LOCUS       DAZGTJ010000001       143457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000001 DAZGTJ010000000
VERSION     DAZGTJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 143457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 143457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..143457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.001"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..146
                     /gene="tdcR"
                     /locus_tag="ACHK8E_000001"
     CDS             <1..146
                     /gene="tdcR"
                     /locus_tag="ACHK8E_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFP0342337.1"
                     /translation="RYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
     gene            402..503
                     /locus_tag="ACHK8E_000002"
                     /pseudo
     CDS             402..503
                     /locus_tag="ACHK8E_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(712..1088)
                     /gene="rnpB"
                     /locus_tag="ACHK8E_000003"
     ncRNA           complement(712..1088)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK8E_000003"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1121..2266)
                     /gene="garK"
                     /locus_tag="ACHK8E_000004"
     CDS             complement(1121..2266)
                     /gene="garK"
                     /locus_tag="ACHK8E_000004"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP0342338.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2363..3253)
                     /gene="garR"
                     /locus_tag="ACHK8E_000005"
     CDS             complement(2363..3253)
                     /gene="garR"
                     /locus_tag="ACHK8E_000005"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0342339.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3283..4053)
                     /gene="garL"
                     /locus_tag="ACHK8E_000006"
     CDS             complement(3283..4053)
                     /gene="garL"
                     /locus_tag="ACHK8E_000006"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP0342340.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4069..5403)
                     /gene="garP"
                     /locus_tag="ACHK8E_000007"
     CDS             complement(4069..5403)
                     /gene="garP"
                     /locus_tag="ACHK8E_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP0342341.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5778..7349
                     /gene="garD"
                     /locus_tag="ACHK8E_000008"
     CDS             5778..7349
                     /gene="garD"
                     /locus_tag="ACHK8E_000008"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP0342342.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7498..7833
                     /gene="prlF"
                     /locus_tag="ACHK8E_000009"
     CDS             7498..7833
                     /gene="prlF"
                     /locus_tag="ACHK8E_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP0342343.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7833..8297
                     /gene="yhaV"
                     /locus_tag="ACHK8E_000010"
     CDS             7833..8297
                     /gene="yhaV"
                     /locus_tag="ACHK8E_000010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP0342344.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8352..9161)
                     /gene="agaR"
                     /locus_tag="ACHK8E_000011"
     CDS             complement(8352..9161)
                     /gene="agaR"
                     /locus_tag="ACHK8E_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP0342345.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9410..10690
                     /gene="kbaZ"
                     /locus_tag="ACHK8E_000012"
     CDS             9410..10690
                     /gene="kbaZ"
                     /locus_tag="ACHK8E_000012"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP0342346.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10713..11186
                     /gene="agaV"
                     /locus_tag="ACHK8E_000013"
     CDS             10713..11186
                     /gene="agaV"
                     /locus_tag="ACHK8E_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP0342347.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11197..11976
                     /gene="agaW"
                     /locus_tag="ACHK8E_000014"
     CDS             11197..11976
                     /gene="agaW"
                     /locus_tag="ACHK8E_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP0342348.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11966..12844
                     /gene="agaE"
                     /locus_tag="ACHK8E_000015"
     CDS             11966..12844
                     /gene="agaE"
                     /locus_tag="ACHK8E_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP0342349.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12862..13296
                     /gene="agaF"
                     /locus_tag="ACHK8E_000016"
     CDS             12862..13296
                     /gene="agaF"
                     /locus_tag="ACHK8E_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP0342350.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13293..14426
                     /gene="nagA"
                     /locus_tag="ACHK8E_000017"
     CDS             13293..14426
                     /gene="nagA"
                     /locus_tag="ACHK8E_000017"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0342351.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            14776..15930
                     /gene="agaS"
                     /locus_tag="ACHK8E_000018"
     CDS             14776..15930
                     /gene="agaS"
                     /locus_tag="ACHK8E_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP0342352.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15943..16803
                     /gene="kbaY"
                     /locus_tag="ACHK8E_000019"
     CDS             15943..16803
                     /gene="kbaY"
                     /locus_tag="ACHK8E_000019"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP0342353.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16970..17446
                     /gene="agaB"
                     /locus_tag="ACHK8E_000020"
     CDS             16970..17446
                     /gene="agaB"
                     /locus_tag="ACHK8E_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP0342354.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17485..18288
                     /gene="agaC"
                     /locus_tag="ACHK8E_000021"
     CDS             17485..18288
                     /gene="agaC"
                     /locus_tag="ACHK8E_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP0342355.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18278..19069
                     /gene="agaD"
                     /locus_tag="ACHK8E_000022"
     CDS             18278..19069
                     /gene="agaD"
                     /locus_tag="ACHK8E_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP0342356.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            19070..19837
                     /gene="agaI"
                     /locus_tag="ACHK8E_000023"
     CDS             19070..19837
                     /gene="agaI"
                     /locus_tag="ACHK8E_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP0342357.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(19887..20750)
                     /gene="rsmI"
                     /locus_tag="ACHK8E_000024"
     CDS             complement(19887..20750)
                     /gene="rsmI"
                     /locus_tag="ACHK8E_000024"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP0342358.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            20815..22851
                     /gene="lpoA"
                     /locus_tag="ACHK8E_000025"
     CDS             20815..22851
                     /gene="lpoA"
                     /locus_tag="ACHK8E_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP0342359.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            22809..23204
                     /gene="yraN"
                     /locus_tag="ACHK8E_000026"
     CDS             22809..23204
                     /gene="yraN"
                     /locus_tag="ACHK8E_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP0342360.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            23224..23814
                     /gene="diaA"
                     /locus_tag="ACHK8E_000027"
     CDS             23224..23814
                     /gene="diaA"
                     /locus_tag="ACHK8E_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP0342361.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            23824..24399
                     /gene="dolP"
                     /locus_tag="ACHK8E_000028"
     CDS             23824..24399
                     /gene="dolP"
                     /locus_tag="ACHK8E_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0342362.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(24512..25552)
                     /gene="yraQ"
                     /locus_tag="ACHK8E_000029"
     CDS             complement(24512..25552)
                     /gene="yraQ"
                     /locus_tag="ACHK8E_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0342363.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(25625..26260)
                     /gene="yraR"
                     /locus_tag="ACHK8E_000030"
     CDS             complement(25625..26260)
                     /gene="yraR"
                     /locus_tag="ACHK8E_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0342364.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            26388..26906
                     /gene="yhbO"
                     /locus_tag="ACHK8E_000031"
     CDS             26388..26906
                     /gene="yhbO"
                     /locus_tag="ACHK8E_000031"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP0342365.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(26886..27329)
                     /gene="yhbP"
                     /locus_tag="ACHK8E_000032"
     CDS             complement(26886..27329)
                     /gene="yhbP"
                     /locus_tag="ACHK8E_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP0342366.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            27380..27682
                     /gene="yhbQ"
                     /locus_tag="ACHK8E_000033"
     CDS             27380..27682
                     /gene="yhbQ"
                     /locus_tag="ACHK8E_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP0342367.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(27669..28172)
                     /gene="yhbS"
                     /locus_tag="ACHK8E_000034"
     CDS             complement(27669..28172)
                     /gene="yhbS"
                     /locus_tag="ACHK8E_000034"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0342368.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(28166..28690)
                     /gene="ubiT"
                     /locus_tag="ACHK8E_000035"
     CDS             complement(28166..28690)
                     /gene="ubiT"
                     /locus_tag="ACHK8E_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP0342369.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            28899..29894
                     /gene="ubiU"
                     /locus_tag="ACHK8E_000036"
     CDS             28899..29894
                     /gene="ubiU"
                     /locus_tag="ACHK8E_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP0342370.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            29903..30781
                     /gene="ubiV"
                     /locus_tag="ACHK8E_000037"
     CDS             29903..30781
                     /gene="ubiV"
                     /locus_tag="ACHK8E_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP0342371.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            30862..31869
                     /gene="yhbW"
                     /locus_tag="ACHK8E_000038"
     CDS             30862..31869
                     /gene="yhbW"
                     /locus_tag="ACHK8E_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFP0342372.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(31987..33231)
                     /gene="mtr"
                     /locus_tag="ACHK8E_000039"
     CDS             complement(31987..33231)
                     /gene="mtr"
                     /locus_tag="ACHK8E_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP0342373.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(33385..35274)
                     /gene="deaD"
                     /locus_tag="ACHK8E_000040"
     CDS             complement(33385..35274)
                     /gene="deaD"
                     /locus_tag="ACHK8E_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP0342374.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(35267..35347)
                     /gene="yrbN"
                     /locus_tag="ACHK8E_000041"
     CDS             complement(35267..35347)
                     /gene="yrbN"
                     /locus_tag="ACHK8E_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP0342375.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(35454..36338)
                     /gene="nlpI"
                     /locus_tag="ACHK8E_000042"
     CDS             complement(35454..36338)
                     /gene="nlpI"
                     /locus_tag="ACHK8E_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP0342376.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(36447..38582)
                     /gene="pnp"
                     /locus_tag="ACHK8E_000043"
     CDS             complement(36447..38582)
                     /gene="pnp"
                     /locus_tag="ACHK8E_000043"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP0342377.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(38829..39098)
                     /gene="rpsO"
                     /locus_tag="ACHK8E_000044"
     CDS             complement(38829..39098)
                     /gene="rpsO"
                     /locus_tag="ACHK8E_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP0342378.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(39247..40191)
                     /gene="truB"
                     /locus_tag="ACHK8E_000045"
     CDS             complement(39247..40191)
                     /gene="truB"
                     /locus_tag="ACHK8E_000045"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP0342379.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(40191..40592)
                     /gene="rbfA"
                     /locus_tag="ACHK8E_000046"
     CDS             complement(40191..40592)
                     /gene="rbfA"
                     /locus_tag="ACHK8E_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP0342380.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(40756..43428)
                     /gene="infB"
                     /locus_tag="ACHK8E_000047"
     CDS             complement(40756..43428)
                     /gene="infB"
                     /locus_tag="ACHK8E_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP0342381.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(43453..44940)
                     /gene="nusA"
                     /locus_tag="ACHK8E_000048"
     CDS             complement(43453..44940)
                     /gene="nusA"
                     /locus_tag="ACHK8E_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP0342382.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(44968..45420)
                     /gene="rimP"
                     /locus_tag="ACHK8E_000049"
     CDS             complement(44968..45420)
                     /gene="rimP"
                     /locus_tag="ACHK8E_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP0342383.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(45627..45703)
                     /locus_tag="ACHK8E_000050"
     tRNA            complement(45627..45703)
                     /locus_tag="ACHK8E_000050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45667..45669),aa:Met,seq:cat)
     gene            46051..47394
                     /gene="argG"
                     /locus_tag="ACHK8E_000051"
     CDS             46051..47394
                     /gene="argG"
                     /locus_tag="ACHK8E_000051"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP0342384.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(47402..49027)
                     /gene="yhbX"
                     /locus_tag="ACHK8E_000052"
     CDS             complement(47402..49027)
                     /gene="yhbX"
                     /locus_tag="ACHK8E_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0342385.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(49484..49570)
                     /locus_tag="ACHK8E_000053"
     tRNA            complement(49484..49570)
                     /locus_tag="ACHK8E_000053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49534..49536),aa:Leu,seq:gag)
     gene            complement(49585..49917)
                     /gene="secG"
                     /locus_tag="ACHK8E_000054"
     CDS             complement(49585..49917)
                     /gene="secG"
                     /locus_tag="ACHK8E_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP0342386.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(50145..51482)
                     /gene="glmM"
                     /locus_tag="ACHK8E_000055"
     CDS             complement(50145..51482)
                     /gene="glmM"
                     /locus_tag="ACHK8E_000055"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP0342387.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(51475..52323)
                     /gene="folP"
                     /locus_tag="ACHK8E_000056"
     CDS             complement(51475..52323)
                     /gene="folP"
                     /locus_tag="ACHK8E_000056"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP0342388.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(52413..54347)
                     /gene="ftsH"
                     /locus_tag="ACHK8E_000057"
     CDS             complement(52413..54347)
                     /gene="ftsH"
                     /locus_tag="ACHK8E_000057"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP0342389.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(54447..55076)
                     /gene="rlmE"
                     /locus_tag="ACHK8E_000058"
     CDS             complement(54447..55076)
                     /gene="rlmE"
                     /locus_tag="ACHK8E_000058"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP0342390.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            55202..55495
                     /gene="yhbY"
                     /locus_tag="ACHK8E_000059"
     CDS             55202..55495
                     /gene="yhbY"
                     /locus_tag="ACHK8E_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP0342391.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(55651..56127)
                     /gene="greA"
                     /locus_tag="ACHK8E_000060"
     CDS             complement(55651..56127)
                     /gene="greA"
                     /locus_tag="ACHK8E_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP0342392.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            56375..57808
                     /gene="dacB"
                     /locus_tag="ACHK8E_000061"
     CDS             56375..57808
                     /gene="dacB"
                     /locus_tag="ACHK8E_000061"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0342393.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(57849..59021)
                     /gene="cgtA"
                     /locus_tag="ACHK8E_000062"
     CDS             complement(57849..59021)
                     /gene="cgtA"
                     /locus_tag="ACHK8E_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP0342394.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(59037..60002)
                     /gene="yhbE"
                     /locus_tag="ACHK8E_000063"
     CDS             complement(59037..60002)
                     /gene="yhbE"
                     /locus_tag="ACHK8E_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0342395.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(60129..60386)
                     /gene="rpmA"
                     /locus_tag="ACHK8E_000064"
     CDS             complement(60129..60386)
                     /gene="rpmA"
                     /locus_tag="ACHK8E_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP0342396.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(60407..60718)
                     /gene="rplU"
                     /locus_tag="ACHK8E_000065"
     CDS             complement(60407..60718)
                     /gene="rplU"
                     /locus_tag="ACHK8E_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP0342397.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            60977..61948
                     /gene="ispB"
                     /locus_tag="ACHK8E_000066"
     CDS             60977..61948
                     /gene="ispB"
                     /locus_tag="ACHK8E_000066"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP0342398.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            62176..62454
                     /gene="sfsB"
                     /locus_tag="ACHK8E_000067"
     CDS             62176..62454
                     /gene="sfsB"
                     /locus_tag="ACHK8E_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP0342399.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(62502..63761)
                     /gene="murA"
                     /locus_tag="ACHK8E_000068"
     CDS             complement(62502..63761)
                     /gene="murA"
                     /locus_tag="ACHK8E_000068"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP0342400.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(63816..64070)
                     /gene="ibaG"
                     /locus_tag="ACHK8E_000069"
     CDS             complement(63816..64070)
                     /gene="ibaG"
                     /locus_tag="ACHK8E_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP0342401.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(64230..64523)
                     /gene="mlaB"
                     /locus_tag="ACHK8E_000070"
     CDS             complement(64230..64523)
                     /gene="mlaB"
                     /locus_tag="ACHK8E_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP0342402.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(64523..65158)
                     /gene="mlaC"
                     /locus_tag="ACHK8E_000071"
     CDS             complement(64523..65158)
                     /gene="mlaC"
                     /locus_tag="ACHK8E_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP0342403.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(65177..65728)
                     /gene="mlaD"
                     /locus_tag="ACHK8E_000072"
     CDS             complement(65177..65728)
                     /gene="mlaD"
                     /locus_tag="ACHK8E_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP0342404.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(65733..66515)
                     /gene="mlaE"
                     /locus_tag="ACHK8E_000073"
     CDS             complement(65733..66515)
                     /gene="mlaE"
                     /locus_tag="ACHK8E_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP0342405.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(66523..67332)
                     /gene="mlaF"
                     /locus_tag="ACHK8E_000074"
     CDS             complement(66523..67332)
                     /gene="mlaF"
                     /locus_tag="ACHK8E_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP0342406.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            67542..68519
                     /gene="yrbG"
                     /locus_tag="ACHK8E_000075"
     CDS             67542..68519
                     /gene="yrbG"
                     /locus_tag="ACHK8E_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP0342407.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            68533..69519
                     /gene="kdsD"
                     /locus_tag="ACHK8E_000076"
     CDS             68533..69519
                     /gene="kdsD"
                     /locus_tag="ACHK8E_000076"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP0342408.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            69540..70106
                     /gene="kdsC"
                     /locus_tag="ACHK8E_000077"
     CDS             69540..70106
                     /gene="kdsC"
                     /locus_tag="ACHK8E_000077"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP0342409.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            70103..70678
                     /gene="lptC"
                     /locus_tag="ACHK8E_000078"
     CDS             70103..70678
                     /gene="lptC"
                     /locus_tag="ACHK8E_000078"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP0342410.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            70647..71204
                     /gene="lptA"
                     /locus_tag="ACHK8E_000079"
     CDS             70647..71204
                     /gene="lptA"
                     /locus_tag="ACHK8E_000079"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP0342411.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            71211..71936
                     /gene="lptB"
                     /locus_tag="ACHK8E_000080"
     CDS             71211..71936
                     /gene="lptB"
                     /locus_tag="ACHK8E_000080"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP0342412.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            71984..73417
                     /gene="rpoN"
                     /locus_tag="ACHK8E_000081"
     CDS             71984..73417
                     /gene="rpoN"
                     /locus_tag="ACHK8E_000081"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP0342413.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            73440..73727
                     /gene="hpf"
                     /locus_tag="ACHK8E_000082"
     CDS             73440..73727
                     /gene="hpf"
                     /locus_tag="ACHK8E_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP0342414.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            73845..74336
                     /gene="ptsN"
                     /locus_tag="ACHK8E_000083"
     CDS             73845..74336
                     /gene="ptsN"
                     /locus_tag="ACHK8E_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP0342415.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            74382..75236
                     /gene="rapZ"
                     /locus_tag="ACHK8E_000084"
     CDS             74382..75236
                     /gene="rapZ"
                     /locus_tag="ACHK8E_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP0342416.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            75233..75505
                     /gene="npr"
                     /locus_tag="ACHK8E_000085"
     CDS             75233..75505
                     /gene="npr"
                     /locus_tag="ACHK8E_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP0342417.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            75718..76350
                     /gene="yrbL"
                     /locus_tag="ACHK8E_000086"
     CDS             75718..76350
                     /gene="yrbL"
                     /locus_tag="ACHK8E_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP0342418.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(76347..77075)
                     /gene="mtgA"
                     /locus_tag="ACHK8E_000087"
     CDS             complement(76347..77075)
                     /gene="mtgA"
                     /locus_tag="ACHK8E_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP0342419.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(77072..77725)
                     /gene="elbB"
                     /locus_tag="ACHK8E_000088"
     CDS             complement(77072..77725)
                     /gene="elbB"
                     /locus_tag="ACHK8E_000088"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP0342420.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(77955..80291)
                     /gene="arcB"
                     /locus_tag="ACHK8E_000089"
     CDS             complement(77955..80291)
                     /gene="arcB"
                     /locus_tag="ACHK8E_000089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP0342421.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(80387..81316)
                     /gene="yhcC"
                     /locus_tag="ACHK8E_000090"
     CDS             complement(80387..81316)
                     /gene="yhcC"
                     /locus_tag="ACHK8E_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP0342422.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            81991..86451
                     /gene="gltB"
                     /locus_tag="ACHK8E_000091"
     CDS             81991..86451
                     /gene="gltB"
                     /locus_tag="ACHK8E_000091"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP0342423.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            86464..87882
                     /gene="gltD"
                     /locus_tag="ACHK8E_000092"
     CDS             86464..87882
                     /gene="gltD"
                     /locus_tag="ACHK8E_000092"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP0342424.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            88065..89192
                     /gene="yhcG"
                     /locus_tag="ACHK8E_000093"
     CDS             88065..89192
                     /gene="yhcG"
                     /locus_tag="ACHK8E_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP0342425.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(89252..89716)
                     /gene="nanQ"
                     /locus_tag="ACHK8E_000094"
     CDS             complement(89252..89716)
                     /gene="nanQ"
                     /locus_tag="ACHK8E_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP0342426.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(89713..90588)
                     /gene="nanK"
                     /locus_tag="ACHK8E_000095"
     CDS             complement(89713..90588)
                     /gene="nanK"
                     /locus_tag="ACHK8E_000095"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0342427.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(90585..91274)
                     /gene="nanE"
                     /locus_tag="ACHK8E_000096"
     CDS             complement(90585..91274)
                     /gene="nanE"
                     /locus_tag="ACHK8E_000096"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP0342428.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(91322..92812)
                     /gene="nanT"
                     /locus_tag="ACHK8E_000097"
     CDS             complement(91322..92812)
                     /gene="nanT"
                     /locus_tag="ACHK8E_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP0342429.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(92922..93815)
                     /gene="nanA"
                     /locus_tag="ACHK8E_000098"
     CDS             complement(92922..93815)
                     /gene="nanA"
                     /locus_tag="ACHK8E_000098"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0342430.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(93937..94728)
                     /gene="nanR"
                     /locus_tag="ACHK8E_000099"
     CDS             complement(93937..94728)
                     /gene="nanR"
                     /locus_tag="ACHK8E_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0342431.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            95108..96475
                     /gene="dcuC"
                     /locus_tag="ACHK8E_000100"
     CDS             95108..96475
                     /gene="dcuC"
                     /locus_tag="ACHK8E_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0342432.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(96519..97016)
                     /gene="sspB"
                     /locus_tag="ACHK8E_000101"
     CDS             complement(96519..97016)
                     /gene="sspB"
                     /locus_tag="ACHK8E_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP0342433.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(97022..97660)
                     /gene="sspA"
                     /locus_tag="ACHK8E_000102"
     CDS             complement(97022..97660)
                     /gene="sspA"
                     /locus_tag="ACHK8E_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP0342434.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(98055..98447)
                     /gene="rpsI"
                     /locus_tag="ACHK8E_000103"
     CDS             complement(98055..98447)
                     /gene="rpsI"
                     /locus_tag="ACHK8E_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP0342435.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(98471..98899)
                     /gene="rplM"
                     /locus_tag="ACHK8E_000104"
     CDS             complement(98471..98899)
                     /gene="rplM"
                     /locus_tag="ACHK8E_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP0342436.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(99118..100245)
                     /gene="zapE"
                     /locus_tag="ACHK8E_000105"
     CDS             complement(99118..100245)
                     /gene="zapE"
                     /locus_tag="ACHK8E_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP0342437.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            100439..100837
                     /gene="zapG"
                     /locus_tag="ACHK8E_000106"
     CDS             100439..100837
                     /gene="zapG"
                     /locus_tag="ACHK8E_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP0342438.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            100991..102358
                     /gene="degQ"
                     /locus_tag="ACHK8E_000107"
     CDS             100991..102358
                     /gene="degQ"
                     /locus_tag="ACHK8E_000107"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP0342439.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            102448..103515
                     /gene="degS"
                     /locus_tag="ACHK8E_000108"
     CDS             102448..103515
                     /gene="degS"
                     /locus_tag="ACHK8E_000108"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP0342440.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(103578..104516)
                     /gene="mdh"
                     /locus_tag="ACHK8E_000109"
     CDS             complement(103578..104516)
                     /gene="mdh"
                     /locus_tag="ACHK8E_000109"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0342441.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            104951..105421
                     /gene="argR"
                     /locus_tag="ACHK8E_000110"
     CDS             104951..105421
                     /gene="argR"
                     /locus_tag="ACHK8E_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP0342442.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            105787..106050
                     /gene="yhcN"
                     /locus_tag="ACHK8E_000111"
     CDS             105787..106050
                     /gene="yhcN"
                     /locus_tag="ACHK8E_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP0342443.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(106105..106377)
                     /gene="yhcO"
                     /locus_tag="ACHK8E_000112"
     CDS             complement(106105..106377)
                     /gene="yhcO"
                     /locus_tag="ACHK8E_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP0342444.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(106469..108436)
                     /gene="aaeB"
                     /locus_tag="ACHK8E_000113"
     CDS             complement(106469..108436)
                     /gene="aaeB"
                     /locus_tag="ACHK8E_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP0342445.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(108442..109374)
                     /gene="aaeA"
                     /locus_tag="ACHK8E_000114"
     CDS             complement(108442..109374)
                     /gene="aaeA"
                     /locus_tag="ACHK8E_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP0342446.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(109382..109585)
                     /gene="aaeX"
                     /locus_tag="ACHK8E_000115"
     CDS             complement(109382..109585)
                     /gene="aaeX"
                     /locus_tag="ACHK8E_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP0342447.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            109768..110697
                     /gene="aaeR"
                     /locus_tag="ACHK8E_000116"
     CDS             109768..110697
                     /gene="aaeR"
                     /locus_tag="ACHK8E_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP0342448.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(110825..112270)
                     /gene="tldD"
                     /locus_tag="ACHK8E_000117"
     CDS             complement(110825..112270)
                     /gene="tldD"
                     /locus_tag="ACHK8E_000117"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP0342449.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(112426..116226)
                     /gene="yhdP"
                     /locus_tag="ACHK8E_000118"
     CDS             complement(112426..116226)
                     /gene="yhdP"
                     /locus_tag="ACHK8E_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP0342450.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(116294..117763)
                     /gene="rng"
                     /locus_tag="ACHK8E_000119"
     CDS             complement(116294..117763)
                     /gene="rng"
                     /locus_tag="ACHK8E_000119"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP0342451.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(117753..118346)
                     /gene="yhdE"
                     /locus_tag="ACHK8E_000120"
     CDS             complement(117753..118346)
                     /gene="yhdE"
                     /locus_tag="ACHK8E_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP0342452.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(118355..118843)
                     /gene="mreD"
                     /locus_tag="ACHK8E_000121"
     CDS             complement(118355..118843)
                     /gene="mreD"
                     /locus_tag="ACHK8E_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP0342453.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(118843..119946)
                     /gene="mreC"
                     /locus_tag="ACHK8E_000122"
     CDS             complement(118843..119946)
                     /gene="mreC"
                     /locus_tag="ACHK8E_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP0342454.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(120012..121055)
                     /gene="mreB"
                     /locus_tag="ACHK8E_000123"
     CDS             complement(120012..121055)
                     /gene="mreB"
                     /locus_tag="ACHK8E_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP0342455.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(121360..123300)
                     /gene="csrD"
                     /locus_tag="ACHK8E_000124"
     CDS             complement(121360..123300)
                     /gene="csrD"
                     /locus_tag="ACHK8E_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP0342456.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            123452..124426
                     /gene="yhdH"
                     /locus_tag="ACHK8E_000125"
     CDS             123452..124426
                     /gene="yhdH"
                     /locus_tag="ACHK8E_000125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP0342457.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            124612..124999
                     /locus_tag="ACHK8E_000126"
                     /pseudo
     CDS             124612..124999
                     /locus_tag="ACHK8E_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            125404..125874
                     /gene="accB"
                     /locus_tag="ACHK8E_000127"
     CDS             125404..125874
                     /gene="accB"
                     /locus_tag="ACHK8E_000127"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP0342458.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            125885..127234
                     /gene="accC"
                     /locus_tag="ACHK8E_000128"
     CDS             125885..127234
                     /gene="accC"
                     /locus_tag="ACHK8E_000128"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP0342459.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            127343..127585
                     /gene="yhdT"
                     /locus_tag="ACHK8E_000129"
     CDS             127343..127585
                     /gene="yhdT"
                     /locus_tag="ACHK8E_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP0342460.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            127575..129026
                     /gene="panF"
                     /locus_tag="ACHK8E_000130"
     CDS             127575..129026
                     /gene="panF"
                     /locus_tag="ACHK8E_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP0342461.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            129038..129919
                     /gene="prmA"
                     /locus_tag="ACHK8E_000131"
     CDS             129038..129919
                     /gene="prmA"
                     /locus_tag="ACHK8E_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP0342462.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            130248..131213
                     /gene="dusB"
                     /locus_tag="ACHK8E_000132"
     CDS             130248..131213
                     /gene="dusB"
                     /locus_tag="ACHK8E_000132"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP0342463.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            131239..131535
                     /gene="fis"
                     /locus_tag="ACHK8E_000133"
     CDS             131239..131535
                     /gene="fis"
                     /locus_tag="ACHK8E_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP0342464.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            131621..132505
                     /gene="yhdJ"
                     /locus_tag="ACHK8E_000134"
     CDS             131621..132505
                     /gene="yhdJ"
                     /locus_tag="ACHK8E_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0342465.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            132589..132768
                     /gene="yhdU"
                     /locus_tag="ACHK8E_000135"
     CDS             132589..132768
                     /gene="yhdU"
                     /locus_tag="ACHK8E_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP0342466.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(132771..133433)
                     /gene="acrS"
                     /locus_tag="ACHK8E_000136"
     CDS             complement(132771..133433)
                     /gene="acrS"
                     /locus_tag="ACHK8E_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP0342467.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
     gene            133832..134989
                     /gene="acrE"
                     /locus_tag="ACHK8E_000137"
     CDS             133832..134989
                     /gene="acrE"
                     /locus_tag="ACHK8E_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP0342468.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            135001..138105
                     /gene="acrF"
                     /locus_tag="ACHK8E_000138"
     CDS             135001..138105
                     /gene="acrF"
                     /locus_tag="ACHK8E_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP0342469.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            138358..138579
                     /locus_tag="ACHK8E_000139"
     CDS             138358..138579
                     /locus_tag="ACHK8E_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342470.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            139010..140035
                     /locus_tag="ACHK8E_000140"
     CDS             139010..140035
                     /locus_tag="ACHK8E_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0342471.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            140103..141284
                     /gene="yhdX"
                     /locus_tag="ACHK8E_000141"
                     /pseudo
     CDS             140103..141284
                     /gene="yhdX"
                     /locus_tag="ACHK8E_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            141294..142397
                     /gene="yhdY"
                     /locus_tag="ACHK8E_000142"
     CDS             141294..142397
                     /gene="yhdY"
                     /locus_tag="ACHK8E_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0342472.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            142405..143163
                     /gene="yhdZ"
                     /locus_tag="ACHK8E_000143"
     CDS             142405..143163
                     /gene="yhdZ"
                     /locus_tag="ACHK8E_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP0342473.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(143226..143341)
                     /gene="rrf"
                     /locus_tag="ACHK8E_000144"
     rRNA            complement(143226..143341)
                     /gene="rrf"
                     /locus_tag="ACHK8E_000144"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(143381..143456)
                     /locus_tag="ACHK8E_000145"
     tRNA            complement(143381..143456)
                     /locus_tag="ACHK8E_000145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(143421..143423),aa:Thr,seq:ggt)
BASE COUNT        34986 a        38059 c        35949 g        34463 t
ORIGIN      
        1 cacgctattt gttggagaac aaacatttat tttatcaata ttttaaaatt tcgaatacag
       61 gtatcgatca tctcgaacaa ttgattaacg tcaacttttt ctcttctgac aggacgtcat
      121 tttgtgaatg caatcgtttt ccataaattc ctctcccctc ataggcgacg aatagcattt
      181 tgtgttgagg atcacaaaac gaataattgc tggtcgccgc gataaggtca gacgaagaca
      241 acaaggggga ttctcactgt gctttacatc agcgtaggcc atagaatgtt gcttcgttga
      301 ctgtaatgcc gggtaaatga gcattttttg atagtgcgaa agaaccctgc gaggaaaata
      361 agcatctatt attgtttgtc atgatgacaa ggcaggcatc aatgaaaggc ttcccaattg
      421 cacatatttt tcacccttca atcccgccaa tgcacgcagt ggttaacaat cacaacagaa
      481 atattgatta ttggacggta aaaatttagg ttataacgtc caaatgattc aacgatcgga
      541 caatacctgc gaacttacaa ttaatgagag aaatatatgt ataattacga ccttttaaat
      601 gcaatataga cttaataact tcttacaaag tctattatta ataccgtaga tattcatctt
      661 accctgggca ccaaaagcaa aaacccgccg aagcgggttt ttacgtaaat caggtgaaac
      721 tgaccgataa gccgggttct gtcgtggaca gtcattcatc taggccagca atcgctcact
      781 ggctcaagca gcctacccgg gttcagtacg ggccgtacct tatgaacccc tatttggcct
      841 tgctccgggt ggagtttacc gtgccacgga ctgttaccag ccgcgcggtg cgctcttacc
      901 gcaccctttc acccttacct gatcccgctt acgcgggcca tcggcggttt gctctctgtt
      961 gcactggtcg tgggtttccc ccccaggcgt tacctggcac cctgccctat ggagcccgga
     1021 ctttcctccc ctccgcccgt ctcccccgaa gaggacgacg acgaagcggc gactgtctgg
     1081 tcagcttcgg cgcgcagtat agagggtttg cgcgcccttg tcaccccgcg ttgcgcattc
     1141 caatcgccag tgtcgcggcg atattacgtg aagcacggca gatattgtca taagccccgc
     1201 ggaatgcttc gtccaacgta ccaatgctgg tcaatacgct gaagaccgca tcaatgccat
     1261 gctgatgtac aacgccaaca tcattggtca ggctacccgc aatgccaatc accggtttat
     1321 ggtacttctt cgccacgttt gcgacaccaa tcggtacctt gccgtgaata ctctggctat
     1381 caatacgccc ttcaccagtg atcaccagcg tacaatcgtg aatatgttcc tccagattca
     1441 gcgccgtagt gacgatttca ataccacttt tcagttctgc accaagaaac gccatcagcg
     1501 ccgcgcccat accgcccgca gctcctgcgc cggggacatc tttcacatca acatgcagcg
     1561 cttttttaat aacatcggca tagtgagaga ggttattgtc cagctcgaca atcatcgcct
     1621 cactggctcc cttttgtggg ccaaagatgc gcgaagcacc actgtcgccg actaacgggt
     1681 tggtgacatc acaagcgacg cggatgacac aatcttttaa gcgcggatcg agaccggaaa
     1741 tatcaatatc attcagagta ttaagactac cgccgccaaa accaatttca ttgccgttgg
     1801 catcgcataa tttcgccccc agcgcctgta ccatgcctgc gccgccgtcg ttggtggcgc
     1861 tgccgccaat gccgataata atgtttgtcg caccgctctc cagcgcctgc aggattaact
     1921 caccagtacc gcgtgaagtg gtcacgagtg gatcgcgttt ttccgcaggt accagttcca
     1981 gcccactggc cgccgccatt tcgataaacg cggttttgcc atcgccggag atcccccaac
     2041 tggcattcac tttctcgccc agcggccctg taacccaggc gtgacgttca gccccctggg
     2101 tggctgcaat catcgcttcc accgttcctt caccaccgtc ggcaaccgga acagaaacat
     2161 actgtgcatc aggaaaaatt tcccgaaatc ctttttctat cgcctgcgca acctcgctgg
     2221 cagataaact ttctttataa gagtctgggg cgattacgat tttcatacct atgcctgtta
     2281 ccacatgacg ccggagggcg tttctcttat tcggcctgga ttccaggccc ggattgcaat
     2341 acgccatccg ggcacgacgt cattagcgag taacttcgac tttcgccagt ttttcgtagt
     2401 agcacgccag ggcgctatga tccgccgttc ctaaaccatc tgcacgcagt gcctgcatca
     2461 tctccataac cgcagctgtg agcggcagtt gcgcgccgac gccgtgagaa gtatccagcg
     2521 cattcgccag atccttaata tgcagatcaa tacggaagcc cggtttgaag ttgcggtcca
     2581 tcaccatcgg cgctttggca tccagcacgg tactgcccgc cagcccaccg cgaattgcct
     2641 gatacaccag atccgggtta acgcccgctt tggttgccaa cgttaacgct tctgacatcg
     2701 cagcaatatt cagcgccaca atgacctgat ttgccagttt ggtgacgtta cctgcaccga
     2761 tttccccggt atgcaccacg gaacccgcca tcgctttcat caaatcatag tatttgtcga
     2821 aaatagcctt gtcgccgccc accatcactg acagcgtacc gtcgatggct ttcggttcac
     2881 cgccgctcac cggagcatcc agcatatcaa tgcctttcgc tttcagcgct tcgctgattt
     2941 cacggcttgc cagcggtgcg atagaactca tatcgatcaa taccgtacct ggcttcgcgc
     3001 cttcaataat gccattctca cccagcgcca cctctttcac atgaggggag tttggcagca
     3061 tggttatgat tacgtcgcac tgttcagcga tcgctttagc cgtagaggct gtttctgcac
     3121 ctgcagcaat cacgtcagcg atagcttctg ggttacggtc agcaaccacc agcgagtaac
     3181 ctgctttcaa aaggttttta ctcattggtt tacccataat cccaaggcca ataaaaccaa
     3241 ctttcatagt catatcaatc atctctctta ttgcggtggt ggttattttt taaaggtatc
     3301 agccagtttc tgagtggcag agcggaagac gccgagatcg ctgccgacgg ccacaaacgt
     3361 cgcgccccat tccagataac gacgcgcatc agcctcgact ggtgcgagga tgccgctggg
     3421 tttgccgtgc gcgctggcac ggttaaaaat gtgctgaatt gctttttgta catccgggtg
     3481 cgaggcattg ccgagatggc ctaatgccgc agccagatcg ctggggccga cgaagatgcc
     3541 gtctacacct tcggtagcgg caatagcatc gacgttatct acgccctgct gactttctat
     3601 ctggaccaga atagtgatgt tcttgttcga ctgagcgaaa taatccgcca cggtgccaaa
     3661 catattggcg cggtgagaaa cggagacgcc gcgaatgcct tccggtgggt aacgagttga
     3721 tgccaccgcc tgctctgctt cctcttttgt ttctacaaaa ggaatcagga agttatagaa
     3781 accgatatcc agaagacgct taataattac cggctcgttg gtcggcactc gcactactgg
     3841 cgcgctggcg ctgcctttca gggccattaa ctgcggaata aacgtggaaa tatcgtttgg
     3901 cgcatgttca ccatccagca ccagccagtc aaacccagcc aaaccaagaa cttcagtgct
     3961 aatcgggtta gagagagctg accagcaacc aatttgtacc tgtttcgcag ccagtgcggc
     4021 tttgaattta ttcgggaaaa catcgttatt catcgcttat acctttgctt atttctgcaa
     4081 ttccatacgt ttaatgtcgc cgactacgaa gaggtagcag accatcgcca tcagcgctga
     4141 acatcccaca aaaaccagtg ctgcattgaa ggagtgcagt tcacttacca ggtagccaat
     4201 caccagtgga gtgacaatgg aggcaacatt gccaaagacg ttaaagacgc cgccgcagag
     4261 gccaacaatc tctttcggcg cggtgtcaga aatcactggc cagcccagcg caccaaatcc
     4321 tttaccaaag aaagccagcg ccatcagcat gaccaccagc gtggtgttgt tggtgtagtt
     4381 acataagatg atggtggaag ccagcaacat tcccagcaca attggtagct tacgtgccag
     4441 ggtcagggac aaaccgcgtt tgatcagata atccgagaag acacctccca gcacgccgcc
     4501 cgcaaagcca cacagtgccg gaatcgaggc gaccagaccc actttcagaa tcgacatgcc
     4561 tttttcctgc accagataaa ttgggaacca ggtgaggaag aaccaggtga tggtgttgat
     4621 aaaatattgt ccgaaaaata cgcccagcat catgcggtta gagagcaatt gcttgatgta
     4681 atgcagtttg ggtccgcttg ctgccgcact gcccggcttt ttgtggtcca tatcgaccac
     4741 cgcgccattt tccgagataa acttcagctc ttccgcagac atacgtgggt gatctgtcgg
     4801 gttatgaatc aacttgatcc acagcgccgt cagcacaaaa ccaattaccc ccataacggt
     4861 aaagacgtgc tcccagcccc acgcgaaagt cagccagcca agcagcggcg aaaagagcgc
     4921 cagcgagaaa tattgcgccg agttaaagat ggcggaggca gtaccacgtt ctttcgtcgg
     4981 gaaccaggcg gcgacaatac gggcgttcgc cgggaatgat ggcgcttccg agaaccccag
     5041 cataaagcgc ataaagaaca tggagatccc tgcccaggcc agcgggaaca tatcaacaaa
     5101 gccttgcagg aaggtgaata gcgaccagaa aaagaggctg taggtgtaaa cttttttcga
     5161 accaaactta tcaagcagcc agccgccggg gatttgcatc agcaagtagg cccagccaaa
     5221 agcagagaag atgtaaccca tcgaaaccgc acttaactgc aactcttttg ccacttcggt
     5281 accagcaata gacagcgttg cacgatcggc gtagttaacg gcggtaacaa taaaaataat
     5341 cagtaatatt aaatagcggg tatgcacgcc tttctttttt tcgtcaacgg tgtccagcat
     5401 cattttattt acctcgggta cttatgctga tttttattat tatggggaag gtgttattta
     5461 tgagtttcat ttatgccgtt acgacaatga actcgggaat tagtataagc agcgcgagaa
     5521 taataatcat tgtgcaaatg ctaatttaat tagtactatt taaatattat tttgagcata
     5581 tgcacataag gttgcgcgct aaagcacaga tttgcgcttt accttgccgg gcggcactgc
     5641 aatccctgaa atgattgaca ttgatcacat ttctgcattt aaactcctga cattcttatt
     5701 tcacccaatg aagttattta tttttaaatg agaccaggtc ctcattttaa taacccctgg
     5761 ctggagaata ttgcacaatg gccaacatcg aaatcagaca agaaacgcca actgcgtttt
     5821 atataaaagt tcacgacaca gataatgtgg caattattgt taatgataat ggcctgaaag
     5881 caggaacgcg ttttccggat gggctggaat taattgaaca tattccccag gggcataaag
     5941 tcgcattgct ggacattccg gctaatggtg aaattattcg ttatggcgaa gtgattggtt
     6001 acgccgtgcg cgcaatccca cgcggaagct ggatcgacga atcaatggtt gtactaccgg
     6061 aagcgccgcc gttacacacg ctgccactgg caaccaaagt cccggcaccc ttaccgccac
     6121 tggaaggata cacctttgag ggctatcgca atgccgatgg cagcgtgggc accaaaaacc
     6181 tgctcggcat caccaccagc gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa
     6241 tcattgaacg cgatctgctg ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc
     6301 atctgtacgg ttgtggcgtg gcgattaacg ctccggcggc ggtggtgccc attcggacca
     6361 ttcacaacat ttcgcttaac ccaaactttg gcggcgaggt gatggtgatt ggcctgggtt
     6421 gcgagaaatt acagccagaa cgcctgctgg tcggcacgga tgatgtacaa ggtattccgc
     6481 tggagaacgc cagtattgtc agcttgcagg atgaaaagca tgtcggtttt cagtccatgg
     6541 tcgaggatat tttgcaggtc gccgaacgcc atttgcacaa actgaatcaa cggcagcgag
     6601 aaacctgtcc agcttcagaa ctggttgtcg gcatgcagtg cggtggcagc gatgcgtttt
     6661 ctggcgtaac ggcaaacccg gcggttgggt atgcgtctga tctactggtg cgctgcggcg
     6721 caacggtgat gttttcagaa gtcacggaag tgcgtgacgc gatccatctg ctgacaccac
     6781 gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga gatggagtgg tacgataact
     6841 atctcaatat gggaaaaacc gaccgtagcg ccaacccttc gccgggcaac aagaaaggcg
     6901 gtctggcaaa tgtggtggag aaagcgctcg gctccattgc taaatcgggt aaaagcgcaa
     6961 ttgttgaagt gctgtcgccc ggtcaacgcc cgactaaacg cggattaatt tacgccgcga
     7021 cgccagccag cgattttgtc tgtggcacac aacaggtggc ttcagggatt accgtgcaag
     7081 tgtttacgac cggtcgcggt acgccgtacg gcctgatggc gatacccgtc attaaaatgg
     7141 cgacccgcac cgagctggcg aaccgctggt ttgatttaat ggatattaac gcgggcacta
     7201 tcgctaccgg cgaagaaacc attgaagaag tgggctggaa gttgttccac tttattctcg
     7261 acgtcgccag cgggaagaag aaaactttct cggatcaatg gggactgcat aaccagctgg
     7321 cggtgtttaa cccggcacca gtgacctgat ttcttttgga acgtccctcg caaaacatgg
     7381 ccttagtgcc atgtttttat tgtttaaagt ccacacatcc attaataatg catttgcatt
     7441 accttagttc aagcttataa tttgagcaga aaacaggctg taaaaggaca gtaaataatg
     7501 cccgctaatg ctcgctctaa cgctgtactg accactgaat caaaggtcac tatacgcgga
     7561 caaacaacta tccccgcgcc agtgcgtgag gccttaaaac tgaagccagg cctggacagc
     7621 attcattacg aaattctgcc tggtgggcaa gtatttatgt gccgactggg agatgaacag
     7681 gaggatcata ctatgaatgc atttttgcgt tttctggatg cagatatcca gaacaacccg
     7741 caaaaaactc gtccattcga cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc
     7801 aacattgacg atgagattgg cgacgacgaa taatggattt tccacaaagg gttaatggtt
     7861 gggcgctata tgctcatccc tgttttcagg aaacctacga tgctttagtt gccgaagtcg
     7921 aggcattaaa gggaaaagat cctgaaaatt atcagagaaa agctgccaca aagttattgg
     7981 cggtggtcca taaagtgatt gaggagcata tcacggtcaa tccatcatca ccggcattcc
     8041 gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg gtcacgggta aaatttggtg
     8101 ctggtcgcta tcgtctcttc tttcgttaca gtgaaaaaga gaaagtcatc attctgggat
     8161 ggatgaacga tgaaaacacg ctgcgcacct acggtaaaaa aacagatgcc tataccgtat
     8221 tcagtaaaat gttaaaaaga ggacatcctc ctgccgactg ggaaagcctc acccaagaga
     8281 cagaagaaaa ccattgatgg tgttcacaat gcccgccgaa cgcattggtg ttgaccagcg
     8341 ggcatccttt ctcattcccc gaccagaatc acttcaaccc cagcctttct cagtccttcc
     8401 agactatccg caggaatgcc ttcatcaaca atgatcatgt cgatacgttg agtatcaatg
     8461 atcttatgta aactagaacg attgaactta ctggaatcgg tgaccacgat gatccgttcc
     8521 gcaacttcgc acatccgacg gtttaaacgg gcttcatctt cattatgcgt gctgacgccg
     8581 cgctccagat cgatcgcatc tacaccaaga aacagcatat cgaagtggta attttgcagc
     8641 gattgctcag cctgatcacc gtaaaaagat tgcgactgac ggcgcaaatg ccccccggtc
     8701 atcagcagct caacgccttc cgcttccagc aacgcattag ccacgttcat accgttggtc
     8761 atcgcaatta cgtcggtgtg cttgcgcatc agacgagcaa tctcaaaagt ggtggtcccg
     8821 gagtcgagga tcacccgatg accaggctga atcaactcaa cggcagcttt cgcaacgctg
     8881 cgtttcatcg cggtgttcag cgcgctttta tcttccactg atggctcgac tgacggcgtt
     8941 gtgctatcgc agatcaacgc gccaccatag gcacgcacag cgatcccctg cttttccaga
     9001 aacgccagat cgttgcggat cgtcacagta gatacaccat acaacgccga tagatcatta
     9061 acttgcacac ttccttgctg gcgcaggcgt tgaatgatct gctctcgtcg ctcgctggtg
     9121 cctgtcactc gcttctcacc tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa
     9181 ctttcgtttc atttcgtttt gcctattaac gcctttctat taagcaaatg caagcccacc
     9241 ttacccattg gcgcaagcta ctctcgtttc agtggctttc attatgtttc ttttgtgaat
     9301 cagatcagaa aaccattatc tttcgtttta tttttatctc accatgacgc agtatcaact
     9361 gaaacaaaac gaaagattaa tgtcgcagta atctgaactg gagaggaaag tgaaacatct
     9421 gacagaaatg gtgagacagc acaaagcggg caaaacaaat gggatttatg ccgtttgttc
     9481 cgcacatccg ctggtgctgg aagctgcaat ccgctacgcc agtgcaaacc aaacgccgtt
     9541 actgattgaa gcaacctcca atcaggtgga ccagttcggc ggttataccg gaatgacgcc
     9601 cgccgatttt cgcggctttg tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc
     9661 gctgattctg ggtggtgacc atctggggcc aaaccgctgg caaaacctgc ctgccgctca
     9721 ggcaatggcc aatgccgatg atttgattaa aagctacgtg gcggcaggat tcaaaaaaat
     9781 tcaccttgat tgcagcatgt cctgtcagga cgatccgatt cccttaaccg atggcatcgt
     9841 ggccgaacgc gccgcccgtc tggcgaaagt ggcggaagaa acctgtcttg aacacttcgg
     9901 cgaagccgat ctggtgtatg tcattggcac cgaagtaccg gtacctggcg gcgcgcatga
     9961 aaccttaagc gagctggcgg tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca
    10021 tcgtcacgcc tttgaaaagc aaggtctgag tgccatctgg ccacgcatca ttgctctggt
    10081 ggtacaaccc ggcgtcgaat tcgatcacac caacgttatt gattatcagc ccgtcaaagc
    10141 gaccgcctta agccagatgg tcgaaaacta cgaaacgctg attttcgaag cgcactctac
    10201 cgattaccaa acgccgcaat cgttgcgcca gctagtgatt gaccactttg ccatcctgaa
    10261 agttggcccg gcgctgacct tcgccctgcg tgaagctctg ttctctctgg cggcgattga
    10321 agaagaactg gtaccagcaa aagcctgttc tggtctgcgt caggtgctgg aaaacgtgat
    10381 gctcgaccgc ccggaatact ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct
    10441 ggcgcgtggt tatagctact cggatcgcgt gcgctattac tggccggaca gccagattga
    10501 tgacgctttc gctcatctgg ttcgtaatct ggcggattca ccaattccgc tgccgctgat
    10561 cagtcagtat ctgccgctgc agtacgtgaa agttcgctcc ggcgagctgc agccaacgcc
    10621 acgggaactc attatcaacc atattcagga catcctggcg cagtaccaca cagcctgtga
    10681 aggccaataa gcaaaacaaa gaggaacacg ctatgccaaa tattgtttta agtcggattg
    10741 atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc
    10801 tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac ctgatggaaa
    10861 tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtc atcgacaaca
    10921 ttcatcgcgc cgccgatcga cagaaaatcc tgctagtttg taaaacaccg gccgatttcc
    10981 tgacgttggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat atgcactacg
    11041 ccaatggcaa gcaacaaatc gccaaaacgg tttctgtgga tgcgggtgat atcgcagcat
    11101 ttaacgatct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg acagagcctg
    11161 ctgtagacct ctttaaatta ctttgaggga ttcatcatgg aaatcagtct gttgcaggca
    11221 tttgcgttgg gaattatcgc ctttatcgct ggcctggata tgtttaacgg cttaacccat
    11281 atgcaccgcc cggtggttct cggcccgttg gtcgggctgg tacttggcga tctgcatacc
    11341 ggtattttaa ccggcggtac gctggaactg gtgtggatgg ggctggcccc tctggcgggc
    11401 gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc cattactact
    11461 ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg
    11521 gggattacct tcctgttctc ggtgatgtcc ggcgtgatgt cccgatgcga ccggatggcg
    11581 gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcattgct ggcactcggc
    11641 acgttctatt ttctctgcgc tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc
    11701 atcattgatg tcctgccgca acgattaatt gatggcctcg gcgtcgcggg cggcatcatg
    11761 ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta catcccctac
    11821 ttcatcctgg gctttgttgc cgcggcctgg ctcaagttac cggtgctggc tattgctgcc
    11881 gctgcgctgg cgatggcgct gatcgacctg ctgcgtaaat ctcctgaacc gactcaacct
    11941 gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc cgaacgtcag
    12001 cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag atcagagcat
    12061 tggcgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca
    12121 ggcttctttc aactacgaac gtatgcaggc ttccggctgg ctgtacggcc tgctgccagc
    12181 actgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg ggcatatggg
    12241 tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc ttgcgatgga
    12301 gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg gtgcgccgct
    12361 cggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg gcgggatagg
    12421 tgcaagcctc gcgctacaag gctctattct tggcgctgtc gtctttattg tgctgttcaa
    12481 cgtggtgcac ctggggctgc gttttggtct ggcgcattat gcttaccgca tgggcgtggc
    12541 ggcgattcca ctcattaaag caaataccaa aaaagtcggc catgcggcat ctatcgttgg
    12601 gatgacggta atcggcgcgc tggtggcaac ctatgttcgt ttaagcacca cgctggaaat
    12661 caccgcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac tgatgccagc
    12721 cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg gctggagtcc
    12781 gctgcgcctg attgcagtga ccgtggttct cggcatcgtc ggtaaattct gccatttcct
    12841 ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg cgggtttgcc
    12901 agtggcatgg aaaaagcaat gaagcaaatc ctcggcgagc aatcgcagtt tattgccatc
    12961 gattttccgg aaacctcaag caccgcgctg cttacatcgc agcttgaaga ggccatcgcc
    13021 caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg cacgccgttt
    13081 cgcgtagctt caacgctggc gatgcaaaaa ctgggctgtg aagtgattac cggcaccaat
    13141 ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga agagtttcgt
    13201 gtgcaggcgc tggagtgtgg acatcgtggg ctgaccagtc tggtggacga gttaggccgc
    13261 tgccatgagg aatgtccggt cgaggaagga atatgacacc cgttttgcgc gccagaaggc
    13321 tgctgactga agagggatgg ctcgatgacc atcagttgcg tattgctgac ggtgtaatcg
    13381 cagaaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg
    13441 cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat gacgcgccgg
    13501 atgtgctcga caagctggca atgcacaaag cacgcgaagg tgtcggcagt tggttaccga
    13561 ccaccgtaac cgcgccgctt aataccattc atgcggcgct ggaacgtatt gctcaacgtt
    13621 gccaacgcgg cggacctggt gcacaaatac tggggagtta tctcgaagga ccgtacttca
    13681 cgccgcagaa taaaggcgcg catccgccgg agctgtttcg cgagctggaa attgccgagc
    13741 tggatcaatt gattgccgtt tctcagcaca ccttgcgcgt ggtggcgctg gcaccggaaa
    13801 aagaaggggc attgcaggcc atccgccatc ttaaacagca aaacgtaagg gtgatgctgg
    13861 ggcatagcgc ggcgacctgg caacaaaccc gcgccgcgtt tgatgctggt gccgacggcc
    13921 tggtgcattg ctataacggg atgacaggtt tacatcaccg cgaaccggga atggttggcg
    13981 cgggattaac ggacaagcgc gcctggctgg aactgatagc cgatggtcat catgtgcatc
    14041 cggcggcgat gtcgctgtgt tgttgctgtg cgaaagagag aatcgtgctc atcaccgacg
    14101 cgatgcaggc agccgggatg ccggatggtc gctatacgtt atgtggcgaa gaagtgcaga
    14161 tgcacggtgg cgttgtccgt accgcgtccg gtggactggc gggcagtacg ctgtctgtcg
    14221 atgcggcagt gcgcaacatg gtcgagttga cgggcgtaac gcctgcggaa gccatccata
    14281 tggcgtcgct gcatccggcg cgtatgctgg gtgttgatga tgttctggga tcgcttaaac
    14341 cgggcaaacg cgccagcgtc gttgcgctgg atagcgggct gcatgcgcaa caaatctgga
    14401 ttcagggtca attagcttcg ttttgatggt ttgctccttt attgggcctt cacttccccc
    14461 gtaaggcctt tctttttctt tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaat
    14521 gcgccttatc cgacctacgg ttggtgaccg taaggccgga taaagtattt acgccgcatc
    14581 cagcaatccc ttttacttcc cttgtctttt ctttcaacga tcacagattt cgttttattt
    14641 cctttttctc cattgaactt tcagtttctt ttctatagat tttaaccaac gaaagaaatc
    14701 accaagtgaa atgaaacgaa aggcaaacga aagcgacaac gccgacgtca agttcatcag
    14761 actaaggatt gagttatgcc agaaaattac acccctgctg ctgccgcaac cggtacatgg
    14821 actgaagaag agatccgcca tcagcctcgc gcatggatcc gttcactcac caacatcgac
    14881 gcgctacgtt ccgcgctcaa taacttcctt gaaccgttac tgcgcaaaga gaatctgcaa
    14941 atcatcctga ccggagccgg aacatcggca tttatcggtg acatcatcgc gccgtggctc
    15001 gccagtcata ccggtaaaaa cttcagcgcc gtaccgacca ccgatctggt cactaatccg
    15061 atggactacc tgaatccagc tcatccgctg ctgttgatct ccttcggtcg atccggcaac
    15121 agcccggaaa gcgtcgccgc cgtggaactg gcaaatcaat ttgtacccga gtgctatcac
    15181 ctgccgatca cctgcaacga agcgggcgct ctttaccaaa acgcgatcaa cagcgacaac
    15241 gcgtttgccc tactgatgcc cgcagaaacg cacgaccgcg gctttgcgat gaccagcagc
    15301 attaccacca tgatggccag ttgcctcgcg gttttcgcac ctgagacgat caacagccag
    15361 agcttccgcg acgtggcgga tcgttgccag gcgatcctga cctcactggg cgatttcagc
    15421 gaaggtgtgt ttggttacgc accgtggaaa cggatcgttt atctcggcag cggtggctta
    15481 cagggcgcag cacgcgagtc ggcgctgaaa gtgctggaac tgacagcggg taaactggcg
    15541 gccttctatg attccccgac cggattccgt catggcccga aatcgctggt cgataacgaa
    15601 acgctggtgg tggtatttgt ctccagccac ccttacaccc gtcagtatga tcttgatctg
    15661 ctggcagaac tccgccgcga taaccaggca atgcgcgtaa tcgccatcgc cgcggaaagc
    15721 aatgacgtta ttactgccgg tccacatatc atcctgccgc cgtcccgtca ctttatcgac
    15781 gttgagcagg cattttgctt cctgatgtac gcccagacat ttgcactgat gcagtcgctg
    15841 cacatgggta atacaccgga taccccatca gccagtggca ccgttaaccg cgtggtgcaa
    15901 ggcgtaatca ttcatccgtg gcaggcataa gaggatcgca ttatgagcat tatctccacc
    15961 aaatatctgt tacaggacgc ccaggccaat ggctacgcgg tgcctgcctt taacattcac
    16021 aacgccgaga cgatccaggc gatcctcgaa gtgtgcagtg aaatgcgatc accggtgatc
    16081 ctcgccggga cgccggggac ctttaagcat atcgcgctgg aagagatcta cgccctgtgt
    16141 agcgcctatt ccacaaccta caacatgccg ctggcactgc atctcgacca ccacgaatcg
    16201 ctggatgata ttcgccgtaa agtccacgca ggcgtgcgca gcgcgatgat cgacggcagt
    16261 cacttcccgt ttgccgagaa cgtgaagctg gtgaaatcgg ttgtcgactt ctgccactcc
    16321 caggattgca gcgtggaagc agaactgggc cgcctcggcg gtgttgaaga tgacatgagc
    16381 gttgacgccg aaagtgcatt cctgaccgat ccacaagaag ctaaacgctt tgtcgaactg
    16441 actggcgtcg acagcctggc ggtagcgatt ggtacggcgc acggcttata cagcaaaacg
    16501 ccgaagattg atttccagcg gctggcggaa attcgtgaag tggtggatgt tcctctggtg
    16561 ctgcatggtg ccagcgatgt cccggatgaa tttgtccgtc gcactattga acttggcgtc
    16621 acaaaagtga acgttgccac agaattaaaa atagccttcg ctggcgcggt taaagcctgg
    16681 tttgcggaaa atccacaggg taatgatcct cgttattata tgcgcgtcgg tatggatgcg
    16741 atgaaagaag ttgtcagaaa taaaattaat gtctgtggtt cagcgaatcg aatttcagca
    16801 taatcattag ttttttatga tttatcccaa tgtacttccc gattaatatc ggggagtgcc
    16861 ttaatggtaa aggagataac taaaccttaa taaatacatc agtaaaatat cgcaacaata
    16921 atatatttaa aaaaattata ttattcaact ttatggtgag gatcacacaa tgagcagtcc
    16981 aaatattctc ttaacccgta ttgataaccg tctggttcac ggtcaggttg gcgtgacctg
    17041 gacatccacc atcggtgcga atctgctggt agtcgtggat gatgttgtcg ctaacgatga
    17101 tattcaacag aaattaatga gtattaccgc agaaacctac ggctttggca ttcgtttctt
    17161 tactatcgaa aaaaccatta acgtcatcgg caaagctgca ccgcatcaaa agatcttcct
    17221 gatttgccgt acgccacaaa cggtacgtaa attggtagaa ggtggtattg acctgaaaga
    17281 tgtcaacgtc ggcaatatgc atttctcgga agggaaaaag caaatcagca gtaaagttta
    17341 tgtcgatgac caggatctca cggacttacg ttttattaaa caacgtggcg tgaatgtttt
    17401 cattcaggac gtccctggcg atcaaaaaga acaaatccct gactaaatct aaaatcgcct
    17461 taatattggt ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg attatccctg
    17521 gcggcgttag ttttcgttct ggggattgat ttttggctgg aagccttatt tttattccgc
    17581 ccgataatcg tttgtaccct aactggcgct attctcggtg atattcagac tggcttaatt
    17641 accggtggtc tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg tgttcagccg
    17701 cccaacccga ttatggcggg tctgatgacc accgtcattg catggtctac gggcgttgat
    17761 gccaaaacag caattggtct tggcctgccg tttagtttgt taatgcagta tgtcattctg
    17821 ttcttctatt ctgctttctc attatttatg accaaagccg ataaatgcgc gaaagaagcg
    17881 gatacggcag cgttttcccg acttaactgg acaacgatgc tcatcgttgc ttcagcgtat
    17941 gcggtgattg ctttcctctg tacttacctg gcacaagggg cgatgcaggc gctggtgaaa
    18001 gcgatgcccg cctggctgac ccacggcttt gaagtggctg gcggtattct gcctgccgtt
    18061 ggttttggct tgctgctgcg cgtgatgttc aaagcgcaat atatccctta cctgatcgcc
    18121 ggcttcctgt ttgtttgcta catccaggtc agcaacctgt tgccggttgc cgtactgggc
    18181 gcaggctttg cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc gcaaccgcag
    18241 cccgttgcca gtaaaaatga agaagaggac tacagcaatg ggatctgaaa tcagtaaaaa
    18301 agatatcacc cgtctgggct ttcgttcgtc actgctacaa gcaagcttta actacgaaag
    18361 gatgcaggcg ggcggcttta cctgggcgat gttgccgctc ctgaaaaaga tttataagga
    18421 cgacaaacca ggcctaagcg cggcgatgaa agataaccta gaatttatta atacccaccc
    18481 gaatctggtc ggattcctga tggggttatt aatttcgatg gaagagaaag gggaaaaccg
    18541 cgacaccatt aaaggcctca aagtggcgct gtttggccca atcgccggga ttggcgatgc
    18601 gattttctgg tttaccttgt taccaattat ggcggggatt tgctcatcgt ttgccagtca
    18661 gggaaacctg ctggggccga ttctgttctt tgccgtttac ctgcttatct ttttcttgcg
    18721 cgtcggctgg actcacatcg gttactcggt cggcgtgaag gctattgata aagtgcgaga
    18781 gaactcgcag atgattgccc gttcggcaac catcctcggg atcacggtaa tcggcgggct
    18841 gatcgcttcg tatgtgcata ttaacgtggt gacatcgttt gccatcgaca gtacccacag
    18901 cgtcgcgctg cagcaggatt tcttcgataa agtcttcccg aacattttac cgatggccta
    18961 caccctgctg atgtattact tcctgcgggt gaaaaaagcg catccggtgc tgttaatcgg
    19021 cgtgacgttt gttctttcta ttgtttgttc tgcattcggc attttgtaaa tggaacgagg
    19081 caccgcgtct ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa cccttcaaca
    19141 agttgaaaac tatacggcgt taagtgaacg tgccagcgaa tatttattgg ccgtgatccg
    19201 tagcaaaccg gatgccgtga tttgcctggc gaccggagcc acgccattac tgacgtatca
    19261 ttatctggta gaaaaaatcc accagcagca ggttgatgtc agccagctca ccttcgtgaa
    19321 gctcgacgaa tgggtggatc tgccattaac gatgcctggt acctgcgaaa ctttcctgca
    19381 acagcatatc gtgcagccgc tggggctacg tgaagaccag ctcatcagct ttcgctccga
    19441 agagattaat gagacagagt gcgaacgggt aacgaacctg attgcgcgca aaggcggtct
    19501 ggatttatgc gttctcgggt tggggaaaaa cggtcatctt gggctgaacg aaccgggaga
    19561 aagcctgcaa ccggcctgcc atatcagtca acttgatgcc agaacacagc aacatgagat
    19621 gttaaaaacc gcgggccgtc ctgtgactcg tgggatcacc ttaggcctga aggatattct
    19681 caatgcccgc gaagttttgt tactggtgac tggcgaagga aagcaggatg cgacagaacg
    19741 ttttctcacg gctaaagtct ctaccgctat cccggcttca tttttatggc tgcataacta
    19801 tttcacatgc atgattgatg agatgtgtcg tcgttaactc gcctgctaca ggcgttgcaa
    19861 cgctattcct gctgcgacgc ctgtaatcac tctccctgct gctccagcgc atacttatac
    19921 agcgcatttt tcttcacgcc gtgaatctct gccgccagcg ccgccgcttt tttcagcggc
    19981 agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgc
    20041 gctttatggc cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttctttg
    20101 acccacgcca gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc
    20161 tcacgcgcca gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta
    20221 tctaacagac ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatagct
    20281 tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcata acaaaaacga
    20341 tcggatggta aacccgcggc gcttaacgca gtgatagcag cacacggccc tggtagcggc
    20401 accacgcgaa tccccgcttc acgacaggta cgcaccagat ggtagccagg atcgttaatt
    20461 agcggcgttc cggcatcgga aaccagcgca atgttttgcc cctcttgcag tttcgccagc
    20521 agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc
    20581 ccaaaatgtt gcagcaataa accggtgtga cgcgtatcct cggcggcaat cagatcaacg
    20641 gcctgtaata cctctaatgc acgctgggtg atatcagcca gattgccgat tggcgtcggt
    20701 acaatgtaaa gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc
    20761 gtattgccga tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta
    20821 ccctcaacat tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg
    20881 attttcgccg gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg
    20941 gcgcaggctg attctgcctt ttacctgcaa cagatgcagc aaagttctga tgataccagg
    21001 atcaactggc aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cggacaggcg
    21061 gtggagttgt ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca
    21121 ctgctggcgg cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg
    21181 gcgaaaatca cacctgccga tttagaacaa aaccagcaag cccgttactg gcaggcaaaa
    21241 atcgatgcca gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa
    21301 ccgctgctcg gtgcgaaaga aaagcagcag aatattgacg ccacatggca ggcgctctcc
    21361 tccatgactc aggagcaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa
    21421 ggctggctgg atttgcagcg cgtctggttt gataaccgta acgatcctga catgatgaaa
    21481 gctgggatcg ccgactggca gaaacgttat ccgaacaacc cgggcgcgaa aatgctacca
    21541 acgcagttgg ttaacgtaaa agcgtttaaa cccgcatcga ccaacaaaat cgccctgctg
    21601 ttgccgctga atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcg
    21661 gcgaaaaata tcggcactca gccagtggcg gcccaggtag cagccgcacc tgccgcagac
    21721 gtagcagaac aacctcagcc gcaaaccgtg gatggcgttg ccagcccggc acaagcttcg
    21781 gttagcgagc tgaccggtga acagcctgcg gcccagccgg tacctgtaag cgccccggcg
    21841 acaagcaccg cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat
    21901 acctcatcac aaccacttag ccagatctta agccaggttc agcaggatgg tgcgagtatt
    21961 gtggtcggtc cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg
    22021 aacgtgctgg cactgaacca gccggagaac atcgaaaacc gcgtcaatat ttgttacttc
    22081 gcgctttcac cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa
    22141 caagcgccgc tggtgctgat cccacgcagt tcattgggcg accgcgtagc caatgcgttt
    22201 gcgcaagagt ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc
    22261 agcgaattac gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt
    22321 actcccagag cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg
    22381 ccaaccgatg accagttcac caataatggc ggtcgtgtcg atgcggtgta cattgtggca
    22441 acgccgggtg aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc
    22501 ggtgcaacgc tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccgt
    22561 ctggagatgg aaggcttgca gtatagcgaa atcccgatgc tggcaggcgg taatctgccg
    22621 ttaatgcagc aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg
    22681 atgggcgtcg atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt
    22741 tttgaaatca acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg
    22801 aagttatcat ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac
    22861 agaccggcga tgtgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt
    22921 ttatcgccgc taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc
    22981 ggatcaccgt ttttgtcgag gtgcgttacc gccgctctgc gctttatggc ggcgcggcag
    23041 ccagtgtgac ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc
    23101 gtcataatgg gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga
    23161 atgaggttga gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt
    23221 agcgtgcaag aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg
    23281 gcagaggcgc ttccggatgc catctcgcgt gcagccatga cgctggttca gtctctgctc
    23341 aatggcaaca aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt
    23401 gctgccagca tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta
    23461 aatactgata atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat
    23521 gcaaaacagg tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt
    23581 ggcaacagcc gcgatattgt aaaagcggta gaagctgccg ttacgcgtga tatgaccatt
    23641 gtggcattaa ccggctatga cggcggtgaa cttgcaggtt tgttagggcc acaggatgtg
    23701 gagatccgca ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta
    23761 aattgcctgt gcgatctgat cgataacacg cttttccctc accaggatgt ttaaggagaa
    23821 tacatgaagg cattatcgcc aatcgcagtc cttctttccg cgctgctgtt gcaaggttgt
    23881 gttgccgctg ccgtagtggg taccgctgct gtaggtacca aagccgcaac tgacccacgc
    23941 agtgtcggca cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg
    24001 aaagacgaac aaattaagaa agaagcgcgc attaatgtca cggcctatca gggcaaagtg
    24061 ctgctggttg ggcagtcacc aaacgctgaa ctttcggctc gcgccaaaca gattgctatg
    24121 ggcgtagacg gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg
    24181 ggcgaagcgt ctaacgatac gtggatcacc accaaagtac gttcgcagct gttaaccagc
    24241 gacctggtga aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg
    24301 gggctggtga ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtaagcggc
    24361 gtgaagcggg taactacggc gtttacgttt attaaatagc agcccttgta atgcctgatg
    24421 cgacgcttgc agcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt
    24481 ttacgccgca tccgacatgt tttccctcaa atcaaaccaa cagcgccagc ccgccgacaa
    24541 tcacaccaga cactgccacc atcgcccccg tcagccataa ggctttcgcc gggaacgctt
    24601 tgcgcagcat aatcagtgac ggcaaactca ccgccgggag cgtcatcaac aatgccagcg
    24661 ccggagcagt tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg
    24721 tgggaatgac aaacaagcat cccgctaccg ccatcgccac cacccacatc aggctgttat
    24781 cgacagcacc atcggcatgg gggaataacc agacgcgagc ggcacccaac accagtactg
    24841 caaggatgta aaccgggatc gtactccaga aaagcgtcca tagcgccctg ccccagcggc
    24901 taaaaaatcc tccctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg
    24961 tttcacgcac ccatttttgc accagcgtcg caatcagcaa caccatcacc agccccgcca
    25021 ccagacgaat cgccgcaaaa ccccagccga ggacgaaccc cataaacacc agcgtcgccg
    25081 ggtttaacaa cggattcccc atccagaatg ccagcgcacc gcccatcgac acctgttggc
    25141 gacgcattcc cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa
    25201 acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aataaccagt
    25261 cacgcgggat cagcacctga atcaacgacc cgagcagtac gcccagcacc gccgctttcc
    25321 ataccgcgag gaaatagatc atcgcgtaat ccaacgccgc ctgccacgga ttagcatccg
    25381 cctgcgcaag gatagattta ccgatactgt gggtttcggc agcagtaaac gctttgccgt
    25441 agtaaggttg ccatttcaca taccagagac cagcaatgac aacgagaaag aaaagcgcgg
    25501 gtttccacca ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc
    25561 ccaggaataa gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga
    25621 ataattattc agctcttttg cgtaattctg aagagcttaa aatcgtcaca ccttcatgct
    25681 caggccgcat cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc
    25741 agttacccgg taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc
    25801 tacgatcgcc cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcga
    25861 ttaatgcttc ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattag
    25921 cacccatagc actgaccacc agcatatgct gcgcaccgag ccgccgcccg gttaatgcgg
    25981 tatccaccac cagcgtgtaa tcggcatgaa taaacgcctc tttactcccc gcttctcgcc
    26041 gcgtggtgcc gagacaacaa aacacaatgt cgatgggatc ggtgacctgc gccagcgcgt
    26101 cggtcagttg cggatcatgg ggattaaaca caccgggcat atcgcccaac ggacgtcgcg
    26161 tcggcgcggc tatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccac
    26221 ccaccagccc cgttgcgcct gtaatcagta cctggctcat cttcgctcct ttacagaatt
    26281 gtctgccttg cgctccccgg ctcatgcacc aggcttaata gtctacgagg taattatccc
    26341 cgattgtggg aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa
    26401 ttgccgtttt aatcactgat gaatttgagg attcagaatt tacttcaccc gcagacgagt
    26461 tccgtaaagc cggacacgaa gtgattacca ttgaaaaaca agcgggtaaa acggtgaaag
    26521 gcaaaaaagg agaagccagc gtggccatcg ataaatccat cgatgaagtg acgccagcgg
    26581 agtttgatgc cctgctgcta ccgggcggcc attcaccgga ttatctgcgt ggcgacaacc
    26641 gttttgtcac tttcacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc
    26701 acggtccgca gctgctgatc agcgccgatg tgattcgggg gcgcaaactg actgccgtta
    26761 aaccgatcat tattgatgtt aaaaacgcgg gcgcggaatt ttacgatcag gaagtggtgg
    26821 tcgataaaga tcagctggtt accagccgta caccggacga tctgccagcg tttaaccgcg
    26881 aggcattacg cctgctcggt gcctgagtta cgtagccaga tcattttttt accaaagccc
    26941 agcgtgttgt cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc
    27001 attctggcaa ccggaaagcg gcgattgtac gcctggcgcg caaggtcgct ttcctcacct
    27061 tcaagcctgc ggatctcgcc tttaaattgc acgccgcgaa ttaacgctac cgttttcggc
    27121 tgaccgttta ccgttccggc aactgccgcc tgcggcccac tcatctgcgc gtggcgcgtt
    27181 ttttcttccg tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta
    27241 gcgcaccaga gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc
    27301 caacggctga tggcggtgag tgtttccatt gctgttctct tttatactgt gggcctgacc
    27361 ttaacatgcg acgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacga
    27421 taagctttat accgggatca ccacagatgt cgaacgccgc tatcagcagc accaaagcgg
    27481 caaaggggcg aaagcactgc gcgggaaagg agaactaacg ctggcgtttt ccgcgccagt
    27541 gggcgatcgt tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca
    27601 aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac
    27661 cccaaagatt aaaagcgatt gaagtgctcg tgatactcaa ccagaccagt tacaccgttc
    27721 agcgcgtcat ccgctaaacg atgtacctgg aaggcacttt cagtgcccgg ccagcggcaa
    27781 cgcagatcgt gatgtgccgc cagttcaaag ccgaaacggc tgtacagcgc cggatcgccc
    27841 agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt
    27901 tggcgtgcca gcccctgtcc acggtatttt tcatctaccg ccagcggtgc catgccgacc
    27961 cattgcaggt cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga
    28021 ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga
    28081 accagcttcg cttccgcatc actttcgaat gaacgacgca gcagggcatc aatacccggc
    28141 gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt
    28201 caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta
    28261 aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca tacagcccca
    28321 gctccgtatc gccttcaatt accagccgac gctggaagaa gagcgtatcc ggatcttgtt
    28381 tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg
    28441 cgttctggct aacgaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat
    28501 cacgcacatg aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccaacg
    28561 cctggcggaa ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg
    28621 tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt
    28681 tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa
    28741 ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact
    28801 ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac
    28861 atttttgcgc tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg
    28921 gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc
    28981 taaaagatga taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc
    29041 aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt
    29101 ttgcgcatcc ggacggttac gcccgctggc agcgcgccgt ggatatggcg gcgcagttgg
    29161 gtgccgacgc gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc
    29221 cgcatattga gcgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact
    29281 tttatcatcg ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc
    29341 aggtgaaaca actggcacgg gtcacacctg tcccgctgga agtgtttgct ttcggcagcc
    29401 tgtgcattat gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca
    29461 acaccgtggg cgcgtgttct ccggcccgtt tcgtgcgctg gcagcaaacg ccgcagggac
    29521 tggaatctcg cctgaacgaa gtgctgatcg accgttatca ggatggcgaa aacgcaggtt
    29581 atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag
    29641 aacccaccag tctcaatacc ctggaactgc tgccggagtt aatggcggcg aatattgctt
    29701 cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct
    29761 ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt
    29821 ggatggagac gctcgggtcg atgtccgaag gcacgcaaac tacccttggc gcgtatcacc
    29881 gtaaatggca gtgagaaaag caatgaaata ttccttaggg ccggtgctat ggtactggcc
    29941 aaaagagacg ctggaagact tttatcagca ggccgccgcc agcagcgcag acgtgattta
    30001 tcttggtgaa gcggtctgta gcaaacgccg ggcaaccaaa gttggcgact ggctggagat
    30061 ggcaaaatcg ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca
    30121 ggcgtcctcc gaacttggcg aactgaaacg ttatgttgag aacggtgagt ttctgattga
    30181 agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg
    30241 gcacgcgctg aactgctaca acgcggtgac actgaaaata ttgctcaaac agggcatgat
    30301 gcgctggtgt atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg
    30361 cgatgagctg ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctgcc
    30421 gctggcctac tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg
    30481 tgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg ctatcgcagg aaaaccaaca
    30541 agtgtttgta ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga
    30601 gctggcatcc atgcaggggc tggtcgatgt ggtccgcctg tcaccgcagg gtactgacac
    30661 tttcgcgata ctcgacgcct tccgcgccaa tgaaaatggc gctgcaccac tgccgttgac
    30721 ggcaaacagc gattgtaacg gctactggcg gcggctggcc gggctggagc tgcaagccta
    30781 agtaaatagc tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa
    30841 tcagtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg
    30901 gcccccattc ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgacctcgcc
    30961 cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cggaacacca caatatgacc
    31021 ggcattgcca gtgctgcaac atcagttttg attggctatc tggcggcgaa taccactacg
    31081 ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccattggt gatcgccgaa
    31141 cagttcggca cgcttaatac gctctatccg gggcgaatcg atttggggct gggtcgtgcg
    31201 ccgggtagtg accagcgaac catgatggca ctacgtcgtc atatgagcgg tgatattgat
    31261 aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg
    31321 catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc
    31381 ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt ttgcgtttgc ctcacacttc
    31441 gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa accgtcggca
    31501 cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgctgccga cagcaaccgc
    31561 gacgctgaat ttctgtttac ctcaatgcag caagcctttg tgaaactacg ccgtggcgaa
    31621 accggacaac taccgccacc gattcaaaat atggatcagt tctggtcacc gtctgagcag
    31681 tatggcgtgc agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa agtgcgtcat
    31741 ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggttaa cgggcagatt
    31801 ttcgaccacc aggcacgcct gcattcgttt gagctggcga tggatgtgaa ggaagagttg
    31861 ttggggtagt gtgttttaac gtaggaagcc ttatccgaga tggcaacgct gtcctacgta
    31921 ggcctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac
    31981 cgctgattac tgatacaccg gcagcaaatt aaagctcgat aagatatgga ccagtgcgtt
    32041 gccaacgcca aataccagaa tcagcataat catcggcttg ccgccccaga cgcggaattt
    32101 cgggctacca aagcgtttac gcgatgcgcg ggccaacagc gccggaacaa ttgccgccca
    32161 gatggtagcc gctaagccag cataaccaat ggcgtacagg aatccgttag gaaacagcag
    32221 gccacccaca acaggcgggg caaaggtcag caacgctgtt ttcaaacggc ccatagccga
    32281 gtcgtcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa
    32341 cgaactcgct accgcaaagt ttgagaacac gaccagcaga agatccaggc tacggctgtt
    32401 cagcacgccg cttaatgcct gtaccagcac atcaatatta ccgcccttct cggcgatgcc
    32461 gataaactcc ggacgaggga tgttgcccat cgtcgccagc aaccagatgg tatacagcgc
    32521 cagcgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta
    32581 atacttcatc aggctcggca cgttaccgtg ataaccaaac gatgccagac agaatggcag
    32641 tgtcatcagc agatacggcg cataagacgc attgctttcg gcgacgttga acaaggtcgt
    32701 tggctgtaca tgccccagca ggctgccgaa ggtgaggaag aaagtaatga ctttcgcccc
    32761 cagcacaatt gctgtcatcc ggctgacagc tttcgtactc aaccacacca cgaacgctac
    32821 cagcagtgca aaaccaaatc cagctgcccg tgcagggacg ttcagcgaca tctccgcgaa
    32881 ggtgtgatgc agaatcgaac cgctggcaga aatataggcg taagtcagga tatagagcac
    32941 aaaggcaatg gaaatgccgt tgactacgtt ccagcctttg cccagcaaat ctttggtgat
    33001 ggtgtcaaag ctagaaccga ttcgataatt caggttagct tccagaatca tcaagccgga
    33061 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaga accacgcccc
    33121 ggacatgacc acaggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac
    33181 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    33241 tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacacgagt
    33301 acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat tattttacag
    33361 attgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga
    33421 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    33481 acgaccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccact
    33541 gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa
    33601 ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc
    33661 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccaat
    33721 gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac
    33781 accatcatcg cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc
    33841 acgacgcgga cgatcttcac ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc
    33901 acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat
    33961 cagagtacgt tcaccctgtg ccattttcag cagtgcggcg gccagagttt cgagatccag
    34021 ctcttcacct tctgccgtcg gctgaatttt gctcagcagt gcacggtatt gatccagatc
    34081 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacgac gtttgcccag
    34141 cagttctgcg ttcggcagtt caacttccgg aatggtcagc ttcatggtgc gttcaatgtt
    34201 acgcagcaga cgacgctcac ggttctcaac gaacagcagc gcacggccag cacggcccgc
    34261 acgaccagta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    34321 aactaccagg ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag
    34381 gatgtccaga cgaccatctt tcagtcgttc cagtgtctgc tcacgcagcg cctggttcat
    34441 gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agggcttctg ccacttccag
    34501 agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    34561 acgtaccagt gcttcgtttt tacgcatacc ccagacagtc cagtagctct ggctgatgtc
    34621 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    34681 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    34741 cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    34801 agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg
    34861 gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    34921 ttgcacgtca taacgttggc cgccgtacag ggcaaccacg tttacgccgc gcatgtgttt
    34981 agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac
    35041 cagaatctgt ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc
    35101 tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg
    35161 tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    35221 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    35281 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    35341 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    35401 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    35461 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    35521 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    35581 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    35641 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    35701 tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc
    35761 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    35821 gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    35881 agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag
    35941 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    36001 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc
    36061 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    36121 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    36181 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    36241 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    36301 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    36361 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    36421 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    36481 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    36541 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    36601 ttctcaacgc gtttgtcagc gatctgagag atgtgtacca gaccttcttt accgccgccg
    36661 atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccattgta gacgcggccc
    36721 acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg
    36781 tcggtcgctg cgattttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct
    36841 tcggtcagag cacggataac agaaccgcct ttaccgataa cgtctttgat cttgtccggg
    36901 ttgatcttga tggtatggat acgcggagcg aactcagaga tatcgccgcg cggcgcgttg
    36961 atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca
    37021 acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata
    37081 ccgtcacggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    37141 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    37201 acggcagctt tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa
    37261 gccatagaag aggaaccgtt ggattcggtg atttcagaca caacacgtac ggtatacggg
    37321 aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca
    37381 atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacagagta cggagggaag
    37441 ttgtagtgga acaggaaggt gtcagtacgt tcgcccatca gttcatcaag aacctgcgcg
    37501 tcacgtgcag tacctagcgt tgcggtaacc agcgcctgag tttcaccacg ggtgaacagc
    37561 gcagaaccgt gagtacgcgg cagcacacca gtacgcacat ccagaccacg gatcatatct
    37621 ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacatttttc
    37681 tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc
    37741 agtgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    37801 gtgatgcggt aagcatcgct cagacgagct tcagccagtg cagcaacgcg cgcgttcagc
    37861 gcttcgttta ccggctccgg ctgccagtcc caacgcggtt taccggcttc tttcaccagt
    37921 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaagactac tgcgcccagc
    37981 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta
    38041 cccgcaacaa ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg
    38101 tactggtcat tgatgtaacc tacacgggca gcaccaatcg ggccattgaa cggaatacca
    38161 gacagagaca gggctgcgga agcaccaatc atcgcgacga tatccgggtt aacctgcggg
    38221 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    38281 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct
    38341 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    38401 acggtcagcg ggaagaagtc ctgacctggt ttggcttttt tctgaccaac aacggtaacg
    38461 aataccgcag tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg
    38521 ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc
    38581 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    38641 cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc
    38701 tcattagccg cgcgaacctc tgcagcggaa gatcattcat agcaacaata cattagtttc
    38761 cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact
    38821 cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct
    38881 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    38941 cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca
    39001 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    39061 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    39121 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    39181 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacttgttat cgcaagacgg
    39241 ttaacattac gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc
    39301 aatttcgccc ataccgataa acttgccgtt ctcaccctct gtaacgcgaa ccagtccttc
    39361 cagcggcgca ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa
    39421 cggaagattc accactggat agtccgaagc tggactgtcc attggcatca gtaatggatc
    39481 aagtaactcc gcagccggaa tatcctgctg ttcggcttgc tcaaccagtt cacgcagatg
    39541 ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaagtaaat
    39601 aacatgcgcg ccacaaccga gtttttcgcc gagatcgtca atgatagtgc ggatataagt
    39661 gccttttgag cagtgaattt ccagctccag ctcattgcct tcatggcgaa taaacagcaa
    39721 ttcataaacg gtaatcgggc gcgcttcacg cggaacttca atgccctgac gcgcgtattc
    39781 gtacagtttt ttgccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc
    39841 gccacggaag gtatccagcg ccgccgccag ttgctctgca ctaaaggtta ccggacgttc
    39901 ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac
    39961 ccgataacgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca
    40021 aattggcaac atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc
    40081 gttataaatc cgtttcactt tttgcagcgc atcgttgctg gacatcccct gcggtttatc
    40141 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    40201 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    40261 tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg
    40321 atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcctgcagc
    40381 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt tacatatact
    40441 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccaga
    40501 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    40561 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct
    40621 gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga
    40681 cgcgccagcg tcgcatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat
    40741 ggaaccctaa aaaccttaag cgatggtacg ttggatttcg atgatttcga atacttcgat
    40801 cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt
    40861 acggacttcg ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat
    40921 aaccacgttg tcgcgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac
    40981 catacaacct gcgatggcac caaatttcgg tgatttgaat acgtcacgaa cttccgccag
    41041 accgataatc tgttgtttca gttccggaga cagcataccg ctcattgccg ctttcacttc
    41101 gtcgatcagg ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac
    41161 tttacgtgca gaggcatcag cacgtacgtt gaagccaacc aggatggcat tggacgccgc
    41221 agccagggta gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt
    41281 aacttcgtca gtagacagtt tcagcaagga gtcggagata gcttccacag aaccctgtac
    41341 gtctgccttc aggacgatat tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat
    41401 gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg
    41461 atagagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc
    41521 agccggaacg ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac
    41581 ttcctgaccc agttcgttgc gcatcgcacg aacacgaccg tattcgaagc cacacagaac
    41641 gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc
    41701 tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag
    41761 ttccagaact tccgcctgca gcaggatagc gtccagcagc tcatcgatac cagtacccgc
    41821 tttcgcagac acatgtacga actggctttc accgccccac tcttccggca ggatgccgta
    41881 ctgggagagt tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac
    41941 tgcaaccacc accggaacct gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg
    42001 cattacgccg tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg
    42061 agcacgcatt gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt
    42121 ttcagtttca acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc
    42181 cactttcgtt gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat
    42241 gatggtcaca accggcgcgc gcggttcagc cgctgcaccc gtgtcacggt cgctcattac
    42301 cgcttcttcc aactcgtttt cacgacgcag gataactttg tggcccatct cttcagcaac
    42361 cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat
    42421 catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt
    42481 gatggtttcg ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg
    42541 ctgcagcgaa gaacctttac gttttccgcc cttaccgcca cgtactgctg cgcgtgcttc
    42601 ttcacgatca gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg cagctttcgc
    42661 gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg
    42721 agcatgttga gaagtagtaa cgtgataatc gctggaatct tcagtcggtt ccgcgttatc
    42781 agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc
    42841 ttcgagttta cgacgcgctt cttcttcggc tttacgcttg agctctgcag cttcctgctc
    42901 acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt
    42961 gtctttttcc gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc
    43021 ggcctcacgt tcagcttttt gttgcgcctc acgtttggcc gattcttctg cctcacgacg
    43081 ggcttgctct tccgcttcac gctgcgcttg ctcttccgca gcaaggcgtt cagcctcttg
    43141 cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt
    43201 tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc
    43261 cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac
    43321 agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac
    43381 ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc
    43441 tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag
    43501 tgctccggct ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa tgccctgttc
    43561 ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc
    43621 tatcccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg
    43681 ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg
    43741 ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag
    43801 cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt
    43861 ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac
    43921 ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccatt
    43981 acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc
    44041 ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca cgaactgcgc
    44101 cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc
    44161 ctgaacacgc gcgccacgca tacctacgca agcacctacc ggatcgatac gtttatcgtt
    44221 ggttttcacc gcgattttcg cacgagaacc cggatcgcga gccgctgctt taatttcaat
    44281 cacttcttcg ccgatttccg gcacttcaat gcggaacagt tcgatcagca tttccggctt
    44341 ggaacgggtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat agagcacgcc
    44401 acgaacgcgg tcgccaggac ggaagttttc acgcggcagc atatcttcgc gcaggatcac
    44461 ggcttcagcg ttgttgccca gatccagaga gatgttgtcg cggtttactt ttttcaccac
    44521 gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc
    44581 ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtggtgatac ggtcaaaggt
    44641 aacagactca atctgatctt caacgtaatc gcccaggttc aggctttcat cttcataacg
    44701 tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg
    44761 acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acacggacgt cgatctcttg
    44821 ctcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc
    44881 gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat
    44941 cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat
    45001 attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc
    45061 taccgctttg ataacgccct gccatttacg acggttttgt accgccatgc ggagaaccag
    45121 agtcacctct tctccgacga aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag
    45181 acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac
    45241 agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc
    45301 aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa
    45361 acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa
    45421 gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca
    45481 aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg
    45541 gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat
    45601 atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt
    45661 cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa cgtggcaaat
    45721 tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc gagacgggac
    45781 gtaaaatctg caggcattat agtgatccac gccacatttt gtcaacgttt attgctaatc
    45841 acgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc tcctgctctc
    45901 ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg ataaaagatg
    45961 attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctcc
    46021 tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta
    46081 ggtcaacgta ttggtatcgc tttttccggc ggtctggaca ccagtgccgc actgctgtgg
    46141 atgcgacaaa agggagctgt tccttatgca tatactgcaa atctgggcca gccagacgaa
    46201 gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg
    46261 atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt
    46321 cataacacca ctggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact
    46381 ggcaccatgc tggttgctgc tatgaaagaa gatggcgtga atatctgggg tgacggtagc
    46441 acctataaag gaaacgatat cgaacgtttc tatcgttacg gtctgctgac caatgctgaa
    46501 ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg cggtcgtcat
    46561 gaaatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc
    46621 tactccacgg actccaacat gctcggcgca acgcatgaag cgaaggatct ggaatacctc
    46681 aactccagcg tcaaaatcgt caacccgatt atgggcgtga agttctggga tgagagcgtg
    46741 aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt ggcgctgaac
    46801 ggtaaaacct ttagcgacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt
    46861 cacggcctgg gcatgagcga ccagattgaa aaccgtatca tcgaagcaaa aagccgcggc
    46921 atttatgaag cccctgggat ggcactgctg cacattgcgt atgaacgttt gttgaccggt
    46981 attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt gggccgtctg
    47041 ctgtaccagg gacgttggtt tgactcccag gcgctgatgc tgcgggactc tctgcaacgc
    47101 tgggttgcca gccagatcac tggtgaagtt actctggagc tgcgccgcgg gaacgattat
    47161 tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa
    47221 aaaggcgact cggtgttctc gccagatgat cgtatcggtc aattgaccat gcgtaacctg
    47281 gatatcaccg atacccgcga gaaacttttc ggttatgcca aaactggcct cctctcctcc
    47341 tctgccgctt caggcgtgcc gcaggtggag aatctggaaa ataaaggcca gtaattcgac
    47401 atcaaccctg cccttcaggg gtggggttac cttgcttatc ctctgtaaac tcctttctga
    47461 gcatcacata atcgaaaaca tcatggtttg catccacaac ccgtgagtct cctttatagt
    47521 gtgcaatcac ttcctccagc gttttcggtt gttccggctt tgttaccccc atccacgcat
    47581 taatcaggta attattgtaa gctgtggaaa agatgttgtt ttccgttgtg cgatccacgc
    47641 catccccaag aacggggcta taccagataa acatcgggac atgatatgcc tgctggctgg
    47701 cctccctgcc tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc aggccgtggt
    47761 cggcaaaata catgaccgag gcgcggcgat cttttaataa ttcaaaaacc tgacctagca
    47821 aactatctgt gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa
    47881 acacggcgct ggattgcgga taggcgctac aagccggttc atggcttcca tttaaatgaa
    47941 gaacaatcag tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca
    48001 acaattcatc aaatcctctg acataaactg tttccatggc gcgcatggcg atactggtaa
    48061 ctgctgtacc attctgccga aaagcggatt gcgagcttag ccagaaagtc tgaaatcctg
    48121 cttgattagc catattaata atgttgtccg ggtaattatg aatgtcatga ctcaaaacgg
    48181 aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta aggtgcaccg cttattgctt
    48241 gattaaacag tttgatctgt tttctttgtg cttcgacttg cggtgtcgta gagcgtgtat
    48301 atccatacaa agacatattg tcgacgcgta cagactcccc cacaatcaac acgtaggtat
    48361 caatacctgt atccctgact gataattgaa aatacggcac cgtgtttgca attgagagta
    48421 atctttgatg ctctttcgct gctaaagcaa aataattgag attgaaaaac ggtgtatagg
    48481 tagcaaatcg cgacgctagt atatatggac tgaacgcttt tttatttttt gcatctttat
    48541 aagcaaattg acaagcggaa aataaactgc ccgaaatgac aatcagcaat aatattcctg
    48601 tcactttttt cgtcggcaag gaaacatcat attttattat tactgccaaa aaaagaaggg
    48661 ataaaaaggc aaaagcacat agataaggga tatacatccc cagcattttg acaacttcat
    48721 ccggatcact ctgcagcaca ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac
    48781 accatgaata gaggctaata ctcatgtctg ccgctgtcag taacacaaat ggtgcggcaa
    48841 caagcaggcg aagcggaagg gatgatatac gtttaacaac caggatggtc agaacgaaga
    48901 acagcaatcg ctgaaccata tgcgctccgg aagaggcgac taagttcgta ataccaaaaa
    48961 caataacaca aagatacaac aaccaatgag atttaaaact tctaacaaat ttgttgaata
    49021 ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa tgttcaattt
    49081 tatatcagac atgtttttca actctgatcc atatcagacc gaaacactgt tagttttttc
    49141 tttttaaatg atgcggccat tgttctcagt aattaaataa cattatcaat gcgtctctaa
    49201 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    49261 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    49321 tcagcagaag ggaaaagttt ataactaagg cgtaccgata ccatcgtttc aaagtaccag
    49381 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    49441 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    49501 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    49561 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    49621 tgctggctga atttgttcgg ttttcgccgg cgcactcagg ttttcccatt cgctaccttt
    49681 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    49741 cagcagcgct gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    49801 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    49861 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    49921 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    49981 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    50041 aattttattg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    50101 aaaaaggcga caatcgccgc ctttttagcc agttatttat cgttttaaac ggcttttact
    50161 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    50221 acgcgaatta acggttcggt gccggattta cgcaacagca cacgtccacg gctacccagc
    50281 gcagcttcaa cttctgcggt cacagctttg actgactcat gctccagcgg atcgccgctg
    50341 cctgctgtgt aacgcacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    50401 tcgtgcaggc tcatatggtt acgcgccatc gccgccagca cctgcaagcc agcaacaatg
    50461 ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggagttctc tgcaccgata
    50521 cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
    50581 aatggaatac ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    50641 acagcaccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    50701 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg
    50761 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    50821 tcggtagccc ccacttcggc attgatgttt acaccgttcg gctcacaacc gatagcgata
    50881 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    50941 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    51001 aactcgatat agcgacccgc agcatcaacg atacggctgg ctttacccag ttctgccgaa
    51061 tcaacgcagc tgatctcttt ttccatttcc gcttcgatgg cctcttctac cgcatccggt
    51121 agtttggtgc cgtcgataga gaagaattta atgccgttat cgtagaatgg gttatgcgat
    51181 gcagaaatta caattccagc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    51241 gttggcatcg ggccagtgaa gagcgcggaa agaccggctg ccgcaagccc cgcttccagt
    51301 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    51361 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggggtg
    51421 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    51481 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    51541 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    51601 ggccagacta ccgctcaaac gctcggacgg ccccacgttc agcagctgtc caatcatcga
    51661 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    51721 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    51781 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    51841 cacttctgca aagacatcgt gatacttcgg ggcttcctgc atggtttttg gatttccctg
    51901 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    51961 ggaaagggag cggatatcgt taataatgtg agcgccaact ttcgctgact cacggatgac
    52021 ttctggtttg gatgtatcta ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    52081 cacaggaata acacgttgta actcttcttc aacgctaact tccgccgccc ctggacgtgt
    52141 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcgttg atcatcagat tcgcatgttt
    52201 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    52261 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    52321 cataatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    52381 ttggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    52441 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    52501 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    52561 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    52621 tctttcatcg catgcagtat atccatattg tcggtcagaa gctgacgcgc acgattatag
    52681 ttgcgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    52741 tgtttcgcct tcgctacgct acggccgagg aacacttcac cttcttcttc cgcgtacagc
    52801 agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc
    52861 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct
    52921 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctagcg
    52981 ctgattgcgt cgccctcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    53041 atcgtcactt tgtgcaccgg atcatgttcc ggcaccaggc gaccgataat cgcatgaccc
    53101 gcttcgtggt aagccgtcga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    53161 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    53221 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    53281 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga
    53341 cgcatgtgaa ctttcaggat ctgttcacga ccgcgaacat ctggcaagcc aaccacaacc
    53401 tgacggtcga aacggccagg acgcagcagc gccgggtcga gaacgtccgg acggttagtc
    53461 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    53521 ttcagagtct gttcacgttc atcgtgacca ccgcccagac ctgcgccacg ctggcggcct
    53581 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    53641 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    53701 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    53761 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    53821 cggctcggct cgcgcagata ctcaaccagt tcagcaactt cttcttttgc ttcgtcgcag
    53881 cccgcaacgt cagcaaaggt cgttttgatc tgatcttcag tcagcatgcg cgctttgctc
    53941 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    54001 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    54061 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaagtt atccagtaat
    54121 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    54181 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    54241 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    54301 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    54361 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    54421 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    54481 tcccgcgaac gtgcgcgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    54541 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    54601 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    54661 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    54721 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    54781 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    54841 atacggcctt taccgccaat ttgggtgacc acatattgtg accaaccacc cggcgcagca
    54901 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    54961 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    55021 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    55081 ctttcccatt gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggc
    55141 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    55201 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    55261 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    55321 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    55381 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    55441 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    55501 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    55561 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    55621 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    55681 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    55741 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    55801 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    55861 accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    55921 cagcttggct tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc
    55981 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    56041 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    56101 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    56161 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    56221 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    56281 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    56341 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    56401 attgaccagc tgtatagcgt tcagtgttca ggccgcaaat gttgatgagt acattactca
    56461 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    56521 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    56581 actggcggcg ttgattcaac tcggccccga ttttcgtttt accacgacgc ttgaaaccaa
    56641 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    56701 gacgttaaga cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    56761 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    56821 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ccgccatagt
    56881 tgaccgcaac tgtttctccg tctcgctcta tagtgcccca aaacctggtg atatggcttt
    56941 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    57001 tggttctgcc gaagcgcaat actgcgagct ggatgtggtg ccaggcgacc tgaaccgctt
    57061 tacgctgacc ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    57121 ggatggtgcc agctatgctg gagcaattct gaaagatgag ttaaaacagg caggtatcac
    57181 ctggagcgga acactgctgc gtcagacgca ggttaacgaa cccggaacgg tagttgccag
    57241 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    57301 gattgccgat acggttttcc gcatgatcgg ccatgcgcgc ttcaatgtgc ctggaacatg
    57361 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    57421 aaataccatt attgccgatg gttcagggct ttcgcggcat aacctgattg ctcccgccac
    57481 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctctat
    57541 gctgccgctg gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    57601 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    57661 attcattacc acagcgagcg ggcaacgaat ggcgtttgtg caatatcttt ctggctatgc
    57721 agtagaaccg gcggatcagc gtaatcgccg tattccgttg gtacgttttg aaagccgttt
    57781 gtataaggat atttaccaga acaattagtc taaagagaaa ccccggcaca tggccggggt
    57841 tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg
    57901 tcatcccagt cttcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatca
    57961 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
    58021 tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc gctcgccgca
    58081 gagatcagat aatatttatc ttcccaaccc agcgcctcag cgatcgcttt cgctttctct
    58141 tcggcttctg ccttatccag caggtcgatc ttgttgaaga ctaaccaacg cggtttcgcc
    58201 gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga
    58261 tcggtgccat caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgctccagg
    58321 tgcttcagga agcgaatgcc cagacctgcg ccttctgcag cgccttcaat cagtcctgga
    58381 atatcggcaa caacgaagct cttttcgttg tccattcgta ccacaccaag acttggcacc
    58441 agagtggtaa acggataatc cgccactttc ggtttagccg ccgatactgc gcggataaat
    58501 gtcgatttac ccgcgtttgg catccccagc ataccgacgt cagccagcag catcagctcc
    58561 agtagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg tgtacggtta
    58621 actgacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
    58681 cgctgaccgt gtttggtcat atcgcccatg gtttcaccgg taccctggtc gataacacgc
    58741 gtacctaccg gcactttaat cgtcacgtct ttaccgcgct taccggtaca gtcgcggctt
    58801 gcgccattct gaccacgctc tgcgcggaaa gatttttcaa aacgataatc gataagcgtg
    58861 ttcaggttct cgtcggcttc catccatacg tcaccgccgt caccgccgtc gccgccatcc
    58921 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
    58981 cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc cgtaaatcat
    59041 tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga
    59101 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
    59161 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
    59221 aacagcgtaa acagtggggt gagcgtaatg atcgcgctca cctgcgctgc ctgccagcga
    59281 gccatcgctt ccgccagggc gccatatcct accaatgtat tcagtccgca aaaaattaaa
    59341 catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc
    59401 gcaattgtac ataaagtgta cagcaaaaac aggatctgcg gtgaggccag tcgacgcaat
    59461 aaaacctttt gcgccacgcc ataactcacc cacaccgtcg ccgcaccgac cccaaagatt
    59521 actccccagg tgtaatcggt gagctttgta aatatctcga ccagactggt gttaaaaaac
    59581 atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct
    59641 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc
    59701 gaagcggtcg gacccaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc
    59761 ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
    59821 ggcaaccgct tcttcaccgc aagaatggca cccaggccaa tactcgccat caagaaacgg
    59881 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac
    59941 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
    60001 ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc
    60061 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
    60121 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
    60181 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
    60241 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
    60301 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
    60361 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
    60421 cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac
    60481 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
    60541 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
    60601 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
    60661 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
    60721 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
    60781 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
    60841 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc
    60901 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
    60961 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
    61021 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt
    61081 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
    61141 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
    61201 tatccacacg gcgactctgc tgcacgacga cgttgtggat gaatcagata tgcgcagggg
    61261 taaagctacc gccaacgctg catttggcaa tgccgccagc gtgctggtag gcgattttat
    61321 ttatacccgc gcgttccaga tgatgaccag cctcggctcg ctcaaagtgc tggaagtcat
    61381 gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga
    61441 tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt
    61501 tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggagg aggagaaagg
    61561 cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
    61621 ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga acgaaggtaa
    61681 accgacgctg ccgctgctgc atgcaatgca tcatggcaca ccagaacagg cacagatgat
    61741 tcgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat
    61801 gaacgcttgt ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc
    61861 catcgcagcg ttacaggtgc tcccggacac cccgtggcga gaagcactca tcggcctcgc
    61921 gcacatcgct gttcaacgcg atcgttaatc ccctcccctc atcccgcgca gcgcgcggga
    61981 tgattccagt aaattccata aaaacactta ttcagctcta taacacctga aaaataggga
    62041 cgtacatctt tatatattct gaatattcac actctttaca ggaacttttt agagcaatag
    62101 gccatcagga gtatagtgat gctcgacaga agaagtgttc tgaatgaaag cgactaactt
    62161 aaggagtgag gaaagatgga aagtaatttc attgactggc atcccgctga catcattgcg
    62221 ggtttgcgca agaagggaac gtcaatggcg gcggaatctc gcagaaatgg tttgagttcc
    62281 tcaacgctgg cgaacgcatt atcgcgcccc tggccgaaag gagagatgat tattgcgaaa
    62341 gccctgggaa ctgacccctg gattatctgg ccatctcgct accatgatcc gcagacccat
    62401 gagtttatcg acagaacgca gttgatgcgt agctacacta aaccgaaaaa atgagtggtc
    62461 tggcggtagc cccgcgaacg gggctgccag ctctcagaca attactcgcc tttcacacgc
    62521 tcaatatttg cacctaaagc gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg
    62581 tgataaatac gatcaactac cgtcgtccct tccgcaatac agccagccag caccaggctt
    62641 gctgatgcac gcagatcggt tgccataacc tgtgcgccag aaagtttttc aacaccgtga
    62701 caaataacgg tattgctttc gatttcggcg tgcgcgccca tacggctcag ctctggcaca
    62761 tgcataaagc ggttttcaaa gaccgtttcg gtgatgaagc cggtcccttc tgccaccagg
    62821 ttcaacagcg tgaactgggc ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt
    62881 acgttaacag ccttcggacg tttgccatgc atatccaggc taatccagtc ttcgccgact
    62941 tcgatgtctg ctccagcgtc acgcagtttc gccagcacgg cgtcgagagt atctggctgc
    63001 gcgttacggc agataatttt gccgcgagag atcgccgccg ccaccaggaa agtaccggtt
    63061 tcgatacgat ccggcagcac gcgatagaca ccgccgccta aacgttccac accttcgatg
    63121 acgatacgat cggtgccctg accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg
    63181 gtatcgacga tttccggttc acgcgctgcg ttttcaataa tcgtggtgcc ttccgccagg
    63241 gttgcagcac acatgatggt caccgttgcg ccaacgctga ctttatccat cacgatatgt
    63301 gcgcctttca gacgaccatc gacggaagct ttaacgtaac cttcttccag tttgatggtc
    63361 gcgcctaatt gttcgagacc agaaatgtgt agatcaaccg gacgtgcacc aatcgtacaa
    63421 ccgccaggca gtgaaacttg cccctgacca aagcgcgcta ccagcggccc cagcgcccag
    63481 atagaagcac gcatggtttt aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg
    63541 gcatcaatat gcacagaacc attacgttct actttcgcac ccagctggct tagcagcttc
    63601 attgatgtat cgacgtcttt tagtttcggg acgttctgga tctctaccgg ttcttccgcc
    63661 agtagtgcgg caaaaaggat aggcagagca gcatttttag cgccggaaat tgtgacttcg
    63721 ccctggagct tcgttggccc ctgaacacga aatttatcca tttagtttgt tctcagttaa
    63781 caattcatat ccgctaccgg cgaatcgccc atagctcaaa agccgttcag tttgcgatcg
    63841 cgcgcccact ccgcaggggt atacgctttg atcgacacag catgaatgcg gttatccgca
    63901 atatattcca tcagcgggcc atagaccgtc tgctgttttt taacccgact catgccgtca
    63961 aacaactcac ccacggcaat aacctgaaag tggctgccat cgccggaaac gtggacttcc
    64021 tggagggaga gagcgttcat caacacgctc tgaatttcat tattttccat gggatcttca
    64081 atcatcagtt aataaaccag cgaaacatct tagagcaaag ttgcgctggc ataaataagc
    64141 aaaaagcctc gctgataaat cagacaaggc tcgacttgca ggcaggtttg ccggacaggc
    64201 ggttaacgcc atatccggcc tgaaaaaatt taacgaggca gaacatcagc aggcaaatta
    64261 tacaatttcg ccagggtata cactttgtcg tttaccccct gaagcgtcac attgttgccc
    64321 tgctttttcg ccagatcgat aagatggagc agcagcgcca gtccacctgt atcaacgcgg
    64381 gatacacggc taagatcgat acaggtaatc cccttcaccg cttcctcacg catttcccaa
    64441 agcggtagca gaacgtcctg atccagctct ccggataacg ccagcgtgtc acccgtctgc
    64501 atccagctca gtgactcgct cattattttt tctcttccag agtgattttc tgttgagaaa
    64561 tcgatttcag ttgcgcagtc aggccgtcaa tacctttggt acgcagcagc gttccccact
    64621 cgttttgttt ggtggtgatc atactgacgc cttcagcaat catgtcgtaa gcctgccagt
    64681 tgcccgtctg ggagttttta cgccactgga agtccagacg caccggcggg cggccattcg
    64741 ggtcaataat ggtaacgcga ataggcacaa tggttttatc acccagcggc tgttctggcg
    64801 caatctgata ggtttgaccg tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt
    64861 actcacggaa agcggcaaag taagcatcac gttgagctgg ggtcgcactc ttgtaatact
    64921 gacccagcac cagcgcaccg gcgtatttca cctgtacgta tggcagcagt tcctgatcaa
    64981 caatggtacg cagataatcc gggttggccc gaatttgtgg ttgctcattc ttcaggcgat
    65041 cgaacgtttt ctgcgccgcc tcgtccatca gcttatacgg attggtctgg tctgccgcgg
    65101 ttgccgcact cagcggtgca atcaccagca aagcgaccat cattaaacgt ttaaacatgc
    65161 gtcggttctc ctgaaattat ttcgttgtac ccacaggttc agtggtttca ttattacctg
    65221 gcgcagcagc tggcgcatcg ccactattct tattgtcatc gcctttacta ccgtaaagga
    65281 actgaccaat aagatcttcc agcaccatcg cagacttggt gtcctgaatt gtatcgccat
    65341 ccttcaggat agcagtcccc agttccgggt cttcaaaacc gacgtttaat gccagatatt
    65401 gttcccccag caggccggaa gtacgaatgc tcagcgaact ggtatcagga atgtggttat
    65461 aacgttgttc aatttccagc gttacgcgcg gcagataggt tttcgggtcc agcgtaatat
    65521 ccgccacccg gcccacaaca acaccaccaa tgctgaccgg agagcgcgct ttcaggccgc
    65581 caatgttatc gaacgtcgca taaagcgtgt aggtcggttc agtacgtatg gacgtcacgt
    65641 tcgccgcctt caggcaaaca aacagcgccg ccagtagtgc tgctaataaa aagataccca
    65701 cccaaatttc attttttttc gtttgcatga actcaattcc caaacatcaa tgcggtcagc
    65761 acaaaatcca gccccagaac agccagagac gagtggacaa cggtgcgagt ggttgcccgg
    65821 ctaatcccgg cagacgtcgg gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc
    65881 gtgatggcaa acaccacgct cttaatcaga cagttgacca gatccatacg ccagtcgacg
    65941 gcattttgca ttgccgacca gaagaacccg ctatcaatgc ctttccaact gacgccgacc
    66001 agagatccgc cccagatccc cacggcgacg aaaataaccg tcaacagtgg taatgaaata
    66061 accccagccc agaaacgggg agaaataacc cgacgcagcg gatccaccgc catcatctcc
    66121 atactggaga gttgctctgt agcgcgcatc aggccgattt ctgcggttag cgccgaacca
    66181 gcacgcccgg caaacaacaa cgcggcaacc accggcccca gttcacgcag tagcgataac
    66241 gccaccagca tacccagact ggtttccgca ctataagtgg tcagaaccag ataaccttgc
    66301 agccccaaca ccattccgat gaacacgcca gaaaccacaa taatcagcat cgacaggacg
    66361 ccgacattat agagctggcg caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg
    66421 accagcgcat tgaataacat taacccggcc cgcccgaacg ttctcagggt tttaatccct
    66481 ttatgtccga gcgacgccag cgcatttaac agcatgagtg gcttaactcc ctggtaaaag
    66541 atcagcgtga taatcgccgg caggatagcg gaacggaaca ggcccgtcag ctatcccgtc
    66601 cagaaactga cgtacgcgtg gatcaggatt cgcctgcaac gcctgggcac tgccatgagc
    66661 gacaattttt ttgtccgcca ggatccaggc gtgatcggca atacttaaca cttccggcac
    66721 atcgtgagaa accaccacac aagtcacacc cagcgcgctg ttcagctcag aaatcagctt
    66781 caccagtacg cccatggtga tgggatcttg cccaacaaag ggttcatcaa acatgatgag
    66841 atccggctcc agcgcaatcg cacgcgccag cgctgcacgc cgcgccatcc caccggaaag
    66901 ttcagaaggc attagtttag ccgctccacg cagccccacg gcctccagct tcatcatcac
    66961 cgtactatgc aacaatggcg cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt
    67021 gtcaaatacg ttcatatcag tgaacaacgc ccctgactga aataacatgc tcatccgttt
    67081 gcgcactgta tacaggcgcg aacgagacat cgccggaata ttctcaccat cgaaaagaat
    67141 ctcaccatga tctggtgcga tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat
    67201 gcccgatggc cccatgatcg ccgtgatctt ccctcgcggc acggtcaggg aaatattatc
    67261 gaagatgcag cgattgccac gcgtaaaact gacatcgcgc atatcgacta aattcgccac
    67321 agactgctcc ataattcacc cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt
    67381 aaccttgaac ccaacatatt tacagaatat tacccgccgt ggttagcgaa agctggcatt
    67441 tgttttactt tttagccgca taaagtcaaa attaagaatc cgttacggct ttctgaaaat
    67501 cttcagcgga ccggcgagta tacctgaaga aaggacgtta gatgctttta gctacggcac
    67561 tgttaattgt tggtttactt ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt
    67621 ctattctttg ccgaaccttt ggcatcccgc cgctaatcat cggcatgacg gtggtcagta
    67681 ttggtacatc gttaccagaa atcatcgtct cgcttgccgc gtctctgcac gaacaacgcg
    67741 atttagccgt tggtacagcc ctcggctcaa acattatcaa tatattgctg atcctcggtc
    67801 tggctgcgct ggttcgtcct tttaccgtcc attctgatgt tctacgccgt gaattaccct
    67861 taatgttgtt ggtcagcgtg gtggccggtt ccgtactcta tgacggacaa cttagtcgaa
    67921 gcgatggtat ctttctcctc tttctggctg tgctatggct gctgttcatt gttaaacttg
    67981 cacgtcaggc tgaacgtcag gggactgaca gcctgaccag agagcagctt gcagagctgc
    68041 ctcgtgacgg cggattgccc gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc
    68101 cagtggccac gcggatggtg gttgataacg ccacggtgct ggcgaattac tttgccatca
    68161 gcgagttgac gatgggtctg acggcaattg ctatcggaac cagcctgccg gaactggcaa
    68221 ccgcaatagc gggggttcgc aaaggtgaaa acgacattgc tgtcggaaat atcattggcg
    68281 caaacatttt taatattgtc atcgtattgg gtttacccgc gctgataacg ccaggagaga
    68341 ttgatccact ggcgtacagt cgtgactaca gcgtgatgtt gctggtgagc attatttttg
    68401 cgttgctgtg ctggcggcgc tccccgcaac cgggccgtgg tgtaggggta ttattaactg
    68461 gcggatttat cgtatggctg gcgatgttgt actggttatc gccaatactc gttgaataac
    68521 tggaaacgca ttatgtcgca cgtagagtta caaccgggtt ttgactttca gcaagcaggt
    68581 aaagaagtcc tggcgattga acgtgaatgc ctggcggagc ttgatcaata catcaatcag
    68641 aatttcacgc ttgcctgtga aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg
    68701 atggggaaat cggggcacat cgggcgcaaa atggccgcaa cgtttgccag caccggtaca
    68761 ccttcatttt tcgtccatcc tggtgaagcc gcgcatggtg atttaggcat ggtcacccca
    68821 caggatgtgg tgattgctat ctctaactca ggtgaatcca gcgaaatcac ggccttaatt
    68881 ccagtgctta agcgtcttca cgtaccgtta atctgcatca ccggtcgccc ggagagcagc
    68941 atggcgcgcg ccgcagatgt gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta
    69001 gggctggcac cgaccagcag caccaccgcc acgctggtta tgggcgatgc cctcgctgtc
    69061 gcgctgttaa aagcacgcgg ctttactgct gaagattttg cgctctcaca cccaggcggc
    69121 gcactgggtc gtaaacttct gctgcgcgta aacgatatta tgcatacggg cgatgagatc
    69181 ccgcatgtta agaaaacggc cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat
    69241 cttggtgtga ctgtcatttg cgatgacaat atgatgattg aaggcatctt taccgacggt
    69301 gatttacgcc gtgtcttcga tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg
    69361 atgacgccgg ggggaatacg tgtgcgccct ggcattctgg ccgttgaggc actgaactta
    69421 atgcagtccc gccatatcac ctccgtgatg gttgccgatg gcgaccattt actcggtgtg
    69481 ttacatatgc atgatttact gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa
    69541 tgagcaaagc aggtgcgtcg cttgcgacct gttacggccc tgtcagcgcc gacgttatag
    69601 caaaagcaga gaacattcgt ctgctgatcc tcgatgtcga tggcgtactg tcagatggcc
    69661 tgatttatat gggcaataat ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg
    69721 gcattcgttg tgcgctcacc tctgatattg aagtcgctat cattaccggg cgaaaggcta
    69781 aactggtaga agatcgttgt gccacattgg ggatcactca cttgtatcag gggcagtcaa
    69841 acaaactgat cgcctttagc gatttgctgg aaaaactggc gattgccccg gaaaatgtgg
    69901 cttatgtcgg cgatgatctc atcgactggc cggtaatgga aaaagtgggt ttaagcatcg
    69961 ccgtggccga tgcgcatcca ctgttaatcc cgcgcgccga ttacgtgacg cgcattgctg
    70021 gcggtcgtgg tgcagtgcgt gaagtttgcg acttattact cctggcgcag ggcaaactgg
    70081 atgaagccaa agggcaatcg atatgagtaa agccagacgt tgggttatca ttgtgctgtc
    70141 actggcggtt ctggtgatga tcggcattaa tatggccgaa aaagacgata ccgcccaggt
    70201 ggtcgtcaac aacaatgatc ccacctataa aagcgagcat acggacacgc tcgtctataa
    70261 cccagaaggg gcactaagct atcgattgat tgctcaacac gttgaatatt attccgatca
    70321 ggccgtttcg tggtttacgc agccggtact taccacgttt gataaggata aaatcccgac
    70381 atggtccgta aaagcagata aagccaagct gaccaatgac cggatgctct atttatatgg
    70441 acacgttgaa gtcaacgcac tcgtgccgga ctctcaactt cgcagaatca cgacggataa
    70501 cgcgcagatc aatctggtga cgcaggatgt tacctctgaa gacctcgtca cgttatacgg
    70561 aacaacattt aactccagcg gtctgaaaat gcgcggcaac ttacgcagca agaacgccga
    70621 gctgattgaa aaggttagaa catcctatga aattcaaaac aaacaaactc agccttaatc
    70681 ttgtgcttgc cagctcactt ctggccgcca gcattccggc atttgccgta accggagaca
    70741 ctgatcagcc gatccacatt gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg
    70801 ttacctttac cggcaatgtc atcgtcaccc agggcaccat caaaattaat gccgacaaag
    70861 tggtcgttac ccgtccgggc ggcgaacaag gtaaagaagt gattgacggc tacggtaaac
    70921 cggcaacgtt ctaccagatg caggacaacg gtaaacccgt tgaaggtcac gcttcacaga
    70981 tgcactacga actggcaaaa gattttgtcg ttctgacggg taatgcttat ctgcagcagg
    71041 tcgatagcaa cattaagggc gataagatca cttacctggt gaaagagcag aaaatgcagg
    71101 ctttcagcga caaaggcaag cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca
    71161 aaaacaataa aggccagacc ccggcacaga agaagggtaa ttaattcgtt atggcaacat
    71221 taactgcaaa gaaccttgca aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc
    71281 tgaccgtcaa ctccggggaa attgtcggtc tgctggggcc aaacggtgcc ggtaagacca
    71341 ccactttcta catggttgtt ggcattgtgc cgcgcgatgc gggcaacatc attattgatg
    71401 atgacgatat cagtctgctg cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc
    71461 cgcaggaagc ctccattttc cgtcgcctca gcgtttacga taacctgatg gcggtactgc
    71521 aaattcgtga cgacttgtct gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag
    71581 agtttcacat tgagcacctg cgtgacagca tggggcagtc actctccggg ggtgaacgtc
    71641 gccgtgtaga aattgcccgt gcactggctg cgaatccgaa atttattctg ctcgacgaac
    71701 cgtttgccgg ggttgacccg atctcggtta tcgacattaa acgcatcatt gagcacctgc
    71761 gcgacagcgg cctgggcgta ctgatcactg accacaacgt gcgtgaaaca ctggcggttt
    71821 gtgaacgcgc ttatatcgtc agtcaggggc atttgatcgc ccacggcacg cctacagaaa
    71881 tcttacaaga cgaacacgtt aagcgtgtat accttgggga agacttcaga ctctgatagg
    71941 gtagaagttt gcgatgtttt agcaggagag tacgattctg aacatgaagc aaggtttgca
    72001 actcaggctt agccaacaac tggcgatgac gccacagctc caacaggcaa ttcgtctgtt
    72061 gcagttgtcg acgctggaac ttcagcagga gctacagcag gcgctggaga gtaatccgct
    72121 gcttgagcaa atcgacactc atgaagaaat cgacacccgc gaaacgcaag acagtgaaac
    72181 actggacacc gccgacgcgc tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc
    72241 cagttgggac accatttaca ccgctggtac accatccggc accagcggtg actacattga
    72301 cgacgagctg ccggtctatc agggcgaaac gacgcagacc ttgcaggatt acctgatgtg
    72361 gcaggtcgag ctgacaccgt tttccgacac tgaccgcgct attgctacct ctatcgtcga
    72421 tgccgttgat gacaccggtt atctgactgt cccgctggaa gatattctcg aaagtatggg
    72481 cgatgaagag attgacatcg acgaagttga agccgtcctt aagcggatcc aacggtttga
    72541 tccggtcggt gtggcggcaa aagatctgcg tgactgtctg ctgatccaac tctcccaatt
    72601 cgataaaacc acgccatggc tggaagaggc cagactgatc attagcgatc atctcgatct
    72661 gttagccaat cacgacttcc gcactttaat gcgcgtcacg cggctgaaag aagatgtgct
    72721 gaaagaagcc gtcaatctga tccagtcgct cgatccgcgc cccgggcagt cgatccagac
    72781 gggcgaacct gagtatgtca ttccagatgt gctggtgcgt aagcataacg gtcactggac
    72841 ggtagaactc aacagtgaca gcattccgcg tctgcaaatc aaccagcact acgcctcgat
    72901 gtgcaataac gcgcgcaacg atggtgacag ccagtttatc cgcagcaatc tgcaggatgc
    72961 caaatggttg atcaagagcc tggaaagccg taacgatacg ctactgcgcg tgagtcgctg
    73021 tatcgttgaa cagcagcaag ccttctttga gcaaggcgaa gaatatatga aaccgatggt
    73081 actggccgat atcgcccagg ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac
    73141 gcaaaaatac ctgcatagtc cacgaggcat ttttgaactg aagtatttct tttccagtca
    73201 cgtcaatacc gagggcggcg gcgaagcttc ctccacggcg attcgtgcgc tggtgaagaa
    73261 attaatcgcg gcggaaaacc cagcgaaacc gttgagcgac agcaagttaa cctctttgct
    73321 gtcggaacaa ggtatcatgg tggcacgccg cactgttgcg aagtaccgag agtctttatc
    73381 cattccgccg tcaaaccagc gtaaacagct cgtttgaccc aaccgataag gaagacacta
    73441 tgcagctcaa cattaccgga aataacgtcg agatcaccga ggcactgcgc gaatttgtta
    73501 cagccaaatt tgccaaactt gagcaatatt ttgaccgaat caaccaggtc tatgttgttc
    73561 tgaaagtgga gaaagtcacc cacacctcag atgcaacact gcatgtaaac ggtggtgaaa
    73621 ttcatgccag cgcggaaggt caggacatgt acgctgccat tgatggctta attgataagc
    73681 tggcacgcca gttgaccaaa cataaagata aactgaaaca acactaattg tccgggcaat
    73741 tagcatgtgc atggcggcct gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc
    73801 gagcctgttc cactgtttga gtgggcaggt tcttaggtga aattatgaca aataatgata
    73861 caactctaca gcttagcagt gttcttaaca gggaatgtac gcgaagccgc gtccactgtc
    73921 agagcaaaaa acgcgccctg gaaatcatca gcgagctggc ggcgaaacaa cttagtctgc
    73981 cgcctcaggt ggtttttgaa gctatcctga cgcgggaaaa aatgggcagt accggtatcg
    74041 gcaatggtat tgccattccg catggcaaac tggaagaaga tactctgcgc gccgttggcg
    74101 ttttcgttca gctcgaaacg cctatagctt tcgatgccat tgacaaccaa ccggtagatt
    74161 tactatttgc gttgctggtt ccggctgacc aaaccaaaac gcacctgcat actctgtcgc
    74221 tggtggcgaa acgtctggcg gacaaaacca tctgccgccg tttacgcgca gcccagagcg
    74281 atgaagagct gtatcaaatc attacggata ccgaaggtac tccggatgaa gcgtagttat
    74341 tcggtaatgt ctcttttaga cgttgtgagg agaaacagta catggtactg atgatcgtca
    74401 gcggacgttc aggttcaggt aaatctgtcg ccctgcgtgc gctggaagat atgggttttt
    74461 actgcgtgga taaccttccc gtagtgttgt tacccgatct ggctcgaacc ctggccgatc
    74521 gcgagatttc tgccgccgtc agcattgatg ttcgtaatat gccggagtca ccagaaatat
    74581 tcgaacaggc gatgagtaac ctgcctgacg ctttctcacc gcaactcctg ttcctggatg
    74641 ccgaccgtaa taccttaatt cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca
    74701 gcaaaaacct gtcgctggaa agtgctatcg acaaagaaag cgatttgctg gagcctctgc
    74761 gttcgcgagc ggatctgatt gtcgatacct cagaaatgtc cgttcacgag ctggcagaaa
    74821 tgctgcgtac ccgtctgctg ggtaaacgtg aacgtgaact gaccatggtc tttgagtctt
    74881 tcggcttcaa acacggtatc cctatcgatg cagattacgt ctttgacgtg cgcttcttgc
    74941 cgaacccgca ctgggatccg aaactgcgtc caatgacagg tcttgataaa cctgtcgccg
    75001 cgttcctcga ccgccacaca gaagtacaca attttatcta ccagacgcga agctatcttg
    75061 agctatggtt acctatgctg gaaaccaaca accgtagcta cctgacggtc gccattggtt
    75121 gtaccggcgg gaagcaccgt tcggtgtata ttgcagagca actggcagac tacttccgct
    75181 cgcgcggtaa aaacgtccag tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt
    75241 caagcaaact gttgaaatca caaacaagct gggcatgcat gcccggcctg caatgaagct
    75301 gtttgaatta atgcaaggtt ttgacgcaga agtgctgtta cgtaatgacg aaggtaccga
    75361 ggccgaagcc aacagcgtta ttgcactgct gatgctggat tctgccaaag gacggcagat
    75421 tgaagttgaa gcgaccggtc cacaggaaga ggaagcactg gccgccgtta tcgccctctt
    75481 taattctggt tttgatgaag attaatcttc atcactttga cattcaaatc ccttcaagct
    75541 cccccgacaa taagaaaagc gcgtgctgaa atcgttctca atcaacgtca tttgtacatt
    75601 ttgtgcgctt ttcactttca gaagaacctt aagaaaacct taagaagcat tgtttaggtt
    75661 tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg agatggcatg
    75721 attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg ttatgcgcat
    75781 ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg tggcgataaa
    75841 gagatccgcc gtgagttaaa gtactacgcg catcttggtc gccggttaaa agactggagt
    75901 ggaataccgc gctatcacgg taccgttgag acggattgcg gaaccggtta tgtctacgat
    75961 gtgattgcag attttgacgg caaaccctcc attacgctga ccgaatttgc tgaacaatgc
    76021 cgttacgaag aagacattgc acaactgcgc cagctactga aacagctgaa gcgttatttg
    76081 caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg tcaccgcatc
    76141 agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagcac attgattcca
    76201 ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag agcggttatg gaaacgattt
    76261 atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg tgagagcaaa
    76321 acgctggccc tcacttcgcg cgaagcttaa tccagctggt gctgttgcat aaacgactca
    76381 ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctacgcac gtagcccgat
    76441 ggcgcggaga ctttgaaacg aagtggatta ggtaatacag cagccagtaa tgcagcttcc
    76501 gagcgggtaa gtttgctcgc gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca
    76561 aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg ctttttgctc
    76621 cagactgttt ctatccccag cgttaatcca gcttccagcc cttttcgtac ccagctacgc
    76681 ccatcccata aaaagagatt tttggccgtc tgctgagaaa tcgttgaagc accgcgaata
    76741 cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa tggaagcgac atcaaagccc
    76801 cagtgctcag gaaatttctg atcttctgcg gcaatcaccg ccagtcccat ccacggcgag
    76861 atttgatcca tactgaccca gtcagaatgt gccacgtaac gaaaattgcc atgcagccag
    76921 gcgctgacct gtcgctcgac cattaccgct gagaagggaa caggcgcaac actaaacaac
    76981 gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcaaaag aaaacggcga
    77041 acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc acgcgggaaa
    77101 ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata tatgctgggg
    77161 tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacat ggcacatgct
    77221 ccgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg gtcaaacgca
    77281 gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa ccaagcggtt
    77341 ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg gtgcattcgc
    77401 taccaagact ggcaaaattg cttaaattct tcgccgcgcc aaaacccccc ggcacaatca
    77461 acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg cgcgttattc
    77521 gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc ttcgcccgtt aaatggttga
    77581 taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa
    77641 tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc
    77701 tcagaattac gccaattttc ttcattgtga tcatcctttc gcaattgact gaaacacatt
    77761 aattttttta ataaaaatgg tacgcatcac acatttaact gattcatgta acaaatcatt
    77821 taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca
    77881 tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc
    77941 cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag cacttctacg
    78001 tcgtgacgcc actcttcttt catctcttca atccattcac cgacgttatc ttcccaggca
    78061 ggaaggtcag gagactgaat ttgctgaccc agctgttgca gatggcgtaa ccccactgac
    78121 cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc
    78181 agattcgact ccagcacgct gacatagccc ggcatcatct tctcaaacac cgctaacccg
    78241 tcggtgatca gcttcggtcc tacaagtgcg agatactgtt ccagcatggg aatatcgagc
    78301 aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc atcctgggta
    78361 tcccagaatt tcttgatcat cgcggttagc gccggaaccg aaagcggctt actcagcaca
    78421 tcatccattc cagcattgag gtactcttgt ttgtctttca gcacgttagc ggttaaggcc
    78481 accagcggcg gtaaatcctc gcgcggataa cgcttcgtca gttcacgaga gatatccagc
    78541 ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc cggtttaaac
    78601 atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa tttttccagc
    78661 acagaacgcg caacaatcac gttcagttca atgtcttcca ccagcagcac attcagcgcc
    78721 ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgctac cgacggtgcg
    78781 tggatcgtca acgtaaaggt tgaacctttg ccctgttcgc tggtaaccgt aatatcgccg
    78841 cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc ggtggcaggt
    78901 ttaccgccat gactgtcttt cacctggtaa tacatggcga aaattttatc cagctcatcc
    78961 tgcggaatgc cgataccaga gtcttccact tcaaaatgca gcatatcgcc ttcatcgtag
    79021 cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact gatgagattc
    79081 cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta
    79141 ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca atgcggaaag attttccaga
    79201 tcggcaagga agctggtgaa atcaaccggc tgattatcaa gctggacctt acgccgttcc
    79261 atcttatcca tgtcgataat atcgttgaag atattcccga gcgtgacggc cgaaacatgg
    79321 atggttttga gatatttttc ctgctcggcg gtgagttcgg tatccagcag aatgcggctc
    79381 aggccgacga taccattcag cggtgtacgc aattcgtgac tgatggtgga gataaacgtc
    79441 gttttgtcgc ggctggcccg ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg
    79501 cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg cactttacgg
    79561 atttcaaagc aggctttgcg tccgtcggga taatccagcc actgctcata ggtcagtgac
    79621 acattatgac ggaacacttt ttcatcggtt tcaatgactt tcgcagcggc ttccggtgag
    79681 taaacatcag caggtttcag atgaaccagt tgtttttcgc tttttccggt cagcagctcc
    79741 atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac caggtcgggt
    79801 gaagcgtcaa ggaaggaacg taagaatgag gattgctgct cgagctgaat ttgtgtctct
    79861 tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc tgcttccgct
    79921 ttttcacgaa cggcgatttc ctgatttagc tgggcaatat tatcttttaa ctgaacgttg
    79981 aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagtcgtga cagacgttgt
    80041 cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca cggcgtaatc
    80101 agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc atgcagcacc
    80161 atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc cagtaacatt
    80221 gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc cagcagacga
    80281 atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc aattctgaca
    80341 gttacgttat aaattgtgtg agaaatgcgg aattcccctc aatggcctac gccgttggag
    80401 gtagccacgg acgtcccagc gccgatccct gtacgccatg ttcattcaga taacgatcca
    80461 gctcgaccat tcccgtccat cgattttcgc accacagcgg agcaagcagc gtcgggcgac
    80521 gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga atcatttctc
    80581 cggcagtgag cgtgtaatcc tccagttcaa ttccgttcaa acgccccgct tcccaggctt
    80641 tcgccataat actgcctttc acaatatgca gcggatgcag ctttatgccg tccacgccgg
    80701 tttcaaccac tcgctccagc gtttgcaaac attccgtctg cccttcgcca ggcaggccga
    80761 caattaagtg ggaacacacc ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat
    80821 aacaggcaaa atcatggccg cggttgatgc gatgcagcgt tttgtcgtgg gcggtttgta
    80881 gccccagctc cagccacact tcgtaaccct ggtccttata ttcgcaaagc agatccagca
    80941 ccgcatccgg cacgcagtcc gggcgggtac caacacacaa accgacaata ttggcctggc
    81001 tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg
    81061 cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt
    81121 gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa cgaggccaca ttacagaatg
    81181 tgcagccacc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg
    81241 tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac atattgacta
    81301 atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa gcctgccatt
    81361 tttagcccaa ttcggcgatg acctggatca atcgtcccgg cccgctttta tcaactgcat
    81421 aatcaatcaa aattaccgaa atttcatgca caatcacata aatcactttt gcttatcttg
    81481 tgtcagattt ttttatctcc ttatggattt taggcaaaaa cagtagcatg aaacgttatt
    81541 accaattaag gcagtataaa atgctggttt tctcgtcagt tcaaggcagg ataagggtta
    81601 acacgccttt atgacagtca ggaattggct gtttctctaa cgacgcccct tttagcctta
    81661 aatagaaaat ccactttaat ttcagccaat taataaagaa ttttgcgcta aagcacattt
    81721 ctgtaccaat aagcttgcca tttgacctgt atcagctttc ccgataagtt ggaaatccgc
    81781 tggaagcttt ctggatgagc agcctgctca tcatatttat gcagtaattg agatcccctc
    81841 ttcaccgtat taaccgatgc gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg
    81901 acacgcaaac cccgtcgcca cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg
    81961 ttcccgcaga gcctggggga ggttcacgat atgttgtacg ataaatccct tgagagggat
    82021 aactgtggtt tcggcctgat cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt
    82081 actgcaatac acgcactggc ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa
    82141 accggtgacg gttgcggctt gctgttacaa aaaccggatc gcttttttcg catcgttgcg
    82201 caggagcgcg gctggcgttt agcaaaaaac tacgccgtcg ggatgctctt cctgaataaa
    82261 gatcctgaac tcgccgctgc cgcacgccgc atcgttgaag aagagctgca acgcgaaacg
    82321 ttgtcgattg tgggctggcg tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc
    82381 ctctcctctc tgccacgcat tgagcaaatt tttgtgaacg ccccggcagg ctggcgtccg
    82441 cgcgatatgg agcgccgtct gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc
    82501 gacaaagact tctacgtctg tagcctgtcg aatctggtca atatttataa aggtctgtgt
    82561 atgccggcgg atctgccgcg cttctacctg gacctcgcgg acctgcgtct ggaatcggct
    82621 atctgcctgt ttcaccagcg cttttccact aacaccgtac cgcgctggcc gctggcgcag
    82681 ccgttccgct acctcgcgca taacggtgaa atcaacacca tcaccggtaa ccgccaatgg
    82741 gcgcgtgcgc gtacctataa attccagacg ccgctgatcc ctgacctgca cgacgccgcg
    82801 ccgttcgtca acgaaaccgg ctctgactcc agttcgatgg ataacatgct ggaactcctg
    82861 ctggcaggcg ggatggatat catccgtgcc atgcgtctgt tagtgccacc agcctggcag
    82921 aacaacccgg atatggaccc ggaactgcgt gccttctttg actttaactc catgcatatg
    82981 gagccgtggg atggtccggc agggatcgtg atgtccgatg gccgtttcgc cgcctgtaac
    83041 ctcgaccgta acggtctgcg tccggcacgc tacgtcatca ccaaagataa actgatcacc
    83101 tgcgcctctg aagtcggtat ctgggattat cagcctgacg aagtggtcga aaaaggccgc
    83161 gtagggccag gcgaactgat ggtgatcgac actcgcagtg ggcgcattct acactcggca
    83221 gaaaccgatg atgacctgaa aagccgccat ccctataaag agtggatgga gaaaaacgtc
    83281 cgccgtctgg taccgtttga agatctgccc gatgaagaag tgggtagccg cgaactggac
    83341 gacgacacgc ttgccagcta ccagaaacaa tttaactaca gcgcggaaga gctggattcg
    83401 gtgatccgcg tactgggtga aaacggtcag gaagcggtcg gttcgatggg cgacgatacc
    83461 ccattcgccg tgctctccag ccagccgcgc attatttacg actacttccg ccagcagttt
    83521 gctcaggtaa ccaacccgcc aatcgacccg ctgcgcgaag cgcatgttat gtcgctcgcc
    83581 accagtatcg gtcgtgaaat gaacgtcttc tgtgaagcag aaggccaggc gcaccgttta
    83641 agctttaaat cgccgattct gctctattcc gatttcaaac agctcacgac gatgaaagag
    83701 gatcactacc gcgcagatac gctggatatc acctttgacg tcactaaaac cacgctcgaa
    83761 gcgacggtga aagagctgtg cgacaaagcc gaaaaaatgg tacgtagtgg caccgtgctg
    83821 ctggtgctct ccgaccggaa tatcgctaaa gatcgcctgc cggttccggc tccgatggcg
    83881 gttggcgcga tccagacccg tcttgtcgag caaagcctgc gttgcgatgc caacatcatc
    83941 gtagaaaccg ccagcgcccg tgatccgcac cacttcgccg tgttgctggg tttcggcgcg
    84001 acggctattt atccatacct tgcctatgaa acgctgggcc gcctggtaga cacccacgcg
    84061 attgccaaag attatcgtac cgtgatgctc aactatcgta acggcatcaa caaaggcttg
    84121 tacaaaatca tgtccaaaat gggcatctcc accatcgcct cttaccgctg ctcgaaactg
    84181 tttgaagcgg tcggtctgca cgatgatgtc gtgggcctgt gcttccaggg ggcggttagc
    84241 cgcattgggg gggctagctt tgaagacttc cagcaggatc tgctgaatct gtcgaaacga
    84301 gcctggctgg cgcgtaagcc tatcagccag ggcggcctgc tgaaatacgt gcacggtggc
    84361 gaataccacg cctacaatcc ggacgtggtg cgcacgctgc aacaggcggt acagagcggc
    84421 gagtatagcg actatcagga atacgcgaag ctggttaatg agcgtccggc aaccacgcta
    84481 cgcgatctgc tggcaattac gccgggtgaa aacgcggtca acattgctga tgttgaaccg
    84541 gcaagcgaac tgtttaaacg cttcgatacc gccgcgatgt ctatcggcgc gttaagcccg
    84601 gaagcccacg aggcgctggc ggaagcgatg aacagcatcg gcggtaactc aaactccggc
    84661 gaaggcggtg aagacccggc gcgctatggc actaacaaag tgtcgcgcat caagcaggtg
    84721 gcgtccggtc gcttcggcgt tactccggcg tacctggtca atgccgacgt cattcagata
    84781 aaagtcgccc agggcgcgaa gccgggtgaa ggaggtcagt taccgggtga taaagtcact
    84841 ccttacattg ccaaactgcg ctattcggtg ccgggcgtga cgctgatctc cccgccgccg
    84901 caccacgata tctactctat tgaggattta gcgcagctga ttttcgacct caagcaggtt
    84961 aacccgaaag cgatgatctc cgtgaagctg gtttccgaac cgggcgtagg caccatcgcg
    85021 actggcgtgg caaaagctta tgccgattta atcaccatcg caggctatga cggcggcacc
    85081 ggcgcaagtc cgctttcatc ggtgaaatac gcaggctgtc cgtgggagct ggggcttgtt
    85141 gaaacccaac aggcgctggt tgccaacggc ttgcgtcaca agatccgttt gcaggtcgat
    85201 ggcggcctga aaacgggtgt cgatatcatc aaagcagcga ttcttggcgc ggaaagtttt
    85261 ggcttcggca ctggcccgat ggtagcgctt ggctgtaaat atctgcgaat ttgccacctg
    85321 aacaactgcg ccacgggtgt agcaactcag gatgacaaac tgcgtaagaa tcactatcac
    85381 ggcctgccgt tcaaagtgac taattacttt gagtttatcg cccgtgaaac ccgcgagctg
    85441 atggcacagc tgggggtaac gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa
    85501 gagctggacg gtttcaccgc caaacagcag aaactggcac tgtcgaagct gctggagact
    85561 gctgagccgc atgctggtaa agcgctctac tgcaccgaaa acaacccgcc gtttgataac
    85621 ggcctgctga acgcgcagtt gctgcaacag gcaaaaccgt ttgtcgatga acgccagagc
    85681 aaaaccttct ggttcgatat ccgcaacacc gaccgttctg tcggcgcatc gctttcaggc
    85741 tatatcgccc agacgcacgg cgatcagggg ctggctgccg atcctatcaa agcgtacttc
    85801 aacggcaccg caggccagag cttcggcgtg tggaacgcgg gcggcgtgga actgtacctg
    85861 accggtgatg ccaacgacta tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt
    85921 cctccggttg gctcagcctt ccgcagccat gaagcgagca ttatcggcaa cacctgcctg
    85981 tatggcgcga ccggtggtcg tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg
    86041 cgtaactccg gtgctatcac cgtggtagaa ggcattggcg acaacggctg tgaatatatg
    86101 acgggtggta tcgtctgtat tctgggtaaa accggcgtta acttcggcgc aggcatgact
    86161 ggcggcttcg cttacgttct cgatgaaagc ggcgatttcc gcaaacgcgt taacccggaa
    86221 ctggtcgagg tcttaagcgt tgacgatctg gcgatccatg aagagcatct gcgcggtctt
    86281 atcaccgagc atgttcagca taccggttct cagcgcggtg aagagattct ggcgaactgg
    86341 tcaaccttcg ccactaaatt tgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg
    86401 ctgggtcacc gtagtcgtag cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca
    86461 acaatgagtc agaatgttta tcaatttatc gacctgcagc gcgttgatcc gcctaagaaa
    86521 ccgctgaaga tccgcaaaat tgagtttgtt gaaatttacg agccgttttc cgaaggccag
    86581 gccaaagcgc aggctgaccg ctgcctgtcg tgcggcaacc catactgcga gtggaaatgc
    86641 ccggtacaca actacatccc gaactggctg aagctcgcca acgaggggcg tatttttgaa
    86701 gcggcggaac tgtcgcacca gaccaacacc ctgccggaag tatgcggacg tgtctgcccg
    86761 caagaccgtc tgtgcgaagg ttcctgcacc ctgaacgatg agtttggcgc tgtgaccatc
    86821 ggcaacattg agcgttatat caacgataaa gcgttcgaaa tgggctggcg tccggatctg
    86881 tccggcgtga aacagaccgg taaaaaagtg gcgattatcg gcgctggccc ggcgggtctg
    86941 gcgtgtgcgg atgttctgac gcgcaacggc gtaaaagcgg tcgtcttcga ccgtcacccg
    87001 gaaattggcg gattgctgac cttcggtatt ccggctttca agctggaaaa agaggtaatg
    87061 acgcgccgcc gtgaaatctt caccggcatg ggtattgaat tcaaactcaa taccgaagtg
    87121 ggccgcgacg tgcagctgga cgatctgctg agtgattacg atgccgtgtt ccttggcgtc
    87181 gggacttatc agtcaatgcg cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca
    87241 gcgctgccgt tccttatcgc caacaccaaa cagttaatgg gctttggtga aacccgcgac
    87301 gaaccgttcg tcagcatgga aggcaaacgc gtggtggtcc ttggcggtgg cgacactgcg
    87361 atggactgcg tgcgcacgtc tgtacgccag ggggcaaagc acgttacctg tgcctatcgt
    87421 cgtgatgaag agaacatgcc gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc
    87481 gtagagttca aattcaacgt ccagccgctg ggtatagaag tgaatggcaa cggcaaagtc
    87541 agcggcgtaa aaatggtgcg cacagaaatg ggcgaaccgg atgccaaagg ccgtcgccgc
    87601 gcggagattg tggcaggttc cgaacatatc gttccggcag atgcggtgat catggcgttt
    87661 ggtttccgtc cacacaacat ggaatggctg gcaaaacaca gcgtcgagct ggattcgcag
    87721 gggcgcatta tcgccccgga agacaacgac aacgctttcc agaccagcaa cccgaaaatc
    87781 tttgctggcg gcgatatcgt ccgtggttcc gatctggtgg tgaccgctat tgccgaaggt
    87841 cgtaaggcgg cagacggtat tatgaactgg ctggaagttt aagcctgtcc cagcgccgcc
    87901 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    87961 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    88021 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    88081 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcacctg gtcgatagcg
    88141 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    88201 gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc
    88261 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    88321 acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt cgccagacag
    88381 tgtctggcga attaacacca ttgggaattc cgcagacacc atctgcagaa ttcccatcgg
    88441 caaagatttg gcagacgctg tctgccaaat ctttccccct gccctggtcc acctacgtcc
    88501 gtttactttc cgttaaaaac cctgatgccc gcagctttta tgaaaaagag acgctccgct
    88561 gtggttggtc tgtccgccag ctagagcggc aaattacgac ccaattttat gagcgaacac
    88621 tactgtcaca tgacaaatca gccatgctgc aacaacacgc tcctgccgag atgcatattc
    88681 ttccgcaaca ggcgatacgc gatccctttg tgctcgaatt tctggagttg aaagatgaat
    88741 actcagaatc tgattttgag gaggcgctga tcaaccacct gatggatttc atgctggaac
    88801 ttggggatga ttttgccttt gttggtcggc agcgaaggtt acgcattgat gacaactggt
    88861 ttcgggtcga tctgctgttt ttccaccgcc gtttacgctg cctgctaatc gtcgatctaa
    88921 aagtgggcaa attcagctat agcgatgccg gacagatgaa tatgtatctc aactacgcca
    88981 aagagcactg gacactaccg gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga
    89041 aaggagccgg cgaagcgcat tatgcactgg ctggtttgcc taacaccgtt ctggcgagtg
    89101 aatataagat gcaactacct gatgaaaaac gactcgcgga tgaactcgtt cgaacacagg
    89161 cggtgttaga ggaaggctat agacgccgtt aatttcagcg gtttcgaatt ctccagacac
    89221 tgtctggaga atctttaaaa cgaactaagt tttaagccat taaatcagcc ttaaccttca
    89281 ccacaacctt tttaatctca ccaggttcgc cgacaacgca tcctggttta tgcggttctc
    89341 ccggcataaa cacggcgaac attcccggtt ttaagatgat ggcttgctcg ttctcaatgg
    89401 cgctgcaaag ctggtaatca tcctcatggt ggaactcttc acactgacgc gcagtgcctg
    89461 ccgtgccaaa cagaatccgt tcctcaccgt ttaataacag ctggatatca atgtattgct
    89521 cgtgcagctc cgcttttttc tcaacgggcg attgagtgtt aaacgtcatg acattcataa
    89581 agatattgtc gcactgtaat tcgtaacgac ccggtgcttt ttcttgcggc ctggcggcta
    89641 aggctagtgt taacgcgtcc tgtaacgcag gatgcaatcc ggcagacggt aacgactgta
    89701 cttcacccat tatcataatt tttctccctg ggccaacaac gcagccccaa gtaaacctgc
    89761 atcgtggcgg tagtgcgccg ccagtaaatc aacatgaaat gccgctggct cctgcgccag
    89821 atacgtttcc accaacgcca gatacccttc tgccagacca acgctgccac cgaccactac
    89881 gcactggcaa tcagttgtgg ctttaatatc agcgatcagc cttgccagca catgtgcgga
    89941 gcagtgaatc agttgctgcg cctgctcatc gccctgcccg gcgcgtgtga aaatagttct
    90001 cgcatccgcg cccgccagtt caccctgcgc tgccgctgca ataccgcgac cagaagcaat
    90061 cgcttccaca caaccagtgc gtccacagcc gcagactgga ccatgaggat cagccagcgt
    90121 atgcccgatg tgccccgcca gaccgccagg gccggtgagc agtttgccgc cgctcactac
    90181 accgccgcca acaccagtgg aaacggtgat aaacaccatc tcggttatat cgccttccag
    90241 cgcctgatac tccgcccacg ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatc
    90301 ggtaagttgt tccaaagttt tgactaacgg aaagtgtagc aatccaccga gattatgcgg
    90361 attcagcgcc agcaggctgc cgtcgcgaat aattccggtt gaagcgatag caacccgctg
    90421 cgcatgaact tgcaacggag agactaatgc ggataaggca tcatgcaagg cttctggtgt
    90481 ctggctggct ggcgtaggaa gttcacgacg atcgcggatc tgcccatcag cgccaatcag
    90541 cgcggcggca agtttagtac cgccgatatc aatcgccagt gtggtcatag caccgccttt
    90601 ttcatcgctg tgttgtacca ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc
    90661 gtcaccgccc acgcgccgtg gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc
    90721 ccttcggcaa tcacccgaca tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc
    90781 tcttctggcg tttcaggcgt ggtatagcca gaaagcgtag tgccaataat ttcggctccc
    90841 agcttttggc atgccaggcc gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg
    90901 tgatggtgaa tacgtgccag cagcgtttca acaggcaccg gacgcggtcg gtcggtgccg
    90961 tcaatggcga taatgtccgc gcccgcctgc gccagcgcat caacatcttc aatataggcc
    91021 gtgatgcgta ccggagaatc ctccagatcg cgtttcacaa tcccgataat tggcacgctc
    91081 accaccgcac gcgtggcttg cagatttgcc acaccttcaa tgcgaatggc aaccgcgccc
    91141 gcctgttctg ccgctaatgc catggcggcg acgatttcgg gtttatcgag cgggctgtcc
    91201 ggaaccggct ggcaggagac aatcaggcca ccgttagcag cgattttttg atccagttgt
    91261 gcaagtaacg acatacatct tcccttagcg aaaggcccgg tacagagacc gggcaacagg
    91321 attaactttt ggttttgact aaatcgtttt tggcgcggcc aaatggcacg gcaccgctaa
    91381 atggtttgcc gtcgatagcg tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa
    91441 cgcgggaagg catatccaga ccaatcagca ggatcaccac aaatgtcaga ctgaacgaga
    91501 gcgatgccag cgcagtaccc agatccagac gttgagcgat caacgcgccg atgattgggg
    91561 ccagtgcacc gcccaatgcg ccgacgttgt aggtaaagcc caggcccgct gcacgctggt
    91621 cggtatcgaa ataaccgcca atcagtttcg gtaagatccc ggcgatccct tgtccaagca
    91681 tttgctggaa gaacagtaac agaccgagca cccagacgtt tgcgccgcca atcgcaaata
    91741 ccggaataat cagcagctgc gaggccagca gactacaaac gtacgctttg cgggttccca
    91801 gccagtcacc gaggaagcca ccgacgcagc accccaccgc cgcgccaaag ccactaaaga
    91861 acagcacatt ggcgacagta tgcgggtcat aagccagatc ggttttcaga tacgttggca
    91921 gcagcgcctg aatcggccat gagtagagga aagcaaacaa cacgaccacc atcagcatta
    91981 cgcccgttgg ccagcgtttg cccgtactct gcaccataaa gctgataaag attgcggcgc
    92041 ataacagccc gagtacagcg acgattgcgg cattttgcag gttaccggcg aagcagaacc
    92101 acagcgcagt agccgccgcc agcgtcatta cgatattggc aatgcgatgt tcaccacggt
    92161 aaagaatgtc taccatcgtg cgtactggtg ctttaccccc gtgtttctct ttccagtctt
    92221 ccgcttccgg gatgttttta cgcagccaga gagcaaagat gattggcaaa atgccgataa
    92281 agaacagcgc tctccagccc cagaccggaa ccaccagact atagacctga gcggcaacga
    92341 cggcccccac agagaagcct gaaatcaaaa aaccactggc tttgttacgc agatgttttg
    92401 gccagctttc aatgacatag gtggcgctgg aaccgtattc acccgccatc cccatgccga
    92461 tgaccagacg cgcgataaac atggtgatgt agcctggcgc aaagccgcag gccagcgtcc
    92521 cggccgagaa gagaacgatg ctggtgacca ttgccagacg acgcccgtag cggtcaccca
    92581 tagcgccgag catcaggccg ccgaaccagc gagagataaa ggctgcggag atcagacttg
    92641 ccgcctgcac cgtcgtcagc ccgaattcac cctgtacttc ggtgagtacc agggcgatta
    92701 aaacgaaatc aaaaccgtca agcagatatc ccaaccaggc agcggaaaat gcgcgccatt
    92761 gtgcacggtt gagatggcga taccacggga tattctgggt tgtagtactc attgtgagtc
    92821 tcccgcggtg ggcgatgccc acacgctttg gtatgaaaat tgtagggtac agatgcgttt
    92881 atttcccctc acccggtagg ggcgagcgag gggaaaacaa ctcacccgcg ctcttgcatc
    92941 aactgctggg ccagcgcctt cagttctggc agatattttt catctaccgg tccaaacggt
    93001 ttgcggcaca gcggcacaga aacgacatcc atataatgca ggacagtttt caggccgcgg
    93061 aatacgcccg ttttgatcag taaatcaatg actttgttgc actcagtttg cagtttctgc
    93121 gcggtctgga tatcgccttc tttcagcgcc ttcacgatcc cctgatatcg ccagcccatg
    93181 atgttgtaag tactgccgat accaccatca gcgcctgcca gcaagccgga agcgaagatt
    93241 tcgtcgtaac cgttatagag caccagatca ggatgttcac gacggatctg ttccatctga
    93301 tagagatcgc cagaagtctg tttcagcgca ccgacgccag gcaacgtaac cagggtgttg
    93361 atctgatcca gggtcagttt taccccactc agggctggaa tgttgtacac caccatcggc
    93421 aaaccatccg ccgaatcaat aattgcccga tagtgatcgc agtgttcttc aaagctgaaa
    93481 ggatagtaga acggcgtgac ggcggagacg gcatcgaagc cataacgttt agccgatgcc
    93541 gcaagttgtt ggctttcggc ggtgctgacg caaccgacgt gggcgatgag tttaatctta
    93601 cctttcgcct cttcagcgac gatttccagt acctgttcac gctcggaaag gctttgtaca
    93661 aaggcttcgc cggtagaacc acccacgtat aaaccgtcga tgccctgctg aatattgaac
    93721 tgaaccaggc gacgcagact cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg
    93781 agtgcggcca ttacgccacg taaattcgtt gccataaata cctctgaagt gatgcttatc
    93841 tgataaacga tataccttta tacctgttat accagatcaa ttaagcaaca ccccatacag
    93901 aaagcttata atgcgatctg cttcactaaa gtggcattat ttctttttgt tggtggtctg
    93961 accgaaggcg tgccaggtag cagagacgct gttgagatgc gattgcaacg cacgatcggc
    94021 ttcatcggga tcgtggcggc ggatcgcgtc aacgatcgca atatgctgtt gatagctaac
    94081 gttgttatgc tcgtgcagag cctgatcagc cacggttgga cgtgcggcga tcagccagtc
    94141 gagcaaggca acgtggatcg ccatgaagat tgggttaccg gggatctccg ccagcacgcg
    94201 gtggaaatca acgtctgaac gaatgaatgc cgcgttgtta tccagcgact gactgttgat
    94261 ttccagtgct tttgccagca aatcgatttg ctcatcggtg gcgtgttcag ccgcatagcg
    94321 caccaggctg gattcaaaga acagacgtaa ttgttcgaaa tgggcaatcc cgccaggatg
    94381 agaaaggaaa tctttcgcca tgccggaaag ctcgccgatg atggtgtccg cagaaggacg
    94441 tgagacgcga gcgcgttcgc cgttgtttat ctgcaccaga cctttgcgtt ttaacgctgc
    94501 cagcgcttcg cgcaccgaag gacgcccgac gttaaagaac gccatcagtt cgcgttcaga
    94561 cggtaattgt tcaccttcgc caaattcacg acggcggatc atctgttcca gctcttcttc
    94621 caccatttcg gagagttttt tacgcgccag cgggcggctg cgtaagttgc ggccaattgc
    94681 aggtgaagaa tcttcggttt gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca
    94741 gtgtgattaa catcatcagt gacatcctat cacaggattg aaagtagggg gaaatggcgg
    94801 ggttttctct ttgtgcctca tcattactat aattaactga ataattaact attgcgaaaa
    94861 attaatgtaa cgcagataaa aacatcccga ttgaattatt cataagacta ttaacaagta
    94921 ttatgaatat tatgaatgtg ttcttacaaa ataatcataa gcgcatattt tttaatgaaa
    94981 aatcacctca tctacaatta aaaacacgac atccgcacca taaatagcct tgcaaaaaat
    95041 ataacatcat tgttttcaat ctgccgttta tgggattgac cattttcttt tgacacggag
    95101 ttcaacaatg ttcggcataa ttatatctgt catcgtatta attacgatgg gctatttgat
    95161 cctgaaaaac tacaaacctc aggtggtgct ggctgccgca ggtatcttcc tgatgatgtg
    95221 cggtgtctgg ttagggttcg gtggtgtgct cgatcccgcc aaaagcagcg gctacttgat
    95281 cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac cgcattgctg gattggggct
    95341 gtcgattatg gcagtgggcg gttatgcccg ctatatggag cgcatagggg caagtcgcgc
    95401 gatggtgagc ttgttaagcc gcccgttaaa actcattcgc tcgccgtata ttattctgtc
    95461 ggcaacttac gtcatcggcc aaatcatggc gcagtttatt accagcgcct ccggtctggg
    95521 tatgttgctg atggtcacct tatttccgac gctggtgagt ctgggagtaa gtcgtctctc
    95581 tgcggtggcg gttatcgcaa ccaccatgtc cattgagtgg gggattctgg aaacgaactc
    95641 catttttgct gcccaggtcg caggaatgaa aattgccact tacttcttcc actaccagct
    95701 tccggtcgcc tcttgcatca ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc
    95761 tttcgacaaa aaagataaaa atatcaatca cgaacaggca gagcaaaaag ctctcgataa
    95821 tgtcccgccg ctctattacg ccattttacc ggtgatgccg ttaatcctga tgctcggctc
    95881 gctgttcctt gcccacgtcg ggctgatgca gtcagaactg catctggtgg tggtgatgtt
    95941 actgagtttg acggtgacgc tgtttgttga gttcttccgc aagcataact tgcgcgaaac
    96001 aatggacgat gtgcaggcgt tttttgacgg catgggtacg cagtttgcca acgtggtaac
    96061 gctggtggtc gcgggtgaaa tatttgcgaa aggcttaacg acgattggca ctgtcgatgc
    96121 ggttatcagg ggggcggagc attctggtct gggcggtatt ggcgtgatga ttattatggc
    96181 gctggtcatt gccattggtg ccattgtgat gggctcaggc aatgcgccgt ttatgtcatt
    96241 tgccagtctt attccgaata tcgcagccgg actacatgta ccagcggttg taatgattat
    96301 gccgatgcat tttgccacga cgctagcgcg cgctgtttcg ccgattactg cggtggtggt
    96361 cgttacgtca ggaattgcag gcgtttcgcc ttttgcggta gtgaagcgga cagcgatccc
    96421 catggcagtc ggtttcgtgg tgaatatgat tgccactatc acgctatttt attaagcgat
    96481 taaaaagaca aaacaggccg ccagggcctg ttttcatttt acttcacaac gcgtaatgcc
    96541 ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag gatgagtgtc atcatcgtga
    96601 tctggcttgt cgccatcaat aaccgacata acggtttcgt tgtctgccga tgcctcttca
    96661 tcattcatga tgctggcatc ttcatcgtag gcagcttcag gttcaaacat cgtgcctgcg
    96721 ccattttcac gggcgtagat agccagcacg gcagccagcg gcacagaaac ctgacgcgga
    96781 atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg ccagttccag attgccgaca
    96841 gctcgcggcg caatgttgag tacgatttgc ccgtcacgcg catattccat aggaacctgc
    96901 acgccaggga gcgtcacatc caccaccagg tgcggcgtga gctggttatc cagcaaccac
    96961 tcatagaatg cacgcagcag atagggacga cgtggtgtta gctgtgacaa atccatacag
    97021 attaactccg gcccagacgc atttcacgtt cagcttcagt taaagaagca aggaaagagt
    97081 cacgctcaaa gacgcgggtc atatagcctt tcagctcttt cgcacccggg ccgctgaact
    97141 cgatgcccag ttgcggcaga cgccacagca gaggagcaag gtagcaatcg accaggctga
    97201 actcatcgct caggaagtac ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt
    97261 cgcgcagttg cttacgtgcg gcatcggctt cagaagctga accgttgatg atggtgttca
    97321 tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag acggctttca ccgcgagcta
    97381 ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc atccagatat tccataatga
    97441 tgcgagattc ccacagggtc agctcacgat ccaccagggt cggaacgctc tgattcgggt
    97501 tgaggtcaat cagatcctga ggcggattgt ccttttccac gtgttcgatc tcgaaactta
    97561 cacctttctc agccagcaca atgcggacct gatggctata gatgtcagta ggaccggaaa
    97621 acagcgtcat taccgaacgt ttgttggcag cgacagccat gaaaacctcc aggtatagtc
    97681 agaattttta ctgctaccag ccaccaggtg gccagtcaga agttgtgtta cccagtaagg
    97741 aacgactctc tttgttcgaa aatcaaacaa aaaatgagca atacccgaca tttgggcaga
    97801 aaattggatg atagtttacc agattttgcg acctttgtgg tgagtcgatg ccggaaatgg
    97861 ggaaaaagag atgcgcttta gtctgaaata gttgacttag tcccttattg gcgatgtggt
    97921 ttttgtttta cctgtctgtc aggtggcagc aaaaagcaac ttcccatttt ttacgctgat
    97981 gcagatttta gccataaaaa aacccgccga agcgggtttt ttcgcaaatt gttttctgcc
    98041 ggagcagaag ccaattaacg tttggagaac tgcggacgac gacgtgcttt acgcagaccg
    98101 actttcttac gttcaacctg acgagcgtca cgagtaacga agccagcttt acgcagttca
    98161 gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga taccgtgacg gatcgcacca
    98221 gcctgaccag agataccacc acctttaacg gtgatgtaca ggtccagttt ctcaaccatg
    98281 tcgaccagtt ccagcggctg acgaactacc atgcgagcag tttcacgacc aaagtactgt
    98341 tccagagaac gttggttgat tacgatttta ccgttgcccg gtttgatgaa aacgcgagct
    98401 gcggaacttt tgcggcgacc agtgccgtag tattgatttt cagccattgc ctataatccc
    98461 gattagatga ttagatgtca agaacttgcg gttgctgtgc cgcgtggttg tgctcgttac
    98521 ccgcgtaaac tttcagttta cggaacatag cacgacccag cgggcctttt ggcaacatgc
    98581 ctttaaccgc gatttcaatc acacgctcag gacggcgagc aatcatctct tcaaaggtcg
    98641 cttgtttgat accaccgatg tggccggtgt ggtgatagta cactttgtca gtacgcttgt
    98701 tgccggttac agcaactttg tcagcgttca gaacgatgat gtaatcaccg gtatctacgt
    98761 gcggagtgta ttccgctttg tgcttaccgc gcaggcgacg agccagttca gtagccagac
    98821 ggcccagagt tttaccggtc gcgtcaacaa cataccagtc gcgttttacg gtttctggtt
    98881 tagctgtaaa agttttcatt aaaagcttac ccaataaaaa gttacacgtt ggtgaacacc
    98941 caaacgtctt caattgttga ggttcacacg acaaagtccg gcaaacctac cccttcgaat
    99001 agcctatgcc agcacacaaa aagttttggg aaaaaaactt tcttgtaacg tggggtcgca
    99061 ggattataga gaagtcgggg tcaaagatcg accccttttt gtgatttgtg gcaggtttta
    99121 acccgccaaa tgctcgcgct tcagatactc ttcgctttgc atctcttgca gacgtgacag
    99181 gcaacgctgg aactcaaatt tcagccgctc gccctgataa atgtcatata gcggcacttc
    99241 tgcactcacc actaatttga catggcgctc gtaaaactca tccaccagcg caataaaacg
    99301 tcgcgcttcg ctctccatca accgcgtcat aactggtaca tcaaacaaca tgactgtgtg
    99361 aaagaggcgt gagagcgcaa tatagtcatg ctgactgcgg gcgtcgacgc acagcgtagt
    99421 aaaagagacc gccagcgtct ggttctcgac gcccattgtc gccagcggcc gatggttgat
    99481 ttctaatgtc ggtgaatttt ctcgtttcgc ccccgccagc gccaaccata gtttatccat
    99541 ttgcgcccgg gtttcatcgt tgaggggcga aagccacaga tgcgcctgag tgagtgtacg
    99601 cagtcgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc
    99661 gattgcaggc aaaaaacgcg cacgttgtag gccatttcga taaagttcgt ccggcggaat
    99721 atttgacgtc gctaccaggg taataccgcg ggcgaacagg gctttcatca gaccgccaag
    99781 tagcatggca tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc
    99841 tgctttaaag cgatcggcaa taatttccag cggatcggtc tgcccctgca aagcagttag
    99901 ctcttcatgt acccgcagca taaaacggtg aaagtgcaga cgctgtttcc gctctcccgg
    99961 caggctttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat
   100021 atataagcca cgcactggcg tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac
   100081 ccgcgccatt agcccactcg tcctgggggc tggaggcgtg ctattgatga gttcctgata
   100141 aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc
   100201 attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattgttatt
   100261 ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac
   100321 gcgatatgca gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc
   100381 gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat
   100441 gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat
   100501 gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa
   100561 taaagctgaa ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga
   100621 attactggat accatggcgc acgactatcg ccagctgtat caacacatgg cgaaaagctc
   100681 cagcagcctg ctgccggaac tgtctgctga agcaaacccg ttccgtaatc gtctggcaga
   100741 gtctgaagcc agcaacgatc aggcaccggt gcaaatgcct cgcgactatt ctgaaggcgc
   100801 atccggccta ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt
   100861 gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg
   100921 tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag
   100981 agtaggaata atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt
   101041 aactctctcg gcgtcatttc aggccgttgc gtcgattcca ggccaggttg ccgatcaggc
   101101 ccctctcccc agtctggccc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg
   101161 ggtggaagga acggccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg
   101221 tgatgattta ccggatcaac cggcacaacc cttcgaaggt ttaggctccg gtgtcatcat
   101281 caacgccagt aaaggctatg tgctgactaa caaccatgtg attaatcagg cacagaaaat
   101341 cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca
   101401 gagcgatatc gccctgttgc aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc
   101461 cgactccgat aaattgcgcg tcggtgattt tgccgtggcg gtcggtaacc catttggcct
   101521 tgggcaaacc gccacctctg gcattgtttc cgcattaggc cgcagtgggt tgaatcttga
   101581 aggtctggaa aactttatcc agacagatgc ttccattaac cgcggtaact ccggcggtgc
   101641 actgttaaat cttaacggtg agttaattgg catcaacacg gcaatccttg cgcctggtgg
   101701 cgggagcgtc gggattggat ttgccatccc cagtaatatg gcgcgaacac tggcgcagca
   101761 gcttatcgac tttggtgaaa tcaaacgcgg tctgttaggc atcaaaggca cagagatgag
   101821 tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt
   101881 gttgccaggt tctggctcgg caaaagcggg cgtcaaagcg ggcgatatta ttaccagcct
   101941 caacggcaaa ccgctgaata gctttgctga gttgcgctct cgtatcgcaa ccaccgagcc
   102001 gggcacgaaa gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac
   102061 gctcgatacc agcacctctt cgtcggccag cgctgaaatg atcacgccag cgcttgaagg
   102121 tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa ggtattaaga tcgatgaggt
   102181 tgtcaaagga agcccagctg ctcaggcagg cttgcaaaaa gacgatgtga tcattggcgt
   102241 caaccgcgat cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc
   102301 catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc tatctgctga tgcgttaatg
   102361 tcgtaaaccg ggcatcaggc tgacgtgtga tgtccggtta actcgtggta tgctgctgcc
   102421 gttccctttt ttaatgacgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt
   102481 ggattaattg tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg
   102541 ctttccactc cgcaatttga cagtaccgat gagacgcctg ccagctataa tctggcggtt
   102601 cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg gtttgaacac caactctcac
   102661 aaccagcttg agatccgcac cctgggatcc ggtgtaatca tggatcaacg cggttatatc
   102721 atcaccaata aacacgtcat caacgacgcc gatcagatca tcgtcgcctt acaggatgga
   102781 cgtgtttttg aagcgttact ggttggctct gactctctaa ccgatctggc ggtgctgaaa
   102841 attaacgcca caggcggttt accaacgatt ccgatcaatc cacgccgcgt accgcacatt
   102901 ggcgatgtgg ttctggcgat cggtaacccg tacaacctcg ggcagaccat tacccagggg
   102961 attatcagtg ccacgggtcg aatcggtctg aacccgaccg ggcggcaaaa cttcctgcaa
   103021 accgatgctt ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggcgaa
   103081 ctgatgggca ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt
   103141 atcggctttg cgattccttt ccagttagcg accaaaatta tggataaact gatccgtgat
   103201 ggccgtgtga tccgtggcta cattggcatt ggtggacgtg agatcgcccc attacatgcg
   103261 cagagtggcg gcatggatca actgcaaggg atcgtggtta acgaagtgtc gcctgatggc
   103321 ccagcggcaa atgcaggcat ccaggtcaac gatctgatta tctctgtgaa taacaaaccc
   103381 gctatttccg ccctggagac aatggatcag gtcgcggaaa ttcgccctgg ttcggtgatc
   103441 ccggttgtcg tgatgcgtga cgataaacag ttaacattgc aggtcaccat tcaggaatat
   103501 ccggcgacca actaaggtca gcgtaaaaaa acaaaaaccg gagtctgtgc tccggttttt
   103561 tattatccgc taatcaatta cttattaacg aactcttcgc ccagggcgat atctttcttc
   103621 agcgtatcca gcataccttc cagcgcgttc tgttcaaatg cgctcagggt accgatagat
   103681 ttacgctctt ccacgccgtt tttacccagc agcagcggtt gagagaagaa acgtgcgtac
   103741 tgaccgtcgc cttcaacgta ggcacattcg acaacgcctt gttcgccctg cagtgcgcga
   103801 accagagaca gaccaaaacg tgcagctgcc tggcccatag acagggttgc agacccgcca
   103861 ccggctttcg cttcaaccac ctcagtaccc gcgttctgga tacgtttggt cagatcagcc
   103921 acttcctgct cggtaaaact aacgccagga acctgtgaca gcagcggcag aatggtaaca
   103981 ccagagtgac cgccaataac cggcacttca acttcgcctg gctgtttgcc tttcagttcc
   104041 gcaacaaagg tgttggaacg aatgatatcc agcgtggtaa cgccgaacag tttgtttttg
   104101 tcataaacac cggctttttt cagcacttca gcagcaatcg caactgtggt gttaaccggg
   104161 ttagtgataa taccaatgca cgctttcggg caggttttcg caacttgctg taccaggttt
   104221 ttcacgatgc cggcgttaac gttaaacagg tcggaacgat ccatacccgg tttacgcgct
   104281 acacctgcag agataagaac gacatctgcg ccttccagcg ccggagtcgc atcttcacca
   104341 gaaaaacctt tgattttcac agcagtaggg atatggctca gatcgacagc cacaccggga
   104401 gtcactggag cgatatcata cagagagagt tctgaacctg aaggcagttg ggtttttaac
   104461 agtagtgcaa gcgcctggcc aataccgcca gcagcgccga ggactgcgac tttcatccta
   104521 aactccttat tatattgata aactaagata tgttgctccg ctgccgcgac cttaatccac
   104581 aaaattgccg tttacaatga ccacatctca agaatgtgta gtcacgcaag tttagcgttt
   104641 atgcatttaa ttgccgtaat caggaaccta acttacgtaa ttaacagtcg cttatgcatt
   104701 agcgcaacat tccaacaggt ggtgacaata taccctaccg ttcagccaaa acaacatcaa
   104761 tttgataaca attaatttac ttttaagcag aatttgcatg ccgtgacgca ggcatgtttc
   104821 tcaataacga aatttgataa aatcccgctc tttcataaca ttatttcagc cttcttcagg
   104881 gctgactgtt tgcataaaaa ttcatctgta tgcacaataa tgttgtatca accaccatat
   104941 cgggtgactt atgcgaagct cggctaagca agaagaacta gttaaagcat ttaaagcatt
   105001 acttaaagaa gagaaattta gctcccaggg cgaaatcgtc gccgcattgc aggagcaagg
   105061 ctttgacaat attaatcagt ctaaagtctc gcggatgttg accaagtttg gtgctgtacg
   105121 tacacgcaat gccaaaatgg aaatggttta ctgcctgcca gctgaactgg gtgtaccaac
   105181 cacctccagt ccattgaaga atctggtgct ggatatcgac tacaacgatg cagttgtcgt
   105241 gattcatacc agccctggcg cggcgcagtt aattgctcgc ctgctggact cactgggcaa
   105301 agcagaaggt attctgggca ccatcgctgg cgatgacacc atctttacta cccctgctaa
   105361 cggtttcacc gtcaaagacc tgtacgaagc gattttagag ctgttcgacc aggagcttta
   105421 atctctgccc cgtcgcttct ggcggcgggg aaaatgttgc ttttccctct caccccccct
   105481 gctttctcct gcgattaatt taacgaatag tgcgttttat cgcgacatat cattcacata
   105541 atgaatacat gggttaaaaa aagcacatta tgcaaaattc attatctaat tgaaaagaat
   105601 agaattaata ataaataatc gtgtttttaa ttcttttttg ttattaaaac tcacattttt
   105661 aacacttagt atcaactgaa acagttagcg tggtattaat tagttcaata attagtgtat
   105721 acttgatttt gtgatatggg tcacgaaaca aaggcccagc taaaagatta tgtcgaggta
   105781 aaaatcatga aaatcaaaac cactgttact gcattaagcg tactttctgt tctctctttc
   105841 ggtgcattcg ctgccgactc cattgatgct gcacaagcac aaaatcgtga agcaatcggg
   105901 accgtatccg taagtggtgt ggcgtcttcg ccaatggata tgcgtgaaat gctgaacaaa
   105961 aaagcggaag agaaaggcgc aacggcctac cagattactg aagctcgtag cggtgacacc
   106021 tggcacgcta cggctgaact gtacaaataa accctcatcg tcttgtccga cgatattgcc
   106081 cccggttcgg gggctttttt tgcgctaatg acgaacatta aaacgcaaat gcccttccag
   106141 ctcttcctct gcctcatcaa acagcaatat taacgcgcca aaacggcgac gcgttttctc
   106201 tcccagatga acaaattcaa tctcaagtgg tagcggcagg acatcgttca ttaacacatc
   106261 ccatagtgag tcgagatcgc gtaccttatc tttcgccaga ccaaaggctt ggctgaagtc
   106321 gcgataaaaa tcctcctgac tctcaatctc atcaaaatca aaggtataaa tattcatctg
   106381 ttgccaccgt cacgtttcac ggcgagttct gccgttcatc gctaagtata gccatgaaaa
   106441 aaccgacgct tcgggcgtcg gttttgactt aactatcggt caacgcatgt tgatacttat
   106501 ggagcatccc cgccagccga tgaaccggtt ccgtcacctg cggtggcgct tgccagatgc
   106561 gtagcttttc ctggtagatt tcaagttctt ccagcaactg gccaaagtac cgacgacgtt
   106621 tatcatcgct acgggcagat atcacatggt ctgctgtgcg gcgcatttgt cggtgaaacg
   106681 ccgataaatc ctcgttaacc gggatcggcg catcacgcag gcgctggtgc gcgataatca
   106741 tcgtcagcgc caggcgaaat ttcggcaaat cccctgggaa cttattcatc agcaaaaaca
   106801 gctgctgata aagtgccggg aggtgattct ctttacgacg tgccacattg gtagtcatcg
   106861 cggaaacagc ggcagaaaca aactgattaa gcagtacacg tccagtcctg tcgcgcgatt
   106921 tatcccgcac cagcaaaata acggtgaacg cgagcacaca gccgacgatt tgccctaatg
   106981 cgctgtcgag aaactgactg aaatggaaag tcatcgggtt atccagcacg ataatattta
   107041 tggtgctggc cagcgccccc atcgagccca gtcgccgttt ctgtacttct ataccgagga
   107101 aaaatcccag cactgccagg ctaatacaca gcaataacat gctttgttgg gtattaggga
   107161 taatcaccaa aaagtagagc agccctaacg gcagcgccgc cagcgtccca tagataaagt
   107221 cgatcgccac catgcgtgga ttcggcaaac gcattgccag tgacgtcacg accgcaatca
   107281 tcaccattgc accactgccg gaagtccagc ccgtccacag ccagaaaagc gtccccagaa
   107341 tgcaggaaag tgtggttcgc cagaagttaa ccatcgcatg atgacgttcg gctgactcta
   107401 ctttgacttc cggttcgcct tgcaggatct cttcttcagt agcgttgatt tttgtgttac
   107461 tgataacgcc gcgcttgaga agctgataac gcgttgccgc cgcgacccag ctataaatgg
   107521 tgacaggcgt ttcccgttcc ccggtccagg cgataactct ccgcaggcgt ttgagctgct
   107581 tgtggacgtc ctgcgcggtt tctaccggcg tgtcaaaaaa ttcgcggaaa gtatcagtga
   107641 tcaattccgg gcgcgtattc tgaataagat aagtttcgca ggattgggta atcagcgtca
   107701 gcgatagcgt attgatcgct tttaaacgtc gattggcccg cgcccagcgg gaagattcca
   107761 tattcaggtt gctgcgcata ccttgtaacg ccgtggtgcg tcgcaccaga tcgccccagg
   107821 ctttatcgac aacttcacca tcgccatgtt tgatacagag ttgcattaat tgatattgcg
   107881 cgaccagcaa actttccagc tctcgatcca cttcttgttt gatcgatcgc ggagaaaaga
   107941 gcaaatccgc cataatcgca cacacaatac cgataacgat ctcgctacaa cgttcgacgg
   108001 caaactgcgg cgtaagcaat ggttccggct gaatggtgat cacaatgatc agcgcggtat
   108061 aaccggccag cccccacgca tacgagtttt ctattcgtac cagcgaggat atccaggtac
   108121 aaaaaccggc ccagatacag cacaccagaa tcatcaataa tggtgcgcgg atcatcgcaa
   108181 tgatgattac cagtccagca atacagccaa taaatgtgcc gatgatgcgc aaaaagccac
   108241 gatagcgaat agcgccagaa tacggttcac ctcccgcagc aaaggccgga ccggcggcaa
   108301 caatcgccgc tgtcagtacc gcccagcgtg gcgtttccag ctggaagtga aagccaacaa
   108361 acagcgccag tacaatggcg gtcgccagtt ttaccgcaaa gcgaatatgt tgattagcaa
   108421 tggagaaaat acccatcgtg attaaccaaa ctcacgcagg cgatgggcca ttttacggaa
   108481 gaacgaatcc tggctttcgt cgcgatcttg tttgccagtg accaccactg tagcagtggt
   108541 gcccgcaggc cagatgttct cttgctggtt gtcgagacga atacgtaccg gaacacgttg
   108601 cgccaaacgt acccattcaa ggttagagtc gatggtcgcc atccctttgt cgtcacgcgt
   108661 gctgctggcg ttggtgaccc ctgcggcaac actatcaaca gtccctttca gcactttgtt
   108721 actgccaagc ggcgtgatct ccgcacgata cccagggcgc accccttcca gcttagtttc
   108781 ttccatatag gccagtacat agaaggagtt ctgtttcacc agcgcaaccg ccgttgatcc
   108841 tcgagtaata aactcaccgg tatagacgtt gaggttggtc acccagccat ctgctggcgc
   108901 gcggatgact gtacgttcaa gatccagttt tgccagatca cgggtcgcct gcgctttcgc
   108961 taactgatgc agaaccgttt gtagtacgtt gttggcctga tcgatctctt cgcgagacat
   109021 cgcctgcaca ccgagacggt tacgacgtcc tgcctcctgg cgtttctcct gtgccagtac
   109081 ctgataataa gcaacatcgg cttgcgcttc ctcaagcgcc ttttgatagc gcggctggtc
   109141 gatggtgaac agtacctgtc cttttttcac cagctggtta tcatgaacat tcacctgggt
   109201 aatgagtcca gaaacgtccg gcgcgatcgc aacgacgtca gcgctaaagc gcgcgtcacg
   109261 cgtccacggg gattcggtgt aatagaccca ggcattaaaa attgcgatga aggccagaat
   109321 gactaatacg accgtgatgg ccgtacggga gaattttctt attagtgttt tcacttcaac
   109381 ctcaaacgaa cagtcgcgat atcaaataaa acaagcagca atagagcgcg gtgttgaaca
   109441 acgccggatg ccagacaaag tcgtagatac ctgttggcac aagtacccgg cgcaccagcc
   109501 agaaaatcgc cagtgataaa agcaattcaa aaaatatcgg tgggaaggac agcccaaaca
   109561 ccacgataac gggaaacaga ctcatgttga ccttggttgt atagagagag caggcgttat
   109621 tattttcagc atctgtcgcc gcagagaagg gcatggaaag ccgggcgaga gcaacattgc
   109681 tgtagattga tatttaatat attagcgtaa ctgttatgct gttatctata ttatgtgatc
   109741 taaatcactt ttaagtcaga gtgaataatg gaacgactaa aacgcatgtc ggtgtttgcc
   109801 aaagtagttg aatttggctc ttttaccgcc gccgccagac agctacagat gagcgtttcg
   109861 tccatcagtc agacggtgtc aaaactggaa gatgagttac aggtaaagct gttaaaccgt
   109921 agtacacgca gcattggcct gactgaagcc ggcagaattt actaccaggg ctgccgtcgg
   109981 atgctccatg aagtgcagga tgttcatgag caactgtatg ccttcaataa cacccccatc
   110041 gggacgctgc gcattggctg ttcttcaact atggcacaaa atgttctcgc cgggctgaca
   110101 gcaaaaatgc tgaaagaata cccaggtttg agcgtcaatc tggttaccgg aattcctgcc
   110161 cccgacctga ttgccgatgg tctggatgtg gtgatccgcg tcggcgcgtt gcaggattcc
   110221 agcctgtttt cccgccgtct gggcgcgatg ccgatggtgg tgtgcgccgc gaaaagctat
   110281 ctcacacaat acggcatacc ggaaaaaccc gccgatttga gtagtcattc atggcttgaa
   110341 tacagcgtgc ggcccgacaa tgaatttgaa ctgatcgcac cggaagggat ctcgactcgc
   110401 ctgatcccac aaggaagatt tgtgactaat gatccgatga cgctggtgcg ctggctgacg
   110461 gcgggtgccg ggatcgccta cgtgccgctg atgtgggtga tcaacgagat caatcgtggg
   110521 gaactggaga tcctgctgcc gcgttatcag tcagatccac gcccggttta tgcgttatat
   110581 accgaaaaag ataagctgcc gctgaaggta caggtcgtga tcaactcgct gacggattat
   110641 tttgttgagg tcggtaaatt gtttcaggag atgcacgggc gcgggaaaga gaagtaattc
   110701 atattgtact gttacgttgt acaaacctgt gccaacgggt tcccctcacc ctaaccccag
   110761 aggagcgagg ggaccgttcg tgcacgtaga aagattaatt atccttctga aaatactgaa
   110821 attattacgc agtaccgcca acagtcaggt tatcgacttt caacgttggc tggcccacgc
   110881 caaccggcaa actttgccct tctttaccgc agacacccac gccgttatcc agtttcaggt
   110941 cgttacccac catcgaaatc tgctgcatgg tttcgatacc ggaaccaatc aacgttgcgc
   111001 ctttcaccgg cttcgttact ttaccgtttt caatcagata tgcttctgaa gtggagaaaa
   111061 cgaatttgcc ggaggtgata tccacctgac cgccaccaaa gttcggtgca tagataccgt
   111121 actcaacgga ttcaataatt tcctgcgggg tcgatttacc cggcagcata taggtgttgg
   111181 tcatacgcgg catgggcaga tgggcgtagg attcacggcg accgttgcca gtcggcgtca
   111241 tccccatcaa acgcgcgttg agtttatcct gcatgtagcc tttcaggatg ccattctcaa
   111301 tcagcacgtt gtactggcct ggcgtacctt cgtcatcaat cgccaccgaa ccacggcgat
   111361 cgaccatcgt accgtcatca accacggtac acagttctga agccaccagc tccccaacct
   111421 gtccactaaa tactgaagtg ccacggcggt tgaagtcgcc ttccagaccg tgacctaccg
   111481 cttcatgcaa cagcacgccc ggccaacctg cgccaagtac taccggcatg gtgcccgctg
   111541 gtgcagcaac ggcagaaaga ttgaccagcg ccatacgcac tgcttctttt gcccatgcat
   111601 ccgcacggac ttcgccgtcg agatcggcaa ggaagaactc ataaccaaaa cgaccgccgc
   111661 cgccactggc accgcgttcg cgtttgccat cttcttcgac cagaacgctc acggaaagac
   111721 gcaccagcgg acggacatcc gccgctagcg tgccgtcggt ggccgcaacc agaattaatt
   111781 catagacacc actgaggctg gcagtcactt cctgtacgcg cttgtccgct tcgcgggcaa
   111841 ccttatcgac gcgacgcagg atatccagct tctcttcacg gctcatgctt tgcagcggat
   111901 ctaccgaggt atacaacggg ctatgctcta ccgcgcccag cgtctgtact ttaccatcac
   111961 cactatcacg gacgatggtg cgcgccgctt gtgcactctg ttccagcgcc agcaggctga
   112021 tttggtcagc ataagcaaat ccggtttttt caccactgat tgcacgcacg ccaacgccct
   112081 gatcgatgtt gtaagaacca tctttaataa tacggtcttc taaaacccag gattcgtgat
   112141 agctcgactg aaaatagaga tcgccataat caaggcgacg ttcggccagt tgaccgagga
   112201 tcgcgaacaa gtcctgatgt ttcaggccgt tcgccgctag caattgttca cttaccaggt
   112261 tcagactcat cgtttttgct actcgttagt tactgcagta gaggattttt tacggctgcc
   112321 ggatgcggcg tgaacgccct gtccggccta cggttatgtt ctggctcgtt ggcctttggc
   112381 aacgattatc ctatgagatt ggggcaatta cgcgccctcg tcaaatcatt gcgctttttc
   112441 tttacgcggt tgtcgcaaca cttcgttgat ttgcggatcg tccagcggac ccgaaatgtg
   112501 atagcgcaaa atggagactt tgctccacag cggtcccagc actttactgg cggcaaacac
   112561 tgccgcgcca acaatggggt taaccgcaaa ggccgcagcc acacccaccg tcgcagaaat
   112621 ctctggtgcg acaaccgctt ccatattcag gtcgcgacgt accagattta ccgacccttt
   112681 catagcgatg tccgcttcca ggccatccac cagcgtgtcg tcggtgtgca taacgccgtc
   112741 tttaatccac gcggtgctgc gaatggagtc aaaatagaac ccttcgccaa aagtgtctct
   112801 gaaatcaaaa cgcagcttac gcatcagggc atctacgctc aataagcgca gcaattgccc
   112861 ggcatgtccg gtattgattt cggtaatttc gcctttaccc agttgagtat gaatgatgcc
   112921 attcaacgtc gcctcatctg gttgccaggg tgctttgcgc cagtgtaaat cgtaatccac
   112981 attaaatgac gactggcgta tgggcgtcgt gacaccaaaa aattctgcgg cggcatcaat
   113041 tttctgccca cgcagttttc ctttcagcga ggtgcgttca ttccccggat tgttaaccca
   113101 ttcaccatcg gcagtgagcc gcgaaaaacc agtatcaatc agtccattag tcagcgttaa
   113161 tgtattgcca gaaatggtga tatcactgtc aatgcgcccg aatttttgcc cccagaacca
   113221 gcactctgcg catcgtattt gtgcgtccgg ccagccacgg aagctaatgc gctccgttgt
   113281 cgggaaaggt gatgaattcg ttgagtcacc atgagttttc gccacgcttg ggttgtaata
   113341 aagatatttg atattcgcca gccacggcgc gttattacgc atcgccagcg tggcgttgat
   113401 ttcacgccct tgcgcctcaa ccagggtgcc atttgccgtc ggttgcgaaa caatgctcag
   113461 gttattccat tgctgatttc ccagcgacaa cataggcgta cgtaacgtta tgtgttgtgg
   113521 gaaactcgct gcaccaccga cactctccgc agcgcctttc tggaacaggg ccagccactc
   113581 ggcaccattc atcggcggca tattgagttc aacgccactt tgttccggca acggcgggag
   113641 cgttttactg tctgccgccc aaatagcacg gtcgagcgtc agcttttgac cgagcaacca
   113701 gcggctattg aaatggttat ctgcaccagc ctgtccggtt aactcaaagc tgttgagatt
   113761 gccatcaacc ttaacgttta ccggcagtgg ttcacccgca ggtttggcta acggtgaagg
   113821 taagtgactg ctcacattct tcaggtcgcc gttcagctct acgttatagg tcgcgccagc
   113881 atgataaggc agatcaatgc ccactttacc atcccacgcc acgctgccac ttaatgcttc
   113941 gttcaccgct tcaggcagaa cgccggtttt cgctggttgc cagttaccgt tgagattcac
   114001 cgcgacctgg taggcttttg ccccttcttt ggtggaaaaa tccacgttca acggctgatt
   114061 aaaccagctt gctgtcagtg gttcactttg cagatcgcca ttgataaagc tgaatttacc
   114121 gctcaaattt ttcagggtgc tgtcgagtgg tttgataaac agactgttat tacgcagcgt
   114181 cacatcacct ttcgcggtta ccagttcgcc gttcagcggg atatcaagat gtaagcgagc
   114241 attcacatcg ccgtcgagct ggagttcttg cagggtcgca cccagagaat ctttcagcgg
   114301 tgtctcatca aagtaagggc caacggcttt acccggacct ttaatgtcag cgtcaatcag
   114361 cagtttttct tttgagtagt cagggatcac tgcggtgaga ttacttgcgc gcacgccgcc
   114421 cagattaacg ccatcggttt tcatccataa accgtcgtta ataaagtcca gttcaatatc
   114481 aaggttagtt aatgcaggcc agtccggctg gaaggcaaac ttcgcgttgc gcagcggtac
   114541 cagcacttca aactgacctt cgttgtgttt ataggggaag agttgcggat tgccaccata
   114601 aaccagcgtc gcgttatccg cttcaccgcc ctgaatcgcg ccacttaagt aatcaaccag
   114661 gtctttaccc atcaagtttt ccgggaaata gcgccaggct tgtgaaccat catcggtact
   114721 gatgccagcc agaataccca gccagggttc atcgttagct ggttgcaggt aacgaaaacc
   114781 gccgcgcgca tggacggctt tggctttaac gtcaatatta cgcccatcca gctggaaacc
   114841 tttgtcattg ttcagccagc ttatcgttgc ctggccgtcg gcgatttcca gtggcgcacg
   114901 gaataccgtt tcgtaaggca tctttgcctg cttcatcgac gcagtaagca aaccattttc
   114961 tacgctgccg gaaagcgtcc cggagaagtg ttccgcaccc ggtaataatt tccattgctt
   115021 ccaggccaga tcgctccacg atgcctgaaa acgggtcttg tctgccgcct gaagcgggat
   115081 atccagcgcc agcgtgttaa tcttgccgct cggttgtgtg gagcgccaaa catcacccag
   115141 tgcaggtgaa agtttcgcgg ccagcgggcg tatgccctcc aggcctgcca gctccagatt
   115201 actggcgcga atccggagtt cgtcactgcg tttattgtct ttgccgccaa cgtcctgttc
   115261 cggtatccag gccagcgtca atgctccgct cggccagggt ttgccgtcca tcgtgatccg
   115321 tgtatcggga atagagaact gccagcctgg attttcacgc gtaatatgcg cggtcagatt
   115381 atccaccgac agcgtatgcg tttgcttctc gcccaaccag ctggcaccgc cctgtttcag
   115441 ccagacgtca ccgccggtta catcgccttt gtcgatcgtc atccagcctt caagggagaa
   115501 ctgtgccgtt tccagcgcaa tattgtcctg catccattta ccgagccacg gcttcaggtc
   115561 gatgtcatcc gcctggagcc agacgcgacc attgcttaac aacccctcat catcgcgcaa
   115621 atccatgcgc acctgcatca cgccgtgctg tccggtaagg ctggagaggc ttacctggcc
   115681 ttctgcacgg tgtcgacgtg gatcgttcag ccaggtgagc tgtgggatcg ccagctcggc
   115741 gcgctgaccg gatggcgtca ggaaactgac ttcactgtcg cggagatcga aatggtcgaa
   115801 ctgacgaaga aacagatcgc tgatgtgact ggcttccaga ctatcattac caccgccgct
   115861 ggtgatagga gtgttggtgc gaaagcgcag ctgccagaaa gtgaggtcgc gaaactgcca
   115921 gcgcatatgt aacaggctct gccagacatc cagcgccaga gtaacgcgtt taaccgaaaa
   115981 ttcgccgcca tcttttagtt ctgcacggat gtcgtgcgct tcaagcgtcg gaccaaaatt
   116041 ctgccagctg gctgagagct gactggcttc taccggcatg ccagtcgcgg attctatttt
   116101 gttgaggatt tccggacgcc aggcgtcaag atgcggtaaa gcaatacgca ggccgctaac
   116161 cagcagggca gcgatcacaa cgagcgcggc tccagtaagc agtaaaatcc ccggcaatcg
   116221 cctcacccgt cactccttgt ctgctaaaaa tgtgactcaa aaaccctttg ccggatggcg
   116281 gcccagcatc tgtttacatc attacgacgt caaactgctc ctggttatag agcggttcaa
   116341 tttgtacttt aacctgttta ccaacgaaaa tttccacttc cgccagcgag tgtgactctt
   116401 cgcctttcaa ggcttcagct actgccggag aagcatagac caggaaacgg tcggaatcat
   116461 aagcatggtg tacacgaaca atctcgcgca tgatttcata gcataccgtt tccacggttt
   116521 tcaccgttcc gcgaccgtgg caggttgggc attcgttaca tagtacgtgc tcaatgcttt
   116581 cgcgggtgcg tttacgcgtc atctccacca gccccagcgc cgaaaaacca ttaacgctgg
   116641 ttttcacccg gtctttgctc aacgcctgct ccagcgagtg cagcacgcgg cggcggtgat
   116701 cttcattatt catatcgatg aaatcaataa tgataatccc gcccagatta cgcaaccgta
   116761 actggcgagc gatagcctgc gtcgcttcaa tattggtatt gaaaatggtg tcgtccagat
   116821 tgcgatgacc gacaaacgcc ccggtattga tgtctacggt ggtcatcgct tcggtctggt
   116881 cgataatcag atagccaccg gatttcagtt ctactttgcg ttccagcgct cgctgaattt
   116941 cgttttcgac atcaaagaga tcgaaaatcg gctggcgtcc ggtgtaatgc tccagcttgc
   117001 tggtcatctc gggaatgtac tccgaggtaa actccagcaa tgcttcgtaa gtcaggcgtg
   117061 agtcaacgcg aatgcggtcg agttcggcgt cggcgaaatc acgcagaaca cgctgcgcca
   117121 gcgccagttc gccgtacagc tgataacggg tctgtgggcg ctttttacgc tccataactt
   117181 tggtccagac gcgtttcaga taagcggcat cggaggccag ttcagcctcg ccaacccctt
   117241 cagctgcggt acggatgata aacccgccct gctcgtcgca atactctgcg accacttttt
   117301 tcaggcgttc acgttctgat tcgctttcaa ttcgttggga aaccccaacg tgagaagccc
   117361 ctggcataaa taccagatag cgagaaggca gcgtgatatc ggtggtcagg cgcgcaccct
   117421 tagtgccaag tggatctttc accacctgca ccatcagatc ttgcccctga cgaaccagtt
   117481 ccgagatgtc gcgcaccgtg aattgctttt gttcttcacc cgccacacat tcggtgtgcg
   117541 gcatgatgtc agatgcatga agaaacgcgg ctttatccag cccaatatct acaaaagccg
   117601 cctgcatacc cggaagtaca cgacttacac gacccttgta gatattgcct actattccgc
   117661 gtcgcgcctc acgttcaata tgaatttcct gcagaatacc gccatcaata tacgccactc
   117721 gcgtttccga aggcgttacg tttactaaca attcagccgt catgtttatc ccttttctca
   117781 cgcagtgcgt taaaattact taataattca tacgtttcaa ccagcggtaa gccgactacg
   117841 gcgtgatagc tgccatttat cttcctgaca aaacagccac ccagcccctg aataccgtat
   117901 gcacctgctt tatctaacgg ttcaccgctg gcgacatagc ccgcgatgtc ttcgtctgtt
   117961 aacgttctga aagtcacatc ggtgaccacc aggcaatcga gaatgtgctg gctgtcggcc
   118021 aacgccactg ccgtcattac ctgatgggtc tgacccgata atttgcgcaa catctgcgcc
   118081 gcatgctctg cgtcgcgcgg tttctccagt acttctccgt tcaggataac gatagtatcc
   118141 gcacccagca ccgggagatc ctgcgccgtt tgcgcgacac ccgcctgtgc tttctcgcgc
   118201 gccagacgca caacatactg ctgcgcgctc tcctgcggct gacgctgctc ctcaatgccc
   118261 gtaacaatac gttcaaaggt tacgccaagt tgcgcaagta actcctgacg acgcggagaa
   118321 ccggaagcta aatacagaga agtcatagaa acctttattg cactgcaaac tgctgacgga
   118381 ctttacgcat cagcaagaaa atccacggcc agagcacccc attgactacg ctactccaga
   118441 acacttccgg tctgaaagag acgttaatca ctaaaaactc tgcccagaaa acaataatat
   118501 ccaccaccag cgaaagcaac atgacgacca gcgcctgctg ccataatgcg aggttgcgga
   118561 aaagctggta tttcagcgcc accaggtaag caatgatgct catcgccaat acgcgtacgc
   118621 caagcgtcga gccgctgatc agatccagta tggcacccat cacaaaacct gtgcccacat
   118681 ttacgcgatg aggcaaggcc aggatccaat acaacaagat gagtaacacc cagtttggcc
   118741 ggaaaacaat caggttatcc ggccagggca tgatttgcag caacagcgca atgaggaaag
   118801 agagccagat tacccagcgt ccctggctac gatagctcgc cactattgcc ctcccggcgc
   118861 acgcgcaggc ggttgagcac cactttgcgg cggcgtagcc ctttgtggag agcgattagc
   118921 agcgggctgt gtgggcgcag caggcgcagt aactgcattt cctgtcgccg gttgctgcgg
   118981 agtcggctga gtgatccccg ttgccggttc aggtaacttt ggccccatcg cgtctggcga
   119041 aggcaatacc tgcggcatca tctgcatcaa acgttcatta gcaacacgat gcacctcttc
   119101 cggcgtcatc gggttagcgc cgttacgatc cgcgccccac agcagcagca gataacgcaa
   119161 acgttgcagt cctgcagtcg gacgcgcctg aatcacagta taagcgcgct gggtatcgag
   119221 ttttacggaa gagacaaccg cgaccggata gccttccggg aaacgaccgc ccagaccgga
   119281 agtcaccagc acatcaccaa cacgaatgtc cgtattcgct ggcagatgct caagctgcaa
   119341 atcatccgta caaccgttac cggctgcaat tacgcggata tcgttgcgca gcacctggat
   119401 tggcagcgcg tgggtcgcat cacaaatcag cagcacgcgg ctggtcagtt tagcgacggc
   119461 caccacctga ccaacaacac ctttgtcgct gataaccggc tggccttcat aaacgccatt
   119521 aacgctacct ttatcgataa caacttgatc gctataagga tcgttaaccg tggagataac
   119581 ctgagtcacc attttctgct catcctgacg cagcggggaa cccagtagct cgcgcagacg
   119641 cgcgttctcc tgtttgtatt gtccaagcat cagcagttca ctgtttttca gcaacagttc
   119701 ctgacgtaac gcccggtttt caagttctag ttggtcacgc gaggccagcg tctgcgatac
   119761 gccatccagc aattcacgag gagcattgga aacaaagtag aaaggactga cggcggtatc
   119821 catataagta cggatttgac tgaacgtccc caggcggctg tcggcaataa taatgccgag
   119881 cgccaccagc accgccagaa taaggcgaat ctgtagcgac gggccacggc taaaaattgg
   119941 cttcataagt tatgcgtatt ctcgtatcag accaggcagg gtaaacagac acttcccctg
   120001 cctgcatccg attactcttc gctgaacagg tcgccgccgt gcatgtcgat catttccagc
   120061 gctttgccgc caccgcgcgc cacacaggtc agcgggtctt cagcaacaac gactggaatg
   120121 ccggtttctt ccattaacaa acggtcaagg ttacgcagca gtgcgccacc accggtaagc
   120181 accatgccac gctcggagat gtcggaagcc agttccggcg ggcactgttc cagtgcaacc
   120241 attaccgcgc tcacaatacc ggtcagcggt tcctgcagtg cttcgaggat ttcgttggag
   120301 ttcagggtaa agccgcgtgg aacaccttct gcaaggttac ggccacgaac ttcgatttca
   120361 cgtacttcat cgcccgggta agccgaaccg atttcgtgct tgatacgttc tgcggtggct
   120421 tcaccgatca gagaaccgta attacgacgt acatagttga tgatagcttc gtcgaaacgg
   120481 tcaccaccaa tgcgcacgga agaggagtaa accacgccgt tcaaggagat aacagcaact
   120541 tcagtagtac caccaccgat atcaaccacc atagaaccgg tcgcttcaga aaccggcagg
   120601 ccagcaccaa ttgcggcagc catcggttct tcaatcagga agacttcacg ggcaccagcg
   120661 ccctgcgcgg attcacgaat tgcgcggcgt tcaacctggg tcgcgccaac cggcacacaa
   120721 accagaacgc gcgggcttgg acgcataaag ctgttgctgt gcacttgttt gatgaagtgc
   120781 tggagcattt tttcagtcac gaagaagtcg gcgataacgc cgtctttcat tgggcgaata
   120841 gcagcaatat tgcccggcgt acggcccagc atctgcttcg cgtcatgacc tactgcagct
   120901 acgcttttcg gtgaaccggc acgatcctga cgaatggcca ccacggaagg ctcattcaat
   120961 acgatgcctt gtccttttac ataaatgagg gtattcgcag tacccaggtc aatggacaag
   121021 tcattggaaa acatgccacg aaattttttc aacatactaa gggataatcc tgaaagctgg
   121081 ggcggaaaag aaaatccgct tactttacca accacacgca gcagcgacaa ggcgcaaaaa
   121141 tcatctgcta cggtgaaaat tagtgcagtt cgtttccttt gttacaaatc tctgcctgag
   121201 tccagaaagg cttaatgcat cagcagcatt cctcgcctgt ttgcaaccgc gtaaggtcat
   121261 tcatctgcat atgtgctgca acaatctggc gagcagacaa gcacactccc atgagacgca
   121321 gcgcgcgtta ttctacgtga aaacggatta aacggcaggt taaaccgagt atctttgtga
   121381 atattttttc acgttagtat caagtggctg tgaggacgcg aaaaaatccc cttgcccacc
   121441 tgtaacaccg cgctgaatca gggtctgcca ctcgcttcgc gaacgcacgc cggtggcgta
   121501 aacctgggtg ctggtcccgg agcaggcttc caccaggctt tgaaccagca gctgattctc
   121561 cgttcgcttc tcaatgtttc tgaccagccc cggatggagc ttgagtaact caacattaag
   121621 ttctttgatc caactggtgc ttaccagcgt caaaccagcc tggttgacgg ctacccgtac
   121681 ccctaaagca ttcactaaac gaataacagg ttgtaagcga ctgatatgtt gaccaacatc
   121741 ggcctctgca agttcaataa ttatgcgtct tcgttgcgat ttttcacatt gcattaacgt
   121801 atcgcgcagc caacgctgaa aacgcgggcg aatcagcgac tcaacggtaa cctgaatcgc
   121861 cagattttcc tctggccagt aacgcaatag tggaataaga cggctgatta gcaggcggtc
   121921 atactcttcc gataaaccaa actgcaagac catcggcata tactctgccg agctaacctc
   121981 ttcattacca tcgaagatgc ggcacatgag ttcacgatga tgaacccggc cttcgcgagt
   122041 aaccgccggt ttttgataaa ggcgcgggcc gccgcgactg agcatttgct cgataagcgt
   122101 acgccagcga acattaccgc gtcctttttc aggcaacgag tcatcgtaaa tagcccagct
   122161 attgccgccc tgcaatcccg cattacgcgt ggcagactct gcatgttcca ttacctgctc
   122221 ggtatcctga ccactacgcc aggcacagat accaatgtgg atcatatcgt cgcgatcgag
   122281 cattttattg ttcggcaagg tatcgacggc tttgattaac tgactggcga tgctctctgc
   122341 ctcttttaac gtccggtgcg gtaacagcgc agcaaaatca ctgcggtggt aacgcgccag
   122401 cagtgcgcca gggtagcgca tcataaatgt cgatagcaga ttcgtcagag agaagaactg
   122461 ttcttcaacc tggctgtgcc cccaggtatc actcaacata ttgaaatccg gcagacgaat
   122521 catcatcacg atcccgtggg tacctacttt ctcttgatct tccagtaacg ttgctaactg
   122581 attatcgaaa aagagtcggt tattgaggcc ggttttcatg tcctgggcgg cataagagcg
   122641 gatcagcgta tcaagacggc tgtgttgttc gcgtgcgttc tgaatttcac aaagcagcgt
   122701 atccagcgca ctgctggttc tgggcggcca ttcatagatg gttcccaaca cattagagcc
   122761 acgctcaccg tttaagatac gagtagcccg ggtttccagc aattcttgcc cggcaagttg
   122821 ccgttgtaac cagcgtaccg ccaggaagag cataagaatg ataaagccaa tcgcccccgt
   122881 gagcggcgcg gtagtcatta aagaatggaa atagttgccc atcggatcct gataaaccag
   122941 acgcagcgac atccccggat gctttatcaa cggaacgctc agttcacgaa acagatcgtt
   123001 ggtgccaacc ggacgataac taccatttct ggccagggta taaacctgct tatcaccatg
   123061 gagcaggtct acacgaacga tatctgccga catcattaat tcggtaattt gtggccttaa
   123121 tgtgctgaag tcattcgaca ctaggtgggt atcaatcgcc gtcgccaccg cctgaacacg
   123181 atgactaaac ttatactgaa tggcgttgta gaaacttagc gaacagccca gcaaagtcac
   123241 aaaaattgtt aacccggtga gcagcgtgac aaaggccgaa aatttcgtcg ttaatctcat
   123301 ccttgtgtta actccgatag tgaggaagcg ggcatactag caaatcagat ttatctcgca
   123361 atttattgcg cttcatcggc tttgcttttc cattagcgag tatagtcttc agaaattatt
   123421 ttccaatcca tcatgcacat gaggaccact tatgcaggcg ttacttttag aacagcagga
   123481 cggcaaaact ctcgcatcag tacagactct ggacgaaagt cgcctgccgg aaggcgatgt
   123541 cacggttgat gttcactggt cgagcctgaa ctacaaagat gcgctggcga ttaccggtaa
   123601 gggaaaaatc atccgaaatt ttccgatgat tcctgggatt gattttgccg gaactgtacg
   123661 caccagcgaa gatccgcgtt ttcatgccgg tcaggaggtg ttactcactg gctggggcgt
   123721 tggtgaaaac cactggggtg ggctggcgga acaggcgcga gtgaaaggtg actggctggt
   123781 tgccatgccg caagggctgg acgcgcgtaa agcaatgatt atcggtactg ccggttttac
   123841 cgccatgctg tgtgtgatgg cgctggaaga tgccggtgtt cgcccgcagg atggggaggt
   123901 tgtcgtgacg ggtgccagtg gtggcgtcgg cagtaccgcc gtcacgctgc tgcataagtt
   123961 gggttatcag gtcgttgccg tttccggtcg cgaaagtacc catgaatatc tgaaaagttt
   124021 aggtgccagc cgtattcttc ctcgcgatga gtttgctgaa tcccgtcctc tggaaaaaca
   124081 agtctgggct ggggcaattg acaccgttgg cgacaaagtg ctggcaaaag tgctggcgca
   124141 aatgaattac ggcggctgcg tggcggcctg tggtctggcg ggtggtttta ctctgccaac
   124201 cacggtcatg ccatttattc tgcgtaatgt ccgtttgcaa ggggtggatt cagtaatgac
   124261 gccaccagca cgccgcgcac aagcctggca gcgactggtc gccgatttac cggaatcatt
   124321 ctatacccaa gcggcaaaag agatatctct ggcagaggca ccgaagtttg ccgaggccat
   124381 cattaataac cagatccagg gtcgcacgct ggtgaaggtt aactaaccat ttagcaggga
   124441 ataataagag agggaactca ttttgaaatc attattcaat cggttaacgg gaaaagcggt
   124501 tagccggaca gctttcgtcg aacatcttgg tcatgaagtt gtacaacatc atccaaactg
   124561 gaaagtcatg atttcgactg accataaatt gatgcgcatt gatgctccac caaacagcca
   124621 ttattgatac gcctccgtcg cctgttaggt ttatggtgtt ttgccttggc gacgctacgc
   124681 ttaatctgtt actgatttct ggtaccatgg ggggaataat ctgattttat ttgattgcaa
   124741 attaaccact taaacctatt gaatgcggaa cattgtcagt cggttaattt tgcgtgcttt
   124801 agcattcaca tctttccgga cgatgcagtg aaaattgcgt aatcctcagc aaccgctgcg
   124861 taatgtcgtc tatcttgtcg cgatcctggc atccctacat tatttgtggt ctgtgaagat
   124921 tatctcattg cagcccctca tcttcgcagg gctggcttta cagcttttca ccctacgtta
   124981 taagaagttc cgtcgatgat ggcgctaatt tcgtgaattg tgcggcttgt tgcaaattac
   125041 acggtgttga aggttattta catgttagct gttgattatc ttccctgata agaccagtat
   125101 ttagctgcca attgctacga aatcgttata atgtgcgacc tcgtcctccc tgacgcagtt
   125161 tttgcgctgc ggaaaaggtg acattggcgc aacgaaggta tattttgttt tttgccggag
   125221 gatagcagca gatcgctgca caatgtccgt caagtctaac attgacactc tggggcaaaa
   125281 tagaccggcg tcccggcctg ctggaattta tcgctatgca tacagctgtc ggggcatacg
   125341 ctttacagac ggcggtgaaa cgcctgtcac aatcacacta aacaaagagt acggaaccca
   125401 ctcatggata ttcgtaagat taaaaaactg atcgagctgg ttgaagaatc aggcatctcc
   125461 gaactggaaa tttctgaagg cgaagagtca gtacgcatta gccgtgcagc tcctgccgca
   125521 agtttccctg tgatgcaaca agcttacgct gcaccaatga tgcagcagcc agctcaatct
   125581 aacgcagccg ctccggcgac cgttccttcc atggaagcgc cagcagcagc ggaaatcagt
   125641 ggtcacatcg tacgttcccc gatggttggt actttctacc gcaccccaag cccggacgca
   125701 aaagcgttca tcgaagtggg tcagaaagtc aacgtgggcg ataccctgtg catcgttgaa
   125761 gccatgaaaa tgatgaacca gatcgaagcg gacaaatccg gtaccgtgaa agcaattctg
   125821 gtcgaaagtg gacaaccggt agaatttgac gagccgctgg tcgtcatcga gtaacgaggc
   125881 gaacatgctg gataaaattg ttattgccaa ccgcggcgag attgcattgc gtattcttcg
   125941 tgcctgtaaa gaactgggca tcaagactgt cgctgtgcac tccagcgcgg atcgcgatct
   126001 aaaacacgta ttactggcag atgaaacggt ctgtatcggc cctgctccgt cagtaaaaag
   126061 ttatctgaac atcccggcaa tcatcagcgc cgctgaaatc accggcgcag tagcaatcca
   126121 tccgggttac ggcttcctct ccgagaacgc caactttgcc gagcaggttg aacgctccgg
   126181 ctttatcttc atcggcccga aagcagaaac cattcgcctg atgggcgaca aagtatccgc
   126241 aatcgcggcg atgaaaaaag cgggcgttcc ttgcgtaccg ggttctgacg gcccgctggg
   126301 cgacgatatg gataaaaacc gcgccattgc taaacgcatt ggttatccgg taattatcaa
   126361 agcctccggc ggcggcggcg gtcgcggtat gcgcgtagtg cgcggcgacg ctgaactggc
   126421 acaatccatc tccatgaccc gtgcggaagc gaaagctgct ttcagcaacg atatggttta
   126481 catggagaaa tacctggaaa atcctcgcca cgtcgagatt caggtactgg ctgacggtca
   126541 gggcaacgct atctatctgg cggaacgtga ctgctccatg cagcgccgcc accagaaagt
   126601 ggtcgaagaa gcaccagcac cgggcattac cccggaactg cgtcgctaca tcggcgaacg
   126661 ttgcgctaaa gcatgtgttg atatcggcta tcgcggtgcg ggtactttcg agttcctgtt
   126721 cgaaaacggc gagttctatt tcatcgaaat gaacacccgt attcaggtag aacacccggt
   126781 tactgaaatg atcaccggcg ttgacctgat caaagaacag ctgcgtatcg ctgccggtca
   126841 accgctgtcg atcaagcaag aagaagttca cgttcgcggc catgcggtgg aatgccgtat
   126901 caacgccgaa gatccgaaca ccttcctgcc aagcccgggc aaaatcaccc gtttccacgc
   126961 accaggcggt tttggcgtgc gttgggagtc tcatatctac gcgggctaca ccgtaccgcc
   127021 gtactatgac tcaatgatcg gtaagctgat ttgctacggt gaaaaccgtg acgtggcgat
   127081 tgcccgcatg aagaatgcgc tgcaggagct gatcatcgac ggtatcaaaa ccaacgttga
   127141 tctgcagatc cgcatcatga atgacgagaa cttccagcat ggtggcacta acatccacta
   127201 tctggagaaa aaactcggtc ttcaggaaaa ataagactgc aaaagcgtca aaaggccgga
   127261 ttttccggcc ttttttatta ctggggatcg acaaccccca taaggtacaa tccccgcttt
   127321 cttcacccat cagggacaaa aaatggacac tcgttttgtt caggcccata aagaggcgcg
   127381 ctgggcgctg gggctgaccc ttttgtatct ggcggtatgg ttagtagccg cttacttacc
   127441 tggcgttgcc cccggtttta ccggttttcc gcgctggttt gagatggcct gcatcctgac
   127501 gccgctgctg tttattggac tgtgctgggc gatggtgaaa tttatctatc gcgatatccc
   127561 actggaggat gacgatgcag cttgaagtaa ttctaccgct ggtcgcctat ctggtggtgg
   127621 tgttcggtat ctctgtttat gcgatgcgta aacggagcac tggcaccttc cttaatgagt
   127681 atttcctcgg cagccgctct atgggcggta ttgtgctggc gatgacgctc accgcgacct
   127741 atatcagtgc cagttcgttt atcggcgggc ctggagctgc ttataaatac ggcctgggct
   127801 gggtattgct ggcgatgatt cagcttcctg cagtctggct ttcgctcggt attctcggca
   127861 agaagttcgc aattcttgcg cgccgctaca atgcggtgac actgaacgac atgctgtttg
   127921 cccgctacca gagtcgtctt ttggtgtggc tggcgagttt gagtttgctg gtcgcgttta
   127981 ttggcgcgat gaccgtgcag tttatcggcg gcgcgcgcct gctggaaacc gcggcgggta
   128041 ttccttatga aaccgggctg ctgattttcg gtatcagcat tgcgttgtat accgcctttg
   128101 gtggctttcg cgccagcgtg ctgaacgaca ctatgcaagg gctggtgatg ctgattggca
   128161 ccattgtgct gcttattggc gtggtacatg ccgcaggtgg cttaagcaac gcagtagaga
   128221 cgttgcaaac catcgatccg caactggtta cgccacaagg cgctgacgat attctgtcgc
   128281 ctgcctttat gacgtcgttc tgggtactgg tgtgctttgg cgtgattggc ctgccgcata
   128341 ctgcggtgcg ctgtatctct tataaagaca gcaaagccgt acatcggggg atcatcatcg
   128401 gtacgattgt ggtcgcaatc ctgatgttcg gtatgcacct ggccggagcg ttaggtcgga
   128461 cggtgatccc cgatctcacc gtaccggacc tggtgatccc gacgttaatg gtaaaagtgt
   128521 tgccaccgtt tgctgccggg atcttcctgg ctgcacctat ggctgcgatc atgtcgacaa
   128581 ttaacgccca actgctgcaa agttccgcta cgatcattaa agatctctat ctgaatatcc
   128641 gtccggatca aatgcaaaac gagacgcgtc tgaagcggat gtcggcggta attacgttag
   128701 ttctcggtgc gttgctgctg cttgccgcct ggaagccgcc agaaatgatc atctggctga
   128761 atttgctggc cttcggtggg ctggaagccg ttttcctgtg gccgctggta ctgggtcttt
   128821 actgggaacg cgccaacgcc aaaggcgcgc taagtgcgat gattgttggc ggcgtactgt
   128881 atgccgtact cgcgacgctg aatattcagt acctgggctt ccaccctatc gtgccctcgt
   128941 tactactaag tttgctggct ttcctggtcg gaaaccgttt cggtacatcc gtcccgcaag
   129001 ctaccgtttt gactactgat aaataaagag ttttgccatg ccttggatcc aactgaaact
   129061 gaacaccacc ggcgcgaacg cggaagatct tagcgatgcg ctgatggaag cgggtgccgt
   129121 ttctatcact tttcaggata cccacgatac gcctgtgttt gagccgctgc cgggcgaaac
   129181 gcgcctgtgg ggcgacaccg atgtgattgg tctgttcgac gctgaaaccg atatgaacga
   129241 cgtggtggcg attctggaaa accatccgct gctcggcgcg ggcttcgcgc ataaaatcga
   129301 acaactggaa gataaagact gggagcgcga atggatggat aatttccacc cgatgcgctt
   129361 tggtgaacgg ctgtggatct gcccaagctg gcgtgatgtg ccggacgaaa acgccgtcaa
   129421 cgtgatgtta gatccagggc tggcgtttgg tacgggtacc catccaacta cctctctgtg
   129481 cctgcagtgg ctcgacagcc tcgatttaac cggtaaaaca gtcatcgact ttggctgcgg
   129541 ttccggcatt ctggctatcg cggcgctgaa actgggtgca gcaaaagcca ttggtattga
   129601 tattgatccg caggcgattc aggccagccg cgacaacgcg gaacgtaatg gcgtctctga
   129661 ccgtctggaa ctctacttac cgaaagatca gccagaagaa atgaaagccg acgtggtggt
   129721 cgctaacatc cttgcaggcc cattacgtga actggcaccg ttaatcagcg tcctgccagt
   129781 ttcaggcggt ttgctgggcc tttccggtat tctggcaagc caggcagaga gcgtttgcga
   129841 agcttatgcc gacagcttcg ctctggaccc ggtcgtggaa aaagaagagt ggtgccgcat
   129901 taccggtcgt aagaattaac cttcgcatcg ccgtagggtg acgcagagcg aagtgcgagc
   129961 gatgtcacaa aaggcacgtt aatttgccga ttatttacgc aaatttgcgt gccaaaattt
   130021 tcattcataa agaaaaattg agaacttact caaatttctt ggagtgtaaa ttttagtcac
   130081 tattttctaa tatgatgatt tttatgagta attatcgcgc cacgctcatt ttaaatgcaa
   130141 ttctttgatc catctcagag gattggtcaa agtttggcct ttcatctcgt gcaaaaaatg
   130201 cgtaatatac gccgccttgc agtcacagta tggtcatttc ttaactcatg cgcatcggac
   130261 aatatcagct cagaaatcgc ctgatcgcag cgcccatggc tggcattaca gacagacctt
   130321 ttcggacgtt gtgctacgag atgggagccg gattgacagt atccgagatg atgtcttcta
   130381 accctcaggt ttgggaaagc gacaaatctc gtttacggat ggtgcacatt gatgaacccg
   130441 gtattcgcac cgtgcaaatt gctggtagcg atccgaaaga aatggcagat gcagcacgta
   130501 ttaacgtgga aagcggtgcc cagattattg atatcaatat gggttgcccg gctaaaaaag
   130561 tgaatcgcaa gctcgcaggt tcagccctct tgcagtaccc ggatgtcgtt aaatcgatcc
   130621 ttaccgaggt cgtcaatgca gtggacgttc ctgttaccct gaaaattcgc actggctggg
   130681 caccggaaca ccgtaactgc gaagagattg cccaactggc tgaagactgt ggcattcagg
   130741 ctctgaccat tcatggccgt acacgcgcct gtttgttcaa tggagaagct gagtacgaca
   130801 gtattcgggc agttaagcag aaagtttcca ttccggttat cgcgaatggc gacattactg
   130861 acccgcttaa agccagagct gtgctcgact atacaggggc ggatgccctg atgataggcc
   130921 gcgcagctca gggaagaccc tggatctttc gggaaatcca gcattatctg gacactgggg
   130981 agttgctgcc cccgctgcct ttggcagagg ttaagcgctt gctttgcgcg cacgttcggg
   131041 aactgcatga cttttatggt ccggcaaaag ggtaccgaat tgcacgtaaa cacgtttcct
   131101 ggtatctcca ggaacacgct ccaaatgacc agtttcggcg cacattcaac gctattgagg
   131161 atgccagcga acagctggag gcgttggagg catacttcga aaattttgcg taaacagaaa
   131221 taaagagctg acagaactat gttcgaacaa cgcgtaaatt ctgacgtact gaccgtttct
   131281 accgttaact ctcaggatca ggtaacccaa aaacccctgc gtgactcggt taaacaggca
   131341 ctgaagaact attttgctca actgaatggt caggatgtga atgacctcta tgagctggta
   131401 ctggctgaag tagaacagcc cctgttggac atggtgatgc aatacacccg tggtaaccag
   131461 acccgtgctg cgctgatgat gggcatcaac cgtggtacgc tgcgtaaaaa attgaaaaaa
   131521 tacggcatga actaattcag gttagctaaa tgcttgatta aaaaggcgct actcggcatg
   131581 gggaagcgcc ttttttatag gtgtcacaaa gggagtgacc atgagaacag gatgtgaacc
   131641 gacccgattt ggtaatgaag caaagaccat catccagggt gatgcattaa ccgaacttaa
   131701 aaagctcccc gccgaaagcg tcgatctgat ctttgccgac ccaccgtata acatcggtaa
   131761 aaattttgat ggtctgatcg aagcctggaa agaagatctg tttatcgact ggctgtttga
   131821 agtgattgca gagtgccacc gcgttctgaa aaagcagggc agcatgtaca tcatgaacag
   131881 cacggaaaac atgcccttta tcgatctcca gtgccgcaag ctttttacca tcaaaagtcg
   131941 catcgtctgg tcatatgaca gttcaggagt gcaggcgaaa aaacactacg gctccatgta
   132001 cgaacccatc ctgatgatgg tgaaagacgc aaagaactac acattcaacg gtgatgctgt
   132061 tctggtagaa gccaaaaccg gatcgcagcg cgcgttgatc gattatcgca aaaatcctcc
   132121 acagccttac aatcatcaaa aagtaccggg taacgtctgg gattttcctc gcgtgcgtta
   132181 tttgatggat gaatatgaaa accacccgac gcaaaaaccg gaagccttac tgaaacgcat
   132241 tattctcgcc tcttccaacc caggcgatat cgttctcgac ccgtttgccg gtagctttac
   132301 taccggtgcc gtagccgtcg ccaacgggcg aaaattcatt ggtattgaga tcaacagcga
   132361 gtacatcaaa atggggcttc gacggctgga tgtcgcgtcg cattactctg cggaagaact
   132421 ggcgaaagtg aaaaaaagaa agaccggcaa cctgtcaaaa cgaagccggt taagcgaagt
   132481 tgaccccgat ctcattgcaa agtaaaggaa gtgtaagcct ggttttcaga ttattcattt
   132541 cgtgtatatt tcctgccaga cttggttaaa catgcacagg ctcaggtaat gattcgcaag
   132601 tattggtggc tcgtcgtttt cgctgtcttc gtttttctgt ttgatacttt actgatgcag
   132661 tggattgaac tgctggcaac agaaacagac aaatgccgca atatgaactc agttaatcca
   132721 ctaaaactgg ttaactgtga cgaactgaat tttcaggaca gaatgtgaat ttatatgaca
   132781 cttaattcat tcgttttatg aattaatttc gttatgtttt catctggcat gaacattctt
   132841 aatacgttat cgaccagagc gggggcttgt ttataaagat cataacccgc catattcatt
   132901 aaccagtttt gaacaattcc gctgaaggca ccatcaataa taatcatcac aacatctaaa
   132961 tcgagattat ttgctacaca accttgttgc tgacacgcct gcaatacttc gcggagagtc
   133021 tgcggattaa agcccatctt ttcgcgtatc actccctcgg ccagcatctc atcattaaat
   133081 tcacatttgt gatataagat tttcagcaac gcctgctggc ggggaatttt ggcaatatat
   133141 tgcaaaccga caatcaattt ttcacgcaat tgctgaaacg ggtcatgctc taacccagcg
   133201 gtcaagtgat cctggattaa ctcccgcaat gaaggctgtt gcaaccacat ctcattaaac
   133261 agttgggtct tgttctcgaa gtgccagtag atagcgccac gcgtaacgtt agcagcgtcg
   133321 gcaatgtcgt tgagcgtcgt cttgcttacg ccatgttgcg caaactgggc gatggcagtt
   133381 tcaatcagtt cttgccgggt cttcagagct tcggctttgg ttctttttgc catgattaat
   133441 tattcaggaa ataaatatat tcgacacaga gtgagaaaat agcgaaggtt aatctatcac
   133501 ctaatgtgta tttatacgag aggctaatat tgagttgcta taaatcgtta aataaataat
   133561 atatattatt tatttaagat acattcacta catcaatata tatttcaatt tacgaggatt
   133621 tgattctacc tctttaaacc cgcgtcaaaa taaaacagta gaatattaat ctttttttgt
   133681 gtttatgtgc cttgagacgc cagtattcat aactattcct tacatcgacg aatgataatt
   133741 tgtaggatag cgaactgtat ttttctttct gcgagttaac gcgttgcctt tttgggtaaa
   133801 taacgcgctt ttggtttttt gaggaatagt aatgacgaaa catgccaggt ttttcctcct
   133861 gccctccttt attctgatct ccgcggcttt aatcgccggt tgtaacgata agggagaaga
   133921 gaaagctcac gtcggtgaac cgcaggttac cgttcatatt gtgaaaacgg ccccgttaga
   133981 agttaagact gaattaccag gccgcaccaa tgcttaccgt atagccgaag ttcgcccaca
   134041 ggttagcggg atcgtactga atcgcaattt cactgaaggc agcgatgtgc aagcaggcca
   134101 gtccctgtac cagatcgatc ccgcgaccta tcaggcaagt tatgacagcg cgaaaggcga
   134161 actagcgaaa agtgaagccg ccgccgccat cgcgcatttg acggtaaaac gttacgttcc
   134221 gctcgtgggt acgaaataca tcagtcagca ggagtacgac caggccattg ctgatgctcg
   134281 ccaggccgat gccgccgtga ttgccgcaaa agctacagtt gaaagcgctc gcatcaatct
   134341 cgcttatacc aaagtgactg cgcctattag cggacgtatc ggcaaatcga ctgtgaccga
   134401 aggcgctctt gtcactaatg ggcaaacgac tgaactggcg actgtccagc agctcgatcc
   134461 tatctacgtt gatgtgaccc aatccagcaa cgattttatg aggctgaagc aatccgtaga
   134521 gcaaggaaat ttgcataagg aaaacgccac cagcaacgta gagttggtca tggagaacgg
   134581 tcaaacctat cccctgaaag gtacgctgca attctccgat gtgaccgttg atgaaagcac
   134641 cggctccata accctacgtg ctgtcttccc taacccgcaa catacgcttt tgccgggtat
   134701 gtttgtgcgc gcacggattg atgaaggcgt ccaacctgac gccattctta tcccgcaaca
   134761 aggcgttagc cgcacaccac gtggtgatgc aaccgtgctg attgttaaca ataaaagtca
   134821 ggttgaagcg cgccctgtcg ttgccagcca ggcgattggc gataaatggt tgattagcga
   134881 agggctgaaa tctggcgatc aagtcattgt cagcggccta caaaaagcgc gtccgggaga
   134941 gcaggttaaa gccactaccg atacccccgc agatactgca tcgaagtaag gcaatctgac
   135001 atggcaaact tttttattcg acgaccgata tttgcatggg tgctggccat tattctgatg
   135061 atggcgggcg cactggcgat cctacaattg cccgtcgctc agtatccaac cattgcaccg
   135121 cctgcggttt ctgtttcagc aaactatccg ggcgctgatg cgcagaccgt gcaggatacg
   135181 gtgacgcagg ttatcgaaca gaatatgaac ggtatcgata acctgatgta tatgtcctcc
   135241 accagcgatt ccgccggtag cgtgacaatt acccttacct tccagtccgg gaccgatcct
   135301 gatatcgcgc aagtgcaggt gcagaacaaa ctccagctcg ccacgccgtt gctgccgcag
   135361 gaagtacagc agcaggggat cagtgttgaa aagtccagta gcagctattt gatggtggcg
   135421 ggctttgtct ctgataaccc aggcaccaca caggacgata tctcggacta tgtggcctct
   135481 aacgttaaag atacgcttag ccgtctgaat ggcgtcggtg acgtacagct tttcggcgca
   135541 cagtatgcga tgcgtatctg gctggacgcc gatctgctaa acaaatataa actgacaccg
   135601 gttgatgtga ttaaccagtt gaaggtacag aacgatcaga tcgctgccgg acagttgggc
   135661 ggaacgccag cgttaccagg gcaacagttg aacgcctcga ttattgctca gacgcggtta
   135721 aaaaatccgg aagaattcgg caaagtgacc ctgcgcgtaa acagtgacgg ctcggtggta
   135781 cgcctgaaag atgtcgcacg ggttgaactt ggcggtgaaa actacaacgt tatcgctcgt
   135841 atcaacggaa aaccggcggc gggcctgggg attaagctgg caaccggcgc aaatgctctc
   135901 gataccgcga aagccattaa ggcaaaactg gcggaattac agccattctt cccgcaggga
   135961 atgaaggttc tctaccctta tgacaccaca ccattcgtcc agctttctat tcacgaagtg
   136021 gtaaaaacgc tgttcgaagc cattatgctg gtgttcctgg tgatgtatct gttcttgcag
   136081 aatatgcgag caacgctgat ccccaccatt gcggtacccg tggtgttgtt agggacgttt
   136141 gccatcctcg ccgcttttgg ttactccatc aacacactaa cgatgttcgg gatggtgctt
   136201 gccatcgggc tgctcgtcga tgatgcgata gtggtggtgg agaacgtcga gcgcgtgatg
   136261 atggaggata agctcccgcc aaaagaagcg acggaaaaat caatgtcgca aatccagggc
   136321 gcactggtgg gtatcgcaat ggtgctgtca gcggtattta ttccgatggc attcttcggc
   136381 ggttctactg gggcaattta tcgtcagttc tctatcacca tcgtttcggc aatggcgctt
   136441 tctgttctgg tggcattgat tcttacccct gcgttatgtg caacgctgct taaacccgtc
   136501 tctgctgagc atcacgaaaa taagggcggt ttcttcggct ggtttaatac caccttcgat
   136561 catagcgtta accactacac caacagcgtc ggcaaaatcc tcggttccac aggacgatat
   136621 ttactgatct atgcactaat tgttgcagga atggtggtgt tgtttttacg tcttccgtct
   136681 tccttcttac ctgaagagga tcagggtgtc tttctgacta tgattcagtt acccgctggc
   136741 gcgacgcaag agcggacgca aaaagtactg gatcaagtga cggattacta tctgaagaac
   136801 gagaaagcga acgttgaaag tgtctttaca gttaacggct ttagcttcag cggccaggca
   136861 caaaacgccg gtatggcttt cgtcagtctg aaaccgtggg aagagcgtag tggtgacgaa
   136921 aacagtgcgg aagcggtaat ccatcgtgcc aaaatggaat tgggcaagat ccgcgacggt
   136981 tttgtcattc cattcaatat gccagccatt gttgaactgg gcacggcaac gggtttcgac
   137041 tttgagttaa ttgatcaggc tgggctgggt catgatgccc tgacccaggc ccgtaaccag
   137101 ttgcttggca tggcggcgca acatcctgcc agcttagtca gcgtgcgtcc taatggtctg
   137161 gaagacaccg cgcagtttaa actggaagtt gaccaagaaa aggcgcaggc attaggtgtt
   137221 tcactttctg acatcaatca gaccatttca acagcgttgg gtgggactta cgttaacgac
   137281 ttcattgacc gtggtcgcgt aaaaaaggtg tatgttcagg cggatgccaa atttcgtatg
   137341 ctgccagaag atgtcgataa actttatgtc cgtagcgcca atggcgaaat ggtgccattc
   137401 tctgccttta ccactgcaca ttgggtgtat ggttctccgc gactggagcg ctacaacggt
   137461 ctgccgtcaa tggagattca gggggaagcc gcgccaggaa ccagttccgg cgatgccatg
   137521 gcgttgatgg aaaaccttgc atcacaatta ccagctggta ttggttatga ctggacgggt
   137581 atgtcatatc aggaacgctt atcgggaaac caggctcccg ctctggtagc aatttccttt
   137641 gtggttgttt tcctgtgcct tgctgcactc tatgaaagct ggtcaattcc tgtctcggtt
   137701 atgttggtcg tgccgttagg gattgtcggc gtgctactgg cggcgacact ctttaatcaa
   137761 aaaaatgacg tctactttat ggtgggcttg ctaacgacaa ttggcttgtc ggctaaaaac
   137821 gctatattga ttgttgagtt cgctaaagat ctcatggaga aagagggtaa aggtgttgtt
   137881 gaagcgacac taatggcagt acgtatgcgt ctgcgtccta tcctgatgac ctctctcgcc
   137941 tttattctcg gcgtattacc gctagctatc agtaacggtg ccggcagtgg cgcgcagaac
   138001 gcagtgggta tcggggtaat gggcgggatg gtttctgcaa cgttgctggc aatcttcttc
   138061 gtgccggtgt tctttgtggt gatccgccgt tgctttaaag gataaatcag aaacataaag
   138121 gcactttcgg gtgcctttat tatttccagt gaaacccata aaaattaata agatattcct
   138181 ctactcactc tttaaaaact ttctatagtt cccgctccct tcactatttt tacaattcac
   138241 ataatttgtc attattcatt ccgcaagatt tataacctcc agtgataaaa tggcattcag
   138301 ctcgttaata agagagttaa cttattaagc gttagcgttt attactgagg taacaccatg
   138361 aaaagattaa ttcctgtggc attgctcacc gcattgctgg cgggctgtgc gcacgattcg
   138421 ccctgcgttc cggtttatga cgatcagggt cgtctggttc acaccaatac ctgtatgaaa
   138481 ggcacgactc aggataactg ggaaacggct ggggctattg ccggcggggc agcagctgtt
   138541 gctggcctga cgatggggat catcgctctg tcgaaataac taaacagggc gcggagctcc
   138601 gcgctctttt taacgataca gtatctttcc ataagataaa atattgctat attctgctgg
   138661 gtaattccct gctgatttag catgttatat cacgttattt cactgctttc gaaataacgc
   138721 atcaatttaa ctttcacaca tcccaccaga aatatactca cgctaacaat cattattctg
   138781 cttagtaaaa aacgcgtaaa tattttgcgc caaaatgtgg cgcatgtttc attttcgcac
   138841 cattgcgggg cgcagttttt attatttcct gtctacactc agcttattgc gacgcgaaat
   138901 cgtgcgcaaa agctggcact acttttgctt ataagaggat ggccacagac aggtaaaaga
   138961 cgtttcccaa acgtcctata acgataattt tcgcctcaca ggaagcatta tgaaaaagat
   139021 gatgatagcc acactggctg ccgccagcgt gctgcttgcc gttgcaaatc aggcgcatgc
   139081 tggcgcgacg cttgatgccg ttcagaaaaa aggttttgtg caatgcggga tcagtgatgg
   139141 attacctggg ttctcttatg ccgatgctga cggtaagttt tcaggtattg atgtagatgt
   139201 ttgtcgtggt gttgccgctg ctgtattcgg tgacgatacg aaagtgaaat ataccccgct
   139261 cactgcaaaa gaacgcttca ccgctttaca gtcaggggag gtggatttgc tctcccgtaa
   139321 tacgacctgg acttcatctc gcgatgccgg gatgggaatg gcatttactg gcgtcactta
   139381 ttacgacggc attggcttcc tgacgcacga taaagcgggg ctaaaaagcg cgaaagaact
   139441 ggatggcgct accgtctgta ttcaggcggg tactgatacc gaactcaacg ttgccgatta
   139501 ctttaaggca aacaatatga agtacacacc agtgactttc gatcgctctg acgaatcagc
   139561 gaaggcactg gaatctggtc gctgcgatac gctggcctcg gatcaatcac aactgtatgc
   139621 cctgcgcatc aaattaagca acccggctga atggattgtc ttaccggaag ttatctctaa
   139681 agaaccactt gggccggtag ttcgtcgtgg cgatgatgaa tggttctcga ttgtacgctg
   139741 gacgcttttc gccatgctga atgctgaaga gatgggcatc aattcccaga acgtcgatga
   139801 aaaagcggct aatccagcaa cacctgatat ggcacatctg ctgggtaaag aaggcgatta
   139861 cggcaaggat ctgaagctgg ataataaatg ggcctttaac atcatcaaac aggtgggtaa
   139921 ctactcggaa attttcgagc gtaacgtagg ttcagaaagc ccgctgaaaa ttaaacgtgg
   139981 gcaaaataat ctctggaata acggcggtat tcagtacgcg ccgcccgtgc gttaagtcgg
   140041 tgtgaggtaa cgagcgtcgc tacggcggcg ctctcttcag cgttatgctt tccgaggttt
   140101 ctatgtctca tcgccgctca accgttaaag gctcactctc ctttgccaac cctacggttc
   140161 gcgcctggtt attccagatc tttgccgttg ttgctgttgt cggaattgtt ggttggctat
   140221 ttcacaacac tgtgacgaat ctcagtaatc gtggcattac ttcaggtttt gcctttctgg
   140281 atcgcggcgc tggcttcggt attgtccagc atttgatcga ttaccagcag ggtgatacct
   140341 acggacgcgt tttcattgtt ggcttactca atacgctact ggtttctgca ttgtgtattg
   140401 tattcgcttc tgttctgggc ttctttatcg gtctggcgcg actttcggat aactggctgc
   140461 tacgaaagct ttccacaatt tatattgaga tcttccgtaa tattccgccg ctgctgcaaa
   140521 tcttcttctg atactttgcc gtgttgcgca atttgcccgg accacgccag gcagtgagtg
   140581 cgtttgatct ggcctttttg agcaatcgtg ggctttatat tccatctccg cagctgggag
   140641 acggatttat tgcgtttatc gtgactgttg ttattgctat agccatttct gttggactat
   140701 tccgttttaa taaaacgtac cagataaaga ccggacaatt gcgccgaacc tggccggtcg
   140761 ccgttatgtt gatcataggt ttgcctttac tggcgcaatg gctttttggc gcagcactac
   140821 actgggatgt tccagcccta cgaggcttta acttccgtgg cgggatggtt ttaattcctg
   140881 aactggcagc cttaacgctg gcgctttcgg tttatacctc tgcatttatc gccgagatta
   140941 tccgcgctgg gatccaggca gtgccttatg gtcaacatga agcggctcgg tcactgggat
   141001 tacccaatcc ggttacgcta cgccaggtca ttattcccca ggcactgcga gtgattattc
   141061 cgccgttaac cagccagtat ctcaacatcg tcaaaaactc ctctcttgcc gccgctattg
   141121 gctatccgga tatggtttcg ctgtttgctg gcaccgtgct gaaccagacg gggcaagcca
   141181 tcgagacgat agccatgacc atgtcggtct atctgattat cagcctgact atctcgctgc
   141241 tgatgaatat ctataaccgc cgcatcgcga tcgttgaacg ctaaggagtc atgatgacaa
   141301 aagtattgct gtctcatccc ccgcgcccgg cgagccataa ctcaagccgc gcgatggtgt
   141361 gggtgcgaaa aaatctgttc tccagctgga gcaatagcct gctgactatt ggctgcatat
   141421 ggttgatgtg ggaactgatt ccaccgttgc tgaactgggc atttttgcag gctaactggg
   141481 ttggctcaac gcgtaccgac tgcacaaaag ccggtgcctg ttgggtcttc atccacgaac
   141541 gatttggtca gtttatgtat gggctttacc cacacgacca acgctggcga attaacctcg
   141601 cattactgat tgggcttgtg tcgatcgcac caatgttctg gaaaatactc ccgcatcgcg
   141661 gtcgctatat tgcggtatgg gcggtgattt acccactgat tgtctggtgg ctgatgtatg
   141721 gcgggtttct tggtcttgag cgggttgaaa cccggcaatg gggcgggctg acgctaactt
   141781 taattattgc atcagttggg attgcggggg cgctgccgtg ggggatctta ctggcgttag
   141841 gtcgccgctc ccatatgccg attgtgcgta tcttatcggt catttttatc gagttctggc
   141901 gcggtgtacc gctgataacc gttctgttta tgtcttcggt catgctgccg ttgtttatgg
   141961 cagaaggcac cagtatcgac aaattgatcc gcgcgctggt tggcgtgatc ctgtttcagt
   142021 cagcatatgt tgcggaagtc gtgcgaggcg gattacaggc gctgcctaaa gggcaatatg
   142081 aagcggcaga gtcgctggcg ttgggttact ggaaaaacca ggggctggtt attctgccac
   142141 aggcgttgaa gctggtgatt cctgggctgg taaataccat catcgcactc ttcaaagata
   142201 ccagcctggt gatcatcatc gggttgttcg atcttttcag tagcgttcag caggcaaccg
   142261 ttgatcccgc ctggttgggt atgtcgacgg aagggtatgt tttcgccgca ctgatctact
   142321 ggatcttctg tttcagcatg tcacgttata gccaacatct ggaaaaacgt tttaacaccg
   142381 ggcgtacacc gcattgagga cactatgagc caaattttac tgcaacctgc taacgcgatg
   142441 attacgctgg aaaacgtcaa taaatggtat gggcaattcc atgttttgaa aaatataaat
   142501 ttaaccgtgc aaccgggaga acggatcgtt ctgtgtggcc cttctggttc cggtaaatcg
   142561 acaaccattc gttgtattaa tcatctggaa gaacaccaac agggacggat cgtggtagat
   142621 ggcatcgagc ttaacgaaga tattcgcaat attgagcgcg tcaggcagga agtgggaatg
   142681 gtctttcagc atttcaatct cttccctcat ctgaccgttc tacagaactg taccctggca
   142741 ccgatttggg tacgcaagat gccaaagaaa gaggcagaag ctctggcgat gcattacctt
   142801 gagcgggtga gaattgccga acatgcgcat aagtttcccg gacagatttc aggtggtcag
   142861 cagcaacgcg ttgccattgc gcgttcgctg tgtatgaagc cgaaaattat gttatttgat
   142921 gagccaacgt cggcgctcga tcctgagatg gtgaaagagg tgctggatac gatgattggg
   142981 ctggcgcagt cgggtatgac aatgctgtgt gtaacacatg agatggggtt tgcacgaacc
   143041 gtcgctgaca gggtgatttt tatggatcgt ggggagatag tggaacaagc tgcaccggat
   143101 gagttttttg cgcatcctaa atcagagcgt acgagggcat ttttatcgca ggtgatccat
   143161 taattgaatg ttagttcgaa aagcaaaaag gccatccttt cggatggcct ttcgcttgat
   143221 ttgatgtctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
   143281 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
   143341 acaaattctt ttctaatctg ccgaacttta acctaaaaag tggtgctgat acccagagtc
   143401 gaactgggga cctcaccctt accaagggtg cgctctacca actgagccat atcagca
//
LOCUS       DAZGTJ010000002       123457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000002 DAZGTJ010000000
VERSION     DAZGTJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 123457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.002"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(271..1128)
                     /gene="murI"
                     /locus_tag="ACHK8E_000146"
     CDS             complement(271..1128)
                     /gene="murI"
                     /locus_tag="ACHK8E_000146"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP0342474.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1073..2956)
                     /gene="btuB"
                     /locus_tag="ACHK8E_000147"
     CDS             complement(1073..2956)
                     /gene="btuB"
                     /locus_tag="ACHK8E_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0342475.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     regulatory      complement(3021..3211)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3325..4425
                     /gene="trmA"
                     /locus_tag="ACHK8E_000148"
     CDS             3325..4425
                     /gene="trmA"
                     /locus_tag="ACHK8E_000148"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP0342476.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4465..4824)
                     /gene="yijD"
                     /locus_tag="ACHK8E_000149"
     CDS             complement(4465..4824)
                     /gene="yijD"
                     /locus_tag="ACHK8E_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP0342477.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4824..5474)
                     /gene="fabR"
                     /locus_tag="ACHK8E_000150"
     CDS             complement(4824..5474)
                     /gene="fabR"
                     /locus_tag="ACHK8E_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP0342478.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5806..7206
                     /gene="sthA"
                     /locus_tag="ACHK8E_000151"
     CDS             5806..7206
                     /gene="sthA"
                     /locus_tag="ACHK8E_000151"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP0342479.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7189..8106)
                     /gene="oxyR"
                     /locus_tag="ACHK8E_000152"
     CDS             complement(7189..8106)
                     /gene="oxyR"
                     /locus_tag="ACHK8E_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP0342480.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8358..9641)
                     /locus_tag="ACHK8E_000153"
     CDS             complement(8358..9641)
                     /locus_tag="ACHK8E_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0342481.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            complement(9708..10922)
                     /gene="manD"
                     /locus_tag="ACHK8E_000154"
     CDS             complement(9708..10922)
                     /gene="manD"
                     /locus_tag="ACHK8E_000154"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFP0342482.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            complement(11420..12793)
                     /gene="argH"
                     /locus_tag="ACHK8E_000155"
     CDS             complement(11420..12793)
                     /gene="argH"
                     /locus_tag="ACHK8E_000155"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP0342483.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(12854..13630)
                     /gene="argB"
                     /locus_tag="ACHK8E_000156"
     CDS             complement(12854..13630)
                     /gene="argB"
                     /locus_tag="ACHK8E_000156"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP0342484.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(13638..14642)
                     /gene="argC"
                     /locus_tag="ACHK8E_000157"
     CDS             complement(13638..14642)
                     /gene="argC"
                     /locus_tag="ACHK8E_000157"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP0342485.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            14796..15947
                     /gene="argE"
                     /locus_tag="ACHK8E_000158"
     CDS             14796..15947
                     /gene="argE"
                     /locus_tag="ACHK8E_000158"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP0342486.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16249..18900
                     /gene="ppc"
                     /locus_tag="ACHK8E_000159"
     CDS             16249..18900
                     /gene="ppc"
                     /locus_tag="ACHK8E_000159"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP0342487.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            19083..20816
                     /gene="eptC"
                     /locus_tag="ACHK8E_000160"
     CDS             19083..20816
                     /gene="eptC"
                     /locus_tag="ACHK8E_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0342488.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            21042..21893
                     /gene="yijO"
                     /locus_tag="ACHK8E_000161"
     CDS             21042..21893
                     /gene="yijO"
                     /locus_tag="ACHK8E_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0342489.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(21880..22221)
                     /gene="frwD"
                     /locus_tag="ACHK8E_000162"
     CDS             complement(21880..22221)
                     /gene="frwD"
                     /locus_tag="ACHK8E_000162"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0342490.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22223..23101)
                     /gene="pflC"
                     /locus_tag="ACHK8E_000163"
     CDS             complement(22223..23101)
                     /gene="pflC"
                     /locus_tag="ACHK8E_000163"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP0342491.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(23067..25364)
                     /gene="pflD"
                     /locus_tag="ACHK8E_000164"
     CDS             complement(23067..25364)
                     /gene="pflD"
                     /locus_tag="ACHK8E_000164"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0342492.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDMPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(25415..25735)
                     /gene="frwB"
                     /locus_tag="ACHK8E_000165"
     CDS             complement(25415..25735)
                     /gene="frwB"
                     /locus_tag="ACHK8E_000165"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0342493.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(25750..26829)
                     /gene="frwC"
                     /locus_tag="ACHK8E_000166"
     CDS             complement(25750..26829)
                     /gene="frwC"
                     /locus_tag="ACHK8E_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP0342494.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            27138..29639
                     /gene="ptsP"
                     /locus_tag="ACHK8E_000167"
     CDS             27138..29639
                     /gene="ptsP"
                     /locus_tag="ACHK8E_000167"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0342495.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            29651..30313
                     /gene="fsa"
                     /locus_tag="ACHK8E_000168"
     CDS             29651..30313
                     /gene="fsa"
                     /locus_tag="ACHK8E_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0342496.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            30324..31427
                     /gene="gldA"
                     /locus_tag="ACHK8E_000169"
     CDS             30324..31427
                     /gene="gldA"
                     /locus_tag="ACHK8E_000169"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP0342497.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            31817..32320
                     /locus_tag="ACHK8E_000170"
     CDS             31817..32320
                     /locus_tag="ACHK8E_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP0342498.1"
                     /translation="MADGARQQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRAL
                     RSQKVDLQKLVHEDMAKNFAAYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSK
                     NPSDYQAGDIVSWRLDNGLAHIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVG
                     HYRYFAK"
     gene            complement(32347..33252)
                     /gene="yijE"
                     /locus_tag="ACHK8E_000171"
     CDS             complement(32347..33252)
                     /gene="yijE"
                     /locus_tag="ACHK8E_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP0342499.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(33346..35526)
                     /gene="katG"
                     /locus_tag="ACHK8E_000172"
     CDS             complement(33346..35526)
                     /gene="katG"
                     /locus_tag="ACHK8E_000172"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP0342500.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(35855..36745)
                     /gene="metF"
                     /locus_tag="ACHK8E_000173"
     CDS             complement(35855..36745)
                     /gene="metF"
                     /locus_tag="ACHK8E_000173"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP0342501.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(37127..38680)
                     /locus_tag="ACHK8E_000174"
     CDS             complement(37127..38680)
                     /locus_tag="ACHK8E_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0342502.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            38813..39937
                     /locus_tag="ACHK8E_000175"
     CDS             38813..39937
                     /locus_tag="ACHK8E_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFP0342503.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            40170..41720
                     /locus_tag="ACHK8E_000176"
     CDS             40170..41720
                     /locus_tag="ACHK8E_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0342504.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            41761..43380
                     /locus_tag="ACHK8E_000177"
     CDS             41761..43380
                     /locus_tag="ACHK8E_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0342505.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRVDVSK"
     gene            43459..44319
                     /locus_tag="ACHK8E_000178"
     CDS             43459..44319
                     /locus_tag="ACHK8E_000178"
                     /inference="COORDINATES: protein motif:HMM:NF011686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0342506.1"
                     /translation="MCIKKHGIALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSR
                     FGPRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPR
                     FSIDKLTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSA
                     NIYAKYQWQNYGAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKS
                     HNNKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGW
                     GTYTTIGYQF"
     gene            complement(44536..46968)
                     /gene="metL"
                     /locus_tag="ACHK8E_000179"
     CDS             complement(44536..46968)
                     /gene="metL"
                     /locus_tag="ACHK8E_000179"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP0342507.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(46971..48131)
                     /gene="metB"
                     /locus_tag="ACHK8E_000180"
     CDS             complement(46971..48131)
                     /gene="metB"
                     /locus_tag="ACHK8E_000180"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP0342508.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            48408..48725
                     /gene="metJ"
                     /locus_tag="ACHK8E_000181"
     CDS             48408..48725
                     /gene="metJ"
                     /locus_tag="ACHK8E_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP0342509.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            48785..49393
                     /gene="yiiX"
                     /locus_tag="ACHK8E_000182"
     CDS             48785..49393
                     /gene="yiiX"
                     /locus_tag="ACHK8E_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP0342510.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(49454..49666)
                     /gene="rpmE"
                     /locus_tag="ACHK8E_000183"
     CDS             complement(49454..49666)
                     /gene="rpmE"
                     /locus_tag="ACHK8E_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0342511.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            49869..52067
                     /gene="priA"
                     /locus_tag="ACHK8E_000184"
     CDS             49869..52067
                     /gene="priA"
                     /locus_tag="ACHK8E_000184"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP0342512.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            52223..53248
                     /gene="cytR"
                     /locus_tag="ACHK8E_000185"
     CDS             52223..53248
                     /gene="cytR"
                     /locus_tag="ACHK8E_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP0342513.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            53340..54299
                     /gene="ftsN"
                     /locus_tag="ACHK8E_000186"
     CDS             53340..54299
                     /gene="ftsN"
                     /locus_tag="ACHK8E_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP0342514.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            54392..54922
                     /gene="hslV"
                     /locus_tag="ACHK8E_000187"
     CDS             54392..54922
                     /gene="hslV"
                     /locus_tag="ACHK8E_000187"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP0342515.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            54932..56263
                     /gene="hslU"
                     /locus_tag="ACHK8E_000188"
     CDS             54932..56263
                     /gene="hslU"
                     /locus_tag="ACHK8E_000188"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP0342516.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            56330..57256
                     /gene="menA"
                     /locus_tag="ACHK8E_000189"
     CDS             56330..57256
                     /gene="menA"
                     /locus_tag="ACHK8E_000189"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP0342517.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            57349..57834
                     /gene="rraA"
                     /locus_tag="ACHK8E_000190"
     CDS             57349..57834
                     /gene="rraA"
                     /locus_tag="ACHK8E_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP0342518.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(57919..58164)
                     /gene="zapB"
                     /locus_tag="ACHK8E_000191"
     CDS             complement(57919..58164)
                     /gene="zapB"
                     /locus_tag="ACHK8E_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP0342519.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            58590..59435
                     /gene="glpF"
                     /locus_tag="ACHK8E_000192"
     CDS             58590..59435
                     /gene="glpF"
                     /locus_tag="ACHK8E_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP0342520.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            59458..60966
                     /gene="glpK"
                     /locus_tag="ACHK8E_000193"
     CDS             59458..60966
                     /gene="glpK"
                     /locus_tag="ACHK8E_000193"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP0342521.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            61137..62147
                     /gene="glpX"
                     /locus_tag="ACHK8E_000194"
     CDS             61137..62147
                     /gene="glpX"
                     /locus_tag="ACHK8E_000194"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0342522.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            62244..62990
                     /gene="fpr"
                     /locus_tag="ACHK8E_000195"
     CDS             62244..62990
                     /gene="fpr"
                     /locus_tag="ACHK8E_000195"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP0342523.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(62995..63423)
                     /gene="uspD"
                     /locus_tag="ACHK8E_000196"
     CDS             complement(62995..63423)
                     /gene="uspD"
                     /locus_tag="ACHK8E_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP0342524.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(63450..63749)
                     /gene="yiiS"
                     /locus_tag="ACHK8E_000197"
     CDS             complement(63450..63749)
                     /gene="yiiS"
                     /locus_tag="ACHK8E_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP0342525.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(63961..64401)
                     /gene="yiiR"
                     /locus_tag="ACHK8E_000198"
     CDS             complement(63961..64401)
                     /gene="yiiR"
                     /locus_tag="ACHK8E_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0342526.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            64502..65101
                     /gene="yiiQ"
                     /locus_tag="ACHK8E_000199"
     CDS             64502..65101
                     /gene="yiiQ"
                     /locus_tag="ACHK8E_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP0342527.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            65209..65976
                     /gene="tpiA"
                     /locus_tag="ACHK8E_000200"
     CDS             65209..65976
                     /gene="tpiA"
                     /locus_tag="ACHK8E_000200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP0342528.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(66031..66786)
                     /gene="cdh"
                     /locus_tag="ACHK8E_000201"
     CDS             complement(66031..66786)
                     /gene="cdh"
                     /locus_tag="ACHK8E_000201"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP0342529.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(66892..67881)
                     /gene="sbp"
                     /locus_tag="ACHK8E_000202"
     CDS             complement(66892..67881)
                     /gene="sbp"
                     /locus_tag="ACHK8E_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP0342530.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(68201..69163)
                     /gene="pfkA"
                     /locus_tag="ACHK8E_000203"
     CDS             complement(68201..69163)
                     /gene="pfkA"
                     /locus_tag="ACHK8E_000203"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP0342531.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(69344..70246)
                     /gene="fieF"
                     /locus_tag="ACHK8E_000204"
     CDS             complement(69344..70246)
                     /gene="fieF"
                     /locus_tag="ACHK8E_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP0342532.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(70395..70895)
                     /gene="cpxP"
                     /locus_tag="ACHK8E_000205"
     CDS             complement(70395..70895)
                     /gene="cpxP"
                     /locus_tag="ACHK8E_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP0342533.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            71045..71743
                     /gene="cpxR"
                     /locus_tag="ACHK8E_000206"
     CDS             71045..71743
                     /gene="cpxR"
                     /locus_tag="ACHK8E_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP0342534.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            71740..73113
                     /gene="cpxA"
                     /locus_tag="ACHK8E_000207"
     CDS             71740..73113
                     /gene="cpxA"
                     /locus_tag="ACHK8E_000207"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP0342535.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(73219..73893)
                     /gene="yiiM"
                     /locus_tag="ACHK8E_000208"
     CDS             complement(73219..73893)
                     /gene="yiiM"
                     /locus_tag="ACHK8E_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP0342536.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(74042..75025)
                     /gene="kdgT"
                     /locus_tag="ACHK8E_000209"
     CDS             complement(74042..75025)
                     /gene="kdgT"
                     /locus_tag="ACHK8E_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP0342537.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(75285..75905)
                     /gene="sodA"
                     /locus_tag="ACHK8E_000210"
     CDS             complement(75285..75905)
                     /gene="sodA"
                     /locus_tag="ACHK8E_000210"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP0342538.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            76190..77224
                     /gene="rhaT"
                     /locus_tag="ACHK8E_000211"
     CDS             76190..77224
                     /gene="rhaT"
                     /locus_tag="ACHK8E_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP0342539.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(77221..78069)
                     /gene="rhaR"
                     /locus_tag="ACHK8E_000212"
     CDS             complement(77221..78069)
                     /gene="rhaR"
                     /locus_tag="ACHK8E_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP0342540.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(78143..78979)
                     /gene="rhaS"
                     /locus_tag="ACHK8E_000213"
     CDS             complement(78143..78979)
                     /gene="rhaS"
                     /locus_tag="ACHK8E_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP0342541.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            79267..80736
                     /gene="rhaB"
                     /locus_tag="ACHK8E_000214"
     CDS             79267..80736
                     /gene="rhaB"
                     /locus_tag="ACHK8E_000214"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP0342542.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            80733..81992
                     /gene="rhaA"
                     /locus_tag="ACHK8E_000215"
     CDS             80733..81992
                     /gene="rhaA"
                     /locus_tag="ACHK8E_000215"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP0342543.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            82177..83001
                     /gene="rhaD"
                     /locus_tag="ACHK8E_000216"
     CDS             82177..83001
                     /gene="rhaD"
                     /locus_tag="ACHK8E_000216"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP0342544.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPNGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            83011..83325
                     /gene="rhaM"
                     /locus_tag="ACHK8E_000217"
     CDS             83011..83325
                     /gene="rhaM"
                     /locus_tag="ACHK8E_000217"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP0342545.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(83366..84760)
                     /locus_tag="ACHK8E_000218"
     CDS             complement(83366..84760)
                     /locus_tag="ACHK8E_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP0342546.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(85114..85804)
                     /locus_tag="ACHK8E_000219"
                     /pseudo
     CDS             complement(85114..85804)
                     /locus_tag="ACHK8E_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(85893..86726)
                     /gene="fdhD"
                     /locus_tag="ACHK8E_000220"
     CDS             complement(85893..86726)
                     /gene="fdhD"
                     /locus_tag="ACHK8E_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP0342547.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            86920..89970
                     /gene="fdnG"
                     /locus_tag="ACHK8E_000221"
     CDS             86920..89970
                     /gene="fdnG"
                     /locus_tag="ACHK8E_000221"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:87505..87507,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0342548.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            89983..90885
                     /gene="fdoH"
                     /locus_tag="ACHK8E_000222"
     CDS             89983..90885
                     /gene="fdoH"
                     /locus_tag="ACHK8E_000222"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP0342549.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            90882..91517
                     /gene="fdoI"
                     /locus_tag="ACHK8E_000223"
     CDS             90882..91517
                     /gene="fdoI"
                     /locus_tag="ACHK8E_000223"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0342550.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            91514..92443
                     /gene="fdhE"
                     /locus_tag="ACHK8E_000224"
     CDS             91514..92443
                     /gene="fdhE"
                     /locus_tag="ACHK8E_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP0342551.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(92658..92876)
                     /gene="yiiE"
                     /locus_tag="ACHK8E_000225"
     CDS             complement(92658..92876)
                     /gene="yiiE"
                     /locus_tag="ACHK8E_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0342552.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(93481..93771)
                     /gene="nadS"
                     /locus_tag="ACHK8E_000226"
     CDS             complement(93481..93771)
                     /gene="nadS"
                     /locus_tag="ACHK8E_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0342553.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(93772..94083)
                     /locus_tag="ACHK8E_000227"
     CDS             complement(93772..94083)
                     /locus_tag="ACHK8E_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0342554.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            94312..95220
                     /locus_tag="ACHK8E_000228"
     CDS             94312..95220
                     /locus_tag="ACHK8E_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0342555.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(95284..96225)
                     /gene="fabY"
                     /locus_tag="ACHK8E_000229"
                     /gene_synonym="yiiD"
     CDS             complement(95284..96225)
                     /gene="fabY"
                     /locus_tag="ACHK8E_000229"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP0342556.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(96270..96707)
                     /gene="dtd"
                     /locus_tag="ACHK8E_000230"
     CDS             complement(96270..96707)
                     /gene="dtd"
                     /locus_tag="ACHK8E_000230"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP0342557.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(96704..97576)
                     /gene="yihY"
                     /locus_tag="ACHK8E_000231"
     CDS             complement(96704..97576)
                     /gene="yihY"
                     /locus_tag="ACHK8E_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP0342558.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(97570..98169)
                     /gene="yihX"
                     /locus_tag="ACHK8E_000232"
     CDS             complement(97570..98169)
                     /gene="yihX"
                     /locus_tag="ACHK8E_000232"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP0342559.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(98310..99197)
                     /locus_tag="ACHK8E_000233"
     CDS             complement(98310..99197)
                     /locus_tag="ACHK8E_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0342560.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(99187..100536)
                     /locus_tag="ACHK8E_000234"
     CDS             complement(99187..100536)
                     /locus_tag="ACHK8E_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0342561.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(100635..102068)
                     /locus_tag="ACHK8E_000235"
     CDS             complement(100635..102068)
                     /locus_tag="ACHK8E_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0342562.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(102138..103148)
                     /locus_tag="ACHK8E_000236"
     CDS             complement(102138..103148)
                     /locus_tag="ACHK8E_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP0342563.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(103181..104068)
                     /locus_tag="ACHK8E_000237"
     CDS             complement(103181..104068)
                     /locus_tag="ACHK8E_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0342564.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(104093..104971)
                     /gene="yihU"
                     /locus_tag="ACHK8E_000238"
     CDS             complement(104093..104971)
                     /gene="yihU"
                     /locus_tag="ACHK8E_000238"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP0342565.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            105144..106040
                     /locus_tag="ACHK8E_000239"
     CDS             105144..106040
                     /locus_tag="ACHK8E_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0342566.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            106080..106868
                     /locus_tag="ACHK8E_000240"
     CDS             106080..106868
                     /locus_tag="ACHK8E_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0342567.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(106928..108193)
                     /gene="yihN"
                     /locus_tag="ACHK8E_000241"
     CDS             complement(106928..108193)
                     /gene="yihN"
                     /locus_tag="ACHK8E_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0342568.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(108295..109275)
                     /gene="yihM"
                     /locus_tag="ACHK8E_000242"
     CDS             complement(108295..109275)
                     /gene="yihM"
                     /locus_tag="ACHK8E_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP0342569.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(109283..109993)
                     /gene="yihL"
                     /locus_tag="ACHK8E_000243"
     CDS             complement(109283..109993)
                     /gene="yihL"
                     /locus_tag="ACHK8E_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0342570.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(110210..112033)
                     /gene="typA"
                     /locus_tag="ACHK8E_000244"
     CDS             complement(110210..112033)
                     /gene="typA"
                     /locus_tag="ACHK8E_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP0342571.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            112405..113814
                     /gene="glnA"
                     /locus_tag="ACHK8E_000245"
     CDS             112405..113814
                     /gene="glnA"
                     /locus_tag="ACHK8E_000245"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP0342572.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            113988..115037
                     /gene="glnL"
                     /locus_tag="ACHK8E_000246"
     CDS             113988..115037
                     /gene="glnL"
                     /locus_tag="ACHK8E_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP0342573.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            115049..116458
                     /gene="glnG"
                     /locus_tag="ACHK8E_000247"
     CDS             115049..116458
                     /gene="glnG"
                     /locus_tag="ACHK8E_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP0342574.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            116570..116680
                     /gene="yshB"
                     /locus_tag="ACHK8E_000248"
     CDS             116570..116680
                     /gene="yshB"
                     /locus_tag="ACHK8E_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP0342575.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(116870..118243)
                     /gene="hemN"
                     /locus_tag="ACHK8E_000249"
     CDS             complement(116870..118243)
                     /gene="hemN"
                     /locus_tag="ACHK8E_000249"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP0342576.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(118432..118941)
                     /gene="yihI"
                     /locus_tag="ACHK8E_000250"
     CDS             complement(118432..118941)
                     /gene="yihI"
                     /locus_tag="ACHK8E_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP0342577.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(119060..119226)
                     /locus_tag="ACHK8E_000251"
                     /pseudo
     CDS             complement(119060..119226)
                     /locus_tag="ACHK8E_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            119523..120155
                     /gene="yihA"
                     /locus_tag="ACHK8E_000252"
     CDS             119523..120155
                     /gene="yihA"
                     /locus_tag="ACHK8E_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP0342578.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(120479..123265)
                     /gene="polA"
                     /locus_tag="ACHK8E_000253"
     CDS             complement(120479..123265)
                     /gene="polA"
                     /locus_tag="ACHK8E_000253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP0342579.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT        30960 a        32394 c        30575 g        29528 t
ORIGIN      
        1 tcgagtgccc acacagattg tctgataaat tgttaaagag cagtgccgct tcactttttc
       61 tcagcggcgc ggggtgtgca taatacgcct tcccgctaca gagtcaagca tttttttgct
      121 tttctctgtt gagattctca ggagaacccc gctgacccgg cggcgtgttt gccgttgttc
      181 cgtgtcagtg gtggcgcatt atagggagtt attccggcct gacaagagga aatttaaaat
      241 aattttctga ccgcgcaaca ttcaaccaaa tcagcctaaa actgccagtt tttcgagcgt
      301 ttcgaagccg taacgctgta aaacgggcaa taattgttca gcttctggcg tcattgccat
      361 acaaaaggca atattcgcat cggcagattt tgcatccggg gcttcatgtt ctaacagcca
      421 ggccgttcgg cgagcaatcg ctgcgccaga atccaccagc cgggttccct ctggcagcac
      481 ctgtaacagt tcttcttgta gtagagggaa atgggtacac cccagtacaa cggtatctgg
      541 cggctctttc attcttaacc acggacgcaa gatacgtttt agcgcatcca gagaaacatc
      601 ttcgccatgc agcttcgctt cagccaactc gaccatctct gccgagccca gcatttctat
      661 ctggcattca ttagcgaaac gcgcgatcag ctcatgagta taagaacgtt taacagttcc
      721 gcgggttgcc agtaatccga caatgccatt tgccgtcaga cgtgcagcag gtttaatcgc
      781 cggcacgaca ccaacaaccg ggaagtcgaa cttttcgcgt aatgcaggaa gtgaaacggt
      841 actggcggtg ttgcaagcga ccacagccag cgcaagggga taacgttctt gcactgcggt
      901 gacaattgcc accactcgct caacaataaa ctcttcgctt ttttcgccat acgggaaagc
      961 gacgttatcg aaagcataaa tgtaatggag atccggtaag agatgccgga tctcgtcata
     1021 gaccgacaac ccaccgacgc cggagtcaaa caccagcacg gtgggacgtg gttcagaagg
     1081 tgtagctgcc agacaaggtg tattcccgtc ctgcagtttg gtagccatag actgtctcat
     1141 aatctttgtc gaacaggttg gctattttac cacgaactgt tagatgagag gtgaccggat
     1201 acgcaaccgc aagatcccac aagctcacac cgcctaattt aactgtatgt gatgagccag
     1261 aggtctcgtc ataagcgttg tcgtatcgtt gacctatata ttggtaagat acgtcccatt
     1321 cgaaaccata agcttgtcca gtcaactcgt acttaacctg ctgttttgcc cgacggtaca
     1381 gcactttatc tgtttcatca tcgcgcgggt caacatattg taacgtcaag cgatgttcaa
     1441 caggaccggt tgtaatattt cccgtccatt ccagcccttt aatcgtggct gatttcacat
     1501 taatgtactg gttgacatta tttatgcttt tataatcaat caggttctga atttcataac
     1561 gatatcctga gagccgccaa tccagagtcc cagttacccc ctcaattcca atctcccact
     1621 gtttagattc ttctggcttc aaatttgggt tggctgcaat accaggacca taaattgaag
     1681 caaagcgagc agccccgtat tgctgtccaa gagaaggcga aagaaaactg gtaccgtaag
     1741 aaagtgttgc ctggtagcca tcaataaact cccagccagc agctgtctgc caggtaccat
     1801 gccagccaaa ctgttcatca tgatcttctc ggccagaagc ttccaaagtc acattggaaa
     1861 tctgttgctg tccagtcagg tataacccgg tggtatcgcg ttcgtaatca tctgattttg
     1921 taatgctgct ggacgtcagt ttttcctgtt tccaatcgat cccgccgcta attgcaccat
     1981 gccctacctc aatgctatta ccccactgaa tataacgctg ttccatgtca tccagggtgg
     2041 catctcccgc ataacgacca agattatttg tgtagttata attcttgagt cgttgatagt
     2101 tagcaattaa ctgagacgaa tagatgccgg agttatagcg taggccagta tcccaggact
     2161 ggttgtaatt ctgagcttct tcgttgccag tagcagaacc atacatcgag ccctggtcgt
     2221 aatcattatt agctgtgtag ctgtaaccac ggaagaaccc cgagaagtta tcgttaaact
     2281 tatgctgcag ccccccccag aaaagtttgt tgcgatatcc atcgcgatcg ctatcaccac
     2341 tgtatggtga gccaggctga atgttaaaac ctttggtcgt ctggtaagca cctgctgctg
     2401 ttaccatcgt atcgccaaaa cgtttattta ttaccccatc ataggcttga taaccatttg
     2461 tacccattcc tgcgttgatc tgagctttct catcattact cattgtaata atgttcacca
     2521 cacctccgat ggcaccagaa ccataaacgg cagaacgcgg cccacgaata tattcaacac
     2581 gctgtacaag tgataccggg aactggctaa tatcaattgc attgtcgata ccggcacggg
     2641 ccatcggcac accgtcaatc aacactaaaa catggcgcga ttcagtgcct cgaacataaa
     2701 gagaagaact cgtccccatt ccaccagttt gagagatatt tacaccgggg agatgactca
     2761 taacatcgtt gaggtctttc gactgccagc gttgaatatc ctcacgggta acaatatcag
     2821 taggtgcaag gacagtattt actggctgct gaaaacggtt agcagtaaca acgagagtat
     2881 ccgggctggt atcctgtgcc caagcggaaa aggctgtgac agaacacgcc gtcagcagcg
     2941 aagctttttt aatcattgta aagcatccac aatagaagaa ggatgccgca ggtttcatca
     3001 atattacgcg atgatgagaa ccagatgcga cgttagccgg caggtcttcg ggcttggagg
     3061 ggtatctaag atactaagag atgatgactt cccaccggat ggcagtgtcc gcataacgca
     3121 atcatcgcac ctttccttac cgctgcgcgt cagctccaga ttcgcactgg attccctatt
     3181 aactcacagg accggcaagt ggatgctaca ggttgtaaca agttactgtc cagacgtagc
     3241 tcacaaatag gaattcatca agatctggac atctgatgag caatccctac aatcgccgcg
     3301 tactttaatt tttcaggata catcatgacc cccgaacacc ttccaacaga acagtatgaa
     3361 gcgcagttag ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt ttctgacctg
     3421 gttccggaag tgtttcgctc gccggtcagt cattaccgga tgcgtgcgga gttccgcatc
     3481 tggcacgatg gcgatgacct gtatcacatc attttcgatc aacaaaccaa aagccgcatc
     3541 cgcgtggata gcttccccgc cgccagtgaa cttatcaacc agttgatgac ggcgatgatt
     3601 gcgggtgtgc gtaataatcc cgttctgcgc cacaagttgt tccagattga ttacctcacc
     3661 acgctgagta atcaggcggt ggtttccctg ctgtaccata agaagctgga tgatgagtgg
     3721 cgtcaggaag cggaagccct gcgcgatgca ctgcgagcgc agaatctgaa tgtgcatctg
     3781 attggtcggg caacgaaaac caaaatcgag ctggaccagg attacatcga tgaacgtctg
     3841 ccggtcgcag ggaaagagat gatctaccgt caggtagaaa acagctttac ccagccgaac
     3901 gcggcgatga atattcagat gctggaatgg gcgctggacg taaccaaagg ctcaaaaggc
     3961 gatttgctgg agctgtactg cggcaacggt aacttttcat tagcactggc gcgcaatttt
     4021 gatcgggtat tagccaccga aatcgctaag ccgtcggtcg ctgccgcgca atacaacatt
     4081 gccgctaacc atattgataa cgtacaaatt attcgtatgg cggcagaaga atttactcag
     4141 gcgatgaatg gcgtacgcga gtttaaccgc ctgcaaggaa tcgacttaaa gagttatcag
     4201 tgcgaaacca tttttgtcga cccaccgcgc agcggtctgg acagtgaaac cgagaaaatg
     4261 gtgcaggcgt atccacgtat tctgtatatc tcctgcaatc cggaaacgtt atgcaagaat
     4321 ctggaaacat taagccagac gcacaaggtc gaacgtctgg ctctgtttga tcagttcccc
     4381 tacacgcacc atatggagtg cggcgtatta ctgaccgcga agtaaaaccc atgccggatg
     4441 caccagcatc cggcatgata ccgattactc agcaacttcc tgcttacggt tacgcatctt
     4501 cgcgccaatc cagaacacca taatgacgga gagtactgcc gggaagaagt tagagccgat
     4561 atccggatat tccgcacgaa ctaccgtgct gtacagcagt acgccgagaa taaaacaggc
     4621 agctgccaga cccggcaagc ctaccggcat ggtgcgatta agataacgtt gatgcaggca
     4681 gtaaaccgtc agcaacaggg aaataatcgg gaatacagaa aatggcacaa tggagctaaa
     4741 cagcgctgcg aaagtaccat taatcgataa gccagcaacc aacgccagca gcagcgtacc
     4801 tctatcttga tttgcttgtt tcattactcg tccttcacat ttcccggaat aattgcggtt
     4861 ttctcttgtt cacggcgata ccagtaataa gcccctttag aaatcattcg cagttgcagt
     4921 accagtcgct cttctaattg ccgacgttgt tcgacgccga catccaacgc ctcggccccc
     4981 gcactgaaga caatcgtcac cattgcttcg gcttgcgctt cagtaaacgc acgcggcata
     5041 tggttttcga gttccagata gtccgcaagt tccgcaatga agtgctgaat ttcacgcgca
     5101 acggcggcac gaaacgcagc ggaggtgccg gagcgttccc gcaataataa ccggaaggcg
     5161 ttaggattat taccgatgaa ctccataaat gtggagaccg aggtgcggat cacactcccg
     5221 cctttggcga tacgctgacg cgcctgacgc atgagttggc gtagcattaa accgctctca
     5281 tcaaccatgg tcagacccag ttcgtctacg tcgcggaaat gccgataaaa agaggtggga
     5341 gcaatgcccg cttcacgcgc cacttcacgc aaactcaggc tggcgaagct gcgttcagca
     5401 cttaattggc taaatgcggc ttccaccagc gaacggcggg ttttttcttt ttgttgcgct
     5461 cttacgccca tcacgatgtc tgaatccttg ccaaaagtac tgctggcact ataccagaga
     5521 atgaacatac tggattgctc cagtattgtg aatgaacggt aacgcaataa aacatattta
     5581 ctgcaattct ttggccggtt cttttacgta caacggaaac ctgccgctta aacggagagt
     5641 atcgtcgata aaaatccaat aaaacgttca ggtcaaaagt aagaaacaga caaagcaaag
     5701 gccgctcacg atatagccag ataaatgacg gggatcaatt ggcttacccg cgataaaatg
     5761 ttaccattct gttgctttta tgtataagaa caggtaagcc ctaccatgcc acattcctac
     5821 gattacgatg ccatagtaat aggttccggc cccggcggcg aaggcgctgc aatgggcctg
     5881 gttaagcaag gtgcgcgcgt cgcagttatc gagcgttatc aaaatgttgg cggcggttgc
     5941 acccactggg gcaccatccc gtcgaaagct ctccgtcacg ccgtcagccg cattatagaa
     6001 tttaatcaaa acccacttta cagcgaccat tcgcgactgc tccgctcttc ttttgccgat
     6061 atccttaacc atgccgataa cgtgattaat caacaaacgc gcatgcgtca gggattttac
     6121 gaacgtaatc actgtgaaat attgcagggg aacgctcgct ttgttgacga gcacacgctg
     6181 gcgctggatt gcccggacgg cagcgtcgag acactaaccg ctgaaaaatt tgttattgct
     6241 tgcggctctc gtccatatca tccaacagat gttgatttca cgcatccacg catttacgac
     6301 agcgactcaa ttcttagcat gcaccacgaa ccgcgccatg tacttatcta tggtgctgga
     6361 gtgatcggct gtgaatatgc gtcgatcttc cgcggtatgg atgtaaaagt ggatctgatc
     6421 aacacccgcg atcgcctgct ggcatttctc gatcaagaga tgtcagattc tctctcctat
     6481 cacttctgga acagtggcgt agtgattcgt cacaacgaag agtacgagaa gatcgaaggc
     6541 tgtgacgacg gtgtgatcat gcatttgaag tcgggtaaaa aactgaaagc tgactgcctg
     6601 ctctatgcca acggtcgcac cggtaatacc gattcgctgg cgttacagaa cattgggctg
     6661 gaaactgaca gccgcggaca gctgaaggtc aacagcatgt atcagaccgc acagccgcac
     6721 gtttacgcgg tgggcgacgt gattggttat ccgagcctgg cgtcggcggc ctatgaccag
     6781 gggcgcattg ccgcgcaggc gctggtgaaa ggtgaagcca ccgcacatct gattgaagat
     6841 atccctaccg gtatttacac catcccggaa atcagctctg tgggcaaaac cgaacagcag
     6901 ctgaccgcga tgaaagtgcc atatgaagtg ggccgcgccc agtttaaaca tctggcacgt
     6961 gcacaaatcg tcggcatgaa cgtgggcacg ctgaaaattt tgttccatcg ggaaacaaaa
     7021 gagattctgg gtattcactg ctttggcgag cgcgctgccg aaattattca tatcggtcag
     7081 gcgattatgg aacagaaagg tggcggcaac actattgagt acttcgtcaa caccaccttt
     7141 aactacccga cgatggcgga agcctatcgg gtagctgcgc taaatggctt aaaccgcctg
     7201 ttttaacact ttatcgaaat ggccatccat tcttgcgcgg atggcctctg ccagctgctc
     7261 atagcggctg cgcagtggtg agccaggacg ataaaccagg ccaatagtgc ggcgtggttc
     7321 cggcttaatg catggcagat aaacaacccc atcacgtttg cgttccggcg gcacagccag
     7381 cgcaggcagt aaagtgatcc cgctacctgc cgccaccatg ttgcgcagag tttccaggct
     7441 ggtcgcgcgg aagtgtgtat cttcatccgc cccggcttca aaacagaaac ccattgcctg
     7501 atcgcgcaaa cagtgaccat cttccagcat cagcagtttt tcccctgcca gatcggccat
     7561 cggtacgcat tcgcggttcg cccacggatg gtcttcatag atagccagca acattggctc
     7621 atcaaacaac ggcacttcaa tgaatgcttc gctctctttc accagcgcga ggatcacgca
     7681 atcgagtttg ccgctgtcca gttgcgccag taactggtgg gtctgtgctt catgcagata
     7741 catttccagc tttggaaagg tctggtgcag catcgggata atatgcggta gcaggtacgg
     7801 tccaaccgtg ggaatcagac caatatgcag cggtccggac atcgtctcgc cctgctggct
     7861 tgccatctct ttaaggactt tcacctcacg cagcacggta cgcgcctgat ccaccagcag
     7921 cattcccgcc tgggtgaata acactttacg gctggtccgc tccagcaaca tcacacccaa
     7981 ctcatcttcc agcttacgaa tttgcccgct aagcgtcggc tggctaacgt ggcaggaatc
     8041 tgccgcacgc cgaaaatggc ggtgttcggc caatgccacc aggtactcaa gatcacgaat
     8101 attcattatc catcctccat tgccacgata gttcgcggcg ataggtagca tagcaacgaa
     8161 cgattatccc tatcaagcat tctgactgat aattgctcac agaaacggag cggcacctct
     8221 tttaaccctt gaagtcactg cccgtttcga gagtttctca actcgaataa ctaaagccaa
     8281 cgtgaacttt tgcggatctc caggatccgc ttttttttat tctacaggcc cacagcaaga
     8341 gagcctgata gtaacggtta ctcaatccga tctttgacaa taaagaagta acacagcgcc
     8401 ccggtgaacg tcacacaggc cgcgataacc agcgccagat taaacgagtg tgtggtatcc
     8461 acgacccaac cggttacgat cggagcaaag gaagcaaaaa caaaactgcc aaagttttga
     8521 atcccggcga tggaagccac ttttgtttct gacaccatca cctgcaccag cccccatgca
     8581 gatgttcccg cgaaatgcac gcagaacagc gccattgaga taaacgctac cgcctgggct
     8641 ggcgaggaag attgcacgac cagcaacgtg cctaatgccg acatcatcaa accgcagaca
     8701 atcgccgttt tacgcgtctt cgccaggtca tagccttttt tcgccagccg atcgacaaca
     8761 ataccgttaa cccacatccc gaccgccgcc gcgaggaaag gaattgccgc cacccaaccg
     8821 gttttcgcca gactgaaacc ttgttcggcc tgcaaataac cgggtaacca cgcgatgtag
     8881 agccaaccgg tataattgac gccagagaaa cctaaaatca tcccccaggt tgtccgatgc
     8941 ttaaacagcg ccagccactc gctaaattgt aactgtggac gcggtttaac cggcgcggag
     9001 agatatgtcc gttcttcctc ggtcaggaca aactgcgccc ggttgcgata ccacgcgtac
     9061 caacagatcc caaccagaat ccctgctacg ccgatgatga caaacatcgt ccgccagccc
     9121 catgccagtt gcatcaatac aagagcagga ggcgcgatag cctgaccgat aacggtagat
     9181 gagttaaaaa tccccagcgc cgtgccgcgt tctttttgcg cataccagtc ggtgatcgac
     9241 tttactcccg caggcataaa tggcgcttcg ccaataccca gaccgatacg cattaaaata
     9301 aagtggctaa aagaattaac cattcctgtt aatgcctgca ttaatgacca gaatattaag
     9361 cctgcgccta acactattcg tgggccaaag cggtctagca aaataccgga aggaagttgt
     9421 gaaaaaccat aagacagaga aaatgcagaa agcaaaacac caaactcggt agcggataat
     9481 cctaactcgc cacgaattgc ttcacctgcc acgctcaatg acgaacggtc gagaaaatta
     9541 acgatccccg ccataaataa taaaaccagg gtcactgtct gaatacgacg aatccgttgc
     9601 ggtttattac ccgcaacact ggttgacaca tcaacgtcca tttttagctc cttgagcgga
     9661 attaatcgat ttgatgaata ttcagaaaat aattaagcga accgctatta ccagttccac
     9721 aatgtgccat cttccagtcg tgcgacgggg agatacgccg ggtcatacgg atatttcgcc
     9781 gccagtttct catcgaaaga aatacccagc cccggttcat cgcctgggtg catataacct
     9841 tcatcgaagc gccagctatg cgggaagact tcaagcattt gttcggaata gcccatgtac
     9901 tcctgaacgc caaagtttgg cacccagagg tcaaaatgca gcgccgccgc atggcaaaca
     9961 ggcgataaat ctgaagggcc atgcgaaccg gtacgtacct gataaagcga tgcaaaatcg
    10021 gcaatccgtc gcatcccggt aatgccgcct gcatgggtaa tcgtcgtgcg aatatagtca
    10081 atcagctgct cttcgattaa ctgcttgcag tcccagatac tgttaaacac ttcaccgacc
    10141 gcaatcggcg tcacggtatg ctgacgaatc agacgaaaac attcctgatt ctcggcaggc
    10201 gtcggatctt ccatccagaa caggcggtaa tcttcaatgc tcttcccaaa gcgcgccgct
    10261 tcgatggggg tcaggcgatg gtgcatgtca tgcaacagat gttcgttaaa accgaactta
    10321 ttacgcaccg cttcaaataa cttcggcgta aaatcgagat acttttctga agaccacaac
    10381 tgctcttctg gccacaggcc tttggttgct ggttcatacg ccagtccttt gcccttcgcc
    10441 agaccatagg tggtttgcat acccggtaca ccacactgca cacgaatcgc cttaaagccc
    10501 atctgctggt gctttgcata gtcttcaagg acttcatcaa tcgtacgccc ggtggtgtgg
    10561 caataaacca taacgccttc acgagaagca ccaccaagca gttgatacaa tggcatattt
    10621 gccgcttttg ctttgatatc ccacaacgcc atatcaatgg cagaaatggc tgacatcgtg
    10681 acggggccac gtcgccagta agcaccttta taaaagaatt gccagatatc ttcgatacgg
    10741 ctggcatcgc ggccaattag ttgtggacaa agatgatctt tcagataaga agcgaccgac
    10801 aattcgcgac catttaacgt ggcatctcct aatccacaaa gtccgcttgc agtggttatt
    10861 ttaagcgtaa cgaaattacg ccctgggcaa gtaacaaaaa catctgcagc aataattttc
    10921 attattgcaa tctcctgtgt tgtaaataga cgtttcaaac ttaattttgc gcctactacc
    10981 atacaagaga gaaagagcga ttattcaacc atgatcacat taaaaataaa aatttctacc
    11041 gaggagaaaa aacaccctca aaaaccacct gaataattag ctatcatcgt taaaacaaca
    11101 cgttaaataa tcacataaca aactccatta atcattaaaa atgaacacag caatagaaat
    11161 gaattatatt caattaatta aagtatgtat tggctgatgt attgaaaata taatttttat
    11221 ggggagagtg aaggtaaaga agacgcaaga ataaaaagga atgtgacaaa taatggttct
    11281 ggttgggcgt taaatttaag gtcaccagaa tactgacaaa tttctgcctg atgcggcgta
    11341 aacgccttat ccgcctacga agtgtcgcac tttgtaggcg tggtaagctc cccattttca
    11401 ggggagcaat ataaacaaac tactctaacc gagcttgcgc aaaagcaatc gcctgcgcca
    11461 cctgctgcgg tgagacgccg ccttttgcag cacgcttgtc gaggcacgat tgcagcgaca
    11521 gaatcggata gacatcttcg ccaatcacct ggctgaattt ctgcaactca ctgagcggca
    11581 gatcttccaa cgctttgccc tgacgaatgg cttccaccac agcttcaccc acaatatggt
    11641 gcgcctcgcg gaacggtacg cctttcgcca ccagataatc cgccagttca gtagcgttgg
    11701 cgtaaccctg ctgcgccgct tcctggcaac gcggacgttt cacctgaatg ccgtccagca
    11761 ccagcgccgc catatgcagg cagtccagcc aggtatcgag cgcgtcgaac agaccttctt
    11821 tgtcttcctg catatctttg ttgtaagcca gcggcaaacc tttcagcgtc atcatcatgc
    11881 cggttaacgc cccctgcacc cgaccgcatt taccgcgaat cagctccagc gcatccgggt
    11941 ttttcttctg cggcattaat gatgaaccgg aagtcacacg gtcagaaagc tccacaaacc
    12001 ccgcttcgcc ggtgttaaag aaaatcagat cttcagcaaa acgcgacaga tgcaccatgc
    12061 cgatagcggc agcagaaagc agttccagca catggtcacg gtcagaaacg ctgtcgagac
    12121 tgttacgggt cgccgaagca aagcccagcc agcctgctaa ctgttcacgg tcgatttcat
    12181 aggccgttcc tgccagtgcg ccacagccta gcgggctgac atccagacgc ttaagcgcgt
    12241 cctgcaaacg gctttcatca cgcgccagca tctcaacgta ggccaggcac cagtgagcga
    12301 acgtcaccgg ctgggcgcgt tgcaggtgag tgtaacccgg catcaccgcg tcctgattgt
    12361 tctgcgcggt ctcgaccaga gcggattgta actgccggtt agccgtcagc aactcgctaa
    12421 cggtatcttt gcaccacagt ttcaggtcag ttgctacctg atcattacgg ctacgcccgg
    12481 tatgcagttt tttgcctaac tgacccactt tgtcgatcag tttgctttcc acccagctat
    12541 ggatatcttc ggcgtcgctt tcaaggattt gttgtggcct ggcgcgaaca tcttccagca
    12601 gcacgttcag cgcctcttcc agttgcgcct gctcttctgc ggttaacacg ccgaccgtta
    12661 ccagggcttt ggaccaggcc acagagccaa caatatcctg ctccgccaga cggtaatcaa
    12721 agcgcagtga gtcgttgaat tgtttgaacc gttgatctgc tgcctgggta aaacgcccgc
    12781 cccaaagtgc cataactctg tttccttatt ttgaaattca atgccggaaa gctgcgcctc
    12841 cggccaacaa aacttaagct aaaatccgcg tacccatcgg cataccgtta aacagtgccg
    12901 gaagctgttc cgcatgacgc caggaggcga tatctaccgg acggcccaac gtgcgcgcag
    12961 catccagcgc cgcgttcact ttcactatca tgccgtcagt aataataccc tgctcaatca
    13021 gttgttctgc tttcgcggcg gtcatttcgg caatacgttg ccctttgccg tcgagaatgc
    13081 cactgacgtc ggagagcaaa atcagatccg cgcccagcgt tgccgccagc gccgttgccg
    13141 cctgatcggc attgacgttc atcagttgcc cttcgtctgt tacgccaatg gagctgacca
    13201 ccggcagata accgttctcc agcaatgagt tgataagctt aggcgaacct ggctgcgcca
    13261 gtccaacatg gcctaactct tcatcaagct gggtcacttt gacgctgtcg ccgtcaccga
    13321 gaaacaaacc tacggccgca atctgatgtt tcttcgccca tgccagcagg gttttatttg
    13381 ccgttcccgc cagtgctccg gtgataatgt caatctgatc agcaggcgtc acccgcaggc
    13441 catttttctt tttcaccggc agattcagcc ctttcatcaa ctcatccact acgcagccgc
    13501 caccgtggac aatcaccagc ggacgctgat gtgactcacg ataattcacc agtgcactaa
    13561 acagacgttc cagcgcctcc tcactatcca gcagtacgcc gcccagtttg ataattaatg
    13621 gattcatcat tgcaccctta aataagagac tgcgtttccg catagccgaa acgaatattg
    13681 gcgcactgca ccgcttgtgc cgccgcgcct ttcagtaagt tgtcttcggt cgccacaata
    13741 atcagatgct cgccctgaac ggcaaaaccg atatcgcaga atggcagtcc aacgacattt
    13801 ttcagcgccg gaacgccttt gtcatacagc cgcaccagtg gtttatgggc atacgcctgc
    13861 tgtaacgctt gcgccacttg cacctggctc acacccggtt tcaggcggca ggtaatggtt
    13921 tcgagaatgc cgcgcgggaa attgcccaga tgcggggtga agataacgtc agcaccgagg
    13981 tgtgtggcga tctctggttg atggcgatgg gtaaagacgc catacggttg caggctaact
    14041 tcacaaaaac tatttgaaat cgctgcctta cgtcctgcac cgctcacgcc gctggtggcg
    14101 ttgatcaccg gccactgatt gaggtcaaga agatcggcat caatcaacgg tttcagcgcc
    14161 agctgtgctg ccgtcggata acagcctggc acggcaatca agttcgcttc ttttaattta
    14221 ttaccgcacc actccgccag accgtaggct gcctgttcca gcagttccgg gtattgatgg
    14281 gtaaagccgt aatatttttc atagaaggcg acatcgttaa cacgaaacgc gccggaaagg
    14341 tcgaacacca cacagcctgc ttcaagaaat tgcggcgcta aatcgtggct gacttcatgg
    14401 gcggtggcga gaaacaccac gtccacccct gggctaaact cgctgatatc cgacatcggc
    14461 tgcaacggca gatcaacgat gccttttagc tgcggatgca aatcggagat taactttccc
    14521 gcatcattgc tttgcgctga aacagtcaaa gcggttatgt tcatatgcgg atggcgattt
    14581 acataggtca ctagctctgc gccagcgtag ccgctggcac ccacaatcag cgtattcaac
    14641 atcggggcta ttcaccttct tatgtctggt tgccaggtta aacgtaaaac attcacctta
    14701 cggctggtgg gttttattac gctcaacgtt agtgtatttt tattcataaa tactgcatga
    14761 atattgatac tatcatgacc agaggtgtgt caacaatgaa aaacaaatta ccgccattta
    14821 tcgagattta ccgtgctctg attgccacac cttcaataag cgccacggaa gaggcactcg
    14881 atcaaagcaa tgcagattta atcactctgc tggcggactg gtttaaggat ttaggcttca
    14941 atgtggaagt acagcctgtg ccaggaactc gcaacaaatt taatatgctg gccagttgcg
    15001 gacagggggc tggcggtttg ttgctggcgg ggcataccga tacggtgcca tttgatgacg
    15061 gacgctggac gcgcgatcca tttacgttga cggagcatga cggcaagctt tacggcttag
    15121 gcaccgccga catgaaaggc ttttttgcgt ttatccttga tgcgctacgc gatgtcgacg
    15181 tcacgaagct tgcaaaaccg ctctacattc tggcgactgc cgatgaagaa accagtatgg
    15241 ccggagcgcg ttattttgcc gaaactaccg ccctgcgccc ggattgcgcc atcattggcg
    15301 agccaacgtc gctgcaaccg gtacgcgcgc ataaaggtca tatctctaac gccatccgta
    15361 ttcagggcca gtcgggccac tccagcgatc cagcacgcgg agttaacgct atcgaactga
    15421 tgcacgacgc catcgggcat attttgcaat tgcgcgataa cctgaaagaa cgttatcact
    15481 acgacgcgtt taccgtgcca taccctacgc tcaacctcgg gcatatccac ggtggcgacg
    15541 cttctaaccg tatttgtgct tgctgtgagt tgcatatgga tattcgtccg ctgccaggca
    15601 tgacactcaa tgaacttaat ggtttgctca acgatgcatt ggctccagtg agcgaacgct
    15661 ggccgggtcg tctgacggtc gacgagctgc atccgccgat ccctggctat gaatgcccac
    15721 cgaatcataa actggttgaa gtggttgaga aattgctcgg agcaaagacc gaagtggtga
    15781 attactgtac cgaagcgccg tttattcaaa cgttatgccc gacgctggtt ctggggcctg
    15841 gctcaattaa tcaggctcat cagcctgatg aatatctgga gacgaggttt atcaagccca
    15901 cccgcgaact gatcacccag gtgattcacc atttttgctg gcattaaaac gtaggccaaa
    15961 caatgtcgga tgcgatgctt acgcatctta tccgacctac acctttggtg ttactcgagg
    16021 cgatttttta acattcccat aagttacgct tatttaaagc gtcatgaatt taatggcgta
    16081 aattcctgct atttattcgt ttgctgaagc gatttcgcag catttgacgt caccgctttt
    16141 acgtggcttt ataaaagacg acgaaaagca aagcccgagc atattcgcgc caatgcgacg
    16201 tgaaggatac agggctatca aacgataaga tggggtgtct ggggtaatat gaacgaacaa
    16261 tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag tgctgggaga aaccatcaag
    16321 gatgcgttgg gagaacacat tcttgaacgc gtagaaacta tccgtaagtt gtcgaaatct
    16381 tcacgcgctg gcaatgatgc taaccgccag gagttgctca ccaccttaca aaatttgtcg
    16441 aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt tcctgaacct ggccaacacc
    16501 gctgagcaat accacagcat ttcgccgaaa ggcgaagctg ccagcaaccc ggaagtgatc
    16561 gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga gcgaagacac catcaaaaaa
    16621 gcagtggaat cgctgtcgct ggaactggtc ctcacggctc acccaaccga aattacccgt
    16681 cgtacactga tccacaaaat ggtggaagtg aacgcctgct taaaacagct cgataacaaa
    16741 gatatcgccg actacgaaca taaccagctg atgcgccgcc tgcgccagtt gatcgcccag
    16801 tcatggcata cagatgaaat ccgtaagctg cgtccaagcc cggtagatga agccaaatgg
    16861 ggctttgccg tagtagaaaa cagcctgtgg caaggcgtac cgaattacct gcgcgaactg
    16921 aacgaacaac tggaagagaa tctcggctac aaactgcccg tcgaatttgt tccggtccgt
    16981 tttacctcgt ggatgggcgg tgaccgcgac ggcaacccga acgtcactgc cgatatcacc
    17041 cgccacgtcc tgctgctcag tcgctggaaa gccaccgatc tgttcctgaa agatattcag
    17101 gtgctggttt ctgaactgtc gatggttgaa gcgacccctg aactgctggc gctggttggc
    17161 gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa acctgcgttc tcgcctgatg
    17221 gcgacacagg catggctgga agcgcgcctg aaaggcgaag aactgccaaa accagaaggc
    17281 ctgctgacac aaaacgaaga actgtgggaa ccgctctacg cttgctacca gtcacttcag
    17341 gcgtgtggca tgggtattat cgccaacggc gatctgctcg acaccctgcg ccgcgtgaaa
    17401 tgtttcggcg taccgctggt ccgtattgat atccgtcagg agagcacccg ccataccgaa
    17461 gcgctgggcg agctgacccg ctacctcggt atcggcgact acgagagctg gtcagaagcc
    17521 gacaaacagg cgttcctgat ccgcgaactg aactccaaac gtccgcttct accgcgcaac
    17581 tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct gccaggtgat tgccgaagca
    17641 ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga aaacgccgtc cgacgtgctg
    17701 gctgtccacc tgctgctgaa agaagcgggt atcgggtttg cgatgccggt tgctccgctg
    17761 tttgaaaccc tcgatgacct gaacaacgcc aacgatgtca tgacccagct gctgaatatc
    17821 gactggtatc gcggcctgat tcagggcaaa cagatggtga tgattggcta ttccgactca
    17881 gcaaaagatg cgggcgtgat ggcagcttcc tgggcgcaat atcaggcaca ggatgcatta
    17941 atcaaaacct gcgaaaaagc gggtattgag ctaacgttgt tccacggtcg cggcggttcc
    18001 attggtcgcg gcggcgcacc tgcccatgcg gcgctgctgt cacaaccacc aggaagcctg
    18061 aaaggcggcc tgcgcgtgac cgaacagggc gagatgatcc gcttcaagta cggtctgcct
    18121 gaaatcaccg ttagcagcct ttcgctgtat accggggcga ttctggaagc caacctgctg
    18181 ccaccgccag agccgaaaga gagctggcgt cgcattatgg atgagctttc ggttatctca
    18241 tgcgatgtct accgcggcta cgtgcgtgaa aacaaagatt ttgtgcctta cttccgctct
    18301 gccacgccgg agcaggaact cggcaaacta ccgctgggtt cacgtccggc aaaacgtcgc
    18361 ccgaccggtg gcgttgagtc actgcgcgct attccgtgga tttttgcctg gacacaaaac
    18421 cgcctgatgc tccccgcctg gctgggtgca ggtacggcgc tgcaaaaagt ggtcgaagat
    18481 ggcaaacaga gcgagctgga agccatgtgc cgcgattggc cattcttctc gacgcgtctc
    18541 ggcatgctgg agatggtctt cgccaaagca gacctgtggc tggcggaata ctatgaccaa
    18601 cgcctggtag acaaagcact gtggccgtta ggtaaagagt tacgcaatct gcaagaagaa
    18661 gacatcaaag tggtgctggc gattgccaac gattctcacc tgatggccga tctgccgtgg
    18721 attgcagagt ctattcagct acggaatatt tacaccgacc cgctgaacgt attgcaggcc
    18781 gagttgctgc accgctcccg ccaggcagaa aaagaaggcc atgagccgga tcctcgcgtc
    18841 gagcaggcgt taatggtcac tattgccggg attgcggcag gtatgcgtaa taccggctaa
    18901 tcttcctctc ctgcaaaccc tcgtgctttt gcgcgagggt tttctgaaat acttctgttc
    18961 taacaccctc gttttcaata tatttctgtc tgcattttat tcaaaatctg gatatccctt
    19021 cagatatcct taaggaattg tcgttacatt cggcgatatt tttttcaaga caggttctta
    19081 ctatgcattc cacagaagtc caggctaaac ccctttttag ctggaaggcc ctgggttggg
    19141 cactgctcta cttttggttt ttctctactc tgctacaggc cattatttac atcagtggtt
    19201 atagtggcac taacggcatt cgcgactcgc tgttattcag ttcgctgtgg ttgatcccgg
    19261 tattcctctt tccgaagcgg attaaaatta ttgccgcagt gatcggcgtg gtgctatggg
    19321 cggcctctct ggcagcgttg tgctactacg tcatctacgg tcaggagttc tcgcagagcg
    19381 ttctgtttgt gatgtttgaa accaacacca acgaagccag cgagtattta agccagtatt
    19441 tcagcctgaa gattgtgctt atcgcgctgg cctatacggc ggtggcagtt ttgctgtgga
    19501 cgcgcctgcg cccggtctat attccaaagc cgtggcgtta tgttgtctct tttgccctac
    19561 tttatggctt gattctgcat ccgatcgcca tgaatacgtt tatcaaaaac aagccatttg
    19621 agaaaacgtt ggataacctg gcatcgcgta tggagcctgc cgcaccgtgg caattcctga
    19681 ccggctatta tcagtatcgt cagcaactaa actcgctaac aaagctactg aatgaaaata
    19741 atgccttgcc gccgctggcg aatttcaaag atgaatcggg taacgaaccg cgcaccttag
    19801 tgctggtaat tggcgaatcg acccagcgtg gacgcatgag tctgtacggt tatccgcgtg
    19861 aaaccacgcc ggagctggat gcgctgcata aaaccgatcc gaatctgacc gtgtttaata
    19921 acgtggttac gtctcgtccg tacaccattg aaatcctgca acaggcgctg acctttgcca
    19981 atgaaaagaa cccggatctg tatctgacgc agccgtcgct gatgaacatg atgaaacagg
    20041 cgggttataa aaccttctgg atcaccaacc agcagacgat gaccgcccgc aataccatgc
    20101 tgacggtatt ttcgcgccag accgataagc agtactacat gaaccagcaa cgtacgcaga
    20161 gtgcgcgtga atacgacacc aacgtgctga agccgttcca ggaagtgctg aaggaccctg
    20221 cgccgaagaa actgatcatc gttcatctgc tgggtacgca tatcaagtac aagtaccgtt
    20281 atccggaaga tcagggcaaa tttgacggta atacagagca tgttccaccg ggattaaacg
    20341 cagaagagct ggagtcatat aatgattatg acaatgctaa cctgtacaac gatcacgtgg
    20401 ttgccagcct gattaaagac tttaaagcga ccgatccgaa cggtttcctg gtttatttct
    20461 ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa aactcagggg cgtaatgaag
    20521 acaacccgac gcgtcatatg tacaccattc cgttcctgct gtggacgtca gaaaaatggc
    20581 aagcgactca tccgcgtgat ttctcacagg atgttgatcg taaatacagc ctggcggaac
    20641 tgatccacac ctggtcagat ttggcgggct tatcttacga cggttacgat ccaacccgtt
    20701 cagtggtgaa tccgcagttc aaagaaacta cccgctggat tggtaacccg tataagaaaa
    20761 acgcactgat cgattacgac actctgccgt atggcgatca ggtgggtaat cagtaattat
    20821 cagccagatg ttatttgccg gatgcgacgc ttgacgcgtc ttatccggcc tacagatcgc
    20881 ggcggtaggc cggataaggc gtttacgccg catccggcaa tacaagtcga ctgaaactgc
    20941 cgcaatgttt acatctcact cccatcaggc cacacttttc aggtggcctt ttttcataat
    21001 gccagttact gttttcccgt ctacatacct aagggaataa catgtatcac gacgtcagct
    21061 atctgctctc ccgcctgatc aacggcccgc tgtccctgcg ccagatttac tttgccagca
    21121 gcaacggccc ggtgcccgat cttgcgtatc aggtcgattt tccacggctg gaaattgtgc
    21181 tggaaggtga gtttattgat accggcgctg gagcagcgtt agttcccggc gatgtgctgt
    21241 acgttcccgc tggtggctgg aattttccac aatggcaagc ccccgccact acctttagcg
    21301 tgctgtttgg caaacagcaa ctcggcttca gcgtcgtgca atgggatggc aaacaatatc
    21361 aaaatctggc gaagcaacac gtcgcccgac gcggcccacg cataggttct tttctgctac
    21421 aaacgctcaa tgaaatgcaa atgcagtcgc aggagcagca aacggcaagg cttatcgtcg
    21481 ccagcctgct tagccactgc cgcgatttgc ttggcagcca aatccagacc gcctcacgca
    21541 gccaggcact gttcgaagct attcgtgatt atatcgacga acgctatgcc tccgcgctta
    21601 cccgcgaatc tgtcgcacag gcattctata tttcgccaaa ttacctctcg cacctgtttc
    21661 aaaaaacggg ggccactggt tttaacgaat acctgaatca cacgcgactg gagcacgcta
    21721 agacgttatt gaaaggctat gacctgaagg taaaagaagt ggcgcacgcc tgcggctttg
    21781 tcgacagcaa ctacttctgc cgtctgtttc gtaaaaatac cgaacgctca ccgtcggaat
    21841 atcgtcggca gtaccacagc caactgacag aaaaaccgac tactccagaa tgagatgggt
    21901 ttgctgcggc gcagaaagca ctttgcgtac cgcgctcatc acccgctgcg gctcacgaag
    21961 gaaagcgtag atgctgcatt gcacatagcg gcaatggtca aatcgctcag caccggcaag
    22021 ctcgatatcc gtaattaaca gcgcaacatc cgcccggcgg atatcctcgt ccgctaaacg
    22081 attctccgtt cccagcgcgc cctgagtttc aatgctgacg ccccacttct ctaacaggca
    22141 caacttttcc agccgttccg ccgccatata agtatgcgcc acgccactga cgcaggcggt
    22201 tactgccacc aggtatgcca ttttaacctc ccacggtaac ctgaaatccg gcccgttctg
    22261 ccatttcgcg catcgttgcc acatcagctg acgacggcgc aggcacctct ttcatcgacc
    22321 atgttttccc cagcaggcgg tatttcggtt cgccgtactg atgaaacggt aacagatgga
    22381 tctgcctgat attcagcggg atcagcacgt ccagagcctg ctgcatattc tcctggctga
    22441 gcgtgaaacc agggatcagc ggtaaacgcg ggatcacgtt gacgccctca ctcaccagca
    22501 aacgcagatt ctccagcacg cgtggcagat tcatcttcac cacatcccgc gcctgagtcg
    22561 cgtccataat ttttaaatcg aacaacactt catcgcacaa tttcgccagc ggtaacagct
    22621 tgctggctgg cgcgtctccg gcagtttcaa tggcgcatga cacgccccac aaccgcagcc
    22681 gctgtaaaaa acgggtagca aactccgcct gcattaacac ttcgccgcca gaaagcgtga
    22741 cgccgccgcc ggacgtgcga aagaaaatgt catctttcat cacttcccgc tccagcgcgt
    22801 caaggctgat atcgcggcca atccgttcaa acgccccgga ggggcattca tccgcatcac
    22861 gcaaacattt cgcacagtgc agacatttcg cctctctgcg taccgtctgg attttgccgg
    22921 agatcgactc cggattagca caccatgggc aaagatgcgg acagccttta aaaaagacca
    22981 ccgtacgaat gccctcgccg tcattcagtg aataacgctg aatgttgaaa atgcgcgcca
    23041 catcattgcg gcgcgtttcc acaacgttac agctgatgcg ctgtccggcg gatgatgtca
    23101 tcctggatct ccttcgacaa ttcgacaaag aaggcgctgt atccggcaac gcgcaccacc
    23161 agcccggcat aatcttgtgg gcgctgttgc gcttcccgca acgtgtcggc gttcaccacg
    23221 ttaaactgaa tatgctgcaa cttaagctgg gtaaacgccc gtaagaagtc ggccagtttg
    23281 cgtaatcccg cttcaccttc cagagtcgcc ggagtgaatt tcacgttcag caacgtaccg
    23341 ttagacagca gcgtattatc gagcttactg actgacttca gtaccgccgt tggcccttgt
    23401 gcgtcctggc ccagcatggg tgacaagccg ccgtctgcca gttgttcccc ggcaaaacga
    23461 ccgtctggcg tcgcgccaac caccgatccc aacgggacgt gagcagaaac ggtatacgat
    23521 cccggcgtga agtagccgct gcgcgggttc tggtattttt ccacttcttt gcagtagtgg
    23581 cgtaacagtt cggcgctgat gttatccacc tcgtcgatat cgttaccgta tttctcgaag
    23641 cggttaatta agcgagcacg gactttttcg ccttctggcg ttgcgaagtt ggcttttaat
    23701 accgacagca attcgtcaaa acttaaacgc tgttgctcaa aaaccatccc tttcaacgca
    23761 tggagagaat cgctcaggtt ggcgataccg atcccctgta cgccggagaa gttataacgc
    23821 gcgccgccat cggtaatatc gctgcctttt tccagacaat cgctgataaa cgatgagagc
    23881 agcggtacag gtgcccagtc acggtgaccg atatcgcaaa tattgctgcc ctcaaccatc
    23941 agggtgatgt agtggctgat cttggcgcgg atctgctcca gtaaaccttc ataagtcagc
    24001 gcagcattgc cttcattttc atgcaggcag atttccatca ctttcagcag attaaacatc
    24061 gcgatgtcat gcaagccgta ggttctgccg ggaatagata attccacaca gcccactacg
    24121 gaatagtcgc gtgcatcttc cagcgaaacg ccacggttga ggaacgctgg caccaccact
    24181 tcatcgttaa agatttgcgg aataccggtg ccgaggcgaa tggtttctgc ggttttcatc
    24241 aggaacggca tgtcgatcaa ggcgttagtg cgcacgccga ggttcggttg cggtaattgc
    24301 acgctttgat aagcgtcaag gcaaaggaac gaaagcacgt tcaccgcgct acgtccgttc
    24361 tcggttaacc cgccgagcag tgcggtatag ccggtcggga aaccggcgaa ataacgcgcg
    24421 ctactggtgg agcgcaacag cacgatgtcg ttgcatttca cccataaaga ttcgagcagt
    24481 tctttcagga acgccggatc ttcgccctgg gttaatgatg cctgatagaa cggcaacata
    24541 tactggtcga agcgccccaa cgatagtgaa ctggcgttgg attcgtattg cagaatgatg
    24601 ttcatgtacc agaataactg gcacgcctgc cagaacgtct gcggcttatg ttcggcgtta
    24661 tggcgggaga tctccgcaat agtcagcagt tcttcgcgac gctgggcatc agtgcagctt
    24721 gccgccatcg tttccgccag ttcggcgtaa cgcaaaatgt gtttctgcga ggcttcgagc
    24781 agtaacagcg ctgcctgata aaagtgattc tccggctgtt gctgacaatg ttgctgcatc
    24841 tgcgctacca gctcacccag cccgtgattc agcaggcgcg ggtaatcaat aataatgtgc
    24901 ccctggcctt tatccgtctg gttgatgctg aaaatctgcg tgctggtcgc ggcttttact
    24961 tcatccgtca tctgcccgtt aatgaaatct ttcatcgaac gtttttccca gtacgggaac
    25021 aactcttcac ggtagatacg tttatcttct tcgctgatag caaagcggtc ctgcggacgc
    25081 gtcgggaatt gatccagctc tttcagcagc cagtaagggt ccatttccgg cgacataatc
    25141 ccggcgcgcg gttttacggt gcggttaccg gcaatcagtt cttcatcacg aatcgaaatt
    25201 tcaacatgtt caaggatata cgctgttgct ttcgcccggc gcaatatcac cggttcgcct
    25261 tcggtttgcc gatggctggc ggtataaagc agcgcacgct ccagcgagat ttcacgggta
    25321 ttggcaaaca gtgcagtttt gaggcgagag atacgattcg tcatgaggaa ctcctgtggg
    25381 aaagcgaacc cgcccttacg ggcgggataa gaacttaagc ggtttgtgcc aggtgagctt
    25441 caattttgct cataatggca tcagcgcgtt ttaccgcgtc gctgatgttg acgcgcacga
    25501 tggtcttgcc agcaaaacgc tcttcaaact tgatgccgat atctttggtc aggatcacca
    25561 tatcggcgct cgccacgtct tccgcagtca gttcgttttc cagaccaatc gagccctggg
    25621 tttctacttt cacttcccag cctttcgctt tcgcggcact ttccagcgcc tctgccgcca
    25681 tataggtatg ggcaacacca gatggacacg cggttactgc aataattttc gtcatggctc
    25741 gttcctcaat taattaattt caaaatccaa atccaggtcg tcttcttttt catcagtcga
    25801 agacccattt ttacgcgcca gacttttcag cacgttcaca caaacagccg tcaccactgc
    25861 tccgacagct actgctgcga tataacccag cttgccttca accaccggca gcacaatcag
    25921 accgccccag cctgcatagc actgcgcacc gaccagcgcc gcagttactg caccacaaac
    25981 tgaaccgacc atgatggaag gaataacacg cagcggatcg gcagcggcga aaggaatcgc
    26041 cccttcagta accccaacgc accccatcac cagcgccgct ttaccggttt cgcgctcttc
    26101 tgcggagaaa tttttacgac caatcagcgt cgccagcccc atccccagcg gcggaataca
    26161 aatacccacg gcagcaatgg ccaccacggt ataaacaccc tgagcaacgc aaatcagcat
    26221 gaaggcatag gccactttgt taaccggacc gcccatatcg aacgccagca tcaggcccat
    26281 gatcaccgcc agcataacaa tgctgccctg ctgcatcccc tgaagccact gagtcaggct
    26341 gttggtcaat gcccccacag gctcgcccag cccccacatc atgatgcctg cggtaatcag
    26401 tgtgccgacg atcggaatga tgaagattgg catcaccgag cgcagaactt tatgcaccgg
    26461 aattttcttc aggtaatgca ccacgatgcc gccgataatc ccggcgatca gtgcaccaaa
    26521 gaagcccgca ccaaagctgt taccaaccca ggcaccgata gcgcacggag ccagcgcaga
    26581 acgctctgca atggagtaac caatgtaagc ggcgaggaaa ggcaccatca gcgtcaaacc
    26641 cgcaacgccg atatcaaaca gttttttcag attcggatcg gctacggcat ccggcactgc
    26701 gcctttgcca tacaacatga cggaaaccgc cagcaaaata ccgcccgata ccacgaaggg
    26761 aatcatgtgt gaaacgcccg tcattaaatg ctgacgggta tttttcagga tctgcaccaa
    26821 ctcattcata agctgctccc gggcttgcaa aagcccatgt ccatattttt gatggaggaa
    26881 acaataggca atcgcaggcg gagcaaacag tggataaaaa ggccatttac tggacttttg
    26941 taatatcagt acaaaaatgc gatccgcctc ataacttgcg atgatccgga agcatgccgc
    27001 aaaacggcgt cttgtgagac gcgtcgcaaa ttgacggtta cattacattt gtccagcttt
    27061 tggcagattt gtcacatggc aggccgttgc cgtgcgctct tatcctgtaa acagaaaacc
    27121 gtaccgggag agaggcaatg gccctgattg tggaatttat ttgtgagcta cctaacggcg
    27181 tacatgcacg tccggcaagc cacgttgaaa cgctgtgtaa tactttttca tcacaaattg
    27241 agtggcataa cctgcgcact gaccgcaagg gcaacgccaa aagcgccctt gcgctgattg
    27301 gcaccgatac gctggtgggc gataactgcc agttactgat ttccggggcc gacgaacagg
    27361 aagcgcacca gcgtttaagc caatggctgc gcgatgaatt cccccactgc gacgcaccgc
    27421 tggcggaagt aaaatctgac gaactggaac cactgccggt ttcactgacc aatctgaatc
    27481 cgcaaattat ccgtgctcgc accgtgtgca gtggtagtgc aggcggcatt ctgacgccga
    27541 tctcttcttt agatctcaat gcgctgggta atcttcctgc tgccaaaggc gttgacgccg
    27601 agcaatccgc gctggaaaac ggcctgacac tggtgatgaa aaacattgaa tttcgtctgc
    27661 tggatagcga cggtgctacc agcgcgatac tggaagctca ccgatccctg gctggcgata
    27721 cttccctgcg cgaacattta ctggcaggtg tcagcgccgg attaagctgc gccgaagcga
    27781 ttgttgccag tgcgaatcac ttttgcgaag agttcgcccg ttccagcagc agctacctgc
    27841 aagaacgtgc cctggacgta cgcgacgtct gcttccagtt actccagcaa atctacggtg
    27901 agcaacgctt cccggcaccg ggcaaactga cgcagcccgc catttgtatg gctgatgaac
    27961 tgacccccag ccagttcctc gaactggata aaaatcacct caaaggtcta ttactgaaaa
    28021 gtggcggcac cacctcacat acggtgatcc ttgcccgttc gttcaacatt ccgacgctgg
    28081 taggtgtgga tattgatgcc cttactccgt ggcagcatca aacgatttat atcgacggca
    28141 acgcaggggc gattgtggtt gagccagggg aagccgtggc ccgttattat cagcaagaag
    28201 cccgcgtaca ggacgccctg cgtgagcaac agcgtgtctg gctgacccaa caagcccgta
    28261 ccgctgacgg tatccgcatt gaaattgccg ctaacatcgc tcactccgtg gaagcgcagg
    28321 ccgcattcgg caatggtgcg gaaggcgttg gtttattccg cactgaaatg ctctatatgg
    28381 atcgcaccag tgcaccgggc gaaagcgagc tgtacaacat tttttgtcag gcgctggagt
    28441 ccgccaacgg acgcagcatt attgtgcgca ctatggatat tggcggtgac aaacccgttg
    28501 attatctgaa tattcccgca gaggcaaacc cgttcctcgg ttatcgcgcg gtgcgtattt
    28561 atgaagaata cgcgtcgttg tttaccacac aactacggtc gatcctgcgc gcctctgctc
    28621 acggcagcct gaaaatcatg atcccgatga tctcctcaat ggaagagatc ttatgggtga
    28681 aagaaaaact ggcggaagcc aaacagcaac tgcgtaacga acacattccg tttgatgaga
    28741 agattccact cggcatcatg ctggaagtgc cgtcggtgat gttcatcatc gatcaatgct
    28801 gcgaagagat tgatttcttt agtattggta gtaatgacct gacgcaatat ctactggcag
    28861 tggatcgcga taacgctaag gtgactcgtc actacaacag cctgaatcca gcattcttgc
    28921 gggcgctcga ttacgccgtg caggcggtgc atcgccaggg caaatggatt ggtctgtgcg
    28981 gtgaactggg agcgaaaggt tccgtgctgc cgttgctggt cggtttaggg ctggatgaac
    29041 tcagcatgag cgcaccatca attccggcgg cgaaagcgcg gatggcgcaa cttgatagcc
    29101 gtgagtgccg caagttgctc aaccaggcaa tggcctgccg tacttcgctg gaagtggaac
    29161 acctgctggc gcaattccgc atgacccaac aagacgcacc gctggtcacc gccgagtgca
    29221 tcacactgga aagcgactgg cgcagcaaag aagaagtgct caaaggcatg accgataacc
    29281 tgctgctggc gggccgctgt cgctatccgc gtaaactgga agccgacttg tgggcgcgcg
    29341 aggccgtttt ctctaccggt ctgggcttta gttttgccat tccccacagc aaatcagaac
    29401 acattgagca atccaccatc agcgtggcgc gtctggcagc gccggtgcgc tggggcgatg
    29461 atgaagcgca attcatcatt atgttaaccc tgaacaaaca tgctgcgggc gatcagcaca
    29521 tgcgcatttt ctcgcgcctc gctcgtcgca tcatgcacga agaattccgt aacgcgctgg
    29581 ttaacgccgc ctctgccgac gctatcgcca gcctgctgca acatgaactg gaactgtaaa
    29641 aggaaacatc atggaactgt atctggacac cgctaacgtc gcagaagtcg aacgtctggc
    29701 acgcatattc ccgattgccg gggtgacaac taacccgagc attatcgctg ccagcaagga
    29761 gtccatctgg gaagtgctgc cgcgccttca aaaagcgatc ggtgatgagg gaattctgtt
    29821 tgctcagacc atgagccgcg acgcgcaggg tatggtgaaa gaagcgaaac gcctgcgcga
    29881 cgctattccg ggcattgtgg ttaaaattcc ggtaacctct gaaggtctgg cagcaattaa
    29941 aatgctgaag aaagaaggca ttactacgct gggaaccgca gtgtacagcg cagcgcaagg
    30001 attactggcg gcgctggctg gagccaaata cgttgctcca tacgttaacc gcgtagatgc
    30061 ccagggcggt gacggcattc gtactgtaca ggagctgcaa gcgctgttgg aaatgcatgc
    30121 gccagaaagc atggtgctgg ctgccagctt taaaacacca cgtcaggcgc tggactgttt
    30181 actggcagga tgtgaatcca tcaccctgcc cttagatgta gcgcaacaaa tgctcaacac
    30241 ccctgcggta gagtcagcta tagagaagtt cgaacacgac tggaatgccg catttggcac
    30301 tactcatctc taaaggagca attatggacc gcattattca atcaccgggt aaatacatcc
    30361 agggcgctga tgtgattaat cgtctgggcg aatacctgaa gccgctggca gaacgctggt
    30421 tagtggtggg tgacaaattt gttttaggtt ttgctcaatc cactgtcgag aaaagcttta
    30481 aagatgctgg actggtagta gaaattgcgc cgtttggcgg tgaatgttcg caaaatgaga
    30541 tcgaccgtct gcgtggcatc gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg
    30601 gcggaaaaac tctcgatact gccaaagcac tggcacattt catgggtgtt ccggtagcga
    30661 tcgcaccgac tatcgcctct accgacgcac cgtgcagcgc attgtctgtt atctacaccg
    30721 atgatggtga atttgaccgc tatctgatgt tgccaaataa cccgaatatg gtcattgtcg
    30781 acaccaaaat cgtcgctggc gcacctgcac gtctgttagc ggcgggtatc ggcgatgcgc
    30841 tggcaacctg gtttgaagcg cgtgcttgct ctcgtagcgg cgcgaccacc atggcgggcg
    30901 gcaagtgcac ccaggcggcg ctggcactgg ctgaactgtg ctacaacacc ctgctggaag
    30961 aaggcgaaaa agcgatgctt gccgccgagc agcatgtagt aaccccggcg ctggagcgcg
    31021 tgattgaagc gaacacctat ttgagcggcg ttggttttga aagcggcggg ctggcggcgg
    31081 ctcacgcggt gcataacggc ctgaccgcta tcccggacgc gcatcactat taccacggtg
    31141 aaaaagtggc atttggtacg ctgacgcagc tggttctgga aaacgcgccg gtggaggaaa
    31201 tcgaaaccgt agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg
    31261 atattaaaga agatgtcccg gcgaaaatgc gaattgtggc agaagcggca tgtgcagaag
    31321 gtgaaaccat ccacaacatg cctggcggcg cgacgccaga tcaggtttac gccgcactgc
    31381 tggtagccga ccagtatggt cagcgtttcc tgcaagagtg ggaataacct actccaaact
    31441 cccggcttgt ccgggagttt gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta
    31501 cgcaatctct gcaatatagt aaatttacgt acttttgtag gccggataag gcgttcacgc
    31561 cgcatccggc acgctgtgcc agtaatctga aacttccacc ttgttcgggt gtttttttat
    31621 ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga atctgtgact
    31681 cttattttca cggacgaaac agatgaaagc ttcactggcg ttattcagtc ttctgacggt
    31741 atttaccagt tactccttaa aatctcccgc cgttccacca accgtggtac aaatctaggc
    31801 caacaccaat cttgccatcg cagacggcgc cagacagcaa atcggcagca cgctatttta
    31861 cgatccggcg tatgtgcagc ttacctatcc tggcggtgat gtcccgcaag aacgcggcgt
    31921 atgttccgat gtggtgatcc gtgcattgcg cagccagaag gtcgatttgc agaaactggt
    31981 gcacgaagat atggcgaaga attttgctgc gtacccgcaa aaatggcagt taaagcgccc
    32041 ggacagcaac atcgatcacc gccgggtgcc taatctggaa acctggttta cccgccacga
    32101 taaaacacgc cccaccagca agaaccccag tgactatcaa gcgggcgata ttgtctcctg
    32161 gcgattagat aacgggctgg cacatattgg ggtggtgtca gatggcttcg cccgcgacgg
    32221 tacgccgctg gtgatacata acattggcgc aggcgcgcag gaagaagatg tactgtttag
    32281 ctggcggatg gtggggcact atcggtattt tgctaaataa gagatctccc cacatgaaga
    32341 aaaaattcag atccttttta cactgacggc ttcttttttc ttacggctca ccagcgccag
    32401 tgccagtaca atcagcacaa taccgctacc ttcaactgcc cccggattct caccgagcag
    32461 ccaccaggaa aagagtacgc cgcaaacggg aacggccagt gtgcttaagc tggcaatact
    32521 ggcaggcaag tttttcaata caaacaacca taagctccac gccagcgctg tcgccagaat
    32581 cgcactgtag gccagcgccc agaacacggt gggttgccag tcaatttcac gttgcggcac
    32641 cagtaaagcg accacgctca tcaccagcgc cgcatacagc atctgccagg atgttagcga
    32701 caataaatcc acgcgcggat ggcgggcata cagacgttta gcaacaatcg cgctcgcccc
    32761 ccaactcaca ccggagagga ttgccagcat ggcgcttttc atcgaagaga aatcgagttg
    32821 ccacggctgc aacaccaaaa ataagccgaa agcggcaatc agaatcgcga aatattgtcc
    32881 gcgtcgcagg cgttcaccga gaaacaacgc ggcgaaaatt accacccaga acggcatggt
    32941 atagctcagg attgccacct tccccgctcc tccgctgacc agcgcccact gcgccaggcc
    33001 gaccatcccg caggtttgta acagggcaat ggctaaggtg tatttaaacg gtgtcgggcg
    33061 cattccgcga ccacgtaata aaaggacgat gaataaaacg agagcgccga aaatgcagcg
    33121 taaggcggta aagtcgaagg caccgatgta gctggtgact tgcttcatga aaatccagct
    33181 ataactccag ataagtgtga gcacaaccag gccactgatt gccagtgggt tgctctttcc
    33241 tgcggcagac atgagagatc cagtgtgtag ttttcctgtg gacactatac gccggtaaag
    33301 aaacttcagc gactgcatga aaggcagtcg ctgaagaagg tcagattaca gcaggtcgaa
    33361 acggtcgagg ttcatcactt tcacccacgc tgccacgaag tctttaacaa acttctcgtg
    33421 ggcatcgctg ctggcgtaga cttccgccac cgcacgcagg acagagttag aaccaaagac
    33481 cagatccgca cggctggctg tgtatttcac ttcgccagtt tcgcggtcac ggccttcgaa
    33541 cagctctttc gattcgtcgg tcgctttcca ctcgtaacgc atatccagca agttcacgaa
    33601 gaagtcattg ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc tgccatcgaa
    33661 gttggcaccc agtacgcgca tcccgcccac cagcgcagtc atttccggcg cggtcagcgt
    33721 cagttgctgt gctttatcaa tcaacagtga ctcggtggtg gaaacgtcca gacgagcgcg
    33781 atagttacgg aaaccgtcag caattggctc cagcagttcg aacatctcaa tgtcagtctg
    33841 atcctgacgc gcatcaacgc ggcccggcgc aaacggtaca tgaatgctca aacctgcggc
    33901 gcttgcggct ttctcaacgc caaccacacc tgccagcacg atgatatccg ccagcgaagc
    33961 tttgccagac tctttctgaa ttttctccag aactggcaga gcacgaacgg ctgcggcgtt
    34021 cacatcccag tcacgctgcg gcattaatgc cagacgcgca ccgttggcac caccacgttt
    34081 gtcgccacca cggaaggtag aagcagacgc ccaggccacc gataccagct cactaacaga
    34141 cagaccagaa tccgcaatcg cgaatttcag atcgataatg tcctgctcgg tcgggttgta
    34201 gatcggctgc ggcagcggat cttgccagat cagatcttct ttcggtactt ccggcccgat
    34261 gtaacgagat ttcggcccca tatccctgtg cgtcagtttg aaccaagcac gagcaaaggc
    34321 ttcgttgaac gcctgcggat cgttgaggaa acgacgagag atcttctcga actcaggatc
    34381 aaaacgcagc gtcaggtcgg tcaccagcat cgtcggttta cgtttcttcg acggatcaaa
    34441 cggatccggg ataatttccg gtgcgtctac cgcttcgaac tggattgcgc cagccgggct
    34501 gcgagtttgt acccactcat atttgaacag gttctcgaag aaatagttgc tccattgggt
    34561 cggcgtctgg gtccagacta cttccagacc agaggtaatg gcatctgcgc caacgccgct
    34621 gccgtaagtg ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt ctggatcagg
    34681 acctacattt gatgtcggac cggcaccgtg agttttaccc agcgtatgac cacccgcaat
    34741 cagcgccacg gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc ggatagctgc
    34801 tgccgcagaa agcggttcgc cgctgtgatc cgggccttcc gggttaacgt aaatcaggcc
    34861 catctcggtt gcgcccagcg gtgctttcgc cagtgcttcc ggatgacggt gggtcagcca
    34921 ggctttttca tcaccccagt taacatccag atccggttcc cagacgtctt cacgaccggc
    34981 accaaaaccg aaggtacgga agccggagtt ttccagcgcc acgttacccg cgaggataaa
    35041 caggtcggcc caggagattt tctgaccata tttctgtttg attggccaca acaggcggcg
    35101 cgctttatcg aggcttacgt tatccggcca ggagttcagc ggtgcaaaac gttgctgacc
    35161 acgacccgcg ccgccgcgcc catcgattga acggtaagta cccgcaccgt gccaggccat
    35221 acgaataaac agaccggcgt agctgcccca gtcggcaggc caccacggtt gagattctgt
    35281 cagcagggct ttcagatctt ttttcagtcc gtagtaatct aatttgctga attctttgcg
    35341 gtagtcaaag tcctcaccca gcgggttaga acgattagaa tgttggttta acaggtcaac
    35401 acggagttga tttggccacc agtcgcgcgt ggttgtgccc ccacctgcgc tctggtcgtg
    35461 accgccctga tggaacgggc atttgccggt ggctgtcgtg ttatggatat cgtctgacgt
    35521 gctcatcaat gtgctcccct ctacagtgtt accgttacga tacacagcgt tagagagaag
    35581 ttataattga attaatccac ggatgcgata gttgagatct tacatattgt tggttaaaga
    35641 gatgtagatc aaattgatcg taattaagac tttttgatta tgtccgattt aggacaaatg
    35701 ctttataaaa aagggtttta gatttgtatc cttcaagtaa tgagggagaa aacaaggctg
    35761 ataaccttat tttcccgccc tcatttcgag gcagcatttt gtgctctgtt taaaatttgt
    35821 gatcactgtg taattttcac aaaaggcaca ctatttataa accaggtcga acacccagtg
    35881 tatggcaaat cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg aaatctttca
    35941 ctccttcacg gcttaaaatc ttcaccatat ccatggcaat attcgcgccc accagcttgc
    36001 gggtttcagc atcgtcatcc aggccgtcga acatttgtgc catccacgcc ggaatacgca
    36061 cgttggtcat gtcggcaaat ttcttcgcct gtttaaagtt cgaaaccggc aaaattcccg
    36121 gaatgatttc cacatcaatg cctgccgaga cgcagcgatc gcgaaaacgc aggtagcttt
    36181 cgacatcgaa gaagaactga gtaatggcgc ggttggctcc ggcatccact tttcgtctca
    36241 ggttgagtaa atcggcctgg gcacttttcg cttctggatg tacttccgga tacgccgcca
    36301 cggagatatc gaaatctgcc acttctttta acagtgtcac caggtcagaa gcatacattt
    36361 ctggcttacc acttcccggc ggcaggtcgc cacgcagcgc cacgatatgg cgaataccgt
    36421 tattccagta gtcgcgtgca atggtccgca gctcggcagg cgtcgcatca atacaggtaa
    36481 gatgcggtgc cgcttccagg ccggtgcgat ctttaatgcc tttaataatg ctgtgcgtac
    36541 ggtcgcgctc gccggagttt gcgccatagg tcaccgatac aaacttcggt ttcaggctgc
    36601 taaggcgatc gatggagttc cacagggtct gctccatttc actggtacgc ggcgggaaaa
    36661 attcgaacga aacgttaatc tgcccctgga cttctgccag actctgattc agggcatccc
    36721 gctggctggc gtgaaaaaag ctcatacctt acctcatcaa tcgcttatcg ttatatgttg
    36781 tgttgtgcac atctatccgt ttagacgtcc agatgtaaag atgaaggaaa agccgaaggg
    36841 cgtcaaccga aaaataacca atgatggtga ggaaaaatca gggaagatga aaaaaactca
    36901 tcttaattag aggcgataaa agatttctct atagcacaat ccacctcgca cgttttgact
    36961 catattctga caacacagcg acacctgggc taattcgaaa aacaacgtcc tgccaccgga
    37021 tggtgatgct aaggcatctg attcggcctg cgccctcgcg taggccgaat acaggatctg
    37081 cgcaatcaac gggtaacaaa cagttgagtc aattcagtgc gcacttttat ttgatatcct
    37141 tcacgcgcat accgttgagc tgttcatccg taatggtgtt gcccgctttg aagtaatcaa
    37201 ccaccgcgtg ataaacgtaa taaccgcccg ttgtgttacg cgctttccct tcggtaaagg
    37261 cggtaaaacc atctccacca tcagcaagga aactctgagt agcaatgtga taaaccgtcg
    37321 catcttcaat gggtttgcca ttcagagtca gtgtgattac ccgctgacca accggcttgc
    37381 tgctgtcgta cttcatttcc aggcctttcg atacctgtaa aacgccattg cttaaacttg
    37441 cgccgtgttc catcagactg cgtaattgtt tacccgtgag ttccatcgtc accagttcgt
    37501 tggggaacgg gaacgtactg atgacgccgc ccatcgtgat cgcccctgcg gggatctcat
    37561 tgcgaatgcc gccagagttg gttaacgcca attgggtgtt tttacccgcc gctaccagta
    37621 aagcgtcagc cgccaggttc ccgagggaag ccgattcacc ataggcacgt tttaattcaa
    37681 ccggcgattg cgccaccgtt tgttgcacca cttcatcaag ctttttattc cagccgtcga
    37741 tcacctgttt cgtttgtgga tcgggcttcc actcgtcagc gtaaatggtt ttaagctcga
    37801 agtttttcac cgtaaaatta tgcggcttct ctttgtagtc gagaaccagt ttgcccacat
    37861 caatcccgcc gctgtcagtg gagaggatga gcgtattgcc gactttaatc ggttccggcg
    37921 tacccacatg tgcatgtccg gtgatcaaaa tatccagccc tttcacctga cttgccgtct
    37981 gaatatcttt atccagcgcg cgacgcacat ctgtgcctcc catactggac tggcgggccg
    38041 gaacaccttc atggatcagg gcgacggtca aatcaacctt gcctttaagt tcatcgatat
    38101 aacgttgtag ccatttaatt tcatcacgcg cctcaatacc cactcgcgtg gcagcagata
    38161 ctgtgtcatt aaaggcaaat acaccgtgca aacctataac gcctattttc acaccgtctt
    38221 tttcgatgat ggtatagggc ttatcccaga atgatttact gctgttctga taaaaaacat
    38281 tgccctgcac aatcgggaat tttgcctgac tcaactgtaa taatgtgttg tcccagccgt
    38341 ggtcaaattc atggttgcct atcgtgacgg catcaaacgg catagtattc ataatatcga
    38401 taatagcctt acctttggtc aggctgctga tatatggccc ggtaaaatag tcgcccgcat
    38461 caaaaaacca tgtcgcttta ttttttgctt tttcttgctt aaccagcgta gtgatatttg
    38521 cccagccgcc aatatcccgt ttaccgtcag cgatccacgg cactttataa ggttctacat
    38581 gcgcatggag gtcgttggta taaataatag tgacatcttt agcgcaagcc cagaacggca
    38641 gtgttaatgc gatacccgca gcaagtattt taattttcat aaacaatccc tttcagtgag
    38701 ataaaggatt gctacattac gttctgaaag acttatttat gtgagagtaa tcacatctgc
    38761 aggggatagt tgccggacaa aatcaacgac cttgtgaaat cgttggtgat atatgtcgac
    38821 tctgtttacg attaatgcct gtaaaacgtt cggttgccga aatttgggat tggcctcatc
    38881 ggaagattac agttggccag actataaact gggttatccg gcactgcact gtcgggcatg
    38941 tggcagttac ccgccgttgt ttgacgagca acaatttcgt gactggttgt cagttcactt
    39001 atctacgtgg gcctcggaga agggtcattt ctgcccggta tgttatggca cagacatgat
    39061 ttgttatggt cacaatccac agggttcgca acgcattcaa tgccgtaact gcaaaaaagt
    39121 ctggacgcct aaacagtatc agaaagaaat tacgcatccc caggccattg aaaccgttca
    39181 actgttcata ccctttcaag gggccagcgc agtacaaaaa ctgtatgttt tagtcagtct
    39241 tgacgccact cgtggcaaca ttcttcatct ctccacgaat tatacgcaac atcagacagg
    39301 agacagtctg cggtacagtt acaagggcaa cacagaacca actatgcacc atcgcgatat
    39361 cgtgcagaag gtagatatgc gtgaagcgca attcttgcgc cggagccagt tcgatgaaat
    39421 tcagtatggc agtgctgtac tcaagcgcaa cggtaaggga gccatactac gcccggttat
    39481 cacggcacac gggcatttca gaatactgaa aatccgctat ccagatgtca aaacgcatat
    39541 tatttcacac gaatgttttc taagaggcgc aattattaca gcctgggcag atcagttccg
    39601 ccaacaacaa ggtgaacttt ggttcgtaga agaagaaatc agtgacagta acgctgatac
    39661 gccctggcat tttaagggaa cgacatacca tggttggtgg caaaatcagt ggcagcgctg
    39721 ggagcagggg aataactgca agatggtctg cctactcacg ggagcctgct tagaaagggg
    39781 cgcaaatgtt tctctggcaa ccagtcgttg ttttatcaca tggctgacag accaacacga
    39841 ctttacccaa agcgcgttat tatccgcagg tcgcgtaaca caaatgctga cctcactggc
    39901 gttaaaatac aatgaatcgc tcactccatc ttgttagtca atacactcga caaacgacca
    39961 taaattaaga gagaacacta cctttcattt aattaaatat ataaatgtat gaccgtgaaa
    40021 tataatcatg gtcaatgttt aattaataac attatttgtt actaagatca cacaatactt
    40081 ttctgaacta tgcttacata aaataagaca cgatgtgtct tttatttaaa taattcaaca
    40141 aatgtaacac accctatcca ggagggatga tgaaaactgt atttacaaca ttatcagtca
    40201 tattcggcgt gatattttct cacagtgttc tagctcagga cgttactatt tattatacga
    40261 atgacattca cgcacatgtc aatccagcaa aaatcatcgc tgttgataag aacagactta
    40321 taggtggtat ggctaatatt tcaggcatcg ttaatgaagc gaagaaaaaa agcaaagatg
    40381 tgtttttctt tgatgcaggc gactatttta ccgggccgta tatcagcacc ctgaccaaag
    40441 gtgaggctat tattgatatc atgaacacta tgccctttga tgcggtgtct gttggtaatc
    40501 acgagttcga tcacggtgtg cccaatatgg tgtctcagtt atcaaaagca aaattcccca
    40561 ttctgctggg aaatatctac tacaccgata cgaataaacc agtttgggat catccgtgga
    40621 ccattatcga aaaagatggt ttaaagattg gtgtgattgg attgcatggc gcattcgctt
    40681 tctatgatac agtcgcggca aaagcacgtg aaggcgtaga agccagagac gaaattaaat
    40741 atttaaataa agcacttgca gaattaaaag gtaaagtcga cattaccgtt ttgctaattc
    40801 acgaaggtgt cccggcgcgt caatcaagct ttggtagtaa agatgtggag cggctactac
    40861 aggcagatat tgagacagct aaaaaagtga atggtgttga tgtcttaatt accggtcacg
    40921 cacatgtcgg aacaccgcaa ccaataaaag ttaataacac attaattgta tctaccgatg
    40981 catacggcac caacatcgga aaacttgtgc ttgattacaa tccgaaaacg aagaaaattg
    41041 acagttataa tggtgagtta atcaccatct ttgcagatca atttaaacct gacaccatcg
    41101 ttcaaaatac tattgataaa tggagtgcaa agcttaacaa aataacacag gaagttgtcg
    41161 gccattctcc cgtagtttta acacgtgagt atggtagttc ctcttctacc ggcaacctta
    41221 ttcttgatgc aatgatggaa aaaacacctg atgccgttgc cggatttcaa aatagcggtg
    41281 ggattcgagc tgattttcct aaaggcgata tcacactggg agatgttatt agcacattcc
    41341 cctttaataa tgacctcatc gagatggatt tgacgggccg cgatctcaaa tcattgatga
    41401 cgcatgcaac caatctaact aacggtgtgt tacaagtttc taaaagtgtt gcggttgtct
    41461 atgacagcaa aaaacccctc aaccaacggt taatctcttt caccattaac ggcaaacccg
    41521 tggaagataa tcaaacatat cgtattgcca cgcactcctt ttgtgccagt ggtggtgacg
    41581 gttttgaagc atttttgaat ggaaaaaatg tgaagacgat accgggaaca acctcggcgg
    41641 aatctatcat cgattatttc aaaaatcata aacctgtcac cccagactta actaaacgag
    41701 tcatggacgt cgccaaataa aatctcattt atcagtcaat ttatttaaca aggaatgcga
    41761 atgaaaacag cgcagcgtac tttatttgcc gcaacattgc tgactatttt ttcgacctca
    41821 gttatggcgc aggatgtcac gattatttat accaatgatc ttcacgctca tgtggattcc
    41881 tataaagtac cgtatgtcgc agacggaaaa cgcgacattg gtggctttgc caatatatcc
    41941 acgttagtaa aacaagagaa agccaaaaat aaagccacct tttattttga tgctggagac
    42001 tatttcaccg ggccatatat cagtagcctg acaaagggcg aagcaataat tgatattatg
    42061 aataccatgc cttttgacgc agtatcaatc ggtaatcatg agtttgacca tggctgggat
    42121 aatgccctcc gacaattaag caaagcgaat ttccctgttt tattagccaa tgtttatcat
    42181 aaagagagtg agaaaccctt ctggaacaag ccctacacca tcctggaaaa agatggcatc
    42241 aagattggta ttgttgggct acacggcgta tttgcattta atgacacggt ctcggagctc
    42301 tctctccagg gactcgataa tgacaataat aaccgatttg ataaatcttc cgcgaccctc
    42361 aagaatcagg ggattgaagc acgtgatgaa gtgaagtatt tgcagcatta tatcgatgag
    42421 ttacgtgata aggttgacct caccgtggcg ctggttcatg aaggcgtacc ggcgcgtcag
    42481 tccagcattg gcaatacaga tgttagacgc gctcttgata aagatattca aaccgcaagc
    42541 aaggtgaaag gattggatat tctgattacc gggcatgccc acgtcggaac gccagaacca
    42601 atcaaagtcg gcaacacctt aatcctttcg actgacagtg gcggtatcga tattggtaaa
    42661 ttaatccttg atgtcaaccc tacagctcgt actcataaag tgaagagctt tgagctgaaa
    42721 acggtttatg ccgatgaatg gatacctgat ccaaccacac aaaaagttat caatggctgg
    42781 aataaaaaac tggcggatct tgtacgccaa ccagtcggtg aatcatcgat tgcattaact
    42841 cgtgcctacg gtgagtcatc gcaacttggc aacctgttta ccgatgcaat gcttgtcgct
    42901 gcaccgactg cacagattgc attgataaac tccggcagct tacgtgcgga tataaatgca
    42961 ggcacgatta cctttggcga cattaccagt acattcccct tcaagaatga acttactgaa
    43021 atggatctca gcggtaagga tctgcgtaac ctactggaac acggggcatc gcttaccaat
    43081 ggcatcctgc aaatgtcaaa aggcgcagaa atgcgctata ccccgaagaa accagtaggt
    43141 cagcgcatag tatcttttaa aattaatggt gaagagatcg ttgatacgaa tatttaccat
    43201 gttgcaacaa cgactttcct tgcactgggg ggcgatggtt ttctggcatt caaagaaggg
    43261 aaaaatgttc aggtccgcgc cggaaataac atgtccgatg tcgtgatcga ttatttgaag
    43321 aaaggccaca agattacgcc tgcacaggta aatgaaatgc gggtggacgt tagcaaataa
    43381 taaaaaaatt ataatgccat tcattcactg aatggcatta tttttctcta aatcaacaat
    43441 aaataatgag atatatttat gtgtataaaa aagcacggga tagctttatc cattctatta
    43501 ataccttgca ttggaaacgc tcaggaaatt aaaattgatg aaagctggtt acatcaaagc
    43561 ttgaatgtca ttggtcgcac agactctcgc tttggcccaa gactgactaa cgacctctac
    43621 cctgaatata ctgtagcagg aagaaaagac tggtttgatt tttatggtta tgttgatcta
    43681 ccaaaattct ttggcgtcgg cagtcactat gatgttggga tctgggatga gggctcacca
    43741 ctatttacgg aaatagaacc tcggttttcc attgacaaat tgaccggatt aaatcttgcg
    43801 ttcggccctt ttaaagaatg gttcattgca aacaactatg tctatgatat gggtgacaac
    43861 cagtcatccc ggcaaagtac atggtatatg gggcttggta cagatatcga cacgggtcta
    43921 ccaattaagc tttctgccaa tatatacgcc aagtatcagt ggcaaaacta tggtgccgct
    43981 aatgaaaatg aatgggacgg atatcgattc aaaataaaat atagcattcc tcttacccat
    44041 ttattcggag ggcgattggt atacaatagt tttactaact ttgattttgg ctccgatctt
    44101 gcggacaaat cacacaataa taaacgaacc agtaatgcta ttgcttcaag ccatatcctt
    44161 tccctgctat atgaacactg gaaatttgca tttacactac gttattttca caacggtgga
    44221 caatggaatg ctggggagaa agttaacttc ggagatggtc catttgaatt aaaaaacaca
    44281 ggatggggaa cctatactac tattggttat caattttaaa tcaggtgatg ttttagatat
    44341 ttttacttaa tgccattttg taattgtcat aaataacttc accattcaca ctgtgaattc
    44401 atcgctcaac acaaaagccc ccttattacc ggggcactga ccaaaggcaa aatgacaatc
    44461 aatcaccacc tccgcaggag gtggtttaga ggtgacaaca aaagctggca actgccagct
    44521 tttgttcaat aatcactaca acaactgcgc cagacggtta atatccgact gaatcgcccc
    44581 ggcggtgacg tcgcgccctg cacctggccc gcggatcacc aacggattat cgcgatacca
    44641 gcggctttcg atggcaaaga cgttatcgca cggtagtagt gatgccagcg gatggtcttc
    44701 tcgcaccgct tccacgccta ctcgcgcttt gccgttggca tcgaaacgcg ccacgtagcg
    44761 caacaccagc cccatttcac gggccgcttc caaccgttgt accatctgct cattaagttc
    44821 atcgccattt tcaaagaaat ggtcgatgct tccaccttcg caatgggttg gcaccagtga
    44881 ttcaacacgc acctggtccg gttcaatgtt gtaacccgct tcacgcgcca gaatcaccag
    44941 cttgcgcatc acatctttgc cggagaggtc atcacgcggg tcaggttcag ttaagccctg
    45001 ctgccacgcc tgatccacca gctcagtaaa cggcacgcta ccgtcgaatt gcaggaacag
    45061 ccaggagagc gtgccggaga agatcccgct gatcgacaaa atagtgtcgc cgctgtcgat
    45121 cagatcgcgc acggtatggt tgatcggcaa acctgcgccg acggtggcgt tgtacagcca
    45181 gtgacgcccg gttttttcga acgcgtcgtg gatctggcga tatttattgc tgtcgctcgc
    45241 tcccgccagt ttgttggcac tgatgacgtg gaaaccgtgg ctggcgaaat cgagatactg
    45301 atcagcaagc tgctggctgg cagtaacgtc cagcacaact aaatcatcat acggatgggc
    45361 acgcatccac aggaatagcg actcttcatc ctgttcgacg gcttcatcat taaagaaggc
    45421 tagcgcacgg ctggcgtcca gcccgtcata gctcaacagg ctgcgacggc tgtccaccac
    45481 gcctgccagc acaaattcaa agccggtgcg cgccgagagc gtgctctgct cacgggcgaa
    45541 cagttccagc cagcgggaac cgatatttcc cttaccgaac aataccaggc cgatgcgttt
    45601 ttctgcgcgg aagacggact gatgcagccc ctgaatcagg ctttcggtcg ggccggtgcg
    45661 cagtactgcc accaggctga tgccgtcatc ggactgccag gtaaactcga ccggctggcc
    45721 tttcagttgc tgccagaagc ggtggcaatg cagcgggtta cgggtgacgc ccgccccaac
    45781 catcgccacc agcgccagcc cctgacgcag acgcagttcg ccgggtaatc ctgcttcgtc
    45841 gaggattttc agcgcactgt cggccacttc cgaggtgtag caaaattgca gcaactggcg
    45901 atcgttatgc acgccaactg ccagtgggcg cacctgagca cgtttcagga tttggtcgat
    45961 ctctttatgc gccagtttaa aatcctgact ggcgggcacc tgaaactcaa tcaaacagac
    46021 atcatcgtgg ctggtgacaa tacgcgcacc agtaccggag gccagcacgc gttcaatgcg
    46081 cgtggaacct tgatccggcg tgtagctaca gcgcagctgc aggtcgattt cactaccgga
    46141 tactggctgt aaagtacggg cgtgaagaac gggggccgcc agacgcgcca gttcgctggc
    46201 ttcatcaaga cgcagcaatg gcagcagaca ggcatctttc actttacgcg ggtcggcact
    46261 atataccccg gcgacatcgc tccagatggt tacgcgggaa acacccgcca gcgcaccgat
    46321 ttgtgtcgcg gaatagtcgg aaccgttacg ccccagcagc accgtttcac cggcgttgtt
    46381 acggctgata aacccggtca ccaccagacg tttgcccgga tgttgtacca gtagctgttg
    46441 cagcaacggg taagaaagcc cttcatcaac ctgcggctgc gcggcgcgtt cagcgcgtaa
    46501 aaactcgcgg gcatcaagcc aggccgctgg cagcccttgt tgattaagta ccgcagacat
    46561 caggcgcgcc gaccacactt caccgtggcc caccacttcc gcatacaccg catcattaat
    46621 accgctgtcg agcagcgccg cgaggcgttc cagatcgctg acaaaggcgc taatgaggct
    46681 atcggcttct tcggcgggta gcaggccgct aatcagatcg cactgataac gacgtagcgt
    46741 ttgttgaacc tgatgcgcag agagacgatc ggtctggctt agtttcaacc agttaatcaa
    46801 ctggttagtg gtgctaccgg cggcggaaac caccatcata tcgtcaggct gagagtactc
    46861 cgccataatg cccgcgacac gcaaataaca cttcacatca gccagactac tgccaccaaa
    46921 tttatgcagt tgacgacctt tcgcccctgc ctgcgcaatc acactcattt ttaccccttg
    46981 tttgcagccc ggaagccatt ttccaggtcg gcaattaaat cttcgccatc ttcaataccg
    47041 gtggagatac gtagcagcgt ctcggagatc ccggcggcag cacgcgcttc tggcgccatg
    47101 cctgcatgtg tcatggttgc ggcgtgagag attaaacttt ccactccccc taatgattcc
    47161 gccagcgtaa acaacgacaa cccgcccagg aaacgacgca gcgtctgctc atcgccatcc
    47221 agttcaaaac tcaacattgc gccaaagcct ttttgctggc gcgcggcaat ttcatgcccc
    47281 tgattttccg gcagcgacgg gtgatacagt tttttcacca acggctgggt ttgcagatat
    47341 ttcacaatcg cctgcgcgtt gcgctgcgcc agctccatac gcggcaccag cgttcgcaac
    47401 ccgcgcagca gcagatagct gtcaaacgcg ccgcccgtca cgccaatatt gtttgcccac
    47461 caggcaagtt cagtgacaac gtccgggtct ttagcaatca ctacgccggc caccacgtct
    47521 gagtgaccgt tcagatattt cgtacatgaa tgcaacacca gatcggcacc taatgccagc
    47581 ggattttgta atgccgggct taagaaggtg ttatccacca cgctcaccgc cccgacttcc
    47641 cttgccagat ggcagatttt cgcaatatcc acgacgcgta acaatggatt acttgggctt
    47701 tctaccagta ccagtttggg tttttctgcc agcgctaccc gtaatgcctg ttcatcgcct
    47761 tgatcaacaa acaacacgcg atagcaaccg cgtttcgcca gactgtcgaa caggcgatag
    47821 ctgccgccat agcagtcgtg cggcgcaacc agcagatcgc ccggtttcaa aaagacggtc
    47881 gttaccagat gaatcgcgga catgccggta ttagtaagta cggcaccagc accgccttcc
    47941 agttctgcca gcgcacgctg aaccacatcg cgcgttgggt tgccgcgccg cgagtaatca
    48001 tgcgcgcgcg gttcattaaa tccggtaaag ttataggtgc tggaaagatg gatcggtggg
    48061 acaacgcaac catactgttc gtcgtcattt aacccgctac gcactgcgat ggtggcctgt
    48121 ttacgcgtca tgtgatgaag ttccctgggc ttggtcggtg aaatgtcagg caccagagta
    48181 aacattgtgt taatggacgt caatacatct ggacatctaa acttctttgc gtatagattg
    48241 agcaaatccc aaatagccgt taaaattata tgcattatca cgccgacagg tgcattacac
    48301 gatgtcacgg tagcgcctgt acggtaaact atgcgggttt acggtcagta cccacataaa
    48361 ttgtgtggtc tggtctcaat ttattgacga agaggattaa gtatctcatg gctgaatgga
    48421 gcggcgaata tatcagccca tacgctgagc acggcaagaa gagtgaacaa gtcaaaaaga
    48481 ttacggtttc cattcctctt aaggtgttaa aaatcctcac cgatgaacgc acgcgtcgtc
    48541 aggtgaacaa cctgcgtcac gctaccaaca gcgagctgct gtgcgaagcg tttctgcatg
    48601 cctttaccgg gcaacctttg ccggatgatg ccgacctgcg taaagagcgc agcgacgaaa
    48661 tcccggaagc ggcaaaagag atcatgcgtg agatggggat taacccggag acgtgggaat
    48721 actaaagcgg aaaagagcgg cgcggagtta accagccgct tttttcatac atggacgttt
    48781 aacgatgaaa atcaggctac tgatcctcag cctgctggtt tctgtgcctg cctttgcctg
    48841 gcagccgcaa actggcgaca tcatctttca aatctctcgc tcatcgcaaa gtaaagcgat
    48901 ccaactggcg acccattccg attacagcca caccggtatg ctggtgatac gcaacaaaaa
    48961 gccctacgtt tttgaagcgg tcggcccggt aaaatacacc ccactcaagc agtggatcgc
    49021 ccatggtgaa aagggcaaat acgttgttcg ccgcgttgaa ggcgaactga gtgttgaaca
    49081 acagcaaaaa ctggcgcaaa cggcaaaacg ttatctcggt aaaccgtatg actccagctt
    49141 ctcgtggagc gacgatcgcc agtactgttc ggaagtggtt tggaaggttt atcagaacgc
    49201 gctgggaatg cgcgtgggca aacagcaaaa gttgaaagag ttcgacctta gtaacccgca
    49261 ggttcaggca aagctcaaag aacgctacgg caaaaatatt ccgcttgagg aaacggtggt
    49321 ctcgccacaa gccgttttcg atgcgccaca actcaccacg gtcgccaaag aatggccgct
    49381 gttttcgtgg taatacgacc aaaattcagg cacaaaaaaa gcgccgtgcg gcgctttttt
    49441 cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac
    49501 ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca
    49561 cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc
    49621 agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt
    49681 ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag
    49741 cttcaatacg atcatttcgt atgaaggcgc gaaatcatac agaaattaac cagcgtatgc
    49801 aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg
    49861 atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttccccgt acctttgact
    49921 atctgctgcc agaaggcatg acggtcaaag ccgggtgtcg cgtgcgcgtg ccgtttggca
    49981 aacagcagga gcgcatcggg gttgtggtgt cagttagcaa tgtcagcgag ctgccgctta
    50041 acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact cactccgtct
    50101 ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc
    50161 atgccttgcc gattttacta cgccaggggc ggcctgcggc gaacgcgccg atgtggtact
    50221 ggtttgccac tgaacaaggt caggcggtgg atctgaacag cctgaaacgc tcacccaagc
    50281 aacaacaggc gctggcagcg ttacggcaag gcaaaatctg gcgcgaccag gttgccacgc
    50341 tcgaatttaa tgatgccgcg ttgcaggcgc tgcgcaaaaa aggtctgtgt gatttagcaa
    50401 gtgaaacacc agagtttagt gactggcgaa agaattatgc cgtttctggt gagcggttgc
    50461 gattgaatac tgaacaggcc accgccgttg gcgcaattca tagtgcggcg gataccttct
    50521 ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac
    50581 tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga
    50641 cgccgcaaac tatcgcccgt tttcgtgaac gttttaatgc gccagtggaa gttctgcatt
    50701 ccggcctgaa cgacagcgag cgtctttcgg cgtggttgaa agcgaaaaat ggtgaggcgg
    50761 cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggtgtgattg
    50821 tcattgatga agagcacgac agttcctaca agcagcagga aggctggcgc tatcatgccc
    50881 gcgacctggc ggtttatcgt gcgcacagcg agcaaatccc gattattctt ggctccgcaa
    50941 cacccgcact ggaaacgtta tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga
    51001 cgcgtcgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta aaaggtcaga
    51061 aggtgcaggc ggggctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggccg
    51121 acaaccaggt tatcctcttt cttaaccgcc gtggctttgc gcctgccttg ctgtgccacg
    51181 actgcggctg gattgccgaa tgtccacgtt gcgatcacta ctacacgctg catcaggcgc
    51241 aacagcattt gcgctgtcac cactgtgaca gccaacgccc ggtgccgcgc cagtgccctt
    51301 cctgcggttc cacgcacctg gtgccggtgg ggctgggcac cgaacagctt gaacagacgc
    51361 ttgcgccgct gttccctggt gtgcccattt ctcgtatcga ccgcgatacc accagccgca
    51421 aaggagcgct ggaacaacaa ctggcggaag tgcatcgcgg cggcgcgagg atcttgatcg
    51481 gcacgcaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg
    51541 acgtagacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc gcccagcttt
    51601 acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa
    51661 cgcatcatcc agaacacccg ctattgcaaa cgttgctcta taaaggctat gacgcctttg
    51721 ccgaacaggc actggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga
    51781 ttgtgcgtgc ggaagatcat aacaatcagc acgcgccgct gttcctgcaa caactgcgta
    51841 atctcatcct ctccagcccg ctggcagacg acaaactgtg ggttctcggt cccgttccgg
    51901 cactggcacc taaacgtggc ggccgctggc gctggcaaat tttgttgcag cacccttccc
    51961 gcgtgcgatt acagcacatc atcaatggta cgctggcgct tattaataca ataccggatt
    52021 cccgtaaggt gaaatgggtg ctggatgtcg atcccatcga gggctaatgc gctcacgatg
    52081 cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt
    52141 tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg
    52201 acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa
    52261 agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc
    52321 cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta
    52381 tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacta ttctggtgat
    52441 tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc
    52501 ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa
    52561 aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag
    52621 gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa
    52681 cgaatttgca ccggaactgg agctgcctac cgttcatatc gacaatctga ccgccgcatt
    52741 tgatgcagta aattatttat atgagcaagg gcataatcgg attggctgta tagccggtcc
    52801 cgaagagatg ccgctgtgtc actaccgcct gcaaggctat gtccaggcgc tgcgtcgctg
    52861 cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag
    52921 caaagcgatg cagcagttgc ttgatcttcc acaaccgcct actgctgtct tctgccatag
    52981 cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga
    53041 agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct
    53101 gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga
    53161 tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat
    53221 ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa
    53281 aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag
    53341 tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca
    53401 cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg
    53461 ccgccgttct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag
    53521 agtccgagac actgcaaagc cagaaagtga ccggaaacgg actaccacca aaaccagaag
    53581 aacgctggcg ctacatcaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcctacag
    53641 aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgccagc
    53701 ttcttgagca aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt
    53761 ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca gcgccagcgt caggcgcagc
    53821 aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc
    53881 agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg
    53941 cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt
    54001 cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg
    54061 agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg
    54121 cagagacggt acgtgctcag ctggcgttcg aaggctttga ctcaaaaatt accaccaaca
    54181 atggctggaa tcgtgtggtg attggcccgg tgaaaggcaa agagaacgca gacagcaccc
    54241 tcaatcgctt gaagatagcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa
    54301 accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg
    54361 tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa
    54421 cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg
    54481 caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg
    54541 tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg
    54601 ccatctggtc aaagccgccg ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg
    54661 caaacttgaa gcattgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa
    54721 cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc
    54781 ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg aaattgctga
    54841 aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga
    54901 agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt
    54961 cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttccg tggcgattgc
    55021 tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc
    55081 gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgcct
    55141 ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg
    55201 ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat
    55261 ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg
    55321 tattctcgac gtactgatcc caccggctaa aaataactgg ggacagaccg aacagcagca
    55381 ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga
    55441 caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc
    55501 gggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg gcggtcagaa
    55561 acaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc
    55621 ggcgaaactg gtgaacccgg aagagctgaa gcaagatgct atcgacgctg ttgagcagca
    55681 cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc
    55741 ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcactgt
    55801 ttccaccaaa catgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt
    55861 ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcgtctgc caatccgcgt
    55921 tgagctgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc
    55981 tatcaccgtg cagtacaaag cactgatggc aactgaaggc gtaaatatcg agtttaccga
    56041 ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat
    56101 cggtgctcgt cgtttacata ccgttctgga gcgtttaatg gaagagattt cctacgacgc
    56161 cagcgattta agcggtcaaa ctatcactat tgacgcagat tatgtgagca aacatctgga
    56221 tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt
    56281 catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca
    56341 acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc
    56401 ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact tcgatccact
    56461 ggtcgccctg ctggcattga ttaccgccgg gctattacag atcctttcta acctcgccaa
    56521 tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg
    56581 catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgttgt
    56641 gctcatctgt ctttccgggc tggcactggt tgcagtggcg tgccatacgc tggccgattt
    56701 tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg
    56761 caatcgtcct tatggttata tcggtttggg tgatatttcc gtactggttt tctttggctg
    56821 gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct
    56881 tccggcaacc gcatgcggcc tgctggcaac ggcagtactg aacattaata acctgcgtga
    56941 tatcaatagc gaccgcgaaa acggcaaaaa cacgctggtg gtgcgcttag gtgcagtaaa
    57001 cgcgcgtcgt tatcatgcct gcctgctgat gggctcactg gtgtgtctgg cgctgtttaa
    57061 tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt
    57121 gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac cgatgctgga
    57181 acgcactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag
    57241 ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc caacagccta
    57301 catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat
    57361 acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc
    57421 aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac
    57481 aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat
    57541 ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa
    57601 aatgaatggg aaggtctggt catttacggc gcggtgcgtc aggtagatga cctggaagag
    57661 ctggatatcg gcatccaggc aatggccgca attccggttg gtgccgctgg cgaaggcatt
    57721 ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg cgaccatctt
    57781 tacgccgaca ataccgggat tattctttca gaagatccgc tggatattga atgataaaaa
    57841 aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac
    57901 gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc
    57961 ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg
    58021 atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat
    58081 ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc
    58141 aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa
    58201 atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta gttagctccg
    58261 attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac ggtttgccac
    58321 acttttcatc cttctcctgg agacataatc cacaccaatc gaaaatgtta ataaatttgt
    58381 tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa tggcgcgata
    58441 acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt ttttgctcgt
    58501 taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt tcacgcatac
    58561 aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg aaaggccagt
    58621 gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt tgcgttgcag
    58681 cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt tggggactgg
    58741 gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt aaccccgctg
    58801 ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatc ccttttatcg
    58861 tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt tactacaatt
    58921 tatttttcga cttcgagcag actcatcata ttgttcgcgg cagtgttgaa agtgttgatc
    58981 tggctggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag gctttcgcag
    59041 ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg gacgatggca
    59101 acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt gcggtcattg
    59161 gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac ttcggtccga
    59221 aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc agagacattc
    59281 cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt gcatttgcct
    59341 accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa gaaaaggaga
    59401 ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca attaaacatg
    59461 actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg cgcggtcgta
    59521 atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca aatctaccca
    59581 aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag ctccacgctg
    59641 gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat cggtattacg
    59701 aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc
    59761 attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg tgacggttta
    59821 gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttctc tggcaccaaa
    59881 gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg tggtgaattg
    59941 ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg tgtccatgtg
    60001 accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct ggactgggac
    60061 gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt gcgtcgttct
    60121 tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat tccaatctcc
    60181 gggatcgccg gtgaccagca ggcggcgctg tttggtcagt tgtgcgtgaa agaaggaatg
    60241 gcgaagaaca cctatggcac tggctgcttt atgctgatga acactggcga gaaagcggtg
    60301 aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga agtaaactat
    60361 gcgctggaag gtgcggtgtt tatggcgggc gcgtccattc agtggctgcg cgatgagatg
    60421 aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca aaacaccaat
    60481 ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc catactggga tccgtatgca
    60541 cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat acgcgcgacg
    60601 ctggagtcta ttgcttatca gacgcgtgac gtgctggaag caatgcaggc cgactccggt
    60661 attcgtctgc acgcgctgcg cgtggacggc ggtgcggtgg cgaacaattt cctgatgcag
    60721 ttccagtccg atattctcgg tacccgcgtt gagcgcccgg aagtgcgcga agtcaccgct
    60781 ttgggtgcgg cctatcttgc tggtctggcg gttggcttct ggcagaatct tgacgagctg
    60841 caagagaaag cggtgattga acgcgagttc cgtccaggca tcgaaaccac tgagcgtaat
    60901 taccgttacg caggctggaa aaaagcggtc aaacgcgcga tggcatggga agaacacgac
    60961 gagtaataat gattgcctga tgcgacgctt gcgcatctta tcaggcctac gagtcggata
    61021 cacatccggc atcacgcaga ctctgaaacc gtgcgggggc aaccccgcac acatcaataa
    61081 tcccgccctt cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga
    61141 gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc agcggcgctg gctggctaca
    61201 aatggttagg acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta
    61261 ttatgctcaa ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg
    61321 aagcaccgat gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata
    61381 ttgctgttga tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg
    61441 cggtgctggc agtaggcgat aaaggctgtt tcctcaacgc gccggatatg tatatggaga
    61501 agctgatcgt cggaccggga gccaaaggca ccattgatct gaacctgccg ctggcggata
    61561 acctgcgcaa tgttgcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc
    61621 tggcaaaacc gcgccacgat gccgttatcg cagaaatgca gcaactcggc gtgcgcgtat
    61681 ttgctattcc ggacggcgac gttgcagcct caattctcac ctgtatgcca gacagcgaag
    61741 ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc
    61801 gcgcattaga tggtgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca
    61861 acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg
    61921 aagccggtaa agtattgcgt ttgggcgata tggcgcgcag cgacaacgtc atcttttctg
    61981 ccaccggcat caccaaaggt gatctgctgg aaggtattag ccgcaaaggc aatatcgcca
    62041 ccaccgaaac gctgctgatc cgtggtaaat cacgcaccat tcgtcgcatt cagtccatcc
    62101 actatctgga tcgtaaagac ccggaaatgc aggtgcacat cctctgattg atttgatcga
    62161 ttgagccttc cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa
    62221 gtcaatcaaa acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc
    62281 agaactggac cgacgccctg tttagtctca ccgttcacgc tcccgtgctt ccgtttaccg
    62341 ccgggcaatt caccaaactt ggccttgaaa ttgaaggcga acgcgttcag cgcgcctact
    62401 cctatgttaa ctcgcccgat aatccggatc tggagtttta cctggtcacc gtccccgatg
    62461 gcaaattaag tccacgactg gcggcactga aaccgggcga tgaagtgcag gtggttagcg
    62521 aagccgctgg cttctttgtg ctggatgaag taccagattg cgaaacgcta tggatgctgg
    62581 caaccggtac agcgattggc ccttatttat cgattctgca attgggcaaa gatttagaac
    62641 gcttcaaaaa catggtgctg gttcacgccg cacgatatgc cgccgactta agctatctac
    62701 ctctgatgca ggaactggaa aaacgctacg aggggaaact gcgcattcag acggtggtca
    62761 gtcgggaaac ggctgcgggt tcgctcactg ggcgattacc ggcgttaatt gaaagtggtg
    62821 aactggaaag cgcggttggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg
    62881 gcaatccaca gatggtgcgc gatacacaac agttgctgaa ggagacccgg cagatgacga
    62941 aacatttacg tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt
    63001 gtcgataaac ggcacgatca agagatccgc actcaactta ttgatcatcc cgcgatatgc
    63061 cggcatcaaa cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc
    63121 tttttgcata atttccagca gcgtttccgg catttctccg cgttcaatac gcagttttgt
    63181 cttcggccat tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa
    63241 caattgaaga atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc
    63301 atcatcaata tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt
    63361 attcaccagt aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc
    63421 catgtttaac tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa
    63481 ataatcatct cggcctggca tgagaaggtg aaggccgcat caagctcaac ttgcccctcc
    63541 agatccttaa tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca
    63601 agctctttca gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa
    63661 aacttactgg tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta
    63721 gtactgcatt tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg
    63781 attacgactt aagaataatg cgccgttaaa ggaagaacaa acaagtaatg atcttatgga
    63841 ttaccatttt ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggcattgt
    63901 cacttgcatc gtggaaataa caatcccgga aaaaaatagc gataagagat atattaaagg
    63961 ttaattactt gatttattgt cggctttata cttcacatcc tgggtatctt taccatattt
    64021 attttcgcct tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa
    64081 tgtcgggaca aaacgcccca ccgcccattg ccagacaccc ggtaaaattg cccagttacc
    64141 cgccagcagc atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc
    64201 atgcaagcgc ttaacggtta ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc
    64261 cgcggtctga atatcgagta aatttttacc cgccagtgag aaaagcacca gcatgcctgc
    64321 gaaccacagg cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa
    64381 taaccattgc tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc
    64441 ttttgacaag ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc
    64501 aatgaagcca gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac
    64561 ggcgcatgca caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc
    64621 tactttcgat ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct
    64681 gccactgaac gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc
    64741 agcaagtaaa attaacgaga acttgtatgc ttctactgcg ctggagcgcg gtaccttaaa
    64801 aatcaaaagc attcaaatga cctggctacc catccagggg ccagagcaaa aagccgcgaa
    64861 agcgaaagct caggcataca tggcagcggt gatccgcaca ctcaccccat taatgaccaa
    64921 aactcaaagc cagaaaaaac tgcaggcgct actaacggcg gggaaaaaca aacgttatta
    64981 caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac
    65041 cttcgctgtt gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg
    65101 acaaaaagca aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct
    65161 gcggggcggc catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct
    65221 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggttcacga gctggtatct
    65281 aacctgcgta aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa
    65341 atgtacatcg atatggcgaa gcgtgaagct gaaggcagcc acatcatgct gggtgcgcaa
    65401 aacgtggacc tgaacctgtc cggcgcattc accggtgaaa cctctgctgc tatgctgaaa
    65461 gacatcggcg cacagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag
    65521 tctgacgaac tgatcgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt
    65581 ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggca aaactgaaga agtttgtgca
    65641 cgtcagatcg acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc
    65701 gcttacgaac ccgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag
    65761 gctgttcaca aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa
    65821 gtgatcattc agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcaa
    65881 ccagacatcg acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg
    65941 atcgttaaag ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata
    66001 tccggcactt actttcctta actcctcgcc ttaacgcaaa atctcacact gatgatcctg
    66061 gatttcctcg gctgaagcac ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac
    66121 aaatgagtta tcactctgcc gcaccatcgc cagcccgtag cttcccatat gttcccgcgc
    66181 ctcaggcact tcttctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt
    66241 tacccgacgc gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct
    66301 gctgatgttc gccagattgt tatccagctg ttcgcgcaca tcaggacgaa tacaagagat
    66361 atgaatatga aaatggtttt gcgtacgccc ggtgcgagag ttgatcgcca aagaaaccgc
    66421 gcgatcggga accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa
    66481 ccaaaagaag ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg
    66541 atacgttggc atcaacagat attgcagtgg gccattaaga tcttttaaaa ccacgtatcc
    66601 ggcattgggt ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa
    66661 acattcctca aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta
    66721 accaataccg gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt
    66781 tttcatcttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat
    66841 aaaaaaccca gtcgcgagaa accaccgggt ttgataatta tcctggagaa atcagcgttt
    66901 gctaatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc
    66961 gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt cgtacttttt
    67021 cgccacctca gcatcgcgcg gacggtagta gtttttcgcg gcaatttcct gaccttctgg
    67081 cgagtagaga tatttcaggt aggcttccgc cacctctttg gtgccttttt tctcgaccac
    67141 tttatcgacc accgacacgg ttggctctgc gaggatagac tcactcggcg tgacaatttc
    67201 gaatttatct ttccccagtt cattcgctgc cagcagagct tcgttttccc aggcaatcag
    67261 tacatcgcca atcccgcgct cgacaaaggt attagtggag ccgcgcgcgc cagaatccag
    67321 aacttcgacg tttttataca gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt
    67381 gttgtgatgc agcgcgtagc cccaggctgc caggtagttc cagcgcgcgc caccagagct
    67441 tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc agtcatggat
    67501 ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg gaagtgtacg gtgcggagtt
    67561 atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa tcgcgtccac
    67621 gtcataggcc agagccagcg tgacaacatc agcttcaata ccgttgatta ccgacgtcgc
    67681 ttgtttgcct gaaccaccgt gggactgacg gatcaccacg ttatcgccag tttgctgttt
    67741 ccagtgggcg ctgaatacct tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga
    67801 aacgttaaga agctgaatat cctttgccat aacgctggtt gccgccagca aaaatgttaa
    67861 ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg
    67921 tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac ttgcgggtat
    67981 atgtaggggg attaaaaagg cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt
    68041 gtccatgccg gatgcggcgc gaacgcctta tccggcctac aaaagttcgc aaattcaata
    68101 aattgcagaa ttcatgtagg cctgataagc gaagcgcatc aggcattttt gcttctgtca
    68161 tcggtttcag gctaaaggaa tctgcctttt tccgaaatca ttaatacagt tttttcgcgc
    68221 agtccagcca gtcgcctttg aacggacgct tcatgttttc gatagcgtcg atgatgtcgt
    68281 ggtgaaccag ctgttcgttc tggataccta cgcaacgacc accgtaacct gccagcagca
    68341 gctcgatagc gtaagcgccc atacgggaag ccagaatacg gtcgtaaggc accggagaac
    68401 caccgcgctg gatgtggccc agcacagttg cgcgggtttc acgaccggtt tctttctcga
    68461 tgaaatgcgc cagttcgtca acatcacaca tatgttcggt aatcgccact atcgcgtgtt
    68521 ttttaccttt cgcgataccc gctttgattt cgtttaccag gtcttcacgg ctgaattcaa
    68581 cttccggaac cacaacgaat tcacagccac cggcaatagc cgcagccaaa gtcagatcgc
    68641 cacaataacg gcccatcact tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac
    68701 gcagacggtc gatcgcttct acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt
    68761 cagtgccttt gatgtcgttg tcgatagtgc ccggcagacc gatgcacggg aagcccattt
    68821 cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga
    68881 taccacgttt tttcaggttt tcgatagcca cggcgcggat gttctcgtcg cggaattccg
    68941 ggaaacgcgc agaaccgagg aacgtaccac cacggttgat catgtcagaa acgctgtaac
    69001 ggtctagctg taccatacgg tcttcataca gacccagata gccgtcataa atgcccatta
    69061 cttccagacc ttctgtcagc gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg
    69121 gcgcatcacc gccgcttgtc aacacaccga ttttcttaat catgactacc tctgaacttt
    69181 ggaatgcaaa atgaaatctg ttgccggaag tcttcttgca catcgaagtg atccaacgaa
    69241 tgtgcaaata gtataacaat cacttcctgc tgaattgatt caggtcaggc caaatggcgg
    69301 tattttatac acaaaatgcg ggtctggctc tcttttatac tgattatgaa agcatagacc
    69361 gtttaccctc cctgggtacg acggaacagg ggtcctgatg gataatcaca tccgatcccg
    69421 gaaaacgccg taaaatagcc tgctctacct gatccgccac catatgtgcc tgaaccaaag
    69481 gcagagagtc ttccatttcc aaatgaatct gaataaagcg ggtcggccct gactgccgcg
    69541 tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt cacgatatca ataatttctt
    69601 gccgttcttc atcaggcaat gcgcgatcca gtaatgactg taccgcctca tatcccatgc
    69661 gtaacgcgct atataaaata tagatgccga ttcccaatgc aaacagagca tcggcgcgat
    69721 gccagccgta ccaggacaac cccagcgcca gcagaattgc accgttcatc ataacatcag
    69781 actggtaatg tagcatatca gcccgcaccg cctggctttg cgtccggcgc accacccaac
    69841 gctgaaacga gacaaggata atcgtacaaa ttagcgccac aattgtcacg ataaccccga
    69901 cgcctggatc tgtcatcggt gttggagata tcagatgttg aatacccgtc aaaaacagga
    69961 atagtgctga accggagata aacatacttt gcgccagcgc cgcgagggac tcagctttac
    70021 cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga atatcgcacc accagtaaat
    70081 tcgtcaacga cgcgccgata tccaccagcg aatccaccag cgcggcgaga atactcaccg
    70141 acccggtata ccaccatgca aaaattttaa tcagcaatag cagtgaagcc atcgccgttg
    70201 cagcaatcgc cgcccgactg accagccgtc cataagattg attcataaat actcccgcta
    70261 tcaactgacg ctagtataac ggaagcaaat catctgcaat gcattaagca gcaggcaaat
    70321 tgaggataaa aaaaaccccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag
    70381 gaaaacaggg tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa
    70441 cttttttgcc attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcattt
    70501 aaaaccgctt gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc
    70561 tcaacctgac gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt
    70621 tcatcaaaat tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca
    70681 ggaggctgtt cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg
    70741 gttaaactta tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc
    70801 ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc
    70861 gttgaggcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc
    70921 gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa
    70981 caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta
    71041 aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga
    71101 gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga
    71161 tcttctggac gacagcattg atttactttt gcttgatgta atgatgccga agaaaaatgg
    71221 tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc
    71281 gcgcggcagt gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc
    71341 gaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca
    71401 ctggagcgaa caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt
    71461 aagtctcaat cctggtcgcc aggaagccac cttcgacggg caaacactgg agttaaccgg
    71521 tactgagttt accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga
    71581 gcatttaagc caggaagtgc tgggcaagcg cctgacgcct ttcgaccgcg ctatcgatat
    71641 gcatatttcc aacctgcgtc gtaaactgcc ggatcgtaag gatggtcacc cgtggtttaa
    71701 aaccctgcgt ggtcgtggct atctgatggt ttctgcttca tgataggcag cttaaccgcg
    71761 cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta
    71821 cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg tcagggtctg
    71881 atgattgagc agcatgttga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg
    71941 tggcggcgtc tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg
    72001 gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac
    72061 tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa
    72121 ctggtcggtc cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt
    72181 ccggccagca gttctcaatc cgatttcata aacttactgt ttgaccgccc gctattactg
    72241 ctgattgtca ccatgctggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca
    72301 aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccaaggaaa cttacgccag
    72361 catccggaac tggaagcggg gccacaggaa ttccttgccg ccggtgccag ttttaaccag
    72421 atggtcaccg cgctggagcg catgatgacc tctcagcaac gtctactttc tgatatctct
    72481 cacgaactgc gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt
    72541 agcggtgaaa gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg
    72601 atcaacgatc tgttggtgat gtcacgtaat cagcagaaaa atgcgctggt tagcgagacc
    72661 atcaaagcca accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcag
    72721 atgggcaagt cgttgacggt taacttcccg cctgggccgt ggccgctgta cggcaacccg
    72781 aacgccctgg agagtgcgct ggaaaacatt gttcgtaatg ccctgcgtta ttcccatacg
    72841 aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat
    72901 ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcgtaccgat
    72961 gaagcacgcg atcgtgaatc tggcggtaca ggtttggggc tggcgattgt agaaaccgcc
    73021 attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg
    73081 gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc
    73141 gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc
    73201 aggcctacac tccggggatt attttcccca caatcggcgg gaaaaatctt cgatcttgcc
    73261 gctcagtcgg cgcttttgca tcgttcgcgt ccagcttttc gatacccctg cggcggagag
    73321 taaacggtga tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg
    73381 cacggtcaca tcgctgacac gggaaaccaa ctccagtggc gcatcagcag ataccaaccc
    73441 tggtgcaatt acgctataca accaccccac tttacccgtg ttttgcatca actgcgctat
    73501 atcgctgata tcaaaatggt aattgagttt atagcaaggt gagcgtggct ggctgacctg
    73561 aattaatgcc tctccccagc ggaaaatatc gccaatataa acattacttt ccgtcagacc
    73621 gtcggttgag agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa
    73681 ttcccgcgcc cagtaaagat aatgctcacg aggataatga cacagcgctc tgtctggccc
    73741 gccgtgaacc tttttctccg cctgctcgtc accttccagc cccaggtccg tcagcatcaa
    73801 ctcaccatca acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat
    73861 cttgcctgtg tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc
    73921 cattagacca tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg
    73981 aactcaatag caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg
    74041 tttatttcac cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc
    74101 agatagaggt aagaattggc accagaatcg aagtcacaat gaccgccgtc gcgaccagcg
    74161 aagttgctgc cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg
    74221 tcgctaccgc ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc
    74281 caatcaattt atcagcgata atcaacggaa taccggtcac gataattact gccacaccca
    74341 acaggatccc cagcaaacct gtctgggcaa ttaccgtcaa atcaatggta ttacccagtg
    74401 cgaaggcaaa gaatggaatc agcgtctgca ccgctttgct gaaaaattct cgtaattcag
    74461 ggtcaaggtt cccaagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat
    74521 gcggttcaaa cgaggcaatc ccggcagtgc ccagaataat catcgtcatg agcggcccgg
    74581 actccaatga catcaacaca aatgccccag cttcttcttt tgtgccgtac tgctgcatga
    74641 tggaagcgta aagtccgccg ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa
    74701 gtccggcaaa gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg
    74761 caaccaccca cgcgacggca atttttgtta ccaccagcgt accggattta cgcaataccg
    74821 ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg
    74881 gcaccgtacc ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcctccggcg
    74941 agaaggtgtg gcacagtgcg ccaaggaata acgggacgag catcatcccc cccgggattt
    75001 tctcaatcga gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat
    75061 gcgtcattcc tcaatggagc atcagtgttg atggaaacga agatagacat tcatttaatc
    75121 aataaaagtg atcaaaacca catcaattga aacactgttt tatttttatc ggatcattgt
    75181 ttttattttg aaattgatca caaaaaaacc accgccgttg gcgatggttc attttttaag
    75241 ctgatatgcg gcgtaaacgc ctcattgcag caggcggcaa atgattattt tttcgccgcg
    75301 aaacgtgccg ctgcttcgtc ccagttcacc acgttccaga actctttgat gtagtcagga
    75361 cggcggttct ggaatttcag gtagtaagca tgttcccaca catccaggcc cagaatcggg
    75421 aagccggaag cgccagaaat agcttcaccc atcagcgggg aatcctggtt agcagtagaa
    75481 accaccgcca gtttatcgcc tttcagcacc agccatgccc agccggaacc aaagcgggaa
    75541 gctgccgctt tctcaaattc tgctttgaag ttatcaacgg agccgaagtc acgttcgata
    75601 gccgctttca ggtcaccctg cagggtggtg ccttttttca gacctttcca gaacaggctg
    75661 tggttagcgt gaccgccagc gttgttgcgc agtacggttt tcttgtctgc tggcagctgg
    75721 tccagtttgg tgatcagctc ttcaaccggc aggttggcaa attctggcag gctttccagc
    75781 gccgcgttgg cgttgttgac gtaggtctga tggtgtttgg tgtggtggat ttccatggtc
    75841 tgcttatcga agtgcggttc cagggcatcg taagcatacg gcagggatgg cagggtataa
    75901 ctcatattca tctccagtgt tgtcgggcgg ccgattgtta atgccgcgta agcagttggt
    75961 tcattatagt taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg
    76021 tatggttttg caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt
    76081 gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca
    76141 ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag gataagaaga tgagtaacgc
    76201 gattacgatg gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc
    76261 tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg tggtcagttg gtgggattgt
    76321 ttcgtggatt attctgccgt gggccatcag cgccctgtta ctaccgaatt tctgggcgta
    76381 ttacagctcg tttagtctct ctacgctgct gcctgttttt ctgttcggcg caatgtgggg
    76441 gattggtaat atcaactacg gcctgaccat gcgttatctc ggcatgtcga tggggattgg
    76501 tatcgccatt ggtatcacgt tgattgttgg tacgctgatg acgccaatta tcaacggcaa
    76561 tttcgatgtg ttgatcaata ccgaaggtgg acgcatgaca ttgctcggcg ttctggtggc
    76621 gctgattggt gtagggattg tgactcgcgc cgggcagttg aaagagcgca agatgggcat
    76681 taaagccgaa gagttcaacc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt
    76741 ctctgccgga atgtcctttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc
    76801 actgggcgtc gatccactgt atgtcgctct gccaagctat gttgtcatca tgggcggcgg
    76861 cgcgatcatt aacctcggtt tctgcttcat tcgtctggca aaagtgaagg atttgtcgct
    76921 aaaagccgac ttctcgctgg caaaaccgct gatcattcac aatgtgttac tctcagcact
    76981 gggcgggttg atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc
    77041 ggcgcagtat gactacatca gttggatgct gcatatgagc ttctatgtat tgtgcggcgg
    77101 tatcgtcggg ttggtgctga aagagtggaa caatgcaggt cgccgtccgg taacggtgtt
    77161 gagcctcggt tgtgtggtga ttattgtcgc cgccaacatc gtcggcatgg gcatggcgaa
    77221 ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt
    77281 aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggttgaaa tatcactgat
    77341 taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct gccgcagata
    77401 ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca
    77461 cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctgg ccgccagccg
    77521 ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta
    77581 acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactctgcca tttcgttagc
    77641 aaacggcaca tgctgactac tttcatgctc aagctgaccg ataatctgcc gcgcctgcgc
    77701 catccccacg ctacctaagc gccagtgtgg ttgccctgcg ctggcactaa atcccggaat
    77761 cgccccctgc cagtcaagat tcagcttcag ccgctccggg caataaataa tattctgcaa
    77821 aaccagatcg ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc
    77881 gccacgggta atgcgataag ggcgatcgtt gagtacatgc agaccattac cgcgccagac
    77941 aattaccagc tcacaaaaat catgtgtatg ttcagcaaag acatcttgcg gataacggtc
    78001 agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg
    78061 atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac
    78121 aaatacgttg agaagattcg cgttattgca gaaaaccatc ccgtccctga cgaatatcac
    78181 gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc
    78241 cgcagcgata ggcgatgtca gtaacgctgg cctcgctgtg gcgtagcaga tatcgggctt
    78301 tcatcagtcg caggcggttc aggtaacgct gaggcgtcag ccccgtttgc tgcttaagct
    78361 ggcgatgcag cgtacgcaat gaaagagaaa attgatccgc cacggcatcc caattcacct
    78421 catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgac gcgctgtttt
    78481 ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata aataagatct
    78541 cgcgactggc ggtcgagggt aaatcatttt cctcttcctg ctgttccatc tgtgcaacca
    78601 gctgtcgcac ctgctgcaac acgctgtggt taacgcgcca gtgagacgga tactgcccat
    78661 cctgctcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc
    78721 gatacagcac attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgat
    78781 cgcgtacgaa acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa
    78841 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat
    78901 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca
    78961 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca
    79021 cgaggtcagg ttcttacctt aaattttcga cgggaaacca cgtaaaaaac gtcgattttt
    79081 caagatacag cgtgaatttt caggaaatgc ggtgagcatc acaccaccac aattcagcga
    79141 attgtgaaca tcatcacgtt catctttccc tggttgccaa tgacccattt tcctgtcagt
    79201 aacgagaagg tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat
    79261 cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tggacgcgtg
    79321 atgctggcgc gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt
    79381 aacaatgggc tgcatagcca gaacggttat gtcacctggg atgtggatag cctggaaagt
    79441 gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg
    79501 attgatacct ggggcgtgga ctttgtgctg cttgaccaac agggtcagcg cgtgggcctg
    79561 cctgttgctt atcgcgatag ccgcacccat ggtctgatgg cgcaggcaca acggcaactc
    79621 ggcaaatgcg atatttatca acgtagcggc atccagtttt tgcccttcaa tacgctttat
    79681 cagttgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg
    79741 ctgatgccgg attacttcag ttatcgtctg accggcaaga tgaactggga atataccaac
    79801 gccacgacca cgcaactggt caatatcaat agcgacgact gggacgagtc gctactggcg
    79861 tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcattggg
    79921 cactggattt gcccgcagag taatgagatt ccggtggtcg ccgttgccag ccatgatacc
    79981 gccagcgcgg ttatcgcctc gccgttaaac agttcacgcg ccgcttatct ctcttctggc
    80041 acctggtcat tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca
    80101 gccaacatca ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg
    80161 ggcttatggc tgcttcagcg ggtgcttcag gagcggcaaa tcaacgatct tccggcgctt
    80221 atcgccgcga cacaggcact tccggcttgc cgcttcacca tcaatcccaa tgacgatcgc
    80281 tttattaatc ctgacgagat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa
    80341 ccaatcccgg aaagtgatgc tgaactggcg cgctgtattt tcgacagtct ggcgctgctg
    80401 tatgccgatg tgttgcatga gctggcgcaa ctgcgcggtg aagatttctc acaactgcat
    80461 attgtcggcg gtggctgcca gaacgcgctg ctcaaccagt tatgtgctga tgcctgcggt
    80521 attcgggtga tcgccgggcc tgttgaagcc tcgacgctcg gcaatatcgg catccagtta
    80581 atgacgctgg atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg
    80641 aatctgacca cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagatt
    80701 cactctacac gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg
    80761 agctggcgaa acagcgtttc gcggcagtag ggattgatgt cgaggaggcg ctgcgccaac
    80821 ttgatcgttt acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa
    80881 acccggaagg ttcgctgacc ggtgggattc aggctactgg taattatccg ggcaaagcgc
    80941 gtaatgccag cgagctacgt gccgatctgg aacaagctat gcggctgatt ccggggccga
    81001 aacggcttaa tttacatgcc atctatctgg aatcagatac gccagtcgcg cgcgaccaaa
    81061 tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa aaccaatcag ctcggtctgg
    81121 attttaaccc ctcctgtttt tcgcatccgc taagcgccga tggctttacg ctttcccatg
    81181 ccgacgacag tattcgccag ttctggattg atcactgcaa ggccagccgc cgtgtttcag
    81241 cctattttgg cgagcaactc ggcacaccgt cggtgatgaa catctggatc ccggatggca
    81301 tgaaagatat caccgttgac cgtctcgccc cgcgccagcg tctgctggca gcactggatg
    81361 aggtgatcag cgagaagctg gatccggcgc atcatatcga tgccgttgag agcaaattgt
    81421 ttggcattgg cgcggagagc tacacggttg gctccaatga gttttacctg gggtatgcca
    81481 ccagccgcca gacggcgctg tgcctggacg ccgggcattt ccacccgact gaagtgattt
    81541 ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc
    81601 cggttcgctg ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcgattgcca
    81661 gtgagattgt tcgtcacgat ctgttcgacc gggtgcatat cggcctcgac ttcttcgatg
    81721 cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc
    81781 tacgtgcgtt actggaacct accgctgaac tacgcaagct ggaatcggcg ggcgattaca
    81841 ctgcgcgtct ggcgctactg gaagagcaga aatcgttgcc gtggcaggcg gtatgggaaa
    81901 tgtattgtca gcgtcacgat acgccagcgg gtagcgaatg gctggagaac gtgcgggctt
    81961 atgagaaaga aattttgagt cgccgcgggt aaacactgcc ggatgcggca caagtgcctt
    82021 atccggccta cgggtcggca atagttgtag gcctgataag acgcgacagc gtcgcatcag
    82081 gcattgattg ccggatgcgg cacgagtgcc ttatccggcc tacagatcgt atatccggca
    82141 acaggcacgg acgtccaatt aacaggaaca ccgaacatgc aaaacattac tcagtcctgg
    82201 tttgtccagg gaatgattaa agccaccacc gacgcctggc tgaaaggctg ggacgagcgc
    82261 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcaccata tcacgacaat
    82321 tttcaccaac aaccgcgcta tatcccgctc agccagccca tgcctttact ggcaaataca
    82381 ccgtttattg tcaccggctc aggcaaattc ttccgtaacg tccagcttga tcctgcggct
    82441 aacttaggca tcgtaaaagt cgacagcgac ggcgcgggct accacattct ttgggggtta
    82501 accaacaaag ccgtccccac ttccgaactt ccggctcact ttctctccca ctgcgagcgc
    82561 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccatgccac caacctgatc
    82621 gcccttacct acgtactgga aaacgacacc gcggtcttca ctcgccaact gtgggaaggc
    82681 agcaccgaat gtctggtagt gttcccgaat ggcgtcggca ttttgccgtg gatggtgccc
    82741 ggcaccgacg aaatcggcca ggcgaccgca caggaaatgc aaaaacattc gctggtgttg
    82801 tggcccttcc acggcgtctt cggcagcggt ccgacactgg atgaaacatt cggtttaatc
    82861 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    82921 cagaccatca gtcgtgaaga gctgatagcg ctcggccagc gtttcggcgt tacaccactc
    82981 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggtt
    83041 aaccccgacg cccacgaaga gtatcagcgt cgtcataatc caatctggcc ggaactggaa
    83101 gcagtgttga aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat
    83161 ctgctgtttg ccacggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    83221 gatgtttgcc aacgttggtg gaaatatatg accgacgtta tgccaactaa cccggataac
    83281 agcccagtca gtagcgagct gcaagaagtg ttttacctgc cttaatgagt tgcggtgccg
    83341 gaaaatatcc agcaccgcga cttcatcaga agaagtattt aaagcgaacg cgtgcaccgt
    83401 aacggtcatc agtatcatca ccgtgttttt tatcaccgtc gctatccaga atggagtaat
    83461 acgtcccgag gtaaatatta aagttctcca tatccatcac attagcgaac tgataagacg
    83521 catgaatggt gtgaatggtg tacgtccctt catcatcaat ccagcagtcg ttatcacaaa
    83581 ctacaccgct gaattcatca atcttattgt gcgcgtagat ataacccagc tcgaaacgtt
    83641 tccacagcgc gttaatcccg gcagtgaaat ctttctcatt attagcatct aaataggcgg
    83701 tattaagatt aaccacgaca ccattttccg gatccgtttt caggccattc caggtcatgc
    83761 tcataccata gccggtacga tcggactgat cgacaaaatt acccttgcta tcggtataac
    83821 cataagcatt atttaccacg ttcgcttcca tcgctgcgga aacggtgaat tcttccgtcg
    83881 gcgaccaggc aattaccgga cgcagataag caacattttt ctgctgttcc atatcgcgtc
    83941 catgataatt accgtcgtta tataaagatg ttccgtcttc cagtaacgtg tttaattcaa
    84001 aataccagtt atcgagttgt ttgctgacga ggaaattacc gccagcgtta gaacgcccgc
    84061 ggccctcttt catcatatag atataaccgc tgccatcgtc ataaagatcg ttcgcagtat
    84121 taccggaatg ttcaacaaag gtatcctgat tcagcgggaa catatcgtag gcttcaaaac
    84181 ggcctacttt gaccttccag tcgttttctt tgccaaagaa gaataccgca tcatcgatat
    84241 ttactgaacc gtgcatgtcc cccagcggtt gtgcggagaa cccggcgaaa taaccgttgt
    84301 ccatctttcg cataccgtca aaacctaaca gaatacgacc attgagattc cactgttcat
    84361 tagtgggatc gctattcaca tcagcgttgg tcattgccag aaggccatga ttactttccg
    84421 catccatatt aaattctacg tcgccgtaga tttttaaatc gccataacct gaaagcaaaa
    84481 gattaggtga ggtggaattt ttctttgcag attccgacgt tgcctgtcca tttaccggcg
    84541 tattgccctg ggcagcctta atttcgcgga tctcagcggc ttgttgtgtc tgcagctgtt
    84601 taacctggct ctctgctttt gctgcccgca tttctgccgc ttccaggcgt tcttccagca
    84661 atgccatgcg ttgttccagc gttaaatgct gtgtttttgc cattccggta ctacatgcca
    84721 ataataaacc gatggctaaa gataatgtcg attttttcat tatttacgtt ccctgaaata
    84781 aaagagcccc tacgctttcc aaaaggaata cgcatataga gaaatataag gtgtgcactt
    84841 tcgaattttt aatccaactt cagcaggccg atggatagta tcccgccata taatatgagt
    84901 aaatccgctg atttcctgga acgtgaggaa tataacaata gtgccgtttt taactaaata
    84961 tcgtaaaaaa tattagatat gattcacgct gactcataag ccacaaatat aaataacgct
    85021 taaacaaatt atctgagaca tttaaacatt cacaaaatca ttaaataatg atactttatt
    85081 cacaaaattt atccttcact ctgctttata ttgtcacgac ttcgtgacat atccgcgctc
    85141 atcacaaacc cgctccgcga ttacaattga ctgaaatatt tcatcgacca tttcgttgta
    85201 ctcgcataat gctctcggga aatgagtctt cggccatcca gcttgagttt gcgtcctgaa
    85261 gttgctcttt atcccagtca gagtacggga ctttatctct gatgcggcga acgtattttt
    85321 tagcctcgag ttgatactgg cctgccgaat taataaacga gaaattcatg ccgcttttat
    85381 ccgcttcttt ggcctgcgcc accagcgttt ccagttcaga cactttcttc attgccgctt
    85441 ctgcatcaaa cttatcttgc gatatcaggt tattaatttg tttcgctgaa atcatgactg
    85501 ccagagagta gtagtggaat gttttccctt ctctttcttc aatatttttc aattccgtaa
    85561 gctggcgctt gtcattaatt tcctgaatcg ccgcatgata agattctgct acaggttcaa
    85621 aggcttccag attttttaag aatgtctggt gcagcgtttt ccctttcgca aacgcatcgt
    85681 ctttataatt ttcctggatg taatatttat ccatttcgtt aatggtatta cccaacgcta
    85741 cggcagcatc aatataactc accgccacgc catcaatagg ttgcaacgca ggcgttaacg
    85801 acactacacg cgttttgttt ataaataaaa taataaaatt cacgaaatga ataagtcctg
    85861 caatttaata tatatcgcag gacttatttt aattaattgc tcaaacgctg cggatgggta
    85921 taaaccgttg cccgacccgg tttacaaaag cccaccagcg tcaaattaca gcgttcagcc
    85981 acttctacag caagtgtggt cgcggcagac accgcaaaca aaatctctac gccgcacatc
    86041 gccgactttt gcaccatttc ataactggca cggctggaaa ccagtaccgc accttgctgc
    86101 cagctttccc cttcttgtga ccggcggcct aacagtttgt ccagcgccac atggcgaccc
    86161 acgtcttcat gcccgccgac cagttcgcca gatggcaaca tccaggcggc ggcgtgagta
    86221 caaccggtca gttgccccac tggctgaaaa tcattgagat gacgtaatgc atcatccagt
    86281 ttgttgagat caaacgtctg ggtgaacggt agcggctgca ccggttttcc gatgtcatta
    86341 agttgctcca cgccgcacac gccacatccc gtacgtcccg ccagtgcccg gcgacgctcc
    86401 ttcaacccca taaagcggcg gctggaaagc tcaatttgaa cttcaagacc attacaggaa
    86461 ggaacgacat ccatgccgaa gatatcgcgc ggactttcga ttatcccctc ggaaagcgaa
    86521 aaaccgagcg caaagtactc aaggtctttg ggcgacgcca tcatcaccac atgcgaaata
    86581 ccgttgtaga ccagcgcaac gggaacttct tccgccacct catctaaccg tgggtgttgt
    86641 aaatcatcat gccgccataa ctcaatttga cgaacacctg tgatatttgt cacattttcg
    86701 atttcttttt gctgtgtttt tttcactttc tcttaaccag ataagaacac acacaccaac
    86761 attatggtat tctgttacaa acccttcctg gatggaggga aattgagcca attctggacc
    86821 tttgcggccc cttccgcaaa gaaaaataac tcccactccc tgcacacgca gcaagcgaat
    86881 gtaaatggga cgtgacaatg tcgaaacaag gagcaatcca tgcaggtcag cagaaggcag
    86941 ttctttaaga tctgcgctgg cggtatggca ggcaccacgg cagcggcact gggttttgca
    87001 cccagcgtag cactcgcgga aacccggcag tataaactgc tgcgcacccg cgaaacccgt
    87061 aatacctgca cctattgttc cgtaggctgt gggctgttga tgtacagcct cggtgacgga
    87121 gcaaaaaacg ccaaagcatc tatcttccat atcgaaggtg acccggatca cccggtcaac
    87181 cgcggtgcac tttgtccgaa aggcgctggc ctggtggatt tcatccactc cgaaagccgt
    87241 ctgaagtttc cggaataccg tgcgccaggt tctgataaat ggcagcaaat cagttgggaa
    87301 gaggcgtttg atcgcatcgc caaactgatg aaagaagacc gcgatgctaa ctacattgcg
    87361 caaaacgccg aaggcgtgac cgttaaccgc tggctctcca ccgggatgct gtgtgcttcc
    87421 gcgtcgagta acgaaaccgg ctatttaacg caaaaattct cccgcgcgct gggtatgctc
    87481 gcggtcgaca accaggcgcg tgtctgacac ggaccaacgg tagcaagtct tgctccaaca
    87541 tttggtcgcg gtgcgatgac caaccactgg gtcgacatca agaacgccaa cctcgtcgtg
    87601 gtgatgggcg gtaacgccgc tgaagctcac ccggtcgggt tccgctgggc gatggaagcc
    87661 aaaattcaca atggcgcgaa gctgattgtg atcgatcctc gctttacgcg tacggcggcg
    87721 gtagctgact actatgcccc tattcgttcc ggtactgaca ttgctttcct gtcaggcgta
    87781 ttgctgtacc tgctgaacaa tgaaaaattc aaccgcgaat acaccgaagc ctataccaac
    87841 gccagcctga tcgtgcgtga ggattacggc tttgaagatg gcctgttcac cggctatgac
    87901 gcggaaaaac gtaagtacga taaatcctcc tggacttacg aactggacga aaacggcttc
    87961 gccaaacgcg atactaccct gcaacatcca cgttgcgtgt ggaacttgct gaaacagcac
    88021 gtttcccgct acacgccaga tgtggttgaa aacatctgtg gtacgccaaa agacgcattc
    88081 ctgaaagtct gcgaatacat cgctgagacc agtgctcacg ataaaactgc ctcgttcctt
    88141 tacgcgctcg gctggacgca acactccatt ggtgcgcaaa acattcgtac gatggcgatg
    88201 atccagttgc tgctcggcaa tatggggatg gcaggcggcg gcgttaacgc cctgcgcggt
    88261 cactccaata ttcaggggct gacggacctg gggctgctgt cgcagagcct gccgggttac
    88321 atgacgctgc cgagcgagaa gcagaccgat ctgcaaacct accttaccgc caacacgcca
    88381 aaaccgctgc tggaaggcca ggtaaactac tggggcaact acccgaaatt cttcgtctct
    88441 atgatgaagg ccttctttgg tgataaagcg acggcggaaa atagctgggg ctttgattgg
    88501 ttgccgaagt gggataaagt ctacgacgtc ctgcaatact tcgagatgat gaaagaaggc
    88561 aaggtcaatg gctatatctg ccagggcttt aaccctgttg cctcattccc gaacaaaaac
    88621 aaagtgatcg gttgtctgtc gaaactgaag ttcctcgtca ccatcgaccc gctgaacact
    88681 gaaacctcta acttctggca gaaccacggt gagctgaacg aagttgactc gtcgaagatc
    88741 cagaccgaag tgttccgtct gccatcgact tgcttcgcag aagagaacgg ttcaatcgtt
    88801 aactccggtc gctggttaca gtggcactgg aaaggtgcgg acgccccagg gattgcgctg
    88861 actgatggcg agatcctctc cggtatcttc ctgcgcttgc gcaagatgta tgcagaacag
    88921 ggtggcgcaa acccggacca ggtgctgaac atgacctgga actacgccat tccgcatgag
    88981 ccatcttcag aagaagtggc gatggagagc aacggtaagg cgctggccga tattaccgat
    89041 ccggcaaccg gtgcggttat cgtcaagaaa ggccaacaac ttagctcgtt cgctcaactg
    89101 cgcgatgacg gtacaacctc ctgtggctgc tggattttcg ccggtagctg gacgccggaa
    89161 ggcaaccaga tggcgcgtcg tgataacgcc gatccgtctg gcctcggtaa cacgctgggc
    89221 tgggcatggg catggccgct taaccgccgc attctgtata accgcgcctc cgcagatccg
    89281 cagggtaacc catgggatcc gaagcgtcag ttgctgaaat gggacggcac taagtggacc
    89341 ggctgggata ttccggacta cagcgcagcg cctccgggca gcggcgtagg gccgtttatc
    89401 atgcagcagg aaggcatggg acgtctgttt gccctcgata agatggcgga aggtccgttc
    89461 ccggaacact acgagccgtt tgaaacgccg ctgggaacta acccgctgca tccaaacgtt
    89521 atctcgaacc cggctgcgcg tatctttaaa gacgacgccg aagcattggg taaagccgat
    89581 aagttcccgt atgtcggaac cacctatcgt ctgaccgagc acttccacta ctggaccaaa
    89641 cacgcgctgt tgaatgcgat tttgcaacca gagcagtttg tggaaatcgg ggagtcgctg
    89701 gcgaataaac ttggcattgc ccagggcgat actgtgaaag tctcctccaa ccgtggctat
    89761 atcaaagcca aagcggtagt gaccaaacgt attcgcacgc tgaaagcaaa cggcaaagat
    89821 atcgatacca tcggtattcc gattcactgg ggctatgaag gtgttgcgaa aaaaggcttt
    89881 attgccaata cgttgacgcc attcgtcggt gatgcgaaca cgcagacgcc ggagtttaag
    89941 tccttccttg tgaatgtgga aaaggtgtaa cggagacgac atatggctta tcaatcgcaa
    90001 gatatcattc gtcgttccgc gactaacggt ctgacccccg cgcctcaggc gcgggacttc
    90061 caggaagaag tggcgaaact catcgacgtt accacctgta tcggctgtaa agcctgtcag
    90121 gtggcgtgtt cagagtggaa cgacattcgc gataccgtcg gcaataacat tggggtgtac
    90181 gacaacccca atgatttaag tgccaaatcg tggacggtga tgcgattctc ggaagtggag
    90241 cagaacgaca aactggaatg gctgatccgc aaagacggct gtatgcactg ttccgatcca
    90301 ggctgcctga aagcgtgccc ggcggaaggg gcaattattc agtatgccaa cggtatcgtc
    90361 gacttccagt ccgagcagtg catcggctgc ggttattgca ttgcgggctg tccgttcgac
    90421 attccgcgcc tcaacccgga agacaaccgc gtctacaaat gtacgttgtg cgttgaccgc
    90481 gtggtggttg ggcaagaacc ggcctgcgtg aagacctgcc caacgggcgc gattcacttc
    90541 ggtacgaaag agtcgatgaa aacgctggcg agcgagcgcg tggcggagct gaaaacccgt
    90601 ggttacgaca atgcgggttt gtacgacccg gcaggcgtcg gtggtacaca tgttatgtac
    90661 gtgctgcacc atgctgacaa gccaaatctg tatcatggct tgccggagaa cccggaaatc
    90721 agcgaaaccg tgaaattctg gaaaggcatc tggaaaccgc tcgcggctgt tggctttgcg
    90781 gctacctttg cggccagtat cttccactac gtgggtgtcg gtccgaaccg tgcggatgag
    90841 gaagagaata atctgcacga agagaaagac gaggagcgca aatgaaacga cgtgacacca
    90901 tcgtgcgcta caccgcgccg gaacgtatca accactggat caccgccttc tgcttcatcc
    90961 tggcggcggt gagcgggctg ggctttttgt tcccgtcctt caactggttg atgcaaatca
    91021 tgggcacacc gcagctggcg cgcattctgc acccgtttgt cggcgtggtt atgtttgcct
    91081 cgttcatcat catgtttttc cgttactggc atcacaacct aatcaatcgg gatgatatct
    91141 tttgggcgaa gaatattcgt aagatcgtcg tcaacgagga agtaggtgac accgggcgtt
    91201 ataacttcgg tcagaaatgc gttttctggg cggcgattat tttcctggtt ctgctgctgg
    91261 tgagcggcgt gattatctgg cgtccttatt ttgcacctgc tttctcaatc ccggtgatcc
    91321 gattcgcgtt aatgctgcat tcatttgccg cagtggcgtt aattgtggtt atcatggtgc
    91381 atatctacgc cgccctttgg gtgaaaggca cgattaccgc catggtggaa ggatgggtta
    91441 ccagcgcctg ggcgaagaaa catcacccgc gctggtaccg tgaggtccgc aagacaacgg
    91501 aaaagaaagc tgaatgagta ttcgcataat cccgcaagat gagctgggtt cgagcgagaa
    91561 acgtacggcg gatatgattc cgccgttatt gttccctcgg ctcaagaatt tatacaaccg
    91621 ccgcgccgag cgtctgcgcg agctggcaga aaataatccg ctgggtgatt acctgcgctt
    91681 tgctgcgctt atcgcccacg cccaggaagt ggtgctgtac gaccatccgc tggagatgga
    91741 tctgaccacg cgcattaaag aagccagcgc acaaggcaag cccccgctgg atattcacgt
    91801 tctgccgcgt gataagcact ggcaaaagct gctgatggcg ctgattgctg agctgaaacc
    91861 tgaaatgagc ggcccggcgc tggcagtgat tgagaatctg gagaaggcat cgactcagga
    91921 gctggaagat atggccagcg cactgtttgc ctctgatttc tcgtccgtca gcagcgataa
    91981 agcgccgttt atctgggctg cgctgtcgct ctactgggcg cagatggcta atctgatccc
    92041 cggcaaagcc cgcgctgaat acggcgaaca acgtcaatat tgcccggtat gtggctctat
    92101 gccggtgtcc agcatggtgc aaattggcac cactcagggt ctgcgttacc tgcattgcaa
    92161 cctgtgtgaa accgaatggc acgtggtgcg cgtaaaatgc agcaactgcg agcagagcgg
    92221 caaactgcat tactggtcgc tggatgacga acaggccgcg attaaagccg aaagctgcga
    92281 tgactgtggc acttacctga agattcttta tcaggaaaaa gaaccgaaag ttgaagccgt
    92341 ggcagatgac ctcgcctctc tggtactgga cgcgcgaatg gagcaagaag gctacgcccg
    92401 cagttccatc aacccgttcc tgttcccggg tgaaggggag taatctctga cgttttccgg
    92461 gtggcgctgg tcgcccggat tacagctcat agggtgtgac gacacctgct attcccgcaa
    92521 aatctctcgt attttgcgag gcgttttcca gatatgatgt aacagttaca gttattataa
    92581 gttcaagtaa aacagtcttg tattaatacc agaataaggg catctggttt ttatattcaa
    92641 gaataataaa ttttttgtca ttccttatgc tccttacact gatcatcaac aactactgtc
    92701 tcaccccttt gcctgccacc ctttgcttta cgcaccggaa attgctgccg ctccagatat
    92761 tgctcaaccg cttcgcgtaa aattgcagat cgtgaaacct tacgccagcc cctaagcttg
    92821 ttcagccgtt taattgcctc ttctgataaa tgaagaaaaa ccgtattcat cgccattacc
    92881 aataccttac atatattact cattaatgta tgtgcgaaaa ttacgccgaa aaatcattta
    92941 aaaaaagcgg atttttgcat gctgttttgt ttttgcgaga cgttttccag aaataataag
    93001 aacgttgcag ttgtttcatt tgttaatcaa aaatggttgg gttatcgccc aggatgagat
    93061 atgggtgcta taaatatatt atatacaggg atgtttattt tttgattgga tctttatttg
    93121 cataatctga atggtgtagc cgaaaaatgt cagcatccta acagcacccg gatttaaggt
    93181 gaaattaatc atccatcaca gaccggtcag tactgctacc aaccggtctt aacttgctca
    93241 tcgttataat tttgttggta acagttcggg aaacaaggaa ttccttccgg aacacccccg
    93301 gtatcatccg atagcattga cgtcaatcat cggcgcgacg atgagttttt ttaatcgcat
    93361 catagggtgg caacggtcag cacgattacg ggtcatagca aaactcctga gcaggtggca
    93421 ctacgccgct ttcacaggag ttctgaatgc tggtgctttc tgtatggcat aaccatgcct
    93481 ctaacggtta agtgcttgca gcacatgttg cggatcattg gcaatcgctc gcagcaaagc
    93541 ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg tgtctacgct
    93601 tacagaaata agatcggcaa atttagattg tgacaaccct gaagcgcggc gtatttcttt
    93661 gattttcata gcatcaactt caaaagtccg cgaaggctgt cgcgttcctt caataatttc
    93721 attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca tttaccacct
    93781 cgtatttaac aaccggagca ttgctttttc ctggggagac agatcatctt taataccctt
    93841 acgataaata agcagtaatc tgatctcata atgtttaacc tgatgaaagt aaataaccct
    93901 gacaccagca cgtttcccct ttcctcctga cacccatcga acctttctta acccaccagt
    93961 ttcaggtatt acctcaccat agtcaggatt caaggccagg aaaaactgaa aacgactgaa
    94021 ttcatcgtcg gtcagcagtt tttgaacatc ttcagtaaaa atttccgttt cgatgaagag
    94081 cataattata cgccattggc ttagagtaac aatccttttt gtacgtcatt gacgtaccct
    94141 tgaaaccact agcttactga tagtgctcca gcaaaaccac gtctctgccg ggtttgtctt
    94201 tacatttgcg aggcgctttc cagatttttc acgcccgtga atttccctgt atccatccag
    94261 taaaaacggg tgtataaaac tgtacattca tacagcaaaa tgagttctac tatggcacag
    94321 gaaaaaggaa ttgcagagtt agtcgaagag tttatcgctg cgggtcgccc ctcatcgcgt
    94381 caacaaagta ttgcgcagcg cagggagggc tatatcgcca gcgctgtcct tgcgggtgag
    94441 acagaaacgc gggtagatat tcagactatc gaacttgaag cgatgacctt acgaatagtg
    94501 tcaccgctga atgccccgac attactcccg acgataattt attactacgg tggctgtttt
    94561 gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc agttagccta ttacgggcaa
    94621 tgtcgggtta ttgccgtcca gtatcggctg gcaccggaac atactttccc cgccgcccat
    94681 gatgatgcgc aacgaggtgc agaattagtc tggcaacacg ccgaacgact cggcgttgat
    94741 aaacaacgga taactcttgc cggtgacagt gcgggcggac atctggcgct ggtaactgct
    94801 ttgcgattaa agcgtgctgg cgagtggcaa ccggcacaac tcatcttaat ttatcccatg
    94861 cttgatgcga ctgcgcattt cgaaagctac atacgcaatg gtcacgatta catcatcaca
    94921 cgcgacactt tactgagcgg ctttgagatg tacctgccag gcattgaacg caggcatcct
    94981 gaagccagcc ctctttggcg cgatgatttc aatggcttgc cacctgtaca tatcattacc
    95041 gctgaatacg acccactttg cgatgaaggt gaagcgttgt atcaccgcat gacagggcaa
    95101 ggagtgcaat gcacctgcca acgctacctt ggcgttatcc acggattttt tcagttaggt
    95161 ggtatcagtg aggctgcgcg atcggcgcta cgggatgttg cctggagagc gggaaggtaa
    95221 ctccggacca gggcccagaa aaggttttca gattgaaaac cttttcagtc aagtaaaacg
    95281 cccctactct tcttcgttcc cgccctcttc atacgggcca aatggcttcg cgggcagaac
    95341 gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt ctacttgcat
    95401 ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccgctta aggcaccaag
    95461 gtcggctacc gcatgaggtt tgccgctaat cggcttgctg tagcggatat gcgcatccgc
    95521 cagaataatc gttccgccga ggtggcgttc gcgcagcatc agccagataa gcccccaacc
    95581 ggtgagcgtt gccagcgaga ataaactccc ggcaaacagc gtgtgatgcg gattctgatt
    95641 gcctgtttcc ggcatggtgg tgataaattt ttgcccggta tattgctgaa tgcgcacgcc
    95701 cattttttca ctcagcggga tgtgttcata ccacgcctgt tgcagctgcg cgcaccagtc
    95761 gccgcgatgc aaaatatcat ccagagtggc aacgggttta atcatcaaaa aatggcgaat
    95821 cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca aaccccagct tggcgaaaaa
    95881 ctccaccgcg tcttcgcggg cgctacaggt cacgcgctta acaccttcct gacgcgccac
    95941 agattcgagg gtcatcgcca tcagcgttcc taaccctttg tcctgcacgt cgggatgaac
    96001 ggccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc ctaccgccac
    96061 cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc acgcgtcgcg
    96121 ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa actgatagta
    96181 acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga tactctctct
    96241 tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt cacggggcca
    96301 tcattgacca gcgatacctg catatccgca gcgaagcgtc ctgtttgcgt gttcatctct
    96361 tgctgacggc agcgttcgac gaaatagtca tataacgcct ctgcgcaatc cggtgatgcg
    96421 cctttggaga aacttgggcg catcccccgt tcggtatctg cggcaagggt aaactgggaa
    96481 accaccagta cactgccgcc cgcctgttgc acgttgagat tcatcttgcc ttcggcatcg
    96541 ctaaagatgc ggtagccgag cacacgctcg cacagacggt ttgctttctg ttcgtcgtca
    96601 tccttttcga cacccaataa caccaaaagt cccgcgccaa tttcacccgt cacttctccc
    96661 tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt tcgtcgtctt
    96721 cttcttgttc agcagcttgt tttaatttgc ggtattcccc gagagtgaca gtaatttccg
    96781 cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg gggatcaccg
    96841 ccagcacacc gtaaatgagc tgatatgatg ggaacatggt gatataaagc gcgaaacctt
    96901 tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc gacaatcgcg tcgcggttag
    96961 gtacacggat ggtaggaacg atgctgtaca gcagccagaa agagatccac gacaacagca
    97021 gcggaaaaat acgcaacaca ttatcgatga cagtattgag atcgctcgcc cagcgcagag
    97081 agagcagata ggagctgatc gccagactgg cccctgccaa cagcggcccc agcgttaaaa
    97141 tcatccagta cacggcaaac gagtaaattt ttggtcgcgc tcgtttactg cgccagatgg
    97201 tattcaacgc gctatcgatg gagtacatca acaataacgc cgtgacgatc agcccgcacg
    97261 ccccgacggc ggtcattttg ttggaattgg cgacaaactg ttcgatatat cgctggataa
    97321 catcgccagt agcaggcaga aagttggcaa aaataaagtg acgcaactga atgctgacgt
    97381 cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc ggcactaatg
    97441 agagcaacga cacataggca aggttacctg ccagggttgt catgttgtcc tcatcaatgc
    97501 gttgccagag tagttttagc caggcccata gtggacgggt acgatgcctg gctttgtcct
    97561 gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt tatctttcac
    97621 cagaatacta gtaatgccta gctgattagc tccttctata ttatcggcgt tatcgtcgaa
    97681 aaagaccgta tcgtctggtg aaaaaccttc tgcctgcaaa acatgctgat aaattcgtgc
    97741 ttcaggtttg cgcataccca agtcttgcga cagatagata tggtcagcag catcacggat
    97801 ttccgggtat tcctccggcc agaaggtagt atgcaggcgg ttagtattgg aaagcaccac
    97861 cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg ggcgcagcgc
    97921 aacaaatacc gcctgccaac cgtgggagaa ctgctcatag cttagcggca gggccatctc
    97981 atggcacagt gcctctgcga acgcttcgtc gctaatttcc ccacgctcat gctgatgaaa
    98041 cgcctcgccc atgtggaaac tcttcttaag cgtagccagt ggaacacgcg ttaaatcgct
    98101 ccaggccccc agcacacggt taaagtcgat atcgacaatc acattaccta aatcaaagat
    98161 atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg aaaggatggg
    98221 tttggctatg tggcaataag ggtaatcttg tgatgctgat tgcgaaaaag cagagtaatt
    98281 tcactggctt tttcgcaata gatgaatggt taagattcag gtgtaaattg ccaacgagtt
    98341 tcgatgacca gcggcttatc tggtgtcaca aattccggcg ttagatttat tgcgttaggt
    98401 gcctgagtca gcgggttaat acaggtggca tcgggctgtt tatcaaatac caccagactg
    98461 tttgcagaag aggtcatggt catcgcgagt ttaccaggcc acagtaactt aaccttcacc
    98521 ccgtcataaa aaccaaaaca gtcatcccag ggaccgactt gaggagagat acgatttcca
    98581 gttggcagtt catcactacc cgcttcttct tgccagtccg catcaaaaag cacctgcaac
    98641 gattctgtat tttgcggagt cagctttttc gcaaaccacg gatgccagcc tgcggaggcc
    98701 gggaatgtat cggcatatga atggacttcc agttgcaaaa ccagcgcatc gttctccagc
    98761 aaaaaagtct gaatcacttc cccttgccag ggccacggtg aagccagggg catacgtaaa
    98821 gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg agtaacaagc cataccatgc
    98881 aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt gcccaccagc attcagcgtc
    98941 gcattcccca gtcgccctgc ccacggcacc attgggaaac agccatactg aaaagctctg
    99001 cgctgcggct gccattggcg taatacttca gaaccaaatg cctttaacga ggttatcctg
    99061 gcaccatcat gcggataaat ctgcaggtga atattgcctt tttccagcgt cactaactct
    99121 cctacaggcc caggccatgg cgcgtcaaac gcctgttgta gcgccaggta atgctcgttt
    99181 tttcgttcag ttttcatgag attgttccct gatgtagcgg taagaagcgg tttcttgttc
    99241 atcggttggc tcaatcagcc agacgcaaac aaaggaaaga actgagacag caaataaaaa
    99301 cagagcgaca taccacggtt ttcctccccc tagcgccagc aaacttgagg cgataagggg
    99361 gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa gaaaggccgg tatatcgtac
    99421 tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg gcaaacatca tcgtcggacc
    99481 taagttgtag ccaaggatca tactgcacca gatgatgagc gtacttttgc tatccagtaa
    99541 taagaagaaa ggaaacgcga acaggacaca aaatcctgct cccgacaaat acagactttt
    99601 ttgacctata cgatctgata accatccaac caatggacaa ctgaaaatac cgatcgccga
    99661 ggcaatcaac atacccgtca ggggaatatc tcgcgacaat gctaattggc tggaaatata
    99721 gacaatacca aaggcgttaa taatattaga ggagacactt tccgccagcc tcgcacccag
    99781 tgccaataaa atatttcgtg gatgtttttt gaataattca atcaacggag gaacggactt
    99841 ttctttatta ttcgtcgttt tttgcttttg aaaatccagc gtttcttcgg tatgcaggcg
    99901 gataaacagc ccaacgatca acatcaccgc agaaagccaa aacggaatgc gccatcccca
    99961 ggagagaaac tgctccgaag taagaaaatg gttacagaga gcaaaaatac cggttgccag
   100021 cataataccc acagacgcag ccgtttgcgg gagtgaacct aaaaatccgc gtcgggattc
   100081 gggcgctgat tctatggtca tcaatgccgc tccgccgtac tcgccaccaa gaccaaagcc
   100141 ctgaatcaaa cgcagcacca ttaatacaag cggtgcccaa atgccaattt cctgatacgt
   100201 cggaataaaa ccaatcaaaa atgtggagca accgacaata gccagcgtcc agataagtgt
   100261 tatttttcgc ccgattttat cgccaaaatg accaaagacg atgccaccta aaggacgagt
   100321 cagaaaacca acaccaaagg tcgcaaagga agctataaga cctataaaag gatcgctgtc
   100381 tggataaaac agcggtgcaa ataccagtcc tgctgccgtg ccaaaaataa agaagtcata
   100441 ccattccatt aacgcgcctg cgaaactgcc cataacgaca cgtctcatgg ctttaccgga
   100501 aagcccttca cgcggtgcgg gtaacttttc agacataatt ttcctctcct ggtaggcgag
   100561 aaatctggga tacgaaccac taaacgtggg cagtttgaat taatgcgcat cggcttaaac
   100621 cgatgcgcat taaattattt cttcaattca gccagaacct ctgcgtcctc agcatcaaca
   100681 tttgcaccca gtggttccca attaatcaat acacaggtca ggaatcccag gaatggcacg
   100741 attgccaacc agtaaaacgc ttcagttttc aacgcacccc acagcatcgg ccagaaaatg
   100801 agtcctgcaa ttgcccctgt gcgcataata gcgcgggcaa atccgacccc agtagggcga
   100861 atagcgggag gataagagag tgtggcaatg gtcatgccca gcccaccagg acccgctgaa
   100921 tggaagaaaa taattgatcc caataaccct aaagacagcc agggattagt cgttgccagt
   100981 gcgcctaacg acagcaatgc taaggtcaca acagcaaatc catacatcga ctgccggcga
   101041 gtacccagtc gttggagaat aaacgagccg atcatcccgc ctgtcacccc acaaagggca
   101101 ttcacgacgg cagaaccggt taacgcgcca gtaagcccgc cacttattat tcccgccaac
   101161 gtaagaggaa gataaacacc tacggcgtta tattgccacg cctgcatggt tgccacgaca
   101221 cagccaagaa tggtgcgttt gcggtaacga tcattaaata agatgccata tccaccactg
   101281 tatttattct ctgctttttc cgagaatttc gcttctgttg tacccgatgc cgcaacgcga
   101341 gcctgaacac cataacgttt tcccagaatg tcgcacgctt cctggtaacg cccgacgcgt
   101401 gcggcccaca ttgcggattc accaataaag aagtaacgca aaatcatgaa gatgactgca
   101461 aaaatagctc cgatgaatat tccgtaacgc cacaattgtt cttcagcgat acccgacaga
   101521 agcagcggca aaagcaccaa ataaacaacg gttgtcgaaa cataccaggc catttgccag
   101581 aggttgaccg acgtgccggt tttcttcgac gttgaaccac gaagctctgc cacggcattg
   101641 gttgccaaag ggaagtcgat gcctaaacca aagcccatga tgacgcggca caccagcacc
   101701 caccagatac tgggagcaat agctaccgct gcagcaccga tggtgcacag ccccatgcca
   101761 ataataaatg cccgcttttg cccaaagcgg tttattactc ggttcgctaa taaaccgcca
   101821 attaacgcgg cgattaatac tgatgcattg acggttgcag caagacccgg cgaaatgccg
   101881 aattgagcgg caatgtattt attaccaaac cccaccatcg ccgcctggta ggcatcaatc
   101941 aatatgccgc ccaaagcaat aaaaataacg ccgatactgg tgttgattgc agagttctta
   102001 ttaacaatat caattacgtc ctgtggccga ctaattgaat aagttgcagg actggtgatt
   102061 tgactcatcg cagtgtcctt tttaattgtt gtagggtggc gctgacgtga cgcagcgcca
   102121 gacagagatt tagaaaatca ttccggcgca aattgcagca tgacctttat ggagtcattt
   102181 tgctgcgcgc gttcgaatgc cgccgctgca tcctcaatgg caaataaatc ggtaaccagt
   102241 tcactggtat caaaccgccc atccgccagc cattcgataa cagcatcgaa atcttcaggg
   102301 atgtacatgc cagagttgag caagtcacgc tcgaaacgtt gcatcatcgg taatggtatt
   102361 tcacgcggcc cacttggtac gcccatgcac acgacggtgc ctccggcata tacacgggta
   102421 catgcatcgt taagcgtggc ctgcgctgca acgacatcaa tcgcccccgt aaatcgcaca
   102481 tctgcggcca gctcacctgg tgcccaaact tctgctgcgc ccagttttaa cgccagcgcg
   102541 cgtttggcag cgtcaggttc aatcacggta atcttacctg cccccataat gcgcagtgcc
   102601 tgcacgatgg ataagcctat cgttccggca ccaatcacca gtacgctttc cagagaagct
   102661 ttcggcattc ggttaacgca gtgacgagca caagccgccg gttcggcaaa ggccaacact
   102721 tttagcggta aagaggctgg tgcgacatgg caattacgcg caggaacaat gtgttgtgag
   102781 cgagcaaagc ccggtgcgcg gaaaccagca acctgcggtg gttcacaaag attaaaacgt
   102841 cctgctttgc aggctcggca ttccatgcaa gccatgatgg gatcgaccac aacatgatcg
   102901 cccggctgta catttttaac gtcgctgccc acttccgtaa cgcgcgctgc aatttcatga
   102961 ccggggacga caggcggttt ggcaaacgga tgaccgcctt tcagaacatg cagatccgaa
   103021 ccgcatatcc cgtagaacac gggcgcaatc cgtacttcgt gcggtttaag ttgctggtgt
   103081 ggtacttcaa catattgagt ggtgactttc ccaatatctg aaaataaaac ctgggtaatt
   103141 ttatccattg ttattctcca gaatcgatat ccatagggga ttagcgttgc gcgatacctt
   103201 catcgacaat ttcagccagc cgttgcaagc gtggaacgga gacatcgcgc agcatggttt
   103261 gtggatcttg tgcaccaaca accgacgccc aaaccgctcg tccagccagg aagcccgacg
   103321 caccaccttt catggcaata cttactgcac ggccaaaaat atcggcatca acgccagaag
   103381 aaagaattac ccacggcatc ttcatctgat cgttaagttg ctgacaggca gcaagtaagg
   103441 ttttttcatc gcctttgccc cctaatggca tctcggcttt gtaaagatca gcctctgttc
   103501 caccaagctc tgcggcggcg gcaacaatgg cgctttcacg atcaaagtcc caaccgcgac
   103561 gaggtggacg caccacaggt tcaataatgc taaccagccc ggcactacgg cagcgacgaa
   103621 caaatttatc aaccattgcc agacgttcct ctgcgggttc atcttcacgc cagagtacaa
   103681 gtaatttcat tgccttagcc cccatttcac gggctttatg cgggtcgacg gacatatcta
   103741 tttcaacgct atcaaccggg atgccattac ctgggataaa aagatcggca gcgacaatca
   103801 ggccgcaaga attagccaca gcgccctgct cgactatttg ttcaagacaa aattgtttat
   103861 ccgccagaac ggcagaagca taaggagaaa ggattcgggt cgcggcaact ttaaaatcgg
   103921 tgagtacact gtcggcgatg ggttttggct gccctgctgc cgcaaacatc aggcgcatgg
   103981 cctcacgctg atcgacagct aacatggcga atccaccacc tgggcgggag atatctttca
   104041 gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt taatagataa aattaaagac
   104101 cagcctgctg gcgaacctga tttagcaggg cgctccagtc ctgccgccct ttgccgtctt
   104161 gacttgctgc ggcgtaataa ctttctgctg caatccccat attgagcggc gcccccgttt
   104221 cccgagcgac gtcaactgca atacgcagat ctttcaatgc caggtccacc atgaaggcgg
   104281 gagaaagatc gcctttcagc actttgccag gccaggtggt cgtaaaatgt cctttccctg
   104341 ctgccgtacc actcatcacc ttaattgcga catcaagatt taatcccagt gactcacata
   104401 acacagcggc ttcggaggac agcgcattaa gcgcaatgct catatagttg ttaattagct
   104461 tcacgcggat ccccattcct gggccaccag cctcgaccag ctcattaccc atgcacatga
   104521 gaatcgggcg tgcacgcgca atctgctcat cagtgccgcc cgctaaaatc agcaatgtgc
   104581 catcaatcgc attcactgac gttctgccca cgggggcgtc catcatattg atgccttttt
   104641 cttgtaattc ccgtattaat gcatcggtct gtaagggatg aatagtcgac atatcaatca
   104701 ctaacgcgtc agatgaaaca gtttcacaga cgcctttttc ccccaacagc acctgtcgaa
   104761 caatgtcccc attgggaagc atagtgatga taaattcagc gcttgctgcc gcttccgctg
   104821 gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc gacggcctgt gtattgacat
   104881 caaatacctg taatgaatgc cctttttgca atagattgtt agccattggg ctacccatct
   104941 gccccagccc gagaaatgca atggttgcca tgaatactct cctgtctgac attgacaaaa
   105001 taatgacaat tgaaaattcg ttttcgtctg tttttgagca tatttgtaat ttatgttcaa
   105061 aaacacgaca cgcatcacta atttgatcat ttgtgtcatt taaattgacc aaagacgaca
   105121 aaattaaaca ggacaagaca aatatgacca gaattgcatg cgtgggtatc acagttcagg
   105181 acaggattta ttctttgccc actctgccgg agggcggcgg gaaatatcag gcaaatcatt
   105241 atcttgagat tggtggcggc ccggcagcaa cagccgctgt tgcaatcgca aagcttggcg
   105301 tggaggttga tttcatcggt cgcgtcggcg atgacagttg tggaaatacc ttacttgcag
   105361 aacttgaggg ttggggcgtc aataccgcat tttgccgccg atatcctaac gcccgttcgt
   105421 cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat tatcgttaat tatccaagtc
   105481 ctgatctcgg tactgatgcc gaatggttag aagcgatcga tttctctcgt tatgatctga
   105541 ttctggccga tgttcgctgg catagcggta ctgaaaaagc attctcgcta gcgcgccttg
   105601 cgggtgtgac aacgctttta gacgccgata tgacaccgca ggatatttct ccacttgtcg
   105661 cgcttgccga tcacgccgtg ttctcaacac ctgggctgaa acgcatgact ggcctgcagt
   105721 cacctgaaga aggtttgttc caggcaacga cacaaacagc aggtaaagtg tatgttacgc
   105781 tgggttctga aggatcgttg tggattgaag atggtcattt gtgtcaacag gaggcatttt
   105841 ctgtcaatgt ggttgatacc actggagcag gcgatgtttt tcacggcgca ctcgcggtag
   105901 cgttggcaga aaaaatgcca acaaaagagg ctattcgttt tgccagtgcg gttgccgcga
   105961 tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa tcgtgagcaa accgaatcat
   106021 ttttgtcact ttatgcgtaa aatggtaaac atctaccgag gaggggcaag ggagtattca
   106081 tgagtatcat cgaagtgaca ggtaatccac gccatgacca actcgttcat ctaatcgcag
   106141 aacgcggtta tatgaatatt gaagaactgg cacaactgct ggatgtttca acgcaaaccg
   106201 tgcgccggga tatccgaaaa ctcagtgagc agggcttgat cacccgtcat catggagggg
   106261 ccgggcgcgt ctccagcgtc atgaataccg cttttgagca acgggaactt tcgctcaccg
   106321 ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct tcccgaacgc tgtaccgtct
   106381 ttatcaccat cggtacaacc gtagaagccg ttgccagggc attactcaac cggcgtgatt
   106441 tacgcattat cactaatagc ctgcgtgtgg cacagattct ttataagaat caggatattg
   106501 aagtgatggt gccgggaggc actttacgcg ctcataacgg tgggattatc ggcccaggag
   106561 ccgtggattt tattgaaggc ttccgcgcag attatttaat caccagtata ggtgccattg
   106621 aacatgacgg caccctactg gaatttgatg tcaatgaagc gttagtcgcg agaacgatga
   106681 ttaaacatgc gcggaataca ttgttagtgg ccgatcatac gaagtttacc gcgtctgctg
   106741 ccgtttcaat tggcaatgca cggaatgtca gggctttttt tactgatgcc ccgcctccca
   106801 attctttctg ccagttgtta agtgaagaga atgttgaact ggtggttgcc gagcaagaag
   106861 tatcctgaaa ttgaaacttc ggttgatacg gttttcgttg gccggattaa gcacatccgg
   106921 ccactaatta agcttcagcc gccagcatct ctttcttacg ttggctaata cgcttaacca
   106981 gcattacgga aaccaccgca ccacagaatg cgaagcaggc cataatgcca aacacgattt
   107041 tgtagccaat aatccccgga tttaaatcca gaatgtagcc atacagactg aagcagaaca
   107101 tcgccggggc gtaaccaata aagctaccca acgccatcgc cgcgcctgtt ttattttcag
   107161 caatttttgc ttcgccgata ggtgcaaaaa atacggctcg ctgtgtgaag actatagcgc
   107221 caaagcccag cgtacatgcc atccctaaat agaccggcat actttcgtgc ggcagcataa
   107281 tcaacagtac gagcgcagcg gtacttatga taaaggtgta gcatagatat ttactcggcg
   107341 atttcaggat cttatctgaa atcatgccac caatcggtcc tccaatcatt ttcagacagt
   107401 attggttaat gatgccgtaa gcccccacca gagcaacggg caatagatag atgtttttca
   107461 ggaatggaat gaagaatgtt aggccacagt aaaccgcgta aacgaagaag acgttgaaag
   107521 cgataagcca gatggtttta tctctcagca ccgaggtcat actggtgttt tgcgatgctc
   107581 cgtcttcttt tttcacctca acggacggtg cctcttcttt gtcattcagg acaaagaaaa
   107641 tgataatgcc tacggcaatc acaataaggg aatagaacca gatgcctgct ttaaaaccca
   107701 ataagccact gccaaaccag gtaaatactg ccaacgcaga aaatgccacc acggtatcga
   107761 caatgccacg ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg ctattgccca
   107821 atcgacttac cgacttgagc aagaccggcc agttcatcat gtcgcaagta acgccaaata
   107881 gcgcccagac aaagaggatg ccccaatacc ccggcattgt cgtcaaataa acacccagta
   107941 atcctgtcgc aatgagtgag aatgacatgg tgtatctgcg cggtagttta tcggcaaaat
   108001 aaatagacag aaaaaagccc actgtggtga caaatgagtt taccgacata gcattaccaa
   108061 tttgaccatt ggtcaaatgg aaatattcct gcatcgggat ataaaacgca tctttcagag
   108121 acggtaattt ataaattgta ccgccacaca atgttaatag actaaataac gcccatttct
   108181 ttttcgtgag cataacatcc cctaagcgat ttttaaaatc actgttttaa aggcaataag
   108241 ataccgacca ttcatatgga atggttttat taacagcgta tgagttaaaa cttattagtt
   108301 gttcagaaga tggtttgcct cttgtttgat ttgttgtagt acattacgca catgatttat
   108361 ctgattaatt gcatcttctt tttctttacg taaccgcgca tccagtaaat gattttctgg
   108421 caatggtgtt tcactcatct ggcgataagg gatccacgga ttatcatctt cgtcttcaaa
   108481 gcggaacgcc ggcgcatagt aatcaacctc ttcttccagg ccatatgccg tcacctgcgg
   108541 ctcatcatca cccaggcaga taaggtgcgt taataacgct ttgaacggca tatcaccctg
   108601 acctgaaata caggctttat gacccaggcc accctcttct ttaacgatca aggcatcttt
   108661 gatatggacc tgggtaatat gcggtgccat cgtttttaaa gcgtcgatgg gatgctcatt
   108721 tgcattgatc atgttcgcaa aatcaaacag taaggaaaga gattccatct cactttcttt
   108781 gaccagcgac accagctcat ggctttttaa atcttcatgc tgttcgatcg taaaagtcag
   108841 gccgctgtcc tgatacgttt cccgtacata ggcaatgtcg ttagcgatga tcgataatac
   108901 gtcgcgcaaa ttaccttcat aacgtggata aaaacgtacg gacgatgccc cagttttcaa
   108961 cgcaatggcg acggcttcgt caatagatgc cttatctgaa gcgctggttt caatatgaat
   109021 atcaaggttc agacggcggg ctttatcccc aaaggcagag agttctttat cgtccatatt
   109081 accaagagaa aaacgctcgc catccagaac atggattttg acgccacgta gattattttc
   109141 acttgcgata tcaagtaaat cagcaggcaa cactctttcc agccgcatat ttaaatgaaa
   109201 ggcataagca tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct ttcttgcgtt
   109261 atttattgtc accataaaac acctacagat taatgatgag gtctacggcc tcaaatcgcc
   109321 aatattcaat atctatctgg acaatattgc cttgctggtc ggcgcgatgc ttttcaatta
   109381 agatcgctgg catacccgca gaaccgccaa gatacttgct gatatcgccc ggcattctga
   109441 cgggtttgaa aatcaatttt tttactaccg tctctttttg gtagcacttt tcccagactg
   109501 cagaaaatga gtggttttca agttgttcaa taaaacccgg ggcaacttca ggattaatat
   109561 atgtttcgtg atagaaaact ttatgtcctt ccagcgctcc ccagccggtg atgcgataga
   109621 gttcagttga tggtgtcact gtaatcagtg gcacgagcgt ctcgggaata tctctggtac
   109681 ggcttttctc ggtaaacccc caggaaggct cccgcccttg ctcaattgca gcccgctgaa
   109741 agctcgccga cagctctgga ctgtaattaa atcgcggctg ggtcacgaac caccctttac
   109801 gatccttacg gaagattttg gattctgcct cgaggttcaa caacgcctgg cgcagcgtca
   109861 tacgtttaat gcccagcagt tctcccagct ctctttctga agggagtttt ccacccggcg
   109921 tggtgatgcc atctttcaac caccgatcca gtttctcttt tgcattttct acggtggatt
   109981 gattctccgc cattgtttaa tccttttggt ttaaaccaga tgaggcatct tacgcttacg
   110041 ccaaaaaaga aagcaattaa cggcgtatga ctttgcagat aattgtgaca aagctctcta
   110101 ttgacgtaat cacagactta taagggagat actcgcattg gtgtgcgctc acgacaggta
   110161 aaaaaaaacc tgccagcgat ggcaggtttt ttatgactga aaagcgaaat taatcgtctt
   110221 tcggtgagcg gttggcgcga cgacgatcgt tttccgtcag gtgacgttta cgaatacgga
   110281 tagaggtcgg agtcacttct accagttcgt catcatcgat gaactccaga gcttgttcca
   110341 gagtcatgcg gataggcgga accagaacaa cggcttcgtc agtaccggaa gcacgcatgt
   110401 tggtcagttt cttaccggtc aggcagttta cagtcaggtc gttagagcgg ctatgaatac
   110461 cgataatctg accttcgtaa acttctgcac cgtgaccgag gaacagctta ccgcgatcct
   110521 gcagaccgaa cagcgcgaac gcgaccgctt taccctgacc gttagagatc agtacaccgt
   110581 tctgacgctg acccacttcg cccggacgta cgtcgtcgta gtggctgaag gtggagtaca
   110641 gcagaccagt accggaagtc atggtcatga actcagaacg gaagccaatc agaccacggc
   110701 ttgggatcac gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg tttttcaggt
   110761 cgcctttacg ttcgcccagc gcctgcatca cagaaccctg atgctgttct tcaacgtcca
   110821 gcgtcacgtt ttcatacggc tcttgtttac gaccgtcgat ttcacggaag attactttcg
   110881 gacgggatac cgccagttcg aaaccttcac gacgcatgtt ttcgataaga acagacaggt
   110941 gcagttcgcc acgaccagaa acgcggaacg catcggcgtc ttcggtttct tctacgcgca
   111001 gcgcaacgtt gtgtaccagt tctttgttca gacgatccag gatctgacga gacgttacga
   111061 acttaccttc tttaccgcag aacggcgagg tgttaacgca gaagaacata gaaacggtcg
   111121 gctcatcaac ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa acggtgtcag
   111181 aaatgttcag ttcgcccagg cccgtgatcg ccacgatatc gccagcttcc gccagatcgg
   111241 tttcgatacg ttccagaccg aggtggccca gcactttacc gactttcgcg ttgcgggttt
   111301 tgccttcgct atcgatgata gtaacctgct ggttcggctt cactttaccg cgcttgatgc
   111361 ggccaatgcc gataacgcca acatagctgt tgtaatcgag ctgagaaatc tgcatctgga
   111421 acggaccgtc aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc tgatacagcg
   111481 gggtcatgtc ttccgccata tcttcgtggt ccagacccgc gataccgttc agcgcagaag
   111541 cgtaaacgat cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg aacagatcga
   111601 atacctgatc cacaacccaa tcaggacgcg cgccagggcg gtcaactttg ttgataacaa
   111661 caatcggctt caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt tgcggcatcg
   111721 ggccgtcaaa tgcgtcaacc accagcagca ctgagtctac catggacatt acacgttcaa
   111781 cttcaccacc gaagtcggcg tgccccgggg tatcaacgat gttgatacgg taatcattcc
   111841 atttgatagc ggtgtttttc gcgaggatgg taatcccacg ctctttctcc aaatcgttgg
   111901 agtccatcac gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat tgttggagca
   111961 gcttgtctac cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg atattacgca
   112021 atttttcgat cacaactttg cctcaggcat tagaaatagc gcgttattgt acacggatta
   112081 atcgcactac aaaacaggat cacaaacatc ctccgcaaac aagtattgca gagtcccttt
   112141 gtgatcgctt tcacggagca taaaaagggt tatccaaagg tcattgcacc aatatggtgc
   112201 ttaatgtttc cattgaagca ctatattggt gcaacattca catcatggtg cagccctttt
   112261 gcacgatgtt aagcatgata acgcctttta ggggcaattt aaaagttggc acagttttcg
   112321 ctttatcttt ttacggcgac acggccaaaa taattgcaga tttcgttacc acgacgacca
   112381 tgaccaatcc aggagagtta aagtatgtcc gctgaacacg tactgacgat gctgaacgag
   112441 cacgaagtga agtttgttga tttgcgcttc accgatacca aaggtaaaga acagcacgtc
   112501 actatccctg ctcatcaggt gaatgctgaa ttcttcgaag aaggcaaaat gtttgacggc
   112561 tcctcgattg gcggctggaa aggcattaac gaatccgaca tggtgctgat gccagacgca
   112621 tccaccgcag tgattgaccc gttcttcgcc gattctaccc tgattatccg ttgcgacatc
   112681 cttgaacctg gcaccctgca aggctatgac cgtgacccgc gctctattgc gaagcgcgcc
   112741 gaagattacc tgcgttccac tggcattgcc gacaccgtac tgttcgggcc agaacctgaa
   112801 ttcttcctgt tcgatgacat ccgtttcgga tcatctatct ccggttctca cgttgctatc
   112861 gacgatatcg aaggcgcatg gaactcctcc acccaatacg aaggtggtaa caaaggtcac
   112921 cgtccggcag tgaaaggcgg ttacttcccg gttccgccgg tagactctgc tcaggatatt
   112981 cgttctgaaa tgtgtctggt gatggaacag atgggtctgg tggttgaagc ccatcaccac
   113041 gaagtagcga ctgctggtca gaacgaagtg gctacccgct tcaataccat gaccaaaaaa
   113101 gctgacgaaa ttcagatcta caaatatgtt gtgcacaacg tagcgcaccg cttcggtaaa
   113161 accgcgacct ttatgccaaa accgatgttc ggtgataacg gctccggtat gcactgccat
   113221 atgtcgctgg ctaaaaatgg cgttaacctg ttcgcgggcg acaaatatgc aggtctgtct
   113281 gaacaggcgc tgtactacat tggcggcgtc atcaaacacg cgaaagcgat taacgccctg
   113341 gcaaacccga ccaccaactc ttacaagcgt ctggtcccgg gctacgaagc accggtaatg
   113401 ctggcttact ctgcgcgtaa ccgttctgcg tctatccgta ttccagtggt ttcttctccg
   113461 aaagcacgtc gtatcgaagt acgtttcccg gacccggcgg ctaacccgta cctgtgcttt
   113521 gctgccctgc tgatggccgg tcttgacggt atcaagaaca agatccatcc gggcgaagcg
   113581 atggacaaaa acctgtatga cctgccgccg gaagaagcga aagagatccc acaggttgct
   113641 ggttctctgg aagaagcact gaacgaactg gatctggacc gtgagttcct gaaagccggt
   113701 ggcgtgttca ctgatgaagc gatcgatgcg tacatcgctc tgcgtcgcga agaagatgac
   113761 cgcgtgcgta tgactccgca cccggtagag tttgagctgt actacagcgt ctaatagttg
   113821 aagttgtact acccggcgca acaacgccgg gatttagttg ccgtggaaac tttcagccca
   113881 tctctgcatg ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa
   113941 aagctataat gcactaaaat ggtgcaacct gttcaggaga ctgctttatg gcaacaggca
   114001 cgcagcccga tgctgggcag atcctcaact cgctgattaa cagtattttg ttaatcgatg
   114061 acaacctggc gatccattac gccaaccccg ccgcgcaaca actgctcgcc caaagctccc
   114121 gcaaattgtt tggtacaccg ttaccggaac tgttgagcta cttctcatta aatatcgagc
   114181 tgatgcaaga aagtctggag gcggggcaag gttttactga taacgaagtg acgctggtca
   114241 tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg tatgccggac ggcatgatcc
   114301 tgctggagat ggcaccgatg gataaccagc gccgcttaag tcaggaacag ctacagcacg
   114361 cccagcaggt tgctgcccgt gatttagtgc gcggcctggc acatgagatt aaaaatccgc
   114421 ttggcggttt acgtggcgcg gcgcagctgc tcagcaaagc attacctgac ccgtcactac
   114481 tcgaatatac caaagtgatt atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc
   114541 tgttgggacc gcaactgccc ggtacgcgcg ttaccgaaag tattcataaa gtagctgaac
   114601 gcgtggtgac gctggtgtcg atggaactgc cggacaacgt gcggttgatt cgggattacg
   114661 accccagcct gccggaactg gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata
   114721 ttgtgcgcaa tgcgctacag gcgctggggc cggagggtgg tgaaatcatt ctgcgtaccc
   114781 gcacagcgtt tcaactgacc ttacacggcg agcgctatcg gctggcggcg cgaattgatg
   114841 tggaagataa cgggccaggt attccgcctc atttgcagga tacgctgttt tacccgatgg
   114901 tcagcggccg cgaaggtggc accgggcttg gcttatccat cgcccgtaat ttgattgatc
   114961 agcattcagg caaaattgaa tttaccagtt ggccaggtca taccgagttc tcggtttacc
   115021 tgcctatcag gaaataaagg tgacgtttat gcaacgaggg atagtctggg tagtcgatga
   115081 cgatagttcc atccgttggg tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac
   115141 aacatttgag aacggcgcgg aggtactgga ggcgctggcg agcaaaacgc cggatgtgct
   115201 gctttcagat atccgtatgc cgggaatgga cgggctggcg ctgctcaagc agattaaaca
   115261 gcgccatccg atgcttccgg tcatcattat gaccgcacat tccgatctgg atgctgccgt
   115321 cagcgcctat caacaagggg catttgatta tctgcccaaa ccgtttgata tcgacgaagc
   115381 cgtggcgctg gttgagcgcg ccatcagtca ttaccaggaa cagcagcagc cgcgtaatgt
   115441 tcagcttaac ggcccaacga ccgatatcat cggtgaagcg ccagccatgc aggacgtgtt
   115501 ccggattatc ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg gcgagtccgg
   115561 caccggtaaa gaactggtcg ctcatgccct gcatcgccac agtccgcgag ccaaagcgcc
   115621 gtttatcgcg ctcaatatgg cggctatccc gaaggatttg atcgaatccg aactgtttgg
   115681 tcacgagaaa ggcgcattta ccggcgcgaa taccattcgt caggggcgtt ttgaacaggc
   115741 tgatggtggc acattattcc tcgatgaaat tggcgatatg ccgctggatg tgcagacgcg
   115801 tttgctgcgc gtactggcag atggtcagtt ttatcgcgtt ggcggctatg cgccggtgaa
   115861 agtagatgtg cggattatcg ctgccactca ccagaatctt gaacagcggg tgcaggaagg
   115921 taagttccgt gaggatctgt tccaccgcct gaacgtcatc cgcgttcatc tgccgccact
   115981 gcgtgagcgt cgggaagata ttccccgtct ggcacgccat tttttacagg ttgccgcgcg
   116041 cgaactgggc gtagaagcga agttgctgca tccggaaacc gaagccgcgc tgacgcgcct
   116101 ggcgtggccg gggaacgtgc gccagctgga aaacacctgt cgctggctaa cggtgatggc
   116161 cgccgggcag gaagtgttga ttcaggattt gcccggtgaa ctgtttgaat caacggttgc
   116221 ggagagtact tctcaaatgc aaccggacag ttgggcgaca cttttagcgc agtgggcaga
   116281 cagagcgctg cgttccggtc atcaaaatct gctttccgaa gcgcagcctg agctggagcg
   116341 gacgttactg acgaccgcgt tgcgacatac gcaagggcat aaacaggaag cggcgcggct
   116401 actcggctgg gggcgtaaca ctctgacgcg gaagttaaaa gagctgggga tggagtgatt
   116461 cacggcttgt gtgtaaagat tgattattga gcgcaaattg ctggtgtttt gcgctttact
   116521 gttccgataa gttcagtatg atcttgcccg gaaaacgggg agagtcatta tgctggaatc
   116581 aataattaat ctggtatcga gtggcgcagt tgacagccac acaccgcaaa ctgctgttgc
   116641 tgccgtgctg tgtgccgcga tgattgggct gtttagttga gtttcagatt gctgatgaag
   116701 tgctctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa aagcaatatt
   116761 ttcaacagat tttgcaggcc tgataagcgt agcgcatcag gcaatcttgt tctcagcaca
   116821 ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc attcacaatt taaatcaccc
   116881 gagagaactg ctgcatccgc gctttctggc gcagataggt atcaaagcac atgcaaatgt
   116941 tgcggatcag caagcgacct ttcgccgtca cctgtattcc cttctcatcc acatccacca
   117001 gcccatcttt tgctaacggg gcgagcagct tgagatcttc ggcaaagtaa tcagcgaaga
   117061 gcaaatccca ctgttgctca atgggagcgt aatccagacg gaagttgcag atgagcgact
   117121 taatcacatc gcggcgaata cagtcatcac gcgttagcgc aataccacgc cacagcgcat
   117181 tgccttgttc atccacttgc tgatagtact gcttcaactc tttctggttc tgcgcgtagc
   117241 agtcgccaat catgctgatg gcggaaacgc ccatccccag cagatcggta tcgccctgag
   117301 tggtgtagcc ctggaagtta cgatgcagca cgccttcacg ctgggccacc gccagctcgt
   117361 catccggacg ggcaaagtga tccataccga taaactgata gcccgattgt gtcaggaagg
   117421 cgatggtttc ctgcaggata tcgagttttt gctgcggact cggcaggtca gcatctttga
   117481 ttttgcgctg agcagcaaaa atggtcggca gatgcgcgta gttaaagaca ctcagacgat
   117541 cggggttcag ctccgccaca cgtttcaggg taaaggcgaa actctccggc gtctgtttcg
   117601 gcaggccgta aatcaggtcg atgttggtgg aggtaaagcc aatctcacgc gcatggttaa
   117661 gcagtgcaaa gatgaactct tcatcctgct cgcggttaac cagacgttgc acttctttgt
   117721 tgaagtcctg cacgcccatg ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta
   117781 catccagttc gatttcccgc ggatcgactt cgatcgagat ctccgcatcg gcattgaact
   117841 ggaagttttc gcgcagcagc ttcatcagac ggctgatttg cgctttattc agatacgtcg
   117901 gcgttccgcc gccccagtgc aattggctga cgtgacgccc ggcgaacagc ggtgcacgat
   117961 ggacgatttc ttgctccagc gcgtccagat actgatcggc cttgtgctgc tggcgagtaa
   118021 ctatcttatt gcaaccgcag aagtaacaaa gcttatggca gaacggaata tgtacgtaga
   118081 gagataatgg acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt
   118141 cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa tagttatatt
   118201 tctggatcag ggccagatcc cagtcgattt gctgtacaga catgctcact ccttccgatg
   118261 gcgtctctgg cggctacggc gtaccggcgg atgcccgttt cgagtcatca gccggttgcg
   118321 cagccacttt tgtcgccgcg acaaccgctg tagtttaacg aataaccaca ccagataaca
   118381 tataaccgga agggttatca gcagaacggg aaggcccacg gcggcaatcc gttagttgcc
   118441 ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc
   118501 ataagagagg ccgagtttct gcatcaactc atcaatacga tccagtttgg catccaccca
   118561 ggattgctct tcggcgctca gggtttcgcc tgcttccaga cgttccagca gcgcatccag
   118621 acgctcatcc gtttccagta actccaactc cgcctgcggt gaaagcatag gtttctcact
   118681 cttcggtttg tgctgtttgg tgactttttc agtcacgccc aatggaatag gggttttact
   118741 gccaatacgt ggatctttcg gtgcgttctg gcctttgctg cctgacgtgg tgttaccgcc
   118801 cgctgcgcgg ctgcccggcg catgaccacg acgttttttc tgacgcttac ggtcacgagc
   118861 ttcctgatcc agctcctcgc gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga
   118921 gtttgaagat gatggtttca taatggttcg tcttttagcc gttttattca gtatagattt
   118981 gcggcggaat ctaacagaaa gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt
   119041 ttttcctgac tcataaccct taacgggtct tacaatcctt gcaggccgcc aacacaccct
   119101 gtttttccat tagccggaac aatccgtgtt gattccattt ccgtttaatt acgtctccgg
   119161 acgtttatct tcctgacaat cctctgtctt cgcctcctgg cgctcctgac cctcatcctg
   119221 agtcattgtt cctgttagcg tcctcgctct atgggtgcta ctttacgcct ttgctttaaa
   119281 taaacaatca accgccatca gtatttgcac attttcagag ttttatataa aagcaattca
   119341 ctgatttata tagcttattt gacatacgtc gcagaatttt ctctgctatt tcgcataaag
   119401 actatcggca atgtctcaca aagcatatgg cttttactta caacgctcac gccgaaaaat
   119461 ccgcctttta ttatggttca ggtataatcg ccgcaaagct ttgactgatg gagacgaccg
   119521 ctttgactaa tttgaattat caacagacgc attttgtgat gagtgcgcct gatattcgcc
   119581 acctaccttc cgataccgga attgaagtgg cttttgcagg ccgttccaac gcaggtaaat
   119641 ccagcgcgct gaacacgctg actaaccaga aaagcctggc tcgtacctca aaaaccccgg
   119701 ggcgcaccca gcttatcaac ctgtttgaag tggctgacgg caagcgtctg gttgacttgc
   119761 ctgggtacgg ttatgcggaa gtcccggaag agatgaagcg caaatggcag cgcgcgctcg
   119821 gcgagtacct cgaaaaacgt cagagcctgc aaggtctggt ggtgctaatg gatattcgcc
   119881 atccgctgaa agatttggat cagcagatga ttgagtgggc ggtagacagc aatatcgccg
   119941 tgctggtact gctgaccaaa gcggacaaac tggcaagcgg cgcacgtaaa gcgcaattga
   120001 atatggtgcg tgaagcggtg ctggcgttta acggtgatgt gcaggttgaa acgttttctt
   120061 cgctgaagaa acaaggcgtg gacaagctgc ggcagaaact ggatacctgg tttagcgaga
   120121 tgcagcctgt agaagaaacg caggacggcg aataattttc ttgccttaat gcttgtgccg
   120181 gatgtggcgt atcaggcatt acggatcttt tctttcgccc aataaaaaac gccccagtca
   120241 ttactgactg gggcggctaa aatattcagc caaatccgat tacgtgaagt aaaaggtctg
   120301 aaagatagaa catcttacct ctgtacccta cgcgactaac tttactcttt tttgttcagt
   120361 tcagaaagca ttttttgtaa ttttttttca gttgttgcat aggaatttct aatagccatc
   120421 acaaaacgtg acagcttatg ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt
   120481 agtgcgcctg atcccagttt tcgccactcc ccacttccac cagcagcggc acatccagac
   120541 gggtacagtt ttccatcagt tgatgaatct gcttcgcgac ggcatcgaca tcatctttat
   120601 gaacttcaaa taccagttca tcgtgtacct gcatgatcat acgcacacgc ggttgctcag
   120661 cctgtagcca cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg gcggttccct
   120721 gcattggcgc gttaatggct gcacgttcag ccgctgcacg acgagcccca ttgctggatt
   120781 tgatatccgg cagatacaga cggcgtccgt ccagcgtttc aacgtagccc tgctctttcg
   120841 cctgagcacg ggtgcgttcc atatactcca gcacgccagg gtagcgttcg aagtaaaggt
   120901 ccatgtactt ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac
   120961 tcatgccata aatcagacca aagttgatcg ctttcgcgct acggcgttgc tcgctggtta
   121021 cggtttccag tggcaaacca aacacttctg ccgccgtagc ccggtggata tcttttcctt
   121081 ccgcgaatgc ggtcagcaag cctttgtcac gcgagagatg cgccataatg cgcagttcaa
   121141 tctgcgagta gtccgccgag acaatcacat aatcctctgg cgcaataaac gcctggcgga
   121201 tacgacgacc ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg gttgacgata
   121261 aacgtcccgt tgcagttact gcctggtgat aagaggtatg cacacgcccg gttttcgggt
   121321 tgatcatcag cggcagcttg tcggtgtaag tcgatttcag cttcgccaga ccacgatact
   121381 ccagaatcac ttttggcaac ggatagtcca gcgccagttc ttccagtacc tcttccgacg
   121441 ttgacggcgc gccacccggc gttttcttca gcggtttaat tccctgtttt tcaaacagaa
   121501 tggtttgcaa ctgcttggtg gaggaaaggt taaattcttc acctgcaatt tcatgcgctt
   121561 tcttttccag ctcagccaga cgaagagtga gctcttcaga atgattgtgc agcactttcg
   121621 gatcgatctt cacaccgtta cgttcaatgc gtgaaagcac cggcactagc ggcatttcga
   121681 tattctcgaa gacgttcaac ggccctttgt gtttttgcag atccggccac attttcagat
   121741 gcaactgcaa ggtgacatct gcatcttcgg cggcgtaacg tccggcctct tcgagggcaa
   121801 tctggttaaa ggtcagttga tttttgcctt tacccgcaat ctcttcaaaa gtgatggttt
   121861 tgtgcttcaa ccaacgttcc gcgaggctgt ccatatcgtg acgcccggca acgctattga
   121921 gaatgtagga ctccagcatg gtatcaaacg caatcccacg cagctcaatg ccgtaattcg
   121981 ccagaatacc gcgatcgtat ttcaggtttt gcccaacctt cagcgccttt tcatcttcca
   122041 gcagcggttt tagcaactcg agtgcacgct cgcgagagat ttgatcgggc gcatcaagat
   122101 aatcatgagc aaccggaata tatgccgcta cgcctggctc gatagcaaaa gaaagcccga
   122161 ccaggttagc agagatgtta tcaaggctgt cggtttcggt atcaaatgca aataccggcg
   122221 ctttttccag cttcgcaatc cacgctttca gtgtttcttc atcaaggatg gtgacgtagt
   122281 tgtcataaga aatcaccgtt gccgtcactt ctggcgcttc gtctacaaca ctggtttcct
   122341 gcggcttcgc ggctggtttt gcccctttgg cctgtaacca tttgcccgct tcgacatcag
   122401 cagtccagcg tttgaactca tactttttga acagccctaa caactcttct gccaccggtg
   122461 gctgcacttc cagttgttca caggtcagct ccagttcaac gtcggtttta atcgtcgcca
   122521 gctggtaaga gagataagcc acttctttgt tttgctcgag cttcgccgcc attgttttcg
   122581 cgccacggaa gctcaaccca gcaatttttt ctggctcggc atacagcgta tccagtccgc
   122641 caagaccttg tagcaatgct tgcgcggttt tttcaccgac gcccggtacg ccagggatgt
   122701 tatcagagga gtcacccatc agcgccagga agtcgatgat cagttctggc ggcacgccgt
   122761 acttattcac cacctcttcc gggccgagga tggtattcgt catggtattg ataagcgtaa
   122821 tatttggcgt caccagctgc gccatatctt tatcgcctgt gcttatcagc accggacgcc
   122881 cggctttttc ggcttcccgc gccagagtac cgataacgtc gtccgcttct acgccagaaa
   122941 ccgccagcag cggcagtccc atcgctttaa ccatcgcgtg caagggttcg atttgtgcac
   123001 gcagatcgtc cggcattggc gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa
   123061 aggtttttcc cttggcgtca aagaccaccg ctgcatgcgt cggtttatat tgcatgatta
   123121 gactgcgcag catgttgagg acaccataca tcgcaccggt cggctcgcct gcgctgttag
   123181 tcagcggggg aaacgcgtga tatgcgcgat aaagataaga tgaaccatct acaaggataa
   123241 gtggattttg ggggatctga accataatgt ccgtgcctgt ttatcagatt atgggtaaag
   123301 gatgccacag acaggatgaa aacatgagtt tttcaccgct tttcgtgaaa atcttttgta
   123361 gatcttctgg atcgctcgca aatcgtcatg tggataactt tgtgcataaa ttatttacat
   123421 cgcttttaat ttattatcat gctttttaca gcatgat
//
LOCUS       DAZGTJ010000003       113313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000003 DAZGTJ010000000
VERSION     DAZGTJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 113313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.003"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(73..1209)
                     /gene="hemW"
                     /locus_tag="ACHK8E_000254"
     CDS             complement(73..1209)
                     /gene="hemW"
                     /locus_tag="ACHK8E_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP0342580.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(1202..1795)
                     /gene="rdgB"
                     /locus_tag="ACHK8E_000255"
     CDS             complement(1202..1795)
                     /gene="rdgB"
                     /locus_tag="ACHK8E_000255"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP0342581.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(1803..2093)
                     /gene="yggU"
                     /locus_tag="ACHK8E_000256"
     CDS             complement(1803..2093)
                     /gene="yggU"
                     /locus_tag="ACHK8E_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP0342582.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(2090..2656)
                     /gene="yggT"
                     /locus_tag="ACHK8E_000257"
     CDS             complement(2090..2656)
                     /gene="yggT"
                     /locus_tag="ACHK8E_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP0342583.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(2674..3378)
                     /gene="yggS"
                     /locus_tag="ACHK8E_000258"
     CDS             complement(2674..3378)
                     /gene="yggS"
                     /locus_tag="ACHK8E_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP0342584.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            3396..4376
                     /gene="yggR"
                     /locus_tag="ACHK8E_000259"
     CDS             3396..4376
                     /gene="yggR"
                     /locus_tag="ACHK8E_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP0342585.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            complement(4550..4966)
                     /gene="ruvX"
                     /locus_tag="ACHK8E_000260"
     CDS             complement(4550..4966)
                     /gene="ruvX"
                     /locus_tag="ACHK8E_000260"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP0342586.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(4966..5529)
                     /gene="yqgE"
                     /locus_tag="ACHK8E_000261"
     CDS             complement(4966..5529)
                     /gene="yqgE"
                     /locus_tag="ACHK8E_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP0342587.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(5638..6588)
                     /gene="gshB"
                     /locus_tag="ACHK8E_000262"
     CDS             complement(5638..6588)
                     /gene="gshB"
                     /locus_tag="ACHK8E_000262"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP0342588.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(6601..7332)
                     /gene="rsmE"
                     /locus_tag="ACHK8E_000263"
     CDS             complement(6601..7332)
                     /gene="rsmE"
                     /locus_tag="ACHK8E_000263"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP0342589.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(7412..8119)
                     /gene="endA"
                     /locus_tag="ACHK8E_000264"
     CDS             complement(7412..8119)
                     /gene="endA"
                     /locus_tag="ACHK8E_000264"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP0342590.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(8214..8711)
                     /gene="yggI"
                     /locus_tag="ACHK8E_000265"
     CDS             complement(8214..8711)
                     /gene="yggI"
                     /locus_tag="ACHK8E_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP0342591.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(8788..10182)
                     /gene="galP"
                     /locus_tag="ACHK8E_000266"
     CDS             complement(8788..10182)
                     /gene="galP"
                     /locus_tag="ACHK8E_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP0342592.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(10619..11773)
                     /gene="metK"
                     /locus_tag="ACHK8E_000267"
     CDS             complement(10619..11773)
                     /gene="metK"
                     /locus_tag="ACHK8E_000267"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP0342593.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            11829..12080
                     /gene="yqgD"
                     /locus_tag="ACHK8E_000268"
     CDS             11829..12080
                     /gene="yqgD"
                     /locus_tag="ACHK8E_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP0342594.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            12191..12334
                     /gene="yqgG"
                     /locus_tag="ACHK8E_000269"
     CDS             12191..12334
                     /gene="yqgG"
                     /locus_tag="ACHK8E_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP0342595.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            12428..12559
                     /gene="yqgB"
                     /locus_tag="ACHK8E_000270"
     CDS             12428..12559
                     /gene="yqgB"
                     /locus_tag="ACHK8E_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP0342596.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            12568..14544
                     /gene="speA"
                     /locus_tag="ACHK8E_000271"
     CDS             12568..14544
                     /gene="speA"
                     /locus_tag="ACHK8E_000271"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP0342597.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            14690..15421
                     /locus_tag="ACHK8E_000272"
     CDS             14690..15421
                     /locus_tag="ACHK8E_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342598.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            15557..16477
                     /gene="speB"
                     /locus_tag="ACHK8E_000273"
     CDS             15557..16477
                     /gene="speB"
                     /locus_tag="ACHK8E_000273"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP0342599.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(16525..17283)
                     /gene="loiP"
                     /locus_tag="ACHK8E_000274"
                     /gene_synonym="yggG"
     CDS             complement(16525..17283)
                     /gene="loiP"
                     /locus_tag="ACHK8E_000274"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP0342600.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            17561..19552
                     /gene="tkt"
                     /locus_tag="ACHK8E_000275"
     CDS             17561..19552
                     /gene="tkt"
                     /locus_tag="ACHK8E_000275"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0342601.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            complement(19598..20506)
                     /locus_tag="ACHK8E_000276"
     CDS             complement(19598..20506)
                     /locus_tag="ACHK8E_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFP0342602.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            20883..21326
                     /gene="cmtB"
                     /locus_tag="ACHK8E_000277"
     CDS             20883..21326
                     /gene="cmtB"
                     /locus_tag="ACHK8E_000277"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP0342603.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            21354..22742
                     /gene="cmtA"
                     /locus_tag="ACHK8E_000278"
     CDS             21354..22742
                     /gene="cmtA"
                     /locus_tag="ACHK8E_000278"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP0342604.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            22757..24034
                     /gene="yggP"
                     /locus_tag="ACHK8E_000279"
     CDS             22757..24034
                     /gene="yggP"
                     /locus_tag="ACHK8E_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0342605.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            24031..24346
                     /locus_tag="ACHK8E_000280"
                     /pseudo
     CDS             24031..24346
                     /locus_tag="ACHK8E_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            24368..24877
                     /gene="fumE"
                     /locus_tag="ACHK8E_000281"
     CDS             24368..24877
                     /gene="fumE"
                     /locus_tag="ACHK8E_000281"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP0342606.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            24874..25587
                     /gene="yggC"
                     /locus_tag="ACHK8E_000282"
     CDS             24874..25587
                     /gene="yggC"
                     /locus_tag="ACHK8E_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP0342607.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            25872..26891
                     /gene="epd"
                     /locus_tag="ACHK8E_000283"
     CDS             25872..26891
                     /gene="epd"
                     /locus_tag="ACHK8E_000283"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP0342608.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            26941..28104
                     /gene="pgk"
                     /locus_tag="ACHK8E_000284"
     CDS             26941..28104
                     /gene="pgk"
                     /locus_tag="ACHK8E_000284"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP0342609.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            28318..29397
                     /gene="fbaA"
                     /locus_tag="ACHK8E_000285"
     CDS             28318..29397
                     /gene="fbaA"
                     /locus_tag="ACHK8E_000285"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0342610.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            29588..30448
                     /gene="mscS"
                     /locus_tag="ACHK8E_000286"
     CDS             29588..30448
                     /gene="mscS"
                     /locus_tag="ACHK8E_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0342611.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            30587..31222
                     /gene="argO"
                     /locus_tag="ACHK8E_000287"
     CDS             30587..31222
                     /gene="argO"
                     /locus_tag="ACHK8E_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP0342612.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            31315..32055
                     /gene="yggE"
                     /locus_tag="ACHK8E_000288"
     CDS             31315..32055
                     /gene="yggE"
                     /locus_tag="ACHK8E_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP0342613.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            32223..33119
                     /gene="ygfI"
                     /locus_tag="ACHK8E_000289"
     CDS             32223..33119
                     /gene="ygfI"
                     /locus_tag="ACHK8E_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0342614.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(33116..34594)
                     /gene="scpC"
                     /locus_tag="ACHK8E_000290"
     CDS             complement(33116..34594)
                     /gene="scpC"
                     /locus_tag="ACHK8E_000290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP0342615.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(34618..35403)
                     /gene="scpB"
                     /locus_tag="ACHK8E_000291"
     CDS             complement(34618..35403)
                     /gene="scpB"
                     /locus_tag="ACHK8E_000291"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP0342616.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(35414..36409)
                     /gene="meaB"
                     /locus_tag="ACHK8E_000292"
     CDS             complement(35414..36409)
                     /gene="meaB"
                     /locus_tag="ACHK8E_000292"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP0342617.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(36402..38546)
                     /gene="scpA"
                     /locus_tag="ACHK8E_000293"
     CDS             complement(36402..38546)
                     /gene="scpA"
                     /locus_tag="ACHK8E_000293"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP0342618.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSHYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(38750..39643)
                     /gene="argP"
                     /locus_tag="ACHK8E_000294"
     CDS             complement(38750..39643)
                     /gene="argP"
                     /locus_tag="ACHK8E_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP0342619.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            39784..39969
                     /locus_tag="ACHK8E_000295"
     CDS             39784..39969
                     /locus_tag="ACHK8E_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342620.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            40025..40684
                     /gene="rpiA"
                     /locus_tag="ACHK8E_000296"
     CDS             40025..40684
                     /gene="rpiA"
                     /locus_tag="ACHK8E_000296"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP0342621.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            40940..42172
                     /gene="serA"
                     /locus_tag="ACHK8E_000297"
     CDS             40940..42172
                     /gene="serA"
                     /locus_tag="ACHK8E_000297"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0342622.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            42401..42460
                     /gene="ibsC"
                     /locus_tag="ACHK8E_000298"
     CDS             42401..42460
                     /gene="ibsC"
                     /locus_tag="ACHK8E_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP0342623.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(42561..43163)
                     /gene="fau"
                     /locus_tag="ACHK8E_000299"
     CDS             complement(42561..43163)
                     /gene="fau"
                     /locus_tag="ACHK8E_000299"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP0342624.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(43184..43367)
                     /gene="ssrS"
                     /locus_tag="ACHK8E_000300"
     ncRNA           complement(43184..43367)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK8E_000300"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(43409..43738)
                     /gene="zapA"
                     /locus_tag="ACHK8E_000301"
     CDS             complement(43409..43738)
                     /gene="zapA"
                     /locus_tag="ACHK8E_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0342625.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            43906..44484
                     /gene="ygfB"
                     /locus_tag="ACHK8E_000302"
     CDS             43906..44484
                     /gene="ygfB"
                     /locus_tag="ACHK8E_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP0342626.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            44510..45835
                     /gene="pepP"
                     /locus_tag="ACHK8E_000303"
     CDS             44510..45835
                     /gene="pepP"
                     /locus_tag="ACHK8E_000303"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP0342627.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            45832..47010
                     /gene="ubiH"
                     /locus_tag="ACHK8E_000304"
                     /gene_synonym="visB"
     CDS             45832..47010
                     /gene="ubiH"
                     /locus_tag="ACHK8E_000304"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP0342628.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            47033..48235
                     /gene="ubiI"
                     /locus_tag="ACHK8E_000305"
                     /gene_synonym="visC"
     CDS             47033..48235
                     /gene="ubiI"
                     /locus_tag="ACHK8E_000305"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP0342629.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(48309..48392)
                     /locus_tag="ACHK8E_000306"
     CDS             complement(48309..48392)
                     /locus_tag="ACHK8E_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342630.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            48683..49777
                     /gene="gcvT"
                     /locus_tag="ACHK8E_000307"
     CDS             48683..49777
                     /gene="gcvT"
                     /locus_tag="ACHK8E_000307"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP0342631.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            49801..50190
                     /gene="gcvH"
                     /locus_tag="ACHK8E_000308"
     CDS             49801..50190
                     /gene="gcvH"
                     /locus_tag="ACHK8E_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP0342632.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            50309..53182
                     /gene="gcvP"
                     /locus_tag="ACHK8E_000309"
     CDS             50309..53182
                     /gene="gcvP"
                     /locus_tag="ACHK8E_000309"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP0342633.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(53346..54779)
                     /gene="bglA"
                     /locus_tag="ACHK8E_000310"
     CDS             complement(53346..54779)
                     /gene="bglA"
                     /locus_tag="ACHK8E_000310"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP0342634.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            54824..55135
                     /gene="yqfB"
                     /locus_tag="ACHK8E_000311"
     CDS             54824..55135
                     /gene="yqfB"
                     /locus_tag="ACHK8E_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP0342635.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            55299..55958
                     /gene="yqfA"
                     /locus_tag="ACHK8E_000312"
     CDS             55299..55958
                     /gene="yqfA"
                     /locus_tag="ACHK8E_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP0342636.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(56035..57015)
                     /gene="ygfZ"
                     /locus_tag="ACHK8E_000313"
     CDS             complement(56035..57015)
                     /gene="ygfZ"
                     /locus_tag="ACHK8E_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP0342637.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            57268..57534
                     /gene="sdhE"
                     /locus_tag="ACHK8E_000314"
     CDS             57268..57534
                     /gene="sdhE"
                     /locus_tag="ACHK8E_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP0342638.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            57515..57922
                     /gene="ygfX"
                     /locus_tag="ACHK8E_000315"
     CDS             57515..57922
                     /gene="ygfX"
                     /locus_tag="ACHK8E_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP0342639.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(57962..58483)
                     /gene="fldB"
                     /locus_tag="ACHK8E_000316"
     CDS             complement(57962..58483)
                     /gene="fldB"
                     /locus_tag="ACHK8E_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP0342640.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            58595..59491
                     /gene="xerD"
                     /locus_tag="ACHK8E_000317"
     CDS             58595..59491
                     /gene="xerD"
                     /locus_tag="ACHK8E_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP0342641.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            59516..60226
                     /gene="dsbC"
                     /locus_tag="ACHK8E_000318"
     CDS             59516..60226
                     /gene="dsbC"
                     /locus_tag="ACHK8E_000318"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP0342642.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            60232..61965
                     /gene="recJ"
                     /locus_tag="ACHK8E_000319"
     CDS             60232..61965
                     /gene="recJ"
                     /locus_tag="ACHK8E_000319"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP0342643.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            62056..63154
                     /gene="prfB"
                     /locus_tag="ACHK8E_000320"
     CDS             join(62056..62130,62132..63154)
                     /gene="prfB"
                     /locus_tag="ACHK8E_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP0342644.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            63164..64681
                     /gene="lysS"
                     /locus_tag="ACHK8E_000321"
     CDS             63164..64681
                     /gene="lysS"
                     /locus_tag="ACHK8E_000321"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0342645.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(64724..65272)
                     /gene="idi"
                     /locus_tag="ACHK8E_000322"
     CDS             complement(64724..65272)
                     /gene="idi"
                     /locus_tag="ACHK8E_000322"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP0342646.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            65327..65398
                     /locus_tag="ACHK8E_000323"
     CDS             65327..65398
                     /locus_tag="ACHK8E_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342647.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(65395..65520)
                     /gene="yqfG"
                     /locus_tag="ACHK8E_000324"
     CDS             complement(65395..65520)
                     /gene="yqfG"
                     /locus_tag="ACHK8E_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP0342648.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(65522..66970)
                     /gene="uacT"
                     /locus_tag="ACHK8E_000325"
     CDS             complement(65522..66970)
                     /gene="uacT"
                     /locus_tag="ACHK8E_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP0342649.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            67405..69324
                     /gene="ygfT"
                     /locus_tag="ACHK8E_000326"
     CDS             67405..69324
                     /gene="ygfT"
                     /locus_tag="ACHK8E_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP0342650.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            69324..69812
                     /gene="ygfS"
                     /locus_tag="ACHK8E_000327"
     CDS             69324..69812
                     /gene="ygfS"
                     /locus_tag="ACHK8E_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0342651.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(69848..71215)
                     /gene="ghxQ"
                     /locus_tag="ACHK8E_000328"
                     /gene_synonym="ygfQ"
     CDS             complement(69848..71215)
                     /gene="ghxQ"
                     /locus_tag="ACHK8E_000328"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP0342652.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(71251..72567)
                     /gene="guaD"
                     /locus_tag="ACHK8E_000329"
     CDS             complement(71251..72567)
                     /gene="guaD"
                     /locus_tag="ACHK8E_000329"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP0342653.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(72585..73985)
                     /gene="xanQ"
                     /locus_tag="ACHK8E_000330"
     CDS             complement(72585..73985)
                     /gene="xanQ"
                     /locus_tag="ACHK8E_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP0342654.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(74150..77020)
                     /gene="xdhD"
                     /locus_tag="ACHK8E_000331"
     CDS             complement(74150..77020)
                     /gene="xdhD"
                     /locus_tag="ACHK8E_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP0342655.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(77017..77796)
                     /gene="ygfM"
                     /locus_tag="ACHK8E_000332"
     CDS             complement(77017..77796)
                     /gene="ygfM"
                     /locus_tag="ACHK8E_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP0342656.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(77847..79175)
                     /gene="ssnA"
                     /locus_tag="ACHK8E_000333"
     CDS             complement(77847..79175)
                     /gene="ssnA"
                     /locus_tag="ACHK8E_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP0342657.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(79178..82276)
                     /gene="ygfK"
                     /locus_tag="ACHK8E_000334"
     CDS             complement(79178..82276)
                     /gene="ygfK"
                     /locus_tag="ACHK8E_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP0342658.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(82598..83176)
                     /gene="mocA"
                     /locus_tag="ACHK8E_000335"
     CDS             complement(82598..83176)
                     /gene="mocA"
                     /locus_tag="ACHK8E_000335"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP0342659.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            83280..84050
                     /gene="yqeC"
                     /locus_tag="ACHK8E_000336"
     CDS             83280..84050
                     /gene="yqeC"
                     /locus_tag="ACHK8E_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP0342660.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            84098..85723
                     /gene="yqeB"
                     /locus_tag="ACHK8E_000337"
     CDS             84098..85723
                     /gene="yqeB"
                     /locus_tag="ACHK8E_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP0342661.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(85764..86696)
                     /gene="arcC"
                     /locus_tag="ACHK8E_000338"
     CDS             complement(85764..86696)
                     /gene="arcC"
                     /locus_tag="ACHK8E_000338"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0342662.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(86744..88129)
                     /gene="hyuA"
                     /locus_tag="ACHK8E_000339"
     CDS             complement(86744..88129)
                     /gene="hyuA"
                     /locus_tag="ACHK8E_000339"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP0342663.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(88182..89393)
                     /gene="ygeY"
                     /locus_tag="ACHK8E_000340"
     CDS             complement(88182..89393)
                     /gene="ygeY"
                     /locus_tag="ACHK8E_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP0342664.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(89451..90647)
                     /gene="dpaL"
                     /locus_tag="ACHK8E_000341"
     CDS             complement(89451..90647)
                     /gene="dpaL"
                     /locus_tag="ACHK8E_000341"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP0342665.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(90705..91892)
                     /gene="ygeW"
                     /locus_tag="ACHK8E_000342"
     CDS             complement(90705..91892)
                     /gene="ygeW"
                     /locus_tag="ACHK8E_000342"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP0342666.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            92371..94149
                     /gene="ygeV"
                     /locus_tag="ACHK8E_000343"
     CDS             92371..94149
                     /gene="ygeV"
                     /locus_tag="ACHK8E_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP0342667.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(94189..94668)
                     /gene="xdhC"
                     /locus_tag="ACHK8E_000344"
     CDS             complement(94189..94668)
                     /gene="xdhC"
                     /locus_tag="ACHK8E_000344"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP0342668.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(94665..95543)
                     /gene="xdhB"
                     /locus_tag="ACHK8E_000345"
     CDS             complement(94665..95543)
                     /gene="xdhB"
                     /locus_tag="ACHK8E_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP0342669.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(95554..97851)
                     /gene="xdhA"
                     /locus_tag="ACHK8E_000346"
     CDS             complement(95554..97851)
                     /gene="xdhA"
                     /locus_tag="ACHK8E_000346"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP0342670.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            98267..99019
                     /gene="actS"
                     /locus_tag="ACHK8E_000347"
     CDS             98267..99019
                     /gene="actS"
                     /locus_tag="ACHK8E_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP0342671.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            99098..99171
                     /locus_tag="ACHK8E_000348"
     tRNA            99098..99171
                     /locus_tag="ACHK8E_000348"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99130..99132,aa:Gly,seq:ccc)
     gene            99336..100550
                     /locus_tag="ACHK8E_000349"
     CDS             99336..100550
                     /locus_tag="ACHK8E_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0342672.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSDVRKAHKREQASKFGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            100678..101373
                     /locus_tag="ACHK8E_000350"
     CDS             100678..101373
                     /locus_tag="ACHK8E_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342673.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVLCVAYDWGDMRRLTPAEMMDRSNNISMDGT
                     IITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESSVINSEETVLLTYGH
                     KSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILRPTG
                     LQQVGRRKRKTNS"
     gene            101621..101818
                     /locus_tag="ACHK8E_000351"
     CDS             101621..101818
                     /locus_tag="ACHK8E_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0342674.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLINQGRFPKPVKIGPRSVAF
                     IESEVDEWINQRIEASRNNAA"
     gene            101899..102675
                     /locus_tag="ACHK8E_000352"
     CDS             101899..102675
                     /locus_tag="ACHK8E_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0342675.1"
                     /translation="MPVQGQGFVQPEELSQQYFAAIIPVISGNEIIADGINKNALTAP
                     EVFPSGGEPVTTSQAIARFFRKDHDKVVNRIENLCCSAEFLTRNFRRVKFEHKGRVLK
                     AYEVTKDGFVFLTMGFTGKKAAAFKEAYIAQFNRMANWIAERCEAKAEMRSMGDAMKG
                     YIERTGDTQHGHAYNNECTFIARLVLGMHPPVWAKKNGIPSKKVRDHMTADQLALLAY
                     LESRNCALLDMDMPTPERKERLTELAQRWLVRRLGEREHA"
     gene            103295..103690
                     /locus_tag="ACHK8E_000353"
                     /pseudo
     CDS             103295..103690
                     /locus_tag="ACHK8E_000353"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            103721..103909
                     /locus_tag="ACHK8E_000354"
     CDS             103721..103909
                     /locus_tag="ACHK8E_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0342676.1"
                     /translation="MATTPTLVHSQTAFIWRFIIFGASESQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQEASHG"
     gene            103902..104366
                     /locus_tag="ACHK8E_000355"
     CDS             103902..104366
                     /locus_tag="ACHK8E_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0342677.1"
                     /translation="MDKNMLIASSFGLYDAGVAHMATGFSSLNQIADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSYLASLLTPLRLATMFSSVVARLLNQQIGAFHMAVS
                     ARPYFVWRFMQCLTGSIAIFTVTAATEREARARLPHAHLIFVARIRQEVTHA"
     gene            104359..104544
                     /locus_tag="ACHK8E_000356"
     CDS             104359..104544
                     /locus_tag="ACHK8E_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342678.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            104548..104742
                     /locus_tag="ACHK8E_000357"
     CDS             104548..104742
                     /locus_tag="ACHK8E_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342679.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            104809..104922
                     /locus_tag="ACHK8E_000358"
                     /pseudo
     CDS             104809..104922
                     /locus_tag="ACHK8E_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            104928..106751
                     /locus_tag="ACHK8E_000359"
     CDS             104928..106751
                     /locus_tag="ACHK8E_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP0342680.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKERGKGDDATSLALNQMGAS
                     QRGEVLLAHYGGELAINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNA
                     IKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPA
                     EGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGK
                     SVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAIT
                     GGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENER
                     DPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGY
                     LMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMAE
                     YGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            107243..107692
                     /locus_tag="ACHK8E_000360"
     CDS             107243..107692
                     /locus_tag="ACHK8E_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0342681.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADAVARHEKGDLISVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEAQERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            107709..108176
                     /locus_tag="ACHK8E_000361"
     CDS             107709..108176
                     /locus_tag="ACHK8E_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0342682.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKRQRKNRRRINRLAEYWPE
                     LFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSHLHTTRYLKAIIAGGPRY
                     DLNGQPCGEVTEKEKAAASEQLMWRLEQIKQQNIQRQDTGGGESDNRQRGTET"
     gene            108145..108438
                     /locus_tag="ACHK8E_000362"
     CDS             108145..108438
                     /locus_tag="ACHK8E_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0342683.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(108889..109275)
                     /locus_tag="ACHK8E_000363"
     CDS             complement(108889..109275)
                     /locus_tag="ACHK8E_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342684.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVRGEIGNPPFDVSSVAYLLYGMD
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERREGTGDGVRRKVARYGLPGHSVVVRD
                     GDGRNGAIDGECIRINATDVVLVDGQ"
     gene            109360..109662
                     /locus_tag="ACHK8E_000364"
     CDS             109360..109662
                     /locus_tag="ACHK8E_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0342685.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKSDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            109664..109831
                     /locus_tag="ACHK8E_000365"
     CDS             109664..109831
                     /locus_tag="ACHK8E_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0342686.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            110356..110517
                     /locus_tag="ACHK8E_000366"
     CDS             110356..110517
                     /locus_tag="ACHK8E_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342687.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            111015..111407
                     /locus_tag="ACHK8E_000367"
     CDS             111015..111407
                     /locus_tag="ACHK8E_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0342688.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(112238..112612)
                     /locus_tag="ACHK8E_000368"
     CDS             complement(112238..112612)
                     /locus_tag="ACHK8E_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342689.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT        27858 a        28015 c        29838 g        27602 t
ORIGIN      
        1 atatttttgc cgaagttaat taacgggggg gggtaaaaga agtgattaat gcgcccgcag
       61 gcgcattaat gattactcag cgaggaataa ttcaagaagt gagtttaaga ataatttacc
      121 atgctcggtt atctgccagt aatccgcaca ttctgtgaga taaccctgag cgatagcctc
      181 gtctaactga gagcgaataa cttcttcaga aaggccagta tactgactaa actccacgcg
      241 cggcgcggcc tcaagcagac ggaagcgatt cataaagaac tcaaacggtt tatctgcagc
      301 ttcgacatca cgctggcttt ccagatatct tccctgcata aaaccacgcg gatgacgcgt
      361 tttggtggta cgcagaatgc gcccatccgg gaaggtcact ttgccgtgcg cgccgcagcc
      421 aataccgatg tagtcgccaa atcgccagta gttgagattg tgctggcact gataaccggg
      481 tttggcgtaa gcggatgttt catactgctg atagcccgct gcggttaata actgatgccc
      541 ctgttcgaaa atatcccaca gcgcgtcatc gtccggcagt acaggagggc gcgagccaaa
      601 cagcgtatta ggttcgatgg tcagttgata ccaggaaaga tgtggcggat tcagttcaat
      661 agcctggcgc agatcgccta gcgcctcttc cagtgattga tccggcagcc catgcatcaa
      721 atcaaggtta aaactacgta accctaaacc gctcgccagc tttgccgctc gtttcgcttc
      781 ttgcgggcca tgaatacgcc cgagtcgttt cagcttttct tcgctaaaac tctgcacacc
      841 aatagagatg cggttcacac cagcacgctg ataatcgaca aagcgatccg cttctaccgt
      901 gcctgggttc gcttccatcg taatttctgc atccgctgcc agcggcaaac gcgcacgcac
      961 gccgtccagc agcgtttgca tcgccgggcc ggaaagcagg ctcggcgtac caccgccaat
     1021 aaagattgtc tgcacttcac ggccctgagc gtaagccaca tcgttgtcca gatcgttaag
     1081 cagatgctga acataatcgt cgtgcggcac ttctcctttc aacgcgtgag agttgaaatc
     1141 gcagtacggg catttctgca cgcaccacgg gatgtgaatg tagagactca gcggcggtaa
     1201 tttaaccatt acgtaaagcg tccagcagca gtttcaatgc ctgaccacgg tgggaaatgg
     1261 cgctcttttc ttcgcgggtc agttcggcag cggttttccc ttcagaagga acgaagaaaa
     1321 ttggatcata accaaagcca ccagtacccg ctggttcacg agtaatcacg cccggccagc
     1381 taccgtggca caccagcgga gtcggatctt ccgcgtgacg cagatatatc agcacgcagt
     1441 ggaaacgcgc ctgacgttgg tcgtccggta cgtctttcat tgtttccagc agtttttgca
     1501 ggttcttttg atcggtcgcg tcttcaccgg aataacgcgc ggagtaaata cccggcgcgc
     1561 cacccagcgc atctaccgcc agaccggagt cgtcggcaat tgccggtaaa ccggtcactt
     1621 ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa ggtcaggccg gtttcttcag
     1681 cggaatctac gccgaggtct gtttgggcca cgatatcaag accgaagtcg ctaagcagcg
     1741 acgccagctc acgcacttta ccggcattgc cggttgcgag gacaactttt tgcataggat
     1801 acctaattaa ttaacgccgc gatttctggc gggatttgtt gcggattaat gattttaatt
     1861 tgtttgtggc ggccaagttc gcctttttca atcaccacct ggcttttggc aacccggaat
     1921 tgcttaccga gaaacttcac cagatggctg ttggcctggc cgtcaaccgg cggcgcggta
     1981 atggcgactt ttacctcgtc gccatgtaaa ccgacaatag aatcacggct ggctttcggc
     2041 tgaatataga gccgtaaaac cagaccgtca tcattaactg ttacggcact cataacgcca
     2101 tccacagccc cggcagcagc atgtttccgg ttgcctgtaa tacttctgcg acacccatat
     2161 tgatgacata cagcagcaga acgaggatca tcggcgagaa atcaatccca cccattgccg
     2221 gtagcaggcg gcgaatcggg cgcagcagcg gatcggcaag ctgaatcagc acgtattcaa
     2281 tcggactacg accctggctt acccagctca taatcgccat caccagcagg acccagaaaa
     2341 tcagcaggcc gatggttttc agcagaatca gtaaaccggc aatccagatg attggcagga
     2401 aggtcaccac tttaaacagc acgatggctt tgataaaact gagaatatag gcaaccagca
     2461 gcgaggcgct gtcaatgggt cccattgccg gaataacgcg gcgcagtggc ccgataattg
     2521 gctgcgttac cttcactaca aactgtgaga aggggttgta aaaatcacaa tgagcccact
     2581 gcatccagat gcgtaataac agcaccatgg tatacagctc aatgaccgtt gaaagcagga
     2641 aagtcaacgt attcatggcg ttcctttaat tccttatttt ttagagtaat cacgcgcacc
     2701 aaaaattgca gtgccgatac gaaccatcgt gctacctgcc gcaatggcgg cttccatatc
     2761 gtccgacatt cccagagaga gcgtgtcgat atgcgggtag cgcgttttca gtccggcaaa
     2821 tgctacagcc atttggcgtg caacttcaaa ctgccttaca tattctgact caggcgcagg
     2881 gattgccatc agcccgcgca gacgtaaacg cggtagttca gcgaccgcag ctgccagctc
     2941 gtccagttct gccagttgaa tcccggactt actgttttca tcactaatat taatttgaat
     3001 cagtacgtca agagggggaa gttctgccgg gcgctgatcg ttgaggcggg tagcaatgcg
     3061 caaacggtcg atggtatgac accagtcgaa atgctctgcc accaggcggc ttttattaga
     3121 ctgcaacggg ccaataaaat gccattctaa tcctgttacg cccagttcct gaaagtggcg
     3181 aattttatct accccttcct gaacgtagtt ttcaccaaat tgacgctgcc cggcatcaat
     3241 ggcttctgcg atggcgctcg caggttttgt tttactgact gcaagcagcg taatttcttc
     3301 tggagaacgg ccgcaacgcg ttgcagccgc tgagattttg tcccggacct gtgccaggtt
     3361 atgcgcaata tcgttcattt tccgaggatc ttagtatgaa tatggaagaa attgtggccc
     3421 ttagtgtaaa gcataacgtc tcggatctac acctgtgcag cgcctggccc gcacgatggc
     3481 gcattcgcgg gcgaatggaa gctgcgccgt ttgatgcgcc ggacgtcgaa gagctactgc
     3541 gggagtggct ggatgacgat cagcggacaa tattgctgga gaatggtcag ttggattttg
     3601 ctgtgtcgct ggcggaaaac cagcggttgc gtggcagtgc gttcgcgcaa cggcaaggta
     3661 tttctctggc attacggttg ttaccttcgc actgtccaca gctcgaacag cttggtgcgc
     3721 caccggtatt gccggaatta ctcaagagcg agaatggcct gattctggtg acgggggcga
     3781 cggggagcgg caaatctacc acgctggcgg cgatggttgg ctatctcaat caacatgccg
     3841 atgcgcatat tctgacgctg gaagatcctg tcgaatatct ctatgcaagc cagcgatgtt
     3901 tgatccagca gcgggaaatc ggtttgcatt gtatgacgtt cgcatcggga ttgcgggctg
     3961 cattgcggga agatcctgat gtgattttgc tcggagagct gcgtgacagc gagacaatcc
     4021 gtctggcgct gacggcggca gaaaccgggc atttggtgct ggcaacatta catacgcgtg
     4081 gtgcggcgca ggcagttgag cgactggtag attcatttcc ggcgcaggaa aaagatcccg
     4141 tgcgtaatca actggcaggt agtttacggg cggtgttgtc acaaaagctg gaagtggata
     4201 aacaggaagg acgcgtggcg ttgtttgaat tgctgattaa cacacccgcg gtggggaatt
     4261 tgattcgtga agggaaaacc caccagttac cgcatgttat tcaaaccggg cagcaggtgg
     4321 ggatgataac gtttcagcag agttatcagc agcgggtgaa agaagggcgc ttgtgacgtt
     4381 gctgtgtctg atacgaattg taaatatgcc cgatgcgcaa gcttatcggg cctacaaggg
     4441 aagcgtaact ttgaattcgc actcactgta ggttggataa ggcgtttacg ccgcatccaa
     4501 catgaaacaa cgagcacatt gtcagaaatc tgtgcgctag taaatcgcct taatatccct
     4561 gctcgaaata gctttcgaga ataataaccg cagaggcaga gtcgactttg cctttgttga
     4621 gcgcccgata gccgccctgt tcaaacagac cggaacgtgc ctccacggtg ctaagacgct
     4681 cgtcatggag ctttacttca acaccgaaac gaccatgaat acggttggca aatttacgcg
     4741 ctctggcggt taacggctgt tcggtgccgt ccatattcag cggcaaaccg acgatgattt
     4801 cgtcaggctg ccactctttc agtaaacgct cgataatgtt ccagtccggc gtaccgtcct
     4861 gtgctttaat tgcaggcaat gggcgagcgg tgccggtaat gcgttggccg accgctacac
     4921 caatgctttt ggtaccgaag tcgaaggcga gtaaggttcc actcatcagg cgtgccctgc
     4981 cacaccaggc atggtgagaa tatccacacc aatcagtttt gccgcctcgc gccagcggtc
     5041 ggcaatcggt gttttgaaca gaatattcag atccgccggg gccgttagcc acgcgttatc
     5101 gagaatttct tgttccagtt gacctttctc ccaggaggca tagcccagag ccaccaatac
     5161 gtcagacggt tgtttatcgg tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac
     5221 cgtgttgtct gaaatgcgaa tgctggaagc aaaattagag ggcggagtat gcaaaataaa
     5281 cccgcgatct tcagccagcg gaccaccgag cataaccggt ttatccagac ggattgattc
     5341 atcacgcggc tccggcgtga tcttcagctt ttccagaatc ccttcaattt tgagattttc
     5401 cagcggcttg ttgacgatga tccccattgc accattggta ttatgttcgc aaatgtagac
     5461 cacggaacga cggaaaatcg gatcctggag agcaggcatg gcaataagaa agtgatgctg
     5521 taaattcatt gtcagaggtt ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg
     5581 tggaaagaag ggcgctgtca ctcagagtct caacgagatc cttctcgcta aggtgggtta
     5641 ctgctgctgt aaacgtgctt cgatggcatc cattaacatt ccggtgatcg acaccggaaa
     5701 ctctgcttca atctcacgaa tacaggttgg gctggtgacg ttaatttcag tcagacggtc
     5761 gccaatgata tccagaccaa caaaaatcag ccctttttct ttcagcgtcg gcccgatctg
     5821 acgggcgatt ttccagtcac tttccgtcag cggacgaggt tcaccgcgac caccggcagc
     5881 cagattgcca cgggtttcgc ccccctgcgg aatacgcgcc aggcagtacg gtaccggctc
     5941 gccatccacc accagcacgc gtttgtcgcc atctttaatg gctggcaggt aattttgcgc
     6001 catgcagtag cgagtgccat gctcagtcag ggtttcggca atcacgccga ggtttggatc
     6061 gccttctttc acgcggaaaa tcgacgcgcc gcccataccg tccagcggct taagaatgat
     6121 gtcgctgtgt ttctcccaga acgcttttag ctgcgcttta ttgcgcgtaa ccagcgtttc
     6181 tggcgttaag tcagagaacc aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg
     6241 cggcttgtta acgatcagcg tccctttctc ttcggcacgt tccagaatat aggtcgcgta
     6301 gataaactcg gtatcaaacg gcgggtcttt acgcatcagg atcacatcga gatcggccag
     6361 cggcagatcc tgttcaccga cgaatgaaaa ccactcttcg tagttctgct tcacgttcag
     6421 cgtgcgggta tgggcgcggg cttcgccatt gatcagatac agatcgccca tctccatata
     6481 gtgaagttcg taaccacgac gctgtgcttc cagcaacata gcgaaactgg aatctttctt
     6541 gatgttgatg tttgcgatgg ggtccatcac gatgccgagc ttgatcatta ttcttctccg
     6601 ttagcccaaa tcgccaaatc gtacttgtag cgcggtaatg gcggtgagcg cagttgtctc
     6661 tgtacgcaaa acgcgaggtc ccaacaggat atcagtaaat tgatagcggg cagtcatggc
     6721 aatttcatct gccgataaac cgccttccgg gccaatcagc aggcggacac gttcaaccgg
     6781 taacggcaac gtattgatgc tgttactggc gcgcgggtga agattcagtt tcagtccttc
     6841 atcctgctct gcacaccagg cttccagatc catcgctgga cggatttccg gcacccggtt
     6901 acgaccacac tgctcacagg cagcaattgc aatcttctgc cactgctgga gcttcttgtt
     6961 cagacgttca ctatccagtt taacgccgca gcgctcagaa aaaagtggcg taatgaggct
     7021 tacaccgagt tcgatcgatt tctggatcgt aaattccatt ttttcaccac gcgacatcac
     7081 ctgaccgagg tgaatatgca gcggagattc gcgatcgtcg atctggcctt ccagtacctt
     7141 cacttccacg ctttttttgc tggcgctggt aatttcggcg tcaaagacct ggttgctacc
     7201 gtcaaacaat tgcaacgcct gccccggccc catacgcagt acgcgcccga tatggttggc
     7261 ggcatcttcg caaagcgcga tgtgagaatg gctggtcagt ggttcaggat gataaatgcg
     7321 ggggatacgc atagttaaaa atccgcgtcg tctccccacg cggttgtacg cgtggggtag
     7381 gggttaacaa aaagaatccc gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt
     7441 gcacatacgg gttatgattg ccctgcacct tcgcgatgcg ttcatcgcgc tcgcactccc
     7501 agtcggtaac cggatacatc ttgtcccatg cgttgaacag ttgcgtttgc tggcgagaga
     7561 gtgtcaggtt gtattggtca cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac
     7621 gcgctggtgg ttcggcggct ttttctttga aatcgacctt catggcgcat tgaccgtact
     7681 ggccttcgcc gccattccac tggctgtaca taaagttgcc gcgatcgcca ttcacctcac
     7741 cgactgacgg ctgcaggtta tgcatatcgc tttccatctt gcgatagacc ggatctttgg
     7801 cgcagttttt acgtccaccg tcctgccagc actggcgctg gtgaccgaat tgccaggcgg
     7861 gaacgacgtg ttcccactct acgcggctgg cgcggttttc atttttgcgc acctgatagc
     7921 cgcacgattg cagatcaaca acgccttttt tgccctgcca gttaatttta catccgcaat
     7981 aaaacgtacc gggcgcatca gcgtggactt ttaccgccgc ggctttcgcc tgagaaaaac
     8041 tattgatacc ttcggccaac gccgggccgg aaaatgctgc gctcagtacc accgcagcaa
     8101 tagacaaata acggtacatc acttactccg tgttaaaacg agccagcaac gtagcgaaag
     8161 ttgttttggt atgcaatcag ccagatcagg aaagttcctg atagttcagg tggttatttc
     8221 gcaaccagct gttcaccgca gtgaacacag cgatagacgg cctcgccacg aactacgcga
     8281 ttatggcggc gtacggtaag ctgatgctcc tggcacttgc agcggtaggg gaaggtgttg
     8341 cgacgcacgg attgcagttc gaactgatgc gtacgacggg cgggaacgcc cagcacgctt
     8401 tccatcatcc acttccactc tttgccatgt ggcgctacgc ggccgaaatg tttccatacc
     8461 agcaaatgtg ccagttcgtg cggtaccact tcttcaataa aagcttcact gttttccaac
     8521 agcaaaacgg gattgaggcg gatttcatag ctttccagcc aggctgttcc ggcggaggtt
     8581 ccgcgctggg tgtaagagag ttttggctcc gggtagttac gccctagctt caggttggcc
     8641 tgggcgagtt tttcccgcag gcggcgcata acggcctgct ggatggcgat agggagacgg
     8701 gatgttttca tagcggcaga ggatagagcg aagaaaagat gactgcaaga ggtggcttcc
     8761 tccgcgacgg gaggaagcct gaggagatta atcgtgagcg ccgatttcgc gcagtttacg
     8821 acctttcatc agattacgtt caatatgttc cagcgaaacg tgtttggttt ccggtaccag
     8881 ccacaatgtc agcaagataa acagtacgtt cagagccgca tacacccaga aggtgttggc
     8941 gttacccagc gtgttgagca tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca
     9001 gttggtggcg gtggagcagg tgatgccaaa atcgcggcct ttcagcggct gaatttcgga
     9061 gcacagtacc caaatcagcg gaccggcact catggcaaaa ccgacaataa acatcagcag
     9121 catggcgatg gcgaaatact gcgccgacgg agagtggata ccgatatgca tcattgtacc
     9181 gagtacgccc atgccagcag ccatcaccag gaagcccagc gttagcgttg gtttacgtcc
     9241 ccagcggtca acaaggccga ttgcgataaa ggtggcgagt acgttggtca ggccgacgat
     9301 caccgtcccc cacatttgct cggtggtgtt ggtataaccc gccagttcga agattttcgg
     9361 cgcgtaatac atgatgacgt tcatcccggt gaattgctgc attacctgca acagtacgcc
     9421 aaggaacacc gcgcggcgga agttgctgtt ctctttaaac agcgcccagc cactctgttt
     9481 aacctgcaaa ctttcacgga tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg
     9541 caggcgtagc agcacgcgtt cggcatcaac aaaacggcgt ttggcggcaa accaacgagg
     9601 actgtctggc aggaagaaga caccaatcag cagcaaaatt gccgggatga taatcacacc
     9661 cagcatccag cgccatgcac cggtgtagct gaaggcggta tcagaaagat aagcaccgag
     9721 gatcccgata gtgatcatca actgatacat cgagatcatg ctgccgcgaa ttttttccgg
     9781 cgcaatttca gagaggtaca gcggtgcggt ataagaagcc acacccaccg ccaaccccag
     9841 cagaacgcgg gaaagaatca gtacttcaac gtttggcgca gccgcagaga acagtgaacc
     9901 ggcaacaaac agaattgcgc cgatcatcag gctctttttg cgcccgagtt taaaggagag
     9961 ccagccactg cccaccgcac cgactgccgc accgaacatc atggagctta cgacccattc
    10021 ttgcgtgtgc gaagtaatct ggaattcatc tgcaataaac ggcagtgcgc cagcaattac
    10081 accgatatcc aggccaaaga gtaatcccgc cagagcggca aggaagcaga cgaaaaacgt
    10141 cattgccttg tttgaccgcc cctgtttttt agcgtcaggc atgatgccct ccaatatggt
    10201 tattttttat tgtgaattaa gataggtgag tacgacgtaa aaagatgtga agcaaatcac
    10261 atcagtgtaa tcgcttacac taacatttat cttggttatg atttaagtgc atgaaaataa
    10321 ttgagttata tcatcataga ttaaacgctg ttatctgcaa ttaagacttt actgaaaaga
    10381 aatgtaacaa ctgtgaaagc tgaataataa tatgaaaata ttacaaatat ttggctggcg
    10441 tggttggtgt aatcggtttc agtagtgatg gtaatttgcg gtaaaacgtg ttgaggagga
    10501 atgatgttat tgtgcgggcg gtttttttgc cggatgcggc gtgaatgcct gtccaccgcg
    10561 gtgaggtaaa agaaaaaggc cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt
    10621 acttcagacc ggcagcatca cgcagcagct gcgctttgtc ggttttttcc cacgggaaat
    10681 gttcacgacc aaagtgaccg tatgctgcgg tttctttgta gatcgggtgc agcagatcca
    10741 gcatctgaat cagaccgtat gggcgcaggt cgaagaattc acgtaccagc agagtcagtt
    10801 gttcagaagg cactttctca gtaccgaaag tttctaccat gatggaagtc ggttcagcca
    10861 cgccgattgc gtaggaaacc tgaatttcac aacgatcggc caggccagca gccacgatgt
    10921 ttttcgcgac ataacgtgct gcgtacgctg cggaacggtc cacttttgat ggatctttac
    10981 cggagaatgc accgccaccg tgacgtgcca tgccgccgta ggtatcaacg ataattttac
    11041 gaccagtcag accgcagtca cccatcgggc caccgataac aaaacgaccg gtcgggttga
    11101 tgaagaattt ggtggcagaa gtcagccatt cagccggcag aatcggcttg atgatctctt
    11161 ccattaccgc ttcttgcagc gatttctggt cgatttcttc agagtgctga gtggaaagca
    11221 cgacagcatc gataccaacg attttgccgt cgtcatactg gaaagtcacc tggcttttcg
    11281 cgtccgggcg cagccacggc agagtgccgt ttttacgcac ttcagcctga cgctgtacca
    11341 gacggtgtgc ataggtgata ggcgctggca tcagcacgtc ggtttcattg gttgcgtagc
    11401 caaacatcag accctggtca cccgcgccct gttccagcgg atcggcacgg tcaacgccct
    11461 ggttgatgtc aggagactgt ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa
    11521 agcccatgtc ggaatgcaca tagccaattt cgcgaacggt gttacgggtg atctcttcga
    11581 tgtctaccca ggcgctggtg gtgatttcgc cgccaactaa aaccatgccg gtttttacgt
    11641 aggtttcgca agcaacgcgt gctttcggat cctgttcgag gatcgcgtct aaaacggcat
    11701 cagaaatttg gtcagcaatt ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa
    11761 ggtgttttgc catatttaat atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg
    11821 gattttctgt ggtagcggat cctaccacga ctctgcaggt taaaaacact ggcagtctga
    11881 gtgttaatcg gtatggatgg attaacatct ggatggctat tttaggtcaa ttcttcaccc
    11941 gatttccact tttttttgaa tcgtgtctca ttctgttaaa aacgtggctg gaaatttttc
    12001 ctgacaatgc tggcattctg cgtatttatc ttttgcaatt ttctgccatt gtggggtata
    12061 aaacgcggcg cgcggcttaa ataaaaagca cacgacgttt ctttcgtgtt gccacttcca
    12121 gccgggttga aatcagagtt ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg
    12181 atacgaaata atgaaccgtt gtctgctgct taacctgtct caccgttctg gtgaagattc
    12241 gttccccgca ctctgcatct ctgctttgca tacctgccga tgttataccc atctcggcgc
    12301 ttctcaggat tcaagagctg gttacagtta ctgaggactg aacaagggcg ctcttgtaaa
    12361 aacaagagtt ttctcgtggt ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa
    12421 taatgttatg aaaaagaaac cggttgcgca gttggagcgt cagcattcac tgctggaaaa
    12481 tccatgtgct tatgggttgt tatcgcagtt ccaggctgcg atagtcgtta actgttttac
    12541 acttaataaa ataatttgag gttcgctatg tctgacgaca tgtctatggg tttgccttcg
    12601 tcagcgggcg aacacggtgt actacgctcc atgcaggagg ttgcaatgag ctcccaggaa
    12661 gccagcaaga tgctgcgtac ttacaatatt gcctggtggg gcaataacta ctatgacgtt
    12721 aacgagctgg gccacattag cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat
    12781 ctcgcgcagt tagtgaaaac tcgtgaagca cagggccagc gtctgcctgc actgttctgt
    12841 ttcccacaga tcctgcagca ccgtttgcgt tctattaacg ccgcgttcaa acgtgcgcgg
    12901 gaatcctacg gctataacgg cgattacttc ctcgtttatc cgatcaaagt taaccagcac
    12961 cgccgcgtga ttgagtccct gattcattcg ggcgaaccgc tgggcctgga agccggttcc
    13021 aaagcggagt tgatggcggt gctggcacat gctggcatga cccgtagcgt catcgtctgc
    13081 aacggttata aggaccgcga atatatccgc ctggcattaa ttggcgagaa gatgggccac
    13141 aaggtctatc tggtcattga gaagatgtca gaaattgcca ttgtgctgga tgaagcagaa
    13201 cgtctgaatg tcgttcctcg tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt
    13261 aaatggcagt cctctggcgg ggaaaaatcg aagttcggcc tggcggcgac tcaggtactg
    13321 caactggttg aaacgctgcg tgaagccggg cgtctcgaca gcctgcaact gctgcacttc
    13381 cacctcggtt cgcagatggc gaatattcgc gatatcgcga caggcgttcg tgaatccgcg
    13441 cgtttctatg tggaactgca caagctgggc gtcaatattc agtgcttcga cgttggcggc
    13501 ggtctgggcg tggactatga aggcacccgt tcgcagtccg actgttcggt gaactacggc
    13561 ctcaatgaat acgccaacaa catcatctgg gcgattggtg atgcgtgtga agaaaatggt
    13621 ctgccacatc cgacggtaat caccgagtcg ggtcgtgcgg tgactgcgca tcacaccgtg
    13681 ctggtgtcga atatcatcgg cgtggaacgt aacgaataca cggtgccgac cgcgcctgca
    13741 gaagacgcac cgcgcgccct gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg
    13801 ggaactcgtc gttctctgcg tgaatggtta cacgacagcc agatggatct gcacgatatt
    13861 catatcggct actcttccgg cacctttagc ctgcaagaac gtgcatgggc agaacaactc
    13921 tatctgagca tgtgccatga agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt
    13981 ccgattatcg acgagctgca ggaacgtatg gctgacaaaa tgtacgtcaa cttctcgctg
    14041 ttccagtcga tgccggacgc atgggggatc gaccagttgt tcccggttct gccgctggaa
    14101 gggttggatc aagtgccgga gcgccgcgct gtgctgctgg atattacctg tgattctgac
    14161 ggtgctattg atcactacat cgatggtgat ggtattgcca cgacaatgcc aatgccggag
    14221 tacgatccag agaatccgcc gatgctcggt ttcttcatgg tcggcgcata tcaggagatc
    14281 ctcggcaaca tgcacaacct gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct
    14341 gacggcagcg tagaagtaga actgtctgac gaaggcgata ccgtggcgga tatgctgcaa
    14401 tatgtacagc tcgatccgaa aacgttgtta acccagttcc gtgatcaagt gaagaaaacc
    14461 gatcttgatg ctgaactgca acaacagttc cttgaagagt tcgaggcagg tttgtacggt
    14521 tatacctatc ttgaagatga ataattaaga ttattccgct ggcgggtcga tttatttacg
    14581 taaatcggcc cgctttcttt ttatatattc ttaaaaataa tttagctgct tttcttatta
    14641 aataatcccc ctacaatcgc tgcattaatt aaatgcagag ggaacggtta tgaaaaaatg
    14701 gaaagtacgt agcgcgttga tagcattagc cgtgctgctg gccggttgtt ccagtaatgc
    14761 gcagtataat agcagtgcat caggcaatgt cggcaccgcc tggggtgggg atgtacactc
    14821 atcggttcag ggcgtatctg ctgaacgagt ctggcgcgat ccggcagaga tgatcgttat
    14881 tagttattcg actactgttc cttccggcta tgaccgcgtt tatagcattc gtattaacga
    14941 actggaatat gctattcgcg atggtaattt taattctcta cctattacgc gggtttatga
    15001 ttcatccaat aatgaaccac gctatattgt ccatgccaga gtcggaatga attatcaatt
    15061 atatatccgt aattacagtc gtaataccaa ctacgagatc gttgctacgg ttgatggcct
    15121 ggatgtgctg aacggcaagc aaggttcgct gaataacaac ggctatattg tgaatgccgg
    15181 agattcgtta gcgattaaag gattccgcaa agataaacat accgaagcgg cttttcagtt
    15241 cgccaacgtt gctgattcct acgcagccaa ttcggcgcaa ggcgatgtgc gtaataccgg
    15301 tgtaatcggc tttgccgcct ttgaattaca aggcccggcg caaaacgcgc tgccgccgtg
    15361 ctctggacaa gcctttccgg cagataacaa tggctatgcg ccgccaccgt gtcggaaata
    15421 gcaacctcgc ttgatgcctc gcccatttaa ggcgataatt agcacaaaat tgtgtttcgc
    15481 aaatccaacc cttcctcgtc gggcctaacg acgcggaagg gtttttttat atcgactttg
    15541 taataggagt ccatccatga gcaccttagg tcatcaatac gataactcac tggtttccaa
    15601 cgcctttggt tttttacgcc tgccgatgaa cttccagcca tatgacagcg atgctgactg
    15661 ggtgattact ggcgtgccgt tcgatatggc cacttctggt cgtgcgggtg gtcgccacgg
    15721 tccggcagcg atccgtcagg tttcgacgaa tctggcctgg gaacacaatc gcttcccgtg
    15781 gaatttcgac atgcgtgagc gcctgaacgt cgtggactgc ggcgatctgg tatatgcctt
    15841 cggagatgcc cgtgagatga gcgagaagct gcaggcgcac gccgagaagc tgctggctgc
    15901 cggtaagcgt atgctctcct tcggtggtga ccactttgtt acgctgccgc tgctgcgtgc
    15961 tcatgcgaag catttcggca aaatggcgct ggtacacttt gacgcccaca ccgacaccta
    16021 tgcgaacggt tgtgaatttg accacggcac catgttctat accgcgccga aagaaggtct
    16081 gatcgacccg aatcattccg tgcagattgg tattcgtact gagttcgata aagacaacgg
    16141 ctttaccgtg ctggacgcct gccaggtgaa cgatcgcagc gtggatgacg ttatcgccca
    16201 agtgaaacag attgtgggtg atatgccggt ttacctgacc tttgatatcg actgcctgga
    16261 tcctgcattt gcgccgggaa ccggtacgcc agtaattggc ggcctgacct ccgatcgcgc
    16321 tattaaactg gtacgcggcc tgaaagatct caacattgtt gggatggacg tagtggaagt
    16381 ggctccggca tacgatcagt cggaaatcac cgctctggct gcggcgacgc tggcgctgga
    16441 gatgctgtat attcaggcag cgaaaaaggg cgagtaagcg ccggataaga tgcgttatgc
    16501 gtcaaatccg gcgtaataaa atgcttactt aactccatcc gcgctcatac gatcgcgaat
    16561 atgctgggcg cgttcggcgg atgctggatg gtcgtcaaac attgagcttt gacggccttc
    16621 ttccagcttc gccagttttt caaaactggt ggcaagacct gccgggctga tgccgcgttg
    16681 gcgcagaaga tcgtaagaat aatcatcggc ttctgcttcc tggcgctggg agaattgcga
    16741 attaactaat ttctcgccca gatcaccgag ttgtgattga gataaactgc cgacaatccc
    16801 gcccgcagag gccgcagcta ctcgcacggc atttgtacca agtgccacct gcatcccttt
    16861 cttcacatgg cctaacgcca cgtgccccat ttcgtgaccg atcaccgctt cgacttcgtt
    16921 atccgtcatc atatccatca gcccgctata gacgcggata cagccgttag ccattgcaaa
    16981 ggcgttcaca tccttcgcca tatacacttt gtaattcacc ggctgaccgt tgatattgtt
    17041 gcctagcgca ttggcaatag ttgtcagacg tttagcgtat tcgctattgg ctggcgcaat
    17101 cgtcgccttg ctgtccatct cctgacatgc ctgatcgctc agggttttca cctgcgcatc
    17161 actcaaactg taagcctgaa aagcttccgc tcctgatgag agcagtccgt tggagtccat
    17221 attctggcaa ccggtcagta ccgttgccac gctcattgct accaataagg cgcgaatttt
    17281 catgttttct cttccgcaca aggtcaggat tattctaaaa atgcgccgtt tgcaggtgaa
    17341 tcgacgctca gtctcagtat aaggaaaagc gcagcggacg ggcgagcaga ttgcgcaaca
    17401 tgcgagcatg atccagagat ttctgaagcg gcaaaaggat gttccatgta catgacgcgc
    17461 ggcttgcggt aaattgttgg caaattttcc ggcgtagccc aaaacgcgct gtcgtcaagt
    17521 cgttaagggc gtgcccttca tcatccgatc tggagtcaaa atgtcctcac gtaaagagct
    17581 tgccaatgct attcgtgcgc tgagcatgga cgcagtacag aaagccaaat ccggtcaccc
    17641 gggcgccccg atgggtatgg ctgacattgc cgaagtcctg tggcgtgatt tcctgaaaca
    17701 caacccgcag aatccgtcct gggctgaccg tgaccgcttc gtgctgtcca acggccacgg
    17761 ctccatgctg atctacagcc tgctgcacct caccggttac gatctgccga tggaagaact
    17821 gaaaaacttc cgtcagctgc actctaaaac tccgggccat ccggaagtgg gttacaccgc
    17881 tggcgtggaa accactacag gtccgctggg tcagggtatt gccaacgcag tcggtatggc
    17941 gattgcagaa aaaacgctgg cggcgcagtt taaccgtccg ggccacgaca tcgtcgacca
    18001 ctacacctac gccttcatgg gcgacggctg catgatggaa ggcatctccc acgaagtttg
    18061 ctctctggcg ggtacgctga agctgggtaa actgattgcg ttctacgatg acaacggtat
    18121 ttctatcgat ggtcacgttg aaggctggtt taccgacgac accgcaatgc gtttcgaagc
    18181 ttacggctgg cacgttattc gcgacatcga cggtcatgac gcggcatcca tcaaacgcgc
    18241 agtagaagaa gcgcgcgcag tgactgacaa accgtccctg ctgatgtgca aaaccatcat
    18301 cggtttcggt tccccgaaca aagccggtac gcacgactcc cacggtgcgc cgctgggcga
    18361 cgctgaaatt gccctgaccc gcgaacagct gggctggaaa tatgcaccgt tcgaaatccc
    18421 gtctgaaatc tatgctcagt gggatgcgaa agaagcaggc caggcgaaag aatccgcatg
    18481 gaacgagaaa ttcgcggctt acgccaaagc ttatccgcag gaagccgctg aatttacccg
    18541 ccgtatgaaa ggcgaaatgc cgtctgactt cgatgccaaa gcgaaagaat tcatcgctaa
    18601 actgcaggct aatccggcga aaatcgccag ccgtaaagcg tctcagaatg ctatcgaagc
    18661 tttcggcccg ctgttgccgg aattcctcgg cggctctgct gacctggcac cgtctaacct
    18721 gaccctgtgg tctggttcta aagcaatcaa cgaagatgct gcgggtaact acatccacta
    18781 cggtgttcgc gagttcggta tgaccgcgat tgctaacggt atctccttgc acggtggttt
    18841 cctgccgtac acctccacct tcctgatgtt cgtggaatac gcacgtaacg ccgtacgtat
    18901 ggctgcgctg atgaaacagc gtcaggtgat ggtttacacc cacgactcca tcggtctggg
    18961 cgaagatggc ccgactcacc agccggtaga gcaggtcgct tctctgcgcg tgaccccgaa
    19021 catgtccaca tggcgtccat gtgaccaggt tgaatccgcg gtcgcgtgga aatacggcgt
    19081 tgagcgtcag gacggcccga ccgcgctgat cctctcccgt cagaacctgg cgcagcagga
    19141 gcgtactgaa gagcagctgg caaacatcgc gcgcggtggt tatgtgctga aagactgtgc
    19201 cggtcagccg gaactgatct tcatcgcgac cggttcggaa gttgaactgg ctgttgccgc
    19261 ctacgaaaaa ctgactgccg aaggcgtgaa ggcgcgcgtg gtttccatgc cgtctaccga
    19321 cgcgttcgac aagcaggatg cggcttaccg tgaatccgta ctgccgaaag cggttaccgc
    19381 tcgtgttgcg gtggaagcgg gtattgctga ctactggtac aagtatgttg gcctgaacgg
    19441 tgctatcgtc ggcatgacca ccttcggtga atctgctcct gcagagcaac tgtttgaaga
    19501 gttcggtttc accgttgaca acgttgttgc gaaagcgaaa gcgctgctgt aatcggtaag
    19561 atgctttgtg agaagggccg cgaaagcggc ccttttttta cttagtagag ataaacggaa
    19621 ctgtactgcc ggggatcatg gtatcgggta gcttaccgtc ccaacgctca gcagtggtga
    19681 gggcaacaag tccgggatta tcccgcagag cttcaccgcg caggcggatc gcttctgctt
    19741 cagccgcact ttttaaacga atagtttcag cctctgccgc gcctcttacg cgaatagtct
    19801 cagcttctgc tttggctgcc gccagtttac tgtctgcttc tgcctgggcc tgagttaccg
    19861 ctatttgcgc ctggattttc tctgtttcca gattttgctt acgtgtggca atggcgactt
    19921 ctgctttcat tcgatcttca atcgattttt cataagcgtc agaaaaatca atattttcaa
    19981 tctggacacc atcaatcacc accgggccaa caacggcttt acgcattgca ttttgtagat
    20041 cctgaaccag ttttgtacga tcctgtacgg cgctgatggc ggtatattgg ccgaaaacgt
    20101 tctccagttg agttggcaac tgacggacaa tcagacgatc ttttaatgct tcaatggtgt
    20161 tgtaggtggt ataaaccgcg cctgcttctg aaggtttaat gtggaagctg actgatactg
    20221 tcatttgtgc cggttgctgg tcgcggctgt aggcctgtaa tccctgataa accacagcct
    20281 gattacgcgt ggaaattttc tccacgcttt ccataaaagg gatcttaaaa cccagacccg
    20341 gctcggcaac ttttacgatt tttccgtagc gcagcaaaat gccgcgttca ccttcattaa
    20401 cggtgtaata cgagagaaat ggcagcacaa ccacggcaag cacgccaatg acgatagcaa
    20461 tagatttttg tgggcgaaat gaggttattg aaacaggtgc tttcatatga atcttccttg
    20521 agtaaagtgg ggaatatgtc acatgaggtg aggagaaaat agccgacagg tgagaattca
    20581 gaatctataa aggggtatag cgaaaaaaca ttgcattaga aaagtatgct ttcggacatt
    20641 caataaataa atggcgatat gtatcacaaa tacaacaaca atcaaaatgt tgattttgtg
    20701 ataaacataa caaatacaaa gttgacttta aaaatatcaa tttcatgttg ttgttattta
    20761 aagttttatt atttttgttc atggggtttt tcttctcgcg tacccaattg ctttcattaa
    20821 accgcacgag tattttattt ttaccgccgg acggtgccct aaaaataaaa taaggattat
    20881 ttatgcggct tattgattat tttccagaat catcaatctc agttatacat tcagcaaaag
    20941 attggcagga agctatcgat ttctcgatgg catcattgct ggataaaaac tatatcagcg
    21001 agaattacat tcaggcaatt aaagattcca ccattaacaa tggcccttat tatattctcg
    21061 caccaggcgt ggcaatgcct catgctcgac cggaatgtgg ggcgcttaaa accgggatgt
    21121 ctttaacatt acttaaacaa ggcgtttatt ttccggggaa tgacgaaccg ataaaattac
    21181 tcatcgggct ttctgctgcc gatgccgatt cgcacattgg cgctattcag gcgttaagtg
    21241 aattattgtg tgaagaagaa atactcaacc aattattaac agcagcatca gaaaaacaat
    21301 tagcggacat aatcagccgc ggataattac tctctcctct acaggataat attatggaaa
    21361 acaagtctgc tcgtgcaaag gtccaggctt ttgggggctt tttgactgca atggtcatcc
    21421 ccaatatcgg cgcttttata gcctggggtt ttataacggc gttatttatt cccaccggct
    21481 ggctgcctaa cgaacatttc gccaaaattg tcggcccgat gattacctat ttattgcccg
    21541 tgatgattgg ttctaccggt ggtcatctgg tcggcggtaa acgcggcgcg gtcatgggcg
    21601 gaataggtac tattggtgtg atcgtcggcg cagagatccc gatgttcctt ggctcaatga
    21661 ttatggggcc gctcggtggt ctggtgatta aatatatcga caaggcgctg gaaaagcgga
    21721 tccctgctgg ttttgagatg gttatcaata acttctcatt aggtattgcg gggatgctcc
    21781 tttgtctgct gggttttgaa gttatcggcc cggcggtgtt aattgccaat actttcgtca
    21841 aagagtgtat tgaggcgttg gtacatgcgg gttatctgcc tttgctgtca gtcatcaatg
    21901 aaccggcgaa agtgcttttc ctcaataacg cgatagatca gggcgtctat tacccgttgg
    21961 ggatgcaaca ggcttcagtt aacggtaaat ccatcttctt tatggtggcg tccaaccctg
    22021 ggcctggctt aggactgttg ctggcgttta ctttgtttgg taaagggatg agtaaacgtt
    22081 ctgcgcccgg ggcgatgatt attcacttcc tcggtgggat ccacgaactg tatttcccgt
    22141 atgtgctgat gaagccgctg accattattg ccatgattgc gggcggtatg tctggcacct
    22201 ggatgtttaa cttactggac ggtggtctgg tggctggccc aagtccgggg tctatctttg
    22261 cttacctggc actgacgccg aaaggctcgt tcctggcgac aattgccggt gttacggtag
    22321 gtaccctggt gtcctttgct atcacttccg taatactgaa gatggaaaaa acggtggaaa
    22381 cggagagcga agatgagttt gctcagtcag ccaatgcggt taaggcgatg aaacaagaag
    22441 gtgcattctc gttaagcagg gttaagcgta tcgcctttgt ttgcgatgcg gggatgggct
    22501 ccagcgcgat gggcgcgacc accttccgta aacgcctgga aaaggcgggg ctggcaatag
    22561 aagtaaaaca ttacgcgata gaaaacgtgc ctgcggatgc ggatatcgtt gttactcatg
    22621 ccagtctgga agggcgcgtg aaacgtgtga cggataaacc gctgatactg attaataact
    22681 atattggcga tccaaaactc gacactttat ttaatcaatt aaccgccgaa cataaaaact
    22741 gattgccgag gtaaaaatga aaaccaaagt tgctgctatt tatggcaagc gggatgtccg
    22801 tctgcgcgaa tttgaactgc cagaaattac cgataatgaa ctgctggtga gtgtgatttc
    22861 tgacagcgtg tgtttatcca cctggaaagc ggcgctactc ggtagtgaac ataaacgcgt
    22921 acccgacgat ttagaaaatc atccggtcat taccgggcat gaatgcgccg gggttattgt
    22981 cgaagtgggt aaaaatctca ccgacaaata taaaaaaggc cagcgttttg tattgcaacc
    23041 ggcaatgggg ttaccaagcg gatattcagc aggctacagc tacgaatatt ttggcggtaa
    23101 cgccacctat atgattattc ctgaaatagc cattaacctg ggctgcgtat taccgtatca
    23161 cggctcttat tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggcgctta
    23221 tcatgccaat tatcacacca cgcaatatgt ttatgagcat cgcatgggcg ttaaacctgg
    23281 cggcaatatt gcgctgctgg catgtgctgg tccgatgggc attggcgcta tcgattatgc
    23341 tattaacggc ggcatacaac cgtcgcgggt ggtggtggtc gatatcgacg acaaacgcct
    23401 ggcgcaggta caaaagctgc tgccggtgga actggcggcc agtaaaggca ttgagctggt
    23461 gtatatgaat accaaaggga tgagcgatcc ggtccagacg ctgcgggcgc tgacggatga
    23521 tgccggattt gatgacgtat ttgtctatgc agcggtgcct tcagtcgtcg agatggctga
    23581 tgaattactg gcggaagatg gctgcctgaa cttctttgcc gggccgacgg ataaaaactt
    23641 caaagtgccg tttaatttct acaacgtcca ttacaacagc acgcatgtcg tcggtacgtc
    23701 cggcggttca acggacgaca tgaaagaggc gattgcgctt agcgccacag ggcagttgca
    23761 gccgtcgttt atggtgaccc atatcggtgg gctggatgcg gtgcctgata ccgtgctcaa
    23821 tctgccggat atccccggcg gtaaaaaact catttataac ggcgtcacca tgccgctcac
    23881 tgccattgcc gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agctggcgcg
    23941 gctggttgag gaaacgcacg gcatctggaa tgagcaggcc gagaaatatc tgctggcgca
    24001 atttggcgtt gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc
    24061 gttacggaac aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa
    24121 attgatggcc tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtggg
    24181 cgagtggtta tcggcgaggg ggaacgatat cctctttagc gctacgggcg tgacgggcgg
    24241 cgagttggtg aacggtatcc aacagacggc gaatggggtg cggacgcaga cattactgat
    24301 cggcggcgcg gaccaaacgt gtaatataat agactccctg cattgatggt gatctatcgg
    24361 aagaccaatg gcgacgctga cagaagatga tgtgcttgag caactggatg cacaggacaa
    24421 tttattttca tttatgaaaa ctgcgcattc tattttgctc caggggatac gccagtttct
    24481 gccgtcgctg tttgtcgata acgatgaaga gatcgtcgaa tatgcagtga agccgttact
    24541 cgcccaaagc ggcccgcttg acgatattga tgttgcgctg cgtttgattt atgcgctggg
    24601 gaaaatggat aaatggctgt acgccgatat cacgcatttt tcccagtttt ggcattacct
    24661 gaacgaacag gatgaaacgc ccggatttgc cgatgacatg acctgggatt ttatcagcaa
    24721 cgtcaatagc ataacctgca atgcgacgct ctatgacgcg ttgaaagcga tgaagttcgc
    24781 cgatttcgcc gtctggtcag aggcgcgttt tagcaggatg gtcaaaacgg cgctgacgct
    24841 ggcagtaacg accaccttaa aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg
    24901 ctgaagatac aggcacagta ccagaacgaa gagattgaaa atgtacataa accgttgctg
    24961 cacatgttgg cggccttaca gacggtaaat ccgcagcggc gtacggtggt ttttctttgc
    25021 gctccgccgg ggacaggtaa atctacccta accaccttct gggaatatct cgcacaacaa
    25081 gacccggaac tgcccgctat tcagacactg ccgatggatg gttttcacca ttacaatagc
    25141 tggctggatg cgcatcaatt gcgccccttc aaaggcgcac cagagacatt caacgttgcg
    25201 aaactggcgg aaaatctgtg ccgggttgtg gaaggggatt gtacgtggcc gcagtacgat
    25261 cgacaaaagc atgatcctgt tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc
    25321 gaaggaaact ggctattact ggatgatgaa aagtggcagg agttggcatc tttctgcgat
    25381 ttttctattt ttattcatgc acctgctcaa attctgcggg agcgcttgat tagtcgtaaa
    25441 attgcgggag gattatcgcg gcaagaagcc gaagcttttt atgcccgaac cgatggtccc
    25501 aatgtcgagc gtgtgctggt gaatagtcgt caggccaatc tgatattaga gatgaccgaa
    25561 gaaggcagat atcacttcaa aagctgacaa tttattccag ttattgcagg ggtattatta
    25621 cgccagcctg gaataaagcc ccctacaaaa ggggataaaa gtgtgatgta agtcagataa
    25681 atgtcttctt cggctggaca atcattcctt ttattccacg tttcgcttat cctagctgaa
    25741 gcgtttcagt cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa
    25801 ggtttccccg gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt
    25861 gcaggagatc tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg
    25921 tggttcgtgc tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg
    25981 aactggcgga tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt
    26041 ttgcatggga agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgtg
    26101 tattgcatga acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc
    26161 tcgactgcac cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg
    26221 ccaaaaaagt gctcttttca catccaggca gtaacgatct cgacgcgacc gttgtttacg
    26281 gcgtcaatca ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca
    26341 cgaattgcat aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca
    26401 ctgtgaccac aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg
    26461 acctgcgtcg cacccgggct gccagccagt cgatcattcc ggtcgatact aaactggccg
    26521 ccggtatcac acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg
    26581 tgccaaccat aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag
    26641 ccaatgaagt caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg
    26701 actatacgga attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg
    26761 tcgatggcac ccaaacccgg gttagtggcg cacacctgat caaaacgttg gtctggtgcg
    26821 ataacgaatg gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg
    26881 ctttcaggta agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc
    26941 atgtctgtaa ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg
    27001 gatctgaacg taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct
    27061 ctgccgacca tcgaacttgc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg
    27121 ggtcgtccta ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac
    27181 ctgaaagaca aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac
    27241 gttgctgaag gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa
    27301 gacgacgaaa ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca
    27361 ttcggtactg ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt
    27421 gcgtgcgcag gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa
    27481 cctgctcgcc cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt
    27541 ctggactccc tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc
    27601 tttatcgcgg cacaaggcca cgatgtgggt aaatccctgt acgaagctga tctggttgac
    27661 gaagcaaaac gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgtgtagca
    27721 actgagttct ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac
    27781 gagcagatcc tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat
    27841 gcgaaaacca ttttgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa
    27901 ggtactgaaa tcgtggctaa tgctatcgca gacagcgaag cgttctccat cgctggcggc
    27961 ggcgacactc tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc
    28021 actggcggcg gtgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg
    28081 ctcgaagagc gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgttgcag
    28141 cgcgctttta aagcacgcac tttttcatac tctaaataat tcgagttgca ggaaggcgac
    28201 aagagagtga atcccatgag cttacataag taagtgactg gggtgagcga acgcagacgc
    28261 agcacatgca acttgaagta tgacgagtat aaggcccgac gatacaggac aagagacatg
    28321 tctaagattt ttgatttcgt aaaacctggc gtaatcactg gtgatgacgt acagaaagtt
    28381 ttccaggtag caaaagaaaa caacttcgca ctgccagcag taaactgcgt cggtactgac
    28441 tccatcaacg ccgtgctgga aaccgctgct aaagttaaag cgccggttat cgttcagttc
    28501 tccaacggtg gtgcttcctt tatcgctggt aaaggcgtga aatctgacgt tccgcagggt
    28561 gctgctatcc tgggcgcgat ctctggtgcg catcacgttc accagatggc tgaacattat
    28621 ggtgttccgg ttatcctgca caccgaccac tgcgcgaaga aactgctgcc gtggatcgac
    28681 ggtctgctgg acgcgggtga aaaacacttc gcagctaccg gcaagccgct gttctcttct
    28741 cacatgatcg acctgtctga agaatctctg caagagaaca tcgaaatctg ctctaaatac
    28801 ctggagcgca tgtccaaaat cggcatgact ctggaaatcg aactgggttg caccggtggt
    28861 gaagaagacg gcgtggacaa cagccacatg gacgcttctg cactgtacac ccagccggaa
    28921 gacgttgatt atgcgtatac cgagctgagc aaaatcagcc cgcgtttcac catcgcagcg
    28981 tccttcggta acgtacacgg tgtttacaag ccgggtaacg tggttctgac tccgaccatc
    29041 ctgcgcgact ctcaggaata tgtttctaag aaacacaacc tgccgcacaa cagcctgaac
    29101 ttcgtattcc acggtggttc cggttctact gctcaggaaa tcaaagactc cgtaagctac
    29161 ggcgtagtga aaatgaacat cgataccgat acccaatggg caacctggga aggcgttctg
    29221 aactactaca aagcgaacga agcttatttg cagggtcagc tgggtaaccc gaaaggcgaa
    29281 gatcagccga acaagaaata ctacgatccg cgcgtatggc tgcgtgccgg tcagacttcg
    29341 atgatcgctc gtctggaaaa agcattccag gaactgaacg cgatcgacgt tctgtaagtc
    29401 attcctttct gcttatctca aggcccgctc tgcgggtctt tttttcgcca aaagcaaggt
    29461 gatgcagatg agaaatctgt gatctatttg gcaaaattat gctttattgt ttacccttgt
    29521 cagactgccc gccataaggc ggcggagtgt atttctccat tttgagtcag ttgaaaagga
    29581 atattgaatg gaagatttga atgttgtcga tagcataaac ggcgcgggaa gctggctggt
    29641 ggctaaccag gcgctgctgc taagttatgc agtaaacatc gtggcggcac tcgcgatcat
    29701 cattgttggt ttgattatcg cgcggatgat ttccaacgcg gtgaatcgcc tgatgatctc
    29761 ccgtaaaatc gatgccactg ttgctgattt tctctctgca ttagtccgtt acggtattat
    29821 cgcctttacg ctaatcgctg cactggggcg cgtgggcgta caaacagcgt cagtcattgc
    29881 tgtactcggt gccgcaggct tagcggttgg tctggctttg caggggtcac tgtctaacct
    29941 ggccgctggc gtgttacttg ttatgttccg cccgttccgt gccggagaat atgttgatct
    30001 cggcggcgta gccggtactg tgctgagtgt gcagattttc tccaccacca tgcgtactgc
    30061 agacggtaaa attatcgtta ttccgaacgg taaaattatt gccggaaata ttattaactt
    30121 ctctcgcgag ccagttcgcc gtaacgaatt tattattggc gtggcgtatg attccgatat
    30181 cgatcaggtt aagcagatcc tgaccgatat tatccagtct gaagatcgca ttttgaaaga
    30241 tcgcgaaatg actgtgcgcc tgaacgaact tggtgcatcg tcgattaatt tcgtggtccg
    30301 cgtctggagc aacagcggcg atctgcaaaa cgtgtactgg gatgtgctgg agcgtattaa
    30361 acgtgaattt gatgccgccg gtatcagctt cccgtacccg caaatggatg tgaactttaa
    30421 gcgggtgaaa gaagacaaag ctgcgtaatc aacgctgagg ccagataata ctctctggcc
    30481 tctctcttat tagtttttct gattgccaat taatattatc aatttccgct aataacaatc
    30541 ccgcgatata gtctctgcat cagatactta attcggaata tccaacgtgt tttcttatta
    30601 ctttcaaggt cttgcacttg gggcggctat gatcctaccg ctcggtccac aaaatgcttt
    30661 tgtgatgaat cagggcattc gtcgtcagta ccacattatg attgccttac tttgcgctat
    30721 cagcgatttg gtcctgattt gcgccgggat ttttggtggc agcgcgttat tgatgcagtc
    30781 gccgtggttg ctggcgctgg tcacctgggg cggcgtagcc ttcttgctgt ggtatggttt
    30841 tggcgctttt aaaacagcga tgagcagtaa tattgagtta gccagtgccg aagtcctgaa
    30901 gcaaggcaga tggaaaatta tcgccaccat gctggcagtg acctggctga atccgcatgt
    30961 ttacctggat acttttgttg tactgggcag tcttggcggg caacttgatg tggaaccaaa
    31021 acgttggttt gcgctcggga caattagcgc ctctttcctg tggttctttg gtctggctat
    31081 tctcgcagcc tggctggcac cgcgcctgcg cacggcaaaa tcacagcgca ttatcaatct
    31141 ggtagtggga tgtgttatgt ggtttattgc cttgcagctg gcgagagacg gtattgttca
    31201 tgcacaagcc ttgctcagtt aggcgctatc tgatggaaaa ataaaacaga ggcgctaagc
    31261 ttgcctccag aggtcctgaa ttttaggcaa gtcaattaac acggagagac taacgtgaag
    31321 ttcaaagtta tcgccctggc ggcattaatg ggtattagcg ggatggcagc gcaggctaac
    31381 gaattgccgg atggaccgca tattgtcacc tccggtacgg caagcgtgga tgcggtgcca
    31441 gacattgcca ctcttgcgat tgaagtcaac gtggccgcga aggatgccgc tactgccaag
    31501 aaacaggcgg atgagcgcgt cgcacaatac atttccttcc ttgaactcaa tcagatcgcg
    31561 aaaaaagata tcagctcagc gaacttacgc acccagccag attatgatta tcaggatggt
    31621 aaaagtatcc tgaaaggcta ccgtgctgtg agaacggtgg aagtcacgct ccgtcagtta
    31681 gacaaactga attccttgct ggatggcgcg ctgaaggcgg gtcttaacga aattcgttct
    31741 gtatcgctgg gcgtggcgca gccggatgcc tataaagaca aagcgcgtaa ggcagcgatt
    31801 gataacgcga ttcatcaggc gcaggaactg gcgaacggct ttcatcgtaa actggggccg
    31861 gtatatagtg tgcgctacca tgtttccaac tatcagccca gcccaatggt gcggatgatg
    31921 aaagccgatg ccgcgccggt gtccgcccag gaaacttacg agcaggccgc tattcagttt
    31981 gatgatcagg tcgatgtggt cttccagtta gaacctgtgg atcaacaacc cgctaaaaca
    32041 cctgcagcac aataatattt gtagcccgac gtattcgtcg ggctaatgcc tttacccgat
    32101 attgcctcct ttttctgtaa ttttctgtaa aaccatgacg taatcaccta tctttgctcg
    32161 cggtttcatc aaggataggc tgactgaaac ggttaagcgg acaaggatgc gggggagggg
    32221 gtatggacat ttttatttcg aaaaagatgc gcaatttcat tttattagcg caaaccaata
    32281 atattgcccg ggcggcagaa aaaatccata tgacagcttc gccatttggt aaaagcattg
    32341 ccgcgctgga agaacaaatt ggctatacgc tatttacccg caaagataat aatatcagcc
    32401 tcaataaagc cggacaggaa ctgtatcaaa aactgttccc ggtttatcaa cggctttctg
    32461 cgattgataa tgaaatccat aactcagggc gtcgttcacg agaaattgtg attggcatag
    32521 ataatactta tccgacaatt attttcgatc agttgattag tctcggagat aagtatgaag
    32581 gcgtgaccgc ccagccagtt gagttcagcg aaaatggcgt tatcgataat ctttttgacc
    32641 gtcagttaga ttttattatc tccccacagc atgtatctgc acgagttcag gaactggaaa
    32701 acctgactat cagtgaacta ccgccgctgc gattgggctt tctcgtttct cgtcgttatg
    32761 aagagcgaca agagcaagag ttgcttcagg agctaccctg gctacaaatg cgctttcaga
    32821 accgggctaa cttcgaagca atgattgatg cgaatatgcg tccttgtggg atcaatccca
    32881 ccattattta tcgtccctac agttttatgg ccaaaatcag cgccgtggag cgcggacatt
    32941 ttctgacggt gatcccgcat tttgcctggc gactggtgaa tccggcaacg ctgaaatatt
    33001 ttgatgcgcc gcacaggccg atgtatatgc aggaatatct ttattcaatc agaaatcatc
    33061 gttataccgc cacgatgctt cagcatattg ctgaagatcg tgacgggacg agtcattaac
    33121 ccagcatcga gccggttgca attaaattac ggtgtaagtc gaagacgtgg ctaagatcgt
    33181 ggtgaatatg tccgccgggc gcattttcca gatagcgatg cagataatcc cgatacatag
    33241 gatgtgcaca attatcaatg atagtgcggg cgcgttgaag cggggaaaga ccacgcagat
    33301 cggcgatgcc ttgttcggtg atgatcactt tgacgctgtg ttcgctgtga tcaacatggc
    33361 tgcacattgg cacgacggtt gagatcttgc cttctttagc aatcgacggg gccataaaga
    33421 tcgacagata cgcgttgcgt tcaaaatcac cgctgccacc gatgccgttc atcagatcga
    33481 ccccggctac gtgcgttgag ttggcatgcc cgtaaatatc aaactccagg ccgacgttca
    33541 gagcgatgac gcccagacga cggatgattt ccgggttatt ggaaatctcc tgcggacgca
    33601 acacaatgcg gctggcaaag taatccatat tgtcgtaaat cttgcgcagg gaatcggccg
    33661 agatagtcag gctggaggcg ctggccccgc tgattttgcc cgtttccagt aaatgcacca
    33721 ccgattcctg tagcacttcc gaatacatca taaacggagg aatttccgga ttttccccca
    33781 gacgcgccat taccgcatta ttgatgttgc ctacgccact ttgcagcggc agaaattccg
    33841 gcggaatacg cccatgcgcc atttcctgca acaagaacgt gaccacgtta tcggcaatct
    33901 gctggcacat gggattttgc ttatccagca tattaccggc gtcaggcaag ttggtttcca
    33961 cgacggcgac aatctttttc ggatcgattt gcacatagcg ggtaccgacg cgatccattg
    34021 catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc aggaatcaca atatctgcca
    34081 gttctgcaac gcgcggatcg tgatagtgat tgagttcaat gatcactttc ttcgcccgca
    34141 gcagccaggt cggcgcatta ccgatcccgc tggttaacca gactcgacca tccggtgcca
    34201 gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa gaaaccgtaa ttgaccattt
    34261 gcgccacttc gctcaaatgc aggtcaacga aactcaccgc gccctgattg atctttttac
    34321 gtaaaccgga cgatgtttga tatggcgcac gccaggaaac agcatcggcg tcagaaagta
    34381 catcgtcagc ggcggcgctg attgacgccc ccgtcagcag gcgaatttga tacggctttt
    34441 tggcctcatg ctgttcgtta gctctgcggg caatcgcgtt gggtaaggct ttcggcgaac
    34501 ccgctggggt aaagccgcta aatgccacca tgtcgttatg ctggataatt tctgccgctt
    34561 catcggcggt catccttgtc cactgagttt ccattttaac tccttcgttc gcagggatta
    34621 atgaccaacg aaattaggtt tacgtttttc gaggaaagcg ttcatccctt cctggtaatc
    34681 ttcgctgtca taaaccgcgc ggcgcatccc ctgaatacgt tcaaattcat cggagttcat
    34741 ggtgtgcgct tcgcccagta cacgcagctc ttctttgata acggcaatgg ctaacggcgc
    34801 tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct tccagttctt ccacttccac
    34861 aacatggttg aggatgccga cagccagcgc gcgctgggct gtgattggcg aagcggtaaa
    34921 aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc aggttgtgaa tgccgaccag
    34981 gttatacggg acgccgaggt ttacaggtgt cattgagaag gttgaggtac tggcggcgat
    35041 gatcagatcg gaactcatga tcatttcaaa tgcgccaccc cagacactac cttccaccat
    35101 cgaaatgatc ggtttcggga atttttggat catgcgggtg atttgacgca acggatcatc
    35161 ataggagagc ggatcgcgac cgccagacgg cagttcgtga atatcgtgac ctgcggagaa
    35221 gactttggaa ccactcggcg cgcgcaaaat gatacagcga atttccggcc ggttgagatc
    35281 gcttaatgcc tgcataagat catcaataaa gactttactt aaggcattaa gtttgcggcc
    35341 atagttaaac tcaatgaccg ccactttgtt gatagtgaca acgttcacat actgataaga
    35401 cataaaaatt cctttaatca aaatattgcg tctggataaa ttcactgagc tgccgcaggc
    35461 cggtgcgcgg tgagagcgta ttgtttttga ctgctaaaag cgtctggcgg taatagcgat
    35521 cgaaatcttc attcgcgaac aggcgattca gtacttcttc ttcggtctgc ttacgcagcc
    35581 attccaccga ttgttgttgc cgcacttgtt gtaatcgacc actggcagtt agcgcggttt
    35641 tgaagtcgat gatggcgtgc cagatctcat cgattccacg tttttccagt gcgctacaag
    35701 tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag aatatgcagg gcactctcgt
    35761 acatatgccg ggcaatggcg acattggtat ggttatcgcc atcgtctttg ttgataacga
    35821 tcagatctgc cacttccatc agcccctttt taatgccctg cagatcatcg ccgccaccgg
    35881 caatttgcaa cgagataaaa cagtccacca tgcgggcgac ttccgtttct gactgcccga
    35941 cgccaaccgt ttcgacaatc actacgtcat aacccgccgc ttcgcataac agcattaatt
    36001 cccgcgctcg ctgactggca ccgcccagat ggccggagga gggtaccggg cgaataaacg
    36061 ccgcctcggc acgcgcaagg tcattcatgc gggttttatc cccgagaatg ctaccgccag
    36121 tgaccgggct gctgggatcg accgcaataa ccgcaacctt taatccctca cgaatcaaca
    36181 acatgccaaa ggcttcaaga aaggtacttt tccccgcgcc gggtgtgccg gtaacgccca
    36241 gtcgcagggc gttaccacag aacggcataa tggcgtcaag cagctgtgta cttagcgcct
    36301 gatgacgcgg gtgacggctt tccaccaacg tcatggcctg ggcgagcgtg gcacgctcac
    36361 cctgacgtaa gcggcgaata ctttctgcta gcttggcttc attaatcatg atgctggctt
    36421 atcagattca gtacgtcgcg cacgctgtcg agcataggtg tacctggacc ataaatcgcc
    36481 gccacgccgc gctcttgcag gaaggcgtaa tcctgcggcg gaatgacgcc acccgcgacc
    36541 acgcagatat ctttgcgtcc ccattttttc agcgcttcga ccagttccgg gatcagcgtt
    36601 ttatgaccgg cagccagtga ggatgcgccc actacgtgaa cgtcgttttc tacggccagg
    36661 cgggcgatct cttcaggtgt agagaacatc gggcttaaat ctacgtcgaa accgagatcg
    36721 gaataggcgc tggcgatcac tttcgcgccg cgatcgtgcc catcctggcc cattttggcg
    36781 atcagaatgc gcgggcgacg accattgtcg gcaaggaact gctccgtttg cgcaacaatg
    36841 gcatcgaact cggcggccga tttctcagac tgatgatagc tttgcgcaat cacgccggta
    36901 acacactggc ttggcaccag ataacggtcg aaagcggctt ccagcgcatc ggaaatttca
    36961 cccagggtgg cgcgaacgcg agcagcatta acagcggcag ccagcaggtt ttcgttatgc
    37021 tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg cggcatcatc acgggtggcg
    37081 cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca tcacgttgtc gatctcaagt
    37141 acatcggttt cgtcttcgtg atccagtttg tacttgttga caccaacgat gacacgcttg
    37201 ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt cgatcattcg ttttggcaga
    37261 cctgcttcga tcgctttcgc catgccaccg gcttcgtcga tctgttggat aatagctctg
    37321 gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat gggatccggc cagtggatcg
    37381 acggtgcggc agagttctga ttcttcctga atgatgatct gggtattgcg ggcaatgcgt
    37441 gctgagaaat cggtaggcaa accaagcgct tcatcaaagg cgttggtatg cagtgactga
    37501 gtaccgccca gcgttgcagc cagtgcttca atggtggtgc ggataacgtt gttatacgga
    37561 tcctgttcag tcaggctcca gcctgaggtc tggcagtggg tacgcagcgc cagtgatttc
    37621 ggatcctgtg cgccaaatcc actgaccgct tcgctccata aataacgtgc cgcacgcaac
    37681 atggcgacgt tcataaacag atccatgcca atgccgaaga agaacgacag acgaggagcg
    37741 aagtcatcaa ttttcagccc ggcggagatt gctgctttga tgtactcaat cccatcggcg
    37801 agcgtaaatg ctacctgctg cacgcagttg gcacccgctt cacccatgtg gtaaccgctg
    37861 atactgatgg tattaaatcg cggcatgttg ccggaacacc aggcgatgat gtctgcgata
    37921 atacgcattg acggttttgg cgggtaaata taggtgttgc ggcaaagata ctctttgagg
    37981 atatcgtttt gaatggtgcc ggtcagttta tcgggtgtaa caccttgctc ttctgcggcg
    38041 acaatataaa acgccagtac tggtagtact gcgccattca tggtcatcga aaccgacatt
    38101 ttatccagcg ggatctggtc gaacaggact ttcatatctt ccacggtgtc gatagcaacg
    38161 cccgctttgc cgacgtcgcc cgccacgcgt gggttatcgg agtcgtagcc tcggtgggtg
    38221 gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg ccaggttacg gcgataaaaa
    38281 gcgttggact cttttgctgt tgaaaaacca gcatactgac ggatggtcca cggttgggcg
    38341 gtatacatag tggcacgcgg gccacgaacg tagggaggca aaccaggaag ggtacctgtc
    38401 acctccagat tatcgagatc ggcttcggta tacagcggct tgatggcgat cccttccgcg
    38461 gtttgctgaa ccagcgagtc gacagttttc tcccgacggc tcaattcctt gttggcaagc
    38521 tgttgccact cctgcacctt agacattatt tgctccgtaa aacgcaatca aaatgagggt
    38581 atttgcgctt acggtgagct ttttacgtgg gtttgtcgcc agcaaaaaaa atgcattcag
    38641 acgtcgtttt ttgccgcctg agatttgtct attttgtgat gtgattcaag gaatacaaaa
    38701 agaagtgagg ataacgcctg atatgtgcta tcaggcatta tttgatggat taatcctgac
    38761 gaaggacttt gtgaccataa tcgagcaacg catcagtgac tttacgcatc atgcggcttt
    38821 caggagcaaa gcggtgccag tagagcatcc gtcgttgaaa tagccctggc gttaagtcaa
    38881 tcagttcacc gctggccagc tctttctcga tttgcaggtg cgggatcata cagcaggtgg
    38941 tgccctgacg agcaagttgc acgaacgctt ccgaagaatt aacgatatgg cagggtacgc
    39001 tgccaggagg cagatcgaag ttttgctgca aaaaggcctg gtgcatatcg tcaagatggt
    39061 caaaagcgac cacaggtgct ttcaataatg ccgaacgcgt tacgccgtta gggaaatatt
    39121 tttcggcaaa cggttttgag ctgacgaaca gatagtcgag cgcaccgagc ttatcgacaa
    39181 ggcaactcgg cagcgcctga tgttgaatac tcaccgcgcc gaccacttcg ccgcggcgca
    39241 gacgttcctg agtgcgggtt tcatcttcta cctgcaagtt gaggcggata ggcgaatcag
    39301 ctaacacagg agccagcgcc ggaagcaacc acgtcgccag actgtcagcg ttgaccgcca
    39361 gtgaaagcag cagcggagtt gaaccggttt gttcatcgcc cagccactct tcttccagta
    39421 actccacctg gcgtagcagt gccagcagtt tttgcccttg ttctgtcggg cgcggtggta
    39481 cggtacgcac caacagcggc tgcccgaaca tattttccag ttgcttaatg cgctgtgaga
    39541 cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc aaatcctcgt tcacgtatca
    39601 ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt cattgctgtt gtctgctcct
    39661 ggtaaatgct ttttcctgca ctatgacaca attttgtgtc aggttgcata taccgaagtc
    39721 gttggcgggt aacatttgta tcagtggtta acgcgcagct taaaagatca gggatttgcg
    39781 ctaatgcatt tcgctcagcg agttcgcgcc ctggttgtgt tgaatggcgt ggcgttattg
    39841 cctctgttcg catcgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga
    39901 aaggggatgc ccgccattgc gcgatatttt atggatgagt taaccacccg gcttgccaac
    39961 ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac
    40021 gatcatgacg caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca
    40081 gcccggcacc attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcgct
    40141 cggcacgatg aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa
    40201 actgaaaagc ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta
    40261 cgttgatggc gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc
    40321 gctgacccgt gaaaaaatca ttgcttcggt tgcggaaaaa tttatctgta ttgccgacgc
    40381 ctccaagcag gttgatattc tgggcaaatt cccgctgcca gtagaagtta tcccgatggc
    40441 acgtagtgca gtggcgcgcc agctggtgaa actgggcggt cgtccggaat accgtcaggg
    40501 cgtggtgacc gataacggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc
    40561 gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gcttgtttgc
    40621 taaccgtggc gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa
    40681 atgatctgac gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat
    40741 gtgtcacgtt tttacctggc tattgtcgat tgctctaaat aaaccctcta aaccagcata
    40801 ttcatccaag aaataccttt gcgtgatatt tcctcaatat cgtgacgcaa acgttcatat
    40861 tgccgcaata ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc
    40921 aaaagacagg attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct
    40981 gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagcgg gttacaccaa
    41041 catcgaattt cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc
    41101 ccacttcatc ggcctgcgat cccgtaccca tctggctgaa gacgtgatca acgccgcaga
    41161 aaaactggtc gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc
    41221 ggcaaaacgc gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga
    41281 gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc
    41341 gcatcgtggc gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct
    41401 gggtatcatc ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg
    41461 aatgtatgtt tacttttatg atattgaaaa caaactgccg ctgggcaacg ccactcaggt
    41521 acagcatctt tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa
    41581 tccgtccacc aaaaatatga tgggcgcgaa agagatttcg ctaatgaaac ccggctcgct
    41641 gctgattaat gcttcgcgcg gtactgtggt ggatattcca gcgctgtgtg acgcgctggc
    41701 gagcaaacat ctggcagggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag
    41761 cgatccattt acctctccgc tgtgtgaatt cgacaatgtc cttctgacgc cacacattgg
    41821 tggttcgact caggaagcgc aggagaatat cggtctggaa gttgcgggta aattgatcaa
    41881 gtattctgat aatggttcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact
    41941 gcacggtggt cgtcgtctga tgcacatcca cgaaaaccgt ccgggtgtgc tgactgcgct
    42001 caacaaaatt tttgccgagc agggcgtcaa catcgccgcg caatatctgc aaacttccgc
    42061 ccagatgggt tatgtagtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct
    42121 gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aaatcccctt
    42181 atctgaaaat caacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg
    42241 ttgccgcatt gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc
    42301 aatgcgttgt gtttatttaa tggggattta cgatggcagg gcagcatggg gctgtaacgg
    42361 taaagccctc accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat
    42421 actgattgta ctgttactca taagtttccc agcttattaa cagtcagtct caggggagga
    42481 gtcatcctcc cttaccctta ctcactaaat taggttaaag aatcaacgat gtcaatcagg
    42541 gcgatgcagg tgtgtcgccc tcaccactcc cagactttcg acggtgtaac caccgcagga
    42601 agagggatat cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa
    42661 cccaccggtt gcattttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg
    42721 cccattccca ggcgctgacc gtactcatca aaggcgacca gcggtgtgat caacacgtct
    42781 aatcgggaaa ggggtagcac gtcacgcaca tccagtttgg gctcatggat cttcaacctg
    42841 ttcatcacca gttcgctttg cggatggtaa ttcaggaaca gcaaattacc ggcactgaag
    42901 ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc agagttgttc tatgagtggc
    42961 agggtgtcga gttcgccatc aaaagagagg aatacagcga ccgtatgtgc catcaccacc
    43021 gggggataag tcatcatccg ggtagcggct tgttgaccca tttcctgctg ttgttccggc
    43081 gttaacgcac gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt
    43141 gggagttctg gtagttgcgt catggctggt gccagataag aagggaatct ccgagatgcc
    43201 gccgcaggct gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag
    43261 gcttctcgga cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga
    43321 aatatcggct caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg
    43381 ttactctacc acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga
    43441 tgcgaccttg ttcaaataac gcttgttcta tggtctgctg cagcatccga atacgctgtt
    43501 ccatacttgc cgcatagtca cgagtcttcg ctttttcttg cgctaactca tagctgatat
    43561 tcaatgcggc aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat
    43621 cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg
    43681 ggcagttcac acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc
    43741 caccttcctg ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc
    43801 gacactatag ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa
    43861 gtggtagcat atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac
    43921 gaaatgcctg gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc
    43981 cctgctgaga tgcatggttt aatcagcggg atgatatgtg gcggtaacga tgacagctca
    44041 tggctgccgc tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca
    44101 caggcactgc gtaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt
    44161 tttcagcttt atctgcctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcgctg
    44221 gcaggttggg tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa gctggacaaa
    44281 gtgaccggag aaaccggtga agccatcgac gatctgcgta acatcgcgca gctgggctac
    44341 gacgaagacg aagatcagga agagcttgaa atgtcgcttg aagagatcat cgaatacgtt
    44401 cgtgttgccg cgctgttatg ccacgacacc tttactcatc cgcaaccgac cgcgccagaa
    44461 gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgagat
    44521 atcccggcaa gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc aacccggcag
    44581 cgccgcgctg atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta
    44641 tcgtcagaac agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgctggt
    44701 gctgattaaa agcgacgaca ctcataacca cagcgttttg tttaaccgcg ttcgcgacct
    44761 gacggcagag atctggtttg gtcgtcgctt aggccaggat gccgcgccag agaaactggg
    44821 cgttgaccgc gcactggcat tcagcgaaat caatcagcaa ctttatcaac tgcttaatgg
    44881 tctggatgtg gtttatcacg ctcagggcga atatacctat gctgatgaaa tcgtgaacag
    44941 cgcgctggaa aagctgcgta aaggctcgcg acagaatctc actgccccgg ctacgatgat
    45001 cgactggcgt cctgttgttc atgaaatgcg cctgttcaaa tcaccagaag agattgccgt
    45061 acttcgccgc gcgggtgaaa tcaccgcact ggcgcatacc cgggcgatgg aaaaatgccg
    45121 tccggggatg ttcgagtacc atctggaagg cgaaattcac cacgagttta accgccacgg
    45181 tgcgcgttac ccttcctata acaccattgt tggcagcggt gaaaacggct gcattttgca
    45241 ctacaccgaa aacgagtgtg aactgcgtga cggtgatctg gtgttgattg acgcgggttg
    45301 tgaatacaaa ggttacgctg gcgacattac ccgcaccttc ccggtcaacg gcaaattcac
    45361 tcaggctcag cgtgaaatct atgacattgt gctggagtct cttgaaacca gcctgcgcct
    45421 gtatcgtccg ggaacttcca tgcaggaagt cactggtgaa gtggtgcgta tcatggttag
    45481 cggcctggta aaactcggca tcctgaaggg tgatgttgat gaactgatcg ctcagaacgc
    45541 ccatcgtcct ttctttatgc atggccttag ccactggtta ggactggatg tccatgacgt
    45601 tggcgtttat ggtcaggatc gctcgcgcat tctggaaccg gggatggtac tgaccgtaga
    45661 gcctggtctg tatatcgcgc cggatgcgga tgtgccagaa caatatcgcg gtatcggcat
    45721 tcgtattgaa gacgacattg tgattaccga aaccggcaac gaaaacctca ccgccagcgt
    45781 ggtgaaaaag ccggaagaaa tcgaagcgtt gatggctgct gcgagaaagc aatgagcgta
    45841 atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt
    45901 cacggggcgc tgccggtaca tttgattgaa gcgactgcgc cagagtcaca tgctcatccg
    45961 ggctttgatg gacgagcgat agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc
    46021 ggcgtctggc aatctctggc ggattgcgca accgccatca ctaccgtgca tgtcagcgat
    46081 cgtggtcatg ccggattcgt caccctcgcc gcagaagatt accaactggc ggcgctgggg
    46141 caggttgtcg aattgcataa tgtcgggcaa cggctgtttg cattgctgcg caaagcacct
    46201 ggcgtaacgc tgcattgtcc tgatcgcgtg gctaacgttg cccgtactca gagtcacgtt
    46261 gaagtgacgc tggagagtgg cgagacgctg acgggctgcg tgctggtagc cgctgatggc
    46321 acccattcag cgttagccac cgtgtgcggc gttgactggc agcaggagcc ttacgaacaa
    46381 ctggctgtga ttgccaacgt tgcgacttcc gttgcgcatg aagggcgcgc ttttgaacgc
    46441 tttacacaat atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc
    46501 tggtgtcatc cactggaacg gcgcgaagag gtactgtctt ggagcgacga gaagttttgc
    46561 cgtgaactcc agtcggcctt tggttggcga cttgggaaaa ttacccacgc tggtaaacgc
    46621 agtgcttatc cgctggcgtt aacccgcgcc gccagaccta ttacccatcg taccgtgctg
    46681 gtgggcaatg cggcgcaaac tctgcacccg attgccgggc aagggttcaa cctcggtatg
    46741 cgagatgtca tgagccttgc ggaaaccctg actcaggcgc aggagcgcgg agaagacatg
    46801 ggtgattacg gcgtattgtg ccgttatcag cagcgtcgac agcgcgatcg cgaagcaacc
    46861 atcggcgtca cggacagcct tgtacatctt tttgccaacc gttgggggcc gctggttgtc
    46921 gggcgcaaca tcgggctgat ggcaatggaa ttattcaccc cagcacgcga tgtgctggcg
    46981 cagcgcaccc tcgggtgggt ggcgcgttga aggcggtttt aaggaataaa caatgcaaag
    47041 tgttgatgta gccattgttg gcggcggcat ggtggggctg gcggttgcct gcggcttgca
    47101 gggtagcggc ttacgcgttg ccgtactgga gcagcgcgta ccggaacctc tggcggcgga
    47161 tgcaccacca caactgcgcg tttcggctat caatgccgcc agcgaaaaat tactcacccg
    47221 tcttggtgtc tggcaggaca ttctctctcg tagggccagt tgttatcacg gtatggaagt
    47281 gtgggacaaa gacagttttg gtcacatttc gtttgacgat caaagcatgg gctatagcca
    47341 tcttgggcat atcgttgaaa actcggtgat tcactacgcg ctgtggaaca aagcgcagca
    47401 gtcgtcagat atcactctgt tggcccccgc agaattacag caggtcgcct ggggagaaaa
    47461 tgaaaccttc ctgacgttga aagatggcag tatgttaacg gcgcgtctgg tgattggcgc
    47521 ggacggcgct aattcctggt tgcgcaacaa agccgatatt ccgctgactt tctgggatta
    47581 tcagcatcac gcgctggtag cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg
    47641 gcaggttttt catggcgaag gcattctggc ctttttgccg cttagcgatc cgcatctttg
    47701 ctcgattgtc tggtcactgt cgccagagga agcgcagcgg atgcagcagg caggcgaaga
    47761 cgaatttaat cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat gcaaggttga
    47821 aagtgagcgc ctggtgttcc cgctaacggg gcgttatgcg cgccagtttg ccgcacaccg
    47881 tctggcgctg gtgggcgacg ccgcgcatac tattcacccg ctggcggggc agggggtaaa
    47941 tctcggcttt atggatgctg cagagctgat tgctgaactg aaacggttgc atcgtcaggg
    48001 taaagacatc gggcagtaca tttacctgcg tcgctatgag cgtagccgta agcacagtgc
    48061 ggcgctgatg ctggctggta tgcagggatt ccgcgatctg ttttccggta ccaatccggc
    48121 gaaaaaattg ctgcgtgata ttggtctgaa actggctgat acgcttcctg gcgttaagcc
    48181 acaacttatt cgtcaggcaa tgggattaaa cgatttgcct gaatggctgc gttaaatatt
    48241 tctcttctgc tgtttatttg atacccatca cactttcatc tcccggtttt ttcgccggga
    48301 gattttcctc atttgaaata aactaattta acctccgttt tcgcattata ttttctaatg
    48361 ttatggattt ttgatttagt gttttttgac atttttttag cgcttaatat tgtcttattc
    48421 aaattaactt tctcatcaca tcatctttgt atagaaactg atgtgttttt tggtttttta
    48481 ttctgttgcg atttttgcat tttttaacca taagctaatg tgatgatcaa ttttacctta
    48541 tggttaacag tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc gttggggacc
    48601 gggagtggct ccgatgctgg gtttcgtggt gataatttcg ccatggaaaa gttgtcagcc
    48661 ccgcttattc aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct
    48721 ttgcggcgct cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca
    48781 aatcgacgaa catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac
    48841 catcgtcgat cttcgcggca gccgcactcg ggagtttctg cgttatctgc tggcgaacga
    48901 tgtggcgaaa ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg
    48961 cggtgtgata gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt
    49021 taactccgcc acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg
    49081 catcgaaatt accgttcgtg atgacctttc catgatcgcc gtacaagggc cgaatgcgca
    49141 ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc
    49201 gttctttggc gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc
    49261 gggctatgaa attgcgctgc ccaatgaaaa agcggcggat ttctggcgcg cgctggtgga
    49321 agcgggcgtt aagccatgcg gcctgggcgc gcgtgacacg ctgcgtctgg aagcgggtat
    49381 gaatctttat agtcaggaga tggacgaaac catctctcct ttagccgcca atatgggctg
    49441 gactatcgcc tgggaaccgg cagatcgtga cttcatcggt cgtgaagccc tggaagtgca
    49501 gcgtgagcat ggcacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg
    49561 taatgaactg ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac
    49621 cagcggtact ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg
    49681 tattggtgaa acagcgattg tacaaattcg caaccgtgaa atgccggtta aagtgacgaa
    49741 acctgttttt gtgcgtaacg gtaaagccgt cgcgtgattt acttttttgg agattgattg
    49801 atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa
    49861 gccggcggca cttacaccgt tggtatcacc gaacatgccc aggagctgtt aggcgatatg
    49921 gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt
    49981 gccgaatcag taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg
    50041 gtaaacgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggcggc
    50101 tggatcttta aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc
    50161 gcatacgaag cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat
    50221 cagggtgagg aacttttacg cctcaccctc actctcttcg caaggagaga ggttcacaat
    50281 tcactgcacg tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag
    50341 cggcgctttt attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa
    50401 tgccgttggc gcacaatcgt taaacgcgct gaccggccag attgtgccga aagatattca
    50461 gcttgcgacc ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcgc tggcggaact
    50521 caaggctatt gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc
    50581 cgtgcagcta ccgccggtta ttctgcgtaa catgctggaa aatccgggct ggtataccgc
    50641 gtatactccg tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca
    50701 gcaggtaacg ctggatttga ccgggctgga tatggcctct gcctctcttc tggacgaggc
    50761 cactgctgcc gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa
    50821 ccgcttcttc gttgcttctg acgttcatcc gcaaacgctc gatgtggtcc gtactcgtgc
    50881 tgaaaccttt ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga
    50941 cgtcttcggc gtgctgttac agcaggttgg cacaaccggt gaaatccacg actacactgc
    51001 gctcatcagc gaactgaaat cacgcaaaat tgtggtcagt gtcgccgccg atataatggc
    51061 gctggtgctg ttaactgcgc cgggtaaaca gggcgcggat attgtctttg gttcggcgca
    51121 acgcttcggt gtgccgatgg gctacggtgg cccacacgca gcattctttg cggcgaaaga
    51181 tgaatacaaa cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa
    51241 taccgctctg cgcatggcga tgcagactcg cgagcaacat attcgccgtg agaaagcgaa
    51301 ctccaacatt tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta
    51361 tcacggcccg gttggcctga aacgtatcgc taaccgcatc caccgtctga ccgatatcct
    51421 ggcggcgggc ctgcaacaaa aaggtctgaa actgcgtcat gcgcactatt tcgacacttt
    51481 gtgtgtggaa gtggcagaca aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa
    51541 cctgcgtagc gatatcctga acgcggttgg gatcaccctt gatgaaacca ccacgcgtga
    51601 aaacgtgatg cagcttttca gcgtactgct gggggataac cacggcctgg acatcgacac
    51661 gctggacaaa gacgtggctc acgacagccg ctctattcag cctgcgatgc tgcgcgacga
    51721 cgaaatcctc acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta
    51781 tatgcactcg ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc
    51841 ctgcaccatg aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc
    51901 cgaactgcac ccgttctgcc cgccggagca ggccgaaggt tatcagcaga tgattgcgca
    51961 gctggctgac tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc
    52021 tggcgcacag ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa
    52081 cgaagggcat cgtgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc
    52141 tgcacatatg gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga
    52201 tctgactgat ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt
    52261 gacctatcct tccacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt
    52321 gcatcagttc ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggtcggcat
    52381 cacctcaccg ggctttattg gcgcggacgt ctcgcacctt aacctgcata aaactttctg
    52441 cattccgcac ggtggcggcg gccctggtat gggaccgatc ggcgtgaaag cgcatctggc
    52501 accgtttgtg ccaggtcata gcgtggtaca aattgaaggc atgttaaccc gtcagggcgc
    52561 ggtttctgcg gcaccgttcg gtagcgcatc cattctgcca atcagctgga tgtacatccg
    52621 catgatgggc gcagaagggc tgaaaaaagc aagccaggtg gcaattctta acgccaacta
    52681 tattgccagc cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt
    52741 ggcgcacgaa tgtattctcg atattcgccc gctgaaagaa gaaaccggca tcagcgaact
    52801 ggatattgcc aagcgtctga tcgactacgg tttccacgcg ccgaccatgt cgttcccggt
    52861 ggcgggtacg ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt
    52921 tatcgacgcg atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc
    52981 gctggaagat aacccgctgg tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg
    53041 ggcgcatccg tacagccgtg aagttgcggt attcccggca ggcgtggcag acaaatactg
    53101 gccgacagtg aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt
    53161 accgattagc gaataccagt aattcactga ttcgactatc ttctaaaggc gcttcggcgc
    53221 ctttttagct tgttaataaa gtgcgctttg tgtgtcggat gaagcgtaaa tataaactcc
    53281 tggagttcaa tatatgacag gagcgccagg cctgatgagc ggggcgcatc aggcacatcg
    53341 ggtaattaaa gcttctcgcc gttgctggca atcacttctt tgtaccagtt aaagctcttc
    53401 ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg
    53461 tagcgtttgc tgtactgccc ggtagtgaac gacacgcaat cgatgcagcc ccacggtgtg
    53521 tagcccatca gatccacgcc atcgtaagtc accgcttttt tcatctcttc aatatgggcg
    53581 cgcaggtagt caatgcggta gtcatcgttg atgctgccat cttcttccac tttgtcataa
    53641 gcgccaaaac cgttttcgac aataaacagc ggcttctgat aacgctcata cagttcgcaa
    53701 agtgcatagc gcagacccac aggatcaatc tgccagcccc agtcagacgc tttaacatac
    53761 gggtttggta cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
    53821 accgcgttgg tcatgtaata gctgaaaccg agataatcgc aggtgccttc gcgcagcaca
    53881 tcgagatcgc cgtcttccat tttgatgtta aatccgcgac gctcccactc gtgcaacaca
    53941 taggacgggt aatagccacg cagttgcaca tcggtaaaga cgtagcgttc gcgcatcgac
    54001 tcctgggcga acatcacatc gtccggatta caggagtaag ggtaaagcgg caccatcgcc
    54061 agcatacagc cgactttcat ttccgggtta atgcgacgcg cggctttcac cgccagggcg
    54121 ctggcgacaa actggtgatg cagcacctgg tacatcgtct cttccgggtt ttcatgctcg
    54181 gtatacacca cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg
    54241 ttaatttcgt tgaaggtcat ccagtatttg actttatgct tatagcgttc aaataccact
    54301 tctgcgaaac ggacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc
    54361 accagatgca gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc
    54421 agcagttcat cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca
    54481 tcgccttttg gaaaaatgcg cgtccaggcg atggatgtac gaaaacattt gaagcccatt
    54541 tcggcaaata gcttgatgtc ttccttgtaa tgaccataaa aatcaacggc ttcatggttc
    54601 ggatagtatt ttcctggcaa cacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
    54661 ccggtcagaa cgtcacaaat gctcggccct ttgccgcctt tgttccagcc gccttcgacc
    54721 tgatgagcgg caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact
    54781 atcataggct ccagatgagt caatttttac aggaaatagt accatgcagc caaacgacat
    54841 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
    54901 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcgggc gttttgaaga
    54961 cgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact
    55021 gacggaaaaa catgcagagc aggaaaatat gaccctgact gatctgaaaa aggtcattgc
    55081 cgatatctat cccggccaga cacagtttta tgtgattgaa tttaaatgtc tttaaattga
    55141 actcacgcct gttagttgaa atttcgcatt taatttcatg atttttcgga gattagtctc
    55201 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
    55261 aggtatacat gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta
    55321 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg
    55381 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
    55441 ccacagcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
    55501 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
    55561 attgcgctat ttacctattg attgccggaa cctacacgcc gtttttgctg gtggggctgg
    55621 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc
    55681 tgttcaaact gaccatcgcg caccgattca aaattttatc tctggtgacc tatctggcga
    55741 tgggctggct gtcgctggtg gtgatttatg aaatggcaat taagcttgcg gcgggcagcg
    55801 ttaccttact ggctgtaggc ggcgtggttt actcgctcgg ggtgattttc tacgtctgca
    55861 aacgcattcc ctacaaccat gccatctggc acggctttgt gctcggcggc agcgtgtgcc
    55921 actttctggc gatctattta tatattgggc aggcgtaatg ggtgtgcctt tgtaggcctg
    55981 aaaagacaca cccttgtcgc atcaggcgat aaatgcatga tgcgacacag agatttactc
    56041 ttcgagtgaa tacggcagcg gctcgatatg caatgtattc gcatcgtcac gcacgcggaa
    56101 gatgctgtcc ggttccatat cgttattcat gacgacctgt accacgacct gaccatcttc
    56161 cagttttacc gcagccagca cggttccggt acgacgccag ttctcgccca ttttcagctc
    56221 taagtcttca ccggcttccg gcagtcggct ggcgctaccc gtcagcaacc agagcgcgcg
    56281 tttgttggca ccacggaatt tagctcgcgc caccatctct tgtccggtat agcagccttt
    56341 tttaaagcta atgccgccca gcgcctggag gttggtcgcc tgtgggataa actgcccgct
    56401 gttggcggca tcaatcaccg ggaaacccgc ttcaatgttt aatgccagcc actgttggct
    56461 attgttcagt tccgcttcac cgcgcagttt atcggtcagc atattggcag tggcttcatc
    56521 ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat agcaaagtgg tcgcgccttc
    56581 tttaactacc tgtttttctt tcgaaggcag ttcactaaag atatttgcca gcgcggcgcg
    56641 cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg tctggcgcga tggtcacttt
    56701 agagaacacc gcatattttt tcagttcagt cagctgcggt tcacgcacgc tgcgccgttc
    56761 aatccacgca aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca ttttgccttt
    56821 ggcgtcgcaa tgggcggcga gcaggtgctg atcttctgtc atctggctga catctgctgt
    56881 cacctgaccc tgcatatatt tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc
    56941 atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc gtaggctgac ggggaggaaa
    57001 aggtgtaaaa gccataatag agtcctgagt tgcttaacgg cgtattaatg actaatggta
    57061 aaagagctat gaggcaatgc aagtgctttc actacgcgat ctgtgccttc tgatatgctt
    57121 tgcgaggcga cttccaaaaa aatgaagccc ttgaattgtt gcgaattaat ttcgcgatcg
    57181 cgattgtgtt tactgatatt cccgcaaaag aagtcgaaaa acacgttaca atagccgggt
    57241 actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg cattcattgg
    57301 gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt cgaacatgag
    57361 tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga atgcgacgac
    57421 ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga actggaaatg
    57481 atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg
    57541 cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg ccgctgttat
    57601 tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttgc tttcgctggt
    57661 ggtgtttgat tgcgttcgca gccagcgccg cattaatgct cgccaggggg aaattcgcct
    57721 gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca aagcgccgtg
    57781 gatgattaag agcggcatga tgctgcgttt acgttctgac agcggtaaac ggcaacattt
    57841 atggctggca gccgacagca tggacgaagc tgaatggcgg gatttacggc ggattttgtt
    57901 gcaacaagag acgcaaagat aaagatgacg gcaaagcata tgcgctaagc cgtcagtgac
    57961 atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc tgaatacgct
    58021 cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac agttgcccgt
    58081 cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag tagccgacga
    58141 acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg aggaaccact
    58201 cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct tcgaggttca
    58261 ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcgcca aaatcccagg
    58321 tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag tcgtccttga
    58381 ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct gccgccattt
    58441 cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat
    58501 tctgctgtgg cttttgcggc tagtgtatca gaatcgcttt agtcacggca caataaggca
    58561 taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg cacgcatcga
    58621 gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt tgaacgctta
    58681 ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgtgggttga cgctggcgac
    58741 ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg ggtataaagc
    58801 caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc tttatcgcga
    58861 aaagtttcgt gaagatgatc ccagtgcgca tctcgcttca ccgaaattgc cccagcgttt
    58921 gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcaccat taattgatca
    58981 gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg gactgcgtgt
    59041 ctctgaactg gtcgggctga caatgagtga tatcagcctg cgtcagggcg tagtgcgggt
    59101 cattggtaaa ggcaacaaag agcgcctggt gccgttaggt gaagaggcgg tttactggct
    59161 ggaaacctat ctggaacatg ggcgtccgtg gctgttgaat ggtgtgtcaa ttgacgtgtt
    59221 gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc gcattaaaca
    59281 ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc
    59341 ttttgccact cacttattaa atcatggtgc ggatttacgc gtggtgcaga tgctactggg
    59401 ccacagcgat ctctccacca cgcaaattta tacgcatgtc gctaccgagc gtctgcgaca
    59461 acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacaggaa aatttatgaa
    59521 gaaaggtttt atgttattta ccttgttggc agcgttttca ggctttgttc aggctgatga
    59581 cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata ttcagcccgc
    59641 gcccgtagct ggcatgaaga cagttctgac taacagcggt gtgttgtaca tcaccgatga
    59701 tggtaaacat atcattcagg ggccaatgta tgacgttagt ggcacggctc cggtcaatgt
    59761 caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga tcgtttataa
    59821 agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg gttactgcca
    59881 caaactgcat gagcaaatgg cagactataa cgcgctgggg atcaccgtgc gttatcttgc
    59941 tttcccgcgc caggggctgg acagcgatgc agagaaagaa atgaaagcta tctggtgtgc
    60001 gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg caccagccag
    60061 ctgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg ttagcggtac
    60121 tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc cgaaagacat
    60181 gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg cgtgaaacaa
    60241 cagatacaac ttcgtcgccg agaagtcgat gaaacggccg acttgcccgc tgaattgcct
    60301 cccttgctgc gccgtttata tgccagccgg ggtgtgcgta gcgcgcagga actggaacgc
    60361 agtgttaaag gcatgctgcc ctggcagcaa ctgagcggcg tcgaaaaggc cgttgagatc
    60421 ctttacaacg cttttcgcga aggcacgcgg attattgtgg tcggtgattt cgacgccgac
    60481 ggtgccacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc
    60541 gactacttgg ttccaaaccg tttcgaagac ggttacggct taagcccgga agtggtcgat
    60601 caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat ttcctcccat
    60661 tcgggcgttg aacacgctcg ctcgttgggc attccggtta ttgttaccga tcaccatttg
    60721 ccgggcgaaa cattacccgc ggcggaagcg atcattaacc ctaacttgcg cgactgtaat
    60781 ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc
    60841 acctttttgc gcgatcaggg ctggtttgat gagcgcggcg tcgcaattcc taacctggca
    60901 gaactgctgg atctggtcgc gctgggaaca gtggcggacg tcgtgccgct ggacgccaat
    60961 aaccgcatcc tcacctggca ggggatgagt cgcatccgtg ccggaaagtg ccgtccaggg
    61021 attaaagcgc tgctggaagt ggcaaaccgt gatgcactaa aactcgccgc cagcgattta
    61081 ggttttgcgc tggggccacg tctcaatgct gccgggcgac tggacgatat gtccgtcggt
    61141 gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat
    61201 gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc agattgaagc cctgaccctg
    61261 tgcgagaaac tggagcgcag ccgcgacacg ttacccggcg ggctggcaat gtaccacccc
    61321 gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg ttttcaccgt
    61381 ccggttatcg cgtttgcgcc agcaggtgac ggtacgctga aaggttccgg tcgctccatt
    61441 caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc cggcatgatg
    61501 ctcaagtttg gcggccatgc gatggcggcg ggtttgtcgc tggaagagga taaattcgaa
    61561 ctctttcaac aacggtttgg cgagctggtt accgagtggc tggatccttc actattgcaa
    61621 ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgactatgga agtggcgcag
    61681 ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt tgacggtcat
    61741 ttccgtctgc tgcaacagcg gctggtgggc gagcgtcatt tgaaagtcat ggtcgagccg
    61801 gtcggcggcg gtccgctgct ggatggtatt gcttttaatg tcgataccgc cctctggccg
    61861 gataacggcg tgcgcgaagt gcaactgact tacaagctcg atatcaacga gtttcgcggc
    61921 aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca tcttctctat
    61981 aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga ctagctcaat
    62041 aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca ggacctcacg
    62101 gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag agcgtctgga
    62161 agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac gcgcacaggc
    62221 cctgggtaaa gagcgttctt ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca
    62281 ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg acgaagaaac
    62341 ctttaacgaa gccgttgctg aactcgacgc tctggaagaa aaactggcgc agcttgagtt
    62401 ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata ttcaggcggg
    62461 gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt atctgcgctg
    62521 ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg aagtggcggg
    62581 tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc tgcgtacaga
    62641 aactggcgtt caccgcctgg tgcgtaagag cccgtttgac tccggcggtc gtcgccacac
    62701 gtcgttcagc tccgcgtttg tttacccgga agttgatgac gatatcgata tcgaaatcaa
    62761 tccggcggat ctgcgcatcg acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa
    62821 ccgtaccgaa tctgcggtgc gtattactca catcccaacc gggatcgtga cccagtgcca
    62881 gaacgaccgt tcccagcaca agaacaaaga ccaggccatg aagcagatga aagcgaagct
    62941 ttatgaactg gagatgcaga agaaaaatgc cgagaagcag gcgatggaag acaataaatc
    63001 cgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc gcattaaaga
    63061 tctgcgcacc ggggtagaaa cccgcaacac gcaggccgtg ctggacggca gcctggatca
    63121 atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg aacaacacgc
    63181 acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct
    63241 ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat ttccgtcgcg atcatacctc
    63301 tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag cgctgaacat
    63361 cgaagttgcc gttgctggcc gcatgatgac ccgtcgtatt atgggtaaag cgtctttcgt
    63421 taccctgcag gatgtgggtg gccgcattca gctgtacgtt gcccgtgacg atctgccgga
    63481 aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc gatatcctcg gcgcgaaagg
    63541 taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc tgcgtctgct
    63601 gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc aggaagcgcg
    63661 ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgcaaca cctttaaagt
    63721 gcgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct ttatggaagt
    63781 tgaaacgccg atgatgcagg tgatccctgg cggtgccgct gcgcgtccgt tcatcaccca
    63841 ccataatgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt acctcaagcg
    63901 tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg
    63961 tatttccgta cgtcataacc cagagttcac catgatggaa ctctatatgg cttacgcaga
    64021 ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac aggatattct
    64081 cggtaagacg gaagtgacct acggcgacgt aacgctggac ttcggtaaac cgttcgaaaa
    64141 actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg cggatctgga
    64201 caacttcgac tctgcgaaag cgattgctga atctatcggc atccacgttg agaagagctg
    64261 gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac atctgatcca
    64321 gccgaccttc attactgaat atccggcaga agtttctcct ctggcgcgtc gtaacgacgt
    64381 taacccggaa atcacagacc gctttgagtt cttcattggt gggcgtgaaa tcggtaacgg
    64441 ctttagcgag ctgaatgacg cggaagatca ggcgcagcgc ttcctggatc aggttgccgc
    64501 gaaagatgca ggtgacgacg aagcgatgtt ctacgacgaa gattacgtca ccgcactgga
    64561 acacggctta ccgccgacag caggtctggg aattggtatc gaccgtatgg taatgctgtt
    64621 caccaacagt cataccatcc gcgacgttat tctgttcccg gcgatgcgtc cggtgaaata
    64681 agcattacgt tatgctcaca accccggcaa atgtcggggt tttttaattg tgctgcgcga
    64741 aagcagacaa caattttctt gcttcactat tggctgcctg catcaccatc cacggactga
    64801 acgcccacgg cgtggcatca ataccgtgta atacatctgc taaatcacac cattgataat
    64861 ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca aataccggac
    64921 acacttcatt ttccacaatg ccattcggat cggtggcgcg atagtagaag tcaggataga
    64981 tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg atcaccgcgt
    65041 cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac acgccaggcc
    65101 acgctttttt gctaagggcg cggcgggtaa ctaataattg ccctttggca ttaaacagcc
    65161 aactggagaa cgcgagatgt aagagagtgt ctgccgtgtg tgcggcatac ttttccagcg
    65221 tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc ataatttctc
    65281 acatgtaatt ctaatcactc atcattatag ataattgaag agtgaaatga ttaaccaagt
    65341 gagcgtttat cgacaaccgc ccgttttgag cggatgtcga caggtaaaaa ccatttaaaa
    65401 aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt cagaaatgac
    65461 aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta aaagattcat
    65521 attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata cgtactgtcc
    65581 tgacttttaa atctttatca gagacggatt ccttcaccag atcagcatga tgctgatagc
    65641 cattaaagaa gacgttcagc acaacggcgc taagggttgc gagcataatg ccgctatgca
    65701 gcaacggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc gtcggagtca
    65761 tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg gtgtagttac
    65821 agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcacc agaccagcgc
    65881 cgcccagcac aaattgcgga atggaggcga ccagcaccgc catttttggc accatgccaa
    65941 acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg cgcgtcacgc
    66001 taaccaggcc aacgttttga gaaaatgacg tatgggggaa gctgttaaac gttccgccta
    66061 tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgtgaa gagagtttac
    66121 gaccaactat ttcacccagt gccaggaaca tccccatcga ctcgataaac acgatgatta
    66181 acacggcagt catggtcaga atggaaacgg gatcaaaaat cggcatacca aacgacatcg
    66241 gggtgacaat cgcaaaccac gaggcatcat gtagcccgga taaattgact tcattcatca
    66301 tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg gacataaatc
    66361 ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag atacctaaat
    66421 aaacgggatt accatattgc ggattccctt tacctcctgc ggcccagtca atacccacct
    66481 gaatgatgct aagtccgata gaagtaataa ccacaccggt aaccagtggc gggaataaag
    66541 gcatcaagcg accgataagt ggcgctaata atgtggtgat aaaacccgcg gcgatagtgg
    66601 caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc attggtgtta
    66661 cagcggcgaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag cggccgatac
    66721 cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta atgagcatcg
    66781 caatagcttc ttttgagagg cccagtcggt cgccaatcat aagaggaaca gcgactgcac
    66841 ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct ggtgataata
    66901 tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt tgggaatcta
    66961 tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg tttacgttca
    67021 tgaaactgtc taaatacatg ctcgtggagt gccagttttg cgaggaaggg gagttagaga
    67081 caggaattgc ggaggtaatc acataatcgt ggttaaacca cgaaaatgag aagagaacct
    67141 gacaatggaa ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc
    67201 gcactctcgc atttaatcgt ttttatctgg atagcattct tttgatcggc aatgttgaaa
    67261 taatgaaagc tgcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta
    67321 aatcggccat gctacctaat acatgcccat ttattgcatc gcgaaatttg agaagacata
    67381 actcattaaa tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt
    67441 ataggctgtc atgcttgtga aattgcctgt gcgatggcac ataatcaaga aaactggccg
    67501 ctgagtcaca gtgactttcg accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat
    67561 ccggtggcct gccatcactg caacaatgcc ccttgcgtta cggcttgtcc ggttaatgct
    67621 ctgactttcc agcccgatag cgtacaactg gacgagcaaa aatgtattgg ttgtaaaaga
    67681 tgcgcaatcg cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc
    67741 gacctttgta accagcgcag ttccggcacg caagcctgta ttgaagtctg cccaacacag
    67801 gcgttacggc tgatggacga taaagggttg cagcagataa aggtggcccg ccagcgcaaa
    67861 acggcagcag gaaaagcgtc atcagacgct cagccatctc gcagtgcagc gttgctcccc
    67921 gttaactcgc gtaaaggcgc agataaaatt tcagcgagtg aacggaaaac ccactttggc
    67981 gaaatctatt acgggctgga tccacaacaa gcgacttatg agagtgaccg ctgtgtttat
    68041 tgcgccgaaa aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac
    68101 atccgtctgg tgcaggaagg aaagattatt gaagcggcag aactttgcca ccagaccagt
    68161 tccttgccag aaatctgcgg cagggtatgt ccacaggacc gtctttgtga aggcgcatgt
    68221 actttgaaag atcactctgg cgcagtctct atcgggaatc tggaacgcta cattaccgat
    68281 accgcgctgg cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tatcgaaaaa
    68341 gtggcggtga ttggcgcagg gcctgcaggg ttagggtgtg ctgatatttt ggcgcgcgca
    68401 ggagttcagg ttgatgtctt tgatcgccat ccagaaattg gcggtatgct gacttttggc
    68461 atacctcctt tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca
    68521 atgggaatcg actttcatct taactgtgaa attggccgcg atattacctt tagcgattta
    68581 acttctgaat atgatgcagt ttttatcggc gtggggactt acgggatgat gcgagcagat
    68641 ctgccgcatg aagatgcgcc cggtgtcatt caggcgttac cgttcctgac tgcccatacc
    68701 cgccagctca tggggttgcc ggagtctgaa gagtatccgc tgacggatgt ggaaggtaag
    68761 cgagtcgtgg tattgggcgg tggcgatacg accatggatt gtttgcggac ttccatccgc
    68821 ctcaatgccg ccagcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg
    68881 cgcaaagagg tggtcaatgc gcgcgaggaa ggtgtcgagt ttcagttcaa tgttcagccg
    68941 caatatatcg cttgtgacga agatgggcgt ttaactgcgg tgggcctgat tcgtaccgcc
    69001 atgggtgagc cggggccgga tggtcgccgt cgtcctcgtc cggtagcggg ttcagagttt
    69061 gaattgcccg ccgatgttct cattatggcc tttggtttcc aggcgcatgc catgccgtgg
    69121 ttgcagggca gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg
    69181 tatttaccta cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc tgttcatggc
    69241 gcggatctgg ttgtcactgc aatggccgca ggaaggcagg cggcgcgcga tatgttaact
    69301 ctgtttgata cgaaggcatc atgatgaaat cgttaattat cgttaatccg gctgactgta
    69361 ttggctgccg tacttgtgaa gtagcctgtg tggtcgctca cccttcagaa caggagttga
    69421 atgccgatgt ctttttaccc cggctgaagg tacagcgact ggacagcatc agtgcgccgg
    69481 tgatgtgcca tcagtgtgaa aacgcccctt gtgttagcgc ttgccccgtg ggggcgctga
    69541 cgatgggcga gcaggtggtg cagaccaatt ctgcccgttg tattggctgt cagagttgcg
    69601 tcagtgcgtg cccgtttgga atgatcacca ttcagtcgtt gccgggggat acccggcaac
    69661 aaatcgtgaa atgcgatctc tgtgaacagc gggaagaggg gccagcctgc gttgaatctt
    69721 gcccaacaca ggcgctgcag ttgcttactg aaagagaact caggcgagtc cgccagcagc
    69781 gtattgttgc cagcggtgag aatcctctgt gaaagaaatc tccgcgctga aaggcgcgga
    69841 ggatctatta gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca
    69901 ccgtaccaat attcagcttt tgccattcgc gtgcaaagac gcggcctacg accagtgtca
    69961 caaagccaag cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca
    70021 ccaggccagc cattgcgtca ataaaatcat tgaaatccag cttcgagacg ttacttaaca
    70081 tcagcaaacc tacgtacatc agagcggggg cagtggcgta accagggatc aaaaatgaca
    70141 gcggtgataa gaacagaatc agcaggaata acgcccccac tacggttgcg gttaaaccgg
    70201 ttttcccccc ggcggcggtt cctgccgctg attcgatata aaccgccgcg ggcgctgcgc
    70261 ccaccaggcc ggagaatatt gaacttactg agtcactggt cagggctttg ccgccgttga
    70321 tgatctggtt gtctttatcc aacaaattcg cctgaccggc gacggcacgg atggtgccag
    70381 tggcgtcgaa cactgcggtc atcaccaatg ccagtacact cggaagtaca gttggctgga
    70441 gtgcgcccat aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcaggcttg
    70501 gcatcgccac cagaccgtgg tacttcaccg ctgggtcaaa gattaagccg atgatcgaaa
    70561 ttgcaataat caccaacaag atcccgccgg gtacgcgaca cttctccaga ccgaagataa
    70621 ccgccagccc cagcaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg
    70681 gcaagccttc aatcgggttt ttgataacca ttcccacacc gttagcagca atcagcagca
    70741 gaaacaggcc gataccgata cctgtaccgt gagcaatccc catcggcaaa ttacgtaaga
    70801 tccaggtacg cacaccggtt acggaaatgg cggtgaagat gacgcccatc agaaataccg
    70861 cgcccagtgc gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaacg
    70921 ccgtcaagga aatcgcacaa ccaatcgcca ttggcaaatt ggcccacaac cccatcagca
    70981 acgagccgaa gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg
    71041 ctttgcccag cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta
    71101 agccagccag tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat
    71161 ccagcgcgcc ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga
    71221 tgtcctcacg aggatgttaa agttttatta ttagttgcgt tcgtacacca gacgaccatc
    71281 aacgtaggtg cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc
    71341 gactaaagaa acagagttgt catagcgcag ctgctgtagc ggagtggcag tgggttccat
    71401 caccacgaaa tcagcctctt tgccaggtaa aaagttgcca atcaaatcgt caaggcccag
    71461 agatttcgct ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc
    71521 ttgtaattgc aataccttgt aggcttcgtt cagcgtttgc agcatgttaa aagttgttcc
    71581 ggcaccgata tccgttccca tgcccacttt gactttcttc tgccatgctt ttttcaagtt
    71641 gaataagccg ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt
    71701 ttcgctgaga cgatcccact ctttttcttc gagatggacg cagtgagcaa agacacagtt
    71761 tttaccggtc agaccgtact gatggtaaac atccagataa ccatcatggt caggataaag
    71821 cgatttgacc caggcaattt catctttgtt ttcacagaga tgggtatgta cccacgtatc
    71881 cggatattct tctttcaggc gttgcgccat cgccatctgt tcaggagatg aggtaggggc
    71941 gaagcgtggc gtaatcgcat acaacagacg accatttttg tgccagcgtt caatcagttc
    72001 tttgctttgg tgatagctgc tttcggcagt gtcgagcaga taatccggtg cgtttcggtc
    72061 catcatcacc ttaccggcaa tcatacgcat attgatatga ctggcggctt caaacagcgc
    72121 atcaacggat tgtggatgaa cagtgccaaa caccagcgcc gtggtggttc cgttacgtaa
    72181 aagctgcttg atgaagaacg ccgacatctc gcgggcgtac tctaaatcct cataacgacg
    72241 ttcagtaggg aaggtgtgtt tattcaacca ctccagcaat tgttcaccat aggcccccac
    72301 catttcactt tgcggataat ggatatgtgt atcgacaaag cccggtacta tcagtttgcc
    72361 gcgatagtcg cgcacgcgaa tggtgtcagg aatttgatgc tttccgtctt cccattcgcc
    72421 aaaccattcc acttttccct gtttaatgag taataaacca tcctcaataa accgcagcgc
    72481 agaggcaatt tcttccgggt tatcgaccgt acgggtgaca tcaataaaac tgcctcgtac
    72541 cgcttttaac gtgtgttctc ctgacatcat tgactccttt actgttaatc catctcttcc
    72601 gctgaggtaa taccaggcag aacgttttcc tgtcggtagc caccagggag gataatattt
    72661 aataaaatcg cagttaaccc gccagcacaa atagggtttt caactaatac ataaatagag
    72721 gctggcaata ttttaaaaat ttcgggatcg taggagacgc caagccctaa acctaaagac
    72781 gtggcgacaa taagtgtttc tcggcgcttt aatccgttgg tgatgatgat gcgaatccca
    72841 gcgatggcaa tcatggaaaa catcaacgtc attgcgcctc ccagaactgc cgagggaatg
    72901 gtcgtgaaga agccgccaat catcggaaat aagccgagga taaccagcat taccgcgatg
    72961 gttcgcccga cataacgtga agcgacgcca gtcatctgaa taaccccatt attttgcgca
    73021 aacgtggtta atggtaatga accgacagcg gaggcgataa cagaaaccag accatctgcc
    73081 agcacgccgc ctttcagtcg ggactgatac tcttccccct gaatggggcg gcgggaaacc
    73141 attgccgtgg cggtgatatc gccgactgct tccagcacgc taagcagata aatcgtgcca
    73201 accaccagga actgatggaa gctaaaacta aagccgtatt tgaacggatg cgggatggtg
    73261 attaacggca aattgcgcat actgctgaaa tccaccatgc ccaggcataa cgatgcgata
    73321 tagccgacac atagcccaat ggcgatccct cccatgcgta gcaacggact gcgacagcag
    73381 ttaaagccga tcaccacaat taaaaccaat aaaccaacgc cgagatgttc gtaattgccg
    73441 aacgtaccgc tgcttttggc tgcaaatcct ccaccaaaat cgataatgcc gactttaatc
    73501 aggcttaagc cgatcatcag taccacaata ccgctgacgg taggcgtaat aacccgacgt
    73561 aaatagggca ggataaaaga agagccgaca accagaaatg cgccaacgaa ggagacgccg
    73621 aggagcgacg acatgattaa ctcttcgtga aaaccgtcgc ttttcatgct gctgcccagc
    73681 gcaatcatga ccgtaacaaa tgaaaaattg actgactgaa ttgagagaag accagaaccg
    73741 acgatgccgt agcggtttac ttgtaaccag gtaccaatac cagaggcgat catcgccata
    73801 gaaacaagat aggcagttgt ttcagcggaa agctgtaagg tcgcacccac gattaacgcg
    73861 ggggttacca tcggaacgaa aattgccaac agatgggtaa tggcacctac gagagcctga
    73921 tgaaagggag ggcgatcctc cagttcaaat ataaggtcag aacctgcatg gtttatatca
    73981 gacatccctt cactcctttt aaagtaaatt tgatcatctg aattagtaaa ataaattccc
    74041 acgaatttgg ggcgcaggac gccttgagaa cggcttatct cattttcgca gtcactattt
    74101 agtcatcgtt caaacagaga aacaggccgc cgaagcggcc atttattact tatatttttt
    74161 ccagcgcggt gagtattttc tccggtgtga aatgccattc gcgtaaccag atgccgcagg
    74221 catcgtgaat tgcggtagca atcgccggag cggcgccatt tacaccgatt tccgagatcg
    74281 atttcgcccc gaatgggccg actttatcgt cgctcggcac cagcacagcg cggaaatcac
    74341 gcggaatgtc accaattttc ggtgcgccgt aactgcgtaa atcacgcgtt aacgggtgac
    74401 cttcggcgtc ataaatgatc tcttcgctca tactgtggcc gatagctcgc agagtggcac
    74461 cgtagatttg tcccaacgct aactctggat tgaccggtgt accgcagtcc agcaaggcgt
    74521 agaatttatc caggcggatt tcacccgtac gcgtgttgac ggcaacttca gcgaagtttg
    74581 cgccatacgg gaaggcgaaa tcaggcgtga tataactgcc agttcccacc agtgaaccaa
    74641 agccggtgcc ggtttcgcct ttatgggcta tctctccgaa actaacttcg cctttcttgc
    74701 cgcgtacgac gcccggcgtt gctagttgga catctgccac tggctcaccc aacatttgcg
    74761 caccgtggaa cagaattttc tcccgcagat tttctgctgc caaacgcgcc gcgttacccg
    74821 agaagcaagt accggacgag gcatatgcgc ctttatcaaa caacgcatga tcggtatcac
    74881 cggagataac atgcacgtcc tgcggtgggc agtgcagcac ttctgctgcc agtttcgtca
    74941 ccacagtatc cagaccagta ccaatatccg caccgccaga atgaacgata aaggtaccgt
    75001 ctgattccag tttgatcatg cagttagcct gatcgatatc tgggatccct gatttctgca
    75061 tgataatggc gacgccacga ccgatgtgcc agtcaccgtt ttgtggtttt ggtgaagacc
    75121 attggatcat ctcgcgaccc tgacgcagga tctcttccag tgcgcagctg gcggcggaag
    75181 gaacggaggt cggcgcttta ccttcaccga ttgcaccgag aattttcagc tcttgccctt
    75241 cgtgtacccg gttacgttcg ataatttcca gttggtcgat ctgtaactgt tcagccagtt
    75301 ccgctaatgc catggtgata gcgaagttac ctttcggtgc gccataaccc tgataagcac
    75361 catttgggca aatgttgctg tagtaggtgg tgacctggaa atcgacgtta tcgcacggat
    75421 ataacggcag cgacagcgcc ggtccgttac atggtacggt gagtgagtgg ttgccgtaag
    75481 ggccagtgtt ggcgcggaaa tccatcttca ctgctgtcag gcgaccatct cttttcgctc
    75541 ccagtttgac ggtgactttc gcaacgtgac gagaggtgtt agcaataaac tcttcttcac
    75601 gggtgtagcg gaacagtacc ggacgcccgg tcacgcaggt tgcccaggcg cacacttctt
    75661 ccagcaggat gtcctgttta gacccaaaac cgccgccaac tcgctcttta atgacatgaa
    75721 ctttatgttg tttcatgccc acgaggcgcg cgacctggcg gcgtaagtgc catggtacct
    75781 gggtggaggc gtggatcacc agacgatcgc cgtccatacg ggtaaagcag atatgtgttt
    75841 cagtcgggca ctgctgtgcc tgcgttgagt tataggttcg ctcaatgatc acatcggcat
    75901 cggcaaagcc tttgtccata tcaccaatat gaccatgaat actggcagcg atatttttgc
    75961 gtgggcgaga accgatcggg aagttgatga tcatatgctc gccgcgctgg gctgcatgac
    76021 agttatcttc ctccagagta tctggtgcac cagcaacata caccaccggt tcatcgtgca
    76081 cgacaggcgc atcttccgcc attgcctcgt caatcgacat taccggctta agcacttcat
    76141 attcaacgtc gatgagcttc agtgcttcga gcgcaatgtc ttcactttca gcgacgaccg
    76201 cagcaacgcg atcgccgacg tgacgcattt tcttgccgaa cattcggcgg tcaagcggtg
    76261 acggttccgg tgcgctctga ccacccgggg tatagtaaat atccgggcaa ttcaggtgag
    76321 taataacgtg aacgacgccc ggtaaggctt cagctttgct gacatccaga tgagtaatca
    76381 gtgcgtgagc gtgtgggcta cgtaacattt taatgacgca ggcgtcagcc gttacgcgat
    76441 cttcaacata gcagggtttc gcctgcacca ttttcgcggc atcagtttta ggataatgct
    76501 tgccaatgac ttcaaggtcg tcacggaaag tcggagcgat atcaatggtg gcctgcggat
    76561 tatttttacg tgcaaccgcc agttcaatga cctgatagta ttgctgccag ccagcatcgc
    76621 ggctaaacag acctgagagc gcgtcgtcga tctcttcgcg ggtaggtgcg tcgatgcgat
    76681 cgagaagatc ggtgataatc agagccgcag ctggatcgtt ataaccagac tgcaccacgc
    76741 caacatcaac catggcctgt tgaaccagac ttaactcatt ccatttgccc agagattctg
    76801 cggtacgaat atctgccttc tctaactgtg cggcgataag caaggacgcg ttaacgatat
    76861 taccgttaaa gataattgcg tcagaaccgg caaacccaaa gccatcatcg ctgttgcgta
    76921 cagagtgcat tcccatgtta aacaacagct tttgcacgtt ttcaccagga ttaacggtta
    76981 gctcctgggg cgtgccattt aaagtaaagt ggatgatcat acggcttcct ccccagcctg
    77041 ttggcagtcg gcataaaggt cggctaccag aactcccgtg atatagcgtt tataggccac
    77101 gctgccccgc aaatcttcct gcgggaaaat ggcgttggcg acagcctgtt caagggcatc
    77161 accttccagg ttttgttttt caacatcgtg cagacgcagt gctttactgg ctacgccatc
    77221 cagcgcaatt cgcataccgt cgtggtctgt tattgcaacg gctgccgtca cgacggttaa
    77281 acctgcctga gaacggctaa ttttgcgagt cgcacaggtg cgatacggat ctttaatgat
    77341 gatttcggtt agcaggcgat cgcatgggca ggccaggtac tcctcaattg acagagtttc
    77401 gccgttgcca aaaaccagtt cagcatccag tgccagcagg acgggaagca gcactgactc
    77461 ttcttgccgg gcggcaattt caccaccaat ggtcgactga ttacgaacat ggcgtgagta
    77521 aacaaaaccg agagcttcac gcagtgctgc aggaataaat cgcgcatcac gcagtgtttg
    77581 caagcgagac atcgcgccaa tccgcagtgc accgttatcc cagtcaaccc agtccagttc
    77641 cagatcctgt aaggaaatgg caatcttttt atcggtacgg gttggtgtag cgttgagttt
    77701 gctgcccccg gcgaaccaga cggcttcatc ctggtagcgg cgcttcagtt ccagcgcctg
    77761 ttcgacagag tcgggtctga aaaattgttc aatcatcgtc tttccttaat ttcccctctt
    77821 ctctgaaaga agaggggagc tgttatttat gccagcgcat ccatccgacg ccacatactg
    77881 gcagcggctt ttctggcttg cgcataaatg gaatcgcaat cgaagttaaa ctgacggtct
    77941 tcatagacca tcacaccatt gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa
    78001 gcgatatgcc cggcgatatt gtctgccagc agcggcgtcg gcgagttgta atcgcaaatg
    78061 gttaaatcgg ctttgtaacc ggcttccaga aggccaaatt tcgcgccaaa gttgcggctc
    78121 atcagttcgt tgccgttagc cagagctttg gcaaagctgt caggccacag cggaccaccc
    78181 gcatcgcgat gtttaaagaa ggcaaatttc atctcttcaa acatatctga accaataccg
    78241 tccgtaccca acgccagatt gcggatgtcg caaagatgat ggttgtagcc gacatggttg
    78301 ttcatgtttg aacgggcgtt atgcaccagg aatgcatcac gctgattgag caacgcgatg
    78361 tcgtctttag acagataaag cccatgagcg accagcgttt tgctgtcgat gagatcgaat
    78421 tgcgctaatc gtgccagcag atctttgccg taccagtgat gactatggga aacatcgtaa
    78481 aggtcttccg cagcgtgaat atgtaaacca cggcctgtgg ctttcacggc ctcacgcagc
    78541 atttccagac cggcatccgg cacagtaaac ggcgcgtgag caccaatatg agcctctacc
    78601 agatacggtt cagttgccgc tttcttcgca tcatcaatct gacgggcgaa gcggatgttt
    78661 tcttctacac cttcctgcaa ctctttgata ccgttgttac ggtcagtagt ttcaaaacag
    78721 gtcatcgcgc gcaggccaac ttttaaaaat gcgttgcgca atgtggagag cgacccgtcg
    78781 atatacgccg gagaggcatg gtgatcgata accgatgtac atccgccctt aatcgcttcc
    78841 agggaacaaa tcagtccgct gtaatagagc gactcttcat caagggcgcg atcgagtcgc
    78901 caccagagat ttttcagcgt tgagatgaaa tccgggcagg gggcgatgtt tgccataatt
    78961 ccgcgggaca gccccgagta aaaatggttg tgcgagcaga caattcctgg catcacaatc
    79021 cgaccatgca tctctttgta gctggcatcg gggtagcgtt gcgtcagggc atcgccgata
    79081 gcgacaatca catcattttc gatggcgata tcaacgcctt cctgcacttt cgctgggtgt
    79141 aactgcactg cggtgacatt cttcagaatc aacatgatta cacctccaca cggcccagca
    79201 gataatgatg atgctggtgg acatggctaa tgatgcggca catatcgttc agctccggtg
    79261 gtacgttgtt gaactgacct tcgttgtcga tgtttaacac ccagctttgg ttattcagac
    79321 gtacgcgtac ccggcaatct tccacaagga agcctgggtt gctgctgtta tcaaagtctt
    79381 gcgacaggct gaagacggtg attttgtctt tgtacggttt accgttccac gggcagaatt
    79441 gggcgcagtt gccacattcg ttacagtaag cgtcgaggtg cagcgtctgg aaacggttct
    79501 ggaagcctgg gaccgcaatg gatacgttgg cgcggttcgg gcagacatcc acacacttgc
    79561 tgcaaacgta gttacattca aggcagcgtg cggcttcctg ggcgacaaac gcgtcacgat
    79621 cgtcgctatc caccagggtg acagagatat cgcctttgcg ttgatagatt tccgccggat
    79681 taacgttgtt ccagtattta tcgttctggt gggaacggat attttcccga ctaaggatgg
    79741 catcggtcgc ccgacgcgcg gttccgacag cagcgacaat ggaggatggt ccgcgctgta
    79801 cgtcgccgat cataaagacg tcagtcagac gagtttcgcc attatggtcg acgtctggcc
    79861 agccgttttt gtccagcggc acgcccatcg cattcagggc ttcagtatcc tgctgttcac
    79921 caatggcggt gatcaggctg tctacatgca gtgttactgt ttcgttggtt tcaaccggac
    79981 ggcgacgacc tttctcatca ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat
    80041 cagcatcgaa acgttccgga ttattcagaa aacggaactc cacgccgtcg tgcaacgctt
    80101 cttcatactc ttcgcgccat gctggcatct cttgcagtga acgacggtaa acgaccgttg
    80161 ctttttctac gcctggaacg cgtaacgccg cacgagcgca gtccattgcg gtgttacccg
    80221 ccccgacaac gaccacatgt ttgcccagct tgagctctgt gcccttgttg tattcacgca
    80281 ggaaggggag tgatttccag acattttggt tgtcgcccgc cagtttcaca ccgctatttt
    80341 tatcagtgcc ggtggcaatc agaacatagt ggaagccctg attttttaac tgttcaacgg
    80401 tcagatcggg tgagcagcca tactcaaatt tcacgccgtg agcggcaaca aaatcgatat
    80461 cgtgctgaat taactctgga ggaatacgga actgaggaat gatatttttc accacgccgc
    80521 ccgcgttggc ttcgcgttca aacagcgtaa ccggatggcc cgctctggca aggaagtaac
    80581 ctgcggccag acccgccgga cctgcaccaa tcacggcaac cggatggcgt gaaccagaac
    80641 cggctggttt gtgccagcgt tgcttatatt catcccaacc tttttccagc gcgacttttt
    80701 tcagttcgcg gatattcagc gcactgtcgt aatccaggcg ggtacagttg tattggcact
    80761 ggtgatcgca aatatgaccg gtaatggcgg gcagggcgtt gcgttgatag atgagttcca
    80821 gcgcgtcggc atagcggtgt tcgccaagca gacggatgta ttccggaata tcttgcttga
    80881 tagcgcaggc agtaacacag ggggcaacgt agcaatcggt cagcggcagg tcttcagcca
    80941 cttcaatacg ctcttctggc ttccagtgtt tctgggtgta ttccatggtt aacgcgtctg
    81001 ctgccagtct gttcagtcgt tcgacgtcaa cgtggtcaag tccccaggcg tcggagcctt
    81061 ccagctcgcg catgcaggca cttaagcgca gatagccgcc aggtttcagc aggtcggttg
    81121 ccatagtaat agggcgaata ccagtatcaa aaatatcgcg gatagtcagc tgactggcac
    81181 caccggaata agaaatgggc agtttgccgt caaaggcgcg agagagaact gctgcaacgt
    81241 tgatggagag cgggaacagc gcacggcctg acatatacat ctcttcaccg ggcagtgcgc
    81301 ctttgttatt aatggtgccg agagtgttag tcagttttac gccaaagccg agtgattttt
    81361 cttttgccag tgccatcagg cgttccagca tttccagtgc ttgcgtcagc ttgaggtcgt
    81421 gatcaaatga ctcttctttt aagccgatgt aaccgaaacc gcagacatcg aggatctcac
    81481 gaacacgcgc gtaccccagt aaggtcgggt tgagtttcac aaaggtgttg agcccttttt
    81541 cttccagcat gtagcggcaa atggcttcga tttcatgcgg aggacagccg tgcatggtgg
    81601 agagggtaac cccttgtacc atgctggtgg ggatgcgagc gggtaaggct tgcaagcttt
    81661 cgcgtttttc ctgtaagccg tggcgggcta aaaatgcgtc atcctggagt agcttattaa
    81721 gtgcatcgcg gtattgagcg aatttcggat ggtcagatgc gtccatcata ttgtcgatga
    81781 actgctgcat cggcggttgc ttaatacctt cgaggttgta accgacgctc atattaaaga
    81841 tgaacgattt acaggaatca gaaggctgga acatcgcttc gagaaggtgc agggcaaacc
    81901 aggctttgag gtattcatcc caggctttaa gcagggtaaa ttcggtagac cattcggtgt
    81961 taaagcactc gtcttcggca tcgatacagg gcttttccag ctccaggcgg tcaagaattt
    82021 ggacggtttt tagttcgatg aagcgtccgc cagtcagcca ggaagtgaca atattttgcg
    82081 cgagctgcgt gtgcgggcca gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga
    82141 cgctaacagt tttacctttt acgggtgagt aaaactgttg ctcgggaata ccaaagattg
    82201 agcgttgttg ttggtattca tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac
    82261 gcataatatc ccccataacc ctctccttag cttatacaga tatcattgag tgaccggtgt
    82321 tgagaaatcc ggtggtttag ggggatagag caacaatcgc accactcttg ccaggtttgg
    82381 cgggaaatat aaaaaaatgt gaggcggctc gaaacttaat ggctttttgt gaatgaactc
    82441 acaggtgcca aatgagaatt aaaatcactc ctgaaaatta ttattgccag cccatttatc
    82501 actttttctc ttttctcaaa atcagtgatt ccttatcatt atgataaaga gtgctggagg
    82561 gattaaatat ttgaacgcaa tcgtttaact acgttttcta aatttcagta taccttttct
    82621 tcgctgtaat aaagtcgtca ggagtatcaa tatctaaaat tatttctgca ttttctattt
    82681 cgacggaata atgttctccc ataaggagtg cctgacgcat attggtgaca ttgggtcgct
    82741 ggattgcctg catcaggcat ggttttgaca ctaaaatcgg atggccgggg atgccattat
    82801 ggagtggcag tattgcgcca tcatttcgta acgaccagat ttttctaaaa atatcgatgg
    82861 tgagggttgg catatctccg tgggtgagaa aacaatgttc tgtttgcacc gcgggtactg
    82921 cggcctttac tgatgtcagt aaaccctgcg cataatctgg gttgtggata atagtgatat
    82981 tgctctggtt cgcataacgt tcgtgcagtt cattaccacg atagccggtg actaaaataa
    83041 ttcggctaca aaactgcaac gcatttttga tacttgtatc aagaattgtt ccctgttgcc
    83101 agggtaacat aattttccat tgccccattc ttgatgataa tcctgcggcg gtaattatac
    83161 agtcgatggc tgacatagtg attccattat ttacaggagc taataacgcc tgataattgc
    83221 gttacttcct gatggattaa gttgagggta atttatccca gagaggtcca taaagacgcg
    83281 tgaaaagtat aattgaccca tcggcattat tcattgattt aggcgcgcag aaacgtccca
    83341 ctgtgatttc tgttgttggt gctgggggga aaaccagctt gcttttttgg ctggcagaac
    83401 tgttccaggc gagcggcagg cgtgtattaa tcactacgac gacacatatg tttatgccaa
    83461 catctcactg gcccgtggtt ttctgtcgtg atcccgccat gcttcctcat gcgtctttta
    83521 tatcccccat ttcattttgt tttcacagct ggaaagcgaa ccagggaaaa gtgcagggat
    83581 ttacgccaga agcgattgat gcactggtac aacgatcaga gtgtgacgta attctcattg
    83641 aggcagatgg ctcgcgtgga atgccgttaa aagcgcctga tgagcacgaa ccttgcatac
    83701 ctaaaagcag ttgctgcgtg attgctgtga tgggaggaca tattttgggt gcgaaagtga
    83761 gcacagaaaa tgtccatcgc tggtcgcagt ttgctgatat taccgggtta acacctgatg
    83821 caaccttgca actgagcgat ctcgttgcgc tggttcgcca ccctcagggg gcgtttaaaa
    83881 acgtaccgca aggttgtcgg cgagtctggt tcattaaccg tttttctcaa tgtgagaatg
    83941 cgattgcgca aagcgagctc cttcaaccgc tgcaacaaca cgacgtagag gcaatctggc
    84001 tgggcgatat acaagaacat cctgcaatcg cgcgcagatt tgtgaattag cggcccgggt
    84061 attccgggtt actgaaagga tatccattga ggttggtatg aatattttca cagaggctgc
    84121 aaaactcgaa gagcaaaatt gtccgtttgc gatggcgcaa attgttgata gccgaggctc
    84181 aaccccccgc cattctgcac aaatgttagt gcgcgccgat ggctctatcg tcggtacaat
    84241 tggtggcgga atggttgagc ggaaggtgat tgaagagtcg cttcaggcat tgcaggaacg
    84301 taagccgcga ttattccatg gacgtatggc tcgtaacggt gcggatgctg tcgggtcaga
    84361 ttgtggaggt gcaatgtcag tatttatcag cgtccatggt atgcgtccac gtctggtgtt
    84421 gattggcgcg gggcatgtca accgggcgat agcccagagt gcggcgctat taggatttga
    84481 tatcgccgtt gccgatattt atcgcgaaag cctcaatcct gaactattcc caccatcaac
    84541 cacgcttctc catgctgagt cgtttggtgc ggcagtggaa gcactggata ttcgccctga
    84601 taattttgtc ctgattgcca cgaataatca ggatcgtgaa gccctcgaca aactcattga
    84661 acaacccatt gcatggctgg ggttgctggc aagtcgccgc aaggttcagc ttttcctgcg
    84721 ccaattgcgt gagaaaggcg tggctgaaga acatattgcc cgtttacatg cgcccgttgg
    84781 ttacaacata ggtgcggaaa cgccgcagga gatcgccatc agcgtgctgg cagaaatatt
    84841 acaggtgaaa aataacgcgc cgggtgggct gatgatgaag ccttctcatc cttccggaca
    84901 ccagctggtg gtgattcgtg gtgcggggga tatcgccagt ggtgtagcgc tacgtctgta
    84961 tcatgcgggt tttaaagtga tcatgctgga agtggaaaaa ccgacagtga ttcgttgtac
    85021 cgtggcattt gcccaggccg tgttcgatgg cgaaatgacg gtcgaaggcg tcaccgcccg
    85081 cctggcaacc agctctgcgg aggcgatgaa acttaccgaa tgcggattca tccctgtgat
    85141 agtagatccc gcctgttcat tgcttgatga actgaaaccg ctttgcgtgg tggacgctat
    85201 tctggcgaaa cagaatttgg ggacgcgggc agatatggca ccagtaacaa tcgcgcttgg
    85261 gccgggcttt actgcaggga aggattgtca tgcggtaatt gaaacaaatc gcgggcactg
    85321 gctcggtcag gtgatttact ctggttgtgc gcaggagaat accggtgttc ctggcaatat
    85381 tatggggcat accacccgac gggtgatccg tgctcctgct gcaggcatta tgcgatccaa
    85441 cgtgaaatta ggcgatctgg tgaaagaggg cgatgtgatt gcctggattg gtgagcatga
    85501 aattaaagca ccgttgacgg ggatggtgcg tggcttgttg aacgacggac tggcagtggt
    85561 cggtggtttt aaaattggtg atatcgatcc tcgtggtgaa acggctgatt tcaccagcgt
    85621 ttctgataaa gcccgggcga ttggcggcgg cgtacttgag gcgttaatga tgttgatgca
    85681 tcagggcgtt aaagcgacga aagaagtgct ggaagtggct taaatgaaaa tggccggatg
    85741 gtattaccca cccggccttt aaattactga caaatcaccg ttccggtttt gccttcaatc
    85801 ccttctttcg ctttgctcaa tacggtaatc agtgcttcac gacctgcgcg agagcgagca
    85861 aacgaggcgg cagcttccac tttaggcagc atagaacctt tcgcaaaatg accttcttcg
    85921 ataaagcgtt cagcatcact taatgacagg cgatcgagcc actgttcatt ctctttaccg
    85981 aagttaatgg ccactttttc taccgcagtc agaatgatca gcatatcggc atcaatcatt
    86041 tctgctaaac gggcgctggc ccagtcttta tcgataaccg cactggcacc gcgcagatgg
    86101 ttcccttcac gaataacggg aatgccacca ccgccaacgg tgatcactac ctggcctgct
    86161 tctaccagcg cttttaccgt ctctttttca ataatatcaa ccggtttcgg cgaggcaaca
    86221 acacggcgat agccgcgacc agcatcttct ttcagagtgt aaccctgttt tgtcagttgc
    86281 tctgcttcct gctcggtaaa gaacgaaccg atcggcttgg tcgggttgag gaatgccggg
    86341 tcgttagcat cgacttcgac ctgggtaacc agcgttgcta caggtttatt aatgccacga
    86401 gaaagcagtt cttctcttaa tgcgttttgt agatcgtaac caatataacc ctggcttaag
    86461 gcaacacaga cagacatcgg cagcattggc gagtgcgctt cagtttttgc ggcagcttca
    86521 aaggcctggt taatcatgcc cacctgtggc ccgttaccgt gagtgacgat aacttcatgt
    86581 ccctgggcaa ttaaatcaac aattgcctga gaagtaattt ttaccgcttt catttgtccg
    86641 gccaggtcat cgcccagcgc attcccgccc agggcgagaa caattttctt actcattttt
    86701 cctcactcat cattcttgat tgtgtataga tccctggcag tggttagagc acgggaggga
    86761 caaacggttt gcgtcgcagg aaacgacctc gcccggcttt gcctgtaaag gtgccgtcac
    86821 agaaaatcgt ttcaccacgg gataaagttc tgacaatcgc gccctgacag gtaaaaccct
    86881 cccatggcga gtagtcggcg ttgtcgtgga gatggcgatg ctgaatttgt tgactctgac
    86941 gtgggtcgat aatcaccacg tcgccatcgg aaccgggcgc taataatcct ttttgcggcc
    87001 acaggccaaa cagcctggcg ggcatcgcgc tggttaattc aacaaaacgt tccggtgtta
    87061 tacgtcccgt cataacgcca ctggaaaaca gtaactgcat gcggttttcc acaccgggta
    87121 agccatttgg gcagcgactg aaatcgcctt tagaaatttg caggcgttga gccatcgaga
    87181 atgtgcagtg atcggtcgct accacgtcaa tcgcaccatc gctgatgcca caccacagtt
    87241 tgtcctgctc gcgtacgtta cgcagcggtg ggctaagaat gaacttcatg ccatcttctg
    87301 tatcgtaact gcgttcgtcc aacaggagat attgtgggca ggtttcaacc cagactggct
    87361 ggtgattcgc acgggcaaga cgcagataat ccagacctaa gccgttagac aggtgcacga
    87421 tatagagcgg ggcgttaccg gcaatttgtg ccaggttaat catgcgggcg atggcttccg
    87481 cttcgcattc cagaggtcga ctcaaggcat gataacgcgg cgcggttaac cctgcggcga
    87541 taaactccgc ccgcttgctg gcgatagctg cgtcattttc cgggtgcacg gtggtcagcg
    87601 cgccggattc atgcaagcgg cgtaatgcct gcaacacttc atcatcgttg agtttatatt
    87661 gataggttaa atagagttta aaactgctca gtccttcctc aaccatcatc ggaatttcgt
    87721 cgaggatagc gtgattgata tgttgaatga caccgtgaaa gctgtagtcg atgactgctt
    87781 tgtgggcggc ataaccgcga taaacttcca gttgatggcg taaccggcag ccattcgggc
    87841 caaatcccat atggtcaata atggttgttg taccgccaca cgcagctgcg cgggtaccgg
    87901 taaaaaaatc atcacaactg cgcgcgatgc cgacatcaat attgaaatgc gtatggacat
    87961 ccacgccgcc agggaaaacg taacagccag tggcatcaat ttcttcatac gggacctgcg
    88021 gcgaaatatt gctgcccaat tggcgaacaa tcccgctctc aatcagcaaa tcttgtttgg
    88081 cttgtccatc tgcgttaacg acagtgccgt ttttgatcaa tacgcgcata gcaaactcca
    88141 aaaaccctcc ggcgaaccgg aggggaataa gtgaagagta attattcggt tgccagccag
    88201 cttaacggga ttgcagcgta catcgcggca caggtcacca ggtgagattt ccaggttttt
    88261 tcgttaggtg catgcgcttc aggttcttta cccgggccaa agccgatcac cggaatgccg
    88321 tgacggccca tgatagatac gccgttagtt gagaaggtcc acttatcaac aaccggcgct
    88381 ttgccaaaca gaccttcgta ggcattcacc agtgctttaa cggtgaagtg atcttcttcc
    88441 actttccagg tcgggaagta gcattcggtt gggtaaacca ggccagtcca ggacggacgg
    88501 tcgtagttgt acatagaaac aaccgcgtta gctttctgta ctgcaggcag ggcgcggatt
    88561 tcgtccagcg cgccttccca ggtttcgccc caggtcagac ggcggtcaat agagacggcg
    88621 cagctatctg ctacagcgca acggcttggg gatgtgaaga agatttcaga aacggtgagg
    88681 gtgcctttgc cgaggaattc gtcataaccc agacgttggg agagttcttg taattcgcca
    88741 agaatcggac ccattttgaa aatggcgttg tcaccgcgtt ctggtgcaga accgtggcag
    88801 ctcacaccct gaacatcaat acgaatttcc atacgaccgc gctgaccacg gtatacctgg
    88861 cagtcggttg gttcggtact gaccacaaat tccgggcgaa tgccggattg ttcaataatg
    88921 tactgccagc acaggccgtc gcagtcttct tcctgcacag taccggtaac cagcagggta
    88981 tattcatctt ccagaccgag gtctttaatg attttaccgg cataaaccat agatgccatg
    89041 ccgccttcct ggtcggaagt accgcgacca ccgatcagct catcagtttc catgccttcg
    89101 tacggatcga agtcccagtt tttgatgttg ccaatgccga cggtatcgat atgagcgtcc
    89161 attgccacca gacgcgggcc gtggccgata taaccgagaa cgttgcccat cgggtcgatt
    89221 tcaactttat cgaagccgac tttttccatc tcttctttaa tacgatgtac tacgcgtttc
    89281 tcgtcgcagc tttcactggg aatagcaacc atatcgcgca ggaagcgagt catgtctgcc
    89341 tggtaatctt ttgctttttc aagaatcagt ttgaatggaa tattcttagc cattattttt
    89401 ctccagattt tttactcccc gttttgcagg gagcagttcc aatagggtga ttaaggtgct
    89461 acagcgtgtt tgccttccca gacaacttcg cggtagtgct tcacgtcggt gtcgccttca
    89521 gtgctgataa ccagcaccac ggcatctttg ttcagcgcca gtttttccat caggctttga
    89581 cgttgcgggt gataatgaac cgctgcgaga acgcccaaac cgacagcgcc ggattcaccg
    89641 gagatgatgc gcgggtcgtt gccatacgga ttacccagca cgcgcatacc taatgcggca
    89701 acgctgtcct ggcaggagat gaattgggtg gcgcagttac gtaggatttc ccagcccagt
    89761 gggttaggtt cgccgcaggc caggcccgcc atgatggtgg ccatatcacc gccaacgttg
    89821 acgatgtcgc ctttgacgcc ggagcgataa atacagtcag ctttgtcagg ttcaacaata
    89881 atgctgtgca gattttgcgg gctatagacg tcgaccagat aacccagcac accaccggcc
    89941 attgcaccga caccggcttg cagcagaacg tgtgtcgggg tgacgcccat ttcacgcatt
    90001 tgctcgacgg cttcatccgc cagtgttgcg tagccttgca tgatccaggt tgggattttg
    90061 gtgtaacctt cccatgccgt gtcctgtacc acttcccagc cgtgctgctg cgcgtgttgc
    90121 atggtcaggc gaacggtatc gtcatagttc atatccgtga cgatgcactc ggcaccgagg
    90181 tttagaatgg cgtcaacgcg ttcctgagca gaacctttcg gcatgtaaat caccgcattc
    90241 tgtccgagtt gctgtgctgc ccatgccaca ccgcgcccgt ggttgccgtc ggtggtagtc
    90301 gcgaaagtca ttttttcgcc gatggcattt ttcaggtgct caaatgacag cgtttcgata
    90361 tcaagatgat atttttcaca caataattga gcgatggcgt acgcaccgcc aagcattttg
    90421 aaggcgttca gaccgaatcg ttttgattcg tctttgacga gaattttctt tacaccaaac
    90481 aggtttgcga gatcgtccag cgcacaaagc ggtgttgggt gataaccagc aattttctgg
    90541 tggaactggc gtgccagttt tgcctggctt tgcgaaaaaa gcggtgatgt ttcgccgttg
    90601 aaaaatttgt tatcggcgat atcaatcttc aatgagaaaa cggacatatc ctatcctttt
    90661 ggcctctgga aatagagggc cgaccagctg atcggcccga ggggttattt cacgcgttct
    90721 tgcgcttctt tcagcagttg ctcgagcagt gcacctggtt tggcgtattt acgggacagg
    90781 atcatcgcgg cgatgatata aggcttccag ctggcttctt tgtaggtagc gatacggtat
    90841 ttttcgaata cgccttcagt cacttcacct tctttgcagg atacgccgct gatatcagct
    90901 ggcaggcagt gcatgtacag ggcttcgcca tcacgggtca gttccatcat ctcttcagta
    90961 caatgccagt ctttgtgttg cgcgttctgt gccagacact gtttttccag tgcttttaag
    91021 ccttcgtgat cgttcgcacg cagcaattca gtacgctctt ccatcacttt gtaaggtgcc
    91081 catgacttcg gataaacgat gtctgcgtct ttgaaggctt cttccatgct ggtgacctga
    91141 cggaagctac caccggaggc tttagcattg tttttagcaa cttcaacgac atccgggatc
    91201 aggtcgtagc cttccggatg ggccagggtg acatccatac cgaagcgggt catcagaccg
    91261 atgatgcctt gtggtacaga gagcggtttg ccatagcttg gggagtaggc ccaggtcatg
    91321 gcgatttttt tgcctttcag gttttccagc gaaccaaagt gttcacgcaa ccatgccagg
    91381 tcagccatag actgagtcgg gtggtcaata tcgcattgca ggttcactaa agccggacgc
    91441 tgtggcagaa ccccttgttt gtagccgtca tcaagtgccg cgccaacttc acgcatatag
    91501 gcgttgcctg cgcccagata catatcgtcg cgaataccga tggcatcggc gcagaaggag
    91561 atcatattgg cggtttcacg cacggtttcg ccgtgagcga tttgtgattt gccttcgtcg
    91621 agatcttgct gtgcaaggcc gagcaggtta agcgcggaag cataagagaa gcgggtacgg
    91681 gtggagttgt cgcggaatac ggaaatacct aatccactat taaagacttt ggttgagatg
    91741 ttttcagcac gcagtgcttt taatgctgcg gcaacgtcca gtacttgttt cagttcatct
    91801 ggcgtctgtt cccacgttaa caaaaaatct ttctcgtgaa ggtgagaggt cagcgaattg
    91861 atatccttaa tcagctcatt aacagttttc atcataaagt cccggtagtg gaatgacagg
    91921 caaagtggtt tatttcactg tgacttgcca acgtgattgg tgttttcaca gctatacctg
    91981 ggactaacaa taagtgtgcc agaattatcc acggggctgc aaaagactaa tttgacggat
    92041 aatgtgtggg gttgatcaca aattgatgcc agcttctggt tgtttgtaaa atacgaaaac
    92101 gtttggcttt ttttatcgcg ttatttattt gctgctaagt atcagaaata agataaaggg
    92161 acaggcgatc tctcttcagt ttgataaact caccgccgta attctcatat tgatatagaa
    92221 atgtgatatc tcttccataa tttcattaga aagtgataaa aatcttatgt ttttatcaaa
    92281 gccaatttcg cctgagttaa tatcatcatg atctatgaac catcaacgtc ttcaatgatc
    92341 gacttttaag taagtctgga aggtaaagct atggagcttg ctactacgca gtcagtattg
    92401 atgcaaattc aaccgacaat tcagcgtttt gccagaatgc ttgccagcgt tttgcagctt
    92461 gaggttgaga tcgttgatga aaacttgtgt cgcgtcgccg gaacgggcgc gtatgggaag
    92521 tttcttggtc gccagttgag cggcaactca cgcctgctcc gccacgtcct ggaaacgaaa
    92581 actgaaaaag ttgtgacaca gtctcgcttc gatccccttt gtgaaggttg cgatagtaaa
    92641 gaaaattgcc gcgaaaaagc atttctgggt acgcctgtca ttttacagga tcgttgtgtt
    92701 ggggtgataa gtttgattgc cgttacccac gagcaacaag aacatatcag tgataattta
    92761 cgcgaatttt ctgattacgt tcgccatata tccaccattt ttgtttcgaa acttctggag
    92821 gatcaggggc caggagataa catcagtaaa atattcgcga ccatgatcga taatatggat
    92881 cagggcgttt tagttgttga tgacgaaaat cgggttcagt ttgttaatca gactgcctta
    92941 aaaacactcg gtgttgtgca aaataatatt attgggaaac ctgttcgttt cagaccatta
    93001 acatttgaga gtaattttac ccatggacat atgcagcata ttgtttcgtg ggacgataaa
    93061 agtgaattaa tcattggtca attgcataac attcagggcc gacaattatt tttaatggcg
    93121 tttcaccaat cgcataccag tttttctgta gcaaatgcac ctgatgaacc gcatattgaa
    93181 caattggttg gcgagtgccg tgttatgcgg caattaaaac gactcattag ccgtattgca
    93241 cccagcccat ccagcgttat ggtggttggc gaaagcggca cgggtaaaga agtcgtcgcc
    93301 cgtgcaatcc ataagttgag cggaagacgg aataaacctt ttattgctat caactgtgcc
    93361 gcgattccgg agcagcttct ggagagcgaa ctgttcggtt atgttaaagg cgcatttact
    93421 ggcgcttctg ccaacggtaa aacagggttg attcaggcgg cgaatacggg cacgctgttt
    93481 ctcgatgaaa taggtgatat gccattaatg ttgcaggcta aattactgcg cgctattgag
    93541 gcacgtgaaa ttctgccgat tggtgccagt agcccaatac aagtcgacat tcgcatcatt
    93601 tctgcaacta accagaattt ggcccagttc atttccgaag gtaaattccg cgaagatctc
    93661 ttctaccgac ttaatgttat cccgataact ctgccaccgc tgcgtgaacg tcaggaagat
    93721 attgaactac tggtgcatta ctttttacat ctgcataccc gtcgtctggg atcggtttat
    93781 ccgggcattg ctcccgatgt cgtcgaaata ttgcgtaagc atcgttggcc cggaaacctg
    93841 cgcgagttaa gcaatttgat ggaatatctg gttaacgtgg ttccttcagg tgaagttatc
    93901 gacagcacgc tattgccgcc aaatctgctg aataatggca caacggagca aagtgatgta
    93961 acagaggtca gtgaggcgca cctggcactc gatgatgcgg gcggcacggc gctggaggag
    94021 atggaaaagc aaatgatccg cgaggcgctt tcacgtcata acagcaagaa ggaagttgct
    94081 gatgaactgg gcatcggcat tgctacgctc tatcgcaaga ttaagaaata tgagttgtta
    94141 aacacataac ctcagtgaat ccggcctgaa ttcaggccgg atatcctttt acttcgtttt
    94201 ctcgcaatcc agaactgtat ttacaatcat ctgatacccc gtgcagcgac aaagatttcc
    94261 cgccagtcca cgacgaattc ccgtaatggt taatggcttc tcgcgtggtt tcgccagcat
    94321 tgccgtggta gccataatca ggcccggcgt acaaaacccg cactgcactg cgccggattt
    94381 cgcataagcc tgctgaacat gagaaagttt tccgcctttc gcttcacctt ccagcgtgcg
    94441 gatctctttt ccttcagccc aggcggcaag gtataagcaa ctgtctattg ctgtgccgtc
    94501 gaccagcacc gtacaggcac cacattcacc cacgcagcac ccttgtttga cacttagcag
    94561 tccttgttcg cggagtaatt ccgagagcgg cgtgcctggt gcggcgtgaa gctgaaaagg
    94621 tatcccgtta atggtgcatt cgatggtaat tgtttcgctg tgattcattg caattttccc
    94681 cccgccgcgg cgacggcttc gctaatcact tttttggtca tcgtctggat gagatgcaga
    94741 cgaaactctt tactggcccg ccatgacgaa cgcggggcga catcttgcag gactgattcg
    94801 ctgatggctt ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg ttcggcatgt
    94861 tggcagcgaa ttggcgttgg cgcggcaaca ccaaatgcca ggcgtaattc gctgaaattg
    94921 ccgttatcca gttggcaatg tgcggcgcag ccaatcgttg aaatatccat tgcgtcgcgc
    94981 atggcatatt taaaatgcgc gctgcccgcg tgttctttcg gctggggcgg aaaatgaaag
    95041 gcgacaagga tttcgtcatg ctcaagagac actttgcccg gcccggtgtg aaagccatta
    95101 atcgggacga aacgaacacc gcgcggggag tggatctcca gtttcgcgtc ataaattagc
    95161 gttggcgtgg cagaatctgc gctggtggca ccgttgcaaa tatttccacc gtaggtagcg
    95221 acgttacgga tctgcggtcc ggcaatggat gaggccgcag cacataacgc cgggagatga
    95281 cgttgagtta caggatcttc aatgagctgg gtaaatgtcg ttgcagagcc gatacgtagt
    95341 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
    95401 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
    95461 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
    95521 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
    95581 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
    95641 caccgcgtta cgaatagcag cggcaacagg aattattggt ggctcaccca gtgacttatg
    95701 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
    95761 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttcgc
    95821 atcaatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
    95881 ctgaccttct gccagtagcg gattaagaat atggcccgaa tcatgaacgt tgaggatgcg
    95941 gttgatggtg actttacaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
    96001 cgctggtggg ttagtggtgg ttttgatgga gctttcggca gagagctgcc cgccgcgttc
    96061 agggtggtag aaggcgtcca tcgccaaatc ttttaacgac attaatggct cttccggtcg
    96121 ttcaaccagc acgatatggc cttttatcag ggtaagattc atcgctgact gatgtagcat
    96181 gacagcggca tgagcgatga ttttctcctt taataacagt gccgcactgc gcagcgcagg
    96241 cgcggcaaca tagctctggc gtgaggcaaa tgcaccggga tcgaacggcg taacgtcggt
    96301 atcttgggtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgctaccat
    96361 ttgcgagaag acggtgtcgg ccccctgacc gatttctgtc gcgccgcttt gcacgttgat
    96421 ggttccatcc tgattcatca acaggcgcgc gcctgctatt tctacgccga caggccaggt
    96481 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgtaaattgc cttgctggtt
    96541 ctggcactct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
    96601 cggcaaccct gcgctgtaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
    96661 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
    96721 catagactca acggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
    96781 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
    96841 agcgacttta tttccgcccg cagaagcgat ggagtgcccg tgagatgcat aagcgccggt
    96901 gttagacaga acatccagac tataaccttt caatgttccg tcgcggttca cgcccatttg
    96961 cccgtcaata gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt cacggctaag
    97021 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
    97081 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
    97141 tgaccaggga atattcagcg cctgaccaac cacacggcga acaatgtgcg ggatctgggt
    97201 gctggaaacg atggtaattc gcgagtcatc ctccatccat gccagcgatg tcacgctttc
    97261 catatgacaa tgttgaataa cgggagtctg atagtgcccc tgcacctggt aatcggcggc
    97321 atcgattgtt tgttggacat tacccgtcga catcgagctt tgttttagta aattgccgcc
    97381 gttatggatt ggtgcagcgt catctgccag cgccgcttct ggcgtggtga taacgggtaa
    97441 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcacgggc
    97501 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
    97561 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatgggat
    97621 atcaggcaca tcttcccagg taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
    97681 atcattaata cttatggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
    97741 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatggc
    97801 atctacgcgc atgcatgatt cacctgtagc ggttgcttcc cgcgcttcca tagaaatccc
    97861 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
    97921 cagaaacgac gataagcgtt aagggaggaa ggaagtgtga tgatgttaaa aaaacacaga
    97981 tatgatacga aacaggaccc tgaatagggg cagtgatacg tatcaatatg agtggcgata
    98041 aaatcaatgt gataaattgg gattattacg catcaggatg ggaaataggg taaatgagtc
    98101 agaaaattcg atacatctac agtgaatatt ttgctgatga tacttccctt tgccaaatga
    98161 aattaacctc tttctctatt accgcgtttt tccagaagca agagattggg tgtcaagagg
    98221 ctggctgcta tgatatggcg tcttgttttt taagcgagga aagattttga gtgcgggacg
    98281 cctgaataaa aaatctctgg gtatcgtgat gttgttatcg attggactgc ttttggcggg
    98341 ctgctcgggt agcaaatcat ccgatacagg aacgtattcc ggctccgttt acaccgtgaa
    98401 acggggggat acgctatatc gtatttcgcg caccacggga actagcgtaa aagagctggc
    98461 gcgactgaac ggcatttccc ccccttacac cattgaagtt ggtcagaaac taaaactggg
    98521 tggggcgaaa aatagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc
    98581 ggttacaccg tcatcagcag taccgaaatc ttcctggccg ccagtagggc aacgttgttg
    98641 gttatggcca acgacaggga aagttatcat gccgtattcg acagctgatg gcggcaataa
    98701 agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt
    98761 ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga
    98821 agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt
    98881 gaaggctggg caaaaaatcg ccactatggg gagcactgat gcggcatcgg ttcgcctgca
    98941 tttccagatt cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgcctcaggg
    99001 cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag
    99061 gagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa
    99121 cgagagcttc ccaagctcta tacgagggtt cgattccctt cgcccgctcc aaacaaacat
    99181 ctaccaacgt ctactaaagt ctgccaatcc cccgaatatc aaggcctcca gcgattttcc
    99241 tcgtctacaa gcgtctacag tcatctacct aaatctacac acagaagtgt atagtaatgc
    99301 gtataggaca aaatcgtttt ttgggggcta tacacatgtt gctttcagac cttgctatcc
    99361 gccgcgcgaa gccaaaagag aaagcctata cactcaatga cggcaacggg ctttcgttac
    99421 tcattgagcc taacggttcg aagggctggc gattgcgata caggtttgca ggtaagccca
    99481 aaatgttgtc gctgggtgtt tatcctgagg tgtccctttc tgatgccagg gcggcaaggg
    99541 atgaagccaa aaggcttatc gctggtggag ttaatccaag tgatgtgaga aaagcccata
    99601 agagagaaca ggcgagcaaa ttcggtaaca cctttgaggc gatcgcccgt gagtggtatg
    99661 aaaaacgcct ggacagatgg tcacaatcat atgccgcaga gatgattgat acgtttgaga
    99721 aggacgtatt cccgtatatc ggcagcaaac cgatagcaga aataaaaccg atggaattgc
    99781 tcgcggttct ctcccgtctt aatgaccgtg gcgcaactga aaaattgcgt aaggttcgcc
    99841 agcgatgtgg cgaagtgttc cggtacgcaa taattaccgg acgcgctgaa tataaccccg
    99901 ctcctgatct ggcaagcgca tttgcaccac acaaaaaaga gcattacgcg tatctgaaag
    99961 ttgatgagct accggaattt ttccgcgttt ttaatacgta caccggaagc cagatcgtca
   100021 agctgggtat gcgattattg attcttacag gactccgccc aggtgaattg cggcaggcag
   100081 agtggcagga gattgatttt gataatcgtc tgtgggaagt gccgaaagaa cgaatgaaaa
   100141 tgcgccgtcc tcattgcgtg ccgctttcag aacaggcgat agctattctg gaacaactga
   100201 aacacatgac ggggcagtat ccgtttattt tccctggtcg aatacagcac agtaaaccga
   100261 tgagtgaaat ggcgatgaac gtgcttattc gtcgtattgg ttatgcaggg cgtgttactg
   100321 gtcatgggtt ccggcacacc atgagtacga ttttgcacga gcaggggtat aactccgcat
   100381 ggatagaaac gcagctggcg cacgtcgata aaaactcgat ccgtggcaca tacaatcacg
   100441 cgcaatatct ggacggacgg cgggagatgc tccagtggta cgccgactat atggactcac
   100501 tggaacaggg cggaaacgtg gtacatggca agtttggaaa atgtggatga ctggttgagt
   100561 atacagtagt agactttgag cgactaaata acaggctgtg tctagggtcg ctcccgaaac
   100621 cccgtacacc tctgcggact ggcacagcca ccactacaga gagtgcgagg tgtgcacatg
   100681 ccggttgtga atgatttccc tgacttaagt cattggttta cagtgcagga gtttactatt
   100741 actcaagctg cgttattgct gtcaggtatt gatcctctcg actacccaac tctggagatg
   100801 gtcaaagagg aaaaacacga acgatggaga atggcatatg ggtttgctta tggaatggtt
   100861 actgcaattc gacgcggtgt cttaactccc gttctgtgcg ttgcgtatga ttggggggat
   100921 atgcgtcgtt taaccccagc agagatgatg gatcgttcaa ataatatatc catggacgga
   100981 acaattatta cccgttattc attacaggaa tgggtaaaaa atgagaaagt tgattatgta
   101041 agaccctctc gctctatatc ggataaggag attaagccat ctaaccaatc agtaataatt
   101101 gatgccgaat ctagcgtgat taattctgaa gaaacagtat tgctgactta tggtcataag
   101161 agcgaaggtc ttgaatatgt agaagacgca ataagagaac tttgggcaac atatgatcca
   101221 gataatcaac aaacagcccc tacaagaaat gatgttgttg aatacctgaa aggcagggga
   101281 gcggggcaga acatggccga ggctgtaaac ctgatactac gccccactgg cttgcagcaa
   101341 gttggaagaa ggaaaagaaa aacaaatagt taaatagtgc ccacccttcg taaaaaaggt
   101401 ggcactattt gctcaaaact cttttgttta tggcgagaaa tcatagttaa gcgtgcgttt
   101461 tctttgctta ttgatactgg aatggtggca gcattattaa aataatagtg ggcactattc
   101521 cattgaaata gtacccacca ttaaaaatag tatccaccta taaatatgtt ttccttttat
   101581 gcatcattgt agacggttat agacgttacg aggattgaat atgtcaaata acttacttcg
   101641 tctttctgat gttatgcgcc gtactggtta cggtcgcgct tggatttatc gcctgatcaa
   101701 ccaggggcgc tttcctaaac ctgtaaaaat tggtccccgc tccgtggctt ttattgaaag
   101761 cgaagtcgat gaatggatta accagcgtat cgaggcttct cgcaacaacg ccgcgtaata
   101821 atcttttggg agggggatat tctcccccag cactaaacct gatccgatag caggagtttt
   101881 tcaatgaaac gtccgaatat gcccgtacag gggcagggct tcgttcagcc cgaagaactg
   101941 agccaacagt atttcgccgc aattattccg gtaatttccg gcaatgagat tattgctgac
   102001 ggaataaata aaaatgcact caccgcgccg gaagtgtttc cttctggcgg tgagcctgta
   102061 accacttcac aggctatcgc ccgatttttc agaaaggatc acgataaggt tgttaaccgg
   102121 attgaaaacc tgtgttgttc tgctgaattt ttaacccgca attttcggcg ggtaaaattt
   102181 gagcataagg ggcgcgtatt gaaagcctac gaagtcacca aagacggctt cgtgttttta
   102241 acaatgggat ttaccggaaa aaaagcggct gcattcaaag aggcatacat tgcgcagttt
   102301 aaccgcatgg ctaactggat agcagagaga tgtgaggcca aagctgagat gcgctctatg
   102361 ggggatgcca tgaagggata cattgagcgc actggagata ctcaacacgg tcatgcttat
   102421 aacaacgaat gtactttcat tgcgcgcctt gtattaggga tgcacccgcc tgtatgggcc
   102481 aaaaaaaacg gcatcccatc aaaaaaagtt cgtgaccata tgaccgctga tcaactggcg
   102541 ttgctcgcgt atctggaaag ccgtaactgt gcgctactgg atatggatat gccaacgcca
   102601 gaacgtaaag aacgtctcac cgaattagcc caacgctggc tggtccgtcg cctgggagaa
   102661 cgtgaacatg cctgacatcc gcaacgctgc cgccctgtac atcgatactg cggataagtt
   102721 cgcacaggtg agcatcaacc atgctgatta catgcgccat atcgtgagcc atttcgccgc
   102781 tgagcgcatg aaacacgtta agcattatca gaaggaggct ttatcaaatt atcgcgctgg
   102841 tggccctgta cggcgtgaat atccgcatga tgcaggaatg gcaggcaaaa caaaggcgac
   102901 cacgaaaggg tcgccagtgg agataaagga aatcaagcgc atcaccaact atgccatgag
   102961 tgtagctggt ggacaatgct atcagcctgg cggtcggttt ttagtggctt ttaaccgccg
   103021 tgggtatttc tcgacaaaag cctcaagggc aaatcgtgtt ggcctggtga gtggcagtgg
   103081 attaagtaac aacctccttt gccgttgtgc tttttcgcgc tgtcgctcaa gtatggataa
   103141 tgcgatttcc tgtagttctt catttgtcat tgtctgtacc cgtaaagcgg tgcgctgtag
   103201 tacctcatat cacagcgcag gtatttttta tggttgtaat ttgcgtgatg attcaggttg
   103261 ctttgcgaca cactgcataa acataaataa cgtgatgtta ttatgtgctt tcaaagaaaa
   103321 cgcaggtatt tttcgcacaa acttttctcg caacacatcc gaccacttaa gcaaaaaagc
   103381 ggcatatact gaggttatcc cttataaaac ggggatcggg cgtaggaacc cgtttaactg
   103441 taaggcgata aatgacgcca gaagcgtctt tttttctgcc agcgctttac gccatatcaa
   103501 tggcgttgtg tattgtgcgt actcgtgcac catccagtca tttcacgagt tgcattttgc
   103561 acagcgccac cagcaaacaa tggtgatgct ggcggggcta ccgaaaggta ggccggagtc
   103621 cttacagtcc ggtattccta cccccgccag tgtcaccgcc cattgtgagc gtaggaactc
   103681 ctgcggtgac gttattcatc actgtaagga ggctgccaac atggctacta cccctaccct
   103741 cgtacattct cagactgcct ttatctggcg ctttatcatc tttggcgcgt cagaatccca
   103801 aatcatccac gtaaccgcct ggacggaacg cgaagcgcgt aaccgttgcc cgtcaggttg
   103861 cgttgctgta ttcgccgccc gtatccgtca ggaggcatca catggataaa aacatgttga
   103921 tcgcctcttc tttcgggctg tatgatgccg gagtcgccca catggcgacc gggtttagca
   103981 gcctgaatca gatagcggac agccgcttac attccgatat gcggtttttt tgtgtccgta
   104041 agcctaccca tacccgcatt atggcggggc gtaacggggg agcctttgcg ctcgctggtt
   104101 cctatctggc cagtctgcta accccgttac gtctcgccac catgtttagc agcgtagtag
   104161 cgagactcct taatcaacag ataggagcct ttcacatggc tgtatccgca cgcccttact
   104221 ttgtctggcg ctttatgcag tgcctgacag gcagcattgc aatattcacc gttaccgctg
   104281 caactgagcg cgaagcacgc gcccggctgc cgcacgcaca tctgattttt gtcgcccgaa
   104341 tccgtcagga ggtgactcat gcttaaaacc ttccgcgtct tttcccgtgc tgttaacaag
   104401 cgcggctata ccgttggcat cacgcagaac gtgaaggccg aaaacgtcca ggctgcaatc
   104461 agcacagtaa ttaacaaatc agctaaagcc ggtttatccg gcgtaattat ccacgcagtg
   104521 acatgtattc aggatgagga gtaattcatg tgtaattttt ccgatatgac tgttgatgaa
   104581 gcagcaaaaa gcgcaatgca ggcagaactt atttgttctc tgatgatggg taacacaggt
   104641 gaaatgactg agggtgagat tgagtcattg ctcgccctga ttaagcagct tacaggtaag
   104701 gctggtagct ggctgattgc ggcatcgggt gatatgcaat aagggaataa ataacaggat
   104761 gaaataaata ccagcgaatt aaagcgttac atatcaggaa ataaaacgat gaaacgtaaa
   104821 tattctggct taactgccag tagcaaaact caccccgaat ttatgtgcgg ggatacttat
   104881 cgcaatgaat acggtagcca catgacgatg atcagcagag gtaagaaatg aaaagcgcac
   104941 cgaacttaaa aaaacagcct cgagggaaaa aacacgccga tacggaagtg attattttcg
   105001 ctggcagtga tgcgtgggcg cacgcgaaac agtggctgga gcaggacgga aaacttgccg
   105061 gagataatat accgcccgtc gtgctggctg atgagcaact taaagatatt ggcaatctgc
   105121 aaattgtacc cgacgggcgc aaatcagcgc gtatatacaa ggccggacac ctcgatcagg
   105181 tgatggtcaa aggcataggt cagaagctgg cggcggcagg cgtacaggac gcagactatt
   105241 accccgaagg tatgcaccat aacgagcggc agaactggcg taactatctg gagactgagc
   105301 gcaaaaacat ttctgacggg ctggttattg agctgcctgt aaagaaaaaa gagcggggaa
   105361 aaggtgatga tgccacatca ctggcgctaa accagatggg ggcaagccag cgcggcgaag
   105421 ttcttctggc acattatggc ggcgaactgg caataaacgc cgactctgac acagtgcatt
   105481 attacaacag tgttgtatgg gttcccgtac aggataaaga gctacagcgc acaatggcgc
   105541 aaatcttcat tgacgaagat atctgctatt cacaaaacgc cataaaatcg gcggttgata
   105601 ccatgaagtt aagtttaccc gtaatgggga ataccgcccg caacctgatt ggattcagta
   105661 acggggtatt tgatacccgt acaggtgatt tccgtgaaca cgataaaaat gactggctgt
   105721 taattgccag tgaattaccg ttcaccccac cagcagaggg tgaaacgctg gcaacacatg
   105781 cgccgaattt ctggaaatgg ttgcgccgtt cggtggctga gaatgaccgc aaggcagatc
   105841 gcgttctggc agcgttattt atggtgctgg cgaaccggta cgactggcag ttattcattg
   105901 aggtaacagg tcctggtgga agcggtaaaa gtgttatggc agagatttgt accatgctgg
   105961 caggtaaggc caacacggta tcggcaagca tgaaggcact ggaggatgca agggaacgtg
   106021 cattagtggt tggcttttca ctgattattc tgccggatat gacccgctac gcgggtgatg
   106081 gtgcagggat aaaggccatt acgggcggtg acaaggtggc aattgacccg aaacacaaag
   106141 cgccatactc aacgcgtatt ccggcggttg tgctggcggt taacaacagc gccatgtcat
   106201 tcagtgaccg cagcgggggg atctcacgtc gtcgtgtgat attcaatttt tcggaggttg
   106261 taccggaaaa cgaacgcgat ccgatgctgc cggaaaaaat agaaggtgag cttgctgtag
   106321 tgattcgtca tctgcttaca cggtttgctg atcaggacga agccagacgc cttttatatg
   106381 agcagcagaa atcagaagaa gcactggcga taaagcgtga gggggattcg ctggtggact
   106441 tctgcggcta tctcatgtcg tcggtaatgt gtgacggcct gttagtcggt aatgctgaga
   106501 ttattccttt cagtccacgc cgttatcttt atcacgccta tctctcgtat atgagggcgc
   106561 acggtttcag taagcccgta acactgacgc gcttcggtgc agatatgcca ggcgcaatgg
   106621 cggaatatgg cagggagtat atgaaaaaga gaactaaaga aggtgtgcga tcaaacgtga
   106681 tactgacgga tgagtctgag gactggctgc cgtcatgtgc accagtgaaa aatgagggtg
   106741 ataaaaatta agttatagaa aaactgttca ccactgttca ccctgcaata aatgtattta
   106801 gtatcaatat gttaatgggt gaacactttt gtaaaactat tcaccaaagt attcacctgt
   106861 tcaccttttt gtatgatttt tgaacagtca cgggtgaaca gggtgaacag ttggtgaaca
   106921 cctttacaaa aagtgttcac cccttaactt tatgaatcta aaggtgaaat agcaaaaggt
   106981 gaacagttga acaccttttg ggtataaatt tttatttatt cctctgagaa aaattttatg
   107041 acagagaaac acgctaaaaa atcacactca cacgccatcg atttaaccga acagggatca
   107101 gcaaaagatc cgcacgggca atcgctcacc atttcatgac gctgtatgac ctgaaagcgg
   107161 aagatgcaga aaaagcggca tgtgatttat tcgatatgac aatggggatt atccaggagt
   107221 accggaacaa ggagcgcaga ccatgacagc acaaattgca gcttacgggc ggctggtgga
   107281 cgacccgcag gtaaaacaga ccagcaaggg aacagcgatg acgctggcgc gtatggcggt
   107341 atcactgcca tgcagtcagg cacaggacgg acaggcgacg ttatggctat cagtcctggc
   107401 atttggtaag caggccgacg cggtggcgag acatgaaaaa ggcgacctta tcagcgtggc
   107461 gggtaacatg cagatcagcc agtggaccgg acagaacgga gaaacacggc agggctggca
   107521 ggttatcgct gacagcgtaa tcagtgcccg ttcggcgcgt cctggcggga agcgggggca
   107581 acaggggaag gcgaccgaag cacaggaaag agcaaaacga cagaccggac agcagggaaa
   107641 tcagccacca gcgggaggtg atgacccgtt ctctgacgat attccgttct gaatgacaga
   107701 agcacaacat gaaccagcag accacacaac aggaccagac cacacgtaaa ccatcacggc
   107761 gcataccgtg gcagcacatg acaaagcggc agcgaaaaaa ccgcaggagg ataaaccgac
   107821 tggctgagta ctggcctgaa ctgtttgggg cagagaatat aaaacccctt aaggctggcg
   107881 tgatacacga tatgttgcag gacgcgaagg cgcgggaact ggcgattggt catggcgtat
   107941 tacgtggcgc attaacctca caccttcaca cgacgcgata ccttaaggcg attattgctg
   108001 gtggcccacg gtacgacctg aacgggcaac cttgcggcga agtcacggag aaagagaaag
   108061 cagcagccag cgaacaactg atgtggaggc tggagcagat aaaacagcaa aacatccagc
   108121 ggcaggatac gggaggtggt gagagtgata acagacagcg tggcacagaa acttgaggag
   108181 cgcggtttat ggcgacgtgc ggcgacccga tgggctgagg ttcttcttca cgcagaaacg
   108241 gacagtgaac gcgaggaagc tgccagacgt cgggcgatat gcatcagtaa aacccgtcgt
   108301 atgcctgaac aactggtcac ctttggcgat gtgaaaaagg ccgcagacag aacgctgaaa
   108361 gagatgggga ttaatccgca ggatgaatgg aaaaattaca gcttcagtga tgcaggcgac
   108421 gatttagcgc tatcgtgata cgcatatcat cgccgtattt gctcaattat cgcgattcgc
   108481 tataatcacc accgaacggc cgttgatggt gttctccgca ggcaacggga gcctcgccga
   108541 atgaagccag ccagcggctt ttcggcactc attttgatgt gtacttgtca cacacatcgg
   108601 gatgagtgaa cgacgaaaac atcacggtga tttcgtcatg ccgcgataag aatgcgcctc
   108661 tttgggcaac tggagccctc tgtacagcgg gttattgcgg caaagttgtg ataaaaacgc
   108721 gcctctttga gcaattgaag ctcactgtaa agcggcttat cacagcaggc tgtaacgggc
   108781 gctcatcttc tggcaactga agccacctgt acagagaccc gttacggctg accctgaatg
   108841 atttcgctcc gtgcaactga agcacccagc ggagttattc aggttttttt attgcccgtc
   108901 caccagcacc acatctgtag cattgatgcg tatacattcg ccgtcaattg caccgttccg
   108961 gccatcaccg tcacgcacca caacagaatg accaggcagg ccataacgcg ccacctttcg
   109021 acgaacacca tcacctgttc cctcacgccg ctcatacacg ttaacccgct ccagaagacc
   109081 gtcacggacc atcgcttcaa gtgtgcgcct tgttgattcg agctgatatc ggttgttgtg
   109141 tgaatccatg ccatacagca ggtaagccac actggagaca tcaaacggcg ggttaccaat
   109201 ttcacctctg acccacgcca gattgtcggg agtgaagtaa ctgaggatct cttttttgta
   109261 ttgcgtcagt ctcatagtga cctttcttgt tgtaggatga atccctcata caataagcca
   109321 taaggcaaat tttgaaagga cagaatcggc ggggtacaga tgatacgtga tgacaaggct
   109381 gaagcgctgg agaatagcgg cctctggcgg cgtgctgctg aacgctgggg agaattgttc
   109441 agacaggcaa aaagtgatga agagaggcac tatattaccc aacgaagggc acaatgtacg
   109501 gcaaaagcca ccagaccaaa aacaacagag tgggggcatg tatgcgccgt cagggcagca
   109561 gcgaccagaa cgcttaaaga tatggggatt gaccagaaaa aagaagatcc tttacggggc
   109621 acgaagtaca ggggtgaaac agggcgaaga gacggaagtt aatatggaca caataactga
   109681 taaaaaagcc gaacaactgg aatctcaggg actatggcga agagccgccg ctcgttggct
   109741 tgacgtaatg aaagaggccc acaccgatcc gcagcgagag catatagcca gacggcggga
   109801 aatatgcctt gcgaacttca ggatgctgta aataagactg ttgacgatat gggaatcaga
   109861 cgggatcggc gacattacgg gttatggaaa taacagccag gtaaattggt gaatatacgg
   109921 caggctgcaa tcagagccga acatagcggc ctccgtcacc attatcagac agtagcggta
   109981 gtccttccat cacagcaaaa tgctacccac ttcacagccc cttagaagcc tcttcgatgg
   110041 ggtgaaaaat ccctgaaaaa atgcgcaaaa aactgcgcaa atcgatcata aaaccgatca
   110101 caaaatcgat cacacagacc agcagagcaa agccagatat gacgtggctt gtagggggaa
   110161 aagccaccgg aaaaagtgcg catttttctc aaaaacgaaa cgcaacagga agtgcaaaac
   110221 gaaaccgata cgcgatgatc gaaaaagcgc gcaaatttac cgggttcggg taagcaattt
   110281 tgcctgattt gggcaaaata cggtgtcgcc gtaacagtga cattgacacc aaaacagtga
   110341 cacacaggag ggccgatgac cgaacgttca ccaacagaga taattcacgg gattattgaa
   110401 gagcaggagg aacgcgagtt aatcctcatg attgaagagc agagagaacg ccagcgcaaa
   110461 caggaggcca cgcagccgga cagcgtgagc gctgaaaatc aggctgatgt gacgtgatgg
   110521 tgtcgaactg agtagcttta agcgactctg tgactggttt taaacgagtc gcaaaaagag
   110581 ccggagaaaa accacgaatc tggggttttt tctaagcacg ctgtacacgg tggaacatat
   110641 ggtggtcagg agacggcagg agcaacgcca gaacgcgccc ccgtaccagt ggcataagcc
   110701 ggagcggtta cggcggtaac ttgttgatta aaaggcggct caattctgag ccgggtagct
   110761 gacggcgtta actggctgat attgttacaa agctcatttt tgagctttgg cctaacctac
   110821 tgatattttc ggaaacctca atttgaggag accgcaaacg caaaattgcg ctggctacat
   110881 tcctcacttc tgaggactgt taacaagctg gctgaaagac agtcacaagc tacgaaattt
   110941 tcgtagttta actttcatcc agtgcatccc tgaattacgg gtatgtaacc acagctatta
   111001 cgaggtgatt gttgatgcgt gatattcagc aggttctgga acgctgggga gcgtgggcgg
   111061 caaataatcg cgagaatgtg acctggtcat ccgttgccgc aggatttaag gggattattc
   111121 cggcaaagat aacatcacgc tcacagtgct gtgacgatga tgcgatggtg atatgcgggt
   111181 gcatagcccg tctttaccgg aacaatcgcg atctgcatga cttgctggtt gattattacg
   111241 tgttggggga gacgttcatg gcgctggcac ggaaacatgg gtgctctgac acctgtatag
   111301 gtaaacgcct tcacaaagcg gagggggttg ttgaaggcat gttgatgatg ctggatgtga
   111361 agctggagat ggatcggtat gttgagcgcg attcagtgac ggcataacgg cataaaaaaa
   111421 ccgcagtcat gaggaaactg cggtggagaa atacggcttg ccaatcaatc atgaaaaacg
   111481 taatcgcctg ataattatgc cacgagacaa aggacggtgc accctgaatg gtcataatga
   111541 ccactcagac catcgaggca accataacga acatcgttac agttggagct tttaccccgt
   111601 tgggggaaaa ggtgcatacc tgaatcatca ttatggtgat caagggcatg taaagcccac
   111661 cagcctgatt aaaggttaac cggaaaaagc caggtatgga gggagtgatt agccacgaac
   111721 aagccggaca ccaccagcag aaggtattat gattttcgta atacctttag caaagcgtga
   111781 atcatcatta ccgtagggat ggtcataatg accacacctt cccccaccgg aacaaggaag
   111841 gctggcttac gaaaatttcg taaccctccc cagcacaatg tattaaataa tcactacagg
   111901 ccattgatta aacttaatta tcgcgaatcg cgagaattag ccccgccgga acaaggaaag
   111961 ggaaattacg aaaatttcgt aaaatccccc cacatctccc acggttgccc gttaagtgca
   112021 agcgcacagg cacgacctca ttaaccagtt accagcgctc acgctcaaac atccacagca
   112081 atggggataa atcgcactct ggcgcaatgt tcactatcgg ggcgagcatt caccggaaaa
   112141 cgtcatacag gcttacaggc ggcttaaaat tcgatgggcg gaggaccgga tgggaatcgg
   112201 gagacctgga caaaaagtgc aggcctccat gacacggtca ggacagtttg tgtttaacca
   112261 gaatatttag cgcctgtatt ggggcttcgt ggagctcccg cagacattca gggtcagtaa
   112321 catgttcctg gtatcttgtt ttctcaagta acaagcggtg catatgcagc atacgggcaa
   112381 gagccgcgca cacctcctca aatgcggtat cccgcttacc gtcactgtca gagccacaaa
   112441 acagcggtac aacctccacg ccgtcgctgt gggcctttag ccaggcatca accacggtta
   112501 aatcatcggt gaagaaccac gcactgttag cgctgaatcg cattttccag gcttccggta
   112561 ctggttgcgg ggagtatgaa tatgctggtg gtatcttgtc gtgagtatcc ataggctatc
   112621 gttccgtcat tctgttattc accacagcgc taaaaacgcc atgaaggacc agaataccga
   112681 ttgcctcgtt tgctgtatga ggtgcttacc acacattggg tacacagatg aatcaggcgg
   112741 aagtgtgatt accggaaaaa acccctttca gtttgacgaa aaaaaaaccg cagtgtgata
   112801 acactacggc acgccaccaa agaaacataa caaggaatca gcaatgagta atacctgtaa
   112861 gccttcgggg tgattgtaac acatgagcaa tcttttactg gaagctgttt ttcactggat
   112921 cacgtatcgt tatggggatt cccatatcgg agagcatcca ccagcttcat agcggataac
   112981 cggaaaaatt ccggtaaata tttgcctgag taagtcatca agcgcctgct aaatgtgtat
   113041 agaaatgtgt ataggattga ttttagaata tgtgggaatg gctttattgc tggtggcatg
   113101 gcttgttgtt ctgttccctt cgccgctcca atttatcttc acatcatcta cctgccattt
   113161 ttgttgtatc tgattttcta tattatctta attcaagcgc ttcagacttt tcgcaatcgc
   113221 cgtaatactt ccccattttt ttgtaaataa aaagcgttta cgcaatgcaa tcgtcatgta
   113281 tcttaaatag tcttatttaa gatgtttaga act
//
LOCUS       DAZGTJ010000004       107468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000004 DAZGTJ010000000
VERSION     DAZGTJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 107468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.004"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            207..542
                     /gene="yjdM"
                     /locus_tag="ACHK8E_000369"
     CDS             207..542
                     /gene="yjdM"
                     /locus_tag="ACHK8E_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP0342690.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            702..1145
                     /gene="yjdN"
                     /locus_tag="ACHK8E_000370"
     CDS             702..1145
                     /gene="yjdN"
                     /locus_tag="ACHK8E_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP0342691.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1278..2066
                     /gene="phnC"
                     /locus_tag="ACHK8E_000371"
     CDS             1278..2066
                     /gene="phnC"
                     /locus_tag="ACHK8E_000371"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP0342692.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2091..3107
                     /gene="phnD"
                     /locus_tag="ACHK8E_000372"
     CDS             2091..3107
                     /gene="phnD"
                     /locus_tag="ACHK8E_000372"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0342693.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3213..3992
                     /gene="phnE"
                     /locus_tag="ACHK8E_000373"
     CDS             3213..3992
                     /gene="phnE"
                     /locus_tag="ACHK8E_000373"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP0342694.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4013..4738
                     /gene="phnF"
                     /locus_tag="ACHK8E_000374"
     CDS             4013..4738
                     /gene="phnF"
                     /locus_tag="ACHK8E_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP0342695.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4739..5191
                     /gene="phnG"
                     /locus_tag="ACHK8E_000375"
     CDS             4739..5191
                     /gene="phnG"
                     /locus_tag="ACHK8E_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP0342696.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5188..5772
                     /gene="phnH"
                     /locus_tag="ACHK8E_000376"
     CDS             5188..5772
                     /gene="phnH"
                     /locus_tag="ACHK8E_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP0342697.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5772..6836
                     /gene="phnI"
                     /locus_tag="ACHK8E_000377"
     CDS             5772..6836
                     /gene="phnI"
                     /locus_tag="ACHK8E_000377"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP0342698.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6829..7674
                     /gene="phnJ"
                     /locus_tag="ACHK8E_000378"
     CDS             6829..7674
                     /gene="phnJ"
                     /locus_tag="ACHK8E_000378"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP0342699.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7671..8429
                     /gene="phnK"
                     /locus_tag="ACHK8E_000379"
     CDS             7671..8429
                     /gene="phnK"
                     /locus_tag="ACHK8E_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP0342700.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8540..9220
                     /gene="phnL"
                     /locus_tag="ACHK8E_000380"
     CDS             8540..9220
                     /gene="phnL"
                     /locus_tag="ACHK8E_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP0342701.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9217..10353
                     /gene="phnM"
                     /locus_tag="ACHK8E_000381"
     CDS             9217..10353
                     /gene="phnM"
                     /locus_tag="ACHK8E_000381"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP0342702.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10353..10910
                     /gene="phnN"
                     /locus_tag="ACHK8E_000382"
     CDS             10353..10910
                     /gene="phnN"
                     /locus_tag="ACHK8E_000382"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP0342703.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10897..11331
                     /gene="phnO"
                     /locus_tag="ACHK8E_000383"
     CDS             10897..11331
                     /gene="phnO"
                     /locus_tag="ACHK8E_000383"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP0342704.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11333..12091
                     /gene="phnP"
                     /locus_tag="ACHK8E_000384"
     CDS             11333..12091
                     /gene="phnP"
                     /locus_tag="ACHK8E_000384"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP0342705.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(12119..12448)
                     /gene="yjdP"
                     /locus_tag="ACHK8E_000385"
     CDS             complement(12119..12448)
                     /gene="yjdP"
                     /locus_tag="ACHK8E_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP0342706.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12517..12966)
                     /gene="rpiB"
                     /locus_tag="ACHK8E_000386"
                     /gene_synonym="alsI"
     CDS             complement(12517..12966)
                     /gene="rpiB"
                     /locus_tag="ACHK8E_000386"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFP0342707.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13325..14215
                     /gene="alsR"
                     /locus_tag="ACHK8E_000387"
     CDS             13325..14215
                     /gene="alsR"
                     /locus_tag="ACHK8E_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFP0342708.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14274..15209
                     /gene="alsB"
                     /locus_tag="ACHK8E_000388"
     CDS             14274..15209
                     /gene="alsB"
                     /locus_tag="ACHK8E_000388"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFP0342709.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGETRRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15337..16494
                     /gene="alsA"
                     /locus_tag="ACHK8E_000389"
                     /pseudo
     CDS             15337..16494
                     /gene="alsA"
                     /locus_tag="ACHK8E_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
     gene            16483..17256
                     /gene="alsK"
                     /locus_tag="ACHK8E_000390"
                     /pseudo
     CDS             16483..17256
                     /gene="alsK"
                     /locus_tag="ACHK8E_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
     gene            17529..19514
                     /gene="yjcS"
                     /locus_tag="ACHK8E_000391"
     CDS             17529..19514
                     /gene="yjcS"
                     /locus_tag="ACHK8E_000391"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP0342710.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            19723..19998
                     /gene="ytcA"
                     /locus_tag="ACHK8E_000392"
     CDS             19723..19998
                     /gene="ytcA"
                     /locus_tag="ACHK8E_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP0342711.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            20017..21048
                     /gene="mdtN"
                     /locus_tag="ACHK8E_000393"
     CDS             20017..21048
                     /gene="mdtN"
                     /locus_tag="ACHK8E_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP0342712.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            21048..23099
                     /gene="mdtO"
                     /locus_tag="ACHK8E_000394"
     CDS             21048..23099
                     /gene="mdtO"
                     /locus_tag="ACHK8E_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0342713.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            23096..24562
                     /gene="mdtP"
                     /locus_tag="ACHK8E_000395"
     CDS             23096..24562
                     /gene="mdtP"
                     /locus_tag="ACHK8E_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP0342714.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            24760..26907
                     /gene="fdhF"
                     /locus_tag="ACHK8E_000396"
     CDS             24760..26907
                     /gene="fdhF"
                     /locus_tag="ACHK8E_000396"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:25177..25179,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP0342715.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            27001..27690
                     /gene="yjcO"
                     /locus_tag="ACHK8E_000397"
     CDS             27001..27690
                     /gene="yjcO"
                     /locus_tag="ACHK8E_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP0342716.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(28108..29421)
                     /gene="gltP"
                     /locus_tag="ACHK8E_000398"
     CDS             complement(28108..29421)
                     /gene="gltP"
                     /locus_tag="ACHK8E_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP0342717.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(29763..30359)
                     /gene="nrfG"
                     /locus_tag="ACHK8E_000399"
     CDS             complement(29763..30359)
                     /gene="nrfG"
                     /locus_tag="ACHK8E_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP0342718.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(30356..30739)
                     /gene="nrfF"
                     /locus_tag="ACHK8E_000400"
     CDS             complement(30356..30739)
                     /gene="nrfF"
                     /locus_tag="ACHK8E_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP0342719.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(30732..32450)
                     /gene="nrfE"
                     /locus_tag="ACHK8E_000401"
     CDS             complement(30732..32450)
                     /gene="nrfE"
                     /locus_tag="ACHK8E_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP0342720.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(32470..33426)
                     /gene="nrfD"
                     /locus_tag="ACHK8E_000402"
     CDS             complement(32470..33426)
                     /gene="nrfD"
                     /locus_tag="ACHK8E_000402"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP0342721.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(33423..34094)
                     /gene="nrfC"
                     /locus_tag="ACHK8E_000403"
     CDS             complement(33423..34094)
                     /gene="nrfC"
                     /locus_tag="ACHK8E_000403"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP0342722.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(34091..34657)
                     /gene="nrfB"
                     /locus_tag="ACHK8E_000404"
     CDS             complement(34091..34657)
                     /gene="nrfB"
                     /locus_tag="ACHK8E_000404"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP0342723.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(34702..36138)
                     /gene="nrfA"
                     /locus_tag="ACHK8E_000405"
     CDS             complement(34702..36138)
                     /gene="nrfA"
                     /locus_tag="ACHK8E_000405"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP0342724.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            36531..38489
                     /gene="acs"
                     /locus_tag="ACHK8E_000406"
     CDS             36531..38489
                     /gene="acs"
                     /locus_tag="ACHK8E_000406"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP0342725.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            38871..39185
                     /gene="yjcH"
                     /locus_tag="ACHK8E_000407"
     CDS             38871..39185
                     /gene="yjcH"
                     /locus_tag="ACHK8E_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP0342726.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            39182..40831
                     /gene="actP"
                     /locus_tag="ACHK8E_000408"
     CDS             39182..40831
                     /gene="actP"
                     /locus_tag="ACHK8E_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP0342727.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(40914..42563)
                     /gene="yjcE"
                     /locus_tag="ACHK8E_000409"
     CDS             complement(40914..42563)
                     /gene="yjcE"
                     /locus_tag="ACHK8E_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP0342728.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(42714..44063)
                     /gene="ghxP"
                     /locus_tag="ACHK8E_000410"
                     /gene_synonym="yjcD"
     CDS             complement(42714..44063)
                     /gene="ghxP"
                     /locus_tag="ACHK8E_000410"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP0342729.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(44609..45073)
                     /gene="soxR"
                     /locus_tag="ACHK8E_000411"
     CDS             complement(44609..45073)
                     /gene="soxR"
                     /locus_tag="ACHK8E_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP0342730.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            45159..45482
                     /gene="soxS"
                     /locus_tag="ACHK8E_000412"
     CDS             45159..45482
                     /gene="soxS"
                     /locus_tag="ACHK8E_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP0342731.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(45485..47071)
                     /gene="pdeC"
                     /locus_tag="ACHK8E_000413"
     CDS             complement(45485..47071)
                     /gene="pdeC"
                     /locus_tag="ACHK8E_000413"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP0342732.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            47501..47782
                     /gene="yjcB"
                     /locus_tag="ACHK8E_000414"
     CDS             47501..47782
                     /gene="yjcB"
                     /locus_tag="ACHK8E_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP0342733.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(47882..48418)
                     /gene="ssb1"
                     /locus_tag="ACHK8E_000415"
     CDS             complement(47882..48418)
                     /gene="ssb1"
                     /locus_tag="ACHK8E_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP0342734.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            48673..51495
                     /gene="uvrA"
                     /locus_tag="ACHK8E_000416"
     CDS             48673..51495
                     /gene="uvrA"
                     /locus_tag="ACHK8E_000416"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP0342735.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(51530..51886)
                     /gene="yjbR"
                     /locus_tag="ACHK8E_000417"
     CDS             complement(51530..51886)
                     /gene="yjbR"
                     /locus_tag="ACHK8E_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0342736.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(51890..52306)
                     /gene="yjbQ"
                     /locus_tag="ACHK8E_000418"
     CDS             complement(51890..52306)
                     /gene="yjbQ"
                     /locus_tag="ACHK8E_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP0342737.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(52417..53130)
                     /gene="aphA"
                     /locus_tag="ACHK8E_000419"
     CDS             complement(52417..53130)
                     /gene="aphA"
                     /locus_tag="ACHK8E_000419"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP0342738.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            53531..53753
                     /locus_tag="ACHK8E_000420"
                     /pseudo
     CDS             53531..53753
                     /locus_tag="ACHK8E_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54256..55449)
                     /gene="tyrB"
                     /locus_tag="ACHK8E_000421"
     CDS             complement(54256..55449)
                     /gene="tyrB"
                     /locus_tag="ACHK8E_000421"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP0342739.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(55702..56781)
                     /gene="alr"
                     /locus_tag="ACHK8E_000422"
     CDS             complement(55702..56781)
                     /gene="alr"
                     /locus_tag="ACHK8E_000422"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP0342740.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(56834..58249)
                     /gene="dnaB"
                     /locus_tag="ACHK8E_000423"
     CDS             complement(56834..58249)
                     /gene="dnaB"
                     /locus_tag="ACHK8E_000423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0342741.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            58332..59315
                     /gene="qorA"
                     /locus_tag="ACHK8E_000424"
     CDS             58332..59315
                     /gene="qorA"
                     /locus_tag="ACHK8E_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP0342742.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(59481..59723)
                     /gene="pspG"
                     /locus_tag="ACHK8E_000425"
     CDS             complement(59481..59723)
                     /gene="pspG"
                     /locus_tag="ACHK8E_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP0342743.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(59857..60852)
                     /gene="dusA"
                     /locus_tag="ACHK8E_000426"
     CDS             complement(59857..60852)
                     /gene="dusA"
                     /locus_tag="ACHK8E_000426"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP0342744.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            61008..61523
                     /gene="zur"
                     /locus_tag="ACHK8E_000427"
     CDS             61008..61523
                     /gene="zur"
                     /locus_tag="ACHK8E_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP0342745.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(61565..61774)
                     /gene="yjbJ"
                     /locus_tag="ACHK8E_000428"
     CDS             complement(61565..61774)
                     /gene="yjbJ"
                     /locus_tag="ACHK8E_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP0342746.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(61890..63215)
                     /gene="dinF"
                     /locus_tag="ACHK8E_000429"
     CDS             complement(61890..63215)
                     /gene="dinF"
                     /locus_tag="ACHK8E_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP0342747.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(63288..63896)
                     /gene="lexA"
                     /locus_tag="ACHK8E_000430"
     CDS             complement(63288..63896)
                     /gene="lexA"
                     /locus_tag="ACHK8E_000430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP0342748.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(64006..64374)
                     /gene="dgkA"
                     /locus_tag="ACHK8E_000431"
     CDS             complement(64006..64374)
                     /gene="dgkA"
                     /locus_tag="ACHK8E_000431"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0342749.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            64545..66968
                     /gene="plsB"
                     /locus_tag="ACHK8E_000432"
     CDS             64545..66968
                     /gene="plsB"
                     /locus_tag="ACHK8E_000432"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP0342750.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(67123..67995)
                     /gene="ubiA"
                     /locus_tag="ACHK8E_000433"
     CDS             complement(67123..67995)
                     /gene="ubiA"
                     /locus_tag="ACHK8E_000433"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP0342751.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(68008..68505)
                     /gene="ubiC"
                     /locus_tag="ACHK8E_000434"
     CDS             complement(68008..68505)
                     /gene="ubiC"
                     /locus_tag="ACHK8E_000434"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP0342752.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(68684..69604)
                     /gene="malM"
                     /locus_tag="ACHK8E_000435"
     CDS             complement(68684..69604)
                     /gene="malM"
                     /locus_tag="ACHK8E_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP0342753.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(69842..71182)
                     /gene="lamB"
                     /locus_tag="ACHK8E_000436"
     CDS             complement(69842..71182)
                     /gene="lamB"
                     /locus_tag="ACHK8E_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP0342754.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(71254..72369)
                     /gene="malK"
                     /locus_tag="ACHK8E_000437"
     CDS             complement(71254..72369)
                     /gene="malK"
                     /locus_tag="ACHK8E_000437"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP0342755.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            72734..73924
                     /gene="malE"
                     /locus_tag="ACHK8E_000438"
     CDS             72734..73924
                     /gene="malE"
                     /locus_tag="ACHK8E_000438"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP0342756.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            74078..75622
                     /gene="malF"
                     /locus_tag="ACHK8E_000439"
     CDS             74078..75622
                     /gene="malF"
                     /locus_tag="ACHK8E_000439"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP0342757.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            75637..76527
                     /gene="malG"
                     /locus_tag="ACHK8E_000440"
     CDS             75637..76527
                     /gene="malG"
                     /locus_tag="ACHK8E_000440"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP0342758.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(76621..77031)
                     /gene="psiE"
                     /locus_tag="ACHK8E_000441"
     CDS             complement(76621..77031)
                     /gene="psiE"
                     /locus_tag="ACHK8E_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP0342759.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            77245..77523
                     /locus_tag="ACHK8E_000442"
     CDS             77245..77523
                     /locus_tag="ACHK8E_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342760.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(77570..79666)
                     /gene="yjbH"
                     /locus_tag="ACHK8E_000443"
     CDS             complement(77570..79666)
                     /gene="yjbH"
                     /locus_tag="ACHK8E_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0342761.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(79666..80403)
                     /gene="yjbG"
                     /locus_tag="ACHK8E_000444"
     CDS             complement(79666..80403)
                     /gene="yjbG"
                     /locus_tag="ACHK8E_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0342762.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(80400..81038)
                     /gene="yjbF"
                     /locus_tag="ACHK8E_000445"
     CDS             complement(80400..81038)
                     /gene="yjbF"
                     /locus_tag="ACHK8E_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP0342763.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(81225..81467)
                     /gene="yjbE"
                     /locus_tag="ACHK8E_000446"
     CDS             complement(81225..81467)
                     /gene="yjbE"
                     /locus_tag="ACHK8E_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP0342764.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            complement(81965..83614)
                     /gene="pgi"
                     /locus_tag="ACHK8E_000447"
     CDS             complement(81965..83614)
                     /gene="pgi"
                     /locus_tag="ACHK8E_000447"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP0342765.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      83851..84047
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            84139..85488
                     /gene="lysC"
                     /locus_tag="ACHK8E_000448"
     CDS             84139..85488
                     /gene="lysC"
                     /locus_tag="ACHK8E_000448"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP0342766.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            85741..86013
                     /gene="yjbD"
                     /locus_tag="ACHK8E_000449"
     CDS             85741..86013
                     /gene="yjbD"
                     /locus_tag="ACHK8E_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP0342767.1"
                     /translation="MALPRITQKEMNEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(86014..86886)
                     /gene="rluF"
                     /locus_tag="ACHK8E_000450"
     CDS             complement(86014..86886)
                     /gene="rluF"
                     /locus_tag="ACHK8E_000450"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP0342768.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            87234..88181
                     /locus_tag="ACHK8E_000451"
     CDS             87234..88181
                     /locus_tag="ACHK8E_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP0342769.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            88251..89057
                     /locus_tag="ACHK8E_000452"
     CDS             88251..89057
                     /locus_tag="ACHK8E_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0342770.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            89067..89474
                     /locus_tag="ACHK8E_000453"
     CDS             89067..89474
                     /locus_tag="ACHK8E_000453"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP0342771.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            89474..89968
                     /locus_tag="ACHK8E_000454"
     CDS             89474..89968
                     /locus_tag="ACHK8E_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP0342772.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            90034..90831
                     /locus_tag="ACHK8E_000455"
     CDS             90034..90831
                     /locus_tag="ACHK8E_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP0342773.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            90842..91666
                     /locus_tag="ACHK8E_000456"
     CDS             90842..91666
                     /locus_tag="ACHK8E_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP0342774.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            91718..92947
                     /gene="sorE"
                     /locus_tag="ACHK8E_000457"
     CDS             91718..92947
                     /gene="sorE"
                     /locus_tag="ACHK8E_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP0342775.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            93329..94018
                     /gene="pepE"
                     /locus_tag="ACHK8E_000458"
     CDS             93329..94018
                     /gene="pepE"
                     /locus_tag="ACHK8E_000458"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP0342776.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(94108..95739)
                     /gene="yjbB"
                     /locus_tag="ACHK8E_000459"
     CDS             complement(94108..95739)
                     /gene="yjbB"
                     /locus_tag="ACHK8E_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP0342777.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGICLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(95959..99642)
                     /gene="metH"
                     /locus_tag="ACHK8E_000460"
     CDS             complement(95959..99642)
                     /gene="metH"
                     /locus_tag="ACHK8E_000460"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP0342778.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            99842..100666
                     /gene="iclR"
                     /locus_tag="ACHK8E_000461"
     CDS             99842..100666
                     /gene="iclR"
                     /locus_tag="ACHK8E_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP0342779.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(100683..102407)
                     /gene="aceK"
                     /locus_tag="ACHK8E_000462"
     CDS             complement(100683..102407)
                     /gene="aceK"
                     /locus_tag="ACHK8E_000462"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP0342780.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(102623..103927)
                     /gene="aceA"
                     /locus_tag="ACHK8E_000463"
     CDS             complement(102623..103927)
                     /gene="aceA"
                     /locus_tag="ACHK8E_000463"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP0342781.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(103957..105558)
                     /gene="aceB"
                     /locus_tag="ACHK8E_000464"
     CDS             complement(103957..105558)
                     /gene="aceB"
                     /locus_tag="ACHK8E_000464"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP0342782.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(105827..106756)
                     /gene="metA"
                     /locus_tag="ACHK8E_000465"
     CDS             complement(105827..106756)
                     /gene="metA"
                     /locus_tag="ACHK8E_000465"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP0342783.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            106913..107356
                     /gene="yjaB"
                     /locus_tag="ACHK8E_000466"
     CDS             106913..107356
                     /gene="yjaB"
                     /locus_tag="ACHK8E_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0342784.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT        25982 a        28909 c        27871 g        24706 t
ORIGIN      
        1 cgagtagagc attaacctta tcttaaataa taaatggagg caaaaagatt attttctttt
       61 tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact
      121 cagggatttt gtgtataatt gcggcctttt tcggcaatct gccgtttttt ggcgcttttg
      181 ccctgctgac ttttgaggaa atccacatgt cattaccaca ctgcccaaaa tgcaactccg
      241 aatacaccta cgaagataac ggcatgtaca tctgcccgga atgtgcctac gaatggaacg
      301 acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa agatgctaac ggcaatctgc
      361 tggctgacgg cgacagcgtt accatcatta aagatctgaa agtgaaaggt agctcttcga
      421 tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg
      481 attgcaaaat cgacggtttt ggtccgatga aactgaagtc tgagtttgtg aaaaagaact
      541 gattgtattg tgatcggtag gccggataag gcgtttacgc cgcatccggc aacggtggcg
      601 actgcctgat gcgacgctga ccgcgtctta tcaggcttcc ctctcatctg tataaatttc
      661 gaactacact taacgggctt ctcttaactg aggtcaccat catgccgtta agtccctacc
      721 tctcttttgc cggtaactgt tccgacgcga ttgcctatta tcaacgtacg ttgggcgcgg
      781 aactgctcta taaaatcagc ttcggcgaaa tgccgaaatc agcgcaggac agcgccgaga
      841 actgcccttc cggaatgcaa tttcccgata ccgccatcgc tcatgccaac gtgcgcattg
      901 caggaagcga catcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggct
      961 ttacgctagt gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg
     1021 cagctaacgg aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggcttcggca
     1081 aagtcaccga taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc
     1141 actaacccgc cgggagggcc gccctcccgc actgtcatcg aaatctcgtt aactcttcat
     1201 ccgttagtca cttttagtta acaaaatcgt cacaataatc cgccacgatg gagccacttt
     1261 tttagggagg ctgcatcatg caaacgatta tccgtgtcga gaagctcgcc aaaaccttca
     1321 atcagcatca ggcgctgcac gcggttgatc tgaacattca tcacggtgaa atggtggctc
     1381 tgcttgggcc gtcgggttcc ggcaaatcca cccttttacg tcacttaagc ggtttgatta
     1441 ccggcgataa atctgccggt agccatatcg agctgctggg ccgcacagtc cagcgcgaag
     1501 gccgcctggc ccgcgatatc cgcaaaagcc gcgccaatac cggctacatc ttccaacaat
     1561 tcaacctggt gaatcgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca
     1621 cgccattctg gcgcacctgt tttagctact tcacccgcga gcagaaacag cgcgcgttac
     1681 aggcgctgac ccgcgttggc atggtgcact ttgcccatca gcgcgtttcc accctctccg
     1741 gcggacagca acagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg aaggtgattc
     1801 tggcggacga acccatcgcc tcgctggacc cggaatcggc gcgcatcgtg atggacaccc
     1861 tgcgcgacat taaccagaac gacggcatta ccgtggtcgt cacgctgcat caggtggatt
     1921 acgccctgcg ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtc ttctacgacg
     1981 gcagcagcca acagtttgat aacgaacgtt ttgaccatct ctaccgcagc atcaaccgcg
     2041 tcgaagaaaa cgcgaaagct gcctgacatc cccatcattg aggaaaacga atgaacgcta
     2101 agataattgc ctcgctgacc ttcaccagca tgttcagcct cagcaccctg ttaagcccgg
     2161 cgcacgccga agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc
     2221 aaaacctgaa accgcaatgg acgccgttct tacaggatat ggagaagaag ctgggcgtga
     2281 aggtcaacgc cttctttgcc ccggactacg cgggcattat ccagggaatg cgcttcaaca
     2341 aagtggatat cgcctggtac ggcaatctgt cagcgatgga agcggtggat cgcgccaatg
     2401 gccaggtctt cgcccagacg gtcgcggcgg atggatcgcc gggttactgg agcgtgttga
     2461 tcgtcaacaa agatagtccg atcaacaacc tgaacgatct gctggccaag cgtaaagatc
     2521 tcaccttcgg caatggcgat cctaactcca cctcaggctt cctcgtcccc ggctactacg
     2581 tcttcgccaa aaacaatatc tccgccagcg acttcaagcg caccgtcaac gccgggcatg
     2641 aaaccaacgc gctggccgtt gccaacaagc aggtggatgt tgccaccaac aacaccgaaa
     2701 acctcgacaa gctgaaaacc tccgcaccgg agaagctcaa agaactgaag gtgatctgga
     2761 aatcgccgct gatcccaggc gatccgatcg tctggcgcaa aaatctgtcc gaaaccacca
     2821 aagacaagat ctacgacttc tttatgaact acggcaaaac gccggaagag aaagcggtgc
     2881 tggaacgcct gggctgggcc ccgttccgcg cctccagcga cctgcaactg gtgccgattc
     2941 gccagctcgc gctgtttaaa gagatgcagg gcgtgaaagg caataaaggg ctgaatgagc
     3001 aggacaagct ggcaaaaacc agcgccattc aggcgcaact ggatgacctg gaccgcctga
     3061 acaacgcgtt aagcgcgatg agttcggtga gtaaagcggt gcagtaaatc gtaggtcgga
     3121 taagacgccc cggcgtcgca tccgacaatg tgcagacgtt gatgccggat gcggtgcaag
     3181 caccttatcc ggcctacaga ccggagccaa acatgcaaac catcaccatc gccccaccca
     3241 agcgcagctg gttctcgctt ctgagctggg ccattgttct cgccgtgttg gtcgtctcgt
     3301 ggcagggcgc ggaaatggcc ccgcttacgc tgatcaaaga cggcggcaac atggcaacct
     3361 tcgccgccga cttcttcccg cccgatttca gccagtggca ggattacctc accgaaatgg
     3421 ccgtcacgct gcaaatcgcc gtctggggca ccgcgctggc ggtggttctc tccatcccct
     3481 tcggtctgat gagcgccgaa aacctggtgc cgtggtgggt ttaccagccc gtacgccgcc
     3541 tgatggacgc ctgccgcgcc attaacgaaa tggtcttcgc catgctgttc gtggtcgccg
     3601 tcggccttgg cccgttcgct ggcgtgctgg cgctgtttat ccacaccacc ggcgtgctct
     3661 ccaagctgct ttccgaagcg gtagaagcga tcgaacccgg cccggtggag ggcattcgcg
     3721 ccaccggtgc caacaagctg gaagagatcc tctacggcgt gctgccacag gtgatgccgc
     3781 tgctgatctc ctactccctc tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg
     3841 gcatggtcgg tgcaggcggg atcggcgtca ccctgtggga agcgattcgc ggtttccagt
     3901 tccagcaaac ctgcgccctg atggtgctca tcatcgtcac ggtcagcctg ctggatttcc
     3961 tctctcaacg gttgcgtaag cactttatct gataagcgag gcattgatag ctatgcactt
     4021 gtctacacat ccgaccagct acccaacacg ctatcaggag atagccgcaa aacttgagca
     4081 ggagcttcgt caacactacc gctgcggcga ctatcttccc gccgagcagc aactggcagc
     4141 gcgctttgag gtgaaccgcc acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg
     4201 ttgggtacag cgccgtcagg gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc
     4261 gctcaacgcc caggcgcgtt ttagccagaa cctgctggac caaggcagcc atcccaccag
     4321 cgaaaaattg ctatcggtgc tgcgccccgc ctccggtcac gtcgctgacg cgctggggat
     4381 aaccgagggg gagaacgtca tccacctgcg caccctgcgc cgggtcaacg gcgtcgcgct
     4441 ctgtttaatc gaccactact ttgcggacct caccctctgg ccgacgctgc aacatttcga
     4501 cagcggttcg ctgcacgatt ttctgcgcga gcaaacgggg attacgctac gccgcagtca
     4561 gacgcggatc agcgcccgcc gcgcccaggc caaagagtgc cagcgtcttg aaatcccgaa
     4621 tctatcgccg ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc
     4681 ggagtactcc gtcagcctga cgcgcgccga catgattgaa ttcactatgg agcactgaat
     4741 gcacgcagat accgcgaccc gccagcactg gatgtccgta ctggcgcaca gccaaccgac
     4801 tgagctggca gcccgcctga acgcgctgaa catcaccgcc gactatgaag taatccgcgc
     4861 cgctgaaacc ggcctggtgc agattcaggc gcggatgggt ggtaccggcg aacgcttttt
     4921 tgctggcgac gccacgctca cccgcgccgc cgtacgcctg actgacggca cgctcggcta
     4981 cagctgggtg ctggggcgcg acaaacagca cgccgaacgc tgcgcgctga ttgacgcgct
     5041 gatgcagcaa acccgccatt ttcaaaactt atcagaaact cttattgccc cgctgaacgc
     5101 tgaccgtatg gcacgcattg ccgcacgcca ggccgaagta aacgccagcc gggtcgactt
     5161 ctttacgatg gttcgcggag ataacgcatg accctggaaa ccgcttttat gcttcccgtg
     5221 caggatgccc agcacagttt tcgtcgcctg ttaaaagcca tgagcgagcc gggcgtgatt
     5281 gtcgccctgc atcagctcaa acgcggctgg cagccgctga atatcgccac caccagcgtg
     5341 ctgctgacgc tggccgataa cgacacgccg gtgtggcttt ctgcgccatt aagtaacgat
     5401 atcgtcagcc agagcctgcg ttttcatacc aacgcgccgc tggtcaatca gccggaactg
     5461 gcgaccttcg cggtaacgga tgaggcgatt tccagcgaac agcttaacgc cctttccagc
     5521 ggcaccgctg ttgcgccaga agcgggcgcg acgctgattt tacaggtcgt cagcctgagc
     5581 ggcgggcgca tgctgcgcct tactggcgcg ggtattgccg aagaacgaat gatcgccccg
     5641 cagctgccgg agtgcattct gcacgaactt accgagcgcc cgcacccatt cccgctcggc
     5701 atcgacctga tcctgacctg cggcgagcgc ctgctggcta tcccgcgaac cactcatgtg
     5761 gaggtatgct gatgtacgtt gccgtgaaag ggggcgagaa ggcgatcgac gccgcccacg
     5821 ccctgcaaga gagccgacgc cggggcaata ccgatttgcc tgaactgagc gtcgcccaga
     5881 ttgaacagca gcttaatctt gcagtagatc gcgtgatgac cgaaggcggc attgccgacc
     5941 gcgaactggc ggcgctggcg ctgaaacagg ccagcggcga taacgtcgaa gcgattttcc
     6001 tgctacgcgc ctaccgcacc acgctggcga agctcgcagt aagcgagccg ctcgacacca
     6061 cggagatgcg tctcgaacgg cgtatttcag cggtttataa agatattccc ggcggccagc
     6121 tgcttggccc aacctacgac tacacccatc gcctgctcga tttcaccctg ctggcaaacg
     6181 gcgaagcgcc gacgctgacc acagccgaca gcgaacaaca gccgtcgccg cacgttttca
     6241 gcctgctggc gcgtcagggg ctggcgaagt ttgaagaaga tagcggcgca cagccggacg
     6301 acatcacccg cacgccgccg gtttacccct gctcacgctc ctcccgcctg caacaattaa
     6361 tgcgcggcga cgaaggctat ttgctggcgc tggcctactc cacccagcgt ggttacggac
     6421 gcaatcatcc gttcgcaggc gaaatccgca gcggctatat cgacgtgtcg attgtgcagg
     6481 aagagctggg atttgcggtg aacgtcggcg aactgctgat gaccgaatgt gaaatggtca
     6541 acggttttat cgacccgccg gatgagccgc cgcacttcac gcgcggctac gggctggtgt
     6601 tcggcatgag cgagcgcaaa gcgatggcga tggcgctggt tgaccgcgct ctacaagccc
     6661 cggagtacgg cgagcacgcg acaggcccgg cgcaggatga agagttcgtg ctggcgcatg
     6721 ccgacaacgt cgaagccgca ggctttgtct cgcaccttaa actcccgcac tacgtcgatt
     6781 tccaggccga actggagcta ctcaaacgtc tgcaacagga gcagaaccat ggctaatctg
     6841 agcggctaca actttgccta cctcgacgag cagaccaaac gcatgatccg ccgcgccatc
     6901 ttaaaagcgg tggcgatccc cggttatcag gtgccgtttg gcgggcgcga gatgccgatg
     6961 ccgtacggct ggggaaccgg cggcatacag cttaccgcca gcgtgattgg cgaaagcgat
     7021 gtgctgaagg tgattgacca gggcgcggac gacaccacca acgcggtgtc gatccgtaac
     7081 ttctttaagc gcgtgaccgg ggtaaacacc actgaacgta cggacgatgc gacgcttatc
     7141 cagacgcgcc accgcatccc cgaaacgccg ctgaccgaag atcagatcat tattttccag
     7201 gtgccgatcc ccgaaccgct gcgctttatc gagccgcgcg aaacggaaac ccgcaccatg
     7261 cacgcgctgg aggagtacgg cgtgatgcag gtgaagctgt atgaagatat cgcccgcttc
     7321 ggtcatatcg ccaccaccta cgcctatccg gtgaaggtaa acgggcgcta cgtaatggac
     7381 ccgtcgccga tcccgaaatt tgataacccg aaaatgaaca tgatgcccgc cctgcaactg
     7441 ttcggcgcgg ggcgcgagaa gcgcatctat gcggtgccgc cgtttacccg cgtggaaagc
     7501 ctcgatttcg acgatcaccc gttcaccgtt cagcagtgga atgagccatg cgccatctgc
     7561 ggatcgaccc acagctatct tgatgaagtg gtgctggatg acgccggaaa ccgcatgttt
     7621 gtctgctccg ataccgatta ttgccgccaa cagagcgagg cgaaaagcca atgaatcaac
     7681 cgttactttc ggtcaataac ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg
     7741 tctcttttga tttatggccg ggggaagtgc tgggcatcgt cggggaatcc ggctccggga
     7801 agaccacgct gctgaagtcg atctccgcgc gcctgacgcc gcagcaaggg gaaattcgct
     7861 atgaaaaccg ttccctctat ggcatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg
     7921 aatggggcgt ggtgcatcag catccactcg acggcctgcg ccgccaggtg tcggcaggcg
     7981 gcaatatcgg cgagcggctg atggcgaccg gggcgcgtca ttacggcgat attcgtgcca
     8041 ccgcgcagaa gtggctggaa gaggtggaaa ttcccgccaa ccggatcgac gacctgccga
     8101 ccaccttttc cggcggtatg cagcagcgtt tgcagattgc ccgcaacctg gtgacgcatc
     8161 cgaagctggt atttatggac gagccaaccg gcgggctgga tgtatctgtg caggcgcgcc
     8221 tgctcgacct gctgcgcggc ctggtggtgg agctgaacct ggcggtggtg attgtcaccc
     8281 acgatttagg cgtcgcccgc ctgctggcgg accgtttgct ggtgatgaag caggggcaag
     8341 tggtggagag tgggttaacc gaccgcgtgc tcgacgaccc gcaccatccg tatacacaac
     8401 tgctggtgtc atcggttttg cagaattgag ccggtgccgg atgcggcgta aacgccttat
     8461 ccggcctaca aatgcgctcc ccgtaggtcg gataagacgc gtcagcgtcg catccgacac
     8521 ccgaaccacg aggcgaaaaa tgattaacgt acaaaacgtc agtaaaacct tcatcctgca
     8581 tcagcaaaac ggcgtgcgcc tgcccgtctt acaacgtgcc tcgctcaccg tcaacgcagg
     8641 cgaatgcgtg gtgcttcacg gccactccgg cagcggaaaa tcaaccctgc tgcgctcgct
     8701 gtacgccaac tatctgcccg acgaagggca aatccagatc aaacacggtg aggaatgggt
     8761 agacctggtc accgcgccag cccgcaaagt ggtggagatc cgcaaaacca ccatcggctg
     8821 ggtgagccag tttctgcgcg tcatcccgcg tatttcggca ctggaagtgg tgatgcagcc
     8881 tctgctcgat accggcgtac cgcgtgaagc ctgcgccgct aaagccgccc gattactgac
     8941 gcgcctgaac gtgcctgaac gcctgtggca cctggcacca tcgaccttct ccggcggcga
     9001 acagcagcgc gtcaacatcg cccgtggctt tatcgtcgac tatccgattc tgctgctgga
     9061 tgaacccacc gcctcgctgg acgctaaaaa cagcgccgcg gtggtggaat taattcgcga
     9121 agccaaagcc cgtggcgcgg ccatcgtcgg tatcttccat gacgaagccg tacgcaatga
     9181 cgtcgccgac cgcctgcacc caatgggagt ctcttcatga ttatcaataa cgtcaaactg
     9241 gtgctggaaa acgaagtggt gcacggttca ctggaagtac aggatggcaa aattcgcgcc
     9301 tttgccgaaa gccagagccg ccagccggaa gcgatggacg gcgaaggtgg ctggctgctg
     9361 ccggggctga ttgagctgca taccgataat ctggataaat tcttcacccc gcgtccaaaa
     9421 gtggactggc ccgcccactc ggcaatgagt agccacgacg cgctgatggt ggcgagcggc
     9481 atcaccaccg tgctggacgc ggtggcgatt ggcgacgtgc gcgacggcgg cgatcggctg
     9541 gagaatctgg agaagatgat caacgccatc gaagagacgc agaaacgcgg cgtcaaccgc
     9601 gccgagcacc gtctgcatct gcgctgcgaa ctgccgcatc acaccacgct gccgctgttt
     9661 gaaaaactgg tgcagcgcga accggtgacg ctggtgtcgc tgatggatca ctcgccgggc
     9721 cagcgccagt tcgctaaccg cgagaagtat cgcgaatatt atcagggcaa atactcgctc
     9781 accgaagcgc agatgcagca gtatgaagaa gagcagctgg cgctcgccgc acgctggtcg
     9841 cagccaaatc gcgaaaccat cgccgcaatg tgccgcgcgc gacacattgc gctcgccagc
     9901 cacgatgacg ccacgcacgc ccacgttgct gaatctcacc aacttggcag cgtaatcgcc
     9961 gaatttccca ccacgttcga agcggcagaa gcctcgcgca agcatggcat gaacgtgctg
    10021 atgggcgcac cgaatatcgt gcgcggcggt tcgcactccg gcaacgtggc ggccagtgaa
    10081 ctggcgcagc ttggcctgct ggatatcctc tcttccgact actaccccgc cagcctgctc
    10141 gatgcggcat ttcgcgtcgc cgatgacgag agcaaccgct ttacgctgcc gcaggcggtg
    10201 aagctggtga ctaaaaatcc ggcgcaggcg cttaatcttc aggatcgcgg ggtgattggc
    10261 gagggcaaac gcgccgacct ggtgctggcg catcgcaagg gcaatcacat tcatatcgac
    10321 cacgtctggc gtcagggtaa aagggtgttc tgatgacggg aaaactgatt tggttaatgg
    10381 gggcctctgg ctccggaaaa gacagtctgt tgacggaact ccgccagcgg gaacaaactc
    10441 agctactggt agcgcatcgc tacatcacgc gcgccgccag cgccggaagt gaaaaccata
    10501 tcgccctgag cgagcaggag ttttttaccc gcgcggggca gaaccttctg gccttaagct
    10561 ggcacgctaa cggtctgtat tatggcgtcg gcgtcgagat tgacctctgg ctgcacgctg
    10621 gatttgacgt ggtggtcaac ggctcacgcg cccatctgcc gcaggcgcgg gcgcgctatc
    10681 aatcggcgct gctgcccgtt tgtttacagg tttcgccaga gatcctgcgc cagcgcctgg
    10741 aaaaccgtgg tcgtgaaaat gccagtgaaa ttaacgcccg gctggcgcgc gctgcccgct
    10801 atactcctca ggattgtctt acgctcaata atgacggcag cctgcgccag tcggtcgaca
    10861 agttgctgac gctgattcat cagaaggaga aacaccatgc ctgcttgtga gcttcgcccg
    10921 gctacgcagt acgacaccga cgcagtttac gcgctgattt gtgagctaaa acaggcggag
    10981 tttgaccacc aggcgtttcg cgtggggttt aacgccaatc tgcgcgatcc gaacatgcgc
    11041 taccaactgg cgctgctgga tggcgaagtt gtcggcatga tcggcctgca tttgcagttt
    11101 catctgcatc atgtcaactg gatcggcgaa attcaggaac tggtggtgat gccgcaggcg
    11161 cgcggtctga atgtcggcag caaattactg gcatgggcag aagaagaagc ccgccaggcc
    11221 ggggccgaaa tgactgagct ttccaccaac gtgaagcgcc acgacgcgca ccgattttac
    11281 ctgcgcgaag gctacgagca gagccacttc cgcttcacca aggtgctgta acatgagcct
    11341 gaccctcaca cttactggca ctggcggcgc acagggcgtt cccgcatggg gctgtgagtg
    11401 cgtggcctgc gtcagagcgc ggcgctcacc gcagtatcgc cgccagccat gcagcggcgt
    11461 cgtgaagttt aacgacgcga tcaccctgat cgacgccggg cggcacgatc tcgccgatcg
    11521 ctggtcgccc ggatcgttcc agcagttttt gctgacgcac taccatatgg atcacgtgca
    11581 ggggctgttt ccgctgcgct ggggcgtggg cgatcagatc ccggtctacg gcccgccgga
    11641 tgaacagggc tgcgacgatc tattcaaaca ttcgggcctg cttgatttca gccacacggt
    11701 ggagccattt gtgatgtttg atttgcaggg attacaggtc acgccgctac cgctcaacca
    11761 ctcaaaactc actttcggct atttactgga aacggcgcac agccgggtgg cgtggctatc
    11821 tgacaccgca ggcctgccgg aaaaaacgct gaaattttta cgcaataatc agccgcaggt
    11881 aatggtgatt gattgcagcc acccgccgcg cgaggatgca ccgcgtaatc actgtgattt
    11941 aaataccgtg cttgcgctga atcaggttat ccgctcgccg cgggtgattc tgacccatat
    12001 cagccatcag tttgatgcgt ggctgatgga aaacgcgcta ccatcggggt ttgaggcggg
    12061 gtttgatggg atggagattg aggtggcgtg agggaaaata cccgcgccgg cccctatatc
    12121 aacgcccata ctcatcttcc attctccgct cttcatcttc cagttgccga cgctcctgat
    12181 caagctggcg ctggcgatcg tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt
    12241 catcatattg tctgcgccga tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat
    12301 cttcatagtc tcgcccacgg tcagggttat aagcgtcgtt aatcgcctgc tgaatattgc
    12361 caatggtgtc gtcgataata tcggcctggg ccggaacggt ggacagcgtg agcagggtga
    12421 ataaaagaaa tagcggaaag cgtttcatta gccaacctca aaaagaaact ctatccacat
    12481 tagtcattac tcatccatgc aagtagtgga cgaatctcaa tttctccgct gctctattgc
    12541 cgtaatcgcc tccacgcgtt gttgatgacg accgccttcg tactgtgcgc ccagccacgc
    12601 atcgacaatc atttttgcca gttcgaggcc aaccactcgt gaaccaaaag ccagcacgtt
    12661 ggtgtcgtta tgctgccgcg aaagttgcgc ggaataaggt tcgctacaaa cgaccgcgcg
    12721 aattccggca aacttgttcg ccgctatcga aataccgacg ccagtgccac aaatcaaaat
    12781 cccgccatca gcctctccgc cagcaacggc cagtgcgacg tgactggcgt aatgcggata
    12841 atcagtacgc tctgacgacc aggttccttt atcaatcact tcaacgccac gctcaactaa
    12901 atgtgccact atttcatgtt ttaaaatgaa accgacatga tcacagccaa atgcaatctt
    12961 tttcattcat cttccccgcc cgcgcaaaac ttcacaatca aaaaacattt gtgatgtaaa
    13021 tgaattaaaa acacccaaaa tcgatgaaat ccgccttccc gatctgtttt acagagttac
    13081 atctggcagc gcaatagcgt taaagacact cactatccag caacatctat catctaataa
    13141 accaggaaaa caaataacat catgttttta aagtgattaa atgaaatgaa attttaagcc
    13201 actctccatt attcacaaca aaacaaactt tataaatttt atttttttgt gaagttgcca
    13261 gcatcttttc tgttcttgct gtggtgatat agtggcgtct tcaattcaag gacaagagaa
    13321 cgtgatgagc cagtcagagt ttgattcagc gcttccgaac ggtatcgggt tagcgcctta
    13381 cctgcgaatg aagcaggaag gaatgacaga aaacgaaagc cgcatcgtgg agtggttact
    13441 caaacccggt aacctgagtt gtgcacccgc aattaaagat gtcgcagaag ctctggcggt
    13501 atctgaagcg atgatcgtta aggtatcaaa gctgctgggg tttagcggct ttcgtaactt
    13561 acgcagtgcg ctggaagatt atttttctca gtcagaacaa gtattgcctt ccgagttggc
    13621 ttttgatgaa gcgccgcagg atgtggtgaa taaggtattt aacatcactt tacgcaccat
    13681 tatggaaggt cagtcgatcg tcaacgttga tgagatccac cgtgccgccc gctttttcta
    13741 tcaggccaga cagcgggatt tgtacggtgc cggaggatca aatgcaatct gtgctgatgt
    13801 acagcacaag tttttgcgca ttggcgtacg ctgccaggcc tatccggacg ctcacatcat
    13861 gatgatgtct gcttcgttgt tacaggaagg agatgtggtg ctggtggtga cccattccgg
    13921 gcgaaccagt gatgtaaaag cggctgtaga actggcaaaa aagaacgggg caaagattat
    13981 ttgtataacg catagctacc attcaccgat agcgaagctg gccgattata ttatttgctc
    14041 accggccccg gaaacaccgt tattaggtcg taatgcctcg gcaagaatat tacaactaac
    14101 tttgctggac gcgttttttg tctctgtcgc ccagctcaac attgaacaag ctaatattaa
    14161 tatgcaaaaa accggcgcaa ttgttgattt cttctcacca ggcgcgctga aataagaaaa
    14221 aggtaattcc gccatcgcgg agttacctat ccccttaccg tgagaacatg attatgaata
    14281 aatatctgaa atatttcagc ggcacactcg tgggcttaat gttgtcaacc agcgcttttg
    14341 ctgccgccga atatgccgtc gtattgaaaa cactctccaa tccattttgg gtagatatga
    14401 aaaaaggcat tgaagatgaa gcaaaaacgc tgggcgtcag cgttgatatt tttgcctctc
    14461 cttcagaagg cgattttcaa tctcaattgc agttatttga agatctcagt aataaaaatt
    14521 acaaaggtat cgccttcgca ccattatcct cagtgaacct ggtcatgcct gtcgcccgcg
    14581 catggaaaaa aggcatttat ctggtcaatc tcgatgaaaa aatcgacatg gataatctga
    14641 aaaaagcagg cggtaatgtg gaaggttttg tcaccaccga taatgttgcc gtcggggcga
    14701 aaggcgcgtc attcattatt gacaagctgg gtgccgaagg gggtgaagtc gcaatcattg
    14761 agggtaaagc cggtaacgcc tccggtgaaa cgcgtcgtaa tggtgccacc gaagccttca
    14821 aaaaagcaag ccagatcaag cttgtcgcca gccagcctgc cgactgggac cgtattaaag
    14881 cactggatgt cgccactaac gtgttgcaac gtaatccgaa tattaaagcg atctattgcg
    14941 cgaatgacac gatggcaatg ggtgttgctc aggctgtcgc aaacgccgga aaaacgggaa
    15001 aagtgctggt cgtcggtaca gatggcattc cggaagcccg caaaatggtg gaagccggac
    15061 aaatgaccgc gacggttgcc cagaacccgg cggatatcgg cgcaacgggt ctgaagctga
    15121 tggttgacgc tgaaaaatcc ggcaaggtta tcccgctgga taaagcaccg gaatttaaac
    15181 tggtcgattc aatcctggtc actcaataaa actaaatgat gccggatggt gatgctaatg
    15241 aagtgtctta tccggccaac aaaataacct gaacgtagac ctgataagtg tagcccatca
    15301 ggcaataata cgatatttcg tgcagagagg ttatttatgg ccacgccata tatatcgatg
    15361 gcggggatcg gcaagtcctt tggtccggtt cacgcattaa agtcggttaa tttaacggtt
    15421 tatcctggtg aaatacatgc attactagga gaaaatggcg cgggtaaatc cacgctaatg
    15481 aaagttttat ccggaataca tgagccgacc aaaggcacca ttaccattaa taacattaac
    15541 tataacaaac tggatcataa attagcggca caactcggca tcgggattat ttatcaagaa
    15601 ctcagcgtta ttgatgaatt aaccgtactg gaaaatttat atattggtcg tcatctgacg
    15661 aaaaaaatct gtggcgtcaa tattatcgac tggcgagaaa tgcgtgtccg cgccgccatg
    15721 atgttattac gcgtgggctt gaaagttgat ttagatgaga aagtggcgaa tttatctatc
    15781 agccacaagc agatgctgga aattgccaaa acgctgatgc tcgacgccaa agtcatcatc
    15841 atggatgaac ccacctcctc actcactaat aaagaggtgg actatttgtt tctgatcatg
    15901 aatcagttgc gtaaggaggg tacggccatc gtctatatct cgcataaatt ggcggaaatt
    15961 cgccgtattt gcgaccgcta tacggtgatg aaagacggca gcagcgtttg cagcggcatg
    16021 gtgagcgatg tgtcaaatga cgatatcgtc cgtctgatgg taggtcgcga actgcaaaac
    16081 cgttttaacg cgatgaagga gaatgtcagc aaccttgcgc acgatacggt ttttgaggtg
    16141 cgcaacgtca ccagccgtga cagaaaaaag gtccgggata tctcatttag cgtctgccgg
    16201 ggagaaatat taggctttgc cggactggtc ggttccggac gtactgaact gatgaactgc
    16261 ctgtttggcg ttgataaacg cgctggcgga gaaatccgtc ttaatggcaa agatatctct
    16321 ccacgttctc ccctggatgc cgtgaaaaaa gggatggctt acatcactga aagccgccgg
    16381 gataacggct ttttccccaa cttttctatc gctcagaaca tagctatcag ccgcagtctg
    16441 aaagacggcg gctataaagg cgcgatgggg ttgtttcatg aagttgacga gcaactcagg
    16501 cgctttaacg ctcgctgtca tggtctggtg atgggatttc cggcgctggt cagtaaagat
    16561 aaacgcacca ttatttctac gcctaacctg ccgttaacag cggcagattt atatgatctc
    16621 gccgataagc tcgaaaatac gctgaattgt ccggttgagt tttcccgcga cgttaacctg
    16681 caactctcct gggacgtagt agaaaatcgc cttacgcaac aactggttct ggcagcctat
    16741 ctcggtacgg ggatggggtt cgcagtgtgg atgaacggtg cgccgtggac gggtgcgcac
    16801 ggtgtggcag gcgaactggg ccatatcccc ctgggagata tgacccaaca ctgcgcgtgt
    16861 ggcaattctg ggtgcctgga aaccaattgc tctggaatgg cgctaagacg ctggtacgaa
    16921 caacagcccc gaaattaccc attgagcgat cttttcgtcc atgcggaaaa cgcccctttc
    16981 gtccagagtc tgcttgaaaa cgcggcacga gccattgcca ccagcattaa tctgttcgat
    17041 cccgatgcgg tgattctggg tggtggcgtg atggatatgc ccgccttccc acgcgagact
    17101 ctcattgcca tgacccaaaa gtacctgcgc cgtccactgc cgtatcaggt tgtgcgcttt
    17161 attgccgcct catcttctga ctttaatggc gctcagggtg cagcaatatt ggcgcatcaa
    17221 cgttttttgc cacagtcctg tgctaaagtc ccatgagtaa agcgttggca cagtagcgca
    17281 atgtcccgct gtgccaacga caggtacaat atatataata attacataaa acagcccata
    17341 ttattcccag caatgatagt tctactgtac ttttccctct tcagcacaaa tgcctaataa
    17401 aatggcgcgt tcagctcaca cttctactta ttaaagtcgc aatttcccaa ttatgataac
    17461 gccagcttta aatgacgtat tttgcatgga ttacatattt acgacataaa tgataaatgg
    17521 agatttatat gaataactct cggttattcc gtttgagcag gattgttatt gcgttaactg
    17581 ccgccagcag catgatggta aataccgcta atgcaacaga tgaagcgaaa gccgccactc
    17641 aatataccca acaggttaat cagaattacg ccaaatcatt accgtttagc gatcgtcagg
    17701 attttgacga tgctcagcgt ggatttatcg ctccgctgct ggatgaaggt attctgcgtg
    17761 atgcgaacgg taaaatttac taccgcgcga atgattacaa atttgatatt aatgccgctg
    17821 cgccggaaac cataaacccc agcctgtggc gtcagtcgca gatcaacggc atttcaggcc
    17881 tgttcaaagt caccgataaa atgtatcagg tgcgcggcca ggatatctct aacattacgt
    17941 tcgttgaggg cgagaaaggc attattgtta tcgacccgct ggtgacgccg cctgcagcaa
    18001 aagccgcact tgatctttac ttccagcatc gtccgcaaaa accgattgtt gccgttatct
    18061 acactcacag ccacgccgac cactatggcg gcgtgaaagg tattatctct gaagccgatg
    18121 ttaaatccgg caaagtgcag gtgattgctc cggcaggctt tatggacgaa gccatcagcg
    18181 aaaacgtgct ggcgggtaac atcatgagcc gccgtgcgct ctactcttac ggtctgttac
    18241 tgccgcacaa cgcgcaaggc aacgtgggca acggcctggg cgtgacgctg gcaacgggcg
    18301 acccgagcat tattgcaccg actaaaacta tcgtcagaac tggcgagaag atgattatcg
    18361 acggcctgga atttgacttc ctgatgactc cgggtagcga agcgccagcc gaaatgcact
    18421 tctatattcc ggccctgaaa gccctgtgta ccgccgagaa cgccacgcat accctgcaca
    18481 acttctacac tctgcgcggc gcgaaaaccc gcgataccag caagtggacc gagtacctga
    18541 acgaaacgct ggatatgtgg ggtaacgacg cggaagtact gtttatgccg cacacctggc
    18601 cggtctgggg caataagcat atcaatgatt atattggtaa atatcgcgat accatcaagt
    18661 acattcacga ccagaccctg cacctggcga accagggcta caccatgaat gaaatcggcg
    18721 acatgattaa gctgccgcct gcgcttgcca ataactgggc cagccgcggc tattacggtt
    18781 ctgtcagcca caacgcccgc gcggtgtata acttctatct tggctattac gacggtaacc
    18841 cggctaacct gcatccgtat ggtcaggtgg agatgggtaa acgttacgtg caagctctgg
    18901 gcggttctgc ccgcgtcatc aacctggcga aagaagcgaa caagcaaggt gattaccgct
    18961 ggtcggcaga actgctgaaa caggtgattg ccgccaaccc tggtgaccag gtcgcgaaga
    19021 atctgcaagc ggataacttt gaacagctgg gctatcaggc cgagtccgcc acatggcgcg
    19081 gtttctacct gaccggcgcg aaagagctgc gcgaaggggt acataagttc agccacggca
    19141 ccaccggttc cccggacacc attcgcggaa tgtcggtcga aatgctgttc gactttatgt
    19201 ccgttcgcct cgatagtgcg aaagccgcgg gtaaaaatat cagcctgaac ttcaatatga
    19261 gcaacggcga taacctcaac ctgacgctga acgatagcgt gcttaactac cgtaaaacgc
    19321 tgcaacctga agccaacgcc tctttctaca tcagccgtga agatctgcat gccgtgctga
    19381 ccggacaagt caaaatggtg gatctggtaa aagcgaagaa agccaaaatt attggcaatg
    19441 gcgcgaaact ggaagaaatt attgcctgtc tggataattt cgatttgtgg gtgaatatcg
    19501 taaccccaaa ttaatgctac tcacttttgg gggcgcttaa gcccccaaaa gcatttattt
    19561 taaaaatata tacaacaaat taatacataa aacatataag ttatgaatgc ggtggctgaa
    19621 taaatctatt tttattcatc gagaaaatag ccatactgac gccttcaatg gatattgata
    19681 ttcactacag atattgttcg cagcagacag gatgtattta atatgccaac agttctctct
    19741 cgcatggcga tgcaactcaa aaaaacagcc tggataattc ccgtcttcat ggtttcggga
    19801 tgctcattat ctccggcaat cccggtgatc ggcgcttatt atcccggctg gtttttctgc
    19861 gccattgcca gccttatttt gacgctcatc acgaggcgaa ttattcagcg ggcaaatatc
    19921 aatctggcat ttgtcggaat tatttatacc gccctttttg ctctctacgc catgctgttc
    19981 tggctggcat ttttctaatc tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct
    20041 cctcgcagta aattccctgc tctgttagtg gttgcgttgg cgctggttgc ccttgttttc
    20101 gttatctggc gcgtagacag tgcgccatca actaatgacg cttacgcgtc agcagatacc
    20161 attgatgtgg tgccggaagt cagcggtcgc attgtagaac tggcggtcac cgacaaccag
    20221 gcggtgaaac agggtgattt gctattccgc atcgacccac gcccatacga agccaatctg
    20281 gcgaaagccg aagcctccct cgcggcgctg gataagcaaa ttatgctcac ccagcgtagc
    20341 gttgacgcgc aacagtttgg tgccgactcg gttaatgcca cggtagaaaa agcccgtgcc
    20401 gccgcgaaac aggccacaga tacattacgc cgcaccgagc cattactgaa agaaggtttt
    20461 gtctcagcgg aagacgttga ccgtgcaaga actgcgcagc gcgccgcaga agcggatctt
    20521 aatgccgtat tgttacaggc gcagtcagcc gccagcgccg tcagcggcat cgatgcgctg
    20581 gtggcgcaac gtgcggcggt cgaagcggat attgccctga ccaaactgca tctggaaatg
    20641 gcgaccgttc gcgcgccgtt tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa
    20701 tttgcttctg ccatgcgccc tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc
    20761 gccaacttcc gcgaaaccga tctgaaaaat attcgctcag gtacacccgc aaccattcgc
    20821 ctgatgagcg acagcggcaa aaccttcgag ggtaaagtgg attcgattgg ctacggcgtg
    20881 ctaccggatg acggcggcct ggtgctgggc ggcctgccga aagtgtctcg ttctattaac
    20941 tgggtccgcg ttgcccagcg ttttccggtc aaaatcatgg tcgataaacc tgacccggaa
    21001 atgttccgca tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg agcgcgctca
    21061 actccctgcc attaccggtg gtcaggctgc tggcgttctt tcatgaagag ttaagcgagc
    21121 ggcggccagg tcgcgtgccg cagaccgtgc aactctgggt aggctgcctg ctggtaattc
    21181 tgatctcgat gacctttgag atcccttttg tggcgttatc gctggcagtg ctgttttacg
    21241 gtattcagtc gaacgcgttt tacaccaaat ttgtcgcgat cttgtttgtg gttgccacgg
    21301 tgctggagat cggcagcctg tttttgatct acaaatggtc atacggcgaa ccgttgatcc
    21361 gattgatcat cgccggaccg atcctgatgg gctgtatgtt tttgatgcgc acccatcggt
    21421 tggggctggt ctttttcgcc gtcgccatag tcgctattta cgggcaaacc ttccccgcca
    21481 tgctcgacta tccggaagtg gtcgtgcgct taaccctgtg gtgtatcgtt gtcggcctct
    21541 atccaacctt gctgatgacg ttaatcggcg tgctgtggtt tcccagccgc gctatcacgc
    21601 agatgcatca ggcgcttaat gatcggcttg atgatgccat tagccacctg acggacagcc
    21661 tcgcaccgct acccgaaacg cggattgaaa gagaggcgct ggcgctgcaa aaactcaatg
    21721 tcttttgcct cgcggacgat gccaactggc gaactcagag cgcatggtgg caaagctgcg
    21781 tggcaacggt aacctacatt tactcgacgc tgaatcgcta cgatcccacc tcttttgctg
    21841 attctcaggc aattattgaa tttcggcaaa aattagcctc agaaatcaac aagctgcagc
    21901 atgccattgc cgaaggtcag tgctggcaaa gcgactggcg gatcactgaa agtgaagcga
    21961 tggcggcacg ggaatgtaac ctggagaata tctgccagac gttattgcaa ctgggtcaga
    22021 tggacccgaa tacgccgcca acgcccgcca ccaaaccgcc atcaatggtc gccgacgctt
    22081 ttaccaatcc agactatatg cgctacgcgg taaaaacgct gctcgcctgt ttgatctgtt
    22141 acaccttcta cagcggcgtg gactgggaag gcattcacac ctgtatgctg acctgcgtga
    22201 tcgtcgctaa tcccaatgtc ggttcgtcgt accagaagat ggtgctgcgt tttggcgggg
    22261 ccttttgcgg cgcgattctg gcgctgttat tcacgttatt ggtcatgccc tggctggaca
    22321 atattgtcga attgctgttt gtgctggcac cgattttcct gttgggcgca tggattgcca
    22381 ccagctctga acgctcttct tatatcggca cacagatggt ggtcaccttc gcgctcgcca
    22441 cgctcgaaaa cgtttttggc ccggtgtacg acctggtgga aattcgcgat cgcgctctgg
    22501 gtatcatcat tggtaccgtg gtgtcggcgg tgatttacac ctttgtctgg cctgaaagcg
    22561 aagcgcgcac gctgccgcaa aaactggctg gcgcgctggg tatgttgagt aaagtaatgc
    22621 ggatcccacg ccagcaggaa gtcacagctc tgcgcactta tctgcaaatt cgcataggtc
    22681 tgcatgcggc gtttaatgcc tgtgaagaga tgtgccaacg cgtggcgctg gagcgtcaac
    22741 tggacagcga agaacgcgct ttactgattg aacgttcgca aacggttatt cgtcagggcc
    22801 gcgatattct tcacgcctgg gatgcaacct ggaactcggc gcaggcgctg gataacgcac
    22861 tacagccgga cagagcaggt cagtttgccg acgcactgga gaaatacgcc gccggtctgg
    22921 caaccgcact cagtcgttct cctcaaataa cgcttgaaga aacacccgcc tcgcaggcca
    22981 tcctgccgac cttattaaaa caggagcaac acgtctgcca gcttttcgcc cgcttgccag
    23041 actggacagc cccggcatta acgcccgcca cggaacaggt acaaggagcc acgcaatgat
    23101 caatcgtcaa ctttcacgcc tgctgttgtg cagcattctc ggcagcacga cgctgatttc
    23161 cggctgtgcc ctggtacgta aggattctgc gcctcatcaa cagctcaaac cggaacaaat
    23221 caaactggcc gatgatattc atcttgccag ctccggctgg ccgcaggcgc agtggtggaa
    23281 acaactcaat gacccgcagt tggatgccct gatccaacgg acgctaagtg gttcacacac
    23341 cctcgccgaa gcgaaactgc gggaagaaaa agcgcagtcg caggccgatt tgttagatgc
    23401 cggttcacaa ttacaggtcg cagcgttagg gatgctcaac cgccaacgcg tctcggcgaa
    23461 cggcttttta agcccttatg cgatggatgc gccagcactg ggtatggacg ggccgttcta
    23521 tacggaagcc acagtcggtt tgtttgccgg actggatctc gatttgtggg gtgtgcatcg
    23581 ctcagcggtt gctgccgcca ttggcgcgca taatgccgcg ctggcagaaa ccgcagcagt
    23641 agagctatcg ctgaccacgg gcgtagcgca gctttattac agtatgcagg ccagctatca
    23701 gatgctcgat ctgttagaac aaacacgcga tgtgattgat tacgcggtga aagcgcacca
    23761 gagtaaagtg gcgcacggtc tggaagcgca agtgcctttc cacggcgcgc gggcacagat
    23821 tctggcggtc gataaacaaa ttgttgccgt taaagggcaa atcaccgaaa cgcgagaatc
    23881 tctgcgtgca ttgattggcg cgggagccag cgatatgccg gagatcaaac cggtggcatt
    23941 accgcaagtc cagaccggca ttccggcgac actctcttat gagttgctcg ccagacgccc
    24001 ggatctgcaa gcgatgcgct ggtatgttca ggcgtcatta gatcaggtgg attccgcgcg
    24061 ggcgctgttc tatccgagct ttgatatcaa agcgtttttc ggcctggact ctatccacct
    24121 ggatacctta ttcaaaaaaa ccagtcgcca gttcaacttt atcccgggtc tgaaattgcc
    24181 gctgtttgac ggtggacggt tgaatgccaa tctcgaaggc actcgcgccg ccagcaacat
    24241 gatgattgaa cgttacaacc agtcagtact gaatgcggtg cgcgacgttg ccgtcaacgg
    24301 cacgcgtctg caaacgctca acgacgagcg agagatgcag gctgaacgcg tggaagcaac
    24361 gcgctttacc ctgcgcgctg ccgaggccgc ctatcagcgc ggcttaacca gccgcttaca
    24421 ggccaccgaa gcccggttgc cagtacttgc cgaagagatg tcattactga tgctggacag
    24481 ccgccgggtg atccaaagca ttcagttgat gaaatcgctg ggcggcgggt atcaggcaag
    24541 tcccgtcgtc gagaaaaaat aaaatgtctg ccgcgtgatg gctgtcacgc ggtatttcgt
    24601 ttcgtcacgt caaaactgac gacagcctgt ttttcgtcag agttttgaat aaatagtgcc
    24661 cgtaatatca gggaatgacc acaaataaaa tgtggcataa aagatgcata ctgtagtcga
    24721 gagcgcgtat gcgtgatttg attaactgga gcgagaccga tgaaaaaagt cgtcacggtt
    24781 tgcccctatt gcgcatcagg ttgcaaaatc aacctggtcg tcgataacgg caaaatcgtc
    24841 cgggcggagg cagcgcaggg gaaaaccaac cagggtaccc tgtgtctgaa gggttattat
    24901 ggctgggatt tcattaacga tacccagatc ctgaccccgc gcctgaaaac ccccatgatc
    24961 cgtcgccagc gtggcggcaa actcgaacct gtttcctggg atgaggcact gaattacgtt
    25021 gccgagcgcc tgagcgccat caaagagaag tacggtccgg atgccatcca gacgaccggc
    25081 tcctcgcgtg gtacgggtaa cgaaaccaac tatgtaatgc aaaaatttgc gcgcgccgtt
    25141 attggtacca ataacgttga ctgctgcgct cgtgtctgac acggcccatc ggttgcaggt
    25201 ctgcaccaat cggtcggtaa tggcgcaatg agcaatgcta ttaacgaaat tgataatacc
    25261 gatttagtgt tcgttttcgg gtacaacccg gcggattccc acccaatcgt ggcgaaccac
    25321 gtaattaacg ctaaacgtaa cggggcgaaa attatcgtct gcgatccgcg caaaattgaa
    25381 accgcgcgca ttgctgacat gcacattgca ctgaaaaacg gctcgaacat cgcgctgttg
    25441 aatgcgatgg gccatgtcat tattgaagaa aacctgtacg acaaagcttt cgtcgcttcc
    25501 cgtacagaag gctttgaaga gtatcgtaaa atcgttgaag gctacacgcc ggagtcggtt
    25561 gaagatatca ccggcgtcag cgccagtgag attcgtcagg cggcgcggat gtatgcccag
    25621 gcgaaaagcg ccgccatcct gtggggcatg ggtgtaaccc agttctacca gggcgtggaa
    25681 accgtgcgtt ctctgaccag cctcgcgatg ctgactggta acctcggtaa gcctcatgcg
    25741 ggtgtaaacc cggttcgtgg tcagaacaac gtgcagggtg cctgcgatat gggcgcgctg
    25801 ccggatacgt atccgggata ccagtacgtg aaagatccgg ctaaccgcga gaaattcgcc
    25861 aaagcctggg gcgtggaaag cctgcctgct cataccggtt atcgcatcag cgagctgccg
    25921 caccgcgcag cgcatggtga agtgcgtgcc gcgtacatta tgggcgaaga tccgctgcaa
    25981 actgacgcgg aactgtcggc agtacgtaaa gcctttgaag atctggaact ggttatcgtt
    26041 caggacatct ttatgaccaa aaccgcgtcg gcggcggatg tcattttacc gtcaacgtcg
    26101 tggggcgagc atgaaggcgt gtttactgcg gctgaccgtg gcttccagcg tttcttcaag
    26161 gcggttgagc cgaagtggga tctgaaaacg gactggcaga ttatcagtga aatcgccacc
    26221 cgtatgggtt atccgatgca ctacaacaaa acccaggaga tctgggatga gttgcgtcat
    26281 ctgtgcccgg atttctacgg tgcgacttac gaaaaaatgg gcgaactggg cttcattcag
    26341 tggccttgcc gcgatacttc agataccgat caggggactt cttatctgtt taaagagaag
    26401 tttgataccc cgaacggtct ggcgcagttc ttcacctgcg actgggtagc gccaatcgac
    26461 aaactcaccg acgagtaccc gatggtactg tcaacggtgc gtgaagttgg tcactactct
    26521 tgccgttcga tgaccggtaa ctgtgcggca ctggcggcgc tggctgatga acctggctac
    26581 gcacaaatca ataccgaaga cgccaaacgt ctgggtattg aagatgaggc attggtttgg
    26641 gtgcactcgc gtaaaggcaa aattatcacc cgtgcgcagg tcagcgatcg tccgaacaaa
    26701 ggggcgattt acatgaccta ccagtggtgg attggtgcct gtaacgagct ggttaccgaa
    26761 aacttaagcc cgattacgaa aacgccggaa tacaaatact gcgccgtgcg cgtcgagccg
    26821 atcgccgatc agcgcgccgc cgagcagtac gtgattgacg agtacaacaa gttgaaaact
    26881 cgcctgcgcg aagcggcact ggcgtaatac agtcctttct acagcctcct ttcggaggct
    26941 gtttttttat ccattcgaac tctttatact ggttactccc tacccaatcg tattatcaaa
    27001 atgaaaaaaa ttatcgcatt gatgttgttt ttgacattct ttgcccacgc gaacgactcc
    27061 gagcctggca gccagtattt aaaggcagca gaggcagggg accgacgcgc acaatatttt
    27121 cttgccgaca gctggtttag ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa
    27181 gccgccgaca gcggtgatgc tgatgcctgc gcgctgttag cgcagatcaa aatcaccaat
    27241 ccggtcagtc tggactatcc acaagcaaaa gttcttgcag agaaagcggc gcaagcgggc
    27301 agtaaagaag gtgaagtaac gctggcgcat attctggtaa atacgcaagc gggtaaaccg
    27361 gattatccaa aggcaatttc gctgttagaa aacgcctcgg aagatctgga gaacgactct
    27421 gccgtcgatg cccaaatgct gcttggtttg atttacgcca acggcgtggg cattaaggcc
    27481 gacgatgaca aggcaacctg gtatttcaaa cgcagctctg cgatttcccg aaccggttat
    27541 tccgagtact gggcgggaat gatgttctta aacggtgaag aaggttttat cgagaagaac
    27601 aaacaaaagg cgctgcactg gttaaatctg agctgtatgg aagggtttga taccgggtgt
    27661 gaagagtttg aaaaattaac taacggttaa ggtaggcctg ataagacgcg gtaagcgtcg
    27721 catcaggcat tgatgtcgga tgcggcgtaa acgccttatc cgacctacaa aatcacgcta
    27781 aatcaggcta ttgcgcagtc tggcgtaggc ctgataagac gcggtaagcg tcgcatcaga
    27841 tattgatgtc ggatgcggcg taaacgcctt atccgaccta caaaatcacc ctaaatcaag
    27901 ctattgcgca gtctggcgta ggcctgataa gacgcggcaa gcgtcgcatc agatattgat
    27961 gtcggatgcg gcgtaaacgc cttatccgac ctacaaaatc accctaaatc aagctattgc
    28021 gcagtctggc gtaggcctga taagacgcgg taagcgtcgc atcaggcatc cacatattgc
    28081 cgggtggata tccccggcaa tcttcaatta ttgatccgca gttttatcaa atttgcccag
    28141 cacttcgcgc tcataagcca gcgctttctt acggtcaaat ttgtgttccc acttggcaat
    28201 caccagcacc gccagcgcat tacccaccac gttcagcgca gtacgcgcca tgtcgaggat
    28261 acggtcaaca ccggcaataa acgccagacc ttccagcgga atacctacgc tacccagcgt
    28321 tgccagcaac accacaaacg acacgcccgg cacgccagca atccctttcg aggtcaccat
    28381 cagcgtcagc accagaatga tttcctgcca gatggacagg tcaatgccat acaactgcgc
    28441 gatgaaaata gcggcaatac tttgatacag cgtcgaacca tcaaggttaa aagagtaacc
    28501 ggtcggcacc acgaaactgg tgatcgacgc cggtgctccg taggcttcca tcttctcaat
    28561 aattcgcggc agcacgcttt cagagctggc agtggagtac gccagaatca gctcatcttt
    28621 cagaatacga atcaggatcc agacgcttaa cccgcacagg cgcgccacaa ttcccagcac
    28681 taccagcgcg aagaacagaa tggcgaaatg caccagcagc accagtttcg ccagtggcca
    28741 cagagacgag aaaccaaagt tagccaccgt caccgcaatc agcgcaaaca cacccaccgg
    28801 cgcataacgc atcaccatgt gagtcacttt aaacatggtt tcagagatgg agcggaaaac
    28861 ggtcaccagc ggttcacgat gcgtcgcggg cagggatgaa agccccagac caaacagcac
    28921 cgagaagaag atgatcggca gcatttcgcc tttcgccatc gacgccacaa tgttcgtcgg
    28981 caccagcgac aaaatcgtgc ccataatgcc gtgggaactg ctttgtaccg cttccgtagt
    29041 gctctgatat ttcgagatat cgacggtcgc cagctgcgac atatccaccc cggcaccggg
    29101 ctggaagacg ttcgccagag tgatccccaa aatgatggcg acggtggtga tcacctcgaa
    29161 gtaaataatg gttttcgcgc caatacgccc gagctgtttg gcatcaccaa cacccgcgat
    29221 acccaccacc agcgtggaga tcacaatcgg cacaacaatc attttgatca gatggatgaa
    29281 gatatcaccc gccggagaga gcaaattgac gaccagccag tcgcggctgt cgctatggta
    29341 gtgcaggtag cttcccagga gaatgcccag caccatagca aacagaatct gccaggccag
    29401 gctgaatttt atatttttca tcagaataac ttcctcaatg gagcgccgtt ccccgttcag
    29461 aaacgcgaga acgtgacata ctgccagggc atgaattgtg ttgcgttggt ttgtagattt
    29521 ttttaacgct tatgatcagt atcatttgcg cggtatgttg cgcaaccctt taacaacaga
    29581 gcttttcgct gcattaatgc ataataacgc gagtttatgg caggattaat gaataaccat
    29641 ttgttatgga aagattttta tttgtttaat ctgcaataat actgattggt gattattttt
    29701 ggcatatgtt cattcgatta ttttttatgc agctcaataa cataatcttc atcaccagat
    29761 gattaatctg atctccgctg caacaatttc gccatattaa tcgactcgac cagctgtgtc
    29821 cggttgatcc ggggtgagtt gagatccatc actttttgcc atagttcgat ggcctgcgcg
    29881 taattcgcct gcataaacgc atcggaagcc agcagcatca gagcggtgat ttcgttagag
    29941 tccagcgcga gggctttatc gatcattgcg cgtgtctggg cggtcatatg ctggctggcc
    30001 tggtaataaa gcaccgtcgc cagcgccgca tacagttcag cgttctcacc acgtagttgc
    30061 aacgcctgac ggtacgccag cagcgaattg ctgtaatcat tttgccacag atagtactcg
    30121 cccagtaacg cccactgttc gctgttttgt ggattagcgc ggattttatc ctgcaatgcc
    30181 tgaagctgcg cttctggggt ttgctggctg gcaaactgat gcagcggatc gcgctgacgc
    30241 tgatactccg cacgtaccgc ctgccatttt gggcttaaca gataactgcc gatacagaga
    30301 aatgccatca aaatcgtgag tgttgtcagc attttcaccg gtatttgtga ctgtttcatc
    30361 gcttcgccct cactcgccag aggatcagtg ccatcagcag taacaacacc actggcagcg
    30421 cccacaacac cagcgtctgg cccgttaacg gtgggttata gcgaacaaaa tctccgtagc
    30481 gttcggtcat ccagccgatg atttcagctt cgttcttccc ctccgccacc atgctgtaaa
    30541 cctgatggcg catactgacc gccaccggcg cgttggattc cagtaagttt tgattctggc
    30601 actgcggaca acgtaactgg ctggcaatat ttaacgcctg ttgctgttgt tgcggattgg
    30661 cgaattgcca ggtgtctacg acctgagcgc gcgcaaaaca ggtaaataac agcagtagcg
    30721 tgagaagtcc tttattcatc gcgcttcctc ccccgccatc cgcttaacaa tgcgcccgca
    30781 atcatcaaca atcctccccc ccagatccag cgcacaccgc tttgtacata caaacgaaaa
    30841 gcgtaacgat ccgctccggt tttctcgccc atgaccgcat accagtcatg gatgccgttc
    30901 cagcgaattg acggttccat catttgctga cggcgtgctt cataaaaacg ccgctccggc
    30961 gtcagttcac caatgcgttg ctgatggtca aacagcgcca ctatcgcttt ttcgctggtg
    31021 taattgcctc tggcttgcag atcgagtcgc tcaaaacgga aggtgtatcc tgccagcgtc
    31081 acctgctgac ccggctgtaa attcaggctg atctcctgac gactgacgct ggaaaccacg
    31141 atccccgcgg caaataacag cacgccagca tgagctacca gcgccggaag ctgcacgcgt
    31201 ttgccagaga caaacgtcgc cagcacaatc accaccagca tcaacagacc aaacggtaac
    31261 gtcgcgcggt taaaatacgg cgcgccaacg gagaggcgtc cccagcccag cagaccgtaa
    31321 atcatcggat aaagcgttcc caccagcacg atcagtagca ctgcgcaaaa cagcaacagc
    31381 gtagcgagga ttaacatttc ccgcgataac cccgaaaaac gcaccgccgg gccaccgtcc
    31441 cgcgctcgcc agccatacag agccagagac gcaaggctaa tcagtgcaaa caggctgaac
    31501 aacggcacgg cgcggacgtt atccagtgcg aacgcatgaa ccgaaaccag aatgccagaa
    31561 cggacaatta aggtgcccag cagcgacagc atcagagtaa ctatcgccag cagcagcgac
    31621 cagtggcgga aaatcccccg ctggcgtgtc agggacaaac tgtgcagcag cgcagtggca
    31681 gaaagccagg gtaataaaga ggcgttttcc accggatccc agaaccacca gccgccccag
    31741 cccagttcgc aataggccca ccaggaaccg aggatgatcc cagccgttaa tgcactccag
    31801 ccaggtaacg cccagcgcca gcaaattcgg gcgcaggcac catcaaactc gccgcgcagt
    31861 aaactcgcca gcgccacgct cgccgccacc atcaaaccgc catagccaag gtaaagcagc
    31921 ggtggatgaa agataagacc gggatgttgc agcatcggat tgagatcgcg gccttcgatt
    31981 gctggtggaa atatccgcac gaagggatcg gaccacagca ctacaaacag tagcagtgcg
    32041 gcgagcatga gagataaaac ggctagcgtt agcggaaata gcggatcggt ttgctgccga
    32101 taatgccagg caaacagcgc gctccaggcg gaaagcagca gcacccaaag cagcagcgaa
    32161 ccttcatgac cgccccacac cgccgccagt ttgagttccc acgacagcag gctgtagcta
    32221 tgttgggcga cataaatgac cgagaaatcg ctgatgagaa aacaatacgt cagtacgcca
    32281 aaggcgagca gcaggagcgc gaactgcgcc agaatgccga tgcaagtgag tcgcatcatg
    32341 gcaggccagc gcaaccgcac tcccgcgaag gccgtcaacg gggtcaacac gttgaccccg
    32401 agacttaaca acaacgccag aaaaccgact tcaggaagga atacgtccag aaaccacctc
    32461 ttttctggct tacgccaccg tcaactgtcc ggcgtagaga atgaaaaagc gcagcatcaa
    32521 cacgccgacc agactggccc cacacgccgc cagcacggta ggaatgccgg aactgcgatt
    32581 gacccacggt ttgagcaaca ttggcacaat cagccccagc ccggcgacac caagccagaa
    32641 ccaccacgtc cagaaaccgc cacctaatgc cgccaccagc gcacgcactt taccgtcatc
    32701 gcccagcgcc agaccgacaa aaaacgccac cagcaggaag atttcacccc ataccacagg
    32761 gatttccata cggtgaacaa agtgcgcttc cgtggaatgc ggatttttgc gttggcgtat
    32821 cgccatggcg atcagcgcta ccgccgcacc ggacgagatg ccggagaaga ggaacagcac
    32881 cggcaggatc gggttattga ggaacggata cgatttcagc gccgacagca gaaagccggt
    32941 ataagccccc agcagcaccg ccagcaccag catcaatgtt tccagtccgc gatgaacggg
    33001 cgtcagcagg ctcagaacct tttgcacgat ccccagcttc ggcaaccagc gttgttgcag
    33061 ggcaagcaaa tcatgttcaa agattttcgc cagccacagc accagcacca ccatgtagag
    33121 ctgaaacaac atcaccccca tcgacatcac cgaggtaaaa ctgtagtgga acatcagctt
    33181 ccagaaggtc cacggctttg tcaggtggaa gacgaggatc aacagaccga ggatcaccgc
    33241 gccaggcccg acaattagcg tggtgcgcag caacgtactg tctgcaccgc ccgcctgcgg
    33301 gtagaagcga cgtaacagca cggccagcgt caccagaccg gcagaaatac caatcaaaaa
    33361 caggtagatg gcaatcggcc agtcccacac cagcgattca aaatgaaatg cggaagtctg
    33421 catcattggc tcacctcccc gtatttaaac ggtacgcggt acagcttcgg tttggttccc
    33481 agcgccagct tgtagcggta agtgggcttc tggcgcagca gttgcgaaat ctcactgttg
    33541 ggatcgtcca gattgccaaa cgtcagtgcc ttggtcgggc aagcttcaac gcacgcgggc
    33601 agcttaccgg cctgcaaatt ggtcttacgg cagaaatcgc atttgtccgc cgtcttcgtg
    33661 accggatgga taaagcgcac gcggtacgga caggcggcga tgcagtactg acaaccgacg
    33721 cagagatcag gattaacatc gacaatcccg ctagcggcat cacgaaaaga cgcaccggtc
    33781 gggcagacgt caacgcacgg cgcatgatcg cagtgctggc aagacttacg gaagaagcga
    33841 tatttcacat caggaaattc gccctgcggc tcgctacgaa taatcgtcag gcgcgagacg
    33901 ccttccggca ccttgttcac ttcccgacaa gcatccatac aggcggtaca gccgatgcat
    33961 aacgactcgt catgcaccat gccgtaacgc accccattga tattcaacgt tttcgccacc
    34021 acgcgccctg cggtgccgct gacggctgcc agcacgccga cgccggtgag aaactggcga
    34081 cgagaccagg tcatggctgc tccttaagca acggaacaga cgccgggtta aagttcggat
    34141 tggtgcgctg atcgctgtgg caatcgacgc aaatcttaat ccgtcctttg tcacttaacg
    34201 tctgcatcgt atcttgctgt ggatgcaggg aatggcagct ggcgcacgcc actttggtta
    34261 cgtggacatc gtgtggccag aacgcttttt gcaactgttc cggcagatgg caggacatac
    34321 agacgctgtt ctgctctcca accttgtaca tcggctcgtt aaagcgcatc acatctttca
    34381 ccccttcgcg gtgttgcggt gatggctgac cgtggcagtt ggtgcaggtg accggcagtt
    34441 tgttattcgg gttgataacg gaagcatgtt tgccatgcat accttcggta tctggcttat
    34501 gacagtccag acaggcggca tccgggttac gctgctgggt aacttcgtag cgatcaccag
    34561 atgcctgcgc cgcaggggta gcggtaattc cgttcaggct ccacaacagg cccgacgcca
    34621 gcaccccggc agttaataac gaacgtaata cgctcatatt cactccattg tgttttccac
    34681 cccttgcgag gcggaacggg gttattggct taacagaccg tttttacgtg cctgctcttc
    34741 ccactgcggg atcaccgttt tgatgaagtc ctgcttctcg gccttgattt gctccatgtt
    34801 caggccaatg gcctgctggg ctttctcttt ggtcgagata tccgggatct ggatttcatg
    34861 ggtaatgcct ttggtcgcca gcaggcgcgc cagtttggtg cgtgcatccg ccgctttatc
    34921 catcgccgta ccgagcatcc tcagaccttc ttccggtgcg tgcatatgaa tgccgtggga
    34981 agcgatcgcc aaatcccaac gccactgggc atgacggata tcgtcctgaa tcggcttcat
    35041 ttcggcttcc gttgcgcctg catccagcgc cgctttcgct tcgaagtgag cgtgaaccag
    35101 ttgatcttca accttgattt tcaggtcgtt aatcgactgc ttacgttccg cgaccacttt
    35161 ttgcaaggca gctttgtcct gggtatggca gttcgcacaa gtctgggcga agttatcaaa
    35221 cggattacca attttatggt cggtgtagag tttgccttcg gcgttctgca cttttggcat
    35281 atggcaatcg atacaggtca cgttgttttt gccgtgaatg cccgctgtcc aggtttcata
    35341 ttccgggtgc tgcgctttca gcattggcgt tttcgacagg gagttagtcc agtcagagaa
    35401 ggcaattttg tcgtaatact gctccatatt ttcgactttc atgccgtcat cccacgggaa
    35461 tttaaccgct ttgtttttgc cgtcgaagta atactccaca tggcactgac cgcaaaccat
    35521 cgattgctgg tcgaaacgtc cggctttctc aaaaggttta ccaatggctt ccatcgcgcg
    35581 agccgcatac ggacgggaaa gggttaactc cggtttgcct ttggcgaact ctggtgaggc
    35641 ggtgttatgg caatcggcac aacctaagtt gttgacgatt tccgggccgc cgcgcgccca
    35701 ttttccgtgg aagtagccat cttcgccgtc tttctggatc agacgcgcca catctgggct
    35761 tttacaactc cagcatgcca tcggtagcgg accatcttca gcgtttttcg gcgcaccggt
    35821 acgcagggtt tcacgcacat cggtcacagc aaaggcatgt ccacgcggct tgttgtaatc
    35881 gcgcgagaag ggataccccg cccacaggat caccagccgt ggatcttccg ccagggcgtc
    35941 aacacgctct gactgttccg aggtggcttt ccaggagaga tattgatcgg gatgttgcgg
    36001 ggcaaaggtt tcattcttcg cttcaacagt tacaggtttt gcaggagctg ccgtttgttc
    36061 agcgtgaaca gaagtgaaaa agaaaaaagg aatcaataag ctgaagatac ggcgtgcgtt
    36121 tatttttatc cttgtcatag gggcttcatc cgaattgcgc cattgttgca atggcggttt
    36181 ttattgtttt tcacgacagt aaccgcacct acactgtcat gacattgctc gcccctatgt
    36241 gtaacaaata accacactgt gaatgttgtc tttaatcaat tgtaagtgca tgtaaaatac
    36301 cactttagag ttagtcagta tcttcctctt tttcaacagc atgcataact gcatgttcct
    36361 caaagaatta atcaactttt gttgctgacc ttcaaaaatt accctgccgt ttatttgcac
    36421 aattctactt ttgcgtgatc tgtcgcccaa atactaaaca aaactgccaa tacccctaca
    36481 tttaacgctt atgccacata ttattaacat cctacaagga gaacaaaagc atgagtcaaa
    36541 ttcacaaaca caccattcct gccaacatcg cagaccgttg cctgataaac cctcagcagt
    36601 acgaggcgat gtatcaacaa tctattaacg cacctgatac cttctggggc gaacagggaa
    36661 aaattctcga ttggatcaaa ccttaccaga aggtgaaaaa cacctccttt gcccccggta
    36721 atgtgtccat taaatggtac gaggacggca ctctgaatct ggcggcaaac tgccttgacc
    36781 gccatctgca agaaaacggc gatcgtaccg ccatcatctg ggaaggcgac gacgccagcc
    36841 agagcaaaca tatcagctat aaagagctgc accgcgacgt ctgccgcttc gccaataccc
    36901 tgctcgagct gggcattaaa aaaggtgatg tggtggcgat ttatatgccg atggtgccgg
    36961 aagccgcggt cgcgatgctg gcctgcgccc gcattggcgc ggtgcattcg gtaattttcg
    37021 gcggcttctc gccggaagcc gttgccggac gcattatcga ttctaactca cgactggtga
    37081 tcacttccga cgaaggcgtg cgtgccgggc gtagtattcc gctgaagaaa aacgttgatg
    37141 acgcgctgaa aaacccgaac gtcaccagca ttgaacatgt ggtggtactg aagcgtactg
    37201 gcgggaaaat tgactggcag gaagggcgcg acctgtggtg gcacgacctg gttgagcaag
    37261 ccagcgatca gcaccaggcg gaagagatga acgccgaaga tccgctgttt attctctaca
    37321 cctccggttc taccgggaag ccgaaaggcg tgctgcacac taccggcggt tatctggtgt
    37381 acgcggcgct gacctttaaa tatgtctttg attatcatcc gggtgatatc tactggtgca
    37441 ccgccgatgt gggctgggtt accggacaca gttatttgct gtacggcccg ctggcctgcg
    37501 gcgcgaccac gctgatgttt gaaggcgtac cgaactggcc gacgcctgcc cgtatggcgc
    37561 aggtggtgga caagcatcag gtcaatattc tctataccgc gcccacggcg atccgcgcgc
    37621 tgatggcgga aggcgataaa gcgatcgaag gcactgaccg ttcgtcgctg cgcattctcg
    37681 gttccgtagg cgagccaatc aacccggaag cgtgggagtg gtactggaaa aaaatcggca
    37741 acgagaaatg cccggtggtc gatacctggt ggcagaccga aaccggcggt ttcatgatca
    37801 ccccgctgcc tggcgctacc gagctgaaag ccggttcggc aacacgtccg ttcttcggcg
    37861 tgcaaccggc gctggtcgat aacgaaggta acccgctgga aggggccacc gaaggcagtc
    37921 tggtgatcac cgactcctgg ccgggtcagg cgcgtacgct gtttggcgat cacgaacgtt
    37981 ttgagcagac ctacttctcc accttcaaaa atatgtattt cagcggcgac ggcgcgcgtc
    38041 gcgatgaaga tggctattac tggatcaccg ggcgcgtgga cgacgtgctg aacgtctcgg
    38101 gtcaccgtct gggaacggcg gagattgagt cggcgctggt ggcgcatccg aaaatcgccg
    38161 aagccgctgt cgtcggtatt ccgcacaata ttaaaggtca ggcgatctac gcctacgtca
    38221 cgcttaatca tggggaggaa ccgtcaccag aactgtacgc agaagtccgc aactgggtgc
    38281 gtaaagagat tggcccgctg gcgacgccag acgtgctgca ctggaccgac tccctgccta
    38341 aaacccgctc cggcaaaatt atgcgccgta ttctgcgcaa aattgcggcg ggcgatacca
    38401 gcaacctggg cgatacctcg acgcttgccg atcctggcgt agtcgagaag ctgcttgaag
    38461 agaagcaggc tatcgcgatg ccatcgtaac ccacaattgc ccgatgcgag tcggtaacgg
    38521 tttgtaggcc tgataagacg cgacagcgtc gcatcaggcg ctggttgccg gatgcggcgt
    38581 gaacgcctta tccggcctac attcggcaac ggctgtaggc ctgataagac gcgacagcga
    38641 cgcatcaggc gttgattgcc ggatgcggcg taaacgcctt atccggccta cattcggcaa
    38701 cggctgtagg cctgataaga cgcgacagcg tcgcatcagg cgttgattgc cggatgcggc
    38761 gtgaatgcct tatccggcct acattcggca agggttaccc aagcgttaac cttctcccat
    38821 aagggagcgg gaattaaaac aatccctaca ttacctctgg agaatctgtg atgaatggta
    38881 cgatttatca gcggatagaa gacaatgcgc atttcaggga gttagtcgaa aaacggcaac
    38941 ggtttgccac catcctgtcg attattatgc tggcagttta tatcggcttt attttactga
    39001 tcgccttcgc gcccggctgg ctgggcaccc cgctgaatcc gaacaccagc gtcacacgcg
    39061 gtattccgat tggtgttgga gtaattgtga tctcctttgt tctcactggt atctacatct
    39121 ggcgggcgaa cggcgaattc gaccgtctta ataatgaagt cctgcatgag gtacaagcat
    39181 catgaaaaga gttctgacgg cgcttgccgc cacactccct ttcgcagcta acgccgcgga
    39241 tgctattagc ggggccgtag agcgccagcc aacaaactgg caggcgatta ttatgttcct
    39301 gattttcgtc gtgtttacgc tcggcattac ctactgggcg tcaaaacgcg tacgttcgcg
    39361 taatgactac tacaccgcag gcggcaatat caccggtttt cagaatgggc tggcgattgc
    39421 cggggactat atgtccgccg cctcattctt ggggatctcc gcgctggtgt ttacctccgg
    39481 ctatgacggc ttaatttact cgctgggctt cctggtaggc tggccaatca ttctgttcct
    39541 gattgccgaa cgtctgcgta acctgggccg ctacaccttt gccgatgtag cctcttaccg
    39601 cctgaaacaa gggccgattc gtattctttc ggcctgtggt tctctggtgg tggtggcgct
    39661 ttaccttatc gcccagatgg tgggcgcagg taaactcatc gagctgctgt ttggccttaa
    39721 ctatcacatt gcggtggtgc tggtcggcgt gctgatgatg atgtacgtcc tgttcggcgg
    39781 catgctggcg accacctggg tacagattat caaagccgtt ctgttgctgt tcggtgccag
    39841 ctttatggcc tttatggtga tgaaacacgt cggctttagc ttcaacaatc tgttcagcga
    39901 agcgatggcg gtacacccga aaggtgtcga tatcatgaag ccaggcgggc tggtgaaaga
    39961 tccgatctca gcactgtcgc tggggctcgg actgatgttt ggtacggcgg gcttgccgca
    40021 tattctgatg cgcttcttta cggtcagcga tgcccgcgaa gcacgtaaga gcgtgttcta
    40081 cgccaccgga tttatgggct acttctacat cctgaccttt attatcggct tcggcgcaat
    40141 catgctggtt ggtgcgaatc cggaatataa agatgctgcg ggccatctga ttggtggtaa
    40201 caacatggcg gcagttcacc tggccaatgc ggtgggcggc aacctgttcc tcggctttat
    40261 ttcagcggtt gctttcgcca ctatcctcgc ggtggtagcg gggctgacgc tggcgggcgc
    40321 atcggcggtg tctcacgacc tgtacgctaa cgtcttcaaa aaaggcgcga ccgaacgtga
    40381 agagctgcgg gtatcgaaaa tcaccgttct gattttgggt gtgattgcca ttatcctcgg
    40441 cgtgctgttt gagaatcaga acatcgcctt tatggtgggg ctggcgtttg ccatcgcggc
    40501 gagctgtaac ttcccgatca ttctgctttc catgtactgg tcgaaactga ctacccgtgg
    40561 cgcaatgctg ggcggctggt taggactgat tactgcggtg gtgctgatga tcctcggtcc
    40621 gacgatttgg gtacagatcc tcggtcacga aaaagccatc ttcccgtatg aatacccggc
    40681 actgttctct atcagcgtgg cattcctcgg tatctggttc ttctcggcaa cggataattc
    40741 agcggaaggt gcgcgcgagc gtgaactgtt ccgtgcgcag tttatccgct cccagactgg
    40801 ctttggcgtt gagcaaggcc gcgcgcatta atctccacta gcctctggtc acctgaccaa
    40861 cggtaaaaag gtgcgatgta ctgcaccttt ttaccgatta caccgccaaa gcgttattgg
    40921 ttctcttcaa tcagcaacgc ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt
    40981 tcgttactga tctcccgcgt ggcgcgcagg tggtacagtt cagcacgttc agaacgcaat
    41041 gccgccagac ggaagcgacg ctcaaggttc tcttcctgca cggaactttc aacgtcgtta
    41101 cgtccatcgg cgcgacgacg caggttacca atgacgcgag aactaacctc tgtgagcagc
    41161 tggttatcga tattctcttc ggtatccgcc gccagacgct cctccatttt ctggatcgcc
    41221 acaatcgcca cttctgccgt tgccgctcgc gcaatacgtt cctctttcaa ttgttgcgaa
    41281 tggtccgcga cttcaatgtg ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc
    41341 gagaagagaa tgacaccagc cgccaggaac accagctcat agcgcgccgg gaagacgtta
    41401 ccatccggca agagcagcgg gattgagagc acaccagcca gagtgatcgc cccacgcacc
    41461 ccggcgaaag acgcgatcag gatttctcgt gtggtccacg aaccaaactc catcggcttc
    41521 ttcttcagga agcggttgct gaactttttc atcgtccaca gccagccgaa acggaccagc
    41581 atcagcgccg cataaatcag aatgatatcg gtaaacagca tccagatttc gacgttaggg
    41641 tcgatttctg ccgccatcag cgacgtctcc agaatacccg gcagctgcag acctaacagc
    41701 aggaatacca tgccgttaaa gacaaattcc agcatcgccc aggtactgtt tgcgcgcagg
    41761 cgcattgcca gcggcgcacg gcgcatcaca ccggaacggg tgatggtcat cccggcggca
    41821 accgcagcga ggataccgga aacgccaata tgttcggcaa tcagataaga agcgaatggc
    41881 agcaggaaca gcaggacgat ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat
    41941 cgcagcgagc gaccgtacag ccagctcacc acaaaaccgg cgagaatacc gccaatggcc
    42001 actttcataa attcgaccgt cgcaccaccg acggtgaaga tcatcgtccc catcgccact
    42061 gccacggcaa acttcaacga caccagaccg gaggcgtcat tcatcaacgc ttcgccctgc
    42121 aaaatgccca tgattttttt cgggatgcgc ccttcgccaa caatccccga gagcgccaca
    42181 gcatcggtcg gagaaagcac cgccgccagc gcaaaggcgg ggatcagcgg aatgcccggc
    42241 accacccagt aaataagaaa gccaatgccg accacggtga ccaccaccag cgccagcgcg
    42301 aggccgaaaa tctctcgacc atgttcgaga aattcacggg tcggcgtttt ccagccatca
    42361 gcgaacagca acggcgggat aaacaagact aaaaagagtt caggatcaaa ctccacatgc
    42421 aaaccaaacg tcggccacgc cagtagcgca ccgatggcga tttgcataag cggaagcggg
    42481 atctgaaagg gcatgacacg agtgaccacc ccggacagcg agaccacgag ggtcattatc
    42541 agtatggtga agaagatttc catgcgttcc ctgtttgcga tgttttttat gatctgagaa
    42601 aggcgttatg cctgaattct cattttttca gaatatcgca aaagctcaca caatgtgcgg
    42661 agaaaataaa aacgggcagc cattgccacc cgttttcaga atcatatgaa agattagata
    42721 gcccagccac ccgcatagaa ggtcaccagc gccacagcga taacgaccgt accgatgttc
    42781 aacttgcgcc attcgccgga aaccagacga ccaatcacca gagtcgcgaa gccgatcatg
    42841 atgcctgtta cgatgttaca ggtcagcacg atgaataccg ccgtaaccag acccgccatc
    42901 gcatcaacaa aatcagcaaa gtcgattttc gccacgttac tcagcatcag caggccaacg
    42961 tacatcagcg ccggagccgt tgcatacccc ggaacgaggt aagagagcgg agagaggaac
    43021 agaatcagca ggaacagcac gccaacggtg atagccgtca aaccggtttt accgcccgcc
    43081 gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac caggccagag
    43141 aaaacgctgc tcatggagtc agtggtcagt gctttcccgc cgtcgatgat ctgcccatct
    43201 ttatccagca ggttcgcctg gccggcgacg gcacggatag ttccggtggc atcaaatact
    43261 gccgtcatca ccagcgccag aacgcttggc aggactacag gattcagcgc gcccataatg
    43321 tccaggctgc caattagtga attgccgttt tcatcgctca atgaaggcat ggcgaaaacg
    43381 ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc gataatggtc
    43441 agcagaatgc caccagggac tttcagtttt tccaggccga taatcactgc cagccccacc
    43501 aacgacatca tcaccggaaa ggtcgtgaaa tcccccagcg cgacgggcag accatcaagt
    43561 gggtttttaa tcaccagacc gacaccatta gcagcaatca gcagcaggaa cagaccgata
    43621 ccaatccccg tgccgtgcgc cacaccgtgc ggcaagttgc gcaaaatcca gctacggata
    43681 cccgtggcag aaatgaccgt aaacagcaca cccatcagga acacggcacc cagcgcgaca
    43741 ggtacgctaa tatgttgccc cagcaccaga ctgaatgcgg taaacgccgt cagggagatg
    43801 gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga accgagtccg
    43861 gcaaccagac aggtcgcaac gaaaactgcc gcaggcggga agcctgcttt acccaacatg
    43921 cctggaacga cgataaccga gtagaccatc gccagaaatg ttgttaaccc ggcaaccact
    43981 tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg
    44041 gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt ttcgtcaaga
    44101 tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg tttgcgacaa
    44161 attggtgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa
    44221 aagcaaacga ttatccggcg atcatagcgg ctttttcaac cgaagtttgc tgcttttttc
    44281 tcttttattg tcttgacgat gaaaaaccag gcaattgatg cgcaaacgtt atcgtcgatg
    44341 cgcaagattg cagcgttatc gagccgtata ccgttcagga ttgcgataaa aaatcgtctg
    44401 agatgacaaa aatattgcgg ctaaaaagtg tacttttaca ctggcgaaga aacgccggtg
    44461 acatacaatt aaagcatcaa caccaaccgg aacctccacc acgtgctcga atgaggtgtg
    44521 ttgacgtcgg gggaaaccct cctgtgtacc agcgggatag agagaaagac aaagaccgga
    44581 aaacaaacta aagcgccctt gtggcgcttt agttttgttc atcttccagc aagcgtgcgc
    44641 ctgtgccttc ttctcctaag cggtcgcccg ggttacgcaa tgggcaatca ctgcgcgaaa
    44701 ggcagccaca accaatacat ccgtccagct cgtcacgcag cgccactaag gtatgaatgc
    44761 gccgatccaa ctcttctcgc cattgggacg aaagctgttt ccactctttc gcacttaatg
    44821 tatgcccttc gggcaacacg ccaaatgctt cacgaatggt cgccagcgga atgccaatac
    44881 gctgagcaat tttgatgatt gcgacatatc gcaacacatc acgtttatat cgccgctgat
    44941 tgccactatt acggatactg ctaatcaacc ctttgctttc atagaaatgc agcgccgata
    45001 ccgccacacc gctgcgtttt gccacttcgc cgggggttag cagcgcttta atgcggggta
    45061 atttcttttc cataaatcgc tttacctcaa gttaacttga ggaattatac tccccaacag
    45121 atgaattaac gaactgaaca ctgaaaagag gcagatttat gtcccatcag aaaattattc
    45181 aggatcttat cgcatggatt gacgagcata ttgaccagcc gcttaacatt gatgtcgtcg
    45241 caaaaaaatc aggctattca aagtggtact tacagcggat gttccgtact gtgacgcatc
    45301 agacactagg tgattacatt cgccaacgcc gcctgttact ggccgccgtt gagttgcgca
    45361 ccaccgagcg tccgattttt gatatcgcaa tggacctggg ttatgtctct cagcagacct
    45421 tctcccgcgt tttccgtcgg cagtttgatc gcactcccag cgattatcgc caccgcctgt
    45481 agttttattg cccgcgcgtt aactcccgcg cgggtaattg ctccatccat tgcataaaca
    45541 cctgcggcgg catcgccttc gcaaagaacc atccctgaca atagcgcacg ccgcgtttgc
    45601 gcagccagtt aacctgctcc tcagtttcga cgccttcagc gatcgttttt aaccccagac
    45661 tgtgcgccag ctcgatgata tgttccgcaa tcaaatgact ggttttatgg gtggtcagcg
    45721 tctcaacaaa cgatttgtcg attttcaaaa tatcgacatt caatgattta aggttatgca
    45781 agttagagta gccaatacca aaatcatcaa ttgccacttc gtaaccagcc tggcggaaag
    45841 ccagaataat cggcgtcatt ttgtcgacat caagaaatgc atgttcagtc acttcaaatt
    45901 taatttgctg cggacgcacc gcatattgct ctgttttctg gttggttcgc gctatcaacc
    45961 gtgacgtatg aaaatcggag gccgacaggt taatagaaac atagcgatcg acatgtgttg
    46021 ccaggtacac gcccagatcg cggaagacat tatcaataac ataatcagtt atctgttcga
    46081 tcatcccctc cttttctgcc agcggaataa actctgccgg attcattact tgcccctgct
    46141 caccaggcca acgtaacaat gcttcagctc cgatacattt ttctgttttg atatcgatta
    46201 ttggctggta ataaaggcaa agttgatgtt tttcgagggc gcgttgcaat ttgcgtttgg
    46261 gagacaaata gttttgtcga atacgtagcc agagtaatag cagaacaaga ctccccaaaa
    46321 taccggcggg caacgcaaaa ataagatgat tataaaaatg agttataaga cgttgatatg
    46381 aagtcgcaac aatggctgca attgggcgtt ttgtcgaata aactgtcgca tataaatagc
    46441 catttcgttg tacggttaaa tcattcaaat gaatcagcgg agaaaacgtt gctgccgagg
    46501 cctcgttgct taacgagaaa aaggttttcg tcaccgtatc atacactccc cattgcaatg
    46561 tcggatcatc agacatcact tcactccaga agagagggtt gataaccgcc acataatttc
    46621 cccgctgcat gtaggtcatt ttatagccag agaaaaaagg cgtatcacgg taataataga
    46681 tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat cgtatagcca tttacagacg
    46741 ctatcagcga tgagcataaa aaatggttat cacgggcata gatcaattca ttaatataaa
    46801 gatagccacg aataatattc aacattcgct tttgatgggc tggagtgcaa acttgcccct
    46861 gatagcgttc tgctgcatcg ctcactaaat ctgcctggcg aatgaccagc tcggatttat
    46921 ccagcgccag ctgagcaaaa gtttggagct gactattcac ttctgatttt gcccaaagga
    46981 aggcaatcca tagcgacaga atgatgggaa agagaactaa aaagataatg cccggcaacg
    47041 ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa tttcctgcct gtttataccc
    47101 ggccattgca aggtgaaaac cctgggcacc gacgtcttta gataaatctt acggtataaa
    47161 tagcactttt gcctgatttg aaattgatac ctttttaatg gtcacctgga taaaaataat
    47221 tcattgatat tcaaaactaa ttttctacag gaatgcagag gcggcgggaa gatatgccct
    47281 taatgccact gaaaaaccac ataatctgta cagcaataaa agtcacggcc taatctggct
    47341 aacaaacaat caattgtgat atagttcaca aaattaatga aacaaacaga gtgtttcatt
    47401 tttgtgtcct gcaagccccg cgatagccag agtgtaccac gcctcccgtg aacaacgccg
    47461 cgctgtccag ggcatcggct cttttcagag gaattgtttg atggctaccc tcaccactgg
    47521 cgtggttcta cttcgctggc aacttcttag tgccgtaatg atgtttctgg ccagcacgct
    47581 caacatccgt tttcgtcggt ctgattatgt cgggcttgca gtgatcagca gcggtctggg
    47641 cgtggtttct gcctgctggt tcgcaatggg gttgcttggc atcacaatgg cggatatcac
    47701 cgccatctgg cacaacatcg agtcggtgat gattgaagag atgaatcaga caccgccaca
    47761 atggccaatg attttaactt gatatgtaga agcctccata atggaggctt ctttttttat
    47821 ggctggcaga tgctttaatc atccacctta aaacaatata acctattgtt ttaatgacaa
    47881 atcagaacgg aatatcgtca tcaaaatcca tcggcggctc gttagacggt gctgccggag
    47941 cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccgccc tgcggctgct
    48001 gaggctgacc ccaaccgccc tgcggctgac caccaccgat attgccacct gcaggagcgc
    48061 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
    48121 tggtgtagcg atcctgaccg gattgatcgg tccatttgcg ggtacgcagc tgaccttcga
    48181 tataaacctg agaaccttta cgcagatatt cgctggctac ttctgccagt ttgccgaaca
    48241 gcacaacgcg gtgccactca gtctgctctt tcatctcgcc ggtcgctttg tcacgccagg
    48301 attcggaagt agccagcgta atgttggcaa cagcgccacc atttggcatg tagcgtactt
    48361 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
    48421 tcgtgtctcc tgaaaaaaaa tcgttctgaa taagtgtaaa cgcgcgattg taccattacc
    48481 aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa caaacctggc cagacattgt
    48541 tacacaacac tccgggtaat gcattccaat actgtatatt cattcaggtc aatttgtgtc
    48601 ataattaacc gtttgtgatc gccggtagca ccatgccacc gggcaaaaaa gcgtttaatc
    48661 cgggaaaggt gaatggataa gatcgaagtt cggggcgccc gcacccataa tctcaaaaac
    48721 atcaacctcg ttatcccccg cgacaagctc attgtcgtga ccgggctttc gggttctggc
    48781 aaatcctcgc tcgctttcga caccttatat gccgaagggc agcgccgtta cgttgaatcc
    48841 ctttccgcct acgcgcggca gtttctgtca ctgatggaga agccggacgt cgaccatatt
    48901 gaggggcttt ctcctgccat ctcaattgag cagaaatcga cgtctcataa cccgcgctct
    48961 acggtgggga caatcaccga aatccacgac tatttgcgtc tgctgtacgc tcgcgttggt
    49021 gagccgcgct gcccggacca cgatgttccg ctggcggcgc aaaccgtcag ccagatggtg
    49081 gataacgtgc tttcgcagcc ggaaggcaag cgtctgatgc tgctcgcgcc aatcattaaa
    49141 gagcgcaaag gcgaacacac caaaacgctg gaaaatctgg caagccaggg ttacatccgt
    49201 gctcgtattg atggcgaagt ctgcgatctt tccgatccgc cgaaactgga actgcaaaag
    49261 aaacatacca ttgaagtggt ggttgatcgc ttcaaggtgc gtgacgatct cacccaacgt
    49321 cttgccgagt cgtttgaaac cgcgctggag ctttccggtg gtaccgcggt agttgccgat
    49381 atggacgatc caaaagcgga agagctgcta ttttccgcca acttcgcctg cccaatttgc
    49441 ggctacagta tgcgtgaact ggaaccacgc ctgttttcgt ttaacaaccc ggcgggtgcc
    49501 tgcccgacct gtgacggcct tggcgtacag caatatttcg atcctgaccg cgtgatccag
    49561 aatccggaac tgtcgctggc aggtggtgcg atccgaggct gggatcgccg caacttctat
    49621 tacttccaga tgctgaaatc gctggcagat cactataagt tcgacgtcga agcgccgtgg
    49681 ggcagcctga gcgcgaatgt gcataaagtg gtgttgtacg gttctggcaa agaaaatatt
    49741 gaattcaaat acatgaacga tcgtggcgat acctccattc gtcgtcatcc gttcgaaggc
    49801 gtgctgcaca atatggagcg ccgctataaa gagacggaat ccagtgcggt acgtgaagaa
    49861 ttagccaagt ttatcagcaa tcgtccgtgc gccagctgcg aagggacgcg tctgcgtcgg
    49921 gaagcgcgcc acgtttatgt cgagaatacg ccgctacctg ctatctccga catgagcatc
    49981 ggtcatgcga tggaattctt caacaatctc aaactcgcag gtcagcgggc gaagattgca
    50041 gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc tcgttaacgt cggcctgaat
    50101 tacctgacac tttcccgctc ggcagagaca ctttctggcg gtgaagccca gcgtatccgt
    50161 ctggcgagcc agattggtgc gggcctggtt ggcgttatgt acgtgctgga cgagccgtct
    50221 atcggcctgc accagcgcga taacgagcgc ctgctgggta cgcttatcca tctgcgcgat
    50281 ctcggtaata ccgtgattgt ggtggagcac gacgaagacg cgattcgcgc cgctgatcat
    50341 gtgatcgata tcggtccggg tgcgggtgta cacggcggtg aagtcgtcgc ggaaggtccg
    50401 ctggaagcaa taatggcggt gccagagtcg ttgaccgggc agtacatgag cggtaaacgc
    50461 aagattgaag tgccgaagaa gcgcgttccg gcaaatccgg aaaaagtgct gaaactgaca
    50521 ggcgcacgcg gcaacaacct gaaagacgtg acgctgacgc tgccggtggg tctgtttact
    50581 tgcatcacag gggtttcagg ttccggtaaa tcgacactga ttaacgacac actgttcccg
    50641 attgcccaac gccagttgaa tggtgcgacc atcgccgaac cggcaccgta tcgcgatatt
    50701 cagggactgg aacatttcga caaagtgatc gatatcgacc aaagcccaat tggtcgtacc
    50761 ccacgttcca atccggcaac ctataccggc gtgtttacac ctgtacgcga actgttcgca
    50821 ggcgtaccag aatcccgtgc gcgtggttat acgccagggc gtttcagctt taacgtccgt
    50881 ggcggacgct gcgaggcctg tcagggcgac ggcgtgatca aagtggagat gcacttcctg
    50941 ccggacattt acgtaccgtg cgaccagtgc aaaggcaaac gctataaccg tgaaacgctg
    51001 gagattaagt acaaaggcaa aaccatccac gaagtgctgg atatgaccat cgaagaggcg
    51061 cgtgagttct ttgatgcggt gccagctctg gcacgtaagc tgcaaacgct gatggatgtt
    51121 ggcctgacgt acattcgcct cgggcagtcc gcaaccacac tttctggtgg tgaagcccag
    51181 cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg ggcagacgct gtatattctc
    51241 gacgagccga ccaccggtct gcacttcgcc gatattcagc aactgctcga cgtactgcat
    51301 aaactgcgcg atcagggcaa taccattgtg gtaattgagc acaatctcga cgtgatcaaa
    51361 accgctgact ggattgtcga cctgggaccg gaaggcggca gtggcggcgg cgaaatcctc
    51421 gtctccggta cgccagaaac cgtcgcagag tgcgaagctt cgcatacggc gcgcttcctc
    51481 aagccgatgc tgtaatcgtt aaggccgctt tcagagcggc cttcttcttt cagagttgca
    51541 ccagcaattt acgtttttct tccggcagca aattcaccgc ctgctgataa gacgcatcca
    51601 ccagataata gatttgcgaa tccggcagcg aaccgtcgag atacacggtg ctccagtgcg
    51661 ctttattcag atggcggctt ggacgcacat cgctgtgctg ctgacgtagc agctccgcca
    51721 gctccgggct ggttttcagc gaaacagctg ggcgattttc tacttctttc accatcgcaa
    51781 acagcacatc ttccactttg atctgcgtcg ctttccagtc gttatgcacg ctctgttctg
    51841 cgcctggttt tgccatgcaa tattgtagca actccgaaat ggtcattttt cactcccctt
    51901 gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg atgttccccc agccagatgc
    51961 cttgccaggt gccggtctga atacgccctt tatgcaccgg caatacaagc gatgttccca
    52021 gcattgagga tttgatatga gaaggcatat cgtctgctcc ctcatagtca tgctcataat
    52081 ttccgttatc gggaacggcg cggaggaaaa aacgctccat gtcgtggcgt acggtgggat
    52141 cgcagttctc attaagtgtc agagaggcgg aggtatgttg cagcaacaga tgcagtaaac
    52201 cgatgttaac gcgcggcata tcagccagct gattcagaat ttcatccgtt accagatgaa
    52261 acccacgaga tttggcgcta agcgtgagcg tcttttgata ccacatgtgc tgctccttga
    52321 taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg ataaaggtgt gattaaaaaa
    52381 acagcattcg ggagagtatt acgctctccc gctctgtcag tattctgaat tgacgatcac
    52441 ctcttcacca aacgcacccg cttgtggcaa gggtttgtag gtagagttgg aggcgcgcag
    52501 aatgcggata ccacgagcgc cgacatcgcg tgcggcggta atatcgttat cggaatcacc
    52561 ataaaaaatt cggatatttt tatcctgcag ccattgcgat tttgtatttt gccctggttt
    52621 atcgcccgca aagatcaccg gattcatgtt ggtggcagga atatgaaaat tatccgccag
    52681 cgtttttgaa accgtttctg ttttggtcgg gctacgacca gtcacaaaga agatcgcgtc
    52741 accgcggcgt acatgcatat caatcagctg gcgagcgacc tcttttggaa tgctgaattc
    52801 atcccagcca ttgttcattt tttcccagaa cacaggattt ttcagataat cttcgctttc
    52861 tggcgagaag gtttttttgc cgcgccagaa gcccggactg gaaaaaagta ccgtgtcatc
    52921 gatatcaaac cccaccgcca ttggcggacg ccctgcgagg ctattttcaa tttgtgcgac
    52981 cgaaacccaa tgaattggtg cctgttcagc aagcctggca acgttagtac cagggttaag
    53041 cggtgaagga gatgaggcca gggcaacagc ggaactgttt agagcgaaca ataagcaaac
    53101 ggcactgatt gcctgtgtga tcttgcgcat atttttccct aaatagtcag tatgttgaaa
    53161 ctttttattg tgagatttgt tgcaaaaacc atctgaccat aacgccagca gccggggaaa
    53221 ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg attatattta acccagtaaa
    53281 tacgttcctg tcgttttttg taaaaaaaat gtttattacg cgatgtaaga atacattaat
    53341 tttgcgttta ttatgatgaa tcctctgcaa atgagcgaaa tttgatagaa catttatctg
    53401 ctgtacgatt taataaactt aatgatacag cattacaaaa caatcgaagt ttataaagat
    53461 gatttctgat tgaccagccc cctaccctac aatggtgact aattttatta ctcccgaagg
    53521 agatgatgat atgaatatat caaatgtgaa tagcaacaac acgacatctt tacctgtaga
    53581 gctagataca ctaaataaca aaggtatctc ttatgccaaa gatttctctt atgccaaaga
    53641 tctcttttta tatatagaaa cacagttaaa aattgcaaaa gatttttgta gacctggaga
    53701 agaagtatca agttctattg caagtacagt ttttcacgca tttattgatt tagttaacaa
    53761 aatcaggggt aagaaagatt gtatgtatat ttgcacgctt tgctgttttg ccgaggaggt
    53821 taaaggtgat tattctcatt acaggacctt tttatttgat attggtaatc aatataaggt
    53881 taaacttaca caaagcggaa aaaaagagtt ctctttaact ttagaattta acgatactat
    53941 aattgaatct cagatagtca caggcaataa ggcaaagcat attcttgagg atatagaaaa
    54001 attctatcgt aacaaacccg atacttatta ttaaatttaa taatggttag atgtgcaaaa
    54061 attattattc cacacggtaa aacagggaaa agtactccag aacattggga taaacttaac
    54121 aagttagaac taacaacgtt ttattacctc tcacgtagaa cgatggcatc aaaaaaatca
    54181 tctataaagc aagagtgaaa gcgacgacag tttaatttca ctgcaggctg gataactcca
    54241 gcctgctttc ctgcattaca tcaccgcagc aaacgccttt gccacacgtt gtacatttgc
    54301 cgtatttaac ccggcgacac acatgcgacc gctggcgatg agatagacac caaattcttc
    54361 acgtagtcgg tcaacctgag cggcacttaa accggtataa ctgaacatac cgcgctgatt
    54421 aagcagataa tcgaaattgc gttctggtat ctctgtgctc aataccttca ccagttcctg
    54481 acgcatcgcc agaatgcgag tacgcatctc ttctacttcc gccagccagc tggctttcaa
    54541 tgcctcgtca ttcagcaccg cagccaccac ctgcgcacca aaattcggcg ggctggagta
    54601 gttgcggcga actgttgctt tcaattgccc cagtacgcgg cctgcggctt cggcatcttc
    54661 acacagaaca gaaagtccgc cgacgcgctc gccgtaaagg gagaaaattt tcgagaacga
    54721 attgctcacc agagcgggta atccagcgct ggcaatggcg cgaatggcgt aggcatcctc
    54781 ttccataccg gcaccaaatc cttgataggc aatatcgagg aatggaataa gctcgcgggc
    54841 tttgagaatt tcaatcacgg aatcccactg gtcattagtg agatcggcac ccgttgggtt
    54901 gtggcaacat ggatgcagca acacaatact gcgggcaggt aatgttttca gcgtcgccaa
    54961 caggtcatta aagcgcacgc cgttagtcgc ttcgtcatac caggggtaag tgctcacttc
    55021 gaatccagcc ccggcgaata ttgctacgtg gttttcccag gtaggatcgc tgacccagac
    55081 gcctgattcc gggaagtagc gtttcaggaa atctgcaccc actttcaatg cccctgagcc
    55141 gccaagggtt tgaatggttg ctacgcgctg ttgttgcagt accggatggt ccgcaccaaa
    55201 cagcagcggc gcaatggcat ggcgatagct gttaagccct tccatcggta aataaagcga
    55261 agcaccatga ggctgcgcat tcaggcgcgc ttccgcatcc gctacggctt tcagttgtgg
    55321 aataattccg tcttcgttgt agtacagacc gatacttaaa ttcactttgt cgctgcgagg
    55381 gtcttcttta aaacgctcca taagcgtaag aatcgggtcg ccagcgtagg cgtcaacttt
    55441 ttgaaacacg cgatggttct ccaggtttac gggcaggtgg ttaaaacaca ataaaccgga
    55501 agaaggcgaa gatcgagagg atgttcaggg gcgaatggca attagcaaca gagtgagact
    55561 catgacaaac gtacatccgc cagatgcacg acctttataa gagcatgaaa gaatatcaac
    55621 ttattgaatt ggtaagattt tattggccgg ataaggcatt cacgccgcat ccggcacaga
    55681 caatcaaata ttacagaacg attaatccac gtatttcatc gcgacccttg aagtcaggcg
    55741 cgtaataagt tcgtaagcgc tcacttttgt catttcagcg atacgttcta cgggcaaacc
    55801 ttcgccccat aaaatgaccg ggtccccggc tttgtcctgc gcctgtggac ctaagtctac
    55861 gcagatcata tccatcgcga cgcgcccgac aatcggcact tcgcgaccgt tcaccagcac
    55921 tggcgtaccg gacggcgcgg cgcgcggata accatcgcca tagcccatcg cgactacgcc
    55981 aaggcgggta tcacgttcgc ttacccaggt tccaccataa ccgacaggct ctccggcttt
    56041 atgctcacgc acagcaatca ggctggaggt caatgacatt accggctgac agccaaaatc
    56101 ggcaccggtg gaacgatctt ccagcggcga gacgccataa agaatgatgc ccgggcgcac
    56161 ccagtcaaaa tgcgactgtg gccacagcag aatgccaccc gacgcggcaa tggaacgttg
    56221 acctggtttg ccttcacaaa aggtattaaa gatagcgagt tgtttctcgg ttgcgccgca
    56281 ttttggttca tccgcgcgcg caaaatggct gacgatattc accggctgac gaacgttttt
    56341 gcactgggtc aggcgatgat aaaacgcctc agcctgttcc ggccttacgc ccagacggtg
    56401 cataccggta tcgagtttca tccagacggt aaccggctcg tccaggctag cctcttccag
    56461 cgcagccagc tgttcttcgt tatgcacggc ggtatgaaaa tgttgcgcag aaatcgtcgg
    56521 cagatctctg gcatcaaaaa agccttcgag taacagtaca ggtttggtga ttccccccgc
    56581 acgcagtcgc agagcttctt cgagacgggc tacgccaaag gcgtcagcat cggggagcgt
    56641 tcgcgcggtc tcaagaagac cgtgaccata agcgttcgct ttcaccaccg caaccatttt
    56701 actggcagga gccagttcac gaagacgttg caggttgtgt cgcagagcgc ggcggttaat
    56761 cacaacagtt gccgcttgca tttgtgttcc ttgataagtg tttgctttaa ttacctaatt
    56821 cataaaataa ttattattcg tcgtcgtact gcggccccgc atagttgtcg aagcgcgacc
    56881 attgaccgtt aaaggtcagg cgtaccgtcc cgattgggcc gttacgttgt ttaccgataa
    56941 taatttccgc gatgcctttt aaatcactgt tttcgtgata cacctcatca cgatagataa
    57001 acatgatcaa gtccgcatcc tgctcgatag agccagattc acgcaggtcg gagttgaccg
    57061 ggcgtttgtc ggcacgttgt tccagagaac ggttcaactg ggacagcgcc accaccggca
    57121 cgttcaattc tttcgccagt gctttcagcg agcgggagat ttctgcaatt tccagcgtac
    57181 ggttatcgga aagcgccggt acgcgcatca gttgcaggta gtcgatcatg ataagcccga
    57241 tgccgccgtg ttcacgggca atacggcgcg cgcgggaacg cacttccgtt ggcgtcaggc
    57301 cggaggagtc atcgatatag atattgcgtt tttcgagcaa aatacccatg gtgccggaaa
    57361 tgcgcgccca gtcttcatca tcgagctgcc cggtacggat tttagtctgg tcaacgcgcg
    57421 acagcgacgc cagagaacgc atcataatct gttctgatgg catctccagc gagaagataa
    57481 gtactggttt atcctgcaac atcgccgcgt tttcgacgag gttcatcgca aatgttgttt
    57541 tacccatcga cggacgcgcg gcgacgatga tcaaatccga cggctgcaag ccagcggttt
    57601 ttttgttgag atcgtcataa ccggtgttta ccccggtaac gccatcgtgt ggctgctgaa
    57661 acaactgctc aatacgcgcc accgtggcgt cgagcacatc agcgatgttc ttcggccctt
    57721 cgtctttgtt tgcacgactt tcggcaattt taaagacgcg ggattcagcc aggtccagca
    57781 gatcttcgct ggtgcgcccc tgcggatcaa aaccggcttc ggcaatctca ttcgcaaccg
    57841 aaatcatctc gcgaacaacg gcacgttcac gcacgatgtc agcataagca ctgatgttcg
    57901 ccgcacttgg cgtatttttt gacagctctg ccagataagc aaaaccaccg acgctatcga
    57961 gttgcccctg gcgttccagc gattccgcaa gggtaatcag atcgatagga ctaccgcttt
    58021 cctgcaaacg cgccatttca gtaaagatat gacggtgtgg gcgggtgtaa aaatcatctg
    58081 ccaccacacg ctcagctaca tcatcccagc gttcgttatc cagcattaaa ccgcccaaca
    58141 ccgactgctc cgcttcgatc gagtgcggag gcactttcag cccggcaact tgtggatcgc
    58201 gttcgcgggg ttcagcctgc tgtttgttga agggtttatt tcctgccata gtgaatggag
    58261 ttaccgagat aaagaatggg tcgaaacttt accatatgaa gcagaccctg acgatacgtt
    58321 ctggaggaca catggcaaca cgaattgaat ttcacaagca cggtggcccg gaagtacttc
    58381 aagccgtaga gttcactcct gccgatccgg cggagaatga aatccaggtc gaaaataaag
    58441 ccatcggcat caattttatc gacacgtata tccgcagcgg cctttacccg ccgccatcgc
    58501 tacccagcgg attaggcacc gaagcggcag gcatcgtgag taaagtcggc agtggtgtaa
    58561 agcatattaa ggcaggcgat cgcgtagtct atgcacaatc ggcattaggc gcttacagct
    58621 ctgtgcataa cattaatgcg gataaagcgg cgattctgcc tgcggcaatt tcttttgagc
    58681 aagctgcggc atccttcctg aaaggcttaa cggtttatta tctgctgcgc aaaacctatg
    58741 aaattaaacc cgatgagcag ttcctgttcc acgcagcggc tggcggcgtt ggcttgattg
    58801 cctgccagtg ggcaaaagcc cttggcgcga aacttatcgg caccgtagga accgcgcaaa
    58861 aagcgcagag tgcgctaaaa gcgggcgcat ggcaggttat taactatcgt gaagagaatc
    58921 tggtcgagcg gttaaaagag atcaccggtg gcaagaaagt gcgcgtggtg tacgattccg
    58981 tgggcagaga cacctgggaa cggtcgctgg attgcctgca acgccgcggc ttaatggtga
    59041 gctttggcaa ctcatcaggt gcggttaccg gtgtgaactt aggcattctc aatcagaaag
    59101 gctcgttgta tgtgacacgc ccttccctac aaggctatat caccacacgg gaggaattaa
    59161 ccgaggccag taatgaactg ttctctttga ttgccagcgg tgtgattaag gtcgatgtcg
    59221 ccgagcagca gaaatatccg cttaaggatg cacagcgtgc gcatgagatt ctggaaagcc
    59281 gggcgacgca aggttccagc ctgttgattc cataaaagaa atagggcttc catctgggaa
    59341 gccctttctt tttatagttc ggctgtatgt agggtacagc acgatgaatc tgttagaggc
    59401 gcaatagtga cagatttgat tatcaattcc tattttgttc taaggataaa accttaggtt
    59461 gtgatcatcc gcacaatccc ttagtaacgc cagcggtcat aacgctgata tttcggcact
    59521 tttggtgctt taatcgcctt aataacccac accaccgcaa tcgccaggag taaccacggc
    59581 agcaacttaa tcatcaatgc cagcataccg ccgaggaaca taatggccgt cgccacaacc
    59641 agcgcggcga taatgcccag caacgaaacg ccggtgacca tcagcatgac aaaaaagcca
    59701 atcacaaaaa gtagttccag catgatgctc tcccaaatat gaaatctctt gcaggcatta
    59761 caagaatcat gccaaaaata atctattgat ttaacagcaa aacgccccgc gacggtgcgc
    59821 agggcgtggt gaatttgact actttttggt gaaaagttaa cgcttatccg ccaccagttt
    59881 gagcgcgtgt tccagcacgt taatgtcagc acccgcttta tgggcatttt cacttaaata
    59941 acgccgccac tgccgcgcgc caggaatacc ctggaacaag cccagcatat gccgggtaat
    60001 atggccgaga tacgtcccct ggctgagttc acgctcaatg tacggataca tggcacgcac
    60061 caccgccacc ggatcgacat cggtatccga agaaccaaag atctctcggt ctaccgccgc
    60121 cagaatgcct ggattctgat acgcttcgcg cccgaccatc acgccatcca tatgttgcaa
    60181 atgcgcttta gcctcttcca gcgacttgat gccaccgtta atcgacatcg tcagatgcgg
    60241 aaagtcacgc ttcagttgat acacacgcgg ataatcgagc ggcgggattt cacggttttc
    60301 tttcgggctt aaccccgaaa gccaggcttt acgtgcgtgg atgatgaaca tctcacactc
    60361 acctttgccg gaaacggtgt tgatgaaatc gcagagaaat tcatagctgt cctgatcatc
    60421 gatgccaata cgcgttttca ccgtcaccgg aatcgacacc acatcgcgca tcgctttcac
    60481 gcagtcggca accagctgcg cattacccat cagacacgca ccaaacatgc cgttctgcac
    60541 ccggtcagac gggcagccga cattcaggtt gatctcatca tatccgcgcg cttctgccag
    60601 cttcgcacac tgtgccagcg ccgccggatc gctacccccg agttgcaacg ctaccggatg
    60661 ttcttcttca ctgtacgcca ggtaatcacc tttaccgtga ataatcgccc ctgtagtcac
    60721 catttcggta tacagcaacg tattgcggga aagcagacgc aagaaatagc ggcaatgtct
    60781 gtccgtccag tcaagcatcg gggcaatcga gaaacggtaa gcgggataaa cagtgtgtga
    60841 ttctgctggc atggcgactt tttaagcatc tggtaaattg ggggcgctac tatagcataa
    60901 cgaatatgcg atcaggcaat gtgacagcca tgacaccctg ttctcggcgt ttaagcgaga
    60961 actatggtaa agtaaggaca ttcttaaccc cactgtcgag gtgcccaatg gaaaagacca
    61021 caacgcagga gttattagcg caggctgaaa aaatctgcgc acagcgtaat gtgcgcctga
    61081 ccccacagcg cctggaagta ttgcgcctga tgagtctgca ggatggcgct atcagcgctt
    61141 atgatctgct tgatttgctg cgcgaagctg aaccgcaagc caagccgcca acggtttatc
    61201 gcgcgctgga gtttctgctt gaacaaggtt ttgtgcataa ggtggaatcc accaacagtt
    61261 atgtgctctg tcatctgttc gatcagccca cccatacttc agccatgttt atttgcgatc
    61321 gctgcggcgc ggtgaaagaa gaatgtgctg aaggcgtgga agacatcatg catacgctgg
    61381 cggcaaaaat gggttttgcc ctgcggcata atgttattga agcacatgga ttatgttcag
    61441 catgtgtaga agtagaagcg tgtcgtcatc ccgaacagtg ccatcatgac cactctattc
    61501 aggtgaaaaa gaaacctcgc taaaagggtg tacatccttg tacatgtcgg gcaggaggga
    61561 ttaattacca gcggtaatca tggcgttttt cccaactatc aacctctttt tctgcctgat
    61621 ctttctgata accataacgt tcctggattt taccgaccag ttgatcacgt ttaccttcaa
    61681 tgatcgtcat atcatcatcg gtcagtttgc cccattgctc tttcacttta cctttaaact
    61741 gtttccagtt accgccggct tcatctttat tcataatcaa gacctcatcg ttaggttgtg
    61801 aatgagagta cgttcacttt tcttctgaac gtgagattaa gtgtagtcag caatttggct
    61861 taggattatt tattcagaat ttttaaccgt cacgttgcgg caaaccaggt accgttgcgc
    61921 cagtgacgcc gccagatagc cgccagagaa agcccgcgca aagccagaaa gacggttaat
    61981 gccagccaca aaccatgatt ccccagccac ggcagcgtaa ggagcgtcag cgcaaaacct
    62041 gcggcggcca ccgccatact gttacgcatt tcggcggcgc gcgttgcacc tataaacatg
    62101 ccgtcgagca gataacacca gacgccgacc aacggcaaaa tcacctgcca gataagatag
    62161 cggtcagcca gctgctgaat ctgggttaac gacgtcagca acgcaatgat gtgttcccct
    62221 gccagcaaat aaaccaccga aaacagtaac gctacgatcc ccgactggcg gcacgctgcc
    62281 cgccagacat ccagtaactg gctaccatcg cgcgcaccgt acgcctgacc ggaatgcgct
    62341 tcaaccgcgt aggcaaaacc atccagcgca taggcggtaa aggtgagtaa cgtcatcaga
    62401 accgcgttaa cagcgataat gtcactcccc agtcgcgcgc caagtacggt gatcgcgccg
    62461 aaacagagtt gcaacaacag cgagcgcagc atgatatcgc ggttaagcgc cagcaagtga
    62521 cggaagtttc ctcgccaggc agttttcagc atttcgccgg agattccgcg tagtttgagg
    62581 attttacgca ccattagcag accaatcagc aatgttgcat attccgcaat aaccgtcgcc
    62641 agcgccgcgc cctgcacgtt catatgcagc cccatcacca gccagacatc cagcacaatg
    62701 ttgaggatat tgccgaccac taacaaaatt actggcgcac gggcatattg cacaccgagt
    62761 aaccaaccaa gtaataccag attcgccagc gacgccggtg cacttaacca gcggatttca
    62821 agaaagcgtc gcgcctgttc tagcaccgct tcgctgccgc caacaatatg cagcgccaga
    62881 tcgataatcg gcgtacgcag cagcgcaatt aacgccccag cccccaacgc caacagcaac
    62941 ggttgcacca gcgcacgggc taatgcctga ggatttttgg caccataagc ctgcgcagtc
    63001 agcccggtgg tgctcatgcg taaaaacagc aacagcataa agagaaagct ggtcgccgtt
    63061 gcaccaaccg ccacgccgcc caaataaacc ggactatcaa gatgaccaat taccgccgta
    63121 tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa tcatgggtaa ggcgagatgc
    63181 cagagtgctt tatcagatga agtgaggaat gccatgcaga caagcctgat gaagagagat
    63241 gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag agatatgtta cagccagtcg
    63301 ccgttgcgaa taactccaac cgccagccct tcaatggtga agctctgctg acgaaggtca
    63361 acgacaattg gtttaaactc gctattttct ggcaacagtt cgactttatt gccctgtttt
    63421 ttcaggcgct taacggtaac ctcgtcatca atacgtgcga caacgacctg accgttacgt
    63481 acatcctgag ttttatgcac tgccagcaag tcgccatcca taatgccgat atctttcatc
    63541 gacatcccgc tgacgcgcag caggaaatca gcattcggct tgaataagga aggatcgacc
    63601 tgataatgac cttcaatatg ctgttgcgcc agaagcggtt caccggcagc cacacgacct
    63661 accagcggca acccttcttc ctcttcctgc aacagacgaa tcccgcgtga tgcgccggaa
    63721 acaatttcaa taacgccttt gcgtgccagc gccttcagat gttcttcagc cgcgtttggg
    63781 gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg
    63841 atgtgatcac ggatgagatc aaacacctct tgttgcctgg ccgttaacgc tttcattccg
    63901 ccccctgggt gtatatacag ttatgctgtg agtatataca gcaaaaggcg attttggaac
    63961 cataaactgc acaataaacc agagatttat cgaaatctgg aagggttatc caaaatgcga
    64021 ccataacagg atgcaccagg taatcacggc gacgataatc gcaatcagaa ccgcggcgga
    64081 tcccatatct ttagcgcgtc cggaaagctc atggtattca gagccaattc ggtcaaccac
    64141 tgcttcgatg gcgctattga ggatttccac aatcatcacc agcatcacgg agctgataag
    64201 cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg atgaccaccg ccaacaatac
    64261 cgctacgcct tcctgacgga atgccgcttc gttgatccat gcagcgcgta aacctttcca
    64321 ggaatagcca gcagctttga taattcgggt gaatccagtg gtattattgg ccattgaaag
    64381 aaccttttta cattatgagc gtcaatatca gtgtaccgtt aaatccgatg attcgggttt
    64441 gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt tcccatgact ttctgctatc
    64501 cttgccgcgc atttgcatta ttaaccagag gctttacatc gtttatgtcc ggctggccac
    64561 gaatttacta caaattactg aatttaccat taagcatcct ggtaaaaagc aagtctattc
    64621 cggcagatcc tgccccggaa ctggggctgg atacctctcg tccaattatg tacgttttac
    64681 cgtacaactc gaaagctgac ttgctgacgt tgcgcgccca gtgtctggca catgacctgc
    64741 ctgacccgtt agagccgctg gaaatcgacg gcacgctact gccgcgctat gtgttcattc
    64801 acggcgggcc gcgtgtgttc acctattaca cgccgaaaga agagtctatt aagctgttcc
    64861 acgactatct cgatttgcac cgtagcaacc caaatctgga tgtgcagatg gtgccagtat
    64921 cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga agtgaacccg ccgctgcgta
    64981 tgcttaacgg cgtacagaaa tttttcgctg tactgtggct cggtcgcgac agttttgtgc
    65041 gtttctcgcc gtcagtttcg ctgcgccgta tggcggatga acacggcacg gataaaacta
    65101 tcgcgcagaa actggcgcgc gtggcgcgta tgcactttgc ccgtcaacgt ctggctgccg
    65161 taggcccacg tctgcctgct cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca
    65221 ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc
    65281 agaacgcgat tgcgctgatg gaagagattg cggcgaattt ctcttacgag atgatccgcc
    65341 tgactgaccg tattctgggc ttcacctgga accgccttta ccagggcatc aacgtccata
    65401 acgccgagcg cgttcgtcag ctggcccacg acggtcatga gctggtgtat gtgccttgcc
    65461 accgcagcca catggactac ctgctgcttt cttacgtgct gtatcaccag gggctggtgc
    65521 cgccgcatat cgccgccggg atcaacctga atttctggcc tgccgggccg attttccgcc
    65581 gtctgggggc gttctttatt cgccgtacgt ttaaaggcaa taagctctat tccaccgttt
    65641 tccgtgagta tctcggcgaa ctgttcagcc gtggttattc cgtcgagtat ttcgtggaag
    65701 gcggtcgttc ccgtacaggg cgtttgctgg atccgaaaac cggtacgctg tcgatgacca
    65761 ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct gattccgatc tatatcggtt
    65821 atgagcacgt catggaagtg ggtacttacg ccaaagaact gcgcggtgcg acgaaagaga
    65881 aagagagcct gccgcagatg ctgcgcggtt taagcaagct gcgtaatctc ggtcagggtt
    65941 acgtcaactt cggtgaacca atgccgttga tgacctacct taaccagcat gtacctgact
    66001 ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc atggttaacg ccgacggtca
    66061 ataatattgc tgccgatctg atggtacgca ttaacaacgc aggcgcggca aacgccatga
    66121 acctgtgttg tactgcgctt ctggcgtcac gtcagcgctc actcacccgc gagcagttaa
    66181 ccgagcaact caactgctac ctggatctga tgcgcaacgt gccttactcc acggactcta
    66241 ccgttccttc agccagcgcc agcgagctta tcgatcacgc gctgcaaatg aacaagtttg
    66301 aagtcgagaa agacacgatc ggcgacatca tcattctgcc gcgcgagcaa gcggtgctga
    66361 tgacctacta tcgcaacaac attgcgcata tgttggtgct gccttcgctg atggcggcaa
    66421 ttgtcaccca gcatcgccac atctcccgcg acgtattgat ggagcacgtc aatgtgcttt
    66481 acccaatgct gaaagcggag ctgttcctgc gctgggatcg cgacgagttg ccgggcgtta
    66541 ttgatgcact ggcaaatgag atgcaacgtc aggggctgat taccctgcaa gatgatgagt
    66601 tgcatatcaa cccggcgcat tctcgcacgc tacagctgct ggccgcaggc gcgcgcgaaa
    66661 cgctgcaacg ttatgccatc accttctggc tgttgagcgc caacccgtcg atcaaccgcg
    66721 gtacgctgga gaaagagagc cgcaccgtcg cgcaacgtct ctccgtgctg cacggcatca
    66781 acgcgccgga gttcttcgac aaggcggtgt tcagttctct ggtgctgaca ctgcgtgatg
    66841 aagggtatat cagcgatagc ggcgacgccg aaccggcaga aacgatgaag gtgtatcagt
    66901 tgctggcgga gttgattaca tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg
    66961 aagggtaatc agggagaatt gccggatgcg gcgcaaacgc cttattcggc ctaccatgat
    67021 ctgcaaattc aataaattgc gatatgttgt gtaggcctga gaagcgcagc gcatcaggca
    67081 acccagaagg aagccggatg atcatccggc ttttttacat catcagaaat gccagtaact
    67141 cattgccagc cctaaaaaca gtaccagacc aacatagtta ttattcataa atgctttaaa
    67201 gcaggcttca cgctcgcggt tggcaatcag tttttgttga taaacaaaca gcgcgccagc
    67261 caccagaatt gaccagtaat atccccagcc taagccattt aactcaccga taatcgccat
    67321 cagcgccagt acgccaatct gcaaaatacc aataatcaat ttatcgtatt ggccgaacag
    67381 gattgcggtg gatttaatgc caatcttcac atcatcatca cggtcaacca tcgcatactg
    67441 cgtgtcgtat gccaccgccc agagaatatt ggcgaggaac attaaccagc aactcaatgg
    67501 caccgactca ctcacagcag caaaagccat tggaatcgac cagccaaacg ccgcgcccag
    67561 caccacttgc ggtagatggg tataccgctt cataaacggg tatacccacg ccagcgccag
    67621 cgcggcaatc gacaacagaa tagtcatcgt attcagcgtc agcaccagta aaaatgaaat
    67681 cagtaccagc actacaaaca gcgcgcgcgc ctctttctcc gttaccgcgc cgctggggag
    67741 tggtcggttc gccgtgcgct taacatgacc atcaaacttg cggtcagcat aatcattcac
    67801 cacgcaacca gcagcgcgca tcagccagac gcccgcgaca aacaccgcca ggatccagag
    67861 ctggggaacg cccggtgtcg ccacccacaa cgcccataat gttggccaga gcagcagtaa
    67921 cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac gccagcagct tattctgcgt
    67981 cagactccac tccatatttt tttcctctta gtacaacggt gacgccggta aaaacagttc
    68041 tgttagcaac agcggtttac cgcttaatcg caggcgggaa cgtcgccccc acagtccggc
    68101 atcacggcct atctcaataa agtcccgggt taatgtcgat gatgtgaaca gatagcgtcc
    68161 taacggcgtt ttacctaatt tttgtaacgc cagctccggc ccgcttaacg ttgacacagg
    68221 aacgacggta cgaccggcaa gccacggttc accatcggca cataacaaaa tttcacgtaa
    68281 ccagtaacga gactctttcg gcagcagtgg cagttcttcg gggatttcat tctgctcgac
    68341 aaacccttcg cggatcatcg tcacgctcac cgtttttccc tgctgttcaa aacgttttgt
    68401 catggaatct tccagcaaca gccagtcgag cagttgcggc tccagggcag ggatctctgt
    68461 aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt gacatgccga actctccgtt
    68521 acataacgta aaggcattgt atcgcagaaa aatatttacg ggagatgtaa agcaaattaa
    68581 gtgaactaat gcgcaacaaa aacgcaacaa tagtacagga ggagaaaaaa aggtgcgcca
    68641 ggagacgcac cagttgttgc aaaatcagca ctgtggggcg taattacccc ttgcctttta
    68701 cactgctgat aaaggtggaa cgggcagatg tcgaccccag gcgttcagct tcatcaagca
    68761 gttttaacgc cttatcaaca tcacctttcg cgacagcgtt tttgatcgcg gtattaaaat
    68821 aactttccgt gtcgttgagc atcggctcgc ttttcttcac cggtgccgga gcgggtgcag
    68881 ccacagctgg tgccgccgtg ttgcctaccg taaccggagc tggagcggaa gaaccaaata
    68941 gaggcccgac caacacgcta gaactggagt tcgttttgac tttcagtttc agtaagccat
    69001 cggtggtatg acgagcaacc gggtcaggaa tatccgggat cgagttaccg acgcctttgg
    69061 cataggcttt agccgggtcg agcagttggg tcgtctgctg gaggtctttt tccgtggtaa
    69121 agaccagaac ataaagtttt tgctgcccca acgccggtgt caggcgcata acgccttcca
    69181 gacgatctgc actcatcacg cctggttcct ggtaggtgaa ataactgctg gggaagaagg
    69241 ctgatggggt catgttctga tcaagaatca gcacgttcgg cgcaaatacg ctagtttgtt
    69301 tgttcacttc gctggtcagc gttagggtca gttcgccaat gtttgccggg acgctgtacg
    69361 cagcaaccgg acctctgatg ccgggaacgt tcagttgttg gccgccagtc gccagttggg
    69421 tggtctgggt tttagattga tcgaccggtg tccaggtgag ttgttgcagc gcggcagatg
    69481 gaatggctgg cgcgtcgctg gtgttttgcg gtacgtagtt aacatcggca aggctgattc
    69541 caggtgcgct tgccagcaac cctgctgata aacagagagc gatgagactt ttattcattt
    69601 tcattgtttt cacctcaaaa tctggagctc agcggtagcc aggcaatagc gcgctaaacc
    69661 cgataatcag aggggctttc gccccttaaa gctgttttta tgccggatgc ggcgtaaacg
    69721 ccttatccgg cctactttcg gcagcggttg tagttgtagg cctgataaga cgcgcaagcg
    69781 tcgcatcagg cgttggttgc cggatgcggc gcaagcacct tatccggccc aggttttgct
    69841 attaccacca gatttccatc tgggcaccga aggtccactc gtcgctgtca ccacggccga
    69901 agctgccgcc gttgaaatca gcaggaacgg ctttgccgaa gttagtgttg gtattggcgt
    69961 taccggtgta gtcataaccc catttctcat cccacttggc gtaggttgcg aagacacgaa
    70021 tagccgggcg tgaccagatg ctgtcgccag cctgccattg ttgtgcaagg gtaattttgt
    70081 actgattgtt cttgtcgccg gtgcgctggg attcgacgtt gtcgtagccg atttccatca
    70141 cggtgctcat gattggcgtc cacttgtaca tcgggcgaat accgacggtc caccacttgg
    70201 tgccgttgtc gttatcccag ttgatatcct ggtacatacc cacgtacatc atgtcccagt
    70261 tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag catgtgaccg ttgttgttga
    70321 tattgtaggc aaatttttcg ttatcaaacg cgacgccaga accctgcgac agacctttac
    70381 cctgcgaggt catcgagtca gtagcgtact gaacaacaaa cttgttaaag cccttcagga
    70441 cactctgagt atgttcagca gtgaataacc aaccgtcttt cgatgcgcca tcaaccagac
    70501 gatagttatc acgcaggttg gcacgaccgt agtcgacacc cagttctaat gtgccgcccg
    70561 ggttgatttc catctgcgct aaacgcacgt cgaaaacgtc gttcgcggtt tcgttggtat
    70621 agtcataaat attgttgctg gcgaaagagg aagaaccacc agcttcagag gaacgggttg
    70681 ctgccagaga gagtttaccg aagccaacat cgatgttttc cagaccggca ccaggaccag
    70741 aaatatccca gtagtagaag tcgatcatat gaacgtcatg acgttggtag aagcgcttac
    70801 ctgcccagat ggtggaacct ggcagccatt cgatcaggtt tttaccctgc acgtttgctt
    70861 cacggaaggc cggatcggta gcttcccagt cattctgttg tgcgacggaa taggccacgt
    70921 tagtgtcgaa atagaagctc ttatcgccct ctttccacac ttcctgaccc aattttaatt
    70981 cagcataagt ttcacattcg ttgccaagac ggtatttact ttgagcaccg gtagtctgga
    71041 aacactgttg ttcaccgccg ctacctgtcc agccaatacc ggaacgtgca tagccgtgga
    71101 aatcaacagc cattgcctga gcagacatta cgcccgctgc gacggcaacc gccagaggaa
    71161 gtttgcgcag agtaatcatc attctatctc ctgagtcatt gcttttcttt tttcacatca
    71221 cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg cccggctcct tatgcagtcg
    71281 acgacatgca gtgccatcct cacggaacag atggcaacgc tctggcggca ggccgatagc
    71341 gaatgtggca ccttcttcta ccaacaccac gtcgttctgg cggtacacca ggttttgacg
    71401 aatggaaggg atctggatat ggatttgggt ttcgttgccg agttgctcga cgacctgaac
    71461 ttcaccctca aggatgacgt cagcgatatc actcggcagt agatgttccg ggcgaatacc
    71521 cagcgacata ttggctccaa cctggacatc acggctttca accggcagcc agacttgctg
    71581 acgatttggc atcggcagct ccacctgcac ttgatcgatt gcagtggcgg tcacttttac
    71641 tggcaggaag ttcatctttg gcgaaccgat aaatccggcg acaaaacggt ctgccggata
    71701 gtggtacagc tccagcggtt tcccaacctg cgccacgcga ccggcgtcca gcaccacgat
    71761 tttgtcggct agtgtcatcg cttcgacctg atcgtgggtg acgtaaatca ttgtgcggcc
    71821 caggcgttta tgcagacggg agatttcgat acgcatttgc acacgcagtg cagcatcgag
    71881 gttggagagc ggttcatcga gcaaaaatac gcttggctcg gctaccagcg tacggccaat
    71941 cgccacacgc tgacgctgac caccggagag cgctttcggt ttgcgatcca gcaaatgcgc
    72001 cagttgcagc acttccgcaa cctggttaac tcgctggtta atcacttctt ttttcgcgcc
    72061 agccagtttc aggccaaatg acatgttttc tgctactgac aggtggggat agagcgcgta
    72121 agactgaaac accataccaa cgccgcgttc tgcaggcgga gtgtcattca tccgtttctc
    72181 accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg gcaatcatgc gcagtaaagt
    72241 cgatttaccg cagccagacg gtccgacaaa caccacgaat tcaccttcat ggatatcgag
    72301 attgatatct ttcgatacca cgacctcgcc ccaggctttc gttacatttt gcagctgtac
    72361 gctcgccatg cccttctccc tttgtaacaa cctgtcatcg acagcaacat tcatgatggg
    72421 ctgactatgc gtcatcagga gatggcttaa atcctccacc ccctggcttt tttatggggg
    72481 aggaggcggg aggatgagaa cgcagcttct gtgaactaaa ccgaggtcat gtaaggaatt
    72541 tcgtgatgtt gcttgcaaaa atcatggcga ttttatgtgc gcatctccac attaccgcca
    72601 attctgtaac agagatcaca caaagcaacg gtggggcgta ggggcaagga ggatggaaag
    72661 aggttgccgt ataaagaaac tagagtccgt ttaggtgttt tcacgagcac ttcaccaaca
    72721 aggaccatag attatgaaaa taaaaacagg tgcacgcatc ctcgcattat ccgcattaac
    72781 gacgatgatg ttttccgcct cggctctcgc caaaatcgaa gaaggtaaac tggtaatctg
    72841 gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga
    72901 taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tccctcaggt
    72961 tgcggcaact ggcgatggcc ctgacattat cttctgggcg cacgaccgct ttggcggcta
    73021 cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta
    73081 tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt
    73141 cgaagcgtta tcactgattt ataacaaaga tctgctaccg aacccgccaa aaacctggga
    73201 agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa
    73261 cctgcaagaa ccatacttca cctggccgct aattgcggct gacgggggtt atgcgttcaa
    73321 gtatgaaaac ggcaagtacg acattaaaga cgtaggcgtg gataacgctg gcgcgaaagc
    73381 gggtctaacc ttcctggttg atctgattaa aaacaaacac atgaatgcag acaccgatta
    73441 ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgactatca acggcccgtg
    73501 ggcatggtcc aacatcgaca ccagtaaagt gaattatggt gtaacggtac tgccgacctt
    73561 caaaggtcaa ccatctaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag
    73621 tccgaacaaa gagctggcga aagagttcct cgaaaactat ctgctgactg acgaaggtct
    73681 ggaagcggtc aataaagaca aaccgctggg tgcagtagcg ctgaagtcct atcaggatga
    73741 acttgcgaaa gatccacgta ttgccgccac catggataac gctcagaaag gtgaaattat
    73801 gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc
    73861 cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagactc gtatcaccaa
    73921 gtaatgctgt gaaatgccgg atgcggcgta aacgccttat ccggcctacg aaattgaaac
    73981 gtatgtaggc ctgataagac gcgtcagcgt cgcatcaggc agttgttgtc ggataaggcg
    74041 tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg gatgtcatta aaaagaaaca
    74101 ttggtggcaa agcgacgcgc tgaaatggtc agtgttaggt ctgctcggcc tgctggtggg
    74161 ttaccttgtt gttttaatgt acgcacaagg ggaatacctg ttcgccatta ccacgctgat
    74221 attgagttca gcggggctgt atattttcgc caatcgtaaa gcctacgcct ggcgctatgt
    74281 ttacccggga atggctggaa tgggattatt cgtcctcttc cctctggtct gcaccatcgc
    74341 cattgccttc accaactaca gcagcactaa ccagctgact tttgaacgtg cacaggaagt
    74401 gttgttagat cgctcctggc aagcaggcaa aacctataac tttggtcttt acccggcggg
    74461 cgatgagtgg caactggcgc tcagcgacgg cgaaaccggc aaaaattacc tttccgacgc
    74521 ttttaaattt ggcggcgagc aaaaactgca actgaaagaa acgaccgccc agcccgaagg
    74581 cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag gcgctgagtg acattaccgc
    74641 cattctgccg gatggcaaca aagtgatgat gagctccctg cgccagtttt ctggcacgca
    74701 gccgctctac acactcgacg gtgacggcac gttgacgaat aatcagagcg gcgtgaaata
    74761 tcgtccgaat aaccaaattg gcttttacca gtccattacc gccgacggca actggggcga
    74821 tgaaaagcta agccccggtt acaccgtgac cactggctgg aaaaacttta cccgcgtctt
    74881 caccgacgaa ggcattcaga aaccgttcct cgccattttc gtctggaccg tggtgttctc
    74941 gctgatcact gtctttttaa cggtggcggt cggcatggtt ctggcgtgtc tggtgcagtg
    75001 ggaagcgttg cgcggcaaag cggtctatcg cgtcctgctg attctgccct acgcggtgcc
    75061 atcgttcatt tcaatcttga ttttcaaagg gttgtttaac cagagcttcg gtgagatcaa
    75121 catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg ttcagcgatc cgaccaccgc
    75181 ccgaacgatg ctgattatcg tcaatacctg gctgggttat ccgtacatga tgatcctctg
    75241 catgggcttg ctgaaagcga ttccggacga tttgtatgaa gcctcagcaa tggatggcgc
    75301 aggtccgttc cagaacttct ttaagattac gctgccgctg ctgattaaac cgctgacgcc
    75361 gctgatgatc gccagcttcg cctttaactt taacaacttc gtgctgattc aactgttaac
    75421 caacggcggc ccggatcgtc ttggcacgac cacgccagcc ggttataccg acctgcttgt
    75481 taactacacc taccgcatcg cttttgaagg cggcgggggt caggacttcg gtctggcggc
    75541 agcaattgcc acgctgatct tcctgctggt gggtgcgctg gcgatagtga acctgaaagc
    75601 cacgcgaatg aagtttgatt aagggagata acaaaaatgg caatggtcca accgaaatcg
    75661 caaaaaacac gtttgtttat tactcacctg ctactgctac tttttatcgc ggcgattatg
    75721 ttcccgctac tgatggtcgt cgctatctca ctgcgccagg ggaactttgc gaccggcagc
    75781 ctgatcccgg agcaaatctc ctgggatcac tggaaactgg cattaggttt tagcgtggaa
    75841 caggctgatg gtcgcgttac accgccgccg tttccggtac tgctatggct gtggaactcg
    75901 gtaaaggtcg ccgggatttc cgcgattggc attgtggcgc tctccaccac ctgcgcctac
    75961 gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc tgaaaggaat gctgattttc
    76021 cagatgttcc cggcagtact ttcactggtc gcgttgtatg cgttgtttga tcgtctgggt
    76081 gagtacattc cattcattgg cctgaatact cacggcggcg taattttcgc gtatctgggc
    76141 gggattgcgc tgcatgtctg gaccatcaaa ggttatttcg aaaccatcga cagttcgctg
    76201 gaagaagcgg cggcgctgga tggtgcgaca ccgtggcagg ccttccgcct tgtcctgttg
    76261 ccgctgtcag taccgattct ggcggttgtg ttcatcctgt cgtttatcgc tgccattact
    76321 gaagttccgg tcgcgtcgct attactgcgt gacgtaaaca gctacaccct ggctgtgggg
    76381 atgcagcaat acctcaaccc gcaaaactac ctgtggggtg actttgccgc cgctgccgtg
    76441 atgtctgcat taccgatcac catcgtcttc ttgctggctc aacgctggct ggtcaacggc
    76501 ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg ccaatgttat gccgctgcac
    76561 cctcaactta cgttatccca acttgtgact gttattcggc gctccacgga gcgccttttt
    76621 ttactcccgc ttcagccgtt tcgaattaca caaccacagg gtgatcacca gtaacaggat
    76681 cgctgccgag tagatcaaca catccagtgg cgatttatga tcgacgatga tcaagcgcac
    76741 aatggcggtg atcccaatat agacaaagta acgtagcggg aagtgaaaac cggactgaaa
    76801 gtacttcaca atcagcgcga taaattcgaa atagagaaag taaaccacca gcccttctac
    76861 cagctcatat ttactggttt gttctggcgc gaacagcaca tcagccagat gcaccgtttc
    76921 tttgccgagg aagacaacca aaatcaggcc gaggcacaac aggccaagat tgagtacggt
    76981 ctgcaaaatg gtggatataa actccacgcg cggacgagag agtgacgtca tagcagtacc
    77041 tccttcacag tggcgagctt cctgtataac gcaaaaatgt gacggagatc tatatttttt
    77101 gtgtatgttt tgttcagctc aattaagtgg gcatctccca tcaatgtctc atctggcaaa
    77161 acacacagcc aaaaaaatca cccattcagg tgattcccag ccaccagcaa tgcgcgaaaa
    77221 atcgacaatg ttcctctgga acaaatgaaa cgtaacctga taaaggtggt aaaaatgaag
    77281 ccttattttg ctgctttgat gttatcagtc tctgttcttc ctgcttatgc tggtccgctc
    77341 ggtactgctg ataaagccga cctaccgcaa agcaatgtct ccagtccaat gatggcccag
    77401 tcgctcaggc aaccagacct gcaaccgata tcgacggaca ggaagacaga atgttttcgc
    77461 ctttacacac cagatcgcaa accgggggtg aactgcgttc ctgatggctc gacaggccat
    77521 taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt ttccatgact tagcggaaat
    77581 tgacgctacg gtcgctggtc atgtcataca actggaactt acgcccaagt tgctgaccac
    77641 cgtcacgcgt cagcggcgtc cagccaattg ccgcacggct acgtgtcggg ccagacgaga
    77701 agagatccag cggtacagag acatacacgc ctttggtaaa gtccccttcg ccgtactcct
    77761 ctttcgaaac gttggtgatc gtggcatagc cacccaccac cacgccgcta tcaaagcgtt
    77821 tggcgatctc cagcgtgccg cctttatccc ctgccagata ctgcccgacg ctcgctttca
    77881 ctaacacatc ctgagcaaaa gatggcgtcc agtatgcggt cagatgtccg gttttcacgc
    77941 tgtagtcggt gaatttcatc atatcttttg cactacgcca gtcgcgctgt ttaacgtagt
    78001 tggcatccag accaaacgcc cagttgctgt ccagcgggcg atacaacact tctgcccccg
    78061 caccgccaaa catggtttcg agataacctc cgtagacctg accgtagaag ccgttgccca
    78121 gatgctggaa gtagttggct tgcaggttat tcacatagac atcgttctgc acatactcgc
    78181 gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg cggattggtg tagttaaact
    78241 tgtcgtagtt gttggcaagg ttagcaaaca ggctgccggt ggtcagcaga tggtccgtca
    78301 gccacaaatc cgccgttccc atcacgccca gctgatacat gtaaaagttt tccgggccac
    78361 cgaccgactg gttcaacacc ggatcgatat ggaaatcaaa gcgtgatttg tcgatatacc
    78421 agccctgctc ggtggactgc ggaaccactg gctcgacgcg tttttgcgcc agcttcgttt
    78481 cgtggcccaa cggctctccg gcgagatgat ttttcaggct ggcgacatcg gtttccgtcg
    78541 tcacctgcgg catgttaagg cgattttccg taatgcggat cgttttgatc ccatccggca
    78601 gatcgttcat cacgatccga ttagcacgga tgatcccttc acgcgaatca cgatatttca
    78661 cctgctcgcc ggtgacatac agcgtatcgc ctttcgtctg gatctgcgga tcggccagtc
    78721 cggcgttgta tttcaacagc gttaactgat tcgccaccac cgaatgctgc aaaatggcat
    78781 cctgtggttg cggttgatat tgcgggcggg cgttatcgtt gtaggacggg cgcagatcgt
    78841 taaagttggt gcgcagcgtg acgccaaaca taaaagtgtt gccacgttca tagctaaggt
    78901 taacgtcggc ccaatcggta acgcgataaa tcgccccgac gttaaactta cttttttgct
    78961 ccagcttacc ggcaaaatcc tgctgataat tattgccttc atactccagt ttcaggcgca
    79021 gcggttgcca gggcgtctgg tattccacgc cgccaaacag tgaggccgga ccgtggaaca
    79081 tctggctgcc gtcgatagat cccgcttgtt tgtagctgtt atcgcgatag caatatttgt
    79141 cactggccga acagagcgga tttttcacat taccgctggt gcccagatat ccccagccca
    79201 ggccgagcgt aaaatcgaat ggcccccagg ctttgctggc gacaagatat tccgcatcga
    79261 acagccccgt accgccgata tcccgcgcac caaccgccac ttgcggcagc cagtaactct
    79321 cttcccacaa acgcagtttg agatcgaagg ctttatcttt atacgtttga tcgccagaga
    79381 acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt gtagcgcagc gttgtttcca
    79441 gccacgggaa gagttgcact gaagctgagt aataacggta ctgatcgtta tcgcgatagt
    79501 tcagactcag ctccccttcc cgcgccatgc gcgcggtggg cgtttgtaat aatcctacgc
    79561 caccgaaatc cgattgcgac ggaccaatgg gcgcaggata tgtttcgccg tagcaagctg
    79621 tgctaatacc cagcgccagt aagctaagca gatgtctttt tttcattatt ggggtatccg
    79681 ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca ggtgtctcac tccagacgga
    79741 gtccgcgagg ccaacataaa taatgctgcc gggcatcggc tctacgtgac gcttgttcca
    79801 gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa accacccacg cgtagctgcg
    79861 atccgcaccg ctaagcaggt tctgcccgga gagatagctc gccacgtcgc gacctggagt
    79921 gaatggctgc ttgccaggac ggctgataag cccgaacaac gtgacagtgg acggtggtgg
    79981 tccgacccac agtgtgtagt tgccctgcaa cggtgggtta ccacgttcgg caacgcgcac
    80041 gatatcggga tcaagattga ttttttgcct gcccgtcact ttcaacgcct gaatttgctg
    80101 gcgtaaggcg ttaatcgcag cggcatcgtc agcgctggaa tctgcggcca gttctgccag
    80161 ccgtgccagt agcgcctgct gctgccgcaa tgctgccgcc gttgccagct cttcgctgat
    80221 cactgcacca ggccaccagc tgtttgccag tcgaggttgc cctaccagat cgattaaatg
    80281 ctctgcgccc gttagcgttt tggcctcact gctgccattg ctgaacacct tgacggttcc
    80341 tgccgcgaag accgatgacg cccccacact caaaatcaat gcgacaatag tttgtttaat
    80401 cattgttttg ccgccttgat gagagtggtt ttcaccggga aataatccgc gccgagatac
    80461 tgttccgact ggcgaatttg cccttcgctg tcgatccagt agcggttatg ccagcgcgcc
    80521 tggtcggtgg agacttcttc gtccagcacg cgaaccgggg tttcatcgct gccgaccttg
    80581 acggtatcgg tgccatccca tttgaagact gagcgtgcgg tggcgtagcg tacctgctgg
    80641 tactcgatcc agcccatcgt gcgcgtccat gttgcaccat caacaatttg tgcgggttta
    80701 atcagcgggt cggcggcaag gttattcact tcaatcaggt tgtcgccgcc aagcaaagtc
    80761 ttcaccagac gaccatgttg tgtgacgaga gtggcctgat cctgagtgac ccatttttgt
    80821 tgtccgtctt cagcgaaggc cagcaccaca aataactgcg gcccgccatt aagctgcatg
    80881 tactggctgg cgtagggcat attttgaata tcatcgtccg tcagctgtac gcctggcgtg
    80941 ccgaacagac tgtcccacag tgaattgccc agctctttag tcgtggctga acaggcctgt
    81001 aatagcaggc agataagaat gagtgcaggt cgcttcacga ctcttctcca gaggggcaga
    81061 ataaccacac cggagtgtgg ttatggttaa tccccttcat acttcaagct gcagatgcgg
    81121 tgaccgcact cgttcacccc ggtcacatag ttatctatgc tcctggggac tcactcgctt
    81181 gtcgccttcc tgcaactcga attattttgg ggatacatac tttattactg ggtactggtg
    81241 gtcgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccgcttgc cgcgagcgcg
    81301 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    81361 gacgttgctg ccgaccctgc cgcttcgccc acctgtacag gtgcagccca cgcagaagtc
    81421 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    81481 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    81541 aagcatgcga attacggcgc ggggaattag tgatggggtt ttaggataat tggaatgaat
    81601 atcaaatata agcttcagct tatatataaa tagcactaaa aatcataagc ttaaaataaa
    81661 aataaaagta attaatctgc gtattttccg aaatcaatca cgattaaaaa tctattttag
    81721 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaaa
    81781 atgccgacat aacgtcggca ttgtttttaa ggctatttaa tcaatcgtac tatgtattaa
    81841 ttgccgaata cgatgtacat atcggcatcg acctgtaggc ctgataagac gcgaccgcgt
    81901 cgcatcaggc atcggttgcc ggatgcggcg tgaacgcctt atccggccta catatcgaca
    81961 atgattaacc gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc
    82021 tgctgatttc tttatcatct ttcagctctg gcagaatacg gttcgccagc tgtttaccca
    82081 gctccacgcc ccactggtcg aaggtgaaga tgttcagaat cacgccctga gtaaagattt
    82141 tgtgctcata cagcgcaatc aatgcaccca ggctgaacgg agtgatttca cgcagcagga
    82201 tggagttggt cgggcggtta ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg
    82261 ccggatcttt accctgatca cgatattcct gctcaaccac ttcgcgggat ttaccaaacg
    82321 ccagcgcttc ggtctgagca aagaagttag acagcagttt ctggtgatga tcagagagcg
    82381 ggttatgggt gatagccgga gcgatgaaat cgcacggtac cattttggtt ccctggtgga
    82441 tcagctgata gaacgcgtgc tgaccgttcg tgccaggttc accccagata atcgggccag
    82501 tctggtaatc cacaacctta ccgttacggt caacatactt accgttggat tccatattgc
    82561 cctgctggaa gtacgccgcg aaacggtgca tatactggtc atacggcaga atcgcttcag
    82621 tttccgcgcc aaagaaattg ttgtaccaga tgccaatcag cgccagcaag acaggcaggt
    82681 ttttctcggc aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt
    82741 caacaaaatt atcaaagccg atggagagaa caatcgacag gccaattgct gaccacaaag
    82801 agtaacggcc gccaacccag tcccagaact cgaacatgtt ggcagtatca ataccaaact
    82861 cgccaacggc tttggcattg gtggaaagcg ccgcaaagtg tttcgcaacg tgtttttcat
    82921 caccagccgc tttcaggaac cagtcacgcg cgctgtgggc gttggtcatg gtttcctgag
    82981 tggtgaaggt tttagatgct accaggaaca gcgtggtttc cgggtttact tttttcagca
    83041 cttccgcgat gtgagtccca tcgacgttag aaacaaagtg catgttcagg tggtttttgt
    83101 atggacgcag tgcttcagtc accatgtacg ggccgaggtc agaaccgccg atcccgatgt
    83161 tcactacgtc agtgattgct ttgccggtat agcctttcca ctcaccggaa ataatcgctt
    83221 ccgagaaggt tttcatcttc tccagcaccg cgttgacttc tggcattacg tctttgccat
    83281 caaccagaat cggggtattg ctacggttac gcagcgctac gtgcagcacg gcgcggtttt
    83341 cagtgcggtt gatcttctcg ccagagaaca tcgacttaat cgcgcccgcc agatcgcact
    83401 ctttcgccag atcttgtaat ttcgccagcg tctcttcagt gatgcggttt ttggagtaat
    83461 ccaccagcat ctgatcgtcg aatgttgcgg agaacttaga aaaacgatcg ccgtctttag
    83521 caaaaagatc ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc
    83581 aggcagcggt ctgcgttgga ttgatgtttt tcattagcaa tactcttctg attttgagaa
    83641 ttgtgacttt ggaagattgt agcgccagtc acagaaaaat gtgatggttt tagtgccgtt
    83701 agcgtaatgt tgagtgtaaa cccttagcgc agcgaagcat ttattagctg aactactgac
    83761 cgccaggagt ggatgaaaaa tccgcatgac cccatcgttg acaacggccc cgctcaccct
    83821 ttatttataa atgtactacc tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa
    83881 cggtgttgga ggagccagtc ctgtgataac acctgagggg gtgcatcgcc gaggtgattg
    83941 aacggctggc cacgttcatc atcggctaca ggggctgaat cccctgggtt gtcaccagaa
    84001 gcgttcgcag tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa
    84061 gtatcgctct gcgcccaccc gtcttccgct cttcccttgt gccaaggctg aaaatggatc
    84121 ccctgacacg aggtagttat gtctgaaatt gttgtctcca aatttggcgg taccagcgta
    84181 gctgattttg acgccatgaa ccgcagcgct gatattgtgc tttctgatgc caacgtgcgt
    84241 ttagttgtcc tctcggcttc tgctggtatc actaatctgc tggtcgcttt agctgaagga
    84301 ctggaacctg gcgagcgatt cgaaaaactc gacgctattc gcaacatcca gtttgccatt
    84361 ctggaacgtc tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct gctggagaac
    84421 attactgttc tggcagaagc ggcggcgctg gcaacgtctc cggcgctgac agatgaactg
    84481 gtcagccacg gcgagctgat gtcgaccctg ctgtttgtcg agatcctgcg cgaacgcgat
    84541 gttcaggcac agtggtttga tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt
    84601 gcagagccag atgtagccgc gctggcggaa ctggccgcgc tgcagctgct cccacgcctc
    84661 aatgaaggct tagtgatcac ccagggattt atcggtagcg aaaataaagg tcgtacaacg
    84721 acgcttggcc gtggaggcag cgattatacg gcagccttgc tggcggaggc tttacacgca
    84781 tctcgtgttg atatctggac cgacgtcccg ggcatctaca ccaccgatcc acgcgtggtt
    84841 tccgcagcaa aacgcattga tgaaatcgcg tttgccgaag cggcagagat ggcaactttt
    84901 ggtgcaaaag tactgcatcc ggcaacgttg ctacccgcag tacgcagcga tatcccagtc
    84961 tttgtcggct ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa
    85021 aatccgccgc tgttccgcgc gctggcgctt cgtcgcaatc agactctgct cactttgcac
    85081 agcctgaata tgctgcattc tcgcggtttc ctcgcggaag ttttcggcat cctcgcgcgg
    85141 cataatattt cggtagactt aatcaccacg tcagaagtga gtgtggcatt aacccttgat
    85201 accaccggtt caacctccac tggcgatacg ttgctgacgc aatccctgct gatggagctt
    85261 tccgcactgt gccgggtgga agtggaagag ggtctggcac tggtcgcgtt gattggcaat
    85321 gacctgtcaa aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga accgttcaac
    85381 attcgcatga tttgttacgg cgcatccagc cataacctgt gcttcctggt gcccggcgaa
    85441 gatgccgagc aggtagtgca gaagctgcat tttaatttgt ttgagtaaac acagtatggc
    85501 ccggaagaca ttatttcggg ccgtattgtt tttcttgtca ttatgcccat aaataaacga
    85561 gcctgtactc tgttaaccag cgtctttatc ggagaataat tgcctttaat tttttatctc
    85621 tttctctaac taattagaga aagagataaa tagttaagag aaggcaaaat gaatattatc
    85681 agttctgctc gcaaacgaat tcccgtacac tgaactctct ggctcactga gggcaccatc
    85741 atggcactcc cccgtattac ccaaaaagag atgaatgaac gcgagcagcg tgaacttaaa
    85801 acactgctgg atcgggcgcg gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac
    85861 agcattaaga aagagtacat cgataaatta atggtcgagc gtgaggctga agcgaaaaaa
    85921 gcccgccaat tgaagaaaaa gcaggcttat aaaccggatc ctgaggcgtc atttagctgg
    85981 tcagcaaata cgtcgacgcg cggcaggcgc tgattaacgc cccttctttt tgcgccccgg
    86041 cgaggtaaaa cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg ttttttccat
    86101 cttaacgact ggacgtttga tgcctaccgt tttcggtttg gccttcgctt taggttttgc
    86161 ctcggaagag gaattttcga tgagcttaaa gaggtcgatt aactcatcgt cggttaaatc
    86221 gcgccactcc cccagcggaa tgccgcttaa actgacgttc ataatgcgcg tacgttccag
    86281 ctttttcact tcgtaaccaa agtgctcgca catgcgacgg atctgacggt tcagcccctg
    86341 caccagggta atgcggaaga caaatggcgc ttcttttttg actttgcact ttttagtcac
    86401 tgtcccgagg attggcaccc ccgcccccat accacgaata aattcatcgg taatcggttt
    86461 atcgaccgtc accagatact ctttctcatg atcgttaccc gcacggagga tcttattcac
    86521 cagatcgccg tgattggtga ggaaaatcag cccctgggag tctttatcca ggcggccaat
    86581 cgggaatacg cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc
    86641 ggtggtgctg acaataccga cgggcttgtt cagggcgata agtaccaaat cttcggcttc
    86701 ccgaggttca atcaactgac catttacttt cacaacgtcg ccgggtttca cctgatcgcc
    86761 aatggtggct cgcttgccat taaggaacac attgccttgc tcgatatagc gatccgcttc
    86821 gcggcgtgag caaattccgc tttcgctgat gtatttattt aaacggactg atgagtcggg
    86881 cagcataaat tctcctgtaa aacgcggaat ataccgtacc tcttagcggg gaaaaacagg
    86941 gttatgcggc aacgtcgaaa ttaaatgtga tctgccacgc cttttatcac aaaagtgtct
    87001 ccggaagtac gatgcaaatt tgcctggcaa aataaggata atcaacatat tacgaatcgc
    87061 tgcacacctg tgctgatgac tactacgcag agaaacaaat gtgcaagaga aagcgctatt
    87121 tctccccatg caaagagtga aaaatcgctt tgaaaggccc tcatgatggg gttatatcag
    87181 atcaagcggt gagaaagcgt atttgcacaa atgtgcagaa ccaggaggcg gcaatggaaa
    87241 acagtgacga tatccgtttg attgtgaaga ttgcccaact ctattacgaa caggatatga
    87301 cgcaggcgca aatcgcgcgc gaactgggta tttaccgcac caccatcagc cgcttgctta
    87361 aacgaggccg cgatcaggga attgtcacca tcgccatcaa ctatgactac aacgaaaatc
    87421 tctggctgga gcagcaactg aagcaaaagt ttggcctgaa agacgttgtg gtggtgtcag
    87481 gaaatgatga ggatgaagag actcaactgg cgatgatggg gttacacggc gcgcaactgc
    87541 tggatcgctt gctggaacct ggcgatattg tcggtttttc ctggggccgc gcggtgaacg
    87601 cactggttga aaacttgccg caggcggggc aatcgcggca gttaatctgc gtaccaatta
    87661 ttggcggacc gtccggtaaa ctcgaaagcc gctatcatgt aaacacatta acctacagcg
    87721 cggcagcgaa gttgaaaggg gaatcgcatc tcgcggattt tccggctctg ctggataacc
    87781 cattaattcg taatgggatc atgcagtctc agcactttaa aaccatctct gcctactggg
    87841 ataatctgga tgtcgccctg gtgggaattg gctcaccggc cattcgcgac ggcgctaact
    87901 ggcatgcgtt ttatggtggt gaagagagtg acgacctgaa tgcccgccag gttgctggcg
    87961 atatttgctc gcgctttttt gatattcacg gcgcaatggt tgaaacaaat atgagcgaaa
    88021 aaacactctc tatcgaaatg aataaattaa agcaggcgcg gtattccatt ggcattgcca
    88081 tgagtgaaga aaaatacagc ggaattattg gtgcactgcg tggaaaatat attaattgtc
    88141 tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc cgatgtttac
    88201 cgtaataaaa attacggcgg gtctctatta tctaaaatca ggagtctgtt atgcaaacgt
    88261 ggttaaattt gcaggataaa atcattattg tcaccggtgg cgcatccggc attggtctgg
    88321 cgattgtgga tgaattatta gcacaaggcg cgaatgtaca gatggtcgat attcacggtg
    88381 gcgatggaca atatgaaggt cataaaggct atcagttttg gccgaccgat atttccagcg
    88441 ccaaagaggt aaatcatacg gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc
    88501 tggtcaataa cgccggggtc aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg
    88561 ggcagtatga actcaacgaa gctgcattcg aaaaaatggt caatatcaac cagaaaggcg
    88621 tttttctgat gtcgcaggcg gtggcgcgac agatggtcaa acaacatgat ggcgtgattg
    88681 tgaatgtttc ctcagaaagt gggctggaag gctcagaagg ccaaagctgt tacgccgcta
    88741 ccaaagccgc gcttaatagc ttcacgcgat cctggtcgaa agaattgggt aaacacggta
    88801 tccgtgtagt cggtatcgcg ccggggattc tggaaaaaac caggctacgc acgccggaat
    88861 atgaagaggc gctggcgtgg acgcgcaata tcaccgtcga gcagctgcgt gaaggctata
    88921 ccaaaaacgc cattcctatt gggcgcgccg gaagattagc agaagtcact gattttgttt
    88981 gttatctgct gtctcaacgc gccagctata tcaccggagt aaccactaac attgcgggcg
    89041 gcaaaacgcg cggctaagga ggctttatgg tcaacgccat tttttgtgcc cacggcaaat
    89101 tagcttgcgc catgctggaa tcggttcaga tggtttacgg caacgccaat gttgaagccg
    89161 ttgaatttgt gcccggtgaa aacgccggag acatcgtagc aaagctggaa aagttagtaa
    89221 gcattcacaa tcaagatgag tggctgattg ccgtggattt acagtgcggc agcccgtgga
    89281 atgcggcagc gatgcttgcg atgcgcaatc ctcgtctgcg ggtaatcagc ggcctttctc
    89341 tgccgctggc gctcgaactg gttgataacc agcacagcat gaatgttgat gaactgtgtg
    89401 aacacctgac gcaaatcgca aagcaaacct gcgttgtctg gaaacaactg gcaacggcag
    89461 aggaggattt ctgatgaaca ttacgctcgc ccgcattgat gaccgtctga tccacggcca
    89521 ggtcactacc gtctggtcga aagtggctaa tgcccagcac attattatct gcaatgacga
    89581 agtttataac gatgaagtgc gccggacatt attgcgtcag gctgcgccac cgggaatgaa
    89641 ggttaacgtg gtcaatattg aaaaagccgt cgcggtttat cacaacccgc aatatcagga
    89701 cgaaaccgtt ttttatcttt tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg
    89761 ggtaaaaata gacaccctga atattggcgg catggcctgg cggcccggta aaaaacagtt
    89821 aaccaaagcg gtttcattag atgatgatga tattaacgca tttcatgagt taaataatct
    89881 tggcgtcata ctggatttac gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa
    89941 aataaacgaa cagttaatcg caaattaaat aatagcgcct gtgtacatat gctttaacag
    90001 gcagggatga cgacacttaa aaggtgacaa atcatggaaa taagtaccct acaaatcatt
    90061 gctatatttc ttttttcctg tattgccgga atgggcagcg tgctggatga atttcagact
    90121 catcgtccgt taattgcctg tacggtgatt ggtttaattc tcggtgattt aaaaaccgga
    90181 attatgctcg gtggtacgct ggaattgata gctctcggct ggatgaacgt cggcgcggcg
    90241 caatctccgg attctgcact cgccagcata atctccgcca ttctggttat cgttggtcag
    90301 caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg
    90361 acggtgtttg cccgtaccat caccgtagtg ttccagcacg cggcggataa agcagcggaa
    90421 gaagcacggt ttcgcactct cgatattctg catgtctccg cgcttggcgt gcaggcactg
    90481 cgcgttgcta ttccggcact gattgtctcg ctgttcgtca gcgctgatat ggtgagcaat
    90541 atgctgagcg ccattcccga atttgtgacc cgtggactgc agattgctgg cggttttatc
    90601 gtggtggtcg gttacgccat ggtgcttcgc atgatgggcg tgaaatattt gatgcctttc
    90661 tttttcctcg gtttcctcgc aggtggctac ctcgatctca gtctgctggc gttcggtggc
    90721 gtcggcgtga tcatggccct gctctacatc cagctaaatc cacagtggcg taaagctgaa
    90781 cctcaactcc aggccaccgc ctccaccgcc cttgaccaac ttgacgatta acggagccat
    90841 catggaacag agaaaaatta cacgcagcga tctggtgagc atgtttctgc gctccaacct
    90901 gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggtttttgct acgacatgat
    90961 ccccgccatc aagcgacttt acccattaaa agaggatcag gttgcggcgc tcaggcgaca
    91021 cctggtgttc ttcaatacca cgccagccgt atgtggcccg gtcatcggcg tcactgccgc
    91081 catggaagag gcgcgggcca acggcgcgga aattgatgac ggtaccatta acggcatcaa
    91141 agtcggtctg atgggaccat tggcaggagt tggcgatcca ctggtctggg gaacgctgcg
    91201 cccgattacc gccgcgctcg gcgcatctct ggcactttcg ggcaacattc tcggcccgct
    91261 gctgtttttc tttattttca atgcggtgcg tctggcgatg aagtggtatg gcctacagct
    91321 cggctttcgc aaaggggtga atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact
    91381 caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc gtgctggtga ccaaatggac
    91441 gtcaatcaac gtaccgttgc tggtttcaca aacgcatgcc gccgatggct ccaccgtcac
    91501 catgaccgtg cagaacattc tcgaccaact ttgccctggt ttgctggcgc tcggtctgac
    91561 gctgctgatg gttcgtctgc tcaacaaaaa aattaacccg gtatggctga ttttcgccct
    91621 gtttggttta gggattatcg gtaatgcgct gggcttcctg tcctgattct tcgccccggc
    91681 acgactgccg gggtcatcgc tcaacatgag gtggtttatg aaaacaacag ctctgcgtct
    91741 ttatggcaaa cgtgacctgc gtctggaaac ctttgacctt cctgaaatgc aggaggatga
    91801 aatcctcgca acggtggtca ctgacagcct gtgcctctct tcctggaaag aggccaatct
    91861 gggtgaaaac cataaaaaag tacccgacga tgtggcgacc aaccccatca tcatcggcca
    91921 cgagttttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat tccagccggg
    91981 tcagcgttat gtgattcagg ccaacctgca actccccgac cgcccggatt gccccggcta
    92041 ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt attcccaacg aggtcatgga
    92101 acaagattgc ctgctggcat acgacggcga aacctatttt gaaggctcgc tggttgaacc
    92161 gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag gtagttataa
    92221 ccacacgatg gggattcgcc cgcaagggcg cacgctgatc ctcggcggca ccggcccgat
    92281 ggggctgctg gcgattgatt atgcgttgca tggaccggtt aacccgtcgc tgctcgtcat
    92341 taccgatacc gacaacgaca aattgagtta tgcacgcaag cactatccat cagaaccgca
    92401 aacactgatt cattatctca atgccgccga tgcagcattt gatacgttaa tggcactgag
    92461 tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac tggtgactct
    92521 cgcctcttcc ttgctggcga cagatggttg tctgaatttc ttcgccggac cgcaggataa
    92581 gcacttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc actacgtggg
    92641 cacgtcaggc ggcaataccg acgacatgcg cgcagcggtc aaattgattg aagagaaaaa
    92701 agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg gcgaaaccac
    92761 gcttgaattg cctgccatcg gcggcggcaa aaagctggtg tataccggga aatacctgcc
    92821 gcttatgtca ctcacgcaga ttcaggatca agcactggcg gcaattctgg cgcgtcatca
    92881 ggggatctgg tccggtgagg cggagcaata cctgctcgcc catgcagagg caatttccca
    92941 tgattaatcg cgctacttaa ctctgcatgt cgctggctgg tcgccgcggc aacttcgcga
    93001 cctgatgtgc ggctgattgc tgaagcggca caatgtgcac gagaaggata aaaaaacatc
    93061 agcgccttaa ggggcgctca aattggtggc aaagagcaca ttgtttatgc cggatgcgga
    93121 atgaagcctt attcggccta cataatcttg aaaattcaat atattgcagt tatctggtag
    93181 gccaggtaag cgtagcgcat caggcaatcc tgtgtttgtc atcagtctca gcgcctgtgc
    93241 aatatgtgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcattcctt
    93301 ccgtgttttc ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct
    93361 gccgggtaaa gcctggctgg aacacgcgct gccgctgatt gctgaacagt tgcagggtcg
    93421 ccgttctgcg gtatttattc ctttcgcggg cgtaacgcag acctgggatg attacacagc
    93481 gaaaacggct gcggttctcg ctccgctggg tgtttctgtc accggtattc atagcgttgt
    93541 cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt gtcggcggcg gtaatacttt
    93601 ccagttgctg aaacagtgcc gcgagcgcgg actgctggca ccaattactg acgtggttaa
    93661 acgtggcgcg ctgtatattg gctggagcgc tggcgctaac cttgcttgcc caaccattcg
    93721 taccaccaac gatatgccga ttgtcgatcc acaaggtttc gatgcgctga atctgttccc
    93781 gttgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg
    93841 tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa ctcacaatta ttggtctacc
    93901 ggaaggtaac tggatcacgg tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac
    93961 ttatgtgttt aaggctggtg aagaagcggt tccgctggaa gcgggtcacc gtttttaagt
    94021 gaattgccgg atgcatgatc cggcatcttg tacatagtgc ctgatgcgac gctttacacg
    94081 tcttatcagg ccttcagggt cacggtatta atactcgtcc cgtccttcgt cttcatccgg
    94141 ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcagacgct gcatatctcc
    94201 cagtaagcct aaatgtagcg aactggtttc aatactctgc acgttttgct gatgcaggcg
    94261 atcgacgtga gcatgggaat aacggcgatt aagaatgcga aagcgatgct tgctgcggcg
    94321 caaacgacgg gcgctggtaa catcgccaga gaagaaaacc gacattgcca gttttaaatt
    94381 actgagcaat tgctcataga gcgcatccag ttccttcaag ccatcaagcg aaaatgcccg
    94441 ccgtgctgcc agcgatttat cggcaatttc actccccatg cgctcgacga tatcggaggc
    94501 ctgttcaagg ttgagcgaca tttcgatgat ctccgcccag cggcgcgact cttcttctgc
    94561 cagttcctct tttggcatcc gcgccagata cagcttaatg gcggtataga gaacgttgat
    94621 gtcatcagcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca tcactttatt
    94681 caacccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc gggcagcgtt
    94741 cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg
    94801 ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac
    94861 aaatggcagc atgaccaggc agcgtacaag gttgtagaag acgtggaaat agatcaccaa
    94921 ttccgctttt ggcaatggca acttccccat tgtctctgcc agcaaatgaa caaacggcag
    94981 gataatcagg ctacccacca gcttaaacag caggctaccc agcgcgacac ggcgggctgc
    95041 ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt ttgcaccaat
    95101 caccagacaa agcgccactg ggaaagagat aatgcctgcg gcggtcagag tcgcggtcag
    95161 cagtaccgcg gcaaggctgg agtagctgat aatggcgaac atcgcaccaa tcagcgcatc
    95221 cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg cctgggtgat
    95281 cggcgttacg gcctgcacaa tcaactccag cgccagcaaa atcaacccaa gacaaatacc
    95341 gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat
    95401 aaaaataagt aatggtgaca gccaggataa gtcgaaggtg agaatacgcg ccattagcgc
    95461 cgtcccgaca tctgcaccca gcacaatcac cagagccggt gcgagggcta ccagatcctg
    95521 agcgacgaac gaggtcacca gcatagtggt ggcattactg ctctgcacca gtgcggtaac
    95581 gcctatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg
    95641 caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc cccagaccag
    95701 cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt ctccttatcg
    95761 ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga ctggcatctt
    95821 tactgtgcct tttgaccttc gtttttccgg ctaagattat tccagcctgt ttccagtata
    95881 agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg ttgtaaggaa
    95941 aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca gccagcgctc
    96001 aacatctgag acgctcatac ctttacggcg ggcatagtct tcaacctgat cgcgctggat
    96061 ttgcgccacc gcgtaatact tgctgtccgg gtggctgaag taccaacccg aaaccgatgc
    96121 accaggccac atggcgaaag attctgtgag tttcatgcca gtgtgttttt ccacttccag
    96181 cagctcccag atggtggctt tttccgtatg ttccgggcag gccggataac ccggtgccgg
    96241 acggatgccc tggtagtttt cgcggatcag ctcttcgttg ctgaggttct cgttcggcgc
    96301 atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg cttcggctaa
    96361 acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc
    96421 atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga tgtaatctgc
    96481 tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga agccggtttt
    96541 ttcggtctgt tgacgcagat ggtggctgac gttgatcaca tgggtacgcg tttcgtcacg
    96601 gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca cgccacgcgg
    96661 attcagcgtt ttttcggcgc ttaatttatc cagcatgtcg ttggcgtctt taaacagccg
    96721 ctgcgcctca acgcccacca cttcatcttc cagaatgcgc ggatacttcc cggacagcga
    96781 ccaggtcata aagaacggtg tccagtcgat gtaattacgc agcgtttcga tgctggcttc
    96841 gacttcctgc acaccgagac ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc
    96901 gaagtcgtta tcacgcgccg cttccagcgt gaccggtggt gtgcgtggtt tctttcgtcc
    96961 gtgctgaata cgcacggttt cgtactcctt gcgggtacga gcaacgaaat catcgcgctg
    97021 agtgtcggaa agcaacgccg ccaccacacc aacggtgcgt gaagcgttct gcacatacac
    97081 cgtcgggccg ctgtagttct gctcaatttt caccgccgtg tgcgcttttg aggtggtcgc
    97141 gccgccaatc aatagcggaa tggtgaagcc ctgacgctcc atctctttcg ccacgttaac
    97201 catctcgtcc agcgacggcg tgatcagccc cgaaaggcca atcaaatcag cattcacttc
    97261 tttagcggta cggagaattt tttccgcagg caccatcacg ccgagatcga caatttcgta
    97321 gttgttacat tgcagcacca cgccaacgat atttttaccg atgtcgtgga catcgccctt
    97381 cactgtggcg atcaccatct tgccgttggt tttaccctgc tctttgctgg cttcaatgaa
    97441 cggctcgaga tacgccaccg cctgtttcat gacgcgcgcc gatttcacca cctgcggcag
    97501 gaacattttg ccctcgccaa acaggtcgcc gaccacgttc atgccgtcca tcaacgggcc
    97561 ttcaatcact tcaatcggac gcgtagcctg ctggcgggct tcttcggtat cctgctcgat
    97621 aaactcggta atgcctttga ccagcgagta ttccagacgt ttattcactt cccacgagcg
    97681 ccactctgcc tgctgggtgt tagcggtgtc gtcggttttg ctgccgcgat atttctcggc
    97741 aagctcgagt aaacgctcgg tgccatcgtc gcgacggttg aggatcacat cctccaccgc
    97801 gtcgcgtagc tcggtaggca ggtcgtcgta aatcgccagt tgcccggcgt tgacgatccc
    97861 catatccatg ccattgcgaa tagcgtagta gaggaacacc gcgtgaatgg cttcgcgcac
    97921 cggatcgttg ccacggaatg agaaagaaac gttagaaacg ccgccggaaa tcagtgcgtg
    97981 cggcagttca cgtttgatgt cttcacacgc gccgataaag tcctgcgcgt aattgttgtg
    98041 ctcttcaatg ccagttgcga ccgcgaagat gttcgggtcg aagatgatat cttcagccgg
    98101 gaagccaacc tcttcggtga ggattttgta cgcccgacgg caaatctcga ttttccgtgc
    98161 gcgggtatcg gcctggccct gttcgtcaaa agccattacc acgactgccg caccgtagcg
    98221 acgcaacagt ttcgcgtgat ggataaaggc gtcgacgccc tctttcatcg agatagagtt
    98281 aacaatgcct ttgccctgga tacacttcag acctttttca ataacgtccc attttgagga
    98341 gtcgatcatt atcggcacgc gagcgatatc cggttcaccg gcaatcagat tgagaaaacg
    98401 caccatcgcc gcttcggcgt cgagcatccc ttcatccatg ttgatatcga taatctgcgc
    98461 accgttttcc acctgctgac gcgccacatc cagcgcctcg ctgtatttct cttctttgat
    98521 caggcgcttg aacttagcgg aaccggtgac gttggtgcgt tcacccacgt tcacaaacag
    98581 gctatcttcg ccaatgttca gcggctccag gccggacaaa cggcaggcta cggggatttc
    98641 cggcagtttg cgcggcgcta atccttctac tgcacgactc atcgcggcaa tatgttgtgg
    98701 cgtggtgcca cagcagccgc cgacgatatt gagaaagccc gcttgcgccc attcacgtat
    98761 ctgttttgcc atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg gtagcccggc
    98821 gttcgggtgc gctgtgacgt agcattccgc aatccgtgat aattcctgca cgtactggcg
    98881 cagttcgtct ggccccagcg cacagttaag gccgaaagtc agcgcttcgg cgtggcgcag
    98941 agagttataa aacgcttcgg tggtctgccc ggagagcgtg cgcccggagg cgtcggtgat
    99001 ggtgccggag atcataatcg gcagctcaac gcccagcgct tcaaactccg ttttcaccgc
    99061 aaagaccgcc gctttggcgt taagggtgtc gaaaacggtt tcaatcagaa tcaggtcggc
    99121 accaccttcc accagcgctt tggtggactc tcgataagcc gccaccagcc cgtcaaaagt
    99181 gatattacga aatgccggat cgttgacatc cggagaaata gacgccgtgc ggttggtcgg
    99241 gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg cgcgcggtcc actcatcagc
    99301 acaggcgcgc gccagtttcg ccgccgcaaa gttgatttcc gccgacagga attccatctg
    99361 gtaatccgcc atcgcaatgg ttgtggagtt gaaggtgttg gtttcgatga tatccgcgcc
    99421 cgcttcaaag taggcgttgt ggatagcggc gatcacttcc ggtttgctga gcaccagcag
    99481 gtcgttgttg cctttgagat cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc
    99541 ttcgttcagt cgatagctct ggatcatggt gcccataccg ccgtccagca ccagaatacg
    99601 ttcatttaac tgcgcacgca gttgttccac tttgctgctc acacttgctc ccgacacgct
    99661 caacccagat ttaataaaaa ttcaacaaac catactggca taaacgcatc tgtggtaaaa
    99721 gcgaccacca cgcaacatga gatttgttca acattaactc atcggatcag ttcagcaact
    99781 attgcattag ctaacaataa aaatgaaaat gatttccacg atacagaaaa aggagactgt
    99841 catggtcgca cccattcccg caaaacgcgg cagaaaaccc gccgttgcca ccgcaccagc
    99901 gactggacag gttcagtctt taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc
    99961 caatggcagt gtggcactca cggaactggc acagcaagcc gggttaccca attccacgac
   100021 ccaccgcctg ctaaccacga tgcagcaaca gggctttgtc cgtcaggtcg gcgaactggg
   100081 tcactgggca atcggcgcac acgcctttat ggtcggtagc agctttctcc agagccgtaa
   100141 tttgttagcg attgttcacc ctatcctgcg caatctaatg gaagagtctg gcgaaacggt
   100201 caatatggcg gtgcttgatc aaagcaatca cgaagcgatt attatcgacc aggtacagtg
   100261 tactcacctg atgcgaatgt ccgcgcctat cggcggtaaa ttgccgatgc acgcttccgg
   100321 tgcgggtaaa gcctttctgg cccaactgag cgaagaacag gtgacgaagc tgctgcaccg
   100381 caaagggtta catgcctata cccacgcaac gctggtgtct cctgtgcatt taaaagaaga
   100441 tctcgcccaa acgcgcaaac ggggttattc atttgacgat gaggaacatg cgctggggct
   100501 gcgttgcctt gcagcgtgta ttttcgatga gcaccgcgaa ccgtttgccg caatttcaat
   100561 ttccggaccg atttcacgta ttaccgatga ccgcgtgacc gagtttggcg cgatggtgat
   100621 taaagcggcg aaggaagtga cgctggcgta cggtggaata cgctgacttt ttcgcctctg
   100681 atttatacga atcgaacgct aaaccgctgc ttgcgccgat acgcataaac atcttccaca
   100741 tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caagtcggcg
   100801 tgcatctctt caaatagcgg accaatacgc gggtcggcac ataaccagtg acggaactct
   100861 tccgggaaaa catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcctcc
   100921 gggtagcgcg gcggcgggat gtcgcggaag ttcacttccg tcatgtagca aatttcgtcg
   100981 taatcataaa acaccacgcg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg
   101041 ccagggaaaa tattagcggc ggcaagctgg cgaatagcgt taccgtattc ttcaatggca
   101101 tcgcgcaact gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc
   101161 tcaatataaa gatggcgaat cacaatttgt tcgccgagat cggtgatttt ttccgctgct
   101221 tcctggagca gtaatgccat taatgccggg gaaatatgcc gcttctccag cacaaagttt
   101281 tcaaactcct gggtgtccgc cattcggccc acgcgatcgt gctctttcac cagttgatag
   101341 caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc tttgatgact
   101401 ttgaataccc gatcaaagcc cggcagcgta aacaccagca tcaccatgcc acgaataccc
   101461 ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc gcggtagctt
   101521 tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcggt ggttttacct
   101581 ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata aaccataaaa
   101641 taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tggtcgtcag gcaggtatca
   101701 ataaataact cgccgtcgtc cgtctggtgg atcggcagca aaaatggcaa tgtgccggaa
   101761 ggcgtgatca gattgcctac cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc
   101821 acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag atggcgaacg
   101881 atgtaatgaa tgtcacggct tttattctgc caacgcagac gcagcggcag atcgctgata
   101941 acgcgcatca gcagagattc ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg
   102001 ggaatggtac gaaaacggcg ctctggctgg gagctaaaga taaaaagccg ctcgggagta
   102061 agcgagcggt ggtcaaataa cctacagtac acggagttga aaaagctctc cgcaatctcg
   102121 aagcgcgggt aatccggcaa cagccgcgtg taatgctctt tgacgcgcag taaaaatgcc
   102181 gcgtccgtgc tttggccgtt ggtaatgcag cgcagttgct ccacgaccag acccacgtga
   102241 tgatcgtaaa gatgaatacg gtttttcatc gcctgctgga cagcatgcca gtcggcttgc
   102301 tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg agcgtcgaaa
   102361 ccttgcaaaa tggtttgtgc aatcagtaat tccaggccac gcggcatttg ttgccctcgt
   102421 cgggagcaga gaattgccgg ataagacgcg taagcgtcac atcaggcgct gattgccgga
   102481 tgcggcgtag acgccttatc cggcctacgt tcggcaacgg ctgtaggcat gataagacgc
   102541 gcaagcgtcg catcaggcgc tgattgccgg atgcggcgtg aacgccttat ccggcctaca
   102601 gtcagcaacg tttatcggtt gcttagaact gcgattcttc ggtggagcca gtcagtgcgg
   102661 taaccgacga cgtgccgccc tgaataatgg tcgtcacttt atcgaagtaa cctgtaccca
   102721 cttcctgttg gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct
   102781 gcactttctc aacatagtgc ttcataccct cgccctgggc ataggcgttt gccaggtcaa
   102841 acatgttgaa ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca
   102901 tatccgacag ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt
   102961 tgaacgacgg cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatggctt
   103021 gtgcaaagcg acgcgccagt tccaggtccg gcgtggaggt ttcacaccag accaggtcag
   103081 catatggcgc atacgccagg ccacggctga tcgcttgctc aatacccgca tgagtacgga
   103141 agaagccttc actggtacgc tcgccagtaa taaattcgct gtcatatggg tcgcaatcgg
   103201 aggtaatcag atccgccgca tcggcatcgg tacgagcaac cagcagggtt ggaacgcccg
   103261 tcacatcagc tgccagacgc gccgcgacca gtttctgaat agcttcctga gttggcacca
   103321 aaactttgcc gcccatgtga ccacatttct tcactgatgc cagctgatct tcgaagtgaa
   103381 ctgccgctgc accggcttca atcatcgctt tcatcagttc aaaggcattc agtacaccgc
   103441 caaaaccggc ctccgcatcg gcgacgatcg gcaggaagta atcgacatag cgcggatcgc
   103501 ccggctcaat gcccgcggac cattggatct gatccgcacg gcggaaagta ttgttgatcc
   103561 gctccaccac cgctggcacc gagtttgccg gatagagcga ctgatccgga tacatgctgg
   103621 ccgccaggtt agcgtccgcc gctacctgcc atcccgacag atagactgct tcaatacccg
   103681 ctttcgcctg ttgcagcgcc tgaccgccag tcagtgcacc gaggctgttg atgtagcctt
   103741 ttttcgactc gccgtgcagc agacgccaca ttttcgccgc gcccagttgc gccagggtgc
   103801 attccggatt gactgaaccg cgtaatttca ccacatcttc cgcgctgtac gggcgagtaa
   103861 tgccttccca acgcggttga gtccactctt tctgtaattc ttcaatttgt tgtgtacggg
   103921 ttttcatgtg cagatgctcc atagttatgt ggtggtttac gctaacaggc ggtagcctgg
   103981 cagggtcagg aaatcaatta actcatcgga agtggtgatc tgttccatca agcgtgcggc
   104041 atcgtcaaaa cgcccctggg agaaacgttc ttcgcccagt tcgctggcaa tgactttcat
   104101 ctcttcgccc agcatctggc ggaacaaggc tttagtcacc ggtttgccat tgctcaacgt
   104161 tttttgatga tggatccact gccagattga ggtacgggaa atttcagccg tcgccgcatc
   104221 ttccatcagg ccataaatcg gcacacagcc attgccagag atccacgctt cgatgtactg
   104281 cacagccacg cgaatgttgg cgcgcatacc ttcttcggtg cgttcaccat cacaaggtgc
   104341 cagcagctga tcggcagtaa tcggcgcgtc ttgttcgcgc atcacttcaa gctgattttt
   104401 acgggagccg agaatgtcgt tgaataccgc cattgccgta tccgcaaggc ctgggtgggc
   104461 aatccatgtg ccatcgtgac cgttattagc ttccagcgat ttatccgctt ttactttgtt
   104521 gagcacctgg ttattgcgct cttcatcttt gctcggaata aatgccgcca taccgcccat
   104581 cgcaaaagca ccgcgtttat ggcaggtttt aatcaacagg cgtgagtaag cattcaggaa
   104641 gggtttatcc atcgtcactg cctgtctatc tggcaggacg cgatcgggat agtttttcaa
   104701 tgttttgata taactgaaga tgtaatccca acgaccgcag ttcagaccaa caatatggtc
   104761 acgcagcgcg tgaaggattt catccatctg gaacacggcg ggcagcgttt caatcagcaa
   104821 cgtcgccttg atggtgccgc gcggcagatt aaagcgatct tctgcatagc tgaagacttc
   104881 gctccaccag gccgcttcct gccaggactg ggttttcggc agatagaaat agggaccgct
   104941 gccctttgcc aacagtgcct gatagttgtg gaagaaatag agcgcaaaat caaacaggct
   105001 accggggatt gcctcaccac gccaggtgac atgtttttcc ggcaagtgca gaccgcgtac
   105061 ccgacaaatc aaaaccgctg gattgggctt gagctgataa attttgcctg cttcattggt
   105121 atagctgatg gtgccgttaa ccgcatcgcg caggttaatt tgcccatcga tcactttgtt
   105181 ccagtctggt gccagtgaat cttcgaaatc ggccataaag actttcacat tggcgttgag
   105241 cgcgttgatc accatcttgc gctcaaccgg gccggtaatc tctacccgac gatcttctaa
   105301 gtccgcagga atcccgcgaa ttttccagtc agcatcacga atggaagctg tttccgaaat
   105361 aaaatcaggc aacgttccgt tatcgatatc ctgctgctgc tgaatgcgcg ctgccagaag
   105421 tttattgcgt tgtggcgtaa aatgcgtcac cagctcagtc agaaattcta ccgcttcggc
   105481 agtaagaata tgcttctcct gctcgccata cggccttgtg aaagccaatt catcggttgt
   105541 tgttgcctgt tcagtcatcg tgcagctcct cgtcatggat ccgaaaactt ccccactgtg
   105601 aacgaaggat cgttgtgcgc ttttcgttca gcacaactaa gactactcat ttaaaatgca
   105661 aaatcaaaaa caatttccat tttaattaaa aatacttgat aacttattga taacaatatg
   105721 aataaaattt attcacctac tgaggtgcgt ttcggaaata aaaaaggcac ccgaaggtgc
   105781 ctgaggtaag gtgctgaatc gcttaacgat cgactatcac agaagattaa tccagcgttg
   105841 ggttcatgtg ccgtagatcg tatggcgtga tctggtagac gtaatagttg agccagttgg
   105901 taaacagcaa attaccgtga ctacgccagc tcgctcgcgg tgtattttgc ggatcattat
   105961 gcgggaaata gttatacggt acatccgggt ctagtccggc ttccacatcg cggaaaaatt
   106021 cctgcgccag cgtttgcgca tcatattcgg gatggcccgt cacaaaggca attcgcttat
   106081 ctttgctggc aaacagatag gcatcccctt cttctgtctc cgccagaatt tccagatcgg
   106141 tataatcacg aatcaacgct gccggaaagt cagcatagcg cgaatgtggt gccaggaatg
   106201 aatcatcaaa gccacgcgtc agaagcgcat gaggatggag aatatgatgc tcgtaaacgc
   106261 cagagagttt gtcggtgcgg gtttgcttag gaatgccgta gaggatattg agcgcggcct
   106321 ggaccgccca acagacaaac agcgtcgagg tgacgtgatc tttcgaccac tccagcacct
   106381 gtttgatctg cggccagtaa gcaacatcat taaactccac caggcccagc ggcgcgccgg
   106441 taactatcag accatcaaag ttttgttcct gaatatcttc aaagttacag tagaagttat
   106501 tcagatgctc tgcgggcgtg ttgcgcgatt cacgggaatc gatgcgcaac agctgaatat
   106561 cgacctgcaa aggtgagttt gaaagcaggc gcagaaactg attttcagtt tcgatcttct
   106621 tcggcatcag gttaaggata agcaccttta gcggacgaat ttcctgacca gacgcacgag
   106681 aagttgtcat cacaaagacg ttttcttcac gcaagaaatt gacggcgggt agctcgtccg
   106741 gcacacgaat cggcataacc tgattacctc actacatacg cttatacgtt tagacatcca
   106801 gatagctgaa gataaccaga aaatttgcca atgtcgagcc tgcatattga aggtgagaaa
   106861 gtttcaagga aaaggctacg ttagaatata agaatgacga aaaggagaga aaatggttat
   106921 tagtattcgc cgctcacggc atgaggaagg ggaggagctc gttgcgattt ggtgtcgttc
   106981 tgtcgatgcc actcacgatt ttttatcagc agcgtatcgg gccgagctgg aggagctagt
   107041 tcgttccttc ctgccggaag cgccgttgtg ggtcgcggtt aatgagcggg atcggccggt
   107101 tgggtttatg ttgttaagtg ggcagcatat ggatgcgctg tttatcgatc ctgatgtgcg
   107161 cggctgcggc gtgggtcgga tgctggtgga acatgcgctc tcgatggcac cggaactgac
   107221 aaccaatgtt aatgagcaaa ataagcaggc tgttgggttc tataagaaga tgggttttaa
   107281 ggttacggga cgctctgagg tggacgattt ggggaaaccg tatccattgc tgaatctggc
   107341 atatgtaggg gaataagctg ccccctatcg tgctatcgtg cttgcgtaag ctggagcaga
   107401 cgcgccagcg tcgcatccgg cattttttct gcgccaatgc aaaaaggcca tcctttcgga
   107461 tggccttt
//
LOCUS       DAZGTJ010000005       102945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000005 DAZGTJ010000000
VERSION     DAZGTJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.005"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(150..722)
                     /gene="gmhB"
                     /locus_tag="ACHK8E_000467"
     CDS             complement(150..722)
                     /gene="gmhB"
                     /locus_tag="ACHK8E_000467"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP0342785.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            910..1941
                     /gene="metN"
                     /locus_tag="ACHK8E_000468"
     CDS             910..1941
                     /gene="metN"
                     /locus_tag="ACHK8E_000468"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP0342786.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            1934..2587
                     /gene="metI"
                     /locus_tag="ACHK8E_000469"
     CDS             1934..2587
                     /gene="metI"
                     /locus_tag="ACHK8E_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP0342787.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2627..3442
                     /gene="metQ"
                     /locus_tag="ACHK8E_000470"
     CDS             2627..3442
                     /gene="metQ"
                     /locus_tag="ACHK8E_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP0342788.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3560..3964
                     /gene="rcsF"
                     /locus_tag="ACHK8E_000471"
     CDS             3560..3964
                     /gene="rcsF"
                     /locus_tag="ACHK8E_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP0342789.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            3961..4668
                     /gene="tsaA"
                     /locus_tag="ACHK8E_000472"
     CDS             3961..4668
                     /gene="tsaA"
                     /locus_tag="ACHK8E_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP0342790.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4779..6497
                     /gene="proS"
                     /locus_tag="ACHK8E_000473"
     CDS             4779..6497
                     /gene="proS"
                     /locus_tag="ACHK8E_000473"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP0342791.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6550..7374
                     /gene="yaeF"
                     /locus_tag="ACHK8E_000474"
     CDS             6550..7374
                     /gene="yaeF"
                     /locus_tag="ACHK8E_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP0342792.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(7404..8114)
                     /gene="nlpE"
                     /locus_tag="ACHK8E_000475"
                     /gene_synonym="cutF"
     CDS             complement(7404..8114)
                     /gene="nlpE"
                     /locus_tag="ACHK8E_000475"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP0342793.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8128..8550)
                     /gene="arfB"
                     /locus_tag="ACHK8E_000476"
     CDS             complement(8128..8550)
                     /gene="arfB"
                     /locus_tag="ACHK8E_000476"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP0342794.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(8547..9092)
                     /gene="yaeQ"
                     /locus_tag="ACHK8E_000477"
     CDS             complement(8547..9092)
                     /gene="yaeQ"
                     /locus_tag="ACHK8E_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP0342795.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9258..9458
                     /gene="yaeP"
                     /locus_tag="ACHK8E_000478"
     CDS             9258..9458
                     /gene="yaeP"
                     /locus_tag="ACHK8E_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP0342796.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9445..9705
                     /gene="rof"
                     /locus_tag="ACHK8E_000479"
     CDS             9445..9705
                     /gene="rof"
                     /locus_tag="ACHK8E_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP0342797.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9754..11052)
                     /gene="tilS"
                     /locus_tag="ACHK8E_000480"
     CDS             complement(9754..11052)
                     /gene="tilS"
                     /locus_tag="ACHK8E_000480"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP0342798.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(11117..11506)
                     /gene="yaeR"
                     /locus_tag="ACHK8E_000481"
     CDS             complement(11117..11506)
                     /gene="yaeR"
                     /locus_tag="ACHK8E_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0342799.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11563..13704)
                     /gene="ldcC"
                     /locus_tag="ACHK8E_000482"
     CDS             complement(11563..13704)
                     /gene="ldcC"
                     /locus_tag="ACHK8E_000482"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP0342800.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13803..14762)
                     /gene="accA"
                     /locus_tag="ACHK8E_000483"
     CDS             complement(13803..14762)
                     /gene="accA"
                     /locus_tag="ACHK8E_000483"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP0342801.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14775..18257)
                     /gene="dnaE"
                     /locus_tag="ACHK8E_000484"
     CDS             complement(14775..18257)
                     /gene="dnaE"
                     /locus_tag="ACHK8E_000484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0342802.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18294..18890)
                     /gene="rnhB"
                     /locus_tag="ACHK8E_000485"
     CDS             complement(18294..18890)
                     /gene="rnhB"
                     /locus_tag="ACHK8E_000485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP0342803.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            complement(18887..20035)
                     /gene="lpxB"
                     /locus_tag="ACHK8E_000486"
     CDS             complement(18887..20035)
                     /gene="lpxB"
                     /locus_tag="ACHK8E_000486"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP0342804.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20035..20823)
                     /gene="lpxA"
                     /locus_tag="ACHK8E_000487"
     CDS             complement(20035..20823)
                     /gene="lpxA"
                     /locus_tag="ACHK8E_000487"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP0342805.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20827..21282)
                     /gene="fabZ"
                     /locus_tag="ACHK8E_000488"
     CDS             complement(20827..21282)
                     /gene="fabZ"
                     /locus_tag="ACHK8E_000488"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP0342806.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21387..22412)
                     /gene="lpxD"
                     /locus_tag="ACHK8E_000489"
     CDS             complement(21387..22412)
                     /gene="lpxD"
                     /locus_tag="ACHK8E_000489"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0342807.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22416..22901)
                     /gene="skp"
                     /locus_tag="ACHK8E_000490"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22416..22901)
                     /gene="skp"
                     /locus_tag="ACHK8E_000490"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP0342808.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23023..25455)
                     /gene="bamA"
                     /locus_tag="ACHK8E_000491"
     CDS             complement(23023..25455)
                     /gene="bamA"
                     /locus_tag="ACHK8E_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP0342809.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25485..26837)
                     /gene="rseP"
                     /locus_tag="ACHK8E_000492"
     CDS             complement(25485..26837)
                     /gene="rseP"
                     /locus_tag="ACHK8E_000492"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP0342810.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26849..27706)
                     /gene="cdsA"
                     /locus_tag="ACHK8E_000493"
     CDS             complement(26849..27706)
                     /gene="cdsA"
                     /locus_tag="ACHK8E_000493"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0342811.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27719..28477)
                     /gene="ispU"
                     /locus_tag="ACHK8E_000494"
                     /gene_synonym="uppS"
     CDS             complement(27719..28477)
                     /gene="ispU"
                     /locus_tag="ACHK8E_000494"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP0342812.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28666..29862)
                     /gene="ispC"
                     /locus_tag="ACHK8E_000495"
     CDS             complement(28666..29862)
                     /gene="ispC"
                     /locus_tag="ACHK8E_000495"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP0342813.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(29954..30511)
                     /gene="frr"
                     /locus_tag="ACHK8E_000496"
     CDS             complement(29954..30511)
                     /gene="frr"
                     /locus_tag="ACHK8E_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP0342814.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30803..31528)
                     /gene="pyrH"
                     /locus_tag="ACHK8E_000497"
     CDS             complement(30803..31528)
                     /gene="pyrH"
                     /locus_tag="ACHK8E_000497"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP0342815.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31675..32526)
                     /gene="tsf"
                     /locus_tag="ACHK8E_000498"
     CDS             complement(31675..32526)
                     /gene="tsf"
                     /locus_tag="ACHK8E_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP0342816.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32784..33509)
                     /gene="rpsB"
                     /locus_tag="ACHK8E_000499"
     CDS             complement(32784..33509)
                     /gene="rpsB"
                     /locus_tag="ACHK8E_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP0342817.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33877..34671
                     /gene="map"
                     /locus_tag="ACHK8E_000500"
     CDS             33877..34671
                     /gene="map"
                     /locus_tag="ACHK8E_000500"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP0342818.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34733..37405
                     /gene="glnD"
                     /locus_tag="ACHK8E_000501"
     CDS             34733..37405
                     /gene="glnD"
                     /locus_tag="ACHK8E_000501"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP0342819.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37436..38260
                     /gene="dapD"
                     /locus_tag="ACHK8E_000502"
     CDS             37436..38260
                     /gene="dapD"
                     /locus_tag="ACHK8E_000502"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP0342820.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38313..39125
                     /gene="yaeI"
                     /locus_tag="ACHK8E_000503"
     CDS             38313..39125
                     /gene="yaeI"
                     /locus_tag="ACHK8E_000503"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFP0342821.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            39283..39669
                     /gene="yaeH"
                     /locus_tag="ACHK8E_000504"
     CDS             39283..39669
                     /gene="yaeH"
                     /locus_tag="ACHK8E_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP0342822.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39784..40941)
                     /gene="cdaR"
                     /locus_tag="ACHK8E_000505"
     CDS             complement(39784..40941)
                     /gene="cdaR"
                     /locus_tag="ACHK8E_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP0342823.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(41096..42520)
                     /gene="degP"
                     /locus_tag="ACHK8E_000506"
     CDS             complement(41096..42520)
                     /gene="degP"
                     /locus_tag="ACHK8E_000506"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP0342824.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(42650..44167)
                     /gene="dgt"
                     /locus_tag="ACHK8E_000507"
     CDS             complement(42650..44167)
                     /gene="dgt"
                     /locus_tag="ACHK8E_000507"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP0342825.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            44251..44949
                     /gene="mtnN"
                     /locus_tag="ACHK8E_000508"
     CDS             44251..44949
                     /gene="mtnN"
                     /locus_tag="ACHK8E_000508"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP0342826.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44942..45742
                     /gene="btuF"
                     /locus_tag="ACHK8E_000509"
     CDS             44942..45742
                     /gene="btuF"
                     /locus_tag="ACHK8E_000509"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP0342827.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45780..46403
                     /gene="yadS"
                     /locus_tag="ACHK8E_000510"
     CDS             45780..46403
                     /gene="yadS"
                     /locus_tag="ACHK8E_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP0342828.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(46450..46794)
                     /gene="erpA"
                     /locus_tag="ACHK8E_000511"
     CDS             complement(46450..46794)
                     /gene="erpA"
                     /locus_tag="ACHK8E_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP0342829.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46787..46852)
                     /gene="yadW"
                     /locus_tag="ACHK8E_000512"
     CDS             complement(46787..46852)
                     /gene="yadW"
                     /locus_tag="ACHK8E_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP0342830.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46876..48297)
                     /gene="clcA"
                     /locus_tag="ACHK8E_000513"
     CDS             complement(46876..48297)
                     /gene="clcA"
                     /locus_tag="ACHK8E_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP0342831.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(48309..48356)
                     /gene="yadX"
                     /locus_tag="ACHK8E_000514"
     CDS             complement(48309..48356)
                     /gene="yadX"
                     /locus_tag="ACHK8E_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP0342832.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            48522..49802
                     /gene="hemL"
                     /locus_tag="ACHK8E_000515"
     CDS             48522..49802
                     /gene="hemL"
                     /locus_tag="ACHK8E_000515"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP0342833.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49837..51819)
                     /gene="fhuB"
                     /locus_tag="ACHK8E_000516"
     CDS             complement(49837..51819)
                     /gene="fhuB"
                     /locus_tag="ACHK8E_000516"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP0342834.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51816..52706)
                     /gene="fhuD"
                     /locus_tag="ACHK8E_000517"
     CDS             complement(51816..52706)
                     /gene="fhuD"
                     /locus_tag="ACHK8E_000517"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP0342835.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            complement(52706..53503)
                     /gene="fhuC"
                     /locus_tag="ACHK8E_000518"
     CDS             complement(52706..53503)
                     /gene="fhuC"
                     /locus_tag="ACHK8E_000518"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP0342836.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(53554..55743)
                     /gene="fhuA"
                     /locus_tag="ACHK8E_000519"
     CDS             complement(53554..55743)
                     /gene="fhuA"
                     /locus_tag="ACHK8E_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP0342837.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(55963..58500)
                     /gene="mrcB"
                     /locus_tag="ACHK8E_000520"
     CDS             complement(55963..58500)
                     /gene="mrcB"
                     /locus_tag="ACHK8E_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP0342838.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQ
                     LPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPF
                     DFPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVP
                     RSGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKN
                     LFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLY
                     YFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELY
                     DMLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDA
                     AEKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRS
                     IGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVML
                     VDALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQ
                     AFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRG
                     TGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGA
                     MSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQS
                     EMQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58594..61023)
                     /gene="hrpB"
                     /locus_tag="ACHK8E_000521"
     CDS             complement(58594..61023)
                     /gene="hrpB"
                     /locus_tag="ACHK8E_000521"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP0342839.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            61097..61627
                     /gene="thpR"
                     /locus_tag="ACHK8E_000522"
     CDS             61097..61627
                     /gene="thpR"
                     /locus_tag="ACHK8E_000522"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP0342840.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            61642..62346
                     /gene="sfsA"
                     /locus_tag="ACHK8E_000523"
     CDS             61642..62346
                     /gene="sfsA"
                     /locus_tag="ACHK8E_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP0342841.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            62524..62979
                     /gene="dksA"
                     /locus_tag="ACHK8E_000524"
     CDS             62524..62979
                     /gene="dksA"
                     /locus_tag="ACHK8E_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0342842.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            63046..63942
                     /gene="gluQRS"
                     /locus_tag="ACHK8E_000525"
     CDS             63046..63942
                     /gene="gluQRS"
                     /locus_tag="ACHK8E_000525"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP0342843.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            64002..65399
                     /gene="pcnB"
                     /locus_tag="ACHK8E_000526"
     CDS             64002..65399
                     /gene="pcnB"
                     /locus_tag="ACHK8E_000526"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP0342844.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            65396..65875
                     /gene="folK"
                     /locus_tag="ACHK8E_000527"
     CDS             65396..65875
                     /gene="folK"
                     /locus_tag="ACHK8E_000527"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP0342845.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGFNELDKW"
     gene            66237..66827
                     /locus_tag="ACHK8E_000528"
     CDS             66237..66827
                     /locus_tag="ACHK8E_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0342846.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            66938..67678
                     /gene="yadV"
                     /locus_tag="ACHK8E_000529"
     CDS             66938..67678
                     /gene="yadV"
                     /locus_tag="ACHK8E_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0342847.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            67713..70313
                     /gene="htrE"
                     /locus_tag="ACHK8E_000530"
     CDS             67713..70313
                     /gene="htrE"
                     /locus_tag="ACHK8E_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP0342848.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            70330..70902
                     /locus_tag="ACHK8E_000531"
     CDS             70330..70902
                     /locus_tag="ACHK8E_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0342849.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            70914..71522
                     /gene="yadL"
                     /locus_tag="ACHK8E_000532"
     CDS             70914..71522
                     /gene="yadL"
                     /locus_tag="ACHK8E_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP0342850.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            71549..72145
                     /locus_tag="ACHK8E_000533"
     CDS             71549..72145
                     /locus_tag="ACHK8E_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0342851.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            72171..73460
                     /locus_tag="ACHK8E_000534"
     CDS             72171..73460
                     /locus_tag="ACHK8E_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP0342852.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            73572..74366
                     /gene="panB"
                     /locus_tag="ACHK8E_000535"
     CDS             73572..74366
                     /gene="panB"
                     /locus_tag="ACHK8E_000535"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP0342853.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            74378..75229
                     /gene="panC"
                     /locus_tag="ACHK8E_000536"
     CDS             74378..75229
                     /gene="panC"
                     /locus_tag="ACHK8E_000536"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP0342854.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(75311..75526)
                     /locus_tag="ACHK8E_000537"
     CDS             complement(75311..75526)
                     /locus_tag="ACHK8E_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0342855.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(75595..76551)
                     /locus_tag="ACHK8E_000538"
     CDS             complement(75595..76551)
                     /locus_tag="ACHK8E_000538"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0342856.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     IQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            76825..77205
                     /gene="panD"
                     /locus_tag="ACHK8E_000539"
     CDS             76825..77205
                     /gene="panD"
                     /locus_tag="ACHK8E_000539"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP0342857.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(77209..78438)
                     /gene="yadE"
                     /locus_tag="ACHK8E_000540"
     CDS             complement(77209..78438)
                     /gene="yadE"
                     /locus_tag="ACHK8E_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0342858.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(78502..78942)
                     /gene="yadI"
                     /locus_tag="ACHK8E_000541"
     CDS             complement(78502..78942)
                     /gene="yadI"
                     /locus_tag="ACHK8E_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0342859.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(79047..79817)
                     /gene="yadH"
                     /locus_tag="ACHK8E_000542"
     CDS             complement(79047..79817)
                     /gene="yadH"
                     /locus_tag="ACHK8E_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0342860.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(79814..80740)
                     /gene="yadG"
                     /locus_tag="ACHK8E_000543"
     CDS             complement(79814..80740)
                     /gene="yadG"
                     /locus_tag="ACHK8E_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0342861.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKGAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            80849..81511
                     /gene="can"
                     /locus_tag="ACHK8E_000544"
     CDS             80849..81511
                     /gene="can"
                     /locus_tag="ACHK8E_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0342862.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(81552..82088)
                     /gene="hpt"
                     /locus_tag="ACHK8E_000545"
     CDS             complement(81552..82088)
                     /gene="hpt"
                     /locus_tag="ACHK8E_000545"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP0342863.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            82294..84684
                     /gene="gcd"
                     /locus_tag="ACHK8E_000546"
     CDS             82294..84684
                     /gene="gcd"
                     /locus_tag="ACHK8E_000546"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP0342864.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(84880..86430)
                     /gene="cueO"
                     /locus_tag="ACHK8E_000547"
     CDS             complement(84880..86430)
                     /gene="cueO"
                     /locus_tag="ACHK8E_000547"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP0342865.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            86596..86943
                     /gene="yacC"
                     /locus_tag="ACHK8E_000548"
     CDS             86596..86943
                     /gene="yacC"
                     /locus_tag="ACHK8E_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP0342866.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            87049..87915
                     /gene="speE"
                     /locus_tag="ACHK8E_000549"
     CDS             87049..87915
                     /gene="speE"
                     /locus_tag="ACHK8E_000549"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP0342867.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            87931..88725
                     /gene="speD"
                     /locus_tag="ACHK8E_000550"
     CDS             87931..88725
                     /gene="speD"
                     /locus_tag="ACHK8E_000550"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP0342868.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(88763..89125)
                     /gene="yacL"
                     /locus_tag="ACHK8E_000551"
     CDS             complement(88763..89125)
                     /gene="yacL"
                     /locus_tag="ACHK8E_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP0342869.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(89300..91897)
                     /gene="acnB"
                     /locus_tag="ACHK8E_000552"
     CDS             complement(89300..91897)
                     /gene="acnB"
                     /locus_tag="ACHK8E_000552"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP0342870.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            92252..94009
                     /gene="yacH"
                     /locus_tag="ACHK8E_000553"
     CDS             92252..94009
                     /gene="yacH"
                     /locus_tag="ACHK8E_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP0342871.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(94080..95504)
                     /gene="lpdA"
                     /locus_tag="ACHK8E_000554"
     CDS             complement(94080..95504)
                     /gene="lpdA"
                     /locus_tag="ACHK8E_000554"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP0342872.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(95829..97721)
                     /gene="aceF"
                     /locus_tag="ACHK8E_000555"
     CDS             complement(95829..97721)
                     /gene="aceF"
                     /locus_tag="ACHK8E_000555"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP0342873.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(97736..100399)
                     /gene="aceE"
                     /locus_tag="ACHK8E_000556"
     CDS             complement(97736..100399)
                     /gene="aceE"
                     /locus_tag="ACHK8E_000556"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP0342874.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(100560..101324)
                     /gene="pdhR"
                     /locus_tag="ACHK8E_000557"
     CDS             complement(100560..101324)
                     /gene="pdhR"
                     /locus_tag="ACHK8E_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP0342875.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(101780..102070)
                     /locus_tag="ACHK8E_000558"
     CDS             complement(101780..102070)
                     /locus_tag="ACHK8E_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP0342876.1"
                     /translation="MYNFKDKVEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
     gene            complement(102071..102310)
                     /locus_tag="ACHK8E_000559"
     CDS             complement(102071..102310)
                     /locus_tag="ACHK8E_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342877.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGPKEIVKKFH
                     MYHCVAIEYSGEIYDIDNLRRAHLICMMKFITGDS"
     gene            complement(102468..102761)
                     /locus_tag="ACHK8E_000560"
     CDS             complement(102468..102761)
                     /locus_tag="ACHK8E_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP0342878.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSTMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSKQSR"
     gene            complement(102764..>102945)
                     /locus_tag="ACHK8E_000561"
     CDS             complement(102764..>102945)
                     /locus_tag="ACHK8E_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0342879.1"
                     /translation="KQGLTAKAPLEGWRYGPKEIVKKFQIHHRVAIEYGGSVYDIDNL
                     RIVTPRLHDEIHYRR"
BASE COUNT        25253 a        27671 c        25039 g        24982 t
ORIGIN      
        1 gaatttcagg atttttctct ttaaccgaac cggctgtttg tgtgaagtga ttcacatccg
       61 ccgtgtcgat ggaggcgcat tatagggagt cggctcagga agacaagcgg aaaaatgcat
      121 ttttatttca accgctcatc ttttaatcat tacgccggtt tttgctgctt ttttatcgct
      181 tgcggcaggt ctgccaggct atttaacacc caatccgccg cgttttctgc ttcaggcgta
      241 acaggtttac ccgtacgcac cagcactttt gttcccacgt tcgccgcagc tgctgcctgc
      301 atatcttcta atttatcgcc caccatataa gaagcggcca tatcaatatg caaataatcg
      361 cgtgctgaca aaagcatccc cggatgtggt ttgcggcagt cgcagacctg gcgaaactct
      421 tcaacactac cctgcggatg atgcgggcaa taatagatac catccagatc gacatcgcgg
      481 tccgccagcg accagtccat ccactcggtc agcgtttcaa actgtgcttc agtaaattta
      541 ccgcgagcaa tgccagactg gttggttact accaccagcg caaagcccat tttttttagc
      601 tcgcgcatgg cgtcaataac accgtcgata aattcaaagt tgtcgatctc atggacatag
      661 ccgtgatcga cattaatggt gccatcacgg tcaagaaaaa ttgcgggtac gctcttcgcc
      721 accttttata gctccttaat aaggcatgtg acgctagtat cgcatgtttc gaccagcaag
      781 aaagtgctct tcgcataaac ctgattgatt tagacgtctg gatgccttaa catccatttc
      841 attgaaggcg ttgcccgttc caggcattcg aaatgccacg actaacttaa tgacgataat
      901 aaataatcaa tgataaaact ttcgaatatc accaaagtgt tccaccaggg cacccgcacc
      961 atccaggcgt tgaacaacgt cagcctgcat gtgccagctg gacaaattta tggcgttatc
     1021 ggtgcctctg gcgcgggtaa gagtacgctt atacgttgtg taaacctgct ggagcgccca
     1081 accgagggta gcgtgctggt cgatggccag gaactgacca cgctgtcaga atccgagttg
     1141 accaaagctc gccgccagat tggtatgatt ttccagcatt ttaacctgct ctcttcgcgt
     1201 actgtttttg gcaacgtggc tctgccgctg gagctggaca acacaccgaa agatgagatc
     1261 aaacgtcgcg tgacggaatt gctgtcattg gttggtcttg gcgataagca tgatagctac
     1321 ccgtcgaatc tttccggtgg gcagaaacag cgtgtggcga ttgcccgtgc gttagccagc
     1381 aatcccaaag tattgctgtg tgatgaggcc accagcgcgc tggatccggc aacgacacgt
     1441 tctattctcg aactgctgaa agacatcaac cgccgtctgg gtttgacgat tctgttgatc
     1501 acccacgaaa tggacgttgt gaagcgtatt tgtgattgcg tggcggtcat cagcaatggc
     1561 gaactgatcg agcaggacac ggtaagtgaa gtgttctcgc atccgaaaac gccgctggcg
     1621 cagaagttta ttcagtcgac cctgcatctg gatatcccgg aagattacca ggaacgtctg
     1681 caagcggagc cattcactga ctgcgtgccg atgttgcgtc tggagtttac cggtcaatcg
     1741 gtcgatgccc cactgctttc tgaaaccgcg cgtcgtttca acgtcaacaa caacattatt
     1801 agcgcgcaga tggattacgc cggtggcgtt aagttcggca tcatgctgac tgaaatgcac
     1861 ggcacacaac aagatacgca agccgccatt acctggctgc aggaacacca tgtaaaagta
     1921 gaggtactgg gttatgtctg agccgatgat gtggctgctg gttcgtggcg tatgggaaac
     1981 gctggcaatg accttcgtat ccggtttttt tggatttgtg attggtctgc cggttggcgt
     2041 cctgctttat gtcacgcgtc cggggcaaat tattgctaac gcgaagctgt atcgtaccgt
     2101 ttctgcgatt gtgaacattt tccgttccat cccgttcatt atcttgctgg tatggatgat
     2161 tccgtttacc cgcgttattg ttggtacatc gattggtttg caggcagcga ttgttccgtt
     2221 aaccgttggt gcagcaccgt ttattgcccg tatggtcgag aacgctctgc tggagatccc
     2281 aaccgggtta attgaagctt cccgcgcaat gggtgccacg ccaatgcaga tcgtccgtaa
     2341 agtgctgtta ccggaagcgt tgccgggtct ggtaaatgcg gcaactatca ccctgattac
     2401 cctggtcggt tattccgcga tgggtggtgc agtcggtgcc ggtggtttag gtcagattgg
     2461 ctatcagtat ggctacatcg gctacaacgc gacggtgatg aatacggtac tggtattgct
     2521 ggtcattctg gtttatttaa ttcagttcgc aggcgaccgc atcgtccggg ctgtcactcg
     2581 caagtaacgt tcaacacaac ataaataatt gaagaaggaa taaggtatgg cgttcaaatt
     2641 caaaaccttt gcggcagtgg gagccctgat tggatcactg gcactggtag gctgcggtca
     2701 ggatgaaaaa gatccaaacc acattaaagt cggcgtgatt gttggtgccg aacagcaggt
     2761 tgcagaagtc gcgcagaaag ttgcgaaaga caaatatggc ctggacgttg agctggtaac
     2821 attcaacgac tatgttctgc caaacgaagc attgagcaaa ggcgatatcg acgccaacgc
     2881 cttccagcat aaaccgtacc ttgatcagca actgaaagat cgtggctaca aactggtcgc
     2941 agtaggcaac acttttgttt atccgattgc aggttactcc aagaaaatca aatcactgga
     3001 tgaactgcag gatggttcgc aggttgccgt gccaaacgac ccaactaacc ttggtcgttc
     3061 actgctgctg ctgcaaaaag tgggcttgat caaactgaaa gatggcgttg gcctgctgcc
     3121 gaccgttctt gatgttgttg agaaccccaa aaatctgaaa attgttgagc tggaagcacc
     3181 gcagctgccg cgttctctgg acgacgcgca aatcgctctg gcagttatca ataccaccta
     3241 tgccagccag attggcctga ctccggcgaa agacggtatc tttgtcgaag ataaagagtc
     3301 cccgtacgta aacctgatcg taacgcgtga agataacaaa gacgccgaga acgtgaagaa
     3361 attcgtccag gcttatcagt ctgacgaagt ttacgaagca gcaaacaaag tgtttaacgg
     3421 cggcgctgtt aaaggctggt aatttttagg ctgtttccac aatttgtaat atcattcagg
     3481 acgggcgctt gcccgtcttg tcatttttac aagctcctga ttcaatattg acgttttgat
     3541 catacattga ggaaatacta tgcgtgcttt accgatctgt ttagtagcac tcatgctaag
     3601 cggctgttcc atgttaagca gatcccctgt cgaacccgtt caaagcactg caccccagcc
     3661 gaaagcggag tctgcaaaac cgaaagtgcc gcgcgccacg ccggtccgaa tttataccaa
     3721 tgcagaagaa ttagtcggca aaccgttccg cgatctcggt gaagtcagtg gcgactcttg
     3781 ccaagcctct aatcaggact ctccgccgag cattccaacc gcgcgtaagc ggatgcaaat
     3841 caacgcctcg aaaatgaaag ccaatgcggt attactgcat agctgcgaag tcaccagcgg
     3901 tacgccaggc tgctatcgtc aggctgtatg tatcggttct gcgcttaaca ttacggcgaa
     3961 atgagcagtt tccagttcga gcaaataggc gttattcgct cgccctataa agaaaaattc
     4021 gccgttccgc gccaaccagg tctggtaaaa agcgccaacg gcgaactgca tctcattgct
     4081 ccctacaacc aagccgacgc cgttcgcggc ctggaagcgt tcagccattt atggatactt
     4141 ttcgtctttc atcaaacgat ggaaggcggc tggcgtccga ctgtgcgtcc gcctcgtctt
     4201 ggcggtaatg ccagaatggg ggttttcgca acacgctcca cttttcgccc taacccaatt
     4261 ggtatgtcgc tggtagagct gaaagaggtt gtttgccata aagacagcgt gattctgaag
     4321 ctcggcagtc tggatctggt cgatggtacg ccggtagtgg atatcaaacc gtatctcccc
     4381 tttgccgaat cgcttccaaa tgccagtgcc agctatgcgc aaagtgcgcc agctgcagag
     4441 atggcggtga gttttaccgc agaggtcgaa aagcagcttt tgacgctgga gaagcgttat
     4501 ccgcagttaa cgctgtttat ccgcgaagta ctagcgcagg acccgcgccc ggcctatcgt
     4561 aaaggtgagg aaacgggcaa aacctatgcc gtctggctgc atgattttaa cgttcgctgg
     4621 cgcgtcaccg acgcaggttt tgaagtcttt gcgctggaac cgcgttaaat tcacgccctt
     4681 ctcttttgac atttcttttg cactggtaaa ctaaatcact ttttttgtcc caggctcacc
     4741 ttgagcctgt tctaccttcc aactggaacc gtaacaacat gcgtactagc caatacctgc
     4801 tctccactct caaggagaca cctgccgacg ccgaggtgat cagccatcag ctgatgctgc
     4861 gcgccgggat gatccgcaag ctggcctccg ggttatacac ctggctgccg accggcgtgc
     4921 gcgttctgaa aaaagtcgaa aacatcgtgc gtgaagagat gaacaacgcc ggtgcgattg
     4981 aggtgttaat gccggtggtt cagccatctg aactgtggca agagagtggt cgttgggaac
     5041 agtatggccc ggaattgctg cgtattgctg accgtggtga ccgtccgttc gtacttggcc
     5101 caactcatga agaagtgatt accgacctga ttcgtaacga gctgagctct tacaaacagc
     5161 tgccgctgaa cttctatcag atccagacta agttccgtga tgaagtgcgc ccgcgtttcg
     5221 gcgtcatgcg ttcccgcgaa ttcctgatga aagatgctta ctctttccat acctctcagg
     5281 aatccctgca ggaaacctac gatgcaatgt atgcggccta cagcaaaatc ttcagccgca
     5341 tggggctgga tttccgcgcc gtacaggccg ataccggttc tatcggcggt agcgcctctc
     5401 acgaattcca ggtgctggcg caaagcggtg aagacgatgt ggtcttctcc gacacctctg
     5461 actatgcagc gaacattgaa ctggcagaag ctatcgcgcc gaaagaaccg cgcgctgctg
     5521 ctacccagga aatgacgctg gttgatacgc cgaacgcgaa gaccatcgcg gaactggttg
     5581 aacagttcaa tctgccgatt gagaaaacgg ttaagaccct gctggttaaa gcggttgaag
     5641 gcagtagctt cccgctggtt gcgctgctgg tgcgcggtga ccacgagctg aacgaagtta
     5701 aagcagaaaa actgccgcag gttgcaagcc cgctgacttt cgcgaccgaa gaagaaattc
     5761 gtgccgtggt taaagccggc cctggctcac tgggtccggt aaacatgcca attccggtgg
     5821 tgattgaccg taccgttgcg gcgatgagtg atttcgctgc tggtgctaac atcgatggta
     5881 aacactactt cggtatcaac tgggatcgcg atgtcgctac cccggaaatt gctgatatcc
     5941 gcaacgtggt ggctggcgat ccaagcccgg atggtcaggg tacgctgctg atcaaacgtg
     6001 gtatcgaagt tggtcacatc ttccagctgg gtaccaagta ctccgaagca ctgaaagcct
     6061 ccgtacaggg tgaagatggc cgtaatcaaa tcctgacgat gggttgctac ggtatcgggg
     6121 taacgcgcgt tgtggctgcg gcaattgagc agaactatga cgaacgcggc atcgtatggc
     6181 ctgacgctat cgcgcctttc caggtggcga ttctgccgat gaacatgcac aaatccttcc
     6241 gcgtacaaga gcttgctgag aaactgtaca gcgaactgcg tgcacaaggt atcgaagtgc
     6301 tgctggatga ccgcaaagag cgtccgggcg tgatgtttgc tgatatggaa ctgatcggta
     6361 ttccgcacac tattgtgctg ggcgaccgta acctcgacaa cgatgatatc gaatataaat
     6421 atcgtcgtaa cggcgagaaa cagttaatta agactggtga catcgtcgaa tatctggtga
     6481 aacagattaa aggctgatgc cagaaagggt cctgaatttc agggcccttt tttacatgga
     6541 ttgtataaaa tggataaacc aaaggcgcat tgccgcctgc ttctcccctg ctttcttttc
     6601 ctttcggcct gtaccgttga tatcagccag ccagactcct ctgccacagc ggtagatgtc
     6661 gaggcaaaaa catgggtagt caaattccag catcaaagct ctttcaccga acaatcaata
     6721 aaagaaataa cggcacctga tctcaaaccc ggcgatttgc tgttctcctc aagccttggg
     6781 gtaacctcat ttggaatccg cgtcttcagc acttcctctg tgagtcacgt tgcaatcttt
     6841 ctgggtgata ataacgttgc agaagcgaca ggcgctggcg tccagatcgt ttcccttaaa
     6901 aaagcgatga agcacagtga taagcttttc gtcttacgag tcccggatct taccccgcaa
     6961 caagccacag atatcaccgc gtttgctaat aaaatcaaag atagcggtta taactatcgc
     7021 ggcattgtcg aatttattcc ctttatggtg actcgccagg tgtgctcact gaatccgttc
     7081 tccgaggatt ttcgtcaaca gtgcgtcagc ggcctggcga aagcgcagtt aagcagtgtg
     7141 ggcgaaggag acaaaaagtc gtggttttgt tcggaatttg tcactgatgc atttgccaaa
     7201 gccgggcatc cgcttacgct ggcgcaatca ggctggatta gccctgccga tctgatgcat
     7261 atgcgtactg gcgatatctc tgcgtttaag ccagaaacgc agttacagta tgttgggcac
     7321 atgaagccag ggatttatat caaggcgggt cgctttgttg ggctgactca gtgataaaag
     7381 ccggagaatg ctccggcttt caattactgc cccaaactac tgcaatcctg gttcggataa
     7441 aatttacccg ccgtatcggg tgccaatact ttagtcggcg caccggtatc cggattaccc
     7501 tcaagcgtaa agtgaccttc tactgacaat aacaccggtt tttcactgtg accgcgcgca
     7561 gccaggtagc tacgctccag ctctgcgtta ttcgctacca tgaaacgttt tccggtcgcg
     7621 caatcagtga aggtcgccgc atcagccata taaaaataca tgccccgcag ggtcatcggc
     7681 gtcataggta aactggattg tgccggttct agcgtatagt tgaactgcga ttcaatcgga
     7741 ttgccttcac gatcgagcat ctccagcgca tcgcctttcg cccgataata tgacttttca
     7801 cctttgctgt cggttaatac tagcttgtca gcggttcgcg cccatgtacc gtaggaagcg
     7861 aaggaggaag gttcttcacg agcaccgaga taacgctcat tcatcaccca tgttccgtct
     7921 ttttcgagga acagagaggt ttcgattcct tcgcaatcag cacacggcag cacgccgcgc
     7981 caactttgcg gcatcggttt cagttcggca gcctgcgccg gagaaagcgt atcgacttcg
     8041 gcccgattat tacaacccat cagagtaaag aggctgatta cagccatcgc tgtcactatc
     8101 gcttttttca ccatccattc cttcttttta ttcccgaccg ctgcgcactt tgccgcgcat
     8161 cgccttcacg cttgattttt gtgctttcga tgccagcctg cgctctttcg atgcacgggt
     8221 ggggcgcgtg ggtcgtcggg ctttttgttc tgttgttaaa tctttaatca ctgccaccag
     8281 ccgggccaga gccgcttcgc ggttcagttc ctgactgcgg tattcctgtg ccttaatgac
     8341 aatcacgcca tcactgctga tcaagtgatg gctggcggcg agcagacgtt ctttgtaata
     8401 ctctggcagg ctggacgccc gaatgtcaaa acgcagatga atagccgttg aggtcttatt
     8461 aacatgctgc ccgcccgcgc cctgcgcacg aatggcggtg atctcaagct caccatcggg
     8521 gatagcaaca tgtcgggaaa tcacaatcat gaaggttgtt gccaggcggt taagttcact
     8581 tccagattat tcttatcatc cgataaccag atcacgccat cctgaatcgt tgcctgcagc
     8641 gtcatggtac gatcggcaaa ggcgctcact ttcgccagtt gttcatcgtc cagataccag
     8701 atggaaagat tggaaaactg cgcgcatttg ctctgatttt gctgccacca gatttgcgcc
     8761 gcccgactat tataggcaaa cagcgccact tctgcggcct gggtgcaggc tttcttaatc
     8821 cgtcgctcat ccggcagccc cagctcaatc cacaaatcaa tacccaaatg atcgttacgc
     8881 agccacgctt ccggctcatc atcggcacac aaaccacggg taaattgcag acgttcatcg
     8941 gcatatttta gccacgccag caggcgcagc atcatgcgct cctgggtttc tgaagggtgg
     9001 cgcgccagcg tcagagaggc atcgagaaac tggttgcggt cgagatcggc cacattaacc
     9061 gtcgctttat aaattgtcgc tttaagcgcc ataagaaaac tcctttcgaa tcaggaggcc
     9121 attgtaacgg aatacgtgca gttgcggcgc tgatattgca gaaatgagtg tggtatagtc
     9181 accttgcgaa acagagttta tcttcgtagg cttagacttg catccacggt taagtcagag
     9241 tgctgacagg agggcatgtg gaaaaatatt gtgagttaat acgcaagcgg tacgcggaaa
     9301 tcgccagcgg agacttagga tacgttccgg acgcgctggg ctgcgttttg aaagtgctga
     9361 atgaaatggc tgccgacgac gccctttctg aggctgtcag ggaaaaagca gcatacgctg
     9421 cggcaaactt actggtgagc gattatgtca atgaatgata cgtatcaacc aatcaattgt
     9481 gatgattacg ataatcttga gctcgcctgc cagcatcatt taatgctgac acttgagctg
     9541 aaagatggcg aaaaattgca ggcgaaggcc agcgatttag tctcccgcaa aaatgtggag
     9601 tatctggtcg tcgaggccgc tggtacaacc cgcgagctgc gtctggataa aattaccagc
     9661 tttagccacc cggaaatcgg tacggtggtg gtgagcgagt cctgattgac tttcgccgga
     9721 tgcgacgttt gaagcgtctt atccggcttt cacttaacta agcgttttct gccagacaaa
     9781 actgacgcca ttctcacctt cagccacacc ttcttgcgtc acaaataccc ctgccgccgc
     9841 aatcaacgtt tcgccataaa acagcagtgg cgtggtgtca cgtagccacg gcggcacacc
     9901 cagctcttgc cagattttct tcagcttacg tccgccgttg cgcccgataa tatgcagcaa
     9961 tcctggcgct ttgaaacgca cgctgaccgc ttcgtctgca cgcggagggc gaatatcgcc
    10021 tcccgcagta agctgtacac ttcccagtcc cgccggtaat tccagcggtt gtagccacgt
    10081 ctgccacggc acaatggttt cgctttgccc agtgacggat ttaatccacc acagttgcga
    10141 ctgatagcgg cggatttcaa acgcgcccag gcgtaaacag ggggaagcat cttcccgcgc
    10201 cagcgccact tcctgccaga tcctcaccaa cgcgtcgcgg gaaggcatcg gtgcattctt
    10261 ccctgccagc cagcggcgga taatcgccgc gcggcgggca tcactcatcg ccagcattgg
    10321 cgcaatctgc agcgtcccct gcgacgtttg acagtgtgct atatcatccg ccagcagttc
    10381 atccaacagg ctctcttgtt cagcgcaaag cgcagcgctg cgggccgttg cttcggcaaa
    10441 atgcggccaa cgctgctgca ataacggcac cacgcgcagg cgcaggaagt tacgatcgta
    10501 gctatcatcc tgattacttt cgtcttcaat ccagcgtaaa tcatactgac gcgcccactg
    10561 caccagttct ccccgcgtgc gggcgagcaa cgggcggata agccgtgttc cggcaaactc
    10621 cgaaacttcc cccatagccg aaagcccggc agggccgcta ccgcgtttta gcgccagcag
    10681 aaaggtttca cattgatcgt cgagatgttg cgcggtgacc agcacttcac cgggtaacaa
    10741 ggtgcgggca aatgcctgat aacgtgcctg ccgcgcctgg gcctcaatgc ccagtccttc
    10801 ttgcgcaagt tgcacgcgct cgaccaccag cggcacctgc cactgttggc agatgttctc
    10861 gcaatgccta acccaggcat cggcattagc acttaaaccg tgatgcacat ggatagcgcg
    10921 cagagtgacg cccggatttt ccatccgcca ctgcaccaac tgatgcagca gaacggtgga
    10981 gtcaagcccg ccgctaaagg ccaccagaat ctggcgtgag gagagaagtt gtctattgag
    11041 cgtgagtgtc atgatgatgc gattttacag tagcaatgcc cggcacgtta ccgggcaaat
    11101 gcggcgatga caagccttac tgctcataca gttccaacgg cagcccgtcc ggatcgttaa
    11161 agaacgtgaa gcgtttttgc gtgtatggat cgacacggat ggcttcacac ttcacgttat
    11221 ggctttcaag gtgcgccact gccgcatcga tatcatcaac gctaaaagcc agatgacgca
    11281 ggccgcaagc ttccggtcgg ctggggcgct ccggcgggaa cggaaatgag aaaagctcaa
    11341 tcacatattg cccattaagc gccaaatccc ctttccacga gtcgcgcgct tcgcgataga
    11401 cttcgctttg cagcgtgaaa ccaagaatat cgcagtaaaa cgctttgctc accgcataat
    11461 ccgtcgcaat aatcgcaata tggtgaacct gttttaaacc cagcataacg tctcctttat
    11521 ttgttaacag cacgttactc gcccggaagc cgctctggca agttatcccg ccatttttag
    11581 gactcgtacg cggtaaacgc cgtcttcgtc ctgtttcgcg ccgtgaatat ccgtttcaaa
    11641 accggggtaa tgttgcccta cggaacaaag catcagtaga aaatcgagta ccgtgcggct
    11701 ctctttggtc agcatttctc ctggcatcag cagcggtacg ccaggtggat aaggcaggat
    11761 catatttgcc gatactctac cgaccagttg ttccagcgca atggtttcca cttcgccttt
    11821 aatctgtctc tgccatgcct gatgtggcgt catgatcatc tccggcaagg tatcaaatgc
    11881 ccgcaacatc aaaccggaaa gatcgtgttt acgaatcagc ttatggatcc cttgcgccag
    11941 atcctgaata cgcatattgc ggtagaaatc gggatcttca gcatagagat cgggtagcat
    12001 atttttgatc cgcaggttga gatcgtaaga gcgtttgaat tccgtcaacc cacgcaataa
    12061 tcccattgcc ttggttttat cgatgccaat actaaaaaga aacagcaggt tatacgggcc
    12121 ggttttctct actacgatcc cacgttcgtc gaggaatttt gccaccagcg ccgccgggat
    12181 cccctcctcg ctcatattgc cctgctcgtc catccccggt gtcagaatag tgactttaac
    12241 cggatcgaga aacatatgat cggcatccgc atcgttaaag ccgtgccatc gttcgcctgg
    12301 cgcaacgggc cagcattcgg cttcatccac ctgcggcggt tgccagatat cgaaaaacca
    12361 gccgtcagac tcttcccgca gccgctggac ctctttgcga aaatgcagcg ctcgttctac
    12421 tgaacggtta atcagccgtt tgcccggatt accacgcagc atcgccgctg ccgtctcaac
    12481 cgaagcaaca atgggataac tgggcgaggt ggtggtatgc atcataaagg cttcgttaaa
    12541 cgcctcttcg tcatactcgc ctttaatgtg gatcagcgaa gcctgcgata acgccgccag
    12601 cattttgtgg gtcgattgcg tttcgaagat cacttttccc gcaacacgct cgccgctcat
    12661 accactttta ccctggtaga tcggatgaaa atgggtgtac ggcacccagg cagaatcgaa
    12721 gtgaatcgac gggacatcca gcgtctgttt gatccagtcg gtgttgtaga gcaagccatc
    12781 ataggtggag ttggtaatca ccgcatgaac cggccattgt gcttgcgtgg tagcagcgac
    12841 tttctcttcg atgctgtcgc gagtaaattc acggcgcggg atcccaccaa gaatccccaa
    12901 cgcattacgc gtcggtttca gccagactgg cactacatcg ttcatcatca acagatgcgc
    12961 cagcgattta tgacaattgc ggtcaattaa cagcgtactg ccggatggcg cggcgtacat
    13021 acccacaatt ttgttcgatg tcgatgttcc gttggtaacg atataactct gttccgcgcc
    13081 aaaagtccgc gcgatgtact cttccgcttc caggtgtggc ccggtgtggt cgagcaacga
    13141 accaagctcg gtaaccgaaa tagagacatc agccttaaga gtattcccgc cgaaaaaatc
    13201 ataaaacaga cagccaaccg ggcttttttg atatgcggtg ccgcccatat gacccggcgt
    13261 acaaaaggtg tacttccgct ctttgacgta ggtaaacaag gctttcgtga acggcggtgt
    13321 aatgttatca agatattcgt cggtgtactg acgcatacga atggcgatat cttccgcctg
    13381 ccccagcgca tattcaaaaa accagagcgc catccgcata tcctgcacgc tgacatccat
    13441 cgtcgagtgg gtgttgatga aggcataaag cgggagatat tcattaagct gattgatatc
    13501 gctacataaa tcgagactgt actcatccca gtcaaaaatc acgccgcaaa ttcgagggtt
    13561 atgttcgata aatttcagca aatcaacgct gttttgtggc cagataatct gaaagccttg
    13621 cgccaccagc gccgactcca gttctttgat gggctcatct ttataaaaga cgccatgcgg
    13681 tcccataatg gcaatgatgt tcatgtgttc ctcctggaaa atccttcctt aatcatagcc
    13741 tgctcaaacc gtggcgataa aaaagggcca ccgaagtgac cctttttcag aacttttgcg
    13801 aattacgcgt aaccgtagct catcaggcgc tgataacgac gattttttaa atcttcagtg
    13861 cttaacacgt cgagatcggc cagatccgcc agcagttgcg ctttcaacga tgccgccatc
    13921 gcttccgggt tacgatgagc gccgcccagc ggttccggga tgatggagtc gatcagtttc
    13981 agctctttca gacgcggagc gatgataccc atcgcttcag ccgccagcgg cgctttgtcg
    14041 gcgctcttcc acagaatgga cgcacaacct tccggcgaga taacggaata ggtgctgtat
    14101 tgcagcatat tcactttatc gcccacgcca atcgccagcg caccgccaga accaccttca
    14161 ccgataaccg tacaaactac cggtacgccg aggcgagaca tttcacgcag gttgcgtgca
    14221 atggcttcag actgaccacg ctcttctgcg cccacgccag gataagcccc cggggtgtcg
    14281 ataaaggtga tgataggcat cttaaagcgt tcagccattt gcatcagacg cagtgcttta
    14341 cggtaacctt ctggcgctgg cataccaaag ttacggcgaa ttttttcttt ggtttcacga
    14401 cctttttgat gaccaatgat catcaccgga cgaccatcga gacgggcgat accaccgacg
    14461 atagctttat cgtctgcata cgcgcggtcg ccagccagtt cgtcaaattc atcaaatgcc
    14521 aggcgaacgt aatccagggt ataaggacgc tgtggatggc gtgccagttg cgcaatctgc
    14581 catgcaccga gatcggcgaa gattttacgc gtcagttcta cgcttttttc acgcagacga
    14641 tgcacttctt catcgatgtt aatatccagt ttctcatcct gacggctaac cgcagtcaga
    14701 gaatcgattt tcgcttccag ctctgcaatc ggctgttcaa aatcaaggaa attcagactc
    14761 atagtattcc tgtattagtc aaactccagt tccacctgct ccgaaccaat gaggccacgg
    14821 agatcgttta ataaacgatc gctcggagag acacgccacg tcgcgccaaa acgcaaccgc
    14881 gcgcgtgcat ccgccctctg atagtagaga tgtactggaa ttgtcccaga gcggtggggt
    14941 tccagagact gacggagtcg gtttaaaagc tggtcatcaa tttgcctgtc cgtcagcgag
    15001 atagcaagcc cgcgagcata tttttcccgg gcttcgtcaa tatccatcac ttcgcgagcg
    15061 gtcattttaa gcccaccgct gaagtcatca aagctgacct gtccgctgac gataagtatg
    15121 cggtcttttt ccagcaattg ctggtattta tccagggcgt cagtaaacaa catcacttcc
    15181 agccgcccgg aacggtcatc cagcgtgcag ataccgatac gattgccgcg cttggtgacc
    15241 ataacccgcg cggcaacaac gagccccgca gccgtgatga ctttaccacg ttctgtcggg
    15301 tgcatgtctt tcagccttac gcctccgaca taacgctcaa tctcttttag atactggttg
    15361 atagggtgtc ccgtcaggta cagacctaac gtttcacgtt ccccatctaa taccacctgc
    15421 tccggccacg gttggcagct ggcgtaggat tgttcaattt gttccggttc ttcggccagc
    15481 acgccgaaca tatcggcctg accgatagct tccgctttcg cgtgttgatc tgccgctttt
    15541 aacgcatcgc ccagcgagtt catcagcgcc gcacggtgtg gcccaagacg gtcaaacgcc
    15601 ccggacatga tcagtttttc cagcacccgc cggtttaact ttttggtgtc ggttcgggcg
    15661 cagagatcaa acagttcgcg gaagtagccg cctttattac gggcttcgat gatggcctca
    15721 atcggacctt cacctacccc tttgatcgcg ccaataccat acacgatttc gccgtcgtcg
    15781 ttgacgtgga aatggtaaag accggagttt atatctggtg gcaggatttt cagccccatc
    15841 cgccagcact catccaccag gcccaccacc ttctcggtat tgtccatatc ggcggtcatt
    15901 accgccgcca taaactctgc cggatagtgc gctttcagcc ataacgtttg atatgacacc
    15961 aaagcatagg ccgcagagtg cgatttgtta aatccgtaac ccgcgaattt ctccaccagg
    16021 tcgaagattt tcatcgccag ttcggcgttg attccgttct tttctgcacc ttcagcaaat
    16081 acagaacgct gcttagccat ctcttccggc tttttcttac ccatcgcacg acgcagcata
    16141 tctgcgccac cgagggtata accagaaagc acctgggcaa tctgcatgac ctgttcctga
    16201 tacaggatga tgccgtaggt tggctccagt accggtttca ggctttcatg ctgccactgc
    16261 acgtccggat aggagatctc ttcgcgacca tgtttacggt cgataaagtt atccaccatc
    16321 cctgattgca acggaccggg gcggaacagt gccaccagtg cgatcatatc ttcgaagcag
    16381 tcaggttgca gacgcttgat caggtccttc atgccgcgcg attcaagctg gaataccgca
    16441 gtggtttccg agcgttgcag catgtcgaag cttttcttat catccagcgg gatcgcggcg
    16501 atatccagcg gcggctcgcc attcttcgcc cgccgcttgt tgatcatctc cagcgcccag
    16561 ttgatgatgg tgagcgtacg caaaccaagg aagtcgaact tcaccagtcc ggcgtattca
    16621 acgtcgcttt tatcaaactg ggtgaccgga tgtttgccct cttcatcgca gtaaagcggc
    16681 gcaaaatcgg taattttggt cggcgcgata accaccccac cggcgtgctt accggcgtta
    16741 cgggtgaccc cttccagttt gcgcgccatg tcgatcagcg ccttaacttc ttcatccgct
    16801 tcgtagattt ccggcagctg cggctcggct tcaaacgctt tcgccagcgt catccccgga
    16861 tcgggcggga tcagtttcga gatacgatcg acaaagccgt acggatgccc cagcacgcga
    16921 cctacgtcgc ggatcaccgc tttcgccgcc atcgtaccga aggtgatgat ctgcgatacc
    16981 gcatcacgac cgtacatgtc cgctacgtgc tcgataacct ggtcgcgttt ctccatacag
    17041 aagtcaacgt cgaagtcagg catggagaca cgttccgggt taaggaaacg ttcgaacagc
    17101 aggtcaaatt ccagcggatc gaggtcggtg attttcagcg cgtaggccac cagtgaaccc
    17161 gcaccggagc cacggcctgg ccctactggt acgccgttat ctttcgacca ctggataaat
    17221 tccataacga tgaggaagta acccgggaag cccatctggt tgataacctg aagttcagtc
    17281 tccagacgtt cgtcatattc cgggcggcgc ttaacacgtt cttcctcgtc agggaataaa
    17341 aaggccagac gctcttccag cccctctttt gcacgcttga ccagataatc ttcggtgctc
    17401 atgtccccgg tcgggaactg cggcaggaag tattcaccaa gacgcacggt tacgttacaa
    17461 cgtttggcga tctcaacggt gttggcaagg gcttcgggaa tgtcggcaaa aagctcgcac
    17521 atctcctctt cgctacgcat atattgctgc ggcgaatagt tacgcgggcg tttaggatcg
    17581 tcgagggtaa agccgtcgtg gatcgcgacg cggatttcgt gtgcgtcaaa gtcgctgctg
    17641 tcgataaagc gcacgtcgtt ggtcgccaca acgggcaaac cgcgtgcttc cgccagttcc
    17701 accgcagcgt gcagatagct ttcttcgtcc ggcctgccgg tgcggatcag ctcgagaaaa
    17761 tagcgatccg ggaagtgttc ttcataaaac gcgacacact catctaccag cgcgctgtta
    17821 ccacgcaaaa gactgcgtcc gacgtcgccc atgcgcccgc cggaaagaag gatcaaccct
    17881 tcgtttaatt cgataagcca gtcgcgatcg atgatcggcc cagctgcacc gtacccgcgc
    17941 tgatacgctt ttgagatcag caacgtcaga ttctgatagc cggtattgtt cgccgccagc
    18001 accgtcaggt gggttaactc atcacccagc aggtcgcact ggacattaaa atctgccccg
    18061 acgataggct taatccctgc gccatgtccc gctccgtaga acttcaccag accgcaaagg
    18121 ttggtgaaat cggtgatcgc cagtgctggc atacccaacg ccgccgcctt ttttaccagc
    18181 ggtgcggttt tggccagacc atcgatcatc gagtagtcgc tgtgcacccg caggtgtacg
    18241 aaacgtggtt cagacatctt cagattccgg tttacttaat ctcgacacaa gaatcaggac
    18301 gcaagtccca gtacgcgttt gacaggccca aagctgcgac gatgatgttc agtcgcgcca
    18361 tattcagcca gtttttccag atgaaaagcg gttggatacc ctttatgttg ggcaaaacca
    18421 tattgcggga aaacaatatc cagcgccgcc atttcggcgt cacgcgtcac tttcgccagg
    18481 atagacgcgg cactgatttc cggtacgcgg ctatcgcctt tcaccacagc catcgcaggc
    18541 atcggtaatt tcgggcagcg gttaccatca atcaacacat attccggcgc aatatgcagc
    18601 ccagcgacgg cacgctgcat cgccagcatg gtcgcgtgta aaatattcag ttcgtcgatt
    18661 tcgtgaggtt ccgctcggcc cagactccag ctcaacgctt tctctttgat ctcttcgcag
    18721 agcgccagac ggcgtttttc gctcagcttt ttggaatcat tcagcccggc aatcgggcgc
    18781 gccgggtcga ggatcaccgc agcggtgacg accgcgccaa ctaacggccc gcgtccgact
    18841 tcatccacac ccgcaaccag gtgcgtgtgc ggataaacaa attcgatcat tgtgctaact
    18901 ccagaacggc ttgtgccgcc tgctcatcgg cattgcagcg gatctgctga tgcagttcac
    18961 ggaaggtatc gtgcatcgcg tggctggttt tcccgttcgc caacagcggt aacagcgccg
    19021 cagccagttt ttgcggctca cactcttcct gcaataattc tttaactaac tctctgcccg
    19081 ccagcaggtt tggcagcgag acataatcgg ttttcaccag ccgcttcgcc aaccagaagg
    19141 tgaaaggctt catgcgatac cccaccacca tcgggcattt cgccagcatg cactccagtg
    19201 cagccgtacc cgacgccaat agcgccgcat cgctggcgac catcgcctca cggcccattc
    19261 catccagcat atgaaccgaa aggtctggcg cgacttcagc tttgatgcgt tcaaattgct
    19321 cacggcgttt ggcatttacc agcggcacca cgatctccag atcaggatac gtctggcgca
    19381 aaagctgggc cgttttcagg aaatcggcgc taagcatctc cacttccgcg ccacggcttc
    19441 ccggcaacaa tgccagacag tgggcatcgt gagggatccc cagcacatca cgcgcggcgt
    19501 ttttatctgg atctaacggc atggcatcag ccatggtgtg accgataaaa cggcacggca
    19561 cgttatattt gtcataaaac gctttttcga aaggcagaaa tgcgagcacc agatcggtgg
    19621 ctctgcctat tttgaaaacg cgtttctgtc gccacgccca gacggacgga ctgacataat
    19681 gaatggtttt gataccctgc tttttaaggt taccttcaag ggtaatattg aagtcaggcg
    19741 catcaatacc gacaaaaaca tctggcttca gttcgccaaa acgcgttgtc agatcggcac
    19801 gaatatgcag taagcgacgc agacgaccga gcacttcaac aatgcccatc accgccagtt
    19861 cttccatttc gtaccaggct tcgcagcctt cagcctgcat tcgtggcccg gcaacaccaa
    19921 caaagcgggc gttgggcaca cgttctttca gagcgcggat taaaccggcc cccaggatat
    19981 cgccggaggt ttctccggcg accagggcaa tcgttaatgg acgctgttca gtcattaacg
    20041 aatcagaccg cgcgttgagc gtgcaaagaa atcggtaaag gctttcactt ccggatatgt
    20101 ttccgccagt tcagcaattt ccggtttcac ttcatcgagc gttttaccgc tacgataaat
    20161 cagcttatac gcattgcgga tagcggtaat cgcctcacgg ctgaatccgc ggcgcttcag
    20221 cccttcgata ttgacaccga acggcgttgc gtggttaccc tgcgcaatga cataaggagg
    20281 gacgtcctgc gccacaccgg agcagccgcc aaccatcacg tgcgcaccaa tgatgcagaa
    20341 ctgatggact gcggtcatgc cgccgatgat cgcgaagtcg tcaaccgata cgtgacccgc
    20401 cagcgttgcg ttgttggcga gaatacagcg gttacctacc gtacaatcgt gcgcaatgtg
    20461 cgcgttgatc atcagtaagt tgtcgctgcc caccttcgtc aatccaccgc cctggactgt
    20521 gccacgatga atggtgacgc tttcgcgaat gcggttacga tcgccgattt ccacacgggt
    20581 cggttcgcca gcatatttaa gatcctggtt gacttcgccg atggaggcga actgataaat
    20641 ctcattgtcg cggccaattt tagtatgacc attcacgaca acgtgagatt tcagtacggt
    20701 accctcacca atttcgacat ggggtccaac gatacaaaaa ggaccaatgt gagcgttcgc
    20761 gccaatcgac gcgccctctt ccacaatggc ggttggatgc acaaaggcgg atttatcaat
    20821 cacgtatcag gcctcccggc tacgagcaca catcatcgtt gcttcgcaaa ctactttacc
    20881 gtcgaccaga gcaacccctt taaaacgggt caggccgcgg cgcgtttttt cgaaagtgac
    20941 ttccatgatc atttgatcgc caggcacgac cgggcgctta aagcgcgctt cgtcaatacc
    21001 ggcgaagtag tacagctcac ccggttccag ttttcctacg cttttaaacg ccagaatacc
    21061 tgttgcctgt gccattgctt ccagaatcag cacacccggg aaaatcggtt ttccagggaa
    21121 atggccctgg aagaatggct cattgacaga gacatttttt actgcgcgca gaaaacgacc
    21181 ttcttcaaaa tccagcacgc gatccaccag taagaacggg aaacggtgcg gcagaagttc
    21241 taaaatctct tcaatctgca gagtatgagt gttagtagtc aagatactct tcctgtcaaa
    21301 atataagaaa cgacaataat aacacggcct gccgcaatcg taagaatgag acaggccgta
    21361 aagtttggcg aacaaaagat ggaacgttag tcttgttgat taaccttgcg ctcaagcgat
    21421 ttcagacgct tgctcatgtc atcaatgttc atcaccagtg cagcggtttt gcgccagact
    21481 ttgttgggtt gcagcggaat gcctgaggaa tagacgcctg gttcagtgat gggacgcatc
    21541 accataccca tgcccgtaac cgtcactttg tcgcatattt ccatatgccc gttgattacg
    21601 ctggctccgc cgatcatgca gtaacgacca attttcaggc tgcccgccat aatgacgcca
    21661 ccggcaactg ccgtattgtc gccaatcacg acgttatgtg caatctggca ctggttatca
    21721 atgatcacac cattgccaat aacagtgtca tccagcgcgc cacgatcaat ggtagtgcag
    21781 gcaccgatct ccacgcgatc gccaataatt acgcgaccaa tctgtgggat cttcacccag
    21841 ttaccacgat cgttggcata accaaagccg tctgcgccta ccactgttcc ggactggatc
    21901 aggcaattct gaccgatctg gatctcatgg taaatggtta cgttcgccca gagacgcgaa
    21961 cctgcaccga ttttgctgtt tttacctacg aagcaaccgg caccgataat cacgttatcg
    22021 cccagttcaa cgccggactc aatcaccgcg ttagcgccaa tcgatacgtt gttacccagc
    22081 ttcgccgtcg cgtcaatcac cgcactgggt gcaatgttct gcgcgggctg cggcgtggta
    22141 tctaaaattt gcgccatgcg cgcgtaagtc aggtagggat tcttcactac cagcgcggca
    22201 cttttcgcga aaggaagatc gtcctgggtc atgacaaccg cggacgcctg gcacaagcct
    22261 aaatgctcac ggtattttgg gttaaccatg aacgtgatgt gacctgtttg tgcagattgc
    22321 atggacgcaa cgccggtgat gacgatatcg ccatcaccgt gtagttctgc atccaactgc
    22381 tgcgctaaat cagccagtcg aattgaaggc attacttatt taacctgttt cagtacgtcg
    22441 gcagtgatgt cttttacatc gctgctgttg taagcaacgg cgtttgcatc aacaaccaga
    22501 tcgatatcct ggctgttggc aacggatttc acagcagtct ggatacgagt aaccagtttg
    22561 ccgcgttctt cgttggaacg acgtgcgcga tcctgctcaa aagcctgcgc tttctgagca
    22621 aaagtctggc gctgggccat cacgtctttt tccagtttgg tgcgatcgct gcccgctttc
    22681 atggactgca gctttttcat tttagcctgc agatcggttt ccatacgctg cagttcgctg
    22741 gcacggcctt tgaactcatt ttccagcgtg ttagaaactc cggttttctg cgctacctgc
    22801 tggaacaggc tgcccatatt gacgattgca attttgtcag ccgcctgagc agaagttgcc
    22861 agtgctaaac cgagacctgc agctaataac cactttttca caataaactc cttaccatcc
    22921 catttgcacc ggagggtgca gttctttgcg tggcccggcg atcttatatt gatcgcctaa
    22981 agtcatcgct acactaccac tacattcctt tgtggagaac acttaccagg ttttaccgat
    23041 gttaaactgg aactgttctg ccttgtctcc atcgtacttt ttgaacggct gggcgtagga
    23101 gaacaccaac ggccccaatg gggacatcca ttgtaatgcg atacccgcag acatacggat
    23161 attgcttgga tcactatagt ccggatatcc agaatattgg ctggaatccc agtttgtatc
    23221 ccaaacggta cccatatccc agaagaagga agtacgaacc gagttagcat acttatcgct
    23281 aataaacggc gtcggggtga tgaactcgag gctggcaacc gccatggcgt taccgcctac
    23341 agcatcatcc gatttacaca gatctttcgc gccgtcctga gtcgcacatt cgtaatcata
    23401 gtccggatca taattactgg cctgatgcgg gaagtaaact gctttcggac caatggtatt
    23461 ggactggaag ccacgcacgg tgctggaacc accggcatag aagttctcat agaacggcat
    23521 ctctttgccg cctaaaccat caccataacc ccagcgggta cgacccagaa caacccattt
    23581 gtgatcgtca tcaatcggca cataagtcgc cgtgtctaac gtcactttgt agtattcgtt
    23641 atccgatccc ggaatggtca ctttaccggt caggttgaca cgtgaaccat ctgtcgggaa
    23701 gtaaccacgg tcaagcttgt tataggtcca accatagttg aacgtgaagt cgtccgtttt
    23761 gaagctgtta tcctgatcag aggtgctcgg atgttcaccc atagagtaca gataacgcca
    23821 catcgcaacc tgaggctgca tgttggacag ggagttatgt acataaccca gacctgcacg
    23881 cagcgagtta tattcgttga tcgggaagcc caacgtcacg tctgtaccat aactcttgtt
    23941 ggtatagtcg gacaggtcgg cgtcatctgc ctggaagtca ttatagaaga gacgaccacc
    24001 gaggcttaca ccatctacgg tgaagtacgg gttggttacc gacagttcag cataggtctg
    24061 gtaatcgttt ttggtcccgt tgataccaac agcataaccc gtacctaacc agttatcctg
    24121 ttgcacacca gcctggaagc tcacgccact ttcagtaccg taaccaatcc cgaagttgaa
    24181 gctaccggtg ttacgttctt tcaccttgta gactacatca acctggtccg ggctacccgg
    24241 aacgcgttgg gtatcggtat cgacagtttc aaagaagccc agacgattca gacgctcctt
    24301 accctgatcg accagatcgc tccccagcca tgcaccttcc atctgacgca tttcgcgacg
    24361 caggacggca tctttcgagg tatcgttacc ttcaaaacgg atcttacgca cgtagaaacg
    24421 gttacccgca tcaacgttca cacgtaattt aacggttttg tcggcatcgt taatttcagg
    24481 catcgactgt acgcgcggat aggcataacc atagcgacca agaagctttt taatgtcatc
    24541 ttccattttg gtcactttgg tgccgttata cagctcgccc ggctcgatct tagtcagctg
    24601 ctcaatttca gcggagtgcc cggcaaggtt gccgctcact tcaacgccag aaagcttgta
    24661 ctgatcgcct tcggtgatgt tcaccgtgac gtaaatacct tttttatcag gcgtcagact
    24721 gacctgggta gagtcgatgt tgaaacgggc ataaccgcga tccagatagt aactgcgcag
    24781 ggtttcaagg tcgcccgcca gtttctgttt ctggtattta cgatcgccta ccacgttcca
    24841 ccacggcact tcgtcacgca gttggaaatg agagatcagt tcgtcggtgg tgaaagcatg
    24901 gttaccaaca atgttaattt gctggatttc agctgacaca ccttcctgga acaccagttt
    24961 taggtcaaca cggttgcgcg gcagcggggt cacgacagct ttaacgctgg cgctatattt
    25021 accgacgctg tagtagaagt cttccagacc tttctcgata tcggcaatgg tggtgcgatc
    25081 gagggattcg cccacacgca cgccagaagc ctcgaggttt tgcttcagca tgtcatcttt
    25141 caccgatttg ttaccggaga aagtaatgct ggcaatggtc ggacgttctt ttacctgaac
    25201 cagaagggta tcaccatcac gaaggacgcg aacatcctca aagttgccgg tagcaaacag
    25261 agcgcgaatg gtattactga tatcttcatc attaaccgtg tcgcctgtgc gcaccggcat
    25321 actgaggagg gccgcaccaa cggcgacacg ctgaaggcct tcgaaatgaa tatctttcac
    25381 tacgaaccct tcagcaccgt atacggtggc gctgctaaac agcagcgacg ctatgagcaa
    25441 ctttttcatc gccatcgtta ttatgcgttc ttcctaacta actctcataa ccgagagaaa
    25501 tcattgaaaa gtgcaagccc cattaacagc accagcaaaa tcgagccaat gcgataacaa
    25561 aagtcttgaa cccgctcgga taccggtccg cccttgatct tttcgatcgc aaggaacagc
    25621 agatgccccc cgtcaagtac gggcaacgga aacaggttaa ttatccctaa gttcacgcta
    25681 ataagcgcaa gaaacggcag gtaataaaca accccgagtt ccgctgtcat cccagccccc
    25741 ttggcgatag agatcggccc actgaggttg ttcagtttca catcaccggt gatcaatttt
    25801 cccagcatac tgaccgtcag cttcatcagc tgccacgttt tgtccgtggc ttcgacgatg
    25861 gcgttgaacg gcccatactg gcgtacaact ttatactcat ctggcaaagg aatgactttc
    25921 ggctcaatac cgacaaaacc aatcgcttta ccattacccg gtttactctc cgggattaat
    25981 gtcaaagaca agggactccc ctgcctttcg atttctaacg ctaaggattt acccgggtta
    26041 tcccggacaa gcatcacaaa ggtcacccac tgcgttaagg gctgaccatc gactttaacg
    26101 atcctgtcgc ctgcttgcaa acctgccttg cttgccgccg agtttggctg cacattttcc
    26161 agtacaggtt caatttgcgg cccacgagga cgaatcccca gcgaagttac cggatcttct
    26221 ttatcaggct caaacgccca gtgacgtaaa tcgagcttta catcccgccg ttggtcgctg
    26281 ccaaatggcg ctaccgtaat ggtggtgctt tcatcgccaa ttttatcgac caactgcaaa
    26341 cgcacggcat cccaatcagg cgtttcgata ccatctacgg cttttagttc cgtacctggt
    26401 gcaatttgtg cttccgcagc tatcgaattg gctgctattt cgccaaccac cggacgtacg
    26461 ccaggcacac caataataaa aaccagccag taggcaaaga tagcaaaaat gaagtttgca
    26521 accggacctg cggcaataat cgccgctcgt tggccgacag atttattatt gaaggcatgg
    26581 tggcggagtt ccggaacgac cggttctgcg cgctcatcca gcattttgac ataaccgccc
    26641 agcgggatca gggcgataac atattcggtg ccgagcttat cagttcgccg ccagagcgcc
    26701 ttaccaaacc ctattgagaa acgctcaacg cgaacaccac aacgccgggc aacccagaaa
    26761 tgaccaaatt catgcacggt gataagtaca cccagtgcaa cgatgaacga agccaaatcc
    26821 cagagaaaac tcagcatatt accttccgtt aaagcgtcct gaataccagt aacaacaagc
    26881 aagcaaagac cggtaccgca gccgtcaggc tatcaatacg atctaaaata ccaccgtgtc
    26941 ctggaattaa atgaccgctg tccttaattc ctgcttcacg cttaaacata ctctcggtca
    27001 gatcgccgag cactgaggct aacgctgcga caatagagca aatgagtaag gtgacgggag
    27061 cgacgtcgag attcgcccac atgccataac cccatgagat taccgctgcg gtcgcgagtc
    27121 cacctataaa gccttgccag gttttacccg gagaaacctt cggtgccagc ttatgtttac
    27181 caaacaattt gccaaacata tatgcgccag agtcagcgcc ccataccagg atcatgacat
    27241 agagcagcca tattgcgcca ctgtaatgat tctcgtcgta gtgccaggcc cgtaacgcca
    27301 gcatgcccca gaagaaggga acaatggtta gcacgccaaa aataaggcgc aatgttttag
    27361 agttacgcca gattgctgcg gaaccagggt aaaacagcac cagcaatagc gcgacaatcc
    27421 accaacccag cgaagcccaa agtgagattt caaccagcgg ttgatgaata tttcggtgat
    27481 attccggcaa cagaaaaagc atcagcgcca acaataaccc gcataacacc gccaaccata
    27541 ctcgctgcga acgagtggta aaaccgctaa gctgtcccca ttcccacgct gccagcatgc
    27601 agaccaccag cgttacaatg gcgaacccca ccggcggcaa cagaaacaac gccgcgatga
    27661 caacgggtat taacacaaaa gcagatatca ggcgatactt cagcaaaagc gacccccatc
    27721 aggctgtttc atcaccgggc tcggtgccgc cgaaacgacg ctctcgatta gcaaaggcat
    27781 ttaacgcccc ttcaaagtct tgttcatcga aatcgggcca gagaacatct gtaaagtaaa
    27841 gttcggcata ggcaatttgc caaagcaaaa agttactaat gcgatgctcc cccccagtcc
    27901 taattactaa atctacaggg gccagttcat gcatacagac atgctggttt agcatctctt
    27961 catctatctg atctggttgc aggtttcctt gctgcacctt ttcagccagt tgcctgactc
    28021 cctggactat atcccaacgt ccaccgtagt tcgccgcaat attcagcgtc agaccggtat
    28081 tcccggctgt tagcgcttca gatttacgaa tacgttcttg caaacgcgag ttaaagcgac
    28141 tggtatcgcc aataatacgc agacgcacgt tatgtcggtg cagacttttt acttcgctat
    28201 cgagcgccca cacaaacagt tccattaacg cactgacttc ctgcgctggt cggttccagt
    28261 tttcactact aaaggcatac agcgttaacg cctcaatacc gttgttggcc gcaaaagaga
    28321 cagcccggcg gacggatttt gccccggctt tatgcccaaa ggcacgaatc ttcccttgct
    28381 tttttgccca gcgaccattg ccgtccataa tgatcgcaac atgacgacag ccatgcgctg
    28441 gcaatttttc gctaagtggt tgagtagcag acaacatcac gcgtttctta ttccctgaca
    28501 gggtttacgg tactcaggaa tactgaagcc tatacaaaaa aaagccgtgt aaaaccacgg
    28561 catacctgac cttataaagc caactactta cgatcagatg gcgcagacta tatcactgaa
    28621 gccctacgct aacaaatagc gcgactctct gtagccggat tatcctcagc ttgcgagacg
    28681 catcacctct tttctggcga cttcacgcgc gttcgcatca acagataaca catcgtccac
    28741 acattgtggt tcgcgcatat ccattttttc cagtacggat aaattcaacg cggcgatatc
    28801 cgtaaagcgg atttgttgcg caagaaaagc agctacggtg atttcgtttg cggcattcaa
    28861 tgctgtcgtc gctgcctggc cttgttcgaa cgcctccatc gccagtttca ggcatggata
    28921 acgatcataa tccggtgcgg caaatgtcaa cgcacttagt ttgcaaaaat cgagcggctt
    28981 cacgccagag ttcacgcgat tcggccatgc catggtgtgg gcaattggcg tacgcatatc
    29041 cggttccccc agctgcgcca gaacactgcc gtcctgatag cgcaccattg agtgaatcac
    29101 tgactgcggg tgaatcagca cttccatctg gctggcgctg gcgttaaaca gccaacgcgc
    29161 ttcaatgtat tccagacctt tgttcatcat ggtagccgaa tcgacagaaa ttttacgtcc
    29221 catcgaccag ttcggatgac ggcaggcttg atcgggcgtc attgttgcca aatcgcgcaa
    29281 tggcgtctca cggaaagggc caccagaccc ggtaagtaaa atggacacca cgccattttg
    29341 ctcaaggtca gcgtatccca gattatgctg gataggttgc ggtaaactct gaaaaatggc
    29401 gttatgttcg ctatcgaccg gtaacaattg cgctttgctc tgctttacgg cgtccataaa
    29461 cagacgtccg caggtaacca gtgattcttt attggccagc aaaatggttt tacccgcgcg
    29521 gatcgcagca agcgtaggta acagcccagc agcgccaaca atggctgcca tcacctgatc
    29581 aacatcctca agcgctgcca tatcgcaagc ggcttgttgc ccacttaaga cttcggtgcg
    29641 gctaccctgt tgctgtagca tcgttttaag aagtttcgca ctcgcttcat catccattac
    29701 agcatagcgg ggagagaatt ccaggcactg ttctaccatg cgagtgacat ttttgcctgc
    29761 caccagcgca actacgcgga agtgttcggg attatggcgc accacgtcca gcgtgctgca
    29821 accaatcgag ccggtcgagc ccagaatggt gagttgcttc atgaaacatc cagagttgag
    29881 acagaataaa aagcaaaacg ccgccagccg atccgcaagg atctactgag cggcgttttt
    29941 gtcgttcaag aaatcagaac tgcatcagtt ctgcttcttt gtctgccagc gccgcttcaa
    30001 ttttcttgat tgcagcatca gtcagtttct gtacatcgtc ctgagaacgg cgatcgtcgt
    30061 cttcgctgat ctctttatct ttcaacagtg ctttcacttt gtcgttcgcg tcacgacgca
    30121 cattacgtac tgcaacacgc gcttgttctg cttcaccacg aacgattttg gtcagatctt
    30181 tacgacgttc ttccgtcagc ggcggcagcg gaacacggat gtcgctaccc gcagagttcg
    30241 ggttcaggcc aagatcggac gccataatcg ctttttcaac ggccggagac attgaacgat
    30301 caaacacgtt gattttcaga gtacgggaat cttctaccgt tacgcttgcc agctgacgca
    30361 gcggcgtcgg cgtgccgtaa tattccacga caatgccatc cagcaggctg ggagaagcac
    30421 gacccgtgcg tattttgctg atttgggttt tgaacgcttc tacgcattta tccatgcgta
    30481 cttcagcatc ttttctgata tcgctaatca cgttacgaat ccttgaaaac ttgtctcagt
    30541 cagaccagac agtcacacac agtggaagtg tgctaagtat agccctgatt aaacatatta
    30601 cgggtaatcg tctggattat taggctattt tatttgccat tttggccccg ggcagtgctc
    30661 gccatcctca cgtacttttg tacgctccgg ttgctgcgcg ctggccgtga ccaaactgcc
    30721 tgcaacaata acgccttata accagactta cgcccccgcg atacttacgc ggaatcttac
    30781 ccttatttat ccatcacggg aattattccg tgattaaagt cccttctttt tcacccatta
    30841 ccacacggcg cagcgcaccc ggtttgttca tgttgaaaac acgaatcggt aatttatggt
    30901 cacgagcaag cgtgaaggcc gccaggtcca tgactttcag ctctttttcc agcacttcgc
    30961 tgtaagtcag ttgctcgtac atggttgcgg ttggatcttt cgccggatca gcagtaaaca
    31021 cgccgtcaac tttggttgct ttcagcacca catcggcttc aatttcgata ccacgcaggc
    31081 aagctgctga gtcggtggta aagaacgggt tacctgtacc ggcggagagg atcaccacac
    31141 ggttgttgcg caacaggctg atagcttctg cccagctgta gctgtcgcac acgccattca
    31201 atggaatagc ggacatcaga cgagcgttca cataggcgcg gtgcagtgca tcacgcattg
    31261 ccaggccgtt cattacggtc gccagcatcc ccatgtggtc gcccacaacg cggttcatac
    31321 ccgctttcgc cagaccagcg ccacggaaca ggttaccccc accaatcacc acaccaacct
    31381 gaatacccag ttcaaccagt tctttgattt cctgagccat gcgatccagt atgcttgcat
    31441 caataccgaa gccttcagtg ccctgcagag cttcgccact caacttaagc agaatgcgtt
    31501 tatagacggg ttttgcattg gtagccatgt ttctttcctg agactgtcaa cgattgaaat
    31561 gaattaatac tagcgacatt gtacgtcgct tttcagcgca gccccacaag ggttagctga
    31621 atttacaaga tgggcacaaa aagaagccgc cctcaggcgg ctccttttcg ataattaaga
    31681 ctgcttggac atcgcagcaa cttctgctgc aaagtcagtc tcaactttct cgatgccttc
    31741 acccacttcg aagcggatga agccagtcac ttcagcgtta tgctctttca gcagctgacc
    31801 aacagttttg cttggttcca taacgaacgg ctgaccggtc agagaaactt cgccggtgaa
    31861 tttcttcatg cggccttcaa ccattttctc tgcgatttct ttcggcttac cagactgcat
    31921 cgcgatatcc agctgtacct ggtattcttt ttctaccact tcagcggata cgtcttctgg
    31981 tttgatgaat tctggcttgc ttgcagcaac gtgcatagcg atgtgtttaa ccagctcttc
    32041 gtcagcgcct ttagcagcaa ccagaacacc gatacgcgca ccgtgctgat aagaacccag
    32101 aacgtcgcct tccagcgcag caacgcggcg aatgttgatg ttttcaccaa ttttcgctac
    32161 cagcgcaaca cgttcttctt cgaactgtgc tttcagtact tcaacgtcag tgattttgcc
    32221 agcaacagct gcgtccagaa ctttgtctgc gaacgcctgg aaaccagcgt cttttgcaac
    32281 gaagtcagtc tggcagttaa cttccagaat gatgccgtag ttgccgtcga ttttggtttt
    32341 gatcacgccg tcagcagcaa cgttgcctgc ttttttcgct gctttaatag caccggactt
    32401 acgcatgttt tcgattgcca gctcgatgtc gccgttagct tcagtcagtg cttttttgca
    32461 atccatcatg cctgcgccag tacgctcacg cagctctttt accagggatg cggtaatttc
    32521 agccattcta aaatcctcgg agatgtgatc tgcccggcat taaaccgcac agatataaaa
    32581 gtgaaaaagg gggccatatg caggccccct aaccaaacgt gtactacctg gtctataagg
    32641 gctctccccc ttcatatttc gcgccgcaga tgcgttatct tcgctcgctc atcccggtca
    32701 cttactgatg taagctcccg ggaattctcg agcttgccgc ctttctgcaa ctcgaactat
    32761 tttgggggag ttatcaagcc ttattactca gcttctacga agctttcttc cgcctgggaa
    32821 gccagatcct gagaacggcc ttcacgtacg gttgcagcaa cagcgcccag gtacagggtc
    32881 acagcacgga ttgcgtcgtc gttacccggg ataacgaagt caacaccgtc cggatcagag
    32941 ttggtatcaa cgatagcaaa taccggaata cccaggttgt ttgcttcttt gatagcaatg
    33001 tgttcgtggt cagcatcgat tacaaacaga gcgtccggca gaccgcccat gtctttgata
    33061 ccgcccaggc tgttttccag tttctccagc tcacgagtgc gcatcagcgc ttctttcttg
    33121 gtcagcttgt cgaaagtacc gtcctgagac tgagtttcca ggtctttcag acgtttgatg
    33181 gactgacgaa cggttttcca gttagtcagc ataccgccca gccagcgatg gttcacgaag
    33241 aactggtcgc agctcagagc agcgtctttc accgcttcgc ttgcagcgcg tttagtacca
    33301 acgaaaagga ttttaccttt gcgagaagca atcttgttca gttcagccag agcttcgttg
    33361 aacatcggta cagttttctc aaggttgatg atgtgaactt tgttacgcgc accgaagatg
    33421 aacggcttca ttttcgggtt ccagtaacgg gtctggtgac cgaagtgaac accagccttg
    33481 agcatgtcgc gcatggaaac agttgccatg attaaaacct ctatataaaa gttggggtta
    33541 tgcctccacg tatcccatat taccgacccc aaagggcacc ccggaatatg tgccgatacg
    33601 tgtgtgttgt tacacaaagt gagattgtcg cttccgtcca gtctgtgtat acgaaatgga
    33661 tcggaagtcc ggcgcgcttt ataccacaaa tacgtcgtgg acaccaataa ttgttggcgc
    33721 tgtgtacagc atcagacgtt gaattttcta ttatagaaaa ccttcagtgg cacgtttggc
    33781 gaaattcaga atgattctca atttgcccgg gtgtgatacc attgacggca cttacatata
    33841 tattgtcggt atcaccgacg ctgatggaca gaattaatgg ctatctcaat caagacccca
    33901 gaagatatcg aaaaaatgcg cgtcgctggc cgactggctg ccgaagtgct ggagatgatc
    33961 gaaccgtatg ttaaaccggg cgtcagcacc ggcgagctgg atcgcatctg taatgattac
    34021 attgttaatg aacaacacgc ggtttctgcc tgcctcggct atcacggcta tccgaaatcc
    34081 gtttgcattt ctattaatga agtggtgtgc cacggtatcc cggacgacgc caagctgctg
    34141 aaagatggcg atatcgttaa cattgatgtc accgtaatca aagatggttt ccacggcgat
    34201 acctcgaaaa tgtttatcgt cggtaagccg accatcatgg gcgaacgtct gtgccgcatc
    34261 acgcaagaaa gcctgtacct ggcgctacgc atggtaaaac ctggcattaa tctgcgcgaa
    34321 atcggtgcgg caattcagaa atttgtcgaa gcagaaggct tctccgtcgt tcgtgaatat
    34381 tgtggacacg gtattggtcg cggcttccac gaagaaccgc aggtgctgca ctatgactcc
    34441 cgtgaaacca acgtcgtact gaaacctggg atgacgttca ccatcgagcc aatggtcaac
    34501 gcgggtaaaa aagagatccg caccatgaaa gatggctgga cggtaaaaac caaagatcgc
    34561 agcttgtctg cacaatatga gcatactatt gtggtgactg ataacggctg cgaaattctg
    34621 acgctacgca aggatgacac catcccggcg ataatctcgc acgacgaata agatgaagcc
    34681 ggcgaatgcc ggctttttta atgcgataat ttaatcttat gggtggcgca caatgaatac
    34741 ccttccagaa cagtacgcaa acaccgctct ccccaccctg cccggtcaac cgcaaaatcc
    34801 atgcgcctgg ccccgtgatg aattaaccgt ctgtgggata aaagcccata tcgatacttt
    34861 ccagcgttgg ctgggtgatg cctttgacaa tgggatctcc gcagaacagt tgattgaggc
    34921 acgcaccgag tttatcgacc aactcctgca acgattatgg attgaagcgg gattcagcca
    34981 gattgccgac ctggcattgg ttgccgtcgg tggttatggt cgtggcgagc tgcatccact
    35041 ttccgacatc gatttactga ttttaagccg taaaaagctc ccggatgatc aggcgcaaaa
    35101 agtgggcgag ctgttaacgc tgctctggga tgtaaagctg gaagtcggtc atagcgtgcg
    35161 cacgcttgaa gagtgcatgc tggaagggtt atcggattta accgtcgcca ccaatttaat
    35221 cgaatcccgc ttattaattg gcgatgtagc gctcttcctc gaactgcaaa aacatatttt
    35281 tagcgaagga ttctggcctt ccgacaagtt ctacgcagcg aaagttgaag aacagaacca
    35341 gcgccatcag cgttaccacg gcaccagcta caaccttgaa ccagacatca aaagtagtcc
    35401 tggcggcttg cgcgatattc acactctgca atgggtggcc cgccgtcatt ttggcgcaac
    35461 atcgctggat gaaatggtcg ggtttggctt cttaacctca gcggagcgag cggaattaaa
    35521 cgaatgtctg catatattgt ggcgtattcg ctttgccctg catctggtcg tcagccgtta
    35581 cgataatcgc ctgctgtttg atcgccagct tagcgtcgcc cagcgtctga attacagtgg
    35641 cgaaggtaac gaaccggtcg agcggatgat gaaggattac ttccgcgtta cacgccgcgt
    35701 cagtgaactc aaccagatgc tgctgcaact gttcgatgaa gccatcctcg cccttcccgc
    35761 cgacgaaaaa ccacgtccaa tcgacgatga gtttcagcta cgcggaacgc tgatcgacct
    35821 gcgtgatgaa acactattta tgcgccagcc ggaagccatc ttgcgtatgt tctacaccat
    35881 ggtgcgcaac agtgcgatca ccggcattta ctctaccacg ctgcgccagt tacgccacgc
    35941 ccgtcgccat ttgcaacaac cgctgtgtaa tattccggaa gcgcgaaaac tgtttttgag
    36001 cattctgcgt caccctggtg cggtgcgtcg cggactatta ccgatgcatc gtcatagcgt
    36061 gctcggcgcg tatatgcccc aatggtcgca tatcgtcggg cagatgcagt ttgacctgtt
    36121 ccacgcctac acggtggatg aacatactat tcgcgtgatg ctgaaactgg agagttttgc
    36181 cagtgaagaa acgcgccagc gccatccgtt gtgtgtagac gtctggccgc gcctgccgtc
    36241 aactgagctg attttcatcg ccgcgctgtt tcacgatatc gccaaagggc gtggcggcga
    36301 ccactccatt ctcggtgcac aagacgtggt gcattttgcc gaactccacg ggcttaactc
    36361 gcgcgaaaca cagctggtgg cgtggctggt tcgccagcac ttgctgatgt cggtgaccgc
    36421 ccaacgccgc gatattcagg acccggaagt gatcaagcag tttgccgaag aagtgcaaac
    36481 agaaaaccga cttcgttatc tggtatgcct gacagtggcc gatatatgcg ccaccaacga
    36541 aacgctgtgg aatagctgga agcaaagtct gttgcgcgaa ctctatttcg ccaccgaaaa
    36601 gcagctacga cgcggaatgc aaaacacgcc ggatatgcgc gaacgggttc gccatcacca
    36661 actccaggcg ctggcactac tgcgcatgga taacatcgac gaagaggcgc tgcaccaaat
    36721 ttggtcacgc tgtcgtgcta actattttgt ccgccatagc ccaaatcaac tggcctggca
    36781 tgcgcgccat ttattacagc atgatttaag caaaccgctg gtattgctta gtccgcaggc
    36841 tacgcgtgga ggcaccgaga tttttatctg gagcccggac cgcccctatc tgtttgccgc
    36901 cgtctgtgcc gaattagacc gccgcaattt aagtgttcac gacgcacaaa ttttcaccac
    36961 tcgcgacggt atggcgatgg ataccttcat cgtgctggaa cccgatggta gcccgctatc
    37021 cgcagatcgt catgaggtta ttcggtttgg tctggagcag gtgctgacgc aaagtagctg
    37081 gcagccaccg cagccccgtc gccaacccgc caaattgcgc cattttactg ttgaaaccga
    37141 agtgacgttt ttgccgaccc ataccgaccg caaatcgttc ctcgaactga tcgccctcga
    37201 ccaacctgga ctgctggcgc gagtcgggaa aatttttgcc gatctgggaa tttcgcttca
    37261 tggcgcccga attacaacca ttggcgagcg agtagaagat ttattcataa ttgccaccgc
    37321 tgaccggcgt gcgcttaata acgagttgca gcaggaagtg catcagcggt tgacagaggc
    37381 cctcaatcca aacgataaag ggtgatgtgt ttactgatat gaaaagagtt taacaatgca
    37441 gcagttacag aacattattg aaaccgcttt tgaacgccgt gccgagatca cgccagccaa
    37501 tgcagacacc gtaacccgcg aagcggtaaa tcaggtgatc gccctgctgg attccggcgc
    37561 actgcgtgta gcggaaaaaa ttgacggtca gtgggtgacg catcagtggt tgaaaaaagc
    37621 ggtgctgctc tctttccgta ttaatgataa tcaggtgatc gaaggggcag aaagccgcta
    37681 cttcgataaa gtgccgatga aattcgccga ctacgacgaa gcgcgttttc agaaagaagg
    37741 cttccgcgtt gtgccaccag cggcggtacg tcagggtgca tttattgccc gtaacaccgt
    37801 gctgatgccg tcttacgtca acatcggcgc atatgttgat gaaggcacta tggttgatac
    37861 ctgggccact gtcggttcct gtgcacaaat tggtaaaaac gtccatctgt ctggcggtgt
    37921 aggcatcggc ggcgtgctgg agccgctgca ggctaacccg accattattg aagataattg
    37981 cttcatcggt gcacgctctg aagtggttga aggggtgatt gtcgaagaag gttccgtcat
    38041 ctctatgggc gtatacattg gtcagagcac ccgtatttac gaccgtgaaa ccggcgaaat
    38101 ccactacggt cgcgttccgg ctggttcggt ggttgtttct ggcaacctgc cgtcgaaaga
    38161 tggcaaatac agcctttatt gtgcagttat cgttaagaaa gttgacgcga aaactcgcgg
    38221 caaagtcggc attaacgaac tgctgcgtac catcgactaa aaatcagtac acgggcagca
    38281 tgacgctgcc cgattttttg cagggatgga tgatgatcac acgcaggcgt tttttacagg
    38341 cagccgctgc gacgttagct gccggatcaa gcctcagcta tatgcattat tgggaacctg
    38401 gctggcttga attaatccgt caccgcctcg ccttttttaa agacaacgct gctcctttca
    38461 aaatcctttt tctggcagat ctccattact ctcgttttgt tcctttaagc ctgatttctg
    38521 acgcaattgc tcttggcgta gcacaaaagc ccgatttgat attgctgggc ggcgattacg
    38581 tattatttga tatgcagctg aatttttcgg cgtttagtga cgtactctcc tcccttgccg
    38641 aatgtgcgcc gacatttgcc tgttacggca accacgatcg ccctgttggt acagagaaaa
    38701 atcgcttaat tggcgaggca ttaaaatcgg cggggatcac ggtgttattt aacgaagcca
    38761 tgctaatcac cactcaaaag cagcaatttg aactggttgg cacgggtgat ttatggaccg
    38821 gacaatgcaa accgcctccc gccagcgaag caaatctgcc acgactggtg ctggcgcata
    38881 atcccgacag caaagaagtc atgcgtaatg aaaagtggga tctgatgctc tgtggtcaca
    38941 cccacggcgg gcagttacgc ataccgttgg ttggtgagcc ttttgcgccc atagaagata
    39001 aacgctacgc cgccggttta aatgcctttg gtgagcgaca aatttacacg actcggggtg
    39061 tcggaagttt gtatggcttg cgtctgaatt gccgcccgga agtgacaatc ctggaactgg
    39121 tgtagaaagc gaattctctt atcagaataa agcaggtaat tttttgattt ttaggccatg
    39181 ataaaagtct ggaagaaaag gcttcctgcg atctccgtcg agctaaaccg ccaggcgaaa
    39241 tgctaagata agcacaatta ctgactcttt tgaggtacaa ctatgtacga caatctgaaa
    39301 agtttgggca ttaccaatcc tgaggaaatt gatcgttaca gcctccggca ggaagccaat
    39361 aacgatattt tgaaaatcta ttttcagaaa gataaaggcg agtttttcgc caagagtgtg
    39421 aagtttaaat atccgcgtca gcgcaaaacg gtggtcgccg atggtattgg ccagggatat
    39481 aaagaagttc aggaaatcag ccctaatctt cgttatatca ttgatgagct tgatcaaatc
    39541 tgccagcgtg atcgtagcga agttgacctt aagcgtaaaa ttctcgacga cttgcgtcac
    39601 ctggagtcag tggtgaccaa taaaatcagc gagatagaag cagatttaga aaagctgaca
    39661 cgtaaataat tttgcatatt gccattctgc cagccgggta aagtatacgc tgaataattc
    39721 gagtcgccgt acatgcaact tgaattaaga cgagcatatt tcccggcatt tactattttt
    39781 accctatcgc tgttcatcca gttgtaacgc cacatacaac aataacctgt catcaaaatt
    39841 ccccagatct aagcctgtga gttccgatat acgattaagc cgatactcca gggtattacg
    39901 atgaataaac agcgcctttg acgttgccag cggctgcacg ttatggcgaa accacgccgc
    39961 cagcgtacgt cgcagcaagc cgttattatc catcgctttc agccgcgcca gcggtcgcgc
    40021 cagttcgttg gcctgccagt cgccacgcaa actgtcgagt aacacaggta acatcagatc
    40081 ctgataaaaa tagcagcgac tttctggcat ccgctgtttc cccactacca tcgtcgtttt
    40141 cgccgtacga taggatcggg caatactgcc aggaccggta aaatagttgc ccagcgaaac
    40201 gcgaaaacgc aactggccat actctttcat gcgggtaatc agttgttcaa ctctcttacg
    40261 atgatcttct gcgtcccagc gcccaaaaga gttcaacgcc ggtttcaaca ccaccatttc
    40321 ggttagcgag acaatcgcca ccagattatt acgctcgggc gtagtcagcg cattttgcag
    40381 ttgctgtaac tccgccattg cgctgtccac gccaagctga ccgctgtcga cctcaacaat
    40441 agccaccact cgcggttgat tgagatcgat ccccagccgt tgcgcccatt cagtaagtgc
    40501 gggagtattc tcctcagcct gaatgaggtt catcaccagt tcttcccgca aacggctatc
    40561 ctgcgccaac aggtgcatca accgcgactg ttccagcatc atttcagccg tcatgcagac
    40621 cagttcgcca tatttacgca gattctctgg ttcacctgtc aggccaatta cgccgacaat
    40681 ttcaccttcc agccgtaacg gtagattaat cccctgccgc acaccgtgca gatgacgtgc
    40741 caccgcgtca tcgatatcga cgactcgtcc ctgtgaaagt accagcaatg ctccttcgtg
    40801 caattcacca atacgctcac gatcgccgct gccgataatt cgcccgcggg catccattac
    40861 gttgatattg gtatcgatga tgcgcatggt acgtgctacg atatcctgcg ccattttggt
    40921 atcaagatgc cagccagcca taagtcctcc gttatgcacg gcttagcata aggaagtacg
    40981 taacgtacgg cattgtgcaa atgcccaaag gatgatgaag atgtatggag ttgtggcgaa
    41041 gttcacagat tgtaaggaga accccttccc gtattcagga aggggttgag ggagattact
    41101 gcattaacag gtagatggtg ctgtcgccgc gctgaatgtt cagtgccagc acagacggtt
    41161 tgctgtcgag aactttacgc agttcagcga tgtttttcac tgcctgctgg ttcgcgccaa
    41221 taatcacatc accttttttc aggccgatct gcgcagccgg agtgcccgtt ttcacgttgt
    41281 tcactaccac gccctgatct ttgcctttgt tgctcatctc agcgccttca atgccgttga
    41341 agatggagct ggaatcaacc tgattctggc tgctctgctg cagttccagg ttcacgttaa
    41401 cctgcttacc gtcgcgcagt aagcccaggg tcagtttgct gcccaccggc atagtaccca
    41461 cctgagcacg cagggcggca aagctgctga ttggcttacc gttcagtgag gtgatcacat
    41521 cacccgcttt aatgcccgct tttgcagcgg aggagttagg cagaacctgg cttacgaaag
    41581 caccgcgctg ggcgtcaact ttcatcgctt tcgccagttc ggagttcagc tcagtcccca
    41641 taatacccag ctcaccgcgt ttcacctggc cgtattccac catctgcgag gtcaggtttt
    41701 tcaccatgtt actcgggata gcaaaaccga taccgatgtt gccgccgtcc ggtgcgagga
    41761 tcgcggtgtt gataccgatc agttcgccgt tcaggttaac caacgcacca ccggagttac
    41821 cacggttgat cgctgcatcg gtctggatga agttttcgta gttttcagca ttcaggccgc
    41881 tacgccccag cgcagagaca atcccggaag ttaccgtctc gcccagacca aacgggttac
    41941 caattgctac ggtgtaatca cccacgcgca gtgcatcaga atccgccatc ttaattgcgg
    42001 tcagattttt cgggttctgg atctggatca gcgcgatatc agagcgcgga tctttgccaa
    42061 ccatcttcgc gtcgaactta cggccatcgc tcagctggac tttaatcacc gtcgcgttat
    42121 caacaacgtg gttgttggtg acgacatagc ctttatcggc atcaatgatg acgccggaac
    42181 ccagcgccat gaatttctgt tgctggccgc caccattacc gccctggccc ccctggcaga
    42241 acggagagct ctggaacgga gaaccttcct ggcagaacgg agaatcatca ccgaagaact
    42301 gctggaaatt acgcggcata cgcggcgtat taacggttgt gctaccttct acgttaatgc
    42361 tgaccactga aggcatcacc ttttcgagca tcggtgcaag gcttggcatc tgctgggctg
    42421 tcgttgctga agaagtctca gccgccgttg cagagagcgg agataacgcc aaacctaaac
    42481 tcagagccag tgcactcagt gctaatgtgg tttttttcat gtatttcagt ctcgattaac
    42541 agataacgca aaattgctgt gttcttcaga ttcgttttat agcgcgaagt tccgaactaa
    42601 agtttctgca aaaggtaaaa atttattgtt cgtctttaca aaagcctggt tattgttcta
    42661 cggccatcag acgtcggtat tcatcccacg catagaggtc ggtcataccg ctgatataat
    42721 cctgcagcag gcggcaacgg taataatatt cccatagcgg aaactcagga gaatctgacg
    42781 gtaatttact gacagcctcg acataggcca gccgatggcg cgtcgagagt ttgttgaata
    42841 agcgcgattc aatagggaaa cgtttcaccc gttctttttc taccagttca gtaaagtctg
    42901 ataacgacag gcttaataaa gggcgataaa tctctaataa tccgctaatg acccgatagc
    42961 cctgcaattc aagctgctcg acatctggat ggctaaatac atgttttaca gcgacatttt
    43021 tatatagctt tagaagatca ctgcattcgc tggcatcttc caataatgca tgattaaacg
    43081 ttccggcgaa aatcgcaggc agattatcaa taaatcgttg tgccgcgtag ggtaccagtt
    43141 tatttagggt gtttacccgt aaatacataa aaaactgatc ttccgtactg cggcttaaac
    43201 tatttgagcg tgatttttcc caggcatttt caaccaccag cgaatagagc gaacctttct
    43261 catgctggcc ccacgcttcg tgcaaatgat gataaagctg ctcaacggta aatattcttt
    43321 tctctaccgc atcttcaagg tctgccacac aataggagat gtcgtcggca gcttccataa
    43381 tccacgttaa tggaaaacga ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa
    43441 tataggcttc ctcagaaaga taataacccg gctttttcat taaatagtga tgtgtctcag
    43501 gcgtttcacc acgccaccac gccggacgag tatattttaa aataccgcca acctgtgccc
    43561 aggtgagatt catccgcatc aatgtatgca ccagacgaat gccttgtgca ttcccctcaa
    43621 aatgacataa gtcctgacgt attttgcgcc gcagctcgtt aagcggttct tccccgtccc
    43681 gtaaacgtag tgctgccacg ctgcagcgat cgtcagtcag aggctggctt tcggcatctt
    43741 ccgggtgcaa acgctggcga aaccagtcat ttatcgccgc ttcgccaaaa tgaccaaacg
    43801 gcggattgcc gatatcgtgc ataaggcatg acatctcaac aatgctttca aaggggccgg
    43861 tcagttcatc caggccgtat gcttccagta atttaagctc tttcagacgg cttaaaattt
    43921 ctttggcgat gtagcgcccc acctgctgga cttccatcga gtgggtaaga cgcgtgcgca
    43981 cggcggcatt gcgctccagt ggaaaaacct gggttttttg ttgcagacga cgaattgccg
    44041 gagagttgat gatacgcccg cgatcgctct cgaagatccg taggatctca tgttcggttt
    44101 taacgccctg cggtgaacgg taacgacgat gccagtttat tttttttcgg aaatcaatct
    44161 gtgccatgtc ctctcccgcg tgagaaatac gcttccccgt aagcgcatgg taaactatgc
    44221 cttcaaatcg ggcttatcgc gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa
    44281 gaagaagtta cgctactgcg tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt
    44341 tgcgaaatct ataccggcca actgaatgga accgaggttg cgcttctgaa atcgggcatc
    44401 ggtaaagtcg ctgcggcgct gggtgccact ttgctgttgg aacactgcaa gccagatgtg
    44461 attattaaca ccggttctgc cggtggcctg gcaccaacac tgaaagtggg cgatatcgtt
    44521 gtctcggacg aagcgcgtta tcacgacgcg gatgtcacgg catttggtta tgaatacggc
    44581 cagttaccag gctgcccggc aggctttaaa gctgacgata aactgatcgc tgccgctgag
    44641 gcctgcattg ccgaactgaa tctaaacgct gtacgtggcc tgattgtcag cggcgacgct
    44701 ttcatcaatg gttctgttgg tctggcgaaa atccgccaca acttcccaca ggccattgct
    44761 gtagagatgg aagcgacggc aatcgcccat gtctgccaca atttcaacgt cccgtttgtt
    44821 gtcgtacgcg ccatttccga cgtggccgat caacagtctc atcttagctt cgatgagttc
    44881 ctggctgttg ccgctaaaca gtccagcctg atggttgagt cactggtgca gaaactggca
    44941 catggctaag tcactgttca gggcgctggt cgccctgtct tttctcgcgc cgctgtggct
    45001 caacgccgcg ccgcgcgtca tcacgctttc tcccgccaac actgaacttg cctttgccgc
    45061 cgggatcacg ccggttgggg tcagcagcta ttccgactat cctccacaag cgcaaaaaat
    45121 tgagcaggtt tccacctggc aggggatgaa tctggaacgc attgtggcgc tgaaacccga
    45181 tctggtgatt gcctggcgtg gaggtaatgc cgagcggcag gttgaccagc tggcttctct
    45241 gggaataaaa gtgatgtggg tcgatgcgac aagcattgaa caaattgcca atgcgttacg
    45301 tcaactggtc ccctggagtc cgcaaccgga taaggccgaa caagccgcgc aatccctgct
    45361 ggaccagtac gcgcaattga aagcgcaata tgctgataaa cctaaaaaac gtgtttttct
    45421 gcaattcggc attaatccgc catttactag tggaaaagag tcgattcaaa accaggtact
    45481 cgaagtttgt ggcggagaaa acatctttaa agacagccgg gttccctggc cgcaagttag
    45541 ccgcgaacag gtgttagcac gcgcgccaca ggcgattgtc attacaggcg gaccggacca
    45601 aattcctaaa atcaaacaat actggggtga acagctcaaa attcccgtta ttcctctcac
    45661 gagtgactgg tttgaacgtg caagcccacg tattatcctc gctgcacaac agctctgtaa
    45721 tgcgctttca caggtagatt aatgcctgac cttttcaggt gctcagtggg gattgaacaa
    45781 tgctcgtcta ttggctggat atagtcggca cagcggtatt tgctatctct ggcgttttgt
    45841 tagccggaaa attgcgtatg gacccttttg gtgttctggt attgggcgtg gttaccgcag
    45901 taggcggcgg aacaattcgc gacatggcgc tggatcacgg cccggtattt tgggtgaaag
    45961 atcccaccga tctggtcgtt gcaatggtca ccagcatgct gaccatcgtg ctggtgcgcc
    46021 agccaagacg cttaccaaaa tggatgttgc cggtgctgga cgccgttggt ctggcagtgt
    46081 ttgtcggcat tggcgtgaat aaagccttta atgcggaagc cggtccgtta atcgcggttt
    46141 gtatgggcgt cattactggc gttggcggcg ggatcattcg tgatgtactt gcccgcgaga
    46201 tccccatgat tttacgtaca gaaatctacg caacagcctg tattatcggc ggtattgtcc
    46261 acgctacggc ttattacaca ttttccgtac cactggaaac agccagtatg atgggcatgg
    46321 tcgtgacgct attgattcgg ctggcggcta ttcgttggca tcttaagcta ccgacgtttg
    46381 cgctggatga gaatgggcgt tgaagagaga agatgtgccg gatagtttat ccggcacatg
    46441 aacaacagat tagatgctaa aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt
    46501 ggtcacgatg aaacgagaac cttccagacc ttcggtatag tcaacggaac cgccgaccag
    46561 atattgcagg ctcatcggat caaccaccag gccaacgccc tgtttttcga tggtcatatc
    46621 gccttcgttc acctgatcat caaaggtgaa accatactgg aagccgctgc aaccgccacc
    46681 ggtaatatac acgcgtaatt tcagattcgg gttatcttcg tcagcgatca ggcttttaac
    46741 tttgttggct gctgcgtcgg taaactccag cggcagtgct acgtcatcac tcatattttg
    46801 ctccaaacga catcggcaat tgggcaaatt ctaacccaat aataatcgcc attatctaat
    46861 accctggtat ttcgttcaag tattctcgct ggctgatgcg gccttgcttc gcgccagttg
    46921 ctcagcttcc tgtttcgcca gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc
    46981 ggtaaattgc gctaatagtg ttgcgccaag accggtaata atcattggca aaatgagctg
    47041 gtagttatcg gtcatttcca gaaccagaat gatccccgtt aacggcgcgc gaatagatgc
    47101 cgccagtaat gcccccattc cggcaatagc aaatgtcccc gcctcaaggt gatattgcgg
    47161 aaacagctca acggcaacca ttccgaaagc ggttcccagc acagtaccca gcgccagcat
    47221 cggggcaaaa ataccgcccg gcgcgccgga agagaagcag agtaaggtgg taatgacccg
    47281 cgcgacgaag ataaacacca gcattcccat gctgaaattc cccgcggtag cgataggaat
    47341 caggttaaaa ccgccgcccg acgttgctgg tgccacaaac cccagcaatc cacacagacc
    47401 gccaattgca ccgcccatca gtacccattt ggtaatattg ccgccgtgca cacggtgcag
    47461 caaatcctgc atccccagca cccatttatt aaaaataggg ccgaaaatgc caaaaataat
    47521 accgaggatc agataaagcc acagcgtatt aagcggcgcg tcagaaagtt taccgacgtc
    47581 aatcaacgca acttcatgat taaaaatccg gtacataatg gtcgacataa tgacgccaat
    47641 aaataccgct ttaatcgaaa ttaacgtata gcgaaactgc ggacgcatct cttcgataat
    47701 aaacaaaata cccgccagcg gggcgttaaa ggccgcagcc agccccgccg cagcaccggt
    47761 tgccagcagc gtatggcgcg cttcatcacc tttcaggcgg aaaacatcaa gcaccatgcg
    47821 accgatattg ccgccgatct gtacggttgg cccttcgcgg cccagcacca tgcctccgcc
    47881 gagtgttccc agcccgccaa agaatttcac cggcaacaca cgccaccagc gaacgggacg
    47941 ttgatcttcc agcgcccctt caatttccgg gatccccgaa ccacctgctt ccggcgcgta
    48001 tttgcgcacc aaaaagtagc cgaacatcgc cagcaccgcc gaacagagaa aagcgacggt
    48061 taacagtagc ggataattat cagcagtatg taccagtgcc cccatacgtt ggttctgcaa
    48121 ccaggcgaca cctttgtcaa aagcaaccgc agccagcccg acaagcgtgc cgacgactgc
    48181 cgccataaac aaaatggcta acggggtttt atcgcgctca agaagttggc gaatcagttg
    48241 tctgcgtcgc aggcgcgcgg cctgcggtgt ttctaaagag ggagtatcag ttttcatcca
    48301 atgatcactt attggtcata caaataagat gacggcattt tactcgcacg ttccatgaaa
    48361 atcctttgca aagcgtaatg tttcaaatgc acaaaacttt tatactctaa gaaatcagga
    48421 ttgcaccagt acaagcagcc tgatgtttga cgagtattta acttgttatg aataacatag
    48481 aatagcagcc attcactttt tctacgaatc aggaaccctc catgagtaag tctgaaaatc
    48541 tttacagcgc agcgcgcgag ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta
    48601 ctggcgtggg cggcactcca ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg
    48661 ttgatggcaa agcctatatc gattatgtcg gttcctgggg gccgatggtg ctgggccata
    48721 accatccggc gatccgcaat gccgtgattg aagccgccga gcgtggttta agctttggtg
    48781 caccgaccga aatggaagtg aaaatggcgc aactggtgac cgaactggtc ccgaccatgg
    48841 atatggtgcg catggtgaac tccggcactg aagcgaccat gagcgcaatc cgcctggccc
    48901 gtggttttac cggtcgcgac aaaatcatta aatttgaagg ttgttaccac ggtcacgctg
    48961 actgcctgct ggtgaaagcc ggttctggcg cactcacgtt aggccagcca aactcgccgg
    49021 gtgttccggc agatttcgcc aaacatacct taacctgtac ttataacgat ctggcctctg
    49081 tacgcgccgc gttcgaacag tacccgcatg agattgcctg tattatcgtc gagccggtgg
    49141 caggcaatat gaactgcgtt ccaccgctgc cagagttcct gccaggtctg cgtgcgctgt
    49201 gtgacgaatt tggcgcgttg ctgatcatcg atgaagtgat gaccggtttc cgcgtagcgc
    49261 tagctggcgc acaggattat tacggcgtag tgccagattt aacctgcctc ggcaaaatca
    49321 tcggcggtgg aatgccggta ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg
    49381 ccccgactgg tccggtctat caggcgggta cgctttccgg taacccgatt gcgatggcag
    49441 ctggtttcgc ctgtctgaat gaagtcgcac agccgggcgt tcacgagacg ctggatgagc
    49501 tgacaacacg tctggcagaa ggtctgctgg aagcggcaga agaagccgga attccgctgg
    49561 tggtaaacca cgttggcggc atgttcggta ttttctttac cgacgccgag tccgtgacgt
    49621 gctatcagga tgtgatggcc tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc
    49681 tggacgaagg tgtttatctg gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc
    49741 acagcatgga agatatcaat aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt
    49801 gatgattttg cctcgcccac cgggcgaggc attctattaa cggctctgct ttctcaacaa
    49861 atagataaaa tatggcgcgc cgataaaggt tgacagcagc cccgccggga tctggaatgg
    49921 aaacaatacc atccgcccac accagtcagc gaacaccagc agtaaaccac ccaccagcgc
    49981 cgaaattacg atgtgtggca tcgtccgtcg aaaacccatc atccgcgcaa tatgcggtgc
    50041 cattaaacca acaaaactca acggtccaat agtcatcgtc gcggtcgccg tcaggcaagc
    50101 cgctaatagc agcagcgcaa ttcgcgtcgg cgtcagcgcc attcctacgg ctcgtgcggt
    50161 atcaccgccc agcggtaaaa tggtcagcca gcggcggcac agcgggttaa tcgccagcaa
    50221 aattaccatc acaattcccg tgcgccagac ctgcgcatcg gtcgcgttgt aggtcgaacc
    50281 ggagatccag gtcagcactt gcgccattcg cgggtcacca cttgcctgca acatcatcaa
    50341 aagcatggtg aacgcggtac ttaacgccat ccccgccagt aacatgcggt gtggggaaaa
    50401 tccaccgcgt ccggcggcga tcataatgat caacagcgtc accgccgcgc cgagactgcc
    50461 tgcaggtaac agccagccaa aggcattacc cggcaccaga aacagcatca acaccacgcc
    50521 aaacgccgcg ccggagctaa tccccagcac ttccgggctt gccatcgggt ttccggtcag
    50581 ccgctgaata atacagcccg ccaccgccag catgacgccc gcaaacagcg ccgccataat
    50641 tcgcggccag cgccagggca ttaaatcctc gagcaacgcc ccgctcgccc acgtccagcc
    50701 gtgcgcatca cgaccaaacg acagcgccac taccacagcc atcaacagca gcacgccgcc
    50761 cgcgagggca aacgccagca catgttggcg ttcagccgcg acacgatcgt tgaccttcat
    50821 atccggcgcg ctaatgctgc gtaaacgcgg cagcagccac agtagcagcg gcgcaccgat
    50881 caacgcagtg accgaaccgg tggaaacttc catccacacg cgagtcagcc agaggatgat
    50941 ttgatcagaa agccacagaa ttaacgcacc aatcagcgac gccagcatta accgtggcag
    51001 cagacgccgc gcccccagca tttttgccag cagcggcgcg aacaacccga taaagccgat
    51061 aatccccact gcattcacca gcagcgcact gatgactatc gccagcgaca gcgccgccag
    51121 acgcgccagc gacaaggcca gaccgagatt gcgcgccacg ccatcatcaa gccccatcag
    51181 ggttaacggg cgaagtagca ataacgtcag catcacgccg cccagcagct gcggccataa
    51241 acgctcaacg ccgccccagt cggtttgcgt cagcgttccg gtgctccaca ggaacatgct
    51301 ttgtagctgg tcgtgatgga agataaccag taactgattg attgccccac aataaaggct
    51361 cactaccagc cccgcgagga tcagcgttac cggcgacagc cgtttccccc acgcgacgcc
    51421 aaagacaatt aagccaacaa cgcaagcccc tgccagcgca gcaaactggc tcgccatagc
    51481 acctgggatc gcccagagcg tagtgacggt aatccccagt tgcgcgcctg ttgcaacgcc
    51541 caacgtcgtc ggttccgcca gcgggttacg cagcacttgc tgaaacagca cgcccaccag
    51601 ccccaggccc gcgcccacca gcagcgaaat cgccagacgc ggcaacaagc tgtagtgaaa
    51661 aatcatctgc tcgatgacgt caatatccgg cgaccaggcg gcctgcgccc actggctgcg
    51721 cggcaacgcc tgcgagaagt tcatccaggt gagcgccgta gcgacaatca ccagcagcgc
    51781 cagcaataac gccggaaaaa gcgcaattcg tttactcacg ctttacctcc gatggcgtta
    51841 tccagaatac gcacaaagtg cattgccgaa agcgtcgcgc catagaacca gaccgccgga
    51901 acgcgctgaa aacgtcctgc acggacaaac ggcatggcct gccacagcgg ggttgccatt
    51961 agcgcatcca tgtctttgct gttgtcgtga tcaaaacaga gcacatcaac gtctttatac
    52021 gccgccagac gatcgatact gacggcggta ctgccccaga agttggtttc cccttgccag
    52081 gcatttggga tgccgtactc atcaagaatt tcctggaaca agctgtttgg accgaagacc
    52141 aacatatggc gcggatcgat aagcgtcgtc agcaataacg ggcgtgcacc acgcttcaca
    52201 aagcggggtt tcatgctgcg gataaagtct tcatattgcg ctaaatgcgt ttctgctgcg
    52261 ctttgcaggt taagtaaatc tgccatttcc gtcagtgatt tacgcgccat cgccagcggc
    52321 tgtttgccgt cgctgaagtt aaatccgcgc cccggcgcaa tacgtgccag catttctggt
    52381 gaagggccat atcctgccga ccagatcata aacgacggtt tcatttcggt cagcagttca
    52441 aggttgggtt ctgtacgcaa accaacgtca atcactgagt ccggcaatgg cggttcgctg
    52501 acccacaggc gatagttgat ggtgtccgcc acgccgtaag gcacgatgcc gagcgccagc
    52561 agtaattcca ccggcaacca ctccagcgcc acaatacgat tgggatcaat aatcgccgcg
    52621 tgggcggtat tcatctgcca taacaacgga gaaagcgcca tcgccgttaa cagtcggcgg
    52681 cgcgaaataa gaggtaagcc gctcatcaat aaacaaaact cacaggtgca gcacccgccg
    52741 gatgcggcag aatacccatc gggatgccat aaatcatttc gagggtttcg ccgcgcataa
    52801 tttccgcagg cgttccctga gcaatcattt caccaccgcg cagggcgacc agataatcac
    52861 agtagcgcgc agccatatta atatcgtgca gcacggcaat gaccgtcaga ccacgttcct
    52921 gacttaaacg atgcaccagc gccagcacat caacctggtg ggcgatatcc agcgccgagg
    52981 tcggttcgtc gagcaacaga caacggctat cctgcgccac cagcatagcg atccacgccc
    53041 gctgacgttc gccgccagag agactatcga ccagccgatg cgccagcggt tttaagccaa
    53101 ccagcgaaat cgcctcttcg accttttcgc gatctgccgc accaaagcgc cccagtgcgc
    53161 catgccacgg gtaacgacca atcgccacca gttcacgcac ggtcatccct tctgccggag
    53221 gaagctgctg cggcaaataa gccactttgc gggcgaacgc tttgctgctc cagctttcca
    53281 gcggctgggc atcaagaaga atttcgcctt ccgacggtgg ctgatgacgg ccaagcattt
    53341 tgagcagagt ggatttacca gaaccgttgt gaccaatcaa accagtcact ttcccggcag
    53401 gaaaggttaa cgacagcgga tgcaaaagcg tgcgccctgg cacacgaaag gagatattac
    53461 gcagtgcaaa agtggtatcg gaatgattcg tgtattcctg cataacagcc aacttgtgaa
    53521 gcgggcacgg aaatccgtgc cccaaagaaa aaattagaaa cggaaggttg cggttgcaac
    53581 gacctgacgt tctgcgcccc agaagcagcc ataggtctgg aagcaactgg caacatactc
    53641 acgatcgagc aaattgttga cgttcacagc aaggctggat cccgccatac caaagcgtgc
    53701 cagatcatat tttacaacag catccattac agctgcgcta cccactttaa aagtgttcgc
    53761 cggatcacca tagctggaac caataaaacg accacctgtt cccaatgtta aaccagaaag
    53821 cggcccttca ttgaaggtat aatcccccca gagagatgcc atatgctccg gtacttgttc
    53881 tggagtttta cctttgagat tggtgtcttt ggtgtattcc gcatctgtgt aggtataaga
    53941 agccgtcaag ttaatattgg catttaacgc cgcttttgct tcaagttcca caccacgagc
    54001 gcgaatctca cctgctggga cttgcgctaa cgggtttgtt gggtctgctg tcaggttatt
    54061 tgttttcgtc aattgataga ctgcgcccgt aacaacgacc ggcatatcat tcggaacata
    54121 ttttacgcca gcttcatact gttcaccttt cgatggctta taggaaacgc gcgggttgct
    54181 ccacagatcg aaagcactcg gttcaaagga ctggctataa ctaaagtaag gtgaaatacc
    54241 attatcgaat aagtagttta caccaccgcg ccaggtgaac tgatgatcat tacgctcaat
    54301 ataaccttca gtcggcgtat aagagttttc acgaacagtg gtcgcttgct tagaccaatc
    54361 gtagcgtccc cccagagtga atacccattt attccattcc gcctgatcct gaacataaat
    54421 acctgtttgt ttgctttcat tcatttggta tggctctgcg ttaccaaatg caaagtattc
    54481 aggatgatat ttgttataaa gatcgatgga tggtgcagat ccaaagctgg cattgatatc
    54541 attgcgcata cgcatgaagt ctacccctgt cagcaaagta tgctcgactt cacctgtagc
    54601 aaatttactt tcaagttggg tatcaacgct aaagttttgc agacgctcat tatccaccac
    54661 tgtcccgcgg tttagggtat gaccatcgct ggcaatccct gtgccataaa cacttttctg
    54721 tgaggttttc atttcactga aacgcagatt ctgacgcacg gtgaaggtgt cattaaaacc
    54781 atgttcaaaa ctatatccca ccattttctg gttacgggag tatgtattat tcgatgcacc
    54841 ttcattgaag tcagtcggta gacgcttacc attaggcaat ggctcaacgg tcccctcttt
    54901 cggcaaccaa ccgtaataac ccgtttctgg ttcgttctga aaataggaca ggaaagtaaa
    54961 attggttttg tcgtctggac gccatgagaa tgatggagca atggtataac gctgagattc
    55021 agagtttttc tgctgttcgt ttgttgagcg tgcgaggcca gtcaaacggt aggagaactc
    55081 accgttatca tccagcgcat cactgaaatc gaatccggtc tgaaacagat tatccgtccc
    55141 cattttaaac tgaatttctt tcaggggttc agttgtcgga cgcttactca ccatagaaat
    55201 aatcccacca ggattacttt tcccatagag aacagacgtc ggcccgcgca tcagttcaac
    55261 cctctcgagc atgtaaggat caatcacagc atcgttataa aagttgccct gaagttttag
    55321 tccatcaagg taattatttt gattcagacc aacagatgag aaaccacgaa taataacaaa
    55381 atcatatgta ttagaagcac cacgactgct aaccgtaacg ccaggtgtat aacctaatgc
    55441 ttcttttaca gactgaaatt gatgcatctg catctcttca tttgtcacaa ccgaaataga
    55501 ctgcggcgtt ttttcaattg gtgtatccgt tttagtcgca gtagcagagt gcttcgccgc
    55561 aatagttgca gccggtcccc atgcgctttc ttgcggcgca ggtgcagcgg taacggtgat
    55621 agtgtcttct ttcggttcaa ccgctgcctg tgcataaaca gacatgccgc taaccgctgt
    55681 ggctactaca actgcgattt tacgcagtga gtgttttggc tgagcagttt tggaacgcgc
    55741 cattggtata tctctgatat aaagtgaatg ataacgtaaa cgagaatgat tattataaag
    55801 ataagataat attcgaaacg ctggcgggtt cgcaagcggt acgcaatctg agctttcctg
    55861 aagggttaat aaacaaccag atgaaaagaa agggttaata ctttgggtgg gatgttattt
    55921 taccggatgg ctactcgcca tccggtatgt cacgcttaga cgttaattac taccaaacat
    55981 atccttgatc caaccggcta caccgtcgct gtctttctgc tcttgctgag caggttgctg
    56041 ttgcggctgt tgctgcggct gagaagactg atcaaacgga ttgcctgacg gctgctgctg
    56101 catctcactc tgctggcaca gcgattgcgg atcgctggtc cagaccggca agacacgcat
    56161 gccaccgctg caaacaaagt tgccgtcata gtccacgccc atatctgcaa tatcttctgg
    56221 cggaacaaga ttcagcggtg ttggcgtctg gttagccaga taacgctgat aaatcgacat
    56281 tgccccgctg gctccataca atttggtcgg ttggttgtta tcacggccta cccaggtgat
    56341 ggtcaccgta ctgccgtcaa tgcccgcaaa ccaggtatct acgttattgt tggtagtccc
    56401 tgttttccct gccagatgca ggttcgggta tttcgcccca agctgacgcc ccgtaccacg
    56461 ttgtaccacc tgctgcatgg tccatagtgt cagatacgcc gcctgcgccg gaacagcgcg
    56521 ttccgcctgc gggaagctct gatacagcac cttgccatct tccgcgatta ccgaacgcag
    56581 tgcagaaagc ggagcacggt taccaccgct ggcgatggtc tggaacgcct gcgccacttc
    56641 gattggcgtt aagttcaacg cccccaacaa cattgctgga actggattca gttgatcttt
    56701 cggcacaccc agtttaatcc aggtctccgt aaccgcaggc agccccaacg ccatgcccag
    56761 attcaccgtc ggcacgttca tcgaacgggt caacgcatct accagcatca ctctgccgct
    56821 ttcgctataa cgacggtcat cgttctgcgg tgaccagacc tggccattcg gctgacgtag
    56881 cgcgattggc gcatccgcaa tccacgtgtt caggcgatag attttcggct ggcttaaggc
    56941 cgtcagataa gtcgctggtt ttgcaaggga accaatcgaa cgacgcgcct gcatcgcacg
    57001 gttgtagccc gcaaactgcg gctcagaacc tccaaccatc gcacgaactt caccactaaa
    57061 gcggtcgacg accacaatcg cggtttcaag atcgctcaac ttacgctgtt tcttcagtgc
    57121 cggaatgcct tccacggcgg ctttttctgc cgcgtcctgg gccaccgagt caaaggtagt
    57181 gaagatcttc acgccggaga gatcttttac cttatcgccc agttttgcct gcagctcctg
    57241 acgcaccagt tgcataaagg ctggctgtgg agagatcacc ccaccgcgcg gctgaacccc
    57301 cagcggacgt gcactcaaca tgtcatagag ttcttgatca ataatctgtt gctgttgcag
    57361 cagacgcagt accagattac gtcgctccaa cgccagtttc gggttacgcc acggattgta
    57421 gatggacgcc cctttcacca tacccaccaa cagcgcctgc tggtcgaggc tcaattcttc
    57481 tactgggcga ccaaagtaat acaagctcgc cagcgggaag ccgcggattt cgttgtcgcc
    57541 gctctgaccg agatacacct cgttcatata cagctcaaga atacggtctt tgctgtaacg
    57601 cgcgtccatg atcagcgcca tgtaagcttc gttcgcttta cgccagtaag agcgctcgct
    57661 ggagaggaac aggtttttca ccagctgttg cgtcagcgta ctcgcaccct gcaccgtgcg
    57721 tccggcggtc aggtttgcca gcaccgcacg tccgatggag tagagactga tcccatcatg
    57781 ctcgtagaaa tggcggtctt ctgtcgccag caaagtatcc accagcaaat ccgggaaacc
    57841 actgcgcggc acaaacaaac gctgctcacc gtttggcgag gagatcatgg tgatcaggcg
    57901 cggatcaaga cggaagaaac caaactgacg gttgttctcc atattgacga tcgtcgccag
    57961 atgatcgcca tcaaaggtca gacgcgcacg cacctgccct tctttactgt ccgggaaatc
    58021 aaacggacgg cggatcatct caatgctgtt ggcctgcacg gtaaattcgc caggacgggt
    58081 cattttcgac acctgacgat actgggtcgc ctccagcagc ttcaccattt cgttcttgct
    58141 gatggtcatg tctggctcaa ggttgaccat tcggccataa actgccgcgg gcaattgcca
    58201 gaccttgcca tcaatacggc tgcgaatttt ttgatcgaga taaacgccgt aaatggcgat
    58261 cagcacggca aaaacgatag ccagttttag cagcagccat agccagccgc gtttgccacg
    58321 aggcttacgc cctttgcctt tgcctttgcc cttacctttg cgcggcatcg gttcttcatc
    58381 ctcatagtca tcataatcgt cgtaatcgtc atcttcgtaa cgacgacggc ttaccttttg
    58441 tttgaccgga cgcgtcggtt tccctttgcg tccaattggc tcgcggtcat tcccggccat
    58501 gctttttctc cgcaatattc aggcgcaaaa gcccgatttt ctgttcttct gttgcaagac
    58561 agaagaagat atctctcaaa tttgctttgg tcttcacgaa tactttttcg tccgtcgcgt
    58621 cggtgcagta tttgccgggt cgtccggcca gacatgtttg ggataacgcc ctttcatctc
    58681 tttttgcacc tcacggtacg ctcctttcca gaaggcgctc aaatctcgtg tgatttgtaa
    58741 tggcctttgg gcaggggaaa gcaactccag caccagcggc acgcgcccct gggcgatcgt
    58801 cggattggtg gcctcaccaa acatctcctg cattctcacc gccagcgcgg gcggtttatc
    58861 ttcatgataa cgaatggcga tccggcttcc cgtcggcaca gtgtaatgcg caggcaattc
    58921 actatccaga cgttgctgca ttccccaatc aagtaatccg cgtagcgcct gataaatgtc
    58981 gagtgatttc aggccgcgta gtgaatgcac gccgctcata tgcggcagta gccacgtttc
    59041 cagcgtcgcc aacaaactct catcatcaac cgctggccag tcatattccg gcaaccactt
    59101 tgcggcgaat aacaaacgca agcgtagctg ttccgcttcc gccgtccagt tgagcacgct
    59161 taaaccttta tcacggatgc cgttaagcat cgcctgatgc aactcgtctt ccgagggttt
    59221 cgccagtggc tgcactttca ccgtcaactg accgatttgc agccgacgcc aggctttcag
    59281 cgtgccttgc gcgtcatccc attccacggt gtccgactgc tgtaccagct gcggacagcg
    59341 ttgtactaat tcatcgatat cgaccggtag cgccagtaaa atccgcgcat ccggcgaggc
    59401 gctgccctgc aataataacg gtgcgatcaa ccattcgtag cggcttagcg catcgtcggc
    59461 atcgagcatc gcccccatgc cgtttgccag ttgatagcgt ccatcttgcc cacggcgaca
    59521 agcaatgcga tcggcatacg ccccggcaag tagcggcgcg ataagcgaac tgtctgcctc
    59581 accgccgcgt acgtttaagc gttttaacag ttgctgacta cgttgctgcc aggcgggttg
    59641 attgcgcgaa aacgccacgc ccaggtcact attgcccatc cgtggcggct cttcgagaat
    59701 ggcggcaatt tttgccgcgg tagcagcttc gtcgtcgttc ttcgcgctaa ccagcattgc
    59761 cgctaaacgc ggatcgctac ccagcactgc cattttttgc ccttgtgcgc taagccgttc
    59821 accctccagc gcccccagca tttgtaacag ccgtttcgcg gcctgtagat tcaccgttgg
    59881 cggttgatcc agccagctca tctgcgccgt atcgctgcat ccccattgca gtaattccat
    59941 cagcaaaccg gaaagatcgc tttgtaagat ctccggttca ctttgcgcgg cagcgcgttc
    60001 tgcttgttct ttggcgatta aatgcaggca gatacccggc tccagacgcc cggcacgccc
    60061 ggcacgttgc gtcatcgacg cctgactaac gcgttgagta accagccgcg taagccctgt
    60121 acgcggatca aaatgcgcca cacgctcctg ggcacaatcc accaccagac gaataccttc
    60181 gatggttaaa ctggtttcag caatattggt cgccagcacc actttacgca tcccttgcgg
    60241 tgccgggagg atcgcttttc gctgatcgtt cagcgacaac gcgccataca gcgggcagag
    60301 caacacatca ctgccgatgc gtgaagccag ttgttcctgc acacgctgaa tttctccgac
    60361 gccaggtaaa aataacagta atgatccact ttcctgacgc agcatttcgg cggtggcaac
    60421 cgcgacggct tcgtcaaaac gctgatgagc gggcagcggc aaataacggc gttcaaccgg
    60481 aaacgagcgc ccttctgaga tgacgacggg cgcttctggc agcatttgct gcaagcggtc
    60541 gttgtccagc gtagccgaca taatcagcag tttaaggtca tcacgcagac cttgttgcac
    60601 atcgagtaac agcgccaacg ccaaatccgc ctgcaagctg cgctcatgaa attcatcaag
    60661 gatcaccaat ccaacaccgc tcagttccgg gtcacgctgg atcatccgcg tcagcacgcc
    60721 ttcggtaacc acttccaggc gggtattcgg cccgacgcag ttttgcgcac gcatccggta
    60781 gccaacggta tcgcctggct tttcgttaag cagctccgcc agtcgttgcg caacgttacg
    60841 cgccgccaga cgacgcggct ccagcaggat aattttcccg ttaatgccgg gatgcgccag
    60901 cagttgcagc ggcagccagg ttgatttccc ggccccggtc ggcgcactta ataacacctg
    60961 cgatgcacaa tcgagagcag caagtaactc aggtaagacg gcagcaacgg gcaacgacga
    61021 cacaaaacgc tccagagggt taacattctt cgcgccacat tgtagcatcg cggtaattca
    61081 taaccgagtg cctcacatgt ctgaaccgca acggctgttc tttgctatcg acttacctgc
    61141 agaaatccgc gaacagatta tccactggcg cgccacacac ttcccacctg acgcgggacg
    61201 tccggtcgcc gccgataatt tgcatctgac tctggcattt ttaggcgaag tgagcgccga
    61261 gaaagagaag gcgctttctc ttttagccgg gcggattcgt caaccgggtt tcacactcac
    61321 gcttgatgac gccggtcaat ggctgcgttc gcgtgtggtg tggttaggga tgcgtcagcc
    61381 accacgcggc ttaatccagc tggcgaatat gctccgttca caggctgccc gcagcggttg
    61441 ttttcaaagc aatcgtccgt ttcatccaca tattacctta ttgcgcgacg ccagcgaggc
    61501 ggtgacaatc ccgccgccag gttttaactg gtcgtatacg gtgacggagt tcacccttta
    61561 cgcctcctcg tttgcccgtg gacgcacacg ctacacgccg ctaaaacgct gggcgctaac
    61621 gcaataacaa ggattgtcgt aatggaattt tctccccctc tacagcgcgc gacgctaatt
    61681 cagcgttaca aacgtttttt agccgatgtg atcacacccg atggtcgcga attaacgcta
    61741 cactgcccga atacgggtgc gatgaccagt tgtgcaacgc ctggcgatac cgtctggtat
    61801 tcgacttcag acaacaccaa acggaaatac ccacacacct gggaattgac tcaaagccag
    61861 agcggcgcat ttatttgcgt caacacgctt tgggctaaca ggttgacgaa agaggctatc
    61921 cttaatgaat caatttcaga gctgtcaggc tatagctcgc tgaaaagcga agtaaaatac
    61981 ggcgccgaac gcagccgtat tgactttatg ttgcaggcgg attcgcgtcc agactgctat
    62041 attgaagtga aatcggttac gttagcggag aacgaacagg gatattttcc cgatgcggtc
    62101 actgaacgag gtcagaaaca ccttcgggag ttgatgagcg tagcggctga aggccagcgt
    62161 gcggttatct ttttcgccgt gctgcattca gccattacac ggttttcacc cgcgcgccac
    62221 atcgatgaga aatacgcgca actattgtca gaagctcaac agaggggggt agaaattctg
    62281 gcttacaaag cggaactttc tgctgaaggc atggctctta aaaaatcact gccggttaca
    62341 ttgtagtaaa gtatgtaact ggttaattta cattctggtc gcgtgcgcaa atacgctttt
    62401 cctcacacag ttgtcaagtg ttacgtttag ataattgcta tccggaaaag catctgctat
    62461 ttatagcggc ctcatttttc ccccgaacat ggggatcgat agtgcgtgtt aaggagaagc
    62521 aacatgcaag aagggcaaaa ccgtaaaaca tcgtccctga gtattctcgc catcgctggg
    62581 gtggaaccat atcaggagaa gccgggcgaa gagtatatga atgaagccca gctggcgcac
    62641 ttccgtcgta ttctggaagc atggcgtaat caactcaggg atgaagtcga tcgcaccgtt
    62701 acacatatgc aggatgaagc agccaacttc ccggacccgg tagaccgtgc agcccaggaa
    62761 gaagagttca gcctcgaact gcgtaaccgc gatcgcgagc gtaagctgat caaaaagatc
    62821 gagaagacgc tgaaaaaagt ggaagacgaa gatttcggct actgcgaatc ctgcggtgtt
    62881 gaaattggta ttcgccgtct ggaagcgcgc ccgacagccg atctgtgcat cgactgcaaa
    62941 acgctggctg aaattcgcga aaaacaaatg gctggctaaa tacagccgtt ccatcacgtt
    63001 taccacaggc gggaaatgct cccgccttat tttttgttca acgagatgac agacacacac
    63061 tatattggcc gctttgcccc ctctccttcc ggcgagcttc attttggctc tctgattgcc
    63121 gcgcttggca gctatttaca ggctcgcgcc cggcaaggtc gctggctggt acgcattgaa
    63181 gatatcgacc cgcctcgtga agttcccggt gccgcagaga ctatcctgcg ccagctggaa
    63241 cattacggtc tgcactggga cggcgacgtt ctctggcaat cgcaacgtca cgatgcctat
    63301 cgcgaagcac tcgcctggtt acatgagcaa ggactaagtt attactgcac ctgcacgcgc
    63361 gcgcgtattc aaagcattgg cggtatttac gacggtcatt gccgggaatt gcatcatgga
    63421 ccagacaacg ccgcagtacg tatccgccag cagcatccgg tcacgctatt tactgatcag
    63481 ctgcgcggca ttattcacgc caacgaaaaa ctggcgcggg aagattttat tattcatcgc
    63541 cgtgatggtt tgtttgccta taacctggcg gtggtggtgg atgatcactt ccagggcgtg
    63601 agcgaaattg tccgcggggc agatttgatc gaaccgacag tgcggcaaat ctcgctgtac
    63661 cagctttttg gctggaaagt gccagattac attcatttac cgctggcgct taacacacaa
    63721 ggcgctaaac tttccaagca gaatcatgcg cctgcgttgc cgaaaggcga tccgcgcccg
    63781 gtactaatcg cggcacttca atttctgggt cagcaggcag aagcacactg gcaggatttc
    63841 agcgtcgagc aaatccttca gtcagccgtc aaaaactggc ggcttaccgc cgtgcctgag
    63901 tcggcaattg taaattcaac attctcaaat gcgtcatgct gagctatgat tagccgctat
    63961 ttttttgtcc tgaatgatgt ttgacactac cgaggtgtac tatttttacc cgagtcgcta
    64021 atttttgccg caaggtgcta agccgcgagg aaagcgaggc tgaacaggca gtcgcccgtc
    64081 cacaggtgac ggtgatcccg cgtgagcagc atgctatttc ccgcaaagat atcagtgaaa
    64141 atgccctgaa ggtaatgtac aggctcaata aagcgggata cgaagcctgg ctggttggcg
    64201 gtggcgtgcg cgacctgtta cttggcaaaa agccgaaaga ttttgacgta accactaacg
    64261 ccacgcctga acaggtacgt aaactgttcc gtaactgccg cctggtgggt cgccgtttcc
    64321 gtctggctca tgtgatgttt ggcccggaga ttatcgaagt tgcgaccttc cgtggacatc
    64381 acgaaggtaa cgtcagcgac cgcacgacct cccaacgcgg gcaaaacggc atgttgctgc
    64441 gcgacaacat tttcggctcc atcgaagaag acgcccagcg ccgcgatttc actatcaaca
    64501 gcctgtatta cagcgtggca gattttaccg tccgtgatta cgttggcggc atgaaggatc
    64561 tgaaggacgg cgttatccgt ctgattggta acccggaaac gcgctaccgt gaagatccgg
    64621 tacggatgct gcgcgcggta cgttttgccg ccaaattggg tatgcgtatc agtcctgaaa
    64681 ccgcagaacc gatccctcgc ctggctaccc tgctgaacga tattccaccg gcacgtctgt
    64741 ttgaagaatc tttgaaactg ctgcaagcgg gctacggtta cgaaacctat aagctgctgt
    64801 gtgaatatca cctgttccag ccgctgttcc cgaccattac ccgctacttc acggaaaatg
    64861 gcgacagccc gatggagcgg atcattgaac aggtgctgaa gaataccgat acgcgtatcc
    64921 ataacgatat gcgcgtgaac ccggcgttcc tgtttgccgc catgttctgg tacccactgc
    64981 tggagacggc acagaagatc gcccaggaaa gcggcctgac ctatcacgac gctttcgcgc
    65041 tggcgatgaa cgacgtgctg gacgaagcct gtcgttcact ggcgatcccg aaacgtctga
    65101 cgacgttaac ccgcgatatc tggcagttgc agttgcgtat gtcccgacgt cagggtaaac
    65161 gcgcctggaa actgctggag catcctaagt tccgtgcggc atacgacctg ttggccttac
    65221 gagccgaagt tgagcgtaac gctgaactgc aacgtctggt gaaatggtgg ggtgagttcc
    65281 aggtttccgc gccaccagac caaaaaggga tgcttaacga actggatgaa gaaccgtcac
    65341 cgcgtcgccg tactcgtcgt ccacgcaaac gcgcaccgcg tcgtgagggt accgcatgac
    65401 agtggcgtat attgccatag gcagcaatct ggcctctccg ctggagcagg tcaatgctgc
    65461 cctgaaagca ttaggcgata ttccagaaag ccgcattctt gctgtttctt cgttttaccg
    65521 caccccaccg ctggggccgc aagatcaacc cgattactta aatgcagccg tcgcgctgaa
    65581 aaccactctt gcccctgaag agctactcaa tcacacacag cgtatcgaat tgcagcaagg
    65641 tcgcgtccgt aaagctgaac gctggggacc gcgcacgctg gatctcgaca tcatgctgtt
    65701 tggtaatgaa gtaataaata ccgaacgtct gaccgttccg cactacgata tgaaaaatcg
    65761 cggctttatg ctgtggccac tgtttgaaat tgcgccggaa ttgctatttc ctgatggctt
    65821 atcattagtt gaagctctac aggcaaaagg ctttaatgaa ttagataagt ggtaattatt
    65881 attcgtgtga atataaatat attctgaaaa caacctcaag ttattgccaa aacaatatca
    65941 ttaagataaa taaaatttta gaaaatataa tataccttcc cgtaattctc acgccataca
    66001 ccatccctct tagctttcaa atttaatttt actaactttt atttttagta aaattaaatt
    66061 gcatatgcta tcacattatg caaaatagcc atcccgatga tgaatgaatg taatagttgg
    66121 taaacaacag acaaacatca ttaacacagt aacgattttg ttacgcagca ttgcagcaga
    66181 ctcttatcgt catgcggtgc ggttatctct ctatggaata ttattcaagg atttttatgt
    66241 ctaaaaaatt aggttttgcg ctgtctggta ttatgttggc aatggctgct ggtactgcat
    66301 ttgctgctaa cgatatggac ggtggtcagt taaatattag cggcctggtc gttgataaca
    66361 cctgtgaagc tcgtgttgat ggcggtaaca aagatggttt gatcctgctg caaaccgcta
    66421 ctgttgctga aatcaccgat ggtgtactgg ataccaccgt aggtgctaaa gctaagccat
    66481 tcagcattac tattgactgc agcaaagcta acccggctcc gggtactact gcaaaaatga
    66541 ctttcggttc cgtgttcttt agcaatagca aaggtactct gaataacgat atgtctatca
    66601 ataatccatc tgatggtgtg aatattgccc tgcataatat tgaaggttcc actattaagc
    66661 aggttcaggt taataacccg ggtgatgttt acagcaaaac tctggatgca acgtctaaaa
    66721 ctgcaaccta cgattttaaa gcatcctatg ttcgtgctga tgcaagcaaa gcagcaactg
    66781 ccggttatgt aaaaactaac tcagcatata ccattactta ccagtaaata tcagccacct
    66841 aaattgattg ggtgaatata tatataggga ataacatcaa acatatttat ttttgaacgt
    66901 tattccctgt tttaattaac tctgtgagga acggtttatg ttttttaata ctaaacatac
    66961 cgcagcattg tgcttagcga gctgtatggc ttttagctca tcagctattg cagacatcgt
    67021 aatttcaggt acacgcgtag tttataaaag cgaacaaaaa agcgtcaatg tgcgtctgga
    67081 aaataaaggc aataacccat tgctggttca gagttggtta gatactggag atgacaacgc
    67141 tgaaccaggc agtattactg tcccctttac cgctacgcca ccagtatcac gtattgatgc
    67201 taaacgtggc caaacaatca aactgatgta caccgccagt agtgctctgc caaaagacag
    67261 agaaagtgtg ttctggttta acgtactgga agttccacca aaacccgatg cagcaaaagc
    67321 agcaaatcaa agcctgctac aactggcatt tcgtacacgt ataaaactgt tctatcgccc
    67381 ggaaggattg agtggaattc catctgatgc gccattggct ctgaagtggt cctgggcaac
    67441 atctgaaggc aaagcggcat tacgcgttga taaccctacg ccctactatg tctcttttag
    67501 cagtggtgat ttagaagcca acggaaaacg ctatcccctg gatgtgaaaa tgattgcacc
    67561 gttcagtaac gacgtcatca aggtaaatgg gatgagtggc agaacaagct ctgcaaaagt
    67621 gcattttttc gccattaatg attttggcgg ctctattgaa ggcaacgcca acctttaatc
    67681 tggcaaggat ataccacagc aggaagcaga acgtgactat taaatccact aaaaaatatc
    67741 ataatcacct tacccgcatc gccacgtttt gcgcgctgct gtatagcaac agtgctttat
    67801 gcgctgaact cgtcgaatat gaccatacat ttctgatggg aaaggatgct tcaaatatcg
    67861 accttagtcg gtatacagaa ggcaacccga cactaccagg catttacgat gtcagtgtgt
    67921 atgttaacga tcaacctatc atgagccaaa gcattgcttt tactgttatt gaaggtaaaa
    67981 agaacgctca ggcctgcatt acgcagaaaa atttattgca atttcatatc agttcaccag
    68041 acaagaatag cgaaaaagcc attttgctta agcgagacga agatcttggt gattgcctta
    68101 atctggctga aatgatccct caatcatcca ttcgttacga tgttaacgat caacgcctgg
    68161 atatcgacgt cccccaggca tggattatga agaattatca aaactatgtt gatccctctt
    68221 tgtgggaaaa tggcattaat gctgccatgc tttcctacaa cctcaacgga tatcacagtg
    68281 aaagtccagg cagaacaaat gacagtatct acgcggcatt caacggtggg ataaacttag
    68341 gtgcctggcg gttacgtgcc tcgggtaact acaactggat gaccaatgtc catagcgatt
    68401 acgattttca gaatcgctat ttacaacgcg atcttgcgtc actacgttca caactggtta
    68461 ttggtgaatc ttacacaacc ggcgaaacct tcgattcagt cagtattcgc ggtattcgct
    68521 tatacagtga cagtcgaatg ctgcctccgg tattagccag ttttgcgcca atcattcatg
    68581 gcgtagcgaa taccaatgcc aaagtaaccg ttatgcaaaa tggttataaa atctatgaaa
    68641 cgacggtgcc gccaggggcc tttgccattg atgatcttag cccgtcaggt tatggcagtg
    68701 atctcattgt gactatcgaa gaagctgatg gcacaaaacg gacgttctcc cagccatttt
    68761 catctgttgt gcaaatgctg cgccctggcg ttggtcgctg ggatattagc gccggtcagg
    68821 ttttaaaaga cagtatccag gatgagccta atttatttca agccagctac tactatggtt
    68881 tgaataacta cctgacagga tataccggta ttcagctaac tgataataac tataccgccg
    68941 gattgctggg gcttggtatg aatacccccg tgggggcttt ttctgtcgat gttactcact
    69001 caaatgtcag cattccggac gataaaacct atcaaggaca aagctaccgt atttcctgga
    69061 acaagttatt tgaaaatacc agtacatcat taaatatcgc ggcctatcgc tactccaccc
    69121 agcattatct gggcctcaat gatgcgctga cgctgattga tgaagtagaa catcctgagc
    69181 aagatcttga gcctaaatcc atgcgcaatt actcgcgcat gaaaaatcag gtcacggtca
    69241 gtatcaacca accgctgaaa tttgagaaaa aggattacgg ttcattttat ctctcgggaa
    69301 gctggtcaga ttactgggcc tccgggcaaa acagtactaa ttactctatt ggttatagca
    69361 atagtgcgtc ctggggcagt tacagtatca gtgctcaacg ttcgttgaat gaagatggtc
    69421 aaaccgacga tagcatttat ctcagcttca cgattccaat agaaaattta ctgggtacgg
    69481 aacaccgcag ctccggcttc cagagtattg atacacaatt aaacagcgac tttaagggta
    69541 ataaccaact caatatcagc agtagtggtt acagcgatac caatcgcatc agctacagcg
    69601 taaacacggg ttatatgatg aataaatcca gtgatgacct gagttatatc ggcggttatg
    69661 ccagttatga atcgccgtgg ggaactctgt caggttcagc ctcagcaagc agtgataata
    69721 gtcgccaatt ctcactcaat actgatggtg gatttgtgtt acatagcggc gggctgacgt
    69781 tcagtaatga cagttttagt gattccgaca ctctggctgt cattcaggcg ccaggagcaa
    69841 aaggcgctcg tattaactac ggaaacagca ccgtagaccg ctggggttat ggtgtgacca
    69901 gcgccctttc gccataccat gaaaaccgta tcgcactgga cattaacgat ctggaaaacg
    69961 atgttgaatt gaaaagcacc agtactgtcg ccgtcccgcg ccagggagcc gtcgtctttg
    70021 ctgattttga aacagttcag ggacaatcgg caattatgaa tatcgtgcga agcgatggta
    70081 aaaatattcc atttgcagcg gatatttatg atgaacaaaa caatatcatc ggcaacgtag
    70141 gtcagggtgg tcaggccttt gttcgcggta tcgggcagga aggaaatata cgaattacct
    70201 ggattgaaga gggcaaacca gtcagttgtt ttgctcatta ccaacaaaac acgacatcag
    70261 aaaaaatagc gcaatctatt attctgaacg gacttcgttg tcagatacag taaccacaag
    70321 ggacttccaa tgataaaaac cacaccacat aaaatagcaa tactcatggg gctattatta
    70381 tcgccatctg tatttgcaac ggatgttaat gtcgatttta ccgctaccgt gaaagcgaca
    70441 acatgtaaca tcaccctgac atcgctgaat ggttcaaacg tcgttgataa tggcaatgat
    70501 agttataccc tgataatccc caagatggga ctggataaga tagtcaataa agcgtcacaa
    70561 tctgaggcca acttcaaatt agtcgccagt gggtgtagca gtggaattag ctggattgat
    70621 accacaatga ctggaaatca gtctggtagt acatcgctga ttattccaca atcttctgac
    70681 tcctcctcaa caaccagtaa tattggtatg ggatttaaaa gattagagac ttcgggcgat
    70741 acgtttctga aacccaatag cgcggaatac atccgctgga aaaccacaga aattagcacc
    70801 agtggtctgg aaatgactgt cgcactgcgc gaaacaaccc caggacaggg catacccggg
    70861 aaatttcgcg cacaggcaac gttcaatttc atctatcaat aacaaggatg acaatgatga
    70921 tattaaaaaa attatgttat ggattatcgg gtagtctatt tctggcttca tcactgttaa
    70981 ccatttcctc acatgcggcc actgatagta ttggcctgac tgttactaca gacatagaaa
    71041 tgggtacctg tacatcaacg ttgactgatg acggtacagc taaactgtcc accatcaatt
    71101 tcggtgatgt atatatttct gaaattaatg ccgaaactaa aattaaaacc ttcaaattgc
    71161 agttcaaaga ctgtgcgggg attcccggga aaaaggccaa aataaagttg accccacgag
    71221 ccttatgcga aggtaattct aataacggcc ctggatttgc taacgcatca acggcaacag
    71281 caaaagcagc ggcagtcgcc gttgaggtct ggagtacgtc cacaccaggt aaaaacggcg
    71341 caaaacaatt cagttgcgtc acccccgcaa cagaagaagt cagtattgct ggtgcgacgg
    71401 gaggtgatgt tgtcgattac ccgatgagtg ctgttttggt ggtcgcgaaa gataaaaccg
    71461 ttacagatgt caccgcgggt gatttcaccg ctccggcaac atttactgtg acttataact
    71521 aacaggcaga ccgattcagg aactaaatat gcgccccact caacgtaatc tgatgaaaaa
    71581 atcaattctg cttctgtctt tgctattcag caccactttc cctgtggttg caggacagga
    71641 tgttgacatt gttgcaaata taaaaaataa cacctgccaa agtggaatca gcaacaacgg
    71701 aaatattgat ttaggtgtcg ttggcgtggg cgattttacc ggaaacattt cagccgaaaa
    71761 ttatcacccc ggtggaaaag agttcactat tacagtacag gactgcacac ttcagggcac
    71821 agggaatgtg ttaaatcagt tacatattga ttttcgcgca ctcagtggcg taatggcgac
    71881 aggttccagt caaatattcg ccaatgaaga caacaccggg gctaaaaatg ttggggtagt
    71941 tatattctct attcaggacc cggcaaatac cttcaacgtt cttggcgtaa ctggtagctc
    72001 acgttccgtt tatccggtca tgtcatcagc actaaacaat tcatcctgga aatttagcac
    72061 tcgaatgcaa aaaatcgatc cagcgctgaa tgtcacctct ggccaactta taagccatgt
    72121 gttagtggat atttactacg aataacagac aaagtgctgc aagcattatc ttgcagcatg
    72181 acaatggagt aaaaatgaag atattcttca gatactttct ttttttagca ctatgctctt
    72241 gttgttatac agcaagtgcc ggcaccgatg ataacgtcag ctatcttgtt gggaatgatt
    72301 acggagtggg tccgggagac caaaagtgga gagaaacagg ccccagtgca gacattactg
    72361 tcaaattcag gtattcaaca agcacaaata acttagtttt ttataaacca acccaactcg
    72421 gaccaacagg tgtgtcatta tactggtctc agttaaatag cgcttcaggt ggaggttacc
    72481 tttactgcaa cagaagtaac actgcaagcg gtgagcctat ggcaattgaa aatcaaatgg
    72541 ttgattcggg taaacgttat ggcgggcata aactatttga aacatctgtc ccaggcctgt
    72601 attatacatt aaagatatca aacatttggt catcatacac cacaaccaat atcagttcat
    72661 caggtatgta tatcggcgat tctccaactc aatattttcg gtggatcccc gaaagtgaag
    72721 ccactctata ttggtcttgt aacaatgcaa actcatctaa taaatactgg gccgtaggtg
    72781 gagtttggca gtcgctcacg gtggaatttt ataccgatac caactttgac cccacaaaga
    72841 caaacaaggt aacattatct aagccgggtg gttatctcta ctcattcaaa ggatatggtc
    72901 ttggtggcgg tatcaacgat cacagctatt tccttaaaat agattttgac ctggataata
    72961 tgacgttatc caacccaacg tgttttactg ctgcacttac tggcccatct gtaagcggtt
    73021 caacagttaa aatgggagat tataccccag cgcaaataaa aaatggcgca acagcggttc
    73081 cttttgatat cacgctacag aactgtattc gtgttcgcaa tatagaaaca aaacttatgt
    73141 caaacaaggt gggcagtgta agcaaagaac ttctggctaa cacactcaca ggaaataacg
    73201 cagcaaaagg tgttggtgta ctgattgaag gtttaaaaaa cactaaaagt gcacaaatgg
    73261 tactgaaacc aaatgattca acgtctgttt ataaagatta cgaaacagaa gatgacacta
    73321 ccggaggaat ttacccggat aaaggaaacg gtacctcaca acctcttcat ttccaggcta
    73381 cattaaaaca agacggtaat attgctatag aaccggggga ttttaaagcc accagcactt
    73441 ttcaggttac ctacccctga taacactatt gcaccgccag acaatggcgg tgcgatacgg
    73501 ataaatcatt gcccccacca atgtgactga taaaatgcga gacagcacaa catcatttat
    73561 caggatacgc tatgaaacca accacgattg cttcattgca gaaatgcaag caggacaaaa
    73621 aacgtttcgc aaccatcacc gcttacgact atagcttcgc aaaattgttt gccgaagaag
    73681 gacttaacgt catgctggta ggcgattcac tgggcatgac ggttcagggg catgactcca
    73741 cgctaccggt taccgttgcc gatatcgcct atcacaccgc cgctgtgcgt cgcggcgcgc
    73801 caaactgcct tctgctggct gacctgccat ttatggcgta tgccacgccg gaacaagcct
    73861 ttgaaaacgc cgcaacggta atgcgtgccg gagccaacat ggtcaaaatt gaaggcggtg
    73921 agtggctggt ggaaaccgta caaatgctga ccgaacgtgc cgttccagtt tgtggtcatt
    73981 taggcctaac cccacagtca gtgaatattt tcggtggcta caaagtccag gggcgcggcg
    74041 atgaagcggg cgatcaactg ctcagcgatg cattggcatt agaagctgct ggggcacagc
    74101 tgctggtgct ggaatgcgtg ccggttgaac tggcaaaacg tattaccgaa gcactggcga
    74161 tcccggttat tggcattggc gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg
    74221 acgcgttcgg cattaccggc ggccacattc ctaaattcgc taaaaatttc ctcgccgaaa
    74281 cgggcgacat ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttacc
    74341 cgggcgaaga acacagtttc cattaaggag tgacgttgtg ttaattatcg aaaccctgcc
    74401 gctgctgcgt cagcaaattc gccgcctgcg tatggaaggt aagcgcgtgg cgctggtgcc
    74461 caccatgggt aacctgcacg atggccatat gaagctggtc gacgaagcca aagcccgcgc
    74521 cgatgtggta gtggtcagta ttttcgttaa cccgatgcag tttgaccgcc cggaagatct
    74581 ggcacgttat ccacggacct tgcaagagga ctgcgaaaag ctaaacaaac gtaaagtgga
    74641 tttagttttc gccccttcgg taaaagagat ctacccgaac ggtacagaaa cccacaccta
    74701 tgttgatgtt cctggtcttt ccaccatgct ggaaggtgcc agccgtccgg gacattttcg
    74761 cggcgtttcg acgattgtca gcaagctgtt caacctggtc cagccggaca tcgcctgctt
    74821 cggtgaaaaa gattttcagc aactggcgct gatccgcaaa atggttgctg atatgggctt
    74881 tgatattgag attgtcggtg tgccaattat gcgcgccaaa gacggtctgg cgctgagttc
    74941 ccgtaacggt tatctgacgg cggaacaacg caaaattgcg cccggtctgt acaaagtttt
    75001 aagttcgatt gccgacaaat tgcaggctgg cgaacgggat ctcgatgaaa ttattactat
    75061 tgccgggcaa gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgtgatgc
    75121 cgacaccttg ctggaggtct ctgaaaacag caaacgtgca gtgattctgg tagccgcctg
    75181 gcttggcgat gcccgcctga tcgacaacaa aatcgtcgaa ctggcgtaat ccttatctgg
    75241 cgctacggct gatggcgcca gttattcatt gccacgttat ctgtaggcaa ttattcatat
    75301 cgtagacaac ttagttagag ttaattagct tatcaatctc tgcaagagat aaagctgtca
    75361 cctctgcaac gtcttcccga gacattcctt tactcagaag acgctgggcc agtcttacct
    75421 tctggagtcc ctctttatat cccttctgaa atcccttctg aaatcccttc tcttcaaacc
    75481 actgcgccaa cgtctgcctg gtttctctcc gtgtctttat ttccatatgt tcccttaatt
    75541 atctggtcag ttaacaataa caattatctg gctataactg ataatgcaga taacttagtt
    75601 aatcagctta tcaacctcag caagaggtaa atttgccatc tctgcaacgt cttcccgaga
    75661 catcccttta ctcagaaaac gcagggcgta ttcctgtctt acttcctgtc taacctcttg
    75721 tattacctcc tgtcttacct cctgtcttac ctcctgtctt acctcctgcc ttacctcctg
    75781 cctttcctcc tgtcttccct tctcttcaaa ccactgcgcc agcgtcatca tagactcccc
    75841 tcccgtttcc ctgtctctca gcacaccata aaacaaatcc gcttgttcag tatgaccgcg
    75901 ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc cttcgtcaat
    75961 cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat gtttttgcag
    76021 tagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg tgatggtgat
    76081 atccaccagc gggaaagggt tgttgtaaac gcgtcgcgcc agctccggcg agtaaaacat
    76141 atcaaaccag cacattgaga gcggataagg tgtggcctcg ccctgataaa acagaatcgg
    76201 caccaccagc ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg cggcaatggc
    76261 atagcgcatc atgcgaaagg tcattttctt atccggcttg ctttgatgtt caatcaccac
    76321 atgcaaataa ccgggattac cctgcatttg cacggaataa agcacgtcag tgctgtgccc
    76381 tttcaggctt tcttcaataa aactccccga ctcaagatgc agcgtgtcga ggtcacacag
    76441 ttcgcgtagt tccgctggca aatggatatc cagaaagtca cgagccgttt ccgcatgcat
    76501 taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca tcttccctgc
    76561 cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgcgccgtcg tcagccgcct
    76621 ctttattgtt taccgagcag cgttcagaga aatttcaatt ggttacaggc gttacatggc
    76681 acgcttcggc acggtacgca aactgctgat gcaaatgccg tcagatgagt agacactaaa
    76741 caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcatcgcct gcttcgttaa
    76801 cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa actccaccgc
    76861 gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga ccaggatttt
    76921 cttgacgcag ctggtattct cgaaaacgaa gccattgata tctggaatgt gaccaacggc
    76981 aagcgtttct ccacctatgc catcgcggca gaacgcggtt cgagaattat ttccgttaac
    77041 ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca tcatcgccag cttcgttact
    77101 atgccagatg aagaagctcg cacctggcga cccaacgtcg cctattttga aggcgacaat
    77161 gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cctgataatc acccctgcgg
    77221 ctggttactc accagccgcg acatcgtctc gagcgaatcc gttcttaaga tataaagtcg
    77281 ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg tcaccgccag
    77341 gtgaaatccg gcttcttttg ctgccttcac ggctttgtca ttaaatccgc caaacggata
    77401 cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac gtgcaaaatc
    77461 aaacagaata ttgtgctcac tacggctcag caatatgggt cggcgataac catccacccg
    77521 atgcaaaaaa tgggtatgtg actggaaatc aaatacatcg cgaatttcgt taagttcaga
    77581 aacgctcata aattgcagcg attttgggtc ccacttctgc gggtgacgtt tgatgcgcga
    77641 ggtaacaata aacgccgtcg ccttcatgcc atattgtttc aacacaggat acgcatagcg
    77701 gctcaccgat ttgaggccat catcaaaggt aatcaccacc gctcgcgcag ggagattgat
    77761 cttattcttc acgtaacctt ccagctgcac catactcagt gtcgtgtatc ccctgtcgcg
    77821 cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag tatggcgaaa
    77881 acgggtgttt tcttcgtcgc gcagaatatg gtgataggtt agcaccggca ggccattatc
    77941 gggttgggca tccagcgcgc tgatataggc cagtcgatcg ccaatacgga tctgatacca
    78001 ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat tgtccgccag
    78061 taccccaaac ggcgcactgc ccgcactcgg cgcgttatag accggcgtat ctttccaggt
    78121 aactaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt caactttttg
    78181 tcgcccctga accggctcga gatgaccttt atcgataaac cctttaccaa agccaaaatt
    78241 aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgcccgg cccgaatatt
    78301 ccccacggtc accatcttgt cgccaatttg cgcccagacc gcagcatttt cgatggtttg
    78361 catataacgg gcaggtaacg cggcactgac gcttgcggta aacagcatca gcaggagaat
    78421 aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt attgtagcaa
    78481 aagcgtcatc agaatcgcgg gttaatactc atacaaatta tgtttgagga cgaaaggcgg
    78541 atttttttgt tgctggtgcc aaagactact cacctccggc gcacccagtt cgacaatacg
    78601 ctcgcgaaac tctgcgctgg ataacgtttc acggtagacc agcatctgct caattaacgg
    78661 taacgtgacg ccagaaatca cttcgcaacg ggaatgttta tggcttaata gcgaagccac
    78721 gcggtacggt ggcgctccgg ctatatcggt taagaagatg acaccttcac cagagtccgc
    78781 ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaacc cacgccagaa
    78841 attcacggcc cggcactgaa gaagtgcccc atgttttttc tccagcgcat ccagtatctc
    78901 ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc tccttagccg
    78961 aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa ctcctctccc
    79021 ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg ttggatcagc
    79081 gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacaccgaa ggtagtgacc
    79141 agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat ataaacgatt
    79201 gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga gtaaaagacc
    79261 ccgcccaaat acgtaagtgg cgttaacaca aaggttggca ccaggctgat gtcatcgaac
    79321 gttttggcaa acacgccgtt cagcaaaccc gcaagggaga acaacaccgc cgtgagcacc
    79381 agcgttaaag cgacaaatac ccacgaatgc acctgaaacg gcacaaaaaa cagtgaaatt
    79441 gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac atatccggca
    79501 ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg ctgaaacttg
    79561 gcaccaaaaa atgatgacgc gacgttagcg taggcgttgg tgatgaccga catcatgatc
    79621 agccccggta caatgaactg catgtagctg aagccatgca tatcgccaat acgcgaacca
    79681 atcaggttac cgaagataat aaagtaaagg gtcatggtga tgaccggtgg caccagcgtc
    79741 tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt tagtgccacc
    79801 cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa acaaacagct
    79861 cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc tcacttaact
    79921 gcgtaaatac gctgttgatc ccctgctcac gcagaacttc aacttccagc gtcgcggtat
    79981 cgaccagtcg atactgatag ccatcgagct tcggtaacgg gcttttcggt gcgagatcga
    80041 gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaggta ttttccacca
    80101 gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct tccaggtagt
    80161 gtgtggtgag aatgatagtg gtgcctttgt cgtttaaatc cttcaaaaag ccccacattg
    80221 agcggcgaag ttcgatatcc acgcctgcgg tcggttcgtc gagaatcagt agtttaggtt
    80281 catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat aacatacgcg
    80341 cacgttcgtt gcgttttccc catagatcga gttgtttaag atacttttcg ctgcgaatgt
    80401 acgctccttt gcgctccaca ccgtagtacc ctgcctgatt caccacaatt tgctgcacgg
    80461 tttcaaacgg gttgaagtta aattcctgtg gcaccagacc caactgacgt ttagcgttta
    80521 cgacatcttt ctcgagatcg taaccaaata cgctgacccg cccggaggtt ttatttacca
    80581 gagagctgat aataccgata gtggtcgatt ttccggcccc gttcggcccg agaagcgcat
    80641 aaaaatcacc cgcttcgacc tgcaaatcga ttccacgaag cgcctgaacg ccgcctggat
    80701 aggttttttt aagctgttga agttccagtg caatggtcat aaatttttac ttaccttacg
    80761 ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta gccaaacgta
    80821 attatttggt tacaggtcgt taacctccat gaaagacata gatacactca tcagcaacaa
    80881 tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga aactggcaca
    80941 agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc ctgcagaacg
    81001 tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta acctggtcat
    81061 tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac tcgaagttga
    81121 acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag ttgaaaaccc
    81181 ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt tcaaacatag
    81241 ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg aactgaacgt
    81301 catggaacag gtgtataacc tgggccactc caccattatg caatcagcgt ggaaacgcgg
    81361 gcagaaagtt accattcacg gctgggccta cggcattcac gacggcttgc tgcgtgatct
    81421 ggatgtcaca gcgactaacc gcgaaaccct tgagcaacgt taccgtcacg ggatctccaa
    81481 cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca acacatccgg
    81541 caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag atgacggtaa
    81601 cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga gaaaccgata
    81661 aattctaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt acaaatcgcc
    81721 aacgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt attccccgag
    81781 tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc cagatctttg
    81841 aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc ggtcataaaa
    81901 tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat aaatgagcca
    81961 cgcagcagac caaccagcac catatcgctg ccactgtctt tgtaacgctc agtaatctga
    82021 cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac ttctacagta
    82081 tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg caggtttcag
    82141 caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc gcggaatctg
    82201 ttaataaagc tcgaattgtg atgacgatca cacatgttaa aacctataat taattgctac
    82261 taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac cggctcgcgg
    82321 cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta tctactcatt
    82381 ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc tggccttgtg
    82441 atgctcggcg tcgcctggat gctgtggcgc agtaaacgcg ccgcgctttg gctgtacgca
    82501 gccctgctgc tcggcactat gatttggggc gtctgggaag ttggtttcga cttctgggcg
    82561 ctgactccgc gcagcgacat tctggtcttc ttcggcatct ggctgatcct gccgtttgtc
    82621 tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt ggcactgctg
    82681 attagcggtg gtatcctggc ctgggccgga tttaacgatc cgcaggaaat cagcggcacc
    82741 ttaagcgccg ataccacgcc agcagaagcc atctcccccg ttgccgatca ggactggcct
    82801 gcctatggtc gcaatcagga aggtcaacgc ttttcacctc tgaaacaaat taacgctgat
    82861 aacgtccaca agctgaaaga agcctgggtg ttccgtactg gcgatgtgaa gcagccgaac
    82921 gatccaggtg aaatcaccaa tgaagtgacg ccaattaaag tgggcgacac cctttacctg
    82981 tgtaccgctc accagcgcct gtttgcgctc gatgccgcca gcggcaaaga gaaatggcat
    83041 tacgatcctg agctgaaaac caacgagtcc ttccagcatg taacctgccg tggcgtctct
    83101 tatcatgaag ccaaagcaga aactgcttcg ccggaagtga tggcggattg cccgcgtcgt
    83161 atcattcttc cggtcaatga tggtcgtctg attgcaatta acgctgaaaa cggcaagctg
    83221 tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc ggacaccaaa
    83281 ccgggtctgt atgaaccgac ttcgccgccg attatcaccg ataaaaccat cgtgatggcc
    83341 ggttcagtca ccgataactt ctcaacccgt gaaacatctg gcgtgatccg tggttttgat
    83401 gtcaacaccg gggagctgct gtgggctttt gatcctggtg cgaaagatcc taacgcaatc
    83461 ccgtctgacg aacacacctt tacctttaac tcgccaaact cgtgggcacc agcggcctat
    83521 gacgcgaagc tggatctggt gtatctgccg atgggcgtga ccacgccgga tatctggggc
    83581 ggtaaccgca caccggaaca ggaacgttat gccagctcga ttctggcgct gaatgccaca
    83641 accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga tatggatctt
    83701 ccggcacagc cgacgctggc ggatatcacc gttaatggtc agaaagtgcc agttatttat
    83761 gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gtaatggcga actggtggtt
    83821 ccggcaccgg aaaaaccggt tccccaaggt gctgccaaag gcgattacgt aaccccaact
    83881 caaccgtttt ctgaactgag cttccgtccg acgaaaaatt tgagcggtgc ggatatgtgg
    83941 ggagccacca tgtttgacca gctggtgtgc cgcgtgatgt tccaccagat gcgctatgaa
    84001 ggcattttca ccccgccatc tgaacagggt acgctggtct tccctggtaa cctggggatg
    84061 ttcgaatggg gcgggatttc cgttgatccg aatcgtgaag tggcaattgc caacccaatg
    84121 gcactgccgt ttgtttcgaa actgatccca cgcggtcctg gcaacccgat ggagcagccg
    84181 aaagatgcca aaggcacggg tacggaatcc ggcattcagc cgcagtacgg tgtaccgtat
    84241 ggcgtcacgc tcaatccgtt cctctcaccg tttggtctgc cgtgtaaaca gccagcatgg
    84301 ggttatatct cggcgctgga tctgaaaacc aatgaagtgg tgtggaagaa acgtattggt
    84361 acgccgcagg acagtatgcc attcccgatg ccggtacctg tgccgttcaa tatgggtatg
    84421 ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc cgctacggca
    84481 gataactacc tgcgcgctta taacatgagc aacggtgaaa aactgtggca aggtcgttta
    84541 ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga atggtaagca gtatgtggtg
    84601 atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat tgtggcttat
    84661 gcgttgccgg atgatgtgaa gtaaggcttg ctcagattgc tgacaacgtg cgatttgttc
    84721 atgccggatg cggcgtgaac gccttatccg gcctacaaaa catttgcaaa ttcaatacat
    84781 tgcgcgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtttcat
    84841 gcccggagag atccgggcat atttccgaat acggtctttt tataccgtaa accctaacat
    84901 catccccgta tcttcatgct ccagcaaatg gcagtgcgcc atataagcat gttctttcgg
    84961 tgcatcgtga ttaaacttca ccagcacttc gctgacatta ccttctacct taacggtatc
    85021 tttccagcct gcgcgatgcg tcgctggcgg tttgccattt tctgacaaga tacggaactg
    85081 cgtaccgtgg atatggaacg gatgcagcat catgtcgccc acgccagaga taacccaacg
    85141 ttcgtattgc cctttcgccg ccgcaaacat cggcttgttc atatcaaacg cctgaccgtt
    85201 gattttattg gcatggtgga aatcgaactt cccgccgtgg ttcatatgat tcatattgcc
    85261 gtgccccata tggcccatca tctggctgtg atccatcccg gccatcgcct gatcgccata
    85321 tttctccatc agcatctgca tccccatcat atcgagcatt gggtccatag aaagttgcag
    85381 cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc aggctactta atgtgtctgg
    85441 caaagcacca gaggcactaa tggcaatcgg ctgaatccgc attaccggat gcggcttgtc
    85501 aaacggcgca atcgccatcc ccatctggct gaccggcagc gtcaccaggt caaagggttt
    85561 gttgtcgtta acctccacca gcacttcaaa acgctcgccc atcagcactg gcagctcgtt
    85621 caccttcacc ggttcaggta gcagaccgcc gtcgctggca atcacataaa gcgggcgatt
    85681 gtcgctggtg gcgaaattga gcgagcgggc attacagcca ttgagcaaac gcaggcgcag
    85741 ccagccacgc ggggcagcgt gttgtgggta gattacaccg ttggtcagca acgtatcgcc
    85801 aaaccaaccc acagcggcgg tcatcacatc cagttgataa tcaatctgcc cgtcagcgtt
    85861 aaatttttta tcctgaacaa taaccggaac atcatcaata ccccactgtt ttggcagcat
    85921 taatttcagg atctcgtcat cttcaatcac caccaaacca gccagcccca tcgccacctg
    85981 acgtccggtc ttgccatgtt gatgtggatg gaaccagcag gtagcggcag gttgatcaac
    86041 gttcaacgtc accgagcgct tgccacctgg cggaataatt ccctgcgggc cgccgtccac
    86101 ttcacccggt acttccagcc cgtgccagtg caacgtcgtc tcttccgtca gttggttgta
    86161 gatatcaacc gttaccgctt tgccgcgctg taatttcacc gccggcccca gcagattgcc
    86221 gttatagccc caggtagttg cggttttccc gccaaaggtg gactgacctg cgccaatagt
    86281 taactgaatg cgattacggg catcggtcgt gagcaaatca gggatcggta acgttgggcg
    86341 ttccgccgca aatactgcgc ggctccacag cggcaaagcc gaagccacgc ccagcgcgac
    86401 ggaatatttt aagaaatcac gacgttgcat agttatttcc ttattcttaa gcaggcgaaa
    86461 caaaatcaaa cgttgagcat agtccttccc cttacgggaa ggtcaagccg cagacacaat
    86521 ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat cttctttaag
    86581 cccctggtag aagcaatgaa gacgtttttc agaacagtgt tattcggcag cctgatggcc
    86641 gtctgcgcaa acagttacgc gctcagcgag tctgaagccg aagatatggc cgatttaacg
    86701 gcagtttttg tttttctgaa gaacgattgt ggttaccaga acttacctaa cgggcaaatt
    86761 cgtcgcgcac tggtcttttt tgcccagcaa aaccagtggg atctcagtaa ttacgacacc
    86821 ttcgacatga aagccctcgg tgaagacagc taccgcgatc tcagcggcat tggcattccc
    86881 gtcgctaaaa agtgcaaagc cctggcccgc gattccttaa gcctgcttgc ctacgtcaaa
    86941 taatccctga tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa tgttagctat
    87001 tatgttgcgc ccttttttta cgggtgttaa caaaggaggt atcaacccat ggccgaaaaa
    87061 aaacagtggc atgaaacgct acacgaccag tttgggcagt actttgcggt agataacgtt
    87121 ctgtatcatg aaaagaccga tcaccaggat ctgatcattt ttgagaacgc cgcatttggt
    87181 cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc gcgacgagtt tatctatcat
    87241 gagatgatga cccatgttcc gctactggcc cacggtcacg cgaaacatgt gctgattatc
    87301 ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt tgagtcaatc
    87361 acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc gtcagtatct acccaaccat
    87421 aacgccggta gctacgacga tccgcgcttt aaactggtga tcgacgatgg cgtcaatttc
    87481 gttaatcaaa ccagccagac ctttgatgtc attatttccg actgcaccga tcctatcggt
    87541 cccggcgaaa gtctgttcac ctcagcattt tatgaaggct gcaaacgttg cctgaatcct
    87601 ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga ggccatcgac
    87661 agccatcgca aactcagcca ttacttcagc gatgttggct tttatcaggc ggcgatcccg
    87721 acctattacg gcggcatcat gacctttgca tgggcgacag ataacgacgc cttacgccat
    87781 ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg gcctgaaatg ccgttattac
    87841 aatccggcag tccatacggc agcttttgcc ttaccccagt atctgcaaga tgcactggct
    87901 tcacagccgt cctaaggaga agataagaaa ttgaaaaaac tgaaactgca tggctttaat
    87961 aatctgacca aaagtctgag tttttgtatt tacgatatct gctacgccaa aactaccgaa
    88021 gagcgcgacg gttatattgc ttatatcgat gaactctata atgccaaccg tctgaccgaa
    88081 atcctgtcag aaacctgttc cattatcggg gccaatattc ttaacatcgc ccgccaggat
    88141 tacgagccac aaggtgccag cgtcactatt ctggtgagtg aagaaccggt cgacccgaaa
    88201 cttatcgaca aaacagaaca ccccggccca ctgccagaaa cggtcgttgc ccatctcgat
    88261 aaaagtcata tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg tttatgtacc
    88321 ttccgcgccg atattgaagt ctctacctgc ggcgtgattt ctccgctgaa ggcgctgaat
    88381 tacctgatcc accagcttga gtccgatatt gtgactattg attatcgcgt gcgcggtttt
    88441 acccgcgaca ttaacggtat gaagcacttt atcgaccatg agattaattc gattcagaac
    88501 tttatgtctg acgatatgaa ggcgctgtat gacatggtgg atgtaaacgt ctatcaggaa
    88561 aatatcttcc ataccaagat gttgcttaaa gagttcgacc ttaagcacta catgttccac
    88621 accaaaccgg aagatttaac cgacagcgag cgccaggaaa ttaccactgc gctgtggaaa
    88681 gaaatgcgcg agatttatta cgggcgcaat atgcccgctg tttaacgtct caggcggagc
    88741 acggaggctc cgcctgattt acttacttct gctgcacgaa attacggtaa gccgccacca
    88801 cctgaagaaa atcctcaacg ccgcacagcg acaagctctc ttcatcgtag tagttcatcc
    88861 cctcttccat ttcatcgcca gcgaattcca gttgattggc gcgaaccatc acctcttcac
    88921 catccatcca tagcgtgtat tcatgccctg cccgttgcca ggaacgttcg ctacctttca
    88981 atgcgtgcgc cgcttgttct acttcatcaa gcaaggccag gttttctttc acctcttcat
    89041 taaaccagtg cccgaccact tcatgcccca tggacatgcg cacctttacc actccggtaa
    89101 tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc tcctggcaat gcgccgaaat
    89161 cgcattgcgc cattgatgta attatcgcag ccttaccgga gaaaaacagc ggaacggcga
    89221 tggtttagaa ataaaaaatg cccgggaatc tcattcaccg ggcattgtgt cgtttatgcg
    89281 cagcgcgtgc gcttacttat taaaccgcag tctggaaaat caccccgtcc gctttttcgg
    89341 tgtactgaga aagctggtcg aagttcagat aacggtaagt atcaacggct gttttatcta
    89401 cctgcgccac gtaggtctgg tactcttccg gcgtcggcag tttgccaatc agcgccgcaa
    89461 cagcagccag ttccgcagaa gccaggaaga cattcgcgcc agtacccaaa cggttcggga
    89521 agttacgggt agaggtggaa accaccgtcg caccgtctgc tacacgcgcc tggttaccca
    89581 tacacaggga acagccaggg atctcgatac gagcaccact cttaccgaag acgctgtagt
    89641 agccttcttc ggtcagctgc gcagcgtcca tacgcgttgg cggtgccacc cacaggcggg
    89701 tcggcaactg acctttgtgc gcatccagca gtttaccagc agcacggaag tgaccgatgt
    89761 tagtcatgca agaaccgata aatacttcgt cgatcttctc gccctgtacc gcagacagcg
    89821 gacgtgcgtc gtccgggtca ttcggtgcac agaggattgg ctctttgata tccgccagat
    89881 cgatgtcgat cactgccgcg tattccgcat ctgcatcggc ttccagcagc tcaggattcg
    89941 ccagccattt ttccatgccc tgaatacgac gctccagggt acgacgatcg ccgtaacctt
    90001 ccgcgatcat ccacttcagc aggacgatgt tagagttcag gtattcaatg atcggttctt
    90061 tgttcagctt gatggtacaa ccggcagcag aacgctcggc agacgcatcg gtcagctcaa
    90121 atgcctgctc aactttcaga tccggcagac cttcaatttc cagaatgcgg ccagagaaga
    90181 tgtttttctt acctttcttc tcaacggtca gcagaccttg tttgattgca tacagcggga
    90241 tcgcgtgtac cagatcgcgc agggtgatac ccggctgcat tttgcctttg aagcgcacca
    90301 gaacggattc tggcatatcc agcggcatta cgcctgttgc ggctgcaaat gccaccagac
    90361 cagaacccgc cgggaaagag ataccgatcg ggaaacgggt atgggagtca ccaccggtac
    90421 cgacagtatc cggcagcagc atacggttca gccaggagtg gattacgccg tcacccggac
    90481 gcagcgacac accgccacgg ttcataatga agtccggcag cgtgtggtgc gtgttaacgt
    90541 caactggttt cggatacgcc gcggtgtggc agaaagactg catcaccagg tcagccgaga
    90601 agcccaggca cgccaggtct ttcagttcat cacgggtcat cgggccggtg gtgtcctggg
    90661 aacctacaga agtcattttc ggttcacagt acgcgcccgg acgaatgcct ttcacgccac
    90721 aggcacggcc taccattttc tgcgccagcg agaagccgcg atcgctctca gcgacatctt
    90781 tcgcctgacg gaacacatcg ctgtgcggca ggccaagtgc ttcacgcgct ttggtggtca
    90841 ggccacgccc aataatcagc ggaatacggc cgccagcacg cacttcatca atcagcacgt
    90901 cggttttcag ttcgaaggtc gccagcagtt cgccggtttc gtggttacgc acttcacctt
    90961 tgtacgggta aacgtcaatt acgtcgccca tgttcaggtt agagacgtcg acttcgattg
    91021 gcagcgcacc cgcgtcttcc atcgtgttaa agaagatggg tgcaatttta ccgccgaggc
    91081 acaaaccacc gccgcgtttg ttcggcacat gtggaatatc atcgcccata aaccacagaa
    91141 cggagttagt ggcggattta cgcgatgacc ccgtacccac aacgtcaccg acgtacgcca
    91201 gtgggaaacc tttctgttgc agagcttcga tttgcttaat cggaccaaca acgcctggct
    91261 gatctggctc aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc agtgggatat
    91321 ccgggcgtga ccacgcatcc ggtgccggag agaggtcatc ggtgttggtt tcgcctgtga
    91381 ctttgaagac ggtaacggtc agtttttcag ccagcgccgg gcgattcagg aaccattcgg
    91441 catccgccca ggactgcatt acctgcttcg catattcgtt gcctgctttc gctttctctt
    91501 ctacgtcata gaagttatcg aacatcagca gcgtgtgaga aagtgctttg gcagcgatcg
    91561 gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa ccaccctgca
    91621 tggtgcccag cagttcgatg gctttttccg gagtcagcag aggggatttg gcttcgcctt
    91681 tagcgatagc agccaggaag cctgctttta cataggcggc ttcatcgacg cctgggggaa
    91741 cacggttggt taacagatct aacaggaatt cttcttcgcc cgcgggcggg tttttcagca
    91801 gctctacaag cgcggccatt tggtttgcat ccaggggttt gggcgcaatc ccctcagcgg
    91861 cacgctcagc tacgtgctta cggtattctt ctagcacgac ggttctcctc gctctcattg
    91921 tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg tagggtaaat
    91981 gcccagtacc gcaacgggca gaatagcagg attttgacga tgtgttaatc tgtttacaaa
    92041 aaagcaacat aaaaaagtgg ctgaatcgtt aaggatggtc tagagattgt ttccctccgt
    92101 aaaaacttcg ttaattcgca tggtgataat cacctttctc aacgaatccc aagattagtc
    92161 aaaatttaaa ctaccgcctc tttatactcg gattcacagc acctgcgggt ggcagttcgc
    92221 cgaccattgc gatttccttg agatccgaat tatgaaaatg actttgccgt ttaaacccca
    92281 tgtgctggcg ctaatttgca gtgccgggct ttgtgccgcc tctaccgggc tatatataaa
    92341 aagccgcaca gtggaagcgc ctgtggaacc gcaatcgaca caactggctg cgcctgacat
    92401 ctccgcagtt acgcttcctg caacggtttc cgtgcctcct gtaacacctg ccgtcgtcaa
    92461 atccgcattc agcactgcgc aaatagatca atgggttgca cctgtcgcgc tataccccga
    92521 ctctctgctt tcacaagtgt taatggcatc aacctatccg gcaaacgttg ctcaagcagt
    92581 gcaatggtcg cacgataatc cacttaaaca aggcgatgct gctattcagg cggtatctga
    92641 ccagccctgg gacgccagcg tcaaatcact ggtggccttt ccacagttga tggcattgat
    92701 gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc agccgcagga
    92761 ggtgatggac tcggtacagc gattgcggca actggcgcaa caaaccggct cgctgaagtc
    92821 atcaactgaa cagaaagtta ttaccacaac gaagaaagct gtaccggtaa aacagacagt
    92881 cacggctccc gtcataccat ccaataccgt ttcaactgcc aaccctgtca ttacagagcc
    92941 tgcaacaacc gtcatttcca ttgaacccac caatcctgat gtggtctata ttcccaacta
    93001 caacccaacc gtggtttacg ggaactgggc caatactgcg tatccgccgg tttatctgcc
    93061 gccgccagcc ggagaaccgt ttgttgacag ctttgtgcgc ggattcggct atagcatggg
    93121 cgttgctacc acgtacgcgc tattcagcag catcgactgg gatgacgacg atcatgacca
    93181 tcatcatcat gacgatgatg attatcacca tcacgatggc ggtcatcgtg atggtaatgg
    93241 ctggcaacac aatggcgaca acatcaatat cgacgtcaac aatttcaacc gtatcaccgg
    93301 tgaacatctt actgataaga atatggcatg gcggcacaat ccaaactacc gtaatggtgt
    93361 gccctatcat gatcaggata tggcaaagcg gtttcatcaa accgatgtca acggcggaat
    93421 gagcgccacg cagctacctg ctccaacacg cgacagccag cgtcaggcgg cagcaagtca
    93481 gtttcagcaa cgaacacaca gcgcaccagt cattacacga gatacccaac gtcaggcagc
    93541 ggcacagcgg tttaatgaag ctgaaaacta tgggagctat gacgacttcc gcgacttcag
    93601 ccgtcgccaa ccactgaccc agcaacaaaa ggacgccgct cgtcagcgtt atcagtcggc
    93661 ctctcctgag cagcgccagg cagtccgcga gaaaatgcag actaacccgc agatccagca
    93721 gcgaagagag gcagcgcgtg agcgcattca gtctgcaact cccgaacagc gccaggtgtt
    93781 taaggaaaaa gtacagcagc gcccactgaa tcaacagcaa cgtgataacg cccgccagcg
    93841 tgttcaatca gcatcacctg agcaacgtca ggtttttcgg gagaaagttc aggagagccg
    93901 cccgcaacgt ctaaacgaca gtgaccatac tgtcaggctg aataacgagc aacggtcagc
    93961 agtacgcgaa cgtctctctg agcgcggagc aaggcgactg gaaaggtaaa ttacagacgt
    94021 aaaaaaagcg gcgtggttag ccgctttttt aattgccaga tgttccggca aacgaaaaat
    94081 tacttcttct tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc
    94141 gccaggccca cagactcgtg cagagtcggg tgcgcatgga tggtcagtgc gatgtcttca
    94201 gcgtcacaac ccatttcgat tgccaggccg atttcaccca gcaactcgcc gccgttagtg
    94261 ccgacaatcg caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata
    94321 ccgtctgcgc agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt
    94381 tcgtagctga tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct
    94441 ggttcggtat aggcgatgga cgggataact ttcggatcga agtaatgttt tttaccggcg
    94501 ataacttcag cggcaacgtg accttcgtga acacctttgt gtgccagcat tggctgaccg
    94561 acgatatcac cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg
    94621 atgaaaccac ggtcgtcaac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc
    94681 ggcacacgac caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt
    94741 ttgccttcca tcgtcacata aatgccgtct tctttcgctt caacggcggt aactttggtt
    94801 tccagcatca ggttgaattt cttgctgata cgcttggtaa agactttaac gatgtcttta
    94861 tcagctgccg ggatgacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg
    94921 tggtatacgg tgcccatttc cagaccgatg ataccgccac ccattaccag caggcgttct
    94981 ggtacttctt tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga
    95041 ataaacggca gttgaatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc
    95101 acggttttac cgttctcacc ttcaacttcc agggtgttag ccccggtaaa tttacccaga
    95161 ccgttgacca ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga
    95221 ttgatcactt tctctttcca ggtacgaatc ttgtcgatat cggttttcgg ttcgccaaag
    95281 acgataccgt gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt
    95341 gctttagaag ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtagcgttct
    95401 acgattacgg tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg
    95461 cctgccccaa gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat
    95521 ttatctccgg cggtcatacc cgtcgtcttt caggccgcag cggcgttagc ttcacgccct
    95581 caccccagtc acttacttat gtaagctcct ggggattccg gcgcttgcta ccttgctgca
    95641 cccagaaatc catagggtat gtgaagtaat accctaacca ccaccgggtc gttctatccg
    95701 tcggtatttt acaaaattgt taacaatttt taaacaacaa acggcaaccg atttgtctat
    95761 tcgctaataa cctcaataca ttcatgagat taccagaaaa aagccggccg ttgggccggc
    95821 tcttttactt acatcaccag acggcgaatg tcagacagcg tgttgttaat gatggtaatg
    95881 aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga aatcggcagc
    95941 atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga cttggaaacg
    96001 ccgaggatag ccacttccgg cgcgttcaca atcggcgcga agtgggtagt acccaggccg
    96061 ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca
    96121 cgcgctttct tagaaatagt catcagctcg cgagacagct cgatgatgcc tttcttgttg
    96181 acgtctttga ataccggaac aaccagaccg ttcggggtat ccaccgccac accgatgttg
    96241 atgtatttct tcagggtcag acgctgaccg tcttccgaca gcgaactgtt gaagcgaggc
    96301 atctgctcaa gagctgcagc aacggctttc atgatgaaga caaccggggt gatcttcaca
    96361 tccagcttac gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg
    96421 atatcggttt tgtcgaagtg ggtaacatgc gggatcatca cccagttacg gctcaggttc
    96481 gcaccagaga ttttctggat acggcccagt tccacttctt cgatttcacc aaacttgctg
    96541 aagtccacct tcggccacgg cagcatgccc gggataccgc cgccagtcgc cgccggagcc
    96601 gcttctgcac gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg caggatacga
    96661 cctttacggc cagtgccctt cactttcgcc aggttaacgc caaattcgcg tgccagacgg
    96721 cggatcagcg gagtcgcgtg aacgtaagcg tcgttttcag caaattcaga tttgccttcg
    96781 gctttcgcag ccggagctgc tgctggagct tcagcttttg ctgccggagc cggcgctgcc
    96841 gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc
    96901 agcgagccag ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca
    96961 aacggagccg gaacttccat agacgcttta tcgccttcta cggtgatcag tgactgttca
    97021 gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcaccgccg
    97081 atatccggaa cgttaacgtc tttcacgcca gccgctggtg cagaggccgc tgccggagcc
    97141 gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata
    97201 atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtgcca
    97261 gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtgat cagcgactgt
    97321 tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg
    97381 ccgatatccg gaacgttaac gtcttttgcc gctgcagccg ctggagctgc tgccggagcg
    97441 gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg
    97501 aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta
    97561 acgatacccg cctgcggaga cggaacttcc atagaggctt tgtcgccttc tacggtgatc
    97621 agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact
    97681 tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg
    97741 ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca
    97801 ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc acgacataag
    97861 aagcatcaac ctcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat
    97921 cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacttgc tcagcgaaca
    97981 gtttcatata gtcggtagat gccactgccg gagcgtcgtt catcacctga gcgatatacg
    98041 gaacgcgcgg agtttccagc gggtgcagca tgttccagcg ttcacaatcc tgaccatcac
    98101 gcgccagttc ggtgaaggaa gtcacgctat aaacgtcaga acctacgccg taatctttcg
    98161 ccaggatctc tgctgcttca cggacgtgac gcaggataga accggagccc agcagctgaa
    98221 ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct
    98281 cagcaccttc cggcattgct ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt
    98341 aaacgttctc ttgtttctca ccgtacatac gctccagacc gtcatgcata atgacagcaa
    98401 cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg gatagtcagc gactgaatgt
    98461 ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag ggtggtacga ccggaagtac
    98521 cgccgatcag gaagccacgc gcttgctggt caccagccgc ccagcacaga tcgccgatac
    98581 gctggaagcc gaacatcgag taatagatgt agaacggaat catcggcaga ttgttggtgc
    98641 tgtaagaggt cgccgctgcc agccaggaac acccagcgcc cagctcgttg atcccttcct
    98701 gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg
    98761 tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga
    98821 aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca
    98881 gcattacgtt cagggcacga acgaaagcga tagtggtaga gatctctttg ctctgctctt
    98941 ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct
    99001 gacggcttgg cagataaccg tgcagtttct gacgctgagc gtgcagatag gtatgctctt
    99061 cagaaccttc cgggaaggtg atgtacggca atttttcgat atcggcatca gacaccggca
    99121 cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg
    99181 cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggta tgagcaagga
    99241 ttaccgtcgc tttgcctttg gtttcctgcg ctttcttaaa tgcagcgtag attttcttcg
    99301 gatcgtgacc accacggttc agtgcccaga tctgttcgtc agtccagtct gcaaccagtg
    99361 ctgcggtttc aggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt
    99421 tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg
    99481 tatctttacg cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc
    99541 cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc
    99601 cgtcaagacg ctgcaggtta cagttgataa cgaaaaccag gttatccagt ttttcacggg
    99661 tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg
    99721 cgtaaacggt ttgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt
    99781 tagcctggta aatagcacca atcggcccca gacccataga tacggtcggg aactgccaga
    99841 attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac
    99901 ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacga gcgtaaacgc
    99961 ccggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac
   100021 ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatgt
   100081 ggccgcccag ttcgaggtct tttttcgacg cacgcagaac cgtcatgatg gcgttccagc
   100141 ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt
   100201 caacggggat ggtgttgatg tagttgctga taccggtgcc tgcggctacg ttgacaccgc
   100261 ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt
   100321 cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat
   100381 ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga
   100441 acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg tttagttgcc
   100501 gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac
   100561 taattctttc gttgctccag acgacgtaga gaacgctcac ggcggctctc ttcacggctt
   100621 ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct
   100681 tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc
   100741 agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc
   100801 ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc ggtgacggca
   100861 atctgatact ggagtacggc atttgattcc gcgtccagat cgccagactg ctgcgccagc
   100921 tctatggcat ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg
   100981 gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat
   101041 tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc
   101101 tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga
   101161 atcgcctcac gcaaggaggg acgggagacg tcgaactgtt ttgccagttc gcgttccggt
   101221 gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca
   101281 atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag
   101341 ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag
   101401 tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca
   101461 acttgacata tataacgttt caaagttgta actatgcaca aatgttaaat ttacccatca
   101521 aggggtgaga ttaacagaat tgaaacgaaa aaatatgtcc tctgaaccga ttcaccaagc
   101581 aacaaaacgc ataacaattc aattatgtga attaatatgt aaaatgaatg ttcagggaag
   101641 gagagaaagg tagtatttac ctatgttttc tttttacatt gcttattatc actgcaatcg
   101701 ccaccgtaac cttctcgtag cgaaaatcat cacttaacca gaacgtgatg attttcaggt
   101761 gacagattct gtttagaatt cattctgaat ctttgaacaa agacaatcct tgcgaacgcc
   101821 gccattcttt gacgattttc aggatgtttt caggcttatc atctcccttc tttgcaggat
   101881 agtagataag atcgctcatc aacggatgtt gtgtaattct ggtaagatgt tcttctagat
   101941 catcccaata tttgtcaagc tcttcccctt ttaattgagc gttatccccg gttggatttg
   102001 taaactcgcc aagcaactca atgaattccc gctctgtgta atcttctact ttatctttaa
   102061 aattatacat ttaactatct cctgtaataa atttcatcat gcaaatgagg tgtgccctcc
   102121 ttaaattatc aatatcgtaa atttcgccac tatattcaat cgcaacacaa tgatacatgt
   102181 ggaatttttt aacgatttct ttagggccat aatgccaacc ttcaagaggt gcttttgcga
   102241 ttaatccttg ttttatacgt gtttgattac cacttgaaaa ctgcgctatc aactccggat
   102301 actgactcac ctccagccaa agggtatcgg tgaaagcggg aaattgataa aataacctaa
   102361 tgagatttca gtattttacc tgttttttaa gagcacccac attattattt tatgttatta
   102421 acatcatcgc attagccaga cgcgatgatg ttccatatat agttttatta tctactttgt
   102481 ttagagtctt taaacaaggg tagcccctgt gaacgccgcc attctttgac aattttcagg
   102541 atgttttcag gcttatcatc tccttgactt ttagggtaga aaattagatc gctccctgac
   102601 ggatgctgtg taatttcaat aaaatgatct actaatgaat cccagtacat ttctagctcc
   102661 ttacctttta agggctttcc atctttcata gttgatctca atccgcctaa aaaattaaga
   102721 aattcctcct cggtataatc ttcgagcttt tctttaaaca tatttatctc ctgtagtgaa
   102781 tctcatcgtg tagtctgggg gtaacaattc gtaaattatc aatatcgtat acgctgccgc
   102841 catattcaat tgcaacacga tgatgtatct ggaatttttt aacgatttct ttagggccat
   102901 aacgccaacc ttcaagaggt gcttttgcgg ttaatccttg tttta
//
LOCUS       DAZGTJ010000006       100416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000006 DAZGTJ010000000
VERSION     DAZGTJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.006"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(119..1030)
                     /gene="gsiD"
                     /locus_tag="ACHK8E_000562"
     CDS             complement(119..1030)
                     /gene="gsiD"
                     /locus_tag="ACHK8E_000562"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP0342880.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1033..1953)
                     /gene="gsiC"
                     /locus_tag="ACHK8E_000563"
     CDS             complement(1033..1953)
                     /gene="gsiC"
                     /locus_tag="ACHK8E_000563"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP0342881.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1971..3509)
                     /gene="gsiB"
                     /locus_tag="ACHK8E_000564"
     CDS             complement(1971..3509)
                     /gene="gsiB"
                     /locus_tag="ACHK8E_000564"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP0342882.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3529..5400)
                     /gene="gsiA"
                     /locus_tag="ACHK8E_000565"
     CDS             complement(3529..5400)
                     /gene="gsiA"
                     /locus_tag="ACHK8E_000565"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP0342883.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5387..6352)
                     /gene="iaaA"
                     /locus_tag="ACHK8E_000566"
     CDS             complement(5387..6352)
                     /gene="iaaA"
                     /locus_tag="ACHK8E_000566"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0342884.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6556..7791
                     /gene="moeA"
                     /locus_tag="ACHK8E_000567"
     CDS             6556..7791
                     /gene="moeA"
                     /locus_tag="ACHK8E_000567"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP0342885.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7791..8540
                     /gene="moeB"
                     /locus_tag="ACHK8E_000568"
     CDS             7791..8540
                     /gene="moeB"
                     /locus_tag="ACHK8E_000568"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP0342886.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(8616..9278)
                     /gene="fsa"
                     /locus_tag="ACHK8E_000569"
     CDS             complement(8616..9278)
                     /gene="fsa"
                     /locus_tag="ACHK8E_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0342887.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9409..10308
                     /gene="ybiY"
                     /locus_tag="ACHK8E_000570"
     CDS             9409..10308
                     /gene="ybiY"
                     /locus_tag="ACHK8E_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP0342888.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10314..12746
                     /gene="ybiW"
                     /locus_tag="ACHK8E_000571"
     CDS             10314..12746
                     /gene="ybiW"
                     /locus_tag="ACHK8E_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP0342889.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12892..13707
                     /gene="ybiV"
                     /locus_tag="ACHK8E_000572"
     CDS             12892..13707
                     /gene="ybiV"
                     /locus_tag="ACHK8E_000572"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP0342890.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            13859..15118
                     /locus_tag="ACHK8E_000573"
     CDS             13859..15118
                     /locus_tag="ACHK8E_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP0342891.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(15359..16951)
                     /gene="ybiT"
                     /locus_tag="ACHK8E_000574"
     CDS             complement(15359..16951)
                     /gene="ybiT"
                     /locus_tag="ACHK8E_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP0342892.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17170..18090
                     /gene="ldtB"
                     /locus_tag="ACHK8E_000575"
     CDS             17170..18090
                     /gene="ldtB"
                     /locus_tag="ACHK8E_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0342893.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18148..19266)
                     /gene="ybiR"
                     /locus_tag="ACHK8E_000576"
     CDS             complement(18148..19266)
                     /gene="ybiR"
                     /locus_tag="ACHK8E_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP0342894.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19263..19730)
                     /gene="mntR"
                     /locus_tag="ACHK8E_000577"
     CDS             complement(19263..19730)
                     /gene="mntR"
                     /locus_tag="ACHK8E_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP0342895.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVYMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            19916..20044
                     /gene="mntS"
                     /locus_tag="ACHK8E_000578"
     CDS             19916..20044
                     /gene="mntS"
                     /locus_tag="ACHK8E_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP0342896.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20316..21899
                     /gene="opgE"
                     /locus_tag="ACHK8E_000579"
     CDS             20316..21899
                     /gene="opgE"
                     /locus_tag="ACHK8E_000579"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0342897.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(21948..22463)
                     /gene="ompX"
                     /locus_tag="ACHK8E_000580"
     CDS             complement(21948..22463)
                     /gene="ompX"
                     /locus_tag="ACHK8E_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP0342898.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22516..22581)
                     /locus_tag="ACHK8E_000581"
     CDS             complement(22516..22581)
                     /locus_tag="ACHK8E_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342899.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22816..23703
                     /gene="rhtA"
                     /locus_tag="ACHK8E_000582"
     CDS             22816..23703
                     /gene="rhtA"
                     /locus_tag="ACHK8E_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP0342900.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24003..24506
                     /gene="dps"
                     /locus_tag="ACHK8E_000583"
                     /gene_synonym="pexB"
     CDS             24003..24506
                     /gene="dps"
                     /locus_tag="ACHK8E_000583"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP0342901.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            24910..25656
                     /gene="glnH"
                     /locus_tag="ACHK8E_000584"
     CDS             24910..25656
                     /gene="glnH"
                     /locus_tag="ACHK8E_000584"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP0342902.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            25795..26454
                     /gene="glnP"
                     /locus_tag="ACHK8E_000585"
     CDS             25795..26454
                     /gene="glnP"
                     /locus_tag="ACHK8E_000585"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP0342903.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26451..27173
                     /gene="glnQ"
                     /locus_tag="ACHK8E_000586"
     CDS             26451..27173
                     /gene="glnQ"
                     /locus_tag="ACHK8E_000586"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP0342904.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            27290..29515
                     /gene="ybiO"
                     /locus_tag="ACHK8E_000587"
     CDS             27290..29515
                     /gene="ybiO"
                     /locus_tag="ACHK8E_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP0342905.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(29512..30438)
                     /gene="rlmF"
                     /locus_tag="ACHK8E_000588"
     CDS             complement(29512..30438)
                     /gene="rlmF"
                     /locus_tag="ACHK8E_000588"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP0342906.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            30714..30974
                     /gene="mcbA"
                     /locus_tag="ACHK8E_000589"
     CDS             30714..30974
                     /gene="mcbA"
                     /locus_tag="ACHK8E_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP0342907.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            31239..33521
                     /gene="fiu"
                     /locus_tag="ACHK8E_000590"
     CDS             31239..33521
                     /gene="fiu"
                     /locus_tag="ACHK8E_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP0342908.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            33563..34240
                     /gene="ybiX"
                     /locus_tag="ACHK8E_000591"
     CDS             33563..34240
                     /gene="ybiX"
                     /locus_tag="ACHK8E_000591"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP0342909.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            34314..34580
                     /gene="ybiI"
                     /locus_tag="ACHK8E_000592"
     CDS             34314..34580
                     /gene="ybiI"
                     /locus_tag="ACHK8E_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP0342910.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            34845..35105
                     /gene="ybiJ"
                     /locus_tag="ACHK8E_000593"
     CDS             34845..35105
                     /gene="ybiJ"
                     /locus_tag="ACHK8E_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP0342911.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35245..36330)
                     /gene="hcxB"
                     /locus_tag="ACHK8E_000594"
     CDS             complement(35245..36330)
                     /gene="hcxB"
                     /locus_tag="ACHK8E_000594"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP0342912.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(36471..37433)
                     /gene="ybiB"
                     /locus_tag="ACHK8E_000595"
     CDS             complement(36471..37433)
                     /gene="ybiB"
                     /locus_tag="ACHK8E_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP0342913.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(37461..39611)
                     /gene="dinG"
                     /locus_tag="ACHK8E_000596"
     CDS             complement(37461..39611)
                     /gene="dinG"
                     /locus_tag="ACHK8E_000596"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP0342914.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            39715..39915
                     /locus_tag="ACHK8E_000597"
                     /pseudo
     CDS             39715..39915
                     /locus_tag="ACHK8E_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(40150..41511)
                     /gene="rhlE"
                     /locus_tag="ACHK8E_000598"
     CDS             complement(40150..41511)
                     /gene="rhlE"
                     /locus_tag="ACHK8E_000598"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP0342915.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            41740..42411
                     /gene="cecR"
                     /locus_tag="ACHK8E_000599"
     CDS             41740..42411
                     /gene="cecR"
                     /locus_tag="ACHK8E_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP0342916.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            42414..43409
                     /gene="hlyD"
                     /locus_tag="ACHK8E_000600"
     CDS             42414..43409
                     /gene="hlyD"
                     /locus_tag="ACHK8E_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP0342917.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            43402..45138
                     /gene="ybhF"
                     /locus_tag="ACHK8E_000601"
     CDS             43402..45138
                     /gene="ybhF"
                     /locus_tag="ACHK8E_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0342918.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            45131..46264
                     /gene="ybhS"
                     /locus_tag="ACHK8E_000602"
     CDS             45131..46264
                     /gene="ybhS"
                     /locus_tag="ACHK8E_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0342919.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            46275..47381
                     /gene="ybhR"
                     /locus_tag="ACHK8E_000603"
     CDS             46275..47381
                     /gene="ybhR"
                     /locus_tag="ACHK8E_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0342920.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(47343..47753)
                     /gene="ybhQ"
                     /locus_tag="ACHK8E_000604"
     CDS             complement(47343..47753)
                     /gene="ybhQ"
                     /locus_tag="ACHK8E_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP0342921.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            47886..48647
                     /gene="ybhP"
                     /locus_tag="ACHK8E_000605"
     CDS             47886..48647
                     /gene="ybhP"
                     /locus_tag="ACHK8E_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0342922.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            48644..49885
                     /gene="clsB"
                     /locus_tag="ACHK8E_000606"
     CDS             48644..49885
                     /gene="clsB"
                     /locus_tag="ACHK8E_000606"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP0342923.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            49885..50841
                     /gene="ybhN"
                     /locus_tag="ACHK8E_000607"
     CDS             49885..50841
                     /gene="ybhN"
                     /locus_tag="ACHK8E_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP0342924.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(50877..51266)
                     /locus_tag="ACHK8E_000608"
     CDS             complement(50877..51266)
                     /locus_tag="ACHK8E_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0342925.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(51472..52176)
                     /gene="ybhL"
                     /locus_tag="ACHK8E_000609"
     CDS             complement(51472..52176)
                     /gene="ybhL"
                     /locus_tag="ACHK8E_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0342926.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(52313..52765)
                     /gene="moaE"
                     /locus_tag="ACHK8E_000610"
     CDS             complement(52313..52765)
                     /gene="moaE"
                     /locus_tag="ACHK8E_000610"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP0342927.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(52767..53012)
                     /gene="moaD"
                     /locus_tag="ACHK8E_000611"
     CDS             complement(52767..53012)
                     /gene="moaD"
                     /locus_tag="ACHK8E_000611"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP0342928.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(53005..53490)
                     /gene="moaC"
                     /locus_tag="ACHK8E_000612"
     CDS             complement(53005..53490)
                     /gene="moaC"
                     /locus_tag="ACHK8E_000612"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP0342929.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(53493..54005)
                     /gene="moaB"
                     /locus_tag="ACHK8E_000613"
     CDS             complement(53493..54005)
                     /gene="moaB"
                     /locus_tag="ACHK8E_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP0342930.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(54027..55016)
                     /gene="moaA"
                     /locus_tag="ACHK8E_000614"
     CDS             complement(54027..55016)
                     /gene="moaA"
                     /locus_tag="ACHK8E_000614"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP0342931.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(55004..55148)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            55413..56321
                     /gene="yvcK"
                     /locus_tag="ACHK8E_000615"
     CDS             55413..56321
                     /gene="yvcK"
                     /locus_tag="ACHK8E_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP0342932.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(56513..58534)
                     /gene="uvrB"
                     /locus_tag="ACHK8E_000616"
     CDS             complement(56513..58534)
                     /gene="uvrB"
                     /locus_tag="ACHK8E_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP0342933.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(59113..59790)
                     /gene="bioD"
                     /locus_tag="ACHK8E_000617"
     CDS             complement(59113..59790)
                     /gene="bioD"
                     /locus_tag="ACHK8E_000617"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0342934.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(59783..60538)
                     /gene="bioC"
                     /locus_tag="ACHK8E_000618"
     CDS             complement(59783..60538)
                     /gene="bioC"
                     /locus_tag="ACHK8E_000618"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP0342935.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(60525..61679)
                     /gene="bioF"
                     /locus_tag="ACHK8E_000619"
     CDS             complement(60525..61679)
                     /gene="bioF"
                     /locus_tag="ACHK8E_000619"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP0342936.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(61676..62716)
                     /gene="bioB"
                     /locus_tag="ACHK8E_000620"
     CDS             complement(61676..62716)
                     /gene="bioB"
                     /locus_tag="ACHK8E_000620"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP0342937.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            62803..64092
                     /gene="bioA"
                     /locus_tag="ACHK8E_000621"
     CDS             62803..64092
                     /gene="bioA"
                     /locus_tag="ACHK8E_000621"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP0342938.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            64151..64627
                     /gene="ybhB"
                     /locus_tag="ACHK8E_000622"
     CDS             64151..64627
                     /gene="ybhB"
                     /locus_tag="ACHK8E_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP0342939.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            64779..66062
                     /gene="ybhC"
                     /locus_tag="ACHK8E_000623"
     CDS             64779..66062
                     /gene="ybhC"
                     /locus_tag="ACHK8E_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP0342940.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(66203..68464)
                     /gene="ybhJ"
                     /locus_tag="ACHK8E_000624"
     CDS             complement(66203..68464)
                     /gene="ybhJ"
                     /locus_tag="ACHK8E_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP0342941.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(68647..70080)
                     /gene="ybhI"
                     /locus_tag="ACHK8E_000625"
     CDS             complement(68647..70080)
                     /gene="ybhI"
                     /locus_tag="ACHK8E_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP0342942.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(70156..71208)
                     /gene="ybhH"
                     /locus_tag="ACHK8E_000626"
     CDS             complement(70156..71208)
                     /gene="ybhH"
                     /locus_tag="ACHK8E_000626"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP0342943.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            71392..72345
                     /gene="ybhD"
                     /locus_tag="ACHK8E_000627"
     CDS             71392..72345
                     /gene="ybhD"
                     /locus_tag="ACHK8E_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0342944.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(72386..73381)
                     /gene="pgl"
                     /locus_tag="ACHK8E_000628"
     CDS             complement(72386..73381)
                     /gene="pgl"
                     /locus_tag="ACHK8E_000628"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP0342945.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            73536..74354
                     /gene="ybhA"
                     /locus_tag="ACHK8E_000629"
     CDS             73536..74354
                     /gene="ybhA"
                     /locus_tag="ACHK8E_000629"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP0342946.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(74355..75413)
                     /gene="modC"
                     /locus_tag="ACHK8E_000630"
     CDS             complement(74355..75413)
                     /gene="modC"
                     /locus_tag="ACHK8E_000630"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP0342947.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(75416..76105)
                     /gene="modB"
                     /locus_tag="ACHK8E_000631"
     CDS             complement(75416..76105)
                     /gene="modB"
                     /locus_tag="ACHK8E_000631"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP0342948.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(76105..76878)
                     /gene="modA"
                     /locus_tag="ACHK8E_000632"
     CDS             complement(76105..76878)
                     /gene="modA"
                     /locus_tag="ACHK8E_000632"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0342949.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(77044..77193)
                     /gene="acrZ"
                     /locus_tag="ACHK8E_000633"
     CDS             complement(77044..77193)
                     /gene="acrZ"
                     /locus_tag="ACHK8E_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP0342950.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            77322..78110
                     /gene="modE"
                     /locus_tag="ACHK8E_000634"
     CDS             77322..78110
                     /gene="modE"
                     /locus_tag="ACHK8E_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP0342951.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            78178..79650
                     /gene="modF"
                     /locus_tag="ACHK8E_000635"
     CDS             78178..79650
                     /gene="modF"
                     /locus_tag="ACHK8E_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP0342952.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            79911..80927
                     /gene="galE"
                     /locus_tag="ACHK8E_000636"
     CDS             79911..80927
                     /gene="galE"
                     /locus_tag="ACHK8E_000636"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0342953.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            80937..81983
                     /gene="galT"
                     /locus_tag="ACHK8E_000637"
     CDS             80937..81983
                     /gene="galT"
                     /locus_tag="ACHK8E_000637"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP0342954.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            81987..83135
                     /gene="galK"
                     /locus_tag="ACHK8E_000638"
     CDS             81987..83135
                     /gene="galK"
                     /locus_tag="ACHK8E_000638"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP0342955.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            83129..84169
                     /gene="galM"
                     /locus_tag="ACHK8E_000639"
     CDS             83129..84169
                     /gene="galM"
                     /locus_tag="ACHK8E_000639"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP0342956.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            84372..85124
                     /gene="gpmA"
                     /locus_tag="ACHK8E_000640"
     CDS             84372..85124
                     /gene="gpmA"
                     /locus_tag="ACHK8E_000640"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP0342957.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(85272..86324)
                     /gene="aroG"
                     /locus_tag="ACHK8E_000641"
     CDS             complement(85272..86324)
                     /gene="aroG"
                     /locus_tag="ACHK8E_000641"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP0342958.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            86640..87020
                     /gene="ybgS"
                     /locus_tag="ACHK8E_000642"
     CDS             86640..87020
                     /gene="ybgS"
                     /locus_tag="ACHK8E_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP0342959.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            87134..88075
                     /gene="zitB"
                     /locus_tag="ACHK8E_000643"
     CDS             87134..88075
                     /gene="zitB"
                     /locus_tag="ACHK8E_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP0342960.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(88072..88791)
                     /gene="pnuC"
                     /locus_tag="ACHK8E_000644"
     CDS             complement(88072..88791)
                     /gene="pnuC"
                     /locus_tag="ACHK8E_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP0342961.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(88829..89872)
                     /gene="nadA"
                     /locus_tag="ACHK8E_000645"
     CDS             complement(88829..89872)
                     /gene="nadA"
                     /locus_tag="ACHK8E_000645"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP0342962.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(90120..90195)
                     /locus_tag="ACHK8E_000646"
     tRNA            complement(90120..90195)
                     /locus_tag="ACHK8E_000646"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90160..90162),aa:Lys,seq:ttt)
     gene            complement(90229..90304)
                     /locus_tag="ACHK8E_000647"
     tRNA            complement(90229..90304)
                     /locus_tag="ACHK8E_000647"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90269..90271),aa:Lys,seq:ttt)
     gene            complement(90451..90526)
                     /locus_tag="ACHK8E_000648"
     tRNA            complement(90451..90526)
                     /locus_tag="ACHK8E_000648"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90491..90493),aa:Lys,seq:ttt)
     gene            complement(90530..90605)
                     /locus_tag="ACHK8E_000649"
     tRNA            complement(90530..90605)
                     /locus_tag="ACHK8E_000649"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90570..90572),aa:Val,seq:tac)
     gene            complement(90657..90732)
                     /locus_tag="ACHK8E_000650"
     tRNA            complement(90657..90732)
                     /locus_tag="ACHK8E_000650"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90697..90699),aa:Lys,seq:ttt)
     gene            complement(90735..90810)
                     /locus_tag="ACHK8E_000651"
     tRNA            complement(90735..90810)
                     /locus_tag="ACHK8E_000651"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90775..90777),aa:Val,seq:tac)
     gene            complement(90946..91021)
                     /locus_tag="ACHK8E_000652"
     tRNA            complement(90946..91021)
                     /locus_tag="ACHK8E_000652"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90986..90988),aa:Lys,seq:ttt)
     gene            complement(91186..91977)
                     /gene="cpoB"
                     /locus_tag="ACHK8E_000653"
     CDS             complement(91186..91977)
                     /gene="cpoB"
                     /locus_tag="ACHK8E_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP0342963.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(91987..92508)
                     /gene="pal"
                     /locus_tag="ACHK8E_000654"
     CDS             complement(91987..92508)
                     /gene="pal"
                     /locus_tag="ACHK8E_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP0342964.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(92543..93835)
                     /gene="tolB"
                     /locus_tag="ACHK8E_000655"
     CDS             complement(92543..93835)
                     /gene="tolB"
                     /locus_tag="ACHK8E_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP0342965.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(93968..95233)
                     /gene="tolA"
                     /locus_tag="ACHK8E_000656"
     CDS             complement(93968..95233)
                     /gene="tolA"
                     /locus_tag="ACHK8E_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0342966.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(95298..95726)
                     /gene="tolR"
                     /locus_tag="ACHK8E_000657"
     CDS             complement(95298..95726)
                     /gene="tolR"
                     /locus_tag="ACHK8E_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP0342967.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(95730..96422)
                     /gene="tolQ"
                     /locus_tag="ACHK8E_000658"
     CDS             complement(95730..96422)
                     /gene="tolQ"
                     /locus_tag="ACHK8E_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP0342968.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(96419..96823)
                     /gene="ybgC"
                     /locus_tag="ACHK8E_000659"
     CDS             complement(96419..96823)
                     /gene="ybgC"
                     /locus_tag="ACHK8E_000659"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0342969.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(96973..97266)
                     /gene="ybgE"
                     /locus_tag="ACHK8E_000660"
     CDS             complement(96973..97266)
                     /gene="ybgE"
                     /locus_tag="ACHK8E_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP0342970.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(97266..97379)
                     /gene="cydX"
                     /locus_tag="ACHK8E_000661"
     CDS             complement(97266..97379)
                     /gene="cydX"
                     /locus_tag="ACHK8E_000661"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP0342971.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(97394..98533)
                     /gene="cydB"
                     /locus_tag="ACHK8E_000662"
     CDS             complement(97394..98533)
                     /gene="cydB"
                     /locus_tag="ACHK8E_000662"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0342972.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(98549..100117)
                     /gene="cydA"
                     /locus_tag="ACHK8E_000663"
     CDS             complement(98549..100117)
                     /gene="cydA"
                     /locus_tag="ACHK8E_000663"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP0342973.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        24347 a        26871 c        25222 g        23976 t
ORIGIN      
        1 gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc agcagaaagt tacaacactt
       61 gcttagggca ataatttcct gtgaggtgat taccctttca agcaatattc aaacgtaact
      121 atcctttaat tttcggatcc agcgcatcgc gtaaaccatc gcccaacaaa ttgaacgcca
      181 gtacggtcag aaaaatagcc agggccggaa aaacagcgac atgcggcgcg ataaccatat
      241 ccgctcgtgc ctcattgagc attgcacccc actctggtgt cggcggctgc gcaccgaggc
      301 cgagaaatga caggctggcg gcagagataa tcgaggcgcc aatgcgcatg gtgaaaaaca
      361 ccacgataga agagacggtc ccaggcaaga tatgacgcaa caaaatggtc atatcgctgg
      421 caccaatact gcgtgctgac tcgataaagg tttgctgttt caacaccagc gtgttgccgc
      481 gcaccagccg ggcaaacgcg gggatggaaa aaatggcgac tgcgataatc acgttagcaa
      541 tgccgcttcc caacaccgca acaacagcga tcgccagtaa aatacccggg aaggcaaaca
      601 acacatcgca aatgcgcatg atcagccgat cccaccagcc ttcataatat ccagccagca
      661 agcccagcaa cgtcccgatc gccgcaccga taaacacggc aaacacgccc gccgccagcg
      721 atatttgcgc accaaccagg acacggctga aaatgtcgcg ccccagtgaa tcgacaccaa
      781 accagtgctg caaagaaggt ccgttattca gattgtcata atcaaaataa ttttcggcgt
      841 catagggagc gatccagcgt gcaaaaatgg ccaccacaat caataaaata acgaacaatg
      901 cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca gaattcatgc cacggtgtac
      961 gtacctggtc aggtttgacc agtggcatgg cgtttaacac cgcctgacgt cgccagttaa
     1021 atagtcgcat ccttacttgt acctgatagc cgggttaatg gcggcgtaaa gcacatccac
     1081 cactaagttg ataagaataa attccagcga gaaaagcaga atttccgcct gaatcaccgg
     1141 gtaatcgcgc atttcaacgg agtcaacgag taagcgtcca agtccgggcc agttgaaaac
     1201 tttctccacc acgatggaac caccgagtaa aaatccaaac tgtaagccca tcatggtcac
     1261 taccgggatc atcgcgttgc gtagcccgtg tttgaggaca acccaggttt cgctcacccc
     1321 tttcgccctc gcggtacgca tataatcttc gcttaaaacg tcgacaaacg acgcgcgggt
     1381 aaagcgcgcc atcacggcgg ccactgccgc gccgagcgtc agggagggta aaatgtagtg
     1441 ctgccaactg tctgctccca cggtaggcag ccagcccagt tcaacagaga atacctgaat
     1501 taaaagcatc cccagagcaa atgccggaaa cgaaatcccc gacaccgcaa tagtcatact
     1561 caatcgatcc ggccaacggt tacgccagac ggcggcgata attcccgccg ccataccaaa
     1621 tatcaccgcc cagaccatac tggttatggt cagccacagc gttggcataa agcggctggc
     1681 aatctcatcg ccaaccggac gacgcgacac catcgacagg ccaaaatccc cctgcacagc
     1741 attgctgata tagtgccaga actggtgata cagcggctga tccaacccca gctgctgacg
     1801 caccagttct ataacctgcg catcagcttc gggcccggca attaatcgcg ccggatcgcc
     1861 gggcagcatg tggacaaata aaaacaccag caccgagacg ataaacagcg tcggaatcaa
     1921 ccccagtaag cgtttgataa cgtaattaag cattccactc cctgcgttgc ttattgcaaa
     1981 tccgcatctt caaagctgaa gccggtgtct ggcatgatcc aaaaaccggt caggttttta
     2041 ctgtgtgccg acacaagttt ttctaccacc agcgggatcc acggcgactc ttgccagatg
     2101 atatcctgcg ccgccttata caagcgggtc ttttccgccg gatcattagt tttcagtgcc
     2161 tgagccagga agtcatccac ctgtttattg ctgtaaaacg cggtattaaa cagcgttggt
     2221 ggccagttct gcgaggcaaa cagcggcgat agagcccagt cggcttcgcc ggttgaagcc
     2281 gaccagccag tgtagaacat ccgcacgccg ctctcttttt gccctttacc ttcaacttct
     2341 gccgcccgct gtccggcatc catcgcagtc acctgggctt taatcccaac ctgcgctaac
     2401 tgctgctggg taaattgcag cactttctgc gcggtgctgt ggttatgtga cgaccacagc
     2461 gtggtactga aaccgttggg atatcccgcc tctttcagta attcgcgcgc tttcactgga
     2521 tcgtaaggcc acggtttata actttgcgcg taggcgatac tcggcggtac cacaccagta
     2581 gctggcgttg catagcccgc gaaggccact ttcaccaacg ccgggcggtt aatggcgtaa
     2641 ttcagcgcct cacggacctt cgggttatcg aacggctttt gcgtcacgtt catactgata
     2701 taacgctgca taattgacgg actggccatc aactcgatat ttttgttttt ctccagcagt
     2761 gcggcctgct cgtagggaat ggggaaagca aactgcgctt caccggtttg cagcattgcc
     2821 gcgcgggtgt tgttatccgc caccggacgc caggttatgc tgtccagttt gggcaatcct
     2881 ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat cggtctgatt ccaggtatcc
     2941 agttcatacg gcccggttcc caccggatga aaaccaatct ccttgccata tttttccagc
     3001 gctgccggtg aaatcatcgc ggtcgccgga tgggcaagaa tattaataaa cgctgagaat
     3061 ggctgtttga gggtaatctt taccgttgtc ggatcgatcg cttccgtttt ggcaatattc
     3121 ttatacaggt tatagcgttt aagatgattc gccggatcgc tggcccggtc cagattcgct
     3181 tttaccgccg ctgcgttgaa gtcggttcca tcctggaatt taatgccttc ccgcagtttc
     3241 acggtgtaag taaggccgtc atcggaaacg gtataactct ccgccagcac gtttttcagt
     3301 ttcatctctt tatccagacc gaacagcccc tggtaaaacg atttcgctac ggcctgagat
     3361 aacgtgtcat tcgcgtcata tggatcgagc gtggtgaagt tcgatcctac cgccaccacc
     3421 acatctttgg cagcgaatgc acaagatgcc atcaacgctg tcgcaatgcc cagcgccact
     3481 aacccactac ggtgtacagc tcttgccatt ttattctccg cctgaatgtt atctacgcat
     3541 gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc ggcccgacgc attgcaacga
     3601 gacggctaca acctcttcac cacgcagatg aatattgctg ggaagatcgt ccgacagcag
     3661 tacacgctgc ggtcgttgtc gggacggttc agcgaccgga actgccgcca gtaatttacg
     3721 cgtataagga tgctgcgggt tttcgaagac cgcgcgccgt ggaccaattt caacaatttg
     3781 tccgcgatac atcaccgcca cacgatgact aatccgctct accacggcca tatcgtggga
     3841 gataaacaga tacgcaatgc cgaaatcacg ctggagatcg agcaacaagt tgataatctg
     3901 cccgcgaata gaaacatcca gcgccgagac ggcttcgtcg gcaatgatca cttttggatt
     3961 caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga ccgccggaaa actcatgagg
     4021 gtaacgccag gcatgttccg gtaacaggcc cacgcgctcc agcaaccacg caacgcgtgc
     4081 agccgcttct ttacctggca ataaaccgtg tacgcgcagc ggttcgataa tcgaatcacc
     4141 gatggtctga cgtgggtcca gcgaagcgta agggtcctga aaaataaact gaatatcccg
     4201 gcgcaaagcc tgaagtttac cggatgacaa ggtatcgatt cgctgaccgt taaagataat
     4261 ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc ccggtagtgg atttaccgct
     4321 gccagactcg cccaccagcg atagcgtttc gccaggccag agatcaaaac tgactttctc
     4381 aacggcatgc acttcccgcg ttacgcgatt caacaaaccg ctgcgcaaag ggaaacgggt
     4441 gaccagatta cgcacccgta aaacaggttc gccatccacc accgttttct gctcgatggg
     4501 gggttcctgt tttgctggat gttcaagcga tatcaatggg aaacgtcggg gataatctaa
     4561 ccctttcatc gcaccaagtt gcggaacagc agctaacagc gcacgggtat aaggatgttg
     4621 cggtgcatga aaaatctgtt cgacgctacc cgtttccacc gcctcgccct gatacatcac
     4681 cagtacccga tcggcaatct ctgccaccac gcccatatcg tgagtgataa agataacgcc
     4741 catcgacatc tctttttgca atactttgat taattgcagg atctgcgcct gaatagtgac
     4801 atccaacgcg gtggttggct catcggcaat cagcaccgcc gggcggcatg acagcgccat
     4861 cgcaatcatc actcgctggc gcatcccgcc agagagttga tgcggataac gtgaaaggat
     4921 ggtttgtgcc tccggaatgc gtacctgatc cagcatccgc ttcgcctcga ccatcgcttc
     4981 ttcacgactg gcgttctgat gcagacgaat tgattcggca atctgttcac ccacagtaaa
     5041 taccgggttc agcgatgtca tcggttcctg aaatatcatc gccatatccg caccgcgcac
     5101 atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc acttcacgac tgcgccgccg
     5161 caacagcatt tgatcgcact gcactaaacc gcccgcctgt tccaacaagc gcatcaatgc
     5221 cagcgcagtc actgacttac cagagccgga ttcgccaaca attgccagcg tctcaccgcg
     5281 ttgcaggcta aaagagagat tgcggaccgc agctattttc tgctggtcct gcataaaggc
     5341 aatattcaga ttttcaaccg ccagcacatc accggcatca agttcatcac tgtgtggcaa
     5401 cggtgtcccc tttttcacgg tagataccgg tggttggcgt atcgcctgcg tagccccagg
     5461 cgcgatacat tccttcggtg ttaaacggta gcgcgacgtt accttcatgg tcgatagcga
     5521 ttaagccacc gctaccgcca agcgcaggaa gtttttccat tactacccgc tcgcaggctt
     5581 ccgcgagact taatccgccg taatccatta acgcggcgat gtcatatgcc gccagcgcgc
     5641 ggatgaagac ttcgcccgtg ccggtacaag aaaccgccac actggcgtta ttggcgtagc
     5701 atccggcacc cactaagggg ctatcgccaa ctcgtccggg taatttattg gtcattccgc
     5761 ccgtggacgt agctgccgcc agattgccgt ctaaatccaa cgccacggcc cccacggtgc
     5821 ccattttctg tttttcatcc agtggcgcac cgctatggtc gagaaccatt gccccttcct
     5881 cgcgcgctgc cagtagttgt tcataacgca aaggcgtgga gaaaatctcc ggcgagacgc
     5941 gctccatgcc atgagcaaac gcaaaatttt ctgccccttc gccaatcatc atcacatgcg
     6001 ggctttgctc catcaccagc cgggcggcaa gaaccggatt acgcagatga ctaacgcctg
     6061 ccaccgcacc ggctttcagg gtgttaccat ccatcacaca ggcgtccagt tcatgggttt
     6121 catcacgcgt aaagacagcg ccaattccgg cgttaaacag tggacactct tccagcagac
     6181 gcaccgcttc cgtcaccaca tccagcgcac tttcgcccgc ttccagcatt ttctgcccgg
     6241 tttcaacaat ggcagacagc gcctcgatgt agcgtaattc ctgttgcaga ctcatctgcg
     6301 cgcggctaat tgcacctgcg ccgccatgaa ttgcaatgac tgctttgccc atagtattcg
     6361 tccattaagg gtggctcgcc acttttgcta taaatatcag aatttttatg aattactcat
     6421 tcaatttttg aatatagaaa gatggatatg gcatgtaaag gcaggagcca acgctacgcc
     6481 cgaggtggca tacagtctgt ggcgtttttc tgacataata ggcaaattcg attttgcctc
     6541 cgcaggagtg ttttcatgga atttaccacc ggattgatgt cgctcgacac cgcgcttaat
     6601 gagatgcttt ctcgcgttac cccactgacc gcccaggaaa cgctgccact ggttcagtgt
     6661 tttggtcgta ttctggcgag cgatgtcgtt tcgccactgg atgtcccggg gtttgataac
     6721 tccgctatgg acggctacgc ggtgcgttta gccgatattg cctccgggca accgctgccc
     6781 gttgccggta aatcctttgc cggtcagccg taccatggtg aatggcctgc gggtacctgc
     6841 attcgtatta tgaccggtgc gccggtgcca gaaggctgcg aagcggtggt gatgcaggag
     6901 cagactgaac aaacggacaa tggcgtgcgt tttactgctg aagtgcgtag cgggcaaaat
     6961 attcgccgtc gcggtgaaga tatctctgca ggtgcggttg ttttcccggc ggggactcgc
     7021 ctgactaccg ctgaactgcc agtgattgct tcgctaggga ttgccgaagt tccggtgatt
     7081 cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac tccagttgcc cggtcagccg
     7141 ctgggcgacg gccaaatcta cgataccaac cgtctcgccg tacacctgat gttagaacag
     7201 ttgggatgcg aggtaatcaa cttagggatt atccgcgacg atccccatgc cctgcgcgcc
     7261 gcatttattg aagccgacag ccaggcggat gtagtgatca gttccggcgg tgtttcagtg
     7321 ggtgaagcgg attacaccaa aacgattctt gaagagctgg gggagatcgc cttctggaaa
     7381 ctggcgatta aaccgggtaa accgttcgcg ttcggtaaac tttctaatag ttggttctgc
     7441 ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct atcaactggt acagcctttg
     7501 ctggcaaaac taagcggcaa taccgccagt ggcctgcccg cgcgccagcg tgtacgcaca
     7561 gcgtcccgcc tgaaaaaaac gccaggacgc cttgatttcc agcgcggcgt gctgcaacgc
     7621 aacgccgatg gcgaactgga agtgacgacc accggacatc agggttcaca tatatttagc
     7681 tcctttagcc tcggcaactg ctttatcgtg ctggaacgcg atcgcggcaa tgtggaagtg
     7741 ggcgaatggg tggaagtaga accgtttaac gcgttgttcg gaggcctgta atggcggaac
     7801 tcagcgatca ggagatgctg cgctacaacc ggcaaatcat tctgcgcggt tttgattttg
     7861 acggtcagga agcattgaaa gattcccgcg tgctggtcgt cggccttggt gggctcggct
     7921 gcgcagcttc gcagtatctg gcaagcgccg gtgtcggcaa cctgacgctg ctcgacttcg
     7981 acacggtttc gctctccaat ctgcaacgcc agacgctgca cagtgatgcc acggtcgggc
     8041 aaccaaaggt ggaatccgcc cgtgacgccc tgacgcggat caacccacat atcgcgatta
     8101 cgccagtcaa tgcgctgctt gatgacgctg aacttgctgc gatgattgca aaacacgatc
     8161 tggtgctcga ctgtacggat aacgttgcag tacgtaatca actgaacgca ggctgttttg
     8221 ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat ggaagggcaa atcacggtct
     8281 tcacttatca ggacggcgaa ccgtgctatc actgccttag ccgtttgttt ggcgagaacg
     8341 cgttaacctg cgtagaagca ggcgtgatgg caccgttaat cggcgtaatt ggttcgttgc
     8401 aggcaatgga agcaatcaaa ctgctggcag gttatggcaa accagccagc ggaaaaatcg
     8461 tgatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa actgatgcgc aatccggggt
     8521 gtggggtgtg cgggcagtaa ctgccacctc caggccagat aagacgcggc actccgccac
     8581 gtcatcagca atttatgagg atgcagaacg tccggttaaa tcgacgttct gccaaacgct
     8641 cccagccagt cctgttcaaa cttcgccaca gcggcatcaa ccgccggata gctaatcatc
     8701 tgttgtgcca catccagcgg cagagtaatt gattcacatc ctgccagtaa gcagtccagc
     8761 gcctgacgcg gggttttgaa actcgcggct aacactttcg cctgcggcgc atgcattttc
     8821 aataactggt gtaagtcggt cacagtctga ataccgctac cgccctgagc atcaatacga
     8881 ttaacgtaag gcgcaacata ttccgcacct gccagcgccg acagtagccc ttgtgctgcg
     8941 ccatataccg cggttcccag cgtcggaatc ccttccgctt ttaacatctt aatagctgcc
     9001 agcccctcag cggtcaccgg aaccttcacc acgatatccg caataataga acgcaattta
     9061 cgtgcgtcat taaccatccc ttcagcagtg gtagccatta cctgggcaaa cagacgcccc
     9121 tgaccgccca tcgcctcgtg aagttgcgga agcacaacct ccagcggttt tttacccgct
     9181 gcgataatgc ttgggttagt ggtcacaccc gccagcggaa aaatacgtga cagcgtcttc
     9241 accgcaacaa cgtctgaagt atccagatac agttccataa ccatcctcaa aatgtacttt
     9301 ttgtctgaat gtagactatc acgggaaaac tctctgaaca gttgacgcac atcaatctaa
     9361 ctttcattcg aaagtatttt aatgtttgaa cgaaacagag aggcaactat gattttcaat
     9421 attcaacgct actcgaccca tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc
     9481 tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg
     9541 ctgtatgacc cacgactctg tctggaaggc tgtgagctgt gcgctaagac cgcaccggaa
     9601 gtgattgaac gcgcgttgaa tggtttgctt attcatcggg aaaagttaac cccggagcat
     9661 tttacggcgc taaccgactg ctgcccgaca caggcgttaa ccgtgtgtgg tgaagtgaaa
     9721 agcgttgagg agatcatggc aaccgttttg cgcgataaac cgttttacga tcgcagcggc
     9781 ggcggcttaa cgctttctgg cggtgaaccg tttatgcagc cggaaatggc gacggcgctg
     9841 ctgcaagcca gccacgaggc cggcattcat actgcggtag aaacctgtct gcatgtgccg
     9901 tggaaatata tcgccccttc tctgccctat atcgatctgt ttcttgccga tttaaaacac
     9961 gttgccgacg cgccgtttaa acagtggacc gacggtaacg ccgccagggt gctggataac
    10021 ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc gcgtgccact gattcagggc
    10081 tttaatgccg acgaaacctc tgtgaaagcc attaccgatt ttgccgccga cgagctgcac
    10141 gttagcgaaa ttcatttcct gccctaccac acgctgggca tcaacaaata tcacttactt
    10201 aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc cagaactgct cgactttgcc
    10261 cagcagtatg cctgccagaa agggttaacc gcgaccttac gaggataaca atcatgacaa
    10321 cactaaaact ggatacgctc agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata
    10381 ttgtgaaacc gccggtctgt accgaacgag cacaacacta taccgagatg tatcaacaac
    10441 atctcgataa gccaatcccg gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc
    10501 gcaccatctg gatcaaacat gatgaattga tcattggcaa ccaggcaagc gaagttcgcg
    10561 ccgcgccgat cttcccggaa tataccgtct cctggatcga aaaagagatt gatgatctgg
    10621 cagatcgtcc cggtgctggt tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaag
    10681 tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg ctacggcatg tttaccgatg
    10741 agcaaaaagg tctgctggcg accggcatca ttaaagcgga gggcaatatg acctccggcg
    10801 atgcgcacct ggcggtcaat ttcccgctac tgctggaaaa aggtcttgat ggtctgcgtg
    10861 agaaagtggc ggaacgtcgc tcgcgcatca atctgacggt tctggaagat ctgcacggcg
    10921 agcaattcct gaaagcgatt gatatcgtgc tggtggcagt cagtgaacac attgaacgtt
    10981 tcgctgccct ggcgcgtgaa atggccgcaa ccgaaacccg cgaaagccgt cgcgatgaac
    11041 tgctgacgat agcagaaaac tgcgatctta tcgcccacca gccgccgcag actttctggc
    11101 aggcgctgca actgtgctac ttcatccagt tgattttgca gatcgaatct aacggtcact
    11161 cggtatcgtt tggtcgtatg gaccagtatc tctacccata ctatcgccgt gacgttgaac
    11221 tgaaccagac actggatcgc gaacacgcca tcgagatgct gcatagctgc tggctgaaac
    11281 tgctggaagt gaacaaaatc cgctccggct cacactcaaa agcctctgcg ggaagtccgc
    11341 tgtatcagaa cgtcaccatt ggcgggcaaa atctggttga tggtcaaccg atggacgcgg
    11401 taaatccact ctcttatgcg atcctcgaat cttgcggtcg cctgcgttcg actcagccta
    11461 acctcagcgt gcgttaccat gcgggtatga gtaacgattt cctcgacgcc tgtgttcagg
    11521 tgatccgctg cggcttcggg atgccggcgt tcaacaacga cgaaatcgtg atcccggaat
    11581 ttattaaact cggtattgaa ccgcaggacg cttacgacta cgcagcaatt ggttgtatcg
    11641 aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg catgagcttt atcaacttcg
    11701 cccgcgtgat gctggcggcg ctggaaggtg gtcgtgatgc caccagcggc aaagtgttcc
    11761 tgccacaagc aaaagcgttg tcagcgggta acttcaacaa cttcgatgaa gtaatggacg
    11821 catgggatac gcaaatccgt tactacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg
    11881 ataccatgct ggaagagaac gtacacgata ttctctgctc ggcgctggtc gatgactgta
    11941 ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc
    12001 aggtcggcat tgctaacctc ggcaacagcc tggcggcagt gaagaaactg gtatttgaac
    12061 agggcgcgat tggtcagcaa cagctggctg ccgcactggc ggatgacttc gacggcctga
    12121 ctcacgagca gctgcgtcag cggctgatta acggtgcgcc gaagtacggc aacgacgatg
    12181 atactgtcga tacgctgctg gctcgcgctt atcagaccta tatcgacgaa ctgaagcagt
    12241 accataaccc gcgctacggt cgtggtccga ttggcggcaa ctattatgcg ggcacatcgt
    12301 ctatctccgc taacgtaccg tttggcgcgc agacgatggc aacgccggac ggacgtaaag
    12361 cacatacccc gctggcagaa ggcgctagtc cggcctccgg tactgaccat ctcggcccta
    12421 ctgcggtcat tggctcggtg ggtaaactgc ctacggcagc aattctcggc ggcgtgctgc
    12481 tcaaccagaa actgaatccg gcaacgctgg agaatgaatc tgacaagcag aaactgatga
    12541 tcctgctgcg taccttcttt gaagtgcata aaggctggca tattcagtac aacattgttt
    12601 cccgcgaaac attgctggag gcgaagaaac atccggatca ataccgcgat ctggtggtac
    12661 gcgtagcggg ttattccgcc ttcttcaccg cgctctctcc agacgcacag gacgatatca
    12721 tcgcccgtac cgaacacatg ctgtaatccc ctcagcccgg cgtcgtcgtc gccgggccaa
    12781 atcattcaca tcatcaattt cagcctcgct ttcattcgaa atataatttg tgctctgcgt
    12841 cacattgtta ttacactgtt acggtttcat tttcgagcca ggagcacacc tatgagcgta
    12901 aaagttatcg tcacagacat ggacggtact tttcttaacg acgccaaaac gtacaaccaa
    12961 ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg gcattgagtt cgttgtcgcc
    13021 agcggtaatc agtattacca gcttatttcg ttctttcctg agctaaagga tgagatctct
    13081 tttgtcgcgg aaaacggcgc actggtttac gaacatggca agcagttgtt ccacggcgaa
    13141 ctgacccgac atgaatcgcg gattgttatt ggcgagttgc taaaagataa gcaactcaat
    13201 tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa atgcccccga agcattcgtc
    13261 gcactgatgg caaaacacta ccatcgcctg aaacctgtaa aagattatca ggagattgac
    13321 gacgtactgt tcaagttttc gctcaacctg ccggatgaac aaatcccgtt agtgatcgac
    13381 aaactgcaca tagcgctgga tggcattatg aaacccgtca ccagtggttt tggctttatc
    13441 gacttgatta ttcccggtct acataaagca aacggtattt cgcggttact gaaacgctgg
    13501 gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg
    13561 aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc
    13621 cgttacgcta ccgatgataa taatcatgaa ggcgcgctga atgtgattca ggcggtgctg
    13681 gataatatcg ctcctttcaa tagttgatct tccccctggc cgaagtgacc acttcggcct
    13741 ctctcctttt tgctcgcttt gtgcactgac tcaatatttt tatcttgcgt taacttattt
    13801 ttaacttaat gtatcaatcg caatcatttt actttcgaat gaaagaagcg aggttgatat
    13861 gactttttcc tgtgagacgc tgcccacaga tgcgaaagca gctatccgag agatgaaagc
    13921 tgcgctgcgg gaacagctca gtgatgtgca acaggttttc gatacactct ctgcaaaaat
    13981 cgaaacgcgt gttgctgaaa tcgatgccct aaaagcgcaa gggctgccgg tttggccgga
    14041 agtttcttat agtgatattg ccgcaggtac agtaagcgat gccactcgca atgaaattaa
    14101 acgtcgcggt tgtgcagtca taaaaggcca tttccctcgc gagcaggcga tggcgtggga
    14161 tcgtgccatg ctcgattatc ttgatatcaa tcattttgat gatgtttata aaggccctgg
    14221 cgacagtttt ttcggctcgc tggaggcttc tcgtcccgaa atctatccca tttactggtc
    14281 gcaagcacag atgcaggcgc ggcagagcga aaacatggct gctgtgcaat cttttctaaa
    14341 ccgactgtgg aagtctgaaa gcaacggcaa acagtggttt aatcctgata tcagtgtgat
    14401 ttatccagac aggatccgcc gccgccctcc aggtactaca tctaaaggcc tgggcgcgca
    14461 taccgattct ggtgcgctgg aacggtggtt attacccgct tatcagcgcg tttttgccaa
    14521 tgtgtttaat ggtaatttcg atgattacga tccctgggat gctgcacacc ggaccgatgt
    14581 cgaagagtac acggtagata acactacaaa atgttcggtg ttccgcacgt ttcagggctg
    14641 gacggcgctc tctgatatgt tatcgggtca gggattgtta catgtggtgc ctatcccgga
    14701 agcgatggcg tatgttttat tgcgcccgct gctggatgac gtaccggacg atgaactgtg
    14761 cggggttgca cctggcaaag ttctgcccat ttcagagaaa tggcatccac tactgcttaa
    14821 agcgctgact tccattcccg cgataacagc aggggattcc gtctggtggc actgcgatgt
    14881 gattcactcc gtcgcgccag tagacaatca acagggttgg ggcaacgtga tgtatatccc
    14941 tgctgcgcca atgtgcgaga aaaatcgggc ttatgcacac aaagtcaaaa tggcgctgga
    15001 aaaaggcgca tcacctggcg acttcccgcg ggaagattat gaagcaaact gggaaggccg
    15061 ctttacgctg gaggatctga acattcacgg taagcgcgca ctgggcatgg atgtttgaga
    15121 gttaatggaa gtgaattgat gtgggattaa gaaaatcgat tcaacgttac tcagattgct
    15181 gacaaatgcg ctttgttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc
    15241 ctgcagattc aatatattgc aggtactgcg taggcctgat aagcatagcg catcaggcaa
    15301 tcttgcgttt gtcatcagcc gccgtcaccc cgacagtatc gggctgacgg ataataactt
    15361 actcgatccc tttactacgc agataatctt cgtaattacc gctaaagtcg atcacgcgtt
    15421 ccggggtgat ttccagaatt cgggtcgcca gggagcttac aaactcacgg tcgtgagaca
    15481 caaagatcag cgtgccctga tacagttcca gcgccatgtt cagcgactca atggattcca
    15541 tatccaggtg gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact
    15601 taccaaacag catccgccct ttctccccac cggaaagcac tttcgctggc tttttgatgt
    15661 cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt
    15721 cctgcttcca ctgactcatc cattcgaaca cggtaagatc attttcgaac tcatattcgt
    15781 ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg
    15841 gttgcagatc gcccaccagc gttttcaaca gtgttgattt accgacgccg ttggtaccca
    15901 gtaccgccag tttttcaccg acttccagca gcaggttgag atttttaaac agcggaccgt
    15961 tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct
    16021 gttcaaaacg gatgaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt
    16081 tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcactaaagc
    16141 ggctaacgaa agattgcagc tcagcaatct gcgctttctt cttggcgtta tcagccagca
    16201 gacgctcacg cgcctgggtc gccgccgtca tgtactcatc gtagttacct ggataaacgc
    16261 gcagctcgcc gtaatccaga tccgccatgt gggtacaaac catgttaagg aagtgacggt
    16321 cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccaacgaa
    16381 tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag gagaatgtcc ggatcagcaa
    16441 acagcgcctg cgccagcagc acacgcagct tccagccagg agcaacttca ctcatcgggc
    16501 cgtagtgctg ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg
    16561 cagagtaacc gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt
    16621 cttcactcat ttccggcaaa gcatagatgc ggtcacgctc ctgcttcact tcccacaact
    16681 ctttatgccc aatgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct
    16741 gacgcagctt accaatgcgc tcgttgggat cgagggaaac gttacccaac gtcggctcta
    16801 agtcgccgcc gaggatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc
    16861 cgtaacggtt gccgccgcca aatttgacgg aaatgttttc aaacaacggc ttactgccga
    16921 actgcatggt gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa
    16981 ccgcgcatta tgccataaat ctgaaagaag atcctgctcc tcgcttaagg tctacactca
    17041 agccaaaggc aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac
    17101 ttataatgcg cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc
    17161 tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc
    17221 ttttgcagta ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt
    17281 ggtcagaatc aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc
    17341 gccgagtacc agatgggact ttccaatatg atggaagcga acccgggtgt ggataccttc
    17401 ctgccgaaag gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt
    17461 catgaaggca tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaaggaacc
    17521 aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag gcaaagacac gcctatcaac
    17581 tggaccacca aagttgagcg taaaaaagca ggcccgacct ggacgccgac cgccaaaatg
    17641 cacgcagagt accgcgctgc gggcgaaccg cttccggctg tcgttccggc aggtccggat
    17701 aacccgatgg gtctgtatgc actctacatc ggtcgcctgt acgctatcca tggcaccaac
    17761 gccaacttcg gtatcggcct gcgtgtaagt catggctgtg tgcgtctgcg taacgaagac
    17821 attaaattcc tgttcgagaa agtgcctgtc ggtactcgcg tgcagtttat tgatgagccg
    17881 gtgaaagcga ctaccgaacc agacggcagc cgttacattg aagtccataa cccgctgtct
    17941 accaccgaag cccagtttga aggtcaggaa attgtgccaa ttaccctgac gaagagcgtg
    18001 cagacagtga ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat
    18061 cgttccggga tgccggttcg tctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca
    18121 taatccgcct tttttatttg ccagacccta ccaggccggg atcataacca acaaaacata
    18181 tcccaccagc gccgcccaca acagcatcgg tatcgaataa agatgaaaac gccaccagat
    18241 acggcgatcg ttcgccatcc gtaacgcaat caaatttgcc agcgatccgg gtaataaacc
    18301 aaagccccct acgtttaccg cccatgccag cagcaacgac ggcggcacat agttcagcaa
    18361 caatatggta ctcggcacgt tactgatcac ctgcgataaa ccgattgccg ttaaccataa
    18421 tccgggttcg gataaatgac tcacgttact taacaccccc tgcaaagctg gcagttgggt
    18481 tagtaaatgg acgtcgataa acatcgccat aaacaccagc agcagcgtcc agtccacact
    18541 gagcaccacg cgacgtgcca gcagcgcaaa gcccaccgcc acaatcacca gtccccacag
    18601 ctcttgtttt aactccagcg ccatcagaaa gacgatatac agccccagac aactccacac
    18661 cagccgcggt ttccactccg gcgtttgcgc ccccgtatgg tattgcagcg cctttccagg
    18721 gaaacaacac cagcacaaca gcaggagtgt cagcatcatt gcgccagcca gcggtgccat
    18781 ttgggcaata aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg
    18841 gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag
    18901 ccgattaacc gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa
    18961 cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat
    19021 cgccagccga cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata
    19081 accgcttaac tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg
    19141 ccagtcgata gcagcaggcc aggatttcgg tgcaaaggga acgaaaaaac ttaatccgat
    19201 gccaacaaga attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag
    19261 gctcatttgg caccgtgttt ctgggtaaac aaacgaaagg catccagcgt ctcttcacta
    19321 acatggtgct ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc
    19381 aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag tttctctcct
    19441 tctgccgtta aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagt
    19501 cgcttaagca ttttagccac cgtcggttgc gaaactccca aacgagcagc catgtatacc
    19561 tgacgagctt cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaatc
    19621 agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc
    19681 actaactgcg tcactttttt tgctgttggc gtacctgcgc gacgactcat tgtgcttcct
    19741 cattatggtg acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc
    19801 gaaggtacaa aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg
    19861 tatgtaatgc aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa
    19921 tgagttcaag aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct
    19981 gctctctatg ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa
    20041 atagcggcgt cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg
    20101 tctgaaaaac cccacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca
    20161 gtagttgacc tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc
    20221 gcggatgcgg acccctttcc cctctccacg cactcttgca ggtattgacc cttgacgcca
    20281 aggtaagcac atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc
    20341 ttgttacccg tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat
    20401 taattaacct cggcttaggt tatcccttca gtttgctcta caccgctgcg tttacggcta
    20461 ttttgctttt gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt
    20521 cgctggtggc ggcttgttat ttcccttttg ctcaggccta cggcgcacct aatttcaata
    20581 cattgctggc attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc
    20641 cgtggtacag ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc
    20701 ggaaaaaaga gagtgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca
    20761 gtgtggcgac attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac
    20821 tgaaagatac tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg
    20881 aggtgattga agagcaagaa cggatggcaa aactgtccgg aatgaaagat acctggacgg
    20941 tcactgccgt taagccgaag tatcagacct atgtggtggt gatcggagaa agcgcgcgtc
    21001 gcgatgccct cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta
    21061 acggtttgat atttgctgac tacattgccg ccagtggctc cacgcagaaa tcgcttggct
    21121 taacgctcaa tcgcgttgtc gatggcaaac cacagtttca ggataacttt gtcaccctgg
    21181 caaatcgcgc gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg
    21241 ataccgccat cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta
    21301 attttgaagc agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc
    21361 tggcgcaaga gcactcgcaa ccgcagctga ttgttttaca tctgatgggc tcacatccgc
    21421 aggcctgcga ccggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt
    21481 gctatctcta taccatgacg caaacagacg atttactgcg taagctgtac gatcagttac
    21541 gcaacagcgg cagtagcttc tcgctggttt acttttctga ccacggtctg gcctttaaag
    21601 agcgtggcaa agacgtgcaa taccttgccc atgatgataa gtatcagcaa aatttccagg
    21661 tgccttttat ggtcatttcg agcgacgata aagcgcatcg ggtgattaaa gcccgccgct
    21721 ctgccaatga cttcttaggc tttttctccc agtggacggg aattaaagcg aaggaaataa
    21781 atatcaaata cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc
    21841 agttacagaa ggtagattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa
    21901 aacaaaaatc cgccccgaga ggcggatttt ttatatcacc gaagtgatta gaagcggtaa
    21961 ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag
    22021 aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg
    22081 tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc
    22141 acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg
    22201 atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg
    22261 taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg
    22321 ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt
    22381 acggtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct
    22441 gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt
    22501 taaatatatc aaaaattaat gggaaactct tcgcgatttg tgatgtctaa cgggccattt
    22561 catgtaacag aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag
    22621 agctgcataa gtattacgca tgttttccct gtcattcatc cagattattc ctaatcacca
    22681 gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa ttttttcaac
    22741 aaatgctcaa ccagcattgg gtatatccag tacactccac gctttactta ggtttagata
    22801 tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt accaatagtc
    22861 atattgctcg ttgccatggc gtctattcag ggtggagcct cgttagctaa gtcacttttt
    22921 cctctggtgg gcgcaccggg tgtcactgcg ctgcgtctgg cattaggaac gctgatcctc
    22981 atcgcgttct ttaagccatg gcgactgcgc tttgccaaag agcagcggtt accgctgttg
    23041 ttttacggcg tttcgctggg ggggatgaat tatctttttt atctttctat tcagacggta
    23101 ccgctgggta ttgcggtggc gctggagttc accggaccac tggcggtggc gctgttctct
    23161 tctcgtcgcc cggtagattt cgtctgggtt gttctggcgg ttcttggtct gtggttcctg
    23221 ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct ggcactggga
    23281 gcaggggctt gttgggctat ttacatttta agtgggcaac gcgcaggagc ggaacatggc
    23341 cctgcgacgg tggcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc aattggagcg
    23401 cttcaggctg gtgaagcact ctggcactgg tcggttattc cattgggtct ggcggtcgct
    23461 attctctcga ccgctctgcc ttattcgctg gaaatgattg ccctcacccg tttgccaacg
    23521 cggacatttg gtacgctgat gagcatggaa ccggcactgg ctgccgtttc cgggatgatt
    23581 ttcctcggag aaacactgac acccatacag ctgctggcgc tcggcgcaat tatcgccgct
    23641 tcaatgggtt ctacgctgac agtacgcaaa gagagcaaaa taaaagaatt agacattaat
    23701 taaatttaca tttctgcatg gttatgcata accatgcaga atttatcgct acttttcctc
    23761 tacaccgtct ttatatatcg aattatgcaa aagcatattt attccgaaaa ttcctggcga
    23821 gcaaataaat aagaattgtt cttattaata tatctaactc attgagtttt tattggtttt
    23881 gttttttcac gcttgttacc actattagtg tgataggaac agccagaata gcggaacaca
    23941 tagctggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga ggatatgaaa
    24001 ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat acccgcaacg
    24061 atgtctctga tagcgagaaa aaagcaacag tagagttgct gaatcgccag gttatccagt
    24121 ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc gctaacttca
    24181 ttgccgtaca tgaaatgctg gatggcttcc gcactgcact gatcgatcat ctggatacca
    24241 tggcagaacg tgcagtgcag ctgggcggtg tagctctggg gaccactcaa gttattaaca
    24301 gcaaaactcc gctgaaaagt tacccgctgg acatccacaa cgttcaggat cacctgaaag
    24361 agctagctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga
    24421 aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat aaattcctgt
    24481 ggtttatcga atctaacatc gaataaatcc atcgctgatg gtgcagaact ttagtacacg
    24541 ataaaggcgg cttcctgaca ggaggccgtt ttgttttgta gcccgcctca acgcacttat
    24601 ttagtgcatc catctgctat cttcagctga ttaagtaaat tttttgtatc cacatcatca
    24661 cacaatcgtt acataaagat tgttttttca tcaggtttta cgctaaataa tcactgtgtt
    24721 gagtgcacaa ttttagcgca ccagtttggt gccccagaat ggtgcatctt cagggtattg
    24781 ccctataaat cgtgcatcac gtttttgccg catctcgaaa aatcaatgag ttgcaaaact
    24841 ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa
    24901 ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct ggcttttgcg
    24961 gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc cttcgttccg
    25021 tttgaattta aacagggcga taaatacgtg ggctttgacg ttgatctgtg ggctgccatc
    25081 gctaaagaac tgaagctgga ttacgaactg aagccgatgg atttcagtgg gatcattccg
    25141 gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac cgacgagcgt
    25201 aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt gatggtgaaa
    25261 gccaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt tgctgtgaag
    25321 agcggcactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag
    25381 ttcccgaaca tcgataacgc ctatatggaa ctgggcacca accgcgcaga cgccgttctg
    25441 cacgatacgc caaacattct gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg
    25501 gtaggtgact ctctggaagc acagcaatac ggtattgcgt tcccgaaagg tagcgacgag
    25561 ctgcgtgata aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa
    25621 atctacaaaa aatggttcgg tactgaaccg aaataataac gctacgcctg taaggcgcac
    25681 tggcagttcc ctctcccctc tggggagagg attagggtga ggggcgcaaa cccgctccgg
    25741 ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca acatatgcag
    25801 tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc
    25861 ctgtggatct cggtcctcgg tctggctggc ggcctggtga tcggattgct ggcaggtttt
    25921 gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat tgaagtgatc
    25981 cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt tcgccctgcc gatggcattt
    26041 aacgacttac gcatcgaccc atttactgcg gcggtggtca ccatcatgat caactccggc
    26101 gcatacattg cggaaatcac gcgtggtgcg gtgctgtcta ttcacaaagg ttttcgtgaa
    26161 gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat tttaccgctg
    26221 gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagcat taaagacacc
    26281 tcgctgttta ttgttatcgg cgtggcggaa ctgacccgtc aggggcaaga aattattgcc
    26341 ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct gattattacc
    26401 ctggtgctga gctttattct gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat
    26461 ttaaaaacgt ctccaagcat tttggtccaa cccaggtgct gcacaatatc gatttgaaca
    26521 ttgctcaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc
    26581 tgcgctgcat caacaaactg gaagaaatca cttccggcga tctgattgtc gatggcctga
    26641 aggttaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt atggtgttcc
    26701 agcagtttta cctcttcccg catctgacgg cgctggaaaa cgtcatgttt ggtccgctac
    26761 gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag
    26821 tcggtctggc agaacgtgca catcactacc cttccgaact ttctggtggt caacagcagc
    26881 gtgtggcgat cgcacgcgcg ctggcggtga agccgaaaat gatgctgttt gatgaaccga
    26941 cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag gatctggctg
    27001 aagaagggat gacgatggtg atcgtgacac acgaaatcgg ttttgccgag aaagtagctt
    27061 cgcgcctgat ctttatcgac aaaggccgga ttgcggaaga tggcgatccg caggtgttga
    27121 tcaagaaccc accgagccag cgcttgcagg aatttttgca gcacgtctct taacttctca
    27181 gcctcccttt cctccgaaag ggaggcacac cagattcctc tcatttaaaa tcgcccctcc
    27241 tccagcatct atacttatct ttttgctctg ttttctcact ggaggagtca tgcggtggat
    27301 cctgttcatc ctcttctgcc tgctgggcgc acctgcccac gcggtatcca tacccggcgt
    27361 tacaaccaca acgacaacgg actcaacgac tgaaccggcc ccggaaccgg atatcgaaca
    27421 aaaaaaagcg gcctatggtg cactggcgga tgtgctggat aatgacacct cacgtaaaga
    27481 gttgatcgac cagttgcgca ccgtcgccgc gacgccccct gcggaacccg taccgaagat
    27541 cgtgccgccg acgctggttg aagagcaaac cgtgctgcaa aaagtcaccg aagtcagccg
    27601 ccattatggt gaagcccttt ccgcccgctt cggacaactt tatcgcaata tcaccggctc
    27661 cccgcataag ccgtttaatc cacaaacctt cagcaatgcg ttgacccatt tttcaatgtt
    27721 agcggtatta gtgtttggtt tttactggct gattcgcctg tgcgcacttc cgctgtatcg
    27781 caaaatgggc cagtgggcgc ggcaaaaaaa tcgtgagcgc agtaactggt tgcagcttcc
    27841 ggcgatgatt atcggggcgt ttattatcga cctgctgtta ctggcactga cattatttgt
    27901 cggtcaggtt ttaagcgaca acctgaatgc gggcagtcgc accatcgctt tccaacaaag
    27961 tttgtttctc aacgcctttg ccctgattga atttttcaaa gctgtactac gcctgatttt
    28021 ttgcccaaat gtggcggagc tgcgcccgtt cacgattcat gacgagaccg cccgttactg
    28081 gagccgtcgc ctgagctggt taagcagttt gataggttat ggcctgattg tggccgtgcc
    28141 gattatctct aatcaggtga atgtacaaat tggcgcgctg gcgaacgtca tcattatgct
    28201 gtgcatgacc atctgggcgt tgtacctgat ctttcgtaat aaaaaagaga ttacccaaca
    28261 tttgctcaac tttgcggagc attcgctggc ctttttcagc ttgtttatcc gcgcctttgc
    28321 gctggtgtgg cactggctgg caagcgccta ttttatcgtg ctgtttttct tttcgttgtt
    28381 cgatccgggc aacagcctga aatttatgat gggtgcaacg gtgcgcagcc tggcgattat
    28441 tggtatcgca gcatttgttt ccggtatgtt ttcccgctgg ctggcgaaaa ccatcaccct
    28501 ctcgccacat actcagcgaa attatccgga gctgcaaaaa cggctgaatg gctggctttc
    28561 ggcggcgctg aaaacggcgc gtattctgac cgtctgcgtg gcggtaatgc tgctgttgag
    28621 cgcatgggga ttattcgatt tctggaactg gctgcaaaac ggtgcggggc agaaaaccgt
    28681 agatatcctg atccgtatcg cactcattct tttcttctcg gcggttggct ggacggtgct
    28741 cgccagtttg atcgaaaacc ggctggcttc ggatattcat ggccgcccac tacccagcgc
    28801 ccgtacgcgt accctgctga cgctgttccg taacgcgctg gcggtgatta tcagtaccat
    28861 caccatcatg attgtgttgt cggaaatcgg cgttaatatc gcgccattgc tggcaggtgc
    28921 cggggcatta ggtctggcta tctcttttgg ttcgcaaacg ctggtgaaag atattatcac
    28981 cggggtattt attcagtttg aaaacggcat gaacactgga gatttggtga ctatcgggcc
    29041 gttgaccggc actgtggaac ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg
    29101 ggcgtatcac atcattccgt ggtcttcgat aaccaccttt gctaacttcg tccgcggtat
    29161 tggttcggta gtggcaaact acgatgttga tcgccatgaa gatgctgata aagccaatca
    29221 ggcactgaaa gatgcggtag cagaattgat ggaaaacgaa gaaattcgcg ggctggttat
    29281 tggtgaaccg aactttgccg ggattgtcgg cttaagcaat accgcgttta cactgcgtgt
    29341 ttcgttcacc acgctgccac tcaaacagtg gacggtccgc tttgccctcg acagccaggt
    29401 gaaaaaacat ttcgacctgg cgggtgttcg cgcgccggtg cagacttatc aggtgctgcc
    29461 agctccgggc gcggccccgg ctgaaccgtt gccgccgggg gagccaacgc tttaacgctg
    29521 gcgattgaca aaacggcggc gctgctcgtc gttcataaag gtccaggcta taaagcggct
    29581 ctgcttttgc ccctgggcca tctctttttt taccaccttc accgcaccta cgtccgtcaa
    29641 agcacgatac aacggcggta agttttcacc acgagatacc agcgaggtaa accacatcac
    29701 ctgcttcgcg aagcctttgc tctcttcaat catctttttg ataaaagcga cctcaccgcc
    29761 ttcacaccac aactcctgtt gctgaccacc aaagttcagt gcatcgtctt tgttcagccc
    29821 caggttacgg cgtttacgtt cactacctgc ccgtgccgca gcggcggaat cgtggaatgg
    29881 cgggttacac aaggtcgcat cgtattgctc gtttttatgg atgatgccgt taaaaatcgc
    29941 cccactctct ttttgccgac gcagacgaat ggcgcggtta agccccggat tagcactgat
    30001 gatcgcctgc gcactgctta acgcctggct gctggtttca ctaccggtaa aacgccagcc
    30061 atattcatgt acgccaatca gcggataaat acagttcgca ccaacaccga tatccagaat
    30121 gctggcatta gctggaattg ttccgcttgc ttcagccagt aaatcggcaa ggtgatgaat
    30181 ataatccgcc cggcccggta ctggcgggca gagaaaaccg tccgggatat cccagttcgc
    30241 tacggcgtaa aaatgggcca gcaacgcctt attgagcgcc ttcaccgcca gcggattggc
    30301 aaagtctacg ctttgctccc cggcgggtgt aagcgtgagg aattgcctga gttcaggatt
    30361 gacctgacaa agcgtggcga gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc
    30421 cggtttctgg gcggacatag cattctcctt taagcagcgg cgtaagatac ccgcataacg
    30481 cgacagggta aatatctggc tgcgtttatc atttctaaaa accttcacat ggctatggtt
    30541 tatggtaatt caccataaaa atcatcatca tgcatgtcat tgatttatct tattttgtaa
    30601 aaatatattg ttcaaaaaat aagcgcatct ctgtcaattt tttgcgtcat tttcatcgcg
    30661 caggcgacct acacttatct ggcactaccc gttgtattta tgaggatgtc attatgaaaa
    30721 agtgcctcac actactgatt gccaccgtcc tgagcggaat ctctctcacg gcttatgccg
    30781 cgcaaccgat gagtaacctg gacagtggtc aactacggcc cgccggtacc gtttcggcaa
    30841 ctggcgcatc aaacctaagc gatctggagg ataaactggc agaaaaagcg cgcgaacaag
    30901 gcgcgaaagg ttatgtcatt aattccgcag gcggaaatga ccagatgttc ggtactgcaa
    30961 tcatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgtcca
    31021 atttgtaagc aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc
    31081 gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat tatcacttgc
    31141 acggacgcta tcacgggatt aacagtggca tcgcatccgc agagatgctt tctcgtgaca
    31201 gtgaaaattt caccatataa gaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc
    31261 ctgccagaca atttcattcg ctcacgttct ttgccggtct ttgtattggt attacgcctg
    31321 tggcgcaggc actcgctgcc gaagggcaag ctaacgcgga tgacacgctg gttgtcgaag
    31381 catcaacgcc ttcgctttat gcgccacaac aatctgccga tccgaaattc tcgcgtccgg
    31441 tagcggatac tacccgcacg atgacggtga tttctgaaca agtgattaaa gatcagggtg
    31501 caaccaacct taccgacgcg ctcaaaaacg tccccggcgt gggtgcgttt tttgcgggtg
    31561 agaacggtaa ctccaccact ggcgacgcca tttatatgcg cggtgccgat acctctaaca
    31621 gtatttatat cgatggcatt cgcgatatcg gcagcgtctc gcgcgacacc ttcaataccg
    31681 agcaggtcga agtaattaaa gggccgtccg gcaccgacta cgggcgcagc gcgccgacag
    31741 gctcgatcaa tatgatcagc aaacagccgc gcaatgattc cggcattgac gcctccgcca
    31801 gtattggcag cgcctggttc cgccgcggca cgctggacgt caatcaggtc attggtgata
    31861 ccactgcggt gcgcctgaat gtaatgggcg aaaaaacgca cgatgccgga cgcgacaaag
    31921 tcaaaaatga gcgttacggc gtcgcccctt ctgtcgcttt tggccttggt acagcgaatc
    31981 gtttgtatct taattatctg catgtcaccc agcacaacac gccagacggc ggcattccga
    32041 ctatcggttt accgggttat tctgccccat ctgcgggaac agcggctctg aatcattccg
    32101 gaaaagttga tactcataac ttttacggca cggattccga ttacgacgat tcgaccaccg
    32161 acaccgctac catgcgtttt gagcacgaca tcaacgataa caccaccatt cgcaatacta
    32221 cccgttggtc gcgcgtgaag caggattacc tgatgacggc gattatgggc ggggcgtcga
    32281 atattaccca gcccaccagc gatgtgaata gctggacctg gtcacgcacg gcgaatacta
    32341 aagatgtgag taataaaatt ctcaccaacc agaccaacct gacctcgacg ttctataccg
    32401 gcgctatcgg tcatgatgtc agtaccggcg tggaatttac ccgtgaaacg cagactaact
    32461 acggcgttaa tccggtgacg ttacctgcgg taaatattta tcatcctgac agcagcattc
    32521 atcccggcgg cctgacgcgc aacggcgcaa acgccaatgg tcagacggat accttcgcaa
    32581 tttacgcctt cgatacgctg caaatcaccc gtgattttga gctgaacggc gggatccgtc
    32641 tggataatta tcatactgaa tatgacagtg ccaccgcctg cggcggcagc ggacgcggtg
    32701 ccatcacctg ccctgctggc gtggcaaaag gttctccggt caccaccgtc gataccgcca
    32761 agtcgggcaa tctggtgaac tggaaagccg gggcgctgta tcacctgacg gaaaacggca
    32821 atgtctatat taactatgcc gtttcccagc agcctccggg cggcaacaac ttcgcccttg
    32881 cgcagtctgg cagcggtaac agtgccaacc gcaccgattt taaaccgcaa aaagccaaca
    32941 ccagcgagat tggcaccaaa tggcaggttc tggataaacg cctgttgctc accgccgcgc
    33001 tgttccgtac tgatatcaaa aatgaagttg agcaaaatga tgacggaact tactcgcaat
    33061 acggtaagaa acgcgtcgaa ggctatgaga tatccgtggc cgggaatatc actcccgcgt
    33121 ggcaggtgat tggcggctat acccagcaaa aagcaaccat caaaaacggc aaagatgttg
    33181 cccaggatgg ttcctcatcg ctgccgtata ccccggagca cgcctttacc ttatggagcc
    33241 agtatcaggc aaccgatgat atttccgttg gtgcgggcgc acgctatatc ggcagtatgc
    33301 ataaaggttc tgacggcgcg gtgggaacgc cagcgtttac cgaaggttat tgggtcgccg
    33361 acgctaaact ggggtatcgg gttaatcgca atctcgactt ccagctaaac gtctacaacc
    33421 tgtttgatac cgattacgtc gcctcaatca acaagagcgg ctaccgttat cacccgggcg
    33481 agccaagaac cttcttgctc acggccaata tgcatttctg attgagatgt ggggcgcagg
    33541 ccccactttt tggagaactt gtatgatgta ccacattccc ggcgtgctat cgccacagga
    33601 cgtcgcccgt tttcgcgaac aactggaaca agccgaatgg gtggatggac gcgtcaccac
    33661 tggcgcacaa ggtgcgcaag ttaagaacaa tcaacaggta gacacccgca gcacgttata
    33721 cgccgccctg caaaatgagg tgctgaacgc ggttaaccaa catgctttgt tctttgccgc
    33781 ggccttgccg cgtacccttt ccacgccgct gtttaatcgc tatcagaaca atgaaaccta
    33841 tggttttcat gtggatggcg cagtacgcag tcacccacaa aacggctgga tgcgcactga
    33901 cctttctgcc acgctgtttt taagcgatcc acaaagctac gatggcggcg aactggtcgt
    33961 taatgacacc ttcgggcaac atcgggtaaa acttccggca ggcgatctgg tgttgtatcc
    34021 ctccagcagc ctgcattgcg tgacacccgt aacccgcggc gtacgagtgg catcatttat
    34081 gtggatccag tcgatgatcc gcgatgataa aaagcgcgcc atgctgtttg aactggacaa
    34141 caatattcag tcgctgaaaa gccgctacgg cgaaagtgaa gagatcctgt cgctgctcaa
    34201 tctttatcat aatctgctgc gggaatggtc ggagatctga cgcttgaact gcccgagttc
    34261 gtccctatat tagctacact taactgtaca agtattgata tggggaggac gatatggcat
    34321 ccggttgggc taacgatgac gccgtcaacg aacagatcaa cagtacaatt gaagatgcga
    34381 ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct gtatgaatgt gaagagtgcg
    34441 gagcccccat cccgcaagcc cgtcgggaag ccattcctgg tgtgcgctta tgcattcatt
    34501 gtcagcagga gaaagattta caaaaaccag cttatacagg atataatcgc agaggttcga
    34561 aagacagcca gttacgttaa ctacaactgg cgggatgtat agcgagtata atttgtattt
    34621 tgcgtggcat tccgtgcctt taacgctgtc gtgactgcca cgctgcataa aacaagtcca
    34681 taagttcaac aaataaatgg attatcagta tgttagatat caatcaattt atttgaacaa
    34741 ggcggtcaat tctcttcgat tttatctctc gaaaaaaaac gtgatactca tcacatcgac
    34801 gaaacaacgt cacttataca aaaatcacct gcgagagatt aattatgaaa actatcaata
    34861 ctgttgttgc tgctatggct ctttcaactc tgtcatttgg cgtgtttgcc gcggaaccgg
    34921 taacggcatc ccaggcacag aacatgaata aaatcggcgt ggtttctgcc gatggcgcat
    34981 ccaccctcga tgccctggaa gcgaaactgg ctgagaaagc tgcggcagct ggggcgagtg
    35041 gatacagcat cacttctgcc accaacaaca ataaattaag cggtactgcg gtaatttata
    35101 agtaattcgt agaacggata aggcgtttac gctacgtcca gcacaagtat ttccctgtct
    35161 gtttgccgac agacgcatat gctctaaccc tcattgatcc tatgttaccc ttgtttgccc
    35221 gtccgccact ggacgggctt ttttttagct ggctaactgc tgacagaaag cctgcaacgt
    35281 ctcttccggc ataccaacct ttcgcgccgc atcacaaatg gcctgccagc ttcccgcatc
    35341 caatggaatc ccctgctcct gccgttcgcg acgcgtgtta acttcccact cgcccggtag
    35401 caaaatcggc ttatcgtcat catgcggcga ggctttcacc cactcggcaa aggcttcggt
    35461 ctgcgcgcta caatccggcg cgccgaacag ttccgggttg atgatgatag tggtcatgca
    35521 gttaagaatg gcatcgggac tggtttgtaa cgttttctga tgcgtcgttt taccgccgga
    35581 aagcgcccca ccaagaattt cacacatcgc tgcaagggcg tagcctttat gttcggcaaa
    35641 ggtcaacagc gaacccaacg gcgactcctg cattaccgcc ggattggtcg tcggcatgcc
    35701 gttaacgtca atcaggcaac ctggcggcac ggggacgcct ttatgccagg cgacgcgggt
    35761 tttgccaaat gcgatggcgc tggtggcgta atcaagcaac agcgggaaat tatctttacg
    35821 agggaaaacc acacagaacg gattggtgcc aaagcggctg tcgcgaccgt ggaacggcgc
    35881 gaccattgga ataccgacca cgctaacaaa gtggatagag acaaaccccg ccgctgcaca
    35941 ctgctccgcc cagtaaccga tacggccgat atgatgcgag ttatgtagcg ccacggcagc
    36001 aataccgtgc tgatgcgctt tctcaatccc cagcgccatc gcttcgtgtg ccgcgacctg
    36061 accaaatgcg cgatcgccat caagcgtaac caccgcgccc gcttctttaa cgattttggc
    36121 atggtggtta atttgcagat gcccctgact ccaggaacgc acatagcttg ggatcatgcc
    36181 aataccatgt gaatcatgcc ctgccaggtt tgccgcgatt aaatgatcgg caactaattt
    36241 cgcttcttgt tcctcgctac ccatctgacg aaatacagcc tgaataaaac tgtgcagcgt
    36301 ctgagcatca aagcgatgac cactttccat ggtttactcc tgtttatgtt atgtgtttgt
    36361 tgttttttta tgctgcacgc cgggcgcggg aaggtcaata aagatgcagg aatttcatat
    36421 tgcggtgggg taaaagccgg atgacatggc tcatccggct gagataaaaa ttaaaatgcc
    36481 tgattaatgc gcgccaggcc gtcgttgata gttgccgctt cacccgtagc catcaggcag
    36541 caagccatct ggattttcag cgattcagga atcggttcgc tgccagcaag gcaacgctta
    36601 atccactgcg ccgtggtttc cggatctttt gcttgtggca gtaactcgct accagcagtg
    36661 tcctgttttt catacagtac ccgcatccct tcacggtcaa tgagattgat ctgcgggcag
    36721 cgctgcggat tagcatacac ttcaccttca gtaccatgca ttagtagcgc ccgcccgccg
    36781 atatcactaa agaacttcgc gacgcgtcca atgtattccg gatgcgaaac gctggaaaga
    36841 cgcagtgcct caccttcggc aaatggcgtc gccagtttcg ccagggtatg tgcactgtta
    36901 cgcacgccca tccgccagcg catcgccagt tgtttttcca gcggcgggca aaacgcgccg
    36961 acaggcataa acaccggttg atgttcgtcg agcttcgcct gcgcctgccc gccgtgcaac
    37021 gttggcgtaa tacccatcag ttcgaaaatg gtttctgtca gcacgcgggt tggatcttcg
    37081 ctaaccccgt gaaccaccac aggaaaaccg agtttgtgca ggagaatcgc caacaacggc
    37141 gtcaggttgg cctgtttacg cgcgccgttg taactgggga tgacaatcgg catcggcttg
    37201 tctgcaggcg gcgtcagctt gatggtgtga ttttgcatgg cttcgtaaaa gccgagcatc
    37261 tctgcttccc cttccccttt gatacgcagc gcaatcaata cgccgcccaa ctcaaggtca
    37321 gggacttcac cattgagcat atgggcatac agaccgcgcg cagtatcccg gtctaaatcg
    37381 cgcgcgtggt ttttcccgcg cccgatctct ttaatgattt tgcgatagtc catttacgac
    37441 tccttacctg actcacatca ttaacgccgc cgacggcgtg gggatttcgt tttttctttc
    37501 tttttaacta taccttcagg gacttccggt tgctctatcg gaaataccgg taatgcatcc
    37561 agtagtcgct tgccataatt tttggtcagc aagcgtttgt cgtagataac cacttccccc
    37621 cagcaaccgt ggcttcgaat cagtcgccca acctgctgaa tcaggttaaa cgaagcgctc
    37681 ggcaggcttt gcacctcaaa cggatagcgg ttgaggcttt tcagccattc accttcggtg
    37741 atcaccaccg gactgtcgat gggcggaaaa gcgatcttgt ggatatgcac ctgactgagc
    37801 agatcgcctt tcagatcaag accttcggca aaggactgta agcccaccag cacgctacgc
    37861 tcaccgttgg caacgcgttt gcggtgcagt tcaactaagc gataacgtgg ctgatcgccc
    37921 tgcaccagca acatcaggcg taaatccgtc acatagtcga gaaagcgctg catcgcacgc
    37981 ccgctggcaa acagtaccaa cataccgaga tgttttttgc tctccacctg ctcacggaaa
    38041 aaggctgcca tttcagcaat gtgctgctct tcgttgtcga tggaaggctc aaagcgcatc
    38101 cggggaataa caattttgcc ctgttcgcag tggttaaagg gcgaatccag cgccacaaag
    38161 cggtcgcccg ctttctcttt cagaccactc atctcctgta aacgcgaaaa actgttcagc
    38221 gaacgtaagg tcgcggaagt gacaataatg tgcggaatac tgcgccacag cagcctttcc
    38281 agctgatcgc tgacacgaat acccacacag tgaaaccaga gatgcagctg cccttcgcgc
    38341 tcttcccgcg tcgcccattt agtcaccggc gcaccggaag attgcgccag agacgccagc
    38401 cgccagagtt tgctttgcgc ctcgaacatc cccaacgcgc gattcatctg caaaatcaac
    38461 cgatgcagac gtacaatgtc atggctaccg gttttctcac ttaaatcgtt aagaaataac
    38521 tccgccaggc cacgcagcat ctcggtgagt tttgccagcc gctggcagat ctccagcact
    38581 tcatcaggca gttcgcccat cgcaaaacgg tgctctgcct cctgcccggc aggcatgtag
    38641 agattgagaa tgttgtttaa cgaggcgata agctcataca gctcttcaca atgcgcattc
    38701 aaacgttcag ggatcgccag cggtgggatg gtcttcgggc gaaactgctc catgcaggta
    38761 gcgaccagtt tcgtgaacaa gtccagctgt agccgatacc agggcgcggt gatttcggcg
    38821 ctcatctcca gcgcatcccg cgccacatct ggcaggtgat gaccttcgtc cagcaccagc
    38881 agcagatttt tcgggtcagg tagtacggct tcgctttcca tcgccgccat caccagcgca
    38941 tggtttgcca ccaccacttc cgcttcctga atctcccgac gagcaacaaa aaacgggcat
    39001 tcacgatagt agtaacagtt gcggttgagg cagctggctt tgtcggtact taaacgacgc
    39061 cagagatcgt catcaatggc gatatccgta tgatcgcgca gaccatccca tttataagtg
    39121 tcgagatcgc ccttcagctt cgcacaacgt ttttgctctt cctgattgtt cggcgtcagc
    39181 tcgtcgtcaa gaaacgccag cagatcctgt tgcgtgggtt cagtactggc aagcgctgtc
    39241 agattacgcg gacaaacgta gcgcccacgc ccaaaagcag cagtgaattt aagatcggga
    39301 atgatctttt tcagcagagg caaatcttta ctgtagatct gatcctgcaa tgccacgttg
    39361 gcggtactca ccaccagcgt tttttgctct tcgcgggcaa tggcgatgcc gggaatcaaa
    39421 taggagagcg ttttcccaac gccggtgggt gcttcaatcg ccagatgccg cccttcttct
    39481 ccggccagcg ttttggcgac gtccgcaatc atctgccgct gcggcgcacg gggaataaag
    39541 tcggggatct gttcctgaag cgccttatac caggcggcaa tttgcgcttt aagcgcggcg
    39601 gttaatgcca tgagaaaacc tgaaatactg tataaacagc caatattgtg gcatttttcg
    39661 gttttcaggg gtaatggttt ttctgtttct ggaaagcggc tcgcaataac cagcaggatg
    39721 atgcgtaaag cgcttcgcgc taaattcgaa caacatgcag aactgcgtac actcttgctg
    39781 gcaaccgcgt ccgcaaaact ggttgagcat acgcaaaatg atgcttactg gggagatagc
    39841 ggtaatggtc agggcaagaa tcgcctgggg taccttttaa tggcgttgcg cggacaattg
    39901 gctgcggaga agtaacgcat cgggtggctt gtgggcgtta aaacagagaa atctttttgc
    39961 cgcgctcgca aatggctgac aagtgcttgt tgtgaaatgc cggatgcggc gtaaacgcct
    40021 tatccggcct acaaaagcat gcaaattcaa tatattacag agttcacgta ggcctgataa
    40081 gcgcagcgca tcaggcagtt ttgcgtttgt tcatcagcct gatgccgggc atcgcccggc
    40141 atcaaaagat tactgcgcag cggcaggttt acgtggacgg cggcgacgtt gttgttcgcc
    40201 tgccggtttg gcatcgccga ggcggcgaga cggtttttct gcaggtttcg cacttgcaga
    40261 ttttgcgcca ccttctccac ggcgtggttg ctgttgaccg cgaccaccac cttgcccacg
    40321 accgccgcca cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg
    40381 ctcataaccc ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg
    40441 cagcagtttg tgttcatcaa cacacaccag cgacaacgct tcaccggtag cagccgcacg
    40501 accggtacgc ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac
    40561 cacgtgcggc agctcttcaa tatccaggcc gcgcgcagcg atgtcagttg ccaccagtac
    40621 acgaatatcg cccgatttaa aatcagccag cgcacgagta cgcgcacctt gcgatttatt
    40681 accgtggatc gccgcactac ggatgccatc tttattaagc tgttcagcca gatggttagc
    40741 gccatgtttg gtacgggtaa acaccagcac ctgctgccag ttccctttcc caatcatatg
    40801 cgacagcaat tcgcgtttgc gtttcttatc gacaaagtga acgtgctggg tcacctgatc
    40861 agacgcggta ttgcggcgtg ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc
    40921 cagggcttta atatcgtcag agaaggtcgc ggagaacaac aggttctggc gcttcgctgg
    40981 cagttttgtt aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc
    41041 ttcatcaagg acgaggattt caacctgatc cagcttcact gcattttgat gttccaggtc
    41101 cagcagacgt cccggcgttg ccaccagcac atcaacgccg ccgcgcaact tcatcatttg
    41161 tgggttaatg ctgacaccgc caaacaccac cagcgaacga atgttcagat atttgctgta
    41221 atcacggacg ttttcgccaa tctgcgccgc cagttcacgg gtcggggtaa gaatgagcgc
    41281 gcgtaccgga cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg
    41341 cagcgtaaag cccgctgttt tgccggtacc ggtctgggcg ctggccatca ggtcgcggcc
    41401 ttccagcacc gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc
    41461 ggcaacggcg cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc
    41521 gaaccgcccc gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa
    41581 aaaccacaat gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta
    41641 tccctgtgac tggcgttgct aaaattctta gccatactgg acaactctca tatggagtca
    41701 taatcttaat caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca
    41761 atcaagggtg aacaggcgaa gaaacagctg attgctgccg cactggcgca gtttggtgaa
    41821 tatggaatga acgccaccac ccgcgagata gccgcccagg ccgggcagaa tatcgctgcc
    41881 atcacctatt acttcggttc gaaagaggat ttgtacctcg cctgcgccca gtggattgcc
    41941 gattttattg gcgagcagtt ccgtccacac gccgaagaag ccgaacgttt gttcgcgcaa
    42001 ccacagcctg atcgcgcagc tatccgtgaa ctgatccttc gcgcctgcag gaacatgatt
    42061 aagctgctca cccaggatga caccgtcaac ctcagcaaat ttatctcccg tgagcagctc
    42121 tctcccacgg cagcctatca cctggtgcac gaacaggtga ttagtccgct acacagccac
    42181 cttacacgcc tgattgccgc ctggaccggc tgcgacgcta gcgatacccg catgatcctt
    42241 catacccatg cgttgattgg tgagattctg gcgtttcgtc ttggcaaaga aactattctg
    42301 ttacgtaccg gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg
    42361 acctgtcata ttgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa
    42421 aacctgtcgt gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact
    42481 ggtggtatca aagccgccag gataacggcc tgacgctgta tggcaacgtg gatattcgta
    42541 cggtgaatct tagtttccgt gttggggggc gcgttgagtc gctggcggtg gacgaaggtg
    42601 atgcgatcaa agcgggccag gtactgggcg aactggatca caagccgtat gagattgccc
    42661 tgatgcaggc gaaagcgggc gtttcggtgg ctcaggcgca gtatgacctg atgcttgccg
    42721 ggtatcgaga tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagctgcct
    42781 atgactatgc gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt
    42841 cggcaaatga cctggaaaat gcccgctcct cgcgcgacca ggcgcaggcc acgctgaaat
    42901 cagcgcagga taaattgcgt cagtaccgtt ccggtaaccg cgaacaggac atcgcccagg
    42961 cgaaagccag cctcgaacaa gcgcaggcgc aactggcgca ggcggagctg aatttacagg
    43021 actcaacgtt gatagccccg tctgatggca cgctgttaac gcgcgcggtg gagcctggca
    43081 cggtgctcaa tgaaggtggc acagtcttta ccgtctcact gacacgtccg gtgtgggtac
    43141 gtgcttatgt tgatgaacgt aatctcgacc aggcccagcc ggggcgcaaa gtgctgcttt
    43201 ataccgacgg tcgcccggac aaaccgtatc acgggcagat tggtttcgtt tcgcctaccg
    43261 ctgaatttac cccgaaaact gtcgagacgc cggatctgcg taccgacctc gtctatcgcc
    43321 tgcgtattgt ggtgaccgac gccgatgatg ccttacgcca gggaatgccg gtgacggtgc
    43381 aattcggtaa cgaggcagga catgaatgat gccgttatta cgctgaacgg tctggaaaaa
    43441 cgctttccgg gcatggacaa gcccgccgtc gcgccgctcg actgtaccat tcatgctggt
    43501 tatgtaacgg ggctggtggg gccggatggc gcaggtaaaa ccacactgat gcgcatgctg
    43561 gcgggattac tgaaacccga tagcggcagc gccacggtga ttggctttga tccgatcaaa
    43621 aacgacggcg cgctgcacgc cgtgctcggc tatatgccgc agaaattcgg cctgtatgaa
    43681 gatctgacgg tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggtgaa
    43741 gcgcggaagc agacttttgc tcgcctgctg gaatttacat ctcttggccc gtttaccgga
    43801 cgcctggcgg gcaagctctc cggcggaatg aagcaaaaac ttggcctggc ctgtaccctg
    43861 gtgggcgaac cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca
    43921 cgccgcgaac tgtggcagat ggtgcatgag ctggcaggcg aaggaatgtt aattctctgg
    43981 agcacctcgt atctcgacga agccgaacag tgccgtgacg tgttactgat gaacgaaggc
    44041 gagctgctgt atcagggaga accgacggcc ctgactcaaa ctatggccgg acgcagcttt
    44101 ctgatgacca gcccgcacga gggcaaccgc aaactgttgc aacgggcatt gaaactgccg
    44161 caggtcagcg acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagagacc
    44221 acgccagacg atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg
    44281 ccgcgttttg aagatgcgtt tattgatttg ctgggcggtg ccggaacctc ggaatcgccg
    44341 ctgggcgcaa tattacatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa
    44401 gagctgacca aaaagtttgg cgattttgcc gccaccgatc acgtcaactt tgccgtcaaa
    44461 cgcggggaga tttttggttt gctggggcca aacggcgcgg gtaaatcgac cacctttaag
    44521 atgatgtgcg gtttgctggt gccgacctct ggtcaggcgc tggtgctggg catggatctg
    44581 aaagagagtt ccggtaaagc gcgccaacat ctcggctata tggcgcaaaa attttcgctc
    44641 tatggcaacc tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc
    44701 ggtcgggcgc agaacgaaaa aatctcccgt atgagcgagg cgttcggcct gaaaagtatc
    44761 gcctcccacg cgaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt
    44821 tcactgatgc atgaaccgga cattctgttt ctcgacgaac cgacgtccgg cgttgatccc
    44881 ctcacccgcc gtgaattttg gctacatatc aacagtatgg tagagaaagg cgtcacggtg
    44941 atggtcacca cccactttat ggatgaagcg gaatattgcg accgcatcgg cctggtgtac
    45001 cgcgggaaat taatcgccag cggcacaccg gacgatttga aagcgcagtc ggccaacgat
    45061 gagcaacccg atcccacgat ggagcaagcc tttattcagt tgatccacga ctgggataag
    45121 gagcatagca atgagtaatc cgatcctgtc ctggcgtcgc gtgcgggcgc tgtgcgtcaa
    45181 agagacgcgg cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccact
    45241 gctactgctg tttatttttg gatacggcat taacctcgac tccagcaagc tgcgggtcgg
    45301 gattttactg gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc
    45361 gccctacatc gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc
    45421 ggggaaaatt cgcggtctgg tggttattcc ggtggatttc gccgaacaga tggagcgcgc
    45481 caacgccacc gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt
    45541 tgtgcagggg tatgtcgaag ggatctggca gatctggcaa atgcagcgag cggaggataa
    45601 cgggcagact tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc
    45661 aataagccag cactttatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc
    45721 gattctcacc tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggcgttact
    45781 ctctacagag attacccgca cggaactgct gctgtgtaaa ctgatccctt attactttct
    45841 cgggatgctg gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta
    45901 tcgcggttcg ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg
    45961 gatggggctg ctgatttcca cgataacccg caaccagttc aatgccgctc aggtcgctct
    46021 gaacgcggct tttctgccgt cgattatgct ttccggcttt atttttcaaa tcgacagtat
    46081 gcccgcggtg atccgcgcgg tgacgtacat tattcccgct cgttatttcg tcagcaccct
    46141 gcaaagcctg ttcctcgccg ggaatattcc agtggtgctg gtggtaaacg tgctgttttt
    46201 gatcgcttcg gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga
    46261 ttagggagaa gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc
    46321 tgctgcgcga accgcaaacc cgtgcgattc ttattttacc cgtgctgatt caggtgatcc
    46381 tgttcccgtt cgccgccacg ctggaagtga ccaacgccac catcgccatc tacgatgaag
    46441 ataacggcga acactcggta gaacttactc aacgttttgc ccgcgccagc gcctttaccc
    46501 atgtgctgct gttgaaaagc ccgcaggaga tccgcccaac catcgacaca caaaaggcat
    46561 tactcctggt gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc
    46621 cgttgcagtt gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc gccaactacc
    46681 tgcaacagat cgtcaaaaat tatcagcagg agctgctgga aggaaaaccg aaacctaaca
    46741 acagcgagct ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg
    46801 tgccgtcgct gatcgccatg atcaccacta tcggcgtaat gatcgtcact tcactttctg
    46861 tcgcccgcga acgtgaacaa ggtacgctcg atcagttact ggtctcaccg ctcaccacct
    46921 ggcagatctt catcggcaag gccgtaccgg cattaattgt cgccaccttc caggccacca
    46981 ttgtgctggc gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt
    47041 tctactttac gatggtgatt tacggtttat cgctggtggg attcggtctg ttgatttcat
    47101 cactctgttc aacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca
    47161 ttctcctttc cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga
    47221 cgtggattaa ccctattcgc cactttacgg acattaccaa gcagatttat ttgaaagatg
    47281 cgagtctgga tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag
    47341 ggtcagcggc gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc
    47401 aaagacacta ccgccgggcc agcaaggatt gccagccctg caatcgccag caaaaagttg
    47461 ttttccagta cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc
    47521 gtggtcagct cttccagcac gtctgccacc tcacgccagc gttgctcggg gtggcgctga
    47581 aacaccagca tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga
    47641 accagagtct tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata
    47701 atctgccagc aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct
    47761 ctcctgaaga atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag
    47821 accgcacctt tttgtgacgc cgactacact atttcaatga gataagaggc aaagacagga
    47881 aaattatgcc cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aatattcaca
    47941 aaggctttac cgcgtttaac cgacgcttca ttttgccgga acttcgcgac gccgtgcgta
    48001 ccgtcagcgc cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc
    48061 tgcatgtgga aaactggccc gatacctcgc actacgagtt tctcgccgac accatgtgga
    48121 gcgatttcgc ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgcggttc
    48181 tttcacgtta tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa
    48241 agcgcggcgt gctctactgc cgcattgtgc cgccgatgac cgggaaagcg attcatgtga
    48301 tgtgcgtaca tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atgctcgccg
    48361 aatgggtgaa tgagctgccg gacggcgaac cagtattggt ggcgggtgat ttcaacgact
    48421 ggcggcaaaa agctaatcat ccgttaaaaa tgcaggccgg gctggatgag atatttaccc
    48481 gcgcccacgg acgcccggcg cgcacgtttc cggtgcaatt tcctctacta cgtctggaca
    48541 ggatctatgt caaaaatgcc agcgccagcg cgccaaccgc gttgccgctg cggacatggc
    48601 gacacctttc tgatcatgcc cctttaagtg cggagattca tttatgaaat gtagctggcg
    48661 cgaaggcaat aagatccagt tgctggaaaa cggcgagcaa tattatcccg cgatgtttaa
    48721 ggcgattggc gaggcgcaag aacgcatcat tcttgaaacg tttatctggt ttgaggatga
    48781 cgtcggcaaa cagctgcatg cggcgctact ggcagcagcg caacgcgggg tgaaagcgga
    48841 agtcttgctg gatggctacg gttcgccgga tctcagcgat gagtttgtca atgaactgac
    48901 ggcagctggc gtggtatttc gctactacga tccccgtcct cgcctttttg gtatgcgcac
    48961 caatgtgttt cgccggatgc atcgcaaaat tgtggtgatc gacgcgcgta tcgcctttat
    49021 tggcgggctg aattactccg ccgagcatat gtccagctac ggtccagagg ctaaacagga
    49081 ttacgcggta cgccttgaag ggccgattgt cgaagatatt ctccagtttg agctggaaaa
    49141 cctgcctgga cagagcgcgg cacgacgctg gtggcgacgt catcacaaag cggaagagaa
    49201 ccgccagccg ggagaagcgc aggtattgct ggtctggcgc gataacgaag aacatcgcga
    49261 tgatattgaa cgtcactatc tgaaaatgct cactcaggcg cggcgagaag tcattatcgc
    49321 caacgcctac ttcttccccg gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg
    49381 cggggtgcgg atcaaactga tcattcaggg cgaaccggat atgccgattg tcagagtcgg
    49441 tgcgcgcttg ctgtataact atctggttaa aggcggcgtt caggtatttg agtaccgccg
    49501 ccgcccgctc cacggcaaag tggcattgat ggacgatcac tgggcgacag tagggtccag
    49561 taatctcgat ccgctcagtt tgtcactgaa tctcgaagca aatgtcatca tccacgatcg
    49621 tcattttaac cagacgctgc gcgataatct gaacggcatt attgccgcag attgtcagca
    49681 ggtcgatgag accatgctgc cgaaacgcac ctggtggaac ctgaccaaaa gcgtgctggc
    49741 gttccacttt ttacgccact tcccggcgct ggttggctgg cttccggcac atacaccacg
    49801 tctggcgcag gttgatcccc ccgcacaacc gacaatggaa acgcaggatc gggtagaaac
    49861 tgaaaacacg ggggtaaaac cctgatgagt aaatcacacc cgcgctggcg cttagcaaag
    49921 aagatcctca cctggctgtt ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa
    49981 aaagtggact gggaagaggt ctggaaggtc atccgcgact acaatcgcgt tgcgctgctc
    50041 agtgcggtcg ggctggtggt cgtcagctat ctgatttacg gctgctatga cctgctcgcc
    50101 cgtttctact gcggtcacaa actggcgaag cgccaggtga tgctggtgtc gtttatctgc
    50161 tacgccttca acctgacgct cagtacctgg gtcggcggca taggtatgcg ctatcgtttg
    50221 tactcgcggc tagggttacc gggcagcact attacgcgga ttttctcgct cagtattacc
    50281 accaactggc tgggctatat tttgctggcc gggattatct ttaccgcagg cgtggtggag
    50341 ctgccggatc actggtatgt cgatcaaacc acgctgcgta ttctcggcat tggcttattg
    50401 atgattatcg cggtttattt gtggttttgc gctttcgcga agcaccgcca tatgaccatc
    50461 aaagggcaaa agctggtgct gccttcatgg aaattcgccc tcgcccagat gctgatttcc
    50521 agcgttaact ggatggtcat gggagcgata atctggctgt tacttggtca aagcgtgaac
    50581 tatttctttg tactgggcgt gttgctggtt agtagtattg ccggggtgat tgtgcatatt
    50641 ccggcgggga ttggtgtgct ggaagcggtg tttatcgcac tactggctgg ggagcatacc
    50701 tccaaaggta gcattatcgc cgccctactc gcttaccgtg tgctgtatta ctttatccca
    50761 ctgctgctgg cgctgatctg ttacctgcta ctggaaagcc aggcgaagaa gctgcgggcg
    50821 aaaaatgaag cggcgatgtg aagttcggtt aagttcttta gtatgtgcat ttacgactaa
    50881 tgaaaaaacc gcgtatgcct ttgccagaca agcgttatag ctatcgcggc aagtatccca
    50941 aagaagccga taaaatagta atagagtgtt aatgcacctg taaaagcgca actaaccgga
    51001 ctccacaact cttcgctatg gcattttcct gccagttcaa ggagcttatt tcgtccatgc
    51061 gagataatgc ctgaccataa aacaatcatt aagcaactta ttacccaaat gggccgttca
    51121 ctcattaatg cggcattcct gatgatcacc agggccagtc ctgcggttgt caccaaaccc
    51181 aaagcggtaa tgagccatat gccgtttaat atcctggctt cgtgctgaaa atctaattta
    51241 ttgctgcttt gcgagtatga ttccattctt ttagttatcc ttagaaaaaa tattaatatt
    51301 caccatcaat aaatttaaag ccacgcatac aaaatacttt tgtatttatg gatacggaaa
    51361 acacttccgc tcggccataa ggcatatttt atacttggta cttgttaagt gtcgtttttt
    51421 cgtaagaaaa aaatcccgac acatggccgg gatattgatg gtgaaaaaga attaacggcg
    51481 gttgccgaag atccgcaaca acatcaggaa caagttgatg aagtccagat acaaggttaa
    51541 cgcgccaaga atggaatatt tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc
    51601 catatttttc aatttctgcg tgtcatacgc cgtcaatccg acaaagacaa tcacgccaat
    51661 gtaggtaact gcccacatca atgcttcgct tttcagccag aagttgacca gcgatgccag
    51721 cacaatgccg attaacgcca taaacagcat attgccaaag ccgcttagat cgcgcttcgt
    51781 ggtgtaaccg tacaggctca ttgcgccaaa cattccggca gtaacgacga aagtactggc
    51841 gatagaagca gcggtataaa caatgaaaat actggaaagc gtaagacccg tcagcgccga
    51901 ataaagcata aagagcatcg tcgttacacc tgcgctcagc ttttgaatca tcgctgataa
    51961 cacaataacc aatgctaatt gcgcgatgat cagaccgatt aaaaagacac ggttagtgaa
    52021 caacagctcc atcacagccg cggaattagc cgcataccag gcaacaaatg cggtcagcaa
    52081 caagccaacg gtcatccagc catagacttg cgccatataa gtttgcaagc cagcccgggg
    52141 ttgtacaatt gaatcagaac gtgggaatct gtccatgacg atctcctgaa gatataagga
    52201 atatcttaag gatactgcaa aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta
    52261 ctgagcggtg aaattgccgg acgcagcggt gccttatccg gctaacaaaa aactaccagc
    52321 gttttgccgc ctgctgatcg ctctcccgag cttcaaccca gcggtcgcct tccggcgtgg
    52381 cttcacgctt ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt
    52441 caaacgcact gctgcgatgc gcactggtga caccgacaaa aacgatttca tctcccggcc
    52501 ataattcccc gatgcggtga atcaccgtga cgcgccccag cggccagcgg ttacgcgctt
    52561 catcgacaat ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg
    52621 ctttgacgct gtcgccaagg ttatggttgc gcaccttacc ggtaaaggtg actaccgcac
    52681 cgtcttcatc acgctccgcc agccacggat actcttctcc tacgctgaat ggctgcgggc
    52741 cgacaacaat tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagccact
    52801 tcgtcgccgt cagtcagcgg atggtcaaga ctcaccagcg tctggttgac ggcagccagc
    52861 aatttgccat cttccagcgc cagcgcccag cgatcgctct gcgcagccag atgttggcgt
    52921 aacgcttcaa cggttgggaa atccgcagcc acttcggttg catctgttcc caccaactcg
    52981 cgcacctggg cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt
    53041 gccgccgctc ttcgccagca aacgtaccgg accaattacc atatcttttt gcaccgcttt
    53101 gcacatgtca taaatggtca gcgccgccac ggaggccgcg gttaacgctt ccatttcgac
    53161 accggttttc ccggtcaggc ggcataaggt ttctatacgt acccgattgt gctccggctc
    53221 ggcctgtaaa ttgacttcaa ctttgctgag catcagcggg tgacacagcg ggatcagatc
    53281 ccaggtgcgt tttgccgcct gaataccggc gatacgcgca gtggcaaata cgtcgccttt
    53341 gtggtggcga ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc
    53401 cgcccgcgct tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gtgcttcgcc
    53461 agcggcgttg atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaattg
    53521 catggacgtg tacgggcatc gagctgcggc gcgatgatat tttcccacgc ggtgcggcac
    53581 gctttggtcg aacccggcat ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca
    53641 cgagattgca acgttgaggt gccaatctct tcaaacgaca acatccggaa cacttcacca
    53701 aaaccttcaa cttcacggtc gaacaacggc agcagcgctt cgggagcctg atcaccttcc
    53761 gtcaggccag taccacccgt aatcaatacc acttgtacat cgtcgctggc gatccacgca
    53821 gatacctgag cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga
    53881 tggcccgctt cctgcgccga atcgcgcaga tagtgaccgg aggtatcgtc ttcttcacca
    53941 cgacgattag aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga
    54001 ctcatctgat ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata
    54061 ccggtgttgt tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata
    54121 cgcgcttcca gcgcctgttg ctgggtatcg tcttccagca gatcgcgcag gttaacgccg
    54181 ccttcaccaa acaggcagag atggagttta ccaatggagg aaacgcgcag gcggttgcaa
    54241 gtggcgcaga agtctttttc atacggcatg ataaggccaa tctctccggc gtaatccgga
    54301 tgacaaaaga cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga
    54361 cgcagtagct cgtcacgcag aacctgacca gagatgtgat gcttacggaa gagctcgctg
    54421 ccctcacccg tttccatcaa ttcgatgaaa cgcagctgga tagggcgatg ctggatccag
    54481 ttcagaaagg tgtcgagctg atgatgatta acatcacgca tcagcacggt attgactttg
    54541 accttctcaa aaccggcctc aaatgcagca tcgatccctg ccatgacctg gttgaattta
    54601 tcctgcccgg taatggcatg aaactggcga gcgtcaaggc tatcaacgct gacgttgatg
    54661 ccagtaagtc ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg
    54721 gtgaccgcaa tctggcggat agcgtcgttt tcccgcacag cggcgatgat atcggtaaag
    54781 tcgcggcgta aagacggctc accgcctgtc aggcgcactt tttcggtgcc cagactggca
    54841 aaggcgcgcg taacccggcg aatttcatcg acggtaagaa agcctttatt ggtgacgccg
    54901 ctcggtttgt agccatccgg caggcagtag gtgcaacgaa agttacacac atcagtaatc
    54961 gacaggcgca agtagtaaaa cttacgcgca aatgcatcag tcagttgtga agccatgtac
    55021 acctttccag atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg
    55081 agcattgtca cggccaaagc accgtatcag ttgacccagg tgcagaggct agagtgttta
    55141 gtggttaagc cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgta
    55201 atcatgtata tagcgtcatg atcgtgctat ttcgctacag aataagggaa aatggcggtt
    55261 ttactttgat atacatcatc aggaaggcat agcgtacatc gtcctttaga ggtagaagaa
    55321 aatagtttgt atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact
    55381 gtagcgcgga taatctgact ccaggaaact atatgcgcaa tcgtacgctg gctgaccttg
    55441 atcgtgtcgt tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt
    55501 ctttgggttc tcgtttaacg ggtatcgtca ccaccacgga taatggcgga tcgacagggc
    55561 gtattcgccg ctcagaaggc ggtattgcct ggggcgatat gcgtaactgc ctcaaccagc
    55621 tgataactga acccagcgtc gcttccgcaa tgtttgaata tcgtttcggc ggtaatggcg
    55681 aactttccgg gcacaacctt ggaaatctga tgctgaaggc actggatcac cttagcgtgc
    55741 gacctcttga agcaatcaat ctgattcgca atctgctgaa agtggacgca catttgattc
    55801 cgatgtcgga gcatccggtg gatctgatgg cgattgacga tcaggggcat gaagtttacg
    55861 gcgaggtcaa tatcgaccag ttaactacgc cgattcagga gttactgtta acgccgaatg
    55921 tacccgcaac gcgtgaggct gttcacgcta tcagtgaagc ggatctcatc attattgggc
    55981 ctggcagttt ttacaccagc ctgatgccaa ttctgctgct gaaggaaatc gcacaggcat
    56041 tacgccgcac gccagcgccg atggtctata taggcaatct ggggcgtgag ttgagtttac
    56101 ctgcggctaa tttgaagctg gaaagcaagc tggcaattat ggagcagtat gtcggtaaaa
    56161 aggtcattga tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga
    56221 ttgtgattca ggaggtactg gaggccagcg atattccgta tcgtcatgac cgccagttgt
    56281 tacatagcgc gctggaaaag gcattacagg ctttaggtta acggaaagcc gaaggaaaat
    56341 tccggcttcc tattgaagac aaagtgcgcg ttgtttatgc cggatgcggc gtgaaagcct
    56401 tatccggcct acaaaccgcg ctaattcaat gtattgcaga gatcatgtag gcctgataag
    56461 cgtagcacat caggcaattt tgcttttgtc atctgtcttc ttcgttatcc tgtcacgaag
    56521 ccgcaatgaa caactcacgc agctgatgca actggtcacg aatttgtgcc gcttcttcga
    56581 actccagatt ctgcgcgtgt tgcatcatca acccttccag ttcatggatt ttctgctgca
    56641 acgctttagg cgacatatcc atcggcacat tatccggctc aacaatcggt cgcgattttc
    56701 ctctgccctt cgctttggtt ttggcaatgt tctgccccag cgccaggata tcgaccactt
    56761 tcttgttcaa gccttgcggc gtaatgccgt gttcctcgtt gtacttctgc tgtttctcgc
    56821 ggcgacgttc ggtttcgcca atcgctttcg ccattgatgg ggtgatctta tcgccgtaga
    56881 gaatcgcttt accgttaacg ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt
    56941 cggaacgcag gaagccttct ttgtcagcgt cgaggatcgc caccagcgaa acttccggca
    57001 tatccagacc ttcgcgcagt aagttgatcc ctaccaatac gtcgaactca cccagacgca
    57061 agtcgcggat aatctccata cgttcgacgg tgtcgatatc tgagtgaaga taacgcacgc
    57121 gctcaccgtg ttcttcgaga tattcagtga gatcttccgc catccgcttg gtcagagttg
    57181 taaccagtac gcgttcgtta attgccgctc gctgacgaat ctccgaaaga agatcatcca
    57241 cctgtgttgc cactggccgc acttcgataa tcgggtcgag taagcctgtt ggacgcacca
    57301 cctgatccac cacatcgcca ccggattttt ccagctcgta attacccggc gtcgccgaaa
    57361 catagatggt ttgcggcgct aatgcttcga actcttcaaa tttcagcgga cggttatcca
    57421 gcgctgatgg caggcggaag ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc
    57481 gatacatgcc gccaatttgt ggaatggtga cgtgagattc atcgaccacc agcagcccat
    57541 cagcaggcag gtaatcaaac agcgtcggcg gtggctcacc cggtccacga ccggagagga
    57601 agcgcgagta gttctcaatc cccgaacagt agcctagctc gttcatcatc tccagatcaa
    57661 actgggtacg ctgggtcagc cgctgctctt ccagcagttt gttgttttcc aatagcactt
    57721 tacgtctggc ggccagctct tctttgatct cctccatcgc ctggacgatg cgctcgcgtg
    57781 gcgtgacgta gtgcgttttc gggtagatgg taaaacgtgg aatagtggaa acaatctgcc
    57841 cggtcagcgg gtcaaataac gacaatcgtt ccacttcctc gtcaaacagc tccacgcgaa
    57901 gtgcaatgtc atccgattct gccgggaaga tatcaatcac ctcgccacga acacggaaag
    57961 taccacgctg gaaggcttga tcattacgag catattgcag ctccgccagt cggcgcagga
    58021 tcgcgcgctg atcgataatc ataccgaccg tgagatggag catcatcttg agatataaat
    58081 caggatcgcc cagaccataa atcgcggaaa cagacgccac cacaaccaca tcacgccgct
    58141 ccagcatcgc tttggtggcg gacaaacgca tctgttcgat atgttcgttt accgaggcat
    58201 ctttctcaat gaaggtgtcg gaactcggta cataggcctc cggctgatag tagtcgtagt
    58261 aggagacgaa atattccacc gcgttttccg ggaagaattc tttcatttcg ccatacagct
    58321 gggccgccag cgttttgttg ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga
    58381 cattggcaat ggtgaaggtt ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca
    58441 ggccatcttc cagcccctct tcgagacgtc gaatcgcctc cggctgatcg ccagaaggtt
    58501 taaaagcgga attcagtttg aacggtttac tcatgagtcg ctacctgaag gagttgggcg
    58561 ggcaggtatg taattttact cgtcgtactt aattatgcca acaaattata ctggataaaa
    58621 aaacagttca tcaccataat atttctgtta cagcgtaaac tccgctgtca atcatgagca
    58681 aaatttactc tgcggcgaga taaaactccg gccttaccgg gttatcccca aagcaacggc
    58741 ttttttaaca tttgtcaaga tgattgatga cagttttatg gcaagagatg cctgttcagt
    58801 gactgccatt gcaattatct aacctgttaa aaaataaaag atatttcttt gagccgtctt
    58861 taacgccaat tcgcgtgcaa gccgcgtatt ctctcgcttg cctcgtgttt tctaactcta
    58921 atacacatgg ttatccacag gaatagtgga taactgtctg cagcccctat cccccgccgc
    58981 ctgggcaatt cccacattca ccgcatgatg cggaggtaaa atttttttcg tgattttttt
    59041 agaatttaat tggttaaatt gcagtcaatc ggagacacga tctcgctcgc aatttaacca
    59101 attacaggat gactacatta aggcaagatt gatgtacttt ccggttgccg cattttctgg
    59161 attttctgca agccagggaa tttcgcccag cagcggcgcg ggaatcatgc gggtgagcgt
    59221 ggtcatatat tcagcgtgac gttttcccgg aggcgtgaca tcgttcgcca cccaccccgc
    59281 cagagtcagt ccggcgtgta gtattgcctg cgcagtcaac atcgcgtgat taatacagcc
    59341 gagtttcaca ccaactacca gtatcaccgg cagttgttcc tgtgttaccc aatccgcaaa
    59401 agtgaaagtg tcagaaagcg gcgtaaacca gccgccagca ccttccacta acacccagtc
    59461 agcctgttgt tcaagcgcgc gtaatccggc gctcattacc aatgattcta tcggcctgcc
    59521 ctcttgcgcg ctgatgatat gcggcgaggt gggttctgcg aaggtgtaag gatttaccgt
    59581 tgcgtaatcc agctgcaggc tactgttgcg ctgtaacgcc agcgcgtcac tattacgtaa
    59641 gcctttcggg gtcttttcgc tgccagaggc caccggttta taacctgccg tccggtagcc
    59701 tgctgccttt gcggcttgta aaagcgcgca gctggcgaca gttttcccca cttcggtatc
    59761 cgttccggtg acaaaataac atttactcac gagcaatcac tcccaaaaaa agatgatacg
    59821 tcagaggata tcgcccctgt tgttgcggcc aggctagttg caatcgctgc aactgcgaac
    59881 gcgttaatat tcgcgggtcg cgcccttcat gaagatgcgt ggcaccgatg cctttcagcg
    59941 aacgcatggc actgagcgca tcatcaaacc acagcgtaat gggctgaata taatgttgat
    60001 aatgcacggc gtttagcgac tgttcgattt tatctggcgg taaaaagcga ttagcatgcg
    60061 ggcgctcgtc caccgcctgc cacgcctgat gcagttcggg taacgatccc tgcaccagcg
    60121 tggtaaacgc gaccacgcct ccggggcgca ccacccgata cagctcgcgg agtgccatgg
    60181 ataaattacc gcaccactgc accgcgagat tgctccatgc aagatcgaac gttgcagtcg
    60241 ctaacggcag ggattcgata tctcccgcca gataatggtc tgcggcatcc ttctggcgtg
    60301 cctgaacaag cattggcgga gagagatcta acgcagtcac ctgcgcgtga cgctctcgcc
    60361 agcggcggct catccagcct ggtccacaac ccgcatccag tacgtgggtg tatttacgct
    60421 gtgggagcat tgccagtaag gcgtcagcac tctggcgctg tagatcggcg tgttgctcat
    60481 agtgtgcggc tgcccgtcca aatgccgctg caatggcttg tttattaacc gttgccatgc
    60541 agcacctcca gcagacggtc gatatcctgc atttcatgcg cggctgttag tgttaagcgc
    60601 agtcgcgcag taccagcagg tacggttggc gggcgaatcg ccgtgaccca gcagccttgc
    60661 tggcgcagtt tttctgccag ttgtaacgca cggctgttat caccaacaat caatggctgg
    60721 atggcgctgc atgaatcagc aagcgtaaac ggcaaatcct gtactccggc acgaaaacgc
    60781 gtaatgagtg ccgccagttt ttcgcgccgt gtatcaccct catcactgcg aatgaccgcc
    60841 agcgatgcac gtaatgcctg cgcctgagcg ggcggcatac tggtgctgta aataagatgg
    60901 cgggcaaatt gcaccagata atccgccacc gtatcggagc aaagcaccgc tgccccgctg
    60961 acgccaaatc ctttgccaaa agtcaccacc agcaattctg gttttacctt ttgcagccag
    61021 cagctgccgc gcccctgctc cccgataacg cccgtgccgt gggcgtcatc gaccatcaac
    61081 cagccatcgt gctgttgcgt tacctgctgg atttcctcca gtggcgcact atcgccgtcc
    61141 atgctgaaca caccttctgt cactaccagt tgctgccccg gacaggggga agcaagcagt
    61201 cgcgccagat gagtgacatc gttatgagca aaacggcgaa gcggcgacgg gcttaaactt
    61261 gcagcctcca gcaatgaggc atggctaagc cggtcggcaa caatacggtc ctctttcgcc
    61321 atcatcgcgg caatgactgc ctgattagcc gcaaaaccgg agataaacag cagcgcccgc
    61381 gaatagccca gccactcggc cagttcttct tccagcgcct gatgtgccac gctataaccg
    61441 ctgacgtgac cggagccgcc gctaccgacg ccaaattgct ccgcactctg cttccaggca
    61501 cggataattt gcggatgatg gcttaaacct aaataatcgt tactggaaaa gttcagatac
    61561 tggcgatcgt ccgccaccag ccagcgtccg gccccttgcg ccaccggata acggcgacgc
    61621 agggcatcgg cagcacgccg cgcatcgagc gccgcgttga ttttctcctg ccagctcata
    61681 gtgctgccgc gttgtaatat tcgtcagtgt ccggggtcat cagtgcctgc tccagacgct
    61741 gctgttgttc gttatcccct gccagcacgg cagtttgctg tggatttagc cccagtttgc
    61801 ggaacagttg caggtcttta tcttcttccg gattcggcgt ggttagcagt ttgcaaccgt
    61861 agaaaatcga gttagccccg gccataaagc acatcgcctg agtctgttca ttcatctgct
    61921 cgcgtccggc agaaaggcgc acgtatgagg tcggcatcat gatccgcgct acggcaatgg
    61981 tgcgaataaa atcaaaggca tcgacatcat cgttatctgc cagtggagta cctttcacct
    62041 tcacgagcat gttgattggc acgctttccg gcggcgtcgg caggtttgcc agttgcagta
    62101 ataatccggc acgatctttt accgtttcgc ctaaacccac aatgccacct gagcagactt
    62161 tgatcccggc atcgcgcact ttttccagcg tatcaaggcg ttcctgataa gtacgtgtgg
    62221 tgatgatatt gccgtaaaac tccggcgagg tgtcgaggtt atggttgtag taatccagcc
    62281 cggcattcgc aaggcgctgc gcctgagatt cactcaacgt acccagcgtc atacacgcct
    62341 ccagccccat cgcttttact ccctgcacca tttgttccag gtagggcata tcgcgttcgt
    62401 ggggattctt ccacgccgcg cccatgcaga agcgcgtcga tcctgccgct ttcgctttgc
    62461 gcgccgacgc cagcacctgt tcaacttcca tcaaccgctc ggcttccagc ccggttttgt
    62521 agcgcgagct ttgcgggcag tatttgcaat cttccggaca agctccggtc ttaattgaca
    62581 gcaaagtgct gacctgcacc tgacgtggat cgaaatgctg acgatgcacc tgctgcgctt
    62641 caaacagcag atccagcaac ggtttttcaa ataattctgt gacttgcgac aatgtccagc
    62701 gtgggcggtg agccatgggg cttctccaaa acgtgttttt tgttgttaat tcggtgtaga
    62761 ctcgtaaacc taaatctttt caatttggtt tacaagtcga ttatgacaac ggacgatctt
    62821 gcctttgacc aacgccatat ctggcaccca tacacatcca tgacctcccc gctaccggtt
    62881 tatccggtgg cgagcgccga aggttgcgag ctaattttgt ctgacggcag acgcctggtt
    62941 gacggtatgt cgtcctggtg ggcggcgatc cacggctaca atcacccgca gcttaatgcg
    63001 gcgatgaaga cgcaaattga tgccatgtcg catgtgatgt ttggtggtat cactcatgcg
    63061 ccagccattg agctgtgccg caaactggtg gcgatgacgc cgcaaccgct ggaatgcgtt
    63121 tttctcgcgg actccggttc cgtagcggtg gaagtggcga tgaaaatggc gttgcagtac
    63181 tggcaggcca aaggcgaagc gcgccagcgt tttctgacct tccgcaatgg ttatcatggc
    63241 gatacctttg gcgcgatgtc tgtgtgcgat ccggacaact caatgcacag cctgtggaaa
    63301 ggctatctgc cagaaaactt gtttgctccc gccccgcaaa gccgcatgga tggtgaatgg
    63361 gatgaacgcg atatggtggg ctttgcccgc ctgatggcgg cacatcgtca tgaaatcgcc
    63421 gccgtgatca ttgaaccgat tgtccagggc gcaggcggga tgcgcatcta ccatccggaa
    63481 tggttaaaac gaatccgcaa aatgtgcgat cgcgaaggta tcttgctgat tgctgacgag
    63541 atcgccaccg gatttggtcg taccggcaaa ctgtttgcct gtgaacatgc agatatcgcg
    63601 ccggatattt tgtgtctcgg taaagcctta accggcggca caatgaccct ttccgccacg
    63661 ctcaccacgc gcgaggttgc ggaaaccatc agtaacggcg aagccggctg ctttatgcat
    63721 gggccaactt ttatgggcaa tccgctggcc tgcgcagcag caaacgccag cctggcgatt
    63781 ctcgaatctg gcgactggca gcatcaggtg gcggctattg aagggcagct acgcgagcaa
    63841 cttgcccccg cctgtgacgc cgaaatggtt gccgatgtgc gcgtactggg ggcaatcggc
    63901 gtggtcgaaa ccactcgtcc ggtgaatatg gcagcgctgc aaaaattctt tgtcgaacag
    63961 ggtgtctgga tccggccttt tggcaaactg atttacctga tgccgcccta tattattctc
    64021 ccgcaacagt tgcagcgtct gaccgtagcg gttaaccgtg cggtgcagga tgaaacattt
    64081 ttttgccaat aaccgggagt cagcgtgagg ggttctggct acactttctg caaactagaa
    64141 aggagggttc atgaaactca tcagtaatga tctgcgcgat ggcgataagt tgccacatcg
    64201 tcatgtcttt aacggcatgg gttacgatgg cgataatatt tcaccgcatc tggcgtggga
    64261 tgatgttcct gcgggaacga aaagttttgt tgtcacctgc tacgacccgg atgcgccaac
    64321 cggctccggc tggtggcact gggtagttgt taacttaccc gctgataccc gcgtattacc
    64381 gcaagggttt ggctctggtc tggtagcaat gccagacggc gttttgcaga cgcgtaccga
    64441 ctttggtaaa accgggtacg atggtgcagc gccgccgaaa ggcgaaaccc atcgctacat
    64501 ttttaccgtt catgcgctgg atgtagaacg tattgatgtc gatgaaggtg ccagcggcgc
    64561 gatggtcggg tttaacatcc atttccactc tctggcaagc gcatcgatta ccgcgatgtt
    64621 cagttaatta ctctgccaga tggcgcaaag ccatctggta tcacttaaag gtattaaaaa
    64681 caactttttg tctttttacc ttcccgtttc gctaaagtta gtataaaaaa gcaggcctca
    64741 acggattcat ttttctattt catagcccgg agcaacctgt gaacacattt tcagtttccc
    64801 gtctggcgct ggcattggct tttggcgtga cgctgaccgc ctgtagctca acaccgcccg
    64861 atcaacgtcc ttctgatcaa accgcgcctg gtacctcttc tcgcccgatt ctgtcggcaa
    64921 aagaagcgca gaatttcgat gctcaacact attttgcatc cctgacacca ggtgcggcag
    64981 cgtggaatcc ttccccgatt accctgcctg cacaacctga ctttgttgtc ggcccggcgg
    65041 gtactcaagg tgtaacgcat accacgattc aggcggcggt agatgcggca attatcaagc
    65101 gtaccaacaa gcgccagtat attgccgtga tgcctggtga gtatcaggga acggtatatg
    65161 tccctgccgc tccgggtgga attactctgt acggtacagg tgaaaaaccg attgatgtga
    65221 agattgggct ttcccttgat gggggcatga gccccgccga ctggcgtcac gacgtcaacc
    65281 cgcgcggcaa atatatgcca ggtaaaccag cgtggtatat gtacgatagc tgccagagca
    65341 aacgcagcga cagtatcggc gttctctgct ctgcggtctt ctggtcacaa aacaatggac
    65401 tgcaactgca aaacctgacc atcgaaaaca cgctgggcga tagcgtagat gcgggtaacc
    65461 atccggcggt ggcactgcgt actgatggtg accaggtaca gattaacaac gttaacattc
    65521 tcggtcgtca gaacaccttc tttgtcacca acagcggtgt gcagaaccgt ctggaaacga
    65581 atcgtcagcc gcgtacgctg gtgaccaaca gctacattga aggggatgtg gatatcgttt
    65641 ctggtcgcgg cgcagtggtg ttcgataata ccgaattccg cgtggtgaac tcacgtactc
    65701 agcaagaagc gtatgtgttt gcaccggcta cgctgtccaa catttactac ggtttcctcg
    65761 ccgtaaacag tcgtttcaat gcttccggtg atggcgtggc gcaactgggc cgctcgctgg
    65821 atgttgatgc caataccaac ggtcaggtgg tgatccgtga tagcgccatc aacgaaggtt
    65881 ttaacacagc caaaccgtgg gctgatgcgg tgatttccaa tcgtccattc gcgggtaaca
    65941 ccggcagcgt tgatgataac gacgaagtac agcgcaatct gaatgacact aactacaacc
    66001 gcatgtggga atacaataac cgcggcgtgg gtagcaaagt ggttgcagag gcgaagaagt
    66061 aggagctgtt aactatgtgc cggatgcggc gtaaacgcct tatccggcct acggttcgat
    66121 gcgatttgta ggtcggataa gatgcgccag catcgcatcc gacaataagt gccggatgcg
    66181 acctacattc acatggcgct ttttacatct gacggttttt attgaagtta atcaaactac
    66241 ccgccttgat aatctcgcgc tcttcagcag tcagactttc catatagagc gtaatctccg
    66301 ttaccggtgc atcttcatgg atcacataac ctttaaacgt cgttccagga ttatccagcg
    66361 ccgctttaat gccagggatg taaatgtaat cccccacttc aaacgttggt gcttccgcca
    66421 tttgcagcgg taacatcccc cagttgatga cgttagaacg gtagcgttta gtcgcgtatt
    66481 cctcggcaat attcgccaga ccgccaatca cacgctggca gctcgccgcc tgctcacgcg
    66541 cagaaccatc gcctggtttc accgcataga ccatgctgcc aatttcagtt tgcagcggat
    66601 caatatgctc ctgaccagca atctgcttaa tgcgcgcaaa cacctctgtc agctcgctga
    66661 cattccccgc cagacgctga ttttccagct cagcagtcgc tttacttctg ccaacataac
    66721 cgggatcgcg gcgtgacagg gtaaactccg ccagaccaat cggatttgaa cgataagaag
    66781 aggtttcacc ggaaggaatc agttcgtcgg tggtggtgac ttcgtcgagg atcttcgagc
    66841 acactttgag gacgatattg tcagtcagcg cgcccaattc cggccagtct ttgatgttcg
    66901 gtccgtagat cagcggttgc tgggtcgccc ctttcacaaa gccttgatag acgcgatttt
    66961 tatacggcgt tacatcgaag gcgtactccg gcacgttgtc ccagcaatcg agttcgctgg
    67021 cagaggttaa atagccaccg tttgccgcag ttgcagcgat agaacgagcg tccatcaacg
    67081 ccaccgctga catctgccca ttagctggct tagagccttc gcggttcggg aagttacgcg
    67141 tggtgtggcg aatactcaaa ccgttgttga ttggcgtatc gcccgcacca aagcatgggc
    67201 cgcagaacgc ggttctgatg attgcgcctg cgccaatcaa atctgctacc acaccttttt
    67261 tggcgagatc cataaacacc ggctgtgatg acgggtaaac cgccaatgag aaggtatcgt
    67321 tgccgcagga ttgaccgcgc agcgcattcg ccgccgcgat gacgttttcg tagttaccgc
    67381 cagaacagcc cgcgataatc ccctgctgca ctttcaggcg accattttcc actttatcca
    67441 gcagcgagag tttggcttta ccgtgcgcca cgcgttcgga ctcaatttca atctcacgca
    67501 gaatgtcggt caggttctgg ttcagcgtgt cgatttcata cacgttgctc gggtggaatg
    67561 gcagcgcaat cattggtttg atggcgctta aatcaacgct gatgcagcca tcgtagtacg
    67621 ccattggttg agggttaagt tggcagtaat cctggccgcg accgtgcagc gccagccagt
    67681 tatggacttc ttcatcggtt tgccagacag aacttaaaca ggtcgtttca gtggtcatca
    67741 cgtcaacgct gttacggaaa tcggtagaga gcgctgcaac accgggacct acgaactcca
    67801 tcactttgtt tttgacgtaa ccgtttttga acacggcacc gatgatagcc agcgccacat
    67861 cctgcggccc cacatacggc gcaggttttc cggtcagatg caccgcaacc acgcccggat
    67921 agtcgatatc ccaggtgtca ttaagcagct gttttaccaa ctcgccgccg ccctcgccaa
    67981 ccgccattgt ccctaacgca ccgtaacggg tatggctgtc tgacccgagg atcattttgc
    68041 cgccgcccgc catcatttca cgcatatatt gatggatgac cgcaatatgc ggtggcacaa
    68101 aaataccgcc ataacgctgg gccgccgata aaccaaaaac atggtcatca ccgttaatgg
    68161 tgccgcccac agcacagagt gagttatggc agttggtcag cacatacggc agcgggaaac
    68221 gttccatacc ggacgcttta gcggtctgta caataccaac aaaggtaata tcgtgagagg
    68281 ctaatgaatc aaacttaatt ttaagtttat ccatatttcc ggacgtatta tgagaagaga
    68341 gaatagacca ggcaatagtg cctttttttg cttcttcttt ttttatttcg ccggtgaaat
    68401 gttcttcggc aattatttcg ttattgctgg cgagaaacac gcctttttca gataacttga
    68461 tcatcaaatg ctccagatat ctgcgcaccg tgaagtgcgc agaaagataa taaatggata
    68521 tactctaaat aattcgagtt gcaggaaggc gacaagcgag tgaatcccca ggagcttaca
    68581 taagtaagtg actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgacga
    68641 gtatatttac agcatgttcc agccgatcag catattccac caccacacgc cgagggtgat
    68701 atgcaccagg aaggttaata tcgtcagtac cgcaccgacc aaccaccagg atttaatatc
    68761 gttgtaaccc acgccaaaga tgaccggacc tgccgcgccg ccatagtgag taaccatgcc
    68821 gccataggag ttagagaaca acagtgccag cgcggttaac attaacggtg cgccggagac
    68881 gttcgccagc atggcaaata caggtaacat agcaacgata taggcactac cggaagcgaa
    68941 gaaataacgc acgatgatgc tgaggaaaat aataacgaag aaagcaacgt taccgtgacc
    69001 atcaaatgcc aggttatttt taaagacttc agctaaccat tcaaagaatt taactttcga
    69061 taataaggag cttaagccga taataccgcc gtaccagatt aaggtattcc agccgccttt
    69121 atttttaacc acgtcttccc aggtaacgat acccagcagc agcatagtcg ccataacaac
    69181 gattgccacg gtggattcat caacccccag tgacttactg aaaatccagc ccagcagcgc
    69241 cagcacgaag acaccgagca gcattttttc gcggattttc atcggtccca gttcggcaag
    69301 gcccgctttg gcgatggttt tgttatccac cttcttaatt tctggcggat acatggtgta
    69361 aatcaccagc ggggtgacca gcagcataat gatacccggc aatccagcgg ccagcgccca
    69421 tccaccccag ctaatttgca ggtgcagaat gtcgttgatc attttcagcg ccagaatgtt
    69481 ccccgccatt gcggtaaaga acatatagct ggtggtttta gtgaccatgt aaatggacat
    69541 catcaggtaa tgtccaacac gacgcggact tttttccggt tctgatccca aagccactgc
    69601 cacgctgttg atgatcggta acacgatgcc gcccgcacgc gcggtgttag acggtgttgc
    69661 cggagccagt accagatcga gaaataccgt aacgtaaccc agacccaggg tagtgctacc
    69721 aattttacca atcagcagat aggcaatacg tttacctaag cctgtggtta caaacgcggc
    69781 gcttaaagta aacgcagaaa acaccagcca ggtggtaccg gaagagtaac cgcttaatac
    69841 ggcggtggtt ttaaattccc cacctgataa gttacccacc actaccatcg atgcggcgac
    69901 ggcaattaac agtacgacag gttccgggaa aggcttgata accagcccca caatggccgc
    69961 caggtaaata ccaaaaagca cccacgccag ttcgcttaat cctgccggag ctggcataaa
    70021 gccaataata catgggatcg ctaatatcag aattagcttc cataacgatt ttttattcat
    70081 ttaatatcct gttctttctg tatgactcat acagaattcc tttattgatt gtcattatca
    70141 cgacaacaaa atttttcagg gaagataaac ttcaccggaa aatatttttc ttgtcgtccg
    70201 aataacagat gcgcgtaacg tcgtggcatc ctgaccttca ttacttaaat gaacgtcgag
    70261 cgcaccactg gggtgttcaa tattgatatt gccgtatcct acagaaggga cgatttgtcg
    70321 ggtgacggtg ccttccaatg cacaactaca ggaaatagca atagcaccgg ttatcgccaa
    70381 agcgcgatgg caagaatgcg gcataaaata acgcacatta attgccccgc ctttctgcgc
    70441 tggagaaata agtacaggtt tagggataac catattactg acatcgccta agcccattgc
    70501 tttacccgct tgtagacgga tagattcaat gctggctaat aatgctttgt cggcatccag
    70561 ctctgccggt aattcatagc ctgtttttcc cagatattca gcaggaataa tgacaactgg
    70621 catcgccata tcgatacagg tcaccgggac atcgtcaaaa taatcaatct gattatccgt
    70681 cgggaagact tttccggttt tcgttccggc ggcattcagg aaagtgagcg caaccggtgc
    70741 ggcagtaccc ggtacgccgt caattctggc gctaccctcg tactcgacaa caccatttgg
    70801 cgtttgcaca tcagcttcga taaacgtacc cgtattgacg ttgcggatac gtacgcgggt
    70861 aactggcgaa gtcgctgcaa tcaaaccatt ttcaatggcg aatgccccaa cgccagacag
    70921 catattgccg cagttaggcg tggtatcgac acgttgctca tggacgataa cttgagcaaa
    70981 cagataatcg acatctgcac gcggatcgct ggagcggcta ataatggcga ctttactggt
    71041 cagcggatta ccgccgccaa taccgtcaat ttccagatcg ttaccggaac ccataattgc
    71101 catcaatatt ttatcgcgct gcgtttgatc ttcgggtaga tgttccgcta acaggaacgc
    71161 gcccctcgag gttccacctc gcatcatcac gcagggtatt tttttcataa taatgccctc
    71221 aatttaaaaa cataacttat tgcgatatgg ttacattaag ggcaaagcat ctctaatgac
    71281 tttagctcaa cgattaataa cattttttca ttaatttaag ttggctaaat gcagttaaaa
    71341 tttttaactg ccagtcaccc aaaatcgtcg cttccatttt ccggagtaat aatgaagcat
    71401 gaattatcaa gtatgaaggc atttgtcata ctggcagagt ccagttcatt taacaatgct
    71461 gctaaattac tcaatattac gcaacctgca ttaacgcgaa gaataaaaaa gatggaggaa
    71521 gatttacaca ttcaactatt tgagcgtaca actcgcaagg ttacgttaac aaaagcagga
    71581 aaaaggttgc tcccggaggc gcgggaatta ataaaaaaat tcgatgaaac gctttttaat
    71641 attcgtgata tgaatgctta tcatcgtggt atggtgacgt tagcgtgtat tccgaccgca
    71701 gtattttatt ttttaccgct ggcaatcggt aaatttaatg agctgtatcc caatattaaa
    71761 gtgcggattc tggaacaagg cacgaataat tgcatggaat cggtgctgtg taacgaatca
    71821 gactttggca taaacatgaa caacgtcaca aattcatcca ttgattttac cccactggtc
    71881 aacgagccgt tcgtgctggc ctgtcggcgt gaccatccat tggccaaaaa gcagcttgta
    71941 gaatggcagg agttagtggg ttacaaaatg attggcgtac gctcctccag cggcaaccga
    72001 ctattgatag aacaacagct agccgacaag ccctggaagc tggactggtt ttacgaagtg
    72061 cgtcatcttt cgacgtcatt agggctggtg gaggcgggac tggggatttc ggcgcttcct
    72121 ggtctggcaa tgccccatgc gccctattct tcgattattg gtatcccgct ggtggaaccg
    72181 gttatacgtc ggacattggg gattattcgc cgcaaagatg ccgtgctttc tccggcagct
    72241 gaacgctttt ttgccttgct gattaatttg tggactgacg ataaagacaa tttgtggacc
    72301 aatatcgtcg aacgccagag acatgcgtta caggagatag gttaatacac cggcgcagaa
    72361 gagactgcgc cgggtaaatc agcggttagt gtgcgttaac caccacccac attggtccct
    72421 gcccgaccgc atagcggcct ttttcatgca gtagcccctg ctcgccaaca atttcgtata
    72481 ccgagatgtg gtgagatttt tgcccggcgg caatcagata cttgccgctg tgatcaacat
    72541 tgaagccgcg cggctgggtt tcagttggct ggaagccttc tttactcaac acgctgccat
    72601 cttccgaaac gctgaaaaca gtaatcagac tggcggtacg gtcgcaggcg tataaatgac
    72661 gaccatccgg ggtgatatga atatcagccg cccaacgggt gtcggagaag ttttccggca
    72721 tcatatccag cgtctggaca cattcgatat tgccatgcgg atctttcagt tcccagacat
    72781 ccactgagct gtttaactca ttgacgcaat acgcatattg ttcgtttgga tggaatacca
    72841 tatgacgcgg gccggcccct tcaacggtag tcacttccgc agggtcctgc gccaccagat
    72901 gaccatcctc gctgacagta aacaggcaaa tgcgatcctg ctttaatgcc ggaacccaca
    72961 gcgtacggtt gtccggtgag atattggcgg aatggcaacc gtccagcccc tcgaccacat
    73021 cgacgacgcc cactggcagg ccatcttcca gacgcgttac gctcacgtta cccgcattgt
    73081 aagaaccgac aaagacaaac tgcccctggt gatcggtgga aatatgcgtc ggactacccg
    73141 gcagcgcaga ctctgcggca aaggtcagcg cgccatcgtc cggggcgata cgatacgcca
    73201 ggacgcgaaa ctcagggcga acaccaacat agagataacg tttgtccggg ctgaccacca
    73261 tcggctgcac ctgccccggc acgtcgacaa cctgtgtcag cgtcagtgcg ccttcatgat
    73321 tcaggttcca gacgtgaatt tgctggctct cagggctggc gatataaact gtttgcttca
    73381 tgaatgctcc tttgcattta gcaactgact gtaacagcta aaattagtcg cttttggcgg
    73441 tgaatgctga attaaaacgc agaattttgt ccgatagtgg tatcggtgta ccattcgcgc
    73501 agacgaaaat tcttaacctt aatctggaat acgccatgac cacacgcgtg attgctctcg
    73561 acttagacgg caccttattg accccgaaaa agaccttgct tccatcatcg atagaagccc
    73621 tggcccgcgc tcgcgaagca ggctatcaat taatcatcgt cacaggtcgc catcacgtcg
    73681 ctattcatcc tttttatcag gcgctggcgc tggatacacc tgctatttgc tgtaatggca
    73741 cctatttgta tgattatcat gcaaaaacag tgctggaagc ggacccaatg cccgttaata
    73801 aagccctgca actcattgag atgctgaatg aacaccacat tcacggtctg atgtatgtcg
    73861 atgatgcaat ggtctatgag cacccgaccg gacatgtcat tcgcacgtct aactgggcgc
    73921 aaaccctgcc gccggaacag cgtccgactt tcacacaagt cgcttctctg gctgaaacgg
    73981 cgcaacaagt taacgccgta tggaagttcg ccctgacgca cgatgacctg ccgcaattgc
    74041 agcattttgg taagcatgtc gaacatgaac tgggactgga gtgtgaatgg tcctggcacg
    74101 atcaggttga tattgcacgc ggcggcaaca gcaaaggtaa acgtttgacg aaatgggttg
    74161 aggcgcaagg ctggtcgatg gaaaacgtcg tggccttcgg cgataacttc aatgatatta
    74221 gtatgctgga agccgctggt acaggcgtgg cgatgggcaa cgccgatgac gcagtaaaag
    74281 cgcgcgccaa cattgtgatt ggtgataaca ccaccgacag cattgcccag ttcatttata
    74341 gccacctgat ttaatcaggc ggttatcgac acacttttaa tttgcgcata cagccacagg
    74401 ccaggtttga tcgccagttc atccctggcc cacgggctga tacgcgccca cagcgtttta
    74461 ccgccgactt ccagctccac ttccacctgg ccgttgtcgt cataactatt aaccactttt
    74521 gcccgcaata cgttacgaat gctggtttgc tgcggcggtt gtaaaaccag cgagacatcg
    74581 gaagcctgaa tgcggatgcg tagcgcagct tgcagcggtt cgtccagctt attgacccac
    74641 aaatgctgat cgcccagcgc cagcgcggtc atcgcgtaat gcggatgatg ctcaagcacc
    74701 gtcactttca gaatgctact ttgttgctct ttcggcagcc acggattcat cacgctactg
    74761 ccccacactt cctccagcgc gccaaaggct ttcacctgac cgttttccag taccatcact
    74821 ctgtctgcca gatggaggat ctcatccagc gaatggctga catacaacat cggaatgttg
    74881 atttcccgtg tcagccgttg cagataaggc aacagttcgc gtttacgcgg aatatccagt
    74941 gacgccagcg gttcatccag caacagcaat tccggtgctg tcagcaaagc ccgaccaatc
    75001 gccacgcgct gtttttcacc tccggacagg ctgcctggta aacggtcaag caacggttca
    75061 atgcctaaaa gcgccaccag cttatcgaac tgatcgacca tacttttcgc catgccgtag
    75121 cgcagattgc cacgcacttt gtaatgcggg aacagccgcg catcctgaaa aacatagcca
    75181 acgcgacgct tttccggcgt caggcagata cctttttcgg catcatttag tacccgccca
    75241 ttgaggacaa tccgcccttt ttgcgggcgc gtcagtccac tgatggcgtt aatcagcgaa
    75301 gtttttccgg caccggagac gccaaagata gcagtgatgc cattggcggg cagcgtttca
    75361 ttaatggtca ggcaatggtt gcccaacgtc tgggaaaaat tcagttccag catgattagc
    75421 gccccgcccg ttcacggctg attctggcca gccattctga aatcaacagg gagatcatcg
    75481 ccagtgcaat agaaataatg cacagtctcg ccgctccact ttccccgccg ggggtctgga
    75541 tcagggtata catggcagaa ggaatggttc gcgtttcacc aggaatgttc gacacaaagg
    75601 tgatggttgc accaaactca ccgagagaac gagcaaaagc cagtaccgta ccaacaataa
    75661 tgcccggtaa ggtcagcggt aacgtgatag taaagaaaac gcgccagcgc ccggcaccca
    75721 gtgttcttgc ggcctgttcc agtttgacat caaccccttc cagcgccaga cgaattgccc
    75781 gcaccatcag cggaaacgac atgacggcgg cagcgagaac cgcgccgcgc cagctaaagg
    75841 cgaaggtaat gccaaaccag tcatacagac gttcaccgat aaatccgcgc cgtcccatcg
    75901 aaactaataa taagtaaccg acgaccacgg gcggtaacac cagcggtaga tgcagtacgc
    75961 tgtcgagcag agctttgccc ggaaacgtgc aacgcaccag taaccaggca aaaaagatcc
    76021 caaacggcag gctaaacagc acagccaggg aagaaacttt caggcttagt aaaaccgcct
    76081 gccattctgg atcggtcagt atcattactt ggttgtaaat ccgtaacgtt taaagatttc
    76141 cgcagcctgc ggtcccttca gataatcata aaatgctttc accgtcgcat tgttatgccc
    76201 ttccacaaca gcaaccggat attccacttt tttatgcgaa tcttccggga aggtggcaac
    76261 cacttttacc cctttgctgg caactgcgtc agaaccgtaa acaataccca gcggcgcttc
    76321 attgcgttcg accaacgcca gcgcgccacg aacatcttcc gccggagcca gtttcgggga
    76381 gagcgtatcc catgcgccca gtttttgcag tgcttctttt gcataaatgc cagcgggaac
    76441 atgttccgga tcgccaaccg ccagacgacc gccattcagc agcgaagtcc agttggtttt
    76501 gctgtcgatg gtgaaatctt tctgctcgct ggctttcggt gctacaacga ccaggctatt
    76561 gccgagcagt gtctggcgcg aagccgtatc gatcgctttt ttatcaaccg cataatccat
    76621 ccatttctga tcggcagaaa taaacagatc cgcaggcgca cccgcttcaa tctgacgggc
    76681 caaagtagat gacgaagcga aagaagaaac cacatccacg cctttctctt ttttatactg
    76741 cgtagcaatg tcctgcattg cgttagtcag tgatgcggcg gcgaacaccg tgattttccc
    76801 ttcatctgcc agtgcattgc cagcaacagc gaaagagagt gccgccccgg caaacaagtt
    76861 caaccattta cgagccattg gtaacccctt aatctaacgt tatgtaggcg acaatataac
    76921 ggctaactca gaattttccc agcagttatt gctaaccttt gtgaggtaga tgagaaaaaa
    76981 tatcggcagg aaaagcagga ggttgagaaa aagaaatgcc cgactaagcg ggcattcagg
    77041 gattcaatga ttttgtccgg gctggtcttt tttaccaaca ccagaaaaga tgttgaatac
    77101 ttcaccaaga ccgtaaatca gacccaggat gatggccatc acgacaggta ccatgattac
    77161 ggcgaatacc agacttttta ataactctaa catggtcaac tccagatata gtcatgagac
    77221 tattctaacc gctaagcaaa gaaaagcact ccccttttgt gcggtcagct ttgcgtggcg
    77281 ttcgttttcc gtcacaataa gactttttcc aggatattgt tatgcaggcc gaaatccttc
    77341 tcacccttaa gctccaacaa aaattattcg ccgacccgcg ccgcatttcg ctactaaaac
    77401 acattgcgct ttccggttcc attagccagg gagcgaaaga tgccggtatc agctataaaa
    77461 gcgcatggga tgccattaac gagatgaatc agttaagtga gcatattctg gttgagcgcg
    77521 caacaggcgg taaaggtgga ggcggcgcag tactgacccg ctatggtcag cgactgattc
    77581 agctctatga cttactggcg caaatccagc aaaaagcctt cgatgtgtta agtgacgatg
    77641 acgccctgcc gctgaacagc ctgctggccg cgatctcacg tttttcactg caaaccagcg
    77701 cccgtaacca atggtttggc accatcaccg cccgcaatca tgatgacgtt caacagcatg
    77761 ttgatgtctt actggctgac ggaaaaacac gcctgaaagt cgcaattacc gcacaaagcg
    77821 gcgcgcgtct ggggctggat gaaggcaaag aagtgttgat attactaaaa gcgccgtggg
    77881 taggcattac tcaggacgaa gcggtcgcac aaaacgccga caaccaatta ccgggtatta
    77941 ttagccatat tgagcgcggc gcagagcagt gcgaagtatt aatggcgcta cccgacgggc
    78001 aaacactgtg cgccacagtg ccggtaaatg aagcgacttc tcttcagcaa ggacagaatg
    78061 tcacggccta ctttaatgcc gacagcgtga ttatcgccac gctgtgctaa gcgtgttgac
    78121 aatttgttat gaaacacgta tccctgtcag taatcgctgc acaaaatggg atataaaatg
    78181 tcatcgttgc aaattttgca aggcacgttt cgtcttagcg acacaaaaac gctgcaattg
    78241 cctcagctaa cgttaaacgc gggtgatagt tgggcgtttg tcggttcgaa tggaagcggg
    78301 aaatcggcgc tggcccgcgc gcaggcgggg gaacttccgc ttttgaaagg tgaacggcaa
    78361 agccagtttt cccacatcac tcgtctctcc ttcgagcaat tgcaaaagct cgtcagcgac
    78421 gaatggcagc ggaataacac cgatatgctc ggccctggcg aagatgacac cgggcgtacc
    78481 acggctgaaa tcattcagga tgaagtaaag gatgcaccgc gttgcatgca actggcgcag
    78541 cagttcggta ttaccgccct cctcgaccga cgctttaaat acctttccac tggcgagacg
    78601 cgaaaaaccc tgctgtgtca ggcgctgatg tcggagcctg acttgttgat tcttgatgag
    78661 ccgttcgatg gcctggatgt tgcctcacgt cagcagctgg ctgagctact cgcctcgtta
    78721 catcagtccg gtattactct ggtactggtg ctcaatcgct tcgatgagat cccggaattt
    78781 gtccagtttg ctggcgtgct ggcggattgt acgttagcgg aaacgggcgc taaagaggaa
    78841 ctgctccagc aagcactcgt cgcgcaactg gcacatagcg aacagcttga aggtgtgcaa
    78901 ctgccggagc cggatgaacc ttcagcacgt cacgccttac ccgccaacga accgcgcatt
    78961 gtgctgaaca acggcgtggt ttcttataac gatcgcccca ttcttaataa ccttagctgg
    79021 caggtgaatc caggcgagca ctggcaaatt gtcgggccaa atggcgcggg aaaatcgacg
    79081 ttattaagcc tgattactgg cgatcatccg caaggttaca gcaacgattt gacgcttttc
    79141 ggacgacgtc gcggcagcgg cgaaaccatc tgggatatca aaaagcatat cggttacgtc
    79201 agcagtagtt tgcatctgga ttaccgggtc agcactaccg tgcgtaatgt gatcctttct
    79261 ggctattttg attcgattgg catttatcag gccgtttcgg atcgccagca aaaactggtg
    79321 cagcagtggc tggatattct cggcattgat aaacgcacgg ctgacgctcc gttccatagt
    79381 ctttcctggg gacagcagcg tctggcgctg atcgtccgcg cgctggtgaa acatccgacg
    79441 ttgcttattc tcgatgaacc actacagggg cttgatccgc tcaatcgcca gcttatccgc
    79501 cggtttgttg atgtgctgat tagcgaaggt gaaacgcaat tgttgtttgt ttcgcaccat
    79561 gctgaagatg cgcctgcctg tattacccat cgtcttgagt tcgtgccgga cggtgacttt
    79621 tatcgctatg cgctgacaaa aataaactga gtcggtaatg ctgactgtac cggaggcggc
    79681 ctaagcgccc tctccggcct acagttcgac gcatccaggc atgaaaccgc gacttttttc
    79741 agataaaaag ctcaatcatt cataaaccct ctgttttata atccattaat ggcgcataaa
    79801 aaacggctaa attcttgtgt aaacgattcc actaatttat tccatgtcac acttttcgca
    79861 tctttgttat gctatggtta tttcatacca taagcctaat ggagcgaatt atgagagttc
    79921 tggttaccgg tggtagcggt tacattggaa gtcatacctg tgtgcaatta ctgcaaaacg
    79981 gtcatgatgt catcattctt gataacctct gtaacagtaa gcgcagcgta ctgcctgtta
    80041 tcgagcgttt aggcggcaaa catccaacgt ttgttgaagg cgatatccgt aacgaagcgt
    80101 tgatgaccga gatcctgcac gatcacgcta tcgacactgt gatccacttc gccgggctga
    80161 aagccgttgg cgaatcggta caaaaaccgc ttgaatatta cgacaacaat gtcaacggta
    80221 ccctgcgcct gattagcgcc atgcgcgccg cgaacgtcaa aaactttatt tttagctcct
    80281 ccgccaccgt ctatggcgat cagcccaaaa ttccatacgt tgaaagcttc ccgaccggca
    80341 caccgcaaag cccttacggc aaaagcaaac tgatggtgga acagatcctc accgatctgc
    80401 aaaaagccca gccggactgg agcattgccc tgctgcgcta cttcaacccg gttggcgcgc
    80461 atccgtcggg cgatatgggc gaagatccgc aaggcattcc gaataacctg atgccataca
    80521 tcgcccaggt tgctgtaggc cgtcgcgact cgctggcgat ttttggtaac gattatccga
    80581 ccgaagacgg tactggcgta cgcgattaca tccacgtaat ggatctggcg gacggtcacg
    80641 tcgtggcgat ggaaaaactg gcgaacaagc caggcgtaca catctataac ctcggcgctg
    80701 gcgtaggcag cagcgtgctg gacgtggtta atgccttcag caaagcctgc ggcaaaccgg
    80761 ttaattatca ttttgcaccg cgtcgcgagg gcgaccttcc ggcctactgg gcggacgcca
    80821 gcaaagccga ccgtgaactg aactggcgcg taacgcgcac actcgatgaa atggcgcagg
    80881 acacctggca ctggcagtca cgccatccac agggatatcc cgattaagga acgaccatga
    80941 cgcaatttaa tcccgttgat catccacatc gccgctacaa cccgctcacc gggcaatgga
    81001 ttctggtttc accgcaccgc gctaagcgcc cctggcaggg ggcgcaggaa acgccagcca
    81061 aacaggtgtt acctgctcat gatccagatt gcttcctctg cgcaggtaat gtgcgggtga
    81121 caggcgataa aaaccccgat tacaccggga cttacgtttt cactaatgac tttgcggctt
    81181 tgatgtctga cacaccagat gcgccggaaa gtcacgatcc gctgatgcgt tgccagagcg
    81241 cgcgtggcac cagtcgggtg atctgctttt caccggatca cagcaaaacg ttgccagaac
    81301 tcagcgttgc ggcattgacg gaaatcgtca aaacctggca ggagcaaacc gcagaactgg
    81361 ggaaaacgta cccatgggtg caggtctttg aaaacaaagg cgcggcgatg ggctgctcta
    81421 acccgcatcc gcacggacag atttgggcaa atagcttcct gcctaacgaa gctgagcgcg
    81481 aagaccgtct gcaaaaagaa tatttcgccg aacagaaatc gccaatgctg gtggattatg
    81541 ttcagcgcga gctggcagac ggtagccgta ccgttgtcga aaccgaacac tggttagccg
    81601 ttgtacctta ctgggctgcc tggccgttcg aaacgctact gctgcccaaa gcccacgttt
    81661 tacggatcac cgatttgacc gacgcccagc gcagcgattt ggcactggcg ttgaaaaagc
    81721 tgaccagtcg ttatgacaac ctcttccagt gctccttccc ctactctatg ggctggcacg
    81781 gcgcgccatt taatggcgaa gagaatcaac actggcagct gcacgcgcac ttttatccgc
    81841 ctctgttgcg ctctgccacc gtacgtaaat ttatggttgg ttatgaaatg ctggcagaaa
    81901 cccagcgaga cctgaccgca gaacaggccg cagagcgttt gcgcgcagtc agcgatatcc
    81961 attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa tctctgtttg
    82021 ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc gtgaatttga
    82081 ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt gattatcaaa
    82141 ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg cgtgatggca gccgattatg
    82201 aaaatcagct cgacgagttt tccctcgatg cgaccattgt cgcgcatgaa aactatcaat
    82261 gggctaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac agcttcggcg
    82321 gcgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc cgggttaagt tcttccgctt
    82381 cactggaagt cgcggtcgga accgtattgc agcagcttta tcatttgccg ctggacggcg
    82441 cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgttggctgt aactgcggga
    82501 tcatggatca gctaatttcc gcactcggca agaaagatca tgccttgctg attgactgcc
    82561 gctcactggg gactaaagca gtttccatgc cgaaaggtgt ggctgtcgtc atcattaaca
    82621 gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa cagtgcgaaa
    82681 ctggtgcgcg tttcttccag cagccagcgc tgcgcgatgt caccattgaa gagttcaacg
    82741 ctgttgcgca tgaactggac ccaatcgtgg cgaaacgcgt gcgtcatatc ctgactgaaa
    82801 acgcccgcac cgttgaagct gccagcgcac tggagcaagg cgacctgaaa cgtatgggcg
    82861 agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc gtgccgcaaa
    82921 ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc gtacgcatga
    82981 ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc ggaagagctg gtgcctgccg
    83041 tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag actttttacg
    83101 tttgtaaacc atcacaagga gcaggacagt gctgaacgaa actcccgcac tggcacccga
    83161 tggtcagccg taccgactgt taactttgcg taacaacgca gggatggtag tcacgctgat
    83221 ggactggggt gcgactttac tttccgcccg tattccgttt tccgatggca gcgtccgcga
    83281 ggcgctgctc ggctgtgcca gcccggaatg ctatcaggat caggccgcat ttctgggggc
    83341 ctctattggt cgttatgcca accgtatcgc caatagccgt tatacctttg acggtgaaac
    83401 cgtgacgctt tcaccaagtc agggcgttaa ccagctgcac ggcggcccgg aagggttcga
    83461 caaacgtcgc tggcagattg tgaaccagaa cgatcgtcag gtgctgtttg ccctgagttc
    83521 agatgatggc gatcagggct tcccgggtaa tctcggagcg acggtacaat atcgtctgac
    83581 cgacgataac cgtatctcta ttacttatcg cgccacagtt gataaacctt gcccggtgaa
    83641 tatgactaat cacgtctatt tcaatcttga cggcgagcag tctgacgtgc gcaatcacaa
    83701 gttgcagatt ctggcggacg aatatctgcc ggttgatgaa ggcggcattc cccacgacgg
    83761 cctgaaatct gtcgccggaa cgtcttttga tttccgcagc gccaaaatca tcgccagtga
    83821 gtttcttgcc gacgacgatc agcgcaaagt gaaaggttac gatcacgcgt tcttgttaca
    83881 ggccaaaggc gatggcaaga aagtggcggc gcatgtctgg tcagcagatg aaaaattgca
    83941 gctgaaggtc tacaccaccg ctccggctct gcaattctac tccggcaact acctcggcgg
    84001 cacaccgtcg cggggaaccg aaccttacac cgactggcaa gggctggcgt tggaaagcga
    84061 gtttctgccg gacagcccga accaccctga atggccgcaa ccggactgtt tcctgcgtcc
    84121 tggcgaagag tattccagcc tgacggaata tcagtttatt gctcagtaat gttaagccct
    84181 cgtcatgagg gctttatctt catattgttc aaatcaccag caaacaccga catatttgca
    84241 actcaatatt cacaacaacc ttacactgcg acactatttt cgctatggtt atgcgtaagc
    84301 attgctgttg cttcgtcacg gcaatataat gagaattatt atcattaaaa gatgatttga
    84361 ggagtaagta tatggctgta actaagctgg ttctggttcg tcatggcgaa agtcagtgga
    84421 acaaagaaaa ccgtttcacc ggttggtacg acgtggatct gtctgagaaa ggcgtaagcg
    84481 aagcaaaagc agcaggtaag ctgctgaaag aggaaggtta cagctttgac tttgcttaca
    84541 cctctgtgct gaaacgcgct atccataccc tgtggaatgt gctggacgaa ctggatcagg
    84601 catggctgcc cgttgagaaa tcctggaaac tgaacgaacg tcactacggt gcgttgcagg
    84661 gtctgaacaa agcagaaacc gctgaaaagt atggcgacga gcaggtgaaa cagtggcgtc
    84721 gtggttttgc agtgactccg ccggaactga ctaaagatga tgagcgttat ccgggtcacg
    84781 atccacgtta cgcgaagctg agcgagaaag aactgccact gacggaaagc ctggcgctga
    84841 ccattgaccg cgtgatccct tactggaatg aaaccattct gccgcgtatg aagagcggtg
    84901 agcgcgtaat catcgctgca cacggtaact cgttacgtgc gctggtgaaa tatcttgata
    84961 acatgagcga agaagagatt cttgagctga atatcccgac tggtgtgcca ctggtgtatg
    85021 agttcgacga gaatttcaaa ccgttgaaac gctattatct gggtaatgct gacgagatcg
    85081 cggcgaaagc agcggccgtt gcgaaccagg gtaaagcgaa gtaaacgtca ttcgcttaaa
    85141 aatgagaaag ccgactgcaa gtgagtcggc ttttttgttt gtcgttgcta acaatggagc
    85201 acattgcctg atgctacgct gcgcgtctta tcagggctgc aaacgtacag tcatggcgat
    85261 tcgactataa attacccgcg acgcgctttt actgcattcg ccagttgacg taacagagca
    85321 tcggtatcat cccagccaat gcaggcatcg gtgatgctct taccataggc cagcggttcc
    85381 ccactctcga ggctctgatt gccttccacc agatggcttt ccaccatcac gccaataata
    85441 gccttttcgc caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg
    85501 aattgttttg acgagttagc atggctgaaa tcgatcatca cctgtgctgg caggcctgct
    85561 ttgttcagcc cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg
    85621 ccgcgcagaa tgatatggca atcgccgtta ccgctggtat tcacaatcgc cgaatgcccc
    85681 catttcgtta cggacaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc
    85741 actttaatcg taccgtcggt gccatttttg aagccgaccg gacaagaaag ccctgatgcc
    85801 agttcgcggt gcacctgcga ttcggtggta cgtgcgccaa ttgcgcccca gctcatcagg
    85861 tcagcgagat attgtggggt gatcatatcc aggaattcac ccgccgctgg cagaccgctg
    85921 tcgttaatat caagcagcaa tttacgggca atacgcagac cgtcgttgat ctggaagctg
    85981 ttatccatat gcggatcgtt aatcagccct ttccagccca ccgtggtacg cggcttttca
    86041 aaatagacgc gcatcacgat ttccagctca tcttgcagct cttcacgcag cgtcagcaag
    86101 cgagtggcat actctttcgc cgcgacagga tcatgaattg agcacggacc aatcaccacc
    86161 aacaggcgat catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcgacc
    86221 gtattcgcgg cattttcagt agcggggaat ttttccagca atgcgactgg aggaagtaac
    86281 tctttgattt ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg
    86341 ccatacttat cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa
    86401 cggggtttta cactatgaac ggattaatcc tggaattagc aagaaaatcg ccagattgtc
    86461 gcgaaaaacg agatctctcc tacaatttct aactgtaact cctttgtttg ttaattattt
    86521 caagattctc tgctgggtat ctttgcctgg ctgaaatctt aaaccattgc cttatattca
    86581 cctgcaaatg cactgttgga agaggttatc cgacataacg accataacag gagcatccta
    86641 tgaaaatgac aaaactggcc acactatttt tgactgccac tctaagcctt gccagcggtg
    86701 ccgcactggc tgccgatagc ggagcgcaaa gcaataacgg ccaggcaaac gccgcagctg
    86761 atgcgggcca ggtagcccct gatgcccgtg aaaatgtcgc gccaaataac gtcgacaata
    86821 acggggtaaa taccggttct ggcggcacaa tgctgcatcc ggatggttct tcaatgaaca
    86881 atgacggcat gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgctgcc
    86941 cggatatcaa taaaaaagtc caaaccggtg atggcatcaa caatgatgtc gataccaaaa
    87001 ccgacggtac cacgcagtaa ccctgcgaaa tagccaataa tgggagagcg gagtctctcc
    87061 cttttaattt ttcccttcta aaagcagtat gatgtcataa agttaagtga gaataattaa
    87121 gggatgactc gtcatggcgc actcacactc acacacttcc tcacacctgc cagaagataa
    87181 taatgctcgt cgcttgttgt atgctttcgg cgtgactgcc gggtttatgc tcgtagaagt
    87241 cattggtggt tttctttccg gttctctggc attgctggcc gatgcgggtc atatgttgac
    87301 cgatactgcc gccctgcttt tcgccctact cgcagtgcaa ttttcccgtc gtcctcccac
    87361 cattcgccac actttcggtt ggctaagatt gaccacgctc gcggcgtttg tgaatgccat
    87421 cgccctggtg gtgattacta ttttgattgt ctgggaggcg atagaacgct tccgcactcc
    87481 gcgtccggtc gagggcggca tgatgatggc aattgccgtg gctgggctgc tggcaaatat
    87541 actttctttc tggttacttc atcacggcag cgaagagaaa aacctcaacg tgcgagcagc
    87601 ggcactgcat gtgctcgggg acctgctggg ttcggttggg gctattattg ccgcgttgat
    87661 tattatctgg accggctgga cacctgctga ccccattctc tcaatactgg tgtcgcttct
    87721 ggttctgcgt agcgcgtggc gactgttgaa agatagcgtg aatgaattac ttgaaggtgc
    87781 accggtatcg ctggatatcg ccgaactgaa gcgtcgtatg tgccgggaaa tcccggaagt
    87841 ccgcaatgtg caccatgtgc atgtatggat ggtaggcgag aagccggtga tgacgctgca
    87901 tgtgcaggtg atcccgccac acgatcacga tgccttgtta gatcagatcc aacactacct
    87961 gatggatcac tatcagattg agcacgccac tattcagatg gaatatcaac cttgtcatgg
    88021 gccagactgc cacctgaatg aaggggtgtc tggccattca catcatcacc attaatggga
    88081 cagcgcgcgt gaacctcttt cgcgtgcgct gttgatccac atccggctgc cgttgagggc
    88141 aataaaggtc aggatgatgt actccagaga catggcgtaa acgccctgaa gtgcaaaaat
    88201 aacgacgcta atcacgttaa taatcaccca caacagccag ttttccacat acttacgcgt
    88261 catcagaatc attgccacga tagataacac catcatgcat gaatcccaga acgggaaagc
    88321 gtccggctgt agttcaggca tcgcaacctg taatcctaat gcttgcatga tcatgactgc
    88381 cacgcgggtc aaaaatgcaa acaccggatt gataaatacc gtcatcagac caatcgaaac
    88441 aacgcaaacc gccaaccagc ttagcgcctt cggcaatggc aaccagcgaa ttttcaactc
    88501 cgcctcgttc tgactggttt gtcgcgacca cgcataccag ccgtaaatat tcgcggcaaa
    88561 gaaaaacatc tgtaatagca ggctggcata cagctgaatc tgaaagaaaa taatgccaaa
    88621 caaggtgacg ttgatcaggc caaagaagta gttgctgatc ttctccagac tggcaaggcc
    88681 aatacacagc aacccggcga tcgtgcctac cgcttcgatc catgagagat cataaccgcc
    88741 tgcacctatt ggtatatgta ccaggatatt ctgcacacta aaaaaatcca tctttccccc
    88801 aaagcgtaac gttacgcctt attattcgtt atccacgtag tgtagccgca aaatccagca
    88861 tacgattgag cggcaccagc gccctctctc gcagcctttc atcaacatga acctcgtgat
    88921 tgcttccttc ctgttctaat gcctctgcga tggcctgaag gccattcatg gccatccacg
    88981 gacaatgcgc gcagctgcgg caggttgcac cctcacctgc ggttggtgct tccagtaact
    89041 ctttaactgg caccgcctgc tgcattttgt agaaaatacc ccgatcggtt gccacaataa
    89101 gcctctgatg tggcaatgtt ttcgcagcag cgatcagttg actggtggaa ccgaccgcat
    89161 ccgccatctc tacaatagct tgcggtgatt ctggatgcac cagtatggca gcatccgggt
    89221 attcttcttg caagcgggtt aacgcctgag tcttaaactc atcatgcaca atgcaggcac
    89281 cctgccagca taatatgtct gcgcccgtct gtttttgcac gtaacgcccc agatgtttgt
    89341 cgggtgccca gatgattttt tcacccaaac tatccagatg atcaataagt tcgacggcaa
    89401 tgcttgaagt taccacccaa tctgcgcgcg cttttaccgc agcagaagtg ttggcgtaga
    89461 cgacgacagt acgatcggga tgggcatcgc aaaatgcgtt aaattcttca acagagcagc
    89521 cgagatccag tgaacattca gcctgaagtg tcggcatcag aattgttttt tccggactga
    89581 gaattttggc ggtttctccc ataaatctca ccccagcgac taacaacgta gaagcgggat
    89641 gctttgcacc gaagcgcgcc atttccagag aatcagaaat acagccaccg gtttcttctg
    89701 ccagttgttg aatttcggga tcggtatagt agtgggcaac catcaccgca ttacgttctt
    89761 ttagcagacg ttttatcttc tcgcggtaat acgctttttc atcaatgctt aacggcgtcg
    89821 gcttcggggg aaaaggataa atcgccgtgt ctggatcaaa catcacgctc atcttaccat
    89881 ctcgttttac aggcttaacg ttaaaaccga catgagcgta atattcgctg ttatcggtga
    89941 aatttgtttt atatgctaaa caagatagtc gaatggtggg gaaaagtcac tcgaattacg
    90001 cctaattcca ccgacgtaga gttgcttgca aagtaagctc tgaagatgtc atcaggttac
    90061 aaagtgaaat tgaggatgcc tgaatagcaa aaaagcgcct ttagggcgct tttttacatt
    90121 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
    90181 ctgagctaac gaccccttgc gggattatgc tgctttaatc attcaggatg gtgggtcgtg
    90241 caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg
    90301 acccatacgg gtgttacctg aaagatttct actcggaacc aatctttaaa attggtgggt
    90361 cgtgcaggat gactcgcttc gctcgcccta cgggccgttg ctggcgcaac gttatccttc
    90421 acgcttaaca tctgagtttg atgttaaaac tggtgggtcg tgcaggattc gaacctgcga
    90481 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgagt ggtgggtgat
    90541 gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat
    90601 cacccgatac tgcgctgaat actacaaact acttccaccg aacccaccgt aattagtggt
    90661 gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg
    90721 agctaacgac ccggtggtgg gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg
    90781 gaggtgctct cccagctgag ctaatcaccc gatactacgc tggatactac aactaactag
    90841 gttagtggtg ggtcgtgcag gatgactcga cttcgtctcg cccttcgggc cgttgctgac
    90901 gcaacgttat ccttcacgtt ttactaccgc ttccacctta gcgattggtg ggtcgtgcag
    90961 gattcgaacc tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc
    91021 cactttttcg ttgctttcgg tttgtttgat atcccgtggc aacggcggca tatattactg
    91081 atttcagact tgagcgcaac aaaaatttcg atgcagatca ctcaactgct tatgattcgc
    91141 acgacacgac cagaaataat gcgacttctg gtcgtgtgtt atgcattaca tcgcgttcag
    91201 acgtttttgc gcctgtttag cgccatcagt accagggtat ttactgataa cctgctggta
    91261 tacggctttc gcttttgcgg tgtcaccttt gtcctgcatg atgacgccga ctttaaacat
    91321 cgcatctgca gccttcggtg acttcggata gtttttcact accgaagcaa aataatacgc
    91381 cgcatcatct tttttaccct tgttgtagtt taactgaccc agccaataat tggcgtttgg
    91441 caggtaagtt gaatcagggt aatttttgat gaaattctga aatgccacta ttgcttcatc
    91501 ctggcgggat ttatcctgca ccagcgcaat tgctgcattg taatccgtgt ttgcatcgcc
    91561 gcttttcacc ggcgcgccag cattcgcagt accagcatca gctgtcggcg tcgttgatgc
    91621 cgccgcaccg ctttgatcac cgctggttga ttgcgccgct gcaccaccgc tgctgaggct
    91681 atcgatctgc aacaggatct gcttttgccg ctccacgacc tgattcagtt gatactgatt
    91741 ttcctgaatc tgaccgcgca gggaatcaat atcggattga ttatcagaga gttgttgctg
    91801 gagttgggtt aaaagctggc tgtgagcatt tgaaatacgc tcaagttgag tgacgcggtc
    91861 ttcgaccgag cctgagccga cactactgat tggtgcctga gcaaaagcgg cccagggggc
    91921 cgctatacca accagtaacg acagactcaa tagttgatgt ctgaagttac tgctcatgca
    91981 attctcttag taaaccagta ccgcacgacg gtttttggag tatgccgctt cgtcatgacc
    92041 cagtactgca ggtttttctt taccgtaaga aacgatggag atctggtctg cagaaacgcc
    92101 tttaccctgc aggtacatct taacggcgtt cgcacgacgt tcacccaggg agatgttgta
    92161 ttccggagta ccacgttcgt ccgcgtgacc ttctacggtg actttgtaag acgggttgct
    92221 acgcaggaag tttgcatgtg catccagcat ttgagcgaag tcagaacgga tatcgtactt
    92281 gtccagatcg aagtaaacga tgttgttctg ctgcagctgt tgcatttgca gacgagcctg
    92341 ctcttcggaa gacatgttgc cgttgccgcc gttcgcatcc ataccagtgc cggcacccag
    92401 catgccttcg ctgccgtcat tgctggcgtt cttattggaa gaacatgccg caattgccat
    92461 aacaggcaga gcaatcatca gccctttcag cactttgttc agttgcattt caatgattcc
    92521 tttactattc aattaattat tatcacagat acggcgacca ggcagggaat ttgacctgtc
    92581 catcagttgc cggaagacgc gctttgaaac gcccatctgt agaaaccaaa ttcagcacgg
    92641 atcccatccc ctgagaagag ctgtagatta ccatagtgcc gttaggtgcc agacttggcg
    92701 tttcatccag gaacgtggac gacagaactt gtacgcctcc cgttgccaga tcttgtttgg
    92761 caatgtgctg ctgcccacca ttggagctga ccattaccat aaatttaccg tcgctgctga
    92821 catccgcatc ctggttctgc gaaccttccc aggtaatacg ttgtggcgca ccgccgttga
    92881 tattcacttt ataaacctgt ggacgaccgg cctggtcaga agtaaatgcc aggttctggc
    92941 tgtccgggaa ccaggtcggt tcggtattgt tactgcgacc atccgtcacc tggcggatct
    93001 gaccagaagc caaatccatt acgtacaggt tcagactacc ggttttcgac aaggcgaatg
    93061 ccagtttgct gccgtctggc gagaatgcag gtgcaccgtt gtgacgcggg aatgaagcca
    93121 cctgacgtac agcgccattt gccagcgttt ggatgaccag cgcggaacga ccgctttcga
    93181 aggtcacata agccagttta gaaccgtctg gtgaccacgc cggagacatc agcggctgcg
    93241 gtgaacggtg aacgacaaac tggttgtaac cgtcatagtc agatacgcgc agttcatacg
    93301 ggaactgacc gccgttggtc tgaacaacgt aggcaatacg ggtacggaac gcacctttaa
    93361 tgccggtcag tttttcaaac acttcatcac tggcggtatg accagcataa cgcagccact
    93421 gcttgttcac tttgtacgag ttctgagcaa gtacagtacc cggtgcgccg ccagtgtcaa
    93481 caagttgata agcaacattg taagaaccat ccggattcgg agtgacctga ccgacaacta
    93541 cagcgtcaat acccagcgcg gaccatgcag ctggttgtac ttcctgcgca ctacccggct
    93601 gctgtggcag acgagcgcga tctaacggat taaatttacc gctgttacgc aagtcggcag
    93661 caacgatgcc gccaatatct tcaggtgccg caccagggcc cgcccactgg aaaggaacaa
    93721 ctccaatagg acgaccggaa tctacaccgc tgtcgatcac aatgcggact tcagcatgca
    93781 gaactgatgc ccacagtatg agaaaaccaa atgctactcg taatgcctgc ttcatcatat
    93841 ctcccttatc tggaccgatg gcccacgata atttagcaga atgttaacaa actaaaatac
    93901 acaaaactac cagaaccccg tggcaaccgg tgcctgatgt tgaccgtcgg aacagtcaac
    93961 atcgcgatta cggtttgaag tccaatggcg cgtttttgaa cacttcatat actgcctggc
    94021 ttggtggttt cgggatcttc gcaagtttag ctgctgccaa cgcagcctga caaagtgcgg
    94081 gatcgccacc ttcaggtttg atatccagta acataccatc gggagccagt tttatgcgca
    94141 gcgtacaggt tttgcctgca taggacgatg cgtcatagaa cttactttcg atagcagatt
    94201 taatctgccc ggcatagtta ttgatatcgg cccctgatgc gccattgttt ttagtattac
    94261 cactcccggc tggcgaagca ttgttccctt tcgcccctcc ccccgttttc ggtgcattct
    94321 taccagagct tagctcaccg aaaatatcat ctgcctctgc ggcagctttt gctgcagcgg
    94381 ctttttcagc tgctgctttt gcagctgccg cttttttgtc tgcggcggct ttttctgccg
    94441 ctgctttctt atcagctgct gccttttcag cagccgcttt ttctgctgct tttttgtcgg
    94501 ctgcagcttt ctctgctgcc gctttcttat cagctgcagc cttttcagca gccgctttct
    94561 tctcagcttc tgctttggct ttttcagcag cttcagttgc cgctttcttt cttgcttcag
    94621 ctgcagctgc ttctgccgct tccgctttct tcttcagtgc cgccgcggct gcctcggctt
    94681 ttttctgcgc ttcggctgcg gctttggcgg cttctgcttc tgcttttttc ttcgcgtctg
    94741 cagccgcttt cttcgcagct tcttccgcag ctttagcatc cgcttcggcc ttcgctttag
    94801 catctgccgc cgctttcgct gccgcttctt ccgcttgctt ctgctttaac tcggcctgtt
    94861 ttgcggcttc ttcagcctgc tttttctgct cctgagcagc taaccgctct ttctcaagtt
    94921 gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ctcttccgca gcctgctgtt
    94981 ccttcatctt gcgctgctca tcagaacgct tcgcgcttga ttcctggctt tgcatgcgtt
    95041 tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggat gaaccaccgc
    95101 cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc gccgcaaata
    95161 agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg
    95221 ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc
    95281 aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt cacacccgca
    95341 ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt tgcgccaccg
    95401 atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc caccacctgc
    95461 tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg accaatacca
    95521 gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga ttcagtagcg
    95581 tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat aaagatcagc
    95641 aacagcacca gcagtacgtc cagcaacggt acaatgttga tttcggactt gagatcgcga
    95701 cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct aacggtaaac
    95761 gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt cagttccagt
    95821 ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc ggcgaacaga
    95881 ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg cagtgttgct
    95941 tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt accaaacaga
    96001 ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt
    96061 tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac tacggcttcc
    96121 ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct gtaaaagatt
    96181 tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag gcgagagagt
    96241 tcgattccag accagaattt atcttcaaac gcttcggctt cacgcgccgc tgcgttaaga
    96301 atacgggtcc gctggataat aatggcccag gatgcgattg aaaaaccaat caaaatcaac
    96361 atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt catgtcagtc
    96421 actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt ttgagtgggt
    96481 caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg ttgacaatac
    96541 gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt atttcgagca
    96601 tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc acaaaggcaa
    96661 cgcgttcagc catcagcgcc tgttgactga agtgatgatg acgcagcatc tctgtgcgtg
    96721 ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca tcggtatctt
    96781 catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat cccggtaatg
    96841 caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag gtttgggaat
    96901 gagggcaagt taagggagcg taaaaattta tgggcctgga gcggcccata attttttaaa
    96961 gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa tatcggcaag
    97021 caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca cgccgtgaat
    97081 cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca gttcactggt
    97141 cttcgcggcg aaacgtgatg ggtcccaaaa catacatcct gctaacagaa gcgccatcac
    97201 gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggttg cgataatctt
    97261 actcatcaga tgtcttcttg accggctttg cctgattcga cgtgctcaag cgccagcgcg
    97321 gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa ataccacatt
    97381 tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct ttggtgatac
    97441 gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt accagaacca
    97501 ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc cacattgtca
    97561 gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg ataccggctg
    97621 tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcc gctttatcca
    97681 tacgtgcagt caggatggtc agcagcggca gaaccacacc cagtgccgga atagcccaca
    97741 gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc acttctttat
    97801 tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa ccatcgatac
    97861 cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagt gccgccacct
    97921 gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc agataggttg
    97981 cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg aacgggttaa
    98041 gcaggtggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg ttgaacggta
    98101 cgccctgcaa caggttaccg aacgctacac caatcaccaa cggcggaacg aagctaccaa
    98161 tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg gagcggtagt
    98221 caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc acatagaagc
    98281 cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg cccgcggtga
    98341 taagccatac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata attcgacgct
    98401 cggtgtcgtt acgaccgagg aaacgagtga gcatgcccac ccccatgtcg aaaccgtcag
    98461 tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa cgcaatactt
    98521 cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact
    98581 gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca aatttgaaca
    98641 ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc accattgaga
    98701 agatgagatc gcctgcggtc agtgacgagt tcgccacagc tgtcggcagc acttcaccga
    98761 tagcccacgg ttggcgacca tattcagcca cgaaccagcc cgcttctaca gcaatccacg
    98821 gcagcggaat accgtacagc gcggcgcgca gcagccattt tttctcgcca atgcggttgc
    98881 ggatgacact ccagaaagag agcgcgatga ttgccagcag caggaagcca cacgccacca
    98941 tgatacggaa cgcgaagtac agcggcgcta cacgcggaat ggagtctttg gttgcctgtt
    99001 gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc agcagaccgt
    99061 aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgcctggtcg gtagaaccag
    99121 aacgcagttg ctcgagcaga gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct
    99181 gcaccatcag ttctttcagg ccgataactg gagtatccac ggaacgcgtt gcaatgatgc
    99241 ccagcgcgta agggatctgg atcgcaaatt tgttcgtctc ctcttcctga tcaggaatgc
    99301 cgaacagagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca atagcagcca
    99361 gtttggtttt ctgcacgtcg cccatttcgt aaccagattc atcacccaga acaataacag
    99421 acagaacggc agccataccg aagctggcag cgatagcaaa agagcgttta gcgaaggcga
    99481 agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc gcgccagtca
    99541 cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta agcaccagct
    99601 cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac gcgattgggt
    99661 tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa ccgagcgcca
    99721 ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg aagaagaaca
    99781 gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc agcggcgcac
    99841 cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac tggaactcca
    99901 tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg ccccagaact
    99961 tggtcatatc tttataaatc tgtttgccgg aaaggacgta gaccgtttcc ataatggcca
   100021 gcaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg
   100081 caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc tcatcgcatg
   100141 aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat acaaaattat
   100201 ttgctcgcct ctatcgttgc catttcatca aaagaggaaa agacaatgat aaaaaagtta
   100261 caaatgtgtt aatgaataaa ccaaattgat cccgtaatta tattacgccg caaaatcctt
   100321 acaataaaca ggtttttatt gagcaaattt gcatttttcg acagtgatca atttatagcg
   100381 aaattgtcac gatctcacca atttgatcta cgacaa
//
LOCUS       DAZGTJ010000007        84955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000007 DAZGTJ010000000
VERSION     DAZGTJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.007"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1072
                     /gene="ompN"
                     /locus_tag="ACHK8E_000664"
     CDS             <1..1072
                     /gene="ompN"
                     /locus_tag="ACHK8E_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP0342974.1"
                     /translation="AEVYNKDGNKLDLYGKVDGLHYFSDNSAKDGDQSYARLGFKGET
                     QINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGLKFADYGSFDYGRNYGVMYDIE
                     GWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAVQYQGNNEGASN
                     GQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQVNHTAAGGDKAD
                     AWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQYQFDFGLRPAV
                     SFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATYYFNKNMSTYVDYKINLLDEDD
                     SFYAANGISTDDIVALGLVYQF"
     gene            1213..1647
                     /gene="uspF"
                     /locus_tag="ACHK8E_000665"
     CDS             1213..1647
                     /gene="uspF"
                     /locus_tag="ACHK8E_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP0342975.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1823..2758
                     /gene="ttcA"
                     /locus_tag="ACHK8E_000666"
     CDS             1823..2758
                     /gene="ttcA"
                     /locus_tag="ACHK8E_000666"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP0342976.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2887..4260)
                     /gene="dbpA"
                     /locus_tag="ACHK8E_000667"
     CDS             complement(2887..4260)
                     /gene="dbpA"
                     /locus_tag="ACHK8E_000667"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP0342977.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4290..4463)
                     /gene="ynaL"
                     /locus_tag="ACHK8E_000668"
     CDS             complement(4290..4463)
                     /gene="ynaL"
                     /locus_tag="ACHK8E_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP0342978.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4738..5721)
                     /gene="zntB"
                     /locus_tag="ACHK8E_000669"
     CDS             complement(4738..5721)
                     /gene="zntB"
                     /locus_tag="ACHK8E_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP0342979.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5735..5782)
                     /gene="ydaN"
                     /locus_tag="ACHK8E_000670"
     CDS             complement(5735..5782)
                     /gene="ydaN"
                     /locus_tag="ACHK8E_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP0342980.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5976..7208
                     /gene="dgcM"
                     /locus_tag="ACHK8E_000671"
     CDS             5976..7208
                     /gene="dgcM"
                     /locus_tag="ACHK8E_000671"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP0342981.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7229..7792)
                     /gene="smrA"
                     /locus_tag="ACHK8E_000672"
     CDS             complement(7229..7792)
                     /gene="smrA"
                     /locus_tag="ACHK8E_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP0342982.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7987..9501)
                     /locus_tag="ACHK8E_000673"
     CDS             complement(7987..9501)
                     /locus_tag="ACHK8E_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFP0342983.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VVIFSTVYLILVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(9498..10526)
                     /locus_tag="ACHK8E_000674"
     CDS             complement(9498..10526)
                     /locus_tag="ACHK8E_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0342984.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            10623..11438
                     /locus_tag="ACHK8E_000675"
     CDS             10623..11438
                     /locus_tag="ACHK8E_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0342985.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(11612..11828)
                     /locus_tag="ACHK8E_000676"
                     /pseudo
     CDS             complement(11612..11828)
                     /locus_tag="ACHK8E_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11828..11971
                     /locus_tag="ACHK8E_000677"
                     /pseudo
     CDS             11828..11971
                     /locus_tag="ACHK8E_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            11982..12497
                     /gene="ogt"
                     /locus_tag="ACHK8E_000678"
     CDS             11982..12497
                     /gene="ogt"
                     /locus_tag="ACHK8E_000678"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP0342986.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            12692..13444
                     /gene="fnr"
                     /locus_tag="ACHK8E_000679"
     CDS             12692..13444
                     /gene="fnr"
                     /locus_tag="ACHK8E_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP0342987.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            13596..14546
                     /gene="uspE"
                     /locus_tag="ACHK8E_000680"
     CDS             13596..14546
                     /gene="uspE"
                     /locus_tag="ACHK8E_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP0342988.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(14596..14853)
                     /gene="ynaJ"
                     /locus_tag="ACHK8E_000681"
     CDS             complement(14596..14853)
                     /gene="ynaJ"
                     /locus_tag="ACHK8E_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP0342989.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            15097..16128
                     /gene="ynaI"
                     /locus_tag="ACHK8E_000682"
     CDS             15097..16128
                     /gene="ynaI"
                     /locus_tag="ACHK8E_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP0342990.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(16179..17792)
                     /gene="mppA"
                     /locus_tag="ACHK8E_000683"
     CDS             complement(16179..17792)
                     /gene="mppA"
                     /locus_tag="ACHK8E_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP0342991.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(18129..19028)
                     /gene="pgrR"
                     /locus_tag="ACHK8E_000684"
     CDS             complement(18129..19028)
                     /gene="pgrR"
                     /locus_tag="ACHK8E_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP0342992.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            19166..20086
                     /gene="ycjY"
                     /locus_tag="ACHK8E_000685"
     CDS             19166..20086
                     /gene="ycjY"
                     /locus_tag="ACHK8E_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0342993.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            20157..20795
                     /locus_tag="ACHK8E_000686"
     CDS             20157..20795
                     /locus_tag="ACHK8E_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0342994.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            21086..21814
                     /gene="mpaA"
                     /locus_tag="ACHK8E_000687"
     CDS             21086..21814
                     /gene="mpaA"
                     /locus_tag="ACHK8E_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP0342995.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(21789..22754)
                     /gene="ycjG"
                     /locus_tag="ACHK8E_000688"
     CDS             complement(21789..22754)
                     /gene="ycjG"
                     /locus_tag="ACHK8E_000688"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP0342996.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            22873..23379
                     /gene="tpx"
                     /locus_tag="ACHK8E_000689"
     CDS             22873..23379
                     /gene="tpx"
                     /locus_tag="ACHK8E_000689"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP0342997.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(23423..24964)
                     /gene="tyrR"
                     /locus_tag="ACHK8E_000690"
     CDS             complement(23423..24964)
                     /gene="tyrR"
                     /locus_tag="ACHK8E_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP0342998.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(25112..26173)
                     /gene="ycjF"
                     /locus_tag="ACHK8E_000691"
     CDS             complement(25112..26173)
                     /gene="ycjF"
                     /locus_tag="ACHK8E_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP0342999.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(26170..27567)
                     /gene="ycjX"
                     /locus_tag="ACHK8E_000692"
     CDS             complement(26170..27567)
                     /gene="ycjX"
                     /locus_tag="ACHK8E_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP0343000.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            27722..28720
                     /gene="ycjW"
                     /locus_tag="ACHK8E_000693"
     CDS             27722..28720
                     /gene="ycjW"
                     /locus_tag="ACHK8E_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0343001.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            complement(28771..29676)
                     /gene="ompG"
                     /locus_tag="ACHK8E_000694"
     CDS             complement(28771..29676)
                     /gene="ompG"
                     /locus_tag="ACHK8E_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP0343002.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(29721..30803)
                     /locus_tag="ACHK8E_000695"
     CDS             complement(29721..30803)
                     /locus_tag="ACHK8E_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0343003.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(30817..31476)
                     /gene="pgmB"
                     /locus_tag="ACHK8E_000696"
     CDS             complement(30817..31476)
                     /gene="pgmB"
                     /locus_tag="ACHK8E_000696"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP0343004.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(31473..33740)
                     /gene="ycjT"
                     /locus_tag="ACHK8E_000697"
     CDS             complement(31473..33740)
                     /gene="ycjT"
                     /locus_tag="ACHK8E_000697"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP0343005.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(33737..34792)
                     /gene="ycjS"
                     /locus_tag="ACHK8E_000698"
     CDS             complement(33737..34792)
                     /gene="ycjS"
                     /locus_tag="ACHK8E_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0343006.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(34802..35590)
                     /gene="ycjR"
                     /locus_tag="ACHK8E_000699"
     CDS             complement(34802..35590)
                     /gene="ycjR"
                     /locus_tag="ACHK8E_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0343007.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(35609..36661)
                     /gene="ycjQ"
                     /locus_tag="ACHK8E_000700"
     CDS             complement(35609..36661)
                     /gene="ycjQ"
                     /locus_tag="ACHK8E_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP0343008.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(36692..37534)
                     /gene="ycjP"
                     /locus_tag="ACHK8E_000701"
     CDS             complement(36692..37534)
                     /gene="ycjP"
                     /locus_tag="ACHK8E_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0343009.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(37521..38402)
                     /gene="ycjO"
                     /locus_tag="ACHK8E_000702"
     CDS             complement(37521..38402)
                     /gene="ycjO"
                     /locus_tag="ACHK8E_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0343010.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(38423..39715)
                     /gene="ycjN"
                     /locus_tag="ACHK8E_000703"
     CDS             complement(38423..39715)
                     /gene="ycjN"
                     /locus_tag="ACHK8E_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0343011.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            complement(39729..41408)
                     /gene="ycjM"
                     /locus_tag="ACHK8E_000704"
     CDS             complement(39729..41408)
                     /gene="ycjM"
                     /locus_tag="ACHK8E_000704"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFP0343012.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYLIYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(41622..41936)
                     /gene="pspE"
                     /locus_tag="ACHK8E_000705"
     CDS             complement(41622..41936)
                     /gene="pspE"
                     /locus_tag="ACHK8E_000705"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0343013.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(42011..42232)
                     /gene="pspD"
                     /locus_tag="ACHK8E_000706"
     CDS             complement(42011..42232)
                     /gene="pspD"
                     /locus_tag="ACHK8E_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP0343014.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(42241..42600)
                     /gene="pspC"
                     /locus_tag="ACHK8E_000707"
     CDS             complement(42241..42600)
                     /gene="pspC"
                     /locus_tag="ACHK8E_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP0343015.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(42600..42824)
                     /gene="pspB"
                     /locus_tag="ACHK8E_000708"
     CDS             complement(42600..42824)
                     /gene="pspB"
                     /locus_tag="ACHK8E_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP0343016.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(42878..43546)
                     /gene="pspA"
                     /locus_tag="ACHK8E_000709"
     CDS             complement(42878..43546)
                     /gene="pspA"
                     /locus_tag="ACHK8E_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP0343017.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            43713..44690
                     /gene="pspF"
                     /locus_tag="ACHK8E_000710"
     CDS             43713..44690
                     /gene="pspF"
                     /locus_tag="ACHK8E_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP0343018.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(44810..46075)
                     /gene="puuE"
                     /locus_tag="ACHK8E_000711"
     CDS             complement(44810..46075)
                     /gene="puuE"
                     /locus_tag="ACHK8E_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP0343019.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(46113..47393)
                     /gene="puuB"
                     /locus_tag="ACHK8E_000712"
     CDS             complement(46113..47393)
                     /gene="puuB"
                     /locus_tag="ACHK8E_000712"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP0343020.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(47395..48882)
                     /gene="puuC"
                     /locus_tag="ACHK8E_000713"
     CDS             complement(47395..48882)
                     /gene="puuC"
                     /locus_tag="ACHK8E_000713"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP0343021.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(49032..49589)
                     /gene="puuR"
                     /locus_tag="ACHK8E_000714"
     CDS             complement(49032..49589)
                     /gene="puuR"
                     /locus_tag="ACHK8E_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP0343022.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(49616..50380)
                     /gene="puuD"
                     /locus_tag="ACHK8E_000715"
     CDS             complement(49616..50380)
                     /gene="puuD"
                     /locus_tag="ACHK8E_000715"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP0343023.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            50592..52010
                     /gene="puuA"
                     /locus_tag="ACHK8E_000716"
     CDS             50592..52010
                     /gene="puuA"
                     /locus_tag="ACHK8E_000716"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP0343024.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            52159..52323
                     /locus_tag="ACHK8E_000717"
     CDS             52159..52323
                     /locus_tag="ACHK8E_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343025.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            52313..53698
                     /gene="puuP"
                     /locus_tag="ACHK8E_000718"
     CDS             52313..53698
                     /gene="puuP"
                     /locus_tag="ACHK8E_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP0343026.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            53832..54077
                     /gene="ymjA"
                     /locus_tag="ACHK8E_000719"
     CDS             53832..54077
                     /gene="ymjA"
                     /locus_tag="ACHK8E_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP0343027.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            54390..56033
                     /gene="sapA"
                     /locus_tag="ACHK8E_000720"
     CDS             54390..56033
                     /gene="sapA"
                     /locus_tag="ACHK8E_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP0343028.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            56030..56995
                     /gene="sapB"
                     /locus_tag="ACHK8E_000721"
     CDS             56030..56995
                     /gene="sapB"
                     /locus_tag="ACHK8E_000721"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP0343029.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            56982..57872
                     /gene="sapC"
                     /locus_tag="ACHK8E_000722"
     CDS             56982..57872
                     /gene="sapC"
                     /locus_tag="ACHK8E_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP0343030.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            57872..58864
                     /gene="sapD"
                     /locus_tag="ACHK8E_000723"
     CDS             57872..58864
                     /gene="sapD"
                     /locus_tag="ACHK8E_000723"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP0343031.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            58866..59672
                     /gene="sapF"
                     /locus_tag="ACHK8E_000724"
     CDS             58866..59672
                     /gene="sapF"
                     /locus_tag="ACHK8E_000724"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP0343032.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(59721..60884)
                     /locus_tag="ACHK8E_000725"
     CDS             complement(59721..60884)
                     /locus_tag="ACHK8E_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP0343033.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(60893..62266)
                     /locus_tag="ACHK8E_000726"
     CDS             complement(60893..62266)
                     /locus_tag="ACHK8E_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP0343034.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(62270..65376)
                     /locus_tag="ACHK8E_000727"
                     /pseudo
     CDS             complement(62270..65376)
                     /locus_tag="ACHK8E_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(65376..66497)
                     /locus_tag="ACHK8E_000728"
     CDS             complement(65376..66497)
                     /locus_tag="ACHK8E_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0343035.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(66646..67212)
                     /locus_tag="ACHK8E_000729"
     CDS             complement(66646..67212)
                     /locus_tag="ACHK8E_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0343036.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            67690..68478
                     /gene="fabI"
                     /locus_tag="ACHK8E_000730"
     CDS             67690..68478
                     /gene="fabI"
                     /locus_tag="ACHK8E_000730"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP0343037.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            68622..69749
                     /gene="yciW"
                     /locus_tag="ACHK8E_000731"
     CDS             68622..69749
                     /gene="yciW"
                     /locus_tag="ACHK8E_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP0343038.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            69817..71751
                     /gene="rnb"
                     /locus_tag="ACHK8E_000732"
     CDS             69817..71751
                     /gene="rnb"
                     /locus_tag="ACHK8E_000732"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP0343039.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(71828..71932)
                     /locus_tag="ACHK8E_000733"
     CDS             complement(71828..71932)
                     /locus_tag="ACHK8E_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343040.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            71986..73971
                     /gene="pdeR"
                     /locus_tag="ACHK8E_000734"
     CDS             71986..73971
                     /gene="pdeR"
                     /locus_tag="ACHK8E_000734"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0343041.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(73953..74081)
                     /gene="ymiD"
                     /locus_tag="ACHK8E_000735"
     CDS             complement(73953..74081)
                     /gene="ymiD"
                     /locus_tag="ACHK8E_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP0343042.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIITKYIAKYIYSSTYARFR"
     gene            74119..74292
                     /gene="yciZ"
                     /locus_tag="ACHK8E_000736"
     CDS             74119..74292
                     /gene="yciZ"
                     /locus_tag="ACHK8E_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP0343043.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            74382..75131
                     /gene="yciT"
                     /locus_tag="ACHK8E_000737"
     CDS             74382..75131
                     /gene="yciT"
                     /locus_tag="ACHK8E_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP0343044.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            75400..75618
                     /gene="osmB"
                     /locus_tag="ACHK8E_000738"
     CDS             75400..75618
                     /gene="osmB"
                     /locus_tag="ACHK8E_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP0343045.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(75744..76070)
                     /gene="yciH"
                     /locus_tag="ACHK8E_000739"
     CDS             complement(75744..76070)
                     /gene="yciH"
                     /locus_tag="ACHK8E_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP0343046.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(76070..76807)
                     /gene="pyrF"
                     /locus_tag="ACHK8E_000740"
     CDS             complement(76070..76807)
                     /gene="pyrF"
                     /locus_tag="ACHK8E_000740"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP0343047.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(76998..78167)
                     /gene="lapB"
                     /locus_tag="ACHK8E_000741"
     CDS             complement(76998..78167)
                     /gene="lapB"
                     /locus_tag="ACHK8E_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP0343048.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(78174..78482)
                     /gene="lapA"
                     /locus_tag="ACHK8E_000742"
     CDS             complement(78174..78482)
                     /gene="lapA"
                     /locus_tag="ACHK8E_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP0343049.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(78631..79395)
                     /gene="pgpB"
                     /locus_tag="ACHK8E_000743"
     CDS             complement(78631..79395)
                     /gene="pgpB"
                     /locus_tag="ACHK8E_000743"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP0343050.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            79565..80155
                     /gene="ribA"
                     /locus_tag="ACHK8E_000744"
     CDS             79565..80155
                     /gene="ribA"
                     /locus_tag="ACHK8E_000744"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP0343051.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(80219..82894)
                     /gene="acnA"
                     /locus_tag="ACHK8E_000745"
     CDS             complement(80219..82894)
                     /gene="acnA"
                     /locus_tag="ACHK8E_000745"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP0343052.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(83044..83139)
                     /gene="ymiC"
                     /locus_tag="ACHK8E_000746"
     CDS             complement(83044..83139)
                     /gene="ymiC"
                     /locus_tag="ACHK8E_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP0343053.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(83253..83420)
                     /gene="yciX"
                     /locus_tag="ACHK8E_000747"
     CDS             complement(83253..83420)
                     /gene="yciX"
                     /locus_tag="ACHK8E_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP0343054.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            complement(83423..83563)
                     /gene="ymiA"
                     /locus_tag="ACHK8E_000748"
     CDS             complement(83423..83563)
                     /gene="ymiA"
                     /locus_tag="ACHK8E_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP0343055.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(83882..84856)
                     /gene="cysB"
                     /locus_tag="ACHK8E_000749"
     CDS             complement(83882..84856)
                     /gene="cysB"
                     /locus_tag="ACHK8E_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP0343056.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
BASE COUNT        21099 a        22159 c        20531 g        21166 t
ORIGIN      
        1 agccgaagtt tataataaag acggcaacaa attagatctg tatggcaaag ttgatggcct
       61 gcattatttt tctgataatt cagcgaaaga tggcgaccag agctatgcgc gtctgggttt
      121 taaaggcgaa acccaaatta acgatcaact cactggctac ggtcaatggg aatacaatat
      181 tcaggcaaac aacactgaat cttcaacaaa ccagtcatgg acccgtctgg catttgccgg
      241 actgaaattt gcagattacg gttctttcga ttacggacgt aattatggcg taatgtacga
      301 catcgaaggc tggaccgata tgctgcctga atttggcggt gactcttata ccaatgcaga
      361 caactttatg actggtcgtg ccaacggcgt cgcgacttat cgtaatactg atttcttcgg
      421 tctggtaaat ggtctgaact tcgcggtgca gtatcaaggt aacaacgaag gagccagtaa
      481 tggtcaggaa ggcaccaaca acggacgtga tgttcgccat gaaaacggtg acggctgggg
      541 tctttccaca acatatgatt taggcatggg ctttagcgct ggtgcggcat acacctcttc
      601 tgaccgcacc aatgaccagg ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg
      661 gactgctggg ctaaaatacg atgctaacaa tatttacctg gcaaccatgt attcagaaac
      721 gcgtaacatg accccgtttg gcgacagcga ttatgcagtt gcgaacaaaa cccagaactt
      781 tgaagttact gcacagtacc agtttgattt tggtctgcgt ccggcagtct ctttcctgat
      841 gtctaaaggc cgtgacctgc acgctgccgg tggtgcagac aacccggcag gtgttgatga
      901 taaagatctg gttaaatacg ccgatgttgg cgcgacttac tatttcaaca aaaatatgtc
      961 cacctacgtt gactataaaa taaacctgtt ggatgaagat gacagcttct acgctgccaa
     1021 cggcatctct accgatgata ttgtcgcttt aggtctggtt tatcagttct aaatcctcct
     1081 gcccgctgtt atggcgggct ttttctgctt attcttcctc ctttgatcta aattaaaaat
     1141 gtgaactccg tcattacaca aaaagtgtca tctggcgtta cactttatgc ggatactaaa
     1201 acaggaggtt ttatgaacag aacgattctt gtccctatcg atatttccga ttcagaatta
     1261 actcaacgcg tgattagcca cgttgaggca gaggcaaaga ttgatgatgc agaggttcat
     1321 ttcctgacgg taataccttc actgccctac tatgcctctc tgggtttagc gtattccgca
     1381 gaattaccgg caatggatga cctgaaagcg gaagccaaat cgcaactgga agagatcatt
     1441 aaaaaattta aactgccaac cgacagagtg catgtccatg ttgaggaagg ctcgcccaaa
     1501 gaccgcattc tggaattggc gaagaagatc cccgctcata tgatcatcat tgcttcccat
     1561 cgaccggata tcaccactta tctgctcggt tctaacgccg cagcggtagt gcgtcacgca
     1621 gagtgctccg tgctggttgt gcgctgacat taacgcccgc acattgctgc gggctttttg
     1681 attcatttcg caaatgtgct gacattttcc cctctaatcc gtaccataca cgccacagtt
     1741 tttatatcag atttcttatg ctgggcgttc cggcatgacg catactcttc tgatgccata
     1801 taacgaattg agtcgctttt aaatgtcgca aaatcaagaa attagtaaga aagaacaata
     1861 caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg ggcgaagcca ttgctgactt
     1921 caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tccgggggta aagacagcta
     1981 taccatgctg gaaattctgc gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt
     2041 ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc ccgagtatct
     2101 tgaaacgctg ggcgttgagt acaagattgt ggaagagaac acttacggta tcgtgaaaga
     2161 gaagattccg gagggcaaaa ccacttgctc actgtgttct cgtcttcgtc gcggtatcct
     2221 ttatcgcacc gcaacggaac tgggggcgac gaagatcgcg ctgggtcacc atcgtgacga
     2281 tatcctgcaa acgttgttct taaatatgtt ctacggcggc aagatgaaag gtatgcctcc
     2341 gaaactgatg agcgatgatg gcaaacatat tgtgattcgt ccgctggcct actgccgcga
     2401 gaaagatatt cagcgctttg ccgatgcaaa agcgttcccg attattccgt gcaacctgtg
     2461 cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg actgggataa
     2521 acgttatcca gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca
     2581 tctgtgcgat accaacctgt tcgatttcaa aggtattacc cacggttctg aagtggttaa
     2641 cgggggtgat ctggcgtttg atcgcgaaga gatccctcta caaccggcgg gttggcagcc
     2701 tgaagaagat gaaaatcagt tggatgagtt acggctgaat gtggttgaag tgaaataacc
     2761 cggatagcgc ccgatgcgca agcttaccgg gctactctta tggaggccgg ataagacgcg
     2821 gccagcgtcg catccggcaa tcccgaagaa gatgtttact cttgcaccct gcgattcaac
     2881 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa
     2941 ctgtttccat gctttatgag caacagcctg acgcaccgcg acatagacat gcgccggatg
     3001 cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca gcgcacctaa
     3061 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacataacg ttgccatttc
     3121 tgcttccagc ggcacaatgg aactattaac tggcggcgtt tgccagttaa gttttatctg
     3181 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggggcacaga aactgatcgc
     3241 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
     3301 ccacgccagc tcaaagttca ccaccagctc aagcgatttt atatccagac cacgcgcagc
     3361 aacatcagtc gcgaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc
     3421 gcgatcgcgt tgctccagat cgccgtgtaa cgacaatgca ctttgcccta cttcattcag
     3481 cgcgtcgcag acagactggc aatctttttt ggtattgcaa aacaccacgc aagaggatgg
     3541 ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttgctgg atgtctcata
     3601 aaattgttgt tcaataggtg gcagagcatc tgttgagtca atttcaatcg ctaaaggatc
     3661 gcgttgcact cttccgctga ttgcggcgat ggcttccggc caggttgccg aaaacagaag
     3721 cgtctgtcga gatgctggcg caaaacggat gacatcatca atggcatcgc taaatcccat
     3781 atccagcatg cggtcggcct catccatcac cagcgtattc aacgcatcca gtgataccgt
     3841 gcctttttgc agatgatcca gcatacggcc cggcgttgcc acgataatat gcggcgcatg
     3901 ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca aaattttggt
     3961 atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg ccagttcacg
     4021 cgtaggacac agcactaaag cctgggtttg aaatagcgac gcatcaattt gctgtaacaa
     4081 gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc gaacatcttt
     4141 tccggcaagg atcgtcggaa gcgcggcggc ctgcaccggc gtcatggtta aataacccaa
     4201 ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa aagcggacac
     4261 aatttattct cgtggtcatc gacgcaaagt cagcaggcgc gtatcctcgc agatctacgc
     4321 tcacgatgcg acaatttaat cggttcttcg tcgggtggtg ggtcaggcat gggttgcggg
     4381 cgagggatcg gatcgggcac tggaacagga tcgccaggaa tcggttcagg gacaggaatt
     4441 tgcaaataaa taagtgtcgt catatttccc tccggtcatt gggtggactc ttaaagggta
     4501 gacgctgaca aataacaggc aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc
     4561 aattgtgcta tgcagtaatt caaaaaagga agtaagacaa tatggagcgc aacgcccatc
     4621 gcttgacgtt gcattcacct gcaagagaga tattgccctg aatgggtaga gagtttattg
     4681 acttcgctca aactttgcgg cgtttttgta tacagacagc cgggaaaatt gcttttgtta
     4741 caaccattta ctacgatgca accataaagc aacaccacca ataagaacaa ctaacagaat
     4801 acaaaaaatt gaaaatccga attgccaccc gccgcctggg atcccaccaa ggttgacgcc
     4861 aaataaccct gtcagaaagg tactgggtaa aaagaccatt gccatcaacg acattgtata
     4921 ggtacgacgc gctaaatttt cctgcatcac ctgagcgatt tcatcagcca tcacgccagt
     4981 ccgtgctata caggcgtcga tttcgtcaag gccgcgccca aggcgatcgg caatatcctg
     5041 catacgacgg cgttggtcat cgctcatcca cggcaaacgt tcactggcaa gacgagcata
     5101 aacatcacgt tgcggtgcca tatagcgacg catcacaatt aattgtttgc gcagcagagc
     5161 caggaatcca cgcggtggaa tttgctgatc aaggagatta tcttcaaggt cgataatttt
     5221 atcgtgcagc tgctcgataa attcgctgga atgatcggtc aacgcatcac acacatccac
     5281 cagccatccc ccgcaatcgg tcggaccggt gccttcttcc agatcgctta ccacatcgtc
     5341 cagcgccagc actttgcgtt gtcgggtcga aacaattaac cgcccgtcca tatatacacg
     5401 catggcgacc agttgatcgg ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat
     5461 cagcgtacct tcaccgagac ggctgactcg gggccgtgtg ctctcgcccg ccagcgcatc
     5521 acgtacgtta ttgggaagca gcggtgttgt cgccagccat tgggcgctat catggtgtac
     5581 ataattaagg tggagccagc aggggtgcgc ttcatcaatc acatctgtat tttccagcgg
     5641 tttaacgccg cctctaccat ccagcatcca ggcaaatact gcatccggga cattaacgtc
     5701 cgctccctta atcgcttcca cagtgcctcc atcatcaacg cattattttg tagtctagcc
     5761 ttctggccct gttacgcaac atctcatcac cccattaccc tgaaatgatt aataaaattc
     5821 tgtctaaatt gaataaaaaa agcaaaatgc ttttccatat acaaaccgtg tgaagtgtta
     5881 aatagcgtct atcattatca gaattatctg atcatatgac gtggcttttt tgcgatcgga
     5941 tagcaacaaa aattgataaa aataacggga tctcaatgat tacgcacaac ttcaataccc
     6001 tggacttact caccagtcct gtctggatcg tttcgccctt tgaggaacag ttaatttatg
     6061 ccaacagcgc ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta cgaaccggtc
     6121 cctattccgt ctcctcacaa aaagaactgc cgaaatacct ctccgatctg caaaaccaac
     6181 acgatattat cgaaatcctc accgttcagc gcaatgaaga ggaaacagca ttgagctgtc
     6241 ggcttgtttt gcgaaagctg acagaagcag aaccggtgat tattttcgaa ggtatcgaag
     6301 caccggcaac gctgggttta aaagccagtc gctcggcaaa ttatcagcgc aaaaaacaag
     6361 gtttttatgc gcgctttttt ctgactaact ctgcaccaat gttgttgatt gacccctcac
     6421 gagatggcca aatcgtcgat gctaacctcg ccgcgctcaa tttctatggc tataaccatg
     6481 aaacgatgtg ccagaaacat acctgggaaa taaatatgct cgggcgacgc gtcatgccta
     6541 tcatgcatga aatttcgcat ttacccggtg gccataagcc tttgaatttt gttcataaac
     6601 tggcggatgg ttcgactcgt catgtgcaga cctatgccgg tccaattgaa atttatggcg
     6661 acaagctcat gttatgtatt gtgcatgata ttactgagca aaaacggctg gaggagcagc
     6721 tggaacatgc tgcccaccat gatgcgatga ccggattact gaatcggcga cagttttatc
     6781 acattaccga gccaggccaa atgcagcacc tcgccatcgc tcaggattac agcttgttgc
     6841 tcatcgacac cgatcgtttt aaacacatta acgatctcta tgggcattct aaaggtgatg
     6901 aggtgttatg cgcccttgcc cgcactctcg aaagttgcgc tcgcaaaggc gatttggtgt
     6961 ttcgttgggg aggcgaagag tttgtcttat tgctaccaag aaccccactg gataccgcgc
     7021 tttcgctggc tgaaactatc cgcgtaagcg tggcaaaagt gagtatttcg ggcttaccac
     7081 gctttactgt cagcattggt gtggcgcacc acgaaggaaa tgaaagcatc gatgaactgt
     7141 ttaaacgcgt tgatgatgct ttgtatcgag cgaaaaatga tggacgcaac cgcgtgctgg
     7201 cggcataagc cacggatgcg tctcgtgatc aacgactgcg cttagcgtgg cgctcccagt
     7261 tttcttgctt cgcctgcgct gttttacgta gtgcgacgta acacgccccg ctgccgccat
     7321 gatgcggtag cgcagtgcaa tatgcctgaa catcatcaaa ttcggtcagc cagcgcgcca
     7381 catagctgcg gacaatattg gcatgcgatt tatcatcccg tcctttgcca tgaataatca
     7441 gcacgttacg caaaccatcc gccagggctt gttgaatgaa actgaacacc attttgcggc
     7501 actcttccac cggctggcgc aaaagattca ggctcgcctg ttgcggatat ttaccactgc
     7561 gcagcttatc cagcacccca tgttgcaacc cttcccgccg aaactccagc ggctgagtta
     7621 gcgggatgat gtcgagaaat ccggtggtga ggaaattatc aagctgcagc gtgtcgatac
     7681 gctgcggcgc acgttggtta cgcgttggat gccagtggac atctgtagca cgtttcagcg
     7741 gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc atgtaacctc
     7801 ctatgcacct tcacacgtcg tgattcagac gggttaattg ttcatactta ctgcatcgtt
     7861 cccttcacag attgaaacaa tactctactt catacctgtg agtatctgcg caggcggggc
     7921 attttatact agcctgttag cagtatcgaa cagtttagcc cgagtttaat tcagcacagg
     7981 ctccgatcag gatgatttat tcatagcagg aaccaacaaa aatgcgataa ataacagacc
     8041 actgattagc agaaaggtat ccgaaaatgc catagtttgc gcctccctca gcatcaactg
     8101 actcaataat ttggttgaag ccagtatttc cgatgtctgg tcacttccac tgcgattaaa
     8161 aaacaaagca cttctggaaa tgaagtcatt caccgtatgg ggaacagaaa ccattttttc
     8221 acccattctc gaaaaatgaa aattagttcg attattgaga atagagccac acagggcaat
     8281 accaatcgcg cccccaagat tacgtgttaa attaaataca ccagaggcta acttcagccg
     8341 ttcctttgga attcccccca gcgttaatgt tacgatgggg gccatcgcaa actgctgcga
     8401 aatccctcta attgcctgtg gaaacagtaa ctcctgccat ccccattcgt gggttatagg
     8461 ggtaaaaaga tacattgaaa acacaaatcc acccagcccc gccatcaaca gccatcgcag
     8521 atttattttt ttacttaacc agaagtaaaa gggaacagaa aataactgga aaatacctgt
     8581 tgtgcaaacg gcaaaaccta tctcttcggc atttaatccg cgaacctgcc ccaaaaacac
     8641 aagtattaaa tatacagtag agaatatcac aacccctcca gaaaaagaaa aaaaacatcc
     8701 cagcgtgaaa tttttatcct taaaagcatg aagatccatt atcggattgg atattgtaag
     8761 tgtacgcgca gcaaataaaa taaaactcac caatgccaga actgatgtca gcaaaatagt
     8821 attatcatcc agccatcccc agcgcgctcc ttcttcaagg gtatattcca gacatcccag
     8881 tgtcatggcc aacaggataa tactcggata atcagcaact ttcagtaaag agagatcagg
     8941 tttatcaaaa ttaaccagaa aaggaatact caaaactaaa tatatgccag gtaatatatt
     9001 gatataaaat agccatcgcc agtcgaggtt atcagtgatc cagcccccca gagttggccc
     9061 tagcgtcggt gacagtgagg ccagcgcact gactaccgca gcagccgacc ccaactcttt
     9121 accctggtaa taaataaacg ccgtcgtgaa aactaaaggg atcatggatg cgccagccgc
     9181 tccctgtaaa gcacgaaaca aaatcatgat ctgaatattc catgcaagtc cacacgcgat
     9241 actcattaaa gtaaatattc ccgcggatag agtaaaaagc caacgagtgg aaaatacccg
     9301 tgacagccac ccagagagcg gaataacaat aatctcagca attaaatagc tggtctgaag
     9361 ccaggccatt tcgtctttac ctgcggacag accaccacct atttcattta gggatgacga
     9421 tacaatttga atatcaagct gagcaataaa taatcccata cacatactgg cgaaagcaat
     9481 atgtttacgc atagacatca tctctcatcg acactgacgg tgacggacag tcccgggcga
     9541 agaagttgct ttaattcctt tgcgtcgtca aatgcaatcc gtaccgggac acgctgaaca
     9601 attttggtga aattgccagt ggcattttct atcggaatca agctgaaact ggctcctgtc
     9661 gctggggaaa ggctttcaat gtgaccgtga aacacttccc ctttgagaat atcagcacgg
     9721 atctcagcct tcattcctgg cttcattccc gctatttggt tttctttata atttgcatca
     9781 acccacaact cactgactgg tacaagtgaa actaacgatg tgccgccttc cacccaactg
     9841 cctgtatggg cgctgcggtt tgcgacaatg ccatcaatcg gcgcacgaac aagggtatat
     9901 tccagatcca gtagtgcctg agtcaaagcg gcctgaactt cttcgatact cgcacgtacg
     9961 ttttcttcct gagcagataa aactgccagc cgttgcttct ccaccaggaa atcgttcgcg
    10021 gctttgcgct cctttgctgc gacctgctgg tagtcaaacc tggcgttatc aataatttgc
    10081 tgcgatattg ccgctgattg tacgagtttt tcataacgtt cagtatctcg caggcgcttt
    10141 tgctcctcat gttttactgc ctgccatgtt tcactggcac tttgaattat cgactgctgc
    10201 atcgtaatag tggcttgaat gctttcgaga ttggcttttc tactttttat ttttgcctca
    10261 agcctggcga catttgcacg ataatcccga tcatcgagac gcaggataat atcgcctttt
    10321 tttacgctct gattatcacg cacctctata gcagaaatat aaccagaaac tttacttgcc
    10381 accgtggtaa tattaccacc aatgtaagca tcatccgtcg tctgtataaa tcttcctttg
    10441 cttacccacc agataaaaaa gacaacgcca gccaaaataa taccgatcgc tactacccca
    10501 ataagctgtt ttttagaaat tatcatcact ctttacaccg tatatatccg gaaacttgca
    10561 ttcgccatat ttaaaattta acatggctta tttgatgatt tctgacaaaa tacctactgt
    10621 aaatgacaat cacactgcaa cccaaagcat caccagggca tcacattatc ggactggatt
    10681 cggaacatct gaatggaggc acagttccct ggcataaaca tctctatgcc cagttgcttt
    10741 acccggctga aggagtcgtt cgtgtatggg ctggagaaag cgtctggcta gtgcatgcca
    10801 gcagcgcgct ttggctgccc ccacaaatgc cgcataagtt tgttgcgaca ggcaacgtac
    10861 ttttaaaaac cgtactggtt tcagaagcgg aaagtgaaac gctgggaaaa gtttgcttca
    10921 tgacgggtat ttcacccctg ttaagagaat tactaattgc aattaaccaa cttccaccgt
    10981 cgcaaagtac cacagataaa caacagttac gtttttcagc cctcgagaca ctgatacttc
    11041 aggaaataaa aatgggggtg aaaatgtcac ttgaactccc ttggccaaat gatgagcggc
    11101 tacagcagct ttgtgagaat ttgctaaaca accaagggta tctgccaaca ctggataatc
    11161 tagcggataa aattaacgtc agtagccgta cactcatgcg cttatttgtt aaagagacgg
    11221 gattaacatt tcgccactgg gttcagcaaa tgcacgttat ttctgcagtg acgcttcttg
    11281 atgatggata ttcgttgacc aaaattgccc atcgtctggg ctatgcctca gctgaatcgt
    11341 tcggcaatat gtttaagcgt cgaacgggct actcgcccgg taaatttact cgtcgattaa
    11401 cgatgcatga ttatgcaatc acaaggcaaa tgatttaact tatattttcg tgaaatctgt
    11461 cactgaagaa aattggcaac ggatgggtaa aaccgttata acactgtcac cggtcaccag
    11521 gaccccaggc cggatttaag acgaggatgc actgctgtgt gtactgtaga gtctggcgga
    11581 tgtcgacagg ctctattttt ttattcattt tttagctctg taaataacca cattctcgcc
    11641 gggattgggg aatgagtgac gagatcagtg gtgtctggcg gctatcacgc cacagaatca
    11701 aataacagat agcctacggt aaaatttcgc tgactggaat tatcccaccc tcgatccatg
    11761 tgatggcttg ccctcatttc ttcaggcaga aaaatcaaaa aatcgcttta tgctatcaga
    11821 ctgcgacaaa ccagacgcga aactgggtac ttactattcg ttagtcttgc cctatccgct
    11881 tatctttttg gtggtatggc tgctgatgtt gctggcgtgg tatcttgttg gtctgccgat
    11941 aggtccgggg atttacccac gtttgtctta agagagaacg gatgctgaga ttacttgaag
    12001 aaaaaattgc cacgccactg ggtccactat gggtgatttg cgatgagcaa tttcgcctgc
    12061 gggcggttga atgggaagag tacagcgaac gcatggtgca gctgctggac atccattatc
    12121 gcaaagaagg ctatgagcgc atttctgccg ccaacccagg tggtttaagc gacaagcttc
    12181 gtgaatattt tgccggtaat cttagcatta ttgatacgct tcccactgcc acggggggga
    12241 cgccatttca gcgcgaagtc tggaaaacac tacgcactat cccctgcggg caggtaatgc
    12301 attacggtca actggccgaa caattgggtc gccctggcgc ggcgcgtgcc gttggtgcgg
    12361 ctaacggatc gaatcccatc agcattgttg taccttgcca tcgggttatt ggccgaaacg
    12421 gcaccatgac cggatatgca ggcggagttc agcgaaaaga gtggttattg cgccacgaag
    12481 gttatctttt gctgtaaaca ttaaacaatt tgtgccagct tgttcacact tttatgtaaa
    12541 gttaccctta acaacttaag ggttttcaaa tagatagaca tatatttaca tctaatatcg
    12601 gaattctctg ctgttaaggt ttgcttagac ttacttgctc cctaaaaaga tgttaaaatt
    12661 gacaaatatc aattacggct tgagcagacc tatgatcccg gaaaagcgaa ttatacggcg
    12721 cattcagtct ggcggttgtg ctatccattg ccaggattgc agcatcagcc agctttgcat
    12781 cccgttcaca ctcaacgaac atgagcttga tcagcttgat aatatcattg agcggaagaa
    12841 gcctattcag aaaggccaga cgctgtttaa ggctggtgat gaacttaaat cgctttatgc
    12901 catccgctcc ggtacgatta aaagttatac catcactgag caaggcgacg agcaaatcac
    12961 tggtttccat ttagcaggcg acctggtggg atttgacgcc atcggcagcg gccatcaccc
    13021 gagcttcgcg caggcgctgg aaacctcgat ggtatgtgaa atcccgttcg aaacgctgga
    13081 cgatttgtcc ggtaaaatgc cgaatctgcg tcagcagatg atgcgtctga tgagcggtga
    13141 aatcaaaggc gatcaggaca tgatcctgct gttgtcgaag aaaaatgccg aggaacgtct
    13201 ggctgcattc atctacaacc tgtcccgtcg ttttgcccaa cgcggcttct cccctcgtga
    13261 attccgcctg acgatgactc gtggcgatat tggtaactat ctgggcctga cggtagaaac
    13321 catcagccgt ctgctgggtc gcttccagaa aagcggtatg ctggcagtca aaggtaaata
    13381 catcaccatc gaaaataacg atgcgctggc ccagcttgct ggtcatacgc gtaacgttgc
    13441 ctgatttttc cgcataactc actatccttc tgtcatatca ttaaattttt ctgatttatt
    13501 gatctggcag aaggttcatc actgtttcat tcaccagata tgggttaatc ttttaattac
    13561 aaactgcgtt gacagttgtt gtaaggagac cctgtatggc tatgtatcag aacatgctcg
    13621 ttgttatcga tcctaaccag gacgaccaac cagcattgcg gcgagctgtt tatttacatc
    13681 aacggattgg tggcaaaatt aaagcctttt tgccgatcta tgacttctca tacgaaatga
    13741 ccaccctgct ctccccggac gaacgtaccg ctatgcgtca gggcgtcatc agccagcgta
    13801 cagcctggat ccacgagcag gcaaaatatt atctcaatgc tggcgttccc attgaaatta
    13861 aagtggtctg gcataaccgt cctttcgaag ccatcattca ggaagtgatc agcggcgggc
    13921 acgatttggt gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg atttttacgc
    13981 caacggactg gcatctgtta cgcaaatgcc caagccccgt gtggatggtg aaagaccagc
    14041 cgtggccgga agggggtaag gcgctggtgg cggtgaatct cgccagtgaa gagccgtatc
    14101 ataatgcgct caatgaaaaa ctggtcaaag agacgatcga actggcggaa caagtcaacc
    14161 ataccgaagt tcatctggtt ggcgcttatc cggtaacgcc aattaatatc gcgattgaac
    14221 tgccggaatt tgatccgagc gtttataacg atgccattcg tgggcaacat ttgctggcaa
    14281 tgaaagccct gcggcagaaa ttcggcatta atgaaaacat gacgcacgta gaaaaaggtc
    14341 tgccagaaga ggtgattcct gatttggcgg agcacttaca ggcggggatt gtggttctgg
    14401 gcacggtcgg acgcaccggt atttcagcag cattccttgg taacacggcg gaacaggtga
    14461 ttgatcatct tcgctgcgac ctgctggtca ttaaacctga ccagtatcag acacccgttg
    14521 aactggatga cgaagaagac gattaacgac tcttcaataa ctgacaaggc ccgagttatc
    14581 gggcctattt tttacttact gctcttcgcc gccaaggaaa taaattccca acgggattgc
    14641 cagcaacaca gtaaagacca ggctataaac aaaaatcatc gatgactgaa tcacatacat
    14701 tgatgtcgtc cactcagaca gcggaatatt gtactgttct atcacgccgc cgatggtcgc
    14761 gcgagtcaca accgacgccg caataatgaa aaccgccaac aaaccaaaga gaaatttctt
    14821 ccctttcgct gacttcagtt tcgccataat catgatgtaa tccttgtgca gacaaatttc
    14881 ttgttaccta aacgtaacaa taccatgaca tttcaccgtt gtctgctctg aaaacctcac
    14941 ctcttagctg gatttacaat caacgcattg attagttagc taatgttgct acttccttcc
    15001 ctgactattg atgatctgaa tgaattgtcg cggtaaaata gccccgccag gggtttccgg
    15061 catgacgaca attatctgga ggagcctgat aaaacaatga tcgctgaact gtttacaaat
    15121 aatgcgctta atctggtcat tattttcggt agctgcgcag cattgattct gatgagcttt
    15181 tggtttcgcc gcggaaatcg taaaagaaaa ggatttttat tccatgcggt gcaattttta
    15241 atctacacca taattatcag tgctgttggt agcatcatta attatgttat agaaaactat
    15301 aaactcaaat ttatcacccc aggcgttatc gattttatct gtacgtccct gattgcggtt
    15361 attctgacga ttaagctatt tctgctgata aatcagtttg aaaaacagca gattaaaaaa
    15421 ggtcgcgata tcaccagtgc gcggattatg tcgcgtatca tcaaaatcac cattattgtg
    15481 gtgcttgttc tgctttatgg tgaacatttc ggtatgagcc tttctggctt gttgaccttt
    15541 ggtggtattg gtggtctggc tgtcggtatg gccggtaaag atattttgag taacttcttt
    15601 tccgggatta tgctctattt cgaccgtcct tttagtattg gcgactggat ccgttcaccg
    15661 gacagaaata tcgaaggtac agtagcggaa attggctggc gaattaccaa aattacgact
    15721 tttgataatc gtccgttgta cgtaccgaac tcgctgtttt cgtcgatcag cgtagaaaac
    15781 ccaggacgaa tgaccaaccg gcgcattacc acgaccattg gtttacgtta tgaggatgcg
    15841 gcaaaagtgg gcgttattgt cgaagccgta cgtgagatgc tgaaaaatca cccggccatc
    15901 gaccagcgac aaaccttact ggtttatttc aaccagtttg ctgactcttc attgaatatt
    15961 atggtttatt gctttaccaa aaccacggta tgggctgaat ggcttgctgc acagcaagac
    16021 gtttatttga agattatcga tattgtacag tcacacggcg cggactttgc cttccccagc
    16081 cagacgctgt atatggataa cattacaccg ccggaccagg gtcgctaaat cagacatcac
    16141 tggcgttatc cacaagggta acgccagttc ccatcacatc aatgcttcac aatatacata
    16201 gtccgactgt acgccacatc ttccgggtta ttaatgggat aacctttcag ccacggcttg
    16261 attaatcgtc cattggtata ttgataaatt ggtgcaatcg gtgcttgctc catgaggatt
    16321 ttttctgccg tattgtaatc ggcattacgc gctttaacgg tattttccgt tgatgcctgg
    16381 gccagtactt tgtcgtatac cggattgtta aagcgtgaaa tatttcctga atgcgttgac
    16441 gttaataatg tcaggaaagt ggagggttca ttataatccc ctacccacga ggctcggata
    16501 acatcaaaat tgccggtgtt acggctatcg atataggttt tccattcctg attttgcaat
    16561 ttaacatcta cgccaaggtt ctttttccac attgatgcta cagcaatcgc aattttttga
    16621 tggttttctg aagtgttata caaaagcgtc agcttcagcg gtttttgcgg accataacca
    16681 gctgcgctca acaaagtttt tgcctgcgca ttcagttctt cctgactcat ttgttcaaac
    16741 ggcgaaggtt ccggcgtaaa tcctgcagta acatctggtg taaaatgcca cgctggcttt
    16801 tcgcccgttc ccaacacttt ttcggtcatc aggcggcgat ctatcgtcat acttaatgcc
    16861 aggcgaacac gctgatcggc cgtcggccct ttttgcgtgt taaacgcata ataataggtc
    16921 ccgagctgtg gcggcgtata aacctgcccc ggaatatcct tcaacagctt ctgatacata
    16981 tttttcggga aggattcggt gatatcaata tcccccgcga gataacgctt agtggctgcg
    17041 gattcctgat taattggcag gaaggtcact ttttgcagca ccgttttggc gttatcccaa
    17101 taatgggtat tcggtaccac gaccagtttt tcattgacta cgcgctcttt aagaacataa
    17161 gcgccattac cgatcagatt tccgggtttc gtccactctt tactgctttc tacgttggct
    17221 ttttgcaccg ggaagaaggc aaagttagcg gttaaattca caaaccacgg caacggttta
    17281 tcaagctgga ttttcaaagt ttgggcatca actgcggtga cgccaagctg gtcaggcgcg
    17341 gctttaccat caataatcgc ctgtgcgttg ttgattcccg ccagcgcggc aaaccatgca
    17401 aatggcgaca atgtttttgg gtccaccaga cgttgccagc tgtagacaaa atcttgcgcc
    17461 gttaccggtg tgccatccgc ccattttgcg ttatcgcgca gggtaaaagt ccagatacgg
    17521 ttgtcattac ttttccactg agtcgcaacg ccggggacaa tctccccttt ctcgttctga
    17581 ttcaccagtc cttcaaacag atcgcgaatg acctgaatct ctggcaggcc cacggcttta
    17641 gcgggatcca gcgacgcagg ctcatcttta atgtggcgca ccagctcctg cttctctgcc
    17701 agtactgtgc cgctcggaac ttcagcagca tacgaaagag aaatgctgct gaccaacagc
    17761 gcacaacacg tgactgaaac agagtgcttc ataagtttcc ctctaaccga cattaaaatt
    17821 gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca acttccatca aaaaagtgac
    17881 aaaggaagca tcaaatgttt taagtatcaa tatatcaaat cagatttagt gtaaataata
    17941 gtctccaaaa tgcggtaaag cacatagaac tccgaacact acgttcgggc ttcgcacgtt
    18001 caaacccgtt ttcggtatca taatcagcat ctgtcgccat tgtacgaaga ataaaaaata
    18061 accaaaacgt tgaaaggaac tgagaatacg ttaagagaag aattaaatta atgtatcagc
    18121 aagtagtgct aaacagcacc tttatacttc agcctatcta taaataatga gaatgccgag
    18181 ccagcatgtc gacggtgtgg atagtacagg tgataaccgg gtaaatctgg tgtgaattta
    18241 tccaaaacac gtatcagttt tttttcttta atagcccgct caacctgatc ataaggtaga
    18301 tacgccaatc catgcccatc aattgcagca tcaatgatca ggtctatcgt attcagtaaa
    18361 agctgacctt ccatgcgaac acgaacttca cgtccaccgc gtattaatct ccagcgattt
    18421 gctgtacccg atgtgggaag atacaaatta attgcctgat gatctattaa ttgtgacact
    18481 gaggttggag cacttcgacg agaaaaataa tccggtgagc caacaatagc cattggtata
    18541 tctggcccga ttcgaatggc gatcatatct ttatccatct ccccacccag acggacgcct
    18601 gcatcaaaac gttcagaaac gacatcggtc aaaccataat caatggtaag ctggatatca
    18661 atttcaggat gcgatttcag gaatgtgcgc atagctggta acaatatcgt ttttgctgca
    18721 tgttctacag tagtaatacg tattgtcccg gatgggcggt tctgcagatc gctcagggat
    18781 gtcagggcag aatctatgtc atgcaacata gggccaagaa cagacaaaag atgctcgccc
    18841 gcttcagtgg gtacaacgct gcgcgtggtt cgtgtcagaa gccgcaatcc caatcgttct
    18901 tctatacggc gcactatctg gcttaaagct gactgcgcca tgctcaggcg ggctgctgcg
    18961 cgagtgaagc tacgctcctc tgcaacgacg acaaacgcca tcagatcagc gatttcttca
    19021 cgcttcattc agtatctcca tacaatctat taatcattta aatgataggt ctaatcatga
    19081 ttttgggtct aatcaactta atgatcaata tctatagtga tatcaccaac cgtcaaaacg
    19141 aaccgacaac tccatgagga acggaatgat gaataataaa gtcagcttca ctaacagcaa
    19201 taatccaacc atctctttgt ctgcagtgat ctatttccct ccaaaatttg atgaaacccg
    19261 tcagtatccg gcaattgtgg tatctcaccc aggtgggggc gttaaagagc aaacggctgg
    19321 aacctatgcc gaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
    19381 tcaaggtgaa agcggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
    19441 cgtcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata gccgaattgg
    19501 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
    19561 catcaaggcc atcggtactg ttagtgcggt caatattggt tcgatgttcc gtaatggttg
    19621 ggaaaacaat gtgaaatcta tagatgccct accatacgtt gacgcgggtt caaacgcccg
    19681 aaccagcgat attagcagca gagaatacgc cactatgcca ctggcaccaa tgaaggagtc
    19741 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccataccc ctcgcgcgca
    19801 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctatga
    19861 tgcttaccat atggcggaag tgtacctgac tcagccgata cagattgtgg cgggcagcca
    19921 ggcagggagc aaatggatga gtgacgacct gtacgatcga gcatcaagcc aggataaacg
    19981 ctatcacatt gttgaaggcg caaaccatat ggatctttat gacggcaaag tctatgtggc
    20041 tgaagcaatt tcagtattag ctccgttctt tgaggaaact ctgtaatgaa acatattcaa
    20101 atccgtaata gtgatatggc ctggcatatt gctgccaata atctgggaaa agtttcatga
    20161 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
    20221 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
    20281 cctatcccac taatagcaag attattatgg gggatgtact gaatcatgca gcactgaagc
    20341 aggccatgca gggacaggat gttgtgtatg ccaaccttac gggggaagat ttagatattc
    20401 aggcaaattc agtgatcgct gcgatgaaag ccggtgatgt taaacgtctg atctttgttc
    20461 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
    20521 ttggtgaacc cctaaaacca ttccgcaggg ctgcagatgc aattgaggct tctggactgg
    20581 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
    20641 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
    20701 ttacagatat catcgataaa cctgagaaac acattggtga gaatatagga atcaatcaac
    20761 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagct accatcgtac
    20821 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
    20881 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
    20941 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
    21001 ataacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
    21061 aatacttcct gaaccagaga cgatcatgac cgtaactcgc ccacgcgccg aacgcggcgc
    21121 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
    21181 tccggcccct gcagccagtc acgaaagtgg tttgattctg gctggcactc acggtgatga
    21241 aaactcttcg atcgtcacgc tctcctgtgc tctgcggacg ttgacacctt ctttgcgccg
    21301 tcatcatgtg gtgctgtgtg tgaatcctga cggctgccag ttggggttaa gggccaatgc
    21361 taatggtgtg gatttaaaca gaaactttcc ggcggcaaac tggaaggaag gtgaaaccgt
    21421 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgtt ttactgacag gtgacaaacc
    21481 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacccgcctg
    21541 ggtggtctca ttccacgatc cgctggcctg tattgaagat cccagacaca gcgaattagg
    21601 tgaatggctg gcccaggcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
    21661 tggctctttc ggcagttggt gtgcggatct caacctgcat tgcatcaccg cagagtttcc
    21721 acccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
    21781 ctggcatcct aaagatgcaa ttcgcccgtt gtgaactgaa gcgccggttc cacatctacc
    21841 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
    21901 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactaaa accttgtgca
    21961 cgtgcttcag tcgccagcgc cagcgcttcc gtcaggcccc ccgttttatc gagcttaatg
    22021 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
    22081 tcagcacaaa tcggtaacgg atgaataaaa ttctccaacg ccgcatcgtc ctgcgcagga
    22141 agcggttgtt caagcattgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
    22201 ccttctgcac gccaggattc atttgcatca acgatcagcg tcgcatcggg cacagcggtg
    22261 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
    22321 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
    22381 accgtctgtg cagtgataac tgtcccggga agcgttatgc cgatcaaatc agccagcgat
    22441 tcctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
    22501 ccggcaggga ggattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
    22561 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
    22621 cattcaccgg tgcctttaat accttcttct tccagttcaa ccaccaccac gcgcgcttca
    22681 ctgcgacttc cccgagcaat cacaaacggg gtatgtaagg gccagccttc ctcgaatacc
    22741 ttaacggttc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
    22801 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
    22861 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
    22921 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
    22981 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
    23041 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac agagatcgac
    23101 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
    23161 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
    23221 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
    23281 gttattgatg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
    23341 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
    23401 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccgtattccc
    23461 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
    23521 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacgactg gtgatttcgt
    23581 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
    23641 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
    23701 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
    23761 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
    23821 cggttaacgg catgatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
    23881 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccaaat
    23941 tcttctgcgt agcgcaaatc acccttacat cgacatgcac ttcatgatct tcaccaactc
    24001 gacggaaagt accatcattg aggaaacgca gtaatttcgc ctgcatcctt ggcgacatct
    24061 cgcctatctc atctaacagc accgaaccac cgttcgcctg ctcaaagaac ccttttttac
    24121 cttccggagc atgaccaaac agttcactct cgaccgcatc ttccggtata gacgcgcagt
    24181 tcagcgccag gtaaggtttg cccgctcgtg ggcttgcctg atggcaggcg taggcaaaga
    24241 gatctttacc tgtaccggtg tcacccgtaa tcagcaacgg cgcgcttagc atcgccagtt
    24301 tctgcgcctg ttcgacaaca tgcttcattt tcgggctgac ggcgacaatt tgactgaagg
    24361 cactgacgtc ctgtgcagcg acattctgca actgacggcc catacgaatc gtcgagcgca
    24421 acatcaccac cgcaccggtc agaacatgtt gatcattttc atcctgaaga taaacaggcg
    24481 taatctccat caggaaattc tgcccattaa taacaacatg ctcgttatgc gagtcttgcg
    24541 gttcgctttc cagccagcgt aaaaaattaa agccgttaat taattgcgcg gcggtatggt
    24601 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
    24661 ctttgctttt catatcaaca gaaagcacag gctcaggcaa cgcctccagt aacgcgctca
    24721 acgccagatg ctcacgttcg gaaggcatcc acggaacagt gcgcacatcg gtaacacctg
    24781 caatacggcg tatttcggcc atcagactgc tgaaactttc aaattccagt tcggcaaaat
    24841 tgaggtagat tcgcccaatg gggtcaatct caataccgcg taaatcaatg cctctcagca
    24901 ccagtagatc gagtaattcg cgggtcagac caagtcggtc ttcacaaaag acttccagac
    24961 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
    25021 agcaggaagg tttctgtcaa caatgattga cacaaagacg gaaaaacggg cacttcggcg
    25081 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccctttt
    25141 gcagcgtctc tttcacctga ccgataagct gacgacggaa atccccgagg cgaggtttgt
    25201 catcttcaat ccacggcagc gggcggcaaa gttccatagc tttaatcccc agtcgtgccg
    25261 tcagaagtcc agcaccaatc ccctgagctg cccgggtaga caaacgcgca gcgagatctt
    25321 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggcg aaagcgatat
    25381 tcagcaatac cagcttaaac aggcgcaaac ggctgtaata tcccagttca atgccataca
    25441 gcgtggcgat gcgattgatc aaacgcagat tgcgccaggc gataaaagcc atatccacca
    25501 gcgccagtgg gctgaccgca atcatcaatg ttgattccgc tgccgaacgg ctgatttcgc
    25561 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaaactg accacttcac
    25621 ggtcgttttg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgtgactgat
    25681 cgatgcccgc ctgctgtgcc agtttttcgc aaaatgcgcg gcctttaccc gtgccgtgac
    25741 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
    25801 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
    25861 atccacccag cgccacccag tcctgggttt gccaggcgtt cattgtccac tgtacaccct
    25921 gcccgacaac gcttgcgcca aacagagcca acccgcccat caccattttg cgccacaggc
    25981 tgcgtttcgg acgtaacgct gcgtccatca ccgcttcaac ttgcccctct tcttcctgcg
    26041 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
    26101 ccctgaattt tggattctga tcgacctcca gtggaccgtc gaaatcaata cgtggtttta
    26161 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcagcat caaggcgaat
    26221 atgcggtagc ggtttgtcga catccatcac ctgcggacga aacgcctcaa actggaagcc
    26281 ttgcttatcc cagaacgcct gaccaggcaa acgtgcgggg acttcgccag gataaacagt
    26341 gagcggtgcg ccatcactaa gtcgattacc gcgcagcgcc gggattttct caccgttaac
    26401 atcaataatg ccgctggtgg tcgcctgaac tgacgccagc cccaggcagt ccatgctgat
    26461 cccttcgaac gctgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
    26521 attagcgtgc tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc
    26581 gataaccggc gaaaacaaac gccggaacag ggtacgctgc ccgtagtgga aactttgcat
    26641 cagctgcgtc agcgccagac gcatatcatt aaatgcctgt ggtccactgt tgagcggttg
    26701 caggcagtcc accagcacaa tctggcggtc aaaacgcagg aagtgatttt tatagaatcc
    26761 tttcaccacc ttctcgcagt aataattaaa acgctcgcgc aacattccgg cgttggtgtg
    26821 tttatcggcc tgcgccagtt tagactcgcc ccaggcatcg acatctggcc atgggaagaa
    26881 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
    26941 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc
    27001 cagccggttt tcgtcggcag gcgctagcgg gtccagccct tcgcacatca ttcgccattt
    27061 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
    27121 atagtcctgc gccagcatcg gcaggtcgag caaccattcg ccggggtaat ccacaatctc
    27181 caaatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttatagcg
    27241 tagcgccagg cgaatttcgc tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc
    27301 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
    27361 aatgcgtttt acgcccagca ggcgttcttc acgcaccgca cttaatagcg gcaaacgtgc
    27421 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
    27481 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
    27541 attaagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
    27601 aatcattcgt aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg
    27661 tcatcgacgg gcgaactcca taagatgtgc aacattcacg ttcggacgga gttcctgacc
    27721 tatgtcccct actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc
    27781 acgcgtgctg aataagcaaa ccaatatctc cccggaagcg cgcgaaaaag tgttacgggc
    27841 cattgaagaa ttacagtatc aaccaaacaa gctggcacgc gcgctgacct cttcgggttt
    27901 tgatgccatt atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt
    27961 ctcggaagtt ttacatgcca tcaccgccaa agctgaagaa gaaggtttcg acgtgatatt
    28021 gcagacgtcg cacaacctgg cagaagactt acaaaaatgc gaaagcaaaa ttaagcagaa
    28081 aatgattaaa ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact
    28141 cgataaatat gatattccgg tggtggtaat tggcaaagtt gaaggtcaat atgcccatgt
    28201 ttattctgtc gataccgata attttggcga cagcattgcg ttgaccgatg cgttaattga
    28261 aagtgggaat caaaatattg cctgcctgca tgctccgctt gatgtccatg tctcggttga
    28321 ccgcgttaat ggctataagc aaagtctggc tgcgcataat attgcagtgc gtgatgaatg
    28381 gattgttgac ggcggttata cccatgaaac agccttgcaa gccgcacggc aattattaag
    28441 ccagtcgccg ttgccagaag ccgtatttgc cactgacagc ctgaaattaa tgagcattta
    28501 tcgtgcggca gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggctatag
    28561 caatgaaacg ctgtcattta ttttaacgcc tgcaccgggc ggcatcgatg ttccgacgca
    28621 ggagttaggg caacaaagct gcgagttatt attccgctta attgccggaa aaccgtcacc
    28681 acaaaatatt accgttgcca cgcatatgtc gttgaaataa atgtatgccg gatttggcgc
    28741 tcgcgccgca tccggcaatt taacccatta tcagaacgag taatttacgc cgacacctgc
    28801 ataatggaat ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag
    28861 cgaaacggaa agaccattct ggaaatcata accgtaaaat aaccccacac ggttaaaatc
    28921 atggccttca cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat
    28981 gcgcgtatac ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc
    29041 ttgcgtggaa aactcaccgt tattgcggct atcatccata ttgaaaccgc gctcgagata
    29101 atagttcact cgcaaggcaa ccgtttcgtt gaaggtatat tgcagacccg tttccgtttc
    29161 gacacgggta tcagcataac cggtcgtatt cagatcgttg gcaaatttat acatcgacag
    29221 ccaaccgttg aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg caattttcca
    29281 gcgctgcata ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg
    29341 gaaaccacca gtcagaccga aactgaaatc atcgctttcg aggaactgat aatgtacctc
    29401 cagctccggg cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc
    29461 ctgataatag gccagagaaa ttctccacgg cccgttggcg gcattaaaat agacagaagg
    29521 ctccgccagc ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc
    29581 gccgatatta aagtgccagt cgttcttttc ctcggcctgt gcgcaggcca ttcccgcaca
    29641 catcaccagt gcggtacagg gtaataattt tttcattatt gttccttgta ttatcctggg
    29701 ggaatgcctt ccccctgtat ttagcgaatt gctatttccg tttctgcatc aaagaaatga
    29761 catttagtca tatcaaaatg aatagtgata ttttctcctg catgataatc atttaacgca
    29821 ccagcacgga ccaccagttc gtgcccccca accgtggtgt agagcataaa ttcagcaccg
    29881 gttaattctg ccacgctaat tttggcggaa atgttatttt cctcttgcgc gtccggatga
    29941 atatcttccg ggcgtattcc catcacaatg ggcttatgca aactttcctg tgttttcaga
    30001 accgctaatt tctcctcgga aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca
    30061 tcaatcgtgc cgcgaataaa attcatcgcc ggtgatccta taaatccggc aacaaacata
    30121 ttcgccggtt ggttataaac cgttttcggc gcacctactt gctgaacaat cccatccttc
    30181 ataatcacaa tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt cacgtagatc
    30241 atggtggtgt tcagtttctg atgcagcttg ctgatctctg cgcgcatttg cacgcgcagc
    30301 ttggcatcca ggttagagag cggttcatcc attaaaaaca cgcccgcttc gcgcacaatc
    30361 gcccgcccga gcgccactcg ctgacgttgc ccgccggaaa gcgcccccgg cttgcgtttc
    30421 aggtactcac gcaggccgag aatttgcgcc gcccagttca cccgctcatc aatcacctcg
    30481 ttggcgattt tttgcatctt caggccaaac gccatgttgt cgtagaccgt catatgcgga
    30541 tacagcgcgt agttcaggaa caccatcgcg atattgcgct ctttagccgg aacgtcattc
    30601 attcgtttgc cgtcgatcaa cagatcgccg ccgctgatct cctcaagccc ggcaatcatg
    30661 cgcagcgtag tcgatttacc gcagcctgac ggaccgacaa agacaatgaa ctctttatcg
    30721 gcaatttcca ggttgaagtc cttcaccaca tgcacctggt tatcgtagat tttttgaata
    30781 tgttgtaacg aaagctgagc catgttgatt cctttgctat acgttttgcc agaaggccga
    30841 taaccgcggc caggtcaggg agtccgttga gggcaacaat aattgcgccc cggttaagcc
    30901 cgcgccgatc cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc
    30961 atcttcaatg ccgatacatg cctgcggcgg cacgcccagt ccggcacagg cggcgagaaa
    31021 gatttccggg tccggtttcg agtttttaag ttgggaagcg tccgcgcaga aggtgaaaaa
    31081 ctcgcgcagc tccagcgctg ctaaaatcgt cggcgcattc agggagacag aagccagccc
    31141 tatagggatc tgctgtgcac ggagatctgc cagcaaattg cgaatgccgg gcagcacagc
    31201 attaaccgtc agttcgcgca gtgagtggac gtagagcaga tttttgcgat acgccagttg
    31261 cgccctctcc tgcgggttaa agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag
    31321 agactcatcg cggctgatcc cttttaggga ttcgttaaac tgcgcatcaa tgctgatgcc
    31381 aatttcagcg gcaatctgct gccacgcctt gaaatgcaga tgcgcggtat cggtgattac
    31441 accatccaga tcgaaaatta ccccttgcag tttcattcat cctcctgatg cgtggtagcg
    31501 gtcccattga agggcaaaat aaaatcaccc aaacagaaaa cagattcttc agccacagat
    31561 ataagctgcc cgttcaaacg cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc
    31621 gcgtcgagag tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga caactgttgc
    31681 cactgctcag gtaacgctgg attgagatgc aattcaccgt tacgcacgct caccccggca
    31741 aaaccctgaa tcgcccccag ccaaatagcg ccagttgcgg ctgcgtggat accgtcatca
    31801 cagctatgcg gatcagcacc aagatcgatt tcagtcccct cgcgccagaa ctgataactt
    31861 tgcgccaaca ggccacagcg tgcggcaaca atgccgtgga tcgctttact cagtgaagag
    31921 tcgtgaatgg tgcgcggttc ataaaattgc agattggcaa gacacgatgc cgctgagtac
    31981 tgctccggca gcatgtaatt gagcatcacc acatcagctt gtttgaggat ctgcatctcg
    32041 ttcacttctg cgcgtgaata atccagcaga atggtttgct tccccgccgc cgctttgtat
    32101 ttcgccagat taatcgctgg tttcgccata aacgaatcat cctgcggcaa aacgccgtca
    32161 ggctgaattt ctggcagccg cagttctttg aggaatattt cggcacgatg aataaacgcg
    32221 tcgtcgctac aaccaaactt acgagcgata ttcagcgcct gctggacgtt gtaatacgcc
    32281 atatagctgg tgaaggcgtt attattgaca tgttcggtat attcgtctgg cccaataaca
    32341 ttgtgaattt ccagacgatc gtttacctgt acagcgcggc taatccagaa ttttgcggtt
    32401 cccagaagta acgccatgcc ttcgtgggca atgaaacttt catcccccgt tgcctgccag
    32461 tattgaataa ccgcccaggc gatatcagcc accaaatggt gttcagcctg cgccgaggcc
    32521 actttctggc gcagtccggt gcggatgtta atggcggcaa attccggcgt ctcttcttcg
    32581 ccgctgcgcg cgctttccca cggaaacaga gcgccctgcc agccgttgcg tcgcgctttc
    32641 tcctgcgcgc caggcaagtt gtgccagcga taacgcagta aacttcgggc aaccgtcgga
    32701 tcgctaaaca gatggaacgg tagcaaaaat acttctgtat cccagaaaac gtggcctttg
    32761 tagccttcgc cggttaaacc tttcgccgca atgctgctgc gctcatcgtg ggcaggcgtc
    32821 atgatgcgca ggtgataaag cgcataatct aacgcttgct gatcgtgcgc atcgccgccg
    32881 tttaccgtga tacgacgttt ctgccaccat tgacgccagt tttctgttga tgctgcaaga
    32941 agttggtcgt aactctgctg cgcgcacatt tcaagctgac gaagcgacgc actgccccat
    33001 tcgtctaaag cggcttgtct gtcgtcccgc cagtcgatcc acacccgttt ttgcagcgtc
    33061 agcgtctcac ctgcgtgaag ctgcgcgcag gtatgttgca gtaaacggcg ctctttggcg
    33121 gtatagcatt gctgcacatc accgctcacc ttacagcaac agctgatggc cacatcactg
    33181 cgcccatcct gggtggtgta gatcccctgc atcagatgct gaccaaatac ccgcacctgg
    33241 gtttcgtcga gatgttggcg accgtggttg gtttgcgtgg cgtcaatgcc tgttgaaatc
    33301 agcactaacg cgtcggcgtc cagtggcgta atagtgattt ccagcgcaat gagcggcagt
    33361 tggtctgccg aaacaaagcg acggctggtg atggtgtaac ctgcgccgtt gctggtacac
    33421 cagacaacgt tgcggcgtaa ttcgccactg gcaaagttaa gctcacgctg ccatgcttcg
    33481 cgggataacg agaaaatctc accatttatg gcaatctcca tccccacgac atcaggcagg
    33541 ttcaccagtt cgttgatttc acctttaccc gcccgatgat aaagccctgc cagatacatg
    33601 ccgcgcgtct ggcgcgtgta atcttcttca tggctggcgc gaagcccaag atagccgttc
    33661 ccctgcgcca tcagcgaagc atacttgttc agggtatgct ggctgaaatg gggttccgtt
    33721 aacgttactg gcctgatcat aattccacac gcgtccctgt ttctgcggat tgataaagcg
    33781 ccgccaccag ttgctggatg atgtatccct gctcggcgtc ggctatcatc acgggcttac
    33841 cttgtacgtg gttgataaag gcttccatac tgcgcagatg acggttgtcg tctgccattt
    33901 cccgttgcat cagcgtcatt aactcaccgt tgttatcggt gtagatatgt gctggaaaca
    33961 gcgtcgcccc ggctttatca ccacaaaagc tgacgttcat aatcgactgt tcgcggatgt
    34021 tgagtgcaaa tgaggtctca agccacagaa tgccgccgtt atgaaattca atggtgccaa
    34081 acagcgaatc ttcgacgctg taggttgccg ggtcccactc gccaaattga ccgcagctct
    34141 tttgcgtgcc gatcttttga aagctatgcg cagtcacgct tttcaccgct ggaaaaccca
    34201 gcacatacat cgccgcatcc agcatatgaa tgccgatgtc gatcagcggg cctccgccct
    34261 gcaattcttt attagtaaag acaccccagc cgggaacacc gcaacgacgc agggcgcggg
    34321 cggtggtaac gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg
    34381 tatcgagtgc aaaacgatgg tgaaagtcgt aggccagcac cttgccctgt ttgcgcgcgg
    34441 tatcgcacat ttcccgcgcc tgttctgccg tcatggcggg cggtttttcg cacatcacat
    34501 ggcagcccgc ttccagcgcc atcagggtat gttcgtaatg aaaacggtta ggtgagcaga
    34561 cgctaaccac atcaggtttc accgccagca gcatggcctg cggatcgtcc cacacggatg
    34621 cattcccgta tttttctgcc agcgcctgcg cctgggaaag gcggctgtca cagacagcca
    34681 ccagttccag atcgttgcgg gtgcagtagt acgaagcatg aactttatcc gccacctggc
    34741 ccgcgcctat tatcgcgacc cgcagcggag agcttgtcat tgcacttttc acaaagacct
    34801 cttagcaggt acgcaaccag gccaaagaat cacggtacgc ctgggctgga tcttccgccc
    34861 ggatgcgccc ttcgtacacc acatagccct gaaagttatc cgcgcgcagc tgttctaaca
    34921 gcgcgtggaa atccagggtg ccgctgcccg gctggtagcg atggttatcg gcaatatgca
    34981 catgaccgag caggtcgcgg ttgtcatgca gcgcctgcgc caggttatct tcttcgatat
    35041 tcatgtgata gaaatcgccg ataatctgta catgtttaag atcgttttcg acgatataac
    35101 ggcgggcatc ggcgagagtg ttgatcatgt gatcctgata acggtttaac ggttcgaggt
    35161 acaccacggt tccggtacgt gcggccacct tttccagtac gcgcagggaa tcactcacca
    35221 ttttgcggtc gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca
    35281 tgccccatgc agccggaacg acgatacctt taccgcccac ttctgccaga gcttcaagaa
    35341 tgcgttcgat ctgctttaag ccattaagtc gacgctcttc gataaagtcg ccaatccaac
    35401 cgtcatagcc accgcaggcg gtgatcaccg gtaaaccggt ttctttgatt gctgctttga
    35461 cttcttcgag attgttgacc agcagtttgc cgtcgatctc aaaaccatcg aaacccatct
    35521 ctttgatata acgaaatttc tccagaatgt tttccggaaa gaacgcctga ttctgtgtgc
    35581 cgattttcat ttttttcatt cctttgaatt aaaacgtaac gcccattttg atgctctgtt
    35641 ccggatgctg gtcgacatac tgcatgtaac tttccgggct attggcaaag gtcaccaccg
    35701 ggtcgattaa atcttcacaa ttgagataac cgttcatcag cagttcccaa caggtttctt
    35761 cgatacgttt gcggctccag cgcggataat ccgggttcgg ctcgctgcac gcacgggaga
    35821 aaacaatttt ggcgttattg aaatgggctt cgcgtccgag gttaaaacct tcggcaaacg
    35881 gtttggcaaa cgcgacatac gagatggtgc cgccatatgc cagacctcgt agcgccgatt
    35941 gcagcgcgtc ggcatagccg ctggtttcga taatcacatc ggcaccctgc ttgccggtta
    36001 gcgttttgat ctctttgcct acgtcactgc caatggggtt aaggcagaaa tccgcgccat
    36061 ggcgacgggc aatctcacag cgatgggcga taggatcgac gccaatcacc accgacgcgc
    36121 cagcacgttt agccagttga atggcaattt gaccgatcgc gccaagccct accaccacca
    36181 caaaatcgcc cacgcgcaca ttggcatcgc gcacgccgct catggcaaac tgcgccgggt
    36241 cgtagcagac agcgtttttc cacgaactgc cttgcggcat tttgcgcagc ttgtagttat
    36301 tcactgcgtt aatgatgacc gtctcggaga gcgggccgta gccgcatacg ctgtcgccca
    36361 ccgcgtagtc ggtaacgtcg ctgccgcact cgataatgtc gccaaccacc atgttgccaa
    36421 gctggaattt gccaaactca atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct
    36481 gccattcgcc gttaaagtct tcatcgataa acggactggc agcgcggaaa tcgaccactt
    36541 ccgtcccgtg cttcggtgcg ccgaagcgca cgcggatctt cacttcattg gctaaaatcg
    36601 cccgatcttc atactcaacc agcgcagcta cgcgcggtgc tgtggctact aactttttca
    36661 tgtaaaactc cttgtaaaga aactggccgc tttagccctt cacgccaccg gcggtcaaac
    36721 cactcttaat aaaacgctcc gaaagcgcat acataatcac caccggcaat gcggtcacca
    36781 gcgaggccgc catcatccgt ccccagatgt aatcaggcgt actgaacagc gcgttcaggc
    36841 cgactggtaa ggtgaaattg ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt
    36901 tccatgccac cataaagcag tagacgaata cggatatcag cccggacatc gccaggggca
    36961 cggtgatacg gaagatgatt tgcagacggt tgaggccatc catcatcgcc gcttcttcga
    37021 tctcatcagg gatggtgtcg aagtagcttt tcagcataaa cacggcggtg ggcagggttt
    37081 gcgttaccat ggtaatgatc agcgccatct cggtgtcgta tatgccaagc gcggtgatga
    37141 ttttgaacag cggcaccacc agcaaaatgc cggagaacat atacaccgtg taaaagctgg
    37201 cgttgatggt catccgccct ttaaagcgca ggcgggaaag cgcgtaagcc ccgagaatgc
    37261 cgagaaatac cgccaccacg gatgaaacca ccgacaccac catgctgtta cggaagtagt
    37321 cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacata atgctccagc gtccactgtt
    37381 gcggcagcag cgtaggatgc agcgagatcg cctcttttgc gctcttgaac gaggtcatca
    37441 gcatcacaaa aaacggaaac agcgtgatga tgagaaacag cgccagcccg cagtaaaaac
    37501 cgatgcgact gagtgtgcgt ttatttgttg ccattgaggt tcacccgttt tctggtcagc
    37561 agaatgacag cgaaaataat gatgaagagc accaccgaga tcgccgccgc tttccctaag
    37621 tcattaaagg caaaggcggt tttgtagaga tagacaccga gaatatcgac cttggtcgtc
    37681 agcagataaa catccgcgaa catgtagaac atccagatgg tgcgcagggt caccaccgtc
    37741 gccaggaccg gcataatcgc gggcagcgtg acgatacgaa accgttgcca ggcattagcg
    37801 ccatccatct ctgccgcttc gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac
    37861 gaaataaagg cataggggaa gtagcgccag atggcgaaca gcaccaccag cacaaagcta
    37921 ctgcccggat tatcgaacca cagcggtgcc tgctcataga gatgcagaag atcgacgccg
    37981 aggtagttca caatgccgta accgttgttg aacatgtatt tccaggcgaa caccagcgaa
    38041 atagacggcg ttacgtagga gaggatcacc agcgaacgcg cggttttgcg caggcggaac
    38101 tcgcggttga aaaacatcgc cacggccagc ccgagcacgg tgctgcccgc caccaccagc
    38161 gcggtatacc agaccgtcat ccacagcgag tgccagaagc cgggatcgga gagaatacgc
    38221 acgtagttgc tcaccccaac aaacgttgac tcgatgttgg gattaagcgg cagacgtaaa
    38281 aaactgattt cgatattcga caccatcggc cacgccacca gaccgcccag cagtaataag
    38341 ctgggcgcga gaagcaacag cgcaaagggc atatcggaac gacctgaaaa aagcctgttc
    38401 attgcggatt cctgtcctgg gtttagcgct gttcaaccaa ctcatccagc tttttctggc
    38461 tcgtttgcag cgtgccaggg aggtcggctt tacccacggt gacgttatgc accatggagc
    38521 tcaccacgcc agaacccgtc acatcgccca tgcgggtaaa atttttatcc cctactgcgc
    38581 caaagacctg aatatttggc agttcgctga ttaactgatt tggcagctca cccagcgcct
    38641 taataacgtc gttgtctttc caggtggcgg tagtgacaac cgctttgttc accggcagcg
    38701 ccgcgcctgg cgacatcatc acccagtcgg caatgttgtc tgcctgctcc ataaaggtga
    38761 caaatttttc agccgcttcc gtctcttcgg ccttttgccc gacggtaatg gtcagcgagg
    38821 tcaacatgcc gtagaccgca gagtttttct cggtgggtac aacgaaacca acgtttttcg
    38881 gatcgccttc tttaatcaca gccggaagga tataggtgga gtaaatcgcc atcggcgcgg
    38941 tgccgttcat aaaagcgtct ttcacttcca tgatgtcgtt agaacctggc atggtgttgg
    39001 cagcaaggtt gcggtaatag gtcagcgcct gcatcatctc tggtgtatca agggtgattt
    39061 tgccttcggc gttaaagacg ttagcctgat tggataacgc aaactgggag aaagactgtt
    39121 ccgtcaacac gctttctgct gtaggcagcg caataccgta ttttttattc gccgggtcat
    39181 tcagtttctg tgcaacgtcc agcaattgct gccagttttt tggctcctcc agcccagctt
    39241 ttgccagcac atctttgcga taccagatcc cgccaatcca tgcgctgaca ggaacaccgg
    39301 tccaggcgct gccatcttcg gtgcgcacaa tccgcagtac gccatcgtag aacgcgcctt
    39361 caccgacgtt gctgatgacc gtggcaaccg ctttacgatc gataagctgc tctttgtcca
    39421 tcactttggc gtagtcatgg ctggtttcga tcacttccgg cagcgaaccg ctacgtgaaa
    39481 gggtaatgac tttggtgtta taggcgtctt cttccacggg cacttgtttt acggtgatac
    39541 ctgggttttc tttttcaaaa cgggcaatca atttgctgat gacggcctgg cgctcctgct
    39601 ccaccgaaga atgcataaat tcgatggata cattattttt attttcttct ttacagcctg
    39661 aaatcagggc gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgaaat
    39721 gttccttttt atttaatcca cataacctgc cagggatgca gtgttatttc attacccaat
    39781 atatcaactt cactaatcag atcccgaccg tgcaggtcag taatatttat atgctgaata
    39841 ttttcactga cattaaacaa tcccgtcagg caatcgccat ccgcattgct tctttgaatt
    39901 cgcattacaa atgaattaat cgcatcaatg gtgaaattat tatccggatg aaactcttta
    39961 tgactgcgac gaagtgtaat taaatgcgac aacttataat atatcaaata ccttaatgtc
    40021 gctttattgt tcagttctgt cgttatctct ttactagagt attttttacg gttaatcgca
    40081 cgattatatc caagtttttc gacacctgcg taatcattac gcgaccccag aatgctttga
    40141 atatatatcg ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg
    40201 gcacaccgtt cttcgtcgct acactcacgg cggctcaacg catccatata ggtgacattc
    40261 atttcatagg ggctacgcgt accgtcggga ttatttttcc agtttactaa tgccccttcc
    40321 tgctgtaaca cctcgaccag cgctaatatt tcgttttcgg gcaacaaccc ccgcagtggg
    40381 ttaagcccga tgccatcgtg agaggcaagg aagttaaacc aggtggtgtt gctggaaggt
    40441 agtgtcaggt tttgcgccca cgcacaaagt gcctcaacgt tctgtttttg caccgcatgc
    40501 agcaccagcg gcggcagcga gaactggtac accatatgcg cttcgtcatc accgttgcca
    40561 aagtaagcaa tgttgtctat atgcggaaca ttggtctcgg taatgatcac tgtgcctggc
    40621 gcaacgttat caataatcga ccgtaacagt ttgataatca gatgtgtttt ttccagatgg
    40681 atgcagcttg ttcccggctc tttccacata aagccaacgg catccagacg gatatactca
    40741 gcacctttct caaggtaaca cagtaaaaca tccaccatcg ccagtaacac ttcagggcta
    40801 cggtaattga ggtcaatttg atcgtcgcta aaggtggtcc ataaatgacg cgttgaatta
    40861 tcgcgcatct ggaatggcgt taataacggt aacgcacgcg gacgggtgac ggcgctgaga
    40921 tcggtttgcg gatcaaccgc aataaaaaaa tcttcaaaac ctggctgctg ttgtaaatag
    40981 tttttaaacc attcactttt tgccgacata tggttgcaga caaaatcaaa cattaaatga
    41041 ctgcattcac cgagttgctg aatatcctgc cactcccccg cttcactggc gacctgatga
    41101 taatcaatta ccgaaaagcc atcatcagat gaccatggat aaaacggcaa caaatgaaca
    41161 tgtgaaaaag tgctttgcag ccattgatgg taaaactgat taaatgtggg tagtggtttt
    41221 aaatcattac tgtgaaattg atcggcatag gtaattaaca cgacatcact ttcatcccag
    41281 tgttttttac gtcgctgtgt aattaatcgt ttcgcactct caagacgcgt tacaagtttc
    41341 tgtaaatgag tcgcggtaaa tgttccaccg tagatttcgt ccaggtaatc cgtaattttc
    41401 tgttccatgt tgccttttcg tggtagcggt cccatgaaac gagagactac tccgcagaaa
    41461 ataaactgtc aattgagcac aagggtttta gtcacgcagg aaatcatttt ttaccaggga
    41521 aaaagcgtat ttgtgagctg acgcaagtaa tctatgggga tgtaaataag gtgtgacaga
    41581 aaaagtaaac ggcgcataag cgccgcttag ggtggattct tttaaccttt gaccttcggc
    41641 attgcgatgt ctttcaggcc gccggcgttc tcaacgtggg tatatcccat ctcgctaagg
    41701 atctcttttg cctgccctga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt
    41761 ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggct
    41821 ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca gtgttcagcg
    41881 gcgaaaacgg gcagtgaaaa caccagtgtc agagcaagta agcctttttt aaacatgtaa
    41941 gcgtccttct atggatgaaa attgccatcc ataatacgcc ttagctttaa caatttttgc
    42001 gaaactcccc tcacctttta taacgctgcg ccagtttatt ggcagcccga cttaacagcg
    42061 gttccagcgc cacagccagc aacattttca gtggtcggcg agcaactgat tttatcgccc
    42121 agccagctac acccgccggg ccatagcgca gtgcggtaag aagtaccagc ttgcctgcta
    42181 atttgaaacc aggcttcacc ttttgcccgg cctgttgcca gcgagtattc ataactttcc
    42241 tcacagttga cggaaacggc tacgtaacgt gaaagtatcg gaagtgacat aacgttccat
    42301 ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg acttcatcca gcaattcact
    42361 gctggaaggt agctgctcac caaaggccat gttgtccggc attggatcaa gcgcaaatga
    42421 caaaatgatg taagcaacca gggtaaacag cgccagaccg aagaaaatcg acagcaccac
    42481 caggatacgc accagtttta ctggtacatc aaaatagttg gcaatcccgg cgcagacgcc
    42541 acggaccatg ccctgctgtg gaatacgcca taatttttta ttgagattaa tgcccgccat
    42601 tagcgatccc tccagttcgg atgttctgca tcaagaatgg attccagcgc ctgaatacgt
    42661 tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc gctgctgctc actttgcgac
    42721 aattcactgc gaccagaacg attgctgtaa tgcagccata accagatcgg taaaacaaac
    42781 agcacaaaaa tggttaacgg aatagccaga aatagcgcgc tcataaggac tccttagacg
    42841 atgagcggcg gcgcgttcgg acgccgcctg gatgttatta ttgattgtct tgcttcattt
    42901 tggcttttaa ttgtgccagt tgctcgctga ttgcatcatc ggctttcagt tcggcaaact
    42961 gatcgtccag cgatttttgt ttaccgaagc tgtggctttc tgcttccgct tccatctggt
    43021 caattcgacg ttcgaaagat tcgaaacgag ccattgcttc atccagtttg ccactgtcca
    43081 gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg gcgtaacatc aatgcctgct
    43141 ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc gatctctttc ttcatgcgtg
    43201 ccagcgtatc gtccaccagc gtcacttcat gttccaggct cttaatcaga tcggtcagtt
    43261 tctgtttttc aattaacgcc gcacgcgcca ggtcttctct ctctttcagc agcgccagtt
    43321 cggctttttc ctgccattca acttcacgcg ccgacgcttg ttcaatacgg cgagtcagct
    43381 gtttcttttc tgccagcgca cgcgccgaag tagaacgtac ttcaaccagt gtatcttcca
    43441 tctcctggat catcagacgc accagtttct gtggatcttc cgctttctct aacagagcgt
    43501 tgatgttggc attcacgatg tcggcaaagc gagaaaaaat acccataatg ttgtcctctt
    43561 tatttctgcg ttcaggattg tcctgctgaa ctattaatac aatttgcgtg ccaatttttt
    43621 atctttttga tttataaaga tctgattgaa gaatcaacag caacatgcca ggatgagtta
    43681 gcgaattaca ctaacaagtg gcgaatttca tcatggcaga atacaaagat aatttactcg
    43741 gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc gcatctcgca ccgctggata
    43801 aaccggtgct catcatcggc gagcgcggca caggtaaaga gctgattgcc agccgtctgc
    43861 attatctctc ctcccgttgg caggggccgt ttatttccct taactgcgcg gcgttaaatg
    43921 aaaatctgct ggattccgaa ctgtttggtc acgaagcggg ggcgtttacc ggtgcgcaaa
    43981 aacgtcatcc agggagattt gaacgtgccg acggcggtac gctatttctt gatgaactcg
    44041 ctacggcacc gatgatggtg caggagaaat tattgcgcgt gattgagtac ggtgaactgg
    44101 agcgcgttgg cggcagccag ccattgcagg tgaatgtacg gttggtatgc gcgacgaatg
    44161 ccgatctccc ggcgatggtc aatgaaggta cttttcgcgc tgacctgctc gaccggctgg
    44221 ctttcgatgt agtgcaactg ccgcccctgc gcgagcgcga aagcgacatt atgctgatgg
    44281 cagaacactt tgccattcag atgtgtcggg aaatcaagct gcctctgttc ccgggtttta
    44341 cggagcgcgc cagagaaaca ttgctgaatt atcgctggcc gggaaatatt cgtgaattga
    44401 aaaacgtggt ggaacgttcg gtgtatcgcc acggcaccag cgattatccg cttgatgaca
    44461 tcattattga tccctttaaa cggcgtccgc ctgaagacgc tatcgccgtt tcagaaacca
    44521 cctcgcttcc aacgctgccg ctggatttac gtgagtttca gatgcagcag gaaaaagagt
    44581 tgctgcaact cagtttgcaa caagggaaat ataaccagaa gcgcgcggct gaattactgg
    44641 ggttaaccta tcatcagttc cgcgcgttgt tgaaaaagca ccagatttag cgaagttggg
    44701 caatacaact tgccggatgc ggcgtgaacg ccttatccgg catacataat caatacgttg
    44761 tttgcaggcc tgataagcgc agcgcatcaa acattattgc gttgggctat taatcgctca
    44821 gcgcatcctg caaaattttc attgcctcat cgaattgcgc atccgggatg gtcagcggat
    44881 acaggaagcg aatcacgttg ccgtatgcgc cacaggtcag caagagcagc ccctgcgcca
    44941 gcgcgcgttg ctggattttc tgtgcaatcg ccgctgacgg ctcgcccgtt tgcggatcgt
    45001 taaactctgc cgcaatcata gaccccagac cgcgtaccgc cgcgatggcc ggaacgcttt
    45061 ctttggcatc aatcaacgtg tttgtcaggc gctggcccag ttgattcgcg cgttcacaga
    45121 gtgattcttt gtcgataatg ttgagcacag cgtgcgcggc agccaccgcc agcgggttcc
    45181 cggcgtaggt accacccaac ccgcccggcg cgggcgcgtc cataatattc gcgttaccga
    45241 ccacgcccga aagcggcatc ccgcctgcga ggcttttcgc catcgtcatt aagtccggct
    45301 tatcggcgta gtgatccatg gcaaacattt taccggtacg cgcaaagccg ctttgcactt
    45361 catcggcaat catcacaatg ccgtgctcgt cgcacaggcg gcgaatggcg gcaaccagct
    45421 cttttggcgc aacgttgaaa ccgccttcgc cctgaaccgg ttcgaaaata atcgccgcca
    45481 cctgcttcgc ttcaatgtcc gatttaaaca agcgttcgat ggcgtcgagg gagtcctgcg
    45541 ttgaaatgcc gtgtaaatct gacggataag gtacgtgata taccgaaccg gggaacgggc
    45601 cgaagccgat tttgtacggc gcgacctttc cggtcagcgc catggtcata tatgtacgac
    45661 cgtggaagcc gccgctaaac gcaatcacgc cagggcgtcc ggtatgggcg cgggcgattt
    45721 tcaccgcgtt ctccaccgct tccgcaccgg tggtgaagaa cgcagtttta gcctgcccgc
    45781 tcaccggggc aagggcgtta attttctccg ccagcgtgac gtagctttcg tacggcacaa
    45841 tctgatacgc cgtgtgggta aactgttgca attgctgttc caccgccgcg accagatcag
    45901 ggtggcgatg cccggtattc agtaccgcaa tgcctgcggc gaaatcgata tattcgttgc
    45961 cctctacatc cttcagcgtg gcgttttctg ccgactgggc gaagaagtta cacatcacgc
    46021 caaccccgcg tggggtagca gaaagacgac gctgatggaa ttcattgttg ctcatgtttc
    46081 acaccttttg actcttcact gttaatcgga aattaaacgc ccagacgatc gcgcaggctg
    46141 taataagccg cgcccatcgc ggtaaacgga acgcgcagcg tgcgcccgcc ggggaacggg
    46201 taatgcggca gattggcgaa ggcgtcgaaa cgctcggcgt caccgcgcag cagttcagca
    46261 atcaaacgcc cggcgaggtg ggtacaggtg acgccgtggc cgctgtagcc ctgcatgtag
    46321 tagatgttgg tatcgaggcg accaaactgc ggcatgcgtg acagggtcag caggaagttg
    46381 cccgtccagc ggtaatcaat tttcacgccc ttcagttgcg ggaaggtttt cagcagtttc
    46441 ggcaccacaa ggcgctcaac atcgtccggg tcgcgtgcgc cgtagaccac gccgccgccg
    46501 tacagcaggc ggttatcggc ggtaagacgg tagtaatcaa gcagatagtt acagtcttcc
    46561 acacagtagt ttttcgggat cagcgaacgg gctaaatctt ccgacagccg ttcggtggtg
    46621 atcacctgcg tgccgcacgg catgctgcgt ttcgctagtt ccggctctac tttatcgccc
    46681 aggtaggcat tcccggcgac aatcacatac ttcgccgtca cctgaccatt ggcagtccgc
    46741 accacggctg gcgtggtgtg ctggatctgc gtcactgcgg aaagctcatg cacgcgcccg
    46801 ccgttcaggc ggatggcatc ggcttcacca atggcgaggt ttagcgggtg aatatgccca
    46861 ccgctgtgat ccagcagcgc accagtgtag cgatcgctgg cgacctcgcg gcgaatagcg
    46921 ttggcgtcca gcagctccag ctgtttattg ccgtagcgtt cccagttctc tttctgctct
    46981 tcgagtgtgg cgagctgttt atcattcatc gccacaaaca gaccgccggg gcgatagtcg
    47041 cagtcaatct gataacgttt gatacgttcg cggatgatct cgccaccctc gaacatcatg
    47101 ctgccgagca tacgggcggt atccatgccg tagctttttt cgatcacgtc gatgtcgcgg
    47161 ctgtaggagt tcacaagctg cccgccgttg cgtccgctcg cgccaaagcc gatgcgtgag
    47221 gcttcgagaa ccaccacgtc aaagcccgct tccgccagat gcagcgcgga ggaaagcccg
    47281 gtatagccgc cgccgaccac gcaaacgtcg caggaaatcg actcattcag cgtgtcgaat
    47341 ggcgcgtatt tattcgcact ggcggcgtag taactgctgg tgtgttcggt catttcaggc
    47401 ctccaggctt atccagatgg ttttcagttc ggtgaatttt tcaagggcat gcagggattt
    47461 gtcgcgcccg ttgccgctct gcttatagcc gccaaacggc acggtcatat cgccgtcgtt
    47521 gtagttattg acgaagacgg agccggcttt caggcggcga ctcatgcggt gcgcgcggga
    47581 gaggtcgcgc gtccataccg ccgcgccaag tccgtactgg ctgtcgttgg caagctgtag
    47641 cgcctgttct tctgatgtga aacgcgtgac caccagcacc ggaccgaaaa tctcttcgcg
    47701 gcttaaggac gcattcgggt ctacctccac aaagatggtc gggccgatgg cggcagccag
    47761 ctcggcgtta cggccatcca gcagcagttg ccctttgctt tcgccttccc gaataaagct
    47821 atggaccgag tcggcgtggg cgcagtcgat taaggtgccc atggtggttg cgggatcaag
    47881 cggatggccc ggctgccagt tttgcgcttg ctgttttaac agggctaaga attcatcggc
    47941 gatgctctct tccagcaaca ggcgcgttcc ggcgatacac acctgcccct ggttgtagaa
    48001 aatgcccgca gcggtcgcgc tcgccgcttt ttgcaaatcc ggacagtcgg cgaaaacgat
    48061 gttggcgctt ttgccgcccg cttccagcca gacgcgtttc atgttgctgt cgcccgcgtc
    48121 cttcagcagc tgtttcccgg tgcgggttga gccagtaaag gcaatggcat cgatatcgtt
    48181 atggcgcgaa agcgcctgcc cggcttcatg accaaaaccc gtcaccacgt tcaacacacc
    48241 atccggcagc cccgcttctt tcgccagccc cgcgagacga atcgcactga gcggtgattt
    48301 ttcagacggt tttagaatca cgctgtttcc cgccgccagc gccgggccga gtttccagca
    48361 agtcagcagc agcgggaagt tccacggcac gatggcggca atcacgccga ccggttcacg
    48421 cacgatcatc gccagctcat ggctactggt ggtcgccact tcgccataca ctttgtcgat
    48481 cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga atatcatcac gcaggctgtg
    48541 acgaatcggt ttaccggtgt cgagcgtttc cagcagcgcc agttcttcag cgttggcttc
    48601 cattaaatcg gcgagtttat tcagcaccgc tttacgctta gccggagaag agagtgacca
    48661 gtcgccacgt tcaaacacgc cgcgtgctgc gctcaccgca cggtcaatat cgacgctctt
    48721 gccgcgggca atattcgcca gcggtgcctg ggtgaccgga tcaacggttt caaaagtttc
    48781 attttccgcc gcagcagtat attcaccgtt aataaataag cgggtttcaa tggcgagact
    48841 taacgcttta tcctgccagt aagccagatg atgaaaattc attatgactc ctgtttcacg
    48901 tctatcagat atatgcagtt tgtgaacgtc agggcgcaga gatccccggg ccgtatgccc
    48961 gtttatttct ggcgacattc tttatagatt gccgggcgca ttgcgcttac ccggcttaca
    49021 gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga taattcggca aatacctgcc
    49081 gacgtattac tgaaactgtg cgggatgccg gtattaatgg cataactttg ccccgcgacg
    49141 agatgataat cctgaccatt aatcgtcaga acaatttcac cttccagtac agtgcctatt
    49201 tcctcaccct gatgcttaat tctttcccca gtggttgtgc ccggctggta agtttcaaag
    49261 atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca gcttcattga cacaccctga
    49321 ctgcccatct caattaagtc gtcctgatta atgacgacct gcggctcatc aggtttttcc
    49381 ggctcggaaa agaattccga gagtgacaga ccatacacct tcagcagctt ttgcagcgta
    49441 ctgatggcag ggctgacttt atcttgttct atcgtactga tagcactgtg agtcagcccg
    49501 gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct ggcggatttc cgacaagcgt
    49561 tttcctggcg ccagtccctc atcactcata tttgcatttc cttaaactgt agtggtcaga
    49621 gtcgctgttt ttcagcgata tggtgctgac aagcggtgat aaagccctcg aacaatatac
    49681 gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca ctgtacgccc agcgcaaaag
    49741 gatgattgat gacgctaacc gcctcaacta agccatccgg cgagcgagct tcaacacgca
    49801 accgtgggct aacgaccttc gccccttgtc catgcagaga gtttacccaa aagttgctac
    49861 attcaggtaa caacgcagac agtaatcccc cctcttcaac ctgaacttca tgcgacggcg
    49921 catattgctg ctccaccggc agttcgggat cttcccgatg ttccagcaat tcaggctgtt
    49981 cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcctgt aaaccccggc
    50041 agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattaacgcc atgctcagaa
    50101 gatcacgccc aggatcggcg tcaggctcat cgccgttttc accatatagg tgcggctgca
    50161 cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt tgttcaagta
    50221 atgacggttc cgccagcgca tgtggcagcg caattggcaa gccgcctgcg tggatgatgg
    50281 cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg ttcctgcaca
    50341 ttacgacacc gataaccgga ttgttcatta tattttccat gctcaatctc acaaagtgga
    50401 ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt tttcaatcta
    50461 gcagtgggtt ttcatttttg caaactcaat ttaacatttg acaaacattt agtttgcata
    50521 cagattcgaa tagtggtcat tatattttac gctttgataa cgagcggaaa acaaaccaaa
    50581 ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag cagtcagaag
    50641 agaggcgggg tagctccttt acgcaggaag tgaaacgcta cctggagcgc tacccgaata
    50701 cgcaatatgt tgatgtttta cttaccgatt tgaatggttg cttccgtgga aaacgtattc
    50761 ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt cccggcctcg gtgtttgcaa
    50821 tggatattct gggcaacgta gtagaagaag cgggcctggg tcaggaaatg ggcgagccgg
    50881 atcgtacctg tgttcctgtt ctcggttcct taaccccttc tgccgccgat ccagagttta
    50941 tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac gttgagccgc
    51001 ggaacgttct taaccgcctg tggcagcagc tgcgccagcg cggattgttc ccggtcgtag
    51061 cggtagagct ggagttctat ttactggatc gccagcgcga cgctgaagga tatctgcaac
    51121 cgccctgcgc gcccggcacc gatgaccgca atacacaaag ccaggtttac tccgttgata
    51181 acctcaacca cttcgccgac gtgctcaatg atattgatga gctggcgcag ttacagctga
    51241 ttccggcaga tggcgcggtc gccgaggcct cgccaggtca gtttgaaatc aatctttacc
    51301 ataccgataa cgtgctggaa gcttgcgatg atgcgctggc actaaaacgc ctggtgcgtc
    51361 tgatggcaga aaagcataag atgcacgcca cttttatggc gaagccgtat gaagagcacg
    51421 cgggcagcgg gatgcatatc catatcagta tgcaaaataa tcgtggcgag aacgtgcttt
    51481 ctgacgcgga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg atgattgacc
    51541 tgatgccgtc atcaatggcg ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc
    51601 cggggatgta tgtaccgacg caggcgtcgt ggggccataa caaccgcacc gtcgccctgc
    51661 gtattccctg cggcgatcgc cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg
    51721 ccaacccgta tctggtgatg gcggcgattt ttgccggtat tttgcatggc cttgataacg
    51781 aactgccatt acaggaagaa gtcgaaggta acgggctgga acaggaaggc ttacccttcc
    51841 cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt
    51901 taggcgaacg cttctgccat gtctatcacg cctgtaaaaa tgatgagctg ttgcagtttg
    51961 agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcgtaa taccccttgc
    52021 ggggttgttt catcggcctg agcggagcca ggcctgacat ttgctggtaa tccagacatt
    52081 tcccggatgt cgcgttatgg agcgcaggcg gcaacgggct tccgttaacg attaagactg
    52141 tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg aggctgcgta
    52201 tgcgcccctc ctgcgactga ctggactccg ccccctctgc ttcccttgct aaacaggttt
    52261 gatttcaggt cgacacgacc gcaaacctta ttacgcaggg gaggcagcaa ttatggctat
    52321 taattcacca ctgaatattg ctgcgcaacc tggcaaaacc cgtctgcgaa aatcactgaa
    52381 attgtggcag gtggtgatga tgggtctggc ctatctcacg ccgatgaccg tatttgatac
    52441 ttttggtatt gtctccggca ttagcgacgg tcacgttccg gcgtcctatt tgctggcgct
    52501 ggcgggcgtg ctgtttaccg ctatcagcta cggcaaactg gttcgccagt ttccggaggc
    52561 cggttcggcc tatacctacg cgcaaaagtc gattaacccg cacgtcggat ttatggtcgg
    52621 ctggtcatcg ctgctggatt atctcttttt gccgatgatc aacgttttgt tggcgaaaat
    52681 ctatctctcc gccctcttcc cggaagtgcc gccgtgggtg tgggtggtaa ccttcgttgc
    52741 cattttaacc gccgcgaatc tgaagagcgt caacctggtc gctaacttca ataccttgtt
    52801 tgtactggtg caaatctcca tcatggtggt gtttatcttc ctggttgttc agggactgca
    52861 taaaggagaa ggcgttggca ccgtctggtc acttcagccg tttatcagcg agaacgcgca
    52921 cctgatccca attattaccg gggcgacgat tgtctgtttc tccttcctcg gtttcgatgc
    52981 ggtgaccacg ctttcggaag agacgccgga cgccgcacgc gtgatcccga aagccatctt
    53041 cctgacggcg gtatacggcg gcgttatctt tatcgctgcg tcgttcttta tgcagctgtt
    53101 ctttcccgat atcagccgct ttaaagaccc ggacgccgca ctgcctgaaa ttgcgctata
    53161 cgtcggcggc aagctcttcc agtcgatttt cctctgtacc acgtttgtga acacgttagc
    53221 gtctggcctg gcgtcgcacg ccagcgtgtc gcgtctgctg tatgtgatgg ggcgtgacaa
    53281 tgtgtttccg gagcgcgtgt ttggctatgt gcacccgaaa tggcggactc cggcactgaa
    53341 cgtcattatg gtcgggattg tcgcgttgtc agcgctgttc tttgatttag tcaccgcgac
    53401 agcattgatt aacttcggtg ctctggtggc atttaccttc gtaaatctgt cggtgtttaa
    53461 tcatttctgg cggcgtaaag gaatgaataa aagctggaag gatcacttcc actatttgct
    53521 gatgccgctg gttggcgcgc tgacggtggg tgtgttgtgg gttaacctcg agtcaacgtc
    53581 actgacactc ggtctggtat gggcttcgtt aggcggcgca tatttgtggt atctgatccg
    53641 ccgctatcgc aaagtgccgt tgtatgatgg tgacagaacg ccggtgagcg aaacgtaata
    53701 gtcggatgcg cggcgcatta tcctgcctga gggtaatgca ccgcgccatt aactataaaa
    53761 agatgtatga cccggatcac gagtgctatc atccgccctt atgcttagcg cattaacccc
    53821 aggggcaaat aatgaaccac gatatcccgc taaaatattt tgatattgcc gatgagtacg
    53881 cgaccgaatg cgcagagcct gtcgcagacg ctgaacgcac accgctggcc ctctacttcc
    53941 agttactgct cacccgtttg atgaacaatg aagagatcag cgaagaagcc caacatgaaa
    54001 tggctgctga agcggggatt aatcccgtac gcattgatga gatcgcagag tttttgaatc
    54061 aatggggtaa tgagtaatta tttcattgca gactctcggt cagtatcgtt gtccggataa
    54121 agcgttcacg ccgcatccgg ctaacaatga cacgcactgc agcgtgcctc aatgggtttc
    54181 tcacgatcgt accgctctgc tcctcgctaa gttgttctgt gagttatagt tatagagaaa
    54241 tcacgggtac gaatgttgaa cagatggcga cgtcatctgt tcaacgacca accacacggc
    54301 cctcattacg acccctgaac acaaacggta atggcaaatg tcattaaata ttagtaagat
    54361 agcctcttta cataaaatcc attaatatta tgcgccaggt attatcgtct cttttggtga
    54421 ttgctggact tgtgagtggt caggcaatcg ccgcgcctga atcccccccg catgctgata
    54481 tccgcgacag cggttttgtc tattgcgtca gcgggcaagt caacaccttt aacccatcca
    54541 aagcgagcag tgggttaatt gtcgataccc ttgccgccca gttttatgat cgactgctgg
    54601 atgtcgatcc ctatacctat cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg
    54661 acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact
    54721 ggtttactcc cactcgtaaa atgaatgccg acgatgtggt gtttaccttc cagcgaattt
    54781 ttgaccgcaa caacccgtgg cataacgtca acggcagcaa cttcccctac ttcgacagcc
    54841 tgcaatttgc cgataacgtg aaaagcgtcc gtaaactgga taatcatacc gttgagttcc
    54901 gtctggctca gccggatgct tcttttttgt ggcacctcgc aacccattat gcttcggtca
    54961 tgtcggcaga atatgcccgg aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc
    55021 agccggtcgg cactggaccg tatcagttgt cggaataccg cgccgggcaa tatattcgct
    55081 tacaacgtca tgatgatttc tggcgtggta agccgttgat gccacaagtg gtggtggatt
    55141 taggctctgg cggtacagga cgattgtcga aactcctgac cggggagtgc gacgttctgg
    55201 cctggcctgc tgccagtcag ctatccattt tgcgtgacga cccacgcttg cgtttaacgc
    55261 tgcgcccggg gatgaacgtc gcctatctgg cgtttaacac cgctaaaccg ccgctaaata
    55321 accccgctgt ccgccatgcg ctggcactgg cgattaataa ccagcgcctg atgcaatcca
    55381 tctattatgg tacggctgaa acggcggcct ctattttacc gcgcgcctcg tgggcctatg
    55441 acaacgaggc taaaattact gaatacaatc cggcaaaatc gcgcgaacag ttgaaggcgt
    55501 tggggctgga aaacttaacg ctgaaactgt gggtgccaac acgttcgcag gcgtggaacc
    55561 ccagtccgct gaaaaccgcc gaactgattc aggcggatat ggcacaggtt ggcgtaaaag
    55621 tggtgattgt gccggtagaa ggtcgttttc aggaggcgcg gttgatggat atgagccatg
    55681 atctgacgtt atccggttgg gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt
    55741 tactgagctg cgcggcaatt cattcacaga ccaacctcgc ccactggtgc gatccgaaat
    55801 tcgacagcgt gttgcgtaag gcgctctcct cgcagcagct ggcggcgcgt attgaagcct
    55861 atgacgaagc gcagagtatt ctggcgcagg aattacccat tctgccgctg gcgtcgtcat
    55921 tgcgcttgca ggcctatcgg tacgatatca aaggtctggt acttagcccg tttggtaacg
    55981 cctcctttgc tggggtgtat cgcgagaaac aggatgaggt gaaaaagcca tgattatctt
    56041 caccttgcgc cgtattttgt tattgattgt caccttgttc ctgctgacct tcgttggctt
    56101 cagcttgagc tatttcaccc cacatgcgcc gttacaaggc gcgtcactgt ggaatgcctg
    56161 ggtgttctgg tttaacggcc tgatccactg ggattttggt gtttccagca ttaacggtca
    56221 accgattgcc gaacagttaa aagaggtctt cccggcgacg atggaactgt gcatcctcgc
    56281 cttcggtttt gcgctgattg ttggcatccc cgtaggaatg attgccggga ttacgcgcca
    56341 taagtggcag gataacttga tcaacgccat cgccttactg ggcttttcaa tcccggtgtt
    56401 ctggctggcg cttctgctga cgctgttttg ttcactcaca ctaggttggt tgcccgtttc
    56461 agggcgtttc gatctgctct acgaagtgaa accgattacc ggttttgcgt tgattgatgc
    56521 ctggctttcg gactcaccat ggcgggatga aatggtcatg agcgcaatcc gccatatgat
    56581 attgcccgtg atcaccctgt cggttgcgcc aacaactgaa gtgatccgac tgatgcgtat
    56641 tagcactatc gaagtgtatg accagaacta tgttaaagca gcggcgaccc gcggtttgtc
    56701 gcgctttact attttgcgtc gccacgttct gcataacgcg ttacctccgg ttattcctcg
    56761 cctgggctta cagttttcta ccatgctgac gctagcgatg atcaccgaaa tggtttttag
    56821 ctggccgggt ttagggcgct ggttaattaa cgccatccgc cagcaggatt acgcagccat
    56881 ttccgccgga gtgatggtgt gtggctcact ggtcattatt gttaacgtga tttctgatat
    56941 tttgggtgcc atggctaacc ctctgaaaca taaggaatgg tatgccttac gatagcgtat
    57001 acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg
    57061 cctctgcgat ggtcggcctg tacggctgcg cggggctggc tgtactgtgt atttttggcg
    57121 gctggtttgc gccttacggc atcgaccagc aatttctcgg ttaccaatta ttgccgccgt
    57181 catggtcgcg ctatggcgaa gtttctttct tcctggggac tgacgacctc ggacgcgatg
    57241 ttttaagccg tttactgagc ggagctgcgc caaccgtagg tggcgcattt gtggtgacgc
    57301 ttgccgcgac gatctgtggc ctggttctgg gcaccttcgc cggggcgacc cacggcctac
    57361 gctcagcggt gcttaaccat attctggata ccttactggc gatcccttcg ctgctgctgg
    57421 caattatcgt tgtagcgttt gccggaccga gtttgtctca cgccatgttt gctgtctggc
    57481 tggcattgct gccgcgtatg gtacgttcga tttacagcat ggtgcatgac gaactggaaa
    57541 aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac gctgaatatt ctctggtttg
    57601 ctgtgatgcc caacatcacc gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg
    57661 caattctcga tatcgccgcg ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg
    57721 aatggggagc gatgctcggt gatgcgctgg aactgattta tgtcgccccg tggactgtca
    57781 tgctgccagg tgcggcaatt atgattagcg tgctgttggt taacctcctt ggcgacggtg
    57841 ttcgtcgtgc aattattgcg ggggtggaat aatgccatta ctcgatattc gtaacctgac
    57901 cattgaattt aaaaccggtg atgagtgggt taaagccgtc gaccgcgtaa gtatgacctt
    57961 aaccgaaggt gaaatccgcg gtcttgttgg cgaatccggt tccggcaaaa gtttgattgc
    58021 gaaagcgatt tgtggggtga ataaagataa ctggcgtgtt actgctgacc gtatgcgttt
    58081 tgatgatatc gatttgctgc gtctctccgc acgcgaacgg cgcaagctgg ttggtcacaa
    58141 cgtgtcgatg attttccagg aaccgcagtc ttgtcttgac ccttcagaac gtgtgggtcg
    58201 ccagttgatg caaaacatcc cagcctggac gtataaaggc cgttggtggc aacgttttgg
    58261 ctggcgcaaa cgccgtgcca ttgaactact gcaccgtgtg gggattaaag atcacaaaga
    58321 tgcgatgcgc agttttccct atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc
    58381 cattgcactg gcgaatcaac cgcgtctgct gattgctgac gaaccgacaa actcaatgga
    58441 gccaacaacc caggcgcaaa tctttcgcct gctgacgcgt ctcaaccaga acagcaatac
    58501 cactattttg cttatcagcc atgacttaca aatgcttagc caatgggcgg ataaaattaa
    58561 cgtgctttac tgcggtcaaa cggtggaaac cgcgccaagt aaggagttgg tgacaatgcc
    58621 acatcatcct tatacccagg cgctgatccg cgcgatacca gacttcggca gcgcgatgcc
    58681 gcataaaagt cgcctcaata cgctgcccgg cgctatccca ctgctggaac agttaccgat
    58741 tgggtgtcgt ctggggccgc gttgcccgta tgcacaacga gaatgcattg tgacgccacg
    58801 tttgacgggg gcgaaaaatc atctctatgc ctgtcacttc ccgctgaaca tggagaaaga
    58861 gtgagatgat cgaaacgctg cttgaagtgc gtaatttaag taaaacgttc cgttaccgga
    58921 ccggctggtt tcgtcgtcag accgtagaag cggtaaaacc tttgagcttt acgctacgtg
    58981 aaggccagac actggcgatt attggtgaga atgggtcggg taaatccaca ctggcaaaaa
    59041 tgctggcggg aatgatagaa ccgaccagcg gcgagttatt gattgacgat catccgctgc
    59101 attttggcga ttattccttc cgtagtcagc gtattcgcat gatttttcag gatccttcga
    59161 cctcgttgaa tccccgtcaa cgtatctcgc agattcttga ttttccactg cgcttgaaca
    59221 ccgatctgga acctgaacag cgtcgcaaac agattattga aacgatgcgg atggttgggc
    59281 tgctaccgga tcacgtcagt tactatccgc atatgttggc gcccggacaa aaacaacgtc
    59341 tgggtctggc acgcgcgctg atattgcgtc caaaagtgat tattgccgat gaagccctcg
    59401 cctcactgga tatgtcgatg cgttcgcagt tgattaatct gatgctggaa ttacaggaaa
    59461 aacagggtat ttcgtatatt tatgttaccc agcatatcgg aatgatgaag cacattagcg
    59521 accaggtact ggtgatgcat cagggcgagg ttgtcgagcg aggcagcacc gccgatgtgc
    59581 tggcatcgcc gctgcatgaa ctcaccaaac ggcttattgc cggtcatttt ggcgaggcat
    59641 tgacggcgga tgcgtggcgt aaggaccgtt agtggtttgc acctcatcct gacagggccc
    59701 ccctcaggat gaggtgttca ttacttttga ttgtcgattc tggtgagagc aaccagcaat
    59761 gccagcgtat aacacaccgt aattgccaga ctcattttta acagtgtttc tccgccaatt
    59821 cccgacaatg gcgcggctat accgccgaag acaaacatgc tcatccccat caacgcagac
    59881 gccgttccag attcctgcgt ccctactgca ctcatagcct ctgccccgcc aacagtgccg
    59941 atgccgctac aaaatgcgat ggtgaaaaat aatgccacca gtgccggaat gggcaattgt
    60001 atccatgcgc ataagaccgt gagcaatgca cacaaaattg ccgctatcag gccacctcgc
    60061 aacaatgtca tcgcgttaat ccgtcgcgcc aggcgcgaga agatccatga agcaatgata
    60121 agtccgatgc cgttaaggcc aaacaccagg ctaaattgca ttggactaaa gccaaattcc
    60181 ttctgcaaca cgaacgacga agagccgata tatgcaaaca aaccagccag cataaaactt
    60241 tgaatcaggc aaaagcgtat aaaacggcgg ttctgcacca cgcttcgtcc ggttaatagc
    60301 aatgatgagc cccttttatt ttctggcaag gtctcattaa taaataacag gcagcccagc
    60361 aacagtacgg tgctaatttc agccattacc cagaataaag tgcgccagtc aaaagtgcta
    60421 acaatgtacc cgcccagcac tggcgacaac accggtgcca ggccattcac cgtcattaac
    60481 agcgcaaaaa actgggtcaa cgttactccc tgatatttgt cacgagcaat agaacgagag
    60541 agcaccgaac cacccgcccc cgcgatccct tgaataaaac gccagaccac cagccagtaa
    60601 atattgttcg ttgtcgcgca taaaatggaa gaaacaataa acaatagcaa cgacaagatt
    60661 aacggacgtt tacgccctat tttgtcgctc agagggccaa ataacagttg tccgacgcca
    60721 agaccaatca gtgatgcggt cagggttaat tgcgttatgg tcgtggttgc cgccagttgc
    60781 tcagacatct ccggcaaagc cggcaaataa agatcggtac acatcgggcc gataccggct
    60841 aaaagaccaa gaatcaatgc ccatgaaaga gagactctag ccatattatt ccttacttga
    60901 gtgatgacat accgccgccc agcgactgcc agagcgtgat tcggtttgta aaatcagtca
    60961 gttgcagtgc cagcagctcc tgctgtgcgc tccacaagct gcgctgcgcc gtcaacacgg
    61021 tcaggtaatc accaacaccc gcctgatagc gacgcaagcc aacatcgacc gtttgttgct
    61081 cagctttaac atactggcgc tgtgcatcca gttgctcttc aagcgtggtc cgccgcgcca
    61141 gtgcgttagc gacatcttta aatgcgctct gaacggtttt ttcataggtg gcaatgagcc
    61201 cacgcttttg tgcttctgcg taacgaagct gcgccagatt acttccgcca gtgaacagcg
    61261 gtaatgtgac cgatggcgca aatgaccaga tctgcattcc gtggctgaac aacgacgaca
    61321 atgcgtcact gccaacaccg gcactggcag tcagcgaaat cgtcgggaaa aagttagccc
    61381 gcgccgcgcc aatatctgca ttggcgctct tcaaattatg ttcagcttcc tggatatccg
    61441 ggcgacgcag cagaacatca gatgatactc ccgcaggaac cagacttatc atttgttccg
    61501 gcaggctttc cagtgttccc ggtagcagat tttccgcaag cgttgtacct gccagtaagt
    61561 tcagcgcatt tttatcctgc atcacttgtg tctgatagct ggcaacgctg gcgcgcgctt
    61621 gctgataaac actcatcgcc tcactgacat ctgtcgctgc cgccgtgcca atctgttgct
    61681 ggcgctggat aattttcaat gagttttctg cactggtcat cgtttctttt gccagggcca
    61741 ggttgctgtt atcagccgcc agtgtcagcc aggcggtgct gatttcagca atcagcgtta
    61801 aacgagtgtt ctgggctgta aattcactgg caagccaggt ttcctgcgcc gcccgagata
    61861 aactctgatt acgaccaaac agatctaacg tataactgga caccgtgccg tcagcctcgg
    61921 cagttgttcc cgtaccattc gccagcgaac gactgcgagt cgaacttaag gccgcattca
    61981 ccgtcggaaa taacgacgca ttagtttgcc catagagcgc ccgtgctgag tcaatatcag
    62041 caatcgcttt ttgcacatcg cggttactgt tcagtgcaat ggttactacc tgctgcaatc
    62101 ggggatcgtg gatcacctgc tgccagtcat ggctgattgc cttcccctgg ccctgtgctc
    62161 ccggcaaggt cgccgggatt ggggattcag gcgtactgta gtggggatcg agcgagacac
    62221 atcccgcact caacaacacc agaaaaataa gagaacgacg caacataact tactcctgac
    62281 ggcgcggttt accggcaaac aaacgtttca ccagtacaaa aaacagagga acaaaaaaaa
    62341 tagccagtaa tgtagcggtc agcgtgccgc caataatgcc cgtaccaatg gcgatgcggc
    62401 tgttcgcccc tgcaccggtt gctatcgcca gcggcattac ccccgcaata aacgccagcg
    62461 aggtcatgat gattgggcgt aaacgagtct gcgccgcgcg taatgccgca cgactcaagg
    62521 aatagccctc tgcaaccgcc gcttcagcaa attccacaat cagaatggcg tttttcgacg
    62581 acaagccgat agtggttaat agcgccacct ggaagtaaac atcgttgttt aaatcacgca
    62641 tccaggccgc cagcgccgcg ccaagaagcc ccagagggat caccagaatg accgagaacg
    62701 ggactgacca gctttcgtac agtgctgcaa ggcagaggaa aaccaccaga atagaaactg
    62761 catacagact caatgcctga ccgctggcca gtttctcctg caatgacaaa ccactccagg
    62821 cccaggttgt tcccgcagga agactgtttg ccagttcctc catttttgtc attgcatcgc
    62881 cggaactaaa gccagtcgca ttttccccct gaatttcata ggctgcagaa ccgttgtagc
    62941 gcaccagtct ttccggtcca tacagccagc gggtggtcgc aaaagcagag aatggtgtca
    63001 tggcgttatc gctaccgcgc acaaaccatt tacctaagtc tgacggcgcg gaacgaaatt
    63061 cactgtcgcc ctgaatgtag acttttttca cacgaccacg gtcaataaag tcattcacat
    63121 aagtaccgcc ccacgcactg gacagggtgt cggtgacatc attcaaactc aaccctaatg
    63181 acaccgcttt attactatca atatctacct gcaattgcgg catttgtggc aaatcattgg
    63241 cacgcacaga atgtaactcc ggactttgat tcgctttttc tatcaactga ttacgcattt
    63301 gcagtagtgt ttcgcgatcg gttccaccgt tagctaacaa ttcaaacgta aaaccattgc
    63361 tttggcccag cccatcaacg gctggcggcg tcatcgcaaa taccgtggca tcacgaattg
    63421 tgcccagctc tttggtagcc cgtagggcga tagcctgggc ggtgttttct gcccctttac
    63481 gttgagacca gtttttcaac gaaacaaacg ccatcccggt gttctgtccg ctgccgctaa
    63541 aactgaaacc atcaacggta aaaatgacat cggtatttgc tttctcgtta atcaggaacc
    63601 agtcaacaat ctggcgattc acttctgctg tacgggcagc ggtcgccccg gccggcagcg
    63661 tgtactgcac catgatttcg ccctggtctt cagtgggtaa gaaactgccc ggcagtttcc
    63721 acatcatcag ggccattcca ccacccagta cgacataaag ccccatcgtt cgagcagcac
    63781 ggcgcaggac ataaattacg cctcgctgat atttatcttc agtacggcgg taaaagcggt
    63841 taaatgcgcc gaaaaagcct tttttatgtg gcggaacatg ctggaggacg gaaccgcaca
    63901 gggcgggagt caaggtcaat gccaccacca cggaaagcag cattgcggag atgatggtga
    63961 tggagaactg acgataaatt acgcccgtag atcccccaaa gaaggccatc ggcaggaata
    64021 cggctgacaa caccagcgca atggcaacca gtgcgccaga aatctcgccc attgattttc
    64081 cgtggcttca cgcgcgggca acccttcgtc acgcataatg cgttcgacgt tctctaccac
    64141 cacaatggca tcgtccacca gcaagccgat cgccagcacc attgcaaaca acgtcagggt
    64201 attaatcgaa tagccaaaca acgcaagaac gccgaatgtg cccagcaaaa cgacggggac
    64261 agctaacgcc ggaatcagtg tggcacgcag gttttgcagg aataaataca tcacgcaaac
    64321 caccaatacg atggcttcaa acagcgtctg aattacgtcc tctacggaga ttttaataaa
    64381 ttcagtgctg tctttcgggt atgcgatgtc gtatccctgc ggcatgttcc gctggaattc
    64441 ggcaatctta tccttgacca gcgtcgccgt attcagcgca ttcgcaccgg gcgaaagcat
    64501 tacagcaacc ccggcagacg ggtgaccgtt aagtttgccg atagcggtat aatcttcact
    64561 gcccatctca acgcgagcca catcttttat acgaacaact gcgccgtctg actggctttt
    64621 cacgataata tttttgaact gatccaccgt ctgcaaacga gactgcgcac gtaccgttgc
    64681 ggtcagttgc tgagtattcg gtgaaggcaa tgcccctatt ttcccggcag taacctgcac
    64741 gttttgcgct tcaatagcac tttgcacgtc tgaaggcatt agcgagtaag acgccagttt
    64801 ggccgggtca agccagatgc gcatagcgta ttccgcgcca aagacttgca gactccccac
    64861 accttcaaca cgcgccagcg ggtcctgaac gttactaacc aaccagtcgg cgatatccga
    64921 actggaggct ttgtcagtgg tgtcatacac ggcagcaatc agcaaaaagt tgctttgtga
    64981 tttctccacc gtaacacccg tttgctgcac ttcgctgggt aggcgcgact ccgcctgctg
    65041 aattttattc tgcacctgaa cctgtgcagt atctggatcg gtaccttgtt caaaggtcac
    65101 attaatactg accgaaccat cagagctgct ggttgagctg aaatagagta aattatcaag
    65161 cccagtgagt tgttgctcga taacctgagt cacgctgttc tccagcgttt cggcagaagc
    65221 accagtataa gtggctgaaa ttttaatggt cggtggcgca acgtcaggat attgcgcgac
    65281 gggcaatgtg cgaatagcca gaatccccgc caacataatc aaaatggcga taacccaggc
    65341 aaagaccggg cggcgaacaa aaaagcgcga aaacatcagg cgttgcctcc attagcctga
    65401 acttcaacag ccttgacggt ctgccctgaa gtgactttgg cagaaccttc aacaatcagt
    65461 cggtcgccgt tgtgcaggcc gttcagcacc agccatttat caccatacgt ttctcccgtt
    65521 tcgagcgttc gctgctctac tttattgtct ttattcacca ccagcgcagt tgcattgcct
    65581 ttagcatcgc gcgtgacgcc ctgttgcggc gcgagaatag cgtcttccat cacgccttca
    65641 tcgacacgag cgcgaacaaa cattcccggt aataactgct gttgtggatt gggaaaaatc
    65701 gcccgtaatg tcaccgaacc ggtagactca tctacagcga cttcggtgag ttccagacgc
    65761 cctttttcgc tgtaagttgt gccatcttcc agaataagtg agacgctcat ggtgtcactg
    65821 ttggtcgcca gtgactgttt acgcagacgt aataaatcga cactagagcg agtgaggtcg
    65881 acatacattg tatccagacc acgaatagtc gttaacgctg tatcctgcga cgcggtcacc
    65941 agtgcgccag gggttaccga cgaaatacca atacgccccg aaatcggtgc ggttaccgtg
    66001 gtccagtcaa gattaatgcg cgcagtttcc agtgcggctt ttttcgcctc tacactggct
    66061 ttatcttgtg cacaggtaga ctgagcatca tcagcatcct gttgtgaaac accgttctct
    66121 ttcaccagtc gggcataacg ctgcgctttc tggcaatcgg cttttaccag tgcctgtgct
    66181 tgttgtaatg ctgcccgagc ttcattccat gcagcctgat aactggccgc atcaatctgg
    66241 tagagcggct gtccagcctt gaccagatca ccttccttaa ataagcgttt ctggataatt
    66301 cccccaacct gcggacgtac ttcagcactg agcgcagcac tggtgcgtcc ggttaattca
    66361 ctgactaccg aaaccggttg agatttgagc gtaacaacgc cgacttctgt ttctgacggg
    66421 gatgaattgt tactttgcgt attatcacaa cccgataaga gcagcattgc cactacagat
    66481 gttgctatat acttcattat tttctccagg gtgagaaata ccctcctcat tgaaattaat
    66541 gaggatatgt cagggatatt cagttataac gaacccgcat cgcgaaatga atttcgcaat
    66601 gaaataaggg ggattaatat cccccgctat tggttaattc tgttcttatt tatttatcaa
    66661 catactaaca atatcctgat atatttcctg cagatgttgt ggatcgcttt tttgcggtgt
    66721 taaacgacga taaaccgtac cttccatcat ggtagcaaac acttcaacgc agcaacgaat
    66781 gtgctcgtcg ctaagatggg gatgcattct tttcatatgt gcacaaccgt tagcgaacat
    66841 tctggcgtca gcttcttcaa gcattgccat aacccgcggg ttgcgtgtgg cttctgcagc
    66901 cacttccagc atcagcgcgt cgtcgtcctc atttaaggat ttacgcaagg caaggacttc
    66961 cgggaagtga tcggtacgcg cgtcaatgtc catttgggca atacgaaagt cgatgatacg
    67021 gcgaaccatc tcttcaataa tggcgtcttt gttggcgaag taacgatata tctgacccac
    67081 gctaagctga gcttcgctgg caatttgcga catactggcg gcatgaaatc cgcagcgacg
    67141 aaaacaccga cgggcggcgt taatgatttc atcctgacgc tgtttgtggc gtatctccag
    67201 cttgctggtc atttctgtct cctggcctat cacttgatga gaatgatcgt tctcattttt
    67261 gctaattata cgtttctcca aagtaatgga aagtattttt atcttattca tgatgcatat
    67321 tctgataatg aagagattgt ggagattctg tgaagatagt tgacaatatt aagttggggt
    67381 caattcatca gaaagacagc gacttgttgt cgccagttct gttttcatca tcaaatttat
    67441 cggagctaat acgaatagtt tcatagtcag attctataag atatttcccc aacctccccc
    67501 ttgcaggcgt caggagcaga taacgcaaaa tgctctgtac cgcagcttct ctccgctaca
    67561 gaaagcgcaa ctatagccac ccacagccag gttgattata ataaccgttt atctgttcgt
    67621 actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg
    67681 attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa
    67741 ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc
    67801 acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct
    67861 gacatcgttc tgcagtgcga tgttgccgaa gataccagca tcgacaccat gttcgctgaa
    67921 ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc
    67981 gatcagctgg atggtgacta tgttaacgcc gttactcgtg aaggcttcaa aattgcccac
    68041 gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg
    68101 ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac
    68161 gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg
    68221 ggtccggaag gtgtgcgtgt taacgccatc tctgcgggtc cgatccgtac tctggcggct
    68281 tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc
    68341 cgtaccgtta ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc cgatctctct
    68401 gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac
    68461 gaactcgaac tgaaataatc gttctgttgg tcaagacggg cggcgttctg ccgcccgtta
    68521 tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct
    68581 tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaaaaa cgccacatta
    68641 ccggcaaaag ctactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc
    68701 ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg
    68761 aagaaacgct ggcccccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggaat
    68821 tgttccctga ccagcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt
    68881 cgacggccct gacggttgct caggtttgcg gcgtccaacg gttatgtaac tactattcgg
    68941 cgcgacttac gccgctcccc gggcctgatt cctccaggga aagtaatcat cggttagcac
    69001 aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc
    69061 gccagcatct gaatgacgtc ggcctaactg cctgggactg tgtaatcatt aaccaaatca
    69121 ttggttttat tggctttcag gcgcggtcaa ttgcgacatt tcaggcttat ctcgggcatc
    69181 cggtacgctg gttacccggt ctggagatac aaaactatgc cgacgcgtca ctgtttgctg
    69241 atgaatcaat acgctggcga agcagctatg aggtggaaaa actccctgaa gagcacacaa
    69301 aaagttcaac tgcagaactt tgccaactgg ccgaaacact ctctctccac cctatttcac
    69361 tttcccttct cgaaaagttg ctaaacagca cacgggtcaa tacacagccg gataatcagc
    69421 ttgcggcgtt gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc acctgtatga
    69481 attcatcaaa tgaatataaa aaaatcagcg cccttctgcg caagggcgaa aatgaaatta
    69541 accaatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga
    69601 cccgagcacc cgatcgcttt agcgccgccc agttcagccc attactcgaa cacgaaaaat
    69661 catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct
    69721 taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac
    69781 tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta
    69841 gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc
    69901 acagaaaaag gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg
    69961 ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggttatcca cagtgaaaaa
    70021 gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt ttctgactcg tttcgtgggt
    70081 aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact cttaaaagac
    70141 gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg
    70201 gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca
    70261 caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct tgcgcgccat
    70321 aatctggaga aagaagcacc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt
    70381 cgtgaagatc tgaccgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg
    70441 gatgacgccc ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat tgtggcgatt
    70501 gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc
    70561 gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac
    70621 gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc
    70681 tccgctgatg gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa
    70741 gcgaagctgg tgtatgacct ggtttctgac tggctggaaa ataccggtga ctggaagcct
    70801 gaaagtgaag caattgctga acaagtccgt ttgttagcgc aaatttgcca acgccgcggc
    70861 gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc
    70921 ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc
    70981 gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc
    71041 ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgttggca
    71101 gcgttgctga aaacgcacgg tctgcatgtc gatgctgaag aagtgctcac gctggacggt
    71161 ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt
    71221 cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt
    71281 ctggaggcat acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac
    71341 cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga tgaaatcact
    71401 gtccaaatgg ccgagcgtcg ccgtctcaac cgaatggcag aacgtgatgt tggcgactgg
    71461 ttatacgcac gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc ggcggaaatt
    71521 gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc tatcgccttt
    71581 attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc
    71641 actgtacaaa ttaaaggtga aacggcttac aaagtgactg acgttattga cgtcaccatt
    71701 gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc
    71761 acggcccatt ccttatgaat gggccgttta tttccccgct ctaccttcat catatcccgg
    71821 cagtttttta actgactttc gtttgaaaac tggcgttttt ccaaaactgg attacaaata
    71881 aatatataaa aaaaacaatg cgtttagccg ccgttaattt catgtaagtc atgttgttcc
    71941 ttttatttaa ccgcggactc cgctgttaac cggaggatat gcatcatgaa aaccgttagg
    72001 gagtccacaa cgttgtacaa ctttctcgga tcgcacaatc catactggcg gttaacggaa
    72061 agcagcgatg ttttgcgctt ttctaccacc gaaaccacag aacctgaacg tacgttgcag
    72121 ttatctgccg aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg
    72181 atgagtctga ccgtcgatga aagcgatctt tctgtgcatc tggtaggacg aaaaatcaat
    72241 aaacgggaat gggctggcaa cgcgtctgca tggcatgaca caccagcagt tgctcgtgat
    72301 ttatcacacg gactttcctt tgctgagcag gtagtttctg aagcacattc cgcaatagtg
    72361 attctcgaca gccgggggaa tatccaacgc ttcaatcggt tatgtgaaga ttacacaggg
    72421 ttgaaagaac atgacgtcat tgggcaaagc gtgtttaaac tgtttatgag ccgtcgtgaa
    72481 gctgcggcat ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc
    72541 gaactgtgga taccaacacg taaaggccag cggctgtttc tgtttcgcaa taaatttgtc
    72601 cacagcggca gtggcaagaa cgaaattttt ttaatctgtt ccggcaccga cattaccgaa
    72661 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    72721 ccgaatcgta acgcaatgca ggagttaatc gatcacgcca ttaatcaggc agataacaat
    72781 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcttatggg
    72841 catttgtttg gtgaccagct attacgcgac gtgtcattgg ccattttaag ctgtctcgaa
    72901 catgatcagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    72961 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg actccccttc
    73021 cgcattggtt taattgaagt ttataccagc tgttcagtgg gtatttcact ctctcccgaa
    73081 catggttcgg acagcgcggc tattattcgt cacgccgaca cagcaatgta cacagcgaag
    73141 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    73201 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    73261 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    73321 cagtcacctg aacgtggatt gattccaccg ttggacttca tttcctacgc cgaagagtca
    73381 gggctaattg tgcctttagg ccgttgggtg attctcgatg tagtacgcca ggtggcaaag
    73441 tggcgggata aaggtattaa cctgcgagtg gcggtaaaca tttctgcacg tcagctcgcc
    73501 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    73561 cctattgatg ttgaactgac ggagagctgc ctgattgaga atgatgagct ggcactgtct
    73621 gttattcaac aatttagcca actaggtgcg caagtgcatc tggacgattt tggtaccggt
    73681 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    73741 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    73801 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    73861 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    73921 cccgccgtcg ccttcgaacg ttggtataaa cgctatctga agcgcgcata agtagatgaa
    73981 tatatatact tagcgatata tttcgtaatt atatatcgta ttcccggatg ataattagcc
    74041 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tcccactttt
    74101 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    74161 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    74221 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    74281 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    74341 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    74401 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    74461 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    74521 tccgccgtgc acatggcttt gcagtatcgc ttgatagtga tgacgtcgaa acccgtatga
    74581 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    74641 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctgggcg
    74701 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    74761 acgcgccttg tgaaattatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    74821 ttggcccgtt gacacgccag tgtatccaac aggtacattt cagcaaagca tttattggta
    74881 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    74941 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    75001 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    75061 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgttg
    75121 ttgacattta accatttccc ttctctacgg atgatttgca ctttggcaaa tcatccgctc
    75181 taagatgatt cctggttgat aattaagact atttacctgt tattaacact ctcaagatat
    75241 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    75301 gcgagcggat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    75361 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    75421 atgaccgcgg ctgttctggc aattaccctg gcaatgtctc tgagtgcctg ttctaactgg
    75481 tctaaacggg atcgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    75541 ctgaccgatg gcagtacgtt gggtacatta ggtggcgcag ctgtcggtgg tgttattggt
    75601 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    75661 ttgattctgt tcagtacgaa ataactgaat aatacacaca gtaaaatatt cgaaagccac
    75721 ggatatatcc gtggcttttt atgttaacca ccggcgagtt ttaccttcat ccctttcgct
    75781 tcaagcagtg attttaataa atcacgctta tcgccctgga tttcaataat tccatcttta
    75841 actgccccgc cgcagccgca ttttttcttc agttccgctg ccagttttgt cagttcggca
    75901 tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    75961 cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt tggctcatca
    76021 atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    76081 gctgtaaaga ggcgttgatc gctttcagcg tttgcgctgg atctaccgat tgcgttaccg
    76141 ggcgaccaat caccatataa tcaacaccag ccgccaacgc ctgttctggc gtcataatgc
    76201 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact
    76261 cctgaccaaa tacctgttta aagcgcgcag cttcctgagc agaacacacc acaccatcaa
    76321 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    76381 cgccaagatc ggccaggtcg ctggcttcca tgctggtcaa tactgtcaca gcaatcaaaa
    76441 gcggtgcatc tttgccaaac ggcaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    76501 caccggaggc gtgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    76561 gcgctgcagt gttgggaata tcgtgaaatt tcaggtcgag gaagatatca aaaccacgct
    76621 gttgaagttc gcgtacaaat tgaggtccaa acagcgtaaa catctctttg ccgactttca
    76681 gacgacaatc gcgtgggtcg atcttgtcga caaaggccag cgcggcatca cgattatgat
    76741 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    76801 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    76861 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttctcccc tggcaggctt
    76921 ttctgcgccg gagttaccgg agcacaatga acatcaatta ttagtatgtt gtaactaaag
    76981 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat ggttgaccag
    77041 gcccgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag
    77101 cgataacgag gcttactacg caccttctcg ccaaccatgt cacgcagcac catcagactc
    77161 tctttggcac gcccttcttc cgcttcattt aagtggtaat ccattagctt atggaacaca
    77221 cgcatggtcg gatgacgctg aagctgacgc gtaatataga cctgtgcggc ctcactaccg
    77281 tcgcgcgctt cgataatatc cgcaagcatc aattcagcat cggcaccggt gttctcttcc
    77341 accgcgcgct gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    77401 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gagatatcac gcgttgcaga
    77461 ctttcgacgg ctttggcgta ttctcctttc gccataaata cgcgtcccat catgatggat
    77521 acgcgggcgc tgtttttatc tgccgccgca ccttttttta acaaggccat ggcgcgatcg
    77581 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    77641 cgctgcttat ctttacccaa cttcaccaga cgttcggcaa catcaattgc tttctgccac
    77701 tcgctggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    77761 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    77821 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    77881 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    77941 cctagcgtaa ggtgggcttc aacggtaccc gtatcctctt taagcatatc gagaaacagg
    78001 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    78061 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    78121 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    78181 ttcgccgctg acgaatgcgg cgctacagcc acgtcagtcg cgggtgaaag ctggttttcc
    78241 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacaga
    78301 ccgcaaatca gccaaccgat agcaaacccc gcagcaaaca ataccgccag caatgtggaa
    78361 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    78421 cctaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    78481 acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc
    78541 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gatgatgatt
    78601 taagggctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gcgctatttc
    78661 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    78721 caccgccacc agcgcccacg aaatcaacgt agctaccacc agatcgcgtg gccaatgcat
    78781 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga tagcaatggt
    78841 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agcgcccaac tggcagcaaa
    78901 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    78961 ttgtgggata tttttctctt cagccaactg ttctttcacc agatttccgc gttctgctcg
    79021 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccagcc agataacaaa
    79081 aggtcgtggt tcctggactt tgtctttgat ccaggattta acgccttgtc ccacaagaat
    79141 tgccgccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    79201 gagaaaccag ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    79261 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccaacc
    79321 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtct
    79381 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaacgcc
    79441 agttccagga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    79501 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    79561 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    79621 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    79681 tatctccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    79741 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcactga cgcaaattgc
    79801 cgaagaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    79861 gaataaaatc cgcgcttatg cactgcagga tcaaggttac gatactgtag aggctaacca
    79921 ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    79981 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    80041 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    80101 cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc
    80161 ttgcattgtg taattcattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt
    80221 acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    80281 ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca tccgcgcgcg
    80341 taagcgtcac cggaaccgtc gcgccgggtt gtaggttttg cagatcgcca atatcaatct
    80401 tctcttcccc ggttagccct aacgttttac gcgttacgcc ttgcggaaat tccagcggca
    80461 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    80521 cacgaatacc aagcagacgc ggaccttttg ccgcccagtc acggctggag cctgatccat
    80581 actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc atcgcggcat
    80641 catagataga gacatcatcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    80701 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    80761 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    80821 atcgacccgc cgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    80881 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccaca ggtgctggtg
    80941 ttgcctgcat ttcatcaaag aatggcgata agcgaatata ggttgaatct tcctgccaac
    81001 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    81061 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    81121 gtgccgaggg ccagatatct ttcagatata ccgggtcgcc tttacgatcg tgaccaatag
    81181 gctcagaagc gaggttaata ttcatatttc ccgccagcgc ataggcaacc accagcggcg
    81241 gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    81301 cggacagcac cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    81361 gcagcggccc agagttacca atacaggtgg tacaaccgta ccccacaagg ttaaacccca
    81421 gttcgtcgag atacggtgtc aatttcgctt ttgccagata gtcagaaaca actttcgagc
    81481 ctggtgccag cgacgctttg acccacggtt gccgcttgag gcccagagtt acagcttttt
    81541 tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    81601 tcgcagcaat gaccaccgcg ccatcaggta actgatactg atgtccgttc ataacataat
    81661 cgaccggctg gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg
    81721 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg tttaggccct gccaggctcg
    81781 cttcaacgtc attcatatcc agttctaacg tactggtaaa aattggttca tcgcccgggt
    81841 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga tcttcgctgc
    81901 gcccgcttaa acgcatgtaa tcgagggtga cagcatcgat tgggaagaag ccacaggtgg
    81961 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccagcggt agtgaatcca
    82021 gcccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg
    82081 taacggtgag aaccaggtct gtggcggtaa taccttcacg taattttccg gtgagtttaa
    82141 agcccactac gtccgggata agcatggaaa ccggctggcc taacattgcg gcttcggctt
    82201 cgatgccacc aacgccccac cccagcacgc caaggccgtt gatcatggtg gtgtgcgagt
    82261 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcgctccaca
    82321 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    82381 cgctaaaacg actgaacgcc tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    82441 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    82501 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    82561 caccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    82621 taaagtcctg catcagcacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    82681 ttttcagcca tcccgccagc gcgtggatat cctcttcggt aaccgagtta ccatcctgcc
    82741 agcgcagcag gttttcgagc aaaactttga gtgacttagg cagacgggtg atattgccca
    82801 gtgatttggc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    82861 tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    82921 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    82981 ataacaaccc aaattgataa aagaggagaa accttgagac aaagcaaaca gcggggtcag
    83041 ggatcagtga gtaatgatcc agaggagttc ggcccagaag agcatcgaca gagaaaatgc
    83101 gcccatccag gaccaatatt tcatatttgt gttgatcata ggctacacct ttaacggtta
    83161 ttactttgtg ttgagataac tcaacctgtt attgctgcgg tgtattaaaa gccctttaat
    83221 tcattaaaaa tgggtaataa gaatgatgat aattattgtt tctgctgatc atcttcagca
    83281 aacagttcta taaaggcttg ctgctgcgat gttagttgcc agtcgtctgg aataacgacg
    83341 gaacattccc gtttcgtttc tgtttcattg tcactatgct gacataatgg tgacgactcc
    83401 agttgctcct gaccgaccat agttatcccc acactttcca aatcagcagt gcgacaacca
    83461 cccagaaaag agcagaacca agaaataccg ccagccaggc tttgcgtttt aattcagggt
    83521 cccggcgggg ttcctgattt ccagaaggca ttgctaacct catataatcg ccatcactta
    83581 tcagcaagac gttgaacgat tggtgtaatt aatcatacga tgagacaaat cctaaagaaa
    83641 ctttagtcaa aaatccagcg aatttacgaa tcgtctccag tgaataattc aatcttttga
    83701 cctgaaatag atatttgaaa actcgagttt gcgaggcggg taattagaca ctactttaca
    83761 ggtaatttac cctataaata tgagggataa aaatattgtg cacggtatac aaataagcaa
    83821 caaaagagaa tatattccgg caccttcgct acataaaagg tgccgaaaat aacgcaagaa
    83881 attatttttc cggcagtttt atatctttaa acatgatctc aatttcttca ttagagcgta
    83941 atgcgacagc cgcatcaacg acatcacgcg ttaaatgcgg tgcaaaacgc tgaatgaaat
    84001 catacatata actgcgcaaa aaagtactac ggcgaaaacc aattttggtt gtactgtggc
    84061 tgaagatatc gtgagcatca acgcgcacca ggtcgggatc ggcgaccgga tccaccgcca
    84121 tgctggcaat gacccctacc cccagcccta accggacgta agttttaatg acgtcagcat
    84181 ccgttgccgt gaagacgata cgcggcgtta accctgcgcg gttaaaggca gtatccagct
    84241 ctgaacgtcc ggtaaagccg aaggtatatg tcaccaacgg atattgcgcc agttcttcaa
    84301 tggtaatggc ttttttgcct gccagcgggt gatccggagt gactacaata gcccgattcc
    84361 agtggtagca cggtaacatc actagatctt catacagatg caacgcttct gtggcgatag
    84421 cgaaatcagc attgccttta gagacggcat cagcaatttg tgtcggcgag ccctggtgca
    84481 tatgcaaaga aacgcgggga taacgctcaa taaagccttt gatgacgttt ggtaacgcgt
    84541 agcgtgcctg ggtatgcgtg gtggcgatat acagcgagcc tttatccggc caggtgtgct
    84601 ctccggcgac cgattttatg gcatcaactt tcgacaggac ttcgcgagcg atacgaatta
    84661 tttcttgccc tgctggcgtt acctgcgtca ggtgcttgcc gcttcgggaa aaaatttgaa
    84721 tgcctagctc gtcttccagc attctgactt gtttactgat cccgggttgt gatgtgtaaa
    84781 gtccttccgc tgttgatgag acattcaggt tatgattgac cacctcaaca atatagcgaa
    84841 gttgttgtaa tttcatgtta aaccatccac ttagacgcca tcagaacatc gttttttcgt
    84901 cacacctggc gggtgactaa tacgatttaa taataaaaaa agtgctaact ataac
//
LOCUS       DAZGTJ010000008        84835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000008 DAZGTJ010000000
VERSION     DAZGTJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.008"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..820
                     /locus_tag="ACHK8E_000750"
     CDS             <1..820
                     /locus_tag="ACHK8E_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343057.1"
                     /translation="LKNILDVMIEEDDKGKACFTVNRIRSQDDRVTFIIEQKIKPSES
                     LLDKPWSLLEPKSFLTEKKHDSIDKNDASVSVKKVGLSEGDALKNTGVDKQEDDYSRH
                     VGTEESKSKVSGTEQNKNKGKTPPKVDRNVLVPSYVKFNFRGHKKCSRIFNELKSHLT
                     FDNTPNAISILLRIFIDLSVSTFVEENKLQFKEPHRTPGLHDKVKMCANFLRENKKMT
                     GSQCTAVITFSSQITKHDGSLQQYVHNPHLIPSKEAVNTEWDNFELLLSLIWSE"
     gene            890..1774
                     /locus_tag="ACHK8E_000751"
     CDS             890..1774
                     /locus_tag="ACHK8E_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP0343058.1"
                     /translation="MKFYTPLRYPGGKGKLSYFLKDVIEKNSLNDGAYAEPYAGGAGV
                     ALELLLEEYVRKIYINDADFAVYSFWSSVVSDTDELCRLISRAKIDMDEWSFHRYVIS
                     NPSEFTKLEVGFAAFFLNRTNRSGILKAGVIGGKSQNGKWKMDVRFNRADLISRIEKI
                     ANYNQRIVVTNLDALDFLDALNFMDNMGKVDKNKILLYLDPPYYIKGQGLYRNFYEHD
                     DHVLVMKKLTEINFPNWLVSYDNAKEIKDIYNGFPQVEYSLQYTAHLKKSGEEVMIFS
                     PEIIIPNTLKGVSMPTCA"
     gene            complement(2110..3144)
                     /locus_tag="ACHK8E_000752"
     CDS             complement(2110..3144)
                     /locus_tag="ACHK8E_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0343059.1"
                     /translation="MSKKKGKTPRPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
     gene            complement(3144..4916)
                     /locus_tag="ACHK8E_000753"
     CDS             complement(3144..4916)
                     /locus_tag="ACHK8E_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP0343060.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            5090..5944
                     /locus_tag="ACHK8E_000754"
     CDS             5090..5944
                     /locus_tag="ACHK8E_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0343061.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHDAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            6003..7076
                     /locus_tag="ACHK8E_000755"
     CDS             6003..7076
                     /locus_tag="ACHK8E_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0343062.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            7080..7823
                     /locus_tag="ACHK8E_000756"
     CDS             7080..7823
                     /locus_tag="ACHK8E_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP0343063.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDIRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRVPRKTRSVTPAKRGRPKKKAS"
     gene            7923..8432
                     /locus_tag="ACHK8E_000757"
     CDS             7923..8432
                     /locus_tag="ACHK8E_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0343064.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            8432..8635
                     /locus_tag="ACHK8E_000758"
     CDS             8432..8635
                     /locus_tag="ACHK8E_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0343065.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            8639..8920
                     /locus_tag="ACHK8E_000759"
     CDS             8639..8920
                     /locus_tag="ACHK8E_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0343066.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            8920..9417
                     /locus_tag="ACHK8E_000760"
     CDS             8920..9417
                     /locus_tag="ACHK8E_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP0343067.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            9432..9857
                     /locus_tag="ACHK8E_000761"
     CDS             9432..9857
                     /locus_tag="ACHK8E_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP0343068.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLELYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            9845..10270
                     /gene="lysB"
                     /locus_tag="ACHK8E_000762"
     CDS             9845..10270
                     /gene="lysB"
                     /locus_tag="ACHK8E_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP0343069.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            10170..10415
                     /gene="lysC"
                     /locus_tag="ACHK8E_000763"
     CDS             10170..10415
                     /gene="lysC"
                     /locus_tag="ACHK8E_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP0343070.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            10378..10845
                     /locus_tag="ACHK8E_000764"
     CDS             10378..10845
                     /locus_tag="ACHK8E_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0343071.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSMSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            10838..11290
                     /locus_tag="ACHK8E_000765"
     CDS             10838..11290
                     /locus_tag="ACHK8E_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0343072.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEETILAHLER"
     gene            complement(11362..12147)
                     /locus_tag="ACHK8E_000766"
     CDS             complement(11362..12147)
                     /locus_tag="ACHK8E_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343073.1"
                     /translation="MFSDNVINAWWFISLYLFLLIALTFVTFGKSNLMRFIAHHFNLE
                     YSDRKLKMLDKKWRDIQLFKTINGINVSGIEDVRMIQQGLIDGKLKTSYFFLTRFWGD
                     ITKPPHIIKTIIVILASIIYILFACYIHNKQSAIVRDAIGIPYKNMMYYVYSDKVLLS
                     FKNKTVELNKTYSLADCKTLQNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKDNNSH
                     KKALLILSLVYFISGLLIFLSYTKFLYANKKVLEYKASNKNHS"
     gene            12231..12866
                     /locus_tag="ACHK8E_000767"
     CDS             12231..12866
                     /locus_tag="ACHK8E_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0343074.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITSTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            12863..13210
                     /locus_tag="ACHK8E_000768"
     CDS             12863..13210
                     /locus_tag="ACHK8E_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0343075.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            13215..14123
                     /locus_tag="ACHK8E_000769"
     CDS             13215..14123
                     /locus_tag="ACHK8E_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP0343076.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            14116..14646
                     /locus_tag="ACHK8E_000770"
     CDS             14116..14646
                     /locus_tag="ACHK8E_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0343077.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDSCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            14657..16702
                     /locus_tag="ACHK8E_000771"
     CDS             14657..16702
                     /locus_tag="ACHK8E_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0343078.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSMSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQ
                     IANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAV
                     QKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKW
                     RSRQSTTTKDLMNLKWDALYVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEENLY
                     IIPTRENEGENGDIGSLRPFGINLRTGVVSVGNGARIDGGLALGTNNALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDI
                     RLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            16706..17233
                     /locus_tag="ACHK8E_000772"
     CDS             16706..17233
                     /locus_tag="ACHK8E_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0343079.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQEQQAESQKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            17469..18053
                     /locus_tag="ACHK8E_000773"
     CDS             17469..18053
                     /locus_tag="ACHK8E_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0343080.1"
                     /translation="MDKKFKNQIVDSDIARLLLKHRKARNLTVTELAQRSGVSQAMIS
                     KIERGISSPSATILSRLANAMNVTLSKLFAELEQHQNSLVRFAHQQQHWTDEQSGITR
                     WSLSPAGACPELIKVEIPAMGELTIPASANEHLSGQTLWMMSGSLDFRVKNQTHHLRA
                     GDCLALTFASEYQMSNPDSEQSCSYIVAFSQKNQ"
     gene            complement(18074..18517)
                     /locus_tag="ACHK8E_000774"
     CDS             complement(18074..18517)
                     /locus_tag="ACHK8E_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0343081.1"
                     /translation="MLDIRMSRPSEASEIIQIWKSSVDATHDFLTGHDRQEIEKEVIG
                     FFSETPVWVATNQDDQPLGFMFLHEGHLEALFVAGSARGLGVGKRLISHALALHPELS
                     VDVNEQNQQAVGFYQHMGFQVSGRSDLDNQGRPYPLLHLRMANNM"
     gene            18930..20120
                     /locus_tag="ACHK8E_000775"
     CDS             18930..20120
                     /locus_tag="ACHK8E_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP0343082.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            20133..20651
                     /locus_tag="ACHK8E_000776"
     CDS             20133..20651
                     /locus_tag="ACHK8E_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0343083.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            20708..20983
                     /locus_tag="ACHK8E_000777"
     CDS             20708..20983
                     /locus_tag="ACHK8E_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0343084.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            20980..21135
                     /locus_tag="ACHK8E_000778"
     CDS             20980..21135
                     /locus_tag="ACHK8E_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0343085.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            21128..23575
                     /locus_tag="ACHK8E_000779"
     CDS             21128..23575
                     /locus_tag="ACHK8E_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0343086.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAETMAGVKLLMSGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTVASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            23590..24069
                     /locus_tag="ACHK8E_000780"
     CDS             23590..24069
                     /locus_tag="ACHK8E_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0343087.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            24069..25232
                     /locus_tag="ACHK8E_000781"
     CDS             24069..25232
                     /locus_tag="ACHK8E_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0343088.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDISGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            25314..25532
                     /gene="ogrK"
                     /locus_tag="ACHK8E_000782"
     CDS             25314..25532
                     /gene="ogrK"
                     /locus_tag="ACHK8E_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFP0343089.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(25805..27166)
                     /gene="yegQ"
                     /locus_tag="ACHK8E_000783"
     CDS             complement(25805..27166)
                     /gene="yegQ"
                     /locus_tag="ACHK8E_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP0343090.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(27314..27646)
                     /gene="yegP"
                     /locus_tag="ACHK8E_000784"
     CDS             complement(27314..27646)
                     /gene="yegP"
                     /locus_tag="ACHK8E_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP0343091.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(27837..28559)
                     /gene="baeR"
                     /locus_tag="ACHK8E_000785"
     CDS             complement(27837..28559)
                     /gene="baeR"
                     /locus_tag="ACHK8E_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP0343092.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(28556..29959)
                     /gene="baeS"
                     /locus_tag="ACHK8E_000786"
     CDS             complement(28556..29959)
                     /gene="baeS"
                     /locus_tag="ACHK8E_000786"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP0343093.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(29956..31371)
                     /gene="mdtD"
                     /locus_tag="ACHK8E_000787"
     CDS             complement(29956..31371)
                     /gene="mdtD"
                     /locus_tag="ACHK8E_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343094.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            complement(31372..34449)
                     /gene="mdtC"
                     /locus_tag="ACHK8E_000788"
     CDS             complement(31372..34449)
                     /gene="mdtC"
                     /locus_tag="ACHK8E_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP0343095.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            complement(34450..37572)
                     /gene="mdtB"
                     /locus_tag="ACHK8E_000789"
     CDS             complement(34450..37572)
                     /gene="mdtB"
                     /locus_tag="ACHK8E_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP0343096.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(37572..38819)
                     /gene="mdtA"
                     /locus_tag="ACHK8E_000790"
     CDS             complement(37572..38819)
                     /gene="mdtA"
                     /locus_tag="ACHK8E_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP0343097.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            39098..39154
                     /locus_tag="ACHK8E_000791"
     CDS             39098..39154
                     /locus_tag="ACHK8E_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0343098.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            39426..39482
                     /locus_tag="ACHK8E_000792"
     CDS             39426..39482
                     /locus_tag="ACHK8E_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0343099.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            39754..39810
                     /locus_tag="ACHK8E_000793"
     CDS             39754..39810
                     /locus_tag="ACHK8E_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0343100.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            40083..40142
                     /gene="ibsA"
                     /locus_tag="ACHK8E_000794"
     CDS             40083..40142
                     /gene="ibsA"
                     /locus_tag="ACHK8E_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP0343101.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            40363..41022
                     /gene="yegL"
                     /locus_tag="ACHK8E_000795"
     CDS             40363..41022
                     /gene="yegL"
                     /locus_tag="ACHK8E_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0343102.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            41019..41780
                     /gene="pphC"
                     /locus_tag="ACHK8E_000796"
     CDS             41019..41780
                     /gene="pphC"
                     /locus_tag="ACHK8E_000796"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFP0343103.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            41777..43716
                     /gene="yegI"
                     /locus_tag="ACHK8E_000797"
                     /pseudo
     CDS             41777..43716
                     /gene="yegI"
                     /locus_tag="ACHK8E_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(43729..45081)
                     /gene="yegD"
                     /locus_tag="ACHK8E_000798"
     CDS             complement(43729..45081)
                     /gene="yegD"
                     /locus_tag="ACHK8E_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0343104.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            45215..46063
                     /gene="alkA"
                     /locus_tag="ACHK8E_000799"
     CDS             45215..46063
                     /gene="alkA"
                     /locus_tag="ACHK8E_000799"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP0343105.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(46172..49489)
                     /gene="dgcE"
                     /locus_tag="ACHK8E_000800"
     CDS             complement(46172..49489)
                     /gene="dgcE"
                     /locus_tag="ACHK8E_000800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0343106.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            49807..50448
                     /gene="udk"
                     /locus_tag="ACHK8E_000801"
     CDS             49807..50448
                     /gene="udk"
                     /locus_tag="ACHK8E_000801"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP0343107.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            50540..51121
                     /gene="dcd"
                     /locus_tag="ACHK8E_000802"
     CDS             50540..51121
                     /gene="dcd"
                     /locus_tag="ACHK8E_000802"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP0343108.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            51143..52996
                     /gene="asmA"
                     /locus_tag="ACHK8E_000803"
     CDS             51143..52996
                     /gene="asmA"
                     /locus_tag="ACHK8E_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP0343109.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(53049..53339)
                     /locus_tag="ACHK8E_000804"
     CDS             complement(53049..53339)
                     /locus_tag="ACHK8E_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0343110.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(53329..53667)
                     /locus_tag="ACHK8E_000805"
     CDS             complement(53329..53667)
                     /locus_tag="ACHK8E_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0343111.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYK
                     ELVQERKNEN"
     gene            complement(53742..55325)
                     /gene="yegH"
                     /locus_tag="ACHK8E_000806"
     CDS             complement(53742..55325)
                     /gene="yegH"
                     /locus_tag="ACHK8E_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0343112.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(55526..55675)
                     /locus_tag="ACHK8E_000807"
     CDS             complement(55526..55675)
                     /locus_tag="ACHK8E_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343113.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            55984..57123
                     /gene="wza"
                     /locus_tag="ACHK8E_000808"
     CDS             55984..57123
                     /gene="wza"
                     /locus_tag="ACHK8E_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP0343114.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            57129..57572
                     /gene="wzb"
                     /locus_tag="ACHK8E_000809"
     CDS             57129..57572
                     /gene="wzb"
                     /locus_tag="ACHK8E_000809"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP0343115.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            57575..59737
                     /gene="wzc"
                     /locus_tag="ACHK8E_000810"
     CDS             57575..59737
                     /gene="wzc"
                     /locus_tag="ACHK8E_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP0343116.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            59915..60754
                     /gene="wcaA"
                     /locus_tag="ACHK8E_000811"
     CDS             59915..60754
                     /gene="wcaA"
                     /locus_tag="ACHK8E_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP0343117.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            60757..61245
                     /gene="wcaB"
                     /locus_tag="ACHK8E_000812"
     CDS             60757..61245
                     /gene="wcaB"
                     /locus_tag="ACHK8E_000812"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP0343118.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            61242..62459
                     /gene="wcaC"
                     /locus_tag="ACHK8E_000813"
     CDS             61242..62459
                     /gene="wcaC"
                     /locus_tag="ACHK8E_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP0343119.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            62434..63651
                     /gene="wcaD"
                     /locus_tag="ACHK8E_000814"
     CDS             62434..63651
                     /gene="wcaD"
                     /locus_tag="ACHK8E_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP0343120.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            63662..64408
                     /gene="wcaE"
                     /locus_tag="ACHK8E_000815"
     CDS             63662..64408
                     /gene="wcaE"
                     /locus_tag="ACHK8E_000815"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP0343121.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            64424..64972
                     /gene="wcaF"
                     /locus_tag="ACHK8E_000816"
     CDS             64424..64972
                     /gene="wcaF"
                     /locus_tag="ACHK8E_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP0343122.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            64999..66120
                     /gene="gmd"
                     /locus_tag="ACHK8E_000817"
     CDS             64999..66120
                     /gene="gmd"
                     /locus_tag="ACHK8E_000817"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0343123.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            66123..67088
                     /gene="fcl"
                     /locus_tag="ACHK8E_000818"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             66123..67088
                     /gene="fcl"
                     /locus_tag="ACHK8E_000818"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0343124.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            67091..67570
                     /gene="gmm"
                     /locus_tag="ACHK8E_000819"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             67091..67570
                     /gene="gmm"
                     /locus_tag="ACHK8E_000819"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0343125.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            67567..68790
                     /gene="wcaI"
                     /locus_tag="ACHK8E_000820"
     CDS             67567..68790
                     /gene="wcaI"
                     /locus_tag="ACHK8E_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP0343126.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            68793..70229
                     /gene="cpsB"
                     /locus_tag="ACHK8E_000821"
     CDS             68793..70229
                     /gene="cpsB"
                     /locus_tag="ACHK8E_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP0343127.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            70422..71792
                     /gene="cpsG"
                     /locus_tag="ACHK8E_000822"
                     /gene_synonym="manB"
     CDS             70422..71792
                     /gene="cpsG"
                     /locus_tag="ACHK8E_000822"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0343128.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            71847..73241
                     /gene="wcaJ"
                     /locus_tag="ACHK8E_000823"
     CDS             71847..73241
                     /gene="wcaJ"
                     /locus_tag="ACHK8E_000823"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP0343129.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            73243..74721
                     /gene="wzxC"
                     /locus_tag="ACHK8E_000824"
     CDS             73243..74721
                     /gene="wzxC"
                     /locus_tag="ACHK8E_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP0343130.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            75382..76662
                     /gene="wcaK"
                     /locus_tag="ACHK8E_000825"
     CDS             75382..76662
                     /gene="wcaK"
                     /locus_tag="ACHK8E_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP0343131.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            76659..77879
                     /gene="wcaL"
                     /locus_tag="ACHK8E_000826"
     CDS             76659..77879
                     /gene="wcaL"
                     /locus_tag="ACHK8E_000826"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP0343132.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            77890..79284
                     /gene="wcaM"
                     /locus_tag="ACHK8E_000827"
     CDS             77890..79284
                     /gene="wcaM"
                     /locus_tag="ACHK8E_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP0343133.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            79442..80437
                     /locus_tag="ACHK8E_000828"
     CDS             79442..80437
                     /locus_tag="ACHK8E_000828"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP0343134.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            80680..81573
                     /gene="galF"
                     /locus_tag="ACHK8E_000829"
     CDS             80680..81573
                     /gene="galF"
                     /locus_tag="ACHK8E_000829"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP0343135.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            81945..83030
                     /gene="rfbB"
                     /locus_tag="ACHK8E_000830"
     CDS             81945..83030
                     /gene="rfbB"
                     /locus_tag="ACHK8E_000830"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0343136.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            83030..83929
                     /gene="rfbD"
                     /locus_tag="ACHK8E_000831"
     CDS             83030..83929
                     /gene="rfbD"
                     /locus_tag="ACHK8E_000831"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP0343137.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            83987..>84835
                     /gene="rfbA"
                     /locus_tag="ACHK8E_000832"
     CDS             83987..>84835
                     /gene="rfbA"
                     /locus_tag="ACHK8E_000832"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0343138.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQ"
BASE COUNT        20760 a        21380 c        22903 g        19792 t
ORIGIN      
        1 attaaaaaat attttagatg taatgattga agaggatgat aaaggtaagg cttgttttac
       61 tgttaatagg attcgtagcc aagatgacag ggttacattt attattgaac aaaaaataaa
      121 accttcagaa tctcttttag ataagccgtg gagtttactg gagcctaaat cttttcttac
      181 agagaaaaaa catgattcta ttgacaaaaa tgatgcatct gtgagtgtaa agaaagtagg
      241 tctttcagaa ggtgatgcct taaagaatac aggtgtggat aaacaggaag atgattattc
      301 tagacatgta ggcacagaag agagtaaatc taaagtttcg ggtacggaac agaataaaaa
      361 caagggaaaa actcctccta aagtagatcg taatgtgttg gtgccatctt atgtgaaatt
      421 taattttaga gggcataaga aatgctctag aatatttaat gagcttaagt cgcatctgac
      481 atttgataac acccctaatg ctatctcaat attgttgaga atatttatag atctctctgt
      541 aagcactttt gttgaagaaa acaagttaca atttaaggaa ccacatagaa ctccagggct
      601 tcatgataag gttaaaatgt gtgctaattt ccttcgggaa aataaaaaga tgacggggag
      661 tcaatgtacg gctgtaataa ctttctcgag ccaaataact aaacatgatg gttcattgca
      721 gcagtatgta cataacccac acttaattcc ctcaaaagag gctgtcaaca ctgagtggga
      781 taactttgag ttgttattaa gccttatttg gagtgagtga gatttgtcta catctggaca
      841 tggatgtcca gaaactgata aactacaaaa tcaggaatga gagggattta tgaaatttta
      901 tactccatta agataccccg gcgggaaagg taagttatct tactttttaa aagatgttat
      961 tgaaaaaaac tcgttgaatg atggagcata tgcagaacct tacgctggtg gtgcaggggt
     1021 ggctttagaa ttattattgg aagagtatgt ccgtaaaatc tatataaatg atgcagattt
     1081 tgctgtttat tctttttggt catctgttgt tagtgatact gatgagcttt gccgacttat
     1141 tagccgagca aagatagata tggatgaatg gtcttttcat cgctatgtta tttcgaatcc
     1201 ttctgagttt actaagcttg aggttggctt tgctgcgttt ttcttaaata gaacaaatcg
     1261 ttcaggtatt ctcaaggcgg gggttatagg cgggaaatca caaaatggaa aatggaaaat
     1321 ggatgttcgc tttaacaggg cggatttaat ttcaagaatt gagaagatag caaattataa
     1381 tcaaagaata gtagtaacta atctggatgc tttggatttt cttgatgcct tgaattttat
     1441 ggataatatg ggaaaggtgg ataaaaataa aattcttttg tatttggatc ctccttacta
     1501 tattaaaggg caagggcttt acaggaattt ttatgagcat gatgaccatg tattggttat
     1561 gaagaaactc acagaaatta atttcccaaa ttggcttgtt tcttatgata acgctaagga
     1621 aattaaagat atttataatg gatttccgca agttgagtat tcattgcaat atacagcgca
     1681 tctcaaaaaa tctggagagg aagtgatgat tttttctcct gaaataatta ttcctaatac
     1741 cctgaaaggg gtctcaatgc caacttgcgc ctgattttac ttattttttg tcagtaagta
     1801 attagtttgg tgcatttatt tgcattcatt tttaaatgcc agttctggta tctaacatca
     1861 gtgctggcgc attctaacca ttactgctgc actgcattta aagccctgca tgaagcgggc
     1921 gggcgaggcg gggaaagcac tgcgcgctgg cggtggtgct gattttattt tttcagcgtc
     1981 tgagcgcgtc gtgacggcgc ttagtctgcc cgttgaggcg ttggtgtgtc tgcggggtgt
     2041 tttgtgcggt ggtgagcgtg tgagggcgtg atgacggggt gtaaaaaagc cgcccgcagg
     2101 cggcgatgtt cagtcgttgt cagtgtccag tgagtagttt ttaaagcgga tgacctcctg
     2161 accgagccag ccgtttatct cgcggatcct gtcctgtaac gggataagct cattgcggac
     2221 aaagaccttt gccactttct caatatcacc cagcgacccg acgttctccg gcttgccgcc
     2281 catcaactga aaggggatgc ggtgcgcgtc cagcaggtca gcggcgctgg cttttttgat
     2341 attaaaaaaa tcgtccttcg ttgccacttc actgagcggg ataattttaa tgccgtcggc
     2401 tttcccctgt ggggcataga gaaacagatt tttaaagtta ttgcggcctt tcgacttcac
     2461 catgttttcg cgaagcattt cgatatcgtt gcgatcctgc acggcatcgg tgacgtacat
     2521 gatgtatccg gcatgcgcgc cgttttcgta atacttgcgg cggaacagcg tggccgactc
     2581 attcagccag gcagagttaa gggcgctgag atattccggc aggccgtaca gctcctgatt
     2641 aatatccggc tccagcaggt gaaacacgga gccgggcgcg aaaggtgtcg gctcgttgaa
     2701 ggacggcacc caccagtaaa catcctcctc aacaccacgg cgggtatatt ttgccggtga
     2761 tgtttccagt ctgatgacct taccggtggt gctgtagcgc ttttccagaa acgcattacc
     2821 gaacaccaga aaatccagca caaagcggct gaaatcctgt tgcgaaagcc acggatgcgg
     2881 gataaatgtt gaggccagaa tattgcgttt gacgtaaatc ggtgagctgt gatgcacggc
     2941 agcacgcagg ctttttgcca gaccggtaaa gctgaccggt ggctcatacc atctgccgtt
     3001 actgatgcac tcgacgtaat ccagaatgtc acggcggtcg agtaccggca ccggctcacc
     3061 aaaggtgaat gcctccattt tcggggcgct ggcggtcatt gtttttgccg ctggtcgcgg
     3121 tgttttccct tttttcttgc tcatcagtaa aactccagaa tggtggatgt cagcggggtg
     3181 ctgataccgg cggtgagtgg ctcatttaac agggcgtgca tggtcgccca ggcgaggtcg
     3241 gcgtggctgg cttcctcgct gcggctggcc tcataggtgg cgctgcgtcc gctgctggtc
     3301 atggtcttgc ggatagccat aaacgagctg gtgatgtcgg tggcgctgac gtcatattcc
     3361 agacagccac ggcggatgac gtcttttgcc ttgagcacca ttgcggtttt catttccggc
     3421 gtgtagcgga tgtcgcgtgc ggcgggatag aacgagcgca cgagctggaa cacgccgaca
     3481 ccgaggccgg tggcatcaat accgatgtat tcgacgttgt atttctcggt gagtttgcgg
     3541 atggattcag cctgagtggc aaagtccatg cctttccact ggtgacgctc aagtattctg
     3601 aatttgccac cggccaccac cggcggtgcc agtaccacgc atccggcgct gtcgccacgg
     3661 tgtgacgggt cgtaaccaat ccataccggg cgggagccga acggattcgc ggcaaacggc
     3721 gcatagtctt cccattcttc cagcgtgtcg accatgcagc gttgcagctc ctcgaacggg
     3781 aacaccgacg ccttgtcgtc aacaaattca cacatgaaca ggtttttaaa atcgtcggcg
     3841 ctgttttcgc gtttgagctg ctcaatgtcg aacagcgtgc agccgccttt cagggcgtcc
     3901 tcaatggtga caatctgccg ccactggccg tccgcacaga gaagtccacc ggcaagggcg
     3961 ttatgactga cgtcgatttc cacgcgttcg gcggcgctgg cgcgtccccg gttaaacagt
     4021 tcacccgacc agaacgggta ggcgtcgtgc gccagcgtgg acggggtgga gaaataggtc
     4081 gaacgcaggt gactctgtga ggccatacct gatgccacct tacgcagtac ctgaaaattc
     4141 gggatccaga aaatctcgtc gacgtacagg tcgccgttat ggctctgtgc ggtgttggag
     4201 ttggtgccga gaaaaatcag ttttgcgccg ttattgccca ggacaatcgg gtcaccggtc
     4261 aggtcaacgt caaccagccg ggcaaaggcg atgatgtatt cgcggaacac atacgcctgc
     4321 gttttactgg ccgacagaaa aatctggtta tgaccggttt tcagggcgcg cagcagcgcc
     4381 tcgcgggaaa aataaaacgt cgcgccaatc tggcgggatt tcaggatatc gcggatgcgg
     4441 tgctcaagcc cggcgcgata ccagtgcaac tgatagtcga aagactgctc aaagaaaatc
     4501 tgctccagct tttcgatggc ttcgtcactg aaaaaattct ttttcggttt gcgacgccca
     4561 cctttgttgc ggttagcaat gttcggatta aggtctgcct cgttgccggt ctggctgtaa
     4621 cggttgaccc gtgccagtcg ttcaatctgg cgtcccagca ggtcaatttc cttgaagtcc
     4681 ccgccggttt tctgcggttt gatgatgagc tgggtcagcc gcgcttccag actcatttcg
     4741 acacggctga tgggggcaac gctgtcccag ccgtcgcgct gtttccagct ctgcaccgtc
     4801 gggcgtttca tctgcaacat ggcggcaatc tgcggcacgg aaaatccctg ccagtacagc
     4861 agcgccgcct gacgacgcgg gtcgtgtaaa agagtggtgt ctgtggtgat ggtcatgaat
     4921 acctcgccgt gataaataca cggcaaggct actgagtcgc gtcccgcgat tcgctaaggt
     4981 gctgttgtgt cagtgataag ccatccggga ctgatggcgg aggatgcgca tcgtcgggaa
     5041 actgatgccg acatgtgact cctctaatca ctattcagga ctcctgacaa tggcaaaaaa
     5101 agtctcaaaa ttctttcgta tcggcgttga gggtgacacc tgtgacgggc gtgtcatcag
     5161 tgcgcaggat attcaggaaa tggccgaaac ctttgacccg cgtgtctatg gttgccgtat
     5221 taacctggaa catctgcgcg gcatcctgcc tgacggtatt tttaaacgtt atggtgatgt
     5281 ggtcgaactg aaggccgaaa agattgacga tgattcggcg ctgaaaggca aatgggcgct
     5341 gtttgcgaaa atcaccccga ccgatgacct tatcgcgatg aacaaggccg cgcagaaggt
     5401 ctatacctca atggaaattc agccgaactt tgccaacacc ggcaaatgtt atctggtggg
     5461 gctggccgtc accgatgacc cggcaagcct cggcacggaa tacctggaat tctgccgcac
     5521 ggcaaaacac aaccctctga accgcttcaa attaagccct gaaaacctga tttcagtggc
     5581 aacgcctgtt gagctggaat ttgaagacct gcctgaaacc gtgttcaccg ccctgaccga
     5641 aaaggtgaag tccatttttg gccgcaaaca ggccagcgat gatgcccgtc tgaatgacgt
     5701 gcatgacgcg gtgaccgctg ttgctgaaca tgtgcaggaa aaactgagcg ccactgagca
     5761 gcgcctcgct gagatggaaa ccgccttttc cgcacttaag caggaggtga ctgacagggc
     5821 ggatgaaacc agccaggcat tcacccgcct gaaaaacagt ctcgaccaca ccgaaagtct
     5881 gacccagcag cgccgcagca aggccaccgg tggtggcggt gacgccctga tgacgaactg
     5941 ctgaccggcg tcagtcagtc cgggaaaacc ttcacgatta acccttaatt tcaggaaaaa
     6001 ctatgcgcca ggaaacccgc tttaaattta atgcctacct gtcccgtgtt gccgaactga
     6061 acggcatcga cgccggtgat gtgtcgaaaa aattcaccgt tgaaccgtcg gtcacccaga
     6121 ccctgatgaa caccatgcag gagtcctctg actttctgac ccgcatcaac attgtgccgg
     6181 tcagcgaaat gaaaggggaa aaaattggca tcggtgtcac cggctccatt gccagcacca
     6241 ccgacaccgc cggtggcacc gagcgtcagc cgaaggactt ctcgaagctg gcgtccaaca
     6301 agtacgaatg cgaccagatt aacttcgatt tttatatccg ctacaaaacg ctggacctgt
     6361 gggcgcgtta tcaggatttc cagctccgta tccgtaacgc cattatcaaa cgccagtccc
     6421 ttgatttcat catggccggt tttaacggcg tgaagcgtgc cgaaacctct gaccgcagca
     6481 gcaatccgat gctgcaggat gtggcagtcg gctggctgca gaaataccgc aatgaagctc
     6541 cggcgcgcgt gatgagcaag gtcactgacg aggaagggca caccacctct gaggtcatcc
     6601 gcgtgggcaa gggcggtgat tatgccagcc ttgacgcact ggtgatggat gcgaccaata
     6661 acctgattga gccgtggtat caggaagacc ctgaccttgt ggtgattgtg ggacgtcagt
     6721 tactggcgga caagtatttc cccatcgtta acaaggagca ggacaacagc gaaatgctgg
     6781 ccgctgacgt catcatcagc cagaaacgca tcggtaacct gccggcggta cgcgtcccgt
     6841 acttcccggc ggatgcgatg ctcatcacga agctggaaaa cctgtccatc tactacatgg
     6901 atgacagcca tcgccgcgtg attgtggaaa acccgaaact cgaccgcgtg gagaactacg
     6961 agtcaatgaa cattgattac gtagtggaag actacgccgc cggttgtctg gtggaaaaaa
     7021 ttaaggtcgg tgatttctcc acaccggcta aggcgaccgc agagccggga gcgtaaccga
     7081 tgacgagtcc cgcacagcgc cacatgatgc gggtctcggc agcgatgacc gcgcagcggg
     7141 aagccgcccc gctgcgacat gcaactgtct atgagcagat gctggttaag ctggccgcag
     7201 accagcgcac actgaaagcg atttattcaa aagagcttaa ggccgcgaaa aagcgcgagc
     7261 tgctgccgtt ctggttgccg tgggtgaacg gtgtgctgga gcagggcaaa ggtgcacagg
     7321 atgacattct gatgacggtc atgctgtggc gtctggatac cggcgatatt gccggtgcgc
     7381 tggagattgc ccgttatgcc ctgaagtacg gtctgaccat gccgggtaaa caccgccgca
     7441 ccccgccgta catgttcacc gaggaggtcg cactcgcggc catgcgcgct cacgctgccg
     7501 gtgaatctgt ggatatccgc ctgctgacgg agacccttga actgaccgcc acggctgaca
     7561 tgcctgatga agtgcgcgca aagctgcaca aaatcaccgg tctgtttctg cgtgatggtg
     7621 gtgatgccgc cggtgcgctg gctcacctgc aacgtgcgac acagctcgac tgtcaggcag
     7681 gcgtcaaaaa agagattgaa cgactggagc gggagctgaa accgaagccg gagccgcagc
     7741 ccaaagcggc cacccgtgtc ccgcgtaaga cccggagcgt gacaccggca aaacgtggac
     7801 gcccgaaaaa gaaagccagt taacaaccga atgcgccccg cgccagggcg gcacgccggt
     7861 cagtgacggt gaattacctg acactgcacc ggcgtccacc gcccgacttt tcagaggtag
     7921 tcatgatgac gctgattatt ccgcgaaagg aggctcccgt gtccggtgag ggtacggtgg
     7981 tcatcccgca accggcaggc gacgagccgg tgattaaaaa cacgttcttt tttcccgata
     8041 tcgacccgaa gcgcgtccgg gaacgtatgc gccttgagca gaccgtcgcc cccgcccgtc
     8101 tgcgtgaggc catcaagtca ggcatggctg aaacgaatgc ggagctgtac gagtaccgcg
     8161 aacagaaaat tgccgccggt tttacgcgtc tggcggacgt tccggcggac gacatcgacg
     8221 gtgaaagcat caaagttttt tactacgagc gcgccgtgtg tgcgatggcg accgcgtcgc
     8281 tttatgagcg ttatcgcggc gtggatgcca gtgcgaaagg cgacaagaag gccgacagca
     8341 ttgacagcac cattgatgaa ctgtggcggg atatgcgctg ggcggtggcg cgtatccagg
     8401 acaagccgcg ctgcatcgtg agtcaaatct gatgaagacc tttgcgctac agggcgacac
     8461 gctcgacgcc atttgtgtcc ggtattacgg gcgcactgag ggcgtggttg agaccgtgct
     8521 cgccgcaaat ccgggactgg ctgaactggg tgcggtgctg ccacacggca ccgccgtcga
     8581 actgcccgac gttcagaccg cgcccgtggc tgaaactgtc aatctgtggg agtaacgcat
     8641 gacagcagaa gaaaaaagcg tcctgtcgct tttcatgatt ggggtgctga ttgttgtcgg
     8701 caaggtgctt gccggtggtg aacccatcac cccgcgtctg tttatcgggc gcatgttgct
     8761 cggcggtttt gtctcgatgg ttgccggtgt tgttctggtg cagtttcctg acctgtcact
     8821 gcctgcggtg tgcggcatcg gttccatgct gggtatcgcc ggttatcagg tgattgagat
     8881 tgccattcag cgccgcttta agggcagggg gaaaccgtaa tgccggtaat taacacgcat
     8941 cagaatatcg ccgcctttct cgacatgctg gccgtgtccg aagggacggc gaatcatccg
     9001 ctgacgaaaa accggggcta tgacgtgata gtcaccggac tggacggaaa gccggaaatt
     9061 ttcaccgact acagtgatca cccgttcgcg catggccgac cggcgaaggt gtttaaccgt
     9121 cgcggtgaaa aatccacggc ctccggtcgc tatcagcagc tttacctgtt ctggccgcac
     9181 taccgcaaac agcttgccct gccggatttc agtccgttgt cacaggacag gctcgccatt
     9241 cagttgatcc gcgaacgcgg tgcactggat gacatccggg cgggacgcat tgagcgcgcc
     9301 atttcacgct gtcgcaatat ctgggcgtcc ctgccgggtg ccggttacgg tcagcgtgag
     9361 cattcactgg aaaaactggt caccgtctgg cgtaccgccg gcggcgtacc ggcttaaacg
     9421 gagtaaacac catgaagaaa ttatcccttt cactgatgct gaacgtgtcg ctggcgctga
     9481 tgctggcact gtccctgatt tacccgcaga gcgtggccgt cagttttgtc gccacctggg
     9541 cgattctggc gacggttatc tgtgtggttg ccggtggtgt cggcgtgtat gccacggagt
     9601 atgtgctgga actctacggg cgggagctgc cgccggaatc gctggccgtg aagattgtca
     9661 cgtcgctgtt tttgcagccg gtgccgtggc gcagacgggc ggcggctctg gtggtgatgg
     9721 tggcgacgtt tatctcgctg gtcgctgccg ggtggatttt taccgcgctg atttatcttg
     9781 tggcgtcgct gtttttccgg ctgatacgta aagcctgtcg tcagcgtctt gaggggcggg
     9841 aactatgtca aagctgatga ttgtgctggt cgtgttgtta tcgctggcgg tggccggtct
     9901 gtttctggtg aaacacaaaa atgccagcct gcgcgcctcg ctggacaggg cgaataacgt
     9961 cgccagtgaa cagcagacga ccatcaccat gctgaaaaat cagcttcatg ttgccatcac
    10021 cagggcagac aaaaacgagc tggcgcaggt ggcactgcgt caggaactgg agaacgccgc
    10081 gaagcgtgaa gcacagcgcg agaaaaccat cacgaggtta ctcaatgaaa acgaagattt
    10141 tcgccgctgg tacggtgctg acctgcctga tgctgtgcgc cggttgcacc agcgcccggc
    10201 ctgcgcagac gccagtgatt gtccacaacg cctgcccgaa agtgagtctt tgcccgatgc
    10261 cgggcagtga cccgcagaag aacggcgatt taagtgccga tatccggctg cttgagaacg
    10321 cgctggcacg ctgtgccagc caggtaaaaa tgattaaaca ctgtcaggac gaaaacgatg
    10381 ctcaaacccg acagcctgcg cagggcgctg actgatgccg tcacggtgct gaaaaccagt
    10441 cccgagatgc tgcggatatt cgtggataac gggagtattg cctccacact ggcgacgtct
    10501 atgtcattcg aaaagcgtta cacgctcaat gtgattgtga ccgactttac cggtgatttt
    10561 gacctgctca ttgtgccggt gctggcgtgg ctgcgggaaa atcagcccga catcatgacc
    10621 accgacgaag gccagaaaaa gggcttcacg ttttatgcag acatcaacaa tgacagcagc
    10681 tttgatatca gcatcagcct gatgctgacc gagcgcacgc tggtcagtga ggttgacggc
    10741 gcgctgcatg tgaagaatat cccggaacct ccgccgccgg agccggttac ccgcccgatg
    10801 gagctgtata tcaatggcga actggtgagc aagtgggatg aatgagttta agcgttttga
    10861 agaccggctg accggactga ttgagtcgct gtcaccgtca gggcgtcgac gactgagtac
    10921 cgaactggcg aaacgtctgc gtcagagtca gcagcgtcgg gtgatggcac agaaagcccc
    10981 ggacggcaca ccctacgcgc cacgccagca gcagagcgcc agaaaaaaga ccggtcgtgt
    11041 taagcgaaaa atgtttgcga aacttatcac cagtcgcttt ttgcatatcc gcgccagccc
    11101 ggaacaggca tcaatggaat tttacggcgg gaagtcacca aaaatcgcca gcgtgcatca
    11161 gttcggcctg tcggaagaaa accggaaaga cggtaagaaa attgattatc cggcgcgtcc
    11221 cctgctcggc tttaccggtg aggatgtgca gatgattgaa gagactatcc tggctcacct
    11281 tgagcgttag ttttatccag gcagaggctg atgcgcaatt aaacattgag cggccgtgct
    11341 ggtcgctcaa tgtttagagg tttatgagtg gtttttattt gatgctttgt attctaaaac
    11401 cttcttattg gcgtaaagga attttgtata tgacaggaat ataagcaggc ctgaaatgaa
    11461 atagacgagg gatagtatta ataatgcttt tttgtggctg ttattatctt taatctcctg
    11521 acttaaccat tcggagtcct cctcgtttag ctgtaagagc ttattgcagg cgatttcagg
    11581 aagtgtgtct tttataaata cgttttgcag ggtcttgcaa tcggcaaggc tataagtttt
    11641 attaagttca actgttttat ttttgaagga taaaagaact ttgtcactat aaacatagta
    11701 catcatattt ttatatggta tgcctatggc atctcttact atagcggatt gtttgttgtg
    11761 tatataacat gcgaagagaa tataaataat actggccaga attacaatta ttgttttaat
    11821 tatgtgtggt ggttttgtta tgtcacccca gaagcgagta aggaaaaaat acgatgtttt
    11881 tagttttcca tcaatcagcc cctgctgtat cattctcaca tcttcgatgc ctgatacatt
    11941 gattccgtta attgttttaa atagttgaat gtcgcgccat tttttgtcga gcatttttaa
    12001 ctttctgtct gaatactcaa gattgaaatg atgtgcaata aacctcataa gattactttt
    12061 accaaaggta acaaatgtta atgctattaa taaaaataga tacaaagaga taaaccacca
    12121 cgcattaatc acattatcac tgaacattac gctctcctcg aatgttgtat ggtcgttcta
    12181 caaatgaatc cagatagcat aacttttata tattgtgcaa tctcacacgc atgaacactc
    12241 tcgcaaatat tcaggaactc gcgcgcgcac tgcgcaacat gattcgtacc ggccttgtcg
    12301 tcgaaaccga ccttaacgcc ggtcgctgcc gtgtgcagac cggcggcatg tgcaccgact
    12361 ggcttcagtg gctgacccat cgcgccgggc gttcgcgcac atggtgggca ccttccgtgg
    12421 gggaacaggt gctgattctg gccgtgggtg gtgaacttga cacggcgttc gttctgccgg
    12481 ggatttattc cggcgataac cccgcgccgt ctgcgtcggc ggatgccctg catatccgtt
    12541 tccctgacgg ggcggtgatt gagtatgaac ccgaaaccag tgcactcacg gtaagcggaa
    12601 ttaaaacggc cagcgtgacg gcttctgatt ctgttactgc cacggtgccg gtggtcatgg
    12661 tgaaagcgtc aactcgcatc accctggaca caccggaggt ggtctgcacc aacaggctga
    12721 ttactagcac gctggaagta cagaagggcg ggacgatgcg tggcaacatt gaacacaccg
    12781 gcggtgaact ctcatcaaac ggtaaggtac tgcataccca caaacacccc ggcgacagcg
    12841 gcggcacaac cgggagtcct ttatgacagc tcgttatctc ggaatgaatc gcagtaatgg
    12901 cctgactgtc actgaccttg agcatatcag ccagagtatc ggcgatatcc tgcgtacacc
    12961 ggtcggctca cgggtgatgc gtcgtgatta cggctcgttg ctggcatcaa tgattgacca
    13021 gccgcagacc ccggcgcttg agttgcagat taaggtcgcc tgttacatgg cggtgctgaa
    13081 atgggaaccc cgcgtcacgc tgtcatctgt caccacggcg cgcagttttg acgggcgaat
    13141 gacggtcacg ttaaccggcc agcacaacga caccggccag ccactttcgt taaccatccc
    13201 tgtgagttga aaccatgccg attatcgacc tgaaccagct acccgcaccg gatgtggtcg
    13261 aggagctgga ctttgaaacc attctcgccg aacgcaaggc gacactgatt tccctttacc
    13321 cggaagacca gcaggaggcg gtcgcccgta ccctgacgct ggaatctgag cctctcgtca
    13381 aactgctgga ggaaaatgct tatcgtgagc ttctctggcg tcagcgtgtg aatgaggccg
    13441 cacgggcggt gatgctggcc tgtgccgcgg gtaatgacct tgatgtgatt ggtgccaatt
    13501 acaacaccac gcgtctgact atcaccccgg cagatgattc gaccatcccg ccgacaccgg
    13561 ccgtgatgga atctgacacc gattatcgtc tgcgtattca gcaggccttt gaaggtttaa
    13621 gcgtcgccgg gtcggtgggt gcctatcagt atcatggtcg cagtgctgac gggcgtgtcg
    13681 cggatatctc tgtcaccagt ccgtctccgg cctgcgtcac catctctgtg ctgtcacgtg
    13741 aaaataacgg tgtcgcatcc gaagacctgc tggcggtggt gcgtaacgcc cttaatggcg
    13801 aggacgtcag gccggtggcc gaccgcgtga ccgtgcagtc tgccgccatc gttgaatacc
    13861 agataaacgc cacgctttac ctttaccctg gtcccgaaag cgaacccata cgcgctgccg
    13921 ccgtgaaaaa actggaagcg tacatcacgg cacagcaccg gctggggcgc gacatccgtc
    13981 tgtctgccat ttatgccgct ttgcatgtgg aaggcgtgca gcgtgtcgag ctggccgcac
    14041 cactggccga catcgtgctc aacagtacgc aggcgtcttt ctgtaccgaa taccgcgtcg
    14101 tgaccggagg ctcggatgag tgattcgcga ctgttgccga ccggctcatc accgcttgag
    14161 gtcgccgccg caaaagcctg tgcggaaatt gaaaaaacac cggtcagtat tcgtgagctg
    14221 tggaacccgg actcctgtcc ggcaaatctg ctgccgtggc tggcgtgggc gttttcggtc
    14281 gacagatggg atgaaaagtg gccggaagcg acaaaacgcg ctgttatccg cgatgcctat
    14341 ttcatccact gtcataaagg cactataggt gcaatccggc gtgtggtgga gccgctgggc
    14401 tatctcatca acgtgacgga gtggtgggaa accagtgacc cgccgggcac cttccggctt
    14461 gatattggtg tactggaaag tggcatcaca gaggcaatgt atcaggaaat ggaacggctg
    14521 attgctgatg ccaaacctgc aagccgccac cttattggcc tgaacattac ccgggacatt
    14581 cccggctacc tgttcgccgg tggtgtggct tacgacggcg atgtaattac ggtttacccc
    14641 ggataagtga ggaataatga gcacaaaatt caaaaccgtt atcaccactg ccggtgcagc
    14701 aaagctggca gcggcaaccg caccgggagg gcggaaggtc aacattacca cgatggccgt
    14761 cggggatggc ggtggtaaat tgcctgtccc ggatgccgga cagaccgggc ttatccacga
    14821 agtctggcga catacgctga acaaaatcag ccaggacaaa cgaaacagta attatattat
    14881 cgcagagctg gttattccgc cggaggtggg cggtttctgg atgcgtgagc ttggcctgta
    14941 cgatgatgcg ggaacgttaa ttgccgtggc gaacatggcc gaaagttata agccagctct
    15001 tgccgaaggc tcagggcgtt cgcagacctg tcgcatggtc atcatcgtca gcagtgtggc
    15061 ctcggtggag ctgaccattg acaccacaac ggtgatggcg acacaggatt acgttgatga
    15121 caaaattgca gagcacgaac agtcacgacg tcacccggac gcctcgctga ccgccaaagg
    15181 ttttactcag ttaagcagtg cgaccaacag cacgtctgaa acactggccg caacgccgaa
    15241 agcggtaaag gccgcgtatg accttgctaa cgggaaatat accgcacagg acgctaccac
    15301 agcgcgaaaa ggtcttgtcc agctaagtag tgcgaccaac agcatgtctg aaacgctcgc
    15361 cgcaacacca aaagccgtta agacggtaat ggatgaaacg aacaagaaag cgccattaaa
    15421 cagccctgca ctgaccggaa cgccaacgac gccaactgcg cgacagggaa cgaataatac
    15481 tcagatcgca aacacggctt tcgttatggc cgcgattgcc gcccttgtag actcgtcgcc
    15541 tgacgcactg aatacgctga acgagctggc ggcggcgctg ggcaatgacc cgaattttgc
    15601 taccaccatg actaatgcgc ttgcgggtaa gcaaccgaaa gatgccactt tgacggcgct
    15661 ggcggggctt gctactgcgg cagacaggtt tccgtatttt acggggaatg atgttgccag
    15721 cctggcgacc ctgacaaaag tcgggcggga tattctggct aaatcgaccg ttgccgccgt
    15781 tatcgaatat ctcggtttac aggaaacggt aaaccgagcc gggaacgccg tgcaaaaaaa
    15841 tggcgatacc ttgtccggcg ggcttacttt tgaaaacgac tcaatccttg cctggattcg
    15901 aaatactgac tgggcaaaga ttggatttaa aaatgatgcc gacagcgata ctgattcata
    15961 tatgtggttt gaaacaggtg acaacggcaa tgaatatttc aaatggagaa gtcgccagag
    16021 caccacaaca aaagacctga tgaatcttaa atgggatgct ctgtatgttc ttgttaaagc
    16081 ccttttcagt agtgaagtaa aaatatctac agtcaatgcg ctgaggatat ttaattcatc
    16141 ttttggtgct atttttcgcc gttctgaaga aaacctgtat atcattccta cacgagaaaa
    16201 tgagggtgaa aatggagata ttgggtcatt aaggccattc ggcatcaact taagaacagg
    16261 agttgtgtct gttggtaatg gtgccaggat tgatggcggg ctggcacttg gcacgaataa
    16321 cgcgttgggt gggaactcta ttgttcttgg tgataacgac accggattta aacaaaatgg
    16381 cgatggtaat ctggatgttt atgctaataa cgtccatgtt atgcgctttg tttccggaag
    16441 cattcaaagt aataagacca taaatattac ggggcgtgtt aatccctcgg attacggtaa
    16501 ctttgattcc cgctatgtga gagatatcag acttggcaca cgtgttgtcc agaccatgca
    16561 gaaaggggtg atgtatgaga aagcagggca cgtaattacc gggcttggta ttgtcggtga
    16621 agtcgatggt gatgaccccg cagtattcag gccaatacaa aaatacatca atggcacatg
    16681 gtataacgtc gcacaggtgt aatttatgca gcatttaaaa aatattactg cgggtaatcc
    16741 aaaaactgtt gcccaatatc aactgacaaa aaattttgat gttatctggt tatggtccga
    16801 agagggaaaa aactggtatg aggaagtaag taattttcag gaagacacga taaagattgt
    16861 ttacgacgag aataatataa ttgtcggcat caccagagat gcttcaacgc ttaaccctga
    16921 aggtttcagc gttgtcgagg ttcctgatat tacctccaac cgacgtgctg atgactcagg
    16981 taaatggatg tttaaggatg gtgccgtgat taagcggatt tatacggcag atgaacagga
    17041 gcaacaggca gaatcacaaa aggcagcttt actttccgaa gctgaatccg tgattttgcc
    17101 gctggaacgc gctgtcaggc tgaatatggc gacggatgag gaacgcagcc gactggaagc
    17161 atgggaacgc tacagcgttc tggtcagtcg tgtggatcct gcaaatcccg aatggccgga
    17221 aatgccgcaa taagttgtat gatctctggt gtgagcttac atatctatag cacagagtaa
    17281 agcctaatct gacagtccgc tatgtgccaa aagcggacgt taagcttacc aactcatatt
    17341 ttaaggttat aggtgcgtat aaacgaagtg ccgcgcttag gagtagtgaa tttacagact
    17401 tacaaaatca tttatttcag gccaatttat gtaatggcat aatcaggttg gtgaaagaga
    17461 gggcggaaat ggataaaaaa tttaaaaatc agatagtaga tagcgatatt gcacgtttat
    17521 tgcttaaaca caggaaggct cgcaatttga ctgtaacaga gcttgctcaa cgatcgggcg
    17581 tcagtcaggc catgattagt aagattgaac gaggcatctc cagccctagt gcaactatcc
    17641 ttagccgcct ggccaacgcc atgaatgtaa cgctatctaa actctttgca gagcttgagc
    17701 aacatcagaa ttccctggtt cgttttgctc atcagcaaca gcactggacg gatgagcaat
    17761 caggcatcac ccgttggtct ttgtctcctg cgggggcatg cccggaactg atcaaagttg
    17821 aaatccctgc aatgggtgag ctgacgatac ccgcctccgc aaatgagcat ctttccgggc
    17881 aaacgctttg gatgatgagt ggcagccttg attttcgagt caaaaatcag acccatcatt
    17941 tacgtgccgg tgactgtctt gcccttacct ttgcaagcga gtaccaaatg agcaatccag
    18001 atagtgagca gagctgttct tacatcgtgg cttttagcca aaaaaatcaa tgatatcttc
    18061 tctgccacat tggttacatg ttattggcca ttctcagatg caacaagggg tatggccttc
    18121 cctgattatc cagatcggaa cgccctgata cctgaaaacc catatgctga taaaacccta
    18181 cagcctgctg gttttgttca tttacatcaa cgcttagctc tggatgcagc gccagtgcat
    18241 gagatatcaa acgttttccg acgccaagtc cgcgagcaga gcctgctaca aaaagtgcct
    18301 ccagatgccc ctcatgcaaa aacataaatc ctagcggttg gtcatcctga tttgttgcaa
    18361 cccatacagg agtttctgag aaaaagccga taacttcttt ttcgatttct tgtcggtcat
    18421 gacctgtaag aaagtcgtgg gtagcatcca ctgaactctt ccagatttgt atgatctctg
    18481 atgcttcaga tggtcgtgac attctgatat ctaacatttt tcaaccttag agagaacttt
    18541 attttatgta ataacgaacg aaaagagctt actttaattt actatagtga aattatattc
    18601 acaatagtgt ttgcggtttc tatgccttat caacgtgatg agaagtgcta agttagtctg
    18661 atcctcttta tcatcatcaa cgacaatgtt cctgagcttt atcacattga acttccgctt
    18721 ctcgctcaaa gcagactgtc agatttgata gcgttttggc tatgtaaatt gtcagtcggg
    18781 aaataagtga gtacaaatca tgacaggcgg gcggattgtc cgccttttct ttatctgttg
    18841 tttcatccac tgaccagcca ggtcaaatag cgtctcatgc tctgcacaac agaaaatacc
    18901 actcacccat taaccacgga gttagacgga tgagtgacta tcatcacggc gtgcaggtgc
    18961 tggagattaa cgacggcacc cgcgtcattt ccaccgtatc cactgccatt gtcggcatgg
    19021 tctgcacggc cagcgatgcg gatgcggaaa cattccccct caataaacct gtgctgatta
    19081 ccaatgtgca gagcgcaatt gcaaaggccg gtaaaaaagg cacgctggcg gcgtcgttac
    19141 aggccatcgc tgaccagtca aaaccggtca ccgttgttgt gcgcgtggaa gacggcaccg
    19201 gcgacgacga ggaaacgaaa ctcgcgcaga ctgtttccaa tattatcggc accaccgacg
    19261 aaaacggtca gtacaccgga ctgaaagccc tgatgggggc tgagtcggtt accggcgtta
    19321 aaccgcgcat tctcggcgta ccgggactgg acaccaaaga ggtcgccgtc gcactggcat
    19381 cggtatgcca ggaactgaat gccttcgggt atatcagcgc atggggttgt aaaaccattt
    19441 ccgaggtgaa agcctaccgc cagaatttca gccagcgtga gctgatggtt atctggccgg
    19501 atttcctcgc atgggatacg gttaccagta ccaccgccac cgcgtatgcc accgctcgtg
    19561 cgctgggtct gcgcgctaaa atcgaccagg agcagggctg gcataaaacg ctgtccaacg
    19621 tcggggtgaa cggtgttacc ggcatcagtg catccgtctt ctgggatttg cagaagccag
    19681 gcactgatgc tgacctgctg aacgaggcag gcgtcacaac gctggttcgc cgcgacggtt
    19741 tccgtttctg gggtaaccgt acctgctctg atgacccgct gttcctcttt gaaagctaca
    19801 cacgcaccgc gcaggtactg gccgacacga tggctgaggc gcacatgtgg gctattgata
    19861 agcccatcac cgcaacgctg attcgcgaca tcattgacgg catcaatgcc aaattccgtg
    19921 aactgaaaaa caacggttat atcgtggatg gcacatgctg gttcagcgaa gaatccaacg
    19981 atgcggaaac cctcaaggcc ggaaaactgt atatcgacta cgactatacc ccggtgcctc
    20041 ctctcgaaaa cctgaccctg cgccagcgta ttacttccag atacctggca agtctggtca
    20101 cttcggttaa cagcaattaa ggagcctgac cgatggcaat gccgcgcaaa ctcaagttaa
    20161 tgaacgtctt tctgaacggc tacagctatc agggcgttgc aaagtccgtc acgctgccaa
    20221 aactgacccg taagctcgaa aactatcgcg gtgcggggat gaacggcagc gcaccggtag
    20281 acctcggcct tgatgatgat gctctgtcaa tggagtggtc gctcggtgga ttcccggatt
    20341 cggttatctg ggagctttac gctgcaacca gtgctgatgc cgtaccgatt cgttttgctg
    20401 gttcttacca gcgcgacgat accggcgaaa cggtggccgt cgaagtggtc atgcgtgggc
    20461 gtcagaaaga agtcgatacc ggagagggta aacagggaga ggataccgaa tcgaaactcc
    20521 cggtgatttg tacttatttc aagttgacga tggacggtaa ggagctggtc gaaatcgaca
    20581 ccatcaacat gattgagaag gtgaacggcg tcgaccggct ggagcaacac cgccgcaata
    20641 tcggcctgtg attttcatcc ggtcagcctg gctgaccggt taaccccgat tcagaagtga
    20701 gaaaaccatg aacaaagaaa atgtgattac cctggacaat ccggtcaagc gtggtgagca
    20761 ggttatcgaa caggtcacgc tgatgaaacc taatgccggg acgctgcgcg gtgtcagtct
    20821 ggctgcggtc gcaaactccg aagtcgatgc actgattaag gtgctgccgc gcatgacggc
    20881 accgatgctg accgagcagg aggtcgccgc gctggaactg cctgaccttg tggcgctggc
    20941 cggtaaggtg gtcggttttt tgtctccgaa ctcggtgcag tgacgtttcc gaaaaatctg
    21001 tcggtcgatg acctgatggc agatgtggca gtgatatttc actggccgcc atcagaactg
    21061 tatcccatga gcctgaccga actcatcaca tggcgcgaaa aggcgctccg gcgaagcgga
    21121 agcacgaatg agtaacaatg taaaattaca ggtattgctc agggctgttg accaggcatc
    21181 ccgcccgttt aaatccatcc gtacagcgag taagtcgctg tcgggggata tccgggaaac
    21241 acaaaaatca ctgcgcgagc tgaacggtca cgcatcccgt attgagggat ttcgcaagac
    21301 cagcgcacag cttgccgtga ctggtcatgc acttgaaaag gcacggcagg aagccgaagc
    21361 acttgccaca cagtttaaaa acactgaacg tccgacccgt gctcaggcga aagtgctgga
    21421 atccgcaaag cgtgcggcgg aggacttaca ggcgaaatat aaccgcctga cggattccgt
    21481 taagcgccag cagcgggaac tggccgctgt gggaattaat acccgcaatc ttgcacatga
    21541 tgagcaggga ctgaaaaacc gtatcagtga aaccaccgca cagcttaacc ggcagcgtga
    21601 cgcgctggcg cgtgtcagtg cgcaacaggc aaaacttaat gcagtaaaac agcgttatca
    21661 ggccggaaag gaactggccg gaaatatggc ctcagtgggc gctgccggtg tggggattgc
    21721 ggcggcggaa acgatggccg gagttaagtt gctgatgtcc ggttatgagt ttgcgcagaa
    21781 aaactcagaa ttgcaggccg tgctgggtgt ggcaaaagac tccgccgaaa tgaccgcact
    21841 acgcaaacag gcgcgccagc tcggcgacaa taccgccgcc tcggcggatg atgcggccgg
    21901 tgcacagata atcatcgcga aagcgggtgg ggatgttgat gccattcagg cggcaacgcc
    21961 ggtcacgctg aatatggcgc tggcgaaccg ccgcacgatg gaagaaaacg ccgccctgct
    22021 gatggggatg aaatccgcct ttcagctttc aaacgataag gtcgctcata tcggggatgt
    22081 tctctccatg acgatgaaca aaaccgccgc cgattttgac ggcatgagcg atgcgctgac
    22141 ctatgccgca cctgtggcaa aaaatgccgg tgtcagcatt gaagaaaccg ccgcaatggt
    22201 cggggcgctg catgatgcaa aaatcacagg ctcaatggcg gggacgggaa gccgtgccgt
    22261 gttaagccgc ctgcaggcac cgacgggaaa agcatgggat gcactcaaag agcttggagt
    22321 gaaaacctca gacagcaagg gaaacacccg accagtattt accattctga aagaaatgca
    22381 ggccagtttt gagaaaaacc ggctcggtac tgcccagcag gctgaataca tgaaaaccat
    22441 tttcggggag gaggccagct cagccgccgc cgtgctgatg actgtcgcct caaccggaaa
    22501 gctggacaaa ctgaccgctg cgtttaaagc ctcggacggg aagacctcag agctggtaaa
    22561 tattatgcag gacaatctcg gcggtgactt taaggagttt cagtccgctt atgaggcggt
    22621 ggggactgac ctgtttgacc agcaggaagg cgcactgcgt aagctcacgc agacggccac
    22681 aaagtatgtg ttaaaactcg acggctggat acagaaaaac aaatcactgg cgtcaaccat
    22741 cggcatcatt gccggcggtg cactggtgct gactggcatc atcggtgcca ttggccttgt
    22801 agcctggccg gttatcaccg gcatcaatgc cattatcgcg gcagcaggcg caatgggggc
    22861 aatcttcacg acggttggta gtgccgttat gacggccatc ggtgcgatta gctggccggt
    22921 tgtggccgtg gtggctgcca ttgtcgccgg ggcgttgctt atccgtaaat actgggagcc
    22981 tgtcagcgca ttctttggcg gtgtggttga agggctgaaa gcggcatttg cgccggtggg
    23041 ggagctgttc acgccactta agccggtgtt tgactggctg ggcgaaaagt tacaggccgc
    23101 gtggcagtgg tttacaaacc tgattgctcc ggttaaagcc acacaggaca ccctgaaccg
    23161 ttgccgtgac acgggcgtca tgttcgggca ggcactggct gacgcgctga tgctgccgct
    23221 taatgcgttc aacaaactgc gcagcggtat tgactgggta ctggaaaaac tcggtgttat
    23281 caacaaagag tcagacacac ttgaccagac cgccgccaga actcatgccg ccacgtatgg
    23341 caccggtggt tatattccgg cgaccagctc ttatgcaggc tatcaggctt atcagccggt
    23401 cacggcaccg gctggccgct cttatgtgga ccagagtaaa aacgaatatc acatcagcct
    23461 gacgggtggt actgcgccgg ggacacagct tgaccgccag ttacaggatg cgctcgaaaa
    23521 atacgagcgg gataaacgtg cgcgcgcccg tgccagcatg atgcatgacg gttaaggagg
    23581 tgacgaaaaa tgatgctcgc gttaggtatg tttgttttta tgcgccagac gctgccacac
    23641 cagaccatgc agcgtgaatc agattatcgc tggccgtcaa attcccgtat cggtaaacgg
    23701 gacgcctacc agtttctcgg tgtgggtgag gaaaacatca cgcttgccgg tgtgctttat
    23761 ccagaactga ccggcggcaa gctgacgatg accacgctca ggctgatggc agaggaaggc
    23821 cgggcgtggc cgttgctgga tggcaccggc atgatttacg gcatgtatgt catcagcagg
    23881 gtgagtgaaa caggaagtat tttctttgca gacggcacac cccgaaaaat tgattttacg
    23941 ctgtcgctca cccgcgttga tgaatcactg gccgcgcttt atggcgatat cggtaaacag
    24001 gcggaatcgc tcatcggtaa ggctggcagt atggcgacca gattcacggg tatgacgggg
    24061 gcgggataat gctggatgca ctgacatttg atgcaggcag tacgctgacg ccggattaca
    24121 tgctgatgct cgacagcagg gatatttccg gcaatatcag cgaccgactg atgagcatga
    24181 ccctgacgga taaccggggc tttgaggctg accagcttga tattgaactg aacgatgccg
    24241 acgggcaggt cgggctgccg gttcgtggcg ctgtcctgac ggtgtatatc ggctggaaag
    24301 gttttgccct ggtatgcaaa gggaaattta ccgttgatga ggttgaacac cggggcgcac
    24361 cggatgtggt caccatccgc gcccggagtg cagattttcg cgggacgctc aattcccgcc
    24421 gtgaaggctc ctggcatgac accacgctcg gtgcgattgt tgaggcgata gcctcccgta
    24481 acaggctgga agccagtgtc gctccgtcac tggccggaat taaaatcccg cacatcgacc
    24541 agtcgcagga gtctgatgcg aaattcctga cccgtcttgc agaacgcaac ggcggtgagg
    24601 tgtcggtaaa aatgggaaaa ctgttgtttc tcaaagcggg gcagggggtg acggccagcg
    24661 gtaaaaaaat cccgcaggtc accataaccc gcagcgacgg cgaccgccat cattttgcga
    24721 ttgctgaccg tggagcctat accggcgtaa cggcaaagtg gttacacacc aaagacccga
    24781 agccacaaaa gcagaaggta aaactgaaac gcaaaaagaa agagaaacac ctgcgcgcac
    24841 tggagcaccc gaaagcgaaa ccagtcacgc agaagaaagc gccaaaagta ccggaagcgc
    24901 gcgaaggtga atacatggct ggtgaggctg acaacgtttt tgccctgacc acggtatatg
    24961 ccacgaaagc acaggccatg cgcgccgctc aggcgaagtg ggataagctg caacggggcg
    25021 ttgcggagtt ctccatcagt ctggctactg gtcgggcaga tatttacacg gaaacgccgg
    25081 tcaaagtgtc aggctttaag cgcgtcatag acgagcagga ctggacaatc actaaggtga
    25141 cacattttct gaataatagc ggcttcacga cgtccttgga gcttgaggtc aggctttctg
    25201 atgtggagta cgaaacagaa gatgatgagt gatgtttttg ttttatctgt ttgttttata
    25261 aggataaatt aactaaaatg gcaccatcaa caaaaccgga agaggtgctc gcgatgtttc
    25321 attgtccttt atgccagcat gccgcacatg cgcgtacaag tcgctatatc actgacacga
    25381 caaaagagcg ttatcaccag tgccagaacg tgaattgcag cgccacgttc atcacttatg
    25441 agtcggtaca gcgatacatc gtgaagccgg gagaagtcca cgccgtaaga ccgcacccgt
    25501 tgccgtcagg gcagcaaatt atgtggatgt aattacaaac aggaagcccc tcagtcgagg
    25561 ggcttttttg tcgatgtggt caatgtgtgg acgtgaccag aaataaatcc ttttatttca
    25621 ttgtattacg cgtaaaaaat aagcccgtgt aagggagatt acacaggcta aggaggtggt
    25681 tcctggtaca gctagcattt tatgggttat gtttttcagc gaaacggatg ataaccttaa
    25741 taaatgcagc tgtatgtgat cggtttctaa gaatttttca tccgggaaaa ataatcgaaa
    25801 ttaatcactt accgtggggg ttacgcgtgg tttcaccgga gaaattacgc atcagcagcg
    25861 cgtaattcag ttcgagatcc tgcggcaccg ggagccacac agtataacca tcgcctggtg
    25921 cgaccggcat tgcttcacct ttggcgtttt ccatatgctc aagggtaaag ttaatgttgc
    25981 cttgcggcgt cattagctca aggctgtcgc caacggagaa tttatttttc accgctaccg
    26041 ccgcgagctc ccccttgcgc tcaccggtaa actcaccaac aaactgctgg cggtcagaaa
    26101 ccgaataacc gtattcgtag ttctgataat cgtcgtgggt atgacgacgc aggaaacctt
    26161 cggtatagcc acgatgcgcc agaccttcca gcgtttccag caggctggta tcgaacggtt
    26221 tgcccgcagc ggcgtcatcg atagctttac ggtaaacctg cgcggtgcgt gcgcaatagt
    26281 agaaagattt ggtacggcct tcgattttca gcgaatgcac gcccattttg gtcaggcgtt
    26341 caacatgggc gatggcgcgc agatctttcg agttcatgat gtaagtgccg tgctcatctt
    26401 caaacgtggt catatactcg cccggacgct gggcttcttc gatcataaac actttgtcgg
    26461 ttggcgcgcc gatacccagc gtcggctcaa cattttgcac cggaattggc tcgtacttgt
    26521 gtacgatgtt gccaacgtca tcttctttcc cttcctggac gttgtactcc cagcggcagg
    26581 cgttggtgca ggtaccctgg ttcgggtcgc gcttgttgat atagccagag agcaggcagc
    26641 gaccggagta ggccatgcac agcgcaccgt gaacgaagat ctcgatctcc atatccggca
    26701 cctgattgcg gatctcttca atctcttcca gcgacagctc gcgagagagg atcacgcggg
    26761 tcaggcccat ttgctgccag aatttcaccg tcgcccagtt cacggcgtta gcctgtaccg
    26821 agagatggat cggcatttca gggaagtgct cacgcaccag cataatcagc cctggatcgg
    26881 acataatcag cgcatccggc cccatttcca ccaccggttt caggtcacgg ataaaggttt
    26941 tcagcttggc gttgtgcggt gcaatgttga ccacgacata aaactttttc cccagcgcgt
    27001 gggcttcatt gatgccgagc tgaagatttt cgtggttgaa ttcgttgttg cgcacacgca
    27061 gggagtaacg cggctggccc gcataaacag catctgcgcc ataagcgaaa gcgtaacgca
    27121 tatttttcag cgttcccgcc ggggaaagga gttccggttt aaacataatt ctctcgttct
    27181 gatgacaggt cagatccgct tcacctgatg aagcggccag gggagtgccc ccactttaag
    27241 ggcgggcatt gtagcgctgt aggggggagc tggtaaagcg ccggacgcca acaacacgcg
    27301 cccggcatcg taattacgta ttgtctttca ccgtctggct tgtgccattg gctttaacgg
    27361 aggcaattcc ggtttcacga gattgcgcgg tggcgtacat ctggctggag ccgataattt
    27421 gatgattggc ggctttcaga ttgaaataga atttgccgtt gcttgccgtt tttttctcat
    27481 agcgttcttc ttgcgggctg ttgctacgca ccgacgcgat gcccttttcc gcagaggctt
    27541 ttgaggtata cagctcgctg gtgaggatag tctcaccatt gcccgctttt agcacaaacc
    27601 gaaattgatt atcactgctc ttactgagtt caaaccaacc agccataatg actccttgat
    27661 tgaaattgcg tatcgaaaac agcatctaaa ggtgctgttt accgattaaa gtatgagcga
    27721 ttttctggtg cctgccaggg cggagagata atgtcagttt gctgaccata cgtgggataa
    27781 tgttgccgga tctgatctga acgccatctc cggctaacaa agtcatgtcg ctaaaactaa
    27841 acgatgcggc aagcgtcggc ttcccagcga taaccgacac cgtagacggc gcggataaaa
    27901 gactgctctt cgtccagagc ttccagcttg cgacgcaggt ttttaatgtg gctgtcgatg
    27961 gtgcggtcag ttactacgcg gtagtcgtca taaagatgat tgagcaattg ctcgcgggag
    28021 aacacttttc ctggttcatg agagagcgtt ttcaacagac gaaattccgc aggcgtcagg
    28081 tcaagcattt taccgcgcca tgaagcctga aaacgacctt cgtcgatcat caacgggctt
    28141 tcggcatcct gttgctgaag ttcgcgttgc ggtttacaac ggcgcaaaat ggttttgaca
    28201 cgcgctacca cttcccgtgg gctgtacggc ttgcagatgt agtcatcggc gccaatctcc
    28261 agccccagca ggcggtcgat ctcttcgatt tttgccgtca ccatcacgat cggaatgtca
    28321 gaaaaacgac gaatttcccg gcacagcgtc aggccatcgg tgccagggag catcagatct
    28381 aacaggatca gatccggtgg tgtctggcgc acatacggca gtacctgatc gccgtggctg
    28441 ataagcgtcg gcgcatagct cgcggcacgc agataatcaa tgagcaactg tcccagcttc
    28501 ggttcatctt ccacgatcaa aatacgcggt gtgttttcgt cgattggtaa ctcggtcata
    28561 cttctctctg taaatcccgt tccagcggta actctactgt aatgcttacc ccgccaaaag
    28621 gcgaatgggc agcaataatg cggccattat gtgcttcaac aatgttcagg caaatcgcga
    28681 gtcccagccc ggaaccgccg ctggcacggt tgcgggaacc ttcggtgcga taaaaacgtt
    28741 caaacaattt ttgtagctga tcgtcactga cgcccggcgc gctgtcggca aaggtcaggc
    28801 gcaccgtttt gtcgtgctgc tcggcagaga ttttcaggct gccgccgctg tcagtgtagc
    28861 gcaggctgtt ttccagtaag ttattgaaca actgcattaa acgatcgcga tcgccaaata
    28921 cggtaatact gtctggcagg gaaaattgca gtttcaggcc acggctggca aatcgttcgc
    28981 gaaatgcgcc gcccgccact tccagcagtg ggatcaaatc taccggtgct ttttgatagg
    29041 cgagagcgcc ttcatcagac atcgacaact gatgaagatc gtcaaccagt ttggtcagtg
    29101 taccgacctc cgcctgtaaa gaagccagcg tctccggcgt gaatttacgc acaccatcct
    29161 gaatggcttc cagttcgccg cgcagtactg ctaaaggcgt gcgcagttca tgagaaatat
    29221 cggccataaa atcgcggcgc atctgctggt ttttctccag tgtgctggcg agttgattga
    29281 aatcctctgc cagtctgccc aactcatctt cgctggtggg ggctacgcga gtagtgaaat
    29341 cacccgctgc cagtttgtgc gtgccatcca ccagtcgttt taccggagcc agcaaaccgc
    29401 gcgccagtgg gaatgtcgcc agcgccgcta ataaggtaga taaagcaaca atcagccaac
    29461 tggtttgccg ctgttgtctg tcaaaattga tatcagtatt acgggtcagt cgttcaacag
    29521 gggaggcgat caccgcgcca acttccgcac cattgaccat aatgggccgc cgtgtgccgt
    29581 cgggtggaac cggtgctcgc gggccaacca gcactttatt attttgatca accacccaaa
    29641 attgcgtgcg ccagccgtgt ggtggcatac cggggccggg cttatcttcg ttgttatcat
    29701 gctccagtga acgtaggatc tgaaatacaa agcgatcgtt attacgcagg aagcgccagt
    29761 tcccgtgctg ggcgtactgc tcaccaagcg catcgccgag catttgcagc cgttgttcat
    29821 taccatgctt gatgtaatcg ataaagccgc gctcaaaact gatacgtacc gcccagtgca
    29881 tcgtgatcaa taagacaatg caggtggcga aaatcgccag aaacagtttg ccggtaatac
    29941 cgggtcgcca gaacttcatt gcgcgctcct ttttcgccgc gaaatagcta cattttgatg
    30001 cgtatcgctc ggcactctgg caaagacaaa cgccggaagg gcgatgataa acgccatgct
    30061 aagccaggtg tacataaaga cggtttgtgt ggtgccgctg tcgacgctga catgctgtga
    30121 accaaaaagc cccagcaaca gcccggcgat agtgacgccg atactcatcg acaattgcat
    30181 aatcatcgac agcagactgt tgccgctgct cgccagattg tccgggagat ctttcagcgt
    30241 cagggtgttc atggaggaga aacgggtcga gttgaccatc ccttgtaaaa acaggacgaa
    30301 cggcaaaacg tagtaccagc ccagcagagc agtagtcata aacaacaggg tgaccagcga
    30361 cagacccagc gtggtcgcca ccagtacccg acgataacca aagcgattca ctacctgtac
    30421 cacaattcgc ttcatcccca tgctgccgag caccatcggg atcatcatca gtccggcatg
    30481 aaacggtgag aagccaaggc caatttgtag gaaaaccggc gtcataaagg gcaacatgcc
    30541 gctaccgata cgtccggcaa aactccccgc cagacccagc gaaaaggtac gagtacggaa
    30601 cagtttcaga ctgaacaggg cgcagttgtt atttctggcg tgcagcagat aaagcaccag
    30661 tgccaccacg ccaactgcga ccaggccagc aatcgccagc ggcgataaac ctgtaccttt
    30721 actgccgtcc agcgccaggg ttaataccgc catgccaacc gccagcaata aaaagccgga
    30781 gagatcaaag caccgcgtct gcatggtgta gttcggcatt aacatcagtg tggtgatcgc
    30841 gccaataatc cccaccggaa tgttgatcaa aaagatccag tgccacgatg cgtactccac
    30901 cagcagaccg ccgagcgccg ggccaagcag cggcccgatc tgaccgggca gcgtgacaaa
    30961 ggtcatcgcc gccatatatt gctcgcgcgg tacgattttc atcaccgtca acctgccgac
    31021 cggcaccatc atcgcgccgc caacgccctg taacgcgcgt gccagcaaca gttcgttcag
    31081 cgtgccggaa agcgcgcaaa acagtgaacc gagagtaaac agcacgatgg cggtaaagaa
    31141 aatattgcgc acgccgactt tgtccgccag ccagccgctg gcgggcagca tcaccgccac
    31201 ggtcagtaca taagagacaa tgaccatgtg catatgcaac ggactttccc cgaggctttg
    31261 cgccattgag ggaagcgcgg tgttaacgat ggtggtgtcc agcgactgca taaagaagcc
    31321 gaaagccaca atccacaatt gccagcgggt gctgtcggga agatctgtca tttactcggt
    31381 taccgcttgt ttaggtttac gcgaaaaacg cagtcgcaga cggtcgaaaa agagatacac
    31441 caccggcgtg gtatacagcg tcagaagctg gctcatgacc agaccgccga caatggtgat
    31501 ccccaggggt tgccgcagct ccgaaccgtc accgcccgac aataccagcg gcagcgcgcc
    31561 aaacagcgcc gccagggtag tcatcataat cgggcgaaaa cgcagcagac aggcctggaa
    31621 aatagcttcc tgcggcgtga ggttaccgtg ccgttgggct tcaagcgcaa aatcgaccat
    31681 cataatggcg tttttcttca cgatgccgat taatagcatg atccctatca gggcgattag
    31741 gctgaacggg gcattgaaca gctccagcgc cagcagcgct ccaacgcccg ccgagggcag
    31801 ggtggagaga atcgtcagcg gatgtacgta actctcgtaa aggataccca gcacgatata
    31861 caccgtggcg atggcggcga taatcaggat cacctgcgag ttcatcgtct cctggaacac
    31921 ctgcgccgtg ccggcaaaac taccgcgcac cgtcgaaggc acaccaagct gggtcattgc
    31981 gcgatcgatc gccgcactgg cgtccgagag cgatttcccg gtcggcaggt tgaacgagat
    32041 ggtcgaggct gccgataatc cctgatgatt caccgacagc ggagcattcg ccggttgcca
    32101 tttagcgaaa tacgacagcg ggatcgcttt tccttcgtta ttgataacga acattttttc
    32161 cagcgcactg atgtcctggg tatagcgcgg atccacttcc atcaccactt tatactggtt
    32221 catcggctgg taaatggtcg agatttgtcg ctgaccgaag gcgttattta acaggctgtt
    32281 ggcggcctgc acgtcgattc ccagccgtgc catggtgtcg cggtcgtaaa ccagattcat
    32341 ctccgcgccg ttatcctgct ggtcggagtt cacgtccgcc agttccggca atgtcgccag
    32401 ttttttgcga attttcggct cccattcgcg cagtgccgcc aggtcgtcgg acagcaacgt
    32461 gtactgatag ctggcgttcg actgacgtcc gccaacgcga atatcctgta ccgccatcag
    32521 gaacagattc gcccccggtt ctttcgccag cttcacgcgc agacggtcga taatttgctg
    32581 cgccgtttcg ctgcgttcgc cgcgtggctt cagcgtgata aacatcatcc cgctgttcac
    32641 ccgcgaaccg ccggtaaatc cggtgacatt atccactgcc ggatcgtcac ggataatttt
    32701 cataaaatcc tgcaacttac cgcgcatcgc ctgaaacgaa atactctgat ccgcctgtat
    32761 cccgcccatc aacacgccgg tgtcctgctc cgggaagaag gttttcggga tcgagatata
    32821 cagccagata ttcaaagcaa tggtgccaag cagcaccacg cccaccagac gggtatgatt
    32881 aagcacccat ttcagcgact taccatagcc ttgttgcagg gctaccaaca tgcgaccaaa
    32941 accacgtagt cgcttttgct cgcgcggctt actggctttt agcatccagc cgcacatcat
    33001 tggcgttagc gtcagagaaa ccagcagcga aataccaatg gcgacagaaa gcgtcacggc
    33061 aaactcgcgt aacaggcgac ccggcaatcc gcccatcaac agcagcggca gaaacaccgc
    33121 caccagtgac agactcatcg acagcaccgt aaaaccgact tcgcgagtgc cttgcagcgc
    33181 ggcctgtagc ggtttcatcc ccgcttccag gtgacgggca atgttttcca gcaccacgat
    33241 ggcgtcatcc accacgaaac cagtagcgat ggtgagcgcc attaacgaaa ggttattgag
    33301 actgaatccg cacaggtaca tcgccgcaaa cgtaccaatc agcgaaaccg gcaccgcaac
    33361 ggcgggaata atagtggcgc gacccgagcg caggaataaa aagaccacca gaatcaccag
    33421 cgccaccgag ataatcagcg tttgctcgac ttcctccagc gaggcgcgaa tggtggggga
    33481 gcgatcctgg gcaatttgca gatcaatcgc cgccggaatg gtctcctgca actccggtaa
    33541 ttttgcccgg atgctgtcaa ccgtctggat aatattggct tccggcagct tgcggatcat
    33601 cagcaaaata gccggtttgg cgttggtcat cccggcgttg cgcacatcct gcaccgagtc
    33661 ggtcaccgtc gccacatcgc ccagacgaag cgcgccgccg ttgttgtaat gaataatcaa
    33721 cggctgatat tcggcggcgg ttttcagttc atcgttagtc tggatctgcc agcgatgggt
    33781 atcatcttcc agcgccccct gcggcttacg cacgttggca ttgctgatgg cgctgcgcac
    33841 atcgtccagc gacacgccct gattaaacag cgcctgcgga ttcagcccga tgcgtacggc
    33901 gggcagtgag ctgccgccaa catcgacatc accgacaccg tcgatttgcg aaatcgtcgg
    33961 agccagctgc gtcgaggcga aatcgtacag ttcaccctgc gaataagtat cggatgtcag
    34021 cgtgaggatc ataattggcg catccgacgg gttcgctttg cgataggtcg gacggcttgg
    34081 catcccgctg ggcagcaaac tttgcgcggc gttgatcgcc gcttgcacat cgcgcgctgc
    34141 gccgttgata tcgcggtcaa aatcaaactg caaaataatg cgcgtgctgc cgagcgaact
    34201 gctggaggtc atttcactga ccccggcaat gcgtccaagc gaacgttcca gcggcgtggc
    34261 aacggaagac gccattgttt ctggtgacgc accgggcagc gaggcgctga ccataatcac
    34321 cggaaaatcg acctgcggca gcggggcgac tggcagcata cggaaaccca gtatgccgca
    34381 cagggtaatg gcgaccgaca gtaaaatcgt cgccaccggg cggtaaatga agagggcaaa
    34441 aaacttcact tacgcctcct cttcatgacg agcaaagcgg cttttggtcc acaatgccag
    34501 gcggtcgaat agcagataaa tcaccggcgt ggtgaacagc gtcagcacct ggctgacaat
    34561 cagaccgccg accatgccga tacctaacgg acgacgcagt tccgcgccga ccccggtact
    34621 caacatcagc ggcagcgcgc caagcagagc cgccagggtg gtcatcagga tcggacgaaa
    34681 acgcaacaga caagcctggt agattgcatc gcgcggcgac atgccttgct cgcgctcagc
    34741 agccagcgcg aagtcgatca tcatgatggc gttcttcttc acgataccga tcagcaaaat
    34801 aatgccgata atcgcaatca catccagctc gctaccggca atcagtaacg ccagcagtgc
    34861 gccgacccct gcggtgggta gcgtcgagag aatggtgatc gggtgaataa agctctcgta
    34921 cagaatgccg agcacgatat acatcgccac caccgccgcg acaatcagcc agacagtgct
    34981 gcccaacgcc gactggaagg cgagggtgct gccctggaac tgcgtggtga tatccaccgg
    35041 cagattcagc gtcttttcgg tgtccataat cgcctgcacc gcatcgccca gcgaatagtt
    35101 atccggcacg ttaaaggaga tggtcgttac cgggaactga tccagatggt tgatggaaag
    35161 cggcgcaaaa cgctgctcaa ttttggcaat tgagcttagc ggtaccacgc cgccgtcgct
    35221 gctggtcagg cgaatagtat ccagcgccgc aagcccagga gtattttcgg tgttgtgctc
    35281 cagcaccacg cgatactggt tggcctgagt ataaatagtg gaaatcagtc gctgaccaaa
    35341 cgcgttgtaa agggcgttat cgacgtccgc catgctgatc cccagacggc tggcgctgtc
    35401 gcgatcaaca ttgacatacg ccaccagccc tttgtcctgc cagtcgctgg agacatcaga
    35461 aagctgtggc agttgctgga gtttttccat caactgtggc acccaggtac tgagcgcatc
    35521 cagtgacgtg gcctgcaagg taaactggta ctgggtgcgg ctgacctgag tatcgatggt
    35581 caggtcctgc gttggttgca ggaagagatc gacgcccggc actttatcca ccgccgtttg
    35641 caggcgggcg atgacttttt gcacccgatc atcacgttca tccaacggtt tgaggttgat
    35701 ctgcaaacgc gcactgttca gcgacgggtt agtgccatca acgccaacaa atgaggtcag
    35761 actttgcacc gccggatctt gcaaaatcac atccgcgacc tggcgttgcc gctgggccat
    35821 attggtaaag gagctggatt gcggtgcctg caaagtgccc tgaataatgc cgttgtcctg
    35881 taccgggaag aaacctttcg gaatgaacac ccacaacagc acgctaagca gcagcgtgct
    35941 gagcgccacg cttaaggtca gccacggatg attcagcact ttcgcaagtc cctgaccata
    36001 ggcggcgatt atcctgtcga acattttttc cgaggcacgg gagaagcggt tctgtttacg
    36061 cagcgactcc tggctgagca tccgcgcaca catcatcggt gtcagggtca gcgacaccac
    36121 ggctgagatc aaaatcgcga ctgccagagt aatagcaaat tcgcggaaca gtcgcccgac
    36181 gatatcgccc ataaacagta gcgggatcag cactgcaatc agcgagaagg tcagcgagat
    36241 aatggtaaaa ccgatttcac ctgcgccttt gagcgccgcc gccagcggtt tttcgccttt
    36301 ttcgatatag cgggaaatgt tttcgatcac cacgatggcg tcatcgacca caaatccggt
    36361 ggcgatagtt aacgccatca gcgtcaggtt attgattgaa aaatcgagaa acaccatgac
    36421 cgcgaaggtg ccgattaacg acagcggcac tgcgacgccg ggaatgatgg tcgccggaat
    36481 attacgcaaa aacaggtaga taatcatgac taccagcgcg atagccatca tcaattcaaa
    36541 ctgagtatca tccacggatg cgcgaatatt ggtggtacgg tcagaaagta ccgtcacctt
    36601 caccgatttc ggcagactct cggtgagctg tggcagcatc tgccgaatgc tgtcggcggt
    36661 ggagataatg ttagcaccgg gctggcgctg aacattcatc acaatggcct gttctttgtt
    36721 agcccatgcg ccaagccagc tgttttctgc accttgctct acggttgcga catcgcccag
    36781 acgaatcggc gcgccgttct ggtaggcgat gattagctgg cgatactctt cggcggattg
    36841 catctggtcg ttagcggaaa gcgtaaccgc acgagaaggg ccgtcgaggc taccttttgc
    36901 cgagttgacg ttagcgccgg taatggcggt gcgcacggtt tcgctggtca ggccgagggc
    36961 ggcaatcgcc tgagcgttaa gtttgacgcg aacagccgga cgctgaccgc cggaaagtgt
    37021 caccaggccg acgccggaaa tctgcgagat tttctgcgcg acgcgggttt ccaccatatc
    37081 ttccacttgc gtcatcggca tggcggttga ggtgacagcg agcgtcatga tcggcggatc
    37141 tgccgggttc actttgctgt aaaccggcgg gttaggcaga tcgctcggca acaagttggt
    37201 cgcagcgtta atcgcggcct gcacttcctg ctcggcgaca tcgagcggta atgttagctg
    37261 gaactgcaaa gtgataactg acgcaccgcc ggaactttgc gacgacatct gtttcaggcc
    37321 agacatctgc ccaaactggc gttcaagcgg cgcggtaacg gcagaggtca tgacatccgg
    37381 gctcgcacct gggtagagcg tgactacctg aatggtcgga tagtccactt ccggcagcgc
    37441 cgaaacgggc agggctcgat aaccgataat cccggcgagt aagatcgcca ccatcagcag
    37501 cgtggtggcg acaggacgca taataaacag gcgcgacggg ccgcctgtgc tgctcggggg
    37561 taacacctgc atcaggagcg tgctcctttt ttcgcgtatt cgcggctggt ggctttctct
    37621 tccggagtgg tggcgctctg ggcttccact acttccactt tcgccccttc ggtcaggcga
    37681 tcaatgccgt ctgtcactac gcggtcgccc gcagaaatac ctgcgcggat caccactttc
    37741 tgactgtcct gaatgcccgg cgtcaccaga tgtttgctga ccttgttttc gctattcagc
    37801 acccagacaa aatggccttc attgcccatt tgcagggcgg ctgttgggat caccacagcg
    37861 ttttgctctg tgtcgactaa catgcgcgcg ttaacaaact gattgggaaa cagcgcatca
    37921 tcctgattat tgaagcgtgc tttcacttta atcgtaccgg tagtggcatc gatttggtta
    37981 tcgagactta acagcgtgcc ttcacttaat ttctttgaat tggtgcgatc ccaggcttca
    38041 acaaccagcg gttttccggc tttttgcgcc tgcactacgg tagcgatatc gctttccggc
    38101 agggtaaaga gcaaatcgat aggatgcgtc tgggtgatca ccacaatccc ggtggtatca
    38161 ccactggaga tttggttacc aacatcaacc tgcttgagac caacgcgacc atcgactggt
    38221 gcggtgatgc ggctccagtc gagttgcagc tgcgcgctgg cgacgcttgc ttcatcagcc
    38281 ttaatggtgc cttcggtttc actgaccagc gcctgttggg catccagctc ctgacgagat
    38341 acgagattgg tttttaccag ttgttgataa cgcgccaggt cacggcgggc gttggcaagc
    38401 gtggctttat cttttgccag ttggccctgg gcttgtgcta atgcaacttt gaactggctg
    38461 gggtcaattt ctgccagtaa atcgcctgct ttgacctgct ggccttcctg gaaatgcaac
    38521 gccatcagtt gaccgtccac gcggctgcgc accgtaacag tattagcggc ggtaatggtg
    38581 ccaagcccgg tgaggtaacg cggaactgcc tgttctacgg cggtcgccgc ctgaaccggg
    38641 gctaatgggc cggaacgcat accacggcga ccacccgctg gcgattgctg cgcttgtttc
    38701 gtcgcccctg gcgctgcact ccgggagtca ttgcggcctt gccagaacca gaatgcggcg
    38761 atggcggcga taaccaccac gattaccatt acccaacggg atttataact gcttttcatc
    38821 gttaagagtt tctcttcctg aaacgattcg cggaatgata ctagtgtagc cagccaatca
    38881 gggagaaaaa tggaggaatt atgaaacact atcgggaatc gtctgaatgg cgggcacatt
    38941 tgcgagcacc catccagtaa taacacagga aactatttta tctacgcgtt agcgatagac
    39001 tgcattcagg gcaaaaggag gtaggccgac gatttcagcg ggacgctgaa acgggcaagc
    39061 ccctcccgag gaaggggcct taaataagga aagggtcatg atgaagatac tcatcatcgt
    39121 ggtgctctta gtcgttagct acccagctta ctaagactac cagggcgggg gaaaccccgc
    39181 tctaccctca ctcctgaaag tatgccttca cggtccgatt gtcaatccgc aggctttgca
    39241 gtctgcgatc ctgccagcaa atattctttg cgagacgtca cgcaataatc acatgtgaaa
    39301 ctattttatc cgcaagtcag cgatagactg cattcagggc gaaaggaggt aagccgatga
    39361 tttcagcggg acgctgaaac gggaaagccc ctcccgagga aggggcctta aataaggaaa
    39421 gggtcatgat gaagtggatc atcatcgtgg tgctcttagt cataagcttc ccggcttact
    39481 aagactacca gggcggggga aaccccgctc taccctcact cctgaaagta tgccttcaca
    39541 attagattgt caatccgcag gctttgtagt ctgcgatcct gccagcaaat attctttgcg
    39601 agacgttacg caataatcac atgtgaaact attttatctg caagttagcg atagactgct
    39661 tgcatggcga aaggaggtag gccgccgatt tcagcgggac gctgaaacgg gaaagcccct
    39721 cccgaggaag gggccttaaa taaggaaagg gtcatgatga agctactcat catcgtggtg
    39781 ctcttagtta ttagctggcc agcttactaa gactaccagg gcgggggaaa ccccgctcta
    39841 ccctcactcc tgaaattatg ccttcacgat aagattgtca atccgcaggc tttgtagtct
    39901 gcgatcctgc cagcaaatat tctttgcgag tcgttacgca ataatcacag aggaaactat
    39961 tttgttcacg cgttagcgat agactgcatt cagggcgaaa ggaggtaagc cgacgatttc
    40021 agcgggacgc tgaaacggga aaccctctcc cggagaagag ggcttttaat aaggaaaggg
    40081 ttatgatgaa gcacgtcatc atactggtga tactcttagt gattagcttc caggcttact
    40141 aagaacacca gggggagggg gaaacctctc cctaaccctc actcctgaaa ttatgtcact
    40201 ccgcccttag tgtcaatcga cggctctccg ttcttgcgag gcgaatagca aaattttctg
    40261 cgccgccggg cctaattctg aaagtttctt cgtttgctct tatttcattg ttgcagtcgg
    40321 tatgataccg ggcctcaata attctctaag aaaggatttc ttatgagcga acaaatcacg
    40381 tttgccacca gcgattttgc cagtaacccg gaaccacgtt gcccctgcat tttgctgttg
    40441 gatgtttctg gttctatgaa cggtagacct attaatgaac ttaacgccgg attggttacc
    40501 tttcgcgatg agctgcttgc cgatccgctg gccttaaaaa gagtggaact cggcattgtg
    40561 acgttcggcc cggtgcatgt ggaacaacca tttaccagcg ccgctaattt tttcccgccc
    40621 atcctgtttg cccagggcga tacaccaatg ggggccgcca ttaccaaagc cctggatatg
    40681 gttgaggagc gcaaacgtga gtatcgcgcc aatggtattt cctattatcg tccgtggatt
    40741 ttcctgatta ccgatggcgc accaaccgat gagtggcagg ctgctgccaa caaggtgttt
    40801 cagggggaag aagataagaa gtttgccttc ttttccatcg gtgttcaggg ggcggatatg
    40861 aagacgctgg cccaaatcag cgttcgtcag cctttgcctc ttcaaggatt acaattccgc
    40921 gaactattta gctggttatc cagttcgctg cgttcggttt cccgctccac gccgggaacg
    40981 gaagttgtgc tggaagcgcc aaaaggctgg acgtcagtgt gagctggcgt ctggtctatg
    41041 catcaaccgt tggaacgtcg cacatcagcg cggacttgcc ttgccaggat gcctgccaga
    41101 tgcaggttgc ctggttaaat gaccagcagc cattattgtc ggtatttgtc gctgatggcg
    41161 caggtagcgc ctcgcaggga ggcgaaggtg cgatgctcgc cgtcaatgaa gcgatggctt
    41221 atatgtcgca aaaagtgcag ggcggggaat tggggcttaa tgatgttctc gcaacggaca
    41281 ttgtgctgac cattcgtcag cgactttttg ccgaggcgga agccaaagaa ttagcggttc
    41341 gcgattttgc ctgcacgttt ttgggattga tttcatcagc taatggcacc ttgattatgc
    41401 agattggcga tggcggcgtg gtggtagatt tggggcacgg tttgcaactg ccgctcacgc
    41461 cgatggtggg tgaatatacc aatatgacgc acttcattac cgatgaagat gccgtttcca
    41521 gactggaaac tttcaccagc actgagcgtg cgcacaaagt tgcagcattt acggatggta
    41581 tccagcggct ggcgttaaat atgctggata attctccgca tgtgcctttc tttaccccgt
    41641 ttttcaatgg gctggcgtca gcaacgcagg aacaactcga tttattacct gaattgttaa
    41701 agcagttttt gtccggcccg gcggtaaacg agcgtaccga tgatgataaa acgttggcac
    41761 tggcgctgtg gttaccatga aacccacttt atatactgct actggtgagt gcgttacgcc
    41821 aggctgtgaa ctgggcaaag gcggtgaagg cgcggtttat gatatcgagg agtttgtcga
    41881 tagcgtcgcc aagatttatc acacgccgcc acccgcctta aaacaggaca aacttgcctt
    41941 tatggctgcg acagctgacg cgcagttgtt gaattatgtc gcctggccgc aggcaacgct
    42001 tcacggtgga cgaggcggaa aagtaatcgg ttttatgatg ccaaaagttt ctggtaaaga
    42061 accgattcat atgatctata gcccggcaca tcgtcgtcaa cgttaccctc attgtgcgtg
    42121 ggattttcta ctctatgttg cgcgcaatat tgcttcatct tttgctacga ttcacgagca
    42181 cgggcacgtc gtgggtgacg taaaccagaa cagctttatg gtaggtcgcg acagcaaagt
    42241 ggtgttgatt gatagcgact cctttcagat taacgccaat ggcacgctgc atttatgcga
    42301 agtcggcgtg tcgcatttta cgccgccaga gcaacaaacc ttgccatcat ttgtcggctt
    42361 tgaacgcacc gcgaatcacg ataattttgg ccttgcgttg ctgatttttc acgtcttgtt
    42421 tggtgggcgg catccttatt ccggtgtacc gcttgtctct gatgcgggta atgcactgga
    42481 gacggatatt gcccatttcc gttatgccta cgcgtcagat aatcagcgac gtggtttaaa
    42541 accgccgcca cgatctattc cgctgtcgat gttaccgggc atgttgaagc catgtttcag
    42601 caggcattca cggaaagtgg cgtggcaacc gggcgtccga cggcaaaagc gtgggtagcg
    42661 gcacttgatt tactacgcca acagttaaag aaatgtaccg tttcggcaat gcatgtttat
    42721 cccggtcatt tggctgactg cccgtggtgt gctctggata atcaaggcgt tatctatttt
    42781 attgatctcg gcgaagaggt cattaccacc agcggtgatt ttgtgctggc gaaagtctgg
    42841 gcgatggtga tggcgtcagt agcaccgcca gcattgcaat taccattacc cgatcatttc
    42901 caaccgactg gcaggccgct tcctttaggc ctgttaaggc gtgaatacat cattctgatt
    42961 gagatcgcac tgtcagcgtt atcgctgctg ctttgcggcc ttcagacaga accacgttat
    43021 attattttgg ttcctgtgct ggcggctatc tggattattg gcagcctgac aagcaaagcg
    43081 tacaaagcag aaatccagca acgccgtgag gcgtttaatc gcgcgaaaat ggactatgac
    43141 catttagtca gccagatcca gcagttgggc gggctggaag gttttatcgc caaacggacg
    43201 atgctcgaaa aaatgaagga cgaaattctt gggttaccgg aagaagaaaa gcgcgatctg
    43261 gcagcacttc aggacaccgc aagggaacgg cagaagcaga agtttctgga gggatttttt
    43321 attgatgttg cctctattcc cggcgttggc cctgcgcgta aagcggcgtt acggtccttt
    43381 ggtattgaaa cagcagcgga tgttacccga cggagcgtta agcaagtgaa aggttttggt
    43441 gatcatctga cccaggcggt tatcgactgg aaagcgagct gtgaacgccg ttttgtgttc
    43501 aggccgaacg aagcggtaac gcctgcagac agacaagcgg taatggcaaa aatggccgcc
    43561 aaacgacatc ggctggaatc ggcgctgaat gtcggtgcga cagagttgca gcgattccgc
    43621 cttcatgctc cagcacggac catgccgttg atggaaccgc tacgtcaggc ggcagaaaaa
    43681 ctggctcagg cgcaggctga tttaagccgt tgctgagcca gttttcaatt aacgaaacac
    43741 cacttccgcc cagcgtgcca gcccggcggt gacggagcca aagtcatcgc cacctgcaat
    43801 cggaatgccc ggcaactgtt ctgtcagcgc ttttttaatc agcggtgaac gggcgctacc
    43861 gccggtcaga tagataacat ccggtttttc ctgggcgtta tccagcgcca gttgcacctg
    43921 ttccagaatc cgcgccagtg gctggctgag ggcgctttcc agcccttgct ggctaatcag
    43981 cgtagccagt tcatcgctga taaacggcag tgaggcgcgg gtttccgcta cgcttgaaag
    44041 cgcaattttg ctctcttctg cgctacgtac caggcgatag cttaaacgct gacgccagac
    44101 tttctgtaac agggccactt tttccggttc gcgggcatcg cgtaccaggt cgttaagcag
    44161 acgaccgttg gcactactgt agaaatcact ttgcgcagga acgtcgttga tggcaaccgc
    44221 attccaccac ggcagaatcg gcagggcgat gcctttttcg gtttcgccac ccatgcccag
    44281 taacggcatc aggtttttaa acgccagtgc gatatccaga tcgttaccgc caatacggca
    44341 accactgtga cccagcaggc tggcttcacg atcgagacgc gaacgccact gcggccccat
    44401 cagcagcaat gaacagtcag tcgtaccacc gccgatatcc accaccagca cccgtttttc
    44461 ttcctgcaag gtggcttcgt aatccagccc agccgcgacc ggctcgtact ggaataccac
    44521 atccttaaac ccggcacgct tcgccgcgcg ttccagaatc ccttgcgcct gggcgtttgc
    44581 ttcatcgccg cccagcccct ggaagttgat cgggcgacca atcaccgcct gagtaattgc
    44641 ctccggcagc tgcgcctgcg cctgctggcg aatgtgcaac atcattgcgc agaccagatc
    44701 ctcaaacagc gctacctgct gcggttttaa gccgctggca ccgaggaatg attttggtga
    44761 tttcacaaac cacacctctt ctggatcatc aatgtactgt gccagtgagg aaagaccgaa
    44821 ctgcacgctt ttcgccgtaa catcgatatc ttcttcgcgg ttataacgaa tcgcccgacg
    44881 caacagcgcc tgcgtttcat cgtcgtctgc cggaacatca tgatggcggt acagccattc
    44941 gcttaccgct tcacgcgttg gcgcgcaaag cattgaaggc agcagcgtgc tgtcgttttc
    45001 cattttcagt aattgcggct taccgtcgcg catgaccgcc actgaacagt ttgctgtacc
    45061 gtaatcaaaa ccaataaaca cgaaataatc cccatgccgg tgaagaaggg gcgtgacttt
    45121 agcgaaatgt tgccgtcgcg acaaccggaa tatgaaagca aagcgtagcg tctgaataac
    45181 gtttatgctg aaagcggatt aacaaggaga tgcgatgtat accctgaact ggcagccgcc
    45241 gtatgactgg tcgtggatgt tgggatttct cgccgcccgt gcggtgaacg gtgtggaaac
    45301 ggtcgctgac gattattatg cccgtagtct ggcggtgggc gaatgtcgcg gcgtggtgac
    45361 tgctattccg gatatagcca gccatactct gcacataaat ttaagtgcag gtttagaacc
    45421 cgttgccgca gagtgtctgg cgaaaatgag ccgcctgttt gatctgcaat gtaacccgca
    45481 gattgttaac ggtgcgttgg gcaagttagg cgcggcacgg cccggattgc gtttacccgg
    45541 ttgtgttgat gcttttgagc agggcgtgcg ggcgatttta ggccagctgg tgagcgtggc
    45601 gatggcggca aaattgaccg ccagagtggc acagctttat ggcgaaaggc tggatgattt
    45661 tccggattat gtctgcttcc ccacgcctca gcggctggca gcagcagatc cgcaggcatt
    45721 aaaagcgtta ggtatgccgt tgaaacgggc agaggcgctg attcatctgg caaatgcggc
    45781 gctggagggc accttaccaa tgacaatacc gggcgatgtg gagcaggcga tgaaaacgct
    45841 gcaaactttt ccgggtatcg ggcgctggac ggcgaattat ttcgccttgc gcggctggca
    45901 ggcgaaagat gttttcctgc cggatgatta tctgattaaa cagcgatttc cgggaatgac
    45961 accggcacaa atccgccgtt atgccgaacg ctggaagccc ttgcgttctt atgcgctgtt
    46021 gcatatctgg tatacggaag gctggcaacc agacgaagca tgacgttggt cgccggatgc
    46081 ggtgcgagcg ccttatccgg cctggggaat gagcgatttg taggcatgat aagacgcgtt
    46141 agcgtcgtat caggcgatgg agaagcacgc ctcagttaat cgcgaaataa ctgctattca
    46201 ccagcaaatc cagcggttgg gcatcggcaa tcacatcgcc ataaatcaga tcgacgccga
    46261 tgccagaaag cgtatccatc accaggggta aaactactgg cccggcgatg gtcttcatcc
    46321 cgagccgctg agcgtgcccc tgaatgatcg taatcagcat ctcatccatc aaatttccct
    46381 gcacattggc gcataactca ccatcaagta gcaggtaatc tgccatattc gctttcagcg
    46441 agttgaagat ttgcagatcg cggcccacct gactgaatac gatccgacat cccgccagcc
    46501 gcagtttttg cacgctttct gcgtgatcta aaatcgcgtc tgccggaata atcagatgta
    46561 ataaccgtgg gggtagaggg ctattttcca gctgctcaag cagttcattc accagcgtgg
    46621 cgctactcaa accggcaacg gaaaggggga gggcgatgct taagccttta ctggcaatcg
    46681 cttttgcggc ctgctggaaa aaatcgtgga atacccgtcg gtcaagagca tggctaagtg
    46741 ccggatcgct gaagctacga cgaaatgttt gttcatcaat aatctcgcct tcgcaactcc
    46801 agagcttaag tgaaatcagc cacaaattac gcgcttccgg gatccgcggc gaagcgacac
    46861 cgtgggcgat catcatcaac tgattctctt taatcatccg ccactgttca tcaagcgaca
    46921 tcaccgcccg ttcgctatgt gcggcagctt gctgcggttc gtaaaccgtc acccggcccc
    46981 gtccaccatt tttggaggca taacaggcga tatcagcctg cgacatcact tcagccgcct
    47041 gatgattgtt gtcatcaatc aaggtaatcc cggcactggc acctacccga tgtacacgtc
    47101 cttcccagat aaagtgatag tcattcacgg cgctgataat gcgtgtagcg ataaaacgcg
    47161 cactttcgac attacaatct ggcagcagca gaccaaattc atcgccaccg agtcgcgcca
    47221 gcacgtcgct ggagcgcagc atactcagca tcaatgacgc cagttcgcgc agtaaagcgt
    47281 cgccagcggc gtgcccggcg ctgtcattca ccgctttaaa gcgatcaaga tcgataaaca
    47341 ccagtgcatg tcgctgatgt gtactgttta ccgtttgcag caggatgcgt agttgcttct
    47401 caaaactggc gcgattggcg agatgcgtca gtgcatcatg ggaggcgctg tagctcagct
    47461 ggcgcagcat tttacgcgat tcggtgacgt cctgaatcac cagaaccgag cctatattgc
    47521 tgccgtccag agtacttaac ggcgtaatac tgtaatgcac gtcgtagctg ccgccgctcc
    47581 gacagtgcaa caccacatct tgttcaatcg cggaacgtga ggtgtcggca ctgtaaatgt
    47641 tctccattaa tggtccgttg tcgccaaaag taatatgcaa caccgtcagg agcggaacac
    47701 ctaacgcttc ttcctgcgtc cagccgctca tcttctccgc caccggattc ataaaggtaa
    47761 ttttcattgc catatcaata cagaccacgg cttcgccgat ggaatccagc gtaatgtgca
    47821 ggcgctcttt ttcctgaaac aatgcctcgt taagctgttt cacttcggtc atatccatat
    47881 taatgccgag caggcgttcg acttcgcctt ctttattcag tacccggttg gcgagggcgc
    47941 ggatatggcg gatgccatct ttcacggcaa tgcgaaattc cagcttaaag ggcgagcgtg
    48001 attgcaacga atcacgaatc actttttcgg catgctggcg atcttccggc agtacgcact
    48061 cgtaccacac ctgccagttc ggtttgatat gcggaggaat ttcatacagc tcgaacatcc
    48121 gcttatccca gctaaaaata ttcggcttta gctcccactc ccagatgcca atcccgcccg
    48181 cttcgttagc cagcgtgatg cgctccatca gttgctggtt cacctgttcg gtgcgtttta
    48241 gctcgttaat gtcttcaatc tgagcgataa aatagagcgg cgtaccatcg gtgtggcgca
    48301 ccagtgagac ggcaagcaac gcccagacaa catcgccatt gcggttgtag tagcgttttt
    48361 ccattgaata ggtgtttatt tcaccgctga tcagcttttc aacctgttgc agatctttat
    48421 tgagatcctc cggccaggtc agttgctgaa aggtgagtcc gcgcagctct tcctgactgt
    48481 aaccgagaaa ctggcagagc gctttgttgg attgcagcca ttgtccctcg gtgcccacta
    48541 atgccatgcc gatggcggaa tattccatcg cgttccgaaa gcgggtttcg ctttcggaaa
    48601 tgtgtttgcg ttccgcacga aaggcataca tcaccatggt catgatgttg gcgggcagca
    48661 ggatcagcaa aaacggtagc cacggcatat ggctcatcag gtatgtacgc ggcgtagcaa
    48721 gcagggaggg atccgcggcc atcatcagtg acaccatcat caccgtggta aggaagatca
    48781 aaaaggcttc cattcgtggc aggcgcacgg cgctccacat caacagcaca ataatgaaag
    48841 taaaaggcca cggcagatac agcatcgaaa gccagcttaa cgtcagtgtg atggctaacg
    48901 tgagcagcga ctcaaaaagc aaccgtgggt tgcgatggcg cagcagatag tgtggtttaa
    48961 ataacaatcc cagcggcacc agtgccagtg cgccgatgga ttctgacagt acccatatca
    49021 aaaatgccct gagaggatcg tctccgggcg tcagcaggat aaccagaaca ccccctaaca
    49081 gaggtggaac aatggcgctg ccgagtgcca gacgcagcca gtcagccaga ttttgcaatg
    49141 ggttatacca cggcagcaat ttacgcagta gcactgcccc gaccacggct tcaacaatat
    49201 tgatggtcgt ccaggtcatg ttcagcgagc tggtggaaaa aagcaggatg gatgcggcga
    49261 tatttcccag cgagcagctc agcgcaatcc ccggccacat gcgcccggca tggcgataaa
    49321 acgccaccat catgatggac gtcgggaacc acagtggggc gagttgggtg ccaaactgcg
    49381 aaagctcaag cgagaaaagg gtaaagataa acgagactaa acctaaactg accaggtgaa
    49441 gcagcgggtg gggcagggca attaatacat gctgtgattg tttgctcatt accgctttat
    49501 ccgcaagacc aggtcgccag tattcgtttc tgtgtttatg ctagtacaaa agttttacat
    49561 tttatatgcg aattgctcat aaagtgacgt aaaggcggat aatttgcgca actgcgtata
    49621 acaattttta ccttgtataa aactgatcaa cgtaatttgc caccgggaat tcttcgtcaa
    49681 caattaactc tgagcgcgcg gcaacgctat tcgactggta tcaggcggat gaaatcccta
    49741 taattgccgc gtttggcgct tcgtcgcccc cttcctaaca tccaggttaa tcaggtcgct
    49801 aaatatatga ctgatcagtc tcatcagtgc gtcattatcg gtatcgctgg cgcatcggct
    49861 tccggcaaga gtcttattgc cagtaccctt tatcgtgaat tacgtgagca agtcggtgat
    49921 gaacacatcg gcgtaattcc cgaagactgc tattacaaag atcaaagcca tctgtcgatg
    49981 gaagaacgcg ttaagaccaa ctacgaccat cccagcgcta tggatcacag cctgttgctt
    50041 gagcatttac aagcgttgaa acgcggctcg gccattgacc tgccggttta cagctatgtt
    50101 gaacatacgc gtatgaaaga aacggtgacg gttgagccga agaaagtcat cattctcgaa
    50161 ggcattttgc tgctgacgga tgcgcgtttg cgtgatgaac ttaacttctc cattttcgtt
    50221 gataccccgc tggatatctg cctgatgcgc cgcatcaagc gtgacgttaa cgagcgtggg
    50281 cgttcaatgg attcagtgat ggcgcaatat caaaaaaccg tgcgtccgat gttcctgcaa
    50341 ttcattgagc cttctaaaca atatgcggac attatcgtgc cgcgcggcgg gaaaaaccgc
    50401 atcgccatcg atatattgaa agcgaaaata agtcagttct ttgaataagc ttgataaatt
    50461 gtgtaccgtt cagtgataac ctggtatgcc cttgacgtaa ttggcgttaa gggagtgatg
    50521 cgcgaaagga gaaaatgcca tgcgtctgtg tgaccgagat attgaagcct ggcttgatga
    50581 aggccgtttg tcgatcaacc cacgtccgcc agtggagcgt attaacggcg cgacggtgga
    50641 tgtgcgcctg ggcaataaat ttcgtacctt ccgtggtcac acggctgcgt ttatcgatct
    50701 gagcggtccc aaagatgaag tgagcgccgc gcttgaccgc gtgatgagcg atgagatcgt
    50761 actcgatgaa agcgaggcgt tttatcttca cccaggagag ctggcgctgg cggtgacgct
    50821 ggagtcggtg acgctgccag ccgatctggt gggatggctg gacggtcgtt cctcactggc
    50881 gcgtctgggg cttatggtgc acgtcaccgc gcaccgtatc gatccgggct ggtctggctg
    50941 tattgtgctg gagttctaca actccggtaa gctgccgctg gcgctgcgtc cgggcatgtt
    51001 aattggtgcg ctgagctttg agccgctttc cggcccggcg gcgcgacctt acaaccgccg
    51061 tgaagatgcg aaatatcgca accagcaggg cgcggtagcc agtcgaatcg ataaagacta
    51121 attcaggccc attgaggata ccatgagacg atttctgacg acgctgatga tactcctggt
    51181 cgtgctggtg gccgggttat ctgcgttagt gttgctggtg aatccgaatg atttccgcga
    51241 ctatatggtc aagcaagttg ctgcacgtag cggttatcaa ttgcagctcg acgggccact
    51301 gcgttggcac gtctggccgc agcttagtat cctctccggg cgaatgtctc tcactgccca
    51361 gggcgcgagc cagccactgg ttcgcgcaga caacatgcgt ctggacgtgg cgcttttacc
    51421 actgctgagt catcaactga gcgttaagca ggtgatgcta aaaggggcag tgatccaact
    51481 gacgccgcag acggaagcgg tgcgcagtga agacgctccg gttgcaccgc gcgacaatac
    51541 cttgccggat ctgtcagacg atcgcggatg gtcgtttgat atatccagtc ttaaggtggc
    51601 ggacagcgtg ctggtgttcc agcatgaaga tgacgagcag gtgacaatcc gcaatattcg
    51661 cctgcaaatg gaacaagatc cccaacatcg tggttcattt gagttctccg ggcgggttaa
    51721 tcgcgatcag cgcgatctca cgatatccct taacggtacg gtagatgctt ctgattatcc
    51781 gcatgattta acggcggcta ttgaacaagt taactggcag ttgcagggtg cagatttacc
    51841 aaaacaaggt attcaggggc aggggagttt ccaggcccag tggcaggagt cacataaacg
    51901 cctttcattt aaccaaatca gcttgaccgc caatgacagt acgctgagcg ggcaagcaca
    51961 ggtcacgctg acagagaaac cggaatggca gctgaggctg caattcccgc aactgaatct
    52021 tgacaacctc atcccgctta aagaaacagc gaacggtgaa aacggtgccg cgcagcaggg
    52081 gcagagccaa tccacgttgc cgcgcccggt catttcttcg cgtattgatg aaccggccta
    52141 tcagggactg caaggcttta cggctgatat tttgttgcag gccagtaacg tgcgctggcg
    52201 cggaatgaat tttacagatg ttgccacgca aatgaccaac aagtcgggtc tgctggaaat
    52261 tactcaactg cagggcaaac ttaacggtgg acaggtttca ctgccgggca cgctggacgc
    52321 gacatcaata aatccgcgaa taaacttcca gccacggctg gaaaacgttg agattggcac
    52381 cattctgaag gcgtttaact atccgatttc gttgaccgga aagatgtcac tggctggtga
    52441 tttctccggt gctgacatag atgccgacgc attccgccat aactggcaag gacaggcaca
    52501 tgtcgaaatg accgacacgc gcatggaagg gatgaacttc cagcagatga ttcagcaagc
    52561 ggtagaacgt aatggtggtg atgtgaaggc cgctgaaaac ttcgataacg taacgcgtct
    52621 tgaccgcttt accaccgatt tgacgttgaa ggatggcgtc gtgacgttaa acgacatgca
    52681 aggtcaatcg cctgtgctgg cgctgacagg ggaaggcatg ttgaatctgg cagatcaaac
    52741 ctgcgacacc cagtttgaca ttcgtgtgat tggcggttgg aacggggaaa gcaaactgat
    52801 tgatttcctc aaagaaacgc cagtaccgct gcgggtttat ggcaactggc agcaacttaa
    52861 ttacagcctg caagtggatc agttactgcg caaacatcta caggacgaag cgaaacgtcg
    52921 cctgaatgac tgggccgagc ggaataaaga ttcccgcaat ggcaaagatg tgaagaagtt
    52981 gctggagaag atgtaagaca agttatgcag atggcggcgt caatttgtcg ccatctgtat
    53041 ttatagtact aagcagcatg tggatattga acttcaatac gttgaatatg caatcctgca
    53101 tgagccatca tggtgatcaa tttatcgata ctaaagatct ggatcttacc tctggcaagt
    53161 tcagatatgc gaggttgagt gacccctaaa gcagacgctg cctgagcctg gctaaaccct
    53221 tgttgttcaa tccagttatt aatgatcgta acaagttcgg cacggatccg catattttcc
    53281 gcttgttcag gggtgtcact caccgcatcc cacacactat caaaggtttc aattttcatt
    53341 tttccgctcc tgaacaagtt ctttatagcg tcgtttggct aaatcaatat tcgattgact
    53401 ggtagtctgt gttttctttt gaaaacaatg caatacgtaa accgcttctt caaattttgc
    53461 gacatacatg acgcgataga tcccatcagc atcgcgagta cgaatctctc gcacaccagg
    53521 gccgatagtt gaaaaaggtt tccagtcgta tgggtttaac ccttgttgga tcctgtcgag
    53581 ttgatagcca atttcctgtc gagacgattc tggaaatgcg cgtaaatcgt caagactggt
    53641 atcaagaaaa gccagctttt ttctcattac tccatcctta gattatacta aattttgtat
    53701 aaatcaaatg cgcagtttga acctgcgcat ttgatgtttt atcacacctc gtactccatc
    53761 tcgccatcct tatgcagcgg aataatctgc accttctgca cgcgatggct ttccacctgc
    53821 aacgttttaa gcaaataatc ccccacctga acttcttcgc caggttttgg aatacgttgc
    53881 aaatattcca tcaacagccc ggcaatggtg tgatattcgc gtttctcatc cagcggtagc
    53941 ggcacatatt gcaccagatc ttccagcggc atatgaccat tcgccgtcca ggaaccgtcc
    54001 gcattcttct gaatatcatg gcgggcgtcg atctcttcca cttcgttcgg taagttaccg
    54061 gcaatggttt cagtgacgtc acttaatgtc acaatccctt ccaccgagcc aaactcatcc
    54121 accacaaaag caaagtgtgt gcgggcatta cggaactgct ccagggcagg taacaacggc
    54181 aaggtttccg ggaacaccag cggctggcga atcaacaccc gcaggttgag cggttcgccg
    54241 cgcagagact gttgtaacag gtcgataacg tgaacaacac cgagcaaatc ttctgcgtca
    54301 tcgccatcag taacgacaag ccgcgtatgc tgatttcgct ccagtaactg gcggatttct
    54361 tcttccggcg cgttgagatc gatatgctca atatcgtggc gcgacgtcat aatgctgctg
    54421 acggtacgct ggttaagatt aagtacccgc tcaatcatcc gccgttcctg cggattaaag
    54481 atctgctggt taccatgatc caccagcata gacgcggttt cggcgtccag ttccgcatct
    54541 tctttttgcc cgctaagcag gcgcattacc gcttcggtag tccgctggcg cagtgtctgg
    54601 ttagcggaaa gaaaacgtcg ccggttaaag atagccaact gattgagcgc ctcgatcatc
    54661 accgagaaac caatggcagc gtacaggtag cctttcggaa tgacgaagcc gaaaccttct
    54721 gccaccaggc taaagccaat catcaacagg aaacttaggc agagaataac gatggtcggg
    54781 tgactgttaa caaattgcgt taacggcttg ctggccatca acatcaggct gatagcgata
    54841 accacggcgg ccatcatgac cagtaaatgg tcgaccatcc cgacggcggt aatcaccgag
    54901 tcgagtgaga agatggcgtc cagtaccaca atttgtgtca ccacccccca gaacttcgca
    54961 cctttgcgtt gggtggggtt attgctgtct ttcccttcca gccgttcgtt cagctccatc
    55021 gtggctttga acaacaggaa gaaaccacca aacagcatga ttaagtcgcg ggcgctaaag
    55081 gtaaacgagc ggaagctgaa cagcggttga gtcagggtga ccagccagga gattgacgcc
    55141 agcagtaaca ggcgcattaa catcgccagc agcagcccgg taacccgtgc gcggtcgcgc
    55201 tgcttcggcg gtagtttttc ggcgaggatg gcaataaaga ccaggttatc aatgccgagg
    55261 accagttcga tcacaatcag cgtgattaac ccggcccaga tagacggatc ggcaatccat
    55321 tccatagtaa gtacaaaacc tattcgttgt atgacaaatg tcacaattcg atcatggata
    55381 aatcggggca aaaatgcaat gttgcagcac tatttatctc atattatttg aggtttataa
    55441 cctcacatta tccctgaatt aaaagtggta ataataaaac attatttaag aaatatcgca
    55501 tgtggagaca ctatattaaa tattgtcaac ctaaagaaac tcctaaaaac catattgaat
    55561 gacacttaat ataattctta aaaatagcca attgccgaat tgttaccttg cctgctattc
    55621 cgttagctgt aacacttcct cctgcattat tggagagcca atattcaatt accataatgt
    55681 caccttatat tcggaagtta atttccgcaa taaccctttc tcatcaacga ctgtatgttg
    55741 agagggtttt aaaaattaag ctaaatcagt gtattggtag ctaaaaagcc aggggcggta
    55801 gcgtgtctgg atgcctgaaa gaccagtctg aattattctg tcaatagcct gcggatgaaa
    55861 tcaatttttt taggactgat gccagttaaa tttttattca attaactgca aggtaaatgt
    55921 gcatggcgac attgctatta atagtgcaca ggataattac tctgccaaag tgataaataa
    55981 acaatgatga aatccaaaat gaaattgatg ccattattgg tgtcagtaac cttgataagc
    56041 ggttgcacag tacttccggg cagcaatatg tcgacgatgg gtaaagacgt catcaaacag
    56101 caggacgctg atttcgatct cgacaaaatg gtgaatgttt atccgttgac cccgcgcctg
    56161 attgatcaat tacgcccacg cccgaatgta gcgcgcccca atatgacgct ggaaagtgag
    56221 atcgcgaatt atcagtatcg cgtcgggccg ggggacgttc ttaatgtcac cgtctgggat
    56281 cacccggaac tcaccacgcc agccggtcag taccgcagct ccagcgacac cggcaactgg
    56341 gtacagcctg acggcaccat gttctacccg tatatcggca aggttcacgt cgtggggaaa
    56401 acgctcgctg aaatccgcag tgatattacc ggtcgcttag cgacgtacat cgctgacccg
    56461 caggtggacg ttaatatcgc cgccttccgc tcgcaaaaag cctatatctc aggtcaggtg
    56521 aataaatccg gtcaacaggc gattaccaac gtaccactga ccattctcga tgccatcaac
    56581 gccgcaggtg gcctgaccga caccgctgac tggcgcaacg tggtgctaac acacaatggt
    56641 cgtgaagaac gcatttcttt gcaggcgctg atgcaaaacg gcgacctcaa tcagaaccga
    56701 ctgctttacc ccggcgatat tctctacgtg ccgcgtaatg atgatctgaa agtgtttgtg
    56761 atgggtgaag ttaaaaaaca gagcaccctg aaaatggact ttagcggcat gaccctaact
    56821 gaagccctgg gtaatgctga aggcatcgac atgaccacct ccaacgccag cggcatcttt
    56881 gtcattcgtc cgttgaaagg cgagggcggg cgtaacggca agattgccaa tatctaccag
    56941 ctggatatgt ccgatgccac atcgctggtg atggcgacag aattccgcct gcaaccttat
    57001 gacgtggtgt atgtcaccac cgccccggtt tcccgctgga accgtctgat caatcagttg
    57061 ctgccaacta ttagcggtgt ccgttacatg acggatacag ccagcgacat tcataactgg
    57121 taatcgtcat gtttaacaac atcttagttg tctgtgtcgg caatatttgc cgttccccga
    57181 cggcggaacg cttactgcaa cgttatcacc cggagctgaa agtggagtcc gctggactcg
    57241 gcgcgctggt cggtaagggc gctgacccta ctgctatcag cgtcgccgca gaacatcaac
    57301 tgtctctgga aggtcactgt gcacggcaaa tcagccgtcg cctgtgtcgc aactacgacc
    57361 tgattttgac catggaaaag cgccatatcg aacgcttatg cgagatggca ccggagatgc
    57421 gcggcaaagt gatgctgttt ggtcactggg ataacgaatg tgaaatcccc gatccgtatc
    57481 gcaaaagccg ggaaacgttt gtagcggtgt acacattact tgaacggtct gcccgccagt
    57541 gggcgcaggc attgaacgca gagcaggtat aagaatgaca gaaaaagtaa aacaacatgc
    57601 cgctccggta acgggcagtg atgaaatcga tattggtcgc ctggtcggca ccgtcattga
    57661 agcgcgctgg tgggtgattg gcatcaccgc cgtatttgcc ctctgtgccg tggtttacac
    57721 cttcttcgct acgccgatat acagcgccga cgcactggta caaatcgaac aaagcagcgg
    57781 caattcgtta gtgcaggata tcggatcggc attagccaac aaaccgcctg catcggacgc
    57841 cgagatccag ttgattcgtt cgcgcctggt gcttggtaaa acggtggacg atctcgacct
    57901 cgatattgcg gtgagcaaaa acacgttccc gattttcggt gcgggctggg atcgcctgat
    57961 gggacgccag aacgagacgg taaaagtgac cacctttaac cgtcctaagg agatggcgga
    58021 tcaggtgttt acgcttaatg tgcttgatga caaaaactac actttgagca gcgatggcgg
    58081 ctttagcgcc cgtgggcaag cgggccagat gctgaaaaaa gaaggcgtca cgctgatggt
    58141 tgaagctatt cacgccagcc cgggcagtga gtttaccgtc accaaatact ccacgctggg
    58201 gatgatcaat caactgcaaa acagcctgac ggtaacggag aacggcaaag atgctggcgt
    58261 tttgagcctg acttatactg gagaagatcg cgaacagatc cgcgacattc ttaacagtat
    58321 cgcccgtaac tatcaggaac aaaatattga gcgcaaatcg gcggaagcgt cgaaaagcct
    58381 cgctttcctc gcgcaacagt taccggaagt acgtagccgc ctcgatgttg ccgaaaacaa
    58441 actgaatgcc ttccgtcagg ataaagattc agttgatctg ccgctggaag cgaaagcggt
    58501 gctcgattcg atggtgaaca tcgacgcgca gttgaacgaa ctgaccttta aagaagcgga
    58561 aatctccaag ctgtacacca aagttcaccc ggcgtatcgc acgctgctgg agaaacggca
    58621 ggcgctggaa gacgaaaaag ccaaacttaa tggtcgcgta acggcgatgc cgaaaaccca
    58681 gcaggaaatt gtccgtctga cccgcgatgt cgagtctggg cagcaggtct atatgcaact
    58741 gctgaataaa gagcaggagc tgaaaatcac cgaagccagc accgtcggcg atgtgcgcat
    58801 tgttgacccg gcaatcaccc agcctggtgt gctaaaaccg aagaaagggc tgattatcct
    58861 tggggcgatt atccttggcc tgatgctctc tatcgtgggt gtgctgctgc gctcgttgtt
    58921 taatcgcggt atcgaaagtc cgcaggtgct ggaagaacac ggtatcagcg tctatgccag
    58981 catcccgctg tcggagtggc agaaagcgcg cgatagcgtc aaaaccatca aagggattaa
    59041 acgctataaa cagagccagc tactggcggt ggggaatcca accgatctgg cgattgaagc
    59101 catccgtagt ctgcgcacca gtctgcactt cgcgatgatg caggcacaga acaatgtgtt
    59161 gatgatgacc ggggttagcc cgtcaatcgg taaaaccttt gtctgcgcca acctggcggc
    59221 ggtgatcagt cagaccaata aacgggtgtt gttgatcgac tgcgatatgc gcaaaggcta
    59281 cacccacgag ctgttgggca ctaataacgt caatggcctg tcggaaattc tgattggtca
    59341 gggcgagatt actaccgctg ctaaaccgac ctctattgcc aaatttgacc tgatcccgcg
    59401 cggtcaggta ccgccaaatc cttcggaact gttgatgagc gaacgttttg ccgaactggt
    59461 gaactgggcg agtaaaaact acgacctggt gttgattgat acgccgccga ttctggcagt
    59521 gaccgatgcg gcaattgttg gtcgtcatgt cggaaccacg ttgatggtgg cgcgttatgc
    59581 ggtcaacaca ttgaaagaag tggaaaccag tctgagccgc tttgagcaaa acggtattcc
    59641 ggtgaaaggg gtgattctga actccatctt ccgtcgcgcc agcgcgtatc aggattatgg
    59701 ctattacgaa tacgaatata agtcggacgc gaaataacga aatccgctgt cggatgcgat
    59761 gctgacgcat cttatccgac ctacgttcac gccgcatcag gcaagcaaac ccgctccgta
    59821 ggtcggataa ggcgttcacg ccgcatccgg caacagaacc tgtaaaccag cttataaacc
    59881 ggcagaacat ctcgacactc cgggagaaca acctatgaaa gacaatccgc tgatttcaat
    59941 ctatatgccg acctggaacc gtcaacaact ggcgattcgg gcgataaaat cggtcctgcg
    60001 ccaggactac agtaactggg agatgatcat cgtggatgat tgctccacct cctgggaaca
    60061 actacaacag tacgtcaccg ccctcaacga tccgcgtatc acttacattc acaacgacat
    60121 taacagcggg gcgtgcgcgg tacgtaacca ggcgattatg ctggcgcaag gggaatatat
    60181 caccgggatc gatgatgatg atgaatggac acccaaccgt ctgagcgtct tcctcgccca
    60241 taaacagcaa ctggtcacac acgccttttt gtacgctaac gactacgttt gccagggcga
    60301 agtctattcc caaccggcaa gtttgccact gtatcccaaa tcgccgtatt cacgccgcct
    60361 gttctacaaa cgcaatatca ttggcaatca ggtctttacc tgggcgtggc gtttcaaaga
    60421 gtgtttgttc gataccgaac tgaaagcggc acaggattac gacattttcc tgcggatggt
    60481 ggtggagtat ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc atatcaatca
    60541 cggcgaaatg cagatcacct cgtcaccgaa aaaattctcc ggatacttcc acttttaccg
    60601 taagcacaaa gacaaatttg accgcgccag caaaaaatat cagctgttta ccctctacca
    60661 gatccgcaat aaacgcatga cctggcgcac cttgttgacg ctgctgtcgg tgcgtaacgg
    60721 caaacgtctg gctgatggta ttcgggggcg ctaaagatgc tggaagatct gcgcgccaac
    60781 agctggagtt tacgcccgtg ctgcatggtt cttgcctatc gcgccgctca tttttgttcg
    60841 gtgtggcgca aaaagaacgt cctcaacaat ctttgggcgg ccccgctgct ggtgctgtat
    60901 cgcattatca ccgaatgctt tttcggttat gaaatccagg ccgccgcgac cattggccgc
    60961 cgtttcacta tccatcacgg ttacgccgta gtgatcaata aaaacgtggt agcgggggat
    61021 gatttcacca ttcgccacgg cgtcactatc ggcaatcgtg gtgccgataa catggcatgt
    61081 ccacacattg gcaacggcgt cgaactcggt gccaacgtca ttattcttgg tgatatcacg
    61141 cttggtaaca acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt cccggacaac
    61201 gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt ctgcaattca
    61261 atgtgcgact ggcggaaggc ggggcggcag gtgtggcgtt agatctccac cagcgcgcgc
    61321 tgcaacaggg gctggcgtca cattttgtct acggctacgg caaaggcggc aaagagagcg
    61381 tcagccatca aaactatccg caggtcatca aacatacgcc gcggatgacc gcgatggcga
    61441 acattgcgtt gtttcgtctg cttaatcgcg atctgttcgg caatttcaat gagttatatc
    61501 gcaccattac tcgcacaccg ggtccggtgg tcctgcattt tcatgtgctg cacagctact
    61561 ggttgaatct taagagcgtg gtgcgctttt gcgaaaaagt gaaaaaccat aaaccggatg
    61621 tcactctggt ctggacgtta cacgatcact ggagcgttac cggacgctgc gcctttaccg
    61681 acggttgcga aggctggaaa acgggctgcc agaaatgccc gaccttaaat aattatccgc
    61741 cggtgaagat tgatcgcgca caccaacggg tggcgggcaa acggcagtta ttcagcgaga
    61801 tgctggcgct gggctgtcag tttatttccc ccagccagca tgtggcagac gctttcaata
    61861 gtctgtacgg tccagggcgt tgccggatta tcaataacgg tattgatatg gcaaccgaag
    61921 cgattctggc ggacttgcct ccggtgcgcg aaacccaggg caagccgaaa atcgcggtgg
    61981 tggcgcatga cctgcgttac gacggcaaaa ctaaccagca actggtacgc gagatgatgg
    62041 cgctgggcga caaaatcgaa ctgcatactt tcggtaagtt ctcgccgttc accgctggta
    62101 acgttgttaa tcacggcttt gaaactgaca aacgtaaact gatgagcgcg ctcaaccaga
    62161 tggatgcgct ggtgttcagt tctcgcgtcg ataactaccc gctgattttg tgtgaggcgt
    62221 tatcgattgg cgtgccggtg attgccaccc atagcgacgc ggcgcgggaa gtgctgcaaa
    62281 aatccggcgg taaaaccgtc agcgaagaag atgtgctgca actggtgcag tcaagcaaac
    62341 cggaaatcgc acaggcgata tttggtacca cgctggctga gttcagccag cgcagccgcg
    62401 ccgcctacag tggacaacag atgctggagg agtatgtcaa cttctatcag aatctgtagc
    62461 tacctgctgc tgccgctgat ctacctgctg gttaacgtca aaatcgccca gcttggcgaa
    62521 agttttccca tcaccatcgt cactttttta cctgtcttgt tactgctgtt tttagaacgc
    62581 atcagcgtta aaaaattgat gattgcctta gggattggtg cgggactcac ggcgtttaac
    62641 tacctgtttg gtcagtcgtt ggatgccggt aaatacgtca cttcaactat gctgtttgtc
    62701 tatattgtga tcattattgg catggtgtgg agtattcgct ttaaaacgat ttcgccccac
    62761 aaccaccgca agatattacg tttcttttat ctggtggtcg ggctggtggt ggtgctggcg
    62821 gcggtggaga tggcgcaaat tatcctcacc ggcggtagca gtattatgga gtcgatttcg
    62881 aaatatctga tttacagcaa cagctatgtg ctgaatttca ttaaattcgg cggcaagcgc
    62941 acgacagcac tttatttcga accggcattt ttcgctctgg cattaatctc aatttggctc
    63001 agcatcaaac agtttggtat caaaacgcct aaaaccgatg ctatgattct tgcagggata
    63061 atattatccg gatcgttttc aggggttatg acctttatcc tgttttattt gctggagtgg
    63121 gcatttcaat atctgaataa agaggcgatt aagaaaaagt taccgttagc attgatttct
    63181 ctggctgtat tcctggttgg tgtggtaatc gcgtttcctt atatttccac ccgtctgggc
    63241 gatttaggta cggaaggatc gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc
    63301 ggttattctt tgacccatat tgacggtgta gtcagatttg gctcacttta tgaatatgtc
    63361 gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat
    63421 ctgctgatta tttatttttc ctggttcgca gtgtttttat cgctgtggta catggggaaa
    63481 gtgataaaaa tgatgatcaa cgcttttggt gataaccgca attttcgcgt gcaattatat
    63541 ctttttactc cggtatcgct gttttttacc ggttcgatat ttagcccgga atatgcattt
    63601 ttaatcgtct gtccgtttat tttgcgaaaa gcgttaaata ttacgaggta agaataagaa
    63661 catgttgctt agcataatca ctgtcgcgtt tcgtaacctc gcagggatag tcaaaacaca
    63721 tgcctcgttg gcgcatctgg cgcaggctga cgatatcagc ttcgaatgga ttgttgtcga
    63781 tggcggttcc aatgacggca cccgtgagta tctggaaaat ctcaatggta tctataacct
    63841 acgctttgtc agcgagccag ataacggtat ctacgacgcc atgaacaaag gcattgcgat
    63901 ggcgcatggc aagttcgcgt tgtttctcaa ctcaggcgat atttttcatc aggatgccgc
    63961 aaattttgtc cgtaagttaa aagtgcaaaa agataacatg atgatcaccg gcgatgcgct
    64021 gctggatttt ggcgacgggc ataaaattaa acgtagcgcc aaaccgggtt ggtatattta
    64081 tcacagcctg cccgccagtc atcaggcgat atttttcccg gtgtccggct tgaaaaaatg
    64141 gcgttatgac ctggaatata aagtttcttc cgactacgcg ctggcagcca aaatgtataa
    64201 agccggttat gcatttaaaa aactcaatgg cctggtgtct gaattttcta tgggtggggt
    64261 atcaaccacc aataatatgg aattgtgtgc tgacgccaaa aaagtccaac ggcaaatatt
    64321 acatgtgcct ggcttctggg cagaattatc ctggcattta cgccaacgta ctacctcaaa
    64381 gacgaaagcc ttatataaca aaagctgaat ataagggaaa acgatgcaag atttaagcgg
    64441 tttctcggta ccgaaagggt tccggggtgg caacgctatt aaagtgcaat tgtggtgggc
    64501 agtacaggca acaatatttg cctggtcgcc acaagtattg tatcgctggc gggcattttt
    64561 attacgttta ttcggcgcaa aaataggaaa aaacgtagtt attcgtccgt cagtaaaaat
    64621 tacctatccg tggaaattaa ccttaggtga ttacgcgtgg gtcggcgatg acgtcaattt
    64681 atataccctc ggtgaaataa ccattggcgc acattcggtg atatcgcaaa aaagttattt
    64741 atgcaccggt agccacgacc atgcaagtca acatttcacc attaacgcca cgcctattgt
    64801 gattggcgag aaatgctggc tggcaaccga tgtctttgtt gccccaggcg tcacgatcgg
    64861 caacggcacc gtcgtgggtg cacgaagcag tgtttttaaa tcgcttccgg caaatgtggt
    64921 ttgccggggg aatcccgcag tggtgatacg cgaacgcgtt gaaactgaat aaattcaaaa
    64981 aatacagagg aataagacat gtcaaaagtc gctctcatca ccggtgtaac cggacaagac
    65041 ggttcttacc tggcagagtt tctgctggaa aaaggttacg aggtgcatgg tattaagcgt
    65101 cgtgcatcgt cattcaacac cgagcgcgtg gatcacattt atcaggatcc gcacacctgc
    65161 aacccgaaat tccatctgca ttatggcgac ctgagtgata cctccaacct gacgcgtatt
    65221 ttgcgtgaag tgcagccgga tgaagtgtac aacctgggcg caatgagcca cgttgcggtt
    65281 tcttttgagt caccggaata taccgcagac gtcgatgcga tgggtacgct gcgcctgctg
    65341 gaggcgatcc gcttcctcgg tctggaaaag aaaacccgtt tctatcaggc ttctacttct
    65401 gaactgtacg gtctggtgca ggaaattccg cagaaagaga ccacgccgtt ctacccacgc
    65461 tctccgtatg cggtcgccaa actgtacgcc tactggatca ccgttaacta ccgcgaatcc
    65521 tacgacatgt acgcctgtaa cggtattctc ttcaaccatg aatccccgcg ccgtggcgag
    65581 actttcgtta cccgcaaaat cacccgcgca atcgccaaca tcgcccaggg gctggagtcg
    65641 tgcctgtatc tcggcaatat ggattccctg cgtgactggg gccacgccaa agactacgta
    65701 aaaatgcagt ggatgatgct gcaacaggaa caaccggaag atttcgttat cgcgaccggc
    65761 gttcagtact ccgtacgtca gttcgtggaa atggcggcag cacagttggg catcaaactg
    65821 cgctttgaag gcacgggtgt tgaagagaag ggcattgtgg tttccgtcac cgggcatgac
    65881 gcgccgggcg ttaaaccggg cgatgtgatt atcgccgttg acccgcgtta cttccgtccg
    65941 gcggaagttg aaacgctgct cggcgacccg accaaagcgc acgaaaaact gggctggaaa
    66001 ccggaaatca ccctcagaga gatggtgtct gaaatggtgg ctaatgacct cgaagcggcg
    66061 aaaaaacact ctctgctgaa atctcacggc tacgacgtgg cgatcgcgct ggagtcataa
    66121 gcatgagtaa acaacgcatt tttattgctg gccatcgcgg gatggtcggt tccgccatca
    66181 ggcggcagct cgaacagcgc ggtgatgtag agctggtatt acgcacccgc gacgagctga
    66241 acctgctgga cagccgcgcg gtgcatgatt tctttgccag cgaaagcatt gaccaggtct
    66301 atctggcggc ggcgaaagtg ggcggcattg ttgccaacaa cacgtatccg gcggatttca
    66361 tctaccagaa catgatgatt gagagcaaca tcattcacgc cgcgcatcag aacgacgtga
    66421 acaaactgct gtttctcgga tcgtcctgca tctacccgaa actggcaaaa cagccgatgg
    66481 cagaaagcga gttgttgcag ggcacgctgg agccgactaa cgagccttat gctattgcca
    66541 aaatcgccgg gatcaaactg tgcgaatctt acaatcgcca gtacggacgc gattaccgct
    66601 cagtcatgcc gaccaacctg tacgggccgc atgacaactt ccacccgagt aactcccatg
    66661 tgatacctgc attgctgcgt cgcttccacg aggcgacggc acagaatgcg ccggacgtgg
    66721 tggtatgggg cagcggtaca ccgatgcgtg aattcctgca cgtcgatgat atggcggcgg
    66781 cgagcattca tgtcatggag ctggcgcacg aagtctggct ggaaaacacc cagccgatgc
    66841 tgtcgcacat taacgtcggc acgggcgttg actgcactat ccgcgagctg gcgcaaacca
    66901 tcgccaaagt ggtgggttac aaaggtcggg tggtttttga tgccagtaaa ccggatggta
    66961 caccgcgcaa actgctggat gtgacgcgcc tgcatcagct tggctggtat cacgaaatct
    67021 cactggaagc ggggcttgcc agcacttacc agtggttcct tgagaatcaa gaccgctttc
    67081 gggggtaatg atgtttttac gtcaggaaga ctttgccacg gtagtgcgct ccacaccgct
    67141 tgtctctctc gactttattg tcgagaacag tcgcggcgag tttctgcttg gcaaaagaac
    67201 caaccgcccg gcgcagggtt actggtttgt gccgggaggg cgcgtgcaga aagacgaaac
    67261 gctggaagcc gcatttgagc ggctgacgat ggcggaactg gggctgcgtc tgccgataac
    67321 agcaggccag ttttacggtg tctggcagca cttttatgac gataacttct ccggcacgga
    67381 tttcaccact cactatgtgg tgctcggttt tcgcttcaga gtagtggaag aagagctgtt
    67441 actgccggat gagcagcatg acgattaccg ctggctgacg ccggacgcac tgctcgccag
    67501 tgacaatgtt cacgctaaca gccgcgccta ttttctcgct gagaagcgtg ccggagtacc
    67561 cggattatga aaatactggt ctacggcatt aactactcgc cggagttaac cggcatcggc
    67621 aaatacaccg gcgagatggt ggaatggctt gcggcgcaag gtcatgaggt gcgggttatt
    67681 accgcaccgc cttactaccc gcaatggcag gtgggcgaga actattccgc ctggcggtac
    67741 aaacgagaag agggggccgc cacggtgtgg cgctgcccgc tgtacgtgcc aaaacagccg
    67801 agcaccctga aacgcctgtt gcatctcggc agttttgccg tcagcagttt tttcccgttg
    67861 atggcgcaac gtcgctggca gccggatcgc attattggcg tggtgccaac gctgttttgc
    67921 acgccgggaa tgcgcctgct ggcgaaactc tctggggcgc gcacggtgct ccatattcag
    67981 gattacgaag tggacgccat gctggggctg ggccttgccg gaaaaggtaa aggcggcaaa
    68041 gtggcacagc tggcaacggc gttcgaacgt agcggactgc ataacgtcga taacgtctcc
    68101 acgatttcgc gttcgatgat gaataaagct atcgaaaaag gcgtggcggc ggaaaacgtc
    68161 atcttcttcc ccaactggtc ggaaatcgcc cgttttcagc atgttgcaaa tgccgatgtt
    68221 gatgcccttc gtaaccagct tggcctgccg gataacaaaa aaatcattct ttactccggc
    68281 aatattggtg aaaagcaggg gctggaaaac gttattgaag ctgccgatcg cctgcgcgat
    68341 gaaccgctga tttttgccat tgtcgggcag ggcggcggca aagcgcggct ggaaaaaatg
    68401 gcgcagcagc gtggactgcg caacatgcaa ttttttccgc tgcaatcgta tgacgcttta
    68461 cccgcactgc tgaagatggg cgattgccat ctggtggtgc aaaaacgcgg cgcggcagat
    68521 gccgtattgc cgtcgaaact gaccaatatt ctggcggtag gcggtaacgc ggtgattacc
    68581 gcagaagccc acaccgaact gggacagctt tgcgaaacct ttccgggcat tgcggtttgc
    68641 gtggaaccgg aatcggtcga ggcgctggtg gcggggattc gtcaggcgct cctgctgccc
    68701 aaacacaaca cggtggcacg tgaatatgcc gaacgcacgc tcgataaaga gaacgtgtta
    68761 cgtcaattta taaatgatat tcggggataa ttatggcgca gtcgaaactc tatccagttg
    68821 tgatggcagg tggctccggt agccgcttat ggccgctttc ccgcgtactt taccccaagc
    68881 agtttttatg cctgaaaggc gatctcacca tgctgcaaac caccatctgc cgcctgaacg
    68941 gcgtggagtg cgaaagcccg gtggtgattt gcaatgagca gcaccgcttt attgtcgcgg
    69001 aacagctgcg tcaactgaac aaactcaccg agaatattat tctcgaaccg gcagggcgta
    69061 acactgcacc tgccattgcg ctggcggcgc tggcggcaaa acgtcatagc ccggagagcg
    69121 acccattaat gctggtgctg gcggcggatc atgtgattgc cgatgaagac gcttttcgca
    69181 ccgccgtgcg taatgccatg ccgtatgccg aagcgggcaa gctggtgacc ttcggcattg
    69241 tgccggatct acctgaaacc ggatatggct atattcgtcg cggtgaagtg tctgcgggtg
    69301 aacaggatac ggtggccttt gaagtggcgc agtttgtcga aaaaccgaat ctggaaaccg
    69361 cgcaggccta tgtggcaagc ggcgaatatt actggaacag cggtatgttt ctgttccgcg
    69421 ccggacgcta tctcgaagaa ctgaaaaaat atcgcccgga tattctcgac gcctgtgaaa
    69481 aagcgatgag cgccatcgat ccggatctcg attttattcg tgtggatgaa gaagcgtttc
    69541 tcgcctgccc ggaagagtcg gtggattacg cagtcatgga acgtacggca gatgctgttg
    69601 tggtgccgat ggatgcgggc tggagcgatg tcggttcctg gtcctcttta tgggagatca
    69661 gcgcccacac cgccgagggc aacgtttgcc acggcgatgt gattaatcac aaaactgaaa
    69721 acagctatgt gtatgccgaa tctggcctgg tcaccaccgt cggggtgaaa gatctggtgg
    69781 tggtgcagac caaagatgcg gtgctgattg ccgaccgtaa cgcggtgcag gatgtgaaaa
    69841 aagtggtcga gcagatcaaa gccgatggtc gccatgagca tcgggtgcat cgcgaagtgt
    69901 atcgtccgtg gggcaaatat gactctatcg acgcgggcga ccgctaccag gtgaaacgca
    69961 tcaccgtgaa accgggcgaa ggcttgtcag tacagatgca ccatcaccgc gcggaacact
    70021 gggtagtggt cgcgggaacg gcaaaagtca ctatcgacgg tgatatcaaa ctccttggtg
    70081 aaaacgagtc catttatatt ccgctggggg cgacgcattg cctggaaaac ccggggaaaa
    70141 ttccgctcga tttaattgaa gtgcgctccg gctcttatct tgaagaggat gatgtggtgc
    70201 gcttcgcgga tcgctacgga cgggtgtaaa cgtcgcatca ggcgctgttt gtcggatgcg
    70261 gcgtgaacgc cttatccgac ctacggttcg ggtttgtagg cctgataaga cgcggcagcg
    70321 tcgcatcagg caatgaatgc gaaaccgcga tgtaaataac gacaaaaaca aaattggccg
    70381 tttcggtcag ggccaactat tgcctgaaaa agggtaacga tatgaaaaaa ttaacctgct
    70441 ttaaagccta tgatattcgc gggaaattag gcgaagaact gaatgaagat atcgcctggc
    70501 gcattggtcg cgcctatggc gaatttctca aaccgaaaac cattgtgtta ggtggtgatg
    70561 cccgcctcac cagcgaaacc ttaaaactgg cgctggcgaa aggtttacag gatgcgggcg
    70621 tcgatgtgct ggatattggt atgtccggca ccgaagaaat ctatttcgcc acgttccatc
    70681 tcggcgtgga tggcggcatt gaagttaccg ccagccataa cccgatggat tacaacggta
    70741 tgaaactcgt gcgcgagggg gcccgcccga tcagcgggga taccggactg cgcgacgtcc
    70801 agcgtctggc agaagccaac gactttcctc ccgttgatga aaccaaacgc ggtcgctatc
    70861 agcaaatcaa tctgcgtgac gcttacgttg atcacctgtt cggttatatc aacgtcaaaa
    70921 acctcacgcc gctcaagctg gtgatcaact ccgggaacgg cgcagcgggt ccggtggtgg
    70981 acgccattga agcccgcttt aaagccctcg gcgcaccagt ggaattgatc aaagtgcaca
    71041 acacgccgga cggcaatttc cccaacggaa ttcctaaccc gctgctgccg gaatgccgcg
    71101 acgacacccg caatgcggtc atcaaacacg gcgcggatat gggcattgcc tttgatggcg
    71161 attttgaccg ctgttttctg tttgacgaaa aagggcagtt tatcgagggc tactacattg
    71221 tcggcctgct ggcagaagcg ttcctcgaaa ataatcccgg cgcgaagatc atccacgatc
    71281 cgcgtctctc ctggaacacc gttgatgtgg tgactgccgc aggcggcacc ccggtaatgt
    71341 cgaaaaccgg acacgccttt attaaagaac ggatgcgcaa ggaagacgcc atctacggtg
    71401 gcgaaatgag cgcccaccat tacttccgtg atttcgctta ctgcgacagc ggcatgatcc
    71461 cgtggctgct ggtcgccgaa ctggtgtgcc tgaaagggaa aacgctgggc gaactggtgc
    71521 gtgaccggat ggcagcattt ccggcaagcg gtgagatcaa cagcaaactg gcggaccctg
    71581 ttgaggcgat taaccgcgtc gaacagcatt ttagccgcga ggcgctggcg gtggatcgca
    71641 ccgatggcat cagcatgacc tttgccgact ggcgctttaa cctgcgcacc tccaacaccg
    71701 aaccggtggt gcgcctgaat gttgaatcgc gcggtgatgt gccgctgatg gaagcgcgaa
    71761 cgcgaactct gctgacgttg ctgaacgagt aatgtcggat cttcccttac cccactgcgg
    71821 gtaaggggct aataacagga acaacgatga caaatctaaa aaagcgcgag cgagcgaaaa
    71881 ccaatgcatc gttaatctct atggtgcaac gcttttcaga tatcaccatc atgtttgccg
    71941 gactatggct ggtttgcgaa gtcagcggac tgtcattcct ctacatgcac ctgttggtgg
    72001 cgctgattac gctggtggtg ttccagatgc tgggcggcat caccgatttt tatcgctcat
    72061 ggcgcggtgt tcgggcagcg acagaatttg ccctgctgct acaaaactgg accttaagcg
    72121 tgattttcag cgccgggctg gtggcgttca acaatgattt cgacacgcaa ctgaaaatct
    72181 ggctggcgtg gtatgggctg accagtatcg gactggtggt ttgccgttcg tgtattcgca
    72241 ttggggcggg ctggctgcgt aatcatggct ataacaagcg catggtcgcg gtggcggggg
    72301 atttagccgc cggacaaatg ctgatggaga gcttccgtaa tcagccgtgg ttagggtttg
    72361 aagtggtggg cgtttaccac gacccgaaac cgggtggcgt ttctaacgac tgggcgggca
    72421 atctgcaaca gctggtcgag gatgcgaaag cgggcaagat tcataacgtc tatatcgcga
    72481 tgcaaatgtg cgacggcgca cgcgtgaaaa aactggtcca tcaattggcg gacaccacct
    72541 gttcggtgct gctgatcccc gatgtcttta ccttcaacat tctccattcc cgtatcgaag
    72601 agatgaacgg cgtaccggtg gtgccgctgt atgacacgcc gctttccggg gttaaccgcc
    72661 tgctcaaacg tgcggaagat attgtgctgg cgacgcttat cctgctgctg atctccccgg
    72721 tgctgtgctg cattgcgctg gcggtgaaac tgagctcacc ggggccggtt attttccgcc
    72781 agactcgcta cggcatggac ggcaagccga tcaaagtgtg gaagttccgt tccatgaaag
    72841 tgatggagaa cgacaaagtg gtgacccagg cgacgcagaa cgatccgcgc gtcaccaaag
    72901 tggggaactt tctgcgccgc acctcgctgg atgaattgcc gcagtttatc aatgtgctga
    72961 ctggcgggat gtcgattgtc gggccacgtc cgcacgcggt ggcgcataac gaacagtatc
    73021 gccagctcat tgaaggctac atgctgcgcc ataaagtgaa accgggcatt accggctggg
    73081 cgcagattaa cggctggcgc ggcgaaaccg acacactgga gaaaatggaa aaacgcgtcg
    73141 agttcgacct tgagtacatc cgcgaatgga gcgtctggtt cgatatcaaa atcgttttcc
    73201 tgacggtatt caagggcttc gttaacaaag cggcatattg atatgaactt acgtgaaaaa
    73261 accatcagcg gcgcgaagtg gtcggcgatt gccacggtga tcatcatcgg cctcgggctg
    73321 gtgcagatga ccgtgctggc gcggattatc gacaaccacc agttcggttt gcttaccgtg
    73381 tcgctggtga tcatcgcgct ggcagatacg ctttctgact tcggcatcgc taactcgatt
    73441 attcagcgaa aagaaatcag ccaccttgaa ctcaccacgc tgtactggct gaacgtcggg
    73501 ctggggatcg tggtgtgcgt ggcggtgttt ttgttgagtg ataccattgg cgacgtgctg
    73561 aataacccgg acctggcacc gttgattaaa acattgtcgc tggcgtttgt ggtgatcccc
    73621 cacgggcagc agttccgcgc gttgatgcag aaggagctgg agttcaacaa aatcggcatg
    73681 atcgaaacca gtgcggtgct ggcgggcttc acttttacgg tggttagcgc ccatttctgg
    73741 ccgctggcga tgaccgcgat cctcggttat ctggtcaata gtgcggtaag aacgctgctg
    73801 tttggctact ttggccgcaa aatttatcgc cccggtctgc atttctcgct ggcgtcggtg
    73861 gcaccaaact tacgttttgg tgcctggctg acggcggaca gcatcatcaa ctatctcaat
    73921 accaaccttt caacactcgt gctggcgcgt attctcggcg cgggcgtggc agggggatac
    73981 aacctggcgt acaacgtggc cgttgtgccg ccgatgaaac tgaaccccat catcacgcgc
    74041 gtgttgtttc cggcatttgc caaaattcag gacgacaccg aaaagctgcg cgtcaacttc
    74101 tacaagctgc tgtcggtggt ggggattatc aactttccgg cgctgctggg gctgatagtg
    74161 gtgtcgaata actttgtacc gctggtattt ggtgagaagt ggaacagcat tattccggtg
    74221 ctgcaattgc tgtgtgtggt ggggctgctg cgctcagtgg ggaacccgat tggttcgctg
    74281 ctgatggcga aagcgcgggt cgatatcagc tttaaattca acgtattcaa aacctttctg
    74341 tttattccgg cgattgttat tggtgggcag atggcgggcg caatcggcgt cacgcttggc
    74401 ttcctgctgg tgcagattat caacaccatt ctgagctatt tcgtgatgat taaaccggtg
    74461 ctcggttcca gttatcgcca gtacatcctg agtttgtggc tgccgtttta tctctcgctg
    74521 ccgacgctgg tggtcagtta tgcgctgggc attgtgctga aagggcaact ggcgctgggg
    74581 atgctgctgg cggtgcaaat agccacgggc gtgctggcgt ttgtggtgat gattgtgctg
    74641 tcgcgccatc cgctggtggt ggaagtgaag cgtcagtttt gtcgcagcga aaaaatgaaa
    74701 atgcttttac gggcggggtg aatggttgtt ttcggttccg gcaacggttg ccggatgcgg
    74761 cgtaaaacgc cttatccgtc ctggggggct gggcttggat cttgtaggcc tgataagacg
    74821 cgccagcgtc gcatcaggca ctgaatgccg gatgcggcgt gaacgcctta tccggcctac
    74881 ggttctgtgc tcggatcttg taggcctgat aagacgcgtc agcgtcgcat caggcactga
    74941 atgccggatg cggcgtaaaa cgccttatcc gtcctacggc tctgtgctcg ggtcttgtag
    75001 gcctgataag acgcgtcagc gtcgcatcag gcactgaatg ccggaagcgg cgtgaacgcc
    75061 ttatccgtcc tacggttctg ggcttggatc ttgtaggcct gataagaggc gtcagcgtcg
    75121 catcaggtac tgaatgccgg atgcggcgtg aacgccttat ccgtcctacg gttctgggct
    75181 tggatcttgt aggcctgata agacgcgtca gcgtcgcatc aggcactgaa tgccggatgc
    75241 ggcgtgaacg ccttatccgt cctacggttc tgtgctcgga tcttgtaggc ctgataagac
    75301 gcgttagcgt cgcatcaggc aaaaaacggc aacgaacaca acaaacacag cctcacctgg
    75361 catcaaattt atgaggaaaa aatgaaatta cttattctgg gcaaccacac ttgcggcaat
    75421 cgtggcgata gcgccatcct gcgtggctta cttgatgcca tcaatatcct caatccacat
    75481 gccgaagtgg acgtgatgag ccgctatccg gtcagttctt cctggctgct caaccgcccg
    75541 gtaatgggcg atccgctgtt cctgcaaatg aaacaacaca acagcgcggc gggcgttgtc
    75601 gggcgtgtta aaaaagtcct ccgtcgccgc taccagcacc aggtattgct ctcacgcgtc
    75661 accgacactg gcaagctgcg taatatcgcc atcgcccagg gattcactga cttcgtgcga
    75721 ctgctgtcag gttacgacgc cattattcag gtcggcggat cgttttttgt cgatctctac
    75781 ggcgtgccgc agtttgaaca tgcgctttgc actttcatgg cgaaaaagcc gctgtttatg
    75841 attggtcaca gcgtcggccc cttccaggat gagcaattta accaactggc gaactatgtc
    75901 tttggtcact gcgacgcgct gatcctgcgc gaatcggtca gccttgattt gatgaaacgc
    75961 agcaatatca ccactgcaaa agtggaacat ggcgtcgata ccgcgtggct ggtcgatcac
    76021 cacattgaag acttcaccgc cagctatgct gttcaacact ggctggatgt tgccgcacaa
    76081 cagaaaacgg tggcaattac cctgcgcgaa ctggcaccgt tcgacaaacg tctcggcacc
    76141 actcaacaag cgtatgaaaa agcctttgcc ggggtggtca atcgcattct cgatgaaggg
    76201 tatcaggtga ttgcgctctc cacctgtacg ggcatcgaca gttataacaa agacgaccgc
    76261 atggtggcgc tcaatctgcg ccagcacatc agcgatcctg cccgttatca cgtagtgatg
    76321 gatgaactca acgatctgga aatgggcaaa attctggggg cctgtgaact taccgtcggt
    76381 acgcgcctgc actctgctat tatctcgatg aactttgcca ctccggcgat tgccatcaac
    76441 tacgaacata aatccgccgg gatcatgcaa caactggggc taccagaaat ggcgattgat
    76501 atccgtcatt tattagacgg cagcctgcaa gcgatggttg cggatacctt aggccagctt
    76561 ccggcactga acacgcgact taacgaagcc gtcagtcgcg aacgtcagac gggaatgcag
    76621 atggtgcaat ctgtgcttga acgcatcggg gaggtgaaat gaaggtcggc ttctttttac
    76681 tgaaatttcc gttgtcgtcg gaaaccttcg ttcttaacca aatcactgcg tttattgata
    76741 tgggatttga ggtggagatt atcgcgctgc aaaaaggcga cacacaaaac acccacgcgg
    76801 catggacgaa atacaacctt gctgccagaa cccgctggtt acaggacgaa cctacgggca
    76861 aagtggcgaa actgcgccac cgcgccagcc agaccttatg cggcattcat cgtaaaaata
    76921 cctggcaggc gctcaacctc aaacgctatg gcgccgagtc gcggaacctg attttgtctg
    76981 ctatttgcgg tcaggtcgca acaccgtttc atgccgatgt ctttatcgct cactttggtc
    77041 cggcaggggt aaccgcagca aaactccgcg aactgggtgt cattcgcggc aaaattgcca
    77101 ctatcttcca cggtattgat atctccagtc gggaggtgct caaccattac actcccgaat
    77161 atcagcaact gttccgccgt ggcgacctga tgttaccgat aagcgatctg tgggccggaa
    77221 ggctgcaaaa aatgggctgc ccgagggaaa aaatcgccgt atcgcgtatg ggcgtggaca
    77281 tgacgcgctt tagcccgcgt ccggtgaaag cgcctgcaac gccgctggaa atcatttccg
    77341 tcgcacgctt aaccgagaaa aaaggcctgc atgtggcgat cgaagcctgc cgtcagttga
    77401 aagagcaggg cgtggcattt cgctatcgca tcctcggcat tggcccgtgg gaacgacgcc
    77461 tgcgcaccct catcgaacaa tatcaactgg aagatgtgat agagatgccg ggctttaaac
    77521 cgagccatga agtgaaggcg atgctcgacg acgcggatgt cttcctgttg ccatcggtta
    77581 caggtgcgga cggcgatatg gaaggtattc cggtggcact aatggaagcg atggcggtcg
    77641 gcattccggt ggtttctact ctgcatagcg gaataccgga actggtggag gccgataaat
    77701 ccggctggct ggtgcctgag aacgatgctc gcgcactggc gcaacgactg gcggcgttta
    77761 gccaactgga caccgacgaa ctggctccgg ttatcaaacg tgctcgcgaa aaagtcgaac
    77821 acgattttaa ccagcaggtg atcaatcgag aactcgccag cttgctgcag gctttataga
    77881 ggagaacgta tgccatttaa aaaactctcc cgacgcacct tcctgacggc aagctcggcg
    77941 cttgccttcc tccatacccc cttcgcccgc gcacttccag cccgacaaag cgttaatatc
    78001 aacgattaca atccgcacga ctggatcgcc tcatttaaac aagccttcag tgaagggcaa
    78061 acggtcgtcg tgcctgccgg attggtttgt gacaatatca acaccggcat cttcattcct
    78121 cctggaaaaa ccttacacat ccttggaagc ctgcgcggca acggcagagg gcgatttgtc
    78181 ttacaggacg gcagccaggt gacaggggag gagggcggca gtatgcataa catcaccctg
    78241 gatgtgcgcg gttctgactg catcatcaaa gggttggcga tgagcggttt tggcccggta
    78301 acgcagattt atatcggcgg caaaaataaa cgcgtcatgc gcaacttgac catcgataac
    78361 ctgaccgtca gccacgctaa ttacgccatt ttacgccagg gatttcataa ccagattatc
    78421 ggtgccaaca tcaccaactg taagttcagc gacttacagg gcgacgccat tgaatggaat
    78481 gtggcgatta acgaccgcga tattttgata tccgaccacg ttattgagcg catcaactgt
    78541 accaacggca aaatcaactg gggcatcggc ataggccttg cgggaagcac ttacgataac
    78601 aactatccgg aagaccaggc agtgaaaaac tttgtcgtgg cgaatatcac gggatcagat
    78661 tgtcgacagt tgatacatgt tgaaaatggt aaacattttg ttattcgtaa tatcaaagcc
    78721 cgcaatatca cgccggattt cagtaagaaa gcaggtattg ataacgccac ggtcgctatt
    78781 tacggttgtg acaatttcgt gattgataat attgaaatga ttaatagcgc cgggatgtta
    78841 attggctatg gggtaattaa aggcaaatat ctctcgatac cgcaaaattt ccgagtgaat
    78901 aatattcaac tggataacac tcaccttgct tataaattgc gcggcatcca aatctccgcc
    78961 gggaatgctg tctcctttgt ggcgctgact aacattgaga tgaagcgtgc ctcgctggag
    79021 cttcacaata aaccgcaaca tctttttatg cgaaatatca aagtgatgca ggaatcctct
    79081 gttggacccg cattgagcat gaacttcgac atgcgcaaag acgttcgcgg cgtctttatg
    79141 gcgaaaaaag aaacactgct gtctcttgca aatgttcatg cgatgaatga aagagggcaa
    79201 agctccgtcg atatcgacag ggttaatcac catattgtta atgtggaaaa gattaacttt
    79261 agattgccag aacggaggga gtagatttgc gaccattcat aaacttactg gatataatgt
    79321 tctaaaagat tataatcctc acactaactg ttaaagctgg ggctgggcag cattgactac
    79381 tcagggaagg tactcatctt tatcttgtgt ttcttacgta acaaccagac taaagtgatt
    79441 tatgaacgat aacgttttgc tcataggagc ttccggattc gtaggaaccc gactacttga
    79501 aacggcaatt gctgacttta atatcaagaa cctggacaaa cagcagagcc acttttatcc
    79561 agaaatcaca cagattggcg atgttcgtga tcaacaggca ctcgaccagg cgttagctgg
    79621 ttttgacact gttgtgctac tggcagcgga acaccgcgat gacgtcagcc ctacttctct
    79681 ctattatgat gtcaacgttc agggtacccg caatgtgctg acggccatgg aaaaaaatgg
    79741 cgttaaaaat atcatcttta ccagttccgt tgctgtttat ggtttgaaca aacacaaccc
    79801 tgacgaaaac catccacacg atcctttcaa ccactacggc aaaagtaagt ggcaggcaga
    79861 ggaagtgctg cgtgaatggt ataacaaagc accaacagaa cgttcattaa ccatcatccg
    79921 tcctaccgtt atcttcggtg aacgcaaccg cggtaacgtc tataacttgc tgaaacagat
    79981 cgctggcggc aagtttatga tggtgggcgc agggactaac tataagtcca tggcttatgt
    80041 tggaaacatt gttgagttta tcaagtacaa actgaagaat gttgccgcag gttatgaggt
    80101 ttataattac gttgataagc cagatctgaa catgaaccag ttggttgctg aagttgaaca
    80161 aagtctgaac aaaaagatcc cttctatgca cttgccttac ccactaggaa tgttgggtgg
    80221 atattgcttt gatatcctga gcaaaattac gggcaaaaaa tacgctgtca gctctgtgcg
    80281 cgtgaaaaaa ttctgcgcaa caacacagtt tgacgcaacg aaagtgcatt cttcaggttt
    80341 tgtggcaccg tatacgctgt cgcaaggtct ggatcgaaca ctgcagtatg aattcgttca
    80401 tgccaaaaaa gacgacataa cgtttgtttc tgagtaatgc agacgagtca gagaaaaaag
    80461 gaccaggttg gactacgatt attcggaaag aggtactgat taaattatca gtatgcggaa
    80521 ttccgagtaa tccgagtaac ccagatgctg atggtgtgta gtgttattct aaggtttaag
    80581 caaagttatg cggaactaag ctttggtata tagccataac gtttggctaa cctatggcaa
    80641 taatttacgc actatcagcc acttagaggt aggaaaagaa tgacgaattt aaaagcagtt
    80701 ataccggtag caggtcttgg gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag
    80761 atgctaccga tcgtcgacaa gccaatgatt cagtacattg ttgacgagat tgtggctgca
    80821 gggatcaaag aaatcctcct ggtaactcac gcgtccaaga acgcggtcga aaaccacttc
    80881 gacacctctt atgaattaga atctctcctt gagcagcgcg tgaagcgtca actactggcg
    80941 gaagtgcaat ctatctgccc gccgggcgtg actattatga acgtgcgtca gggcgaacct
    81001 ttaggtttag gtcactccat tttgtgtgca cgccccgcca ttggtgacaa cccatttgtc
    81061 gtggtgctgc cggacgttgt tatcgatgat gccagcgccg acccgctgcg ttacaacctt
    81121 gccgctatga ttgcgcgctt caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg
    81181 ccgggtgacc tctctgaata ctccgtcatc cagaccaaag aaccgctgga tcgtgaaggc
    81241 aaagtcagcc gcattgttga attcatcgaa aaaccggatc agccgcagac gctggactca
    81301 gacatcatgg ccgttggtcg ctatgtgctt tctgccgata tttggccgga actggaacgc
    81361 actcagcctg gtgcatgggg gcgtattcag ctgactgatg ctattgccga actggcgaaa
    81421 aaacagtccg ttgatgccat gctgatgact ggtgacagct acgactgtgg taaaaaaatg
    81481 ggctatatgc aggcgtttgt gaagtatgga ctgcgcaacc tgaaagaagg ggcgaagttc
    81541 cgtaaaggta ttgagaagct gttaagcgaa taatgaaaat ctgaccggat gtaacggttg
    81601 ataagaaaat tataacggca gtgaagattc gtggcgaaag taatttgttg cgaatattcc
    81661 tgccgttgtt ttatataaac aatcagaata acaaagagtt agcaatagga ttttcgtcaa
    81721 agttttccag gattttcctt gtttccagag cggattggta agacaattag cgtttgaatt
    81781 tttcgagtta agcgcgagtg ggtaacgctc gtcacatcgt aggcatgcat gcagtgctct
    81841 ggtagctgta aagccagggg cggtagcgtg cattaatacc tctattaatc aaactaagag
    81901 ccgcttattt cacagcatgc tctgaagtaa tatggaataa aagagtgaag atacttgtta
    81961 ctggtggcgc aggatttatt ggttctgctg tagttcgtca cattataaat aatacgcagg
    82021 atagtgttgt taatgtcgat aaattaacgt acgccggaaa cctggaatca cttgctgatg
    82081 tttctgattc tgaacgctat atttttgaac atgcggatat ttgcgatgct gctgcaatgg
    82141 cgcggatttt tgctcagcat cagccggatg cagtgatgca cctggctgct gaaagccatg
    82201 tggatcgttc aattacaggc cctgcggcat ttattgaaac caatattgtt ggtacttatg
    82261 tccttttgga agccgctcgc aattattggt ctgctcttga tggcgacaag aaaaatagct
    82321 tccgttttca tcatatttct actgacgaag tctatggtga tttgcctcat cctgacgaag
    82381 taaataataa agaacaatta cccctcttta ctgagacgac agcttacgcg cctagtagtc
    82441 cttattccgc atcaaaagca tccagcgatc atttagtccg tgcgtggaaa cgtacctatg
    82501 gtttaccgac cattgtgact aattgttcga ataactacgg tccttatcac tttccggaaa
    82561 aattgattcc actagtaatt cttaatgctc tggaaggtaa ggcattacct atttatggca
    82621 aaggggatca aattcgcgac tggctgtatg ttgaagatca tgcacgtgcg ttatataccg
    82681 tcgtaaccga aggtaaagcg ggtgaaactt ataacattgg tggacacaac gaaaagaaaa
    82741 acatcgatgt agtgctcact atttgtgatt tgttggatga gattgtaccg aaagagaaat
    82801 cttaccgcga gcaaattact tatgttgccg atcgtccggg acacgatcgc cgttatgcca
    82861 ttgatgctga gaagattggt cgcgaattgg gatggaaacc acaggaaacg tttgagagcg
    82921 ggattcggaa gacagtggaa tggtacctgt ccaatacaaa atgggttgat aatgtgaaaa
    82981 gtggtgccta tcaatcgtgg attgaacaga actatgaggg ccgccagtaa tgaatattct
    83041 ccttttcggc aaaacagggc aggtaggttg ggaactacag cgtgctctgg cacctttggg
    83101 taatttgatt gctcttgatg ttcactccac tgattattgt ggtgatttta gtaatcctga
    83161 aggtgtggct gaaaccgtca aaaaaattcg ccctgatgtt attgttaatg ctgcggctca
    83221 taccgcagta gataaggctg agtcagaacc cgaatttgca caattactca atgcgaccag
    83281 cgttgaatca attgcaaaag cggctaatga agttggggcc tgggtaattc attactcaac
    83341 tgactacgta tttccgggaa ccggtgaaat accatggctg gagacggatg caaccgcacc
    83401 gctaaatgtt tacggtgaaa ccaagttagc tggagaaaaa gcattacaag agcagtgtgc
    83461 gaagcacctt attttccgta ccagctgggt atatgcaggt aaaggaaaca attttgccaa
    83521 gacaatgtta cgtctggcaa aagagcgtga agaattagcc gttattaatg atcagtttgg
    83581 tgcgccaact ggcgcagagt tgctggctga ttgtacggca catgccattc gtgtggcact
    83641 gaataaaccg gaagtcgcag gtttgtacca tctggtagcc agtggtacca caacctggca
    83701 cgattatgct gcgctggttt ttgaagaggc gcgcaaagca ggcatacccc ttgcactcaa
    83761 caagctcaac gcagtagcaa caacagccta tcctacacca gctcgtcgtc cacataactc
    83821 tcgccttaat acagaaaaat ttcagcagaa ttttgcgctt gttttgcctg actggcaggt
    83881 tggcgtgaaa cgaatgctca acgaattatt tacgactaca gtaatttaat agtttttgca
    83941 tcttgttcgt gatgatggag caagatgaat taaaaggaat gatgaaatga aaacgcgtaa
    84001 aggtattatt ttagcgggtg gctctggtac tcgtctttat cctgtgacta tggctgtcag
    84061 taaacagctg ttacctattt atgataaacc gatgatctat tacccgcttt ctacactgat
    84121 gttagcgggt attcgcgata ttctgattat cagtacacca caggatactc ctcgttttca
    84181 ccaactgctg ggtgacgggg gccagtgggg gctaaatctt cagtacaaag tgcaaccgac
    84241 tccagatggg cttgcgcagg cgtttattat cggtgaagag tttatcggtg gtgatgattg
    84301 tgctctggtt ctcggtgata atatctttta cggtcatgat ctgccgaagt taatggatgt
    84361 cgctgttaac aaagaaagtg gtgcaacagt atttgcctat catgttaatg atcctgaacg
    84421 ctacggtgtc gttgagtttg ataaaaaagg tacggcaatt agcctggaag aaaaaccgtt
    84481 acaaccaaaa agtaattatg cggtaaccgg gctttatttc tatgataacg acgttgtcga
    84541 aatggcgaaa aaccttaagc cttctgcccg tggtgaactg gaaattaccg atattaaccg
    84601 tatttatatg gaacaggggg ggttatccgt tgccatgatg ggacgtggtt atgcatggct
    84661 ggacacgggt acacatcaaa gtcttattga agcaagcaac ttcattgcaa caattgaaga
    84721 gcgtcagggg ttaaaagttt cctgtccgga agaaattgct taccgtaaag ggtttattga
    84781 tgctgagcag gtgaaagtat tagctgaacc gctgaaaaaa aatgcttatg gtcag
//
LOCUS       DAZGTJ010000009        83153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000009 DAZGTJ010000000
VERSION     DAZGTJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.009"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHK8E_000833"
     CDS             complement(<1..593)
                     /locus_tag="ACHK8E_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0343139.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPPRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHK8E_000834"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHK8E_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP0343140.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHK8E_000835"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHK8E_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP0343141.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHK8E_000836"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHK8E_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP0343142.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHK8E_000837"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHK8E_000837"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP0343143.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHK8E_000838"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHK8E_000838"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP0343144.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHK8E_000839"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHK8E_000839"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP0343145.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHK8E_000840"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHK8E_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0343146.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHK8E_000841"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHK8E_000841"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0343147.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHK8E_000842"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHK8E_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP0343148.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHK8E_000843"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHK8E_000843"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0343149.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHK8E_000844"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHK8E_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP0343150.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHK8E_000845"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHK8E_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP0343151.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHK8E_000846"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHK8E_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP0343152.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHK8E_000847"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHK8E_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0343153.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHK8E_000848"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHK8E_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0343154.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHK8E_000849"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHK8E_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP0343155.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHK8E_000850"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHK8E_000850"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP0343156.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHK8E_000851"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHK8E_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP0343157.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHK8E_000852"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHK8E_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP0343158.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHK8E_000853"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHK8E_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP0343159.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHK8E_000854"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHK8E_000854"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP0343160.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHK8E_000855"
     CDS             complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHK8E_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP0343161.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHK8E_000856"
     CDS             complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHK8E_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP0343162.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHK8E_000857"
     CDS             complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHK8E_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP0343163.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHK8E_000858"
                     /gene_synonym="rotA"
     CDS             complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHK8E_000858"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP0343164.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHK8E_000859"
     CDS             21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHK8E_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP0343165.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23275..25818
                     /gene="nirB"
                     /locus_tag="ACHK8E_000860"
     CDS             23275..25818
                     /gene="nirB"
                     /locus_tag="ACHK8E_000860"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP0343166.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25815..26141
                     /gene="nirD"
                     /locus_tag="ACHK8E_000861"
     CDS             25815..26141
                     /gene="nirD"
                     /locus_tag="ACHK8E_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP0343167.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26268..27074
                     /gene="nirC"
                     /locus_tag="ACHK8E_000862"
     CDS             26268..27074
                     /gene="nirC"
                     /locus_tag="ACHK8E_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP0343168.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27093..28466
                     /gene="cysG"
                     /locus_tag="ACHK8E_000863"
     CDS             27093..28466
                     /gene="cysG"
                     /locus_tag="ACHK8E_000863"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP0343169.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
     gene            28717..28884
                     /gene="yhfL"
                     /locus_tag="ACHK8E_000864"
     CDS             28717..28884
                     /gene="yhfL"
                     /locus_tag="ACHK8E_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP0343170.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(28991..30076)
                     /gene="yhfS"
                     /locus_tag="ACHK8E_000865"
     CDS             complement(28991..30076)
                     /gene="yhfS"
                     /locus_tag="ACHK8E_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0343171.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(30088..31392)
                     /gene="yhfT"
                     /locus_tag="ACHK8E_000866"
     CDS             complement(30088..31392)
                     /gene="yhfT"
                     /locus_tag="ACHK8E_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP0343172.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(31404..31757)
                     /gene="yhfU"
                     /locus_tag="ACHK8E_000867"
     CDS             complement(31404..31757)
                     /gene="yhfU"
                     /locus_tag="ACHK8E_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP0343173.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(31768..32646)
                     /gene="php"
                     /locus_tag="ACHK8E_000868"
     CDS             complement(31768..32646)
                     /gene="php"
                     /locus_tag="ACHK8E_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP0343174.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(32643..33869)
                     /gene="yhfW"
                     /locus_tag="ACHK8E_000869"
     CDS             complement(32643..33869)
                     /gene="yhfW"
                     /locus_tag="ACHK8E_000869"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0343175.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(33869..35032)
                     /gene="yhfX"
                     /locus_tag="ACHK8E_000870"
     CDS             complement(33869..35032)
                     /gene="yhfX"
                     /locus_tag="ACHK8E_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP0343176.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(35116..35478)
                     /gene="yhfY"
                     /locus_tag="ACHK8E_000871"
     CDS             complement(35116..35478)
                     /gene="yhfY"
                     /locus_tag="ACHK8E_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0343177.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(35495..36400)
                     /gene="yhfZ"
                     /locus_tag="ACHK8E_000872"
     CDS             complement(35495..36400)
                     /gene="yhfZ"
                     /locus_tag="ACHK8E_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP0343178.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(36690..37694)
                     /gene="trpS"
                     /locus_tag="ACHK8E_000873"
     CDS             complement(36690..37694)
                     /gene="trpS"
                     /locus_tag="ACHK8E_000873"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP0343179.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(37687..38445)
                     /gene="gph"
                     /locus_tag="ACHK8E_000874"
     CDS             complement(37687..38445)
                     /gene="gph"
                     /locus_tag="ACHK8E_000874"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP0343180.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(38438..39115)
                     /gene="rpe"
                     /locus_tag="ACHK8E_000875"
     CDS             complement(38438..39115)
                     /gene="rpe"
                     /locus_tag="ACHK8E_000875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP0343181.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(39133..39969)
                     /gene="dam"
                     /locus_tag="ACHK8E_000876"
     CDS             complement(39133..39969)
                     /gene="dam"
                     /locus_tag="ACHK8E_000876"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0343182.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(40076..41362)
                     /gene="damX"
                     /locus_tag="ACHK8E_000877"
     CDS             complement(40076..41362)
                     /gene="damX"
                     /locus_tag="ACHK8E_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP0343183.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(41454..42542)
                     /gene="aroB"
                     /locus_tag="ACHK8E_000878"
     CDS             complement(41454..42542)
                     /gene="aroB"
                     /locus_tag="ACHK8E_000878"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP0343184.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(42599..43120)
                     /gene="aroK"
                     /locus_tag="ACHK8E_000879"
     CDS             complement(42599..43120)
                     /gene="aroK"
                     /locus_tag="ACHK8E_000879"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP0343185.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(43521..44759)
                     /gene="hofQ"
                     /locus_tag="ACHK8E_000880"
     CDS             complement(43521..44759)
                     /gene="hofQ"
                     /locus_tag="ACHK8E_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP0343186.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(44671..45075)
                     /gene="hofP"
                     /locus_tag="ACHK8E_000881"
     CDS             complement(44671..45075)
                     /gene="hofP"
                     /locus_tag="ACHK8E_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP0343187.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(45065..45505)
                     /gene="hofO"
                     /locus_tag="ACHK8E_000882"
     CDS             complement(45065..45505)
                     /gene="hofO"
                     /locus_tag="ACHK8E_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP0343188.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(45489..46028)
                     /gene="hofN"
                     /locus_tag="ACHK8E_000883"
     CDS             complement(45489..46028)
                     /gene="hofN"
                     /locus_tag="ACHK8E_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP0343189.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(46028..46797)
                     /gene="hofM"
                     /locus_tag="ACHK8E_000884"
                     /pseudo
     CDS             complement(46028..46797)
                     /gene="hofM"
                     /locus_tag="ACHK8E_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            46917..49469
                     /gene="mrcA"
                     /locus_tag="ACHK8E_000885"
     CDS             46917..49469
                     /gene="mrcA"
                     /locus_tag="ACHK8E_000885"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP0343190.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(49637..50197)
                     /gene="nudE"
                     /locus_tag="ACHK8E_000886"
     CDS             complement(49637..50197)
                     /gene="nudE"
                     /locus_tag="ACHK8E_000886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP0343191.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            50517..52652
                     /gene="igaA"
                     /locus_tag="ACHK8E_000887"
     CDS             50517..52652
                     /gene="igaA"
                     /locus_tag="ACHK8E_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP0343192.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            52717..53385
                     /gene="yrfG"
                     /locus_tag="ACHK8E_000888"
     CDS             52717..53385
                     /gene="yrfG"
                     /locus_tag="ACHK8E_000888"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP0343193.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            53396..53797
                     /gene="hslR"
                     /locus_tag="ACHK8E_000889"
     CDS             53396..53797
                     /gene="hslR"
                     /locus_tag="ACHK8E_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP0343194.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            53822..54700
                     /gene="hslO"
                     /locus_tag="ACHK8E_000890"
     CDS             53822..54700
                     /gene="hslO"
                     /locus_tag="ACHK8E_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP0343195.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(54838..56562)
                     /gene="yhgE"
                     /locus_tag="ACHK8E_000891"
     CDS             complement(54838..56562)
                     /gene="yhgE"
                     /locus_tag="ACHK8E_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP0343196.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            56941..58563
                     /gene="pckA"
                     /locus_tag="ACHK8E_000892"
     CDS             56941..58563
                     /gene="pckA"
                     /locus_tag="ACHK8E_000892"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP0343197.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            58678..58995
                     /locus_tag="ACHK8E_000893"
     CDS             58678..58995
                     /locus_tag="ACHK8E_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0343198.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            59054..59350
                     /gene="nadS"
                     /locus_tag="ACHK8E_000894"
     CDS             59054..59350
                     /gene="nadS"
                     /locus_tag="ACHK8E_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0343199.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(59382..60734)
                     /gene="envZ"
                     /locus_tag="ACHK8E_000895"
     CDS             complement(59382..60734)
                     /gene="envZ"
                     /locus_tag="ACHK8E_000895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP0343200.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(60731..61450)
                     /gene="ompR"
                     /locus_tag="ACHK8E_000896"
     CDS             complement(60731..61450)
                     /gene="ompR"
                     /locus_tag="ACHK8E_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP0343201.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            61678..62154
                     /gene="greB"
                     /locus_tag="ACHK8E_000897"
     CDS             61678..62154
                     /gene="greB"
                     /locus_tag="ACHK8E_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP0343202.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            62251..64572
                     /gene="yhgF"
                     /locus_tag="ACHK8E_000898"
     CDS             62251..64572
                     /gene="yhgF"
                     /locus_tag="ACHK8E_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP0343203.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            65010..65237
                     /gene="feoA"
                     /locus_tag="ACHK8E_000899"
     CDS             65010..65237
                     /gene="feoA"
                     /locus_tag="ACHK8E_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP0343204.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            65254..67575
                     /gene="feoB"
                     /locus_tag="ACHK8E_000900"
     CDS             65254..67575
                     /gene="feoB"
                     /locus_tag="ACHK8E_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP0343205.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            67575..67811
                     /gene="feoC"
                     /locus_tag="ACHK8E_000901"
     CDS             67575..67811
                     /gene="feoC"
                     /locus_tag="ACHK8E_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP0343206.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            68014..68895
                     /gene="rpnA"
                     /locus_tag="ACHK8E_000902"
     CDS             68014..68895
                     /gene="rpnA"
                     /locus_tag="ACHK8E_000902"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP0343207.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            68967..69086
                     /locus_tag="ACHK8E_000903"
                     /pseudo
     CDS             68967..69086
                     /locus_tag="ACHK8E_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(69115..69885)
                     /gene="bioH"
                     /locus_tag="ACHK8E_000904"
     CDS             complement(69115..69885)
                     /gene="bioH"
                     /locus_tag="ACHK8E_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP0343208.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            69923..70606
                     /gene="gntX"
                     /locus_tag="ACHK8E_000905"
     CDS             69923..70606
                     /gene="gntX"
                     /locus_tag="ACHK8E_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP0343209.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQQRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            70665..71240
                     /gene="nfuA"
                     /locus_tag="ACHK8E_000906"
     CDS             70665..71240
                     /gene="nfuA"
                     /locus_tag="ACHK8E_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP0343210.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            71601..72917
                     /gene="gntT"
                     /locus_tag="ACHK8E_000907"
     CDS             71601..72917
                     /gene="gntT"
                     /locus_tag="ACHK8E_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0343211.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(72962..75046)
                     /gene="malQ"
                     /locus_tag="ACHK8E_000908"
     CDS             complement(72962..75046)
                     /gene="malQ"
                     /locus_tag="ACHK8E_000908"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP0343212.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(75056..77449)
                     /gene="malP"
                     /locus_tag="ACHK8E_000909"
     CDS             complement(75056..77449)
                     /gene="malP"
                     /locus_tag="ACHK8E_000909"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP0343213.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            78071..80776
                     /gene="malT"
                     /locus_tag="ACHK8E_000910"
     CDS             78071..80776
                     /gene="malT"
                     /locus_tag="ACHK8E_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP0343214.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(80819..81835)
                     /gene="rtcA"
                     /locus_tag="ACHK8E_000911"
     CDS             complement(80819..81835)
                     /gene="rtcA"
                     /locus_tag="ACHK8E_000911"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP0343215.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(81839..83065)
                     /gene="rtcB"
                     /locus_tag="ACHK8E_000912"
     CDS             complement(81839..83065)
                     /gene="rtcB"
                     /locus_tag="ACHK8E_000912"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP0343216.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        20033 a        22656 c        20857 g        19607 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac ggggcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc
    19501 ataaaaaatc actgaatggc aatcagaaat cagcgatgca gaaaattagc cagcagttga
    19561 tgtccttgtt cgctgagaat actttctgga tggaattgca caccttccag atcccactgg
    19621 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc aaagcactcc
    19681 ggtaatgagt caggttccac caccagcgaa tggtagcgtg tcacggtgag tggatttgcc
    19741 agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc gtgcatgacc
    19801 tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19861 acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga gatcccggct
    19921 tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19981 tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag ttcacaaaag
    20041 tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat catggcggct
    20101 ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    20161 aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg cctgattcca
    20221 ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    20281 gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca cgttgatttc
    20341 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20401 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20461 gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc agtcgaaaat
    20521 atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa ccaacgggcc
    20581 aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20641 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20701 gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaaga cgcgctaaag
    20761 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20821 ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20881 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20941 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    21001 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    21061 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    21121 cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa cgttgataaa
    21181 gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt
    21241 gttgcgcagg ccgttatcag cttcattttt gattggcggg tttggttttt tctgctgcat
    21301 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtcgt
    21361 gttgttataa aaaccactgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21421 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21481 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21541 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21601 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21661 aaatatatgt aacctgagga taacttttta ttccgtgatc aaaatcacct cttaaaatgc
    21721 aatttagcaa ccgattgcaa taaaacattt aatcagatca caaaatcgcc taaaatcgcc
    21781 cgctcgcggc atagcccgct atgccatttt atttttgaac gcaggccaat catgactaac
    21841 agcaatcgca tcaagctcac atggattagc tttctttcct acgcactgac cggtgcgttg
    21901 gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc
    21961 agcatgagta acaccttcac cttcctcaat gccggcattt taatctctat cttcctcaac
    22021 gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg
    22081 gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc ggcggcgatg
    22141 ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct ggtaacacaa
    22201 atgtatgaag gacgtcagcg cggttcacgc ctgttattta ccgactcctt cttcagtatg
    22261 gccgggatga ttttcccaat gatcgccgcg tttctgctgg cacgtagcat tgagtggtac
    22321 tgggtttatg cctgcatcgg gctggtgtat gttgctattt ttattctgac cttcggctgt
    22381 gaattcccgg cactgggcaa acatgcgcca aaaacagatg ctccggtaga gaaagaaaag
    22441 tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt gctacatcct cggtcagtta
    22501 ggttttatct cctgggtgcc ggaatatgcc aaaggcctgg gcatgagcct gaacgacgca
    22561 ggcacgctgg taagtaactt ctggatgtcg tacatggtcg gcatgtgggc gttcagcttt
    22621 attcttcgct tctttgattt gcaacgtatc ctgatcgtac tggctggtct ggctgcgatt
    22681 ctgatgtacg tctttaacac cggaacaccg gcatatatgg cgtggtcaat tctcgccctg
    22741 ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca gcagaccaaa
    22801 gtaccgtcgc caaaactggt taactttgtc ctgacctgcg ggaccatcgg tactatgttg
    22861 acctttgtgg ttaccggccc gattgttgaa catagtggtc cgcaggcggc gctgctgacg
    22921 gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt
    22981 caccgtcagc ataacaccct gacctctcat taattgttca tgccgggcgg cactatcgtc
    23041 gtccggcctt ttcctctctt actctgctac gtacatctat ttctataaat ccgttcaatt
    23101 tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag aaaatttata
    23161 caaatcagca atatacccct taaggggtat ataaaggtga atttgattta catcaataag
    23221 cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc
    23281 aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat cgaagatctt
    23341 cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga accgcgcatc
    23401 gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga agagctgtcg
    23461 ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct
    23521 atcaccatca accgtcagga gaaggtgatt cactccagcg ccggacgtac cgttttttat
    23581 gacaagctga tcatggcgac cggttcctac ccgtggatcc cgccaattaa aggttctgat
    23641 actcaggact gctttgtcta tcgcaccatt gaagacctca acgccattga atcctgcgct
    23701 cgtcgcagca agcgcggtgc cgttgttggt ggcggcctgt taggtctgga agctgctggc
    23761 gcgctgaaaa acttaggcat tgaaacccac gttatcgaat ttgcccctat gctgatggcg
    23821 gaacagcttg atcagatggg cggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg
    23881 cgcgttcaca ccagcaaaaa cactcttgag attgtgcagg aaggtgttga agcgcgtaaa
    23941 accatgcgtt ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt ctctaccggt
    24001 atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt
    24061 atcgtcataa atgattcctg ccagacttcc gatccggata tctacgccat cggtgaatgc
    24121 gcaagctgga acaaccgtgt atttggtctg gtagcaccag gctacaaaat ggcgcaggtc
    24181 gccgttgacc atattcttgg tagcgaaaac gcctttgaag gtgcagacct tagcgccaag
    24241 ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg cacacctggc
    24301 gcacgtagct acgtttacct cgacgaaagc aaagagatct acaaacgcct tattgtcagc
    24361 gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga ctacggcaac
    24421 ctgctgcaac tggtgctgaa tgctatcgag ctgccggaaa acccggattc tctgattctg
    24481 ccggcgcact cgggtagcgg taagccgtct atcggtgttg ataaactgcc ggacagcgcg
    24541 caaatctgct cctgcttcga cgtcaccaaa ggtgatctga ttgctgccat caacaaaggc
    24601 tgccacaccg ttgcggcgct gaaagctgaa accaaagcgg gtactggctg cggtggctgt
    24661 atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat cgaagttaac
    24721 aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt gatccgcgtt
    24781 gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa
    24841 gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct ggaacgaata cattctgaaa
    24901 ccggaacata ctccgctgca ggattctaac gataacttcc tcgctaacat ccagaaagac
    24961 ggcacctact cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga agggctgatg
    25021 gcagtaggtc gtatcgcgcg tgaatttaat ctctacacca aaatcaccgg ctcccagcgc
    25081 ctggcgatgt ttggcgcaca gaaagacgat ctgccggaga tctggcgtca gctgattgaa
    25141 gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg
    25201 ggtagcacct ggtgccgcta cggcgtgggc gacagcgtcg gcctcggcgt ggaactggaa
    25261 aaccgctaca aaggtatccg gactccgcac aaaatgaagt tcggtgtctc cggctgtacc
    25321 cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccaccga aaaaggctgg
    25381 aacctgtatg tttgcggtaa cggcggcatg aaaccgcgtc atgcgaatct gttggcagcg
    25441 gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt
    25501 accgccgaca aactgacgcg taccgcgccg tggttagaaa acctcgaagg tggcatcgat
    25561 tacctgaaag cggtgatcat tgacgacaaa ctggggctga acgcacatct ggaagaagag
    25621 atggcgcgcc tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa taccccgtct
    25681 gcgcagactc gcttcaaaca cttcatcaac agcgataagc gtgacccgaa cgtgcagatg
    25741 gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc ggtaactctg
    25801 gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac atcctgcctg
    25861 aaaccggcgt ctgcgcgctg ttaggtgacg aacaagtagc aattttccgc ccgtatcaca
    25921 gcgatcaggt gtttgcgatc agcaacatcg atccgttctt cgagtccagc gtgctgtcac
    25981 gcgggctgat tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc
    26041 gttttcgctt aagtgatggc ttgtgcatgg aagacgaaca gttttccgtc aaacattacg
    26101 aagcacgagt gaaagacggc gtggtgcagc tgcgcggtta atgttttaac gggaggcgca
    26161 atgcctcccc tttttgcatg gtcctgtaat gaccttcggt atattgcagg acatttttta
    26221 aactttttgt tttatttttt tgtttttatt ttttaaagga taatcaaatg tttacagaca
    26281 ctattaataa gtgtgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc
    26341 tcggcttttg ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcctga
    26401 ttttcacgct cggtaatttg ctcgatccgt ccatacgtcc tctggtgatg ggcgcgacct
    26461 ttggtatcgc cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca
    26521 tgttcctcac ctttggggtg aaagcgggca ccatcagcca cgggcaaatg tgggcaatcc
    26581 tgccgcaaac ctggctgggt aatctggtcg gttccgtctt cgttgccatg ctctatagct
    26641 ggggcggcgg tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcaa
    26701 aaaccactgc accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt
    26761 gcctggctat ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt
    26821 ggtgtctgct ggcatttatc gcgtccggct acgagcactc catcgctaac atgacgctgt
    26881 tcgccctctc ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata
    26941 acctgctgtg ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt
    27001 attggtatgc tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa
    27061 cagctgccgg ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat
    27121 tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt
    27181 tactgttaga cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca
    27241 ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc
    27301 tcgacacctg ctggctggcg attgctgcga cggatgatga cgcgcttaac cagcgcgtca
    27361 gtgaagccgc tgaagctcgt cgcatcttct gtaacgtggt cgatgcgccg aaagccgcca
    27421 gctttattat gccgtcaatt attgaccgct caccgctaat ggtcgcggtc tcctctggcg
    27481 gcacctctcc ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgctgc
    27541 atctgggcca ggtagcgaaa tacgccgggc aattacgcgg gcgagtgaaa caacagttcg
    27601 ccacgatggg tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc
    27661 agtcgctggc aaacaacgat cagaaagcca ttactgaaac caccgaacag ttaatcaacg
    27721 aaccgctcga ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc
    27781 tgctgacact gaaaggtctg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc
    27841 tggtttctga cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca
    27901 aacgcgcggg ataccactgc gtaccgcagg aagagataaa ccagatcctg ctacgggaag
    27961 cgcaaaaagg caaacgcgtg gtgcggctga aaggcggcga tccgtttatt tttggccgtg
    28021 gtggcgaaga gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta
    28081 ttaccgcagc ttctggttgc tctgcctatt cgggtattcc gctcacgcat cgcgattatg
    28141 cccagagcgt acgcttaatt accggacact taaaaaccgg tggcgaactg gactgggaaa
    28201 acctggcggc agaaaaacag acgctggtgt tctatatggg gttgaatcag gccgcgacta
    28261 ttcagcaaaa gctgattgaa cacggtatgc ctggcgaaat gccggtggca attgtcgaaa
    28321 acggaacagc agtcacgcag cgcgtgattg acggtacgct cacacaactg ggcgaactgg
    28381 cgcagcaaat gaacagtcca tcgctaatta ttattggtca ggttgttggc ctgcgcgata
    28441 aattgaactg gttctccaac cattaattta accaaggcca gagaattctg gcctttttcg
    28501 tttacaactc tctttatata ttcagcagat aaaaacactc cccttaataa atattatctg
    28561 ctaatgggtt atattcaacg ttacaacgac ttacaaaatt aagtcattca ctcactgacg
    28621 ccgattaaat attcctgtat cgttcttaat gagcagtctg cttatattca tataataaat
    28681 gaatattaat caacatataa tacataggga atgtaaatga acaaatttat taaagttgca
    28741 ctggtaggtg cggtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat
    28801 aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc
    28861 ggttgcggtc ctactgcaca gtaagtctct gactgaaagc ggctaacacc ggtttaattg
    28921 cctgacgccc tcggggttac ccgagggcgt atttatttct gcattctttt cgaaaacatc
    28981 atgcacaaga ttaaatactg gcaatactct cacgcaaaat ccgcagcacc gtctcttcac
    29041 cgctgcgatt tgggttaata cgaatcgcgt aatgttcgag ttgcggattc gcctggcgaa
    29101 acgttccgga aagtcgataa aaaagtggcg gaatttcata tttcgactct gcaccaaccg
    29161 ggtaaggcaa ggcaccgcgc ttttgcgcct cttccagtac tctggcggca atcggctgat
    29221 gaaactccac aatcaacacc ttcgactgcg cattggcaat caccgcgctt ttcacttccg
    29281 ccactgcgcc accgttaagt aaagccagca accgttcaga tacccccgcc tgcaccgcgt
    29341 gcatcactgg cgcgagcacc agaccacgca acacctctaa tgcctgcgcc ccctgaatct
    29401 gactgccgcc ggaatagagc gtggcgcgaa tccggctaat aacatcggca tcgcctacca
    29461 ccgcaccaac gccctctggc ccaaatagct tgaagcagga aaatgtcgac acattcgccc
    29521 cgcattcgca gccaattcgc gacaccttca tcaccgcata gttgtcatcg gttaacgctg
    29581 gaacacctgc cgcgcgcaac gttgccagca cgtctgccag cacatagcgg tcctgcggct
    29641 gctggcgcgt atgctgcacc agcgcggcat caggttgttg ctcgtcaaca acctgcttca
    29701 gtgccgacag atcattgaaa tcagccgtaa tgagcgttag ccccatctgc tcaataatga
    29761 cgcgcgtcgt tggataaacc ggcgcatcat gcaccagaag acgttgcccc ggtttgagca
    29821 aagccgccag tgccgcacga atcgcgccag tacccgcgcc ctgtaccagt gccgccgcgg
    29881 gtgcatgaaa cgcgtcagcc agcacctgtt ccacacgctg ggtaatgcgg ggctgattta
    29941 gccccggcgt taagcccaaa tcaccgcagg taagaaactc gctgccagga aaatgacggc
    30001 aaatcgtgtc caccagcgca aactgctttt gctgcgcctc aataagcgtc aggctttgca
    30061 gaggaaacgt cttcataggt gccttcctta agccggaacg aacaggccaa gccagtaaag
    30121 aacattcagc aggatgccgg tgatcattac tgctacgacc ggtgccgcca ttttctgtac
    30181 cggacggccc agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata
    30241 gcccgccatt ttgattgctg caaagatcga accgaccagc aacgccactt ccatcagcat
    30301 attcatggcg ttacggatgt tatccgacgc attacgtacc gacgggtagc gccccaacca
    30361 tttgccgatc gaacgaagca gcaagacttc cgccgaaata accactgcgc ctaataccgc
    30421 tgcaaccatc ggattcggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac
    30481 tgcatacaca ccagtggcta acgcggtggt ggcaatcaac ggtacaaaac ccagcccgcg
    30541 cataaattcc gccagagctg cctggttgat tagtgtttgc gattgttccg gcgttacgcc
    30601 tgcggaatat gctttctcca gtgtgaagat cgacacttca ctgccagcaa aaatcttcat
    30661 gctggcaacg gcggcaatca gcgccccgac aatagcgata tacggtaagt ttttgataat
    30721 ccgtgacgtg cgttcttcaa acaccgaaag cccgctggca tcaatgtcat tttcatcacg
    30781 atgacgcagg tcgtgagtaa tcgcgatccc cagcagcatc accatgccaa taaagatttc
    30841 gatggattcc ggattgagat gcgggaaata gcgcactact accacacggg tcatcagtac
    30901 caccacggcg gcgatcagac tttgcttcca gccaaactgg tagaaaatcg ccaccaacgg
    30961 gaacagtgca aaagctgaaa ccaccggcga gcttaattcc cccaggctac ctaatacatc
    31021 caccggtagc gcggtcagca gctggtttac cggtaacagg caagtaagga tcaacacgcc
    31081 ccagatagcg ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc
    31141 ggtaggaaga aagagtaacc atgcgttgag cagtccggtt ttcagggtaa aagagatccc
    31201 caccgaagcc acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacggtt
    31261 catatagcct tcaatcagtt gcggcaggat cgggcggata ccgtcatgaa aaacggccgc
    31321 cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac gccaccacga taatctgaat
    31381 atacagatcc atagtcgtgc cttttatttc agatggttaa tcagcatcgg aatcgcgtgt
    31441 tcgacgtgct caacggaaag cccaaacgcc actttccctt cggcgatcat tttggcgata
    31501 tgttcgtctt tcgctttaat gcctggtttg gcaatggtgc aacttttgtt atagccaatc
    31561 accgcgatgg caatcgacaa tgccgcgccc gcaccggtat tacacgcgcc gatgtagtaa
    31621 tccagttgcc cggatttgac tttcattgcc gcttccatgt cgttgtgaat gaaaacttca
    31681 aaacagccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca
    31741 acaccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggag tttcacgtaa
    31801 catcacatcc acatcggcct gactgaatcc tgactggcgc aattgcggaa taaaggtggt
    31861 caataaatag tcatagccat aaccaccgtt ggcttttaaa tgggagcggc gcgtaatatc
    31921 catcgacagc atgacgcggt taagcaaacc tcggtcacgt agcgcgtgga gcatcgcaat
    31981 gcgcttttcg tccgggtagt aactgttctt accgatggtg tcgaactgca catacgcgcc
    32041 gagatcgatc atcttcacaa tgttgtcgag gttgtctttc agatcgcagt gaccaacggt
    32101 gacgcgcgaa agatcaactc cgtgggcttg cagtagcgcc agttgttcca gccccatggt
    32161 gctaaaagag gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca gcgcggcggc
    32221 aataaatacc ttctcttcca gcggcgtgat ttttccttcg ctggagccta tctccgcgat
    32281 gatcccggct ttcagctccg tgccatcgat accctgttca atttcatcga ccatctcttg
    32341 cgccagttcc tgcacgctgc gggtcgccac atgttccggg aaaaacgcgt cctggtaata
    32401 accggtacag gccaccacgt tgatcccggt ttcgcgcatt acatcgagca taaattgcgc
    32461 attgcgcccc atgtaacggt tggtcatctc aatcacatta cgcacgccct gggccatcag
    32521 gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt
    32581 aaagccggag agatcaatat gcagatgctc atgggcgagg gtgtagcccg tcggatcaaa
    32641 actcataggg tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc attttgtggc
    32701 ggtggcgcgc ggaaaaattc acatacggta gcccccacat cagaaagcgt ggtgagcaca
    32761 ccaagctgcg tagcgaccat cccttgctga taaaccagca ctggcaccac ttcgcgggta
    32821 tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac caggcaatca
    32881 tctggctgca tcgcctcaat aagccgggca aggttacggt caacgacctg caaacgttcc
    32941 gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attggtacaa
    33001 ataaacgccg tcggatgggt gttaaattcg ttgaaggtga tatccataat ccgctggcta
    33061 tccaccaggt tttgccagct cacgccataa ggattactgg caatatccgc cactttcccc
    33121 accagcacgg ttggtacgcc cacttcatac agtttttgtg gcacctgcac tttttcatcg
    33181 acgccgtagc ccatatgcac gacctggaag ccgttgtcat aagcgccaga acgcggcgca
    33241 ttgatgccaa taaagcgccc ttctttgctt tctgcggcat cgaggatgcg ctggctgtcg
    33301 gttaacaggc caccaaatgt aatgacccga ccgacctgta cctgctcacg cacgatacga
    33361 ccaattttga ttgcgtcgtc aaaagagatc acagagagat tggcggtgat gttatagacc
    33421 tgacctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac ccacagaaat
    33481 tgcagatcat cgccacggcg ctccacctgc cagtcagcgt taagtaatgc ttgctcaaca
    33541 cggtcaatca catcgcgaaa gggcatccgt agcggcggta acgggcgcgt gcctaaaatt
    33601 tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt
    33661 gcggagtctg acggctgcat atctccaggc gcataaccta atgcgttgat tagccccatt
    33721 ttctccagca ttggtagctg caaatgcggc aactggctca ggatgtgacc acaggtattt
    33781 gccccggcat cctgcggacg caccaacgtg acatctttca ttgcccctac gccaaagcta
    33841 tcaatcacta acaccacaaa tcgcgccatt acgcccccag agagtttcca agactgtcat
    33901 aacgaccgac gatttctggt tcgccacggt gaataccgga cagcaacacc acatcgctac
    33961 gggtgacaaa aatctgcgtg cgaaaacaga gcactactgc actgcttacc ggaaactcgc
    34021 ctgccagcgg cagggtgtag tcgatactgc tgtcatccac agttttgaga ttggtttcag
    34081 taatcttttg attttctggc gtaaacacca gcgcatgttg cgcgtgacca cgacggtagt
    34141 aaccgccgcc gtagcagtag ctgtcgccac gaaaatgatg ggagatttca cttaaccaga
    34201 gcatcgcgat acgttcaggc tgatcgccct gctggtttgc cggaatcgtc cccgtcagcg
    34261 catgaccggg ttcggcatga gtcacaccgt attccgccag caatggcagc gaagtgcagc
    34321 tggtcgcaga aggtgcgttc agttgctcaa tcgcaatacc agatttcgcc agttgatccc
    34381 gtgcctgtat cagcgtgtga agattcggtg tcggcaaaac tttcccggca gcctcatccc
    34441 aaagcaggca agggaaatgg gttagtccgg ctaaatgcag ccccggcagt ttcttaatat
    34501 cagcaaccac ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc ggataaagaa
    34561 aatcatcgtc gctataaact ttgagcagca cagactgcac acgtccggcc ttcaccgctg
    34621 ctgcagaaac ttcccgcgct ttatcgagag taaacacggt gatgacgtca gttccctgtg
    34681 caacggcgtc agaaacctgg tgacaaggga tttgcaccag atgcccctga tgcgccaacg
    34741 gcagaccggc gcggcgcatg acccgcgcct ctttgtaatc caccgccaca atgccgctgt
    34801 agcccagtgc cagaagtttt tccgccagcc acggattacg gccaaactgc ttggtcatca
    34861 gatacagttc aatgccgtaa agtcgcgccg tctcaatcag ccgcttaccg ttttccagta
    34921 tctgatccac atcgatcacc cagctgtccg gggcgatctt gccctgctgc cacaaactta
    34981 gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc ttctacaaac atcttgttgc
    35041 ctcgtttctg ttccttgaat taaatattca tttttctgaa tatttagatt atgcgacgtg
    35101 gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc cataatccat aaagattagc
    35161 cagcaaatag ccttcttcac acggcttaac ttccagtgcg aattccttca acaacacctg
    35221 atgcaattgc acaacggctt gccagtggct ggattgcgcc agctctgcca gcaattcgtc
    35281 atccagcggc tctatttctt caccacggcg actgcgcatc agtgcgctcg ccatatgtgt
    35341 catcgccatc attccttgct cgctgcgcac cggtagatga cactctgttt ccagtacgtt
    35401 gacgacctgc atcatgcctt tgcagatgtc cttatcaata acgcctgcct cacaaagcag
    35461 gttgagtctg gtttccattt cagatgcctt tcgtttaata actcggttcc tgttccccac
    35521 tgaccactcg ttgttgatgg gcaagcaggg cctgtttatc aacaactgca cgcagcaatt
    35581 gttgcattgg gtaatcatcg actcgcgtca aaactacggc ttcggtggcc tgtaaaaatc
    35641 gcggatcgtc tgtgagcggc gtcgcttcta atcccagcat ggtcagttcg ttttccgcca
    35701 ccacgttcca gatcaccgca tcaacatcgc ctttaacaat gcgttgtaaa ctctcgtgat
    35761 aagagagatc gactcgttcc acatcactat cgccaaaaaa gacatcggtc atgattttct
    35821 gatccgccga acggttatcc agccccacgc gattcacgtt tgcggactcg cctttacggc
    35881 aaatcaactg gtgctcgcca acgtaggtgt gcggccccaa ctccagcgcg aggcataacc
    35941 ctttttgcgt gagataactt tccgccgcca gtcgggaaac aaccgccatg tcatacacgc
    36001 cgttaagtaa acactccacg cgaatatccg cgccgcgcat gtgcgcatag taaaaaggaa
    36061 taccatcaaa ctgagctttc aatccgctcg ccaggccttc gtacaaacgg gtatagggca
    36121 agggcatcgc gcacaccaca ttgttgatat ccacatgact cagcaatgct ttgttatcca
    36181 tttccaccag ataactgcca ttacgcccac ggcgttctat ccgtatcgct ccgcttgatt
    36241 ccagcgtttt caacgcggcc tgcgtcaggc caacggatga acggcattca gttgccagtt
    36301 catctatggt tttcagtcga ttaccgcact tttcacccaa caaataacgg gccagtgtcg
    36361 tgatgacgac gccttctttt ttgataaacg ttcgacgcat aataaatttt cagtaaattg
    36421 aatatttata tcttcaggaa tttgaagata aggcgcaaca gcgagatgtg gaaacggcga
    36481 ggcacttcac attttttcgg attattgcaa atgaggataa aaaaaccggg tttccccggt
    36541 ttcagagtga tgataaaagc aaaattgcct gatgcgctac gcttatcagg cctacatttc
    36601 cttgcaatat gtgcattact ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    36661 acaaagcgca atttgccagc aatagtgaat tacggcttcg ccacaaaacc aatcgcttcg
    36721 tacaccgctt tcagcgtacg agaagcgtgt gcactggctt tttccgcgcc atctttcatc
    36781 acctgttgca ggaaggcttc gtcattgcgg aaacggtgat agcgttcctg caattcagtc
    36841 agcataccgg aaacggcatc agccacttcg cctttcagat gaccatacat cttgccttcg
    36901 aactgttttt ccagttccgg gatgctctgg cccgttaccg cagaaaggat atccagcagg
    36961 ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca
    37021 gtgaccgcac gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg
    37081 ttattgcggt tatcgtcaga cttggacatc tttttggtcg gctccagcag cgacattacg
    37141 cgcgcgccag atttcggaat aaacggctcc ggcactttaa agatctcgcc atacagcgcg
    37201 ttgaaacgct gggcgatatc gcggctcagt tccaggtgct gtttctggtc ttcaccgacc
    37261 ggtaccagat tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagtcaaac
    37321 agaccagcat tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcgg
    37381 ctcagttcgc cgaagtaggt atagcagttc agtgcccagc ctaactgtgc atgttccggc
    37441 acgtgggact gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag
    37501 gccagcgtat ccagcgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg
    37561 tgttggtcaa cgatacagta aatgcaatga tagtcatcct gcatgtttac ccactgacgc
    37621 agcgcaccca tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg
    37681 atgggcttag tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag
    37741 aaggtcattt atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta
    37801 gttatatccg taggttaagc caactgatgg gcaacctgcc gcttttgctg cctgaatatc
    37861 attgcgtgag tcgccaacaa acagcatctg ttgtggggca attcccatcc gctcagccac
    37921 cagtaacagc gggtccggat gcggtttttt gttttgcaca tcatcaccgc cgataaccac
    37981 gctgaagtat ttggcgatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt
    38041 gttggtgacc aggcctagcg gcaggccttt agcctgcaat gcgcccagcg tatcggcaac
    38101 gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt
    38161 acgcagaata cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttacccat
    38221 tgttttacgc agagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaat
    38281 atctgcgccg ttaccaatcc aggtaataac gcgttcttca cctgcgacag gcaactccag
    38341 cgcatacagc gccatatcta ccgcagcagc aagaccgggt gcactgtcaa ccagcgtacc
    38401 gtcgagatca aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca
    38461 gttcactgcg catttcatca atgacttttt tgtagtctgg ctggtcgaag attgccgaac
    38521 cagcaacaaa catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc
    38581 caccgtccac ttccaatcga atgtcaaagc cagactcgtc gatacgacga cgtacttcgc
    38641 gcagtttatc cagtgtttga ggaatgaaag actgaccgcc gaaaccagga ttgacggaca
    38701 tcagcaggat cacatccagc ttatccatca cgtaatccag ataactcaga ggtgtcgccg
    38761 ggttaaatac cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa
    38821 catgctcgga ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag
    38881 gcacaatgcg atcgacgggt ttcaccatca ggtgtacgtc gataggggcg gtaatgccgt
    38941 agttacgcaa ggatttcagc accattggcc caatagtcag attgggaaca tagtggttat
    39001 ccatgacgtc aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac
    39061 ccaggcgggc aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct
    39121 tctccttgag aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag
    39181 ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac
    39241 atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag
    39301 cactggaata tggcgatcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc
    39361 aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggcgcata
    39421 aggcggatcg caatagacga cggatgcatc atctgcgcgc gccatgctat cggcgtaaga
    39481 ctcacagtag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg
    39541 gaaatagggt tttttgtagc ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg
    39601 acacagaccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg
    39661 atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc
    39721 gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt
    39781 atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt
    39841 gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg
    39901 ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt
    39961 tttcttcatg ctgactaact aattacacct tctccggctg tggagaaagc tccgacagca
    40021 tccagcgctt caggcaactc ccgcgatgat aaaccattgc gggagataac cttgattact
    40081 tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tggacatcgg
    40141 ctggcaatgt agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca
    40201 ggacatacca cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct
    40261 ctttcttcgc ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt
    40321 aatggctgga cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg
    40381 ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct
    40441 cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt gcttgccaca ggagcagctg
    40501 gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg
    40561 gttttttcgg ttcaatcacc gcctgctggc gagctggacg cgtagtggcc ggacgttcag
    40621 cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt gcgaacaggc gcgaccgtcg
    40681 cgggttcagt cggcaacgtg gaattgaccg ccacattatt caactgttgc tgattttgtg
    40741 gctgggtcag cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg
    40801 ccgccggggt ttgcccttga gtcggcgtag aagagatcgg cggcagagaa acatcctgct
    40861 gagtattttc cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg
    40921 cattgccagc aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagaggtcg
    40981 agggggcttt tagcgcagaa ccgataccga tgatcaacag caggagaacc agaatgccga
    41041 cgcccatcat catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgtg
    41101 gacgacgctc tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt
    41161 cgagttccgg ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt
    41221 catcaagttc aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac
    41281 gttcagaaga ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt
    41341 cttctggttt gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc
    41401 aattaacctg aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct
    41461 gattgacaat cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca
    41521 ctcttaccaa ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca
    41581 cgcagcatat gcggtaaata cgcctgcgca gacatttcgc gcggcccatt gaccggtaac
    41641 ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga
    41701 cgttccgacg tccgcgccgc catcaccata cccgcagcaa ccgcttcacc gtgtaaccaa
    41761 ttgccatacc ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa
    41821 gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa
    41881 cggcgaatac agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct
    41941 tccagccagt tgaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc
    42001 cccgacgcta actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa
    42061 gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg
    42121 ccgccaacgg aggaatcgac ctgcgacagc aatgtcgtcg ggacttgaat gaaacgtaca
    42181 ccgcgctgat aactcgccgc cgcaaagccg gtcagatcgc ccactacgcc gccgccaagc
    42241 gccaccagcg tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg
    42301 agtacagcca ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca
    42361 cccgcctgtt caagtacgcc gcggacctta tcgagataca ggggagccag ggtttcgttg
    42421 gtaaccaaca tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac
    42481 aaaccagatg cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc
    42541 ataacgcgac atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt
    42601 agttgctttc cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag
    42661 tacgaatggt cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt
    42721 ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg
    42781 tgcgtgcaag ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa
    42841 gacggttacg cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct
    42901 gtttctcggt caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt
    42961 ctaaatcgaa aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat
    43021 cgtaaaattc catattgagt tgttgagcta actggcgccc aatagtgctt tttccggcac
    43081 ccataggccc aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac
    43141 tattcgttaa tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct
    43201 cataagatat tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac
    43261 tgaataaatc acccatccct ctgcatatct ggtcgctgca agcgcgctgc cttgctacca
    43321 ccgctctggc gattaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct
    43381 gaatgataaa tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta
    43441 ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcgtaaaaac
    43501 attaccgctt ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact
    43561 aactcgcgtc gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg
    43621 tcgccaagca acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc
    43681 agcgccaacg tttctccgct tttgacctcg acctgcgttt cgatctcctg cttatcaatc
    43741 gccaacactt cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt
    43801 aatttcagcc ggatgcgacc tttttgtaac accgtgggcg taacttccat ccccaggacg
    43861 gcctctttaa attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt
    43921 tcgctcccct gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg
    43981 ataatatcca gctgctgttt ttgttcgagc gcggaaagct caagatccag taaacgtccg
    44041 ttgatgcgtc caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgctg
    44101 ccaagcgtgg tgacttgccc aacgccacca gcttgctgcg catcagccaa cgtccatttc
    44161 acgcctaact cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc
    44221 tgcccgaccg gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg
    44281 ttatctcgca gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc
    44341 agtagcttct ccccggcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg
    44401 cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg acgggcgata
    44461 ttgtcattct gccaggcaac ggaatgcacc gagagaatgt tgccctcctg gcgcgttatc
    44521 aatccggcgc ttttcactac agtttgtagt gcctgcttcc agggaacgtc agttaaatgt
    44581 aacgacaccg taccgctgac gtctggcgaa accaccaggt tcaacttctc ctgttcagcc
    44641 agcgcctgca acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc
    44701 tttgccgcct gtacgccggg tataagcatc aacagtagtg cggctatcca ttgcttcatt
    44761 tgtatctcct tgccgttgcc acaaccagtg tggcggttcg cagtttgtcc cggttcccag
    44821 cgttagtgcg tctggcgtca gctgtaaaat tgtccagccg ttttccagca cgtcgttttg
    44881 ctgcacccgt cgccattttt tttgcccgtc ttttattaca ccgatgatgc gctcacctcg
    44941 ccctaccatc ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg
    45001 cggtttaaaa gggtcacgca taccggttaa aaggcacaat gtaatacctg ccaacaacca
    45061 gcgtttaacc ctcatgcggc gtctccagtt gtagcgtgaa caaaaggtca tcaccttcca
    45121 cgcttaacga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    45181 gcactgcttc ccacagcgtt ttcaacgcca actcacctcc ctgcgcggat ggatgccagt
    45241 aaaccagttg cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct
    45301 cgctgaaggg agtggtgtca accaggcgat acagtgcggc ccactgctgg tgatgacttg
    45361 cccgtagtcg aattaatgcg tcgctctctt catggtgtgt cgatgacaga aaaatgagcg
    45421 taactaacat cagcaaccag actgcccagc aaagctggcg gaggcggggt gatgtggcga
    45481 accaccagtc aaagagcatg tccatcgcta accttccttg ttaactgata ctcaaattgc
    45541 cagcgtccct gcgcatcctg ctgcgtggcc ccccgctgat tgagatgaaa agatgcatcc
    45601 tggcggagtg acgtttccag cgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc
    45661 tccagcgtac cttgctgcca gcttatcgtt gtcagccagg catgttcggg taaaagcgcc
    45721 gccagtgctt ccagcgcaga ttgccagtca cgggtaaatt gtcgctggag ctgcctttgc
    45781 aaacgctgtt cgcgtaactg ctggcgttcc agcaaacgtg gtttcgttat ctgtaagctg
    45841 cgggcgagtt gttgttccgc ctgaagcaaa acggtgtcta cgcgtgcttc ggcgttgctt
    45901 gtcagacgca gtattagcgt tatcccgaca gccagcagca gaggcgcaac gaacatcagc
    45961 aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    46021 gggttcatca gtacgccttc cctagcgcca gccccagcgc gatggcaaag tctccacctg
    46081 gcggcggtag cggcggctga cgaacagaaa ccgcctccca gggatcaaat ccgccttcgc
    46141 cacatatcgc gacgctttcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    46201 tcatccccac cgccagtttg cgcccccagc gatagcgcgt cgcccacagc cactgttcgt
    46261 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    46321 cactggcatc cggggtgatc gcgctaacat gaacacgcaa cctttctgcc agagtaagca
    46381 gcgttgccaa ctctttgctt tgcgcggcag tcacgttata ggcgggactg agtgagtctt
    46441 cgctgtaatc gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtcccgg
    46501 acagccaggc cgtttgctcc cgctcaccaa gggacatcga cggacgtgga aatgaccgct
    46561 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    46621 tccacggtaa caacgtttta gccagccgct gcacatcaac aatccgccca tctttgataa
    46681 tgtcgttctc cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gtgccccaac
    46741 cgctaccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa atgccattgt
    46801 gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg cttgccttta
    46861 tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa tttccagtga
    46921 agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga gcaggctcga
    46981 tttatggcct ataccgctac atcgagccac aactgccgga tgtggcgaca ttaaaagatg
    47041 ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt gctcaatacg
    47101 gtgagaaacg tcgtattccg gttacgttgg atcaaatccc gccggagatg gtgaaagcct
    47161 ttatcgcgac ggaagacagc cgcttctacg agcatcacgg cgttgacccg gtggggatct
    47221 tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg agtaccatta
    47281 cccagcagct ggcgagaaac ttcttcctca gtccagaacg cacgctgatg cgtaagatta
    47341 aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgag atcctcgagc
    47401 tttatctgaa caagatttac ctcggttacc gcgcctatgg tgtcggtgct gcggcacaag
    47461 tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg atagccgggc
    47521 tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc
    47581 gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccaacaa cagttcgatc
    47641 agacacgcac tgaggcgatt aacgctaact atcacgcgcc ggagattgct ttctctgcgc
    47701 cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa agtgcctatg
    47761 aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc gcacagcagg
    47821 cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg
    47881 tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat acgctgaagg
    47941 cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat cctcaggaag
    48001 cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc gttcgctggg
    48061 cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg accgatgttc
    48121 tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg ctggcgcaag
    48181 tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaacggtgcc gttatggcgc
    48241 tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag gcactgcgtc
    48301 aggtaggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa ggtctgacgc
    48361 tggcaagtat gttgaacgat gtgccaattt cccgctggga tgcaggtgcc ggttctgact
    48421 ggcagccgaa gaactcacca ccgcagtatg ccggtccaat tcgcttacgt cagggactgg
    48481 gccagtcgaa aaacgtggtg atggtacgcg caatgcgggc gatgggcgtc gactacgctg
    48541 cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg
    48601 cgctgggttc agcgtcattc accccaatgc aggtggcgcg cggctacgcg gtcatggcga
    48661 acggcggctt cctggtggac ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg
    48721 tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattccg gtgatttacg
    48781 gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc gctatctccc
    48841 gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat caggcgttag
    48901 tggcgaagac cggcgcgcag gaatacgcac cgcacgtcat caacactccg ctggcattcc
    48961 tgattaagag tgccttgaac accaatattt ttggtgaacc cggctggcag ggtactggct
    49021 ggcgagcagg tcgtgattta caacggcatg atataggcgg aaaaacgggt accactaaca
    49081 gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg gtctggattg
    49141 gctttgatga tcaccgtcgt aatctcggtc atacaacagc ttccggagcg attaaagatc
    49201 agatctcagg ttacgaaggc ggtgccaaga gtgcccaacc tgcatgggac gcttatatga
    49261 aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gccgccgggt attgtgacgg
    49321 tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc gaagagtatt
    49381 tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg accattatcg
    49441 ataatggcga ggcacaggaa ttgttctgat taaaaaggcg cttcagcgcc tttttaagtt
    49501 tgctgacaaa gtgcactttg tttataccgg atacggcgtg aacactttac ccgtctaaaa
    49561 acatctagaa aaatcaatat attgcaggag ccacacaggc ctgataagca ttatgcatca
    49621 ggcaaacttc acgcatttac aaccgcccct gccccttcaa ccattcgcgc acgaggaaaa
    49681 gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc atcatatgcg
    49741 ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc gattccgggt
    49801 agagatcttg cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga
    49861 gcttcttcaa aaaagtcaga tcgttcgcac caaatcccac ctcttctttt agctcgcggt
    49921 tcgcggcttc gaagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt
    49981 aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc acaatcggca
    50041 caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat
    50101 tgctgaactc cagatccacg ctttcgacgg taaacagtcg ggaacgggct acagtttcaa
    50161 cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc
    50221 agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg ttatttacat
    50281 ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa
    50341 ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg
    50401 atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat
    50461 gttttgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga
    50521 gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga
    50581 taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa
    50641 cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc
    50701 aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatttct ctggcgctga
    50761 atgccgaaag caacaacgtc atgatgctaa cacacgctat cacgcgttac ggcatctcta
    50821 ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccccctt
    50881 tctgggagca gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc
    50941 cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa actcgcggct
    51001 atgccttgca acctgttccg tcaacacagg cgtcgattcg cggggaagag agtgagcaaa
    51061 tcgagctact caatattcgc aaagaaacgc acgaagagta tgcgctaagt cgtccgcgcg
    51121 ggctgcgcga agcgttgctg attgtcgcct ccttcctgat gttctttttc tgcctgatta
    51181 ccccggatgt atttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc
    51241 tgtgggggct attcgcgccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg
    51301 gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt
    51361 cactcggtat tatcgacctg gtctaccccg cacactggca gccctacatt gctcatgatc
    51421 tcggccaaca aaccgatatc gatatctatc tcgaccgcca cgtagtgcgt caggggcgat
    51481 atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga
    51541 ttatcgctgc gggttcgctg ctggtgctgt ttatgctgtt attctggatc ccgctggata
    51601 tgccgctgaa attcactctc tcatggatga aaggcgcgca gaccattgaa gccaccagcg
    51661 tgaaacaact ggctgacgct ggcgtacggg ttggcgatac attgcgtatt agcggtacgg
    51721 gaatgtgtaa tattcgcact tccggtacct ggagcgcgaa aaccaactca ccttttttac
    51781 cgtttgactg ctcgcagatc atctggaatg acgcccgctc attgccatta ccagaatctg
    51841 aattggtcaa caaagcgacg gcattgaccg aagcagttaa tcgccagctg caccctaaac
    51901 cggaagatga atctcgcgtc agcgcctcat tacgttcagc aattcaaaaa tccggcatgg
    51961 tattgcttga tgattttggc gacatagtac tgaagacagc ggatctgtgt tctgccaaag
    52021 atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg
    52081 cgctggtaaa acgcgccaac gccgggaaac tcgatggcgt gaatgtgtta ttacgcccgg
    52141 tgagtgcgga atcgctggat aacctggtgg caacctccac cgcgccgttt atcacgcatg
    52201 aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca
    52261 gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactacc
    52321 cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt
    52381 ttaacgccga gggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatatag
    52441 gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc acgctgctgc
    52501 tactgacgat gctgggtagc gccatttata atggcgtaca ggcctggcgg cgttaccagc
    52561 gtcatcgtac tcgcatgatg aagattcagg cctattatga aagctgcctg aacccacaac
    52621 tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc
    52681 tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat tgcctggcag
    52741 gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac
    52801 tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt tacgccacag
    52861 gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac
    52921 tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg
    52981 ggaccgcgcg ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc
    53041 ggtaagcagc gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag
    53101 cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg
    53161 aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga
    53221 acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt
    53281 tactgcctcg gcgtgactaa tcctgactcc gggattgccg agaaacagta tcaacgccat
    53341 ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa
    53401 agaaaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa
    53461 aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg
    53521 cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga
    53581 cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc
    53641 cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg
    53701 taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg
    53761 cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat
    53821 tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg ttcgcggcga
    53881 actggtaacc gtttcggaaa ccctgcaaca gatccttgag aaccacgatt atccgcagcc
    53941 cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa
    54001 gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc tggcggttat
    54061 taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga
    54121 aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca tcaccccgag
    54181 cgaaggtgaa cgctatcagg gcgtggtggg tctggaaggt gatactctgg cggcctgcct
    54241 ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga
    54301 cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg cgcaaaatgc
    54361 ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact
    54421 gttgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt
    54481 ttatgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgacgc
    54541 gctaaaaacg ctgccggatg aagaagtcga tagcatcctg gcggaagatg gcgaaattga
    54601 catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata ttgctgaaat
    54661 ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag
    54721 ggctctgccg gattttacga cgtaatggcc tgatgcgctt atcagaccta cggattcaat
    54781 gcaatttatc gcaggtaaga taatgtgccg aatccaataa aaatcagata tgcgctgtca
    54841 ttcattcaga ttcaaatcca gtctttcgcc atccaccgta agatcgcgcc acactttagg
    54901 cttcgtcccc agttttccat cctccgcagc catgagtaat ttttctttcg ttattacagc
    54961 cgggagtgaa ttcatcgtaa tctctttcca ttctttccca gcaggatcaa taccatagat
    55021 aatgtatctt tcatcattaa aggcaaacag gatccgctct gccctgccat cactattcag
    55081 atcttgctct accagcacgc aggcatcttt ttcaatgcat atcattgcgt tatagcgatt
    55141 tttcatcact gccgaccaga atatcgtatc cggttttcca gaacctgagg caataaatac
    55201 attatctgct aatgcttttt ccgatatctg ctgctgaaga cgttgtactc catcaaatgc
    55261 catcagcaga ccccgcctgc gtttcgggtc tttcatatac tcggcatcgc ttttcagcga
    55321 ctcaagcgcc gcacgtccat agcgaccgct ctgttcgagc atatagagac ttacctggtc
    55381 tggcgtgttt ttgccgctct gataacgcgc catatgactg ttcacgctaa tgcgcatact
    55441 gtccagcacc ggagaattaa gaagcaccag tattaccaac accaataacg aaaccgcaag
    55501 gttcactttg ccctgaagaa caagggggtt ttgcccttta cgccagacga tactgacaaa
    55561 ataccccagc gaccatacca gtaacaccag caccgccagc gcgccctgca agcggtcgac
    55621 agtccagccg tactgagcga cccgcagcca taacgcccag gcggcgacga acacatacag
    55681 cggagcaacc agcaaagcgg ttttaatcag gcaacgcaac ggccccgtcc agggaagaga
    55741 cgctttttgc ggatcgcgga caatagccat taagatcaat tgcaaaaagg ccagcgttaa
    55801 cagcaaccca gcggcggaga tgtggcgaga aatcgcgctc aggcccgtaa acggcagggt
    55861 gataataaac atcagggtta gcaatgatac taacggcagc aaccccgtgg cgattaacgt
    55921 gaacaacttt tgaatagagt cgattaaacg tgactgtgta cgcgcgagga tcaccgccag
    55981 tgcggtaacc agacctaacg tgagataaat aaaccagtcg gttgcaaaaa agagcgtttt
    56041 gaaaaacgtg atgccaacaa gtttaaacaa ctctccccac agtaaaagta ccagccacgt
    56101 caggccattg gcgagaaaaa tcaccagtaa tataagtacg ttatgccata ctgactggta
    56161 aaaatagcga tagcggctac tgtcattgcg gatgcgaagg ctttgttgta tccacggtag
    56221 caacaacatt gccatcagca gcagatagca accaaaatcc caaagcgcct tttctgctct
    56281 ccaggggttc atgccatcaa tttgccactt tagccaaccg ctcatcccta gcgtggcaat
    56341 aaacaccagc gccagccatc cccaaaggcg cttctgttta aaggagatca cggagaagag
    56401 taaaacggat gaaaacgcga cggtcgcagg cacgccataa acaatccaac tgtgattttt
    56461 tccggcaagc caggcgatca gcaggtagca aaccagaccc tgaagtaatc cggtagcaat
    56521 catcccccag cgtgttgccg gtgaaagttc aacgttatcc atgttagtta tccagaatca
    56581 aaaagtgggt taattattgc atccgggccg tagtattttg cttttttcag aaaataatca
    56641 aaaaaagtta gcgtggtgaa tcgatacttt accgattgaa tttgcatcaa tttcattcag
    56701 gaatgcgatt ccactcacaa tattcccgcc atataaacca agatttaacc ttttaagaac
    56761 attttccaca cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg acaggaatca
    56821 cggagttttt tgtcaaatat gaatttctcc agatacgtaa atctatgagc cttgtcgcgg
    56881 ttaacacccc caaaaagact ttactattca ggcaatacat attggctaag gagcagtgaa
    56941 atgcgcgtta acaatggttt gaccccgcaa gaactcgagg cttatggtat cagtgacgta
    57001 catgatatcg tttacaaccc aagctacgac ctgctgtatc aggaagagct cgatccgagc
    57061 ctgacaggtt atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt cgataccggg
    57121 atcttcaccg gtcgttcacc aaaagataag tatatcgtcc gtgacgatac cactcgcgat
    57181 actttctggt gggcagacaa aggcaaaggt aagaacgaca acaaacctct ctctccggaa
    57241 acctggcagc atctgaaagg cctggtgacc aaacagcttt ccggcaaacg tctgttcgtt
    57301 gtcgacgcct tctgtggtgc gaacccggat actcgtcttt ccgtccgttt catcaccgaa
    57361 gtggcctggc aggcgcattt tgtcaaaaac atgtttattc gcccgagcga tgaagagctg
    57421 gcaggtttca aaccagactt tatcgttatg aacggcgcga agtgcactaa cccgcagtgg
    57481 aaagaacagg gtctgaactc cgaaaacttc gtggcgttta acctgaccga gcgcatgcag
    57541 ctgattggcg gcacctggta cggcggcgaa atgaagaaag ggatgttctc aatgatgaac
    57601 tacctgctgc cgctgaaagg tatcgcttct atgcactgct ccgccaacgt tggcgagaaa
    57661 ggcgatgttg cggtgttctt cggcctttcc ggcaccggta aaaccaccct ttccaccgac
    57721 ccgaaacgtc gcctgattgg cgatgacgag cacggctggg acgatgacgg tgtgtttaac
    57781 tttgaaggcg gctgctacgc aaaaactatc aagctgtcga aagaagcgga acctgaaatc
    57841 tacaacgcta tccgtcgtga tgcgctgctg gaaaacgtca ccgtgcgtga agatggcact
    57901 atcgactttg atgatggttc aaaaaccgag aacacccgcg tttcttatcc gatctatcac
    57961 atcgaaaaca tcgttaagcc ggtgtccaaa gcgggccacg caactaaagt tatcttcctg
    58021 accgcagacg cctttggtgt gctgccgccg gtttctcgtc tgactgccga tcaaacccag
    58081 taccacttcc tctctggctt caccgccaaa ctggccggta cagagcgcgg catcaccgaa
    58141 ccgacgccaa ccttctccgc ctgcttcggc gcggcattcc tgtcgctgca cccgacgcag
    58201 tacgcagaag tgctggtgaa acgtatgcag gcggcaggcg cgcaggcgta tctggttaac
    58261 accggctgga acggcactgg caaacgtatc tccattaaag atacccgcgc cattatcgac
    58321 gccatcctca acggttcgct ggataacgca gaaaccttca ctctgccgat gtttaacctg
    58381 gcgatcccaa ccgaactgcc gggcgtagac accaggatcc tcgatccgcg taacacctac
    58441 gcctctccgg aacagtggca ggaaaaagct gaaacgctgg cgaaactgtt tatcgacaac
    58501 ttcgataaat acaccgacac ccctgcgggt gccgcgctgg tagcggcagg tcctaagctg
    58561 taatcagctg atatctcatt gaccccttat ccaaacggat aaggggtttt tttatctaat
    58621 tagctaccaa tatgtacacg gtacacttat aattatcaat agaccgcagg atgcattatg
    58681 ttcaccttta ttgaactaca aggatttagc aaacgccgcc agttattgct acctgatgat
    58741 gagttccggg cttttcagga actactcatt caagatccca ctgccggtga cattatcgca
    58801 ggcacaggca gttttagaaa aattcgctgg aaccgttctg gaatggggaa acggggtggc
    58861 attcggataa tttattacaa cgtaacacgc aatggccgaa tttatctggc gttgatttat
    58921 cctaaaaatg aacaggatga tttaacagaa gaacaaagaa aggcattgaa actattgtcc
    58981 gagaagctgc tttaatgttg aagctccaca aattccacca tggccgaaat gtcaacggag
    59041 gcattgaaaa cagatgaaag atgaattatt tgctgatctt cttgccagcg cagaagaaat
    59101 ggtacgcatc gaaaagggtg aagaaacgcc tgaaccagag cacgtccata cttttagcga
    59161 aatcgatgta aaagcgatcc gtgaagcaac gggtttacgt cagcaagatt ttgctgtcgc
    59221 cgtaggggta agttatgacc tggtcaaaag ctgggaaacc aaacgccgtc aacctaccgg
    59281 agcaccccga aaattgctcc tgcttttgca agcgaatcct ttcattatca atcaattgaa
    59341 aacaatctaa aaacaggagg caccttcgcc tcccgtttta tttacccttc ttttgtcgtg
    59401 ccctgcgccc gcgttaccgg cactggcagc caggcgcgaa tggaaagccc gccccgctcg
    59461 ctgctgccaa gctccagcat gccgttatgg ttatccacga tacgctgcac aatcgccagc
    59521 cctaatcccg tgccgctaat ggtgcgcgca ctgtcgccgc gaacaaacgg ctggaacagg
    59581 tgcttacgtt gttccggcgc aattcccgga ccatcatctt ccacctggaa ccaggcgcga
    59641 ttcggctccg ttccgctgct gactttgatc cagccattgc catagcgggc agcgttcacc
    59701 accatattcg ccaccgcacg tttgatcgac agcgggtgca ttttcacctc aatgctgccg
    59761 gggtaaagcg cggtttcaat ttcccgctca tagccacttt cggcggcaat cacctcaccg
    59821 agcactgcgt tgagatccgc catttccatc ggcatctcct gcccggtgcg caggtagtcg
    59881 ataaactgct caatgatggc gttgcactct tcgatatctt tattgatcga ttcggccagg
    59941 tagccatcct gctcgctcat catctcggtc gccaggcgaa tgcgcgtcag cggcgtacgc
    60001 agatcatgac ttacccccgc catcagcagc gtgcgatcat ccgccagttg cttaacacca
    60061 gccgccatat ggttaaaggc gcgggtaacg gaacgcacct ccgaagcgcc atattcacgc
    60121 agcggcggcg gaataatccc tttaccgacc tgcagagcag catgttcgag atcgaccaac
    60181 ggtcggttct ggatacggat aaacagccac gccccgccta tcgccaatag cataatcgcc
    60241 agcgtatagc ggaacagcgg agaaaaatca ccctgatgaa tttcggtcag cggcacgcgc
    60301 acccagatat tgggcgacag ccaggttttc agccagacaa caggcgaact tttgttgacc
    60361 tcaacgcgca cttccgtcgg gccgcccagt tgctgcgcca tctgatggct taagaattca
    60421 tagtgttgtg cccaacgcag acctgcctct tcggcagctt cgttggaata gagagagatc
    60481 cccagctcac ggtagatctc ccgacggaaa gcgggaggca caaccaactg cgtgccgtcc
    60541 tccagttgca gtttgtcggt catcaacata cgcacttcgt acgcgaggac tttattaaac
    60601 tgctggaggc tcggcaaaat cgcgaagttc agcaccacca gataagtcgt caccaggctg
    60661 acgaacagca aggtgacgat gagcaataac gtacgggcaa atgaacttcg tggcgagaag
    60721 cgcaatcgcc tcatgcttta gaaccgtccg gtacaaagac gtagcccaga ccccagacgg
    60781 tctgaatgta acgcggatgc gccggatctt cttccaccat gcggcgtaga cgcgaaatct
    60841 gcacgtcgat ggagcgttcc attgcggagt attcacggcc acgggcaagg ttcatcagct
    60901 tatcgcggga gagcggctca cgcggatggc tgaccagtgc cttcagtacc gcaaactcac
    60961 cgctggtgag cggcatcggc tcatcttcgc ggaacatttc acgcgtaccg aggttaagtt
    61021 tgaacttacc gaaagcaatt accgcctctt cctgtgacgg cgcgcccggc agttcgttgg
    61081 cctgacgacg cagcaccgca cgaatgcggg ccagcaattc acgcgggtta aacggttttg
    61141 gaatgtagtc gtcagcgcca atctccaggc caacgatacg gtccacttct tcccctttcg
    61201 ccgtcaccat aatgatcggc atcgggttgc tctggctacg aagacgtcgg caaatcgaca
    61261 agccatcttc accaggtaac atcaaatcca gtaccataag atggaaagat tcacgagtca
    61321 gcaggcgatc catctgttct gcattagcga cgcttcgaac ctggaagcct tgttcggtga
    61381 gataacgttc cagcagcgcg cgcaggcgca tatcgtcatc gaccaccaga atcttgtagt
    61441 tctcttgcat tgtttgtact cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa
    61501 aaagctcacg ttcgtcacca gctaaatctg gtatgaattt cagcctaaat tgttacaaag
    61561 catattaaac agcagcttaa gtatacaatt tattcggcga aacattattg attctgttga
    61621 tatgatcacg ttatacccaa tgtgcgcatt atcaaacaga caaagggaat caacgagatg
    61681 aaaacgcccc tggttacccg ggaagggtat gaaaaactca aacaagagct taattatctc
    61741 tggcgtgaag aacgcccgga ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac
    61801 cgcagcgaaa atgctgacta tcagtataat aaaaagcgcc tgcgtgaaat cgaccgtcgc
    61861 gtgcgctatc tcactaaatg catggaaaat ctcaaaatcg tcgattactc ccctcagcag
    61921 gaaggcaaag tcttttttgg tgcgtgggtg gagattgaaa acgacgatgg cgtgactcac
    61981 cgtttccgta ttgtcggcta cgatgaaatt tttggccgta aagattacat ctctatcgat
    62041 tccccgatgg ctcgcgcatt gttgaaaaaa gaggtgggcg atttggcggt ggtgaatacc
    62101 cctgccgggg aagcgagctg gtatgtgaat gctatcgagt acgtgaaacc gtaaggaaga
    62161 gtcttaacct cctgccgatg gctggcattt ttgccagcca gtccgtataa ctatcccctg
    62221 atttttgatc cgaaaagatg aactcaaacc atgatgaatg attcgttctg ccgcattatt
    62281 gcgggtgaaa ttcaggcgcg cccggaacag gttgacgctg ccgttcgcct gcttgacgaa
    62341 gggaataccg tgccgtttat cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac
    62401 acgcagctgc gtaatctgga aacgcgtctg agctatctgc gcgagctgga agagagacgt
    62461 caggcgatcc tcaagtccat ttccgagcaa ggcaaactca ctgacgagct ggcgaatgcc
    62521 atcaacgcca cactaagcaa aaccgaactc gaagacctct acctgcccta caaacctaaa
    62581 cgccgcaccc gcgggcaaat cgccattgaa gcagggcttg agccgttggc tgacctgctg
    62641 tggagcgatc cgtcacacac gccagaagtc gccgctgcac aatatattga tgccgataaa
    62701 ggcgtggcag ataccaaagc cgcgctggac ggcgcgcgct atatcctaat ggaacggttt
    62761 gccgaagatg ccgcgctgct ggcgaaagtg cgtgattatc tgtggaagaa cgcgcatttg
    62821 gtctcaaccg tggtgaatgg taaagaagag gaaggggcga aattccgcga ctatttcgat
    62881 catcacgaac cgctgtccac ggtgccttct caccgcgcgc tggcgatgtt ccgtggtcgt
    62941 aacgaaggcg tacttcagct ttcgctgaat gccgatccgc agttcgaaga gccgccgaaa
    63001 gagagctatt gcgagcaaat catcatggat caccttggcc tgcgcctgaa caatgccccg
    63061 gcggatagct ggcgcaaagg tgtggtgagc tggacgtggc gcatcaaggt gttgatgcat
    63121 ctggaaactg aactgatggg caccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc
    63181 tttgcccgta acctgcacga tctgctgatg gcggcccctg ccggactgcg tgcaacgatg
    63241 ggcctcgatc cgggtctgcg taccggggta aaagtggcgg tagtcgatgc gacaggtaaa
    63301 ctggtggcga ccgataccat ttatccgcat accgggcagg ccgcaaaagc agcgatgacc
    63361 gttgctgcgc tgtgtgaaaa gcataacgtt gaactggtgg cgatcggcaa cggtacagct
    63421 tctcgcgaaa ccgaacgttt ctatctcgac gtgcagaagc agttcccgaa agtgaccgcg
    63481 cagaaagtga tcgtcagcga agctggcgcg tcggtttact cggcttccga gctggcagca
    63541 caggaattcc cggatctcga cgtttcgctg cgtggcgcgg tgtctatcgc ccgtcgtttg
    63601 caggatccgc tggcggagct ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat
    63661 cagcatgacg tcagccagac gcagctggca cgcaaactgg atgcagtagt agaagactgc
    63721 gtaaacgccg ttggcgtcga tctcaacacc gcttctgttc ctctgttaac ccgcgtggcg
    63781 ggcctgacgc gcatgatggc gcaaaacatc gtggcctggc gcgatgagaa cggccagttc
    63841 cagaaccgtc agcaactgtt aaaagtgagc cgtctggggc cgaaagcctt cgagcagtgc
    63901 gcgggcttct tgcgcattaa ccatggtgat aacccgctgg acgcgtctac cgtccacccg
    63961 gaagcctatc cggtggtgga acgcattctg gcagcaacac agcaggcact gaaagatctg
    64021 atgggtaaca gcagcgagct gcgtaacctg aaagcgtctg actttactga tgaaaaattc
    64081 ggtgtgccga cggtaaccga catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt
    64141 ccggaattta aaaccgctca gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg
    64201 ggtatgatcc tcgaaggtgc ggtgaccaac gtcaccaact ttggcgcgtt tgtcgatatt
    64261 ggcgtgcatc aggacggcct ggttcacatc tcttcattgt cgaacaagtt tgtggaagat
    64321 ccgcataccg tggtgaaagc gggcgacatt gtgaaggtga aagtgctgga agtggatctt
    64381 cagcgtaaac gtatcgccct gaccatgcgt ctggacgagc agcctggcga aaccaacgcc
    64441 cgtcgcggtg gcggtaacga tcgcccgcaa aacaaccgcc cggcagccaa accgcgtggg
    64501 cgtgaagcgc agcctgccgg taatagcgcg atgatggatg cgctggcggc ggcaatgggc
    64561 aaaaaacgtt aaacgcccgg actggcccct aaacctgcgc gattaggcgt tcacgccaca
    64621 tctggcaacg aacgccttgc ctgacataaa agtgccggag aatatctccg gcctttttat
    64681 tccacaagca aactcataat atatcccatt aatatttatc acttcattaa caactgaaac
    64741 cttaattaaa cattaaccag ccctaataat tcgtcatgcg aattatattt tcgctgcgat
    64801 ataaccttga gccatatcaa cattgagtca gattattatt caaaccaaca ttcacacaca
    64861 ttttaagtat tgctgataga aaccattctc attatcattg tactgttgat tatttaatct
    64921 ctccttcgtt ggcaaatcat ctggtctcat gtcgctgtca aacgccccat gaggtagtta
    64981 tccagttaat gagaaacaag taggcaccta tgcaatacac tccagatact gcgtggaaaa
    65041 ttactggctt ttcccgtgaa atcagcccgg catatcgcca aaaactgctt tctcttggca
    65101 tgttacctgg ctcctctttt aatgtggtgc gcgtcgctcc actcggcgac cccattcata
    65161 tcgaaacccg tcgtgtgagc ctggtattac gaaaaaaaga tctggcctta ttagaagtgg
    65221 aagcggtttc ctgttaatac ggtgataaca acaatgaaaa aattaaccat tggcttaatt
    65281 ggtaatccaa attctggcaa gacaacgtta tttaaccagc tcactggctc acgtcagcgt
    65341 gtaggtaact gggctggcgt taccgtcgaa cgtaaagaag ggcaattctc caccaccgat
    65401 catcaggtca cgctggtgga cctgcccggc acctattctc tgaccaccat ctcatcgcag
    65461 acctcgctcg atgagcaaat cgcctgtcac tacattttga gtggcgacgc agacctgctg
    65521 attaacgtgg tggatgcgtc taaccttgag cgtaacctgt acctgacgct gcaactgttg
    65581 gaactcggca ttccctgcat cgtggcactg aacatgctcg acattgccga gaagcaaaat
    65641 attcgtattg aaattgatgc tctgtcggcg cgtcttggct gcccggtgat cccgctggtt
    65701 tcaacccgtg gtcgcggtat tgaagcgctc aagctggcga ttgatcgcta taaagctaac
    65761 gagaatgtgg aactggtgca ttacgcacag ccgctactca acgaagcaga ttcactggca
    65821 aaagtgatgc cttccgacat cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg
    65881 gaaggcgata tctacagccg cgcctacgcc ggtgaagcgt cgcagcatct ggatgccgcc
    65941 ctcgcccgtc tgcgtaatga gatggacgat ccggcgctgc acattgccga tgcgcgttac
    66001 cagtgcattg ctgccatctg tgatgtggta agcaacaccc tgacggcaga acccagccgt
    66061 ttcaccactg cggtagataa aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc
    66121 tttgtgatgt acctgatgtt cctgctggct atcaacatcg gcggggcgtt acagccgctg
    66181 tttgatgtcg gttccgtggc gctgtttgtg catggtattc agtggattgg ctacacgctc
    66241 cacttcccgg actggctgac tatcttcctc gcccagggcc tgggtggcgg tattaacacc
    66301 gtgctgccac tggtgccgca gatcggcatg atgtacctgt tcctctcctt ccttgaggac
    66361 tccggctata tggcgcgtgc ggcgtttgtg atggaccgcc tgatgcaggc gctgggcttg
    66421 ccggggaaat cctttgtacc gctgatcgtc ggcttcggtt gtaacgtacc atcggtaatg
    66481 ggcgcacgta cgcttgatgc accgcgtgaa cgcctgatga ccatcatgat ggcaccgttt
    66541 atgtcctgcg gcgcacgtct ggctatcttc gcagtattcg cggctgcctt cttcgggcag
    66601 aacggtgcgc tggcggtctt ctcgctgtat atgctgggta tcgtaatggc ggtgctgacg
    66661 ggcctgatgc tcaagtacac catcatgcgc ggtgaagcga cgccgtttgt catggagctg
    66721 ccggtctacc atgtaccaca cgttaaaagc ctgattatcc agacctggca gcgtctgaaa
    66781 ggcttcgttc tgcgtgctgg taaagtgatt atcatcgtca gcattttcct gagcgccttc
    66841 aacagcttct cgctgagcgg aaaaatcgtc gataacatca acgactcggc gctggcttcc
    66901 gtcagccggg tgatcacccc agtcttcaag ccgattggcg tgcatgaaga taactggcag
    66961 gcaacggttg gcctgtttac aggcgcaatg gcgaaagaag tggtggtggg cacgctcaac
    67021 accctctaca ccgcagaaaa tattcaggac gaagagttca atccggcaga atttaacctc
    67081 ggtgaagagc tgttcagcgc ggtagatgaa acctggcaga gcctgaaaga caccttcagc
    67141 cttagcgtac tgatgaatcc cattgaagct agcaaaggcg acggcgaaat ggggaccggg
    67201 gcgatgggcg tgatggatca gaaattcggt agcgcagctg ccgcatacag ctacctgatt
    67261 ttcgtcctgc tgtatgtacc gtgcatctcg gtgatggggg ctatcgcccg tgaatcaagc
    67321 cgtggctgga tgggcttctc catcctgtgg gggctgaata tcgcttactc actggcaaca
    67381 ttgttctatc aggtcgccag ctacagccag cacccaactt acagcctggt gtgcattctg
    67441 gcggttatcc tgtttaacat cgtggttatc ggtttgctgc gtcgcgcgcg tagccgggtg
    67501 gatatcgaac tgctggcaac gcgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc
    67561 ggtgattgcc attaatggct tcacttattc aggtgcgtga tttgctggcg ttacggggcc
    67621 gtatggaagc ggcccagata agccagacat tgaacacccc gcagccaatg attaacgcca
    67681 tgctgcaaca gctggaaagc atgggcaaag ccgtgcggat tcaggaagaa cctgacggct
    67741 gcctctctgg caattgtaaa agctgcccgg aaggcaaagc ctgcctgcgc gagtggtggg
    67801 cgctgcgtta accatactcc atcacctgtt ttgaaaagcc cggtatgcgt ttgcatccgg
    67861 gcttttttgc gtgcggcttt ccataaaaat gcaacccttg cagcgcgacg taaattcctc
    67921 tgaaagcgtc tcgcagggat gaaaactcgc taatgcacag gtgtggagtg gtgcgtagag
    67981 tcgcggcatt caaacaacag gtgaaggaac gccatgagca aaaagcagag ttccacccca
    68041 cacgatgcgc tgttcaaact ctttttacgc caaccggaga cggcgcgcga ttttcttgcg
    68101 tttcatttac cggcacctat tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg
    68161 agcagcttta ttgatgacga tctgcgtgaa agctattccg acgtgctgtg gtcggtgaaa
    68221 acggaacaag ggccaggata tatctattgt ctgattgaac atcaaagcac ctcaaacaaa
    68281 ctgatcgcat ttcgcatgat gcgttacgcc attgccgcaa tgcaaaatca cctggatgcc
    68341 ggatacaaaa cattgccgat ggtggtgcca ttgttgtttt accacggtat tgaaagcccc
    68401 tatccctatt cgctgtgttg gcttgattgt ttcgccgatc ccaaactggc aaggcagctt
    68461 tatgcctccg catttccgct gattgatgtc accgtcatgc ctgatgatga aatcatgcag
    68521 caccgacgta tggcgctgct ggagttaatt caaaaacata ttcgtcaacg cgatctgatg
    68581 gggctggtag agcaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc
    68641 aaagggctgt ttaattacat cttgcaaacc ggcgatgcgg tacgttttaa cgattttatc
    68701 gacggcgttg ccgaacattc accgaaacac aaggagagct taatgactat tgcggaaaga
    68761 ttgcggcaag aggggtttca gaaaggtata cgagctatcg ccaaaaccat gctggatgcc
    68821 ggagtccctc ttgaggacgt tctgcgattt accagcctga cggttgaaga ccttgctgaa
    68881 ataaatcatc aataacgtaa aggatggttt taatgacaga tgcagaaaga gaatatctta
    68941 ttagttacct gattggttat ttattgggtc gacgcctgga agccctggat atcgccaaaa
    69001 gaatgctgta atccggagtc cctcttgcag acatcatgcg ctttaccggg gtgtcagaag
    69061 aagagttggc tgcggcgagc cagtaaagtt ctgtctcgcc atttcaaaag ccacctacac
    69121 cctctgcttc aacgccacca gcatgcgaca aaactcggcc ggatgcgaaa taaacggcgc
    69181 atgggccgct ttggcgaaga tgtatgattc gctgtgaggc caaagtttat ccagcatcgg
    69241 caccactttg cgcggcacca gaccgtcgag atagccatac aatcgcaaaa acggcatgga
    69301 cacgttttgc agcggctgac ggagatcgac cattttcagg atttccagcc cgccattaag
    69361 cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcagcgccc gcgcatcctg
    69421 gcgcgccgtt tcagtaccca tggtttgtaa cgccaggaac cgctccaccg tacgctgaaa
    69481 atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc ccggccactc
    69541 gtcacgagca ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg
    69601 atgggttaac gcaatctggc ttgccaccag cccgcccaga ctccagccta accaaatggc
    69661 tttatcaggt gcctgtcgca gcacggcttc ggccatatca gcaagtgaca tcgcaccaaa
    69721 tccccggcta cgcccgaagc cgggcagatc aacaaggtgc agcgtaaaat gcgagctcag
    69781 ttcctcgtca atgcaacgcc acacttcggc attcagtccc catccgtgca gcagcacaag
    69841 atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct attgttctct
    69901 tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc tatgccgaat
    69961 gccgctggcg ttaggtcatt gggggatttg ttcggtatgc tcacgcgccg cccgtacaga
    70021 taaaacgtta tgcccccaat gtggattacc cgccacacac tcacatcttc cctgcggtcg
    70081 ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg gttgccgact atgcgccgcc
    70141 gttaagtccg cttatccacc agcttaagtt ttcccggcgc agcgaaatcg ccagcgccct
    70201 ggcacgtctg ttactattgg aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc
    70261 ggatcgcatt atcagcgttc cgttatggca gcagcgtcac tggcgtcggg gatttaatca
    70321 gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac tgccgatggg atagcgaagc
    70381 cgtcacacga acacgggcca ctgcgaccca gcattttctc agtgcccggc tgcgcaagcg
    70441 caacctgaaa aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata tggtgattgt
    70501 ggatgatgtc gtcaccaccg gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa
    70561 tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg
    70621 gcgtattata accaactaaa atagtcaact attaggccat tactatgatc cgtatttccg
    70681 atgctgcaca agcgcacttt gccaaactgc tggcaaatca ggaagaaggg acacaaatcc
    70741 gcgtatttgt gattaaccct ggcacgccta acgctgaatg tggcgtttct tattgtccgc
    70801 cggacgctgt agaagccacc gacactgccc tgaaatttga cctgctgacc gcgtatgttg
    70861 atgagttaag cgctccttac ctggaagatg cagagatcga ctttgttaca gaccagttgg
    70921 gttcccagtt aacgctgaaa gcgcctaacg ccaaaatgcg taaagtcgca gacgatgcac
    70981 cgctgatgga gcgcgtggag tatatgctgc agtcgcagat caacccacag cttgctggtc
    71041 acggtggtcg cgtttcgctg atggaaatca ccgaagacgg ttacgccatt ctgcaatttg
    71101 gcggcggctg taacggttgt tctatggtag atgtgacgct gaaagaaggg atcgagaagc
    71161 agctgctgaa cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg
    71221 gcgagcactc ctactactaa gttatcccct cttttgtaga ttgcccgatg cgacgctaaa
    71281 gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa
    71341 acccctccct tcgtttcccc gcgataatat gaccaacctc tcataattta aatttacccc
    71401 gctctggtga ttctcaaacg ccggatgtta cccgtatcat tcacgtgggt accaaacata
    71461 ctcctgacat ctgactacaa taattagttt tagtgggtat cagtcgtggt gccgcaatat
    71521 ctctgttccc gattgggata attagagttt gtcgtcagaa aattgacgtt acccataaca
    71581 aatgaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttgt
    71641 tgctcctgat gatccgtttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg
    71701 ctgttggatt aatgcaagga atgccgctgg ataaagttat tggctccatc aaagcaggtg
    71761 tcggcggaac gctcggtagc cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa
    71821 tgctggcaga ctgcggtggc gcacaacgta tcgccaccac gctgattgct aaatttggta
    71881 aaaaacacat ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct
    71941 atgaagtggg ctttgtgctg atgctgccgc tggtgtttac catcgcggct tccgcgaata
    72001 ttccactgct gtatgttggt gtaccaatgg cggctgcact gtctgtgacc cacggcttcc
    72061 tgccaccgca tccgggcccg actgcgattg ccaccatttt caatgccgat atgggtaaaa
    72121 ccctgctgta cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg
    72181 ctcgcgtgct gaaaggtata gataagccga tcccggaagg tctgtacagc gcgaaaacct
    72241 tcagcgaaga agagatgcca agttttggcg tcagcgtctg gacctctctg gtcccggttg
    72301 tgctgatggc gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc
    72361 cggtagcgga gttcctcggt gacccggtaa tggctacgct gattgccgta ctgattgcga
    72421 tgttcacctt tggtctgaac cgtggtcgtt caatggatca gattaacgac acgctggttt
    72481 cttccatcaa aatcattgcg atgatgctgt tgattatcgg tggtggcggt gcgttcaagc
    72541 aggtgctggt agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca
    72601 tttctccgct gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg
    72661 caaccgttgc ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg
    72721 ttagcccgga gctgatggtt attgcggttg gttccggtag tgtgattttc tctcacgtga
    72781 acgatccggg cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca
    72841 aatcctggtc gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc
    72901 tgaatatggt gatttgataa cactgactgc cggatgcatc gcatccggca tcaaccgcac
    72961 tctacttctt cttcgctgcc gctctgcgcc gtctgtccaa atccttcagc aacttgttca
    73021 caccatcatc ggcaaacatc gcctcaagcg ttgcggagag cttgcgccgc cagtttttat
    73081 actggtaact ggtgccggga atatttaccg ggtccgccat atccagccag tcttctggct
    73141 gtagtcctaa cagggcgctg ttgctatcgg cgatgtagcg ctgtaagcca cgattcagcg
    73201 tcggtgtcat cgacatcaac gatgccttat gcccggcacg ttttggcagg caaccatatt
    73261 tatgcagagc atccagtagt ccttgcttcg ccagttcgcg atcctgatac agaccgcgca
    73321 gtaccacttc atccggatac agccccagcg ttttgcccag cgttaaatcc ccgctctccc
    73381 agtagccgcg tagcgtaggc aggtcgtgtg tcgccgcaac cgccatcgac tgctctgggt
    73441 acgctttcgg tgcgcggaat gtcttctcgt ggtcgttttc gaaatagagc actttgtagg
    73501 agtacacacc gctgctgcgc agcttaccga caatctctac cggtacggta ccgagatctt
    73561 caccaatcac catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat
    73621 ccaccggata gtgaacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca
    73681 aacgcagcat cgacatcacg tgatcaattc gtaatgcgcc gcagttttgc atattggcac
    73741 gcaacagctc gataaacggt tcataggcac gcgcggtgat aatatgcggg tccattggcg
    73801 gcaatcccca gttctgcccc aacgggccga ggatatccgg cggtgctcca accgacgctt
    73861 tcaggcaata caactcccgg tcgcaccagg tttctgatcc accctctgct acaccaaccg
    73921 ccagatcacg atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg
    73981 caaactggct gtaagccagc cactgcaacc agagataaaa atcgacgtca tcacgatgtt
    74041 cttcgcagaa ctggcgcact tctggtgaat ccacgttctg atacatctct ggccatgcag
    74101 gccagcccca gcgcatttcg tcctctttca cttgctgggc atgtagcgcg tcaaacgccg
    74161 cctgccagaa caggctatcg ccttgctcgg caacaaactg acgaaacgcg gccacctgtt
    74221 catcatcacg ttgcgcgaaa cctttccacg ccatgcgcaa tgctgtcatt tttagggcgg
    74281 taaccgtgga gtaatcgacc cagtcggcat cccgcgtctg ttgcagcgtc tgttgcgtgg
    74341 tcggcaactt ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg acggcgttaa
    74401 cgtcgatata aatcacattc agccaacggc gagaagacgg gctgtatggg ctggcacttt
    74461 ccggatttgc cggatagagc gcatgaatcg ggttcagacc gatgaacgac ccgccacgtt
    74521 ttgccacatc caccagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt
    74581 ccgatcgcag cgtataaagc tgaacgcagg caccccacag cttttgttta ttcagcaacg
    74641 cctgcggttc gtaacagcgt ttcggggcga caatcaccct gcaatgcacg cgctggtctt
    74701 cctgggtaaa cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt
    74761 tgccccccgt tacatggcct ttgtactgcg ttccttcttc ggtggtcagc agccagctat
    74821 attcgccgct gccctccacc accatcggca tttttttgcc gctggtataa accatgacat
    74881 tcgggactgg cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac
    74941 gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taattggggc
    75001 taatccccgc cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc
    75061 gttttgcctg ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac
    75121 cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca
    75181 catccacctg cttttgtgcc tctacatagg ctgcgaaatc cgccatcacc agatacggat
    75241 cgccgccctg tttgccgata ctgtgcagca tctgatcgaa ggcatgctta tcgccgtcgc
    75301 tgtatttgcc gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc
    75361 atttcaccgg gtcgtagcct ttggccagaa ttgccttcac ttgttccacg gtatgaccaa
    75421 aaataaagat attttcttca ccaactttct cggcgatttc aacgttcgca ccatccagcg
    75481 taccgacagt aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt
    75541 tacctgccgt cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg
    75601 aaacgcaata atccggcagg aacaccacct tcaatttgtc gccaacctgc ggatcgttgt
    75661 tgatcacgtc agccactttg ttgatcgcaa agataatgtt tttcgccagg tagtagcccg
    75721 gtgccgcttt cgcgccaaag aggaagacgc gcggtacgcg atcagcctgc gggttttcac
    75781 gaatttcttt gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat
    75841 gcagacgttt gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa
    75901 ctttcacaaa ctctgccaga cggactttat tcgcctgctt gatctcgcga tattgctgac
    75961 ggaatttcgc atcatcagcg aatttttcca gattgatcag ttgatcgaga tcgttagtcc
    76021 actctttttt cagtgattta tccaacagag ccgccagtgc cgggttgcac tgtttgatcc
    76081 agcgacgtgg ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat
    76141 attccgggaa cagatctttc accaccagat ccgagtgcag ggccgcaaca ccgtttaccg
    76201 cgaaaccgcc aaccacacac aggttcgcca tatgcacttg tttgtcgtgt accaccgcca
    76261 gtttggccca tactttttca tcgcccggcc aggttttttc caccagcgtt ttaaagcgtg
    76321 tattaatttc gttaataatc tgcatgtggc gcggcagtaa gcctttcacc agtttcacat
    76381 cccagcgttc cagcgcttct ggcatcaagg tatggttggt gtaagcgaaa gttttactgg
    76441 tgatggccca ggcatcatcc cagctcatct ggtgctcatc gatcagcacg cgcagcagtt
    76501 ctggaatcgc gatagttggg tgggtatcgt tcagctgaat aacttcgtac tccgccagct
    76561 cgtgcagttt acgccccgcc agatgatggc gacgcaaaat atccgctacc gagcaggcac
    76621 actggaagta ttgctgcatc aggcgcagct ttttaccggc agtatggttg tcgtttggat
    76681 agagaacttt ggtcagtttt tccgcgttga tgccctgctg ttcggcacgc aggaaatcac
    76741 catcgttaaa tttagtcaga tcaaacggat gcgcatgcgt cgcctgccac agacgcagcg
    76801 gctgcgccac gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg
    76861 taaactccgg ctcccagcgt ccgtctttcg tcactttacc gccaatccct acctgcacat
    76921 ccagtgcttc gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt
    76981 caacctgttt gccatcgaca aatgactggc ggaacaagcc atattgataa ttaaggccgt
    77041 aaccagtagc tgactgcccg acagttgcca ttgagtcgag gaagcacgcc gccagacgtc
    77101 ccagaccacc gttacccagc gccgggtcga tctcttcttc cagcaagtca gtcaggttga
    77161 tgtcataagc cttcaacgaa tcctgtacat cctgatacca gccgagattc aacagattgt
    77221 tgcccgtcag gcgaccaatc aaaaactcca ttgagatgta gttaacatgc cgctgattcg
    77281 ccaccggttt ggcaaatggc tgagcacgca gcatttcggc cagtgcttcg ctcactgcca
    77341 accaccactg acgaggggtc atttcagccg cagaatttaa gccataacgc tgccactgac
    77401 gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac
    77461 ttttcttaga ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc
    77521 tcatcctgcg ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc
    77581 gaccacttaa agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag
    77641 aaaatctgta tcaaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac
    77701 tctataatta agtaaaggag tgaaacagtt tcacaagtaa aatatccagt gtgctccatc
    77761 tcattcttaa tagatttatt aagatcatct ttttagatgg cactttcatc agaaatgaag
    77821 aagaaaccct tgcttaaatg aaactgatga acataaggaa aataccgtac aacccagtat
    77881 tcacgctgga tcagcgtcgt tttaggtgag ttgttaataa acatttggaa ttgtgacaca
    77941 gtgcaaattc agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac
    78001 cttataacca ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa
    78061 gtgattaact atgctgattc cgtcaaaatt aagtcgcccg gttcgactcg accataccgt
    78121 ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat
    78181 cacaagtcct gcgggctacg gaaagaccac gctcatttcc cagtgggcgg caggcaaaaa
    78241 cgatatcggc tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta
    78301 tctcattgcc gccgtgcaac aggcaaccaa tggtcactgc gcgatatgtg agacgatggc
    78361 gcaaaaacgg caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc
    78421 ggaatggcat agcccacttt atctggtcat cgatgactat catctgatca ctaatcctgt
    78481 gatccacgag tcaatgcgct tctttattcg ccatcaacca gaaaatctca cccttgtggt
    78541 gttgtcacgc aaccttccgc aactgggcat tgccaatctg cgtgttcgtg accagctgct
    78601 ggaaattggc agtcagcaac tggcatttac ccatcaggaa gcgaagcagt tttttgattg
    78661 ccgtctgtca tcgccaattg aagccgcaga aagcagtcgg atttgcgatg acgtttccgg
    78721 ttgggcgacg gcactgcagc taatcgccct ctccgcccgg cagaatactc actcagccca
    78781 taagtcggca cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga
    78841 tgaggttttg gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt
    78901 gcgctcaatg aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg
    78961 cctcgaagag attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgattg
    79021 gttttgctat cacccactgt ttggtaactt cctgcgccag cgctgccagt gggaactggc
    79081 ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc
    79141 cagcgaagcg attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct
    79201 gcttaatcac gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct
    79261 taaggccctg ccgtgggaca gtttgctgga aaatccgcag ttggtgttat tgcaggcgtg
    79321 gctgatgcaa agccagcatc gctacggcga agttaacacc ctactggccc gcgctgaaca
    79381 tgaaatcaag gacattagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca
    79441 ggtagcgatt aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga
    79501 agagctgccg ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt
    79561 gctgcactgc aaaggcgaat tgacccgctc actggcgcta atgcagcaaa ccgaacagat
    79621 ggcacgccag cacgacgtct ggcactacgc cttatggagt ttaatccagc aaagtgaaat
    79681 tctgtttgct cagggattcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct
    79741 gatcaacgag cagcatctgg aacagctgcc aatgcatgag tttctggtgc gcatccgagc
    79801 acaactgtta tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat
    79861 tgaagtcttg tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca
    79921 atgctcgctg gcccgtggtg atttagataa cgctcgtagc cagcttaacc gtctggaaaa
    79981 cctgctgggg aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt
    80041 gatttactgg caaatgacca gcgataaagc cgccgctgcc aactggttgc gtcatacggc
    80101 taaaccggag tttgcgaaca accacttcct gcaaggtcaa tggcacaaca ttgcccgtgc
    80161 acaaatcttg ctgggcgagt ttgaaccggc agaaattgtt ctcgaagaac tcaatgaaaa
    80221 cgcccgcagt ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact
    80281 gtactggcag gccggacgta aaagtgacgc ccagcgtgtg ttgctggacg cattaaaact
    80341 ggcgaatcgc accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca
    80401 actgcgccag cttattcaac tcaatacgct gccggagctg gaacagcatc gcgcacagcg
    80461 tattctgcga gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt
    80521 cgttgaacgt ctgctaaatc atccggaagt gccagaactg atccgcacca gcccgttgac
    80581 ccaacgtgaa tggcaggtcc tggggctgat ttactccggt tacagcaacg agcaaattgc
    80641 aggtgagctg gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa
    80701 actcggtgtc gcccaccgtc aggccgcggt acaacacgcg cagaaactgc tgaagatgat
    80761 gggatatggg gtgtgagttt agccggataa tacgtcggat ccggcttaca tctttatatc
    80821 attcaatgct cacccgcgtt acgccatccg cctccaccag actaaaccgc actggcaaga
    80881 aacgctccac caccgcaata ttggtcagca gatggcatga gggatgggcg accgtaaatt
    80941 ctcccgcgcc cgccagcgcc atcggcagta ccagctggtc ggcgagatat tcccccaccg
    81001 ccgccggact tgccaggtag cgtttcacct ctttcaccaa ctgtgccgcg accacttcgg
    81061 cgctgacgcg cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt
    81121 caagcgaaac ggtattaccc ggtccctggt cacgcggcag gttatgaata ttctgctcat
    81181 gcagggaaaa actccccgcc agtgtagcga tttcacgctc agcgacatgg cgcggcacgc
    81241 cagctaatag cacctctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg
    81301 tgttaaacaa tgccaccggc gagacttccg ttgccaccac cccgcctcca gcaggataaa
    81361 aaccgtggcg taataatgtg gtttgctgat gaatgcccat tttcgccagc agcggctcca
    81421 gcacccggcg gataaaatcc gcaggcggtg ccgacgggtt atcggtgccg ccgctcactt
    81481 caacacgcga aggcccatca gcaaaccaca gcgcgggtag caccgtttgc agcaccagcg
    81541 tgctgcttcc ggcactaccg atagcaaagc ggtaatcgcc gccacgcacg gtaccgggcc
    81601 ggaagaccag acgctgtgac cccagctccg cgccttccac cgttgcccta caaatttccg
    81661 cagccgcttt taccgcggtc agatgttggc gcaacagccc cggtttcgcc cgcccggcac
    81721 gaatgccggt gatggtaaat ggctggccgg ttatcatcga caggctcagc gccgagcgca
    81781 ggatctgccc gccgccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt
    81841 atccttttac gcacaccacc tgacgcaggg tatagataac ttccaccaga tcgctttgtg
    81901 ccgccatcac cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acttcggcat
    81961 ctttacggca ttccacatgc gcggtggcac gaatttgatc ttccacgctg aacatttttt
    82021 tcgctttagt acggctcata acccgcccgg caccgtggct gcacgaacag aacgactctt
    82081 catttcccag cccacggacg ataaagcttt tcgctcccat cgaccctgga ataattccat
    82141 attgaccagc acgcgcagac accgcgcctt tacgcgtcac gtagatctct tcaccaaagt
    82201 gctgttcttt ttgcacatag ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg
    82261 gctgtctgac cgttttctgc gtaatgctct gcaacgccgt taccacgttt tccatcatcg
    82321 catcgcggtt aaggctggca aaaagctgcg cccaggccac ggctttcagg taatcatcaa
    82381 agtattccgt accttccata aagtacgcca gatcacgcga cggcaatgtt tcaagcgttt
    82441 cctgcatctc tttttgcgcc agatcgataa agtaagtccc gatggcgtta ccaattccgc
    82501 gtgaaccgga gtgcagcata atccacacct ggtccgactc atcaaggcag atttcaataa
    82561 agtggttacc cgttcccagc gttcccaggt gtttatagtt gttagtgtta aggaaacggg
    82621 gatatttttg cgttaaccac tgataaccgg cttccagctc agcccatttc gcatcgacgt
    82681 taacaggcgg attttcccat gcgcctttat cacgtttaca acgtccggta gtacgtccgt
    82741 gcggcacggc tgtttcaatc gcctgacgca gctccgccag gttttccggc aggtctgccg
    82801 ccgttaacgc ggtacgcagc gcgttcattc cacaaccgat atccacgccc accgccgccg
    82861 gaataatcgc ccctttggtc gggatcacgc taccaatggt ggaaccttta cccaggtgta
    82921 catccggcat taccgcaata tgtttgaaaa taaacggcat cttcgccgta ttaataagtt
    82981 gctgacgcgc atcggcctct accggcacgc ctttggtcca catttttacc ggggcatttt
    83041 cagtggtcag taattcgtaa ttcattttgt ttttctcttt tcgttgtttg ctgtcctgat
    83101 aattgcaacc gtcgtgccag aaaattaaat aagcagctta atttttttat tta
//
LOCUS       DAZGTJ010000010        80020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000010 DAZGTJ010000000
VERSION     DAZGTJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.010"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            226..2073
                     /gene="ldtD"
                     /locus_tag="ACHK8E_000913"
     CDS             226..2073
                     /gene="ldtD"
                     /locus_tag="ACHK8E_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0343217.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2254..2802
                     /gene="mepK"
                     /locus_tag="ACHK8E_000914"
     CDS             2254..2802
                     /gene="mepK"
                     /locus_tag="ACHK8E_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP0343218.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2829..3476
                     /gene="gloC"
                     /locus_tag="ACHK8E_000915"
     CDS             2829..3476
                     /gene="gloC"
                     /locus_tag="ACHK8E_000915"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP0343219.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHK8E_000916"
     CDS             complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHK8E_000916"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP0343220.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHK8E_000917"
     CDS             complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHK8E_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP0343221.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6592..7992)
                     /gene="asnS"
                     /locus_tag="ACHK8E_000918"
     CDS             complement(6592..7992)
                     /gene="asnS"
                     /locus_tag="ACHK8E_000918"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP0343222.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8161..9363)
                     /gene="pncB"
                     /locus_tag="ACHK8E_000919"
     CDS             complement(8161..9363)
                     /gene="pncB"
                     /locus_tag="ACHK8E_000919"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP0343223.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9629..12241
                     /gene="pepN"
                     /locus_tag="ACHK8E_000920"
     CDS             9629..12241
                     /gene="pepN"
                     /locus_tag="ACHK8E_000920"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP0343224.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12447..13214)
                     /gene="ssuB"
                     /locus_tag="ACHK8E_000921"
     CDS             complement(12447..13214)
                     /gene="ssuB"
                     /locus_tag="ACHK8E_000921"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0343225.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(13211..14002)
                     /gene="ssuC"
                     /locus_tag="ACHK8E_000922"
     CDS             complement(13211..14002)
                     /gene="ssuC"
                     /locus_tag="ACHK8E_000922"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP0343226.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14014..15159)
                     /gene="ssuD"
                     /locus_tag="ACHK8E_000923"
     CDS             complement(14014..15159)
                     /gene="ssuD"
                     /locus_tag="ACHK8E_000923"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP0343227.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(15156..16115)
                     /gene="ssuA"
                     /locus_tag="ACHK8E_000924"
     CDS             complement(15156..16115)
                     /gene="ssuA"
                     /locus_tag="ACHK8E_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP0343228.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16108..16683)
                     /gene="ssuE"
                     /locus_tag="ACHK8E_000925"
     CDS             complement(16108..16683)
                     /gene="ssuE"
                     /locus_tag="ACHK8E_000925"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP0343229.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16920..17930
                     /gene="pyrD"
                     /locus_tag="ACHK8E_000926"
     CDS             16920..17930
                     /gene="pyrD"
                     /locus_tag="ACHK8E_000926"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP0343230.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            18104..18646
                     /gene="zapC"
                     /locus_tag="ACHK8E_000927"
     CDS             18104..18646
                     /gene="zapC"
                     /locus_tag="ACHK8E_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP0343231.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(18643..19752)
                     /gene="ycbX"
                     /locus_tag="ACHK8E_000928"
     CDS             complement(18643..19752)
                     /gene="ycbX"
                     /locus_tag="ACHK8E_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP0343232.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            19996..22104
                     /gene="rlmKL"
                     /locus_tag="ACHK8E_000929"
     CDS             19996..22104
                     /gene="rlmKL"
                     /locus_tag="ACHK8E_000929"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP0343233.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            22116..24023
                     /gene="uup"
                     /locus_tag="ACHK8E_000930"
     CDS             22116..24023
                     /gene="uup"
                     /locus_tag="ACHK8E_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0343234.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            24153..25406
                     /gene="pqiA"
                     /locus_tag="ACHK8E_000931"
     CDS             24153..25406
                     /gene="pqiA"
                     /locus_tag="ACHK8E_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP0343235.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            25411..27051
                     /gene="pqiB"
                     /locus_tag="ACHK8E_000932"
     CDS             25411..27051
                     /gene="pqiB"
                     /locus_tag="ACHK8E_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP0343236.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            27048..27611
                     /gene="pqiC"
                     /locus_tag="ACHK8E_000933"
     CDS             27048..27611
                     /gene="pqiC"
                     /locus_tag="ACHK8E_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP0343237.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            27867..28034
                     /gene="rmf"
                     /locus_tag="ACHK8E_000934"
     CDS             27867..28034
                     /gene="rmf"
                     /locus_tag="ACHK8E_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP0343238.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(28104..28622)
                     /gene="fabA"
                     /locus_tag="ACHK8E_000935"
     CDS             complement(28104..28622)
                     /gene="fabA"
                     /locus_tag="ACHK8E_000935"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP0343239.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(28691..30451)
                     /gene="ycbZ"
                     /locus_tag="ACHK8E_000936"
     CDS             complement(28691..30451)
                     /gene="ycbZ"
                     /locus_tag="ACHK8E_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0343240.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            30637..31089
                     /gene="matP"
                     /locus_tag="ACHK8E_000937"
     CDS             30637..31089
                     /gene="matP"
                     /locus_tag="ACHK8E_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP0343241.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(31165..32217)
                     /gene="ompA"
                     /locus_tag="ACHK8E_000938"
     CDS             complement(31165..32217)
                     /gene="ompA"
                     /locus_tag="ACHK8E_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP0343242.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(32573..33082)
                     /gene="sulA"
                     /locus_tag="ACHK8E_000939"
     CDS             complement(32573..33082)
                     /gene="sulA"
                     /locus_tag="ACHK8E_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP0343243.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            33300..33929
                     /gene="sxy"
                     /locus_tag="ACHK8E_000940"
     CDS             33300..33929
                     /gene="sxy"
                     /locus_tag="ACHK8E_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP0343244.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(33892..36054)
                     /gene="yccS"
                     /locus_tag="ACHK8E_000941"
     CDS             complement(33892..36054)
                     /gene="yccS"
                     /locus_tag="ACHK8E_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP0343245.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(36064..36510)
                     /gene="yccF"
                     /locus_tag="ACHK8E_000942"
     CDS             complement(36064..36510)
                     /gene="yccF"
                     /locus_tag="ACHK8E_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP0343246.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            36633..38687
                     /gene="helD"
                     /locus_tag="ACHK8E_000943"
     CDS             36633..38687
                     /gene="helD"
                     /locus_tag="ACHK8E_000943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP0343247.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(38719..39177)
                     /gene="mgsA"
                     /locus_tag="ACHK8E_000944"
     CDS             complement(38719..39177)
                     /gene="mgsA"
                     /locus_tag="ACHK8E_000944"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP0343248.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(39273..39935)
                     /gene="yccT"
                     /locus_tag="ACHK8E_000945"
     CDS             complement(39273..39935)
                     /gene="yccT"
                     /locus_tag="ACHK8E_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP0343249.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            40108..40521
                     /gene="yccU"
                     /locus_tag="ACHK8E_000946"
     CDS             40108..40521
                     /gene="yccU"
                     /locus_tag="ACHK8E_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP0343250.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(40566..40883)
                     /gene="hspQ"
                     /locus_tag="ACHK8E_000947"
     CDS             complement(40566..40883)
                     /gene="hspQ"
                     /locus_tag="ACHK8E_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP0343251.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(40941..42131)
                     /gene="rlmI"
                     /locus_tag="ACHK8E_000948"
     CDS             complement(40941..42131)
                     /gene="rlmI"
                     /locus_tag="ACHK8E_000948"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP0343252.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            42226..42504
                     /gene="yccX"
                     /locus_tag="ACHK8E_000949"
     CDS             42226..42504
                     /gene="yccX"
                     /locus_tag="ACHK8E_000949"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP0343253.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(42501..42830)
                     /gene="tusE"
                     /locus_tag="ACHK8E_000950"
     CDS             complement(42501..42830)
                     /gene="tusE"
                     /locus_tag="ACHK8E_000950"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP0343254.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(42921..43580)
                     /gene="yccA"
                     /locus_tag="ACHK8E_000951"
     CDS             complement(42921..43580)
                     /gene="yccA"
                     /locus_tag="ACHK8E_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP0343255.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(43789..43876)
                     /locus_tag="ACHK8E_000952"
     tRNA            complement(43789..43876)
                     /locus_tag="ACHK8E_000952"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43840..43842),aa:Ser,seq:tga)
     gene            44303..45421
                     /gene="hyaA"
                     /locus_tag="ACHK8E_000953"
     CDS             44303..45421
                     /gene="hyaA"
                     /locus_tag="ACHK8E_000953"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP0343256.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            45418..47211
                     /gene="hyaB"
                     /locus_tag="ACHK8E_000954"
     CDS             45418..47211
                     /gene="hyaB"
                     /locus_tag="ACHK8E_000954"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP0343257.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            47230..47937
                     /gene="hyaC"
                     /locus_tag="ACHK8E_000955"
     CDS             47230..47937
                     /gene="hyaC"
                     /locus_tag="ACHK8E_000955"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP0343258.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            47934..48521
                     /gene="hyaD"
                     /locus_tag="ACHK8E_000956"
     CDS             47934..48521
                     /gene="hyaD"
                     /locus_tag="ACHK8E_000956"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP0343259.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            48518..48916
                     /gene="hyaE"
                     /locus_tag="ACHK8E_000957"
     CDS             48518..48916
                     /gene="hyaE"
                     /locus_tag="ACHK8E_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP0343260.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            48913..49770
                     /gene="hyaF"
                     /locus_tag="ACHK8E_000958"
     CDS             48913..49770
                     /gene="hyaF"
                     /locus_tag="ACHK8E_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP0343261.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            49904..51448
                     /gene="appC"
                     /locus_tag="ACHK8E_000959"
     CDS             49904..51448
                     /gene="appC"
                     /locus_tag="ACHK8E_000959"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP0343262.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            51460..52596
                     /gene="appB"
                     /locus_tag="ACHK8E_000960"
     CDS             51460..52596
                     /gene="appB"
                     /locus_tag="ACHK8E_000960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0343263.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            52609..52701
                     /gene="cbdX"
                     /locus_tag="ACHK8E_000961"
     CDS             52609..52701
                     /gene="cbdX"
                     /locus_tag="ACHK8E_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP0343264.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            52781..54079
                     /gene="appA"
                     /locus_tag="ACHK8E_000962"
     CDS             52781..54079
                     /gene="appA"
                     /locus_tag="ACHK8E_000962"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP0343265.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(54199..56379)
                     /gene="etk"
                     /locus_tag="ACHK8E_000963"
     CDS             complement(54199..56379)
                     /gene="etk"
                     /locus_tag="ACHK8E_000963"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP0343266.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(56399..56845)
                     /gene="etp"
                     /locus_tag="ACHK8E_000964"
                     /gene_synonym="yccY"
     CDS             complement(56399..56845)
                     /gene="etp"
                     /locus_tag="ACHK8E_000964"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP0343267.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(56833..57972)
                     /gene="gfcE"
                     /locus_tag="ACHK8E_000965"
     CDS             complement(56833..57972)
                     /gene="gfcE"
                     /locus_tag="ACHK8E_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP0343268.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(58018..60114)
                     /gene="gfcD"
                     /locus_tag="ACHK8E_000966"
     CDS             complement(58018..60114)
                     /gene="gfcD"
                     /locus_tag="ACHK8E_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0343269.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(60114..60860)
                     /gene="gfcC"
                     /locus_tag="ACHK8E_000967"
     CDS             complement(60114..60860)
                     /gene="gfcC"
                     /locus_tag="ACHK8E_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0343270.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(60857..61501)
                     /gene="gfcB"
                     /locus_tag="ACHK8E_000968"
     CDS             complement(60857..61501)
                     /gene="gfcB"
                     /locus_tag="ACHK8E_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP0343271.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(61608..61913)
                     /locus_tag="ACHK8E_000969"
     CDS             complement(61608..61913)
                     /locus_tag="ACHK8E_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343272.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(62354..62566)
                     /gene="cspH"
                     /locus_tag="ACHK8E_000970"
     CDS             complement(62354..62566)
                     /gene="cspH"
                     /locus_tag="ACHK8E_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP0343273.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            62852..63064
                     /gene="cspG"
                     /locus_tag="ACHK8E_000971"
     CDS             62852..63064
                     /gene="cspG"
                     /locus_tag="ACHK8E_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP0343274.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            63075..63263
                     /gene="ymcF"
                     /locus_tag="ACHK8E_000972"
     CDS             63075..63263
                     /gene="ymcF"
                     /locus_tag="ACHK8E_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0343275.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            63238..63468
                     /gene="ymcE"
                     /locus_tag="ACHK8E_000973"
     CDS             63238..63468
                     /gene="ymcE"
                     /locus_tag="ACHK8E_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP0343276.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            63458..63631
                     /gene="gnsA"
                     /locus_tag="ACHK8E_000974"
     CDS             63458..63631
                     /gene="gnsA"
                     /locus_tag="ACHK8E_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0343277.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(63679..64752)
                     /gene="yccM"
                     /locus_tag="ACHK8E_000975"
     CDS             complement(63679..64752)
                     /gene="yccM"
                     /locus_tag="ACHK8E_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0343278.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(64824..67568)
                     /gene="torS"
                     /locus_tag="ACHK8E_000976"
     CDS             complement(64824..67568)
                     /gene="torS"
                     /locus_tag="ACHK8E_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP0343279.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            67651..68679
                     /gene="torT"
                     /locus_tag="ACHK8E_000977"
     CDS             67651..68679
                     /gene="torT"
                     /locus_tag="ACHK8E_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP0343280.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(68652..69344)
                     /gene="torR"
                     /locus_tag="ACHK8E_000978"
     CDS             complement(68652..69344)
                     /gene="torR"
                     /locus_tag="ACHK8E_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP0343281.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            69474..70646
                     /gene="torC"
                     /locus_tag="ACHK8E_000979"
     CDS             69474..70646
                     /gene="torC"
                     /locus_tag="ACHK8E_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP0343282.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            70646..73192
                     /gene="torA"
                     /locus_tag="ACHK8E_000980"
     CDS             70646..73192
                     /gene="torA"
                     /locus_tag="ACHK8E_000980"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP0343283.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            73189..73788
                     /gene="torD"
                     /locus_tag="ACHK8E_000981"
     CDS             73189..73788
                     /gene="torD"
                     /locus_tag="ACHK8E_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP0343284.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(74043..74348)
                     /gene="cbpM"
                     /locus_tag="ACHK8E_000982"
     CDS             complement(74043..74348)
                     /gene="cbpM"
                     /locus_tag="ACHK8E_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP0343285.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(74348..75268)
                     /gene="cbpA"
                     /locus_tag="ACHK8E_000983"
     CDS             complement(74348..75268)
                     /gene="cbpA"
                     /locus_tag="ACHK8E_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP0343286.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            75421..75522
                     /locus_tag="ACHK8E_000984"
                     /pseudo
     CDS             75421..75522
                     /locus_tag="ACHK8E_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            75803..77044
                     /gene="agp"
                     /locus_tag="ACHK8E_000985"
     CDS             75803..77044
                     /gene="agp"
                     /locus_tag="ACHK8E_000985"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP0343287.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(77082..77309)
                     /gene="yccJ"
                     /locus_tag="ACHK8E_000986"
     CDS             complement(77082..77309)
                     /gene="yccJ"
                     /locus_tag="ACHK8E_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP0343288.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(77330..77908)
                     /gene="wrbA"
                     /locus_tag="ACHK8E_000987"
     CDS             complement(77330..77908)
                     /gene="wrbA"
                     /locus_tag="ACHK8E_000987"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0343289.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            complement(77905..79215)
                     /locus_tag="ACHK8E_000988"
     CDS             complement(77905..79215)
                     /locus_tag="ACHK8E_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0343290.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNILNSRAPDVPQSDQEDEIKLYFSK"
     gene            complement(79268..79552)
                     /locus_tag="ACHK8E_000989"
     CDS             complement(79268..79552)
                     /locus_tag="ACHK8E_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0343291.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(79598..79849)
                     /locus_tag="ACHK8E_000990"
     CDS             complement(79598..79849)
                     /locus_tag="ACHK8E_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0343292.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIS
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(79879..>80020)
                     /locus_tag="ACHK8E_000991"
     CDS             complement(79879..>80020)
                     /locus_tag="ACHK8E_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0343293.1"
                     /translation="NIVGDLLRKSQKEARRAGRKQKRWEEACKALRELNKYRDLINEL
                     AE"
BASE COUNT        19628 a        20122 c        20868 g        19402 t
ORIGIN      
        1 cgaaaactcc gtttctgcac taaaaaagtg gcacattacg gcgcgctttt ctttaaactt
       61 ctttacatta ggttatgtaa aaacgtatcg gcgtttatat actgaagata agcctgatga
      121 gtaacaggct tgctcgtcat actttcgtga gtattggcgt tgtacaggca agtcgtaaaa
      181 taacagcctg gctattcaga gtatgataaa aacagggggc aagggatgtt gcttaatatg
      241 atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg
      301 ttcaatgcgc aggccgatga gcctgaagta atccctggcg acagcccggt ggctgtcagt
      361 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
      421 ttgcctgaag gtgcggctga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      481 tataagccgg tttatcttaa ccagcttcaa ttgttgtatg ccgcacgcga tatgcaaccc
      541 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      601 gccggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      661 gggatggcac gcgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      721 aatattccgg tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      781 ccgccgcttt cggtgattaa ccaatggcag ctggcgctgg ataaaggtca attgcctacg
      841 tttgttgtag gattggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      901 gccttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      961 ggacagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgtac aggcatgttg
     1021 gacgggggac cgaaaattac gctacctggc gatgacacgc caactgacgc ggtagtcagc
     1081 ccatccgctg ttactattga aacagcagaa actaagccga tggacaagca aactacgtct
     1141 cgtagtaaac ctgcgcctgt cgttcgtgcc gcctacgata atgaactggt ggaagccgtt
     1201 aaacgttttc aggcatggca aggattgggg gcagatggtg ctattggccc ggcaacgcgt
     1261 gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt tggcactcaa catccagcga
     1321 ttgcgcttgc tgccaacaga gctttctacc gggatcatgg ttaacattcc agcctattcg
     1381 ctggtctact atcagaacgg caatcaggtg ctggattcgc gagtcattgt cggtcgcccc
     1441 gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg tagtggtaaa cccgccgtgg
     1501 aacgtaccac caactctggc acgcaaagat attctgccga aagtgcgcaa cgatccggga
     1561 tatctcgaaa gccatggcta tacggtgatg cgcggctgga acagcagaga agcgattgac
     1621 ccatggcagg ttgactggtc tacaatcacg gcctcgaatt taccgttccg cttccagcag
     1681 gctccaggcc cacggaactc gctggggcgc tataaattca atatgccgag ttcagaggcc
     1741 atttatttgc atgacacgcc gaaccacaat ctgttcaagc gtgatacacg cgcattgagc
     1801 tcaggctgtg tacgagtgaa taaagcttcc gatctggcga atatgctgtt gcaggatgca
     1861 ggctggaatg acaaacgtat ttctgatgcg ctgaagcagg gcgatacgcg ttacgtcaat
     1921 attcggcagt cgattccggt gaatctctac tatctgacgg cctttgttgg tgcagatggt
     1981 cgtacccagt atcgtacaga tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa
     2041 attgtatcga aagcggaaca attaatcagg taaatgaagc agttcgggga aatcagttgt
     2101 cgtaataatt agcatgattg ggggcaattc tctgcagccc ccgtcactgc tggggttgag
     2161 tcatcttgac gtctgcttta cgggcggtta aggtgcctct tgtgcgccag aagtgcatat
     2221 aaacgataac attgacctgt agacttgatt atcatggaca aattcgacgc taatcgccgc
     2281 aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt
     2341 gcaacactct ctaccccacg cccgcgcatt ttgacactca ataatcttca taccggagag
     2401 tcaatcaaag cggagttttt cgatggcaga ggctatattc aggaagaatt ggcaaaactt
     2461 aaccattttt tccgcgatta ccgcgcgaac aaaataaagt ccatcgaccc aggattattc
     2521 gaccagttgt atcgcctgca agggttgtta ggtacccgca aaccggtgca actcatttcc
     2581 ggttatcgtt ccattgatac caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag
     2641 aaaagctatc acacgaaagg gcaggcgatg gatttccata ttgaaggtat cgcgttaagc
     2701 aatattcgca aagccgcgtt atctatgcgc gcaggtggtg taggatatta cccacgtagt
     2761 aactttgtgc atattgatac cgggccagca cggcactggt agcaattgct taacgaaaca
     2821 ggggcagtat gaactatcgt attattccgg tcaccgcatt ctcccagaac tgttcattga
     2881 tctggtgtga acaaacccgc ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa
     2941 tcaaacagga agttgacgcc agcggcctga cactgatgca gatcctgctg acgcatggtc
     3001 atctggacca cgttggtgca gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg
     3061 gcccggaaaa agaagatgag ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg
     3121 gtctggaaga gtgccagccg ctgacgccgg atcgttggct gaacgaaggc gataccatca
     3181 gcatagggaa tgtggcttta caggtgttac attgccctgg gcatacgccg ggtcatgtcg
     3241 tgttttttga tgatcgggca aagctgctga tttctggcga cgttattttc aaaggcggag
     3301 tagggcgcag tgacttcccg cgtggcgatc ataatcaact gatttcttca atcaaagata
     3361 agttgctgcc actgggggat gacgtgacat ttattccggg tcacggacca ttatccacac
     3421 ttggttatga gcgcctgcat aatcccttcc tgcaagacga aatgcccgtc tggtaaaggc
     3481 gcataaaaaa gcccgctttt taagcgggct tcattgtttt taacgcttac agcactgcca
     3541 caatcgcttc gcacagcgga gccatattat ctggtgtcat cccggccacg tttacgcgac
     3601 cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt tcttttgtca
     3661 ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg cgatttgcgc
     3721 ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc tggcgcatat
     3781 cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg gtggcaacaa
     3841 cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct ttcatttggc
     3901 tgaatgcgcg atcaatggtt tcactgtcag cagcaaccag agtacaagcg ccaacacgct
     3961 cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct ttatgcatag
     4021 ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaac ccctggtaag
     4081 cgaagtcaaa cagcggtaac cagcctttct caacggaaag ttgtgccagt gtctgccatt
     4141 gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac agcaccacgt
     4201 cgccagcctg agcttcgttc aggctgttaa tcagtgcatc gaagtcaagg gtgtgatttt
     4261 ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag acgctcttat
     4321 ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt tttgccagga
     4381 aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg cgagcacgtt
     4441 tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag cgaccaaatt
     4501 cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc agatactgtt
     4561 cagccttttt cacactggtc agtaccgggg ttttgcccgt ctcatcttta tagacaccaa
     4621 tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc aggcccagaa
     4681 tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat tatggttaca
     4741 gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta ttactgatga
     4801 aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa aaaacaggac
     4861 caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg gtaaacgata
     4921 cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc gatctggttg
     4981 atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc gcccacttca
     5041 aagtagttaa ccagatcaac atcaccgata ccttctacgt ctttcgcttt agatttggtg
     5101 taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa cagaacgtct
     5161 tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat tagtgatcgg cgtagcgtta
     5221 cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt cagaccagta
     5281 gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag gttggtacgg
     5341 tcagcggcgc cataagcacc aacgatacca aagccttcgt attcgtagct gatagaaccg
     5401 ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc caggtactga
     5461 acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata ggtagcaacg
     5521 ccgccaacac gaccaacgaa gaagtcatcg ctgtatgccg tgtcaccgcc aaactcaggc
     5581 agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc gtaatcgaaa
     5641 gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt accagtttga
     5701 gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc ataaccggtc
     5761 agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt catgtcacca
     5821 ttgccaccgt aactgttttc accgttaccc ttggagaaat aatgcagacc gacagcttta
     5881 ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt tgcagtacct
     5941 gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat tatttattac
     6001 cctcatggtt tttttatgac acctgccact gccgtcaata agttctgtca ataaaaattt
     6061 acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt tccattcaaa
     6121 ctaacgtttc gctaccgtga aagtgctaca aagataaaga tttggtttca aaaagaaaaa
     6181 atatgtaacc aaaagtaaaa tttaaggaac tttgtgaaca tcgtcatatt tccatatagg
     6241 cgtgatgata tttacagcaa tttttatcta tttatatgat ttccttatat ttaaattaac
     6301 taaacggaaa ttttgtttct gatggaaact ttatcgacct ggcacaaaat cctctttcag
     6361 acatccagaa tgccaaaaaa tagtatgaaa attgtgctat cagctaagaa aaaactaatc
     6421 cgcaataaga atgcgggtta ttttttgtgc gtaaatgttg caatgtaata ttccgtaaca
     6481 ggatgatcgt tatggcgggg ttgcaaatag attgcttgac ttaacaaaca gataacttga
     6541 cagaaaggat aaaaaaagag ccagcgggtg ctggctcttg aagacgctaa attagaagct
     6601 ggcgttacgc ggagtacgtg ggaacggaat cacatcacgc acgttttgca caccagttac
     6661 gtaagcaatc aggcgttcaa aaccaagacc gaaacctgaa tgcggaacag taccgtagcg
     6721 acgcagatcg cgataccacc agtaatcttc tttattcagg cccatttcca gcatacgctc
     6781 gtccagcacg tccagacgtt cttcacgctg ggagccacca atgatctcac cgatgcccgg
     6841 agccagaacg tccatagccg caacggtttt accgtcttcg ttaaggcgca tatagaacgc
     6901 tttaatatct ttcggatagt ttttaaccac taccggtgct ttaaagtgtt cttccgccag
     6961 ataacgctca tgctcagaag agagatcgac gccccagtaa accgggtttt caaacttcct
     7021 gccgcagttt tcgagaatgg tcactgcgtc ggtgtaatcc acctgcgcga aatcggcctc
     7081 aatgaagcgt tccagacgtg aaacggcatc tttatctacg cgttcagcga agaatttcat
     7141 gtcgtcagcg cgttcttcga gaaccgcttt gaagacatat ttcagcatgg cttcagccag
     7201 acccgcgata tcgttcaggt tagcaaacgc cacttccggc tccagcatcc agaattccgc
     7261 caggtgacgg ctggtgttgg agttttcagc acggaaagtc gggccgaagg tataaatttt
     7321 ggacaatgcg caagcgtagg tttcgccgtt caactggcca gatacggtca ggaaagactc
     7381 tttaccaaag aagtctttgt cgaaatccac tttgccctga tcgttacgcg gcagattttc
     7441 cagatccagc gtagaaacac ggaacatttc gcctgcacct tcggtatcag atgcggtaat
     7501 cagtggcgtt gaaacccaga aaaatccctg ctcgttaaag aagcgatgca gcgcctgcgc
     7561 cagcgtatgg cgaacgcgcg cgacggcacc aatcaggttt gtgcgcggac gcaggtgagc
     7621 gacttcacgc agatactcaa tgctgtggcg ttttgccgcc atcgggtaag tgtctggatc
     7681 ttcaacccaa ccagcaactt caaccttgct ggcctgaatt tcaaattgtt gcccctggcc
     7741 cggcgacgcc acgactttac ccgtcacaat gaccgagcag ccggtggtca gacgcaagac
     7801 gtcttcattg taattgggca gagaattatt gatgacagcc tgtacaggat caaagcagga
     7861 accgtcataa acggcgagga aggagatgcc agcttttgaa tctcggcggg tacgtaccca
     7921 tccgcgcacg gtgacttcgc tgtcaacggc tacgcggccc tggagtacgt cggctacagg
     7981 cacaacgctc ataatattct ctctgttaat agtcggaaaa aataaacact tgtccacccg
     8041 taaatggggg gatacctatg ttacctggca tctgcaatca gacaagcaga attcgcaaat
     8101 gcagcgaaag atttcggaat taaaggtaaa aaaagggagc cgattagctc cctgatgata
     8161 ttaactggct tttttaatat gcggaaggtc gaacgctttg cgtagcgccc gaacaaacgc
     8221 tttatcgtgg cagatagttt tgcctgggct gtcagaaagt ttcgccactg gtttaccgtt
     8281 acactctacc aacttaatga caatattcag gggttttacc tgggggatat cgcaggtcag
     8341 gcgagtccca ataccaaaac ttaattgcac gcgggaagag aagtggcggt atagctcaac
     8401 cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt ttactctgtg gatcaattcc
     8461 cagcttttca taatgtgcaa tggctttttc accccattca accgggtcac cagagtcatg
     8521 gcgcaggccc tgatagcgac tggcgaactc gacaccgaaa tctcgcagga aagcatccat
     8581 agtgatgcag tcggttaacg caatgccaag ttggtcggga tactcttcca gccaggcagc
     8641 aagtgcagct cgctggctgt tggctagatc cgggctgatt tgctgatgcg cctggaacca
     8701 ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga cgcgccagat cgtagttgct
     8761 ggtgcccacg aaccaggatt cctgttgcag acgtttcacg atggtttctt gtacttcacg
     8821 agaaaaacgt cggcgggtgc caaaatccat cagatggaag cgcgacatat caagaccggc
     8881 ggttaatgcc gagaagtcgg ctaatttgct ttccagcgtg tcgagggctt gcgcaacgtc
     8941 ggcctgcggt gagcgatagc gatgtaccat ttcactgata accgccagca aaggaacttc
     9001 ccagaggatg acttcacgcc acgggccgct taaacgaata tccagcttgc cattatcgtt
     9061 ggacacggtg acttgttccg ggttaaagcg gaactcgcgt agccagttaa gatagtcggc
     9121 cttaaagaaa ggcagggcag agagccactg atattcatca tcctgcaggc gcaggtactg
     9181 catcgcctga acctgttcac gaatagcatc ggcataaata cccagcagat cgtcacctcg
     9241 gcaacgaaac tccgccgcga catgcacatc gtaatagtga tgaaacacgg cttgctgcat
     9301 atgcaactta taagcatctg tatccagcaa cgagtgcaga acaggagaag cgaattgtgt
     9361 cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt acaataaaca tcttcaggaa
     9421 ctgctggagt ataccttgtt tcgcaattta ttgaaccccg atcacaccat atgccacctt
     9481 tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt agcaataaag gcgtttgtac
     9541 ctgaaaagat gaagattctg catagcgcga tttacgcaac aggaatagac tgaacaccag
     9601 actctataaa agatgctaaa ggttatttat gactcaacag ccacaagcca aataccgtca
     9661 cgattatcgt gcgccggatt accagattac tgatattgac ttgacctttg acctggacgc
     9721 gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt catggtgcat cagatgttcc
     9781 gcttcgtctc aacggcgaag acctcaaact ggtttctgtt tatattaatg atgagccgtg
     9841 gaccgcctgg aaagaagaag agggcgcact ggtcatcagt aatttgccgg agcgttttac
     9901 gcttaagatc gttaatgaaa ttagcccggc ggcgaatact gcgctggaag ggctttatca
     9961 gtcaggcgat gcgctttgca cccagtgtga agccgaaggt ttccgccata ttacgtatta
    10021 tctcgaccgc ccggatgtgc tggcgcgttt taccaccaaa attattgccg ataaaaccaa
    10081 atatcccttc ctgctttcca acggtaaccg cgttgcgcaa ggtgaactgg aaaatggacg
    10141 tcattgggta cggtggcagg acccgttccc gaaaccgtgc tacctgtttg cgctggtggc
    10201 aggcgacttt gatgtgctgc gcgacacttt caccacgcgt tctggtcgcg aagtagcgct
    10261 ggagctgtat gtcgatcgtg gcaaccttga tcgtgcgccg tgggcaatga cctcgctgaa
    10321 aaactccatg aaatgggatg aagaacgctt cggcctggag tatgacctcg acatctatat
    10381 gatcgtcgcg gtggatttct tcaatatggg cgcaatggag aataaggggc tgaatatctt
    10441 taactccaaa tatgtgctgg cccgcaccga caccgccacc gacaaagatt acctcgatat
    10501 tgaacgcgtt atcggccatg aatatttcca taactggacc ggcaaccgtg tcacctgtcg
    10561 cgactggttc cagctcagtc tgaaagaagg tttaaccgtc ttccgcgatc aggaattcag
    10621 ctctgacctt ggttcccgcg cggtaaaccg tatcaataat gtgcgcacca tgcgcggatt
    10681 gcagtttgca gaagacgcca gcccgatggc gcacccaatt cgcccggata tggtcattga
    10741 aatgaacaac ttctacaccc tgaccgttta cgagaagggc gcggaagtga ttcgtatgat
    10801 ccacactctg cttggcgaag aaaacttcca gaaagggatg cagctctatt tcgagcgtca
    10861 tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg atggaagatg cgtcgaatgt
    10921 cgatctttcc catttccgcc gttggtacag ccaatccggc acgccggttg tgactgtcaa
    10981 agacgactac aatccggaaa ctgagcagta caccctgacc atcagccagc gcacgccagc
    11041 tacgccggat caggcagaaa aacagccgct gcatattccg tttgccatcg aactgtatga
    11101 taacgaaggc aaagtgatcc cgttgcagaa aggcggtcat ccggtgaatt cggtgctgaa
    11161 cgtcacccag gctgaacaga cctttgtttt tgataatgtc tacttccagc cggtgcctgc
    11221 gctgctgtgc gaattctctg cgccagtgaa actggaatat aagtggagcg atcagcaact
    11281 gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt tgggatgcgg cgcaaagcct
    11341 gctggcaacc tacatcaagc taaacgtcgc ccgtcatcag caagggcagc tgctgtctct
    11401 gccggtgcat gtggctgacg ctttccgcgc ggtgctgctc gatgagaaga ttgatccagc
    11461 gctggcggca gaaatcctga cgctgccttc tgtcaatgaa atggctgaac tgttcgatat
    11521 catcgacccg attgctattg ccgaagtacg cgaagcactc actcgtactc tggcgactga
    11581 actggcggat gagctactgg ctatttacaa cgcgaattac cagagcgagt accgtgttga
    11641 gcatgaagat attgcaaaac gcactctgcg taatgcctgc ctgcgcttcc ttgcttttgg
    11701 tgaaacgcat ctggctgacg tgctggtgag caagcaatac catgaagcaa acaatatgac
    11761 cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag ctgccttgcc gtgacgcgct
    11821 gatgcaggag tacgacgaca agtggcatca ggatggtctg gtgatggata aatggtttat
    11881 cctgcaagcc accagcccgg cggcgaatgt gctggagacg gtgcgtggcc tgttgcagca
    11941 tcgctcattt accatgagca acccgaaccg tattcgttcg ttgattggtg cgtttgcggg
    12001 cagcaacccg gcagcgttcc atgccgaaga tggcagcggt taccagttcc tggtggaaat
    12061 gcttaccgac ctcaacagcc gtaacccgca ggtggcttca cgcctgattg agccgctgat
    12121 tcgcctgaaa cgttacgatg ccaaacgtca ggagaaaatg cgcgcggcgc tggagcagtt
    12181 gaaagggctg gaaaatctct ctggcgatct gtacgagaag ataactaaag cactggcttg
    12241 atacttttga gccaaaatgc cggatgcgac gcttgacgcg tcttatcagg cctacaagtc
    12301 cgttgaaaat gctggttttg taggctggat aaggcgttca cgccgcatcc ggcagccgtg
    12361 cccaaatgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaatccg ttgaaactgc
    12421 tggttttgtg tgccacatcc ggcatattaa ccatgacgcc gaatcggttg ttcagagtgc
    12481 ccacgctgca tcacccgttg caaaacttcc gcttccagct ccgctaatcg caccgaccct
    12541 aaccggcgcg gacgggggat atccaccgtc aaatccagac caatttttcc ctcttcaatt
    12601 aacaataccc ggtcagccat tgctaccgct tcgctcacat catgcgtcac caacagtacg
    12661 gtaaagccgt gctcctgcca aagtgacaca atcaaatcct gcatctcgag ccgagtcagg
    12721 gcgtccagcg ccccgagcgg ttcatcaagc aataataaac ccggtcgatg aattaacgct
    12781 cttgccagcg ccacacgctg tttctgcccg ccagaaagtg cggcaggcca ttcccctgcg
    12841 cggttctcca gtcctactgc agccaacgcc cgacgcgcgg catcgcgcca ctggccttta
    12901 aggcctaacc caacgttatc aatcaccgat ttccacggta gcagacgcgc atcctgaaac
    12961 atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg tgcccgctaa cacgtcgcct
    13021 gcggttggcg tttccagacc tgccagcagg cgcagcaggg tacttttgcc accaccgctg
    13081 cggcccacca ccgccacaaa ctgacctgcc ggaatatgta aatccagttg gttcaggacg
    13141 atattttccg cgtattgttt gcttactgca ttgagcaaca atggtgtgcc ctggttcaga
    13201 cgagcagtat tcataccgtg gcctccttca aatgataagc cgggttccag cgcaaccaga
    13261 gacgctctaa cagctgcgcg ctgacgtcag caagtttgcc gagcagggcg taaagaataa
    13321 tagcgactac caccacgtcc gtttgcagaa actcccgcgc attcatcgcc agataaccaa
    13381 tgccggaatt ggcagaaata gtttcggcaa cgatcagcgt cagccacatc aggcccaaag
    13441 caaaacgcac gccgaccata attgagggca gggcgccagg caggatcaca tggataaaca
    13501 gcggtatgcc ggataatcca tagctacgcg ccatttccac cagaccgcga tcgatattac
    13561 ggatgccgtg ccaggtgttg atataaatgg ggaatagcgt gccgagcgcc accagaaaga
    13621 tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag cgggatcagc gccagatgcg
    13681 gcacgttacg caacatctga atggaggtat ccagcagtcg ttctccccag cgagatagcc
    13741 cgctaatcag tcccaaaatc aaccccagcg atccgccaat tgaaaagcca atgagcgccc
    13801 gccaggagct gatcgccaga tgctgccaca attcgccgct ggcggagagc gtccagaacg
    13861 ccatcaccac tccttccggt gagggcaaaa tacgcgttga cagccagcca actgaggagg
    13921 ccagttgcca caccgccacg atgcccaccg gtaaaaacca gggggcaacg cgcaataacc
    13981 actttttcac tggcgttgcc attatcgtat tccttagctt tgcgcgacgt tacgggggat
    14041 aaaatcattt gccaccgctt cgccttgcgg attcagcggc tgcggctggg gaatttccgg
    14101 gatggcgaca tccagatgcg ggaacagcaa ctcgccaacc cgatacgctt cttccagatg
    14161 cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg gcgtattcgt tgattcgcgc
    14221 agcgaccgtc ggaccatcgc ccaccagtgc cgtcccggca ccgccgcgca ctaagccaac
    14281 gcccgcccat aaattggggc tgatctccag attgtcgcgc ttgccgttat gtagcgccgc
    14341 cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg gcttgtgctt tggcgatagt
    14401 ctcatcatca agatgcgaga ttaaccgctc ggcggcctgc cacgcttcgt cgttggtttc
    14461 acgaacaatc acatgcagac gaataccgaa acgaattttg cgtccatgcg cggcagcttt
    14521 cgcccgcact tgttcgattt tctctttaac cagttccggc ggttcgcccc aggtgaggta
    14581 gagatcaact tgttctgccg ccagctcctg ggcgacatct gacgatccgc caaagtaaag
    14641 cggaggatac ggctgttgaa tcggcgggaa gagcagtttg gctccgcgca catgaatatg
    14701 tttaccgttg aaattgacgg tttcccccag caataagcgc cgccagacct gggtaaattc
    14761 cgccgaggct tcgtagcgct cgctatgatc aaggaacacg ccgtcgcctg ccagctcttg
    14821 tggatcgctg cctgtgacca gattaaacaa cgcacgacca ttcgagagac ggtcaagcgt
    14881 ggcggcctgg cgggcggcaa cggtaggtga ggttacgctg ggacgcaggg cgacaagaaa
    14941 cttcagccgt tgcgtcaccg ggatcatcga cgcggcaacc agccacgcat cttcacacga
    15001 tcgtcctgtt gggatcagca caccggtata acccagacga tccgccgctt gcgcaatttg
    15061 ttgcagataa ccgtgatcaa ccggacgtga accttcttcc gttcccagat agtgcccgtc
    15121 accatgggtc ggtaaaaacc agaacatatt cagactcata attgttttcc ttccagttga
    15181 gtgggctgcc agatgcgctg gcgaatatcg acttttttcg gcaccagacg gttttcataa
    15241 aacagatctg ccgtttgctg ctgtaaggcg gcaacttcag cgctcaacgg tttgatggtg
    15301 gtgggaggac gatgatccag atatgaggca ataaccggtg ccggtaagcc catcgttttt
    15361 gccagtaaag cgatgctttg ttcgcgctgg ctgcgggtta acgcatcggc ctcactaaag
    15421 gttgtcagca caccctgaat aaaagcgccg tttttttctg catacggacg agctgccaga
    15481 taaaacgatc cagtttgatt gagatcggtg ccgtctttca gcacccgcac gccgccctgt
    15541 aataatgcag cggagtagta gggatcccag atagcccagg cgtcaacgtt accttgctgg
    15601 aacgcggcgc gggcatcggc aggcgtcagg taagtgggct gaatatccgt gaatttaagc
    15661 ccggcctggc gaagggctcg cagtaaaaga ttgtgcgaac tggaaccttt ctgaaaggca
    15721 actttgtgac ctttaagatc ggctacggtt ttgatcgggc tgttttctgc caccagaatc
    15781 acttcggctt tgggtttcgg tggctcgacg cccacgtata ccaaatcagc cccggcagcc
    15841 tgggcaaaga ttggcggaat atccccggta ctgccgagat cgatactgcc aacgtttaac
    15901 gcctccagca tctgtgggcc agcggggaac tccacccagg agatttttgt ttgcggatag
    15961 cgtttttcca gtaactggtg gctttttgcc agcaccatac caatactgcc tttttgatag
    16021 cctatacgta acgcctctgg cgaagactcc gcggcaacag caagtgtaga gacgctaagt
    16081 aaacccgcca gcgccagttt aatgatctta cgcatgggca ttacctcgca gagacagaag
    16141 gtcaggaacc tgaacatcgc ggcggtgcaa cgcctgccag aaagtttcta gcgcggtatc
    16201 aagacgagtt tgcagatttg gcgtgaactg gggtttgtgt tggtaatcaa ttacttgcga
    16261 gtcatcggca aacacgccgt gcaggatctc ctgggctttc agcgtactta aaactggctt
    16321 aagggcataa tcgaccgcca gcagatgggc cacggtgccg cctgtcgcca gcggcagcac
    16381 cactttgcct tgcaacgcac gttctggcag caggtcgagc agggttttca gcgcgccgga
    16441 ataggcggct ttatacacag gcgtggcgac aatcagccca tcggcctgtt gcagctgttc
    16501 ggtgaaggtc ttgagtgccg gactatcgaa acgagcataa agcagatctt ccgggacgaa
    16561 gttttgcaaa ttccagtgat aaacctctac atccaggcca tttagttttt cccgcgcata
    16621 ttccagcaag gagctggagc gagaaggaaa gcgaggacta cccgccaggg tgatgacacg
    16681 catactctct ccttataacc aattgttctt gttttgttaa cattgataac aattcggtca
    16741 gtctgacaga gggcgtccgc gagtcgaaat gatttatctg aggaagaaaa gccaaaaaaa
    16801 gcataaaaaa agcgcaataa gaaaacgttt gctatttatt gccgcaggtc aattcccttt
    16861 tggtccgaac tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca
    16921 tgtactaccc cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt
    16981 ttacttttca gcaattacgc cgtattaccg gaacgccgtt tgaagcactg gtgcggcaga
    17041 aagtgcctgc gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg
    17101 cagccggtct tgataaagac ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat
    17161 cgatcgagat cggtaccgtc acgccacgtc cccagccagg taatgacaag ccgcgtctct
    17221 ttcgtctggt agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg
    17281 ataacctcgt agagaacgta aaaaaagccc attatgacgg cgttctgggt attaacatcg
    17341 gcaaaaataa agatacgccg gtggagcagg gtaaagatga ctatctgatt tgtatggaaa
    17401 aaatctatgc ctatgcggga tatatcgcca tcaatatttc atcgccaaat accccaggat
    17461 tacgcacact gcaatatggc gaagcgctgg atgatctctt aaccgcgatt aaaaataagc
    17521 aaaatgattt gcaagcgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg
    17581 atctttctga agaagaattg atccaggttg ccgatagttt agttcgccat aatattgatg
    17641 gcgtgattgc aaccaatacc acgctcgatc gttctctggt tcagggaatg aaaaattgtg
    17701 atcaaaccgg tggcttaagt ggtcgtccgc ttcagttaaa aagcacagaa attattcgcc
    17761 gcttgtcaca ggaattaaac ggtcgcttac cgatcatcgg tgttggcggc attgattcgg
    17821 ttatcgctgc gcgtgaaaag attgctgcgg gggcctcact ggtgcaaatt tattctggtt
    17881 ttatttttaa aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc
    17941 gaattcctct ttataagata atcagggctt tattttaagc cctggttgtt ttatattcat
    18001 cgctgttgct tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcgaca
    18061 atggattgtg ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga
    18121 cgataactgg cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa
    18181 tggtatgcta tttcgctcac gttttgcgcg caaaatgttg actccagatg ccttttctcc
    18241 cgcaggcttt tgcgttgacg acgccgcgct ctatttctca tttgaagaaa aatgccgcga
    18301 ttttaattta tctaaagaac aaaaagccga actggtgctt aatgcgctgg tagcaattcg
    18361 ttatctcaaa ccgcaaatgc cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt
    18421 gcccatgccg ggcgatgcgg cctgcgtttg gttgagcgac actcacgagc aagtcaattt
    18481 gctggtcgtt gaatctggcg aaaatgccgc actatgcctg ctggcacaac cctgcgttgt
    18541 tattgcgggt cgcgcgatgc agctgggcga cgccatcaaa atcatgaacg acaggctgaa
    18601 accgcaggtt aatgttgaca gcttcaacct cgaacaggca gtctaacgcg tcagcttaag
    18661 cgcagtcttc ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt
    18721 tttcagcggc gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc
    18781 gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca acacctgctg
    18841 gttatttcca cgaaatgcct gtccctgcca gtcaatatct acatttgcgt ccggttgttg
    18901 cgtgatgttg acggtatcat cagcggcggc tgcgccgtaa attttagccg gagccgttgc
    18961 cagaacttcc acctcatcgc caacgcgaat tacgccgcta ttacgggcaa ttaaattctg
    19021 accaaaatcg acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg
    19081 ttcgcctgcc ggatgttttt gccctttttc tggactgacg gtggtgaaaa tacagcggct
    19141 acaaggttta accacatcaa acaccacatc accaatgcga atcactttcc agctatcttc
    19201 ttcccaggct gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact
    19261 ggccggacaa cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata
    19321 gccatcagca aatgacaggg gtacagtgtt gtggcgtttc acgcgccgcg tcatttgtgg
    19381 ccccacccag cgcaactgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc
    19441 gtctggtgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt
    19501 ggcgaaatca gcaaaacgaa catatgcgct actgccatct ggtgcggtga gatgcaagcc
    19561 atcatgcaca ggtgaaggag taaaccgtac catctgggga aactggcgag cggtaataaa
    19621 cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatctgc
    19681 cagggcatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt
    19741 taatgtcgcc acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc
    19801 gcttattagc tataatgcgc aacaattttc ttagcgcctg tcccattagg ctattttatt
    19861 tgtcagtttg gcccagggaa gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc
    19921 tccgcgctgt ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag
    19981 agtaaaagtg acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg
    20041 ttaaaaactg aactggaaaa cctgggggcc gttgaatgcc aggtggttca gggtggggtc
    20101 catttcaagg gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg
    20161 cgtattatgt tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt
    20221 gttcaggcga tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc
    20281 agtggtttga atgacaccat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg
    20341 atcgtcgatg ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg
    20401 gatatccgcg ttaacgtctg gctgcataaa gaaaccgcca gtattgccct tgatctcagt
    20461 ggtgatggtt tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa
    20521 accctggcag ccgcgattgt tatgcgatcc ggctggcagc caggaacacc gctgctcgat
    20581 ccgatgtgtg gttccggtac gttgttgatt gaagcggcga tgctggcgac cgatcgcgca
    20641 ccaggcttgc accgtggacg ttggggcttt agcggctggg cacagcatga tgaagccatc
    20701 tggcaggaag tgaaagcaga agcgcaaacc cgcgcccgta aaggcctggc tgagtatagc
    20761 tctcattttt acggttcgga cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc
    20821 cgtcttgcgg ggattggtga actgatcacc tttgaagtga aagatgtcgc gcaactgacc
    20881 aatccgctgc cgaaagggcc gtacggtaca gtgttgagca acccgccata cggtgaacgt
    20941 ctggacagcg aaccggcgct gattgcgctg catagtctgc tgggccgaat catgaaaaac
    21001 cagttcggcg gctggaatct ctctttattc agtgcctcgc cggatctgtt aagttgcctg
    21061 cagttgcgtg cagacaaaca gtacaaagcg aaaaacggtc cattagactg cgtgcagaaa
    21121 aattaccacg ttgccgaaag cacaccagac agcaaaccgg taatggcagc ggaagactac
    21181 gccaaccgtc tgcgtaagaa cctcaaaaaa ttcgagaagt gggcgcgtca ggaaggcatt
    21241 gaatgctacc gcctgtacga cgccgatcta ccggaataca acgttgcagt tgaccgctac
    21301 gccgattggg tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg
    21361 cgtcagcgtc tgttcgatat tatcgctgca accatttcgg tattggggat tgcgccaaac
    21421 aaactggtgc tgaaaacccg tgaacgccag aagggcaaaa atcagtacca gaaactgggc
    21481 gagaagggcg agtttctcga agttaccgaa tataacgctc acttgtgggt gaacctgacg
    21541 gattacctcg ataccggtct gttcctcgat caccgtatcg cccgtcgtat gctcggtcag
    21601 atgagcaaag gcaaagattt cctcaacctg ttctcttata ccggcagcgc caccgtgcac
    21661 gcgggattag gcggtgcacg cagcactacc accgtggata tgtcgcgtac ttatctggag
    21721 tgggcagaac gcaacctgcg tctgaatggc ctaaccgggc gtgcgcatcg cctgattcag
    21781 gccgattgcc tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ttttatcgat
    21841 ccgccaacct tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat
    21901 ctggcgctga tgaaagattt gaaacgtctg ctgcgtgctg gtgggacgat catgttctcg
    21961 aacaacaaac gtggcttccg tatggatctt gacggactgg caaaactggg actgaaagca
    22021 caagaaatta cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac
    22081 tgctggctga ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg
    22141 catggctgtc gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata
    22201 acgaacgtgt ttgtctggtg ggccgcaacg gcgcaggcaa atcgacgtta atgaaaatcc
    22261 tcaaccgtga acaagggctg gatgacggtc gcattattta cgagcaagat ttgattgtag
    22321 ctcgtctgca acaggatccg ccgcgtaacg ttgaggggag tgtttatgat ttcgttgccg
    22381 aaggcattga agaacaagcg gaatacctga aacgctatca cgatatttcg cgcctggtga
    22441 tgaacgaccc gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagttggatc
    22501 accacaacct gtggcagctg gaaaaccgca ttaacgaagt gctggcgcaa ctggggttag
    22561 atcctaacgt tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac
    22621 gcgcgctggt gagcaatccg cgcgtattgc tgctcgacga accgactaac cacctggata
    22681 tcgaaaccat cgactggctg gaaggattcc ttaaaacctt caatgggacg ataattttca
    22741 tctcccacga ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg
    22801 gcaagcttgt gacctatcct gggaattacg accagtacct gctggaaaaa gaagaagccc
    22861 tgcgcgtgga agaactacaa aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt
    22921 ggatccgcca ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga
    22981 aagcaatgcg tcgtgagcgt ggcgaacgtc gcgaagtgat gggtaccgca aagatgcagg
    23041 tggaagaggc cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg
    23101 ttgacggtaa gcaactggtg aaagattttt ctgcccaggt tctacgtggc gacaaaattg
    23161 ccctgattgg tccgaatgga tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc
    23221 ttcaggcgga cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc
    23281 agcaccgtgc ggaactggat cccgataaaa cggtgatgga taaccttgct gaaggtaagc
    23341 aagaggtgat ggttaacggc aagccacgtc atgttttggg ctatttgcag gacttcctgt
    23401 tccatccgaa acgggcgatg acgccggtac gtgcgctttc tggcggtgag cgtaaccgct
    23461 tgctgttggc gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca
    23521 acgatcttga tgttgaaacg ctggaactgc tggaagaact gatcgacagc taccagggca
    23581 cggtattgct ggtgagccac gatcgtcagt ttgtcgataa caccgttaca gaatgctgga
    23641 tcttcgaagg cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc
    23701 agcaagaaca gtatgtggcg ctcaaacagc ctgcggtgaa aaaaatcgaa gaagccgccg
    23761 cgccaaaagc agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg
    23821 aactggagca gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga
    23881 cgcaggtggc ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa aaagtgctcg
    23941 ccgatatggc tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg
    24001 aagcgttaaa aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa
    24061 cggtaaaatt gcctgatgaa ctgggtttat caggcatgaa aaccaacact ttgttagtca
    24121 tctgtaaaat agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc
    24181 acatcctgtg ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga
    24241 aagcggcatg tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc
    24301 gtcccaccgc ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt
    24361 ttgtgaatat gaacgttgcg ggagtcacca gtgaaattac attactggaa attcccggcg
    24421 tgcttttttc tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg
    24481 ttcccgcgtt ttgtctgata accattctgt tactggtgaa tcgcgcagaa ttaccggtcc
    24541 gtttaaaaga gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga
    24601 ttttcctcgc gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattggcg
    24661 taggcagcag ctttctcccc tggtgtttat tttgtgtcct gcaactgcgt gcttttcagt
    24721 gcgttgatcg tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccagccgc
    24781 taaaacctgg cgtcacgggg atacgccagg ggttacgttc gtgctcctgt tgtacagcaa
    24841 ttcttccagc tgatgatccg gtgtgtccgc gctgtggtac taaagggtac gttcgtcgta
    24901 gaaacagcct gcaatggaca ctcgcgctgc ttgttacgtc catcatgctg tatcttccgg
    24961 ctaatatttt gcccatcatg gtgacggatt tattaggctc gaagatgcca tcgacgattc
    25021 tcgctggggt catcctgtta tggagcgaag gatcttatcc cgtcgctgcg gttatctttc
    25081 tggccagtat tatggtgcca acgttaaaga tgatcgccat agcgtggttg tgttgggatg
    25141 ccaaagggca tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt
    25201 ttgtaggccg ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc
    25261 gtatgggagg tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg
    25321 tcataatgac aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac
    25381 ctgaatcaga gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc
    25441 cagaaagtga agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg
    25501 gcctgggttc ttttttatca ttacagccat caggggccgg aagtgaccct gatcaccgcg
    25561 aatgcggaag gaatagaagg tggtaaaacc accattaaaa gccgtagcgt tgacgtcggc
    25621 gtggttgaaa gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg
    25681 aattccggta tggaaaaatt gctgcataaa gataccgtct tttgggtggt gaaaccgcag
    25741 attggtcgag aagggattag cggcctggga acgctgctgt ctggtgttta tatcgaactg
    25801 cagccaggcg cgaaaggcag caaaatggat aaatacgatt tgctggactc accaccgttg
    25861 gccccgcctg atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc cgggcagctc
    25921 tcgccaggag atcccgtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc
    25981 tttgatacgc aaaaacgcaa tatcagctat caactgttca tcaatgcacc ttatgaccga
    26041 ctggtgacca gcaatgttcg cttctggaaa gatagtggca ttgcggttga tttgacgtca
    26101 gcgggaatgc gtgtggagat gggttcattg acaacgctgc ttagtggcgg tgtcagcttt
    26161 gatgtgccgg aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg
    26221 tatgatgatc agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg
    26281 ttctttaaag attcggtacg cggtctgcaa ccgggagctc cggtagaatt ccggggtatt
    26341 cgcctgggta ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac
    26401 gatgattacc gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt
    26461 ggcgaaaatg cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga
    26521 tcgctgaaaa ccggaaacct ggtcactggc gcactgtatg ttgatctcga tttctatcca
    26581 aatacgcctg caataaccgg tattcgtgaa tttaatggtt atcagattat cccgaccgtt
    26641 agcggcggtc tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa
    26701 ctgccattga atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca
    26761 atgaaaaacc tgcaaacgac gctggatagc atgaacaaga tcctcgccag ccagtcgatg
    26821 cagcagctgc cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc
    26881 ttccagcctg gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag
    26941 gtgttgcgag aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta
    27001 tttgaagcga aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc
    27061 tagtgacgat tgcggcactg tggctggccg gatgcagctc cggcgaaatt aataaaaact
    27121 attaccagtt acctgtggtg cagagcggta cacaaagcac cgccagccag ggtaatcgcc
    27181 tgttatgggt tgagcaggtc gctgttcctg actatctggc ggggaatggt gtggtttatc
    27241 aaaccagtga tgtgaagtat gtgattgcca acaacaacct gtgggccagc ccgttggatc
    27301 aacagttgcg caacactctg gttgccaacc tgagcacgca actgcccggc tgggtggttg
    27361 cctcccagcc tctgggaagc gcccaggata cgctcaatgt taccgtaacg gagtttaacg
    27421 ggcgatatga tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac
    27481 tgatcaaacg tccgttccgt ctggaagggg tgcaaactca ggatggttac gatgaaatgg
    27541 ttaaagtgct ggccagtgtc tggagtcagg aagccgcttc tattgcgcaa gagataaagc
    27601 gtctaccgta atcataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg
    27661 tttttttgtt tctaaggtgc ggaaaaagtt gttcctcttc acattttttg tacaactgac
    27721 atgcccgtgt agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat
    27781 gattcgcggt attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc
    27841 agaaaccagt atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg
    27901 cacatcaacg tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc
    27961 agacgctgaa tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg
    28021 tagtaatggc ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct
    28081 tttgatactc tgtctgatta taatcagaag gcagacgtat cctggaacag accgactttc
    28141 aggtcgctgg cggtatagat cagacgacca tcaaccagca cttcgccatc tgccagaccc
    28201 ataatcagac gtcgattaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct
    28261 gtcggcagta cctgaccggt gaatttcact tcgccaacgc ccagcgcgcg gcctttacct
    28321 tcgccgccca gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg
    28381 catcccggca taaccggatc gccaatgaag tggcatccga agaaccacag atccggattg
    28441 atatccagtt ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg
    28501 accacacggt ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac
    28561 agttcaccgc gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc
    28621 atgttctctg taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga
    28681 acaagtccga tcagttcgga ataaaccagt tcagccaacg caatggccat ggaaaacggt
    28741 gacgtccttc ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg
    28801 tttggccttc gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt
    28861 ctactgccca gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac
    28921 ttaaatggcg aatgttagca gtggggataa tgacaccttg tttcccggtt aactcacgtt
    28981 gctggcaaat agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctgggcac
    29041 gaccgaactg atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga
    29101 gggcgcttat cagggcgcag agttcagcca tcgaggcact atcgccatcc acttcactgt
    29161 atgactgctc aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg
    29221 acatcaggaa cgcctgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg
    29281 ctttgcgttc gatgtcggtg aactcaccat cgccaatatg cacaacgcag ctaatgcgag
    29341 aaggttcgcc aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt
    29401 gcccgatgcg ttcgccttcg gtttcaatca ggatttgctc ctgaagaatc tcatcctgca
    29461 tgcgttcagc gaggaaacct tcacgccatt cacgctgctg cagcattaag tttagctgct
    29521 cgccggagaa ggtgtcgcca tcgcacaggg aggcgacctc tttacactgg cggaggatcc
    29581 actgcgggct aagcggaagt gtttcttgtt caccggtgta gcgggctgct tcgcggataa
    29641 gtaccggcca ggcatccgct cccggtgcag gtaagtgatt atgtcttgcg gtcaatgtta
    29701 cccagcgaca ccactggctt actgactccg catcgacaat ctgcagagta tcttcaaatt
    29761 cgctataaat agcctgctct gaaagctctg gttccatctc ctggaaatca gccaatgatt
    29821 cgcgttcgcc taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga
    29881 gagggcgcga ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca
    29941 gccgcatcca cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc
    30001 catttgcctg atgcaccaga cctggctgca gggtaatgtc gccattaaac tggcgcaggc
    30061 agccaaagag ttgttccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa
    30121 aattatcatc tgcactcact gcgttacgta agcggatggt gtgcccggaa acctcatagt
    30181 gaccgcccac cagttggcct gcatcgcttt gtagcgcacg cgcggcatcg gcaataagat
    30241 tcagatactc agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat
    30301 gcagcaactg ctccagcgca aattgcagtc gcggttgagt atcactgaat aaaggatcgt
    30361 tttcgtcaat caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca
    30421 ggtcacgcca tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag
    30481 ccgcgattat accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg
    30541 catgggcgtt aaagctcaca ggatggagat tcttttcttc actggcccaa aaagctgata
    30601 ttctgtaaag agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat
    30661 cttgaaagcg gttggaaatg gaagtatctg gtgaagaagc accgcgaagg ggagttaatc
    30721 acccgttaca tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa
    30781 aatgaacccg tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat
    30841 cgcatgaagc aaaccattcg ggccagacgg aagcgccatt ttaatgcgga acatcagcat
    30901 acgcgcaaaa aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgcg
    30961 cagcgtcgcg gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac
    31021 aaagagaaat acgcgaataa aatgtcttct ttgaagcagg atctgcaggc attgctgggt
    31081 aaggaataac tgacgaaagt cagttcaatt ttctaaagac gaaaaaaaac cccgcagcag
    31141 cggggttttt taccagacga gaacttaagc ctgcggctga gttacaacgt ctttgatgcc
    31201 tttaacttcg atctctacgc gacgatccgg agccaggcaa tcgatcagtg cagcacgctg
    31261 tttcacgttg tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga
    31321 gattttgtct gccgggatac ctttagagat caggtaatca acaacggact gagcacgacg
    31381 ctcggacaga gcctggttat aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac
    31441 tacggaaccg tctttcggat ccaggttgct cagctggctg tacagctgat ccagagcagc
    31501 ctgaccttcc ggtttcaggg ttgctttgtt gaaggtgaac agaacgtcag acttcagagt
    31561 gaagtgcttg gtctgtactt ccggtgccgg agctggagcc ggagcaacta ctggagctgc
    31621 ttcgccctga ccgaaacggt aggaaacacc caggctcagc atgccgttgt ccggacgagt
    31681 gccgatggtg tgtgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat
    31741 ttcaggagtg atcgcatact caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg
    31801 gtctttagtg gatgcgccac caggtacgtt agacttggtg tctgcacgcc ataccatacc
    31861 acccagacga gtgtaaacgt ccagatcgtc agtgattggg taacccagtt tagcggtcag
    31921 ctgaacgccc tgagctttgt atgcgccgtt gatgttgtcg cctttgtacg gcatacgacc
    31981 taaccagtcg taacccattt caaagccaac atacgggtta acctggtaac caccaaaagc
    32041 acctgcaccc agttggtttt cgtgggtcgg accattgtta ggaataaaac cagtgtcatg
    32101 gtactgggac cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg
    32161 cgctacggta gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt
    32221 ttgcgcctcg ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa
    32281 atccttcacc ggggatctgc tcaatattaa ctctaccgat atcttcggct tatgccgagc
    32341 acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga actccgtcag
    32401 gcatatgaaa aaaaagtctt gtataaggta cgtttaatct tttttgtcag cgacaattta
    32461 cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt agtcacgact
    32521 gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat
    32581 acaaattaga gtgaattttt agcccggaaa gttgtctcgt tgcgtgagag gatgcgctta
    32641 ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat
    32701 gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacgctgtaa ttgcccgtgc
    32761 gtaaagcgcg aaccattgac tcaacggtgt ggcaagggga gagctggcta atctgcatta
    32821 ctttcgttaa gggtagccca gatgcctgaa cccattcgcg actcagtttt tgttgcggtg
    32881 ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa
    32941 gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag
    33001 tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagcacgat
    33061 gtgcatagcc tgaagtgtac ataatcaatc cagccctgtg ggttactgta tgaatataca
    33121 gtacatccgg tgacgacaaa gatcaaccct attttcggaa agagcctcgc aaattttgtc
    33181 gttagtgacg ggaaaacata aattaatctt gccccttaag aataagttgc ctattttcgt
    33241 agttaacgga tccgttaatg tgaatcattc ttttatgtta tgattttaaa aggaatttta
    33301 tgaaaagcct ctcctataag cggatctata aatcacaaga atacctggca acgttgggca
    33361 caattgaata ccgatcattg tttggcagtt acagcctgac cgttgacgac acggtgtttg
    33421 cgatggtttc tgatggtgag ttgtatcttc gggcttgtga gcaaagtgca cagtactgtg
    33481 taaaacatcc gcctgtctgg ctgacatata aaaagtgtgg ccgatccgtt accctcaatt
    33541 actatcgggt tgatgaaagt ctatggcgaa atcaactgaa gctggtgcgt ctgtcgaaat
    33601 attctctcga tgctgcgctg aaagagaaaa gcacgcgcaa tacccgggaa agactgaaag
    33661 atttgcccaa tatgtctttt catctggaag cgattctcgg ggaggtgggg attaaggatg
    33721 tacgggcgtt acgtatactt ggggcaaaaa tgtgttggtt gcgactgcgg cagcaaaaca
    33781 gtctggtgac agaaaagatt ctgtttatgc ttgaaggggc cattatcggc attcatgaag
    33841 ctgcgctccc ggtggcacgc cgccaggagc ttgcagaatg ggctgactct cttacgccga
    33901 aacaggagtt tcctgcggaa cttgagtaat ctggcgttgc agacgaccaa tctctggcag
    33961 taatgcaatc aataacccaa cttgttgtac gaccagaggt tctttgctgt ctgcccgtgg
    34021 ttcaagttgc tgcatccgct gtttcaggcc agctaatgcc tgattgacgc gttcttcatc
    34081 agcaggttga tgatgtaacg cgtcatcaac atagcaaact gcgtcatcaa gaaacgccag
    34141 aatttcagga ttagttaact gctcccggtg agcaccgagg gctgagatat agctggtaaa
    34201 cgtatggtta aggcacagca accgaaacgc ggcttcgcga atttgcgggg taacgttcgg
    34261 ttcgctggac atatttgata ccaccgacgc cagttcagca tcacggttgt gtgcatcgcg
    34321 gcgggcaata cgatacgcca gacggttatc acgcccctga tggtattgct ccagtatggc
    34381 atcgagatac cggcagttgg cctcggtggc gcgttcgagc attcgcggca gattgcgaaa
    34441 cttccagtca ggccagatgt agctcactgc cgcccacgca atggcacaac caatcagcgt
    34501 atcgattacg cgaggtaacg ccacttcaaa accttcaccc agcaagttga aacacagtag
    34561 caccaaaagt gtgatgaaca tcgttgcatg ggcgtattgc acgttacgga aggcaaaaaa
    34621 gagcacgccg gtaataacca gcagcaccag ctgcccttcc agtgatggca caaaccacag
    34681 cacaggaata ccaatcgcga tacctaccag cgtaccaata atccttaact tcaggcggtg
    34741 gcgcgtggcg ttatagtttg gctggcagac aaacaaactt gtcagcaaga tccaataccc
    34801 gtgatgcatt ccggttatct gaatgatggc gtagccgaag cacaacacca gcgacattct
    34861 taccgcatga cggaagaggg cggattccgg cgtgaagtga cggctaagac gcagccagat
    34921 atcactcaat ccgtgcgggc tgtcatcagc gagctcattt tcgtcattat tatggggcag
    34981 tgcctgggcc tgttctgatt caattgttgc cagttgggca tcaatggcac gtaaattgtt
    35041 cagtaaaaat cctagtgttt tgagtaaatc agcaggagcg ccgttatcgc gcatccgctc
    35101 cagcgcagca tcaatatgcg taaaagcgcg ctcaaaatgc ggatcatgtt gataaggctg
    35161 acgcagcaaa atacagcgtg acagttgctg gcacgcctgg ccctgcatcg acatcagccg
    35221 ctgaaaacgg aacagcacgt cgctgtggcg aaaatgttca cgcaatgttt gatattgaat
    35281 atgagaagag ctggcacgct cgtgaatatc ctgcgcgaca aagtaataat gcagtgtgcg
    35341 acgcgttcct cgttgaccac gatcgccacg taagcgggtc agcagcgaga gtttcgtctg
    35401 attcaatgtc gccatcagct gaccgttggc gagagccaaa tcgtacagcg gtgcctggct
    35461 ttcatcttca atatcaggat caaacatgcg cgacttgagc tcaagataac gcgccagttg
    35521 ttcatagcaa cgcgccaggt tgtcctgcag cgggcggacc gggaacagca gatgaccaat
    35581 aagtgtcagg acgttgtacc agacggcacc ggccagcaga tacatcggct gctgatacca
    35641 gtgctcatac agtgacgtcc ccaacatagt gtaaatggcg atcagtaacg caccgaaggc
    35701 aattgttgca tagcgttgac ccagaccgcc gagcaaaatg aagccgctgg tagagagcgt
    35761 taaaccaatt gcaaatagcc agggccaggg aaacagcaat tctactgagg ccgaggcgat
    35821 aaaaaagcag aacagcgtaa tgatgaggtt acgcaaacgt cccgccagtc ggtcatcgag
    35881 atcggtcagc gccgctgcca ccatccccag cgttagcgga atcgtcagtt ttacatcacc
    35941 cagccaccac ggaaacgctg tggttccaca aagcgcaata aaaatacgcg cgtaatacag
    36001 ccaggcgctg ttccaggtat agcgtttgag caaaggactt agcataaagg ccatattagt
    36061 ggattattca aaacgacgac gtgcattggc ttcacgcgca gcttgcgctg tttctaccga
    36121 taccacgcga cgaccgaccg gccatagtgc aatagcggca attttaaagt tcgcaatgcc
    36181 gacaggaatg ccaatgatgg aaatacattg tgcgatgccc gttgcaatgt gcatcaggca
    36241 taaccaccag ccaaagaaaa tcagccagaa aatattcaat accgtaccgc cagtatttag
    36301 cagcacattt ttgccagccg ggttcagttc atcgacatga atagcttcat tgccataagg
    36361 caccagagac agtttagtga tctcccagca ggatcgtgtc agcggtaagg taaaaatcag
    36421 cacaatactg accagagtcg ccaacagcca gcccagagtg gtggcaaatc cgccaagcac
    36481 aaagttcaga atgttcaaaa cggtacgcat aaaacctcgc tttactgtgg ttttcagtaa
    36541 tgggcggcaa ttgtaacgtt tttttgggct ggagcacgtt ttctctgacg gttacactga
    36601 taagaaataa tttcgtgtgg atctacagag tcatggaact gaaagcgaca acgcttggaa
    36661 aacgtctggc acagcaccct tacgatcggg cggtgatcct caatgccggg attaaagtct
    36721 ccggcgatcg ccacgaatac cttattcctt tcaatcaatt actggcgatt cactgtaagc
    36781 gcggtctggt atggggcgag ctggaatttg tactgccggg cgaaaaagtg gtgcgtctgc
    36841 acggtaccga atggggcgag acgcagcgtt tttaccatca tcttgatgct cactggcagc
    36901 ggtggagtag cgagatgagc gaaattgcat ctggtgtttt acgccagcaa ctggatttga
    36961 ttgccacgcg tactggagaa aataaatggc tgacgcgtga gcaaacctct ggcgttcagc
    37021 aacaaatccg ccaggctttg tcggcgttgc cgttgccggt taaccgactg gaagaattcg
    37081 ataactgccg tgaggcatgg cgtaaatgtc aggcctggtt gaaagatatt gaaagcgctc
    37141 ggttgcagca taaccaggcg tataccgaag ccatgcttac cgagtatgcg gattttttcc
    37201 gccaggtcga gtcttcaccg ctgaatccgg cgcaggcccg ggcagtcgtt aatggcgagc
    37261 attctctgtt agtgctggct ggtgcaggaa gcggaaaaac gtcggtgctg gtggcccgtg
    37321 caggctggtt gctggcgcgt ggtgaagcgt cccctgagca aattttattg ctggcgtttg
    37381 gtcgcaaagc cgctgaagag atggacgagc gtattcgaga acgactacat accgaagaca
    37441 ttaccgcacg cacatttcat gcgctggcgc tgcatattat tcagcagggc agcaaaaaag
    37501 ttccgatagt cagcaaactg gaaaatgata ccgctgcccg tcatgaactt tttattgctg
    37561 agtggcgcaa gcaatgcagc gaaaagaaag cgcaggcgaa gggctggcgg caatggctga
    37621 cggaagaaat gcagtggtca gtgccagaag gtaatttctg ggatgatgaa aaattacagc
    37681 gtcgtcttgc ctctcgcctc gatcgctggg taagtctgat gcggatgcac ggtggtgcac
    37741 aggcagaaat gattgccagt gcacccgaag tgattcgcga tctgttcagt aaacgtatca
    37801 agttgatggc cccgttatta aaagcctgga aaggtgcgct gaaggcagaa aacgctgtcg
    37861 atttttcggg ccttattcac caggcgattg tgattctgga gaaaggtcgc tttatcagcc
    37921 cgtggaagca tattctggtt gatgaatttc aggatatctc gccgcagcgc gcagcgttgt
    37981 tagcggcatt acgcaagcaa aacagtcaga cgacgttgtt cgccgttggt gatgactggc
    38041 aggcgattta tcgcttcagc ggtgcgcaaa tgtcgctcac caccgctttc catgaaaact
    38101 ttggtgaagg tgatcgctgc gatttagaca cgacttaccg ttttaacagt cgtatcggtg
    38161 aggtggcaaa ccggtttatt cagcagaacc caggccagct gaaaaagccg ctaaacagct
    38221 taaccaatgg agacaaaaaa gccgtcacgt tattggatga gagccatctt gacgctttgc
    38281 tggataagct ctctggttat gccaaaccgg aagagcgcat tctgatcctg gcgcgttacc
    38341 atcacatgag gcctgccagc ctggaaaaag cggcaacacg ctggccgaag ttgcaaatcg
    38401 actttatgac cattcatgcc agcaaagggc aacaggcgga ttacgtcatc atcgttggct
    38461 tgcaggaggg gagtgatggt tttccggctg cggcgcggga gtcgattatg gaagaggcgc
    38521 tactgccatc ggttgaggat ttcccggacg ctgaagaacg gcggttaatg tacgtggcgc
    38581 tgacccgggc acgccatcgg gtatgggcac tgtttaacaa agagaatccc tctccatttg
    38641 tcgaaatact gaaaaatctt gatgtgccgg tggcgagaaa accgtaagaa acaggtggcg
    38701 tttgccacct gtgcaatatt acttcagacg gtccgcgaga taacgctgat aatcggggat
    38761 cagaatatcg accgcgtcgt tgaaatgcgg cgactggata atgaagtctg ccgttgccac
    38821 gttggtggcg accggaatgt tccataccgt cgccagacgc agcaaggctt tcacgtcagg
    38881 gtcgtgcggc acggcgttta gtgggtccca gaagaaaatc aatacatcaa ttttcccttc
    38941 tgagatcaat gcgccaacct gctggtcacc ccccattggg ccactcaaca tcgcgttgac
    39001 gttcatgccg gttgcacggg aaattaagtt accggtagtg cctgttgcat acagtacgtg
    39061 ttgttccagt aacggttgat gccgttccac ccagctcatc agcatttgtt tgcagtgatc
    39121 gtgtgccacc agcgcaatat gtttccgcgc aggtaaagtg cgtgtcgtca gttccataat
    39181 gtacatccgt agttaacttt cctacagatt actgtaagca cttatcgctg caagataaag
    39241 accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag atggttgttt ttccgcccat
    39301 tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa accaatactt cagccgttgt
    39361 tctgtgagca cctgagactg cggtggaata gcatccagct cggaaacacc cgaaagcagc
    39421 gtactatcat ctgccatcat cgtggcaaat tgcggtagtg aagcgcgttt cgcggatttg
    39481 ttatagcgtt caacctcgac ctcataatca atcgtttttg ccgttgaggt aatggcgaga
    39541 atatccagct ttaccggaat cggcgtcgca tcgccatcca acaattcaag gcgcggcgcg
    39601 gcatcaaaat ggttagcttc ccgctcattc tccagacgag gcaggcgaaa attgacctgg
    39661 ttgatgagct gggtattaaa actgaccacc agcggtgggg agatatagag ccgttcttca
    39721 ctattggaaa gatgaatcgt cttctcaacg cgaaacacta actgatgcgg tccgttatcc
    39781 agttcaatgc tgtccgcacc gcgcagcaga gagctggaaa ccttcttgcc gtcgagcacc
    39841 agaagatcga catcggttga aagccgcagc gtggtggcaa aaacggatac cggcaaacat
    39901 aatgcaatca aggtggtcac gatgccggtt ttcataggta gctcctgtca aaagaccgcc
    39961 cgaataataa tgtgtcaaaa tttttcttta atcatactta ctaacatata gacatatttc
    40021 ccgcttttgc tctcattcat tcgtattagc tgcatggttg gcatgtcggc tttgtcgtac
    40081 actttgttaa acagtctgga ggaaataatg aaagaaaccg atattgcagg cattttaacg
    40141 tccactcaca ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc cagctatcgc
    40201 gtgatgaaat atctactcga ccaggggtat cacgtcattc cggtttcgcc aaaagttgct
    40261 ggcaaaacac tgctgggcca gcaggggtat ggcacgctgg cagaagttcc tgaaaaagtc
    40321 gatatggtcg atgttttccg gaattcagaa gcggcgtggg gcgtagcgca ggaagcgatc
    40381 gccatcggcg cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca agcggcagtg
    40441 ctggctcggg atgccggatt aaatgtagtg atggatcgct gcccggctat tgagatccct
    40501 cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg ggatttgttc
    40561 agggattagt tacgcagacg cggggcctgg agttgtttac ggatggtctg cgccagctca
    40621 tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg ggcttcagcc
    40681 aggtaggtat gaaccggtag gccgttatcg tcctccatca ccacgtgata ccacggagcg
    40741 gcgcgaagct cgtcattcac cgccagctca tcaggcgacg gttcagagag cgaataaacc
    40801 gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac ctgctggccg
    40861 ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt tgatataagg
    40921 gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag atatagccct
    40981 tccggatagg tagcgatcac cggatgatcg gctgcctgac ggaactgctc tataaattgt
    41041 acatcacgac cggcatcaat tgcggcatcc gcgatgattt tctgaaataa atcgctggtc
    41101 atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcag ctgaatcgcc
    41161 agcatgttga tgtctttata accacgacac gcgcccatca actggctttt attctcaaca
    41221 aacttcggcg ggtccatcac gataacgtca aatttttcac cgcgatcgcg ataagtacgc
    41281 agcaatttaa agacatcatc acggacaaac tcagccttgc tcagatccag tttgttcagc
    41341 tcaacgttct gccgtgcaat atccagggct tcctgggagg tatcaacgct gacaacctgg
    41401 ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg tataggagaa acagttaagt
    41461 acgcgtttat tttcgacgta gcggcgggta gccaggcggc tgtcacgctg atccaggtag
    41521 tagcccgttt tgtgtccgtg ctgaatattc accagcagtt tcatgccgtg ttcttcaatc
    41581 ggcagcaggg caggcggcaa ctcgccggtg atggggccct gagtcagctc catcccttct
    41641 tttttacgta ccgcgacgtc gctgcgatcg taaatggcac attccgggta cagcgtttgc
    41701 agggcgctaa ttaatgccgc gcgctgatac tctgcgccag cactcagcag ttgcagcacc
    41761 agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc tccggcgatt
    41821 aaacgatagc tgtcgaggcc atctttttgc gccagccagt cacgccattt ttgtgcttgc
    41881 tgcaaacggc gggtaaaaaa agcaatgtcg atagattcat tcgggtcaaa cgtccagacg
    41941 cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt tccctgatga
    42001 tcaacaatat cgatggtttc accgaggctg gctttacctt ccatgcgggc aacggccccg
    42061 gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc taacactaaa
    42121 cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt gtccggagtt
    42181 ttccatggat ttgcaacgcg tccattaagg ataaggagag cagcaatgtc gaaagtctgc
    42241 ataattgcct gggtttacgg tcgagttcag ggcgttggat ttcgctatac cacgcagtac
    42301 gaagcgaaaa aactgggatt agccgggtac gccaaaaatc ttgacgatgg cagcgtggaa
    42361 gtggttgcct gcggtgaaga agggcaggta gaaaaattaa tgcagtggtt aaagagtggc
    42421 ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc ctcgggggaa
    42481 ttaaccgatt ttcgtatcca ttaaatacat tttaccggtt ttggcaggcc agcaattttg
    42541 gtggcttgct tagccggacc tttcggaaac agtcggtata aatagcggct gtttcctttt
    42601 tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg agaagtattg
    42661 aattccagat agaaattacg cacaaaacgc accacttccc agtgttctgg cgacagcgta
    42721 atcccttcgt tctctgcaat caccactgcc agcggctcac tccactggct gctttctttg
    42781 agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat aattattact
    42841 accttaatca gactggcggc agtgtaacaa aaaacaaagc cccgcataaa gcgaggctat
    42901 gaaagtgtta gcgggtaaga ttaatcgcgg ctggcgaagc ccagaatgct cagcaggctg
    42961 acgaagatgt tgtacagcga aacatacagg ctaaccgtgg cacgaatata gttcgtttca
    43021 ccgccatgaa tgatgttgct ggtttcaaac aagatagcgc cagaggagat cagaatgaag
    43081 accgcgctga tcgccagatg cagagcaggc agttgcagga agatattagc aaccatacca
    43141 atcagcacca ccacaatacc cgccatcagc ataccgccga ggaacgacat atctttgcgg
    43201 gtggtcagca catatgcgga gcagcagaag aacactaacg ccgttccgcc cagtgccata
    43261 gcgattacgt cacccattcc ggcagacaga taggtattca gaattggtcc gaggatataa
    43321 cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt cgccgtttta
    43381 taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc cggagatggc
    43441 agcatcagca ccgtactggc agttgcggta atcgccgaaa aggccagcgt caggctcagc
    43501 agaaaatagg tattacgcag caccttatgg gtgctaagca gcgatgtacg gtcatgtgaa
    43561 gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa ttaagcagca
    43621 taatgataat gcgtaagggc acccagaagt tttacccatc tttacgcatt tgatctggaa
    43681 caggtttaac agcggattat caggttatta agcaatgata taacgctctg agaatttcga
    43741 cagacaaaag aaaaaggggt tagcatttag ctaacccctt atcttatttg gcggaagcgc
    43801 agagattcga actctggaac cctttcgggt cgccggtttt caagaccggt gccttcaacc
    43861 gctcggccac acttccggaa tgacgcgcac tataaacatc ccgatgcggc gtgtaaaccc
    43921 ctaatttgtt tgtttgcctg aaaaacagcc aaaagtgcat tgatagcatg aaataacagc
    43981 agattgatca tttcatcacc atgaattcct tctcttttac tcgtttagca accggctaaa
    44041 catccccact gcccggcaaa aagaaaaata ggtccatttt tatcgctaaa agataaatcc
    44101 acacagtttg tattgttttg tgcaaaagtt tcactacgct ttattaacat tactttctgg
    44161 cgacgtgcgc cagtgcagaa ggatgagctt tcgttttcag catctcacgt gaagcgatgg
    44221 tttgccttgc tacagggacg tcgcttgccg accataagcg cccggtgtcc tgccggtgtc
    44281 gcaaggagaa gagacgtgcg atatgaataa cgaggaaaca ttttaccagg ccatgcggcg
    44341 tcagggcgtt acccggcgca gctttctcaa atattgtagt ctggctgcca cgtcgctggg
    44401 attaggcgcg ggaatggcac caaagattgc ctgggcgctg gagaacaaac cgcgcattcc
    44461 ggtggtatgg atccacggtc tggaatgcac ctgctgtacc gaatctttta tccgctccgc
    44521 tcacccactg gcgaaggacg tcatcctttc cctgatttcc ctcgattatg acgatacttt
    44581 gatggctgcc gccggaaccc aggcggaaga agtctttgaa gacatcatca cgcaatacaa
    44641 tggcaaatat atcctcgcag tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg
    44701 tatcagcagc ggacgaccgt ttattgagaa actcaaacgt gccgctgccg gagccagtgc
    44761 gattatcgcc tggggaacct gcgcgtcctg gggctgcgtg caggccgcgc gacccaatcc
    44821 gacgcaggca acgcctatcg acaaagtcat caccgacaaa cccattatca aagtacctgg
    44881 ctgcccgccg atcccggatg tgatgagcgc catcattact tacatggtga cctttgatcg
    44941 cttgccagat gtcgacaaaa tgggccgtcc actgatgttt tatggtcagc gtatccacga
    45001 taaatgttat cgccgcgctc acttcgacgc cggagagttc gtccagagtt gggatgatga
    45061 cgctgcccgc aaaggttact gcctgtacaa aatgggctgc aaagggccta ccacctataa
    45121 cgcctgttcc tccacacgct ggaacgatgg cgtttccttc ccgatccagt caggtcacgg
    45181 ctgcctgggt tgtgcggaaa atggtttctg ggatcgcggt tcgttctaca gccgcgtggt
    45241 cgatattccg caaatgggta cccattccac cgccgatacc gtcggtttaa ccgcacttgg
    45301 cgtggtggca gcggctgttg gtgtgcacgc agtcgccagc gccgttgacc agcgcagacg
    45361 ccataaccag caacctacag aaaccgaaca tcagccaggc aatgaggata aacaggcatg
    45421 agcactcagt acgaaactca gggatacacc atcaataatg ccggacgccg cctggtggtc
    45481 gacccgatta cgcgcattga aggccacatg cgctgcgaag tgaatattaa cgatcagaat
    45541 gtgatcacca atgccgtctc ctgcggcacc atgtttcgtg ggctggagat catcctgcaa
    45601 gggcgcgacc cgcgcgatgc gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc
    45661 gtacacgctc tggcttcggt ttacgccatc gaagatgcca tcggtattaa agtgccggac
    45721 aacgccaata tcatccgcaa cattatgctg gcaacgctct ggtgccacga tcatctggtg
    45781 cacttctatc agcttgccgg gatggactgg atcgatgtgt tagatgcgct gaaagccgac
    45841 ccgcggaaaa cctccgaact ggcgcaaagc ctctcctcat ggccgaaatc atcccctggc
    45901 tatttcttcg acgtacaaaa ccgcctgaag aaattcgttg aaggcgggca gttggggatc
    45961 ttccgcaatg gctactgggg gcacccgcag tacaaactgc cgccagaagc caacctgatg
    46021 ggctttgccc actatcttga agctctcgat ttccaacgtg aaattgtcaa aatccacgcg
    46081 gtctttggcg gtaaaaaccc gcatccaaac tggattgtcg gcgggatgcc ttgtgccatt
    46141 aacattgacg aaagcggcgc ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag
    46201 tcaatcatta cccgtacggc ggatttcatt aacaacgtga tgatccccga cgccttagcc
    46261 atcggtcagt tcaacaagcc gtggagcgaa atcggcactg gcctttcgga taaatgcgtt
    46321 ctcagttacg gcgcgttccc ggacatcgcc aacgactttg gcgagaaaag tctactgatg
    46381 cctggcggcg cggtgattaa cggcgacttc aacaatgtgc tgccagtgga tttggttgat
    46441 ccgcagcagg tgcaggagtt tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc
    46501 gggcgtcatc cgttcgatgg cattaccgac ccgtggtaca accccggcga tgtcaaaggc
    46561 agcgatacca acattcagca gctgaatgaa caggaacgct actcgtggat caaagcgcca
    46621 cgctggcgcg gtaatgcgat ggaagtgggg ccgctggcac gcacgttaat cgcttatcac
    46681 aaaggcgatg cggcgaccgt tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg
    46741 ctttccggca tccagtcaac attaggccgc attttgtgtc gcgcgcacga agcgcagtgg
    46801 gccgcaggca agttgcaata tttcttcgac aagctgatga ccaatctcaa aaacggcaat
    46861 ctcgccaccg cctccactga gaagtgggaa ccagcaacct ggccgacaga gtgccgtggt
    46921 gtcggcttta ccgaagcgcc gcgcggagcg ttaggccact gggccgccat tcgcgatggc
    46981 aagattgatc tctaccagtg cgtggtgccg accacctgga acgccagccc gcgcgatcct
    47041 aaagggcaaa ttggcgctta tgaagcggcg ctgatgaaca ccaaaatggc gatccccgag
    47101 caaccgctgg agatcctgcg tactctgcac agctttgatc cgtgcctcgc ctgttcaaca
    47161 cacgtgctga gcgacgacgg tagcgagctg atctccgtgc aggtgcgtta acagcgaagg
    47221 agaatcatca tgcaacagaa aagcgacaac gttgtcagcc actatgtctt tgaagcgcca
    47281 gtgcgtatct ggcactggct gacggtgtta tgcatggcgg tgttgatggt caccggttac
    47341 tttatcggca agccgctacc ttccgtcagc ggcgaggcga cgtatctgtt ctatatgggc
    47401 tacatcaggt taattcactt cagcgccggg atggttttta ccgtggtctt gctgatgcgg
    47461 atctactggg cttttgtcgg caatcgctac tcacgcgagc tgtttatcgt gccagtatgg
    47521 cgtaaaagct ggtggcaggg cgtgtggtat gaaatccgct ggtatctgtt tctggcaaaa
    47581 cgtccgagtg ccgatatagg tcataacccc atcgcccagg cggcgatgtt cggctatttc
    47641 ctgatgtcag tttttatgat catcaccggc tttgcgctgt acagcgaaca cagccagtac
    47701 gctatttttg cgccgttccg ttatgtggtg gaatttttct actggacggg cggcaactca
    47761 atggacgttc acagctggca tcggctgggg atgtggctga ttggcgcgtt tgtgatcggt
    47821 catgtctaca tggcgctgcg tgaagatatc atgtccgacg acacggtgat ctccaccatg
    47881 gtcaacggct accgtagcca caaatttggc aaaataagta acaaggagcg ttcatgagcg
    47941 agcaacgcgt ggtggtaatg gggctgggca acctgctgtg ggccgatgaa ggcttcggcg
    48001 tgcgggtggc ggaacggctg tatgcccatt accactggcc cgagtatgtg gagattgtcg
    48061 atggcggtac tcagggactg aacttgctgg ggtatgtcga aagcgccagt cacttgttga
    48121 ttctcgatgc cattgactac gggctggaac caggaacgct gcgaacctat gccggagaac
    48181 gcattccggc ttatctcagc gcgaagaaaa tgagcctgca ccagaacagc ttctccgaag
    48241 tgctggcgct ggcggatata cgtggtcatt tgccagcaca tattgccctc gtcggtctgc
    48301 aacccgcaat gctcgacgac tacggcggta gcctgagcga actggcgcgg gagcaactgc
    48361 ccgctgcgga acaatcggcg ctggcgcagc tggctgcgtg gggaattgtg ccgcaaccgg
    48421 ctaaggaatc gcgctgtctc aattatgact gtctgtcgat ggaaaattac gaaggcgttc
    48481 gcttgcgtca gtacaggatg aggctggagg aacagggatg agcaacgaca caccatttaa
    48541 tgcgttgtgg caacgaatgc tggcgcgcgg ctggacgccg gtcagtgaat gccgtcttga
    48601 cgactggctt acgcaagcgc cagacggcgt ggtgttatta agtagtgacc cgaaacgcac
    48661 gccagaagtc agcgataacc cggtaatgat tggcgaatta ctgcgcgagt ttcccgacta
    48721 tacatggcag gtagcgattg ctgatcttga gcagagcgaa gccatcggtg atcgttttgg
    48781 cgtatttcgt tttcccgcca cactggtgtt taccggtggt agctatcgcg gcgtgctgaa
    48841 tggtattcac ccgtgggcgg aactgataaa cctgatgcgt gggcttgtcg aaccacaaca
    48901 ggagcgtgcc tcatgagcga aacttttttc catctgttgg ggccaggaac gcaaccgaac
    48961 gatgacagtt tcagcatgaa tccactgccg atcacctgtc aggtgaatga tgaaccgagt
    49021 atggcggccc tggagcaatg tgctcacagc ccgcaggtga ttgcgctgtt aaacgagtta
    49081 caacaacaaa taagcgaacg ccagccaccg ttgggcgagg tgctggcagt cgatctgtta
    49141 aatctcaacg ccgacgatcg tcactttatc aatacgcttc tcggggaagg ggaagtgtca
    49201 gtgcgcatac agcaggctga cgacagtgaa agtgaaatac aggaggcgat cttctgcgga
    49261 ttatggcggg tgcgcagacg tcgcggcgac aagttgctgg aggacaaact ggaggctggc
    49321 tgcgcgccgc tggcattgtg gcaggcggca acgcaaaacg tcttgccgac agattcgctg
    49381 ttaccgccgc ccattgatgg cctgatgaat ggcctaccgt tggcgcatga gctactggcg
    49441 catgtacgta accccgacgc gcagccgcac agcattaatc tgacgcaatt acccatcagc
    49501 gaggctgatc ggctttttct ctcacgtctc tgtgggccgg gaaatattca gattcgcacc
    49561 attggctatg gcgagagcta tatcaacgcc acggggttac gccatgtctg gcatttacgc
    49621 tgcacggaca ccttaaaagg cccgttactg gaaagttatg aaatctgccc aataccggaa
    49681 gtggtgctgg cagcgccaga agatttggtc gactctgcgc agcggcttag cgaggtatgc
    49741 cagtggctgg cggaaggtgc accgacgtaa aaagacggta agtatcgctt tcaggcttat
    49801 gaatatcgca atcggcgaat acctctggtc gtagagtttc aggataaaga gggagatcta
    49861 ccattaacgg gttatttttc tctcttcgcc tacaggagtg cgcatgtggg atgtcattga
    49921 tttatcgcgc tggcagtttg ctctgaccgc gctgtatcac tttttatttg taccccttac
    49981 cctggggctg atttttttgc tggctatcat ggaaaccatt tacgtggtca ccggcaaaac
    50041 aatctaccgc gatatgacgc gcttctgggg taagctcttc ggtatcaact ttgctcttgg
    50101 cgtggctacc ggcctgacga tggagtttca gtttggtact aactggtcat tctattccaa
    50161 ctatgtgggc gatatttttg gcgcaccgct ggcgatggaa gcattaatgg ccttcttcct
    50221 cgaatccacc tttgtcgggc tgttcttctt cggctggcag cggctgaata aataccagca
    50281 cctgctggtg acgtggctgg tggcgttcgg ttcaaatctc tctgcattgt ggatattgaa
    50341 tgccaacggt tggatgcaat acccgaccgg tgcgcatttt gatatcgaca ccctgcgtat
    50401 ggagatgacc agtttcagcg agttggtctt taacccggtc agccaggtga aatttgtgca
    50461 cactgtaatg gcgggctacg tgaccggggc catgtttatt atggcgatca gcgcctggta
    50521 tttactgcgc ggacgggagc gcgatgttgc attacgctcg tttgccatcg gttccgtctt
    50581 cggtactctg gcgattatcg gcacgctgca actcggagac agttctgcgt atgaagtcgc
    50641 gcaagtacaa ccggtaaaac tggcggcgat ggaaggggag tggcaaacgg aacctgcacc
    50701 tgcaccgttc catgtggttg cctggccgga acaggatcaa gagcgtaacg cctttgctat
    50761 caaaattccc gcgctgctag ggatcctcgc cactcactca ttagataaac ccgtgccggg
    50821 tctgaagaat ttgatggctg aaacctaccc acgcttgcaa cgcggacgta tggcctggct
    50881 gttaatgcag gaaatatcgc aaggcaatcg tgagccgcat gtgttgcagg catttcggga
    50941 gctggaaggc gaccttggtt acggcatgtt gctctcccgc tatgcgccgg atatgaacca
    51001 tgtcacagct gcacagtacc aggcggcgat gcgtggcgcg atacctcagg ttgcgccggt
    51061 attctggagt ttccgcatca tggtgggctg tggttctctg ctgctactgg tgatgctgat
    51121 tgcgcttgtc cagacgctgc gtggcaaaat tgaccagcat cgctgggtgc tgaaaatggc
    51181 gctctggagt ttgccgctgc cgtggattgc gattgaagcc gggtggttta tgaccgagtt
    51241 tggtcgtcag ccgtgggcga tacaggacat cttaccgaca tactccgcgc actccgcctt
    51301 aaccacagga caactggctt tctcactgat catgatcgta gggctttaca ccctgttctt
    51361 aatcgccgaa gtctacctga tgcagaaata tgcccgtctg gggccgagcg cgatgcagag
    51421 tgaacaaccg acgcagcaac aggggtaaag gagaaaatca tgtttgatta tgaaacattg
    51481 cgcttcatct ggtggctgct gattggcgtg atcctggtgg tctttatgat ctccgacgga
    51541 tttgacatgg ggatcggctg tctgctgccg ctggtggcgc gtaatgatga tgaacgtcgg
    51601 atagtgataa acagcgttgg cgcgcactgg gaaggcaatc aggtctggtt gatcctcgca
    51661 ggtggggcat tatttgccgc ctggcccaga gtgtatgcag cggcgttttc cggcttttat
    51721 gtggcgatga ttctggtgct gtgctcgctg ttcttccgcc cgctggcctt tgattatcgc
    51781 ggaaaaatcg ccgatgcacg ctggcgtaaa atgtgggatg ccggactggt catcggcagt
    51841 ctggtgccgc cggtagtctt cggtattgca ttcggcaact tgctgctcgg cgtgccgttt
    51901 gctttcacac cgcaattacg cgtggaatat ctcggcagct tctggcaact gctgacgcca
    51961 ttccctttat tgtgtggatt gctcagtctt gggatggtga ttttgcaagg tggcgtctgg
    52021 ttacaactga aaactgttgg tgtgattcat ctgcgttcac agctggcgac caaacgcgcc
    52081 gcactactgg tgatgctgtg ctttttgctg gcgggttact ggctgtgggt tggtattgat
    52141 ggctttgtac tgctcgccca ggatgctaac ggtccttcca atccgttaat gaaactggtg
    52201 gcagtgctac ctggtgcctg gatgaataat ttcgtcgaat cgcccgtttt gtggatcttc
    52261 ccgctgctgg ggttcttctg cccattgctg acggtgatgg cgatttatcg tggtcgcccg
    52321 ggttggggat ttttgatggc gtcattgatg caatttggcg tgattttcac ggcaggcatc
    52381 acgctgttcc cctttgtcat gccgtcaagc gtgagtccga tctccagcct gacgttgtgg
    52441 gacagcactt ccagtcagct gacgctgagc attatgttgg taatcgtgct gatatttttg
    52501 cctattgtgt tgctctacac tctctggagc tactacaaaa tgtggggacg catgacgaca
    52561 gaaactctcc gccgtaacga aaacgagctg tactaaggag caggaacaat gtggtattta
    52621 ctttggttcg tcggcatttt gttgatgtgt tcgctctcca cccttgtgtt ggtatggctg
    52681 gacccgcgtc tgaaaagtta acgaacgtaa gcctgatgtg gtgcattagc atcgcatcag
    52741 gcaatcaata atgtcagata tgaaaagcgg aaacatatcg atgaaagcga tcttaatccc
    52801 atttttatct cttctgattc cgttaacccc gcaatctgca ttcgctcaga gtgagccgga
    52861 gctgaagctg gaaagtgtgg tgattgtcag tcgtcatggt gtgcgtgctc caaccaaggc
    52921 cacgcaactg atgcaggatg tcaccccaga cgcatggcca acctggccgg taaaactggg
    52981 ttggctgaca ccgcgcggtg gtgagctaat cgcttatctt ggacattacc aacgccagtg
    53041 tctggtggct gacggattgc tggcgaaaaa gggctgcccg cagcctggtc aggtcgcgat
    53101 tattgctgat gtcgacgagc gtacccgtaa aacaggcgaa gccttcgccg ccgggctggc
    53161 acctgactgt gcaataaccg tacataccca ggcagatacg tccagtcccg atccgttatt
    53221 taatccccta aaaactggcg tttgccaact ggataatgcg aacgtgactg acgcgatcct
    53281 cagcagggca ggagggtcaa ttgctgactt taccgggcat cggcaaacgg cgtttcgcga
    53341 actggaacgg gtgcttaatt tttcgcaatc aaacttgtgc cttaaccgtg agaaacagga
    53401 cgaaagctgt tcattaacgc aggcattacc atcggaactc aaggtgagcg ccgacaatgt
    53461 ctcattaacc ggtgcggtaa gcctcgcatc aatgctgacg gagatatttc tcctgcaaca
    53521 ggcacaagga atgccggagc cggggtgggg aaggatcact gattcacacc agtggaacac
    53581 cttgctaagt ttgcataacg cgcaatttta tttactacaa cgcacgccag aggttgcccg
    53641 cagtcgcgcc accccgttat tggatttgat catggcagcg ttgacgcccc atccaccgca
    53701 aaaacaggcg tatggtgtga cattacccac ttcagtgctg tttattgccg gacacgatac
    53761 taatctggca aatctcggcg gcgcactgga gctcaactgg acgcttccag gtcagccgga
    53821 taacacaccg ccaggtggtg aactggtgtt tgaacgctgg cgtcggctaa gcgataacag
    53881 ccagtggatt caggtttcgc tggtcttcca gactttacag cagatgcgtg ataaaacgcc
    53941 gctgtcatta aatacgccgc ccggagaggt gaaactgacc ctggcaggat gtgaagagcg
    54001 aaatgcgcag ggcatgtgtt cgttggcagg ttttacgcaa atcgtgaatg aggcacgcat
    54061 accggcgtgc agtttgtaat gcataaaaaa gagcattcag ttatctgaat gctctgaggc
    54121 tgatgacaaa cgaagaactg tctaatgcgt agaccggaaa aggcgttcac gccccatccg
    54181 gccaccttac cttcagtttt actctttctc ggagtaacta taaccgtaat agttatagcc
    54241 gtaactgtaa gcggtgctgg cgcgtttaat cacaccattg aggattgcgc ctttaatatt
    54301 aacgccagcc tgttccagac gctgcattga caagctcacc tctttggcgg tgttcaagcc
    54361 aaaacgcgca accagcaggc tggtgccgac agagcgtccc acgaccgcag catcgctcac
    54421 cgccagcatc ggcggcgtat cgacaatcac cagatcgtaa tggtcgttcg cccattccag
    54481 taattgacgc atccgatcgc gcatcagcag ttcagacggg ttaggtggca cctgaccgcg
    54541 agtaatcaca tcaaagcctc ctttgccaaa atgctggatc actttgttga gctcatcttt
    54601 acctgccaga tattccgaca agccatgttc attactcacg gtaaacaggt tatgcgaata
    54661 accacggcgt aagtcggcat caataaataa cactttttga tcggactggg cgatcaccgc
    54721 tgccagagtt gaactgacga acgttttacc actgtctggc gtcgcaccgg tgatcatcag
    54781 aatgttattc tctgtctcca tcatagcgaa gtgcagactg gttcgtagcg cacgtacggc
    54841 ctccacagca gaatccgccg ggttatccac cgccaggaag gggatatttt tagtacgatg
    54901 gcgctgctga ttagaaaata aatttttctt acgcagacgg gtacgtttat ccagccactc
    54961 ggacattggg atagtggcat aaacgctgat gccgtgctct tccagttgtt ccggggcttc
    55021 tacaccacga cgcaacatcg cacgcgccag cacggcaccc accgaaataa acaggccaag
    55081 aataaaacca agcaccacat tcaacgcttt tttcggtttc actggctgcg gctgagtgac
    55141 tgccgggtcg ataatccgca cgttaccaat cgcactggat ttcgaaatac tcaactcctg
    55201 ctggcggtta agtaattgca gatataccgc acggcccgct tctacgtcac gacttaaacg
    55261 caacacttcc tgttgggtgg aaggcattgc cgatacccgc ttattcaggc gtttgcgttc
    55321 ttgctccagt gtctggcgtt tttccagcag cgcacgataa gttgggtgat ctttcttata
    55381 cagctgggag atctctgcct cgcggaaagt cagctcattg agttgattat caacgttcac
    55441 aatctgctca agaacggctt tggcttccag gttaaggtca accgaatcgc gctgttggcg
    55501 ataaacgttg agtttttctt ccgcctggtc cagctcgctg cgcacttctg gcaactggcg
    55561 ctgtaagaat tcaaggcttt gtgaatcctg cgccgcctgg cgagcgatat tctgttgcaa
    55621 atagttgtta gcgatgctgt tcagaatacg agtaatcaac tggggatcat caccggtcat
    55681 ggtaagttcc agcatcccgc tttctttact gcgttcgcta acggtaaagg tttcctgcaa
    55741 cgcgttaatc gcttccagtt cggtacgctg gctcaggaca aactgcgttc ctggtttggc
    55801 cttaatgtcc gcgatagtca gcgcaacgcc gtctttttcc agacgctggc cgaccatacc
    55861 attgacggtg aacccttcac cttccagcgt atagtggccg ttttccccaa ctgtgagtgt
    55921 cagttgctga tcctgaccat tcagttgtgg gatgtgcatc cagctgatcg ccagctcacc
    55981 tggtttctct ttggttaatc tcgcccagcc gcgacccaca atcggaaaat acttctgctc
    56041 aaccatgtcg cgcagattca gttcagcaat ggttttaccg agaatcatgc gcgattgcag
    56101 cagttggatc tccggtgctg actcgggcga tgagttaggg atcatatcgc tcaggccgct
    56161 gagaatggcg ttgccctgtt tctgctcaac ctggaccaga gtatctgcct gataaattgg
    56221 cgtgcttaac agcgagtaag cgacagcgat cagcgtgaac aacgcggtca cgctgacaat
    56281 aaacttacgg tgatcccata actcgccgac cagacgaagc agatcgatct cattttcctg
    56341 agtgctgcct ggtggcgtat tcatattttt agttgtcatc gtattttata aactcaaatt
    56401 accggctgag gcgcttcgcc cattcctgac tggcgcgctc caacataccg tagacatgct
    56461 caaatgcgtc ctgactttta cgatagggat ccgggatctc tttctgttcc agccattgcc
    56521 caaacagcat tgtcttcccg cggacctcgg gagcgattgc cgtaacctga gcaatatgtt
    56581 ccgactccat tgccagaatc agatcgtaat ttcgtgccat ctcagcagtg agcttacgtc
    56641 cggcatgtcc ttcaagagat acgccatgat tagcggcaac atctgccgct gtcgcatctg
    56701 cagggtgctt taccagacca tgaacacctg ccgatttgac ttttacgcct ggcagacgtt
    56761 tacgcagcaa gcgctcgcca atgggcgaac ggcaaatgtt gccggtacaa accaccagga
    56821 ttgagttaaa ttttagttgg gccatgtttg aatccaccgt gaagtctctg tcaggtcatg
    56881 caccccggaa atcgtcggta ccagtagaga aatcacacgg ttccaacgag ccagcggcgc
    56941 agtagtgaca taaacaatgt cgtaaggttc aagctggaat tccgttccga gtatcattgc
    57001 cgatgcgtcc ttcgcattca gctgataaat attggcgatt ttgccattct gcttgttttg
    57061 cgttgcacga ataacaaaaa tccccgtcgc atcagcgaca tcctgattca taccctcagc
    57121 gttacccagc gcttccgcca gggtcatacc gctgcggtcc attttcagcg tgctctgttt
    57181 acccacttca cccatcacga agactttcag atcatcgttg cgcggaataa ataaaatatc
    57241 gcccgggtgc agcagtttgt tctggcgcaa gtcgccacgc tgcatcagcg catagaggtt
    57301 cactttggtc tttacgccat tctgggtgag caccacgttg cgccagtctg catctgcggt
    57361 cagccccccc gcggcgttaa tcgcatccat gatggtcaga ggaatattgg taatcggctg
    57421 ctgacccgat ttgctgactt caccggtgac gtacgctttt tgcgagcgga acgccgcgac
    57481 gctgacatcg acctgcgggc tttcaatgac tgaatccaga cgcgctgtaa tctcgttgcg
    57541 tacctgggta agcgttttcc cggccacttt caggcgacca atataggggt agaaaattgc
    57601 accatcagcg ttaacccagt tgccggtatc actggcgcta cggtattgcc ctgcgggtgt
    57661 ggttagttcc gggtgatccc agaccgtaac catcaacaca tcaccaatgc cgatacgata
    57721 ttcatagttc gcgatttgct gctccagctc cgggttagcc tgcgacataa tcggtttggc
    57781 gcgcagctga tcaatcaatc ctggcgtgac gggatagata ttgaccattt tgtccaggtc
    57841 atactgattg tcaggtagtt caatgacatc cttgttactg gtcgataaat tctgaccagg
    57901 aacaagggta catgctgtca aagacagaac agccaatgtc agtaccgaaa acttaaaaat
    57961 gttctttttc attggtttat atttatcagt cattgtaatg ttatgaatta cgtcaggtta
    58021 ttgtccaacc ggtacttctc gctctgccgt cattggatag agctggtatt tgcgtgacag
    58081 catttgtcca ccatcacgcg tcaatggtgt ccacgaaacc accgcgcggt tgcggttagg
    58141 tccaatggtc atcaagtcga acggaatcga gatataaaaa cctttactaa agccgccttc
    58201 gccgtagtca tcttttgata cgttactgat tgccgcccat acccctactg ccacgccgct
    58261 gtcaaagcgt ttggcgacgt cgatcgttgc acctttatct tttgccagat attgcccaac
    58321 gctaagtttc atcagtacgc cattgagcgt cggcgggttc cagtaagccg tcacgaaacc
    58381 tgttggcgta gaataatcgg tgaagcgcat catgttgtcc cagtcacgct gcttcacgta
    58441 gttaacgtcc acacccagcg cccagctggc atctagcggg cgataaagca gctcggaacc
    58501 gacaccggca tacatcgttt ccagataacc gccatacacc tggccataga aaccgttgcc
    58561 taagtcggca aagtagttcg cttgcaagtt gttgagataa acgtcattgc gaacgtaatc
    58621 acggatatgc gtgcgcacgc ggggcagggt agagtccgcg ggtaatagcg aagacttaaa
    58681 cttgtcgtag ttattgtaaa tattggtgaa aataccgcca tcaagcagca ggtggtcggt
    58741 aaaccagtag cgggcgctgg acatcagccc cagctggaac atatagaaat cttccgggcc
    58801 gcccagcgac tgtgaaagtg ttgggttgaa agagtagcta aagcgatctt cacgaatacg
    58861 gtagccccga ccaaaggcag aaagatcttc agcttcaaca cgttgttgtt gcagtggctc
    58921 tgattgacca ggcgctgtac cggccagttg tttgcgcagg ctggctacgt cggtttcggt
    58981 agtcaccatc gccatatgct cgcgcttttg cgtcacgcta atcttctcaa cgccttgcgg
    59041 caggttattc accagaatcc ggttggcacg atcgaccgct tcgcgagagt cacggtattt
    59101 gtattgctga ccagacatat acagcgtctt atcgcgtagc tgaatttctg gcgcgtcaaa
    59161 gcccgcgtta tacttcaggg cggtaagttg gtttgctacc gtggtgtact gcaatccttc
    59221 agattccggc gcaggttgat atgccggttt tggcgtatcg cgcagggcag ggcgcagatc
    59281 gttgaaattg gtccgtaacg tgaagccaaa catcaacgtg ttaccgcgtt cataactcag
    59341 gttgagatct gcccagctgg cagcgcgata gactgcgccg acgttgaaat ggcttgcctg
    59401 aggcagtttg cccgcaaaat cattctggta attgttgcca tcgtattcga gtttcagacg
    59461 cagaggattc cacggcgttt gatactcaat accgccaaaa atagaggctg gaccacgaaa
    59521 gatatcgcta aagctgatat ctcccgcatc gtgagactct gcgcgatggc aatatttatc
    59581 gcttacccga caaaacgggt tggtaatatt gcccgcatta ccggcgtagc cccaggccat
    59641 cccgagggtg aaatcgaacg ggcccgcttg cttgctggcc accaaatatt caccgtcaaa
    59701 cagacccgta ccagcaatat cacgtttacc aaacgccact tgcggtagcc agtaaccttc
    59761 ttcccacagg cgaagtttaa aatcgaaaga tttgtctttg tatgactgat cgccgctgaa
    59821 atcttcccac tggctatatt tgcgtgtgcg cacatccgta taacgaatgg tgccttccag
    59881 ccatgggaac agcgccacgg aggtggagta gaaacggtat tgatcgttat cccggtagtt
    59941 tacgctgaat tcaccttccg gtgcgatgcg cgcatttggc atctgcaaca atcctgtgcc
    60001 gccaaagtct gattgcgacg aacccagcgg atccggatag gttaatactt cagcatgaca
    60061 ggcactggag acggcaatgg ccaggcagct taaaagataa gagttcttct tcatcagtca
    60121 ggaacacgtt gcgttaaaac agacacaatc tggtcattta aatcagcata tttttcgggc
    60181 agtacatgcg cagaaaagcc taaccaaagc tggctgccag gcggtggttc cacatgtctt
    60241 ttattccaca gggcaacagg ggcgacgaca gcctcaccct caggcgtaat caccataacg
    60301 ttattcttgt ccgcgcctgc aagacgggtg tgatcttgca gataatcggt aacgctacgg
    60361 cctgccagcc agggcaattg cccagcccca gaaaccgcac ctagcagagt aatagtcacc
    60421 ggacgctgta cggtatagag cgtatagtcc ccgaccagcg ggggattgct gttctcatcc
    60481 actcgtacaa aatcagggtc cagtttgacc ggtaagcgac cagtgatatt gagattgagt
    60541 aactgctggc gcacggattt aatcgttgcc accacatcgt cgtcagcttc tgcttcccag
    60601 gaagcaagct gtgccatgac atgttggtag tctttaagcg ctttagcttt cgccgcagag
    60661 tcggtcagca aggcaccagg ccaccagagg cgatcgcgca gttgcggttg tgtcaccaac
    60721 tggacaacat tttccacggg gccaacggaa agtgtctgtt gttcgccagg cagataaata
    60781 gtcaccgttc cttgcgcaaa ggcatggggt gtcattacgt aaagtacgct ggcaatgaaa
    60841 tacgactgta atttattcat ggtgcgggtt taagaaacgt catttctacg ggaataaccc
    60901 ctgcgcctaa catttgccga ctttgacgaa cctggccagt agccgaatcg acccagaatg
    60961 tgttttgcca ctgtttttct ggcagcgtgg aagtcacctc ttcctgccag acattacaca
    61021 gcacatcgcg acccgcaata ttcagcgtct ctaatccagc aaaggaaaaa gtggaactca
    61081 gggtcgcaga gcgaaaatgg ttgtcttcac tccagagaat atcgcgcgtc cagcggctgc
    61141 cgtcttttat tgccagcgcg ttacgcagag ggtcctgccc ggaatttgtc acttccagca
    61201 gattattatt aagtttgacg gtttttaaaa gacgtccatt gtgggtaacc agcatggcgt
    61261 tatcctggga taaccatttg ctttgttctt gttctatata tcccagtacc acaaagattc
    61321 gttgcccttc attaaggcgt aaatacatcg tggaataagg caacgcctgg atctgctgat
    61381 ccgctacggt gatatcctga ttatcgaaaa ggctggcgcg aaatgtatcg accatacttt
    61441 gctggctgtg cgtacatccc gcaagaaata gtgcgaaaat cgataaaata agagggcgca
    61501 cctttaatcc ttgaaaggtt aattaaaaaa atgcaaatct ctaaagcccg gtacgaatat
    61561 cgcaaccggg tatcatttaa ctttaatttt gacgcgttaa agcagtatta gcgcgtagtg
    61621 gacgtggtgg ttgaggtgtt atgagaaccg ccatcgccac tgtcatttgc tgcaactact
    61681 gccaacgtcg ccacaccaac agccgttgcc acaccgacaa ccgtcgccgt gccgacagcg
    61741 ccagcagcca cgccaccagt ggtagcagca gtcgtggttg cacctgtagt ggtcgttgtg
    61801 gtgccagtag tacccgtcgt tgcctcagtc ccgtttgctg cttcaggggc agcaaatgca
    61861 gctggcgtcg tcaacatgaa ggccataagg attgcagaaa gcttgtgttt cataactttt
    61921 cctttattca tcgcatggac aatacgtacg cttgagatca ggattatcta ctttgagtat
    61981 aacatccttg tgaaatctcc ctttcctcat accgccagca ctatatactg cgggtgggaa
    62041 aggcgcgcag agggaaatat aagattgtta actattcccc tctggaaata cgacttgtga
    62101 gcataagata aatcttagca aaatacaaat taaagataag aaaattgtta agaaagctat
    62161 taactattgt tggtaatata gtttcaagtg gtactataac tggctgttaa gtggcattta
    62221 taactattag gtgcagagat attcgcttaa atggatttgt tgctcggtga aaaagtaact
    62281 tgcgttataa tttatggtgt tgactattga gctattccaa taaaaccagc caactttaaa
    62341 caagcaactc atattaagag agataaacat ttgccgctgt tggtcctcgc aggccattta
    62401 cgcggcaaaa ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg
    62461 cggaaatatg gacctggact tctttacgac cgtcggaggg gataatgaat cctttgccgc
    62521 ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa attccttact
    62581 gaaaatactg cgctgcacta tacggggtta ataaaataaa gccagcgata tttaagaccg
    62641 ccggacggct aaaataaaat ttgcttaatc tcaattatca tgcgttaata gctgcgtcgg
    62701 tttgaaagac agacagcata caaagtagtt tactaaagca gttctcatta tcaggcatta
    62761 tccccttctt ttgagtctct ctcctgaaca ctaagtagtt tctgtactaa agccctgttt
    62821 gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg gtttagtaaa
    62881 atggtttaac gcagataaag gttttggctt tatcactcct gatgatggca gcaaagacgt
    62941 tttcgtccat ttcaccgcca tccagagcaa tgaattccgc acgctgaacg aaaatcagaa
    63001 agttgaattt tctattgagc aggggcaacg tggtcccgcg gcagcgaacg ttgttacgct
    63061 ctaaggttgc cgttattact ccacatctcc atttccgctg tccatgttgt catggttcac
    63121 agtaccgcac atcggcattc gatgtgacgg agcgaaatcc tttaggggca aagtgtattt
    63181 tttgtaaatc gacgatgatc acctttgata acgtcgcgct gcaaatacgc actgaccatg
    63241 cgccgctgga tttcacaaaa taatatcagg ctccctcgtg gagccttttt tatatctgcc
    63301 ttatttttct tcaacgctgt atgtatagta agcgataacc tgttgattat tgaatctttc
    63361 ggggagatgg cttataacat ttcttacctg accagggtac cgggaaccaa caccttactg
    63421 gcgtgttgct gtctttcaag accagaagag gttaacagtg aatattgaag agttaaaaaa
    63481 acaagccgaa acggaaatcg ctgactttat cgcgcaaaaa atcgccgagc tgaacaagaa
    63541 tacagggaaa gaagtctctg aaattcgctt caccgcacga gaaaaaatga ccgggcttga
    63601 aagttatgat gtcaaaatca aaataatgtg attttgtgaa catcacccgt gcgaggtgat
    63661 gttccgcttg ttgctaattt agtggccaat cattggcgct tgtggaatta agcgtcggta
    63721 caattcctcc ggcaccgggc tttgccatac tcccgcatac attgcgtaac caatcaccgc
    63781 aaacataatc cccagaatca gtagcgtcat taaccagcca gacaacgcaa aggctttttt
    63841 atttgccgca ggtttttgca gtgaaaaggt caatgttgag gctaccggac atgactctac
    63901 gcaagtcata cagccggtac attccactgt tcgtacctga attaatttat cgaccgggat
    63961 ccgtgatggg caattttttg cgcatttgcc acagtcgata caactttcgg cattgcgacg
    64021 aatcttaaac ggcgataata gcgaaaccac gcccatcagc gcgccatatg ggcaaagata
    64081 acgacaccag gcatggcgaa taaacaggct ggcaatcagc aaaacgatca tgctgattaa
    64141 tgtcgcggtc cccatatgac gaaagaaatt gagcatttta acgtccatca ccacgctgta
    64201 gggcgacaac ataaaatagt gaatcgcctg agcgggcatc aataacgcaa tatagagaaa
    64261 aaaactcaac agcaaatact tcacgccgcg cagaggagta tccagccagc ggggaaggac
    64321 aaattgccga ccaaacagtt tgttaccgag atcgccgatt aattcagaaa gcgtaccaac
    64381 cgggcataac catgaacaaa aggccttttt taataataga ctgatgacga taaaagcgac
    64441 caataacagc atcgcggcgg cgtggacgga cggtaactgg cctgttgcaa ggctatattt
    64501 cagattcatc agaccggcaa tcggtagcca gccttcgata cctcccggtc tggtgacaaa
    64561 tgtcgtacta cctgccgttt cgtaatagcg cacccaatac cagaacgtga tggcaatata
    64621 aatattcatt gccaacagta ataattgcgt cgctttacgc caggtcgtgg cattgcgcca
    64681 gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc gggttcttct
    64741 tttctctgtc atgattttgc cagtccgtta agttgcatac caaatgccac tattctagtt
    64801 gttcttaact ggctgatatt gattcaaatc gcgttcaggt ctttcttatg caaccatgct
    64861 tccagagcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga taatggctgc
    64921 tgctccagtt gtgcgcatag ctggctggcg atacgcattc ccaggctcga gcaactgctt
    64981 tttagctgat gtgcggcacg ctttattttc tcgctatcct gattggcgcg ggcaatgtcg
    65041 atgtcatcga gaagcggcag ggcatgttgt gtaaataata ccagccattc gtggatcttc
    65101 tccgtcccca ttaactgagc atcttcattg agttgcgata catccagcgg ttgatcgtta
    65161 ttgacttgca gttggagata gtgcgccagt aactgaccga gcacttcacg cggcaccggt
    65221 ttagggataa tcccgcgaaa tagcgaactg gtacgctggc gcaacgtttc gtcgatgaca
    65281 tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg ccgggcaagc
    65341 gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg cgacagcaaa tggttcacta
    65401 ttttgcagtg tctctaaagc ctgcgcggca tttcctacgg caacgacctg cgccccactg
    65461 ctgttcaaca tctcaacggt aatccgctgg gttagcgggt tatcttcaat taacagcaaa
    65521 cgtaaaccgt ccagacgcac cgcctgattg actgtttttg gcacgggtgc agtggcaata
    65581 cgtaacagca agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt
    65641 tcgccaccca tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg
    65701 cgtttgccgc taacctgaac aaatggctgg aagatttcag ccagtttcgc gggatcaata
    65761 ccgcagccgc tgtcttccac ttcgaccagc cattgttcgc catcagtgcg actacgcagg
    65821 ataatgtgcc cttcgtcggt gaaacgcagg gcgttgctca acaggttggt aatgacctga
    65881 cgaatacgct gcggatcgcc cattaacgca gtcggtacat cgtcggcaat tgccgttgcc
    65941 aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt gcagggtact ttccagcaac
    66001 gggcgcggtt caaagggttc atcgctgacc gaaacattct tgccgcctgc ttcgatagcc
    66061 gaataatcga gaatatcgtt gaggatggtc agcaacgatt cgccagagtc ggtaattgcc
    66121 cgcaaatcat cacgctgagc gttaagtgcg gggttatctg ccagtagctg tgcggtaccg
    66181 agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgctgatttt
    66241 gcctggttgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac cagttcctgc
    66301 aattcagctg tacgcgcttt gacctgcgcc gccaactgtt cacggtggcg attcagtgca
    66361 tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg tgtccagttc ccgcacgcca
    66421 gcggtttccg ggaaagggga gtcgatatca ccatccagca agcgttgtag cgcctgcgtt
    66481 tgttctgcaa gagggcgcgt gactgagcga taaaccacgc gccagaggat cagaatcagt
    66541 gcgcaaagag aaactattcc cagcaataac aggctgtatt gcccgcgtgc actggctttt
    66601 tccagatgtg ccagcccgtg ctgattacgc agctcaatgg tgtcgaccag ctgactgact
    66661 tcgctactaa actgcgcgaa ttgggcgatg ttattttggg cgagagtttg caggtgattg
    66721 ctgatttcac tgtcctgctg atacagcgcc agcaaatcgc tatattggct aacggtagtt
    66781 aacgttgttg ctacctgagt acgaacaccc ggatcttcaa tgcgtatttg ccgacgttgc
    66841 agaattttca ccgcattatt gagctgcttt tccagcgttg gcgcattttt ctggatctgt
    66901 tccagcccca gattcatcac catttgctgc acccgcaggg cgctaaggcg cagttcattc
    66961 atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact ttctgcggcc
    67021 tgacgctgat gttgttcgat caaatcgtaa atcccggcct gggtcgctcc ggcggaggtt
    67081 gccgcattat tcgcctgacc ttgcgccagg cgtgcgattt catcggcggc ggcgactatc
    67141 tgctgactga gttgctgttg ttgctggcga agttgcaaac gctgccccac cagttcccct
    67201 tgctgacgta acgaacggga gatctcctgc tcctgttgtt caatagcggt ggtgtcaaaa
    67261 tcttgttccc gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt
    67321 cgcccctgcg cctgccacat cttttcgtta tcggcactgg tcaggttctg cgcggcgaaa
    67381 agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg aatcaatgcc
    67441 tgagtgttgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc caccagggta
    67501 ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg ggtcagggtt
    67561 aaattcacgg tcggtgcact ttgggtgaaa aaggttgagt cgcaaagcgg aatgcatcta
    67621 gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattgtt actactttcc
    67681 cttttcatgt tatcggcatt ttcggctgat aacctgttgc gctggcatga tgcacagcat
    67741 tacacggtgc aagcctctac gccgctaaaa gccaaacgcg catggaaact gtgcgcgctt
    67801 tatcccagtt tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct
    67861 cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc
    67921 cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt
    67981 agcacgacgt catttcccga tctgcaaaag caggtagcaa atctgccggt gatcgaactg
    68041 gtaaatgcta ttgatgctcc ccaggtgaaa agccgcgttg gtgtgccctg gtttcagatg
    68101 ggctatcaac ctgggcgata tctggtgcaa tgggcgcacg gtaacccact gaacgtgctg
    68161 ttgatgcccg gacccgataa cgccgggggc agtaaagaga tggccgaggg ttttcgcgca
    68221 gctattgctg gaagcccggt acgtattgtt gatattgcgc ttggcgataa cgatattgaa
    68281 atccagcgta acctgttgca ggagatgctg gagcgccatc cagaaatcga cgtcgttgcc
    68341 ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt
    68401 accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg
    68461 attatggctg ccagcgatca gatggtctgg cagggggaac tggcggttga gcaggccatc
    68521 aggcaattac aggagcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg
    68581 ccgaaaaatg ccgaccgcga acatatccgc cgctcgctgt caccgggggg atttcgtccg
    68641 gtctattttt atcagcacac atcagcggct aagaaataac cttcgccatg ttgcgtcacc
    68701 agtaaatccg cgctgagttt atggcgtaaa cgacgaatta acacatcgac ggtgcgcaag
    68761 tcagggtttt ccacccgacg cgcagagagc atacgcagca gacgttcacg gctaagaatt
    68821 tcgcccggat tcgtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc
    68881 ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc ggcaaagcga
    68941 tagcagttgt cctgtgtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga
    69001 tttttcaccc gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg
    69061 cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtcccgtaac cagaataatc
    69121 cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggcaaa
    69181 ttaatatcca gcagaattaa atccaccggc tgattctgca taatttcccg cagcccggca
    69241 ccgctcgccg taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc
    69301 tgggtaaccg gctcatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca
    69361 ttctgttcat atctgttcat attctgccgt aagccgttca tcctgaccag tgccgctgtt
    69421 catatttact cattaagatc gcttcactaa accataattt tacaggggct attatgcgga
    69481 aactctggaa cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg ctggtcgcta
    69541 ttgggattgt gattggcatt gcgctgattg tattgccaca cgttgggatc aaagtcacca
    69601 gcacaaccga attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa gaatataaac
    69661 agtcggtgca cttccagaac gcctccggcg tgcgagctga atgccatgac tgtcatatcc
    69721 cgccggatat tccaggcatg gtgaagcgca aactggaagc gagcaacgac atttaccaga
    69781 cctttattgc ccactccatt gatacacctg aaaaattcga agccaaacgc gcggaacttg
    69841 ccgagcgtga atgggcgcga atgaaagaaa acaactcggc aacctgtcgc tcctgccata
    69901 actacgatgc gatggatcat gccaagcagc atcctgaagc ggcgcgtcag atgaaggtgg
    69961 cagcgaaaga taatcaatcc tgcatcgact gtcataaagg tattgcccac cagttaccgg
    70021 atatgagtag tggcttccgt aagcagttcg atgagctgcg cgccagtgct aatgatagtg
    70081 gtgacacgct gtactccatt gatattaagc cgatttatgc ggcgaaaggc gataaagaag
    70141 cgtctggttc tctgctgcct gcttcggaag tgaaagtcct taaacgtgac ggcgactggc
    70201 tgcaaattga aatcactggt tggacggaaa gtgcaggacg tcagcgtgta ctcacccaat
    70261 tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag caggtgaaaa
    70321 cgctggagaa aaccaccgtt gccgacacca ataccgagtg gagcaagttg caggctactg
    70381 cgtggatgaa gaaaggcgac atggtgaacg atatcaaacc gatctgggct tatgcggatt
    70441 cgctgtacaa cggcacctgt aaccagtgcc acggcgcacc ggaaatctct cactttgacg
    70501 ctaacggttg gatcggcacg ctcaacggca tgattggctt taccagcctc gataaacgtg
    70561 aagaacgcac cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca ggtaaggctc
    70621 acggcgataa gaaggaagaa aaataatgaa caataacgat ctctttcagg catcacgtcg
    70681 gcgttttctg gcacaactcg gcggcttaac cgtcgccggg atgctggggc cgtcattgtt
    70741 aacgccgcgc cgtgcaactg cggcgcaagc ggcgactgag gctgtcatct cgaaagaggg
    70801 cattcttacc gggtcgcact ggggggctat ccgcgcgacg gtgaaggagg gtcgcttcgt
    70861 ggcagcaaaa ccgttcgaac tggataaata tccgtcgaaa atgatcgccg gattgccgga
    70921 tcatgtacac aacgcggcgc gtattcgtta tccgatggta cgcgtggact ggctacgtaa
    70981 gcgccatctg agcgacacct cccagcgcgg tgataaccgt tttgtgcgcg tgagctggga
    71041 tgaagccctc gacatgttct atgaagaact ggaacgcgta cagaaaactc acgggccgag
    71101 tgccttgctg accgccagtg gttggcaatc gacggggatg ttccataacg cttcggggat
    71161 gctggcgaaa gcgattgcct tgcatggtaa tagcgtgggt acaggcggag attactctac
    71221 cggggctgcg caggtgatcc tgccgcgcgt agtcggttcg atggaagtgt atgaacagca
    71281 aacctcctgg ccgctggtat tgcagaacag caaaaccatt gtgctgtggg gctccgattt
    71341 gctgaaaaac cagcaagcga actggtggtg cccggatcac gatgtttatg aatattacga
    71401 gcagctaaaa gcgaaagtcg ccgccggtga aattgaggtc atcagcatcg atccggttgt
    71461 cacatccacc catgagtatc tggggcgcga gcatgtgaag cacattgcgg ttaacccgca
    71521 aactgacgtg ccgctgcaac tggctctggc gcatacgctg tacagtgaaa acctgtacga
    71581 caaaaacttc ctcgctaact actgtgtagg ttttgagcag ttcctgccgt atctgctggg
    71641 tgagaaagac ggtcagccga aagatgccgc atgggctgaa aaactgacag gtattaatgc
    71701 cgaaaccatt cgtgggctgg cgcggcagat ggcggcgaac agaacgcaga ttattgctgg
    71761 ctggtgcgtg cagcgtatgc agcacggtga acagtgggcg tggatgattg tcgttctggc
    71821 ggcgatgttg gggcaaattg gcctgccagg tggaggtttt ggttttggct ggcactataa
    71881 cggcgcaggc acgccggggc gtaaaggcgt tattctgagt ggtttctccg gctctacgtc
    71941 gattccgcct gttcacgaca acagcgacta caaaggctac agcagcacca ttccgattgc
    72001 ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt
    72061 aaaactaccg ccgctgaaaa tgtgtatttt tgccggaact aacccgttcc atcgccatca
    72121 gcagatcaac cgcattattg aaggctggcg caagctggaa acggttatcg ccatagataa
    72181 ccagtggacc tcaacctgcc gctttgccga tatcgtgctg cctgcgacca cgcagtttga
    72241 gcgtaacgat ctcgaccagt acggcaacca ctccaatcgt ggcattatcg ccatgaaaca
    72301 ggtcgtgccg ccgcagttcg aggcgcgcaa cgacttcgat attttccgcg agctgtgccg
    72361 tcgctttaat cgcgaagaag cctttaccga agggctggac gaaatgggct ggctgaaacg
    72421 catctggcag gaaggtgtac aacagggcaa aggacgcggc gttcatctgc cagcgtttga
    72481 tgacttctgg aataataaag agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca
    72541 ccaggcattc cgcgaagatc cggatctcga accgctgggc acgccgagtg gcctgattga
    72601 gatctactcg aaaaccatcg ctgatatgaa ctacgacgat tgtcaggggc atccgatgtg
    72661 gtttgagaaa atcgaacgct cccacggtgg gcccggctcg cagaagtatc cgttgcatct
    72721 gcaatctgtg catccggatt tccgacttca ctcgcagtta tgtgagtcgg aaacgctgcg
    72781 tcagcaatat acggtagcgg gcaaagagcc agtgttcatt aacccgcagg atgccagcgc
    72841 gcgcggtatt cgtaacggtg atgtggtacg cgtgtttaac gctcgcggtc aggtgttggc
    72901 aggggcagtg gtttctgacc gctatgcacc cggcgtggcg cgaattcatg aaggggcatg
    72961 gtacgatcca gataaaggcg gcgagcctgg tgcgctgtgc aaatacggta accccaacgt
    73021 gttgaccatc gacatcggta cttcgcagtt ggcgcaggcg accagtgcgc ataccacgct
    73081 ggtggaaatt gagaagtaca acggaacagt ggagcaggtg acggcgttta acggccctgt
    73141 tgagatggtg gcgcagtgcg aatatgttcc cgcgtcgcag gtgaaatcat gaccacgctg
    73201 acagcacaac agattgcctg tgtttacgcc tggctggcgc agttgttctc ccgtgaactg
    73261 gacgatgaac aactgacgca aatcgccagt gcgcagatgg ctgaatggtt ttcgttgctg
    73321 aaaagcgaac cgccgctcac tgcggcggtg aatgagctgg aaaaccgtat tgccactcag
    73381 acagtacgtg acgatgctcg tctggaactg gccgctgact tttgcggcct gtttttgatg
    73441 accgacaaac aagctgcgct gccgtatgca tcggcctaca aacaggacga gcaagagatt
    73501 aaacgcttgt tagttgaggc agggatggaa accagcggca atttcaacga accggcagac
    73561 catctggcga tctatctcga gttgttgagt catctgcatt tttcgctggg agaggggacc
    73621 gttcctgcgc gaagaatcga caatctgcgg caaaaaacac tgacggcgct gcgacaatgg
    73681 ttaccagagt ttgctgcgcg ttgccatcag tacgacagtt tcggttttta cgcggcacta
    73741 agccagttat tgctggtgtt agtggaatgc gaccaccaaa acagataacg tcgtttgttg
    73801 gcctgataag acgcgtttag cgtcgcatca ggcattagtg cgcagttgcc ggatgcggcg
    73861 agaacgcctt atccggccta cgggaacagt gctttttgtg ggcctgataa gatgcgttaa
    73921 gcgtcgcatc aggcattatg gcgcagttgc cggatgcggc gagaacgcct tatccggcct
    73981 acgggaacag tgctttttgt gggcctgata agacgcgtta agcgtcgcat caggcaacaa
    74041 actcacgggt gagccacaaa ccgggaaagc cgctggcgca gcaggcggtt ttcctgcttc
    74101 aggtgcgcaa tatcatccat taacgtcagc gccaccgcga tccccggcca gtccagagcc
    74161 agttcatgac gcaggcgtac cgcgcgttgc accacaatgg cggcatggtc gtcaaatacc
    74221 caggttgtct cctggatctc ccgcggttca accaccccca aaccgacaat ttcattcaac
    74281 tcctcttcag agatgccggt atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca
    74341 ttagccatta tgctttcccc caatctttac gtggatcaaa agacgactgg gcgtctgcca
    74401 gttgctgcca cagcgcggca gtgttttcat ccggtttcgg cggcatcacg attttcagta
    74461 ccgcatacag atcgccggtc tgttttttgc tcaccagacc tttgccttta acgcgcaatc
    74521 gttgcccggc ctggctgcca ggcgggatag tcagcaaaat gctttctttc agtgttggaa
    74581 cggtgacttt agtacccagc gccgcttccc acgggctaac cggcaccact atttccagat
    74641 cctggccgac aatatcaaac agcggatgtg gcgcaatgtg aatcaccagc cacaggtcgc
    74701 catttggacc gccgttttcg cccggcgtcc cctggccttt caggcggatg cgttgaccat
    74761 tgccgacgcc cgccgggatc ttcacattca gcgttttcgg gatttcctgt tcgatcatgc
    74821 caaaggcgtt ataaaccggc aggttatagc tgatggtacg cttatgctca gtaagcgttt
    74881 cttcgaggaa aaccgccact tcgatttcaa tatcgtggcc gcgtgtggcg gggcgttgac
    74941 ggctctggcg ggcatgctga ccgaaaattg acgagaagat atcgtcaaaa tcttcggcgt
    75001 taaaactctg accgtcgcca tgatggaact gacggttaaa ttgcggatcg ttgcgatgtt
    75061 gccacatctg atcatactca gcgcgacgtt gttcatcact taacacttcc caggcttcgg
    75121 cgacctcttt gaagcgggct tcggcatccg gttctttgct gacatcagga tggtatttgc
    75181 gggccagtcg acgataggcg gtcttgattg tcttgagatc gtccgtcggt ttcacgccca
    75241 tgatggcgta ataatccttt aattccatag cgttatctcg cgtaaatcaa cacaaattga
    75301 aggaacccct gtaaggtaac tcctataagt gtagggtaat cctcaaaatt tcatatgcca
    75361 acacagaata tgttattgaa atcatcgcgg aggggaggtc gcctcaaggt gggaggaaca
    75421 tattttattt acctccctcc tgaacttttg gctcatgctt acaagttaaa caataaggaa
    75481 aggaatgcac aactcgacta tttaaatcgt tatgcaattt aatgataaag acatgtgcta
    75541 aaaaccattt ctattagtct cacattcttt ttttattggt aatggtaacg ccgctgattt
    75601 tctttatagc tactgatgta atcattagcg gcgtaaacga ggccggggct aatcaggcat
    75661 agtttgcgtc aaaacttgcc tgtttttaaa ggagaatata gaaaaacagg cgttcaacga
    75721 gccattttgc gaacctgttc ccggaaaaat gacatatttc tgtcacactc tttaattatt
    75781 gataacaaaa gaggtgtgag gaatgaacaa aacgctaatc gccgcaactg tggcagggat
    75841 agttttactc gcttcaaatg ctcaggcaca aaccgtaccg gaaggctatc agctacagca
    75901 agtccttatg atgagccgtc ataacttacg tgcgccgctg gcgaacaatg gcagtgtgct
    75961 ggagcagtcg acgccgaata aatggcctga atgggacgtc cccggtgggc agctcaccac
    76021 taaagggggc gtgctcgaag tgtatatggg ccattatatg cgtgaatggc tggcagaaca
    76081 ggggatggtg aaatcagggg aatgcccgcc accggacacc gtttatgcct atgccaatag
    76141 tctgcaacgc accgtcgcga ccgcgcagtt ctttattact ggcgcattcc ctgggtgtga
    76201 tattcctgtt catcaccagg aaaaaatggg cactatggac cccaccttta acccagtgat
    76261 caccgatgat tccgccgcat tcagtgaaca ggcggtggcg gcaatggaga aagagctcag
    76321 caaactccag cttaccgaca gctacaagct actggaacaa atcgttaact ataaagattc
    76381 tcctgcgtgt aaagagaagc aacaatgttc gcttgtagat ggcaaaaata cctttagcgc
    76441 gaagtatcaa caagaaccgg gcgtttccgg gccgctgaaa gtcggcaact cgctggtaga
    76501 tgcgtttact ttgcaatact acgaaggttt tccgatggat caggtggcct ggggagaaat
    76561 caaatctgac cagcagtgga aggtgttgtc gaagctgaaa aacggttacc aggacagcct
    76621 gtttacctca ccggaagtgg cgcgcaatgt tgcgaaaccg ctggtcagtt atatcgacaa
    76681 agccctggtc accgatcgca ccagtgcacc gaaaatcacg gtattagttg ggcacgactc
    76741 caacattgcc tctctgttaa cggcgctgga tttcaaaccg tatcagttgc atgaccagaa
    76801 cgaacgcacg ccgattggcg gcaaaatcgt tttccagcgt tggcatgaca gcaaagccaa
    76861 tcgcgatttg atgaaaattg aatatgtgta ccagagtgcg gaacagttac gtaatgccga
    76921 tgcgttaacc ctgcaggcac ctgcgcagcg cgtgacgctg gaattaagcg gttgcccgat
    76981 agatgccaac ggtttttgcc caatggataa gtttgatcgc gtattgaatg aagcggtgaa
    77041 ataacagaaa actcccccgc gtgaagcggg ggagtcgctg gttaaacgtt tttacgttcg
    77101 atggtctgtt cgccccaaaa aagcgaatct ttatcggtct tagcaaaggc tttgactaac
    77161 acttcatcgc taccttcttc ccaaatcttt tccgccattt tttcgtcgta cccggcgact
    77221 tcgaaaatgg cctcggctat ttccggcgac gtattgcgca gagatgccca ttcaccgacg
    77281 tgatgagctt tcgcttcttg agttggcatg cgtatcctcc tgttgaagat tagctgttaa
    77341 gtttaactgc cagacctgcg acatattccc cttgataacg agcaatagac agttcttcct
    77401 ggctgggctg gcgtgaaccg tcaccgcctg cgatggtggt tgcgccgtac ggcgtaccgc
    77461 cgcgaacctg tgaaacgtca aataattcct gcgctgcgta gccaatgggg acaatcacca
    77521 tgccgtgatg cgcaagggtc gtccaggtgg atgtgatggt ttgttcctga ccgccgccag
    77581 taccggtgga actaaagacg ctcgccagtt ttccgtatag tgcgccggaa gcccacaggc
    77641 cgcccgtctg gtcgaggaag gtacgcattt gaccggacat gttaccaaag cgggtaggtg
    77701 taccaaaaat aatggcgtcg taatcggcca gttcttgcgg ggttgcaacc ggtgcagttt
    77761 gcgttttacc gcctgctttt tcaaataatt gcggcggcat ggtttccggt acacgcttaa
    77821 cgacaacttc tgcgccatcc actttgcttg caccctcagc aactgcgcgt gccatcgttt
    77881 caacatgtct aaggatatgg tttatcattt tgaaaaatat aattttattt catcctcctg
    77941 atcactttgg ggcacgtctg gggcgcggga attaaggata ttattcaaca tagctacctg
    78001 agtcacgctg cactcaggca tccatgcacc ataaacattg tagaccatgc tggcgctgga
    78061 gtgccccatc tgtgacgcaa taaatgtcgg gtttgctccg gcagataaag cccagcacgc
    78121 ataggtatgg cgtgactgat acgctttacg ggatcggata cccgctcttt ttattgctga
    78181 atcccatgtc gctccgatgg agcttaccgc gtagttaata cccgccttgt gattcttgcg
    78241 aacgatttgc ggacagaaaa caaaagtgca ctcgtgcaaa attgttcttc cgtactcgcg
    78301 taatttaaca gtgatctgat gctgcctgcc aagacgagta agcatcgcct ggtttttaag
    78361 tgcttcaatt gctggtgcca gaagatgtat aacccggtta gtgcctgcgt cagtctttgg
    78421 tagcgtaaaa tcacctattt ttgtaaaatt tcttcgcact gttatcgtgc cagctttcag
    78481 gtcgatatcc tcccatgcaa gtgcggcaat ttcaccgtgt cgcatccctg taaaaacagc
    78541 cactgtccag aggtttttgg tctgttgatg atggcaggca tcaatgagac ggctaaactc
    78601 atctctggtc agtggatccg gcactggttt tgatttcctc agcggtgtta ttgaattaaa
    78661 cgggtttttc tccagatacc cgttttcggc ggcaaagctg aacattccgg ctgttgttgt
    78721 catgtaatag ttcactgtgg gtacggttct tcctttttgg gacgtgctgg ttttcctgct
    78781 tcccttttcc ccggtcagta aatctttcct gataaacagc agatcttctt ttgtaatcga
    78841 tgacgccagc ctgccaggac caagtctcgg tagcatattt ttcatcactg attgataacg
    78901 atttaaggcg ttactactga tttccattgc tttcagagta agccatttct gtgccagttc
    78961 accgacggtg atatcttttt ttaccaggcc aaatagcttc aggttaggtg agtcagggaa
    79021 tcgctcggca taatcaaacg ttcccgttct gattgcaaag cagacagaag cccttaactc
    79081 accagcgatc tttctgtttt tcggagtatc gggcacgcga agattttcgc gcactcgttt
    79141 acccctgtac ttaaacgtta ttcggagttt tcctccgtga ttttcaacgc cggctggata
    79201 ggctgaattc gccattgttc ctcctgcgtc caagagcacg ctcaggttat actcttatgg
    79261 acaagaatta aacatcctga gatggtaagg gttggttttt gatccaagtg ttgatggtag
    79321 gaagatgata gagacattcg ctgtttttct ttggcgttcc gtcgggggca atttgtttat
    79381 attctctgcc gttcatccac gcactgtccc tggcgcgtaa tattgttcct tttcttaggc
    79441 ctgttacagc cattaaaagc tccagtgtta cccattcatt cgcatttatc tgtacaacag
    79501 gtgtgatgac tggctgaatg ccatggttat gttggtttac taactcgcgc ataagttgcc
    79561 tctgcatgtg taagaaaaaa ccgcccgaag gcggttgtca gttgattgat gtacggcgca
    79621 tttttcgaag gctggcaata tgcttttcct tctcaatttc cgctttaatc atatgcagtt
    79681 cgttgtgctc gattcgctca aattcttcat taaatgcact aattgaagtg gctctggttc
    79741 tgccgtcgag ccttcggtag atcacctgag tcagagttac cttgcatatt tctaccggat
    79801 agttgttggc atcaacgaaa gactgcccgc gctggatcag gacgaacacg gtttgcactc
    79861 cctggatttc cgctacgttc actctgctaa ttcgttgatc aggtcacgat atttattaag
    79921 ttctcgcaac gctttacatg cctcttccca acgtttctgt ttccgtccgg cgcggcgtgc
    79981 ttctttttgt gatttgcgca gcaggtcacc tacaatgttg
//
LOCUS       DAZGTJ010000011        79318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000011 DAZGTJ010000000
VERSION     DAZGTJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.011"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            104..364
                     /gene="iraP"
                     /locus_tag="ACHK8E_000992"
     CDS             104..364
                     /gene="iraP"
                     /locus_tag="ACHK8E_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP0343294.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            464..1879
                     /gene="phoA"
                     /locus_tag="ACHK8E_000993"
     CDS             464..1879
                     /gene="phoA"
                     /locus_tag="ACHK8E_000993"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP0343295.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            1998..2318
                     /gene="psiF"
                     /locus_tag="ACHK8E_000994"
     CDS             1998..2318
                     /gene="psiF"
                     /locus_tag="ACHK8E_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP0343296.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2420..3535
                     /gene="adrA"
                     /locus_tag="ACHK8E_000995"
                     /gene_synonym="dgcC"
     CDS             2420..3535
                     /gene="adrA"
                     /locus_tag="ACHK8E_000995"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP0343297.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3552..4361)
                     /gene="proC"
                     /locus_tag="ACHK8E_000996"
     CDS             complement(3552..4361)
                     /gene="proC"
                     /locus_tag="ACHK8E_000996"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP0343298.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4481..4939
                     /gene="yaiI"
                     /locus_tag="ACHK8E_000997"
     CDS             4481..4939
                     /gene="yaiI"
                     /locus_tag="ACHK8E_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP0343299.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5122..5646
                     /gene="aroL"
                     /locus_tag="ACHK8E_000998"
     CDS             5122..5646
                     /gene="aroL"
                     /locus_tag="ACHK8E_000998"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP0343300.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5696..5887
                     /gene="yaiA"
                     /locus_tag="ACHK8E_000999"
     CDS             5696..5887
                     /gene="yaiA"
                     /locus_tag="ACHK8E_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP0343301.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6145..6822
                     /gene="aroM"
                     /locus_tag="ACHK8E_001000"
     CDS             6145..6822
                     /gene="aroM"
                     /locus_tag="ACHK8E_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP0343302.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            6894..7178
                     /gene="ppnP"
                     /locus_tag="ACHK8E_001001"
     CDS             6894..7178
                     /gene="ppnP"
                     /locus_tag="ACHK8E_001001"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP0343303.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7335..7664
                     /locus_tag="ACHK8E_001002"
                     /pseudo
     CDS             7335..7664
                     /locus_tag="ACHK8E_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            7661..7741
                     /locus_tag="ACHK8E_001003"
     CDS             7661..7741
                     /locus_tag="ACHK8E_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343304.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(7822..8733)
                     /gene="rdgC"
                     /locus_tag="ACHK8E_001004"
     CDS             complement(7822..8733)
                     /gene="rdgC"
                     /locus_tag="ACHK8E_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0343305.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8858..9766
                     /gene="mak"
                     /locus_tag="ACHK8E_001005"
     CDS             8858..9766
                     /gene="mak"
                     /locus_tag="ACHK8E_001005"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP0343306.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(9905..11089)
                     /gene="araJ"
                     /locus_tag="ACHK8E_001006"
     CDS             complement(9905..11089)
                     /gene="araJ"
                     /locus_tag="ACHK8E_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP0343307.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11215..14358)
                     /gene="sbcC"
                     /locus_tag="ACHK8E_001007"
     CDS             complement(11215..14358)
                     /gene="sbcC"
                     /locus_tag="ACHK8E_001007"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP0343308.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     PLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(14355..15557)
                     /gene="sbcD"
                     /locus_tag="ACHK8E_001008"
     CDS             complement(14355..15557)
                     /gene="sbcD"
                     /locus_tag="ACHK8E_001008"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP0343309.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            15747..16436
                     /gene="phoB"
                     /locus_tag="ACHK8E_001009"
     CDS             15747..16436
                     /gene="phoB"
                     /locus_tag="ACHK8E_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP0343310.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16494..17789
                     /gene="phoR"
                     /locus_tag="ACHK8E_001010"
     CDS             16494..17789
                     /gene="phoR"
                     /locus_tag="ACHK8E_001010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP0343311.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            18196..19515
                     /gene="brnQ"
                     /locus_tag="ACHK8E_001011"
     CDS             18196..19515
                     /gene="brnQ"
                     /locus_tag="ACHK8E_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP0343312.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19591..20964
                     /gene="proY"
                     /locus_tag="ACHK8E_001012"
     CDS             19591..20964
                     /gene="proY"
                     /locus_tag="ACHK8E_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP0343313.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            21120..22937
                     /gene="malZ"
                     /locus_tag="ACHK8E_001013"
     CDS             21120..22937
                     /gene="malZ"
                     /locus_tag="ACHK8E_001013"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP0343314.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(22942..23523)
                     /gene="acpH"
                     /locus_tag="ACHK8E_001014"
     CDS             complement(22942..23523)
                     /gene="acpH"
                     /locus_tag="ACHK8E_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP0343315.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            23616..24686
                     /gene="queA"
                     /locus_tag="ACHK8E_001015"
     CDS             23616..24686
                     /gene="queA"
                     /locus_tag="ACHK8E_001015"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP0343316.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24741..25868
                     /gene="tgt"
                     /locus_tag="ACHK8E_001016"
     CDS             24741..25868
                     /gene="tgt"
                     /locus_tag="ACHK8E_001016"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP0343317.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            25891..26223
                     /gene="yajC"
                     /locus_tag="ACHK8E_001017"
     CDS             25891..26223
                     /gene="yajC"
                     /locus_tag="ACHK8E_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP0343318.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26251..28098
                     /gene="secD"
                     /locus_tag="ACHK8E_001018"
     CDS             26251..28098
                     /gene="secD"
                     /locus_tag="ACHK8E_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP0343319.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28109..29080
                     /gene="secF"
                     /locus_tag="ACHK8E_001019"
     CDS             28109..29080
                     /gene="secF"
                     /locus_tag="ACHK8E_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP0343320.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29210..29557
                     /gene="yajD"
                     /locus_tag="ACHK8E_001020"
     CDS             29210..29557
                     /gene="yajD"
                     /locus_tag="ACHK8E_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP0343321.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29595..30479)
                     /gene="tsx"
                     /locus_tag="ACHK8E_001021"
     CDS             complement(29595..30479)
                     /gene="tsx"
                     /locus_tag="ACHK8E_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0343322.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30778..31317)
                     /gene="yajI"
                     /locus_tag="ACHK8E_001022"
     CDS             complement(30778..31317)
                     /gene="yajI"
                     /locus_tag="ACHK8E_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0343323.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            31468..31917
                     /gene="nrdR"
                     /locus_tag="ACHK8E_001023"
     CDS             31468..31917
                     /gene="nrdR"
                     /locus_tag="ACHK8E_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP0343324.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            31921..33024
                     /gene="ribD"
                     /locus_tag="ACHK8E_001024"
     CDS             31921..33024
                     /gene="ribD"
                     /locus_tag="ACHK8E_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP0343325.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWTETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33113..33583
                     /gene="ribE"
                     /locus_tag="ACHK8E_001025"
     CDS             33113..33583
                     /gene="ribE"
                     /locus_tag="ACHK8E_001025"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP0343326.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33603..34022
                     /gene="nusB"
                     /locus_tag="ACHK8E_001026"
     CDS             33603..34022
                     /gene="nusB"
                     /locus_tag="ACHK8E_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP0343327.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34100..35077
                     /gene="thiL"
                     /locus_tag="ACHK8E_001027"
     CDS             34100..35077
                     /gene="thiL"
                     /locus_tag="ACHK8E_001027"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP0343328.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            35055..35573
                     /gene="pgpA"
                     /locus_tag="ACHK8E_001028"
     CDS             35055..35573
                     /gene="pgpA"
                     /locus_tag="ACHK8E_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP0343329.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(35627..36601)
                     /gene="yajO"
                     /locus_tag="ACHK8E_001029"
     CDS             complement(35627..36601)
                     /gene="yajO"
                     /locus_tag="ACHK8E_001029"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP0343330.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(36656..38518)
                     /gene="dxs"
                     /locus_tag="ACHK8E_001030"
     CDS             complement(36656..38518)
                     /gene="dxs"
                     /locus_tag="ACHK8E_001030"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP0343331.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(38543..39442)
                     /gene="ispA"
                     /locus_tag="ACHK8E_001031"
     CDS             complement(38543..39442)
                     /gene="ispA"
                     /locus_tag="ACHK8E_001031"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP0343332.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(39442..39684)
                     /gene="xseB"
                     /locus_tag="ACHK8E_001032"
     CDS             complement(39442..39684)
                     /gene="xseB"
                     /locus_tag="ACHK8E_001032"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP0343333.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39890..41338
                     /gene="thiI"
                     /locus_tag="ACHK8E_001033"
     CDS             39890..41338
                     /gene="thiI"
                     /locus_tag="ACHK8E_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFP0343334.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41392..41982)
                     /gene="yajL"
                     /locus_tag="ACHK8E_001034"
     CDS             complement(41392..41982)
                     /gene="yajL"
                     /locus_tag="ACHK8E_001034"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP0343335.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(41945..42856)
                     /gene="panE"
                     /locus_tag="ACHK8E_001035"
     CDS             complement(41945..42856)
                     /gene="panE"
                     /locus_tag="ACHK8E_001035"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP0343336.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43024..43515
                     /gene="yajQ"
                     /locus_tag="ACHK8E_001036"
     CDS             43024..43515
                     /gene="yajQ"
                     /locus_tag="ACHK8E_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP0343337.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43643..45007)
                     /gene="yajR"
                     /locus_tag="ACHK8E_001037"
     CDS             complement(43643..45007)
                     /gene="yajR"
                     /locus_tag="ACHK8E_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343338.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(45156..46046)
                     /gene="cyoE"
                     /locus_tag="ACHK8E_001038"
     CDS             complement(45156..46046)
                     /gene="cyoE"
                     /locus_tag="ACHK8E_001038"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP0343339.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(46058..46387)
                     /gene="cyoD"
                     /locus_tag="ACHK8E_001039"
     CDS             complement(46058..46387)
                     /gene="cyoD"
                     /locus_tag="ACHK8E_001039"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP0343340.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(46387..47001)
                     /gene="cyoC"
                     /locus_tag="ACHK8E_001040"
     CDS             complement(46387..47001)
                     /gene="cyoC"
                     /locus_tag="ACHK8E_001040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP0343341.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(46991..48982)
                     /gene="cyoB"
                     /locus_tag="ACHK8E_001041"
     CDS             complement(46991..48982)
                     /gene="cyoB"
                     /locus_tag="ACHK8E_001041"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP0343342.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(49004..49951)
                     /gene="cyoA"
                     /locus_tag="ACHK8E_001042"
     CDS             complement(49004..49951)
                     /gene="cyoA"
                     /locus_tag="ACHK8E_001042"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP0343343.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(50411..51886)
                     /gene="ampG"
                     /locus_tag="ACHK8E_001043"
     CDS             complement(50411..51886)
                     /gene="ampG"
                     /locus_tag="ACHK8E_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP0343344.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(51930..52508)
                     /gene="yajG"
                     /locus_tag="ACHK8E_001044"
     CDS             complement(51930..52508)
                     /gene="yajG"
                     /locus_tag="ACHK8E_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0343345.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            52813..53130
                     /gene="bolA"
                     /locus_tag="ACHK8E_001045"
     CDS             52813..53130
                     /gene="bolA"
                     /locus_tag="ACHK8E_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP0343346.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            53474..54772
                     /gene="tig"
                     /locus_tag="ACHK8E_001046"
     CDS             53474..54772
                     /gene="tig"
                     /locus_tag="ACHK8E_001046"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0343347.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            55018..55641
                     /gene="clpP"
                     /locus_tag="ACHK8E_001047"
     CDS             55018..55641
                     /gene="clpP"
                     /locus_tag="ACHK8E_001047"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP0343348.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            55767..57041
                     /gene="clpX"
                     /locus_tag="ACHK8E_001048"
     CDS             55767..57041
                     /gene="clpX"
                     /locus_tag="ACHK8E_001048"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP0343349.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            57229..59583
                     /gene="lon"
                     /locus_tag="ACHK8E_001049"
     CDS             57229..59583
                     /gene="lon"
                     /locus_tag="ACHK8E_001049"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP0343350.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            59774..60064
                     /gene="hupB"
                     /locus_tag="ACHK8E_001050"
     CDS             59774..60064
                     /gene="hupB"
                     /locus_tag="ACHK8E_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP0343351.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            60256..62127
                     /gene="ppiD"
                     /locus_tag="ACHK8E_001051"
     CDS             60256..62127
                     /gene="ppiD"
                     /locus_tag="ACHK8E_001051"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0343352.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            62278..62649
                     /gene="ybaV"
                     /locus_tag="ACHK8E_001052"
     CDS             62278..62649
                     /gene="ybaV"
                     /locus_tag="ACHK8E_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP0343353.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            62743..63141
                     /gene="fadM"
                     /locus_tag="ACHK8E_001053"
     CDS             62743..63141
                     /gene="fadM"
                     /locus_tag="ACHK8E_001053"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP0343354.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(63193..63888)
                     /gene="queC"
                     /locus_tag="ACHK8E_001054"
     CDS             complement(63193..63888)
                     /gene="queC"
                     /locus_tag="ACHK8E_001054"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP0343355.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(63953..65653)
                     /gene="ybaE"
                     /locus_tag="ACHK8E_001055"
     CDS             complement(63953..65653)
                     /gene="ybaE"
                     /locus_tag="ACHK8E_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP0343356.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65753..66571
                     /gene="cof"
                     /locus_tag="ACHK8E_001056"
     CDS             65753..66571
                     /gene="cof"
                     /locus_tag="ACHK8E_001056"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP0343357.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            66724..67182
                     /gene="decR"
                     /locus_tag="ACHK8E_001057"
     CDS             66724..67182
                     /gene="decR"
                     /locus_tag="ACHK8E_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP0343358.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            67212..68984
                     /gene="mdlA"
                     /locus_tag="ACHK8E_001058"
     CDS             67212..68984
                     /gene="mdlA"
                     /locus_tag="ACHK8E_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0343359.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            68977..70758
                     /gene="mdlB"
                     /locus_tag="ACHK8E_001059"
     CDS             68977..70758
                     /gene="mdlB"
                     /locus_tag="ACHK8E_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0343360.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            70939..71277
                     /gene="glnK"
                     /locus_tag="ACHK8E_001060"
     CDS             70939..71277
                     /gene="glnK"
                     /locus_tag="ACHK8E_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP0343361.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            71307..72593
                     /gene="amtB"
                     /locus_tag="ACHK8E_001061"
     CDS             71307..72593
                     /gene="amtB"
                     /locus_tag="ACHK8E_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP0343362.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(72642..73502)
                     /gene="tesB"
                     /locus_tag="ACHK8E_001062"
     CDS             complement(72642..73502)
                     /gene="tesB"
                     /locus_tag="ACHK8E_001062"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP0343363.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            73720..74289
                     /gene="ybaY"
                     /locus_tag="ACHK8E_001063"
     CDS             73720..74289
                     /gene="ybaY"
                     /locus_tag="ACHK8E_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP0343364.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(74320..74631)
                     /locus_tag="ACHK8E_001064"
     CDS             complement(74320..74631)
                     /locus_tag="ACHK8E_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP0343365.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            74794..74890
                     /gene="ffs"
                     /locus_tag="ACHK8E_001065"
     ncRNA           74794..74890
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK8E_001065"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            75010..75363
                     /gene="ybaA"
                     /locus_tag="ACHK8E_001066"
     CDS             75010..75363
                     /gene="ybaA"
                     /locus_tag="ACHK8E_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP0343366.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(75405..76955)
                     /gene="pdeB"
                     /locus_tag="ACHK8E_001067"
     CDS             complement(75405..76955)
                     /gene="pdeB"
                     /locus_tag="ACHK8E_001067"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP0343367.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(77119..77589)
                     /gene="ylaC"
                     /locus_tag="ACHK8E_001068"
     CDS             complement(77119..77589)
                     /gene="ylaC"
                     /locus_tag="ACHK8E_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP0343368.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(77705..78256)
                     /gene="maa"
                     /locus_tag="ACHK8E_001069"
     CDS             complement(77705..78256)
                     /gene="maa"
                     /locus_tag="ACHK8E_001069"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP0343369.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(78429..78647)
                     /gene="hha"
                     /locus_tag="ACHK8E_001070"
     CDS             complement(78429..78647)
                     /gene="hha"
                     /locus_tag="ACHK8E_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP0343370.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(78673..79047)
                     /gene="tomB"
                     /locus_tag="ACHK8E_001071"
     CDS             complement(78673..79047)
                     /gene="tomB"
                     /locus_tag="ACHK8E_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP0343371.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        19219 a        19679 c        21259 g        19161 t
ORIGIN      
        1 tgcgcaaagt atttcctttg tcataaaaat aatacttcca gacactatga agttgtgaaa
       61 cataatgtta acttctccat actttggata aggaaataca gacatgaaaa atctcattgc
      121 tgagttgtta tttaagcttg cccaaaaaga agaagagtcg aaagaactgt gcgcgcaggt
      181 agaagctttg gagattatcg tcactgcaat gcttcgcaat atggcgcaaa atgaccaaca
      241 gaggttgatt gatcaggtag agggggcgct gtacgaggta aagcccgatg ccagcattcc
      301 tgacgacgat acggagctgc tgcgcaatta tgtaaagaag ttattgaagc atcctcgtca
      361 gtaaaaagtt aatcttttca acagctgtca taaagctgtc acggccgaga cttatagtcg
      421 ctttgttttt atttttaatg tatttgtaca tggagaaaat aaagtgaaac aaagcactat
      481 tgcactggca ctcttaccgt tactgttttc ccctgtgaca aaagcccgga caccagaaat
      541 gcctgttctg gaaaaccggg ctgctcaggg cgatattact gcacccggcg gtgctcgccg
      601 tttaacgggg gatcagaccg ccgctctgcg tgattctctt agcgataaac ccgccaaaaa
      661 tattattttg ctgattggcg atgggatggg ggactcggaa attactgctg cacgcaatta
      721 tgccgaaggt gcgggcggct tttttaaagg tatcgatgca ttaccgctta ccgggcaata
      781 cactcactat gcgctgaata aaaaaaccgg taaaccggac tacgtcaccg actcggctgc
      841 atcagcaacc gcctggtcaa ctggtgtcaa aacctataac ggtgcgctgg gcgtcgatat
      901 tcacgaaaaa gatcacccaa cgattctgga aatggcaaaa gccgcaggtc tggcgaccgg
      961 taacgtttct accgcagagt tgcaggatgc cacgcccgcc gcgctggtgg cgcatgtgac
     1021 ctcgcgcaaa tgctacggtc cgagcgcgac cagtgaaaaa tgtccgggta acgctctgga
     1081 aaaaggcgga aaaggatcga ttaccgaaca gctgcttaac gctcgtgccg atgttacgct
     1141 tggcggcggc gcaaaaacct ttgctgaaac ggcaaccgtc ggcgaatggc agggaaaaac
     1201 gctgcgtgaa caggcacagg cgcgtggtta tcagttggtg agtgatgctg cctcgctaaa
     1261 tgcggtgacg gaagcgaatc agcaaaaacc cctgcttggc ctgtttgctg acggcaatat
     1321 gccagtgcgc tggctaggac cgaaagcaac gtaccacggc aatatcgata agcccgcagt
     1381 tacctgtacg cctaatccgc aacgtaatga cagtgtaccg accctggcgc agatgaccga
     1441 caaagccatt gaattgttga gtaaaaatga gaaaggtttt ttcctgcaag ttgaaggtgc
     1501 atccatcgat aaacaggatc acgctgcgaa tccttgtggg caaattggcg agacggtcga
     1561 tctcgatgaa gccgtacaac gtgcgctgga attcgctaaa aaggatggca acacgctggt
     1621 catagtcacc gctgatcacg cccacgccag ccagattgtc gcgccggaca ccaaagcgcc
     1681 gggcctcacc caggcgctaa ataccaaaga tggcgcagtg atggtgatga gttacgggaa
     1741 ctccgaagag gattcacaag aacataccgg cagtcagttg cgtattgcag cgtatggccc
     1801 acatgccgcc aatgtcgttg gactgaccga ccagaccgat ctcttctaca ccatgaaagc
     1861 cgccctgggg ctgaaataaa accgcgcccg gcggtgaatt ttcgctgtcg ggtggttttt
     1921 ttgctgttag caaccagact taatggcaga tcacgggcgc aaacgctcat ggttaaaaca
     1981 tgaagaggga tggtgcgatg aaaataacat tactggttac cttgcttttc ggactggttt
     2041 ttttaaccac cgtcggcgct gccgagagaa ccttaacccc gcaacaacag cgtatgacct
     2101 cctgtaatca gcaggcgacg gcgcaggcgt tgaaagggga tgctcgtaag acctacatga
     2161 gtgattgcct gaagaacagc aagtctgcgc ctggtgaaaa aagtttgacg ccacagcagc
     2221 aaaagatgcg cgaatgtaat aatcaagcaa cgcaacaatc tctgaaaggg gatgatcgta
     2281 ataagtttat gagcgcctgc ctcaagaaag ccgcctgata cctgatagtg ctaacgggtg
     2341 agctacgaaa atggctcacc cgaaatttca tacttctgcc tttagccccg tctctataat
     2401 ttgggaaaat tgtttctgaa tgttcccaaa aataatgaat gatgaaaact ttttcaaaaa
     2461 agcggcggcg catggggagg aacctccttt aactcctcaa aacgaacatc agcggtccgg
     2521 gctgcgcttt gcccgtcgcg tcagactacc ccgcgcggtt ggcctggctg gcatgttctt
     2581 accgattgct tcaacgctgg tttcgcatcc gtcgccgggc tggtggtggc tgttgttggt
     2641 cggctgggcg ttcgtctggc cgcatttagc ctggcaaata gcgagcaggg ccgtcgatcc
     2701 gcttagccgg gaaatttaca acttaaaaac cgatgcagta ttagcgggaa tgtgggtagg
     2761 cgtaatgggc gtaaacgcac tgccttccac cgcgatgttg atgattatgt gtctgaattt
     2821 gatgggggca ggcggccccc gtctgtttgt cgcgggtctg gtgttgatgg tggtctcctg
     2881 ccttgtcacc ctcgagctga cgggcatcac cgtgtcgttc aatagcgcac cgctggaatg
     2941 gtggctctcc cttcccatta ttgtcattta tcctctgctg tttggctggg tcagctacca
     3001 gacggcgact aaactggcgg aacataaacg caggttgcag gtcatgagta cccgcgacgg
     3061 tatgacgggc gtgtataacc gacgtcattg ggaaactatg ttacgcaatg aatttgataa
     3121 ctgtcggcgg cataatcgcg atgcaacgtt actgattatc gatatcgacc atttcaagag
     3181 catcaacgat acctggggcc atgatgtggg tgatgaagcg attgtggcgc ttacccgaca
     3241 gttacaaatt accctacgcg gtagcgatgt gattggtcgg tttggcggcg atgagtttgc
     3301 agtaatcatg tccggtacgc cagcagagag cgccatcacc gccatgttac gggtgcatga
     3361 agggctaaac acgttacgct tgccgaatac gccacaggta actttacgga ttagtgtggg
     3421 ggttgcgccg ctgaatccac aaatgagtca ctatcgcgag tggttgaaat cggcagattt
     3481 ggcgctctac aaagcaaaga aagccggacg taaccgcacc gaagtggcgg cctgacgtcc
     3541 ggcaaaagcc atcaggattt gctgagtttt tctgattttt ccatacactt cgtcatcgct
     3601 tcgatcactg cggcacggaa gcctttctct tccagtacgc gtaccgcctc aatggtggtg
     3661 cctcccggtg agcagaccat atctttcagt gttcccggat gttctcccgt ttccagcacc
     3721 atttttgcgg aacccatgac cgcctgagcg gcaaatttat acgcctgggc gcgtggcatc
     3781 ccgcccagca cggcggcgtc ggccatcgct tcgataaaca taaagacgta ggctggcgaa
     3841 gaaccgctca caccgaccac cgggtggatc atcggctcag caatcacttc cgcttcgcca
     3901 aagcagcgga aaatattcag cacatcagcg gtatcttctg gggttaccag cgcgtttggc
     3961 gttacggagg tcatcccggc attaaccagc gcgggagtgt tcggcatggc gcggataatt
     4021 ttccggtcat ggcccagcgc gcgggcaagc tggtcgagcg tgatacctgc agcaatagaa
     4081 acgaccagag agtctttatt caggctggag gtgatttcgc taagcacttt aatcatgatg
     4141 cctggtttaa cggcggcaaa aatgatgtcg gcgatttgcg ctacttcttg cgccgattct
     4201 gcggcgttga tgccgaactg gtcatgcagg gcggtgactt tatccgggga gggggtatat
     4261 acccagattt gccctggaag cacctgaccg ctggcaatca gaccgccgag aatggctttt
     4321 cccatattgc cgcagccaat aaaaccgatt ttcttttcca ttgcctcact cctgccgtga
     4381 aattcattgt tttgataatc gctggcagaa gcataaacag aactatgccg gaaggcaaaa
     4441 gcgcgacaca atagaggatt acccaacaaa ggatgacttt atgacaattt gggtggatgc
     4501 cgacgcgtgt cccaatgtaa ttaaagagat tttgtatcgc gcggcggaac gtatgcagat
     4561 gccgctggta ctggtagcaa accagagttt acgcgtgccg ccatcgcgat ttattcgtac
     4621 gctgcgcgtc gcggcaggtt tcgacgttgc tgataacgaa attgttcggc agtgtgaagc
     4681 gggcgatttg gtgatcaccg cagatatacc tttggctgct gaagccatcg agaaaggcgc
     4741 ggcggcgctt aatccgcgcg gcgaacgtta cacgccagcg accattcgtg agcgcctgac
     4801 gatgcgcgat tttatggata ccttacgtgc cagtgggatc cagaccggag gaccagatag
     4861 tctttcacaa cgtgaccgcc aggcttttgc tgcggagctg gagaagtggt ggctggaagt
     4921 gcaacgtagt cgtggctaaa tgtaatttat tatttacact tcattcttga atatttattg
     4981 gtatagtaag ggatgtattg agattttcac tttaagtgga attttttctt tacaatcgaa
     5041 attgtactag tttgatggta tgatcgctat tctcatgaca ccggctttcg ccgcattgcg
     5101 acctattggg gaaaacccac gatgacacaa cctctttttc tgatcgggcc tcggggctgt
     5161 ggtaaaacaa cggtcggaat ggcccttgcc gattcgctta accgtcggtt tgtcgatacc
     5221 gaccagtggt tgcaatcaca gctcaatatg acggtcgcgg agatcgtcga aagggaagag
     5281 tgggcgggat tccgcgccag agaaacggcg gcgctggaag cggtaactgc gccatccacc
     5341 gttatcgcta caggcggcgg cattattctg acggaattta atcgtcactt catgcaaaat
     5401 aacgggatcg tggtttattt gtgtgcgcca gtatcagtcc tggttaaccg actgcaagct
     5461 gcaccggaag aagatttacg gccaacctta acgggaaaac cactgagcga agaagttcag
     5521 gaagtgctgg aagaacgcga tgcgctatat cgcgaagttg cgcatattat catcgacgca
     5581 acaaacgaac ccagccaggt gatttctgaa attcgcagcg ctctggcaca gacgatcaat
     5641 tgttgatttt cgagcgccta tacttaacgt tcatcccgtg aaataaggaa gaacgatgcc
     5701 aacgaaaccg ccttatcctc gtgaagcata tatagtgacg attgaaaaag gaaagccagg
     5761 acagacggta acctggtacc aactcagagc cgatcatcct aaaccagact cgttgatcag
     5821 tgaacatccg accgctcagg aagcgatgga tgcgaaaaaa cgctatgagg accctgacaa
     5881 agagtgaccg catcagactg ctcggaaggg attctgagtg ccgccacaag ggatctgcgt
     5941 cacatttttc acaattcatg tttttcaaat aattagaata ttaaacaata acaatccatt
     6001 actggaatca tttggaatct ttacattatg cagtgcaggt ctgctgctac gctttttgtc
     6061 atttgtagca caagtaagtg tcagcagtgg tgcttaacac ttgcccggta attaacgacg
     6121 aaataaaagt aaggtggaag aacaatgagt gcgtcgttgg cgatcctgac catcggcatt
     6181 gtacctatgc aggaagtttt gccgctcctg acagaataca ttgacgaaga taatatttcc
     6241 catcatagcc tgctggggaa gttaagccgc gaagaagtga tggcggagta cgcgccagaa
     6301 gcaggcgaag acaccattct cacattatta aatgacaacc atctggccca tgtttcgcgt
     6361 cgcaaagtgg agcgagacct gcaaggtgtg gttgaagtgc tcgataatca gggttacgac
     6421 gtcattatat taatgagtac agcaaacatt agtagtatga ccgcgcgtaa tacgatcttt
     6481 cttgagccgt cgcggatatt gccaccgctg gtttcctcta ttgttgaaga tcatcaggtg
     6541 ggggttatcg taccggttga ggagttgctg accgttcagg cgcaaaaatg gcaaattttg
     6601 cagaaaccgc cagtattttc attgggtaat cccattcatg attcggaaca aaaaatcatt
     6661 gatgccggga aagaattact ggcaaaaggt gcagatgtca tcatgctgga ttgtttggga
     6721 tttaaccagc gtcatcgcga tttactgcaa aaacagctcg atgttcctgt cttgctctct
     6781 aacgtattga ttgcacggct ggctgcggaa ttactgatgt aattttgcgt gacagccagc
     6841 gcctctggcc cctatagtga agtagatgtt caactaccag acagggccag tttatgcttc
     6901 aaagtaatga gtacttttcc ggcaaagtga aatcgatcgg cttttccagc agcagcactg
     6961 gtcgcgccag cgtgggtgtt atggttgaag gtgaatacac cttcagcacc gctgagcctg
     7021 aagagatgac ggtaatcagt ggcgcgctga atgtgttact gcctgacgcg accgactggc
     7081 aagtgtatga agccggttcg ctgtttaatg ttcccggtca cagcgagttt catctgcaag
     7141 ttgccgaacc cacctcttat ctgtgccgct atctgtaatt cctcgccttc cccttgaacg
     7201 ggagggcatt tttctgaaat gtcctctctt taacccataa taatatttcc tttgctggga
     7261 ttttttcgat ttccgatata ttcataattc atcaaggttg atataaatat cagcgaagat
     7321 ctccagatac tattgcggaa ctggcgacgt taaaagataa atcagatgat gaatggtggt
     7381 gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattgcac aacgcacata
     7441 ctccggtgtt gttaacgaca ttgactgagc ctcaggagcg gtcgctggct attaataatt
     7501 cacagctggc tgccgatgtg aaaacggcgt ggttaaaaga ggagccatca ttactcttat
     7561 ttgtcgaaca acccgatctt tcgctgttac gtgatttagt gaaaaacggg gcaacgcgaa
     7621 aaattcgcag tgaagcgcgt caccggcttg aggaaaaaca atgacacaac gcccctggag
     7681 caagttacag cataaaacgc ataatattgc tgcgctaaga attattgccc gctgtagcta
     7741 acgattatat tcctggtgtg gcttcgtacg ccggataaga cgcggcaggc gtcgcatccg
     7801 gcattaaagg taaatcagca attaacgttg tgcttcgcca cctaatcctt caatcaggtt
     7861 ttgaattaac gctgccagtt caccagtcat caggatgaaa tcggcatcaa agcgctgggc
     7921 gaaatcttca cggtcgatat cttcgttttg atcgcgcagc tcgtcgcaga acttcagacg
     7981 cttgagcgaa ccatcgtcgc acatcacaaa ctgaatgcgc tgctgccagt cgagcgccag
     8041 tttagtcacc acttttccgg cttcaatgtg gttggtgatc tcttcgctgg tcagatcttg
     8101 tttcttcgcg cggatcactc cgccatcttc cagcaacgat ttcagctcgg cttcatcaag
     8161 cagctggaag ccctgtgccg cgctgccgga gcgaacccat tcggtcagcg tcagttcaat
     8221 cgggttttcc atgctcaatg gtacaaccgg taacgacccc aggcttttac gcagcagtgc
     8281 cagcgtatct tccgcttttt tggcactggc gcagtccacc ataatcaaac cgttaaccgt
     8341 gtcgatccac atcattgtct ggctaaaacg gctgaaagca cgcggtagca gagagtgcag
     8401 cacttcgtct ttcagcgaat ctttttcggt tttcttcagc ttacgcgcct gttccgcttc
     8461 cagtttggcg attttcgctt ccagcgcctg tttaatcacc ggagacggga ggattttttc
     8521 ttctttgcgc gcgcagataa caatttgacc attggcaacg tgcgttaacg catcgctgtg
     8581 cgatcccatc ggaggaaccc agcccatctt cgccatgtcc tggctgccgc atggggtaaa
     8641 tgccatcgag gctagctgtt tttccatctc ttctgcacgc aacgaaatct cgcggctaag
     8701 acggtaaacc attaaatttt tgaaccacag catgataatt tccacggcct tgtcgttaaa
     8761 tttagcgggc atgataacga attgtcggcg gccttgcatt gccaatccag ttgtccgtct
     8821 ctacgctatt gatattgaaa aaaataagga gagtaccgtg cgtataggta tcgatttagg
     8881 cggcaccaaa actgaagtga ttgcactggg cgatgcaggg gagcagttgt accgccatcg
     8941 tctgcccacg ccgcgtgatg attatcggca gactattgaa acgatcgcca cgttggttga
     9001 tatggcggag caggcaacag ggcagcgcgg aacggtaggt atgggcattc ctggttcgat
     9061 ttcgccctac accggcgtgg tgaagaatgc caattcaacc tggctcaacg gtcagccatt
     9121 cgataaagac ttaagcgcga ggttgcagcg ggaagtgcgg ctggcaaatg acgccaactg
     9181 tctggcggtt tcagaagcag tagatggcgc ggcagcggga gcgcagacgg tatttgccgt
     9241 gattatcggc acgggatgcg gcgcgggcgt ggcattcaat gggcgggcgc atatcggcgg
     9301 caatggcacg gcaggtgagt ggggacacaa tccgctaccg tggatggacg aagacgaact
     9361 gcgttatcgc gaggaagtcc cttgttattg cggtaaacaa ggttgtattg aaacctttat
     9421 ttcgggcacg ggatttgcga cggattatcg tcgtttgagc ggacatgcgc tgaaaggcag
     9481 tgaaattatc cgcctggttg aagaaagcga tccggtagcg gaactggcat tgcgtcgcta
     9541 cgagctgcgg ctggcaaaat cgctggcaca tgtcgtgaat attctcgatc cggatgtgat
     9601 tgtcctgggg ggcgggatga gcaatgtaga ccgtttatat caaacggttc cgcagttgat
     9661 taaacaattt gtcttcggcg gcgaatgtga aacgccggtg cgtaaggcga agcacggtga
     9721 ttccagcggc gtacgcggcg ctgcgtggtt atggccacaa gagtaaaaaa accaggcaat
     9781 tagcgcatca tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa aatgagcagc
     9841 ccgtaggccg gataaggcgt ttacgccgca tccggcaata agcaacgcgc acggtgacag
     9901 taatctaccc cagtggtttc gccagcaccg gactatccgc cgcttgctgg cgcttatagc
     9961 gaccatacag cagcaacgac gacatcgcag caaacgaaag cagggcggca ggcagcgcca
    10021 cgtaattata tgccagcccc agcgtcagca tcataccacc gcaatatgcg ccgacggcgc
    10081 taccgaggtt aaacgctatt tgcccacctg cggcacctaa taactctccg cctttggcat
    10141 tttgcagtaa caatatttgc agtggtgctg aaagggcaaa taatcccgcg caacaaataa
    10201 atgcaaaaat aagcgacgtt gttttcatgc cgccgaagaa aaagagcatc agcagtgcca
    10261 gcacaataat aaagtcagtt actgccgcaa tgcgcagtgg tgaataacgt cctgaaatcc
    10321 ggccacttag cacattcccc agcaccatcc ctagcccaac taacatcata ataaaagtca
    10381 tcgccgtttc cgaaaaaccg gaaataaaca tcatgtatgg ctttacgtag ctgaaccagg
    10441 caaatacacc tgcgttgcca aacatggtgg cggcgaaaat taaccacggg gccgggctgc
    10501 gtaaaaagtg aaattgttcg cgcagctttc ctttcgcttc gtcgcgaata tccggcaccc
    10561 aaaaatagac cgatgccatc accgcaatat taaaaacagc gatcaataaa aaggtgtagc
    10621 gccagctaaa ttcctgactt aaatacgttc ccagcggaat gcccagcaaa ttggcgactg
    10681 tcatcccgga aaccatcccc gccacggcgg cggtgacttt tccgggtttg ataatttttg
    10741 ataacacgat cgcgccgacg ccaaaaaatg cgccatgcgg aaagccggat accagccgac
    10801 caatggcgag catcaggtaa gacgaagaga gcgtgaacat ggcgttgcca atgacgcaca
    10861 acgccaccag aaacaataag atatgtttga gtgagtagcg gctggaaaag agtgcgatga
    10921 ttggcgcacc gaccaccacc cccagcgcat aatacgagat catatgcccg gcagcaggaa
    10981 tcgaaattcc tacgttatgc gccagctccg tgagcacgcc cataatgcca aattcggcca
    11041 tccccaaacc aaacgtgccc agagccaaag ataaaatgac ttttttcata ccaccatcac
    11101 aaccaccaga acggctacca atcaccgtag ccaataaagt gatagcttgc aggataacga
    11161 ccagcaaggc caagagataa ttcgcccctc tgtattcatt atcctgctga atagttattt
    11221 cactgcaaac gcactttcca gtttgctgta gcccaggccg ttgatcttct tcactttgat
    11281 ctgcaccgga atacgctctt tcatcgcttc aacgtggcta atcacaccga tggttttgcc
    11341 actggcattc agggcatcca gcgcatcaag ggcggtatcc agcgtttcgc tatccagcgt
    11401 gccaaaacct tcatcaagga acagcgagtc aatacgcgtt ttatggctga ccagatccga
    11461 aagcgccagc gccagcgcca gactgacgag gaaactttcg ccgccggaaa gggtacgggt
    11521 atcgcgtacc gcatctgcct gccaggtatc aacaacctcg acttccagcg cctcgctggc
    11581 tttgcgctgt aacagatagc gcccgtgcag ccgggtaagt tgctgattag cgagatggac
    11641 taaattatcc agcgtcagcc cctgggcaaa cttgcggaat ttatcgccct ctttggaacc
    11701 tattagcgaa ttcagatatc cccagtcctc aacctgctgc gtcatttgag caatttgctg
    11761 cattaaggtt tgttgttgct gacggttgtc tgcatcctgc ttcagctgct ggcgaatctc
    11821 gccctgactg gtggtgtttt cacgcaactt ttggtgagtt tgcgctaact cttgctgaat
    11881 ctgctccatc gtcacagtga gagccaaccc gtcaggtcgg tgttgctgat gctgtgccag
    11941 cgtttctgct gtctgagtga ccagcgtttg cgcctgacgg cgctggtttt ccagattctg
    12001 cttgagctgt tccagttgcg ttagtgtttg ttcatccatt agcgccgcaa ggaacgcctg
    12061 ctgatcgtca aagacgctgg cctgtagcgc ggtgtcaaac tgcgcctggg ctttttgcag
    12121 actttgcgcc gccagaacgt cctgttgctg taacgtctgc tgctggctgt gtaatgcgag
    12181 acattgttca tgaacctgcc gccagttttc cagcaccaca gtttcttcgc tgtgcgggag
    12241 ttcatcactt tgcggcaacg tttccagaat cggcgtcagc tgctgcatac ggttttgcag
    12301 cgcggttaat tcgttctggc gatgctgcca gctctgcgct tcttgctgac gcgtcgccaa
    12361 ccagctctct tcttcatctt cctgtggcaa tgtcagtgca taacccgcca atgccgttaa
    12421 gagctgctgc tggcgttgtt caatttgctg ttgatactgg ataatttgct gattatgcgc
    12481 ggcaatctgc ccttgtaatt catgtcgttg gctgagtaac cgcagctggc gttcgtgctc
    12541 atcttgtgca tccagccacg gttgaatatc gtcctgtggc tgcaaggtga tattgaggct
    12601 ggccgtgacg gcttgccatt gttgagtaag tgcttgctca tcttgtcgga ggctttgcgc
    12661 ttcgttttca tcgcgctgaa gttgctttgt taaggcgtcc agttgcccac gtagcgtcgc
    12721 accttcttca ccgagctttt taacttcgtt ttccagcgcc agtaatcgag actgattaac
    12781 gccaggctcc agcgcctgat acgcctcgac cgccgggtgg ctggtggaac cacaaagtgg
    12841 acaaggctga cccgcctgta actgtgcacg ttgagcttcc agcgttttga tgcgcgcttc
    12901 ctgctcgcaa atagttttca catcggcaag ttgctgcgtc ttttctttat aacgctggcg
    12961 catttcgtta agtgcggcgt tacgttgcgt ctgttcttgc gtgacattct ggatagcgac
    13021 ctgtaattgc gccaggcgtt tttgttgggg aacaatctgt ccatgcagcg cgaccaggcg
    13081 ctgacgcagc gtgcgttgct cagcatgttg cgccagggcg gtagcaactt catcggcggt
    13141 taacgtcaac gtgatcgccg taagcgcatt aagtttttgc tcagcatggg ttaactgttg
    13201 ctgccattgc cgcagatgct cgcgaacgct ggtttgttgg gagaactgcg cacgccaacc
    13261 cgccagttcg ttgttccact gacggaagcg gtcgtgttcc tgtaaccagg cattcaggct
    13321 ttgttgctgc tgctgtaatt ctgctgactg cttcgccgcg tggtggcgaa tgctcgcccg
    13381 aagcgccatt gtgctctgta agcgagtatt tacttcttta atctgctggc gagtatgcgc
    13441 cagcgccgcg ctgtgttctg cgatgcgttc ccagtgcgga cgaagatttc gtgccggttg
    13501 tgccagactg agcgccgcca gttgaggttg cgctttttct tcttcggcta acgcctgttg
    13561 caaggcctgc tgacggcggc tggcttcttg ctgcaattcg tccagacgcg ttaaccagtt
    13621 tagtgattgt tgttcttgct gctgcgcggt aagtaactgt ttttcttcgt cagtaagtac
    13681 ctgcaaactc gctgtcagcg attgcacttg ttccggcgtg agcaacgcga cgccgctggc
    13741 ctgcgcttgc agcttctcta gctcagtgcg ggccgatttg tgctgctcaa acaccatcga
    13801 cgagatttgt ccgtagattt cagtgccggt taactcctcg agcaattccg cgcgttcttt
    13861 gggtttggca ttcaggaagg cagcaaattg cccctgcgaa agcagcatcg aacgggtgaa
    13921 gcgcccgtaa tccagtccgg ttaacgtcgc cgtcagttcc agcttatctt tcactttgtc
    13981 ggcgagaatt ttgccatcgg cgcagcgcgc cagctctacg cgtggcacct gcaaattacc
    14041 gtcgggctgg ttacgcgccc gattctggct ccagaacgcg cgatacgctt cacctttcac
    14101 ttcaaactcc acctccgcca gacattcggc ggtatcgcgg gtcatgagat cattttgcga
    14161 ttgtgaaacg ttagaaaggc gcggagtttc gtgatacagc gccagacaaa tggcgtccag
    14221 caggggggtt ttccccgcac cggttgggcc ggtaatggca aacagcccgt tgctggcgaa
    14281 cggctcgcgg gtgaaatcaa tcttccattc gccttttaat gagttcaggt ttttcaggcg
    14341 caggctgaga attttcatgc ttcgtgttct ccggcgaggc tatgcaacgt cgtggcgaaa
    14401 agatgctgca gacgttgctg ctgcgattcc tccagttctt ccagtgccag acggcgattg
    14461 aacacctctt cgacgctgag ttcgctgagg gtttcacgct gttggctggc taacacacgc
    14521 tcgcgctgtt cacgactccg gcgtaccagc aatacttcga caggcaatga ttcggttaat
    14581 gcctggattt tgcgctgaat atcatgcaga tactcatcag tagtgatttc gatatccagc
    14641 cagacaggtg gctcctgtga tacatcgcgc cactgttcca gctgttcggt aatcgacgcc
    14701 agatcgcctt tcagcaccgc catgggttgc gttaccggta cgttcaggtt ttccacgctc
    14761 tctaatttgc cgtttgaaaa tgtcacaaga tggacatatt tactcttacc gcattcatca
    14821 aaactcagtg gaatggggga accgcaatat cgaacatgtt ccatgccgcc aataatctgt
    14881 gcgcggtgaa tatgcccgag cgcgatgtag tcggctggtg gaaagttttg tgccggaaac
    14941 gcgtccagcg tgccaatata gatgtcacgc acggcatcac ttttactggc acccacggtc
    15001 gttaaatgtc ccgtggcgat gatgggcaga ggctgatcgc cgcgcagttt gcaggcatcg
    15061 gcatagtgtt gttgataata atcagtaata gccgccagca aatgctgctg tttttcaata
    15121 ccgttaagcc ccgcttggct ggtaataatg tcacgtgggc gtaaaaacgg aatggggcac
    15181 agtactgcgc ctggtgtccc gtcgcgacga ggcaagattt gcggcgcatg tccggcgctg
    15241 gcgaccacgg ttgtattgag gaacgccatg atatcgcgcg attcattcag cgtggcgacc
    15301 gagtcatggt ttcctgccag taccaccaga tgacagccag tttgctgtaa attgacaaca
    15361 aaacggttgt ataacgtgcg ggcgtaactg ggcggcgagc cggtatcgaa aacatcaccg
    15421 gcaacaataa tcgcatccac ctgatgggct tgtgctgtct ccagcagcca gtcaagaaaa
    15481 gcctgatgtt cggcttcgcg gcttttactg tagaagttct ggccgagatg ccagtctgag
    15541 gtgtgaagga tgcgcataac ggttccctgg cgaaaaagca tgggcgcgat tatacccaaa
    15601 cagatgtgcc atttgctttt ttctgcgcca cggaaatcaa taacctgaag atatgtgcga
    15661 cgagcttttc ataaatctgt cataaatctg acgcataatg acgtcgcatt aatgatcgca
    15721 acctatttat tacaacaggg caaatcatgg cgagacgtat tctggtcgta gaagatgaag
    15781 ctccaattcg cgaaatggtc tgcttcgtgc tcgaacaaaa tggctttcag ccggtcgaag
    15841 cggaagatta tgacagtgct gtgaatcaac tgaatgaacc ctggccggat ttaattctcc
    15901 ttgactggat gttacctggc ggctccggta tccagttcat caaacacctc aagcgcgagt
    15961 cgatgacccg ggatattcca gtggtgatgt tgaccgccag aggggaagaa gaagatcgcg
    16021 tgcgcggcct tgaaaccggc gcggatgact atatcaccaa gccgttttcg ccgaaggagc
    16081 tggtggcgcg aatcaaagcg gtaatgcgcc gtatttcgcc aatggcggtg gaagaggtga
    16141 tcgagatgca ggggctaagc ctcgacccga catctcaccg ggtgatggcg ggcgaagagc
    16201 cactggagat ggggccgaca gaatttaaac tgctgcactt ttttatgacg catcctgagc
    16261 gcgtgtacag ccgcgagcag ctgttaaacc acgtctgggg aactaacgtg tatgtggaag
    16321 accgcacggt cgatgtccac attcgtcgcc tgcgtaaagc actggagccc ggcgggcatg
    16381 accgcatggt gcagaccgtg cgcggtacag gatatcgttt ttcaacccgc ttttaacgcc
    16441 ttgctcatcg gacgcagagc agggcttatg atttcttaac tggagtatct tacgtgctgg
    16501 aacggctgtc gtggaaaagg ctggtgctgg agctgctact ttgctgcctc ccggctttca
    16561 tcctgggtgc attttttggt tacctgccct ggtttttgct ggcatcggta acaggactgc
    16621 ttatctggca tttctggaat ttattgcgcc tttcatggtg gctgtgggtg gatcgcagta
    16681 tgaccccgcc accggggcgt ggtagctggg aaccgctact atacggctta caccagatgc
    16741 agctgcgaaa taaaaaacgc cgccgcgaac tgggcaatct gattaaacgc tttcgtagtg
    16801 gcgcggagtc gctgcccgac gcagtagtgc tgaccacgga agagggcggt attttctggt
    16861 gtaacggtct ggcgcaacag attcttggtt tgcgctggcc ggaagataac gggcagaaca
    16921 tccttaacct gctgcgttac ccggagttta cgcaatatct gaaaacgcgt gatttttctc
    16981 gcccgcttaa tctggtgctc aacaccgggc ggcatctgga aattcgcgtt atgccttata
    17041 cccacaaaca gttgctgatg gtggcgcgtg atgtcacgca aatgcatcaa ctggaagggg
    17101 cgcggcgtaa cttttttgcc aacgtgagcc atgagttacg tacgccattg accgtgttac
    17161 agggttacct ggagatgatg gatgagcagc cgctggaagg cgcggtacgc gaaaaagcgt
    17221 tgcacaccat gcgcgagcag acccagcgga tggaagggtt ggtgaagcaa ttgctgacgc
    17281 tgtcgaaaat agaagccgca ccgacgcatt tgctcaatga aaaggttgat gtgccgatga
    17341 tgctgcgcgt cgttgagcgc gaggctcaga ctctgagtca gaaaaaacag acatttacct
    17401 ttgagataga taacggcctc aaggtgtcag gcaacgaaga tcagctacgc agtgcgattt
    17461 cgaacctggt ctataacgcc gtgaatcata cgccggaagg cacgcatatc accgtacgct
    17521 ggcagcgagt gccgcacggt gccgaattta gtgttgaaga taacggaccg ggcattgcac
    17581 cggagcatat tccgcgcctg accgagcgtt tttatcgcgt tgataaagcg cgttcccggc
    17641 aaaccggtgg tagcggatta gggttagcga tcgtgaaaca tgcggtgaat catcacgaaa
    17701 gtcgcctgaa tattgagagt acagtaggaa aagttacacg tttcagtttt gttatcccgg
    17761 aacgtttaat tgccaaaaac agcgattaat tcgcctttgt catcttttat tgccataagc
    17821 cagccgatgc tggcttattt tctttgcagt cgaaatacgg gcgttagatt ttacaacggt
    17881 tggtgatttt ttgttcgcat gattagccat gtctttttca ttgaaatagt gttttatact
    17941 ggttggtgat ttcttatcgc tatatacctc tggtttttag atcccttctt gctttaaaac
    18001 gttataagcg tttaaattgc gcttcaggtg ctgtcatact gactgcatta acgcggtaaa
    18061 tcgaaaaact attcttcgcc gcgcctggtt gggagtattt cccgctaaaa ttgtttaaat
    18121 ataccgctgt atcatcccca gggattggca caaaaattta acgttacaac accacatcca
    18181 caggcagtat gatttatgac ccatcaatta agatcgcgcg atatcatcgc tctgggcttt
    18241 atgacatttg cgttgttcgt cggcgcaggt aacattattt tccctccaat ggtcggcttg
    18301 caggcaggcg aacacgtctg gactgcggca ttcggcttcc tcattactgc cgttggcctg
    18361 ccggtattaa cggtggtggc gctggcaaaa gttggcggcg gtgttgacag cctcagcacg
    18421 ccaatcggta aagttgctgg cgtactgctg gcaacggtgt gttacctggc ggtggggccg
    18481 ctgttcgcta cgccgcgtac agctaccgtt tcttttgaag tggggattgc gccgctgacg
    18541 ggtgattccg cactgccgct gtttatttac agcctggtct atttcgctat cgttattctg
    18601 gtttcgctct atccgggcaa gctgctggat accgtgggca acttccttgc gccgctgaaa
    18661 attatcgcgc tggtcatcct gtctgttgcc gcaattatct ggccggcggg ttctatcagc
    18721 acggctactg aggcttatca aaacgctgcg ttttctaacg gcttcgtcaa cggctatctg
    18781 accatggata cgctgggcgc aatggtgttt ggtatcgtta ttgttaacgc ggcgcgttct
    18841 cgtggcgtta ccgaagcgcg tctgctgacc cgttataccg tctgggctgg cctgatggcg
    18901 ggtgttggtc tgactctgct gtacctggcg ctgttccgtc tgggttcaga cagcgcgtcg
    18961 ctggtcgatc agtctgcaaa cggtgcggcg atcctgcatg cttacgttca gcataccttt
    19021 ggcggcggcg gtagcttcct gctggcggcg ttaatcttca tcgcctgcct ggtcacggcg
    19081 gttggcctga cctgtgcttg tgcagaattc ttcgcccagt acgtaccgct ctcttatcgt
    19141 acgctggtgt ttatcctcgg cggcttctcg atggtggtgt ctaacctcgg cttgagtcag
    19201 ctgattcaga tctctgtacc ggtgctgacc gccatttatc cgccgtgtat cgcactggtt
    19261 gtattaagtt ttacacgctc atggtggcat aattcgtccc gcgtgattgc tccgccgatg
    19321 tttatcagcc tgctttttgg tattctcgac gggatcaaag catctgcatt cagcgatatc
    19381 ttaccgtcct gggcgcagcg tttaccgctg gccgaacaag gtctggcgtg gttaatgcca
    19441 acagtggtga tggtggttct ggccattatc tgggatcgcg cggcaggtcg tcaggtgacc
    19501 tccagcgctc actaaatcac tgaacatttg ttttaaccac ggggctgcga tgccccgtgg
    19561 ttttttattg tgttgatggg ttaggaattg atggaaagta agaacaagct aaagcgtggg
    19621 ctaagtaccc gccacatacg ctttatggca ctgggttcag caattggcac cgggttgttt
    19681 tacggttcgg cagacgccat caaaatggcc ggtccgagcg tgttgttggc ctatattatc
    19741 ggtggtgtcg cggcgtatat cattatgcgc gcgctggggg aaatgtcggt acataacccg
    19801 gccgccagct ctttctcgcg ttatgcgcag gaaaacctcg gcccgctggc tggttacatt
    19861 accggctgga cctactgctt tgaaatcctt attgtcgcca tcgccgatgt gaccgctttt
    19921 ggtatctata tgggtgtctg gttcccgacg gtgccgcact ggatttgggt actgagcgtg
    19981 gtgctgatca tttgcgccgt aaacctgatg agcgtgaagg tattcggtga gctggagttc
    20041 tggttctcgt tctttaaagt cgccaccatc atcatcatga ttgtcgccgg tttcggcatc
    20101 atcatctggg ggattggcaa cggcgggcaa ccgaccggta ttcataacct gtggagcaac
    20161 ggcggcttct tcagtaacgg ctggcttggg atggtgatgt cgttgcaaat ggtgatgttt
    20221 gcttacggtg ggatcgaaat tatcgggatt accgccggtg aagcgaaaga tcctgagaaa
    20281 tcgattccgc gtgcgattaa ctccgtgccg atgcgtattc tggtgttcta cgtcggtacg
    20341 ctgttcgtca ttatgtctat ctacccgtgg aatcaggttg gcactgccgg tagcccgttc
    20401 gtactgacgt tccagcatat gggcattacc tttgccgcca gcattcttaa ctttgttgtg
    20461 ctgaccgctt cgctgtcggc aattaacagt gacgtatttg gcgtaggccg tatgctccac
    20521 ggtatggcag agcagggcag cgcaccaaaa attttcagca aaacctcgcg tcgcggtatt
    20581 ccgtgggtta cggtgctggt gatgactacc gcgctgctgt ttgctgtgta tctgaactac
    20641 atcatgccgg aaaacgtctt cctggtgatc gcttcgctgg caaccttcgc cacggtgtgg
    20701 gtgtggatta tgatcctgct gtcgcaaatc gccttccgtc gccgtttacc gccagaagaa
    20761 gttaagtcgc tgaaatttaa agtaccgggt ggggtagcaa ccaccatcgg cggtttgatt
    20821 ttcctgctct ttattatcgg gttgattggt tatcacccgg atactcgtat ctcgctgtat
    20881 gtcggtttcg cgtggattgt tgtgctgttg attggctgga tgtttaagcg ccgccacgat
    20941 cgtcagctgg ctgaaaacca ctaatccttg cgttctgtaa gccggttcag gaggaaatcc
    21001 tgatccggct attccgaaag ttatccgccc ccgtcctcct cccccaaata tccttcagat
    21061 gatgagtgat cctgcattag gctatggcaa ggtgatcaga ttttcatcac aggggaatta
    21121 tgatgttaaa tgcatggcac ctgccggtgc ccccatttgt taaacaaagc aaagatcaac
    21181 tgctcattac actgtggctg acgggcgaag acccaccgca gcgcattatg ctgcgtacag
    21241 aacacgacaa cgaagaaacg tcagtaccga tgcataagca gcgcagtcag ccgcagcctg
    21301 gtgttaccgc atggcgggcg gcgatcgatc tctccggcgg actacctcgg cggcgttata
    21361 gtttcaaatt actgtggcac gatcgccagc gttggtttac accgcagggc ttcagccgaa
    21421 tgccgccggc acgactggag cagtttgccg tcgatgtacc ggatatcggc ccacaatggg
    21481 ctgcggatca gattttttat cagatcttcc ctgatcgttt cgcccgtagt cttcctcgtg
    21541 aagctgaaca ggatcatgtc tattaccatc acgcagccgg acaagagatc atcttgcgtg
    21601 actgggatga accggtcacg gcgcaggcgg gcggatcaac gttctatggc ggcgatctag
    21661 acgggataag cgaaaaactg ccgtatctga aaaaacttgg cgtgacggcg ctgtatctca
    21721 atccggtgtt taaagctccc agcgtacata aatacgatac cgaggattat cgccatgtcg
    21781 atccgcagtt tggcggtgat ggggcgttgt tgcgtttgcg acacaatacg cagcagctgg
    21841 gaatgcggct ggtgctggac ggcgtgttta accacagtgg cgattcccat gcctggtttg
    21901 acagacacaa tcgcgggacg ggtggtgctt gtcacaaccc cgaatcgccc tggcgcgact
    21961 ggtactcgtt tagtgacgat ggcacggcgc tcgactggct gggctatgcc agcttgccga
    22021 aactggatta tcagtcggaa agtctggtga atgaaattta tcgtggggaa gacagtatcg
    22081 tccgccactg gctgaaagcg ccgtggaata tggacggctg gcggctggat gtggtgcata
    22141 tgctggggga agcgggtggg gcgcgcaata atttgcagca cgtagccggg atcaccgaag
    22201 cggcgaaaga aacccagccg gaagcgtata tcgttggaga gcattttggc gatgcacggc
    22261 aatggttaca ggccgatgtg gaagatgccg ccatgaacta tcgcggcttc acattcccgt
    22321 tgtggggatt tcttgccaat accgacatct cttacgatcc gcagcaaatt gacgcccaaa
    22381 cctgtatggc ctggatggat aattaccgcg ccggactttc tcatcaacag caactgcgta
    22441 tgtttaatca gctcgatagc cacgatactg cgcgatttaa aacgctgctc ggtcgggata
    22501 ttgcgcgcct gccgctggcg gtggtctggt tgttcacctg gcctggtgta ccgtgcattt
    22561 attacggtga tgaagtggga ctggatggta aaaacgatcc gttttgccgt aaaccgttcc
    22621 cctggcaggt ggaaaagcag gatacggcgt tattcgcgct gtaccagcga atgattgcgc
    22681 tgcgtaagaa aagtcaggcg ctgcgtcgtg gcggctgtca ggtactgtat gcggaagata
    22741 acgtggtggt atttgtccgc gtgctgtatc agcagcgtgt actggtggca atcaaccgtg
    22801 gcgaagcctg tgaagtggtg ctacccgcgt cgccgttgct taatgtcgcg caatggcaac
    22861 gcaaagaagg tcacgggcaa ctgacagacg ggattctggc tttgcctgcc atttcggcta
    22921 cggtgtggat gaattaacgt cttataacgc cttgcgtgac gcctgctcca tcatccgcgg
    22981 ataaaactgc cagaagcagg tttcgagagc atcgtaatgg gcgtctaaat cgtaccagga
    23041 gttacgcagg gcatccagac gtgggcgacg gcttgccatg ccgtttaaca cgctctggat
    23101 gaaatccata tcgcgatagc gcaccaacca ctgctctgac cataagtagt tgttcagatt
    23161 gataaagcgt ggcggtgagt ctggaagaat agtcatcact tgctcccggg cataacaggt
    23221 aaattcctgt agcggaaaat cgggcgacag ctgcgaccag tggcgggaca gaaagtgatc
    23281 ccacatcaca tccagcgtaa taggcgcgac acggcgcgtt tcattacgaa accactcccg
    23341 cgcttcgcgg acttctggca gattgtcggt taatacgtcg attcgccgat gcatatgaat
    23401 gccagccacg acgtcgggcg gaaaactttc ttcaggattt ccgcgtacga aatcagccag
    23461 taaattgccg gaaagcgagc tttccgcgag atgggctaaa tgcaggtgag ctaaaaaatt
    23521 catcgattat attctatcca aaggggggta aaggttgcag ggagagcgcc ccggcactag
    23581 actacccgcc tcttatttta gtctgagtca gtgtcatgcg cgttaccgat ttctcttttg
    23641 aattgcccga atccctgatt gcccactatc ccatgcctga acgcagtagc tgtcgtttac
    23701 tgtcgctgga cgggccgacg ggcgcgctga cgcacggtac tttcaccgat ttactcgata
    23761 agctcaaccc cggcgatctt ctggttttta ataatacccg cgtgatcccg gcgcgtctgt
    23821 ttgggcgtaa agccagcggc ggcaagattg aagtgctggt tgagcggatg ctcgacgaca
    23881 aacgcattct tgcgcatatt cgcgcctcga aagcgccaaa acctggcgca gaactgctgc
    23941 tgggcgatga cgaacgtatt aacgcaacaa tgaccgcgcg ccacggcgca ctgtttgaag
    24001 tcgaatttaa tgatcaacgt tcggtgctgg atattctcaa cagcatcggc catatgccgc
    24061 tgccgccgta catcgaccgt ccggacgaag atgcagaccg cgaactttat caaacggttt
    24121 atagcgaaaa acccggcgcg gtagcagcac cgaccgccgg actgcacttt gacgaacctt
    24181 tgctggaaaa attacgcgcc aaaggcgtgg agatggcgtt tgtgacgttg cacgttggtg
    24241 cgggcacctt ccagccggtg cgcgtcgaca ccattgaaga tcacatcatg cactcggaat
    24301 acgctgaagt gccgcaggat gtggtagacg cggtactggc ggcgaaagcg cgcggtaacc
    24361 gggtgattgc ggttggtacc acctcagtac gttcgctgga aagtgcggct caggcagcga
    24421 aaaacgatct cattgaaccg ttctttgacg atacccaaat ctttatctat ccgggtttcc
    24481 agtacaaagt ggtcgatgcg ctggtgacga acttccacct tccggaatcg acgctgatta
    24541 tgctggtttc ggcatttgct ggttatcaac acaccatgaa tgcctataaa gcggcggtag
    24601 aagagaaata tcgctttttt agttacggtg atgcgatgtt tatcacgtac aatccgcagg
    24661 caattaatga gcgcgttggg gagtaattcc gcggcgctgg tttaaacgtt ggactgtttt
    24721 tctgacgtag tggagaaaaa atgaaatttg aactggacac caccgacggt cgcgcacgcc
    24781 gtggccgcct ggtctttgat cgtggcgtag tggaaacgcc ttgttttatg cctgttggca
    24841 cttacggcac cgtaaaaggg atgacgccgg aagaagttga agccactggc gcacaaatta
    24901 ttctcggtaa caccttccac ctgtggctgc gcccgggcca ggaaatcatg aaactgcatg
    24961 gcgatttaca cgattttatg cagtggaaag ggccgattct taccgactcc ggcggcttcc
    25021 aggtcttcag ccttggtgat attcgtaaaa tcaccgaaca gggcgtgcac ttccgtaacc
    25081 cgatcaacgg cgacccgatt ttcctcgacc cggaaaagtc gatggagatt cagtacgatc
    25141 ttggttcgga tatcgtcatg atctttgatg agtgtacgcc gtatcctgct gactgggatt
    25201 acgcaaaacg ctccatggag atgtctctgc gttgggcgaa gcgtagccgt gagcgttttg
    25261 acagtctcgg aaacaaaaat gcactgtttg gtatcattca gggcagcgtt tacgaagatt
    25321 tacgtgatat ttctgttaaa ggtctggtag atatcggttt tgatggctac gctgtcggcg
    25381 gtctggctgt gggtgagccg aaagcagata tgcaccgcat tctggagcat gtatgcccgc
    25441 aaattccggc agacaaaccg cgttacctga tgggcgttgg taaaccagaa gacctggttg
    25501 aaggcgtgcg tcgcggtatc gatatgtttg actgcgtaat gccaacccgc aacgcccgaa
    25561 atggtcattt gttcgtgacc gatggcgtgg tgaaaatccg caatgcgaag tataagagcg
    25621 atactggccc actcgatcct gagtgtgatt gctacacctg tcgcaattat tcacgcgctt
    25681 acttgcatca tctcgaccgt tgcaacgaaa tattaggcgc gcgactcaat accattcata
    25741 accttcgtta ctaccagcgt ttgatggcgg gtttacgcaa ggctattgaa gagggtaaat
    25801 tagagagctt cgtaactgat ttttaccagc gtcaggggcg agaagtacca cctttgaacg
    25861 ttgattaata ttaataatga gggaaattta atgagctttt ttatttctga tgcggtagcg
    25921 gcaacgggtg caccggcgca aggtagcccg atgtctttga ttttgatgct ggtggtattc
    25981 ggtctgattt tctatttcat gatcctgcgt ccacagcaga agcgcaccaa agaacacaaa
    26041 aagctgatgg actccatcgc taaaggtgat gaagtgctga cgaacggtgg cctggtcggt
    26101 cgcgtaacca aagtagcgga aaacggctac attgctatcg cgctgaatga caccactgaa
    26161 gtagttatta aacgtgactt cgtagctgcc gttctgccga aaggcaccat gaaggcgctg
    26221 taattaaaat ttttccctaa gggaattgcc gtgttaaacc gttatccttt gtggaagtac
    26281 gtcatgctga tcgtggtgat tgtcatcggt ctgctgtatg cgcttcccaa cctgtttggt
    26341 gaggatccgg ctgttcagat cactggtgcg cgcggagtcg ccgccagtga gcaaacgctg
    26401 atccaggtcc agaaaacgtt acaagaagaa aaaataactg ctaagtctgt ggcactggaa
    26461 gagggcgcta ttcttgcgcg cttcgactcc actgacaccc agctgcgcgc tcgtgaagca
    26521 ttaatgggcg ttatgggtga caaatacgtc gtggcgctta accttgcccc ggcaacgccg
    26581 cgctggctgg cagctattca cgctgagccg atgaagctcg gccttgacct gcgtggcggc
    26641 gttcacttcc tgatggaagt ggatatggac accgcgcttg gcaaactcca ggaacaaaac
    26701 atcgatagcc tgcgcagcga cctgcgcgaa aagggcatcc cgtataccac cgttcgtaaa
    26761 gaaaacaact acggcctgag catcactttc cgcgatgcta aagctcgtga tgaagccatt
    26821 gcgtatctga gcaagcgcca tccggacctg gtgattagca gccagggcag caaccagctg
    26881 cgtgcggtaa tgagcgatgc tcgcctgagt gaagcgcgtg aatatgcggt gcagcagaac
    26941 attaatatcc tgcgtaaccg tgtaaaccaa cttggcgtgg cggagccggt ggttcagcgt
    27001 cagggtgctg accgtatcgt tgttgaactg ccaggtattc aggacacagc gcgtgcgaaa
    27061 gagattctgg gtgcgacggc aacgctggaa ttccgtctgg taaacaccaa cgttgatcag
    27121 gccgctgcgg catccggtcg cgtaccgggc gactctgaag tgaaacagac ccgcgaaggt
    27181 cagccggttg tgctgtacaa acgcgtaatt ctgaccggtg accatatcac cgactccact
    27241 tccagccagg acgaatacaa ccagccgcag gttaacatct cgctcgatag cgcgggtggt
    27301 aacatcatgt ctaacttcac caaggacaac atcggcaaac cgatggcaac cctgtttgtg
    27361 gagtacaaag acagcggtaa gaaagatgca aacggtcgtg cggttctggt gaaacaggaa
    27421 gaggtgatta atatcgccaa catccagtct cgtctgggta acagcttccg tattactggc
    27481 atcaataacc caagcgaagc gcgtcagctt tcactgctgc tgcgtgccgg tgcgttgatc
    27541 gcgccgattc agattgttga agaacgtacc attggcccaa ccctgggtat gcagaacatt
    27601 gagcaggggc tggaggcttg ccttgccggt ctgctggttt ctattctgtt catgatcatc
    27661 ttctataaga agtttggtct gattgcgacc agtgctctga ttgccaactt gatcttgatt
    27721 gtcggtatta tgtcgctgtt gccaggcgca acgctgagta tgccaggtat cgcgggtatc
    27781 gtcttaaccc ttgcggtagc ggtcgatgcg aacgtactga tcaacgaacg tattaaagaa
    27841 gagttgagca acggacgtac tgttcaacag gcaattgatg aaggttatcg tggcgcattc
    27901 agttctatct tcgatgcgaa catcaccacg ctgattaaag tcatcatcct gtacgcagtg
    27961 ggtaccgggg caattaaagg gttcgcgatt actaccggta tcggtgtggc gacgtcgatg
    28021 tttaccgcga ttgtcggtac gcgtgccatc gtaaacctgc tatatggcgg caagcgcgtc
    28081 aagaagctgt caatctgagg agtgcgatgt ggcacaggaa tatactgttg aacaactaaa
    28141 ccacggccgt aaagtctatg actttatgcg ctgggactac tgggcttttg gcatctctgg
    28201 tctgctgtta atcgccgcta tcgttattat gggcgtgcgc ggctttaact gggggctgga
    28261 tttcaccggt ggtacggtta ttgaaattac gctcgaaaaa ccggctgaaa ttgacgtaat
    28321 gcgtgatgca ttgcaaaaag ccggttttga agagccgatg ctgcaaaact ttggtagcag
    28381 ccacgacatc atggtccgta tgccgcctgc tgaaggcgaa accggcggtc aggtgctggg
    28441 cagccaggtc ctgaaggtga ttaacgaatc caccaatcag aatgcggcag tgaagcgtat
    28501 tgagttcgtc ggtccgagcg tgggggcaga ccttgcgcaa accggtgcga tggcgttgat
    28561 ggcggcgctg ctgtctatcc tcgtgtacgt aggtttccgc tttgagtggc gactggcggc
    28621 aggggttgtt attgcgctgg cacacgacgt tatcattacg ctgggtattt tgtcgttatt
    28681 ccatatcgag attgacctga ccattgtggc atcgttgatg tcagttatcg gttactcgct
    28741 taacgacagt atcgtggtat cggaccgtat tcgtgaaaac ttccgcaaga tccgtcgcgg
    28801 tacgccttac gaaatcttta acgtgtcctt gacccagacg ctgcaccgta ccttgatcac
    28861 atccggtact accttgatgg ttatcctgat gctgtacctc ttcggtggtc cggtactgga
    28921 aggcttctcg ctgaccatgc ttatcggtgt ttccatcggt actgcatctt ccatctatgt
    28981 ggcatcggcg ctggctctga aactgggtat gaagcgcgaa cacatgttgc agcagaaagt
    29041 ggaaaaagaa ggggcggatc agccgtcaat tctgccttaa tcaagtttca gctgatgttg
    29101 aaaatcccgg tcagaagatc gggatttttt ttgatgtaag gtgattgcga aaatccccgc
    29161 atcttgggaa actgcgcgta accctacatt tcatccaggt aactttttca tggctatcat
    29221 cccaaaaaac tatgcgcggt tagaaagcgg ctatcgcgaa aaagcattaa aaatctatcc
    29281 gtgggtctgc ggtcgctgtt cccgcgagtt tgtttattcc aacctgcgtg aacttaccgt
    29341 tcaccacatt gatcacgacc atactaataa cccggaagat ggcagtaact gggaattact
    29401 ttgtctctat tgccacgatc atgagcattc gaaatacacc gaagcggatc agtatggtac
    29461 gaccgttatc gcaggggaag atgcgcagaa agatgtcggt gaagcgaagt acaacccatt
    29521 cgctgatttg aaagcgatga tgaacaagaa gaagtgataa aaaatgccgg atatgaaatc
    29581 cggcattctc acaattagaa gttgtaacct actaccagat aaccacccca gccggtagag
    29641 cgaacgttga agttgccgtt gccgaagttc agttctgcat cgtcattcca ctgaccaccg
    29701 ttatgccagt aacgggcaac gacagagtag tgccagtgat cgtaattcag cgccagaata
    29761 tggctggacg caatagagtt attggtacgt gttttaatac cgttgttcgc ataaccgctg
    29821 tcatcgccca ggtcggaacc ccagtcgaag ttggtgaagc cgatgtagct cagctggccg
    29881 ccccacagat cggtaatcgg cacaaagtac ttcactttga aacggtaacc gtcccactcg
    29941 ttttcgttcg ctgcgccgta gttctgccac tggtatttcg catagacgtt catggacagg
    30001 ctcatcggca gaccagtatc gatgtcggta cccagaccca tgtaccaggt gctctggcgg
    30061 ccatctttat tacgacccat gtcgtaaatg tagttgttcg cgaagtacca ctctttgaac
    30121 ggaccaaagc taaggtcagt attggtcagc ttgtcgatgg agaaacgcgg ttcgatttcc
    30181 ataaacagcg gagaaccgtg gttccagata ccttttgcat cggagttacc gccgaagaat
    30241 accggcgcat ccgcataacc atagaagtcg aaccagtctt ttttagcgaa tgcttcgtac
    30301 tcaaggtagg tatcgttgcg gagctgcggt ccgaaacggg tgtgatagct tccgacaacg
    30361 ttaacgctct ggtgccacca gtcggaaaga tactgcggtt tgtcgttttc agctgcgttg
    30421 acagtaaaag acgaagagag cgccagtacc gcaccggctg ccagtaatgt ttttttcata
    30481 tgtatgccac tgtttgaaaa tcccttgcgg gagtgaaaaa ggcgcaaatt gcgtttctaa
    30541 atatttcgta tacagaggag cctattataa aaatcattgc tcacaaaaat atgtttcgtt
    30601 tcacagttct atcatttacg taatcgattg cgttcacgtt tacacacatt cgggcgggga
    30661 ttgtactgac tttcaccctt gttgcaaact tttactatca atccaggtga tgtaagcgga
    30721 gtaacaaaat gacggagaaa gatgttgcgg gacgcacaat atcgccccgc aatacgttta
    30781 ctgcataaca ggttgaatgt cgtggatacg tacaaagcct aactgatcca cagacatacc
    30841 ctttaactgt aacggaatat caacatcgct gggggcgagg acgctggcag gtgcgttaat
    30901 caattgattc tgcacattga tttcctgaaa gttgtctatt gttccctgaa tttgcccata
    30961 ttcaacggtt ccgctgaatg cgggtaaagg gtcattggat tcgccctgaa tacgtagcgt
    31021 cagggtagtg ccatctgtgt caggcgtaat attcaccagc gacatacgta aagtaccgat
    31081 ctggctttcc agtcttgctg gtgtttttgc tccaggcaga aggtaaaccc cactgctgga
    31141 ttttgcattc agcttgtttt gctgagtgat tttgaccgtt tctttattga gttgcgtcat
    31201 ctcctggttc agcgtgctga cgctgtgttt catctgtcgc acttcacttt gctgcacaca
    31261 ggcgctaagg ctgaacaggc ttcccagcag gagcagtcga aaaacgttag tattcatagg
    31321 tcgtcccttt ctcgtcgcaa ttagcctaat ggtagagggt aaaagcagtg aaagcatcga
    31381 tcctttatct caaaagcgtt gcgcctttgt tgtatcgtca gttcagggta aaatagattt
    31441 ctgttaacca cctggtcagg acgccgtatg cattgcccat tctgtttcgc cgtggacact
    31501 aaggtaattg actctcgtct cgtgggcgag ggttcatccg tacgccgccg tcggcagtgt
    31561 ctggtgtgta atgaacgttt caccaccttt gaagtggcgg agctggttat gccgcgtgtt
    31621 gtaaaaagca acgacgtgcg tgaaccgttt aatgaagaga aattacgtag cggaatgctg
    31681 cgggcgctgg aaaaacgtcc ggtgagttcc gatgacgtcg aaatggcaat caatcatata
    31741 aaatcgcagc tgcgcgccac cggtgagcgc gaagtgccga gcaagatgat tggcaatctg
    31801 gtgatggagc aattgaaaaa gctcgataaa gtcgcctata tccgttttgc ctctgtctac
    31861 cgcagtttcg aagatatcaa agaatttggc gaagagatcg cgcgcctgga ggactaagcc
    31921 gtgcaggacg agtattacat ggcgcgggcg ctaaagctgg cgcaacgagg acgttttacc
    31981 acgcatccca acccgaatgt cgggtgcgtc attgtcaaag atggcgaaat tgtcggtgaa
    32041 ggttaccatc aacgcgcggg tgaaccgcat gccgaggtac acgcgttgcg catggccggc
    32101 gaaaaagcca aaggcgcaac ggcgtatgtc acactcgaac cctgtagcca tcatggtcgt
    32161 acgccaccgt gttgtgatgc attaattgct gcgggcgtgg cgcgcgtggt tgctgcaatg
    32221 caagacccca atccgcaggt cgctggacgt ggactttacc gtctgcaaca ggctggcatt
    32281 gacgtcagcc acgggctgat gatgagtgaa gctgagcaat tgaataaagg ctttctcaaa
    32341 cggatgcgca ccggctttcc ttatattcag ttaaaacttg gcgcatcgct tgatggtcgc
    32401 acggcgatgg cgagtggcga aagccagtgg atcacttcac ctcaggcgag gcgcgacgta
    32461 caacgactgc gcgcgcaaag tcatgccatt ttaaccagca gcgccacggt gctggcggat
    32521 gatccagcct taacagtgcg ttggtctgaa ctggatgaac aaactcaggg actctacccg
    32581 caacaaaatc tccgccagcc ggtacgtatt gtgattgata gccaaaatcg cgtgacgccg
    32641 gaacatcgca ttgtgcagca gcccggcgaa acctggttcg cgcgtacgca ggaagattct
    32701 cgtgagtgga cggaaacggt gcgtaccttg ctgattccag agcataaagg tcacctggat
    32761 ctggttgtac taatgatgca actgggtaaa cagcagatta acagcatctg ggtggaagcg
    32821 gggccaacgc tcgctggcgc attgctacag gcgggtttag tcgatgagct gattgtctat
    32881 atcgcaccta aactattagg caacgacgcc cgtggattat gcacgctgcc agggcttgag
    32941 aaattagccg acgctcccca atttaaattc aaagagatac gtcatgtagg cccggatgtt
    33001 tgcctgcatt tagtgggtgc atgatctccc ggctcgaaag ggaagcagcg cacgaaatat
    33061 tatgctaaaa tccgcccccc tgcggggcca tactcgaacc cgaaggaaga aaatgaacat
    33121 tattgaagct aacgttgcta ccccggacgc tcgcgtcgcc atcaccatcg cgcgtttcaa
    33181 caactttatc aatgacagcc tgctggaagg tgcaattgac gcactgaaac gtatcggtca
    33241 ggtaaaagat gaaaacatta ccgttgtttg ggtgccaggt gcctatgagc tgccgctggc
    33301 ggcgggtgca ctggctaaaa ccggtaaata cgacgcggtg attgcgctgg gtacggttat
    33361 tcgtggtggc actgcccact ttgaatatgt cgctggtggt gcaagcaacg gcctggcgca
    33421 tgttgcccag gacagcgaaa ttccggttgc ttttggggtt ctgaccactg aaagcattga
    33481 acaagcgatc gaacgtgctg gcaccaaagc tggcaacaaa ggtgcagaag ctgcactgac
    33541 cgcgcttgaa atgattaatg tattgaaagc catcaaggcc tgaaattagt aaggggaaat
    33601 ccgtgaaacc tgctgctcgt cgccgcgctc gtgagtgtgc cgtccaggcg ctctactcct
    33661 ggcagttgtc ccagaacgac atcgctgatg ttgaatacca gttcctggct gaacaggatg
    33721 taaaagacgt tgacgtcctg tacttccgtg agctgctggc cggggtggcg actaacaccg
    33781 catacctcga cggactgatg aagccgtacc tgtcccgtct gctggaagaa ctgggccagg
    33841 tagaaaaagc agtactgcgc attgcgctgt acgagctgtc taaacgtagc gatgtgccat
    33901 acaaagtggc cattaacgaa gcgatcgaac tggcgaaatc gttcggcgca gaagacagcc
    33961 ataagttcgt caacggcgta ctcgataaag cagcacctgt gattcgccct aacaaaaagt
    34021 aatatccagg ccggtagatt cacggaagac cgttccatga tcgccggcct tttctttttt
    34081 acctgctgag gcataacgta tggcatgtgg cgagttctcc ctgattgccc gttattttga
    34141 ccgtgtaaga agttctcgtc ttgatgtcga actgggcatc ggcgacgatt gcgctcttct
    34201 caatatcccc gagaaacaga ccctggcgat cagcactgat acgctggtgg cgggcaacca
    34261 cttcctccct gatatcgatc ctgctgatct ggcttataaa gcactggcgg tgaacctaag
    34321 cgatctggca gcgatggggg ccgatccggc ctggctgacg ctggcattaa ccttaccgga
    34381 cgtagacgaa gcgtggcttg agtccttcag cgacagcttg tttgatcttc tcaattatta
    34441 cgatatgcaa ctcattggcg gcgataccac gcgtgggcca ttatcaatga cgttgggtat
    34501 ccacggcttt gttccgatgg gacgagcctt aacgcgctct ggggcgaaac cgggtgactg
    34561 gatctatgtg accggtacac cgggcgatag cgctgccggg ctggcgattt tgcaaaaccg
    34621 tttgcaggtt gccgatgcta aagatgcgga ctacttgatc aaacgtcatc tccgtccgtc
    34681 gccgcgtatt ttacaggggc aggcactgcg cgatctggca aattcagcta tcgatctctc
    34741 tgacggtctg atttccgatc tcgggcatat cgtgaaagcc agcgactgcg gcgcacgtat
    34801 tgacctggca ttgctgccgt tttctgatgc gctttctcgc catgttgaac cggaacaggc
    34861 gctgcgttgg gcgctctctg gtggtgaaga ttacgagttg tgtttcacgg tgccggaact
    34921 gaaccgtggc gcgctggatg tggctctcgg acacctgggc gtaccgttta cctgtatcgg
    34981 gcaaatgacc gccgatatcg aagggctttg ttttattcgt gacggcgaac ctgtcacgtt
    35041 agactggaaa ggatatgacc attttgccac gccataaaga tgtcgcgaaa agtcgcctga
    35101 aaatgagtaa tccgtggcat ctgcttgctg tcggattcgg aagtggatta agcccgatcg
    35161 ttcctggcac gatggggtcg ctggcggcga ttcccttctg gtatctgatg acttttttgc
    35221 cctggcagct ctattcgctg gtggtaatgc tggggatctg tatcggcgtc tatctttgtc
    35281 atcaaacggc gaaagacatg ggcgtgcacg atcacggcag tattgtctgg gacgaattta
    35341 ttggtatgtg gattacgctc atggcgctgc cgaccaatga ctggcagtgg gttaccgccg
    35401 gatttgtgat tttccgtatt ctggatatgt ggaagccgtg gccgatccgc tggtttgatc
    35461 acaatgtaca tggcggcatg gggatcatga ttgacgatat tgtcgccggg gtgatctccg
    35521 ctggcatcct gtattttatc ggtcatcact ggccgctggg tattctgtcg tagttgtgca
    35581 ccgatgcctg atgtgacgct tgtcacgtct catcaggcct gcatacctat ttaaatccta
    35641 cgaccggatg cggtttatac ggcgtttcca gctcggcgat ctgttccggc ttcaaggtga
    35701 tatccaccgc gtttagcagt tcatcaagct gttcctcccg agaagttccg ataatcggtg
    35761 cggcaatgcc cggtttactc aacaaccagg ccagcgcaac ttgtgctcgc gttgcaccaa
    35821 gttcttcact gacgcccgtt aagcgttctg caatttgtgc gtcattttca ttgctttctt
    35881 ggtagagatt tttccccact tcatccgaca ccagccgtgc ggtggtttct ccccaaggac
    35941 gcgtcagacg gccccgcgcc agcggactcc atggaatcac cgccacgcct tcctgataac
    36001 acagcggcag catctcgcgc tcttcttcac gataaatcag attgtagtga tcctgcatac
    36061 tgacaaactg cgcccagccg tgctgttttt gtagttccag tgcctgagca aactgcgaag
    36121 cgtgcatgga tgacgcgccg atataacgtg ctttcccggc ttttaccacg tcgttcaggg
    36181 cttccagcgt ctcttcgatc ggcgtgttgt aatcccagcg atgaatttgc aggatatcga
    36241 cataatccat gccgagacgg agcaggctgt cgtcgataga gcgcaaaatt tgtgcacggg
    36301 ataatccttc cggtaaatca ccaacgcgat ggaacacttt ggtcgcaacg accacgtctt
    36361 cacgacgggc gaaatcccgc agtgcgcgac cgacgatctc ttcgctgctg ccatcggaat
    36421 agctattggc ggtatcaaag aaatttatgc cgccttccag tgcgcgttta atgatgggac
    36481 ggctgctctc ttccggcagt gtccatgcgt gattaccgcg atctggctcg ccaaaggtca
    36541 tacagccgag gcaaagtcgg gaaacacgaa ggtcggtttt tcctaagggg ttgtattgca
    36601 tgctgccact cctgccatga ataatttctt aagcatagca ggagtagagt agggattatg
    36661 ccagccaggc tttgattttg gcttccatac cggtggcatc gaggccgagt tcggcgcgca
    36721 tttcttcctg agttccttgt ggaataaaga agtccggcag accaatgttc agcacgggta
    36781 ctggtttacg atgggccatc agcacttcgt tcacgccgct gcctgcacca cccataatgg
    36841 cgttttcttc tacggtaacc agcgcttcat ggctggcggc catttccaga attaacgctt
    36901 catcaagcgg tttcacgaaa cgcatatcga ccagcgtagc gttcagcgat tcagcgactt
    36961 tcgccgcttc tggcatcagc gtaccaaagt taaggatcgc cagtttctca ccacgacgct
    37021 tcacaatgcc tttgccaatt ggcagttttt ccagtggcgt cagttccacg ccaactgcgt
    37081 tgccgcgcgg gtagcgcacc gcggacgggc catcgttata gtgatagccg gtatagagca
    37141 tctggcgaca ttcgttttca tcgctcgggg tcataatgac aatttccggt atgcagcgca
    37201 ggtaagagag atcaaaagcg ccctggtggg tttgaccgtc agcaccaaca atgcccgcac
    37261 ggtcgatggc gaacaggacc ggaagctttt gaatcgccac gtcatgcagc acctgatcat
    37321 aggcgcgttg caggaaggtg gagtagatcg caacaatggg tttgtagcct ccaatcgcca
    37381 gacccgcagc aaaggtcact gcgtgttgct cggcaattgc cacgtcgaag tagcgatccg
    37441 ggaatttacg tgaaaactcg accatgccgg aaccttcacg catcgccgga gtaatcgcca
    37501 tcagcttgtt atctttcgct gcggtttcgc acaaccagtc gccaaagatt tttgaatagc
    37561 ttggcaaacc gccgctactt ttcggcagac aaccgctgga gggatcaaat ttaggcacgg
    37621 cgtggaaggt gattgggtct ttttctgccg gttcataacc acgacctttt ttggtcatga
    37681 tatgcaggaa ctgcgggcct ttcaggtcgc gcatgttctt aagcgtggtg ataagcccca
    37741 gcacatcgtg accgtccacc gggccgatgt agttaaagcc cagctcttca aacaacgtgc
    37801 caggcactac catgccttta atatgttctt cggtacgttt gagcagctct ttaatgggtg
    37861 gcacgccaga gaaaactttt tttccgcctt cgcgcagtga agagtaaagc ttaccggaaa
    37921 gcagctgcgc cagatggttg ttgagcgcgc caacattttc ggaaatcgac atttcattgt
    37981 cgttgagaac caccagcata tcaggacgga tatcgcccgc gtgattcatc gcttcaaacg
    38041 ccatgccagc agtaatcgcg ccatcgccaa tgacacagac ggtgcggcga tttttgcctt
    38101 ctttctcggc agcaactgcg atcccaattc cggcactgat ggaggttgat gaatgcccga
    38161 cgcttaatac gtcatattcg ctttcgccgc gccacgggaa cgggtgcagg ccacctttct
    38221 gacggatggt gccgattttg tcgcggcgcc cggtcaaaat tttatgcgga taagcctgat
    38281 gccccacatc ccagattaac tggtcaaacg gggtattgta gacatagtgc agcgccacgg
    38341 tcagttcgac cgtgcccagc ccggaggcga agtgcccgct ggaacggctc acgctgtcga
    38401 gtaaatagcg gcgcagttcg tcgcagagtt tcggtaaact ctctttcggc aacagacgta
    38461 actcctgggt ggagtcgacc agtgccaggg tcgggtattt ggcaatatca aaactcatca
    38521 ggggcctatt aatacttatt gtttatttat tacgctggat gatgtagtcc gctagcgctt
    38581 ccagtgccga ggtatcgagt gactgttcag ccagttgttt cagcgactga cgggcatcgt
    38641 cgatcagatc ccgggctttc ttccgggctt gctcaagacc cagaagtgca gggtaggtac
    38701 ttttaccaag ttgctggtcg gcaccctggc gttttcccaa cgttgcagta tctcccacca
    38761 catccaggat gtcatcctga acctggaagg caaggccgat gctctctgcg tacttgtcga
    38821 gtacagacag agcacgacgt cctttatctc cggcgcttaa tgcaccaagg cgaacggcgg
    38881 cgcgaatcaa tgcgccggtt ttatgacgat gaatacgctc aagcgcctcc agaggtacgt
    38941 gtttgccttc cgcgtctaaa tctaatgcct gaccaccgca cattccggca ataccgctgg
    39001 cgctcgccag ttcagaaatc atcgaaattc tgtcgcgatc cgacacttcc ggcatatcag
    39061 catcgcttaa aatcgagaac gccagcgttt gtaaagcatc gccagcgaga atcgcgttcg
    39121 cttcgccaaa cttcacatgg caggtcggca aaccgcgacg cagatcgtca tcatccatcg
    39181 ccggtaaatc atcatgaatt aatgagtaag cgtggataca ctcaacggcg gcagcgggtg
    39241 cgtccagcgt gtttgtgcta acgccgaaca tatgaccggt ggcataaacc aggaaaggtc
    39301 gcaggcgctt accacctaat aatgcgccat actgcatggt ttcgaccacg ggagtgttct
    39361 gaaagggcag tggggcgata aaacggctca gcgcctggtt ggcctgctta acgcaggctt
    39421 cgagttgctg cggaaagtcc attactcatt gtccggtgta aaaggggtta gagaggcgtc
    39481 ttcattgtca gacagcagaa tttgcacgcg ctgttcggct tgttgtaatt tggcctgccc
    39541 ctgacgtgcc agttgcacgc cgcgttcgaa ctcgttcagc gcctcttcca gcggcaggtc
    39601 gccactttcc agacgggtta caatctgttc cagctcgctc agcgcctttt caaagctggc
    39661 gggcgcctca tttttcttcg gcataatgaa tgtctgactc tcaatatttt tcgccccgtc
    39721 atggtaacgg actcagggca aatagcaaat aacgcgcaat ggtaaggtga tgtgcgcagc
    39781 aaagcgatgt tagtggtata cttccgcgcc tggatgcagc cgcaggtgtg ggctgctgta
    39841 tttttcccta tacaagtcgc ttaaggcttg ccaacgaacc attgccgcca tgaagtttat
    39901 cattaaattg ttcccggaaa tcaccatcaa aagccaatct gtgcgcttgc gctttataaa
    39961 aatccttacc gggaacattc gtaacgtttt aaagcactat gatgagacgc tcgctgtcgt
    40021 ccgccactgg gataacatcg aagttcgcgc aaaagatgaa aaccagcgtc tggctattcg
    40081 cgacgccctg acccgtattc cgggcatcca ccatattctc gaagtcgaag acgtgccgtt
    40141 taccgacatg cacgatattt tcgagaaagc gttggttcag tatcgcgatc agctggaagg
    40201 caaaaccttc tgcgtgcgcg tgaagcgccg tggcaaacat gattttagct cgattgatgt
    40261 ggaacgttac gtcggcggcg gtttaaatca gcatattgag tccgcgcgcg tgaagctgac
    40321 caatccggat gtgactgtcc atctggaagt ggaagacgat cgtctcctgc tgattaaagg
    40381 tcgctacgaa ggtattggcg gtttcccgat tggtacccag gaagatgtgc tgtcgctcat
    40441 ttccggtggt tttgactccg gcgtttccag ttatatgttg atgcgtcgcg gatgtcgtgt
    40501 gcattactgc ttctttaacc ttggcggcgc ggcgcatgaa attggcgtgc gtcaggtggc
    40561 gcattatctg tggaaccgct ttggcagctc ccaccgcgtg cgttttgtcg ctattaattt
    40621 cgaacctgtc gtcggggaaa ttctcgagaa aatcgacgac ggtcagatgg gtgttatcct
    40681 caaacgtatg atggtgcgtg ccgcgtctaa agtggctgaa cgttacggtg tacaggcgct
    40741 ggtcactggc gaagcactgg gccaggtgtc cagccagacg ctgactaacc tgcgcctgat
    40801 tgataacgtc tccgacacgc tgatcctgcg tccgctgatt tcttacgaca aagagcacat
    40861 catcaacctg gctcgccaga ttggcactga agactttgcc cgcacgatgc ccgaatattg
    40921 cggtgtgatc tccaaaagcc cgacggtgaa agcggttaaa tcgaagattg aagcggaaga
    40981 agagaagttc gacttcagca ttctcgataa agtggttgag gaagcgaata acgttgatat
    41041 ccgcgaaatc gcccagcaga ccgagcagga agtggtggaa gtggaaaccg tcaatggctt
    41101 cggcccgaac gacgtgatcc tcgatatccg ttctatcgat gaacaggaag ataagccgct
    41161 gaaagtcgaa gggattgatg tggtttctct gccgttctat aaactgagca ccaaatttgg
    41221 cgatctcgac cagaacaaaa cctggctgtt gtggtgtgag cgcggggtga tgagccgcct
    41281 gcaggcgctc tatctgcgcg agcagggctt taacaatgtg aaggtatatc gcccgtaatt
    41341 tgtggttttt acgtcgcatc tggtcagatg cgacgtttgc cgcatccgcc actactcgta
    41401 ataattataa atccctgccg ccatcaccag ttgtgatgcc acttcatggg ctttttcacg
    41461 cccaaccagc aggtcgataa ttttcagacc aaagtcgata gctgtacccg gcccctgact
    41521 ggtcagcaat tttacccgtg catcccagac gacgcgcttg tcctgccatt gttcggcggg
    41581 tattttgtct ttcagcgtcg ggaagccggt catattgcca atcgggaaga tatcgtgcgg
    41641 gaccagcacg gtggctggtg cggcgcaaat agccgcgacg atacgcccgg aacggtggaa
    41701 ctgtttaacg gtttcaacca gcagggtgct atcgcgaaaa cactctgcgc ctttaatgcc
    41761 tccaggcagc acgatcacgt catattcgcc atcagctact tcgaccagcg gcgcatccgc
    41821 cagcagcttc acgccgcgcg agcaggtaat cgccaggttg ccatcgctgg cgacgctggc
    41881 agtggtgact ttgataccgc cgcgaaccag cagatcgata gtggtgacgg cttcagtctc
    41941 ttcactacca ggggcgaggc aaaccagtgc cgatgcgctc atattcactc tcctttcttt
    42001 ttaccatttc aaacaggcgg gtgttttccg gtacggtaat cccatgcgcg cgggcgcggc
    42061 gtaagagaaa accattgata tagtcgattt cggtgtggcg cagcgcgcgg atatcctgca
    42121 acatcgacga gatattttcc gctgtggcat caatcacctg catcacgtaa tcacgcaaat
    42181 cttctgctga agtatgatgt ccttcgcgtt cgatcactgc cgcgacttcc tcgcatatct
    42241 gcataatttc ttgcggatga tgacgtaatt caccgttcgg gcaattccag atggcagtca
    42301 gtggattaat cacacagttg actgccagct tgcgccacag ctcggcgcga atattgttat
    42361 gccaggcaac gtcaggcaac acggtttgca aaatatccgc cagataactg taatcgccat
    42421 cctgttgccg tgccggacca atatgcgtga taccgtttgc cacatgaata atgacattgc
    42481 cgtcgcggcg agcggcatgg gtggtggtgc ccatcagtaa tggctgctga atgttttgca
    42541 actcttcgat ggtgcccatg ccgttgtgaa ttaacagtat tggcgtagtt acaggcagtg
    42601 tggacgcgag gcttttgacg gcatcggaaa cctgccatgc tttcagcgtc accaggagca
    42661 gatcgctggt ggcgagaaaa tcgggatcgt tggcggtcag cgattcgtta aatatcgaac
    42721 catctgtctc aaccagattc acgctacaat aaggttgcgg tacgcgcagc cagccctgaa
    42781 cgtcgtgacc ctgtttacaa agtgctgtaa gccataattg ccctaaggca ccgcatccca
    42841 atacggtaat tttcattgtt cctcctcacc cgcaaccact ccgggtgttc aataaggcta
    42901 tcccttaatt gtgcatgctg ttgcgactat gcacaattaa gggatacgtc ctggtgcagg
    42961 actgttggtt atttaacttt gcaggtatta tgcttcgcat caaaaatgaa gggagaggaa
    43021 aagatgccat ctttcgatat tgtctctgaa gttgatcttc aggaagcacg taacgcggtc
    43081 gataacgcga gccgcgaagt ggagtcccgt tttgacttcc gtaatgttga agcctcattt
    43141 gagctgaacg acgccagcaa aaccatcaaa gtgttgagcg agtccgattt ccaggtcaat
    43201 cagttgctgg atattctgcg tgccaagctg ctgaagcgcg gcattgaagg cagttcgctg
    43261 gatgtaccgg aaaatatcgt tcatagcggt aaaacctggt ttgtggaagc gaaactgaaa
    43321 cagggcattg agagcgcgac tcagaagaaa atcgtcaaaa tgatcaaaga cagcaaactg
    43381 aaagtgcagg cgcaaattca gggcgatgag atccgcgtaa cgggtaaatc tcgtgatgat
    43441 ttgcaggctg tcatggcgat ggtacgtggt ggcgatctcg gtcagccgtt ccagttcaaa
    43501 aacttccgcg attaatcgcg acaacctgcg ctttgttcat gtcagatgcg gcatgaacgc
    43561 ctgatttggc ttacaaaggt ttgtaactca ggaacttagt aggcctgata agcgtagcgc
    43621 agcaggcaat ttcgcctcgc ttttatgcct ggcgaattgc ctgttcaacc tcaaagcgat
    43681 tcgtcacttt gctgtcgatt ttcacatacg ctgaatgttc ttcttctgca atcaacactt
    43741 ctttgacgcc ttcagtttcc agcaaacgta cttctaacgc ttcgtttgcg gcagtgtccg
    43801 ccgggatttc aatgcgcaag ctgctgacat acggcggttc tttcatggta ctggcgactg
    43861 ccagccacac tgcggccagc attgcgccag cgagaaatac tccctgaccg tcaaacatgc
    43921 cgtcaatcca gccgcccagc gaaccgccaa tcgccacgcc aagaaactgg ctggtggagt
    43981 aaacacccat cgccgtacct ttgtaacctg ctggcgattc tttactgata agtgaaggca
    44041 gaagggcttc catcaaatta aacgccacaa agaaaagctg cacgccgacc accagttgcc
    44101 agaactgcgt ttgtgcgttc cacaacacaa tttccgcaac cacgatcagc ccgacgcaaa
    44161 agacaaagac ttgcttcatt ttgcgcttaa cttcagcgta gataatgaaa ggcacgaccg
    44221 agccaaaggc gattaacatc gttgccaggt agaccttcca gtgttcagcc gccgggaacc
    44281 ccgcatcagc catctgtccg ggcagggcaa caaaggtcga catcagcaaa atatgcagac
    44341 acataatgcc aaagttgagt ttcagcagcc gcggttccgc cagcacttta ctgaaacttc
    44401 ctttcaccat tccggactca cgattaagta cgtgagtgct actgttgggc acaacccaaa
    44461 tggtcaacgc aatgccggtt gctgccagaa tagcgatcat ccagaacagc gcgtgcagcc
    44521 caagtttgtg agtgatgatc gggccaagca ccatcgcaat ggcaaaggta atgccaaagc
    44581 tcacgccgat aaacgccatc gctttggtgc ggttttgttc gcgcgtgaga tcggaaagca
    44641 gcgccataac ggcagcggca atcgcaccgg agccttgtag cgcccggccc agaataattc
    44701 cccagatgga gtcagagagc gcagcgataa cgctaccggc ggcaaacact gccagcccac
    44761 cgacaattaa tggtttgcga ccaatgcggt cggaaagcag gccaaacgga atctgaaaaa
    44821 cggcctgagt cagaccataa ataccaatgg caataccgat taatgcttcg ctggcacctt
    44881 gcagagccat gccgtacgtg gtcagaaccg gcagaaccat gaacatgccc agcatgcgca
    44941 acgagaatac ggtccctaaa ccccaggtcg cgcgtcgctc acctggcgtc attttataat
    45001 cgttcattac cacctctgtt ttaaattcgc gactagtgta aagcggcaaa cgaatggggt
    45061 aaacatgcgg attttttagt aaatatttcg agagggatat gattctaatg ctaagaaaaa
    45121 aggtgccgta gcaccttttt aactgagagg ttgtgttacc acacagcagc cagcagcgta
    45181 tgcgagtccg gcaccataaa atcaacggac atcatcaccg agagggcagt gatggcgatg
    45241 atagagaagc cgaacagctt gcgcgcccag attctgtcat cagcaacttt ataaccgcgc
    45301 agagccatgc ctaaccacca gacgctcacc gccgcggcga ctaccagata tttataccca
    45361 gcgtaaccgc caagagagag catcagcgtg gcaacggcaa aggcgatgat atacagcgtg
    45421 atgtgattct tcgccaccga aatgcctttt accactggca ataccggaat gtttgccgcc
    45481 tggtaatcct taaagcggaa aatggcgatg gcataggagt gaggcatctg ccacagactg
    45541 aagatagcaa gcaggatcgc tgcgccacta tcgaactcgc cggttactgc acagtagccg
    45601 atcaccggcg gcgcagcgcc ggagagcgaa ccaatcaatg tgccgtagac tgagtggcgt
    45661 ttcatgtaca ggctataaac gccgacataa accacaaagc ccatcacccc cagccagcag
    45721 gccagcggat tcgcgccaaa ccacagcagc ataaagccag caatacccag caaggtggcg
    45781 tacaccagcg agacagcagg tgagatcagg cctttaacca gcacccgatt cttcgtcctt
    45841 tccatctttc tgtcgatatc cctgtcgatg tagttgttaa acacacaacc cgacgccaca
    45901 accagtgaca ccccaaccag cgtgtagata aacaggggat aatcaatgct gccctttgag
    45961 gccaacagga atcccccaat caccgagatc aggttgccaa agatgatgcc tggtttcgtt
    46021 acttgcaggt attgcttaaa catcataacc gccgctctta gtgcatcatc atgttgtagt
    46081 tgaggttcca cataatccag atggagccta caaccaggat agcgatgatt agcacggtga
    46141 agacaaacgc cgtcatgttc cagccttcat ctgatttggt attcatgtgc aggaagcaca
    46201 ccagatgcac cagaacctgt accactgcca ttgccaggat tgttcccaga attacggccg
    46261 gagaggcagc tcctgtcatc accatccaga acggaatcac cgtcagaatg atcgacagga
    46321 taaagcctgt catgtaggtt tttacgctgc catgggacgc gccgctgtga tcggtagaat
    46381 gactcattac atcgccccca tcagataaac aacagtgaat acacagatcc aaaccacatc
    46441 caggaagtgc cagaacaggc tcaggcacat gatgcgggta cgattagtgc tggtcaggcc
    46501 gcgacgggcg atttgcacca tcagcaccgc catccagata agaccggaag tgacgtgcag
    46561 accgtgcgtg ccgaccagcg caaagaacgc tgacaggaag ccgctgcgat ccggacccat
    46621 gccgttaaca atcagatgat ggaattcata gagctccatc ccgataaatc cggcgccaaa
    46681 caaccaggtc aacgccagcc aggagacaac ctggcttttg ttgtttttgt acatggcgat
    46741 agccgccatg ccgtaggtga tggagctgaa caacagcagg aaagtttcaa ccagaacgaa
    46801 cggcagttcg aaaatgtcct tacctgtcgg gccgcctgcg gtgccgttca ccagaacggc
    46861 ataggtagca aacaggatag agaacagaat gcagtcgctc atcaggtaga tccagaatcc
    46921 gaagactttg gttccgcctg catcgtggtg cccgtgttcg tgcgcgtggg cagtcgcgtg
    46981 cgtcaaagta tcagttgcca tttttcagcc ctgccttagt aatctcatcg aaatgctggt
    47041 tttccagttt ttcgatttct gccaccggca cgtagtaatc cacgtcctcg tcgaagcttt
    47101 tcacgatcca ggtgatgatc atgcctgcga agccaacaat cgccagccac cagatatgcc
    47161 agatcatggc gaaaccgaag atggtggaga aagccgcaat gacgatacct gcaccgctgt
    47221 ttttcggcat atgaatttct tcatagtggt caggcttttt gtacgcttcg cctttctctt
    47281 tcatttccca gaatgcatca cgctcgtgaa cgtgcggcac aacggcaaag ttatagaacg
    47341 gaggcgggga agaggttgcc cactccagcg tacggccacc ccacgggtcg ccagtcaggt
    47401 cacggttctg gtcgcggtca cgaatagaaa cgtacatctg aataacgagg cagagaatac
    47461 ccagcgcaat cagtactgca ccgctggctg caatcatcag catggtgtgg aactgcgggt
    47521 caatctgctg gctcaaacga cgggtcatgc ccatgaagcc cagcgcatac agcggcataa
    47581 aggcaacgaa gaagccgatg atccagaacc agaacgcgcg tttaccccag gtttcgttca
    47641 gtttgaaacc gaacgcttta ggccaccagt aggtcatccc tgcgaagcag ccgaagacca
    47701 cgccgccgat gatcacgtta tggaagtgcg caatcaggaa caggctgtta tgcagaacga
    47761 agtccgcgcc cggtactgcc agcagcacgc cagtcatacc gcctaccgag aaggtgacga
    47821 taaaaccgat ggtccacagc atcgcagaat ggaacacgat gcggccctga tacatggtga
    47881 acagccagtt gaagatcttc accccggtcg ggatggcgat aatcatagtg gtgataccaa
    47941 agaaggcgtt tacgttcgcg cccgcaccca tcgtaaagaa gtggtgcagc cagacgatga
    48001 acgacagcac ggtgatacag acggttgccc ataccagcga ggtataacca aacagacgtt
    48061 tacgcgagaa ggttgccgca atttcggaga acacaccgaa aacaggcagg atcaggatgt
    48121 aaacttccgg gtggccccag gcccaaatca ggttgatgta catcatcatg ttgccaccca
    48181 tatcgttggt aaagaaatgg gtgcccagat agcgatccag ggtcaacaac gcaacggtaa
    48241 ccgtcagaat tgggaaggaa gcaataatca gtacgttcgc gcacagtgat gcccaggtaa
    48301 atactggcat cttgaacatg gtcatgcccg gtgcgcgcat cttcagaatg gtaacgaaga
    48361 agttgatacc ggtaagcgtt gtacctatac cggatagctg gagactccat atccagtaat
    48421 cgacaccgac tcccggactg tactctattc ccgatagcgg tggataggcc agccagccgg
    48481 tctgcgcaaa ttcgcccacg ccgagagaaa cgttaaccag aatcacacca acaacggtaa
    48541 accagaagct taggttgttg aggaacggga acgcaacgtc acgcgcgccg atctgcagcg
    48601 gaaccaccag gttcatcaga ccgataacga aaggcatcgc cacgaagaag atcataatca
    48661 cgccgtgcgc ggtgaagatc tgatcgtagt ggtgaggtgg caggaagccc gcttcgcccg
    48721 ccgaggcaag agcctgctgg ctacgcatca taatggcgtc agcaaaacca cgcagcaaca
    48781 tcacaatcgc cacgatgata tacatgatac cgaggcgttt atggtcgacg gaggtcagcc
    48841 actctttcca caggtaggtc cacttaccga agtaagtgat caggccaacg agcgccagac
    48901 ctcccaaaat aatgccagcg atcgtaacca tgacgatagg ttcatggaac gggactgcat
    48961 caagtgataa ttttccgaac atctttattc ttcctcaacc cctttaatgg gcggattccg
    49021 cgtggctcat gtccatgcct tccatacctt catgtgcgct gtgctcacct tctggctggg
    49081 tcatgtccat gctcttaccg tgagccataa acttgttaat cacatcggca aacaagtctg
    49141 gtttcacgtt ggagaaatat tccacctggt tgtattcgct aggcgcggcc agtttttcga
    49201 acgctgccat gtcagacatg ctgttcggcg actgtttcgc ttttgcgacc cattggtcga
    49261 atgcggcgcg atccggtgtt gcaatagctt tgaacttcat gcctgagaag cccgggccgc
    49321 tgtagctggc ggagataccg tcataagtac cgggttcgtt ggcgatcaga tgcaggcgag
    49381 tctgcatacc ggccatggca taaatctggc tacccagacg cggaatgaag aaggagttca
    49441 tcacggagtt ggaggtcact ttgaagtaca ccggagtgtt cgccgggaaa gcgatttcat
    49501 tcacggttgc aatgccctgt tccgggtaga tgaagaacca tttccagtcc atggaaacca
    49561 cttcgatggt aatgggcttc tcgtcgtgtg ccagcggctt gctaggctca agagcgtgag
    49621 tggttttcca ggtcagtacc gcaaggaaga tgatgattaa gataggtacc gtccagacca
    49681 cagcttccac tttattggag tgtgaccagt tcgggctgta cttagcatct ttattgctcg
    49741 cacggtactt ccaggcgaaa ccaacagcca tcaagattgc gggaataacg acaatcaaca
    49801 tcaggccaaa tgccgtcagt atcagtgaac gttgctccag accaatctgt cctttgggat
    49861 ctaacagcgc agaattacag ccactgagca atacagtgcc tgcaaataat gacaaccatc
    49921 ccaaactttt attgtatttc ctgagtctca tttaacgacc tcaattccac gggatctggt
    49981 ggcgtttaaa gtgtgtgggc attttacggg aaggttacat tactgtaaac atgattaaat
    50041 tcctgttact tggatttggc ttacaggtca catatgttgc aaaacatatc aggttgattt
    50101 taagaagctg ttgcaaaagg gcgttataac aaaaggggag tgcagaagga gtctgggcgg
    50161 gcaattggta taaccaatgc gaaataaaac aattatttaa caaataatta tcattaaggt
    50221 gacaactggg tagtgggaaa ataaagactc attagctgaa tcgtgtaaga aaaatagaat
    50281 tcataaatgg cgtcaataat ttccagacgc taagccgcac aaaagaacaa atattaataa
    50341 gcgatgctga tgtttattta gccgtaataa ttacggcgag tgattactac ggctaaataa
    50401 tatttacaga ttacgtcaga tgcgtttttc gtagcgccag ataatccagt aaaccaccaa
    50461 gcacgacgcc agaaagggcg actaaaaccc cgacttccag cagagcaggc aggaaagaga
    50521 agtgcgtcaa atccagcgcg tccatcgtca acagcagtaa ccacacggcc aacaggctga
    50581 cgcccgccgc cagtgtccac atggcaaagg cataacctgc cggatattcg gtacgggaga
    50641 taaagttatc atttactcgt gtatattcaa gcgtctggcg acaaaccagc agcaagataa
    50701 gccctggtac ggcagcggcg acggagaata gatagaatgt cgaccagccg tgtgcttcaa
    50761 caaaccaacc cgccacgggg ccgacataaa ctcgccctac agcagaaagc gctgagagca
    50821 gggcaaattg agtagcggaa aatgacttat tacacagcgt cattaacagc gcgacaaagg
    50881 ctgatgtacc catcccgcca cagaggtttt cgaaaaagac ggctgcgccc atgctgtaga
    50941 gatgcttatc agtaatcgac agcagccagt aaccggcgtt agatgcacct tgtaaaatgc
    51001 cgaaaatcag cagtgcacgg aacagcgaca ggcgctgcat caaaatccca ccgtacaatg
    51061 cgccaacaat ggtcgccagt aagccaagcg ttttgttaac cacgcctact tcacccgcat
    51121 caaacccgac gccgcgaatc aaaaacgtgg ttgtcaggct catagcgaat gcgtcgccca
    51181 gcttatacag cacgataaga agcaaaataa gccaggcatt attgcgacca aagaaatctc
    51241 gcagaggtgc aacaaccgct tgttccagcg ttttgggcac aggaatggtg tcggtgggtt
    51301 ctggtgcaag caacgtcgcg ataatacagg ggatcaacag cgccgccatc aaccagtaca
    51361 tgccctgcca gcccagccat ttatctgcca gccacagggc taagccgccg gaaaccagca
    51421 tccctaaacg gtaacccagc acgctgattg ccgcgcccgc accacgttct tctgccggga
    51481 gcacatcggt tttccacgca tcgaagacaa tatcctggga ggcagagcaa aaagcgatca
    51541 ccaccgccag tgccgccatc cagcggagct gggtgcctgg ttcgagaaaa cccatcgccg
    51601 caatggcgac taataacaga atttgcgtgg cgagcagcca accgcgccgc cgcccgaaaa
    51661 atgggggcgt gtagcggtcc atcagtggtg accagaggaa tttaaaaacg taagcctggc
    51721 ctaccagaga gaagaaacca atggttttga gatcgatatt ctcgaccgtc atccaggcct
    51781 gtaaggtgcc ggacgtcagg gcgagcggta gcccggaagc aaaacccagg atcagcaata
    51841 tggctgaacg cggctgttga aaaatacgta aatattgact ggacatgggc gtctacaggc
    51901 ccggcttgca ccgggccaga gggcagaaat taacgcgcgt tctgcttgat gaattcgtgg
    51961 atgctggtgt cctgagacat atcagcgatg gtatcggtca gcacactgtt aaccgcatcg
    52021 gcgatatttt tgttggaggc ctggaacgca ccttcaacgt tgtagctggc acgatagttt
    52081 ttggtcattt tgttgccatt ctgcgcggta gcgatgatgg cgatatccgc tttggtcgcg
    52141 atgttgtagc gcacgttgcc ctgggacacg tcagcataca gttggctaac gatgatttgc
    52201 agattaaccg ggccattcgg accaaccatg tatccacggg cggtcatttg tttttccagc
    52261 acttcttgca gcaggaaacg cagatcgcgg gaggcggtca gggtaacgat ttgattatcg
    52321 cgggtgactt ttgccagcgc ctgatcggta cgctgatcgg caccattaat gcttacggtg
    52381 acgcccatca ggcttggatc ctgctgtggc agtgtaatcg tcggggaaac ttcaatagtt
    52441 gttggcggtt ttgcgcatcc tgcaagcata aacagagcaa ctaacgggaa gaggattttt
    52501 ttgaacatgt tcgggctctc agagactctt aagcgtgttt ggtaaaaatt cccgccatca
    52561 taacattgcc aacggcgagg ggaagtgggt aaggcatgta aattcatcat gttgacgaaa
    52621 taatcgcccc tggtaaaaga aacgctgatg cgaggcctgt gtttcaatct ttaaatcagt
    52681 aaacttcata cgcttgacag aaaaaccagg acgaaaccta aatatttgtt gttaagctgc
    52741 aatggaaacg gtaaaagcgg ctagtattta aagggatgga tgacatctca gcgttgtcgg
    52801 aggagatatt tcatgatgat acgtgagcgg atagaagaaa aattaagggc ggcgttccaa
    52861 cccgtattcc tcgaagtagt ggatgaaagc tatcgtcaca atgtcccagc cggctctgaa
    52921 agccatttta aagttgtgct ggtcagcgat cgttttacgg gtgaacgttt tctgaatcgt
    52981 catcgaatga tttacagtac tttagcggag gaactctcta ctaccgttca tgcgctggct
    53041 ctgcatactt acactattaa ggagtgggaa gggttgcagg acaccgtctt tgcctctcct
    53101 ccctgtcgtg gagcaggaag catcgcgtaa aaacgcattt gcaactgtcg gcgcttttcc
    53161 agtatgttgc taaagatttt atgaaaaacg gcctgcgggc cgttttgttt tgtctggatt
    53221 ttgcgctttt tgcccagcat tcagacgaaa attgcccggg aattgtgaaa aaatacgcga
    53281 cagcgcgcaa taaccgttct cgactcataa aagtgatgcc gctataatgc cgcgtcctat
    53341 ttgaatgctt tcgggatgat tctggtaaca gggaatgtga ttgattataa gaacatcccg
    53401 gttccgcgaa gccaacaacc tgtgcttgcg gggtaagagt tgaccgagca ctgtgatttt
    53461 ttgaggtaac aagatgcaag tttcagttga aaccactcaa ggccttggcc gccgtgtaac
    53521 gattactatc gctgctgaca gcatcgagac cgctgttaaa agcgagctgg tcaacgttgc
    53581 gaaaaaagta cgtattgacg gcttccgcaa aggcaaagtg ccaatgaata tcgttgctca
    53641 gcgttatggt gcgtctgtac gccaggacgt tctgggtgac ctgatgagcc gtaacttcat
    53701 tgacgccatc attaaagaaa aaatcaatcc ggctggcgca ccgacttatg ttccgggcga
    53761 atacaagctg ggtgaagact tcacctactc tgtagagttt gaagtttatc cggaagttga
    53821 actgcagggt ctggaagcga tcgaagttga aaaaccgatc gttgaagtga ccgacgctga
    53881 cgttgacggc atgctggata ctctgcgtaa acagcaggcg acctggaaag aaaaagacgg
    53941 cgctgttgaa gcagaagacc gcgtaaccat cgacttcacc ggttctgtag acggcgaaga
    54001 gttcgaaggc ggtaaagcgt ctgatttcgt actggcgatg ggccaggggc gtatgattcc
    54061 gggctttgaa gacggtatca aaggccacaa agctggcgaa gagttcacca tcgacgtgac
    54121 cttcccggaa gaataccacg cagaaaacct gaaaggtaaa gcagcgaaat tcgctatcaa
    54181 cctgaagaaa gttgaagagc gtgaactgcc ggaactgact gcagaattca tcaaacgttt
    54241 cggcgttgaa gatggttccg tagaaggtct gcgcgctgaa gtgcgtaaaa acatggagcg
    54301 cgagctgaag agcgccatcc gtaaccgcgt taagtctcag gcgatcgaag gtctggtaaa
    54361 agctaacgac atcgacgtac cggctgcgct gatcgacagc gaaatcgacg ttctgcgtcg
    54421 ccaggctgca cagcgtttcg gtggcaacga aaaacaagct ctggaactgc cgcgtgaact
    54481 gtttgaagaa caggctaaac gtcgcgtagt tgttggcctg ctgctgggcg aagttatccg
    54541 caccaacgag ctgaaagctg acgaagagcg cgtgaaaggc ctgatcgaag agatggcttc
    54601 tgcgtacgaa gatccgaaag aagttatcga gttctacagc aaaaacaaag aactgatgga
    54661 caacatgcgc aatgttgctc tggaagaaca ggctgttgaa gctgtactgg cgaaagcgaa
    54721 agtgactgaa aaagaaacca ctttcaacga gctgatgaac cagcaggcgt aatttacgca
    54781 gcataacgcg ctaaattcgc acaaaggccc gtcaccgcca ggtggtgggc ttttttttgt
    54841 catgaatttt gcatggaacc gtgcgaaaag cctctttcgg tgttagcgta acaacaaaag
    54901 attgttatgc ttgaaatatg gtgatgccgt acccataaca cagggactag ctgataatcc
    54961 gtccataagg ttacaatcgg tacagcaggt tttttcaatt ttatccagga gacggaaatg
    55021 tcatacagcg gcgaacgaga taactttgca ccccatatgg cgctggtgcc gatggtcatt
    55081 gaacagacct cacgtggtga gcgctctttt gatatctatt ctcgtctact taaggaacgc
    55141 gtcatttttc tgactggcca ggttgaagac cacatggcta acctgattgt ggcgcagatg
    55201 ctgttcctgg aagcagaaaa cccagaaaaa gatatctatc tgtacattaa ctccccaggc
    55261 ggggtgatca ctgccgggat gtctatctat gacaccatgc agtttatcaa gcctgatgtc
    55321 agcaccatct gtatgggcca ggcggcttcg atgggcgctt tcttgctgac tgcaggggca
    55381 aaaggtaaac gtttctgcct gccgaattcg cgcgtgatga ttcaccagcc gttgggcggc
    55441 taccagggcc aggcgaccga tatcgaaatt catgcccgtg aaattctgaa agttaaaggg
    55501 cgcatgaatg aacttatggc gcttcatacg ggtcaatcat tagaacagat tgaacgtgat
    55561 accgagcgcg atcgcttcct ttccgcccct gaagcggtgg aatacggtct ggtcgattcg
    55621 attctgaccc atcgtaattg atgccagagg cgcaactgtg ccgctatact tatccagggc
    55681 ggcacaacgc tgtaagcggc ttgcgcctga gaatggcatt tgcgtcgtcg tgtgcggcac
    55741 aaagaacaaa gaagaggttt tgacccatga cagataaacg caaagatggc tcaggcaaat
    55801 tgctgtattg ctctttttgc ggcaaaagcc agcatgaagt gcgcaagctg attgccggtc
    55861 catccgtgta tatctgcgac gaatgtgttg atttatgtaa cgacatcatt cgcgaagaga
    55921 ttaaagaagt tgcaccgcat cgtgaacgca gtgcgctacc gacgccgcat gaaattcgca
    55981 accacctgga cgattacgtt atcggccagg aacaggcgaa aaaagtgctg gcggtcgcgg
    56041 tatacaacca ctacaaacgt ctgcgcaacg gcgataccag caatggtgtc gagttgggca
    56101 aaagtaacat tctgctgatc ggtccgaccg gttccggtaa aacgctgctg gctgaaacgc
    56161 tggcgcgcct gctggacgtc ccgttcacca tggccgacgc aaccacgctg accgaagccg
    56221 gttatgtggg cgaagacgtt gaaaacatca ttcagaagct gttgcagaag tgcgattacg
    56281 acgtacagaa agcgcagcgc gggattgtct acatcgatga aatcgacaag atttctcgta
    56341 agtcagacaa cccgtccatt acccgagacg tttccggtga aggcgtacag caggcactgt
    56401 tgaaactgat cgaaggtacg gtagctgctg ttccaccgca aggtgggcgt aaacatccgc
    56461 agcaggaatt cttgcaggtt gatacctcta agatcctgtt tatttgtggc ggtgcgtttg
    56521 ccggtctgga taaagtgatt tcccatcgtg tagaaaccgg ctccggcatt ggttttggcg
    56581 cgacggtaaa agcgaagtcc gacaaagcaa gcgaaggcga gctgctggcg caggttgaac
    56641 cggaagatct gatcaagttt ggtcttatcc ctgagtttat tggtcgtctg ccggttgtcg
    56701 caacgttgaa tgaactgagc gaagaagctc tgattcagat cctcaaagag ccgaaaaacg
    56761 ccctgaccaa gcagtatcag gcgctgttta atctggaagg cgtggatctg gaattccgtg
    56821 acgaggcgct ggatgctatc gctaagaaag cgatggcgcg taaaaccggt gcccgtggcc
    56881 tgcgttccat cgtagaagcc gcactgctcg ataccatgta cgatctgccg tccatggaag
    56941 acgtcgaaaa agtggttatc gacgaatcgg taattgatgg tcaaagcaag ccgttgctga
    57001 tttatggcaa gccggaagcg caacaggcat ctggtgaata attaaccatt cccatacaat
    57061 tagttaacca aaaagggggg attttatctc ccctttaatt tttcctctat tctcggcgtt
    57121 gaatgtgggg gaaacatccc catatactga cgtacatgtt aatagatggc gtgaagcaca
    57181 gtcgtgtcat ctgattacct ggcggaaatt aaactaagag agagctctat gaatcctgag
    57241 cgttctgaac gcattgaaat ccccgtattg ccgctgcgcg atgtggtggt ttatccgcac
    57301 atggtcatcc ccttatttgt cgggcgggaa aaatctatcc gttgtctgga agcggcgatg
    57361 gaccatgata aaaaaattat gctggtcgcg cagaaagaag cttcaacgga tgagccgggt
    57421 gtaaacgatc ttttcaccgt cgggaccgtg gcctctatat tgcagatgct gaaactgcct
    57481 gacggcaccg tcaaagtgct ggtcgagggg ttacagcgcg cgcgtatttc tgcgctctct
    57541 gacaatggcg aacacttttc tgcgaaggcg gagtatctgg agtcgccgac cattgatgag
    57601 cgggaacagg aagtgctggt gcgtactgca atcagccagt tcgaaggcta catcaagctg
    57661 aacaaaaaaa tcccaccaga agtgctgacg tcgctgaata gcatcgacga cccggcgcgt
    57721 ctggcggata ccattgctgc acatatgccg ctgaaactgg ctgacaaaca gtccgttctg
    57781 gagatgtccg acgttaacga acgtctggaa tatctgatgg caatgatgga atcggaaatc
    57841 gatctgctgc aggttgagaa acgcattcgc aaccgcgtta aaaagcagat ggagaaatcc
    57901 cagcgtgagt actatctgaa cgagcaaatg aaagctattc agaaagaact cggtgaaatg
    57961 gacgacgcgc cggacgaaaa cgaagccctg aagcgcaaaa ttgacgcggc gaaaatgccg
    58021 aaagaggcaa aagagaaagc ggaagcagag ttgcagaagc tgaaaatgat gtctccgatg
    58081 tcggcagaag cgaccgtagt gcgtggttat atcgactgga tggtacaggt gccgtggaat
    58141 gcgcgcagca aggtcaaaaa agacctgcgt caggcgcagg aaatccttga taccgaccat
    58201 tatggtctgg agcgcgtgaa agatcgcatc cttgagtatc ttgcggttca aagccgtgtc
    58261 aacaaaatca agggaccgat cctctgcctg gtagggccgc cgggggtagg taaaacctct
    58321 cttggtcagt ccattgccaa agccaccggg cgtaaatatg tccgtatggc gctgggcggc
    58381 gtgcgtgatg aagcggaaat ccgtggtcac cgccgtactt acatcggttc tatgccgggt
    58441 aaactgatcc agaaaatggc gaaagtgggc gtgaaaaacc cgctgttcct gctcgatgag
    58501 atcgacaaaa tgtcttctga catgcgtggc gatccggcct ccgcactgct tgaagtgctg
    58561 gatccagagc agaacgtagc gttcagcgac cactacctgg aagtggatta cgaccttagc
    58621 gacgtgatgt ttgtcgcgac gtcgaactcc atgaacattc cggcaccgct gctggatcgt
    58681 atggaagtga ttcgcctctc cggttatacc gaagatgaaa aactgaacat cgccaaacgt
    58741 cacctgctgc cgaagcagat tgaacgtaat gcactgaaaa aaggtgagct gaccgtcgac
    58801 gatagcgcca ttatcggcat tattcgttac tacacccgtg aggcgggcgt gcgtggtctg
    58861 gagcgtgaaa tctccaaact gtgtcgcaaa gcggttaagc agttactgct cgataagtca
    58921 ttaaaacata tcgaaattaa cggcgataac ctgcatgact atctcggtgt tcagcgcttc
    58981 gactatggtc gcgcggataa cgaaaaccgt gtcggtcagg taactggtct ggcgtggacg
    59041 gaagtgggcg gtgacttgct gaccattgaa accgcgtgcg ttccgggtaa aggcaaactg
    59101 acctataccg gatcgcttgg cgaagtgatg caggagtcca ttcaggcggc gttaacggtg
    59161 gttcgtgcgc gtgcggaaaa actggggatc aaccctgatt tttatgaaaa acgcgacatc
    59221 cacgtccacg taccggaagg tgcgacgccg aaagatggtc cgagtgccgg tattgctatg
    59281 tgcaccgcgc tggtttcttg cctgaccggt aacccggttc gtgctgatgt ggcaatgacc
    59341 ggtgagatca ctctgcgtgg tcaggtactg cctatcggtg gtttgaaaga aaaactactg
    59401 gcagcgcatc gcggcgggat caaaacagtg ttaattccgt tcgaaaataa acgcgatctg
    59461 gaagagattc ctgacaacgt aattgccgat ctggatattc atcctgtgaa acgcattgag
    59521 gaagttctga ctctggcgct gcaaaatgaa ccgtctggca tgcaggttgt gactgcaaaa
    59581 tagtgacctc gcgcaaaatg cactaataaa aacagggctg gcaggctaat tcggacttgc
    59641 cagccttttt ttgtctcgct aagttagatg gcggattggg cttgccctta ttaaggggtg
    59701 ttgtaagggg atggctggcc tgatataact gctgcgcgtt cgtaccttga aggattcaag
    59761 tgcgatataa attataaaga ggaagagaag agtgaataaa tctcaattga tcgacaagat
    59821 tgctgcaggg gctgatatct ctaaagctgc ggctggccgt gcgttagatg ctattattgc
    59881 ttccgtaact gaatctctga aagaagggga tgatgtagca ctggtaggtt ttggtacttt
    59941 tgccgttaaa gagcgtgctg cccgtactgg ccgcaatccg cagaccggta aagagatcac
    60001 catcgctgct gctaaagtac cgagcttccg tgcaggtaaa gcactgaaag acgcggtaaa
    60061 ctaagcgttg tccccagtgg ggatgtgacg aagttcaagg gcgcatctac tgatgtgcct
    60121 tttttatttg tattcggtga ctttctgcgt cttgtgggct gacaattgcc cccgtttctt
    60181 gtcacaatat gcctttgcgc gcatcgatac gttgcgtgag gtacacagtc atctacagcg
    60241 gagtgttgtt acaccatgat ggacagctta cgcacggctg caaacagtct cgtgctcaag
    60301 attattttcg gtatcattat cgtgtcgttc atattgaccg gcgtgagtgg ttacctgatt
    60361 ggcggaggca ataactacgc cgcaaaagtg aatgaccagg aaatcagtcg tgggcaattc
    60421 gagaacgcct tcaacagcga gcgtaatcgc atgcagcaac agctgggcga tcaatactcc
    60481 gagctggcag cgaacgaagg ctatatgaaa accctgcgtc aacaggtgct gaatcgtctg
    60541 atcgacgagg cgctgctgga tcagtacgct cgtgagctga aactgggtat cagcgatgag
    60601 caggttaaac aggcgatttt cgcgacccca gccttccagg ttgacggcaa atttgataac
    60661 agccgctata acggtatcct caaccagatg gggatgaccg ccgatcagta cgcccaggcg
    60721 ctgcgtaacc agctcactac ccaacagctg attaacggcg ttgccggtac cgattttatg
    60781 ctgaaaggtg aaaccgacga gctggcggca ctggtagttc aacaacgcgt ggtgcgtgaa
    60841 gcgactatcg atgttaacgc gctggcggcg aagcagcctg tgaccgaaca ggaaattgcc
    60901 agctactacg aacaaaacaa aaacaatttc atgacgccgg aacaattccg cgtgagttac
    60961 atcaagctgg atgccgcaac gatgcagcaa ccggttagcg atgcggatat ccagagctac
    61021 tacgatcagc atcaggatca attcacccag ccgcagcgta cccgttacag catcatccag
    61081 accaaaactg aagatgaagc gaaagcggta cttgatgagc tgaataaggg cggtgatttt
    61141 gctgcattag ccaaagaaaa atctgccgat attatctctg cccgcaacgg cggtgatatg
    61201 ggttggttag aagatgccac tatcccggac gaactgaaaa atgctggtct gaaaaaaaaa
    61261 ggccaactgt ctggtgttat caaatcttcg gtcggtttcc tgattgtgcg tctggacgac
    61321 attcagccag cgaaagtgaa atcgttagac gaagtacgtg acgacattgc ggcgaaagtg
    61381 aaacacgaaa aagccctcga tgcgtactac gcgctgcagc agaaagtgag cgatgcggca
    61441 agcaacgaca ccgagtctct ggccggtgca gagcaagctg ccggcgttaa agccactcag
    61501 acgggttggt tcagcaaaga taacctgccg gaagagttga acttcaagcc agttgccgac
    61561 gctatcttca acggcggtct ggtcggtgaa aacggcgcgc cgggcatcaa ctctgacatc
    61621 atcaccgtag acggcgaccg cgcattcgtg ctgcgcatca gcgagcacaa accggaagcg
    61681 gtgaaaccgt tggcagatgt tcaggaacaa gttaaggcac tggttcagca caacaaagct
    61741 gaacaacagg cgaaagtgga tgctgagaaa ctgctggttg atttgaaagc aggcaaaggt
    61801 gcggaagcta tgcaggctgc cggtctgaaa tttggcgagc cgaaaacctt aagtcgttcc
    61861 ggtcgtgacc cgattagcca ggctgcgttt gcactgccgc tgccagcgaa agacaaaccg
    61921 agctacggta tggcgaccga tatgcaaggt aatgtagttc tgctggcgct ggatgaagtg
    61981 aagcaaggtt caatgccgga agatcagaaa aaagcgatgg tgcagggtat cacccagaac
    62041 aacgcacaaa tcgtctttga agctctgatg agtaacctgc gtaaagaggc gaaaatcaaa
    62101 attggcgatg cgctggaaca gcaataatcc tgaagccgcc tcgcaaaaaa atgctttgca
    62161 gctgtaacaa gaaaaggtcg ctttcgcggc cttttccatt tctgaacatt gccatttgtt
    62221 tactgttttc actgccgtta aggtgattcc actgttaaca aacaaggaga aaacagtatg
    62281 aaacacggaa ttaaagcact gctcattacc ctgtccctgg cctgtgccgg aatgtctcat
    62341 agtgctctgg cggcagctcc tgcggcaaaa ccgacaacgg tagaaaccaa agcggaagct
    62401 cctgcagcac aaagtaaagc agcagttccg gcgaaagcca gtgacgaaga aggcacccgg
    62461 gtcagcatta ataatgccag cgcggaagag ttagcccgcg cgatgaatgg cgttggcctg
    62521 aagaaagcgc aggcgattgt cagttatcgc gaagagtacg gtccgtttaa aacggtggag
    62581 gatctaaagc aggtgccggg gatgggcaat tcgctggtgg agcgtaatct ggcggtatta
    62641 accctgtaat taatttgcat agtggcaatt ttttgccaga ctgaagaggt cataccagtt
    62701 atgacctctg tacttataac aacaacgtaa ggttctcgcg ctatgcaaac acaaatcaaa
    62761 gttcgtggat atcatctcga tgtttaccag cacgttaata acgcccgcta tctggagttt
    62821 ctcgaagaag cccgctggga tgggttggaa aatagcgaca gttttcagtg gatgacggcc
    62881 cataacatcg ctttcgtggt ggtgaatatc aatattaact atcgtcgccc ggcggtatta
    62941 agcgacttgt taaccattac gagccagtta cagcaattaa acggtaaaag cggcatctta
    63001 agccaggtca ttacactgga gccggaaggg caggtggtag cggatgcgct tattacgttt
    63061 gtttgtattg atcttaaaac gcagaaagca ttagctctgg aaggggaatt gcgcgaaaag
    63121 ctcgagcaga tggttaagta aacgttttgt ggtgccggat gctcaagccg catccggcga
    63181 cacccggaat aattacttca acccggtttt ctgcttcatc gctgccatca ccgtcggttt
    63241 atcggccaga taatgatgca aaccgttggc acgtaaatta cacgccgcac aatgaccgca
    63301 accgtcgcct ttaatgccgt tataacaggt cagcgtttcg ttacggacta aatccagctt
    63361 gccgtaataa tctgccagcg cccaggtttc cgctttatca atccacatca gcggcgtttc
    63421 aaaacgaatg tctttcgcca tacccagact gacggcatgg ttcagtgctt tcacaaactc
    63481 atcgcggcaa tccgggtagc cggagaaatc cgtttcgcag acgccggtaa tcaccgcttc
    63541 tgcttttacc tgatacgcat atattgccgc cagtgtcagg aacaaaatat tacgcccagg
    63601 gacaaacgta ttcgggatgc catcggcttc aggttcataa tctggcactg gaatgctgtc
    63661 acgtgtcagg ctgctgaccg ccagctcgtt gagcagcgtt acatcaagaa ccttatgcgc
    63721 gcgtgccccc agtttcagcg ccagttcgcg tgccacgtcg atttccgcgc gatgtcgctg
    63781 accgtaatcg aacgtcacgc aatggacttc atcatattgt tgtaatgcct gcaccagaca
    63841 ggtggtagaa tcctggcctc cactgaacac aacgacagca cgtttcatag ataatcctgc
    63901 ttgaacaata aaagcgttat ggtaacgcct gcgatgaacc cggaccagct attcattgcg
    63961 atggcgcggg aagccaggct tcggtaaatt caaaccagcc tcgcggtgtc agtcgcacac
    64021 cgttcacgcc gggaggggcg ctaatgcgat agtgatagtt gaacagcggc gtcagcgtcg
    64081 catctgccat taactggcta aaaaccgctt tcagggcatt aaatcggttt tcctcatcag
    64141 gcattacttg caccgcgtcc agtgtcgatt gcaaatgtgc atatgctgga gcgtcgaaaa
    64201 catgtggcca tagtggatcg caacgcagcc attgctccag ggtatattcc ggtgcttcgc
    64261 caattaatct gtcgcccatc atgaggtctg cgtgtgccag tagggtcgtg tcgtcccagt
    64321 tttttgcgtt atgaaaaata attgtgagtt cacagccttc cgctgccagt gtcgcctgta
    64381 gacgttctgc catggtatga agttctatcg gtaggtgata aaccagcgtc aaggttttcg
    64441 gtagtttgac ctcatccggt acttgccatt gcggaatagt ccagcctggc agtaatgcat
    64501 gactggcggt gatcaggttt tctccgactt ctaacgtttg taataaaccg gattgatgaa
    64561 taatggaaat cacttttcgc gcctgccaga gggacagtcg gggacttttg cgcaacgtta
    64621 aatagcaaaa acctaaactg atgccactac tgacctggct gacccgttgc agctcctccg
    64681 gtttgccgat ggtgatttgc acgggatgcc gacaactggt tcccaaatct ttttcgaaaa
    64741 gcggcggagt tatccagtac tcaaccgctt taagcagcgg atgacgtaaa tggtaataat
    64801 catggctttc caggcgcacc agctccgctg tgaattgtgt taagcgaaaa ggacccgtgc
    64861 cgatcagtgg gaattgcgga tgcgccagat ggctgcaata gctcgccagt cggtgcgcaa
    64921 gccagtaatc ggggcgatgt aaaaagaagg tcagacactg cggatgggtg acttcaatac
    64981 gcttcacgct aataaataat tgatcccgtg ctggcagttg taacagcagc aataatcgct
    65041 ggtgtaagtg tgaggctttt actgcatcgc cgttatgcca gtgtagggtt gaacgaagat
    65101 aaaagtccca gcgtaacccg tcagtagagg tttcccagtg atgtgctaaa tcgccaatcg
    65161 ggcgctgagt attattatcg aagcgggtca ggccggaaaa tatctgcccg gccagatgct
    65221 gctcggcacg gccgggcaaa aagcccggtt gtagcggttc gagcgggcga tagtagggaa
    65281 tacgcaacgt gggcgtatcg ttttgccatt gtccgcccat aaacggctgt aacagagtgc
    65341 gcagctcacc tggggccagt tgcgccagct ccagcacatc ttgctgcttt ccggtttcca
    65401 gcgcctgttc catcatcgca ttgcgtagcg attctggcgt gaccagaaag cgtagttgcc
    65461 cgcgttttcc gcgtcctgac tgcgcctgcc actccagcca tcccgcctcc tgtgcctggc
    65521 gtaacagcgt acgaacatgg cgttcgctgc aaaaacagcg ttcggccagt tcgctgacgg
    65581 tgacggtttg cggctttccg gcggaaggtt gccacagacg ttgatactgg ttaagacggt
    65641 tgagcagtcg catataaacc cggaacaata ttatttaact attcactatt acttccgtat
    65701 atatcaggtg atactcaatc accattaacc gtgtcacaga gtggagaaag aaatggctcg
    65761 tctggcagca tttgatatgg atggcacttt attgatgcct gaccatcatt taggtgagaa
    65821 aaccctctct actttggcgc gactgcgtga acgcgacatt accctcactt ttgccactgg
    65881 gcgtcatgcg ctggagatgc agcatattct cggggcgcta tcgctggatg catatttgat
    65941 taccggcaac ggaacgcgcg tgcattctct ggaaggtgaa cttttacatc gtgatgattt
    66001 acctgcggat gtcgcggagc tggtgctgta tcagcagtgg gatacccgag ccagcatgca
    66061 tatcttcaat gacgacggtt ggtttaccgg gaaagagatc cctgcgttgt tgcaggcatt
    66121 tgtctatagt ggttttcgtt atcagataat cgatgtcaaa aaaatgccac tcggcagcgt
    66181 caccaagatc tgcttctgtg gcgatcacga cgatcttaca cgcttgcaga tccagctata
    66241 cgaagcatta ggcgagcgtg cacatttgtg tttttccgcc acggattgcc tcgaagtgct
    66301 gccggtgggc tgcaataaag gcgctgcatt gacggtgctg acccaacatt taggtttatc
    66361 gttgcgcgat tgcatggcct ttggtgatgc gatgaacgat cgcgaaatgt taggcaacgt
    66421 cggtagcgga tttattatgg gcaatgcgat gccgcaattg cgcgcggagc tcccgcattt
    66481 accggtgatt ggacattgcc gaaatcaggc tgtctctcac tatttgacgc actggctgga
    66541 ctatccacat ctaccttatt cccccgaata acgagatccc ttccagcacc gggcaattgc
    66601 ccggtttttt ttgcgttgaa tttgtcattt tgtgccgtgg tgtttaaacc gcacagaata
    66661 aattgtcgcg atttcacctt taaaatagaa ttaaaagaga aaaaaattct ctgtggaagg
    66721 gctatgttag ataaaattga ccgtaagctg ctggccttac tacagcagga ttgtaccctc
    66781 tctttgcagg cactggctga agccgttaat ctgacaacca ccccgtgctg gaagcgtctg
    66841 aaacggctgg aggacgacgg tattcttatc ggcaaagtcg ccctgctgga tccggaaaaa
    66901 ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc aacatcacag cagcgaatgg
    66961 tattgccgct ttgtcacggt ggttaccgaa atgccagaag tgctggggtt ctggcgcatg
    67021 gccggtgaat acgattatct gatgcgcgtc caggttgccg acatgaaacg ctacgacgag
    67081 ttttataagc gtttggtaaa cagcgtgccg gggctgtcgg acgtcacttc cagcttcgcg
    67141 atggaacaga ttaaatacac cacttcttta cccatcgaat aaatatccag aatcaggtca
    67201 ggacacaacg cgtgcgatta tttgctcaat taagctggta tttccgtcgg gaatggcgtc
    67261 gctatctcgg ggctgtcgcc ttgcttgtca ttatcgcgat gctgcaactg gttccgccaa
    67321 aagtggttgg tattgttgtt gatggcgtga cagaacaaca ctttactacc gggcagatcc
    67381 tgatgtggat cgccaccatg gtgctgattg ccgttgtggt ttatctcctg cgttacgtct
    67441 ggcgtgtatt gctgtttggc gcgtcttatc aactggctgt tgaactgcgt gaagattatt
    67501 accgtcagct aagccggcag catcctgagt tttacctgcg tcatcgcacg ggtgacctca
    67561 tggctcgtgc gaccaatgac gtcgatcgcg tcgtgtttgc cgccggagaa ggggtgctga
    67621 cgctggtgga ttcactggta atgggctgcg ctgtgttgat tatgatgtct acgcaaatta
    67681 gctggcagtt gaccttattt gccctgttgc cgatgccagt gatggcgatc atgattaagc
    67741 gcaacggcga tgccttgcat gaacgcttta agctggcgca ggcggcgttt tccagtctta
    67801 atgaccgcac ccaggaaagc ctcaccagta tccgcatgat caaagctttt ggtctggaag
    67861 atcgccagtc ggcgttattt gccgaggatg ccgaagatac cggcaaaaaa aacatgcggg
    67921 tggcgcgtat tgatgctcgt ttcgacccga ccatctatat cgcgattggt atggcgaact
    67981 tgctggcgat tggcggcggt agttggatgg tggtgcaggg cagtttaacg ctgggccagc
    68041 tcaccagttt tatgatgtat ttaggtctga tgatttggcc aatgctggcg ctggcatgga
    68101 tgtttaacat agtggaacgt ggtagtgctg cgtacagccg cattcgcgcg atgctggcgg
    68161 aagcgccggt ggtgaatgat ggtagcgaac ccgtgccgga aggtcgtggc gaactggatg
    68221 taaatattcg ccagttcacg tatccgcaga ctgaccatcc tgcgctggaa aacgtcaatt
    68281 tcgccctgaa acccggtcag atgctgggta tttgcgggcc gactggttcc ggcaaaagta
    68341 ccctgttgtc gctcattcag cgtcatttcg acgtcagcga gggggatatc cgctttcatg
    68401 atattcctct gacgaagtta caactcgata gctggcgtag ccgcctggcg gtagttagcc
    68461 agacaccgtt ccttttttcc gacaccgtag cgaacaacat cgcgttgggt tgcccgaatg
    68521 ccacccagca agagattgag catgtcgcgc ggttagccag cgtgcatgac gatattttgc
    68581 gtttaccgca aggttacgat acagaagtgg gcgagcgcgg cgtgatgctt tccggtgggc
    68641 aaaaacagcg tatctccatt gctcgtgcgt tattagtcaa cgcggaaatc ctcatccttg
    68701 atgatgcgct ttcggcagta gacggacgca cagagcacca gatcctgcat aacctgcgtc
    68761 agtgggggca gggaagaacg gtaatcatca gcgcccatcg cctttctgca ctgacggaag
    68821 ccagtgaaat tattgtgatg cagcacggac atatcgctca gcgtggcaat catgatgagc
    68881 tggcgcaaca aagcggctgg tatcgcgata tgtatcgcta tcaacaactg gaggcggcgc
    68941 tcgacgacgc tccggaaatt cgcgaggagg ccatcgatgc gtagttttag ccaactgtgg
    69001 ccgactctca agcgtctgtt agcgtacggt tcgccgtggc gtaaaccgct ggggattgcg
    69061 gtcataatga tgtgggttgc ggcagcggca gaagtcagtg ggccgctgct tatcagctat
    69121 tttatcgaca atatggtagc gaaaaataac ctgccgttga aagtggttgc agggctggct
    69181 gcggcgtatg ttgggctgca actgtttgcc gccgggctac attacgcgca gtcgctgctg
    69241 tttaatcggg cggcagtagg cgtagtgcaa cagttgcgta ccgacgtgat ggatgctgcg
    69301 ttacgccagc cattaagcga gtttgatacc caacccgtcg ggcaggtgat ttcccgcgtc
    69361 actaatgaca ctgaagtgat ccgcgacctc tacgttaccg tagtggcaac tgtcctgcgt
    69421 agcgccgcac tggtgggcgc gatgctggtg gcgatgttca gcctcgactg gcgaatggcg
    69481 ctggtggcga taatgatttt cccggtggtg ctggtggtaa tggtgatata tcagcgttac
    69541 agcacgccga ttgtccgtcg tgtgcgcgct tatctggcag atatcaacga cggctttaac
    69601 gaaattatca atggcatgag cgttatccag cagttccgtc agcaggcgcg atttggcgaa
    69661 cgtatgggtg aggccagtcg ttcacactat atggcgagga tgcaaaccct gcgcctcgac
    69721 ggttttctgc tgcgtccgct gctgagtctg ttttcatcgc tcattctttg tggcttgttg
    69781 atgctgtttg gcttctccgc cagcggcacc attgaagtgg gcgtgttgta tgcgttcatc
    69841 agttatttgg ggcgacttaa cgaaccctta atcgaactga ccacgcagca ggcgatgctg
    69901 caacaggctg ttgttgctgg tgagcgcgtt tttgagctga tggacggacc gcgccagcaa
    69961 tatggcaatg atgatcgccc gttgcagagc ggcaccatcg acgtcgataa cgtgtcattt
    70021 gcttatcgcg atgacaatct ggtgctaaag aacattaatc tctctgtgcc ttcgcgcaac
    70081 tttgtggcgc tggtcgggca taccggcagt ggcaaaagca ccctcgccag tttattgatg
    70141 ggctattacc cgctaacgga aggtgagatt cgccttgatg gtcgtccatt aagtacgcta
    70201 agtcacagcg cgctgcgcca gggcgtggca atggtgcagc aagatccggt ggtgctggcg
    70261 gataccttcc tcgccaacgt gacgctggga cgtgatatct ctgaagaacg cgtctggcag
    70321 gcgctggaaa tcgtgcagct ggcggagctg gcgcgtagca tgagtgatgg tatttacacg
    70381 ccgcttggcg agcaggggaa taatctctca gtcgggcaaa agcaactgct ggcactggcg
    70441 cgcgtgctgg tggagacgcc gcaaatcctg atccttgatg aggcaaccgc cagcattgac
    70501 tccgggactg aacaggcgat tcaacatgct ctggcggcgg tgcgtgaaca tactacgctt
    70561 gtggtgattg ctcaccgctt atcgactatt gttgatgcca acaccattct ggtgcttcat
    70621 cgtgggcaag ccgtggagca gggcactcac cagcaactgc tggcggccca gggacgatac
    70681 tggcagatgt atcaactgca acttgcgggc gaagagctgg cagccagcgt gcgtgaagag
    70741 gaatcattga gcgcctgaat agcgcaatat ttcatcgttg gtgcaaaaat gtaacgcact
    70801 gtgcactgtc atagtgcgtt ttcattttca atcttcttaa cttcctgctc tctttctcgt
    70861 ttttcatttt tggcacaccg cttgcaatac cttcttcgtg tagcagaacc attaccgaat
    70921 tctgaccgga ggggatctat gaagctggtg accgtgatta tcaaaccatt caaacttgaa
    70981 gacgttcgtg aagcgttatc ttccattggt attcagggcc tgaccgtcac cgaagtgaaa
    71041 ggtttcgggc gtcagaaagg acatgccgag ctgtaccggg gggcggaata cagcgtcaat
    71101 ttcctgccaa aagtaaaaat tgatgtggcg attgctgatg accaactcga tgaagtgatc
    71161 gatatcgtca gtaaggcggc ttacaccgga aaaattggcg acggcaaaat cttcgtcgct
    71221 gaattgcaac gcgtcattcg tattcgtacc ggcgaagccg acgaagcggc gctgtaatct
    71281 ctggcacaca gcaacaggaa cgaaaaatga agatagcgac gataaaaact gggcttgctt
    71341 cactggcgat gctgccggga ctggtaatgg ctgcacctgc ggtggccgat aaagccgaca
    71401 atgcgtttat gatgatttgt actgcgctgg tgctgtttat gactattccg gggattgccc
    71461 tgttttacgg tgggttgatt cgcggcaaaa acgtgctgtc gatgctgacg caggtgacgg
    71521 tgacgtttgc actggtctgt attctctggg tggtttacgg ttactcgctg gcgtttggtg
    71581 agggaaacaa cttcttcggc aacattaacg ggttgatgct gaaaaacatc gaactgacgg
    71641 cggtgatggg cagcatttat cagtatatcc acgtagcgtt tcagggatcg tttgcctgca
    71701 ttaccgtagg cttgatagtt ggagcactgg cggaacgaat ccgcttctca gctgtgttga
    71761 ttttcgtggt ggtatggctg acgctctcct acattccgat tgcgcatatg gtctggggcg
    71821 gcggtttgct ggcttctcac ggtgcactgg atttcgcggg tggcacggtg gtgcacatta
    71881 acgccgcaat cgccggtctg gtgggcgcgt atctgatagg caaacgcgtg ggcttcggca
    71941 aagaggcgtt taaaccgcac aacctgccga tggtcttcac cgggactgcc attctctata
    72001 tcggttggtt tggctttaac gccgggtcag cgggcacggc gaatgaaatc gcagcactgg
    72061 catttgtgaa tactgtggtc gcaacggcgg cggcaattct tggctggatc ttcggtgaat
    72121 gggcgctgcg tggtaagcct tcactgctgg gagcgtgttc aggcgcgatt gccggtctgg
    72181 tcggcgtgac gccagcgtgt ggctacattg gtgttggcgg cgcgttgatt atcggcgtgg
    72241 tagctggtct ggcgggcttg tggggcgtta ccatgctcaa acgcttgttg cgggtggatg
    72301 atccctgcga tgtcttcggt gtgcacggcg tttgtggcat tgtcggctgt atcatgaccg
    72361 ggatttttgc tgccagctcg ctgggcggcg tgggcttcgc tgaaggtgtg acgatgggcc
    72421 atcagttgct ggtacagctg gaaagcatcg ccattacgat cgtctggtcc ggtgtggtgg
    72481 catttattgg ctacaaactg gcggatctga cggttggtct gcgtgtgccg gaagaacaag
    72541 agcgagaagg gctggacgtc aacagccacg gcgagaatgc ctataacgcg taacaagcac
    72601 tgcaaaaaac agccggacgg ttttcacctc cggctatttt tttaattatg attacgcatt
    72661 accccttcct gaacggtcga ggcaaccagc acgccgtcct gagtataaaa ctcaccgcgc
    72721 acaaagccgc gtgcgctgga cgccgaggtg ctctccacgc tatacagcag ccattcattc
    72781 aaattaaacg ggcgatggaa ccacatggaa tggtcgatgg tcgctatctg aattcccggt
    72841 tcgagaaaac cgatgccgtg cggctgtaga gctaccggca ggaagttaag atcagaagcg
    72901 taaccgagca gatactgatg aacgcgcaga tcatccggca cgctaccatt tgcgcggatc
    72961 cacacctgac gatgcggttc tgcgacgtga cctttcaatg ggttatgaaa ttccaccgga
    73021 cggacttcca gcggacgatc gcagatgaat ttatctttca gcactggcgg cagcaggtgc
    73081 gccagcgatt gggcgatttg tgtttccgaa ggcaggccat caggcgctgg cgcggacggc
    73141 attgtttttt gatgttcgaa acccgcttct ggtgcctgga aagaggccgt catataaaaa
    73201 atcggtttgc cgttttgaat agcagcaacc cggcgggcgc tgaagctgtt accgtcacgc
    73261 agcgtttcga catcataaat aatcggcttc ttactatcgc ctgggcgaag aaagtagctg
    73321 tgaaacgaat gtaccagccg ctcttcaggg acggtctctt ttgcagcata caaggcctga
    73381 cccacgacct ggccgccaaa cacctggcgt aaacctaaat cttcactctg gccgcgaaag
    73441 agtccttcct caattttttc cagatttaac aatgtcagta aattttttag cgcctgactc
    73501 atataactct ccagtaacaa agctgccgca gcaagccaaa gtgagttgag tataacgcaa
    73561 atttgccact ggtccgatgg gtgcaatggt ctgaattacg ggctaattac aggcagaaat
    73621 gcgtgatgtg tgccacactt gttgacgtta ctattttgtt aaccactctt ccggcgagga
    73681 aagttagccc gctggtgcat tgataataag gagaagtgaa tgaaactcgt gcacatggcc
    73741 agtggtttag cggttgcgat tgcgttggcg gcttgcgcag ataaaagcgc ggatattcag
    73801 acgccagcac cggctgcaaa tacgtctatt tcagcaacac aacctgctat ccagcaaccg
    73861 aatgtctccg gtaccgtctg gatccgtcag aaagtcgcac tgccgcctga tgctgtgctg
    73921 accgtgacac tttctgacgc gtcgttagcc gatgcgccgt caaaagtgct ggcgcagaaa
    73981 gcggtgcgta ccgaaggtaa acagtcacca ttcagctttg ttctgccatt taacccggca
    74041 gatgttcagc cgaacgcgcg tattctattg agtgcggcga ttaccgtgaa tgacaaactg
    74101 gtatttatta ccgataccgt tcagccggtg atcaatcagg gcggaactaa agccgacctg
    74161 acattggtgc cggtgcagca aaccgccgtg ccggttcagg ccagcggtgg cgcaacggct
    74221 accgtacctt cgacttcacc aactcaggtg aatccgtctt cggcagttcc agctcctacg
    74281 caatattaag cgcagttaac cctctccgcc cggagagggt tagtagttcc agcgataacg
    74341 ctgcaagtcg atttgcccgc ttcccgatac catcacacct tctgccagta atgcctgtcg
    74401 ttgacgctgt aaatccggtc cggttagcga aattgtgccg tggcgattaa ccacccggtg
    74461 ccagggcaag gtgctgcctt cggggagacg ctttaacaca ccgcctacct ggcgcgcggc
    74521 gcgcggcgat cccgccagtt tcgccacatc accgtaagtg gtgacatagc cttcgggaat
    74581 agcggcgacg atttgccaga cgcgttgggg aaatgaatct tctttttcca tcttttcttc
    74641 ctgaggtaat ttttcagcat aatctggaaa aacgcccgag tgaagccgca ttgcgcaaga
    74701 aaccagcatc tggcacgcaa tgggttgcaa ttagccgggg cagcagtgat aatgcgcctg
    74761 cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt tctcccgcaa cgctactctg
    74821 tttaccaggt caggtccgga aggaagcagc caaggcagat gacgcgtgtg ccgggatgta
    74881 gctggcaggg cccccaccca tttctgcctc cccccgtttc gtcaaaaaat cccaacatgg
    74941 ctaaacttta accacaactg acgtcgcaag aattgtctgg ctgcgcagta cgcttcggag
    75001 gtatgtctga tgaagtatgt tgatggtttt gttgttgccg ttcctgccga taaaaaggat
    75061 gcctatcggg aaatggccgc taaggccgcg ccattattta aagagttcgg tgcgcttcgt
    75121 attgtcgaat gctgggccag cgatgtaccg gatggtaaag tgaccgattt tcgtatggcg
    75181 gtgaaagcgg aagagaatga agaggttgtc tttagctgga ttgaataccc ttcaaaagag
    75241 gtccgcgacg ctgctaatca aaagatgatg tcggacccac ggatgaaaga gttcggcgag
    75301 tccatgccgt ttgacggcaa gcgaatgatc tatggtggat tcgaatcaat catcgacgag
    75361 taggtagcgt gacgggctgt gcggcgtgcc gtcagcccta aaattcacaa atgttgctcg
    75421 gcccagcgta agaaatcctc tttcggtaat gccttgctgt agagccagcc ctgaccgtag
    75481 tgcacgccat gctggcgtaa ccactcttct tgtttactgg tttcgattcc ctccgctacc
    75541 attttcagtt tcagtgtttt tgccatttcg atgatatgcg gcgtgacatt tttatattcc
    75601 agcgcatcaa cgaaagattt atcgatcttc agaatgtcga catccagatc ctgtaaataa
    75661 cttaaacttg aataccccgt accaaaatca tcgagataaa tttcatggcc cgcctcccgg
    75721 tagcgagaaa ttatcggggc gctggttttc ggatcggcaa actcgcgttc agtgagttca
    75781 agcgcgatct gtctgggatt aacctgatag tgattgatca tttcacgcag caattgcggg
    75841 attttttccg aggtgagcac ggtggattca agattgatcg aaatatgctg ctgcggatgc
    75901 tgacgcagcc agtcgcccat atcttcgaag acgcttctta tgatcagtag cgtcaacggc
    75961 tcagaaaggc ccgtttgctg tgccagcgga ataaaactat ctggtgacaa ccaactaccg
    76021 tctgtctgcg gccagcgcgc cagtgcctca gcaccgacaa ttttgccatt ggctaaggag
    76081 acaatcggct gatagtgcac gcaaatatca cgattttcga tagcatcctg cagccgatga
    76141 tgcggtgact ggatacggcg caaaatacgc agcacaaaca tcgctgccag caggccaatc
    76201 accaacccga gcggtaacca aataaagact tgccgatgcc agcctttctg caacattttc
    76261 gttgaagacc aggtgatgat cgaaatattc atttccggga agggcaggat atcgtaaatg
    76321 attccattat tttcgatatg ctcaccgggt gttttttgta gtctggtaat aattccctga
    76381 gcaatttcat cgctgctggt tatcacaacg ttatgggcat tgccaataat ggcggcatca
    76441 atttgccatg agctataggg aatgacatca ataaaggaag cggggtcgat catgacgaca
    76501 taatgtgccg ttcccatggc aaccatgtaa cggataatgc ctaaatcgtt atgcgatgtt
    76561 aaccagacac gataaccatc tttcgaaatt ttacctggct cggggaaggt atcgggcggg
    76621 ctttcatgct ccagagacga acactgggga acgttgttat cgatataaac cacttcctga
    76681 atatagcgat aactgtaaga tacccgacgc atttccatga gatgggcttc gctacaggca
    76741 gcgccttgcc atctttccag ctcttgcagc gcatctttcc cttgtgtcgc caccttattg
    76801 gcccgaatag cgacgcggga ggaatacgta ttcagctctt caataaatga tgtttctacc
    76861 tgctgatggg ccagccagat gcttaagccg acaggcagca atactgaaag aatcagtact
    76921 cccgaaataa ggccgaccag atgtcgtgtt ctcaccagca tatccttatt aacacagtgt
    76981 agggaaagta tatctgattg tggaaaaagg ctggcaggag ataaggaggt agagccggga
    77041 tttcatgaag ggatgggtgg aaacgaacgt tgtcatttga ccaggcgcag agctttacgc
    77101 ctggtatgta accctaactt aagatgttga ttcggcgcga gcgagggtaa aaatatcgta
    77161 aaaagatagt tcacctttac ggacgatcat tttttgcagt tgttctttat gaacatccgc
    77221 gacacgcgga gagttcagga tggcttcaac tagcgcggca ggtacaaagc gcgtgttgta
    77281 ccattcgccg ttatagcaaa cgcggaaatc cagcacgtcg atatcttcat agcggtagcg
    77341 tgggtagaca tcgaaaaaga agaaaccatt aagcaggata aataatacaa ccagtagcca
    77401 gacagagcct gaaagcgttt cggactgcaa catcactgcc agggtggcaa aaaaagcaac
    77461 gtacataccg ataaacaacc ccggatgttt acggataaaa ctgatactaa agcggggttt
    77521 gttgtcgcgt ttttcgcggg tatttagaga ctcaattgtt tcggtcagca ggcgttgtat
    77581 ttcggtcatc gtttgccttc gtggtatctg caactttcca gggaacaccc tattttaacg
    77641 tgggaatagt agagggaaag taacagattt accacaattt tgcataacag ttgcgaaaaa
    77701 ccgattacaa ttttttaatt attctggctg gattaccgcc cacgacaacg ttgtccggga
    77761 catcttttgt gacaactgca cctgaggcta ccacgacgtt atcaccaatg gtcacaccag
    77821 ggttaatgac cgcacgtccg ccaatccaga cgttattacc gatggtgacg ggtttcccca
    77881 gttcagcacc gctattacgt gctacagggt cgatgggatg tgttgccgtg tagatatgaa
    77941 cgcctggtgc caacatacag ttatcaccaa tgcgaatagg gcagacatca agcatcacgc
    78001 aatcgaagtt ggcgaaaaaa ttattgccga gaaaaatgtt ataaccatag tcacagcgaa
    78061 acgttggctc aatataagcc tctgtcacct gaccgaataa atcagcaaga atttgctggc
    78121 gtaatgtgtg ctcttccgcc agggaatgat tgtatcggtg aataagccga cgagcgcgca
    78181 ggcgatcgcg agataacgcc tcatctgccg agtgatacaa ctcaccagca atcatctttt
    78241 ctttttctgt gctcattact gacctccggt tgctgaaaat gcggcaacac agtaagacga
    78301 aaagtcagtg aagagaacgt tcccattaca tctgtgtgat gaagatcaca tagggtatgc
    78361 cttcatggcg aaagaattat aaaactctta ataaacagcc ggttatagct ccgaaagcga
    78421 gttatttatt agcgaataaa tttccatact gaggaaggga tcttgtcgta cagtttattc
    78481 atggtcaatt cggcgaggcg gtgatctgcg gctgagtaaa ataccgccag ctcattatct
    78541 gataattcgt atttattttt ctcgataaca cgctccagcg tgtcaattgt ctggcaacga
    78601 cgtaaacgca ttaaataatc ggtttttgtt aaaggttttt cggacatact tctacccatg
    78661 gttgtaataa ttctaacaag ataaactcgc aggattctct ttcgtcgcat tgacaaaaca
    78721 acggaatagt cgattacctg acttccgcca tttctgaaga tcctgcatat taataccata
    78781 actactgaac aacataaagg tgtcatccag atattcgtcg atctgctcaa tgagcttatt
    78841 gtcttcatta tacttaattt tgtaattaag tgcgaaggtc gcaatatgct caatcagttc
    78901 attcaactgg aggttgatcg ccgaggttgg gtcgttaacc cagccatgat tgctttcttc
    78961 aaggtttgca agacagtcat gatacagggt ttcacagaga aacttaagct gcgcgatatc
    79021 atgtcttttg ggtgagtatt catccataac gcgtcccctt cttagcggtt gaactaacgg
    79081 acacctttcg ggatggaaaa aacttactga cctggacttg ccctctcgtt tgttagctta
    79141 actataagcc actctttgca ggttttcatc gcattattac gaaaaattac aattaggaag
    79201 gaagcgggac aggactcact ttttcatact agcactaacc cgcagcaggt aaaagcagtt
    79261 tgctcgaact gttcataaca tttcaggatt atcgccagat gaaattggta acactaat
//
LOCUS       DAZGTJ010000012        76505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000012 DAZGTJ010000000
VERSION     DAZGTJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.012"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            113..1042
                     /gene="pfkB"
                     /locus_tag="ACHK8E_001072"
     CDS             113..1042
                     /gene="pfkB"
                     /locus_tag="ACHK8E_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP0343372.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1143..1433
                     /gene="ghoS"
                     /locus_tag="ACHK8E_001073"
     CDS             1143..1433
                     /gene="ghoS"
                     /locus_tag="ACHK8E_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0343373.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            1539..2399
                     /gene="yniA"
                     /locus_tag="ACHK8E_001074"
     CDS             1539..2399
                     /gene="yniA"
                     /locus_tag="ACHK8E_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP0343374.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2440..2976)
                     /gene="yniB"
                     /locus_tag="ACHK8E_001075"
     CDS             complement(2440..2976)
                     /gene="yniB"
                     /locus_tag="ACHK8E_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP0343375.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3123..3791
                     /gene="hxpB"
                     /locus_tag="ACHK8E_001076"
     CDS             3123..3791
                     /gene="hxpB"
                     /locus_tag="ACHK8E_001076"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP0343376.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3955..4545
                     /gene="ydjM"
                     /locus_tag="ACHK8E_001077"
     CDS             3955..4545
                     /gene="ydjM"
                     /locus_tag="ACHK8E_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0343377.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4678..6069
                     /gene="tcyP"
                     /locus_tag="ACHK8E_001078"
     CDS             4678..6069
                     /gene="tcyP"
                     /locus_tag="ACHK8E_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP0343378.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6116..6379)
                     /gene="cedA"
                     /locus_tag="ACHK8E_001079"
     CDS             complement(6116..6379)
                     /gene="cedA"
                     /locus_tag="ACHK8E_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP0343379.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6562..8823
                     /gene="katE"
                     /locus_tag="ACHK8E_001080"
     CDS             6562..8823
                     /gene="katE"
                     /locus_tag="ACHK8E_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP0343380.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(9081..9839)
                     /gene="chbG"
                     /locus_tag="ACHK8E_001081"
     CDS             complement(9081..9839)
                     /gene="chbG"
                     /locus_tag="ACHK8E_001081"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP0343381.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(9852..11204)
                     /gene="celF"
                     /locus_tag="ACHK8E_001082"
                     /gene_synonym="chbF"
     CDS             complement(9852..11204)
                     /gene="celF"
                     /locus_tag="ACHK8E_001082"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0343382.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11308..12150)
                     /gene="chbR"
                     /locus_tag="ACHK8E_001083"
     CDS             complement(11308..12150)
                     /gene="chbR"
                     /locus_tag="ACHK8E_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP0343383.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(12158..12508)
                     /gene="chbA"
                     /locus_tag="ACHK8E_001084"
     CDS             complement(12158..12508)
                     /gene="chbA"
                     /locus_tag="ACHK8E_001084"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP0343384.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12559..13917)
                     /gene="chbC"
                     /locus_tag="ACHK8E_001085"
     CDS             complement(12559..13917)
                     /gene="chbC"
                     /locus_tag="ACHK8E_001085"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP0343385.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(14002..14322)
                     /gene="chbB"
                     /locus_tag="ACHK8E_001086"
     CDS             complement(14002..14322)
                     /gene="chbB"
                     /locus_tag="ACHK8E_001086"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP0343386.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14621..14959)
                     /gene="osmE"
                     /locus_tag="ACHK8E_001087"
     CDS             complement(14621..14959)
                     /gene="osmE"
                     /locus_tag="ACHK8E_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP0343387.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            15161..15988
                     /gene="nadE"
                     /locus_tag="ACHK8E_001088"
     CDS             15161..15988
                     /gene="nadE"
                     /locus_tag="ACHK8E_001088"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP0343388.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            16218..17105
                     /gene="cho"
                     /locus_tag="ACHK8E_001089"
     CDS             16218..17105
                     /gene="cho"
                     /locus_tag="ACHK8E_001089"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP0343389.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(17065..17640)
                     /gene="ves"
                     /locus_tag="ACHK8E_001090"
     CDS             complement(17065..17640)
                     /gene="ves"
                     /locus_tag="ACHK8E_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP0343390.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17843..18328)
                     /gene="spy"
                     /locus_tag="ACHK8E_001091"
     CDS             complement(17843..18328)
                     /gene="spy"
                     /locus_tag="ACHK8E_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP0343391.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(18658..19626)
                     /gene="astE"
                     /locus_tag="ACHK8E_001092"
     CDS             complement(18658..19626)
                     /gene="astE"
                     /locus_tag="ACHK8E_001092"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP0343392.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(19619..20962)
                     /gene="astB"
                     /locus_tag="ACHK8E_001093"
     CDS             complement(19619..20962)
                     /gene="astB"
                     /locus_tag="ACHK8E_001093"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP0343393.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(20959..22437)
                     /gene="astD"
                     /locus_tag="ACHK8E_001094"
     CDS             complement(20959..22437)
                     /gene="astD"
                     /locus_tag="ACHK8E_001094"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP0343394.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(22434..23468)
                     /gene="astA"
                     /locus_tag="ACHK8E_001095"
     CDS             complement(22434..23468)
                     /gene="astA"
                     /locus_tag="ACHK8E_001095"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP0343395.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(23465..24685)
                     /gene="astC"
                     /locus_tag="ACHK8E_001096"
     CDS             complement(23465..24685)
                     /gene="astC"
                     /locus_tag="ACHK8E_001096"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP0343396.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            25131..25937
                     /gene="xthA"
                     /locus_tag="ACHK8E_001097"
     CDS             25131..25937
                     /gene="xthA"
                     /locus_tag="ACHK8E_001097"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP0343397.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            26104..26814
                     /gene="ydjX"
                     /locus_tag="ACHK8E_001098"
     CDS             26104..26814
                     /gene="ydjX"
                     /locus_tag="ACHK8E_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0343398.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            26819..27496
                     /gene="ydjY"
                     /locus_tag="ACHK8E_001099"
     CDS             26819..27496
                     /gene="ydjY"
                     /locus_tag="ACHK8E_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP0343399.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            27510..28217
                     /gene="ydjZ"
                     /locus_tag="ACHK8E_001100"
     CDS             27510..28217
                     /gene="ydjZ"
                     /locus_tag="ACHK8E_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0343400.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            28217..28765
                     /gene="ynjA"
                     /locus_tag="ACHK8E_001101"
     CDS             28217..28765
                     /gene="ynjA"
                     /locus_tag="ACHK8E_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0343401.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            28775..29941
                     /gene="ynjB"
                     /locus_tag="ACHK8E_001102"
     CDS             28775..29941
                     /gene="ynjB"
                     /locus_tag="ACHK8E_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0343402.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            29914..31449
                     /gene="ynjC"
                     /locus_tag="ACHK8E_001103"
     CDS             29914..31449
                     /gene="ynjC"
                     /locus_tag="ACHK8E_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP0343403.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            31449..32102
                     /gene="ynjD"
                     /locus_tag="ACHK8E_001104"
     CDS             31449..32102
                     /gene="ynjD"
                     /locus_tag="ACHK8E_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0343404.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            32169..33476
                     /gene="ynjE"
                     /locus_tag="ACHK8E_001105"
     CDS             32169..33476
                     /gene="ynjE"
                     /locus_tag="ACHK8E_001105"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP0343405.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(33485..34105)
                     /gene="ynjF"
                     /locus_tag="ACHK8E_001106"
     CDS             complement(33485..34105)
                     /gene="ynjF"
                     /locus_tag="ACHK8E_001106"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0343406.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            34192..34599
                     /gene="nudG"
                     /locus_tag="ACHK8E_001107"
     CDS             34192..34599
                     /gene="nudG"
                     /locus_tag="ACHK8E_001107"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP0343407.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(34565..34837)
                     /gene="ynjH"
                     /locus_tag="ACHK8E_001108"
     CDS             complement(34565..34837)
                     /gene="ynjH"
                     /locus_tag="ACHK8E_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFP0343408.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            35073..36416
                     /gene="gdhA"
                     /locus_tag="ACHK8E_001109"
     CDS             35073..36416
                     /gene="gdhA"
                     /locus_tag="ACHK8E_001109"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP0343409.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(36493..38448)
                     /gene="topB"
                     /locus_tag="ACHK8E_001110"
     CDS             complement(36493..38448)
                     /gene="topB"
                     /locus_tag="ACHK8E_001110"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0343410.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(38453..39496)
                     /gene="selD"
                     /locus_tag="ACHK8E_001111"
     CDS             complement(38453..39496)
                     /gene="selD"
                     /locus_tag="ACHK8E_001111"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP0343411.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(39613..40164)
                     /gene="ydjA"
                     /locus_tag="ACHK8E_001112"
     CDS             complement(39613..40164)
                     /gene="ydjA"
                     /locus_tag="ACHK8E_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP0343412.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            40325..42181
                     /gene="sppA"
                     /locus_tag="ACHK8E_001113"
     CDS             40325..42181
                     /gene="sppA"
                     /locus_tag="ACHK8E_001113"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP0343413.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            42346..43362
                     /gene="ansA"
                     /locus_tag="ACHK8E_001114"
     CDS             42346..43362
                     /gene="ansA"
                     /locus_tag="ACHK8E_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP0343414.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            43373..44014
                     /gene="pncA"
                     /locus_tag="ACHK8E_001115"
     CDS             43373..44014
                     /gene="pncA"
                     /locus_tag="ACHK8E_001115"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP0343415.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(44175..44447)
                     /gene="yeaC"
                     /locus_tag="ACHK8E_001116"
     CDS             complement(44175..44447)
                     /gene="yeaC"
                     /locus_tag="ACHK8E_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP0343416.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(44489..44902)
                     /gene="msrB"
                     /locus_tag="ACHK8E_001117"
     CDS             complement(44489..44902)
                     /gene="msrB"
                     /locus_tag="ACHK8E_001117"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP0343417.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            45244..46239
                     /gene="gapA"
                     /locus_tag="ACHK8E_001118"
     CDS             45244..46239
                     /gene="gapA"
                     /locus_tag="ACHK8E_001118"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0343418.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            46323..47225
                     /gene="yeaD"
                     /locus_tag="ACHK8E_001119"
     CDS             46323..47225
                     /gene="yeaD"
                     /locus_tag="ACHK8E_001119"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP0343419.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(47276..48130)
                     /gene="yeaE"
                     /locus_tag="ACHK8E_001120"
     CDS             complement(47276..48130)
                     /gene="yeaE"
                     /locus_tag="ACHK8E_001120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP0343420.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(48220..48966)
                     /gene="mipA"
                     /locus_tag="ACHK8E_001121"
     CDS             complement(48220..48966)
                     /gene="mipA"
                     /locus_tag="ACHK8E_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP0343421.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            49402..51336
                     /gene="yeaG"
                     /locus_tag="ACHK8E_001122"
     CDS             49402..51336
                     /gene="yeaG"
                     /locus_tag="ACHK8E_001122"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP0343422.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            51449..52732
                     /gene="yeaH"
                     /locus_tag="ACHK8E_001123"
     CDS             51449..52732
                     /gene="yeaH"
                     /locus_tag="ACHK8E_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP0343423.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYVAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            52879..54354
                     /gene="cdgI"
                     /locus_tag="ACHK8E_001124"
     CDS             52879..54354
                     /gene="cdgI"
                     /locus_tag="ACHK8E_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0343424.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADQALREAKSEGGNKVIIHHI"
     gene            54535..56025
                     /gene="dgcJ"
                     /locus_tag="ACHK8E_001125"
     CDS             54535..56025
                     /gene="dgcJ"
                     /locus_tag="ACHK8E_001125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP0343425.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            56068..56571
                     /gene="yeaK"
                     /locus_tag="ACHK8E_001126"
     CDS             56068..56571
                     /gene="yeaK"
                     /locus_tag="ACHK8E_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP0343426.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(56572..56676)
                     /gene="yoaI"
                     /locus_tag="ACHK8E_001127"
     CDS             complement(56572..56676)
                     /gene="yoaI"
                     /locus_tag="ACHK8E_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP0343427.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            56846..57292
                     /gene="yeaL"
                     /locus_tag="ACHK8E_001128"
     CDS             56846..57292
                     /gene="yeaL"
                     /locus_tag="ACHK8E_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP0343428.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(57249..58070)
                     /gene="nimR"
                     /locus_tag="ACHK8E_001129"
     CDS             complement(57249..58070)
                     /gene="nimR"
                     /locus_tag="ACHK8E_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP0343429.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            58167..59348
                     /gene="nimT"
                     /locus_tag="ACHK8E_001130"
     CDS             58167..59348
                     /gene="nimT"
                     /locus_tag="ACHK8E_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP0343430.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            59403..59750
                     /gene="yeaO"
                     /locus_tag="ACHK8E_001131"
     CDS             59403..59750
                     /gene="yeaO"
                     /locus_tag="ACHK8E_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP0343431.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(59772..60026)
                     /gene="yoaF"
                     /locus_tag="ACHK8E_001132"
     CDS             complement(59772..60026)
                     /gene="yoaF"
                     /locus_tag="ACHK8E_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP0343432.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            60209..61234
                     /gene="dgcP"
                     /locus_tag="ACHK8E_001133"
     CDS             60209..61234
                     /gene="dgcP"
                     /locus_tag="ACHK8E_001133"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP0343433.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            61267..61365
                     /gene="yoaK"
                     /locus_tag="ACHK8E_001134"
     CDS             61267..61365
                     /gene="yoaK"
                     /locus_tag="ACHK8E_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP0343434.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            61368..61442
                     /gene="yoaJ"
                     /locus_tag="ACHK8E_001135"
     CDS             61368..61442
                     /gene="yoaJ"
                     /locus_tag="ACHK8E_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP0343435.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(61501..61749)
                     /gene="yeaQ"
                     /locus_tag="ACHK8E_001136"
     CDS             complement(61501..61749)
                     /gene="yeaQ"
                     /locus_tag="ACHK8E_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0343436.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(61897..62079)
                     /gene="yoaG"
                     /locus_tag="ACHK8E_001137"
     CDS             complement(61897..62079)
                     /gene="yoaG"
                     /locus_tag="ACHK8E_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP0343437.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(62083..62442)
                     /gene="yeaR"
                     /locus_tag="ACHK8E_001138"
     CDS             complement(62083..62442)
                     /gene="yeaR"
                     /locus_tag="ACHK8E_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP0343438.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(62615..63253)
                     /gene="leuE"
                     /locus_tag="ACHK8E_001139"
     CDS             complement(62615..63253)
                     /gene="leuE"
                     /locus_tag="ACHK8E_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP0343439.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(63380..64303)
                     /gene="dmlR"
                     /locus_tag="ACHK8E_001140"
     CDS             complement(63380..64303)
                     /gene="dmlR"
                     /locus_tag="ACHK8E_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP0343440.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            64406..65491
                     /gene="dmlA"
                     /locus_tag="ACHK8E_001141"
     CDS             64406..65491
                     /gene="dmlA"
                     /locus_tag="ACHK8E_001141"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP0343441.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            65742..67352
                     /gene="yeaV"
                     /locus_tag="ACHK8E_001142"
     CDS             65742..67352
                     /gene="yeaV"
                     /locus_tag="ACHK8E_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP0343442.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            67384..68508
                     /gene="yeaW"
                     /locus_tag="ACHK8E_001143"
     CDS             67384..68508
                     /gene="yeaW"
                     /locus_tag="ACHK8E_001143"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP0343443.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            68564..69529
                     /gene="yeaX"
                     /locus_tag="ACHK8E_001144"
     CDS             68564..69529
                     /gene="yeaX"
                     /locus_tag="ACHK8E_001144"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP0343444.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(69576..70691)
                     /gene="rnd"
                     /locus_tag="ACHK8E_001145"
     CDS             complement(69576..70691)
                     /gene="rnd"
                     /locus_tag="ACHK8E_001145"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP0343445.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(70773..72458)
                     /gene="fadD"
                     /locus_tag="ACHK8E_001146"
     CDS             complement(70773..72458)
                     /gene="fadD"
                     /locus_tag="ACHK8E_001146"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP0343446.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(72663..73244)
                     /gene="yeaY"
                     /locus_tag="ACHK8E_001147"
     CDS             complement(72663..73244)
                     /gene="yeaY"
                     /locus_tag="ACHK8E_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP0343447.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(73283..73978)
                     /gene="tsaB"
                     /locus_tag="ACHK8E_001148"
     CDS             complement(73283..73978)
                     /gene="tsaB"
                     /locus_tag="ACHK8E_001148"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP0343448.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(74036..75946)
                     /gene="yoaA"
                     /locus_tag="ACHK8E_001149"
     CDS             complement(74036..75946)
                     /gene="yoaA"
                     /locus_tag="ACHK8E_001149"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP0343449.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            76078..76422
                     /gene="yoaB"
                     /locus_tag="ACHK8E_001150"
     CDS             76078..76422
                     /gene="yoaB"
                     /locus_tag="ACHK8E_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0343450.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        18808 a        19769 c        18812 g        19116 t
ORIGIN      
        1 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
       61 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
      121 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
      181 tcccgaagga aagctgcgct gtaccgcacc ggtattcgaa cccggaggcg gcggcatcaa
      241 cgtcgcccgc gccattgccc atcttggagg cagtgctaca gcgatcttcc cggtgggagg
      301 tgcgaccggc gaacatctgg tttcactgtt ggcagatgaa aatgtccccg tcgctactgt
      361 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgagca
      421 gtatcgtttt gtcatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
      481 gcaagttctg gaaattgaat ccggtgctat cctggtcata agcggaagcc tgccgccagg
      541 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
      601 tatcgtcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
      661 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
      721 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gagttgtcgt
      781 ttccctgggt ccacaagggg cgctgggcgt tgatagtgaa aactgcattc aggttgtgcc
      841 accaccggtg aaaagccaga gcaccgttgg cgctggtgac agcatggtcg gcgcgatgac
      901 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
      961 gagtgcagcc acactcaatc agggaacacg cctgtgctcc catgacgata cacaaaaaat
     1021 ttacgcttac ctttcccgct aactgaaaca ttcccccagc attgggggaa tcaccaccaa
     1081 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     1141 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcac gaagatacat
     1201 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     1261 cggatgatga tggaaatatc catgagctgg ggactaatac ttttggactt atcagtaccc
     1321 aaagtgaaga agaaattaga gaacttgttt cggggcttac ccaaagtgca accggtaaag
     1381 atcctgaaat agccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     1441 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     1501 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     1561 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     1621 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     1681 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     1741 gtaaaaccgt caccgtgcct aaggtttggg cagttggcgc tgaccgtgac tacagttttc
     1801 tggtgatgga ttatctccca cctcgcccgc tggatgcgca tagcgcattt attcttggtc
     1861 agcaaattgc gcgtttacat caatggagcg accaaccgca atttggcctc gatttcgata
     1921 acgcgctctc cacaactccc cagcccaaca cctggcaacg tcgctggtca actttttttg
     1981 ctgaacaacg gattggctgg cagctggaac tggcggcaga aaaagggatc gctttcggca
     2041 atatcgatgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     2101 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     2161 ttttcgaccc ggcctgctac tggggtgacc gagagtgcga cctggcgatg ttaccgctgc
     2221 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     2281 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaaggttat
     2341 ttggcggtca gcatttggtt attgctcagc agtcactgga tagattatta gcagcataat
     2401 atggggtgag gataatggcc gctccgtgcg gccttttgat taaataaacc ccagcagaga
     2461 aaagaacaca taacctgcag caatacaaat aaccggcagg atatacagcg agaaaaactg
     2521 caggaaaata gtatggtgcg gcacaacaat acgtgactca atttgttcac gcgttagccc
     2581 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     2641 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggaa
     2701 gaaaatcacc cagaacataa cgttcacgcc accattaaaa ttgggcgtcg gggagttata
     2761 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagtaaa
     2821 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttaag
     2881 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     2941 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     3001 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     3061 aggcgcttaa tcataagcac ggcttaatac cttacatata atgctctaaa ggagaggttg
     3121 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     3181 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtggata
     3241 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     3301 tttggtacgc ccggcaacca tggaatgggc caagccgtca ggaagtagta gaacaagtta
     3361 ttgctcgtgc catttcactg gttgaagaga cgcgtccatt attaccaggt gtgcgtgaag
     3421 ccgttgcgtt atgcaaagaa caaggtttat tggtggggct ggcctccgcg tcaccgctgc
     3481 atatgctgga aaaagtattg accatgtttg atttacgcga cagtttcgat gccctcgcct
     3541 cggctgaaaa attgccttac agcaagccgc atccgcaagt atatatcgac tgcgcagcaa
     3601 aactgggggt tgaccctctg acctgcgtgg cactggaaga ttcggtaaat ggcatgatcg
     3661 cctctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     3721 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     3781 ttctcggtta atgaccaggg ggcagtgatc acactgcccc tggttcttta tctgaatttg
     3841 cattcaactg acggattaat cgccaattta agagaaagag ttacaccgtc accacttccg
     3901 tgcactgtat aaaaacccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     3961 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     4021 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     4081 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa gtggatatca
     4141 aaaccaatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     4201 gcgctgctgg caacctttta ccttaaggtt cctgaaagct ggttcattcc ggctgatgcg
     4261 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     4321 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     4381 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     4441 ccccattcat tacccgaaaa cagcgctgtc cgttggtcat cgcaaatgat caataccttg
     4501 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     4561 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     4621 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     4681 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     4741 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     4801 gtttttggcc tcgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     4861 gtacagtggt ttaacattgt tggtaacggc tatgttcaac tcctgcaaat gatcgttatg
     4921 ccgttagtct ttgcctctat tctgagcgcg gttgcccgtc tgcataacgc atcacagtta
     4981 ggcaaaatca gttttctgac catcggaacg cttttgttta ccacactgat tgcggcgctg
     5041 gtcggtgtgc tggtcaccaa cctgtttggt ttgacggctg aaggtctggt tcagggtggt
     5101 gcagaaactg cacgtttgaa cgccatcgaa agtaactatg ttggtaaagt ctctgacctg
     5161 agcgttccgc agctggtctt gtcctttatc ccgaaaaacc cgtttgccga tttgaccgga
     5221 gccaatccga cgtcaattat cagcgtggta atttttgccg cattcctcgg cgtagctgcg
     5281 ctgaagctgc tgaaggatga tgcgccgaaa ggtgaacgcg tcttaaccgc tatcgatacc
     5341 ctgcaaagct gggtgatgaa actggttcgc ctggtcatgc agctgacccc ttacggcgtt
     5401 ctggcgctga tgaccaaagt ggttgcaggt tctaacctgc aagacatcat caaactggga
     5461 agtttcgttg tcgcgtccta cctcggtctt ctgattatgt ttgcagtgca tggccttctg
     5521 ctgggtatta atggcgtgag cccgctgaag tacttccgta aggtatggcc agtactgacg
     5581 tttgccttta ccagccgttc cagtgctgcg tctatcccac tgaatgtgga agcacaaacg
     5641 cgtcgactcg gtgttcctga atccatcgcc agtttcgccg cctctttcgg tgcaaccatt
     5701 ggtcagaacg gctgtgccgg tttgtatccg gcgatgctgg cggtgatggt tgcgcctacg
     5761 gttggcatta acccgatgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     5821 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     5881 cctgcgatgg gcctgccggt aacgctggtg gcattgttaa tctccgttga accgcttatc
     5941 gacatgggtc gtacggcgct aaacgttagt ggctcgatga ccgctggcac gctgaccagc
     6001 cagtggctga agcaaaccga taaagctatt ctggatagcg aagacgacgc cgaactggca
     6061 caccgttaat cctgatttgt tacgttaaac gcctgtcatc ccgacaggtg tttttttatt
     6121 cttcgacctc attttcctga cgaatctgat ttgcccaacg ctgggcggat tcaggcacag
     6181 taaacgccgg tgagcgcaga aatgactctc ccatcagtac aaacgcaaca tatttgccac
     6241 gcagcatcca gacatcacga aacgaatcca tctttatcgc atgttctggc ggcgcgggtt
     6301 ccgtgcgtgg gacatagcta ataatctggc ggttttgctg gcggagcggt ttcttcatta
     6361 ctggcttcac taaacgcata ttaaaaatca gaaaaactgt agtttagccg atttagcccc
     6421 tgtacgtccc gctttgcgtg tatttcataa caccgtttcc agaatagtct ccgaagcggg
     6481 atctggctga tggtctatag ttagagggtt ttttgaccaa aacagcggcc ctttcagtaa
     6541 taatttaagg agacgagttc aatgtcgcaa cataacgaaa agaacccaca tcagcaccag
     6601 tcaccactac acgattccag cgaagcgaaa ccggggatgg actcactggc acctgaagac
     6661 ggatctcatc gtccagcggc tgaaccaaca ccgcctggtg cacaacctac cgccccaggg
     6721 agcctgaaag cccctgatac gcgtaacgaa aaacttaatt ctctggaaga cgtacgcaaa
     6781 ggcagtgaaa attatgcgct gaccactaat cagggcgtgc gcatcgccga cgatcaaaac
     6841 tcactgcgtg ccggtagccg tggtccaact ctgctggaag attttattct gcgcgagaaa
     6901 atcactcact ttgaccatga gcgcatcccg gaacgtattg ttcatgcacg cggatcagca
     6961 gctcacggtt atttccagcc atataaaagc ttaagcgata tcaccaaagc agatttcctc
     7021 tcagatccga acaaaatcac cccagtattt gtacgtttct ctaccgttca gggtggtgct
     7081 ggctctgccg ataccgtgcg tgatatccgt ggctttgcca ccaagttcta taccgaagag
     7141 ggtatttttg acctcgttgg caataacacg ccaatcttct ttatccagga tgcgcataaa
     7201 ttccccgatt ttgttcatgc ggtaaaacca gaaccgcact gggcaattcc acaagggcaa
     7261 agcgcccacg atactttctg ggattatgtt tctctgcaac ctgaaactct gcacaacgtg
     7321 atgtgggcga tgtcggatcg cgggatcccg cgcagttacc gcaccatgga aggcttcggt
     7381 attcacacct tccgcctgat taatgccgaa gggaaggcaa cgttcgtacg tttccactgg
     7441 aaaccactgg caggtaaagc ctcactcgtt tgggatgaag cacaaaaact aaccggacgt
     7501 gacccggact tccaccgccg cgagttgtgg gaagccatcg aagcaggcga ttttccggaa
     7561 tacgaactgg gcttccagtt gattcctgag gaagatgaat ttaagttcga cttcgacctt
     7621 cttgatccaa ctaaacttat cccggaagaa ctggtgcccg ttcagcgtgt cggcaagatg
     7681 gtgctcaatc gcaatccgga taacttcttt gccgaaaacg aacaagcagc attccatcca
     7741 ggtcatattg ttcctggtct ggacttcacc aacgatcccc tgttgcaggg acgtttgttc
     7801 tcctataccg atacacaaat cagtcgtctt ggtgggccga atttccatga gattccgatt
     7861 aaccgcccga cctgccctta ccataatttc cagcgtgacg gcatgcatcg tatggggatc
     7921 gacactaacc cggcgaatta tgaaccgaac tcgatcaacg ataactggcc gcgcgaaaca
     7981 ccgccggggc cgaaacgcgg cggttttgaa tcataccagg agcgcgtgga aggcaataaa
     8041 gttcgcgagc gcagcccatc gtttggcgaa tattattccc atccgcgtct gttctggcta
     8101 agtcagacgc cattcgagca gcgccatatt gtcgatggtt tcagttttga gttaagcaaa
     8161 gtcgttcgtc cgtatattcg tgagcgcgtt gttgaccagc tggcacatat tgatctcact
     8221 ctggcccagg cggtggcgaa aaatctcggt atcgagctga cggacgacca gctgaatatc
     8281 accccgcctc cggacgtcaa cggtctgaaa aaggatccat ccttaagttt gtacgccatt
     8341 cctgacggtg atgtgaaagg tcgcgtggta gcgattttac ttaatgatga agtgagatcg
     8401 gcagaccttc tggccattct caaggcgctg aaggccaaag gcgttcatgc caaactgctc
     8461 tactcccgaa tgggtgaagt gactgcggat gacggtacgg tgttgcctat agccgctacc
     8521 tttgccggtg caccttcgct gacggtcgat gcggttattg tcccttgcgg caatatcgcg
     8581 gatatcgctg acaacggcga tgccaactac tacctgatgg aagcctacaa acaccttaaa
     8641 ccgattgcgc tggcaggaga cgcgcgcaag tttaaagcaa caatcaaggt cgctgaccag
     8701 ggtgaagaag ggattgtgga agctgacagc gccgacggta gttttatgga tgaactgtta
     8761 acgctgatgg cagcacaccg cgtgtggtca cgtattccta agattgacaa aattcctgcc
     8821 tgatgggagg cggcgcagtt gcgccgcctc aaatgattta catagtgcgc tttgtttatg
     8881 tcggatgcgg cgtgaacgcc ttatccggcc tacaaaactg tgcaaattca atatattgca
     8941 ggaaaaacgt aggcctgata agcgaagcgc atcaggcagt tttgcgtttg tcagcagtct
     9001 caagcggcgc agttacgccg cctttgtagg aattaatcgc cggatgcaag gttcacgccg
     9061 catctggcaa acatcctcac ttacacatcc cgataacacc ccaaccgata accacgctga
     9121 gcgatagcac ctttcaacga cgctgatgtc aacacatcca gctccgttaa ccgcgggaag
     9181 caataagcac tctggcgaat cgtattatcg ataaacgccg ggtggcacat cacttccaga
     9241 gagcgctcgc cgcgatgact ggagtcatca agcacttgta ggaacagtgt ctcgctaatc
     9301 tcttcaccat aaaacgcgct actgaatccc tgcgaactgc gcagattagc cggtaaatca
     9361 ccatcgttag atagcggctg acggtcgatg cgcaacgcaa tcccctcttc agccgcaaac
     9421 tttgccacaa tcgggaaaat ctgcgggaac atatgcacat gatgatggct atcaagatgc
     9481 gtaggtttgc gtccaaataa ctcaatgaaa cgcaaatact gactggcaag ctcctgagta
     9541 atttcttcca gcggtaaagc atcttcttct gccaactgcc agatccattt tcccagcaca
     9601 ccatcgcgag ttaaccccgg catagctgtc agtggcttgc ccatagtaag gacaaagtgc
     9661 atccctatgg ccagactcgg ttcatcaaga ctcaactgca ccgcatggtc aatagcctgc
     9721 ccattcacca gcgccgtcgt cgatgtgaca atcccattgc gacaggcctc gataatgccg
     9781 tagttctgac ctttgcttaa gccaaaatca tcggcattaa caatcagtaa gcgttccata
     9841 atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc agtcggcaaa atttggcaac
     9901 catttctcgt gcgccagaat catctcgcgt gccagcagct cagcatcgcg atcggaatgc
     9961 accaacggac taaggtttag ggccagtaac acatcgttaa actctccgct aagtgcggcg
    10021 ttgctggcag caatctcgaa gcctttaatg gtgtgaatca gccccatcac tttatcatcg
    10081 aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc ccagcgtaca ggtcatttct
    10141 accgcccagt ctgccggaat attatcaata tgcccatgat gcgggatatt aacgtaatgt
    10201 tcagcttgct tgtcgttgta gatagcgttg atcacttcgc acgctgcatc agagtaataa
    10261 gccccaccgc gctgttccag ttctttcggc ttaactttca actccgggtt tttatatagc
    10321 tcaaaaagtt gtttctcgac tttctgtact acctgcgctc gtgcgccgcc tttgtagtat
    10381 tcgcccattt caatagccag catctctttt tgcttgaagt aataaagcaa ataagaacac
    10441 ggcaacagat tcatagaacg aattaagcct tcgctaaatg gcagatcgaa aatattttta
    10501 acgccagatg cttttaactg cccggatgcc acaccatcaa gcaattcggc aaagcgtgat
    10561 ttgccattta ccagcacatc cttaatgaac accatatggt tgaggccgaa cagatcgata
    10621 gataaatcat cgctgtcttt cagcatcaga acatcgcgaa taaacatctt catgccgatc
    10681 ggaatattac acacgccgat aaagcgttta aatccggtat gacgataaac ggcttcagtg
    10741 accattcccg ccgggttagt gaagttaatc acccatgcat tcggacatag ttcttcgaca
    10801 tcttttacga tgtcaaaaat caccggaatg gtacgcagac ctttaaacag accgcccgca
    10861 ccgttagttt cctgaccaag ataaccatga cttaatggaa tacgttcatc cagttcgcgc
    10921 gccggtaatt ggccaacgcg cagttgggta gtaacgaaat cagcatcttt caatgcttcg
    10981 cggcgatcca gcgttttata aagcttcatc gggacgccag cgttatcaat catccgttgg
    11041 cagagatcaa aaataatatc cagtttcgct ttaccacctt cgacatccac cagccataat
    11101 tcgctgaccg gcaattcgtg ataacgctta ataaatcctt ccagtaactc cggggtatag
    11161 ctgctcccgc caccaatagt gacgactttt aatttctggc tcataatttc tcccttcagt
    11221 acagaatact gatatctggc atatctgccc cccggacata aataatccag caacaggaca
    11281 gatatgtgaa ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata
    11341 gctcttaggc gtaaaggatg ttaatttctt aaacgtttta ataaacaagc tcgggctact
    11401 ataaccggcc tcaaacgcaa tatccgtcac tgaatagttg gtcatttcca gttgtttttt
    11461 ggcaaaatta atacggattt cattaataat ctgcattggc gttttaccat agtatcgttg
    11521 agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc
    11581 cgattcacta aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg
    11641 tatatcatca atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt
    11701 aaccatctca acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt
    11761 ttccacatag gtgagaaaag cgttattggt ccggtatacc tgcgaagcga caaagcaata
    11821 aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat
    11881 acgcgtggca ccaaattcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa
    11941 atcgccccgt tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa
    12001 taccagagta aattcataat agtcgtgctg atgcagtccg ctgatactct cagttttatt
    12061 gtagataaac acatggaaat ttttgccatt aaacaactgc tgttctcggg cagtggcaat
    12121 ttccagcgcg ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa
    12181 gctcaattaa ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag
    12241 cgtggaccag caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt
    12301 tcgtctgtac cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggtttttg
    12361 ctgcggcaaa atcgccctgc ttcgcctgtt tcagtgccgc atacgccagg ctgcgcgctt
    12421 gtccggagtt gatgataagc cccatcacca cttcttccag ctcttcaact tccgtttgcg
    12481 tatcgggaat attatcgaga tccatcatac atcgttcctc tctattctta ccggcacgat
    12541 tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg atatcttctt cgctctcttc
    12601 tttatcaatc gcgttttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt
    12661 tgcgatacca aggttgaaga gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa
    12721 gaaggctccc agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaataat
    12781 gcccatgtag tacgctgcga gggtgattgc cgccagaatc ggttgtacca gtacaaacgg
    12841 gataaacatc accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg
    12901 gaagatgccg gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc
    12961 gataaagata gccaggatca gacctaaagt cgcaccactg cccccaagga aaataaagga
    13021 gtccagcatc ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac
    13081 ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc caaggcgtca taatgccgtt
    13141 gtccagtgcg gtcagcgcca gcgcaccatg aataccgaag aaccagagca gtggaacaaa
    13201 gatcacatag gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc
    13261 cataataatc tgatggaagt tggtgcccca ggtattcaac gtccaggcaa taatccccat
    13321 cacggaaaga ataataaagc cgggaattaa agccgagaag gaacgcgata ctgaagcagg
    13381 tacgctatcg ggcaatttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc
    13441 caccaccagg ccaataataa tcccggagat gatattcgcc ccacctaacc agtttgcgcc
    13501 aaccgcatag gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa
    13561 caacccggcc gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa
    13621 cggtgccatt aaagacatta tccctaatgt tccgttatat acgttgccgc caataccttt
    13681 cagaccatta agtgtttcaa tggttgaggc atccaggcga atacctaagg aataaaaaaa
    13741 cgacccctcc ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag
    13801 ggttaacggc attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc
    13861 tattttaact gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga
    13921 aaaataccct taaaaaccgc aatttaaata ttgcggtatt gatttatgaa ataactcttt
    13981 gacgggaaaa tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc
    14041 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcgaccgg
    14101 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
    14161 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
    14221 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga
    14281 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
    14341 gattatctgt cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt
    14401 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
    14461 agtatttctt tgacctgata aattgtctct ccttgacctg cggttatgac cctcttcaca
    14521 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat
    14581 ggccggtttc cgttgtaact caagctttca ggcgaagtga ttacttcgca gcctgtggat
    14641 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
    14701 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
    14761 taccacgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
    14821 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg
    14881 tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
    14941 ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga
    15001 atacattggg cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg
    15061 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta
    15121 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
    15181 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt
    15241 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
    15301 cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct
    15361 gcgccaggaa actggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
    15421 tcaggctgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
    15481 aaccgttaat atcaagggcg cggtattggc cagcgaacag gcgctgcgcg aagcgggtat
    15541 tgaattaagt gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
    15601 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
    15661 taccggattc ttcactaaat atggtgacgg cggtacggac attaatccac tgtatcgtct
    15721 taacaaacgt cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa
    15781 gaaagcgcca acggccgatc tggaagatga tcgcccttct ctaccagatg aagtggcact
    15841 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt
    15901 cgccagaaca atagagaact ggtatctgaa aactgaacat aaacgccgtc cgccaattac
    15961 cgttttcgat gatttctgga agaagtaata atttgcacat attggtttgt gcgaaaaaga
    16021 gtactttgtt cacgccggat gcggcgtgaa cgccttattc gccccacaaa actatgcaaa
    16081 ttcaatatat tgcatgagtc gaataggcct gacaggcgta gcacgtctga cggtgtaacc
    16141 tttgtcatca acccgcctct tttttaatcg ctccccgcct gttacactgg atagataacc
    16201 agcattcgga gtcaactgtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
    16261 cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac
    16321 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagca
    16381 ttaacattcg cagccgcgtc ctttctcatt tacgtacccc ggatgaagcc gccatgctac
    16441 ggcaatcccg acgcatcagc tggatatgta ccgccggtga aatcggcgct ctactccttg
    16501 aagcgcgatt aatcaaagag cagcagccgc tgtttaataa acgcttgcgc cgcaatcgcc
    16561 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
    16621 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
    16681 cattgcagac catcgctgat gaacaaaaac tttgttatgg tctgttggga ctggagccgt
    16741 taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
    16801 gtaaagagag ccatgacgat catgcgctac gtttgcgcca gtctctggaa cgtttacgag
    16861 tagtttgttg gccgtggaaa ggtgcggtgg cgctgaaaga acagcacccg gaaatgactc
    16921 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
    16981 caatgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
    17041 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
    17101 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
    17161 atcgaaccag cacgcgccct ggtcggtagt gagcagttta tcgcccaatt gccacgcccc
    17221 gttaatgaca aacactaccc cgccacgtga gccaaaggta gtaaacgtac gttcggcaat
    17281 tctgactttc gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg
    17341 ccctgccgtc agtttcgctt ttaccacctg gtccgccgca aaggcaaaag gctgtaacgg
    17401 ttttaaggta tggttaaagc ggtctgtgct ttcaaggaac atctcgccgc cttccagcaa
    17461 cgtcactatc ctttccatac ctggaaacaa agaaaactcc ccattcgccg cgatggatgc
    17521 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
    17581 ttcaccggca gcatttcgcc acagattcac cgacatttta cgcatatcaa agtattccat
    17641 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
    17701 caactcgggt gatttacgtt agtggcgatc aggctttttt tgcatctgtt tgagaacaaa
    17761 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
    17821 cttacgtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg
    17881 gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca
    17941 agatgttgta aatcttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt
    18001 cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga
    18061 tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt catctggtca cgctggcctt
    18121 tcatgatttc gcggatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca
    18181 tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg
    18241 ctgaggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg
    18301 caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac
    18361 ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt
    18421 aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga
    18481 aaaaaagaaa atttgatgga gagtaatgag agaatattac aacacgatga ttttgcagag
    18541 attatgaaga actataccgg atgactggtg ataaataaag caaataacca ggattaatct
    18601 gtattaattt ataagaaagt aacttaatac ccgcagaatg atttctgcgg gtaagtatta
    18661 gcttattttt tcgagcatta atcccgcgcg taatcccaac gctaccaaag gattagggaa
    18721 taacacatac tctacatcat gggttacggt aaaacgttcc tccccgtcct gcgccagcaa
    18781 tgttcctttc tcaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga
    18841 cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggcg ttctcacgat
    18901 accgacactc tcaccagata gcagcgcagc aattgcgctg gcagtgactg caaactggcg
    18961 aagatcgttt tgtccaaagg gcaacgcttt gccgagttcc agcgtacagg ccagcgcgcc
    19021 aaaatgtctg gcgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagtgc
    19081 ctccagaccc gccgcaccca gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg
    19141 cggtaataca ccgaaccgcg gatgcaagga gccacgaatt gcggtatgga gatcaaggtg
    19201 ccagcgcaca gattctttgc cctggtcata aaaatcttcc aggcactgct ccagttcgcg
    19261 cgcccgacag gtttccccgc tttcagcaaa tagctgccaa cgaccgccaa acattcgatt
    19321 catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac
    19381 cagtaaccgc caacgtaacg ggatctcacc gtgagatatc gcgccaagca acgcgtccag
    19441 catttccaca ggtgccgtct cattaccgtg gatccccgct gaaatcacca gcgcgccttg
    19501 cggtggcgtt aatggcgtca gttccagcac gccatcgccc agccagcgcc agcgaacgcc
    19561 gttgatttct cgctcggtga taaccgtttt tttacccgtt aaggtcagag caagaaaatt
    19621 atccatttcc ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc
    19681 tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag
    19741 cagtaaggcg atcgcggtag taacgatcca cccagtcatt gagcgcatta aacagcgtat
    19801 cgttcatcat caccgccgga ttcactgccc ggcgttcttc ttctgtcaat accacgcgca
    19861 accgcaggca cgccgggcct ccgccattcg ccatgctttc acgtaaatca aagactttta
    19921 gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt
    19981 gttcccgaca ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcagcagtt
    20041 ggctgttaaa cagatatgta gacaccgcat ctgacacgga aacctgagtc gccggaactt
    20101 ctatcgccat aaaaccgttc acccgcgcac gcaggcttgc cagtaactgt gactggcgag
    20161 cgaacgcctg ttgatggcaa aacagcacct ggcggttact cacggcaatc acgtcattat
    20221 gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgttggggat
    20281 tcacctgatt cagccttgcc accgcctcgc tggcttcgcg agtctgccgc gccggataac
    20341 gggaaggccg ggtatcattg ccttcttctc gcccgtagac aaaaagttgc atacccggtt
    20401 caccgtaatg accgccgaga cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta
    20461 cctgcggcaa cgccgaatgg acactaaatt tctcttcgtc gttaaaaatc gcttttaaca
    20521 acgattcggt gacgggggct tccagcgaac ggtgaaattt attgttcagg ttggcaacgg
    20581 tgagatgcac tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta
    20641 cccacattgg cgaagcggag ctgacgctgg aaagccagtg cggtgcctgg cgggcaacct
    20701 tttccagtac ctgctcatcg ctgccgctga atcccaactg acgcagcacc ggaataaacg
    20761 gacgctcgtg cggcgggatc accgcctggg ggaatcccgc gtcggcaagg gctttcattt
    20821 tcagtaagcc ctgcttcgcc gccagtcgcg ggttagagac ctgaaaacgg tgacgggtgg
    20881 aggcttcatt accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga
    20941 aattgatttc ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc cggggttaag
    21001 cgttgcgggc aatgttaacg agtccgactc cagacttgcc atcggccagg cacaataatc
    21061 tgcggcatac caggcgctgg ggcgatggtt accggaagcg ccaatgccgc cgaatggcgc
    21121 ggtactggca gcaccggtaa gcggtttgtt ccagttaaca atccccgccc gtgcctccag
    21181 caacagttga tcgaattttt cccgctcggg ggaaaccaga ccgcaagaga ggccgaagcg
    21241 agtgttattc gccattcgaa tcgcttcatc gaaattgtca taacgccaga cgcgcaataa
    21301 cggcccaaac acttcttcat ccggcacgcc agtaacgcct gtcatttcaa tgatccccgg
    21361 cgtcagcaac gatgtccctg cttgtaataa gcgcggcgca agcagggtcc gtccgcccat
    21421 cgcctccagt tcctgccagg cagtaaccac ctgctgtgcg gcctgctcag aaatcagccc
    21481 gccaataaac ggctgcggtt cgtcatccca acggcccggc gttaaccgct ggctgacggc
    21541 aaccaggcga gcaagaaatg catcgccctg cgttccactt ttcagcaata aacggcgggc
    21601 gcaggtgcag cgttgcccgg cagtgacaaa cgccgactga atggtcagat ggacagcggc
    21661 atcgatatcc gccacctcat cgataattag cgggttatta ccgcccatct caagggcgag
    21721 aattttctcc ggctgaccgg aaagctggcg atgcagctgg tagccggtat tggcgctgcc
    21781 ggtaaacagc aagccgtcga gatcctccag cgcactcagc gcctgacccg tttcacgccc
    21841 gccctgcacc aggttcagca cgcccggcgg caagccagcc tgctgccata aacgcattac
    21901 cgcttcgcca ctccacggtg tcagttcgct gggtttaaag ataatggtgt tacctgccag
    21961 caatgccgga acgatatgtc cgttcggtaa atgaccgggg aaattatacg gcccaaacac
    22021 cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgcg ccgtccggca tttcactacg
    22081 ctgctcgccg gtacgaacgt gatacgcctt aattgatatc gcgattttat tgatcatcgc
    22141 catcacttcg gttgccgctt cccagcgcgg cttacccgtt tctctggcaa taatcgcggt
    22201 taattcggct ttattgcttt ccagcaatcc ggcaaagcgt tcaactctta cctgacgatc
    22261 gccaaaggag agacgcgccc agcgcggaaa cgccgcacgg gcagcccgac aagcctgccc
    22321 gacctgagcg gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg
    22381 cttcacacgc gatgcgccct ggcccgtaac ccagtcacca ttgatccata aagtcatgct
    22441 gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat ccccggcgtg gcatttcagg
    22501 gcatccagtt gtgcggcggt taatatcaaa cgctcggttg ccggatcggt acgcaccagc
    22561 accacgcgga aatggtgata attttcattg gcggccaggc aggccgggaa atcgccctgc
    22621 gcaggctgcc cttctgccac ttccaccagc cgacttttgc ggatggcgcg cacgcggtcg
    22681 atgtcacact caagcgtcgg cccgccgtca aagatgtcga tatagttgcg gtagcgaaaa
    22741 ccttctttct ccagcaccgc gcgggcgggc gcggtttgcg gatgtacctg gccgataacg
    22801 tcctgcgctt cctgggataa aaagtgggta tagatcggat gtttcggcat cagttccgca
    22861 ataaatgcct tttgcccggt gccacagaga aaatcggcgc ggctaaaatc catcgaaaag
    22921 aagcgtttac cgaggctttg ccagaacggc gaatagccat gttcgtcaat caccccgcgc
    22981 atttcggcga ccactttgtc attaaacttg tcgcgaaagg ccgccataaa cataaagcgc
    23041 gatttcgaca gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg
    23101 cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca gcgtcggcaa cgcgttatag
    23161 acattcagct cttttgaggc gtgaaccagc gtgccgacgc gatagttgta ccagggatcg
    23221 ttcagcccaa ccgccacctc aatggcgcaa atccccgcca cagtgcctgt ttcgctctct
    23281 tccagtacaa acacataacc ctgctcgctt ttgggcagtt cgccctgcca ggttttgatg
    23341 gccctttcga tacgcgctga aagcgtggct tcattagcag ggagcgacgt caggccgccg
    23401 cccgttttgc tggcaagctg catcagcgcc gcaacgtctg agcgctcaac gggacggatg
    23461 accatcatga tgaacctccg ctcacaaagt gttcacaagc cgccgcaaaa cgatccagcc
    23521 cggtcgtcac ctcttcttcg ctgacattga gcgctggcgc aaaacgcact acgttgccac
    23581 cggcaatcag caccattacg cccgctttcg ccgcttcctg agaaatttgt ttcgcttgcc
    23641 cggcgtaatc ggcattcagc acacagccaa tcagcagacc taatccgcga atttcactga
    23701 acaaaccgca gcggtgatta atggtattaa gacgctcaac aaaccagtcg tgacgctgtt
    23761 taacgccatt aagcatctct ggcgtgttga tgagatccag cactttacct gccaccgccg
    23821 aggccagcgg attaccgcca taagtggtac catgtgtgcc cacggtcatc acgctggcgc
    23881 attcttcggt tgccaacaga gcgccaacgg ggaaaccgcc gcccagtgct ttggcggtag
    23941 ttaacagatc gggcgtcacg ccgtagtgca tataggcata cagttccccg gtgcgcccga
    24001 cgccggtttg tacttcatca aaaatcaaca gcgcattgtg gcggtcacac aattcacgca
    24061 gaccttgtaa aaatgcgttg cttgctggca ccacaccgcc ttccccctgg atgggttcga
    24121 caatcaccgc acaggtagag tcgtcaatca gcgcgctggc agagttaata tcgttatatg
    24181 cggcatgacg aatatccggc ggcagtggcg caaaatcctg tgaataggct ggctgaccgc
    24241 ccgcactgac ggtaaacagc gtgcgaccat gaaacgcatt tttaaacgcc acgataccgc
    24301 tcttatggct gccgtagcgg tcgtgagcga atttacgcgc cagttttagc gccgcttcgt
    24361 tggcttccgc accggagtta caaaagaaga cgcgataggc aaacgtggcg tcgatcaatt
    24421 ttttcgccag tcgcagtacc ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac
    24481 tcgcctgttc gttcagcgcc tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg
    24541 caatgccacc cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt
    24601 cgccacgtac cggtataaac ggtgccggag cgtaaacagg tatcatccat tcatcaaagt
    24661 tttcacgcgt aattggctga gacatagcga cctctacagt aaataattcg ttatttatat
    24721 gttaataata agtaatgttt gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg
    24781 ataaaagtgc ataaacggcg gaggctaact ggaaatcaag gagttataac caaaccatat
    24841 gcatttaaag tgcatataaa gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa
    24901 aacgcaatgt tatgcagaag taaaatataa ttctggaatt gtgatcattg acgaaattta
    24961 ctggaaatta ctgcgccatt ctgacgcaac gcgcaccaaa agcgggcatt ttttgcgcca
    25021 tcgttgacat cattaacaac catcgatcaa atcacttaac aacaggcggt acgcaaagcg
    25081 aaattctgct accatccacg cactctttat ctgaataaat ggcagcgact atgaaatttg
    25141 tctcttttaa tatcaacggc ctgcgcgcca gacctcacca gcttgaagcc atcgtcgaaa
    25201 agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat atgtttccgc
    25261 tcgaagaggt ggcgaagctc ggctacaacg tgttttatca cggacagaaa ggccattatg
    25321 gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg
    25381 acgaagaggc gcaacggcgg attattatgg cggaaatccc ctcgccgctg ggcaatgtca
    25441 ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga ccatccgata aaattcccgg
    25501 caaaggcgca gttttatcag aatctgcaaa actatctgga aaccgaactc aagcgtgaaa
    25561 atccggtgct gattatgggc gatatgaata tcagtcctgg cgacctggat attggcattg
    25621 gcgaagagaa ccgtaagcgc tggctgcgta ccggaaaatg ttctttcctt ccggaggaac
    25681 gcgaatggat ggagagactg atgagctggg gattggttga taccttccgc catgcgaatc
    25741 cgcaaacagc caatcgtttc tcatggtttg attaccgctc aaaaggtttt gacgataacc
    25801 gtggtctgcg catcgacctg cttctcgcca gccaaccgct ggcagaatgt tgcgtagaaa
    25861 ccggcatcga ctatgaaatc cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg
    25921 cgaccttccg ccgctaattt agctgttctc ctggctcaaa ctgggtcagg agaattaacc
    25981 ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc tgccgtcctt tgttataccg
    26041 tcactgcgtt tttagttgtc tgaccacttc tctattatca agtttgatat aggaaactcc
    26101 acgatgaacg ctgagcgtaa atttcttttt gcctgtctta tttttgcgct ggtcatttac
    26161 gctttccacg ctttcggttt attcgagctg ctcaccgatt tacctcactt actgacactc
    26221 attcgccaga gcggattgtt cggttatagc ctctatattc tgttattcat cattgccacc
    26281 ctctttctgt taccaggaag catactggtg atcgctggag gaatagtttt tggcccgctc
    26341 ttagggacac tactatcatt aattgccgcc acgctggcct cctcgtgctc attcctgctg
    26401 gcgcgctggc tgggacgtga tttactgctg aaatacgttg gccatagcca tacctttcag
    26461 gccattgaaa aaggcattgc gcgtaacggt attgattttc ttattctgac ccgcttaatc
    26521 ccgttgtttc cttacaatat tcaaaattac gcttacggat taaccacaat cgccttctgg
    26581 ccttataccc ttatttcggc actcacaacc ctgcccggta ttgttattta taccgtgatg
    26641 gcaagcgatc tcgccaatga aggcattacg ctgcgcttta ttttacaact ctgtctggcg
    26701 ggcctggcac tgtttattct cgtccagctc gcaaaactct acgcccgaca caaacatgtg
    26761 gatctgtctg cttcgcgccg cagcccactt tctcacccca aaaatgaagg atagaacgat
    26821 gttgcaacat tattcagtgt catggaaaaa aggactggct gcactctgct tactggctgt
    26881 tgccgggctt agcggctgcg atcaaaaaga gaatgcggct gcaaaagtgg aatacgacgg
    26941 actttcgaac agccaaccgc tgcgtgtcga tgccaataac catacggtaa ccatgctggt
    27001 gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt ttaaagatgg
    27061 ctccaacgga cataaatcgc tgtttatggg ttatgcgacc ccgaaagcct tttatgaagc
    27121 cctgaaagag gcaggtggta caccgggcga aaacatgacg atggataata aagaaacgac
    27181 tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg caaggggcgg caaaagcgta
    27241 ttccttcgat gaagtgattg ttgacagtaa tggaaagaaa ctggacatgc gctttggcgg
    27301 taatttaacg gcagcagaag agaagaaaac gggctgcctg gtgtgtctgg atagctgccc
    27361 ggtcggcatc gtcagcaatg caacatacac ttatggtgcg gttgaaaaac gaggtgaagt
    27421 taaattcaaa ggcaatgcct cggttctccc ggcggataac acgctggcaa cggttacctt
    27481 taaaatcacc gaataaagca ggataaagga tgatgaagat gcaatcgcga aaaatctggt
    27541 actaccgtat aaccctcatc atcctgttgt tcgctatgct gctggcatgg gcgctgcttc
    27601 ccggcgtcca tgagtttatc aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg
    27661 gtatagaacg ctttattcag tcttacggcg cactggcggc agttgtctcg ttcttgttga
    27721 tgattttaca ggccattgct gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt
    27781 cgctgtttgg cgcgttctgg ggcggcttgc tgtcgtggac cagttcgatg gccggcgcgg
    27841 cgctgtgctt ttttatcgcc agagtgatgg gccgcgaagt ggtggaaaag ttaacgggta
    27901 aaacggtact caatagtatg gacggctttt ttacccgcta tggtaaacac accattctgg
    27961 tatgccgatt attgcctttt gtgccttttg atcccgtaag ctatgctgcc ggtttgactt
    28021 caatacgttt tcgctcgttt tttatcgcca ccgggcttgg tcagttacca gcgactattg
    28081 tttattcctg ggcgggcagc atgttaacag gcggtacttt ctggtttgtc accggactgt
    28141 ttattctgtt tgccctgacc gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc
    28201 agaagaggaa tgcctgatgg gtttaccgcc gcttagcaaa attcctttca ttttacgtcc
    28261 gcaggcgtgg ctgcatcgtc gccattacgg cgaggtgcta agcccgattc gctggtgggg
    28321 gcggatcccg tttatctttt atctggtgtc gatatttgtc ggctggctgg agcgcaaacg
    28381 ctcaccgctg gatccggtag tgcgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct
    28441 gtgtgagttt tgcgtggata tcaccagtat gaaagtcgcc gagcgcactg gcagcaccga
    28501 taaactgctg gcagtggctg actggcggca aagcccgctc tttagcgatg aagaacggct
    28561 ggcgctggag tacgcagaag ccgcaagcgt aacgccgcca acggtcgatg atgccctgcg
    28621 cgcccgactg gttacgcatt ttgacgctca ggcgctcacc gaactgacgg cattgatcgg
    28681 actgcaaaac ctgtcagccc gttttaattc tgccatggac attcccgctc aggggctgtg
    28741 ccgtattcct gaaaaacgtt cttaaggaga gatgatgcgc cattgtgtgt ggttgctggg
    28801 attgttatcg ctgttttctt tggcaacaca tgccagtgac tggcaggaaa tcaaaaacga
    28861 cgccaaaggg caaaccgtct ggtttaacgc ctggggcggc gataccgcaa ttaaccgcta
    28921 tctcgactgg gttagcggcg agatgaaaac ccattacgat ataaacctga agattgttca
    28981 tctcgcggat gccgcagacg cggtgaagcg cattcagacc gaagctgctg ccggacgcaa
    29041 aacaggcggt tcggtggatc tgctctgggt gaacggcgaa aacttccgca ccttaaaaga
    29101 ggctaattta ctgcaaaccg actgggcaga gactctgccc aactggcgct atgtcgacac
    29161 acagcttccg gtgcgggaag atttttcagt gcctacagaa ggggctgaat cgccctgggg
    29221 cggcgcacaa ctgacgttta tcgcccgccg cgatgttacg ccacagccac cacaaacgcc
    29281 gcaagcctta ctggagtttg ctaaagccaa tcccggcacg gttacctatc cgcgcccacc
    29341 ggactttacc ggcacggcgt ttcttgaaca gttgctgatt atgttgacac ccgatcccgc
    29401 cgcattaaaa gaagcgccgg acgatgcgac tttcgcccgt gtcactgcgc ccttgtggca
    29461 atttcttgat gcgctgcatc cgtatttatg gcgcgaagga aaggatttcc cgccttcgcc
    29521 cgcgcggatg gatgctctgc tgaaagctaa cacattgcgc ctgtcgctga cctttaatcc
    29581 tgctcatgcg cagcaaaaaa tcgccagcgg cgatttaccc gccagcagtt acagctttgg
    29641 ctttagcgag gggatgattg gcaacgtcca tttcgtcacc attcccgcca acgcgaatgc
    29701 cagtgctgcg gcgaaggtag ttgccaattt cctgctctca cccgatgcac aactgcgtaa
    29761 agcagatccc gctgtctggg gcgatccttc tgttctcgat ccgcaaaaac tgcctgacgg
    29821 gcagcgcgaa tcattgcaat cgagaatgcc gcaggatctg ccgccggtac tggctgaacc
    29881 gcacgcaggt tgggtaaatg cgctggaaca agaatggcta caccgttacg gtacgcatta
    29941 atctttttgc tgtgggcgat gatggcggtg atttatgcac cgctgatccc ggcagttctc
    30001 acgctgatat cgcctgcttt gtcgttgacg cactggcagg cgttatttgc cgatccacag
    30061 ttaccgcagg cattactggc aacgctggtg tcgacaacca ttgcggcggt cggggcgttg
    30121 ttgattgccc tgctggtgat tgtagcgctg tggccagggc cgaaatggca gcgtatgtgc
    30181 gcccgtctgc cgtggctgct cgccattccc catgtggctt ttgccaccag cgcccttctg
    30241 ctctttgctg acggagggct gctttatgac tatttcccgt atttcactcc gccaatggac
    30301 aaattgggta ttgggctggg cctcaccctg gcagtgaaag aaagcgcatt tctgctgtgg
    30361 atcttagcgg cagtattgag cgaaaaacgg ctgttgcagc aactcattgt gctggattcg
    30421 ctgggctaca gccgctggca atgcctgaac tggctgctgt tgccctctgt cgcgcctgcg
    30481 ctggcaatgg cgatgctggc gattgtggcc tggtcgctgt cggtcgtgga tgtggcgatt
    30541 atccttgggc caggtaatcc cccgacgctg gcagtaatta gctggcagtg gttaactcag
    30601 ggcgacgccg atcaacaagc gaaaggcgca ctcgccagcc tgctgctgat gctgttactc
    30661 gccgcctacg ttttgctggg ctatctgcta tggcgtagct ggaggcgtac tattccccgc
    30721 gtagatggcg ttcgtaagcc tgccacgcct ttattgcctg gcattactct ggcgagtttt
    30781 ttacccttga ccggtgtgct gtgtgtggtt ctgttggcga tcctcgcgga tcagtcgacg
    30841 atcaatagtg aagcgctcat caatagcctg acgatgggac ttaccgcgac gtttatcgct
    30901 ttaatcctga tactggcgtg gctggaatgg ggatcgtcac gtcggcattt ctggctatgg
    30961 ttgcccattt tattacccgc tctgccactg gtggcgggcc agtacacgct ggcgctatgg
    31021 ctaaacctag atggaagctg gacggcggtg gtctgggggt atctgctgtg ggtaatgccg
    31081 tggatgctgt ttatcctgca acccgcctgg cagcgcattg attcacggtt aattttgatt
    31141 gcgcaaacac tgggctggtc gcggtccaaa atcttctttt acgtgaaatg tccgcttatg
    31201 ctacgcccta cgctgattgc cttcgcggtg ggattttcag tcagtattgc acagtatatg
    31261 ccgacgctgt ggctgggcgc ggggcgtttt ccgacgctca ccactgaagc ggtggcatta
    31321 agcagcggcg gcagcaacgg cattctcgcc gcccaggctt tatggcaact gctattaccg
    31381 cttattattt ttgccctgac cgcgttagtc gcaaaatggg taggttatgt cagacaagga
    31441 ctccgctaat gctctgcgtg aaaaatgttt cgctacgttt accagaaagc cgcttgctga
    31501 caaacgttaa ctttacggtg gataaaggtg acattgtcac gttaatgggg ccgtctggct
    31561 gtggaaaatc cactctgttt tcatggatga ttggtggact ggccggacag ttttcttgta
    31621 caggggagct atggctcaat gagcaacgga ttgacatgct acccaccgca cagcgtcaga
    31681 ttggcattct tttccaggat gcactgttat ttgaccagtt cagcgtcggg caaaatttac
    31741 tgctggcgct accggcgaca cttaaaggga atgcccgacg taatgccgtg aatgatgcac
    31801 ttgagcgttc aggccttgac ggagctttcc atcaggatcc tgctactttg tctggcggtc
    31861 agcgagcgcg cgttgctctg ctacgcgccc ttctcgccca gccaaaagcg ttactcctgg
    31921 atgagccatt cagccgtcta gatgtggctc tgcgcgataa ttttcgccag tgggtgttca
    31981 gcgaagttcg cgcccaggcg atccccgtcg ttcaggtaac gcacgatctc caggatgttc
    32041 ctgctgatag ttctgttctg gatatggcgc agtggtcaga aaattacaac aaactgcgat
    32101 aacgcaaagt ttttctcaat gcgtcagttc agaatggcgc actcaaaact acaatgtcgg
    32161 gattttcgat gaaacgtgtt tctcaaatga ccgcgctggc aatggcttta gggctggctt
    32221 gcgcttcttc gtgggccgct gaactggcga agcctcttac acttgaccag cttcaacaac
    32281 aaaatggcaa agcgatagat actcgcccca gcgcgtttta taacggctgg ccacaaacct
    32341 taaatggccc ttctggtcat gaacctgccg ccttaaacct ctctgctaac tggctcgaca
    32401 aaatgagcac cgaacagctc aacgagtgga tcaagcaaca taacctgaaa gccgatgctc
    32461 cggtggcgct gtacggtaat gacaaagatg tcgacgccgt caaaaagcga ctgcaaaaag
    32521 caggttttac gcatatctcc attctgagtg acgcgctaag cgaaccttcc cgtctgcaaa
    32581 aactgccgca ttttgagcag ctggtttatc cgcaatggct gcacgacctg caacaaggta
    32641 aagaggttac ggcgaaacct gccggtgact ggaaagtcat tgaagcggcc tggggcgctc
    32701 ctaagcttta ccttatcagc catattcccg gcgctgacta catcgatacc aacgaagtgg
    32761 aaagcgaacc gctgtggaac aaagtttctg atgaacaact aaaagcgatg ctggcaaaac
    32821 acggcattcg ccatgacacc acggtcattc tgtacgggcg tgacgtatac gctgcagcgc
    32881 gtgtggcaca aattatgctc tacgctggcg tgaaagatgt ccgcctgctg gacggcggct
    32941 ggcaaacctg gtccgacgcg ggactgcccg ttgagcgcgg aacgccaccg aaagtgaaag
    33001 cggaaccgga tttcggcgtg aagatcccgg cacaaccgca actgatgctt gatattgaac
    33061 aagcgcgtgg actgctgcat cgccaggatg catcgctggt gagcattcgt tcgtggccag
    33121 aatttatcgg tacgaccagc ggttacagct atattaaacc aaaaggtgaa atagccggag
    33181 cacgttgggg acacgctggt agcgactcga cgcatatgga agatttccat aacccggatg
    33241 gcaccatgcg tagcgccgat gatattaccg ctatgtggaa agcatggaat atcaagccag
    33301 atcagcaagt ttcattctac tgcggcaccg gctggcgcgc gtcagaaacc tttatgtacg
    33361 cacgcgcaat gggctggaat aacgtctccg tttatgacgg tggctggtac gaatggagca
    33421 gcgatccaaa aaatccggta gcaaccggtg aacgcggccc ggacagcagt aaataacatt
    33481 gcaattactg acgctggagc gacttcagcg tcagataccc actccacacc cgcgtaaagg
    33541 tcgtcatcca gcacaacgca ccaaataccc acgcaaacca cggaaaacag gccggaaaga
    33601 ggcaactcag aacaaacagc aaaatcgtct cgctgccttc ggttaagccg cccagataat
    33661 aaaacgattt atgcgcataa ccgggattat caatctgatg tttcgccgcc agcgcagcaa
    33721 aagcgagaaa actgctgccc gtgccgataa acgcaaacaa cagccagcct ccggccagcg
    33781 cgttttgttc tggtgcggcg agaataaaac caaacggcac cagcgcgtaa aagagaaaat
    33841 cgagagaaat atcgagaaag ccgcccgcgt cggtaagtcc tctgcgccgc gccagcgcac
    33901 cgtccagacc atcaagcagc ctgttcaaca aaatgacgat tagcgccgcc agataccagc
    33961 ccagcgccag aaacggcagt gccagcacgc cgatggcaaa tcccactaat gttaaaccat
    34021 ctggcgtgat gccgggttta tccagcacct tcacacactg atgcaacaaa ggtttaattc
    34081 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tccgattgag
    34141 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
    34201 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
    34261 gcccagagcg atcaggcggg attatgggag tttgccggtg gtaaagtcga gccggatgaa
    34321 agccagcggc aggcgctggt gcgtgagtta aacgaagaac tgggcatcga agcaactgtt
    34381 ggtgactatg ttgccagcca tcagcgagaa gtttcggggc ggattatcca tcttcatgcc
    34441 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
    34501 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc attattagag
    34561 gcgtttatgg ctttacgcgc cgccagacca gcggattagt gctaaggttt ttgtcatcct
    34621 gttggcattg cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
    34681 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
    34741 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattattg
    34801 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
    34861 ctctgctact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
    34921 gttatgagat tactctcgtt attaatttgc tttcctgggt catttttttc ttgcttaccg
    34981 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
    35041 taatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
    35101 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
    35161 aagtaatgac cacactctgg ccttttcttg aacaaaatcc caaatatcgt cagatgtcat
    35221 tactggagcg tctggttgaa ccggagcgcg tgatccaatt tcgcgtagta tgggttgatg
    35281 atcgcaacca ggtgcaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
    35341 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ctccattctc aaattcctcg
    35401 gcttcgaaca aaccttcaaa aatgccctga ctactctacc gatgggcggt ggtaaaggcg
    35461 gcagcgattt cgacccgaaa gggaaaagcg aaggcgaagt tatgcgtttt tgccaggcgc
    35521 tgatgactga actgtatcgc cacctgggcg cggatactga cgttccggca ggtgatatcg
    35581 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
    35641 ccgcctgcgt cttcaccggt aagggccttt catttggcgg cagtcttatt cgcccggaag
    35701 ctaccggcta cggtctggtt tatttcaccg aagcaatgct aaaacgccac ggtatgggtt
    35761 ttgaagggat gcgagtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
    35821 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
    35881 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
    35941 atggtcgagt ggctgattac gccaaagaat ttggtctggt ctatctcgaa ggccaacaac
    36001 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
    36061 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgccgaaggg gcaaatatgc
    36121 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
    36181 aagcggctaa tgctggcggt gtagcaacat cgggcctgga aatggcgcaa aacgctgcgc
    36241 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
    36301 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggggcga
    36361 acattgccgg ttttgtgaag gttgcggatg cgatgttaag ccagggcgtg atttaagaca
    36421 attgtgccgg atacagcgca aacgccttat ccggccataa aaatcttcag tatcagatgg
    36481 ttgaaagaac ctttatatta tcactccatt gcccgcctct tctgatggcg ggcttttttt
    36541 cggcgctctc ttgcgtggcg aatccttttt cttcccttct ccaccgccgg gagccattat
    36601 tccccgaaac tgccttaccg acgtgcgctt tgcctgatca atcaactggt acaatgtccc
    36661 caccagcggc tgcataaagt cctgatagcg acactgcttt tcgctgattt gcgtcagcac
    36721 cgattcccag tgcgcggtca tgtccggtcg cgtcgccatt tctggcagcg aatggaatag
    36781 cgcttttccg gcgtcggtgg agtggatata gcgccctttt ttggtcagga agcctcgctt
    36841 gaacagcagt tcaataatcc cggcgcgagt cgcttctgtc cccagaccat cggtcgcacg
    36901 aaggatcttt ttcagatctt tgtcctgcac aaaacgtgct atcccggtca tcgctgaaag
    36961 cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc cgctctacca cttcaccttt
    37021 ttcacacagc aactcatcgc ctttcgccac cacaggcagc ggcgtgccgt cgttttcttc
    37081 atcgcgctct ttgctgccta acagtgtgcg ccagcctgct tcagcaagaa aacgggcttt
    37141 agcgacaaat ttgcctttgg caatgtccag ttcgataaca cacttgcgga acaccgcatc
    37201 cgggcagaac tgcatcaaat actgacgggc aatcaggtta tagaccttcg cttcgttctc
    37261 cgtcaggttg atcgcagaac tccgtgcggt cgggatgatg gcgtggtgcg catcgacttt
    37321 tttgtcatcc caacagcggt tgcgtatatc tggatcgacc actggctgcg gcaacagatc
    37381 tggtgcatga acgctgatag cattcatcac cgcgtggcgt ccggcaaaat gttcttctgg
    37441 caaatagcgg caatcggaac gcggataagt gattagcttg tgcgtttcgt acagcttctg
    37501 gcagatatca agcacgttct gcgcgctcag accaaaacgt ttagccgctt caatctgcaa
    37561 cgccgaaagc gaaaaaggca gcggcgcgga ttctgattcc cgtttatcgt tatagctggt
    37621 gacaatagct ggttgaccac taatgcggtt aaccacatgc tccgccagtg gacgatgtaa
    37681 taagcgccct tcttcatcct ggtacggttc acacgcttcg ctcggttgcc agatagcggt
    37741 aaaccgctca tcggcaggtg tcacgatatg tgctttgact tcaaagaagt ctttcgccac
    37801 gaagttttca atctcttcgt cgcggcgcac caccagtccg agtacgggcg tctgcacgcg
    37861 tcccacggaa agcacgccct gataaccggc attgcgaccg agaatggtat acgcacgggt
    37921 catattaatg ccgtacagcc agtcagctcg cgcacgcgcc agtgcagaaa cgcacagcgg
    37981 tacaaactcg ctattggagc gaagacgatc gatcgcccgc tcaaccgcct gcgggttcag
    38041 gtcgtttatc aaacaacgct gtacctgctg gcgcttttcc ggtgccagtt gcaggtagtc
    38101 cagcacttca tccaccagca gttgcccttc gcgatccggg tctccggcgt gaacgatttc
    38161 gctggcttca tgcaggaagc gtttgatgac attaagttgt ttagtcacgg agggtcgggg
    38221 ctgtaattgc cacttttccg ggacaatcgg caaatccgca agattccagc gcgcatagcg
    38281 gctgtcgtag gcgtctggct gcgcctgctc aagcaggtga ccgatacacc aggtcaccac
    38341 ctgaccatta ccgcactcga taaagccatc gcctttccgg tgcggtttag gcaggacatc
    38401 agcaatggcg cgcgccagac tcggtttttc ggcaataaac aaccgcattg aattaacgga
    38461 tctcaaccat ggcacgaccg ccgcgcgctg gcaccagttc gccaatcgcc gtcagttcaa
    38521 tgccaaactc ggcggctgta gctttgacct cattttctgc ttccggcatg accgccagca
    38581 gcaaaccgcc agaagtttgc ggatcgcaca gcagttcgcg cacttcacgc ggcatttcac
    38641 ccatcagatg accgtagctg gcaaagttac gttcggtgcc gccaggcact gcgcccaact
    38701 taatgtactc ttcaaccccc ggcaatttcg ggatcgcgtc atagtcgacg cgcgcctgca
    38761 cacctgcgcc ctgacacatt tcgcttaaat gccccagtaa accaaaaccc gtaacgtcgg
    38821 tcatcgcttt tacgccttcg atgttggcaa aggacgcgcc tgcgatgttc atccggcaca
    38881 tcacttccgt cgccagtccc tgatgttctg gtttcagcag tgattttttc tcagccgtgg
    38941 taagaacgcc aatccccaga ggtttcgtca ggaacagttt gcatccggct tgtgcggtac
    39001 tgtttttctt cacccgctcg gtaggtacga tccccgttac cgccagacca aaaatcggct
    39061 ctggcgcatc gatggagtga ccgccagcca gcgcaatccc cgcctgacgg catgcatggc
    39121 gtccaccttc ggtcacttcg cgggcaattt ccggggaaag tttgttaatc ggccagccga
    39181 ggatcgcaat cgccataatc ggtttgccgc ccattgcgaa gatatcgctg atggcgttag
    39241 tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat cggcataaag aagtcggtgg
    39301 tactgataac gctggtgcca ttgcccagat cgtataccgc cgcatcgtcg cgggtttcat
    39361 tacccacaag caaattcgga tcaacaaact tcgcctgctc actgtgcagg atggtttcca
    39421 acacttttgg ggaaatttta cagccgcaac cagcgccgtg gctgtattgg gtcaaacgaa
    39481 tcgagttctc gctcatggac atctcctgtc aatgcaatcc gggtatggta accctcattc
    39541 catgaagtga taagtgagag tgtctgaatt cctgcgcctt tgctcacaat ccagacagtt
    39601 tcgcgacaat tatcagaaat aagtcacaaa cggcgtcggg tccgggacgt ttatcgacgt
    39661 agatgctttc aactgcggcg taccgaggta gagaaaaccg acaattttat cctgctcacg
    39721 gcaaccgaat gcttcacgca ctaccggact ttcagttaat gcaccactgc gccagatgcc
    39781 gccaaacccc tgggcaactg ctgccatttg catcgccatg accgcgcatc ccgcagacat
    39841 ttcctgttcc cagcgcggga ctttatgatt ttcttcgcat ttcgccacca ccgttatgat
    39901 gagcggtgcg cggaacggcg cattacgcgc tttgtcgatg gctttgttat cactaccggc
    39961 agcaatcgcc ccctgctcca gtacggcgct gaaacgctcg cgcccttccc cttcaatcac
    40021 aaagaaatgc cacggttgca tggacttatg gtccggcgca cgcatagccg cacgcaggat
    40081 gttttgcagt tgttcacccg ttggcgcagg ttcagccagg cgggaggcgc tacggcgatt
    40141 gatcaatagt tcgagtgcat ccatttgatt aactcctgtc atgatattta ttcacaaaat
    40201 taacacgaga gtggattttg ttacagccca gtccgcaatt cctgctgaca agtgccggtt
    40261 gggtcattac gataaccaca tctattgcgc ctgaaacagg tgtgacctta agttgggaga
    40321 atacatgcga accctttggc gatttattgc cggatttttt aaatggacgt ggcgtctgct
    40381 gaatttcgtc cgtgaaatgg tacttaacct gttctttatt tttctcgtgc tggttggtgt
    40441 ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa acggccagtc gtggcgcact
    40501 gctgctggac atttctggtg tgatcgtcga taaacccgac agttctcagc ggtttagtaa
    40561 attaagccgc cagctgcttg gtgccagttc cgatcgtctg caggaaaact cactgtttga
    40621 tatcgtcaac accattcgcc aggcgaagga cgaccgcaat atcaccggta ttgtgatgga
    40681 tctgaaaaac ttcgcaggcg gcgaccagcc gtctatgcag tacatcggca aagctctgaa
    40741 agagtttcgt gatagcggga aaccggttta tgccgttggc gagaactaca gccaggggca
    40801 atattatctc gccagtttcg ccaataaaat ttggctgtct ccgcaaggcg tggttgatct
    40861 gcacggcttt gccaccaacg gtctgtacta caaatcatta ctggataagc tgaaagtttc
    40921 cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt gaaccgttta ttcgtgatga
    40981 tatgtcgccg gcagcccgcg aagctgacag ccgctggatt ggtgagctgt ggcaaaacta
    41041 tctgaatact gttgccgcta accggcagat ccctgctcag caggtattcc ctggcgcgca
    41101 agggttgctt gagggtttaa ccaaaaccgg tggcgatacc gcgaaatatg cgctggaaaa
    41161 caagctggtc gatgcactgg catcgagtgc cgaaatcgaa aaaactctga ccaaagagtt
    41221 cggctggagc aagactgata aaaattatcg cgccatcagt tattacgatt acgcattgaa
    41281 aacgccggca gataccggtg acagcatcgg agtcgtcttc gctaatggcg caattatgga
    41341 tggcgaggaa actcagggga atgttggcgg tgataccact gcggcacaaa tccgcgacgc
    41401 tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt aatagcccag gcggcagcgt
    41461 taccgcgtct gaagtgattc gcgctgaact ggcagcagcc cgggcagcgg gtaagcctgt
    41521 ggttgtatcg atgggcggca tggcggcatc tggtggttac tggatttcca caccagctaa
    41581 ttacattgtg gctaacccca gcaccctgac cggttctatc ggtatcttcg gcgtgatcac
    41641 caccgtagaa aatagtctgg attcgattgg tgttcatact gatggtgtct caacttcacc
    41701 gctggcggat gtttctatca ccagggcact gccgccggaa gcgcagcaga tgatgcagtt
    41761 aagcattgag aatggctata aacgctttat cacgctggtt gctgatgcgc gtcattcaac
    41821 gccagagcag attgataaaa tcgcccaggg ccatgtctgg accggtcagg atgcaaaagc
    41881 taacgggctg gtcgatagtc tcggggattt cgatgatgcg gtcgccaaag cagcagagct
    41941 ggcaaaagtg aaacagtggc atctggaata ctacgttgat gaaccgacct tcttcgacaa
    42001 agtgatggac aacatgtctg gttctgtccg ggcaatgttg ccagatgcgt tccaggccat
    42061 gttacctgca ccgctggcct cggtagcctc taccgttaaa agtgaaagcg acaagctggc
    42121 agcgtttaac gacccacaaa accgttatgc gttttgcctg acctgcgcca acgtgcgtta
    42181 agtcttgtac tgagtggccg acagatcgtc ggccacattt ttttacctcg acgaatcctc
    42241 ttcccgctgt ctcgccccat atttccttat ccgcatagta tcaggtgcgc tcccccttcc
    42301 tcacgtatat acttttgctc tttcgatatc attcatatca atatcatgca aaagaaatca
    42361 atttacgtcg cctacacggg cgggaccatc gggatgcagc gttccgagca gggttatatt
    42421 ccggtgtcag gtcatctaca gcgccaactg gcgctgatgc cggaattcca tcgcccggag
    42481 atgccagatt tcactattca tgaatatacg ccattgatgg attcttctga tatgacgcca
    42541 gaagactggc agcatattgc tgaagatatt aaagcgcact atgacgacta tgatggtttt
    42601 gtcattctgc acggcacaga cacgatggcg tataccgcct ctgcactgtc gttcatgctc
    42661 gagaatctcg gtaaaccggt cattgtgaca gggtcacaaa tcccgctggc tgagttacgc
    42721 tctgacggac aaattaatct gctgaatgcg ttgtacgttg cggcgaatta tcctatcaac
    42781 gaagtaactc tcttttttaa taatcgactg tatcgtggca acagaaccac caaagcccat
    42841 gccgatggtt ttgatgcgtt tgcctctcca aaccttcctc cgttactgga agcaggtatc
    42901 catattcgcc gtttgaatac gccacccgcc ccgcacggtg aaggggaatt gatcgttcat
    42961 ccaatcacgc cgcaaccaat tggcgtagtg acgatttatc cggggatttc tgctgacgtc
    43021 gtgcgcaatt ttctgcgcca acctgtgaaa gcattgattc tgcgctcata tggcgtgggt
    43081 aatgcgccac aaaacaaagc cttcctgcag gaattacaag aagccagcga tcgcggtatt
    43141 gtggtggtca acctgacaca atgtatgtcc ggtaaagtga acatgggtgg ttatgccacc
    43201 ggtaacgccc tcgcccatgc cggcgttatt ggcggtgcag atatgactgt agaagccacg
    43261 ctaaccaaac tgcattacct gcttagccag gaacttgata ctgaaaccat tcgcaaggcc
    43321 atgagccaaa acctgcgtgg cgaactgacg ccggatgatt aaggagactg caatgcaccc
    43381 tcgcgccctg ttactggtcg atttacaaaa tgatttctgt gctggtggcg cgctcgccgt
    43441 gccggaaggt gacagtacgg tggatgtcgc taaccgcctg attgactggt gccagtcgcg
    43501 cggtgaagcg gttatcgcca gtcaggactg gcacccggcg aatcacggca gttttgccag
    43561 tcagcacggt gtagagcctt atacgccagg ccaactcgac ggtttgccac aaaccttctg
    43621 gccagatcac tgtgtgcaga acagtgaagg cgcacaatta catccgttac tgaaccaaaa
    43681 agcgatcgca gcggtgttcc ataaaggcga aaatccttta gttgacagtt acagtgcctt
    43741 ttttgataac ggccgtcggc agaaaaccac gctcgatgac tggttacgcg atcatgaaat
    43801 cgatgaattg atcattatgg gtctggctac tgactattgc gtgaagttta ccgtgctgga
    43861 cgcgttacag ttaggttata aggttaatgt gattaccgat ggttgtcgtg gcgtgaatat
    43921 ccagccacag gacagtgcgc acgcgtttat ggagatgtca gcagctgggg caacgctata
    43981 tacgctggca gattgggaag agacgcagga gtaatattag ttggattcat ctctggccta
    44041 catttcctca atgacattgt aggccaggta atacttgtta gtgttgcatt taacctactc
    44101 gcagctaaaa cacgacatcc gaacaagtct atttgccctc ccttaataac aggcttaaat
    44161 actagacaac tcattcactt aaacatggca ataggctttg ctgagatacc aaaatcttct
    44221 tttaactgtt gtttgctctt catgaccatc tgaccatttg tgtcaattgt catatgctgt
    44281 gcttcggtat tattgcgggc ttgccacagc atcaccagtt gcaggcagtt ctctttttgt
    44341 tcctcggtca acgtaacgcc atcaggccat ttccccagtt caacggcggt cgacaaacgc
    44401 tggtatacct caggcgtcat gctgttgata atgtcatcaa gattcataat ttttccgctc
    44461 ctgtggaata agttgctgaa tcgttttttc aaccgttgat ttcttcgccg ttttcgccat
    44521 cagtgaagcg taaagaagca gagttaacac aataacgttc gcccgttggc tgcggcccgt
    44581 cagggaagac atggcccaga tgggcatcgc agttaccgca acgaatttct atgcgctgca
    44641 ttccatgtga caagtctttg atataacgaa tagattcttc actcaccggt tcgtagaaac
    44701 tgggccagcc acagccggaa tcatacttgg tttgggaatg aaacagcggg gcatcgcaga
    44761 tcaaacagtg atatacgccg tcacgcttgt tatgcagtaa acgacccgta aatggcggtt
    44821 ctgtcccatg attctgcgtc acgtagaact gcatctcgga caaatttttt ttcagttctt
    44881 ctgccgaagg tttattagcc atttgctcac atctcacttt aatcgtgctc acattacgtg
    44941 actgattcta acaaaacatt aacaccaact ggcaaaattt tgtcctaaac ttgatctcga
    45001 cgaaatggct gcacctaaat cgtgatgaaa atcacatttt tatcgtaatt gccctttaaa
    45061 attcggggcg ccgaccccat gtggtctcaa gcccaaagga agagtgaggc gagtcagtcg
    45121 cgtaatgctt aggcacagga ttgatttgtc gcaatgattg acacgattcc gcttgacgct
    45181 gcgtaaggtt tttgtaattt tacaggcaac cttttattca ctaacaaata gctggtggaa
    45241 tatatgacta tcaaagtagg tatcaacggt tttggccgta tcggtcgcat tgttttccgt
    45301 gctgctcaga aacgttctga catcgagatc gttgcaatca acgacctgtt agacgctgat
    45361 tacatggcat acatgctgaa atatgactcc actcacggcc gtttcgacgg taccgttgaa
    45421 gtgaaagacg gtcatctgat cgttaacggt aaaaaaatcc gtgttaccgc tgaacgtgat
    45481 ccggctaacc tgaaatggga cgaagttggt gttgacgttg tcgctgaagc aactggtctg
    45541 ttcctgactg acgaaactgc tcgtaaacac atcaccgctg gcgcgaagaa agtggttatg
    45601 actggtccgt ctaaagacaa cactccgatg ttcgttaaag gcgctaactt cgacaaatat
    45661 gctggccagg acatcgtttc caacgcttcc tgcaccacca actgcctggc tccgctggct
    45721 aaagttatca acgataactt cggcatcatc gaaggtctga tgaccaccgt tcacgctact
    45781 accgctactc agaaaaccgt tgatggcccg tctcacaaag actggcgcgg cggccgcggt
    45841 gcttcccaga acatcatccc gtcctctacc ggtgctgcta aagctgtagg taaagtactg
    45901 ccagaactga atggcaaact gactggtatg gcgttccgcg ttccgacccc gaacgtatcc
    45961 gtagttgacc tgaccgttcg tctggaaaaa gctgcaactt acgagcagat caaagctgcc
    46021 gttaaagctg ctgctgaagg cgaaatgaaa ggcgttctgg gctacaccga agatgacgta
    46081 gtatctaccg atttcaacgg cgaagtttgc acttccgtgt tcgatgctaa agctggtatc
    46141 gctctgaacg acaacttcgt gaaactggta tcctggtacg acaacgaaac cggttactcc
    46201 aacaaagttc tggacctgat cgctcacatc tccaaataag ttgagatgac actgtgatct
    46261 aaaaagagcg acttcggtcg ctcttttttt tacctgataa aatgaagtta aaggactgcg
    46321 tcatgattaa gaaaattttt gcccttccgg tcatcgaaca aatctcccct gtcctctccc
    46381 gtcgtaaact ggatgaactg gacctcattg tggtcgatca tccccaggta aaagcctctt
    46441 ttgcattaca gggcgcacac cttctctcat ggaaacctgc gggtgaagaa gaagttctgt
    46501 ggttgagcaa taacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc gtaccggttt
    46561 gctggccgtg gtttggtccg gctgcacaac aaggtccggc tgcacaacaa ggtctgcctg
    46621 cgcacggttt tgcccgcaac ctgccgtgga cgctgaaatc acatcgtgaa gatgctgatg
    46681 gcgtagcgct gacttttgaa ttgacacaaa gcgaagagac gaaaaaattc tggccgcacg
    46741 actttacgct attagcgcat ttccgcgtgg gtaaagcctg tgaaatcgat cttgaagcac
    46801 acggcgaatt tgaaaccacc tctgccctgc atacctactt taacgtgggt gatatcacta
    46861 aggtaagcgt cagtggactg ggcgatcgct tcattgataa agtgaatgac gcgaaagaag
    46921 atgtactgac cgacggtatt cagaccttcc ctgaccgtac cgatcgcgtg tatctgaatc
    46981 cgcaagattg cagcgtgatt aatgacgaag cgctgaatcg tattatcgcc gtaggccacc
    47041 agcatcatct gaacgtcgtt ggctggaacc caggaccggc gctttcagtt agcatgggcg
    47101 atatgccgga cgatggctac aaaacctttg tttgtgtaga aacggcttac gcttcagaaa
    47161 cgcaaaaagt gaccaaagaa aaacctgcac atctggcgca atccattcgc gttgcgaaac
    47221 gttaatttgc gttaatgttg tgtgccgggt gcgttgcatc cggcacgtat caacatcaca
    47281 ccatatccag cgcagttttt ccttttggtg ccggatatgc cttatccagc atagctaatt
    47341 ccgctgacga aagttcaacc tcaagcacag ccgcattttg ttggacatgg gcaatcgtgg
    47401 ccgcttttgg aatcgccatc acaccctgat gactgatcac ccacgccaac aatacttgtg
    47461 ccgcgctgat attgtgagca tgtgcaattt cgttgactac tgcgtttttt agcagcccat
    47521 tgcgcaaccg cccggcctga gctaacggac tgtaagccat caccggtatc tgctgttgct
    47581 ggcaccaggg gagtagatcg tactcaatcc ctcgtgaacc gagatggtaa agcacctgat
    47641 tagtggcaca ctgatttccc cccggcagct gccagagttc ctgcatgtcg gcataatcaa
    47701 ggttagaaac gccccagcgg cggatttttc cctgggcgat caatttttcc atcgctgcga
    47761 cagtctcttc aaaagcgaaa ctgccagacc agtgtaataa gtagagatcg agataatcag
    47821 tattgagacg gcgtaaactg gcttcgcatg catttatcgc tttttgcccg ccagcattcc
    47881 acggatagac tttagagacg agaaagacgt tatcacgcag accggttaat gcttccccaa
    47941 ccaccttttc agcaccgcct tcggcataca tttcggcggt atcaatgagg gttaaaccga
    48001 gttcaatgcc cgcgcgtagt gcagcaactt ctgttttgcg ctgactggca tcttcgccca
    48061 tataccatgt tccctgccct atggctggca gtgagacatc gccactaaat tgaatcattt
    48121 tttgttgcat tgtttcctcc caggtattgc accaccgtaa tgcaaaacag ggcatgacgc
    48181 cctgttttta tgcacaaaat gccctggaaa gatgcattat cagaatttgt aggtgatccc
    48241 ggtagaaatc aggccagtcc aggatttatc caccattggg ctgtcagtaa cttcatcaga
    48301 cagacgggtg tagcgcgcgg taccgtaaac actccagtcg ccgaggaagt tgtagctggc
    48361 gctcagctcc aggtaagggc tccagctgtc gttcgggtta tagccacgca gaccgctgcg
    48421 agcggactct ttgcgcgata cgccataata gtattcgttc tggttttcgc tgttccactg
    48481 cacaccaata cccggagtca cggtcaggcc accgttggtg taacgataca accaggccat
    48541 atcccagacg atgccgttgc tgttatccag ggtatcgcca gccagggtgg tacgcaggta
    48601 accgtactgg gtaaagtgag cataagacag accagccatc atggtgctct tacggtcatc
    48661 caggtgacgc atttggtgat cgccactgtc tttagctttg aagtaaagcg gcgaccagta
    48721 agcggtaatt gaaagtttgt ccgttgcgtc attccacagg tagtaaccac cacctaagcc
    48781 acggaaccag aagttatcgc cgtcatagtt gattaccggt actgggtaaa catcggtatc
    48841 gtaatcttta tatgggtgtt caacgacacc tacgcctgcg cccagggaaa atttaccttc
    48901 agcgtgcgct acgcctgcag acgttgcgat aagcactcca agtgccagaa gtttgagttt
    48961 ggtcacaatt aatcattcct taaacaaatg tttagcgggc gacaaagttt acccgtcaat
    49021 acatcgaaac ccaacctttt tacgttttca ttttttatag taactgttta attttcctga
    49081 cgcagatgac accactctta cagccaaatg aatttagcgt taccggcgac cctggtattt
    49141 acattaatta tgcaaaattt atggatgagt tgttgatatg ccattgaaat taagaaagcc
    49201 gtgcaggcaa gttttccatt tgccatctac gcttaatttt gaaggtgtat caccgggcac
    49261 gttgttctca tcgtcgataa aatggcatga gagttgctgt gttttagcaa gagacgtcgt
    49321 tcagtttact tcttccggga gcctctacta ttcatatgaa cggctcttaa cctgtgctaa
    49381 aaaacgaaag gacggcatac catgaatata ttcgatcact atcgccagcg atatgaagct
    49441 gccaaggacg aagagttcac actgcaggag tttcttacca cttgtcggca agatcgcagt
    49501 gcttatgcca acgcggctga gcggctattg atggctatcg gtgagcctgt catggtcgat
    49561 acagcccagg aacccagact ttctcgactc ttttctaacc gggtcatagc acgttatccg
    49621 gcatttgaag agttttacgg catggaagac gcgattgaac agattgtctc ttatctgaaa
    49681 cacgcggctc aggggctgga agagaagaaa caaatcctgt atctgcttgg gcctgtgggt
    49741 gggggtaaat catcgcttgc tgagcgactg aaatcattaa tgcagctcgt accgatttat
    49801 gtattgagcg ccaacggtga gcgtagcccg gtcaacgatc atccgttctg tcttttcaat
    49861 ccgcaggaag atgcgcagat tctggaaaaa gagtatggca ttcctcgccg ttatctcggc
    49921 accatcatgt cgccgtgggc ggcaaaacgc ctgcatgaat ttggtggcga tatcactaag
    49981 ttccgggtag tgaaggtctg gccgtcaatt ctgcaacaaa ttgctatcgc caaaacggaa
    50041 cccggtgatg agaacaacca ggacatctcc gcgctggttg ggaaagtcga tattcgtaaa
    50101 ctcgaacact acgcgcagaa tgacccggac gcctacggct attccggtgc actgtgccgc
    50161 gccaaccagg ggatcatgga attcgttgag atgtttaaag caccgattaa agtgctgcat
    50221 cccttgctaa ccgccactca ggaaggtaac tacaacggga cggaaggtat ctccgccctg
    50281 ccgttcaacg ggattattct cgcccactcg aacgaatccg aatgggtcac tttccgtaat
    50341 aacaaaaaca acgaagcctt cctcgatcgt gtttacatcg tgaaggtgcc gtattgcttg
    50401 cgcatttccg aagagatcaa aatctacgag aaattgctta atcacagtga attgactcac
    50461 gccccctgcg cccctggcac gctggaaacg ctgtcacgtt tttccattct ttcgcgcctt
    50521 aaagagccag aaaactccag catttattca aagatgcggg tttatgatgg cgaaagtctg
    50581 aaagacacag atcccaaagc caagtcgtat caggaatatc gtgactacgc cggtgtcgat
    50641 gaagggatga acggtctgtc gacgcgtttt gcgtttaaga tcctctcccg cgtgttcaac
    50701 ttcgatcatg tagaagtggc ggcaaacccg gttcatctgt tctacgtcct ggaacagcag
    50761 atcgagcgcg agcagttccc gcaagaacag gcagaacgct atctggagtt cctgaaaggt
    50821 tatctgatcc cgaaatatgc cgagtttatc ggcaaagaga tccagacggc ctaccttgaa
    50881 tcctattccg aatatgggca aaacattttc gaccgttatg ttacctacgc ggatttctgg
    50941 attcaggatc aggagtatcg cgatcctgat accgggcagt tgtttgaccg cgagtctctt
    51001 aacgccgagc tggagaaaat cgagaaaccg gcggggatca gtaatccaaa agatttccgc
    51061 aacgagattg ttaacttcgt actgcgcgcc agagcgaata acagcggacg caatccgaac
    51121 tggaccagct atgaaaaact gcgcacggtt attgagaaga aaatgttctc caataccgag
    51181 gagctgttgc cggttatttc gtttaacgcc aaaacgtcaa ccgacgagca aaagaaacat
    51241 gacgactttg tcgaccgtat gatggaaaaa ggctacaccc gtaaacaggt gcgtttactg
    51301 tgcgaatggt atttgcgcgt acgtaaatcg tcttaacaac cctggcccgg tacacatgct
    51361 accgggccta caacgacagc gaaccgtggg ccagagaagc ggcaacacag gcgtagcata
    51421 aagttggcaa atgtagtacg gggggcatat gacctggttt attgaccggc gtctgaacgg
    51481 caaaaacaaa agcatggtga atcgccagcg ttttttacgc cgttataaag cgcaaattaa
    51541 acagtcgatc tccgaggcca ttaataagcg ttcggtgact gacgtcgaca gcggcgagtc
    51601 cgtatccatt cccacggaag atattagcga accgatgttt catcaggggc gtggcggtct
    51661 gcgccaccgc gtgcatccgg gcaatgacca tttcgtccag aacgaccgaa ttgaacgtcc
    51721 ccagggtggc ggcggaggtt ccggcagcgg tcagggccag gccagccagg atggtgaagg
    51781 tcaggatgaa ttcgtctttc agatttcgaa agatgagtat cttgatctgc tctttgaaga
    51841 tttggcttta ccgaatctga aacaaaacca acaacgccag ctgaccgaat ataaaacgca
    51901 tcgggcgggt tataccgcta acggcgttcc ggccaatatc agcgttgtgc gttcattgca
    51961 gaactcactg gcgcggcgca cagccatgac ggcgggcaag cggcgggaac ttcatgcgct
    52021 ggaagagaat ttggccatca tcagcaacag tgaacctgcg caactgctgg aagaggaacg
    52081 tctgcgcaaa gaaattgcag aattacgtgc caaaattgaa cgcgtccctt tcattgacac
    52141 cttcgattta cgttacaaga actacgaaaa acggcccgat ccctccagcc aggcagtgat
    52201 gttttgcctg atggacgttt ccggttcaat ggatcagtcc actaaagata tggctaaacg
    52261 tttttatatt ctgctgtatc tgttcctcag cagaacatat aagaacgtgg aagtcgtata
    52321 catccgccat catacccagg cgaaagaagt cgatgaacat gagtttttct actcgcagga
    52381 aacaggcggc accattgttt ccagcgccct gaaactgatg gatgaggtag tgaaagagcg
    52441 ttataacccg gcacagtgga atatttacgt agcccaagca tcagacggcg ataactgggc
    52501 cgatgactct ccgctttgcc atgaaattct ggcgaaaaaa atattacccg ttgttcgtta
    52561 ttacagctat atcgaaatta cacgtcgcgc gcatcagaca ctgtggcgag aatatgagca
    52621 tctgcaatct actttcgaca actttgcgat gcagcatatc cgcgaccagg atgatattta
    52681 tccggtgttc cgtgaactgt ttcataaaca aaatgcaaca gctaaagact aaaactgtca
    52741 gccgggtcat tatcgcctgg ctgattttta gttcactgta aattatctca tttattacat
    52801 acattagctt acaatcgctt taaatatgac agcataacct ttacacaatt tagttccaga
    52861 aaacaatcat tcggaaaaat gattcggtca acacgtattt ccctggggtt attctttaat
    52921 tattttttat cgttaacgaa aattgatctt ggtcaaaact atatatctct gccatcaata
    52981 aaatccagca ctcacattgc tctccttttt atggcttcta tgggtacaca aaaattaaaa
    53041 gctcaaggct tttttatttt cagcttattg ctgacgttaa ttttattttg cattactacc
    53101 ttatataacg aaaacacgaa tgtaaaactc atcccacaga tgaattacct gatggttgtt
    53161 gtggctttgt ttttccttaa cgccgtcatt ttccttttca tgttaatgaa gtatttcact
    53221 aacaaacaaa ttttaccaac actcatttta agccttgcat ttttaagtgg ccttatctat
    53281 ttagttgaaa ccattgtaat tatccataaa ccaatcaacg gcagtacact gatccagaca
    53341 aagtcgaatg atgtttctat tttctatatt ttccgccaac tcagtttcat ttgtttaacc
    53401 tcgctggcgc tcttttgtta tgggaaagac aacatccttg acaacaataa gaagaaaact
    53461 ggcattctgt tgctggcgct gattcctttt ttagtttttc cccttctggc acacaatctg
    53521 agcagttata acgctgacta ttctttgtat gtcgtcgatt actgtccgga caaccatact
    53581 gcgacctggg gaatcaacta tacaaaaata ttggtttgtt tgtgggcatt tttactgttc
    53641 tttattatca tgcgtacacg attagccagc gaactatggc cgttaatagc attattatgt
    53701 ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa ttacaccatc
    53761 tggtatatca gtcgcgggat tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt
    53821 tataacattt ttcaggaact gcaactctcc agcaaactgg cagttcatga tgtactgacc
    53881 aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc gcgacctgtt
    53941 gttaaggact tttgtgtcat gctggttgat attaatcagt tcaaacgcat caatgcccaa
    54001 tggggacatc gtgtgggtga taaagtgctg gtatcaattg tcgatattat ccagcaaagc
    54061 atccgccccg atgatatttt agcgcgactg gagggtgagg tgtttggctt gctatttacc
    54121 gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc
    54181 ctgaccggct ttagtaacag atatgatgtt cctgagcaaa tgaccatcag tattggcacg
    54241 gttttttcaa cgggtgacac acgtaatatc tcgcttgtca tgacggaagc agatcaagcc
    54301 ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa
    54361 aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac tattacccct
    54421 aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt gccaggccgc
    54481 aatcgaactt tatctggttt tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa
    54541 ttgcaccata gaatgctccg gcattttatc gccgcaagtg tcattgtgct gacatcttcc
    54601 ttccttattt ttgaacttgt cgccagcgac agagcaatga gtgcctatct gcgctatatc
    54661 gtgcagagag cagactcctc ctttctttat gataagtatc agaatcagag tattgccgcg
    54721 catgtgatgc gcgctctcgc tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc
    54781 atctgcgagg cttttgagtc tgccaataat acccatggct taaacctgac tgcccataaa
    54841 tacccggact tacgcggcac actacaaacc gcatccactg actgcgacac aattgtggaa
    54901 gctgcagcac tattacccgc ttttgatcag gcagtggaaa gcaaccgcca ccaggatgat
    54961 tacggttcag gtcttgggat ggccgaagag aaatttcact attatctcga tctcaatgac
    55021 cgctatgtct atttttatga accggttaat gttgagtact ttgcgatgaa taactggtct
    55081 ttcctgcagt caggaagtat tggcatcgat cgcaaagata ttgaaaaggt atttaccggg
    55141 cgtaccgttt tatcgagcat ttaccaggat cagcgtacta aacagaacgt gatgagtttg
    55201 ctgacgccgg tatatgtcgc agggcagcta aaagggattg tgctgctgga tattaacaaa
    55261 aacaatctgc ggaatatctt ttacactcat gaccgccctc tcctctggcg ttttctcaat
    55321 gtcacgctaa ccgataccga ttcggggcgc gacattatca tcaaccagag cgaagataat
    55381 ctgttccagt atgtcagtta cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc
    55441 attgatattc tttactttat cacgtcttcg tggaaaagcg ttctgttctg gattttgacg
    55501 gcgttaattt tgctgaatat ggtgcggatg cacttccgtt tataccaaaa cgtgtcgcga
    55561 gaaaatatta gtgatgcgat gactggactg tataatcgca aaattttaac ccctgaactg
    55621 gagcagcggt tgcagaagct ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac
    55681 atggacaagt taaagcaaat aaatgacacc ctcggtcatc aggaggggga tttagcgatt
    55741 acgttattag ctcaggcgat taaacaatcg attcgtaaaa gtgattatgc catccgactc
    55801 ggtggcgatg aattctgcat cattcttgtc gattcgacgc cgcaaattgc agcacaactg
    55861 cctgaacgta tcgaaaaacg tctgcaacat atcgcgccgc agaaagagat cggcttctct
    55921 tccggtattt acgcgatgaa agaaaacgat acgttacatg atgcgtataa agcttccgat
    55981 gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc ttctgtggtt
    56041 gtttgcttgt aatctcagga gcatgaaatg actgaaatgg ctaaaggaag cgtgacgcat
    56101 cagcggttaa tcgctttatt atcacaagaa ggtgctgact tccgcgtcgt tacccatgaa
    56161 gcggtaggta aatgtgaagc ggtgtcagaa attcgtggca ccgctttagg ccagggggct
    56221 aaggcactgg tatgtaaagt caaaggcaat ggtgtaaatc aacatgttct ggcgatcctc
    56281 gcggccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg attacgcgct
    56341 tcgttggcca gcccggcgga agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc
    56401 cctttcagct tccatccaaa actcaaactg gttgccgatc ccctactctt cgaacgattt
    56461 gatgaaatcg ccttcaatgc aggcatgctg gataaatccg ttattttgaa aaccgcggat
    56521 tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta actagcctgt
    56581 ccgttcgata agaagaacgg acaaaaccag cacaacagca atggcaaaaa acgatgacgt
    56641 gataatcagt gtctcgacga acatttgatc gttcatagta tgcccccggg agatagttgt
    56701 tttcacgtta ttgttagcgt gcgcaaatgg cagtttgatg acagttcgcc tgatttttta
    56761 tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcgtaaagt aagcaggctt
    56821 attattcttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt
    56881 agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc tggtgttaat
    56941 cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac aggggctaag
    57001 tatcgggata atcattctga cgattggcgt tatggctccc atcgccagcg ggacgctacc
    57061 gccctcaacc ctgattcact cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtcgg
    57121 cgttattgtc tcctggctgg gggggcgcgg cgtgacgtta atgggcagcc agccgcaact
    57181 ggtcgccggg ctgctggtgg gcactgtttt gggcgtggcg ctgtttcgcg gcgtaccggt
    57241 tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt agttaatccc
    57301 gcaatatatc ggcctggcgt ttgccccagg cctttcttaa acatagtaat aaaggccgtt
    57361 gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt cactaatccc
    57421 tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcac ccccgtctct
    57481 ttcactatca gtcgggcaag attgcgttca ctcatcgcaa aaaagcgagc ccattgcccc
    57541 aatgaccccc actcgacggg ctctttcgcc atcatctcca ccatcgcgcg gattttagga
    57601 tgagaagaaa cgggtaggtg cagctgttgt tgtggctgct gagggagttc atcaaacagg
    57661 acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct gtttgccagg
    57721 gttaaaatta attcccggca taagggcgat attttcaatg tgcaacaggt tgtcggcatt
    57781 gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt cacttgattg
    57841 ctatgttcca cccctcccgg tatccaaacc gcatattgtg gcggcaccat ccacaaagca
    57901 ttttccaccg tacaggtaat tgcaccatgt agtgccagta tcaactgtcc tttgcggtgc
    57961 tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct
    58021 tcatggtgtc ggtcgggttc atagccattg agattcaacc tgtgcatcat tttgtccgaa
    58081 tttagcgata atttgtcatt ttagcttgat tcaacataac aataaaaacg gtaaggtaca
    58141 gcctcgtttg taacaatgag aagcatatga cctgttcaac ttcattaagc ggcaaaaaca
    58201 ggattgtcct tatcgctggc attctgatga ttgccacaac attacgcgtc acctttaccg
    58261 gcgcagcacc gttactggat acgattcgtt ccgcttactc gctgacgacg gcgcaaaccg
    58321 gcttattgac caccctgcca ttattagcct ttgcgctaat ctcacctttg gctgccccgg
    58381 tggcgcgacg ttttggtatg gaacgtagcc tgtttgccgc gttacttttg atctgtgctg
    58441 gtatcgcaat tcgctctctc ccttcgcctt acttattatt tggcggtaca gcggtcattg
    58501 gcggtgggat tgcattaggc aatgtcttac tgccaggatt aattaaacgc gatttccctc
    58561 attccgtcgc cagacttacc ggcgcatatt ccctgacaat gggagctgca gcggcactgg
    58621 gatcgactat ggtcgtgccg ctggctttga acggttttgg ctggcaaggc gcgttgctca
    58681 tgctgatgtc ttttcctctg ctggctcttt ttttatggct gccacagtgg cgaagtcaac
    58741 aacatgcaaa tttgagtacc tcacgcgcct tacatactcg gggtatctgg cgttcgccgc
    58801 ttgcctggca ggtaacattg tttcttggga tcaactcact ggtctattac gtgattattg
    58861 gctggcttcc ggcgattctc atcagtcacg gctatagcga agcacaggcg ggttcactgc
    58921 atggtttgct gcaactagcc acagcagctc ccggtttgct gatcccactt ttcttacatc
    58981 atgtgaaaga tcagcgtggt attgcagcgt tcgttgcctt gatgtgcgca gtggccgcgg
    59041 ttgggctctg ctttatgcca gcacacgcga tcacctggac tctgcttttc ggttttggtt
    59101 ccggcgcaac aatgatactg gggttgacgt tcattggtct gcgggcaagt tctgcgcatc
    59161 aggcggcggc actctcgggg atggcacaat ccgtcgggta tttgctggca gcctgtgggc
    59221 cgccgctgat gggtaaaata cacgatgcta acggtgactg gtccgtacca ctgatgggtg
    59281 ttgcaatact ttcactactg atggcgattt tcggactttg cgccgggaga gacaaagaaa
    59341 ttcgctaata tccggtgcta tagtgacgtg gcaaatcatg cgtgaaaggg agaacaaata
    59401 cgatgaatat tcagtgcaaa cgcgtttatg atccggctga acagagcgat ggttatcgcg
    59461 tactggtcga ccgcctctgg ccacgcggta tcaaaaaaac cgatttagcc cttgatgaat
    59521 gggataaaga aatcacgccg tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg
    59581 attttgcaac ctttcgcgag caatatcttg cagaactggc gcaacacgag caagaaggaa
    59641 agcggctggc gaacatcgcc aaaaaacagc cgctgaccct gctctactca gcaaaaaaca
    59701 ccacgcagaa ccatgcgctg gtgctggcag actggctacg tagcttgtga ttttagtacg
    59761 ccatccggca gttatttttc accagccgga tggtcccgtc gccacaatgc ccattcatca
    59821 atcgtttcac cgcccggtaa tttgcaattg ttgctgaccc cttgcgctgt ctgcacagga
    59881 atgagcgtcc cgcccttctg ctggcaatag accgacgccg gatttgccat accaatctgc
    59941 ggcggtttag gtgcttctgg ctgagaaggg gttgaacaac cagccaggac cagcaagcaa
    60001 ggcagaacaa aactgataat tttcatttat tgatctcaca tatttatcca gacttagagt
    60061 atcgcggtat cgttttgttt tgcagcacta tttttattac attcactcaa atcatattac
    60121 gccttgtttc atctttgttg atgatgtttt atcatgcctg caaagattaa ataatcagca
    60181 tttacccgcc gtatcctgga gttgtttcgt gtcagatcag attatcgccc gcgtctcgca
    60241 atcccttgcc aaagaacagt cactggaaag cctggtccga cagcttctgg agatgctgga
    60301 aatggtcact gatatggaat caacctacct gaccaaagtg gatgtcgaag cgcgcctgca
    60361 gcatataatg tttgcccgta acagccagaa aatgcacatc ccggagaatt ttaccgtctc
    60421 gtgggattac tcgttatgca aacgcgccat tgatgaaaac tgctttttca gcgatgaagt
    60481 ccctgaccgt tggggcgact gtattgcggc acgcaatctt ggcatcacca catttctgag
    60541 cacgccaatt cacttaccgg atggatcatt ctatggcacg ctttgcgccg ccagcagtga
    60601 gaagcgccag tggagtgaac gcgcggaaca ggttttacag ttattcgccg gactgattgc
    60661 acaatatatt caaaaagagg cactggttga gcagctgcgc gaagccaatg ctgcgctgat
    60721 tgcgcaatcg tataccgact cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa
    60781 tctgacgacg ctgttttctc tcgcccgaca tcttaaccat aagataatga tcgcgtttat
    60841 cgatctggat aacttcaaat taatcaacga tcgttttggt cataatagtg gtgatttgtt
    60901 cctcattcag gttggcgagc gccttaatac gctccagcaa aatggcgaag ttattggtcg
    60961 tctcggcggt gatgagtttt tggttgtttc actgaacaac gagaatgcgg atatttcgtc
    61021 tctgcgagaa cgtattcaac agcaaatacg tggagaatat cacttaggtg acgttgattt
    61081 gtattatccc ggtgccagtc ttggcatagt agaagtcgat cctgaaacga ccgatgcaga
    61141 cagtgccctg catgctgccg atatcgcgat gtatcaggag aaaaaacaca aacagaaaac
    61201 accttttgtc gcgcatccag cgctacattc ctgaggcgta ttcacatcct tttgattggt
    61261 gataacatgc gaatcggtat tatttttccg gttgtaatct ttattacagc ggtcgtattt
    61321 ttagcatggt tttttattgg cggctatgct gccccgggag cataaagatg aaaaaaacaa
    61381 cgattattat gatgggtgtg gcgattattg tcgtactcgg cactgagctg ggatggtggt
    61441 aacgtcacat ctagaaaata gcaaaggctg cctgtgtgca gcctttgtgc aatttacgcg
    61501 ttaactttta atctttctgt agataaacag cacgacaatc gcgccaataa cggcaaccac
    61561 gaagctgcca aaattgaagc catcgacttt accaaagcca aacagcgtgc tgatccatcc
    61621 gccgactacg gcaccgacta tccccagcag gatagtcata aagaatccac caccatcttt
    61681 acctggcatg atccacttcg ccagaatacc ggcaataagc ccaaaaataa tccatgacag
    61741 aatgcccatt gtttcctcac ttatctgttt tgcattagcg ggttagtcgc tgataaaaag
    61801 catagcacaa catcgtgagg gcaagatttg tgacgagcat cacggaggat ttttttgcga
    61861 tggcgcagaa attgcgccat caacgatcag tgataattac caaccacaaa catcatgttc
    61921 gttttccgtg tcataagaac gcacggtatt caccagatct tttatcactt cagccgccac
    61981 ttctggcacc agcaaagtca tcggcgtctc tgtttcataa tcgacagaaa cgccattgct
    62041 gttattggtg acggtcacgg tatacgttgc tttgcccatg attcatttcc cgttatgaat
    62101 gactttccgt tgttgcgcac cttccatcag gacttcagga gcaacgaaga agtcaatgtt
    62161 gaaataagtg tcgtcagtca tggcttcaat gttgtgccac ttttctggag ggaacacagc
    62221 aaactgcccc gcttcgataa ggatcacttg atcaggctct gtactgtgtt catcagcgta
    62281 gccgagatat ttgaccgccc catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt
    62341 tcctttatca agatgacgtt cgaatattcc ggcaggcgca gtttgtttat tccagaaagg
    62401 cgttgagcgc gtatgaatat aattctgtgg gatttgcagc atccttttcc ctccttcggt
    62461 gaatgcgctg aaaacggttt atttcagccg tttcagggta cgcctgataa tttgcatttt
    62521 aaataccatt tattggttac tatttagcac cgtatcagag aagaatcagg gaggattaca
    62581 ggtgggaagc ccgcgacaac gcgggctgaa tgcatcagga ctggagcgtc gccagtcggg
    62641 cggcgaaacc cacaaacatc aaaccaatca gtgagttgcc cactttagcc agtttctttt
    62701 tggtacgtat gtactgcgtg acaaaagcac cagaaataat caggaagctc aaatagcaga
    62761 aactcaccag ttccagcgtc gtcgccagaa taaagaatga aattcccgta tgtggggcat
    62821 taacatcgat aaactgtacg aaaaacgaca catagaacaa aatggctttc ggattagtca
    62881 ggctcaaaat taacgcgcgt ttaaaaatgg caccgtattg gggctcatcg gatttggcct
    62941 cgttattttt acccttcagg gtcgcgtaga gaattttact ccccagatag agcaaataaa
    63001 acgcaccaag ataacgtaca atgttgaata atatcggggt ggtcttaatt aatgtcgcca
    63061 ctccagccca tgccagaaac atcaataccg catcgccaat aaatacaccg caggccgcaa
    63121 gataaccgcc tttcataccg ctactgacgc tatttttgag tacaaacagg gtatttggcc
    63181 ctggcaccaa cacaataaaa atggccccaa ccagataggt ccagtaattc agaaccccgt
    63241 attcagcgaa cacattaacc tctttaatta tctttcgatc atgcgcgatt aaaggtgaat
    63301 atgctaacca atctgtagcg gcttagaaag gagaaaatca ggttttaacc tgatatcaac
    63361 ccgataattg aatcattaat caggcatgct ccagtgaaaa attcgggtag tgctcggcaa
    63421 aatactggcg taaaaattct accgttatcc gtactttcgc tgacgtcgcc agccttgaaa
    63481 cataaacggc ccagacattc gctggctggt aatattccgg tagcacttgt actaaatgac
    63541 cactggcaat gttttcgcta acatcccacc aggagcgcag agcaatcccc tgcccatcga
    63601 gacaccactg atggacaatt tctccatgat tagacgacag tgggccggta actttaatcg
    63661 catggtggcc ttctttattg cgtaattgcc agacaccaaa aggatgatcg cgctctttaa
    63721 tgaccaggca cggcaatgca gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa
    63781 taaattccgg tgacgcgcag agaatgcgat aattggtcgc cagtttgcgg gcgattaaat
    63841 taggggcgat gtcatcgcca atgcgaatat cgagatcgac accttcattg accaaatcga
    63901 ccaaccggtc ttccacatca aaacgtaatt ccagttgcgg atacgccttc gccagcgctg
    63961 ataacgccgg ggccaccacc tgtcgtccaa aaccaaagct gctgataata cgcagcatcc
    64021 cctgtggcac ctgacgcacg tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa
    64081 tccgctgcgc ccattcataa atccgctctc cttcttcggt aatggtgacg cggcgggtgg
    64141 tgcggtgtaa caacaccacg tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga
    64201 acgccggaga aacgcccagt tcttccgcca cggcggcaaa accagcccgg cgagccacca
    64261 gcataaagac gcgcaaatcg ttcagcagcg gtaaattatt catgattcgt gttttatgtt
    64321 tcaccagtta cagggattaa ttccttttca gtcaattata ggatggtgat gttgtcaatt
    64381 ttgatggtca ggaagtgaga acccaatgat gaaaacgatg cgtattgctg cgatcccggg
    64441 agacgggatt ggcaaagaag tccttcctga agggattcgc gtgttacagg ctgccgctga
    64501 gcgctggggc ttcgccttga gttttgagca aatggagtgg gcgagctgcg agtattacag
    64561 ccatcacggt aaaatgatgc cggacgactg gcatgagcaa cttagccgtt tcgacgccat
    64621 ctattttggt gccgtcggct ggccggacac cgttccggac catatttcgt tgtggggttc
    64681 gctgctgaaa tttcgtcgtg aattcgacca gtacgtcaac ctgcgcccgg ttcgtctctt
    64741 tcctggcgtt ccctgcccgc tggcgggaaa acagcctggc gacatcgatt tttacgtggt
    64801 cagggaaaac accgaaggcg aatattcctc gctcggcggc agagtgaatg aaggtacaga
    64861 gcatgaagtc gtcattcagg aatcggtatt tactcgtcgt ggtgtcgatc gtattttgcg
    64921 ctatgccttt gaacttgcgc aaagccgccc acgtaagacg ctaacttctg ccactaaatc
    64981 gaacggttta gccatcagca tgccgtactg ggatgagcga gtggaagcaa tggctgagaa
    65041 ttacccggag atccgctggg acaagcagca tattgacatt ctctgcgcac gttttgtgat
    65101 gcaaccggaa cgcttcgatg tggtagtggc gtccaatttg tttggcgata tcctttccga
    65161 tcttggcccg gcctgcaccg gcaccattgg cattgcccca tccgccaacc tgaatccgga
    65221 acgcactttc ccgtcgctct tcgagcctgt ccacggttcc gcgccggata tctacgggaa
    65281 aaaaattgct aaccctatcg ccacaatttg ggccggtgca atgatgctcg attttctcgg
    65341 caatggagat gagcgtttcc agcaagcgca taacggtatt ctggccgcga ttgaagaagt
    65401 gattgatcac gggccgaaaa cacctgatat gaaaggcaat gccaccacgc cacaggttgc
    65461 cgacgcgatt tgcaaaatta ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca
    65521 ctgacgcggt ttttttattc gttctttgca gtaaataacc tgcgtcattt caccttttat
    65581 tgtttccgtt tcgtgtttta tggctttccg tattcttaat tgtttaattt atgtaacatg
    65641 caaataatca atttgttaat cacgacatac aactcattac cctgcaaata attacaactg
    65701 gcaaaagcca gagacgggct tttatgccca ggagatgact catgatgagc aatgttaaga
    65761 aaaaagatgt gccgctgata agcatcagcc tggtggccat tcttttcatc gcagctgcat
    65821 taagcctttt cccacaacaa tcggccgacg cggccaacgc aatatacact tttgttacgc
    65881 gtacgttagg ttccgccgta caggtattgg ttttgctggc aatgggactg gtgatttatt
    65941 tagccaccag taaatacggc aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc
    66001 tctcatggct gtttatgttt atttgtgccg gtttaggttc ttctacgctt tattgggggg
    66061 ttgctgaatg ggcctattat tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac
    66121 aggcactcga atttagcgtt ccctactcct tcttccactg gggcatcagc gcctgggcaa
    66181 cttatacgct ggcctcatta atcatggctt atcactttca tgtgcggaaa aataaagggc
    66241 tgagcctttc cggcattatt gctgctatta ccggcgttcg cccgcaaggc ccgtggggaa
    66301 aactggtcga tttgatgttc ctgatcgcca ctgtcggcgc actgaccatt tcccttgttg
    66361 tcaccgcagc aacctttacc cgtgggcttt ccgcgctgac cggtttaccc gataacttta
    66421 ccgtacaggc atttgtgatc ctgctttccg gcggcatttt ttgcctaagc tcgtggattg
    66481 gtatcaacaa cggtttgcaa cgtctaagca aaatggttgg ctggggcgcg ttcctgctgc
    66541 cattactggt gctgattgtc ggtccaaccg aatttattac caacagcatc atcaatgcca
    66601 tcggcctgac cacgcaaaac ttcctgcaaa tgagcttatt caccgatccg cttggcgatg
    66661 gttcatttac ccgcaactgg accgttttct actggctgtg gtggatctca tacacgcctg
    66721 gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg
    66781 gactgatcct cggcagcacc gtcggttgct ggttcttctt cggcgtaatg gaaagctatg
    66841 ccattcacca gtttatcaat ggcgtaatca acgtcccaca ggtgctggaa acactgggcg
    66901 gcgagacagc tgtgcagcaa gttctgatgt cgttgccagc cggtaaattg ttcctcgccg
    66961 catacctggg cgtgatgatt attttccttg cctcgcatat ggatgcggtg gcctacacca
    67021 tggcggcaac cagtacgcgt aatctccagg aaggtgacga tcctgaccgt gggctgcgtc
    67081 ttttctggtg cgtggtaatt accctgatcc cgctttcaat tcttttcact ggcgcgtcgc
    67141 tggaaacgat gaaaaccacc gtcgtactca cagcccttcc gttcctcgtc attttactgg
    67201 tgaaagtcgg cggatttatt cgctggctga aacaggatta cgccgacatt ccggctcatc
    67261 atgttgaaca ttatctcccg cagacaccgg ttgaagtcct ggaaaaaacg ccagtgctcc
    67321 ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc atcctaacga taaaggtatc
    67381 cctatgagca atctgagccc tgactttgta ctacccgaaa atttttgcgc taacccgcaa
    67441 gaggcgtgga ccattcctgc ccgtttttat accgatcaga acgcctttga acacgaaaaa
    67501 gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc
    67561 aatgattatg tgacgcgtga gatcattggc gaaagcattg tgctggtacg cggtcgtgat
    67621 aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggc
    67681 gaagggaaag cgaaaaatgt gattacctgc ccgtatcacg catgggcatt caaactcgat
    67741 ggcaacctgg cccatgcacg taactgcgaa aacgtcgcca atttcgatag cgacaaagcg
    67801 caactggttc cggtgcgtct ggaagaatat gccggattcg tcttcatcaa catggaccct
    67861 aacgccacca gcgtagaaga tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc
    67921 ccggaagtcc acgatctgaa actggcggcc cgctttacca cccgcacgcc tgccaactgg
    67981 aagaacattg tcgataacta tctcgagtgc tatcactgtg gtccggcgca tccaggtttc
    68041 tccgactccg tacaggttga tcgttactgg cacaccatgc acggtaactg gacgctgcaa
    68101 tacggtttcg ccaaaccgtc agaacagtct ttcaaattcg aagagggcac ggatgcggca
    68161 ttccacggtt tctggctgtg gccgtgcacg atgcttaacg tcacaccgat caaagggatg
    68221 atgacggtca tttatgaatt cccggtggat tctgaaacta ccctgcaaaa ctacgatatt
    68281 tacttcacca atgaagagtt aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat
    68341 gtgttccgtc cggaagattt acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt
    68401 ggctatcgtg gtcaggggcg catcatggcc gacagtagcg gtagcggaat ttccgaacat
    68461 ggtatcgccc atttccataa tctgctggcg caggtgttta aggactaatg acatcggcgg
    68521 cggtattttc cgccgctggg ctgatttttg atggagtaca gcaatgtcag actatcaaat
    68581 gtttgaagta caggtgagtc aggtggaacc gcttaccgaa caggtgaaac gcttcacgct
    68641 ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga ggaagtcaca tcatagtgca
    68701 gatgagcgat ggtgataacc agtacagcaa tgcgtattca ctactgagtt caccgcataa
    68761 cacctctagt tatcagattg ccgttcgact ggaggaaaac tcgcgcggcg gttcccgctt
    68821 tttgcatcag caggtaaaag tgggcgatcg gttaacgatt tcaacgccta ataacctgtt
    68881 tgcgctaatt ccctcggcca gaaagcatct gtttatcgcg ggcggtattg gtatcacccc
    68941 tttcctgtcg cacctggcag agttgcaata cagcgacgtc gactggcagc tacattactg
    69001 cacgcgaaat ccagaaagtt gcgcatttcg tgatgagcta gtccagcatc cacaggctga
    69061 gaaagttcac ttacatcact catcaaccgg aacacgactg gaattagcgc gattattggc
    69121 ggatatcgaa cctggcacac atgtttatac ctgtggcccc gaggcgctaa atgaagcggt
    69181 aagaagtgaa gctgcgcgtc tgaacatcgc cgccgatacg ctgcactttg agcaatttgc
    69241 tatcgaagac aaaaccggcg atgcatttac cctggtgctt gcccgttccg gaaaagagtt
    69301 tgtggtgccg gaagagatga ctattttgca ggttattgaa aataataaag cagcgaaagt
    69361 ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa acggcaatac tggaaggtga
    69421 agctgaccat cgggatcagt attttagcga tgaagagcgt gccagccagc aaagtatgtt
    69481 gatctgttgt tcgcgcgcga agggtaaacg cctggtgttg gatttgtagt ttgccgaagc
    69541 cggactgagc gcgcccagtc cggcttcgga agattttact gcggatattc ctgcaataaa
    69601 ttgtgtaatg cttccgccat cagctcacca cgccagccgg aaatcagctc cggcaaattg
    69661 ttctgcggtt tcagtttcca gtgccagttc agcagttggt tgatttgccg acgcgatgcc
    69721 agcaattcgg cgctgatctt gtgcgtttcg ctcacgtcag taatcagcga cttaatcgct
    69781 ttaaacgctt tacgataacc cggcatgtcc atcaggttaa gcatcggctg cggtaaggcc
    69841 tcttccggca atgcctgcgc tttttccacc agcgccagca gcgttttacc gtgaaagcgg
    69901 atttcgctac cggataagcc caggctgtcc agttcgccta aacttcccgg catataacgc
    69961 gctacggacc acaaatgctc ttcacgcacg acaaagttca ctgccagatc gcgctcccgc
    70021 gccttgcgca gtcgccagtc ggctaacagt tgcagacagg ccagttggcg tgtgcgtaac
    70081 tgccaggcat tggtgatatc acgccaggca tcttccggag caacgacttc ctgacgacgc
    70141 atttgcatca ggcggcattc atccagcgcc gcaggtagcc agccggaggc ctccgtttct
    70201 accataagct ttgcggtgat cggtaacaga taccagacat ccgccgctgc gtattcacac
    70261 tgacgttcgg tcagcggtct ggccagccag tcggtgcgcg attcactctt gtccagcgta
    70321 acgccggaat actcttccac catggaagcg aaaccccatg acatcgggcg tccgcagaag
    70381 gcagcaagga tttgcgtgtc aatcaagggt tgtggtaatt cgccaaagac attgaggaac
    70441 acttccagat cttcactgcc cgcatggaga aattttgtga tggacggatc gcgcaggatc
    70501 gctttcagcg gtgaccagtc agagatcccg aggggatcaa ttagcgccag atgctcacca
    70561 tcaaaaagtt gaattagacc cagttgcggg taataggtgc gcgtacgaac aaattcagta
    70621 tccagggcta tcgccggaaa ggcacggacg gcttcacaca aagaagccag cgcatcgtcc
    70681 gtggtaatca tttggtaatt caaattgtat tctctttagt gggcgtcaaa aaaaacgccg
    70741 gattaaccgg cgtctgacga ctgacttaac gctcaggctt tattgtccac tttgccgcgc
    70801 gcttcgtcac gtaattctcg tcgcaaaatt tttccgacgt tagatttcgg taactcatca
    70861 cgaaactcca ccagcttcgg tactttgtag cccgtgagct gacggcggca aaaagtcacc
    70921 agtgactctt cggtaagcga tggatctttt ttcacaacga agattttcac cgcttcacca
    70981 ctggagccgg aaggtacgcc aacagccgcg acttcctgta cgccaggatg ctgcatgacg
    71041 acatcttcaa tctcgttggg atagacgtta aaaccggaaa ccagaatcat gtctttttta
    71101 cgatcgacaa tgcgcaggaa tccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac
    71161 cagccatttt tgatgatttc atcggtagca tccggacgct gccagtaacc cagcatcacc
    71221 tgcggtcctt tgacacaaag ctcacccggt tgaccaggtg gtacttcact atcatcatca
    71281 tccaccagtt tggcttccgt cgacggcacc ggcaaaccga tgctaccact atgataatca
    71341 atatcatacg ggttaacgct caccagcggc gcacactcgg tcaggccata accttccagc
    71401 agatactgtc cagtcagttt aacccaacgt tctgccacca cttgctgaac aggcatccct
    71461 ccgcctgcgg aaagatgcag actggagaaa tccagctgct ggaactcttt attgttcagc
    71521 aacgcattga acaaggtgtt aacgccagtg atagcggtaa acggatattt cgctaactct
    71581 tttaccaacc ctggaatatc gcgcgggtta gtgataagca ggttctgccc ccccagttcg
    71641 ataaacagca ggcagttaat ggtcagtgca aaaatgtgat acagcggcag cgccgtcacc
    71701 accagctctt tgcccggatg caacagcgga ccataggtcg cgttaacctg ttccaggttc
    71761 gccagcatat tgcggtgagt cagcatcgcg cctttcgcca caccagtggt gccgccggtg
    71821 tattgcagga aagctaaatc ttccggcacc agttcgggtt tgacgtactg catccggtag
    71881 ccgttgtgca gggcgctacg aaatgaaatg gcatctggca gatggtattt cggcaccaga
    71941 cgcttgatgt atttaacaac gaaattgact accgtgcctt ttgccgtaga tagctgatcg
    72001 cccatacggg tcagaattac gtgctgaacg gcggttttat caaccacttt ttccagagtg
    72061 tgggcaaagt tagacacgat aacaatcgcc gatgcgccgc tatcgttaag ctgatgctca
    72121 agttcacgcg gggtatacaa cgggttaacg tttacgacaa tcatcccggc acgcaaaatg
    72181 ccaaacagcg ccaccggata ttgcaataaa ttaggcatca tcaacgcaac gcgatcgcct
    72241 ttcttcagtc ccaacccttg ttgcaaataa gcggcaaacg cgcgactgcg ttcttccagt
    72301 ttgcggaagg tcatcacctc ccccatattc acaaacgcgg gttgatcggc gtagcgcgcg
    72361 accgactgct caaacatatc taccagagat tgataacggt cagggttgat ctccgtcggg
    72421 acgtccgcgg gataacggtt aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg
    72481 ttcgtcgtca tcgcaacccc aaatgatata catgctgtta acataatatt aactcatcat
    72541 accagcttga taattaccca acgaaaaggt tgcgaagcgc gtcactattt atttttatct
    72601 ttaccgtaag aatgcagaaa cagcggacca gccgctgttt ctttttcttt aaaaacaagc
    72661 aattattcag ttacaacagt ttgtactctc gcggggccgg gattatacca gccccatcca
    72721 ccgtagccat agggccagcc acgaccgcca taaaaccatg gatcaatcgg ctgaggcggc
    72781 ataatcacct gctgggttaa atgccaacgt ttgtaacccg ttacctgcat caccataaat
    72841 ttatagggcg tgttgccgat tttgccgtca accgcaccgg tgattggccc aactaccgta
    72901 accagttgtc cacggaaatc taccgggtcc agaaaaccgt tcacatcagc ataaatgcga
    72961 ccgcgagaag gttctcccag cgtcggtctg gctccgctgt ccagtggtac ggtggcaatt
    73021 tccaggcggg ttttcccttg ctggttttgt accgcaacca ctttgccacc aaagcgtgcc
    73081 tcctgaccaa cgtacagctg cggcgcactc atcacccgaa ctaaatcttg ttgcggcgtg
    73141 ggactgctgc ctttaatggc gtccggcaca gtaacacaac cgctgagcat tagcgcaaac
    73201 gtacctgcca gtatgccttt gataacattc ttttgaaccg ccatgatgcg actccttttt
    73261 tctcagggca tactcttaag attcattctt tgcccggaag tttcttccat gcgacgttgt
    73321 tacgtaaata aaccggttcg gcatgttcca ccgccaccgt tttaccctcg gcaaacatct
    73381 gacacgcaat cggaagcata tcttctgcag cgggcagtaa cacttcgcca tcgcacaggg
    73441 tcaaaccgct ctctttaccg agatccggcc aggcttgcca gcctgtacct accgtcaccc
    73501 attccccgga aagctgttgc atccgttcat gaacgagttc gggtttgagt acggcttcgg
    73561 tttcttcacc gtgccagata cccttttcat cacgctgata ttcggcccag taaacttcgc
    73621 ccatccgcgc atcaatagca gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt
    73681 gcgccattgt catgagcgtg gaaacgccaa tcatcggcag atccgcgcca agcgccagcc
    73741 cctgcgcaat accaatacca atgcgcacgc cggtaaagct accggggccg cgcccgtaag
    73801 ccagagcgtt aatatcagtc agggaagttc cgctggtggt caggatatcc tgcaccatcg
    73861 gtaagattcg ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg acagtaccgt
    73921 cgttccacag ggcgacagag caggcctctg tcgcggtatc gatagccaga attcgcatgg
    73981 gtcttcgtgc ttagatcaat aaaaaggcgc gcatcatacc atactccgta acaaattacc
    74041 tggaggatgg tatcgcaagg aaacgaaccg cacgggcaat gtcacgggtg cgcggcgctg
    74101 gcggcagact ggcgagaaac gtcgcgccgt aagggcgcat caccagccga ttatcgcaaa
    74161 tcaccagcac gccgcgatcg tcggcgtcgc gaatcagtcg ccctactccc tgcttgagag
    74221 taatcaccgc atccggcagt tgtacttcat cgaacgggtc accaccacgc aaacggcaat
    74281 cttccatgcg cgcttttaat agtggatcat ccggtgaggt aaacggcaac ttgtcgataa
    74341 tcaccaatga caatgtatcg ccacgcacat ccaccccttc ccagaagctg ctggttgcca
    74401 caagaagcgc gttaccggcg ctgacaaatt gctgcaacag ttgccctttg ctggtttccc
    74461 cctgcaacaa aacgggaagc gtcatggtag cgcggaactg ctcagccaga tcgcgcatca
    74521 tggcgtgcga ggtacaaagc ataaaacaac gaccgttgtt agcttcgatg atcggtcgca
    74581 acattgccgc cagttgccgt gcggagcccg gttgattggt ttgcggcagg ttgcgcggca
    74641 cacagagtaa cgcctggcgg ctgtaatcaa acgggctggg taacagcaac gactctgcct
    74701 gttcaatgcc aagccgcgag gtaaaatgat gcagatcgtc gttcaccgac agcgttgctg
    74761 aggtaaagat ccagctaccg ggtttttgcg ccattaactc tttgaattta tccgccacgc
    74821 tgagcggcgt gagcgccaga gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc
    74881 ccggctgatt gatctctttt agccgtttca gccgcgtacg atacaacgtg gcgcgctcaa
    74941 atgccgcatc cagcaaggcg gaacgcccca gcgacagttt cgccacgtca taacaaagtt
    75001 ccagggtgtc atcgagcagt aaaaacgccc gttgaatttg cggattagct aacagttcgc
    75061 gcaggttgcc acgataacca ggctcaccga gttgcagacg aaaatcctgc gcgctctggg
    75121 caaggcggtc ggcgcacttt tgtaactgct gagtatcttt taattcggtg cgataggcga
    75181 tggtgatgtc ttttgccagg tcgagtagtt gtcgactgga gagtgactga ccaaaatact
    75241 ggctggcaat gtcaggcagc tggtgggctt cgtcgaagat catgacgtca gcttccggga
    75301 tcagttcgcc aaatccactc tctttcacca ccatatccgc cagaaagaga tgatggttta
    75361 ccaccaccac atcggcgtcc atcgcttttt tgcgtgcttt gaccacaaag caatctttat
    75421 acatcgggca gtcgctgcca aggcagttat cgttggtgct ggtgaccaac ggccacgcct
    75481 gtgaatcttc cgccacgctg acgcaggtgc tgatatcacc atcgactgtt tgattagacc
    75541 aggagcgcag caggatcaca tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg
    75601 cctgctgttc gagacgttcg aggcagaggt agtttgagcg ccctttcagc aacgccacgt
    75661 tgcccgtata tttcaacgcc tttgaaaccg ttggcaaatc gcggctgtag agctgatcct
    75721 gcaacgcttt tgagccggtc gagataatga ctttcttttt cgcccgcagc gcaggggcca
    75781 ggtaagcgta ggttttgccc gtaccggttc cggcttccac caccagcggc tggccttttt
    75841 ctatcgcctg ggtgacggct accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg
    75901 gtatcgcttt cgccagctga ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg
    75961 atttgaacag ggattatgtc aggatgaggg cgctttcgcc agttgaagtg gtgacggcga
    76021 cctcacattg tggcagtctt tgcagcacga aaatggaagt ataatgagga caaaatgatg
    76081 actatcgttc gtatcgatgc tgaagcccgc tggtctgatg tagtaatcca caacaacacg
    76141 ctctactaca ctggtgtacc ggaaaacctc gacgccgatg cctttgagca aaccgccaac
    76201 acgctggcgc agattgacgc cgtgctggaa aaacagggca gcaataaatc gagcattctg
    76261 gatgccacca ttttcctggc cgataaaaac gacttcgcgg cgatgaataa agcgtgggat
    76321 gcctgggttg tcgcgggtca tgcgccggtg cgctgcacgg tacaagcggg tttgatgaac
    76381 ccgaagtata aagttgaaat taagattgtg gctgcggtgt aagctttatc gaagcaaaat
    76441 aagtcagacg ataatttatc gacaatactg gtcggttttg cataaatcga ccagagatta
    76501 agata
//
LOCUS       DAZGTJ010000013        67719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000013 DAZGTJ010000000
VERSION     DAZGTJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.013"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            160..1857
                     /gene="ypdA"
                     /locus_tag="ACHK8E_001151"
     CDS             160..1857
                     /gene="ypdA"
                     /locus_tag="ACHK8E_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP0343451.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1872..2606
                     /gene="ypdB"
                     /locus_tag="ACHK8E_001152"
     CDS             1872..2606
                     /gene="ypdB"
                     /locus_tag="ACHK8E_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP0343452.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2619..3476
                     /gene="ypdC"
                     /locus_tag="ACHK8E_001153"
     CDS             2619..3476
                     /gene="ypdC"
                     /locus_tag="ACHK8E_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0343453.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3479..5974)
                     /gene="ptsP"
                     /locus_tag="ACHK8E_001154"
     CDS             complement(3479..5974)
                     /gene="ptsP"
                     /locus_tag="ACHK8E_001154"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0343454.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5999..7036)
                     /gene="ypdE"
                     /locus_tag="ACHK8E_001155"
     CDS             complement(5999..7036)
                     /gene="ypdE"
                     /locus_tag="ACHK8E_001155"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0343455.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7036..8121)
                     /gene="ypdF"
                     /locus_tag="ACHK8E_001156"
     CDS             complement(7036..8121)
                     /gene="ypdF"
                     /locus_tag="ACHK8E_001156"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0343456.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(8136..9383)
                     /gene="fryC"
                     /locus_tag="ACHK8E_001157"
     CDS             complement(8136..9383)
                     /gene="fryC"
                     /locus_tag="ACHK8E_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0343457.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9405..9731)
                     /gene="fryB"
                     /locus_tag="ACHK8E_001158"
     CDS             complement(9405..9731)
                     /gene="fryB"
                     /locus_tag="ACHK8E_001158"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0343458.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9950..10915)
                     /gene="glk"
                     /locus_tag="ACHK8E_001159"
     CDS             complement(9950..10915)
                     /gene="glk"
                     /locus_tag="ACHK8E_001159"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP0343459.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11119..12375
                     /gene="yfeO"
                     /locus_tag="ACHK8E_001160"
     CDS             11119..12375
                     /gene="yfeO"
                     /locus_tag="ACHK8E_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP0343460.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12490..12816
                     /gene="ypeC"
                     /locus_tag="ACHK8E_001161"
     CDS             12490..12816
                     /gene="ypeC"
                     /locus_tag="ACHK8E_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP0343461.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12957..14195)
                     /gene="mntH"
                     /locus_tag="ACHK8E_001162"
     CDS             complement(12957..14195)
                     /gene="mntH"
                     /locus_tag="ACHK8E_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP0343462.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14531..15733
                     /gene="nupC"
                     /locus_tag="ACHK8E_001163"
     CDS             14531..15733
                     /gene="nupC"
                     /locus_tag="ACHK8E_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP0343463.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15782..17971)
                     /gene="pdeA"
                     /locus_tag="ACHK8E_001164"
     CDS             complement(15782..17971)
                     /gene="pdeA"
                     /locus_tag="ACHK8E_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0343464.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18179..18254)
                     /locus_tag="ACHK8E_001165"
     tRNA            complement(18179..18254)
                     /locus_tag="ACHK8E_001165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18219..18221),aa:Ala,seq:ggc)
     gene            complement(18294..18369)
                     /locus_tag="ACHK8E_001166"
     tRNA            complement(18294..18369)
                     /locus_tag="ACHK8E_001166"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18334..18336),aa:Ala,seq:ggc)
     gene            18590..18949
                     /gene="yfeC"
                     /locus_tag="ACHK8E_001167"
     CDS             18590..18949
                     /gene="yfeC"
                     /locus_tag="ACHK8E_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0343465.1"
                     /translation="MFKERMTPDELARLTDYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18951..19343
                     /gene="yfeD"
                     /locus_tag="ACHK8E_001168"
     CDS             18951..19343
                     /gene="yfeD"
                     /locus_tag="ACHK8E_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0343466.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(19394..20809)
                     /gene="gltX"
                     /locus_tag="ACHK8E_001169"
     CDS             complement(19394..20809)
                     /gene="gltX"
                     /locus_tag="ACHK8E_001169"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP0343467.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            21068..21143
                     /locus_tag="ACHK8E_001170"
     tRNA            21068..21143
                     /locus_tag="ACHK8E_001170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21101..21103,aa:Val,seq:tac)
     gene            21188..21263
                     /locus_tag="ACHK8E_001171"
     tRNA            21188..21263
                     /locus_tag="ACHK8E_001171"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21221..21223,aa:Val,seq:tac)
     gene            21268..21343
                     /locus_tag="ACHK8E_001172"
     tRNA            21268..21343
                     /locus_tag="ACHK8E_001172"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21301..21303,aa:Lys,seq:ttt)
     gene            complement(21454..22338)
                     /gene="xapR"
                     /locus_tag="ACHK8E_001173"
     CDS             complement(21454..22338)
                     /gene="xapR"
                     /locus_tag="ACHK8E_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP0343468.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(22379..22537)
                     /locus_tag="ACHK8E_001174"
     CDS             complement(22379..22537)
                     /locus_tag="ACHK8E_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP0343469.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(22590..23846)
                     /gene="xapB"
                     /locus_tag="ACHK8E_001175"
     CDS             complement(22590..23846)
                     /gene="xapB"
                     /locus_tag="ACHK8E_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP0343470.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(23907..24740)
                     /gene="xapA"
                     /locus_tag="ACHK8E_001176"
     CDS             complement(23907..24740)
                     /gene="xapA"
                     /locus_tag="ACHK8E_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP0343471.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            24988..25752
                     /gene="yfeN"
                     /locus_tag="ACHK8E_001177"
     CDS             24988..25752
                     /gene="yfeN"
                     /locus_tag="ACHK8E_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP0343472.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(25790..26716)
                     /gene="yfeR"
                     /locus_tag="ACHK8E_001178"
     CDS             complement(25790..26716)
                     /gene="yfeR"
                     /locus_tag="ACHK8E_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0343473.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            26806..27804
                     /gene="yfeH"
                     /locus_tag="ACHK8E_001179"
     CDS             26806..27804
                     /gene="yfeH"
                     /locus_tag="ACHK8E_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP0343474.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(27801..28019)
                     /gene="ypeB"
                     /locus_tag="ACHK8E_001180"
     CDS             complement(27801..28019)
                     /gene="ypeB"
                     /locus_tag="ACHK8E_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP0343475.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(28021..30036)
                     /gene="ligA"
                     /locus_tag="ACHK8E_001181"
     CDS             complement(28021..30036)
                     /gene="ligA"
                     /locus_tag="ACHK8E_001181"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP0343476.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(30107..31105)
                     /gene="zipA"
                     /locus_tag="ACHK8E_001182"
     CDS             complement(30107..31105)
                     /gene="zipA"
                     /locus_tag="ACHK8E_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP0343477.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            31335..32096
                     /gene="cysZ"
                     /locus_tag="ACHK8E_001183"
     CDS             31335..32096
                     /gene="cysZ"
                     /locus_tag="ACHK8E_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP0343478.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            32281..33252
                     /gene="cysK"
                     /locus_tag="ACHK8E_001184"
     CDS             32281..33252
                     /gene="cysK"
                     /locus_tag="ACHK8E_001184"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP0343479.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            33636..33893
                     /gene="ptsH"
                     /locus_tag="ACHK8E_001185"
     CDS             33636..33893
                     /gene="ptsH"
                     /locus_tag="ACHK8E_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP0343480.1"
                     /translation="MFQQEVTITAPNGLHTRPAALFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            33938..35665
                     /gene="ptsI"
                     /locus_tag="ACHK8E_001186"
     CDS             33938..35665
                     /gene="ptsI"
                     /locus_tag="ACHK8E_001186"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP0343481.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            35706..36215
                     /gene="crr"
                     /locus_tag="ACHK8E_001187"
     CDS             35706..36215
                     /gene="crr"
                     /locus_tag="ACHK8E_001187"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP0343482.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(36257..37108)
                     /gene="pdxK"
                     /locus_tag="ACHK8E_001188"
                     /gene_synonym="thiJ"
     CDS             complement(36257..37108)
                     /gene="pdxK"
                     /locus_tag="ACHK8E_001188"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP0343483.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            37213..37581
                     /gene="yfeK"
                     /locus_tag="ACHK8E_001189"
     CDS             37213..37581
                     /gene="yfeK"
                     /locus_tag="ACHK8E_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP0343484.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(37584..38495)
                     /gene="cysM"
                     /locus_tag="ACHK8E_001190"
     CDS             complement(37584..38495)
                     /gene="cysM"
                     /locus_tag="ACHK8E_001190"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP0343485.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(38629..39726)
                     /gene="cysA"
                     /locus_tag="ACHK8E_001191"
     CDS             complement(38629..39726)
                     /gene="cysA"
                     /locus_tag="ACHK8E_001191"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP0343486.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(39716..40591)
                     /gene="cysW"
                     /locus_tag="ACHK8E_001192"
     CDS             complement(39716..40591)
                     /gene="cysW"
                     /locus_tag="ACHK8E_001192"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP0343487.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(40591..41424)
                     /gene="cysT"
                     /locus_tag="ACHK8E_001193"
     CDS             complement(40591..41424)
                     /gene="cysT"
                     /locus_tag="ACHK8E_001193"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP0343488.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(41424..42440)
                     /gene="cysP"
                     /locus_tag="ACHK8E_001194"
     CDS             complement(41424..42440)
                     /gene="cysP"
                     /locus_tag="ACHK8E_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP0343489.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(42598..43389)
                     /gene="ucpA"
                     /locus_tag="ACHK8E_001195"
     CDS             complement(42598..43389)
                     /gene="ucpA"
                     /locus_tag="ACHK8E_001195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP0343490.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(43518..44375)
                     /gene="murR"
                     /locus_tag="ACHK8E_001196"
     CDS             complement(43518..44375)
                     /gene="murR"
                     /locus_tag="ACHK8E_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP0343491.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            44539..45435
                     /gene="murQ"
                     /locus_tag="ACHK8E_001197"
     CDS             44539..45435
                     /gene="murQ"
                     /locus_tag="ACHK8E_001197"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP0343492.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            45439..46863
                     /gene="murP"
                     /locus_tag="ACHK8E_001198"
     CDS             45439..46863
                     /gene="murP"
                     /locus_tag="ACHK8E_001198"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP0343493.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            46868..48169
                     /gene="pbp4b"
                     /locus_tag="ACHK8E_001199"
     CDS             46868..48169
                     /gene="pbp4b"
                     /locus_tag="ACHK8E_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP0343494.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(48227..49126)
                     /gene="yfeX"
                     /locus_tag="ACHK8E_001200"
     CDS             complement(48227..49126)
                     /gene="yfeX"
                     /locus_tag="ACHK8E_001200"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP0343495.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(49222..49797)
                     /gene="yfeY"
                     /locus_tag="ACHK8E_001201"
     CDS             complement(49222..49797)
                     /gene="yfeY"
                     /locus_tag="ACHK8E_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP0343496.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(49858..50307)
                     /gene="yfeZ"
                     /locus_tag="ACHK8E_001202"
     CDS             complement(49858..50307)
                     /gene="yfeZ"
                     /locus_tag="ACHK8E_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFP0343497.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(50294..50719)
                     /gene="ypeA"
                     /locus_tag="ACHK8E_001203"
     CDS             complement(50294..50719)
                     /gene="ypeA"
                     /locus_tag="ACHK8E_001203"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP0343498.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            50933..51802
                     /gene="amiA"
                     /locus_tag="ACHK8E_001204"
     CDS             50933..51802
                     /gene="amiA"
                     /locus_tag="ACHK8E_001204"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP0343499.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            51806..52705
                     /gene="hemF"
                     /locus_tag="ACHK8E_001205"
     CDS             51806..52705
                     /gene="hemF"
                     /locus_tag="ACHK8E_001205"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP0343500.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(52639..53763)
                     /gene="eutR"
                     /locus_tag="ACHK8E_001206"
     CDS             complement(52639..53763)
                     /gene="eutR"
                     /locus_tag="ACHK8E_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP0343501.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(53809..54309)
                     /gene="eutK"
                     /locus_tag="ACHK8E_001207"
     CDS             complement(53809..54309)
                     /gene="eutK"
                     /locus_tag="ACHK8E_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP0343502.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(54322..54981)
                     /gene="eutL"
                     /locus_tag="ACHK8E_001208"
     CDS             complement(54322..54981)
                     /gene="eutL"
                     /locus_tag="ACHK8E_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP0343503.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(54991..55878)
                     /gene="eutC"
                     /locus_tag="ACHK8E_001209"
     CDS             complement(54991..55878)
                     /gene="eutC"
                     /locus_tag="ACHK8E_001209"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP0343504.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(55899..57260)
                     /gene="eutB"
                     /locus_tag="ACHK8E_001210"
     CDS             complement(55899..57260)
                     /gene="eutB"
                     /locus_tag="ACHK8E_001210"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP0343505.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(57272..58675)
                     /gene="eutA"
                     /locus_tag="ACHK8E_001211"
     CDS             complement(57272..58675)
                     /gene="eutA"
                     /locus_tag="ACHK8E_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP0343506.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(58672..59898)
                     /gene="eutH"
                     /locus_tag="ACHK8E_001212"
     CDS             complement(58672..59898)
                     /gene="eutH"
                     /locus_tag="ACHK8E_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP0343507.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(60115..61302)
                     /gene="eutG"
                     /locus_tag="ACHK8E_001213"
     CDS             complement(60115..61302)
                     /gene="eutG"
                     /locus_tag="ACHK8E_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP0343508.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(61292..62128)
                     /gene="eutJ"
                     /locus_tag="ACHK8E_001214"
     CDS             complement(61292..62128)
                     /gene="eutJ"
                     /locus_tag="ACHK8E_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP0343509.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(62139..63542)
                     /gene="eutE"
                     /locus_tag="ACHK8E_001215"
     CDS             complement(62139..63542)
                     /gene="eutE"
                     /locus_tag="ACHK8E_001215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP0343510.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(63554..63841)
                     /gene="eutN"
                     /locus_tag="ACHK8E_001216"
     CDS             complement(63554..63841)
                     /gene="eutN"
                     /locus_tag="ACHK8E_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP0343511.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(63948..64241)
                     /gene="eutM"
                     /locus_tag="ACHK8E_001217"
     CDS             complement(63948..64241)
                     /gene="eutM"
                     /locus_tag="ACHK8E_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP0343512.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(64280..65296)
                     /gene="pta"
                     /locus_tag="ACHK8E_001218"
     CDS             complement(64280..65296)
                     /gene="pta"
                     /locus_tag="ACHK8E_001218"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0343513.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(65293..66096)
                     /gene="eutT"
                     /locus_tag="ACHK8E_001219"
     CDS             complement(65293..66096)
                     /gene="eutT"
                     /locus_tag="ACHK8E_001219"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP0343514.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(66093..66794)
                     /gene="eutQ"
                     /locus_tag="ACHK8E_001220"
     CDS             complement(66093..66794)
                     /gene="eutQ"
                     /locus_tag="ACHK8E_001220"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP0343515.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(66769..67248)
                     /gene="eutP"
                     /locus_tag="ACHK8E_001221"
     CDS             complement(66769..67248)
                     /gene="eutP"
                     /locus_tag="ACHK8E_001221"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP0343516.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(67261..67596)
                     /gene="eutS"
                     /locus_tag="ACHK8E_001222"
     CDS             complement(67261..67596)
                     /gene="eutS"
                     /locus_tag="ACHK8E_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP0343517.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        16168 a        18669 c        17044 g        15838 t
ORIGIN      
        1 tttactaacc agaagtgcat tatcaagaga tgcattgcta atacaccatt tatctatcaa
       61 tcccctttta aaattgtggt ttttcctcaa aaacggcctg ctggtcatca gccttcaggt
      121 tacctatcat agaggtttaa tccttattca gagtcacccg tgcacgaaat attcaacatg
      181 ctgctggcgg tcttcgatcg ggcagcgtta atgcttatct gcctgttctt tctcatccgt
      241 atccgtctgt ttcgcgaact gttgcacaag tcggcgcact ccccaaaaga gttgctcgcc
      301 gttaccgcca ttttctcgct gttcgccctg ttcagcacct ggtccggcgt tcctgtagaa
      361 ggttcgctgg tgaacgtacg tattatcgcg gtgatgtcag gcgggattct gtttggcccg
      421 tgggtgggca tcattaccgg cgtgattgcg ggtattcacc gttatttaat tgatatcggt
      481 ggcgtgacgg cgatcccctg ctttatcacc agcattctgg cgggttgtat atcgggctgg
      541 atcaacctga aaatccccaa agcacagcgc tggcgcgtcg gtattctcgg cggcatgttg
      601 tgtgaaacgt tgaccatgat tctggtgatt gtctgggcac caactaccgc attggggatc
      661 gatatcgtct ctaaaatcgg gattccaatg atcctcggca gcgtctgtat cggctttatt
      721 gtgttgctgg tacaaagtgt tgagggcgaa aaagaggcca gcgccgcgcg gcaggccaag
      781 ctggcgctgg atatcgccaa caaaacgctg ccgctgtttc gccatgtcaa tagcgagtca
      841 ttacgcaagg tctgcgaaat tatccgcgat gacattcacg ctgacgcggt ggcaatcacc
      901 aataccgatc atgtgctggc ctacgttggc gtgggtgaac ataactatca gaatggcgat
      961 gacttcatta gcccgactac ccgccaggcg atgaattatg gaaaaatcat cattaaaaac
     1021 aatgatgaag cccaccgcac accagagatt cattccatgc tggtgatccc attgtgggag
     1081 aaaggcgttg tgaccggaac gctgaaaatt tactactgcc acgcgcatca gataacctcg
     1141 tcattacagg aaatggcggt cggtctgtca caaatcatct ccacgcaact ggaagtttcg
     1201 cgcgctgagc agctgcgtga aatggcaaat aaggcagagc ttcgcgccct gcaaagcaaa
     1261 attaatcccc atttcctgtt taacgctttg aacgctattt catcgtcaat ccgtctgaat
     1321 ccggataccg ctcgccagct gatctttaat ctgtcgcgtt atctgcgcta taacattgaa
     1381 ttaaaagacg atgagcaaat cgatatcaaa aaagagctgt atcaaattaa agactatatt
     1441 gccattgagc aggcccgctt tggcgacaag ctgacggtta tctatgatat tgatgaagag
     1501 gtgaattgct gcattcccag cctgctgatc cagccgttgg tggaaaacgc cattgtccac
     1561 ggtatccagc cttgcaaagg taaaggcgtt gtcaccatca gcgttgcaga gtgcggtaat
     1621 cgggtacgca ttgcggtgcg ggataccggg cacggcatcg atccaaaggt gattgagcgg
     1681 gtcgaagcga acgaaatgcc gggcaataaa attggcctgc tgaatgtcca tcatcgcgtg
     1741 aagttattgt atggcgaggg gctgcatatc cgccgcctgg aaccaggaac ggaaattgcg
     1801 ttttacattc ctaaccaacg cacccctgtc gcctcacagg ctacgttatt gctttgagcc
     1861 ggagtgatat tgtgaaagtc atcattgttg aagacgaatt cctggcacaa caggaactga
     1921 gctggctaat taaagagcac agccagatgg agattgtcgg cacctttgac gacggtctgg
     1981 acgtgttgaa gtttttgcag cataaccgcg tcgacgccat ttttctggat atcaatattc
     2041 cgtcgctgga tggtgtgtta ctggcacaaa acatcagcca gttcgcccat aaaccgttta
     2101 ttgtgtttat caccgcgtgg aaagaacatg cggtagaagc gtttgaactg gaggcgtttg
     2161 actacattct caaaccgtat caggagtcac gcattaccgg gatgctgcaa aaactggaag
     2221 cagcctggca gcaacagcag accagcagta cgcctgccgc gacggtaacg cgtgagaatg
     2281 acaccattaa tctggtgaaa gatgagcgaa tcatcgtcac gccaattaac gatatctatt
     2341 acgccgaagc gcacgagaaa atgacctttg tctatacgcg gcgtgaatcc tacgtaatgc
     2401 cgatgaacat taccgaattt tgcagcaaac tgccgccatc gcattttttc cgctgccatc
     2461 gctcattttg tgtcaatctg aacaaaatcc gcgagatcga accgtggttt aataacacct
     2521 atattctgcg gctgaaagat ctggattttg aagtgccggt cagccgcagc aaagttaaag
     2581 aattccgcca gttaatgcat ctttaatcgg gaactttcat gaaagcacca ggcttgcccg
     2641 ccgatcaaca atttttcgcc gatctgttca gcggcctggt gcttaacccg caactactgg
     2701 ggcgcgtctg gtttgccagc cagcctgcct cgttaccggt gggcagttta tgtattgatt
     2761 ttccccgtct ggatatcgtg ctgcgcggcg aatacggcaa tctgctggaa gcaaagcagc
     2821 aacgtatggt tgaaggagaa atgctgttta ttccggcgcg cgcggctaat ttaccgatca
     2881 acaacaaacc ggtgatgctg ttaagcctgg tgttcgcacc gacctggctt gggttatcgt
     2941 tttacgatag ccgcaccacg tcgttgttgc atccggttcg ccagatccaa cttcccagcc
     3001 tgcaacgcgg cgaaggtgaa gcaatgctta ctgccctcac ccatcttagc cgttcgccgc
     3061 tggagcagaa tatcattcag ccgctggtgt taagtttgct gcatctttgc cgtaacgtgg
     3121 tgaatatgcc gccgggcaat tcgcagccgc gcggcgattt tctctatcac agcatttgta
     3181 actgggttca ggataattat gcccagccgc tcacccgtga gagcgtggcg cagtttttta
     3241 atatcacgcc aaatcatctg tcaaaactgt ttgctcagca tggaacaatg cgttttatcg
     3301 agtatgttcg ctgggtacga atggcaaaag cgaggatgat tttgcaaaaa tatcatctgt
     3361 cgattcatga agtggcgcag cgttgcgggt ttccggatag cgactatttt tgtcgcgttt
     3421 tccggcgtca gtttggtctg acgccgggag agtacagcgc ccgttttcag ggctaacgtc
     3481 agaaggttaa ttctgtttcc agcagcgtca ggatgctttg tgcatcttgc gcggcaaaca
     3541 acgactggcg gaagttttta ttcaccagtt tacgcgccag ctgcgagaag actttcacat
     3601 gattcatccc ttcgttagcg cccagtgtca gcatgatcac cagttcgact tcgcccattt
     3661 ctgactgcca gtcaaccggt ttcaccagtc gggcaatgct gatactggaa tgacggatcc
     3721 actgagattt ggtgtgcggg atcgctacgc caaaaccaac gccggtggta acaatctctt
     3781 cccgctgcca gacatcctct tccagctcga acggatgttc agtgcgcccg ttaacgccga
     3841 ggttaccgca caggaactgg atcgcctgct ctttattgct aaaagcctga tcaacaaaga
     3901 tattctccag cgccagcaga gggcgaacgt cttcttccgg cgtaaacgcg gtgagtaacg
     3961 cttcaatctc ctgcgcgctg cggcattcac atgcctgacg cgccagttcc cgacacgcct
     4021 cgctatccag ttgacgaagc tggcttttca ccgccggaat gcgcgggcta ctcatactca
     4081 gctcgtccag gcccagccca agcagtagcg gcagataacg gctttcaccg cccagttcac
     4141 cgcaaatacc tacccattta ccccgctgat gcgcagtggt aacaatctgc tgcaacatgc
     4201 gcaggaacga tggcgtaatc gggttataca gcggcgatac gcgcgggtta ttacgatcga
     4261 ccgcatacag atactgggtc atatcgttgg agccgatact gaagaaatcg acctcatcgc
     4321 agaagtgatc gatgatgtag cacaccgacg gcacttccac catgataccc agcgtaatcg
     4381 tctctgcatg acgcaggcca tcgcgcttaa gctcaacgat cgctttttgg atctcgccct
     4441 tcacccataa gatctgatcg aggctgtgaa ccattgggat catcaactgg gcgttgccga
     4501 aactggcggc gcgcaaaatg gcccgcagtt gagtgcggaa caggccagca aattccgggt
     4561 aaattcgtac cgcacgatag ccgaggaacg ggttctcttc ctggggaatg ttgaggtaag
     4621 gaatgctttt atcgccgccg atgtccattg tgcggaagat aatcggcttg tcgcccgcgg
     4681 ccagtagcac ctgctggtag gcttcaaatt gctcctgctc gtccggcgca ctgtcgcgat
     4741 ccatatagag catttcggta cgaaacagcc cgacgccttc cgcgccgttg gcaaacgcgc
     4801 caggcgcttc cagcgcggtg ccaatattcg ccgcgatatc aatgcgcttg ttatcacggg
     4861 aataggccag ctgcgcggcg gcctgcgcct gctgctgctg acgtttatcc gccagcatct
     4921 gcgcaacctg ataataaccg ctcaccgcgt cattcgggtt aatcgccaac acgccgcact
     4981 gggcgtcaag caccgccggt tgcccggcat agcgcgcaat ggcatccagc ggcaggccgc
     5041 tcagtaccgg gatcgccgag gcgcgggcca gaatcagggt atgcgaggtg cgcccggttt
     5101 tctccagaat catgcccgca agatttttta aattgaggct caaaaactgg ctaggggtta
     5161 aatcctctgc caccagaatg gtcggttttt ccagcaccag attgttgcgc ggttttagtt
     5221 ccggccaggt gatatgcagt aactgttcgc tgatatcgcg aatgtcgcta acccgctcgc
     5281 gcagataatc actggcagaa gcagaaagtt tggcgcaaac ctgctccata ttgctgatga
     5341 tcgccgcccc cagcccctga tgctgttctg ccatcaggtg acggatattg cctgcaaatt
     5401 catcatcctg aatcagcgac aaatgggcgc tgaggatagt tttgctttcg ccgtcacgct
     5461 cacgcaattg ctggttcagt tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg
     5521 aatcttgcgc actggcgggg atcgcccggt aactgtcgag gctgtcgctc tgtaacaggg
     5581 tcagcgtacc cacgccgacg ccgcttgcca gcacattgcc gtacagtaaa tcaggattca
     5641 gacggcttaa tgaacgcggc agcggatgcg ccgtcagttc tgcctgcgtt ggctggacgc
     5701 tgtcgctgtc gataaagcgc acctggatgt actcttccag cacgcgccgc gcctgctctt
     5761 catcgctgcc gctaatgttc aggctgcaac cgtcattaaa tagggtgccg gtgccaatca
     5821 gcgccagcga gcttttggca tctgcctttg cgttctggcg atggttaata aaagtgattt
     5881 cgctttgcca ctggctgcac tgttctttaa gttcccacgc cggacgagca tgcagaccgt
     5941 ttggcaaagg acagagaaat tgaatcgtta acataaccgc tccgggttaa tcaggagatc
     6001 atctgaaatc cgtcagttga acaaccgtct cacgcgtaag acgttgaata agggcagata
     6061 aaagctgctg catctgcaaa atgtcacggc aatcggcaat cgatgcggcg caatgtccgt
     6121 ggcgggtggc aggccccatc accactgtgg gtacgccagt gccggttaag tgcaccgccc
     6181 caccgtccgt gccaccgttg ctgaacatat ccgcctgcaa cggcacgcca atttctgccg
     6241 ccacggtttc aatccacgcg gtaagttttg gcggcgcaat cagcgattta tcgcttaaca
     6301 ccagcatcgg cccgttacca atctgacgat ggttagccgc gccataatca aagtttttcg
     6361 cccagcaggc ggtatcaagc acaatggcga cgtccggcga caccgcgcgg gtggcagttt
     6421 gcccgccgcg taatcccacc tcttcgctgg aacttgccac cagccacacc tccgcaggca
     6481 gttcggcgtc gtgcagctca cgtagtaacg tcaccagcag atagcaaccg aggcgatcat
     6541 caaaggcttt ccccatcacc cgctggtgag ggagaacctg aaaagttgta tcaaaggtga
     6601 cgcgatcgcc tggacgaatt cccgcctgca tcacttcgtc atacgagcgc gcaccaatgt
     6661 ccacgcgtat ggtgctgacg tcattcccct gccggtcgcc gtcaagcagg cctggaattt
     6721 tgcactcttc acgggtagtg atacgcaccg actgaagctg gcgggcagcc atgcgtacat
     6781 tgccaaccgg caacacatcg atcgcccctt cgcgggagat gctgcgcacc ataaatccca
     6841 cttcgtccat atgcgcacag atcatcacct tcggacctgt cgactcattg agtcggatca
     6901 gcaccgatcc cagcccatca aatcgcactt ctttttgcag gcgatccgct tcttccagca
     6961 ggatctgccg tacttcctgt tccgaggagg cgatcgcatc tgcctcgctc aacgctttta
     7021 ataacgataa atccattatg cctctcccgt gagcaacact gttttcggca tggcgtagag
     7081 cacttctacg ccttgcgggg tgaccagcac aacatcttcg atgcgcacgc ccccttgccc
     7141 tggcaaataa atccccggct ccacggtcag taacatgcct ggctgtagcg tcgtggtgtc
     7201 ccgcggtgaa aaacgcggat cctcatggac ttcaataccg atagcatgac cggtgttatg
     7261 accgaaatag tcgccataac cggcttctgt aatcacccgg cgcgcggcgt cgtcaacctg
     7321 ctggcagcgt acgccggggc gaatcgcgga gattgctgcg agctgtgcct gcaggacaat
     7381 ctgatagacg ttaaacagcg ggtgggattc ggcacttacc ccttcgccat tcaccagcaa
     7441 ggtgcgcgtc atatcagagc agtagccctg atacagcgca ccgaaatcga gagtgacaaa
     7501 ctcgcccgct gcaacaatct tgtcgctggc tttgccgtgc ggcagcgccc cacgccagcc
     7561 gctggcgaca atggtatcga aagaggcttt ttctgcgccc tgctggcgca taaaccactc
     7621 cagttcagcg gctatctcgc gctcgctcat ccccgcctga ataaagcggc gaatatgctc
     7681 tgcaccgcga tcggcaatcc cacaggcgag acggattttc tccacctcct ctggcgtttt
     7741 gagttgccgc agcacatccg gcgtggcgct aaccagtttc gcattgagtt cagactgcca
     7801 gcggtgcgcg gtttcccaac tcacctgctg gccttcaaaa ccgagcgttt gcaactgctc
     7861 atcggcaaag atttgcctga cgatggtggc aagcgtgtgc atcgcgtcga gcaaatgcag
     7921 ctggtaacct tgcgtgcggg cttctacatc cgcgtaatag cgcgagtcca ccagaatgtg
     7981 cgcactttca cggcaaatca ccacatagcc tgatccggtg gagatcccca gatgcggctg
     8041 tttgttctgc cgtgaggaga gaagcaccgc atccagttgt tgcgccttaa gccagtcgcg
     8101 cagcgaagcg agtaatgtca tcaggattgt ccttcttaca ggctatcgat taacaatttg
     8161 cctttacgga acatcatcag acgcaggaac accaccatca gcgcagttat gactgccccc
     8221 agcgcgatcc ccgccatgta gacgccgagg ttagtgacca gcggccatgc ccagatagcg
     8281 gactccggga accattgcac tgcgcccagc cagacagcgg cggttgagcc gacaatcgcg
     8341 ccgaccatat aggacggaat ggcggtgatg gggctttcca gcgcaaacgg aatcgcaccc
     8401 tcactgatcc ccataaacgc caggaacatg gcggttttac cctgcggata aagctgagca
     8461 ctgaacagac gtttgccggt taaacggcgg tcgataatgg tcgccagacc cagaccaatc
     8521 ggcggaataa caatagcgat ggaacgcgcg gtaaccggta atacgtggtc agtggtaaag
     8581 ctgaaggcaa cgaaaccagc tgctttgttg atcgggccac caaggtcgat tgctgtcgcc
     8641 gcggcgatcc ccatcgcgta cattagcgca cccttctcac ctgcggcagt cagcacggta
     8701 cggataccgc cgttgatcca gccaccaaag ggggtgatga cgtagtacat cgccagcatg
     8761 acaaaaatgg cggaaagaat cggtagcaaa aatgtggttt tgaacgctaa caggaaatcc
     8821 ggtaactgga ttttctggtt catccacttc acaaggtaac cagcaacgat agaaataatc
     8881 agggcaccaa tgaaggtgga cggcaccggc gaagaggtcg cccattgcat cgtgctggga
     8941 tcgaagttca gcagttgcgt cggctgggta gacatcaacc caccgataaa accagccggg
     9001 aacgccagtt tgccaccgat agagttcgcc acaaatgcag caaacatcgg aatggcaaaa
     9061 ccaaacaaca cgccgccgaa ggactgcgac aaccaggcaa atttcagtaa ggagaggtcg
     9121 aaaccggaga atttcccgct attaagagca tccatgatgc cgatatcagc aggaatttta
     9181 agccagctat aagcaatcag ctggctgaaa gcgaggatca caccgcccat aattaaggtc
     9241 ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga gttcacccca gaagctggtt
     9301 ttagaggcct gcggattctt aactgccgct gccgcaccgg atgcgccagg cacaacggtt
     9361 gcactgcgtt ttttaatggc cataacaatt atccctgtcg aatattactg ttgttcagag
     9421 gcaatcatct cttcgatttc tttgatgatg cccgcagcgt ttttaattgc gtcctgcaaa
     9481 gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat cttccggggt aaccgccacg
     9541 gagtggatga tgatggtcgc ttcggcgata tcctgcgcgg tcaggcgatt ctggatgccg
     9601 tccgcgccct gagtttcaat tttcacttca tagcctgctt ccaccgccgc ttcttccagc
     9661 gcctgggctg ccataaaggt gtgagccaga cccatcgggc aggcacataa agcaatcagt
     9721 ttcttactca tcgttaattc ctcgttcagt gatagttgac gagtgaaatt atgaacgtgc
     9781 aaaacaaatc gccgttacca gacaaataat gcatttactg gagaattaat cccgctgtac
     9841 ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag gaaaattgaa ttaactgtat
     9901 gatttaaaag attatcggga gagttacctc ccgatataac aggaaggatt tagagaatgt
     9961 gacctaaggt ctggcgtaaa tgtgcaccgg aaccaagaag gcccggattg tcatggacga
    10021 tgagatacac cggaatatca tggacatatt ctttaaagcg ccctttatct tcaaatgcgg
    10081 cacggaaacc agaggctttg aagaactcaa ggaagcgcgg cacgatacca cccgcaataa
    10141 acacgccgcc aaatgtcccg agattgagcg ccagattgcc gccaaaacgg cccataatga
    10201 cgcaaaacag cgacaacgcg cggcggcaat cggtgcagct gtcagccagc gcgcgttcgg
    10261 taatatcttt tggcttgaga ttttctggca ggcggttgtc ggctttaaca attgcgcgat
    10321 acaaattcac cagcccaggg ccagaaagca cgcgctctgc cgaaacatga ccaatttctg
    10381 cacgcagtat ttcgaggata atgccctctt cttcactatt cggcgcaaaa tcaacgtgac
    10441 cgccttcgcc tggcaagctt acccaacgct tatcgacatg gaccagatgc gcaaccccta
    10501 gccccgttcc ggcaccgtaa accgcaatag gcttaccttc taccggttct gcgccaccaa
    10561 actgaatcag atgctctttt ttcagcatcg ggatcgccat cgatacagcg gtaaaatcgt
    10621 tgataatttc cagatggcta aaaccgagat tctttttcat ttcggcaatt gagaacgccc
    10681 aggtatggtt ggtcattgcc acccagtcac cggtaattgg gcaagcgatg gcaatacagc
    10741 cgtcttgcac ctcgatctta tgttcttcaa gataaacgcg aatgaccgct tcgaggctgg
    10801 ggtaatcaag ccctgaatag gtcttagcct gcgagatttc accactggca atatcacaca
    10861 gagcaagacg tgcgttggtg ccgcccacat caccgactaa tgcatacttt gtcattcttc
    10921 aactgctccg ctaaagtcaa aataattctt tctcacactg taaatacctg ggggcataac
    10981 aacaacgccg gaaaggcagg ctccctgtaa atatcgatct gggtcacaca attactttat
    11041 cgtttcagca ccaattgcag cgatgccttt ttgcaagctg ggcaaactaa gtatctgacc
    11101 ccgcataagg aatagaacat gctccatccg cgagccagaa ccatgttgtt attatcgctc
    11161 cccgccgtgg caattgggat tgcgtccagt cttattctga ttgtggtgat gaaaatcgcc
    11221 tcggtattac agaatttgct ctggcaacga ctgccgggaa ctctggggat agcccaggat
    11281 tcacctctct ggatcatcgg tgtattaacg ctaacgggta ttgcagtggg gttggttatc
    11341 cgtttcagcc agggtcatgc cggaccagac cccgcctgtg aaccgctgat cggcgcacca
    11401 gttccgccct ctgcgctacc aggacttatc gtagcattaa ttcttggtct tgctggtggc
    11461 gtcagcctgg ggccagaaca tccgatcatg accgtcaata tcgcccttgc ggttgccatt
    11521 ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga ctattttagc ctctgccgga
    11581 accatcggcg cgctgtttgg cacacctgtt gcggcggcgt tgatattttc gcaaacctta
    11641 aatggcagta gtgaagttcc gctatgggat cgtctttttg cgccgttaat ggcggcagca
    11701 gctggtgcac taactaccgg attatttttc catcctcatt tttcactgcc cattgcgcat
    11761 tacggacaga tgcaaatgac cgatattctc agtggtgcaa ttgtcgcggc gattgccatc
    11821 gcagcaggga tggtcgccgt atggtgctta ccacgattgc acgcgatgat gcatcaaatg
    11881 aaaaatccgg tgctcgtgct gggtattggc ggatttattc tcggtattct gggggttatg
    11941 ggtggaccag tttcgctgtt taaagggctg gatgagatgc agcagatggt ggcaaatcag
    12001 gctttcagca ccagcgatta ctttttgctg gcggtgatta aacttgccgc cctggtcgtt
    12061 gctgccgcca gtggctttcg cggtgggcga atcttcccgg cagtgtttgt cggcgtggca
    12121 ttagggttga tgctgcatga gcacgtcccc gccgttccag cggcaataac cgtttcctgc
    12181 gctattctcg gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct ttttatggcg
    12241 gcagtcgttg tacccaatac cacattgcta ccgctgctct gtatcgtcat gcttccggca
    12301 tggctgttat tagcaggtaa gccgatgatg atggtcaatc gtccgaagca acagcccccc
    12361 cacgataacg tttagcaaaa aatgctctcc tttatgattt aagagttatg gcgatgatac
    12421 ggggctttac ctccccgtaa tattgcgtta acaggccgct gacggcttcg atcgtgaagg
    12481 agaataacaa tgttcaggtc actgtttctg gcggccgccc tgatggcatt taccccgctt
    12541 gcggcaaacg caggtgaaat caccctactg ccatcaatca aattacaaat tggcgatcgc
    12601 gatcattacg gtaattactg ggacggtggt cactggcgcg accgtgacta ctggcatcgc
    12661 aattatgaat ggcgtaaaaa ccgctggtgg cgtcatgata atggctacca ccgtggctgg
    12721 gataagcgta aagcgtatga gcgtggctat cgtgaaggct ggcgcgatcg tgacgatcat
    12781 cgcggaaaag gccgcggaca tggacaccgc cattaaacac attcgccaat ggagcacaat
    12841 gcctgatgcg acgctagtgc gtcttatcag gcctacaaac cggctcttta acgcgtaggg
    12901 cggataaggc gtttacgccg catccgccaa tagtgccaga tgcgacgctc attcaactac
    12961 aatcccagcg ccgtccccac caacaaccag atattcagcg ccacgaccag cactacaatc
    13021 acccagcctg tctgttttac gcgtttgctg ttcaccagat cgcccatcaa cttgctgtca
    13081 ctggtgaaaa tcagcagtgg aaccagcgcc agagcgatac caaaacttaa cagcacctga
    13141 ctcataacca gaatccgtgt cggatctaat cccatcagaa tgacaataaa tgacggcaac
    13201 atggtaacgg tgcgacgcac ccacagcggg atatggaagc gaatgaagcc ctgcatcacc
    13261 acctgccccg ccagtgtccc caccaccgtt gatgacagtc cggcagcaac caggcttaat
    13321 ccaaagaccg ttgccgcagc atggcttaac aacggttgca gcgtcaggta agcctcatca
    13381 agatcggcaa cgccagtatg accggaaaag tggaacgccg ccgcagccgt agccatcatc
    13441 gccagattga caaaaccggc aatagtcata gcgatagcca catcccattt ggtggcagaa
    13501 taacgttgtt gacgcgaacc accatgtaaa tgctgagtca gcgaagagtg taaataaatc
    13561 acgtgtggca taatcgtcgc ccccaacaca cctgccgcca ggaagactgc ctccgaggtg
    13621 ggtaaactcg ggattaccat tcctttaccc agttgcgcca ggttaggctg ggagaaaatc
    13681 aactcgacaa tgtaagccgc agcaacaaac agcagtaacc cgccaatcac tttctccagc
    13741 ggtttttgcc ctcgacgttg cagcattaaa atcaggaaag tcgcgatccc cgtcagcacc
    13801 gcgccctgca acagcgaaac acccagaatg agtttaaaac cgatcgccgc accaataaat
    13861 tccgccaggt cggttgccat cgcaatgatt tctgcctgaa cccaatagaa ccacactacg
    13921 ggacgcggat agtgatcgcg aatctgctcc gccaggtttt taccggtggc aatccccagt
    13981 ttggcagaca gtatctgaat cagcatcgcc atcaggttgg cccaaacgac aacccacagt
    14041 agctgatagc cgaagctggc acccgcctga atattggtcg caaagttacc aggatcgata
    14101 taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga gcctcatctt gcgcgccgcc
    14161 cgtccgctgc tactctcaac gcgatagtta gtcatcttgt gcctctaaaa catagccttt
    14221 gctatgtttc atgctatgtc aaacgagaat gattatcaaa ttcatttaag tggattgtga
    14281 tgatttcttt gatagaccag aattatgacg acgaaaagat tgatgacttg aatgttgtga
    14341 ttatgtttaa tgttagcaca tttacataac tttcagcttc catacacaac atagcagaaa
    14401 tgtatgacag ctcactattt ttgaagcttg tcacaggacg tcattatagt gtgtgtcaga
    14461 tctcgttttc cttaaccatg ttacatagaa tgtgcacgga aatttaacct gcctcatatt
    14521 tggagcaaat atggaccgcg tccttcattt tgtactggca cttgccgttg ttgcgattct
    14581 cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc cgttatgtta ttcaactgct
    14641 tgttatcgaa gtgttactgg cgtggttctt cctgaactcc gacgttggtt tgggcttcgt
    14701 gaaaggcttc tccgaaatgt ttgagaaact gctcggattt gccaacgaag ggactaactt
    14761 cgtctttggt agcatgaatg atcaaggcct ggcattcttc ttcctgaaag tgctgtgccc
    14821 aatcgtcttt atctctgcgc tgatcggtat tctccagcac attcgcgtgt tgccggtgat
    14881 tatccgcgca attggtttcc tgctctccaa agtcaacggt atgggcaaac tggaatcctt
    14941 taacgccgtc agctccctga ttctgggtca gtctgaaaac tttattgcct ataaagatat
    15001 ccttggcaaa atctcccgta accgtatgta caccatggca gcaacggcga tgtccaccgt
    15061 gtcgatgtcc atcgttggtg catacatgac catgctggaa ccgaaatacg tcgttgcagc
    15121 gctggtactg aacatgttca gcacctttat cgtgctgtcg ctgatcaacc cttaccgtgt
    15181 tgatgccagt gaagaaaaca ttcagatgtc caacctgcac gaaggtcaga gcttcttcga
    15241 aatgctgggt gaatacattc tggcaggttt caaagttgcc attatcgttg ccgcaatgct
    15301 gatcggcttt atcgccctga tcgccgcgct gaacgcactg tttgctaccg tgactggctg
    15361 gtttggctac agcatctcct tccagggcat cctgggctac atcttctatc cgattgcatg
    15421 ggtgatgggt gttccttcca gtgaagcact gcaagtgggc agtatcatgg cgaccaaact
    15481 ggtttccaac gagttcgttg cgatgatgga tctgcagaaa attgcttcca cgctctctcc
    15541 gcgtgctgaa ggcatcatct ctgtgttcct ggtttccttc gctaacttct catcaatcgg
    15601 gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa ggtaacgtgg tttctcgctt
    15661 cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg ctgtctgcgt caatcgcagc
    15721 actggtgctg taagaccata cataaaaaag ccggggataa ttccccggtt ttttacgcct
    15781 gttaatcagc taatggctgc gggcgaccaa tcaaataccc ttgcagatat tgcaccccga
    15841 gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg
    15901 acttcgcttt cgccagatcg gtaattgatc tcacaatcat cgcatccagc gtgttcgtga
    15961 cgatatcttt cacaaagacg ccatcaattt tgatgatatc agcctgcaaa cgctttaacc
    16021 gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc aatccggaaa ccaaacttat
    16081 gcagctgctc gatgttgtac atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga
    16141 tctcaagaat gaccgcctgc ggggagatgt gataacgctt aaacagacga ataatccgcc
    16201 cggcaatatt cttttgcagc agcgtgagcg gcattaaatt gactgaaaag cgcgggcctt
    16261 ttttgtcgca aggatgtgtt gccagccact tcaacaggga ttccagcact tgcaaatcaa
    16321 aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa cttatccggg gtcataatgc
    16381 cgccgtcata tttcagtcgc gcgaggatct catcataacc ttcaccctct ttgttgcgaa
    16441 ttggctgggc gtagagcagc aaatcaccct gatctaacgc cgtacgaatg gtattcagca
    16501 atagcacctg tttagtggtc tgcccggaaa ccatctcctc tctgctatcc agcgccagca
    16561 catggtgatg tgcgcaggat tgctccgcca gccagcttaa ctgccccaac aagggttgca
    16621 gggtttcctg atttccatca aaacgccccc aggcagcacc atagcccata tccagcccgg
    16681 tattgttcca gtgaatttgc cgactattca ggatgttaac catatgctgg agtcgccctt
    16741 ccgtttccgg tccactcagc actaacagca gttcactacc cggcaattga tacaactttt
    16801 cgttttcctg catcaacggc agcagcgtac ggtaaattga tcggatacag tgaacgcgca
    16861 tcattaagcc gtaatgacga ctcataaact caagattatc aatgcgcagg cagcaaaaac
    16921 tcttgcccgc ctcttgctcc ggcgcttgtt ctaacgcacg aaagttgggt aggagcgtta
    16981 acggatcggt cagcgcctgc aaatgccagc ggcgattaag ccattcactg cgatgataaa
    17041 tgcgcaccat atagagcagg caaacgctaa aggaaatcag caccgcgaga ataaatgcca
    17101 gcgaatattc ggttagtacc ccttgcaaaa agttctggtt gtaattcagc aggcaaagcg
    17161 ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag cttaccgaca ccgagggtaa
    17221 agatgataaa gaaaacaggt accagataac cagcaataaa gtcattttca tacggtgtgc
    17281 acatcagaag tagcagcacg ctaagagctg ccagccagct taaggtaaat gcgcgtttct
    17341 ctttgctcaa cgacggagcg atatccctac gccacaatat ctgtgcaaag tggggactta
    17401 caatcatgcg ggcgagatag taaaaaagca tgttgtaaat cagcactgcg gtgaaaaggc
    17461 ttagcaaatc aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggta gatatcttta
    17521 gcggaaaatc aaagaaattt cccacaagat acatgctgca tttgatgcca atcggcgcca
    17581 ccaaaccaag ccagaaaaga cgttgccaga catatcggct ggtcaatcca taacgccaac
    17641 gcgtccccag ctgccagcgt aatattgcgc aggcacacaa caccacaaac gtctggctgc
    17701 cgagcatgac cgcggtctgt aaaaaagaga gatgaaagtt ccacagattg gtgcaaaaca
    17761 tcccgcataa aacggggacg acaccgcgcc agccaaagat aaacaagatt gacagcatta
    17821 cgcacagtgg catccacgcc agaaagatat aactggagtg gataatggca agtggcgaaa
    17881 taaaacggga tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga
    17941 tattttttat caggttatgc tccacaaaca tgagtacact actctcactg atcggggttt
    18001 tcaggcacac aaataataag tttattcgca tcgcgaagca aacaggggcc agcctgttgc
    18061 cgatttttca tccaatgatg ctttcaccag gcggcgctaa cccggacgta gactgtatta
    18121 caaaagcggc aaaaagcaga gacaaaaaac ccccgcttta cagcgagggt ttgaaatttg
    18181 gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc
    18241 tgagctatag ccccacgatg cgtttacgta ccaaatttgc tgggtgcaaa atttggtgga
    18301 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    18361 tatagcccca tcacgtaaag cttgtcgagt tgacgggcgg catcatatga attccgcccg
    18421 aatgtgtcaa cggcaaattg ccacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa
    18481 caattttgga aaaccgctcg cattcagtag ttattcatgt cacggtttcc tgtaaagtgg
    18541 tgttataaaa tgaactacta atagacccac atacattcag ggaattgtta tgttcaagga
    18601 gaggatgacg ccagatgaac ttgccagact gaccgactat agccgccaga ccattaataa
    18661 atgggtacgc aaggaaggct ggacaacgtc accaaaacca ggcgtccagg gtggcaaagc
    18721 tcgactggtt cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg aacgtccaga
    18781 aggtcaggga gaagcgccag ccctttctgg tgatgcgccg cttgaagtgt tgctggtgac
    18841 actggcgaaa gagatgacgc cagttgaaca aaaacagttt acatccttgc ttctgcggga
    18901 agggattatc ggattgttac aacgcttagg gattcgcgat agcaaataat atgaaaagat
    18961 tacgcaataa aatgaccacc gaagaactgg ctgaatgcct cggtgtggct aaacaaactg
    19021 ttaaccgttg gatcagagaa aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg
    19081 gtcgtgccag acttattctg gtcgatacgc aagtttgcga gtttattcag aacacgccag
    19141 ccttccataa cacaccgatg cttctggaag ccgaagaacc ccttgccgaa tacgcaccgg
    19201 gtattcgcac tcctgcttat cggcaaatca tcagcgcaat agacaacatg actcacagtg
    19261 agcaggaaaa agttgcgcaa tttttgtcac gcgaaggaat tcgtaatttc cttgctcgtc
    19321 tcgacataga tgaatcagaa taaataaaaa acggcagggg tatctctcct gccgtttatc
    19381 ttttatgcgc taattattgc tgattttcgc gttcagcaat aaaagccagc gctttgttga
    19441 tacgctcgat actgcgggtc ttaccgattg cgtgaacggt aacatccagc gccggagact
    19501 gccccgcacc ggttacggcg acacgcagcg gcataccaac tttacccata cccacttcca
    19561 gctcatccgc cgtcgcctga atagcgtgat gaacgttttc agcggtccag tcagtaatcg
    19621 cagtcagttt gtcacgaacc acttccagcg gctgacgcgc taccggacgc agatgttttt
    19681 tcgcggcgtc ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca
    19741 tctctttcag cgtcttgcag cgttcgccca gcagtttcac cagatcagcc agctgcgggc
    19801 cgttacgggt atcgatattt tcctgctcaa tgtgccattg taagtgagta gcaacatact
    19861 ccggcggcag cgcgttaatg tagtgatggt tcagccacag cagcttgtcg gtgttgaacg
    19921 cactggcaga tttgctgacg gcattcagag tgaagtattt gatcatctct tcacgagtga
    19981 agatttcctg atcgccgtgg gaccagccca gacgcaccag atagttcagc agtgcttctg
    20041 gcaaataacc gtcatcacga tactgcatta cgctgaccgc cccgtgacgt ttggacagtt
    20101 ttttaccgtc atcgccgttg atcatagaaa cgtgcgcgta aaccggtacc ggcgctttca
    20161 gggctttaag aatgttgatc tggcgcggcg tgttgttgat atggtcttcg ccacggataa
    20221 catgggtgat ttccatatcc cagtcatcaa caaccacaca gaagttatag gttggggaac
    20281 catcggtacg gcgaatgatc agatcatcca gctcctggtt gctgaactca atcgggccac
    20341 ggatctgatc gtcaaaaaca acagaacctt cctgcgggtt agcaaaacgt acaacgcacg
    20401 gttcatcatc agcatgatgc tcatggctgt ggcggcagcg accgtcataa cgcggcttct
    20461 cacctttcgc catttgctct tcgcgcagcg cttccaggcg ctctttagaa caatagcatt
    20521 tataagctgt gccctcttcc agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt
    20581 tggtctggta gtacggacct tcatcccact ccaggctcag ccagttcatg ccatccataa
    20641 tggcttcgat agcttccggc gtggaacgct caagatcggt gtcttcaata cgcagcacga
    20701 actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa
    20761 cgtgcagata gcctgttggg cttggcgcga agcgagtttt gattttcatg aaatggcctt
    20821 acgtttagaa agatgccgac aaccggcaaa tcctggaaaa attgagtggg cgatattcta
    20881 tcactcccgc ctgattcctc aatgtagttc gggttgcaac cttacgcatt attgttttcg
    20941 ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt gcttaaattt acgacgaacg
    21001 aacaatttct ttagaaaatg cgttgactca ttttgaactc tccctataat gcgactccac
    21061 acagcggggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    21121 ttcgatcccg tcatcaccca ccaactactt tatgtagtct ccgccgtgta gcaagaaatt
    21181 gagaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    21241 ttcgatcccg tcatcaccca ccacttcggg tcgttagctc agttggtaga gcagttgact
    21301 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct
    21361 aaaggcgctt ttttgctatc tgcaatttgc gaaattgcct gatgcgcttc gcttatcaga
    21421 ccaactatgt ccggcagttc ccgtgcctct catctactgc gccaacgccg ccagcagttt
    21481 aaccagcgcg ggcgtcacct gctgcgtttc ataaacaata tataagtctg ccgggatgcg
    21541 ttctttgagc ggacggaata tgacaccggg ccagttcatc tgcgcgtagc tgtccgctat
    21601 caatgtgata ccaataccca tactgaccat ggcgagtacc gtttgcggtt cattaacttc
    21661 gcgaataaca accggtgaaa atcccacctg ctggcaaact cgctgcaaaa aatcccagtc
    21721 agtgtacacg ggcggcattg taacgaaata ctcgtcacgt agcgcttcca gcgggacggt
    21781 ggaaaatgat gagagatgat gctcttcagg catcgccacc agaaacgccg attcatgcaa
    21841 ccgtaagctg gtaaaaccag tcggtggttc tgtcgccatt cgccagatcc cggcatcaag
    21901 ttcgcggcgt tccagcaagg ccatttgcat cgcaggcatc ttttcgcgaa acagaacttc
    21961 aacgttagga ttttctctaa ggaatcgccg cataaccggg cgcatccgtc cccacattgc
    22021 cgttcccaca accccgagtt caatccgccc tgcttctccc cgacctattt gttcaacccg
    22081 agccaataca ttatttgcat tcaccagcaa tcgacgcgat tcttccatca agattttgcc
    22141 cgcgtgtgtc agtacgacgc tgcgcgaatg gcgaataaaa agctgcgtgc cgagttgatt
    22201 ttccagctct ttaatatgaa tgctgagcgg aggctgagac atatttaaac gcgctgctgc
    22261 gcgaccaaaa tgcaactctt ccgctacggc aagaaaataa cggagcaact taagatctgt
    22321 tctgtatacg cgttccataa ttaagtgctc catataataa atacatcgta ttaaattatc
    22381 atggcataaa gtatttcacc accagatatc cgacatacat aaacacaatg gataatatac
    22441 ccggtaatac atttggtaca ggaataggca agcgcacgat tgtaaacaaa gcaccaatgc
    22501 caaaaccaac gccagaagaa aataaaatat ctttcactaa ttaaccttta tcataaaagc
    22561 agccccaagg aacagagctg cgaatccttt taatgtgtaa ctgaacgatg cgttatcttt
    22621 tcagggtcat ggttatattt aaacccaaag aaaaatatca ctgcgaggaa aagagcatac
    22681 cctgcaaaca tcagccaaat ggtatgccaa tcttttacgc catccaccga gaagtaatcc
    22741 actgccatac cgctcagaat cgagccaacc catgcgccga caccatttac catggtcata
    22801 aaaaggccct gggcgctggc acgaatgctg gaatcaactt cctgttcgac aaataccgaa
    22861 ccagaaatat tgaagaaatc gaatgcacag ccataaacaa tcatcgacag cagcagcaaa
    22921 ataaacccgg ttggtgatgg gtcgccatag gcaaagaagc caaagcgcag cgtccacgcc
    22981 accatactca tcagcatgac ggttttaata ccgaaacgct tcaaaaagaa cggtatcgtc
    23041 agaatgaaac cgacttcagc catctgtgaa actgacagta aaatggacgg atatttcacc
    23101 acgaagctat cagcaaactc aggattacgg gcgaaatcat gcaggaacgg gttgccaaaa
    23161 acgttggtaa tttgcagcac cgcgcccagc atcatggcga agaggaaaaa gatagccatg
    23221 cgtggatttt taaacagcac gaaggcatcc agacccagct tgctggcaag cgatgtagtc
    23281 gcttttttct ccgcaaccgg aatcttcggc aaagtcagcg cataagccga cagcagcaat
    23341 gacgcaccgg acgcgatata cagctgcaga ctactcaatt ccagatgcag caggcttact
    23401 gcccacatgg cgacaatgaa ccccaccgta ccaaaaacgc gaatgggcgg gaaagcggtc
    23461 accgggtcaa gccctgcctg ggcaagacag gaataagaga cgctgttcga taacgcaata
    23521 gtcggcataa acgccatcgc attgactaac atcacccaaa acatcatatc cggatcagtt
    23581 acggatgccg cataaaaaag tacgcccgca cacaccaggt gacacagcat gtatgcacgt
    23641 tctgcacgca gccatttgtc tgcgatgatc cccattatac ccggcataat aatcgcggcg
    23701 atccctttgg aactgtaaac catgccaaca ttagcgccgg tgaaatgaag ggtattaatc
    23761 atgtaagaac cgagggtaac cagccagctc ccccagataa aatattgcaa aaaggacatt
    23821 acctttaagc gcaaagcgat gctcataatc gtttccttgt cgcatcatat ggcctcactc
    23881 agtaaggccg tttattgttg ttttactcag gcaattttgc gcagaaagcc gcaaataaga
    23941 ttaataaagt tttgctttga gagttccgct gctgccagcg tttgggcatg agaaagcttc
    24001 acgtcactca gaccttccgc catgttggta atcgcagaga ccgcaacgac tttaagttcg
    24061 caatggcgag ctgaaataac ctcaggcacc acagacatac caacaacatc cccaccaata
    24121 atttgcatca tacgaatttc cgccgcagtc tcgaaattcg gccccggata cgagacgaac
    24181 acgccctccg tcagagggaa cccctcttct ttcgccactt tttgtaacag tgcgcggtat
    24241 tccgcatcgt aggcattcgc cagcgagaag aagcgctcac caaaacgttc atcattaaga
    24301 cccaccatcg gcgttcccgg catggtgttg atgtgatctt tcaatgcgac cagactgccg
    24361 gcccccactt caggacgcag tgagccagcc gcattggtgc agaacagcaa ctcgcagccc
    24421 agcaacttaa aggtacggat tgcatccgtc atgatggtca tcccacgacc ttcgtagaaa
    24481 tgtccgcgac ctttcatgca cgccactggc accccctgga gataccccag caccagctca
    24541 cccgcatgac cgtgtacggt acttaccggg aacccaggca gcttttcgta ggaaattgcg
    24601 accgcgttct caatctgatc ggccagcgcg cccagcccgg aacctaaaat aaaggccact
    24661 cgtggcgtga aatcaggttt ataagtcttg ataatatcta cgcaatacag tgggttatga
    24721 gaaaactgag cctgagacat atatatcctt tttttgtagg gtggaatcta acgccttttt
    24781 ataacgagat tgccctacct cataccaata cacttttttc atgtatccga tagttattcg
    24841 atatcgatat atttatattt tttcactatt aataggctga aaaaactgta ttggtactct
    24901 cctttccctt gcattaacaa tatgccgctt ttaatcaacc ttttattaag caatcaacta
    24961 ataacgatcg gcatagggaa ttatgctatg aaaaaacatc tttttactct gacactctct
    25021 tctgtattgg cgataccagc agtatcccat gcagaattta aaggcggttt tgctgatata
    25081 ggtattcatt atcttgactg gactagcaga accactgaga aatcgtcgac caaatcacac
    25141 aaagatgatt ttggatatct ggaactggaa ggcggcgcta actttagttg gggagaaatg
    25201 tacgggttct tcgactggga aaacttttat aatgatcgtc atgataaacc gggtagcgag
    25261 cagcgttata cctttaaaaa taccaaccgt atttacctgg gtgacaccgg atttaatctc
    25321 tacttgcatg cgtatggcac ctacggttct gcgaatcgcg tgaatttcca tgacgatatg
    25381 ttcctttacg gtatcggcta caatttcacc ggtagcggtt ggtggttcaa accgttcttt
    25441 gcaaaacgct atacagacca aacctattat accggcgata acggttatgt cgccggttgg
    25501 gttgcaggtt acagctttat gctgggcagt gagaaattca ccctcaccaa ctggaacgag
    25561 tacgagtttg atcgtgatgc cacctacgcg gcaggtaatg gcggcaaaga aggtctgaac
    25621 ggtgccgtcg cactttggtg gaatgcaact tcacacatca ccacaggaat tcagtatcgt
    25681 tatgcggatg acaaactggg cgaagatttt taccaggatg caataatcta ctccatcaaa
    25741 tttaatttct gattgattac tgccggggct cccggcagta tgaatagctc tatatctgat
    25801 acagcggatc cccttcccgc ccagccatta gcgcacggcc ctggcctcgt accacttccc
    25861 ataacgcttg tgcggcggtg gaaagtgaac ggttcttcct gcgcaccagc atcaactggc
    25921 gttccaccac cggcgtaatg cgctttacca ccagcgggct accttcaggt aacggcagcg
    25981 ccagtgcggg aagaatacta atgccgatgc ctgcggctac catcgggaac agcgtcgccg
    26041 gatgaccaat ctcctgcaca atattcgcct gaataccgtt gcgcgccagt gctgcatcaa
    26101 tcagcggtcg gctgcctgac gcgtaatcct gcaacaccaa tttcgctccc tgtaatgcct
    26161 gccagggtac ataatcttcc gcggccaaag cgctatcgcg atggcaaagc aagaagaaag
    26221 gctcggaaag aatcgcttca cattgcagat cgctcactgg gcctggatcg atgacgatgc
    26281 caaaatcgac atctccctgg cgaatacttt ccatcaccca ctgctgcgga cgatcgtgca
    26341 ggataaactg aatatctgga tagcggcgat ggctttcagc aatgcattgc ggaataagat
    26401 gcgcggaaat ggtctggctg gcagcgaccc gaacttttcc gctcagttgt tgccccatac
    26461 gcccggtatc gcgcaacgtg ctgttaagtt catccagcag tcgctcaaga cgcaaagcca
    26521 actgctgccc tgcatctgta agcaccactt cacgcgtggt tctgtccagc aggcgaacac
    26581 cagtatgatt ttccagctcc ttcacactgt gactcactgc cgactggctc aggccgatac
    26641 gctctcctgc acgactaaaa cttttctcct gcgctactgt gacgaaaacc tttaattgtt
    26701 ttaaagaata attcatcttt tttcttcatg aatggatgca ataaatcaat tttatttctc
    26761 aaactggaag aagcacaata gaaccatcga tcatctggag tctttatgaa actttttcgt
    26821 atcctcgatc ctttcacctt aaccctgatc acggtggtgt tgctggcctc tttctttccg
    26881 gccagaggcg atttcgtccc cttctttgaa aatctgacca ctgcagctat tgccctgctg
    26941 ttctttatgc acggcgcgaa gttgtcgcgt gaggcgatta ttgctggcgg tgggcactgg
    27001 cgactgcatt tgtgggtaat gtgcagcacc ttcgtattgt ttccgattct gggcgtgctg
    27061 tttgcctggt ggaaaccggt aaatgtcgac ccgatgctct actccggttt tctctacttg
    27121 tgcattctcc ccgctaccgt gcagtctgca atcgccttca cgtcaatggc gggcggtaac
    27181 gttgcggcgg cggtttgttc tgcgtcagca tccagcctgc ttgggatttt cctttcacca
    27241 ttgctggttg gtctggtgat gaatgttcac ggtgcgggag gcagccttga gcaggtcggt
    27301 aaaattatgc tgcaattgct actgccgttt gtgttggggc atctttcccg gccgtggatt
    27361 ggtgactggg tgtcgcgcaa taaaaaatgg attgcgaaaa ctgaccagac gtccattctg
    27421 ttggtggttt atacggcatt cagcgaagcc gtcgttaacg gtatctggca caaagttggc
    27481 tggggatcat tgctgtttat cgtggtggtc agttgcgttc ttctggctat cgtgattgta
    27541 gttaacgtct ttatggcacg ccgactgggc ttcaataagg cagatgaaat tactatcgtc
    27601 ttttgtggtt cgaaaaagag tctggcaaat ggcatcccga tggcaaacat tctgttcccc
    27661 acatcggtga tcggtatgat ggtgctgccc ctgatgattt tccatcagat ccaattgatg
    27721 gtctgtgcgg tgctggcgcg tcgatacaaa cgccagaccg aacagttaca ggcgcagcag
    27781 gaaagcagcg ccgataaagc ttaaagcgga cgcttcaggg gctggaccag ttgcgtcagc
    27841 ccctcggttt taatcagcag cgtgatttgc attagctcac cgagcttccc tgccgggaac
    27901 tcatctttgc gggcaaacca cagcagatac tcctccggca ggtcgattaa gcgacgccct
    27961 ttgtacttgc caaacggcat tatcgtattg gctatttcaa tcagctgctc tttttccatc
    28021 tcagctaccc agcaaacgca gcatttccgt ttcgtcgatg acttcaatac ccagttcctg
    28081 cgccttcgcc agtttagatc ctgcagcttc acccgctatc accagatcgg ttttcttcga
    28141 cacgctgccc gcgactttcg cgcccagttc gaccagtcga gctttagcgt catcacgcga
    28201 catctggctt aagctgcccg taagcaccac ggttttacca gcaaacgggc tgtcaatctc
    28261 ttccgcgtta atcaccaccg gcgcaggcca gtgaacgcct tccgccaaca gctcgctgat
    28321 gacattgcgg ttgctttctt cggcaaagaa gttgtgaacg tgggaggcaa cgacaatgcc
    28381 aacatcaggc accttttgca gctcttcaat cgaagcggct tccagcgctt ccagcgtgcc
    28441 gaaatatgcc gccagacctg ctgcggtggc ctcgccgact tcacggatgc caagtgcata
    28501 gaggaagcga gcaaaggtgg tttctttcgc tttttccagc gcgttaacca cgttttgtgc
    28561 cgatttcggc cccatacgct ccagtccggt cagtttgact gcggtgagtt tgaacagatc
    28621 cgccggagtg tggacatatt ctttttcaac cagttgatcg atgattttgt cgcccattcc
    28681 gtcaacatcc atcgcacggc gggaaacaaa gtgtttcaga gactctttac gctgcgcacc
    28741 gcaaatcagg ccaccggtac agcgggcaac cgcttcacct tccacacgct cgacgtcaga
    28801 accacatacc ggacaatgcg tcgggaatac aacctcacgg gtatcttccg ggcgttcaga
    28861 aagcacgacg ttaaccacct gcgggatcac gtcgccagcg cggcgaataa ccactttatc
    28921 gccaatgcgt aaaccaagac gttcgatttc gtcagcatta tgcagtgtag cgttactcac
    28981 caacacgcca gcaacatgga caggttccag acgcgcaaca ggcgtaatcg cgccagtacg
    29041 cccaacctga aactcgacgt cacgcacaaa ggtcatctgt tcctgcgccg ggaatttaaa
    29101 cgctaccgcc cagcgcgggg cacgcgcgac aaagccaagc tgctcctgct gtgccagtga
    29161 gttgacctta atcaccacgc cgtcgatatc aaagcccagc gtcggacggt ctttttctac
    29221 ttcgtgataa aacgccagca cttcttccgc cgattcacaa agtgttaccc gatcactgac
    29281 aggtaatccc cacgctttaa attgcattaa gcgtcccaaa tgggtatccg gcaattcacc
    29341 gccttccaga acaccaacgc catagcagaa gaaagtgagc ggccgcttcg ctgtaatacg
    29401 cggatcaagc tgacgcagtg aaccagctgc cgcattacgt gggttagcaa acactttccc
    29461 gcccgtgcgt cgcgcatctt cgttaatctt ttcgaacccc gcctgcggca ggaacacttc
    29521 accacgcact tccagacgcg ccgggatatt ctctccgtgt agcttcagcg gaatggcgcg
    29581 aatagtacgc acattagacg tgatatcttc cccggtggtg ccatcgccac gggtggcggc
    29641 actgactaaa acaccatttt catacagaat actgacggca agaccatcca gcttcagctc
    29701 acagcaccag gtgactttct cgttgctttt cagacggtcc tgcacacgtt tgttgaaagc
    29761 aagaaagctt tcttcatcaa aaacgttatc cagcgacagc attggcactt catggcggat
    29821 ctggctgaaa gccgccagcg gcgcagcacc tacacgttgg gtaggcgaat caggcgtaat
    29881 cagttctgga tgtttggttt ccagctcgcg caattcgcgc atcagcctgt cgtattcagc
    29941 gtcgggaatt tccggcgcat ccatcacatg ataaagatat tcatgatggc gaagcgtcgt
    30001 tcgcagttct gtcagttgtt gttcgattga ttccatatcg caccatcaat gctaaaaacc
    30061 cccgacaagc gggggttcga agaggagtta atttgcctta agtgtatcag gcgttggcgt
    30121 ctttgacttc gcggatgatg tcctggtact cgcgcaattt ctgcggagtc atcatacggc
    30181 gctgatcgtc aagaacaaca ccgcccactt catcggcaat atgctgcgca gattgcagca
    30241 tcagcttgaa gttctgcagc tcgtcaccgt aagacggtac ctgcataaag atagtgacac
    30301 ccggagtagt gaaatccttc atttcaggat caaaggttcc cggtttcacc atattcgcca
    30361 ggctgaataa cgccgggccg ctgccatccg ggctaagatg acgatggtaa atattcatat
    30421 cgccaaaaat gaagcccgct tgttgaatgc tgttaagaag cagttcaccg tttagctcgc
    30481 taccgtgatg cgccgcgacg ttcatgataa tcaccgcttc tttgcgcttc ggtttatcca
    30541 taactggagc cggttccgct acaggctcag gctgtggtgc cgctacgggt tctgcaggct
    30601 ggaaagcctg ttgtgccggt tgcggtgctg aatgcactgg ctgcggcgct ggcgcgacct
    30661 gtggcgaaac tggctgctgc aacggctgtt caggctgcgg ctgataggca ggctgctgca
    30721 cgggttgctg caccagctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag
    30781 gcggctgctg gaccggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt
    30841 gcggcgacgg acgagcagcc tcatgctcct gggcgttagc cggggcatga ttcacgcggt
    30901 gaactcgaac ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt
    30961 cacgttttga cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac
    31021 ggctggtcca gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta
    31081 atatcagacg caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg
    31141 gcaaacattt actcactaag agtatttgtc gattacctca agtgcaagtg cactattaac
    31201 tttcacagca caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa
    31261 ctgtccattg cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg
    31321 agctcatcct gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt
    31381 ttgcgcaagg ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc
    31441 tgctggtcaa tattttgttg atggggggcg cattctggtg gctctttacg caactcgatg
    31501 tctggatccc gactctcatg agttatgttc cggactggtt acaatggctg agctatctgt
    31561 tgtggcccct ggctgtcatc tctgtgctgt tagtatttgg ttatttcttt tcaacgattg
    31621 ctaactggat tgccgctccg ttcaacggtt tattggctga acaactggaa gcaagactga
    31681 ctggcgctac accgccagat accggaattt tcggtatcat gaaagatgtg ccgcgaatca
    31741 tgaaacgcga atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttgctaattc
    31801 tttacttcat ccctggtatt gggcaaaccg tcgcgccagt actgtggttc ctgtttagcg
    31861 cctggatgtt agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta
    31921 aagagatgcg caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa
    31981 ccagcctgtt tacgatgatc ccgttgctta atctgttcat catgcccgtt gccgtttgtg
    32041 gcgcgacggc gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat
    32101 ctaccggtta ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt
    32161 ccatcttgca tgtcattatt tcccttctgc atatagatat gctaaatcct tacttccgca
    32221 tattctctga gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc
    32281 atgagtaaga tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg
    32341 aatcgcatcg gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc
    32401 gttaagtgcc gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa
    32461 ccaggcgttg aactggttga accaaccagc ggtaataccg ggattgcact ggcctatgtc
    32521 gctgccgctc gcggttacaa actcaccctg actatgccag aaaccatgag tattgaacgc
    32581 cgcaagctgc tgaaagcgtt aggtgcaaac ctggtgctga ccgaaggtgc taaaggcatg
    32641 aaaggcgcaa tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg
    32701 ctgcaacaat tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag
    32761 atctgggaag ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt
    32821 acgctgactg gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct
    32881 gtcgccgttg agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt
    32941 aaacctggtc cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taaccttgat
    33001 ctcaagctgg tcgacaaagt cattggcatc accaatgaag aagccatttc taccgcacgt
    33061 cgtctgatgg aagaagaagg cattcttgct ggtatctctt ctggagcagc tgttgctgcg
    33121 gcgttgaaac tacaagaaga tgaaagcttt accaataaga atattgtggt tattctgccg
    33181 tcatcgggtg agcgttattt aagcactgca ttgtttgccg atcttttcac tgagaaagaa
    33241 ctgcaacagt aatgccagct tgtaaaaaat gcgtaaaaaa gcaccttttt aggtgctttt
    33301 ttgtggcctg cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta
    33361 tttaaccaga ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg
    33421 aatcgatttt atgatttggt tcaattcttc ctttagcggc ataatgttta atgacgtacg
    33481 aaacgtcagc ggtcaacacc cgccagcaat ggactgtatt acgctcttcg tgcgtcgcgt
    33541 ctgttaaaaa ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt
    33601 tccacaacac taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta
    33661 ccgctccgaa cggtctgcac acccgccctg ctgccctgtt tgtaaaagaa gctaagggct
    33721 tcacttctga aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta
    33781 aactgcagac tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag
    33841 acgagcagaa agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg
    33901 ggttctttta aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc
    33961 cccgggtatc gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg
    34021 gaaaaaaatt tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc
    34081 caaggcatca gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga
    34141 aaaagaagcc atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga
    34201 aatcatagcc ctgattaaag ataagcacat gacagctgac gcagctgctc atgaagttat
    34261 cgaaggtcag gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc
    34321 tgacgtacgt gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga
    34381 cctgagcgcc attcaggatg aagtcattct ggttgccact gatctgacgc cgtccgaaac
    34441 cgcacagctg aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc
    34501 ccacacctct atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt
    34561 cacctctcag gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta
    34621 cgtcaatcca accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc
    34681 tgaaaaagca gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca
    34741 ggtagaagtg tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg
    34801 cgctgaaggc gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc
    34861 cactgaagaa gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc
    34921 ggttatcgtt cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc
    34981 gaaagaagag aacccgttcc tcggctggcg cgctatccgt atcgcgatgg accgtaaaga
    35041 gatcctgcgc gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat
    35101 tatgttcccg atgatcatct ctgttgaaga agtacgtgca ctgcgcaaag agatcgaaat
    35161 ctacaaacag gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt
    35221 aatggtggaa acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt
    35281 ctttagtatc ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga
    35341 tatgatttca cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt
    35401 tattgatgct tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgcaggcga
    35461 tgaacgtgct acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat
    35521 ttctatcccg cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt
    35581 agcagagcag gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt
    35641 cattgaagaa aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga
    35701 agatcatggg tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg
    35761 gaactattga gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg
    35821 atgtcgtttt tgcggaaaaa atcgttggtg atggcattgc tatcaaacca actggaaaca
    35881 aaatggtcgc gcctgtagac ggcaccattg gtaaaatctt tgaaaccaac cacgcgttct
    35941 ctatcgaatc tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac
    36001 tgaaaggcga aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata
    36061 ctgtcattga atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg
    36121 ttgttatctc caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtgactg
    36181 tgggtgaaac cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc
    36241 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
    36301 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
    36361 ttaacgcctt ccctttcagc aatccactga taagctgagc acaaaacagg tcgccagtcc
    36421 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
    36481 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
    36541 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
    36601 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc gccagcggca
    36661 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
    36721 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
    36781 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagctc
    36841 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcat
    36901 ccggaatcgc accaccgtag aaagtgtcat aatgcggtgt attgctcagc aataccgtcg
    36961 gcacggcaaa gacattcagg ccgttctgtt tgatagcagg caccgcaatg ctgttgccta
    37021 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcaat gccctgctct
    37081 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
    37141 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
    37201 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cgctgctaat gacactcccc
    37261 gtttacgcga agttaactgc ccatgaagaa gcccgtatca acgccatgct ggagggatta
    37321 gcacagaaaa aggatttgat atttgtgcgt aacggcgatg atcatacctg cgatgaagcg
    37381 gtttcccacc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
    37441 tttattgata aggtagcctc gtcgtcatcg attaccggga agccgtatat cgtgaagatg
    37501 cccggtaaaa gcgatgagaa cgcacaacct tttttacatg cgctgattgc gcagacggat
    37561 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct ggctaaaatg ctcttcccca
    37621 aacaccccgg tagaaaggta gcgatcgccg cgatcgcaga tgatcgccac aaccaccgcg
    37681 ccggggttag cttttgccac gcgcagtgct ccggcaaccg cgccgccgga gctgacgcca
    37741 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttccgcatc gcgctgatga
    37801 atatccagca cctcatccac cagcgaagcg ttgaaaatcc ccggcagata ttccgcaggc
    37861 cagcggcgaa tgccgggaat gctactgccc tcttccggct gcaggccaac aatggtcacc
    37921 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
    37981 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtggtg
    38041 gtgtaatggg cgaagggatt atcaggatta ttgaactgat cgagcagctt tccttcacca
    38101 cgattggcca tctccagcgc cagatcgcgc gcgccttcca tgccctgctc tttggtgaca
    38161 agaatcagtt ccgcaccgta tgccagcatc gctgcacggc gctcctggct catgttgtcg
    38221 ggcatcagca atttcatgcg atagccttta agcgcggcaa tcattgccag cgcaatgccg
    38281 gtgttaccac tggtggcttc aatcaggaca tcgcccggtt tgatttctcc gcgcttttcc
    38341 gcctcgacga tcatcgaaag cgctgcacga tctttcaccg aacctgccgg gttattgcct
    38401 tccagtttca accagacttc actgccgtta tccggcgcca ttcgctgcaa cttcaccaga
    38461 ggcgtattgc caattgtttg ttctaatgta ctcacgatct ctgtccatac gtggtgttgc
    38521 ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc cattaaaaag
    38581 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
    38641 tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat acagccgcgc
    38701 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
    38761 aaccgtcagc ggttcgttgt accaccccag cggctgcacg actaattggg tgtagtggcc
    38821 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgaggctgg tacggcggct
    38881 gatatccact tcccacggac gcaggaagag atccaccggc ccctgatacg caggtgtata
    38941 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
    39001 gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc gccatacctg
    39061 gtccggtgca tcagcctgct cgatattgcc ctggctcatc accactacac gatcggctac
    39121 ttcggtcgct tcttcctggt catgggtcac aaacacgctg gtgaatttca gctcttcatg
    39181 taactgacgc agccagcgac gcaactcttt acgcacctgc gcatccagcg cgccaaacgg
    39241 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagtg ccacacgctg
    39301 tttctgaccg ccggaaagct gtgctggata acgatccgcc agatgcgcaa gctgcaccat
    39361 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg
    39421 cagcaccgtc aggccgaaag cgatattgtc gaacaccgtc atatggcgga acagcgcata
    39481 gtgctggaac acgaaaccga ctttacgatc gcgcgcatgc aggcggctga cgtcggtgcc
    39541 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
    39601 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag gaatatccag
    39661 tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat
    39721 gctcatgatg ttcctcctgc tgcgcgcgtt tttcctgatt ctccagacgc cactgcaaca
    39781 tactctttaa aaacagggtg ataatcgcca tcagcgtcaa caacgcggca gcggtaaagg
    39841 agccgacggt gttgtagtct tgctccagca attcaatctg taacggcagc gaaagtgttt
    39901 cgccgcgaat cgagccggaa accaccgaca ccgcaccaaa ctcgccaatc gcgcgggcgt
    39961 tggtcagcac cacgccataa agcagcgccc agcggatgtt cggcagcgtg acgcggcgaa
    40021 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
    40081 ttaacatcac cggcaccagc tcacgcacca caaacggaca cgtcacgaag atggtgacca
    40141 gcaccattcc cggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
    40201 gcgggccgtt agagccgtag aacagcagat acaccagacc agcaaccacc ggcgacacgg
    40261 caaacggaat gtccaacagc gtcagcaata gctggcgtcc ggggaagtta aagcgcgtca
    40321 ccagccaggc cagcagaata ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
    40381 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
    40441 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
    40501 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
    40561 cataacgctt caactgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg
    40621 actttgcaga gtgttaattg agaacagcag cagcagcgat gccgcgagga tcaccgaggc
    40681 aatcgcgctc gccgccgggt aatcaaactc ctgtaagcga acaaagatca tcagcgacgt
    40741 cacttctgtt ttccaggcga tattcccggc gataaaaatc accgcgccaa actcaccgag
    40801 gctgcgggta aacgacagcg ccacacccgc caccagcgcc ggagatagtt ccggcagcac
    40861 cactttgcga aaactctgcc agcgtgttgc gccgagcgtt tctgccgcct cttcatattc
    40921 cgggcctaac tcttccagca ccggctgcac ggtgcgcacc acaaacggaa tgctggtaaa
    40981 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
    41041 ccattcaccg taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc
    41101 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcgac ccggaaagcg
    41161 atagcgggtt aagatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac
    41221 aaacgccgac agcagcgtca ctttataggc tgcgaccacc tgcgggttgg tgatcacctc
    41281 ccagtactgc gcccagctca tctgggccag ttgcatcacc agcgcggaga gcggcagcag
    41341 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
    41401 gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg
    41461 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt atcttccacg
    41521 cggaacagct cggtctgcgg gaatttatct ttcagtttgc ccatgacttc cgggttattt
    41581 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttcagg
    41641 taggcttttg ccgctttttc cgtgccgttg gcctgtacgt ttttatccac ccacgccacc
    41701 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcatac
    41761 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
    41821 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
    41881 aactgggtca taaactgttc ggttttggct ttatcaccac cgtcggcttt gtccgctgcg
    41941 ccccatgccg caaggtaggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
    42001 agcttcacgt cagagcgtac caggtcgttc cagtcgtgga tattctttgg gttgccctta
    42061 cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc
    42121 cagtccgctg ggatcagctt gcctttatcg tgcaggattt gtacgtcggt cacctggtta
    42181 taagtgacaa cgtcggcttt taaaccctgc aaaattgcca gcgcctgttt tgatgatccg
    42241 gcatgagatt gtttaatcgt cagtttgtcg ccaccgttat cttttgccca ttgttgctca
    42301 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
    42361 tccgttgcct gtacatggcc cgccagtagc agagaagcaa ccagcacgag tgagttcttt
    42421 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
    42481 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
    42541 tgaaaaggga ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca
    42601 gatgccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc
    42661 ggttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg
    42721 atcggcgagg cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc
    42781 ttccgggttc gactggcggg caatgctttc cgccattggc gtgcgaacgt atccagggca
    42841 gatggcgtta acgcgaatac cagactgcgc gtactccacc gccagcgatt tagtcaggcc
    42901 aacaatcgcc gctttcgtta aggcgtacgc cgtttcgccc gggtctgcca ccatatcgcc
    42961 agtgactgaa gacatcatca caatccggcc atctttgcgg gcaatcatct ccggcagcac
    43021 cgccttcgtg acgttccata cgccttttat attgatatca atatggaaat cgcgatcttc
    43081 atcgctcata tcaaggaaac tgcccagacg acaaacgcct gcgttattca ccagaatatc
    43141 aatgcgccct tctttttcct tagcgcgctt aatagctgcg gcgaccgacg ccgggtcacg
    43201 cacatcggcg acaaccgccg tacagcgatg accacggcca cacagttcgt ccgccagctt
    43261 ttcgatctca ggggagatat ccagcaagat taggtttgcg ccatgacgcg caaaagttct
    43321 ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag
    43381 tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa
    43441 atacaggata aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc
    43501 agtcgctgct ttatgcatca tttcaggcgt tgcgttagct cactgctgcg ctcaatcatt
    43561 ttcaatgact ccacatcatt gagttgcaca aggccaacaa acagtaaatc ggtaacagaa
    43621 ttttgcgccg tgcgggtgga catcgacgag ctgcgccatt cggtttcgcc agaaacggta
    43681 tcgagggtgt aatgcgccag ccgccgcagc ggggaatcgg ttaatgaggt aatggcaatc
    43741 accgttgccc cctgctttcg cgctgcctcg gcgcacaaga caatctcttt tttactgcca
    43801 ctgtaagaga tagcgatttg tacatcgcct ttttttagcg cctgagaaac ggtggcttgt
    43861 acgtgggtgt cggcctcaca ggccacgcga taaccaattt tcatcagttt gaacgacaga
    43921 tcgcgcccca ccagctccga cccgcccagg cccgttatct ggataaacgg tgctttactg
    43981 atgacctcaa tgattttttg cagccgcgcg tagtcaaaca acgcgcaggt ttgctccagc
    44041 gcctgctctt tttcgcgatt cagtttacgg gcaatcactt ccagcgaatc atcactggta
    44101 atcgaactgt gcaggtgcag cgccgtggca ttcgttttct cccggctggc actgtattcg
    44161 ccaattaacg ccatacgcaa ttcagtaaat ccttgcgcgc cgagcttctg ggcaaacttc
    44221 acaatactcg actggctgat gccgagctgt ttcgccatct tgcgcgaaga gacggatttc
    44281 agctcactca cgttggcccg cagaaagtca gcaatttttt gctcgttccc ggtaaattca
    44341 ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctgg gctggcagca
    44401 aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt
    44461 cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa
    44521 atagtaaggt caccaccgat gcaacttgaa aagatgatta ccgaaggctc gaacgccgct
    44581 tcggctgaaa ttgaccgcgt atcaacgctg gaaatgtgcc ggattatcaa cgatgaagat
    44641 aaaaccgtac cactcgccgt tgagcgcgta ctgccggata tcgccgcagc gatcgatgtt
    44701 atccacgccc aggtcagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatcaggt
    44761 cgtctgggga ttctggatgc cagcgaatgt ccgccgacct acggcgtgaa accgggtctg
    44821 gtggttggat taattgctgg cggcgaatat gccattcagc acgcggtgga aggcgcggaa
    44881 gatagccagg aaggcggtgt gaatgatctg aaaaacatcg gattaaccgc gcaggatgtg
    44941 gtggttggta ttgccgccag tggtcgcacg ccgtatgtga ttgccggact ggaatacgcg
    45001 cgccagctcg gctgccgcac agtgggaatt tcctgtaatc cagggagcgc ggtttccacc
    45061 accgctgagt ttgctattac gccgatcgtt ggtcccgagg tggttaccgg ttcttcacga
    45121 atgaaagcag gcacagcgca gaaactggtg ctcaatatgc tctccaccgg gctgatgatt
    45181 aaatccggca aagtgttcgg caacctaatg gtcgatgtgg tcgccaccaa cgaaaaattg
    45241 catgtgcgcc aggtcaatat cgtcaaaaac gctaccggat gtagcgcaga gcaagcggaa
    45301 gcagcgttga ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat
    45361 ctcgatgccg cagaagctaa aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt
    45421 ttagacaagg aataacccat ggccaaagag atcagcagtg aacttctgaa caccattctt
    45481 acccgggtcg gtggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctgcgt
    45541 ctgggtgtac atgacagttc actggttgat cccaatatca aaacgctgga aggcgtgaag
    45601 ggcgtcattt taaccagcga ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt
    45661 gccgcaaaag cgatgagcga gctgctggga gaagcaccgg tacaggatgc cgcagaaatc
    45721 gccgcccaga acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc
    45781 gccaaattcg ccaccatctt cacgccgctg atccccggtt ttattgccgc tggtctgctg
    45841 ctggggatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggcacg
    45901 ctacccgatg cgctgaattt tatgaaggtg ttcagcaaag gcctgttcac cttcctggtg
    45961 attctggtgg gctataacgc cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt
    46021 atcgccgcgc tctttttgct cggttataac ccaactgcaa ccaccggtta ctacgccgga
    46081 tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgttgatt
    46141 gccgcgtggg cctgcgcccg catcgaaggc atggtgcgcc gctttatgcc ggacgatctc
    46201 gacatgctgt tgacctcgtt aatcaccctg ctaatcaccg ctacgcttgc gtacctgatc
    46261 attatgccgc tgggcggctg gctgtttgaa ggcatgtcgt ggctgtttat gcacctgaac
    46321 agtaatccgc tcggttgtgc agtattagcc gggctgttcc tgatcgccgt ggtgtttggc
    46381 gtgcatcagg gctttattcc cgtttacctc gcgttaatgg acagtcaggg attcaacagc
    46441 ttatttccca tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac
    46501 tggcgggcgc aaccgcacag tgcgctacgc agccaggtgc gcggggcgat tattcccggt
    46561 ctgttgggcg ttggcgaacc gctgatttac ggcgtcaccc tgccccgcat gaaaccgttt
    46621 gttaccgcct gtttaggtgg cgcggcgggt ggtttgttta tcgggctgat agcctggtgg
    46681 ggcctgccga tgggcttaaa cagtgccttt gggccgtctg gtctggtggc gctgccgctg
    46741 atgacatcag cacaaggtat ccttccggca atggcggttt atgcaggcgg gattctggtg
    46801 gcatgggttt gcgggtttat tttcaccacg ctctttggct gccgtaacgt caatctggac
    46861 tgataatatg aaacggacaa tgctctatct ttctctgctg gctgtcagct gtagcgtcag
    46921 cgccgcaaaa taccctgttc tgacagaaag ttcgccagag aaagcagggt ttaacgtcga
    46981 acggcttaat cagatggatc gctggataag ccagcaagtg gacgccggtt atcccggcgt
    47041 aaacctgctg attataaaag ataatcagat tgtttatcgc aaagcctggg gcgcggcgaa
    47101 aaagtacgat ggcagcgcgt taatggcgca gcctgtcaaa gccaccaccg ggacgctgta
    47161 tgacctggct tccaatacca agatgtacgc cactaacttc gccctgcaaa agctgatgag
    47221 cgaaggcaaa ctgcatcctg acgatcggat tgcgaaatat attccagggt ttgccgacag
    47281 cccgaacgat gcgatcaaag gcaaaaacac gctgcggatt tctgatctgc tgcatcacag
    47341 cggcggtttc ccggcagatc cgcaataccc gaataaagcg gtcgcgggcg cgttatattc
    47401 ccaggataaa ggccagacgc tggagatgat caagcgcaca ccgctggagt atcaaccctg
    47461 cagcaagcat atctacagcg atgtcgatta tatgctgctt ggatttatcg tcgagtcggt
    47521 taccggtcag ccacttgacc gctatgttga agagtcgatt tatcgcccgc tcggcctgac
    47581 gcacacggta tttaatccgc tgctgaaagg ctttaaacca cagcaaattg ccgccacgga
    47641 actgaacggc aatacccgcg atggcgtgat tcacttcccg aatatccgca cttccactct
    47701 ctggggtcag gtgcacgatg aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc
    47761 aggtttgttt tccgataccg gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg
    47821 tggctatggt gatgtgcagc tgttcaatgc ggaaacggtg aagatgttca ccaccagctc
    47881 taaggaagat gccacttttg gccttggctg gcgcgtgaat ggtaatgcca ccatgacgcc
    47941 gacgtttggc acgctggcaa gcccgcaaac ctacggtcac actggctgga ccgggacggt
    48001 gaccgttatc gatccggtga atcatatggc gattgtgatg ttaagcaaca agccacattc
    48061 gccagtagcc gatccgcaaa agaatcccaa tatgtttgaa agcggtcagt tgccgattgc
    48121 aacttatggt tgggttgttg atcaggtgta tgcggcgtta aagcagtaat aaaaaggcca
    48181 gtcagttgtg atactggctg gcctttaatc atcgaatcag aaacgcttac aacgccatta
    48241 acttgtccag cgacggcgcg aagtaatagc cgccagtgac cggtttggta aaacgcaaca
    48301 tcgcatcacg tttgccatcg gtatcgccaa acatgctcag cagttgctgc tcaatgttat
    48361 gcagacgcgc gcagtaggcg cagaagtaca gaccgtgcgt accgctggcg gtgccgtacg
    48421 gcaggctctg tcgaacaatc ttcagccctt tgccatcttc tttcagatca acgcgggtca
    48481 ggtgagaggt ttccggacgt tcgtcgccgt caatctcttc gttggcctct ttagtgcgcc
    48541 cgatcatcat ctcttgatcg tgaatgctca tccggttgag ttgcttcagg ttgtgctccc
    48601 aacgctgcac aaacacatag ctgccgccgg catccacgcc atctttgata accgccactt
    48661 cgcggcgcgt ctcttcaccc gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac
    48721 gctcttcaac ccaacggaag ccgtggatct cttctttcac ttcaatgcaa tcaccaaacg
    48781 cttccatcgc cgcctgggcg acagagaagt ttacgtcgtg acgcagagaa agaatgtgga
    48841 tcaacacatc gaactgggtc gtcggtgcaa gacctttacc gtagcccgga aaatctttca
    48901 gctcttcggc cccaacgccg ccgctcagag cgcgccaggt attgttacca aaggcaacca
    48961 ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt tgccagcttg tcggcaaatg
    49021 ttttactggc cgcacgcagg gcgtcaactt cccctttcac gttggcttcg atccaaatcg
    49081 ccgcgcggca atgttctggc aaaatgccac tctgaacctg agacattgtt cctcctgaaa
    49141 ataagaatgc cacgcaatcc gtggcattgt tgggcgctat tttacgtgtt ttttcgcggc
    49201 ggcggtttgt tcagacgcaa attaacgccg ccagataatt ttactgactt tccagttttt
    49261 cagggtatcg tccgaaggca ttaacccttc aggaccactc cattccccgc tgaactggta
    49321 gctaatatgt tgactgcctt cggctttaca ttcgacggca cgattatcat caccatcagc
    49381 tttttggcaa ttgccaaatg ctttgctgta aaggtcgcta aacggtgtac cgattttaac
    49441 accggtgtca gcaggaatat cgctatccag cacgtcaatg cgactgatcg tgccttgatc
    49501 gccgttaatc accatcgcca cgttgtcgcc cttcatcact tcaaaaaagc gcaccacgtt
    49561 gccgttcgcg gttttcattc cgctgcgcag gcgataatcg ccatcaagcg catcggcaat
    49621 ggcttgttct tgcagtggtg tggacgccgt taattcacca acgccctgct cgctcacttt
    49681 ggtggatgat ccaaaccagt tccacggatt agcggcagac cagttaacgg acgacatcgt
    49741 ggaacagccg gtcagcatca acggcatagc gcataacatt aaacgcagcg atttcatgtc
    49801 acttcctttg gttattcaat aacgttgctt ggagtgcaaa ttcaccaaaa agtgccgtta
    49861 ttctttttct tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc
    49921 cacaatatcc gccactacca gcgccagacc gataccgcta acggattcac cgttcagcca
    49981 caaccacggc tgccagcaaa gtaaaaccac ttgcgccaac agcaacagaa aatagagcac
    50041 atgccagata cggggaaatg aagcccgccg accactcagc aaaaacgcca gcaccgccgg
    50101 aatgccggga atcaacccca gccagaaatt atcgtgatcg ggataaaaca gatttagcag
    50161 tgcagtaccc tgctcgcgcg acgcaccggc aatgacaaac agcacccagg ttcgcgcctg
    50221 aagcaatagc acaagccaga agagcaaggg taaacgcagg cgaccgtacg catcataatg
    50281 gacaggatga aactcagtac tcttcatctt caatcaaacg cttacccaga ctcagcacgt
    50341 cggcgtgttc atatcccagg cgttcataca ttccgagcac catgtcgtta tcttccggca
    50401 cgttgatctg aattttcggg cagccgcgag caatcagctt tttctccaac cgattaagca
    50461 acgcattagc aatcccacgc ccacgaaact ctggatgcac gccaagataa taagcagacc
    50521 cgcgatgccc gtcataaccg cccatcaccg ttccgaccac ttcaccgttc acctcagcga
    50581 ccagaaacaa actgacgtca tggttcatct tacgctcaat gtccatttcc ggatcgttcc
    50641 acggacgcag caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc
    50701 gaaatacgcg tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg
    50761 cgtgaacggt cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg
    50821 gagttgtcaa ctattgaaat gaaaagtaaa acaattctca acagcaaacg gtcgtaacgg
    50881 attacgcgat acgatataac atctggaact ttattattac aactcaggcc gtatgagcac
    50941 ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagcag gacttgcagc
    51001 gctgacgttg tcaggaatgt cgcaagccat cgccaaagaa gaacctttaa aaaccagcaa
    51061 cggacacagc aagccgaaag caaaaaaatc tggtggcaaa cgtgtcgttg ttctcgatcc
    51121 aggccacggc gggattgata ccggagcgat cggacgcaac ggttcaaaag aaaaacatgt
    51181 ggtgctggca attgccaaaa acgtccgttc cattttgcgt aatcatggca ttgatgcgcg
    51241 tttaacgcgt tctggcgata cgtttatccc actgtacgat cgcgttgaaa tcgcccataa
    51301 acatggcgca gatctgttta tgtcaattca tgccgacggc tttaccaacc cgaaagctgc
    51361 cggtgcttcg gtatttgccc tctctaaccg tggggcaagt agcgcaatgg cgaaatacct
    51421 gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa aaggcgactg acaaggatca
    51481 cctattgcag caagtgctgt ttgatctggt gcaaaccgat actattaaaa acagcctgac
    51541 gctcggctcg catattctga aaaagattaa gccggtgcat aaactgcaca gccgcaacac
    51601 cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt ccttcggtgc tggtggaaac
    51661 ctcgtttatc accaacccgg aagaagagcg gctgttaggc acggcggcat ttcgccagaa
    51721 aatcgccaca gcgattgccg aaggcgtgat cagttatttc cactggtttg acaaccagaa
    51781 agcacactcg agaaagcggt aagtgatgaa acccgacgca caccaggtta aacagtttct
    51841 gctcaacctt caggatacaa tttgtcagca gttgagcgcc gtcgatggca cagaatttgt
    51901 cgaagatagc tggcagcgcg aagctggcgg cggcggacgt agccgggtgt tgcgtaatgg
    51961 tggtgttttc gaacaggcgg gtgtcaactt ttcacatgtc cacggtgagg cgatgcctgc
    52021 ttccgccacc gctcatcgcc cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc
    52081 actggtagtg catccgcata atccgtatgt tcccaccagc cacgcgaatg tgcgcttttt
    52141 tattgccgaa aaaccgggtg ccgaacccgt ctggtggttt ggcggcggct tcgacttaac
    52201 ccctttctat ggttttgaag acgatgctat tcactggcac cgcaccgccc gtgacctgtg
    52261 cctgccattt ggcgaagacg tttatccgcg ttacaaaaag tggtgcgacg actacttcta
    52321 cctcaaacat cgtaacgaac aacgcggcat tggcgggctg ttctttgatg atctgaacac
    52381 gccagatttc gaccactgtt ttgcctttat gcaggcggta ggcaaaggct acaccgacgc
    52441 ttatttacca attgtagagc gacgtaaaac gatggcctac ggcgagcgcg agcgcaattt
    52501 ccagttatat cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc gtggcacact
    52561 gtttggcctg caaaccggcg ggcgcaccga gtctatcctg atgtcaatgc cgccgctggt
    52621 acgctgggag tatgattatc agccagaaga tggcagcccg gaagcggcgt taagtgagtt
    52681 tattaaggtc agggattggg tgtaactccc tcccccccat tcccgcatcc gctgatgcag
    52741 cgtcagtgac ggcttctcgg caaacagctg ctggtaatcc gtggcgaatt gccccagatg
    52801 ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc aagggcttat
    52861 cagttcgcgg cgtacggcgt tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc
    52921 taaaatagcg tgaaacgcgt tttgtagcgt gcggcggctg acatgcagtt gattacacaa
    52981 gtccagcacc gtcactggtt cggacatgtt ttccagcaca tattcacggg cgcgggaaag
    53041 caatcgacgg taactctgat gactgatgct ttccgccgtc accattggct gcgcttcttc
    53101 cagcatcgcc cccatcgcca ttagcaaatt atcccccagc acttttcgca ctgcaggctg
    53161 atggagattt tccgggtttt cgcaaaacgt cgccagcgcc tgttggacaa agccccacag
    53221 agcggcttta tgctgctctt tcacttccaa cgccgactgg ctacgcagca tatgtaatac
    53281 ccgatccgga ttatgcaaaa agttagcctg acgagtgatg acatcttcag aaagcactac
    53341 gccaaggatc gtgtaatcgt ccggcgtact taattcaaac tcggttccac cggggcgcgt
    53401 cgctatttct gcgcttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc
    53461 cggaatgcca aaccagaacg agttcggcca gaccaggcac gactggcgca gcgccagacc
    53521 ggtgtattca cggaaaacct gaatatcatc gagtagaatt tccgtaaatt caccatgaaa
    53581 cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg gtaattgtca aagcatgttc
    53641 atagacatcc gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt
    53701 caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt
    53761 catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt
    53821 gagacgataa cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc
    53881 atttctggct ttttccagcg gccagccaaa gtgggcagcc acttctcctg ccgtcattcc
    53941 ctgacgtact gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg
    54001 cgcgtcgggt tcctttacgg gttgcttagg ctggcggtta aagccagtaa ccagccactg
    54061 ggtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc
    54121 ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag
    54181 cgttaggcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag
    54241 catggcatca gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc
    54301 attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac
    54361 tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc
    54421 tgccgagtag ttggtttcag acggtggcgg gacataggtc accagctgta cgtcggcaga
    54481 tttcagggct gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat
    54541 cggatcgcca agcgtgatcc cggcggttga tgagagataa gaaccggtac gcgaaactac
    54601 atgtgccagg aaggctgtgt tttctgcgtc attagcccac tggaaagcag cgccattttc
    54661 aatatgcgca accatcgcat ccagaccagc acgcacttcc gccgggtttg gcccaccaag
    54721 cataatcagc acttcaccgg cagtcggtga cgggccgtgt gcggctcccg catacagcga
    54781 gcggccatac accacttcca ccatcgcctg cttggtcgct tcgtcagccg caatgtaggt
    54841 gacgtcatct gaatcagcag aaatcagccc gaggctacga atatgcggcg gcaatttcag
    54901 ttcacgcgca aattcggcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg
    54961 aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt
    55021 ctgctccagc atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt
    55081 gccgccctgg tgaatgttag agatacaggt acggtcggcc tcaacggtgg ttgccatacg
    55141 cggcgagtaa acggcgtagc aggagaggct ttctgactgc cctaatcccg ggcgttcccc
    55201 caccagtagg atcactactt tcgcgccgag gatctcaccg atctgatctt caatcttcac
    55261 gcgaccataa cgaacaaaga atggtgtacc gactttcagc ccggcctgtt tcaggcccgc
    55321 catcagcggt ggcaggatct cttcgtagtt cacggtgatc gcatcagttg acaggccatc
    55381 agaaataacg acctgtacgt ccggattagc aacacactgt gctttcagcg cttcaacagc
    55441 ttctgcacac aggcggcggc ccatatccgg acgagtcagg tagagatttt tgtcgctaat
    55501 ttcagagcgg acttccagca agccctgcgc tttcacccac tcttccggta cttctttcag
    55561 tacggtatct ttcgaacgag agtgatcggc caggaaacgc agcaacgcct gggtacgcgg
    55621 acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa
    55681 tacgtctgcg cgatgcgggt tttccacacc aatccacgct tttgcttccg cagaacctaa
    55741 atccagcgcg cagctttccg aggtcactgg tgtcgtacag gtggtggtgg cgcactttgc
    55801 ttctgacggc gcgggggccg cttgtcccat tgacgccatc acgctgcgta caatttcttc
    55861 aatctgtttt tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg
    55921 cccgcccgtt tggtcaggcg accgtttgcc ataatgccca tgctttccag ccagcgttca
    55981 aactccggtg acgggcgcag gttgagtaac tgacgcacag tggcggtgtc gtggaaggcc
    56041 gtagtctggt agttgagcat gatgtcatca cccagcggca tccccatgat gtagttacag
    56101 cctgcggtgg cgagcaggat catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg
    56161 ttggtgtagc agcagtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct
    56221 tctaagcccg cgcggataat ctggcggtcg ttgtagagat actccggccc aataaaaccg
    56281 accacggtgt tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt acgcgcttcc
    56341 atcgtcacct ggtcagcgcc gaagttagcg ccagcggaca gcgccgaacc ttgtccggtt
    56401 tcgaagtaga ggcagttttc cccggcgata cggttaaact ctgcacccac tgcgcgcgct
    56461 tcgtcgagca tcgccagctc cacgccaaac tctttcagcc ctttttcgct gccgcaaata
    56521 ctctggaaaa tcagcccgcc cggtgcgccg cgacgaatcg cttcgatctg ggtggtgacg
    56581 tgcgccagta cgcagccctg agttgggatg ttgaatttgt cgatcacgcc atagatggta
    56641 tccaacacgc ggcttaagtt ttccacgtcg tcagtcaccg ggttaacgcc gatcaccgca
    56701 tcgcccaccc cgaaggaaag cccttcgtag atttgcgcgg cgatactttg cacatcgtca
    56761 cgggtatcgt ttggctgcaa gcgggcgcta aaggtgcccg gaataccgat ggtggtattg
    56821 gcctttttga tcaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc
    56881 ttcgctaccg ccgctaccac ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc
    56941 acgctggttt catcgctcag cacatactca cgcagttcgc tgatgctcca gtttttaatc
    57001 tggttgtagg ccgtttcgtt aacatcgtcc tgaatcagcc gcgtcacgca gtcatcttca
    57061 taggcaatca ccggattatt gcggatgtcc gctacggtca tttccgacaa cacctgcttt
    57121 gccgccacgc gctcctgtga gcttgccgca gcaacgcccg ccagcacatc ccccgaacgc
    57181 agttcgttgg ctttagccag cacctctttt acatccttaa actgatatac attgccgaac
    57241 aatgtggtct ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg
    57301 tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc
    57361 ggacaatcac ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca
    57421 tgcccagagc tttaccaaag tcctgcccgg ccaccaccag caggggatgc ggattcggaa
    57481 aacgcgcgac gaaatcgacc aacgcgttga tgaccgtcag taccgcggcg taacgcacag
    57541 gcagcgaggc gggaagcgcc agcacgtacg cgtcagtttt aggatcaaga tccagctgaa
    57601 tcagcgcctg ttgccaggca ctcaccagat ccttttcatc aattgggatc gccaccggca
    57661 agttgcgcag cggcagttgt acgccctcca gccagattgt gctgccagag agcgaaaggg
    57721 tatgtgcacc cgcgccaatc accgtggcgc gcacggtttg cgccggaaac tgcacgttca
    57781 tctcacgcag gcgcggatgg tcatgcagcg ccgttgccag aagcgggcca atatcggcaa
    57841 aacagaacgg gtcggcgggc tggtggcgat aacattcgcc aacgccgcca gaaagcgtaa
    57901 tgatttcggg cgtaacacct acgggcagca aaccggtttg catcaatgcc tgcgcgagcg
    57961 gcgaaagcgt tccgtcaatc acttcgacaa tcagctctgc catccgccgg gtaacctgca
    58021 ccagctgcgc gccggtcagc gaacgggctt cggtgcctgc accgaagcac tcatccacaa
    58081 tcatctgccc cggtttatga gcgtaaacca cacgcccctg gctgtcggtt tccagcaggc
    58141 gaccgccgac attgaggcag gcggtgccgc tgatttttcc ggcatcgaac agggcgtagt
    58201 tcgcggtgcc accgccgata tcgatattca ggacccgaca cagccgttgt tcagaaaggg
    58261 tttgcgcccc ggcaccgtga ccggcgatca cggattcgag atgcggtcca gcgctggcga
    58321 cgacaaaatc gcccagcgac tgggagagcg tcatcaccgc cgggcgagcg ttgcgggttt
    58381 tcgcgctttc accggtgatg atgatggcac cagaatcaac gctttccggc gcaatgcccg
    58441 cagcctgata ttgctcgagg attaaggttt tcagttccgc ttcttttaaa ccaccctgtt
    58501 tatcgacagg ggtaaagaac accgggcttt gccagctaat ttcgcgtttg atgaattcgt
    58561 agcgcggcac ctgcgacacc gccgcacggt taaccagctc caggtgggag aaaatcacct
    58621 gggtggtggt ggtgccgata tcgataccga cgctcagtag ctggcgagtg ttcacgattg
    58681 cgcctccgct tcggttttag ccgcgctcgc gtcttcttta ggtaccagca tcatcgccac
    58741 gccaatcgcc gtgacgccgc cgatcagctt gccgacaatc atcgggaaga tcatggcgtt
    58801 catgttggca gccgcgaagc ctaaatggtc gcccagggcg aaagcagcgg aaacggcgaa
    58861 ggcacagttg atgactttgc cgcgggtatc catctgcttc atcatgccaa acatcgggat
    58921 gttgttggca agcgttgcca ccatgccggc tgccgctata ttgttcatat tcagcacctt
    58981 accgacactc atcagcggtt tttcaaacca gcgagtcagc agcagcacca tcggatacgc
    59041 ccccagcagc acgcaggaga tagaaccgat aacttcaatg gcgcgcatca cttcaccggg
    59101 tttgtcgcct ggagccatga agatcggatc cagaccgggg atcagttccc aaccgagcag
    59161 gaatttcact accgcagcgg caagaccgag ggtgatcaat gcaacgagga atttggcgaa
    59221 gatctggaag ccgttgatca ttttttccgg gatgaatttc agccccagcg ccaccagcac
    59281 cgcaacgata agtaccggga tcatgttcat caggatcagc gcaaaggtga attccaccgg
    59341 ctgaccgttg atctgcacac cggagtacat agcaaccaga ccaccagcaa tacagccaat
    59401 cggaatggtc acaatgcccg ccagcacgcc gagcgccaga taacgacggt cagaaggttc
    59461 gataatgccg agcgccaccg gaatggaaaa cacaatcgtt ggccccatca tcgacccgag
    59521 aattaaccca gagtatagcc acgctgctac gtcgccgccc gccagctctt tggcgaggaa
    59581 gaagccgccc atatcgcacg ccagcagtgt tccggcgaac atcgatgggt tagcgccgag
    59641 catttcgtaa accgggataa tcaccggccc gagtacgtgg gccagtaccg gtgccagcgc
    59701 ggtcataccg accatagcca ggcccaaagc gcccattgcc ataaagcctt cttcgaactg
    59761 accgccggat ccttcgatac ttttaccgaa cttaccgagg aaacgagcag aaccgccgaa
    59821 ctgcgacagg atcctgtcta cggcagctat cagcataaag aacatcatga tgtacatgat
    59881 gatttcgtta atacccatcg cctttactcc ctgttagttg ttatttattg gcggatgcgg
    59941 cgtgaacgcc ttatccgccc tacatgtgca atcccgaagg ccagataaga cgcggcaagc
    60001 gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgtgaacgcc ttatccgccc
    60061 tacatgtgca atcccgtagg ccggataaga cgcggcaagc gtcgcatccg gcatttattg
    60121 cgccgctgcg tacaggccga caatctgctc caggctggcg gtacgcgggt tactgcgcag
    60181 acaaatatct tccagcgcgg cctgcgccca tgcgccgtaa tgcgcagatg tcgcaccaac
    60241 atcgcccagt cgtttaccaa tcccaacttc cgctatcagc tcacttaccg cattaatagc
    60301 gtcacgatcg tcggattttt tagttcgcag cgcccgacca accaagctaa accgatcacg
    60361 acaaaccatc cggttaaatt ccatcaccgt tggcaacaac atggcgttcg ccagaccgtg
    60421 cggaatatgc agcgccgccc ctggctgatg cgccatcgcg tggcacaacc caagtccggc
    60481 actggaaaac gccattcccg ccatacatga agccagcaac atgctctcgc gcgcggcaag
    60541 gtcatgaccg tagcccaccg ctttcggcag cgatttgcca atcatcgcaa tggcaccaat
    60601 cgccagactg tcggtaaacg gtgtggcgtt cagcgcgctg tacgcttcaa tggcatgggt
    60661 taacgcatca atgccagtca tcgccgtgac atgcgacggc acaccttcgg tcaatgcggc
    60721 gtcgagaatc gccacatccg gcatcagcga ggcatgggct aacacctgct tgcgcccgct
    60781 caccgcgtcg ataatcaccg ttacgttggt ggtttcagag ccggttccgg cggtcgttgg
    60841 aatggcaatc aacggcaggc gcggttgcag aacgctggtt tctgacattt ctgccagcgt
    60901 gctatcgggg ttcgtcacca gcaacgccac ggctttcgcc gcatccagca ccgagccgcc
    60961 gccaaacgcg atcaccccgt cacaacctga ttcacgcaac tgcgccacgg ctgcacacac
    61021 gtcggtgatg cacggttcgc ccaccggaca tggccagagc gtcatggcga tacctttaac
    61081 ggccaggcta cgcgtcagcc cggcggtcat ccccgcctga tgcaaaaagc tgtctgccat
    61141 cacgaacaga tgtttcagcc cacgcgtttg cgcttgctgc ccacaactgc tcaccgcgcc
    61201 cggaccgcaa agcgtcaccg gtggaacgct aaatgttttc acccgctgca gattcagggt
    61261 atcgaatgcc tgaaaaagcg ccgtttgcaa ttcactttgc atagatcccc tccgttttct
    61321 ctcgcccact actggcgatc gccagcgggg tcataaacaa actgtgctgc ggtaaatgca
    61381 cctgtaacgc cgggaattgt ttgcgaaaca gttccgctac gcccggttgc atacaggaac
    61441 cgcccgccag ccataaatcc gtaatgccct gcccttcaat atgccgggcc acaatgtccg
    61501 ccattttttc gtacaccggt ttcaccgcgg gccaaatctc gtcaccgtga ccgcgtttgt
    61561 actgctctgc ctcttccagc gaaatacggc gatttccggc gagagtcaga gagatgtgat
    61621 gtccgccggt ggcttcatcc gccgagtacg tcactttgcc ctttttcacg atggcaatgc
    61681 cggtagtgcc gccgccgata tccactacac cggcgttgtc cagttgcagc agatccgcaa
    61741 ctgcggttgg ctcatccagc acatggctca cttccagtcc agcagactcc agcacgttga
    61801 tggaaatacg cgggtccgta cctggaggaa aagaggtcgc cgcatggcta aaacggcgac
    61861 cgaattgctg ctcgagggtg tcgagatggc gacgaacaat ggtgaccgca ccgaagaaat
    61921 cccagacgat gccgtcgcgg accacatcag cccagtcgag gcataccgcc accggctgac
    61981 cgtcgcggtc gacaaccatc gacaccacat cgcaggtgcc cagatccacc cccagccaca
    62041 gcggagattc cgtcgcggcg ggcgtctggt tacacagcgt tgccgccgtt tgcagacgtg
    62101 gggtgagcca ttgttcgtcg tgcgccatct gttactcctt aaacaatgcg aaacgcatcg
    62161 actaatacac agcgacgcag acggacaaac gtccgcgcgc tggttacccc ttcaccggtt
    62221 ggcgtggtga tggtcatagt ggtccagcct tccccgccca gccccagccc ggcaatgcac
    62281 ggtccgttct taacgaaaat gctggtatca atggcattcg ccatctggtt catgttctcg
    62341 atattgcgcg agtgcattgc cgccgtgtgg tggcaaccgc cttccagttt caccgccagc
    62401 gcaatggcat ccgccacgtt ggcgacgcgc acgacgggca gcaccggcat catcagttca
    62461 gtcacagcaa acggatgttc tgcggtggtt tctacaaaca gcagtcgcgt ttgttccgga
    62521 actttaaggc cgattgccgc cgcgattttc gccgcatcgc gaccaaccca gtcacggctg
    62581 acggtgcctt ttccgcgctc gtcgatattt ttcagcaaca ccgattgcag ttgctgcgcc
    62641 tgctctgcgg tcagtttcac cgcatgctgg ccttccatca gacgcatcag ttcatcggct
    62701 acgctatcaa caacaatcag taccttttcg tcggcacaaa tgatgttgtt atcgaaagaa
    62761 gcacctttga cgatggactg agctgcacgg gcgaggtcgg cggtttcatc caccactacc
    62821 ggcgggttac cggcacctgc cgcaatcagg cgtttattgg tgtgtttgcg cgccgcttcc
    62881 actaccgctt cgccgccggt caccaccagc aggccgatac ccggaaactt gaacaagcgt
    62941 tgcgcggttt cgatatccgg atttgctacg gtaaccagta agttttccgg cccgcctgcg
    63001 gcaacaatcg cctggttgag cagcgtaatc gcccgctggg agactttttt cgccgccgga
    63061 tgcggggcaa aaatgacgct gttgcccgcg gcaatcaggc tgatggcgtt attaatgacg
    63121 gttgccgccg ggttagtgga aggcgtcacc gaagccacta cgccccaggg cgcgttttca
    63181 attaaagtca ggccgttgtc gccagtcagc acctgtggag agaggcactc aacgcctggt
    63241 gtgccgcgcg cctgagcgac gtttttggca aatttatctt caacgcgccc catgccggtt
    63301 tcactgacgg caagttccgc taaatctctg gcgtgttttt cgcctgcttc acgaatggca
    63361 gcaatggcta actggcgcat tgccacgctt tttaaccctt gctgggcgac tttggctgcc
    63421 gcaacggcgt catccaggga cgcgaaaacg cccatctcat gaacggcggc gggcggcgtg
    63481 tcactgcttt gcattttcag cagtaccgct ttcaccacct gttcaatatc ctgttgattc
    63541 atgatgttct gccttatttg tggaaaatga cctgaccgcc agacaccacc tcatcgacga
    63601 tgccaatcac gcacagatcg accggtgacg tttcgctttt atgcgcctgg cgggcggaac
    63661 tgccactcac cagcaacacc cactccccgg ttcccgcgcc aatattgtcg atggcgacgg
    63721 cgcattgccc gtcaggatta ccttgtggat caatcatttc caccatcagc aatttgtcat
    63781 gcgccagtcc gtgatggcgt acggtacaaa caatttgtcc agtgacgact gccagtttca
    63841 tacccgcctc cgtggcgtat ttcaggtaaa agctccccct accctccgca gaaggtaaaa
    63901 tgaaaaagga gagagcgtga cgcccgaatc gacgtcacac agggtgatta caggttgctg
    63961 ctatcgcctt tcaggccgat cgggaagact tcttccaggt cgccgtgcgg acgcgggata
    64021 acgtgtacag ataccagctc gccgatacgc tgtgccgctg cggcacctgc atcggtcgcg
    64081 gctttacagg ctgcaacatc accacgaacc atggcagtac acaggccgcc gccaatctgc
    64141 ttaacaccaa ccagcttgac gcgcgcagct ttaaccatcg cgtcagaagc ctcaatcagt
    64201 gcaaccaggc cccgggtttc gatcattcct aatgcttcca ttgtgctttc ctctttatca
    64261 gggtccagaa cgggaccgtt cattcaacca gtgtttgtaa actgctttcg cggttcactt
    64321 ctgtctgacg ctgcacagct gccaccagcg ccagttcgat aatttcctgc acgctacaac
    64381 cacgagagag atcgtgcatc ggcgcggcaa gtccttgtat caatggcccg acggcacgat
    64441 atccgccaag tcgttgtgcg attttgtagc caatatttcc ggcttccagc gacggaaaaa
    64501 ccatcacatt agctttgccc tgtaacgggc tggcaggcgc tttttgcgcc gccacttccg
    64561 gcacgaaggc ggcgtcaaac tgcaactcgc catcgacaac cagctttggt gcgcgctcac
    64621 ggacgatttc tgtcgcctgc tggacgttgg caacgcaggg gtgacgggca ctaccgttgc
    64681 tggaaaacga caacatcgcc acgcgcggtt cttctccggt gatggcgcgc caggtttcgg
    64741 cgctggcaag cgcgatatcc gccagttgcg ccgccgtcgg ctgtggcact acgctgcaat
    64801 cggcaaagcc caatgcgggg ccactgtact gtggcagcat caggaaaatg gaggagagcg
    64861 ttttacagcc tggctgtaag ccgataatgc gtaatccggc acgcagcaca ttcgccgtgg
    64921 aagagagatt gcccgcgata cagacatccg ctgtcccggc gctgaccatt gcggcagcga
    64981 acatcagcgg atcggtgagt ttatccagcg catccggcgg cgttttttcg cccgcacggg
    65041 ccagccagcg gagagcaaat tcttcccgca ttgcgaggtt gccatgcgga tcaatcacct
    65101 gtagcccgtc catcgccacg ccgtgactga gcgcaaactg acgaagttca aacggattgg
    65161 cgaccagaat gggcgttgcc agaccttgtt gatgtaaata ttgcgccgct ttcagcacac
    65221 gttgatctaa cgcatccgga aaaactactc tggcgggcgc tcgcaacgcc agtttacgac
    65281 aacgttcaat aatcattgcg tttcccccag tcgttgttgg atctgcttca ccgttagcga
    65341 ctgcttcgtt acgctcagga tcatcatcac gtaaaccgtg ctggaaaggc ggttcagcgc
    65401 ctgcaagatg tccgggcgta atacttcaaa gctgcgggtg ataaacacct gcgccgctac
    65461 agtttcggtt tcccgcactt tggtgcgtag cagattgagc aacgccgcat cacgcccgtg
    65521 gctggcttcc ggcaccagat gatcgtggtc gagatagcgc agcggctggt gagaaagccg
    65581 atgcagatct tcgtcagaaa gcccgacaat cgcttgttcg cccagcggtt cccccagcgc
    65641 atcggcgcgc ataatgttgc caagacgcga acggatatcc gccagccacg gctgccacgg
    65701 ttccgccagt tcaatttgca gccacactgc cagcgcaatg gtgctgtcga gaacagcgcg
    65761 aaaagccagg cgcggatcgc ttttggcaac cattttttcc gccgacaggt gagtcagcgt
    65821 atccggcttt tttgccaccg gctggcggca cagttcgcag cacgcctgcg gatgttcatc
    65881 gctactggtt agcccgtgaa cgggctgcgg ctgctgctgt tcatcgtcaa caaacaggcg
    65941 gccctgctcg tcaataaact tgatgcgcag atggcggctt tccagtaact cccgggcaga
    66001 gggcgtcagg cgactgtccg cagggagatg gatctctgcg ccttcgctga gcgtatgatt
    66061 cgctcttagc catgcttcgg tgatgaaatc tttcataagg attgccagtt agccggccag
    66121 gcgacataca ggaatttcac gctggatgtc gtaccaaatt cgatgctgga gcctttcggg
    66181 ataaacatca catcgcccgc tttggcgatc atagtttcgc cttcgtggcg aacatgcagc
    66241 tccccttcca gcaccatgtc gatttcgtcg tagttcaggg tccacggaaa gaaggcgttc
    66301 tcccactgca tgaaaccagc ggccatactg ctgccgtcgt ctccggtcac gagatcagtc
    66361 aagcctacgc agtgcggttc cgcgccatcg aaacgaccaa acttgacgct gctgccatca
    66421 ataactttga tgccgccttt gccggtcacc gatttaaagc tcggctgcag tgcaccctgc
    66481 tccagcgact gtttttcctt catcactttt tccatcagct gcgcgaccag gctttcggta
    66541 aactgacctt ccggcagttg ggcgataatg gtttcacgga tgcgctggct ttcggtttta
    66601 tcggctgatg cgctggcggg aacagacgcc gttaccggga tcgattcatc acattcggtg
    66661 atggtaaaac ccaacagatc agccacttca cgagcttccg gggtaataat gctggcgcgc
    66721 agaaccacgg acattgccag ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
    66781 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
    66841 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga agcccgtctc
    66901 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
    66961 ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
    67021 gtcattcgcg ccgtggacat agatcagcat gtccacatcc tgtagcgtgg taattaatgc
    67081 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc cattatcatt
    67141 aaattccacg gcctgcgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
    67201 cgttgttttt cctgccccga cggtacccac aaacgcaatt cgtttcatga atgcctcata
    67261 ttaacttttg gtcatttcac aaagcgtata atttaataag cgtcctaaac cactgacggt
    67321 ttgtgataac gcttcttcta ctgcgcccac cgaaccataa atcaccagcg cgccgctaaa
    67381 acggtcgaga aaaccgatat gcacatcggc ggctttcaac gctaaatcac cggcgatcat
    67441 cgccgtttct ccgggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat
    67501 tttcttcgcc agttcctcgc cgggatgcgc gatgagatgc gccagcgtga cctgtttgcc
    67561 gggcacaaat tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt
    67621 cgtgtatcgt gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa
    67681 ggtgaggtag atcactaaga atggagagat atttactgt
//
LOCUS       DAZGTJ010000014        66443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000014 DAZGTJ010000000
VERSION     DAZGTJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.014"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(188..1135)
                     /gene="dusC"
                     /locus_tag="ACHK8E_001223"
     CDS             complement(188..1135)
                     /gene="dusC"
                     /locus_tag="ACHK8E_001223"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP0343518.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(1197..2390)
                     /locus_tag="ACHK8E_001224"
     CDS             complement(1197..2390)
                     /locus_tag="ACHK8E_001224"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFP0343519.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2405..3049)
                     /gene="maiA"
                     /locus_tag="ACHK8E_001225"
     CDS             complement(2405..3049)
                     /gene="maiA"
                     /locus_tag="ACHK8E_001225"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFP0343520.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3057..3758)
                     /locus_tag="ACHK8E_001226"
     CDS             complement(3057..3758)
                     /locus_tag="ACHK8E_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0343521.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3774..4802)
                     /gene="gtdA"
                     /locus_tag="ACHK8E_001227"
     CDS             complement(3774..4802)
                     /gene="gtdA"
                     /locus_tag="ACHK8E_001227"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFP0343522.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4814..6172)
                     /locus_tag="ACHK8E_001228"
     CDS             complement(4814..6172)
                     /locus_tag="ACHK8E_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343523.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            6299..7207
                     /locus_tag="ACHK8E_001229"
     CDS             6299..7207
                     /locus_tag="ACHK8E_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0343524.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7338..7736
                     /gene="yohJ"
                     /locus_tag="ACHK8E_001230"
     CDS             7338..7736
                     /gene="yohJ"
                     /locus_tag="ACHK8E_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP0343525.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFVPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7733..8428
                     /gene="yohK"
                     /locus_tag="ACHK8E_001231"
     CDS             7733..8428
                     /gene="yohK"
                     /locus_tag="ACHK8E_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP0343526.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            8558..9442
                     /gene="cdd"
                     /locus_tag="ACHK8E_001232"
     CDS             8558..9442
                     /gene="cdd"
                     /locus_tag="ACHK8E_001232"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP0343527.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9592..10311
                     /gene="sanA"
                     /locus_tag="ACHK8E_001233"
     CDS             9592..10311
                     /gene="sanA"
                     /locus_tag="ACHK8E_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP0343528.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10314..10553
                     /gene="yeiS"
                     /locus_tag="ACHK8E_001234"
     CDS             10314..10553
                     /gene="yeiS"
                     /locus_tag="ACHK8E_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP0343529.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10872..12110
                     /gene="preT"
                     /locus_tag="ACHK8E_001235"
     CDS             10872..12110
                     /gene="preT"
                     /locus_tag="ACHK8E_001235"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP0343530.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
     gene            12104..13339
                     /gene="preA"
                     /locus_tag="ACHK8E_001236"
     CDS             12104..13339
                     /gene="preA"
                     /locus_tag="ACHK8E_001236"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP0343531.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(13410..14420)
                     /gene="mglC"
                     /locus_tag="ACHK8E_001237"
     CDS             complement(13410..14420)
                     /gene="mglC"
                     /locus_tag="ACHK8E_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP0343532.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14436..15956)
                     /gene="mglA"
                     /locus_tag="ACHK8E_001238"
     CDS             complement(14436..15956)
                     /gene="mglA"
                     /locus_tag="ACHK8E_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP0343533.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16017..17015)
                     /gene="mglB"
                     /locus_tag="ACHK8E_001239"
     CDS             complement(16017..17015)
                     /gene="mglB"
                     /locus_tag="ACHK8E_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP0343534.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17295..18335)
                     /gene="galS"
                     /locus_tag="ACHK8E_001240"
     CDS             complement(17295..18335)
                     /gene="galS"
                     /locus_tag="ACHK8E_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP0343535.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(18477..19634)
                     /gene="yeiB"
                     /locus_tag="ACHK8E_001241"
     CDS             complement(18477..19634)
                     /gene="yeiB"
                     /locus_tag="ACHK8E_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP0343536.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            complement(19651..20319)
                     /gene="folE"
                     /locus_tag="ACHK8E_001242"
     CDS             complement(19651..20319)
                     /gene="folE"
                     /locus_tag="ACHK8E_001242"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP0343537.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20577..21413
                     /gene="yeiG"
                     /locus_tag="ACHK8E_001243"
     CDS             20577..21413
                     /gene="yeiG"
                     /locus_tag="ACHK8E_001243"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0343538.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21445..23424)
                     /gene="cirA"
                     /locus_tag="ACHK8E_001244"
                     /pseudo
     CDS             complement(21445..23424)
                     /gene="cirA"
                     /locus_tag="ACHK8E_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(23717..25186)
                     /gene="lysP"
                     /locus_tag="ACHK8E_001245"
     CDS             complement(23717..25186)
                     /gene="lysP"
                     /locus_tag="ACHK8E_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP0343539.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25391..26272)
                     /gene="yieE"
                     /locus_tag="ACHK8E_001246"
     CDS             complement(25391..26272)
                     /gene="yieE"
                     /locus_tag="ACHK8E_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP0343540.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26371..27420
                     /gene="yeiH"
                     /locus_tag="ACHK8E_001247"
     CDS             26371..27420
                     /gene="yeiH"
                     /locus_tag="ACHK8E_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP0343541.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27494..28351
                     /gene="nfo"
                     /locus_tag="ACHK8E_001248"
     CDS             27494..28351
                     /gene="nfo"
                     /locus_tag="ACHK8E_001248"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP0343542.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28354..29442
                     /gene="yeiI"
                     /locus_tag="ACHK8E_001249"
     CDS             28354..29442
                     /gene="yeiI"
                     /locus_tag="ACHK8E_001249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0343543.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29498..30748)
                     /gene="nupX"
                     /locus_tag="ACHK8E_001250"
     CDS             complement(29498..30748)
                     /gene="nupX"
                     /locus_tag="ACHK8E_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0343544.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30848..31789)
                     /gene="rihB"
                     /locus_tag="ACHK8E_001251"
     CDS             complement(30848..31789)
                     /gene="rihB"
                     /locus_tag="ACHK8E_001251"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP0343545.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            31919..32617
                     /gene="yeiL"
                     /locus_tag="ACHK8E_001252"
     CDS             31919..32617
                     /gene="yeiL"
                     /locus_tag="ACHK8E_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP0343546.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32685..33935)
                     /gene="psuT"
                     /locus_tag="ACHK8E_001253"
     CDS             complement(32685..33935)
                     /gene="psuT"
                     /locus_tag="ACHK8E_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0343547.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34029..34967)
                     /gene="psuG"
                     /locus_tag="ACHK8E_001254"
     CDS             complement(34029..34967)
                     /gene="psuG"
                     /locus_tag="ACHK8E_001254"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP0343548.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(34955..35896)
                     /gene="psuK"
                     /locus_tag="ACHK8E_001255"
     CDS             complement(34955..35896)
                     /gene="psuK"
                     /locus_tag="ACHK8E_001255"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP0343549.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36320..38011)
                     /gene="fruA"
                     /locus_tag="ACHK8E_001256"
     CDS             complement(36320..38011)
                     /gene="fruA"
                     /locus_tag="ACHK8E_001256"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP0343550.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38028..38966)
                     /gene="fruK"
                     /locus_tag="ACHK8E_001257"
     CDS             complement(38028..38966)
                     /gene="fruK"
                     /locus_tag="ACHK8E_001257"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP0343551.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(38966..40096)
                     /gene="fruB"
                     /locus_tag="ACHK8E_001258"
     CDS             complement(38966..40096)
                     /gene="fruB"
                     /locus_tag="ACHK8E_001258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP0343552.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40464..41645
                     /gene="setB"
                     /locus_tag="ACHK8E_001259"
     CDS             40464..41645
                     /gene="setB"
                     /locus_tag="ACHK8E_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP0343553.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41642..41896)
                     /gene="yeiW"
                     /locus_tag="ACHK8E_001260"
     CDS             complement(41642..41896)
                     /gene="yeiW"
                     /locus_tag="ACHK8E_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0343554.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42051..42623
                     /gene="yeiP"
                     /locus_tag="ACHK8E_001261"
     CDS             42051..42623
                     /gene="yeiP"
                     /locus_tag="ACHK8E_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP0343555.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42846..44312
                     /gene="yeiQ"
                     /locus_tag="ACHK8E_001262"
                     /pseudo
     CDS             42846..44312
                     /gene="yeiQ"
                     /locus_tag="ACHK8E_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            44430..45416
                     /gene="yeiR"
                     /locus_tag="ACHK8E_001263"
     CDS             44430..45416
                     /gene="yeiR"
                     /locus_tag="ACHK8E_001263"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP0343556.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45455..46168
                     /gene="lpxT"
                     /locus_tag="ACHK8E_001264"
     CDS             45455..46168
                     /gene="lpxT"
                     /locus_tag="ACHK8E_001264"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP0343557.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46580..47146
                     /gene="mepS"
                     /locus_tag="ACHK8E_001265"
     CDS             46580..47146
                     /gene="mepS"
                     /locus_tag="ACHK8E_001265"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP0343558.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47327..48883
                     /gene="pdeN"
                     /locus_tag="ACHK8E_001266"
     CDS             47327..48883
                     /gene="pdeN"
                     /locus_tag="ACHK8E_001266"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0343559.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            48965..50779
                     /gene="yejA"
                     /locus_tag="ACHK8E_001267"
     CDS             48965..50779
                     /gene="yejA"
                     /locus_tag="ACHK8E_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP0343560.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            50780..51874
                     /gene="yejB"
                     /locus_tag="ACHK8E_001268"
     CDS             50780..51874
                     /gene="yejB"
                     /locus_tag="ACHK8E_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP0343561.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51874..52899
                     /gene="yejE"
                     /locus_tag="ACHK8E_001269"
     CDS             51874..52899
                     /gene="yejE"
                     /locus_tag="ACHK8E_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0343562.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52901..54490
                     /gene="yejF"
                     /locus_tag="ACHK8E_001270"
     CDS             52901..54490
                     /gene="yejF"
                     /locus_tag="ACHK8E_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP0343563.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(54494..54838)
                     /gene="yejG"
                     /locus_tag="ACHK8E_001271"
     CDS             complement(54494..54838)
                     /gene="yejG"
                     /locus_tag="ACHK8E_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP0343564.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55172..56362)
                     /gene="bcr"
                     /locus_tag="ACHK8E_001272"
     CDS             complement(55172..56362)
                     /gene="bcr"
                     /locus_tag="ACHK8E_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP0343565.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56390..57085)
                     /gene="rsuA"
                     /locus_tag="ACHK8E_001273"
     CDS             complement(56390..57085)
                     /gene="rsuA"
                     /locus_tag="ACHK8E_001273"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP0343566.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57235..58995
                     /gene="radD"
                     /locus_tag="ACHK8E_001274"
     CDS             57235..58995
                     /gene="radD"
                     /locus_tag="ACHK8E_001274"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0343567.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59120..59404
                     /gene="rplY"
                     /locus_tag="ACHK8E_001275"
     CDS             59120..59404
                     /gene="rplY"
                     /locus_tag="ACHK8E_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP0343568.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(59543..60550)
                     /gene="yejK"
                     /locus_tag="ACHK8E_001276"
     CDS             complement(59543..60550)
                     /gene="yejK"
                     /locus_tag="ACHK8E_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP0343569.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60732..60959
                     /gene="yejL"
                     /locus_tag="ACHK8E_001277"
     CDS             60732..60959
                     /gene="yejL"
                     /locus_tag="ACHK8E_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP0343570.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            60979..62739
                     /gene="yejM"
                     /locus_tag="ACHK8E_001278"
     CDS             60979..62739
                     /gene="yejM"
                     /locus_tag="ACHK8E_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP0343571.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62814..62890
                     /locus_tag="ACHK8E_001279"
     tRNA            62814..62890
                     /locus_tag="ACHK8E_001279"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62848..62850,aa:Pro,seq:ggg)
     gene            complement(63016..63775)
                     /locus_tag="ACHK8E_001280"
                     /pseudo
     CDS             complement(63016..63775)
                     /locus_tag="ACHK8E_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            64095..64742
                     /gene="narP"
                     /locus_tag="ACHK8E_001281"
     CDS             64095..64742
                     /gene="narP"
                     /locus_tag="ACHK8E_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP0343572.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(64777..65829)
                     /gene="ccmH"
                     /locus_tag="ACHK8E_001282"
     CDS             complement(64777..65829)
                     /gene="ccmH"
                     /locus_tag="ACHK8E_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP0343573.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(65826..66383)
                     /gene="dsbE"
                     /locus_tag="ACHK8E_001283"
     CDS             complement(65826..66383)
                     /gene="dsbE"
                     /locus_tag="ACHK8E_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP0343574.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT        16196 a        17501 c        16500 g        16246 t
ORIGIN      
        1 cggcttatcc cttacggcta ccgggtcggg cgcgaggtaa catccctgta aaacgcgccc
       61 tcagcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggcg attttgacgc
      121 caccaaacaa ccgtgcggcc tattgataaa gagctaacac attgtcaaaa aacatcacta
      181 tggtttctta gagtttctcg atatcaattg cctgaatagc ccttgcaata tcaggggaat
      241 tattcaacac ccgaacatgc tgaaataatt ccgttgcttc atcgtattct ttacgcaaat
      301 aactcaacca ctgtttaatc cgcgcaacgt gatataaccc ggtatcgccc tgcttttcca
      361 gacgggtata tttttgcagc aaagcaacta cctccggcca cggcattcgc ggttcgttat
      421 attttaccac ccggctcagg ttgggaatat tgagcgcccc gcgaccaatc atcactgcgt
      481 cgcatccgct gatcgtcatg cattgttgcg cactctgcca gtcccagatt tcaccgttgg
      541 caatcacagg aatattcaac cgctggcgaa tctcgccaat cgcctgccag tcaatatgct
      601 ccgcgcggta accctgctct tttgtccgcc catgtactac cagctccgta gcgccagcct
      661 gttgcaccgc atcggcgatt tcaaatttct tctcaccgct gtcccagccc agacgcactt
      721 ttacgctgac aggcaaatgc gccggtacag cttcgcgcat cgcttttgca ccctgataga
      781 tgagttcagg atctttgagt aacgtcgccc caccgccgct accgttaacc gttttcgacg
      841 ggcagccgca attgagatcc acgccccagg aacctaactc gaccgcacgg gcggcattct
      901 ctgccagcca ttgtgggaac tgacctaaca actgcacgcg caccagcgta ccggctggtg
      961 tccggctggc gttttgtagc tcagggcaaa tgcgatgaaa gacttttacc ggcagcagtt
     1021 gatccaccac gcggacaaac tcggtgatgc acagatcgta gtcgttaact tcggtcagca
     1081 attcacgcac cagagagtcg agtacaccct ccatcggtgc cagtaacaca cgcatatcat
     1141 cacccgcaaa aaaatgaggc gctatgttag cgcctatggt cagcggatta aagtactcag
     1201 cgtggttgca aacagttatc ttctttccag ccgtacagcc actccatgcc gcggtaaaac
     1261 tctgcctgcg atttcccttt ccacaacagg ttacgtacct ggcgctctac tcctgcggca
     1321 tgataaaccc gccccatttc acgggtcgac cagacgatgc gcgcggtgcg cggaatacgt
     1381 accgactcat acagcgcaaa ggcttgtgcc gcatcaccct cacactgcgc cagcgcctta
     1441 cccagtgtta ccgcgtcttc cagcgccata caggcaccct gcgccatata ctgcgccacc
     1501 ggatgggccg catcgcccac cagcgtaatg cgcttcgttc cccacttcgc taccggttca
     1561 cggtcggcgg ttgaccagcg tcgccaggag gtcggtttat ccagcatctg gcgcggacgg
     1621 gggtgaatcc cttcaaagta agagagcact tcctctttac tgccatcctt cacgccccac
     1681 tcttcctgct ggcggctgtg gaatgtcacc accaggttat actgcttgcc gccgcgtaaa
     1741 gggtaatgca ccaagtgaca gcacggcccc gcccacagta ctggtgcgtt aatgcgcaaa
     1801 tcttccggca tatcggcaca atcgataaca gcacggtaga ccacatgccc ggtgacacgc
     1861 ggcgcatcgc ccaataagct ctggcgcacc actgacttca cgccgtcaca gccaatcaga
     1921 atatcgccga tccaactatt gccgcgatcg tcgaaaacgg tgacatcgtc ttctgtctcg
     1981 cgaatatcca cgacgttggt ggaggtgtga tattccacgt ccggatgcgt cagtaccgct
     2041 tcccacaccg aggcgtgaat atcaacccgg tggatcaccg cgtaagggcc gccgaaatga
     2101 tcgcgaaagg cctgaccggt ttcaatgcgc accacttctt cggcgtttac cgcatccatc
     2161 atggtgatgt gatcggtgaa taccgcgcgc tggcgggcga tttcgccgac gccgaggcta
     2221 tccaacgccg aaaacgcatt tggccccaac tggatacctg cgccaatctc accgatctca
     2281 tgcgcctttt ccagcagcac cactttgatc ccctggcggg ccagtgacaa tgcagttgcc
     2341 gcaccgccga tcccgcctcc cacaattact gcgcgtgtca ctttagtcat tatggttctc
     2401 ctggtcaggc tgaaatttta tcttgttgat tttctggcgc tgcggcaatg aacgctggca
     2461 gctgcgtgca ggcgtcgtat accgctttgc agcgcggata cccgctcaga tcgcagttca
     2521 tacgtagcgc gttcgcccac tgtggaacaa ggcagcaatc tgccagcgtc ggcgtctcgc
     2581 ccacacaaaa ttgccccgac tggctctggc gcagtagctg ttccaccgcg cttaaccctt
     2641 gctggatcca gtgcgcatac cagcgttttt tatcttcttc actgacgttc agctcttcgg
     2701 tcaggtagcg cagcacgcgt aggttattca atggatgaat atcgcaggcg atggcataga
     2761 caatttccag caccttgttg cgggccggtt cttcctgcgg catcagcgga acttgcgggt
     2821 agtgactctc cagccagtca ataatcgcca gcgattgtcc gagtgattcg ccctcgtcgg
     2881 tcagcagcgt cggcaccagc ccgaccggat tcatccgccg gtaggccagt tcgttttgct
     2941 gaccaatgcg gatattcacc ccttcggtct ggtaatcgat acccttcagc gccagcgcaa
     3001 tgcgcacccg gtaagacgct gaactattaa aaaaactgta cagcttcatc atcacctcag
     3061 acaatcttca ccgcgatagg cgttaagccc tcgacgttgc ccgtgatcac atcacctttg
     3121 actactgagc caaccccttc cggcgtaccg gtaaagatta gatcgcccgg ttgcagttca
     3181 aagaagcctg acaggtagct gatggtttca ttcactgacc agatgagatg acgaatatcg
     3241 ctgcgctgat gatcttcgcc gttaacctgt aaccagatag gtgcgttatc aataccgcca
     3301 gtttcgctgg ctttgtgcag cggcgcgatg ggggccgaga gatcgaacgc tttgccgatt
     3361 tcccacgggc ggcccatctg gcgcatttcc atttgacggt cacggcgagt catatcaaga
     3421 cccgtggcat aaccccagac atattcatgg gcgttttcca gcgggatatc actgcctttt
     3481 ttaccaatcg ccaccaccag ttcaatttca taatggtagt tatcggtctg cgccggatac
     3541 ggcagcgcca gcgtttcgcc agcggcaacc ggaacaatgg catccgccgg tttgcagaag
     3601 aagaatggcg gttcacgatc cggatcaaag cccatttcgc gggcgtgggc ggcatagtta
     3661 cgtccgacgc agtaaacgcg gcgtacgggg aattgttcat cactgccaac aaccggtacg
     3721 gtaacgggcg cttgtggttc gaatacgtat tgagtcattt tctctctccc aaattaataa
     3781 cgtgcttcgc ggaacagccc cagggcttct tgtaccggtc tgtccgaaaa actaaataaa
     3841 acggtatctt gcgtggtctg gaacgacacg ccgtgccagg tcggcaccac gaagatatct
     3901 tttgcactaa agctgaaggt ttcattgccg atagtgacct gcccgctacc ttccaccaca
     3961 tggtagatgg tgctgtcagt ggtacgcgcc acgcgtgagg caaacccttt cggcagcagt
     4021 tgcagaaaag cgcccatcga cggcatcgga tagccaccgg tgaccgggtt gacgtagcgc
     4081 attttgtagc catcccactc atcggcatcg cccaggcggg ttaaatcgtg tagcacttcg
     4141 cggctacggt cataacggta gttaaagatg ggcgaggagt tcccggtctg atggcgcaac
     4201 ggcaacatat tggcagcgta acgcggcagg taatcgccct ctttacgcgt tactggttgt
     4261 tgctcctccg ggtaatcttc ggcaaaaccg cagcccagag tgttcactaa tggcagatcc
     4321 agcccatcga gccagataac cggttcgtcg ccagggttac cgtgatcgtg ccagcgccac
     4381 tgcggggtca ggataaaatc gccctcattc attgacgtgc gttcgccatc aacggcggtg
     4441 aatgcccctt taccttcaac aataaaacgc agcgccgact ggttatggcg atgactcggt
     4501 gccacttcgc ccggcatgat cagttgcaaa ccggcatata aagtcgcggt gatggaagac
     4561 tgcccgcgca gcgccgggtt ttccagcacc agcacgcggc gcacggcttc tttcgcgcca
     4621 atcaggctgc cgctctccag cagcaacggg cggatctcct gataattcca gtacgctggc
     4681 gcacagttag cgttgggcgt ttttggcacc agatggtgca gtgactccca cagcggggtc
     4741 aggttttgcc cggaaatatg ctgataaaat tgctcgcggc tattttgatt gttatcagtc
     4801 attgggatgc tccttattcg ttcacaacac ctgcgtgagg cagtgagtca gggacagagg
     4861 tcgactggcg aaccacggtg atcagctggg ttaacatcac cgcactgatc gccgcgggca
     4921 cagcgataat gaaaaacagg gtgtcgaaag agaaattcat cgccatcata acgccgccag
     4981 acagcgagcc aacaatcgcg ccgcagcggc ccacggcgtt tgaccagctc acgcccgtgg
     5041 cgcgactttg tgttggataa agtgttgcgg tcagcgcatt cagccccacc tgcgatccgc
     5101 tgatgccaat gccggtgccg aaaatcgcca gcgccattag ccataatccg tcctggctta
     5161 agccaatcat cacgatgcat acagcgccta tggcatagct cagcgccagt acgcggaaag
     5221 gattgaactt atccataagc acgcccagcg ccagcgcgcc caacgtaccg ccaatctgga
     5281 acgcggcggt aacccaggag gcatgttgca ggtcgatgcc gcgatggttg agcagcgttg
     5341 gcatccagct tgagagcagg taaataatca gcaagctcat aaagaacacc acccacagca
     5401 tcaacgtgat gggtaactga cgccccataa acagttgacg aatgctgcct tttgtaacgg
     5461 aggctgattc aatgagataa aagtgggtat cgacataacg ctcgcggatg atggcgctga
     5521 cggttttcgc aatcaccgcc tggggtagct ggcggcgcac ctgccagcgt ggcgattccg
     5581 gcagaaccac cagcagtgca acaaacagca tcagcggtaa tacgccaccc aacaccagga
     5641 tcccgtgcca gccaatcacc ggcaccagct gcgcgctgac aatcccgcca aacgccgaac
     5701 ccagggtaaa gccgcaaaac atcagcgtca ccagcgcgcc gcggcggcgg gcaggcagat
     5761 attccgaggt catggtaatt gtatttggca tcgcgccacc cagcccaagg ccggtgagga
     5821 aacgcaggaa taccagcgtt tgcagatccg gtgagaaagc cgaggcgagg cttaacgcgc
     5881 caaacaaaaa gacgcacagc tcaatcaccc gtttgcggcc aaagcgatcg gacaacggac
     5941 cacaaagcag tgcgcctgcg gttaacccca gcagacccgc gccaaaaagc ggggcgagat
     6001 cgcctgcggt gagttgccag tgagtgcgaa tatcaggggc gataaagccg atagccgcgg
     6061 tgtcaaaacc atcgagcatt accaccagaa aacagcagat gataacgcgc cactgcatct
     6121 tgccgaccgg agcagcatcg atcagtgctt gtaaatcacg tcgttgagtc atagtaaatg
     6181 cctcagtgca ggtaaggtag atgtttcatt tgttttatgt atgttgcgat attgtgatta
     6241 aagagatgaa atgtacaatg gcatttcgga cagagagata acctggaggt tatggcaaat
     6301 ggcaaactgg gcgcagaaac taaagcttca tcacctgcaa atgctggtgg cgctgggtga
     6361 acagggcaat ctgacccatg tcgcgcggat gatgaatatc acccagcctg cgctgtcgaa
     6421 atggttgtca caactcgaag atgagatcgg cctcacactc tttgagcggc acagcaaagg
     6481 acttcgtcct actgaaggcg gcaaattgtt attgcaacat gcccaacggt taatcaatga
     6541 catggagcgc tcgcaacatg aactggcccg ttttaaacaa ggtggactgg tcggcagtct
     6601 gaaaattggc tgttctcccg tcgccactga ctgcgtatcc caggcgattt tgcatttgct
     6661 caaggagatg ccaactctac acctgaatat cgaagagaag gtgatgaccc cattgctgca
     6721 agatctgttg gcaggttctg tcgatgtggt ggttgggcga attggcggtc gcgcgttgca
     6781 gctgccgctg aattatcgcg tgctctacac cgagccggtg tgttttgtcg cccgtcctga
     6841 gcatccactg gcgaaatacg ccaccctcag ctggaatgat cttgctaact ggcggtggat
     6901 agtctggccg acaggtaccc ccatccgcat cagcattgat aatgcgctgg tagaccacgg
     6961 cgtgatgctg ccagaaaaca cgattgagtc ggcgtcgatg aacgtcagta ccaacctgtt
     7021 acaaagcagc gatatgatct ctattttgtc attacgcctg gcgcaacgtt atgccagcca
     7081 gggacaactg gtgattctca acctgccaaa gatagaacag aaaggcagcg tcggtgtgtt
     7141 ctggcgcaat aacgaaacgc cttcttatgc tttaagtcag tttctgcact ttttacagca
     7201 gaattagtgt gctgaactgg gcgctcgccc gaggttacgt ccctaaaatt caactatgct
     7261 gagatttctt aatgtgatcg gtagcacgtt ttacgaatta attgtatgat gaatccatct
     7321 catctggggt gttgattatg agtaagacac tgaacattat ctggcaatat ttacgtgctt
     7381 ttgtcctgat ttatgcctgc ctgtatgcag gcattttcat tgcttccctg ctaccggtaa
     7441 ccattccggg cagcatcatc gggatgctga tcctgtttgt cctgctggcc ttgcaaattc
     7501 ttccggcgaa atgggtcaat ccggggtgct acgtactgat tcgctatatg gcgctattgt
     7561 ttgtgccgat tggtgtaggc gtcatgcaat attttgattt gctccgcgca cagtttgttc
     7621 cggtagtggt ttcctgtgca gtcagtacgc tggtggtttt tctggtggtg agctggagtt
     7681 cacaactggt gcacggtgaa cgtaaagtcg taggtcagaa aggatcagaa gaatgatggc
     7741 gaatatctgg tggtcattac cgctgacgtt gattgtcttt tttgccgccc gcaaactggc
     7801 ggcacggtat aagtttcccc tgcttaaccc gttgctggta gcaatggtgg tgatcattcc
     7861 ttttttaatg ctgaccggca tctcttacga cagctacttt aaaggtagcg aagtgctaaa
     7921 cgatctgctt caaccggcgg ttgtcgcact ggcctatcct ttatatgagc agctgcacca
     7981 gatccgcgcg cgctggaaat cgatcatcac catctgtttt atcggcagcg tggttgcgat
     8041 ggtgacgggg acttctgtgg cattgttgat gggcgcttca ccggaaatcg ccgcgtcaat
     8101 cctgccgaaa tcagtcacca cgcctattgc aatggcggtt ggcggcagta ttggcggtat
     8161 tccggcaatc agcgcagttt gcgtgatttt cgtcggcatc ctcggcgctg tgtttggcca
     8221 tacattgctt aatgcgatgc gtattcgtac caaagctgcg cgcggtctgg caatgggcac
     8281 agcctcgcac gccctcggta cggcgcgctg cgccgagctg gattatcagg aaggtgcatt
     8341 tagttcgctg gcgctggtgt tatgcgggat tattacttcg ctgatcgcac cgttcctttt
     8401 cccgattatt ctggcagtaa tgggctaaaa tttgcgatgc gtcgcgcatt tttgatgtaa
     8461 gtttcacgcg ttgcataatt aatgagattc agatcacata taaagccata acgggttcgt
     8521 aaactgttat cccattacat gattatgagg caacgccatg catccacgtt ttcaaaccgc
     8581 ttttgcccaa cttgcggata acttgcaatc tgcactggaa cctattctgg cagacaagta
     8641 cttccccgct ttgttgaccg gggagcaagt ctcatcgctg aagagcgcaa cggggctgga
     8701 cgaagacgcg ctggcattcg cactacttcc gctggcggca gcctgtgcgc gtacgccatt
     8761 gtcgaatttt aatgttggcg caattgcgcg cggtgtgagc ggaacctggt attttggtgc
     8821 caatatggaa tttattggtg cgacaatgca gcaaaccgtt catgccgaac aaagcgcgat
     8881 cagccacgcc tggttgagtg gtgaaaaagc gcttgtagcc atcaccgtta actatacgcc
     8941 ttgtggtcac tgccgtcagt ttatgaatga actgaacagc ggtctggatc tgcgtattca
     9001 tctgccgggc cgcgaggcac acgcgctgcg tgactatctg ccggatgcct ttgggccgaa
     9061 agatctggag attaaaacgc tgctgatgga cgagcaagat cacggctatg cgctgacggg
     9121 tgatgcgctt tctcaggcag cgattgcggc ggcaaaccgt tcgcacatgc cttacagtaa
     9181 gtcgccaagc ggtgtcgcgc tggaatgtaa agacggtcgt attttcagcg gcagctacgc
     9241 tgaaaacgcc gcattcaacc cgactctgcc accgttgcag ggggcgttaa ttctgttgaa
     9301 tctcaagggt tatgattacc cggatatcca gcgcgcggtt ctggcagaaa aagccgatgc
     9361 gccgttgatt cagtgggatg ccacctccgc aacactgaaa gctctcggct gtcacaatat
     9421 cgaccgcgtg cttctcgctt aagcctggtg ccggatgcgg cgtgaacgcc ttgtccggct
     9481 tgccagccct ctcctggtgt cgaaaatccc ggcaaacagt ttgccgtttc ttgcgcaaaa
     9541 ccagcgggta aagtagcctg atggaaattt tccttagatc gagtctcctg catgttaaag
     9601 cgcgtgttcc tcagcctgtt agtcctgatc ggcttgctgc tgttgactgt gctcggcctc
     9661 gatcgctgga tgagctggaa aaccgcgcct tatatctacg acgaattgca ggatctcccc
     9721 taccgccagg tcggtgtggt gctcggaaca gcaaaatatt atcgtactgg cgtaattaat
     9781 cagtattatc gctaccgcat tcaaggagcg attaatgcct ataacagcgg taaggtaaat
     9841 tatctattac tgagcggcga taacgcattg caaagttata atgagccgat gaccatgcgc
     9901 aaagatttaa tcgctgctgg tgtcgaccca tcagatattg ttctcgatta cgcaggcttt
     9961 cgtacgctgg attccatcgt gcgtacacgc aaagttttcg atactaatga tttcattatt
    10021 atcacccaac gtttccactg tgagcgggca ttatttattg cgctgcatat ggggattcag
    10081 gctcagtgtt atgccgtacc gtcaccgaaa gatatgctgt cagtacgtat tcgcgaattt
    10141 gccgcccgtt tcggtgcgct ggctgacctt tatattttta aacgtgaacc gcgtttttta
    10201 gggccgctgg tccctattcc ggctatgcac caggtgcctg aagatgcgca ggggtatccc
    10261 gccgtcacac ccgaacagtt acttgaatta caaaagaaac aaggaaagta gttatggatg
    10321 tacagcagtt ttttgtcgtt gccgtttttt tccttatccc ggtattttgt ttccgcgaag
    10381 catggaaagg ctggcgcgca ggcgcgattg ataaacgggt taaaaatgca ccggaaccgg
    10441 tgtatgtctg gcgagcaaaa aatcccggac tcttcttcgc ttatatggtg gcatatatcg
    10501 gcttcggaat tttatctatc ggcatgattg tttatcttat tttctatcgt taatattttc
    10561 tcttttctgt taattattga gaataattat tacttcactc aataagctgc ggatataaca
    10621 ttctgaaccg cagctatttg tgaatccttt cacagtttaa atttccccac gcgcatagcc
    10681 ttaatatcag cacgcaatat ttatactcta aataattcga gttgcaggaa ggcgacaagt
    10741 gagtgagtcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
    10801 acatgcaact tgaagtatga cgagtatatt tagcgttcgc cttaattact catagcatta
    10861 aggaagatca catgccgcaa caaaattatc tggatgaact cactccggct tttacaccgt
    10921 tactggcgat taaagaagcc tctcgctgtt tattatgcca tgacgccccc tgtagtcagg
    10981 cttgcccggc acagaccgat ccggggaaat ttattcgctc aatctacttt cgtaatttta
    11041 aaggcgctgc cgagacaatt cgtgaaaata atgccctcgg tgccgtttgt gccagagtgt
    11101 gcccgacgga gaaattatgc caaagcggtt gtacccgtgc cggtgttgat actcccattg
    11161 atatcggtcg cttacagcgt tttgttactg attttgaaca acaaactggg atggaaattt
    11221 atcagcccgg tactaaaacg ctcggcaaag tcgccattat tggcgcgggt cctgccggat
    11281 tgcaggccag tgtgacactg acaaaccagg gttatgacgt cacgatctat gagaaagaag
    11341 cacaacccgg tggctggttg cgtaacggca ttccgcaatt ccggttaccg caatcagtac
    11401 tggatgcaga aatcgcccgt attgaaaaaa tgggcgtgac cattaaatgc aataacgaaa
    11461 tcggtaacac actcaccctg gagcagctga aagcggaaaa ccgcgcggta ctggtcactg
    11521 tggggttatc gagcggttcg gggctaccac tgtttgagca tagcgatgtt gagattgccg
    11581 tcgacttctt gcaacgtgca cgacaggcgc aaggcgatat cagcattcca caaagcgcat
    11641 taattatcgg cggcggtgat gtcgcgatgg acgtagccag cacgctgaaa gttctcggct
    11701 gtcaggcagt aacttgcgta gcgcgtgaag agttagatga gtttccggca agcgaaaaag
    11761 agtttgccag cgcccgggag ctgggcgttt cgatcatcga tggattcacg ccagtagctg
    11821 tggaaggcaa taaagtcacg tttaagcatg tacggttacc gggcgaactg acgatggcgg
    11881 cagataaaat tattctcgcc gtcggtcagc atgccagact ggatgccttt gcggagttag
    11941 agccgcagcg taacaccatc aaaacacaaa attaccagac ccgcgacccg caagtctttg
    12001 ctgctggcga tattgttgag ggtgacaaaa ccgtggttta tgccgtgaaa accgggaaag
    12061 aagccgctga ggcgattcat cactatttaa agggagcttg ctcatgttaa cgaaagatct
    12121 ttcgattacc ttttgtggtg tgaagtttcc caacccgttc tgcctctctt cttcgcctgt
    12181 aggcaactgc tatgagatgt gtgccaaagc ctacgacaca ggctggggtg gcgtggtgtt
    12241 taaaacgatc ggttttttta tcgccaacga agtctcgccg cgttttgatc atctggtgaa
    12301 agaagatacc ggttttatcg gcttcaaaaa tatggagcag attgctgaac atccgttgga
    12361 agataatctg gccgcactgc gtcggctgaa agaagattac ccggacaaag tattgatcgc
    12421 ttcaatcatg ggcgaaaatg agcagcaatg ggaagagctg gcgcgtctgg tgcaagaagc
    12481 tggcgcggat atgatcgagt gtaacttctc ctgtccgcaa atgacttctc atgcgatggg
    12541 tagcgatgtc gggcaaagcc cggagctggt agaaaaatat tgtcgggcag taaaacgtgg
    12601 ttccacgctg ccaatgctgg cgaagatgac gccgaatatc ggtgatatgt gcgaagtggc
    12661 gctggcggcg aagcgcggcg gcgcagatgg cattgcggcg attaacaccg ttaaatccat
    12721 caccaatatc gatcttaatc agaaaatcgg tatgccgatc gttaacggta aatcgagtat
    12781 ttccggatat tccggtaaag cggtaaaacc gatcgccctg cgcttcattc agcaaatgcg
    12841 tacccatcca gaactgcgcg atttcccgat cagcggtatc ggcggcattg aaacctggga
    12901 agatgcggcg gagtttttat tgctcggtgc agcgacgttg caggtgacca ccggcattat
    12961 gcagtacggg tatcggatag tggaagatat ggcaagcggg ttgtcgcatt atctcgccga
    13021 tcaagggttt gattcgttgc aggagatggt aggtctggcg aataacaata ttgtcccggc
    13081 ggaagattta gaccgcagtt atattgtcta tccccatatc aatctcgata aatgtgttgg
    13141 ctgtggacgc tgttatattt cctgttacga cggcggtcac caggcgatgg aatggagcga
    13201 gaaaacccgt acaccgcatt gtaataccga gaaatgtgtt ggttgcctgc tttgtggtca
    13261 cgtctgcccg gtgggttgta ttgatctcgg agaagtgaag tttaagaagg gagagaaaga
    13321 acacccggta acgttgtaaa accagagacg catccggcat ttggcaccga ttgccggatg
    13381 cggcgcatcc ggcaataccc acattcacat catttcttac gcgcgtattt cagtgaatcc
    13441 agcgccacgg cgaagataat aatcgcccct ttgatgatgt actgccagta tgggtttacg
    13501 ccgatgtacg tcaggccata gttgatgacg gtaaaaataa tcaccccggt caccacgcca
    13561 atcaccgtcc ctacaccacc gctgaacgat acaccaccca ccacacatgc cgcgatagca
    13621 tccaactcat acataaagcc gaggttgttg gtggcagagc cgatacgtcc ggcttctaac
    13681 atcccgccga aggcatagaa cacgccagac aacgcgtaga tcatcagcag gttcaggccg
    13741 acgttgacac cagatacttt cgccgcttcc gggttaccgc caatggcaaa aatgttctta
    13801 ccgaagcggg ttttgttcca caacacccag acgaacgcca ccgcaatcaa cgcgtagaag
    13861 gtgatgtaag agagacggaa actccccagc gcgacaaagc cctgagcaaa ggtagagaag
    13921 ccactgtcga aaccagaaat cggcgacgcc ccgacaaagt cgtaatagag cgagttgatg
    13981 ccatagacga tgatcatcgt gccgagcgtg gtaatgaacg gcgtcacgtt gagataggcg
    14041 ataatcagac cgttgatcaa accgatcacc gcaccaatgg cacagacaat cagaataacc
    14101 agcgcaatcg gcatcgtcgc catttccggg aacactttgt tggcgttatc catggactgc
    14161 aataacgtcg ccgctactac tgccgccagc cccacctggc gacctgcaga aaggtcggtc
    14221 ccctgggtga caattaaccc tgccacaccg agcgcgataa taatacgcac cgatgactgg
    14281 gtgagaatat tacttaagtt caacagactt aaaaatgttg ggtcctggaa aataataatt
    14341 gccagcaaaa ctaaaagaac gacgtaaata ccgccctctt tcaggtaagt aagaaaactt
    14401 ttcttattta acgcactcat gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc
    14461 agaatttcgt tttgcgttgt tgtttttgta tcgacaattc cggaaacgag accattgctc
    14521 atgaccagaa tacggtctgt tatccctaac aactcaggca tttcagagga gataataata
    14581 atccctttgc ctttcttcgc cagttcggca attaactgat agatctcaaa cttcgctccg
    14641 acatcaatac cgcgcgtcgg ttcatcgagc attaatattt ctggttgcgt tagtagccag
    14701 cgaccaataa tcaccttttg ctgattaccg ccggagagcg aaccaatttg cgtccgatgc
    14761 cccggtgttt ttacccgcat cgaatcaatc acccactggg tatcgctttt catccgcgag
    14821 ttatccagta aaccaacttt atttttgtag ttgcgaatat tggaaattaa cgagttaaaa
    14881 ccgatatcca gataggcata aattcccgtt gagcggcgct cttcagttac cagtgcaaat
    14941 ccgtggttta tggcttcgtt ggcattatgg ttattgatct ttttgccgtg caacgtaatg
    15001 gtgccagccg atttctcgcg aataccaaat aacgtctcaa caatatcggt acgtttcgct
    15061 cccaccagac cggctatacc gagaatctcc cctttatgca gatcaaacga gacatcgcga
    15121 atcgacggct ggcgcagcga cgtcaggtta cgcacttcaa gaatcacttc gcccggcttg
    15181 ttttctttgt cagggaaacg ctggttaaga gaacgcccaa ccatcatggc gatgatcttg
    15241 tccatcgtca gtcctgccag cggttcagtg gcgatccact gaccgtcgcg caatacggta
    15301 acttcatcac ataactggaa gatttcttcc attttatgtg agatataaac aataccgcag
    15361 ccgcgctctt ttaatttacg aataatagtg aacagatgat tgacctcttt ttcggttaac
    15421 gaagaagtcg gttcatccat aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt
    15481 tcgatcatct gcatttgcga aacggataat gtgccgacgc gcgcacgcgg atcgatatca
    15541 atatccagtt catcaaaaat cgctttggtt tcgcggtaca ttttgtcctg atcgacaaac
    15601 atgcctttgg tgggatatcg ccccagccac atgttgtcca tcaccgaacg ttgtaatacc
    15661 aggtttaact cctggtgtac catcgaaata ccattttcca gcgcttcttt ggcagaatgg
    15721 aaatcgatct ctttaccctg gaataaaatg gtgccggagt ctttttgata aataccaaac
    15781 aggcatttta ataatgttga ttttcctgcg ccgttttccc ccattaatgc atggatagaa
    15841 tgtggccgga cttttaaatt aacgttatca agtgccttaa cacctggaaa agacttgttg
    15901 ataccgctca tttccaacaa gtattccccg gaagacggag tcgttgagct gaccatataa
    15961 ttttaccttg ttggccatac aataagggcg cagtaaacga ctgcgcccaa tcagtcttat
    16021 ttcttgctga attcagccag gttgtcttta tctacgccaa cataaggtac gcggaccact
    16081 ttgttgtcga ttttccagtt ggtgccatca gccgcacctt tgccatcggc caggtttttc
    16141 gccagatcaa aggtcgcttt cgcctggttg ttagcatcgt tcagtacggt gcccgccagt
    16201 gcaccggatt tcaccagcgc cagcgcttct ggcagcgcat cgacgccaaa caccggaatg
    16261 ctggacttgt tgtgtgcttt cagtgcttct accgcgccca ttgccatcgc atcgttgttg
    16321 gcgataacca cttcgatttt gttggcgttc gggccagaca gccaggcgtc catcttatct
    16381 ttcgcctgag cggtatccca catagcggta tctaactgta actgttcagt tttgatgcct
    16441 ttgtcgttca attctttaat cacgtaagtg gtacgtgctt ctgcatccgg atggcccggc
    16501 tcacctttca gcagtacgaa ctgaatctga ccgtctttgt tcagatccca accctgattc
    16561 gccgcccagt gtttagcaat caaatcgccc tgaataatgc cggactcttt ggagtcagtg
    16621 ccaacgtagt aggctttgtc gtagctatcc agcgccttac gagacggttc tttgttgaag
    16681 aaaaccaccg gcacgttttg cccacgcgct ttctcaatca ccgtacccgc agctgccggg
    16741 tcaaccaggt tgattgccag tgccttcacc cctttcgcca gcaatacgtc gatctgatcg
    16801 ttctgcttgg actggtcatt ctgagaatca ttcatcagca gctgaacatc tggcgcggct
    16861 ttcgcatctt gctcaatagc cttgcgcacc acagacataa agttatcgtc gtacttatag
    16921 attgttacac caatgcgagt atcagcagcg tgtgcagcgg caccgaataa catgctggcc
    16981 atcacagcaa acagggttaa caccttctta ttcatggtat ctccggtttt tcttatgcag
    17041 ggtagtgctt gagataaatg ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc
    17101 cgaagctcac tttttaaaat tcgttcttcc atgcccggta acgctccaga aaacggcttt
    17161 aattgttgtt atgacgctat tacctcggtg aaagtgatta ttcactgtta catacgggtt
    17221 acaacgttaa aacggtgcaa tcatagctat cacattgtta agatactgtg aaatcactca
    17281 cagattgaaa gcggttacat cgccagattt gttgagttag tgatcgccgc cgcattctgg
    17341 cgcgttgcga cagaatggcg acgcactaac gtcggcataa aacagtggct ggcacgcgga
    17401 tcaatattgc ctgctgcccc ctgcaaggcc agttcggtgg ctaatttagc cattgaagca
    17461 atgggataac gcacagtcgt taattgcggg tcggtgtaac gggcgatggg aatatcatcg
    17521 aaaccgatga tggagagatg taacggaatc gcaatgccat tatcttttaa tgctgtcagt
    17581 gcgccagcgg ccatattgtc gttataagca aatacagcgg taagttgtag attgcgcccc
    17641 agcagttcaa ccatcgccgc ctcaccgccc ggcatgtccg gcgtacctgt gccaatccag
    17701 ctttccggcg gaataatatc ctgctctttc aacgcactca tccagcctgc tttacgcatg
    17761 gcgtcatctt caatgccgtg actggaagaa agataaccaa tacgttgatg accgttattc
    17821 agcaacatgc gcgtcgccat tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg
    17881 gcgtaccccg gcacaacgcg gttgattaac accataccag gaatgttatc cataaattgt
    17941 gccagttcat catcactcaa tgcttttgag tgaacaatca acgcattaca ccgctggcga
    18001 attaacacct caatggcgtg acgctctttt tccgcttcat gatagctatt gcctattagc
    18061 acgtatttct gatgctgctg ggcgaccaaa tccaccgctt ttaccagcgc accaaaaaac
    18121 gcatcagaaa cgtccatcac caccacgcca atggtatcgc taacctgagt tgccagcgcc
    18181 tgggcattgg cgtttggccg ataatccagc tcactcacgg ctttcattac tgcttcacgc
    18241 gtgtcggcac tgaccagcgt gctgttattg agcacccggg aaaccgttgc cacagagacg
    18301 ccagcctgac gcgctacatc acgaatggtg atcatattca ctacctgtta tgagattgca
    18361 gtaaatgact gcttgctggc ggctattttg tcagcactga atacaggact tcgtgaatcg
    18421 agtcacagca atggaaacgg ttacagccgt tttgttaacg aatgtgatcc agatcgttat
    18481 cttgatgttt tagatcttgc cggcccagct gcacgcaaag ttaactgacg ccagagccat
    18541 tccaccggcc cctggcggaa gtaacgcagc cagataacag agaagaggat attcgccagc
    18601 cataccggaa taacaaacgc cagcagctcc aggcggtcaa aatgcataaa caaaccgagg
    18661 tggtaaaaaa gcgtggtaca aatcagcgtt tgcaatagat agttggtcag cgccatccgt
    18721 ccgacgcagg cgatcgcaag caccagctta aagcggctca attgcggcca gaagccataa
    18781 aacagcgacg catagccaat cgcctgaaac ggcgcactca gttcccgcgg catctgaagt
    18841 aaaaaggcac accagcgata cgcccagtcc agctgccatt gcagggcgat ggcaggaagg
    18901 ttaatggtca ccccaatcgc caccagcaca aaaccagtac gacgatagtg acgtaagctg
    18961 aactgccctt tcagccagcc gctgcgcatc agcgccgcac caatgagcat catccctgcc
    19021 agttgccagc catactgtgc gcccagtgcc agtaaactgt tgccaacacc atcggcacga
    19081 ttactgatcg cttcaacacc accgtgaagt ttccagtact tttcatacaa aatagccgat
    19141 gcatcaggtg tccaggcgcg gctggtctgg ctatcggaaa tcaaccccaa taacagcaaa
    19201 acgccaagcc ccaccagata aagcatgacg ccggtattaa acaggctttt taccgatggc
    19261 gcatcgcgca ccagccgcca gcagattaag cccaccagcc cgtaagccag cagaatatcg
    19321 ccgtcccaga acaataaacc gtgaataaag cccagcaaga ctaacagcgt taaccgcgac
    19381 tggatccagc gtctgccacg gggcagcaac atttgcaggc cagcaccaaa cagcagcgca
    19441 aaaagcgtga ggaatttcac ctggccgatg agatcgagaa atgcccaggt ccatgcatcc
    19501 tgcggcgtaa tagcgccgta ccaggcggga ttaagataag ccgcctttgg tagcccaaag
    19561 gcgctgatgt ttaatagcag gatccccaga atggcgacgc cgcgaacaaa atcgagcgtg
    19621 acgttgcgcc ccatggttcc tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa
    19681 ttcgtggcgc gtattctgac tggatttgaa caatccacca agagaggtcg ttgttgtggc
    19741 actggttgcg tcgcggatgc cacgcgcttt cacgcagtaa tgcaccgcgt cgatagagac
    19801 agccacgtta ttggtgccca gcagcgtttg tagcgcaatg agaatttgct gggtcagacg
    19861 ttcctgtacc tgtgggcgct gggcaaagaa ctgcacgatg cggttaattt ttgacagacc
    19921 gatcaccgaa tctttcggga tataagccac cgtcgctttg ccatcgatgg taacaaaatg
    19981 gtgttcacag gtgctggtca gggtgatatc gcgcacggtg accatttcat cgaccttcat
    20041 tttgttttca atgagggtga ttttcgggaa attggcgtaa tccagaccgg agaaaatttc
    20101 atcgacatac attttagcga tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc
    20161 gagattcagc agctgcatga tttcggtcat atgaccagca ataaggcttt tgcgcgtttc
    20221 gttatccatt tcatgcacgg gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc
    20281 ttcatgaacc agggccgctt ctttactgag tgatggcatt tatgatttct cctgcaggtg
    20341 tgacgcctcc gccctgcgtg ggggcaaagt tattaagctg atttacagcc tgattattgt
    20401 gcgtgaggcg gcgcacataa tccagtattc acagcgataa ttattgtaat tgccgctgcc
    20461 tttcatcagc agatgttaaa acatcgttat gcaaatacgg aagtgaaagt tactcacagc
    20521 acattgaata aacggtatga tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg
    20581 aaatgctcga agagcaccgc tgttttgaag gctggcagca acgctggcga cacgactcca
    20641 gtaccttaaa ctgtccgatg acgttcagta tctttctccc accacctcgt gatcacaccc
    20701 cgccaccagt gctgtactgg ctttccggat taacctgcaa tgacgagaac ttcaccacca
    20761 aggcgggtgc ccagcgcgtg gcggcagaac tggggattgt actggtgatg ccagacacca
    20821 gcccgcgcgg cgaacaggtt gccaacgacg atggctacga tttaggccag ggcgcaggct
    20881 tttatcttaa tgccacgcaa ccgccgtggg cgacgcatta ccggatgtat gattatctgc
    20941 gcgatgaatt accggcgctg gttcagtcgc aatttaatgt cagcgaccgc tgcgctatta
    21001 gcggtcattc aatgggtggt cacggtgcgc tgattatggc gctgaaaaac ccgggcaaat
    21061 acaccagcgt ttccgccttt gcgccgattg tgaatccatg cagcgtaccc tggggcatta
    21121 aagcgtttag cagctattta ggtgaggaca aaaatgcatg gctggaatgg gacagttgtg
    21181 cgctgatgta tgccagtaac gcgcaggatg cgatcccgac gcttatcgat cagggtgata
    21241 atgatcagtt tcttgccgac cagctgcaac ccgcagtgct ggcagaagcc gcccgccaga
    21301 aagcgtggcc gatgacgctg cgtattcagc cgggatatga tcacagttac tacttcatcg
    21361 cctcttttat agaggatcac ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg
    21421 cccggttcgc cgggcatctt ctcatcagaa gcgataatcc actgccataa agtaacgacg
    21481 tccgtcttcg ttatagctgt agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac
    21541 gcctgcgcgc agtttgacgt ctttagtcac ctgccaggcc gcaccggtat tccagatggt
    21601 ataaccgccc ggtgttttag ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgtcc
    21661 ggaaacatag aatgaccagt cttccagcgc cagcggtttc cagtccagcg taccgttagc
    21721 ggtatggaac ggcagatcgg ataaaggttt attttcgccg ttgctgacat cacgaccatc
    21781 gttgtaggtg tagttgagtg acagtttcca ttcatcgttg aacggaattt tcagctcggt
    21841 ttccacgccc tgaatacgtg ctttgttaac gttgtagtag ctaaataccg gcacaccgtt
    21901 cgctgtaaaa ccaacaaagt tttggtagcc tggtgcagca ttgacgtcag acgtacggct
    21961 gatgctgata cgatctttca catcgttacg gaaaacggta acgctggatt caaccccttc
    22021 cagccagcct tcttcgccca tgtagtaaag ccccagctcc caactttcgc tggtttctgg
    22081 tttcagatcc gggctaccca caatcttaca tgcgccacgg caggaattgc tcgtccagtc
    22141 agggctaagt tgcaacagag aaggtgcttt aaatgccgtc gcccagcccc ctttcaccgt
    22201 tacggtgtcg gtggcgttat aaaccaggta ggcacgcgga ctccagtgtt caccgtaggt
    22261 ttcatgatcg tccatacgta cgccggtcgt cagcgccagc ggctcgaaga tccgccattc
    22321 atcttcaaca aatagcgcgt actggctggc agatgttttg gagctggttc ccccagtcag
    22381 gttcaccgca tcgctaagtt tgtcgtgacg ccattcaccg ccaaccgtga gaaactgatt
    22441 aatcgccgtc agcggcaacg tgtatttgcc gtcgaccgta ttgctttcgg aagttatcgg
    22501 gctgctgttg ccagggtttt tgttctcgac tttctcaccg tagtatttca gttcgctggt
    22561 gccgtaatcc caacgcccat tatggctgac ggagtagttc tggcgttcca ggcggttttt
    22621 gtccagcgag tcggaatcac gatcctgacg gtcgaaaccg tatccggcag taaaatcgtg
    22681 attttgattc ggtgtccagg caaattcgac attgccgtcg cggctggaga atccttcaat
    22741 acgcggcgtt tctccggtat cggtggtcgt tgagttttgc gggtcatcct tttcacgttt
    22801 tgccaggctg ccgtaagctt tcattcccag cacgccgtca attaatggtc cgctggtaaa
    22861 gaattgaccg ttataggtat caccgcgatc gcgatgttcc tgaacggtgg tatcgacggt
    22921 aacggtgccc gaccatttct gaccgatttt tttggtgatg atattcacta caccgccgag
    22981 cgcatcggaa ccgtacagcg acgacatcgg gccacggacc acttcaatac gttcgatgga
    23041 atcgaccggg atccagttca gatcgaaatc attgtggcgg aagacggcat tgcgggagtt
    23101 aacgcgttta ccgtcgacga gaatcagggt gtagctgctg tccagaccac gaatactaac
    23161 gcccttacgg ttatcccctt cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc
    23221 cttcagattc tgtaccggtt ttcgctgcag gtcttcctag gtaatgacgc tgatactggc
    23281 aggtgcatct ttaaggtttt gctcaacgga agatgcagtg acaaccatcg tttcgccatc
    23341 atcatcgacc gctaacacag gccatgcaca agaaatagcg gacaaacaca gcccgacccg
    23401 tacgaaaggg ttcaacctaa acattccata tctccatgag gtaactacga aaataaaatg
    23461 ggttatcgct cacatcttct tcacgtcccc ttgcgtacgg cagcatcgcg gtggacttat
    23521 ttgtatgcag gtgatcatcc cagaaagcca gaccagcctc tgatttgtca gccttttttg
    23581 attgcggcgt aacgataatg caaacgataa caattatcaa tccaaatgtt aaattttata
    23641 tccgcatgat ttgtcaggga aatggaattg aaaaagccct ctcggttgag agggcttagc
    23701 aaggaaggga gaaaacttat ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta
    23761 caaagtgagt tcctttaatc agcttgtagc cgaaccaaat aatcaggaac agcgggatac
    23821 caatatacgt tgccgctacg ccgccccagt caatggtatc tttcaggaac gcttcatagt
    23881 tctggcccaa agtgataatc agacacagaa tgaatgcgaa gatcggcccc agtgggaaga
    23941 aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa ccgcgacgga
    24001 agcgatagtg gctaacggca atccccagcc aggcgataaa accggtcatc ccggaggtgt
    24061 tcagcagcca caggtatacc gtctggttgc caaacatgga ggtcaggaag cacagaccgg
    24121 caatcaccgt cgtcgcatac agcgcattac gcggcacgcc accacgcgac agtttagcga
    24181 aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag catacgagta gacgcataca
    24241 taccggagtt acccgctgac agcaccgccg tcagaataac tgcgttcatc accgctgcag
    24301 cagagagcag acccgcgtgc tggaacacca gggtgaacgg actgacgctg atgtctttaa
    24361 catcgttacg cagcaggctc ggatcggtgt acggaataat caggctgata atcaggatcg
    24421 cgaacacata gaacaacagg attcgccaga acacctgacg taccgcgcgt ggaatgtttt
    24481 tcgccggatc ttcggactcg cctgcagcaa taccgatcag ctcggttccc tggaaagaga
    24541 agccgacaat catagctacg ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc
    24601 cgattgtcca gttgctccag cccgcaggct gcgcgccttt gaagataccg ataatcatca
    24661 gcacgccaac gatgataaag acaataactg tcgtgacttt gatcagtgag aaccagtatt
    24721 ccgcttcacc aaagccacga actgagatgt agttcagcag gaagataacg ccgaggaaca
    24781 gcgcactcca gatccagccc ggtgtatccg ggaaccacca gctcatgacc agctgagctg
    24841 caaccaggtc aacggcgata gtcaccgccc agttgtacca gtagttccag cccagcgcga
    24901 agccaaagcc ttcttcaaca tagttctgac cgtaagtggc aaacgaaccg gaaaccggca
    24961 tatatgcagc cagttcaccg agactggtca tcaggaagta aaccatcagg ccaatcaaca
    25021 tatacgagag caatgccccg cccggacctg cctgagaaat cgttgcgcca gaggcaacaa
    25081 aaagacctgt accgatggaa ccgccaatgg caatcatcgt caggtgacgc gcctttaatt
    25141 cacggcgtaa gcccggcgct tctgtggttt tagtttcgga aaccatacaa aaatgctatc
    25201 catcttaaaa atgaggcgcg attgtagcag acgattggca atccttccgg cagaaataca
    25261 cggttataag acaccttcat gatcgcccag ggattataag taaagcagcc aatctctttt
    25321 ctggcgaatg catgctgagg gcgctgcgtc acgaaatcga cacagcacca gcatgttctt
    25381 gtacagcaac ttaacgcggt acattcgcgg gatcgcaata gtccagaaag cgccgtagcg
    25441 cgttggaaag gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc aggcgcggca
    25501 gagggaccgc aacttcactt aatgtgcctg cctgcaattg atcttcaatc acccgtcgcg
    25561 acaggcaact aattcccaac ccatgacgca ccgcatgttt gattgcctcg gagttaccta
    25621 attccatcgc catctcaaac ttcggtaaat gtgacagcaa cagataatcg acaatctccc
    25681 gcgtgccgga gccgcgttca cgcaggatcc acggcgcagc ggccagctgt tctaaggtga
    25741 ccggaccacg agccaatggc gaagtcggcg cggcgaaaac caccagctcg tcttccagcc
    25801 acggttcaga aatgatttca gtgctgtggc acggtccttc aataaagcca atatcaacgc
    25861 ggaaatccag caccgcttgc atcacgtcct ggctgttccc aacgctaagt tcaatcggca
    25921 actgcggata atcatggcga taacgggcaa tgactgcagg cagaatgtag ttaccgatag
    25981 tactactggc atagatacga atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa
    26041 ccgcctgttc aagcagtgcc aacgcgcgcg gatagagcag ccgcccgtgt tcattaacaa
    26101 ccagtctttt ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc agatcggtca
    26161 aggctgcgct cactgctgat tgcgacaacg ccagcatcac cgacgcctgg gtggttgatc
    26221 cacttttcaa tacttctgca aaaacttcca actgccggag ggtgatgtgc atagtcgctt
    26281 accacttata aagattaatt ataaatatat aatcaatttt atttttaaac cagttagtcg
    26341 taaccttatg cccggttaaa ggagagggtt atgacgaata tcaccttaca gaaacaacat
    26401 cgtacactgt ggcattttat tccggggtta gccctgagtg cagttatcac cggggtcgcc
    26461 ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt ttagtgccct caccctcgca
    26521 atcttgttgg ggatggtttt aggcaacacc atctatccgc acatctggaa aagctgtgac
    26581 ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg gtatcattct ttatggcttc
    26641 cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg ggatcatcat tgacgtgctg
    26701 acgctgtcca gtaccttcct gctggcttgc ttcctggggc agaaagtgtt tggtctggat
    26761 aagcacacca gctggttgat cggtgccggt agcagtatct gtggtgctgc cgcggtactg
    26821 gcgactgagc cggtagtgaa agcggaagcc agtaaagtaa ccgtggctgt tgcaaccgtt
    26881 gttatcttcg ggaccgtcgc gattttcctc tacccggcga tatatccgct gatgtctcaa
    26941 tggtttagtc cggaaacttt cggtatctac attggttcta ctgtgcacga agtggcgcag
    27001 gtggtggcgg caggtcatgc catcagcccg gatgcagaaa acgcagcagt tatttccaaa
    27061 atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc tggcggcgcg tgttaaacag
    27121 ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta ttccgtggtt tgccatcttg
    27181 ttcatcgtag ttgccatctt taactcgttc cacctgttac cgcagagcgt ggtgaacatg
    27241 ctggtaacgc tggatacctt cctgctggca atggcgatgg cggcgctggg tctgaccact
    27301 cacgtcagcg cgctgaaaaa agccggggcg aaacctctgc tgatggcact ggtactgttt
    27361 gcctggctga ttgttggtgg tggtgcgatt aactatgtga ttcaaagcgt catcgcataa
    27421 accactacat cttgctcctg ttaacccgct atcattaccg ttttcctcca gcgggtttaa
    27481 caggagtcct cgcatgaaat acattggagc gcacgttagt gctgctggcg gtctggcaaa
    27541 tgccgcaatt cgcgccgccg aaatcgacgc aaccgcgttt gccttgttca ccaaaaacca
    27601 acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc gatgaattca aagccgcctg
    27661 tgaaaaatat cactacacat cggcgcaaat tcttccccac gacagttatc tgattaacct
    27721 cggacatccg gtcgctgaag ctctggaaaa atcgcgcgat gcgtttatag atgaaatgca
    27781 gcgttgcgaa cagctggggc tttctttgct caacttccac cctggcagcc atctgatgca
    27841 gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc atcaacattg cgctggataa
    27901 aactcaaggt gtgacagcgg tgattgaaaa caccgccggt cagggcagta acttagggtt
    27961 taaattcgaa catctcgcgg cgattatcga cggcgtggaa gataaatccc gcgtcggcgt
    28021 ctgcattgat acctgccatg cttttgctgc cgggtatgat ttgcgtactt ctgccgaatg
    28081 cgagaaaacg ttcgcggatt ttgcccgtat tgttggcttt aagtatctgc gcgggatgca
    28141 ccttaacgat gcgaaaagca cctttggcag ccgcgttgac cgccatcata gcctcggtga
    28201 aggcaatatt ggtcatgatg cgttccgctg gatcatgcag gacgaccgtt tcgacggcat
    28261 tccgctgatc cttgaaacca tcaacccgga tatctgggca gaagagatcg cctggctgaa
    28321 agcgcaacaa actgaaaaag cggtagcctg aagatgaaca accgggagaa ggagatcctt
    28381 gcaattttac ggcgtaaccc gctgattcag cagaacgaaa ttgcggatat gctgcaaatc
    28441 agccgttcgc gcgttgcggc gcatattatg gatttaatgc gcaaaggccg gattaaaggc
    28501 aaaggttaca ttctcaccga gcaggaatac tgcgtagtgg tggggacaat caatatggat
    28561 attcgcggga tggcggatat ccgttacccg caagcggctt ctcatcccgg taccattcat
    28621 tgctcggcag gcggcgttgg acgcaatatc gcccacaacc tggcgctgtt aggccgcgac
    28681 gtccatttgc tttcagtgat tggtgatgac ttttatggcg aaatgctcct ggaagaaacg
    28741 cgccgcgccg gcgtgaatgt ctccggctgc gttcgtttac atggccaaag cacatcgacg
    28801 tatctggcaa ttgccaatcg agacgatcaa accgtgctgg cgattaacga tacccatctg
    28861 ctggatcagt tgacaccgca actactgaac gggtcgcgcg atttacttcg tcatgcgggc
    28921 gtggtacttg ctgattgtaa cctgacagcc gaggcgctgg aatgggtctt taccctcgct
    28981 gatgaaatcc cggtgtttgt cgataccgtt tcagaattca aagcgggcaa aatcaaacac
    29041 tggctggcgc atattcacac cctgaaaccc actttaccgg agctggaaat tttatgggga
    29101 caggcgatca ccagcgatgc tgaccgtaat gccgcagtga atgcgttgca tcagcaaggc
    29161 gttcagcaac tgtttgttta tttgcccgat gagtcagttt attgcagcga aaaggatgga
    29221 gagcaatttt tgctgacagc gccagcgcat acgacagtag acagttttgg tgctgacgat
    29281 ggttttatgg cgggcctggt atatagcttt ctggagggaa acaatttccg tgacagcgcc
    29341 cgttttgcga tggcctgcgc ggcaatttca cgcgccagcg gcagcttaaa caaccctacc
    29401 ctgtctgccg ataacgcgct ttcattagtg ccgatggtgt aacaatgttg ccggattcga
    29461 cgcttgacta catcttatcc ggctcataac tcgcgcacta cgctaagcca ataaagaatc
    29521 ctgcaatggt tgcactcatc aggttagaaa gtgtcgccgc cgccagcgcg cgtaaaccaa
    29581 gctgggcgat ttccggtgca cggtgtggcg caaccgcaga aaacgccccc accaccaccc
    29641 cgatagaacc aaagttagcg aaaccgcaca acgcgaagga aataatcgcc acggttttag
    29701 catccagagt gccagctgtt tgcagataag gtgagaaatt gaggtaagcg acaaattcat
    29761 ttattgccag tttctgtcca atcaaactcc cggcaagatt cgcatcactc cagtcaacac
    29821 ccattaccca cgccagtggg gccagcaggt aacctaaaat ggactccagc gaggcatgtt
    29881 caaagccaaa ccagccgcca acgccgccga taataccgtt aattaacgca atgatggcga
    29941 caaatgccat cactactgtc gccacacctg cggcgatttt cagcccggtc attgcccctg
    30001 tcgcagcggc ttcaataatg ctttttggcg gtgtttcggt gaaagagagg ttattaaagg
    30061 aaacctgcga agattctgtt gccgggctta acaggcgggc aaacaagatc ccgccgggga
    30121 tcgccattaa cgatgccgcc agcagatatt ccacaggcac gcccagtgcg gcgtaaccaa
    30181 tcattgtcga accagcaatc gaggccatcc cgctacaaat cgctgtaaat aattcattgc
    30241 gattcagacg attgataaag gatttgacga ttgccggaat ttcgttttgc ccgaggaaaa
    30301 tggtagtgac cgcgacgaat gactcgatct tgctgatatt caatgctttc tggaagatac
    30361 cgccgagaat gcgaattaag atccccatca caccgatgta gtagagaata ctcaccagtg
    30421 ctgtaacgaa gataattgcc ggtaataccc tgaaaccaaa gataaatcct gcgccatcaa
    30481 ataaggtatc catttttggt ccgaccagag aaccaaagat aaatgcgcta cctgcatcgc
    30541 tgtacgccat cactttatgc acgccaaaag cgactttttc tgcgacccaa cgccctggtg
    30601 gtaaccaaag cataatgccg ccaatcacga cctgtaacac taacgccgcg ccaacggtac
    30661 gcaggctgat cttcttcttg tttactgaca gcaaaaacgc aattgccagc aataccacca
    30721 ttcccagaac acttctcatg acatccatag ttattatctc ttcatgtcat tttgggcgac
    30781 ggctttctgc ccatcagaaa tagccgggcc tgcatcttgc gatactgtcc cggcttgtta
    30841 tcaacactta atgggttttg atgtagccgc gcacgcactc ttcgactaat ccccagaacc
    30901 agtcagtatc aatagtgatg ccgactttgg tattggcggg cttgccaaga acgcccagct
    30961 cgtcgcagac cgtacgcccg tagcaagggc cgctgttaac gtccacttcg acgtacatct
    31021 cctgggtttt aatgccatca gggttaatca gataaccgat gcaggtggcg tcatgcaccg
    31081 ggccgccagc caggccgtag ttttcgaact gggttttgag agtgaagttc atgatgtcgc
    31141 tgaacagctc tccggcaggg ccgcctgccc tttccatccg agcaatcacg tccggggtgc
    31201 aaacggtctg gttggtgaga tcgaggccca tcatcactaa tggaacgccg gaggtgaaca
    31261 ccacgcgtgc ggcttccggg tcagcaaaga tgttgaactc ggcagatggg gtgaagttgc
    31321 cagtaccgta agcgccgccc atcagcacaa tttcacggat tttcggcagg atcgcgggtt
    31381 gcatacgcat tgccaccgcg atatttgaaa gtgggccaac cggcaccaaa gtgatatcgc
    31441 catcgcttgc catcagggta tcaatgatat atttcaccgc atgagtgctt tctgcctggc
    31501 gggtcagcgg ctcgaatacc gggccatcca gtccggtttc accgtgaata ttatcggcaa
    31561 cgatttgttg acgcataatg ggctgtggca tccccgcata aaccggaaca ttaatctcca
    31621 gtttctggca aacattcagg ccattaatta atgttttatc aagcgtctga ttacccgcca
    31681 caatggtgat acctaataaa tcaatcgccg gatgtttcgc tgccatcatt atggcaatag
    31741 catcatcatg acccggatca caatccagaa taatttttct cttttccatt gtttatttcc
    31801 tctgtttcca gttgcgttat tttttctaca gcaaagaaaa ttcgaccggg ccgatgattg
    31861 aatcttaaca acagcgtaag tatgctaaat atgagaaatc tcatagcgga taaacatcgt
    31921 gaaagaaatc cacaataatg accttaagca gcaattgatg agtgaatctg cgtttaagga
    31981 ttgcttttca acggatgttt cagccgatac gcggctgttt cattttttag cgcgtgacta
    32041 cattgtgcag gaagggcaac agccgtcatg gctgttttat ctgacgcgag gccgtgccag
    32101 gctttacgcc acgcttgcta atggtcgcgt gtcgctgatc gatttctttg ccgctccctg
    32161 ttttattggc gagattgagt taatcgacaa agaccatgaa ccgcgtgcgg tgcaggctat
    32221 tgaagagtgt tggtgccttg cgctccctat gaaacattac cgcccgctgt tattaaacga
    32281 cacgctattt ttacgaaaac tctgcgtcac cttaagtcat aaaaattatc gtaatattgt
    32341 ttctttaact cagaatcaat catttccgtt agtgaatcgc ctggcagctt ttatattact
    32401 ctcgcaggaa ggtgatcttt atcacgaaaa gcatacgcaa gcggcagagt atttaggtgt
    32461 ttcttatcga catcttttat atgttctcgc gcaattcatt cacgacggtt tattaattaa
    32521 aagcaagaaa gggtatctca ttaaaaacag aaagcagttg tcaggactgg cactggagat
    32581 ggacccggaa aataaattct cagggatgat gcagtaaaaa ttatttgcaa tagagcgatt
    32641 gccggatgtg acgcttaata agcgcgtaca tccggcaatg acactcaagc taaaccaata
    32701 aagaacccgg caatggtcgc gctcatcagg ttggaaagcg ttgctgctgc cagcgcccgt
    32761 aaaccaagct gggcgatttc cggtgcgcgt tttggcgaaa tagccgaaaa tgccccaacg
    32821 acaacaccga tagaaccaaa gttagcaaaa ccacaaagcg caaaggagat aatcgcaatg
    32881 gttttcactt ccagcgtgcc gcccgtttgc aggtatgggg agaaactcag gtaagcgacg
    32941 aattcgttaa tcgccagttt ctgcccaatc aggctacccg caagattggc atcactccag
    33001 tccacaccca tgatccacgc cagcggtgcc agcacatagc caaaaatact ttccagagag
    33061 gcattggcga aaccaaacca gccgccaatt ccgccgataa tgccgttgat cagcgcaata
    33121 attgcgacaa acgccattac aaccgtcgcc acaccagcgg cgattttcag cccggtcatc
    33181 gcaccgctcg ccgccgcttc gataaagctt tttggcggcg tttcgctgaa cgacagattt
    33241 tcaaatgtca cctgcgaagg ctcggtggct gggctaagaa tacgtgcaaa caaaatacca
    33301 ccagggatcg ccatcagcga tgccgctaac aggtagtcaa ttggtacgcc cattccggca
    33361 taaccaatca tcatcgaacc agcaatggac gccatcccgc tacaaattgc ggtaaacaac
    33421 tcgttgcgat tcatgcgatc gataaacggt ttaacgatcg ccgggatctc attttgcccg
    33481 aggaaaatag tggtaaccgc aacaaaagat tcgattttgc tgatgttgag ggctttctgg
    33541 aaaatgctgc caaggatgcg aatcagcagc cccatcacgc caatgtagta cagcagactg
    33601 atgagcgcag tgacgaagat aatcgccgga agtacgcgaa aggcgaagat aaaacccgca
    33661 ccgtcaaaga ggacatccat tttcggcccg accagcgaac caaaaatgaa ggcgctaccg
    33721 gcatcactgt aagacatcac tttatgaacg cctaatgccg cctgttctac tgcccatttt
    33781 cccggtggga agtagagcat gatgccacca atagcgattt gcagcagcag tgcggctcca
    33841 accgtgcgca aactgatgct ctttttattc actgacaaca gaaatgctat tgccagtaac
    33901 accaccatcc ccacaacact tctcattata tccataatga ttttcccttc atgccagtaa
    33961 acccggcgtc agcgccaggt tttggtatgc ttgatgagta cgggcgacgg ctttctgccc
    34021 gtcagaaatt aacccgcgag acgctggtat tctttggcta tttcactcgc caggatggcg
    34081 ttgttgaaca ccagctggat gttggatttc aggctgtcac cgccggtcaa ctcagcaacg
    34141 cgcgccagca ggaatggcgt actttcttta ccaataacac cctgcgcttc agcttcagct
    34201 accgcctgat cgatcgccgc attgatagtg tgttccggca tagcaaactg ttccgggatc
    34261 gggttcgcta ccacgaggcc accgttcagc ccgctttgcc atttcaccgc cattgcacgg
    34321 gcaatttcgc tggcgctgtc gagacgaatg ctcacgtcaa acgggctggt gcggcagaaa
    34381 aacgcaggca gcgctttggt ctgatagcca attagcggta caccgaaagt ttctaaatat
    34441 tcggtagtta agcctaaatc gagaatagat ttcgccccgg cacaaacgac ggtgacattg
    34501 gtgtgcgcca gttcttgcaa atcggcagaa atatcgaagg tatgttccgc cccgcgatgc
    34561 acaccaccaa ttcccccggt ggcaaatact ttaattccgg caagcgccgc aataatcatc
    34621 gttgaagcaa cggtggttgc accatttttt ccggcggcaa caacaaaagg taaatcgcga
    34681 cgactaactt tggtcacgtt atgcccttca cgacccagca attcaatttc ttctttgctt
    34741 aaacccactt tcatcacgcc gccaataatg gcgatagtgg caggtacagc gccctgttta
    34801 cgaatagttt cttcaacttc aattgcggtc tgggcatttt gtgggaacgg catcccgtga
    34861 gaaataatgg tcgattccag cgccacaacc ggttttttgt tttttaaagc gtcctgcact
    34921 tccggggaaa tttgtaataa ttcaggggaa atttttaatt cagacattct gcgttctcca
    34981 ctaacgatat aacgttggca atcgataaat cggggttatt ggtgtattca caggagagcg
    35041 ccattgacga acatccctgt gcgaaacgaa cagattcggc aaacggcatt ccgtctaccc
    35101 aacacgaagc aagtcccgcc atcatggcat cgcccgctcc ggtaacatta atgacattgg
    35161 ttttgatcgg cgcagaccaa ccactttcac cgctgatatc actgtaataa acgccgtcgc
    35221 cgcccatgct caataccagt cggttcaggc catgttgatg gaaccaggca gcaacttttg
    35281 ccacatcttc acgccctgac aaagcaatcc cactcagggt ttccgcttca aggcggtttg
    35341 gcttgagagt gtggatttga tttagacggt cgcgcacttt gacacatttc cacgcggaaa
    35401 caggatcgac aaatacgggt acgttggcgg cattatccag aatccatgcc agtgcctctt
    35461 cactgatatt acagtccgcg acaatgacct ttgccctctg aataaattca ccgtgctgtg
    35521 cgagatattc agctgtaata gcgttgctaa tattcatgtc atttatagca accagcattt
    35581 caccggtatt atcgagtaat gataaatagc tcgacgtatt ttctcccggc acaatcaggc
    35641 atttatcgac ataaacacca gattgattgg tttgcgttag cagcgattga ccataaaaat
    35701 cactgcctac ggcgctcagt agccaggctt tattccccag caacgccagg ttttgtgcaa
    35761 tattgcgccc tactccacca ggcgtaaact ttattttacc tggatttgaa tccgcataat
    35821 ttaatgattc atgtgaatat ccggcgacat caatattcgc cgaacctata attacgacat
    35881 aatccttttc gcgcatagca cgtcctctgg taaaaaatta ccaatcctgt caatccacaa
    35941 gaatatagat actgcaaaga cggtttaaac atgtgttcat atttaaacat gtgctcatag
    36001 tatgcatgtt ttacgtaaag taaattgccg ccagagaggc aaaatgggca gatttgagca
    36061 ggctcacaaa atgcgattaa atgcctgaat tacaaaagtg aggaatttgt gacgggatgc
    36121 acagaacagg aacactaaaa aagggcagaa aataatctgc ccttgaggaa ataaccttag
    36181 tgtttgatga acatttcagg tttctactga aagataaaac catttaaatt cataaaatta
    36241 cattcacaaa ctggtcattc cctttgcgct catcaaatgt tacagggcag gaaatttctg
    36301 ccctgtaaca caccttttat tacgctgctt tcgctactgc gtccacttcc ggacgtttca
    36361 ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc aggtaaccca
    36421 gtaccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgc ggtgccataa
    36481 gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccaccaacg atacaacacg
    36541 gcagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa ataaagcaca
    36601 gaccaagaac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat ttgcgacgcg
    36661 ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc ataatcgccg
    36721 ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac gctgctttat
    36781 ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc agcagaaccg
    36841 cattcgcagt ccccatggtc tgcagccagt gagtcaaccc ttcgagaatg ccagcaaccg
    36901 gtttaccgat caggtagatc atcgccagac cgaccaccag actggaaatt agcggaatga
    36961 tcaggatcgg tttcagcgcc tccatactct gtggcagttt cagttgcgta ctgattaact
    37021 tcgcaatgta accagccagg aagcccgcaa taataccccc aatgaagcca gaaccggtgc
    37081 tgaccgccag cataccccca atcagacccg gcgtgagacc cggacgatcg gcaatggaga
    37141 aggaaatgta acctgccagt accggcacca tcagcgcaaa ggctgaacca ccaccaatct
    37201 gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca aaagcaaaag
    37261 aaagcgcgat acacagacca cctgcaacga ccatcggcag catgtaagag acgcccgtca
    37321 gcaagtgacg gtaagcgcct gcaccctctt tcttaccttc agtggtcgct gtttgagctt
    37381 tgcccgccgg ttcatacggc gttgcttcag caaccgcttt atccagttcc tgcgcggttt
    37441 tcttcagcgc cagaccggta gaggtgcgat acatcggttt accagcaaat ttcgccagat
    37501 ccacttcgat atctgccgcc acaatcacca gatccgctgc ggctacttct tcaggggtga
    37561 tcgcattacc cgcgccaaca gaaccacggg tttcaacttt cacccaccag ccacgttttt
    37621 tcgcttcggt ttcaatggct tcagccgcca taaatgtgtg tgctacgcca gtcgggcaag
    37681 ctgtcaccgc aactacgcgt ttcggaccgc tggtggcaac tggtgctgtc gcagcgaccg
    37741 gtgcagtgta aggtttcgca tggcctttgg cttcactcag gaacagctca gggtgcgcaa
    37801 ctgcccggga aatatcgccc agccagacat ttttaccgtt cagcgcgctg tcgttcggga
    37861 tggaatcacc gagaacaatc gccatttcag cgtcgttcgg attgtcgatg atttccagtt
    37921 ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt gcctgaccga
    37981 gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca gttaaaaggt
    38041 tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat acccacattg
    38101 ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg ttcactggat
    38161 tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc gccaacggtg
    38221 cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt aacccaaagc
    38281 gcgccttcgg cacccagtga aataacgaca tgcgcgatac cttgttcacg cagcgcatgc
    38341 gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat ttccagctcg
    38401 cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa cgcttcacgg
    38461 ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca gtcggtgaac
    38521 gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc gaactgaccg
    38581 agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgggggtaac gtcaaaaccc
    38641 gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt aattcgggtg
    38701 cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag ttgctgaaaa
    38761 ccatcctgat tgtctttacc gaggaagcca ccaacggtga catcaattcc caggtctttt
    38821 aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt tttcaccagg
    38881 ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc cggattaagg
    38941 gtgatagtag caacacgtct gctcattatg cgccctcccc aagaccagca gcgatagcgt
    39001 cgccgattgc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag cgtaggcgat
    39061 gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt gccggtttgc
    39121 cggtaccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg gtattgacca
    39181 gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac tccgcgctta
    39241 acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt gccgcatccg
    39301 ctttcagcaa gcggtcagct ttattgtcga gtaacaaatc agcgagacgc ttaagaaccg
    39361 caatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt tcgccgtcca
    39421 catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg ccttcggcgc
    39481 tatcgctcag ccagataccc tgcccgaggt tcagcggttg ttcattgatg gctttggtga
    39541 cgaaagtggc gtcaactgcc cccgcttctt tcagacgcgc agcgttcagc gcctgaagag
    39601 tcagcagatc gctggcgacg atatccagcg tcagcatttc gttgtcgagc ttcagctgct
    39661 cactctgctt ttcgcccatc agcaatgcgc gaagttcttc tgctgttgtt gctgacttca
    39721 gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc aggcccagat
    39781 gttcatccga gctggcagca ataccgatcg ccacgtacgc tacctggccg tcaccccagg
    39841 tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga tcgcgggtgt
    39901 cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt tgctgttcgc
    39961 gcgccagcat gccattgacg tagccttcag ctacattacc ggcctgcacc agcgccgcag
    40021 cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg atgtcctgta
    40081 cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag cttctatgag
    40141 aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac gtttcaagag
    40201 agcataatac ttttgtttca gcacgcaagg aaagccgtaa agttatgagc aaaagtttga
    40261 tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc gttatcagca
    40321 atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt ttgattattc
    40381 gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa ctccgcgtct
    40441 tcctcttcca gtgatcgacc agcatgcata actcccccgc agtcaccagc gcgaaatcgt
    40501 ttgacctgac ctcgacggcg tttttaatcg ttgcctttct caccggtatt gcgggcgctc
    40561 tgcaaacccc gacactcagt atttttctta ccgatgaagt acatgcccgt ccggcgatgg
    40621 tgggattctt ctttaccggc agcgctgtca ttgggattct ggtgagtcag tttctcgccg
    40681 ggcgctctga taagcgcggc gatcgcaaat cgctgattgt cttttgctgc ctgttaggcg
    40741 tgctggcctg cacccttttt gcctggaatc gcaactactt tgttttgcta ttcgttggcg
    40801 tctttcttag cagctttggc tcgaccgcta acccgcaaat gtttgccctt gcccgtgaac
    40861 atgccgacaa aaccggacgt gaggcggtga tgttcagctc ttttttacgc gctcaggttt
    40921 cactggcatg ggtcattggc ccaccgctgg cttatgcctt agcgatgggt ttcagcttta
    40981 cggtaatgta tctgagcgca gcggtagcgt ttattgtttg cggcgtgacg gtgtggctgt
    41041 ttttaccgtc aatgcaaaaa gaacttccgc tggcgaccgg cacggttgaa gcgccgcgcc
    41101 gtaaccgtcg cgatacgctg ctgctgtttg tcatttgtac attgatgtgg ggctcgaaca
    41161 gcctgtacat catcaacatg ccgctattta ttatcaacga actgcatctt cccgagaaac
    41221 tggccggtgt gatgatgggg accgccgccg ggctggaaat cccgaccatg ttgattgccg
    41281 gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg cgttgctgcc gtgggtggcg
    41341 tctgttttta cgcaggaatg ctgatggcgc attcacctgt cattctgttg ggcttgcagc
    41401 tgctaaatgc tatttttatt ggcattctgg gtggcatcgg gatgctctat tttcaggatt
    41461 tgatgcccgg tcaggcaggt tcagccacca cgctctatac caatacgtcg cgcgtgggct
    41521 ggatcatcgc aggatcagtg gcgggcatcg tcgccgagat ctggaattat cacgctgtgt
    41581 tctggtttgc gatggtgatg attatcgcca ctctgttttg cttactgcgg attaaagatg
    41641 tttaaggcgc ggtcagcatt tccagatcga taagccaggt cattgcctgc tgacgcgagt
    41701 taccgcacat ttcagcgctg gcttgtaagc ctgcacagac tttcggccgc agtggcgagg
    41761 tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca gggcgtattg gcgggcttgc
    41821 catctggcat accgggaata gggctggaaa tcgaaggggc agtgcaacac gccccgcaac
    41881 ccggacggca ttccatacgt tgttctctta aggttaacca ccggattgat gagcgcgcac
    41941 agtaccacct tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa ggcccgtcgc
    42001 gagtactttg tcgcgatatt tttgacattt tcgactacag gaatttttcg atgccaagag
    42061 cgaacgaaat taaaaaaggt atggtactga attacaacgg caaactgctg ctggttaagg
    42121 acattgatat tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa atgcgttttt
    42181 ctgatgtccg taccgggctg aaagtagaag agcgtttcaa aggagatgat atcgttgaca
    42241 ccgtgacgct gacccgccgt tacgttgact tctcctatgt cgatggcaac gaatatgtct
    42301 ttatggataa agaagactat accccgtata ccttcaccaa agatcagatt gaagaagagt
    42361 tgctgtttat gccagagggc ggcatgccgg acatgcaggt gctgacctgg gacggtcaac
    42421 tgctggcgct tgagttgccg cagaccgttg atctggaaat cgttgaaacc gcaccaggca
    42481 tcaaaggggc atctgccagc gcccgtaaca aaccggcgac attgagcact ggtctggtga
    42541 ttcaggtacc agaatactta agcccgggcg aaaaaattcg tatccatatc gaagaacgcc
    42601 gttatatggg gcgtgctgac taacttcagc cgcatgcaga aaagggatag ctcaggctgt
    42661 ccctttttta atttattata ccaatcttct attttgcgct tcctgaacac cttacgcccg
    42721 caattttcac tcgttgattg atcgccctca cacttcatcg cattaacaat ccagaccaat
    42781 ttcaattgct gtcatataac tttacactgt cattgttaat taatcgttac taagacgtga
    42841 ctcctatgaa tacaatcgcc tccgttacgc tcccgcatca tgtacacgct ccacgctacg
    42901 atcgccagca gttgcaatca cgtatcgttc attttggctt tggagccttt caccgcgcac
    42961 atcaggcgtt actgaccgat cgtgtgctga atgcccagag cggcgactgg gggatctgtg
    43021 aaatcagctt gttcagcggt gatcaactga tgagccagct ccgcgcacag aaccatttat
    43081 acaccgtgct ggagaaaggg gcggacggca atcaggcgat aattgtcggt gccgttcacg
    43141 aatgcctgaa tgcaaaactg gattccttag cggcaattat tgagaaattt tgcgagccac
    43201 aggtggcaat tgtttccctg acgattaccg aaaaaggcta ttgtattgac ccggccaccg
    43261 gtgcactcga caccagcaat ccgcggatta ttcacgatct acaaacccct gaagaacctc
    43321 actccgcacc gggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc ggccttacac
    43381 cgtttaccgt gctctcctgc gacaatattc ccgacaatgg ccatgtggtg aaaaacgcgg
    43441 tgctgggaat ggcagaaaaa cgttcgccag aactcgccgg gtggataaaa gagcacgtca
    43501 gttttccggg aactatggtc gaccgcattg ttccggcagc aaccgacgaa tcactggcgg
    43561 aaatcagcca gcatctgggg gtaaatgatc cctgcgcgat tagctgcgaa ccgtttatcc
    43621 agtgggtggt ggaagataac ttcgtcgctg ggcgtcctgc ctgggaagtc gcaggtgtac
    43681 aaatggtgaa tgatgtcctg ccatgagaag agatgaaact gcgtatgctt aatggcagcc
    43741 actcttttct cgcttatctg ggttacctct caggattcgc ccatatcagt gattgcatgc
    43801 aggatcgcgc atttcgccat gccgccagag cattaatgct ggatgagcaa gcgccgacac
    43861 tgcgaattaa agatgtcgat ttaacacaat atgcggataa gttaattgca cgttttgcta
    43921 acccggcgct gaaacataag acctggcaaa tcgcaatgga tggtagccag aaattaccgc
    43981 aacgtatgct ggcaggtatt cgcatacatc tggcgcgcga aacggactgg tcgttgctgg
    44041 cattaggcgt tgcaggctgg atgcgttacg tcagcggcgt tgatgatgcc ggaaatacca
    44101 ttgatgttcg cgatccgctt agcgataaaa ttcgcgatct tgttgcgggc agcagcagtg
    44161 agcaacgcgt caccgccctg ctttccctgc gtgaagtttt cggtgatgat ctgccagata
    44221 acccgcattt tgtgcaggcc atcgaacaag cctggcaaca aatcgcacaa ttcggcgcgc
    44281 atcaggcgct attaaacacc ctcaaaattt aacgatttct gcggttaaag cggatgaagc
    44341 tcgccttcgt ccgctctccc cttctctttt ctgccgtttt tagccaggat taacgctcag
    44401 ttaactttta agagtattgc ggagccgttg tgaccaggac caacctcatc accggttttc
    44461 tcggcagcgg gaaaaccacg tcgattcttc atctgttagc ccataaagat cccaacgaaa
    44521 aatgggcggt actggttaat gaatttgggg aagtcggaat tgatggtgct ttgctcgccg
    44581 atagcggcgc attgctgaaa gagatccccg gcggctgcat gtgctgcgtt aatggtttac
    44641 ccatgcaggt aggattgaat accttactgc gtcagggaaa accagaccgc ttgttgatag
    44701 agccgaccgg actgggtcat ccgaaacaga tcctagatct cttaaccgcg cccgtgtatg
    44761 aaccgtggat agatctgcgc gccacgttgt gcattctcga tccacgcctg ctgctggacg
    44821 aaaaaagcgc cagcaatgaa aacttccgtg accagctggc tgccgcagac attattgtcg
    44881 ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc gctacagcgc tggtggcagc
    44941 aaaatggtgg cgatcggcaa ttaattcaca gcgagcatgg gaaaattgac ggtcatcttc
    45001 tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat tctcatcagc
    45061 atggcgtgaa aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc tggcgtcgca
    45121 gtctgaacag cgggcaagga tatcaggcct gtggctggat attcgacgct gatacggtat
    45181 tcgacaccat tggcattctg gaatgggcgc gacttgcacc ggtggaacgc gtcaaaggcg
    45241 tgatgcgtat tcccgaaggg ctggtgcgaa tcaaccgtca gggcgatgac ctgcacattg
    45301 aaacgcaaaa cgttgcgcca ccggacagtc gtattgagct gatttccagc agcgaagctg
    45361 actggaatgc cttgcagagc gcgctgttga agcttcgttt agcgaccacc gcgtaaggtt
    45421 gcctgcgttt ttcagtaaga taattagaga aaatatgatt aaaaatttgc cgcaaatagt
    45481 gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg tatattcccg ttaatcatgg
    45541 attctggttg ccgattgatg cggatatttt ttattttttt aatcagaaac tggtcgaaag
    45601 taaggccttt ttgtggctgg ttgcattgac caacaatcgc gctttcgacg gttgttcact
    45661 gctggcgatg ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc caggcagacg
    45721 acgtatcgtg attattggtc tggtcatgct attaactgca gtggtattaa accagctggg
    45781 tcaggcatta attcctgtaa aacgggccag cccaacattg acttttaccg atattaaccg
    45841 cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca cgagatagct ttcccggcga
    45901 tcacggtatg atgctgctta ttttttcggc attcatgtgg cgttatttcg gcaaagttgc
    45961 aggccttatc gcccttatta tttttgtggt ttttgcgttt cccagagtaa tgattggcgc
    46021 acactggttt actgacatca ttgtcggttc gatgaccgtg atattgatcg gtttgccctg
    46081 ggtgttgctg acgccattaa gtgatcgatt aatcaccttt tttgacaaat cactaccggg
    46141 aaaaaacaaa catttccaaa acaaataact cacagtaatt aacatcatca gggttatttt
    46201 tatagtgagg ataatcctga tgatgcgcac cgtgctttca tccatcgcgc gcaaaaatca
    46261 ttctctaagt aaatgaatgg attgcatgcg tttcactcaa tcgtacttta attgaccaat
    46321 cccgtttatt aactttctgt atcacttttt cttataaaaa atcatgtaaa accgctcgcc
    46381 aagaccgcac caatcgggta atctcgaact cgttttgcct cggcggtaga ttatcctcac
    46441 agcatataat tttgtgcgtt agtccacaga tttggcctta aggaattgtt tcaacatgcc
    46501 caggtaatta gtctcgtgtc gcttggcatt tttttataac gatatttgtc gttaaggact
    46561 tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg
    46621 gatccccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata acaccgcaaa
    46681 gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc aagcttctca
    46741 ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta
    46801 tgctgactgg aaaggtgtac gctatcgtct gggtggcagc actaaaaaag gcatcgattg
    46861 ttctggtttc gtacagcgta cattccgtga gcaatttggc ttagaacttc cgcgttcgac
    46921 ttacgaacag caggaaatgg gtaaatctgt ttcccgtagt aatttgcgta cgggtgattt
    46981 agttctgttc cgtgccggtt caacaggacg ccatgtcggt atttatatcg gcaacaatca
    47041 gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg aaccgtactg
    47101 gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa ccgtttggat
    47161 gcaatccctt ggctatcctg acgagttaac tgaaagcact gcttaggcag tgcttttttg
    47221 ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata gtccggtcat
    47281 tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt tcatacgcgc
    47341 tcccaattct ggacgtaagc tcctgcttac ctgcattgtt gcaggcgtga tgattgcgat
    47401 actggtgagc tgccttcagt ttttagtggc ctggcataag cacgaagtca aatacgacac
    47461 actgattacc gacgtacaaa agtatctcga tacctatttt accgacctga aatccactac
    47521 tgaccggctc cagccgctga ccttagatac ctgccagcag gctaaccccg aactgactgc
    47581 ccgcgcggcg tttagcatga atgtccgtac gtttgtgctg gtgaaagata aaaaaacatt
    47641 ctgttcatct gcgactggtg agatggacat tccactaaaa gaattgattc cggcgctcga
    47701 cattaataaa aatgtcgata tggcgatctt acccggtacg ccgatggtgc cgaacaaacc
    47761 cgcaatcgtc atctggtatc gcaacccgtt gttgaaaaat agtggcgtgt ttgccgcgct
    47821 gaatctcaat ctgacgcctt ctctctttta tagttcacgg caggaagatt acgatggcgt
    47881 cgccctcatt attggtaata ctgcgctatc taccttttct tcacgtttga tgaacgttaa
    47941 tgaattaacc gacatgccgg tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg
    48001 gctttatgca gatgactgga catggaacga tgtgtggtac gcatttttac tgggtggcat
    48061 gagtggaact ttcgttggac ttctctgcta ttacctgatg agtgtgcgta tgcgcccagg
    48121 cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggtgtatc aaccggtggt
    48181 ggatacacaa gctttgcggg taacgggcct ggaagtactg ctacgctggc ggcatccagt
    48241 agcaggagaa atccccccgg atgccttcat taactttgcc gaagcgcaaa agatgattgt
    48301 gccactgact cagcacctgt ttgagttgat tgcccgcgat gccgcagaat tagaaaaagt
    48361 actgccggta ggcgtcaaat ttggcattaa cattgcgccg gcccacttgc acagcgaaag
    48421 ctttaaagcg gatatccaga aactgctcac ttccctgccc gcacaccatt tccagattgt
    48481 gctggaaatt accgagcgcg atatgctgaa agagcgagaa gccacacaac tcttcgcctg
    48541 gctgcattcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc acagcgcgct
    48601 tatctatctt gagcgtttta cgctcgatta tctgaaaatt gatcgtggat ttatcaacgc
    48661 catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc tggcgaaacg
    48721 cctcaatatg ctgacggttg ctgaaggggt cgaaacgcca gaacaggcac gatggctaag
    48781 cgaacgcggc gttaatttca tgcaaggcta ctggattagt cgcccgttac cgctggacga
    48841 ttttgttcgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct
    48901 tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag gaaatccgtt
    48961 gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg gtgcgcaggc
    49021 gcagactatc aaggaaagct atgcctttgc cgtactgggc gaaccccggt acgcatttaa
    49081 tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaaa taacgttgtc
    49141 tgcgctcggc accttcgata atttcaaccg ctatgcactg cgcggcaatg cgggcgcacg
    49201 caccgagcag ctgtacgaca cgctatttac gacttccgat gacgaaccag gcagttatta
    49261 cccgctgatt gctgaaagcg cacgctatgc tgacgattat tcctgggtgg aggtcgctat
    49321 taatccacgc gcccgttttc atgatggttc gcccattact gcccgcgatg tagagtttac
    49381 ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac
    49441 cgtcaaagcc attgcgccgt taaccgtgcg cattgagtta gcgaaacccg gcaaagaaga
    49501 tatgctgagc ctgttttcgc tgccggtctt tccagaaaag tactggaagg atcacaaact
    49561 tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg taccgaatta cgtcctggaa
    49621 aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa acttaccggt
    49681 aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg atgataatgt
    49741 cgcctttgaa gcgtttaaag ccggtgcctt tgatttgcgt ttggaaaacg acgccaaaaa
    49801 ctgggtcacg cgctataccg gtaaaaattt cgataagaaa tacatcatca aagatgagca
    49861 aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac gtccggtatt
    49921 cagcgatcgc cgggtgcggg aagcaatcac tctcgccttt gactttgaat ggatgaacaa
    49981 ggcgttgttt tataatgcct ggagtcgcac gaacagttat tttcagaata ccgaatatgc
    50041 ggccagaaat taccccgacg ccgcggagct ggtgcttctg gcaccaatga aaaaagatct
    50101 accgccagaa gtcttcacgc aaatctacca gccgccggta tccaaaggcg atggctacga
    50161 tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa gcgggctggg tgctgaaggg
    50221 tcagcaacgc gtaaatgcca caacgggtca gccgctcagc tttgagttat tgctaccttc
    50281 gagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa
    50341 catggacatt cgcaaagtgg ataactcgca aatcaccaac cgcatgcgca gtcgcgacta
    50401 tgacatgatg ccgcgcgtat ggcgggcgat gccgtggccc agttccgatt tacagatttc
    50461 ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa gcccggttat
    50521 cgactcgctg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat tactgccgtt
    50581 ggggcgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa tgtggtacat
    50641 ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtac gccctgttta
    50701 cagcctgggt attgatacct ggtggtatga cgttaataaa gcgacgaaac tgccgtcagc
    50761 cagacaacag ggagagtaga tgggcgctta tctgattcgc cgtctgttgc tggtgatccc
    50821 aacgttatgg gcgattatca ctatcaactt tttcatcgtg caaattgcgc ctggcggtcc
    50881 ggtcgaccag gccatcgccg ccattgagtt tggtaatact ggtgtattac ccggtgcagg
    50941 cggtgaaggt gttcgcgcca gccatgcaca aacgggcgtc ggtaatatca gcgacagtaa
    51001 ttaccgtggc ggacgcggat tggatccaga agtgatcgcc gagatcactc atcgctacgg
    51061 tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca tccgttttga
    51121 ttttggcgac agcctttttc gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc
    51181 ggtctccatc accctcggat tgtggagcac gctgattatc tatctggtgt cgattccgtt
    51241 aggcattcgc aaagctgttt ataatgggag ccgttttgac gtctggagta gtgcatttat
    51301 catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg tcttcttcgc
    51361 gggcggcagc tatttcgacc tgttccctct gcgcggcctg gtttccgcta actttgattc
    51421 gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctgc cggtgctggc
    51481 gacagtgatt ggcggctttg cggctctgac catgctgaca aaaaactcat tccttgatga
    51541 agtgcgtaag caatacgtgg tgacggcacg tgcgaaaggg gtaagtgaaa aaaatattct
    51601 ctggaaacat gtgttccgca acgccatgct gctggtgatt gccggttttc cggcgacgtt
    51661 tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac tcaatggtct
    51721 tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt
    51781 gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca gctatacgct
    51841 ggtcgatccg cgtatagatt ttgagggacg ttaatgtcgc gactcagccc cgtcaatcag
    51901 gcccgttggg cgcgttttcg tcataaccgt cgcggctact ggtcgttatg gattttcctc
    51961 gtcttgtttg gtttgagttt gtgttctgaa cttatcgcca acgataaacc gttgctggtg
    52021 cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag cgattttggc
    52081 ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg gctggaaaat
    52141 aacggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa ctttgctacc
    52201 gataagccct tcccttctcc accctcccgg caaaactggc tgggaacgga tgccaatggc
    52261 ggcgatgtgc tggcgcgcat tctctatggc acgcggatct cggttctgtt tggcctgatg
    52321 ctgactctct gttccagcgt gatgggcgtg ctggcggggg cgctacaagg ctattacggc
    52381 ggtaaagtcg atctctgggg gcaacgcttt attgaagtat ggtcagggat gccgacgctg
    52441 tttttgatta ttttactttc cagcgtcgta cagcctaact tctggtggct gctggcaatt
    52501 accgtcttgt ttggctggat gagtctggtc ggcgtggtgc gggcggagtt tttacgtacc
    52561 cgtaatttcg actacatccg cgcggcgcag gcgcttggcg tcagcgatcg cagtatcatc
    52621 ctgcgtcata tgttgccaaa tgccatggtc gctaccctca cctttttacc gtttatttta
    52681 tgtagttcga taaccaccct gacctcactc gatttcctcg gcttcggtct gccgctcggt
    52741 tcaccgtcac tcggcgaact gctgttacaa gggaaaaata accttcaggc cccgtggctt
    52801 gggatcaccg ccttcttgtc ggtggcgata ttgttgtctt tgctgatctt tattggtgaa
    52861 gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa ctctgttagc
    52921 gattgaaaat ttgtcggtgg gttttcgcca tcagcaaacc gtacgcacag tagtcaatga
    52981 tgtttcactt cagattgagg ctggcgaaac gctggcgctg gtgggtgagt caggttcagg
    53041 caaaagcgtt accgcgctgt caattttacg cctgctccct tccccgccgg ttgaatatct
    53101 ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgatc aaacgttgcg
    53161 cggtgtacgc ggtaataaga tcgccatgat ttttcaggag ccgatggtgt cgttaaatcc
    53221 attacatacc ctggaaaaac agctttatga agtactttca ctccaccgcg ggatgcgtcg
    53281 ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc gccaggcggc
    53341 aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc gggtgatgat
    53401 tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccaa ccaccgcgct
    53461 ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag gcgagctgaa
    53521 tatgggtatg ctgtttatta ctcataacct cagcattgtt agaaaactgg cccaccgcgt
    53581 ggcggtaatg caaaacggtc gctgtgtcga gcaaaataac gccgctacgc tatttgcctc
    53641 acccactcat ccttacacac aaaagctact caacagtgaa ccgtcaggcg acccggtccc
    53701 attgccagaa cctgcctcaa cgttgctgga tgttgaacag cttcaggttg ccttccccat
    53761 tcgcaaaggg attttgaagc gcattgtgga tcataatgtg gtggtgaaaa acatcagttt
    53821 tacgctacga gcgggtgaaa cactgggttt agtgggcgag tctggttccg ggaaaagcac
    53881 gacgggactg gcgctgctgc gactgattaa ctctcagggc agcatcgtct ttgacggtaa
    53941 gccactgcaa aatttaaatc gccgccagct gttacctatt cgtcatcgca ttcaggtggt
    54001 atttcaggat ccaaactcct cgctcaaccc acgactcaac gttttgcaga ttattgagga
    54061 aggcttacgg gttcaccagc cgacgctttc tgccgcgcaa cgcgaacaac aagtgatagc
    54121 cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg cggagttctc
    54181 tggtggtcag cggcaacgta ttgcgattgc cagggcgtta attcttaagc cctcgctgat
    54241 cattcttgat gaaccaacat catcactcga caaaacggtg caggcgcaaa tattgacgct
    54301 attgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc acgatttgca
    54361 cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag tagtggaaca
    54421 aggaccgtgc gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc agctactggc
    54481 gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa
    54541 aatttttcaa tcggcatgtt gcagcaaatt cgtcctggcg atttacaaac aggcacggct
    54601 caccttcaca ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc tggcttagtt
    54661 tgtacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc gccaccaggc
    54721 tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag
    54781 cagaccagcg atagttctgc ggcagtcgat ggacgattga gagttgtaat gaagtcactt
    54841 attttccccg gaagtacatt ttacttcaca atttgtttac atcaatttta acacatcatc
    54901 aacaatccgt ctttatacag atcgtaaaga ttgatgcctc gcctgcgtgg cctctatggc
    54961 tctgatttaa ataatatctg cgggcgtggt cacgtttgcg gggctatatg tacccgtttc
    55021 tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact ttttacataa attgatttta
    55081 cataaaataa acatatatcg ggggaatgat agatttgtgg ttgacatgca tcaacaaagg
    55141 aagcctttta gcttcctcgt tgtgcaatag atcaccgttt tttcggccga ctggcgtaca
    55201 aacagaagag aatggagctg gttgcgcaga atgcaattga ccaaatcatc ggccatgcag
    55261 agttaaaggt cgcaagagaa agtaatgcgc caacaattgc ccctatgccg aaacggaagg
    55321 ttcctgccag cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga atgaccgcca
    55381 tcgcattgga tgacaccatc gacacgcagc ccacaaacgc cgcaacgcca accaccagcg
    55441 accagaatcc cagccccagc agtgcactga tgaccatcca cgctgccata ataaactgta
    55501 tccacaaccc cgagcggaac atatttaacg cgccaatacg gcggacgaag cggctgttaa
    55561 agatggtcat cacgaacaga aaaagaatgt ttagcgcaaa gtaataacca aagttttccg
    55621 gcgcgacgtg gttgatttca atgtagacaa acggtccggc gcttaagaat gagaacatcc
    55681 cggcaaagct gaaaccgctg gccagcatat aactcaggac acgcttatga cggaacagcg
    55741 ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg gcgacgctcc ggtggtaagg
    55801 tttctttgat cagaaagaag atcattgccg aagccagaat cgccgccacg gcgaggatcc
    55861 agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc ggtgccatca
    55921 gcggtgcaat ggtcgtcacc agcatgacaa acgacatcat ccgtgagaac tcttctttcg
    55981 ggtagatatc acgcatcaaa gcgttgataa ccacgctggc cgcagccgca gccagcccgt
    56041 ggaagaaacg catcacaatc agctgatcga tggtttgtgc caacgcacac gccaccgcgg
    56101 cggcggcaaa caccagcgta ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca
    56161 tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactg agggtcatct
    56221 gcgtactgcc cgccggtaca ccaaactgag ctgaaattac cggtagcgca ggcagataca
    56281 tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg
    56341 acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt tagacgacgc
    56401 tggcaatttc ttcttcagtt aacggacgat attcaccggg agctaaatca gcatccagcg
    56461 taatgccgcc aatacgttca cgatgcagct caaccacatg gttacccacg gcggcgaaca
    56521 tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa
    56581 tcacttccag caccgccggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag
    56641 caaattgctc tgccgtatcg tcagctacgg gtgattccag cgtcaccaga taggtcttct
    56701 cgcaatgatg gcgcggggaa gtaatgcggt gcgaccactg accatcatct gtcattagta
    56761 ccagaccggt ggtatcgata tccagacgcc ctgcagcgtg cagtttccac gctaccggtt
    56821 catcaagaaa atagagcacc gttgggtgat ccgggtcgtc cgtggagcaa acatagccct
    56881 gaggcttatt gagcatgaag taacgtggac cgtgttgctg cgccagcggg ttgccatcgt
    56941 aagcgacatc atgttcaggg agcagtttga acgctgcatt acggacgatt tcgccatcga
    57001 cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct
    57061 gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaaac ggaggtcggg
    57121 taatgacccg aaaatctgaa ctgttgtctg cccagtatag cggtctaaca acatccctca
    57181 agggaaaaag atccatggca tactattagc agaataatct acctacgcga gaccatgatt
    57241 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    57301 cataaaaccc ctgccgttat cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg
    57361 gaactggcgc ggctggcgcg tggtcgcgtg ctggtgctgg cacacgttaa agaactggtg
    57421 gcgcaaaacc atgcaaaata tcaggcgctg gggctggaag ccgatatttt tgccgccggg
    57481 ctaaagcgca aagagagcca cggtaaagtg gtatttggca gcgtgcagtc ggtcgcccgt
    57541 aatcttgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt
    57601 ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgaccaa agtgaatccc
    57661 cacttacgcc tgctggggct gactgccacg ccttttcgac tgggcaaagg ctggatttat
    57721 cagttccatt atcacggcat ggtacgcggc gatgagaaag cccttttccg tgactgcatt
    57781 tatgagctac cgctgcgtta tatgattaaa cacggctatc tgacgccgcc agaacgactg
    57841 gatatgccag tagtgcaata cgatttcagc cgcttgcagg cacagagtaa cgggctattc
    57901 agcgaagccg atctcaaccg cgagctgaaa aaacagcaac gcattacccc gcacatcatc
    57961 agccagatta tggagtttgc tgtaacgcgc aaaggggtga tgattttcgc cgccaccgtt
    58021 gaacacgcaa aagagattgt gggactgctg cctgccgaag atgctgcact gattactggc
    58081 gacacccccg gcgctgagcg cgatgtgtta attgaagatt ttaaagccca gcgttttcgc
    58141 tatctggtca acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    58201 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    58261 cgtctcgctc ccggcaagac tgattgctta attcttgatt atgcgggtaa ccctcacgat
    58321 ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt
    58381 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    58441 ttgattgaac actttggtcg tcgctgtcag ggatggtttg aagatgacga cggacatcgc
    58501 gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc tgaaaatgat
    58561 attgccgccc gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta
    58621 aaagcggcgc tacgactgaa agacgcgctg gtattacgct gtagcggcat gtctttgcag
    58681 catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggcgcg
    58741 gatgtgagtg agcgtttccg tctgcaaacg cccgcccagc gtaccgcctt cgaacaactt
    58801 tttatccgtc cgcatacgcg cacaccgggc atccctctgc gctggatcac cgccgccgat
    58861 atcctcgccc agcaagcatt actgcgacac ccggattttg tcgtcgcccg catgaaaggt
    58921 cagtactggc aagtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac
    58981 gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta tagaatctcg
    59041 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    59101 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    59161 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    59221 agaagcgccg ctggctgtcg aactggatca cgacaaagtc atgaacatgc aagttaaagc
    59281 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    59341 tcaggacgta cagcgtcacc cgtacaaacc gaagctgctg cacatcgact tcgttcgcgc
    59401 ttaattgctg aataagttgt aaaaaacccc gctccggcgg ggttttttgt ctctgcaaat
    59461 tatgcctgat tacggtattg ctattttttg caggccagat aaggcgttca cgccgcatct
    59521 ggcattcaga acacaaaacc gattaattgc cgccagacgt ccggcgctgc aattgatcgc
    59581 gcaagttcgg cggcgtgcct ttaatggtca gggtatcggt tgctggatcc cagaagatgc
    59641 gttcacctaa cagcatcgca tcaaagttaa tagtcaagcc accgccgcta ccggcaaact
    59701 tcgtcaactg acgcagtgtg ctacggtctg ccgggaagct ctcttccagt tcgtagcctt
    59761 tttcagccgc aaactccgtg aagctcactt cgctgacgcc tgccagctct ttcgacagcg
    59821 attccagttc aatctcttca cctgcctgca gctgctcatt acagtagcta taaacctgct
    59881 ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcgaccg
    59941 cttgcaacag cccacggttt tgcgctttcg cgtttaaccc ttcgctggca ccgaggaaat
    60001 ccataaagaa atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg
    60061 tcgactctgg attggtttcc cactcggtta aatcgatacg cgcaacaata tccgcatgat
    60121 tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacacgc atactgctca
    60181 ggttgctcag aactgccacc agcagatact ccaccgccag atagcgatag tggcaaaaca
    60241 gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgaccggttg
    60301 ccgcgcggct aaatgccaga aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg
    60361 ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat
    60421 gcagttctgc caccatctca acgacggttt ccgtcggttc cagcaatgaa tcgcgcaaca
    60481 ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat
    60541 ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc
    60601 gacgcaataa aagatggcgg tcattttaca gaaggcggcg taacgagaat gatttaacgc
    60661 ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact
    60721 gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg
    60781 cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt
    60841 taggcaacat ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa
    60901 ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag
    60961 ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag
    61021 atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc
    61081 agccgttacc tgtttatcgc cgactggccg acaacgcttg ctggtcgcat ttattcctac
    61141 gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc
    61201 ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca
    61261 acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat
    61321 cttaatccca tcgtctggca actggttatc aacccagacg aaaatgagat ggcgcgcgac
    61381 tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg
    61441 tggagctggc aaaagctacg cagcctgacg cgtcgtcggc gcttcgcgcg cccgctggcc
    61501 gcattcttat ttatcgcctt tatcgcctcg catgtggttt atatctgggc cgatgccaac
    61561 ttctatcgcc cgatcaccat gcaacgcgct aacctgccgc tttcgtaccc gatgacggcg
    61621 cgacgttttc ttgagaagca tggtctgctt gatgcgcagg agtatcaacg ccgtctcatt
    61681 gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc
    61741 gatatgggca ccgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc
    61801 ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc
    61861 catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta tggcatctcg
    61921 ccgagctata tggatggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt
    61981 aatcagcaag gctatcagct ggggttattc tcgtcagatg gctttaccag cccgctgtat
    62041 cggcaggcat tgttgtcaga tttctcgatg ccgagtgtac gcacccaatc cgacgaacag
    62101 accgccacgc agtggatcaa ctggctgggc cgctacgcac aagaagataa ccgctggttc
    62161 tcgtgggtct ctttcaatgg cacaaacatt gacgacagca atcagcaggc atttgcacgg
    62221 aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg
    62281 cgtgattctg gcaaactgga caatacggtg gtgattatca ctgccgggcg gggtattccg
    62341 ctgagcgaag aggaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg
    62401 attcactggc caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg
    62461 atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa
    62521 ggtcaggatt tgttcaaccc tcaacgccgc cattactggg ttaccgccgc ggataacgat
    62581 acgctggcaa ttaccacccc gaaaaagaca ctggtgctga acaataacgg taaataccgc
    62641 acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg
    62701 caagtactga cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagca
    62761 aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt agcagccacg agtcggcacg
    62821 tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc
    62881 gtgccgacca aaaatcccaa gaaaaccaac ctattgcggt tggttttttt atatctgcat
    62941 ttaattcgat aaacagacag cgacacatca cgacctgttt attttctgtt atcagaacgt
    63001 ccagactaca cccgcctgag ttgtgctgct ttcttcacgg gagaaacgac tatcgatacc
    63061 gagcgtcagg cccgtcaatg tttgctcaaa gccagctccc gcatcagtgt tcacgttgtt
    63121 gcggctgtta attgccgaac tccacatcgc cgccctttac gctaccaaga cgctcacgca
    63181 cggtgtccag ttctgcatca aataccagcg gttgtgctgc cgccatattc agcacatccg
    63241 tggttgaagg gggcatttgt acgcgattct ctgtgagact ccagctatgg ttgccattat
    63301 ccagcaaggt atattcatac gtactgatat caacaacgct tccggcattg cccaacgtaa
    63361 atgccgcatc accgcgccct cctttaatcg tgggatcagt cgccgcgcca taaacctgta
    63421 gcactccatt attgatcgta gtctggttta ccgcgccacc catttgaaca atttgataac
    63481 catcatttaa tactgagtat tctgcggtgc catttatttc aatgtactga tatcccccgt
    63541 taattttagt attactactt acaccaaatt cttatatatg ctgttctccg ccaggattga
    63601 ttttcgtatt attggcggcc ccataaacca gctggatatc tttttccagg acaacaccat
    63661 caactgtctc accatactca acagttgatg ccaatgctat gggtgaatat aacgctgtag
    63721 ccaccagaac agatattgcg gaacgacaaa gagaaacaga accagattgg tgcattaagc
    63781 tttcatccta tgaaattaat tgctgttaaa agcattgggg acagaaaata cccatagctc
    63841 catacccgga gtcagtttta aaaaactgtt taaagaaatg cacaagtatt gtgattgatt
    63901 ttttagttgt ttttcctgat gagaagctga tgcaaaattc cgtctttata atgaagatga
    63961 tgccaaagcg aacgacaaag ttgtagtttt cactacatgt ccatacataa aatggggtaa
    64021 cattcacgcg cctggtagcg ttaccaacgc tacgctcaaa cataatgatt ctaataaaac
    64081 ctcaggagac tactatgcct gaagcaacgc cttttcaggt gatgattgtg gatgatcatc
    64141 cacttatgcg acgcggtgtt cgtcagttac tggagcttga tcctggcttt gaagtggtcg
    64201 ccgaagcggg cgacggcgcg agcgcgatcg atctggcgaa tagactggat atcgacgtga
    64261 tcttgctgga tctcaatatg aaaggtatga gtggcctgga taccctcaat gccttgcgca
    64321 gggatggcgt taccgcgcaa attattatcc tgaccgtatc cgatgcctcc agcgatgtct
    64381 ttgcgctgat agacgcaggc gcagacggtt atctgttgaa agacagcgac ccggaagtgt
    64441 tgcttgaagc gattcgtgcc ggagcgaaag gcagcaaagt ttttagcgaa cgcgtcaatc
    64501 agtacttacg tgaacgtgaa atgtttggcg cggaagaaga tcccttcagc gtgctgacgg
    64561 agcgcgagct ggatgttctg cacgagctgg cacaggggct gtcaaataaa cagattgcct
    64621 cggtgttgaa tatttccgag cagacagtaa aagtacacat tcgcaatctg ctgcgtaaac
    64681 tcaatgtccg ctcacgcgtg gcggccacca ttctgttcct gcaacaacgc ggggcacaat
    64741 aaaaatagcc cgatggcatc accatcgggc cactttttat ttactctcct gcggcgacaa
    64801 atgttgcatc gcctgcgcga tactacgttc gatcaccgca cggcgagtat cgttggcagg
    64861 taagagtttc aacatcattt cccacgcggc aacggcttcg ccaaatcgtt gctgctcaaa
    64921 ggcattaaat gcatacatgc ttaacacacg gatattgcta tggtccgttc tcaccaactg
    64981 gcgcagcagt tcgccgccga ggcggttgtc gttgggatca gatgaacgtg tcaacgcttc
    65041 agcgtatccg agcgccgcat cactgttctt cggatcgagg cgatatgcag tagcgtatgc
    65101 atcggtggcg atactggcgt tacccagcgc catgccaacg cggcccaaca taatccagcc
    65161 ttctatatct cccggatttt tttgcagttg agtacgcatc cccagcgcaa ggcgcgacat
    65221 ctcttcttcg ttgagcggag cggctttcgg atccagcgcc ctgtccagta acgccggagc
    65281 ctgcgcggtg gcctgctgcc agattttcac ctgctgataa ttgccagtct ggtagtagct
    65341 gacgccagcc acaattaacg ccaccacaat acccggcaga taaacaacat atccggcacg
    65401 cttaccttcc ggcacgcttt gttctggaaa cgcttccggc accacgcgta cccgacgccg
    65461 cgaacgggcg tatatgaccc aaccgccaat gccaatagcc actactggca gcacccacag
    65521 cagcacggtc agcggcgtta acggcggatc gtaagtgacg aagttgccgt aacgcgccac
    65581 catataatcg acaatctctt tcttactttt gccttcctgc atcagttcat acactttctg
    65641 gcgcaggtcg gtggcaatca tcgagttgga atcggcaatg ctgttgttct ggcatttcgg
    65701 gcagcgcagt tcttcagtga gctgacggaa ctgttgttcc tgtgcttcat ctttaaactg
    65761 caacacgtcg atggtcgcca gcgctgagcc ggagatcatc agcatcagca cgcccaataa
    65821 aaacctcatt gtgcggcctc cttactgtat ttctcccaca gtggcttgat ctcttcttcc
    65881 cagacgcgag gattgagatc gcccgcatgg cgatagcgaa tgatgccgtt gccgtcaata
    65941 aggaacgttt ctggcgcgcc atagacaccg agatccagcc ctaacatgcc gtcgccatca
    66001 aacaggctta gtgcgtaagg attgcccaac tctttcagcc agctgattgc cttctggcga
    66061 tcgtctttat agttcatgcc gaccacgcgg atgccctgcg cagaaagctg attcagatat
    66121 tgatgttccg cacggcaggt cggacaccag gtcgcccaga cgttaagcag caccggtttg
    66181 ccctgagtca gcacatccgc ctgataaaac tgccccggat tgtccagtga ttcgaggcga
    66241 aacttcggca ctagcttgcc aatgagcgcc gattccagat tggtcggatc atccccttcg
    66301 gcattacgcg ccagctgcca cagtagcgcc gcggcaatcg ccaggaagat aatcaacgga
    66361 attaacaata ctttgcgctt catacagcct ccggcgcagt tttctgcggg ttcacgcgct
    66421 tacgatagcg aggatcaaac agg
//
LOCUS       DAZGTJ010000015        64014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000015 DAZGTJ010000000
VERSION     DAZGTJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.015"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(22..282)
                     /gene="yfhL"
                     /locus_tag="ACHK8E_001284"
     CDS             complement(22..282)
                     /gene="yfhL"
                     /locus_tag="ACHK8E_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP0343575.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(338..1186)
                     /locus_tag="ACHK8E_001285"
     CDS             complement(338..1186)
                     /locus_tag="ACHK8E_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0343576.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1395..2030
                     /gene="yfhb"
                     /locus_tag="ACHK8E_001286"
     CDS             1395..2030
                     /gene="yfhb"
                     /locus_tag="ACHK8E_001286"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP0343577.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2088..2591
                     /gene="tadA"
                     /locus_tag="ACHK8E_001287"
     CDS             2088..2591
                     /gene="tadA"
                     /locus_tag="ACHK8E_001287"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP0343578.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2588..4144)
                     /gene="mltF"
                     /locus_tag="ACHK8E_001288"
     CDS             complement(2588..4144)
                     /gene="mltF"
                     /locus_tag="ACHK8E_001288"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP0343579.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4402..8289
                     /gene="purL"
                     /locus_tag="ACHK8E_001289"
                     /gene_synonym="purI"
     CDS             4402..8289
                     /gene="purL"
                     /locus_tag="ACHK8E_001289"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP0343580.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8846..10273
                     /gene="qseE"
                     /locus_tag="ACHK8E_001290"
     CDS             8846..10273
                     /gene="qseE"
                     /locus_tag="ACHK8E_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP0343581.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10438..11151
                     /gene="qseG"
                     /locus_tag="ACHK8E_001291"
     CDS             10438..11151
                     /gene="qseG"
                     /locus_tag="ACHK8E_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP0343582.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11141..12475
                     /gene="glrR"
                     /locus_tag="ACHK8E_001292"
     CDS             11141..12475
                     /gene="glrR"
                     /locus_tag="ACHK8E_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP0343583.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12536..12874
                     /gene="glnB"
                     /locus_tag="ACHK8E_001293"
     CDS             12536..12874
                     /gene="glnB"
                     /locus_tag="ACHK8E_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP0343584.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(12919..14109)
                     /gene="hmpA"
                     /locus_tag="ACHK8E_001294"
     CDS             complement(12919..14109)
                     /gene="hmpA"
                     /locus_tag="ACHK8E_001294"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP0343585.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14438..15691
                     /gene="glyA"
                     /locus_tag="ACHK8E_001295"
     CDS             14438..15691
                     /gene="glyA"
                     /locus_tag="ACHK8E_001295"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP0343586.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15754..16947)
                     /locus_tag="ACHK8E_001296"
     CDS             complement(15754..16947)
                     /locus_tag="ACHK8E_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0343587.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17065..20346
                     /locus_tag="ACHK8E_001297"
     CDS             17065..20346
                     /locus_tag="ACHK8E_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP0343588.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20443..21426
                     /locus_tag="ACHK8E_001298"
     CDS             20443..21426
                     /locus_tag="ACHK8E_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0343589.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21449..22960
                     /locus_tag="ACHK8E_001299"
     CDS             21449..22960
                     /locus_tag="ACHK8E_001299"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0343590.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            22985..23983
                     /locus_tag="ACHK8E_001300"
     CDS             22985..23983
                     /locus_tag="ACHK8E_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0343591.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24016..25110
                     /locus_tag="ACHK8E_001301"
     CDS             24016..25110
                     /locus_tag="ACHK8E_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0343592.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25122..25994
                     /locus_tag="ACHK8E_001302"
     CDS             25122..25994
                     /locus_tag="ACHK8E_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0343593.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26143..26565)
                     /locus_tag="ACHK8E_001303"
     CDS             complement(26143..26565)
                     /locus_tag="ACHK8E_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0343594.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26662..27864)
                     /gene="hcaD"
                     /locus_tag="ACHK8E_001304"
     CDS             complement(26662..27864)
                     /gene="hcaD"
                     /locus_tag="ACHK8E_001304"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP0343595.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(27874..28686)
                     /gene="hcaB"
                     /locus_tag="ACHK8E_001305"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27874..28686)
                     /gene="hcaB"
                     /locus_tag="ACHK8E_001305"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP0343596.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28683..29003)
                     /locus_tag="ACHK8E_001306"
     CDS             complement(28683..29003)
                     /locus_tag="ACHK8E_001306"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP0343597.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(29003..29521)
                     /gene="hcaF"
                     /locus_tag="ACHK8E_001307"
     CDS             complement(29003..29521)
                     /gene="hcaF"
                     /locus_tag="ACHK8E_001307"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP0343598.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29518..30879)
                     /gene="hcaE"
                     /locus_tag="ACHK8E_001308"
     CDS             complement(29518..30879)
                     /gene="hcaE"
                     /locus_tag="ACHK8E_001308"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP0343599.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31015..31905
                     /gene="hcaR"
                     /locus_tag="ACHK8E_001309"
     CDS             31015..31905
                     /gene="hcaR"
                     /locus_tag="ACHK8E_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP0343600.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32065..33204
                     /locus_tag="ACHK8E_001310"
     CDS             32065..33204
                     /locus_tag="ACHK8E_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP0343601.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33196..34476)
                     /gene="csiE"
                     /locus_tag="ACHK8E_001311"
     CDS             complement(33196..34476)
                     /gene="csiE"
                     /locus_tag="ACHK8E_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP0343602.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34667..35521)
                     /locus_tag="ACHK8E_001312"
     CDS             complement(34667..35521)
                     /locus_tag="ACHK8E_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0343603.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35666..36469)
                     /gene="suhB"
                     /locus_tag="ACHK8E_001313"
     CDS             complement(35666..36469)
                     /gene="suhB"
                     /locus_tag="ACHK8E_001313"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP0343604.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36588..37328
                     /gene="trmJ"
                     /locus_tag="ACHK8E_001314"
     CDS             36588..37328
                     /gene="trmJ"
                     /locus_tag="ACHK8E_001314"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP0343605.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37598..38086
                     /gene="iscR"
                     /locus_tag="ACHK8E_001315"
     CDS             37598..38086
                     /gene="iscR"
                     /locus_tag="ACHK8E_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP0343606.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38198..39412
                     /gene="iscS"
                     /locus_tag="ACHK8E_001316"
     CDS             38198..39412
                     /gene="iscS"
                     /locus_tag="ACHK8E_001316"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP0343607.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39440..39826
                     /gene="iscU"
                     /locus_tag="ACHK8E_001317"
     CDS             39440..39826
                     /gene="iscU"
                     /locus_tag="ACHK8E_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP0343608.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39843..40166
                     /gene="iscA"
                     /locus_tag="ACHK8E_001318"
     CDS             39843..40166
                     /gene="iscA"
                     /locus_tag="ACHK8E_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP0343609.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40262..40777
                     /gene="hscB"
                     /locus_tag="ACHK8E_001319"
     CDS             40262..40777
                     /gene="hscB"
                     /locus_tag="ACHK8E_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP0343610.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40794..42644
                     /gene="hscA"
                     /locus_tag="ACHK8E_001320"
     CDS             40794..42644
                     /gene="hscA"
                     /locus_tag="ACHK8E_001320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP0343611.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42646..42981
                     /gene="fdx"
                     /locus_tag="ACHK8E_001321"
     CDS             42646..42981
                     /gene="fdx"
                     /locus_tag="ACHK8E_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP0343612.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            42993..43193
                     /gene="iscX"
                     /locus_tag="ACHK8E_001322"
     CDS             42993..43193
                     /gene="iscX"
                     /locus_tag="ACHK8E_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP0343613.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43252..44535
                     /gene="pepB"
                     /locus_tag="ACHK8E_001323"
     CDS             43252..44535
                     /gene="pepB"
                     /locus_tag="ACHK8E_001323"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP0343614.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44654..45430
                     /gene="sseB"
                     /locus_tag="ACHK8E_001324"
     CDS             44654..45430
                     /gene="sseB"
                     /locus_tag="ACHK8E_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP0343615.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45462..45587)
                     /gene="timP"
                     /locus_tag="ACHK8E_001325"
     CDS             complement(45462..45587)
                     /gene="timP"
                     /locus_tag="ACHK8E_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP0343616.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
     gene            complement(46249..47094)
                     /gene="sseA"
                     /locus_tag="ACHK8E_001326"
     CDS             complement(46249..47094)
                     /gene="sseA"
                     /locus_tag="ACHK8E_001326"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP0343617.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            47301..52262
                     /gene="yfhM"
                     /locus_tag="ACHK8E_001327"
     CDS             47301..52262
                     /gene="yfhM"
                     /locus_tag="ACHK8E_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP0343618.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            52263..54575
                     /gene="pbpC"
                     /locus_tag="ACHK8E_001328"
     CDS             52263..54575
                     /gene="pbpC"
                     /locus_tag="ACHK8E_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP0343619.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            54724..55155
                     /gene="ndk"
                     /locus_tag="ACHK8E_001329"
     CDS             54724..55155
                     /gene="ndk"
                     /locus_tag="ACHK8E_001329"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP0343620.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            55305..56459
                     /locus_tag="ACHK8E_001330"
     CDS             55305..56459
                     /locus_tag="ACHK8E_001330"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP0343621.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            56744..57751
                     /gene="rodZ"
                     /locus_tag="ACHK8E_001331"
     CDS             56744..57751
                     /gene="rodZ"
                     /locus_tag="ACHK8E_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP0343622.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            57778..58896
                     /gene="ispG"
                     /locus_tag="ACHK8E_001332"
                     /gene_synonym="gcpE"
     CDS             57778..58896
                     /gene="ispG"
                     /locus_tag="ACHK8E_001332"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP0343623.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            59007..60281
                     /gene="hisS"
                     /locus_tag="ACHK8E_001333"
     CDS             59007..60281
                     /gene="hisS"
                     /locus_tag="ACHK8E_001333"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP0343624.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            60299..60919
                     /gene="yfgM"
                     /locus_tag="ACHK8E_001334"
     CDS             60299..60919
                     /gene="yfgM"
                     /locus_tag="ACHK8E_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP0343625.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            60930..62108
                     /gene="bamB"
                     /locus_tag="ACHK8E_001335"
     CDS             60930..62108
                     /gene="bamB"
                     /locus_tag="ACHK8E_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP0343626.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            62226..63698
                     /gene="der"
                     /locus_tag="ACHK8E_001336"
     CDS             62226..63698
                     /gene="der"
                     /locus_tag="ACHK8E_001336"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP0343627.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            63761..63976
                     /locus_tag="ACHK8E_001337"
     CDS             63761..63976
                     /locus_tag="ACHK8E_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0343628.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
BASE COUNT        15107 a        16071 c        17722 g        15114 t
ORIGIN      
        1 ctctacatcc agggtagaat attaaatttt atccgcgtga tgcataagca caaatttatc
       61 ccacaactgt tcttccgtct cgacatgttg cggatccttc acaatagtat tggggatcgg
      121 gcacaccttc tggcaggttg gtgtctcgta gtgccctacg cattcggtac acttatcgct
      181 gttaatctcg tagatatgat cgcccattga aatcgcctca ttcgggcatt cgggttcaca
      241 catatcacaa ttgatgcatt ttttagtgat taacagcgcc ataaggaaac tctcaaaaca
      301 acacaaaccg ggcgggcatt atacgcgcat tctttactca gaccagtttt ttcaccagcg
      361 cttcactgtg acgaatacgt tctggagcca gttccagatc ctgctgaatc agcgcaatga
      421 acagcaaatc cgttaacatt ccctgcgcgt gacaagcaga gattgaagca ctgtttgtcg
      481 cttgttcttc ggcaatggta tacaggcaat gagaagcacg ctgttgcagg gcattgggag
      541 taaagccggt aatcgccagc acttttccgc ccactcgcag catctcatct gccgccaggt
      601 ttaactcgcg tcgtacaccg gtgtaggaaa tggctaataa cagatcgtca ggggacgacg
      661 cctgtactgt agcgagtagc gcatgcatat cgcgcactgc ggcagcattg aagccaatct
      721 tcatcagctt ccaggcaaag ttttgcgcca ccagacccga agcgccaata ccggtcagaa
      781 ttatccgccg cgcagagcgc aacattgcca cgcattcatg cagtttctct tcactattaa
      841 cgtttaacgt tgcgtacata gcggcagtat tttctttaat cagtttttcg ccgaccagcc
      901 gtaacggatc atcaccgcgg atttggttat gaatgggcac ggagggagat tccggctggc
      961 ttgccagcgc ttcactcaac gccaacttaa gtgccggaaa acctttatag ccgagttttt
     1021 gcgcgaactt cacgacgctg gactgactga ctccggcttc gttggccagt tgctgagagc
     1081 ttaaatggcg cgccgtatca ggttgtagca gcagataatc cgccagtttt ttatcacttt
     1141 gggcaagccc ctggtaacgc tgacggatac gaagtaagcc gttcatatct ttcctcgcga
     1201 tgaaatagtg gcgacaacag gcaatatgag acacacgcga gtgaatttta tattccattg
     1261 ccgctaattg ccatgaatta aaaattccag ttagccatga tgcggtcgat tgttgccgga
     1321 aaagtgatga tctatctgtt aggctatgta gtagcgaaaa ttaatcagga tgtttcagtc
     1381 cagaggagta tggtttggca actcacgagc gtcgtgtggt gttttttgac ttagatggaa
     1441 cattgcatca gcaggatatg ttcggcagtt ttctgcgtta tttactgcgt cgccaaccgc
     1501 tgaatgcgtt acttgtcctg ccgttgttgc cgatcatagc cattgcgtta ttgataaaag
     1561 gtcgtgcggc acgctggccg atgagtctgc ttctgtgggg atgcactttt ggtcacaatg
     1621 aagcgcgttt gcaggcgttg caggccgatt tcgtacgctg gtttcgtgac aatgttaccg
     1681 cctttccgct ggttcaggag cgattaacca cctacctgtt aagttccgat gctgatatct
     1741 ggttgattac cggctctccg cagccgctgg ttgaagcggt ttatttcgat acgccctggc
     1801 tgccgcgggt taatcttatc gccagccaaa ttcagcgtgg ctatggtggt tgggtattga
     1861 cgatgcgttg tctgggacat gaaaaggtcg cacaactgga gcgcaaaatc ggcacgccgc
     1921 tgcggctgta cagtggctat agcgacagta atcaggacaa tccgctgctt tatttctgtc
     1981 agcatcgttg gcgagtaacc ccgcgcggtg aactccagca actggaatag agtaaagcat
     2041 agcgtccgtg tataatgcgc cgcgctttta taaccggagt tttctttttg tctgaagtcg
     2101 aatttagcca cgaatactgg atgcgtcacg cgctgacgct ggcaaagcgt gcctgggatg
     2161 agcgggaagt gccggtcggc gcggtattag tgcataacaa tcgtgttatt ggcgaaggct
     2221 ggaaccgtcc gattggtcga catgatccca ccgcacatgc agaaatcatg gccctgcggc
     2281 agggcggtct ggtgatgcaa aattatcgac tgatcgacgc cacgctgtat gtcacgcttg
     2341 aaccctgtgt aatgtgtgcc ggagcaatga tccacagtcg cattggtcgc gtggtctttg
     2401 gtgcgcgtga cgcgaaaact ggcgctgcgg gatctttaat ggatgtgctg catcatccgg
     2461 gtatgaatca ccgggtggaa attaccgaag gaatactggc ggatgagtgc gcggcgttgc
     2521 tcagtgactt ctttcgcatg cgccgccagg aaattaaagc gcagaaaaaa gcgcaatcct
     2581 cgacagatta attttgtttc tcttcactcc cttttctgga aaacagcaga gagggagagt
     2641 gggtcaaata gtttgatgac gactgggaaa gaaacgagag aaaaggaaat ttctctttgc
     2701 ccagctccgt aggcgatacc gccggataat cctgcgccag ttgcatcgcc gcttctgtag
     2761 cctgcttctc tttctcctgc agataaccca ccaggctaat ctgatactta cgaatatttt
     2821 cgacataagc gtaggcttca tgcccacgag cgtagccgta agtcagcttg ctgtaatagg
     2881 gtttctggct aagtaaaggc agacgctgtt ttacgtcagc ccaactgtca ggattacctt
     2941 tggtttttgc cgtcagggcg cgggcatcca gcatatgcgc atagcccata ttgtacgcgg
     3001 caagggcaaa ccagatccgt tcgttctccg gcacactttc cggcacttta ctcatcatat
     3061 cctgcaaata acgcacgccg ccgctgatgc tctgttcggc atcggtacga tccgtaatgc
     3121 cgaggctttg cgcggtgttt ttggttaaca tcatcatgcc gcgcacaccc gtcggtgaag
     3181 tggcctgagc atcccagtgc gattcttgat aagcaatagc ggccagcaaa cgccagtcaa
     3241 tttcttcggc gtatttctca aacaggggct ttaactgcgg cagtaccgca tcgacggcgc
     3301 gtaaaaatgt gcgcgtatcg acgtaatcaa aatcatcgcc atgccccagg tatttctctt
     3361 caatgcgtgc cagcgtaccg tcttcattca tctcattgaa gaaatcgagc agagcggcgg
     3421 aaagggtatt atcgccatct aacgggctaa accaggtcac cggttgttca tcggtgatat
     3481 cgagcgctac ggcgagttcc gggtgaacgc gctgaaacag gctgatggcg acagaatcag
     3541 caatggtgta atcgagtttt ccttcgatga catcttccat taattccgca gagccttttt
     3601 tgtcgtctac cttccagctt aattccggga attttgtttc tttcagggtc tggagatcgt
     3661 taaccaccac atgacccggt gcaacggtga gttgctccgc cgtcaggttg cccagtgtac
     3721 gtgggcgata ctgacccact ttataaacca gttgttgtga cacggaataa taggtagggc
     3781 caggctgata attttttaca cgctcactgt tatagaccag gcctgccgcc agcaggtcgg
     3841 cgttaccatt atcgaggtcg tcaaacagct ggctgatatt ctgccgcacg gtcactttca
     3901 gttttacgcc gaggtaatcg gcaaactgtt tcgccagttc gtaatccagg ccaaaaggtt
     3961 tcccgttgat ttcgttataa gtcagggggg tatgaatggt gctcacacgc aactctcccc
     4021 gcgcttgaat ggcggcgata cggttgtcgg ctttaccaaa ccagggaatg gatggccaga
     4081 gagcgaccgc gagcagcagt gccagaatgc cgatgaacag ataattaatc tttaattttt
     4141 tcaattagtt aattctctgt gtcgtgcgcg tcccagcttg aaaaaacgta ataatagtga
     4201 aaggtttact cataaatgag cggcattttg cgtaaacctg cgccagttgg caacttatta
     4261 cagccattgg cggcacgcgt tgctaattca cgatggtgat tttatttcca cgcaaacggt
     4321 ttcgtcagcg catcagattc tttataatga cgcccgtttc ccccccttgg gtacaccgaa
     4381 agcttagaag acgagagact tatgatggaa attctgcgtg gttcgcctgc attgtcggca
     4441 ttccgaatca acaaactgct ggcacgtttt caggctgcca ggctcccggt tcacactatt
     4501 tacgccgagt atgtccattt tgctgacctc aatgcgccgt taaacgatga tgagcacgca
     4561 caacttgaac gcctgctgaa atatggcccg gcactcgcca gccacgcccc gcaaggcaaa
     4621 ctcctgctgg tgaccccgcg tcctggcacc atctctccct ggtcttcgaa agcgaccgat
     4681 atcgcccata attgcggtct acaacaggta aaccgccttg agcgcggcgt tgcttactat
     4741 gtagaagccg gtacgctgac caatgaacaa tggcagcagg ttaccgctga actgcacgac
     4801 cgcatgatgg aaacggtctt ttttgcttta gatgatgcag agcagctgtt tgcacaccat
     4861 caaccgactc cggtcaccag cgttgatttg ctggggcagg gccgtcaggc gctaatcgac
     4921 gctaacctgc gtcttggcct ggcactggcg gaagatgaaa ttgactattt gcaggatgct
     4981 ttcacaaagc tgggtcgtaa cccgaacgac atcgaactgt atatgtttgc ccaggcgaac
     5041 tccgagcact gccgccacaa aatttttaac gccgactggg ttatcgatgg tgaacagcag
     5101 ccgaaatcac tgtttaagat gatcaaaaac accttcgaaa ccacgccgga tcacgttctc
     5161 tctgcttata aagataacgc cgccgtaatg gaaggttctg aagtgggccg ctactttgct
     5221 gaccacgaaa cgggccgcta cgatttccat caggaacctg cgcatatcct gatgaaagtt
     5281 gaaactcaca accacccgac ggcgatttct ccgtggccgg gcgcggcgac cggttccggc
     5341 ggtgaaatcc gcgatgaagg tgcctccggg cgcggcgcga agccgaaagc tggtctggtc
     5401 ggtttctccg tgtccaacct gcgaattcct ggcttcgaac agccgtggga agaagatttc
     5461 ggtaaacctg agcgcattgt taccgcgctg gacatcatga ccgaaggccc gctgggcggt
     5521 gcggcgttta acaacgaatt tggtcgtccg gcactgaatg gctacttccg tacttatgaa
     5581 gaaaaagtga acagccacaa tggcgaagag ctgcgcggtt atcacaaacc gatcatgctg
     5641 gcgggcggga tcggcaacat tcgtgccgat cacgtacaaa aaggcgagat caacgttggt
     5701 gcgaagctgg tcgttctcgg cggcccggca atgaacatcg gtcttggcgg cggcgcggcg
     5761 tcttccatgg cgtctggtca gtctgatgcc gacctcgact tcgcttccgt acagcgcgac
     5821 aacccggaga tggagcgtcg ctgccaggaa gtgatcgacc gttgctggca gcttggtgat
     5881 gccaacccaa tcctgtttat ccacgacgtt ggcgcaggcg gtctttctaa cgccatgccg
     5941 gaactggtga gcgacggcgg gcgcggcggt aaattcgaac tgcgcgatat cctgagcgac
     6001 gaaccgggca tgagcccgct ggaaatctgg tgtaacgaat cccaggaacg ctacgtgctg
     6061 gcagttgctg ccgatcagtt accgctgttt gacgaactgt gtaagcgtga gcgcgcgcca
     6121 tacgcggtga ttggtgaagc gaccgaagaa ctgcatcttt ctttgcacga tcgtcatttt
     6181 gataatcagc cgatcgatct gccgctggac gtactgcttg gtaaaacgcc gaagatgacc
     6241 cgcgaagtac aaacgctgaa agcgaagggt gatgcgctgg ttcgtgaagg gattaccatt
     6301 gctgacgcgg tgaaacgcgt gctgcacctg ccgaccgtgg cggagaaaac tttcctggtg
     6361 accattggtg accgcagcgt aaccggcatg gtggcgcgcg atcagatggt gggtccgtgg
     6421 caggtgccgg tcgctaactg cgcggtcact accgccagcc tcgatagcta ctacggtgaa
     6481 gcgatggcga ttggcgagcg tgcgccggtt gcgctgctgg atttcgccgc ttctgcccgt
     6541 ctggcggtcg gtgaagcgtt aaccaacatc gccgcaacac aaattggcga tatcaaacgc
     6601 attaaacttt ccgccaactg gatggcggcg gcaggccacc ctggtgaaga tgcaggcctg
     6661 tatgaagccg ttaaagccgt gggtgaagaa ctttgtccgg cgctgggcct gacgatcccg
     6721 gtgggtaaag actctatgtc gatgaaaacc cgttggcagg aaggtaacga agagcgcgaa
     6781 atgacgtcgc cgctgtcgct ggtgatttct gcatttgccc gcgtggaaga tgtacgtcac
     6841 accatcacgc cacagctttc taccgaagat aacgcactgc tgctgattga tttgggcaaa
     6901 ggcaataacg cgctgggcgc aacggcgctg gcgcaggttt atcgtcagct tggcgacaaa
     6961 ccggcagatg tacgcgatgt cgcgcaactg aaaggcttct atgacgcgat tcaggcgctg
     7021 gttgcacagc gtaagctgct ggcgtatcac gaccgctctg atggcggcct gctggtaacg
     7081 ctggcggaaa tggcctttgc tggtcattgc ggcattgacg cggatatcgc ctctctgggt
     7141 gacgatcgcc tggcggcgtt gtttaacgaa gaactgggtg cggtgattca ggttcgtgcc
     7201 gctgaccgtg aagccgttga agcggtactg gcacagcatg ggcttgcgga ttgcgtccat
     7261 tatgtagggc aggcggtttc cggtgaccgt tttgtgatta ccgccaacgg gcagactgta
     7321 ttcagcgaaa gccgcactac gttgcgtgtc tggtgggcag aaactacctg gcagatgcag
     7381 cgcctgcgtg acaacccgga gtgtgccgat caggagcacc aggcgaaatc aaacgacgcc
     7441 gatccgggcc tgaatgtgaa actgtcgttc gatatcaacg aagatgtggc agcaccgtat
     7501 attgccactg gtgcgcgtcc gaaagttgcc gtgctgcgtg agcagggggt gaactcgcat
     7561 gttgaaatgg cggcagcttt ccaccgtgca ggcttcgatg caatcgatgt gcatatgagc
     7621 gacctgctgg ctggacgcac gggcctggag gatttccacg ctctggtagc gtgcggtgga
     7681 ttctcctacg gtgacgtgct gggtgctggt gaaggctggg cgaagtctat cctgttcaat
     7741 gaccgtgtac gcgatgagtt tgcaaccttc ttccaccgtc cgcaaacgct ggcgctgggg
     7801 gtatgtaacg gttgccagat gatgtctaac ctacgcgaac tgatcccagg tagtgagttg
     7861 tggccacgtt ttgtgcgcaa tacctccgat cgctttgaag cgcgtttcag cctggttgaa
     7921 gtaacccaaa gcccgtctct gctgttgcag gggatggtgg gctcgcaaat gccgattgct
     7981 gtctctcatg gcgaagggcg cgtggaagtg cgtgatgcgg cgcatctggc ggcactggaa
     8041 agcaaagggc tggtggcact gcgctatgtc gataacttcg gcaaagtcac tgaaacctac
     8101 ccggctaacc cgaacggttc tccgaatggt attacggcag tcacgactga aagtggtcga
     8161 gtcaccatta tgatgccgca cccggaacgt gttttccgta ctgtcagcaa ctcctggcat
     8221 ccggaaaact ggggcgagga tgggccgtgg atgcgtattt tccgcaatgc acgtaagcag
     8281 ttggggtaag ccgtcaaccc attggttttg cagccggatg aggcattaag caccaactac
     8341 tattataagc tcccaacttc gggagctttt ttgtgtctgt aaatcacgac aatgggtggt
     8401 ttaccgcgtc gctttctggc gacacttaac taattgattt taatggcatc taataagttt
     8461 atcttaagtt gtctcttttt agcgacacag tggctgattt acagccaaat agcgaagaag
     8521 ccataacccc ttcaaaacag cgtacaatca gtacgttacc aaactatttt ctttattggc
     8581 acagttactg cataatagta accagtggct cattcaccga cttatgtcag ccccttcggg
     8641 acgtgctaca taaaatacga atgacgcaca acaaggtgcc tgccgtccaa cttctgatat
     8701 cagcgtagct atatcaacca tcgggcgaaa cgtcgagtta ggcaccgcct tattccataa
     8761 caaagccggg taattcccgg ctttgttgta tctgaacttc ccctcggtta gcatcaggct
     8821 attcgcgtct gacgagagta acaccttgaa acgctggccc gtttttcccc gctcattacg
     8881 acaactggta atgctggcat ttttgctgat tctgctgccc ctgttggtgc tggcatggca
     8941 agcctggcaa agcctgaatg cgcttagcga tcaggcggcg ctggttaacc gcactacgct
     9001 tattgatgcc cggcgtagtg aagcgatgac caacgcggcg ctggagatgg agcgtagcta
     9061 ccgtcagtat tgcgtgctgg acgaccctac gcttgcgaag gtttatcaaa gccagcgcaa
     9121 gcgttacagc gaaatgctcg atgcccacgc aggcgtactg ccagacgata aactttacca
     9181 ggcattgcgt caggatttga acaatctggc ccaacttcag tgtaacaaca gtggtcccga
     9241 tgctgccgcc gccgcgcgtc ttgaagcctt tgccagtgcc aataccgaaa tggtgcaggc
     9301 cacgcgcaca gtggtgttct ctcgtgggca gcaacttcag cgtgaaatcg ccgaacgtgg
     9361 gcaatatttt ggttggcaat cgctggtgct atttctggtg agtctggtga tggtgctact
     9421 tttcacgcgg atgattatcg ggccggtgaa aaatatcgag cgcatgatca accgtctggg
     9481 ggaagggcgt tctctgggca atagcgtttc gttcagtgga ccgagcgagt tacgctcggt
     9541 tgggcaacgt attctttggt taagtgagcg cctgtcatgg ctggaatccc aacgccatca
     9601 atttttaaga catttatctc atgaattaaa aacgccactg gcgagtatgc gcgagggcac
     9661 tgaattactg gctgaccagg ttgtcgggcc gcttacgcca gagcaaaaag aggtggtgag
     9721 cattcttgat agcagcagcc gcaatttgca aaaactgatc gaacaactgc ttgattacaa
     9781 ccgtaaacag gcggacagtg cggtggaact ggagaatgtt gaattagcac cgctggtgga
     9841 gacagtggtt tctgctcata gcctgcccgc acgggctaaa atgatgcata ccgacgtcga
     9901 tctcaaagca acagcttgcc tggcggagcc aatgctgctg atgagcgtac tggataatct
     9961 ttactccaat gcggtgcact acggggctga atccggtaac atttgccttc gcagcagttc
    10021 acatggtgcg cgggtttata ttgatgtcat caatacaggc acgcccattc cgcaagagga
    10081 acgcgccatg atcttcgaac ccttttttca gggaagccac cagcgaaaag gggcggtgaa
    10141 gggcagcggt ctgggattaa gcattgccag ggattgtatt cgccgtatgc aaggggaact
    10201 gtatctggtc gacgagagcg ggcaagacgt ttgtttccgc attgatttac cgtcgtcgaa
    10261 aaacacgaaa taatgctaac agcccatccc atcaggctat tttatttgtc agttcggccc
    10321 cgggcagtgc tcgaaatctt cacgtacgac atgtacgctc tggtttctcc gcgctgtcca
    10381 tgtccaaact gcctgcaaca attacgccca ctgggatagg ctttaagtct ggtgaatatg
    10441 cgacacattt ttcaacgatt attgccccga cggctgtggc tggcagggct gccatgtctg
    10501 gcattgctgg gttgcgtgca gagtcacaat aagccagcca ttgatacgcc agcagaagaa
    10561 aaaattccgg tttatcaact ggctgactat ctttctactg aatgtagcga catctgggcg
    10621 ctgcaaggta aatcaaccga aaccaatccg ctttactggc tgcgggcgat ggattgtgct
    10681 gatcgtttaa tgcctgcgca gtcgcgtcag caagcccgcc aatatgacga cggcaactgg
    10741 caaaatacct ttaaacaagg gattttgctc gccgacgcca aaattacgcc gtatgagcgc
    10801 cgccagctgg tcgcgcgcat tgatgcgcta agtaccgaga tcccggcaca ggttcgccct
    10861 ctgtatcaac tctggcgcga cggtcaggcg ctgcaactgc aactggcgga agagcgccaa
    10921 cgctacagta aactccagca gtccagcgac agcgagctgg atactttgcg ccagcaacat
    10981 cacgttctac aacagcaact ggaactcact acccgcaagc tggaaaacct gaccgatatt
    11041 gaacgccagc tctcgacccg caaaccggct ggaaatttct cgccggatac gccgcacgag
    11101 agtgaaaagc ccgcgccatc cactgatgag gtcactcctg atgagccata aacctgcgca
    11161 tttattactg gtcgatgacg atccgggatt gctgaaactg cttggcctgc gcctgaccag
    11221 cgaaggctac agcgtggtca cggcggaaag tggtgctgaa ggattacggg tactgaatcg
    11281 cgaaaaagta gatttagtta tcagcgacct gcggatggat gaaatggatg gtatgcagct
    11341 gtttgctgaa atccagaaag tgcagccggg aatgccggta attattctta ccgcacatgg
    11401 ttctattccc gatgccgttg ctgcaacaca gcagggagtg ttcagtttcc tcaccaagcc
    11461 tgtcgataaa gacgcgctat atcaggcaat tgacgatgcg ctggaacaat ccgcgccagc
    11521 caccgatgaa cgctggcgcg aggcgattgt cacccgcagc ccgctgatgc tgcgtttgct
    11581 ggaacaggcg cggctggtgg cgcaatcaga cgttagcgtt ttgattaacg gtcagagcgg
    11641 caccgggaaa gagattttcg cccaggctat ccacaacgcc agcccgcgca atagcaaacc
    11701 atttattgct attaactgtg gtgcattgcc cgagcaattg ctggagtcgg agctgtttgg
    11761 tcatgcgcgt ggcgcgttta ctggcgctgt cagcaatcgc gaaggtttat tccaggcggc
    11821 ggaaggcggt acgctatttc tcgatgagat tggcgatatg cctgcaccgt tgcaggtcaa
    11881 actgctgcgt gtgttgcagg agcgtaaagt gcgcccgctg ggcagtaacc gcgatattga
    11941 tatcgacgtg cggattattt ccgccactca ccgcgacctg ccaaaagcga tggcgcgcgg
    12001 ggaattccgt gaagatctct attaccgcct caacgttgtc agcctgaaaa ttccggctct
    12061 ggcagagcgc acagaagaca ttccgctact ggcaaatcac ctgttgcgcc aggcggcaga
    12121 gcgacataaa ccgtttgtcc gcgcgttctc caccgatgcg atgaaacgcc tgatgaccgc
    12181 tagctggccg ggtaatgtac gccagttggt caacgtgatt gaacagtgcg tggcgctgac
    12241 ctcatctccg gtgattagcg atgcattagt agagcaggcg ctggagggcg aaaacacggc
    12301 gctgccaacc tttgttgagg cacgtaatca gtttgaactc aactatttgc gtaagctgct
    12361 gcaaatcacc aaaggcaacg tcacccacgc ggcgagaatg gcggggcgca accggacaga
    12421 attttataaa ctgctttccc gacacgagct ggatgcaaac gatttcaagg aatgaattgg
    12481 cgttatgtgt tacgtttagc agatcaaaag acaggcgacc ttttcaagga atagcatgaa
    12541 aaagattgat gcgattatta aacccttcaa gctggacgat gttcgcgaag cactggctga
    12601 agtcggcatt accggcatga cggtcactga ggtgaaaggt tttggccgcc agaaaggcca
    12661 taccgagctg taccgcggcg cggagtatat ggtggatttt ctgccgaaag tgaaaattga
    12721 gattgtcgta ccggacgaca ttgtcgatac ctgtgtcgat accattattc gcacggcgca
    12781 aaccggcaaa atcggtgacg gtaaaatctt cgtctttgac gtggcacggg tcattcgtat
    12841 ccgtaccggt gaagaagacg acgcggcaat ttaaaccgcg tcaagggttg ccggatgttt
    12901 ccatccggca acatcaaatc acagcacctt atgcgggcca aagcattcgt aatgaatatt
    12961 ttcctgcttc acgcccagat ccactaactg tttcgcggca aactgcatga agccaaccgg
    13021 gccgcagaga tagaactgca ttgtcggatc gctgaacgca ccttccagtt tgctcaaatc
    13081 catcagacct tcgctatcaa actgaccttt agcgcgatcg gcttcgctcg gctgacgata
    13141 ccaggtgtgc gcggtaaagc gcggcagtga cagccccagt tccttaactt catcagcaaa
    13201 ggcgtgaaca tcgccatttt ctgccgcatg gaaccagttc acttgtgctg tgtggcctgc
    13261 ttttgccagt gtgtcgagca ttgccagcat tggcgtttga ccgacaccgg cagagattaa
    13321 cgtcactggt gtgtcatctg cgacagccat aaaaaaatca cctgctggag cgaccagttt
    13381 cacgacatcg ccaacattgg cgtgattgtg cagccagttg gatacctgcc caccctcttc
    13441 gcgtttcacc gcaatacgat agcctttgcc atccggtttg cgagtcaaag agtactgacg
    13501 aatttcctga tgcgggaaac cttccggctt cagccagacg ccgagatatt gccccggacg
    13561 gtattctgcc actgcgccac cgtcgaccgg ctccagttcg aagctggtga taagcgcgct
    13621 gcgcggtgtt ttagccacaa tgcggaaatc gcgagtacct tcccagccac cggctttgct
    13681 ggcgttttcg ttatagattt ccgcctcgcg attgataaat acattagcca gtacgccata
    13741 ggctttaccc cacgcgtcca gcacttcctg gcccgggctg aacatttcgt ccagcgtcgc
    13801 caacaggtgt tcaccgacaa tgttgtactg ttccggtttg atctggaagc tggtgtgctt
    13861 ctgcgcgatt ttttctaccg ctggcagcag cgcaggcagg ttttcaatat tactggcgta
    13921 ggcggcaata gcgttaaaca gggcttcacg ttgatcgcca ttgcgctggt tactcatgtt
    13981 aaaaatttct ttgagttctg ggttatgagt aaacatacgg tcgtagaaat gggcggttaa
    14041 ctttggtccc gtttccacca gtaaagggat ggtggctttt actgtagcga tggtttgagc
    14101 gtcaagcata tggtcttcct tttttttgca tcttaattga tgtatctcaa atgcatctta
    14161 taaaaaatag ccctgcaatg taaatggttc tttggtgttt ttcagaaaga atgtgatgaa
    14221 gtgaaaaatt tgcatcacaa acctgaaaag aaatccgttt ccggttgcaa gctctttatt
    14281 ctccaaagcc ttgcgtagcc tgaaggtaat cgtttgcgta aattcctttg tcaagacctg
    14341 ttatcacaca atgattcggt tatactgttc gccgttgtcc aacaggaccg cctataaagg
    14401 ccaaaaattt tattgttagc tgagtcagga gatgcggatg ttaaagcgtg aaatgaacat
    14461 tgccgattat gatgccgaac tgtggcaggc tatggagcag gaaaaagtac gtcaggaaga
    14521 gcacatcgaa ctgatcgcct ccgaaaacta caccagcccg cgcgtaatgc aggcgcaggg
    14581 ttctcagctg accaacaaat atgctgaagg ttatccgggc aaacgctact acggcggttg
    14641 cgagtatgtt gatatcgttg aacaactggc gatcgatcgt gcgaaagaac tgttcggtgc
    14701 tgactacgct aacgtccagc cgcactccgg ctcccaggct aactttgcgg tgtacaccgc
    14761 gctgctggaa ccaggtgata ccgttctggg tatgaacctg gcgcatggcg gtcacctgac
    14821 tcacggttct ccggttaact tctccggtaa actgtacaac atcgttcctt acggtatcga
    14881 tgctagcggt catatcgact acgccgatct ggaaaaacaa gccaaagaac acaaaccgaa
    14941 aatgattatc ggtggcttct ctgcatattc cggcgtggtg gactgggcga aaatgcgtga
    15001 aatcgctgac agcatcggtg cttacctgtt cgttgatatg gcgcacgttg cgggcctggt
    15061 tgcagctggc gtctacccga acccggttcc tcatgctcac gttgttacta ccaccactca
    15121 caaaaccctg gcgggtccgc gcggcggcct gatcctggcg aaaggtggta gcgaagagct
    15181 gtacaaaaaa ctgaactctg cggttttccc tggtggtcag ggtggtccgt tgatgcacgt
    15241 aatcgccggt aaagcggttg ctctgaaaga agcgatggag cctgagttca aaacttacca
    15301 gcagcaggtc gctaaaaacg ctaaagcgat ggtagaagtg ttcctggaac gcggctacaa
    15361 agtggtttcc ggcggcactg ataaccatct gttcctggtt gatctggttg ataaaaacct
    15421 gaccggtaaa gaagctgacg ccgctctggg ccgtgctaac atcaccgtca acaaaaacag
    15481 cgtaccgaac gatccgaaga gcccgtttgt gacctccggt attcgcgtgg gtactccggc
    15541 gattacgcgt cgcggcttca aagaagcaga agcgaaagaa ctggctggct ggatgtgtga
    15601 cgtgctggac agcatcaatg atgaagccgt tatcgagcgc atcaaaggta aagttctcga
    15661 catctgtgca cgttacccgg tttacgcata agcgaaacgg ttaatctgtt gaaaaaggct
    15721 tccttcggga agcctttcta cgttatcgcg ccatcaaatc tgtcgtaact gcgcctcaac
    15781 atacaaatag ccaatcccca gcacctgttg tgcgcggctt aattgcccaa agccaatttt
    15841 cgtcgctttc acgctcggtc cttcgtcgcg gtcgaacggg ttaaatcccg tctggcgaat
    15901 aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc aaatctgatt
    15961 aatattgagg atgttgagga agttatacag actcaaacca atggcattgg cagagcgatc
    16021 aacccagctg gtgatccacg gttctccact ctgccaggca gcgattaact gcgctgttgt
    16081 cagtttttca ggatcaagct gagtattaac cggttgtgat tttagccata cccgcgcctg
    16141 tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc cacagtcgca
    16201 gacgacgcca tccggattaa cgatggtgtg accaatctgt ccgctaccat acaaacagcc
    16261 gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa cgttgatcac
    16321 gcagaaatcc cgttcctgcg aattattttg ccatttctcc gccagcgcca gcatcacgca
    16381 gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact ttacctcaac
    16441 cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg gatcaacctg
    16501 accgtggatt gccagcgcca ggttgatggt acggtccggc cacaatttac ggtggcgatg
    16561 ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa tctgtaaata
    16621 ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa tactggtggg
    16681 cgtcacgttc aggcacaacg tccagtcacc ttccggcgca atcagccatg taccgctact
    16741 atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc tttccagttc
    16801 ctgcaaaata ttactgaccg ccggaatcga aatttgcgcc agccgggcca gcgttgattt
    16861 attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat ggtggcgaat
    16921 ctgttgatta ttaatgcagg ctctcatctt cactaaacct tgtttagtta attgcgtgat
    16981 tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt taattcagta
    17041 tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga gcgcgttgag
    17101 atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga aaatcgcgtt
    17161 tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct gagcgagcag
    17221 aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa agtgatgatc
    17281 ctgccggagc tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca gcgcgatttt
    17341 gtctatcata atgaagtcat taaaccggcg ctggtggggt tgctggggcc gtggatctcc
    17401 ggcgggattg agtttaactg gccgcaacac catcgcccga ccacctttat gcccgtcgat
    17461 ttcaccctcg aagcccatga agacggcgca caaaccgtgt gggtcggcga aacggagccg
    17521 atgcacggtt tacaggtgat gacaggtttc accctgcgcc ctgaccgggc ggcactggaa
    17581 attgccagcc gcgtctataa cggcaacgcc acgccgcgtc atttcttgtg gtgggccaac
    17641 ccggcagtga aagggggtga agggcatcag agcgtcttcc cgccggatgt aacggcggtg
    17701 tttgatcacg gcaaacgggc cgtctccgct ttccccatcg ccaccggcac ttactacaaa
    17761 gtggactact ccgccggagt ggacatttct cgctataaaa atgtgcccgt tccaacctca
    17821 tatatggctg aaaaatcaca gtacgatttt gttggcgcgt ggtgtcacga tgaagatggc
    17881 ggtttgctgc acgttgccaa ccaccatatt gcgccaggta aaaaacagtg gagctgggga
    17941 cacagtgaat ttggccaggc gtgggataag agtctgaccg acaataacgg cccgtatatc
    18001 gaactgatga ctggtatttt tgccgataac cagcccgatt ttacctggct tgatgcttac
    18061 gaagagaagc gttttgagca gtatttcctg ccttaccatt ctttgggcat ggtgcaaaac
    18121 gcctcccgcg atgcggtgat taaactccag cgtagcgagc gggggattga gtgggggctg
    18181 tatgccatct ctccgttgaa aggataccgt ctggcgatcc gcgaaatcgg caaatgcaac
    18241 gcgttgctcg atgatgccgt ggccctgaca ccagcgaccg ccatccaggg cgtgttgcac
    18301 ggtatcaatc ctgaaaggct gaccattgaa ctctccgatg ccgacggcaa tatcacactg
    18361 agttatcagg aacatcagcc gcaagagttg ccgttgccgg acgtcgccaa agcgccactg
    18421 gcagcacaag acattaccag tacagatgaa gcctggttta tcggtcagca tctggagcaa
    18481 tatcatcacg ccagccgttc accgttcgat tactacctgc gcggcgtggc gctggatccg
    18541 ctggattatc gctgtaacct ggcgctggcg atgctggaat ataaccgagc agatttcccg
    18601 caagcggtgg cgtatgccac tcaggcgctg aaacgcgcac atgcgctaaa caaaaatccg
    18661 cagtgcggac aggcgagttt gattcgcgcc agtgcttacg aacgtcaggg gcaatatcaa
    18721 caagccgaag aggatttctg gcgggcggtc tggagcggca acagtaaagc aggtggctat
    18781 tatggtctgg cacggctggc ggcgcgtaat ggtcacttcg acgctggtct ggatttttgc
    18841 caacaaagtc ttcgcgcctg cccaaccaat caggaagtgc tttgcctgca taatctgctg
    18901 ctggtgttaa gtggtcgtca ggacaacgcg cgtttgcagc gcgagaaact gctgcgtgat
    18961 tatccgctga acgccactct gtggtggctg aactggttcg atggtcgtag cgaatcagcc
    19021 ctcgcgcagt ggcgcggtct gtgtcaggga cgcgacgtta acgccctgat gaccgccggg
    19081 caactgatta actggggaat gcccaccctg gcggcagaga tgctgaacgc actggactgc
    19141 cagcgcacgc tgccgcttta cttgcaagcc agcttgctgc cgaaagctga acgcggcgaa
    19201 ctggtcacaa aagccattga tgtcttccca cagtttgtcc gtttcccgaa tacgctggaa
    19261 gaagtggcgg cgctggagag tattgaagag tgctggtttg cccgtcattt actggcctgc
    19321 ttctactaca acaagtgcag ctacaacaaa gccattactt tatggcaacg ttgcgtagag
    19381 atgtcgccgg agtttgccga cggctggcgc gggttagcga tccatgcgtg gaataagcaa
    19441 cacgattatg agctggccgc gcgttatctt gataatgctt atcagcttgc gccgcaggat
    19501 gcacgtctgc ttttcgaacg ggatttgctg gataagttaa gcggtgtcac accggagaaa
    19561 cgactggcgc gtctggaaaa taatctggaa attgcgctga aacgcgacga catgaccgca
    19621 gaactgctca atttgtggca tctcacgggt caggcagaca aagcggcgga cattctcgcc
    19681 acgcgcaaat tccacccgtg ggaaggcggg gaagggaaga tcaccagtca gtttatcctc
    19741 aaccagttat tacgcgcctg gcagcatctt gatgccagag agccgcagca ggccagcgaa
    19801 ctgcttcatg ccgcgttgca ttatccggag aatttaagcg aaggccgttt accggggcaa
    19861 actgacaacg acatctggtt ctggcaggcg atatgcgcca acgcgcaggg cgatgaaact
    19921 gaagcgacgc gttgtttacg tctggcggcg actggcgatc gcaccattaa cattcacagt
    19981 tattacaacg atcagccggt tgattatctc ttctggcaag gaatggcgct gcgactgctg
    20041 ggcgaacaac acaccgcaca gcaactgttt agtgaaatga aacagtgggc gcaagagatg
    20101 gcgaaaacca gtatcgaggc ggatttcttt gctgtttcac aacctgacct gttgtcgctg
    20161 tatggcgatt tacaacagca gcataaagaa aaatgcctga tggtggcgat gctggcgtcc
    20221 gcgggactcg gggaggttgc gcactatgaa tctgctcgcg ctgaattgat ggcgattaat
    20281 ccagcctggc cgaaagcggc attattcacc accgtgatgc cttttatttt tagctacgtt
    20341 cactaaacct tattaactgt aatatttgag cggcacgcgc tgccgctcac gctcaaataa
    20401 ttaaaataag ccgttgtaaa taaaaaccct acaggaggca ctatgcctaa aaaaatgaca
    20461 agaacccgta atttattatt aatggctacg ctattgggta gcgcactatt tgccagggct
    20521 gcggataaag aaatgactat tggcgcaata taccttgata cccagggata ttacgccgga
    20581 gtgcgtcagg gcgttcagga tgcggcaaaa gattcttcag tacaggtaca gttaattgaa
    20641 actaacgctc agggcgatat ttcgaaagaa agtacctttg ttgataccct cgtggcgcgt
    20701 aatgtcgatg ccattatttt atcggcagtg tctgaaaatg gcagtagtcg taccgttcgt
    20761 cgcgccagtg aagcgggtat tccggtgatt tgctacaaca cctgtattaa tcaaaaaggt
    20821 gtcgataaat atgtctcggc gtatctggtc ggcgatccgc tggaatttgg taaaaaactg
    20881 ggtaacgctg ccgccgatta ttttattgcc aataaaattg accagccgaa aattgccgtc
    20941 atcaattgcg aagcctttga agtttgtgtg cagcgacgta aaggatttga agaggtatta
    21001 aaatcccgcg ttcccggcgc gcaaatagtc gctaatcagg aagggactgt tttagataaa
    21061 gcgatttccg ttggtgaaaa actaattatc tccacgccgg atctcaacgc cattatgggg
    21121 gaatcgggcg gtgcgacact cggcgcggta aaagcggtac gtaatcaaaa tcaggccgga
    21181 aaaattgctg ttttcggttc ggatatgacc actgaaattg ctcaggagct ggaaaacaat
    21241 caggtgctga aagcggtggt ggatatttcc ggtaagaaaa tgggcaatgc tgttttcgcg
    21301 caaactttga aggttatcaa taaacaagcc gacggtgaaa aagtgattca ggtgccaatc
    21361 gatctctata ccaaaacgga agacggtaaa cagtggctgg caacgcacgt tgatggtctg
    21421 ccctaatcgc tgattcgcag aggtccctat gttcacagca acagaggcag tcccggtagc
    21481 aaaagtggtg gcaggaaata agcgttatcc cggcgtcgtt gcgctggata acgttaactt
    21541 cacgctcaat aaaggcgaag ttcgtgcgct gttaggcaaa aacggtgcgg gaaaatcgac
    21601 cctcattcga atgcttaccg gtagcgaacg tccggatagc ggtgatatct ggattggcga
    21661 gacgcgactg gaaggtgacg aagctacgct gactcgccgt gccgctgaac tgggggtacg
    21721 cgcggtttat caggagttaa gtctggtgga agggctgaca gtggcggaaa acctctgcct
    21781 cggtcagtgg ccccgccgca acggcatgat tgattacctg caaatggcgc aggacgccca
    21841 acgttgcttg caggcgttgg gcgttgacgt tagtcctgaa caacttgttt caacactaag
    21901 cccggcgcaa aagcagctgg tggaaattgc gcgggtgatg aagggcgagc cgcgcgtggt
    21961 cattcttgat gaacccacca gctcgcttgc cagtgcggag gttgaactgg tgatcagcgc
    22021 ggtgaaaaag atgtcggcac tgggcgtggc ggtgatttat gtcagccacc ggatggaaga
    22081 aattcgccgc attgcctcct gtgccaccgt tatgcgcgat ggtcaggtgg cgggcgatgt
    22141 gatgctcgaa aacacctcca cgcatcatat tgtgtcgcta atgctcgggc gcgatcacgt
    22201 tgatattgcg ccggtttccc ctcaggaaat tgtcgatcag gccgtgctgg aagtccgtgc
    22261 gttacgccat aagcccaagc tggaggatat cagctttacg ctccgtcgtg gcgaagtact
    22321 cggcattgct ggcctgctgg gggcagggcg cagtgaattg ttgaaagcca tagttgggct
    22381 ggagacgtat gaacagggcg aaattgttat caacggcgag aaaatcactc gccccgatta
    22441 cggcgacatg ctgaaacgcg gcattggcta tacgccagaa aaccgcaaag atgcggggat
    22501 cattccctgg ctgggcgttg acgaaaatac agtgctgacc aatcggcaaa aaatcagcgc
    22561 caacggtgtg ctgcaatggt ccaccatccg ccgcctgacc gaagaggtga tgcagcggat
    22621 gacggtcaag gccgccagta gcgaaacgcc catcggcacg ctttctggcg gcaatcagca
    22681 aaaagtggtg atcggtcgtt gggtctatgc cgccagccag attttgttgc tcgacgagcc
    22741 aacgcgtggc gtcgatatcg aagccaaaca gcagatttac cgtattgtcc gcgagctggc
    22801 tgccgaagga aaaagcgtgg tgtttatctc cagtgaagtg gaggagttgc cgctggtgtg
    22861 cgaccgcatt ctgttgttac agcacggtac gttctcgcag gagtttcacg ctccggtcaa
    22921 tgtggatgag ctgatgtccg ccattctgtc tgtgcactga tgaattttac cgaggaacct
    22981 gaagatgtct gcttcgtcat tactattgcc gcagggcaag agcgtctcgc tcaaacaatt
    23041 tgtcagtcgc catattaatg agatcggttt gctggtggtg atcgccattc tttatctggt
    23101 cttctctctg aacgcgcctg gctttatctc attgaataac cagatgaacg tgctgcgcga
    23161 tgccgccacc atcgggatcg ccgcatgggc gatgacgctg attattatct ccggtgaaat
    23221 tgatgtcagc gttggtccga tggtggcttt tgtctcggtg tgcctggcat ttttgctgca
    23281 atttgaagtt ccgctggcgg tggcgtgtct gctggtgttg ctgttaggcg cgctgatggg
    23341 aacgctcgcc ggggtgctgc gcggcgtgtt taacgtgcca agttttgttg ccacgctggg
    23401 tttatggagc gccctgcgcg gaatggggct gtttatgacc aacgccttgc cagtgccgat
    23461 tgatgaaaac gaagtgctgg actggctggg gggacaattt ctcggtgtgc cggtatccgc
    23521 gctgatcatg atggtattgt ttgcgctgtt tgtgttcatc agccgcaaaa ccgccttcgg
    23581 gcgctcggtt tttgccgttg gcggtaatgc cactgcagca cagttgtgcg gtattaacgt
    23641 tcgtcgggta cgcattctta tctttaccct ttcgggatta ttagcggcgg tgaccggcat
    23701 tttgttggcg gcgcgcctcg gttccggtaa cgcaggtgcc gcaaacggtc tggagtttga
    23761 cgtcatcgcc gcggtcgtgg ttggcggtac ggcgctttcc ggtggtcgcg gctcgttgtt
    23821 cggtacgctg cttggcgtac tggtgattac gctaatcggt aacggtctgg tgctgctcgg
    23881 gattaactcc tttttccagc aggtggtgcg cggcgtcatc atcgtggtgg cggtgctggc
    23941 gaatatcttg ctgactcagc gtagcagtaa agcgaaacgc taaaccttaa cgcccctggc
    24001 gcgggtgcgg cagggatgaa aattaatcgt attgttaatg agggatttat gaaaacgatg
    24061 ctggcagctt atttaccagg aaattcgacc gtcgatctgc gggaagttgc ggtgccgacg
    24121 ccggggatta accaggtact gatcaaaatg aaatcctccg ggatttgcgg aagcgatgtc
    24181 cactatatct atcatcaaca ccgtgccacg gcagcggcac ccgataaacc gttataccag
    24241 ggatttatca acggtcatga accgtgcggg cagattgtgg cgatggggca aggctgccgc
    24301 cattttaaag agggcgaccg tgtgctggtg tatcacattt ctggctgtgg tttttgcccg
    24361 aactgccgtc gcggctttcc tatctcttgt actggcgaag gaaaagcggc ttacggctgg
    24421 cagcgtgacg gcggtcatgc cgaatatctg ctggcggaag aaaaagatct gatcctcctg
    24481 ccggatgcac tgagttacga agatggtgcg tttatcagtt gcggcgttgg tacagcgtat
    24541 gaagggattt tgcgcggcga agtttccggc agtgataacg tactggtggt cggtctgggg
    24601 ccggtcggca tgatggcgat gatgctggcg aaaggtcgcg gtgcaaaaag gatcatcggc
    24661 gttgatatgc tgccggaacg tctggcgatg gcgaaacagt taggagtgat ggatcacggc
    24721 tatttagcga ccaccgaagg tctgccgcag ataatcgccg aactcactca cggcggcgcg
    24781 gatgttgcgc ttgattgttc cggtaatgcc gcaggtcgct tactggcact gcaatccacc
    24841 gctgactggg gacgcgtggt ttacattggt gaaaccggaa aagtcgagtt cgaggttagc
    24901 gccgacctga tgcaccatca acggcggatt atcggctcct gggtgaccag tctgttccat
    24961 atggaaaaat gcgcccacga tttaacggac tggaaactgt ggccgcgtaa cgccattacc
    25021 caccgcttct cgctggaaca ggcgggagat gcttatgcac tgatggcaag cggcaaatgc
    25081 gggaaagttg tgattaactt cccggattaa ggagttagcg gatgaccatc tataccttat
    25141 cccacgggtc gctaaagctg ggcgtttctg accagggcgg cgtaatcgaa ggcttctggc
    25201 gcgatacgac gccgctctta cgtcctggta aaaaaaacgg tgtggcaacc gatgcctctt
    25261 gttttccgct ggtgccattt gcaaaccggg tgagcggcaa ccgttttgtc tggcaggggc
    25321 gtgagtatca actgcaaccg aatgttgagt gggatgcgca ctatctgcac ggcgacggct
    25381 ggctggggca gtggcaatgt gtctcgcgca atgatgatat cttgtgtctg gtgtatgaac
    25441 atcgcagcgg tgtttatcac tatcgggtaa gccagacgtt tcatttaacg gcggatacgc
    25501 tgacggtgac actctctgtc accaatcaag gggcagagac gctgccattt ggtaccggct
    25561 ggcatcctta ttttccgttg tcgccgcaaa cgcggattca ggcacaggcg agcggttact
    25621 ggctggagcg ggagcagtgg ctggcgggtg agttttgcga gcagctaccg caagaattgg
    25681 attttaacca gctcgcgccg ttgccgcgcc agtgggtgaa caatggtttt gccggatgga
    25741 atggtcaggc ccgcatcgag cagccgcagg agggttatgc catcatcata gaaacgacgc
    25801 cacctgcacc gtgttatttc atctttgttt ccgacccggc gtttgataaa ggatatgcgt
    25861 ttgatttctt ctgtctggaa ccgatgagcc atgcgccaga tgaccatcat cgcccggaag
    25921 gtggtgacct cattgcgctt gcaccagggg aatcaacaac ttcagagatg tcgttgcggg
    25981 ttgcgttact gtaatttata aaatgccgga taaggcgttc acaccgcatc cggcactcgg
    26041 cgcacgatgc ctgatgcgac gcttgccgcg tcttatcagg cctacaattg gtgttggaac
    26101 ggtaggcagg ataaggcgtt cacgccgcat ccggcatctt tcctaacgcc gatcgagaga
    26161 aatcgccccc ggcccggtaa ttgccaacag caggaatgcg ccagcgatac tgacattctt
    26221 ccagaaatta atcatatttg gcccaaccgc atcgccggtc atatcccagt aatggtgacc
    26281 aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc gccagcggac gggtgaaaaa
    26341 gccaagcacg attaatatcg cggcgggcac ttccataact accgcaataa tcgctgccag
    26401 catcggcatc ggcgcgccca gcgaggccat atattggacc gtaccgtcaa agcccatcat
    26461 tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg atacgggcaa ttaataacaa
    26521 aacggggcgt gcagcaccaa aatcaaaata acgtagtgag ttcataacca atccctttgc
    26581 aagataatgt gatttaaacg taatacacac tcttgcacct cgccacgccc ctggcattga
    26641 gatattcgct aattatcctg gttacagtga tttaagcgcg atgtcctcat ctatcagcag
    26701 tttcgcatca aacgttttgc cgctctggat ccatttgcgg attaagcgaa tctcacgccc
    26761 ctgattcagc gttaccgcac cgataagcac gccgttttgc agattaaacc aaatcgcctt
    26821 ctgagtttcc gggttgccac gacaaatcca gtcatcgcca tgcatatcgc caataaactg
    26881 taagttatca ctgtactgat cgctccagaa ccacggcggc ggcagtcgcg gaagcggtag
    26941 ccccaacatt gcggcagcgg caatttgcgc ctggttattg gcgttttccc agctttcgca
    27001 gcggtgtagt gcaccattat caatacgggt gattgccaca tcgccaccgg caaagatcgc
    27061 gggatcgcag gtgcggcaag cttcatcaat gacaatacca ttggcagtat caaggttggc
    27121 ctcgcgagcc agttggtcgt tggcgctgat accaataccg taaatcacca catcggcccg
    27181 aagcgtttcg ccactttgca gcgtcagttc tactttttca ccatcgacca catgttcaat
    27241 ggcattattg agcagaatgc gcacaccagc ctgctgatgg cgcggtaaaa gatagcgttg
    27301 cacgggcggt ggtgcattac ggcccatgac gcttgccgcc agttcaatca ctgtcacctt
    27361 acatccacgc tgcgtggcgc tggcagccag ttccagacca atagttccgg caccgacaat
    27421 cacgactgac cgttcgggct gcagaacttc tcgcagtctg gcggcatcac cggcatggcg
    27481 cagggtaaag cagcgttctc ccagtgcatc aagcaacggc agcggtcgag ctgccgcgcc
    27541 ggttgctata aaaagctgat cccagtgcca gctttcgccg ttagctaaca ctaactcttg
    27601 tgtgtcgcgg cccaatgttt tgatggttac accggaatgc agatgaacat tgttttcctg
    27661 ccaccagtga gcgggtaaca cagactgcaa ctgtggggaa tcttccagca acatggattt
    27721 cgagagcggg gggcgttcat aaggaagatg ttgctcatcg gaaaacagat gcagctcacc
    27781 gctgaaccct tgctggcgta gcaaggccgc agccattgcc gccgcttgcc cgccaccgac
    27841 aataatgatc gttttttctt tcatcgtgct tccttaaaga tccagcccag ccgctacgtg
    27901 gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc atcacaccgc ttaatgcgcg
    27961 attattgcgc cgcgatgcca acatcacata cggacccgta aaatccgccg gttgcgggaa
    28021 aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc agagactgca ttatcgaggt
    28081 ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg gccataccac acgggccgac
    28141 gccattcacc cgcactttcg gtgccagttc ataagccagt tggcgaataa gtccggttgc
    28201 ggcatgttta ctggcggtgt acagcgggcc accgccgccg ggataccagg cggcgtttga
    28261 cagtgtgaaa atcatgctgc cttcactggc gattaacgcc ggagcgcagg cttttgcgcc
    28321 cagcaggtaa ccgagtacgt tgacattaaa cagctcgtgg aagccggttt cgagcgtctc
    28381 tgcgggagtg ttaaccagtg aggcattgtg atcccagatg cccgcattgc cgataaaaca
    28441 atccagcttg ccagaacgag tcaggatctg attaaccgcg cgttgataat cggcatagca
    28501 ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat cgctgacgca gactggcgac
    28561 ttttgccgcc gacagttcca gcgtggcaac ctgcgcgcct tcttcgataa atcgctcggc
    28621 cagcgccagg cctaatcccg atccgccgcc ggtaataaag atggactcgt tatgcagatc
    28681 gctcatggct gcgcctccgg taagtcgatg aagatgtcac caccttcaac gtgtactgga
    28741 taagtggtta gcggatcggt ggcgggcagg cataacgctt tccccgtttt caggcaaaaa
    28801 ctggcggcgt gcagcgggca ctccaccgtg gcgtcatctt ccagataccc ttctgacatc
    28861 gacgcattac catggctgca acgatcgtta atggcataaa actcgccgcc tacgttgaac
    28921 agggcgatga cgggcgaggt atcgatccgg agagcctcac cctccggcac atccgcaacg
    28981 ggacacgcat aaattcgatt catcagaaca gtacactcag gttatgggag gagatgaccg
    29041 cttgatccag gacgatatcc cgttccagca agcgccagtt cccgtcctcc agacggcgaa
    29101 ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg ctctttttgt gcccgataaa
    29161 gtagataatt caccctcacg gcaaatacgt ttgggatgtc ggtttcgctt acctggcagt
    29221 tgctgattaa gtgacgagtg cgtgacggcg gttcttctgc ccaggccatg ccggtttcca
    29281 gacgggcgat tcgccgttcc agctggtctt tggtgtcatt aaaaatccag gttgtcggcg
    29341 gttgaacgcc tttgcggcgg tcgcgcgttt gtgcgttaac cgtggtgcgc atggtgtaac
    29401 gaatctcttc gtcgagctgt gccagccagt cacgaaattt ccagtcatcc agcagactgg
    29461 cttcgtgaaa gagaaactgg ctaatgcggt gatgtaactc tagtgaaact tgcgcactca
    29521 tttcatcacc tcctgctggt aagcggcggt tttatcgagc acttcctgcc agctttcgct
    29581 actcagaaga tcggcccagc gttggtacat tccacgagcg gccgtttctg agaagatata
    29641 gttagtaatg cctggaatgc cgtcgtcgcg acgcttttcc tgaccaagcc ccatttccag
    29701 acacagtttg ctgttgcggg cgcggtggcc tttcagcaat ttctggattt cacaccagtt
    29761 ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa gcacgagtgg cgctgttttc
    29821 aaaagcggct ttaacttcat cggaggcggc tttgtcagta atacagaacg cccacacttc
    29881 gacttgatca gggccgcgcg gatgccagac gcggagcgtg gcagtgccgt tgagccatga
    29941 aagcgtgggg aaaatattgt tatgacccgc caggcgcagg gcgcgaactt cacctaaacg
    30001 ttgttctgct tcggcatagg tttcgcggta atagctggaa accgcgccat cgacccagac
    30061 attagcatcc ggtttttcag taaagaagaa accgctaccg tgaccgtcct gaccaaattg
    30121 cagcgcatct ttggcggttt cccacaccgg gcgggcggtt tgtccatcac cgagacgctt
    30181 atcgctgcca tcatctttcg cccctaatac ctgaacggca gaagcatggc tgaacagagc
    30241 atgatattgg tcactggcga actgctctgc cgggaatttc cagttgcagt tgatcaccca
    30301 cttttgtaca ccgccgacaa tttcggtgcc gccttcgcga cgatccagca tgccatccag
    30361 ataccaggca atgtcaccca ggtaatcacg caggcccggt gcgctggtat cccagttgcc
    30421 aaaaatcagc cctttataac tctccacaca aggaacttcg tttagtcccc agtgggattt
    30481 acacaaccct tgtgggtagg cgcgaggttc cagcggtaca tcgatcaact cgccgttaat
    30541 gccataagac cagccgtgat acggacaggt aaaggcgcga gtgttaccgc aatctgcata
    30601 actcacacgc atggcccggt ggcggcattg gttgagaaac gccttaatgc tgccgtcttt
    30661 ctgacgcact acgacaaccg catcttcccc catgtaggtg ttaaagaaat caccatgttt
    30721 tgggatctgg ctttcgtggg cgagaaatag ccagcaacga ccgaaaatgc gctcaagctc
    30781 cagttggtaa atatccgggt cggtataaat acgcggagtg acccgaccgt tttgggtgtc
    30841 aatcagttgg taaatgttca aatctgaggg tgtggtcata ttttttaatc ctgaaagata
    30901 agatgtattg accttctatt tttgtgccat aaggatgttg ctggttggtt gctgatatgt
    30961 gaaatagtta atttctacca gctgagacgt tttttcgata acaaggggaa ggtgatggaa
    31021 ctacggcatt tacgctattt cgtcgcagtg gcgcaggcac tgaactttac ccgtgcggcg
    31081 gaaaaactgc atacctcaca gccttcgtta agcagccaga tccgcgatct tgaaaactgt
    31141 gtcggtgttc cgttgctggt gagggataag cgcaaagtcg cgttgacggc ggcgggagaa
    31201 tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg aaaatgccaa attacgggcg
    31261 cggaaaattg ttcaggaaga cagacaatta accattggct ttgtgccatc ggcggaagtg
    31321 aatttactgc caaaagtatt accgatgttt cgtctcagac agccagacac cttaattgag
    31381 ctggtgagtt taatcaccac ggaacaggaa gaaaaaattc gccgtggtga actcgacgtc
    31441 ggcttaatgc gccatcctgt ttatagcccg gagattgatt atctggagct ttttgacgaa
    31501 ccattagtgg tggtgttacc ggttgatcac cctttagcac atgaaaaaga gatcactgcc
    31561 gcgcaactgg atggcgtgaa tttcgtcagt actgatccag cgtattccgg ttcgcttgcg
    31621 ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc caaatatcgt ccaggtggca
    31681 acgaatattc tggtgaccat gaatctggtg gggatggggc tgggcgtcac tttgataccc
    31741 ggttatatga ataattttaa taccggccag gtggtatttc gacccattgc cggaaatgtg
    31801 cctgccattg ctttactgat ggcgtggaag aaaggggaga tgaagccggc attacgcgat
    31861 ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg cataattacg cattattttt
    31921 gcaaaacgcg tcgcggatcg cagtttttta gtgcttgtga actggcgtta cagacatcgg
    31981 gaagcaggct cgaatattct tatttttcaa cttgcaccaa cattcgatta accccagact
    32041 aacgcctcct gacgggaggg actcatggtt ttgcaatcca cgcgctggtt ggcgctcggc
    32101 tatttcacat acttttttag ttacggcatt tttctacctt tctggagcgt ttggcttaag
    32161 gggattggtt taacgccaga aaccatcggc ctgttattgg gggcaggtct ggttgcccgt
    32221 tttctcggga gcttgctcat cgcgccccgc gtcagcgatc cttctcgcct gatttccgcc
    32281 ttgcgcgtgc tggcactgct gacacttctc tttgctgtcg ccttctgggc gggggcgcac
    32341 gtagcgtggc tgatgctggt gatgattggc tttaacctct ttttctcacc gctggtaccg
    32401 ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc cgcttgatta cggcaaagtg
    32461 cgattgtggg gctcggtggc gtttgtcatt ggctcgtcgc tgacgggcaa actggtcact
    32521 atgtttgatt atcgggtgat cctcgcgctg ttgacgttgg gcgtggcatc catgctgctc
    32581 ggctttctca tccgtccgac gattcagcca caaggggcaa gccgccagca ggagagcacc
    32641 ggttggtctg cgtggttggc gttggttcgc cagaactggc gctttctggc ctgcgtttgt
    32701 ttattgcagg gggcgcatgc ggcctattac ggttttagcg ccatttactg gcaggctgct
    32761 ggctactcgg cctcggcggt gggctatttg tggtcgctgg gcgtggtggc ggaagtcatt
    32821 atctttgcgc tgagtaataa acttttccgc cgttgtagtg cacgcgatat gctgttgatc
    32881 tcagcgattt gcggcgtagt gcgctggggc attatgggag caactacggc gttgccgtgg
    32941 ttgatagtgg tgcaaattct gcattgtggc accttcacgg tctgccacct ggccgccatg
    33001 cgctatattg ccgctcgcca gggtagcgaa gtcatccgtt tacaggcggt ttactctgcc
    33061 gtcgcgatgg gcggcagtat tgcgatcatg accgttttcg ccggtttcct gtatcaatat
    33121 ctgggccacg gcgtgttctg ggtgatggcg ctggtggcgc ttccggcaat gtttttgcgc
    33181 ccgaaagttg ttccctcatg ctgattcgag cattttgcgg atctgctcct gttgatgtgt
    33241 tgtcaacgtc aggtccgtat agatcagcgg tggtgaaaag agcggtaacg gcatggtata
    33301 aggagcaata atcagtgccg cgctgcgcgg agcgcctgtc tgcaaaaatg cctttaccga
    33361 catatgttta atattgagcg gcagtaacgt tagttcgcgt agctgctgct caatctgcgc
    33421 ttctcgctcg ctatcattcc cggtcagtag aataatctgt ttctcatgca ggtcgttttc
    33481 ctgcattagc caggcaccaa aaatcaccgc gaccagaccg ctttcctcat cagaaagatg
    33541 gacgccgtat tcactttcaa atccggccag cgccgcgcgg gtggtgcgca ccaggcgtgg
    33601 atacaggcgg gcgaactctt ccggcagggt gttatcaata ccgatggcaa acaaactgcg
    33661 atttaacgcc tgggcgaggt gggtataaag ctgatcgcat aacccctgtt catcataaaa
    33721 acgaacattt cccagctcac gaaaatggtt taccagacgt ttgatagact tacgcagttg
    33781 tctgtcccgc tgatgcgcat cccgcagtgg atcgggaacc cgcaacatgg aaaaaagtaa
    33841 tgccataaac agtggctcat caggcggtac aggctggagc gcccgacgct gccagtggcg
    33901 tccaatttcc tgcgctacct gaaattcaag gcaggattcc gcccagcgac gttggagcgg
    33961 attaaactga ggcgtaattc cagcgtggtg ttgcagcaaa caatattgca gatacagaca
    34021 caggaaatga atatcgcgcg tttcaaagcg tttttgcagc cgtcgggagc agagattcac
    34081 taacgcttgt agattggtat cgtcgtaaaa gttacgcgca attccgcgcc gcttcagttc
    34141 actcttcagg gcgggggtga aatggctggt aataaatgac ggacacagac ggaaaccacg
    34201 tcgtagccag tgaaataaac acaaacgttg gttaagcact gtaccttcaa cccggtagct
    34261 accgtcataa ccagtagtca gtgtgagttg atgatagcgc aggatctccc gtcctgtttc
    34321 gctgatatca agactggcaa tatcatcatc cacaccatta agctcgctga aagttgccgt
    34381 ggtggcggtc aaccccggct gaaagagcgt cagcaagatc tggcagcggc gctggggagc
    34441 cgaaaggaca gatggtggag caagcgtagg catcatctgt agatctccca taagcataat
    34501 tgataagaat agctaaagtt tgctcaggga aatggcgaag ggaatgctaa tcatcagaaa
    34561 tgttggcaag cgtcacagaa tttttctcat cttaaactgt tctgaccagt ggattagcat
    34621 gtcttaaggt ggttttgtga atgattatcg tgctagcgat catgggttag ttctgcggat
    34681 tcaacgcgct aaggataaaa tccaccatct gttcgcgata aacatcgccg tgacgatcgg
    34741 aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa ccgttttggc tcttttgcca
    34801 ggctatacaa cttttcgctg tgctgccatg ggataacgtg atcagcttta ccgtgaatca
    34861 gtaaaagcgg gatcgggctg acgctggcga tgtaattttc gccgctgtaa ctctcatcaa
    34921 gtaagtagcc actgccgggg atcatctggt tggcgatggt cgcataagag gcaaatgtgg
    34981 agtcgaggat caccgcacgt atgccttcac gatcaccctg accaataaca tccaaaatat
    35041 tcgccccgcc aatactctgc ccgaacagca ccagccgttg tgggtttaca tcactgcgat
    35101 ggcgcaccac attgatggca ctttgcgtat cgtccagcaa tccggcctgg gacggcgtgc
    35161 cttttgattt accaaacccg cgataatcaa acataaaaac gttgaaatta cgctcgggta
    35221 accaactgac cagcggccag tgggcggaca tatttccggc attgccgtga gcatgaataa
    35281 tggtggcaat ggccttttca gcggggccag ttgcagaagg gataaaccaa ccatgtaggc
    35341 gagtgccatc tttagcggtg aattctactg attcggctga ccagggatcg gggccgtaga
    35401 ttttgtcgtc agggtaatag aaaaaattga tggcgatgcg cggtattaaa taaatgcaga
    35461 aggcaacaac aaagagaata aacagaattt tgagaacgcg tttgttcact ggcagcgcca
    35521 taacctttcc ttgcgacttc tttatcatcc attaatcggg gaaagatggt gggagagtgg
    35581 cgaaagaaag taaagcggtg aggaagtatt tcggaatagc gcctgaaagg cgagggcggg
    35641 tgagtgacat cacccgccag agtcattaac gcttcagggc gtcgcttaac tcgtcacgca
    35701 tgttcgccag catggcttta acaacgcgcg ggttaccagc aacgatgtta ccggtcagca
    35761 tgtagttatg accaccggtg aagtcgctga cgatgccgcc cgcttcacga accagcagct
    35821 cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa gaaaccgtca acgcgacccg
    35881 cagcaacgta agccagatcc agcgccgcag aaccggtacg acggaagtct gcacattcgt
    35941 tgaacagttt gccgacgatg ttgatgtagg tagtggcgta ctgttttgct ttgaacggga
    36001 agccggtcgc cagaatagta ccgtcgagat cgcgagcggt gctgccgcgc agacggtagc
    36061 cgttcagctg tgcgccctga ccgcgagtgg cggtgaacag ttcgttacgc ataggatcgt
    36121 aaaccacagc aacttcagtg cggcctttga tacgaacggc gatagatacc gcgaagtgcg
    36181 gcagacgttt gataaagttg gtagtgccat ccagtggatc gataacccat tgaacatcct
    36241 gatcagtacc ttcaagttca ccgctttctt cggtgatgat ggtgtgctgt gggtaagatt
    36301 tacgaatcgt gtcgataatc accgcttcgg cagctttatc tacgttggtc acgaagtcgt
    36361 tactgccttt ctggctcgct tctacagcgt ccggggtttc atagtttttg gcaattaaat
    36421 tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat cggatgcatc ggtctctctc
    36481 actggatgtt aaagaacggg aaaacggcgc agagtatagc agcgagatcg gcatctgtct
    36541 tcgttttatg ataagatgcg tgaataatct ttagatgaag aaagacaatg ctgcaaaata
    36601 ttcgaattgt gctggtggag acgtcacaca ccggcaatat ggggtctgtt gcccgtgcca
    36661 tgaaaacaat gggattaacc aatctgtggc tggttaatcc actggtgaaa cctgactccc
    36721 aggcgattgc cctggcagca ggggccagcg atgtgattgg taatgctcac atcgtcgata
    36781 cgctcgacga agcgttagcc ggttgtagcc tggtggtggg caccagcgca cgctcccgca
    36841 cgctgccgtg gccgatgctc gacccgcgcg aatgcggtct gaaaagcgtc gctgaagcgg
    36901 caaatacccc ggtggcactg gtgtttggtc gcgagcgcgt cggcttgacc aatgaagagc
    36961 tgcagaaatg ccattatcat gtcgcgattg cggctaaccc ggaatacagt tcgctgaacc
    37021 tggcgatggc ggttcaggtt attgcctatg aagtgcgcat ggcctggctg gcaactcagg
    37081 aaaatggcga gcaggtcgag catgaagaga cgccgtatcc gctggtcgat gatctggagc
    37141 gtttttatgg tcatctggaa caaacgctgc tggcaaccgg ttttattcgt gaaaaccatc
    37201 cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg tgcgcgcccg gaaagccagg
    37261 aattgaatat cctgcgcggg attctggctt ctatagagca gcagaataaa ggtaacaagg
    37321 ccgaataata gtggttgcct gatgcgacgc ttaacgcgtc ttatcaggcc tgcagtgcac
    37381 agagcccctg ggcggataag gcgttcacgt cgcatccgac aacaggtaca aacgccacga
    37441 taaaaaaatg gcactgaagg ttaaataccc gactaaatca gtcaagtaaa tagttgacca
    37501 atttactcgg gaatgtcaga cttgaccctg ctatgcaata cccccacttt tacaataaaa
    37561 aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg agactgacat ctaaagggcg
    37621 ctatgccgtg accgcaatgc ttgacgttgc gctcaactct gaagcgggcc cggtaccgtt
    37681 ggctgatatt tccgaacgtc agggaatttc cctttcttat ctggaacaac tgttttcccg
    37741 tctgcgtaaa aatggtctgg tttccagcgt acgtggacca ggcggtggtt atctgttagg
    37801 caaagatgcc agcagcatcg ccgttggcga agtgattagc gccgttgacg aatctgtaga
    37861 tgccacccgt tgtcagggta aaggcggctg ccagggcggc gataaatgcc tgacccacgc
    37921 gctgtggcgt gatttgagcg accgtcttac cggttttctc aataacatta ctttaggcga
    37981 actggttaat aaccaggaag tgctggatgt gtctggtcgt cagcatactc acgacgcgcc
    38041 acgcacccgc acacaagacg cgatcgacgt taagttacgc gcttaataaa aagaattcag
    38101 aatcaggccg gagtgctaaa cactccgtaa acacggtcgt acatccagcc ggtagcctgg
    38161 ttccttgcat tgagtgatgt acggagttta tagagcaatg aaattaccga tttatctcga
    38221 ctactccgca accacgccgg tggacccgcg tgttgccgag aaaatgatgc agtttatgac
    38281 gatggacgga acctttggta acccggcctc ccgttctcac cgtttcggct ggcaggctga
    38341 agaagcggtt gatatcgccc gtaatcagat tgccgatctg gtcggcgctg acccgcgtga
    38401 aatcgtcttt acctctggtg caaccgaatc tgacaacctg gcgatcaaag gtgcagccaa
    38461 cttttatcag aaaaaaggca agcacatcat caccagtaaa accgaacata aagcggtact
    38521 ggatacctgc cgtcagctgg agcgcgaagg ttttgaagtc acttacctgg caccgcagcg
    38581 taatggcatt atcgacctga aagaactcga agcagcgatg cgtgacgaca ccatccttgt
    38641 ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag gatatcgcgg ctatcggcga
    38701 aatgtgccgt gctcgtggca ttatctatca cgttgatgca acccagagcg tgggcaaact
    38761 gcctatcgat ctgagccagt tgaaagttga cctgatgtct ttctccggtc acaaaatcta
    38821 tggcccgaaa ggtattggtg cgctgtatgt gcgtcgtaaa ccgcgcgtac gtatcgaagc
    38881 acaaatgcat ggcggcggtc acgagcgcgg tatgcgttcc ggcactctgc ctgttcacca
    38941 gatcgtcggt atgggcgaag cctatcgcat cgcaaaagaa gagatggcga ccgagatgga
    39001 acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc aaagatatcg aagaagttta
    39061 cctgaacggt gacctggaac acggtgcgcc gaacattctc aacgtcagct tcaactacgt
    39121 tgaaggtgag tcgctgatta tggcgctgaa agacctcgcg gtttcttcag gttccgcctg
    39181 tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg ctggggctga acgacgagct
    39241 ggcacatagc tctatccgtt tctctttagg tcgttttact actgaagaag agatcgacta
    39301 caccatcgag ttagttcgta aatccatcgg tcgtctgcgt gacctttctc cgctgtggga
    39361 aatgtacaag cagggcgtgg atctgaacag catcgaatgg gctcatcatt aatcggtatc
    39421 ggaatcagga gaatttataa tggcttacag cgaaaaagtt atcgaccatt acgagaatcc
    39481 gcgtaacgtg ggttcctttg acaacaacga cgagaacgtc ggcagcggca tggtgggtgc
    39541 accggcctgt ggcgacgtga tgaagttgca gattaaagtc aacgatgaag gtatcattga
    39601 agacgcgcgt tttaaaactt acggctgcgg ttctgctatc gcttccagct ccctggtcac
    39661 cgaatgggtg aaagggaagt ctctcgacga agcgcaggcg atcaaaaaca ccgatattgc
    39721 tgaagaactt gaactgccgc cggtgaaaat tcactgttct attctggcag aagacgcgat
    39781 caaagccgcc attgcggact ataaaagcaa acgtgaagca aaataagagt tgaggtttgg
    39841 ttatgtcgat tacactgagc gacagtgcag cagcgcgagt aaataccttt ctggctaacc
    39901 gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc cggatgttca ggtatggctt
    39961 atgtactgga atttgttgac gaaccgacgc cggaagacat cgtgtttgaa gacaaaggcg
    40021 tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga cggtacgcag ctggacttcg
    40081 taaaagaagg cctgaacgaa gggtttaaat tcaccaaccc gaacgtcaaa gatgagtgtg
    40141 gttgcggcga aagcttccac gtttgatgcg cataccgata accccaccgt ggtcgcctgc
    40201 gcgtggggtt tgttttacct gattcgccgt tatcgcggcg gatcgcagcc ctgagaatgt
    40261 tatggattac ttcaccctct ttggcttgcc tgcctgttat caactcgata cccaggcgct
    40321 gagcctgcgt tttcaggatc tacaacgtca gtatcatcct gataaattcg ccagcggaag
    40381 ccaggcggaa caactcgccg ccgtacagca atctgcaacc attaaccagg cctggcaaac
    40441 gctgcgtcat ccgttaatgc gcgcggaata tttgctttct ttgcacggtt ttgatcttgc
    40501 cagcgagcag catactgtgc gcgacaccgc gttcctgatg gaacagctgg aactgcgcga
    40561 agaactggac gagatcgaac aggcgaaaga tgaagcgcgg ctggaaagct ttatcaaacg
    40621 tgtgaaaaag atgtttgata cccgccatca gttgatggtt gaacagttag acaacgagac
    40681 gtgggacgcg gcggcggata ccgtgcgtaa gctgcgtttt ctcgataaac tgcgaagcag
    40741 tgccgaacaa ctcgaagaaa aactgctcga tttttaattt ctggaagcta aacatggcct
    40801 tattacaaat tagtgaacct ggtttgagtg ctgcgccgca tcagcgtcgt ctggcggccg
    40861 gtatcgacct tggcacaacc aactcgctgg tggcgacagt gcgcagcggt caggccgaaa
    40921 cgttagccga ccatgaaggc cgtcacctgc tgccgtctgt tgttcactat caacagcaag
    40981 ggcattcggt gggttatgat gcgcgtacta acgcagcgct cgataccgcc aacaccatta
    41041 gttctgttaa acgcctgatg gggcgctcgc tggctgatat ccagcaacgc tatccacatc
    41101 tgccttatca attccaggcc agcgaaaacg gcctgccgat gattgaaacg gcggcggggc
    41161 tgctgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc actggcggcg cgggcaactg
    41221 aagccctggc aggcgagctg gatggtgtag ttatcaccgt tccggcgtac tttgacgatg
    41281 cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg gttgcatgtt ctgcgcttac
    41341 ttaacgaacc gaccgctgcg gctatcgcct acgggctgga ttccggtcag gaaggcgtga
    41401 tcgccgttta tgacctcggc ggcgggacgt ttgatatttc cattctgcgc ttaagtcgcg
    41461 gcgtgtttga agtgctggcg accggcggtg attccgcgct cggcggcgat gatttcgacc
    41521 atctgctggc ggattacatt cgcgagcagg cgggcattcc tgatcgtagc gataaccgcg
    41581 ttcagcgtga actgctggat gccgccattg cagccaaaat cgcgctgagc gatgcggact
    41641 ccgtgactgt gaatgttgcg ggctggcagg gcgaaatcag ccgtgaacaa ttcaacgaac
    41701 tgatcgcgcc actggtaaaa cgaaccttgc tggcttgtcg tcgcgcgctg aaagacgctg
    41761 gcgtagaagc tgatgaagtg ctggaagtgg tgatggttgg cggttctact cgcgtgccgc
    41821 tagtgcgtga acgagtaggc gaatttttcg gtcgtccacc gctgacttct atcgacccgg
    41881 ataaagtcgt cgctattggc gcggcgattc aggcggatat tctggtgggt aacaagccgg
    41941 acagcgaaat gctgttgctg gatgtgatcc cgctgtcgct gggcctcgaa acgatgggcg
    42001 gcctggtgga gaaagtgatt ccgcgtaata ccactattcc ggtggcgcgc gctcaggatt
    42061 tcaccacctt taaagatggt cagacggcga tgtctatcca tgtaatgcag ggtgagcgcg
    42121 aactggtgca ggactgccgc tcgctggcgc gttttgcgct gcgtggtatt ccggcgttac
    42181 cggctggcgg cgcgcatatt cgcgtgacgt tccaggtcga tgccgacggt cttttgagcg
    42241 tgacggcgat ggagaaatcc accggcgttg aggcatctat tcaggtcaaa ccgtcctacg
    42301 gtctgaccga cagcgaaatc gcctcgatga tcaaagactc aatgagctat gccgagcagg
    42361 acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc ggcgcgtgtg ctggaaagtt
    42421 tgcacggcgc gctggctgct gatgccgcgc tgttaagcac cgcagagcgt caggtcattg
    42481 acgatgctgc cgctcacctg agtgaagtgg cgcagggcga tgatgttgac gccatcgaac
    42541 aagcgattaa aaacgtagac aaacaaaccc aggatttcgc cgctcgccgc atggaccagt
    42601 cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt ttaatatgcc aaagattgtt
    42661 attttgcctc atcaggatct ctgccctgat ggcgctgttc tggaagcgaa tagtggtgaa
    42721 accattctcg acgttgcgct gcgtaacggt atcgagattg aacacgcctg tgaaaaatcc
    42781 tgtgcttgca ccacctgcca ctgcatcgtt cgcgaaggtt ttgactcgct gccggaaagc
    42841 tcagagcagg aagacgacat gctggacaaa gcctggggac tggagccgga aagccgttta
    42901 agctgccagg cgcgcgttac cgacgaagat ttagtggtcg aaatcccgcg ttacactatc
    42961 aaccatgcgc gtgagcatta acagaggtta gtatgggact taagtggacc gatagccgcg
    43021 aaattggcga agcactgtac gatgcgtatc ccgatcttga tccgaaaacg gttcgattca
    43081 ccgatatgca tcagtggatt tgcgatctgg aagatttcga cgacgacccg caggcatcga
    43141 acgagaaaat cctcgaagcg attttgttgg tctggctgga cgaagccgag tgaatgaacg
    43201 ggctgcctgg gggtagcccg ttttgctgtt aaacgaataa ggataactaa aatgacagaa
    43261 gcgatgaaaa ttaccctttc tacccaacct gccgacgcgc gctggggaga aaaagcgact
    43321 tacagcatta ataatgatgg cattaccctg catttgaacg ggacagacga tctggggctg
    43381 atccagcgtg cggcgcgcaa gattgacggt ctgggcatca agcatgttca gttaagcggt
    43441 gaagactggg atgcggatcg ctgctgggca ttctggcaag gttacaaagc cccgaaaggc
    43501 acacgtaaag tggagtggcc ggatctggac gatgcccaac ggcaggaact ggataaccgc
    43561 ctgatgatca tcgactgggt gcgtgacacc atcaacgcac cggcggaaga actggggcca
    43621 tcgcaactgg cacagcgtgc tgttgatctg atcagcaacg tcgcgggcga tcgtgtgact
    43681 taccggatca ccaaaggcga agatctgcgt gatcaaggtt atatggggct gcacacggtc
    43741 gggcgcggtt cagaacgttc cccggtattg ctggcgctgg attacaaccc aacaggcgat
    43801 aaagaagcgc cagtgtacgc gtgcctggtt ggtaaaggca tcacctttga ttccggcggc
    43861 tacagtatca aacagaccgc atttatggac tcgatgaagt cggacatggg cggcgcggca
    43921 acggttaccg gagcgctggc gtttgccatt acgcgcggac tgaacaagcg cgtgaagctg
    43981 ttcctctgct gtgcggataa cctgatcagc ggcaatgcgt tcaagctggg cgatatcatt
    44041 acctatcgca acggtaaaaa agttgaagtg atgaacaccg atgcggaagg gcgtctggtg
    44101 ctggccgatg gcctgattga tgccagtgcg cagaaaccgg aactgatcat tgatgcggcg
    44161 accctcaccg gggcggcgaa aaccgcgctg ggtaatgatt atcacgcgct gttcagtttt
    44221 gacgatgcgc ttgccgctcg cttgctggcg agtgccgcgc aggagaatga accattctgg
    44281 cgtctgccgc tggcggagtt ccaccgcagc cagctgccgt ctaactttgc cgaactgaac
    44341 aataccggaa gcgcggcgta tccggcaggt gcaagcacgg cggcgggctt cctgtcgcac
    44401 tttgttgaga actatcagca aggctggctg catatcgact gctcggcgac ttaccgtaaa
    44461 gcgccggttg aacagtggtc tgcgggcgcg accgggctag gtgtgcgcac gattgctaat
    44521 ctgttaacgg cgtaaacgtc ttatttggcc tacaaaattg cgaaaatcca atagattgag
    44581 attcccttgt aggcctgata agcgtagcgc atcaggcact tgtgccccga acgttgaatg
    44641 aattgtggtt cttatgtccg aaacaaaaaa cgaacttgaa gacctgctgg aaaaagcagc
    44701 aaccgaaccg gcgcaccgcc cggccttttt ccgtactcta ctggaatcca ccgtctgggt
    44761 gccgggtacg gcggcgcagg gcgaggctgt ggttgaagat agcgcgcttg atttacagca
    44821 ctgggaaaaa gaagacggca ccagcgtcat tccttttttt acctcgttag aagcacttca
    44881 acaggcggtt gaagacgaac aggcatttgt cgtaatgccc gttcgcacat tgtttgagat
    44941 gacccttggc gaaacgctct tccttaatgc caaactgcca accggtaaag aatttatgcc
    45001 gcgtgaaatc agtttgctga ttggtgaaga aggaaatccg ctgagcagcc aggaagtact
    45061 ggaaggcggt gaatcgctga tattatcgga agtcgcagag ccaccagcac aaatgattga
    45121 ttcactcacc accttattta aaactattaa gccggtgaag cgtgctttta tttgttcaat
    45181 taaagagaac gaagaggcac agcctaattt acttattggc attgaagccg atggtgatat
    45241 cgaagaaatt attcaggcgg cgggaagtgt agcgaccgat acattacctg gcgatgaacc
    45301 catcgatatt tgtcaggtga aaaaagggga aaaaggaatt agccacttta ttaccgaaca
    45361 tattgcgcca ttctatgaac gtcgctgggg tggttttttg cgtgacttta aacagaaccg
    45421 gataatctaa aatatgccgc cccaaagggc ggcattcggg cttacttgct gctctgaagg
    45481 ttgttaaccg ccagtaaatt gttgccggag agactccggt tattatttac ctcgcttaac
    45541 agcgcaccag aaacaataag tacgatacaa aagcaccgta tcttcattgt gcggtccatg
    45601 cgaagaccgc atcacgacag caccactggc gatattgccg cgatagcaag cgctgagacg
    45661 cgcttgctct aacggtcgcg gaacatcctt tccttaagcc agcgcccgct tgcgagacgg
    45721 cggaaaggtc cgcagtggat aatacactaa cagaacaata ataactctct tttacttgac
    45781 aaaaagagag ttactggcga gtattgtgtc cgctgagacg cgcttgcatt actacggtaa
    45841 tacaatctgc tggaaaggcc gcgaaccaaa ccagcaacaa ataaccgcct gattttggcg
    45901 gttttttgtt ttttaaagga aagtgaattt acatgtttaa gtaatatctt ttaccggtta
    45961 gtatttttaa ttttgagcag gatttgttta tatttgtctg aattcggact aataagccag
    46021 gttattttat taccgaacac ggcggaaagg gccggtgtcg ataatacgct aaataaacaa
    46081 tgattactct ctttcgcttg acaaaaagag agttactggc gagtattgct ttgctgtgaa
    46141 gcacctgcgt tgctcatgca acacaagcca atctgctgga aaggccgcga accagaccag
    46201 caataaaaaa ccgccagatt cggcggtttt ttattgcctg ttaaaagctt atttctctgg
    46261 ctcaactggt aaatctgctc gtgcgcccca ttcactccat gcgccgtcgt acagtttcac
    46321 gtttggcaca tcaagtgtcg cgagtgccaa caaaaccacg gctgccgtta caccagagcc
    46381 gcagctgacg ataataggtt tgtcataact tacgccgcga ccaaaaaata tcgcatccag
    46441 ttcatcggtc gtttttagtt caccttcgcg caccagttcc gtccacggaa catttagtgc
    46501 accgggaata tgtccgcgac gtaaacctgg gcgaggttca tcaacttctg cgttaaaacg
    46561 tgcagccggg cgggcatcga taatttgcgc cgtattttca tggcttgcca ataacacatc
    46621 ggttactttc accacggctt caggattaaa cgcggcgtta aactctcctt ccggcagctc
    46681 tactgcgcct tcttctaata gcagatcatc acgctgccag cctgcaagtc caccacccag
    46741 aatcgacact ttctctacac caaaggtgcg cagcatccac catgctcgtg gggctgagaa
    46801 aagattacct tcgtcataga caatcaggtg cttatcctgg ttaacgccta attcacgcat
    46861 cgccacggcg aacgtttccg ggcgcggcag catgtgcgga agcggggaag tgtgatcaga
    46921 aagcgcttct atatcaaaaa acactgcgcc gggaatatgt ccattcagat actcctgagc
    46981 aacgttacga tcttcctgtc caggcgacgc catgcgggca tcgataatct gaatttccgg
    47041 gtcatcaata tgttcggcga gccagtcggc tcctacaaac catgtcgtgg acataggcat
    47101 ctccattttt gccggggtta ggctaaattg tcggatgttt agtgaaaggc gacaagcaaa
    47161 agaggctgat ttggctgtct gagccacaat tctcactaaa attcggattg tatgggcgtg
    47221 atactgaatt acataacgta aattctcacg cataataatt attcgacgtt gctaacgggc
    47281 cattgtggca gggatgaaaa atgaaaaagt tacgcgtagc cgcctgcatg ctaatgctgg
    47341 cgctggcagg gtgcgacaac aacgataacg cgccaacagc ggtgaaaaaa gatgcgcctt
    47401 ctgaagttac taaagcggcc tcttcagaaa acgtgagttc agcaaaactt tccgcgccag
    47461 agcgacaaaa actggcccaa cagagtgccg gtaaggcgct gacattgctg gatctctctg
    47521 aagttcaact tgatggcgca gccacgctgg tgctgacatt ctccatccct ctcgacccgg
    47581 atcaggaatt ctcacgcgtt atccatgtcg tcgataagaa aagcggcaaa gtggatggtg
    47641 cctgggagct gtcagataat cttaaagagt tgcgtttacg ccacctcgaa ccgaaacgtg
    47701 atttgatcgt cactattggc aaggaggtca aagcactcaa caacgcaacc ttcagtaaag
    47761 attacgaaaa aactataact acccgagata tccaacccag cgtcggtttt gccagccgtg
    47821 gctcgttgtt acctggtaaa gttattgaag ggctgccggt aatggcgctc aacgttaaca
    47881 atgtcgatgt taacttcttc cgtgttaagc cagaatctct gcctgcattc attagccaat
    47941 gggaataccg caattcgctg gcgaactggc agtctgacaa actgctgcag atggcggatc
    48001 tggtttatac cggacggttt gatctcaatt ctgcgcgtaa cacccgtgaa aaattattgc
    48061 tgccgctggg cgatatcaaa ccgcttcagc aggcaggcgt gtatctggct gtgatgaatc
    48121 aggctggacg ttacgattac agtaatcccg cgacgctgtt tacgttaagt gatatcggcg
    48181 tttcagctca ccgttatcac aatcgtctgg atatctttac ccaaagcctg gaaaacggcg
    48241 cggcccagca aggagttgaa gtctcattat taaatgagaa agggcagact ctgactcagg
    48301 caaccagtga tgcccagggg catgtgcagc tggaaaatga taaaaacgcg gcattactgt
    48361 tggcgcgtaa agacggtcag acaacgctac tcgatttaaa acttccggcg ctggacttag
    48421 cagaatttaa cattgctggc gcgccaggct atagcaaaca gtttttcatg tttggcccgc
    48481 gcgatcttta tcgccctggt gaaacagtca tcctcaatgg tttattgcgt gatgcagatg
    48541 gtaaagcatt gcccgatcaa cccatcaagt tagacgtgat taaacccgac gggcaggtac
    48601 tcaggagcgt cgttagtcag ccggagaatg gcctctacca ctttacctgg ccactcgata
    48661 gcaatgcggc aaccggtatg tggcatattc gcgctaacac gggcgataac cagtatcgga
    48721 tgtgggattt ccacgtcgaa gattttatgc cagagcgcat ggcgctgaat ctgaccggtg
    48781 agaaaacccc gctaacgccg aaagatgaag tgagattctc cgtggtgggg tactacctgt
    48841 atggtgcgcc tgctaatggt aatactttgc aagggcaact tttcctgcgc ccactgcgtg
    48901 aagctgtgtc agccttacct ggtttcgaat tcggcgatat agctgccgaa aatctttccc
    48961 gcacgctgga tgaagttcag ttgacgctgg atgataaagg gcgtggcgaa gtttctacag
    49021 aaagccagtg gaaggaaacg cattccccat tacaggttat tttccagggt agtttgttgg
    49081 aatcgggcgg tcgcccggtg acgcgccgcg ctgagcaggc tatctggcct gccgatgcat
    49141 tgccggggat ccgtccgcag ttcgcctcga aatcggttta cgattatcgc actgacagca
    49201 cggtgaaaca gcccattgtt gatgaaggca gtaacgccgc ttttgacatc gtttatagcg
    49261 atgcgcaagg cgtaaaaaaa gccgtgtcgg gcttgcaggt gcgcctgatt cgcgaacgcc
    49321 gcgattacta ctggaactgg tcagaagatg aaggctggca gtcacagttt gatcaaaaag
    49381 atctgatcga aaatgaacaa actctggatc tgaaagcgga cgaaaccggc aaggtcagtt
    49441 tcccggtaga gtggggcgct tatcgtctgg aagtcaaagc gccgaatgaa gcggtcagta
    49501 gtgtgcgttt ctgggctggc tatagctggc aggacaacag cgacgggagc ggtgccgtgc
    49561 gacccgaccg tgtcacgctg aaactggata aagccagcta tcgccctggc gataccatta
    49621 agttgcatat tgccgcgcca acggcgggta aaggttatgc gatggtcgag tccagtgaag
    49681 ggccgctgtg gtggcaagag attgatgttc cggctcaagg gctggatctg acgattccgg
    49741 tcgataaaac ctggaatcgt catgatctgt atttaagtac gctggtggta cgtcctggcg
    49801 ataaatctcg ctccgcgacg ccaaaacgcg cggttggggt gttgcatctg ccgcttggtg
    49861 atgaaaaccg tcgcctcgat ctggcgctgg aaacccctac caaaatgcgc cccaatcagc
    49921 cattaaccgt gaaaattaaa gccagcaata aaaatggtga gatgccaaaa caggtgaatg
    49981 tgctggtgtc tgccgttgat agcggtgtgc tgaatattac tgattacgtc acgccagatc
    50041 cgtggcaggc gttctttggt cagaaacgct atggcgcaga catttacgat atttacggtc
    50101 aggtcattga aggtcagggg cgtctggcag ctctgcgttt tggtggtgat ggtgatgagc
    50161 tgaaacgtgg tggtaaaccg ccggtcaatc acgtcaatat tgtcgcgcag caggcgctgc
    50221 cggtaacgct caacgaacag ggcgaaggct cggttacact gccaattggc gattttaacg
    50281 gtgaattgcg cgtcatggcg caagcctgga cggcagatga cttcggaagc aacgaaagca
    50341 aagtgattgt tgctgcaccg gtgattgctg aactgaacat gccacgcttt atggcgagtg
    50401 gcgatacctc gcgcctgacg ctggatatca ctaatcttac cgataaaccg caaaaactta
    50461 atgttgccct gaccgccagt ggtttgcttg aactggtcag cgattcaccc gcagccgttg
    50521 aattagcgcc aggggtgcgt actacgctgt ttatcccggt gcgtgcattg ccgggttatg
    50581 gcgatggaga cattcaggcc accattagcg ggttagcgtt acccggggaa accgttgccg
    50641 atcagcataa gcagtggaaa atcggcgtcc gtccggcatt cccggcacaa acggtcaatt
    50701 acggtacggc gttacagcct ggtgaaacct gggcgctccc ggctgatgga ttgcaaaact
    50761 tctcgcctgt tacgctggaa gggcaattgt tgttgagcgg caaaccaccg ctgaacatcg
    50821 cacgttatat caaagagtta aaagcgtatc cgtacggctg tctcgagcaa accaccagcg
    50881 gcctgttccc gtcactttat accaatgcag cccaactgca ggcgttgggc atcaaaggcg
    50941 acagtgatga gaaacgccgt gcatcggtcg atatcggcat ttcccgtttg ctgcaaatgc
    51001 aacgtgataa cggcggcttt acgctgtggg ataaaaacgg tgacgaagag tactggctga
    51061 cggcttacgt gatggatttc ctggtccgcg caggcgaaca gggttacagc gtaccgacag
    51121 acgccattaa ccggggtaat gagcgtctgc tgcgctattt acaagatccg ggcatgatgt
    51181 cgatcccgta cgctgataat ctcaaagcca gtaaattcgc cgtacagtct tacgctgcgc
    51241 tggtgttggc ccgtcagcaa aaagcaccac tgggtgcgct gcgtgaaatc tgggagcatc
    51301 gtgcagatgc cgcttctggt ttaccgctgc tgcaacttgg cgttgcgctg aaaaccatgg
    51361 gtgatgcaat gcgtggtgaa gaagcgattg tgctggcgct gaaaacgccg cgtaatagtg
    51421 atgagcggat atggctgggt gattacggta gtccactgcg cgacagcgcg ttgatgctct
    51481 cattgctgga agagaacaaa ctgctacccg atgagcagta ctccctgttg aacacacttt
    51541 cgcagcaggc gttcggtgaa cgctggctat cgacgcagga aagtaacgcg ttgttcctgg
    51601 ctgcccgtac tcttcaggat ttaccgggca aatggcaggc gcaaaccact ttctctgctg
    51661 agccgctgac aggcgagaaa gcgcaaacca gcaatctgaa tagcgatcaa cttgccacct
    51721 tgcaggtgac caacagtggc gatcagccgt tatggctgcg tgtggatgcc agcggttatc
    51781 cgcaatccgc acctttatcg gcgagcaatg tgctgcaaat tgagcgtcat attctgggta
    51841 ctgacggtaa gagcaaatcg ctggactcgt tacgtagcgg cgatctggtg ctggtgtggt
    51901 tgcaggttaa agccagtaac agtgtaccgg atgcgttagt cgtggatctg ttgcctgcgg
    51961 ggctggaact ggaaaaccag aatctggcga acggcagcgc cagcctggag caaagtggtg
    52021 gtgaagtgca gaacttgctg aaccagatgc agcaggcgag cattaagcac atagagttcc
    52081 gtgacgatcg ctttgtggcg gcggttgccg ttgatgaata tcaaccggta acgctggtgt
    52141 atctggcgcg ggcggtgacg ccgggaacgt atcaggtgcc gcagcctatg gtggaatcaa
    52201 tgtatgttcc tcaatggcgg gcgactggtg cggctgacga tctgttgata gtcagaccgt
    52261 aaatgcctcg cctgttaacc aaacgcggct gctggataat gctggcagcc gcgcctttta
    52321 tcatcattct ggcagcatgg gcagcagata aactctggcc tctgccattg caagaagtga
    52381 atcctgcacg cgtggtggtg gcgcaggatg gtacgccact ctggcgtttc gccgatgctg
    52441 acggtatctg gcgttatccg gtaacaatcg aagatgtttc tccacgttac cttgaagcgc
    52501 tgatcaatta tgaagatcgc tggttctgga tgcatccggg ggtgaatccg ttctcggtgg
    52561 cgcgtgcagc ctggcaagat ctcacgtcgg gtcgggtcat ttccggcggc agcacgctca
    52621 ccatgcaggt ggcgcgcctg cttgatcctc accccaaaac gtttggcggc aaaattcgcc
    52681 agctctggcg cgcgttgcaa ctggaatggc atctgtctaa acgtgaaatt ctgaccttgt
    52741 atcttaaccg tgccccgttt ggcggtacgt tgcaggggat tggtgcggca agttgggctt
    52801 acctcgggaa atcgcctgcg aatttaagct attccgaggc ggcaatgctg gcggttttgc
    52861 cgcaagcgcc cagccgtctt cgcccggatc gttggccgga gcgtgccgaa gccgcgcgta
    52921 ataaagtgct cgaacggatg gccgtgcaag gtgtgtggtc ccgtgagcgg gtaaaagagt
    52981 caagggaaga acccatctgg ctggcacccc gacaaatgcc gcaactggca ctgctgtttt
    53041 cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt tactacgctg gatgccggtc
    53101 ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg acgattgcca ccgcgcagct
    53161 cactggcgat gatcgtggtt gatcataccg atatgcgtgt tcgcggctgg gtgggatcag
    53221 ttgatctcaa cgatgattca cgctttggtc atgttgatat ggtcaatgcg atccgatcgc
    53281 caggatcggt gctcaaaccg tttgtttatg gtctggcgct ggatgaaggc ttgatccacc
    53341 cggcatcgct gttgcaagac gtccccagac gcaccggtga ttaccgacct ggcaactttg
    53401 acagcggttt tcatggcccg atcagcatga gtgaggcgct ggtgcgatcg ctgaacttac
    53461 ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt tgcggcaaag ttacgcaatg
    53521 ttggattgcc gttatatttg cccaacggtg ctgcaccgaa tctttcgctc attctcggcg
    53581 gcgctggcgc aaaactggaa gatatggcgg cagcgtatac cgcgtttgct cgccacggca
    53641 aggcaggcaa attgcgctta cagcctgacg atccgctgct tgaacggcct ttaatgtcgt
    53701 cgggggctgc gtggatcatt cgccggatta tggctgatga agcgcaaccc ttgccggatg
    53761 gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg caccagctat ggctatcgtg
    53821 atgcctgggc gattggggtt aacgctcgct atgtcattgg gatctggact ggcagaccgg
    53881 acggcacgcc cgttgttggt cagtttggct ttgccagtgc cataccgctg ttgaatcagg
    53941 ttaataatat cttactgtcg cgcagtgtga atttgccgga agacccacgt cctgactctg
    54001 taagtcgtgg cgttatctgc tggccgggcg gacagtcttt ggcggaaggt gacggtaact
    54061 gtcgccgccg cctggcaacc tggctgctgg acgggagcca gccgccaact ctattactgc
    54121 cggagcagga aggcattaat ggcattcgtt tccccatctg gctggatgaa aacggcaaac
    54181 gtgttgccgc tgattgccct caggcgcgac aagaaatgat aaatgtctgg ccgctaccgc
    54241 tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg cttgccaccg gcctcgacaa
    54301 tttgcccgcc atacggtcac gatgcacaac tcccgctgca gctaacaggt gtgcgcgatg
    54361 gcgcgattat taaacgttta ccgggcgcag cggaagcgac tttgccgttg caatcaagtg
    54421 gaggggcagg tgaacgctgg tggtttctga acggcgaacc gttaactgaa cgtgggcgca
    54481 acgtgaccct gcatttgacg gataaaggcg attatcaatt gctggtgatg gatgaggtgg
    54541 ggcaaatagc gacagtgaaa tttgtcatgc aatagtctgt tttgtccctg attgttgctt
    54601 aaactgggtt tattttaaaa aaatgttacc ttcatcaata gtcaacggct ctgttgctca
    54661 ttataatccg cgccatctcg tacgctggta cagacaacaa cagaacaatt tacagaggta
    54721 aaaatggcta ttgaacgtac tttttccatc atcaaaccga acgcggtagc aaaaaacgtg
    54781 attggtagta tctttgcgcg ctttgaagct gcagggttca aaattgtcgg caccaaaatg
    54841 ctgcacctga ccgttgaaca ggctcgtggc ttttatgctg aacacgatgg aaaaccgttc
    54901 tttgatggtc tggttgaatt catgacttct ggcccgatcg ttgtttccgt gctggaaggt
    54961 gaaaatgctg ttcagcgtca ccgcgatctg ctgggcgcga ccaatccggc aaacgcactg
    55021 gccggtactc tgcgtgctga ttacgctgac agcctgaccg aaaatggtac ccacggttct
    55081 gattccgtcg aatctgccgc tcgcgaaatc gcttatttct tcggcgaagg cgaagtgtgc
    55141 ccgcgcaccc gttaataatt tcgtaaatgc cgcgtgcaaa cgtggcatcc gtgcgccaga
    55201 atttgtacaa tgcagcgccc ccggacgagc agccgctcac cggggcgttt cttttttcaa
    55261 ccctccaggg gccataacgt gtaataacga ggccggaata gcatatgtct gaacaattag
    55321 tcacacctga aaacgtcacc actaaagatg gaaaaatcaa cctgctggat ctcaaccgtc
    55381 agcagatgcg ggagtttttt aaagatttag gtgaaaaacc cttccgcgcc gatcaggtga
    55441 tgaagtggat gtatcactat tgctgcgaca actttgatga gatgaccgac atcaacaaag
    55501 tgttgcgcgg caaactgaaa gaggtggcgg agatccgcgc accggaagtg gttgaagaac
    55561 agcgttcatc tgacggcacc attaaatggg caattgccgt tggcgatcag cgcgtcgaaa
    55621 cggtgtatat cccggaagac gaccgtgcca cgctctgcgt ctcttcgcag gtggggtgtg
    55681 cgctggagtg taaattctgt tccaccgccc agcagggctt taaccgcaac ctgcgggtat
    55741 cggaaattat cggccaggtg tggcgtgcgg cgaaaattgt cggcgcagca aaagtcaccg
    55801 gtcagcgccc aatcactaac gtcgtgatga tgggtatggg cgagccgctg ctcaacctga
    55861 acaacgtcgt tccggcgatg gaaatcatgc ttgatgattt cggttttggc ctgtcaaaac
    55921 gtcgcgtcac gctttccact tccggtgtgg ttccggcgct ggataaactg ggcgatatga
    55981 tcgacgttgc gctggctatc tccctgcacg cgccgaacga cgaaattcgt gacgaaattg
    56041 tgccgatcaa caaaaagtac aatatcgaaa cgttccttgc ggcggtgcgc cgttatctgg
    56101 agaaatccaa cgccaatcag ggccgagtca ctattgaata cgtgatgctt gaccacgtta
    56161 acgacggcac tgaacacgcg caccaactgg cggaactgct gaaagatacg ccgtgtaaga
    56221 tcaacctgat cccgtggaac ccgttcccgg gcgcgccgta tggacgcagt tcgaacagcc
    56281 gtatcgaccg tttctcaaaa gtgctgatga gctacggttt caccactatt gtgcgtaaaa
    56341 cgcgtggtga tgacattgat gctgcctgtg gtcagttggc gggcgatgtt atcgaccgta
    56401 cgaaacgtac cctgcgtaaa cgaatgcagg gtgaagccat cgacattaaa gcggtctgat
    56461 agccattacg ccacggttac tttatgtacc gtggcgtaat cttttatgaa tgaagtatta
    56521 agttgcaatt gtctggtcaa tattaacggt gcgtttttgc tgactttaag gcagtatgta
    56581 acgatgcaac agtaagttag ccttagcgaa tgtgggcttt gtcacgagca cacagacggt
    56641 cttatactgt atgataacgg ttaacttaac ggatgtttcg cggtgtgggt gggcatgatg
    56701 gttcaccggc atctcaattc tcatttaaac gtacctgcag cgaatgaata ctgaagccac
    56761 gcacgaccaa aatgaagcac ttactaccgg cgctcgcctg cgtaatgctc gcgaacaact
    56821 aggacttagt caacaggccg ttgccgagcg actttgcctg aaggtttcca cggtacgcga
    56881 cattgaagaa gataaggcac ccgccgatct tgcttcaaca ttcctgcgcg gatatatccg
    56941 ctcttatgcg cgtctggtac atattccaga agaagaactg ctgccagggc tggaaaagca
    57001 ggctccacta cgggctgcaa aagttgcgcc gatgcagagt ttttccctcg gtaaacgccg
    57061 caaaaaacgt gacggctggc tgatgacctt cacctggctg gtattgtttg tggttattgg
    57121 cctgagcggt gcctggtggt ggcaagacca caaagctcag caggaagaga tcaccactat
    57181 ggccgatcaa tcttctgcgg aactgaataa taatcaaagc cagagtgtac cgttagatac
    57241 gtcgacaacc acagatcagg ctatggcgac cacgccgaca tcacctgtgg ataccaccgc
    57301 aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc cgcaacagaa
    57361 tgcggttgtt ccaccttcgc aggcaaatgt tgataccgct gcgactccgg caccagcggc
    57421 aacaacaaca ccagatggcg ctgcgccgtt gccaaccgat caggctggcg tgaccacgcc
    57481 ggcggttgac ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc tggaagtcac
    57541 cgatgctacc ggtaaaaaac tgtttagcgg tatgcagcgt aaagacggta atttgaactt
    57601 aaccggccag gcaccgtaca aactgaaaat tggtgcgcca gccgcagtac agatccagta
    57661 tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg cgcgtctgac
    57721 cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag atttttcatg
    57781 cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg gaatgtgccg
    57841 attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgcac gacagacgtc
    57901 gaagcaacgg tcaatcaaat caaggcgctg gaacgtgttg gcgctgatat cgtccgtgtc
    57961 tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagca ggttaacgtg
    58021 ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc ggaatacggc
    58081 gtcgattgtc tgcgtattaa ccctggcaat atcggtaatg aagagcgtat tcgcatggtg
    58141 gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaatgc cggatcgttg
    58201 gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct ggaatctgcc
    58261 atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt cagcgtgaaa
    58321 gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca aatcgatcag
    58381 ccgctgcatc tggggatcac cgaagccggt ggtgcgcgta gcggggcagt aaaatccgct
    58441 attggtttag gtctgctgct gtctgaaggc atcggcgaca cgctgcgcgt atcgctggcg
    58501 gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct gcgtatccgt
    58561 tcgcgcggga tcaacttcat cgcctgcccg acatgttcgc gtcaggaatt tgatgttatc
    58621 ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat ggacgtttcg
    58681 attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact cggcgtcact
    58741 ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg tctggacaac
    58801 aacgatatga tcgatcagct ggaagcgcgc attcgtgcga aagccagtca gctggacgaa
    58861 gcgcgtcgaa ttgacgttca gcaggttgaa aaataataac gtgatgggaa gcgcctcgct
    58921 tcccgtgtat gattgaaccc gcatggctcc cgaaacattg agggaagcgt tgagggttca
    58981 tttttatatt cagaaagaga ataaacgtgg caaaaaacat tcaagccatt cgcggcatga
    59041 acgattacct gcctggcgaa acggccatct ggcagcgcat tgaaggcaca ctgaaaaacg
    59101 tgctcggcag ctacggttac agtgaaatcc gcttgccgat tgtagagcag accccgctat
    59161 tcaaacgtgc gattggtgaa gtcaccgacg tggttgaaaa agagatgtac acctttgagg
    59221 atcgcaatgg cgacagcctg actctgcgcc ctgaagggac ggcgggctgt gtacgcgccg
    59281 gcatcgagca tggtcttctg tacaatcagg aacagcgtct gtggtatatc gggcctatgt
    59341 tccgtcacga gcgtccgcag aaagggcgtt atcgtcagtt ccatcagttg ggctgcgaag
    59401 ttttcggtct gcaaggtccg gatatcgacg ccgaactgat tatgctcact gcccgctggt
    59461 ggcgcacgct gggtatttcc gagcacgtaa ctcttgagct gaactctatc ggttcgctgg
    59521 aagctcgtgc gaattaccgc gatgcgctgg tggcattcct tgagcagcat aaagaaaagc
    59581 tggacgaaga ctgcaaacgc cgcatgtaca ctaacccgtt gcgcgtactc gattcaaaaa
    59641 atccggaagt gcaggtgctt ctcaacgacg ctccggcatt aggcgattat ctggacgagg
    59701 aatctcgtga gcactttgcc ggtctgtgca aactgcttga gagcgcgggt atcgcttaca
    59761 ccgtaaacca gcgtctggtg cgcggtctgg attactataa ccgtaccgtt ttcgagtggg
    59821 tgactaacag cctcggttcc cagggcaccg tgtgtgcagg tggtcgttat gacggtctgg
    59881 tggaacaact gggtggtcgt gcaacaccgg cagtcggttt tgcgatgggc ctcgaacgtc
    59941 ttgtattgtt agttcaggcc gttaatccgg aatttaaagc cgatcctgtt gtcgatatat
    60001 acctggtggc ttcaggtgct gacacacaat ctgcggctat ggcattagca gagcgtctgc
    60061 gtgatgaatt accgggcgtg aaactgatga ccaaccacgg cggcggcaac tttaagaaac
    60121 agtttgcccg tgctgataaa tggggtgccc gcgttgctgt ggtgctgggt gagtctgaag
    60181 tggctaacgg cacagcagta gtgaaggatt tgcgctctgg tgagcaaact gcagttgcgc
    60241 aggatagcgt agccgcgcat ttgcgcacgt tactgggtta aggaaggaga aggacagcgt
    60301 ggaaatttac gagaacgaaa acgaccaggt tgaagcggtt aaacgctttt ttgctgaaaa
    60361 tggcaaagca ctggctgttg gggtgatttt gggcgttggc gcactgattg gctggcgcta
    60421 ctggaatagc catcaggttg attctgcacg ctccgcttct cttgcctatc aaaatgcggt
    60481 taccgcagtg agcgaaggca aaccggatag catcccagcg gcggaaaaat ttgctgctga
    60541 aaataaaaat acttatggtg cgctggcttc tttggaactt gcgcagcaat ttgttgacaa
    60601 aaatgaacta gagaaagctg ccgcccagtt acaacagggg ctggcagaca cgagcgatga
    60661 aaatctcaaa gccgtgataa atctgcgtct tgctcgcgtt caggtacagc tcaagcaggc
    60721 tgatgccgcg ctaaaaaccc ttgatgctat caaaggtgaa gggtgggctg ccattgttgc
    60781 cgacctgcgt ggtgaagcat tgctgagcaa aggtgataag caaggtgcgc gtagtgcatg
    60841 ggaagcaggc gtgaaaagcg atgttacccc ggcactgagc gaaatgatgc agatgaaaat
    60901 taataatttg tccatctgag agggacccga tgcaattgcg taaattactg ctgccaggac
    60961 tgctttccgt taccctttta agcggctgtt cgctgtttaa cagcgaagaa gatgtggtaa
    61021 agatgtcccc attgccaacc gttgaaaacc agtttacgcc gactactgcg tggagcactt
    61081 ccgttggtag cggcattggc aacttctatt ccaatcttca tccggcactg gcggacaacg
    61141 ttgtctatgc agcggaccgc gctggtttag taaaagcgct gaatgcggat gacggcaaag
    61201 aaatctggtc tgtcaacctg gccgagaaag atggctggtt ctctaaagat cctgcattac
    61261 tttctggcgg cgtgaccgtg tctggtgggc atgtctacat tggcagcgaa aaggcgcagg
    61321 tttacgcgct gaataccagc gatggtactg tggcatggca aactaaagtc gcgggtgaag
    61381 cactttcgcg cccggtggtc agcgacggtc tggtgttaat ccacaccagt aacggtcagt
    61441 tacaagcgct gaacgaagct gacggcgctg tcaaatggac agttaacctc gatatgccgt
    61501 cgctctcttt gcgtggcgag tctgcgccgg caacggcttt tggtgcggcc gtcgtgggtg
    61561 gcgataatgg tcgcgtcagc gcagtgctga tggaacaggg ccagatgatt tggcagcagc
    61621 gtatttccca ggcgaccggt tctaccgaaa ttgaccgtct gagcgatgtt gacacgactc
    61681 ccgtcgttgt taacggcgtt gttttcgcgc tggcctataa tggtaacctg acggcgcttg
    61741 atctgcgcag cggtcagatt atgtggaaac gcgaactggg ttcggtgaat gatttcatcg
    61801 tcgacggcaa ccgcatctat ctggtcgatc aaaatgaccg ggtgatggcg ttgaccattg
    61861 atggcggcgt tacgctgtgg acacaaagcg atctgctgca tcgcctgctg acttctccgg
    61921 tgctgtataa tggcaacctg gtggtaggtg acagtgaagg ttatctgcac tggattaacg
    61981 tcgaagatgg tcgtttcgtt gcccagcaaa aagttgatag ttccggtttc cagactgaac
    62041 cggttgctgc tgacggcaaa ctgctgatcc aggcaaaaga cggaaccgtg tactctatta
    62101 cacgttaatc gtctctgtcg ttcactttga aaacggctcc tggacagggg ccgttttcct
    62161 gtttttaaca acgacacgaa tatagcgtgc gttgtctgat gatttataaa aatgaggctt
    62221 taaacatggt acctgtggtc gcgcttgtcg ggcgccctaa cgtaggaaaa tccacgttat
    62281 ttaaccgtct aactcgcacc cgagatgcgc tggttgcgga tttcccgggt ctgactcgtg
    62341 accgtaagta cggtcgtgcg gaaattgaag gccgcgagtt tatctgcatt gataccggcg
    62401 ggattgatgg caccgaagac ggtgtagaga cacgtatggc ggaacagtcg ctgctggcga
    62461 ttgaagaagc ggacgttgta ctgtttatgg tggatgcgcg cgcgggcctg atgccggcgg
    62521 atgaagcgat tgctaaacat ctgcgctccc gtgaaaaacc gaccttcctg gtggcaaaca
    62581 aaactgacgg tctggatccc gatcaggcag tggttgattt ctacgcgctt ggtttaggtg
    62641 aaatctaccc gatcgccgcg tctcacggtc gtggcgtatt aagtctgctg gagcacgtgc
    62701 tgctgccgtg gatggaagat ctcgcaccgc aagaagaagt cgacgaagac gccgaatact
    62761 gggcgcaatt tgaagcggaa gagaacggcg aagaagaaga ggcagacgac ttcgacccgc
    62821 aaagtctgcc gatcaaactg gcgattgtgg gtcgtccgaa cgtaggtaag tctacactca
    62881 ctaaccgtat tcttggtgaa gagcgcgttg ttgtttacga catgcctggc acgacgcgtg
    62941 acagtatcta cattccaatg gaacgcgacg gacgtgagta tgtgctcatt gacaccgctg
    63001 gtgtacgtaa acgcggcaaa atcaccgatg cggtagagaa attctcggta atcaaaacgt
    63061 tgcaggccat tgaagatgcc aacgtggtga tgttggtgat tgatgcgcgt gaaggtattt
    63121 ccgatcagga tctctccttg ctgggcttta ttctcaatag tgggcgctca cttgtgattg
    63181 tagtgaacaa gtgggacggc ctgagccagg aagtgaaaga gcaggtgaaa gagacgctgg
    63241 acttccgtct gggctttatc gattttgctc gtgtgcactt tatctctgcc ttgcacggca
    63301 gtggtgttgg taacttgttt gaatcagtac gtgaagcgta tgacagctcc acccgtcgtg
    63361 tggggacctc tatgctgacg cgcatcatga cgatggctgt tgaagatcac caaccaccgc
    63421 tggtacgcgg tcgtcgtgtg aagctgaaat acgcccacgc cgggggttat aacccgccga
    63481 ttgtggtgat tcacggcaat caggtgaaag acctgcctga ttcctacaag cgttacctga
    63541 tgaactactt ccgcaaatcg ctggacgtaa tgggatcgcc gattcgtatt cagttcaaag
    63601 aaggggaaaa cccgtatgcg aacaagcgta acaccctgac gccaacccag atgcgtaaac
    63661 gtaagcgtct gatgaagcat atcaagaaaa gtaagtaatc acttatctgg tttccctttc
    63721 cacacggaga gggaagccga ataacacgcg aggagtattt atggcactca actgtccaac
    63781 ctgcaaaacc cctctcgaac gcaatggcga gactgctcac tgtgcaactt gcgacaaaga
    63841 ctttaccgtc caggcacttt gccctgagtg tcatcaggcg ttacaagtgt taaaagcttg
    63901 tggtgctgtg gattatttct gccaacatgg gcacggttta atatctaaaa aacgcgttga
    63961 atttattatt gcttaatatc atgtgaatgt attgttttat catttatatt taat
//
LOCUS       DAZGTJ010000016        63931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000016 DAZGTJ010000000
VERSION     DAZGTJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.016"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(13..834)
                     /gene="dapB"
                     /locus_tag="ACHK8E_001338"
     CDS             complement(13..834)
                     /gene="dapB"
                     /locus_tag="ACHK8E_001338"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP0343629.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1001..1915)
                     /gene="rihC"
                     /locus_tag="ACHK8E_001339"
     CDS             complement(1001..1915)
                     /gene="rihC"
                     /locus_tag="ACHK8E_001339"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP0343630.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(1981..2931)
                     /gene="ispH"
                     /locus_tag="ACHK8E_001340"
                     /gene_synonym="lytB"
     CDS             complement(1981..2931)
                     /gene="ispH"
                     /locus_tag="ACHK8E_001340"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP0343631.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(2933..3382)
                     /gene="fkpB"
                     /locus_tag="ACHK8E_001341"
     CDS             complement(2933..3382)
                     /gene="fkpB"
                     /locus_tag="ACHK8E_001341"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0343632.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3490..3984)
                     /gene="lspA"
                     /locus_tag="ACHK8E_001342"
     CDS             complement(3490..3984)
                     /gene="lspA"
                     /locus_tag="ACHK8E_001342"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP0343633.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(3984..6800)
                     /gene="ileS"
                     /locus_tag="ACHK8E_001343"
     CDS             complement(3984..6800)
                     /gene="ileS"
                     /locus_tag="ACHK8E_001343"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP0343634.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(6843..7784)
                     /gene="ribF"
                     /locus_tag="ACHK8E_001344"
     CDS             complement(6843..7784)
                     /gene="ribF"
                     /locus_tag="ACHK8E_001344"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP0343635.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(7792..8010)
                     /gene="yaaY"
                     /locus_tag="ACHK8E_001345"
     CDS             complement(7792..8010)
                     /gene="yaaY"
                     /locus_tag="ACHK8E_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP0343636.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8113..8376
                     /gene="rpsT"
                     /locus_tag="ACHK8E_001346"
     CDS             8113..8376
                     /gene="rpsT"
                     /locus_tag="ACHK8E_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP0343637.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8472..9377)
                     /gene="nhaR"
                     /locus_tag="ACHK8E_001347"
     CDS             complement(8472..9377)
                     /gene="nhaR"
                     /locus_tag="ACHK8E_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP0343638.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9437..10603)
                     /gene="nhaA"
                     /locus_tag="ACHK8E_001348"
     CDS             complement(9437..10603)
                     /gene="nhaA"
                     /locus_tag="ACHK8E_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP0343639.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            11189..11341
                     /gene="mokC"
                     /locus_tag="ACHK8E_001349"
     CDS             11189..11341
                     /gene="mokC"
                     /locus_tag="ACHK8E_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP0343640.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(11445..12575)
                     /gene="dnaJ"
                     /locus_tag="ACHK8E_001350"
     CDS             complement(11445..12575)
                     /gene="dnaJ"
                     /locus_tag="ACHK8E_001350"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP0343641.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(12664..14580)
                     /gene="dnaK"
                     /locus_tag="ACHK8E_001351"
     CDS             complement(12664..14580)
                     /gene="dnaK"
                     /locus_tag="ACHK8E_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP0343642.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            14956..15360
                     /gene="yaaI"
                     /locus_tag="ACHK8E_001352"
     CDS             14956..15360
                     /gene="yaaI"
                     /locus_tag="ACHK8E_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP0343643.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            15386..16099
                     /gene="msyB"
                     /locus_tag="ACHK8E_001353"
     CDS             15386..16099
                     /gene="msyB"
                     /locus_tag="ACHK8E_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0343644.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            16248..16814
                     /gene="satP"
                     /locus_tag="ACHK8E_001354"
     CDS             16248..16814
                     /gene="satP"
                     /locus_tag="ACHK8E_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP0343645.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(16849..17436)
                     /gene="mog"
                     /locus_tag="ACHK8E_001355"
     CDS             complement(16849..17436)
                     /gene="mog"
                     /locus_tag="ACHK8E_001355"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP0343646.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(17551..18504)
                     /gene="tal"
                     /locus_tag="ACHK8E_001356"
     CDS             complement(17551..18504)
                     /gene="tal"
                     /locus_tag="ACHK8E_001356"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0343647.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            18783..20213
                     /gene="yaaJ"
                     /locus_tag="ACHK8E_001357"
     CDS             18783..20213
                     /gene="yaaJ"
                     /locus_tag="ACHK8E_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP0343648.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            20283..21059
                     /gene="yaaA"
                     /locus_tag="ACHK8E_001358"
     CDS             20283..21059
                     /gene="yaaA"
                     /locus_tag="ACHK8E_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP0343649.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(21099..21395)
                     /locus_tag="ACHK8E_001359"
     CDS             complement(21099..21395)
                     /locus_tag="ACHK8E_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP0343650.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(21609..22895)
                     /gene="thrC"
                     /locus_tag="ACHK8E_001360"
     CDS             complement(21609..22895)
                     /gene="thrC"
                     /locus_tag="ACHK8E_001360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP0343651.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(22896..23828)
                     /gene="thrB"
                     /locus_tag="ACHK8E_001361"
     CDS             complement(22896..23828)
                     /gene="thrB"
                     /locus_tag="ACHK8E_001361"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP0343652.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(23830..26292)
                     /gene="thrA"
                     /locus_tag="ACHK8E_001362"
     CDS             complement(23830..26292)
                     /gene="thrA"
                     /locus_tag="ACHK8E_001362"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP0343653.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(26318..26437)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(26373..26438)
                     /gene="thrL"
                     /locus_tag="ACHK8E_001363"
     CDS             complement(26373..26438)
                     /gene="thrL"
                     /locus_tag="ACHK8E_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP0343654.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(26652..27338)
                     /gene="yjtD"
                     /locus_tag="ACHK8E_001364"
     CDS             complement(26652..27338)
                     /gene="yjtD"
                     /locus_tag="ACHK8E_001364"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0343655.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(27738..27878)
                     /gene="yjjY"
                     /locus_tag="ACHK8E_001365"
     CDS             complement(27738..27878)
                     /gene="yjjY"
                     /locus_tag="ACHK8E_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP0343656.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            27974..28690
                     /gene="arcA"
                     /locus_tag="ACHK8E_001366"
     CDS             27974..28690
                     /gene="arcA"
                     /locus_tag="ACHK8E_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP0343657.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(28749..30101)
                     /gene="creD"
                     /locus_tag="ACHK8E_001367"
     CDS             complement(28749..30101)
                     /gene="creD"
                     /locus_tag="ACHK8E_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP0343658.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(30159..31583)
                     /gene="creC"
                     /locus_tag="ACHK8E_001368"
     CDS             complement(30159..31583)
                     /gene="creC"
                     /locus_tag="ACHK8E_001368"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP0343659.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(31583..32272)
                     /gene="creB"
                     /locus_tag="ACHK8E_001369"
     CDS             complement(31583..32272)
                     /gene="creB"
                     /locus_tag="ACHK8E_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP0343660.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(32285..32758)
                     /gene="creA"
                     /locus_tag="ACHK8E_001370"
     CDS             complement(32285..32758)
                     /gene="creA"
                     /locus_tag="ACHK8E_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP0343661.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            32969..33838
                     /gene="robA"
                     /locus_tag="ACHK8E_001371"
     CDS             32969..33838
                     /gene="robA"
                     /locus_tag="ACHK8E_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP0343662.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(33835..34482)
                     /gene="gpmB"
                     /locus_tag="ACHK8E_001372"
     CDS             complement(33835..34482)
                     /gene="gpmB"
                     /locus_tag="ACHK8E_001372"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP0343663.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            34534..35049
                     /gene="yjjX"
                     /locus_tag="ACHK8E_001373"
     CDS             34534..35049
                     /gene="yjjX"
                     /locus_tag="ACHK8E_001373"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP0343664.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(35043..35369)
                     /gene="trpR"
                     /locus_tag="ACHK8E_001374"
     CDS             complement(35043..35369)
                     /gene="trpR"
                     /locus_tag="ACHK8E_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP0343665.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(35459..37396)
                     /gene="sltY"
                     /locus_tag="ACHK8E_001375"
     CDS             complement(35459..37396)
                     /gene="sltY"
                     /locus_tag="ACHK8E_001375"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP0343666.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            37732..39399
                     /gene="ettA"
                     /locus_tag="ACHK8E_001376"
     CDS             37732..39399
                     /gene="ettA"
                     /locus_tag="ACHK8E_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP0343667.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(39455..39739)
                     /gene="nadS"
                     /locus_tag="ACHK8E_001377"
     CDS             complement(39455..39739)
                     /gene="nadS"
                     /locus_tag="ACHK8E_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0343668.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(39741..40073)
                     /locus_tag="ACHK8E_001378"
     CDS             complement(39741..40073)
                     /locus_tag="ACHK8E_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0343669.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(40165..41397)
                     /gene="nadR"
                     /locus_tag="ACHK8E_001379"
     CDS             complement(40165..41397)
                     /gene="nadR"
                     /locus_tag="ACHK8E_001379"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP0343670.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(41418..42800)
                     /gene="radA"
                     /locus_tag="ACHK8E_001380"
     CDS             complement(41418..42800)
                     /gene="radA"
                     /locus_tag="ACHK8E_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP0343671.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(42849..43817)
                     /gene="serB"
                     /locus_tag="ACHK8E_001381"
     CDS             complement(42849..43817)
                     /gene="serB"
                     /locus_tag="ACHK8E_001381"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP0343672.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            43923..44567
                     /gene="ytjB"
                     /locus_tag="ACHK8E_001382"
     CDS             43923..44567
                     /gene="ytjB"
                     /locus_tag="ACHK8E_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP0343673.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            44595..45611
                     /gene="lplA"
                     /locus_tag="ACHK8E_001383"
     CDS             44595..45611
                     /gene="lplA"
                     /locus_tag="ACHK8E_001383"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP0343674.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            45643..45906
                     /locus_tag="ACHK8E_001384"
     CDS             45643..45906
                     /locus_tag="ACHK8E_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0343675.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(46067..46786)
                     /gene="deoD"
                     /locus_tag="ACHK8E_001385"
     CDS             complement(46067..46786)
                     /gene="deoD"
                     /locus_tag="ACHK8E_001385"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP0343676.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(46843..48066)
                     /gene="deoB"
                     /locus_tag="ACHK8E_001386"
     CDS             complement(46843..48066)
                     /gene="deoB"
                     /locus_tag="ACHK8E_001386"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0343677.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(48118..49440)
                     /gene="deoA"
                     /locus_tag="ACHK8E_001387"
     CDS             complement(48118..49440)
                     /gene="deoA"
                     /locus_tag="ACHK8E_001387"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP0343678.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(49518..50297)
                     /gene="deoC"
                     /locus_tag="ACHK8E_001388"
     CDS             complement(49518..50297)
                     /gene="deoC"
                     /locus_tag="ACHK8E_001388"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP0343679.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            50555..52105
                     /gene="yjjI"
                     /locus_tag="ACHK8E_001389"
     CDS             50555..52105
                     /gene="yjjI"
                     /locus_tag="ACHK8E_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP0343680.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            52077..52940
                     /gene="yjjW"
                     /locus_tag="ACHK8E_001390"
     CDS             52077..52940
                     /gene="yjjW"
                     /locus_tag="ACHK8E_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP0343681.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(53158..53937)
                     /gene="yjjV"
                     /locus_tag="ACHK8E_001391"
     CDS             complement(53158..53937)
                     /gene="yjjV"
                     /locus_tag="ACHK8E_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0343682.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(53934..55007)
                     /gene="yjjU"
                     /locus_tag="ACHK8E_001392"
     CDS             complement(53934..55007)
                     /gene="yjjU"
                     /locus_tag="ACHK8E_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP0343683.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(55129..55290)
                     /gene="ytjA"
                     /locus_tag="ACHK8E_001393"
     CDS             complement(55129..55290)
                     /gene="ytjA"
                     /locus_tag="ACHK8E_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP0343684.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(55417..56022)
                     /gene="osmY"
                     /locus_tag="ACHK8E_001394"
     CDS             complement(55417..56022)
                     /gene="osmY"
                     /locus_tag="ACHK8E_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP0343685.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(56415..58004)
                     /gene="prfC"
                     /locus_tag="ACHK8E_001395"
     CDS             complement(56415..58004)
                     /gene="prfC"
                     /locus_tag="ACHK8E_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP0343686.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(58095..58772)
                     /gene="yjjG"
                     /locus_tag="ACHK8E_001396"
     CDS             complement(58095..58772)
                     /gene="yjjG"
                     /locus_tag="ACHK8E_001396"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP0343687.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(58787..59233)
                     /gene="rimI"
                     /locus_tag="ACHK8E_001397"
     CDS             complement(58787..59233)
                     /gene="rimI"
                     /locus_tag="ACHK8E_001397"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0343688.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(59202..59615)
                     /gene="holD"
                     /locus_tag="ACHK8E_001398"
     CDS             complement(59202..59615)
                     /gene="holD"
                     /locus_tag="ACHK8E_001398"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP0343689.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            59718..60749
                     /gene="rsmC"
                     /locus_tag="ACHK8E_001399"
     CDS             59718..60749
                     /gene="rsmC"
                     /locus_tag="ACHK8E_001399"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP0343690.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            60949..61035
                     /locus_tag="ACHK8E_001400"
     tRNA            60949..61035
                     /locus_tag="ACHK8E_001400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60983..60985,aa:Leu,seq:cag)
     gene            complement(61074..61310)
                     /gene="yjjZ"
                     /locus_tag="ACHK8E_001401"
     CDS             complement(61074..61310)
                     /gene="yjjZ"
                     /locus_tag="ACHK8E_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP0343691.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            61451..62239
                     /gene="fhuF"
                     /locus_tag="ACHK8E_001402"
     CDS             61451..62239
                     /gene="fhuF"
                     /locus_tag="ACHK8E_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP0343692.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(62276..62953)
                     /gene="bglJ"
                     /locus_tag="ACHK8E_001403"
     CDS             complement(62276..62953)
                     /gene="bglJ"
                     /locus_tag="ACHK8E_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP0343693.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(62911..63636)
                     /gene="yjjQ"
                     /locus_tag="ACHK8E_001404"
     CDS             complement(62911..63636)
                     /gene="yjjQ"
                     /locus_tag="ACHK8E_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0343694.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        15304 a        17334 c        15879 g        15414 t
ORIGIN      
        1 aatattttgt ggttacagat tattgagatc aagcacatct cgcatatcaa aaagaccact
       61 ttccttacca ctcaaccaca aagccgatct taccgcaccg ttagcaaatg tcatgcggct
      121 ggacgcctta tgggtgatct ccagacgctc gccaatatcg gcaaacatcg cggtatgttc
      181 accaacgatg tcacctgcac gcacggtggc aaaaccaatg gtgccaggca cacgttcacc
      241 ggtatggcct tcacgactgt agaccgcgca atctttcaga tctttatcca gagcgtgggc
      301 gatcgcctct cccattgcca gtgcggtgcc tgacggcgca tcaactttat gtctatgatg
      361 tgcttcaata atttcgatat cggtgtagtc acccatcact ttggctgctt tctccagcag
      421 tttaagcatg acgttaacgc caacgctaaa attggcggca aagacaatcg caatatcggc
      481 ggcggcgtca cgaatcgcct gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat
      541 ccctttgcca tgctggcgac aaaaagcgag atggttcagc gtaccttccg gacgggtaaa
      601 atcgataaac acatcaaaat catcttttat cgcatcaaga ctactttgca cagtaacgcc
      661 tgttttcccg gctccggcca gttcgcccgc atcgctgccc agtaaagaag atccttcgcg
      721 ctccagcgcc gcgcccagct gcacgccttc taatgccagc gccgcctgaa tcaactggcg
      781 gcccatacgg cccccggctc ccgcgatggc aacgcggatg tttgcatcat gcatagctat
      841 tctcttttgt taatttgcat agaccgtttt cagagtaacc agcgcatcgc tacgccgcca
      901 gccaaaaacg ctgataatta gaaattaatg ataaacaggg agggatatca gtgaaaggca
      961 tgagtcgatg aatgactgca tgccgataac atgtgacagg ttacggcacc agagccagca
     1021 cctcagctac ccactgctgg aagcctttta cattcagatc caatgccacc tgtacattgg
     1081 ctggtttgcc caggcaaccg tcgatatcaa ccaccgtcgt gccagaggta aattcgccct
     1141 gcgtttccac tgccacaaaa cacggcttga gagtgaacag gtccgggcgc accagccagg
     1201 cgatggcgca gagatcgtgc attcgcagtc cactttgcat actgccgcta cggtagtgac
     1261 taaacagggc gtggagcatt tttccggtgc gattcaactc cggcaaggta gcgagatagt
     1321 caggggttaa tattgcctga ttggtgacat ccagaccgca catgacgatt tcgatgccgc
     1381 tacggaagac acaggctgcc gcttctggat cggcagcgat attaaactcg gcgtttggtg
     1441 tgcagttgcc gcgaccagca gaaccgccca tgatcaccag acggcgaata tacggcttac
     1501 attccgggca ttgtgaaagt aacagcgcaa tattggttaa cgggccgatg gccaccaggg
     1561 taacgggctc tggcgcacgc atcagggcat cacgaattgc cagaaacgcc ggtataccca
     1621 gtggctgacg gttgtgttca acaaagtcat agccagccat tcccgattca ccgtgtacag
     1681 atgccgcatc gcgcggcgca cgaaccagtg gtacggcagc gccctgggca agcggaatct
     1741 ccgcattcca gaaatgcagc agttgcaggg cgttgcgggt cgttttctct accgaaacat
     1801 tacccgcgac agtggtcatc agttgcagat cgagttcggg ggtaaaaatc gcggcggcaa
     1861 tggcaacggc atcgtcaatg ccggggtctg tatcgaggaa gataggtaaa cgcatgtttt
     1921 ctccataaaa aatgccggta acaagaccgg cattttcgca taacttaggc tgctaatgac
     1981 ttaatcgact tcacgaatat cgacacgcag ctctttcggc acttcgaaaa caatgttttc
     2041 ttcacgacct tccagcggaa tggcttcacc accgcccagc tgttgcaaac gtgccaccac
     2101 attctgcacc agaatatccg gagccgatgc gcccgcagtc acgccaacgc agttcacgcc
     2161 ttttacccac tcttcctgga tatctttcgc atcgtcaatc aaaaatgcgc gtttgcccat
     2221 acgctgggcc agttccgcca gacggttgga gttggaggag tttttcgaac caaccaccag
     2281 tactacttcc gcctgctctg ccagggcgcg taccgcctcc tgacggttag tcgtggcgta
     2341 gcagatgtca tctttgcgcg gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat
     2401 cacatcagac gtgtcatcca ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc
     2461 gtttttgacc gtcagtttcc acacatcgtc tggcgattcg accagataca ttcccccttc
     2521 cgggttgctg tactggccca tcgtcccttc cacttccggg tgcccggcgt gaccgatgag
     2581 aatagattct tcaccacgac ggctggcgcg ggcgacttcc atatgcactt tggtcaccag
     2641 cggacaggtg gcatcaaaca ccgtcaaatc gcggcttttc gcttcgttac gtactgcctg
     2701 agaaacaccg tgtgcggaga aaatcaggat cgcgccgtcc ggtacttcgc taatctgctc
     2761 aataaagata gccccacgct cgcgcaagct atcgaccacg tagcggttat gcaccacttc
     2821 gtgacggaca tatatcggtg cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc
     2881 gcggtctacc ccggcacaaa aaccacgcgg gttggccaac aggatctgca tgttacgcct
     2941 ccagtgccgg atcgatttcc agcacttcaa tatcaaaatg aacggtctgc ccggccagcg
     3001 gatggttgaa atcaacggta atggagtcgc cgttaatttc gcggatcacg ccaggcatct
     3061 cactgccatc cattgcggta aaaagcatga ttgcgccaat ttctggctcg cctgcatcca
     3121 taaattcacg gcgggagaag tactgaatca ggtccggtga cggcacgcca aacgccgcat
     3181 caggctccag cgagaaggtg gttttatcgc ccactttcag ccccagcagg tgttgctcca
     3241 gcccttcaga aagagaagca tcacccaggc ggaacagcgc cggtttaccg ttgttgcggg
     3301 tagactcagc ggtggtgcca tcatcgagtt tcagcgtgaa gtgcaccagg acggcgctat
     3361 tgctctgtac agattcagac atgcaggttt gctcttatat tttgaggatt tttgccggat
     3421 gcggcctacg atttcgcagc aatctgtagg ccggataaga tgcgtcagca tcgcatccgg
     3481 cagggtttat tattgttttt tcgctttaga aggtaaaaaa ccttccagca caatcagtgc
     3541 cgcaccgaca caaatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac
     3601 atagaagtcg atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag
     3661 cgcgccgcca ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg
     3721 atacatcatc actgccagga tcacgctaat accaatcgca ataccggcaa aaaaccaacg
     3781 ctgccagccg ccactatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg
     3841 cagattaagc gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag
     3901 gtatttgctg cccagatcga taatcagcac gactaccacc agccacagcc agcgtagccc
     3961 tgttgaacag atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg
     4021 ttgctgacac aacggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg
     4081 tagtgccagc agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagccct
     4141 ttgaggactt cgctctgctg ggcgtcagca ggtgcgtcgt tatagtctgc aacggtagcg
     4201 ccggaggtca acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt
     4261 tccggttctg catacaaggt tactgccgct tccagcgagc cgcccacttt cttatcggca
     4321 cgcgcttgct caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac
     4381 gcatcgttca ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg
     4441 aagacgtatt tttcacgttc gcccggcagg taaccccaca cttcatcagc ggtgaaggag
     4501 aggattggtg ccatccagcg aaccagcgct tctgcgatgt gatacagcgc agtctggcag
     4561 ctacgacgcg ccacgctgtc cgctttggcg gtatactgac ggtctttgat gatgtcgagg
     4621 tagaaggacc ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa
     4681 tcgtatgctt cgtacgcttt gaggatgtct tcctgtgccg cgttcgcaca acctacggcc
     4741 cagcgatcca gtaccaccat ctcttccggt ttcaccatat cttttgccgg atcaaaaccg
     4801 ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac gacgatagct gtcggcagca
     4861 cgtttcagga tctcgtcaga aacggccatt tcgccagtgt agtcggttga tgccacccac
     4921 agacgcagaa tatccgcacc cagtttgttc atcacatcct gcggcgaaac ggtgttaccg
     4981 atggatttag acatcttgcg accctgacca tccacggtaa agccgtgagt cagtacctga
     5041 cgatatggcg ctttgccctt catcgcggta gagatcatca gggaagacat gaaccagcca
     5101 cggtgttggt cagaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc
     5161 acatcaacaa cggaagagtg ggtagatccg gagtcaaacc atacatccag cgtatccggt
     5221 actttcacat actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac
     5281 gcctgaatgc cgtcaacttc aacgcgtttt gccacttctt ccatcagttc gagagtacgc
     5341 ggatgcagtt cttctgtgtc tttgtgcacg aacagtgaca tcggcacgcc ccaggtacgc
     5401 tgacgagaga tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc
     5461 cagtcaggga tccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc
     5521 tgatccatgc tgacgaacca ctgcggcgtc gcgcggaaga tgatcggcgt tttatgacgc
     5581 cagcagcacg gatagctgtg ttgcattttc tcaacgtgca acagtgcgcc tttttcctgc
     5641 aacaacgcaa tgacaatatc gttcgctttg aagacgttaa cgccatccag agtcgggtaa
     5701 gtacccggca gataagtgcc gtccgggcca accgggttag cggtttccag accatatttt
     5761 tgaccgatca catagtcgtc cggaccgtgg cctggcgcgg tatgaacggc accggtaccg
     5821 gcatccagcg taacgtggtc gccgagaatt gccggaacat cgaagtccat aaacggatgg
     5881 gtaaagcgca acagttccag ctcggcacct ttcaccgtgc caagaatggt gtaatcgcta
     5941 acgccgatac gctgcattac gctttcaacc agatctttcg cgaggatcac ggcctgaccg
     6001 tcgatttgca ccagcgcata atcaaaatca ggtgcgatgg agattgcgcg gttagcaggc
     6061 agcgtccacg gcgtggtggt ccatataacc agcgaaattg ggccgttaac attgcttacg
     6121 ccaaatttcg ttttaagcgc atcctgatcg accgcctgga aagcgacgtc gatggacaga
     6181 gaagttttgt cgtaatactc aacttccgct tctgccagtg cagaacggca gtcaacgcac
     6241 cagtgcaccg gcttcgcgcc tttgtgcagg tgaccgttgc cgatgatttt gcccagcgcg
     6301 cggatgatgt tggcttcagt tttgaagtcc atggtcaggt acgggtgcga ccagtcgccc
     6361 agcacgccca gacggataaa gtctttgcgc tgaccgtcaa cctgggtcgc agcgtattcg
     6421 cggcacttgg cgcggaactc agcggcggtg aatttctccc ccggcttacc gtattcttgc
     6481 tctactttca gctcgattgg cagaccatga cagtcccagc caggcacata cggcgagtca
     6541 tatccagaaa gccctttgga cttaatgata atgtctttca gaatcttgtt aaccgagtga
     6601 ccaatatgaa tactgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg
     6661 ccttttttag ctgcacgaat gatgccgtac agatcatcat cagtccaacg cgccagcatt
     6721 cccggttcgc gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg
     6781 gttgatttat agtcactcat cagattctcg gttccgtatt tcggtttgat tacataacag
     6841 gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc
     6901 tgcgctttca gttcgtccag cgatgcaaat cgctgctcat tgcgtatttt tttacgcagc
     6961 actacttgta tatggcgacc ataaaggtcc attgcaacat ctaacaaatg cacttccagt
     7021 tgctggcgaa taccggcaac cgttgggcgt gttccgatgt ttgcaacgcc gggtaacggc
     7081 ttttcgccaa ggcccaacac ttctaccgca taaacccctt tcaccgggga aacctgacgg
     7141 cgtagcggta cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact
     7201 acacgcccgg agatagcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca
     7261 tccgcaagcg cctgacgcac ggcggtgctg ctgatacgca cgccaccttc gcaaaaagtt
     7321 tgcgtgctgg tgatatcgaa gccgtattcc atgcccgctt tctgtaataa caagaaatcg
     7381 ccttcacgac cagcgccaaa gcggaaatcg tcacctacgg caagaaattt tacccgcaag
     7441 tgcttcacca gaagatcact gatgaagttt tgcgcggtta acgccgcaaa acgcctgtca
     7501 aaacgcacgc acagcacgta atcaacacca cactctgcaa gataacgcag tttttcccgc
     7561 agccgggtga gccgtgccgg ggctttatca gtagcaaaca gttccagtgg ttgaggttca
     7621 aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac
     7681 agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct
     7741 tgcggggcct ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag
     7801 tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga
     7861 aatcagaaag aagtggcttt tcattgctat ggcgcaaatc atgggaagaa actgaccgcc
     7921 tgctgcaatt tttctcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc
     7981 gccatcacta cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga
     8041 caaaagaagg ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt
     8101 gggagttgga ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag
     8161 gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac
     8221 gcagctatcg aagctggcga caaagctgct gcacagaaag catttaacga aatgcaacca
     8281 atcgtggacc gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag
     8341 gctaacctga ctgcacagat caacaaactg gcttaattgc ctgttgttgt cagctttgta
     8401 aaaaaacccg ctctggcggg tttttttata tccatctgca ggccaaaatt cggcctgcaa
     8461 agtacaaatg tttattttga agctggagta aacagcgcag aatagtctgt attgcagata
     8521 cgctgtactg cagggtgctg aatcatcctt tcggcaaaaa tcgcgtggta ctcttccatc
     8581 acgttctcaa cacggcctat ctccacaacc gagtcatcgt tatagaaatc attagcgtaa
     8641 agcgaaggtg caacgaaaat agcgttatgc gtcgccccaa aggctttcat caacgcagca
     8701 tcatcaaact cacccaaaat ttcgacgttc aagccctggg agttaaacca gtttaatagc
     8761 ttacgcccca acattgagcg acgccccgga ataagtaaac gacgctcttc aagacaggca
     8821 ggaaacggct tttctggtag tgggttagtg caccagaaac tgacaccaca ttcgccaatt
     8881 ttcatggaaa acagcccttc ctgctgagtg gaatcgatcg gacagtcaga gatgatcata
     8941 tccagtttat gctgactcaa ctgctctaaa agcatctcgt gcgtcgattc gaaacagcgt
     9001 agatggatct gctcgtcttc cacaactgcg gcatccagaa cactgctgac cagacgtttg
     9061 gaaagtgcat ctgccacacc aacatcaaac aataagttgg actctttgcg atagttgacg
     9121 atatccagca tttcctggct taaggtgaac attttatcgg catagcgata gaccagctct
     9181 cccagctcgc tgggctcgag gccacgtccc ttgcgcttaa ataatttgcc ttgcaggcgc
     9241 tcttccagcg cccgaatctg tccggtaatg gtttgtggtg ttaaataaag cgcctccgct
     9301 gcgccaacca cggaaccttc tttatagaca tgccagaagt aatacaagtg gttgtaattg
     9361 atatgagaca tgctcatttc tctccctgat aacaatgaaa agggagccgt ttatggctcc
     9421 ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac gcgtaaccag ctgtatccaa
     9481 ttaccgccga agagatagaa ccgaccagga taccgagttt cgcccagtta atcagttctg
     9541 gatctacgct accaaaggcc aggctggcaa taaagataga catagtaaaa ccgataccgc
     9601 acaggatccc caccgccata atttgctgat aagtcgttcc ctcaggcaga tgcgccagtt
     9661 tcaaacgcag cgccaaccag cagaacagac taatccccag cggtttgcca atcagcaagc
     9721 cagcgatgat ccccaatggc agaatggagg tcaagccatc cagcgtgacg ccttgcagtg
     9781 aaacgccagc attagcaaat gcaaacagcg gtaaaatcag atacgccacc cacgggtgca
     9841 acacatgctc cagtcgcttc gctggagaac gcccatgctt ctctttcaaa ggaataaaga
     9901 agccgacaat tacccccgcc agggttgcgt gaacccccga tttcaacacc gcagtccaca
     9961 acaccacgcc aaccagaata tagacgcccg tgcggcgtac accacacaga ttcaataccg
    10021 cgagtaccgc aattgctaca gccgcgacgc caagagaggc catcgataag tcattagtgt
    10081 agaacaatgc gatgataatg atggccccaa gatcgtcgat aatagccaga gccatcaaaa
    10141 agatcttcag ggctaacgga acacgacttc ccaacagcgc cagcacacca agtgcaaaag
    10201 caatgtcagt agccgccggg atcgcccacc cttcgcgggt aatcggatcg gcatggttaa
    10261 aggccagata gagtaatgcc ggcacaatca tcccaccaat agcggcgata actggaaatg
    10321 cagcctggcg taagctggct agcgatcctt gcatcagttc acgtttaact tccagaccga
    10381 ctaacaggaa aaataccgcc atcagcgcgt catttatcca taacagcatg tttttgttga
    10441 tttcgagtga accaacccgg agctgaaccg gcgtctccag aaagtcgtga taccatccac
    10501 tggttgcgcc gctgttggcc ataatcatcg ccaggatagc ggcaatgata agaataatgc
    10561 ctcccgaggc atcactgcta aagaatcgat gcagatgttt cactttttat ttctctttca
    10621 ggtgaataga tcgtttttac tatcttaccc cgcacgaatc atcgttaaaa tcagattata
    10681 gtgtaggaat aattcgcttt ttacgaggca ttgaaggatt tgcgtcatca gtgatgagat
    10741 gagtttttga cggctgtaag cgctgacgtg tgaccctaag ccctgataat catagcttaa
    10801 ggtcaggatt tctgagggat taattaggaa aagtaataat taatttgttg tcagtcggcg
    10861 atgtttgtta ggccaatact tcaaaaataa tccagtttgt cgtcaataat tgcgatgaag
    10921 agataaatta aagttaaata attaaatcat taaaaaatta cgccataaaa atgcgaggcg
    10981 tcttcaggat tagaatcgat aaaacagaat gggttaaaga gagcgatata atagcggcgg
    11041 gtgcttgagg ctgtctgtct caggcattag ctgaacggca gatagagaaa agccccgagt
    11101 gatattttac tatcaacccg aggcccccta tatgcagaac acatgtagag tgcctcttac
    11161 tgaccgtaag gtcaaggaga agagagcaat gaagcagcct aaggcgatga ttgtcgccct
    11221 gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag acctctgtga
    11281 ggttcacatc cgaactggcc agacggaggt tgctgttttc acggcttacg aatccgagta
    11341 agagcaacgg cggggagtga tccccgccac ttttcaggtg ctcgcatatc ttcaacgcac
    11401 cctattttta cccaggcctg cccacgggca ggcttttggg gagattagcg ggtcaggtcg
    11461 tcaaaaaact tcttcacacc atcaaagaag ctctttgagc gcgggctgtt gtgctcgccg
    11521 gttgggccac cgaagctttc ttgcagctct tgcagcagct gtttctgctt ctcgttcagg
    11581 cctaccggtg tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc accgcggaca
    11641 gacttgacgc ctttaccgcg catacggaac agcttaccgg tctgggtttc gccaggcact
    11701 ttcagtttga cgcgaccatc aagggtcggt acttcgattt cgccacccag cgccgccata
    11761 gcgaagttga tcgggacttc gcaatagagg ttgttgcctt cacgctcgaa aatcgggtgc
    11821 tgtttaacct gaacctgaac gtacagatcg cctgccggtg cgccatgctc gcccgcttca
    11881 ccttcgcccg caagacggat gcggtctcca gtgtccaccc ctgccgggat tttaacggac
    11941 agcgttttgc tgcgctcaac acgaccatga ccatgacatt tgttgcacgg atctttgatc
    12001 agcgtaccgc ggccctgaca gtgtggacag gtctgctgca cggcaaagaa accctggcgc
    12061 atctgcacct gaccagaacc atgacaggtc ggacaggtct gcggctgtgt acctggtttt
    12121 gcaccgctac cgtggcaaac gtcacactct tccagagtcg gaatgcggat ctctttggtc
    12181 acgccacgta cagcttcttc gagggtgagc tccatgttat agcgtaaatc agcaccgcgc
    12241 gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc accaaaaata
    12301 tcgctgaagt ctgcgccgcc gccgaaaccg ccgcctccca tgccaccttg ctcaaacgca
    12361 gcatgaccat actgatcgta tgccgcacgt ttttgcgagt cggtcagaac ttcataagct
    12421 tccttgatct ctttaaattt cgcctcggcc tctttgtcac cctggttacg gtccgggtgg
    12481 tatttcatgg ccaggcgttt gtaggccttt ttgatttcac gctcttccgc tgttttggaa
    12541 acgcctaaaa tctcgtaata atcttgctta gccatctttt ttaaattgcc cctagatgaa
    12601 tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt ataattaccc gtttataggg
    12661 cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg acaacatcgt catctttcgc
    12721 gttgtttgca gaagcatcag caccggcagt ctgctgctgg gcatgttgct gctgggcgat
    12781 ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt ttcgcttcga tagcggcttt
    12841 gtcttcacct ttcagagcag tttccagtgc agtcagtgca gactcgatag cagttttgtc
    12901 gtcagccggc agtttgtcgc ccacttcttc aacctgctta cgggtgctgt gcagcagatg
    12961 gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta cggtcagctt cggcgttagc
    13021 ttctgcgtcg cgtaccattt tctggatttc atcttcgttc agaccagaag aagccttgat
    13081 ggtgatcttc tgctctttac cgctgttttt atctttcgcg gaaacgtgca ggataccgtc
    13141 agcatcgata tcgaaggtaa cttcgatctg cggcatgccg cgcggtgccg ggttgatacc
    13201 atccaggttg aactgaccca gagatttgtt atcagctgca cgtttacgtt caccctgcag
    13261 cacatggatg gttaccgcag actggttgtc ttcagcggta gagaacacct ggctgtgctt
    13321 ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca tggtttcgat
    13381 acccagagac agcggggtaa cgtccagcag cagtacgtct tttacgtcac cagtcagaac
    13441 accaccctga acagcagcac cgattgctac agcttcgtcc gggttaacgt ctttacgcgg
    13501 ctctttacca aagaactcag caactttctt ctgaaccatt ggcatacgag tctgaccacc
    13561 aacgaggata acgtcgtcga tatcagatac ggacaggcca gcgtcctgca gtgcaacttt
    13621 cagcggctca atggaacggt ttaccagatc ttcaaccagg ctttccagtt tcgcacgagt
    13681 cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca gtgatgtacg gcaggttaac
    13741 gtcggtctgc tgagcggaag acagttcgat tttcgctttt tctgccgctt ctttcaggcg
    13801 ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga tctttcttga attcttcaac
    13861 caggtagttg atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat caccgttggt
    13921 tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg atttcgataa tagaaatatc
    13981 gaaagtacca ccacccaggt cataaaccgc gatagtacgg ttgccagtac ctttgtccag
    14041 accgtaagcc agcgcagctg cggtcggttc gttgatgata cgttttactt ccagaccagc
    14101 gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg ccggtacggt
    14161 gataacagct tcagttaccg gttcacccag gtaatcttca gcggttttct tcattttttt
    14221 cagcacttca gcagagatct gcggcggtgc cattttctgg cctttaactt cgacccatgc
    14281 gtcgccgtta tcagcagcaa taattttgaa cggcatgatg gaaacatcac gctgtacttc
    14341 ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca aacagggtgt tttgcgggtt
    14401 cgtcactgcc tgacgtttag ccggctgacc aaccagagtt tcaccatcct gggtataggc
    14461 aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc agtacacgag gagtggtgcc
    14521 atccataatc gctacacaag agttggtagt acccaggtcg ataccaatta ttttacccat
    14581 ctaaacgtct ccactatata ttcggtcatc atgtggttgt gagtctgtaa taggggcgaa
    14641 acgtctggtt tcaactgccc aatttcattt ttttgcagac tcactcactg cggttgacta
    14701 ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt tgtgcttttg
    14761 cgcagaaatt tcgcgatatg ccagaacaag acatgaaact gacagcaatt ttattcatat
    14821 agagaatgaa tagcagacaa gacattcata gaaaaattgt aataaaaaga aaatattacg
    14881 tacctgaatc ttctctttat cagcagtaaa ctagtgggta ttcatcccct acctctcccc
    14941 actaagagaa tccttatgaa atccgttttt acgctttccg ccagcctggc gattagcctg
    15001 ctgctgtgct gcacggcgca ggcaaacgac cataaaatcc tcggcgtcat tgcaatgcca
    15061 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gccgaattgt taaacgcata
    15121 caactctcag ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
    15181 gcccgtagcg ccagtcagac cctgaatatt ccttcagaaa taaaagaagg gcaaaccact
    15241 gactggatca acattaacag cgataacgac aataaacgct gcgtctcaaa aatcaccttt
    15301 tctggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
    15361 cgccagaata tagaagccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
    15421 ttttctccag cattgtagtg aggaacaact ggcaaatttc gcccggctgc tcacccataa
    15481 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
    15541 ggaaggacat cccgagcaac accgccgcaa ctggcagctg attgccggag aattacaaca
    15601 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
    15661 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
    15721 aattgagcaa cagttactgg aacaatttct gcgtaatacc tggaagaaaa tggacgagga
    15781 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
    15841 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
    15901 attgacccgc attttacgca cccacgcagc aatgagcgta cttgggcatg gtttgcttcg
    15961 cggcgcgggg ctgggaggcc ctgtaggcgc ggcactaaat ggggttaaag cggtcagcgg
    16021 cagtgcctat cgcgtgacaa ttccagccgt actgcaaatc gcctgcctgc gccggatggt
    16081 tagtgccact caggtctgat accggaaacc ggggaaaatt ttcattttcc cctcaaaaga
    16141 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tacagttatt
    16201 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
    16261 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
    16321 acctgcacaa cgtgggttat tttgctctgg acggtattat tcttgccatg ggcattttct
    16381 acggcggcat cgcgcaaatt tttgctggtc tgctggagta caaaaaaggc aacactttcg
    16441 gtttaaccgc attcacctct tacggttctt tctggctgac gctggttgcg attctgctga
    16501 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
    16561 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
    16621 aattcgtttt ctttagcctg accgtgctgt ttgccctgct ggcgatcggt aacattgccg
    16681 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgcggt gccagcgcaa
    16741 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
    16801 gtgaatccca ttaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
    16861 gtcgcgtctt gcactcttcg gtctgaatgc tgcaaccact tccggtgccg tttcaatgta
    16921 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccat gcaccacaac
    16981 gttacccgca gcgtccttca cgccttccag cgtctcttta atcgacttcg gctgaccggg
    17041 taagttaagg atcagcgcct gtttgcgaat cacgcccacc tgacgcgaaa ggatcgcagt
    17101 tggtacaaaa tgcaggctga tctggcgcat ctgttcacca aagccaggca tctcgcggtc
    17161 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
    17221 ggtgagcacc agatggcaac tcatttcatc caccagctca cacaacgttt gctcgatgat
    17281 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
    17341 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
    17401 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
    17461 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga aaaggtagcg
    17521 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
    17581 tttttcctgg tcaacagcaa acttacggat accttccgcc agtttatcta ctgccattgg
    17641 atcctggtta tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
    17701 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
    17761 cagcgccggt gcgatggtca gacggtcgca gcctgccagt tccagaattt cgccgatgtt
    17821 acggaagctt gcgcccatca ccacggtttc ataaccgtgc tctttgtagt actggtagat
    17881 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tgtcggtatt
    17941 cgctttgtac cagtcaagaa tacggccaac aaacggcgag atcaggaaca cgcccgcttc
    18001 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt taataccttc
    18061 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
    18121 acgatcgttg ctaatccctg catcgttgta gagtttgatc aggcgttttg ctttcgcaat
    18181 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
    18241 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
    18301 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
    18361 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
    18421 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
    18481 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
    18541 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
    18601 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
    18661 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
    18721 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
    18781 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
    18841 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
    18901 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
    18961 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggctggcg
    19021 ttgcgctggc tattaccacc ggtggaccag gtgccgtctt ctggatgtgg gttgccgcgt
    19081 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
    19141 acgttaatgg gcagtttcgt ggcggaccgg catggtatat ggcgcgcggg ctggggatgc
    19201 gctggatggg cgttctgttc gccctctttt tgctcatcgc ctacggcata attttcagcg
    19261 gagttcaggc gaacgccgtt gcccgagccc tgagtttttc ttttgatttt cccccgctgg
    19321 tgacaggcat tattcttgct gtctttgctc tgctggcaat cactcgcggt cttcatggcg
    19381 tcgcccggct catgcagggg tttgtcccgt tgatggcgat aatctgggta ctgaccagcc
    19441 tggtaatatg cgtaatgaat atcgggcaac ttccccacgt catttggtct atttttgaga
    19501 gtgcttttgg ctggcaggaa gcggcaggcg gcgcggcggg atatacctta agccaggcga
    19561 ttactaacgg ttttcagcgc agtatgtttt ccaatgaggc gggaatgggg tcgacgccaa
    19621 acgcggcagc ggcggctgcg tcctggcctc cgcatcccgc agcgcaagga attgtccaga
    19681 tgattggcat ttttatcgac accctggtca tctgtacggc aagcgccatg ctgatattac
    19741 tggcgggtaa cggcacaacc tacatgccgc tggaaggtat tcagcttatc cagaaggcga
    19801 tgcgggtact catgggttcc tggggtgctg agtttgttac cctcgtggtt attctgtttg
    19861 ccttcagctc catcgttgcc aactacattt atgccgaaaa caatctcttc ttcttacgcc
    19921 tgaacaaccc taaagcgatc tggtgtctgc ggatctgcac cttcgcaacg gtcatcggcg
    19981 gcaccttgct aagtcttccg ctgatgtggc aactggcaga tatcataatg gcctgcatgg
    20041 ctattaccaa tttgaccgcc atattactgc tctcgcctgt ggttcatacc attgccagtg
    20101 attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg
    20161 atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag taatcacaac
    20221 tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg
    20281 atatgctgat tcttatttca cctgcgaaaa cgctcgatta ccaaagcccg ttgcccacca
    20341 cgcgctatac gctgccggag ctgttagaca attcccagca gttgatccat gaagcgcgga
    20401 aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg gcgggtatca
    20461 acgccgctcg ctttcatgac tggcagccag atttcacgcc ggagaatgcc cgccaggcga
    20521 ttctggcatt caaaggtgat gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg
    20581 atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactgcgcc
    20641 cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag aatgcccgag
    20701 ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg
    20761 cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt aaatcggtga
    20821 agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag aagaacggca
    20881 aatttaagat catcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta
    20941 ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct
    21001 ttgatgaagc ttcctccagc aacggcgaac tggtgtttaa acgctacgag cagcgttaag
    21061 tttgcagtca atgccggatg gcaatccggc attgatattt agcgatgatg tttgtcaggc
    21121 cccggacgat gatcgccacg atgatcattg tgcttgttat ggcgcggcga tggcggcggt
    21181 ccatatgggt gccagcgatt gcctcgccat tcataatgtt gtttccacca gccgtggtcg
    21241 cgccagtgac cgccatccca gtaatagcca cgattatcac gatcgcctat ctgtaatttt
    21301 actgacggga ctaacgtcat ttccgcagcc tgcgcagcca tgggagcgac cagaaccagg
    21361 gaaagtgcga gtacgataga ttgcatcttt ttcacaggtt actcctttta tcattgcccg
    21421 ttatgggtcg atatccggag attacgcgat gagaacggca gttgttattc tccgcaatcc
    21481 taatctctaa gttaccgcat ttattgagca tttctccttt ttccctgtcg tttttctcca
    21541 taatttcttc ataaaaaagc cgggctgcat aaaagcaaac ccggcctgat tgagataatg
    21601 aatagatatt actgatgatt catcatcaat ttacgcaacg cagcaaaatc ggcgggcaga
    21661 ttatgtgaaa gcaagggtaa atcggcacgt tctgccagct cttttggcag atccaacgtt
    21721 tcaccgagaa tcgcttccac gctctcttta aatttcgccg gatgcgcggt gccgaggaac
    21781 aagccatatt cgcctggatg caactggtca cgcagtgcac gatacgctac ggcagcgtgc
    21841 ggctccgaag tgtagcccag ttcttttaac tcacgcattg tctgttgcgt ggtttcatca
    21901 tccacggctg cataacccag ctctttcagt tgccagattt tgcggcggaa caactcttcc
    21961 acacgcggcc agttgttcgg ctgactcacg tccatcgcgt tggataacgt cgcctgagtc
    22021 gctttgggtg accactgacc gtcgtgcagg aaacgtggca cggtatcgtt cacgttggtc
    22081 gcagcaataa aacgtttcac cggcagaccg agtgacttcg ccagcagacc cgccgtcaaa
    22141 tcgccgaagt ttccgcttgg caccgagaca accagctggt tgcgcgcctc ctgcggcagc
    22201 tgcgcaacag cttcaaagta gtagcaaatc tgcgccagca aacggctgat gttaatcgag
    22261 ttagctgagt ttagccccag cgccactttc agttcttcat catcaaacgc ctgcttcacc
    22321 agcgcctgac aggcatcgaa atcgccgtcg atggcaacag tttcaatatt gccgcccaat
    22381 gtacagaaca gtttttcttg cagtggactg attttgcctc gtggatagag gataaccact
    22441 ttcacattcg gcaaaccgta gaaagcatga gccactgccg ctccggtatc accggaggtc
    22501 gcggtcagaa tggtcactgg cttatcgccc gcaatatggg tcagcatttg tgccataaag
    22561 cgaccgccga aatctttaaa tgccagcgtt ggcccgtgga acaattccag acaaccgaca
    22621 tcgctttcaa cattggcgac cggagccggg aagacaaacg ccgcgcgtac gcgctcttcc
    22681 aggatttcct gcgggatttc atcaccaata aacgccgaga ggatcttcgc actgcgggtg
    22741 acaaaatcca gcttcagcat ctcatcaatt tcagtcaggc tgaattccgg caggtcgtgc
    22801 gggaaaaaca gcccctgatt tttgcccaac ccctgggtta cggcttgcgc aaagctgacc
    22861 tgctcattgt gatctttcag attgtagagt ttcatttagt tttccagtac tcgtgcgccc
    22921 gccgtatcca gccggcaaat atgaacaaaa ccttcctgat tttgcaggta gttcttaccc
    22981 aaccagtcgg caacgcgctg ggcggtatcc ggcttgtcac acagagcgaa caaagtcggg
    23041 ccggagccgg agataccgct cgctaccgcg ccgatttccg cgaccgcctg ccgcgcctgc
    23101 cggaagcctg gcagtaaccg ttcacggtag ggttcagcga taacatcttt catcagcttc
    23161 gcggcaagct caggctgacg ggaatagcag gcgtgaataa agcctgccag atgtcgcccg
    23221 tgcgcaatgc aatcctggcg gcgatactgc gccggtaaaa tagccctggc ttctgccgtc
    23281 gagactttaa tccccggata cgccagcacc cacagccact catcaaaccc tggcacttgc
    23341 tggctgatga tgtcgttttc ttcgatcatc aactgcatac caccgagaaa acacggtgcc
    23401 acgttgtcgt aatgaatgct gccggagata cgcccttcca gctcgcccat caaagccagc
    23461 aaacgggtgt cattaagcgg cttgccgcag tgttcattca tcgccatcag cgccgcgacc
    23521 actgagcagg cgctggagcc taagcccgag ccgatcggca tattcttttc cagggtcatc
    23581 gccactggaa tttgcttacc cagctcctgg caaaaacgct cccaacactg ataaacgata
    23641 ttttcccgag gttctgacgg cagcttatcg gcaaagcgtc cgaggttatt gagactgaat
    23701 gtctctgccg cctcaaccgt gactacatct ccgagcaatg caccatcaac gggtgtcacc
    23761 gccgccccga gcacatcgaa cccgacgctc atattggcac tggaagccgg ggcataaact
    23821 ttaaccatgt cagactccta acttccatga gagggtacgt agcagatcgg caaagacacc
    23881 ggcagctgta acgtcattgc ccgcaccata tccgcgcagc accaacggca gcggctgata
    23941 atagtggcta tagaaggcca gggcgttttc gccgtttttc actttgaaca gcggatcgtt
    24001 gccatccact tcagcaatct tcacgcggca gacgccatct tcatcaatat tgccaacata
    24061 gcgcaaaact tttccttcat cacgggcctt cgccacacga gcggcaaaga gatcatcgag
    24121 ctgtgacaga ttcgccataa aagcggcaac atcaccctcg gcgttaaact cagcgggtag
    24181 cacaggttca atttcaatat ccgccagctc cagttcacgt cccgtttcac gagcgagaat
    24241 caatagttta cgcgccacat ccataccaga aagatcatct cgcggatccg gttcggtata
    24301 acccatttcc cgcgccaacg tggtcgcctc ggagaaactc atgccttcgt ctaacttgcc
    24361 gaagatataa gaaagcgaac cagaaagaat gccggagaac ttcatcaatt catcacctgc
    24421 attgagcaga ttttgcaggt tctcaataac cggtaatcca gccccaacgt tggtgtcata
    24481 gaggaattta cgccgcgatt tttccgccgc atgacgcaac agatggtagt aatccatcga
    24541 cgaggtgtta gcctttttgt tcggcgtgac gacgtggaaa ccttcgcgca ggaagtcggc
    24601 atattgatcc gccactgcct ggctggaggt gcagtcaaca atgaccgggt tcagcagatg
    24661 atattctttc acgaggcgaa ttaagcgccc gagattaaac ggctctttgg cttgcgccag
    24721 ttcttcctgc cagttttcca gattaaggcc atgtacattg gtgagcagag ccttcgagtt
    24781 ggcaacgccg cagacacgta agtcgatatg tttattcttc aaccagcttt gctgacgctt
    24841 cagttgctcc agcagcgcac cgccaacgcc accgacgcca atcacaaaca cttcgataac
    24901 ctgatcggta ttgaacagca tctgatgagt aacgcgcacg ccagtggtcg catcatcgtt
    24961 atttaccacg acagagattg agcgttcaga agatccctga gcaatggcga caatgttgat
    25021 attggcgcgg gccagcgcgg caaagaattt cgccgagatc ccacgcaagg tgcgcatacc
    25081 atcacctacc accgagataa tggccagccg ttccgtcacc gccagcggct ccagtaagcc
    25141 ttctttcagt tccaggtaga actcttcctg cattgcccgt tcagctcgca cacagtcgct
    25201 ttgtggaacg cagaaactga tactgtattc ggaagatgat tgcgtaatca gcaccacgga
    25261 aatacgggcg cgtgacatcg ctgcaaagac gcgcgccgcc atgccgacca tccctttcat
    25321 ccccggacca gaaacgctga acattgccat gttattcaga ttggaaatgc ccttgaccgg
    25381 taattcgtct tcatcacggc tggcaccaat gagcgtacct ggtgcttgag gatttccggt
    25441 atttttaatc aggcaaggga tctggaactg ggcgatgggg gtaattgtgc gggggtgaag
    25501 aactttagcg ccgaagtagg aaagctccat cgcttcctgg taggacatcg acttcaacaa
    25561 cctcgcatcg ggcacctgac gcgggtcgca ggtatagacc ccgtcaacgt ccgtccaaat
    25621 ctcgcaacaa tcggcgcgta aacaggcagc cagcaccgcc gcggagtagt cggaaccgtt
    25681 acgtccaagc accaccagtt cgcctttttc attaccggcg gtgaaacctg ccatcaacac
    25741 catgtgatca gccggaatac gacttgccgc aatacggcgg gtggactcag caatatcgac
    25801 ggtagattcg aggtaatgcc ccactgccag cagtttttcg accggatcga taacggtaac
    25861 gttgtgacca cgcgcttcta acacgccggc cataatggcg atcgacattt tctcgccacg
    25921 gcaaatcagc gcagcgttga tgctatccgg gcactgcccc aacaaactaa tgccatgcag
    25981 gacatgtttt atttgggcaa attcctggtc gacgaaagtt ttcagttgcg ccagcgggaa
    26041 tcccggctga gcggcggcga gtcccgtcag aagttcggca aaaatacgtt cggcatcgct
    26101 gatattgggt aaagcatcct ggccgctaat ggttttttca atcatcgcca ccaggtggtt
    26161 ggtgattttg gcgggggcag agaggacggt ggccacctgc ccctgcctgg cattgctttc
    26221 cagaatatcg gcaacccgca gaaaacgttc tgcatttgcc actgatgtac cgccgaactt
    26281 caacactcgc atggttgtta cctcgttacc tttggtcgaa aaaaaagccc gcactgtcag
    26341 gtgcgggctt ttttctgtgt ttcctgtacg cgtcagcccg caccgttacc tgtggtaatg
    26401 gtgatggtgg tggtaatggt ggtgctaatg cgtttcatgg atgttgtgta ctctgtaatt
    26461 tttatctgtc tgtgcgctat gcctatattg gttaaagtat ttagtgacct aagtcaataa
    26521 aattttaatt tactcacggc aggtaaccag ttcagaagct gctatcagac actctttttt
    26581 taatccacac cgagacatat tgcccgttgc agtcagaatg aaaagctgaa aaatacttac
    26641 taaggcgttt tttatttggt gatatttttt tcaatatcat gcagcaaacg gtgcaacatt
    26701 gccgtgtctc gttgctctaa aagccccagg cgttgttgta accagtcgac cagttttatg
    26761 tcatccgcca ctgccagagt cgtcagcagt gccatggctc gttcgcgtaa agcttgcagt
    26821 tgatgtttgt ctgccgttgt gtcacttttc gccggttgtt gtattaatgt tgctaattga
    26881 tagcaataga ccatcaccgc ctgccccaga ttgagcgaag gataatccgc caccatcggc
    26941 acaccagtaa gaacgtcagc caacgctaac tcttcgttag tcaacccgga atcttcgcga
    27001 ccaaacacca gcgcggcatg gctcatccat gaagattttt cctctaacag cagcaccagt
    27061 tctactggcg tagcgtagta atgatatttc gcccgactgc gcgcagtggt ggcaacagtg
    27121 aaatcgacat cgtgtaacga ttcagccagt gtcgggaaaa ctttaatatt atcaataata
    27181 tcaccagatc catgtgcgac ccagcgggtg gctggctcca ggtgtgcctg actatcgaca
    27241 atccgcagct cgctaaaccc catcgttttc attgcccgcg ccgctgcccc aatattttct
    27301 gctctggcgg gtgcgaccag aataatcgtt atacgcatat tgccactctt cttgctcaaa
    27361 taaccgcgaa ccgggtgacc actgtcaact tattacgcgg tgcgaattta caaattctta
    27421 acgtaagtcg cagaaaaagc cctttactta gcttaaaaaa agctaaacta tttcctgact
    27481 gtactaacgg ttgaattgtt aaaaaatgct acatatcctt atgtttactt aggataattt
    27541 tataaaaaat aaatctcgac aattggattc accacgttta ttagttgtat gatgcaacta
    27601 gttggattat taaaataatg tgacgaaagc tagcatttag atacgatgat ttcatcaaac
    27661 tgttaacgtg ctacaattga acttgatata tgtcaacgaa gcgtagtttt attgggtgtc
    27721 cggcccctct tagcctgtta tgttgctgtt aaaatggtta ggatgacagc cgtttttgac
    27781 actgtcgggt cctgagggaa agtacccacg accaagctaa tgatgttgtt gacgttgatg
    27841 gaaagtgcat caagaacgca attacgtact ttagtcatgt tacgccgatc atgttaattt
    27901 gcagcatgca tcaggcaggt cagggacttt tgtacttcct gtttcgattt agttggcaat
    27961 ttaggtagca aacatgcaga ccccgcacat tcttatcgtt gaagacgagt tggtaacacg
    28021 caacacgttg aaaagtattt tcgaagcgga aggctatgat gttttcgaag cgacagatgg
    28081 cgcggaaatg catcagatcc tctctgaata tgacatcaac ctggtgatca tggatatcaa
    28141 tctgccgggt aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc
    28201 gttgatgttc ctgactggcc gtgacaacga agtcgataaa attctcggcc tcgaaatcgg
    28261 tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa
    28321 cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag
    28381 ctacaagttc aatggttggg aactggacat caacagccgt tcgttgatcg gccctgatgg
    28441 cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc
    28501 aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg accggccgtg agctgaaacc
    28561 gcacgaccgt actgtagacg tgacgatccg ccgtattcgt aaacatttcg aatctacgcc
    28621 ggatacgccg gaaatcatcg ccaccattca cggtgaaggt tatcgcttct gcggtgatct
    28681 ggaagattaa tcggctttac caccgtcaaa aaaacggcgc tttttagcgc cgtttttatt
    28741 tttcaacctt atttccagat acgtaattca tcgtccgttg taacttcttt actggctttc
    28801 attttcggca gtgaaaacgc ataccagtcg atattacggg tcacaaacat catgccggcc
    28861 agcgccacca ccagcacact ggttcccaac aacagcgcgc tatcggcaga gttgagcagt
    28921 ccccacatca caccatccag caacaacagc gcgagggtaa acaacatgct gttgcaccaa
    28981 cctttcaata ccgcctgcaa ataaataccg ttcattatcg ccccaatcag actggcgatt
    29041 atccatgcca cggtaaaacc ggtatgttca gaaagcgcca gcaagagcaa ataaaacatc
    29101 accaatgaaa gccccaccag caaatattgc attgggtgta aacgttgcgc ggtgagcgtt
    29161 tcaaaaacaa agaacgctat aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc
    29221 cggtcagtta attggtattg atcggctggc gtcgttactg cgacgctaaa cgccgggaag
    29281 ttttcccagc cggtatcatt gcctgaagca aaacgctcac cgagattatt agcaaaccag
    29341 ctgctttgcc agtgcgcctg aaaacctgac tcgctaactt cccgtttggc tggtagaaaa
    29401 tcacctaaaa aactgggatg cggccagttg ctggttaagg tcatttcgct attacgcccg
    29461 ccaggcacca cagaaagatc gccggtaccg cttaaattca gggccatatt cagcttcagg
    29521 ttctgcttcc gccagtcccc ttcaggtaaa gggatatgca cgccctgccc gccttgctct
    29581 aagccggtgc cgggttcaat ggtcagcgcc gttccgttaa cttcaggtgc tttcaccaca
    29641 ccaataccac gcgcatcccc gacgctaatc acaataaatg gcttgcctaa ggtgatattt
    29701 ggcgcgttga gttcgctaag acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg
    29761 tgccagacct gaccggtata aatccctatc ttgcgttctt ccacgttctg attgccatca
    29821 accatcaatg actcaggtaa ccaaaaatgg ataaaacttc gtttccgctc cacggtttta
    29881 tcctcttcct gcaccgtata aagctcggtc acaggaatag cgatgagcgg cccaacgagt
    29941 ttttgcggcc cgctggtact ttggcgaatc gcatcttcca catcgctacg gtaatcagca
    30001 cgttcgacaa tcacctgccg aatcagcatt atcggaatca acaacagcaa tactgcacca
    30061 aacagggtgg tcattttcca gaacaggggg gatttcaaca tagtcttctc ctttgcaatg
    30121 gcagcaggat acgaaggcta tgtgggaaga atttgaagct atgtgaagtg acggtgaagt
    30181 cgaagcgaag ccagcacgcc accttcctgc acattgcgca gcgtgacttc gccgttaaac
    30241 aaacgggcga cctcactgac gaacgccaac cccagaccgc tgcttttttg cccatttgca
    30301 cgaggcaaag agtaaaagcg ttcaaaaata cgtgaaagcg cgtagtcagg aataccacta
    30361 ccggtatcca gcaccttaag cgtgacgtgt tcctgctcca cttcggcgct tagcgtgatg
    30421 caaccgctct cgggggtaaa atcgatggcg ttatccagta aattccccag agcctgctcc
    30481 agtaacgccg gttcagcagc aacgttaacc tcggtgggcg taacatgtag agtgatgttt
    30541 ttttctgcca actgcacggt gcgcgcttcg ctgacgcggc ggaataatgc cgccacatca
    30601 acagcagtta gaacgacttc ctgacgattc tccagtcttg cctggcgtag taacgtttct
    30661 accaatgcct gcatacgcgc attttgcgtc agaatgttgt cagtaaaacg agccaccact
    30721 tccggcggcg gaccttcgcg taaaatttcc gccgctccac gaatcgccgc cagtgggctt
    30781 tttagctcat gagttaatgc gtaaacatac tgctcaatat agtttttccc ttccagcttc
    30841 acgcgcatac tttccagcgc ctgcgcgagt ttacgcagct cgctactacc gagatcgggg
    30901 agaggaacgg gcttattgtc agtgacggaa tcagcatagc gagtgagccg ggcaatggag
    30961 cggttgatcc accaaaccat gcctgcgcca atcaccagtg caatccccaa caaaatggcg
    31021 ctggcccata aaattcgccg ctcgctgcgt ttaatgaccg gagccatcgc cgcgttcggt
    31081 ttgcctacgc tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac
    31141 atcaccgaac tttcgggatc ggtaggattt tgcaacgtgc tgcgcgcacc atactgacca
    31201 cgcaacgtta gccagacgtc attccagcgc gaataatcct gtccaacggc tttatttgcc
    31261 gaatcgaaca atactttgcc ctgcgcatcg gtcatataga catgatattc attgcgcact
    31321 ttgttaatgc caccgatatt ggcgcgaaac gggcgatgct gtagctgatt aaacgcctgc
    31381 gccagttgcc catgcgttgt gtccccagag agcaaatcgg gacgcgccag ctccgccagc
    31441 aacgttgcgg tgtcgatcaa cgtcccctcc gttgctcttc gcacgcccgg tttaacttct
    31501 ttgacaaaaa tggccagtac gaaccaagct gccaccgcca ccagtaaaaa atagcccagc
    31561 aacaaccgca tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag
    31621 tattaatcgg tgaaagatcg gggttgatgg cgcgcagctt ggcacgcagc gttttaatgt
    31681 gggtatcgac ggtgcgatcg taggtgtcct gcgcatcttc ccacacgcta tccatcagtt
    31741 gctggcggga ccagacgcgg cctggcgact tgagtaacgt cttcagcaat aaaaactcat
    31801 accgcgtcag cgttaatggc gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt
    31861 caaaatgacc aatacggatg acgggagacg gcgtcgagaa cttcttcacc cgacgcagta
    31921 aggtgcgcac cctggcgcac acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag
    31981 cgccaatttc cagcccaagc aggcgatcga cctcttcact tcgggcggtc aggaacagta
    32041 caggtaacgc cggatggagc gtcagtaact ggcggcacaa ttcaaagcca ctgatatcag
    32101 gcaaaccaac gtcgagaatc atgacatcgg gaacctgctg gcgagcttta tccagcaccg
    32161 gcaagcctcg ctcaaagacc tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg
    32221 tgtcggctat cccttgctca tcttccacta accagaccgt ttcccgttgc ataaatcgcc
    32281 tctgttattg ccgccacggc atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt
    32341 ctacaacttt gtcggagtaa gccagataag cgagcgcgtt gcgtttagcg tcataaaagc
    32401 gcacgacctg taacgactta aagaccaggg acgtgcgttt tttgaatact acctcgccct
    32461 gagctttgcc gtttttaata cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa
    32521 tggccgcatc ggaggtatct tctgccaggc ccaatccccc tttaatacca ccggttttcg
    32581 cccgactcac ataacaggtg acatttttca catcgggatc gtcaaaggct tccacaacaa
    32641 ttttgtgatc cgggccgatc attttaaata cggtgtcgac cgaaccaatc tcttctgcat
    32701 gagccaatgg ccccagcatt atcaggctta aagacaggat caaatgcttg tatttcatat
    32761 tgttaccatt tttatctatt acacgagcaa ttagttcgtc acggtaaata acatattgat
    32821 taagaacaaa atctcaatac ttttatttcc gtcaagccct aaaacatact ctactaagaa
    32881 aaaaacactg aatgctaaaa cagcaaaaaa tgctattatc caattacctg atgtcaggtg
    32941 ctcgttgttg aaaggatgag gatattttat ggatcaggcc ggcattattc gcgacctttt
    33001 aatctggctg gaaggtcatc tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc
    33061 aggttattcc aagtggcact tacagagaat gtttaaagat gtcactggcc atgctattgg
    33121 cgcgtatatt cgtgctcgtc gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg
    33181 tccgattctg gacatcgcgc tgcaataccg cttcgactct caacagacat ttacccgcgc
    33241 attcaagaag cagtttgccc agactcctgc actttaccgc cgttctcctg aatggagcgc
    33301 ctttggtatt cgcccgccgc tacgtctggg tgaattcact atgccagagc acaaatttgt
    33361 caccctggaa gatacgccgc tgattggtgt tacccagagc tactcctgtt cgctggagca
    33421 aatctctgat ttccgccatg aaatgcgtta tcagttctgg cacgattttc tcggcaacgc
    33481 gccgaccatt ccgccggtgc tctacggcct gaatgaaacg cgtccgagtc aggataaaga
    33541 cgacgaacaa gaagtattct ataccaccgc gttagcccag gatcaggcag atggctatgt
    33601 actgacgggg catccggtga tgctgcaggg cggcgaatat gtgatgttta cctatgaagg
    33661 tctgggaacc ggcgtgcagg agtttatcct gacggtatac gggacgtgca tgccaatgct
    33721 caacctgacg cgccgtaaag gtcaggatat tgagcgatac tacccggcag aagatgccaa
    33781 agcgggagat cgcccaatta atctgcgctg tgaactactg attccgatcc gtcgttaacg
    33841 ctgcagctca tctaatgcag gggcgtccag atgcgagatg tcccctgcgg tttcaacgac
    33901 ccagccagac gccagccaca ggctttcctg ataatccacg cgcgaaatag aacagttacg
    33961 cagacgtaag cggcgctctg cccatgctgg taatccgaga atcgtactca ccagacatcc
    34021 cagtgcaata ccatggctca ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga
    34081 ttccagtgcc gcattgacgc gatcgctgag ctcctgcatt gactcgcctt caggaatacg
    34141 cccgtcaacg gtgccattga ccagctgccg acgccagttc tcttcttctt cggtgagtga
    34201 atcgatattt cttgtttcca gtacacccat atttaattca cgcaggcgag aatcaaagat
    34261 gatatcacag ccgcaggcct gggcgatgat ttccgccgtg cgccgggtcc gtcctaaatc
    34321 gctactgatg atatgcgtaa tgccaagctc tttggcacgg gttgccacct gcatcgcctg
    34381 ttgctcacct ttggcggtca gcgggctgtc agactggccc tgaatacgtc gctcggcgtt
    34441 ccactgcgtt tcaccgtggc ggactaggta tacctgtaac atgctttttt tccgtatact
    34501 gctgtaacgt taattttctg agtaatgctg attatgcacc aagttgtctg tgcgaccacc
    34561 aatcccgcta aaattcaggc cattctgcag gcatttcacg agatcttcgg cgaaggatcc
    34621 tgccatattg catccgttgc cgtcgagagc ggtgtaccgg aacaaccctt tggcagtgag
    34681 gaaacgcgcg ctggcgcacg aaatcgggta gcaaatgccc gccgtttaca tccagaggct
    34741 gatttttggg tggcgattga agcaggcatt gatggcgaca gcaccttcag ttgggtggtg
    34801 attgaaaacg ccagccagcg cggcgaggcg cgttcggcaa ctttgccgtt acctgcggtg
    34861 attctggaaa aagtgcgtga aggcgaggcg ctcggtcccg tgatgtcacg ttataccggt
    34921 attgatgaga ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaacttacc
    34981 cgcgccagcg tctatcacca ggcggtgatc ctggcgctga gtccgtttca taatgcggtg
    35041 tatcagtgat ctttcagcaa cacctcttcc agccactggc gcagctccac gggcgcggct
    35101 ttcaggctgt tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac
    35161 tcacgctggc tcatttcgcc gcgcaacagc tcttcgacaa tacgcacgcg agtccccaac
    35221 gcttcgcgct catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg
    35281 taggcattct taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc
    35341 gctgctgaat agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa
    35401 aatgcctccc ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc
    35461 agtaacgacg tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc
    35521 ggtagtaagc gtcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa
    35581 tactctcgac aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca
    35641 cccgtccagg tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct
    35701 gataaacata ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt
    35761 gcccagggct gctataacca ggaatagaga acatcttcac cgtatgggtc gccgtgcccg
    35821 gcataatctg catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc
    35881 tctcctgacg ggcaatcgcc atcgcatagc tttgcgggat ctccttccca ctggtgtaac
    35941 gtttgaaaag atcgttgtag gccagcggga atctctcttc cagatgatcc caaagcttcc
    36001 cggcgatcgt tgcctgaacg ctaagttccc accattggtt attgaaagca taccgagcca
    36061 gttgcgcctg ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg
    36121 tattatcgag attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca
    36181 gggcgctgtc aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc
    36241 gttgtgctgc aaccatcggg tagaaaccac gctgttgcat gagttgatgc aaaatctctt
    36301 ttgcttcagc ttcacgtccg cgttccagca gtaaatccgc ctgccagtaa cgccattcat
    36361 ctttctcttt cgcctccatc ggcaaacgcg ccagccaggt attcaggccg cgacgatcgc
    36421 cggtgccaag cgccattcgc acgcgccgtt caataagcga agtagattgc gaacgcataa
    36481 tggcgtcatc gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg
    36541 cgacgatatc gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg
    36601 acgggatcat cagccgtgca ttttcagcat cctgccgcgc cacactggca aacgccaccg
    36661 ccgccatttg acgggtaaaa tcggtcgctc cggttgtacg cgcgaaggtc agtaccgtat
    36721 tagggttgtt cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct
    36781 gccctgccag cactgttacg agtccagtgt tacccgcttt catcgccaga cggatacgct
    36841 ctaaatacgc cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac
    36901 aggcgttagg ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt
    36961 cactctgccc ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg
    37021 gcttttccgg gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga
    37081 cgaaacgaga ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag
    37141 tgaccgtcac cgccggttga ttcatcaggt catcggtgat ctgacggtat tccaggtagg
    37201 gataaagcgg ataatctttc agtccaggca tcatttgttc gaccacatcc atttgtcgat
    37261 tatcccaggc ctgcttgatt tgggcgtagc ggctacgctg ctcatccagt gagtcggctc
    37321 gcgccacgct gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt
    37381 gtttggcttt ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc
    37441 gcgtaataga tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt
    37501 acttttatac tctaaataat tcgagttgca ggaaggcgat aagtgagtga gtccccagga
    37561 gcttacagaa gtaagtgact ggggtgagcg agcgtaagcg cagcacatgc aacttgaagt
    37621 atgacgagta tactgcgagg gtgatcgacc ggctgggatt agataaggaa acaggctaca
    37681 cttcgccttt gaaaactcat catcacgata aacatagagg cgaagtccaa cgtggctcaa
    37741 ttcgtttata ccatgcatcg tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa
    37801 aacatctctc tgagtttctt ccctggggca aaaattggtg tcctgggtct gaacggcgcg
    37861 ggtaagtcca ccctgctgcg cattatggcg ggcattgata aagacatcga aggcgaagcg
    37921 cgtccgcaac cggatatcaa gattggttat ctgccgcagg aaccgcagtt gaacccggaa
    37981 cacaccgtgc gtgagtccat tgaagaagcg gtttctgaag tggttaacgc cctgaaacgc
    38041 ctggatgaag tgtatgcact gtacgccgat ccggatgccg attttgacaa actggccgct
    38101 gaacagggcc gtctggaaga gatcattcag gctcacgacg gtcacaacct gaacgttcag
    38161 ctggagcgtg cggcggatgc gctgcgtctg ccggattggg acgcgaaaat cgctaacctc
    38221 tccggtggtg agcgtcgtcg cgtggcgttg tgccgcctgc tgctggaaaa accagacatg
    38281 ctgctgctcg acgaaccgac caaccacctg gatgccgaat ccgtggcctg gctggaacgc
    38341 ttcctgcacg acttcgaagg caccgttgtg gcgattaccc acgaccgtta cttcctcgat
    38401 aacgttgcgg gctggatcct cgaactggac cgcggtgaag gtattccgtg ggaaggtaac
    38461 tactcctcct ggctggagca gaaagatcag cgtctggcgc aggaagcttc acaagaagct
    38521 gcgcgtcgta agtcgattga gaaagagctg gagtgggtac gtcaaggtac taaaggccgt
    38581 cagtcgaaag gtaaagcacg tctggcgcgc tttgaagaac tgaacagcac cgaatatcag
    38641 aaacgtaacg aaaccaacga actgtttatt ccacctggac cgcgtctggg cgacaaagtg
    38701 ctggaagtca gcaatctgcg taaatcctat ggcgatcgtc tgctgattga ctccctgagt
    38761 ttctcgatcc cgaaaggggc gatcgtcggg atcatcggtc cgaacggtgc gggtaaatcg
    38821 acgctgttcc gtatgatctc tggtcaggaa cagccggaca gcggcaccat cactttgggt
    38881 gaaacggtga aactggcatc ggttgatcag ttccgtgact caatggataa cagcaaaacc
    38941 gtttgggaag aagtttccgg cgggctggat atcatgaaaa tcggcaacac cgagatgcca
    39001 agccgcgcct acgttggccg ctttaacttt aaaggggttg atcagggtaa acgcgttggc
    39061 gaactctccg gcggtgagcg cggtcgtctg catctggcga agttgctgca agttggcggc
    39121 aacatgctgc tgctcgacga accaaccaac gacctggata tcgaaaccct gcgcgcgctg
    39181 gaaaacgccc tgctggagtt cccgggctgc gcgatggtta tctcgcacga ccgttggttc
    39241 ctcgaccgta tcgccacgca catcctggat taccaggatg agggtaaagt tgagttcttc
    39301 gaaggtaact ttaccgagta cgaagagtac aagaaacgca cgctgggcgc agacgcgctg
    39361 gagccgaagc gtatcaagta caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg
    39421 cgcgccagca tcgcatccgg tattagttaa atgattaatg ggaggcaagt tcccggaaaa
    39481 agtcaggatt ttctttaatt gtggcaagta cttttgcagc caatcccgta ggatttcttc
    39541 gcttcgattc ccagctttta atggtatcca cactggttcc cagagctttt gccatctcac
    39601 tttgcgaaac gttgagttgc tcacgaatag ctttaacatc cgcaatttcg tagcgggtca
    39661 cccgtgcagg ttcttttaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca
    39721 actcatcaaa aaaactcatt ttatatctcg cctttgagca gtttcgtcaa cttctttagt
    39781 tgagcttttt ccgtatctgt cagactatct ttggcatttt tcggataggc cataatcaaa
    39841 taaataatct cttccgtcgc cagaaagtaa attattctga cactgccact ttttccctgt
    39901 gaacccgcag ccattcggac tttacgcaat ccaccagtct gctgaatgag atcaccttta
    39961 tcgggagttc cgatgagttc tttttgtaat tcctttaatt catcatcggt tgcaatctgc
    40021 ttgatctgac gcgtaaacat tggcgtctct ataaatatta tcgccttacc cattacccct
    40081 ccctggaagg tacattgtac accaaaatgg agtactaagt acacttttga atcagtaatc
    40141 ccactcaaca tcttataaag atgattatct ttgctccccc atcatctccc gcaccagctc
    40201 cacgcagcgc aggaaacggc tgtcataatc gtcttcttca acccgcacaa attcgatatt
    40261 gttctcttcc agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc
    40321 gaggctgcgt aaaccatccg ccacccaagg ggtgttgttc tccagcaaga taaccagatc
    40381 aaaacggtat tcatcgatca gcgcctgcac gaacggatgc tcccgccctt cgtacttttt
    40441 gcagaacgcc tgggtagtga caaaatcagt gtcgataaac gccactttat tggcatattt
    40501 cactgcaaaa tcaatgtatt gtgcgtgtcc cagcgcgatt ttatcgtagt cggaatactg
    40561 caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat attcccacgc
    40621 actggtggta ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc
    40681 gccgccaagg atcgccacgg tacgcacaaa aaacggcttc acttcagtgg ggatatattc
    40741 ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtgcgttt
    40801 cggatcgacc agcaccgttt cgatccccag atgttccata tactgtggcg catcggcttc
    40861 ttccgaggtg tagatcaggt ccggctggat ccccttttca gccataaact ttttgatgcc
    40921 gttgctccat acatcccagc cgtgcggata tggctccatg ccctcttcat tgaaagcatg
    40981 aatacggata tttttctgat atttaaaggt ttgcaataac cagcgcagac gatccggcac
    41041 cgtgggctgc tgcgacatgg cgctgtcttc gaacaacgcg cggtcgcggg tatcgtcaaa
    41101 gcccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg
    41161 tccggtatgc agcgggtaga acttaccgaa tacgacacca atggttttct tctgtcgggg
    41221 aaattcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttaat
    41281 tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat cagcgacctg
    41341 ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc
    41401 tccctccgta tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta
    41461 acgccaaaaa tctgcatccc ttccggcgct tttttcggca cgttagccgc cggaacaatc
    41521 gcccggcgaa aaccgtgttt cgccgcttca gaaattcgtt cctggccgct gggcaccggg
    41581 cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgcgg cagcggtctg
    41641 tcgcgcaggc tggaaaccat cgccagcagt aacgctaagt cggcgctggt ttccgttacc
    41701 ttcacgccgc cgaccacgtt aacaaataca tcctgatcgg ccatttgcaa cccaccgtga
    41761 cggtgcagca ccgccagcag gattgccaga cggttttgct ccagccctac tgcgacgcgg
    41821 cgtgggttcg ccatcatcga gtgatcgacc agcgcctgga tctccaccaa cagtggacgc
    41881 gttccttccc acactaccat cactgagcta ccggaggtca cttcatcacc gcggcttaaa
    41941 aagatggcgg aagggttgct aacttcacgc agcccctgtt cggtcatcgc gaagacgccc
    42001 agctcattca ccgcgccgaa gcggttttta tggctgcgta aggtgcgaaa acgagagtcg
    42061 gcatcgccat ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc
    42121 agcgagccat ctttggttac gtgcccaacc atgacaatcg ccacgccgcg cgttttggcg
    42181 aagcgcgtca gataagccgc cgtttcacgc acctgcgcca cgctgccagg cgatgactga
    42241 acatccgcca tatgcatcac ctggatggag tcaattacca tcagcttcgg ttgctcttct
    42301 tcggcaatca ggcagatctg ctcgatgctg gtttccgaca gcatattaag attgtcagtc
    42361 ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccagtgaca
    42421 tacagcgttt tcatctgctg cgccagtttg cacagcgttt gcagcaacag cgtggatttc
    42481 cccgcacctg ggttaccgcc aatcagaatg gcgcttcccg gcaccacccc gccgcctaat
    42541 acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcctcaag gctgatgtcg
    42601 gaaagtttct ggacttttgc cactccggcg ctaccggcat agccactgag acgctcgtta
    42661 cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag
    42721 gcgctgcact gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg
    42781 cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatgacgg
    42841 gcaggcaatt acttctgatt caggctgcca gagaggatgc agaatacccc catcaggtca
    42901 gcgtgacgga tggtgacttc cgtcttttca ttcacttttg gcttggcatg gtaggcaatc
    42961 cccagccctg ccgctttgat catcggcagg tcattggctc catcgccaat cgccacggtc
    43021 tgcgccagcg ggatttcata ctcctgcgcc aggcgggtca gggttttcgc tttatattgc
    43081 gcgtcaacga tgtcgccgat cacattgccg gtaaatttac cgtccatgat ctccagttca
    43141 ttggctacca ctgctgtcag gcgcagcttg tcgcgcaggt attcagcaaa gaaagtaaag
    43201 ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagtac cagctgcgtt
    43261 aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct
    43321 ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc
    43381 cgttcggtta cctccgccac cctctcgccc gttccggcca gtttggcaat ttcatcaata
    43441 cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg
    43501 tgcgggattt tccccagcgg cgcgacatcc agctgcgctt cgtgggccag gcgcgtagcc
    43561 cgcgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca
    43621 acaatcacca tcgccgcgcc cagtttgctc tggtattggg tcagacgttg tttatccagc
    43681 ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca
    43741 tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac
    43801 caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat
    43861 gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga
    43921 agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc
    43981 ttgccttgtt agtggcgctg atgcagggag cgtcatggtt tagtcaaaac caccagcgac
    44041 agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg
    44101 ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg cattcaggcg atccttgatc
    44161 agttaacgga tgaaagccgt atcctcgacg cgggcgtgta tgacgaacaa ggcgatctta
    44221 tcgcccgttc tggcgaaagc gtcgacgtgc gcgaccggtt ggcgctcgac ggcaaaaaag
    44281 cgggcggcta ttttaaccag cagattgtcg aaccaattgc cgggaaaaac ggaccgctcg
    44341 gctatctgcg ccttacgctc gacacccata cgctcgccac cgaagcccaa caggtggata
    44401 acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga
    44461 cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg
    44521 ccagtaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa
    44581 gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt
    44641 ttaacctggc ggtagaggag tgtatttttc gccagatgcc ggctacgcag cgcgttctgt
    44701 ttctctggcg caatgccgac acggtagtga ttggtcgcgc gcagaacccg tggaaagagt
    44761 gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg
    44821 gcgcagtgtt ccacgatctc ggcaatacct gttttacctt tatggctggc aagccggagt
    44881 acgataaaac catctccacg tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg
    44941 ccgaagcttc cgggcgtaac gatctggtgg tgaaaaccgc cgaaggcgat cgcaaagtct
    45001 caggctcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcact ttgctgctca
    45061 attccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga
    45121 aaggcattac ctcagtgcgt tcccgcgtga ccaatctgac cgaattgcta ccggggatca
    45181 cccatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat ggcgagcgcg
    45241 tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct
    45301 ttgcccgcca gagtagctgg gaatggaact tcggtcaggc tccggcattc tcgcatctgc
    45361 tggatgaacg ctttacctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata
    45421 tcacccgcgc ccaggtgttt accgacagtc tcaaccccgc gccactggaa gccctcgccg
    45481 ggcgactgca aggctgtctg taccgcgcgg atatgctgca acaggagtgc gaagcgctgc
    45541 tggttgactt cccggaacag gaaaaagagc tacgggagtt gtcagcgtgg atggcagggg
    45601 cggtaaggta actcgctgat taaaatatcc agggaaggaa atatgatcga tctggaaaac
    45661 caggaacggg agattatcaa tttaatgttc agccagggta tatcctggct aaccgccgtg
    45721 cgtatcaggc ataaactatc acttgctgaa gtaagtaaaa tgttaggtat ctccattaat
    45781 tctctaaaac agatcgaaaa gacggaacgg cttagttcaa acatcaaaag caaaatggca
    45841 ggaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat
    45901 aaatgaatag attcgtgaat agatacttga atggatttta ttttctatcc atatgaaaaa
    45961 taatagtaaa tctataaatc agttgctatt gatgaataaa tactaaaaag ccggagcagt
    46021 ctcccactcc ggcttcacac gacaatcgcc tttcagcaaa acacaattac tctttatcgc
    46081 ccagcagaac ggattccagc gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag
    46141 cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg gtcagggctt
    46201 tcgcgccaaa ttctgcagcg acgccgtaga taccagccgc ttccatttcc acgccgagga
    46261 tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac aggtcagcgg
    46321 agaacaggtt accaacgcga gcatcaacgc ccagtgcttt tgccgcgtct accgcgttac
    46381 gcaccatgtc gaagtcagcg atagcggcaa agtcatggtc tttaaaacgg atgcggttaa
    46441 ctttggaatc ggtgcaggca cccataccga taacgacgtc acgcagtttt acgtgcggca
    46501 gaacagcacc acaggaacct acgcggataa ttttcttcac gccgaaatcg gtgatcagtt
    46561 ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg gaaattttgc
    46621 ggcctttata agtaccggtg aagcccagca taccgcgaac gttgttcact tcacgggcat
    46681 cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc atcaaaacta
    46741 cgtcagcaaa atcgcccatt tctgcattaa tgtgtggagt agccatcgtt ttatcctttt
    46801 tgtgacatat taaaggcgga gtcactgctc cgcccaaatc catcagaaca tggctttgcc
    46861 atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat ccgcgaaggt
    46921 ttcacgatgc cccagcgaac ctggtttcac tttcgggccg taaaccagta ccggaatgtg
    46981 ttcacgcgtg tggtcagtac cggtccaggt cgggtcacaa ccgtggtcag cagtgagaat
    47041 cagaatatcg tcatcgcgca gcaaagacat cagctccggc agacggcggt cgaacagttc
    47101 cagacccgca gcataaccgg cgacgtcgcg acggtggccc caggaagagt cgaagtcaac
    47161 gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga tggtggcgtc
    47221 aaacagcgcg tccaggccgg tcgctttcac ttttttggtg ataccgcagt tggcatagat
    47281 gtctgcaatt ttaccgaccg aaaccacctg gccgtgtttt tcatcaacca gtttctgcag
    47341 cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac gctggaagtt
    47401 accggctttg tcgccgataa acggacgagc gataacacga ccaatgttgt agccgccgtt
    47461 ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac caaaggtttc
    47521 ttcgtggcaa gcaatctgga acacggagtc agcggaggta tagaaaatcg gcttgccggt
    47581 tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt ggcagttacc
    47641 gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt gcgggaagct
    47701 gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg cgatttccca
    47761 gtgaccagac ggggtgtctt taccggatga catttcgtgc gcccatgcgt acgcgccgat
    47821 aacttcagcg ttgccatcca ttcccgccgg aatgaaaccg gtagaacctt cgtgtgcttt
    47881 cgccagccca agacgggtca gatttggcag attgagcggg cctttacgac cgtggtcagc
    47941 ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga cgtcaccaaa
    48001 gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa taaatgcacg
    48061 tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag tataccgtta
    48121 ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg caagtttaat
    48181 tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg cgtggataac
    48241 tgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag taaagccgac
    48301 gctgtaatcg atggtgtcag atgcctgacg gcgtccgccg cccattgcaa ccactgccat
    48361 ccccagtgcg cgggtatcca tttcactgac aaacccttcg gtatcagcat agactgcttt
    48421 cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg tcgggccttt
    48481 ttgtgccgcg accatacgac caaagacttc tgccgcttta ccgttgtcca gcaccgcctg
    48541 caatttcgcg cgcgcttcgg cgtcattttt cgccagtttg ccggagatca gcatctccac
    48601 gcacagtgcc atcgtgacat caaacagacg cgggttacga tattcacccg tcaggaactg
    48661 caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct gattcatatc
    48721 ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa tcgcttcggc
    48781 aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacctactt tcacgtccat
    48841 caccagcgcg tccagacctt ccgccagttt cttcgccaga atagaggcag tgatcagcgg
    48901 gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat ccgccggagc
    48961 cagtgagctg gtctggccga taatcgccac gccgacgtct ttaataattt cgcggaaacg
    49021 gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga gcgtaccgcc
    49081 agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg ctgcgaccat
    49141 cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt tatcaacaat
    49201 cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc gcatagccat
    49261 ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca tcgcgagggc
    49321 ggcaatctgc ccttcggaga tagtgttgtc gcgaataccg ttgataaaga aacgaatttc
    49381 ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg cgagaaacaa
    49441 ggtaacctcc cgtggaaaag agacgaggta atcggggaac cggatgagca tcgctgcatc
    49501 atccggcaaa tcaagcctta gtagctgctg gcgctcttac cgtcgccgtg gcccagagct
    49561 ttcagcaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atcagcccag
    49621 tcagcaccga acagttcatc ggcgatggcg agatatttct gcgcatcttc cgcagtacgc
    49681 acgccgcccg ccggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    49741 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    49801 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    49861 tttagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    49921 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    49981 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    50041 gcgatttcga tgtcgtcgtt accgtgtggg aagttggtta ccgtagcgat acggatttcc
    50101 ggggtaccct gctctttcag cgttttgcga gcaattggga taaagcgagg atagatacag
    50161 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    50221 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    50281 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    50341 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    50401 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    50461 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    50521 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    50581 gcaacaacgt tgccagcaaa ttgtcaccag cccggtactt agcccggagc agaagcgcca
    50641 ttttctggca ctggaagcag aaaacaatct gccttaccca cagcttcctg ccgaagcccg
    50701 ccgcgcgctg gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    50761 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    50821 ggaaggcgcg aaagatcttg atgatgcact ctctctgctg accattcttt accaccacgt
    50881 accatcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    50941 tgttagaatt ctaacacaag acgagatcga tattcgaata aaacgtttct ggcgttacct
    51001 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    51061 tacccgcgcg atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    51121 tatctacgat cctgaaatca cccctgatga cctactgctg gaagtggcga agaacatctg
    51181 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    51241 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    51301 ggtacgcctt aacctgaaag ccattgccga gcgcagtgaa tcgctggatg acttctttac
    51361 acgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    51421 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    51481 ccctgaacgt tttgtgccaa tgtttggcat gtacggattg gcggaagcag ttaacttgct
    51541 gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg
    51601 ttatcgcatc agcgcacaac tggcggagtt tgtcgccaat acccccgtga aatatggctg
    51661 gcaaaaacgc gccatgctac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc
    51721 tggcgcacgc ttgccgtatg gtgatgagcc agatccgatt acccatctgc aaactgtcgc
    51781 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    51841 caaacgtaat ccgcaggcgc tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    51901 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg
    51961 tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    52021 agaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    52081 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    52141 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    52201 ggacgatggg gcgctgtaat gattgcggag agtgcgtgcc gcagtgtccg catcaggcgt
    52261 tgcagattgt cgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct
    52321 gcctgaagat gtgtccgcaa catgccacgc caatggcgca gtccatgagc gtggatgagg
    52381 tgcttagcca tatccgcaaa gcagtgctgt ttatcgaagg gataacggta agcggcggcg
    52441 aagccacgac ccagctaccg tttgtggtgg cgctgtttac tgctatcaaa aacgatccgc
    52501 aactgcgcca cctcacctgt ctggtggaca gtaatggcat gttgagcgaa accggctggg
    52561 aaaaattgct tccggtgtgc gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat
    52621 gtcatcagca attaaccgga cgcgataatc agcagatcaa gcgcagcatc tgtttgctgg
    52681 cagagcgcgg taagctggcg gaactgcgtt tgctggtaat ccctggccag gtggattatt
    52741 tgcaacacat cgaagaactg gcggcgttaa tcaagggact tggcgatgtt ccggtacgcc
    52801 tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctggccg agcgccacgc
    52861 cggaggatgt ggaacaactg gctgatgcgt tatgggagcg cggggtgagc cggttaatat
    52921 ttccggcgct atatttgtga catgccggat acggcctacg gatcggcaca gacctgtagg
    52981 catgataaga tgcgtcaagc gtcgcatcag gcattgtgcg ccagctgccg gatgcggcgt
    53041 gaacgcctta tccggcctac ggttcggcac agacttgtag gcatgataag acgcgtcaag
    53101 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    53161 cggcacgtta aacaacgtat acgtattatt tagcaacact tcggcaatct catcttctgg
    53221 ttccgggcgt aactcgcaaa gtacgtcaaa cacccgcgca gcctgttccg ggcggttagg
    53281 ctgcccctga aagccgttga gcggcatatc cggcgcgtcg gtttccagca acaacgatgc
    53341 cagcggtaat tttgcgatga catcgcgggt tttactggcg cgtggatagg tgatagtacc
    53401 gcctacgccg attttgtagc ccagcagtac aaacctttcg gcctgttgca ggctgccgga
    53461 aaaaccgtga accacgccag tgcgtggcaa atcgtggcgt ttaagatgca tcgccagttt
    53521 gtcgtgtgtg cgccgtgaat gcaggatcac cggcagatcg tagcgtttcg ccagtttcag
    53581 ttgttcgtcg agtaaccact gctgcctctc aaattgcgga tcgtcgccga agagatccag
    53641 accgatctcc cccaccgcca ccaccttcgc cggacgcctt tccagcgcct gctgtagctg
    53701 atcaagagac acatcgctat gttttttcaa cataccagga tgcaagccca atgcggcata
    53761 cagcggttga tatttttccg ctaacgcctg cacgcgggca aaattttccg cctctgtcgc
    53821 cggaacaatg atcttgccta cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc
    53881 accactaaaa ggcgggaaat caaaatggca gtgggtgtcg ataaaacggc aaatcaagcc
    53941 agatcctcat tgttaaatgt cgggtcgttc gcctgcggag cgtcgatcac ttcggcaacc
    54001 agcgtatcgt tggcgactgg cgcaggcgga atgacaatcg attccggcgt caccaccggc
    54061 accagatggc gggtaagcgg cgctttttca gtcaataact taccgaccgt tgccagaaaa
    54121 taacgaccgc ataaacgccc aagtttatag tcttcacgca gcgccggaat ccgactgcca
    54181 agcgcgatac tatgcaacgg cttcggcgga taaatttcga atatccgcag ctggcccggt
    54241 ggtttctcaa taaattgctg aatgtcacga tagctggttt catgatgctg caccagattg
    54301 accagcggct gcaggctact gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc
    54361 gtgtagtaca tttgcgacgg cacggtgcgg atgacgacca aggtttttgc tccctgcctt
    54421 gccgcctctt taaccggaat cgcatcactg atcccgccat ccaggtagtt aatgccttcc
    54481 agcgacactc cgctacgata aaagccaggt atcgcactgg aggcgcgaat cacatccagc
    54541 cagttttgtt tggttggtaa aaagtaattc ggcgcatagt catcctgacg acaggcgcac
    54601 atataaaacg atttgccgct gtcaaacaac cgcgcggcgg tatccatttg caacggcatc
    54661 tggcttgcag tggcctccac cagccagtcg agatcgataa gatttcctcc acggacaaag
    54721 cgcaatggat cgaaaaattc gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa
    54781 ccgggctgat tgcagatata cgccgagagg ttctgcgccc cggcagatgt gccgagataa
    54841 agatcgaaag gattaaactg cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt
    54901 ccacgctgtc cgccgccttc gcacaccaga gctattcgtc caggctggaa cggccttagc
    54961 gacaacggcg caatattacc aagcgttaca ggtattcgct gccccacctc tgccttcctg
    55021 tttttgttgt gaaagaatga caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc
    55081 cagtccgctg gactggcttt ggataaaatt ttcttaatgg tctgaaatct agggtcgttt
    55141 tcggcccatg aacaaactca ccaggaacag aataatcccg acgacaaaga caattttagc
    55201 tgcgcctgca gcggtaccgg ccagaccacc aaacccaagt gcggcggcga ttaacgcgat
    55261 aaccagaaat atgatgcccc aacgaaacat aagcgtctcc tttaccatag ttaatgtcac
    55321 cgcttgatat gagcgtgaaa atcactcaat gcgatatttt tagtgtggtg cacatcgcgc
    55381 ctcccgacaa agttcgggag gacgaattac gacgaattac ttagttttca gatcattttt
    55441 aacgcttttc acaccatcta ccgctttggc gatactttca gcacggtcgc tctgtgcctg
    55501 ggaatcgacg gtaccggaga gctgaaccac gccgtcggtg gtttcaactt tcacatgacg
    55561 ggaagggacg atatcgtccg ccagcagttt ggctttgatt tcactggtgg tggcggtgtc
    55621 acccgcgtag cccttcaccg agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac
    55681 agaggtcacc ccttcaacgc ctttcgccac tttcactgcc tcttcggcct gagcctggct
    55741 ttcaacgaaa ccgctcaggg tcacgacttt ttgatcggtt ttgacagaga tatcggtgct
    55801 cttgatgttg tcatgatcca ccagcgccgc cttcactttc gcggtgatgg cgctgtcatc
    55861 catgaaatta ccgactttat tcatagagct atcgactttt tgccctgcgc tttcattggt
    55921 agtctgtgcg ttgttttccg cgtaggcaga gccggtcgcg acggcagagg tcaacattac
    55981 agccagcaga gttttcgaaa tcttcagtct tgtcatagtc atcgatttat tcctgtatgt
    56041 ttgctcgtaa tttgagcctg gcaacacgag gttgcattgc tgaataggga gagacttcac
    56101 cctctacaga agtcaatggc cgccatcaca aaagcgatgg gtgatgaata acgaccatta
    56161 cagcctctga atcagttatt aatatcggta gaatgacaat cgacggcttt agatactgat
    56221 atctacgcat tgaacggtat ttaacgccgt cagaaatgtc attactttgt taaatataga
    56281 tcacaatttt gaaaccgctc gggatatcag cgaaaacata agcaaaagtg aatgttttaa
    56341 gaacattccg taagcggcta ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc
    56401 cctggaggca agaattaatg ctcgcgggtc tggtggaact gaacgtctgg ataacgttcc
    56461 tgcgccaggc gcaggttgac catgctggta gcgatgtaag cgaggttatc gccgccatca
    56521 agcgccagtt ggctttcgtt cttacgcttg aactcttcga atttcttcgc gtccgcacat
    56581 tctacccagc gggcagtggc aacgttgact gactcataca ctgcttcaac gttgtattcg
    56641 ctcttcagac gcgctaccac cacatcaaac tgcagcacac caaccgcacc aacgatcaga
    56701 tcgttgttgg agatcggacg gaacacctgc accgcgccct cttcggaaag ctgtaccagc
    56761 cctttaagca gctgtttttg cttcagcgga tctttcaggc ggatacgacg gaacagttct
    56821 ggcgcgaagt tcggaatacc ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg
    56881 atctgaatgg tgccgtggtt gtgcagacca aggatatcgc caggatacgc ttcttcaacg
    56941 tgcgaacggt cacccgccat aaaggtcagc gcgtcggaga tcaccacatc tttcgcagta
    57001 cgcacctggc gcagcttcat gcctttttca tatttaccgg acaccacacg cataaacgcc
    57061 acgcggtcgc ggtgtttcgg gtccatgttg gcctgaattt taaatacgaa gccggtaaat
    57121 ttgtcttcgc tcgcctctac ggtacgggta tcagtctgac gcggcatcgg cgcaggagcc
    57181 cactccacca ggccatccag catatgatca acgccgaagt tacccagcgc agtaccgaag
    57241 aataccggag tgatttcgcc cgcaaggaac agctctttgt cgaactcgtt agacgcgcct
    57301 ttcaccagct ccagttcgtc acgtagctgc tgtgccagat cttcaccaac cgcagcgtcg
    57361 agatccgggt tattcagccc tttaacaatg cggacttcct gaatcgtgtg gcctttaccg
    57421 ctctgataga gataggtttc atctttataa aggtggtaaa cgcctttaaa cagcttgccg
    57481 cagccaatcg gccaggtgat cggtgcgcag ccgattttca gctcgttctc aacttcatcg
    57541 agcagctcca tcgggtcgcg gatatcacgg tcaagtttgt tcataaaggt gaggatcggc
    57601 gtgtcgcgca gacgggtaac ttccatcagc ttacgggtac gatcttcaac accttttgcg
    57661 gcgtcgataa ccatcaggca gcagtccacc gccgtcaggg tacgataggt atcttccgag
    57721 aagtcttcgt gccccggggt gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc
    57781 atcacagacg tagtaatgga gatcccacgc tgcttttcca tctccatcca gtccgactta
    57841 gcgtgctggt tggaaccacg gccttttact gtaccggcgc tctgaatggc ctgtccgaac
    57901 agcagcacct tctcggtgat ggtagtctta ccggcgtccg ggtgagaaat aatggcaaaa
    57961 gtgcggcgct tcgccacctc ttgcaaataa ggagacaacg tcataattct ttcttcttga
    58021 gtaagcggca tcgcgccgcg cttgttgaaa acgaaaaatt gcggctattt tacccatcaa
    58081 cgggggggag gcaatcagtg tttacacagg agctgctcca gttcgtgcaa cgaagaaacg
    58141 gtccaggtgg gcgcgatgcc ttctggttgc tcgcgattgt gcgcattcag ccagcaggtc
    58201 gcaagcccgg cgttgatgcc accgagaata tcggactcag ctgtgtcgcc aaccatcagc
    58261 acacgtgaac ggtcaggatt gcctgcctgt tccagcgcat aatcgaaaat tttcttattc
    58321 ggtttggcaa cgccaacttc ttcggaaatc accagcaaat cgaaataatc acgcaggccc
    58381 gtgcgttcca gacgcacttg ctgcaaggcg ctaaagccgt tggtgatgat gccaatttta
    58441 gcgttgccac gaatggcgtt aagcagagaa accgcgcccg gcagtggcgt acagatttcc
    58501 gccatcgcat taataaaggc ttcgttgagt ttacccggct cgacgttcag ccgttcggcc
    58561 cagctctcaa accgcccgtg ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc
    58621 acccacagtg gcttgttaac ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg
    58681 ctgtaatcaa gaaacatccg ctgcaagccg gtgaatgagt caaaggtaaa cagcgtttca
    58741 tcggcatcaa agaaaatcca gtcccacttc attattccac cttgtcttac atactgattg
    58801 gcaacgccat gatgatggcg tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa
    58861 ttgtcgcctc gttaaagcct aaactttcgt acagggcaat ggcggcagcg tttgaagcac
    58921 ggacttccag ccatagcgtc gccacgccgc gtttttccag ttcgtcgatc agatgttcca
    58981 gcagcgccct tcccaatccc tgacgctgat agtcaggatc gaccgcaata ttgaacaatg
    59041 tagcttcatc cagcaccact tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta
    59101 actgaaagtt gagataacgc tcgccctggt tgctggcaaa ggttttttca ctccacggaa
    59161 aggcgtgggc gcgttgttca atgtggtaag ccgctggtaa atcagtcgtt tcgagggaag
    59221 aaatcgtgtt catatgtgca aatttgttgc cataacgcgg cgcgtgccgt tgggtttgcc
    59281 cgtaattcgg tgagcgccgg tgatgccacc tgagcgcctt ccagtgatag cggttcgtca
    59341 gtacccaacc gccagctgtt gcagcgactg ccttgcggca gcatcgcgat tttttctggc
    59401 gtcagttgca gcacttgatc ggggctgacg gttaacgcgc gcagaacatc gctcactata
    59461 ggatcggtca gggcgggaag atcgtttgcc accatcacca gacggacgtg tgccgggatc
    59521 gcaatggcaa tctccccctg caacgcgcca gggcgacgca gcgaccactg ggtaatgccc
    59581 agttgctgta actgccagtc tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg
    59641 cgcaaatata gcaaattcgt cgataccgcg ccaacatatg gctataatcg ccgccagtat
    59701 taattgagga gcattccatg tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca
    59761 gtgatgattt cgaacaaagc cgtattcttt ttgccggaga cttacaggat gacctgcccg
    59821 cgcgtttaga taccgcggcc agccgtgctc atacccagca attccaccac tggcaggtgt
    59881 taagccgcca gatgggggat aacgcccgtt ttagtctggt cgccacggcg gatgatgtcg
    59941 cagattgcga tacgctgatt tactactggc cgaagaacaa accggaagcc cagttccagt
    60001 tgatgaattt actttctctg ctgccagtgg gcacggatat ttttgtcgtt ggcgagaacc
    60061 gcagcggcgt gcgcagcgcc gagcagatgc tgtcagatta tgcgccgttg aataaaatgg
    60121 acagcgctcg tcgctgtggc ctctattttg gtcgtctgga aaaacagcca gtatttgatg
    60181 ccgataaatt ctggggcgaa tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg
    60241 tgtttagccg cgacggtctg gatgtcggta gccagttgct gctctcgacg ttaaccccgc
    60301 acacgaaagg taaagtgctg gatgtcggct gtggcgcggg cgtactttcg gttgcctttg
    60361 cgcgccattc gccgaaaatt cgtctcactc tgtgcgatgt ctctgcacca gcggtagaag
    60421 ccagccgcgc aacacttgcg gccaacggtg ttgaaggtga agtctttgcc agtaacgtct
    60481 tttccgaggt gaaaggtcgt tttgatatga tcatctccaa cccgccgttc cacgatggga
    60541 tgcaaaccag cctggatgcc gcgcaaacgc tgattcgcgg cgcggtgcgt catcttaata
    60601 gcggcggcga gctgcgaatt gtagcgaacg ccttcctgcc ttatccggac gtgctggatg
    60661 agacatttgg cttccacgaa gtgatcgcgc aaaccgggcg cttcaaggtg tatcgcgcca
    60721 ttatgacccg ccaggcgaag aaaggttgag tatctgatat atgtcgccgc ataccgtgtc
    60781 ggatgcggcg tacacgcctt atctgaccga caaacgaatt atccacaatc cgctgatgga
    60841 catttttcca gcaattacac ctctgtcgat aattaactat tgacgaaaag ctgaaaacca
    60901 ctagaatgcg cctccgtggt agcaattctt tttaagaatt gacggtatgc gaaggtggcg
    60961 gaattggtag acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg ggttcaagtc
    61021 ccccccctcg caccaattat ctttacttcc tttcttgttt cttccctgtt ttatcatccc
    61081 tgcttcaaat tcattatcat cagcatgatg ccagcaataa gcgccaggca cgatggcagc
    61141 aaaacgtaat gccgcaactg tttgtgatac agcatcgccg gcgtcaatac taatcccatc
    61201 agaatgataa tccgccattg atccggcgat aaatccgcat acatcagccc tgcaatcagc
    61261 aatcccggca gcaacactcc ccagccactg cccagcgtac gttgcaacat ggtttcatct
    61321 ctttcattga taatgataac caatatcata tgataatttt tatcatttgc aagccagaga
    61381 aatcccttgc tatcgggtaa acctatcgct aggattagca atcattatca tttagattac
    61441 tatcacgatt atggcctatc gttccgcacc gctctatgaa gatgttatct ggcgaacgca
    61501 tctccagccg caggatgctg ggcttgcaca agccgtacgg gcgacgatcg ccgaacatcg
    61561 tgaacatttg ctggagttta tccgtctgga tgaacccgca ccgctccgtg ccatgacgct
    61621 ggcgcaatgg tcatcaccga atacgctaag ttctcaactg gcggtctatt ccgatcatat
    61681 ctaccgcaac caacccttga tgatccgcga gaacaaaccg ctgatctcac tatgggcaca
    61741 atggtatatc ggcctgatgg tgccaccatt aatgttggcg ctgctgacgc aggaaaaagc
    61801 attagatgtg tcgccggaac atttccacgt tgagtttcac gaaaccggac gcgccgcctg
    61861 tttctgggtc gatgtgtgtg aagataaaaa cgcaacacta cattcgccgc agcagcgaat
    61921 ggaaacgtta atcagccagg cgcttgttcc ggtagtgcag gcattagaag cgacgggaga
    61981 gatcaacggc aaacttatct ggagcaatac cggttatttg atcaactggt atctgactga
    62041 gatgaaacag ctgcttggcg aggcgacggt tgaatcgctg cgccatgccc tcttttttga
    62101 gaaaatgctc acgaacggtg aagataatcc actctggcgt accgtggtac tgcgcgacgg
    62161 cctgctggtg cgccgcactt gctgccagcg ttatcgctta ccggatgtgc agcaatgtgg
    62221 cgattgtacg ctgaaatgaa gcactgccgg ggaagtaaac ccggcatcat gtggattaat
    62281 agggatgcaa cacattactt gcttcgcaat gccgcataca taacagaata tctgcggcct
    62341 ccaacaaccc tgcatcagaa ctgacgccca gtttcgacat cacattaaat ttatgtgcac
    62401 ggatcgtttt gatattgcgt ttaagctgct cggcaatttg tgtcattgag tagccacgcg
    62461 acataaagcg caatatttct ctctccgtcg ggcttagcgt tcggctttga ttaatgtacc
    62521 actgattgtt cagtcggtcg gtcgcctgac gtacaccatt taatgacaaa aagagttcct
    62581 gatgaaaaat ctccagcgtt gacgctttac ttaatacacc gtccaacggt gatggcgaca
    62641 atgaaccaat cagccgagct tcaatatcat catccgcaat aactaaacgc cgggtacgcg
    62701 gaaacttaat cgccagttca gtcaggcaag ataatccctc tctgcgctca cttctcatgg
    62761 cagaaaaaga aaaaatgacc gccgcaaacg agatccgcga catggcatcc tgaaatgact
    62821 cctgactggt ataggtatgg agcttataag ggttacccgc aaactttctg aataaactct
    62881 caataccgat actgctcatg acgcattttt ctatgagtgc gacatttctc ttcttaattc
    62941 ggctgtgttc cattctgcgg catctccata aacagaaggc aagttaagtt ctcttgcacc
    63001 ctgcgcgcta tttatccatg catacatttc agcgttgctg tgaattgcca gtcgacgcat
    63061 cgcactgttt ttttgggcgc tgatagtttt attacttttc ttaagcaatg aagctatctg
    63121 gttgatgccc catcctttac ctaatagccg cagtaccttg cgctctgaaa gagtgagaat
    63181 gacggagcga tagctaaaat catgagtttc agtcattgcc ggagaggtca gcatggcgct
    63241 gatacgctct gcgtgcgtat tgccggaacg tacggtcttg accagattct caatgggttc
    63301 aacatcagac aataacgtcg ccgtagggca cataagcagt tctactgcct gggaatacca
    63361 ggagcgtgac accatgaaaa cccagtgaat ttcccgatat tgtgagatta aagaataata
    63421 atgttcacaa acagaacgtg gatcttcact ttcaccggct aaatcagcaa tgactaatcc
    63481 ggaacgacgt aattgtaata aggtaaggtc ctccgcagag gcgctggata ttatttcata
    63541 ttcagggaag tgagtcctca tgacctcttt taaccctgct tgcataacag gtattttact
    63601 gataacaatt ccatttttgc agcatcctgg caacatattt cctccacatc cactgtctaa
    63661 aaatcatgat ggtttattca ggatattcat tccttaaatg tttccagcaa attaatagat
    63721 taagcgaata aattatcctt agcattcact ttcatatcag catactcctg ctgcacaaca
    63781 actccactgc atgtgtcaga aggttagcat caaatgataa cctggtgaaa tagcctggag
    63841 acatttaaaa tatctacatt aagttgttag aaatacttaa tgaaaaacag aattctcatt
    63901 catttaattt ataattaact gatatccctt c
//
LOCUS       DAZGTJ010000017        62859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000017 DAZGTJ010000000
VERSION     DAZGTJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.017"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..305
                     /locus_tag="ACHK8E_001405"
     CDS             <1..305
                     /locus_tag="ACHK8E_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0343695.1"
                     /translation="VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLP
                     NPNDKRGVLVKLTTSGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            326..709
                     /gene="marA"
                     /locus_tag="ACHK8E_001406"
     CDS             326..709
                     /gene="marA"
                     /locus_tag="ACHK8E_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP0343696.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            741..959
                     /gene="marB"
                     /locus_tag="ACHK8E_001407"
     CDS             741..959
                     /gene="marB"
                     /locus_tag="ACHK8E_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP0343697.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(990..1889)
                     /gene="eamA"
                     /locus_tag="ACHK8E_001408"
     CDS             complement(990..1889)
                     /gene="eamA"
                     /locus_tag="ACHK8E_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP0343698.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            2084..3271
                     /gene="ydeE"
                     /locus_tag="ACHK8E_001409"
     CDS             2084..3271
                     /gene="ydeE"
                     /locus_tag="ACHK8E_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP0343699.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3398..3493
                     /gene="mgtS"
                     /locus_tag="ACHK8E_001410"
     CDS             3398..3493
                     /gene="mgtS"
                     /locus_tag="ACHK8E_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP0343700.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3493..3597
                     /gene="mgtT"
                     /locus_tag="ACHK8E_001411"
     CDS             3493..3597
                     /gene="mgtT"
                     /locus_tag="ACHK8E_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP0343701.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(3712..4602)
                     /gene="dgcZ"
                     /locus_tag="ACHK8E_001412"
     CDS             complement(3712..4602)
                     /gene="dgcZ"
                     /locus_tag="ACHK8E_001412"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP0343702.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4857..5249)
                     /gene="ydeI"
                     /locus_tag="ACHK8E_001413"
     CDS             complement(4857..5249)
                     /gene="ydeI"
                     /locus_tag="ACHK8E_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP0343703.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            5489..5569
                     /locus_tag="ACHK8E_001414"
                     /pseudo
     CDS             5489..5569
                     /locus_tag="ACHK8E_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5614..7659)
                     /gene="dcp"
                     /locus_tag="ACHK8E_001415"
     CDS             complement(5614..7659)
                     /gene="dcp"
                     /locus_tag="ACHK8E_001415"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP0343704.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            7796..8542
                     /gene="ydfG"
                     /locus_tag="ACHK8E_001416"
     CDS             7796..8542
                     /gene="ydfG"
                     /locus_tag="ACHK8E_001416"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP0343705.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            8631..9317
                     /gene="rspR"
                     /locus_tag="ACHK8E_001417"
                     /gene_synonym="ydfH"
     CDS             8631..9317
                     /gene="rspR"
                     /locus_tag="ACHK8E_001417"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP0343706.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            9494..9697
                     /gene="ydfZ"
                     /locus_tag="ACHK8E_001418"
     CDS             9494..9697
                     /gene="ydfZ"
                     /locus_tag="ACHK8E_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP0343707.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(9733..11193)
                     /gene="ydfI"
                     /locus_tag="ACHK8E_001419"
     CDS             complement(9733..11193)
                     /gene="ydfI"
                     /locus_tag="ACHK8E_001419"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0343708.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(11283..12650)
                     /gene="ydfJ"
                     /locus_tag="ACHK8E_001420"
     CDS             complement(11283..12650)
                     /gene="ydfJ"
                     /locus_tag="ACHK8E_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFP0343709.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            complement(12708..13727)
                     /gene="rspB"
                     /locus_tag="ACHK8E_001421"
     CDS             complement(12708..13727)
                     /gene="rspB"
                     /locus_tag="ACHK8E_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP0343710.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(13739..14953)
                     /gene="rspA"
                     /locus_tag="ACHK8E_001422"
     CDS             complement(13739..14953)
                     /gene="rspA"
                     /locus_tag="ACHK8E_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP0343711.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(15159..15485)
                     /gene="ynfA"
                     /locus_tag="ACHK8E_001423"
     CDS             complement(15159..15485)
                     /gene="ynfA"
                     /locus_tag="ACHK8E_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP0343712.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            15620..15961
                     /gene="ynfB"
                     /locus_tag="ACHK8E_001424"
     CDS             15620..15961
                     /gene="ynfB"
                     /locus_tag="ACHK8E_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP0343713.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            15996..16556
                     /gene="speG"
                     /locus_tag="ACHK8E_001425"
     CDS             15996..16556
                     /gene="speG"
                     /locus_tag="ACHK8E_001425"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP0343714.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(16559..17269)
                     /gene="ynfC"
                     /locus_tag="ACHK8E_001426"
     CDS             complement(16559..17269)
                     /gene="ynfC"
                     /locus_tag="ACHK8E_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP0343715.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            17377..17682
                     /gene="ynfD"
                     /locus_tag="ACHK8E_001427"
     CDS             17377..17682
                     /gene="ynfD"
                     /locus_tag="ACHK8E_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP0343716.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            17881..20307
                     /gene="ynfE"
                     /locus_tag="ACHK8E_001428"
     CDS             17881..20307
                     /gene="ynfE"
                     /locus_tag="ACHK8E_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP0343717.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            20368..22791
                     /gene="ynfF"
                     /locus_tag="ACHK8E_001429"
     CDS             20368..22791
                     /gene="ynfF"
                     /locus_tag="ACHK8E_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP0343718.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            22802..23419
                     /gene="ynfG"
                     /locus_tag="ACHK8E_001430"
     CDS             22802..23419
                     /gene="ynfG"
                     /locus_tag="ACHK8E_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP0343719.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            23421..24275
                     /gene="ynfH"
                     /locus_tag="ACHK8E_001431"
     CDS             23421..24275
                     /gene="ynfH"
                     /locus_tag="ACHK8E_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP0343720.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            24318..24932
                     /gene="dmsD"
                     /locus_tag="ACHK8E_001432"
     CDS             24318..24932
                     /gene="dmsD"
                     /locus_tag="ACHK8E_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP0343721.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            25091..26383
                     /gene="clcB"
                     /locus_tag="ACHK8E_001433"
     CDS             25091..26383
                     /gene="clcB"
                     /locus_tag="ACHK8E_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP0343722.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(26336..27031)
                     /gene="bioD"
                     /locus_tag="ACHK8E_001434"
     CDS             complement(26336..27031)
                     /gene="bioD"
                     /locus_tag="ACHK8E_001434"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0343723.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(27156..28376)
                     /gene="mlc"
                     /locus_tag="ACHK8E_001435"
                     /gene_synonym="dgsA"
     CDS             complement(27156..28376)
                     /gene="mlc"
                     /locus_tag="ACHK8E_001435"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP0343724.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(28511..29404)
                     /gene="ynfL"
                     /locus_tag="ACHK8E_001436"
     CDS             complement(28511..29404)
                     /gene="ynfL"
                     /locus_tag="ACHK8E_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0343725.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMMDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            29511..30764
                     /gene="ynfM"
                     /locus_tag="ACHK8E_001437"
     CDS             29511..30764
                     /gene="ynfM"
                     /locus_tag="ACHK8E_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343726.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(30814..30933)
                     /locus_tag="ACHK8E_001438"
     CDS             complement(30814..30933)
                     /locus_tag="ACHK8E_001438"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP0343727.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            31189..31497
                     /gene="asr"
                     /locus_tag="ACHK8E_001439"
     CDS             31189..31497
                     /gene="asr"
                     /locus_tag="ACHK8E_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP0343728.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            31590..31673
                     /locus_tag="ACHK8E_001440"
     CDS             31590..31673
                     /locus_tag="ACHK8E_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343729.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            31773..32594
                     /gene="ydgD"
                     /locus_tag="ACHK8E_001441"
     CDS             31773..32594
                     /gene="ydgD"
                     /locus_tag="ACHK8E_001441"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP0343730.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(32633..32962)
                     /gene="mdtI"
                     /locus_tag="ACHK8E_001442"
     CDS             complement(32633..32962)
                     /gene="mdtI"
                     /locus_tag="ACHK8E_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP0343731.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(32949..33314)
                     /gene="mdtJ"
                     /locus_tag="ACHK8E_001443"
     CDS             complement(32949..33314)
                     /gene="mdtJ"
                     /locus_tag="ACHK8E_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP0343732.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            33725..34759
                     /gene="tqsA"
                     /locus_tag="ACHK8E_001444"
     CDS             33725..34759
                     /gene="tqsA"
                     /locus_tag="ACHK8E_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP0343733.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(34784..36172)
                     /gene="pntB"
                     /locus_tag="ACHK8E_001445"
     CDS             complement(34784..36172)
                     /gene="pntB"
                     /locus_tag="ACHK8E_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP0343734.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(36183..37715)
                     /gene="pntA"
                     /locus_tag="ACHK8E_001446"
     CDS             complement(36183..37715)
                     /gene="pntA"
                     /locus_tag="ACHK8E_001446"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP0343735.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            38239..39183
                     /gene="ydgH"
                     /locus_tag="ACHK8E_001447"
     CDS             38239..39183
                     /gene="ydgH"
                     /locus_tag="ACHK8E_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP0343736.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            39369..40751
                     /gene="ydgI"
                     /locus_tag="ACHK8E_001448"
     CDS             39369..40751
                     /gene="ydgI"
                     /locus_tag="ACHK8E_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0343737.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            40788..41510
                     /gene="folM"
                     /locus_tag="ACHK8E_001449"
     CDS             40788..41510
                     /gene="folM"
                     /locus_tag="ACHK8E_001449"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP0343738.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(41507..41842)
                     /gene="ydgC"
                     /locus_tag="ACHK8E_001450"
     CDS             complement(41507..41842)
                     /gene="ydgC"
                     /locus_tag="ACHK8E_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP0343739.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            41971..42690
                     /gene="rstA"
                     /locus_tag="ACHK8E_001451"
     CDS             41971..42690
                     /gene="rstA"
                     /locus_tag="ACHK8E_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP0343740.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            42694..43995
                     /gene="rstB"
                     /locus_tag="ACHK8E_001452"
     CDS             42694..43995
                     /gene="rstB"
                     /locus_tag="ACHK8E_001452"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP0343741.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            44071..45000
                     /gene="tus"
                     /locus_tag="ACHK8E_001453"
     CDS             44071..45000
                     /gene="tus"
                     /locus_tag="ACHK8E_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP0343742.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(44997..46400)
                     /gene="fumC"
                     /locus_tag="ACHK8E_001454"
     CDS             complement(44997..46400)
                     /gene="fumC"
                     /locus_tag="ACHK8E_001454"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP0343743.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(46543..48189)
                     /gene="fumB"
                     /locus_tag="ACHK8E_001455"
     CDS             complement(46543..48189)
                     /gene="fumB"
                     /locus_tag="ACHK8E_001455"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0343744.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            48388..49563
                     /gene="manA"
                     /locus_tag="ACHK8E_001456"
     CDS             48388..49563
                     /gene="manA"
                     /locus_tag="ACHK8E_001456"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP0343745.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            49664..51172
                     /gene="ydgA"
                     /locus_tag="ACHK8E_001457"
     CDS             49664..51172
                     /gene="ydgA"
                     /locus_tag="ACHK8E_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0343746.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(51217..52482)
                     /gene="uidC"
                     /locus_tag="ACHK8E_001458"
     CDS             complement(51217..52482)
                     /gene="uidC"
                     /locus_tag="ACHK8E_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP0343747.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(52521..53894)
                     /gene="uidB"
                     /locus_tag="ACHK8E_001459"
     CDS             complement(52521..53894)
                     /gene="uidB"
                     /locus_tag="ACHK8E_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP0343748.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            complement(53891..55702)
                     /gene="uidA"
                     /locus_tag="ACHK8E_001460"
     CDS             complement(53891..55702)
                     /gene="uidA"
                     /locus_tag="ACHK8E_001460"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP0343749.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(56089..56679)
                     /gene="uidR"
                     /locus_tag="ACHK8E_001461"
     CDS             complement(56089..56679)
                     /gene="uidR"
                     /locus_tag="ACHK8E_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP0343750.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            complement(56900..57667)
                     /gene="hdhA"
                     /locus_tag="ACHK8E_001462"
     CDS             complement(56900..57667)
                     /gene="hdhA"
                     /locus_tag="ACHK8E_001462"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP0343751.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(57779..58807)
                     /gene="malI"
                     /locus_tag="ACHK8E_001463"
     CDS             complement(57779..58807)
                     /gene="malI"
                     /locus_tag="ACHK8E_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP0343752.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            58982..60574
                     /gene="malX"
                     /locus_tag="ACHK8E_001464"
     CDS             58982..60574
                     /gene="malX"
                     /locus_tag="ACHK8E_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP0343753.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            60584..61756
                     /gene="malY"
                     /locus_tag="ACHK8E_001465"
     CDS             60584..61756
                     /gene="malY"
                     /locus_tag="ACHK8E_001465"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP0343754.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            61860..>62859
                     /gene="add"
                     /locus_tag="ACHK8E_001466"
     CDS             61860..>62859
                     /gene="add"
                     /locus_tag="ACHK8E_001466"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP0343755.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
BASE COUNT        15848 a        16155 c        15042 g        15814 t
ORIGIN      
        1 aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaggtat
       61 tgtcggtcga cctgggggcg ctgacccgta tgctggatcg cctggtctgt aaaggctggg
      121 tggaaaggtt gccgaacccg aatgacaagc gcggcgtact ggtaaaactt accaccagcg
      181 gcgcggcaat atgtgaacaa tgccatcaat tagttggcca ggacctgcat caagaattaa
      241 caaaaaacct gacggcggac gaagtggcaa cacttgagca tttgcttaag aaagtcctgc
      301 cgtaaacaaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
      361 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
      421 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
      481 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
      541 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
      601 gacccgaacc ttcaaaaatt actttgatgt tccgccacat aaataccgga tgaccaatat
      661 gcagggtgaa tcgcgctttt tacatccatt aaatcattac aacaactagt tgataacgtg
      721 acaacgtcac tgaggcaatc atgaaaccac ttttatccgc aatagcaact gcgcttattc
      781 tcttttctgc gcagggcgtt gcggaacaaa ccacgcagcc ggttgttact tcctgtggca
      841 atgtcgtggt tgttcccaca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
      901 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
      961 ttgttctggc cccgacatat cggggcttat taacttccca cttttaccgc tttacgccac
     1021 cgcaagccaa atacattgat atacagcccg gtcataatga gcaccgcacc gaaaaattgt
     1081 agacccgtta agcgttcatc caataatagt gccgcacttg ccagtcctac tacgggcacc
     1141 agtaacgata acggtgcaac ccgccaggtt tcatagcgtc ccagtaacgt cccccagatc
     1201 ccataaccaa caattgtcgc cacaaacgcc agatacatca gagacaagat ggtggtcata
     1261 tcgatagtaa ccagactgtg aatcatggtt gcggaaccat cgagaatcag cgaggcaaca
     1321 aagaagggaa tgattgggat taatgcgctc cagattacca gcgacatcac cgccgggcgc
     1381 gttgagtgcg acatgatctt tttattgaag atgttgccac acgcccaact aaatgctgcc
     1441 gccagggtca acataaagcc gagcatcgcc acatgctgac cgttcagact atcttcgatt
     1501 aacaccagta cgccaaaaat cgctaaggcg atccccgcca attgtttgcc atgcagtcgc
     1561 tccccgaaag taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg taacaccagc
     1621 gaagccagtc cagcaggcat accaaagtta atggcgcaaa acagaaaggc aaactgcgca
     1681 aaactgatgg ttaatccata ccccagcagc aaattcagtg gtactttcgg tcgagcgaca
     1741 aaaaagatgg ccggaaaagc gaccagcata aagcgcaaac cggccagcat cagcggtggc
     1801 atgttatgaa gccccacttt gatgaccaca aaattcagcc cccatacgac cactaccagt
     1861 agcgccaaca ccccatcttt tcgcgacatt ataccgcctc tgaatttcat cttttgtaag
     1921 caatcaactt agctgaattt acttttcttt aacagtcgat tcgttagtca ccgtttacga
     1981 cggcattaat gcgcaaataa gtcgctatac ttcggatttt tgccatgcta tttctttaca
     2041 tctctaaaac aaaacataac gaaacgcact gccggacaga caaatgaact tatccctacg
     2101 acgctctacc agcgcccttc ttgcctcgtc gttgttatta accattggac gcggcgctac
     2161 gctgccgttt atgaccattt acttgagtcg ccagtacaac ctgagtgtcg atctaatcgg
     2221 ttatgcgatg acaattgcgc tcactattgg cgtcgttttt agcctcggtt ttggtatcct
     2281 ggcggataag ttcgacaaga aacgctatat gttactggca attaccgcct tcgccagcgg
     2341 ttttattgcc attcctttag tgaataacgt gacgctggtg gtgctctttt ttgccctcat
     2401 taactgcgcc tattccgttt ttgctaccgt gttgaaagcc tggtttgccg acaatctttc
     2461 gtccaccagc aaaacgaaaa tcttctcaat caactacacc atgctcaaca ttggctggac
     2521 catcggtccg ccgctcggca cgttgttggt gatgcagagc atcaatctgc ccttttggct
     2581 ggcagctatc tgttccgcgt ttcccatgct tttcattcaa atttgggtaa agcgcaacga
     2641 gaaaatcatc gccacggaaa caggcagtgc ctggtcgccg aaagttttat tacaagataa
     2701 agcactgttg tggtttacct gctctggttt tctggcttcc tttgttagcg gcgcatttgc
     2761 ctcgtgcatt tcacaatatg tgatggtgat tgctgatggt gattttgccg aaaaggtggt
     2821 tgcggttgtt cttccggtta atgctgccat ggtagttacg ttgcaatatt ccgtgggccg
     2881 ccgacttaac ccggctaaca tccgcgcgct gatgacagca ggcaccctct gtttcgtcat
     2941 tgggctggtc ggttttattt tttccggcaa cagcctgcta ttgtggggta tgtcagctgc
     3001 ggtatttact gtcggtgaaa tcatttatgc gccgggcgag tatatgttga ttgaccatat
     3061 tgcgccgcca ggaatgaaag ccagctattt ttccgctcag tctttaggct ggcttggtgc
     3121 ggcgattaac ccattagtga gtggcatagt gcttaccagt ctgccgcctt tctcgctgtt
     3181 tatcatctta gcgttggtga tcattgctgc gtgggtgctg atgttaaaag gaattcgtgc
     3241 aagaccgtgg gggcagcccg cgctttgttg atttaagtcg aacacaataa aaatttaatt
     3301 cagccttcgt ttaggttacc tctgctaata tctttctcat tgagatgaaa attaaggtaa
     3361 gcgaggaaac acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt
     3421 tatggccgta ctgggaataa ttttattttc tggttttctg gccgcgtatt tcagccacaa
     3481 atgggatgac taatgaacgg agataatccc tcacctaacc ggccccttgt tacaactgtg
     3541 tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg
     3601 cttgcatgga tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa
     3661 cggatcgttg acacagtagc atcagttttc tcaatgaatg ttaaacggag cttaaactcg
     3721 gttaatcacg ttttgttcgt caataaacat acagcgattt cttccggttt gcttaccctc
     3781 atacattgcc cggtccgctc ttccaatgac cacatccaga ggctcttcag gaaatgcgcg
     3841 actcacacct gctgtcacgg taatgttgat atgcccttca gaatgtgtga tggcatggtt
     3901 atcgactaac tggcaaattc tgacacctgc acgacatgct tcttcatcat tagccgcttt
     3961 gacaataatg ataaattctt cgccaccgta gcgataaacc gtttcgtaat cgcgcgtcca
     4021 actggctaag taagttgcca gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt
     4081 atcattaacc aatttaaatc ggtcaatatc caacaacatt aaataaagat tcagaggctc
     4141 agcgttgcgt aactgatgat caaaggattc atcaagaacc cgacgacccg gcaatcccgt
     4201 caaaacatcc atattgctac ggatcgtcag caaataaatt ttgtaatcgg ttaatgccgc
     4261 agtaaaagaa agcaaccctt cctgaaaggc gtcgaaatgc gcgtcctgcc agtgattttc
     4321 aacaatagcc agcattaatt cccgaccaca gttatgcata tgttgatggg cagaatccat
     4381 tagccgaacg taaggtaatt catcgttatc gagtggtccc agatgatcaa tccaccgacc
     4441 aaactggcac agttcataag aatggttatc cgttatttct ggcttactgg catctctcgc
     4501 gaccacgctg tgaaacatac ttaccagcca ctggtagtgg gcatcgatag ccttattgag
     4561 atttaacaag atggcatcaa tttccgttgt cttcttgatc attgccactc ctttttcaca
     4621 gttccttgtg cgcgctattc taacgagaga aaagcaaaat tacgtcaata ttttcataga
     4681 aatccgaagt tatgagtcat ctctgagata acattgtgat ttaaaacaaa atcagcggat
     4741 aaaaaagtgt ttaattctgt aaattacctc tgcattatcg taaataaaag gatgacaaat
     4801 agcataaccc aataccctaa tggcccagta gtttaggcca tcaggctaat ttatttttat
     4861 ttctgcaaat gagtgacccg aacgacggcc ggcgcgcttt tcttatccag actgccgcta
     4921 atgttgatca tctggtccgg ctgaacttct cgtccatcaa agactgccgc aggaataaca
     4981 acattaattt caccactctt atcgcgaaaa acgtaacggt cctctccttt gtgagaaatc
     5041 aaattaccgc gtagtgaaac cgaagcgcca tcgtgcatgt tttttgcgaa atcaacggtc
     5101 attttttttg catcatcggt tccgcgatag ccatcttcta ttgcatgagg cggcggtggc
     5161 gctgcatcct gttttaatcc gccctggtca tctgccaacg cataaggcat gacaagaaaa
     5221 cttgctaata caatggcctg aaatttcata ctgactcctt aattgcgttt ggtttgactt
     5281 attaagtctg gttgctattt ttataattga caaataagaa tactgccaat tgttataagg
     5341 catttaaaat caggcaacta gctgtcaaat attcagagaa ttttactcac taaagtaaga
     5401 agaagattaa agagtcttaa acatatttca gaacaatcag atttataaat ttgaaaatta
     5461 ttataacgga agggtataca aaaaaagtaa ttttgctggc gactgcgaaa cggcattcgc
     5521 attagcggtg aagtttgccc tcacccagcg gctgcaatta ctgctataac gaggctggtc
     5581 tggcgataaa tccaggccag ccattggtgg tgtttatacg ttcaagccac gatgttgcag
     5641 catcggcata atctgaggtg ccttaccgcg ccattgtcga tacaggcgtt ccagatcttc
     5701 gctgttacct ctggaaagaa tcgcctcgcg aaaacgccgc ccattttcac gcgttaatcc
     5761 gccctgctca acaaaccact ggtaaccatc atcggccaac atttgcgtcc acagataagc
     5821 gtaataacct gcggcatatc cgccaccaaa aatatgggcg aaataactgc tgcgatagcg
     5881 tggcggtata gcaggaagat ccatattttc cgccaccagc gcccgcaatt caaaatcatc
     5941 gacatcctgc attgcttcgt tttcttccag gcaatgccag cgcatatcga gaagtgcggc
     6001 gctaagcagt tcgctcatct catacccttt attgaacagg ctggcattac gcattttctg
     6061 ttgcagttca tcaggcattg ctgccccgct ctgataatgc cgggcgtagc gagcgaatac
     6121 ctgcggatgc gttgcccagt gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg
     6181 cgtgttggtg ccggaaagcg tggcataacg ctggcgggca aaaaggccgt gcagcgtatg
     6241 accaaattca tggaataagg ttatgacatc atcccagagt aacaacgcag gctccccagc
     6301 ggcggatttc tgataattgc agacgttata aattaccgga tgcgtttcat tcagcgttga
     6361 ttgctcaaca aaattgccca tccatgcacc gccgcttttt gaatcacggg cgaagaaatc
     6421 accgtaaaat aacgccagcc ccacgccatt atgatcaaaa atttcccaca cacgaacgtc
     6481 aggatggtag acaggaatat caaaacgttc gacaaactta ataccgaaga gctgattcgc
     6541 ggtccagaat acaccttcat tcaacaccgt gtttaattca aaatatggct tgagctgcga
     6601 ttcatcgaga tcgaatttct cacgtcttac ctgctcggca taaaatgccc agtcccacgg
     6661 ctgcgcgcta aacccgccct gctgcttatc aataaccgcc tgtatggaga ctaattcatc
     6721 gctcgcacgt tgacgcgccg ctggaacaat ttcccgcata aagttgagtg ctgcttctgg
     6781 cgtttttgcc atctgatcgg cgattttcca tgcggcataa tgagggaaac caagcagttt
     6841 cgcctgctgc gcgcggatct ccaccagacg ttgaatgata gcgcgggtat cattggcatc
     6901 atttttttcc gctcgcgtcc agcccgcagt gaacagtttt tcacgcgtcg cgcgatcgcg
     6961 cagttcagca agcgctggtt gctgggtggt attcagcaac ggaatcagcc atttgttatc
     7021 cagacctttc tcgcgagccg cctctgccgc cagcgcaatc tcttgctcac tcattcctgc
     7081 cagctgcgcg aaatcgttca caaccagacc gccggattta tttgctgcca gtaaccgctg
     7141 gttaaactgg ctggtcaggg tcgcagcttc tgtattcagt acttttaatt ttgctttatc
     7201 agcttgtgca agtttggctc cggcaaggac aaaacgttga tgaatgacct ccaccaggcg
     7261 gatggattca ctatcaagtc ccagggattc acggcgttgc cagacagcat ctacccgcgc
     7321 gaataattca ccgttcagat agatatcatt agccagttcc gccagttcag cggaaaactg
     7381 ttcatcaaga cgctgtaatt catcattagt atgcgccgca gtcatcgcaa aaaagacgct
     7441 ggtaacgcgg gtaagtaatt ctccgctttg ttccagtgcc agaatagtat tgttgaaatc
     7501 aggcgcttgc gggttaagcg cgatggcagc aatttctgcc cgcttttgct gcattccctc
     7561 atcgaatgcc gggcgatagt gatgattggc aatttgatca aaatggggag ccagatacgg
     7621 cagtgtgctt tgcacaagga aaggattcat tgttgtcatt ttcttctcct gaacgcgagg
     7681 tgttccatag cgtaggctta ctgataacgg agtgcaatct tgcaaaccag tattacccgc
     7741 tcttaagcat cacgtgctat gttattgaca cacaaaagcg ttgaggaaca gtgagatgat
     7801 cgttttagta actggagcaa cggcaggttt tggtgaatgc attactcgtc gttttattca
     7861 acaagggcat aaagttatcg ccactggccg tcgccaggag cggttgcagg agttaaaaga
     7921 cgaactggga gataatctgt atatcgccca actggacgtt cgcaaccgcg ccgctattga
     7981 agagatgctg gcatcgcttc ctgccgagtg gtgcaatatt gatatcctgg taaataatgc
     8041 cggcctggcg ttgggcatgg agcctgcgca taaagccagc gttgaagact gggaaacgat
     8101 gattgatacc aacaacaaag gcctggtata tatgacgcgc gccgtcttac cgggtatggt
     8161 tgaacgtaat catggtcata ttattaacat tggctcaacg gcaggcagct ggccgtatgc
     8221 cggtggtaac gtttacggtg cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg
     8281 tacggacctg catggtacgg cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg
     8341 caccgagttt tccaatgtcc gctttaaagg cgatgacagt aaagcggaaa aaacctatca
     8401 aaataccgtt gcattgacgc cggaagatgt cagcgaagcc gtctggtggg tgtcaacgct
     8461 gcctgctcac gtcaatatca ataccctgga aatgatgccg gttacccaaa gctatgccgg
     8521 actgaatgtc caccgtcagt aatttttata cccggcgtaa ctgccgggtt attgcttgtc
     8581 acaaaaaagt ggtagactca tgcagttaac tcactcacaa gcaagaacga atgaccgtcg
     8641 aaacgcaact taatcccaca cagcctgtca atcagcagat ttatcgtatt cttcgtcgcg
     8701 acattgtcca ttgcctgatt gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc
     8761 gtttcaatgt gtcacgccag ccggttcgtg aagcctttat taaactggcg gaaaacggcc
     8821 tgattcaaat tcgtccgcag cgtggcagct acgtcaacaa aatttccatg gcccaggtgc
     8881 gcaacggcag ttttatccgt caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca
     8941 tgattaccga aagccagtgc taccaactgg aacaaaatct tcaccagcaa cgcattgcca
     9001 tagagcgcaa gcaactggat gatttttttg aacttgatga caacttccat caactcctga
     9061 cgcagattgc cgactgtcaa ctggcgtggg ataccattga gaacctgaaa gcgaccgttg
     9121 atcgcgtgcg ctatatgagt ttcgaccacg tttctccacc agaaatgctg ttacgccagc
     9181 atcttgagat tttctctgcc ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga
     9241 cgcaccattt gcaggaaatc agcgaatccg tgcgccagat ccgtcaggaa aacagcgact
     9301 ggtttagcga agagtaattc atttcctctc atcccatccg gggtgagagt ttttccctcc
     9361 gtcatatggc tcatgcatgc atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt
     9421 tcactcgaca ggagtattta tagtgcgccc gttacgtggg cttcgactgt aaatcagaaa
     9481 ggagaaaaca cctatgacga cctacgatcg taaccgtaac gcaatcacca ctggcagccg
     9541 tgttatggtt agcggcaccg gtcacactgg caagatcctg tcgattgata ctgaaggtct
     9601 gaccgctgag caaatccgcc gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact
     9661 ggcacctctg gacctgattc gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg
     9721 gcattgcttt ttttacttca cggaatattt cgccacggtc gctttcgcac catgcgctaa
     9781 taaagacaag tacgcttccg tcacttttgc agtaaacaag ctattgtctg gcaaatcatc
     9841 accaaagatc gccttaatcg ccagcaatga ctggacgcgc gctttccctt cggcactact
     9901 ttgtacagcc ttctgaataa caggtaacag tgggtcactg atttctatcg gatttccctg
     9961 ttcatcaaca ccaccgacat aacgcatcca acctgcgacg cccagcgcca gcagatcgaa
    10021 cttgctgtca tgcgccagat gccagcgaac agaatccaac atccgctgtg gcaatttctg
    10081 gctaccatcc atcgcaatct gccaggttcg atgacgtagc gccgggttgc tatagcgttc
    10141 aattaatcgg ttagcgtaat cttgcaaatc aacgccctgc actttcaacg tcggcgcttg
    10201 ttcctgcaac atcaaggcat acgctgcatg acgataatgt tcatcttcca tgcagtcatt
    10261 aatgtgctga tatccggcaa gataccccag atacgccagg aatgaatgac tgccgttgag
    10321 catgcgcaac ttcatctctt cataaggcag cacatcacta accagttcgg ctcccgcttt
    10381 ttcccattcc ggacgtccgg caacaaagtt atcttctatt acccactggc ggaaaggttc
    10441 acaggcaacg cccgcaggat cgcgcacacc ggtaagttgt tcgattttcg ccagcgtatc
    10501 ctccgtcact gcgggcacaa tacggtccac cattgttgat gggaaagtca cgttttcttc
    10561 gatccattgt gccagtttta catcaacagc ttgcgcgtag gaagtgacaa cgtcacgcat
    10621 aacatgaccg ttttctggca tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc
    10681 cgctttacgg cgagccagcg cctcgacaat cacccctgtt gcagttttcg gctggtgggg
    10741 attttgtacg tcggcaacga ccatcgggtg atcgagcatt aactgtccgg tcgccggaga
    10801 gtggaaatac cctttttcgg tgattgtcag agagacaatc gcgatttgcg gttcacacat
    10861 cacagccaac acggtttcta agccatcaat ctgtacgtgc aaggcttttt taacgacgcc
    10921 aacgacgcga gccgtccacg catcggccga catttccgca acggtataaa gattatcttg
    10981 ctgttgtaaa tcggcaattt gctgttcgcc gccgattaaa ttgacctcat aatatcccca
    11041 gtcactgaaa tgttccgtag caagaatatc ggcatacaca ccctgatgcg cacggtgaaa
    11101 tgcaccaaag cctaaatgaa caattcttgg ggccaggtta ttacgatcat aaacagggag
    11161 cgtcgctttt gctgataaca aattatttcc cataacaatt ccttaaattt aaatatggca
    11221 agctgcatgt tttgttatat gaataaaaaa tcccctctcc ggtaagagaa gggatggagg
    11281 atttacagac ttcttgaagg ttgcgcagct gttacaacac gcggttgatc ttccgcggca
    11341 tcttccagtg cacttaaatc acggtctttc acttctggca ttttcagcgc agagattaaa
    11401 ccaatcactg aatatgccat gatcataatg gcgatcggat accaggattc cgtcatggtg
    11461 cagaaaatac ccgccaggat aggaccaaaa ccggaagcga taagaccacc aatttcttta
    11521 gaaatggcca tccgggtaaa gcggttttta cagccgaaca tttctgccat ggtaatgttt
    11581 tccagagcaa ataatcccag caccgcacag ttatgaatca caatcagtgc caccataatg
    11641 gtgctcgggg catagctttt atctacgatg atagaaagca ttggccatgc cagcacaatc
    11701 gcggaggtat tcataataat atacgggatc cggcgaccaa ttttatcgga taaccaacca
    11761 aggaacggaa tggtcataaa gccgagaatc gaactgatca tcaatgcatc tgttggaatt
    11821 gctttgttaa acaataacgt ctgtactaaa tagcctgcaa ggaaagtctg aattaaaccg
    11881 gagttaccag cctgaccaaa acgcagccct gttgccagcc agaaggattt gctctggaac
    11941 atgctaccag caggtgcagg ttttgctgtc ggttggttgc tgtcgttaac cttctcaaag
    12001 accgggcttt ctttcagatt catacgcaac cagatagcaa agaccatcac gacaacgctc
    12061 gccaggaacg gtatacgcca tccccacgcc aacagttcct ctttactgag aatgaagaac
    12121 ataaaggccc agattgccgt tgcgcttaag gttccgcagt tagttcccat agccacaaat
    12181 gaggagataa ttccgcgctt accttttggc gcatattccg ccagcatcgt accggcaccg
    12241 gaaatttccg cacctgcacc caatccctga ataatacgca gcgtcaccag caagatgggt
    12301 gcaaaaacac caatctgtgc ataggtcggt aacacaccaa ttaaggtggt acagatcccc
    12361 atcatggtga tggtaataaa gagtactttt ttacgcccta ttctgtcgcc cattttgccg
    12421 aaaataaatg ctccgacaat acgcgccaca taacctgcac cgtaggttcc cattgccaga
    12481 attaacgcca ttgccgttga tgattcagga aaaaatattt catgaaacac taacgctgca
    12541 ccgagcgaat ataactggaa atccataaat tcaagcgcag tgcccagcca tcccgatact
    12601 gctgctttca ccaaatcctt agtgcttctt tcgtgttttt ctattgtcat aatggttatc
    12661 tctaaaaaag aggtaagatg cgtactactt actcgccgtt attggtatta ttcagaaaaa
    12721 gtaagtaaga ctttgcagca atgtttttga tcctgttcaa ataaactaat ggcatcagca
    12781 acatgctgga aatcaaacgt atgggtaatt aatttttctg gtttaattaa ccctttactt
    12841 aaccagtcga taacgaccgg aaatttattt gcatttaagc gtgaagagaa aatagagagt
    12901 tcttttccgg taattccttg ctgaatcact tcagacggtt cactggagaa gcccatcaat
    12961 acaatacgtg ccgctggaga agccagcgtt acagcctctt tcaggataga aggatgacaa
    13021 gccgcatcga taattaatgt cggcttgatg cctttttcag cgaaactctc gccaagcggt
    13081 gtctggctgt tattaatcgc ccagtctgcc ccgctctctt tcgctttttc cagtcgttca
    13141 tcaatgcgat cggcaacaat cacattttta acgttataga cgccttttaa tacctgaacg
    13201 atcgtcaggc cgattggacc ggcaccgtaa accagaacgg tatcattttc agtcggttga
    13261 ccatgaccgg taacgttagc cgcaatggta aaaggttcga tcatcaccgc atattgatcg
    13321 gccactgctt caggaatttt ccacgcattt tttgccggaa ccacggcata ttcactgaaa
    13381 ccaccgtcag cgtgcacacc taatacagcc agtgtcgtac aaacgttcgg cttacctata
    13441 gagcacggat agcaatgccc acagctgacc accggatcga cagcaacgcg ttcaccgact
    13501 ctggcgcttt ccacgccttc acccactgca tcaatgacgc caaagaattc atgaccaatg
    13561 acgcgcggat atttcgcaaa aggattatgc ccacgataaa tatggctatc tgaaccacaa
    13621 attccggcaa gtttcacttt tactcgtact tcacccgctg acggggtggg tatttcgcgt
    13681 tcgataattg ccagttgatt cggtttttca attaaaatgc ttttcattat cttactcctt
    13741 accagttcca cagcgtgcca tcttccagac gtgcgactgg cagataagca ggttcatagg
    13801 gatatttcgc cgccagcttt tcatcgaatt cgatgccaag acccggtttg tctcccggat
    13861 gcatatagcc gttatcgaaa gtccagttgt gcgggaagac ttcaagcatt tgttcggaat
    13921 aacccatgta ctcctggaca ccgaaattgg ggacccacag atcaaagtgc agcgccgcag
    13981 ccatgcagac tggcgacaaa tcggaaggac cgtgtgagcc agtacgtacc tgatacagcg
    14041 aagcaaaatc ggcaatccgg cgcataccgg taatcccccc cgcgtgagtc agcgtggtgc
    14101 ggatatagtc gataagttgt tcttcaatca gctgtttgca gtcccagatg ctgttgaaga
    14161 cttcacccac tgcaattggc gtgacggtgt gttggcgaat caggcggaaa cactcctggt
    14221 tttccgcagg cgtcgggtct tccatccaga acatgcgata atcttcaatg cttttaccaa
    14281 agcgcgccgc ttcaataggc gttaagcgat ggtgcatgtc atgcagcaaa tgttcattaa
    14341 aaccaaactt gttacgtacc gcgtcaaaca atttcggcat gaaatcgagg tatttctccg
    14401 tcgaccacag ctgctcttcc ggccactgtc ctttggttgc gggttcataa gccagacctt
    14461 tacctttcga catgccatag gtggttttca taccagggat tccgcactgc acgcggatgg
    14521 ctttgaatcc cagctcctga tgacgggcat aatcatccag agcttcatca atactgtgac
    14581 cggtggtatg gcaataaacc atcacccctt cacgagacgc gccgccgagt aactggtaaa
    14641 gcggcatgtt ggcagctttg gctttaatat cccacagcgc catatcaacc gctgaaatgg
    14701 ccgacatcgt aaccggaccg cgacgccagt aagcaccttt atagaaaaac tgccagatat
    14761 cttcgatacg gtgtgcatcg cgaccaataa gctgcggaca aaggtgatcc tgcaaataag
    14821 aggccacgga aagctcacgt ccattgaggg tggcatcccc aaggcccgta ataccgtcct
    14881 cagtggtgat ttttaatgtg acgaaattac gccccggaca ggtaacaaaa acttcagcct
    14941 ttacgatctt catgttcgat tccttgcatc gcttgtcgtg atgcatgaaa tctacgcaac
    15001 tgagctacta ccatacaagt atgaagatca aaaaaagccg gagtgatcac aaaaaaaggc
    15061 gtatatttgc gccgtgaatg gttgacaaaa gatgaaatag aatacctttt gtcagctgac
    15121 acttcctcgt atcttattga taaaagggat ttatgttcct acgtgcgccc ccagcccgca
    15181 acaatgatca acatgccgca aagcgcaatc aacgcccccg tccagtcata aagactcagt
    15241 ttcacgccat ccacaacgcg cagccacatc aacgccgtgc agacataaac gccaccataa
    15301 gcggcgtaaa cacgcccact cgccgcagga tgcaacgtta acaaccagac aaacagcgcc
    15361 agtgaaatcc ccgccggaag caacagccag atactggcgt ttcgttttaa ccacaaccag
    15421 ggcagaaagc atccaataat ttcacacagc gcagtagcaa aaaatagtaa cgttgtttta
    15481 atcatctttg tcacttattg acatcatgta tagttatagg acgacataat atcatcaata
    15541 taaacatcct cctggtacgt tttgcgttcg cagtggatgg tgtagaatca cctttaatca
    15601 ttcatacagg gaatgaatta tgaaaattac tctcagcaaa cgaattggcc tgctcgcttt
    15661 tctgctgcct tgcgcactgg cattgagcac aactgttcat gccgaaacta acaaactggt
    15721 gattgagtct ggcgacagtg cacaaagccg ccagcacgcc gctatggaaa aagagcaatg
    15781 gaatgacacg cgcaatctgc gccagaaagt gaataaacgc actgaaaaag agtgggataa
    15841 agccgacgcc gcttttgata accgcgataa atgtgagcaa agcgccaaca tcaatgccta
    15901 ctgggagcca aatactttgc gctgcctgga ccgtcgaact ggccgcgtta ttacccccta
    15961 acctgttatt gatttaagga atgtaaggac acgttatgcc aagcgcccac agtgttaagc
    16021 tacgcccgct ggagcgtgaa gatttacgct atgtacatca actcgacaat aacgccagtg
    16081 tgatgcgtta ctggtttgag gaaccctacg aagcctttgt tgaactctct gatctgtacg
    16141 ataagcatat tcacgatcag agcgaacggc gctttgtggt ggaatgtgac ggcgaaaaag
    16201 ccggtctggt ggagctggtg gaaattaacc atgttcatcg ccgcgcagaa tttcagataa
    16261 ttatctcccc ggaatatcag gggaaaggtc tggcaacgcg tgccgcaaaa ttagcaatgg
    16321 actatggctt caccgttctc aatctctata agctgtatct gatcgtcgat aaagagaatg
    16381 aaaaagcgat tcacatttac cgcaagcttg gctttacggt tgaaggtgaa ttgatgcatg
    16441 agttctttat taatggtcaa tatcgtaatg ccattcgcat gtgcatattc cagcatcagt
    16501 atctggcaga gcacaaaaca ccgggtcaga ctctcctgaa gccgaccgca caatagcatt
    16561 aataataatc gatagtattt ttgatggtgt aaacccgttc gacggcgggt tttactcctt
    16621 catcaacaat gattagctga cagtccaccg gattggcgtg actgtcatat tcacagctct
    16681 gttttacatt accaaccctt tgattattca gtaaagtaac cgctgtgtaa tctaattttt
    16741 tgatcggatc cgttgatggc gtagcgctga cagataatgt tttgtcgtta cttttcgtgg
    16801 ttttacccag cggatacccc tgatcatcat agcgatattc catctgcatc tgtttgctgc
    16861 tggctttaat cacaaagcca ttatcatcgg tttcccagct caccccggca gaaggtaatt
    16921 ctgctagctg gcattttccc tgcaaacgaa ctctcttctc cagtgtctgg gcatcacggt
    16981 aataattggc gtccagtacc agggcaacca cggtattatt ttccagatcc agtaattcga
    17041 gtgaatcaaa acagccttct tccgacaaag tccccgaaac acgtttcgtc acttcacctt
    17101 gctcatccat taatgtctga gtgaaatctt ttaccggacc acgcagcgga tcaaaatcga
    17161 attcattaga gaaactggcc atctcagggg taaatgaaag tgttacctct gtgcggtcac
    17221 atcctgtgag gaatatcgcg agtaagcatg gcagtaattt gtatttcaca acagtcacca
    17281 gagagtagag atgattctca atcatagtag caaatacagt actttacacg ttaaatgcta
    17341 tgcttaaaga agttatcttc gcgtaaggag cttatgatga aactctcaac ctgctgtgcc
    17401 gcgcttctgc tcgccctcgc ctcacccgcg gtactcgccg cgccgggatc ctgtgagcgc
    17461 atacagagcg atatatcaca gcgcattatc aataatggcg tacccgaaag cagcttcacg
    17521 ttaagtattg tacccaatga ccaggttgat cagcctgatt cccaggtcgt cgggcattgc
    17581 gctaatgata cgcataaaat tctctacacc cgcaccacca gcggtaacgt ctctgctccc
    17641 gcgcagtcta cccaggatgg cgcgcctgcc gaaccgcagt aatacattat cgactgcacg
    17701 ccggatacga cacatccggc gatttgaaca tacaacataa tcccacctta ttactcatac
    17761 ccttctattg atatggatta ataattctta acccaaaata ggtagactcc ctctattgtt
    17821 agcgcgctaa atattcaata tataaacttt tatataacga taaagaacag ggagtgagtt
    17881 atgtccaaaa atgaacaaat ggtgggcatc agccgcagaa cactcgttaa atctaccgcg
    17941 ataggttctc tggcgctggc tgcaggcggt ttttctttac cgttcaccct gcgcagtgca
    18001 gcggcagcgg tacagcaggc cagcgagaaa gtggtctggg gtgcctgttc cgtcaactgt
    18061 ggtagccgct gtgcacttcg tctgcatgtt aaagataatg aagtgacctg ggtggaaact
    18121 gacaacaccg gcagcgatga gtatggcaat catcaagtac gcgcctgttt gcgcggtcgc
    18181 tccatccgtc ggcgtattaa tcatcccgac cgcttgaatt acccaatgaa acgcgtgggc
    18241 aaacgcggcg aaggcaaatt cgaacggatt agctgggatg aagccctgga tactatcgcc
    18301 agtagcctga aaaaaaccgt cgaacaatat ggcaatgagg ctgtatatat tcagtactct
    18361 tcagggatcg ttggcggcaa tatgacccgc tcttcgccat ccgcctcggc ggtcaaacgc
    18421 ctgatgaact gctacggcgg ttcactcaac cagtatggct cctacagcac cgcgcaaatc
    18481 tcctgtgcca tgccctacac ctacggcagt aatgatggca acagcaccac ggatattgaa
    18541 aacagcaagc tggtcgtgat gtttggtaac aacccggctg aaacccgcat gagcggcggt
    18601 ggcatcactt atcttcttga aaaagcgcgc gagaaatcga acgcaaaaat gattgttatc
    18661 gatccgcgat ataccgatac ggctgctggt cgtgaagatg aatggcttcc tattcgcccg
    18721 ggcaccgatg ccgcgctggt tgcgggtatt gcctgggtat tggttaacga aaatctcgtt
    18781 gatcaacctt ttctcgataa atactgcgtc ggctatgacg aaaaaacctt acccgcagat
    18841 gcacccaaaa atggtcacta taaagcctat attcttggtg aaggtgatga taaaacagcg
    18901 aaaacgccgc agtgggcttc gcaaattaca ggtatcccgg tagaccgtat catcaaactg
    18961 gcgcgtgaaa ttggcacagc aaaacccgct tatatctgcc agggctgggg gccgcaacgc
    19021 caggcaaacg gcgagctgac ggcacgcgct attgctatgt tgcctatttt gacgggcaat
    19081 gtcggcatca gcggcggaaa tagtggtgcg cgtgaatcga cctacaccat taccatcgaa
    19141 cgcctgccgg tgctggataa tccggtcaaa acgtcaatct cctgcttcag ttggacagat
    19201 gctatcgatc atggcccgca aatgacggca atccgcgacg gcgtccgggg caaagataaa
    19261 ctggatgtgc ccattaagtt catctggaac tacgcgggaa ataccctggt taatcagcat
    19321 tctgacatca acaaaacgca tgaaattctg caggacgaat cgaaatgcga aatgattgtg
    19381 gtcatcgaaa actttatgac ctcatcggca aaatatgccg acattctgct gccagacctg
    19441 atgaccgttg agcaggaaga tattattcct aacgactacg ccggtaacat gggatatctc
    19501 attttcctcc agcctgtcac cagcgaaaaa ttcgaacgca aaccaattta ctggatcctg
    19561 agtgaagtcg cgaaacgtct tggacctgac gtctatcaaa agttcactga aggtcgcacg
    19621 caggaacaat ggttacaaca tctgtacgcc aaaatgcttg ccaaagatcc ggcgttaccg
    19681 tcttacgacg aattaaaaaa aatgggtatc tataagcgta aagatcccaa tggtcatttt
    19741 gtcgcctaca aagcatttcg tgacgacccc gaggcaaatc cacttaaaac gccttccggt
    19801 aagattgaaa tttattccag caagctggcg gaaattgccc gtacctggga actggaaaaa
    19861 gatgaagtga taagcccact gccggtttat gcctcaacct ttgaaggctg ggactcccct
    19921 gaacgtagta cctttccact gcaactgttt ggtttccatt acaaatcccg tactcactcg
    19981 acctacggca acattgatgt cctgaaggct gcctgccgtc aggaggtgtg gatcaaccct
    20041 atagacgcac agaaacgtgg gattgccaac ggcgatatgg tgcgggtgta taaccatcgt
    20101 ggcgaagttc ggctaccagc caaagtgaca ccacgcattc tccctggggt tagcgctatg
    20161 ggccagggag cctggcacga ggccaatatg tctggcgata aaatcgacca tggcggctgt
    20221 gtgaatacgc taaccactct gcgcccttca ccactggcga agggaaaccc gcagcacact
    20281 aatctggtcg agatcgaaaa aatataaccc actacaacca taaattctgg catgacattt
    20341 tgtttgaaaa gcaataagtg agtaatgatg aaaatccata ccacagaggc gctcatgaag
    20401 gctgaaatca gccgtagaag tctgatgaaa acctccgcac ttggcagtct cgcgctggca
    20461 agcagtgctt tcactctgcc attttcccaa atggtccgag cggcagagac accggtagaa
    20521 gagaaagcgg tctggagttc ctgcaccgtt aactgcggga gccgctgtct gttacgtttg
    20581 catgtgaaag atgacaccgt gtactgggtg gagtctgata cgacaggaga cgacgtttac
    20641 ggtaatcatc aggttcgggc atgtttacgc gggcgctcta tccgccgacg gatgaatcat
    20701 cctgataggt tgaaatatcc catgaagcgc gtcggcaagc gcggtgaagg taaatttgaa
    20761 cggataagtt gggatgaagc cctggatacc atcagtgata atcttcggcg gatccttaaa
    20821 gattacggca atgaggctgt acatgtcctg tacggaacag gcgtagatgg cggaaacatc
    20881 accaactcga acgtcccgta ccgtctgatg aactcttgcg gtggttttct cagtcgctat
    20941 ggcagctaca gtaccgctca aatcagtgcc gcaatgagtt atatgttcgg tgccaatgat
    21001 ggcaacagcc cggatgatat cgccaatacg aaactggtcg ttatgttcgg aaataacccg
    21061 gcagaaaccc ggatgagcgg cggtggtgtc acttactacg tcgagcaagc ccgagaacgt
    21121 tcaaacgcac gcatgatcgt catcgatcca cgttataacg acacagctgc cgggcgtgaa
    21181 gatgaatggc tgccaattcg cccaggcacc gatggcgcac tggcctgtgc gattgcctgg
    21241 gtactgatta ctgaaaacat ggtcgatcag ccatttctcg acaaatattg tgttggttac
    21301 gatgaaaaaa cgctgcccgc caacgcacca cgtaacgcgc attataaagc ctatattctg
    21361 ggcgaagggc ctgacggcat agctaaaacg ccggaatggg cagcaaaaat caccagcatc
    21421 ccggcagaaa aaattatcca gttggcacga gagatcggtt cagcaaaacc tgcttatatt
    21481 tgtcagggtt gggggccaca acgacactcc aacggcgaac aaacgtcccg cgctattgcc
    21541 atgctttccg ttctcaccgg taacgtcggc ataaacggcg gcaactcagg tgtacgcgaa
    21601 ggtagctggg atctgggggt agaatggttc ccgatgcttg agaatcctgt taaaacgcag
    21661 atttccgtct ttacatggac agatgccatc gaccacggta cggaaatgac cgcgacccgt
    21721 gatggtgttc gtggaaaaga aaaactggat gtccccatca agtttttatg gtgctacgcc
    21781 agtaacacat tgatcaatca acatggcgac atcaatcaca cccatgaggt gcttcaggat
    21841 gacagcaagt gcgagatgat tgttggcatt gaccacttca tgactgcctc ggctaagtat
    21901 tgcgatatcc tgttgcccga cctgatgcca acagagcaag aagaccttat ctctcacgaa
    21961 tctgccggga atatgggcta tgtgatcctc gcccaacccg caacctcagc aaaatttgaa
    22021 cgcaaaccca tctactggat gctgagcgaa gttgccaaac gcttaggacc agacgtttat
    22081 caaaccttta ctgaaggccg cagtcagcac gaatggatca aatatctcca tgcgaaaacg
    22141 aaggaacgta accctgagat gcccgactac gaggagatga aaacgaccgg gatctttaag
    22201 aaaaaatgcc cggaagaaca ctacgtcgct ttccgcgcat tccgtgaaga tccacaggca
    22261 aacccgttga aaacaccttc ggggaaaatc gaaatttatt ctgaacgact ggcgacgatt
    22321 gcggatacct gggaattgaa aaaagatgaa attattcatc cccttcctgc gtatacccca
    22381 ggttttgatg gctgggacga tcccctgcgg aaaacctatc cactgcagct aacgggcttc
    22441 cactacaaag cgcgtaccca ctccagctac ggcaatattg atgtgttaca gcaggcctgc
    22501 ccacaagagg tgtggatcaa ccctattgat gctcaggcac gcggtatccg tcatggcgat
    22561 accgtgcggg tatttaacaa taatggagaa atgctgattg ccgcaaaagt gactccgcgt
    22621 attctgcctg gcgttaccgc catcgggcaa ggtgcgtggc ttaaagcgga tatgtttggt
    22681 gaccgggtcg atcacggcgg cagtatcaat attctgacct ctcaccgccc ttcaccgctg
    22741 gcaaagggaa acccgtcgca cagcaatctt gtccagatcg aaaaggttta aggagtagcc
    22801 catgaccaca caatatggat tttttatcga ttccagccgc tgtactggct gtaaaacctg
    22861 cgaactggcg tgcaaagatt ttaaagatct tggcccggaa gtcagtttcc gccgtattta
    22921 cgaatatgct ggcggcgact ggcaggaaga taatggcgtc tggcaccaaa acgtgttcgc
    22981 ttattatctc tccatctcct gtaaccattg cgatgacccc gcatgtacaa aagtctgccc
    23041 cagcggcgca atgcataagc gtgaagatgg ctttgtggtg gttgatgagg atgtttgtat
    23101 cggctgccgc tactgccaca tggcttgtcc gtacggcgcg ccacagtaca atgctgaaaa
    23161 agggcacatg acgaagtgcg atggttgtta ttcgcgcgtc gccgagggga aacaacccat
    23221 atgtgtcgaa tcctgcccgc tgcgtgcact tgagttcggt ccaattgaag agctgcgcca
    23281 gaagcacggc actctggctg ccgtcgcgcc actaccgcgc gcgcatttca caaaacccaa
    23341 tatcgttatc aaacctaacg ccaacagccg cccgaccggc gataccactg gttatctggc
    23401 taatccggag gaagtgtaaa atgggaaatg gatggcatga atggccactg gttatcttta
    23461 ccgtattggg tcaatgcgta gtaggcgcgc tgattgttag cggaattggc tggttcgccg
    23521 ccaataatga cgcagaccgt cagcgtattg tccgtggcat gtttttcctc tggctgttga
    23581 tgggcatcgg gtttattgcc tctgtcatgc acctgggatc gccactgcga gcgtttaact
    23641 cgctgaatcg aatcggagct tctggtctga gtaacgagat tgctgccggt tccatatttt
    23701 ttgccgttgg cggattatgg tggctggtgg cagtcatcgg taaaatgccg caagcgttgg
    23761 ggaaactctg gttacttgtc agcatggcgc tcggcgtcat tttcgtctgg atgatgacct
    23821 gcgtgtatca aatcgacaca gtgccaacct ggtataacgg ttacactacg ctggctttct
    23881 tcctgacagt attgttgagc ggtccaatac ttgctgccac aattctgcgg gcagcccgcg
    23941 ttacatttaa taccacacca tttgccatca ttagcgttct ggcgctgatc gcttgtgccg
    24001 gggtgattgt gctgcaaggt ttgtctctgg cgtctattca ctcatccgta caacaagcca
    24061 gcgcactggt accagattac gcctccttac aggtctggcg tgtggtattg ttatgcacag
    24121 gtctaggatg ctggctttgt ccgctcattc gtcgccgtga acctcacgtt gccggactta
    24181 ttctggggct aatcctcata cttggcggtg agatgatcgg gcgtgtgctc ttttatggcc
    24241 tgcacatgac ggtaggaatg gctattgcag gttaacaaat tggtgcgggg caacccgcac
    24301 atttcaggat gttaggaatg acccattttt cacagcaaga taatttttct gtcgcggcgc
    24361 gcgtgttggg cgcgctgttt tattacgctc cagagagtgc ggaagccgca cctctggttg
    24421 cggtactcac ccgtgacggc tgggaaactc agtggcctct accagaagcg tcattagcgc
    24481 cgctggtgac ggcttttcag acacagtgtg aagagactca cgcccaggcc tggcagcgtt
    24541 tgtttgtcgg cccgtgggca ctgccgtctc cgccgtgggg atcggtctgg ctggatcgcg
    24601 aatctgtgct gtttggcgat tcaacattgg cacttcgtca gtggatgcgc gagaaaggca
    24661 ttcagtttga aatgaagcaa aacgaaccgg aagatcattt tggttcactg ctgttgatgg
    24721 ctgcgtggtt ggcggagaat ggtcgccaga cagaatgcga agaattgctg gcatggcacc
    24781 tttttccgtg gtcaacacga tttcttgacg tttttatcga aaaagcagaa caccctttct
    24841 accgtgcact gggggaactg gctcgcttaa cgctggcgca atggcaatca caactgttga
    24901 ttcctgtcgc ggttaaacca ctctttcgat aggatcaccg taatattgcc ggatggtggc
    24961 gattacgcgc aattcggcaa taataggtta cagtgtcacg tttttttatc tcttaaagca
    25021 cgcactgctt ttgcggctgg cctcttttgc cgcaaaatag tcgcccgtgt ttcattgccc
    25081 atttctgctc atgcatcatc tacacatcta tccggatctg cgcactatgt tccgccgtct
    25141 gcttatcgca acagtcgtcg gtattcttgc ggcctttgcc gttgccgggt ttcgtcatgc
    25201 aatgctgcta ctggagtggt tgttcctcaa taatgactcg ggcagtctgg tcaatgcagc
    25261 gacaaatctt tccccatggc gacggctgct aactccggcg ctcggcggac tggcggcggg
    25321 tttgttgctg atgggctggc agaaatttac ccagcaacgc cctcatgcgc cgaccgatta
    25381 catggaagcg ttgcaaaccg atgggcagtt tgattacgca gcaagcctgg ttaaatcgct
    25441 tgcctctctg ctggtagtaa ccagcggcag tgcaattggt cgcgaaggtg cgatgattct
    25501 tttagctgcc cttgccgcct cctgttttgc ccaacgtttt acgccacgcc aggagtggaa
    25561 attatggatc gcctgtggag ctgcggcggg aatggctgcg gcctatcgcg ccccgcttgc
    25621 tggcagttta tttatagccg aagtgctgtt tggcactatg atgttggcct ctctcggccc
    25681 ggtgattatt tccgccgtcg tggcgttgct ggttagcaat ctgattaatc atagcgacgc
    25741 gttactctac agcgtacaac tctcagtgac ggttcaggct cgtgactatg cgctgattat
    25801 cagtacaggt gtgctggcag gtctgtgcgg accactgttg ttaacgttaa tgaacgcctg
    25861 tcatcgtgga tttgtaagtc tcaaacttgc gccgccctgg caactggcac tgggcgggtt
    25921 gatcgtgggt ctgctttccc tgttcacacc tgcagtgtgg ggcaacggct atagcaccgt
    25981 acaatccttt ttaaccgccc cgccactgtt aatgatcatt gccgggatct tcctttgtaa
    26041 actgtgtgcc gtgctggcga gtagcggttc cggcgcaccc ggtggtgtct ttacaccgac
    26101 gctatttatc ggtcttgcca ttggcatgtt gtatggtcgt agcctgggat tatggttccc
    26161 tgatggcgaa gaaattacgc ttttactcgg attgaccggg atggcgacac tgttggcggc
    26221 aaccacgcac gcgccgatta tgtcgacgtt gatgatatgt gaaatgaccg gggagtatca
    26281 gctactcccc ggtttattga ttgcctgcgt aattgcgtcg gtaatttcgc ggacgttaca
    26341 ccgtgactct atctaccgcc agcacactgc gaagcatagc taagcggatg tattgcccca
    26401 gttcacgctg ttcggcgcgc ggcagataag gcagttcacc aatgagcggt gccggaagtt
    26461 ttttaccgag cacatcaatg atttccgcat aatgcgccag tcctggattg attcggttag
    26521 ccacccagcc aatgagcggc agcccgtcgt tggcaatcgc ctgagctgtt agtagtgcat
    26581 ggttaatgca accttcctga ataccgacaa ccatcaatac cggcagttgt tcctgcacca
    26641 cccattcaga gagcggacgc aaatcattca tcagactgcg ccagccgcca gtcccttcta
    26701 ccacgacatg atcgacttta tcggtcaggt ttgccaggcc gtttgaaatg agggtgtaat
    26761 tgattgggca actgtgcgcc acgctacttt cttcttcgct taacgcgata ggattaactg
    26821 cttcataagg cagttcgatg gttgaaacac tctgcaacac cagggcatct ttattacgca
    26881 gcccttcggg tgtctctttg ctccccttcg ctacgggttt atatcccgca accgtttttc
    26941 cctgggaggc taacgcttgt agcaatgcgc gggaaaccac cgttttccct acagaagtgt
    27001 ctgtaccggt aataaagaaa cgcttcagca tcactaactc caccgttatg cttcacaaat
    27061 ataaaccagg aaaataatta accttgaaag tctaagttat gctttcctgg cccaaattga
    27121 gatagcgcaa attttggtag aacagttaaa aaatgttaac cctgcaacag acgaatcaac
    27181 aaagaaccgt tatacatcgc gtcttttacc agcgcagccc ctgccatcgt accctggtta
    27241 gaaaactgag tactctcaac gctaatgtgc tgactatacg caggaagggc ctgctgacgg
    27301 atgctgtccg agatgaccgg gaagaggata tctgccgctt tacttaacgg tgagccaatc
    27361 agtatttttt gtgggttaaa taaattcacc atgatggcaa gaatgcgccc tacatgcgcg
    27421 cccaccccgg taatgatgtc ttttgccagt agatcgccgc gcaatgccgc ctgacacaat
    27481 gaatccacgg ttaacggctg tccatgtaac atcgagctca tggattgatt gagacgcagc
    27541 tgtgccagct caagaatact gtccacactg gcgatggttt cgaggcagcc gtgattcccg
    27601 caataacagc gtttcccata cgggtcgacc tgcgtgtgac ctatttccac gaggctactg
    27661 ctgcctgcgt gtagcagatg accatcggta atgacgcccg cccccacgtt gtgatcgata
    27721 accacctgaa tcacatcgcg cgccccgcgt gaggcaccaa acaaggcctc agccatcgtc
    27781 catgcgctga tatcatgctg aatataaacc ggtacgccgg tatgctgctc cagcgcctcg
    27841 ccgagcggca tctcttttac atcctcgtag aacggcatgc gatgtacaat accattttcc
    27901 gtatcaataa ttcccggcaa ggttatggca atcgaagtca gacgctcaag ttttttctgg
    27961 tggcggataa aaaactgatc gatatgggaa atgatacgat ccagcaatgg cgagtcatct
    28021 tttaacgcca gttcctgcga ctcctccact accagtttgc tgctcagatc gcgcaaagca
    28081 aggaaaatct ccccgcgact aatgcgcaga gaaagatagt gccaggcttc agtttcaacc
    28141 accagcccca ctgccggacg gccacggttc cccgcttctt tgatttccag ctcttgcacc
    28201 aggtgtgctt cgagcatctc acggacaatt ttagtgatac tggcaggagc cagctgcgcc
    28261 agacgggaaa gatcgatacg cgagactgga ccaagctgat caatcaggcg ataaaccgcg
    28321 cccgcgttgg tctgctttat ttgatcaatg tgcccaggct gattttcagc aaccaccgca
    28381 tactccctat attttcgcgc tccgaaataa tctgtaggct atggtgaagc acttcaatac
    28441 gtgtcgtcaa atttttactt aggcatgtga ttaacagcac atttttcggg ctttttcgct
    28501 gaaatttccc tcacctgaga gcattcagca gatgaatacg aaagttacgc gcagccggac
    28561 tttgttcatg gtgtttcggc cagaccaacc acatttcaga aaccgcatcc tcttcagcaa
    28621 tcggcaccca gcgcatttcg ttgagctgaa cacgtttaaa tgacgcaggc aaaattgaaa
    28681 cacccagacc agcggaaacc agaccgatga tggtcattgc ctcacccacc tcctgagtga
    28741 tgacgggcgt caaattgtaa cgtcgcatca gcccgagaat atcgtcatac agccctgtcc
    28801 ctacgtgcgg atcaaaaaag acaaagggtt ctttcgccag ttcagccagc gttacactcg
    28861 ggttatttgc caagggatga tcgtgcggga tcatcgccat aagcggttca tggacgatga
    28921 ctgcgtgttc aagcgtctcc ggtaacgctg tgttacgcag caatcccata tccagcgttc
    28981 cttcaatgag cggagcgatt tgctcgcggg tgttcatttc gcgggtttgt aaatggacat
    29041 cgggataatc acggcgaaac agcgataacg tatcggacac cgcccgaata aaaggagcgg
    29101 acgaagtaaa accaatgcgc aactcccccg cttcaccctg atgcagtctt tcagcgcgag
    29161 cggcagcgtc atccatcata gacaggattt gccgactatc tgcaagaaac tgttttcctg
    29221 ctgccgtcag caatacactg cgattagttc gtgccagcag tcgggcacca atttgttgct
    29281 ccagcgcctg aatctgctga cttagcggcg gttgcgaaat attcaggcgg gcagcggcgc
    29341 gcccgaaatg cagctcttct gcaacagcaa caaagtaacg cagatgacga agttcaatat
    29401 tcatatttaa aacatcttat ttgagattat taatatatta gacagaacaa ttcgattttc
    29461 ctaatctatt cataagcctg aaccacaggc atatttagca aggatttcaa gtgagccgta
    29521 ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt tctcagccaa
    29581 atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg ctgttctctg
    29641 ccggactggc aaccttcgca cttctctatt gtgtgcaacc tatccttccg gtgctttcgc
    29701 aggagtttgg cttaacgccc gcgaacagta gtatttcact gtccatttct acggcgatgt
    29761 tggctatcgg tttgctgttt actggcccgt tatccgatgc cattggtcgc aaaccagtga
    29821 tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg atgaccagct
    29881 ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc gtagcagctg
    29941 ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt tcgatggggt
    30001 tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagc ggtgtcttca
    30061 cggacttttt caactggcga attgcgctgg cggcaatcgg ttgtttcgcg ctggcctcgg
    30121 cgttaatgtt ctggaaaatc ctccctgaat cacgccattt tcgcccgact tcactgcgcc
    30181 ctaagacgtt attcatcaac tttcgtctgc actggcgtga ccggggatta ccgttattgt
    30241 tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac gtcggctatc
    30301 ggttgatgct ctccccctgg catgtcagcc aggccgtggt tggcttatta tcgctggctt
    30361 atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc tatgggcgcg
    30421 gtccggtgat gttgttttcg acgggggtta tgctgtttgg tttattgatg accttattca
    30481 gctcgctgtg gctgatcttt gccggaatgt tactcttctc agccggattc ttcgcagccc
    30541 actcagtagc cagcagctgg atcggccccc gcgcaaaacg cgctaaaggc caggcctcct
    30601 cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg ggtggtgttt
    30661 tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg ctggtcattg
    30721 ctctgctggt cgggacgcgt ttgcatcatc gtctgcacgc ctgaaaaaat aagtccggac
    30781 tgaggtaaat acccgtccgg acttattgcc tgctcaaacc aacgttaata gccatcctaa
    30841 aatagacgaa gcgccagcca attcccgcag cgcatctagt gtcatcagga ttataagtac
    30901 ccaaataaac ggattcattt tgctgtgtgt catttattac tgatgcgcag ttattctacc
    30961 gctttgtaag tagtaaaata gttaacacga tcaagactac tattattggt agatgaattt
    31021 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
    31081 ttacacgctg aaaccaacca ctcacggaag tctgccattc tcagggatat agttatttca
    31141 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
    31201 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ctgcctttgc tgcagagact
    31261 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgcctgcgaa aaccacacat
    31321 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
    31381 aaaaatgcca aagctgaaca gaaagctcct gaacaaaaag cgcaggcagc gaagaaacac
    31441 gccaagaaac acagccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
    31501 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta aacgggtaac
    31561 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
    31621 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgatcgtagt
    31681 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
    31741 ttaataatca taattaccca ccagagtgtg atatgcgtag aaccattgct gtagtgttgg
    31801 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
    31861 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
    31921 cgacccaatc accgtgggat gcggttggcc aactggaaac ggccagcggc aatttatgta
    31981 cagcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
    32041 caaagggtaa agccgataaa gcggtggcgc tgcgttttgt gtcaaataaa ggtctttggc
    32101 gctatgagat ccacgacata gaaggccgcg ttgacccgac actgggaaag cggttaaaag
    32161 cagatgggga tggttggatt gttcctcccg cagctgcgcc gtgggacttc ggtttgattg
    32221 tgctacgtaa tcccccttct ggcattacac cattgccgtt atttgaggga gataaagccg
    32281 cgcttaccgc cgcattaaaa gcggcaggtc gtaaagtgac tcaggcggga taccctgaag
    32341 atcatctcga tacgttgtac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
    32401 cggtgatgtc gcatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
    32461 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
    32521 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
    32581 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcatgtt catcaggcaa
    32641 gtttcaccat gatcattcca gccaacagca gcaccaggcc aatccagcct ttacgattta
    32701 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
    32761 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
    32821 cagccagcac cgccgccaga gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
    32881 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
    32941 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
    33001 cctgatttga tcaacacaat cccggcgacc agggtggtta acccggcaat tttcatcaga
    33061 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
    33121 tcccacagcg cataagctac gcctaaggcg atctttttaa cggcgaaaga gagaaatata
    33181 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
    33241 gcccatttca tggacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
    33301 caataaatat acattgtcct tctcctgcaa gagaattatt ttaattttcg cttaattcag
    33361 cgaaaccaaa gtaaagtggt caagcccgcg cttagcgcaa gttttggcag aagaaaggac
    33421 taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga ggtacgaaga
    33481 tgggtaaatg tagaaaataa cgtcctgaac aaattgtcca taatattaca attatccgca
    33541 gtgttgcttc tcgtcatcgc ggatgataat tgtcctcggt agttgaacac gcctgatttg
    33601 tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc ggtttgattt
    33661 tcgttatgat gacggagcga aaaagacatt attattagca aaggaagaaa aaacggggac
    33721 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
    33781 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
    33841 cgcattattt attgctgtta ttcttaaccc gctggtgcaa cacatggtcc gctggcgtgt
    33901 gccgcgtgta ctggcagtgt caattttgat gaccatcatc gtgatggcga tggtgttgct
    33961 gttagcttat ctgggttccg cgctcaatga gttgacgcgg acgttacagc aatatcgcaa
    34021 ctctattatg acgccactga aagcgattga accgttgttg caacgcgtag ggattgatgt
    34081 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
    34141 cttattgacg cagttatcta atgccatgtc atcaatcttt ttattgctgc tgacggtgct
    34201 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
    34261 ggttgaaggg atggcggcga ttcagcgtgc gattgacagt gtgtctcatt atctggtgct
    34321 gaaaacagcc atcagcatca tcaccggcct ggtcgcatgg gcgatgctcg ccgcactcga
    34381 tgttcgcttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tcccgaatat
    34441 tggttcagtt ctcgcggcaa tcccccctat tgctcaggta ctggtgttta atggcttcta
    34501 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
    34561 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
    34621 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
    34681 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
    34741 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
    34801 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
    34861 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
    34921 gcgctttcca cacttccagc acaggcatac cagcaatagg actcttcgga tcatcctgcg
    34981 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
    35041 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
    35101 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacgttga
    35161 taccacgagc gcgcagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
    35221 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
    35281 cttctgcggt gatttcgcgg tgctcgccca cttcctgatc atcgccagta gaagagccgt
    35341 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
    35401 tgtaagaaag gatagcgccc gaagaaccga ccagcgcacc ggtcacaatc agcagatcgt
    35461 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
    35521 ccaccactgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
    35581 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
    35641 atacaatcag cagcaggaag gaaacgacca gagccgccag gttcattttg tgacggtttg
    35701 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgaac
    35761 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
    35821 ccagaatcgg tgccattccc gcgtcatgat gcagatagct gttaaagcca accagcactg
    35881 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
    35941 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
    36001 caacattacc cgtatccggt cccaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
    36061 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
    36121 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
    36181 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
    36241 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc gccctggcca
    36301 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtgac cgacatcaac
    36361 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac acaggccagc
    36421 gcaaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgccaa ccagccgaaa
    36481 agaatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
    36541 tcggttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
    36601 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
    36661 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
    36721 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
    36781 taaccaatca ctttgacacc attttccgta gtgaagattt cacccggcac ggtgtattca
    36841 cagttgccgc cgttttgggc tgccaggtcg acaatcacac tacccgcctt catggagtca
    36901 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
    36961 atgatatcga cctcttttgc ctgggcggta aagagttcca tttccgcttt gatgaacgcg
    37021 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctctttaaa atccagctcg
    37081 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
    37141 cgcacaatcg cgccgagact gtttgcagcg ccaatggcgg ccagacccgc aacacccgca
    37201 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtaatttg cccggtaaag
    37261 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
    37321 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
    37381 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
    37441 aaactcacca gcgttgtccc aggattcagt aacgcaattt catcatctaa cggcgcattg
    37501 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
    37561 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
    37621 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
    37681 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
    37741 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
    37801 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
    37861 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
    37921 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg gcgttcgcat
    37981 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
    38041 agttgcttca gtagtattga tgctgatacg gctgtttttt aagcatagac ggccatttga
    38101 gcaggattaa aattggctta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
    38161 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
    38221 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
    38281 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
    38341 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
    38401 gccgtttctc gtcgcgctga taaagaaggt gccgcctctt tttatgttgt cgacacttct
    38461 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
    38521 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
    38581 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
    38641 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
    38701 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
    38761 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
    38821 ggacgtgcag ctctggctgc cggtggcgaa gctgcgaaga aagttgagat cccgggtgtt
    38881 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
    38941 ggcgggcgtt acactgtcac gctcccagac ggcactaaag tcgaagaact gaacaaagcg
    39001 accgcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
    39061 actgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
    39121 atcacccgcc agtggcaaga acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
    39181 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
    39241 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
    39301 aaactccatt tttatgcgtt ttgccctaat atttattctt cttgtcacgt tttaatcact
    39361 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
    39421 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
    39481 cactgctcat cggctggagt attactggtg ctggtatttt attactgacc tttgccatgc
    39541 tgatcctcac gcgcattcgt cccgaacttg acggcggtat ctttacctat gcccgcgaag
    39601 gatttggtga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
    39661 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
    39721 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat cgtcggtgca tcggctttat
    39781 tgtggattgt tcactttttg atcctgcgcg gagtgcaaac cgctgccagc attaacctgg
    39841 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
    39901 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cccgtttggg
    39961 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
    40021 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
    40081 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggc gtggtggcac
    40141 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg actgatggtg gaaatgatgg
    40201 gaccgtgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggtgcgtact
    40261 taagctggac catcatggcg gcagaagttc cgttcctggc agccactcat aaagcattcc
    40321 cgcgcatttt tgcgcgccag aacgcacaag ctgcgccatc cgcttcactg tggctgacca
    40381 atatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacat
    40441 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
    40501 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
    40561 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
    40621 cggggctgct ggttttcctg tacgcgcgta aaacgcatac ccatgataat gtactgaatc
    40681 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
    40741 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaagccc
    40801 aacccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcctggc
    40861 atttcattaa tcaaaagcaa ccggtgatta tcagctatcg gacgcactat cccgccattg
    40921 atggactgat taacgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    40981 tgatggcgtt tgccgatgaa gtactaaaaa gcacccatgg cctgcgtgct attttgcata
    41041 acgccagcgc gtggatggca gaaaaaccag gtacaccact gaccgacgtt ctggcttgca
    41101 tgatgcagat ccacgttaat accccatacc tactcaacca tgcactggaa agattactgc
    41161 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    41221 gtagcgacaa acatattgcc tatgctgcaa gcaaagcggc actggataat atgacccgct
    41281 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    41341 tgtttaatga gcatgatgat gccgaatacc gacaacaggc gctgaataaa tcactgatga
    41401 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagctgct
    41461 ttgtcaccgg acgcagtttc cctcttgatg gcggtcgtca tctgcgttaa tgcagcttta
    41521 tccagcatat aatcaacacc catgcgctta tcccccagca tgcaacagaa ccaacaaaag
    41581 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga cgccaggtaa acaaaatagg
    41641 gaataatcga ccacatgcta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    41701 tggcaacaat ataatgcgcg atgagcgcaa aggtcgggaa aagtggtatc agcccggcga
    41761 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    41821 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag ttatcagtag
    41881 aatacctgat gaaaccttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    41941 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    42001 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgtg
    42061 gaaccgcgcg gcgaccgggc cgaagaaact attctgcaag aaaatccgga tttggtgtta
    42121 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    42181 tggtccggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    42241 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgttagcg
    42301 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgagcaaagg tcttcaggaa
    42361 acgtctctga ctccctacaa agccctgcat ttcggcacgt tgaccatcga tcctatcaac
    42421 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    42481 tgggaattag ctacccatgc tgggcaaatc atggaccgcg atgcattgct gaaaaattta
    42541 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    42601 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgttcg taacaaaggc
    42661 tatctttttg cgcctcatgc atgggaataa gcgatgaaaa aactgtttat ccagttttac
    42721 ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt gtacaaattt
    42781 accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc gctgtatctg
    42841 atgcgcagcg aattgcgtga gatcccccca cacgattggg gtaagacgct gaaggagatg
    42901 gatttaaatc tctctttcga tctgcgagtc gagccactga gtaaatacca tcttgatgat
    42961 atttccatgc accggctgcg tggcggcgaa attgtcgccc tggacgatca gtacactttt
    43021 ttgcaacgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc ttatctttat
    43081 tacctccatc agatgcgatt actggatatc gccctgatcg cttttattgc catttccctc
    43141 gcctttccgg tgtttatttg gatgcgtccg cactggcagg atatgttaaa actggaagca
    43201 gcggcgcaac gatttggcga tgggcatctc aatgaacgta tccactttga tgaaggttcg
    43261 agctttgaac gacttggcgt cgcatttaac cagatggcgg acaatatcaa cgccttgatt
    43321 gccagcaaaa aacagcttat tgacggtatc gctcacgaac tgcgaacacc gttagtgcgc
    43381 ctgcgttatc gactggagat gagcgataac ctgagcgccg tcgaatccca ggcgttgaat
    43441 cgcgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc ccgactcgat
    43501 cgcccacaaa atgagcttca tcttagcgaa ccagacctgc cgttgtggct gtcaacgcat
    43561 ctggcagata ttcaggcagt aacgcccgat aaaacggtac ggataaaaac gctcgcgcaa
    43621 ggccattatg ctgcgttgga tatgcgctta atggagcgcg tgctggataa tttgctcaat
    43681 aacgccctgc gctactgtca ctcaacggtt gaaaccagcc tgctactgtc ggggaataga
    43741 gcgacattaa ttgttgagga tgatggccca gggattgccc ctgaaaaccg cgaacatatc
    43801 tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg ctgcgggctg
    43861 gggctggcaa ttgtccactc tatagcactg gcaatgggcg gtacggttaa ttgtgacacc
    43921 agcgaactgg gtggtgcccg cttctcgttt agctggccgt tatggcataa catcccgcaa
    43981 tttacctctg cttgacatta cgcgcacggt ggtcaagtca ccaccactgt gctataaaat
    44041 aaggatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt agaccgactc
    44101 aacactacct ttcgccagat ggaacaagag ctggctgcat ttgccgctga gctagaacag
    44161 cacaaattgc tggttgcccg cgtgttctct ttgccggagg taaaaaaaga ggatgagcat
    44221 aatccgctta atcgtattga ggtaaagcaa catctcggca acgacgcgca gtcgctggcg
    44281 ttacgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag cagcaagacc
    44341 gctgttcgtc tacctggcgt gttgtgttac caggtcgata acctttcgca agcagcgttg
    44401 gtcagtcata ttcagcacat caatcaactc aagactacgt tcgagcatat cgtcacggtt
    44461 gagtcagaac tccccacagc ggcgcgtttt gaatgggtgc atcgtcattt gccggggctg
    44521 atcaccctta atgcttaccg aacgctcact gttctgcacg atcccgccac tttacgtttt
    44581 ggctgggcca ataaacatat cattaagaat ttgcatcgtg atgaagtcct ggcacagctg
    44641 gaaaaaagcc tgaaatcacc gcgcagtgtc gcaccgtgga cgcgcgagga gtggcaaaga
    44701 aaactggaga gagagtatca ggatattgct accctgccac aggacgcgaa gttaaaaatc
    44761 aaacgtccgg tgaaggtgca gccgattgcc cgcgtctggt acaagggaga tcaaaaacaa
    44821 gtacaacacg cttgccctac accgcttatt gcgctgatta atcgggataa tggcgcgggc
    44881 gtgccggacg ttggggagtt gttaaattac gatgccgaca atgtgcaaca ccgttataaa
    44941 ccacaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt tgcagattaa
    45001 cccccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc aaactcggct
    45061 tcgctaagat accccagcgc aagggccgca gcttttaagg tcagcccttc tttatgcgct
    45121 tttttggcga tctcggcggc tttgtcataa ccaatgtggg tgttaagcgc agtcaccagc
    45181 atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat gcccactgcg
    45241 cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg caggaaatta
    45301 tggatcacca tcggacggaa gacgttcagt tcaaagttac cggaagcgcc acccatgttg
    45361 atcgccacat cgttccccat cacctgacag cagagcatgg ttaatgcttc acactgcgtc
    45421 gggttcactt tccccggcat gattgagcta cccggctcat tttccgggat tgagatttca
    45481 ccaattccgc agcgcgggcc agaggccagc cagcggacat cattggcaat tttcatcagt
    45541 gacgcagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca ggtcgccagc
    45601 gcttcaaatt tgttcggcgc ggtaacaaac ggtgcacaag taatgactgc cagttcatct
    45661 gccacgcgac gcgcatactc cggatgggta tttagtccag tacccaccgc tgtaccgcca
    45721 agagccagtt ccgctacatg cggcaggctg tattcgatat gtttcagatt atgctcgagc
    45781 atcgctaccc agccggaaat ctcctgcccc agcgttaacg gcgtggcgtc ctgcaagtgg
    45841 gtacgaccga ttttgacgat atcagcaaat acgcgggatt tttcactcag cgtctgggtc
    45901 agggttttaa gttgtggaat gagttgcttg cgcagcgcca gtagtgccgc aacgtgcatc
    45961 gccgtcggaa agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt agggtgaact
    46021 ttacgttcca tcccgcgcac gccaccgagt aattcactgg cccggttagc cagcacttcg
    46081 ttcatgttca tgttactttg cgtgccggag ccggtctgcc agatagccag cgggaattcg
    46141 tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct cgctttctct
    46201 tcagacaaca agcctaaatc ttcattaact tttgccgctg cgcgcttggt tagcgccagc
    46261 gcatgaatca gtgaggtggg cattttctcc gtcgaaatgc ggaaatgctc cagcgagcgc
    46321 tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgctcccat cgaatctttt
    46381 tcgctgcgta ctgtattcat ggcctgctcc tcacctgatt aattttttct ttctgttttg
    46441 ctttcgttaa gcaactttta gcttactcat tatttatcat ttgataacaa atgtttggtc
    46501 tttcgtgcca tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgggt
    46561 gcactgtgtg agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa
    46621 tgccgggaaa tcttccactt caattttcca gatggcttcc attcccagtt ccggatattc
    46681 aacacattcc aggctcttaa tgcttccctg cgccaataca gctgccggac caccgatact
    46741 gccaaggtag aagccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc
    46801 tttcgccagc atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat
    46861 ccgtccggcg gtcgttgggc caagagaacc ggaggcataa ccttccggcg ttttggccgg
    46921 acccgcgtag taaatcggat gatctttaat atactgcggc agcccttcgc cgttatccat
    46981 ccgctctttc agtttggcgt gagcaatatc acgaccaacg ataatcgtgc cattcagcga
    47041 taagcgtgta gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt
    47101 aaggtcaacg cgcaccgctt cgccttctcc cgctttacgc agctcttccg ggatatattt
    47161 gcctggatta tgttccagtt tttcgatcca gatcccctga cggttgatct tcgctttgat
    47221 attacggtca gcagagcagg agacgcccat accgaccggg caggatgcgc cgtgacgtgg
    47281 caggcgaatc acgcggatgt cgtgagcgaa gtatttacca ccaaactgcg cacccagacc
    47341 aagattttgc gcttcgatca gcaattcttt ttccagttcc acatcgcgga acgcctgacc
    47401 gtgctcattc ccttccgttg gcagttcgtc atagtattta gcggaagcca gtttcaccgt
    47461 tttaaggttc gtttccgcag aagttccacc aattacgaac gcaatatgat acggaggaca
    47521 ggccgccgta cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt
    47581 tagtaacgct ttggtttcct gatagagata cgtcttgttt gccgaaccac cacctttggc
    47641 gatacagagg aatttgtact cgtcgccatc aacggcataa agatcgatct gcgctggtag
    47701 attggtgccg gtattcacct ctttatacat atccagcggc gcgttttgcg agtagcgcag
    47761 attatcttcg atataagtgt tatagacacc gcgtgccagc gccgcttcat caccgccacc
    47821 ggtccataca cgctgccctt ttttgccaac aataatcgcg gtgccggtat cctgacaggt
    47881 tggcagaacg cctttcgccg cgatgtcgga gttgcgcagg aattgcagcg ccacatattt
    47941 atcattttcg ctggcttccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg
    48001 acgcagcata aatgacgcat catgaaacgc ctgacgcgcc aacaaagtta acgcttcggg
    48061 tgcgactttc aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cgctggttag
    48121 caggtaatac tcagtatcat cttttttgag tggaaaagga gcctgataat gaaagggttt
    48181 gtttgacatt gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt
    48241 tgccctgtta aaagcgagta acaatatcct acacactttt ttaacaaaaa ctgagactag
    48301 tacgactttt tgcggctcca ggttacttcc cgtaggattc ttgctttaat agcgggatta
    48361 atttccacat taaaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat
    48421 gcctggggca gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag
    48481 ccgatggccg agctgtggat gggcgcacat ccaaaaagca gttcacgagt gcagaatgcc
    48541 gccggagata tcgtttcact gcgtgatgtg attgagagta ataaatcgac tctgctcgga
    48601 gaggccgttg ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca
    48661 cagccgctct ccattcaggt tcatccaaac aaacgcaatt ctgaaatcgg ttttgccaaa
    48721 gaaaatgccg caggtattcc gatggatgcc gccgagcgta actataaaga tcctaaccac
    48781 aagccggagc tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgaattt
    48841 tccgagattg tctccctgct ccagccagtc gcaggtgcac atccggcgat tgctcacttt
    48901 ttacaacagc ctgatgccga acgtttaagc gaactgttcg ccagcctgtt gaatatgcag
    48961 ggtgaagaaa aatcccgcgc gctggcgatt ttaaaatcgg ccctcgacag ccagcagggt
    49021 gaaccatggc aaacgattcg tttaatttct gaatgttacc cggaagacag cggcctgttc
    49081 tccccgctgt tgctgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct
    49141 gaaacaccgc acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa ctccgataac
    49201 gtgctgcgtg caggtctgac gcctaaatac attgatattc cggaactggt tgccaacgtg
    49261 aagttcgaag ccaaaccggc taaccagttg ttgacccagc cggtaaaaca aggtgcagaa
    49321 ctggacttcc cgattcctgt ggatgatttt gccttctcgt tgcatgacct tagtgataaa
    49381 gaaaccacca tcagccagca gagtgccgcc attttgttct gcgtcgaagg cgatgcaacg
    49441 ctgtgtaaag gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc
    49501 aacgaatcac cggtgactgt cagaggccac ggccgtttag cgcgtgttta caacaagctg
    49561 taagagctta ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct
    49621 tcaggcggcc agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt
    49681 agcggtaggc gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg
    49741 caagaagatt gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact
    49801 gaccgctcct gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg gcgtattcag
    49861 cagtcagctg caactgttgg tgaaacccat tgccggaaaa gtaaatccgt ggattaaaag
    49921 cggtcagagc gtcatcttca acgaatcggt tgatcatggt cccttcccgc ttgcccagct
    49981 taaaaaactg aatctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataacga
    50041 agtaagcaaa ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcccg
    50101 cattggttac agcggagatt ccagttccga catttcgctc aagccactga attacgagca
    50161 aaaggatgaa aaagtcgcct ttagcggcgg cgagttccag ttaaatgcag acagagacgg
    50221 caaagctatc tccctttccg gggaggcgca aagtggtcgg atagacgctg ttaacgaata
    50281 caaccagaaa gtacagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc
    50341 aagttttggt gagcgcgtag gaaaccaaaa actgtcactg gaaaaaatga ccatttcagt
    50401 ggaaggcaaa gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt
    50461 caatgatggt aaaacgatca atagccaact ggattactcg ctaaacagcc tgaaggtaca
    50521 gaatcaggat ctgggcagcg gcaagctgac tttaaaagtc ggccaaattg atggcgaagc
    50581 ctggcatcag tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga
    50641 aattgccaac aatcccgaac tttatcagga aaaagtgacg gaagccttct ttagcgccct
    50701 gccgctgatg ttgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag
    50761 tcagggtgaa agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga
    50821 agcgccgcaa acgctggcgc aggaagtaga ccgttcggtt aaatctctgg atgcgaaact
    50881 gaccattccg gtggatatgg caactgagtt gatgactcag gtagcgaagc tggaaggtta
    50941 tcaggaagat caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat cagcaatggg
    51001 gcagatgttc cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatgc
    51061 taacggtcag atcacgttaa acgggcagaa aatgccactg gaagattttg ttgggatgtt
    51121 tgcgatgccg gcattaaacg ttccggctgt acccgctatt ccgcagcagt aattcacagc
    51181 caaaatgccg gagatttttc tctccggcat tttttcttac ttcagcataa agtcatactg
    51241 aatactcatt ttcacatccc agtaattcag gcgatcgccc agcgcactgc catcgcgctt
    51301 ttcccgccct ttatcggcag caagaaagaa attcatccct tgtaacggcc cttcgctgac
    51361 ccgatagttc ataccaccta agatacccgc gccttcaatc cgcacatctc gtttactgga
    51421 ttcatagccg cgagtcacaa tcggtgcaaa tgtcagcgta aattgcggcg ttaaacgata
    51481 gttgacaccc aattgccagg actgcattcc ttcacggtta cgatcaagac ttaatgaaaa
    51541 gggatagcca atgtcggtat ccaccacggc accattcatt gcatggcgca aataaccacc
    51601 actgccaaat acgcttcccc acggcgcatt ccaggtcaat tgtccactaa ccaacgccgt
    51661 ttcattgggc tggctggtat tacgacttaa accttcaagt tgcgcgtaaa agccgagcaa
    51721 ctcccctttc aacgtataat tcgggtcaag cggtatgctt ttctcgagaa tactttgtat
    51781 ctgattggaa acatctttct gtaccgatgc ccccagagaa tagcgactgg tatcatcatg
    51841 ccaactggcg gctaaaaaag aacgtgcgcg atgtttatcg tattttcccg tctggctgtt
    51901 atacacccct tcatcacgaa taccggttgt tgaatcggtc cataaaaata ctcgtgaccg
    51961 ccacattccc cacagatcaa tatttggcgt taaagcccag taacctaatg ctccttcaga
    52021 aactgtcggt aatgtacgtt gcgaactgcc agccattaac ggaatacgat aaggtaagct
    52081 accataacta taatccgtcc cgaagcgacc taattttagc aggctatttt cactgggttt
    52141 tatttttact gcccccaggg caaagccaaa acttttatca ccatcaagat accaccgggt
    52201 actcatatca gcacgagcac ctaatttata aacataatac gccccgcctt caacgccaat
    52261 aatattggaa taataaccac tattgaaatc gagcaatcct ccctgtaccc atgcgtaaat
    52321 atctcttccc gtgccagcac tgggcttatc agccctgcgt aattcgtttt tcagtctgat
    52381 gcgtagtccc gcttcatcgt cagccggttg cgccgcgtaa gcagaggtaa ggccagaggc
    52441 agccgtcagg caaataacgg ccatggccac tatttttctc atagttgcac ctttgatgtt
    52501 ctgattattt ttattgaata ttaactagtg atatcgttga ttaattgctg ctgaactttt
    52561 ttacgattat caatttcaac aacgatttct ttgaattttt tatccgtgag cggataaaac
    52621 cagataatga cgaatgccag tagcataaat ccgcaaggta ctaaggcaat tgatgtgcgg
    52681 atgcccataa taacttccgg cgtttgcgcc tgattggcga tatatccgct taatcccaaa
    52741 ataaaggcag gaattgaacc tccgattgcc tgaccgcatt tacgggtaaa tgagaatagt
    52801 gaataggtga gcccttcaat tcgcacgccg gtcaggtatt caccgtattc tacggtatca
    52861 gcttccagtg cccacatcac ggtcatggta acgccctggc caattgatgc gatggccaac
    52921 gcaaccaacg ccaccggcag tgaccagacg gaaacccaga agaacagcag ataaccgcag
    52981 gttcccagca aagcgccaat caggaaggta ttctttttac cgatcctcgc gaccatcccc
    53041 ggcaccagcg gtgccgatgc cacagtaccg accaggtttt gcaccagtac cagcacagtg
    53101 aacagcccgg tatcatttaa cacatagcgc acgtagaaca acgacgaggc gctgaccgcg
    53161 aaggtcgaaa tcagcacaca cagcgcaccg atgcacaaca taaacagcgg gcgattccgt
    53221 ttcagggttt gcagactgat cttcaatgac ggctgcgcca cgatacgtac cacattctca
    53281 cgcgtcgatt tgaagcagat gaagtaaagc accattccgg caatcgccag cacaattgtc
    53341 cagaaatgat ataccgacac catctcttcc gggctggagt tcttaatgct cggtcctatc
    53401 agaaatgcca ggcagacaaa agtcaatgaa gcggcaatcc cgcgagccgc gcccagacgg
    53461 gcgcgggatt gtggttgttg ggtcattgcg gtagcaagtg aaccataagg aatattcacc
    53521 aggctgtagc aaagcccgag gcccatgtaa gtcaaatatg catacactac tttgctgcta
    53581 tggctccagt cggtcggtac ccagaatacc agcacgctga agatcattaa cggcgcagta
    53641 ccgaagagta aaaacgggcg gaattttccc catcgggtat tcacactgtc caccactcgt
    53701 ccggcaaaga catcggcgaa ggcatcgaat acccgcacca gtaacagcat ggtgcccgcc
    53761 gcagcggcac cgacgccagc gacgtcggtg tagtaactca acaggaagag cgcccccatt
    53821 gcgaaggcga agttattggc gacgtcaccg aggctgtagc cgacgatggc gcgccaggag
    53881 agttgttgat tcattgtttg cctccctgct gcggtttttc accgaagttc atgccggtcc
    53941 agcgtttttg cagcagaaaa gccgcagact tcggtttgcg gtcgcgagtg aagatccctt
    54001 tcttgttacc gccaacgcgc aatatgcctt gcgaggtcgc aaaatcggcg aaattccata
    54061 cctgctcacc gacgacagcg ctgacgcgat caaagacgcg gtgatacata tccagccatg
    54121 cacactgata ctcttcactc cacatgtcgg tgtacattga gtgcaggccg gctaacgtat
    54181 ccacaccgta ttcggtgatg ataatcggct gatgcagttt ctcctgccag gccagaagtt
    54241 ctttttccag aaccttctct gccgtttcca aatcgccgct ttggacatac caaccgtaat
    54301 aacggttcag gcacagcaca tcaaagagat cgctgatggt atcggtgtga gcgtcgcaga
    54361 acattacatt gacgcaggtg atcggacgcg tcggatcgag tttacgcgtt gcttccgcca
    54421 gtggcgcgaa atattcccgt gcaccttgcg gacgggtatc cggttcgttg gcaatactcc
    54481 acatcaccac gcttgggtgg tttttgtcac gcgcaatcag ctctttaatc gcctgtaagt
    54541 gcgcctgctg ggtttccccg ttgactgcct cttcgctgta cagttctttc ggcttgttgc
    54601 ccgcttcgaa accaatgcct aaagagaggt taaagccgac agctgcagtt tcatcaatca
    54661 ccacgatgcc atgttcatct gcccagtcga gcatctcttc agcgtaaggg taatgcgagg
    54721 tacggtagga gttggcccca atccagtcca ttagtgcgtg atcgtgcacc atcagcacgt
    54781 tatcgaatcc tttgccgcgc aaatccgcat cttcatgacg accaaagcca gtaaagtaga
    54841 acggtttgtg gttaatcagg aactgttcgt ccttcactgc cactgaccgg atgccgacgc
    54901 gcagcgggta gatatcacac tctgtctggc ttttggctgt gacgcacagt tcatagagat
    54961 aaccttcacc cggttgccag aggtgcggat tcaccacttg caaagtcccg ctggtgcctt
    55021 gtccagttgc aaccacctgc tgatccgcat cacgcagttc aacgctgaca tcaccatttg
    55081 ctaccacctg ccagtcaaca gacgcgtggt tacagtcttg cgcgacatgc gtcaccacgg
    55141 tgatatcgtc cacccaggtg ttcggcgtgg tgtagagcat tacgctgcga tggatcccgg
    55201 catagttaaa gaaatcatgg aagtaagact gctttttctt gccgttttca tcggtaatca
    55261 ccattcccgg cgggatagtc tgccagttca gttcattgtt cacacaaacg gtgatacgta
    55321 cacttttccc ggcaataaca tacggcgtga catcggcttc aaacggcgta tagccaccct
    55381 gatgctccat cacttcctga ttatttaccc acactttgcc gtaatgagtg accgcatcga
    55441 aacgcagtac gatacgctgg cccgcccaac ctttcggtat aaagacttcg cgctgatacc
    55501 agacgttgcc cacataatta cgaatatctg catcggcgaa ctgatcgtta aaacttcccg
    55561 gcacagcaat tgcccggatt tcttgtaaca cgctttccca ccaacgctga tcaattccac
    55621 agttttcgcg atccagactg aatgcccaca gaccgtcgag ttttttgatt tcacgggttg
    55681 gggtttctac aggacgtaac ataagggact cctcattaag ataaaaatac tggtcaacct
    55741 ttaatctgat tagttaacca attctgtgtt gcacacagtt atctgtgaga aacccatgga
    55801 agagaaatgg aaataaaaag aactgacacc gggatcaaaa aaccaaaaac tgcaataccc
    55861 acagcgtgat atagatcgca ttaatcttta aaacggcgtt gacagaagcc ataaaataag
    55921 caaccgaacg ttcgctcgag cataatggga acgataaact ctcatgacat atggctacag
    55981 tgaatatttt ggggggaatt ctccgaaccc gggaaggata atttttgcac agagaaatta
    56041 cgatatgaaa cgcgggcaca gttatcacca gcccgcgaaa tgagaggatc aggatgcggt
    56101 taagataccg ccaatcatcg cccgcaatcc ctgagcaaac gacgcttcac gcgacagatc
    56161 ttccgcttcg atatcggcca gtacaccgta ggtcagatcc agcagtaaac gtgaagtcat
    56221 tgccgtgttg atgtctgggc ttatctcgcc tttttgctgg gcatcacgca tccgctgggc
    56281 aacaaattcc gtgatcgtca tatgcttatt tttaagcatg gcggcgacct gtgggttacg
    56341 catcccttcc gccataattt cgaccaccag cgcccgttgt ccaaaggcct catgggtgag
    56401 agagacaata gactcgacca tatagtcaac gaaatgaatc ccttcaatcg gttcccggaa
    56461 acgggccagc gccctctcct ggtcctgtaa gataatcgcc tgaatcaagg cttctttgga
    56521 gatgaaatgg tgatagagcg tcccgggact aatggcgcaa gatttacaga tggctttcat
    56581 cgaggcactg tgaaatccat tttctgaaaa aatctctctg gcagcattga ggatccgggt
    56641 ccgtgtcggt tgtgcttcag tctgcatgtt atccatcatt aaaccattat agttagcgtg
    56701 aataactagt ttaccgcgtt gtcaggtgag cgcctatcat tgctgttgaa tagtgcgaag
    56761 gcacactcta ttcgtatact tttcactcgg aaattggtta accaatagcc gtaatttggc
    56821 tgccagttat ttagctgaga cgttgatgag tttatcccgg taaacaacac gcacggttgt
    56881 ttaccggtcc gttagtgtat taattgagct cctgtacccc accaccggag acggtgagaa
    56941 tttgtccgct tacccagctg gctgcaggcg agcaaaggaa cagcgccgcg ttagcaatat
    57001 cttgcggttg gcccagacgt ctgatcggcg tgtgctgtaa cattttttgt tcaatttctg
    57061 gtgtaataac ggatttcagg gcatcggtta atattgcccc cggcgcaatg ccattcaccc
    57121 gaatattttt ttcacccagg tcaaacgcca tatttctgac cagatgactg gccgcagctt
    57181 tagatgatgc ataagaagtc atgtttatat ttttattttc tgccgccata gaagtgatgg
    57241 tcagaataac gccaccgcca tttttttcca tttctggcgc aacaagttgt gacagatgga
    57301 aaaaagaaaa cacattcagt tcataagcac ggcgaaaatc tgccattggc atatcaaacg
    57361 gtttaggacc accgccaccg gcgttgttaa ccaggatatc aactttaccc agcttactga
    57421 cggcaaagtc tgccagtgca gagagttcct gttcggaagt aatatcacaa cggcaggcaa
    57481 atgcctgacc acccagttgt tgaatttcat caacaacatg gttagctgcg tcggcgttaa
    57541 tatcactgac caccacagat gcgccagctg tcgcgaatgt aatggcgatt tctttaccaa
    57601 tacctgcacc ggcacctgtg atgatggcac attttccgtc gagtctcagg ttgtcagaat
    57661 taaacacata aacctcctgt gaacttcagt aaaaataaaa ggcaccgcct gtgtgccaca
    57721 ttttttatca ttaaccttga ggaaaggaca ctaccaggta tagcagataa agagaggatt
    57781 atttcgccgc aatgagccgg gcgggaataa taagattgcg tgaatgcgtc tcttcatggg
    57841 tgattttttg catcatgcga tccgcaagtg tggtaccaag ttcccgcgca ggcgtgctgg
    57901 cccaggtaac aggtatatca tcaagtgtgg ttggcgttgc atcggtaaat gccgccaacg
    57961 aaacctgttg ttcaaagtaa cgatcgactc cgctttcccc gctttgccgc cctgctttca
    58021 gcaaaccaaa ccatgccccc atcgcaatag tttcgttata gcaaaccaca gcactgatgg
    58081 tcgggttatg acgtaaaagc gccgtgatag cttccgcggc ttgcttctgg ctggaagagc
    58141 actccaacac ccaatcgctg tgaaacggca ggccaaattt tagtagagtt gcacaatagc
    58201 cccccacccg ttctgcccgg gttaatgagg aactttgccc tcccagccag gcgatacgct
    58261 gatgcccatt gcgaatgaga tgctccgtca acaactgtgc agcctgcatg ttatccgggc
    58321 gaaccgtatc aacatcatca agataactgg cacgggaagc gaaaatcacc gggatagctt
    58381 tttcttctgc catccgtcgc aggtcatcgc tgcttcctgc agccccggca atgaccacac
    58441 catcgacccc ctgattcagt aacagtgaaa accgctgtgc cagctgctcg ccgtctttac
    58501 cgccgtgaag caaaaaaacc atccgtccct gcgcttccag agcttccgtc aatccggccg
    58561 ttaattcggc gtaaaacggc gcagataaat cacggacgat caaaccaatg acgccgcttt
    58621 gcccgccgcg cagcgccgac gcctgacgat tgcgcacaaa tcccagctct tcaatggcgg
    58681 cgttaacgcg ttctcctgtg gcggtagaga ttcgcccttt gccacttagc accagcgaaa
    58741 cggtgcttac cgacacgccc gcagccagcg caacatcatg aatggttatt tttttggcgg
    58801 tagccatgca gatgacctac tccctgatta tgtgacagat aaaacgtttt accttttatt
    58861 tatcttatac ccgctattat cgttgcgtaa tgtgatttat gcctcactaa aatttgataa
    58921 aacgttttat cttctcgcgc aatttactga atccagattg ttctctacga ggagtcgttt
    58981 tatgacggcg aaaacagcac cgaaagtcac gctgtgggag ttcttccagc agttaggcaa
    59041 aaccttcatg ctacctgtgg cattattgtc gttctgcggc attatgctcg gcattggtag
    59101 ttctcttagc agccatgatg tcatcaccct gatcccggtc ctgggcaacc ccgtgttgca
    59161 ggctatcttt acctggatga gtaagattgg ctcgtttgct tttagtttcc tgccagtcat
    59221 gttctgtatc gccatcccgc tgggcctggc acgcgaaaac aaaggtgtag cggcatttgc
    59281 tggctttgtc ggttatgcgg taatgaatct cgcggtaaac ttctggttga ccaataaagg
    59341 cattctgcca accacggacg ccgcggttct gaaagccaat aacattcaga gcattcttgg
    59401 gatccagtcg atcgatacag ggatcctcgg tgcggtgatc gccggtatta tcgtctggat
    59461 gctccatgag cgtttccata atatccgcct gccggatgcg ctggcattct tcggcggtac
    59521 gcgcttcgtg ccaatcatct cctcgctggt gatgggcctt gtcggtctgg tgattccatt
    59581 agtctggcca attttcgcca tgggtattag cggcttaggc catatgatca acagcgcggg
    59641 tgatttcgga ccgatgctgt ttggtaccgg tgaacgtctg ctgttgccgt ttggtctgca
    59701 tcacattctg gtggcattaa ttcgctttac cgacgcaggc ggcacgcagg aagtctgcgg
    59761 tcaaaccgtc agcggcgcac tgaccatctt ccaggcgcaa ttgagttgcc cgaccactca
    59821 cggtttttct gaaagcgcca cgcgtttcct ttcgcaaggt aaaatgcctg cgtttctcgg
    59881 cggcctgcca ggtgcagcgt tagctatgta tcactgcgcg cgcccggaaa atcgccataa
    59941 aattaaaggt ctgcttattt ctggcctgat cgcctgtgtc gttggcggca cgaccgaacc
    60001 gctggaattc ctgttcctgt tcgtagcgcc agttctgtat gtcatccacg cgctgttaac
    60061 cggcctcggc ttcaccgtca tgtctgtgct cggcgtcacc atcggtaata ccgacggcaa
    60121 tatcatcgac ttcgtggtgt tcggtatttt gcatggtctg tcaaccaagt ggtacatggt
    60181 gccagtggtg gcggcaatct ggtttgtcgt ttactacgtc atcttccgtt tcgctatcac
    60241 ccgcttcaat ctgaaaaccc cggggcgcga tagcgaagtt gccagctcaa tcgaaaaagc
    60301 cgttgccggt gcgccgggta aatcaggtta caacgttccg gcaatcctcg aagccttagg
    60361 cggtgccgac aatattgtca gcctcgataa ctgcattacc cgtctgcgtt tgtctgtgaa
    60421 agatatgtcg cttgttaatg tgcaggcact gaaggacaat cgggcaattg gcgtagtaca
    60481 acttaatcaa cataacctgc aggttgttat cgggccacaa gttcagtcag taaaagatga
    60541 aatggccggt ctgatgcata ctgtccaggc ataaggataa gatatgttcg atttttcaaa
    60601 ggtcgtggat cgtcatggca catggtgtac gcagtgggat tatgtcgctg accgtttcgg
    60661 cactgctgac ctgttaccgt tcacgatttc agacatggat tttgcctctg ccccctgcat
    60721 tatcgaggcg ctgaatcagc gcctgatgca cggcgtgttt ggctacagcc gctggaaaaa
    60781 cgatgagttt ctcgcggcta ttgcccactg gttttccacc cagcattaca ccgcaattga
    60841 tacgcagtcg gtggtgtatg gcccttctgt tatctacatg gtctcggaac tgattcgtca
    60901 gtggtctgaa aaaggtgaag gcgtggtgat ccacacaccc gcctatgacg cattttacaa
    60961 ggccattgaa ggtaaccagc gcacagtaat gcccgtcgct ttagagaagc gggctgacgg
    61021 ctggttttgc gatatgggca agctggaagc cgtgttggcg aaaccagaat gtaaaattat
    61081 gctcctgtgt agcccacaga atcctaccgg gaaagtgtgg acgtgcgatg aactggagat
    61141 catggctgac ctgtgcgagc gtcatggtgt gcgggttatt tccgatgaaa tccatatgga
    61201 tatggtttgg ggcgagcagc cgcatattcc ctggagtaat gtggcgcgcg gagactgggc
    61261 attgctaacg tcgggatcga aaagtttcaa tattcccgcc ctgaccggtg cttacgggat
    61321 tatagaaaat agcagcagcc gtgatgccta tttatcggca ctgaaaggac gtgatgggct
    61381 ttcttcccct tcggtactgg cgttaactgc tcatatcgcc gcctatcagc aaggcgcacc
    61441 gtggctggat gccttacgcg tctatctgaa agataacctg acgtatatcg cagataaaat
    61501 gaacgccgcg ttccctgaac tcaactggca gatcccgcaa tccacttatc tggcctggct
    61561 tgatctacgt ccattgaata ttgacgacaa cgcgttgcaa aaagcgctta tcgagcaaga
    61621 gaaggtcgcg atcatgccgg ggtataccta cggtgaagaa ggtcgtggtt ttgtccgtct
    61681 caatgccggc tgcccgcgtt cgaaactgga aaaaggtgtt gctggattaa ttaacgccat
    61741 ccgcgctgtt cgttaacccc aattgcgcaa cgtaaaaaat cgttgcgcaa tcgtggattt
    61801 ttaccctgct ttgtttttat aatggtgcgc acttttatat ccagaaaaag agtgcgacca
    61861 tgattgatac caccctgcca ttaactgata tccatcgcca ccttgatggc aacattcgtc
    61921 cccagaccat tcttgaactt ggccgccagt ataatatctc gcttcctgca caatccctgg
    61981 aaacactgat tccccacgtt caggtcattg ccaacgaacc cgatctggtg agctttctga
    62041 ccaaacttga ctggggcgtt aaagttctcg cctctcttga tgcttgccgc cgcgtggcat
    62101 ttgaaaacat tgaagatgca gcccgtaatg gcctgcacta tgtcgagctg cgtttttcac
    62161 cgggctacat ggcaatggca catcagctgc ctgtagcggg tgttgtcgaa gcggtgatcg
    62221 atggcgtacg tgaaggttgc cgcacctttg gtgtgcaggc gaagcttatc ggcattatga
    62281 gccggacctt cggcgaagcc gcctgtcagc aagagctgga ggccttttta gcccaccgtg
    62341 accagattac cgcacttgat ttagccggtg atgaacttgg tttcccggga agtctgttcc
    62401 tttctcactt caaccgcgcg cgtgatgcgg gctggcatat taccgtccat gcaggcgaag
    62461 ctgccgggcc ggaaagcatc tggcaggcga ttcgtgaact gggggcggaa cgtattggac
    62521 atggcgtaaa agccattgaa gatcgggcgc tgatggattt tctcgccgag cagcaaattg
    62581 gtattgaatc ctgtctgacc tccaatattc agaccagcac cgtggcagag ctggcggcac
    62641 atccgctgaa aacgttcctt gagcatggca ttcgtgccag cattaacact gacgatcccg
    62701 gcgtacaggg agtggatatc attcacgaat ataccgttgc cgcgccggct gctgggttat
    62761 cccgcgagca aatccgccag gcgcagatta atggtctgga aatggctttc ctcagcgcag
    62821 aggaaaaacg cgcactgcga gaaaaagtcg ccgcgaagt
//
LOCUS       DAZGTJ010000018        62476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000018 DAZGTJ010000000
VERSION     DAZGTJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.018"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            186..1337
                     /gene="yiaY"
                     /locus_tag="ACHK8E_001467"
     CDS             186..1337
                     /gene="yiaY"
                     /locus_tag="ACHK8E_001467"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP0343756.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            1467..2762
                     /locus_tag="ACHK8E_001468"
     CDS             1467..2762
                     /locus_tag="ACHK8E_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP0343757.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            2870..4408
                     /gene="aldB"
                     /locus_tag="ACHK8E_001469"
     CDS             2870..4408
                     /gene="aldB"
                     /locus_tag="ACHK8E_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP0343758.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4627..4782)
                     /locus_tag="ACHK8E_001470"
     CDS             complement(4627..4782)
                     /locus_tag="ACHK8E_001470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343759.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRP
                     LTIETRD"
     gene            4953..5276
                     /gene="yiaW"
                     /locus_tag="ACHK8E_001471"
     CDS             4953..5276
                     /gene="yiaW"
                     /locus_tag="ACHK8E_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0343760.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5282..6418
                     /gene="yiaV"
                     /locus_tag="ACHK8E_001472"
     CDS             5282..6418
                     /gene="yiaV"
                     /locus_tag="ACHK8E_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0343761.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6415..7389)
                     /gene="yiaU"
                     /locus_tag="ACHK8E_001473"
     CDS             complement(6415..7389)
                     /gene="yiaU"
                     /locus_tag="ACHK8E_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0343762.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            7513..8253
                     /gene="yiaT"
                     /locus_tag="ACHK8E_001474"
     CDS             7513..8253
                     /gene="yiaT"
                     /locus_tag="ACHK8E_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP0343763.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(8374..10353)
                     /locus_tag="ACHK8E_001475"
     CDS             complement(8374..10353)
                     /locus_tag="ACHK8E_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP0343764.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(10364..11764)
                     /locus_tag="ACHK8E_001476"
     CDS             complement(10364..11764)
                     /locus_tag="ACHK8E_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343765.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            11990..12805
                     /locus_tag="ACHK8E_001477"
     CDS             11990..12805
                     /locus_tag="ACHK8E_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0343766.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(12837..13532)
                     /gene="araD"
                     /locus_tag="ACHK8E_001478"
     CDS             complement(12837..13532)
                     /gene="araD"
                     /locus_tag="ACHK8E_001478"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0343767.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(13526..14386)
                     /gene="sgbU"
                     /locus_tag="ACHK8E_001479"
     CDS             complement(13526..14386)
                     /gene="sgbU"
                     /locus_tag="ACHK8E_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP0343768.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(14379..15041)
                     /gene="ulaD"
                     /locus_tag="ACHK8E_001480"
     CDS             complement(14379..15041)
                     /gene="ulaD"
                     /locus_tag="ACHK8E_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0343769.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(15038..16534)
                     /gene="lyxK"
                     /locus_tag="ACHK8E_001481"
     CDS             complement(15038..16534)
                     /gene="lyxK"
                     /locus_tag="ACHK8E_001481"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP0343770.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(16538..17524)
                     /gene="yiaO"
                     /locus_tag="ACHK8E_001482"
     CDS             complement(16538..17524)
                     /gene="yiaO"
                     /locus_tag="ACHK8E_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP0343771.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(17537..18814)
                     /gene="yiaN"
                     /locus_tag="ACHK8E_001483"
     CDS             complement(17537..18814)
                     /gene="yiaN"
                     /locus_tag="ACHK8E_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP0343772.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(18817..19290)
                     /gene="yiaM"
                     /locus_tag="ACHK8E_001484"
     CDS             complement(18817..19290)
                     /gene="yiaM"
                     /locus_tag="ACHK8E_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP0343773.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(19408..19875)
                     /gene="yiaL"
                     /locus_tag="ACHK8E_001485"
     CDS             complement(19408..19875)
                     /gene="yiaL"
                     /locus_tag="ACHK8E_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP0343774.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            complement(19887..20885)
                     /gene="yiaK"
                     /locus_tag="ACHK8E_001486"
     CDS             complement(19887..20885)
                     /gene="yiaK"
                     /locus_tag="ACHK8E_001486"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP0343775.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21086..21934
                     /gene="yiaJ"
                     /locus_tag="ACHK8E_001487"
     CDS             21086..21934
                     /gene="yiaJ"
                     /locus_tag="ACHK8E_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP0343776.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            22036..22509
                     /gene="ysaA"
                     /locus_tag="ACHK8E_001488"
     CDS             22036..22509
                     /gene="ysaA"
                     /locus_tag="ACHK8E_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0343777.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(22661..23914)
                     /gene="avtA"
                     /locus_tag="ACHK8E_001489"
     CDS             complement(22661..23914)
                     /gene="avtA"
                     /locus_tag="ACHK8E_001489"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP0343778.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(24092..26119)
                     /gene="malS"
                     /locus_tag="ACHK8E_001490"
     CDS             complement(24092..26119)
                     /gene="malS"
                     /locus_tag="ACHK8E_001490"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0343779.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            26439..27263
                     /gene="bax"
                     /locus_tag="ACHK8E_001491"
     CDS             26439..27263
                     /gene="bax"
                     /locus_tag="ACHK8E_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP0343780.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(27371..28549)
                     /gene="xylR"
                     /locus_tag="ACHK8E_001492"
     CDS             complement(27371..28549)
                     /gene="xylR"
                     /locus_tag="ACHK8E_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP0343781.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(28627..29808)
                     /gene="xylH"
                     /locus_tag="ACHK8E_001493"
     CDS             complement(28627..29808)
                     /gene="xylH"
                     /locus_tag="ACHK8E_001493"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP0343782.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(29786..31327)
                     /gene="xylG"
                     /locus_tag="ACHK8E_001494"
     CDS             complement(29786..31327)
                     /gene="xylG"
                     /locus_tag="ACHK8E_001494"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP0343783.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(31405..32397)
                     /gene="xylF"
                     /locus_tag="ACHK8E_001495"
     CDS             complement(31405..32397)
                     /gene="xylF"
                     /locus_tag="ACHK8E_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0343784.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            32763..34085
                     /gene="xylA"
                     /locus_tag="ACHK8E_001496"
     CDS             32763..34085
                     /gene="xylA"
                     /locus_tag="ACHK8E_001496"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP0343785.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            34157..35611
                     /gene="xylB"
                     /locus_tag="ACHK8E_001497"
     CDS             34157..35611
                     /gene="xylB"
                     /locus_tag="ACHK8E_001497"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP0343786.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            35780..36121
                     /gene="yiaB"
                     /locus_tag="ACHK8E_001498"
     CDS             35780..36121
                     /gene="yiaB"
                     /locus_tag="ACHK8E_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP0343787.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            36166..36603
                     /gene="yiaA"
                     /locus_tag="ACHK8E_001499"
     CDS             36166..36603
                     /gene="yiaA"
                     /locus_tag="ACHK8E_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP0343788.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(36645..37640)
                     /gene="wecH"
                     /locus_tag="ACHK8E_001500"
     CDS             complement(36645..37640)
                     /gene="wecH"
                     /locus_tag="ACHK8E_001500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP0343789.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            37814..38122
                     /gene="ysaB"
                     /locus_tag="ACHK8E_001501"
     CDS             37814..38122
                     /gene="ysaB"
                     /locus_tag="ACHK8E_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP0343790.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            38217..39128
                     /gene="glyQ"
                     /locus_tag="ACHK8E_001502"
     CDS             38217..39128
                     /gene="glyQ"
                     /locus_tag="ACHK8E_001502"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP0343791.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            39138..41207
                     /gene="glyS"
                     /locus_tag="ACHK8E_001503"
     CDS             39138..41207
                     /gene="glyS"
                     /locus_tag="ACHK8E_001503"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP0343792.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            41529..41681
                     /gene="hokA"
                     /locus_tag="ACHK8E_001504"
     CDS             41529..41681
                     /gene="hokA"
                     /locus_tag="ACHK8E_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP0343793.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(41869..42081)
                     /gene="cspA"
                     /locus_tag="ACHK8E_001505"
     CDS             complement(41869..42081)
                     /gene="cspA"
                     /locus_tag="ACHK8E_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP0343794.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(42362..42652)
                     /gene="yiaG"
                     /locus_tag="ACHK8E_001506"
     CDS             complement(42362..42652)
                     /gene="yiaG"
                     /locus_tag="ACHK8E_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP0343795.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            43086..43796
                     /gene="yiaF"
                     /locus_tag="ACHK8E_001507"
     CDS             43086..43796
                     /gene="yiaF"
                     /locus_tag="ACHK8E_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP0343796.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(43846..44820)
                     /gene="ghrB"
                     /locus_tag="ACHK8E_001508"
     CDS             complement(43846..44820)
                     /gene="ghrB"
                     /locus_tag="ACHK8E_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP0343797.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(44924..45583)
                     /gene="yiaD"
                     /locus_tag="ACHK8E_001509"
     CDS             complement(44924..45583)
                     /gene="yiaD"
                     /locus_tag="ACHK8E_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP0343798.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            45736..48069
                     /gene="bisC"
                     /locus_tag="ACHK8E_001510"
     CDS             45736..48069
                     /gene="bisC"
                     /locus_tag="ACHK8E_001510"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP0343799.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(48038..48478)
                     /gene="yiaC"
                     /locus_tag="ACHK8E_001511"
     CDS             complement(48038..48478)
                     /gene="yiaC"
                     /locus_tag="ACHK8E_001511"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0343800.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(48475..49038)
                     /gene="tag"
                     /locus_tag="ACHK8E_001512"
     CDS             complement(48475..49038)
                     /gene="tag"
                     /locus_tag="ACHK8E_001512"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP0343801.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            49196..49894
                     /gene="yhjY"
                     /locus_tag="ACHK8E_001513"
     CDS             49196..49894
                     /gene="yhjY"
                     /locus_tag="ACHK8E_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0343802.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            50123..51331
                     /gene="yhjX"
                     /locus_tag="ACHK8E_001514"
     CDS             50123..51331
                     /gene="yhjX"
                     /locus_tag="ACHK8E_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343803.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            51655..53346
                     /gene="eptB"
                     /locus_tag="ACHK8E_001515"
     CDS             51655..53346
                     /gene="eptB"
                     /locus_tag="ACHK8E_001515"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP0343804.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            53438..53514
                     /locus_tag="ACHK8E_001516"
     tRNA            53438..53514
                     /locus_tag="ACHK8E_001516"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53472..53474,aa:Pro,seq:cgg)
     gene            53549..53682
                     /locus_tag="ACHK8E_001517"
     ncRNA           53549..53682
                     /ncRNA_class="other"
                     /locus_tag="ACHK8E_001517"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            54427..56034
                     /gene="dppA"
                     /locus_tag="ACHK8E_001518"
     CDS             54427..56034
                     /gene="dppA"
                     /locus_tag="ACHK8E_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP0343805.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(56097..56324)
                     /locus_tag="ACHK8E_001519"
     CDS             complement(56097..56324)
                     /locus_tag="ACHK8E_001519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343806.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            56342..57361
                     /gene="dppB"
                     /locus_tag="ACHK8E_001520"
     CDS             56342..57361
                     /gene="dppB"
                     /locus_tag="ACHK8E_001520"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP0343807.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            57371..58273
                     /gene="dppC"
                     /locus_tag="ACHK8E_001521"
     CDS             57371..58273
                     /gene="dppC"
                     /locus_tag="ACHK8E_001521"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP0343808.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            58284..59267
                     /gene="dppD"
                     /locus_tag="ACHK8E_001522"
     CDS             58284..59267
                     /gene="dppD"
                     /locus_tag="ACHK8E_001522"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP0343809.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            59264..60268
                     /gene="dppF"
                     /locus_tag="ACHK8E_001523"
     CDS             59264..60268
                     /gene="dppF"
                     /locus_tag="ACHK8E_001523"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP0343810.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(60298..61569)
                     /gene="yhjV"
                     /locus_tag="ACHK8E_001524"
     CDS             complement(60298..61569)
                     /gene="yhjV"
                     /locus_tag="ACHK8E_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP0343811.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            62045..62152
                     /gene="ldrD"
                     /locus_tag="ACHK8E_001525"
     CDS             62045..62152
                     /gene="ldrD"
                     /locus_tag="ACHK8E_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0343812.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
BASE COUNT        15275 a        15499 c        16259 g        15443 t
ORIGIN      
        1 tgattaattc ataataaata tgaattaata caaaatttct tattaatgtt aagaaggcac
       61 gttatttgat gaatgtaacg tgctttttat taaaaatatt ttaataaacg gtaatgttga
      121 tcatattttc aaatgaaaat cctcagtaag ctgcccgccc ttttttacac tttcaggagt
      181 gtgttatggc agcttcaacc ttttttattc cttccgtgaa tgttattggt gctgattcat
      241 tgaatgatgc aatgaatatg atggcagatt atggatttac ccgtacctta attgtcaccg
      301 acaacatgtt aacgaaattg ggtatggcgg gtgatgtgca aaaagcactg gaagaacgca
      361 atatttttag cgttatttat gatggcaccc aacctaaccc caccacggaa aacgtcgccg
      421 aaggtttaaa attacttaaa gaaaataatt gcgatagcgt gatctcctta ggcggtggtt
      481 ctccgcacga ctgcgcaaaa ggtattgcgc tggtggcagc caatggcggc gatattcgcg
      541 attacgaagg cgttgaccgc tctgcaaaac cgcagctgcc gatgatcgcc atcaatacca
      601 cggcgggtac ggcctctgaa atgacccgtt tctgcatcat cactgacgaa gcgcgtcata
      661 tcaaaatggc gattgttgat aaacatgtaa ctccgttgct ttctgtcaat gactcatctc
      721 tgatggtcgg tatgccgaag tcactgaccg ccgcaacggg tatggatgcc ttaacgcacg
      781 ctatcgaagc gtatgtttct attgccgcca cgccgatcac tgacgcttgc gcactgaaag
      841 ccgtgaccat gattgccgaa aacctgccgt tagccgttga agatggcagc aatgctaaag
      901 cgcgtgaagc aatggcttat gcccagttcc tcgctggcat ggcattcaac aacgcatctc
      961 ttggttatgt tcatgcgatg gcgcaccaac tgggcggttt ctacaacctg ccacatggtg
     1021 tatgtaatgc cgttctgctg ccacacgttc aggtgttcaa cagcaaagtc gctgctgcgc
     1081 gtctgcgtga ctgtgctgct gccatgggcg ttaacgtgac aggcaaaaat gacgcggaag
     1141 gcgcagaagc gtgcatcaat gccatccgtg aactggcgaa gaaagtgaat atcccggcag
     1201 gcctgcgtga cctgaacgtg aaagaagaag atattccagt gctggcaacc aatgccctga
     1261 aagatgcgtg tgggtttact aacccgattc aggcaactca cgaagaaatt atggcgattt
     1321 atcgcgcagc gatgtaatca tcattcccac aatggctgtc taattgtcag ccatttttta
     1381 aactatctca aaatttgcga tttttgagat agtttctctt tcctgctata cactatctca
     1441 atattgcgct tttttgagat aagcacatgg ctatcaaatc acctcccgga cttattcctt
     1501 tatcacactt atcgggtgag gaactcctgg cccacctgcg ctttaaccgg gttaccgatg
     1561 aaaaaggacg ttatctgcct tttgatgagc tgcagtaccg catcaaaaaa ggagaaaacg
     1621 tcgatgtggc gtggacgctt acccgtctgg cgcgcaacgc ggcgatccaa cgaattaatt
     1681 attgcaatga agcgggtgaa caggccggat tcaacattac gccggtgatt gcagaagcgt
     1741 gcgagcttgt tgataaacat gccactgcac tggcgctgaa agatcagact gaacggttac
     1801 gcggggcggg cgccgagtta agtcagttac gcctggaaga gccgatcacc agttcccaac
     1861 tggaaggtgc gaatactaca acgctggtgg cgagaaaaat gctggaaaca gggcggtcgc
     1921 cacgcactga agacgagcat atgattgcgg gaaatgcccg gctgatggcg gaaattccgc
     1981 acttactcgc agaaccgctg acacctgcgc ttattcgcca gttacatgcc attggtatgg
     2041 gtggaatcaa tgacgcgaaa tatcgtccgg gagaatttcg tgaaacagat gatgttgtga
     2101 tcgccgacta tgatggcaat atagtgcatc aaccgcccgc tgcggcattg ttaccggaac
     2161 ggctggaaaa agtttgccag tggttaaaca gccatgaagg gtatattcat ccgttggtcc
     2221 gtgcctgcat tctgcatttt atgctggccc atgaacatcc gtttcgcgat ggcaacgggc
     2281 gcaccagtcg ggcgctgttt tactggtata tgctgaaatc gggctacgat gtctttaaat
     2341 atatttccat cagccgtttg cttcatgctg caccggtcaa atatgccgcc agctaccagt
     2401 acactgaatc agatggtatg gatctgactt atttccttga atatcaggcg ggtgtcatta
     2461 aacgtgctct gcaaaactgg cagcagcata tcgatgaaat tacacaacga agcgcaaaac
     2521 tcgatagtgt gctgtttagc tctggtgtgc ttaaacgcct gaatccgcgc caggtaacgc
     2581 tcctgaatgt gatgctcgcc aatccgggga aagagtacac agttgctgaa attagcgcga
     2641 gcttgggcgt ctctgataat acggctcgag ctgatttacg gacaattgtt aaagaaggat
     2701 tcgcgcaaga gaaaagaatt aataaccagc aagccgtgta cgtcgcacac tacccgctgt
     2761 agcttttgcc cgtaaattcg tgatagccgt cgtaaagctg ttaccgactg gcgaagattt
     2821 cgccagtcac gtctaccctt gttatacctc acaccgcaag gagacgatca tgaccaataa
     2881 tcccccttca gcacagatta agcccggcga gtatggtttc cccctcaaat taaaagcccg
     2941 ctacgacaac tttatcggtg gcgaatgggt agcgcctgcc gatggcgagt attaccagaa
     3001 cctgacgccg gtgaccgggc agctgctgtg cgaagtggcg tcttcgggca aacgagacat
     3061 cgatctggcg ctggatgctg cgcacaaagt gaaagataaa tgggcgcaca cctcggtgca
     3121 ggatcgtgcg gcgattctgt ttaagattgc cgatcgaatg gaacaaaacc ttgaactgtt
     3181 agcgaccgct gaaacctggg ataacggcaa acccattcgc gaaaccagtg ctgctgatgt
     3241 gccgctggcg attgatcatt ttcgctattt tgcctcgtgt attcgggcgc aggaaggtgg
     3301 gatcagtgaa gttgatagcg aaaccgtggc ctatcatttc catgagccgt taggcgtggt
     3361 ggggcagatt attccgtgga acttcccgct gctgatggcg agctggaaaa tggctcccgc
     3421 gctggcggcg ggcaactgtg tggtgctgaa acccgcacgt cttaccccgc tttctgtact
     3481 gctgctaatg gaaatcgtcg gtgatttact gccgccgggc gtggtgaacg tggtcaacgg
     3541 cgcaggtggg gaaattggcg aatatctggc gacctcgaaa cgcatcgcca aagtggcgtt
     3601 taccggctca acggaagtgg gccaacaaat tatgcaatac gccacgcaaa acattattcc
     3661 ggtgacgctg gagctgggtg gcaaatcgcc aaatatcttc tttgctgacg taatggatga
     3721 agaagatgcc tttttcgata aagcgctgga aggctttgca ctgtttgcct ttaaccaggg
     3781 cgaagtttgc acctgtccaa gccgtgcttt agtgcaggag tctatctacg aacgctttat
     3841 ggaacgcgcc atccgccgtg tcgaaagcat tcgcagcggt aacccgctcg acagcgtgac
     3901 gcaaatgggg gcgcaggttt ctcacgggca actggaaacc atcctcaact acattgatat
     3961 cggtaaaaaa gagggcgcag acgtgctcac tggcgggcgg cgcaagctgc tggaaggtga
     4021 actgaaagac ggctactacc tcgaaccgac gattttgttt ggtcagaaca atatgcgcgt
     4081 gttccaggag gagatttttg gcccggtgct ggcggtgacc accttcaaaa cgatggaaga
     4141 agcgctggag ctggcgaacg atacgcaata tggcctgggc gcgggcgtct ggagccgcaa
     4201 cggtaatctt gcctataaga tggggcgcgg catacaggca gggcgcgtgt ggaccaattg
     4261 ttatcacgct tacccggcac atgcggcgtt tggtggctac aaacagtcgg gcatcggacg
     4321 cgaaacccac aagatgatgc ttgagcatta ccagcaaacc aagtgcctgc tggtgagcta
     4381 ctcggataaa ccgttggggc tgttctgata taagaagctg gtcgcattgg gtattcattg
     4441 cctgatgcga cgcttacgcg tcttatcatg cctacgggga cctgaccgta ggccggataa
     4501 ggcgtacacg ccgcatccgg cataaacaaa gcgcacgttg ttacaattta cgttttaaac
     4561 ctacgtgcgc aataacccag actacgtcaa cagaagtgcg tttctggggc gtgttttgcc
     4621 aggagtttaa tctcgcgttt cgatggtgag cgggcgaacc aggccataaa tcaccattgg
     4681 cgttccgagg ccaatcagca ataaacggta gataattgaa ggtttaatac gcttaatctg
     4741 tactgtggat gttttactgg agttattcat tagtttggcc aatagttcca ttatttcaat
     4801 atgataagcc tcgcactgtt gaaaagccag gctgcacact tcagaaagta tataaaaaac
     4861 aatatgtcgt tatataaata aggaaattgc accgcattat ggtatttcat aatatgcgtt
     4921 aattgttctt ttctatattc tctgagggaa atatgttcct ggattatttt gcattgggag
     4981 tgcttatttt tgtatttctg gtgatattct atgggattat tattttacat gatgttccct
     5041 acctgattgc caaaaaacgt aatcatcctc atgccgacgc cattcatgtt gctggttggg
     5101 tgagtctttt tacgttacat gttatctggc catttttgtg gatttgggcc acgctttacc
     5161 gcccggagcg tggatgggga atgcaaaacc atgactcctc cgttgtgcaa ctacaacagc
     5221 gtattgccgg gctggagaaa cagctcgctg acatcaaatc ctcttctgcc gagtaatact
     5281 tatggattta ctgattattt taacctatgt ggctttcgca tgggcaatgt ttaagatctt
     5341 caaaattcct gtaaataaat ggaccattcc cacagcggcc ctgggaggca tatttattgt
     5401 cagcggtcta attctgttaa tgaactataa ccatccgtat acctttaaag cgcaaaaagc
     5461 ggttatttct attcctgttg tcccacaggt gacaggcgtg gtgatcgaag tgacggacaa
     5521 gaaaaatacg ctgattaaaa aaggtgaggt gctatttcga ctggacccga cgcgttatca
     5581 ggctcgggtg gatcggctga tggcggatat cgttaccgca gaacataaac agcgggcgtt
     5641 gggcgcagag ttagatgaga tggcggcgaa tactcagcag gcaaaggcca cgcgggataa
     5701 gttcgctaaa gagtatcagc gttacgcacg tggcagccag gcgaaagtaa acccgttttc
     5761 agaacgcgat atcgatgtgg cgcggcaaaa ttatctggcg caggaagcct ccgtaaaatc
     5821 agcggcggcg gaacaaaaac agatccagag ccagctggat agcctggtgt tgggtgaaca
     5881 ttctcaaatc gccagcctga aagcgcagct cgcggaagca aaatataacc ttgagcagac
     5941 gatagtgcgt gcgccgagcg atggttatgt gacccaggtg ctgattcgtc cgggtaccta
     6001 tgccgcgtcg ctgccgctac gtccggtgat ggtgtttata cccgatcaga aacgacaaat
     6061 cgtggcgcag ttccgtcaga actccttgct gcgcctggct cccggcgatg atgcggaagt
     6121 ggtatttaac gctctgccag gcaaagtgtt cagcggtaag ctggcagcca ttagtccagc
     6181 tgttcccggt ggagcttatc agtcgaccgg caccttacag acgttaaaca cagcgccggg
     6241 ttcagatggc gttatcgcga ccattgaact ggatgagcat actgatttga gcgcattacc
     6301 agacggtatt tacgcccagg tggcggtcta ttctgatcat ttcagccatg tctcggtgat
     6361 gcgcaaagta ctgttacgca tgaccagctg ggtgcattac ctttatctcg atcattaatc
     6421 ttcgctttcc gccgcttgtt caggccgcag ttgctgcgac tgagcaagct ggtggcgaat
     6481 ttcacgcagc atttcatcca gcacatactg gacccgccag ggctggtatt cccgtttacg
     6541 gaagatagcc accagatcca gccaccattc atacgggtgc tgaaagcagg gcaccagcgt
     6601 gccgtcttgc aggtcgctct gcacactttt gtctggcgca aacacaatcc ccaggtgatt
     6661 tcgtgccagc tccagcgcag attgcgtgtt gtcgcaaacg taatttccct tcacgcgata
     6721 atcccgcacc tctttactcc cggcgacgtt gaagcgccag atattcgcgt cgtcgatcat
     6781 catcgagtcg atcaaaatac aggagtgatg ttcgagttcg tcaggacggc taatgggatg
     6841 tttctcaaga tagcgctggc tggcataagc ggttacggca tattgggtaa taaaactggc
     6901 aaccagcgat tcatcttttg gttgggcgta gctgattaaa acatcacaat catcgggaaa
     6961 ttcgacgcct tcataaaagg cattacgctc aagattgcag gtttttagcg ataaggtgat
     7021 atcaccgata tctttaattt tatcgattac atgtttggat aaataggtaa ttatgccggt
     7081 tggtgcatag atagtgaccc gaccacgttt ctcatgctta taatctgcaa taaaattatt
     7141 aagctgctcg tttctgtcca gcatgtcgtt gatatacggt aacagcacgg tgccaaaagg
     7201 tgtgagcatc agctgtcggg tggttcggtc aaagactttt aaacccactt ttgattcaaa
     7261 atcagcaaga tatttgctga cgttggcctg tgcgatgcca agtacagtcg cggcatggct
     7321 gatattttca ctggcagcga ttaccgagat aatttttaac tcccggtact taagttgtaa
     7381 ttttgtcatc gcaacagccc aattatatat aattttatat attactatat aaatatgaat
     7441 cttgcaccgg agaaattgta agcattacta tgccgactct tttttatctt attttaaaat
     7501 ggaataccgg acatgttaat taaccgcaat attgtggcgt tatttgcgtt gccttttatg
     7561 gcaagcgcaa ctgcttctga attatccatt ggtgctggtg cggcttataa tgaatcgcct
     7621 tatcgcggtt ataatgaaaa tacaaaggca attccgctga ttagttatga aggtgatact
     7681 ttttatgttc gtcagaccac gttaggtttt attctgtcgc aaagtgaaaa aaatgaactt
     7741 agcctgaccg catcctggat gccgctggaa tttgacccta ccgataatga cgattatgcc
     7801 atgcaacagc ttgataagcg tgatagtacg gctatggcgg gggttgcctg gtatcaccac
     7861 gagcgttggg gaaccgtgaa agcctctgcg gctgcggacg ttctggataa cagcaacggc
     7921 tgggtggggg agctatcggt attccacaaa atgcagatag gtcgcctgtc gctgacacct
     7981 gcgctgggcg ttctctatta tgacgagaat ttcagtgact attactatgg catttcagag
     8041 agtgagtccc gtcgtagcgg tctggcaagt tattccgcgc aggatgcctg ggtgccttat
     8101 gtcagcctga cggcaaaata cccgatagga gagcacgtcg tattgatggc gagcgccgga
     8161 tacagcgagc tgccggaaga gattaccgac agcccgatga ttgatcgtaa tgagagtttt
     8221 acctttgtca ccggcgtgag ctggcgtttt taatttaccg gtggatgtcg gtgcggctgg
     8281 cctacgctct cgcaccgact cgtgggcctg ataagacacg ttaagcgtcg catcaggcaa
     8341 tgtgctccct acgttggatg cggcgcgagc gccttatcca acctccggat ggcaatgctt
     8401 ttcctcattc acccagatcc gcatttcgcc ttcaccccgg ttggcccagc taaaccacgg
     8461 gataaaggtc agggtttgcg gctggcgttt ggctggcgcg ctgtcgtaat gccacagcgg
     8521 ttgctgctct ggattgctct gttcataccg ataacccggt gcctggatta agatcttatg
     8581 gcggaacaat cccttacctt caaatgtcgt aaatggcgca tcggcgggca gccacagatt
     8641 atgcagtgac tcgccgttgt cggcctgttc caggcaatac accagcgggc cgcgctgaat
     8701 cgccactttt ccggcgacgt gacgcaccag agggttaccg taaacgcgac gtaccggcat
     8761 tggcaaagtc agattcagcg tgtcgccctc ctgccattcg cgggtaatgt gcaaataccc
     8821 tttacgaata tcctgctcga cctcttcccc attcaatatg atctgcggct gtgtgcacca
     8881 gtccggcaga cgtaaagcca gcgtatgacg caccggctgg ggcgattcaa ccgcaattgt
     8941 cacctgttcc tgccacggat agtttccgct aacccgcaga cgcagcgtgc cattttctac
     9001 cggcacttcc atgctgtttc ctgcgtagat gttgatatac aacgcatctt cacgcggcgt
     9061 gtagagataa tgaccaatcg aggttagcac gcgggcgata tttggcggac aacaagcgca
     9121 accaaaccaa cgctggcgga tcggcttaac gtgatcgtag atatggttga attttaacgt
     9181 ttttggatgc acttccagcg gattaacgta gaagaaatgt ttgccatcca gcgccatgcc
     9241 gccgagcaca gtgttgtaca gtgcgcgctc catcacatcg gcatattgac tgtcgccttc
     9301 catttccagc attcgccggg cgaacatcat caggccgatg gaagcacaac tttcggcgta
     9361 aaccgtgtca ttcggcagat cgtaatcgct gctgaacgct tcgccgctgc tttgtgagcc
     9421 aatgccgccg gtaatatata actgacgctg ggccatattg ttccacagcc gcaggcaatc
     9481 ctgacgcttg ctttcatcgt gacttaaacg cgcgagatgt gcgacgccag tcatcagata
     9541 tacaaagcgt actgtatgtc cgatggcggt ttgttgctgt gcgatgggca aatgcgcctg
     9601 gctgtaggct ttatctttca ccatccacgc cgggccgtag gtgtgccagt gcgaggtctg
     9661 cccgcgcttt tcatattcct ggtcgtaata gtgcggttgc gcaccacgct gttcgacaaa
     9721 atagttcgtc agcgccaggt agcgcggctc ttcggtcact tcatacaggc gcattagtgc
     9781 cagttcaatt tccgggtgac caggataacc gtgtaactta ctttcacctg gaccaaatac
     9841 gctgtcgata tgatcggcca gacggcaaac cacttccagc aagcgccgtt ttcccgtggc
     9901 ctggaagaag gcgactccgg cttcaatcag atgaccggcg cagtaaagtt catgacactc
     9961 cgccagattg ctccagcgtt cttcgggcgc ttttaccgta aagtaagcat tgagatagcc
    10021 atcttcgcac tgggcggagg cgatcagttc aatcacctcg tcagcggttt tttccagttc
    10081 ggcatccggc ttctggcaca gcgaccaggc taccgcttcc agccatttgg cgacgtcgct
    10141 gtcctgaaac accatcccgt aaaattcacc gtcttgcagt cctgcggcaa tgcgaaagtt
    10201 ttcaatcgca tggctgggtt ccgcttctgg gatacgatcg ttcaaggcgt cccactgata
    10261 aggaatcacc acatcgcgga ccagttgttg gtactgaccg aggaacggat cgctgaccgt
    10321 cagtttatgc agatcgactt ccgaaatgtt catcttgtgc tccttaaggc tgtacatgac
    10381 gctgacgcag gtcgctagca atgcgtgaca tcatagggtt attgagtttg caaaagcgga
    10441 tcgcccctgc cagcagcagg tggaatagcg caggcagtaa tgtttccatc gccgtgatcc
    10501 cgccaagcga actggtggtc tggttttcca cgcctggctg gtaggcgaca aaaataaaca
    10561 gcaggctgat gatcccggcg ctggaggccc atgccagctt gatgaaaaac agattgaagg
    10621 cgaagttcat gccggaagaa cgtacgcggg ttttccactc gccgtagtca tcggcaaagg
    10681 ccatcaatga gaagtgcagt ggcaatgtga agccaagaat cacgccatta ccgaggatca
    10741 ccgccagcca cagcgtttga taagccgggc cagaaggcag gaaccacatc aacaccgcca
    10801 gcgcagcgag gagcaggttg gtgtagtagt aaattttgac ggtatcgaaa cgccgcgtta
    10861 acgggctgac aatcaccgag ccgataatgg aggcgaaggt gaccatggtg aagaacagcg
    10921 acgtgtagcc cgtactgcct tgtaagacgt aggtaatgaa gtacatatac ccaccgccgc
    10981 gaatattaaa gacgttaatc agcaggaaag acatgaccag catcagcagc agttggtcgt
    11041 tgttccgcag cccggcaaga tgttcgcgca gggtaaattt ccccattgtg gagagtggca
    11101 cccgttcacg aacccagaag aaacagcaca ggaacatcac cacggcaatg gcgcacagca
    11161 cgccgacgcc cagttgatag ccgcgcgcgg cattaccctg accgaagaga tccaccatcc
    11221 acggcaggcc aaccgaaacc agaaatcccg ctacgccgca gagaacgaat cgccaggact
    11281 ggcaggagat cacttcattg tggcgggtgg tcatggtgtt gatcagcgcg cagtacggta
    11341 cgttgatggc ggtataaccg actgaaagga gcaggtaagt accgaacgcc caggcgattt
    11401 ttactcccat gctggcatct ggcacggtga acgtcaggat gccgataatg ccgatgggaa
    11461 tggcaatcca taactgccac ggacgaaagc gcccccagcg actttgcgtc cggtcggcaa
    11521 tgacgcccat caccgggtcg gagatggcat caaacacgcg cagtgcaata aacagcgtgc
    11581 cgaccagcgc cggagttaag ccaaaaacgt cggtgtagaa aaaggtgaga aagttcatga
    11641 tcaggcaagt tattacggtg ccgcctgcgt cacccaggcc gtagcctatt ttttcccgta
    11701 cagataacgc atcgtcaatt ttacctttag cgatatcagc ggcagtaatc ggtgtagagg
    11761 tcatagatat tcctcgtgaa tagggagcaa tttttagttt tattgcaatg acctgattaa
    11821 ttctgccgtg aacgggaata tcaacaaggc gagaaaagag taaaaaaagg tgacgattcc
    11881 gacctgaatt taaaagtgtg aaatggatca ggtgaaatta atgtttttga ttaataatca
    11941 aaaggtaatg tgtttaatca ttaatgaata agcgggaaat tacatatcaa tgctcgaatt
    12001 atccatgacg ttaccgatta aagtgcaaaa tggtggatta tttatttccc gtggcgtagg
    12061 gaggcacccg gcaaggcggc tggagtcatg ggaaattatc tttgtcgaaa aaggtcgatt
    12121 aacgattcag gaagaagagc ggatatttct ggtcgatgcg ggagagagtt tattactctg
    12181 gccgaaccga cagcatgttg gggtagagga ttttcccgcc gatctcaaat tttactggct
    12241 gcattttgag gttaaagcgc cggatagcga cccgcgctgg ctcactcatc ttagcgtgcc
    12301 gcaacaaacg aaggttgccg atccgcaggc gctggtggcc ctgtttcgcc agttcttaag
    12361 cgaacaggaa aagcatcaac gcactccggc gctggagttt attgtgctgc tgattttgca
    12421 gcagctcacc cgtgacgcgc gtcttgatga acaggccgat gccgcaggta ccacgcttgc
    12481 ctggaaagcg cagaaactga ttcgcacgca atatcatcgc ccgctttcta cggcgctttt
    12541 agcccgcgag ctgtactgca atgccgatta tcttgggcgc gtctaccggc gggtgtttca
    12601 tctgacgtta acagaagcaa ttcatcgcca gcgagtgcgc gaagccgaaa agctgttaat
    12661 tagcgaagcg cgatcgctca ctgaagtggc tgcgttgtgc gggtttaatg acgttggcta
    12721 tttccgacag atttttcgca aacacaccgg tttaacgcca gctgcgtgga agcgacgtta
    12781 cagcaaggaa catatcaatt cgtagtgccg gggcaatgaa gccccggcgt gagggattac
    12841 tgcccgtaat aggcattggc cccatgctta cgcaggtagt gcttatctaa cagttcgttt
    12901 tgcatcgcag ggagctgcgg cgcaagctgg cgcgagaaca ggcccatata ggcgcactct
    12961 tccagcacta cggcattatg caccgcatcg gcggcatttt ttccccaggc gaacgggccg
    13021 tgagaatgca ccagcaccgc cgggatttgt gccggactcc tgccgcgttc ttcgaaagtt
    13081 tcaatgatca cttcgccggt ctgatattca tattcgccgt taatctcctc tgcggtcatc
    13141 tgtcgcgtgc aggggatggc accatagaaa taatcggcgt gcgtggtgcc ccaggcgggg
    13201 agatccagcc cggcctgcga ccagatggtg gcgtggcgcg agtgggtatg cacaatgcca
    13261 ccgatttcgg catagcgacg gtaaagcgcc agatgcgttg gcgtgtcgga cgagggcttt
    13321 ttgctgcctt ccaccacctt accgctggct atctcaacca ccaccatatc gtcggcggtc
    13381 atcacgtcgt actcgacgcc ggaaggtttg attaccatcc attgccgcgc ttcgtctacc
    13441 gcgctgacat tgccccaggt gaacgtcacc agatggtgag cgggaagtgc cagatttgcc
    13501 gccagcacgt cggctttcag ttgctctaac atataaaacc tgcctcctgc atacgcgctt
    13561 caatccagcg ccgcgcctga ataatctcca gcaccggctc tttggctttt tcggtccaca
    13621 tctcaatcag gaaagaaccg cgatagttca gttcatgcag cgttttgaag atgccaacga
    13681 aatcgacgca gccttcgcca aacggcacat cacggaactg tccggggctt tgctcggtga
    13741 ctggttgagt atctttcagg tggatcgcgg cgatacggtc aatgcccagt ttcagttcgg
    13801 cgggaacatc attgccccag gcgctgaggt tgccgacgtc cgggtagacg gtgaaccacg
    13861 gcgaggcgag catctcgtcc cactttttcc atttgctgat ggagttcata aacgcagtat
    13921 ccataatctc caccgccagc attacttgtg atgccgccgc ctgttcgact gtccacgcca
    13981 gcccttcagc aaaacgttgc cgggtgcctt cgtcgtggtc ttcgtaatag acgtcgtagc
    14041 ccgccagctg gatggtgcga atgccgagat cgcgcgccag gcgaatcgct ttactcatga
    14101 tttcccgcgc ccgttcgcgc actgcctcgt cacggctacc aaaaggaaag cgacgatgtg
    14161 cggacaggca catcgacggg atcccaacac ccgtttcgat catcgcagtg accagcgaag
    14221 cacgttgtgc ggcgctccag tcaagacgtg agagccgttc gtcggtttca tccaccgaca
    14281 tttcgacaaa atcaaagcca cagctttttg ccagcaccag ccgctccggc caggagagat
    14341 ctttcgccag cgctttttca taaatcccta acggatgatt acgcacgcgc acctccccag
    14401 atggcatcaa tttgtgcatg gaaatcgcca gccacctgca ccggatttgc tgcgcctgcc
    14461 agtgctcgcc cggcaataaa cgctttcacg cgaatatctt taaacagcgg caggtcagca
    14521 ggggtaatcc cgccggtaat ggaaagctca aggccgatat cggaaagcgc cttcatgcgt
    14581 gccagatcgg cctcgcccca ctgttgcccg ctggcctgcg cgtcgcgacc gcgatgataa
    14641 atggcctgcc gcacgccaat acgatgccag tcgcgggcgt cgtccagcgt ccagttaccg
    14701 aacaactcga tctgaatttc accaccgcag cgttgcgcca tcgcgtggcc tttttccacc
    14761 gtcgcgagcg gtgcggcgca gatgatggtc atccagttag cgcctgcgcc aaacgcctgt
    14821 tgcgcgagcg tttcaccggc atcggcgacc ttccagtcag cgacgatgat tttgttcggg
    14881 cactgttcgc gcaaggcttt cactgcgcca agcccttcgt ttaaacagag aatggtgccc
    14941 gcttcgacga tatcgacgct gtcttttaac agcgtcacgt cgcgctgtgc gtcttcaagt
    15001 gatgagtggt cgagggccag ttgcagaagt ggtcggctca taatgtgtgc tccttgatgc
    15061 gggcgtgaaa gccctgaagt gcggcaatga gatgctgata acgttggtat ttttgttggt
    15121 aaagctgatg ggcggtcata tccggcagca gggtgcgtac cgggtgctgt aaatcacgtt
    15181 gggcttcgct gaaatcgcga taaactccgg tgccgacgcg ggcggcaagt gccgcaccaa
    15241 agcagccggt ttcttccacc tgcggcagct cgatacgcag accgctgacg tccgccagca
    15301 tttgcatcca gacatcggag tgcgccggac cgccagtgac gcgcagggtg tgcacatcag
    15361 taaaacgttc gcgcatccgg ttgagatggg tcatatggct gaacaccacg ccttcataaa
    15421 tggcctgcaa caggtgtacg cgggtgtgaa tggcctgcat cccgtagaaa ccgctggtca
    15481 tctcgagtcc agcgttgctg ccgtacagga acggcaggaa aaagagatcg cccccggcct
    15541 tcggcaagct ggcaacggcc tggttgatct catcaaacga gatttctccc cactgtgtgg
    15601 taaaccattc gagattgccg gaagaggtgg ggcttgcttc atgaacgata aattgaccat
    15661 cgttaacgta gcgaccatag acatacggat gcgcttcacc gtcacgtaaa ccacgggtta
    15721 tgccgctggt caccgcccag gtgcccatca ccgcattgag ggtaaattcg tcttcgatcc
    15781 cggcgcagag tgcggtggaa accacatcaa acaggccgcc gacaacgggc gtacccgctt
    15841 tcagaccggt cagtgcggct gtctgagcgg tgatctcccc gcagatttcg gcagatccga
    15901 caacaagtgg cagggcgtgg ttgatttcag cgatccccag ccagtcggtg aggcacgggt
    15961 catattcccc aagactcatg ttgtagagat tggactcaga aatattgctc tcttcgcaac
    16021 ctttgacgcc ggttaaacac cagcgcaggt agtcgtgcgt catcatcacg cagccaattt
    16081 gcgcgtagcg ttccggttcg tgctctttca gccagcgtaa cagcgacacc ggatgcccgg
    16141 tccacaaggt ttgtcgcgtc agcgggtaga gtttttccgg gatgccatct tcctgccagc
    16201 gacgaacgat ttccatcgcc cggcgatccg aggacaaaat ggcgttaccg agcggcttgt
    16261 cgtttttatc cagcaaaaac aagccctttc cctgtgcgga gatgccgata ccgacaattt
    16321 gttccccgct aacaccagaa tgagtaagca gggcgcgaat gacagccatg cagcattgcc
    16381 acagttctgc catatcgcgc tctgcccaac ctggctgcgg gcttaatgcg cacagcggca
    16441 ggcgctgcac gcctgcctcc cggccttcgc ggtcatacag cccggctttc agccagctac
    16501 cgccacaatc taaccccagc cagtattgcg tcatggttta ttgcacctca tccacggctt
    16561 tcagcagctc agcgccctgc ggaacatctt taacaaacat gtcgcggacc tgagtaccca
    16621 gtgcgtcgct aaaggctttg cggtcgagat cggtgatgac ttccacgccc gcttctttca
    16681 tgccgtcgat gattttttgc tgatcttcgg caaccagttt gcgctggtag ttacccgctt
    16741 cctgtgcaga tgaaactagc gcctgctgga actccgggct taagccatca aacttcgctt
    16801 tgttgatcac caccagaagt ggcgaatagg cgtggtgcgt cagagaaagg tacttctgta
    16861 cttcgaaaaa ttttgctgac cagacgacgt tgatcgggtg ttcctgagcg tcgatagtgc
    16921 gcgtttccag cccggtatag acttcggcaa acggcatcgg gatcgggtta gcgccaaaga
    16981 ctttgaatgc ggcgatattc atcgggctat tgttggtgcg aattttcagc cctttcaggt
    17041 cggcgggggt tttaaccggt gcgcgcgagt tggtgacatc gcgccagccg ttttcccagt
    17101 aggccagtac ttttagtcct ttaccttcaa gtgaggcttt cagatcatca ccgactttgc
    17161 cgtcgagcgt tttatgcgcg tgagcggtat cgcggaacag gaaagggaca tcaagcaagt
    17221 tcatcactgg tgataacccg gcaaagttat tcgagccgga catttccata tcgatggtgc
    17281 cgccacgtac gccgctgatc atcgcctgtg cgttaccgag agtactgtcc gggaacagtt
    17341 ttaatttcag ctcgcctttg gttctctcct gcaataaatc attgaatttt ttcgccgcaa
    17401 tatgttgcga gtcggtttgt gaggtttcat aaccgaaacg taaagattgc gccgccagtg
    17461 aagatgtact aaatgcagcc aggccagcaa tgaataatgc gtaggttaca gagcgtaatt
    17521 tcataatatt ttccttttaa ttaatccatt tcaaagggag gatgatgaga tcgggaataa
    17581 aaacaaatac gactaataat gaatataaaa ccaggacgta agggaaaacg cctctgactg
    17641 catcatcgaa tttgagtttt gccacccctg aaataacgtt aagcacatta ccgataggcg
    17701 gtgtaattaa gccgattgag cagttaatga taaacatgac accgaaataa atcggatcga
    17761 ttcctgcctc tttaactaaa ggcattaata ccggggtaag aattaatacc gtcggcgtta
    17821 aatccatgac catgccgaca atcaggatag ccaccataat gacgataaac agcagacgcg
    17881 gtgaatcgac cagcggttgc agtaaatcag aaaccatcat cggcagttca gcaatggtga
    17941 tcagccaggc ggaaacttgt gccgaggcta ccagaaacat caccactgag gtcgttttcg
    18001 ctgcgccaat gagcacatgc caaagtgtgg cgaaggtcat ttcacggtaa ataactgtgg
    18061 cgacaaacag cgcatagaaa gcagcaacgg ccccggcttc ggtaggggta aacagaccgg
    18121 agcgaaaccc gccaataatg atgactggaa gaaacagcgc ccagataccg gagacaaaag
    18181 agtgccagat ttcctgcatc gttgcttttt gctggcgggg taaattaagg cgactggcct
    18241 gccaccacca ggtaagcatc agcgttgcgc ccatcatcat gccaggggca atgcccgcca
    18301 taaacagctt actgatggat aatccgctgg aaacgccgaa aataataaac ggaatggaag
    18361 ggggaataat tggcgcgata atgccgccag aggcaatcag ccccgcagcc cggttgaccg
    18421 ggtagttagc gctgcgcatc atcggcacta atagcgcggc aacggcggcg gtatcagcaa
    18481 cagcagaacc agaaaggctc gccataatca tagccgccag cacgccgacg tagcccaacc
    18541 cgccaggttt atgccccacc agtttcatcg gcaggtcaac aatgcgtttt gacaagccgc
    18601 ccgcattcat gatttcaccc gccagcacaa agaacggaat cgccagcagg gagaagctgt
    18661 cggctccgtt caccagcgtt tgtgccatga tctggacatc aaacatgtcc agccagaaca
    18721 ttaacgccgc tccgcacaac aacagtgccc aggcaatagg caaaccgata gcaataccac
    18781 ccaacagaca gcccagaaaa atcagcacag ccatgattaa gctccttgcg gtggcgacgt
    18841 tagagaattg ctacgcgtga tgagttgata taaatgacgc agttcaaaga atgcgataac
    18901 gaggctggtg ggcagacaag cggcatacat caggccgatg ggcaaaccga ggatcggtga
    18961 ataatcgctc cagtcctgaa ttgtttttag cgttgcgccc caggccagtg cgccacaaat
    19021 aaataagatt aaggaatggg taaccagagc gactcgtcgc tgccatgcgg gggagagttt
    19081 ctccaccaga aaggtgacct gaacgtgggc gttatccata aaagccacaa tcgcgccaat
    19141 aaatgttaac cagacaaata aataacgtga caattcatct acagataaaa tgcttgtctg
    19201 aaaaccatat cttaaaataa tgtttataaa cacaatacag gaaagtacgg cgagattaat
    19261 cgccagtatt gcttcaagta tttttttcat agctattcct tgaggctatg tctgtcataa
    19321 ttcaagttgc atgtgcagca acctaaatta ttaatatagt tagagcaata acattaccca
    19381 ggcgttctgg cgtagacgat tattcgatta attcagcgct gttaatgcga ctttaaccac
    19441 aatttttcgt atctcagagg ttgtttgcag aatacaacct ggtcggtgaa catcctgcgg
    19501 gaagaaaata gcgtagctgc ccggtatcat ttctataaat gattcatgtt cactatcgtg
    19561 ataaaaaata atatcgcgct gctctaatag tgattcgctg actttattat ttcccgtatc
    19621 aatagcaatg ccgattttct cttcacccca cgccagaaac tgaatatcga tataccgacg
    19681 atgcacttcc ggacggtttt ccgccgcttc gcgtgtggtt aaatcgataa tttgcgcata
    19741 aatattcttg ccgtcgattt cgacaacgcc cggttccagg gcgttgaagt cggtggtgcg
    19801 cagaaaatca agcgcctttt caatggcggc aggcaaacgg cacgggtcag gctgcgcgat
    19861 atgtccaaaa atcatgggtt atctccttat aacgcttgga tttttgccca cacgctgtca
    19921 tcaacggtaa taccgttacg gcggttttcg gccagcaggg tagtaaattc gtggccgggt
    19981 aagcgaatgg cctgattttc gtcagcgcgt tcagcggtgg taacgtaatc catgatgcgt
    20041 tgcagtttgg catcgcgggt ggggccgtcg ataagcttat ctacttcaat ggcaataaaa
    20101 atttgtgaaa tgccgtattc gtcgctgttg tcctgggtga cttcggcaac cgatgcgccg
    20161 tcagaaagga gagtggcgat catatccagc acaatcgaca tgcccgaacc tttccagtaa
    20221 cccatcggca aaatgcggcg attcttctcg ataacgccag gttctttggt caaattgccc
    20281 tcatcatcaa agccaccatc gaccgggagc tgacggcctg ccagacggtt aacttctaac
    20341 atgccgtaag agaacatcga catcgacata tcgaccatgg tgattggggt agaaggaatg
    20401 gcgacgatca gcgggttagt gccgatgcga cactcttttg cgccccacgg cggcattacg
    20461 gcgatggagt tggtccagca aatgccaata tagccttttt ccgccgcctg ccagccgtag
    20521 ctgccgccgc gcatccagtg gttggcatta cgtagtgcca ccagaccaat accgtgatcg
    20581 gcagccagtt caatggcgcg atccatcatc tttttcgctg tcaggttacc gatcgaacgc
    20641 tgggcgtccc actgttcaat tgcgccgagg ctggttatac gtttgggttg ggcatcagga
    20701 atgatatcgc cgttttccag ttgttgaatg aaacgaggga aacggttgac gccgtgagaa
    20761 taaacgccgg attcggtggt gcgggcgaac atttcggcac aggcatcagc cgtttcgctg
    20821 tcaacgtcgc gtgaaattaa gactcgatta aaggctgctt ttaactgctc aaatgtcact
    20881 ttcatccctg gcttccttgt ttttttgatc gcttttttgg cttaaaattt caatatgcga
    20941 aatctgattt caaatatatc gatcgctttt taacaaagca atctgattga tgaatttcaa
    21001 aaaacttaaa aatcaagtgg ttataaatta tctgtcgaga tcgtgaacta cgacacactt
    21061 tgcgctacca tcttgacgcg acaaaatggg gaaagaagtg atgggaaaaa aagagaacga
    21121 gatggcgcag gaaaaagagc gtccagccgg aagccagagt ctgtttcgcg ggttgatgct
    21181 gattgagatt ttgagcaact atccaaacgg ttgtccgttg gcgcatcttt cggagctggc
    21241 tggtttaaat aagagtaccg tccatcgctt attgcaggga ttacaatcct gcggctatgt
    21301 gaccaccgcg cccgccgcag ggagttatcg cctgaccacc aaatttattg ccgtcgggca
    21361 gaaggcgctg tcttcgctga atatcattca tatcgccgct ccgcatcttg aggcactgaa
    21421 catcgccact ggtgaaacta ttaacttctc cagccgcgaa gacgatcacg ctattttgat
    21481 ttataagctg gaacccacaa ccgggatgct gcgaacccgt gcctatattg gccagcatat
    21541 gccgctctac tgttccgcaa tgggcaagat ctacatggcg tttggccacc cggactacgt
    21601 gaagtcatac tgggaaagcc atcagcatga gatccagccg ttaacccgca ataccattac
    21661 cgagctgccc gcgatgttcg acgaactggc gcacattcgt gaaagcggag cggcgatgga
    21721 cagagaagaa aacgaactcg gcgtctcctg tattgctgtt ccggtgtttg atattcatgg
    21781 gcgggtgccg tacgccgtgt cgatttcgct ttcgacatca cgtctgaaac aggtggggga
    21841 gaaaaatctc ctgaaaccac tgcgtgaaac cgcgcaggct atttctaatg aactgggatt
    21901 tactgtcagg gacgaccagg gcgcgatcac ataacgcttt ggacaagtgc caaaacttta
    21961 acatttcctt cgttggatca aagcagtaga gacgcgctct ctggcactct gctgttttag
    22021 tgcaaaggag tgatcatgaa ccggtttatt attgcggatg cgacgaaatg tatcggttgc
    22081 cgtacctgtg aagtggcttg cgcagtgtcg catcaggaga atcaggattg cgctgcgttg
    22141 tcaccagacg agtttatttc ccgtattcgt gtcattaaag accacacatg gaccacggct
    22201 gtagcctgcc atcagtgtga agatgcaccg tgcgcgaatg tctgccctgt tgacgcgata
    22261 agccgcgaac atgggcatat tttcgttgaa caatcacgtt gcattggctg taaaagctgt
    22321 atgctggctt gcccgtttgg tgcgatggag gtcgtttctt cgcgcaaaaa ggcgagggcg
    22381 attaagtgcg atctgtgctg gcatcgggcg acgggaccgg cctgtgttga agcctgcccg
    22441 acaaaggcgt tgcagtgcat ggatgtcgag aaagtgcagc gacatcggct acggcagcag
    22501 cctgtttgaa acgtttttcc ggcgtttctc aacgcaggga tagtgcgctt tgtttatgcc
    22561 ggatgcggct aaaacgcctt atccggccta caaattctac caaattcaat atattgcaga
    22621 aatcatgtag gcctgataag cgtagcgcat caggcaattt ttagtgactt tcagcccagg
    22681 ctctttctat ctcttctgcc agaatcttca cacccgcctc aattttctcc ggctccggca
    22741 cgtagttcat gcgcatacat tgatgtgtat gtggccacgg tttatccagc cctgggaaga
    22801 agttgtgccc cggcaccatc agcacgcccc gtgctttcag gcgctggtag agctgctcgg
    22861 tcgtaatggg caaatcctta aaccatagcc agaggaaaat ggctccttcc ggtttatgaa
    22921 tcaggcagcg atcttccggt aaatagcggc gaatgatagc gatagtttcc tgaacacgct
    22981 ggtagtaaaa cggtttgatg actgtttcag acaggcgcag cagatcgtta cgcttaatca
    23041 tttcactcat catcgccgga ccaataccgc caggtgccag gctgataatg ccgttcatat
    23101 tggtgatggc ggtgatgatt ttttcattgg cgatgataat gccgcagcgg gagcctggta
    23161 gacccagctt ggaaagactc atgcacagta cgatattcgg attccacagc gggcgcgctt
    23221 cgctgaagat gatacccggg aacgggacgc cataagcgtt atcaatcacc agcggaatgc
    23281 cgtgttgatt cgccagcgcg tcaagcttca gcaactcttc gtcagtaatc acattgcctg
    23341 ttgggttcgt cggccgggag acgcaaatca tcccggtttc ttcgccaata tgcagatgct
    23401 caaaatcgac gtggtattta aactggcctt ccggcagcag ctcaatattc ggacgcgcag
    23461 agacaaacag atcttcttcc agtccggcgt cagcgtagcc aatgtattcc ggtgccagcg
    23521 gaaacagcac ttttttgatc cgaccatcgg cacggcgtcc ggcgaacaga ttaaataagt
    23581 agaaaaacgc gctctggctg ccgtttgtta gtgcaatatt ctgtggttcg atatcccaac
    23641 ccaacttctc gcgtagcatt ccggcaagca gtgtgagtag ctccgttttc ccctgtggac
    23701 cgtcgtaatt gcacagtgca tcagtcgctt tgccactttc cagcatgtcg gtcagtagcg
    23761 tctggaagta gtcctgcatt tccgggatct gcgctggatt accgccgccg agcataatcg
    23821 cgccaggcgt gcgtaaaccg tcgttcagat cttccatcag cagcgtaatg ccggagtggc
    23881 gggtaaattt gtcaccaaaa agggagaatg tcatagcggg atatctgtcg aactctgtag
    23941 caaggaaggt aacaataacg ctacatttgc tggggtgcaa atcgctctgt tgaagaggga
    24001 gcggtgcttt atcctgcaac gctgattagg tctgacattt tatcctggtt ggcggcttcc
    24061 cgaggtactg acgaacggga agccggaaaa gttactgttg cccagcccag atgaccagca
    24121 ctttatcgtc gccatgctca cgaacaaagc cgtagccctg cttcagcgaa agtgtcgttt
    24181 gtttgcccgc gccaattgcg ggatggcggg cgcggaactg gctgattttc tgccagtgcg
    24241 cgacgctggc ggcagatttg ccgctaacat cctgccagtt catatccgaa cgcgtgcctt
    24301 gcagtggatc cgaaccggtg gggccgaacg gacgcgagga ttcatcacca taaaagattt
    24361 gtaccgcgcc tggcgctaat aataactctg ctgctttgtc gcccccttca cggaacaggc
    24421 gggtatcatg cgacgagagg tagctcaaca cgttgaaatc ctgcaatttc tccgccattt
    24481 gctgccaggt cgtatccatc tgcgccagac aatcgactgc tttagccgcc tgctcctgat
    24541 aatcgaaatt gatcatcgca tcgaagccgt ggcgatagta gtcactttgc atcacgccgt
    24601 ggccccaggc ttcaccggtc atccagaacg gtttgtcatc taacgcttta tcgggattcg
    24661 cttttttcca ttcgacaagc gcggcgctgg cttcggtttt cagttgctgc caggcgggca
    24721 actcaacatg tttggcggtg tcgacccgaa aaccatcaat cccatagtcg cgaacccact
    24781 gacttaacca gtgggttaag taatcgcgag gggtaaagcc gtcgatgact ttagcgtggg
    24841 tatccgtttt gtttttatag aacaccggca gaccagaagc ggtcgttgat tcggttttga
    24901 tatccggcaa aaaggccagc gacatagtga gatcgtcaaa tccaggattg tcgtaatcgc
    24961 cgatatcggt tctgatccag ttttttcccc accatttatc ccagcctgtt ttgtcgctga
    25021 aattaatgta atcgttaaag ctatgccagg tttgcccggc ggcaggtttc cagtcgctcc
    25081 agcgttcacc caacgttttt ttcacttcgt caccagaaag atataacgcg ccaaactgat
    25141 actcctgcat atccgccagt gtggcatagc cggtgtggtt catcacgaca tcaaagagaa
    25201 tacgaatacc gcgctgatgc gcgctatcaa ccagtgtccg tagatcggct tcactgccca
    25261 tattggcatc aagattcgtc cagtcctgtg tgtaataacc gtggtaggca taatgcggga
    25321 aatcgccttt tgtaccgccg ccgacccagc cgtgaatttg ctcaaatggg gcacttatcc
    25381 ataaagcatt gacgcccagc tgctggaggt aatccagttt gttggtcagg ccgcgtaaat
    25441 cgccgccgtg aaaagtgcca atttctgcca taccgtcttt atgacgtccg taactctggt
    25501 cattactggg atcgccgttt tcgaaacgat ctgtcagcac aaagtaaacc gtggcgttat
    25561 gccagtcgaa aggggcaggg gcgtcagttt ctgcccgttc cagcaggagt aaaccgttgc
    25621 tggtggcagc gggttgtaac gttatttgac cgttcttcac tatcgcaatt tgctggctgt
    25681 aataatcccg gacggcggct ccctccggaa aagtggcgct gacatccagt gtgagcggta
    25741 atccatccca tttcgggcat tcacggacca ggcttgctac cggttcgacg gcgttctgga
    25801 tggaaatcat caatgttggt gtaccggagc gggtgtctag ttgcagcgta tattcgccgt
    25861 ccctgaacaa tcgccattga ggcggcgtgt tgctacaagg ttgcagggaa agcatctgat
    25921 tgagttttat cgcatccgca ggctgccagc actgttggtc aaaatttagc gttagtggac
    25981 gcgtaccttt gggcaactgc gcgtggctga caaatgttcc cgtcccctgt tcgctaaagg
    26041 tgggaaaccc cggagaagtc cagctggcgg caacggcgaa gccaggaagg agtgtcagaa
    26101 aacaggcggc gagtttcatg gcggatgagt ccttattgct gcgatttcag acagtttgcc
    26161 agcgataagc cagacaaaac tcatccctga gcggtaagtc cacaggatga gaagcaaggg
    26221 tgagcgatcg cgcacaaaaa acggctgaat tttgcgataa accccacatt ttctgcgatt
    26281 tagcgccaat ctgaatcgtt aactcgtgac atagtttcag atttggacta ttccttgggg
    26341 tgctcttgac agctattttt gatatgattt gagattcaac tctcaaattt gtgaaaaata
    26401 taaggtgttg gaatgattaa atccgaccag gagacctgat gattttgact cccatacgac
    26461 gatatggggc gatgattctt atgttactca ctctggtgtt ttcgagtgag gtgttagcga
    26521 agacgcacac aacaacagcg agtcaaaagt cccacttaac taaagctagt aataaacagg
    26581 taagcagtaa acaagagtat tctcgcaata gtgcaaagag tagttcactt cctgatttgc
    26641 gaaaataccc ttccggaaca ccaaggaaaa aggcgtttct ccggactgta atgccttaca
    26701 ttaccagcca aaatgcggcc attactgcgg aacgtaactg gctgatttca aaacagtatc
    26761 agggccaatg gtcacctgct gagcgtgcgc gtctgaaaga catcgccaaa cgctacaagg
    26821 tgaagtggtc tggtaatacg cgaaaaatcc cgtggaatac cttgcttgaa cgcgtagaca
    26881 ttatccctac cagtatggtg gcgacgatgg ctgcagcaga aagcggttgg ggaacgtcaa
    26941 agctggcgcg caacaacaac aatctgttcg gcatgaaatg catgaaagga cgttgtacca
    27001 atgcgccggg taaagtgaaa gggtactcac agtttagttc tgtcaaagaa tcggtgagcg
    27061 cctatgtcac taacctgaat actcatccgg cttactcttc gttccgtaaa tcgcgtgcac
    27121 agctgcgtaa agcggatcag gaagtgactg ccacagcgat gattcacaag ctgaagggct
    27181 actcgactaa agggaagagt tataacaact acctgttcgc aatgtaccag gataaccaac
    27241 ggttaatcgc ggcgcatatg taattgcatt tcctcgagcc ttatccgact tgtcagtcgg
    27301 ataaggcttt tgcttgcatc aggtggttgt gctgagttac ctgatgcgac gccgacaatt
    27361 ctcatcatcg ctacaacatg acctcgctat ttacatcgcg atactctttt ggcgtcgtgt
    27421 catatgcttt tttaaaaaca gagtagaaat attgcagcga tggataaccg cacatttgcg
    27481 atatctcatt gatcgacaag gtggttgaaa tcagcagact gcgcgctttc tccagcttct
    27541 cggcatgaat catggcatgg atggtttcac ccacctcttc tttaaaacgc ttctcaagat
    27601 tggagcgcga gatcccgacc gcatccagta cctgatccac tttaatccct ttacaggcgt
    27661 gattacgaat gtaatgcatg gcctgaataa cggcgggatc ggtcagcgag cggtaatctg
    27721 ttgagcgccg ttcaatgacg cgaactggcg ggaccaaaat ccgctgtagc ggcatttctt
    27781 ctttatctaa taatcgatgc aacagttttg ccgcctgata gcccatttgc cgcgcgccct
    27841 gagcgaccga agaaagggcg acacgcgaca gatagcgggt cagttcttcg ttatcgatgc
    27901 caatcacgca taatttttcc ggtacgggaa tatgtagatg ttcacatact tgcagaatat
    27961 gccgcgcccg ggcgtcagta acggcaataa tcccggtttg cggtggcagc gtttgtagcc
    28021 agtctgccag ccgattttgc gcgtgttgcc agttctctgg cgcggtttct aacccctgat
    28081 aaaccactcc gcgatacttt tcttcggcga caagctgacg aaatgcatat tcgcgttcag
    28141 tggcccaacg tttaccgctt gattccggaa gaccataaaa agcaaagcgg ttaacgcctt
    28201 tctcttttaa atgcaaaaat gcgctttcaa ccagcgcata gttatcggtg gcaatgtaat
    28261 gaacgggtgg gtaactttcg gcaaggtgat acgagccacc aaccccaaca atggggacgt
    28321 cgacatcagc cagcgcttgc tcgatttgtt tgtcgtcgaa gtcggcaatg acgccatctc
    28381 ctaaccagtc cttgatttta tcaatgcggg cgcggaaatc ttcttcaatg aaaatatccc
    28441 attccgattg tgacgcctgt aaatattccc ctacgccttc tactacctgc cggtcatagg
    28501 ctttattggc attgaacagt aatgtgatgc ggtgacgttt agtaaacatg gttcttttcc
    28561 tgctgaatca tgcaaaaact caaaaccggt aatacgttac cggctttgag aaaattttta
    28621 tcaaaatcaa gaacggcgtt tggttgcgga gtccatccat acagccagca acagaatcgc
    28681 acctttaacg atatactgcc agaaggtcgg tacatccatc atactcatgc cgttatccag
    28741 tgaagccatg atgaatgccc ccattaccgc tccagccacg ctcccgacgc cgcctgccag
    28801 actggtgccg ccaatcacac atgctgcaat tgcgtccagc tcggcgatat tccccgcaga
    28861 aggtgaacca gcgccaagtc gagaactgag gattaatccg gcgatggcta ccattaatcc
    28921 gttaatcgcg aacacggcaa gtttggtgcg ttcaacgtta atcccggaga gacgtgccgc
    28981 ttccagattg ccgccgatgg cataaatgcg tcgtccaaat gctgtccgcg ttgccataaa
    29041 cattccgccg agtaacagca acgtcagcag cagaacagga gtgggaacgc cacggtaatc
    29101 attcaacagc cagattgcgc ctaatacgat gatagcggtt aaagcctggc gaccaactac
    29161 tgcggtagag gcgggagatt gcaaacccaa agcctgacgg cgcattcttc cgcgccattg
    29221 ccagccaaca aaagccatta agccaagcgc gccaatgatg aagccagtgc tggcaggtag
    29281 atagctttgc ccaatttgtg acatcgcggc gctggtgggg gatacggtcg tgccgttggt
    29341 gatgccaatg agtatgccgc gaaatgccaa catgcccgcg agggtgacaa taaatgaagg
    29401 gactttgcgg tacgcgaccc accatccgtt ccaggcaccg agaagcaatc ccagaaccag
    29461 cgttacaatg atggtaagtg gcaaaggcca gcctagccag acgtcacaaa tcgccgcgac
    29521 gccacctaac agccccatca ttgagccgac ggaaaggtcg atttcagcag aaattatgac
    29581 gaacaccatt cctaccgcga ggatgccggt aatcgcggtt tggcgtaaca ggttggagac
    29641 gttacgggcg cttaagtagg caccatcggt ggtccaggta aagaacagca tgattgcgat
    29701 gatagctgca atcatcacga agacctgcaa attcagtgat ttcagcccgg aaaagccacc
    29761 ggatgtcggt acggctaatt tcacttcaga cggattgctt ttcgacatga tgttcgctcc
    29821 tcaatgcggc ttccatcacc tgctcctgag tcaggttatg atttatcagg ttggctttta
    29881 gtttcccttc atgcatcacc agtacacgat cgctaaggcc gagcacttca ggtaattcgg
    29941 aagagatgac aataacggca ataccctgct ggacgagttg gttaattaat ttgtagatct
    30001 cgtatttcgc gccaatatcg atacccctgg tgggttcatc aagaatgaga atgcgcgggt
    30061 taagtaacag acagcgagcg aggatcgctt tttgctgatt gccgccgctc aaacgtccaa
    30121 tagcaaggtc gggggacgac gttttaactt tgagttgctg gattgattcc agaatacatt
    30181 tttgctctgc cgcgtcatca agctggctaa tgccaccggt aaattttttg agtgcggcga
    30241 gggtaatatt tttaccaacc gccattaccg gaacgatgcc gtcgcgcttt ctgtcttcgg
    30301 gtaccatcgc aatcccctgg gcgatggctt gctgacagtt acgaatatct acctgtttgc
    30361 catcaatata aattttccct tcccattgtc cgggccacac accaaacagg cactgaatag
    30421 tctcggtacg tccggcacca acgagtccgg cgatacccag tatttcgcca cgtttcaggg
    30481 aaaacgagac atcattaact cgtttaatat gacgattaac cggatgccat gccgtcagat
    30541 gttcaatacg taatatttca tctccggtgg tatgtggttc attagggtaa agcgcggtta
    30601 actctcgtcc gaccatcatg gtgataatat cgtcttcact cattccagca gcatcacgcg
    30661 tgccaatgtg ctgaccgtcg cgaataacgc aaatcgtatc ggaaatcgct ttgacttcgt
    30721 tgagtttgtg cgaaatataa atacaggcga taccgtgctg ttgtagatcg cgaataatat
    30781 ccagtaaaac cgacgtttcc tgctcagtta atgaggctgt cggctcatcg agaattaaca
    30841 aacgcacctg tttattaagt gccttggcaa tttcaaccag ttgttgttgc ccaagtccta
    30901 aatcgccaac gcgggtatca ggtgaaatgg ataaactgac ctgtgcgagc agcttctgac
    30961 agcgtagcgt catcagatca taatccataa tgccattatg ggttatttcg ttacccagga
    31021 agatattttc cagcacggtc aattctttca ccagggccaa ttcctggtga atgatggcga
    31081 tacctttgcg ttcggtatcg cggatgtgac tcgcctgaat ctcttctccc gcaaaaataa
    31141 tttcgccttc gtaggaacca tggggataaa taccacacag cactttcatc agcgttgatt
    31201 taccagaccc attttcccca cagagtgaga cgatttcgcc agcattcaac cgcaagctga
    31261 cgttatcaat cgccttcaca ctgccgaagg ttttggtaat gttcttcatt tcaagtagat
    31321 aaggcataac gactccacct aagccaatta attcacacgg catggagaga aatcacgccc
    31381 ccgctcctcg ccggggcgta acgcttacag ctcgctctct ttgtggaatc cgtctttaac
    31441 taccgtgtct ttgatgttgt ttttattcac atcgatcggt gtcagcaggc gggagggaac
    31501 atctttcagg ccattattca gtgtggtatc tgcttttggt tcctgaccat tgcccaactc
    31561 aacggcaatt tctgcggcag tatttgccaa caacgtaata ggtttataca ccgtcatagt
    31621 ttgcgtaccg gcagcaatac gtttaatacc tgcgagatcc gcatcctggc cggaaattgc
    31681 tactttccct gataaacctt gcgcgcttaa tgcctgaatt gccccacctg cggtggcatc
    31741 gtttgaggca actacagcat caattttgtt attattggcg gttagcgcgt tttccataat
    31801 tttcaatgcg ttttccggta accagccatc aacccattgg tcaccaacga ctttaatttt
    31861 tccagaatca acgtaaggtt ttaacacttt catttgtccg gcgcggaaca gcttagcgtt
    31921 gttatctacc ggcgagccgc ccatcaggaa gtaattacct tgcggaacaa tatcgaccag
    31981 ggcttttgcc tgcagttcac cgactttttc gttatcgaaa gaaatataaa aatcgatatc
    32041 cgcatcgtta atcatacggt cgtaagctaa tactttaata ccttcttgtt tggcttcttt
    32101 tacaacgtta cttaatacct gaccgttata aggaataatg acaagaacat cgacaccccg
    32161 gttaatcatg ttttcaatct gcgacatttg tgtttcttca ttgccatttg cagactgtac
    32221 aaatactttc gcgccgagag attctgcttt tttcacaaaa atatctcgat ctttttgcca
    32281 gcgttcaaga cggagatcat caatcgccat acctattttg acttctttgg cgtgtgccgc
    32341 aacgttggta agcagaagtg aggtgcaaag ggtgagtaga atgttcttta ttttcatggt
    32401 gtagggcctt ctgtagttag aggacagctt taataagtaa caatcaccgc gataaacgta
    32461 accaattttt agcaactaaa caggggaaaa caattacaga tttttatctt tcgattacga
    32521 tttttggttt atttcttgat ttatgaccga gatcttactt ttgttgcgca attgtactta
    32581 ttgcattttt ctcttcgagg aattacccag tttcatcatc ccattttatt ttgcgagcga
    32641 gcgcacactt gtgaattatc tcaatagcag tgtgaaataa cataattgag caactgaaag
    32701 ggagtgccca atattacgac atcatccatc acccgcggca ttacctgatt atggagttca
    32761 atatgcaagc ctattttgac cagctcgatc gcgttcgtta tgaaggctca aaatcctcaa
    32821 acccgttagc attccgtcac tacaatcccg acgaactggt gttgggcaag cgtatggaag
    32881 agcacttgcg ttttgccgcc tgctactggc acaccttctg ctggaacggg gcggatatgt
    32941 ttggtgtggg agcgtttaat cgtccgtggc agcagcctgg tgaggcactg gcgttggcga
    33001 agcgtaaagc agatgtcgca tttgagtttt tccacaagtt acatgtgcca ttttattgct
    33061 tccacgatgt ggatgtttcc cctgagggcg cgtcgttaaa agagtacatc aataattttg
    33121 cgcaaatggt tgatgtcctg gcaggcaagc aagaagagag cggcgtgaag ctgctgtggg
    33181 gaactgctaa ctgctttaca aaccctcgct atggcgcggg tgcggcgacg aacccagatc
    33241 ctgaagtctt cagttgggcg gcaacgcaag ttgttacagc gatggaagca acccataaat
    33301 tgggcggtga aaactatgtc ctgtggggtg gtcgtgaagg ttacgaaacg ctgttaaata
    33361 ccgacttgcg ccaggagcgt gaacagctgg gccgctttat gcagatggtg gttgagcata
    33421 aacataaaat cggtttccag ggcacgttgc ttatcgaacc gaaaccgcaa gaaccgacca
    33481 aacatcaata tgattacgat gctgcgacgg tctatggctt cctgaaacag tttggtctgg
    33541 aaaaagagat taaactgaat attgaagcta accacgcgac gctggcaggt cactctttcc
    33601 atcatgaaat agccaccgcc attgcgcttg gcctgttcgg ttctgtcgac gccaaccgtg
    33661 gcgatgcaca actgggctgg gacaccgacc agttcccgaa cagtgtggaa gagaatgcgc
    33721 tggtgatgta tgaaattctc aaagcaggcg gtttcaccac tggcggtctg aacttcgatg
    33781 ccaaagtacg tcgtcaaagt actgataaat atgatctgtt ttacggtcat atcggcgcga
    33841 tggatacgat ggcgttggcg ctgaaaattg cagcacgcat gattgaagat ggcgagctgg
    33901 ataaacgcat cgcgcagcgt tattccggct ggaatagcga attaggccag caaatcctga
    33961 aaggccaaat gtcactggca gatttagcca aatatgctca ggaacacaat ttgtctccgg
    34021 tgcatcagag tggtcgccag gagcaactgg aaaatctggt aaatcattat ctgttcgaca
    34081 aataacggct aactgtgcag tccgttggcc cggttatcgg tagcgatacc gggcattttt
    34141 ttaaggaacg atcgatatgt atatcgggat agatcttggc acctcgggcg taaaagttat
    34201 tttgctcaac gagcagggtg aggtggttgc ttcgcaaacg gaaaagctga ccgtttcgcg
    34261 cccgcatcca ctctggtcgg aacaagaccc ggaacagtgg tggcaggcaa ctgaccgcgc
    34321 aatgaaagct ctgggcgatc agcattctct gcaggacgtt aaagcattgg gtattgccgg
    34381 ccagatgcac ggtgcaacct tactggatgc ccagcaacgg gtattacgcc ctgccatttt
    34441 gtggaacgac gggcgctgtg cgcaagagtg cactttgctg gaagcgcgag ttccgcaatc
    34501 acgggtgatt accggtaacc tgatgatgcc cggatttact gcgcctaaat tgctatgggt
    34561 tcagcggcat gagccggaga tattccgtca aatcgacaaa gtattattac cgaaagatta
    34621 cttgcgtctg cgtatgacgg gggagtttgc cagcgatatg tctgacgcag ctggcaccat
    34681 gtggctggat gtcgcaaagc gtgactggag tgacgacatg ctgcaggctt gccacttatc
    34741 tcgtgaccag atgcccgcat tatacgaagg cagcgaaatt actggtgctt tgctacctga
    34801 agttgcgaaa gcgtggagta tggcggcggt gccagttgtc gcaggcggtg gtgataatgc
    34861 agctggtgcg gttggtgtag gaatggttga tgctaatcag gcaatgttat cgctggggac
    34921 gtcgggggtc tattttgctg tcagcgaagg gtttttaagc aagccagaaa gcgccgtaca
    34981 cagcttttgc catgcgctac cgcagcgttg gcatttaatg tctgtaatgc tgagtgcagc
    35041 gtcgtgtctg gattgggccg cgaaattaac cggcctgagc aatgtcccag ctttaatcgc
    35101 tgcagctcaa caggctgatg aaagtgccga gccagtttgg tttctgcctt atctttccgg
    35161 cgagcgtacg ccacacaata atccccaggc gaagggggtt ttctttggtt tgactcatca
    35221 acatggcccc aatgaactgg cgcgagcagt gctggaaggc gtgggttatg cgctggcaga
    35281 tggcatggat gtcgtgcatg cctgcggcat taaaccgcaa agtgttacgt tgattggtgg
    35341 cggggcgcgt agtgagtact ggcgtcaaat gctggcggat atcagcggtc agcagctcga
    35401 ttaccgtacg gggggggatg tggggccagc actgggcgca gcaaggctgg cgcagattgc
    35461 ggcgaatcca gagaaatcgc tcattgagtt gttgccgcag ttaccgttag aacagtcgca
    35521 tctaccagat gcgcagcgtt atgccgctta tcagccacga cgagaaacgt tccgtcgcct
    35581 ctatcagcaa cttctgccat taatggcgta aacgttatcc tctgcctgac cgggtggggg
    35641 ataattcaca tctatatatc tcagtaatta attaatattt agtatgaatt tattctgaaa
    35701 atcatttgtt aatggcattt ttcagttttg tctttcgttg gttactcgta atgtatcgct
    35761 ggtagatatg gagatcgtta tgaaaacctc aaagactgtg gcaaaactat tatttgttgt
    35821 cggggcgctg gtttatctgg ttgggctatg gatctcatgc ccattgttaa gtggaaaagg
    35881 ctattttctt ggcatattaa tgacagcaac ttttggcaac tatgcatatc ttcgcgcaga
    35941 aaaactcggg caactggata atttttttac ccatatctgc cagttagttg cgttaatcac
    36001 tatcggtctc ttgtttatcg gtgttttaaa cgcacctatc aatgcttatg aaatggtgat
    36061 ctaccctatc gccttttttg tctgcttgtt tggtcaaatg cgtttgtttc gctcggcatg
    36121 agcaacataa agctcttaca cattcaggaa tgaaggaata ctgtgatgga caacaaaata
    36181 tcaacctatt ctccggcatt cagtattgtg tcatggatag ctctcgttgg tggtatcgtt
    36241 acctatctgt tagggctatg gaatgcagag atgcagttaa atgaaaaagg atattatttt
    36301 gccgtactgg tattaggact gttttctgcg gcgtcttatc aaaagaccgt tcgggacaag
    36361 tatgaaggca taccgaccac ctccatttat tatatgacct gcctgactgt ctttattatc
    36421 tctgttgcgt tactgatggt aggtctgtgg aatgcaacgt tattactcag cgagaaaggt
    36481 ttttatggac tggctttctt cttaagcttg tttggtgcag tagcggtgca gaagaatatt
    36541 cgtgatgccg gaataaaccc accaaaagaa acacaggtta cccaggaaga atacagcgaa
    36601 taattcacat aagcccggtc agtccaatgt gaccggcctt ttacttaact cactagtctg
    36661 tttctgtcga ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa cgtcgcgcaa
    36721 aagatccaaa taatatcgag tattggccag tttttaagct caatcccccg ggtgcgcagt
    36781 gcatggataa tcaaggcgtg gaacccgtat atgcccaacg aatggcggga gattaagcca
    36841 agtccgcgaa cggtacgcgt atcaagcgtg tttttaacca gagtcaatag cgtgattgcg
    36901 cagataaaaa ccatcggccc acagtaaaga taccaggtat cggcaaaatt tccgcgccac
    36961 tgcaattcat ataatgtccc gcgagagata ataaaaaccc ccgtcgcaaa cagtgctgcg
    37021 cacacccacg acagtgcttt atgctgtgtg tccatcatcc ctatagcgcg gcccaacatg
    37081 ccatacagga tgtagtaaaa agtatcgcca ttgatatata agttaattgg cagccattca
    37141 aaaccgtcaa ttttctgcgg cactgtgttt gggttagcga taataccaat caccaccatt
    37201 agcaccagca acatttttcc gccgacgttc ttcacctgaa tcagcggtga aaccagataa
    37261 atcaccgcaa tcgcgaagaa aaaccataag tggtaaaaca ctggcttttg cagcaggttt
    37321 ttcagcgcta actccacatt gatggaggta aacagcgcaa tgtagagcag tgcgattgcg
    37381 ctataaaaaa gcagacataa gccgatacgc aagaaatggc gcggctgggc gctgcgttcg
    37441 ccaaaaaaga gatagccgga aatcatgaaa aatagcggca cgctgacacg agaggcagaa
    37501 ttcagaacat tggcgatatc ccaggtgacg gggctaacac tatgagcatt ggtcacatac
    37561 caggtagtgg tgtgaatcat caccaccatt aaacacgcta tccctcgcag gttatcaatc
    37621 cagtaaattt tgggctgcat ctgtgtctct gtatctggtt aaaaaagtct gaccgataaa
    37681 tcattcggtt ggcgcactgg aataatctga gttttatcac tacagcttat agaggcttaa
    37741 ggaaattcgt aagatatcag ccactatacc gatataaata ataagactca cctgcaaacc
    37801 agacggtaat ttaatgatga tgaactcttt ctttccggca atggcgctta tggtgcttat
    37861 ggcgctagtg ggttgttcta caccgccacc cgtgcagaaa gcacaacggg taaaggttga
    37921 tcctctgcgt tcgttgaata tggaagcgtt atgcaaggat caggcggcga aacgttataa
    37981 caccggcgag caaaaaatcg acgtcaccgc atttgaacag ttccagggaa gctacgaaat
    38041 gcgcggttat accttccgta aagagcagtt tgtctgttct tttgacgcag atggccattt
    38101 tttgcatctt tccatgcgtt aagccctgct ttttcccgtt tcgtactgta tatcttccat
    38161 ccagcgggta tactgatccc ttcctttaaa tccacacgta tccagcacga aataatatgc
    38221 aaaagtttga taccaggacc ttccagggct tgatcctgac cttacaggat tactgggctc
    38281 gccagggctg caccattgtt caaccattgg acatggaagt cggcgcggga acctctcacc
    38341 caatgacctg tctgcgcgcg ctggggccag aaccgatggc ggctgcttat gttcagcctt
    38401 ctcgtcgccc gaccgatggt cgctacggcg aaaaccccaa ccgtttacag cactactatc
    38461 agttccaggt ggtcattaag ccatcgccgg acaatattca ggagctgtac cttggttctc
    38521 tgaaagagct gggcatggac ccgaccatcc acgacatccg tttcgtggaa gataactggg
    38581 aaaacccgac gctgggtgcc tgggggctgg gctgggaagt gtggctgaac ggcatggaag
    38641 tgacgcagtt cacttacttc cagcaggttg gtggtctgga gtgtaaaccg gttaccggcg
    38701 agatcaccta cggtctggaa cgtctggcaa tgtacattca gggcgtagac agcgtttacg
    38761 acctggtctg gagcgacggc ccgctgggta aaaccaccta tggcgacgtg ttccatcaga
    38821 acgaagtgga gcagtccact tacaacttcg aatacgcgga tgtggacttc ctgttcacct
    38881 gctttgagca gtacgagaaa gaagcgcagc agctgctggc gctggaaaat ccgctgccgc
    38941 tgccagccta cgagcgtatt ctgaaagccg cccacagctt caacctgctg gatgcgcgta
    39001 aagccatctc cgtcaccgag cgtcagcgct atattctgcg cattcgcacc ctgaccaaag
    39061 cagtggcaga agcatactac gcttcccgtg aagccctcgg cttcccgatg tgcaacaaag
    39121 ataagtaaga ggcggctatg tctgagaaaa cttttctggt ggaaatcggc actgaagagc
    39181 tgccaccaaa agcactgcgc agcctggctg agtcctttgc tgcgaacttt actgcggagc
    39241 tggataacgc tggcctcgca cacggcaccg ttcaatggtt tgctgctccg cgtcgtctgg
    39301 cgcttaaagt agctaacctg gcggaagcgc aaccggatcg tgaaatcgaa aaacgcggcc
    39361 cggcgattgc ccaggcgttc gacgctgaag gcaaaccgag caaagcggca gaaggctggg
    39421 cgcgtggttg cggtattacc gttgaccagg ctgagcgtct gactaccgat aaaggcgaat
    39481 ggctgctgta tcgcgcccac gtgaagggcg aaagcactga agcactgctg ccgaatatgg
    39541 ttgcgacttc gctggcgaaa ctgccgatcc cgaaactgat gcgttggggt gcaagcgacg
    39601 tgcacttcgt gcgtccggtg cacaccgtga ccctgctgct gggcgacaaa gttattccgg
    39661 ctaccattct gggcattcag tccgatcgcg tgattcgcgg ccaccgcttt atgggcgagc
    39721 cggaattcac tatcgacaat gctgatcagt atccggaaat tctgcgtgag cgcgggaaag
    39781 tcatcgccga ttacgaagaa cgtaaggcga agattaaagc cgatgccgaa gaagcggcgc
    39841 gtaagattgg cggtaacgct gacttaagcg aaagcctgtt ggaagaagtc gcttcgctgg
    39901 tggaatggcc ggttgtgctg accgcaaaat tcgaagagaa attcctcgcg gtgccgtctg
    39961 aagcgctggt ttacaccatg aaaggtgacc agaaatactt cccggtgtat gcgaacgacg
    40021 gcaaactgct gccgaacttt atcttcgttg ccaacatcga atcgaaagat ccgcagcaaa
    40081 ttatctctgg taacgagaaa gtcgttcgtc cacgtctggc ggatgccgag ttcttcttca
    40141 acaccgaccg taaaaaacgt ctggaagata acctgccgcg cctgcaaacc gtgctgttcc
    40201 agcaacagct gggtacgctg cgtgacaaga ctgaccgcat ccaggcgctg gctggctgga
    40261 ttgctgaaca gattggcgct gacgttaacc acgctacccg tgcgggcctg ctgtccaagt
    40321 gcgacctgat gaccaacatg gtcttcgagt tcaccgacac ccagggcgtt atggggatgc
    40381 actacgcgcg tcacgatggc gaagcggaag atgttgccgt ggcgctgaac gagcagtatc
    40441 agccgcgctt tgccggtgat gacctgccgt ctaacccggt agcctgtgcg ctggcgattg
    40501 ctgacaagat ggatactctg gcgggtatct tcggtatcgg ccagcatccg aaaggcgaca
    40561 aagacccgtt tgcgttgcgt cgtgccgcac ttggcgtgct gcgtattatc gttgagaaga
    40621 acctcaacct tgatctgcaa acgctgaccg aagaagcggt gcgtctgtat ggcgataagc
    40681 tgactaatac caacgtagtt gatgatgtta tcgactttat gctcggtcgc ttccgcgcct
    40741 ggtatcagga cgaaggttac accgttgata ccatccaggc ggtactggcg cgtcgtccga
    40801 ctcgtccggc agatttcgat gcccgtatga aggcggtatc gcacttccgt actctggatg
    40861 cggcagcgtc actggcggcg gcaaacaagc gtgtatctaa cattctggcg aagtctgacg
    40921 aagtgctgag cgatcgcgtg aatgcttcta ctctgaaaga gccggaagaa attaaactgg
    40981 cgatgcaagt tgtggtgctg cgcgacaagc tggagccgta cttcgctgaa ggtcgttacc
    41041 aggacgcgct ggtcgaactg gctgaattgc gtgaaccggt tgatgccttc ttcgataaag
    41101 tgatggtcat ggttgatgac aaagaattgc gaatcaaccg tcttaccatg ctggagaaac
    41161 tgcgcgaatt attcctgcgc gttgcggata tttcgctgtt acagtaataa cgccgttatt
    41221 aaaaagcctg ccatctggca ggctttttta tcgctaaata atagagcaac ctttaataat
    41281 cttctgctga ataaagattg tctcatatat taattttatg agattttttt aggattatat
    41341 caaggagaag aaacgaactt attaagctag aatagctgtg ggtgcttgag actgtttgtc
    41401 tcaggtattc accgaaaggc agacagagaa aagccccacc tgactataaa tcaaagtgag
    41461 gctaccctat gcctgaacac cataaggtta gcctcttact cgttggaaat caacacagga
    41521 ggctgggaat gccgcagaaa tatagattgc tttctttaat agtgatttgt ttcacgcttt
    41581 tattttttac ctggatggta agagattcac tgtgtgaatt gcatattaaa cagggaagtt
    41641 atgagctggc ggcattttta gcctgcaatt tgaaagagta agagactttg gcgggaagtt
    41701 attcccgcct ttcttacggc gttgcgcatt ctcattgcac ccaaatttat tcttcacaaa
    41761 aataacacta gattctatta cgcgatcgat tatttatttc ctgaaaaaca aataaaaaaa
    41821 tccccgccaa atggcaggga tcttagattc tgtgctttta agcagagatt acaggctggt
    41881 tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca ctttctgacc
    41941 ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga acacgtcttt
    42001 agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc attttacgat
    42061 accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc ctttcggcga
    42121 tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag gggtatcaac
    42181 gataactctt gaagggactt gccttactac actgctttaa tggtctgtac gtcaaaccgt
    42241 tgatgtgcat taagccacgc attggcgggt gatgcaacaa ttatttttca tatttatgat
    42301 taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag tggataaaag
    42361 tctattccat caactgctta cttaatgccg ggttggcttg aatcaaacgc atcaatttta
    42421 gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg gcgactgata
    42481 cgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt cccttttcgt aactgctcaa
    42541 tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca tctttaaaaa
    42601 caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc attgaaaact
    42661 cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat agtcgggaaa
    42721 aacagacata gcggggtgta gtcagggcat gctctggcta tgattcgatt cagccagtca
    42781 tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct cttaccactc
    42841 ccggtttgta aagatttcga aaatctaacg ttctgcgaag gcgtaaatct caggctacaa
    42901 aaaaagcaac ggcactatca ttttttaaca atgaaacaac aaattgacct tgtttcattt
    42961 tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc tgtcctggac
    43021 tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt gaaaggagta
    43081 agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg gcgttattaa
    43141 tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa gcgtttatcg
    43201 acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg actgccgatc
    43261 agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt tattctcagc
    43321 aggtgaatca ggcgatggat tccggtctgc gcccggttgt agacagcgtt aacgcaattc
    43381 gcgtaccgca ggattacgtt acgcaaagcg gcccactgcg tgaaatgaac ggttctctgg
    43441 gcgtactggc gcaacagctg caaaatgcga agttgcaggc cgatgctgcg cactctgcgc
    43501 taaaacagag cgatgacctg aaaccggtct tcgatcaggc attcaccaaa gtggtaacga
    43561 cgccagctga tgcgttgcaa ccgttgattc cggcggcaca aactttcacg caacaactgg
    43621 tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc gcaaatggca
    43681 tccagttccc gacttcgcag caggcaagtg aatataacaa actgattgcg ccattaccgg
    43741 cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc caataaagag
    43801 taaaagcccg agcgattatc gggctttact ccacgcagtc gcggcttagt ccgcgacgtg
    43861 cggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaagt tatccacggc
    43921 acaggcggcc atgccataac gcgtctcatg agtggcagat ccaatatgcg gtactgcgac
    43981 gacgttggcc attgagagca acggcgaatc tactgacagt ggctcttgtt cgaagacatc
    44041 cagcccggca gcgtgaattt cccctttctg caatgcagca atcagtgcat tttcatcaac
    44101 caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg cgaattgttc
    44161 tgcgccaaac agatgatgcg tttcatcagt taacggcagg atcaggcaaa cgaaatctga
    44221 ctcttgtaac agtgtatcca aatcgcagta gcgggcgttg aagcgttctt ctgcttcttt
    44281 atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgcg cacgttgtgc
    44341 cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt gaacgtcagt
    44401 gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttactc gttctgctac
    44461 ctccacaacc cgacgagcgg tagacaatac cagcgccatc agcgtatcgg cgacggtttc
    44521 tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg catcgacatc
    44581 aaaattgtca tagcctacgg agatcgttga tgtggcacgc agtttcggca ttttttccag
    44641 caatgcggca tcaacattct cgtttgaacc cagtaaacct tcagcttcgg caagaattgc
    44701 tgcattttgt tcgatggttt gtgggctgag gtttgccacc tggtgaacgg tgaaatgctc
    44761 ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg acggcttcat
    44821 tcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg aaggaaaact
    44881 gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc gggcttaagg
    44941 taatttctac acggcggttt tgcgccttac cttctgcagt gctgttgctg gcgattgggt
    45001 tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg gtgatcaacg
    45061 cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg tgcccacccg
    45121 tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc agtaccattg
    45181 ccacgccggt cagggtgtta gcgcccgccg gtttcagggt tgcgctgctg ctgtcgaagg
    45241 tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg ctaacaccag
    45301 tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa cccacgccgc
    45361 cgcccagagc tgcgcctgct gctgcgccaa tgagcgcgcc tttaccgcga tctttcttcg
    45421 aagaagagag cgcaccaata cccgcgccaa cgagagagcc cagacctgcg ccgatagcag
    45481 atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt acagccagat accgccagag
    45541 cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat ccttaataac
    45601 ctttttattc tttgccacgg gttctgtggc gggagattat gccgcgtgaa catgaagatt
    45661 attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac atctaacctt
    45721 tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac tgccgcccac
    45781 tgggggccta tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg tggcgcgtta
    45841 gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca cagcaatacg
    45901 cgggtgcgat ttccaatggt gcgcaaaggc tttcttgcgt caccggaaaa tccgcaaggc
    45961 attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga gcttattcac
    46021 catcaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc tggttcctac
    46081 ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg ctatatggcg
    46141 ctggcaggcg gttatactgg gcatctgggg gattattcga ccggcgcggc acaggcgatc
    46201 atgccgtatg tcgtgggtgg cagcgaagtt tatcaacagc aaaccagttg gccgctggtg
    46261 ctggaacata gcgatgtcat ggtgctgtgg agtgctaacc cactcaatac gctgaaaatt
    46321 gcgtggaatg catccgatga gcaggggctt tcttactttt ccgcactgcg tgacagcggg
    46381 aaaaagctga tctgcattga tccaatgcga tcggaaacca tcgatttctt tggcgataaa
    46441 atggagtggg tggcaccaca catgggcacc gatgttgcgt tgatgctggg gatcgcccat
    46501 acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcgttgcac cacaggttat
    46561 gccgtcttcg cctcttattt gctgggcgag agtgatggta tagcgaaaaa cgccgaatgg
    46621 gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg agctggcggc tattttccac
    46681 caaaatacaa ccatgctgat ggcaggctgg ggaatgcagc gccaacagtt tggtgagcaa
    46741 aaacactgga tgctcgtcac gctggcggca atgttggggc aaatcggcac acccggcggc
    46801 ggttttggtc tttcttacca ttttgccaat ggtggtaacc ccacgcgccg cgctgcggtg
    46861 ctctcttcca tgcagggcag tttgcctggc ggcaccgatg cggtggataa aatccctgtt
    46921 gcccgcattg ttgaagcact ggaaaacccc ggtggcgcat atcaacataa cggtatggac
    46981 cgacatttcc cggatattcg ttttatctgg tgggcgggtg gtgccaactt tactcatcat
    47041 caggatacca atcgcctgat ccgtgcctgg caaaaaccgg agctggtggt gatctctgaa
    47101 tgcttctgga cggcggcggc gaaacacgcg gatatcgttc tgcctgcgac cacctctttt
    47161 gagcgtaatg atctcaccat gaccggcgat tacagtaatc agcatctggt gccgatgaag
    47221 caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc cgagttaagt
    47281 gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtga gctgcaatgg
    47341 ctggaaacgt tttataacgt tgcccggcaa cgcggggcaa gccagcaggt tgaattgccg
    47401 ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa cccggacagc
    47461 gagcggttta ttcgcttcgc cgattttcgc cgcgatccgc aggcgcatcc attaaaaacc
    47521 gccagcggca agattgaaat cttctcgcag cgtattgccg attacgctta tccggattgc
    47581 cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga accggaacag
    47641 ttgcaggtac tttctgctca tccggcacat cgtctgcaca gccagctgaa ttacagttct
    47701 ctgcgcgaat tgtacgcggt ggcaaaccgt gagcctgtca ccattcatcc tgacgatgct
    47761 caggcgcgcg gcatacaaga tggcgatact gttcggctgt ggaacgcacg cgggcaaatt
    47821 cttgccgggg cagtcattag cgagggaatt aaacctggcg tgatttgcat tcacgaaggg
    47881 gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc agtgaacgtg
    47941 ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa tacggcgctg
    48001 gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga accaccggcc
    48061 agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcagtcg acaatgtgaa
    48121 aaccctgcgc ctggtaaaaa tctatcgccg gttgattttt ttgataaacc tccagcatca
    48181 gatggggatg gcgctgctgc acatactgca ttagcgcctt accaataccg cgtctgacgg
    48241 ccttcggtgc gacaaacatt gcagccagaa atcggccttc cataatgctg acaaaaccga
    48301 gaagcttacc gtcttcttcc cagacccagt tttgcgcgtt ggcaagatac gcatcgcgca
    48361 ccagcggaat gcagtcacgc cagtaactcg attttataaa gggatgcccc caggttgtac
    48421 tttccagcca cagttcgagg atcgcgggga gttctgaatg ttgcacttcc cgaatcatgg
    48481 tttatttccc ggatggcaac agcagccaac cacatgatca ttcaccagcc cacatgcctg
    48541 cataaaggag taacagattg tggtaccgac aaacttaaaa ccacgttttt ttagtgcctt
    48601 agatagggcg tcggaggcgg gtgtagatgt ggggatttcg ctcaacgttg tggcttgtgt
    48661 cacctgtggt tgatgattga caaacgacca gacaaagtcg gcaaacggtt cgccgttctg
    48721 ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc cgcgatggcg
    48781 gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg cagccacctt
    48841 caccggatcg aactgatgaa agctggcgcg atagttttcg cgttttttga ggacggtgat
    48901 ccacgataat ccagcctgct gcccttcaag gcagatcatt tcgaacagtt ttttactgtc
    48961 agtttcaggc acgccccact cattatcatg gtaggcaata taaagcgggt cctgactcac
    49021 ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa tttcagccgc
    49081 ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat gcccgatatc
    49141 cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg tgttaatgat
    49201 cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagtgtgg taatcagtgc
    49261 cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata cgcaacccgg
    49321 acattccaca gagcgttaca cctgggatag cgatcatcaa cctgattaca acgatatttt
    49381 gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc tggcggaaga
    49441 aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt tatatgagca
    49501 ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct cgatgtataa
    49561 cgagtttggc gacagtacta ccacgctggc cgatccgtta tggcatgcca gcgtgagtac
    49621 cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc aaatcagcta
    49681 taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc ggatgacggc
    49741 gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt tgctcaatca
    49801 aaatattgct gcctatgctg cgctatctca ggcagaaaat accactaaca atagcgacta
    49861 tctgtatacg atgggggtta gcgccagatt ttaacgttat agatacaatt gaaaccaata
    49921 aataacaact aatgtggcga tagtgggtga taacttacta aataataaat ttggtgaata
    49981 attgtcgagt cattcattcc tgaactaatg catttcattc cgttctgatg gcatttcatg
    50041 ccgtttttcc ccaggcataa agtgcacttc gttatggttg tcagcagaga tttttccttt
    50101 ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc gctggttgac
    50161 actcatcggt actatcatta cccagtttgc ccttgggtcg gtttatacct ggagcctgtt
    50221 taacggcgcg ctttctgcca agctggatgc gccggtaagc caggtcgctt tctctttcgg
    50281 cttgttaagt ctggggctgg caatttcatc ttctgttgcg gggaaattac aggaacgttt
    50341 tggcgttaaa cgcgtcacta tggcttccgg cattttgctg ggattaggct tcttcctgac
    50401 agcgcattct gacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg tcggtctggc
    50461 ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc cggagcgtaa
    50521 aggtctgatc tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg gtttcaaatt
    50581 tatcgacacg cagctgctgg aaacggtggg tctggaaaaa acctttgtga tttggggagc
    50641 gattgcgctg gtgatgattg tctttggcgc aacattaatg aaagacgcac caaaacagga
    50701 agtgaaaacc agcaatggtg tggtggagaa ggactacacc ctggcagagt cgatgcgtaa
    50761 accgcagtac tggatgttag cggttatgtt cctgactgcg tgcatgagtg gtctgtatgt
    50821 gattggtgta gcgaaagata tcgctcaaag tctggcgcat cttgatgcaa tttccgcagc
    50881 caatgctgtg acggttattt ccatcgccaa cctttctggt cgtctggtgc tgggcattct
    50941 gtctgacaaa atcgcccgta tccgtgttat caccattggt caggtgatat cgctggtggg
    51001 tatggcggcc ctgctgtttg caccattgaa tgcagtgacg ttctttgcag cgattgcctg
    51061 cgtggcattt aactttggcg gcactatcac ggtgttcccg tcactggtca gtgagttctt
    51121 cggcctcaat aacctggcga aaaactacgg tgtgatttat ctcggtttcg gtatcggcag
    51181 catttgtggg tcgattatcg cctcactgtt tggcggcttc tatgtgacct tctacgtgat
    51241 tttcgcactg ctgattctgt cactggcgct ctctacgacg attcgtcagc cagagcagaa
    51301 aatgttgcgt gaggcgcatg gctcccttta atcatctgcc gcgagctact gggctttagc
    51361 cttagtgtga atcaaacgct tcaacgcaat gtataaacaa tgacgcgtaa aatttgggcc
    51421 agttaactgg ccctttttac tgctttttgt aacgctaaag tctcttttca aacttgcatt
    51481 tttgtaaatt tgtgcttcat gcacactctt tccccacact ttttcccttt gctgtggtct
    51541 acttattcgc gcgtgtagat tttacttatc tgactacctc cgcacttttc ccctgccggg
    51601 cctgaaaagc cgctaagcag ggtgttatca cctgtttgtc cagggtttgt ttgcatgaga
    51661 tacatcaaat cgattacaca gcagaagctg agctttttgc ttgcaatcta tattggcctt
    51721 tttatgaatg gcgcggtttt ttaccgccgc ttcggcagct atgcgcacga ttttaccgtc
    51781 tggaaaggca tttctgctgt tgttgaactg gccgccaccg tactggtgac cttcttttta
    51841 ctacgtcttc tttcgctgtt tggccgccgt agctggcgta ttctggcgtc gctggtggtg
    51901 ctcttttccg caggtgccag ctattacatg accttcctta atgtggtcat tggttatggc
    51961 atcatcgctt ccgtcatgac caccgatatc gacctgtcaa aagaagttgt tggtctgaac
    52021 tttattctct ggttaatcgc cgttagcgcg ttgcctctta tccttatctg gaataaccgc
    52081 tgtcgttaca ccttgctccg acaactgcga accccagggc agcgtattcg cagcctggcg
    52141 gtcgtcgtac tggcaggtat tatggtttgg gcaccgattc gtttgctgga tatccagcag
    52201 aagaaagtgg agagggcgac aggtgttgat ttgccgagtt atggcggcgt cgtcgcgaac
    52261 tcttatctgc catcaaactg gctttcggca ttaggtcttt atgcctgggc gcgggtcgat
    52321 gaatcttccg ataacaattc tctccttaac cctgcgaaga agtttacgta ccaggctcca
    52381 caaaacgttg atgatacata tgtcgttttc attattggtg aaaccacgcg ctgggatcat
    52441 atgggcattt tcggctatga acgcaatacc acaccgaagc tggcccagga aaaaaatctg
    52501 gcggcgttcc gtggttactc ctgtgatacc gcaaccaaac tctctctgcg ttgcatgttt
    52561 gtacgtcagg ggggcgcgga agataatccg cagcgcacat taaaagaaca gaacattttc
    52621 gcggtattga agcagttagg attcagttct gatctctacg ctatgcaaag cgaaatgtgg
    52681 ttctacagca atacgatggc ggacaacatt gcttatcgtg agcagattgg tgcggagcca
    52741 cgtaaccgtg gcaaaccggt agatgatatg ttgctggtag acgaaatgca gcaatcgctg
    52801 gggcgcaacc cggatggtaa gcatctgatc attctgcata ccaaaggctc acactttaac
    52861 tacacccagc gttacccgcg cagctttgcg cagtggaagc cggaatgtat tggtgttgat
    52921 agcggctgta ccaaagcgca gatgatcaac tcatacgaca actcggtgac ctatgtggat
    52981 cactttattt ccagcgtgat tgatcaggtt cgcgataaga aagcgattgt gttctatgca
    53041 gctgaccatg gtgagtcaat taatgaacgc gagcacctgc acggcacgcc gcgtgaactg
    53101 gcaccgccgg aacagttccg cgtgccgatg atggtctgga tgtcggataa atatctggaa
    53161 aatccggtca atgcgcaggc atttgcgcag ctgaaaaaag aagctgacat gaaagtgcca
    53221 cgccgtcacg tagagctgta cgacaccatc atgggttgtc ttggctatac ttcaccggat
    53281 ggtggaatta acgaaaacaa caactggtgc cacatcccgc aggcaagaga ggcggcggct
    53341 aactaagagg cctgctgact ttctcgccga tcaaaaggca ttttgctatt aagggattga
    53401 cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg tgattggcgc agcctggtag
    53461 cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc tctatcaccg accaaattcg
    53521 aaaagcctgc tcaacgagca ggcttttttg catttgtagc ctatgaggat gagaacctcc
    53581 gggggcaggg aggttcgact cgagcgaagc gagagaacgt tgcgccagca acggcccgca
    53641 gggcgagcca cgaagtggcg agtaatcctc tatcaccgac caaattcgaa aagcctgctc
    53701 aacgagcagg cttttttgca tctgtagctc atgaggaaga gaacccccgg gggcggggag
    53761 gttcgactcg acgtaggtct gataagacgc gccagcgtcg catcaggcaa cggcaccgca
    53821 cttattgtcg gatgcggcgt gaacgcctta ttcgatctac acaccacaca tttccctgtc
    53881 aaatttgcgc cccatatctc actatccatt aacggatttg tgacagaaac cattgtcttt
    53941 ttttatattt gcttaaatct ttttttgcgt tacttatggc tggcgctggc attttccgct
    54001 gtgcgtttta gcgttcatgg tattaattgc tcagatatcc accttttcct cagatttttt
    54061 tcattaaatg cataaaactt aatcaccaaa tgttgctaaa tattaagctg attgttctgg
    54121 ttgtgttggc gtagcacaaa tttctctgct gcaaatagct gtttgaagtt tttttaatct
    54181 ttgtttgtgg attctcaccg ttggtgtaaa tcccggttgg ctgtattgac gttttcacat
    54241 tctgttgaca gattgtaggt cacgaggggc attttatgga ggatccgcac tgttacactg
    54301 atgttaatta gtacggcatc cccacctcat aacgttgacc cgaccgggca aaaaacaaaa
    54361 aaggtcaggc agcgacaacc cactgcaaag ggttaaaaca acaaacatca caattggagc
    54421 agaataatgc gtatttcctt gaaaaagtca gggatgctga agcttggtct cagcctggtg
    54481 gctatgaccg tcgcagcaag tgttcaggct aaaactctgg tttattgctc agaaggatct
    54541 ccggaagggt ttaacccgca gctgtttacc tccggcacca cctatgacgc ctcttccgta
    54601 ccgctttata accgtctggt tgaatttaaa atcggcacca ctgaagtgat cccgggcctc
    54661 gctgaaaagt gggaagtcag cgaagacggt aaaacctata ccttccatct gcgtaaaggt
    54721 gtgaagtggc acgacaataa agaattcaaa ccgacgcgcg aactgaatgc cgatgacgta
    54781 gtgttctcgt tcgatcgtca gaaaaacgcg caaaacccgt accataaagt ctctggcggc
    54841 agctacgaat acttcgaagg catgggctta ccagagctga tcagcgaagt gaaaaaggtg
    54901 gacgataaca ccgttcagtt tgtgctgact cgcccggaag cgccgttcct cgctgacctg
    54961 gcaatggact tcgcctctat tctgtcaaaa gaatatgctg acgcgatgat gaaagccggt
    55021 acaccagaaa aactggacct caacccaatc ggtaccggcc cgttccagtt acagcagtac
    55081 cagaaagatt cccgtattcg ctataaagcg tttgatggct actggggcac caaaccgaag
    55141 atcgatacgc tggtcttctc tattactcct gacgcttccg tgcgttatgc gaaattgcag
    55201 aagaacgaat gccaggtgat gccgtacccg aacccggcag atatcgcccg catgaagcag
    55261 gataaatcca tcaacctgat ggaaatgccg gggctgaacg tcggttatct ctcgtataac
    55321 gtgcagaaaa aaccactcga tgacgtgaaa gttcgccagg ctctgaccta cgcggtgaac
    55381 aaagacgcga tcatcaaagc ggtttatcag ggcgcgggcg tatcagcgaa aaacctgatc
    55441 ccgccaacca tgtggggcta taacgacgac gttcaggact acacctacga tcctgaaaaa
    55501 gcgaaagcct tgctgaaaga agcgggtctg gaaaaaggtt tctccatcga cctgtgggcg
    55561 atgccggtac aacgtccgta taacccgaac gctcgccgca tggcggagat gattcaggca
    55621 gactgggcga aagtcggcgt gcaggccaaa attgtcacct acgaatgggg tgagtacctc
    55681 aagcgtgcga aagatggcga gcaccagacg gtaatgatgg gctggactgg cgataatggg
    55741 gatccggata acttcttcgc caccctgttc agctgcgccg cctctgaaca aggctccaac
    55801 tactcaaaat ggtgctacaa accgtttgaa gatctgattc aaccggcgcg tgctaccgac
    55861 gaccataaca aacgcgttga actgtacaag caagcgcagg tggtgatgca cgatcaggct
    55921 ccggcactga tcatcgctca ctccaccgtg tttgaaccgg tacgcaaaga agttaaaggc
    55981 tatgtggttg atccattagg caaacatcac ttcgaaaacg tctctatcga ataattaaaa
    56041 gccatacaag actgatggca aaggcaaaac tgcctgatgc gctccgctta tcaggcctac
    56101 gaaaactctg caatgtattg agtttgcacg attttgtagg tcggataagg cgtttgcgcc
    56161 gcatccggca taaacaaagc gctctttgtc aacaatctgc atacccggtg gcgctgtgcc
    56221 tctgcgtgca gcgccatccg gcagcaatac ttcattccct gttccacaag ggcgggaatt
    56281 gatttgtgag caatacagac acgcagttcc aggctgcggg tcattataga gaatccgggt
    56341 tatgttgcag tttattctcc gacgtttggg actcgtcatc cccacgttta tcggtattac
    56401 ccttctcaca tttgcctttg tccacatgat cccgggcgat ccggtgatga tcatggcggg
    56461 cgagcgtggg atctccccag agcgtcacgc gcagctgctg gctgaactcg gcttagataa
    56521 accgatgtgg cagcagtatc tccattacat ttggggcgta atgcacggcg atttaggcat
    56581 ttcgatgaaa agccgcatcc cggtatggga agagttcgtg ccgcgcttcc aggccacgct
    56641 ggaacttggc gtctgcgcga tgatttttgc tacggcagtc ggtattccgg ttggtgtgct
    56701 ggctgcggtt aaacgcggtt ccattttcga tcacacggcg gttggcctgg cgctgacagg
    56761 ttattcaatg cctatcttct ggtggggcat gatgctgatc atgctggttt cggtgcactg
    56821 gaacctgacg cccgtctccg gtcgcgtgag cgacatggtg ttcctcgatg attccaatcc
    56881 gttaaccggt tttatgctga tcgacaccgc catctggggt gaagacggta actttatcga
    56941 tgccgtcgcc catatgatct tgcccgccat tgtgctgggt actatcccgc tggcggtcat
    57001 tgtgcgtatg acacgctcct cgatgctgga agtgctgggt gaagattaca tccgcaccgc
    57061 gcgcgccaaa gggttgaccc gccttagggt gattatcgtc catgcgctgc gtaacgcgat
    57121 gctgccggtg gtgaccgtta tcggcctgca ggtgggaacg ttgctggcgg gggcgattct
    57181 gaccgaaacc atcttctcgt ggcccggtct ggggcgctgg ttgattgacg cactgcaacg
    57241 ccgcgactat ccggtagtgc agggcggcgt attgctggtg gcgacgatga ttatcctcgt
    57301 caacttgctg gtcgatctgc tgtacggcgt ggtgaacccg cgtattcgtc ataagaagta
    57361 aggggacatc atgtcacagg ttactgaaaa taaagtgatt agcgcaccgg tgccgatgac
    57421 cccgttacag gagttctggc actattttaa acgcaacaaa ggcgcggtcg tcgggctggt
    57481 ttacgtcgtc atcgtgctgt ttatcgcgat ctttgccaac tggattgcac cctataaccc
    57541 ggcggaacag ttccgcgatg cactgctcgc cccgccagcc tggcaggaag gcggcagcat
    57601 ggcgcacttg ctgggcaccg atgacgtagg ccgtgatgtg ctgtcgcgtc tgatgtacgg
    57661 tgcgcgattg tcactgctgg ttggctgtct ggtagttgtg ttatcgctga ttatgggcgt
    57721 tattctcggc ctgatcgccg gttacttcgg cggcctggtc gataacatca tcatgcgcgt
    57781 ggtcgatatc atgctggcgc tgccaagtct gctgttggca ctggtgctgg tggcgatttt
    57841 cggcccgtcg attggtaacg ccgcgctggc gctgaccttt gttgccctgc cgcactatgt
    57901 gcgcttaacc cgcgccgccg tgctggtgga agttaaccgc gattacgtta ccgcgtctcg
    57961 cgtggcaggt gccggggcga tgcgtcagat gtttattaac atcttcccga actgccttgc
    58021 gcctctgatt gttcaggcgt cgctcggttt ctctaacgcc attctcgata tggctgctct
    58081 tggtttcctc ggcatggggg cgcagccgcc aacgccggag tggggcacca tgctctccga
    58141 cgtgttgcag ttcgcgcaaa gcgcctggtg ggtcgtgacc ttcccgggtc tggcgatcct
    58201 gctgacggtg ctggcattta acctgatggg tgacggtctg cgtgacgcgc tcgatcccaa
    58261 actgaagcag taagaggttc gagatggcgt tattaaatgt agataaatta tcggtgcatt
    58321 tcggcgacga aagcgcaccg ttccgcgccg tagaccgcat cagctacagc gtaaaacagg
    58381 gcgaagtggt cgggattgtg ggtgagtccg gctctggtaa atcggtcagt tcgctggcga
    58441 ttatggggct gattgattat ccgggccgcg taatggcgga aaagctggag tttaacggtc
    58501 aggatttgca gcgtatctcg gaaaaagagc gccgcaacct tgtgggtgcc gaagtggcga
    58561 tgatcttcca ggacccgatg accagcctta acccgtgcta caccgtgggt ttccagatta
    58621 tggaagcgat taaggtgcat cagggcggta ataagagcac tcgtcgtcag cgggcgattg
    58681 acttgttgaa tcaggtcggt attcccgatc cggcttcgcg tctggatgtt tacccgcatc
    58741 agctttccgg cggcatgagc cagcgcgtga tgattgccat ggcgattgcc tgtcggccaa
    58801 aactgctgat tgccgatgaa ccaaccactg cgctggacgt gaccattcag gcgcaaatca
    58861 ttgaactact gctggagcta cagcagaaag agaacatggc gctggtgtta attacccatg
    58921 acctggcgct ggtggcggaa gcggcacata aaatcatcgt gatgtatgcc ggtcaggtgg
    58981 tggaaacggg cgatgcgcac gccatcttcc atgcgccgcg tcacccgtat actcaggcat
    59041 tgctgcgtgc gctgccggaa tttgctcagg ataaagaacg tctggcgtcg ttgccaggtg
    59101 ttgttcccgg caagtatgac cgcccgaacg gctgcctgct taacccgcgc tgcccctatg
    59161 ccactgacag atgtcgcgct gaagaaccgg cgctgaatat gcttgctgac gggcgtcagt
    59221 ccaaatgcca ttacccactt gatgatgccg ggaggccgac actatgagta cgcaagaggc
    59281 cacctcgcaa caaccgctgt tgcaggctat cgacctgaaa aaacattatc cggtgaagaa
    59341 aggtatgttc gcgccggaac gtttggttaa agcgctggac ggcgtttcgt ttaaccttga
    59401 acgcggcaaa acgctggcgg tagtagggga atctggctgc ggtaaatcga ccctcggtcg
    59461 gttgctgacg atgatcgaaa cgcccaccgg tggtgaactg tattaccaag ggcaggatct
    59521 gctcaagcac gatccgcagg cgcagaagct gcgtcggcag aaaatccaga ttgtcttcca
    59581 gaacccttac ggttcgctga atccacgtaa aaaagtcggg caaattcttg aggagccgct
    59641 gctgattaat accagcttaa gcaaagaaca gcgtcgggaa aaagccctgt cgatgatggc
    59701 gaaagtcggc ctgaaaaccg aacattatga ccgctatccg catatgttct ccggcggtca
    59761 gcgtcagcgt atcgccattg cccgaggtct gatgctcgac ccggatgtgg tgattgccga
    59821 tgagccggtt tccgcgctgg acgtctcagt gcgcgcgcag gtgctgaatc tgatgatgga
    59881 tttgcagcag gagttggggc tgtcttatgt ctttatctcg cacgacctgt cggtggtaga
    59941 gcacattgct gatgaagtga tggtgatgta cctgggccgc tgcgtggaga agggaacgaa
    60001 ggaccaaatc ttcaataacc cgcgtcatcc gtacactcag gcgctactct ccgcgacgcc
    60061 gcgcctgaac ccggacgatc gccgcgagcg catcaagctc accggtgaac tgccaagccc
    60121 actgaatcca ccgccgggct gcgcgtttaa cgcccgctgt cgtcggcgct tcggcccctg
    60181 cacccagttg cagccgcagc taaaagacta cggcggtcaa ctggtagctt gttttgctgt
    60241 tgatcaggat gaaaatccgc agcgttaact cacaaaaccg gggccgtgcg ctccggttta
    60301 ctcaatcagc ttcaacagtg gcgacaagca tagcaaaatg ccgtaaagca gaatcagcca
    60361 ggctttaaac ccgcgaagtt tttccagttg tgcgacttta tagatcagga aaaatggaat
    60421 gaggcacgac acaatgccat ataacgggct tcccaactga aagaacacca gcaccgatac
    60481 acgaaacgag acccaaatcg tcaacgtaat gacgataaaa gcgcagatcg ccagagtcag
    60541 catgcgtgag ttaattttct tggtatcaat aattcggctt aacagattga gaataatgcc
    60601 tttaatggcc tcgtggaaac cgagataaat gccaaagaat gcggtcagta cggcaaagat
    60661 attcagcacc gtcgaggtga tatgaatgat atgcccaggg atcacctgcg cggccagtgc
    60721 cagtgccgag atattttgtt caaaggcaga aacggcttct tcgtggctaa ttgagaaggt
    60781 aaacgaaaag gcaaaaaata ggatcaccgc gatgagcgtg acataactaa ttcggtgggt
    60841 acgcagcgcg agccgggttg ccagtacttt atccgcttcc cgtttgcggt aggcaatatt
    60901 catcgggttt agcacctgaa taaataccgc agaaaagaag caaaaaggaa tagtaagcag
    60961 gacgtcgcgg aagaagacgg aggcttgcgg gaaggcggtt atattggcga aattccagtg
    61021 cgggatcatc gcaaaaccga agacgacaat aatccctact ttgaccacca ccattggccc
    61081 ggaaatctta aacagtaatc gttcaccacc agacgcaatt gccaccagta cggcgaaaat
    61141 ggcgacttta taaagtagag attgtgaaag atcggcatcg gttaaaccga aggttttcag
    61201 gtacgaggcg ctgtcgaaaa ccacggagag agagtagata aaaatcccgt ggataatcat
    61261 caaaaagtag ataaccccga ggaaaattcc ccagttcttc cccaggtaat gactgataat
    61321 atcggtgtag tcattacagg aatcgctttc agaaagggtt tttaaataaa tgtcctgcac
    61381 tacccaggtg gcaggataag caatgatcgc tgcggtaata aatacccaaa ttcccttcaa
    61441 gccaatttgt actggcatca gcacggttcc ggcaccaatc gccatgccta tgcataataa
    61501 aacccagccg aagtcgtagc gtgtaaacgg cattttctga tcgtgtttcg gtagtgtgtt
    61561 gtgctgcata aagtcctgtc cagaaagaag tcggcctcgc aaaaatgaat aaacccagtt
    61621 tctcttgcgc aacaggtgga gaagtcgaat acgcagtatg taaaaaatga ggcaacagat
    61681 tcactgggga ggtcgcgagg aggcgcaatt ctacaaaatt gcgcatttca ctaccagtct
    61741 ggagtcagcc cggatatgaa ctgcaactaa tgcaaataaa gaaattaagc tggaaggcat
    61801 cacgcaatga ggaagattgc cgggtgctga cggtaaaaag caatcgtaaa atcagcggca
    61861 agccggaaag gttccggtga ggcgcaatgt tgcgggggct taatccctgg tggcattggt
    61921 tgctggagag agaaaacccc cgcacgttgc aggtatgcac ctgacaacat cacgggggct
    61981 aatcttgaac ctagaccact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg
    62041 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat
    62101 agctggcatt cttgccagtc tgatcgtgaa ctggctgaac aagcggaagt aacgtgtcag
    62161 acgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag ggataaaact
    62221 ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg
    62281 cacagcacag agtgaagaag tgacggattt cggagctgaa aaacagtcgt agaatcagcg
    62341 gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg gcttaatccc tggtggcatt
    62401 ggttgctgga gagagaaaac ccccgcatat tgcaggtatg cacctgacaa catcacgggg
    62461 gccattcttg aaccta
//
LOCUS       DAZGTJ010000019        61567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000019 DAZGTJ010000000
VERSION     DAZGTJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.019"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            53..616
                     /gene="srlA"
                     /locus_tag="ACHK8E_001526"
                     /gene_synonym="gutA"
     CDS             53..616
                     /gene="srlA"
                     /locus_tag="ACHK8E_001526"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP0343813.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            613..1572
                     /gene="srlE"
                     /locus_tag="ACHK8E_001527"
                     /gene_synonym="gutE"
     CDS             613..1572
                     /gene="srlE"
                     /locus_tag="ACHK8E_001527"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP0343814.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSSRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1583..1954
                     /gene="srlB"
                     /locus_tag="ACHK8E_001528"
                     /gene_synonym="gutB"
     CDS             1583..1954
                     /gene="srlB"
                     /locus_tag="ACHK8E_001528"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0343815.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1958..2737
                     /gene="srlD"
                     /locus_tag="ACHK8E_001529"
     CDS             1958..2737
                     /gene="srlD"
                     /locus_tag="ACHK8E_001529"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP0343816.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2843..3202
                     /gene="gutM"
                     /locus_tag="ACHK8E_001530"
     CDS             2843..3202
                     /gene="gutM"
                     /locus_tag="ACHK8E_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP0343817.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3269..4042
                     /gene="srlR"
                     /locus_tag="ACHK8E_001531"
                     /gene_synonym="gutR"
     CDS             3269..4042
                     /gene="srlR"
                     /locus_tag="ACHK8E_001531"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP0343818.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            4035..5000
                     /gene="gutQ"
                     /locus_tag="ACHK8E_001532"
     CDS             4035..5000
                     /gene="gutQ"
                     /locus_tag="ACHK8E_001532"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP0343819.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4997..6511)
                     /gene="norR"
                     /locus_tag="ACHK8E_001533"
     CDS             complement(4997..6511)
                     /gene="norR"
                     /locus_tag="ACHK8E_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP0343820.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6698..8137
                     /gene="norV"
                     /locus_tag="ACHK8E_001534"
     CDS             6698..8137
                     /gene="norV"
                     /locus_tag="ACHK8E_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP0343821.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8134..9267
                     /gene="norW"
                     /locus_tag="ACHK8E_001535"
     CDS             8134..9267
                     /gene="norW"
                     /locus_tag="ACHK8E_001535"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP0343822.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(9491..11743)
                     /gene="hypF"
                     /locus_tag="ACHK8E_001536"
     CDS             complement(9491..11743)
                     /gene="hypF"
                     /locus_tag="ACHK8E_001536"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP0343823.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11896..12423)
                     /gene="hydN"
                     /locus_tag="ACHK8E_001537"
     CDS             complement(11896..12423)
                     /gene="hydN"
                     /locus_tag="ACHK8E_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP0343824.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12572..13582)
                     /gene="ascG"
                     /locus_tag="ACHK8E_001538"
     CDS             complement(12572..13582)
                     /gene="ascG"
                     /locus_tag="ACHK8E_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP0343825.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13842..15299
                     /gene="ascF"
                     /locus_tag="ACHK8E_001539"
     CDS             13842..15299
                     /gene="ascF"
                     /locus_tag="ACHK8E_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP0343826.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            15308..16732
                     /gene="ascB"
                     /locus_tag="ACHK8E_001540"
     CDS             15308..16732
                     /gene="ascB"
                     /locus_tag="ACHK8E_001540"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP0343827.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16890..17360)
                     /gene="hycI"
                     /locus_tag="ACHK8E_001541"
     CDS             complement(16890..17360)
                     /gene="hycI"
                     /locus_tag="ACHK8E_001541"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP0343828.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17353..17763)
                     /gene="hycH"
                     /locus_tag="ACHK8E_001542"
     CDS             complement(17353..17763)
                     /gene="hycH"
                     /locus_tag="ACHK8E_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP0343829.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17760..18527)
                     /gene="hycG"
                     /locus_tag="ACHK8E_001543"
     CDS             complement(17760..18527)
                     /gene="hycG"
                     /locus_tag="ACHK8E_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP0343830.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18527..19069)
                     /gene="hycF"
                     /locus_tag="ACHK8E_001544"
     CDS             complement(18527..19069)
                     /gene="hycF"
                     /locus_tag="ACHK8E_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP0343831.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19079..20788)
                     /gene="hycE"
                     /locus_tag="ACHK8E_001545"
     CDS             complement(19079..20788)
                     /gene="hycE"
                     /locus_tag="ACHK8E_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP0343832.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20806..21729)
                     /gene="hycD"
                     /locus_tag="ACHK8E_001546"
     CDS             complement(20806..21729)
                     /gene="hycD"
                     /locus_tag="ACHK8E_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP0343833.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21732..23558)
                     /gene="hycC"
                     /locus_tag="ACHK8E_001547"
     CDS             complement(21732..23558)
                     /gene="hycC"
                     /locus_tag="ACHK8E_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP0343834.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23555..24166)
                     /gene="hycB"
                     /locus_tag="ACHK8E_001548"
     CDS             complement(23555..24166)
                     /gene="hycB"
                     /locus_tag="ACHK8E_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP0343835.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24291..24752)
                     /gene="hycA"
                     /locus_tag="ACHK8E_001549"
     CDS             complement(24291..24752)
                     /gene="hycA"
                     /locus_tag="ACHK8E_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP0343836.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            24964..25314
                     /gene="hypA"
                     /locus_tag="ACHK8E_001550"
     CDS             24964..25314
                     /gene="hypA"
                     /locus_tag="ACHK8E_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0343837.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25318..26190
                     /gene="hypB"
                     /locus_tag="ACHK8E_001551"
     CDS             25318..26190
                     /gene="hypB"
                     /locus_tag="ACHK8E_001551"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP0343838.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26181..26453
                     /gene="hypC"
                     /locus_tag="ACHK8E_001552"
     CDS             26181..26453
                     /gene="hypC"
                     /locus_tag="ACHK8E_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP0343839.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26453..27574
                     /gene="hypD"
                     /locus_tag="ACHK8E_001553"
     CDS             26453..27574
                     /gene="hypD"
                     /locus_tag="ACHK8E_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP0343840.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27571..28581
                     /gene="hypE"
                     /locus_tag="ACHK8E_001554"
     CDS             27571..28581
                     /gene="hypE"
                     /locus_tag="ACHK8E_001554"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP0343841.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28655..30733
                     /gene="flhA"
                     /locus_tag="ACHK8E_001555"
     CDS             28655..30733
                     /gene="flhA"
                     /locus_tag="ACHK8E_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP0343842.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            30778..31206
                     /locus_tag="ACHK8E_001556"
     CDS             30778..31206
                     /locus_tag="ACHK8E_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFP0343843.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(31245..31589)
                     /gene="ygbA"
                     /locus_tag="ACHK8E_001557"
     CDS             complement(31245..31589)
                     /gene="ygbA"
                     /locus_tag="ACHK8E_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP0343844.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            31875..34436
                     /gene="mutS"
                     /locus_tag="ACHK8E_001558"
     CDS             31875..34436
                     /gene="mutS"
                     /locus_tag="ACHK8E_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP0343845.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            34542..35198
                     /gene="pphB"
                     /locus_tag="ACHK8E_001559"
     CDS             34542..35198
                     /gene="pphB"
                     /locus_tag="ACHK8E_001559"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0343846.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(35249..36016)
                     /gene="ygbI"
                     /locus_tag="ACHK8E_001560"
     CDS             complement(35249..36016)
                     /gene="ygbI"
                     /locus_tag="ACHK8E_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP0343847.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            36212..37120
                     /gene="ltnD"
                     /locus_tag="ACHK8E_001561"
     CDS             36212..37120
                     /gene="ltnD"
                     /locus_tag="ACHK8E_001561"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP0343848.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37117..38379
                     /gene="otnK"
                     /locus_tag="ACHK8E_001562"
     CDS             37117..38379
                     /gene="otnK"
                     /locus_tag="ACHK8E_001562"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP0343849.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            38376..39014
                     /gene="ygbL"
                     /locus_tag="ACHK8E_001563"
     CDS             38376..39014
                     /gene="ygbL"
                     /locus_tag="ACHK8E_001563"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0343850.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            39019..39795
                     /gene="ygbM"
                     /locus_tag="ACHK8E_001564"
     CDS             39019..39795
                     /gene="ygbM"
                     /locus_tag="ACHK8E_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP0343851.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            39884..41248
                     /gene="ygbN"
                     /locus_tag="ACHK8E_001565"
     CDS             39884..41248
                     /gene="ygbN"
                     /locus_tag="ACHK8E_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP0343852.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(41286..41522)
                     /locus_tag="ACHK8E_001566"
     CDS             complement(41286..41522)
                     /locus_tag="ACHK8E_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP0343853.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(41533..42960)
                     /locus_tag="ACHK8E_001567"
     CDS             complement(41533..42960)
                     /locus_tag="ACHK8E_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP0343854.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(42960..43553)
                     /locus_tag="ACHK8E_001568"
     CDS             complement(42960..43553)
                     /locus_tag="ACHK8E_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP0343855.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            43700..44107
                     /locus_tag="ACHK8E_001569"
     CDS             43700..44107
                     /locus_tag="ACHK8E_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0343856.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            complement(44228..45220)
                     /gene="rpoS"
                     /locus_tag="ACHK8E_001570"
     CDS             complement(44228..45220)
                     /gene="rpoS"
                     /locus_tag="ACHK8E_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP0343857.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(45283..46377)
                     /gene="nlpD"
                     /locus_tag="ACHK8E_001571"
     CDS             complement(45283..46377)
                     /gene="nlpD"
                     /locus_tag="ACHK8E_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP0343858.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(46562..47188)
                     /gene="pcm"
                     /locus_tag="ACHK8E_001572"
     CDS             complement(46562..47188)
                     /gene="pcm"
                     /locus_tag="ACHK8E_001572"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP0343859.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(47182..47943)
                     /gene="surE"
                     /locus_tag="ACHK8E_001573"
     CDS             complement(47182..47943)
                     /gene="surE"
                     /locus_tag="ACHK8E_001573"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP0343860.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(47924..48973)
                     /gene="truD"
                     /locus_tag="ACHK8E_001574"
     CDS             complement(47924..48973)
                     /gene="truD"
                     /locus_tag="ACHK8E_001574"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP0343861.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(48970..49449)
                     /gene="ispF"
                     /locus_tag="ACHK8E_001575"
     CDS             complement(48970..49449)
                     /gene="ispF"
                     /locus_tag="ACHK8E_001575"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP0343862.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(49449..50159)
                     /gene="ispD"
                     /locus_tag="ACHK8E_001576"
     CDS             complement(49449..50159)
                     /gene="ispD"
                     /locus_tag="ACHK8E_001576"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP0343863.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(50178..50489)
                     /gene="ftsB"
                     /locus_tag="ACHK8E_001577"
     CDS             complement(50178..50489)
                     /gene="ftsB"
                     /locus_tag="ACHK8E_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP0343864.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(50682..51005)
                     /gene="ygbE"
                     /locus_tag="ACHK8E_001578"
     CDS             complement(50682..51005)
                     /gene="ygbE"
                     /locus_tag="ACHK8E_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP0343865.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(51055..51660)
                     /gene="cysC"
                     /locus_tag="ACHK8E_001579"
     CDS             complement(51055..51660)
                     /gene="cysC"
                     /locus_tag="ACHK8E_001579"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP0343866.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(51660..53087)
                     /gene="cysN"
                     /locus_tag="ACHK8E_001580"
     CDS             complement(51660..53087)
                     /gene="cysN"
                     /locus_tag="ACHK8E_001580"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP0343867.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(53089..53997)
                     /gene="cysD"
                     /locus_tag="ACHK8E_001581"
     CDS             complement(53089..53997)
                     /gene="cysD"
                     /locus_tag="ACHK8E_001581"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP0343868.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            54249..55286
                     /gene="iap"
                     /locus_tag="ACHK8E_001582"
     CDS             54249..55286
                     /gene="iap"
                     /locus_tag="ACHK8E_001582"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP0343869.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   55307..55762
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=55429..55455
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
     gene            complement(55868..56152)
                     /gene="cas2e"
                     /locus_tag="ACHK8E_001583"
     CDS             complement(55868..56152)
                     /gene="cas2e"
                     /locus_tag="ACHK8E_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP0343870.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     gene            complement(56154..57071)
                     /gene="cas1e"
                     /locus_tag="ACHK8E_001584"
     CDS             complement(56154..57071)
                     /gene="cas1e"
                     /locus_tag="ACHK8E_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP0343871.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(57087..57686)
                     /gene="cas6e"
                     /locus_tag="ACHK8E_001585"
     CDS             complement(57087..57686)
                     /gene="cas6e"
                     /locus_tag="ACHK8E_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP0343872.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            complement(57673..58347)
                     /gene="cas5e"
                     /locus_tag="ACHK8E_001586"
     CDS             complement(57673..58347)
                     /gene="cas5e"
                     /locus_tag="ACHK8E_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP0343873.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            complement(58350..59441)
                     /gene="cas7e"
                     /locus_tag="ACHK8E_001587"
     CDS             complement(58350..59441)
                     /gene="cas7e"
                     /locus_tag="ACHK8E_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP0343874.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            complement(59454..59936)
                     /gene="casB"
                     /locus_tag="ACHK8E_001588"
                     /gene_synonym="cse2"
     CDS             complement(59454..59936)
                     /gene="casB"
                     /locus_tag="ACHK8E_001588"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP0343875.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(59929..61437)
                     /gene="casA"
                     /locus_tag="ACHK8E_001589"
     CDS             complement(59929..61437)
                     /gene="casA"
                     /locus_tag="ACHK8E_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP0343876.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
BASE COUNT        14716 a        16643 c        15844 g        14364 t
ORIGIN      
        1 tagattaggt tgccgtttgg taataaaaca ataaatcctg aaggagagaa caatgataga
       61 aaccattact catggtgcag agtggtttat cgggctgttc caaaagggcg gagaggtgtt
      121 taccgggatg gtgaccggca ttcttccgct gttgattagt ctgctggtta tcatgaacgc
      181 actgattaat tttatcggtc agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa
      241 ccctgtttcc cgttacctgc tgttaccgtg cattggcacg tttgtctttt gcaatccgat
      301 gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa cccagctact acgcggccgc
      361 ctcttatagc tgccactcaa tgaatggcct cttcccccat atcaacccag gcgaactgtt
      421 tgtttatctt ggcattgcca gcggtctgac aacactgaac ctgccacttg gcccactggc
      481 ggtgagttat ctgctggttg gtctggtcac caacttcttc cgcggctggg tgaccgatct
      541 gaccaccgcc atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcatctggc
      601 aggagcaaca tcatgacgcg tattcggatc gaaaaaggaa cgggtggctg gggcggcccg
      661 cttgagctgg aagccacgcc gggcaaaaaa atcgtctata tcaccgccgg tacccggcct
      721 gcgattgttg acaaactggc acagcttact ggctggcagg ctattgacgg atttaaagaa
      781 ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc
      841 tgcggcattt atccgaaacg gcgtattccc accattaata tccactcgac gggcaagtcc
      901 ggtccactag cgcaatacat tgtagaagat atttatgtct ctggcgtaaa agaagaaaac
      961 atcactgtag tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc
     1021 agcaagaaaa tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggtatgggt
     1081 tctgccgttg ccgtgttgtt tcaatctagt cgtgacacca tcgacactgt attaaaaacc
     1141 attctgccgt ttatggcatt cgtttcggcg cttatcggca tcattatggc ttccggtctt
     1201 ggcgactgga ttgcccacgg tcttgctccg ctggcgagcc atccactggg tctggtcatg
     1261 ctggcgctca tctgctcctt cccgctgctt tcacctttcc tcggcccagg cgcagttatc
     1321 gcacaggtta tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat
     1381 ctggctttac ctgcactgtt tgccatcaac gcgcaggcgg cctgcgactt catcccggtc
     1441 ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgttctg
     1501 gtgagccgct ttttaaccgg tgcgccaact gtactgatcg cctggtttgt ctccggtttt
     1561 atctatcaat agaggctgaa acatgaccgt tatttatcag accaccatca cccgtattgg
     1621 cgcgagtgcc actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc
     1681 agcggatctc gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca
     1741 tcccggtttg caattttcac tcgggcagca tcgctatccg gtgaccgccg ttggcagcgt
     1801 ggcggaagac aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga
     1861 agcggaattt ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg
     1921 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
     1981 cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
     2041 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
     2101 cgaatatggt gaaggtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt
     2161 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctacag
     2221 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
     2281 gctacaggtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat
     2341 ccgcgacggg attcaggggc gcattattca gatcaactcg aaatccggca aagtgggcag
     2401 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
     2461 ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaatctgct
     2521 gaaatcgccg atgtttcagt cgctattgcc acaatatgcg accaagctgg gtatcaaacc
     2581 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
     2641 ggatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcactggtca
     2701 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatta acagcggaga tccgttaagg
     2761 atctccgcga gactaataaa atgcctgatg cgctacgctc atcaggcata caggacttcc
     2821 gccactacat taaggaaaag ttatggtatc cgcactcatc accgtcgccg ttatcgcctg
     2881 gtgtgcgcaa ctggccttag gcggttggca aatttctcgt tttaaccgtg ccttcgacac
     2941 actatgccag caagggcggg ttggcgtggg ccgttccagc gggcgcttta aaccgcgggt
     3001 cgtggtcgcc atcgccctgg acgatcagca acgcatcgtc gacaccttgt ttatgaaagg
     3061 actgaccgtc ttcgcccgac cacaaaaaat tcccgcaatt accggtatgt atgcgggtga
     3121 tttacagccc gatgtgatct ttccccatga tccactatca cagaatgctc tatcattggc
     3181 gcttaaactg aaacgtggat aatttcgttg tgaatgttac ttgcttgcga agttatcatt
     3241 ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt cagcgtcagg ccgccattct
     3301 ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa ttggcgcaat actttgacac
     3361 cacaggcaca accattcgca aagatctggt cattctggaa catgccggaa ccgtcattcg
     3421 tacttatggc ggagtggtgt tgaataaaga ggaatccgat ccgccaatcg atcataaaac
     3481 actcatcaac acccacaaga aagagctgat tgcagaagct gccgttagtt ttatccatga
     3541 tggcgattcg atcattcttg atgctggcag taccgttttg cagatggtcc ccctgctctc
     3601 gcgctttaat aacatcacag tgatgaccaa cagcctgcat atcgtcaatg cgctatccga
     3661 actggataac gaacaaacta tcctgatgcc aggcggaaca tttcgtaaaa aatcggcctc
     3721 atttcacggg caactggcag agaatgcctt cgagcatttc accttcgata aattgtttat
     3781 gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg ttcaacgagg tttataccgt
     3841 cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg atggcggact catcaaagtt
     3901 tggccgtaaa agccccaacg tagtttgcag tcttgaaagc gtcgataagc taattaccga
     3961 cgcaggtatc gatccagcgt ttcgtcaggc gctggaaaag aaagggatcg acgtgatcat
     4021 aaccggagag agcaatgagt gaagcactac tgaacgcggg acgtcagacg ttaatgctgg
     4081 agttgcagga agcaagccgt ttaccggaac gtctgggcga tgattttgtt cgcgccgcca
     4141 atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg aattggcaaa tcgggccaca
     4201 ttggtaagaa aatcgccgca acgcttgcca gcaccggcac tcctgccttt tttgtccatc
     4261 cggcagaggc attgcacggc gacctgggga tgatcgaaag ccgcgatgtg atgctgttta
     4321 tctcttactc cggtggcgcg aaggaactgg atctgattat tccgcgtctg gaagataaat
     4381 ctatcgcgct gctggcgatg accggcaaac cgacgtcacc gctgggcctg gcggcgaaag
     4441 cggtgctgga tatctccgta gaacgcgaag cctgcccgat gcaccttgcg ccgacctcca
     4501 gcaccgtcaa taccctgatg atgggtgacg cgctggcgat ggcggtcatg caggcgcgcg
     4561 gatttaatga agaagatttt gcccgctccc accctgctgg agcactgggc gctcgcttgc
     4621 tgaataaagt gcatcatctg atgcgccgtg acgatgccat cccgcaggtg gcgttaaccg
     4681 ccagcgtgat ggacgcgatg ctggaactta gccgcaccgg tttaggtctg gtggcagtgt
     4741 gcgatgatca acgactggtg aaaggcgtct ttaccgacgg tgatttacgt cgctggctgg
     4801 ttggcggcgg cgcactcacc acgccagtca atgaagcgat gacggtcggc ggcaccacgt
     4861 tgcaatcgca aagccgtgcc atcgacgcca aagaaatcct gatgaaacgc aaaatcaccg
     4921 ccgcaccggt agtggatgaa aacggcaaac tcaccggcgc aattaacctg caggatttct
     4981 atcaggccgg gattatttaa tccttcagcc ccagacgttt cgccagccga tgcaggttgg
     5041 cgacgtcggt ttccagcatc cgcgcacagg cggcccagtt gtgatgattt tgcgccagtg
     5101 cctgacgaat ggtttcacgc tggaacgctt ctgtcgcttc acgcaggttt tgcttaacga
     5161 taggcaccgc cgccgcttct ggcggcggca acgtcacctc aggaaaagca aaatgttgcg
     5221 cctcaagaat cacttcatcg ccgctgcgag tggctctcga cagtactacc gcccgatgaa
     5281 tagcatgttc cagttcgcgc acgtttcccg gaaaactgta gtgttgcagt aaatttcgcg
     5341 ccccggcact taataccacg cgggagagcc ccagccgcaa acgacactgc tcgcagaaat
     5401 accccgccag cagaatgaca tcatcgcccc gctcacgcag cggcggcacc gaaagtggaa
     5461 acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc tgccagcacc tcttcgcgta
     5521 aatcgcggtt agtcgccgcc agcacgcgca catcgacccg caaactgcgg tcatcgccaa
     5581 cgcgctgaat atcgccatac tgcaacaccc tcagcagctt ggcctgcaat gccaacgaca
     5641 actcgccgat ctcatcgaga aacagcgtgc cgttatccgc catttcaaac ttcccgctgc
     5701 ggttactgat agcaccagta aacgcccctt tcacatgccc gaacaactca ctttccgcca
     5761 cactttccgg cagtgcggca cagttgagat agaccagcgg attcaccgcc cgtggcgagg
     5821 cttcatgaat cgctttcgcc accagctcct taccggttcc cgtctcaccg ctaattaaaa
     5881 cgttgagatc ggatgccgcc acaatctcaa tctctttttt caattgcgtc atgcctgggg
     5941 acaaaccaat catctgcgtc tgtttcaccg cttcaaacgg cgcggcatcg cctggcagca
     6001 tattctggct ttccagttgt tcaatcagta acgcattact taacgctccc gccgccagtg
     6061 cggcaatcag ccgtagctct tcgtcgctga aaacatcgaa ctgatcgggc tgcatcccgt
     6121 cgagcgtcaa ggcaccgatc aggttttgcc cggcaaacaa cggcaaacca acgcaggcgt
     6181 gaaccttcag actctcctgc cctggaatca aaccgtcata aggatcgggc aattcgctgt
     6241 ctgcgggaaa gcgcaccaca tcacccgcgc gggcaatcgc ttccagccgt ggatgacctt
     6301 ccagcgcaaa gcgtctaccg agtacatcct tcgccagccc gtcgatggca agcggaataa
     6361 actgccgcga gtcgtaacgt agcaacgcag acgcatcgca ctccagcacc tgacgcagcg
     6421 tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc acgctgcaat tcgatggcga
     6481 tattcgccag cacatcaacg gaaaaactca tctttgcctc actgtcaatt tgactataga
     6541 tattgtcata tcgaccattt tattgatagt cattttgact actcattaat gagcacaatt
     6601 ttatttatag agtaaaaaca attagataaa aaactggcac gcaatctgca atcagcaaga
     6661 catcttttta gaacacgctg aataaattga ggttgctatg tctattgtgg tgaaaaataa
     6721 cattcattgg gttggtcaac gtgactggga agtgcgtgat tttcacggta cagaatataa
     6781 aacgctgcgc ggcagcagct acaacagcta cctcatccgc gaagaaaaaa acgtgctgat
     6841 cgacaccgtc gaccataaat tcagccgcga atttgtgcag aacctgcgta atgaaatcga
     6901 tctgacggat atcgattaca tcgtaattaa ccatgctgaa gaggaccacg ccggggcgct
     6961 gaccgaactg atggcacaaa ttcccgatac gccgatctac tgtactgcca acgctatcga
     7021 ctcgataaat ggtcatcacc atcatccgga gtggaatttt aatgtggtga aaacaggcga
     7081 cacgctggat atcggcaacg gcaaacagct catttttgtc gaaacgccaa tgttgcactg
     7141 gccggacagc atgatgactt acctgacagg cgacgcggta ctgttcagta acgatgcttt
     7201 cggtcagcac tactgcgacg agcatctgtt caacgatgaa gtggatcaga cggagctttt
     7261 cgagcagtgc cagcgttatt acgccaatat cctgacgccg ttcagccgcc tggtaacgcc
     7321 gaaaattacc gagatcctgg gctttaactt accagtcgat atgatagcga cctctcacgg
     7381 cgtggtatgg cgcgataacc cgacgcaaat tgtcgagctg tacctgaaat gggcggcgga
     7441 ttatcaggaa gacagaatca ccattttcta cgacaccatg tcgaataata cccgcatgat
     7501 ggccgatgct atagcccagg gtattgcgga aaccgaccca cgcgtagcgg tgaaaatttt
     7561 caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat gtcttccgct caaaaggcgt
     7621 gctggtcggc acttcgacga tgaataacgt gatgatgcca aaaatcgccg ggctggtgga
     7681 ggagatgact ggtttacgct ttcgtaacaa acgcgccagt gctttcggct ctcacggctg
     7741 gagcggcggt gcggtggatc gtctttccac acgcctgcag gatgcgggtt tcgaaatgtc
     7801 gcttagccta aaagccaaat ggcgaccgga ccaggacgct ctggagctat gtcgtgaaca
     7861 cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg ccgcagagca cggtgaatac
     7921 ggtggttaaa gaagaaacct ctgccaccac gacggctgac ctcggcccac ggatgcagtg
     7981 cagcgtctgc cagtggattt acgatccggc aaaaggcgag ccaatgcagg acgttgcgcc
     8041 aggaacgccg tggagtgaag ttccggataa cttcctgtgc ccggaatgct cccttggcaa
     8101 agacgtcttt gacgaactgg catcggaggc aaaatgagca acggcattgt gatcattggt
     8161 tcgggcttcg ccgcccgcca actggtgaaa aatattcgca aacaggacgc ctgtattcca
     8221 ttaaccctga ttgccgccga cagcatggat gagtacaaca aaccggacct cagccatgtt
     8281 atcagccaga ggcaacgtgc cgatgacctt acccgccaga cggcgggtga atttgccgag
     8341 cagtttaatc tgcacctgtt tccgcacacc tgggtaacgg atatcgatgc cgaagcccat
     8401 gtggtgaaaa gtcagaataa tcagtggcaa tacgacaagt tagtgctggc aaccggtgcc
     8461 agcgcctttg tcccgccagt gcccgggcgt gagttaatgc tgacgttaaa tagtcagcaa
     8521 gagtatcgcg cctgtgaaac gcaactgcgg aatgcccgac gcgtgttgat tgttggcggt
     8581 ggcttgattg gtagcgaact ggcgatggat ttttgtcgtg caggcaaagc ggtcacgcta
     8641 atcgacaacg ctgccagtat tctggcagcg ttaatgccac cggaagtaag cagccgcttg
     8701 cagcatcggt tgacggagat gggcgttcat ctgctgttaa aatctcagtt gcaggggctg
     8761 gaaaaaacgg attctggcat tctggcaacg ctggaccgcc agcgcagcat cgaagtggat
     8821 gcggtaattg ccgccaccgg actgcgcccg gaaaccgccc tggcacgacg cgccgggctg
     8881 acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa ccagtaatgc cgatatttat
     8941 gcgctgggcg attgcgcgga aattaacggt caggtattgc cgttcctcca gccgattcaa
     9001 cttagcgcga tggtgctggc aaaaaatctt ctcggcaata acacgccgct gaaactcccg
     9061 gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc atctggcagg cgaaacccag
     9121 cgtcaggatt tacgctggca aattaatacc gaacgccagg gaatggtggc gcgcggtgtt
     9181 gatgatgccg accagcttcg cgcctttgtg gtcagtgagg atcggatgaa agaggcgttt
     9241 ggattgttga aaacgttgcc ggtgtaggcg agctacagtg ccccaaatgt cggatgcggc
     9301 gctgacgcgt cttatccgac ctacagggaa cgcatgtgtt gatcacataa tacgtttatg
     9361 tcgcatcctg cactgtccca aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac
     9421 ggggacgcac gtgtaggccg gataaggcgt ttacgccgca tccggcaatg gtgtccaaat
     9481 gcaacacgct ttatccgttc tggacttcac ccgctaacca gcgcgccgca gcaataaccc
     9541 cctgccccag agacaatcca ccatcgcccg ccggtaaact ctgcgggaag agcaacgtaa
     9601 aatcagcaag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata actccgccgc
     9661 taaataccag cgtagtgata ccccgcatcg tggcctgctc acgcatcaac gcggcaaaac
     9721 cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aaccggagcc tgccagctca
     9781 gccactgctg ccagaaagtg gcgagatcca gttgattgtc caccagcggc atcgtcaccg
     9841 gatgcgtcac tccgtcgcac gaagctgcga gcgcctccag agcacaagcc gcttcacctt
     9901 cataacttaa cgtggctggc gcacagccca gtcccgccgc cacagcatcg aacaaacgcc
     9961 cacacgatga cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agcacgctcc
    10021 agttttgctg ttgcacactt gccgtttcag ggtaattctg ccactccggc acaaagcgca
    10081 ggcactgcgc cagcaggtta cgccacggct gcttcgccgc caaatcgccc ccagcaagcg
    10141 ccactgcagg caagccgccc aggtgctggc attcgcgata gttcacccgc aggcactcgc
    10201 cgccccacaa agcgccgttc tcccccatac cgataccgtc gagcgtcaac gcaatgacat
    10261 caccgccatc cagtggccag tgatgttctg ccagacacgc cgccgcatga gcatgatgat
    10321 gcagcaccgt ttgcgtcggc agattcatct cacgcgccca ctggctggag acatagcccg
    10381 gatgcgcgtc atgcacaacg tattgcgggg tgaaatcgta gatgttttgc atcaggcgta
    10441 acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctggctca
    10501 acaccgcttg ttcaccgcgc accaggcaga aggtattttt cagatccgcg ccgagacaca
    10561 gcacaggcgg aacattttta aagcccggag gcaaagccag cgcatccggc acataccccc
    10621 gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga atcatccatc cgctgcacga
    10681 tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt
    10741 cgttgctgat agccggtggt ttaccgctca ggttgccgga ggtcatcacc agcgggcatt
    10801 gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacatcacc ccaacttcgt
    10861 taaggccagg ggcgatatca tcacaaagct caggaacgta ttttttatcc accagcacaa
    10921 tcggcgcggc gggtgtggta agcaactgac gggcagcgtc tggtaaacct tctgccactg
    10981 gcaacataac cgccagcggt ttcgccgggc gatgtttgcg cgcccgcagt gtcgccaccg
    11041 cgttactgtt acgcgcatcg caggcgagat gaaatccgcc aatccctttg atggcgacaa
    11101 tgttgcccat ttttaactgt gcgatagctg cctgtaatgc cgcctcttgt tctgcatgtt
    11161 caccatgact tacccattca agatgggggc cacactccgg gcaggccacc ggctgggcgt
    11221 ggaagcgacg atcgagcggg tcacggtact ctttgtcaca ggccggacac agcggaaacg
    11281 ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg aaacgcggac
    11341 cacagtgagt acagttgata aacggataac gccagcgtcg ttcgcctggg gtattcattt
    11401 cggcaagaca agcagggcaa gtcgcggcat ccgggacaat ttgcgtattc atgacgccac
    11461 ccgcgctctg gcggatggtg aactcagtgg gcagttgtga ccagataaac ggctcacgct
    11521 cgacgctatc aatacgcgcc agtggcgggc agtgctgatg caactgcaca agaaacgttt
    11581 ccggatcttc cagcagccgg acttctacgc catcgccgtc attacagaca tcgccgtgaa
    11641 gatttaattg ctgtgccagt tgccagacaa acggacgaaa accgacgccc tgcactttgc
    11701 cacgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat
    11761 catgaattgc gtaacccgcc cagccggaca cgacagcgtc gcatccggca gtcacaggtc
    11821 ggcgatactg cccgcttcgt attctacgaa tatttccggg aattcctttg atgccagaac
    11881 agttcagtaa gatttttaga acatcagcgc cgtacggcga cgtttttctg cgctcagttg
    11941 ttcaagttta ttacgatcga cacaaatcag cgcatgggtc gggcaagccg ccatacacgc
    12001 cgggccgtct tcacgatggt tgcacaggtc gcatttattg gcttcggctt tgtcagcccg
    12061 tacattcagc cccgcgccgc tgttgcgaat caccggacgt accaccactt ccatcgcgcc
    12121 atacgggcaa gccacaacgc aggttttgca accaatacaa cgttcctgca tcacatgaac
    12181 aaacccttta tcacgactga tagcaccatt cggacagacg ttagcgcacg gtgcatcttc
    12241 acactgacgg caaactgtcg ccgtggaaat gttcacacct ttaatgacat ggattcgcgg
    12301 taaaaaagtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag aaaccacgca
    12361 cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt
    12421 catcaaattc tccagcaatg acagttaatg cgccgataca ttcacaaatc atgccagttt
    12481 ttaatttact gttatttaaa gaaattaaat tctgtaatgc aggaaaaacg atgtcatcga
    12541 cactagtgac gatgacatgt gatgacaatg tttatcgcga aagagcaatg agggagtcgc
    12601 ggcggatcag ttttccgctg aaggttttcg gcggtgtgta agcaccacca tcgagcataa
    12661 atatcagccg cccaattatt tcctgaatca tctcagtcac cggaattttt acgctggaga
    12721 gcgccggaac ggtgtagggg gcaatagcga tatcatcgaa tccgataact gacacctgct
    12781 ctggcaccgc tacgccgcgc tcgtgtaacg ctttcatcgc acctatcgcc atatcgtcgt
    12841 tactggcaac taacgcgcta aatttagccc cacgttcgag caacatttct accccttcgg
    12901 ccccgctggc aggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat
    12961 gctgcgccag cgcgtcttta tacccggcaa gacgttcaac gctggtgggg gaatccatcg
    13021 aaccggtaag gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcgttaa
    13081 agctggtctg tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat
    13141 taagcaccat tatcggctga ctgtgtgcgt caatgatgtc atcaatctca tccacgctta
    13201 aaaaacgcgg gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct
    13261 ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgtcgc cctttctctt
    13321 ccgccattcg cgcggcatga aagagtaatt cactaaaata aatgccgtgg taaagcgtgt
    13381 tggtcacgac cagccccagc gtctgagtac tcttcgccga cagattgcgc gccagcaagt
    13441 ttggacggta accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga
    13501 cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg
    13561 ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact
    13621 gcaccgtttg ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc
    13681 acaaatataa aaaatgaaca attaactctc ttgcttattc agtgacaact attcatgatt
    13741 ttgtgaaacc ggtttcctaa ttccgtttca gcatcggcat ttttccgtca cgtcgactga
    13801 taacaactac atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg
    13861 cgctggcacg ctcggtgata gcggcactgg gcggcgttga taacatctcg gcagtcacgc
    13921 actgtatgac gcgtttgcgc tttgttatca aagatgacca gcttatcgac agtccgacgt
    13981 tgaaaaccat ctccggcgtg ctcggcgtgg tacgtagtga caaccagtgt caggtgatta
    14041 tcggcaatac cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc
    14101 agcccgcaca gcccgtgggt aaacccaaac ttacgctacg tcgcataggt gcggggatcc
    14161 tcgatgcgct gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg
    14221 tcaaactgct ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct
    14281 taaccattct gaatgtgatt ggtgacggtg ctttcttctt cctgccgctg atggtcgcgg
    14341 cttcggccgc catcaaattt aaaaccaata tgtcgctggc gattgccatc gcgggtgttc
    14401 tggtacatcc gagttttatt gaactgatgg cgaaagcggc ccagggtgaa catgtcgaat
    14461 ttgccctgat cccggtcacc gcggtgaaat acacctacac ggtgatcccg gcgctggtga
    14521 tgacctggtg cctgtcatat atcgaacgct gggtggatcg cattacaccg gcggtgacta
    14581 aaaacttcct caagccaatg ctgattgtgt tgattgccgc accgctggca atcctgctga
    14641 ttggcccgat tggtatctgg atcggtagcg ccatttcggc gctggtttac accattcatg
    14701 gttatctggg ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga
    14761 ccgggatgca ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag
    14821 aagggatggt catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg
    14881 cagtggcgtg gaaaacgaaa aacccggaac tgcgtcagac ggcgctggct gcggcggcat
    14941 cagccattat ggcggggatt tccgaaccgg cgttatatgg cgtggcgatc cgcctgaaac
    15001 gtccgcttat cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg
    15061 ggcttgccag ccactcaatg gcagcgccgg ggctatttac cagcgtgcag ttcttcgatc
    15121 cggcgaatcc aatgagcatc gtctgggtgt tcgccgtcat ggcgctggcg gtcgtgctgt
    15181 cgttcatcct cacactgttg ctcggctttg aggatattcc tgttgaggaa gcgactgccg
    15241 aggcgcgaaa gcatcagagc gcacaaccga ccgtcgccaa agaagtaagt cttaattgag
    15301 gatgaaaatg tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca
    15361 gtctgaaggt gcgtaccggg aaggtggcaa agggctgacc accgtcgata tgatcccaca
    15421 cggcgagcat cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga
    15481 cgagttttat cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc
    15541 cctgatggca gagatgggat tcaaagtgtt ccgtacctca attgcctgga gccgcctctt
    15601 cccgcagggc gatgaactga cgcccaacca gcagggcatt gctttttacc gttctgtctt
    15661 tgaagagtgt aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc
    15721 gatgcatctg gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag
    15781 ccgctacgcc cggacctgct ttgaagcatt tgatggtctg gtgaaatact ggctcacctt
    15841 caatgaaatc aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga
    15901 aggcgaaaat caggatcagg tgaaatatca ggctgcgcat caccaactga ttgccagtgc
    15961 attagccacc aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc
    16021 gggcggtaac ttctaccctt acagttgcaa gccggaagat gtctgggcgg cgctggagaa
    16081 agatcgggaa aacctgtttt ttatcgatgt gcaggcgcgg ggcgcgtatc cggcttactc
    16141 tgcccgcgta ttccgcgaaa aaggggtaac catcgacaaa gcaccgggcg atgatgagat
    16201 cctgaaaaac accgtcgatt ttgtctcttt cagctattac gcctcacgct gcgcctcggc
    16261 ggagatgaac gccaacaaca gcagtgcggc gaacgtggtg aaatcgctgc gtaacccata
    16321 tctacaggta agcgactggg gctgggggat tgatccactc ggtctgcgta tcaccatgaa
    16381 catgatgtat gaccgctatc agaagccgct gtttctggtg gaaaacggcc tcggcgcaaa
    16441 agatgaactt gctgccaatg gcgagattaa cgatgactat cgcatcagct atttacgcga
    16501 acatatccgc gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac
    16561 ttggggctgt attgatttgg tttccgcctc aacgggtgaa atgagcaaac gctacggttt
    16621 tgtctacgta gaccgtgacg acgcaggcaa cggtacgctg gcgcgcacgc gtaagaaatc
    16681 gttctggtgg tataaaaaag tgattgccag taatggtgag gatttagagt aagtaacagt
    16741 gccggatgcg gcgtgaacgc cttatccggc ctacatgtcg cacgaaccag gtaggcccga
    16801 taagcgcagc gcatcgggca atctggcgtt tgctatcagt tctgattccc tcatcaagaa
    16861 catccctgtc ctgattcctt actgaaaaac tactcttctt ccaccgctaa ctgcgcgaat
    16921 ccgccattcc cttcccagcc ttccagtcgt tgataaacgg tttctaccgc atctttaatc
    16981 ggctgggtca tcgggtagta aaagccgacg atatccggct ggatgccgag gaaaatcact
    17041 tcgccaatgt cttcttttaa ctggtcgata aggtagttca acggcatgtt atgggtagtc
    17101 atcataaaca tctcggcgat atcatccgga tcgatgatgc ggatctcgcc ggggtttaac
    17161 cccatatccg tggcgtcgac aatcagcagc cgtgtcgggc gcagttcgcg gatggcgacg
    17221 atgtcgtttt ccggagcgct accgccgtca atcaccaccc agttaccttt cggcgcggcg
    17281 gcgcactttt ccgccagcag cgggcctgcg ccatcatcgc ccatcatgct attgccaaca
    17341 caaagtaaaa cgtcagtcac gcagtctcct caccatcaaa tagatggcgt tttcctgatg
    17401 aatatcatgc agcatactta acagcattgt actccattct ttctgctgcg aagtttgcgc
    17461 tgcccgggct ttatcgaatg cgttagccag catcggcaca tggttaatgt cgatgacgat
    17521 ctcaccatat ttcggcacgc cttccatttt gcggcgggct tcgctgcccg cctccagagt
    17581 ggcaatccat gccagatagt catcccacgg gcaggtaagc gccgcttcca ggcaatcgat
    17641 aaccccaagg tggtgaccaa tcgccaggct gtaatagacc acctgctgcg cctcggcggg
    17701 cgtagcatcg ttctcatcaa taaatttacg gctcagttga ctgaacacca ccttttcact
    17761 catcggatac gcgcctcttc aacaacatga ttcagatggc tgacaatctc gttcagacgc
    17821 ggatcgtttt ccgcttccag ccagcgtgca acctgttctt cgccctgccc taactgtgta
    17881 aggtaatcat cggcaatctg acgaccgtaa cgataacccg ccaggcggcg tgcttcgcga
    17941 tccactttca cacgcagcgg ctgcaccata tcgccatgca ggatctccgc cggttgttca
    18001 tcctgctcac ccggcccacg ggcgtgaatt ttctgctcca gcaggccgag cgccattgca
    18061 aagccgtata gcgtagcggc aggcgttggc gggcagccag ggatgtagac atctaccggg
    18121 acgattttat ccgtaccgcc ccacacgcag tagagatcgt ggaagatccc gccactgtta
    18181 ccgcaggcac cgtaggagat acagattttc gggtccggcg cggactgcca cgcacgcagc
    18241 gcaggggatc gcattgcacg ggtgaccgcg ccggtaaaca gtaaaatatc cgcatgacgc
    18301 ggtgaaggaa cgactttaat gccgaagcgt tctgcatcaa acagcggcga aagcgtggcg
    18361 aaaatttcga tttcgcagcc gttgcagcca ccgcagtcca cgcggtaaac gtaggcagaa
    18421 cgtttgattt ttttcagtaa cgacgccttc atgctggcga tggattcatc caccgtcatc
    18481 gggaccggaa tgccgttggc gtcacggggg cctaataaat tgctcatcag atggcctctt
    18541 tcatatggcg agtcagttca atacggtcgg acggcaccag gcatttctgg cgcttacatt
    18601 ccgggcaagt ctcaaagctt tcgcggtggt tttccgcgcg gctgtcacca ttgtgcttaa
    18661 gcagcgcaat ggcgtagtcg atctctttct ggacggcgaa aggacgattg cagacgcggc
    18721 agttgcacag cgcgaagcgg gactgttgga ggaagtcttc tttcttccac accgccagtt
    18781 cgtactcttg cgacagtttg atcgccgccg tcgggcaaac ttcttcgcag cgtccacaga
    18841 agatgcagcg cccgagatta aactcccagg ccagctctcc tgtggcgagg tcggtttcaa
    18901 ccgttaaggc gtttgacggg caggcattga cgcaggccgc gcagccgatg cactgctgcg
    18961 ggttctgctc tggcttacca cggaagtttt tatcaaccgc aatcggctcc agcggataag
    19021 acgaggtcgc cgtgccggtt ttgatgactt ttttgataaa ggtaaacatg gcgattcctt
    19081 atttcagcgg cgagttttta cgctcaatgc tgtaacgctc gagttctttg tacggcacca
    19141 ctttgctctt cttcttacgc acatcgacca cggtcatgcg gtcggtacag gagtagcaag
    19201 ggtccaggct accgataatc agcggcgcat cggaaacggt gttgccgcgc agcatgtagc
    19261 gcagggtcgg ccagttcgcg taggtcgcgg cacggcagcg ccagcggtac agcttctggt
    19321 tgtcgccggt catgctccag tggatatcgt cgccgcgcgg cgcttcggca aagcccagcg
    19381 caaaacggtg cggaatgtag gtaaaacctt ctaccatcaa cggaccgccc ggcaggttat
    19441 ccagaccgta gtcgatcatg ttcagcgcgg tatagacctc gttgattcgc actttcagac
    19501 gggaaataac gtcgcagccc tgctcgctgt ggacttccat tggcagcagg ccataaccga
    19561 caaacgggtg atcggcgcga gtatcgcggg cgtgaccgct ggcgcgaacc atcgggccaa
    19621 cgttactgaa atcgcgggcg atttccgggt ccagacgacc aatgccgacg gtgcgctgtt
    19681 ccatgttcgg tgtgctcagc agcacatcca ccagctcctg cacttcacga cgcatctgtt
    19741 gtgccagctg gcgggtctgg atcatgtcgt ctttcagcag atcgcgacga atcccgccga
    19801 tcaggttcag gccgtaggtt ttacgtgcac cggtaaggat ctctgccatt ttcatggagg
    19861 tttcacgcac gcggaagaac tgcataaagc cggagtcgaa gccggtaaag tgacaggcga
    19921 ggccgagatt gagcagatgc gagtgcaggc gttccacctc caacagaatg gcgcggatca
    19981 tctgcgcacg ttctggcacc tgaataccca tcgcgttttc caccgacgtg gtgtaggcgg
    20041 tgctgtgagc aaagccgcag atcccgcaca cacggtcaga gaggaaggtc acttcgttgt
    20101 aacccatacg ggtttccgcc agtttttcca tgccgcgatg gacgtagaac aggcggtagt
    20161 cggcgtcgat aatgttttca ccatcgacaa acagacggaa gtgacccggt tcgtcagaag
    20221 tgacgtgcag gggaccaatc ggcacgacgt tgtttttctt gtcgcccagt tcgttgatga
    20281 actcgtaggt ttcagcatcg gtagtcggtg ccggacgctg acgataatcc atgctgtctt
    20341 tacgcagagg ataaagttca tccggccagt catccggcag caccagacgg cgttcatccg
    20401 gcagaccaac cggaatcaaa ccgtacatat cgcgcacttc acgctcgccc cacactgccg
    20461 ccggaacgcg cggcgtcacg gaaggatact ccggtttgtt ggcgtcaact tcgacgcgca
    20521 ccgttaccca acacttagtc cccttctcca tcgacagcac gtaataaacg gcgtaatgac
    20581 cattcagttt gcgttcgtcg ttaccaaaca gcaccgacag ccagcccccc tgcttgtagt
    20641 agagaaactc caccacttcc ggcaggtagt tcaccttcac ggtcacggtc agctgatctt
    20701 tggtctgcca ggcgtggtcc agcacgacgc ccggaaatgc ctcattcagc gcggcgagat
    20761 aatgttgacc taatttttct tcagacatgc tcaaactctc tttaatcacg ccgccagcaa
    20821 ggagacgaac gctaaaaatg caaagccaaa accagcccag gtaatgcgtg gagtaatatc
    20881 aagacgcaaa cgggccatgc tgttttcgaa cagcgcgata accagtacgc cgaccaccag
    20941 tttgacgatg gcaatcacca gcgccagcag cagcccaccg acggtgaagg tttccatttg
    21001 tccccacgga ataaacaccc cgacgaacat ctgcaacacc accagctgtt tcaggctgat
    21061 gccccatttc atgacgccaa agccgctgcc gctatactca gagagcggac cttcctgcaa
    21121 ctcctgctcg gcttccgcca ggtcgaacgg cagtttgccc atttcgataa aagtggcgaa
    21181 cgcacaggca caaagcgcca gcaccagcgg gatgctctgg ctcagtggcc agtgataaac
    21241 ggtgtcggtg atgttgctga tgttggtgga accggcaacc tgtgcggcga cccacagacc
    21301 aagcagcagc atcggttcaa ccaacacgcc aagcatcgct tcacggctcg cgccgatagc
    21361 ggtaaacggg ctaccagtat ccagaccaga aatggcaaag aagaaacgcg cgatggcgaa
    21421 gagatacagt aaggtgatca aatcgcccag ttgcggcagc ggagagccga cggtcaccac
    21481 cggcagcgca gtggcgatag tcagcatgac gcccaccatc acatacggcg tcaggcggaa
    21541 cacccagccg gaggcatccg ggccaacgct ctgacgcccc agcagtttga taatgtcgcg
    21601 atactcctgc aacacgcccg gcccacgacg gttatgcaag cgggcgcgcg ctacgcgggt
    21661 aataccggag agcagcggcg caacggcaaa taacaccagc gcctgaatta acggatataa
    21721 aacactcatt ctcaggctcc tcgtgaaaca ataatcacca ccagtaccgc cagttcaacc
    21781 agcgccatcc ggcggaacag caacgcactc ccctcgcact gccagcccgg caccagcgac
    21841 accggattca gccatttgcg tagtttcagc accggcgcaa acgcctgttt caccggcatg
    21901 gcaaaaccgt gagcggtaat caccattgat ttttcatgat cgtagccgca cacccaggcc
    21961 gcgccgcggg aacgcgacgg caaacgatcg cctttgcaaa tcgccataat gatgaatggc
    22021 agcagcgggc aggcaatcag cagcaacgtg atcatgggtt gagaaacggt ggtgttagca
    22081 ggctccagcg gcagaggtac agcagcagag agcatcggca gtagccacgg cgcagcaaca
    22141 ccgccaatta cgcagcaaat cgccagtgcc actacgctta cgctcatcag gagcggcgca
    22201 caggtggcgt tttcggcctc tttggtacgc ggcgcgccga ggaaagtaac gccataaact
    22261 ttcgccatac acatcaccgc cagcgcaccg gtaattgcca gccccacggc gagcagcggc
    22321 ccgagaagac gggcaacaaa cgcgccacta ttgctcagtt tgaagaagga ttgatagata
    22381 acccattccc cggcaaaccc gttcagcggc ggcagcgcag ccattgccat cagcccgact
    22441 aacatggcga tggagataac cggcattttc ttgccaatac caccgagttt ttcgatatcg
    22501 cgatgaccgg tacggaacca gacgctcccc gcaccaagga acagtacgct cttgaacagg
    22561 ctatggttaa gcagatggta cagaccacca accagaccaa gggcaatcag cgccggttgt
    22621 tcgagcgcga tacccgttac gccagcgccc agccccagca ggatgatgcc gatattttcc
    22681 agggtgtggt aagccagcag acgttggata ttatgttcca tcaacgcata cagaccgccg
    22741 acgaatgcgg tgatcatgcc gagtaccagc agtgcgatcc cccaccacag cggtgcatta
    22801 ccgcccagta gtgacagagt taaaatgccc agcaggccaa ttttcatgac taccgtggaa
    22861 aacagcgcgg cagctggcgc agaggcgttg gcatgtgcct gtggcaccca gccgtgcagc
    22921 ggaataatcc cggccagcag gccaaagcca atcacaccga gcagccagat atcggaaccg
    22981 agcggcagct gttgcatacg catatccagc aggcgcagat ccagcgtgcc gtaacgctgc
    23041 cacagcagcc agcaggcaat cgccagcagc agagtgccaa gacgccccag cgcaaaccac
    23101 agtttgccct ctttgctgtt gctggtgagg aacaccgcac acagggccat gatttcggcc
    23161 attaccacga acatgccgag gttgctggcg atgacggcgc agacggcggc agccatcaac
    23221 atattgatct gcaagccgtt gcacttcacc tgcgcgtggc gatgccagtc aatgttgtag
    23281 aggctgacaa acagaccgca taggccgagc gtaatcagcc aaatcgcatt aagcggagag
    23341 atttgcacat cgtagcttac cagcgacagc gcaccgctca cgccaaccgc gccagtcagc
    23401 actgtgaagc ccgcggctgc cgtatacagg ctaccaaccg cgccgccaat tccggctatc
    23461 cagccgctta gcgccttttg aaaagaaaag agaaatgcca gaacagcggc ggcgacaaac
    23521 cacgccacgc cgctattgat cagggaaatt gcgctcattt agcctctcca ctttgagcct
    23581 gctgaaacaa ggtgagatcg ccaaaatccg tgttaaaagt cagctcacgc ttacgtttgc
    23641 tgacgcgggc gatatcggtg ttatccacca gatgcagggc tttagtcggg cacatccgca
    23701 cgcaggccgg accttgttca tcaaagctac aaaggtcaca tttgacggcg atcgcgcgaa
    23761 tacctggcac ccagtcaagc aatgtgctga cacgcgccgg agcaggcggt gccggtggcg
    23821 ctttcggggt attggcgttt gccggaatat ccagcggacg gctgccggaa aattcaattg
    23881 cgccaaacgg gcaggcgatg ccgcacagtt tgcagcttac gcacagactt tcattcaact
    23941 gcacggcccc atcaacgcgg gtgatggcgt taaccgggca aaccaccgca cagggcgcat
    24001 cttcacagtg gtgacagagc tgcggcgcag attctttttc attgagcatc actcgcaggc
    24061 gcggcattga ttgcaggccg tgctggcgat gcgtctctga acaggcggcc tcacaggtgt
    24121 ggcagccgat acagagcgtg gagtcagcaa ttacaaaacg attcaccagg cattcctcag
    24181 gtgattgtca tttttgacga aaacatgccg atgaaatgtc attttcgaca ctcatcgaca
    24241 cgcccatccc caaacaggcg taacgcctgc aaaacgggca aagcctcagc tcatgctgcc
    24301 gggctttgtc cctttaccag ttggcttaaa ttcaccggca cattgttttc aatggcggtg
    24361 tataagctgt cgtaaacgcc agcgattttt tcgagatagt gttccagaac ctgttcgcga
    24421 tcgcggatgt tgaccttgcc atcaatcatg ccgtcaatgc tcagttgcgc ctgcgcaatc
    24481 cgctgtttgt cttcaccatc ttttgtttcg acgtaatact cgatggtgtg gctgttaaag
    24541 ccatccgcca gggtgacgta aatacgaaaa tgttcaaaaa ggacgaaaca gagttctttg
    24601 tccggcgcgc agctcatggc gtgatgcagg cgcgctttcg ataacgtgat tccctgaacc
    24661 agcgaattgc agtagatgtg ccactggtcc tgtaggcgac gatgccgctg tgcgatgtaa
    24721 tcggctttct cgcttatttc ccaaatagtc attgtcaggt tacccgttta acagagatgc
    24781 cagctttaag cattttttgt gccaactttt aatttattgt tattaaagcg atttttaagc
    24841 taaagatgaa tttcgtcgcc gtgtcgacgt gtcatttcga catcatcgac attattcacc
    24901 gcagggataa tcaacactgg cacaattatt gcttgtagct ggcaatagtt aatgggaggc
    24961 gatatgcacg aaataaccct ctgccaacgg gcactggaat tgatcgaaca gcaggccgca
    25021 aaacacggcg caaaacgcgt aactggggtc tggctcaaaa ttggcgcatt ttcttgtgtc
    25081 gaaaccagct ctcttgcctt ttgttttgat ctggtttgcc gcggcagcgt ggcggaaggt
    25141 tgtaaactgc acctcgaaga acaagaggcc gaatgctggt gtgaaacatg ccaacagtat
    25201 gtgacgctac tgacccagcg cgtccgccgc tgtccacagt gtcatggtga catgctacag
    25261 attgtggcag acgacggttt acagattcgg cggatagaaa tagaccagga gtgagcgatg
    25321 tgtacaacat gcggttgcgg tgaaggcaac ctctatattg agggtgatga acataaccct
    25381 cattccgcgt ttcgtagcgc gccatttgcc ccggcggcac gcccgaagat gaaaatcacc
    25441 ggcattaaag cgcctgaatt tacccccagc cagactgaag aaggcgacct gcattacggt
    25501 catggcgaag cgggcactca cgcgccgggc atgagccagc gtcggatgct ggaagtcgaa
    25561 attgacgtgc tggacaaaaa taaccgtctg gctgaacgca accgcgcgcg ctttgctgcc
    25621 cgcaagcaac tggtgctcaa cctggtttcc agccctggtt ccggtaaaac caccctgctg
    25681 acggaaactt taatgcgcct gaaagacagc gttccgtgcg cggttattga aggtgaccag
    25741 caaaccgtga acgatgccgc acgcattcgc gctaccggca ccccagcgat tcaggtgaac
    25801 accggtaaag gctgccatct tgacgcacag atgattgccg acgccgcgcc gcgcctgcca
    25861 ctggacgata acggtattct gtttatcgaa aacgtcggta acctcgtttg cccggccagc
    25921 ttcgatctcg gtgaaaaaca caaagtggcg gtgctttccg ttaccgaagg tgaagacaaa
    25981 ccgctgaaat atccgcacat gtttgccgcc gcctcgctga tgctgctcaa caaagttgac
    26041 ttgctgccgt atctcaattt cgacgttgag aagtgcatcg cctgcgcccg tgaagtcaat
    26101 ccagaaattg aaatcatcct tatttccgcc accagcggcg aagggatgga ccagtggctg
    26161 aactggctgg agacacagcg atgtgcatag gcgttcccgg ccagatccgc actattgacg
    26221 gcaaccaggc gaaagtcgac gtctgcggca ttcagcgcga tgtcgattta acgttagtcg
    26281 gcagctgcga tgaaaacggt cagccacgcg tgggccagtg ggtactggtt cacgttggct
    26341 ttgccatgag cgtaattaat gaagccgaag cacgcgacac tctcgacgcc ttacaaaaca
    26401 tgtttgacgt tgagccagat gtcggcgcgc tgttgtatgg cgaggaaaaa taatgcgttt
    26461 tgttgatgaa tatcgcgcgc cggaacaggt gatgcagtta attgagcatc tgcgcgaacg
    26521 tgcttcacat ctctcttaca ccgccgaacg ccctctgcgg attatggaag tgtgtggcgg
    26581 tcatacccac gccattttta aattcggcct cgaccagttg ctaccggaaa acgttgagtt
    26641 tatccacggt ccgggttgcc cggtgtgcgt actgccaatg ggtagaatcg acacctgcgt
    26701 ggagattgcc agccatccgg aagtcatctt ctgtaccttt ggcgatgcca tgcgcgtacc
    26761 agggaaacag gggtcgctgt tgcaggcaaa agcacgcggt gccgatgtgc gcatcgttta
    26821 ctcgccaatg gatgcgttaa aactggcgca ggagaatcca acccgtaaag tggtattctt
    26881 cggcttaggt tttgaaacca ccatgccgac caccgccatc actctgcaac aggcgaaagc
    26941 ccgcgatgtg cagaattttt acttcttctg ccagcacatt acgcttatcc cgacgctgcg
    27001 cagtttgctg gaacagccag ataacggtat cgacgcgttc ctcgcgccgg gccacgtcag
    27061 tatggttatc ggcactgatg cctataattt tatcgccagc gattttcatc gcccgctggt
    27121 ggtcgctggt ttcgaacccc ttgatctact gcaaggcgtg gtgatgctgg tggagcagaa
    27181 aatagcggcc cacagcaagg tagagaatca gtatcgtcgg gtggtaccgg atgccggtaa
    27241 cctgctggcg caacaggcga ttgccgatgt gttctgtgtc aacggcgaca gtgaatggcg
    27301 cggcttaggc gtgattgaat cttctggcgt gcacctgacg ccggattatc aacgattcga
    27361 tgccgaaaca catttccgcc cggcaccgca gcaggtctgc gatgacccgc gtgcgcgttg
    27421 tggcgaagtc ttgacgggca aatgtaagcc gcatcaatgc ccgctgtttg gtaacacctg
    27481 taatcctcaa accgcgtttg gtgcgctgat ggtttcctcc gaaggagcgt gcgccgcgtg
    27541 gtatcagtat cgtcagcagg agagtgaagc gtgaataata tccaactcgc ccacggtagc
    27601 ggcggccagg cgatgcagca attaatcaac agcctgttta tggaagcctt tgccaacccg
    27661 tggctggcag agcaggaaga tcaggcccgt cttgatttgg cacagctggt agcggaaggc
    27721 gaccgtctgg cgttctcaac cgacagttac gtcattgacc cgctgttctt tcctggcggt
    27781 aatatcggca aactggcgat ttgcggcacc gccaatgacg ttgcggtcag tggcgcaatt
    27841 ccgcgctatc tctcctgtgg ctttatcctc gaagaaggat tgccgatgga gacactgaaa
    27901 gccgtagtga ccagcatggc agaaaccgcc cgcgcggcag gcattgccat cgttactggc
    27961 gataccaaag tggtacagcg cggcgcggca gataaactgt ttatcaacac cgctggcatg
    28021 ggcgcaattc cggcgaatat tcactggggc gcacagacgc taaccgcagg cgatgtattg
    28081 ctggttagcg gtacactcgg cgaccatggg gcgactatcc ttaacctgcg tgagcaacta
    28141 gggctggatg gagaactggt cagcgactgc gcggtgctga cgccgcttat tcagacgctg
    28201 cgtgacattc ccggcgtgaa agcgctgcgt gatgccaccc gtggtggtgt aaacgcggtg
    28261 gttcatgagt tcgcggcagc ctgcggttgt ggtattgaac tttcagaagc ggcactgccg
    28321 gttaaacctg ctgtgcgcgg cgtttgcgaa ctgctgggac tggacgccct taactttgcc
    28381 aacgaaggca aactggtgat cgccgttgaa cgcaacgcag cagagcaagt gctggcggcg
    28441 ttacattccc atccattggg aaaagatgcg gcgctgattg gcgaagtagt agaacgtaaa
    28501 ggtgttcgtc ttgccggtct gtatggcgtg aaacgaaccc tcgatctacc gcacgccgaa
    28561 ccgcttccgc gtatatgcta ataaaattct aaatctccta tagttagtca atgacctttt
    28621 gcaccgcttt gcggtgcttt cctggaagaa caaaatgtca tatacaccga tgagtgatct
    28681 cggacagcaa gggttgttcg acatcactcg gacactattg cagcagcccg atctggcctc
    28741 gctgtgtgag gctctttcgc aactggtaaa gcgttctgcg ctcgccgaca acgcggctat
    28801 tgtgttgtgg caagcgcaga ctcaacgtgc gtcttattac gcatcgcgtg aaaaagacac
    28861 ccccattaaa tatgaagacg aaactgttct ggcacacggt ccggtacgca gcattttgtc
    28921 gcgccctgat acgctgcatt gcagttacga agaattttgt gaaacctggc cgcagctggc
    28981 cacaggtggg ctatacccca aatttggtca ctattgcctg atgccactgg cggcggaagg
    29041 gcatattttt ggtggctgtg aatttattcg tcatgacgat cgcccctgga gcgaaaaaga
    29101 gttcaatcgt ctgcaaacat ttacgcagat cgtttctgtc gtcaccgaac aaatccagag
    29161 ccgcgtcgtt aacaatgtcg actatgagtt gttatgccgg gaacgcgata acttccgcat
    29221 cctggtcgcc atcactaacg cggtgctttc ccgcctggat atggacgaac tggtcagcga
    29281 agtcgccaaa gaaatccact actacttcga cattgacgat atcagcatcg tcttacgcag
    29341 ccaccgtaaa aacaaactca acatctactc cactcactat cttgataaac agcatcccgc
    29401 tcacgaacag agcgaagtcg atgaagccgg aaccctcacc gaacgcgtct tcaaaagtaa
    29461 agagatgctg ttgattaatc tccacgagcg ggacgattta gccccctatg aacgcatgtt
    29521 gttcaacacc tggggcaacc agattcaaac cttgtgcctg ttaccgctga tgtctggcga
    29581 caccatgctg ggcgtgctaa aactggcgca atgcgaagag aaagtcttta ccactaccaa
    29641 tctgagctta ctgcgccaga ttgccgaacg tgtggcaatc gctgtcgata acgccctcgc
    29701 ctatcaggaa attcatcgtc tgaaagaacg gctggttgat gaaaacctcg ccctgaccga
    29761 gcagctcaac aatgttgata gtgaatttgg cgagattatt ggccgcagcg aagccatgta
    29821 cagcgtactt aaacaagtgg aaatggtggc gcaaagtgac agtaccgttc tgatcctcgg
    29881 tgaaactggt acaggtaaag agctgattgc ccgcgcgatc cataatctca gtgggcgtaa
    29941 taatcgccgt atggtcaaaa tgaactgcgc ggcgatgcct gccggattgc tggaaagcga
    30001 tctatttggt catgagcgtg gggcttttac cggtgccagc gctcagcgca ttggtcgttt
    30061 tgaactggcg gataaaagct cactattcct cgatgaagtg ggcgatatgc cgctggagtt
    30121 acagccgaag ttgctgcgtg tattacagga acaggagttt gaacgtctcg gcagcaacaa
    30181 aatcattcag acggacgtac gtttaattgc cgcgactaac cgcgatctga aaaaaatggt
    30241 cgccgaccga gagttccgta gcgatctcta ttaccgtctg aacgtattcc cgatccacct
    30301 gccgccacta cgcgagcgtc cggaagatat tccgctgctg gcgaaagcct ttaccttcaa
    30361 aattgcccgt cgtctggggc gcaatatcga cagcattcct gccgagacgc tgcgcatcct
    30421 gagcaacatg gaatggccgg gcaacgtgcg tgagctggaa aacgtcattg agcgcgcggt
    30481 attgctaaca cgcggcaacg tgctgcagct gtcattgcca gatattgctt taccagaacc
    30541 tgaaacgccg cctgccgcaa cggttgtcgc ccaggagggc gaagatgaat atcagttgat
    30601 tgttcgcgtg ctgaaagaaa caaacggcgt ggttgccggg cctaaaggcg ctgcgcaacg
    30661 tctggggctg aaacgcacga ctctgctgtc acggatgaag cgactgggaa ttgataaatc
    30721 ggcattgatt taacagatgt ggttatactc gatcaacatt caatgaaagg aggcagtatg
    30781 gcagtttcag caagaaatca attaaccgga acagttagca cagtagcaat gggcgctgtg
    30841 aatgatgaag tagaactgac gctggcaggt ggtgcaaagc tggtggcaat tgtgactcac
    30901 agcagccagc aagcgctggg tctggcgaaa gggaaagaag cgattgccct gattaaagcc
    30961 ccgtgggtca cgctggcaac ggaagactgt ggactgaaat tctctgctcg caaccagttt
    31021 gctggcagcg tgtcgacaat caccgaaggc gcagtgaacg ccactgtcca tatcaaaacg
    31081 gatgcgggtt ttgaaattgt ggcagtggta acaaatgaaa gtcaggatga aatgaaactc
    31141 accactggct cgcgcgttat tgcattgatc aaagcatcag ccatcctgat tgccaccaaa
    31201 gcgtaaacgc cttatttgcc ggagagtatg gctctccggc agatttattt ctttggacga
    31261 tatttttctg gcaactccgg caccggacgc ttgtcatcga tgagatgacg cacggttaag
    31321 atcggatgac gccacagcat tctcggcccg gcccaacgca taatctgttt catctcttca
    31381 cgctttgcag gctggtaaca gtgcaccgga cactgcttac aggctggttt ctcttcaccg
    31441 aacacacatt tatccagccg cttttgcgcg taagcaaaca acgcctcgta atgctccggc
    31501 tctgctgacg cctgcgggca tttcgcttga taaagatcga tcattttttt aatcgtcagt
    31561 ttttcacgag agatacgctt gccggacatg ctgcctccat actcttaagg tgcatttata
    31621 ttacaactta attttaaagg ggactatggc tctgaaggag gaggtttctt caaccgacga
    31681 atttggcgat cttgtttcat cgtcatcacg caaaagctgc aaagcagcat ttttcgcgga
    31741 accgacatca agaactcacc tttcgcgtct tcccctgaaa tgattaactc cggtatcatg
    31801 tgcgccttat gtgattacaa cgaaaataaa aaccatcaca ctacatttaa catcagggag
    31861 ccggacttaa ccccatgaga acaatagaaa atttcgacgc ccatacgcct atgatgcagc
    31921 agtatctcaa gctgaaagcc cagcatcccg agatcctgct gttttaccgg atgggcgact
    31981 tttatgaact gttttatgac gacgcaaaac gggcgtcgca actgctggat atttcactga
    32041 ccaaacgcgg tgcttcagcg ggagagccaa tcccgatggc ggggattccc taccatgcgg
    32101 tggaaaacta cctcgccaaa ctggtgaatc agggcgaatc ggttgctatt tgtgaacaga
    32161 ttggcgatcc ggcgaccagc aaagggccgg tcgagcgcaa agttgtccgt atcgttacgc
    32221 cgggcaccat cagcgatgaa gcgctgttgc aggaacgtca ggacaacctg ctggcggcta
    32281 tctggcagga cagcaagggg ttcggctacg ccacgctgga tatcagctcc gggcgttttc
    32341 gcctgagcga accggcggac cgcgaaacga tggcggcaga actgcaacgt acgaatccag
    32401 cggaattact gtatgcggaa gatttcgccg agatgtcgct gattgaaggt cgccgcggcc
    32461 tgcgtcgtcg cccgctgtgg gagtttgaaa tcgacaccgc tcgccagcaa ttgaacctgc
    32521 aatttggcac ccgagatctg gtcggttttg gtgtggagaa cgcaccacgc ggactttgtg
    32581 ctgccggttg tctgttgcag tatgcgaaag atacccaacg cacgaccctg ccgcatattc
    32641 gttctatcac tatggaacgt cagcaggaca gcatcattat ggatgccgcg acgcgtcgta
    32701 acctggaaat tactcagaac ctggccggcg gtgcggaaaa tacgctggct tcagtgctcg
    32761 actgtactgt aacgccgatg ggtagtcgta tgctgaaacg ctggctgcat atgccagtgc
    32821 gcgatacccg cgtgttgctt gagcgccagc aaactattgg cgcattgcag gatttcaccg
    32881 ccgagctgca gccggtactg cgtcaggtcg gcgacctgga acgtattctg gcgcgtctgg
    32941 ctttacgaac tgctcgtcca cgcgatctgg cccgtatgcg tcacgctttc cagcaactgc
    33001 cggagctgcg cgcgcaactg gaaggtgtcg atagcgcccc ggtacaagca ctgcgtgaga
    33061 agatgggcga gtttgccgaa ctgcgcgacc tgctggagcg agcaatcatc gacacgccgc
    33121 cggtgctggt acgcgacggt ggtgttattg cgactggtta taacgaagaa ctggatgaat
    33181 ggcgcgcgct ggctgacggc gcgaccgatt atctggagcg tctggaagtc cgcgagcgtg
    33241 aacgtactgg cctggacacc ctgaaagtcg gctttaacgc ggttcatggt tattacattc
    33301 agatcagccg tggacaaagc catctggcac ccatcaatta catgcgtcgc cagacgctga
    33361 aaaacgccga gcgctacatt attccagagc taaaagagta cgaagacaaa gttctcactt
    33421 caaaaggcaa agcactggca ctggaaaaac agctttatga agagttgttc gacctgctgt
    33481 tgccgcatct ggaagcgttg caacagagcg cgagcgcgct ggcggaactc gacgtgctgg
    33541 tgaacctggc ggaacgggcc tatacgctga actacacctg cccgaccttt attgataaac
    33601 cgggtattcg catcaccgaa ggccgccatc cggtggttga acaggtgctg aatgagccgt
    33661 ttatcgccaa cccgctgaac ctgtcgccgc agcgtcgcat gttgatcatt accggtccga
    33721 atatgggcgg taaaagtacc tatatgcgcc agaccgcact gattgcgctg atggcctaca
    33781 tcggcagcta tgtaccggca caaaaagtcg agattggacc tatcgatcgc atctttaccc
    33841 gcgtaggggc agcggatgat ctggcatccg ggcgttcaac ctttatggtg gaaatgaccg
    33901 aaaccgccaa tattttacat aacgccaccg agtacagtct ggtgctgatg gatgagattg
    33961 ggcgtggaac gtccacttac gatggtctgt cgctggcgtg ggcgtgcgcg gaaaatctgg
    34021 cgaataagat taaagcgttg acgttgtttg ctacccacta tttcgagctg acccagttac
    34081 cggagaaaat ggaaggcgtc gccaacgtgc atctcgatgc actggagcac ggcgacacca
    34141 ttgcctttat gcacagcgtg caggatggcg cagcgagcaa aagctacggc ctggcggttg
    34201 cagctctggc cggcgtgcca aaagaggtta ttaagcgcgc acggcaaaaa ctgcgtgagc
    34261 tggaaagcat ttcgccgaac gccgccgcta cgcaagtgga tggtacgcaa atgtctttgc
    34321 tgtcagtgcc agaagaaacg tcgcctgcgg tcgaagcact ggaaaatctc gacccggatt
    34381 cactgactcc gcgtcaggcg ctggagtgga tttatcgctt gaagagtctg gtgtaataac
    34441 aattcccgat agtcttttgc tatcgggaat attaacgaca actgacgaat caaataaaaa
    34501 taccctgtat aataggaaat cttattttac agggtaaaac catgccatct acacgctacc
    34561 aaaaaatcaa tgcccatcac tatcaccata tatgggccgt tggtgatatt catggtgact
    34621 atcaattatt acaatcccgc ttacatcaac tctctttttg tcctgaaacc gacttgctta
    34681 tttctgtcgg cgataacatc gatcgtgggc ccgagagtct taacgtgctg cgcctgttaa
    34741 accagccctg gtttatctcc gtcaaaggca accacgaagc gatggcgctt gaggcattcg
    34801 aaactggcga tggaaatatg tggcttgcca gcggtggtga ctggtttttc gatttaaatg
    34861 attcagagca acaagaggca atagatctgt tgctgaaatt ccatcacctt ccacatatta
    34921 ttgaaatcac taacgacacc ataaaatatg tcatcgcaca tgcagattat ccggggaagg
    34981 agtatcagtt tggcaaagaa atagcggaga gcgaattact ctggcctgtt gatcgtgtgc
    35041 agaaatcact taatagcgaa ttacaaaaaa taaacggcgc tgattatttt atatttggac
    35101 atatgatgtt tgataacatt cagacgttcg caaaccagat ttatattgat accggatcgc
    35161 cgaaaagcgg gcggctgtca ttttataaaa taaagtagtc tcatgcttct tctgtgaagc
    35221 atgagtaacc cggcgttatt gcaggtcatt attcattttt cgctaccagc aaagagagat
    35281 ccagcttcgc cagcgcgcgg ctggcactct ccggcagacc atcgtcagta atgatctgat
    35341 caaactcgct taatggtaac gccagccatg tcgccacctg tccatatttc gtcgcatcac
    35401 agaccaaaac tcgctggcgg ctggcactgg caatcgcccg tttcaccgtg actttatctt
    35461 ctgctggcgt agagatcccc cgcacactcc atgacgatgc agaaataaaa gcctgatcaa
    35521 tcatcaggct acgcagcatg gtcgcagcgg cttccccaac acaggaacgg ttttcccgac
    35581 acactgcgcc gccagtgtga ataattgtgc aattactgtt gtcgagcaag tagtccgcaa
    35641 taacgaagtc gttagtaacc actgtgagtg gctccatgtg agtcagatgc tgtgcaatcg
    35701 ctaacgtggt cgttcccgca tccagataga tacaacgtcc cggctgaaca agacttgccg
    35761 ccagcttgcc aatagccgct ttttgcgtca ttgccagcgc agtttttacc tgatgagaag
    35821 gttcatgcgc cacccgtccc ggagactgga cgcccccgga caccaacaca acggctccct
    35881 gctgctccag tttttgtaaa tcccgacgaa tggtcatatg tgacacattc attctgtccg
    35941 ttagttcagc aatactaaca atgccttttt cagctaccat ctcaaggatg atttggcgac
    36001 gctctacggg tatcaacttt tgctccttcc tttgtcctgc tgacattcta cgctatttgc
    36061 ctgcgaaacg tgcgcggcgc aactaacgct tagttcacat aaaataacat acaatgttaa
    36121 tttttgtgaa tcagctcacc atatcgttat ctttccgcgc ttatattcac aacatcaaac
    36181 aaaatatcac ttaaattaac aaggagagca gatgaaaacg ggatcggagt ttcatgtcgg
    36241 tatcgttggc ttagggtcaa tgggaatggg agcagcactg tcatgtgtcc gcgcaggtct
    36301 ttctacctgg ggcgcagacc tgaacagcaa tgcctgcgct acgttgaaag aggcaggtgc
    36361 ttgcggagtt tctgataacg ccgcgacgtt tgccgaaaaa ctggacgctc tgctggtgct
    36421 ggtggtcaat gcggcccagg ttaaacaggt gctgtttggt gaaacaggcg ttgcacaaca
    36481 tctgaaaccc ggtacggcag taatggtttc ttccactatc gccagtgctg atgcgcaaga
    36541 aattgctacc gctctggctg gattcgatct ggaaatgctg gatgcgccag tttctggtgg
    36601 tgcagtaaaa gccgctaacg gtgaaatgac tgtcatggcc tccggtagcg atattgcctt
    36661 tgaacgacta gcacccgtgc tggaagccgt tgccggaaaa gtttatcgca taggtgcaga
    36721 accgggacta ggttcgaccg taaaaattat tcaccagttg ttagcgggcg tacatattgc
    36781 tgccggagcc gaagcgatgg cacttgcagc ccgtgcgggg atcccgctgg atgtgatgta
    36841 tgacgtcgtg accaatgccg ccggaaattc atggatgttc gaaaaccgga tgcgtcatgt
    36901 ggtggatggc gattacaccc cgcattcagc cgtcgatatt tttgttaagg atcttggtct
    36961 ggttgccgat accgccaaag ccctgcactt cccgctgcca ttggcctcaa cagcattgaa
    37021 tatgttcacc agcgccagta acgcgggtta cgggaaagaa gacgatagcg cagttatcaa
    37081 gattttctct ggcatcaccc taccgggagc gaaatcatga tcaagattgg cgttatcgcc
    37141 gatgatttta ccggcgcgac ggatatcgcc agttttctgg tggaaaacgg tctaccaacg
    37201 gtacaaatta acggtgttcc aacaggtaaa atgccggaag caatcgacgc actggtgatc
    37261 agcctgaaaa cgcgctcctg tccggtggtt gaagccacac agcaatcgct ggcggctctg
    37321 agctggttgc aacagcaagg ttgcaaacag atctatttca aatactgctc tactttcgac
    37381 agttcggcga aaggtaatat cggcccggtt accgatgcat taatggatgc tctcgacacg
    37441 ccgtttacgg tcttctctcc ggccctgccg gtcaacggac gtacggttta tcaggggtat
    37501 ttgttcgtaa tgaatcaact gctggccgaa tccgggatgc gccatcaccc ggtaaatccc
    37561 atgaccgaca gctatcttcc ccgtctggtt gaagcgcaat ccacagggcg ctgcggcgtc
    37621 gtttcggcac atgttttcga acaaggtgtg gatgccgttc gtcaggagct ggctcgctta
    37681 cagcaagagg gctaccgcta cgcggtgctt gatgcgctga ccgaacacca tctggaaatt
    37741 cagggagaag ccttgcgcga taccccactg gtaacgggcg gttctggtct ggcgattggc
    37801 cttgcccggc agtgggcgca agaaaacggt aaccaggctc gcgaagcagg gcgtccgctc
    37861 gctgggcgcg gcgtagtgct ctccggttca tgctctcaaa tgaccaaccg ccaggtggca
    37921 cattaccgtc aaattgcacc agcccgtgag gttgatgtgg cacgctgcct ctcaaccgaa
    37981 actctggccg cttatgcaca cgagctggca gagtgggttc tgggccagga aagtgtactt
    38041 gctccactgg tttttgccac cgccagcact gacgcattgg cagcaattca acagcaatac
    38101 ggtgcacaaa aagccagtca ggcagtggaa acactgtttt ctcaactagc ggcgcggtta
    38161 gcagcggaag gcgtgacacg ctttattgtc gcaggcggtg agacctccgg cgtagtcaca
    38221 cagagcctgg gcataaaagg ttttcatatt ggcccaacca tttcccccgg cgtgccgtgg
    38281 gtaaacgcac tggataagcc tgtctcactc gcccttaaat ctggcaactt cggtgatgaa
    38341 gccttttttt cacgagccca aagagagttt ttatcatgag cgatttcgca aaagtagagc
    38401 agtctttgcg agaggagatg acgcggattg ccagttcatt ctttcagcgc ggatatgcaa
    38461 ctggttcggc tggcaatctg tcgctgcttt tacctgacgg gaatttactg gcgacaccga
    38521 caggttcatg cctgggcaat ctcgatccgc agcggctttc caaagtcacc gcggatggcg
    38581 aatggttaag tggtgacaaa ccctcgaaag aggtgctctt tcatctggcg ctgtatcgca
    38641 acaatccgcg ctgtaaagcg gtagtgcatt tgcacagtac gtggtcgacg gcgctttcct
    38701 gcctggaagg gctggacagc aataacgtta ttcggccgtt cacaccatac gtggtgatgc
    38761 ggatgggaaa cgtcccgctg gtgccctatt accgaccggg cgataaacgc atcgcacagg
    38821 atctggcgga actggcagca gacaatcagg cttttttact ggcaaatcat ggcccagtgg
    38881 tttgcggtga aagcctgcaa gaagccgcca acaatatgga agagctagag gaaacggcaa
    38941 agctgatttt tattctcggt gaccgcccga tccgttatct gaccgcaggt gaaattgcgg
    39001 aattaaggag ttaatgcaat gcctcgtttt gcagctaatt tatccatgat gttcaccgaa
    39061 gtgcctttta ttgaacgctt cgccgcagcg cgaaaagccg ggttcgatgc tgtggagttt
    39121 ctgtttccct atgactactc cccccagcaa atccagcagc aactggcgca aaaccacctg
    39181 acgctggcgc tgttcaatac cgctcccgga gatattaatg cgggagaatg gggattatcc
    39241 gcccttcccg gacgtgagca cgaagcacgt gcagacatcg acctggcact ggaatatgca
    39301 ttggcgctca attgtgaaca agtccatgtg atggcaggcg tcgtgcccgc tggcgaagat
    39361 gctgaacggt accgggcagt atttattgat aatctccgct acgctgccga ccactttgcg
    39421 ccacacggca agcgaatatt ggttgaagca ctcagccccg gcgttaagcc tcattatctc
    39481 ttctccagcc agtatcaggc actggctatt gttgaagagg ttgcgcgaga taacgttttt
    39541 attcaactag atactttcca tgcgcaaaaa gtagatggca acctgacgca tttaatccgc
    39601 gactacgccg ggaaatatgc gcacgtacaa attgccggac tacctgatcg acatgaaccg
    39661 gacgatggag aaatcaacta tccgtggctg ttccgcctgt tcgatgaagt gggatatcag
    39721 ggctggatcg gttgtgaata caaacctcgt ggcctcaccg aagaaggact tggctggttt
    39781 gacgcctggc gctaattcgt taaatcccta attacaacgt acccatacat ccccccatct
    39841 acggatgggg taggttaact ataactttca gacagggttt cccatgtcca caattacatt
    39901 gttatgcatt gcgttaactg gcgtaatcat gctgttgctg ctggtcatca aggcaaaggt
    39961 acaaccattc gttgctctgc tcctcgtcag cctgttagtc gcacttgcgg cgggtatacc
    40021 ggcgggcgaa gtgggtaaag tgatgatcgc cgggatgggc ggtgtgcttg gttccgtcac
    40081 tattattatt ggtctgggcg ctatgctggg gcgtatgatc gaacactctg gtggtgcaga
    40141 gtcactggct aattatttca gtcgcaagtt aggcgacaaa cgaactatcg ctgcgctgac
    40201 tctggcagcg ttcttcctcg gtattcccgt cttctttgat gtcggcttta ttattcttgc
    40261 gccaatcatt tacggttttg ccaaggttgc caaaatatca ccactcaaat ttggtctgcc
    40321 tgtcgctggg atcatgctca ctgttcacgt ggcggtaccg cctcatccag gccctgtcgc
    40381 cgcagcgggg ttactccacg cagacatcgg ctggctaacc atcatcggta ttgcgatttc
    40441 tattcccgta gggattgttg gctactttgc agcgaaaata atcaataagc gccaatatgc
    40501 gatgtcggtc gaggtactgg aacagatgca actggctccg gccagtgagg aaggcgcgac
    40561 aaaattaagc gataaaataa atccaccggg cgtcgcgctg gtcacctcgc taattgttat
    40621 tcctatcgcg attatcatgg cgggtacggt ttccgcaaca ctgatgccgc cttcgcatcc
    40681 cctgcttggt acgctacagc tgatcggctc accaatggta gctctaatga ttgcgctggt
    40741 gctggcattc tggttattgg ctttacgtcg cggctggagc ttacaacaca ccagcgacat
    40801 tatgggctca gcccttccca ctgcggcggt agtgattttg gttactggtg ctggaggggt
    40861 atttggcaaa gtgctggtgg aatcgggcgt cggtaaagcc cttgccaaca tgttacaaat
    40921 gattgacctg cctctgttac cagccgcatt tattatttca ctggcgctgc gtgcatcgca
    40981 ggggtcagca accgtagcaa tcctgacaac cggcgggtta ctctcagaag cggtgatggg
    41041 attgaatcca attcagtgcg tattggtgac gctggcagcc tgctttggtg ggcttggcgc
    41101 gtcacatatt aatgactcag ggttctggat tgtgaccaaa tatctggggt tgtcggtagc
    41161 agacgggctg aaaacctgga cagtgttaac gaccattctc ggttttaccg gattcttaat
    41221 tacctggtgc atatgggcgg taatttgacc ttaataaaag gtccgatggg catcggacct
    41281 tttatttagc gcttaccttc cgccagcagc ggcgggatgc tcggcaccat cggcgcgtca
    41341 tcaatatctt tctgcgtcat gcggaacgct tcgggataat gttcgcggct ggtgcggcgt
    41401 agcggttcgg tatcgcgcca ggtatagagg caatgctggc actggtagac catccagaca
    41461 tcttttaccg gcgatttcgc catcacttca atctgttcat cggcacaacg tggacaaatc
    41521 atcttgttct ccttattgac gtgcggccag catagcggtc agtttttcag cccaggcttt
    41581 ggtttccggt aagtccacca ccggctggct gtagtgacca cggttgtccg gggcgacagg
    41641 cgtggtggcg tcgataatca gcttgtcggt gatccccgcc ggacttgagc cagggtcgag
    41701 ttccagcacg gacatattcg gcaactgtac caaatcccct gccgggttca ctttcgagga
    41761 gagcgcccac atcacctgcg gcaggttgaa cggatcaacg tcttcatcga ccataatcac
    41821 catcttcacg tagcccagac cgtgcggcgt ggtcatcgca cgcaggccca ccgcgcgggc
    41881 aaagccgccg tagcgttttt tggtggagat aatcgccagc aggccgtggg tgtacatggc
    41941 gtttaccgcc tgcacttccg ggaactcggc tttcagttgt tgatacagcg gcacgcaggt
    42001 ggcaggcccc atcaggtagt cgatttcggt ccacggcata ccaagataga gcgattcgaa
    42061 aatcggtttg gtacggtaag agactttatc gatacgcacc acggtcatgt tacgcccgcc
    42121 ggagtagtgg ccggtaaact caccgaacgg cccttcaatc tcacgtttac gactttcgat
    42181 aaccccttcg aggatcactt ccgagcccca cggcacatca aaaccggtca gcggggcggt
    42241 ggcgatcggg tacgggcttt cgcgcagcgc gcctgccatt tcgtactcag actgatcgta
    42301 tttcagcggc gtggccccca ttagggtgat gatcggatcg ttaccgagtg tgatggcaat
    42361 cggcagatct tcaccgcgct cttctgcttt atgcagatgc agggcgatat cgtgcatcgg
    42421 caccggttgc aggccgagct tacgcttgcc cttcacttcc atgcggtaga tgccgacgtt
    42481 ctgcttgccg aagttatccg ggtcgagcgg atcgcgggaa accacgcacg ctttatcgag
    42541 atagaaaccg ccatcaccgt cgtttaaacg aaacagcggc agaatattga acaggttgat
    42601 ctcgtcgcca tcgacggtgt tctgcgccca ggctggattg gcgcggcgct ccggggcaat
    42661 ggggaagtta tcccagcggc ggataaactc atcaatctgt tttttaaccg gggtgtttgg
    42721 cggcaggccg agggaaatcg cgtggttctg ccaggaaccg atggtgttca ttgccacgcg
    42781 ggcatcgata aagccgcgaa tattatcaaa ccaaagcgcg ggcgcgccgt cgccgatacg
    42841 cccggtggcg ttggctgcgg ctgccagatc cggttccgcg ttcacctctt cgctgatttt
    42901 cagtaactgg ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat caaatgccat
    42961 tattcattct cctgagaaaa attccgggcc tgcggcaatc cttgccagcg cctggcgtga
    43021 ggatgttcga ggccaaattg atccagcacg cgggctacca cgtggtggac aatgtcatct
    43081 accgtttcgg gatggttata aaaggcaggc atcggcggca ccatcgccac gcccatgcgc
    43141 gaaagtgcga gcatattttc gagatggatt gtgctaagcg gcatttcacg cggcaccagc
    43201 accagtttgc ggccttcttt gagcacgacg tccgccgcgc gccccaccag gccatcggcg
    43261 taaccagcgc ggataccggc gagcgttttc atactgcatg gaataacgat catgccgtcg
    43321 gtacgaaagg agccggaaga gatagtcgcc gcctgatccg ccgggttatg gctgaagtct
    43381 gcgagggcgg caacatcgcg agcgctgtaa ggcgtttcca gttcaatcgt ggttttcgcc
    43441 cacttcgaca tcactagatg agtctcgaca ttcggcatct cccgcagcgc ttgcagtaat
    43501 gccacaccaa gaggcgcacc ggtagcccct gtcatcccga cgatcagttt cattgcttac
    43561 tctcttaatt gttcgtatac gaacgttatt attaacatac tccttcattt ccacagcggc
    43621 aaggattgaa aagtaacaac atcttcttcg tcactgaaaa accgctatga tagcgacaga
    43681 gaattagccc ggagtttaca tggcgttacg aaataaagcg ttccatcagt tacgacagct
    43741 ttttcagcag catacagctc gctggcagca cgagttacct gacctgacca aaccacagta
    43801 cgcggtgatg cgcgccattg ctgataagcc tggtatcgaa caggtggcac tgatagaagc
    43861 agcagtcagc accaaagcaa cgctggcaga aatgttggca agaatggaaa atcgtggctt
    43921 agtcagacgt gaacatgacg ccgctgacaa gcggcgacgc tttgtctggc tgactgccga
    43981 aggagaaaag gtacttgctg cggcgatccc cattggtgac agtgtggatg aggaattttt
    44041 ggggcgtttg agtgcagaag agcaggagct gtttatgcag ttggtgcgca agatgatgag
    44101 caaataggat gctgattgcc gggtaggacg ctgacgagtc ttatccaggc gacaaaacac
    44161 accgacttcc caaacaaaag aaaaaggcca gcctcgcttg agactggcct ttctgacaga
    44221 tgcttactta ttcgcggaac agcgcttcga tattcagccc ctgcgtttgc aggatttcgc
    44281 gcaaacggcg caggccttca acctgaatct ggcgaacacg ttcacgggtg aggccaattt
    44341 cacgacctac atcttccagt gttgccgctt cgtaccccag caaaccgaat cgacgtgcca
    44401 gtacttcacg ctgtttggcg ttcagctcga acagccattt gacgatgctc tgcttcatat
    44461 cgtcatcttg cgtggtatct tccggaccgt tctctttttc atcggccagg atgtccagca
    44521 acgctttttc ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc tcgttaagac
    44581 gaagcatacg gctgacgtca tcaactggct tatccagttg ctctgcgatc tcttccgcac
    44641 ttggttcgtg gtccagttta tgggacaact cgcgtgcggt tcgcaggtaa acgttcagct
    44701 cctttacgat gtgaataggc aaacgaatag tacgggtttg gttcataatc gcccgttcga
    44761 tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt tccgggtcaa
    44821 acttctctac cgcgcggatc agccccaggt tgccctcttc gatcaggtcc agcaacgcca
    44881 gaccacgatt gccataacgg cgggcaattt ttaccaccag acgcaagtta ctctcgatca
    44941 tccggcggcg ggaggcaaca tctccacgca gtgcgcgacg cgcaaaataa acttcttctt
    45001 cggccgttaa cagtggtgaa taaccaatct caccaaggta aagctgagtc gcgtccagca
    45061 cacgctgtgt ggctccctgc gataacagtt cctcttcggc caaatcgtta tcactgggtt
    45121 cctcttctac taaggccttt tcgtcaaaaa cctcaactcc gttctcatca aattccgcat
    45181 cttcatttaa atcatgaact ttcagcgtat tctgactcat aaggtggctc ctacccgtga
    45241 tcccttgacg gaacattcaa gcaaaagcct ggttccgccg atttatcgct gcggcaaata
    45301 acgcagcggg tttacggatt tccccttgta acgaatttca aaatgcaagc gtgttgaact
    45361 ggttccggtg ctacccatgg ttgctatttt ttgccccgcc ttcacttctt gttgttcccg
    45421 gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa tcatcattat gtttgatgat
    45481 aatcagatta ccgtagccgc gcagcgcgtt accggcataa acaacgcggc catctgcggt
    45541 cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc cccttgttgc ccccctcaga
    45601 agcgccaaag gtttcgatca ctttgccctc agtcggccag cgccaggtgg agataggcgt
    45661 actggttgat gtactgctga cagtcggctc ggttgtgctt gctgttggta ccgttacagg
    45721 cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac attttgttag cactctgttc
    45781 acccgaagac tcagaatacg taattgtcgg ttgcgacgca acagcaacgg tggaattttg
    45841 tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg catttccgcc
    45901 agtgattggc gtaccggaag cattacccac ctgtaaggtc tgaccgacgt tcagcgcgta
    45961 tggtgcctga atattgttgc gctgagcaag gtcacggaaa tcgttgccag taatccaggc
    46021 gatatagaaa agtgtgtcgc cttttttcac ggtataggta ctgccgctat aactgccttt
    46081 cggaatgttc ccatactgac ggttgtagac gatgcgtccg ttttccatct gtaccggctg
    46141 ctgagctact ggctgtactg gctggatttg cggttgttga gtagcctgaa tttgtggctg
    46201 ctgtaccggc tgaatttgcg gttgctgcgc tgtagacgtc gtccccattt tcggcggcgg
    46261 cgtaatcaac ataccagaat tggtatttgc aggcgcattg ccattaacgg agctgaccgg
    46321 tgccggtgga tttgaagtgt cagaacagcc tgccagccat agcgaaacca gtgacaaagc
    46381 cgcaatgcgg cgaacggtga attttgggct tcccgcgctc atttatcccc caggaaaaat
    46441 tggttaataa ccagtgacat aattaccgtg caaggcacca tactgaacac tggaaaagat
    46501 gttcacgata cgctgacctg cggcaaaata accaggaaaa atccaggtat ttcctcacgt
    46561 tttaagccag ctcacccttc actaaaggga caaagcgcac ggcctccacg gtatcgataa
    46621 taaattcgcc tccccgacga cgcacccgtt tcaaatactg gtgctcctcc cctacgggta
    46681 agacgagaat tccgccttcg tccagctgcg tcattagcgc agttggaatt tccggcggtg
    46741 ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg ccaaccttgc catccatcgc
    46801 catgacgggt tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc gcctgccact
    46861 gcaagccttt aatccgttca accgagcaaa catgctggac aagatgcgcc aggattgccg
    46921 tttgatatcc cgaaccggtg ccaatttcca gcacccgcga ctgcggcgtc agttcgagta
    46981 attcggtcat tcgcgccacc atatacggct gcgaaattgt ctgcccctga cctatcggca
    47041 aggcgatatt gtcccaggct ttttgttcaa acgcttcatc aacgaatttt tcacgcggca
    47101 cggcggcaag tgcattcagc accagctcat cctgaatacc ttgcgcacgt aattgatcca
    47161 gaagtgcttg tacgcgtctg cttaccattg cgtgccaact cccacgctgt ttaaccagtc
    47221 tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg tgatggaaac
    47281 atagccctca tctaccgcag caaaatcggt ccccggaccg gcatcacatt taccgcccgg
    47341 cgggccaatc cagtacagcg tattgccgcg cggatcttgc tgcgggatca cctgatctgc
    47401 cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg atttgatcca atggtaaatc
    47461 cggaacgtta atattaagaa tacgtccggt gcgcagcggc tctttacaca gtgcgcgcaa
    47521 aattgaacag gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa gcgagacggc
    47581 cagcgccgga aaacctaaat gacggccttc catcgcggcg gctaccgtac cggaataaat
    47641 aacatcatcc cccagattcg gcccggcgtt aattccggac acaacaatgt ccgggcgcgg
    47701 acgcatcaga gcattcacgc caagatagac gcaatcggtc ggggttccca tttgcacagc
    47761 aatatcacca ttttcaaagg taaacgtgcg cagggaggat tccagtgtca gagaatttga
    47821 agcgccgctg cggttacgat cgggggcgac cacctgaacg tcagcaaact cacgcaaggc
    47881 tttcgccagc gtttgtatac cgggtgcatg taccccgtca tcattactca gcaatatgcg
    47941 cataatcacc tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa cttcccgccg
    48001 gaagccagaa acggatttct acggtgacgt catcccacca gttccagctt aatttttgcg
    48061 gatacagcag catcgctctg cgcgcggctt caactttttc acgcaccagt aaagtttgta
    48121 attcagtttc ttcggcgaca gctgcttgtt cgaatgccag cgcttcacgc tgagttcccc
    48181 attcgccact gcccggcaat gcggcggtta tcatcagctc tttatcgttg acgcgacgct
    48241 gtaattccgc cagttcttcg gtggttgcaa caaaccagct accacgtccg gctaattgta
    48301 gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt gaggcgctca gcaacaatct
    48361 gattaaacaa cgcactgcgg gctgccgaca accaaaaact ccgtttattg cgatcgcgca
    48421 ccggagtatt ggtttgcgcc caacgcagcg cgccctgcaa gttgctaccg ccaatcccaa
    48481 aacgttgggc accgaagtag ttcggtacac cttttacgca aatatcgatc agacgttgtt
    48541 caacgtcatc gcgattgctc acttcgcgta gtaccagggt aaaggcgtta cctttcagcg
    48601 cccctaaacg cagcttgcgc ttgtgccgcg catactccag cacctggcag ccttccagtt
    48661 gaaaggcgct cagatcgggc atctctttgc ccggcacgcg agcgcataac cactgttccg
    48721 taacagcatg tttatctttt tgcccagcga agctgacttc acgggcatga attttcagga
    48781 atttcgccag tgcatccgcc acaaaacggg tattgcagcc gtttttgagg attctaacca
    48841 gaatatgctc accttcacca tcaggctcaa agcccaaatc ttccaccacc acaaagtctt
    48901 ccggattggc tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa gtgagattat
    48961 caaactcaat cattttgttg ccttaatgag tagcgccacc gcttcacagg caatcccttc
    49021 cccacgtccg gtaaatccca gtttttccgt agtagtggct ttcacgttaa catcatccat
    49081 atggcagccg agatcttcgg caataaacac gcgcatttgt ggaatgtgcg gcaacatctt
    49141 cggtgcctga gcgatgatag tgacatcgac gttgccgagg gcataacctt tcgcctgaat
    49201 acgacgccag gcttcgcgca acagctcgcg gctgtcggca cctttaaatg ccggatcggt
    49261 atccgggaac agcttgccta tatctcccag cgccgccgcg ccaagcaatg catcggtcaa
    49321 cgcatggagc gccacgtcgc catcagaatg cgccagcagc cctttttcgt aaggaatgcg
    49381 tacgccacca atgataattg ggccttcacc gccaaaggcg tgtacgtcaa aaccgtgtcc
    49441 aattcgcatt atgtattctc ctgatggatg gttcgggtga ggtaaaattc ggccagcgcc
    49501 aaatcctccg ggcgcgttac cttgatgtta tccgcgcggc cttcaacaag ttgcggatga
    49561 aaaccgcaat attccagcgc cgaggcctca tcggtaatgg tagcgccttc atttagagcg
    49621 cgcgtcaggc agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag cgcgtgccat
    49681 aagccgttgc gatcaacggt atgagcaatg gcatttttgc ccggttcggc acgtttcatc
    49741 gtatcgcgca ccggcgcagc gaggatcccc cccgtgcggc tggtttcgct caacgccaac
    49801 aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg cgtcatgcac caatacccac
    49861 tgcgcgtcgc cagcggcttt cagacctgcc agcacggaat cggcacgctc ttcaccacca
    49921 tcaacaaccg tgattcgtgg atgattcgcc agaggaagtt gtgcaaagcg gctatcgcca
    49981 ggacttatgg caatgacgac acgtttcacc ctgggatgcg ccagcagcgc atacaccgag
    50041 tgttcaagaa tggtttgatt accgattgag agatattgct taggacattc cgtttgcatt
    50101 cggcggccaa atccggccgc cggaaccacg gcgcaaacat ccaaatgagt ggttgccatg
    50161 ttaattcccg ggctgattta tcgattattt tgccccgcag actgtgcgcg ctttgacgcg
    50221 tcaggcacca gacgataaaa cgtttcgccc ggcctggtca tgctgagttc attacgcgca
    50281 cgctcttcga gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa aagctgatcg
    50341 tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg ccacatcatt attgacgcga
    50401 gtatagtcat gtataccgtt cttaccgaac cacagcgaat actgtagcca gaccagaata
    50461 gccagcaaca gcagcgttag tttacccatc ctgccccctg aaaaacggca tcttcatccc
    50521 atgcatccga agacgactct acatcctctg ttggggatac cgcgacaacg cgggcaaatg
    50581 taccacattt gtccattgtt acgtataccc agggcgtgca gaacataatc tcattattag
    50641 ttacggattg aattatgaac agaatagaga ggaaagaaca attaacccag tagccacata
    50701 aacagcgcac caaacataat gccaaccgtc gccagagtga aaacaatact gtagcgtagc
    50761 tttccgtcca tcaatgaatg cagtgcaatc cccaccacta cagcgacggg catcagcgcc
    50821 agaaagaaag gccaggtgta gataaaaaag agcagcgtgt tagagccata aatcaacatc
    50881 ggaattgcca acgcgcacag ccacgataaa aaaccaacca cagcgcctgg cagcgaccat
    50941 gtggtgtctt caacctcagt aaggctgtcg ttatttgtta atgtaatgtt gtggctgtta
    51001 cgcatatctg atcctgttac tttgacgaac cgggcatgga aacccggcgg tgtctcagga
    51061 tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat ttgttactaa
    51121 ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg aatctattcc
    51181 cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aaaccttttg gatcgcgggc
    51241 ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt ctcctacgcg
    51301 ttcgcgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca gcaccaccag
    51361 tccggcttca accatcaaat tcgccacttc accaacccga cggatattct ctttacggtc
    51421 ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acgttgtcgc catccagcag
    51481 atacgtactg acgccaagtt tatgtaacgc ctcctccagc gcccccgcga cggttgattt
    51541 accggaacca gagaggccgg taaaccacag cacaacacca cgatgaccgt ggtgctgttc
    51601 gcgttgttgc ggagttactg ggtggctatg ccagacgacg ttttcgtcat gcagcgccat
    51661 tatttatctc ccagtaaatc gcgcgcaccc caatgcggga aatggcggcg aaccagggca
    51721 ttcaattcca gttcgaatgc actgaattca gatggcgcag cagttgcctg gctaactggt
    51781 tcgtgcacca taccggcacc tacggtcaca ttactcaggc gatcgataaa aatcagccca
    51841 ccggtaaccg ggttttgctg ataacgatct aacaccagtg gctcgtcaaa ggtgagatct
    51901 accaggccga tgccgttcag cggcaggttt tcaacttcgc gttgggtaag gttattgata
    51961 tcaacctgat aacgaatgcc atcaacacga gcacgcgtct tcttaccggc aattttgatg
    52021 tcataactct ggcccgggga aagcggctgt tccgccatcc ataccacatc caccgacgcg
    52081 ctctgcacag ccggtaacgc ttcgtctgcc gccagcagca gatcgccacg gctgatgtcg
    52141 atctcatccg tcagcaccag ggtgatcgct tctccggcaa aggcttcttc gcgatcgcca
    52201 tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcac tttgacacgt
    52261 tgcccgactt ccacgcgacc ggatgccagc gttccggcgt agccacgaaa atcgagattc
    52321 ggacggttaa cgtactgcac cgggaagcgc attggctgag catccaccac tcgctggatc
    52381 tccacggttt ccagcacttc tagcagtgtc ggaccgctgt accacggcat actttcactt
    52441 tgcgaagcca cgttgtcgcc ttccagtgcg gagagcggca caaagcggat atccagatta
    52501 cccggcagct gcccggcaaa agtcagataa tcttcacgaa tacgggtgaa cgtctcttca
    52561 ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat ccccaacagt
    52621 gtggagataa aactatgacg acgggtttga tcgagcacgc ctttacgggc atcgatcagt
    52681 aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgggtgta ctgctcgtgc
    52741 cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaatagcg gtaggccaca
    52801 tcaatagtga tgccctgttc gcgctcagct tgcaggccgt ccaccagcag tgccagatcc
    52861 agcttttcgc cctgggtgcc gtgacgctta ctgtcgttat gcagcgatga gagctgatct
    52921 tcgtagattt ggcgggtatc gtgcagcagg cgaccaatca gggtactttt gccgtcatcg
    52981 acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat catccatgct
    53041 tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt aaaaataccc
    53101 ctgacgtttt ttcagctcca tagaccccgc ctggtcgcgg tcaattacgc ggccctgacg
    53161 ttcactggtg gtggaaacca gcatctcttc gatgatctcc ggcagtgttt gcgcatttga
    53221 ctccaccgcg ccggtcagcg gccaacagcc cagtgtgcgg aaacgcacca tccgtttttt
    53281 aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca acataccgtc
    53341 gcgttccaga accggacgtt ccgcagcaag atacagcgga acaatgtcga tattttccag
    53401 ccagatgtat tgccagatat cctgctcggt ccagttagag agcgggaaga cgcggatgct
    53461 ttcgcctttg ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct gatttttcgg
    53521 atcccagcga tggaagcggt cacggaaaga gtagattcgc tctttagcgc gggatttctc
    53581 ttcgtcacgg cgcgcaccac cgaaggcggc atcaaaaccg tatttgttca gcgcctgttt
    53641 caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgtacga atggattaat
    53701 ccccatcgcc acgccttccg ggtttttatg caccagcagc tcgcagccgt aggctttcgc
    53761 cgtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcga catgcagcaa
    53821 cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca tgacgctgga
    53881 atctttaccg atagagtaga gcatcaccgg atttgagaat tctgcagcca cctcgcgaat
    53941 aatgtggatg ctttccgcct ccagttgccg caggtgggta agtcgttttt gatccataac
    54001 cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata gggagcgtaa
    54061 gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa cgatttggca
    54121 aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa gatttgaaat
    54181 tgttttagcg cagcggcagt ttcatactat gacggtaaaa aaatttgcat ggtatttaag
    54241 gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc ggcgtatgct
    54301 ttatcctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat acccaggcac
    54361 gacatattgc tactttcttt ccgggacgca tgaccggaac tcctgcagaa atgttatctg
    54421 ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt cggacattta
    54481 atagtcggta tatttatacc gcccgcgata atcgcaagag ctggcataac gtgacgggaa
    54541 gtacggtgat tgccgctcat gaaggcaaag ctccgcagca gatcatcatt atggcgcatc
    54601 tggatactta cgctccgctg agcgatgctg acgccgatgc caatctcggc gggctgacgt
    54661 tacaaggaat agatgataac gccgcaggtt taggtgtcat gctggaactg gcagaacgcc
    54721 tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagtggt gaagaggaag
    54781 ggaaattagg tgctgagaat ttactcaagc ggatgagtga caccgaaaag aaaaatacgc
    54841 tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc aatagtggtg
    54901 taaaaacccc tgaagcagta aggaaattaa cgcgcgacag ggcgctggca attgcgcgta
    54961 gtcatggaat tgccgcaacg accaatccgg gtttgaataa aaattatccg aaaggcactg
    55021 ggtgttgtaa tgacgcagaa atattcgaca aagccggtat tgctgtactt tcggtggaag
    55081 cgactaactg gaatcttggg aataaggatg gttatcagca acgcgcaaaa acagccgcat
    55141 tccctgcggg aaatagctgg catgacgtaa gactggataa tcaacaacat attgataagg
    55201 cgcttcctgg aagaatagaa cgtcgctgcc gtgatgttat gcggataatg ctacctctgg
    55261 tgaaggagtt ggcgaaggcg tcttgatggg tttgaaaatg ggagttggga gttctaccgc
    55321 aggggcgggg aattctaagt gatatccatc atcgcatcca gtgcgcccgg tttatccccg
    55381 ctgacgcggg gaacacacat gaatgtcggt tcagaccgtg tttttacccg gtttatcccc
    55441 gctggcgcgg ggaacacgag gctgaacagg gagggggcta ccggcacagc ggtttatccc
    55501 cgctggcgcg gggaacactt gccaaccgcc aaagactgga aaaccaatct cggtttatcc
    55561 ccgctggcgc ggggaacacg aactggcgtt gagtgataaa tttgacggaa tcggtttatc
    55621 cccgctggcg cggggaactc ttcagtttca cactcggtaa tgccgttatt tacggtttat
    55681 ccccgctggc gcggggaact cctcggggcc agcctccccc attaacccgg caccggttta
    55741 tccccgctgg cgcggggaac tctctaaaag tatacatttg ttcttaaagc attttttccc
    55801 ataaaaacaa cccaccaacc ttaatgtaac atttccttat tattaaagat cagctaattc
    55861 tttgttttta aacaggtaaa aaagagacca accttaaacc atccaaatct accggagtac
    55921 gcctgtttac cccaaatgtc tggaactcaa atcccgattc cgtattcgta gcccatgcca
    55981 tcaccacatt gccttcttcc gccagtccag atatttgttc ccagatcatt tcacggactc
    56041 ttgcggatac atcacctaca tacacccctg cacgtacctc caacaaccag atggctaatc
    56101 tgcctcgtaa gcgcgaaggt acattttcag tgaccacgac caacatactc atctcagcta
    56161 ctccgatgtc cggcatctcc cagagaaacg ggaagtggaa tagcaacagg ctgcgcatct
    56221 tcaggtgggg ccggcggctg tatttcccca gcagcaagca cgtcctctat aagcggaatc
    56281 aatttgacta atgttttact actgcgaaaa atatccctgc acgccaaacg gacttcccgg
    56341 tccggctcac caggaatacg acgcgctatc tcaaaagctt tcggtacaac agtgtcaaat
    56401 ttaatgatgt ccgcgatatc gtaaacaaag gaaagaggct ttcctgtatg cacaaaccca
    56461 atagctggtg cataaccagc tgcaagtatc gccgcttcag ttacgccata taaacaagaa
    56521 gttgcagcgc taatgcattg gttgaccgta tcgccctttt cccagtcttt cggatcgtaa
    56581 cgacgtccat tccatttcac cccgtattgc ttcgccagaa gtacataggt tgctcgcaca
    56641 cgacttcctt ctatacctct gagttgctct acggagcgcc gggcaggcgc aggttctcca
    56701 aaccgaagtt caaacatttt acgtacgacc ttcagacgca aatcttcatc cagagcaagt
    56761 tttgcctgat agagcagctt atctgaacgc gcaccaccag gctgaccaga agcataaaca
    56821 cgaacgcccg cttcccccac ccataccaat aatgttccaa cttgcgcagc caggcgtaca
    56881 gctgcatgcg aaacccgtgt accaggttcc aacatgatgc aggcaaccga gccaacagga
    56941 atatgagtgc ggatccctgt cttgtcgata agtacaaacg cgccatctat tacatcgatc
    57001 tgcccatatt gcagaaagat catggagacg cgatctttga gtggaatggg attaagggga
    57061 agccaggtca ttttattaca cctcaatcac agtggagcca aagatagcaa gccacatccc
    57121 atcgatttag ctggcccaat accttgctgt acaagatcta ttaacgctgg cgcgtcgttg
    57181 atggtgatca ccccttcaaa gcaaaccgtt tggatctttc cacttttacc atcaccagaa
    57241 aaatactgtg gccgttccga tatgggatgc acatcttcaa cgcgcgccgc attgcccaat
    57301 ttacgttgca accacgcgat ttgttctgct tcttttatta acggaacccg acagcgttta
    57361 atattccctt tactgtccag gcgcttttga ttgtcgagaa tagttttgat cggatttgcc
    57421 cgaagccgaa aatagagtgg aacaccaacc tgaagttgaa attcaacctg tttagtttta
    57481 atgactgtcg caacggcagt agaaacaggc atttgcgctg actgcaataa aacatgacag
    57541 ccttctggtg tgtttcgctt ctcaacatga aaaagaaaat cacgagcagc atccggtctg
    57601 tttggaaata aatgccataa tccctggtga agttggtaaa gatccctgct ccaggccctg
    57661 gcaatgatga ctttactgag atacatccat acctccttta atcacatacc attctcggga
    57721 agcaaattgt cgaggtaagg tgatcatcgg ttcgtcgcgc accgtaaatt ttaaatgatg
    57781 cccgtcaact gattcctcac tataaatatc gccgccaacg ggctcataat ttaatagcgc
    57841 cttctgagga tccgacgcct gacatgtccc gagaaaaagc ggttgtgtta gcggacaact
    57901 cctccgcccc agatacggtg tataccgagg ctttaatact gctttttcaa gttctgacat
    57961 aaccatcgtt gcctgtggtg ttaaccagat agcgacggta aaggaggcat cacataaata
    58021 ttcgcgccat gtttgaatcg tttcatgact tttcaaacca cggtaatctt ctcgcgctcc
    58081 aaggactgta tggtaatcac gcaaccccgt tacagacaca cgacgatcgt caagaatgag
    58141 ttcatcgcag cgcactgcaa attgcacact ctctgataac gcctgtaatg aagaagtatc
    58201 atcacgttgg atcccaagac aagccccgag tagccctaat aacccgcttc gggtcggaaa
    58261 tcttccggta ggtcgcgttc cttcaaaggt cggctgcccc caggcttgca ttggcccagc
    58321 aagccgcaag atcaaataag atctcatgtt cacgcctcgc cattattacg aacccaggat
    58381 tttaactgtt ctaaagtagg catttgttta acttgagcag taattgggtc tacatcagat
    58441 aagctgaatt gcgcagcagc tccgttcaga ccatatccat tggcaacgcg atcccaatat
    58501 tgattaaacg cctgtataga cggttgcaaa aagccatctt tcgctttaac cgctttttca
    58561 aaagcatttg ccatagaaag tggcatatcg gagaaattaa ccattaccat atccgcaggg
    58621 ttaaaagcgg cataagtacg ctgttttgct ccagggacct ctgttgccag catatgaaca
    58681 acatgggttg caatttccag agcctgctcc ctggaggcac cacctaaatt ttcctgaagt
    58741 tgagcgaggt taatgttggc ataacgataa aaaacacccg atgaaaattc ctgagttccc
    58801 agatgtgcag aaccttgttc ctgtaaatca tctacagcgg tgaaccagtc aatatcagaa
    58861 tcaacctgat gagtagtgat cgcatgcgca atggacattg caccatcaac ttttcccaac
    58921 tcactcatca tgccgctggt tgccattctt ccactaagcg caatatcaac accctgctgt
    58981 aaattcacac gtatggcggc aatatcttcc ttaagaactt tgagcagctt tttatcatcc
    59041 agattatcag cctctgcttt tgcaacctgc tcacagaacc aggctatttc tcccacaacc
    59101 cagggagtaa ccgcatcggc agaaatcttt tcggcttcat caactaattt accggagagc
    59161 agcgctaatg tcttatcgat gattttttgg tcaaaacgtt caccaagttt ttgccgaaga
    59221 acatcacgta attgtgcaag atgaattgtt ctgagactgg attcaccaat attttgtgcg
    59281 taataatcac ttttacgcat cgcatgttta aggctttgac ttgaaattct tactcgtctt
    59341 ttcccgccga aaatagcgtc tttctgcatg ttcatatcgt cgcggttcag acatgaaggg
    59401 ctgtgagaga tcagaacatg aatattgata aagttagaca tagaaaggtt tccttacgca
    59461 tttttgtttg tggtcaatac aaaatcttcc agaagttgct ggcgttcgcg ctttccccac
    59521 caggtcaaca tcctggccat taatggccag tcaagtacgg gttcggcgtg agtaagtaat
    59581 cgacgtaact ggaccatatc ggctgttctg tcagcccgaa ttaattgaaa gatacggcgc
    59641 tcgttaattc ttccactatt ggctaaagct cttcccaacg agatacctgt tgtttgctcc
    59701 gtttttttgt cctgatgtcg gatgacattc tttcctgcgc tcaggcaaaa caccatgcgc
    59761 aaaagagcct gctggtgacg tgggttttcc caaccaaaag attgcaccag cctataaaac
    59821 gcagggatat cgcgtaattc atcaggttct gaaacacgtc taatttgcgc acatgctcca
    59881 ttatccagtt gttgccaggc tcgatataaa gccattgcat caatttcatc agccatttga
    59941 tggccctcct tgaggtttta actcccgtaa atgtttgtat agcgtggcgc gggcaagcgc
    60001 taatgtgctt attaatttag gatgatgtgc atagggagct acagattgat taaatagcat
    60061 ttcacacaat tgatgaagtt tgtctcgtaa atcagctatt acctcatcag cttgggaaaa
    60121 attaatattc gccaatacat cgggaattaa taattcactc tgtcgataga aatgcctttc
    60181 tgcagtctca tgaacagaaa ctccggcccc tttgaagtct ttatttttaa acccttctgc
    60241 aaaggtatat aacgccttgc gtaaggctgt tttatatccc aaaccaacag tcactatttc
    60301 gtttatcaca ttgccgtatt gttgccaccc ctgattaaac atcaacacat catgacgccg
    60361 ttcaagaata gatgcttgat tattacgata tccccccata atcaattcaa gaggactttg
    60421 cggcgcaata tttctgaatt gattcacaac cgccgccacg cgatttccat tttcattttg
    60481 aataatctta tctaccacaa ctcggctgat ttgtgtccat gatggtgcgg aggtggtgaa
    60541 agcaagaaat ttttcctcaa cctccccttt cttgactgtt accagacaag gggaatgcgg
    60601 atggggccat agcccattaa ctgtaaaggt aaatttttcc ttaagaaaac cggtataacg
    60661 caaattgctt tcctgaccac agcaagaaca tttaccaatc ccaatgggat cgcatagttc
    60721 aatatgcgct ggttgccaga ataaaccacg gacaaaccca attgaagaag caggcaccga
    60781 ttcattcggc ttgacaggct taacccaggt aggttggttt tccgtatgta attcattggg
    60841 aaattgtttt tgaagacgag gtattgtgag gacattgagt aataccgtcg agcgaagatc
    60901 gatcccacat acgaaggtgg ttataggtgt tcctccacgt aaaccgcttt taaagccacc
    60961 accaaaacca ggtgcctgat tcgcctggtt gaataacgca atcgcagtgc atccaccaca
    61021 taatgcttca ccctgccccg gttgattgac aaatgcacaa ttcgtcgcgc cgcttacccc
    61081 agccaacagt ttttccattg gagtcacatc atttgctttg acacctttgg tctgcataaa
    61141 gggatgttct gcgtgattaa ggtagaacat atctatccac ggcgcgatga gttgttgaaa
    61201 ctcatcttca gtgagcggat tcattatgcg atgtcgaaat tcaacgtcat cttttgccgg
    61261 ggcgataatt tgcccaatgc aaaccagcag tgctaaagtg gccagttcca tatcgtcacg
    61321 gggcaaactt aatcgccact gatctctact gcagtatagc gattgcagat ttatgatttg
    61381 gactttcccc ccgtttcgcg ggcgtacagg gatccagtta tcaataagca aattcatttg
    61441 ttctccttca tatgctccga catttctcct gcatttctat gcgtcggcac acttccgtta
    61501 tcggtaaaaa caaagaaaaa atacggcgta attaattcaa taatcacatt cactgcaaaa
    61561 atatatt
//
LOCUS       DAZGTJ010000020        61436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000020 DAZGTJ010000000
VERSION     DAZGTJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.020"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            109..1047
                     /gene="tdcA"
                     /locus_tag="ACHK8E_001590"
     CDS             109..1047
                     /gene="tdcA"
                     /locus_tag="ACHK8E_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP0343877.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            1146..2135
                     /gene="tdcB"
                     /locus_tag="ACHK8E_001591"
                     /pseudo
     CDS             1146..2135
                     /gene="tdcB"
                     /locus_tag="ACHK8E_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
     gene            2157..3488
                     /gene="tdcC"
                     /locus_tag="ACHK8E_001592"
     CDS             2157..3488
                     /gene="tdcC"
                     /locus_tag="ACHK8E_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP0343878.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3514..4722
                     /gene="tdcD"
                     /locus_tag="ACHK8E_001593"
     CDS             3514..4722
                     /gene="tdcD"
                     /locus_tag="ACHK8E_001593"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP0343879.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4756..7050
                     /gene="tdcE"
                     /locus_tag="ACHK8E_001594"
     CDS             4756..7050
                     /gene="tdcE"
                     /locus_tag="ACHK8E_001594"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP0343880.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7064..7453
                     /gene="tdcF"
                     /locus_tag="ACHK8E_001595"
     CDS             7064..7453
                     /gene="tdcF"
                     /locus_tag="ACHK8E_001595"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP0343881.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7525..8889
                     /gene="tdcG"
                     /locus_tag="ACHK8E_001596"
     CDS             7525..8889
                     /gene="tdcG"
                     /locus_tag="ACHK8E_001596"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0343882.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9164..10495
                     /gene="cyuP"
                     /locus_tag="ACHK8E_001597"
     CDS             9164..10495
                     /gene="cyuP"
                     /locus_tag="ACHK8E_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0343883.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            10523..11833
                     /gene="cyuA"
                     /locus_tag="ACHK8E_001598"
     CDS             10523..11833
                     /gene="cyuA"
                     /locus_tag="ACHK8E_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP0343884.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11965..12129)
                     /gene="yhaL"
                     /locus_tag="ACHK8E_001599"
     CDS             complement(11965..12129)
                     /gene="yhaL"
                     /locus_tag="ACHK8E_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP0343885.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12152..12853)
                     /gene="yhaK"
                     /locus_tag="ACHK8E_001600"
     CDS             complement(12152..12853)
                     /gene="yhaK"
                     /locus_tag="ACHK8E_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP0343886.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12958..13854
                     /gene="yhaJ"
                     /locus_tag="ACHK8E_001601"
     CDS             12958..13854
                     /gene="yhaJ"
                     /locus_tag="ACHK8E_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0343887.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(13905..14261)
                     /locus_tag="ACHK8E_001602"
     CDS             complement(13905..14261)
                     /locus_tag="ACHK8E_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0343888.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(14504..14869)
                     /gene="yhaH"
                     /locus_tag="ACHK8E_001603"
     CDS             complement(14504..14869)
                     /gene="yhaH"
                     /locus_tag="ACHK8E_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0343889.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(15160..16146)
                     /gene="yqjG"
                     /locus_tag="ACHK8E_001604"
     CDS             complement(15160..16146)
                     /gene="yqjG"
                     /locus_tag="ACHK8E_001604"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP0343890.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(16216..16608)
                     /gene="yqjF"
                     /locus_tag="ACHK8E_001605"
     CDS             complement(16216..16608)
                     /gene="yqjF"
                     /locus_tag="ACHK8E_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0343891.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(16795..17094)
                     /gene="yqjK"
                     /locus_tag="ACHK8E_001606"
     CDS             complement(16795..17094)
                     /gene="yqjK"
                     /locus_tag="ACHK8E_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP0343892.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(17084..17488)
                     /gene="yqjE"
                     /locus_tag="ACHK8E_001607"
     CDS             complement(17084..17488)
                     /gene="yqjE"
                     /locus_tag="ACHK8E_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0343893.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(17491..17796)
                     /gene="yqjD"
                     /locus_tag="ACHK8E_001608"
     CDS             complement(17491..17796)
                     /gene="yqjD"
                     /locus_tag="ACHK8E_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP0343894.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(17834..18202)
                     /gene="yqjC"
                     /locus_tag="ACHK8E_001609"
     CDS             complement(17834..18202)
                     /gene="yqjC"
                     /locus_tag="ACHK8E_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP0343895.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(18349..18732)
                     /gene="mzrA"
                     /locus_tag="ACHK8E_001610"
     CDS             complement(18349..18732)
                     /gene="mzrA"
                     /locus_tag="ACHK8E_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP0343896.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(18736..19398)
                     /gene="yqjA"
                     /locus_tag="ACHK8E_001611"
     CDS             complement(18736..19398)
                     /gene="yqjA"
                     /locus_tag="ACHK8E_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP0343897.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(19420..19503)
                     /locus_tag="ACHK8E_001612"
     CDS             complement(19420..19503)
                     /locus_tag="ACHK8E_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343898.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(19743..20519)
                     /gene="exuR"
                     /locus_tag="ACHK8E_001613"
     CDS             complement(19743..20519)
                     /gene="exuR"
                     /locus_tag="ACHK8E_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP0343899.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(20649..21947)
                     /gene="exuT"
                     /locus_tag="ACHK8E_001614"
     CDS             complement(20649..21947)
                     /gene="exuT"
                     /locus_tag="ACHK8E_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP0343900.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            22430..23842
                     /gene="uxaC"
                     /locus_tag="ACHK8E_001615"
     CDS             22430..23842
                     /gene="uxaC"
                     /locus_tag="ACHK8E_001615"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP0343901.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            23857..25344
                     /gene="uxaA"
                     /locus_tag="ACHK8E_001616"
     CDS             23857..25344
                     /gene="uxaA"
                     /locus_tag="ACHK8E_001616"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP0343902.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            25427..25978
                     /gene="ygjV"
                     /locus_tag="ACHK8E_001617"
     CDS             25427..25978
                     /gene="ygjV"
                     /locus_tag="ACHK8E_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP0343903.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(25983..27227)
                     /gene="sstT"
                     /locus_tag="ACHK8E_001618"
     CDS             complement(25983..27227)
                     /gene="sstT"
                     /locus_tag="ACHK8E_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP0343904.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(27432..27734)
                     /locus_tag="ACHK8E_001619"
     CDS             complement(27432..27734)
                     /locus_tag="ACHK8E_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343905.1"
                     /translation="MASKSPKRSLEAGATRMDARRGRILQGRSLAPDPVAARDKLREP
                     RSGDFVRQSLGVQGAAAIGRPLRAPCASGFIWHSTLKERNRSHIPNSFAHMLSNQP"
     gene            complement(27843..28808)
                     /gene="alx"
                     /locus_tag="ACHK8E_001620"
     CDS             complement(27843..28808)
                     /gene="alx"
                     /locus_tag="ACHK8E_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP0343906.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(29091..30077)
                     /gene="ygjR"
                     /locus_tag="ACHK8E_001621"
     CDS             complement(29091..30077)
                     /gene="ygjR"
                     /locus_tag="ACHK8E_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0343907.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(30156..30839)
                     /gene="ygjQ"
                     /locus_tag="ACHK8E_001622"
     CDS             complement(30156..30839)
                     /gene="ygjQ"
                     /locus_tag="ACHK8E_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP0343908.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(30916..31419)
                     /gene="ygjP"
                     /locus_tag="ACHK8E_001623"
     CDS             complement(30916..31419)
                     /gene="ygjP"
                     /locus_tag="ACHK8E_001623"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP0343909.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            31504..32640
                     /gene="rlmG"
                     /locus_tag="ACHK8E_001624"
     CDS             31504..32640
                     /gene="rlmG"
                     /locus_tag="ACHK8E_001624"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP0343910.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            32831..32914
                     /locus_tag="ACHK8E_001625"
                     /pseudo
     CDS             32831..32914
                     /locus_tag="ACHK8E_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            32911..33327
                     /gene="higA"
                     /locus_tag="ACHK8E_001626"
     CDS             32911..33327
                     /gene="higA"
                     /locus_tag="ACHK8E_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP0343911.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(33372..35390)
                     /gene="fadH"
                     /locus_tag="ACHK8E_001627"
     CDS             complement(33372..35390)
                     /gene="fadH"
                     /locus_tag="ACHK8E_001627"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP0343912.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(35561..37912)
                     /gene="ygjK"
                     /locus_tag="ACHK8E_001628"
     CDS             complement(35561..37912)
                     /gene="ygjK"
                     /locus_tag="ACHK8E_001628"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP0343913.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(37929..38999)
                     /gene="ygjJ"
                     /locus_tag="ACHK8E_001629"
     CDS             complement(37929..38999)
                     /gene="ygjJ"
                     /locus_tag="ACHK8E_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP0343914.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(39133..40566)
                     /gene="ygjI"
                     /locus_tag="ACHK8E_001630"
     CDS             complement(39133..40566)
                     /gene="ygjI"
                     /locus_tag="ACHK8E_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0343915.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(40629..41078)
                     /gene="ebgC"
                     /locus_tag="ACHK8E_001631"
     CDS             complement(40629..41078)
                     /gene="ebgC"
                     /locus_tag="ACHK8E_001631"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP0343916.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(41075..44167)
                     /gene="ebgA"
                     /locus_tag="ACHK8E_001632"
     CDS             complement(41075..44167)
                     /gene="ebgA"
                     /locus_tag="ACHK8E_001632"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP0343917.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(44351..45334)
                     /gene="ebgR"
                     /locus_tag="ACHK8E_001633"
     CDS             complement(44351..45334)
                     /gene="ebgR"
                     /locus_tag="ACHK8E_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP0343918.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            45553..45885
                     /gene="ygjH"
                     /locus_tag="ACHK8E_001634"
     CDS             45553..45885
                     /gene="ygjH"
                     /locus_tag="ACHK8E_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP0343919.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(45927..47306)
                     /gene="ygjG"
                     /locus_tag="ACHK8E_001635"
     CDS             complement(45927..47306)
                     /gene="ygjG"
                     /locus_tag="ACHK8E_001635"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP0343920.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
     gene            47724..49244
                     /gene="aer"
                     /locus_tag="ACHK8E_001636"
     CDS             47724..49244
                     /gene="aer"
                     /locus_tag="ACHK8E_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP0343921.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(49348..49971)
                     /gene="nfeR"
                     /locus_tag="ACHK8E_001637"
                     /gene_synonym="yqjI"
     CDS             complement(49348..49971)
                     /gene="nfeR"
                     /locus_tag="ACHK8E_001637"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP0343922.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            50260..51024
                     /gene="nfeF"
                     /locus_tag="ACHK8E_001638"
                     /gene_synonym="yqjH"
     CDS             50260..51024
                     /gene="nfeF"
                     /locus_tag="ACHK8E_001638"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP0343923.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            51321..52637
                     /locus_tag="ACHK8E_001639"
     CDS             51321..52637
                     /locus_tag="ACHK8E_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0343924.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRLCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            52767..53363
                     /locus_tag="ACHK8E_001640"
     CDS             52767..53363
                     /locus_tag="ACHK8E_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343925.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(53456..55069)
                     /locus_tag="ACHK8E_001641"
     CDS             complement(53456..55069)
                     /locus_tag="ACHK8E_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343926.1"
                     /translation="MVNQTMQQNQYYRILSGESRPFPGIYEAVSEQLLLLIFKARQLY
                     QDPEYRPWFEEQHKVLLALRDNTELQKIFNKQRKENFALGASTSSQPTALTNALNVMC
                     HLYNGFDEGNISLKSWGAMQEVQTTPDFFSLGPRTWKTPELAKFVGDDLFNILTECKA
                     WSSPIPAEARQGYCHQDLSVAHSCTSELLAHLKQYHEIDESTILAITIGLAVNEFYQY
                     KQNIPEWLSCFENKNKWPWDCRKSDTEDVHRLRERLSIIGCTEFVKNMFSVASQHEIK
                     TMYNIYVHIYAAEICGWVKTSMAYFKPTDFIQYTWKFATVTPDKDEKTENTTAWERDY
                     CQITIQRDELLRLIRTNTLQDAAIHLSGCLQYAYLESVKNDIDYPGIAPNAEEKKMKY
                     TDAIEFSGIRNNIINAVKRLESFEKWYETNKVLLVSNKTIRTEKIDVVVRLAGLKAYD
                     LQEGVPDGLKWKVKDGINERIKTDTSLCLPQDISDTSLNRYRHAVTRIVNDEIDTLLM
                     EQKKKNKRFPYSKDRHAIQPLWSKCVIEQ"
     gene            55799..56026
                     /locus_tag="ACHK8E_001642"
     CDS             55799..56026
                     /locus_tag="ACHK8E_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0343927.1"
                     /translation="MWVPLTDEQRSVILKAYGTEGDRLVREKERKEITSISRSQAWKL
                     EREGNYPPRKTIGKKSCGWLLSDLLWWIQTR"
     gene            56122..57555
                     /locus_tag="ACHK8E_001643"
     CDS             56122..57555
                     /locus_tag="ACHK8E_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0343928.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQQNTQAPLAMVATSALTA
                     MSIACQNQIDVCRPGNLRGPVNIYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNNSPVPPQIRRTI
                     FNDTTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSMQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYMRLSNPL
                     TFDNTALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            58472..59065
                     /locus_tag="ACHK8E_001644"
     CDS             58472..59065
                     /locus_tag="ACHK8E_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0343929.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            59436..59819
                     /locus_tag="ACHK8E_001645"
     CDS             59436..59819
                     /locus_tag="ACHK8E_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0343930.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            59816..60241
                     /locus_tag="ACHK8E_001646"
                     /pseudo
     CDS             59816..60241
                     /locus_tag="ACHK8E_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            60713..61279
                     /locus_tag="ACHK8E_001647"
     CDS             60713..61279
                     /locus_tag="ACHK8E_001647"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0343931.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGTIERHLGCSDF"
BASE COUNT        15170 a        15060 c        15770 g        15436 t
ORIGIN      
        1 gataccctgg aaagtattga aagttaattt gtgagtggtc gcacatatcc tgttcatttc
       61 attttgatac acttcatgcc gtcaatgagg taattaacgt aggtcgttat gagcactatt
      121 cttcttccga aaacgcagca cctggtagtc tttcaggaag ttattagaag tggttctatc
      181 ggctcggctg caaaagagtt agggttaact caaccggccg tcagtaaaat cattaacgat
      241 attgaagatt attttggtgt ggagttagtg gtgcggaaaa ataccggtgt aacattaaca
      301 cctgccggtc aattgttact ctcccgttcc gaatccatta cccgtgaaat gaaaaatatg
      361 gttaatgaga taagcggtat gtcttctgag gcggtggtgg aagtctcatt tggttttcct
      421 tcattgattg gttttacttt tatgtcaggg atgatcaaca agttcaaaga ggtgttcccg
      481 aaagcgcagg tttctatgta tgaagcgcaa ctgtcttcgt tcttaccggc aatccgcgac
      541 ggtcgactgg attttgcgat tggtacgtta agcgcagaaa tgaagcttca ggatttacat
      601 gttgagccgc tgttcgagtc cgagtttgtg ctggtagcca gtaagtcccg aacatgcacc
      661 ggcaccacca cgctggagtc gttgaagaac gaacagtggg tgttgccaca aaccaatatg
      721 gggtactaca gcgaactact tactacgtta caaagaaatg gcatcagtat tgaaaacatc
      781 gttaaaaccg actcagtcgt gacaatttat aatcttgttc tcaatgctga tttcttaact
      841 gtaattcctt gtgatatgac atcacctttt ggttctaatc aatttattac tattccggtt
      901 gaagaaacat tacctgtggc acaatatgcc gcggtatggt cgaaaaatta tcgtattaaa
      961 aaagcagcat cggttttggt ggaattagcc aaagagtatt catcttataa tgggtgtaga
     1021 cgaaggcaat taattgaagt tgattagtta tttgttttaa tttaaacata aataatccac
     1081 ctgtctgttt tgcaggtgtc ggttacggtt acctacatat ttaattcagg ctaagaggtt
     1141 ttataatgca tattacatac gatctcccgg ttgctattga tgacatcatt gaagcaaaac
     1201 aacgactggc tgggtgaatt tataaaacag gtatgcctcg ctccaactat tttagtgaac
     1261 gttgcaaagg tgaaatattc ctcaaattcg aaaacatgca gcgtaccggt tcatttaaaa
     1321 ttcgtggcgc atttaataaa ttaagttcac tgactgatgc ggaaaaacgt aaaggtgtag
     1381 tggcctgctc tgcgggcaac catgcgcaag gggtttcgct ctcctgtgcg atgctgggta
     1441 ttgacggtaa agtggtgatg ccaaaaggtg cgccgaaatc caaagtcgcg gcaacgtgcg
     1501 actactccgc agaagtcgtt ttgcatggtg ataacttcaa cgacactatt gctaaagtga
     1561 gcgaaattgt cgaaatggaa ggccgtattt ttatcccacc ttacgatgat ccgaaagtga
     1621 ttgctggcca gggaacgatt ggtctggaaa ttatggaaga tctctatgat gtcgataatg
     1681 tgattgtgcc aattggcggt ggcggtttaa ttgctggtat tgcggtagcg attaaatcaa
     1741 ttaacccgac tattcgtgtt attggcgtac agtcagaaaa cgttcacggc atggcggctt
     1801 ctttccactc cggagaaata actacgcacc gaactaccgg caccctggcg gatggttgtg
     1861 atgtctcccg cccgggtaat ttaacttacg aaatcgttcg tgaattagtc gatgacatcg
     1921 tgctggtcag cgaagacgaa atcagaaaca gtatgattgc cttaattcag cgcaataaag
     1981 tcgtcaccga aggcgcaggc gctctggcat gtgctgcatt attaagcggt aaattagacc
     2041 aatatattca aaacagaaaa accgtcagta ttatttccgg cggcaatatc gatctttctc
     2101 gcgtctctca aatcaccggt ttcgttgacg cttaattaat tcgttgagga taggatatga
     2161 gtacttcaga tagcattgta tccagccaga caaaacaatc gtcctggcgt aaatcagata
     2221 ccacatggac gttaggcttg tttggtacgg caatcggcgc cggggtgctg ttcttcccta
     2281 tccgcgcagg ttttggcgga ctgatcccga ttcttctgat gttggtatta gcatacccca
     2341 tcgcgtttta ttgccaccgg gcactggcgc gtctgtgtct ttctggctct aacccttccg
     2401 gcaacattac ggaaacggtg gaagagcatt ttggtaaaac tggcggcgtg gttatcacgt
     2461 tcctgtactt cttcgcgatt tgcccactgc tgtggattta tggcgttact attaccaata
     2521 cctttatgac gttctgggaa aaccagctcg gctttgcacc gctgaatcgc ggctttgtgg
     2581 cgctgttcct gttgctgctg atggctttcg tcatctggtt tggtaaggat ctgatggtta
     2641 aagtgatgag ctacctggta tggccgttta tcgccagcct ggtgctgatt tctctgtcgc
     2701 tgatccctta ctggaactct gcagttatcg accaggttga cctcggttcg ctgtcgttaa
     2761 ccggtcatga cggtatcctg atcactgtct ggctggggat ttccatcatg gttttctcct
     2821 ttaacttctc gccaatcgtc tcttccttcg tggtttctaa acgtgaagag tatgagaaag
     2881 acttcggtcg cgacttcacc gaacgtaaat gttcccaaat catttctcgt gccagcatgc
     2941 tgatggttgc agtggtgatg ttctttgcct ttagctgcct gtttacgctg tctccggcca
     3001 acatggcgga agccaaagcg cagaatattc cagtgctttc ttatctggct aaccactttg
     3061 cgtccatgac cggtaccaaa acaacgttcg cgattacact ggaatatgcg gcttccatca
     3121 tcgcactcgt ggctatcttc aaatctttct tcggtcacta tctggggacg ctggaaggct
     3181 tgaatggcct gattctgaag ttcggttata aaggtgacaa aaccaaagtg tcgctgggta
     3241 aactgaatac tctcagcatg atcttcatca tgggctccac ctgggttgtt gcctacgcca
     3301 acccgaacat cctcgacctg attgaagcca tgggcgcacc gattatcgca tcactgctat
     3361 gcctgttgcc gatgtatgcc attcgtaaag cgccgtctct ggcgaaatac cgtggtcgtc
     3421 tggataacgt gtttgttacc gtgattggtc tgctgactat cctgaacatc gtatacaaac
     3481 tgttttaatc cgtaactcag gatgagaaaa gagatgaatg aatttccggt tgttttggtt
     3541 attaactgtg gttcgtcttc gattaagttt tccgtactca atgccagcga ctgtgaagta
     3601 ttaatgtcag gtattgccga cggtattaac tcggaaaatg cattcttatc cgtaaatggg
     3661 ggagagccag caccgctggc tcaccacagc tacgaaggtg cattgaaggc aattgcattt
     3721 gaactggaaa aacggaattt aaatgacagt gtggccttaa ttggccaccg catcgctcac
     3781 ggcggcagta tttttaccga gtccgccatt attaccgatg aagtcattga taatatccgt
     3841 cgcgtttctc cactggcacc cctgcataat tacgccaatt taagtggtat tgaatcggcg
     3901 cagcaattat ttccgggcgt aactcaggta gcggtatttg ataccagttt ccaccagacg
     3961 atggctccgg aagcttattt atacggcctg ccgtggaaat attatgaaga gttaggtgta
     4021 cgccgttatg gtttccacgg cacgtcgcac cgctatgttt cccagcgcgc gcattctttg
     4081 ctgaatctgg cggaagatga ctccggcctg gttgtggcgc atcttggcaa tggcgcgtca
     4141 atctgcgcag ttcgcaacgg tcagagtgtt gatacctcaa tgggaatgac gccgctggaa
     4201 ggcttgatga tgggtacccg cagtggtgat gtcgacttcg gggcgatgtc ctgggtcgcc
     4261 agccaaacca accagagcct gggtgacctg gaacgcgtcg tgaataaaga gtcgggatta
     4321 ttaggtattt ccggcctttc ttcggattta cgtgttctgg aaaaagcctg gcatgaaggt
     4381 cacgaacgcg cgcaattggc aattaaaacc tttgttcatc gaattgcccg tcatattgcc
     4441 ggacacgctg cttcattaca tcgcctggat ggaattatat tcaccggcgg aataggagag
     4501 aattcaagtt taattcgtcg tctggtcatg gaacatttag ctgtattagg cgtagagatt
     4561 gatacagaaa tgaataatcg ctctaactct tttggagagc gaattgtttc cagtgaaaat
     4621 gcgcgtgtca tttgcgccgt aattccgact aacgaagaaa aaatgattgc tttggatgcc
     4681 attcatttag gcaaagtgaa cgcgcccgca gaatttgcat aatttagttg aagtattgta
     4741 gagagattat ttttcatgaa ggtagatatt gataccagcg ataagctgta cgccgacgca
     4801 tggcttggct ttaaaggtac ggactggaaa aacgaaatta atgtccgcga ttttattcaa
     4861 cataactata caccgtatga aggtgatgaa tcttttctcg ccgaagcgac gcctgccacc
     4921 acggaattgt gggaaaaagt gatggaaggc atccgtatcg aaaatgcgac tcacgcgcca
     4981 ttggatttcg ataccaatat tgccaccaca attaccgctc atgatgcagg atatattgac
     5041 cagccgctgg aaaaaatagt gggtctgcaa acggatgcgc cattaaagcg tgcgctgcat
     5101 ccgttcggcg gcattaatat gattagaagt tcattccacg cgtatggtcg tgaaatggat
     5161 agcgaatttg aatacatctt taccgatctg cgtaaaaccc ataaccaggg cgtatttgat
     5221 gtttactcac cggatatgct gcgctgccgt aaatctggtg tactgaccgg tttaccagat
     5281 ggttatggtc gtgggcgtat catcggtgac taccgccgcg tggcgcttta cggcatcagt
     5341 tatctggtac gtgaacgcga actgcagttt gccgatctcc agtcccgtct ggaaaaaggc
     5401 gaagatctcg aagctacgat ccgtctgcgt gaggagctgg cggaacatcg tcgtgcgctg
     5461 ttgcagattc aagaaatggc ggcgaaatac ggttttgaca tctcccgtcc ggcgcagaat
     5521 gcacaggaag cggtgcagtg gctctacttc gcttacctgg cggcagtgaa atcgcaaaat
     5581 ggcggcgcga tgtcgctggg gcgcacggca tcgttcctcg atatctacat tgagcgcgac
     5641 tttaaagcag gcgtactcaa tgagcagcag gcacaggaac tgatcgacca cttcatcatg
     5701 aagatccgta tggtgcgctt cttgcgtacg ccggaatttg attcgctgtt ctccggcgac
     5761 ccgatctggg cgacggaagt gatcggcggg atggggctgg acggtcgaac cctggtgacc
     5821 aaaaactcct tccgctattt acacaccctg cacactatgg ggcctgcacc ggaacctaac
     5881 ctgactattc tgtggtcgga agccttgccg attgccttca aaaaatatgc cgcgcaggtg
     5941 tctatcgtca catcctcttt gcagtatgaa aacgacgatc tgatgcgtac tgacttcaat
     6001 agcgacgatt acgcgattgc ctgttgcgtc agcccaatgg tgattggtaa gcagatgcag
     6061 ttcttcggtg cgcgcgctaa cctggcgaaa acgctactgt acgcaatcaa cggcggaatg
     6121 gacgagaagc tgaagattca ggttggcccg aaaacggctc cgttgatgga tgacgtgctg
     6181 gactacgaca aagtgatgga tagcctcgac cacttcatgg actggctggc ggtacagtac
     6241 atcagcgcgc tgaatatcat ccactacatg cacgacaagt acagctatga agcctcgctg
     6301 atggcgctac acgatcgtga tgtctatcgc accatggcgt gcggcatcgc gggcctgtcg
     6361 gtggcaacgg actccctgtc tgccatcaaa tatgctcgtg tgaaaccaat ccgtgacgaa
     6421 aacggcctgg cggtggattt cgaaatcgac ggcgaatatc cgcagtatgg caacaacgac
     6481 gagcgcgtag acagcattgc ctgcgatctg gttgaacgct ttatgaagaa aattaaagct
     6541 ctgccaacct atcgcaatgc tgtgccgact caatcgattc tgactatcac ctctaacgtg
     6601 gtgtacggtc agaaaaccgg gaacacgccg gacggtcgcc gcgccggaac accgttcgcg
     6661 ccgggcgcta acccgatgca cggtcgtgat cgcaaaggcg ccgtggcctc attgacgtcg
     6721 gtggcgaaac tgccgttcac ctacgccaaa gatgggatct cgtacacctt ctccatcgtc
     6781 cctgcggcgc tgggcaaaga agatccagta cgtaaaacca accttgtcgg cctgctggat
     6841 gggtatttcc accacgaagc ggatgtcgaa ggcggtcaac acctcaacgt caacgtaatg
     6901 aatcgggaaa tgctgctgga tgccatcgag cacccggaaa aatatcctaa cctgacaatc
     6961 cgtgtctctg gctacgccgt gcgcttcaac gcactgaccc gtgaacagca acaggatgtt
     7021 atttcacgta cctttaccca ggcgctctga cgccggagga tgtatgaaaa agattatcga
     7081 aacgcaacgt gccccaggcg caatcggccc ttatgttcag ggcgttgatt taggcagcat
     7141 ggtcttcacc tccgggcaaa ttccggtttg cccacagacc ggtgagatcc cggctgatgt
     7201 gcaagatcag gcgcgtttaa gcctcgaaaa cgtcaaagcg atcgtggttg ctgccgggct
     7261 gagtgtgggc gatatcatca agatgaccgt gttcatcacc gatctgaatg attttgccac
     7321 catcaatgaa gtctataagc agttcttcga tgaacatcag gcgacctacc cgacccggag
     7381 ctgtgtgcag gtcgcgcgtt tgccgaaaga tgtgaagttg gaaattgaag ccatcgcagt
     7441 acgtagtgcg taaggcctcg tgagcgggac ggtcgcaagg tcgttccgct ccacttcact
     7501 gaacggcaat ccgagggtgt ggatatgatt agtgcattcg atattttcaa aattgggatt
     7561 ggtccctcca gttcgcatac tgtggggcca atgaatgccg gaaaaagttt tattgatcgg
     7621 ctggaaagta gcggcttatt aaccgcgacg agccatattg tggtcgatct gtacgggtcg
     7681 ttgtcactga cgggcaaagg ccatgccacg gatgtcgcca tcatcatggg actggcagga
     7741 aacagtccgc aggatgttgt cattgatgag atccctgcat ttatagagtt agtgacgcgc
     7801 agcgggcggt tgccggtggc ttctggcgcg catattgttg attttcctgt agcaaagaac
     7861 attatcttcc atcccgaaat gttgcctcgc catgagaacg gaatgcggat cactgcctgg
     7921 aagggacagg aagagctatt aagcaaaacc tattactctg tcggcggcgg gtttattgtc
     7981 gaagaagaac acttcggcct gtcgcacgat gtcgaaacac ccgtacctta cgatttccac
     8041 tcagcaggtg aactgctgaa aatgtgtgat tacaacggcc tgtctatatc tggcctgatg
     8101 atgcacaacg agctggcgct gcgcagtaaa gcggaaattg acgcagggtt tgcccgtatc
     8161 tggcaagtga tgcatgacgg tattgaacgc gggatgaaca ctgaaggcgt gctgcctggt
     8221 ccgctcaatg tgccgcgtcg tgccgtagcg ctgcgtcgtc aactggtttc cagcgataac
     8281 atctctaacg atccgatgaa tgtcatcgac tggatcaata tgtacgcgct ggcggttagt
     8341 gaagaaaacg cagctggcgg gcgcgtggta acggcaccga ctaacggtgc gtgcggcatt
     8401 attccggcag tgctggctta ttacgataag ttccgtcgtc cggtaaacga gcggtcaatt
     8461 gcccgctatt ttcttgcagc gggggctatt ggcgcgctgt ataaaatgaa cgcctccatc
     8521 tctggtgcgg aggtcggctg tcagggggag attggcgtgg cctgttcaat ggcggcggca
     8581 gggttaactg aactcctggg cggcagtccg gcgcaggtat gcaatgcggc ggaaatcgcg
     8641 atggagcata accttgggct gacctgcgat ccggttgccg gacaggtaca aatcccgtgc
     8701 attgaacgta atgccattaa tgccgtgaaa gcagtaaacg ccgcgcggat ggcgatgcgc
     8761 cgcacctcgg caccgcgtgt ttcactcgat aaagtgatcg agacgatgta tgaaaccggc
     8821 aaagatatga acgataaata ccgcgaaacg tcacgcggag gactggccat taaagtggtc
     8881 tgtggctgac attgctgtca gctttcatcc ttgggtgcac aaatgtgcac cttttttttg
     8941 tgatctgccg caaatagaga atgaatattc tcttgcgatt aatttagtag aaaattctta
     9001 tccatctccg ttatttcaaa gtcttgattg tgaagatttt ctctgacctc gcgcgtatca
     9061 tttctttatt ctaataactc gcctgcaaaa acgtagaccg catatcgcct tgatgcggct
     9121 cggaaaaaaa taataagccc tacaaaatat tgagcgagaa attatggaaa ttgcatcgaa
     9181 taaaggcgtc attgcagacg cttcgacccc ggcgggtcgt gctggaatga gtgagagcga
     9241 gtggcgagaa gcgatcaaat tcgacagtac tgacaccggc tgggtgatta tgagtatcgg
     9301 gatggcgatt ggcgcgggga ttgtttttct cccggtgcag gtcggtttga tgggattgtg
     9361 ggtatttttg ctctcatcgg tgattggtta cccggcaatg tatctgtttc agcggttgtt
     9421 tattaatacg ctggcagaat caccggagtg taaagattac ccgagcgtca tcagcggtta
     9481 tttaggtaaa aactggggca tcctgttagg tgcgctctat ttcgtaatgc tggtgatttg
     9541 gatgttcgtc tattccaccg ccatcaccaa cgatagtgct tcctacctgc ataccttcgg
     9601 cgtgacggaa ggggtgctgt cagacagtcc cttttatggt ctggtactga tttgcattct
     9661 ggtggcgatc tcctcacgcg gcgagaaatt gttatttaaa atttcgaccg gcatggtgct
     9721 gaccaagctg ctggtggtcg cggcgctggg cgtgtcgatg gtgggaatgt ggcatctgta
     9781 taacgtcggt tcgctaccgc cgctggggct gctggtgaaa aacgccatta ttacgctgcc
     9841 gtttaccctg acgtcgattc tgtttatcca gacgttaagc ccgatggtga tctcttatcg
     9901 ctcgcgggaa aaatcaattg aagtggcgcg gcataaagca ttgcgggcaa tgaatatcgc
     9961 gtttggcatt ttgtttgtca ccgtcttttt ctacgccgtg tcgttcacgc tggcgatggg
    10021 acatgacgaa gcggtaaaag cctacgagca gaatatttcc gcgttggcga ttgccgcaca
    10081 gtttattagc ggtgatggcg cagcgtgggt gaaagtggtc agcgtcattc tcaatatctt
    10141 tgctgtaatg actgcattct ttggcgtcta tttaggcttt cgcgaagcaa cgcaagggat
    10201 cgtaatgaac atcctgcgtc gcaagatgcc tgccgagaag attaacgaaa atctcgttca
    10261 gcgcggcatc atgattttcg ccattttgct ggcctggagc gccatcgtac tgaacgcacc
    10321 ggtgttgagc ttcacctcta tctgtagccc gattttcggc atggtagggt gcctgatccc
    10381 ggcgtggctg gtttacaaag taccggcatt gcacaaatac aaagggatgt ctctgtacct
    10441 gattatcgtc actggtttgt tgctttgtgt ttctccgttc ctggcatttt cttgatttac
    10501 ctgaaatttt aaggttttta atatgtttga ttcgacttta aatccgttat ggcagcgtta
    10561 catcctcgcc gttcaggagg aagtaaaacc ggcgctggga tgtactgaac cgatttcact
    10621 ggcgctggcg gcggcggttg ctgcggcaga actggaaggt ccggttgaac gtgtagaagc
    10681 ctgggtttcg ccaaatctga tgaagaacgg tctgggcgtc accgttcccg gcacgggaat
    10741 ggtggggctg ccaattgcgg cggcgctggg agcgttaggt ggaaatgcta acgccgggct
    10801 ggaagtgctg aaagacgcaa ctgcgcaggc aattgccgat gccaaagcac tgctggcggc
    10861 ggggaaagtc tcagttaaga tccaggaacc ttgcgatgaa atcctcttct cacgcgccaa
    10921 agtctggaac ggtgagaagt gggcgtgtgt caccatcgtc ggcgggcata ccaacattgt
    10981 gcatattgag acgcacaatg gtgtggtgtt tacccagcag gcgtgtgtga cagagggcga
    11041 gcaagagtcg ccgctgacgg tgctttcaag aacgacgctg gctgagatcc tgaagttcgt
    11101 caatgaagtc ccgtttgcgg cgatccgctt tattctcgat tccgcgaagc taaattgtgc
    11161 gttatcgcag gaaggtttga gcggtaagtg ggggctgcat attggcgcga cgctggaaaa
    11221 acagtgtgca cgcggtttgc tggcgaaaga tctctcttca tccattgtga ttcgtaccag
    11281 cgcggcatcc gatgcgcgta tgggcggcgc tacgcttccg gcaatgagta actccggctc
    11341 gggtaaccag gggatcactg caacaatgcc cgtggtggtg gtagcagaac acttcggggc
    11401 cgatgatgaa cggctggcgc gtgcgctgat gctttcgcat ttgagcgcaa tttacatcca
    11461 taaccagtta ccgcgtttgt ctgcgctgtg tgccgcaacg accgcagcaa tgggggccgc
    11521 cgccgggatg gcatggctgg tggatgggcg ttatgaaatc atttcgatgg cgatcagcag
    11581 tatgatcggc gatgtcagcg gcatgatttg cgatggagcg tcgaacagct gcgcgatgaa
    11641 ggtttcgacc agtgcttcgg ctgcgtggaa agcggtgtta atggcgctgg atgataccgc
    11701 cgtgaccggc aatgagggga tcgtggcgca tgatgttgag cagtcgattg ccaacctgtg
    11761 tgcgttagca agccattcga tgcagcaaac ggatcggcag attatcgaga ttatggcgag
    11821 caaggccaga taaacgccag ttttgggtat cgtgccggat gcgatgctgg cgcgtcttat
    11881 ctggcctacg aattgcacaa tttgttgatg ttgttgggtt ttgtaggccg gataaggcgt
    11941 ttacgccgca tccggcacct gtctttacgc ggtagcttca tcctcggcta aacgcgtttc
    12001 agcatccgcg acgatggctt ccagttcgct caacatctct tcatagccaa aatttttggc
    12061 ggtacgagca ggttctttcg ccaccacggg cttcactggc tggcgctttt tggccgattt
    12121 tttactcatc atcatctccc tgtaaaaaca actagacagg caaatctatc agcaaagcgc
    12181 gcagtgggga atcggcaacc agcgtaatgt tagcctcgtc acgaataaac gccccatcac
    12241 cgcaggttag cgccgctttc tcttcatgat gcgtaagcgc atgaaatttc ccgtgaatcg
    12301 attgcaaata cgcgcgtggt ccatgcaact ggaaattcgc actttcgcct ttgtcgagca
    12361 cgatatggtg cagccacact tgctggcgca gttgcaggct acccactgtg ccctctggcg
    12421 aggcgattaa ttgctgcttg cccatattaa gcgccagttt ttgaatcagc ggattctctc
    12481 gctgcgggca ggcatccagc caaagctgca ttcgcgttaa cggtttgtct ttgctgagat
    12541 tgtgttcgct atagctgaca cccggctggg tagagagcag caacgcctca ccagcgctgg
    12601 cctgaacatg attgccttcg ctgtcgcgat actctgcttc tccatccaga atcacattta
    12661 aaatatcgac tttgggatag gttcgcggct gaaaggcggc acctggggcc agcacttcct
    12721 ggttaagcac gcgcagggag gcatagccta acaatttcgg gtcgaagtag tgtccaaagg
    12781 aaaaagtata ccgggcctgc aaccatccgt agtctgcttg tccacactgc ctggcagttc
    12841 gggtagtaat cataattctt gacctctctt tacttccttt tatggtaagg ggctggacgc
    12901 tacattgtta gccagatatt ctgcccggta tgttcaaatt tcctgaatga gaacgaaatg
    12961 gccaaagaaa gggcattaac gctggaagca ctacgggtta tggatgcgat cgatcgccgg
    13021 ggcagttttg cggcggcggc ggatgagctg ggacgcgtgc cttccgcact tagctacacc
    13081 atgcaaaaac tggaagaaga gctggatgtg gtgctgtttg accgctcggg ccatcgcacc
    13141 aaattcacca atgtcgggag gatgttgctg gagcgaggac gcgttttgct ggaagccgca
    13201 gataaactga ctaccgatgc ggaagctctc gcgcgcggtt gggaaacgca tctcaccatt
    13261 gtgaccgaag cgctggtgcc gacacctgcc tttttcccgt taattgacaa actggcggca
    13321 aaagcgaata cccaactggc aatcatcacc gaagtgctgg cgggggcatg ggaacggctg
    13381 gagcaggggc gggcggatat tgttatcgcg ccggatatgc attttcgctc ctcgtcggag
    13441 atcaactcgc gcaagctcta tacgttaatg aacgtctacg ttgccgcgcc tgatcacccg
    13501 attcatcagg agccggaacc gttatctgaa gtgacgcgcg tgaaatatcg tggaattgcg
    13561 gtggcggata ccgctcgtga gcgcccggta ttgaccgtac agctactgga caaacagccg
    13621 cgtttaacgg tgagcacgat tgaagataaa cgtcaggcgt tactggcggg gcttggcgtg
    13681 gcgacgatgc cgtatccgat ggtcgaaaaa gacatcgcgg aagggcggtt gcgtgtcgtc
    13741 agcccggaat cgaccagcga gatcgatatt attatggcct ggcgtcgtga cagtatgggg
    13801 gaggcgaaat cctggtgtct gcgggaaatt cccaaacttt ttagcggaaa ataatatttg
    13861 ttattgttaa taaaaagggg cgaaatatcg ccccaattct ataattaatt tgaaccaaac
    13921 ttcggatcgt ttccgtaacg attgctgcca gagttgcctt ctaaacaggc aaatacaaac
    13981 agaactaacc agccaatgat cggaaccaga tagagaagcg cccacgcacc tgaacggtca
    14041 gtatcgtgca gacgacgaac gcacagagca atgaccggaa tgattgttgc tgccaggtaa
    14101 atgagagaaa ggaatggaaa ctctaaacct aaaatcaatt gaatgacatt gataatagcg
    14161 ccgacgatgg cgttaatcag agtaaacatc cagtactctt tacgacgtgc gcgaccagag
    14221 aaaccaacat aattttttaa tacggctaaa taccactgca taaaaacatc cctttaatta
    14281 aaagtgacga aagtctattc cgggtattgt gtaaatgcta ttcgtatctt tctttttcgg
    14341 atgttataaa ggttttgata tttaagataa atatatggtt atttttttat aggtaattta
    14401 tataacgaca ttgaaatatt ttaaataaac attagtaaat tattcattca tcaacataat
    14461 gataaaagcc ggatgagtca gacatccggc gagtagattt gttttaaggt tccagttttg
    14521 gatcaggtcc aaagcggttt tcgcctggcg tacccgcctg gcagttaaag acgatgataa
    14581 taagccagcc gataaacggg attaagaaca gcagtgccca ccacgccgag cggtcggtgt
    14641 cgtgcaggcg gcggaactga actgcccacc acggtaaaaa caccagaata ccgtagattg
    14701 tcgtcaggat gccttcgcca ccggcacgtt gccagcctaa cattttatcc agcagcccca
    14761 gcacgaacgt aaagatgatg ttgaccagaa taaacatcca gtactctttg cgccgcgcac
    14821 gaccccggaa accgacataa ttttttagta ctttcagata ccagtccatt tttgcctcaa
    14881 ttttgctcaa tcacttgttt ttaaagcagc aaattaagaa tagacgtgaa tgaaatggca
    14941 gtaaatattc gttcaaggaa ttaataaagt gccgtaaagc gcgctaagac tgatgacaag
    15001 cgcaaaatcg ccagatgcgc tacgcttatc tggcctacgc ggcattgtaa catattgaat
    15061 ataaacgttt tgtaggttgg agaagacgtt cacgccgcat ccgacataaa taacacgcac
    15121 ttagctaata atcagaggca cctcgtaagg tgcctttttt taaccgaagc gaacatctcg
    15181 tccatgcggt tcatcgagat cctgccacgg accaattgaa ataatccccg tagggttgat
    15241 ggtcttatgg ctgcggaagt aatgattacg gatgtgattg aaattgacag tttcggcgat
    15301 ccccggcatc tggtagatat cgcgcagaaa accgtacaga ttcaggtaat cgctgatgcg
    15361 gtgcttatca cacttgaagt gggtcacata caccggatca aaacggacca gcgtggtcca
    15421 caggcgaata tcggcttcgg ttagctgatt gccggtcagg taacgatgct ggcctaaaat
    15481 ctgctccagt cgcgccagcg attcaaacac tttcgccacc gcctcgtcgt aagcttgctg
    15541 gctggtggca aaaccagctt tatacacgcc gttgttaacg gtgtcataaa tccagccgtt
    15601 aagttcgtca attttcgttt gcagggctgg tgggtagtaa tcacccgctt tcgcgcccag
    15661 cgcatcaaac gcggtattga acatgcggat gatttccgct gattcgttgc tgacgatggt
    15721 gtggttcttt ttgtcccaca gtacgggaac agtaactcgt ccgctgtagt gtggatcggc
    15781 gtggagataa agctgataca aaaattcatg ttgatagagt gtatcgccgg ttgcacccgg
    15841 aaaactgtca tcaaaggtcc agccgttttc cagcatcagc gggttcacta cagaaacgga
    15901 aataaacggt tccagtcctt tgagtttgcg catgatcagc gtgcggtgcg cccacgggca
    15961 ggcgagtgaa acatagagat gataacgatc tttctctgcg ataaaaccgc ctgtgccagt
    16021 ggggcctggc gcgccatcgg cagtgagcca gttacgaaat gcggaagctg aacgttgaaa
    16081 tttaccgccg gtagatttgg tatcgtacca ggtgtcatgc cagacgccgt caatcagttg
    16141 acccatattt ctctcctcca atagcaaaaa cgaggagctt cctcctcgtt ttttatcgtt
    16201 aagtatagcg cgtgcttacc actttttatt cagcaggcgg tcgatgctat acgcgcccgg
    16261 accggtaatt gccagcagca ggaatccgcc agaaattgtc aggtttttca tgaacatcag
    16321 cgagttgacg ccttcagcaa agttgctgtg aaataaaaat gccgtcagca gcgtaaagcc
    16381 cgcagtaaac agggctgtgg tgcgagtcag gaaaccgaac aggattgcca gaccaccacc
    16441 aaactcaagc agaatcacca gcggcagcat aaaacccggg acgcccatcg cttccatata
    16501 ttgttgggta cccgcgtaac cagtaatttt tccccagcct gcggtaataa acagaatcgg
    16561 cattaaaatg cgcgctacca gtacaccaac atcttctaat tttttcatca tactctccac
    16621 tgcgccgctc aggctgctga ttgttttttt gtgcaagggc gcgctattag cgtcgttgct
    16681 gtcgatggag agaatcataa acgtggcgaa gcgtgattgt tagcaaggaa aactgtcaaa
    16741 tattttcaaa aaatttgagg gataaggccg gaatttctcc ggccagagag gaagttaacc
    16801 gcgaagctgt tgttgcttga gggttgtttt caccagacgc caggcgctcc atacaccaaa
    16861 accgcgtctg gcccagcgga ccagcatatt aggatggcga atcgtccaga tagccatcac
    16921 gctactgcca accagcgccc aggagcgcag actcaacagc atattccagc gacgatcgta
    16981 agcgcctgtt gcctccagcc attcacgacg actggcggaa agatccagcc gttgttgctg
    17041 gatctggcta agcagttgcg ccttacgtcg ttcacgttcg actttactgc tcacgggact
    17101 cctcctcgag cagctgccga tcgtttgcca gctcatggcg cgtgtggcgc agcaacgtag
    17161 acttacgcga tttacgtaac gtccagatgc cgccaatcag tgccagtagc agcaacacca
    17221 cggtggtggc aatcatcgca ttcaggcgat attgcggatc aaccgcccag ataattagca
    17281 ccatcaggct cataagacca aatgcagcga aaagcatcgt caggcccagc atcagtaaaa
    17341 gttgaaagag attcgctttt tcctcttcca gctccaccac cgccagccgc agacgtgtct
    17401 ccaccatttc aaccatgatg gaaacaattc gctgcccgat gcccagaacg cttttaccgg
    17461 gcccttgcgc gtgatgagtg tccgccataa ttaacgacgc gacagcagaa caccgagcac
    17521 tacaccgatt gctgcgccaa tgcccacgcc cgtccacgga ttttcacgca catactcatc
    17581 ggcacgcgcc gccgcgacac gggtttgttt ggcaatggca tcaccggttt cacccaggcg
    17641 ataacggctc tgtttcagtg cctgctccgc tttgctacga atcttactca actcttcttt
    17701 cgacttctcg ccagatgagc taagcacctc ttccagcgta tcggaaaggg atttcaactc
    17761 agcacgcaga tgttccgtag tgtgttcttt cgacatagtt ttctccaggt gagtaagtag
    17821 tgactattgt gagttagtag tcgcgcgctt ccagcttttt cagctcttcc tgcgcttctg
    17881 ccagtttccg ttcgcgtttg gcaatcttat ctgcatcgcc tttttgcttc gcctcggcta
    17941 aatcttgctg gcgttccgcc acctcatctt tctgctttgc gattttcttc tgatgatcgg
    18001 cacgcagctg gctatctgaa cagttggccc ggacttcact cagggcttta ttcagaccgt
    18061 caatacgatt ctggttttgg tgtttttcgg catagctgat ctccttaagg atattttgct
    18121 ccttttcctg acacagggta gtggcataac taccggcact aagagcaaag agagaaacag
    18181 ctaaagcgat gcggtatttc attcttgaac cttccattgt gattcggacc tattacaaag
    18241 gaacgattgt ccagacaacg aagcggtcgt gttaaccacc gcctcattaa tcatagacag
    18301 ttacggtgaa atcaccaaag tgagtgatct gtttcggata acaggaagtt atccgaagcg
    18361 atgagagtta tcccgcaacc gggtcagcca ctgcatagcc tgactgttat tgtcctgctg
    18421 cgcaatgatg tagccatggg gcaatgttct gtcgaggacc gcttttgcgg cggcgctctg
    18481 gtcgctggag tcaaaggtaa ttaacagagt gtcgtttttg ggggtgatac ttttgaaagg
    18541 aatgccgtga gcgtcaaggt gatgccagat tgaaaaaccg tctggcatcg ttgtcccttg
    18601 atgaacggca cgaatcgcca gcgtagactc ttgctggcga accgcggacc aggccagcaa
    18661 cagtgtgccg accagaagta aaacagcgcc ggaccaggct agctggcgaa gcgacatgcg
    18721 aggtatttgc atcccttacc cccgatttcc atatttcttt ttccataaca cgaccagaga
    18781 acctgccagg ccaaacacca gcagcaccac cgggagcagc atcaggcatg acatcagctg
    18841 gtcctcgtac tttaaaaata ccggcgtttt gccgagcatg taacccagag ttgtcaggat
    18901 caatacccac aacaaaccac tcatccagtt gaagaactga aagcgcgcgt tattcagccc
    18961 tgataacccg gcaatcgtcg gcagcagtgt tctgacaaac gcaataaagc gaccaattaa
    19021 cagcgccgat aaaccgtgtt tatgaaaaag atggtgtgcg cgttgatgat aatgcgcggg
    19081 taaatgagat agccagtttt gtacggtgcg ggtattgccc agccatcgcc cctgaatata
    19141 gctgacccag cagccgaggc tggcggcaac ggtcagcagc agaatcgttt gcggatagcc
    19201 catcgcgcct ttcgcaatca atacaccgac caacaccagt aagctgtcgc ccggtaaaaa
    19261 ggctgccgga agcaagccgt tttcaaggaa caaaatgaca aacaagacaa aatacaacat
    19321 gccaatcatc gacggattgg ccagggtttc aaaatcctgc gcccacaggg cttgcagcaa
    19381 ttgggtcaaa agttccattc attattcctg gtgaatcggt taacgccacg ctggttttaa
    19441 atgatgcact accgcaatgt tattgctgtc ttggtatgtt ctgcgggttt gatacatcaa
    19501 cacagcctga actattccct gttaaatcgt gagataagaa agcattcaaa agcataaatt
    19561 caaattgaat ctaattgtaa caaaggtcaa cgttgttcgt tatcaaaatt acatgtccgt
    19621 ggagtctgat tttgctgaat gcaggggagg gaggcgagag gatttacaca ggctgacact
    19681 ttatactttt cgcttaccga cgagacaaac aggcgcactg tagcgcctgt cggacaggaa
    19741 tttcatttac tgccgctggt ggctgtatcg agatgcacca ccgggttttc ggcgaacaga
    19801 tagcggtcgg cattgaactc gaaatcgtcg ctggtttcgt taaataacat gatcttggtg
    19861 ttttccaggt gctgccacat tgccagctta gcagcatgag gatctttgcg aatcagcgcc
    19921 ttgaggattt gatcgtggtc atcacaccag ttatcgacgg tacgggcatc aatgtgttcg
    19981 tgcagttttt tccagtacgg gttatgacta cgctgggtcc acattttttc cacgatagcc
    20041 gccagggcgg agttctgcgt cgccagggct acctgaatgt ggaactgcaa atcccactcg
    20101 gaatcacgga agcattgttc gccgcgcgcc tgctcctgga tggccatcag tttcatgatg
    20161 tcctgtttcg tcacctgagt tgccgcgaac tcggcaatat tactttcgat gagctggcga
    20221 gcctggagca actcaaacgg accgtaattg gcgaattcca tattattgtc agtcgcctgc
    20281 tgatggcgcg gctggttgga aaccacatga atacctgaac ctttacgcac ttcgacatag
    20341 ccttcaactt ccagcatgat gatggcttca cgaaccacgg tgcggctgac gttcttttca
    20401 tcggcaataa agcgttcagc aggcagttta tcacccacca gatagacgcc ctgttcgatg
    20461 cgctctttca gatcagcggc aagttgttga tacaaacggc gtggttcagt gatttccata
    20521 tgcgctccag gcatgatacg gcagactata tttgttatac cacttttgcg agtttagctc
    20581 tactcacagt gatgaaaaag ccgcctgaga aggcggcttt gaagggaaac taaaacttac
    20641 acgaagcctt aatgttgcgg tgcgggatcg ttatgtgatt cctgcgccac ctcaatcgcc
    20701 ggtttgttct gcaacacggt ccagataacc agcgcaccta acaggtcgaa cactgccaga
    20761 actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgactac cagtgcaaac
    20821 agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta
    20881 cgaccgaaca cgtcagacga gagcgtaatc agtgcgccag acagtgcctg gtgagcaaaa
    20941 ccaccgatac acagcagcat aatcgcgaca tacgggttgg tgaacagacc gatcataccc
    21001 gggccaatca tcagcaccgc acccagcgtt acgaccatct tacgggaaac gatcaggttc
    21061 acaccaaacc aacgctggaa cagtggtggc aggtaaccac cgaggataca accgaggtca
    21121 gcaaacagca tcggcatcca ggcaaacatc gcgatctctt tcaggttaaa gccgtaaact
    21181 ttaaacatga acagcgggat ccacgcgtta aaagtacccc atgccggttc tgccaggaag
    21241 cgcggcagcg cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc
    21301 ttcgctgtgt caacctggtg ctgggcttcc tgaccattaa taatgtagtc gcgttcttca
    21361 tcggtcagat gtttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag
    21421 ataaagctca acgcaccgga gatgataaat gccatctgcc agctgtgcat cacgattgcc
    21481 cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtaacct
    21541 accgcgatgg aacgctcttt cgccgggaac cattcggagc tggctttcag acccgccgga
    21601 atcatcgcgg cttccgcggc accgaccgca ccacgagcaa cagccaggcc gccccagcta
    21661 cctgccagcg cggttgcacc acagaacacg gcccacagca cagcaaacat tgcataaccg
    21721 attttcgtac ccagcacatc cagtacatag cccgctaccg gttgcatgac cgtataagca
    21781 gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc
    21841 agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc
    21901 gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc
    21961 gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt
    22021 acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc
    22081 taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt
    22141 gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac aactttaaaa
    22201 ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acggcgaggc
    22261 cagtactggc gcggcctgca gcgagattta ccactttgag agtaattttt ttaactacgt
    22321 ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc
    22381 agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt
    22441 tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc
    22501 aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga
    22561 ctatcgtttt aaaaacctgt atgacatctg gttgaagggc gatcactaca aatggcgcgc
    22621 tatgcgtacc aacggtgtgg ccgagcgtct gtgcaccggt gatgcgtctg accgtgaaaa
    22681 atttgacgcc tgggcggcga ctgttccgca cactattggc aacccgttat accactggac
    22741 gcacctcgaa ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc cgtcaactgc
    22801 cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct ctgcgcgcgg
    22861 catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct
    22921 ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg
    22981 gcgtccggac aaagccttta acatcgaaca ggcgaccttt aacgactaca tggcgaagct
    23041 gggtgaagtc tctgataccg acatccgccg ctttgctgat ctgcaaactg ccctgaccaa
    23101 acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccacgcgc tggatgtggt
    23161 gatgtttgct gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggcagg
    23221 cgaaaccctg agcgagcacg aagtggcaca gttcaaaact gcggtgctgg tgttccttgg
    23281 tgccgaatac gcacgtcgcg gctgggtaca gcagtaccac atcggcgcgc tgcgtaataa
    23341 caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaatgaccg
    23401 tccgatggca gaagagctgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc
    23461 gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg
    23521 taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa
    23581 cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtctgttgag
    23641 ccgctttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata
    23701 cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc
    23761 ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta
    23821 cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg
    23881 ctggataacg tcgcggtcgc tttagcagat ttggctgaag gcacagaagt cagtgtcgat
    23941 aaccagactg ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat
    24001 atcgcaaaag gggccaacgt catcaaatat ggcctgccga ttggttatgc attggcggat
    24061 attgcggcgg gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat
    24121 cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcgtgaagtg
    24181 cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta atgagctgtg gatcctgcca
    24241 accgtgggct gtgtcaacgg catcgcgcgg cagatccaaa atcgtttcct gaaagagacc
    24301 aacaacgccg aaggtactga cggcgtgttc ctcttcagcc acacctacgg ctgctcacag
    24361 ctgggcgacg atcacatcaa tacccgcacc atgctgcaaa acatggtgcg ccacccgaac
    24421 gcaggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt
    24481 gaaacgctgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat
    24541 gatgagatcg aagccgggat cgcgcatttg catcagctgt ataacgtgat gcgcaacgat
    24601 aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac
    24661 ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct
    24721 aacggtggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg
    24781 atggaccatt gccgcgacga agcaacgttt gaaaaactgg tcaccatggt caacgacttc
    24841 aagcagtact ttattgccca tgaccagccg atctacgaaa acccatcacc ggggaacaaa
    24901 gcaggcggta tcaccacgct ggaagataaa tcgcttggct gtacccagaa agcgggttcc
    24961 agtgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccggg gctgaacttg
    25021 ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat
    25081 atggtgctgt tcagtactgg tcgtggcacg ccgtatggtg gatttgtgcc gacggtgaaa
    25141 atcgccacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt
    25201 cagctgatcc acggtaaagc gatgccgcag ttgctggaag aattcatcga taccatcgtt
    25261 gagtttgcca acggtaagca aacctgtaac gagcgtaacg acttccgcga actggcgatc
    25321 ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca
    25381 taatgtgaag cgccgtttgc ttttccagac ctgctaagga tttatcatga ccgcgtattg
    25441 gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaaccg
    25501 tgacgaacgc cgctttaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt
    25561 ttttcttctg ggcacctatc ccgccggtgc cagtgccatc cttaatgcca ttcgtacgct
    25621 gattacttta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg
    25681 aattggcctc gcgaagttcc atcatcctgt cgaactactg ccggtaatcg gcacgattgt
    25741 cagtacctgg gcgctgttcc gctgtaaagg gctgaccatg cgctgcgtaa tgtggttttc
    25801 aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca cgatgattga
    25861 ggggagtttt ctgctgatga atggtctgaa tatcattcgt ttctggcgga tgcagaaaag
    25921 gggaattgat ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa ggggttaaac
    25981 aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca
    26041 gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg
    26101 atgataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta
    26161 caggccagtg ggatcagcag cagagacccc cccgccacgc cggatgcgcc acaggcacac
    26221 agagaagcca ctacgctcaa cagcagcgct gtgggcagat cgaccggaat acccagcgta
    26281 ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg
    26341 gctcccagcg gaatagaaac agaataggta tcgcgatcca gattcagctt ttcacacagc
    26401 gccatattca ccggaatgtt cgctgcagag ctgcgggtga agaaggcata cacaccgctt
    26461 tcacgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc
    26521 aatgggttaa ccaccagcgc caccagcagc atacagccaa ccagcacgac cagcagttgc
    26581 gcgtagcccc acagcgtgga gaaaccggtg gttgccaggg tagaagaaac cagaccaaaa
    26641 ataccgatcg gtgcgaagcg aatcaccagt ttcaccataa aggtgacggc attcgacata
    26701 tcgttgatca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaaacc gaggccaatt
    26761 gcccacacca ggatcccgat atagttacct tttagcagcg cgtcaatggg gttggaaacc
    26821 atgctcatta ccagcccgcg cattacttct acaatgcctg acggcggcga aatatcaccc
    26881 gcgctactgg acaagtgcag ggtagaaggg aaggcaaaac tgaagattac tgcggccaga
    26941 gcagcagaga aggtgcccag cagatagagg aacaaaatag ggcggatatt ggttttctgc
    27001 ccgtgctggt ggttagcaat agatgccatt accagcatca acaccaggat gggggcaacg
    27061 gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt
    27121 gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg
    27181 ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt
    27241 cattgtgttg tcgcacatcc ttggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa
    27301 gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt
    27361 ttaaagagag gtcatatttt gcaacttaat ggttacattt atttgacata aacagaggga
    27421 aggactggct actaaggctg attagataac atatgggcaa aactatttgg aatatgagaa
    27481 cggttgcgct cctttaatgt gctatgccat ataaagccac tggcgcaggg agcgcgcagg
    27541 gggcggccaa tcgccgccgc cccctgcacc cccaggctct ggcgaacaaa atcgccgctg
    27601 cgcggttccc tcagcttatc ccttgcggct accgggtcgg gcgcaaggga acgtccctgt
    27661 aagatgcgcc ctcgccgcgc atccatgcgc gtcgccccgg cctccaggga acgcttcggc
    27721 gattttgacg ccaccaacac ccgtgcggtc tttttgtaaa gagctataag ccgattacat
    27781 aattaatagc tcacctttgt ctggaatgag ttatctctag taatggctta atatacagcg
    27841 aatcattctc cccgctgctt atcatgccga taattcaccc aggcgttgat aataaacgtc
    27901 atcaccagaa tgccaaacac cacgcccagc gagacggcga tcggaatatg gtagaagtcg
    27961 acaatcagca tcttgatacc gataaacacc agaatcaccg ccagaccata tttgagcatc
    28021 gagaaacgct ctgccacacc tgccagcagg aaatacatcg cacgcaggcc gaggatcgca
    28081 aacaggtttg aggtcagcac aataaacgga tcggtggtta cggcgaagat agccggaata
    28141 ctatccacgg cgaaaatcac gtcgctcaac tccaccagaa tcagcaccag catcagcggc
    28201 gtggcataca acaaaccatt tttacgcaca aagaaatgct cgttgtcgat ggtgtcggtc
    28261 atgcgcaaat gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc tgattcatct
    28321 tcatgggcga gcgccatctt cacgccggta aacagcagga aggcaccgaa gatatacagg
    28381 atccagtcga actgtgaaat cagccagctg ccagtgaaga tcatgatggt acgcagaaca
    28441 atcgccccga gcacgccata caccagcacg cggcgttgta atgccgccgg aacagagaaa
    28501 tagctgaaca acatcagcca gacaaagacg ttatcgaccg ccagcgattt ctcaatcaga
    28561 taacccgtga gaaaggccag tgcctgtgga tcggcaacgg cgcgaccttc ggtttgcacc
    28621 agataccacc agaaagcggc gttaaacagt aacgacagcg tcacccagac cagcgaccag
    28681 gccgcagcct gtttcatggt catggcatgt gccccacgac gcccctgcaa caacaggtcg
    28741 atagccagca taatggtgac aacaacagcg aatccgcccc atagcaacgg cgtgccgaca
    28801 gtattcatag aagttcctta cacataaaaa aacggccaac gtcggaagac gtcagccgct
    28861 gctttttatg catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac
    28921 gtctgcgtat tcctttcggg ttgcccggtg accggatgtg gtttttcaca catcgtaatg
    28981 acgatcgacc ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc
    29041 agtcagtcaa tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt
    29101 acgctatctg ccggaaaaat caccccagtc tggcggcgga tctcggtcag cagtttagcg
    29161 gtgatgcgac tgaccgccag ccctggatga tccaccagat gctcatccac cagttcagca
    29221 aacagctctg cttcgtagag catggtatta atatgctgcg gttgggtcag atcctgcatc
    29281 tggctgccac gcggcacaaa acacactttc tggcattcag acagtttttc aatcaccagc
    29341 gatcccgctt cgccctgaat ctcgcttgcc aggacagaat cgctcacttt tgagtgctgc
    29401 aacgtaacgc tgaaatcacc gtaatccatc accaccacgc cgtgggcatc cacaccgctt
    29461 gccagcaaac tggcggttgc ctgcacgttt ttcggttcac cgaataatgc caccgccgac
    29521 gccagacagt aaaagccgat atccataatt gaaccgttag agaatgctgg attaaaggtg
    29581 ttgggattct caccatccag gtaacgttga taccgcgagg aatactggca atagttgaaa
    29641 aagactttac gcagtttgcc gactttcggc agcgcctggc gcaacagatg aaagttcggc
    29701 aggtaggccg ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg
    29761 gcggcatcca cttccgccag attcgatgcc agcggcttct cgcaaatcac atgaattttg
    29821 tgactaagga aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc
    29881 gcgtcaatgg catcgctttc cgccatcgct tccagcgacg taaacagatg ctcgacagaa
    29941 aaatcattgg caaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg
    30001 tatttaccgc tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc
    30061 acagcgaaac gtatcataac gcgtggatac tccataaagg gattctgccg tcaatgtagc
    30121 acgccttttt catcttcgac gtgcgcagat acgcattatt tctgcgccgg acagccatgc
    30181 tcactaaacg gcccaatcat gaccgatggc ccgagatatt tcggctggcg atggaggatt
    30241 tttgcgtcaa tcaccgcgcc gacgcgagcg agtttctccc gcagttgagt ataaaaaccg
    30301 tgcttcatat tgaggtctgg cgcgttaaaa ccgatagcgt cgatgccgta ctgttttgcc
    30361 agccagatgg cgcgttggtt atgaaattct tgcgaaataa tggtgatatg gttttcccca
    30421 aacacctttt tggcacgcac caccgaatca agcgttgaga atccggcata gtcgcagaag
    30481 atcactttcg ccggtacgcc tttagcgatc aaagcctgct gcatacctga ggcttcatcg
    30541 taatttttac gcccgttatc gccacttacc agtagccatt tcacttttcc ggcgtggtac
    30601 aacgctgctg cggtatcaat acgtcgggta aagtagcgat tacccggcct cgcccccagc
    30661 aataaaccca cgttacgcgc cggaactgcg ctgacatcgc tccaggtcag ctgcttgctg
    30721 gtgttgacca taacgcgatc ggcaataaaa atggtcgctc ctgctataag caacaataaa
    30781 cagccaattt taagagtccg gcgagaaaaa caaatacgga gaagaaggcg ggcctgcatg
    30841 gtcgttatcc ttaacgaaag gggggaggtc gattttaggc gctataggaa gaaaagataa
    30901 cgcctgacat cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg
    30961 ggtgtcgaac tcaagctggt gatactgcgg ctccatatgg cagcacaatt gatagaacgc
    31021 tttgttatgc tctttctcct tcaggtgcgc caactcgtgc accacgatca tgcgcaaaaa
    31081 cggttccggc gcgttgcgaa acacggtagc gacgcggatc tccgcctttg ctttcagctt
    31141 gccgccctgc acacgcgaaa cggcagtatg taatccgagc gcgtttttca gcacgtggat
    31201 cttattgtca tacatcactt tagtgatcgg cggggcattg cgcagaaact gatttttaag
    31261 atcctgagta tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg
    31321 ttttgccagc acatctccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag
    31381 ctgctcggga taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa
    31441 aaagggtata ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg
    31501 ccgatgagcc acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg
    31561 gatgacgtta acccactaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg
    31621 gacgacacag aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagt
    31681 tgtgcgctgg cggaacataa gccatacagc attggcgact catacatcag cgaactggcg
    31741 acgcgcgaga atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc
    31801 accgccgact acccgcaaca gccgggcgtg gtactgatca aagtaccgaa aacactggcg
    31861 ttgctggaac aacaactgcg tgcgctgcgc aaagtggtca cgccggatac acgtattatt
    31921 gccggtgcca aagcccgtga catccacact tccacgctgg aactgttcga aaaagtgctc
    31981 ggtccgacca ccaccacgct ggcatggaag aaagcgcgcc tgattaactg caccttcaac
    32041 gaaccgccgt tggccgatgc gccgcagacc gttagctgga agctggaagg cactgactgg
    32101 actatccaca accatgcgaa tgttttctcc cgcactgggc tggatattgg cgcgcgcttc
    32161 tttatgcaac atctgccaga gaatctcgaa ggtgagattg tcgatctcgg ttgcggtaat
    32221 ggcgttattg gcctgacatt gctcgataaa aacccgcagg cgaaagtggt gtttgtcgat
    32281 gaatcaccga tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gcccgaggcg
    32341 ttggatcgct gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgtttt
    32401 aatgctgtgc tctgcaaccc gccgtttcat cagcaacatg cgctgaccga taacgtcgcc
    32461 tgggagatgt tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatatcgtt
    32521 gccaaccgtc acctggatta cttccataaa ctgaagaaaa ttttcggcaa ctgcaccacc
    32581 atcgccacga ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcgataa
    32641 gattgggagt tacctgccgg atgcggcgta aacgccttat ccggcctacg ttcagcacat
    32701 tacccccagg tcggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg
    32761 gaatcgcgct tccataaaac aaaaaatccc tgtcaccatc ctctacattc tcttgtttag
    32821 cgtttttcta cgtttatgct acatacgtga agttatgacg cataaagaat acgatttctt
    32881 taccgccgtt catcgtacta aggggaaaaa atgattgcga ttgccgacat cttgcaagca
    32941 ggagaaaagc taactgctgt ggcacctttt ctggcgggta ttcagaacga ggaacaatac
    33001 gcccaggcgc tggaactggt cgatcatctg ctgctcaacg atcctgaaaa tcccttgctg
    33061 gatctggtgt gtgccaaaat aaccgcgtgg gaagaatcag cgcccgaatt tgctgaattt
    33121 aatgccatgg ctcaagccat gcctggcggt atagccgtga ttcgtaccct tatggatcaa
    33181 tatggtttaa ccctttccga tcttccggaa attggcagta aatccatggt gtcacgcgtt
    33241 ttgagcggga agaggaaatt aacgctggaa cacgctaaaa aactggcaac gcgattcggc
    33301 atttctcccg ccttgtttat tgattaacgc gacgtgccgg atgcgtcgca tccggcgctt
    33361 caaacaacga tttaaatctc cagcgccagc cgtgttccct gggcaattgc ccgtcgtgcg
    33421 tcaagctcca tagctacatc gcagccgcca attaaatgca cagttttccc gctatcaatc
    33481 agctgttgcg ccagcgcgcg gtttggctct tgccctgcgc agatcaccac attgtccact
    33541 gctaatacct gcgtttcgcc gttgatcacc acatgcagcc cgtcatcgtc aatcttctga
    33601 taacttacgc ctgggatcat tttcacaccc cgcgagagca gggtggtgcg atggatccag
    33661 ccggtggttt tgcctaaccc ctgtcccggt ttgctggctt tgcgctggag catcacaatc
    33721 tgccgtgggc tacgcgggat ctgcattccc tgcgggctta agccaccagc ctgttgcagg
    33781 ctgctgtcga tcccccactc attacagaac tcggcaatgt tctggctggt ggactcaccc
    33841 ggctgactta aaaacatcgc cgtatcaaaa ccaatcccgc cacaaccgat gattgctacc
    33901 tttttgccca caggcgcttt gtcgcgcagt acgtcgagat aactcaacac cttcggatga
    33961 tcgattccgt cgataggtgg aatgcgcggt acgatcccgc tagcgaggat cgtttcatcg
    34021 aacgactgta actgatccgc cgtcacggtg tgattgagtt tcagcgtcac gcctgttact
    34081 tcgatcatcc ggcggtaata gcgcagcgtt tcgtaaaact cctctttgcc agggatctgt
    34141 ttggcgatat taaactgacc gccgatctca ctgtgagcat caaacaacgt tacctgatga
    34201 ccacgtgccg ccgcgttaat ggcaaacgcc agcccagccg gaccagcgcc gaccaccgcc
    34261 agattttttt tctgcacggc gggaaggatc ggcattttgg tttcgtggca ggcgcgagga
    34321 ttcaccaggc acgaggtgac tttgccaacg aagatctgat cgaggcaggc ttgattgcag
    34381 ccaatacagg tgttgatctc atcggctcgc cccgattgcg cttttgacag cagttccgca
    34441 tcagcaagaa acggtcgcgc catcgatacc atatcggcat caccgcgcga gagaatgtcg
    34501 tcggcaacct gcggatcgtt aatccggttg gtggtcacca gcggcagcga gacgtggctt
    34561 ttcagtttgc gcgtgaccca gctaaaggca ccgcgcggca cgggcgtggc aatggtcgga
    34621 atacgtgctt catgccagcc aatgccggtg ttgataatgg tcgcgcccgc cgcttcaatg
    34681 gcctgcgcca gccctaccgt ttctgcaaaa gtaccgccgc cttctaccag gtcgagcatc
    34741 gacagacggt agataataat gaagtcattg ccgacgcgtt cgcgcactgc tcgcaccact
    34801 tcaactgcaa agcgcatccg gttgcggtaa tcgccgcccc actggtcgct acgctggttg
    34861 gtgcgcaggg tcagaaactc gttgatcaaa tacccttcgg aacccatcac ttctacgccg
    34921 tcgtatccgg cctcccgcgc cagttgcgcg cagcgggcga aattgtcgat cagttgcagg
    34981 atctcttcat ggcttaactc atggggcacg aaacggttga tgggggcctg caatgcggag
    35041 ggagcgacca gatgcggttg gtagctgtag cgcccggtat gcaaaatttg taaggcaatt
    35101 ttgccgcctt cctgatgtac ggcttcggta atggtgcgat ggtgtgggat ctggctggcg
    35161 tcgttgagca ttgcgccgcc ttccatgcca acgcctgtta aatctggtgc aatgccgccg
    35221 ctgacaatca acgccacgcc atgacgggcg cgttccgcgt aaaacgctgc cagccgttca
    35281 gcgccgtccg ggtattcctc cagtccggtg tgcattgaac ccatcagtac tcggtttttt
    35341 aacgtggtga agcctaaatc cagcggggcg aacagcgacg ggtagctcat aaaattgtcc
    35401 agtatgtaaa ataattgtta tgtggtcgga tgagttctaa tttagccgtc gctggagaaa
    35461 agggaaaagg ggagtgcggg gtttgtgatg ggattcaaaa agaatacgcc cgttcgtcat
    35521 gccggacggc ggtaatacag tcgtccggca tcgggaaaaa ttactgctta cggaaaaaat
    35581 cgttatacaa catatacaaa tgcgcggcac tccaggagaa atttggtgcg ccttgctgtg
    35641 cgccagtcag cgggttgtaa ttctcctgaa ttgggccatc ggcggttaat cctttggcgt
    35701 gccggaagaa cgtatccgcc agcttcaggg catcatcgcg ataaccgtaa cgctccatcc
    35761 ctttcagacc aaaccagaac tgatccaccc acacgcgccc gcgccagtaa atatctgcgc
    35821 caaaggccgg attggttaac gccgccgttc ccagcggtac aaaggtgttg aactctttag
    35881 gatcgagcat caccttcacc accgcgtcgg cgttggcctg cgttgccgca ccgttaaaca
    35941 gcggcgacca gccttccggc cctttgccgc gctcgacaat cggtttgccc gcgcagccgt
    36001 tcgccagtgg tttatcttca atgcgcacgt catagtagaa ctgggtagtc gggtcgaaca
    36061 tacaggtgtt gatgtagtcc gcgagctgtt gtgctaactg acgatagcgt ttggcctctt
    36121 ccggcttacc gagaatcgtc gccatctccg ccagataatg gttatcgctg tacatatagc
    36181 tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg tcctgactgc
    36241 ggttttcggc gaatttcacc gtccagtcgc tacgtttgcc gccgttagcg acatatttat
    36301 ccagctgttc tttgtcgata aacccaaaga cggcggcatc atcacgcccc gattcccacg
    36361 acgccgcaac ctgtgccggg atttccagac tgtcgtactg gcctttctct accacgcggg
    36421 cgtagttgtt cagcccagac agcgtctctg ctttgttgcc tttcttcacc gtaaacagca
    36481 tctcgccgct ctcagtgttg tgggctttgt cgcgggtcgc gccatattcc ggcacgccgt
    36541 tgccgttgtg atcgcggtta cgtagccacc agtcgtgata ggccaccagt ttcgggtaca
    36601 tctctgccag ccaggtttta tcctgggtga cgttatacac ttccattact gaccaggcgg
    36661 caaggctggg tttggtatta cgtttgttcc agttgccgcc atcgccgcca cgctcggggc
    36721 taaggttcca tgctatcagg tcggggacaa agcccacatc ctgcggacgc acgcggtcgc
    36781 ctggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgata tccggattga
    36841 aatgcgccat cgcaaatgcc tgcttccagg tatcccacgg ccaggtctga ttgccggaga
    36901 accagcgccc ggtcaccgac ggcgtgacgg tgttatattt caccgccccc ccaggcgagc
    36961 gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg
    37021 catccggatt ggttaaccct ttcttcagat actcttccca gcgttgctgc gaggcggtga
    37081 gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg ctaacttcct
    37141 gagcggtcag caggtgggag taggtggtat agagcgtggc cgaaccgttg atatgcgcct
    37201 tactggtaaa gcgattaccg ttgatttcag tctgcactgg cagggattta tgcacctgat
    37261 attctgattc accggaggtc agcagatccc aggtggcgcg cactttgcca aaggtgactt
    37321 tcaggccatc acgggtggcg ctgattttgc gctgatagtc agggtattcg ccagcaatgg
    37381 ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagc tcgccgtccc
    37441 acaccagatc cagcggttta ttgctgatga ttttagtttc cagtagtgac gtgcgcggcg
    37501 tggcgaagcg cagagtcatt tcgacctgca catcttttgc tgtcagtttt tgcaccagcg
    37561 cgccgggaat actgtatgcc tccagcgtga agtcgacttt cttgccgtct tgccagacgg
    37621 tcagacgatc gaaattgctg gccataaagt tgatgtattc ttccgtcagc agcgcaacgc
    37681 ctggaaaacc gcccatggtg ttcgggccgt ctggcaacag atgaccatgc caggctccga
    37741 gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg
    37801 gcgcgccagt acggttaatc acgtttttat aattgtcggc gttagcggca tgggcggaaa
    37861 aacttatcag cagagcgcag gttactggcg ttaaaatagt tttgattttc atgaaattac
    37921 agtccttatt accagtagaa atattgcatc aggaagagtt ggtcgctggg atcgtgagag
    37981 gcatttttca tgtagaagcg gctatcaaaa gccgttgcga aacgtccgcc gccgtattcg
    38041 taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt
    38101 ttggcgtagg tgtaagcggt ggagagataa atgcctttta acgcttcata catggcgctc
    38161 accatcatcc cgctttcact tcccgatacg tccagcccgt cggcgcgtcc ggtatggtgc
    38221 caggcgcgag cggcaaagtt aggggtaagc aggcccgtca gatagagttg gcctgtgccg
    38281 tcggtgattt ccagaccgtt catccaggtg atgccttcgg caaggtcata ctggatgtaa
    38341 ccgttcatca ttgccggata ggtgtatttg tcgtcgtagc gatcgtactt gccaaaatgt
    38401 agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg cggtaacgct ataacgaaag
    38461 ttgccgctta agttctgcac cttcagcgcg gtcataatgt cgcgggtatt gggaatgacg
    38521 tagcccagat cgggcgtgaa atcgccccac cattgcaggt catccagcga ggaatcctga
    38581 cgcagactga gcatgatttc ggtgccatca ttggtacgat aaccgccata gaaacggtta
    38641 atcccacctt cgaaaccgcc ccagctatgg tcaggcaccc aggcattacc gtcgtggtcg
    38701 gcctgcacgg tccagccttc gccgtaaatc aggccgaacc aggatccgta cttcatctcc
    38761 aggccacctt caatataggt gccgtcgctg tatttatgtt tattctcacc ctctaaatcc
    38821 atatacccac caacccctaa ttcgccgtaa aaacgaaatg aaggagaaga tgtcgaggtc
    38881 attgcggggg tttttgtgtc gtcatgttct gccggacgtg cttcatcagc ggtcacagaa
    38941 aaggcgtagc aaaacggcag cgcaagtaat gctctgcatg gtgcagtgat taatttcatt
    39001 gtgttaatcc atgagctaaa gaaaaagtac agccttttat cgttgtaatt ataaaaatat
    39061 aaaggcagaa actattttcc ctctccccat ccaggaagag ggaaaatgcc tgctcatttt
    39121 ttattattct gcttaatcaa cattgctggc tggcgtcgct tcaatatggc gctcttcagc
    39181 cgttaatccc tttatatatt tactgtattt ccaccacgca aagccgagga agataacaat
    39241 accgccgacg ttataaaaaa tgatggtcag aatattcgcg ccagtcggga atgtcgaagc
    39301 gacaaaccct acggcaaata tcgcaatcag cattgaaaca acgataatac cggtgcggcg
    39361 ggagcccata cggaaatcgc gtggcaggtg atctaatttg gcgcgtaaat tcaggtaagc
    39421 cagcatgatg aataacggcg gaagcatgga cgctgcggcg gtcatattaa taatggtatt
    39481 catcagatct tgcacggtat tggaaccgag catcgggata atcatcagcg ggatgacgat
    39541 caggaactgg atccacgctg cgcgcgccgg aacgccgttt tcgttcagtt cgacggtttt
    39601 cttaccaaag atgccttccg ggatttcgga gaagaaaatt ttcaccggcg ttgcggtcca
    39661 catcagcagg gaaccgaaca tcgcggtaaa ggacaccaga ccgacaaagc gattcatcaa
    39721 cgcttccggc agaccaaaat acgccgccat gccgtggaat acctgcaccg atccgccagt
    39781 aaatttcaac tctttgctgc tgacgaagac gttaatcagc accgaggaaa cggaatacag
    39841 tacgccgata aaaatcccgg cgaggatgat cactttaacg aacgatttcg aaccgccttt
    39901 gacgtcgtta acgtacaccg cgacggactc cgcgccacct gcggccataa agatccaggt
    39961 ggtaacgccg aggaacgccc agttgaagtt cgggatcatc gcgtcaacgg tgatcggatc
    40021 ggcaggctgt acgccgccaa ccagtgccgt acctgccagt aaaatgtagg agagcgttaa
    40081 cagcagcatc agcgttgaag tgacggaggt aattggcccc agcattttcg ccccgttggt
    40141 ggaaacccag gtggagaagg cgaacagcac catactgata atggtggtgg caaccggcgt
    40201 cataatatat tcgtagccga ggaaggcata cgaagcataa gcaataacgc gcggcaacag
    40261 tgaggtgaaa aagaacaggt ttacgaacca gtaggtatag gcagtaataa atgcccaacg
    40321 cccgcccagc gaacttttta cccacgcgta gacaccggct tctgagtttt tatttaacga
    40381 aacaaattct gcgatgatca gacagaaggg aataaaataa aaaatcgtcg cgaggaaaaa
    40441 catcggtgcc gaggccagtc caagctcaat attattattg ataacgttgt taaagctgta
    40501 aacggcggca aaagtcagcg agagcaagcc gaatttgccg attgtattac gtttggtatc
    40561 agacataaca catctccgaa aatgagtagg gtagcggtgg cggcaaactc cttttgccgc
    40621 cgtagttgtt atttgttatg gaaataacca tcttcgatgg tgactttgag aaccactttt
    40681 ttgaccgcgt tattgcagat aaaccgatat gcttcatgga tatcgtaaat aacgatttgc
    40741 ccttcgtgga cctctacggt ttctccgcag ccttttaaat attcacggtc agtttcatca
    40801 cgataatatt ccacgacctg taatgtctct ttcggcgcat attcaatttt ttgctgccct
    40861 tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg taaatagcgc atcggtcgcg
    40921 gagtccgtct ccacgcggta agtcaatgag tcaccgatgg agtgggcgac gccaggctga
    40981 atgttgtcga tattttcaat cgcttcaacg cagcgttgcc acttcttgcc agaggcgtaa
    41041 atctggcgga actgttctaa gttatcgatg atcctcattg cttattctcg ctgtgcaaat
    41101 tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag gctttgcgcg gtagcttctc
    41161 cgccagaaac cggcagcaac gtaaagccgt agctgaagtc acggaaccag acgcgccagg
    41221 agtccagcac ctcgctgccc caggagttgg agccgaggcc aagcagctgg tggtcgagat
    41281 tcagggtgat gtcatcactg cgctgcaact cgttacagtg ctgggcagcg tggatgtttt
    41341 cctgggtata gcgccaggcg ctgaagttaa tggggcgctg cggaacaacc agcagaccat
    41401 tgccgtggcg gttagtcagt gccgtccagc ggacatgctg acggttgccg ttgttctgtg
    41461 ggaagggata gttctcgaac atggcatcga cggtgctgcg ccagatatcg atgatgttag
    41521 cctgctggct gtcggcgtag ttttcgcccg gtccacgacc gtaatacgcc acctggtcgt
    41581 attcgccgtt aatccccatg gtgaaaccga tgcacggaat gatgtgcgga tagtcgccgt
    41641 aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc agcgatgcgc cagatgtagg
    41701 tgcagcgcat cccgaagtca aacaccggtg gggcaataac cgtgcggctg atgatcaaca
    41761 cttcaccatc gctctgttct acggcaaagt cgcgcagatg ttcctgcatg atctgcaaat
    41821 gattcggctg ccacagccct tcgtactcct gcttgtggtt gtcgatcatt ggcttgaaga
    41881 agttgatttt tggctcgcgg gttagcagcg actcgccatt cacctgccag gatgtcggtt
    41941 taccactcgt ttttgagaag gtgatcgcaa agttgtagcc gcgaacagta cagctcaaac
    42001 gatcgtcttc cagcgtcagc ggacgggcgt tattcggtgc gaaaggtaca ggctgcgcgg
    42061 tgttttcctt cagcgggaac tgataagtgg cgatagaatg cccggcttcg ctgtagcggg
    42121 tgcgggaatc tttggtcacc gtaatgttga gaaacgcttc gcgggcgtcc agctgtggca
    42181 gcgtgatctg taagggggct tcgctgttcg gcgcaacgtc gcgcagttta atctgttgcg
    42241 tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag ggtgtagtca tcaagcgtgg
    42301 taaaccacag attattttcg actttcaact cgccgcgagt cagatccagc gcgtggattt
    42361 ttactggcgc gataacctgt ttgtactctt tcaggcccgg tcccggcgtc tgatcggaat
    42421 agatcaaacc atcaagacag aagttatagt tgttgggata gtcgccgtag tcgccgccga
    42481 atttatacca gacattgccg ttgtcatcct gcgcctggat cccgtggtcg caccattccc
    42541 agacataatg tccctgaatg caatcatgct tatagaagac gttctggtac tccgtcagcc
    42601 cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc ttcggatgcg
    42661 ggtattcacc aaactcattc atcagcggca cgcgggtgta catggtggaa ataatatcga
    42721 ccacttcagc atcgcgatct tcttcgtaat gcaccagtcg cgtgtcatcc agcgccttcg
    42781 ccgcatggta catcgcgcgg atgttacagc catagccgga ttcattgccc agcgaccaga
    42841 tgatgatcga cggatggttt ttctgcgcgt gaatatggcg aacaatgcgc tcgacgtaaa
    42901 ctttttccca ctgcggatcg tcggtaatac ggctgatatc gccgacatta gcaaagccgt
    42961 gcgattcgac gtcggtttcc gccatcacaa acaggccgta gatatcacac agttcgtaaa
    43021 aacgtggatc gttcgggtag tgagcggtac gcacggagtt gatgttgtgc tgcttcatca
    43081 actggagatc tttctcgacg cgatccatcc caacggcgcg gcctttgcga tgatcgttgt
    43141 cgtggcggtt gacgccgtgc agcatcacat aacggttatt gatccagaac agaccgtcgc
    43201 gcactttgat atcacggaag ccaactcgtt gtggcaccac ttccagaacg ttgccgtcgg
    43261 cgtctttcag cgtcatgacc agatggtaaa gataagggga ttctgctgac cattgctgcg
    43321 gctgttcgac agtaaaagca aaactggcgc tggtcagttt ttcaattgcc aaatgatcaa
    43381 tggtgctgct gtgcaccacg tgttcgccat caaacagggt atattccagc gtcgtgacga
    43441 caggggaggc ggcgagattt tccagcacca cttcgcagga aagtgtggca tcgcaatagg
    43501 cttcgtcaaa gtcggtacga acggtgaaat cgttaatatg cgtgagttgt tttccgatca
    43561 gataaacatc gcggaaaatc cccgccgacc accacatatc ctggtcttcc acgtaggtag
    43621 agtccgccca ctgcatcacg cgcacacaca acaggttgtc gccggtttta accatcgcgc
    43681 tgatgtcaaa ctctgcggtc aggcgactgc ctttgctgaa ccccacatac tgaccgttaa
    43741 cgtagacttc aaaataggtt tcgacaccgt caaatttgat cagcgtctgt ttaccctgcc
    43801 agccgtcgct gagggtgaaa atgcgttgat aggcaccggt tgggttatcg ctggggacaa
    43861 acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg ccgtgacctt
    43921 ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac tcagaggtga
    43981 aggcttctgg cacttgcagc ggatggtcaa aaaagtggaa attccactga ccgcttaagg
    44041 gcagaaacag gctgctggtt tcacgggcaa aagtacgagc ctgcgcaaca gaatcatatg
    44101 aaaaaaagta cgcacgcggc gcaagtcggt tttcgtgggt gagctgaatg ttttcccagc
    44161 gattcatagg gcctcccggt agagagaaat cggaaccatt tatggtgatg gtagtttgtg
    44221 taattgctgg ggagtttagt aaaaatttta ctaaaaagta gcggataaat gcatttactt
    44281 taactggatc acgaaaataa acggcagcac tgtccggcaa aaacgattgc cggacagcgt
    44341 gcaaggggat ttaacgggtc gtgccgcgca gttttaattt gctgggaacg aagaccaaca
    44401 gcggcagcgc gcgaccatcg cgggcttttt catacaccag gttaacgccc tgacttccca
    44461 tcatctcgga atggatgcgc acggtggaga gcggcggaaa ggtaaatcgc gcggtgggga
    44521 tatcgttaac gctgataagc gaaatatcct gtgggatgtt caggccacgt tcatgaattg
    44581 cccgcagtac gccgatagca atggaatcgg aagcaacaaa cagtgccttc ggatagtctt
    44641 cccgcgccag catctgtttt gccagttcat agcccgacga actggaaaaa ccgccgcgcc
    44701 agatatcctc ttcgcgtacc acgtgtttca gacgaccata ttccgcaaag gcgacctcac
    44761 gaatatccgc cttgccaggc aagtcttcac cgccaataaa accaatacga ttaacgccct
    44821 gattgatata gaaatcgatg atttctttac tgatacgtgc cagatcgata tccaccgcat
    44881 cgtaaccgct gccgggttcg tgaaagtcga taaaacagat attgtcggtc aacgcacagg
    44941 cggcggcgcg cagggcggac gtgggtttgc cgacaattaa aataccggtg acgtttttaa
    45001 tgtctggtaa gccgttgtgt tcataacagt tggtcagttc gatcgccagc ttttcgcact
    45061 gagtttcaat gccgtggcgg atcgccagat agtaaggatc gttgatctcc agctcctgtt
    45121 ggtagctgta gatagccaga atatggtgtt ggttgactgc gcctgtctgg agtttacggg
    45181 cgctactggt cttgtactcc agcttttcgg cgatctcgag aatgcgatgt ttcgtctctt
    45241 ctttcacatt caatgtcgga tcgtcattca agaccctgga tactgtcgcc agggatacgc
    45301 cagcttcgat tgcgatgtct tttagtgttg ccatgtttac cttttttact ttccctgatg
    45361 tgcgccccca ttgtagagga agtgcttatc gggtcacctg ttttcagtaa ataaagcgcg
    45421 tttctttgac gctgtaacgg taacacagcg gcttgcgaat gtgatcacgc ctgcattctc
    45481 catttttagc ctacaggatt gagaaaaact tttgctatag tttagtaaaa ttttactcga
    45541 agggaggaaa caatggaaac cgtggcttac gctgattttg cacgtctgga aatgcgcgtc
    45601 ggaaagattg tggaagtgaa acgccatgaa aacgccgaca agctgtacat cgtacaggtt
    45661 gatgtggggg aaaaaacgct gcaaaccgtg accagcctgg tgccgtacta cagcgaagaa
    45721 gagctgatgg gaaaaacagt ggtggtctta tgcaatttgc aaaaagcgaa aatgcgcggt
    45781 gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgctg
    45841 ttaacgccgg agcggatgat gcctgcgggc gtacgtatcg tataaaaaga tcggatggcg
    45901 acgtcgtatc gccatccgat ttgatactac gcttcttcga cactgactcg catggccgcc
    45961 agcgccttac gcgccgcttt gatcacctgt tcacactgtt caatggtcag tgtcagtggc
    46021 ggttcaatgc ggatcgtttt agcgttgttg agtgttccgg ccaccaggac gcgctggcgg
    46081 aacatctcgc tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac
    46141 atccctttac cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacgaaaa
    46201 ccgtccagca acatatcgcc tttttgttca gcctgagccg gtaagttctg ctccagcaac
    46261 acattgatgg tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta
    46321 tgcaggaacg ggttgtcgaa cagaacggaa aacacctctt cagtggcgat ggttgcgcca
    46381 atcggcatca cgccgccgcc gagggctttt gccagacaca ggatgtccgg ctgtacgttc
    46441 tcatgctcgc aggcgaacat cttgcccgtg cgccccatac cggtttgcac ttcatcgagg
    46501 atcatcagtg cgccgaactc atcgcacagc ttacgcacgg cggtgagata tcccggcggc
    46561 ggcagaatga cgccaccttc gccctgaatc ggttcgagga tcaccgcagc aacatcatca
    46621 ccggtttttt tgcactcgtt aagagccgtg cgcatggctt cgatattgcc aaacggtaca
    46681 tgacgaaacc ctggcagcag tggcataaaa ggtttgcgga aggtcgattt cgccgtggcc
    46741 gacagcgcgc caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaacttg
    46801 ccgcgcggtg actgataagc cttcgccagc ttcagcgccg cttcaacgga ttcggtgccg
    46861 ctattacaga agaagctgta tttcagttta ccgggcgtta gcgccgcaag ggttttcgcc
    46921 agcatcgccc gtaacggatc gagcagctcc tggctgtgaa gcggttgttt agcaagttga
    46981 ttctgtacgg cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct
    47041 cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa gcgtatttaa accccccgct
    47101 tgccactcta cggctccgta atccccgcca gcggtaacag atttgcgata ctctaaaaac
    47161 cccggattga catgctcttt gaagtattca atcacctccc ggttaagtgc tttcatctcc
    47221 tcatgatcca gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc
    47281 gatgcgctcg aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac
    47341 cgatttaagt attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac
    47401 acaaatgtgg ttatcatttg cgcaatattt aatgctattt gttaacatct gttgttattt
    47461 tgcacggttt ttgtcgggta tggatttggc gattgctgcg caaatgcaca ctttcagggc
    47521 aacaactgcc tgatttttgg gcaagataat taacgttttc gtatagtaac agagtgaaag
    47581 tattttaaat caagggatta gatttatgct ttaatcgcaa attgcgatct aaatcaaatt
    47641 aatcagttaa agataaccgc agcggggccg acataaactc tgacaagaag ttaacaacca
    47701 tataacctgc acaggacgcg aacatgtctt ctcatccgta tgtcacccag caaaataccc
    47761 cgctggcgga cgataccact ctgatgtcca ctaccgatct gcaaagctat atcactcatg
    47821 ctaatgacac ttttgtgcag gtgagcggct ataccttgca agagttacaa ggtcaaccgc
    47881 acaacatggt gcgtcacccg gatatgccaa aagcggcgtt tgcggatatg tggttcaccc
    47941 tgaaaaaagg ggagccctgg agcggcatcg tgaaaaatcg ccgcaaaaat ggcgaccatt
    48001 attgggtgcg ggccaatgcg gtaccgatgg tgcgcgaggg aaaaatcagt ggctatatgt
    48061 cgattcgtac ccgggcgacg gatgaagaga tcgcggcggt ggagccgctg tacaaagcgt
    48121 tgaacgccgg acgtaccagt aagcgtattc ataaaggcct ggtggtgcgt aaaggatggc
    48181 tgggtaaact gccttcatta ccgcttcgct ggcgggcgcg tggcgtgatg accctgatgt
    48241 ttatcttgct gacggccatg ctatggtttg ttgctgcccc ggtggtgacg tatatcctct
    48301 gtgcgttagt ggtattattg gcaagcgctt gttttgaatg gcagattgtc cgcccgatag
    48361 aaaatgtcgc ccgtcaggca ctgaaggtgg cgaccggaga gcgtaatagt gttgagcatc
    48421 tgaatcgcag cgatgagctg gggctgacat tacgcgcggt agggcagctt ggcctgatgt
    48481 gccgttggct aattaacgat gtctcaagcc aggtgtccag tgtcagaaac ggcagtgaga
    48541 cgctggcgaa aggcaccgat gaactgaacg aacataccca gcagacagtt gataacgttc
    48601 agcaaacggt ggcgaccatg aaccaaatgg cggcgtcggt gaaacagaac tctgccacgg
    48661 cgtcggctgc cgataaactt tctatcactg ccagtaatgc ggcagtgcag ggtggggagg
    48721 cgatgactac ggtgatcaag acaatggacg atattgccga cagtacccag cgcattggca
    48781 ccattacttc gctgattaac gatattgcgt ttcagaccaa tattctggcc ctgaatgcgg
    48841 cggtggaagc ggcgcgtgcc ggcgaacagg gcaaaggttt tgcagtggtg gcgggggaag
    48901 tgcgtcattt agctagccgc agcgccaatg ctgccaacga tattcgcaag ctgattgatg
    48961 ccagtgctga taaggtgcaa tccggttcgc agcaggtaca cgccgccgga cggacgatgg
    49021 aagatattgt ggcacaggta aaaaacgtca cccagttgat agctcagatt agccattcaa
    49081 cgctggaaca ggccgatggg ctttccagcc tgacccgtgc agtggatgag cttaacctga
    49141 tcacccagaa aaacgccgag ttagtggaag agagtgcgca ggtgtcggcg atggtgaaac
    49201 accgcgccag ccgactggaa gacgcggtga cggtgctgca ttaatcgata tgccgcatcc
    49261 ggcggttggc gcacaatgcc tgatgcgatg ctggcgcatc ttatcaggcc tacgggggta
    49321 gggcacgcca catccggcaa tcggtgatta atccagttgc gtaatatcaa aagcggcgcg
    49381 gtcgatcacc gcaatgatct tttttatttg tgcatcagtg atatcgctct ggttgacgcg
    49441 taaatccagt accgctttaa aattatccag cgcccgcttc atttgcgggt tctggcgcag
    49501 cgcagcgcca acgcaacgcg ctttgatgcg ttcttcaatc atctctacct gttcgcggtt
    49561 ttcttccagc cactgcgcgc cttgttcggt cagcgcaatc tgcttcttac caccttcctc
    49621 ttcgcgaatg gtaatcagcg actgctcctg cagaaaatcc agcgttgggt agatgacgcc
    49681 cgggcttggg gtgtaattcc cctgggtcag attttcaatc gctttaatca actcgtaacc
    49741 gtggctgtca tcgcgcgaga gaatatccag aatcaccaga cgcaattcac cgtgaccaaa
    49801 gaaacgttgt cgacgaccgc cgccgcgacc gtggcgacca ccgcagcatt gaccgtgttc
    49861 atgctggtga ccgtgtccgc agtgctcgtg ttctgacttc tcacctttgc agcagccctc
    49921 atggcgtggc tggccttcat gtttacagca cccttcgtga tgatggctca tatcagcctc
    49981 cttttgttta attagatata tctaatttat atctgatatg agcattactg caagtacaat
    50041 aatgattttt aaacgtggac tcatctcaca cgtaggccgg ataaggcgtt cacgccgcat
    50101 ccggcgttga gtaatgatgc ctgagaagag gcggttcgtc ttatcagact atagacatat
    50161 cagttctaca aatcgcttgc atttatcatg attaacaatc attatcattt gcgaaatttt
    50221 atttagatat atctgattac catcatgaag gcgataacaa tgaataacac cccccgctac
    50281 ccacagcgcg ttcgcaatga tctgcgcttc cgtgaattga ctgtgctacg cgttgagcgt
    50341 attagcgtcg gttttcagcg cattgtcctt ggcggcgagg cgctggacgg ttttacatcg
    50401 cgcggctttg acgatcacag caaactcttc tttcctcaat ctgatgctca ctttgtgccg
    50461 ccaacggtaa cggaagaggg catcgtctgg ccggaaggac cacgcccacc gtcgcgtgac
    50521 tatacgccgc tgtatgacga actacgccat gaactggcga ttgatttctt tattcacgaa
    50581 ggtggcgtcg ccagcagctg ggcaatgcag gcgcaaccgg gcgataaact cacggtggca
    50641 ggtccgcgcg gttcgctggt ggtgccagag gattacgcgt atcagctgta tgtctgcgat
    50701 gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa
    50761 ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac
    50821 cttgatggtt ttaatatcga atggctggcg catgatgagc aggcggtaga tgcgcgtctg
    50881 gcgcagatgc aaatccctgc cgatgattac ttcatctgga taaccggcga aggcaaagtc
    50941 gttaagaatt taagccgccg ctttgaagcg gaacagtatg acccacagcg ggtacgtgca
    51001 gcggcttact ggcacgcaaa gtaactgata tataaaaaga aaaaggctga cgatttctcg
    51061 tcagcctttg ctatatctgg tggcccctgc tggacttgaa ccagcgacca agcgattatg
    51121 agtcatgagg caagtccatt ctgtattgtt ttggtttgtt cgtaattgtt ttattaattg
    51181 tttataatca actaattatg taatttcttc tgtttccgct tggttccgtt ttttctccat
    51241 tgtacgatcg ttacctgacc cattacctga cccaaattta cgagttattt tgattgttac
    51301 tgatttagaa aagaggttct atggccgtca atctaactga aacagctatt cgagcattaa
    51361 aagcaaaaaa gacatcgttc tatgtctgga gcaacagctc tcaacgtggc actggacgac
    51421 ttggcgtcaa agttcagtct tctggcagca aaattttcta tttccgctat tacgtggaaa
    51481 aagggacaaa agaaagattc attcaattgg gtatatggcc tgagatgaaa ctggccacag
    51541 cgaacgaact ggcaaaaaag tatggtgcct ggctcattga aggaaaggat ccccagacag
    51601 aactggagcg acaacagctt gcagaacagc agcggagaca actccatcaa tcacagggtt
    51661 catttgaagc attgattcat ggttatgtta acaaaatgaa aattgataat aagagaacat
    51721 ggcaggatgt tctgaaacgc cttgaaaatg aatgctatac agtgatcccc cgtgagacta
    51781 aagctaaaga tgtgacttcc ggacagatta aacatatctt ggccgggatc attcagcgcg
    51841 gtgctgtggt tcactctaac cggatccgtt cttacctgat ggctgcattt aactatggtc
    51901 tgaaagctga taacgatccg atgaacacaa gtgtagggat tagttttggg ctggaggcca
    51961 atcctgtatc agtgataccg aaacagtcat ctgcagaaaa agtcggtgac acctggctga
    52021 cactcgaaga gttgcgtttt ctaatggaac attttgctga agcaaccaac gttggattat
    52081 tgatgcaaca tttgattcga ttatgtatct ataccggagg gcaacgtccc tttgaaatga
    52141 tcgccagtca gtggtgtgat gttgattttc agcaaaaaac gctgctggtg actgcggatg
    52201 tctctaaaaa taaacgagag catttgatac cgttgactga atcagcatta caggaattat
    52261 cctgtgtgca ggagttaaca aaagaaaaat cgagccctta cattttcccg ctttcgacta
    52321 atggtaagcg tccggttcgt acggacagtc tggctcgcgc tatcatgtat ttccgggctt
    52381 gtaatcctga ctttaaaatt tttacagcac gtgacttacg tcggacctgt aaaacgttaa
    52441 tgggagaagc tggaatcagt aaggaaatcc gcgatcgtat tcaaaatcat gcgttgaacg
    52501 atgtcagttc aaaacattat gatcgatacg attatttgcc agaaaaacgc agagcacttg
    52561 agatctggga ggatcgggtt aataactatc aacagcaaac aggcaataat gttgtcaatc
    52621 tgtttggcag aaggtgaggg agttcctggt tactgtacga attgcgtaca attgatgggt
    52681 attgttgatg cacctagttc ttgtattgtt tactataatt attgaggtaa attttcgtgc
    52741 ctgtattact cagaggggat tcaaaaatgg ctgtaattcc aatgtcttac tcccctgcta
    52801 ctgtagctcg tcgtttttcg atactggatg gagtaactat tcagggcgtg ctttaccaga
    52861 tcatctggga tccaaagact ccgttcgctg cggtaatcga agctgcacct tctgttattg
    52921 atggtgatat tcgccataaa gttgttgcca ctctggagct tcagcgtcgc tcacagcttg
    52981 aaggggtgtt tgtccggaaa ttctgggaag agcaggacgt cgctcagatt gaagggattg
    53041 ttgttgatgg ggctgtacgt gatgttggcc ttgcgacgtt tgtatatgaa acggtagcca
    53101 caaaagcggg cgttatacta ttaagtgata acgagcaata tgaaggaggt aaagccctct
    53161 ggcagcatat agcgcgtcgt tcaacgaatc tgaaagtgtt tattttagat actgattccg
    53221 cacggtatta tccgtttgat ggcgatcgga tctgctatga cggggaaagt attcctgaat
    53281 cagaaatctg gagtgaacat cctgatcgaa ataaacacgg tgttgttctt gtggccgaat
    53341 ctgttaatgg aaaagccgca taatttgcca gccgcatcgt attcttggaa ctctgacaga
    53401 cctgagagtt ggcagaataa acgggggata acagctcggt tatcccctca gaactctact
    53461 gctcgatcac gcatttgctc cataaaggtt ggatcgcatg tcgatctttg ctgtagggga
    53521 atcttttatt ttttttcttc tgttccatca gtaatgtgtc gatttcgtcg ttgacaatgc
    53581 gcgtcacagc gtgacgataa cggtttagcg atgtatcact gatatcctga ggcaggcata
    53641 aactggtatc ggttttgatg cgttcattta tgccatcttt aactttccat ttcagaccat
    53701 caggaacccc ttcctgcaaa tcataggctt tcagtcccgc aagtcgtaca acaacgtcaa
    53761 tcttctctgt tcgtattgtt ttattactta ccaacaaaac tttattagtt tcataccatt
    53821 tttcaaatga ttccagccgt tttactgcat ttatgatgtt attcctaatt ccagaaaatt
    53881 caatagcatc tgtgtatttc attttctttt cttcagcatt aggggctatt ccaggatagt
    53941 caatatcatt ctttacagac tccagatatg catattgcag gcagcctgaa agatgaattg
    54001 ctgcatcctg cagagtatta gtgcgaatca atctaaggag ttcatctcgt tgtatggtta
    54061 tctgacaata atccctttcc catgcggtcg tattctcggt tttttcatct ttatccggag
    54121 taactgttgc gaatttccat gtgtactgga tgaaatctgt tggtttaaaa tatgccatgc
    54181 ttgtttttac ccaaccgcag atttcagctg cataaatatg gacataaata ttgtacattg
    54241 tctttatttc atgctgactg gcgactgaga acatattttt tacgaattct gtacatccga
    54301 ttatcgatag tcgttctctc agtctgtgca catcttcagt atctgacttc ctgcagtccc
    54361 agggccattt gttcttattc tcaaaacagg aaagccattc tggaatattt tgcttatact
    54421 ggtagaattc gttgaccgcc agaccaatgg ttatggcaag tattgttgat tcatctattt
    54481 catgatactg cttcaggtgt gccagcaact ctgaggtgca ggagtgcgca acagacagat
    54541 cctgatggca atacccctgc cgggcttctg ctggaattgg tgaactccag gctttacatt
    54601 cagtcagaat attaaataaa tcatctccta caaactttgc cagttcaggt gttttccagg
    54661 ttcttggtcc caggctgaaa aagtctggtg ttgtctgtac ctcttgcatc gctccccagc
    54721 ttttcaggct gatatttcct tcatcaaagc cattgtataa atggcacata acattaagcg
    54781 cattagtaag agcagtgggt tgagacgatg tagatgcgcc aagagcaaaa ttttcttttc
    54841 tttgtttgtt aaaaattttc tggagctctg tattatcacg caatgccaag agaactttgt
    54901 gctgttcttc aaaccagggg cgatattctg gatcctgata tagctgacgt gccttaaaaa
    54961 tgagcaaaag gagttgttcg gaaacagcct catatatgcc agggaatggg cgggattctc
    55021 cggataaaat acgatagtac tgattttgtt gcatagtctg atttaccatt atgtaagaag
    55081 gtttcctttt gccgcatacc ggatactggc agttaaaatc gatgttatct cctgaaagga
    55141 tacgttgata ctgagtctgc atctactaat aaaacggatg cctgccgtgt tttcggggtg
    55201 atacagggta tacctgagaa tgcatctatt attacgataa ttttcctcca gcgtgttgta
    55261 acaattataa cacccgtcag atctccttct tcttcctcct cctgaatatt ccgatctgtt
    55321 gaagctgttg ttgtcatttc aatgacaggt aaaatgcctg gctctctttc cctcctattg
    55381 tgaatagtaa aaactaattt atttttgtga tcttggtcac aaatttaacc ccctccttct
    55441 cttctttact atcactgtca gaagtttatc tgagcataac atgaagtcga tactagattc
    55501 attttgtgtc attgtcaaca gtgaaaacca cttcttggct taaaatcatt gtttgtaatg
    55561 tgcattattg gttgttttgt tgctaatcaa cacatattga cctatgagtg ggcttgtgag
    55621 tcaagtattg ttttatttgt cttaacttga cttgatttgt tttcgtatgt accttgctgt
    55681 ccatgggtgt tctgtgagca atatgacaac ggaatctcca ctatggattg tccaggaaat
    55741 tcagtcattt catgctcaca actgagataa gaatcattga tatatcagga gggccgtcat
    55801 gtgggttcca ttaacagatg agcaacgttc agtaattttg aaagcctatg ggactgaggg
    55861 tgacagactg gtacgtgaaa aggaacgaaa agagatcacc tcaatttccc gttcacaagc
    55921 ctggaaactt gaacgagagg gaaactaccc accgcgtaag actatcggaa agaaatcttg
    55981 tggctggtta ctaagtgacc ttctctggtg gattcagacc agatgaactg tttgtatttt
    56041 cagtctgaat agcagctatt ttcgctattg tagcggaaat tgctgcaact ttactaaccc
    56101 attaatgaaa atggaggctt tatgtgctta ctagcaccag aaaatcctta tccgatatat
    56161 gcactaccac cgctggtgag aaatgcaata attgaaactc aacaaaatac acaggctcct
    56221 ttggctatgg tggcgacatc cgcattaaca gcgatgtcaa ttgcctgtca gaatcagatt
    56281 gacgtgtgca gacctggaaa tttacgtggg cccgttaata tttactctct gattctggct
    56341 gattccggtg aaaggaaaac gaccgtggat aaagtgttta tgaaagcatt ttatctcagg
    56401 gatgaagccc tggcggaaga atacgcgaaa ctggttgaga attacagtac agaaaaggaa
    56461 atatgggagc aaaaacaaaa agcgctggaa tcaaaatttc ataaagagat tcgtgccggt
    56521 aaagattaca aggcaacaga atcagagctt gaaacgcatc tgaataactc tcctgttccg
    56581 ccgcagatac gtcgaacaat ttttaatgat acaacgatag agggaatgtt aaaatattac
    56641 tccgatagca atcgttcttt tgctcttgta tccagtgaag ggggagtaat ttttgacagc
    56701 cgggccatga gtaaactggg aattattaac actttgtggg atggaggttc tcttttcatc
    56761 gacaggaaat catctcccgg aattaatttg aaggagccaa gactgacgat gtcggcgatg
    56821 attcagcctg atgtttacca caaaggtttt tgtacgcgaa aaaaagaact tgtgaaaacg
    56881 tcaggacatc atgcaaggtt tttgatgtgt caaccaacat caatgcaggg gacaaggata
    56941 ataaccggag ataattattc atcacagtat caggagttat ttgataagag gatcaatgaa
    57001 cttattgatg aaagtctggc gatgagtggt gaacgacgtt gccttcactt ttctcctcag
    57061 gctgccagaa tctggacgga ttattacaat gatgtggaat cgaaactggg gggattgggg
    57121 cctttaagac attgtcggga atatgctgcc aaaaatgcag agtatatggc aagactggca
    57181 ggacttattt accattcgag cggtgaagag ggggagattt ccccttacac tgcagaaatg
    57241 gcgagagaat tagcaatatg gtacggtaat gagtatatgc ggttgtctaa tccgttaact
    57301 tttgacaaca ctgctctgac cgtacctgtg cgactcattc cagaggagct tgaacttttc
    57361 aactggataa aaagctattg cattgagaag gggatcctct gtatgaaaaa aaatgacatt
    57421 ttacagcgtg gtccgaaccg tttccggaaa aaggataaaa tcaactggtt actggattta
    57481 ctgtatgaac aaaacagagt tgtaccggtt attgagggaa aaacgttgtg tgtcgcacct
    57541 aactttgacc tctgaacgac ttaatcgttg agtaacatat agtctcctcc gttacttgcg
    57601 gaggagatta atgccatgac catgtcagtg ttgtattaca gaactttggt acaggtcagt
    57661 cagtaaatat aactggtgta actactattt actattggat agagtgttgt acaggatatt
    57721 gctggctttg gccttagata aggagggcac ttcattgttt tcgtattcaa taaataattt
    57781 aggttaatta gtattattaa tgggagttat gttatctgtt atgttgtctc tttctatcgt
    57841 aatgtcactc ctctttatgc tgagtattct gtatgtgcgt aatgcgtcta tccccatatg
    57901 ctacagtgtg gtggtgtatg gggattagtt atgtgtaaat tatgtcactc actcatagcg
    57961 taatattgag aaagttaatc cattgagtgt gtgtctggtt cgtatttaac actagttacg
    58021 taccagaaca ctttagggat atattatctt tggttcgtac gttatagtag cgttacagtt
    58081 tataagtatg ggtaaatata atcttttctg tatgaaataa tcttcttatg gtgtgtgggg
    58141 tatcaatagc ggtgttgcct gtagctaccc gatactgact tgtgtgaatg tcagattact
    58201 tcctgtaagt gattgaatgg tacttcctta aacttaattc tatgaggtgt gtttttacca
    58261 ttgtaattcg tactggtcct cagtttaagt gagtctcact gtattctgaa gtattacctc
    58321 attgctgtaa tgaggtaata caaaacagtt ttaacaggtt gtctgtactg ctggttatct
    58381 cttttattaa tgccgtggta cgacgtttct gtaccctcag catttacata atttcctctt
    58441 cctgtgagtt ttataaagta aggactataa aatgtataat gctaacccca attacgaaat
    58501 gaatttcgcg attctgaaag atgtgaatga acacatggaa ggtctgtttc aacgtttttc
    58561 taaactgtta cctttcagaa ttgattttgc ctacaggaaa gacacgccca gttttggtca
    58621 ttcctgtaag cacagtatgt gtatggaaat ataccgttta cttagcgaaa cccaaacgat
    58681 gctggcggga tattactggg taatggaata tacaccagac aaagggcttc atattcattt
    58741 tatcggctat ctggatgggc aacgtcacaa gaactcttat cagatatcca ggcaactggg
    58801 ggatatctgg aggcggatca cggaagggga gggttatttt catctgtgcc gggctaaaga
    58861 caaatacccg gtgcgtatcg atcacgtgat tcactatagt gataaatctg cggtagacga
    58921 cttacgttac gccctcagct acctggctaa acaggatcag aaagaacacg gtattattct
    58981 gaggcgtagc aggcttcccg aaaaaagtaa ccggggccga ccgcgactgg acagtaccct
    59041 gtcggagata tgttctctgg tgtaagcccc tgaacgacaa ctgcgggctg gcgctaattg
    59101 ttacagtcgg ttccggaggc catttcagcg acactgcgca tttttacgat gtggctattc
    59161 cgtatgaacc atacggagat ttaaccatgg cacattcttt accattcctt ccggcaaccg
    59221 atacagcaca tcccccgatg tccatccctg cctgctgaca gttttttacg caggtgggcc
    59281 attgtgtgac aaccacagtg cggtaacagc aaccagcctg ttttacccaa actccagtcc
    59341 agattttaac tgaaccgcca ttcacaccct gaccactctg cccgtaccgg gacagctgtt
    59401 gcctgcgtgt cgtctggcgg ctgaggaata tgacgatgta cgcaaaatcc tttattgcat
    59461 ttgatggcaa cgggcgtctg acgggtgccc gtaccgcaca gactgcacct tacgatcgct
    59521 acacttgcca cctttgcggc agtgcgctca gataccaccc gcaatacgac actgagcgtc
    59581 cctggtttga acacactgac gacgggctga cggagcacgg tcagcagtgc ccttatgtca
    59641 gaccggaacg cagggagata caactgatta aacgtctgca gcaattcgtc ccggatgcct
    59701 tacccgtagt acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg
    59761 agcagtactg cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa
    59821 cggtataaaa acggggctgg ggatcacgcc gggtgagcac attatcagtg ccgactctgc
    59881 gctcagcaga aatatccggc agtgtttctg cctttcctgt cgtggtcggt tgattttgca
    59941 gacagatgct cagggggcgt ggtttgagca tgattcacat gccctgagcg cgcaacagaa
    60001 ggcgacctgc gtggtgctga atccggaaaa gtcccaccct tacattgagg atatggcgat
    60061 gtttctatcg ccattaccgg tcgtgctgga gtagcattgt gtgatgtgtg agcagttttt
    60121 tcatgggaag aaattctgtg aggcctgtgg tacggggatt tattgcagag caacatgtac
    60181 gaagtctgtt tacaggtacc agccagattt gtgccaggac tgcggaggtg cgagtactta
    60241 atccgaaatg cccgacctgc tcagggcgtt cagaggtatc ggaacctgca ttttatctgg
    60301 tgtttcgcac ccaccccgtt cgtgacacaa aggcgcgaca ccggatatcc gcagtcctgc
    60361 ttacctgaat aaaacccggg gtattgattg tgtactgtgg tgcgaaatcg caccgtgatc
    60421 gcggattcgc cgaatccgca ctgtggtgtc aaccaaccat acgcgagagt cctctccgta
    60481 tttatcagtg cctcaggacg atttctgcat agttaatcca gggggctgca ttgtttattt
    60541 ccctgctaat ggacgtgaca tccaggatat tcatgatcct gatttccctg agcagtatca
    60601 gcgcttattg gagcgtctgg attatcagac gatgcccggt actaaagcat caggccagcg
    60661 taactctggc tacagcggtt tttgacctgt tgtaactgta aggtgattta ttatgtatac
    60721 agaatcaaat gttaagcttt cctgtaacgc cctctggcaa aagtgtgtcc ttgcaactct
    60781 gtatcgggct gcagccacac atcctgaaat taaattatta caatttaaat taataccagg
    60841 gcagggtatt aataaggcca ttccctctct gatggctgat tttactggtg aactgcatag
    60901 actcatttct gaatatttgc agtgtaagcg acgtaaagat caatctgttg agcccacccc
    60961 tttatattat ctctggagta gggaagctgt ggctaacgat accactcctt atcttatggt
    61021 gctgctactt aataacgatg tctggtgtaa gagtcgtgag gtggtggttc tcagaaatct
    61081 gattgagcag gcgtggggaa aagtattggg tgaagataac tccattacac atctggttgt
    61141 gtatcctgaa gtgctgtcgt tggtatttga tgcagctaac acatacaacg ctgagcctgg
    61201 tgtactggaa cagacggccc gggcttacat tgaatcacat ggtaccattg aacgtcatct
    61261 tgggtgtagt gacttttaac gtcaggtgac gcattgtcac ttctgacctg ctacctgtac
    61321 gccggaatat aatggtcaga gaggcatatt tcgacgcccc taatctgggg cgtttgattc
    61381 aggatgtctg gaacaatccg ggctttgtgt caccgggcgc atcaaagcag taccac
//
LOCUS       DAZGTJ010000021        57365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000021 DAZGTJ010000000
VERSION     DAZGTJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.021"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(105..2384)
                     /gene="maeB"
                     /locus_tag="ACHK8E_001648"
     CDS             complement(105..2384)
                     /gene="maeB"
                     /locus_tag="ACHK8E_001648"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP0343932.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2673..3623
                     /gene="tal"
                     /locus_tag="ACHK8E_001649"
     CDS             2673..3623
                     /gene="tal"
                     /locus_tag="ACHK8E_001649"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0343933.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3643..5646
                     /gene="tkt"
                     /locus_tag="ACHK8E_001650"
     CDS             3643..5646
                     /gene="tkt"
                     /locus_tag="ACHK8E_001650"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0343934.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5749..6792)
                     /gene="ypfG"
                     /locus_tag="ACHK8E_001651"
     CDS             complement(5749..6792)
                     /gene="ypfG"
                     /locus_tag="ACHK8E_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP0343935.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6918..7493)
                     /gene="nudK"
                     /locus_tag="ACHK8E_001652"
     CDS             complement(6918..7493)
                     /gene="nudK"
                     /locus_tag="ACHK8E_001652"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP0343936.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7561..9540)
                     /gene="aegA"
                     /locus_tag="ACHK8E_001653"
     CDS             complement(7561..9540)
                     /gene="aegA"
                     /locus_tag="ACHK8E_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP0343937.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9746..11446
                     /gene="narQ"
                     /locus_tag="ACHK8E_001654"
     CDS             9746..11446
                     /gene="narQ"
                     /locus_tag="ACHK8E_001654"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP0343938.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQNIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11610..14723
                     /gene="acrD"
                     /locus_tag="ACHK8E_001655"
     CDS             11610..14723
                     /gene="acrD"
                     /locus_tag="ACHK8E_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP0343939.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHK8E_001656"
     CDS             complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHK8E_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP0343940.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15262..15618
                     /gene="yffB"
                     /locus_tag="ACHK8E_001657"
     CDS             15262..15618
                     /gene="yffB"
                     /locus_tag="ACHK8E_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP0343941.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
     gene            15622..16749
                     /gene="dapE"
                     /locus_tag="ACHK8E_001658"
     CDS             15622..16749
                     /gene="dapE"
                     /locus_tag="ACHK8E_001658"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP0343942.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16777..16977
                     /gene="ypfN"
                     /locus_tag="ACHK8E_001659"
     CDS             16777..16977
                     /gene="ypfN"
                     /locus_tag="ACHK8E_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP0343943.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17058..17756)
                     /gene="ypfH"
                     /locus_tag="ACHK8E_001660"
     CDS             complement(17058..17756)
                     /gene="ypfH"
                     /locus_tag="ACHK8E_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0343944.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(17830..19845)
                     /gene="tmcA"
                     /locus_tag="ACHK8E_001661"
     CDS             complement(17830..19845)
                     /gene="tmcA"
                     /locus_tag="ACHK8E_001661"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP0343945.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            complement(19860..20723)
                     /gene="ypfJ"
                     /locus_tag="ACHK8E_001662"
     CDS             complement(19860..20723)
                     /gene="ypfJ"
                     /locus_tag="ACHK8E_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP0343946.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(20891..21604)
                     /gene="purC"
                     /locus_tag="ACHK8E_001663"
     CDS             complement(20891..21604)
                     /gene="purC"
                     /locus_tag="ACHK8E_001663"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP0343947.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(21817..22851)
                     /gene="bamC"
                     /locus_tag="ACHK8E_001664"
     CDS             complement(21817..22851)
                     /gene="bamC"
                     /locus_tag="ACHK8E_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP0343948.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(22868..23746)
                     /gene="dapA"
                     /locus_tag="ACHK8E_001665"
     CDS             complement(22868..23746)
                     /gene="dapA"
                     /locus_tag="ACHK8E_001665"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP0343949.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            23892..24464
                     /gene="gcvR"
                     /locus_tag="ACHK8E_001666"
     CDS             23892..24464
                     /gene="gcvR"
                     /locus_tag="ACHK8E_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP0343950.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24464..24934
                     /gene="bcp"
                     /locus_tag="ACHK8E_001667"
     CDS             24464..24934
                     /gene="bcp"
                     /locus_tag="ACHK8E_001667"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP0343951.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(25033..26094)
                     /gene="yfgO"
                     /locus_tag="ACHK8E_001668"
     CDS             complement(25033..26094)
                     /gene="yfgO"
                     /locus_tag="ACHK8E_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0343952.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            26307..27770
                     /gene="bepA"
                     /locus_tag="ACHK8E_001669"
     CDS             26307..27770
                     /gene="bepA"
                     /locus_tag="ACHK8E_001669"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP0343953.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            27791..28150
                     /gene="arsC"
                     /locus_tag="ACHK8E_001670"
     CDS             27791..28150
                     /gene="arsC"
                     /locus_tag="ACHK8E_001670"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP0343954.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(28256..28957)
                     /gene="hda"
                     /locus_tag="ACHK8E_001671"
     CDS             complement(28256..28957)
                     /gene="hda"
                     /locus_tag="ACHK8E_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP0343955.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(29052..30341)
                     /gene="uraA"
                     /locus_tag="ACHK8E_001672"
     CDS             complement(29052..30341)
                     /gene="uraA"
                     /locus_tag="ACHK8E_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP0343956.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            complement(30427..31053)
                     /gene="upp"
                     /locus_tag="ACHK8E_001673"
     CDS             complement(30427..31053)
                     /gene="upp"
                     /locus_tag="ACHK8E_001673"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP0343957.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            31378..32415
                     /gene="purM"
                     /locus_tag="ACHK8E_001674"
     CDS             31378..32415
                     /gene="purM"
                     /locus_tag="ACHK8E_001674"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP0343958.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            32415..33053
                     /gene="purN"
                     /locus_tag="ACHK8E_001675"
     CDS             32415..33053
                     /gene="purN"
                     /locus_tag="ACHK8E_001675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP0343959.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            33224..35290
                     /gene="ppk1"
                     /locus_tag="ACHK8E_001676"
     CDS             33224..35290
                     /gene="ppk1"
                     /locus_tag="ACHK8E_001676"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP0343960.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            35295..36836
                     /gene="ppx"
                     /locus_tag="ACHK8E_001677"
     CDS             35295..36836
                     /gene="ppx"
                     /locus_tag="ACHK8E_001677"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP0343961.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(36875..39118)
                     /gene="pdeF"
                     /locus_tag="ACHK8E_001678"
     CDS             complement(36875..39118)
                     /gene="pdeF"
                     /locus_tag="ACHK8E_001678"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP0343962.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(39300..39452)
                     /locus_tag="ACHK8E_001679"
     CDS             complement(39300..39452)
                     /locus_tag="ACHK8E_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343963.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     YVIMMS"
     gene            39470..39661
                     /gene="yfgG"
                     /locus_tag="ACHK8E_001680"
     CDS             39470..39661
                     /gene="yfgG"
                     /locus_tag="ACHK8E_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP0343964.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            39972..40490
                     /gene="yfgH"
                     /locus_tag="ACHK8E_001681"
     CDS             39972..40490
                     /gene="yfgH"
                     /locus_tag="ACHK8E_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0343965.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            40506..41045
                     /gene="yfgI"
                     /locus_tag="ACHK8E_001682"
     CDS             40506..41045
                     /gene="yfgI"
                     /locus_tag="ACHK8E_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP0343966.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(41138..42715)
                     /gene="guaA"
                     /locus_tag="ACHK8E_001683"
     CDS             complement(41138..42715)
                     /gene="guaA"
                     /locus_tag="ACHK8E_001683"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP0343967.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(42784..44250)
                     /gene="guaB"
                     /locus_tag="ACHK8E_001684"
     CDS             complement(42784..44250)
                     /gene="guaB"
                     /locus_tag="ACHK8E_001684"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP0343968.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            44412..45788
                     /gene="xseA"
                     /locus_tag="ACHK8E_001685"
     CDS             44412..45788
                     /gene="xseA"
                     /locus_tag="ACHK8E_001685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP0343969.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(45857..53908)
                     /locus_tag="ACHK8E_001686"
     CDS             complement(45857..53908)
                     /locus_tag="ACHK8E_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFP0343970.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(54029..55006)
                     /locus_tag="ACHK8E_001687"
     CDS             complement(54029..55006)
                     /locus_tag="ACHK8E_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFP0343971.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(55061..57238)
                     /gene="sinH"
                     /locus_tag="ACHK8E_001688"
     CDS             complement(55061..57238)
                     /gene="sinH"
                     /locus_tag="ACHK8E_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFP0343972.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT        13994 a        15112 c        14362 g        13897 t
ORIGIN      
        1 tgattttttt attattcgta aactcagtaa taagctttac taatatattg cagctaaact
       61 gcttaccctg aatattcagg gtaagcgtga gagttaaaaa aaaattacag cggttgggtt
      121 tgcgcttcaa ccacggccag cgccaccata ttgacgatac gacgtaccga tgcgatcggc
      181 gttaacacgt gaaccggttt cgccacaccc atcagcaccg gaccgacagt cacgccttcc
      241 gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt cggcatcacc
      301 agaatattag cggaaccttt caaagggctg tccggcatac ggtcattccg aatcgcttcc
      361 accagcgctg catcgccgtg catttcacca tcaatcatca gttctggtgc acgttccctg
      421 accagttcca gcgcctgacg cattttgctc gacgacgggc agtcagaaga accaaagttg
      481 gagtgcgaca acagagccac acgcggctca ataccaaaac gacgaacagt ttctgccgcc
      541 atcaaggtga tctccgccag ctcttctgca tcaggctcat cattgacgta ggtatcggca
      601 ataaaggtgt taccactcgg caacagcagc gcgttcattg cacctgcggt gtgaacgcca
      661 tcgcgataac caaagacatt tttcaccacg ctaaagtgtt catgataatc gcccaccgta
      721 ccgcaaatca ttgcatcggc ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg
      781 ttactgatca gcgcacgctg cgcctgctcc tgagtgacac cgcgacgctt catgatctgg
      841 aagtattcgg tccagtactc tttaaagcgc ggatcggatt cgttattgac gatctcaaaa
      901 tcaacgcccg ccttgatctg caagcccagt ttctgaatgc gcatttcgat cacgttcgga
      961 cgaccaataa ggatcggttt cgccagtccc agcgttacca gttcctgagt ggcatgcaga
     1021 acgcgcgcct cttccccttc cggcagaact acgcgcttcg gcgctttgcg agcctgggag
     1081 aaaatcggct tcataaacag gttagttttg taaacgaact ccgtcagctt gtcgatgtag
     1141 acgtcgaaat cagcaatcgg acgcgtcgcc acgcccgact ccatcgccgc tttcgccacc
     1201 gcaggagcga tcttaacgat caagcgcgga tcaaacggtt ttggaatgat gtattccgga
     1261 ccaaagctca gatcctgatc gccatacgct gaagccacca cttcactctg ttccgcatgg
     1321 gccagttctg caatcgcgcg taccgccgcc agtttcatct cttcgttgat ggcggttgcg
     1381 ccaacgtcca gcgcgccacg gaagataaac gggaagcaaa ggacgttgtt cacctggttc
     1441 gggtagtcgg aacggccggt acagatgatg gcatccggac gcacttcttt cgccagcggc
     1501 ggcagaattt ccggttccgg gttcgccagc gccaggatca ttggcgcacg ggccattttc
     1561 ttcaccattt cctgggtcag cactttcggg ccggaacagc cgaggaaaat atccgcgcct
     1621 tcaatcacat catcgagggt acgtttaccg tcatccacca ccgcgtatgc ggctttggtt
     1681 tccgccatgt ttggctcacg gccccgatag ataacgcctt ttgaatcgca aaccacgatg
     1741 ttatgttttt gcagacccag cgctaccagc aggttcatac aggcgattgc tgcggcaccc
     1801 gcgccagaaa ccaccatccg cacgtcggag atgtttttct ccaccacgcg caaaccgttg
     1861 aggatggcgg cagtgctgat aattgccgtg ccgtgctgat cgtcgtggaa taccggaata
     1921 ttcatccgct cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg
     1981 aggttgatgc cgccaaaggt cggttcgagc gcagcgacga cttcaataaa tttgtccggg
     2041 tcgagttcat caacttctat gtcaaataca tcaatcccgg cgaatttctt aaacagaacg
     2101 cccttgcctt ccatcaccgg tttgcctgcc agcgcgccaa tgttgcctaa ccccagcacc
     2161 gccgtaccgt tagagatcac cgctaccagg ttaccgcgcg cggtatattt gtaggctttt
     2221 aacgggtctt tttcgatttc aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc
     2281 agatcgcgct gcgttgccag aggcttggtt ggagaaacct ggattttccc tggaactgga
     2341 aattcatgga aatcaagtgc actttgtttt aactggtcat ccatttggtt gttcctttca
     2401 cgtaacgttc acaaataaag tgtgtgggca acagcccctg cccacaacgt ggcgcacatt
     2461 attaccttgc cggagtctac agactttgag caagtccaaa ctctcaccat taatataatg
     2521 ttttggtaat aatcctataa cactgatgtt acctgcttaa tccagcaata ccatgcctgt
     2581 ctgctatgct tttttgatgc gtttagcgaa atttctcaga agtgtgaatt aacgcactca
     2641 tctaacactt tacttttcaa ggagtatttc ctatgaacga gttagacggc atcaaacagt
     2701 tcaccactgt cgtggcagac agcggcgata ttgagtccat tcgccattat catccccagg
     2761 atgccaccac caatccttcg ctgttactca aggctgccgg attatcacaa tatgagcatt
     2821 taatagacga tgctatcgcc tggggtaaaa aaaatggcaa gacccaggag caacaggtgg
     2881 tcgcagcgtg tgacaaactg gcggtcaatt tcggtgctga aattctcaaa atcgtacccg
     2941 gtcgcgtgtc aacagaagtt gatgcacgcc tctcttttga taaagaaaag agtattgaga
     3001 aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattctga
     3061 tcaagctggc ttcgacctgg gaaggaattc gcgcggcaga agagctggaa aaagaaggta
     3121 ttaactgcaa cctgacgctg ctgttttctt ttgcacaggc gcgggcctgt gcagaagcag
     3181 gcgtttttct gatttcgccg tttgtcgggc gtatttatga ctggtatcag gcacgcaagc
     3241 cgatggaccc gtatgtggtg gaagaagatc caggcgttaa atcagtacgc aatatctacg
     3301 actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg
     3361 aacaaatcct cgccttaacc ggctgcgacc gactgactat cgcaccgaat ttgctgaagg
     3421 agctacagga aaaagtttcg ccagtggtac gtaaattaat cccaccttct cagacgttcc
     3481 cacgcccagc tcccatgagc gaagcggaat tccgttggga gcacaatcag gatgcgatgg
     3541 cggtagaaaa actgtctgaa ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag
     3601 atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt
     3661 gccaatgcga ttcgcgcact cagtatggat gcggtacaaa aagccaattc tggtcatccc
     3721 ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat
     3781 aatcctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg
     3841 tcgatgctgc tctacagttt gctgcatctg accggttacg acctgccact ggaagaactg
     3901 aagaacttcc gtcagttgca ttcgaaaacc cctggtcacc cggaaatcgg ctatacgcca
     3961 ggtgttgaaa ccaccaccgg tcctcttgga caaggtttgg cgaacgccgt cgggctggcg
     4021 atagcagagc gtacgctggc ggcgcagttt aatcagccgg atcatgaaat tgtcgatcac
     4081 ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatctctca cgaagtctgt
     4141 tcgctggcgg gcacgctggg gctgggcaag ctgattggtt tttacgatca caacggtatt
     4201 tccatcgacg gtgaaaccga aggctggttt accgacgata cggcaaaacg ttttgaagcc
     4261 tatcactggc atgtgatcca cgagatcgac ggtcacgatc cgcaggcggt gaaggaagcg
     4321 atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt
     4381 ggctttggat cgccgaataa agcaggtaaa gaagaggcgc acggcgcacc gctgggtgaa
     4441 gaagaagtgg cattggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct
     4501 aaagagattt atcacgcctg ggatgcccgc gaaaaaggcg aaaaagcgca gcagagctgg
     4561 aatgagaagt atgcagccta caaaaaggcc catccgcaac tggcagaaga gtttacccgt
     4621 cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgaa
     4681 ttacaggcaa atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct
     4741 tacgggccaa tgcttcctga attgctcggc ggttcggcgg atctggctcc cagcaacctc
     4801 accatctgga aaggttctgt gtcgctgaag gaggatccgg cgggcaacta cattcactac
     4861 ggggtgcggg aatttggcat gaccgctatc gccaatggca ttgcgcacca cggcggcttc
     4921 gtgccgtata ccgcgacgtt cctgatgttt gttgaatacg ctcgtaacgc cgcgcggatg
     4981 gcggcactga tgaaagcgcg gcagattatg gtttataccc acgactcaat tggcttaggg
     5041 gaagatggtc cgacccacca ggccgttgag caactggcca gtctgcgctt aacgccaaat
     5101 ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg ttggctggaa gctggcggtt
     5161 gagcgccaca acggaccgac ggcactgatc ctctccaggc agaatctggc ccaggtggaa
     5221 cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtactgaa agacagcggc
     5281 ggtaagccag atattatttt gattgccacc ggttcagaga tggaaatcac cctgcaagcg
     5341 gcagagaaat tagcaggaga aggtcgcaat gtacgcgtag tttcgctgcc ctcgaccgat
     5401 attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct
     5461 cgcgtggcgg tggaagcagg catcgccgat tactggtaca aatatgttgg tctgaaaggg
     5521 gcaattgtcg ggatgaccgg ctatggggaa tctgctccgg cagataagct gttcccgttc
     5581 tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt
     5641 gcctgatggt gatcgccgga tgctatatgt tcccctcacc ctaaccctct ccccagaggg
     5701 gcgaggggac ggtccgtgcg ctttttaggg gcgcatctgg gattttgttt aacgggtgat
     5761 ccacaacgtg ggccaggcgt ctggcccatg ccagttatcg caggtgggtt ctgcggcgta
     5821 acgcaccagg cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct
     5881 taatccgcgc cctttcgcta aggtcaccag ttcacgcgat ttttcatcga atgttgcgtt
     5941 catcagttcc agttcattcg tctcctgacc gctgttgaac ggcaaacgta accgaaccgg
     6001 gcgcgaggcc agtggctttt tacgcgacac aatccatgcc aaatcaatgg tgttataggc
     6061 ccctgcctca cagctaatca tcatcagcgc tttatcatcg gtcagcgcag tgacattcac
     6121 ctctcgacgc aatggatcaa gcgagcagcg cagaccattc atccgccagt ttccataatc
     6181 cagcaaatcg ttgcgttctt cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac
     6241 ctctttcagc gcaggcgcgg gcggtacgct gagcggcggt tcatcccctt tctttatcca
     6301 cgcggtttca ctgccaacgc gcttttgctg agcatctata aacaacaacg ccgcttttaa
     6361 gccactcaga gaaatagtct gattgccgtc gcgtaaggtg atcgccctcc cttcctgaat
     6421 catctgcaaa aacgcggtga tggttgccgt atcatcggtt actaataacc atggtgaaat
     6481 ccgccacttg tcaccactta acgccaacgg ctcaccatct aacagcagcc gtggcgctat
     6541 ctccccttca gacgcatccg gcgacttcaa cccgccgcgc tcaatacgta aaacggcatc
     6601 ggtatgcgcc ccggcgctgc ggctcagggt catcaccagt ccgttatgat cgcccgtatt
     6661 acgcgccacg cagaagtttt ggttattaca ggtgacctgc cagtcggaaa acgcccgttg
     6721 cgctggtgcc gcccacacca aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat
     6781 gcgatagcgc atggacggta cgaccccaga agttcaaaca aacagcacaa gtcgtatctt
     6841 cgttgcgcag gtagcgcggc tcaatcggat ttatcggatt gagccaataa taaacaacga
     6901 aatattattg ttatttttca gtccattaaa tgtgacattt gcaaatagtt aagcaataac
     6961 accgtcttac cgtcacgtat ctcgccagtt ttgatcatct ccagcgcctt gttgaacggc
     7021 agctcgagca cttcaatatc ttcatcttcg acaccaccgc cggcgttagc gcgctggctg
     7081 tcactgtatt cggcgataaa gaagtggatt agctcagtca caccgcctgg cgacatatac
     7141 agttcaaata atttgcgcac ttcgccaact tcataacccg tctcttcaat cgcttcttta
     7201 cgaatacaca cttccggttc gtcgttatcc agcagcccgg cgcaggtttc aatcagttgc
     7261 ccgctttcat tgccattaac ccaggtagcg acgcggaact ggcgaatcag aaccacggtc
     7321 tttttcttcg cgttgtacag gaggatcgtt gcaccattac cgcgatcgta aacttcacgt
     7381 ttatggcgga taacttcgcc atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg
     7441 aaatagttat cggagagaat tttgtcttta atgagggtga tttgttgcgt cataccgact
     7501 ccacagcgcg aaatgaacaa ttatcttacg ctgtgaagcc gggtttgtct gcgcaggcta
     7561 tcagtgagat ttgactgatt ttacccccag ccagtcaata atcccctgtg ccgcatgacg
     7621 tccttctgcc attgcggtaa ccaccagatc cgcaccacgc acggcgtcac caccggcgaa
     7681 gattttcgga ttggtggtct ggtaacggta ctggctttcc acatccgcga tgatgcggcc
     7741 ccatttgtct accgttacac cgtgcgactc cagccacggc atcccgtgcg gattgaagcc
     7801 aaacgccata atcaccgcat cggctggcat gacaaattca ctgccttcca ccggcactgg
     7861 acgccgacgc ccctgggcat ccggctcgcc aagacgcgtg cgcaggaaac gaatcccgca
     7921 gacgtgacct tgttcattca gttcaagcgc caccggctgg acgttaaatt cgaagttggc
     7981 cccctcttca cgggcgttct tcacctcttt cttcgagcct ggcatgttag cttcatcacg
     8041 acgataagcg caggtgacgt tactcgcgcc gtggcgcagc gcagtacgca cacagtccat
     8101 cgcggtgtcg ccgccgccca gtaccacgac gttaagtccg gcagtattga taaacggctc
     8161 ttccggtagc tcttcgagtc ccatcacctg tttagtattg gcgatgagga acggcagcgc
     8221 gtcataaacg cccggcgcat cttcattggg taaacccgct ttcatggagc ggtaagtgcc
     8281 tacgccaacg aagaccgcgt cgtattgttc caaaagcgaa ttcaaagaga catctttacc
     8341 cacttcacaa ttgagttcga agtgaatccc catcgcgctg aagatttccc gacggcgtgc
     8401 cagcagggat ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc
     8461 tggatggcga tcgtacaccg tcacctctac gccattgcgg gtcaaaacat ccgcacaggc
     8521 cagccctgcc ggacccgcac cgataatcgc cacccgcttg tccactttgg tgacatggct
     8581 taagtcagga cgccaacctt tcgccaacgc ctgatctgaa atgtagcgtt caatgttacc
     8641 gatagttact gcgccgtgct catcgcgaat agtacaggca ccttcacaca aacggtcttg
     8701 cgggcaaacg cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa gctcgacggc
     8761 ggcgtcgatg tttccggctt tcaccagttc aatccactgc ggtatatggt tatgcagcgg
     8821 gcaggtccat tcacaaacgc tgtgctcgcc gcacttaagg cagcgcgagg cttcccgttg
     8881 tacctgatcg gcacggaatg gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat
     8941 cgccagttta tctggctcgc cacgcgcggg cgttgcctgc atttgctcga ctttactcat
     9001 taccggcatt tcttgcgccg cggtactggc atgccacggt tgatgttcct gacgtgcggt
     9061 acgcaagcgg cgggatttcg ccatgcctga gagtgcggcg tcagtgacca gttgcagcgc
     9121 gtccgctggg caattctcga cacaagcagg accgttttcg cgccctgcac aaaggtcgca
     9181 tttatgcgcc gtggctttta cttttcctgc cgcgacgggg gtcaggacga tttgcatcgt
     9241 accaaatgga caggccacca cgcaggattt acagccaata cacttttgct gattgacctg
     9301 aatgctgtca tcaacgtggc tgattgcgcc attagggcag ctacgggcgc agggcgcatc
     9361 ttcacaatgg tgacaggtca cggcactacg ttgctgctga tgtttgataa ccgtaattcg
     9421 gggatgaaaa tggtgttggc tcaggacatg ttgctcatca ttgtgagcca tgacacaggc
     9481 gatttcacaa gcatgacaac ccagacattg ctgactgttg gccataataa aacgattcat
     9541 aacgaccttc ttttttggtt gtaaaaacct tattctttat atgagtgttg ttattacccg
     9601 acttacaggg gaatcggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac
     9661 tgtggcagat caaataatcc catcactgac taaattgcgt ttcagcgaat tggaacatta
     9721 atgatttttt gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc
     9781 ccgggccttt ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac
     9841 gctggcgagc agtttacgcg acgctgaggc tatcaatatt gccggatcgc tgcgtatgca
     9901 gagttaccgc ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca
     9961 gttatttcag caggcactgc attcaccggt attaaccaat ctcaacgtct ggtatgtgcc
    10021 agaggcagta aaaacccgct atgcgcatct gaatgccaac tggctggaga tgaataatcg
    10081 gctcagcaag ggcgacttgc cgtggtatca ggccaatatt aataattatg ttaatcagat
    10141 agacctgttc gtgctggctt tacagcacta cgccgaacgc aaaatgctgc tggtggtggc
    10201 gatctccctg gctggcggca tcggtatttt cacgctggtc ttttttactt tgcgccgcat
    10261 acgccatcag gtggttgccc cgctgaatca gctggttacc gccagtcagc gtattgaaca
    10321 cgggcagttc gactcgccgc cgctggatac cagcctgccg aatgagcttg gcctgcttgc
    10381 aaaaaccttt aaccagatgt cgagcgagct gcataaattg taccgttcgc tggaggcgtc
    10441 agtagaagaa aagacccgcg atctccacga ggccaagcgt cgtctggagg tgttgtatca
    10501 gtgttcgcag gcgctaaaca ccagccagat tgatgtgcat tgtttccgcc atattttgca
    10561 gattgttcgc gacaatgaag cggctgaata tctggagtta aatgtcggtg acaactggcg
    10621 gattagcgaa gggcagccaa acccggaatt gccgatgcag attttaccgg tgacaatgca
    10681 agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat cagaaccgct
    10741 gcttaacagt gtttcgtcga tgctgggacg cggtttgtac tttaatcagg cgcagaagca
    10801 ttttcagcag ttattgttga tggaagaacg tgcgaccatc gcccgcgaat tgcacgactc
    10861 gctggctcag gtactttctt acttacgtat ccagttgacg ttactgaagc gttcgatacc
    10921 ggaagataac gccaccgcac aaaatatcat ggccgatttc tcccaggcgc tgaatgatgc
    10981 ttatcggcag ttacgcgagc tgttaactac tttccgcctg acgctgcagc aggcggatct
    11041 cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg ccaaactgac
    11101 cctcgactgc cgtctgccaa ccctggcgct ggatgcgcaa atgcaggtgc atttgttgca
    11161 aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt
    11221 tagctgcgtc accgcgccgg atggcaatca cacagtctat attcgcgaca acgggattgg
    11281 catcggtgaa ccgaaagaac ccgaaggcca ttatggtctg aatatcatgc gcgaacgcgc
    11341 ggaaagattg ggtggaacgc tgactttttc acaaccttcc ggcggcggca cgttagtgag
    11401 tattagcttt cgctctgcgg agggtgagga aagtcagctg atgtaatgcc tcttattgac
    11461 caaagaatag tggcacttaa ggttcagtat aaaagggcat gataatttac attaactcct
    11521 ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac
    11581 gatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt
    11641 ttgcctgggt gctggcaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc
    11701 ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg
    11761 gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg
    11821 gcctcgataa tctcatgtat atgtcgtctc agagcagtgg caccggccag gcatctgtca
    11881 ctttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc
    11941 tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta
    12001 aaaccggcga taccaatatt ctgaccattg ccttcgtctc taccgatggt tcgatggata
    12061 aacaggatat tgccgattac gttgccagta atattcagga cccgttaagc cgcgtgaatg
    12121 gcgtcgggga tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg
    12181 cgaaactcaa cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga
    12241 acgcgcagat tgcggttggg caacttggtg gtacgccttc cgtcgataag caggcgctca
    12301 acgccaccat taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct
    12361 tgcgggtgaa tcaggacggt tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg
    12421 gggcggaaaa atacgattat cttagccgct tcaatggtaa gccagcctcc gggctggggg
    12481 taaaactggc ctccggcgcg aacgaaatgg cgactgcgca gctggtgctc aatcgtctcg
    12541 acgagctggc gcagtatttt ccacacggac tggaatacaa ggtggcgtat gaaaccacct
    12601 cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg
    12661 ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg
    12721 ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta tgccttcggt tacagcgtca
    12781 acaccttaac catgttcgcg atggtgctgg cgatcggtct gctggtggat gatgccatcg
    12841 tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca
    12901 cacgtaaatc gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg
    12961 cggtatttgt gccaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct
    13021 ctattaccat tgttgcggcg atggtgctgt cagtactggt agcgatgatc ctcactccgg
    13081 ctctgtgtgc cacgctactt aagccactga aaaaaggtga gcatcacggg caaaaaggct
    13141 tttttgcctg gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg
    13201 cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca
    13261 tggtgttcct gttcctgcgt ttgccgacgt cgttcttgcc acttgaagac cgtggcatgt
    13321 ttactacctc ggtacagttg cccagcggtt caacccaaca acagaccctg aaagtcgttg
    13381 agcaaatcga gaaatactac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca
    13441 ctgttggttc tggccctggg ggtaacggac aaaacgtggc gagaatgttt atccgcctga
    13501 aagactggag cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa
    13561 cgaaggcgtt taacaagatt aaagaagctc gcgttatcgc cagcagcccg ccagcaatta
    13621 gcggtcttgg tagttctgca ggttttgata tggagttgca ggaccacgct ggagcgggtc
    13681 acgatgcgct gatggcagca cgtaaccagt tgctggcgct ggcggcggaa aacccggagc
    13741 taacccgtgt gcgccataac ggcctcgacg acagtccgca gttgcagatt gatatcgacc
    13801 agcgtaaagc tcaggcgctg ggcgttgcta tcgacgatat taacgacaca ctgcaaaccg
    13861 cctggggttc gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg
    13921 tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa
    13981 ataaagatgg cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gagacaggct
    14041 cgccgcgtct ggaacgctat aacggttatt ctgcggtaga gattgtaggg gaagccgcac
    14101 cgggggtcag taccggtacg gcgatggata ttatggaatc gttagtgaag cagttgccaa
    14161 acggctttgg tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg
    14221 ctccggcgct gtacgccatt tccttgctgg tggtattcct gtgtcttgcg gcgttgtatg
    14281 aaagctggtc ggtgccgttc tcggtaatgc tggtcgtgcc gctgggggtt atcggcgcgc
    14341 tgctggcaac ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa
    14401 cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga
    14461 accaaaaagg ccacgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc
    14521 cgattctgat gacttcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg
    14581 gtgccggttc cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcgg
    14641 ccactattct ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc
    14701 cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac atgctaatgt gtcgcctttt
    14761 tcaactttcc gattaagaac ctgctcagcg ggttcttgct gtttgtactt tgtctcagga
    14821 attacttacg aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca
    14881 taacaacctc tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa
    14941 tttactcgat tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc
    15001 ctgcggtgat aatttgtatg tgatacacag caacatttcg agatattcat acggcatcta
    15061 atactgattt aattctggtt aaaatacaga cagataacaa gatgaatatt cttaatgttt
    15121 acgttaaaaa tgtttaatgt tatttaatag ttgttaattt gaatacttcg ataatgttat
    15181 atttcctgat gatcatttgc aggcaaaatg ttttcaccct taaatgagta tttattctca
    15241 taaatcgaaa aaggattcat tatggttaca ctttacggca tcaaaaattg tgacaccatt
    15301 aaaaaggctc gccgttggct ggaagccaat aacatcgact atcgctttca tgattaccgc
    15361 gtcgatggat tggacagcga attattgaac ggttttatca acgaattagg ctgggaagcg
    15421 ttactcaaca cccgtggtac aacctggcgt aaactggacg aaaccacccg caataaaatc
    15481 accgatgcgg cctctgcggc gacattaatg actgaaatgc ctgcaattat caaacgtcca
    15541 ttgctctgcg cgcctggtaa gcctatgctg ctgggtttca gtgaatccag ttatcagcaa
    15601 tttttccatg aggtgtagtc tatgtcgtgc ccggttattg agctgacaca acagcttatt
    15661 cgccgccctt ccctgagtcc tgatgacgca ggatgccagg ctttgttgat tgaacgtttg
    15721 caggcgatcg gttttaccgt tgaacgcatg gactttgccg atacgcagaa tttttgggca
    15781 tggcgtgggc agggtgaaac gttagccttt gccgggcata ccgacgtggt gccgcctggc
    15841 gacgccgacc gttggatcaa tccgccgttt gaacccacca ttcgtgacgg catgttattc
    15901 gggcgcggtg cggcagatat gaaaggctcg ctggcggcga tggtggtagc ggcagaacgt
    15961 tttgtcgcac aacatcccaa ccatacgggg cgactggcat ttctgatcac ctctgatgaa
    16021 gaagctagtg cccacaacgg tacggtaaaa gtcgtcgaag cgttaatggc gcgtaatgag
    16081 cgtctcgatt actgtctggt tggcgaaccg tcgagtatcg aagtggttgg tgatgtggtg
    16141 aaaaatggtc gtcgcggatc gttaacctgc aacctcacca ttcatggcgt gcaggggcat
    16201 gttgcctacc cacatctggc tgacaatccg gtacatcgcg cagcaccttt ccttaatgaa
    16261 ttagtggcta ttgagtggga tcagggcaat gaattcttcc cggcgaccag tatgcagatt
    16321 gccaatattc aggcgggaac gggcagtaac aacgttattc cgggtgaact gtttgtgcag
    16381 tttaacttcc gcttcagcac cgaactgact gatgagatga tcaaagcgca ggtacttgcc
    16441 ctgcttgaaa aacatcaact gcgctatacg gtggattggt ggctttccgg gcagccattt
    16501 ttgaccgcgc gcggtaaact ggtggatgcg gtcgttaacg cggttgagca ctataatgaa
    16561 attaaaccgc agctactaac cacaggcgga acgtccgacg ggcgctttat tgcccgcatg
    16621 ggggcgcagg tggtggaact cgggccggtc aatgccacta ttcataaaat taatgaatgt
    16681 gtgaacgctg ccgacctgca gctacttgcc cgtatgtatc aacgtatcat ggaacagctc
    16741 gtcgcctgat gagtggttct gcaagaggaa ataagcatgg actggctggc taaatattgg
    16801 tggattctgg tgattgtctt tttggtaggc gtcctgctga acgtgattaa agatctcaag
    16861 cgcgtcaacc ataagaaatt tctcgccaac aagccggagc ttcccccgca tcgtgatttc
    16921 aacgataagt gggacgatga cgacgactgg ccgaaaaagg atcaaccgaa gaagtaagct
    16981 aagtggccgg ataaggcgtt aacgccgcat ccggccatga tgcctgatac agtcttatca
    17041 gacctacccg gctattatta catcatttca atcacatcat catcgccagg cttaccgccg
    17101 cttagcgctt cgtcgaaata atgtttcgga atggtataac gcaagtgatc cagagcaaat
    17161 tgcatactgc gattgtcgat cgcatggcct aaatcctcca cgatatccag cgtcacgtca
    17221 ccaccggcac tgattaacgc ttcctgcgcc gcaaccgcat gcgccagatc gataaccgga
    17281 tcttcaccgc catgaatcag gtgaattgtc gtggctgtag acgcagtttc cggcaggctc
    17341 gcatagcgtc cgttaaaggc aatgacgcgt gaggcaaggc ccggttcggc tttaatgctc
    17401 tccagcgcca taatcgcgcc ttgagagaaa ccgattaacg ccgtggcggt agcccctact
    17461 ccgctctgtt tctgccagta gcgcaccgtc tcaataaacg tcggcatgat ggcatccaca
    17521 cgcgcctggc gattatcttc cgtaataccc tgcaccgaaa accactgacg ccccgccgga
    17581 ttaccgctcg gctccgcgcc tccgacactc accaccagcg catcaggaaa tagcggcgca
    17641 aaccagctac ctatttcccc cattgccacc gggttatccc cgacaccgtg gaaaagcagt
    17701 aacaactgtt gcgcaggttt atccgggctt tgaacaacaa aatggtcatg tttcatggcg
    17761 aactccttaa ctgatgtcat taattttacg ccgctgactc acaatgacca tgggagaaaa
    17821 ttgaaggagt cagtgaaaaa attgccattg cgttatgcga tcgcgcaaac gctcagtgcg
    17881 aacatcatca agtgcgtata gcgcctgcgc ggcctcttcc cgctgacgga ccagtaacag
    17941 cttacggcct gaaagtttaa gtgtggtaca taactgcgcg tcgctggcgt ttttctgtaa
    18001 acgcccacgc agcgccggta atgccatttc gctggtttgt aacagacgca ttaagcaacc
    18061 taacgacgtt aatagcggac gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc
    18121 ggcagaaagc acgatatcgt ttagtggatc aacagggagc gtttcgccat tccactttgc
    18181 gagagcttgc gcatcgcgac gtaaacggta atgctcacgt tcagccagct gtttacccgc
    18241 atcactcatc ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt cacgatgatt
    18301 acccattcgc accagcacaa aaccgcagcg ttgccagaaa cgccataatt ccccggtgta
    18361 accaaaactc accgaaagat agtcgaggtc atgcgtatat tgcaaagcac cagcaatcag
    18421 ttgctgacct gtgccttccc gctgacgagc cggatgaact gctatccggc taacccgcct
    18481 tccgcgcaat gtcgccgcca gtggattgct gccgtgcgcc gccagcgact gggccaccag
    18541 attaccccgc gggcgacgaa aacctgccca taccgcctga ctgagttctt gagataatcc
    18601 gccctcatcc accagccaca gcgccccggc aatcgcgttt tcgccagccg cctgtaaaaa
    18661 atgttgccct ggtgcatcca tcatgcggcg taaatccagc ggcgaagtcc ggtagtgcgc
    18721 accagacaat aattgataaa cctttaacgg cgtttgtggc tcgcttcgcc ataacgtctg
    18781 ttcaaatgcg gaaatgacga tattgccttg tggtgtgtgg gtgaagtttt catcgtcaaa
    18841 aaccagtgcc tcactaacca ttttttccag cgggcatccc tgtgcccagc ggatcggctg
    18901 ttgcagttca aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg
    18961 tcccgtgcct tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac
    19021 cagttgatgc aacaacggcg caggtatggc tgcggcttca tcgaccacca gccagtcggc
    19081 ttgctcatcg ctggctaaca aggcatccgg cgcaataaag cgaaacttct cgcccgcaaa
    19141 ttgtgccagt acatacgttg ccgcttttgc gggcgcggtg acaatcgcac tacccgcaat
    19201 acgagaaatg agttgccctg ccagcgccga tttaccgcgc ccacgcgcag ccgttaccac
    19261 cgccacgccc aatggcatgg tcagtagctg ctgtaagagt tgctgttgtt ctggctgtgg
    19321 tgcgccggtc gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg tgaacggctg
    19381 gttctgccgc cagaggatag cgtcgttatt cgccgtaagt acgcgtttga gatgctggac
    19441 aaaatgcggc gtcgcaatag ggtcagggca atcactccat cgcagcgagt cggcatcagg
    19501 ttggttttcc cactcttccc atacagggag taacaaaacc agccagcttc ccgctttcaa
    19561 cgttccgcta agtgccgcaa aggcagcggc atcaaagccg tggcgggcgt cgaataccgc
    19621 atgccggaac tcgcgcccaa gtaaagtttg tagcgccgag ggagaacagt ggttttcagc
    19681 atctggctgc ggcgaaatcc acagccagtc gccaggtaag gcatcacgca acttaagcgc
    19741 atgatcaaaa caccaaccct cttccccgct caacaccagc aagcggcgga tcccttcacg
    19801 tttcatttgc gctgttaatg tgtgaagcgc agtcagttca gccatccctg ccccggaagt
    19861 taaatgcttt taccaaaagt attgcattgt gccggatcgc cgctgtcgaa accacgttta
    19921 aaccagctgt agcgttgctg agaagtgcca tgagtgaaac tgtctggtac tactcgcccc
    19981 tgactttgct gttgtaaacg gtcatcgccg atggcctgcg ccgcgttcag cgcctcttcc
    20041 agatcgccgg tttccagaac accttgctgc tgcatactat gcccccagac accggcaaaa
    20101 cagtcggcct ggagttccat acgcacagat aagcggttca cttccgcctg cgtcgcgttt
    20161 tgttgcagtt gacgaacttt cggctcgatg cctaacagtt tctgcacatg atgaccgact
    20221 tcatgggcga taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt gtctttcatg
    20281 tcatcataga aggagagatc gatataaacc gtgccatccg ccgggcaata gaacggtccc
    20341 attatggact ggcccgcccc gcagccggtg cgcgtcattc cacggtacat gaccagcttc
    20401 ggttgctgat aggttttacc catcttctcg aattgctgtc cccaggtgtc ttccgtggtt
    20461 gccagaatca ccgaagtgaa ttttgcggct tcgtcttcat taggactaat tgaccgtgtt
    20521 gattgctgtt gggaaaccgg ctgtccggtc atcaacccgg ttaaatcaac gccatagtag
    20581 cctgcaacca gcacgactat cagtaaaatc agcccgcctt taccgcttgg caggcgaaaa
    20641 cctggaccgc ccattgacgg accaccagag ctgttgcgcc tgtcttcaac attgtcactt
    20701 tcacgtcgcc cttgccaacg catagatacc tcaacaatat attcattata gaaatgatcg
    20761 taggtggttc agaggaagat taccacagga aagcgggatg aaatggcgga ggaatgagcg
    20821 ttcaatactc atcttatgag agcggttgtc ttgccgtgcc agctgcacgg caagatgatg
    20881 aacagaaaaa tcagtccagc tgtacaccca ggcggcgggc gacggcttca taggcctcga
    20941 tcaggccacc gaggctctgg cggaaacggt ctttgtccat tttctccagc gtttctttgt
    21001 cccacaagcg gctgccgtcc ggggagaact catcgcccag taccacttca cctttgtaca
    21061 gaccaaactc cagcttgaag tcgaccagaa tgagaccagc atcatcgaac agttttttca
    21121 gcacgtcgtt cgctttgtag gtcagctctt tcatgcgtgc caggttctct ttgcttaccc
    21181 agccaaaggt ttcgcagtaa gattcgttga ccatcggatc gtgcatggcg tcgtttttca
    21241 ggaacagatc gaacagtggc gggttcagct caataccttc ttcgattcca agacgtttca
    21301 ccagagagcc agcagcacgg ttacgcacga cacactcaac cggcaccata tccagctttt
    21361 tcaccagaca ttcggtatcg gagagcagac gctccatttg agtcgggata cccgcttcag
    21421 ccagtttgct cataatgaag tagttgaact tgttgttcac catacctttg cgatcaaact
    21481 gctcaatgcg cgcgccatcc cctgctgacg tatcattgcg gaattcgagc accaacaggt
    21541 ccgggttttc cgtgctgtat acggttttcg ctttaccacg atacaactca gcttgctttt
    21601 gcatctttat cactcctggg tgtgaattaa cgttttaaaa tctattgctg tctggtgtgc
    21661 cggatgtttt gtcggatgcg gcgtgaacgc cttatccgac ctacgtccga caaacaaatt
    21721 tcgtgcgaat taccgctaaa atcgcgtttt tctgccgacg cacacgtttg cgtatcatat
    21781 cagaaaaaag ggccggatga ctccagccct gtatttttac ttgctaaacg cagcctggaa
    21841 gacagctacc agcgcgtcgt tctgactctg agtcagagta tgacctttcg gatcgatgaa
    21901 ctgtaggctg ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac cggatgccag
    21961 acctggatcg ctcgcgccca gttcgtgcca ttcgctgtca gacagcggct tgtaagttac
    22021 ggccatgttg ccctgcgaac gggtgctgtc ggtcactttc atgcccactt tttccagcgc
    22081 agctggcaga cgttgccaaa ccacattgaa cggcccgcgt acgaccagca ttggtaaacc
    22141 ggtgtcatca gctgcacttt gtacgtccat agtggtggag gcacgatttt gcgcagcgtt
    22201 cgcggcgtca gtggcagatt tatccagacc ggcggaaata acgttcatca tctccgtgct
    22261 gtaacgctgc atggaagccg cgtctgcaac cggtttgccc gcctgttcca ggttcagcag
    22321 tttaaccgta accgcctgct gataaccctg cggcttaaca gagatttgat aacgaccacg
    22381 atactgctcg tcttcgtcca gacggttcca ttgtacccaa tcggtggtca gtgtctgacc
    22441 agcatcatca cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa ccacctgcgg
    22501 ccacagagta ttgccacgac cattttccac cagcaatgaa gcggtatcgc ccgtgaactg
    22561 ggtacgcgcg ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt ccagcgcctt
    22621 accgacagca ccactaccgt tggtcaccgg gattgcataa tcgccggagg tcaccggcaa
    22681 aatcattcca gccggggcat gaagctccgc aagcggtgcc gcttccaggt aggcttcatc
    22741 accactgacc tgacgcttat agcgtgagtc agaactacag gcagcgagta ataaaacaag
    22801 cgaaacaccc gcaacctttg ccaggcgcga cttttgaaca gagtaagcca tcaaatctcc
    22861 ctaaacttta cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca cgaccactgt
    22921 cggtgattgg tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc agttccttac
    22981 atgcccattt caccgggatt ggattgggtt cgacaaatag tttgttgtgt aatggcatca
    23041 gacgctgatt aataacgcgt gcctcggcaa aatgcccttc tgctgccagt ttgcacatct
    23101 gggccatatc acgcgctgcg acgttagccg taacggaaat aaccccatga ccgcctaatt
    23161 gcatgaagtc cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca tctgaaacca
    23221 gctctttgat ctggtttaca cgcgttaagt tccctgttgc ctctttgatt ccgataatat
    23281 tttttacttt cgccagacgg cccaccgttt ccgggagcag atcgcagcca gtacgggacg
    23341 gcacattata cagaatttgc ggcaggtcag tatgttcagc gatggctttg aaatgctgat
    23401 acaaaccttc ttgcgacgga cgattgtagt aaggggttac cgtcaggcag ccgacgatac
    23461 cgctgtcatt gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta gcaccggtcc
    23521 cggcaatcac cggaatgcgc ccgtcagcca gctccagcgt catcatcacc acatcagcat
    23581 gttcgtcatg atttaaggta gcggactcgc cagtggtgcc aacagaaacg atcgccgaag
    23641 taccgctggc gacatgataa tcaatcagtt ttttcatgct agcccgacag acattacctt
    23701 tttcatccat cggagtaaca atcgcgacaa tacttcccgt gaacatgggc catcctctgt
    23761 gcaaacaagt gtctcaatgg tacgtttggt atggcattaa aagcaagcag acagaaccgt
    23821 tctgattgtt gtatgcatgt tttttttatg ctttccttaa gaacaactca ccccttgaag
    23881 gaataaccag tttgacactg tcatcgcaac attatctggt gatcactgcg ttgggtgccg
    23941 atcgccctgg aattgtgaac accatcaccc gtcatgtcag tagttgcggc tgtaatattg
    24001 aagacagtcg cctggcgatg ctgggagaag agttcacgtt tatcatgctg ctttccggtt
    24061 catggaatgc cattactctg attgaatcaa cgttaccgtt gaaaggtgcc gagctggatc
    24121 ttttaatcgt gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca gcatctgtct
    24181 gggttcaggt cgatgtggca gactccccgc atttaattga acgcttcaca gcacttttcg
    24241 acgcgcatca tatgaacatt gcggagctgg tgtcgcgcac gcaacctgct gaaaatgaac
    24301 gggctgcgca gttgcatatt cagataaccg cccacagccc cgcatctgcg gacgcagcaa
    24361 atattgagca agcattcaaa gccctatgta cagaactcaa tgcacaaggc agtattaacg
    24421 tcgtcaatta ttcccaacat gatgaacagg atggagttaa gtaatgaatc cactgaaagc
    24481 cggtgatatc gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag ttaatttgac
    24541 cgacttccag ggacagcgtg ttctggttta tttctacccg aaagccatga cccccggctg
    24601 taccgtacag gcctgcggct tacgcgataa catggatgag ttgaaaaaag cgggcgttga
    24661 tgtgctgggt atcagcaccg ataaacccga aaaactctcc cgttttgcgg aaaaagagct
    24721 gcttaacttt acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat tcggcgtctg
    24781 gggtgaaaag tccttcatgg gcaaaaccta cgatggtatt catcgcatca gcttcctgat
    24841 tgacgctgat ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca atcaccacga
    24901 cgttgtgctg aactggctga aagaacacgc ctgattactt tgctccattc cgtgctggct
    24961 gcgcttgcgg ccagcacatc tcacatcttc tcactgccct gcaacactcg tttcaaattt
    25021 tttcgctgaa ggtcagtctt gcgcgatttg cccatcgggc caggcgtgaa tcacggcttt
    25081 gatcagcgtc gccaatggaa tggcgaagaa tacgccccag aatccccaca aaccaccgaa
    25141 gatcactact gataaaataa tgaccagcgg atgcaggtta accgcttcgg agaacaacac
    25201 aggtactaac aggttgccgt ccagcgcctg aatgatcaga tacaccgcga agcagctcca
    25261 gaattccgtg ccagcaccaa actggaatag cgccacgcca accaccggaa tggtcaccac
    25321 aaatgcacca atgtacggaa taagtaccga gaagccgacc agcaccgcca gcagcagcga
    25381 atagttcagc ccgaagagta agaatcccag ccaggtggca atccccacca cgatcatctc
    25441 cagcactttg ccgcggatat agttggtgat ttgttgattc atctccttcc acacctgtcc
    25501 tgccagtcca cggttacgcg gcagcacccg gcgaacggcg ttcagcatct gctctttgtc
    25561 tttcagcagg aagaagacca tcaatggtac cagcaccaga tagacggcaa tggtcagcaa
    25621 accgaccagc gaggcgaggg aaattttcac caccgaatcg cccatggtca gcatccgact
    25681 gcgcatattt tcggccattg catcaataat gcccgcatcc attaacgccg gatagcggcg
    25741 cggcaacgtg gcggcaaagt cagaaagctt attgagcatc cccggcatat cgcggattaa
    25801 gtagatgccc tgctgccagg cgataggcag taccacgaac gccatcagta gcaatatacc
    25861 gacgaaaacc accaatacaa tcgacgtcgc ccagcggcgg gagcagccaa tagattgcag
    25921 gcgcacggtt ggccattcca gcaaataggc cagcacaata gccaccaaca gcggagcaag
    25981 caggccacta aagaaaaaga taatgccaaa tccggcaact aaaataacca gcaaggcaat
    26041 cgcttccggg tcgctaaaac ggcggcgata ccattgcatc aacatttcga gcatacagac
    26101 cttccctgaa cctcaagagc gggattgcga tccgcaattg tatcgaaatg ccacaaaaaa
    26161 gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc atttgagtgg gctaatcttc
    26221 gccgttacac tcaaaggcgg cgcggtggga acgatatttc acagtatcgg tcaaatgact
    26281 atctccagaa atacaggata gaggttatgt tcaggcagtt gaaaaaaaac ctggttgcaa
    26341 ccctcattgc tgctatgacc attggtcagg tagccccggc gtttgccgac agcgcagaca
    26401 ccttgccgga tatgggaacc tccgcaggaa gcacactttc cattggtcag gaaatgcaga
    26461 tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc gttaattaat gatccgctgt
    26521 taacgcaata cattaattcg ctaggaatgc gtctggtttc gcatgccaat tcggttaaga
    26581 caccgtttca tttctttctg atcaacaacg atgaaattaa cgcctttgct ttctttggcg
    26641 gcaacgtggt gctgcactct gccctgttcc gttattccga taacgaaagt caactggctt
    26701 cagttatggc gcacgaaatc tcccacgtca cccaacgtca cctggcgcga gcgatggaag
    26761 atcagcagcg cagcgcgccg ctgacctggg tcggcgcgtt aggttctatt ttactggcga
    26821 tggccagtcc gcaggcgggg atggctgcgc tgaccggtac actggcggga acgcgccagg
    26881 ggatgatcag cttcacccag caaaatgaac aggaagcgga ccgcattggt attcaggtgc
    26941 tgcaacgctc aggattcgat ccgcaggcga tgccgacctt cctcgaaaaa ttactcgatc
    27001 aggcgcgtta ctcctcgcgt ccgccggaaa ttttattgac tcacccgttg ccggaaagtc
    27061 gtctggcaga tgcccgcaac cgtgctaatc agatgcgccc gatggtggtg cagtcgtcgg
    27121 aagatttcta tctggcgaaa gcgcgcacac tggggatgta taattccgga cgtaaccagc
    27181 tcaccagtga tttgctggat gaatgggcga aaggaaacgt tcgtcagcaa cgagcggcgc
    27241 aatatggtcg tgctttacag gcgatggaag ccaataaata cgacgaggcg cgtaaaacgc
    27301 tgcaaccgtt actggcggca gaacctggca acgcatggta tctcgatctg gctactgata
    27361 tcgatcttgg gcaaaacaaa gccaatgagg cgatcaatcg tctgaaaaat gcccgcgatt
    27421 tacgcaccaa tccggtgttg cagctcaacc tggcgaacgc ttatctgcaa ggcggtcaac
    27481 cacaagaagc ggccaatatt ctgaatcgct acacctttaa taataaagat gacagcaacg
    27541 gctgggattt gctggcacag gcggaagccg cgctaaataa ccgcgatcag gagctggctg
    27601 cgcgagcaga aggttatgcg ctcgccggac gactcgatca ggccatttcg ctgttgagta
    27661 gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc gcgttacgat gcgcgcatcg
    27721 accagttgcg ccagctgcag gaacgcttta agccttatac caagatgtaa ttcgcattaa
    27781 aggaagaaca atgaccaaac aggtaaaaat ctaccataac ccacgctgtt caaagagccg
    27841 ggaaacgctg aacctgctga aagaaaacgg cgtggagcca gaagtggtgc tctaccttga
    27901 gacacccgcc gatgcggcaa cgctgcgtga tttgctgaaa atgctgggga tgaatagcgc
    27961 tcgtgaattg atgcgccaga aagaggatct ttataaagaa ctgaacctgg ctgacagttc
    28021 gcttagcgaa gaggcgctga ttcaggcaat ggtggataat ccgaagctaa tggagcgccc
    28081 gattgtggtc gcgaatggca aagcgcggat tggtcgtcca ccggagcagg tactggaaat
    28141 cgtgggttaa gaagtgctcc atcgctagtt gaagcacatt gccggatgcg acgcttgccg
    28201 cgtcttatcc ggccgacgaa tactatgggt gcatatccga caataaacgt cttgtctaca
    28261 gtttcagaat ttctttcaca aacggaatgg tcagcttacg ttgcgcggta atcgacgcac
    28321 gatccaactg atccaacgtc ataaataacg tgcgcatctc tctgtcgagc cgcttcagca
    28381 agaaacgccc cacatcttcc ggcagttcaa aaccacgcaa acgcgcgcgt aactgtagcg
    28441 cctgcaactt atcttcatca gaaagtggct gcaatttgta gatctgcccc cagtcgagac
    28501 gcgacgcgag atccggtaat ccaagattca actgccgcgg tggacgatcg ccggtgatta
    28561 acaaccgtgt tttgcccgat tccagaattc gattgtagag atcgaaaatc gccatctccc
    28621 acaactcatc gcctgcaata cactcaatgt tgtcgataca gaccagcgac aaatgctcca
    28681 taccgtcgag cacttccgga acaaaccagg tgcgtttatc cagcgggaca tagcccaccg
    28741 catcgccacg ctgcgacaat tccgcgcaag ccgcgtgcag cagatggctg cgccccgcgc
    28801 cttcgcgtgc ccagagatag atgtaaccgc tatgttcctg acgcagcacg ttttgcagcg
    28861 cggccagtaa agaggagtta tcccccggcc agaaacttgc aaaggtttcg tcgtcaggaa
    28921 gataaagtgg caaagagagc tgtgccggtg tgttcagaga tacctcaacc aggatttcac
    28981 aaaatcgcga gaagtttacc acagaatccc atgatgtttg aaccgggcag caacactgcc
    29041 cggtcggtac attatttgtc tgttatatcc gcgtcttctg cgtccagcac cacttcttca
    29101 gggcgcagca tactaatcag tttgaagatc aaacttaagc cgataccgac gatggtcgcc
    29161 agcgccatac ctttcagctc cgccgcgcca atgtttacct tcgcgccact gacgccgatg
    29221 atcaaaataa cggaagtcag gatcaggttc tgtgctttgt tgtaatccac tttcgattcg
    29281 atcaacacac gaataccgga agcaccgatg acaccgtaaa gcagcagcga aacgccgccc
    29341 atcaccggca atggaatcat ctggatagct gcagccagtt taccgacgca ggaaagcagg
    29401 atagcgaaaa tcgccgcacc gccgataacc caggtactgt aaacacgggt gatcgccatc
    29461 acgccaatgt tttctccgta agtggtgttt ggcgtggagc caaagaagcc ggagatcacg
    29521 gtcgacaagc cgttagcaaa catcgaacgg tgcagacctg gatcgcgcag cagatctttt
    29581 ttgacgatat tagccgttac gaccaggtgc cctacgtgtt cggcaataac cactaacgcc
    29641 gctggtagaa tagtcagaat ggcaaaccac tcgaagcgcg gcgtatagag ggtcggcagc
    29701 gcaaaccagt gagcattaat aatcggcgtg gtatcgacaa ttcccattgc gaaagagagc
    29761 gcgtacccca ccagcacgcc aattaaaatc gggataattg ccaggaaacc acgaaacagc
    29821 acggaaccta aaaccgtgac cgccagggtg gtaatagaga tgatgatggt tttggagtct
    29881 ggcgtttgcc cttcagccgg gagtaaaccc gccataccgg cagctacgcc cgccagctcc
    29941 agaccgatga cggcaacgat tgcgcccatt gccgcaggtg gaaacagcac gtccagccag
    30001 ccggtccccg ctttcttcac gataaaagaa accaggcaga acagcacgcc gcacataata
    30061 aagccgccca gcgcaacttc ataccccaac ggcaacagta acaataccgg tgaaataaag
    30121 gcaaagctgg aaccaagata agccggaatt ttccctttac agatgaagag atacagcagt
    30181 gttcctatac cgttaaataa cagtacagtc gccgggttaa tatgaaataa gacgggcacc
    30241 aggacggttg caccaaacat ggcgaacaaa tgttgcaaac taagcgggat tgtctgtaaa
    30301 agtggcggtc tttcactcac cccgatagca cggcgcgtca tagtattatc ctctgtatta
    30361 tgtgttatag gcgctttact caaaaaaaag ccgactctta aagtcggctt taattatttt
    30421 tattctttat ttcgtaccaa agattttgtc accggcatcg ccgaggcccg gaataatgta
    30481 tccgtgctcg ttcagtccct ggtcaatcga tgcagtatac agttcgacgt ccgggtgcgc
    30541 tttttccagc gcagcgatac cttctggcgc agcgaccagc accagaactt tgatgctgct
    30601 gcagcccgct tttttcagca ggtcgatggt cgcgataacg gaaccaccgg ttgccagcat
    30661 tgggtcaacg atcagcgcca tacgctcatc gatgttagaa accagtttct ggaagtacgg
    30721 taccggctcc agcgtttctt cattacggta cataccgacg acactgatgc gcgcactcgg
    30781 aacgttttcc agcacaccgt ccatcatccc aagacccgca cgcagaattg gcacaacggt
    30841 aattttctta cctttgatct ggtcgatttc taccgggccg ttccagcctt cgatagtgac
    30901 tttttccgtt tcgaggtcgg cggtcgcttc gtaagtcagc aggctaccca cttcggaagc
    30961 gagttcgcga aagcgcttgg tgctgatatc ttgctcacgc atcagtccca gcttgtgttt
    31021 gacgagtggg tgtttgactt ccacgatctt catactcttt ctcctttgag gggcagccac
    31081 aaaaaaaatc gacggattat acctcctttc ttcaaggcgg caatattatt ttcgttgact
    31141 ttagtcaaaa tgataacggt ttgagataaa gttattttat attcaaatgg ttatggaaga
    31201 agattattcc atccgaaaac gatcctttgc cctagtacaa gtattctttc gccgcgcgcc
    31261 tggggaaaag acgtgcaaaa aggttgtgta aagcagtctc gcaaacgttt gctttccctg
    31321 ttagaattgc gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg
    31381 accgataaaa cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct
    31441 ctggttggaa gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt
    31501 ctgggcggct tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc tgtgctggtt
    31561 tccggcactg acggcgtagg taccaagctg cgtctggcga tggacttaaa acgtcacgac
    31621 accattggta ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcggaa
    31681 ccgctgtttt tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg
    31741 gtgatcagcg gcattgcgga aggttgtctg caatcgggct gttcactggt gggtggcgaa
    31801 acggcggaaa tgccggggat gtatcacggt gaagattacg atgtcgctgg tttctgcgtt
    31861 ggtgtggtag aaaaatcaga aatcatcgac ggatctaaag tcagcgacgg cgatgtgctg
    31921 attgcactcg gctccagcgg cccgcactcg aatggctatt cgctggtgcg caaaattctt
    31981 gaagtcagcg gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat
    32041 ctgctggcac cgacccgcat ttacgtgaag tcagtgctga aattgattga aaaggtcgat
    32101 gtgcatgcca ttgcgcacct gaccggcggc ggcttctggg aaaacattcc gcgcgtattg
    32161 ccagataata cccaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac
    32221 tggctgcaaa cggcaggtaa tgttgagcgc catgaaatgt atcgcacctt caactgcggc
    32281 gtcggtatga ttattgccct gcctgctccg gaagtggaca aagctctcgc cctgctcaat
    32341 gccaacggtg aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc
    32401 gtggttatcg aataatgaat attgtggtgc ttatttccgg caacggaagt aatttgcagg
    32461 caattattga cgcctgcaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca
    32521 ataaggccga cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc
    32581 tcatcgccag cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg
    32641 acatgtacgc acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt
    32701 ttgtctccca ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc
    32761 ccggattaca cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg
    32821 tgcatttcgt caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg
    32881 tatttgctgg tgatacggaa gatgacatca ccgcccgcgt gcaaacccag gaacacgcta
    32941 tttatccact ggtgattagc tggtttgccg atggtcgtct gaaaatgcac gaaaacgccg
    33001 cgtggctgga tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccg
    33061 tagttaaagc gccagctctg ccgctggcgt ttttcaattc acctgtaaat cgcaagctcc
    33121 agcagttttt ttcccccttt tctggcatag ttggacatct gccaatattg ctcgccataa
    33181 tatccaggca gtgtcccgtg aataaaacgg agtaaaagtg gtaatgggtc aggaaaagct
    33241 atacatcgaa aaagagctca gttggttatc gttcaatgaa cgcgtgcttc aggaagcggc
    33301 ggacaaatct aacccgctga ttgaaaggat gcgtttcctg gggatctatt ccaataacct
    33361 tgatgagttc tataaagtcc gcttcgctga actgaagcga cgcatcatta ttagcgaaga
    33421 acaaggttcc aactctcatt cccgccattt actgggcaaa attcagtccc gggtgctgaa
    33481 agccgatcag gaattcgacg gcctctacaa cgagctgttg ctggagatgg cgcgcaacca
    33541 gatcttcctg attaatgaac gccagctctc cgtcaatcaa caaaactggc tgcgtcatta
    33601 ttttaagcag tatctgcgtc agcacattac gccgatttta atcaatcctg acactgactt
    33661 agtgcagttc ctgaaagatg attacaccta tctggcggtg gaaattatcc gtggcgatac
    33721 catccgttac gcgctgctgg agatcccatc agataaagtg ccgcgctttg tgaatttacc
    33781 gccagaagcg ccacgtcgac gcaagccgat gattcttctg gataacattc tgcgttactg
    33841 cctcgatgat attttcaaag gcttctttga ttatgacgcg ctgaatgcct attcaatgaa
    33901 gatgacccgc gatgccgaat acgatttagt gcatgagatg gaagccagcc tgatggagtt
    33961 gatgtcttcc agtctcaagc agcgtttaac tgctgagccg gtgcgttttg tttatcagcg
    34021 cgatatgccc aatgcgctgg ttgaagtgtt acgcgaaaaa ctgactattt cccgctacga
    34081 ctccatcgtc cccggtggtc gttatcataa ttttaaagac tttattaatt tccccaatgt
    34141 cggcaaagcc aatctggtga acaaaccact gccgcgttta cgccacattt ggtttgataa
    34201 agcccagttc cgcaatggtt ttgatgctat tcgcgaacgc gatgtgttgc tctattatcc
    34261 ttatcatacc tttgagcatg tactggaact gctgcgtcag gcttcgttcg acccgagcgt
    34321 actggcgatt aaaattaaca tttaccgcgt ggcgaaagat tcacgcatca tcgactcgat
    34381 gatccacgcc gcacacaacg gtaagaaagt caccgtggtg gttgagttac aggcgcgttt
    34441 cgacgaagaa gccaacattc actgggcgaa gcgcctgacc gaagcaggcg tgcacgttat
    34501 cttctctgcg ccggggctga aaattcacgc caaactgttc ctgatttcac gtaaagaaaa
    34561 cggtgaagtg gtgcgttacg cacacatcgg gaccgggaac tttaacgaaa aaaccgcgcg
    34621 tctttatact gactattcgt tgctgaccgc cgatgcgcgc ataaccaacg aagtacggcg
    34681 ggtgttcaac tttattgaaa acccataccg tccggtgaca tttgattatt taatggtgtc
    34741 gccgcaaaac tcccgccgcc tgttgtatga aatggtggac cgcgaaatcg ccaacgcgca
    34801 gcaagggctg cccagcggta tcaccctgaa gctaaataac cttgtcgata aaggcctggt
    34861 tgatcgtctg tatgcggcct ccagctccgg cgtaccggtt aatctgctgg ttcgcggcat
    34921 gtgttcgctg atccccaatc tggaaggcat tagcgacaac attcgtgcca tcagtattgt
    34981 tgaccgttac cttgaacatg accgggttta tatttttgaa aatggcggcg ataaaaaggt
    35041 ctacctttct tccgccgact ggatgacgcg caatattgat tatcgtattg aagtggcgac
    35101 accgctgctc gatccgcgcc tgaagcagcg ggtgctggac atcatcgaca tattgttcag
    35161 cgatacggtc aaagcacgtt atatcgataa agaactcagt aatcgctacg ttccccgcgg
    35221 caatcgccgt aaagtacggg cgcagttggc gatttacgac tacatcaaat cactcgaaca
    35281 acctgaataa ccctatgcca atacacgata aatcccctcg tccgcaggag tttgctgcgg
    35341 tcgatcttgg ttcaaacagt tttcacatgg tcatagcccg tgtggtagat ggtgccatgc
    35401 agattattgg ccgcctgaaa cagcgagtgc atctggcgga cggcctgggg ccagataata
    35461 tgttgagtga agaggcaatg acgcgcggtt taaactgtct gtcgctgttt gccgaacggc
    35521 tgcaagggtt ttctcctgcc agcgtctgta tagttggcac ccatacactg cgtcaggcgc
    35581 tgaacgccac tgactttctg aaacgcgcgg aaaaggtcat tccctacccg attgaaatta
    35641 tttccggtaa tgaagaagcc cgtctgattt ttatgggcgt ggaacatacc caaccggaga
    35701 aaggtcgcaa actggttatt gatattggcg gcggatctac ggaactggtg attggtgaaa
    35761 atttcgaacc gattctcgtt gaaagccgcc ggatgggttg tgtcagcttt gcccagcttt
    35821 atttccctgg cggggtcatc aataaagaga attttcagcg cgctcgcatg gcggcagcac
    35881 aaaaactgga aactttaacc tggcaatttc gaattcaggg ctggaatgtt gcaatgggcg
    35941 cttccggtac aataaaagcc gcccatgaag tgttaatgga aatgggcgag aaagacggga
    36001 taattacccc ggaacgtctg gaaaaactgg taaaagaagt tttacggcac cgtaatttcg
    36061 catcgctgag cttaccaggt ctttccgaag aacggaaaac agtcttcgtt cctggactgg
    36121 cgattttatg cggtgtgttt gatgctttag ccatccgtga actgcgcctt tctgacgggg
    36181 cgttacgcga aggcgtactg tatgaaatgg aaggacgttt ccgtcatcag gatgtgcgta
    36241 gtcgcaccgc cagcagcctc gccaaccagt atcacatcga cagcgaacag gcccgacggg
    36301 tgctggatac cactatgcaa atgtacgaac agtggcggga acagcaaccg aagctggcgc
    36361 atccgcaact ggaagcgcta ctgcgatggg ccgccatgct gcatgaggtc gggttgaata
    36421 tcaaccacag cggtttgcat cgccactccg cttatattct gcaaaacagc gacttgccgg
    36481 gttttaatca ggaacagcag ttgatgatgg cgacgctggt gcgctatcac cgtaaagcga
    36541 ttaagctcga cgatctgccg cgctttacct tgtttaagaa gaaacagttc ctgccactga
    36601 ttcagctatt gcgccttggc gtattactca ataatcaacg tcaggcaacc accacaccgc
    36661 caacattgac gctgattacc gatgacagtc actggacact gcgttttccg catgactggt
    36721 ttagtcagaa tgcgctggta ctgcttgatc tggaaaagga gcaagaatac tgggaaggcg
    36781 tggctggctg gcggttgaaa attgaagaag aaagtacacc agaaatcgcc gcttaatgct
    36841 ttgcgggccg acgagaaatg tcggcccgca ttattcaggc actttcgcga atgggttcga
    36901 tttcattcag cgtatcaatt aacggttgcg gcttaccaat aagataaccc tgcatataat
    36961 cgatccccaa agagagcacc gcctcgcgga tctcttcgtt ttcaacgtac tctgccacta
    37021 cccgcatttt cttcattcgc gccaggtggc aaatcgacgc cactatctga taatccagac
    37081 tatttgacac aatattgcgg ataaaactgc cgtcaatttt aagcagatcg gcattcacat
    37141 ttttaagccg cgcatagctg gcgtagccgg tgccgaaatc atcaatcgca atctggcagc
    37201 ctaattcctg aagatgctgc aaggtgattt gcgcctgctt aacattggtc agagcattac
    37261 tttcggtgac ttcaaaaata agttgccacg cttcaatctg atatttagcc agcagctgac
    37321 tgatttcaac aggaaaacga gcctgacata ccgaggttgg cgacagatta atcgcaaaac
    37381 ggtgagcggg catcttcgct ctgttttcag ccataaattg cagcgtatgc tcaatgaccc
    37441 acatgtcgat actcgacgat aaaccaaatt cgtgcgcgac cggtaagaag ctatcggggc
    37501 tgatcagttc atcattctca cctttcatgc gcagaagaat ttcatggtag acatcaccac
    37561 gcataccggt aatcggctgg gccatcagga aaaaatggtt gtgttccagc gcccgctgta
    37621 gccgattcat catcgcgact ttatctttca attcgcgttg caaatacatt gctccgcgac
    37681 gctgcatatt ttccggggcg ttggtcacga tagaaagttc ggccactgta tttagctctc
    37741 ccagcagcag gtagatatga ttcactggcg agcgcacata gcagtaactg acgccaatct
    37801 gcggttgcat cggcattcca tcccaaaaga aacgaaattg cttgagatgg ctatccagtg
    37861 cggtaatgcg ctcctggtgc gattctgtat tcagccgcag cgcgagatcg ttacccgaaa
    37921 gctgataaac atcttcaccc ggttccagca atggtgacag ccagtgagaa agtttttgct
    37981 tgtattgaat ccgcagcatg atgccataat tcttaaccag catttccatg ccagggatgc
    38041 gtaaataaca aagcgcagac cagggggcat cacgtaacgc gcgattcagg gcgcgaacat
    38101 ttggcagatg aaccaccggg tccacatacg caagccgctg aatgcgtctg actaccgctc
    38161 gctgacgggt tgccagtaca gccatataat tgacaataaa agagaatacc agataactgg
    38221 aggaggttat ggtcagctgc gtggtatagc caggataaat gggaatgtaa ttttgatagc
    38281 tgtggatgct gatcatcaac acgaccgccc agagcaatga aatcagctta taaccatagc
    38341 gcatcgctcc ccacatcatc aggggcagca ataatgacaa ggtgtaattg gtgctaaaaa
    38401 tcgtgctttt ttcatttaac ggcatgcata acagcaatag taaagcacct aatgccagta
    38461 gccagattgc gaactctttt ttggtaactt tggcatcaac ctgctgtttt aattgcgaat
    38521 aatagctacg taaataaaac ggatttcgca ccacccgaat gatgaagtag cacagcggga
    38581 caccaatcag attacccacc agcaaggcct gataattgat taaggtcccg aggttaaagg
    38641 gcatgacgcc gaccagattt tctctgctcg ccagtaatcc aacaaacgca gcaaactgga
    38701 aaagtatcag aaacagcgtt gcaggaaaca caatctgcca gaatagtcgc tgggaaatta
    38761 aacggctatc accatgtgaa acgttgttac gccggggagt aaaaaccctg taaccgcccc
    38821 aacagagaat gataatgata ataaaattag ccgttaatga gagcgtttcg taaaaaccca
    38881 gctctgcata tttgcggaca aaaatcccta aggcgatccc tggcaatgcc gcccagctga
    38941 aaaacatcat catgctgatc atcagtgcca gtggcagata aaagagaaaa acttcaccgg
    39001 aagaaatatg cgcgaaagta ttaatgtggg cgaaaatggg caagattaaa gaaggcagga
    39061 acagcggaag cccccaccat ttatcacgta tttttatata agttgcattc agtttcatag
    39121 atgctcagca gaatccccca catcctgaag gaggtgtatt cagacagaca tcccacctga
    39181 cttcgaatga tgattattca tcactataga gagcaatgat tctaagtgtc atatgaaagt
    39241 tccaattgat atatatcaaa caaaataacc ctgattaatg aattattacg tttatcatgt
    39301 taactcatca ttattacata atcattgtaa ataattaaat taacttccat aacattaaaa
    39361 tatgtatcca ctgacgcttt tttacataac gaagaattga ccattttgtc ctgttgtgcc
    39421 ttaatgtaag taccgtccac ggcgtgggac atacttcaag gaaccttttg tgagtcaggc
    39481 aaccagtatg cgaaaacgac accgatttaa cagtcgcatg acccgtatcg tactgctcat
    39541 cagctttatc ttcttctttg gccgttttat ctactcgtcc gtcggtgcct ggcagcacca
    39601 tcagagcaaa aaagaagctc agcaatccac actctccgtc gaatcaccag tacaacgtta
    39661 gcggttacct tctccactct cacagaacat aacggcacct cgctgtcgga tgcttttgct
    39721 ctttgggatt atcaaagcgg cagatatttt ttcatcttaa attttacgtt tttgtcctga
    39781 ctgatgttta tcctgttcga ctgcgaaata aatacatcat taatacatat gttgtgatga
    39841 tgtattttta taaattattt cccgcatgaa ttttaataaa tttaatctat tcctttatac
    39901 gcaatacatt tactttcctc ttttgatgat cttaaatgtc ttatttttcg taatgtgtat
    39961 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
    40021 tcactctggc aggatgccag tcaaatgctg atgatcatgc cgccgatgtt tatcaaaccg
    40081 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
    40141 aagttgccgt agacaacgcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
    40201 gcgctgtcgc tggcggtgtt atcggccaca acgtcgggtc tggcagcaat tccggaacga
    40261 cggcaggtgc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
    40321 aaaccttagt ggaaggtgtt tctttaacat ataaggaagg caccaaagtg tatacctcca
    40381 cccaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacgacgt
    40441 ataacgaaac acgtattcag ccaaatacca aatgtcctga aaagagctaa taatcaggag
    40501 gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat ccggctatcg
    40561 cctcatcatt gcaggatcaa ctctcggctg tcgcagaagc ggaacagcaa ggtaaaaatg
    40621 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
    40681 agcaacgtcg cgcaaacgcc caggccgcgg ctaataaaag agcggcaacg gcagcggcga
    40741 ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa aaacgcgatc
    40801 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg gcgctggcga
    40861 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggaactg aagcacaaag
    40921 ctgcgcaaac cgatgtggtg caatctgaag ctgacgcaaa caggaatatg actgaaggcg
    40981 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa taaatcagat agctggttta
    41041 actaaacgat gttagtaact tcaaacctat aattctttag gataaaaaac cctctgtagt
    41101 aacagagggt tttgttcatt catagtgcag ggtcaaatca ttcccactca atagttgctg
    41161 gtggcttgcc gctgatgtca tacaccacgc gggaaatacc attcacttca ttgataatgc
    41221 ggttggaaac gcgaccgagg aaatcgtacg gcagatgtgc ccagtgtgcg gtcataaagt
    41281 cgatggtttc gacagcacgc agagagacaa cccagtcata cttacgacca tcgcccatta
    41341 cgccaacgga acgtactggc aggaagacgg tgaatgcctg gctgactttg tcgtacaggt
    41401 ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacaatact
    41461 ctttcttcac ttctcccaga acacgaacgc caaggcctgg tcccgggaac gggtgacggt
    41521 acaacatgtc atacggtagg cccagctcca gaccaatctt acgcacttcg tctttgaaca
    41581 gctctttcag cggttcaacc aagcccatct tcatctcttt cggcaggcca cccacgttgt
    41641 ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc agcagattcg ataacgtcag
    41701 ggtagatagt gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga
    41761 atacttcaac gaaaacgcgc ccgatgattt tacgttttgc ttccggatca ttttcgccag
    41821 ccagcgctga caagaagcga tcttctgccg gtacgtgaac aatattcaga ccaaagtgat
    41881 cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga
    41941 atacgcaagt caggttttta ccgatagcgc ggtgcaacag cattgcggtt acggaggaat
    42001 ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag
    42061 ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac
    42121 gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact
    42181 gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc
    42241 tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga
    42301 catcgagcag cggtttaccg tctgcggtca gagcatcttc aataccgcga accagtgcgc
    42361 tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt
    42421 gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc
    42481 ctgcttcaaa aacatactgc ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc
    42541 cgccggaaag aataatgccg cttgggttga agtcacgaat ttgtgcttct gtcacatccc
    42601 acgcccacag ttcgcagtaa acacccagtt cacgcacgcg gcgcgcaacc agttgagtgt
    42661 actgggaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg
    42721 cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga
    42781 gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg
    42841 gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc
    42901 gatagtacca cagccggtca gccccataca ggagcgcagg ccgcccatct gctggtgaat
    42961 gatctctttc aggcgacctt tataggccac gcgaccttcg ataccttccg gcaccagttt
    43021 gtcggcagcg ttgtcgctct ggaagtaacg gtcagaagaa cctttggaca tcgcgcccag
    43081 ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg
    43141 agattcttca gtacctgcca gcatggaacc caccatcacc gcgcttgcgc ctgcggcgat
    43201 agctttggcg atatcgcctg agaaacggat accgccgtca gcgataaccg gaatgccagt
    43261 accttccagg gcttctactg catcagcaac ggcggtgatc tgcggaacac cgacaccagt
    43321 cacgatacgc gtcgtacaga tagaaccagg gccgataccc actttaacgg cactgcaacc
    43381 ggcttctgcc agcgcgcggg cacctgcagc tgttgccacg ttgccgccga tgatttgcag
    43441 gtcaggatat ttagcgcgag tttcacggat acgctgcaga acaccttcag agtgaccgtg
    43501 agaggagtcg atcagcagaa cgtcaacgcc tgcggcaacc agcgcatcaa cacgctcttc
    43561 gttaccagcg ccagcaccaa ccgctgcacc aacacgcaga cggccttgct cgtctttaca
    43621 ggcgttcggt ttacgttctg ctttctggaa atctttcacg gtgatcatgc cgatcaggtg
    43681 gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac
    43741 cacttcacgg gcttcgcctt cacgcacggt taccagacgc tcttttggcg tcatgtaaac
    43801 gctaaccggt tgattcagat cggtcacaaa acgcacgtca cgaccggtga tgatgccgac
    43861 cagttcgttt tcttcggtaa cgaccgggta gcccgcaaag ccgttacgct cggtcagttc
    43921 tttcacttcg cgcagcgtcg tggttggcag aacagtctgc gggtcagtca ccacaccaga
    43981 ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg
    44041 gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt
    44101 atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct
    44161 gaggtcagca gtattcggca gaacggtaga atgtgcagga acgaggagaa cgtcgtcaaa
    44221 cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa
    44281 atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat
    44341 gcttgctatc cccggagggc gggttactat cgactgaata acctgctgat ttagaatttg
    44401 atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagc cgcctgaatc
    44461 aaacggttcg tctgctgctt gagcatgaga tggggcaggt gtggatcagc ggcgaaatct
    44521 ctaatttcac acaaccggct tccggtcact ggtactttac gcttaaagac gataacgccc
    44581 aggtgcgctg cgcgatgttc cgcaacagca atcgccgggt caccttccgc ccgcagcatg
    44641 ggcaacaggt tttagttcgc gccaacatta cgctgtatga accgcgcggc gattatcaaa
    44701 ttattgtcga aagtatgcag cccgccggtg aaggtttact gcaacagaag tacgaacagc
    44761 ttaaagcgaa gttgcaggct gaaggtttgt tcgatcagca acttaaaaaa ccactcccct
    44821 cccctgcgca ctgcgttggg gtgattacct caaaaacagg tgccgcactg cacgatattt
    44881 tgcatgtgtt gaaacgccgc gatccgtctc tgccagtgat aatctatccc actgccgttc
    44941 agggcgatga cgcaccaggg caaatcgttc gcgccattga gctggcgaat cttcgcaacg
    45001 agtgcgacgt attaatcgtc gggcgtggcg gtggctcgct ggaagattta tggagtttta
    45061 atgatgagcg tgtggcgcgg gcgatttttg ccagtcgcat tccgatagtc agcgccgtcg
    45121 ggcatgaaac cgatgtgaca attgctgact ttgttgctga tctacgtgcg ccaacgccgt
    45181 cagccgccgc tgaagtggtg agccgaaatc agcaagaatt gctacgtcag gtgcaatcga
    45241 cccatcaacg gctggaaatg gcgatggatt attatctcgc caaccgcacg cgtcgtttta
    45301 cgcagattca tcatcgcttg cagcaacagc atccgcagct ccggctggca cgtcagcaaa
    45361 ctatgctcga acgtctgcaa aagcggatga gctttgcgct ggaaaatcag cttaagcgtg
    45421 ccgggcaaca gcagcagcga ttaacacaga ggctaaacca gcaaaatccg cagccgcgta
    45481 ttcaccgcgc acagacgcga attcaacaac tggaatatcg tttagcagaa accctgcgcg
    45541 cacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacacacctc gaagccgtaa
    45601 gcccactgtc aacgctggcg cgcggttata gcgtcaccag cgccgctgat ggcgcggtgt
    45661 taaaacaggt taagcaagtg aaagtgggtg agacactgac cacacgactg ggagatggtg
    45721 tggtgattag tgaagtcagc gccgtaacaa aaacccgcaa atcacgtaaa aaaacgtcta
    45781 atccgtagac cggataagag gcgccagcat cgcatccggc aataactccc aggccacgcc
    45841 agatccgcca tgctggttac tggaagcagg tcaccagacc cggtttattt attagcgtga
    45901 tactacaatc ctgtgctccg gtaccttcgt ccatcccaca ttgttgagag gacaaataag
    45961 gaaaactggg tgaagacggc catcccagtg aattaaaaac agtatgcaac aactctaatt
    46021 cattgtatgt cggtgcattt tctatgctgc cacactgcct tgcgatatcc catttactgg
    46081 catcaatgat atgtgcagaa gcccagtttt cgtagttaat aatcggtgag gatacgggat
    46141 tagccccgga cggcaattct gtcgttaaca acggtcgatg tagcgattta ccattcacca
    46201 ctaccgtgtc tgccatatgt ccccaaaagt gcgccgagga aacatttggg ctggttggta
    46261 ccgtaaaaat agcgtccagc gtgtctgtcg cggaagcgta attagccaga gtaaccgtca
    46321 gtggcgtttt ataaccaggg gtattatcct ggcggagcgt aaaacttgcc gtcccatccg
    46381 cgcctgtcat gccactaatg gtattaccgc tttcactcag cgtaacagcc gaaccagata
    46441 acggctgaac gatgagatcg tccatcgcca ccacatcacc atacaacgtt gccccagcgt
    46501 tacgcggcga tgcctcaccg cgtttcaaag taaattccac attcggcacc gtattcccgg
    46561 cgctatcttt caccgtcacg gtaacgggca tggcttcgcc ttttttcact ttcgccgcct
    46621 gagtggctgc atcaaacgct gttgaggtaa gtgtgacgct ggaaaccgtt gaacgcgcgt
    46681 tagccaggca aggctgaaat gattttgttg aacccgtata cagcgtgcca tttctcaaat
    46741 caaccgactg gtctttacca tcggcggatt tatcctctgc ccaccaagtg taatacgcag
    46801 gccagccagt ttcagtgaca aacgtgttat tcggataacg cgcagacaac gaacttaaat
    46861 cattgaatag tggctgcctt gctacttcac aagggctcac actcgcatct gatttctgtg
    46921 ttggggtaaa gtacggccag tcttcattat tgttattgat cttgccgacg ccagaagtcg
    46981 atgtcagttc actccacaat ttcgggcgtt caaataccgc accattcact gccgtcaagg
    47041 ttgctggcat atgcccccag tagttagcct tcgcagaatc cgggctggtc actaccgtga
    47101 atatcaggtc ctgattagcc gtaacctgcg gatttcgcgc caatgaagct gtaaacggcg
    47161 ttaccagacc gacggtgtta ttttgggtaa cgttgaacgt tatcgtgcca tcactgccgg
    47221 tgaagccgct ccatgccgac gtattctggt tgaaggagat acgcgccgat gtcggggtga
    47281 cttcatctaa aatgagatca tctacggtat tggaggatac agagatccct gaacggcttt
    47341 tcgcctgcac gcgcttgagc gtaacacctt cgccgccaac aggattacca ttggcatctt
    47401 tgatcgtcac cacgatggga agcatctcac cttttttcac agatgacgtg ggggtaccac
    47461 cattgctggc agttcggccc tcatcatagg cagtcgatga cagcgtaagg cttgctggtg
    47521 ccgaatgtgg ctgcgtcaga cagagcatta aagcgtatcc attaatagtc gtcgtcgatt
    47581 gtcccgtttt caaattaatg acgccgtaat accacgtcaa cgcgtcagtg gtgctcattt
    47641 cataagtcca ccaatttcca cttccggcag ttataggcaa ccccaaatct gtcagtattg
    47701 tcccgcctgg atgatcatta tatagcccct gtaactcatt gagacgcggc tgatactcaa
    47761 ccgggcaatc cggcgtaact ttattggtat aaaaaaggta tgaccacgac tcattattat
    47821 tacttaccga ggaccccaag gtcgcttctg ctgccagtag cggtcgttta aaggtcaccc
    47881 ccgccctact ggtaaacgtg tcgggcatat gaccccagta ttttgctttc ggtgagttcg
    47941 gactggttat cacagtgaaa ataacatcca tcgtggcact ttttgtggtg tcgcgcatca
    48001 atgttgcagt gagcggagtt gccagcccat aagtctcgtt ttgcgtcaca tcaaaagtcg
    48061 ctttaccatc acttcctgtc gtcagaagtg cctgtgcccc attattatag agtgacatgt
    48121 ttactccggc aggggtgacc ggcacgatag taagatcatc atacgtagaa tctagcctgt
    48181 tttttgcacg atccaacgca gtcccgcgta tgagattaaa gtttgcgcct ggcaacagat
    48241 ttcctgcgct atcccgcaca atcaccgtca tccggatcgc cgtacctttc gccgccgttg
    48301 cactagggcg accatcgttg ctggccgttt tctccgcatc cagtgccgtg gaggtcatct
    48361 ctatagaagc tgccactgca tgagggttta ccagacagga cacaaatgcc acggtgtttg
    48421 ataatgccct ggagcttgat ccattcgata aattaataga ctgatcgtac cagacgttcg
    48481 cgtaggcaat tttatcgtaa gcccaccagg tgttagtcag cggaaggcct aaatcggtcc
    48541 ctatcgcacc attaggatgt tcactgtata actcttgaaa ttcgcttaat aacggttgat
    48601 aaggttcgcc gcagtctgat ttgctgacat cattttttgc tgccgtattc aaagaaagcc
    48661 atgattcatt attagccatg gttgactgcc ccgctggatg ctcggcagta agcaatggcc
    48721 gcttaaaggc aattcccgac cgggtagtaa acgtttcagg catatgcccc cagtaacttg
    48781 ccagtggcgt atccgggctg gttatgacgg taaacatgac cggtaatttg ttggattcta
    48841 tcgtcggctg atacaactgg gcgtacatat tggttaccag gcccatagaa tcatcttgtt
    48901 ttaccgtaaa ggaggtagtc ccatccgcgc cagtcacccc ataaagcagg cttgaagaac
    48961 tattcatcct cgcagaagcg cctgccgcgt taattaccgt aatcgactta tttatcatct
    49021 cggcattttg acggttcttc gagccttcac gtctgagaat aaaggggaca ttgggcacgc
    49081 tatttccctg cgcattttta gtcaccacgc gaatcaccgc gtcttcgcct tttttcactt
    49141 tcaacgcact caaggtgtcg tcaactttga caaattgcgc cgtatcattc gcctcaacga
    49201 tgatctgact ggcaacggcg acaggcgtgg tccggcagct cagtaatgca ccaccagatg
    49261 agggcatctg cgtttcatct cctgtcttca gcgaaacgac atcatacgta aaggtttgag
    49321 tgaacgtatc tgcaacggta ttcgagatat agaacctcga cactggccag ccaatagcat
    49381 catgtaatgc gctactcccc tggtgcgcat aaatggccct aagttcattg tcagttggaa
    49441 catactccgc gccacaacca tttatcgagg tatcgttagc ctggcccgac gggaacgtag
    49501 accaggcctc cccctcttcc accagggtac cgatactggt ggtagcacta ttctcgtccc
    49561 tcagtaaagg ccttcgataa ataagaccgt catcgcctgt cgccgtttca gccatatgcc
    49621 cccagaattt ggcttttggc gaatccgggc tggtaagcac cgtaaagaca acgggaagcg
    49681 tcgatttagt gctgctggag gaatccaacg ctgccgtgag ttctgttttt agcccggtag
    49741 tctggtcttg cttcaatgtg aaagtggtga tgccatcagc ccccgtcacg ccataaataa
    49801 ccagggtcgt tgaaagaaaa tcatcttgag tatttcccca ggcatccgtt accgccagcg
    49861 aagcaatcga cgtcgtagtt gacacattgg ccctgttcac gctggtgttt cgtttgaggg
    49921 taaatgcggt atttcccaca gggttaccct gcgcatcttt agtggtcact cgtaatacgg
    49981 cttcttcgcc ttttttcgct ttgacaacat tattcgtact gtcaaatttt gtggggtctg
    50041 cagcttcaag aacaatttgg cttgccttaa caatcggcgt cgtctggcag gtaagcagtt
    50101 cttttgtggc agatggaatt atcgaactgg tgcctgaact gagattgacg gccttgaagt
    50161 cacgctgttc caggctacta ctacctgtcg tgctggaaag gtaacttgag gcgacgggcc
    50221 agccctgaac cgttttcatc gcattgccgc ggttggcttc atacaatgac tccagcccgg
    50281 cctgggaggg aacatattca ctaccacaac cattagagga tgtgcttata gcctgggttt
    50341 gggtaaattg cgcccagtct tcattctctt ctgcaatcgc tgttcgaccc gacgtactgg
    50401 ataactcttt caacaataat gggcgtttaa agaccgttcc atctgcagcc gtcactgttt
    50461 caggcatatg cccccacatt ttcgccttat cgctgtccgg gctggtgaca acggtaaaaa
    50521 tggtatcgag ggtcgctttt ttcgttgtgt ctgaatagag cgcggcggtc agcgacgttt
    50581 ttgtgccatg ggtatccggg cgggtaatag tgagaatttt agtgccatcg ctgccggtca
    50641 tcgcgctgta tgctgcggcg gtatcattga gcgaagtttc atccgccagg ccactattga
    50701 tcacaaccgg cgcaaccagc gcatcaccgc caccggcaat gtgtttttcg tttgatcggg
    50761 tataaccatc gccacgcttc agcgtaaaag gcatatcacc caatggattg ccctgagcat
    50821 cttttaccgt caccttcacc tgtagcgttt cgcctttttt cagcttcgcc gcatttgctg
    50881 ccgcgttcca ctgcgccggg tccaccactt ccagcgtaat gctgctggca ggattgttag
    50941 ccgtcgtcag gcaactgaca taaaccgccg tgctaccgcc aacggtccgc gcgccatcgt
    51001 tgagggctat ggtgtagtaa tgcggcacct gatccgcggg cgaactgctc cagtagggct
    51061 gacgctgcgt tggccaaccg tgtaccgttt gaacggcgtt accgctgtta gcatcataga
    51121 gcgcgttaag ctgggaacgg cgtgggagca tattggcacc acagccacct ttactggtgc
    51181 tggaagtgtt ggcctggcta acgcgcgacc acacttcgtt attttccgtt aatgtgccgt
    51241 ccgggctgga cacttccgca gccagccggg gtcgggcaaa ggttaatgca tcgacggtaa
    51301 tcgtctcatc catatgaccc cacatctgcg cgccaaccgc atccggactg gtgagcgtag
    51361 tgaaaatcac attgtaatta acagtattgg gaatatagga gtttacaggc gcaatattca
    51421 gcggcgtcgc taagcccaca ccttgcggtt gcttaatttc cacagtagca aaaccctgcg
    51481 catccgtagt accggcatag actaaagacg gttgcgatgt tccgtagagt gtgccattca
    51541 tctcgattgc gccgcgggtt ggatcatcaa aacctgtagt ctgcccctga cgatttttcc
    51601 ccatatcttt agtgatggtg aacgcggtat acggcacggg cgcattattc aaggcattaa
    51661 aggtgcgtac cgtcatggta atcgtttcac ccactttagc ctgcgctctg ctgcttgtgg
    51721 aaacatcatg atcggtggtc gcagcaatct gggcgaccat ctcattgccc gaacaagaaa
    51781 ggtagttttg cttaaagccg gaataggtgt cgatgttgcc cgtggccagg ttcaccgacg
    51841 cgtgcgacgt ctcttccacc gcactaagat acgctttttg caacgttggc cagccatatt
    51901 cggtgccgat gacattggct ggatgtgcgg caaacaaact gtgcaatgag ctttggctcg
    51961 gaatatgacc taaaccacac tcagcctgca tactgctatt ctgatcaaac agcgcccagt
    52021 cttcgttatt ttcccgcact gctcccagtt cgttgtcggt ttcatccgcc agccgtgggc
    52081 gtttaaagat attattcgct tcaataatgc ctaacatatg tccccacatc cgcgctttgt
    52141 cggaatcagg gctggtgatc accgtaaaaa taacgtcttt ctcatccgag gcggtataat
    52201 cactgcgcat tttcgcggtg atgtgtgttt tcacccccac gccgcctggc tgggtcagcg
    52261 tcagagaggc ctctccgtta gcatcagtga tcccttcata ggtatgcgcg tcaactttgc
    52321 gaacgttact gccaccatca atttgtaccg ggtgcgtctc ccacgcagcg tcagtttgat
    52381 tcttacggtt aacaccgtca tccagatgca gcgaaaagta gtagtaagcc agcggctgat
    52441 cgttgttctt gctatcggta atcgtcaggc gtaaagaagc ctcttccccc acctttactt
    52501 tcatcgcttt tatcgtcgaa tcataactgc ccggcgtcag taccagcttc ggttcaacat
    52561 cgggcgcttc tttatcgaca cagctcacaa taaacgtcgt atcgtccggt ttttgcgctt
    52621 cgctgcggtt cgaaacatcc gccgcatagt gctgcccgct catccccgcc agtgatgacc
    52681 agtagtagtt accctgcatc ggccaaccag ccacatcctg cactgacgtt ggcaccaccg
    52741 tcgccagcgc accgaactga cgcattccgg gcaaaatgtc gcaatgttta tccgcaccac
    52801 tccaggtaaa ggtggaccag gtttcattgt gatcgttaac cgtaccgtct tcattatcga
    52861 cttccgccgc cagttttggt cgactaaaag tgtaatcacg ggccttcagc gtatcgggca
    52921 tatgacccca cattgtggcc tgcgggacat ccgggctggt gaggacggta aagatcactg
    52981 ccgtttcact ggtctgagca atacccgcga ggctcgcgac caacggcgtt tttacaccag
    53041 ggccgttggc ctgggtgacc gtaaccgtgg caacaccatt cgcatcggta gtaccacgat
    53101 attccgtcgc ggtggtggtt aattcggtcg tgcccagttt taccggcgca gtattgttca
    53161 ctacaccctg gcggttttca gcatctttgc gtttgataac aaaaggaata ttccccacaa
    53221 cattaccgac gcaatctttg gtggtgaccg ttaacgtgat actgccgcca accgtcgttt
    53281 tcgccgcacc ggaggattcg tcatagcccg cggcgctgac ggaaataagg tcaccctgcg
    53341 cgggcgcaat cttcagcgtc actttctgat cggcaagggt cagaacactt tcatagggat
    53401 agcccgtcga ggtaagtaga tataacagcg gcatggtctg ctgggggtct ggctgcgccc
    53461 aggcgatcag cgaacgcccg gcaaggccct gtgcgcaaaa tgagcgatct gcgactggct
    53521 gtgggtccag taaggtgtcc ggcgtcgcgg catccgccca aactaaagat actgtcggtg
    53581 gcgtttggtt ttcccaacgt ggtgtggcaa tgatatcgcc ttctctgtct tgcgggttgg
    53641 tgacgagcaa tttagagacg ctaacatccg cgctgaattc actcggttta gcggtaaacg
    53701 ctacctcatg cgtctttgca ggatccagaa agacactgcc ctggtagacg ggtatgctgg
    53761 cactgtcggc ggtgggcacg gtaccgttaa attcgccgac cgtatctgaa gagtcttgcc
    53821 atgcagattc tgcccatgac cacccaggca gcattgcgct tatcagcaat gcgcataaca
    53881 caaggatttt attcagggtc agggacatga cagaaactcc accggttgtt aaacggacgc
    53941 ttgtcgcatc aacatatttt gcttaatccg ggattaagca aaatatgcgg aaacagcatt
    54001 cccttgcggg aatgctgctg agtacgcgtt atttgtgttt gtaaataatg tgaagtttgt
    54061 aaccctgtac cccgtcgccg tttgtcggaa tggtaagagc ctctttacca tcgcgcgcag
    54121 gaccgttaag gttggttctg gcttcgtcgt tggtcgcagg gatggccaga ttgttattat
    54181 cgaagcttga gttaacgata ccacctgaag tgcccagctg ctggctgctg ccagaaagta
    54241 cccaacggaa gtcataattg gcggtaacgt cagcatcggt acccgcatca aacttgccgt
    54301 ttttgttctc atccgagtac agtttggcca cgtaggtttt gccaagttcg attggcgtgc
    54361 tgccgttgat aaggttgata ctggtaccat caacaaagat ggcgactttc aggttctggt
    54421 taaccaccgg ccctggcgca acgttatcgc tgtcgcttcc gccgccgctg gcagtggaaa
    54481 tgtcataaag atgcagcgca gtcccgacgt ttggcgtacc ggtctgcgtg gtcggcgtaa
    54541 tttcaatacc gatataacga ccacggtcgt catcggtaag agtgtaactg gtcaccttcg
    54601 ttgccagctc tttcggatcg cccccctgag cgtccttgta gctcatccag cggatagtat
    54661 cgatagtctt tgcgttatcg cccaatcccc caagatccac gtcgccttcg gtatcaccaa
    54721 tttcccaact tacggttaat ttatcacccg agtaaagacg cttgtccgtt tcactgcctg
    54781 tcgggcgagc gccttttaga tcagaagtca acgttacatg gtcggcatct gtcgtagcgc
    54841 ttcccccttc cgaatagagc cacggaatgg ttccctggaa aatattggtt tcgccgctgt
    54901 cgacccaggc gctttcttcc gccagcgcca caggtgccac acaataacct gccagtgcca
    54961 gcgccaatgc gatccgggtc agaccgcgtc ttttatcctg cttcattgat gctctcctta
    55021 atgaaccttc agcgactggc cgactggcct tacgccaatc ttattttttg gtatacagca
    55081 cgcgtaaacc gtatccctgc aagccatctc ccgcttgtgc gccataggtt tgcgctgctt
    55141 cgcggttagt ggccggaatc acaatgtcct cattggaagt gttcgcctgt gcacccactt
    55201 caccattgat gctcttgccg gtaaattccc acttgtagtc gtactgcgcc atctcttcgt
    55261 cagtcagttt ttcgacctgc tggaagacac cgtctttatt gctgtcacgc cacaaaacaa
    55321 atcgataagt gttgttgagc ggcagcggtt ctttatcttt acctatcagg ttgctgggtt
    55381 tagccgcccc cacacgatag ataaatgcat ttagcccacc aatttctgcc ccgaggaaag
    55441 tgacatccac atagttactg tcgtcgtagg gcgcagcttt tgctttccag cgataggtgc
    55501 cgggtaaggt actggagagg gtgacagtac ccttttcttt atcttgttta gcgataaaaa
    55561 cgcaggggtt gtcggtcgag caaggcttgt tggttaacgt cactcgtttg tcctgcgcgt
    55621 cgtaaagccg cggcgtgagg ttatcttccg gcagcgcatc gccgttgcgg tcggtgttat
    55681 cgccaaagct gtcggttaca tcgagatcca tcaccacacc atcaacgcca tctgcctggg
    55741 cgctggcttc gtggctgtgg ttgttctctt ccggcacgtt cgcgatgtta ttaacaatca
    55801 actcaatttt acggtcctgc tgaacggtga tttccaccac accagagttc tgtcggttgc
    55861 ccttttcatc ttccagcgcc agacgtactc gccaggtatt gagtttttct tgttcggctt
    55921 tgtcagagct gtactgccag gcgggcagca ccaggttcca gtggttattg ttaccctctg
    55981 tcagggtatt ctctttgata accggcataa ccagcgtgcg ggcagcattg tttttcgcct
    56041 gccatgacgc agcgacaatt ttgtagtggt ggttgataag cgcgttaatg gtccacttac
    56101 atttttccag cccgaccgcc gcttcaggcg tgtctttaat gacacactcg gggtgttcgt
    56161 tggtggcatc cgcttttaac cacagggtga catcaagcgg atctttttct ttgtattcca
    56221 gaacaataaa gttattacgc tcaacaaaat catgacgact gcccatcaac gagtgagccg
    56281 ctttcacttt atcgctatcc aattgatctt ttagcggaac gccgaactga taattaagag
    56341 tggcattaat gctgacatcg ctgttatctc cggtccccgc tttatagtcc gccccaacgg
    56401 ttagtaatgg caccggttga taattcagtc caagtgttac cgcgtaggga tctttctcca
    56461 aattatccgt accaaaaagg gcaacttcat cgccgtaata ttgctcgaag acaactttgc
    56521 cccccagttg cggataagaa ggtaaccagg cttcggcgcg aatatcccag ccgcgcgcag
    56581 gacgctcctc ataaaaatcg aaatcttcgg agtctttcca gtcagaaagc ggatgataat
    56641 agttgccaga gaattttaaa taatcagtcc acgcttcgcc gcctaaacct aaacggcgat
    56701 gtcctcgggt aaaatcataa tcataaaata tatttccgcc caataaccat tcatcaaaat
    56761 tatatcttac cccaagacca atattcccga ttgtacgatc ttcttttcgc tgcgcagaaa
    56821 attgactgaa ataaacagtg gtttgattat cgtaccaggg aacaaaataa tcgatggaac
    56881 tgccatccag atcgccgccc tggccaatgg agaggttcga acggacctta ccgtagggag
    56941 agagagtgtc ttcaatataa gattgtgcgg ttgaggtgat ttgactttgc agatagcttc
    57001 tggcctgaga ttccagcgcc tcaggggtca agttttcaaa cccttgcgtg gcggagttga
    57061 tgatgtaatc ggctccgcgc tctttcagcg atttaccctt gttggtttgt tcatcctgct
    57121 gtgctgcctg gctacctaaa tcaggtaacg attcgttttg cttaacattt atctctggcg
    57181 ccgcgaaagc agcaccagag ataaatgtta gaataataca gcgtttccat cgcagcatgc
    57241 actttccctg tgacaatgat acagtcaaaa ataagcttta atattgtatc cctgattatt
    57301 cagaaaggca tatcataaag aataaatata ttcttaatta tttaaattag tgatctcact
    57361 caatt
//
LOCUS       DAZGTJ010000022        53953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000022 DAZGTJ010000000
VERSION     DAZGTJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.022"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(85..1497)
                     /gene="pykF"
                     /locus_tag="ACHK8E_001689"
     CDS             complement(85..1497)
                     /gene="pykF"
                     /locus_tag="ACHK8E_001689"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP0343973.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1505..1699)
                     /locus_tag="ACHK8E_001690"
     CDS             complement(1505..1699)
                     /locus_tag="ACHK8E_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0343974.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2054..2263
                     /gene="fumD"
                     /locus_tag="ACHK8E_001691"
     CDS             2054..2263
                     /gene="fumD"
                     /locus_tag="ACHK8E_001691"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP0343975.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            2719..3345
                     /gene="ydhY"
                     /locus_tag="ACHK8E_001692"
     CDS             2719..3345
                     /gene="ydhY"
                     /locus_tag="ACHK8E_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP0343976.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            3366..5468
                     /gene="ydhV"
                     /locus_tag="ACHK8E_001693"
     CDS             3366..5468
                     /gene="ydhV"
                     /locus_tag="ACHK8E_001693"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP0343977.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            5481..6119
                     /gene="ydhW"
                     /locus_tag="ACHK8E_001694"
     CDS             5481..6119
                     /gene="ydhW"
                     /locus_tag="ACHK8E_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP0343978.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            6183..6851
                     /gene="ydhX"
                     /locus_tag="ACHK8E_001695"
     CDS             6183..6851
                     /gene="ydhX"
                     /locus_tag="ACHK8E_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0343979.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            6848..7633
                     /gene="phsC"
                     /locus_tag="ACHK8E_001696"
     CDS             6848..7633
                     /gene="phsC"
                     /locus_tag="ACHK8E_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP0343980.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            7637..8449
                     /gene="ydhT"
                     /locus_tag="ACHK8E_001697"
     CDS             7637..8449
                     /gene="ydhT"
                     /locus_tag="ACHK8E_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP0343981.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(8455..10065)
                     /gene="ydhS"
                     /locus_tag="ACHK8E_001698"
     CDS             complement(8455..10065)
                     /gene="ydhS"
                     /locus_tag="ACHK8E_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP0343982.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(10191..10496)
                     /gene="ydhR"
                     /locus_tag="ACHK8E_001699"
     CDS             complement(10191..10496)
                     /gene="ydhR"
                     /locus_tag="ACHK8E_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP0343983.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(10604..10680)
                     /locus_tag="ACHK8E_001700"
     tRNA            complement(10604..10680)
                     /locus_tag="ACHK8E_001700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10644..10646),aa:Val,seq:gac)
     gene            complement(10685..10761)
                     /locus_tag="ACHK8E_001701"
     tRNA            complement(10685..10761)
                     /locus_tag="ACHK8E_001701"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10725..10727),aa:Val,seq:gac)
     gene            11070..12326
                     /gene="ydhQ"
                     /locus_tag="ACHK8E_001702"
     CDS             11070..12326
                     /gene="ydhQ"
                     /locus_tag="ACHK8E_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0343984.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(12367..13740)
                     /gene="mdtK"
                     /locus_tag="ACHK8E_001703"
     CDS             complement(12367..13740)
                     /gene="mdtK"
                     /locus_tag="ACHK8E_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP0343985.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            13955..14596
                     /gene="ribE"
                     /locus_tag="ACHK8E_001704"
     CDS             13955..14596
                     /gene="ribE"
                     /locus_tag="ACHK8E_001704"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP0343986.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(14636..15784)
                     /gene="cfa"
                     /locus_tag="ACHK8E_001705"
     CDS             complement(14636..15784)
                     /gene="cfa"
                     /locus_tag="ACHK8E_001705"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP0343987.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     KKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(16075..17286)
                     /gene="punC"
                     /locus_tag="ACHK8E_001706"
     CDS             complement(16075..17286)
                     /gene="punC"
                     /locus_tag="ACHK8E_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP0343988.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            17399..18331
                     /gene="punR"
                     /locus_tag="ACHK8E_001707"
     CDS             17399..18331
                     /gene="punR"
                     /locus_tag="ACHK8E_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP0343989.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(18328..19353)
                     /gene="purR"
                     /locus_tag="ACHK8E_001708"
     CDS             complement(18328..19353)
                     /gene="purR"
                     /locus_tag="ACHK8E_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP0343990.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            19652..19741
                     /gene="cydH"
                     /locus_tag="ACHK8E_001709"
                     /gene_synonym="ynhF"
     CDS             19652..19741
                     /gene="cydH"
                     /locus_tag="ACHK8E_001709"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP0343991.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            19907..21076
                     /gene="ydhP"
                     /locus_tag="ACHK8E_001710"
     CDS             19907..21076
                     /gene="ydhP"
                     /locus_tag="ACHK8E_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0343992.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(21222..21803)
                     /gene="sodB"
                     /locus_tag="ACHK8E_001711"
     CDS             complement(21222..21803)
                     /gene="sodB"
                     /locus_tag="ACHK8E_001711"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP0343993.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21931..22758)
                     /gene="mepH"
                     /locus_tag="ACHK8E_001712"
     CDS             complement(21931..22758)
                     /gene="mepH"
                     /locus_tag="ACHK8E_001712"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP0343994.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            23093..23440
                     /gene="grxD"
                     /locus_tag="ACHK8E_001713"
     CDS             23093..23440
                     /gene="grxD"
                     /locus_tag="ACHK8E_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP0343995.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(23491..28107)
                     /gene="lhr"
                     /locus_tag="ACHK8E_001714"
     CDS             complement(23491..28107)
                     /gene="lhr"
                     /locus_tag="ACHK8E_001714"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP0343996.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(28200..28847)
                     /gene="rnt"
                     /locus_tag="ACHK8E_001715"
     CDS             complement(28200..28847)
                     /gene="rnt"
                     /locus_tag="ACHK8E_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP0343997.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(28950..29357)
                     /gene="gloA"
                     /locus_tag="ACHK8E_001716"
     CDS             complement(28950..29357)
                     /gene="gloA"
                     /locus_tag="ACHK8E_001716"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP0343998.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(29438..30535)
                     /gene="nemA"
                     /locus_tag="ACHK8E_001717"
     CDS             complement(29438..30535)
                     /gene="nemA"
                     /locus_tag="ACHK8E_001717"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP0343999.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(30572..31171)
                     /gene="nemR"
                     /locus_tag="ACHK8E_001718"
     CDS             complement(30572..31171)
                     /gene="nemR"
                     /locus_tag="ACHK8E_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP0344000.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            31274..31513
                     /gene="ydhL"
                     /locus_tag="ACHK8E_001719"
     CDS             31274..31513
                     /gene="ydhL"
                     /locus_tag="ACHK8E_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP0344001.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            31562..32458
                     /gene="ydhF"
                     /locus_tag="ACHK8E_001720"
     CDS             31562..32458
                     /gene="ydhF"
                     /locus_tag="ACHK8E_001720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP0344002.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            32539..33060
                     /gene="sodC"
                     /locus_tag="ACHK8E_001721"
     CDS             32539..33060
                     /gene="sodC"
                     /locus_tag="ACHK8E_001721"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0344003.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(33061..35073)
                     /gene="ydhK"
                     /locus_tag="ACHK8E_001722"
     CDS             complement(33061..35073)
                     /gene="ydhK"
                     /locus_tag="ACHK8E_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0344004.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(35073..35930)
                     /gene="ydhJ"
                     /locus_tag="ACHK8E_001723"
     CDS             complement(35073..35930)
                     /gene="ydhJ"
                     /locus_tag="ACHK8E_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0344005.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(35933..36169)
                     /gene="ydhI"
                     /locus_tag="ACHK8E_001724"
     CDS             complement(35933..36169)
                     /gene="ydhI"
                     /locus_tag="ACHK8E_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP0344006.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            36370..36804
                     /gene="slyA"
                     /locus_tag="ACHK8E_001725"
     CDS             36370..36804
                     /gene="slyA"
                     /locus_tag="ACHK8E_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP0344007.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(36851..37318)
                     /gene="slyB"
                     /locus_tag="ACHK8E_001726"
     CDS             complement(36851..37318)
                     /gene="slyB"
                     /locus_tag="ACHK8E_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP0344008.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            37592..38701
                     /gene="anmK"
                     /locus_tag="ACHK8E_001727"
     CDS             37592..38701
                     /gene="anmK"
                     /locus_tag="ACHK8E_001727"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP0344009.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            38799..39128
                     /gene="mliC"
                     /locus_tag="ACHK8E_001728"
     CDS             38799..39128
                     /gene="mliC"
                     /locus_tag="ACHK8E_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP0344010.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            39187..39843
                     /gene="pdxH"
                     /locus_tag="ACHK8E_001729"
     CDS             39187..39843
                     /gene="pdxH"
                     /locus_tag="ACHK8E_001729"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP0344011.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            39972..41246
                     /gene="tyrS"
                     /locus_tag="ACHK8E_001730"
     CDS             39972..41246
                     /gene="tyrS"
                     /locus_tag="ACHK8E_001730"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP0344012.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            41305..42168
                     /gene="pdxY"
                     /locus_tag="ACHK8E_001731"
     CDS             41305..42168
                     /gene="pdxY"
                     /locus_tag="ACHK8E_001731"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP0344013.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(42212..42817)
                     /gene="gstA"
                     /locus_tag="ACHK8E_001732"
     CDS             complement(42212..42817)
                     /gene="gstA"
                     /locus_tag="ACHK8E_001732"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP0344014.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(42923..44425)
                     /gene="dtpA"
                     /locus_tag="ACHK8E_001733"
     CDS             complement(42923..44425)
                     /gene="dtpA"
                     /locus_tag="ACHK8E_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP0344015.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(45036..45671)
                     /gene="nth"
                     /locus_tag="ACHK8E_001734"
     CDS             complement(45036..45671)
                     /gene="nth"
                     /locus_tag="ACHK8E_001734"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP0344016.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(45671..46366)
                     /gene="rsxE"
                     /locus_tag="ACHK8E_001735"
     CDS             complement(45671..46366)
                     /gene="rsxE"
                     /locus_tag="ACHK8E_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP0344017.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(46370..46990)
                     /gene="rsxG"
                     /locus_tag="ACHK8E_001736"
     CDS             complement(46370..46990)
                     /gene="rsxG"
                     /locus_tag="ACHK8E_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP0344018.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(46994..48052)
                     /gene="rsxD"
                     /locus_tag="ACHK8E_001737"
     CDS             complement(46994..48052)
                     /gene="rsxD"
                     /locus_tag="ACHK8E_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP0344019.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(48053..50275)
                     /gene="rsxC"
                     /locus_tag="ACHK8E_001738"
     CDS             complement(48053..50275)
                     /gene="rsxC"
                     /locus_tag="ACHK8E_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP0344020.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKACKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(50268..50846)
                     /gene="rsxB"
                     /locus_tag="ACHK8E_001739"
     CDS             complement(50268..50846)
                     /gene="rsxB"
                     /locus_tag="ACHK8E_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP0344021.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(50846..51427)
                     /gene="rsxA"
                     /locus_tag="ACHK8E_001740"
     CDS             complement(50846..51427)
                     /gene="rsxA"
                     /locus_tag="ACHK8E_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP0344022.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(51504..51944)
                     /gene="ydgK"
                     /locus_tag="ACHK8E_001741"
     CDS             complement(51504..51944)
                     /gene="ydgK"
                     /locus_tag="ACHK8E_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP0344023.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(52030..52245)
                     /gene="ydgT"
                     /locus_tag="ACHK8E_001742"
     CDS             complement(52030..52245)
                     /gene="ydgT"
                     /locus_tag="ACHK8E_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP0344024.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(52518..52643)
                     /gene="blr"
                     /locus_tag="ACHK8E_001743"
     CDS             complement(52518..52643)
                     /gene="blr"
                     /locus_tag="ACHK8E_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP0344025.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            52886..53926
                     /gene="ydgJ"
                     /locus_tag="ACHK8E_001744"
     CDS             52886..53926
                     /gene="ydgJ"
                     /locus_tag="ACHK8E_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0344026.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
BASE COUNT        13603 a        13269 c        13997 g        13084 t
ORIGIN      
        1 tttttattgc ttctggttat cgattaaata aaaaaagcgc ccatcagggc gcttcgatat
       61 acaaattaat tcacaaaagc aatattacag gacgtgaaca gatgcggtgt tagtagtgcc
      121 gctcggtacc agtgcaccag aaaccataac tacgacgtca cctttgtgtg ccagaccgct
      181 ctgcagagcc agttctttac ccagacggta gaaatcatca gtagaagtga tctctttaac
      241 cagctgcggc acaacgcctt tgctcagtac caactgatga gccgtttttt cgttggtggt
      301 cagtgccagg atggtggcat ccgggaagta tttacgtact gcgcgagcag atttaccgcc
      361 ctgggtagca accacgatca gcggggcatc cagtttttca gcagtttcaa cggcaccacg
      421 gcataccgct tcggtaatgc gcagtttacg gttgtcattg ttgaactcga gacggctgtt
      481 catcacgcgg tcggtacgtt cgcagatggt cgccatgata gaaaccgctt ccagcgggta
      541 tttacctttt gcggattcac cagacagcat cactgcgtca gtaccgtcga ggatggcgtt
      601 tgcaacgtca ccggcttctg cgcgagtcgg gcgtgggttt ttaatcatgg aatccagcat
      661 ctgggtcgca gtgataacga ctttacgtgc acggatacat ttttcgatca tcatcttctg
      721 ggcgaagata acttcttcta ccgggatttc tacacccagg tcgccacgcg caaccatgat
      781 gccgtcagag gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt
      841 ggagatgatg tggatgtttt cgccgccgtg cgctttcagg tgctcacgga tttcgataac
      901 gtcagaacgc ttacgaataa aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa
      961 gatcaggtcc tgtttgtctt tttcagccag tgctggcagg gcaatggaaa cgccaggcag
     1021 gttcacacct ttgttttcac ccaggtcgcc gttgttcagc actttacaga taactttgtt
     1081 accttcaatg gcggtaactt ccataccgat gagaccatcg tcaaccagta cggtgttgcc
     1141 aacagacagg tcagtagtga aaccttcata cgttaccgca accatttcgc tgttgccgat
     1201 aacagattta tcagtggtga aagtaaaggt ctgacccgct ttcagagaaa cgtcgttacc
     1261 gccttccagt ttcatggtgc ggatttccgg acctttggta tcaagcagga tagcggcggt
     1321 tttaccagtt ttgctcatca cgttgcgcag attctgaatg cgctgaccgt gttctgcata
     1381 gtcaccatga gagaagttca gacgcataac gttcatgcca gcgtccagca ttttagctaa
     1441 catctcttca gattcggttt tcggtccgat ggtgcaaaca attttggtct ttttcatgac
     1501 agtcttagtc tttaagttga gaaggatggg agaaacttgc tttctgggcg cactgccgga
     1561 aagcgaatcc ggtattacga aagtggtggt gcctcgctct aaggataggt gacatcgaaa
     1621 aagcgtgcag aggaatgtgt gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc
     1681 gttgagttct acagtccaaa gtgctgaaac cattcaagag tcaattggcg cgcattatac
     1741 gctgaaatta atccaaaagg aaaacaaaaa cagcgtttca aaaaatatac aacttgttag
     1801 acaataaatt gttatcaaag atttaacgtt ccagggaaaa ggttacgcaa aaacttcgct
     1861 gtttgcgcaa cgtctggtgc cggggttgtc actgtgaaga taagtgaaag ttcatccggt
     1921 gtataaatac atcatcgctc aattgctaca gttaaaataa tttatcgcgt tatttgtctg
     1981 attttgtttg atttttgttt aattggttag attgatatat gctgatagtt ggcatgataa
     2041 ataggtataa agaatgggaa atcggacaaa agaggatgaa ctgtaccgag aaatgtgcag
     2101 agttgtcggt aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa agcatattgt
     2161 tatcgcaggc gtattacgta cagcattagc caataaacgc attcagcgca gtgagctgga
     2221 aaagcaggcg atggaaaccg tcattaacgc actggtaaaa tgaccattca ggatgaggcg
     2281 gtaagcttca tcctgatatt cttgcttcag ttgcggtcca gttaatcatt ctaaataaca
     2341 actgctgaaa ttatacgaat ttcggtgtgt attttaattt atagcgataa agttattttt
     2401 attttctgcg atgaacttca caaataattg tcggttaagt taaatttatt tcaaatgata
     2461 ataaatatca gttcacttat aatttcttgc tttgtgattt taattgataa cgatcaatgt
     2521 taagagccca gaataatcac attatttttt ctcctaacca taaaggatta gtttatcagc
     2581 gttaaatctt ttctcataca ctattgggtt agggaaaata tctggtgttg aggacagagt
     2641 cagcagcgta ttagttgagt gcctaatatc aggctgctga attgtcagca ctgtgtttgc
     2701 gaagaggatg cgaaataaat gaacccggtt gatcgtccac tattaaatat tggcttaacg
     2761 cgattagaat tcttgcgtat atcaggaaaa ggccttgctg gtttaacaat tgcccctgcg
     2821 ttactgtcac ttttgggttg caaacaggaa gatattgata gcggcacagt aggcttgata
     2881 aatacaccca aaggggtgct ggtgacccat cgtgcgcgat gcacgggttg ccaccgctgt
     2941 gaaatctctt gtaccaactt caacgatggc tcagtaggga cattcttctc ccgtatcaaa
     3001 atccatcgca attatttctt tggcgataac ggggttggct ctggtggcgg cctgtatggc
     3061 gatctcaact ataccgcgga cacctgccgt caatgcaaag agccgcaatg catgaacgtc
     3121 tgcccgattg gtgcgattac ctggcagcag aaagaaggct gtattaccgt cgatcataaa
     3181 cgttgtattg gctgtagcgc ctgtactacg gcatgcccgt ggatgatggc caccgtaaat
     3241 accgaaagta aaaaatcctc gaaatgtgtg ttatgcggtg aatgcgcaaa cgcctgccca
     3301 acaggggcgt taaaaattat cgagtggaaa gatattactg tgtgaatctt gcaaaggaaa
     3361 aaatcatggc taacggttgg acaggtaata tattaagagt caatctcacg acaggaaata
     3421 ttaccctcga agattccagt aagtttaaaa gttttgtcgg tggcatgggc ttcggctaca
     3481 aaattatgta tgacgaagta ccgccaggca cgaaaccttt cgatgaagcg aataaattag
     3541 tttttgctac cggcccatta actggatctg gtgccccctg tagttctcgc gtaaatatca
     3601 cctcactttc tacttttacc aaaggaaatt tagtcgtcga tgcccatatg gggggctttt
     3661 ttgcagcgca aatgaaattc gctggatacg acgtcattat tatcgaaggg aaagcgaaat
     3721 caccggtatg gctgaagatt aaagatgaca aagttagcct tgaaaaagcc gatttcttat
     3781 ggggaaaagg gacgcgcgca acgacggaag aaatttgtcg cttgaccagt ccggaaacct
     3841 gtgtggcggc tattggtcag gctggggaaa accttgttcc tctctctggc atgttgaata
     3901 gccgtaacca cagcggtggt gcgggaactg gcgcaataat gggttcgaaa aacctgaaag
     3961 cgattgcggt tgaagggacg aaaggggtca acattgccga tcgtcaggag atgaagcgtc
     4021 tcaacgatta catgatgact gaacttatcg gtgcgaataa caaccatgtc gtgccaagta
     4081 cgccgcaatc gtgggcagag tattcagatc ccaagtcacg ttggactgca cgtaaagggt
     4141 tgttttgggg cgcggctgaa ggtggtccga ttgaaacggg tgagattccg ccaggcaatc
     4201 agaacacggt cggctttcgt acctataaat ccgtttttga cttaggtccg gcggcagaga
     4261 aatacacagt aaaaatgagt ggctgccact cttgcccgat ccgttgtatg acccaaatga
     4321 atattcctcg ggtgaaagag tttggcgtgc ccagcacagg tggtaacact tgtgtagcaa
     4381 actttgtcca taccaccatc ttcccgaacg gtccgaaaga ttttgaagat aaagatgatg
     4441 gtcgtgtgat tggtaacctg gttggtctga atctgttcga tgactacggc ctatggtgta
     4501 actatggtca gcttcatcgc gactttacct attgttacag caaaggggtg ttcaagcgtg
     4561 ttctgcctgc tgaagagtat gcggaaattc gctgggatca actggaagcg ggtgacgtta
     4621 acttcattaa agatttttac taccgtctgg cgcatcgcgt gggtgagctg agtcacctgg
     4681 ctgatggttc atatgccatc gcagaacgct ggaatttggg tgaagagtac tggggctacg
     4741 cgaaaaataa actctggtcg ccgtttggct atccagttca ccacgccaat gaagcgtcag
     4801 cgcaggtcgg ctccattgtt aactgtatgt tcaaccgtga ctgcatgacg catacccata
     4861 tcaactttat tggttccggc ttgccattga aactgcaacg tgaagtggcg aaagaacttt
     4921 ttggttctga agatgcttac gatgaaacca aaaactacac gccaatcaat gacgcaaaaa
     4981 ttaaatatgc taagtggtcg ctgttgcggg tctgtttgca taacgccgtc accctgtgca
     5041 actgggtctg gccaatgacc gtttcgccgc tgaaaagccg taattatcgg ggcgatctgg
     5101 cgcttgaagc caaattcttc aaagcgatca ctggcgaaga tatgactcag gaaaaattag
     5161 atttagctgc agagcgtatc tttacgttgc atcgcgccta cacggtaaaa ctgatgcaaa
     5221 ctaaagatat gcgtaatgaa cacgatctta tctgttcctg ggtattcgac aaggatccgc
     5281 agatcccggt ctttactgaa ggtacagaca aaatggaccg tgacgatatg catgcttcgc
     5341 tcacgatgtt ctacaaagaa atgggctggg acccacagct tggttgtccg acccgcaaaa
     5401 cattgcagcg tctggggctg gaagatattg ccgccgatct ggcagcacac aatctactgc
     5461 ctgcgtaagg aatgggtgag atgaaccatc aggatgaatt gccgttagcg aaagtcagcg
     5521 aagttgatga ggcgaagcgg caatggttgc aggggatgcg tcaccccgtt gatacggtaa
     5581 ccgaaccaga acctgctgaa atcctggcag agttcattcg tcagcattct gcggctgggc
     5641 agctggtggc gcgcgccgtg tttctctcgc cgccctattc ggttgcagaa gaggaactgt
     5701 cagttttgct ggaaaacatt aagcagaacg gagaccacgc ggatatcgcg tgtatgacgg
     5761 gaagccagga tgattactat tactcaacgc aggccatgag tgaaaactac gcggcgatgt
     5821 ctttgcaagt tgtggagcag gacatttgtc gcgccattgc acacgccgtt cgttttgagt
     5881 gccagacgta tcctcgtcct tacaaagtcg ccatgctgat gcaagcgcca tattacttcc
     5941 aggaagcgca gatcgaagcc gcgatcgccg cgatggatat tgcacctgaa tatgctgata
     6001 tacggcaggt ggagtcatcg acggcggtgt tgtacttgtt tagcgagcga tttatgacct
     6061 atggcaaggc gtatggctta tgcgaatggt tcgaggtaga gcagtttcag aacccctgaa
     6121 atttcaggga gatgtcatgg atagggtgga cagtagcagc cactgcatta ggggataatc
     6181 agatgtcctt cactcgacgc aaatttgttc tggggatggg aacagtaatc ttttttactg
     6241 gttctgcgtc ttctctgtta gcgaacacga tgcaagaaaa gaaagttcgg tatgccatga
     6301 ttcatgacga gtcacggtgc aatggctgta atatttgcgc ccgcgcatgc cgtaaaacta
     6361 accacgtccc cgctcaggga agccgtttat cgatagcaca cattccagtc accgataacg
     6421 acaacgaaac gcagtatcac ttttttcgcc agtcatgcca gcactgtgaa gatgctcctt
     6481 gcatcgacgt ttgcccgaca ggggcgtcgt ggcgtgatga gcaggggatc gtgcgggtag
     6541 aaaaatcgca gtgcatcggc tgcagttatt gcattggcgc ttgtccttac caggttcgct
     6601 accttaaccc tgtcactaaa gtggcagata aatgtgattt ctgcgctgag tcccgactgg
     6661 caaaaggctt tccgcccatt tgcgtcagcg cctgtccgga acacgcattg atctttggtc
     6721 gcgaagatag cccagaaatt caggcgtggt tgcaggaaaa taaatactat caatatcagc
     6781 tacctggtgc cgggaaaccg cacctgtatc gccggttcgg tcaacatttg attaaaaagg
     6841 aaaatgtatg aacccgtcgc aacatgctga acagtttcag agccagttgg cgaactatgt
     6901 gccactgttc actccccaat tttggccggt gtggttgatc attgccggac tattgctggt
     6961 agggatgtgg ttggtgctgg ggctgcatgc cttgcttcgt gctcgtggcg tgaagaaatc
     7021 agccaacgac catggtgaga aggtttatct ttacagcaaa gcggtcagat tatggcactg
     7081 gtcgaatgcg ttactctttg tattgttgct ggcaagtggg ctgataaatc actttgcgct
     7141 ggtgggcgca actacggtta aaagtctggt tgcggtgcat gaagtttgcg gatttttgtt
     7201 actggcatgc tggctcggct ttgttctgat caatgccgtt gggggtaatg gtcaccacta
     7261 tcgcattcgt cgtcaggggt ggctggagcg agcggcaaaa caaacgcgat tctatttgtt
     7321 tggcattatg cagggggaag aacatccttt cccggcaaca acccagtcta aatttaatcc
     7381 cttacagcag gtcgcctatg ttggtgtcat gtatggattg ctgccattgt tactattgac
     7441 ggggctgctg tgtctctatc cgcaagccgt gggagatatg tttcctggcg taagatactg
     7501 gttattgcag gcgcattttg ctctggcatt tataagcctc ttttttatct tcggtcatct
     7561 ttatctttgc accacggggc gtacgccaca cgaaaccttt aaaagcatgg tcgatggcta
     7621 tcaccggcac taacgaatga tcattacacg agcagacctg cgagaatggc gtatcggagc
     7681 ggtgatgtat cgctggtttc tgcgccattt cccacgcggc ggtagttatg ccgatattca
     7741 tcatgcactg attgaggaag gatatacgga ctgggcggag agtctggttg agtatgcgtg
     7801 gaaaaaatgg ctggcggatg aaaacttcgc gcatcaggaa gtttcttcta tgcaaaaact
     7861 ggccacagat cccggagaga gagcattttg cagtcagttt gcccgcagcg acgatcatgc
     7921 tcgcatcggc tgttgtgagg ataatgccag aatcgcaacc gctgggtatg ccgcgcaaat
     7981 tgccagtatg ggttatagcg tgcggattgg aagtgtcggc tttaacagtc acattggcag
     8041 ctccggtgaa cgcgcacggg ttgctgtaac cggtaattct tcgcggataa gcagcgctgg
     8101 agatagcagt cggattgcga ataccggaat gcgagtacgc gtttgtacat tgggtgaacg
     8161 atgtcatgtc gccagtaatg gtgacctggt gcaaatcgcc agctttggcg caaacgccag
     8221 aattgccaac agcggcgaca atgtacacat cattgccagc ggtgaagatt caacaatagt
     8281 gagcacgggc gtggtcgact caattattct cgggccaggg ggaagcgccg cactggcata
     8341 tcatgacggc gaaagagttc gcttcgccgt cgccattgaa ggtgaaaaca atattcgagc
     8401 cggagtccgc tatcgcctta atgaacagca tcagtttgtt gagtgctgat agctttacat
     8461 ttgattaaac gaaagacgcc gacgagcacg ggatgcaggc tttacgaccg cctgagctat
     8521 cgcctcacca atttctgcac atgccgtaag tccctgtacg aatggctggt cgtgcatcaa
     8581 ccagggcaac gcgccgaacg ctacgcgccc acgaatttct tcgggttgct gtagcgtata
     8641 atcttcgcca acatcaggga tttcatcccc tgttttctgt aattgttcgc gtagtccagg
     8701 gaaaggaata tctttcactt taagcggacg ctgcccacgg gcatcaataa aaacgtcaaa
     8761 cgagtagcta ttgtcttccg ttttcaggac ggtgcgcgac tcattaattt ccatttcgta
     8821 gtcttcaccg agggcgagaa tatgaatgat tcccgcttca cgtaaggcca gtagtcgacg
     8881 aatagactct gaagggattg ccgcataatt atcgatgaaa acccgggcaa ggcctttact
     8941 gaaccgttta tggtcgtgtt cattcagatg aggaacaatt tcctgtacgg cttcatgcag
     9001 gcgcagaatg acataacgcc agggaacagt atgtttttct cgtttattgc gttcgacttc
     9061 ctggagattt ttttctgccc agtcgaatgg gtcgcgttgt ttgcgctcgg caaaccaggc
     9121 ttgagcaaag gaatcgacat tcaggctttc taaggctatg cgttggctcc agtctggatc
     9181 agcaaatttg atttcctcta ctatcaatct aaatacccga tccaaaaggc ccacttcgcc
     9241 tttttgaatc tcagcattta atgcttgatc ggtgacgatg tgtaagggct cgtagggaat
     9301 agggcaatag aaatcggctt cgggtaaaat acccgtacgc gacatcaacg taatatttag
     9361 cttttcactt gcgttatcgc ggtgaaaaac gacgtgttgt ttatcatctt caatgaacga
     9421 accatgctga atagccactg ccattgccgc atccagtccg ctcaaggatg ttcccataat
     9481 acccacgtta cacgcatcga cctttgcttc catcaggcct gaccacgggc tgggaaaata
     9541 cgttcgggtt gcttcttctt catcaggcca gacgtgaccc gtggcgatca ccgctaaatc
     9601 aaacgtctcg ctgggtaaat cctgatttgt agcgagcatg acgccagcat ttgtaatttg
     9661 cagatcggta acctggcatg attcataaac agccactgca aatttttgct gtcgtgcctg
     9721 gtctactaat cgtaaaaatt gatcgcggaa atattcgccc agcagaattc gcggtaaaaa
     9781 ctgacgatcg tgcaaggttt cttttttaac gccataacgc tggagatggc tggcttcttg
     9841 cttttgtagc cattcgagat acgtacaata aatcggcggt atttcaatac tggcaatatt
     9901 tgccagcatc atttttgagt tttcctcatc actgtatggc atcccgacac cggcttcatc
     9961 agcctgctcg aagatagaaa tagaaagtgg agtttgttgc tgtagaagcg agaataaggt
    10021 gtagatcccc gtaggcccgg cacccacaat agcaattttt ttcattgtta cttacctgta
    10081 cgcattcttt cgaatttagt gttatcagat aagtttggtt gagattttta gaatttgaga
    10141 aatttcagac agggatgaaa agggcgctaa ccgcccttga ttaaattctg tcaggcgagt
    10201 tttgcctgat tgatttgact aagtggctca ttgacatcga aaactttggc gacaacttcc
    10261 tcaacgccga gatttttcag tcgggcagta tgtttttcca gataggcaag ggcgcttttt
    10321 tcattagtaa aaaggtagat cccaccggct tcgtggttct tttcactttc tgtccatacc
    10381 ttccacagaa aaccaggttc ctgattaatc gactcagcaa gcggtttaag ctgctcagcc
    10441 attgcgtcgc caaatgggcc attgaaagaa aaatgaagtt gtaacaaagt tgccatagcg
    10501 attctccttc tgataacgca tacgaagaca gtgtaaatct tttaacgtat tggatataga
    10561 gaagaaacag agggaaaaag gagaagacca gattaagaaa atctggtgcg tccgagtgga
    10621 ctcgaaccac cgacccccac catgtcaagg tggtgctcta accaactgag ctacggacgc
    10681 aggatggtgc gttcaattgg actcgaacca acgaccccca ccatgtcaag gtggtgctct
    10741 aaccaactga gctatgaacg caacgttgta ggtgacaacg gggacgaata ttagcggcag
    10801 agtgggaagg tggcaagagg caaaacgtaa ttttctgcgc tatttcgacc gtttgcagag
    10861 cttttaagca aattggctat attttgttga tttgcaaggg cgatttttat tcaggatcgc
    10921 atttacatca gatacaaccc aataatccgt gtatcagagt ttaaacagag ttgtttgcca
    10981 ccatctcaaa atctgtcgtg gataaatcct ccgtagcctt tacacttagg tagcaataaa
    11041 ggcacacaga tgcagcaaag gaataaatca tgggatctga tgcgaaaaac ttgatgagcg
    11101 acgggaacgt gcaaattgtt aagaccggcg aggtcattgg cgcgacgcaa cttactgaag
    11161 gtgagttaat tgttgaagct ggcggaagag ccgaaaatac cgtggtcacg ggggctggct
    11221 ggttgaaagt ggcaaccggt gggatcgcca aatgcacaca gtacggcaac aatggcacgc
    11281 tatcggtcag cgacggtgcc attgccacag atattgttca gtccgaggga ggcgcaatta
    11341 gtctctctac gctcgctacg gttaatggcc gccatcccga aggtgaattc agcgttgata
    11401 aaggttatgc ctgcggtttg ttgctggaaa atggcggtaa cctgcgtgta ctggaaggcc
    11461 atcgcgcgga aaaaattatt ctcgatcaag agggtggcct gttggtcaat gggacaacct
    11521 cagcggtcgt ggtagatgaa ggtggtgaat tgttggtgta tccaggtggg gaagccagca
    11581 attgtgagat taatcagggc ggcgttttta tgctggcggg gaaagccagt gatacattgc
    11641 ttgctggtgg caccatgaat aatctcggtg gtgaagactc tgacactatt gttgagaatg
    11701 gatccactta tcgtctgggg acggatggtc ttcagctcta cagttccggt aagacgcaaa
    11761 acctgtccgt taatgtgggt ggtcgggctg aagtgcatgc cggtacgctg gaaaatgcgg
    11821 taatacaagg tgggacagtc atcctgttgt cacccaccag cgcggacgaa aattttgtcg
    11881 tagaggaaga tcgcgcaccg gttgaactga ccggtagtgt tgcattactg gacggcgctt
    11941 caatgattat tggctatggc gcagatctgc aacaatcaac gattactgta cagcagggcg
    12001 gtgtattgat tctcgacggc agtacggtaa aaggtgacag tgtcactttc agtgttggta
    12061 gcatcaatct aaatggcgga aaactgtggc taatcactgg tgcggcaacg catgtgcaac
    12121 tgaaagtgaa acgcctgcgc ggagagggag cgatttgcct gcaaaccagt gcgaaagaaa
    12181 tttcacctga cttcatcaat gtgaaagggg aagttactgg tgatatacac gttgagataa
    12241 cagatgccag tcggcaaact ctgtgtaacg cactgaaact acagccagac gaagacggga
    12301 ttggcgcaac gctccagcct gcgtaaaaac acaggcgcaa cccgtgaagg ttgcgccttg
    12361 tctttactag cgagctgctc gttgcagaat gatggctgac ggcagacgtt gcaggaaccg
    12421 catacgcagc atcatcataa tggctgccga cgtcaggcca ataataaagc ctatccagaa
    12481 gcctgctggc cccataggtt caacgaccag atcggtcagt gccagaatat agccgcttgg
    12541 caagcccagc acccagtaag ccgtaaaggt aatatagaaa atggaacgcg tatctttata
    12601 tccacgcaaa atcccactgc caatcacctg gattgagtca gaaatctgat ataccgccgc
    12661 cagcaacatc aaatgcgcag ccagcgttac aacctcggga ttgtcgttgt acaacagggc
    12721 gatttgctcc cgcagtgaaa ccgtgaaaat ggccgtcagg gttgccatac agacacccac
    12781 cataagcccg gtccgcgcag cggtttgcgc atccagcgtt gagccctgac ccagacgata
    12841 acctacgcgg atggttaccg ctgccgccag cgacatggga agtacgaaca tcagtgaact
    12901 aaagttcagg gcaatctgat gtcctgcgac atcaacaata ccgagcggag acactaacag
    12961 agccacgacg gcaaacagcg ttacttcaaa gaacagcgcc agcgcaattg gcaaaccgag
    13021 ttgaatcagt cgtttcataa ccgcaggatc gggcttcgca gtgccttttt cgttacgaat
    13081 atcgcgcatc gagcgggcgc gtttaatgta agaaaccatg gcaaggaaca tgacccaata
    13141 caccgcagca gtagccacgc cacaaccaac gccaccgagc tcaggcatac cgaaatgacc
    13201 gtaaataaag atatagttca ccggaatgtt caccagcagg ccgataaagc ccatcaccat
    13261 acccggcttg gtttttgcca gaccttcaca ctggttacgg gcaacctgga agaaaagata
    13321 gcccggcgcg ccccatagca acgcacgcag ataacccacg gctttgtccg ccagagccgg
    13381 atcgatgttt tccatggagc ggataatgta acctgcattc cacagcacca gcataatgag
    13441 aacggaaaca aaacccgcca gccagaaacc ttgtcgcacc tgatgcgcaa tgcgctcacg
    13501 tcgaccggaa ccatttaatt gcgcgataac cggcgttaat gccagcagca gtccgtgacc
    13561 aaagaggatc gccggaagcc agatagaagt accgatagcg accgccgcca tgtcggtggc
    13621 actatagccg cccgccatca cggtatcgac aaaacccatc gcagtttggg cgatttgcgc
    13681 gagaatcacc gggattgcta atgctaataa cagacgcgct tcactgatat acttctgcac
    13741 gtgaacacct tttatttgta gttatatgag agactaaaaa gccgcctgaa tgggcagcaa
    13801 aaagaagaag caggggaaat tcagtctatt gtagcgagga tttattaatt ctccagtgaa
    13861 aaaatagtca ttcttacccc ttcactggta agggcgtttg ccacctgcta ttgtgctgaa
    13921 cagaatgtct taactgattt caggagttgt aagtatgttt acggggattg tacagggcac
    13981 cgcaaaactg gtgtcgattg acgagaaacc aaattttcgc acacatgtgg tggagttacc
    14041 cgaccacatg ctggacggtc tggaaaccgg tgcttccgtg gcgcataacg gttgctgcct
    14101 gaccgtgacg gaaattaacg gcaaccatgt cagttttgac ctgatgaaag aaacgttacg
    14161 cattaccaat cttggcgatt taaaagtggg ggattgggta aacgttgagc gtgcggcgaa
    14221 attcagtgat gaaattggcg gacacttaat gtcaggtcat attatgacca ccgctgaagt
    14281 ggcgaaaata ttaacctcgg aaaataatcg ccagatctgg tttaaagtcc aggatagtca
    14341 gttgatgaaa tatattctgt acaaaggatt tattggcatc gacggtatta gcctgaccgt
    14401 cggtgaagtc acgccaacgc gtttttgcgt ccatttaatt ccggaaacac tggaacgcac
    14461 gactcttggg aagaaaaaac ttggcgcacg cgtcaacatt gaaatcgatc cacaaactca
    14521 ggcagtggta gatacggtag aacgagtgct ggcggcacga gaaaatgcca tgaatcaacc
    14581 aggtacagaa gcctgatctg aaatcgcccc cggagagggg gcgatagaat agcctttagc
    14641 gagccactcg aaggccgttt tcaacaccgc gtgagaacac gacctgccag agctgaatat
    14701 cacgggcgcg gaaagcaccc gcacaggcat tcagataata ggtaaacatc cgtttaaagc
    14761 gttcactgta gttatccgca atttctggcc atgcggcgag gaatcgttca taccacgcca
    14821 tcaacgtagt atcgtaatca gcaccgaagt tatgccagtc ttccatcaca aagtggggtt
    14881 cgctggactg agcaatctgg cgtacagagg gcaggcaacc gttcggaaaa atatatttat
    14941 taatccaggg atcaacattc agatcggttt ttttcgaacc gatagtatgg agcaggaata
    15001 tgccttccgg tttcaaatta cgatccacca ccgcaaaata ggtatcgtaa tttttcggtc
    15061 cgacgtgctc gaacatcccc acagaaacaa tacgatcaaa ctggtcgttc aggtcacgat
    15121 aatcttgcag caaaatggtg acatccaggc cttcacagcg ttcctgagcc attttttgct
    15181 gttcggcaga aatggtgacg ccgaccacgc ttacgtcata attagacgcc atgtagtgcg
    15241 ccagtccacc ccagccacag ccaatatcca gtacgcgcat ccctggtttt aactgcaatt
    15301 ttttacaaat cattttgagc ttcgcctgct gggctgattc cagattatcg gcatctttcc
    15361 agtaagcaca ggaatattgc atgaagggat caagcatgcg gctgaacaag tcattaccca
    15421 aatcgtaatg ctctttgccg actatccagg cacgtttttt actctgcaga ttgaagagac
    15481 gagcgccggc aatacgcagc gtgtctttga aatgatgggg gagttggttc tcgagacctg
    15541 cgcgtaagac tttgctaaaa aacatatcca gtcggtcaca ttcccaccag ccatccatat
    15601 aactttcgcc taaccccaac gatccttcct gcagaacgcg tttaaaaaaa tcggggtttt
    15661 tcacacgaat atccgccggg gcagaaccgt taatggctat accggcacgg ctaagtaatt
    15721 cgttggcgat acggtaccag ttgtcatccg gtacactgac ttcttctata cacgatgaac
    15781 tcatagtttc tccatcactg gtcggtgatc agaaccttaa aacagcgtag acgctttttg
    15841 ggctttgtga gaaatctcgc agaaaaaacc gcgagtctat aatccgacgt agaacagagg
    15901 gaaaggagga acccttgccg aaatgaccat ccatagtgag tcgggaacga tcctgtcccc
    15961 gtaaaaacat aatatttatc gtagctttaa caactcgaaa ttagtatagg ccgctaattt
    16021 caatcataca acaaaatttt gttagcagcc taagtataat catatcgata tagatcagtg
    16081 tgattcgcta tgagcgactt cggcattgcc atgattctga cagtctactt cttcacaacg
    16141 ttgcatcatg taacccagag cgaccagcac tactgttgat aacatcacgc tggtggtggt
    16201 gagcaatggc gtgctgatac tgatcagcca ggaaactacc agacttgcga ggaagcacag
    16261 acccagttgt agagtgttct gcaacgctgc ggcgcgaccg gttgcatgtg ggaagggacg
    16321 cagcgcctgg gcgacaacaa tcgggtagat cgcgccattg gcaatcgcca tcacacagaa
    16381 tgggatcagg atttcgacta gcgacacatg gctaatgaag cccgcagccc aggtcgcaat
    16441 gacgctgaca gcaaacagta ccagcaacca cggtaataac tgcttgcctt gccatttctg
    16501 cagtgcggcg cgacagccat aaccaccaat cagaaacgcg atagtttgcg ggacataact
    16561 taaaccaata actgccgggc tgtagcccat ttcactaagg atgaacggtg agccggtcag
    16621 ccatgcaaaa aaactggctg aacaggccgc atatatcagc acgttgccgc gataggtttt
    16681 agaacgtagc aggtcggtaa aggtcagacc atcctgacta ttgttacggg ccttcgtcgt
    16741 gggtttgagc cagaaaatag gcagaatcag caccacggta atggcaaaca gggtggcgaa
    16801 aatcgcctgc caggaaaaat ggaccagcag ccagcttcct aacagaggag ccagtgccgg
    16861 agatagaccc accagcggca tgatggtcgc aaaaatacgg ttaactttct gtgaaggata
    16921 ataatccgtc actaacgctt gccagataac tgccgcggcg cagacaccca cagcctgtac
    16981 aaaacgcaat accagcagcg tagcggcgtt ttctacccac agcatcccca gactacctaa
    17041 cgcaaaaatt gtcaggccga ttaataatac cggtttacga ccataacggt cggagagcgg
    17101 cccccacaga agctgggctg cggcaaaacc ggcaaggaac agactaaggc tggcactgac
    17161 agcagacgca ggcgtttgca ggtcggcctg tatggcggcg aaagcaggca gatacatatc
    17221 ggttgccaga aaaccgagta cgctcaaacc cgccagccag actaaaaatc ttttcccagg
    17281 ttgcatgtat attctctaaa tctctcaatt aaacaccgcc gcaaagtgta gggagtgaaa
    17341 ttagtgttgt gaaacggtaa tatttgatga ctggtttcaa aaattttgca ggcagactat
    17401 gtggtcagaa tattcactcg aagttgttga tgcggtagcg cgtaatggta gttttagcgc
    17461 tgcggcacag gagttgcatc gcgttccttc tgcggtcagc tataccgtgc gtcagctgga
    17521 agagtggctg gcggtgccgc tgtttgaacg acgtcaccgt gatgtggaac tgaccgctgc
    17581 cggggcgtgg tttctcaaag aagggcgctc tgttgtcaaa aaaatgcaga tcacccggca
    17641 gcaatgtcag cagatagcga acggctggcg cggtcagtta gctatcgcag tggataatat
    17701 tgtcaggcca gaacgtacac ggcagatgat cgttgatttt tatcgccatt ttgatgatgt
    17761 cgaacttctt gtctttcagg aagtgttcaa cggtgtctgg gatgcgcttt ccgacgggcg
    17821 cgtggaactg gcgattggcg caacacgcgc gattccggta ggcggtcgtt atgccttccg
    17881 cgatatgggg atgctaagct ggagttgcgt tgttgccagc caccacccgc tggcgttgat
    17941 ggatggcccg ttcagcgatg atacgttgcg caactggccg tcgttggtgc gcgaagacac
    18001 ctcgcgaacg ctacctaaac gtattacctg gttgctggat aaccaaaaaa gacttgtcgt
    18061 gcctgactgg gaatcatcgg caacctgtat ctcggcagga ttatgcatag ggatggtgcc
    18121 aacacatttc gccaaaccgt ggcttaatga ggggaagtgg gtagcactag aactggaaaa
    18181 ccccttcccg gattcggcat gttgtctgac atggcagcaa aatgatatgt cgcctgcgtt
    18241 aacctggttg cttgagtatc tcggcgatag tgagacgctg aataaggagt ggctgcggga
    18301 gccggaagag actcccgcaa cgggtgatta acgacgatag tcgcggaacg ggccgtcagc
    18361 cacggagcgg cgttcaatca agcgcggatg cacttcaatg gactgcggtt cttcgcgttt
    18421 attgacgata cgatccaaca gcatgttgaa cgccgtttcg cccagcgaat cttttggctg
    18481 atggatcgtg gtcagcgctg gcgtaaaata gcgggcgttg cgcacgttat cataaccgat
    18541 cagcgaaaca tcctgcggca cgcgcagacc catttcatca gcagcacaaa gtgcgcccat
    18601 tgccatgata tcgccaccac agaagacggc agtagggcga tgcggttgcg acagtatttg
    18661 ctgcatggcg cgataaccgg attcaggttc aaagtcaccc tgtacaatcc agctttccgg
    18721 cactttgatc atcgcttctt ccatcgcctt cataaaaccg gcaaggcggc ctgcgccggt
    18781 gttacgttcc agcgggccag ggataacgcc gatttcgcga tgaccgcgtt caatcagata
    18841 acgcccggcc atgtagccgc cttcgaatgc gttatcaatg accgcatcgg tgaagtcagc
    18901 ttttgcttca ccccagtcca tcaccaccat tgggatatgg cgatactctt ccagcatcgc
    18961 cagcaacggc tctgggtact cagaacacat caccagcaga ccatcgacgc gtttttgcgc
    19021 catcatcgac agataagccc gctgtttctc aagattgttc cacgcattgc ccagaatcag
    19081 ggtgtaacct ttctggaagc aatttttttc aaccgcttca atgatctcgg caaaataggc
    19141 cgcttcgctg ctggtcgcca gcaaaccgat agacttggtg tggttaacct tcaggctacg
    19201 cgccaccgcg ctaggggagt agtgtaattc tttaatcgct gcccacacgg cgttgcgcgt
    19261 ttcttcagcg acgaaacgtg ttttgttgat cacgtgtgac acagttgtag tggaaacgtt
    19321 tgctcgtttc gctacatctt ttattgttgc cattccattt cactccagac cctaacttca
    19381 gctcctgcaa aatcgcaagg taaacgtttg cctttacaca ccttacgcac aacaaaagtg
    19441 ttgcggtacg ccggaaaaac gacacggagc gtcaggaagg ggtcaatggc cagtacgcaa
    19501 atagtgtaag cgtggaattt tggccgatct tgacgaaaag gggaagtggt aaaaacactt
    19561 atcattataa atgacgggag attttttcat cacagtgtgt aaaaatgcgt aatatcactt
    19621 tttgtgtggt aataaaaaag gagaaaactt gatgagcacc gaccttaaat tttcactggt
    19681 aacaacgatt atcgtcctcg gtttgatcgt ggtcgtgggt ttgactgccg cgctgcactg
    19741 atttcctctc gagggagcgc gttctccctc gcatctcctt cctgtttgtt agcaaaatgg
    19801 caaaagtgtt ttctcttttt gttattttca tttttttgtg attgctgtca tgctttcgcc
    19861 actttaatga atatcgcgta atgacgcgaa gaggtggaga taaggcatga aaattaacta
    19921 tccgttgctg gcgctggcga ttggcgcgtt tggtatcggg acaacggagt tctcgccaat
    19981 gggcttgttg cccgtcattg cgcgcggtgt ggatgtctcg attcccgctg ccggaatgtt
    20041 aatcagtgct tatgcagttg gcgtaatggt tggcgcgccg ctgatgacgc ttctactttc
    20101 tcatcgtgcc cgccgcagtg cgttgatttt cctgatggcg attttcacgc ttggcaacgt
    20161 actttccgcc atcgcgccgg attatatgac cctgatgctt tcacgcattt tgaccagcct
    20221 gaatcacggg gcattttttg gtttgggttc agtcgtggcc gcaagcgtgg tgccaaaaca
    20281 taaacaggcc agcgcagttg ccactatgtt tatggggtta accctggcaa atatcggtgg
    20341 cgtgccggcg gcgacctggt tgggtgaaac catcggctgg cggatgtcat ttctggcaac
    20401 ggcggggctg ggagtgattt caatggtaag tctgttcttc tcattaccta aaggtggtgc
    20461 aggggcacga cctgaagtga aaaaagagct ggcggtatta atgcgtccgc aggtgctgtc
    20521 tgcattgctg acgacggtac tgggagctgg tgcaatgttt accctctaca cctatatctc
    20581 tccggtactg caaagtatta cccacgcaac accggtgttc gtcacagcaa tgctggtgct
    20641 gattggtgtc ggattctcta tcggtaacta tctcggcggc aaactggcag atcgttcagt
    20701 taacggcacg ttgaaaggct ttttgttgct gctgatggtg attatgctgg caatcccgtt
    20761 cctggcccgc aatgagttcg gcgcagctat tagcatggtg gtgtggggcg ctgcaacctt
    20821 tgcggtcgta ccgccgttac agatgcgcgt gatgcgtgtc gccagtgaag cgccaggtct
    20881 gtcttcatca gtcaatattg gtgcctttaa tcttggaaat gcgctgggag cagctgctgg
    20941 tggtgcggta atttccgctg ggctgggata cagctttgtg ccggtgatgg gggcgattgt
    21001 cgcgggactg gcattattgc tggtgtttat gtcagccaga aaacaacctg aaacagtttg
    21061 cgttgccaac agctaattaa acaaaaacgc agaaggataa ccttctgcgt tacagttgct
    21121 ggcaacgtgc gatttatctt gccgaatgcg ctgttaacgc ctcatccgtg ttggcatggc
    21181 gcgcgaagcg catcaggtaa tgttgcattt gccatcagtt attatgcagc gagatttttc
    21241 gctacgaatt cccagttcac cagcgcccag aagtgctcca gatagccagg acgtgcattg
    21301 cgatagtcga tgtaataagc gtgttcccag acatcaacgg tcagcagcgg agttgcatcg
    21361 gtggtcagtg gagtacccgc gttagaggtt gaaacgatag ccagtttgcc atcgctgttt
    21421 ttcaccagcc aggtccagcc agaaccaaag tttttgatcg ctgcatcagt aaactgagct
    21481 ttgaaatcgg caaagctgcc aaaagatgcg gcgatagctt cagcgacttt tccagtcggt
    21541 tcgccacccg cgttcggtgc caggcagttc cagtagaaag tatggttcca gacctgagct
    21601 gcgttgttga atacgccacc ttcagagctg cgaataatct cttccagtga tttaccttca
    21661 aacgcggtac ctttaatcag gttgttcagg ttagtgacat aagtctgatg atgcttgcca
    21721 tagtgatact cgatggtttc cgcagaaatg tgcggtgcca gagcatcttt agcatatggt
    21781 agtgcaggta attcgaatga cattgctact ctcctttatt attaatttgc atacgtacaa
    21841 tagccttatt gtgcgtatgg atagggtagc aaattaaaag gtgagacaaa agatgaactt
    21901 accctgttgc cgtaacaaca gggtaaagtt ttagcgaagt gtttttgggg tcattacccg
    21961 acgagcgcca acatagtggc gctgccagta gtcttcactg agagaagtga tttgaatttc
    22021 ttggcccgtg cgcggtgact gaataaattt gccgttgccg acatacacgc cgacatgatc
    22081 ggctgtaccg cgtccctgag tacggaagaa gaccaggtcg ccgtttttca gttcactacg
    22141 ttcgataggt gctgcatcac gcaggtgata catttcattc gccgtacgcg gaatacgaat
    22201 tttcaccaaa tctttataag cgtaataaac caggccgctg caatcaaaac cggtacgcgg
    22261 tgagctgccg ccccaacgat atggcttacc aatttgttgc atcagtttat tcattgccac
    22321 tttcgtggct ttttgcacct tcgctttgtg cgcgtcggca agggttcctg acgcggcatt
    22381 tttgactttc acacaatgcg atttatagcc cttacgtttg gtacactttt cggtaaagtt
    22441 ggctgaggcc gttttattga cggcatgttt gctgcgagtt ttggcgtttt tagaagcggt
    22501 aatggaagat tttttaacgg aagaagcggt tttgcttttg gtcgtacttt tactggcggc
    22561 ttttttcgct gttttctggc tggctttctg ggtctttttg gtggttgccg tgcttttctt
    22621 tttatctgct tttttggtga tatgagtagt agcagaactc tccctggctt gctttgaagc
    22681 atgggccata ggcgttaaag gcagggtggt aaaaagtaaa gcacagagcg tgatcgaaat
    22741 acggtttatc cgcgccactg agcgtccctc tgttaaggca ggtctatcat gcctgcgtct
    22801 gatttacaaa acttgatgat tctaatgcat agaagaagag aaagttaata gcttttattg
    22861 ttaaaaaata cgtttcgttg gcgagcgcaa aaaaatgcag cagtgaaagc taaaaatggt
    22921 tataaatacg ccagtttgcc gtgcgtcagg gaagaaacga atatttgaaa tgcaacaaat
    22981 agagcaaagg gataaaatag caaaagcgtg atgaaaatgt tattttccga tgccgcactg
    23041 acccgctaca atgtcagatt actgccgtaa agaagtttga ggaagcaaga caatgagcac
    23101 cactatcgaa aaaatccaac gccagattgc tgaaaacccg atcctgctgt acatgaaagg
    23161 ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca gtccaggcgc ttgccgcatg
    23221 tggcgaacgt tttgcctatg ttgatatcct gcagaatccg gacattcgtg cggaactgcc
    23281 gaaatatgct aactggccga ccttcccgca actgtgggtt gacggcgagc tggtcggcgg
    23341 ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga tcaaagaaac
    23401 tgccgctaaa tacaagtctg aagagccgga cgctgaataa tccgtcctgc aatactgacg
    23461 gatgcgaacg ggcattcgtc agtcctttct ctatccccaa tccagccctt gtggcgaact
    23521 tgaaaatccc gcctcacgca gcagcgtaaa catcggcgtt tgccggaccg gtaggtcatt
    23581 cacttctgtt agcgtaaagc gcagccgtgg ttcgcgacgc agtgcggtag tcagcgcgtg
    23641 gaaaatctct ggggcgagta attcctcttt ttcctgccag accagcattt ttttgccacc
    23701 ttgcgccaga tagagtaaca atttgccgcc agaaacgacc accagcgcac ccgcccgacg
    23761 cgttggtatc agcgttgccg gatgtgcagg ccagggcaga agatttcccc atgcattagc
    23821 cggatcgttg gcagagagcg ccactggtgt atagtggcgc gtttgcgtgg cttgtgtcgc
    23881 cagatcgcgc aatcggtcaa tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc
    23941 tacaaaacga cctcgcataa ttcgcccgga gtcttccata cttcgacaaa gcgtttgcat
    24001 cgatggaaac ccgccaggga tattttcggc tatcaccgcc tgacgactga tgatgccgta
    24061 gcggtcgagc atattttccg ccagcgccag catcctttcg gtatcgttta gtggctccac
    24121 ctgcaataac gaccagcgtc cagcaagctt tggtgtgttg taagatacca gcggcgagac
    24181 gggttgcgca tagacaggac gtccacgacg agcccggtga ctgcggcgag ttgaggtgcg
    24241 tgtgttggta ctgctgcggg tgagggcgcg taacggtgcc caaatgtcgc tggtgatgac
    24301 gccttgccag accagcgccc ataacgcctc ttgcatggct gagggatcaa ccgattcgcc
    24361 gattttatcg cgtatccgtt ggctgatttg ttgtgcaaac caggctcctc cgtcagccag
    24421 aaccgtgact atcgcttgct gcagcgccga actattcgcc tgattcgctt cggcgggagt
    24481 gaacgattct gcggcatatt cctgtagatg cagtgccacc agaccgtcat cttcacccag
    24541 ctttttttgc cccgaccaga taaccgcacc ggttgccagt aattcatcga gcatttctgg
    24601 cgaatagtcg cgtacgcggg caggcaggat ctggctttcc cagagtgagg cgggtaaacc
    24661 gactccggca agctgttcga tcacccgcat gacgccatcc acgccctcat aaacgcctgg
    24721 cgatgttgag gcaaagagtg ccgggctacc atcggtggcg ggtaatacgc cctgacgttc
    24781 cagcagcaat cgcgcatagg tcgtggccgc aacgggacgc gtcgcttctc tggcggcttg
    24841 cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc acccagatgt cttgttgcag
    24901 attcatcacc agcccctgtt cgcgcagttg ctgaagttgc tcttcgacaa tggcaatacc
    24961 gagactaaac tcatgagcca gttgttcaga cgtgaccaga gcatgagccc ggagatagcg
    25021 cagaaagagg tcgcgaagcg ggtaactgac tcggtgcaaa taaatctctg gcagtgactc
    25081 tggtagtcgt acgccgagag catcgcgcag cctggcggca tcatccatac aggcaagacg
    25141 ctcctgcccg ctaatcatgg tcgggaagat tcgttttact gcaagaagat tttccagata
    25201 cgacgcaatc tcttcactgc tgcctgtatg tcgttgcgcc aggtcttcaa cggtcattgg
    25261 ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc
    25321 cagtcgttgc aactcttctt ccacctggcg aatgacctgt gggtcgagta attcccccgg
    25381 atcgacctgt ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta cggatgcccg
    25441 gcgctctgcc agcggggcgt cgctctggta cataaattcc gcgacatagc cgaacaataa
    25501 actggcggcg aaaggcgaag gcgtagtggt cgttacatcg gatatttgaa tttcgccacc
    25561 gttcaggcga cgcatcaaac gttccagcgc gggaagatca taaacatctt gcagacattc
    25621 gcgtagggtt tcgagaatga ccggaaaatc tggatatccc tgagcgattt ccagcaactg
    25681 actggcgcgc agccgttgtt gccagagcgg ggtgcgatgg cccggcgtgc ggcccggcat
    25741 taataatgtc cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg agctgcctac
    25801 cgcctcgcgg acaatttgca gcaacttttc tggttcaaac aaaaaaatcg cggcatcagg
    25861 caatttacca tcggtgtcag gaatgcgcgc aacaatgccg tcatcactgg caaccacaga
    25921 cgcgtcagcg ccccatagtg catgtattcg cccggcaatc gccagcgccc agggttcatg
    25981 cacccgtctt ccatagggag agtgcaaaat aatacgccag tcaccaattt catcacggca
    26041 ccgttcgagg accagatgac gactccccgg aacgatgccc gtcgcgttgc gctgctcgtc
    26101 aatcaacccc tgaatattgg cgatcgtatt ttcttctgcc aaccacgggg gaatagtccc
    26161 ggaaaagaac gcgccatccg ccagtaaatg gagaaaatcg ccgatcatct cgcctaattc
    26221 tgccggacgt ccgttacctt caccacgcca gaagggaagg cgggcagaac gacctggagc
    26281 aggagtcaca atcacctgat cgcgggtgat ttgctggatc cgccatgagg tagcgccgag
    26341 agtgataatg tcgttcaccc gcgactcata taccatctcc tcatccagtt cacccacccg
    26401 ccgcgaaccg gcctgttctt caccttcggg taataacacg ctatacatgc cacgatccgg
    26461 aatggtgcca ccgctggtaa ccgccaacag ctgagcgcca ggtcgggcgg tcaatatccc
    26521 ggtctccctg ttccagacca gttttggtcg aaaagcagaa aaatcgccag agggatagcg
    26581 cccggaaagc atatccagcg tgacgtcaaa aacacgtctt ggcagatctt tccacggtgc
    26641 ggcacggcgt acgcgggagt accattcgtc tgcctgtaat gcatccatcg ccgcggcggc
    26701 aacggtttgc tgcgcaagga cgtcgagtgg attatgcggc ggtgtcaggt tttccagcct
    26761 gcctgcgaac atacactcta caatgactgc ggaatcgact aagtcacgcc tggttcgggg
    26821 gaaaaaaagc cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa
    26881 cccactggca acagaaagcg gcgttgccac ctgaatcacc agatccaccg cgcccatatc
    26941 aatccccagt tcgagactgg aggttgcgac cacacagcgt aattcacccg atttcaacgc
    27001 ctgttcggtg attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa
    27061 aatgtcgcta ctttgtacac ggttagaggt tgcgccggag gtcgactcga aatgggccgc
    27121 atcaacggcg atagatgggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc
    27181 cgtcagtttt tccgccagcc cgcgtgaatt agtaaagaca atggtcgagc gatggcgcaa
    27241 aacttcatca aggatacccg tttcaatata tggccagatg gagccttccc ggccggcatg
    27301 gctgtcttcg ccggtgccgc tggcgaccga tgagacatca tccatattgg cgaccggtac
    27361 gacaatccgt atctgcggat ggcgcattgc gggcgggttg actaccgtaa ccgggcgatc
    27421 gccaccaaga aatgctgcca catcgctggc tgaacgcaca gtggcagaaa ggccaattcg
    27481 ctgtgctgag gtgtggagca gcgtatcgag ccgctccata cttaacgcca gatgcgcacc
    27541 acgtttactg cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgcgtag
    27601 cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat
    27661 atccggcgga ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat
    27721 ccccacgcga agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctg
    27781 taacgggatc tgcaagttgc gctgaacgtc ggtgcccaga gctttgatcg gtgaaatata
    27841 gaggatgcgt gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa
    27901 gagctgatcg agggcgtaga gaaatgctgc cagcgttttc ccggagccag tcggtgcaat
    27961 caccagcgca tgttcgcctc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg
    28021 ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcagggg
    28081 cgatgaaggg tttagattat ctgccattaa cgctattccc cgtgaatatc atgcatgatg
    28141 cgtggaatag cctcactata gatgcctgaa tcattgcatg tcatcaggca tcgactcgat
    28201 tacacctctt cggcggcagg tagcggccag cctcccagac gtttccagcg gttgacgatt
    28261 tcacaaaaca gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg
    28321 tcgaagtcca tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc
    28381 ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagtgag
    28441 gcgcgttctg cggcggccat cataaagctg tgatcgaaat tggcgttgtg cgccaccata
    28501 atggcgcggt tacaaccgct cgctttaata cctttacgca caactttaaa aatttcgtgc
    28561 agcgcctcgt attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaag
    28621 gcgagggcct ctggctgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg
    28681 gtgtccggca tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc
    28741 agcgcatcgg ttttggcgtt aaatccagct gtttcaacat cgatcacaac aggataaaaa
    28801 ccacgaaaac ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa
    28861 tacggataaa ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg
    28921 ggcgcgatga gttcacgccc ggcaggagat tagttgccca gaccgcgacc ggcgtctttc
    28981 tcttcgatta actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagtg
    29041 ccgcctttta ccggacccgc ttcacgggtg acgttaccgc cgttctgacg gattttttcg
    29101 cacgcttcag cggcgttatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg
    29161 tatttatcca cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg
    29221 tagccaacaa acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc
    29281 atgcccagta ctttggtata aaaatcgatg gagcgttgca gatcgccaac gcgcagcatg
    29341 gtatgaagaa gacgcatctt tatatcctca aaatggctaa cagattcaaa atgttgtttt
    29401 agtatagcgg cggctttgcg ccgctcgcaa tgttggatta cagcgtcggg taatcagtgt
    29461 agccttccgc gccgccaccg taaaaacttt cggcacgctg tgggttaagc tcagctttgc
    29521 gttgcaagcg ggcgaccaga tccgggttcg caatccagtc acgaccaaat gccaccgcat
    29581 caattaaccc tttgccgatc agcgtttcgg ctttttctac cgtgtatgca cctgcgccga
    29641 taatcggacc gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta tacggttcac
    29701 cccccgccca atctggttct gacatatgca gataagcaat gccgcgttta cccagttgtt
    29761 ctatcagata cagtgcatcg gcttcttcat tcgggccgtt atctgtgttc tggaaagtac
    29821 cgattggcga aacgcgaatg ccaatgcgat cggcacccca ttcttcaatc ccggcatcga
    29881 ccacttccag caccagacgt gcgcgatttt ccacgctgcc gccgtactga tcggtacgat
    29941 ggtttgaaga aggagaaagg aactgatgca gcaaataacc atgagcagag tggagctcta
    30001 ccagatcaaa accggcttca cgcgcgttag caatggcctg acggaaatca ttgacgatac
    30061 ccggaatctc ttccagttca agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac
    30121 cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag tgcggaaggc gctaccggtg
    30181 cctgaccgcc aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac agctgtacgg
    30241 ccatatgacc attttcagca tgaacgccag cggtaatttt tttccatgcg gcaatttgtt
    30301 ccggactatg gatgccaggt gcaccagcat acccttttgc ctgggcagaa atttgcgtgg
    30361 cttcgctaat aatcaaaccg gcactggcac gttggcgata gtattccgcc atcaacgggg
    30421 tagggatgtc acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt
    30481 ttgccgccgt gatcgcgccc actttcagtg gggaatacag tttttcagat gacataaagg
    30541 ctcccggtcg tctggtgata aaaaatgcct gctaaacggc aggcgtcgca ataatggttt
    30601 ttacatgtgc cagcgcgttt tccagtggct cgaaactgcg cgaaattttg gcctgcagat
    30661 tcgcacccag ccatagtgcg taaagcactt gtgcctgttg cagcggttcg ccacaaaagg
    30721 ttaaacaatg gctatcacgg ccattttcca gcgcctgcga gagcagggcg atcacgccgc
    30781 gagcgccttt atccatcgcg ctacgcatat cttctgacag atcgcacact tcggcagaga
    30841 gttttactgt caggcaacca ctgatggttc catgttggca aaactggttc agtgtttgct
    30901 ggtaataagc cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc aactcagtca
    30961 gtcgttggtg atatgtggcg taatggcgct caagcatggc aacgccaaac gcttctttag
    31021 agcgaaagta gtgatagaag gaccctttcg gcacttcagc ggtttttagt aattcgctta
    31081 gccccatccc ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc aggagatgtt
    31141 cgcgagtatc atgttcggtg tgtttgttca tgcgttggag tgtagtagac cagtcggtct
    31201 agtgcaagca aagttgctac tttgggcatt aacagcttca ataatggtaa taatcttatt
    31261 tgaggagggc gaagtggcgg agcaattaga gttctttccc gttcagagcc cgtgccgggg
    31321 aatttgccag tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc gggatgagcg
    31381 ttttaactgg aataaaatga gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca
    31441 gcgattaatg cgcaaattac gcgcaaataa gccagcgtca tcagacgaac cggagcaacc
    31501 atcactcttt tgagcctaat cttgcgtata ctcctctcat atctgataag aggaagcgat
    31561 tatggttcag cgtattacta ttgcgccgca aggcccggag ttttcccgtt ttgtgatggg
    31621 ctactggcga ttgatggact ggaatatgtc cgcccgccag ctggtcagtt ttattgaaga
    31681 gcatctggat ctcggcgtga ccaccgtgga ccatgctgat atttatggtg gctatcagtg
    31741 cgaagcggcg tttggcgagg cgctgaaact ggctcctcac cttcgtgaac ggatggaaat
    31801 cgtcagtaaa tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat
    31861 tactgaccgc gatcacatca ttaagagcgc cgaacagtcg ctaattaatc ttgcgaccga
    31921 ttatctggat ctgctgttaa tccaccgacc agacccgtta atggatgccg atgaagtggc
    31981 ggacgcgttt aaacatctgc atcagagcgg caaagtgcgc cattttggcg tatcgaactt
    32041 tacgcctgcg caatttgccc tgttgcaatc acgtctgccg tttacccttg ccactaatca
    32101 ggtggaaata tccccggtgc atcagccgtt actgctggat ggcacgctcg accaactgca
    32161 acaactgcgt gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga
    32221 tgattatttc cagccgctgc gtgatgaact ggctgtggtg gcggaggagt taaacgcggg
    32281 ctcgattgaa caggtggttt acgcctgggt attacgttta ccgtcgcagc cgctgccaat
    32341 tatcggctca ggtaaaattg agcgtgtacg ggcagctgtc gaagctgaaa cactgaaaat
    32401 gacccgtcaa caatggttcc gtatccgtaa agcggcattg ggttacgacg taccgtaagc
    32461 acttttagga atagccgccg ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc
    32521 acaatgaacg gaggtcctat gaaacgtttt agtctggcca ttctggcgct ggttgttgcg
    32581 accggcgcac aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggtt
    32641 gggcagtcaa ttggtagcgt caccattact gaaaccgata aaggtctgga gttttcgccc
    32701 gatctgaaag cattaccccc cggtgaacat ggcttccata ttcatgccaa aggaagctgc
    32761 cagccagcca ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg gcatcttgat
    32821 ccacaaaata ccggtaaaca tgaagggcca gaaggtgccg ggcatttagg cgatctgcct
    32881 gcactggtcg tcaataatga cggcaaagct accgatgccg tcatcgcacc tcgtctgaaa
    32941 tcactggatg aagtcaaaga caaagcactg atggtccacg ttggcggcga taatatgtcc
    33001 gatcaaccta aaccgctggg cggtggcggt gaacgctatg cctgtggtgt aattaagtaa
    33061 ttaagaggcc agcgtacctt gtggcggtgc ttgctcaagt tgcgaaagcg agcagtacag
    33121 ccgccagaca attgccgcca gctcgcgggc ggcaggttga tgatggcggg caaggctgtc
    33181 gcaaatccgc tgtaattctt caagtgtggc cgccagtgat ttttgctgaa cgccacgctc
    33241 actcatcact ccccgcaaca gtgaaataca gttatcccgt actcgcgata acggatcgga
    33301 acgcgattcc cagtcgcgca attgccagac aacatgagaa cagttcagca gcactacacc
    33361 ccagcgtaat aaccaacggc gagccagcgc atcctggctg ttactcaact gactgacgtg
    33421 atgataagtg agcgattcaa attcgctttc actcagtgtt ggatggcggc ttagttgatc
    33481 gacaaaatcc cggcgcagcg cgcgtatatg gcggcggctt ttgcgagcat ccgatcctgg
    33541 acgcagaatg gcgaacgcta accacgccaa cgcgacgcca acgatttttg ccagattatc
    33601 gttaagaaaa tcagcaaaat catacaccgg gggattagtg acagcgataa acgaacccat
    33661 aaaaacaatc agttgccccc acagtgcggc aaatttgggc atctgcaatt taagaagctg
    33721 cattgtcgcc agcagtggaa agagaaacag taaaaattgc cacagatcgc taatctgcac
    33781 catcaggcca aatttgacca caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag
    33841 cagtgacaac gacttaaacg gtgctgcgac ggcggagtag agtacgcagc taattgctgc
    33901 cagcgttaat gcattggaac cggcatccca ttgcgaagca atactccatg cgccaatcat
    33961 catcaacgta caaaatgtac gcagtccgct ccacatagct tcggcgttat cggtatgacg
    34021 agccaggccg gatgtccgtg ggagtctggt atgatcatcc acaccgcttt gcagatgttg
    34081 taattcctga ctactttgca gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc
    34141 gacgtgccga tagtcggcga cgttggtcgg gcgtagcggg gcgataatac gtgcgacggt
    34201 gtaaacatct gtttgcgaac tggcgagcgc cgtcagcagc tgttcgagaa tttctcgtgt
    34261 ggcacatggc ggtgagggcc agttgagcaa catacgtcgc aggctggaga tgacactggt
    34321 catacgtaat tgctggtgga gcagcgcatt aaggcgtgcg ttttgctggc gaaaacgata
    34381 gtggctccag aaagcctgga tacggagcaa attcatggtc agtatctgtc caatcacccc
    34441 ctcatgtgct gcacgaatgg catcggttgt ttcaggctgc cagagtaaac tggcatgttc
    34501 cagtaatcgg gcgtgcatgt ttttcaatgc ggttaaaagg gctgtagcat cggaactgct
    34561 cggcaggatc atcatcatca tgccgccgca caaaataccg acaatcacct cgcaaacgcg
    34621 cgcctgagcg atatcccaca gctggctggc ctcagtaata ttaaccatcg gaaaggcgat
    34681 gatggcagct gtgtagcccg ccagttgaaa tgcatacgcg acattattcg tgaagtgcgc
    34741 acaggcccag gtacaaaagc caagccacgc cgccatgctc aaaagaaaaa accacggctc
    34801 attgagcgta tgtccggcaa gaagcagtgc cgcaatggct ccgagcaaac tgccagcgat
    34861 gcgtccgagg cttttgctga tgacaccgcc aacggtggga aagctaacca cggcagccga
    34921 ggtcatcgcc caatagggtt catccagatt taaataatag gcaaccgtca gcgccagaca
    34981 catggcaatg gtattgcgta acgcataacg ccattgcgcc agcgtggccc tgaaccaggg
    35041 caaattgcgc aaggaccatg acgatgcgtt cattattgac ctacggcaat actacaggtg
    35101 gtgcccgaca ccagcgtgac atcgcctggg actttatcca gcgcaaagcg gacgggaact
    35161 cgttgggcga ggcgaaccca ggggacgtta ggtttcacat ccggaataag gtttgagtca
    35221 ctttcaacgc tctgatcata aatcgcccga ccgatactgg aaacgtgacc ctgtaacgtt
    35281 ttgttatcac tatagagggt aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg
    35341 gtttcttcaa aataaccaat gacgtaaaac gagtggctgt cgacaagggc aaacagcggt
    35401 ttcccggtgt cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca tccactcacc
    35461 ggggcgcgaa tttccgtttg cgccagttgc cattgtgcct gtttcagcgt cgcctgtgcg
    35521 gcatcaacac tggcctgcat cgctttaaca ttgaggttag cggtatcgag ttcttcggca
    35581 gaaataaaat tttgtgagag atgacgacga cgattcgctt cgttattggc ttttgcgagg
    35641 tcagattgcg ctttcgccaa ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt
    35701 ttatcgatcg tgagcagaag atcccccgcg ttaaccagtt ggttgtcttt tatattcagc
    35761 tcaacaatac gtcccgacac ctgaggggtg atagaaacct gttctgcgcg tattttcccg
    35821 tcgcgtgtcc acggagattg catgtaataa ttccacagcc accatccggc aagaaccgca
    35881 actaccgcta caatgattgt tgagaaatac ttaattgttt taattgacat agttaccacg
    35941 caatcagtat tgccagggaa aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca
    36001 agggatgcca gatgtcaccg gcgtagatcc agccacgaag caggcggtgt acgacaagcc
    36061 agatgacgaa tccaaacgcg aaggctttga aaaacggagg aaagtagacg gacgcaccga
    36121 acaccagatc ttgtaagggc aatcctgttg cattgagcat aaacttcaca aagtagattc
    36181 ctttacgacc gaatatattg cgttagatta ataaatattc tttaagtgcg aaaaatttac
    36241 gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc agcaatacat ttgttttagc
    36301 aatacaattg ctgcacacta ttctaaaagc cgcataatat cttagcaagc taattataag
    36361 gagatgaaat tggaatcgcc actaggttct gatctggcac ggttggtgcg catatggcgt
    36421 gctctgatag accatcgcct gaaaccgctg gagttaacac aaacccattg ggttacgtta
    36481 cacaatatcc atcagttacc tccagaccag tcgcaaattc aactggcaaa agcgattggc
    36541 atcgagcagc catcactggt ccgtactctg gaccaactgg aagaaaaagg gttaatttcg
    36601 cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta aactgacgga aaaggcagag
    36661 ccgctgatca gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat tttacatggc
    36721 atctccgcag aggaactgga acaactgatt aagctcatcg caaaacttga gcataatatc
    36781 attgagttac aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac
    36841 gcaaacttat ttagcgcggg gaaacggtca cctgactgcc attgctggcc agtacgacac
    36901 gttggcccgg agagaaacga gtgttgcctt gtttctgtac caccatgatg gtattaccat
    36961 cgtctttacg aatttccagc tcgacaccct gcgttttgtt cattgcactc tgtacgccct
    37021 gaccggctac gccacctgca acagcgcctg cagcagtagc cagagaacgc ccggttccgc
    37081 caccaacagt attccccagg aaaccaccaa gaacagcacc gccaattgcg ccgataacgt
    37141 tggaatcatc accgccctga atctgtaccg gacgtacgtt aacgatggtg ccatagctga
    37201 cattctgtac ttgtttcgct tcagaagcgg tataaacatc ccctgacagg gtgtcgttat
    37261 taacacaacc gacaagagac agacctacca ttgaaacaac caatacgcgt ttaatcattg
    37321 aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc ctcatggtca aagtatatgg
    37381 catatgtgaa tccatggtta catgttctac caaatatgag caaatcataa tgaaagtagg
    37441 atcaaccaat tataaacaaa acattcatga tgattattga tttgaagata atgttttact
    37501 accaacggta aaaatcggtc attccgcatg gtattcacga aaagtttatg tttgagtggt
    37561 gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat
    37621 gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg acaattgatg aacaccgggt
    37681 cgcacagctg gcaagtttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga
    37741 tatttgccag ggccagcagc ttatactttc tcagtttgga cagcttgata ctcaactcgg
    37801 gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga
    37861 tatcgttgcg atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc
    37921 acacacttta cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt
    37981 cggtgatttt cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc
    38041 gttccatcat gcgctgctgg ctcacccaac cgagcgacga atggtgctca atattggcgg
    38101 catcgccaat ctgtcactgc tcattcctgg gcagccggtt gggggctacg ataccggtcc
    38161 tggtaacatg ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt acgataaaga
    38221 tgccgagtgg gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga
    38281 cccgtatttc tcgcaacccg caccgaaaag caccggacgc gaatacttta actacggctg
    38341 gctggagcgc catttgcgcc attttccggg tgttgatccc cgtgatgtgc aggccacact
    38401 ggcagaactc accgccgtga ccatttctga acaagttttg ttgagcggtg gctgtgaacg
    38461 attgatggta tgtggtggag gaagtcgtaa tccgctactc atggcgcgtc tggcggcatt
    38521 actgccaggc acagaagtca ccaccactga tgccgttggc attagtggcg atgacatgga
    38581 agcattggct ttcgcctggc ttgcctggcg gacgcttgcg ggattaccag gaaatctgcc
    38641 ttccgtcact ggcgcaagcc aggagacggt actcggggct attttccccg ctaacccgtg
    38701 atatcagagt taactgaatt tttcttaccg cgtctgccgt tacacttgga acgttaggga
    38761 gggcgtattg ccctccagac caggaaagtc ttcgggatat gtctatgaaa aaactgttac
    38821 tgatctgttt gccggtcctt ctcaccggct gtagcgcgtt caatcaactg tttgagcgca
    38881 tgcaaaccga tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata
    38941 atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggca
    39001 tttctgcgtc tggtgcacgt tacaccgacg ggatctatgt tttctggtcg aaaggcgagg
    39061 aagcgactgt ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta caaaatccac
    39121 agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag
    39181 aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc
    39241 aaaggcggat tacgccgccg cgatcttccc gccgatccat taaccctttt tgaacgctgg
    39301 ctctctcagg cgtgtgaagc caaactggcg gaccctaccg cgatggtggt cgctaccgtg
    39361 gatgaacatg gtcagcctta tcagcgcatc gttttactca aacattacga cgaaaaaggc
    39421 atggtgtttt acaccaacct cggcagccgt aaagcacatc aaatcgaaaa taatccgcgc
    39481 gttagcctgc tgttcccgtg gcataccctt gagcgccagg tgatggtgat cggtaaagcg
    39541 gaacgacttt cgactctcga agtgatgaaa tattttcata gtcgcccacg tgatagccag
    39601 attggtgcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt
    39661 aaattcctgg agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagtttttgg
    39721 ggcggttttc gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg
    39781 catgaccgct ttttgtacca gcgtgaaaat gatgcgtgga agattgatcg tcttgcaccc
    39841 tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct
    39901 atgtctattt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca
    39961 tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag
    40021 cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct
    40081 attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat
    40141 gcctgaaacg cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg
    40201 gtctgattgg cgacccgagc ttcaaagctg ctgagcgtaa gctgaacacc gaagaaactg
    40261 ttcaggaatg ggtggacaaa atccgcaagc aggttgcccc gttcctcgat ttcgactgtg
    40321 gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga
    40381 ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg
    40441 ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc
    40501 tgttgcaggg ttatgacttc gcctgtctga acaaacagta cggtgtggtg ctgcaaattg
    40561 gtggttctga ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc
    40621 agaatcaggt gtttggcctg accgttccgc tgatcaccaa agcagacggc accaaatttg
    40681 gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct
    40741 accagttctg gatcaacaca gcggatgccg acgtttaccg cttcctgaag ttcttcacct
    40801 tcatgagcat tgaagagatc aacgccctgg aagaagaaga taaaaacagc ggtaaagcac
    40861 cgcgcgccca gtatgtgctt gcagaacagg tgacacgtct ggtgcatggt gaagaaggtt
    40921 tgcaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttccttgagt gcgctgagtg
    40981 aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gagaagggcg
    41041 cagacctgat gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta
    41101 aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact
    41161 tctttaaaga agaagatcgt ctgtttggtc gttttacctt attgcgtcgc ggcaaaaaga
    41221 attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact
    41281 ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt
    41341 gtttatggtc atgcgggtaa cagtgcggca gagtttccga tgcgccgcct gggcgcgaac
    41401 gtctggccgc tgaacaccgt tcaattttct aatcacactc aatacggcaa atggactggc
    41461 tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag gcattgccgc cattgataaa
    41521 ttacacacct gtgatgccgt attaagtggc tatctgggtt cggcggagca gggtgaacat
    41581 attctcggta tcgttcgtca ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat
    41641 ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat
    41701 gtgcggcacg gtttgcctgc cagcgatatc attgcgccga atctggttga gctggaaata
    41761 ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg tgaactcatt
    41821 gcgcaagggc cacaaattgt gttggttaaa cacctggcgc gggctggcta cagccgtgac
    41881 cgttttgaaa tgctgctggt taccgccgat gaagcctggc atatcagccg tccgctggtg
    41941 gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg
    42001 aaactgcttc agggggcaac gctgcaggag gctctggaac atgtgaccgc tgcagtatac
    42061 gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt tgctgctcag
    42121 gatcgtattg ccaaaccaga acattatttc agcgcaacaa agctctgaaa tatctcattt
    42181 cggcccacaa cgacctgtgg gccgatggct attactttaa gccttccgct gacagcgcgt
    42241 cttgtacttc cggacgttca gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt
    42301 ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag gcatcagcaa
    42361 ttgtaaatcg ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca
    42421 gcttcttctc cagctgcgcg cgaactgtcg atttgtactc ttccggtgta tcagggcgaa
    42481 acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt
    42541 tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa
    42601 gatactgcat aatcgctacg ccttccgtca gcaaagtacc atcgtccagc agcaatgcag
    42661 gtacctgtcc cttagggtta acggcaaagt aatcgtcacc gttttcgaga cgttttttca
    42721 ttaaatccac actgacgagc gtaaaatcct ttccgctctc acgcagggtg atatgggaag
    42781 cgagagagca ggcacccggt ttgtagaaca atttcatcgg taactccttt tgtctgtggt
    42841 ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct
    42901 tgtaaaagag tttccctgaa ttttacgcta cggctgcttt cgccgctttg tctgcagcgt
    42961 catcctgcgt catgcggtgc agtttcggcg cggtcagcag catcagtact gcaataacgg
    43021 cagtagcgac accaatctgc aagaatacgc gaccatagac ttccagtgac atcagtggat
    43081 cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac
    43141 cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt
    43201 gtgcaaccat tgccagaccc agaccagaga tcatcagttc tccgatactc tgcaggccat
    43261 agcttgcgac cagccagctt acagacacga tacctgcgtc agatgcgaat tttgcaccca
    43321 gcggcagaat caggaacgca ccagaacaca tcaccatacc gattgcaaac ttagttggca
    43381 tcggcagggt atcgcccatc ttgttataga tagcggccag aatcgggcta ccaatgatga
    43441 tccagaacgg gttcagtgcc tgatactgct caggttctac ggccaggccc agaatggtgt
    43501 gttcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga
    43561 agaagataat ggcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac
    43621 ctttcatcgc gaaggcttct ttaccgaaga taaccacgat accaaaggca acaacgccca
    43681 gcgccatacg cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg atcagtgcca
    43741 caacaccaat aatggtcagc agcaggttac ggtagttgat aggctcgaag tctggttttg
    43801 aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc
    43861 ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggggtgg
    43921 cgatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt
    43981 ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa
    44041 acaggccgtt accgaccgca atagccgcca tacccatata aacgataccg gcgtcgtgac
    44101 cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccaagc ataattacgc
    44161 gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca
    44221 gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa
    44281 ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa
    44341 tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag
    44401 ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg
    44461 gtgctttctc gcgttatgcc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac
    44521 ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat
    44581 ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa
    44641 aaaacgtaac ggcagatttt ttcactaccc tgagaggggg atgatggctt aacggcgata
    44701 tgttctgcta tttagcgttt ttcctagtga gatagtccag tttctgaaaa atagccagtg
    44761 taatgttttg taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctagttc
    44821 ttgcaatgtt tttacattgc cacagcatcg tgatcttgat cacgaatgaa tagaaaattt
    44881 cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat tacctgatga
    44941 taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg ataaagaaag
    45001 gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt ctctttgtat
    45061 tcacaaagat cttcaataat acaagagcca cagcgaggct tgcgggcaat acaggtataa
    45121 cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg aaccactttc
    45181 agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt acgattacaa
    45241 acgcggaaaa tgtgcgtgtc gacagcaata gtaggccagc cgaatgcagt gtttaatacg
    45301 acgttggctg ttttacgacc tacaccaggc agggcttcaa gcgcagcacg atcttccgga
    45361 acctcgccat tatgctgttc cagtaagata cggcaggttt tgatgatatt ttctgctttg
    45421 ctgttataaa gcccaatggt tttgatatag gttttcaccc cttcgacgcc cagttcaagc
    45481 atcgctgcag gtgtattagc caccgggtag agtttcgccg tcgccttatt aacactgaca
    45541 tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga actgaaatta
    45601 agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc caggcgtttt
    45661 gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt
    45721 cagcacggcg ctttttcatt ctttcatcaa tcaggtattt ccctgccagc atcagtccca
    45781 ggccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg gtgtggaata
    45841 tctccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg tcaaacaatg
    45901 tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag gttgcgccca
    45961 taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt
    46021 cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc cctaatgatt
    46081 gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc accgaggcga
    46141 tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa
    46201 tggtcaagtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc agagcgttag
    46261 tggcagtgga cgtgaccgcc aatagcggac agaggccgag caactggacc agcgcagagt
    46321 tgtttttcca caacccctga acaataacgt ctttaatttc gctcatgctt tattctccac
    46381 aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg gcgcgtttta
    46441 ccgcatttac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac
    46501 catctttctt caccgcccag tttgcatcat ccgcaccact gattttttta cccgcaaaat
    46561 gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc gtttcgtggt
    46621 gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa
    46681 tcgcaccgga atagccatct ggcgcggttg tttccagaac ggcggctacc ggtttgtcat
    46741 cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca gtgaccagat
    46801 agcaactctg tgccagcgca ttgttatagc gttcggctgg cagcacctga tcaaataacg
    46861 ccttttgttg cagactggcc tgttcagcaa ttgtcgtttt ggtcatctgg ttgatggccg
    46921 cagttaaccc tgtagaaccc gctgcaaata gtgccagtgt aatgccgtgt tttcggatag
    46981 ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga
    47041 tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca ggatagccgc
    47101 cgaaactgcg gatcaaccag actaataaac ccgccagcgc gccgaacatc agacgaccac
    47161 gattggtcgt agaagcagta accgggtcag tcaaaataaa gaatgcgccg agcatagtcg
    47221 caccagacag cagatgaatt tgcggtgatg ccagtgtttc tggtgagaac aaccagccca
    47281 acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag cgaatcgctt
    47341 tctgccatag caaccacacg ccgccagcca gccaggcgag atttacccat tgccaaccaa
    47401 cgcccgccag cataccgctg tagatcggat attgcataat ctgttcaacc gaatgaccgg
    47461 cacggacaga ggttttaaat gtatccaacg gtgtcgcctg actaatgcca tcaataccta
    47521 agcgtagcgt gttcatatca ccaccactgg tggtatgtcc gctgaaaata acctgtatgg
    47581 cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct
    47641 gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta aacgggtttt
    47701 gccccagacc gccatacaac tgtttagcaa tgatcaccgc aaacaccgta cccagcacga
    47761 ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct gtcagcaatg
    47821 ctgagttatc tttcaacgtt gcggctaccg actgcttgcg tagtttgagt acgagagctt
    47881 cggctaacag agcactgacc gacgccaaca ggatctgaac gagagtaccc caaccaaaaa
    47941 accacagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg
    48001 atgtttggcg ctggttatgg gtataagggg agctagctat tctgaatacc atttagtcct
    48061 cgtttacaac cttttgctgg gcggcttttt tggcctgaac gcgggcaata gccgcagcaa
    48121 ctgccgcttt gcgcggatca atctgttctt ctggctccgc attagcctgt tgctgttcca
    48181 gcttgcgtgc tttggcacgg gcaatagcag cttcgacggc ggctttgcgc ggatcaacct
    48241 gttcttctgg ttctgcgtta gcctgttgct gttccagctt gcgcgctttg gcacgggcaa
    48301 tagccgcttc gacggcagct ttgcgcggat cgacctgttc ttctggttcc gcattagcct
    48361 gttgctgttc cagcttgcat gctttggcac gggcaatagc ggcttcgacg gcggctttgc
    48421 gcggatcgac ctgttgttct ggctcggcat tagcctgttg ctgctccagc ttgcgcgctt
    48481 tggcacgggc aatagcggct tcgacggcgg ctttgcgcgg atcgacctgt tcttctggtt
    48541 ccgcattagc ctgttgctgt tccagcttgc gcgctttggc gcgggcgata gcggcttcaa
    48601 cggcagtttt acgtggatca gcaacggttg ctgcgtcgtt agtttgctgc agttctgcct
    48661 gtttcgctct ggcttgcgct ttacgggctt cccgtgctgc aataattgca ctgttatccg
    48721 ggcgttcgcc cgctttaatc acaataggct gtgtagcctg ggcctgtttc tctttcaccc
    48781 gcgccagagc ggcagcaatc gcatctttat ctttggctgc aggttgaacg gctgcgctct
    48841 tatgtcgttc aaggcgagcc gctttttcgc gctccagacg agcctggcgc gcttcgaaac
    48901 gcgctttggc ttctgcggcg cgcttttctt cctgacgaat agctgcaatt tcagcttttt
    48961 cctgacggaa atattgcacc aggggaatat tgctcgggca aacccacgcg caagccccac
    49021 attcaatgca gtcggcaatg ttatgcgtgg tagctttatc gtgttgctga cctttgctga
    49081 accagtacaa ctgttgcggc aaaagatccg cagggcaggc gtcagcacag gcgctacacc
    49141 ggatgcagct ttgttcttcc tgtggttcgc caagctcatt ggcagaggga gccaacagac
    49201 agttggtaat ctttacgacc gggacatcca gccatggcaa ggtaaagccc attagcgggc
    49261 cgcccataat caccatttga tcggcagagg ggcagaatcc ggcgtcattc aataaatgac
    49321 gcactggcgt ccccagccgt gcccagacgt tgcccgggcg agcgattgct tcgccagtca
    49381 gggttacgac acgctcggta atcggctcgc catcaataac ggcacgtttc actgcataag
    49441 cagtgccgac gttttgcatt aatacgccga tatcggatga acgcccgcca tgtggaacct
    49501 gcttcccggt cagaatgtag gttaattgtt tagcaccgcc agaaggatat ttggttggaa
    49561 tcacccgcag agaaatatcg tgagagtccg ccagcaccgc gcgcagcatg gaaatcgcct
    49621 gcggtttgtt atcttcaatg ccgataagaa tttcgcgtgg ctgcagaata tgcgcaagaa
    49681 tgcgaatacc ctctacgacc tgagccgcgc aatcctgcat caaacggtca tcggcggtaa
    49741 tgtacggctc gcactcagca gcgttgataa tcaacgtttc aatcttatct ccgccaccct
    49801 gcaatttaac gcctgtcggg aatcctgcac cgcccagccc ggcaacacca aactgatgaa
    49861 tgcgctcgat taactcttcg cgactgcgag agcgataatc ggcccagccg tctcgcggga
    49921 tccagcagtc ttcaccatcg gcatcaataa tcacgcttaa ttcagctaac gctgaaggat
    49981 gagccgtaga gtggggcgca atagccgtaa cggtacccga ggtgggcgcg tgaacaggca
    50041 gcattttgcc gcgaccacgg gtaagcggct ggccgcgcaa tactttatcg ccgacgctaa
    50101 cgcacaactc accttcagcg ccaatatgct gtttcagtgg aataacaaaa cgctgcgcca
    50161 gtggtacctg gcgcaggggt gtaccgttgg actgggtttt catctccggt ggatggatgc
    50221 cgccgttgaa atcccagatt ttattttttc tgaatgcaga gaataactta agcatggtgt
    50281 tccacgggaa tgatacgcac gggaatggtg ttcagatccc atttccagga gtcaggtgtt
    50341 tctgcgaccg gttgcaacga gatgcagtgc gtcgggcacg gatcaacaca taaattgcag
    50401 cccgtacaga gatcactcat taccgtatgc atggcacggg tagcgccaac gatggcgtct
    50461 accggacaag cctgaataca tttagtgcag ccaatacagt tattttcatc aataaccgcc
    50521 accatccgcg caggcgttag ctcttgcgct tcgccatcca gcggctgcgg ctcgacatta
    50581 agcaactcgg caatttttag catcacagct tcgccaccag gggcgcaacg gttgattttt
    50641 tcaccgttat agctgatggc ttccgcgtag gggcgacagc cgggataacc gcactgacca
    50701 cactggctct gcggtaagat ttcgtcaatt ttctcaacga ccggatcgtc ttccaccgca
    50761 aaacggcggg aggcataacc cagaatggcg ccaaacgcca ggcccagcag gctcacggcg
    50821 gcaacggcaa tccagatagc attcattaca acttcaccaa accactaaag cccataaagg
    50881 ccagagacat aagacctgcg gtaattaatg caatggcatt accgcgaaaa ggtgccggga
    50941 catcagccac agcaaggcgt tcgcggatgg cggcgaagag caccatcacc agcgagaaac
    51001 cgacagcggc ggaaaaaccg tacagcgccg actgaaagaa attgtgcccg agattgatat
    51061 tcagcaacgc cacgccgagc actgcacagt tggtggtgat aagcggcaaa aaaatcccca
    51121 gcagacggta aagcaccggg ctggttttgc gcaccaccat ctcggtgaac tgcacaacca
    51181 cagcaatcac cagaataaac gccagggtgc gcaggtaaat cagattaagc gggatcaaaa
    51241 tccacgtatc gataagccag gcgcaaatag acgccagcgt catcacaaac gttgtagcca
    51301 gccccatgcc catcgcggtt tccagctttt tggaaacccc cataaacgga cagagaccga
    51361 gaaacttgac cagtacaaag ttattgacca gtacagttcc gacaaacagt aacaggtagt
    51421 cagtcatttt tcggcctgaa ataaaaaagc cgcctattat ccgttaatcc agagcaggcg
    51481 acaacaggtt aactgtaagg ttattacgga ttcacaaacg tcgctttcac ccgcgacgag
    51541 cgtttaaaat aggggacgat caatgcggtt gccaacaaag taaacagtaa ctggcgaacg
    51601 gctatgccat cttccacggg tgaaaaagcg aacgctttta ctgccagcaa tacggaaata
    51661 agcagccaga taatatagtg tttaggaaca caacgccgac gtttgaagaa agcgatggtc
    51721 aaccacagtg tgtaatacca catggcgata gcggtaataa aagaaacgcc ccataagatt
    51781 tgtgtcgtca gtgcttgttc gccgagcgtt tgaaatgttt gcggagaaga taacgcagcg
    51841 gtatacagca acaacgccag cgtcgtactt aacagagcga ctaaaagcca ggccaatggg
    51901 ccaagcaacc agcctccaat acgttgtggc gttgtggtgg tcataggttc tccataatag
    51961 cggcgcaaac aggcaaatct aattttaggg gcgttattat aaggctttta taaccgatcg
    52021 ctactttctt tattggacat agtgccagac ggacttcggt acctggccga ggtcaaacaa
    52081 acgcccgcca gaaaccagct ctgcgcgacg gtgatcggca gcacgataca tattgatcag
    52141 ttcctgatcg tccgtcaggg tgtaattaag atggtcgtag agtttttcca aactttcgag
    52201 tgaactgatt ttgcgaaatt ttaataagta gtcctgaaca gtcataaaag gtccataaaa
    52261 taattaatac caaaggggta ggtattatta gtgatgataa aatttcacca aaagtgaacg
    52321 cttgtgttat tacaataata agaatgcgct gatggcaaat attcagggat gaaaaactgc
    52381 tctgaaaaga gtaacgcgct ttactatctc tgacaatcac caacaacatc gaacaagata
    52441 ataaattcct ggtttaatat ccgacaagtg aaaacatgca cccggacggg cagcatgtcg
    52501 ctctacaagc gcagagctta cttgtgttgt accgtagtac tctgttcagg tggctgatag
    52561 ttgtcaatgt gactcgccac gccaagaaga atgactgaaa cgacaagaac gatccaacct
    52621 gttaattcaa taagacgatt cattacagcc cacatctctg ttgattgatc cattaacttc
    52681 agggggtaaa tgttacttag caataatagc tccagcaaga tttttactga ggtttttgcg
    52741 atatttagct tttgtcgttg gaaaattcgc tatttttttg acttaagtta aacaacatcc
    52801 cttattgctg gcaggttatt aaactgttga gcgagggtaa aggatagtgt caaatagcca
    52861 tcatacttca atgagaggca atgacatgag cgacaacatc cgtgttgggt tgattgggta
    52921 tggttatgcg agcaaaacct tccatgcgcc cctgattgcg ggcacgcccg ggctggaact
    52981 ggcggtaatc tccagcagcg atgaaacaaa agtaaaagcc gactggccaa cggttacggt
    53041 tgtctctgag ccgaagcatc tgtttaacga tcccaacata gacctgattg tcattcctac
    53101 acccaacgat acccatttcc cgttagccaa agcggcgctt gaggcgggta aacatgtggt
    53161 cgttgataaa ccctttaccg tgacactgtc acaagcgcga gagctggaag cgctggcaaa
    53221 aagcctaggg cgtgtgctgt ctgtattcca taaccgtcgc tgggatagcg atttcctgac
    53281 gctaaaaggt ttgctcgcgg aaggcgtgct gggtgaagtt gcttactttg agtctcattt
    53341 tgaccgcttc cgtccgcagg ttcgcgatcg ttggcgtgaa cagggcggcc caggcagcgg
    53401 tatctggtac gatttagcac cgcaccttct tgatcaggcc attacgctat ttggtttacc
    53461 ggtcagcatg acggtagatt tggcacagtt acggcccgga gcgcagtcga ccgattattt
    53521 ccacgccatc ttgtcctatc cgcagcggcg agtcatttta cacggtacca tgctggcagc
    53581 tgctgagtca gcacgttata tcgtgcatgg atcccgaggc agttatgtga aatatggcct
    53641 cgatccacag gaagaacgac tgaaaaatgg cgagcgtctg ccgcaggaag gctggggcta
    53701 cgatatgcgt gatggcgtac ttacccgcgt ggaaggtgag gaacgtatcg aagaaacgct
    53761 gttgacagta ccagggaatt atccggctta ctatgcggct attcgtgatg cgttaaatgg
    53821 cgatggtgaa aatccggttc cggcaagtca ggcaatccag gtaatggagt tgattgagct
    53881 gggcatcgaa tccgccaaac atcgcgcgac gctgtgcctt gcgtgaaaag aggatggtgc
    53941 aattagcacc atc
//
LOCUS       DAZGTJ010000023        53862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000023 DAZGTJ010000000
VERSION     DAZGTJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.023"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            431..520
                     /gene="tisB"
                     /locus_tag="ACHK8E_001745"
     CDS             431..520
                     /gene="tisB"
                     /locus_tag="ACHK8E_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP0344027.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(644..718)
                     /gene="ysdE"
                     /locus_tag="ACHK8E_001746"
     CDS             complement(644..718)
                     /gene="ysdE"
                     /locus_tag="ACHK8E_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP0344028.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            800..1984
                     /gene="emrD"
                     /locus_tag="ACHK8E_001747"
     CDS             800..1984
                     /gene="emrD"
                     /locus_tag="ACHK8E_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP0344029.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(1992..2489)
                     /gene="yidF"
                     /locus_tag="ACHK8E_001748"
     CDS             complement(1992..2489)
                     /gene="yidF"
                     /locus_tag="ACHK8E_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0344030.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2486..2848)
                     /gene="yidG"
                     /locus_tag="ACHK8E_001749"
     CDS             complement(2486..2848)
                     /gene="yidG"
                     /locus_tag="ACHK8E_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP0344031.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(2838..3185)
                     /gene="yidH"
                     /locus_tag="ACHK8E_001750"
     CDS             complement(2838..3185)
                     /gene="yidH"
                     /locus_tag="ACHK8E_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP0344032.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(3245..4738)
                     /gene="yidJ"
                     /locus_tag="ACHK8E_001751"
     CDS             complement(3245..4738)
                     /gene="yidJ"
                     /locus_tag="ACHK8E_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0344033.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(4735..6450)
                     /gene="yidK"
                     /locus_tag="ACHK8E_001752"
     CDS             complement(4735..6450)
                     /gene="yidK"
                     /locus_tag="ACHK8E_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP0344034.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            6617..7483
                     /gene="yidL"
                     /locus_tag="ACHK8E_001753"
     CDS             6617..7483
                     /gene="yidL"
                     /locus_tag="ACHK8E_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0344035.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(7573..9234)
                     /gene="yidE"
                     /locus_tag="ACHK8E_001754"
     CDS             complement(7573..9234)
                     /gene="yidE"
                     /locus_tag="ACHK8E_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP0344036.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(9430..9858)
                     /gene="ibpB"
                     /locus_tag="ACHK8E_001755"
     CDS             complement(9430..9858)
                     /gene="ibpB"
                     /locus_tag="ACHK8E_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP0344037.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(9970..10383)
                     /gene="ibpA"
                     /locus_tag="ACHK8E_001756"
     CDS             complement(9970..10383)
                     /gene="ibpA"
                     /locus_tag="ACHK8E_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP0344038.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(10377..10449)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            10688..11020
                     /gene="yidQ"
                     /locus_tag="ACHK8E_001757"
     CDS             10688..11020
                     /gene="yidQ"
                     /locus_tag="ACHK8E_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0344039.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(11022..12236)
                     /gene="yidR"
                     /locus_tag="ACHK8E_001758"
     CDS             complement(11022..12236)
                     /gene="yidR"
                     /locus_tag="ACHK8E_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP0344040.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            12337..13401
                     /gene="cbrA"
                     /locus_tag="ACHK8E_001759"
     CDS             12337..13401
                     /gene="cbrA"
                     /locus_tag="ACHK8E_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP0344041.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(13398..14735)
                     /gene="dgoT"
                     /locus_tag="ACHK8E_001760"
     CDS             complement(13398..14735)
                     /gene="dgoT"
                     /locus_tag="ACHK8E_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0344042.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(14810..15958)
                     /gene="dgoD"
                     /locus_tag="ACHK8E_001761"
     CDS             complement(14810..15958)
                     /gene="dgoD"
                     /locus_tag="ACHK8E_001761"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP0344043.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(15955..16572)
                     /gene="dgoA"
                     /locus_tag="ACHK8E_001762"
     CDS             complement(15955..16572)
                     /gene="dgoA"
                     /locus_tag="ACHK8E_001762"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP0344044.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(16556..17434)
                     /gene="dgoK"
                     /locus_tag="ACHK8E_001763"
     CDS             complement(16556..17434)
                     /gene="dgoK"
                     /locus_tag="ACHK8E_001763"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP0344045.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(17431..18120)
                     /gene="dgoR"
                     /locus_tag="ACHK8E_001764"
     CDS             complement(17431..18120)
                     /gene="dgoR"
                     /locus_tag="ACHK8E_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP0344046.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            18397..19053
                     /locus_tag="ACHK8E_001765"
     CDS             18397..19053
                     /locus_tag="ACHK8E_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344047.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(19097..19909)
                     /gene="yidA"
                     /locus_tag="ACHK8E_001766"
     CDS             complement(19097..19909)
                     /gene="yidA"
                     /locus_tag="ACHK8E_001766"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP0344048.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(20024..20422)
                     /gene="yidB"
                     /locus_tag="ACHK8E_001767"
     CDS             complement(20024..20422)
                     /gene="yidB"
                     /locus_tag="ACHK8E_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP0344049.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            20540..20641
                     /locus_tag="ACHK8E_001768"
                     /pseudo
     CDS             20540..20641
                     /locus_tag="ACHK8E_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20662..23076)
                     /gene="gyrB"
                     /locus_tag="ACHK8E_001769"
     CDS             complement(20662..23076)
                     /gene="gyrB"
                     /locus_tag="ACHK8E_001769"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP0344050.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(23105..24178)
                     /gene="recF"
                     /locus_tag="ACHK8E_001770"
     CDS             complement(23105..24178)
                     /gene="recF"
                     /locus_tag="ACHK8E_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP0344051.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(24178..25278)
                     /gene="dnaN"
                     /locus_tag="ACHK8E_001771"
     CDS             complement(24178..25278)
                     /gene="dnaN"
                     /locus_tag="ACHK8E_001771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP0344052.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(25283..26686)
                     /gene="dnaA"
                     /locus_tag="ACHK8E_001772"
     CDS             complement(25283..26686)
                     /gene="dnaA"
                     /locus_tag="ACHK8E_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP0344053.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            26983..27063
                     /locus_tag="ACHK8E_001773"
     CDS             26983..27063
                     /locus_tag="ACHK8E_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344054.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            27293..27433
                     /gene="rpmH"
                     /locus_tag="ACHK8E_001774"
     CDS             27293..27433
                     /gene="rpmH"
                     /locus_tag="ACHK8E_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP0344055.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            27450..27809
                     /gene="rnpA"
                     /locus_tag="ACHK8E_001775"
     CDS             27450..27809
                     /gene="rnpA"
                     /locus_tag="ACHK8E_001775"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP0344056.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            27773..28030
                     /gene="yidD"
                     /locus_tag="ACHK8E_001776"
     CDS             27773..28030
                     /gene="yidD"
                     /locus_tag="ACHK8E_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP0344057.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            28033..29679
                     /gene="yidC"
                     /locus_tag="ACHK8E_001777"
     CDS             28033..29679
                     /gene="yidC"
                     /locus_tag="ACHK8E_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP0344058.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            29785..31149
                     /gene="mnmE"
                     /locus_tag="ACHK8E_001778"
     CDS             29785..31149
                     /gene="mnmE"
                     /locus_tag="ACHK8E_001778"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP0344059.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            31392..31466
                     /gene="tnaC"
                     /locus_tag="ACHK8E_001779"
     CDS             31392..31466
                     /gene="tnaC"
                     /locus_tag="ACHK8E_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP0344060.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            31687..33102
                     /gene="tnaA"
                     /locus_tag="ACHK8E_001780"
     CDS             31687..33102
                     /gene="tnaA"
                     /locus_tag="ACHK8E_001780"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP0344061.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            33194..34441
                     /gene="tnaB"
                     /locus_tag="ACHK8E_001781"
     CDS             33194..34441
                     /gene="tnaB"
                     /locus_tag="ACHK8E_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP0344062.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            34573..35748
                     /gene="mdtL"
                     /locus_tag="ACHK8E_001782"
     CDS             34573..35748
                     /gene="mdtL"
                     /locus_tag="ACHK8E_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP0344063.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            35723..36682
                     /gene="yidZ"
                     /locus_tag="ACHK8E_001783"
     CDS             35723..36682
                     /gene="yidZ"
                     /locus_tag="ACHK8E_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP0344064.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            36839..37588
                     /gene="yieE"
                     /locus_tag="ACHK8E_001784"
     CDS             36839..37588
                     /gene="yieE"
                     /locus_tag="ACHK8E_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP0344065.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            37610..38176
                     /gene="yieF"
                     /locus_tag="ACHK8E_001785"
                     /gene_synonym="chrR"
     CDS             37610..38176
                     /gene="yieF"
                     /locus_tag="ACHK8E_001785"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP0344066.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(38230..39567)
                     /gene="adeP"
                     /locus_tag="ACHK8E_001786"
     CDS             complement(38230..39567)
                     /gene="adeP"
                     /locus_tag="ACHK8E_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP0344067.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            39734..40399
                     /gene="yieH"
                     /locus_tag="ACHK8E_001787"
     CDS             39734..40399
                     /gene="yieH"
                     /locus_tag="ACHK8E_001787"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP0344068.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            40466..40939
                     /gene="cbrB"
                     /locus_tag="ACHK8E_001788"
     CDS             40466..40939
                     /gene="cbrB"
                     /locus_tag="ACHK8E_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFP0344069.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(41021..41743)
                     /gene="yieK"
                     /locus_tag="ACHK8E_001789"
     CDS             complement(41021..41743)
                     /gene="yieK"
                     /locus_tag="ACHK8E_001789"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0344070.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(41758..42927)
                     /gene="yieL"
                     /locus_tag="ACHK8E_001790"
     CDS             complement(41758..42927)
                     /gene="yieL"
                     /locus_tag="ACHK8E_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0344071.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(42954..44570)
                     /gene="bglH"
                     /locus_tag="ACHK8E_001791"
     CDS             complement(42954..44570)
                     /gene="bglH"
                     /locus_tag="ACHK8E_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP0344072.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(44656..46050)
                     /gene="bglB"
                     /locus_tag="ACHK8E_001792"
     CDS             complement(44656..46050)
                     /gene="bglB"
                     /locus_tag="ACHK8E_001792"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP0344073.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(46069..47943)
                     /gene="bglF"
                     /locus_tag="ACHK8E_001793"
     CDS             complement(46069..47943)
                     /gene="bglF"
                     /locus_tag="ACHK8E_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP0344074.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(48077..48913)
                     /gene="bglG"
                     /locus_tag="ACHK8E_001794"
     CDS             complement(48077..48913)
                     /gene="bglG"
                     /locus_tag="ACHK8E_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP0344075.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(49199..49924)
                     /gene="phoU"
                     /locus_tag="ACHK8E_001795"
     CDS             complement(49199..49924)
                     /gene="phoU"
                     /locus_tag="ACHK8E_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP0344076.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(49939..50712)
                     /gene="pstB"
                     /locus_tag="ACHK8E_001796"
     CDS             complement(49939..50712)
                     /gene="pstB"
                     /locus_tag="ACHK8E_001796"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP0344077.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(50803..51693)
                     /gene="pstA"
                     /locus_tag="ACHK8E_001797"
     CDS             complement(50803..51693)
                     /gene="pstA"
                     /locus_tag="ACHK8E_001797"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP0344078.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(51693..52652)
                     /gene="pstC"
                     /locus_tag="ACHK8E_001798"
     CDS             complement(51693..52652)
                     /gene="pstC"
                     /locus_tag="ACHK8E_001798"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP0344079.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(52739..53779)
                     /gene="pstS"
                     /locus_tag="ACHK8E_001799"
     CDS             complement(52739..53779)
                     /gene="pstS"
                     /locus_tag="ACHK8E_001799"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP0344080.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
BASE COUNT        13182 a        14145 c        13350 g        13185 t
ORIGIN      
        1 aaccccgccg gagcgaggtt tcgtcagtcg cctgcggctg gtaaccgcaa agcacactgt
       61 attatgtcaa cactgaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt
      121 ttgacgtatt tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaaattact
      181 gtttatttat acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg
      241 catcgttacc cggcgcacta agtcctggca gaaacgggtg gtgccgtcag cgccttaacc
      301 ccgcgtgagc acactgtgtt atgtcaacaa gcacaacgtt tctccttgag ataccgcgtg
      361 cacaacagct ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca
      421 ggagacgcgt atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc
      481 actgcaactg cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacta
      541 aaggggagcg ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga
      601 tgctgacgca tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt
      661 tacatattct gccgctaaac aattccccat tcctggcgta tatctggcta acattcatca
      721 atgtgataga ttcctctccc gcatttatgg gaatgcgtag tgacttattc taattatttt
      781 tataaaagca tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt
      841 attactcgtg gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat
      901 ggcgcgcgat ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct
      961 gacttacggt gtctcacagc tgttttatgg cccgatttcc gaccgcgtgg gtcgccgacc
     1021 ggtgatcctc gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc
     1081 cagtttgacg gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg
     1141 cgtaatggcg cgtactttgc cgcgcgattt atatgaacgg acacagttgc gccatgctaa
     1201 cagcctgtta aacatgggga ttcttgtcag tccgttgctc gcaccgctaa tcggcggtct
     1261 gctggatacg atgtggaact ggcgtgcctg ttatctcttt ttgttggttc tttgtgccgg
     1321 tgtgactttc agtatggccc gctggatgcc ggaaacgcgt ccggtcgacg caccgcgcac
     1381 gcgcctgctt accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct
     1441 gatgctgatt ggcggtctgg ccggaattgc cgcctttgaa gcctgctccg gcgtgctgat
     1501 gggcgcggtg ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc
     1561 ggcggcattt tttggcgcat ggtttgccgg acgtcccaat aaacgcttct caacgttgat
     1621 gtggcagtcg gttatctgtt gcctgctggc tggcttgctg atgtggatcc ccgactggtt
     1681 tggcgtgatg aatgtctgga cgctgctcat tcccgccgcg ctgttctttt tcggtgccgg
     1741 gatgctgttt ccactggcta ccagcggcgc gatggagccg ttccccttcc tggcgggcac
     1801 cgctggcgcg ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc
     1861 tgcgatgttg ccgcaaaccg gtcagggcag cctgggattg ttgatgacct taatgggatt
     1921 gttgatcgtg ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcctgt
     1981 ttaagctcac gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc
     2041 agatcacgca tcacccgcat atgcggcgcg gagtaagaat aaaacgcctg atagcccgca
     2101 caaagcacgt tgtctggttt atcttcccga gcgtgcgggc acagccgatg ccagtcgcac
     2161 gcctggcagt cacggcgcgg ttgcgacttt tcgctgccac accatttttg taaggtggtg
     2221 tcaaaaagtt caatcgaaat acgactgata tctgcccgca cccaacactc gaacagggca
     2281 attaaaaatt tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca
     2341 tcaaattcaa gatggggaat aagctgtact gagcggatgc ccagctgttt gaaattgtga
     2401 taaaaatcgg caggttgcag agcatcctta tactctacaa ccagtttatg cctgtcctct
     2461 tcagcatcaa tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat
     2521 gtatatgcgt tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt
     2581 tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat
     2641 tacgggtgta gtgccagagg atcagcgcca cgatggcgag gatgccaatc gaaatccaga
     2701 ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc
     2761 ccagcatggt acgaaaccac gccagtgatg tgcgttccgg ctgcagccct gggtcggcaa
     2821 tgcgtctggc tttgcggcta tccggcatac aacaccagtc ccatgacgat cactgccacg
     2881 accatcaata ttaaactgat gattaataag ctgttggtat agggcaggtc ctctttcagg
     2941 cgcatcgctt tttcattgcg taaccagcgc aagtagccat acatcgctaa cccgccggaa
     3001 aacagacaca acaacaacgc cagcagttcg cggatgacgg gcgtggcgaa atctggcgca
     3061 agttgatcaa gcccaacccc cgccgccaga aatcctagtg cagtacggat ccacgccaga
     3121 aaagtacgct cattagccag cgagaagcgg taatcgggcg cttctccgag gcgggaaatt
     3181 ttcattgttg ctccttgtaa actgttcctc gccgaaaaat taggccagga acagcaataa
     3241 tgcatcagaa cttctgtttt ttctcttcca ccttcacccc ttgtgtcggt aaaccggtgt
     3301 catagtcgcg taccactgga gaataaccat cctgtgggcg tggacgaaac gcccccatcc
     3361 agcgcggttg tgcatctttg cgccacggac gcaggttcca ttgataactg cggaacggat
     3421 cgcgaatttt gtccatataa tccaataagg cgtcatgcat tttgctgcga acgtcggcaa
     3481 aatggatatc atcgatcagg ttatgcattt catttgggtc attacggcga tcgtaaagtt
     3541 catcactggt gaagaggttg agcaccagtt taaagtcatc cgttacccag caacgcaccg
     3601 gaataaaacc gccaaagctg tcatgctcaa tctcgtagcg attaaattcc accaacacgc
     3661 cacgtggttc ttttacggca aggatattat cccccggcag aatctctggt ttttcaatat
     3721 ctgccagcgc catcattgtc ggcagtaagt caatatgact gactggcgta tcgacctgtc
     3781 gccgctcccc ttgcggcgaa cggatgatca gcggaatgcg ggtgatgtcg tcatacatcg
     3841 ccgccccttt actgatcagc ttatgtgcgc ccatcatttc accatgatcg gaggtataaa
     3901 tgacccacgt attttcacgt tgctctggcg ttaaggcatt gatgacacgt ccgatttggt
     3961 catcaacaaa gtcattgcag gcaaaataga gcggatggtg ataaagcccg tcatcaccga
     4021 ctggcgatgg catcgcctgc gcccataagc ggtgatgttc cggtttgttc gccaggtcat
     4081 cctgtgattt ctcgccaagt tcgtagtaaa aatcagcgta tttctctaaa tactccaccg
     4141 gacaggtgaa cgggtgatgc ggctcatcat acgaaaccac catcaggaaa ggttcgtcgg
     4201 cgcgcgcggg ctgttgcaga aaatctaccg cccgattgct gatgcggtgc gcccaggtga
     4261 aggtttcgtc gatatggttc gcctgtaaat cctcaacgct gtttaggcca ttgcgccaca
     4321 ggctgatctc tttttccgtc agttcgctaa ggtagttcgc cccatcgaac cagtaatcag
     4381 cgtcccactc cggcggacac tcgccagtgc cgaaatagtc atgaccgtcg agatgccatt
     4441 tgccaatgta acaggtgtga tagcccgcat ccttaaagta gcgccccatg gtggaaatgt
     4501 ttttacctgg cgcgacgttg ttggtccacg ggccggactg gttagcgtag ataccggtaa
     4561 acagcccggc gcgcgctggt gtacaaaccg gtgaacaggt ataggcggaa ttaaagcgaa
     4621 tactttccgc cgccagacta tcaatatttt gcgtattcag cggcttaccg ctatagcaac
     4681 cgaccatatt ggtggcctgg gtatcggtca tgatgaacag aaaattgggg cgtttcatag
     4741 gctatccttc gcgtcgggag taaaggttac ggctgggtcg tgacgatgcc gccagctatc
     4801 aaaaatcagg tagacaatca ctacggcggc aatgaaataa ctgatcatcg ctaaccagcg
     4861 cgtaccgtag ccgccgaatt cagccagccc ggcatagacg ccaatcatcg cgaacaggat
     4921 gcccattgac gcgatcttga cgtttttcca cggtttcatg tccaccgcaa acgcatcttt
     4981 gaaggtgaac ggcgtggcgc gcggtttgat aaaaccgatc accagcatca cgaccacgtt
     5041 gatgcagaac gtacaggcca gcacatagag gaaatggaag tcgaacttca ccagatagtt
     5101 gatggtgatg tagctgatta tgccaatccc catcgccact tttgccgcca gcgccgggat
     5161 gcgcgggaag aaaaagccca taatgatgat ggtaaccagc ggcacgttgt aaatgccgtt
     5221 gagctgtttc atccagctat acagcccctg cggcgcgttg gcgatccacg gcgctaccag
     5281 caccgaaacg atggcgataa agaaaccaaa tttgcgcccg acggtgacca gctgctgcgg
     5341 ctcggcattc tggttaatga tgcgccggta aatacccata ctgaataacg tactggcgct
     5401 attcagaaag ccgttgaagg tactgatcac cgcaccaaat aacaccgcgc cgaagaaacc
     5461 caccagtggc actggcagaa cgttattgac cagcgtcggg taggccatgt cggctttcgg
     5521 tagatcctga tacagatgaa acgcaatcaa ccctggcagc accagtacca gcgggtcgag
     5581 cattttcagc accgccgtta acagcgcccc cttttgccct tccgccaggc tttttgacgc
     5641 cagcgtgcgt tgcacgatgc cctgatttgt acaccagtaa aaggtgttca ccagaatcaa
     5701 accggtaaat gccgcgccaa tcggcaaggg atcggtcggg ccgccgactg agtttaattt
     5761 ctcggcgtga acggtggtga gttgctcaat gccctgcata aagctgccct tgcccatcgc
     5821 gatcaggcca aataccggca ccatcaaccc gccaataacc agcccaatac cgttaatgga
     5881 gtccgccact gccattgcgc gcagtccgcc gatcaccgca tacaaaattc ccgccagacc
     5941 aagcaaaata accagtaacc agatagccgc accatcggaa agttgtagcg actcacccac
     6001 atgaaacagg ctgttgagcg ccaacgcgcc ggaatagaga acaatcggca gaaagcagac
     6061 gccggtggca atgaggaagc agaagtcgat gataatacgc gtcgttttat cataacgttc
     6121 ctccagaaaa tcggggatgg tggcaatgcc gcgcttcaga tagcgcggta aaaagattag
     6181 cgcgaggaag atcagcgtca ctgcagaggt gacttcccag cccatcaccg acatgccact
     6241 tttgtaggcc tgcccagaaa ggccgaccag ttgttccgtg gaaaggttgg ttagcattaa
     6301 cgaagcggca ataaccggcg cttttagtga acgtccggca agaaaatagc cttgttgcga
     6361 tccggtatct gttttacgga ccttccacca ggtgatgacc gctaccagca gcgtaaaacc
     6421 gacaaaactc aagatttgta acgaattcat ctcatagccc ttatttatgt ttatgtcccg
     6481 gacgcatgaa tatagggaga ggtaaaatgc gtcgggtcag gcagtcagat tcttaaaata
     6541 caaacgtcgt atccctgaac ggattgtttt ctgttaagtt caggttgtga gcataattcg
     6601 ccggagggat attaaaatga atggaaaatt gcaaagttcg gatgtaaaaa acgaaactcc
     6661 gtacaatatt cccttattga ttaacgaaaa tgtgatctcc agcggaattt ctctgatatc
     6721 gctctggcat acctacgccg acgagcatta tcgggtgatc tggccgcggg acaagaagaa
     6781 accgcttatc gccaactcat gggttgcggt gtataccgta caaggatgtg ggaaaattct
     6841 tttaaagaat gacgaacaaa taactctgca tggcaactgt attatatttt taaagccaat
     6901 ggatattcac tcttatcact gtgaaggttt ggtctgggaa cagtattgga tggaatttac
     6961 ccccaccagt atgatggata ttcccgtcgg tcagcaaagc gttatttata atggcgaagt
     7021 ttataatcag gaactcaccg aagttgctga gttaattact tcaccagaag caataaaaaa
     7081 taatctggca gtcgcttttc tgacgaaaat tatttatcag tggatttgtc ttatgcacgc
     7141 agtcggtaaa aaagatccac aacggcggca aattgaaaaa ttaattgcca ctttacatgc
     7201 cagtctgcaa caacgctgga gcgtagctga tatggctgcc acgatcccct gtagcgaggc
     7261 ctggttgcgt cgtctgtttt tacgctatac cggcaagacg ccgaaagaat attacctcga
     7321 tgcgcgtctg gatctggcgc tatcgctatt aaagcaacaa gggaactcgg ttggcgaagt
     7381 cgctgatacg ctcaacttct tcgactcctt tcatttcagc aaagccttta agcataaatt
     7441 tggttatgcg ccgtcagctg tgctgaagaa tacggacagg taataaccag aatggcgagt
     7501 gtgtaaatga ctcgatgatg ttcagaaata ccaggtggag taccatttac ggcgactcca
     7561 cccggaaaag agttaaccga tactccagaa gagcaccgcc agtaattggg gggtgataat
     7621 gcgcaggaac atcaccaacg gatagacagt ggcgtaagag agcgccgccg caccgctcgt
     7681 tggatgaaga ttattagcaa atgccagcgc cggaggatcg gtcatggagc cagccagcat
     7741 cccgcacatg gtcaggtaat tcattttggc taacatccgc gccagaatgc caacagtaat
     7801 cagcggaacg gcggtgatca gggcaccata accaatccag cttagccctt cgccatccac
     7861 cagtgtatgg ataaaatcgc cgccggattt caggccaacc accgagagaa agagcacaat
     7921 gcctaactcg cgtagcgcca ggttagcgct tggtggcata aaccagtaca gcttgccgat
     7981 actgccgata cgcccgagga tcaacgccat aatcagcgga ccgcccgcca gcccgagttt
     8041 caacgccgcc gggaacccag gcacaaagac gggaatagaa cctaacaata cgccaagccc
     8101 gataccaata aacactggca gcatctgaac ctgttgcagt ttttgctgcg cattccccag
     8161 cacattggca acggcatcaa ttgcggacgg acgccccacc aggttgagga tatcgccgaa
     8221 ctgcaggctg atatcgctac ttgcaactaa ttcaacacca gcacggttca ggcgcgagat
     8281 aacaacgtca tagcgttctt taaagtgcag gtcgcgaata cgtttaccga gcacgttttc
     8341 attggtgacc accacacgct cgacgcgcaa gtcagtgcct ttcgtagaca gcgaggtatc
     8401 gacctcctga ccaatcacca gttgcgcatt atgtaaatcc gcaggctgcc ccaccagatg
     8461 cagcaaatcg cccagttgaa taacggtttc tggcgaaggc acttttaggg tttcttctcg
     8521 tttcaatcgt gagcaaataa ctttatcgcc attgagaatc ggcacatctt taatggctaa
     8581 attatgcaaa ttaggattct caactcggat gttaatcgtc ctgattagcg caccgccgtt
     8641 ggttcgcgtg gactcgtgct gctgggcctc agtctcaaca ttgacgcgga aaataacccg
     8701 cagcatccac atagtgaaca aaatgccgca aatcccaaat ggatacgcca tcgcatagct
     8761 catccccatt tgatcgacca tcgccatcgg tgtccccagg tcgcgcaaga tctgctgccc
     8821 tgcccccagt gctggcgtat tggttaccgc accggagaaa atccccagta ctaccggcag
     8881 tggaatatca aacagtttat gcaggatggc ggtaaccagc cccccgatga tgacgatcag
     8941 aacagcaaac aggttgaggc gtaatccgga gacgcgcaat gaggcaaaga agcccggccc
     9001 tacctgaatc ccgatggtat aaacgaacag gatcaggcca aattcctgaa taacatgcag
     9061 catatcgcta cttaatgtca tccccgcctg agaaacaaaa tggccgacga tgatcccacc
     9121 gaacagcacg ccgccaatgc ccaatccaac accgcgaaac ttgacgttgc cgataaacaa
     9181 cccgacgacc gccaccaaag ccagaatact gaccgttaat gctatatcac tcatcgtcca
     9241 tttctcttga gataacattt taattgctac ggattatggc tgaatcggtg aagcagattt
     9301 gccttatagc gcacattatc acgggaaaga acgtgccgaa atatcttaaa caggcgaaaa
     9361 ttaacgcatt gttgaagaag aaagacaaaa aaagccccac cagaatggcg gggcaaagag
     9421 aatagctagt tagctattta acgcgggacg ttcgctgata gcgatacgct gcgctgcgat
     9481 gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa ccgtttacga aggttgcacc
     9541 agagacttcc atattttcag ccagcgtaaa gctcaggcta aatggctgat tcataagccc
     9601 ttgatgcagc cattttttct cttcttttgg ctgctccggc gtgcctttta cgctcaggcg
     9661 cgtaccttcc agttgaatct ctaaatcttc ctgacgaaaa cctgccagcg caagggtaat
     9721 gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc gggaagctct ggctttcacc
     9781 ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc cattgacgca tcagtgggga
     9841 taaatcgaag ttacgcatag tcatttctcc ttctaagaag cgagtaagta cctgcaaatc
     9901 cgaagattcg catatgctcc ctgacggcga gcatggagat gtcaggccgc gcaaggcggc
     9961 cttagggaat tagttgattt cgatacggcg cggttttttc gcttccggaa tcacgcgttc
    10021 gagatcgata tacagcaaac catttaccag gttagcacca cgaacatgaa tgttctcagc
    10081 taactggaat ttgcgttcaa agttgcgttc agcgatgccc tggtacagat aggtgcgctc
    10141 tttttgttcg tcggcgtgag cacctttcac caccagcaga ttatcctggg cggtaatttc
    10201 cagttcgctc tcagcaaaac cagccacagc gatagcaatg cggtaatggt tttcgtctac
    10261 cagttcaacg ttatacggag ggtagccgcc attactctgg ctctggttgt tttctaagtg
    10321 gttaaacaaa cggtcaaatc caatagcaga acggtaaagc ggggataaat caaagttacg
    10381 cataatcaat agctcctgaa atcagcgaga atctaagacc ttccacaatg gacaggtcag
    10441 gtagccagaa cacccatcag gcgcgtcctc atcggctacg atgtaaaaat gggtctggaa
    10501 atgaactttt caagccttat cagcggactt tttttgcagt ttatggtcta ttgcatagac
    10561 tgagggggca gcacgaaacg ttaaagtgcg atagccgcca cagtgcgacg taatgatgat
    10621 gttattttca gcaagggatc gctataactc atcaggccaa cacccacgat caacagatga
    10681 atttattatg ataagaaatg tgttgttagc gttcatgata tgcagcggaa tgacattact
    10741 tggaggatgc tccagcgtga tgtcccatac cggggggaaa gaaggaacgt atcccggcac
    10801 ccgcgccagc gctaccatga ttggcgacga tgaaaccaac tggggcacta agtcgctggc
    10861 gattctcgat atgccgttta ccgctgtgat ggacacgctt ttactgccgt gggacgtgtt
    10921 ccgcaaagac agctcggtgc gctcacgcgt tgaaaaaagc gaagctaacg cgcaagccac
    10981 caatgcggtt attccacccg ccagaatgcc tgataactga tttagcgatc agtttcggtg
    11041 atccacagct ggccgccttc catccacgcc agccactgac catccggcga gaagaccacg
    11101 gcgtccgcag aaggtggatt ggcgtggttt tcggttaaat actcaacctc gccgctttgc
    11161 gcatgggcgc aagcaattcg attatccagc acaaagccca accattctcc tgatggatgc
    11221 cagttaaatg cagactgaat atccgttttg ttatgggtta actggcgcgg ctcgccgccc
    11281 tgtggcgaga taagccacag ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt
    11341 tgcgtaccct gcggattaca gcgcacccag tggcgcggga cgttgactaa ccccggataa
    11401 gcccgatggt gggtaaaggt taaacgtcgc tgcacgacgc cacgcggtgg cgcgggcagg
    11461 gttgtttccg ttccacttaa cggcgcatca ccagctgctt tccagccagc ttcatcttgc
    11521 ggtaactcaa cgataaatag ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca
    11581 ataaacgcca gcgcgtgatt tcctacccat ccttcttcat aagcacgatt gatttcatta
    11641 ctgccaggct gcggcgtggg cgttgtttta ctcaccagca cgcaccagtg gctaccgctg
    11701 tattcacgcg gatgctgctt ttgtacgttg accgggccaa acggcgcagc aacacctacg
    11761 tttcgcaaat ccagcgtcga atcgagttga tgcattacat ggtcgttata ggtaaagctc
    11821 accctttctc cgttcgggct aaagacatgc acatggctgc cgccgcgcag cgctcctggg
    11881 gtgtacggcg cggtaatatc cattgcatcg agattgctca ccttgccgcc ttcagcaatc
    11941 actccgcgcc gatgatggaa gtcgtaatac catgtttcat caggattttc cgggccgtgg
    12001 atgaaaacat atttctctga cttcggatga acggtcacca cgccgacgta cgcgccctgt
    12061 gacgcgcgat agataacctc gacctcgccg gtatggatat tcacacgctc aatggtctcg
    12121 ccggtaaacg acgcccctga aggacgcacg tcaaatacca gccactggct atcgggcgtc
    12181 caggtattgg tattggtgag caggtgatta cggggagcaa aggttatctg tttcatggcg
    12241 cggtcctgat acaaaacggg tcccgccatc atacttcaca aggacttcac tttcagacaa
    12301 tagtcttttg ccatcgtgat gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt
    12361 attggcctcg gcccggcagg gtcggcgttg gcgcgaaagt tagcaggcaa aatgcaggtg
    12421 atcgcgctgg ataaaaagca ccagcatggt actgaaggtt tcagcaaacc ttgtggcggt
    12481 ctgctggcac cggacgcgca gcgatctttt attcgcgatg gactgacgct tcctgtcgat
    12541 gtcatcgcca acccgcagat tttcagcgtc aaaaccgtcg acgtcgccgc atcgctcacg
    12601 cgtaactacc agcgaagcta tatcaatatt aatcgccatg ctttcgactt gtggatgaaa
    12661 tcgctgatcc ccgccagcgt tgaggtttac cacgacagcc tgtgtcgaaa aatctggcgt
    12721 gaggatgata aatggcatgt catttttcgc gcagacggct gggagcagca tattaccgcc
    12781 cgctatctgg tcggtgccga tggtgccaac tcgatggtgc ggcgacatct ctacccggat
    12841 catcaaattc gtaaatatgt cgctatccag cagtggttcg cagagaaaca tccggtgccg
    12901 ttctactcct gcatctttga taatgcgata actgactgtt attcatggag tatcagcaaa
    12961 gacggttatt ttatctttgg cggtgcctat cccatgaaag acggtcagac gcgtttcacg
    13021 atgctgaaag agaaaatgag cgcctttcag ttccagtttg gtaaggcggt gaaaagcgaa
    13081 aaatgcacgg tgctgtttcc ctcacgctgg caggattttg tctgcggtaa ggacaatgcc
    13141 tttctgattg gtgaagcggc gggatttatc agcgccagct cgctggaggg gattagctat
    13201 gcgctggata gcgcagagat tctgcgttcg gtgttactga agcagccgga gaagagcaac
    13261 gccgcctact ggcgcgccac ccgcaaactg cgtttaaaac tcttcggcaa gatagtaaaa
    13321 agccgatgcc tgaccgcacc ggctttaaga aagtggatta tgcgcagtgg tgtggcgcat
    13381 attccacagt tgaaagatta gccaacgcgc ttcacatcgc ccaccagcag gatgtaagag
    13441 agcgcgccaa tcaacgcgac ggcggagata taaaccagtg caggtgcgaa accgtaaccc
    13501 tgcgccaggt accccaccac cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta
    13561 aacacgccgc cggttaaacc aatcaggcgc atcggtgcca gagaagagac cagcgaccag
    13621 gtaatcgaag caaaaccgtt gccgaagaat gccagcgcca tcaggcacat aatcatcatc
    13681 ggatcgttgg tgtagttagc gcccataatg caggtggaga tcagcaagcc gcagataatc
    13741 ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta ccagcagatc cgcgacccag
    13801 ccagagagca ggacgccgac aaacgccgcg aggaatggca ccgtggtcat aaagcctgct
    13861 ttcagcgccg tgattccttt ttcctgggtt aaatagttcg ggaaccaagt taagaaaaac
    13921 cacagtgtag aagccaccgc aaattgccca agatagacgc cgatcagttt acgatggaac
    13981 accagtttcc agtctttggc tgttaacggc tgacgcgcct cttttttcac cggcgcatca
    14041 ccatccacca gaccgccgcc atcacgaatg taatccagtt cagctttgct gatgccttta
    14101 gtcaggcgcg gcggctgata aaccttaaac caaatcagcg accagataat gccgatacca
    14161 ccggtgacaa tgaacaccca gtgccagctc aacatttcct gaatccagat cagcagcgga
    14221 gtcaaaaacg ccagaccgac aaactgacca gacgtataaa aaccaacggc ggaagcgcgt
    14281 tcatgttccg ggaaccagct ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg
    14341 aaaataccgg ttattgcgcg cagaccaatt aatgacatca atcccgtggc aaagccctgg
    14401 aataaagtcg ccactgacca gccaaatatc gcaataaaat aagtcacgcg tgaacctacg
    14461 cgatctaaaa accaaccgcc ggggatctga catagtgtat aaagccaggc gaaggccgaa
    14521 aatacatagc ccatttccgc tttggtaata ccgaactctt cctgaatatg ggcggaagcc
    14581 acggccaggt tagcgcggtc aacataacaa atgaccaccg taataaagat catcaccagc
    14641 gtcagataac gccgacgccc cggctttgct gcattaacgg gaatatccat agcgagcttt
    14701 ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca gagggttata
    14761 tttttgtatt gctgttttag tgcccgatga gaggcttacg tggcaggaat taccactctg
    14821 ctacgctgtt atcttcatga cgccagagcg gattacgcca gtccggggca tttttactga
    14881 actcaataac tttagcttcg tcgatttcca cacctaagcc cggtttcgtt aatggtttaa
    14941 agaaaccgcc gaccatgctg aagtcttctt tgtttttcac aaagtcgagt aactccgcgc
    15001 ctttgttgta atgaatgccc atactttgtt cctgaagtac ggcgttatag gaaacaaagt
    15061 cgatatgcag gcaagccgcc agtgcaatcg gtccgagcgg acagtgcggc gcaagggtca
    15121 cgtcataggc ttctgccatt ccggcaattt tgtagcattc ggtaataccg cccgcgtggg
    15181 agagatctgg ttgcagaatc gaaataccac ccgcctccag cacacgttta aaatcgaaac
    15241 gtgagaacat gcgttcaccc gccgccagtg gaatatgcgt ttgtgccgcc agtttcgggt
    15301 agtattcggc ttgttctgcc agcaccggct cttcaataaa cagcgggcga tacggctcca
    15361 gttctttaat cagcactttc gccatcggcg cgctgacgcg accgtggaaa tcaagaccaa
    15421 actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttgact gccgcatcta
    15481 ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagtttg aaggtatcga
    15541 agccgatttc gcgcagggtt ttaatgccgt cgataacatc cgccggacga tcgccgccga
    15601 cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc catcagttgc cagaccggcg
    15661 cattcagcac tttgcctttg atatcccata acgcctggtc gatcccggcg atggcgctca
    15721 tcaggattgg accgccacga taaaatccgg cgcgatacat cacttgccat aagtcattga
    15781 tgcgcgatgg atcctgacca atcaaatagt cacccagctc gtgaactgcc gcttccaccg
    15841 tgcgggcgcg gccttcaatc actggctcgc cccagccgac cacgccttca tcggtttcaa
    15901 ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg attttcattg
    15961 cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga
    16021 ctgcccggcg cgatagagat cgctgcctaa gcccgcccct gcacaacctg cgtctatcca
    16081 ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt ccggtggtaa
    16141 taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa atattttcag
    16201 cgcctgagcg cccgcgtcga gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca
    16261 aacggtcatg ccgtagccca ccgcacggcg gatcacttca ctatggatat tgggcgtaac
    16321 gatgagctgg cagctcatcc tggcgagtgc atcgacctgt tcaggtttca gtaccgtacc
    16381 tgcgccaatc aacgccttat cgccatacgc atcaacgatg gcgggaatgc ttttctccca
    16441 ttgaggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac
    16501 atgcgccagc gcctcgtcgg gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt
    16561 ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg
    16621 ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc
    16681 cggcgacaag ggtgatggcg tgttgatggg tcacatagtc gcgcatactg gcaacctctg
    16741 cgccaatcaa caaaccagag agaaattcgc tgacctgttc gcggggaagt gttcccagca
    16801 catgcgaggc gcgaacttca aaaagctgcg gcaatatggc gggagcatta aagccacgct
    16861 caagaccagc cgcgaaggca tcggcagagt tttcctgcgg cggcaaacct gcgccaatca
    16921 atgagtgatt taacagtaaa tgatgtaatt caccggtcat cacggtgcga aaatcgttga
    16981 tttgctggct atcggcctgc acccatttgc aatgggtacc gggcataaca taaagagagg
    17041 aaggagcgag agcacgcgcg ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt
    17101 tatcgtcatg agagacgcat aatccgggaa taatccagat attgtcgcca accgacgtta
    17161 attgatcgcc aatagacgaa aaacgggcag gaacagataa atacggtgca actttccagc
    17221 cgacattgct gccgaccatt cctgccatca ccactggcgt attctcttca cgccagtcgg
    17281 tcgtgacttc tgctaacacc gcagccggag attttccgtt caggcgcgtg acgcctgctt
    17341 ctgattgcct gctgtccagg cagtggtcgc cctgataaag ccaggcgcgc agattggtcg
    17401 atccccagtc aattgcgatg tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga
    17461 gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg ccgtcctgat gccgtattgc
    17521 atcgaacagc gccttatgtt cctggagcgt ttgcggcatg ttggcctcat cgcccatcca
    17581 ggttcgttca aaaaccgccc gctgcagcga actgatcgca atgctaagtt gctgtaacac
    17641 cgggttatgc accgactgca gcaccgcctc gtggtagcga atatccgctt cgttaaacgc
    17701 ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc gattcaatct gcgccagatc
    17761 gctggaggtc gcgcgctctg ctgcccaacg ggcaatagcc ggctctacca ggtttcgcac
    17821 ttcgctcatt gcactgataa gccgtgggtc atagtcgttt tccagcaccc attgcagtac
    17881 gtcagtgtcg aggtaattcc actggttacg cggagccaca aacgccccgc gataacgttt
    17941 catttcaatc agccgcttcg ccatcagcga acgaaacacc tcacggatga tgttgcgcga
    18001 ggttgcaaac tcttcacaga gctccgcctc agccggaagt ggagagcctg gtacgtattt
    18061 gccgtgaacg atctgtttac ccagcgtaat aacgatgcga tcggttttat tgagagtcat
    18121 ggagagtcct tgtgctctgt atgttcttct ctactttacc ccgatcgatg cataacgcgg
    18181 caactttgta gtaccagcgt gatgacgttc gcgtttgctg tgcgcctaat gtagtacaaa
    18241 cttatattgt tgtactacaa tttagatcac aaaaagaaca atacataaaa aatgacatgc
    18301 gtcgggcaga aatctgaaaa gggatgtcag gcgctaaaca ggagggaaag aagagtatgc
    18361 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    18421 tttaaggctg ccggtttgtg ttctttggca tttgcgttga ccggatgtat cacctgggga
    18481 ttggtttcca atactgcaag ccgtccacat gccgagcaat ggcggtcaga cacgattaag
    18541 ggtttatcgc tggctgaaga cagtaacggt acgaaaggct atgtttttgt tggtgaatcg
    18601 ttggactatc tgttgaccac cggcggcgat gaggtggtta aaatgctgaa cgatccggca
    18661 atccatggtg aaagaatcac cgtttctgat aacgcgaaat ttattttaag cagcagtaat
    18721 aagaatttct ctggtgctat tacgctctat tacgactgga acaacgaaga agataaggca
    18781 ctggcaacac aatatggttt tatttgtgat acccggcgtt gtacatggat gctggacggt
    18841 ttgaaaggct caatccacca gaaaaataaa aaagcggatt actcaaacgt gatggttttc
    18901 catcaaccct ttacggtggg attttatgag tacaaagcaa cagatggcgt cccgcgcggc
    18961 ctcgttaatg cgctgctgcc agtcactctt acgctggata tagtaacgtc accactccag
    19021 ttcctgattt tatgcacgac cagaaattgt taatcgctgc gggcaaacgc attcatgccg
    19081 tttgcccatt cactaattaa ttcagcacat acttctcaat agcaaatgcc acgccatctt
    19141 caaggttgga tttggtgaca aagttcgcca cttctttcac cgaaggaata gcgttatcca
    19201 tcgccacacc gacgcctgca tattcaatca ttgcgatgtc gttttcctga tcgccaatcg
    19261 ccatgatttc ttccggttta atacctaaca cgtcggccag tgatttcaca cccgtacctt
    19321 tgttaacgcg tttatcgagg atttcgagga agtacggcgc acttttcagc acggtgtatt
    19381 tttctttcac ttcctgcgga atacgcgcga tggcctggtc gaggatggca ggttcatcaa
    19441 tcatcatcac tttcaggaac tgggtattgg ggtccatttt ctccgcttcg cagaacacca
    19501 gcggaatggt ggcaacgaaa gattcatgca ccgtgtagta gctgatatca cgattggcgg
    19561 tgtacagcgt ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga gagagttttt
    19621 ccaggaaacg gtagtcgtca tagctgagag tggtttgcgc cacggtgcta ccatcagcgg
    19681 ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc gcccggctgt tccatatgca
    19741 gctctttcag gtaattgtgc acacctgcat aagggcgacc cgtcgttagc acgacattca
    19801 cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg gtgtgatcgg
    19861 gcagcagaag ggtgccatcc atatcgatag caatgagttt gatagccatg agttccccag
    19921 gtagattggt tcctgaccca tgctaacgcg attccgctca aaaatcagta caacacccga
    19981 gggaaaaggg ggatgcaacg cgcgttgcgt gctccccttt tgcttagcgg aagagtttcc
    20041 ctttcagcag ttccatgcct gcggaaagca gatcgttatt ggcttgtggt gacacttcac
    20101 cttgcggtga gagtgcatca ataatcttcg gcaattgttc tgccagtaaa ctggaagctg
    20161 tactggtatc cacgcccagt ttttgcccga gatcggacac cgcatttgtg ccgagcgccg
    20221 attccagttg ctcgccgcta actggttggt tgccctgttg attactcagc caggttgaga
    20281 gaatggcacc taaaccgcca ctttgcagtt tttccagcag cacctgaatg ccgccctgct
    20341 cctcaaccca acttaaaatc gcctgatatt tccccgcatc gcctttcaga aaggcgccga
    20401 caacgtcatc aaaaaggccc atgataatca cctgtaaagc gttacgtgtt gactcaaaaa
    20461 gtatagttct gtgggatgat aattgcggat tgcaggaata aaaagggcgg agatgatctc
    20521 cgcccttttc ttatagcttc ttgccggatg cggcgtgaac gccttatccg gcctacaaaa
    20581 acatgcaaat tcaatatatt gcaggagtct cgtaggcctg ataagcgtag cgcatcaggc
    20641 acgctcgcat tgttaacgcc attaaatatc aatattcgcc gctttcagag cgttctcttc
    20701 aataaacgca cggcgcggtt caacggcgtc gcccataagc gtagtgaaca actggtcggc
    20761 agcaatcgca tctttaacgg taacgcgcag catacgacgg ctttccgggt ccatggtggt
    20821 ttcccacagc tgttccgggt tcatctcgcc cagaccttta taacgctgga tggagaggcc
    20881 gcgacgggac tctttcacca gccagtccag cgcctgctcg aagctggcta ccggctgacg
    20941 acgttcgcca cgttcgataa atgcatcttc ttccagcaag ccacgcagtt tctcacccag
    21001 cgtgcagata cgacgatatt cgccgccagt gataaattcg tgatccagcg gatagtcagt
    21061 atccacaccg tgggtacgca cgcgaacaat cggctcgaac aggttttgtt cggcattggt
    21121 gtggacatca aacttccact ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag
    21181 cgcgttcacc cagcgggtaa cggtctgctc atcagagagg tcggcttccg tcagcgtcgg
    21241 ctgatagata agctctttca gcattgcttt cggataacga cgctccatgc ggttgatcat
    21301 tttctgcgtc gcgttgtact cagacaccag tttctctaac gcttcgccag ccaatgccgg
    21361 tgcactggcg ttggtgtgca gcgttgcgcc atccagcgcg atggagatct ggtactgatc
    21421 catcgcttcg tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg
    21481 cggctgagcg atgtagacgt gaccgcgttc aacgatttcc ggcatctgac gatagaagaa
    21541 ggtcaacagc agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat
    21601 gctgtgataa cgcagtttgt ccgggttgta ctcgtcacga ccgataccac aaccaagcgc
    21661 ggtgataagc gtcgccactt cctgggaaga gagcatctta tcgaagcgcg ctttctcgac
    21721 gttgaggatt ttacccttca gcggcagaat cgcctggttc ttgcggttac gcccctgctt
    21781 cgcagagccg cccgcggagt ccccttccac caggtacagt tcagagagcg ccggatcgcg
    21841 ttcctggcag tctgccagtt tgcccggcag gcccgctaag tcgagcgcac ctttacggcg
    21901 ggtcatttca cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga taattttgcc
    21961 aaccacgatt ttcgcgtcgg ttgggttttc cagcaggtat tctgccagca gttcgttcat
    22021 ctgctgctca accgccgatt tcacctcaga agaaaccagt ttgtctttgg tctgggagga
    22081 gaatttcggg tccggcactt tcacggaaac gaccgcaatc aggccttcac gcgcatcgtc
    22141 accggtggcg ctgactttgg cttttttgct gtagccttct ttgtccatgt aggcgttcag
    22201 ggtacgggtc atcgccgcac ggaagcctgc caggtgagta ccgccgtcac gctgcggaat
    22261 gttgttggta aagcagtaga tgttttcctg gaagccatcg ttccactgca acgccacttc
    22321 gacgccaata ccgtctttct cggtggagaa gtagaagata ttcgggtgga tcggcgtttt
    22381 gttcttgttc agatattcaa cgaacgcctt gatgccgcct tcatagtgga agtggtcttc
    22441 tttgccgtcg cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc
    22501 acgcagacgt ttcgccagaa tttcatattc gaactcggtc acattggtga aggtttcgag
    22561 gcttggccag aaacgcacca tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg
    22621 ggcctgcggt acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctggataac
    22681 cagctccagt ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc
    22741 ggacacttta taggagttat cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac
    22801 ttccgccgcc gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc
    22861 ctgtacagag acagagttat cggcgtgaat ggtgacgata atttctttac agtgacccgc
    22921 gagcgcttcg tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc
    22981 gtcatccgtg tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag
    23041 gactttgata ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact
    23101 tgggttaatc cgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca
    23161 tgtctataac gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta
    23221 agcggctggc aagcagcccg cgacgctcat catcaagctc agaggcaaaa tcatctatca
    23281 ggtagagaca ccgccgcccg ctttcacggg tgaggaactc tccttgcgcc agacgtaagg
    23341 cgcacatcaa cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg
    23401 cgcgaatgcg taagtccgct ttatgcgggc cgtgcgcggt gtaggttagc tggcgatcgc
    23461 gttcaaaatt acgttccagc acctcagcgt actctgtctc tttctcccag ccgcgctgga
    23521 aagagaaagt cagagaaaac tcagggagaa attgcttaca ggtatcggcc atatcagccg
    23581 cgataccggc gctatactcc gcgcgccagg tgcttatttg ctccgccagc ggaatcagtt
    23641 ctttatccca cgggcgtagc tgttcgtaac gtgtcacctg gcgcagcgcc gcattgcgct
    23701 gcttgagcaa tcgcttgaga ttgctccagg cggtgaaaaa tccgggttcg ttgtgaaagc
    23761 atccccagtc gaggaatgct cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt
    23821 ctggcgttat caactgcatt ggcatcaggt gcgccagttc cgcgacctta tgcccgtctg
    23881 taccgtcgat gcggactttg ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct
    23941 cgcgctcttc gccctgtaat cgcccgtgga gaacaaacgc ctcctgctcg tggcgaatga
    24001 cgcgaccaat ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag atggcttcca
    24061 gcacgctggt tttgccacta ccgttggcac ctaccagaaa gttaaagccg ggagataaag
    24121 cgagatccgc ggtttcgata ttgcggaaat cgcggatcaa caagcgggtg agggacatta
    24181 cagtctcatt ggcatgacaa cataggcagc cgactgtgat gcggcatctt caatctgcac
    24241 gctggaaacc gaatcggtca gcatcatgcg gacgttttcg catttcagtg cgttcagaac
    24301 atccagcaca tagctgacgt tgaagccgat ttccatctcc gcaccgctat aggtaacgtc
    24361 gaggatctct tccgcttctt cctgttctgg gttgttggcg gtgattttca gctggttttc
    24421 gctgacatac agacgcacgc cgcggaattt ctcgttagag agaattgccg cacgggcaaa
    24481 cgcctgcttg agcagatcac agccagcttc cagatgcttg tccgggttct tcggcagaac
    24541 gcgacggtaa tccgggaagc gaccatccac cagtttcgag gtgaagataa agtcgccaac
    24601 gtgggcgcga atgttgttgc tgccaatctg cacgcgcagc ggattgtcgc cgccgtcgag
    24661 catacgcatc agttcaatca cgcctttacg cggcacgatc accgaatggc ttggcaaaga
    24721 ttgaccaatt ggcattgaac agactgccag acggtggccg tcggtcgcca cggtgcgcag
    24781 ttcttcacct tcggtttcaa acagcatacc gtttaagtaa tagcgaacgt cctgatgcgc
    24841 catagaaaac tgggtcgctt caatcagacg cttcatcgtc gcctgcggca gggtaaattc
    24901 gacttcgctc tgccagtcat cgaggttcgg gaaatctgcc gctggcaggg tagacagcga
    24961 aaaacggcta cgcccggagc gcaccagcat ccgctcacct tccagctgca cggcaatttc
    25021 cgcgccttca ggcagaccac ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc
    25081 ccctggttcg tgcggctgaa ccagcgcaac gcgtgccacc atttccatct cgagatcagt
    25141 accggtcagc gacaacgtac cgtcagcaac ctgtaacagc agattaccga gaatcggtag
    25201 cgtaggacga ccacctaacg gaccgctcac ctgttgtagc ggttttaata aatgctcacg
    25261 ttctacggta aatttcatag gtttacgatg acaatgttct gattaaatta gaaaaatctt
    25321 ctttgatatc gtggctctct tcacgcaact gctcgatctt ccggcaggca tgaagcaccg
    25381 tcgtgtggtc acgaccacca aaggcatcgc caatctccgg aagactgtgg ttagtcagct
    25441 ctttcgccag cgccatcgcc atttggcgcg gacgggccac cgagcgggat cgacgcttgg
    25501 aaaggagatc cgcgactttg atcttgtagt actccgccac cgtcttctga atattgtcga
    25561 tggtgaccag tttttcctgc aatgccagca agtcgcgcag cgcctcacgc acgaagtcga
    25621 tggtgatcgc ccgtccggta aagttggcat tggcaatgac gcggttcagc gcgccttcca
    25681 actcacgtac gttagatcgt agacgcttgg cgataaaaaa cgccacttcg cccggcaaac
    25741 gaatgtcgtt ttcgtcggcc tttttcatca ggatcgccac gcgggtttcc agctctggcg
    25801 gttcgatcgc cacagtcagt ccccagccga agcgggattt caaacgatcc tcaacgccgt
    25861 tgatctcttt cggatagcga tcggaggtga gaatgatctg ttgattacct tccagcaggg
    25921 cgttgaaggt gtggaaaaac tcttcctgag atcgttcttt attagcaaaa aactgaatat
    25981 cgtcgatcag cagtgcatct acggaacggt agtagcgttt aaactcttcg atcgcgttgt
    26041 tttgcagggc tttaaccatg tcctgaacaa agcgctcgga gtgcatataa accactttgg
    26101 cattcggctt gcgcgccata atgccgttac ccaccgcatg cagcaggtga gttttaccca
    26161 aacccgtgcc gccataaagg aacaacgggt tataagcacc gccagggtta tctgccacct
    26221 ggcgagccgc cgcgcgcgcc agttggttag atttaccttc aacgaagtta tcaaacgtgt
    26281 gtttgacgtt tacgttagaa cgataggtcg gttctgccgg agccgggacg ttatcccaac
    26341 ccgagcgcgt agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg
    26401 cgacgttgct cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa
    26461 aacgcagctg tggggcatcc gctccgcaga aactggttag cagtccattg atattattaa
    26521 ggtacttgtc ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt
    26581 tatcgctcag ttccgcctgc aatgggcgta tccacatact gaattctgtg gctggtaact
    26641 catcctgcaa tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg gactccactc
    26701 gaacaaaagt cgataatgac taaggctgaa acattcatga ttgttgacgt acgtcgaaaa
    26761 gaccctgcat gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc
    26821 tgcaaaacga tcgggaccgc ggatcatagc ctaaactgcg caagagatct tctgtttctc
    26881 acagattttc ccgatttatc cacaggactt tccagaactc gctaagtgta aacgatcctg
    26941 ccgcgggccg ggcacgattt acgccgcata ttggaaaatt taatgaccat agacaaaaat
    27001 tggcttaatc gatctaataa agatccagga cgatccttgc gctttaccca tcagcccgta
    27061 taatcctcca cccggcgcgc catgctggtt ttcactggtg tgaggccgta cattttccct
    27121 gcgaaaaggt gcggaaaagc gcggtaaata aggaaagaga attgactccg gagtgtacaa
    27181 ttattacaat ccggcctctt taatcaccca tggcttcggt gtccatcgtt tcatttttcg
    27241 gcggatatcc aataaagcca ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg
    27301 cacttttcaa ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc
    27361 tactaaaaat ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac
    27421 cgtttctaag taataaagct aacccctgag tggttaagct cgcatttccc agggagttac
    27481 gcttgttaac tcccagtcaa ttcacattcg tcttccagca gccacaacgg gctggcacgc
    27541 cgcaaattac cattctcggc cgcctgaatt cgctggggca tccccgtatc ggtcttacag
    27601 tcgccaagaa aaacgttcga cgcgcccatg aacgcaatcg gattaaacgt ctgacgcgtg
    27661 aaagcttccg tctgcgccaa catgaactcc cggctatgga tttcgtggtg gtggcgaaaa
    27721 aaggggttgc cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc
    27781 gccactgtcg cctggctcgc gggtcctgat agccctcatt cgggtctatc aacgcctgat
    27841 tagtccgcta ctcgggccgc attgtcgttt cactccaacc tgttcaagct acggaattga
    27901 ggcattgcgc aggtttggag tgataaaagg cagttggttg acggtgaaac gcgtattaaa
    27961 atgccaccct ttacaccctg gtggtgacga tcccgtcccg cccggaccat ttgataccag
    28021 agaacactaa cgatggattc gcaacgcaat cttttagtca tcgctttgct gttcgtgtct
    28081 ttcatgatct ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc ccaacagacc
    28141 acgcagacaa cgaccaccgc agcgggtagc gccgccgacc agggcgtacc ggccagtggc
    28201 caggggaaac tgatctcggt taagaccgac gtgcttgatc tgaccatcaa cacccgtggt
    28261 ggtgatgttg agcaagctct gctgcctgct tacccgaaag agctgaactc tacccagccg
    28321 ttccagctgc tggaaacttc accgcagttt atttatcagg cacagagcgg tctgaccggt
    28381 cgtgatggcc cggataaccc ggctaacggc ccgcgtccgc tgtataacgt tgaaaaggac
    28441 gcttatgtgc tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg
    28501 gcaggcaaca cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc
    28561 aactacaacg tgcagaacgc tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg
    28621 aagcaatcca tcactctgcc accgcatctc gataccggaa gcagcaactt cgcactgcac
    28681 accttccgtg gcgcggcgta ctccacgcct gacgagaagt acgagaaata caagttcgat
    28741 accattgccg ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg
    28801 caacagtatt tcgcgacggc atggatcccg cataacgacg gtaccaacaa cttctatacc
    28861 gctaatctgg gtaacggcat cgccgctatc ggctataaat ctcagccggt actggttcag
    28921 cctggtcaga ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa
    28981 atggcagctg ttgctccgca cctggatctg accgttgatt acggttggtt gtggttcatt
    29041 tctcagccgc tgttcaaact gctgaaatgg atccatagct ttgtgggtaa ctggggcttc
    29101 tccattatca tcatcacctt tatcgttcgt ggcatcatgt acccgctgac caaagcgcag
    29161 tacacctcca tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt
    29221 ctgggcgatg acaaacagcg tatcagccag gaaatgatgg cgctgtacaa agctgagaag
    29281 gttaacccgc tgggcggctg cttcccgctg ctgatccaga tgccaatctt cctggcgttg
    29341 tactacatgc tgatgggttc cgttgaactg cgtcaggcac cgtttgcact gtggatccac
    29401 gacctgtcgg cacaggaccc gtactacatc ctgccgatcc tgatgggcgt aacgatgttc
    29461 ttcattcaga agatgtcgcc gaccactgtg accgacccga tgcagcagaa gatcatgacc
    29521 tttatgccgg tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct ggtgctgtac
    29581 tatatcgtca gcaacctggt aaccattatc cagcagcagc tgatttaccg tggtctggaa
    29641 aaacgtggcc tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa
    29701 taagggcggt cagttgaccg ccttttttct tttcgtaggg cggataagca ccgcacatcc
    29761 gccacacaaa gcaacaggaa catcatgagc gataatgaca ctatcgtagc ccaggccacg
    29821 cctccgggac gtggcggcgt tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt
    29881 gccgaaaccg tgctgggtaa actgcctaag ccgcgctacg ccgattatct tccgtttaaa
    29941 gacgccgacg gcagcgtgct cgatcagggg attgcgctat ggttccctgg cccgaactcg
    30001 ttcaccggcg aagatgtgct ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg
    30061 ctgttaaaac gcattctgac cattcccggc ctgcggattg ctcgcccggg tgagttttcc
    30121 gaacgcgcgt ttcttaacga taaactcgac ttagcccagg ccgaggcgat tgcggatctt
    30181 atcgacgcca gttcggaaca ggcggcccgt tcggcactta actcgctgca aggcgcattc
    30241 tccgcacggg ttaatcatct ggtagaagcc ctcacccact tgcgcattta cgttgaagcg
    30301 gcaattgatt tccccgatga agagatcgat ttcctctccg acggcaaaat tgaagcccag
    30361 cttaatgacg tgattgccga tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg
    30421 ttgcgcgaag ggatgaaagt ggtgattgcc ggacgtccta acgccggtaa atcgagcctg
    30481 ttaaacgcgc tggctggccg tgaagcagca atcgtaaccg atatcgccgg aactacgcgt
    30541 gacgtgttgc gtgagcatat ccacattgac ggaatgccgc tgcatatcat cgacaccgcc
    30601 gggctacgtg aagccagcga tgaagtggaa cgtattggta tcgagcgcgc gtggcaagaa
    30661 attgaacaag ccgaccgcgt gctgtttatg gtcgatggca ccacgacaga cgccgttgat
    30721 ccagcagaga tctggccgga gtttatcgcc cgtctgccag cgaaactgcc gatcaccgtg
    30781 gtgcgcaata aagccgatat caccggagaa acgttgggga tgagcgaagt gaacggtcac
    30841 gcgttaattc gtctctctgc aaggactggt gaaggtgtgg acgtgctgcg taaccatctc
    30901 aaacagagca tgggctttga caccaacatg gaaggcggct tcctggcgcg tcgtcgtcac
    30961 ctacaggcac tggaacaggc ggcagagcac ctgcaacagg gtaaagcgca actgttagga
    31021 gcctgggcag gcgagctgct ggcggaagaa ctgcgtctgg cgcagcagaa cttaagcgag
    31081 atcaccgggg aatttacttc tgacgacctg ctggggcgaa ttttctccag cttctgtatt
    31141 ggtaagtaac cgcgcttgcg aagccgcatt ctgactgtca gatgcggctt cgcttcattg
    31201 ttagcactcc tgttactcct caaccctttt tttaaacatt aaaattctta cgtaatttat
    31261 aatctttaaa aaaagcattt aatattgctt cccgaacgat tgtgattcga ttcacattta
    31321 aacaatttca gaatagacaa atttcttgag tattatactg tagcctcgtg tctttcgagg
    31381 ataagtgcat tatgaatatc ttacatatat gtgtgacctc aaaatggttt aatattgaca
    31441 acaagattgt cgatcaccgc ccttgatttg cccttctgta gccatcacca gagccaaacc
    31501 gattagattc aatgtgatct atttgtttgc tatatcttaa ttttgccttt tacaaaggcc
    31561 atctttcgtt tatttacttg ttttagtaaa tgatggtgct tgcatatata tctggcgaat
    31621 taatggatat tgcaaatgta atattcacag ggatcactgt aattaaaata aatgaaggat
    31681 tatgtaatgg aaaactttaa acatctccct gaaccgttcc gcattcgtgt tattgagcca
    31741 gtaaaacgta ccactcgcgc ttatcgtgaa gaggcaatta ttaaatccgg tatgaacccg
    31801 ttcctgctgg atagcgaaga tgtgtttatc gatttactga ccgacagcgg caccggggcg
    31861 gtgacgcaga gtatgcaggc cgcgatgatg cgcggcgacg aagcctacag cggcagccgc
    31921 agctactatg cgttagccga gtcagtaaaa aatatctttg gttatcaata tactattccg
    31981 actcaccagg gccgtggtgc agaacaaatc tatattccgg tactgattaa aaaacgcgag
    32041 caggaaaaag gcctggatcg cagcaaaatg gtggcgttct ctaactattt ctttgatacc
    32101 acgcagggcc atagccagat taacggctgt accgtgcgta acgtctatat caaagaagcc
    32161 ttcgatactg gcgtgcgtta cgactttaaa ggtaactttg accttgaggg attagaacgc
    32221 ggcattgaag aagttggccc gaataacgtg ccatatatcg ttgcgaccat caccagtaac
    32281 tctgcaggtg gtcagccggt ttcactggca aacttaaaag cgatgtacag catcgcgaag
    32341 aaatacgata ttccggtggt catggactcc gcacgctttg ctgaaaatgc ctatttcatc
    32401 aagcagcgtg aagcagaata caaggactgg accatcgagc agatcacccg cgaaacctac
    32461 aaatatgccg atatgctggc gatgtccgcc aagaaagatg cgatggtgcc gatgggcggc
    32521 ctgctgtgca tgaaagacga cagcttcttt gatgtgtaca ccgaatgcag aaccctttgc
    32581 gtggtgcagg aaggcttccc gacatatggc ggcctggaag gcggcgctat ggagcgtctg
    32641 gcggtaggtc tgtatgacgg catgaatctg gactggctgg cttatcgtat cgcgcaggtg
    32701 cagtatctgg tcgatggtct ggaagagatt ggcgttgtct gtcagcaggc gggcggtcac
    32761 gcggcattcg ttgatgcagg taaactgttg ccgcatatcc cggcagatca gttcccggca
    32821 caggcgctgg cctgcgagct gtataaagtc gccggtatcc gtgcggtaga aattggctct
    32881 ttcctgttag gccgcgatcc gaaaaccggt aaacaactgc catgcccggc tgaactgctg
    32941 cgtttaacca ttccgcgcgc aacatatact caaacacata tggacttcat tattgaagcc
    33001 tttaaacatg tgaaagagaa cgcggcgaat attaaagggt taacctttac ctacgaaccg
    33061 aaagtattgc gtcacttcac cgcaaaactg aaagaagttt aattaaaaca ctcagagtgg
    33121 ctataaggat gttagccact ctattaccct acatcctcaa taacaaaaat agccttcctc
    33181 taaaggtggc atcatgactg atcaatcaga aaaaaaacac tctgcatttt ggggtgttat
    33241 ggttatagcc ggtacagtaa ttggcggtgg gatgtttgct ttacctgttg accttgccgg
    33301 agcctggttt ttttggggcg cttttattct tattatcgcc tggttttcaa tgttacattc
    33361 agggctatta ttattagaag caaacttaaa ttatcccgtc ggatccagtt ttaacaccat
    33421 caccaaagat ttaatcggta acacctggaa cattatcagc ggtattaccg ttgccttcgt
    33481 tctctacatc ctcacttatg cctatatctc tgctaatggt gcgatcatta gtgaaacgat
    33541 atcaatgaat ttgggttatc atgctaatcc acgtattgtc gggatctgca ctgccatttt
    33601 cgttgccagc gtattgtgga taagttcgtt agccgccagt cgtattacct cattgttcct
    33661 tgggctgaag attatctcct ttgtaattgt ttttggttct ttcttcttcc aggtcgatta
    33721 ctccattctg cgcgatgcca ccagcaccac ggcgggaaca tcttatttcc cgtatatctt
    33781 tatggctttg ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc ccagcctgat
    33841 tatttgctat ggcaaacgca aagataagct aatcaaaagc gtggtcttcg gttcgctgct
    33901 ggcgctggtg atttatctct tctggctcta ttgcacgatg gggaatatcc cgcgagaaag
    33961 ctttaaggcg ataatctcct caggcggcaa cgttgattcg ctggtgaaat cgttcctcgg
    34021 caccaaacag catggcatta tcgagttttg cctgctggtg ttctccaact tagctgttgc
    34081 cagttcgttc tttggtgtca cgctgggatt gttcgattat ctggcggatc tgtttaagat
    34141 tgataactcc cacgccgggc gtttcaaaac cgtgctgtta accttcctgc cacctgcgtt
    34201 gttgtatctg atcttcccga acggcttcat ttacgggatc ggcggtgccg gactgtgtgc
    34261 caccatctgg gcggtcatta tccccgcggt gctggcaatc aaagctcgca agaagtttcc
    34321 caatcagatg ttcacggtct ggggcggcaa tcttattccg gcgattgtca ttctctttgg
    34381 tataaccgta attttatgct ggttcggtaa cgtctttaac gtgttaccta aatttggcta
    34441 aatccttcaa gaagccagcc attcgctggc ttcttgcctc tcaggaaatc acttatgtcc
    34501 aaatggcaac tcgcctgatc ctccttcacc acgtatgctt tgcgtcacct tactatcagg
    34561 acgctttagc ccatgtcccg ctttttgatt tgtagttttg ccctggtttt actttatccc
    34621 gcagggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct caatgccagc
    34681 gaagcgcagt tgcatattgc gttctccgtt tatctggcgg ggatggcggc tgcgatgtta
    34741 tttgccggta aagtggccga tcgttcaggg agaaagcctg tcgccatacc cggcgcggcg
    34801 ctatttatta tcgcctcggt gttctgttca ctggctgaaa ccagcgcgtt atttcttgca
    34861 gggcgatttc tacagggact gggcgcgggc tgttgttacg tagtggcgtt cgccattttg
    34921 cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cgttactcaa cggcattacc
    34981 tgcatcattc cggtgttagc gccggtgctc ggacacctga ttatgcttaa attcccttgg
    35041 cagagtctgt tctggacgat ggcaacgatg ggcatcgcgg tactgatgtt gtctttgttt
    35101 attttaaaag aaacgcgccc tgcggccccc acaacttcgg acaaaccacg agaaaatagc
    35161 gagtcactgc ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct cagcgtttcg
    35221 gtgatcctca ccttcgtcaa cacgtcgcca gtattgctga tggaaatcat gggttttgag
    35281 cgcggagaat acgccaccat tatggcgttg accgctggcg tcagcatgac cgtttcattc
    35341 tccacgccgt ttgcgctggg aatttttaag ccacgtacgt tgatgatcac ctcacaggtg
    35401 ttattcctgg cggcggggat cacccttgcc gtttcacctt cccatgcggt ttcgctgttt
    35461 ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg gtgtggcgat gagtcaggcg
    35521 ttagggccgt tttcattacg cgcgggcgta gccagctcga ccttaggtat tgcgcaagtt
    35581 tgcggttcgt cactgtggat ttggctggcg gcggtggttg gtatcggcgc atggaatatg
    35641 ctgatcggga ttctgattgc ctgtagcata gtgagcctgt tgctgattat gttcgtcgcg
    35701 cctggacgcc cggttgccgc tcatgaagaa atccatcacc acgcttgatc tcaatctgct
    35761 gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga agcggatgaa
    35821 cgtgacccct tcggcggtga gtaagtcgct ggcaaagtta agagcgtggt ttgacgaccc
    35881 gctctttgtg aactcaccgc tggggctgtc gcccacaccg ctaatggtca gcatggagca
    35941 aaatctggcg gagtggatgc aaatgagcaa cctgctgctg gataaaccgc accaccagac
    36001 accgcgcggc ctgaagtttg agctggcagc agaatcaccg ctaatgatga tcatgcttaa
    36061 tgcgctgtcg aaacggatct accaacgtta cccgcaggcg accatcaaat tacgtaactg
    36121 ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt tttccggtcg
    36181 cgaaagtcat ccccgttcgc gggagctgtt aagctcgcta ccgttagcca ttgattatga
    36241 agtgctgttt agtgatgtgc cctgcgtctg gttacgcaaa gatcatccgg cactgcatga
    36301 agcatggaat ctggacacct ttttacgtta tccgcatatc agcatttgct gggaacagag
    36361 cgatacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat
    36421 gagcctgccg gaattcgagc agtcactgtt tatggcagca cagcccgaca atctgctact
    36481 ggcgaccgca ccgcgctact gtcagtacta caatcaactc caccaactgc cgttggttgc
    36541 tcttcctctc ccgtttgacg aaagccagca aaaaaagctg gaagtccctt ttaccctgct
    36601 gtggcataaa cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa
    36661 tctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa
    36721 tgtaaaaaag ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat
    36781 cattccagta aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat
    36841 ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc
    36901 ctcccagtgt catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc
    36961 gtccagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga
    37021 aatcgtcacc ctgccgaaag gaaaaccggt tttcagcgat aaaaatttgc cttcgttttc
    37081 tatttcctat gccgggaata tggtcggcgt ggcgttaaca accgaaggtg aatgtggcct
    37141 cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca
    37201 caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg
    37261 ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga
    37321 cgatccgcgc gatctgcaac tactgcccat tgccggacgc ctgaaatgtg ctcatgtaaa
    37381 tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc
    37441 cacgattgaa aagctcagtg tctgggagtt agatggcaaa catggctgga aaagcctgcc
    37501 cgatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc
    37561 taccgtgaag gctttttcgc caaattgata gacaaccaca ggagtcatca tgtctgaaaa
    37621 attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc
    37681 acgtaccctg ccgaaaattg ctccggcgag catggaaatc aatgcgttac cttccattgc
    37741 cgacattccc ctgtatgacg ctgacgtaca gcaggaagac ggttttccag caacagttga
    37801 agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa
    37861 ctactcggta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca
    37921 accgctggct ggtaaaccgg tattgattca gaccagctca atgggcgtga ttggcggcgc
    37981 gcgctgtcag tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa
    38041 gccggaattt atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gggaagtgat
    38101 tgatcagggt acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca
    38161 gcgagttaag atctaaataa aaaacccgcc agcaatcatg catggcgggt ttttaacgcg
    38221 ctatcgattt tagtgagcgt cgataaagac aatcttcagg ataaacagca gcgcaacgat
    38281 gattacgcac gggctaaggt cacgcagacg tccggtacca atcttcatca cgcagtagga
    38341 gataaagccc agcgcgatac cttcagtaat cgagaagctg aacggcatca tcacagcggt
    38401 aataaacgcc ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt
    38461 catcaacacg ccaacgtaaa tcagcgcacc agctgcagcg tagcctggca ccatccccgc
    38521 cagcggcgac aggaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc
    38581 ggtacgaccg ccaacggaaa cgccggaaga agattcaata tacgccgtaa cagaagaagt
    38641 accgataaac gaaccggtca ccgaagagat actgtcgaca tacagcgcct gcttcatgcg
    38701 cgggaattta cctttctcat cagccagacc tgctttatcg gtcacgccaa tcagcgtacc
    38761 ggaggagtca aacaagttga ccagcatgaa agagaaaatc acccctgcca gcccaaggtt
    38821 aaacgacccg gctaaatcta catgacccac gacactcatt acgctcggcg gcgcagaaac
    38881 gatgccatta tagtggacat cacccagcat ccagcccagc agcgttgtca ccacgatgga
    38941 aaccagcacc gccgcgtgaa tattgcgcga ggccagaatg gcgatgatga agaagccgag
    39001 gatccccagc agtacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgggtt
    39061 agcgacaatc acacctgcat tcttcagtcc catcatgcca ataaacaggc cgataccgct
    39121 ggtaatgccc acacgcagac tcaccggaat gttggctatc atccagtagc gaacgcggaa
    39181 aatcgtcagt aacagcagac caatcgcgcc ccagaagatt gcgcccatcc cgacctgcca
    39241 cggcaagccc atcgcctgca caaccacaaa agcgaagaac gcattcaggc ccatagcggg
    39301 tgccagtgca actggcaggt tagcaaacag tcccatcata atactgccga atgcagcaat
    39361 cagacaggta gtaacgaaga cggcgctggt atccatgcca gcaacgccaa gaatttgcgg
    39421 gttaacaaaa acgatgtaaa ccatcgtcag gaaggtggta aaaccggcga tcacttcggt
    39481 ccgtgccgtc gtgccatgtt cgcgcagttt aaacacgcgt tccagcatcc cctggccaga
    39541 agcctgggtt gtgtgttgtt gactcattat ctatttccga acaaggaggg aaaatccgtc
    39601 gctatcgtat accaaaatga gacaatatgg acggctgaag ataacttttt ttcattgtat
    39661 ttgcttatac ggcaacgatt gcgtgatgcg aattggcatg acgaaaacgt taaactaacg
    39721 ataaaggaaa gacatgtccc agatagaagc ggtatttttc gactgcgacg gtacgctggt
    39781 cgatagcgag gtcatttgct ctcgcgccta tgtcacgatg ttcagggaat ttggcattca
    39841 cgttgacctg gaagagattt ttacccgttt taaaggcgta aaactctacg aaatcatcga
    39901 catcatcagt aaagaacagg gcgtgacgat ggtccaggcc gatgccgagc acgtttaccg
    39961 cgcagaagta gcacgtctgt ttgataccga acttgaggac atcgcaggag cgaacgaatt
    40021 gctggcgagc attaacgtgc cgatgtgcgt cgtctctaac ggtccggtca gcaaaatgca
    40081 gcattcgctt ggtaagctca acatgctgca ctacttcccg gaaaaactgt tcagcggcta
    40141 cgatatccag cgttggaagc ccgacccggc gttaatgttc catgcggcaa aagcgatgaa
    40201 tgtgaacgtt gaaaactgca ttctggtcga tgactccagc gccggtgcgc aatcaggaat
    40261 cgatgctggt atggaagtgt tttacttctg cgctgacccg cacaataagc cgatcgttca
    40321 tccgaaagtc accaccttta cccatctttc gcaattaccg gaactgtgga aggcgcgtgg
    40381 ttgggatatt acggcatagt tattcacact cccttcactt actccactta aattggcacg
    40441 caaaggtaag taaagggagt ttgatatgtc tgtttcacgt cgggtaattc atcacggact
    40501 gtattttgcg gttttagggc cgttaattgg tgttctgttt cttgtcctct acatattctt
    40561 cgcaaaagaa ccgctgattc ttttggtaat aatacaggta cttccattgt ttctcttaat
    40621 gtcgataact acgggagcta ttcctgcaat gttaactggc gtaatggtcg cctgtctgcc
    40681 ggaaaagatc gggtcacaga aacgttatcg ttgtctggtt ggcggcatag gtggagtcgt
    40741 tatcaccgag atctattgcg ctgttatcgt acatattaag gacatggctt cctcagcgtt
    40801 gtttgaaaac attctttctg gtgaaaatct cgttgtccgc atcatcccag cattgctggc
    40861 aggcgtagtg atgagcagaa tcattacaca tctacccgga ttggatattt catgtcctga
    40921 aacagactct ttaagctaag cggaatgctt tatctatttg tcattaaatg ttggagaaca
    40981 ggccggagcg taattcacac atccggcctt atttttaagc ttaaccgagc gctaattctg
    41041 cggcagcggc tttatcagcg atgactatca gcgaagggtg caactgcaat acggaagcag
    41101 gaacatcttc ggtaaccgga ccttgcagaa catttttcag tgcctgcgct ttacccgcgc
    41161 cactgacaat tatcagcaag tttttcgccg ccatgatgct tttcggcccc atcgtcacgt
    41221 agctgtcagg cactaatgaa aagtcgccac ccaactcgcc atgcgccaca atatcaacca
    41281 tctctccctg gatcgggaac tccacggttt gctcatggaa atgggtcgta tttggcagat
    41341 tgccacaaaa atgaccatct acacctaatc ccaacaccac caaatccagg ccgccttcac
    41401 gcgccagttt ctgatcatgc tcgcggtagt tatcaatagt gagcttctgg atattctctt
    41461 ctttgatccc tgcaggggtg aaaaacagat tatgcagatt ggtaatcgtt acgccttccc
    41521 cttctttacc gcgaaatgga atttcatcga aattgtagaa atagcagtta tcgtaccagg
    41581 gcttaccttt aaccagagtg atgagatatt cgtacatgcc tttgggcgtg ctcccggcgg
    41641 taattgccag gttaacgcga cgcatctttg acatataacc aagcagatga tgtgccgcga
    41701 cacggctcat ttcctggtaa tcttcggtaa tgatcaattt catcttaatg ccttatatta
    41761 tttgaataat ttctgtacaa aggctgcata ggctggtcgc cagacatcca tttcatggtt
    41821 cagaccggga tattcctggt aatcaaactt aatttttttc tgctcaagct cagttttcag
    41881 cccggcgata tccttgccgg ttacgacatc tttatcgccc acgaccacag taaaattacg
    41941 tagttgctgg ttaatagctg ccggctcgtt cagccgggca gcgacccctt catctggtac
    42001 ggtcgtcgtg gtaacaccac tgaatgtggc cagccagcca aagctttcca gatgattcat
    42061 tcccgatacc agtgcctgat acccaccttg tgaaagccct gccagtgcgc gaccatcggc
    42121 atctttacgg acattaaaac gcttgctaat cagcgggata atatcgttca tcaattcgcg
    42181 atctgccgct ttagcattta acggataaaa gactttacgt ctctcctgag gaacgaaatc
    42241 ttcgggaata atgcccttcg catcggtttc ggtatccggg atcaccacca gcatcggttt
    42301 aattttccct tcagccagca ggttatccat gatttgcggg atacgcccct gatcaatagc
    42361 ggaacgtccg gtatcaccaa agccgtgata gaaatagagc actggcaaag gctcgcctat
    42421 gccggtgtat cctggcgggg tccagacata catctgacgt tcggattgca atgcgttgga
    42481 gtggtaagtt atggcgatca aatcaccatg cacaacagaa cgtgtatcca gataactgcc
    42541 cggtaccaga atcatactgg agttaacctg gcgctgaggc ttagtcattg ctgtacctgt
    42601 atcaatgctg cgtacaccat caacattgaa aaaatactcg tacaggttgc ctttcagagc
    42661 aggcgtgcgc cacgaccaga ctcccgcttc gtctttggtc atcgggtgaa tattgtccgg
    42721 aaccggaaca cccaccacta cagagacatt ttttgcccca ggggcaaagt agcgaaaggt
    42781 cacgctgtta tcggtattga cctgagtcac atactgtttc accggaatag tgggtgccgg
    42841 gctggctggc atatccgctg ctatggccca ttgagcagaa ataccgctgg caatagccag
    42901 cgttaaagca gcaattttta tattcatcgt aatattcctt ttattaagtt ccattaccac
    42961 cagatttcag cctgggcacc gacagcaaac tggtcgtctt tattgtcttc aaaggtgaag
    43021 ccatccagtt cgttttccag agctttgata tatgtagcgt agaaacgaat ttccgggcgc
    43081 gaggtcaaca tactggtatt gactttaaag gtatggaaga gcgtggtttt ataaccagac
    43141 tcattatatt tattgctatt cgcatctttg ttttgctggg tgaaatagcc cagttcaaca
    43201 cctgtctggt tatattggtc ccaaatatag gccgggcgaa caaccgcacg aatagattcg
    43261 aaatcagaat gtgcgcctgt ttcgtagcta taaatatcgt taccgaagga gtaaacaatc
    43321 gcgttagcca caatgaaatg atcgccgata taagcttcgc cctgcgaagt cagacgaact
    43381 gcagttccgc ctgtgtgatc accataataa cgaccattaa aggtagtaaa tggacttgca
    43441 ccagcataac gaccaaagtt actggcgata gagttattcg caaccaggaa ggagaattcg
    43501 ttgaagccac ccttatcaaa tttctgcgtt aaagatgtac caaacatcca ggtatctttc
    43561 cagtcataat acccgttatt atcctgatta tctttttccg atgcgctttc atttgctgag
    43621 acataacgac cgctcaccat taaggtggct ttatcccata acgggatatc tttatagcgt
    43681 aaatcaatgg tattggtatt aatctgctgt ttgttttgca ggctgcgatc gtaatcatcg
    43741 acatcttcac gaaccagcgc gatatcaatt ttacccggac cgactttcca gttttccaga
    43801 cctacacctg cggcagcatc ggtacgctgc gttttccagt caagcatctg gatttcaatt
    43861 ttcggcgcac cgtgtttacc cacccagaaa tcagcctctg gcgcaaaggg caggaaacct
    43921 ttggtggtaa cgtacatatc agagaactgc ataaagttct caccaccggc gttatcgcca
    43981 aaccagccag tagagtactg ctgaccaacg ttaccatcca tcatcacgat ggcatcaacc
    44041 cgtttgccgt tttcgttgta gacacgttgt tttaactgca aatcaaacca gccggagtat
    44101 tcgttaccaa agcggcccag agaaccaatt gcccatgatt taggtgaacc atgagaggct
    44161 gttccccaac cagaacggta gtagccgttg taactaaagc cgatttcatc tttcacaaat
    44221 ttactgaaat ctttcagagt catggaagaa taaatcgagc ctgtctcact ggcatttttc
    44281 tcttcatttt taaccagtac agcatcaggc tttgcggcac gactggtcgg gaatggattg
    44341 gcggcatacc cttgatcatt cgtacttacg ctacgattca ccgtcgctgg cgtatacttt
    44401 ttcttctctt catctttata ttttttcagt tcgctttgcg tttctcttaa cgccttttct
    44461 aatagctcaa ggcgttgttc cactgttaat gattcagcca atgattgtgc ggaaaaggct
    44521 aacggtgcca ttaataagat ggcagaggta ataagatttc gtctaaacat aatatccctt
    44581 tatggtgcaa agaaagaatt aacgcatcgc atccagactg ttctgaatgc gacgataatt
    44641 aaggtgcttt aatggttatt tttttaatga cagcccccga gttttgatca cttctgcata
    44701 ccatccgaag cttttcttgc gcgttcttgt gaggcttcct tcaccattat catcacgatc
    44761 cacataaata aagccgtagc gcttagacat ttgtgaatga gatgcactga ctaaatcaat
    44821 tggcccccaa ctggtgtacc ccataatatc cacaccatcg gcaatcgctt catttacctg
    44881 taccaggtga tcgtttaaat aggcaattcg ataatcgtcc tgtatcgaac catccacttc
    44941 aacgctgtct tttgcgccta atccgttctc gacaataaat aacggttttt gataacgatc
    45001 ccaaagcgta tttaacagaa tccgtaatcc aaccggatca atttgccacc cccactctga
    45061 acttttcaga tgcggatttg ggatcatatt cagtatgttg ccctgcgcat ttttattaat
    45121 gctttcgtcg tgggaaacac aaccggtcat gtaataacta aaagagatga aatctacggt
    45181 atgttttaaa tcttctgcgt cactttcagt catctcaatg gtgatattgt ggtcgcggaa
    45241 gaaacgctgc atatagccgg gatactgacc acgcgcctga acatcaccaa agaacatcca
    45301 gcgccggttc tcttccatgg cctgcaacat atcctgtggc tggcaggtga gggggtaaac
    45361 cagcccaccg agaagcatat tgccaatttt cgcttcgggg atcaggctat gacaggcttt
    45421 aactgcccgc gcactggcaa ccagttgatg atggatcgcc tgataaactt ccgcctcgcc
    45481 actctcttct gccagcccca ctcccgtgaa tggcgcgtgt aatgacatgt tgatttcatt
    45541 aaatgtcagc cataacacaa ctttatgttg gtagcgggtg aagaccgtgc gggcgtaatg
    45601 ctcgaagtga tcgatgaccg cccgattagc ccaaccgccg tagtttttca ccagcccata
    45661 tggcatttcg taatgggata acgttaccag cggcttgatc cccgcctgcg ccatttcatc
    45721 aaacagccga tcgtaaaacg ctaaccccgc ttcattcggt tcggcttcgt cgccctgagg
    45781 gaaaattcgc gcccaggcaa tggaaatacg cagacaggtg aagcccatct cggcaaataa
    45841 cgcgatatct tccgggtaac ggtgataaaa atcgatggcg acatctttga tattctcttt
    45901 ccccaggatg cgcggttcca tttttcccat tacgccatga ggctgtaaat ctgaggtcgt
    45961 gatccctttg ccatcttcct gccaggcgcc ttccacctga ttggcagctg ttgcaccacc
    46021 ccaaagaaat gtttctggaa atgctttcat aattaactcc ttttatcgtt agcgaatgat
    46081 ggataacagc ggctcaccag cgtttatctg cgccgtgccg tggggtaata cgtccgtaaa
    46141 atcatcgcta ttactgatta ataccggcgt cgtcagatca aatccggcct cgcgaatagc
    46201 agggatatca aaagaaatca gccgatcgcc tgtattgacc ttgtcaccca cgttgacgtg
    46261 agcggaaaag aatttgccgt ccagttttac ggtatcgata ccgacatgaa tcaggatctc
    46321 cacaccatca tctgactcaa tgccaatggc gtgtaatgtg gcgaacaacg aagcaattcg
    46381 acctgccacc ggagaacgca cttcaccaac cgagggcata atggcaatac ctttacccaa
    46441 caggccgctg gcaaacgtgg tatcagcgac gtgaatgagc ggcacaatct ctcccgtcat
    46501 cggtgaacag ataccgccct gctcaggtgt aataacctct ggtgttttct cttgctgggc
    46561 aacttgcgct gactgactct tagcggtgat gaaatgaaac atcaccgtac cgacaaatgc
    46621 gcaaccgatg gcaatgacac cgccaataac gctggcccat acggtgaaat caatccccgt
    46681 tgacgggatg gtttgcatga aggtgaaaat acctggcaaa ccaaaggagt aaactttcgt
    46741 ttgcgcgtag ccaatgatgg tggcccccaa tgccccactt atgcaggcga taacgaaggg
    46801 gtacttacgc ggcagattga cgccatatac cgctggttcg gtgataccaa acaaacctgt
    46861 caacgccgct gatcccgcca ccactttttt ctgtgcatcg cgttcgcaga ggaagacgcc
    46921 gagcgccgcc ccgacttgcg ccataatggc gggcattaac agcgggatca tggtgtcgta
    46981 gcccagcacg gtgaagttat tgataattaa aggtatcaaa ccccaatgca gaccgaacat
    47041 cacaaagatt tgccagaagc cgcccattac cgcgcctgca aatgcaggaa ccgcctgata
    47101 aagccagaga taaccggcag caatcagttc acttatccag gttgatagcg gccccaccag
    47161 cagaaatgtg atgggcgtga tgaccatcag acatagcaag ggtgtgaaga aatttttgat
    47221 tgccgaaggt aaccacgcat taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa
    47281 aataatggga ataaccgatg acgagtaatt caacaatgtg accggaatac ccaggaaatc
    47341 caggcccagc gcatccactt tttgcccgtt ctcgaaggca gtcagaatta atggatgcac
    47401 taacgctccg ccaatcacca tggcagtaaa tggattgccg ccgaagcgtt tccctgcggt
    47461 gtagcccagg attatcggga agaaccaaaa caaggcatca ctggcgctaa ataaaattaa
    47521 ataagtacca ctttgttcgg tcgtccactg aaaagtgagt gccagagcca acataccttt
    47581 caggatcccg gttgccgcca tcaaaccgat cagaggcgta aaaatacctg aaataacata
    47641 aacaaagcgg tttaacagat tacctttatc atcattttcc ggtgcctgtt gcgctttttc
    47701 gccaaggcct gccacgctgt taaccgccag gaagacatcg gccacatggt tacctatgac
    47761 aacctgaaac tggccaccgc tttccaccac cataataata ccgggggtct ttttcagtac
    47821 ctcagcttgc gctttgcttt catcctttaa cttaaaacgt aatcgcgttg cacaatgcat
    47881 cagactcaca atgttatctg cgcccccgac tcctgcgact attgttctgg ctaactccgt
    47941 cataacttgc cctctaccgc tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc
    48001 ctgacgtgaa tcaagcaatt tttttcaggt tttgcccgct tagtgcggta acaatccttt
    48061 attcagtaat aatatttcag tgttctttgc gcacgcgctc tatatttatg gctaaaaaca
    48121 taatctctgc aggtgagatt ttacgttgat actgcaaacc aataaaaatg gcgatccgct
    48181 ccgcacattg ccatgcttgc gggtaatttt gctttactgc ttgttgtaat gattcatcac
    48241 tatcgttaat cgatgcatgt tcaataatac gccaggataa aaatttcaga tgcgtaacca
    48301 gtcgctgata actcaagctt tcttcctggt aattaaggct gaactgaaat tttattaatt
    48361 gcagcatttc tcgcattaat tgcgtgacac ctgcaacatc ctccatattt ccgctcattt
    48421 gggcactgac cagatgcatg gcaataaagc ccacttcatc tttcggtaac tgcacgccca
    48481 accgtttaac aataatggtt aacgcttctt cccctagctg gaactctttc gggtaaagtc
    48541 gctggatatc ccacagcagc gggttgggca gtagcacgtt ttgctgaaag cgtttaatcg
    48601 caaactggca atggtcagtt agcgagatat aaatactgtc ctgcaacttt cccaggcgct
    48661 cctgcgccag agagataata cgatcacagg ttgccatcac ctcaagaggc atatgactta
    48721 agagttcgct taatcgcccg ttcagttcat gactgctcaa ggcatactct ttttctattc
    48781 cacttgagtt aattctttcg cctgggcgtt tttgaaagcc aattccgcgc cccatgacga
    48841 ctttttcccg ctgttgatca tcaataacca ccacaacatt attgttgaga attttggtga
    48901 tttgcatgtt catagcaagg acctttttta taaacaaaaa aacccgactt caccagtatt
    48961 ctctggttat gtcaggtttt gcctgcgaat gcagtaacaa tccagtcatt aattaatggt
    49021 ttttataacg aacatccagg ttcgaaaatt aatttagttg cgtgcagaga aaatagcaat
    49081 gctctattta taaaagtatg atcatgctcg cagttattaa ttttgtgtaa ttttaggatt
    49141 ttataaagtt atatataaca aatcccaata attaaattat tgggatttac ttggtgaatt
    49201 atttatcgct atctttttct gccagcagtt tatcgagctc gtcaccaccg acgtgacgga
    49261 aatcctgccc cttcacgtag tagaagataa actcacaaat attctggcag cggtcgccga
    49321 tacgttcgat tgaacgcgca caaaacagcg cggtaagtac gcttggaatg gtgcgcgaat
    49381 cttccatcat gtaggtcatc agctgacgaa caataccttc gtactcctga tcgacttttt
    49441 tatcttcacg ataaatacgt accgcttcat caatgtccat ccgcgcgaac gcgtccagca
    49501 cgtcgtgcag catctggatg gtatgacggc ccagcgactc cagacttacc agcaatggct
    49561 gatgctgctg ggagaatttc tccagtgcag tacggcagat tttgtccgcc acgtcgccaa
    49621 tacgctccag ctcggcaatg gttttactga tcaccataac cagacgcagg tcgctcgccg
    49681 tcggctggcg tttggcaata atgcgcacgc aggcttcatc gatcgccact tccatcatgt
    49741 tgacgttctt gtcgccttcg atgacgcgct tcgccagatc gctgtcctgg ttgtgcattg
    49801 cagtgatcgc atcagaaagc tgctgctcca ccatgccgcc catggtcatc acctgcgtgc
    49861 ggatgctttc cagttcggcg ttgaactggc cggaaatatg tttattaaga ttgagactgt
    49921 ccataacgca ctcctgaatc aaccgtaacg accggtgatg tagtcttctg tttgtttctt
    49981 cgctggcttg gtgaacagat cgtccgtgtt gctgaactca atcaattcgc ccaggtacat
    50041 aaacgccgtg tggtcggaac aacgcgcagc ctgctgcatg ttgtgggtga cgatcaccac
    50101 ggtgtaatcc tgcttcagtt cggtaatcag ctcttcaata cgcccggtgg agatggggtc
    50161 gagcgccgaa catggttcgt cgagcagcag cacttccggg cgaatggcga taccacgcgc
    50221 aatacacaaa cgctgttgct gaccaccaga gagagagtaa ccgctctggt gcaatttatc
    50281 tttggtttcg ttccacaatg cggctttggt caatgcccac tgcacgcgct cgtccatgtc
    50341 agcacgggag agcttctcaa acagacgcac gccaaaagcg atgttgtcgt agatggacat
    50401 cgggaacggc gtcggtttct ggaacaccat gcccactttc gcacgcagca gggcgatatc
    50461 ctgagagttg gtcaggatgt tgtcgccatc gagcagaatt tcaccttccg cacgctgttc
    50521 cgggtacagt tcaaacattt tgttgaaggt acgcagcagc gttgatttac cgcagccgga
    50581 cggcccgata aacgccgtaa cctggttttt ggcgatatcc aggttgatgt ttttcagggc
    50641 atggaatttg ccgtagtaga agttcaaatt acgaacctga attttactcg gggcagtttc
    50701 aaccatactc attgcaatct tttcctcatc gtgcctgatg cacttcgcga atcaggctta
    50761 ccgtaggccg gataaggcgt ttacgccgca tccagcaaag tgttaaccgt gtttattctt
    50821 cgcaaaaaca acgcgcgcca gaatgttcag cagcagtacg cacagggtaa tgatcaacac
    50881 cccggcccag gccagttgct gccattccgc aaacgggctc atcgcaaact taaagatcgt
    50941 caccggcagg ttggcgatcg gctgcatcat gtccgtgctc cagaactggt tggagagcgc
    51001 agtaaacagc agcggcgcgg tttcaccagc aatacgggca atcgccagca ggataccggt
    51061 catgatcccg gacaccgacg ctttcagcgt gatcgcggca atcatcttcc acttcggcgt
    51121 acccagcgca taagccgctt cacgcaggct gtccggcacc agtttcagca tgttctcggt
    51181 ggtgcggata acaatcggca cctgcaacag cgccagcgca atcacgcccg cccagccgga
    51241 gaagtgctcc atctgcgcca ccacgatggt gtaaacaaaa agaccaacca caatcgacgg
    51301 tgcggagagc aaaatgtcgt taatgaagcg aatcacttct gccagccagg atttacgacc
    51361 atattcagcc agataaatcc ccgccataat gcccagcggc gtaccgaata ccgtggccca
    51421 caaaattaac aggccgctac ccgccagggc gttcgccaga ccaccgcctt ccgtatttgg
    51481 cggcggcgtc atttcggtga acagcgccag cgacatacca tcgataccgc gagtgatggt
    51541 ggacattaaa atccagatca gccagaacag accgaaggcc atcgtcgcca tcgagagcgt
    51601 cagcgcaata cggttcttaa ggcggcgacg cgcctgcatt ttacggcggg attcagccag
    51661 cgccgcagtg gtttgcattt caaccatagc cattagcgtg ccccctcatt cttagccagg
    51721 cgcataatca taaacttcga tgcggcgagg acgatgaagg taatcacaaa caggatcagg
    51781 cccagttcca tcagtgcagc aacatgcaga cccgactctg cttccgcaaa ttcgttggcc
    51841 agcgcagagg tgatactgtt gcccggcata tacagcgagg cgctgtcgag ttggtaggtg
    51901 ttaccgatga taaaggtcac cgccatggtt tcaccgagcg cgcgccccag ccccaacatg
    51961 atgccgccga taacaccatt tttggtgaac ggaagaacga tacgccagat aacttcccag
    52021 gtggtgcagc caataccgta ggccgactct ttcatcatca ccggggtttg ttcgaataca
    52081 tcgcgcatta ccgccgcaat gtacggaata atcatgatgg cgaggatcac gcctgccgcg
    52141 aggataccga taccaaaggc cgggccggag aacagcgcgc cgacaatcgg gatattcgac
    52201 atgatattgc cgaccggctc ctggaagtaa acggcgaaca gcggggcgaa gataaacaag
    52261 ccccacatgc cgtaaacgat acttggaatg gcagccagca gctcaatggc gatacccagc
    52321 gggcgtttca gccagccagg cgcaagctcg gtcaggaaca gggcgatacc aaaactcacc
    52381 gggacggcga tcagcagcgc gataaacgag gtcaccagcg taccgtagat cggcaccagc
    52441 gccccgtaga tatcgttcgg cgcatcccac tctttggtcc acaggaaagc cagaccaaat
    52501 ttctgaatgc tcggccagga tgagatgatc agagagacaa taatgccacc caacatcaat
    52561 agcacagtca gcgccgccag ttttaccagc acgctgaaaa ttatgtcgcc ctttttaccc
    52621 ggtgggttaa aagcaggctt ggttgcagcc ataagttact cttcagttaa acgcgtttac
    52681 aaagtcattt tgtaatgccg gatgcggcgt aaaacaccgt acccggcctg gagttttatt
    52741 agtacagcgg cttgccgcta ctgtctttaa tattggtctt ccacgcagcg cgaacctgtt
    52801 caactacgct atccggcagg ctggcataat ccaggtcgtt cgcctgttta gccccggttt
    52861 tgtatgccca gtcgaagaac ttcagcactt cggtgccttg ttctggtttc ttctgatctt
    52921 tatggatcag aatgaacgtg gtagaggtaa taggccatgc atcttcgcct ttctggttgg
    52981 tcagatcctg agcgaatgtt ttgctccagt ctgcgccttt tgctgcatta gcgaagtttt
    53041 cttcggtcgg actcaccggt ttgccatcag cggagatcag tttggtgtac gccaggttgt
    53101 tctgcttcgc gtaagcatat tcaacgtaac caattgcacc cggcagacgc tgaacgaacg
    53161 cggcgatacc gtcgttacct ttaccgccca gaccgatcgg ccattttacg gtagagccag
    53221 taccaacgtt gtttttccac tcttcgttca ctttcgccag gtagctggtg aagacgaagg
    53281 aagtcccgga gccatctgcg cggcgtacta cagcaatgtt ctgtgaaggc agtttcagac
    53341 ccggattcag tttggcgatg gcttcatcat cccacttctt gattttgccc aggtagatgt
    53401 cgccgagggt tttaccatcc agcaccagtt cgccagactt cagccctgga atgttaaccg
    53461 ccagcaccac gccgccaatc acggtcggga actggaacag gccttcctga gccagttttt
    53521 cgtcagacag cggcgcgtca gaggcaccga aatcaacggt attagcgata atctgtttta
    53581 cgccaccgga agaaccgata ccctggtagt taactttatt accggtttct ttctggtaag
    53641 tgtcagccca tttggcatac accggcgcag ggaaggttgc acctgcacct gtcaggcttg
    53701 cttctgcaaa cacagagaaa gcactcatcg ataaggtcgc ggcgacaaca gttgcgacgg
    53761 tggtacgcat aactttcata atgtctcctg ggaggattca taaagcattg tttgttggct
    53821 acgagaagca aaataggaca aacaggtgac agttatatgt aa
//
LOCUS       DAZGTJ010000024        53099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000024 DAZGTJ010000000
VERSION     DAZGTJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.024"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..517)
                     /gene="curA"
                     /locus_tag="ACHK8E_001800"
     CDS             complement(<1..517)
                     /gene="curA"
                     /locus_tag="ACHK8E_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0344081.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKL"
     gene            698..1213
                     /gene="mddA"
                     /locus_tag="ACHK8E_001801"
     CDS             698..1213
                     /gene="mddA"
                     /locus_tag="ACHK8E_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP0344082.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1213..1662
                     /gene="ydcZ"
                     /locus_tag="ACHK8E_001802"
     CDS             1213..1662
                     /gene="ydcZ"
                     /locus_tag="ACHK8E_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0344083.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1663..1896)
                     /gene="ydcY"
                     /locus_tag="ACHK8E_001803"
     CDS             complement(1663..1896)
                     /gene="ydcY"
                     /locus_tag="ACHK8E_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP0344084.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1982..2155)
                     /gene="ortT"
                     /locus_tag="ACHK8E_001804"
     CDS             complement(1982..2155)
                     /gene="ortT"
                     /locus_tag="ACHK8E_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP0344085.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2350..2445
                     /gene="yncL"
                     /locus_tag="ACHK8E_001805"
     CDS             2350..2445
                     /gene="yncL"
                     /locus_tag="ACHK8E_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP0344086.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2542..3966)
                     /gene="patD"
                     /locus_tag="ACHK8E_001806"
     CDS             complement(2542..3966)
                     /gene="patD"
                     /locus_tag="ACHK8E_001806"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP0344087.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3988..4782)
                     /gene="ydcV"
                     /locus_tag="ACHK8E_001807"
     CDS             complement(3988..4782)
                     /gene="ydcV"
                     /locus_tag="ACHK8E_001807"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0344088.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4772..5713)
                     /gene="ydcU"
                     /locus_tag="ACHK8E_001808"
     CDS             complement(4772..5713)
                     /gene="ydcU"
                     /locus_tag="ACHK8E_001808"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0344089.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5714..6727)
                     /gene="ydcT"
                     /locus_tag="ACHK8E_001809"
     CDS             complement(5714..6727)
                     /gene="ydcT"
                     /locus_tag="ACHK8E_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0344090.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6745..7890)
                     /gene="ydcS"
                     /locus_tag="ACHK8E_001810"
     CDS             complement(6745..7890)
                     /gene="ydcS"
                     /locus_tag="ACHK8E_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP0344091.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8135..9541)
                     /gene="ydcR"
                     /locus_tag="ACHK8E_001811"
     CDS             complement(8135..9541)
                     /gene="ydcR"
                     /locus_tag="ACHK8E_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0344092.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9620..10036)
                     /gene="hicB"
                     /locus_tag="ACHK8E_001812"
     CDS             complement(9620..10036)
                     /gene="hicB"
                     /locus_tag="ACHK8E_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP0344093.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10080..10256)
                     /gene="hicA"
                     /locus_tag="ACHK8E_001813"
     CDS             complement(10080..10256)
                     /gene="hicA"
                     /locus_tag="ACHK8E_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP0344094.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10478..10708
                     /gene="yncJ"
                     /locus_tag="ACHK8E_001814"
     CDS             10478..10708
                     /gene="yncJ"
                     /locus_tag="ACHK8E_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFP0344095.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10800..12761)
                     /gene="rlhA"
                     /locus_tag="ACHK8E_001815"
     CDS             complement(10800..12761)
                     /gene="rlhA"
                     /locus_tag="ACHK8E_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP0344096.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12834..13370)
                     /gene="sutR"
                     /locus_tag="ACHK8E_001816"
     CDS             complement(12834..13370)
                     /gene="sutR"
                     /locus_tag="ACHK8E_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP0344097.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13423..14634
                     /gene="ydcO"
                     /locus_tag="ACHK8E_001817"
     CDS             13423..14634
                     /gene="ydcO"
                     /locus_tag="ACHK8E_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP0344098.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14669..15337)
                     /gene="ydcL"
                     /locus_tag="ACHK8E_001818"
     CDS             complement(14669..15337)
                     /gene="ydcL"
                     /locus_tag="ACHK8E_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0344099.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(15676..16269)
                     /gene="tehB"
                     /locus_tag="ACHK8E_001819"
     CDS             complement(15676..16269)
                     /gene="tehB"
                     /locus_tag="ACHK8E_001819"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP0344100.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(16266..17258)
                     /gene="tehA"
                     /locus_tag="ACHK8E_001820"
     CDS             complement(16266..17258)
                     /gene="tehA"
                     /locus_tag="ACHK8E_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP0344101.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            17382..18362
                     /gene="ydcK"
                     /locus_tag="ACHK8E_001821"
     CDS             17382..18362
                     /gene="ydcK"
                     /locus_tag="ACHK8E_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP0344102.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(18354..18893)
                     /gene="rimL"
                     /locus_tag="ACHK8E_001822"
     CDS             complement(18354..18893)
                     /gene="rimL"
                     /locus_tag="ACHK8E_001822"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP0344103.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(18956..19180)
                     /gene="ydcH"
                     /locus_tag="ACHK8E_001823"
     CDS             complement(18956..19180)
                     /gene="ydcH"
                     /locus_tag="ACHK8E_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP0344104.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(19320..20975)
                     /gene="opgD"
                     /locus_tag="ACHK8E_001824"
                     /gene_synonym="mdoD"
     CDS             complement(19320..20975)
                     /gene="opgD"
                     /locus_tag="ACHK8E_001824"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP0344105.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(21200..22543)
                     /gene="ydcJ"
                     /locus_tag="ACHK8E_001825"
     CDS             complement(21200..22543)
                     /gene="ydcJ"
                     /locus_tag="ACHK8E_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0344106.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            22760..23683
                     /gene="ydcI"
                     /locus_tag="ACHK8E_001826"
     CDS             22760..23683
                     /gene="ydcI"
                     /locus_tag="ACHK8E_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0344107.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(23721..25361)
                     /gene="trg"
                     /locus_tag="ACHK8E_001827"
     CDS             complement(23721..25361)
                     /gene="trg"
                     /locus_tag="ACHK8E_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP0344108.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            25760..25909
                     /gene="hokB"
                     /locus_tag="ACHK8E_001828"
     CDS             25760..25909
                     /gene="hokB"
                     /locus_tag="ACHK8E_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP0344109.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(25887..25955)
                     /gene="yncP"
                     /locus_tag="ACHK8E_001829"
     CDS             complement(25887..25955)
                     /gene="yncP"
                     /locus_tag="ACHK8E_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP0344110.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(25981..26154)
                     /locus_tag="ACHK8E_001830"
     CDS             complement(25981..26154)
                     /locus_tag="ACHK8E_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344111.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            complement(26399..26929)
                     /gene="cybB"
                     /locus_tag="ACHK8E_001831"
     CDS             complement(26399..26929)
                     /gene="cybB"
                     /locus_tag="ACHK8E_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP0344112.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            27118..28119
                     /gene="gap"
                     /locus_tag="ACHK8E_001832"
     CDS             27118..28119
                     /gene="gap"
                     /locus_tag="ACHK8E_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0344113.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(28160..29599)
                     /gene="aldA"
                     /locus_tag="ACHK8E_001833"
     CDS             complement(28160..29599)
                     /gene="aldA"
                     /locus_tag="ACHK8E_001833"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP0344114.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(29796..30596)
                     /gene="ydcF"
                     /locus_tag="ACHK8E_001834"
     CDS             complement(29796..30596)
                     /gene="ydcF"
                     /locus_tag="ACHK8E_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP0344115.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(30868..34770)
                     /gene="hrpA"
                     /locus_tag="ACHK8E_001835"
     CDS             complement(30868..34770)
                     /gene="hrpA"
                     /locus_tag="ACHK8E_001835"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP0344116.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            34971..35576
                     /gene="azoR"
                     /locus_tag="ACHK8E_001836"
     CDS             34971..35576
                     /gene="azoR"
                     /locus_tag="ACHK8E_001836"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP0344117.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(35630..36922)
                     /gene="ynbD"
                     /locus_tag="ACHK8E_001837"
                     /pseudo
     CDS             complement(35630..36922)
                     /gene="ynbD"
                     /locus_tag="ACHK8E_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(36936..38693)
                     /gene="ynbC"
                     /locus_tag="ACHK8E_001838"
     CDS             complement(36936..38693)
                     /gene="ynbC"
                     /locus_tag="ACHK8E_001838"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP0344118.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            complement(38709..39605)
                     /gene="ynbB"
                     /locus_tag="ACHK8E_001839"
     CDS             complement(38709..39605)
                     /gene="ynbB"
                     /locus_tag="ACHK8E_001839"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0344119.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(39605..40210)
                     /gene="ynbA"
                     /locus_tag="ACHK8E_001840"
     CDS             complement(39605..40210)
                     /gene="ynbA"
                     /locus_tag="ACHK8E_001840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0344120.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            complement(40379..41724)
                     /locus_tag="ACHK8E_001841"
                     /pseudo
     CDS             complement(40379..41724)
                     /locus_tag="ACHK8E_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(41788..42648)
                     /gene="pdxI"
                     /locus_tag="ACHK8E_001842"
     CDS             complement(41788..42648)
                     /gene="pdxI"
                     /locus_tag="ACHK8E_001842"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP0344121.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(42879..43469)
                     /gene="paaY"
                     /locus_tag="ACHK8E_001843"
     CDS             complement(42879..43469)
                     /gene="paaY"
                     /locus_tag="ACHK8E_001843"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP0344122.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(43451..43603)
                     /locus_tag="ACHK8E_001844"
                     /pseudo
     CDS             complement(43451..43603)
                     /locus_tag="ACHK8E_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            43604..43810
                     /locus_tag="ACHK8E_001845"
                     /pseudo
     CDS             43604..43810
                     /locus_tag="ACHK8E_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(43982..44308)
                     /gene="ydbL"
                     /locus_tag="ACHK8E_001846"
     CDS             complement(43982..44308)
                     /gene="ydbL"
                     /locus_tag="ACHK8E_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP0344123.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(44316..44501)
                     /gene="ynbE"
                     /locus_tag="ACHK8E_001847"
     CDS             complement(44316..44501)
                     /gene="ynbE"
                     /locus_tag="ACHK8E_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP0344124.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(44498..47137)
                     /gene="ydbH"
                     /locus_tag="ACHK8E_001848"
     CDS             complement(44498..47137)
                     /gene="ydbH"
                     /locus_tag="ACHK8E_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP0344125.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            47345..48334
                     /gene="ldhA"
                     /locus_tag="ACHK8E_001849"
     CDS             47345..48334
                     /gene="ldhA"
                     /locus_tag="ACHK8E_001849"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0344126.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            48445..48867
                     /gene="hslJ"
                     /locus_tag="ACHK8E_001850"
     CDS             48445..48867
                     /gene="hslJ"
                     /locus_tag="ACHK8E_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP0344127.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(48864..49130)
                     /gene="ydbJ"
                     /locus_tag="ACHK8E_001851"
     CDS             complement(48864..49130)
                     /gene="ydbJ"
                     /locus_tag="ACHK8E_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP0344128.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            49404..52928
                     /gene="nifJ"
                     /locus_tag="ACHK8E_001852"
     CDS             49404..52928
                     /gene="nifJ"
                     /locus_tag="ACHK8E_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP0344129.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        13532 a        13843 c        12565 g        13159 t
ORIGIN      
        1 taagtttgcc gatttgaccc accgtcgccc ccacaggacc tgtcgccgca gcaacaacca
       61 atgtttcgcc ctctttgggc tgaccgatat ccagcagccc catataagcg gtaaagcctg
      121 gcatccccag cacacccagc gaccacgatg gattttgcgg atgatcgcca agtttcacca
      181 gatcatcacc actggatatg tcatagtcct gccatccact gtagcccagc acccagtcgc
      241 caggctggta atcaggatga ttcgactcca caacacggct caccgtaccg ccgaccatta
      301 cgccaccaat atcaaccggc ggtgaataag atggctcatc gctcatacgt ccacgcatat
      361 acgggtccag tgacaaataa actgtgcgca gtaacacctg tccttcaccc ggtgtggcga
      421 tatcatcttc ttcaagacgg aaattctccg gaaccggcgc gccatgcgga cgcgaggcca
      481 gaacccaacg tcgattacgc tgcttttgtt gccccataat gctctcctgt gcttttggca
      541 tgaatccatc aatagtagca ggctcgcagg aatggttttg tttgcagccg tcacatcccc
      601 tctgcaacac gcaataattt catcgaggaa tccgttgctt cgggctgttt tcgatagtat
      661 tgataacgtc atcatcatta tttaatcgag gccaaacatg tccatccgtt ttgcccgcaa
      721 agccgactgt gctgccattg cggaaattta taaccacgcc gtgttgtata cggcggctat
      781 ctggaatgac caaacggtgg atgcagataa ccgcattgcc tggtttgaag cgcggaccat
      841 agcaggttat ccagtgctgg tgagcgaaga agatggcgta gtgacgggat atgcctcgtt
      901 tggcgactgg cgtagtttcg atggttttcg ccataccgtg gaacattcgg tttatgtcca
      961 tcccgatcat cagggcaaag gtctggggcg taaattgtta agccgattga ttgatgaagc
     1021 gcgggattgc gggaagcatg tcatggtcgc cgggatcgaa tcgcaaaatc aggcgtcttt
     1081 gcatctgcac cagtcgctgg gatttgtcgt caccgcgcaa atgccgcagg tgggcactaa
     1141 atttgggcgc tggctggacc tgacatttat gcagttgcaa ctcgacgaac gcagtgaacc
     1201 ggacgcaatc tgatgaatca gtcgctcacc cttgcttttt tgattgccgc cgggattggt
     1261 ctggtagtcc agaacacgct gatggtgcgc atcacccaga cctcctctac cattctcatc
     1321 gccatgttgc tgaactcttt ggtaggaatt gtgctctttg tttctatttt atggtttaaa
     1381 caaggtatgg cggggtttgg cgaactggtg tccaacgtgc gctggtggac acttatacct
     1441 ggcttgctgg gatcgttttt tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg
     1501 gcgacaacta ttgcggtact ggtcgccagc cagctgattg gcggtctggt gctggatatc
     1561 ttccgcagcc atggcgtacc actacgggcg ttgatcggcc caatttgtgg cgcaatttta
     1621 cttgtggttg gtgcctggct ggtggccagg cgcacatttt aatcagagga tggtgccacc
     1681 tttggttaac tgttcgtggc gcgcttccat atcttcttta tgtttgcgac cgtgatgggc
     1741 gatcgctgtg cgaagacgct gctgctgggt atagcgttct tcccgactta actccgcatc
     1801 atcgctcagg gcgattaata attcgttcat atgggcaatg acgctctctt caatcgcggc
     1861 atccacgcgc gcgatgactt catccagatg cgacatactt tctccttagc cacttgtgaa
     1921 atttatcgat tcatccaaat gatataagtc tgcgtggtgc cgtcaagcca gtgagtaaga
     1981 tttaaaacag tgaaaacaac agcgccaccg ggaaactcat cggccatgtt gcccccacca
     2041 gaaaggcgct taagaaacgg atgcgtttct tatcgtgaga aagaaaccag gtgataagaa
     2101 atgcgattgc tgccataacc gcataaaaaa caagcatgtg ttgatagaga gacatgtacg
     2161 ggcgtccagc cagatgaata aaacgcgcgc aaatatgcgt cattttttga cgtacatcaa
     2221 gttttttaac cggtttgaga aaaaaacatc aaacagcaac ttgcccttat ccatcttcag
     2281 aatgagtatt attgcggatt ttcttaaccc gtactataca cataggggca atgataaaag
     2341 gtggcaaaaa tgaatgtttc cagtagaact gtagtactga taaatgtctt tgctgctgtt
     2401 ggtttgttta ctcttatctc tatgagattt ggctggttta tttgatgtag tgattttgca
     2461 cgcagcacgt actgtcagag aaatcagtgc tctgacagta cgcaagatca tctcatactc
     2521 ttacgatctg caatattatc cttaatgttt aaccatgacg tggcggacga cggtgtaatc
     2581 ctccagccca taaagtgaca tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg
     2641 catttcactt accagcatga aatgggtatt gacccaggta caaccatatt gcagccgtgc
     2701 gctgacgcga tgcgccctgc ccacatcttt cgtccatacc gaagatgcaa gtccgtactg
     2761 gctgtcattc gcccagttta ccacctgttc ttcgttgtcg aagagtgtaa cactcactac
     2821 tggaccaaat acctcttttt gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt
     2881 cggcgcatag taatagccat tacccttgcg cttttcaccg ccagtgatca ctttgatgtg
     2941 ccctgtcgct ttcgcctctt ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct
     3001 taaaggtcca agctccgtag actcgtcatc cggcgcacca gattttaacg ttgccaccgc
     3061 agcacccagt ttttccacca gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc
     3121 cgcagtacaa tcctgtccgg cattgtaata gccaaatgta cgtacacctt cgaccactgc
     3181 ctcaatatcc gcatcatcaa aaacaatcac tggcgctttg ccaccaagtt ccatatgagt
     3241 acgctttatg gacggcgcgg tatggctgat gatgtgttcc ccagtggcga tagagcccgt
     3301 cagcgacacc atccgcactt tgggatgacc ggtcagcgga tcgcccaccg ttttgcctct
     3361 gccaaacagt acattaatca cgccagccgg gaagatatct ttcgccaact ctgccaactt
     3421 caacgctgtc agcggggtaa tttctgatgg tttaagcact acgcagttcc ctgccgccag
     3481 cgccggagcc agtttccacg cagccatcat cagaggataa ttccacggtg cgatagaagc
     3541 cacgaccccc aacggatcgc gacggatcat cgaagtatga ccttcaagat attcacctgc
     3601 cgccagacca ttcagacagc gcgccgcacc agcgaaaaag cgaaaaacat cgacaatcgc
     3661 cgggatttca tcattgaacg cactatgcag cggtttgcca caattacggg actccagttc
     3721 ggcaaaaacc tgaccatttt cttcgataac atcagccagt ttcagcagac attccgcacg
     3781 cactttcggc gtggtttgcc cccattcggc aaatgctgca tctgccgcgc gaacagcagc
     3841 atcgacctgc tctgcggatg cctcggcaat ctccagtaaa acgtcccccg ttgccggatt
     3901 atagacaggc tgtttttccc cttcgccgct aaccagttct ccgttaatca gtaacttatg
     3961 ttgcatagca tattcctgta tcagtggtta ttgaccattg tcgccttcgc gggttagcca
     4021 ccaggccccc aggatcggca aggttgttac caacataacc agcagtgcca ccacgttagt
     4081 taccggtaca tcacgcggtc gcccaagctg attgagcaac cacaacggta acgttcgttc
     4141 atgacctgcc gtaaaggtcg taacgatgat ttcatcgaac gacaaggcaa acgccagcat
     4201 tcctcctgcc agtaacgccg aactgagatt cggcaacact acgtagcgga aggtttgcca
     4261 gccattagcc ccaagatcca ttgacgcctc aaccagactc cacgaggtgc ggcgaaaacg
     4321 ggcgatgaca ttgttaaaga ccaccactac acaaaaagtc gcatgaccga ccacgatggt
     4381 gaaaaatccc ggctccagat tgatggtttt aaaggcggtt aataacgcca gaccagtgac
     4441 aatgcccggc agcgcaatgg gcagcagcag taacagcgaa atggcgtttt tgccgaaaaa
     4501 gtctcgtcgc cacagcgcgg cagcggccag cgtccctaac accagcgcga ttaatgtcgc
     4561 cagcgccgcc actttaagtg acagtgtcac ggcatcaaga atatcactac gctgtgctgc
     4621 cacgctaaac cagcgcagcg tcaggccttg cggtggaaaa ctaaacgccg catcttcagt
     4681 gttaaacgca taaacggcga taatcagaat gggaaaatgt aagaaaacca cgccgcccca
     4741 ggccgccagt ttgaggaaaa acggtgcgcg ttcagagtgc atcgaacgct cccagacgtt
     4801 tcacgaacgc taggtaaagt gcgatgagta caatcggcac tagggtgaat gccgccgcca
     4861 tcggcatatt gccaatcgcc ccctgctggg aataaaccat attgccgata aaatatcctg
     4921 gagggccaac cagctgcggg acaataaaat cgcccagcgt gagtgagaag gtaaagatag
     4981 agccagcggc aatacccggg attgccagcg gcagcaccac atagcgaaag gtttgtcgtg
     5041 gacgtgcgcc gagatcagct gacgcctgca acaatgacgg cggcaaacgc tcaagcgccg
     5101 cctgcaccgg gaggatcatg aacggcaacc agatatagag aaacaccaga aagcgcccca
     5161 gcccggaagt tgacagcgta ttacctccca ccgccggtag cgttaggaac gcagtcagca
     5221 gtggttccag ccccagatgt tgtaaaaacc actgcgccac gccatctttt gccagcaata
     5281 acgtccaggc ataggctttc acaatgtagc tcgcccacat cggcaacatt accgcgatat
     5341 aaaaaaacgc tttcattttc ccgctggtat agcgcgccat ataccacgcc attggaaaag
     5401 ccagaatggc gctggcgata gtgactgcca cagccataat cagcgtgcgg agaatgatgt
     5461 cgtaattcgc cggattaaac agcgcacgga tattcgccag cgtcagttcc ggcgttaccg
     5521 acatggtgaa atcgtcaaag gtataaaatc cctgccataa cagtgtcagc agcgagccga
     5581 aatagacaat gccaaaccac attagcgggc caagcagcag taaaaacagc cccagccccg
     5641 gattacccca gaagaaaccg gacaccttac ccagacctgg acgtgaaggt gattgcaata
     5701 cgttcatcgc cattcacctc tcctcaacca gcggtaccat cacatcacgc gaccaggaaa
     5761 ccatcacctg ttgtccgggc gtgagcgtgg cgggcagttc ttcgcctgtc atattggcct
     5821 gactcaccag cagtttttca ccgccgttca gtctcagttc aaaacgggtc gccgcgccct
     5881 gatactgcac cgcctggatc gtgccattag cctgcacttc gccggggatg ttgaagcgga
     5941 tatgttccgg tcgcagggcg aagcttcccg tcatgccaca aagtttgtct gccatcagtg
     6001 catcaaaaac attcgatgtc ccgacgaacc cggcaacaaa cggcgtgcgc gggcgcatat
     6061 agagatcacg aggggaatcg acctgctcaa tgcgtccatt attgaaaacc gccacacgat
     6121 cggacatcga taacgcttcg ccctgatcgt gggtaacgaa gataaaagtg ataccgagag
     6181 actgttgcag ttttttcagt tccagctgca tctgctcacg caatttgaga tccagtgcgc
     6241 cgagcggttc atccaacagc aatacgcgcg gttcattcac caatgctctg gcgatagcaa
     6301 cccgctggcg ctgaccacca gaaagctgtg acggcttacg ttgctgaaca aaaccaagcg
     6361 ccactttctc cagcgcttct tgcgccatag catatcgctg ttttttatcc acgcctttga
     6421 ccatcagccc ataggccacg ttgtcaagaa tcgacatatg cggaaaaagt gcgtagtcct
     6481 ggaagacggt attaacatcc cgctcccacg gtggcagatt gctggcggat ttaccaaaga
     6541 tagagatagc cccgccggaa agctgttcga agccagcaat caggcgcagg caggtggttt
     6601 tgccggagcc ggacggcccc agcatagaga agaactcacc atcttttatc gcaatactga
     6661 cgccatctac tgcgcgcacg tcaccgtaca accgtgagac gttgtcaaac tccactgcgt
     6721 acgtcataaa acaccccagc gaagttagcg accgcccata atggcaatgt aatcctgcgt
     6781 ccagcgactg tagggaacaa acttgccccc ttctgctata ggcgttttcc agaaggcgat
     6841 tttatcgaaa tagttaaaac cgttggtttc acaacctttt tcacctaata acggactggc
     6901 tttacaccct tgcggcacta ctggtaacga gccaaaccag gccgccacat caccctgcac
     6961 ttttggcgtt aatgaccagc tcatccattt gtaggcgcaa accggatgtt tcgcttcgct
     7021 atgtagcatg gtggtgtcag cccaaccggt aacgccctcc ttcgggaaaa cggtagcgac
     7081 aggctggcct tcggctttca gggcgttggc ctgatagggc catgcactgg aagcaaccac
     7141 accctcgttt ttgaaatcgc tcatttgcac ggtagtgtca tgccagtagc gatggatcaa
     7201 actatgttga tcgcgcatca ctttcagtac cgcctgatac tgttcttcgg tgagttgata
     7261 cggatcgcgg atgcccaact gcggctgagt ggctttaacg aacaacgcag cgtccgcaat
     7321 gtagataggg ccatcataag cctgaacgcg gcctttattg ctcttgccgt ccggcagatt
     7381 ctgctcaaca aaaaccactt gccagctatc cggcggcgtc gggaaggttt tagtgttgta
     7441 catcagcagg ttcggccccc attggtaagg tgtgccgtaa actttgccgc caacgttaaa
     7501 ccagtcgcct ttaaccacgc gcggatcgag cgctttccag ttgggaatca aggcggtatt
     7561 aattggctgc acgcgtttac ccataatcaa acgcagcgag gcatcgccgg atgccgtaac
     7621 cagatcgtaa ccccctttgg ccatcagact gaccatttca tcggaagtcg cggcggtttt
     7681 aacattcacc gcgcagcctg tctctttttc gaattgcgtt acccagtcgt attgtttatc
     7741 agtttgtccg cgttcgatgt atcccggcca ggcgataata tccagtcgcc cttccggttt
     7801 atctaaattg gtaggtggtt cggcggcgtg agcggtcatt attgtcatgg tgagcgcaca
     7861 caggctgctg cgggcaaatg tcttgctcat aaagtcttac tcctgtcgta attaattgtt
     7921 cagccgccgt gccgtaaaaa tatctccttg tttaaccata gacggcggct agcaggcaca
     7981 ccttcgcccc tgagaaaatt taatcaggtt gtgagctacc gcgcattatg ccgtatgtaa
     8041 ggttatcttc tggagtatag acaaggagtt aggaaatatg actgctattc gaaattcata
     8101 ttacccaacc tgagaggaat ttaattaaac gctattacag ccgttcttga ataagtttgc
     8161 ctaattgttt aaccgcctgt tcttcacgtt ctccccactg ccacgcggta ttaaaacgga
     8221 aaaaacgtga ccagttttca ccggtagaaa acattttacc cggcgcaata ctgatatgat
     8281 gcgtcagtgc tgccaggctt aattcgcccg catctaacgg ctcggggagc tccagccaga
     8341 gaaaataacc gctgtcatta tgatgaattt tcacttccgc aggcagataa cgcagcagtg
     8401 cttgccaggc acgttgttta cgttccgcaa gctggcgacg caggcgacga agatgggcgt
     8461 cgtatcggcg cgtggaaagg taatccacca gcgcaagctg catcggtgag ctggtggaaa
     8521 gcgtactcat caactgcaag cgttgaattt tacgtgcatg ttttccggcg gcgacccaac
     8581 caatacgaaa accaggcacc agacatttcg aaaacgaaga gcaatgcaaa acaccatcgt
     8641 ggcgatccca cgctttcgca ggcagcggtt tttcccgtcc aaaataaagt tcgctgtaaa
     8701 cgtcatcttc aatcagcgtt acgttgtact gattgagcaa cgctaccagc cgcgcttttt
     8761 tctgcggcgt taaggtaaat ccgagtggat tctggctatt agtcatcagc cagcacgctt
     8821 tcaccggata ctcctgcaac gccagttcca gcgccttgag atcaatccct tctttaacgt
     8881 ccgtcgccac cgataacgcc ttcagccgta accgctccag cgcctgcaac gcaccgtaga
     8941 aacaaggatt ctctactatc acccaatcgc ccggttcagt gaccgcttgt aaactgaggt
     9001 ttaatgcctc taacgccccg gcagtaatga caatttcatc gggtgaaatg gtgatgccct
     9061 gtaaggcata gcgacgagct atagcctgac gcagttcagc gtttcctggc ggtaagtttt
     9121 caatcacgct catcgccgtg gcggttttgc ttacctgtgc cagcgagcgg ttgagttgtt
     9181 ggagggggaa aagtcgcggg tcgggaaagg ccgaggcaaa cggaactacc gacggatcgc
     9241 gactggcctg caacatatca aaaatatagg tgttgatatc gactgcttca tctcgagtga
     9301 ctggaatgac tggcgctttc ggcattttta ttgcctgtgt cgcaacgtaa taacccgact
     9361 gcggtcgcgc gataatatgt ccctgacttt cgagcaactg ataggcatgg ctgacagtca
     9421 taaagctcat gccagaaagc gccacctggt cacgcaacga aggcaaacga tcgccaggtt
     9481 gccagatacc cgacgcaatc tgctcgcgta attgttctgc aagctgctgg tattttttca
     9541 ttatcgaatc gtaattatgt gcagatgatt cggcagtcta tatcagttgt taaaaatgac
     9601 aacttttcat taactgttat caaaccatca ccagtgataa ctctttgcca agcgccttag
     9661 ccgcaagctg gacggcgtcg atttttgtag catggtgcaa gttaaataag cgagtaatct
     9721 cctgtttagg tttgccaatt cgtctggcta actcttgctg agtgatttct gactgtaaaa
     9781 aagcatttaa cagcaatact ttagaggcga tacttaaagg cacttcaata aagtgatcgt
     9841 tgttagttaa tggcgaaggc aacgggatta gctcattatc ttcaaaataa aaatcaaatg
     9901 cggtgagtaa ggcatctttt gctgcttcca tcgcttcagc aacagtttct ccctgcgtca
     9961 acgcttcagg gatatctaca aaagaaacca cataaccgcc ttccggcgca ggagtaagag
    10021 tgacgggata acgcataatt ttgactgtct ctcgcatgtc attaaccttc ttatggcgat
    10081 taatttaaac cgagttgctt caggattgct ttacgcaatg gttcttttat ctcatcactg
    10141 gggtgacgcg gcatgacact gcgtcgccct tgaaacctga gtttcaaatg gttgctgcca
    10201 ttcgccacat cgacgccctg agattcgagc caacgtctga actcgctttg tttcacatcg
    10261 cctccctgcc gctgagtata tcaacaaagt aaacattagt gtttacctcc gtgtgtaatt
    10321 tgctcgaaaa tagcactaaa tcgccgcaac ggcagaatgc aatgcaaaat caggaaggaa
    10381 gattccagaa tttctttgcg ctaatttgca aaactgaata ataacctcaa gaaacgatcg
    10441 gcatcatagc gacaaaaggt aagaaaaagg tggcaggatg tttacgaagg cgttatcggt
    10501 tgtcttatta acgtgtgcac tgttctcagg acaactcatg gcagggcaca aaggacatga
    10561 atttatgtgg gtaaagaatg tggatcatca gctgcgtcat gaagcggaca gcgatgaatt
    10621 gcgtgctgtg gcggaagagt cggcggaagg tttgcgcgag catttttact ggcaaaaatc
    10681 gcgcaaacca gaagcgggac atcgttgagt catttgttgc cggatgcggc atgacgcctt
    10741 acccggcagg caatatcatg cagatttgat gagttgtgag taaccagaaa actggcgttt
    10801 tacccttttc gcttcggtaa agttttcagc agctcatccg gacttacgga tgccactacg
    10861 cttcccggta acggcatttt cagtatgtga tttttgattt tgccaatgac gtgcatctcg
    10921 catgggcggc aatcaaactt tagcgttaat acttcatcgc cgttaaccag ttgcattggc
    10981 gtcgccttcc agcttttgat attgcctttc gcctgtttcg ggcacagatt gaaggcaaag
    11041 cgcagacaat gcttagtgat catcaccggg acttcgccct tctcttcatg cgcttcatac
    11101 gccgcatcaa tcagttgcac accatagcga tgataaaatt cacgcgcttt ctggttgtaa
    11161 acgttcgcga ggaaactcaa atgcgtttgc ggataaaccg gcgcaggatc agcaaccggt
    11221 ttacggctgc cgcgctggta actggcaaga cgcgcagcat ctagcatgtc ggcggcttca
    11281 cggcggaact ggtttaacag actgtttggt acaaacagcg cacccggcaa attaatttgc
    11341 acatcgcggg cgtaatagat ggtttgcccc agttttgcca gaccatcctt tagattgttc
    11401 attgcttttt cggcgttatt ggcttcgtcg aactgcccgt ccagcgtatg cgtgatgctg
    11461 acaccctctt cactggtgag ggtcagaatc agttgttcct gccagccgcc cagttcaatg
    11521 tctaccgcca cccgacgttc gctggaggtt tttgtcagtg cctgctgcca gttatgatca
    11581 agattacggt ttagtgggtg atgcgggcga attttgtgca actctgctgg catttcattg
    11641 ggccagacgc ggtactgatt ttctccggtt ttctcgaccg tattggcacg aaagccgacg
    11701 acttcacgtt taatcatcac gttcaggcca tcgccatttg ccagtggctc ggtaacggca
    11761 acatcgagat gatctttcgc cactttcaat acttcaccca ccggcaggcc gataaatttc
    11821 ggcgaatcga acgcgccaat atcgccttta cgggcattca caaaataatc tgtgctacca
    11881 cggtggaaag tcttttccgt cgatggaaca aagaaatgtt cagtacgacc tgatgaagca
    11941 cgcgtcagat cgccacgttc ttcaataatg gcatccagca tctggcgata atgggcggtg
    12001 atattcttca cgtagctcat atctttgtaa cgcccttcaa tcttgaagga gcgtacgcca
    12061 gcatcaatca gcgcgccgag gttggcagtc tgatcgttat ctttcatcga cagcagatgt
    12121 ttttcatagg aaaccacccg cccctgatcg tctttcaatg tgtatggcaa acggcacgcc
    12181 tgcgagcaat cgccacggtt ggcgctacgc cctgtttgcg catgagaaat gtagcactga
    12241 cccgaatagg ccacgcacag tgcgccatga ataaagaatt caatggtcgc gtccgtggcc
    12301 tggtgaatcg cgcggatctg ctcaagattc agctctcgcg ccagcacaat ctgcgtgaag
    12361 ccaacatcag agaggaactt cgctttttct actgtacgaa tgtcgcactg cgtactggcg
    12421 tgcagttcaa tcggcggaat atcaagttcc agaatcccca tatcctgaac aatcagcgcg
    12481 tcgacgccgg tctggtagag gtcggtaatc agccgttgcg caggttccag ctcatcatca
    12541 tgcaaaatgg tgttaagcgt gacgaaaatt tttgcaccat aacgatgggc aaatggcacc
    12601 agttcggcaa tatctttcag gctattactg gcattatgac gggcaccaaa accagggccg
    12661 ccgatataaa cagcatcggc accgtgcaaa atagcttcgc gggcaatggc ggcatcgcgt
    12721 gccgggctta acagttcaag tcgatgagaa gatacggtca cattcttatc cagtagctga
    12781 aaaatggcgg ctattttagc cacaagcgcg acgttgcgaa atagttttcc cgcttaactg
    12841 cgtgggtaat ggatgaggga atgaaaatgt acgatttgct ccccaccatt acgatagaca
    12901 tgcgtaacgt ctgcagcaaa tcgagcgcct tctccacaat taaggctctg ccattcacca
    12961 tcaacgcaca gatcaagttg cccatcaatg acgacaacat gttcgattac tcccttctca
    13021 tggggcgtcg attcacttat tgcaccaggt gccatctgaa tcgagaagtg ttcaaagcag
    13081 agttgtggat cataaggaaa cagcgaagtg atcaccatca cctgctgttg cggatcataa
    13141 actgaaggcg tagcggactg cggcggagaa ataaatgtcg aaaatggcac attcaacccg
    13201 gtggcaattt tccataatgt cgcgaccgtc gggctggact cattacgctc aatctgccct
    13261 aacatcgctt tagaaacgcc cgtcgcttct gccagtcgcg aaagactcca gccgcgctgc
    13321 tgacgtagtt gtttaagtgt ggtggataaa aagcgagtga gattttccat gcgactcctc
    13381 cggcaaaacg gaagtttatc acttgtgcgt tataacggac aaatgctacg gtacctgtat
    13441 gctataacgc acgaggtgac catgcgtctg ttttctattc ctccacccac gctactggcg
    13501 gggtttctgg cggtattaat tggctacgcc agttcagcgg caataatctg gcaagcagcg
    13561 attgtcgccg gagccaccac tgcacaaatc tctggctgga tgacggcgct ggggctggca
    13621 atgggcgtca gtacgctggc tctgacatta tggtatcgcg tacctgttct caccgcatgg
    13681 tcaacgcctg gcgcggcttt gttggtcacc ggattacagg gactaacact taacgaagcc
    13741 atcggcgttt ttattgtcac caacgcgtta atagtcctct gcggcataac gggactcttt
    13801 gctcgtctga tgcgcattat tccgcactcg cttgcggcgg caatgcttgc cgggatttta
    13861 ttacgctttg gtttacaggc gtttgccagt ctggatgatc aatttacgtt gtgtggcagt
    13921 atgttgctgg tatggctggc aaccaaggcc gttgcaccgc gctatgcggt aattgccgcg
    13981 atgattattg gggtcgtgat cgttatcgcg caaggtgacg ttgtcacaac tgatgttgtc
    14041 tttaaacccg ttctccccac ttatattagc cctgattttt cgtttgctca cagcctgagc
    14101 gttgcactcc ccctttttct ggtgacgatg gcatcgcaaa acgcaccggg tatcgcagca
    14161 atgaaagcag ctggatattc ggctcctgtt tcgccattaa ttgtgtttac tggattgctg
    14221 gcactgtttt tttccccttt cggcgtttat tccgtcggta ttgcggcaat caccgcggct
    14281 atttgccaaa gcccggaagc gcatccggat aaagatcaac gttggctggc cgctgccgtt
    14341 gccggcattt tttatttgat cgcaggtctg tttggtagtg ccattaccgg gatgatggct
    14401 gccctgcccg taagttggat ccagatgctg gcaggtcttg cgctgttgag taccattggc
    14461 ggcagtttgt atcaggcgct gcataatgag cgtgagcgcg acgcggcggt ggtggcattt
    14521 ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttctg gggattaatt
    14581 gccggaggcg tttgttacgt ggtgttgaat ttaatcgcta acagaaaccg ataataaaaa
    14641 gcgccatcta acgatggcgc aactggtatt actttttgtt aacgtcaaac atggtggcat
    14701 cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt
    14761 tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg
    14821 catcaatcag ctcaccttca aaatacaggc gagtatccat ggtacggtgg cccgtcgcca
    14881 tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt
    14941 tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg
    15001 taaccagtgg tttacgctgc gcaatcgctt ctttcatttc ggtgttggta taactcaaaa
    15061 ttttatccag aactttctgc cctacctggg tagatggttt cggtaccgga taataagtga
    15121 ctgggttcca gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta
    15181 aaacaggttt acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac
    15241 cagaatattt atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc
    15301 ataaagctgc aactttcgca attgatgtgg tacgcatgaa tagtttcctg agatgattat
    15361 attactttct aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa aagtgttggt
    15421 gaaaataaat tttttagccc cttttcagac gacatagtca atgccgaaaa agacatagtc
    15481 aatgccgaaa aaggggctgc cgaaaaaggg gctaagtcaa tcatcagtag ttaaatttca
    15541 gtaagttaag ctgctaaaaa atttcgacta ctgtgattaa tttcacatcc attcgataaa
    15601 aaatatgctt accgaaaaga agaacagtaa accatctcag cgtggggaat tttcacgcgc
    15661 cttgttccta gcgggtcatt ttttacgtgc cagcatcgtg gcgaaacgca gtttaatacg
    15721 attaccgttg gcgtcggtgc ggtgcagctc gccgacgtct tcattgtatt tcaccatctc
    15781 ccagccttcg taatatcgac gtaattcccc ctctttgaag gcaaacggga agccgacggt
    15841 acatggataa tcagcggtat ccatcgccgc cacaatcagg ttgtaaccgc caggtttagt
    15901 gcaacgttgc atattggcaa tcagccctgg gatggtttta gcctcaagga acatcagcac
    15961 cacagtcgaa agaataaaat catactgtcc atcaaatgtg aggttattca gatcaacgac
    16021 ccgggtgtgt aaattctcca gattttcaat ggatttaatc cgctcgacgt tggcgatact
    16081 catggcattt ttatcccatg cgtcaacatc ataaccattg gctgccaggt aaagactgtt
    16141 acgaccattg ccacagccca gatccagcgt tttacccggt ttaaccactt tcaccgcttc
    16201 cagcacttca gagtgtgtgc gagttaattc atatttatca gtaaaatagt tttcgtcacg
    16261 aatgatcatt ctttgtcctc tgctttcatt aaaacggcgc gctcggttct gattaacaat
    16321 tttccctgca tcagaagcgc aaaagtacgg atgagcagta ttgcaataat aaaattggta
    16381 aagataaaca gcggcaccgc cagcgtatgg aaaaatccat tatcgctgcc actccccaga
    16441 tgcaaaccgg tggttgccag tgcagatacg ccgaacgaga aactccagaa tgaagcatta
    16501 aatggctggg agagatacca tggcatcaga cgtagcataa acagcagttg cagcagtcca
    16561 taaccgaaaa gcattttcgc cagcgtgtca ccctcgccgc cgttgacgct cagccaggca
    16621 ctacaggcca ccagcgcagg agcgagctga atgccgagtg atgtccgcag cgccgtgggt
    16681 aattctcccg aactgcgcag acgctgcaag atcacaggtt caaggcttag ccatgagaaa
    16741 acgcctgcgc ctaaaaacac cagaccggcg tcggtgtagc ccaacgcacc acaggccatt
    16801 gcgctgataa agttgttggc aactgtcggc agatacagtc caggcgtggt agcttcttca
    16861 gggtgagatc cgcgccataa tcccgcagtt tgccaggcgg cataagccaa ctgaaccacg
    16921 acgccaaaac tgaacaggca caccgccagt gggcgaaacc acggaacaaa accaatcgcc
    16981 accagcatcg tcgttgccgg aaacaaactc acaaaactgc tcagcactgg atggcgcact
    17041 tccgccagca cgctatgcgg aaagcgtatg agtcgggtaa taaatgcgct agttaataat
    17101 ccccagatga tcatcgccag aatcaccagc ccatccccta accagtggct gacctgccaa
    17161 acctggctgg catagcgcca ggcaaatccc atccctatcg tccccaacac aataccaaag
    17221 tagcctgccg gcaaattgag cactttatcg ctctgcatct tcttactcat tttgtttaat
    17281 ttataaagta tatttgaaat gcattttatg gaattttctc gagcatagcc agagccgcag
    17341 aatttgctac ggttatagta agtcacctgt gggaaaacgc tatgcgcaaa tatcgtctaa
    17401 gtgaagaaca gcgagccttt agttatcagg atgatggcac taaaaaaagt gtgttattac
    17461 ggcagattat cgccatgagc gattttaacg atgtgatagc aggaaccgct ggcggctgga
    17521 tcgattgcga aacggtgtta gcacaggaag gaaattgctg gatttacgac cagaatgcta
    17581 ttgcgttcgg cggagcggtg atttccggta atacgcgcat caccggcacc agcgtactgt
    17641 ggggagaggt ttacgcaacg gataacgtct ggatcgataa tagtgaaatc agtcagggtg
    17701 catatataag tgacagtgtc accattcgtg actcattagt ttgcgggcaa tgtagaatat
    17761 ttggtcacgc cttaattgat caacattcta tgatcgtcgc cgcacaaggc ttaacgcccg
    17821 accatcaact cctgctacaa atttatgatc gcgcaagggt tagcgcctcg cgtattgttc
    17881 accaggcaca aatttatggc gatgccgtcg tccggtatgc ttttattgaa catcgcgccg
    17941 aagtttttga ttttgccagc gttgaaggta atgaagagaa caacgtctgg ctatgcgatt
    18001 gtgctaaagt ctacggtcac gcgcaggtga aagctggcat agaagaagat gccattccga
    18061 cgattcatta cagttcgcag gtggcggaat atgccattgt ggaaggtaat tgtgtgttaa
    18121 aacatcatgt cttgattggc ggaaatgccg tggtacgtgg cggaccgatt ctgctcgacg
    18181 agcacgttgt aatacaaggt gagagccgta ttacaggtgc ggtgataatt gaaaaacatg
    18241 tggagttaac cgaccacgcc gtcgttgaag cctttgatgg cgacacggtt catgtccgtg
    18301 gtccaaaagt cattaacggc gaggagcgca ttacgcgaac gccgctggca gggttattgt
    18361 gaatcgataa tacgcgcgta taagttcaca tcatcatagg catcattcag gaactcagcc
    18421 tgtttcagac aaccttcaag gataaaacca ttgcgcaaag cgacctggtt gctttgcgca
    18481 ttgtccaccc gacatttgat cacgaagcgt ctaagttcac cagactgagc gtaatgatga
    18541 atcaatgcct gcagcgcctg agaaatgatc ccctgcccct gatgagattc gtccagccag
    18601 tagccgattt cagcggtttt attcagtggt tcaatacgat taaacgagat aacgccgata
    18661 agttcatctt ctttgaaaat catgaacatt ttggcatagc cgcgttgatg caacatcaca
    18721 ttaccctgca ccgtttttcg cgtgtcctct tcactttgaa caaactgcgg ccagtttagc
    18781 gactgctgta accaggttct atttttacag attaactgat aaagaggtgt gacatgactt
    18841 tctgcaacag catgtaattc aagtgattca cttactttta tcgtttcagt catcttatct
    18901 ccagtcaatc catgcagttg ttaaaaaagc cgcctggctg gcggcttagg aaagtttaca
    18961 cctctttgac gctcacctgc tgcaggattt tgagcatctc atcttttaac tgtagctttt
    19021 gttttttcat gcggaccact tccgcattgt accctcgacc gtcggaacct tcctttctgg
    19081 caatttcatg atcaagttta ttgtgtttat cgaacaagga cataaagcga ggattttcgt
    19141 ttttcagacg ggatattaaa tctcggtatt ctggaaacat acgcactccc tgttagtggc
    19201 tgacaaggtg tatctgaaat acacaccatc agattactca tttaacttag ggaaaaatgc
    19261 gagcgggatc gtgtttaacc agacggcacc tcccgaagaa gatgccgaag aaaaaacgat
    19321 taactcatca cgcggtcatc aacatactga cgtttatccg gcgctggcgg gaaatactga
    19381 tacagccatg tttcactgat agcgtcgccc tgacaacgca gatacatccg catatcgacc
    19441 ggatcagtgg agtccgaagt cggataccag tcaaactgaa tacgataacc atcaatgggt
    19501 tcaatataga gaatttcgat ttgtttcgct tccccactgg aaagcgtaat caccggctca
    19561 atgccttttg gcgcggcagc tttcagatca ccaccaacga aatcgacggc aaaacgtcgc
    19621 gcccattttt cgggatagtg ttcacctggc gcccaacctt ccgggaaacc gcccatgccg
    19681 gtacgcgtcg ccataacgcg cgctaatggg caatgaacag gcggttgcgc actccagtac
    19741 agacgatact ggaatgcaaa ctcatcaccc gcttttaccg ctttttctgg ctgccagaag
    19801 cagacaatgt tatccagcgt ttcgcccgtt gttgggattt ccatcaggcc gatggtgccc
    19861 ttaccccact tgttacgcgg ctccacccac agacttgggc gtttgttata ccagcccata
    19921 atgtcctgat aatgggagaa atcacgatcc agttgcaata aaccaaaccc tttcgggttg
    19981 ttgtcggtgt aagcattgaa ctgcaatttt tgcggattat tcagcggacg acaaatccac
    20041 tcgccattgc cccgccacat ggacaaacga tcagagtcat ggatttgcgg atgaatggtg
    20101 tcgcacatcc gacgttcatt agtaccgcag ctgaacatgc tggtcatcgg cgcaatgccc
    20161 agctgtttaa tgtctttgcg cgcatacagg tgattttcca catccataat cacctgactt
    20221 ttctcacaat ggatagtgaa cttataggca ccagtaatgc tggcgctatc gagcaacgca
    20281 taaacggtaa aggtggttgc ccccggtttt accgtatcaa accagaagtc agtaaagtcg
    20341 gggaactctt ctttactgtc ggtgtaagtg tcgatcgcca ggccgcgggc cgacaaaccg
    20401 tattgatatg tatcatcaac cgcgcggaaa taactcgcgc cgaggaatga tacgacatca
    20461 cggcgcgcca gttcgggggc tttaaacacg cgaaaaccgg caaaaccgag atcactttgt
    20521 ccttctaatt gtttggtatc aacacccgca tcgttgtatt tgaacaactc cgggcgaaag
    20581 tgaatttcac gcgccagatg tgttgctgga tctacagaaa acatacgaac gcggcgacgg
    20641 aatcccattc ccatatggaa gaactgagcg tccagttgac ggttctcaac gttatgccag
    20701 agcgattttt cggcgtcgta ttgaatactg ttataagcct gcggcgtcat tgtcgccaga
    20761 gtgtcaggta acggacgcgg cgcaccacgc cacgctgttt gcgctaagtc gtgcgccatt
    20821 gactgtagaa tggagaagtc aaaacgttgg gtttgcccgt cggcaatatc agaatctgcc
    20881 gcgaatgccg cctgagaaaa aagagaagca atgccgctgg taccgcacac ggcggccata
    20941 gccattgaac ctttaataaa tcgtctacga tccatacctg aaagtgagtc cttctggtgt
    21001 tgtgaatggt cccgcgctga tcgttttcag cggcaagaaa gtgcttattt atgaccgctc
    21061 actctagaca atatttatta ataatccaat tgttgaactg agagaatatg cataaatcgg
    21121 caattttttt atgtcgatgg gatgagcctg attcgtttgt aaaatcaggc aaatttttgc
    21181 aaaggcggat ggtcagaatt taaagcaaac cacagcgacg tttactgcgt tcttccgctt
    21241 cctgataaag ctggaactca tccaaaaccg ggcagcccaa cgcctgctca aatgcttcgc
    21301 gactggcatt accgtgactg cgtgcctgcg tttcattacc cagatttgac tggaagatcc
    21361 ccgccgcgct aacaggcaag aaatcttcat aggtaatggg ttgcgccact acccaaccac
    21421 gttcaattaa gggctgtgga tcgtctccgg gatgaatcgc ctgacgatgc gtctcacctg
    21481 aaggcgtcag acggtaccgg aaccatgcca acccttgctg acgcattaaa aactcactgt
    21541 caggaaaagt gcggaaggtt tcctgtaaat gcatttggtg agtgagatta tcctgcccgg
    21601 ttccagcgtt ccgcagtaga tcatcataca gttgtcgtcc tttcggcgtt aatgccacgc
    21661 cacgctgttc tatttcacca aagcgcgcgg tatgcgtgcc ctgtttctgc cctgcaaaca
    21721 acaccgtctc ttccagtgct ttaaagctag tctggcgtag taaaatcggt acctcgcggc
    21781 gcggcggccc ttcgatcaga attttgggtt caattccgca ttcaggcatc atcgactgca
    21841 cccggtcaat atccagcgtg cgtggcgtca ggtggttgat atggcatcca ggaaaacaga
    21901 ccacatcagc aatcaaccga tgttcgttgt gcaaggcgcg ataggtttct tcatctaccg
    21961 ttgctgactg gtgccaacga aacgtttcca gggcttcctg cacaaactcc tgtgcctgtg
    22021 tttcgttaaa accgccccgc tgctcatatt cctctaacag ttgtcgacaa cgtggggtga
    22081 agatatcgcg ctgacgcaga atctccgccg ctttctggcg caaaatttcg ttctcgataa
    22141 gctcaaggcg gagtaatgaa gtaaaaacgc ggaagggatt acgcgccaga gaagcatcat
    22201 caatgggccg aaatgctgtg gaatgtaccg gcacccccgc ctgcgagaga tcgtaatagc
    22261 tcaccgggta catccccata atggcaaaca tccgccgaag agtagcaagc tcttgtgcag
    22321 tcccaacacg aatcgcccca tgacgttcaa cattcagtcg cgccagctcg tctgcattta
    22381 ccattttttc gtgcagttga ggattgtttt ccagcacagc cagattcaca tcagctacca
    22441 gttccagcag cgtaccgtac tgcggaactt cttgctggta catggctgac attgcctgcg
    22501 aaaactgttc ccgaatctca tccgccgtga tgctgttcgc cataatgtct gcctccagtg
    22561 gtcagtaatc tggagtgtag gtaaccgcat tcactctggc gggaagaatt tgcaaactgt
    22621 gatctcgccg cgaaaacatc aatattatcc atttcgctgt aacataattg ctttaattgt
    22681 taataatatt ttgcaatcaa gttatcataa tcaaacaact tcacttgtca gcgacaccgc
    22741 ttcgttttta acatcgctta tggaaaaaaa tagtctgttt agtcagcgca tccgtttgcg
    22801 ccaccttcat acattcgtag ctgtcgcaca acaaggaact ttggggcgcg cggctgaaac
    22861 ccttaatttg agtcaacctg cgctctctaa gacattgaat gaactggagc agctgacggg
    22921 cgctcgcttg tttgagcgtg gtcgtcaggg ggcgcaactt accttacccg gcgaacaatt
    22981 tttaacgcat gcagtcagag tgcttgacgc catcaacact gctggacagt cgcttcatcg
    23041 taaagaaggt cttaataatg atgtcgtcag ggttggtgca ctaccgactg cggcactggg
    23101 gatattacct tcggttatag gtcagtttca tcagcaacaa aaagagacga ccttgcaagt
    23161 tgcgacaatg agtaacccta tgattcttgc gggtttaaaa accggggaaa tcgatatcgg
    23221 cattggtcgg atgtcagatc ctgaactgat gaccggactt aattacgaac tgctgtttct
    23281 tgaatcgctg aagctggttg tccgccctaa tcatccgcta cttcaggaga acgtaacgct
    23341 aagccgggtg ctggaatggc cggtcgttgt atcaccagaa ggcactgcgc cacgccagca
    23401 ttcagatgca ttagtgcaga gccagggatg taaaattcct tcgggttgta tcgaaacgct
    23461 gtctgcttcg ctatctcgtc aacttacggt tgaatacgat tacgtgtggt ttgtcccttc
    23521 tggcgctgta aaagacgacc tgcgtcatgc cacgctggtg gccctgcctg ttccgggtca
    23581 tggtgcaggc gaaccgattg gaatactgac ccgcgtagat gcgacgttct cttctggttg
    23641 ccagttgatg attaacgcta ttcgaaaatc aatgccgttc tgaaaggtga agggatctgt
    23701 cgatccctcc tttaacattt tcacactgta gcgaaactaa ctggttcacc agctccgcga
    23761 ggttctgccg acacagaatg tttgttcaga cggaatacgt ccaccgcctc agttaatcgt
    23821 gccgcctgtt cttcaaggga cactgccgct gctgaggcct cttctaccag agaagcattc
    23881 tgttgcgtca ccttatccat ttcagatatc gcctggctaa cctgcgttat acccctactt
    23941 tgctcatccg aggcggcggc aatttcctgc atgatatgtg tgacactcgc gacggcatca
    24001 acaatagtgc tcatggtttt cccggccgtg gccacctcat ccgatcccag gtcaattaac
    24061 ctgactgatt cactgatcaa gccttcaatc tctttcgccg cctgagcgct gcggcttgcg
    24121 agtgtccgta cttcgctggc gacaacggca aatccacgcc cttgctcacc cgctcgcgcg
    24181 gcttcaacgg cagcattcag tgccagaata ttcgtctgga aagcaatact gttgatgacg
    24241 gcggtgatct cagaaatttt cttcgaactc gtggagatag cgcccatcgt ttttactaca
    24301 ccggaaaccg tctgcccgcc atcgctggct ttaatagaag cctcttgcgc cagtttgctg
    24361 gcatgatgcg cgttatccgc attctgtttc accgtcgcag tgagttgctc catgctggcg
    24421 gcagtttgtt cgattgccgc cgcttgttct tcggtgcgag atgaaaggtc cgcattgcca
    24481 gcggaaattt cgctggtgcc acgataaatc tcttccgcac cctgtcgaac agtccctact
    24541 gtcatcccca gtgaatgctg catttgctgt aaatgacgac ttaagcgacc gatttcatta
    24601 cgacccgccg gttcatcatt catcgtcaga tcgccactgg cgattttttc aatccgttgt
    24661 gcggcatgtt gcagtggacg aatgacgatc cgacgtagca ccataaatgt tatcagcgtc
    24721 atgaccaggg caagcacaaa cgcgccaatc atgaacatcc cacccaggcg ggtgcgctga
    24781 tgggccagtt ccgccagttg gttggctctg gtgctacgta tcttaacggc tttgctcaaa
    24841 atatcggtat aagcattatc cagcggtcgg atctgctcac tttcatgatt gataatcgct
    24901 tcaaacatgc catttttggc atatttcaac ataggttgca tacccgtgat ataagcctgg
    24961 aagcgttgat ttaattcagt gtcgagggct tcatcagcag gtgttttcac cgaccgattc
    25021 tgataagcac gataaccttg ctgcgactgt ttaatctccg attcggcttg cgcaatattt
    25081 cgcttcattg cttccatttc cgcaatacga ctcgctgccc cggcctgaat catattgatc
    25141 cgggctgaac gcaaaaaatc tgaactgttc gctaacccgg tccgaatctc aatctccgca
    25201 gtaacatcac gctgatcgcg atcggcctgc cagaggaaat agccagccag agcagaactt
    25261 aatgcgaata gaactaagat accgccaaga atgcaggcaa ataacggaac cagcctgatg
    25321 tgatgcaaaa aacctaatcg ctgtgaggga gttgtattca ttaccgttgt ctctcgtcca
    25381 ggtttactcc tgatagaaag tcatcggcac ttgacggtaa ttacttatga ccaaaaacct
    25441 ctttgcgtca catttttaca acaattatgg cagagaagaa taatgccatt ggaatgaaga
    25501 gagtaacgaa tgattaaact ggctaacccg aatgacgaaa aattctggaa aacagcacgc
    25561 aatttcgtca ggtgattatt ttcgtcccta aggaaaccac ataggatgct cacgggtgcc
    25621 tgagactttc tgttttcagg ctaaccggga atcagaaaga cgaaggcccc gaccgatatt
    25681 tctatcaacc gaggccaacg aatgaacaca gcaattcaga tcttagcctc ttacccgcca
    25741 tccggcaagg agaaaggcta tgaagcgcaa ccctctggtg gtgtgtctgc tcattatctg
    25801 cattacgatt ctgacattca cactcctgac ccgacaaacg ctctacgaac tgcggttccg
    25861 ggacggtgat aaggaggttg ctgcgctcat ggcctgcacg tccaggtaag ggcaagcgcg
    25921 gggattttcc ccgcgcatct gactagcaga ttcattttct catggcaccc cttttacact
    25981 tcagttaatg catgtttttt tcgatgcact aatagaacca tcattacaaa caaatttgcc
    26041 gcctgccgta cagtgtgaga tccccccttt cgaaccagaa cagggtttgc gcccggcatc
    26101 ggcataaaag gaaacaagcg ttcctataac caagataagt gccagttttt tcatataacc
    26161 tccatggttt atcgaatctt atgttcgatt ttaacgttat acgctggttt attttgatac
    26221 cgttgatgag atcaaagatc gggtgaattg attaaaaata agccagatgg acgtatgggc
    26281 aaggattatg gttttatttg tcatacaaat aaatataata ggcgcttccg atgtagaccc
    26341 gtattcttcg cctgtaccac gggtcggttt tagtacaggc gttttcttta aatatccttc
    26401 aggaacgttt acgcggcatc atgcgtagaa gtgtgttgtc cttccagaaa tagtggtgtg
    26461 ccagtgcagc cgcagcgtgc aacccgatga caaaatatcc cagattcgcc agcatttcat
    26521 gccacgactt taagctatct acccgttcga aattggcctc tgaagcgtaa ggcatcgtca
    26581 aaccaaacgc aaaccacggg ttgccccggt tatacatcat caccaaacca atcactggca
    26641 gcgcaataaa caggagataa atcaccaaat ggcccaaatg cgccagtccc gtcatcatcg
    26701 gttttggctt aggtataatc ggcggggttg ggtatttcag tcttaacaaa agacgaacca
    26761 ccatcagtac gagaattgag atgccacagg aaacatgaat catgttgatg aggggccgat
    26821 cgctacgtgg gaagaaacca cgaaactcca ttgcgcaata cgctacgata accagcaaaa
    26881 agaccagcca gtgaatgctg atttgtaacc ttgagtattt atttcccata acatttcctg
    26941 ctttaacata atttgccgtt aacataacgg gcttttctta aaatttcatt aaatattgtt
    27001 cacccgtttt cagtacgtta cgtttcactg caagaatgta gattgcccat cccgccatcc
    27061 tggtctaagc ctggaaagga tcaattttca tccgaacgtt cctgacagga gaagaccatg
    27121 agtaaagttg gtattaatgg ttttggtcgt atcggtcgac tggtgttgcg tcgattactt
    27181 gaagtcaaaa gcaacataga cgttgtcgct attaatgatc tcacttcccc aaaaattctc
    27241 gcctacctgc tgaaacatga ttcaaactac ggaccgttcc cctggagcgt tgattttacg
    27301 gaagattcac ttatcgttga tggaaaaagc atcgcggttt acgccgaaaa agaggctaaa
    27361 aatattccgt ggaaagcgaa aggggcagaa attattgtcg aatgtactgg cttttatacc
    27421 tccgccgaga aatcgcaggc gcatcttgat gctggtgcga agaaggtgtt gatttccgcc
    27481 cctgccggtg aaatgaaaac tatcgtttat aacgtcaatg acgacactct ggatggcaac
    27541 gacaccattg tttccgtggc gtcatgcacc actaactgcc ttgcgccgat ggccaaagcc
    27601 ttgcatgaca gtttcgggat agaagtcggc acgatgacga ccattcatgc ctataccggc
    27661 acccagtcac tggtggatgg tccgcgtggg aaggatttac gagcttcacg cgcagcggca
    27721 gaaaatatca ttccccacac tacaggtgcg gcaaaagcca ttggtctggt gatcccggaa
    27781 ctgagcggca aactgaaagg tcatgcgcaa cgcgtgccgg tgaaaacagg ttcggtcact
    27841 gagctggtgt ccattctcgc aaaaaaagtg actgccgaag aggtgaataa cgcacttaaa
    27901 caagcaacca ccaataacga gtcatttggt tataccgatg aagaaatagt ctcttccgat
    27961 atcattggca gccatttcgg gtcggtgttt gatgccacgc aaacggaaat taccgccgtg
    28021 ggcgatttac aactggtgaa aacggtcgcc tggtacgata acgaatatgg cttcgtcaca
    28081 caactcattc gcaccctcga aaaattcgct aaactctgac gcacacaggc ggaggaaaaa
    28141 cctccgcctc ttccactcat taagactgta aataaaccac ctgggtctgc agatattcat
    28201 gcaagccatg tttgccatct gcgccgccaa taccagattt acgccatccg gcgtggaatc
    28261 cttgcatagc ttcgaagttt tcacggttga tgtaagtttc accaaacttc agccctttaa
    28321 tggctttcat cgcgacgttc agattttggg tatagattga tgaggtcagg ccgtaatcac
    28381 tatcattcgc cattgagata gcctcttcca gcgtgtcaaa tgcgaccacc ggcagaaccg
    28441 ggccaaaggt ttcctcatgc ataatcgaca tttcctggcg aacatccagc agcaatgtcg
    28501 gcggataata atatcctttc ccctctaccg ctttgccacc caacgccact ctcgcccctt
    28561 cttctactgc gcgcgccact ttttgctcga ccctttccag cgccgcggcg ttaatcaacg
    28621 gccccatcgc aatgtcgttg cgttcagcgg ggttaccaaa ttgaaccgcc tgcatcgctt
    28681 cacccagccg attgacaaac tgatcataaa tgcctttctg tacataaaca cgttctgcac
    28741 agttacacac ttgcccacta ttaatgacgc gtgaatcaac gatggctttg actgccagtt
    28801 caagatcggc atcgtccatt acgatagccg gtgctttacc ccccaattcc aggcacactt
    28861 tggtgatgtt tttcgccgca gtcgccataa tcttttcacc tgcagagacg ctgcctgtca
    28921 tactgaccat tgcgaccttt gggttacccg ccagttcttg cccaacggtt tcaccacgcc
    28981 ccagaacaag gttaaacacg ccgcgcggaa ggcctatttc atcgacaatt ttggcgaatg
    29041 caatcgcatt gtttggcgta aattcactgg gtttaatgac gatggtatta ccggtcaaaa
    29101 gagcgggagc cattttgcgg gcaatgagga agaacgggaa gttccacggc agaatgccgg
    29161 tagtcacgcc aagcgcacgt ttaaacagaa gaatattttc tcctggacga tcgctttgaa
    29221 taatctcgcc ttcataacgc cgtgcccact ccgccatgta atcgatatag tccgcagtaa
    29281 aagccacttc gacttcagcc agctgctgga tcttaccccc ttcttcaaca atcagcgtac
    29341 tgatttcact ggcgcgttcg cggatcccgg cggagatttt gcgcaaccaa ctggcgcgtt
    29401 caatagcagg caacgcttcc cattctggtt gtgcacgttc tgctgcatcg attgccttac
    29461 gggcatcctc ggcctgacca tcgggaatgc gggaaatgac agcctctgta gcagggttta
    29521 ccacatcaat ccatgcgtct ccacgccagg taacaaactg tccatcaata tacataggat
    29581 gttgaacggg tactgacatg agcgactcct gtgatttata tgtttgtttt tggtaaatgt
    29641 attgttaatt aacatcaatc atcatagaga cggcatcatg aaaggacaat tctgtgaagg
    29701 gcttcataaa aacaagtata aaaggaataa gaggcagaaa taaaaggcag gagttatgcc
    29761 cgtttgcaaa caagtggttc acaaacgggc aatttttaac gtaacgagcg actttccatc
    29821 gcctcgatta gcaccgcatc gtgtttcagc gtttgccatg catggatgac ttctgccgga
    29881 aaatcaacgt gaacgataaa atctcgcccg cggggaccgt agccatcgct atcatcgcgt
    29941 aaacgcggca gctcgccagt gagtagtgag agataacgct caaccggcca taatccttgc
    30001 aacggattaa caaagattac actgtctgcg ttatttccta actgaggaac gaatccggga
    30061 taacttaacc agcgtggtgc atcgggattg tccccagtca tacggcggaa cgtcgccatc
    30121 gtgcgccgct gcatggtggg gtcctgaaca acgatagccg tatgaactcg ttctacggcc
    30181 tgattcaata gcgcgatgct aaagcgtgcg ttttcaccgc agtttgttga ctggtcttca
    30241 atccagattt tttcatgcgg aatgtgccag aactgatgag cgatatccgc caggatggtc
    30301 gcttctgctc tgccagtggt gcggatagtg ttgtagtgcg gatgctgtgc gatggcgcta
    30361 tacaaaaaag ttgtcgagtg accgatacca ccactaatca gtagaggaat ttgctgatcg
    30421 cgagcaatct tacatgccgc gtcgatagtc ggcataaccg cattgcctgc aaggatcacg
    30481 caatcggcct gatacggcat ctcaccggag aaatcatcct gcgccagcca ctgaccgata
    30541 acatttatgg catctatggt tgccggcgaa agcgtcggaa acggggtaat gttcatggtt
    30601 cctccttatc gttcccttaa gaatactgcg tgagataata gtgagaagcg gagcactctg
    30661 aaaggaggaa gtcgtatttc cttaaccgcc actccgcaag cctgacatat cttttaagga
    30721 aatgctgtag ataaacatta ttcatcttgc agctgagaat tgtatttttc tgaattcctg
    30781 aatttttgcc cttaccgttt tagaattcat aaaaaacccg gcgctaaatg cttaccgggt
    30841 tttagattta tcaggcaaat agccgcgtta accgctaatc tgttccatcg cctgcaaaat
    30901 acgcttatct gaaatcggat aaggcgtacc aagttgttga gcgaagtaac taacgcgcaa
    30961 ctcttctatc atccaacgga tctctttcac gtcttcatcc tcacgacgtg cgggcggcag
    31021 tttgttgatc cattgctgcc acgcctgctg gacattttcg actttcagca tctgagcacg
    31081 gtcacgatgc ggatcaaccg ccagtttttc cagtcgtttt tcaatcgcct gcaaataacg
    31141 cagcgtgtcg cccagccgtt taaaaccgtt accagtgaca aaaccgcgat acaccagccc
    31201 gcccatctgc gctttgatgt cagaaagccc cagcgccata gtcatatcca cccgcccttt
    31261 caggcgtttg ttgatattga acactgccgt aaggatttgc tcgacctgct tcgcaatatc
    31321 caccaccgta tcgttcagtt cagcgcggac tttctcatgc agcgcagcaa agccttcttc
    31381 cgtccagacc gggcctccat tggcgtcgat cagttgatcc accccgcagg agatacagtc
    31441 gtcgatcagc tccagcactt tgccatacgg gttaaagtac agccccagct tggctttgtt
    31501 cggtaacttt tcatgcaaat acttgatcgg cgatggaata ttcagcagca gtaaacgacg
    31561 aagaccgttc cacatcgctt gcttttgttc cagcgggtta tcaaacagtt tgatcgccac
    31621 gctgtcgcgc tcatccacca acgctggcca cgccttcact ttgtagttgc cacgcttctg
    31681 ttcgtagctt tccggcagtt gcccaaagct ccagatatgt aagccgctct gctcgatacc
    31741 gtcatccgcc accgcagaca gcgtttcctg cactttgcct ttcagagcat ctttcagatc
    31801 ctgtaatgag cgcccttctt ttagcttctt gtttttgtca tccaccacac ggaaggtgat
    31861 tttcaggtga tcgggcacct gatcccagtg ccagtcttcg cggtcaacgg tgacgccagt
    31921 catccgccgt agctctcgtt caagactgtc gagcaacgac aattccaacg gtgtgacgcg
    31981 gcctagaaac gcttcggcgt agtttggcgc gggcacaaaa ttacggcgta ctggtttcgg
    32041 caacgattta atcagagcaa tcaccagttc gcggcgcaga ccggggattt gccattcaaa
    32101 cccgctctct tctacctgat taagcagcgg cagcggaata tgcacggtca caccgtccgc
    32161 gtccgcgcct ggctcaaact gatagctcaa acgcagcttg agattgccct gatgccagaa
    32221 gttcggataa tccagcttgc tgattttttc cgccccttct ttgatcaaca tgctcttttc
    32281 aaagttgagc aaatcaggcg tttcgcggct gacttttttc caccagctat cgaagtgacg
    32341 agcggagatt acatcgtggc tgatgcgctg gtcgtagaac tcaaacagcg tttcgtcatc
    32401 aaccagaata tcgcggcgac gtgatttgtg ttccagctct tccacttcgg cccgcagttt
    32461 caggttttca cggaagaatg cgtgacgcgt ctgccagtca ccttccacca gagcgtggcg
    32521 aataaagagt tcacgacata acgccggatc gatctggctg tagttgacct tacgcgcagc
    32581 aacaatcggc aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc cctgcgcccg
    32641 ttcccagtgc ggttcgctgt aggtgcgttt aatcaaatgc tgagcaacgg gttctaccca
    32701 ttcagggtcg atacgcgcag caatgcgccc ccacaggcgg ctggtttcta ccagttccgc
    32761 caccattacc catttcggcg gctttttgaa taaaccagaa ccgggaaaga tggaaaaacg
    32821 cgcgttacgt gcgccggtat attcttgttt atcggcatct ttcatgccga tatgggaaag
    32881 caaacccgtc agcaacgcaa tgtgaatttc gcgatactcc gccggttcgc tgttaaccgg
    32941 aatgccaagt tctttcacca cctggcgcaa ctgggtgtag atatcctgcc attcgcgcac
    33001 gcgcagatag ttgagataat cggtacgaca caggcgacgg aaggcgttag aagaaagcgc
    33061 cttttgctgc tcgccgagat aattccacag atttacaaac gcgaggaagt cggattcttt
    33121 gtcgtggaag cggcgatgtt tttcgtctga tgcctgctgt ttatccatcg gacgctcgcg
    33181 cggatcctga atggagagcg cggacgtgat aatcatcgcc tcacgcacgc agccatgttt
    33241 ttgcgcttcc agcaccatac gcgccagacg aggatcgaca ggcaattgcg aaagctggcg
    33301 accgagcggc gtcagtttat aggcgctggc ctgttcatca gtggtgatcg cgcccagctc
    33361 ttcgagcaga cgcacgccat cctggatatt acgtttatcc ggtgcttcga caaatgggaa
    33421 cgcggcgata tcgcccaacc ccagcgcggt catctgcaaa ataaccgagg ccaggttagt
    33481 acgcagaatc tccggatcgg taaactccgg gcgcgagagg aaatcgtctt cggaatagag
    33541 acgaatacag atcccttcgg acacacgacc acagcggcct ttacgctgat tggcagacgc
    33601 ctgagaaatc ggctcaatcg gcaaacgctg cactttagtg cgatagctgt agcggctgat
    33661 acgcgccgta cctgggtcga taacgtattt aatacccggt acggtcagcg acgtttccgc
    33721 gacgttggtc gccagcacaa tgcgccgtcc actgtgcgac tggaacacgc ggttctgttc
    33781 gctgttcgaa agccgcgcat aaagcggcaa gatttcggta tggcgtaagt tcagcttgtt
    33841 cagcgcatcg gcggtatcgc gaatttcccg ctcaccactc ataaagatca gaatgtcgcc
    33901 agggctttcc tggctcagtt cgtctacggc gtcaaaaatc gcctgcagct gatcgcgctc
    33961 ggtgtcatcg gcttcttcaa caatcggtcg atagcgcact tccaccggat aggtccgacc
    34021 ggagacttcg ataatcggcg cattattaaa gtggcgcgaa aagcgttccg ggtcgatagt
    34081 cgcggaagtg ataatgattt ttaggtcagg acgccgcggt agcaactctt tcaaatagcc
    34141 gagcagaaaa tcgatattca ggctacgttc gtgcgcttcg tcaataatga tagtgtcgta
    34201 ctgcatcagc aggcggtctt gctggatctc cgccagcagg ataccgtcgg tcatcagctt
    34261 gaccatcgtg ttatcactaa cgtgatcact gaaacgcact ttgtaaccga tgcaaccgcc
    34321 cggctccgtt ttcagctctt ccgcaatacg gttcgccacc gttcgtgccg ccaggcgacg
    34381 cggttgggta tgaccgatca gccctttaat cccgcgcccc agttccatac agattttcgg
    34441 tagctgagtc gttttaccgg aaccggtttc cccggcgacg atcaccacct ggtgatcacg
    34501 aatcgcttcg agtatgtcct gctttttctg actaaccggc aaattgtccg gataagtgat
    34561 ttccggtcgt gccgcttcac gcagcaggac tttccctgcc gcatggtcaa tctctttcgc
    34621 catctcctgg aaaatggcct gttgtgcatc aggattttta accttcttca cgccgtgcag
    34681 acggcgagaa aaacgcagtc tgtcgcgcag catcagcgaa tccagccgct gctgcaaggc
    34741 cgtaaaggtc aatttttgtt gttctgtcat aacgttagtg ggcagtagtc tgcctgatta
    34801 attttctttt aaatgataga tatagagtac cacatcgcgg ctttctatgc gttgttcaat
    34861 aaattcgaac ataggcttcg atttattgcg ctatctctgt ggcaggattg tgaataaagt
    34921 gtcaacaagc aacggggcat cgcccattca aacatctata aggaaacacc atgagcaagg
    34981 tattagttct taaatccagc atcctggcag ggtactctca gtctaatcag ttgtccgatt
    35041 attttgttga acaatggcgc gaaaagcact ccgctgatga aatcaccgtt cgcgacctgg
    35101 ctgcaaatcc gattccggtg ctggatggcg aactggttgg tgcgctgcgt ccgagcgatg
    35161 caccgctgac tccgcgtcag caggaagctc tggcactttc cgatgagctg attgccgagt
    35221 tgaaagccca cgacgttatc gttattgcgg caccgatgta taacttcaac atctcgactc
    35281 agttgaaaaa ctattttgac ctggttgcac gcgcaggcgt tactttccgc tatacggaga
    35341 acggtccgga aggcctggtc accggtaaaa aagccatcgt tatcaccagc cgcggtggga
    35401 tccataaaga tggcccaacg gatctggtga cgccgtatct gtccacattc ctgggcttta
    35461 tcggcattac cgatgtgaaa tttgtcttcg ccgaagggat cgcatacggt cctgaaatgg
    35521 cagcgaaagc gcagtctgac gcgaaagcag ccatcgacag tattgttgct gaataactgg
    35581 acacgatctt gatgtggact gtagatattc agtccacatc tcaatccgct tacctgtttt
    35641 cccataattt cagcatcgct ttgtgctctt ccttaagcac aatccgcgag cgtctggctc
    35701 gaatataact aatcgcttca tcaacggttt tacaatgtcc gtaacataac agccatgccg
    35761 ccaccaccag cgcactgcgc gataatccca acgcgcaatg gaccagaacg ctgccttgct
    35821 cttcgcgtaa tgtttccagc atcgccacgg cctgtcggag ctccccctct tccggaacca
    35881 ccagatccag catcggtaca caaaaataga gttgatcttt tgtggctcgt ccgcgaggga
    35941 attcaaaggt gacgtccaga acagcattct gtgccggaat atgttgtgga aacgccccta
    36001 aataaacacc agcagtaatt ttgctcaccg actccaggcg aagacaaaac caacgcatag
    36061 acagccacat cccgatgcgc cagggcaatg tcagccagta aacggcgggc gggagtttcc
    36121 cctaactatc tttccctgtt gttatcgcgc caagcccacc gtacccacgg ccaatgatga
    36181 atagcgataa tactggccaa cataaccaga ctgaccacca taactgaagc attatcatta
    36241 gctccatcaa cacaatgcac gagcacgcgc ctacgacata tggcagtgct attttgattc
    36301 gacgttgatc aggtttctga taattccaac gacggtcgac gggcaccatc cagtcaatta
    36361 acatacctac cgccagccct gtgatgacat caataaaatg atgctgccag gtcgtcagcg
    36421 tcgaaatggc gatgagtaaa aaccatccgc cgcagacttt acgccacctc acagccagat
    36481 gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa ggagactggt
    36541 tataaggcag atcaaacaat tcaagttgcg aaaataacca tcctgtcacc ccactcactt
    36601 caggacggat aaaactaaac ttcagcggat agagaaaaaa accgcagcag gccattaccg
    36661 ttgccagaat aagccggtgg accagacggc gctgctcaaa tgtcgagcta caaacaaaca
    36721 gcgaaaatcc atataaaaga tccagactcc agtaaggaac aatagtccag ggaagaaaag
    36781 ggatcgccgt ttcccaaccg aataactgac tggggatatc atggttgtta agatcctgag
    36841 tcgcggtgaa ctgattaaga aatccatatg tgaagaagaa aaacggggcc agcaacaata
    36901 accagccagc gccttgtagc aatacgttcc ggcgttcagt taccacgacg caccgccatc
    36961 gaaaccgtaa aaatgcccca ctcatcaatc cgttgtgtgc atttatcgaa tccggcatca
    37021 tgcacgagcg aatccatctc cccttgagaa cgcacgcgca ttacccacgg tttaccatct
    37081 ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg ctgcccggtg
    37141 tagatgagaa tgccacccgg atcgatggca ttggcaagac ctgcgagtga gttttttacc
    37201 tgctcatttt cgggaaaaag ctcatacagt ccagagacaa tcgccagcgt aggccgcgga
    37261 gttaacgcgc tgagctcctc cgggttaaac gcatcgccct gttcaaaacg cacccgccca
    37321 gacattcccc gttgggcaat catctcttgc ccctgtgcaa catttaactc actgtaatca
    37381 cgtaagaaaa tatcgcttac ggcaggctcg ttcgccagcg catccagtac atagcgtcca
    37441 tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tagcgtggag atcggcaacg
    37501 gcttgtttaa tcagcatttg taaatgggtt ttgcgctggc gaataccgcg ccagccaaca
    37561 ctgttcaggt agattttgtc gactaaacgc ccgaatgcgt tactaccttg cggttgattt
    37621 tgatagacat aatccagcga actgcctgaa tcaaagccgg tgctcattcc gagatgcagg
    37681 cccgcagagt gcgccccgaa caatttcatc gctttacgca taaagcgata cgccaaatca
    37741 accggcgata atggcacggg tccaccagaa agtagccgcc atcgatccgc tgatggtcct
    37801 gtgcagtctt catgctgata atctaatttt tgcgatttgt tagcatataa gtgactaata
    37861 aagctttgca ttttttcaaa taccagcgcc ctgttctctt cacccaacgt gtcgtgataa
    37921 aagcctggca gcagatgcag ctctttcaga gggctacgta aacgctgata aaaatcaatc
    37981 tgcggttggc ggtgcaccac atagtcatcg cctgatatca gaagttgcgt ggggagcgta
    38041 atcgccgccg catcgctaat aatacgttca gacgttttat agagatctag caagatattg
    38101 acggcaatcg cccttgtgat cagcggatca ttattgaaac tagccacccg ttgccgatcg
    38161 tgggtcaaat agcgtccttt cacataggaa ttaataaaaa acagaccacg cagacgatgc
    38221 cataacgcca gcgcaggacg tgccagcggc acatacaatt taaccttaaa ggccggagaa
    38281 gccagcacca gcccgcgaat agcaggcgca taatcatgaa cccatgtagc aaccatcact
    38341 gcgccgacgc tttgcgcgat cacaaccacc tcttccagtc cgacctggct gtcgctggca
    38401 gcaaaacgga caaattcatc gacatcttgc actgaactcg caagagacgg actataacca
    38461 cgcggccccg aagtttgtcc atgccctcgg gcatcccatg cataaaaagc agtatctggc
    38521 atcgccagtt catcaacgag atgttgtaga cgaccagaat gttcatgacc gcgatgaaat
    38581 aagacgatga cctttttcgc ccctggctgt aaagtgggcc agtgccgata aaacaacgct
    38641 gtattatcac tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc cattctctct
    38701 attttccttc agtaacagca atagcgaata aaataaaaaa ataccggagc ggtgaaaatt
    38761 aatgagtcaa tcctgtcaag gagtccgcca tgtcctggta atagttttcc actatcttta
    38821 acaccaacat ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc actaatacca
    38881 attaacaaac ccgctaataa tgcctgtaat gtatctagcg gtgtcagtaa cggtccaata
    38941 atcagtgaca caatcatggt agtgattacg ccccccacca gaccttccag cgtttttccc
    39001 gggctgactt tggggaccac ttttcttctg ccgcaggatt tcccccataa atactgtgca
    39061 atatcgtttg actcggttaa tgccaaaaga aataacacca gtaacgcctc gccctggata
    39121 tttgtggttg gcaacattaa taaccaggcg gcatgactca aggcgaaaac ggtcattatc
    39181 cagccacaaa aaatggcgct cacggtatgc agaaaaccgg agggttcacc cacaaaaact
    39241 cgccaggtgg ctaatatcag aaacccaaac aaaggtataa ataacagaaa cagctcaaag
    39301 caatttaaac cgataagcaa atagttaaga ggaataaccc aatataacca acgcggaaaa
    39361 tgtacggata taagcgtaca atattctttc aatgccagaa aacttatcag cgcaaaaaac
    39421 gtcaacgtca cccagcgtgg acctgaaatc accagtgaaa aacaaatgac gatgccccac
    39481 caggtacgaa tacgtaatgt aacttcatcc ccttttctct ccggtaaccg ccataccaga
    39541 aaacggttta tcagcgttgc taaaattaac aacgcgaaaa gtgttgccag agatttttcc
    39601 agcattattt ttcagcgttc atcaggagca cactccgaca acgattaatc gcagtccaga
    39661 gaagcagaat tgaagcaatg ctccataaaa gattattcca ttgcatccac tgtggataaa
    39721 tggcaacggc cagcacccac aacccaaata ttaacgcgcg atcgcttttg ccaaatggac
    39781 cggcataact gcgaataccg ttaatcgtct gcgcgagtaa accgcaaaac tcagtcaata
    39841 tggtgcaaaa taacatgagt attacgagtg atgcgttact ttccggtaaa aataaaaacg
    39901 gtaaataaag cgcaatatcg gaaataacat cgccagtttc attcaaaatc gccccaagac
    39961 gtgtttgctg attgcactca cgcgccatca tgccatccag cgcattgagt gccatacgga
    40021 caaaaagcac gatgggcaat agcagaaaca tgccatgata tggcaccaac gccaacaaca
    40081 aaccggtgag aagagaaagt gccaacgcgg caagggtgat ctgatttgca gacacttgcc
    40141 gcttatgaag ccaaaacatc gtcggcctta acagtgactg aaagagcggt tttatctggt
    40201 agagcgtcat tatcaatcct tgataattct gtttgcgcta tggtgcatca attgccctgg
    40261 cagagatatc gcaacacgcc gcttcattaa aaattcagaa tatatcaagg tattggggct
    40321 cactgtcagg aatgattatc cccaattaat caaatagtta aaaatattac gctgttattc
    40381 aatgcaactc atcaagtttt ctcatcgctt gttcccgtat tgcgcgcgtc ttaccgttaa
    40441 gcgccagctg gcggactaaa tccagttcag ggttaacaag ctctaacgcc agcaaaggat
    40501 cacgtttcag cctcgccttt aatacatcaa ctggaaaacg gggattattc atcgccacaa
    40561 tccaccactc gggatcaatt tctgccgcca gtactgcagg cggtgtgtgg cggttgttaa
    40621 gtgcgccgaa cttgaaaaat gtgagattgc ttttcgccag tgtactccac catatacgat
    40681 ccggttgttt tgccagtaca tccagttgtg ctaacgcatc gggttctagt gcataataaa
    40741 cacgtaacgc ttcgttaacc tgcgcacggg aactgttttc ctgcctcaga aaaaacttcg
    40801 cccaaatatc atttcgtggc gtgttttgct gtaattcctt taagccttgg gagagttcgc
    40861 attatggcaa gatcaaatgt cgcaatgtct cgtccttcaa atgcagcaac gttgccttca
    40921 tccagcattt gcaattcctg caataattca ggagaagact tagggcgaaa cagcgcttct
    40981 tttttcatct gattacagac attccctgct atgcgaatat tgcttcgctt aaaaatttct
    41041 ttcagagcat cttcatcatt tattaggccc caataatatg catcaggatt tattatcatc
    41101 agcgcttttt tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta
    41161 tttgtcgaag atgcaacaac aggtgcaatt atgtcattga taaatatccc gcgcgcatta
    41221 atacttccct taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt
    41281 gcatttatcg aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgttacg
    41341 ttacgttacg ttacgttacg ttacgttacg ttacgttacg ttaccgagaa taacccatgg
    41401 attagaaaga gagaaagtgg aaatattacc tttgactgtc aaattgccaa gaacaatcaa
    41461 tttttgatta ctctctatat taccttctac cattaaatcg ccacgatgca ctttccagtc
    41521 cgatgcagat aaaccgacgg ggacaagatt cacgctactg ttgatagctt gttcaacttg
    41581 ttgtttatct tcagcggtag ccgcagaaaa aaatggttca atatcaactg catttacaac
    41641 acatcgaagt tccgcctgta ctggaagact cagcataaac aggggaagta aacctatcca
    41701 gctgcaaatc ctgggcttaa acatatgcac tccatgccat taaaaaagaa acaaaaggga
    41761 aatgcatctt tcccttttgt atattcgtta ttctcgcgaa ataccgtcca acgtagacaa
    41821 cacttcctca gaaagatgca atttttcagc agccatattc tcccgtaaat gcgcaacaga
    41881 agacgtccct gggatcagca aaatattcgg tgaacgctgt aacagccacg ccagcgccac
    41941 ctgcattggt gttgcgccca ggctcgctgc tacatccgaa agtgtggagg attgcagcgg
    42001 cgtaaagccc ccaagcggaa agaacggcac gtaggcaatg ccgtcacgag ccaaagcatc
    42061 aatcattgcg tcatcagcac gatgagcaat gttgtattcg ttctgcacac agacgatttc
    42121 ggtaatctta cgcgcctctg caacctgcgt cggtgtaacg ttgctcagac caatatgttt
    42181 taccaggcct tgttgttgca tctctgccag cacggtcagg ctggcttcaa tcgatccttc
    42241 cgctgggcca tgaccatccc ccatcataac gcgcaggtta accacatcca gtgcgcccag
    42301 cccgagatta cgtagattat cgtgcaccgc tttttgcagc tctgccggag aaaatgcggg
    42361 caaccaggat gcgtcctctc cacgccgcgc accaatttta gtgacaattg tcaggtcgtc
    42421 agagtaagga taaagcgctt cgcggataat ctgattggtg acgtgcggac cataaaagtc
    42481 gctggtatca atatgattga cgcccaatgc cagcgcctca cgcagcacgg ttatagcgac
    42541 gtggcgatct cttggggggc caaaaacccc aggacctgcc agttgcatcg cgccataacc
    42601 aagacggtta acggattttg taccgagagt aaatgtattg ctgctcatcg caccctgccc
    42661 tgtaataaaa cggattaaga aaaatagtat agaaacgaaa agagataaat aatgcgaagg
    42721 cgtaaacaag attactggat gagttgctct attgccctga aagacaccaa aacaaaacgc
    42781 gtcaagtacc gcatccggca ataaaaaaac cgcaacctgt atcagcgttg cggtttaggt
    42841 atcacatgct aaaaattaga acgtccagca tcaggacgtt actgtttcgg tcgcagattc
    42901 tcatcaaata ccaggcgctg cctgccaggt tcaacttccc gcaatggctc aacttgatgc
    42961 aacgtctgct tactgcgtgt caccagcacc tgatactcat gcgtgccctg ctttttccat
    43021 gccagttcct gctcactaag ttcacgaatc gcttttgccg ggctgccgac aatcaggtaa
    43081 ttagcaggca tttctgcttt ggctttcaca aatgcggaag cgccaacaat gctgttctcg
    43141 ccaatcaccg caccgtccat cactaccgcg ttcataccca ctaatgcatt gcggtggata
    43201 atgcagccgt gaaggatagc gctatgacca atatgcccat cttcttccac gacagtatcc
    43261 tgatcgggaa aaccgtgcat aacgcaatta tcctgaatgt tcgcgccatc tttcaccacg
    43321 atacgaccga aatcgccacg caggctggta tttggcccaa cgtacacgcc cttgccgaga
    43381 ataacatcgc cgatcaatac cgctgtcgga tggacaaaac tctcttctgg cacaaactga
    43441 gtcagaccgt ctatctgata aattggcata acgcctcctg ttcaatattc aagccgccaa
    43501 aacgttgaaa atacaggctt cccggcgcag gcaattcacc gaccgaggtt tcaccttttt
    43561 cactaacgaa cgctaaagca gcaggcgcta cgcgctgata aagtttgccg ataaaggttt
    43621 ggtagtcgcg caatatatcc gtaaccgtcg tctcgatttt tgtgcagatg cgattcgcca
    43681 tgccgcagat gatgaaaaac tggcaggcat tggctttcat tggggatttt ccgaccagag
    43741 tcatttttca acggtattta agcaacgctt tgggatgacg ccaggcgagt atcgacgtaa
    43801 attccgctaa ctaattcgcg ttgcatgtac agcaacgcga attagttaaa acagatattt
    43861 gactatgcat ttttcagggc gaaaggcaat ttgacaggaa tttttgaata ttcacgttgt
    43921 ttccggaaaa tagattaatc gtcttctcct gtagcgttat cccgatattg cggttttgct
    43981 attaaaattt tcgcacccat ttaccgttta tcccttgtac atattccccg ggtttggccc
    44041 gttctacaag cttttgcccg gcgagtttcg cgatatcatc caccgacaca ttattctgct
    44101 ttgccagttg ttgatagctc gctttacgtt cggcattgat gtcggttact aatttctcag
    44161 tttcagcatc tgttttcagc gcaaccagat aaccataaaa tgtttcgcct acccgcccct
    44221 gagttctggc ttcatccaga gtcaatgcca ttacattgct gcttaccaag ccaacaagaa
    44281 atgcacaaag aagtaatgtt cttttcatca tcacctcaaa aaagatcgct acgggtctca
    44341 agcagctctt cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc
    44401 atattgatag tgatcggttc cttaggtgca gcgacttcaa tgcgaggcgt acagccaacc
    44461 agcataaaag atgacgtcaa cgcagccagt aaaattttca ttgtttatcc tcacactctt
    44521 ttccttgcgg acagtcattt cccggtagat gcgcgttctg ctccagccat gcctggagat
    44581 tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa
    44641 gatttaccgt accgctttta ccatcaacgc gactggtacc tgtaatattg gcctgcatgg
    44701 ttaataaacc gagattatct aaattaattt ttgtcgatga acggctaatt tccatatagc
    44761 gcaaccagtt aattgctgaa cccgcggtca tattgtcttt caccaccgca tctgcggtgt
    44821 ctttatccag ccgcaaggtc attggcccgc tatttgccag ccagccgtct ttcactatcc
    44881 atttttcgtt gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt
    44941 gtttcggatt gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg
    45001 ccgagtcatg ttgcggcatg cgtaattgtt tcatcagcac gttaccgcct aacacatcga
    45061 cgctaacatc agtcaacagc aatggttcgt cttcactcca cggataacgc ccttgcaaat
    45121 cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg
    45181 ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga
    45241 caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaacaca ccgtgccctc
    45301 ccgcgcggaa tccttgttca ggcgcagctg aaaatgcaac ctgagcatac agttcgccat
    45361 cgcgtaagtt catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt
    45421 taggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg
    45481 ggccaatctc accagcatgc aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat
    45541 cgacactaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc
    45601 cggcgtccag tgacagcgcg ccgctaaagg agggatgctg tgcgtcacgt acccagacga
    45661 cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt
    45721 cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca ccctttcctg
    45781 cgacatccca ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc
    45841 agcggccagc gtcagggaga aaatcattcg ccagtccatc catatgcagc acgaaatcgc
    45901 ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaatg tccgtcaaca ccacgttggg
    45961 tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc
    46021 ttcccttcga acgtaataac gcgcctggct caaaggccag cgttgggtca gtcagacttc
    46081 cgcttagctg cgcaggtaaa cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg
    46141 tcagctgcaa aggcagctgg ctgttatcca tacttaattt tcctgggcca aaattaagca
    46201 ccgcgttgcc cttacccgct tgcccctggg tcagcacact cagtcgtccg ctgaccagag
    46261 cgttctcgag ccctgcctgc cagttgtcga ctttgacacc cagtcgtcca ctcaaaggaa
    46321 aacctgcata cggccagctc cagcgaccat cgcttacggt caattgttgg cgagtaattt
    46381 gccacggcag atcgagcaac ggatcgccgt tatcccgtgc caccacaatc aattgcccgc
    46441 tattttcctg ccagtccagc tcggcatcca ccagtgaagg ctcctgcggc aagtttaacg
    46501 tcgcagtagc atgaccactt acaggaagtc catccggcac tagcggcata gtaaattccc
    46561 ccaccagttt aaccggcggc tgattttcaa acgcgacgac atccagttcg ctgactgtaa
    46621 gttgttgccc tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct
    46681 ggatatcgga ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa
    46741 tcagtttatc gatattgatc caggtgttag gcaacatggc ctgccactgc gcgagggttt
    46801 ttggagcggc aggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt
    46861 ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat
    46921 gcgcaagctg acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg
    46981 taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca
    47041 gcgtgggaac ccacagcccg agcgtcatca gcagtgtcaa cggcacaaga ataatcagta
    47101 ataatagcgc gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag
    47161 cgaaaaatta agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg
    47221 cgcctacact aagcatagtt gttgatgaat ttttcaatat cgccatagct ttcaattata
    47281 tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca ctggagaaag
    47341 tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt
    47401 gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc
    47461 taaaaccgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc
    47521 ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt
    47581 caataacgtc gaccttgacg cggcaaaaga actggggctg aaagtagtcc gtgttccagc
    47641 ctatgatcca gaagccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg
    47701 tattcaccgc gcgtatcagc gtacccgtga cgccaacttc tctctggaag gtctgaccgg
    47761 ctttactatg tatggcaaaa cggcaggcgt tatcggtacc ggcaaaatcg gtgtggcgat
    47821 gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc
    47881 ggcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga
    47941 cgttatctct ctgcactgcc cgctgacacc ggaaaactat catctgttga acgaagctgc
    48001 cttcgaacag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga
    48061 ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt
    48121 gtatgagaac gaacgcgatc tgttctttga agataaatcc aacgacgtaa ttcaggatga
    48181 cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt accgggcatc aggcattcct
    48241 gacagcagaa gcgctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga
    48301 aaaaggcgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg cattccaggg
    48361 gagctgattc agataatccc caatgacctt tcatcctcta ttcttaaaat agtcctgagt
    48421 cagaaactgt aattgagaac cacaatgaag aaagtagccg cgctcgttgc gctaagcctg
    48481 ctgatggcgg gatgtgtaag taatgacaaa attgctgtaa ccccagaaca gctacagcat
    48541 catcgctttg tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca
    48601 gaaatcagct ttggtgaaaa gatgatgatt tccggcagca tgtgtaaccg ctttagcggt
    48661 gaaggcaaac tgtctaatgg tgaactgacc gccaaagggc tggcaatgac ccgtatgatg
    48721 tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt
    48781 gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact
    48841 tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt
    48901 tcgctacagc gtttgccgtt gggtaatgca cacatcccaa tcgtcgtacc atccagttga
    48961 cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc
    49021 gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta
    49081 cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc
    49141 tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag ggcggcgtcg
    49201 agagctgtcc tgagcgttgc cccgcatttt tactttttta tggctatttt tttgccctct
    49261 gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa
    49321 agccatttgc taaaattgaa ggattactac tgggcgcgca gcaatttcgt gcgcccctca
    49381 tttgcgcaat gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg
    49441 tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg
    49501 cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac
    49561 gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga
    49621 cgggtgccct ctcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacgc
    49681 tgtacaaact ggcaggcgaa ctgacgccat ttgtcctgca tgtagcggca cgtaccgttg
    49741 ccacacatgc actctctatt tttggcgatc attccgacgt tatggcggtg cgccagacgg
    49801 gttgcgcgat gttgtgtgcg gctaacgtcc aggaagcgca agactttgct ctcatttcgc
    49861 acatcgcaac gctgaagagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt
    49921 cccacgaaat caataaaatt gtcccgctgg ccgatgacac tattcttgat ctcatgccgc
    49981 aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgcg
    50041 gtacgtccgc caatcctgac acttatttcc agtctcgcga agccaccaac ccatggtaca
    50101 acgcagtcta tgaccatgtt gaacaggcga tgaatgattt cgctgccgcg acaggtcgcc
    50161 agtatcagcc gtttgagtat tacgggcatc cacaagcgga acgggtgatt atcctgatgg
    50221 gctctgccat tggcacctgt gaagaagtgg ttgatgaatt gctgacccgt ggcgaaaaag
    50281 tcggcgtgct gaaagttcgc ctgtaccgcc ccttctccgc caaacactta ctgcaagcgt
    50341 tgccggactc tgttcggaca gtggcggtgc tggatcgcac caaagagcca ggtgcgcaag
    50401 cggaacccct gtacctggat gtgatgaccg cactggcaga agcctttaat aatggcgagc
    50461 gtgaaaccct gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc
    50521 cggactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta
    50581 cggtaggtat ttacgatgat gtgaccaatc tgtcactgcc gttgccggaa aacaccctgc
    50641 caaactcggc gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtttccg
    50701 cgaccaaaaa caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt
    50761 ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc gtgagcgagc
    50821 agccgattcg ttccgcttat ctgatttccc aggctgattt tgttggctgc caccagttgc
    50881 agtttatcga taaatatcag atggctgaac gtttaaaacc tggcggcatt ttcctgctca
    50941 acacgccgta cagcgtagat gaagtatggt cgcgcttgcc gcaggaagtt caggccgtgt
    51001 taaaccagaa aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg
    51061 gtctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc
    51121 tgcctggcga tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca
    51181 aaggtcagga tctggtggag cgcaactggc aggctctggc gctggcgcgt gaatccgtag
    51241 aagaagttcc gttgcagccg gttaatccgc acagcgccaa tcgaccgcct gtggtttccg
    51301 atgccgctcc tgatttcgtg aaaaccgtta ccgcggcgat gctcgccggg cttggtgatg
    51361 ccctccccgt ttcggcgctg ccgccagacg gcacctggcc gatgggcacc acgcgctggg
    51421 aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta
    51481 accactgcgt cgctgcttgc ccacattcgg ctattcgcgc aaaagtggta ccgcctgaag
    51541 cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg
    51601 ggcagaaata tgtcttgcag gtggcaccgg aagattgcac cggctgtaac ctgtgcgtcg
    51661 aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc
    51721 gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctcccagaaa
    51781 tcgaccgtag caaactggaa cgtattgata ttcgtacatc acagctgatt acaccgctgt
    51841 ttgaatattc aggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc
    51901 agctctatgg cgaccgaatg ttgatcgcga acgccaccgg ctgttcttca atttatggcg
    51961 gtaacctgcc ctctacaccg tatactaccg atgccaacgg tcgtggaccg gcatgggcga
    52021 actcgctgtt tgaagataac gccgaatttg gccttggttt ccgcctgacg gtcgatcaac
    52081 accgtgtccg cgtgttgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac
    52141 taactgcgtt gaaatcagac gccacgccag aggttcgtcg tgaacaggtt gcagctttac
    52201 gccagcaact caaagatgtt gccgaagcac atgaactgct acgtgacgca gatgcactgg
    52261 tagaaaaatc aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg
    52321 gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat
    52381 gctattccaa caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaagt
    52441 tcggcgagca cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg
    52501 gtcatgttta tgtggcgcag atttctctcg gcgcgcagct caaccagacg gtgaaagcga
    52561 ttcaggaagc ggaagcgtat ccggggccat cgctgatcat cgcttatagc ccctgtgaag
    52621 agcatggtta cgatctggca ctcagtcacg accagatgcg ccaactcact gccaccggct
    52681 tctggccgct atatcgcttc gatccacgtc gagccgatga aggcaaactg ccgctggcat
    52741 tggattcacg cccgccgtca gaagcactgg aagaaacgtt acttcacgag cagcgtttcc
    52801 gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg
    52861 atttgcaaaa acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca
    52921 ccgattaaat gcactggata aggattatcc aattctaaaa aaaagcccgg acgactgttc
    52981 gggcttgtct ttttatatgt tggaaaatca gtagcaatgc aatggcccaa cagaaaataa
    53041 aatctgtgac aataaaggca tataacccgc gcagaataac gtatagcaaa ttaaaagta
//
LOCUS       DAZGTJ010000025        52285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000025 DAZGTJ010000000
VERSION     DAZGTJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.025"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1023
                     /locus_tag="ACHK8E_001853"
     CDS             <1..1023
                     /locus_tag="ACHK8E_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344130.1"
                     /translation="FISSFILTLIYSGYENKLKVYSSITHRISTVCYQNSLFIYCLLI
                     IAYYIYYMTKWNGYIITAFSIISIFIHSYVTCAIRLFRKQKSRLNNTVRYEKLSRKKG
                     FNFWLSLQLIIPGVVQILPMMFLFNQLNFSEGTSDWYEMSILIAITVVIVTIGILPGI
                     IHINERQNGNKMTGIIVVLIFIPVATAALSVWYRPIPNMIANMTMNLSGISDQRTHEY
                     YIERATHPAGMFNGKIWNTRYYKNIPDRFFITGVNTFTLGNIKLICPTAIVKARIESL
                     KFTVNDIDEYEQKGKKLKKTAMKCIPFDKNDIHTWDSPLSEPIYYEKIKQTIDNSMLK
                     ILHVVK"
     gene            1091..1399
                     /locus_tag="ACHK8E_001854"
     CDS             1091..1399
                     /locus_tag="ACHK8E_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP0344131.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            1392..1625
                     /locus_tag="ACHK8E_001855"
     CDS             1392..1625
                     /locus_tag="ACHK8E_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP0344132.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            1635..1952
                     /locus_tag="ACHK8E_001856"
     CDS             1635..1952
                     /locus_tag="ACHK8E_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0344133.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            1954..2307
                     /locus_tag="ACHK8E_001857"
     CDS             1954..2307
                     /locus_tag="ACHK8E_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP0344134.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALSGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            2304..2963
                     /locus_tag="ACHK8E_001858"
     CDS             2304..2963
                     /locus_tag="ACHK8E_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP0344135.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            2960..3727
                     /locus_tag="ACHK8E_001859"
     CDS             2960..3727
                     /locus_tag="ACHK8E_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP0344136.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPATRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPVTATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            3724..5019
                     /locus_tag="ACHK8E_001860"
     CDS             3724..5019
                     /locus_tag="ACHK8E_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP0344137.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            5016..5375
                     /locus_tag="ACHK8E_001861"
     CDS             5016..5375
                     /locus_tag="ACHK8E_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP0344138.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            5372..8014
                     /locus_tag="ACHK8E_001862"
     CDS             5372..8014
                     /locus_tag="ACHK8E_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP0344139.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            8063..8983
                     /locus_tag="ACHK8E_001863"
     CDS             8063..8983
                     /locus_tag="ACHK8E_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344140.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSFPQAVRIQMIIFGFCWRTQTYKIIKLNNSIEH
                     PANLQIEDIQNLSTGRPVLLGDNLHELNEFIENTRQQFTPNTINWWRTPFIALNNWIN
                     QETVNTIGGYIQMVTAHPLGTRISFLTNLNENIIRTSYAGINTTESFGPTIGGFILMP
                     MDGMSLPDENGWDVGNQVTRTAADRVATNR"
     gene            complement(8986..9210)
                     /locus_tag="ACHK8E_001864"
     CDS             complement(8986..9210)
                     /locus_tag="ACHK8E_001864"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0344141.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
     gene            complement(9250..9513)
                     /locus_tag="ACHK8E_001865"
     CDS             complement(9250..9513)
                     /locus_tag="ACHK8E_001865"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0344142.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            9621..9896
                     /locus_tag="ACHK8E_001866"
     CDS             9621..9896
                     /locus_tag="ACHK8E_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0344143.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            10115..10489
                     /locus_tag="ACHK8E_001867"
     CDS             10115..10489
                     /locus_tag="ACHK8E_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0344144.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            10533..11891
                     /locus_tag="ACHK8E_001868"
     CDS             10533..11891
                     /locus_tag="ACHK8E_001868"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0344145.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            11869..12660
                     /locus_tag="ACHK8E_001869"
     CDS             11869..12660
                     /locus_tag="ACHK8E_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFP0344146.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(12763..13182)
                     /locus_tag="ACHK8E_001870"
     CDS             complement(12763..13182)
                     /locus_tag="ACHK8E_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344147.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDPTYQSRKEFLKASTEVELRGQIYLKQHSNEK
                     LKDELANLRSKCAEFVGGKVTKPELSFISAIILLIEV"
     gene            complement(13421..14311)
                     /locus_tag="ACHK8E_001871"
     CDS             complement(13421..14311)
                     /locus_tag="ACHK8E_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFP0344148.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDEI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            complement(14313..15200)
                     /locus_tag="ACHK8E_001872"
     CDS             complement(14313..15200)
                     /locus_tag="ACHK8E_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0344149.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVKQIFGYLLKDNDFVTLLHS
                     NNIHDIPSLLDGSDVSGEK"
     gene            complement(15200..16735)
                     /locus_tag="ACHK8E_001873"
     CDS             complement(15200..16735)
                     /locus_tag="ACHK8E_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0344150.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            complement(16759..17706)
                     /locus_tag="ACHK8E_001874"
     CDS             complement(16759..17706)
                     /locus_tag="ACHK8E_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0344151.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            complement(17725..18729)
                     /locus_tag="ACHK8E_001875"
     CDS             complement(17725..18729)
                     /locus_tag="ACHK8E_001875"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0344152.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            complement(18726..21242)
                     /locus_tag="ACHK8E_001876"
     CDS             complement(18726..21242)
                     /locus_tag="ACHK8E_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0344153.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            complement(21254..21934)
                     /locus_tag="ACHK8E_001877"
     CDS             complement(21254..21934)
                     /locus_tag="ACHK8E_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0344154.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            complement(22048..22560)
                     /locus_tag="ACHK8E_001878"
     CDS             complement(22048..22560)
                     /locus_tag="ACHK8E_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0344155.1"
                     /translation="MKKTMLSACFLLVAGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            complement(22928..23518)
                     /locus_tag="ACHK8E_001879"
     CDS             complement(22928..23518)
                     /locus_tag="ACHK8E_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFP0344156.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            23731..24654
                     /locus_tag="ACHK8E_001880"
     CDS             23731..24654
                     /locus_tag="ACHK8E_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP0344157.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            24666..26027
                     /locus_tag="ACHK8E_001881"
     CDS             24666..26027
                     /locus_tag="ACHK8E_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0344158.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            26024..27526
                     /locus_tag="ACHK8E_001882"
     CDS             26024..27526
                     /locus_tag="ACHK8E_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0344159.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(27671..27871)
                     /gene="glgS"
                     /locus_tag="ACHK8E_001883"
     CDS             complement(27671..27871)
                     /gene="glgS"
                     /locus_tag="ACHK8E_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0344160.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            28205..28495
                     /locus_tag="ACHK8E_001884"
     CDS             28205..28495
                     /locus_tag="ACHK8E_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344161.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            28561..29466
                     /locus_tag="ACHK8E_001885"
     CDS             28561..29466
                     /locus_tag="ACHK8E_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFP0344162.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            29532..29903
                     /locus_tag="ACHK8E_001886"
     CDS             29532..29903
                     /locus_tag="ACHK8E_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344163.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            30077..31666
                     /gene="mobH"
                     /locus_tag="ACHK8E_001887"
     CDS             30077..31666
                     /gene="mobH"
                     /locus_tag="ACHK8E_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFP0344164.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQPYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(31722..32375)
                     /locus_tag="ACHK8E_001888"
     CDS             complement(31722..32375)
                     /locus_tag="ACHK8E_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0344165.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(32487..32753)
                     /locus_tag="ACHK8E_001889"
     CDS             complement(32487..32753)
                     /locus_tag="ACHK8E_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344166.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(32880..33221)
                     /locus_tag="ACHK8E_001890"
     CDS             complement(32880..33221)
                     /locus_tag="ACHK8E_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0344167.1"
                     /translation="MNVTSPKMLSQAIRDARKQRNLTQRETAASMGMKQSTVSEFENH
                     PEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGDLWKYEQKGHGAIRTEPPF
                     SLAYHLDFQST"
     gene            complement(33632..34120)
                     /locus_tag="ACHK8E_001891"
     CDS             complement(33632..34120)
                     /locus_tag="ACHK8E_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0344168.1"
                     /translation="MAKKTAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMS
                     PMTLRSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSR
                     PAAISHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRK
                     GS"
     gene            complement(34428..35237)
                     /locus_tag="ACHK8E_001892"
     CDS             complement(34428..35237)
                     /locus_tag="ACHK8E_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP0344169.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(35191..36189)
                     /locus_tag="ACHK8E_001893"
     CDS             complement(35191..36189)
                     /locus_tag="ACHK8E_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFP0344170.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(36817..38064)
                     /locus_tag="ACHK8E_001894"
                     /pseudo
     CDS             complement(36817..38064)
                     /locus_tag="ACHK8E_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(38287..38371)
                     /locus_tag="ACHK8E_001895"
     tRNA            complement(38287..38371)
                     /locus_tag="ACHK8E_001895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38335..38337),aa:Leu,seq:caa)
     gene            38567..39586
                     /gene="ahr"
                     /locus_tag="ACHK8E_001896"
     CDS             38567..39586
                     /gene="ahr"
                     /locus_tag="ACHK8E_001896"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP0344171.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            39716..41218
                     /gene="yjgR"
                     /locus_tag="ACHK8E_001897"
     CDS             39716..41218
                     /gene="yjgR"
                     /locus_tag="ACHK8E_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP0344172.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(41337..42419)
                     /gene="lptG"
                     /locus_tag="ACHK8E_001898"
     CDS             complement(41337..42419)
                     /gene="lptG"
                     /locus_tag="ACHK8E_001898"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0344173.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(42419..43519)
                     /gene="lptF"
                     /locus_tag="ACHK8E_001899"
     CDS             complement(42419..43519)
                     /gene="lptF"
                     /locus_tag="ACHK8E_001899"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0344174.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(43582..43632)
                     /gene="ytgA"
                     /locus_tag="ACHK8E_001900"
     CDS             complement(43582..43632)
                     /gene="ytgA"
                     /locus_tag="ACHK8E_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP0344175.1"
                     /translation="MSIIILATISTTQENK"
     gene            43786..45297
                     /gene="pepA"
                     /locus_tag="ACHK8E_001901"
     CDS             43786..45297
                     /gene="pepA"
                     /locus_tag="ACHK8E_001901"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP0344176.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            45431..45874
                     /gene="holC"
                     /locus_tag="ACHK8E_001902"
     CDS             45431..45874
                     /gene="holC"
                     /locus_tag="ACHK8E_001902"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP0344177.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            45874..48729
                     /gene="valS"
                     /locus_tag="ACHK8E_001903"
     CDS             45874..48729
                     /gene="valS"
                     /locus_tag="ACHK8E_001903"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP0344178.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(48783..49979)
                     /gene="yjgN"
                     /locus_tag="ACHK8E_001904"
     CDS             complement(48783..49979)
                     /gene="yjgN"
                     /locus_tag="ACHK8E_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP0344179.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVFLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLPANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            50172..50675
                     /gene="yjgM"
                     /locus_tag="ACHK8E_001905"
     CDS             50172..50675
                     /gene="yjgM"
                     /locus_tag="ACHK8E_001905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0344180.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(50721..51137)
                     /gene="rraB"
                     /locus_tag="ACHK8E_001906"
     CDS             complement(50721..51137)
                     /gene="rraB"
                     /locus_tag="ACHK8E_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP0344181.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            51299..>52285
                     /gene="argF"
                     /locus_tag="ACHK8E_001907"
     CDS             51299..>52285
                     /gene="argF"
                     /locus_tag="ACHK8E_001907"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP0344182.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMV"
BASE COUNT        12478 a        14198 c        13480 g        12129 t
ORIGIN      
        1 ttcatctcat cttttatttt aacacttatt tactctggat atgaaaacaa gttaaaggta
       61 tattctagca tcacccatcg gatttctact gtttgctatc agaatagctt atttatatac
      121 tgcctgctaa tcattgctta ttacatttat tacatgacaa aatggaatgg ctacataatt
      181 acagccttta gtattatttc catatttatt cacagctatg ttacatgtgc gattcggcta
      241 tttagaaaac aaaaatctcg cttaaataat acagtgcgat acgaaaaact atccagaaag
      301 aaaggattca acttctggtt atccttacaa cttataatac ctggagtagt acaaatatta
      361 ccaatgatgt ttctgtttaa ccaacttaat ttcagtgagg gtactagtga ctggtatgag
      421 atgagcatac tcattgccat aactgtagtt attgttacaa ttggcattct gcctggtatc
      481 atacatatta atgaacggca aaacggcaat aaaatgacag gaattattgt tgttttaata
      541 tttatacctg tcgcaaccgc tgcacttagt gtttggtaca gacctattcc caacatgata
      601 gctaatatga caatgaatct ttcagggatt agtgaccaga gaacccatga atactatatt
      661 gaaagagcaa ctcacccagc aggtatgttt aatggtaaaa tatggaatac tcgatactat
      721 aaaaacatcc ctgatagatt cttcattact ggagttaaca catttacttt agggaatata
      781 aaactcatct gcccaacagc aatagtcaaa gcaagaatag aaagtttgaa attcactgta
      841 aatgacattg acgagtacga acaaaaaggg aaaaaactaa aaaagacagc gatgaaatgt
      901 attccctttg ataaaaatga tatacatacc tgggactcgc cattatctga accaatttat
      961 tacgagaaaa tcaaacagac cattgacaac tctatgctaa aaattcttca tgtagtgaaa
     1021 tgatttttta gctgcttcac ctctgaagct ctcctacttt gctaatcatt gagtataagg
     1081 agagcttcaa atgcagtccc agacagcact attactgtta ataatacctt tcccggtact
     1141 ggtaatcgcc tccccggaat ccacccagct cgccctcgcg ctgaagcaac tcgacggcgt
     1201 taaaacctcg ctggagcacg cccggaccag cgccagcaac gatccgcagg cgcgctactt
     1261 cttcgactac ttacaggcgc agcaggacat ctctgtcgtt gaacaaggca ttcgccgcta
     1321 tcttgccccg gctagagcac agccgcgtgc tattcagccc ctgagcgcca actaccagca
     1381 ggagaatccc gatgactgac tcgcaactcg ccgcctttca ggccgccagc ctgagcaaac
     1441 cggagaccgt cagcctgctg gtgctcggcc tgtttaccag cgtcctgctg ctgtggtgtg
     1501 cctggacgct aatggtcgcc tggcagggcg tctgccgcca gcgtctgagc tggcagagct
     1561 ttggcgcggt ggcggcccat tcactgttac tggtgctagt cagcttctgg ctggtactga
     1621 gctgaggagg catcatgcgc tggtttctgt tactgctgtt cgccacattt aacgccctcg
     1681 ccgacctgcc gacgatggaa gatccgtcac gcggtcaggg caagggcatt atggagacgc
     1741 tgcaaaacta cggttttgac atcgtggtgc tgctggcgct ggccatctgt gccgtcggtt
     1801 ttctggtagt ggcgaacaac tgtattgcta cctattcgga gatccagagc ggacgcaagc
     1861 agtggaaaga tctgggctcg atggcaggcg tcggcagcat tcttctggtg gtcatcatct
     1921 ggctgctcaa ccaggcttcg gacgtcctgt gatatggcaa ccatcccctt tctccccgac
     1981 cgcctcaacc gcgagcccgt ggtctggcgc ggcctcacca ccagcgaact gtttctggcg
     2041 ctggctctcg gtcttggcgg cggctgtgcg ttcggcattc tgctggccct gataacccac
     2101 tactggccgt tgatccccgg ttcggcactt tctggcgcgg cattactgat tcagggtggt
     2161 gggcggattc tggcccgcgc aaaacgcggt aagcccgaca cctggctgcc gcaggcgctc
     2221 ctcgcctggc tggagcgtca tcatcttcgc ggcgcacaac tgatccagca cagcgccgtg
     2281 tgggtggtgc gcaggagtcc acgatgagcc gctttcgcca tgcgctggct gagcgcgata
     2341 accatatcct gacgctgcgg gcggtgattg ttctgctgtg cctgtgtctg gcgggcgtcg
     2401 gctacggctg gtacagcgcg ccgcaaaagc tcaccctctg gttcccgccg gatctgcgca
     2461 gcggcagcac ccgcccgtgg tgggaagtgc ctcccgccag cgtctacgcc ttcacctttt
     2521 atatcttcca gcagcttaac cgctggccgg ataacggcga ggaggactat tcccgtaatc
     2581 ttcgttcgct gcgtgcctac ctgacgcccg cctgttacac ccggctggag cacgattttc
     2641 aggtgcgtcg caacggcggt gaactgcgcg accgcacgcg cggcgtctat gaactgcccg
     2701 gccacggcta cagcagcctg gtgcatgagg gcgagaagcg ccccttcccg cgcgtggagg
     2761 cgctgagccg ggattcatgg cgggtaacgc tggatgtggc ggtggaagag tactaccaga
     2821 acgagccggt gaaaaaagcg ctggtgcgct acccgttgca ggtggtgcgc tacgccatcg
     2881 atgcggagaa aaatccgtgg ggactggcga tcgactgtac cgatgccgcg ccgcagcgtc
     2941 tggtggcgga ggagaaacca tgaagccagt cctctttact ttattgttca gtttccccgc
     3001 cttcgccacg gaagtggtgc agtgggaaaa catcccgctg cccgtcacgc tgcatgtcgg
     3061 gcaggagcga attgttgacg tgggtaaacc ggtgcgcatt ggctatcccg ccacgctgga
     3121 aggcaaagtg cgtctgcaaa gcgcaggcgg caaggtcttc ctgctggcga acacggcgtt
     3181 cccggccacc cgcatccagt tacgtgatac cggcaacggt gagctgatcc tgctggatat
     3241 tcatgccacc cagggcactt cgacacttga accaatgcaa attagctata cgccgcagac
     3301 gcctgccaca gcaaaaacat ccgtcccggt gacagcatca acagaacctc tgcccgtctt
     3361 gctggtgcgc tacgccgcgc aaaatctgta tgcgccgctg cgcaccgtcg aagccctgcc
     3421 cggcgtcaca cctgcgcccg tccggctggc gaaacgcatc accactttac tgccgcagca
     3481 gccggttacc gccaccccgc tggcgtcatg gcaggtcaac gccaccaccg tcaccgccat
     3541 ccgcctgcaa aatcacagcg gacagcgcat cacgctcgat ccgcgcgagc ttcaggggca
     3601 gttcaccgct gccgcgttcc agcatgactg gctcggcccg cgtggtctgg cagaagatac
     3661 caccgtggcg tacctggtga cggaaggtcc ggtttcccgc gccctgctgc cggagcccaa
     3721 accatgatgc cgcgccagag cctgctgctg aaaatcctgc tgccgctcat cgtactggta
     3781 acggtatggc tgctgctgcg cccggccagc aaaaacacgc aggagacttc cctgtcccgc
     3841 accgtggcgg ccttaacgcc gcaggaactg cgcgatctgg gcatcgacgg cgacacgccc
     3901 gccgacaccg tcgccacgct ggtcggccag atgaaacagt accgccgcga acttcaggca
     3961 atccagacaa acaacgaaag ccagcaggcg cgcgaaaaac ggcttaacgc acagcaggcc
     4021 acgctggcct ctcagcttcg ggaagacatg cgccgccagc aggaccagat gcgcgatgaa
     4081 ctggtgaaaa gccagcgcgg gctgctggat aagctcgacc gtctgatggc caagcgcccg
     4141 gacgatatcc cgcccggtct ggggctgacg cccggcgaac tacctgccga cgttcaaccc
     4201 gatatccgct ggctggagcc gctggatgcg cctccacctg tggatgaaaa gctgctgcgc
     4261 gtccctctga aagcggccga caaaccaccg ccgaaacctg acgtgaaggt ctacaccctg
     4321 ccgcagaacg ccacgctgac cggatcggtg gcgatgacgg cgctgattgg ccgcgtgccg
     4381 cttaacggca ccgtcagcga tccctacccg tttaaacttc tgattggcga ggacaacctc
     4441 accgccaatg gcgttgagct gccggacgtg gcgggcgcgg tggtctccgg catcgcctcg
     4501 ggagactgga cgctctcctg tgtgcgcggg caaatcacct cgatgacctt tgtgtttcac
     4561 gacggcatca tccgcaccgt cccgcagggc ggcaaatccg gcaacaaaca gcaggagatc
     4621 ggctggcttt cggataacca ggggctgcca tgtattcccg gcgagcgtaa aagcaatgcg
     4681 aaggagtacc tgacctcgca gttcctgctg gcgggttccg gtgcggcggc gcaggccttc
     4741 gccaatggcg aaagcaccac agtggtcgag gacggcagca tcaccagcgc agtgacgggc
     4801 aacaacggtc agtacgtgct cggccaggct attggcggcg ggctgaaaga gaccgccgac
     4861 tggatgaaac agcactacgg ccagatgttc gacgccgtgt acgtgccgcc gggccacaag
     4921 gtggcggtgc atctcaccga agcgctgtct atcgactacg agccggaagg ccgtcaggtg
     4981 aaataccagc aggccagcac accgggaggt ctggaatgaa atttaccatc cttatacttc
     5041 tgttgttact ggccggatgc agcacctcgc aggaggaact gatgcctgtc gaccccaatg
     5101 ccagcatgct gacgctgtgg cagcagcaaa gcggcggcaa cgcggctctg ctggatgccc
     5161 gcgcccagct acgccgcccg ccagatggtc cggtggcgca gcctgaggca gacactatcc
     5221 gcagccagtt tccgcgcctg cccaacccgg atatggtgct ttacgtcttc cagcacctca
     5281 acagcaacac gccggtaccc ggctacagca ccgttttccc cttctatacc cgtccgcagt
     5341 atgcgttgcc gggcgaacgc ctgacgccgc catgaatgaa cggcgcggcc tgactgaaca
     5401 ggcgctgaat accagctacc gcaagggccc gtcatttgtc gactggctgc cgtgggtgga
     5461 gtttttgccg gagtcgcaga cgctgctgct ggaagatggc cgctcggtag gtgccgtatt
     5521 tgagctatat cccgttggca ccgaaggccg ctcaacggcg tttctggaac aggtccgcga
     5581 taccgtcacc gatgccttac aggatgcgtt tgatgagcat gagcgcgcac cgtgggtggt
     5641 acagtttttc tgtcaggatg aggacgacag cgaggcgtgg ctggcatcac ttgcctctta
     5701 cgccgcaccg gaaatccgcg actccgcctt tacccgcgag tggctcacac agatggcaaa
     5761 ccatatggtg aatctcaccc gcgacgaggg actgtttctc gataacaccg tcacccgtac
     5821 ccgctggcgg gcgacgtggc ggcgcacgcg gatggtggtg taccgatggc ttcctggact
     5881 ttcaccggat gaaccgtcgc cggaagagac gctgaataat atctgcgaac gccttaccgc
     5941 cgggctaaaa gcggcgggta tcagcgctat ccgccagaat gagcaggaca ttcaccgctg
     6001 gctggtgcgc tggtttaatc ccgcgccgcc tgctgaggac aaaaaagcct tctggcagca
     6061 gcttgacctg ccggaaactg aaccgctgcc ggtcctggcc gatttcgccg aacgcctgct
     6121 ggcgagtgaa ccccgttcgg atgcggcaaa cggcgtctgg tggctggata accgcccgca
     6181 taaagtggtg gtggttgacc gcctgcgcaa agcgccgccg cccggcctgc tgaccgggga
     6241 aaccgcgcgc ggagacggca ttaacgccct ggtcgacctg ctgcctggcg gaaccatcat
     6301 gaacctgacc gtggtggtgt atccgcagga caagctggag caacacatca gccgcctggc
     6361 cgaccgggcg attggcgaca ataccgattc ggaatacacc cgccgcgact gcagcgaggt
     6421 gcgaagcttc ctgaaggacg gccataaact ctaccgcagc acgctggcgt tttatctcag
     6481 cgcgccggac accgatatcc tgttccgcca gcagcgtcag ctacagagcc tgctgctcaa
     6541 cgccgggctg gttccggtgc aggagcagca cgaagtcgcc cccttaagcc actggctgcg
     6601 ctggctgccg atgtgcttcg atcccctgcg cgacccgcgc gggctgtata cccggctcaa
     6661 ctttgtgcag catatcgccg ggctgctgcc gctgtacgga cgggaaaccg gcaccgggca
     6721 tccggggatc agctacttca accggggcgg cacgccgctg tgcttcgatc cgctgaacaa
     6781 gctggatcgt gcccagaacg gacacctttt acttcttggg ccaaccggag cgggcaaatc
     6841 cgccaccctc aacgcgaaaa tcgcgcaact gatggcgcta catcgtccgc gcctgtttat
     6901 catcgaggcc ggaaactcat ttggcctgat ggccgactac gccgccgccc acggcctgac
     6961 ggtgaataaa atcagcctgc gccccggttc tggcgtcacc ctgcccctgt tcgccgatgc
     7021 gtggcgactg gtggaacagg cagttaccga cgccgaaccg ccagaggacg aagagagcga
     7081 cggcgatgac cagcgcgacg tgctgggtga aatggagatc accgcccggt tgatgattac
     7141 cggtggtgaa gcgaaagagg atgcgcgcct cacccggccg gatcgggcga tgatccgcca
     7201 ggcgattatc gctgccgcgc acacctgcta cgaggaaaaa cgacagacgc tgacccagga
     7261 tatccgtgac gcactgttcg ccctttcccg cgacaacagt ctgccggaag cccgccgcca
     7321 gagggcgtat gaaatggcgg aatccatcaa cctgttctgc gccgggtttg aaggcgaact
     7381 gtttaaccgt gaaggccaga tgtggccgga ggcggatatc accctagtgg atctcgccac
     7441 ctttgcccgc gaaggctatg aagcgcagct tgccatcagc tatatctcgc tgattaacca
     7501 tatcaataac ctcggcgagc gcgcccagca tctggcgagg cctatcgtca atatcaccga
     7561 tgaggcgcat atcatcactg ttaatccctt attggcgcgc tttctcacca aaggctcgaa
     7621 gatgtggcgc aagctcggta tctggctgtg gctcgccacc cagaacctgg cggattttcc
     7681 tgatgaggcg aagaagctgc tcaatatgat cgaatggtgg gaactactgg tgatgccgcc
     7741 ggaagagatc gagcaggtct gccggtttaa agcgcttacg gaagaacaga gccaactgct
     7801 gcgctcagcg gtaaaatcac cggggcagta taccgagggc gtggtgcttt caccgcgtat
     7861 agaggcactg tttcgggtgg tgccgccagc gctctatctg gcgctggcga tgacagagaa
     7921 acatgaaaag gccgatagga tgcggattat gaaggagaca gggtgtagtg aagtggaggc
     7981 ggcgataacc ctaagctcgg taatctcgca ataaaaatat gctaagctat tcgcattaat
     8041 gttaaataac tggaaaaacc ttatgaccgc aattgcatgt tggtttaata gagaggaaag
     8101 cgatagtatt tgggcagtca gcgactcaag aatcactcag caaaatagca gactaactga
     8161 ccattgtccg aaattattct caatccccgt atcagttatt cgctcttcgg acgtttatcg
     8221 aattcacccg caaaaaatat tcgaatttgg cttcggattt gcaggaagta caatgattgg
     8281 aattaatgtc aaagaaatgc ttgcagtatc attagcaaga cttcatgaga ttgcgaacaa
     8341 taatccagaa caacaaatat cgaccgaagc attcccaagt cttaatgaaa tagcttcatt
     8401 agcaaaaaat attgccgagc aatatatgtc tgatatcggc caatcctttc cccaagcagt
     8461 aagaatacag atgataattt ttggtttttg ctggagaaca caaacctaca aaattataaa
     8521 actcaataac tctattgagc acccagccaa cctccagatt gaagatattc aaaatttatc
     8581 aacaggcaga cctgttttat taggtgataa cttgcatgaa cttaatgagt ttatagagaa
     8641 taccaggcaa caatttactc ctaacacaat aaattggtgg cgtaccccgt ttattgcttt
     8701 gaataattgg attaaccaag aaacagtcaa tactattggt ggatacatcc aaatggtaac
     8761 cgcgcaccca cttggtactc gaatatcatt tttgaccaat ttgaatgaaa atattatcag
     8821 aacaagctat gcaggaatta acactactga gtcatttgga ccaactattg gtggttttat
     8881 cctaatgcca atggatggga tgtcactccc agatgaaaac ggctgggatg ttggaaatca
     8941 agttacccgt actgctgcgg atagagttgc tactaataga taaacctatc tttcaaagtt
     9001 gaataattct ttgatgtcca tgtccaaccc ttttcctaaa acacttatca cctcaagggt
     9061 tggattcctt actccccgct caataccgct cacataagta cgatcgagac cacacttatc
     9121 cgcgaatgct tcctgagaca ggcccgactg gagtctgagt tttttgagat gtgcgccaaa
     9181 ctgcgcctga atactggatg tatttttcat ccagataaaa tgaacttacg ttgtctatca
     9241 gaccacggac tatgagtcac acttcgttga ttcggcgtcc ttttctgcgc catcgtcaaa
     9301 cataaataaa tggctaagat ccatccccag accgtccgag attgcacaaa tgacaacaag
     9361 cgtggggttc cttactcctc gctcaattcc actcatatag gtgcgatcga taccgcattt
     9421 gtcggctaaa gcttcctggg ataggtccat ctcacggcgt agagccctga ttcttttacc
     9481 cagtaccttt ttgattgatc ccgtcttttc catctagcca gcatcaatct tcgttgatta
     9541 tcagtccacg gactatgagt cacataaaaa gcgtgctagc atacattcgt gactcatagt
     9601 caacaataga ggtaaacgga atgactaaaa aagactggca ccccgccgat atcattgccg
     9661 ccttacacaa gcgcggcacc agtctggcga aactctcgcg ggaagcggga ctggcctcgt
     9721 caacgctggc gaatgccctg aaccgaccgt ggccgaaagg tgagtggctg attgccattg
     9781 cgctgggaaa gcatccgtca gaaatctggc cggaacgtta cctcaatacg ggaaataaac
     9841 gtgaaccccg taaaccgctt cacctcatga caacaacaga aaactccggg acataacgac
     9901 ccgcgctgtg agtggcagga cgcattaatc aacgacacct gacaggagag ttgtttatgc
     9961 acaccgaacc acacccttgc tccatcgctc ttacaaccgg aggccacctt cacccggcct
    10021 cccggactga aggctttatt cacctattca ggctacatgc ctgctcacct acaccaggcc
    10081 gtactctcat gcccgacaaa cgggagaacg agccatgatg acctactgcg cacgcatcgt
    10141 tgctatcggg ctgtttgtcg ccgacggact ggcagacagg acgcttatca cctttgacag
    10201 caacggaccg aaagactgcc tggactattc cctgtcgctg gaaccctcat tccgcgaaga
    10261 cgacctgctg atcctgcccg gcgatcggct gctgctggcg agccatgact acctggtgac
    10321 cgccgtgggt aaagaggcac agcagtcttt gtttgaactt ggccacctga ccatggtgtt
    10381 taacggcagc ctgaatgcct gccattcggg agccgttcat ctctccggcc caatccctaa
    10441 acttcacgac ctgtacggtg aactgctgat tgaggaggga cggccataag cgctgccttg
    10501 aatccatcca caacaacaaa agagaagaca ttatggaact caacagaagt ctgaaagccc
    10561 gtcacctgac gatgatttcc gtgggaggcg tgattggtgc cggatttttc ctcggcgcag
    10621 gcgcggccat tgcccgtacc gggccgtcgg tggtgctggc ctaccttatc ggcggtgtca
    10681 tcaccctgtt cattatgctg ttgcttgccg aaatggcggt cgccaggccc gtctccggtt
    10741 cctttgaatc ctatgcccgc accgcctttg gcccatggtg tggctttatc accgggtgga
    10801 cctactggct ggccttcctg attggcccgg cgtcggaggc catcgctgca ggcaccttcc
    10861 tgaacctgtg gtttcccgga gtccccgtct ggctgttttg cctggtgatc gcttcgtcgc
    10921 tgactgccat caacatggtc ggcgttcatt ttttcggtga agtggagttc tggctgagcc
    10981 tggtgaaagt cgtggcgctg ctggccttta tcgtcgcggg ctgctacagc ctgggactgg
    11041 ataacgcctc acccgccagc atcagtcatc cgaccgacag cggcagcttt tttgctggcg
    11101 ggctgcccgg atttctcggc gcgatgttaa tggtgatttt ctctttcggc gggacagagg
    11161 ccatcggcac cgccgcgggt gaaagcgaga acccacagcg tgatattcct cgtacgctgc
    11221 gtggcaccgt ggtgcggatc ctgttgctgt atgtcggctc attgaccgtt ctgctgctcg
    11281 ttctcccctg gcagcaggca ggtgtcagca gcagcccgta tgtcgaagct tccggcattc
    11341 tcggtggcca tatggcgcgg catatcatga actttgtggt gctgaccgcc gcgctctcct
    11401 gcatcgatac cggggtttac gccacctcgc ggatgctgca cgccatggcg tctgacggct
    11461 atttcccggc ctggtttgcc cgcctgcacc cggcacataa aacaccggat aacgccattc
    11521 ttgccagcag cctggtgctg tttaccggcg tcatcatcgc cattctctcg ccagatgcgt
    11581 atgtcatcct cgccgggatt tccggattcg gctttctgtt cacctggctg atgattgcct
    11641 taagccagcc caccctgcac cgaatagccc tgcacgacgg aacgctgaaa tacaaggctc
    11701 cggccgcccg ctacatccag ccactcaccg tggggctgat tgcgctgatc atggccgggc
    11761 aatttttcac cgacggcggc ggcattaccc tggttaccgt cctgatctgg ctggccctcg
    11821 ccagcgcgta ctacatcttt gtcgtccgaa aaaacaggag gctgtccgat gaaaaaagtc
    11881 atgctcaatg aactgactgg ccgggaaatc cgcgagctgt tgcagtctgg taaggaactc
    11941 actgctatcg tcaacttcgg cagttgtgag aaccacgccg atcacctgcc actcggcccc
    12001 gatatgtttg tacccgaaat cgtcgcctgc cgggtggcgc agcgggtgga taataccctt
    12061 gtgctgccct gcacgccgtt cggcacatcc gttcactaca atgcctatcc actggcggtc
    12121 accctacgct acgagaccaa tatcgcgctg gcggaagata tcttcatcag cctgctgcac
    12181 cacggtattc gccggatcct ggtgattaac ggccatgacg gtaatattcc ggcgattgaa
    12241 atcgccgccc gcaatgtgaa ggcccgacat acgcaggcag cgattgtcgt ggttccggcg
    12301 tggtgggatc tggtgggaca tcatctcggg aagggatttt tcgactcatg ggataacacc
    12361 ggactaggac acggcggcga gggcgaaacg tccgtcatga tggcgctgat tccggagctg
    12421 gtggaactga aatatgcgca gaaacagatc ccggtggagg tgttcgctga ttatggcgtg
    12481 aaccggatct gggatatcgg ggaaatcagc gccaccggag caaccggcga tccgcgctgc
    12541 gccacggcgg aaaaaggcca gcggatcctc gcctgtctgg tggatgtgct ttgcgcgttg
    12601 ctgagaaagc tggatgcagc taactggcaa tatgcgttaa ataaagacgc aaacatgtaa
    12661 tcgatatgct cgggcaacct gtggagtggc ttttgccttt ccacaggtta cccggaagcg
    12721 atatcccaac cgggtaaccg attggtgtta gcccagaatc tattatactt caatgagtaa
    12781 tattattgct gagatgaatg acagttcagg tttagtcacc ttaccaccaa caaattctgc
    12841 acatttactc ctgagatttg ctaattcatc ttttaatttt tcgttgctgt gttgcttaag
    12901 atatatttgt cctcttagct caacttcagt acttgccttc aaaaattcct ttcggctttg
    12961 ataggtggga tcgttatcga gattttttgg tatagttatt aaacatttct gaaatgcgat
    13021 taaagcttcg attaggagaa gtctagagtt ttctttgtat tgaggcttcc actgatgcaa
    13081 cgaccgccaa gcaagtagta aacctatcgt tgtcacaaca aacgtcaaac aggcagcaat
    13141 agcagcaacc cccgcccatt ttgcagcttg ttgattagcc accaatgtgc tcaagcttat
    13201 gaaatccgca tctatatgca ctatttttac ctcagtaaga aaagtggatg taaatctact
    13261 ttttcatttt tgaaacagca acttgatttt aagccatcgt cctttgagca agattttccg
    13321 caccagttga atcacgtcaa ccaccagcac gccctcctct ctcagtgcat cccatcataa
    13381 tggcaatctg taaaacagca gtccttgctt taaaaatgtg tcattcctca attgtctggc
    13441 caagcttatt caaatcatcc agccccgaga tttgcttgag cacagaaagg tactcctgat
    13501 ggttatcata aagccagtta agtacctgac cgttcgccag cagacgttca atgtgatttt
    13561 taatgataac cagccgaagg ttattctcag catattcatc ctgaagatgc tgcgtttcca
    13621 cctgaacgga agccatttct ttttccagtc tttcgagatt ttttctgata tctgtttctc
    13681 tgctggcatc ttttttaggc gctacaagat gttcaggggc aacggcatgc agcatcgaca
    13741 tcgcaaattt gcgggtgaaa ttattaatac tgatcatggt gttaaccatt tctatctgct
    13801 tatagaactt tagttttttc aacacagaga aaatggtctg cggtacattt ttttctgcca
    13861 gtaacgccat gacttcaggg gcaatgccat cagaaatttt cagtttacca atcagcgtat
    13921 ctggcgtgac acccagtacg gcgcatagtt tttcaactga aacgccagat tcaaccgctt
    13981 taagcagcat gttttgggcc tgcacaatgg taaggcgact aacacgtttg ttatatgaat
    14041 atgtatcctc cacaggggat atcagacaat gagcagtagt tattccgagt tctttcaaaa
    14101 cttcaacgcg aagatgtcca tccagaatga ctttatcatt ggcgtcattg atataaacca
    14161 caatgggttc aattaaccca atttcatcga cagaggcttt aatttgtttg tacttcgtac
    14221 tgaatttaac attaaccggc agcgttctgg catacaccag tgattccacc ttaaaggtat
    14281 aaaatcggga gtcaaaacat tgtgaaatca tcttatttct cccccgatac atcactgcca
    14341 tccagtaaag aaggaatatc gtggatatta ttactgtgca gcaatgtaac aaaatcatta
    14401 tctttaagta aataaccaaa tatttgcttt actgccagaa ttttctcttt aaccattgtg
    14461 gatttcagca gaatggcttt ttgctcatta atgttttcct ggtagacatt agccagttct
    14521 tccggggtca gttttttctg ttttttccca ggaaaaaaag ccgtactaat cgtaccttta
    14581 tcgcctttct tacgttgttc aagtatgcgc ctgatcttat taatttgccc acccttgata
    14641 tgtccttctt tcagggcgac cgtcagcagt tcctgagttt cctcatctcc tgaccgggat
    14701 atttccacag caagatacaa gggaatatcg ccagtctcaa atgcccgcaa aagatgcttc
    14761 tctccggatg acaacagagt gatcacccca tttacccaac tcgaattata cccaagccgc
    14821 aagcctattt cacgatcggt caggccactg gtcttcattc ttctgatatc ttccattagc
    14881 tctgccgccc ggggattacg tcttgcgatg ttttcagcca ggctcatcag gtgcgctgtc
    14941 tcttcatcaa cctgcttgat gattgcagga attttcgttt caccatactc aatatacgct
    15001 tcaagtcgtc cctgaccaca aatcagggca tatttatagt tttcattatt cactgggcgc
    15061 acgctaacag gccgtttcag accaatggtt tttatgttct cttttatttc attatgaatg
    15121 aatttattcc ttttccgtgg atttgcgata taaatatcac tgatatcgac aagttctatc
    15181 tgttcatcaa aagatttcat tatttactct ccagaccagt tcgttttaca agattttcca
    15241 gacatgacaa atcatcaaat cgatatagtt cgaggataaa accattgttc tcatgaagcg
    15301 ttaaatcgtt atacaacaca tcaatagccg gaaacataaa gtaatcaacc acggagttat
    15361 ttgtgctatc caaccgtgga aagatcatga catcacaata atgggataca ttaaatttta
    15421 tcttccaacg aagcttacct gacgacaaac gttggcagcg aaccagatta gcggaaacaa
    15481 aaaactcatc attaatatat aatgaaccat cctgatgcat ctctgcccaa ccgccatatc
    15541 ggtatatatc ctgcaccaaa ttatccatga aagtgagatg gcattctgca atctgctttt
    15601 ttatttcaag ataagagtaa tcatggcgcg ggtgataacc aataagggtg taagccctga
    15661 ctaacccacc aaaccgtgaa cgaaaaatgc tgctcgatgg aaacagttcc tcttcatcaa
    15721 tcacaatccc ggataatctg ccacgaagct tcagcagatg gcgtagttta tccagcagtt
    15781 catcatcact gtactgaacg gccctggctt caataatcct tttagccgcc tcaaatttct
    15841 ctactgatac cacagggtcc cacgcgccgt cacatctgat ccactgatcc tgaggattcc
    15901 tgacacttcg ggtcttcaat cgtccggagg ttctgttata aacattattg ccaatatatt
    15961 tttcattggt cagtatctga tgaattttac atcggctcca ctggagccca ttctcggcgg
    16021 ggatgttctt ccggttcagt tctgaagcaa taacaaactc aggcttgccc tcatcaataa
    16081 acatgtcata gattttattg actattacca cctcttccgc gggccccggg atcaatatta
    16141 ctctgtcagt ttgcaggctt ttcctttgcc ccggcagaag catgcctttc ggattagtat
    16201 gttcatcaat taataatctc cgaatgccat agccaggcat accaccttga taataacccc
    16261 gccgaacgag gtttgcctgg ccaagaaaaa ctttttgtga aaggttttta ctgtaagttg
    16321 cggcgccata gcgggagtaa gataatccca gcataaacaa ttcggtctgt tcttccgaga
    16381 aaggctccgc gcaataaata atgcgcacac cattacgctt cagtagccac gagtaatgag
    16441 cagcctcttc aatatcctga aaacgaccaa accgactgac atcgtaaaca agaattaacg
    16501 agacttttat tattccgttt tcaacatcat ataacagcga acgcaggcca ggcctgttat
    16561 ccaggctcag cccacttcgg ccagcgtcgt cataggtata tttaatggta atacagtgtt
    16621 cttttgcata cttctcgata tattcccgct ggttctccgg cgaatatttc tgatgctcgg
    16681 tagacattct taaatattga gcagcaatcc tgttcccggc gacaatatcc gacatagaat
    16741 attccatatg attacgcgct atttataact catcgtaaac gtcgccgtac cgtttgccgc
    16801 acctggcgtg atcgagcctg cagtctggta atatcgcgcc ttaagctgca cgatctcgtc
    16861 accatatacc gactgccagt aaaaccgttc ctgaccgtac tggacagcgg catcatccgg
    16921 gcggtaatga agttgtatcc ccacgccaga cgccccgccg ctatcaatca gcatgacgcc
    16981 ttgcgaactg gcggaagcat ccgcagtggc atcgatacgt acgtttattc tcgccccgac
    17041 atcgcagctc aaaccaatat caaagctcac ccagtcggtt gaagatcccg caccactgaa
    17101 ttcgctttta tcgtgatccc ccatcgggac atcaacagag ggcgtggtca cagtacaagc
    17161 ttctgatttc actgtgttca tcccgttcaa cgtgacgttg gcaaaataaa actgattcac
    17221 taccgaagcg cgcgccagca caccgtccgt taatgtaccg ctggtgatat ccccggtttt
    17281 gataagctcg cccttgatgc catcaccggg aatggatacc caggtgccgc cagcgttgta
    17341 ggcgtagctg gtttcatagg gcaatgtttt acctgtcgag gccgcactca gacgtaatcc
    17401 tacccctggc aggttggtgt tgtagacacc attcccatag ccactcagcg tcccccattg
    17461 cgtcagttcc cagcgggcgg tccaggcctc ccgcttacac ccggcaatgc cgtcgccatt
    17521 gttgtatgcc cctgtaatgg cagtggcaat cgtgctgccg acctgtgcat cccgctggat
    17581 aaccacgttg ccgaaattca ggtaacccga tacttctgat gtcacaccac cgtagaacga
    17641 acaggtggcc tgaacctccg gcaggacggc tatcatcaac agcaggaaga aaagaatttt
    17701 tctcatctca acactccggc gacgtcattt ataggtcagc gtgaaggtcg ccgtggcatt
    17761 ggcaacacct gcagtaatcg tggctgcggt ctggtaatac cgcgccttaa gctgcactgt
    17821 ctcagtaccg ccattgggag aggtatagta atagcgtgac tgaccaaact gtacggcgct
    17881 gttatccggt gcaaagaaca actggacacc gatgcccgta gccgccatgt ctccgggcgc
    17941 gctgtccagc ttcatcacgc cagccacgtt ggacggatcc tgtacggcat ctatctgaac
    18001 gttgatacgg gcacttttat cgcagtccag cccgatgtta aacgcctgcc agccggttcc
    18061 cgttcccggc ccggagaact ccgctttttg atgctgcccc agcggcacat tgacatcagg
    18121 cgtggtcact gagcaggcga tggtggtgat ttgcccaccg gaaaatgtaa tgggaatttc
    18181 agtcgaccac tgcccgttct cccttgtccc cgcgataaaa cgccccaggt tgccgctcag
    18241 cgtaccgggc gtaatgtcgc cggtttttat cagccggatc cgcgcctgat cgttaatgga
    18301 gggatggtag ccaacgctgt ggcttatctg gagcgtgaat acctgcgact gcccgctggc
    18361 gataccgatc cagttttccg gtaacggatt gctgtcgacc gaggagcggc cttcaaacac
    18421 aaatcccaca cctgcgacat tactgttaaa caccgcctca ccattccagg tgacgctggc
    18481 gcgggagccc gtgtttttga tgcctgcatt gatgacgtat tgtggcgttg catcataatt
    18541 gcagtcctga taggccatcc cctggttcga caccagccag tcgctaatgg gttcccccac
    18601 cacagcatcc cgctggacca ccaccgtacc ggggctgatg gagaaactca ccgaagacgt
    18661 gcaaaacgcc cacgacgcct ggcagaacag gatccccgcc agcagaatca tcccgcgcca
    18721 tactctcatt ttgccacctc cctgcattcc tggttcacca ggatcactga cggctggttt
    18781 tcctgttccg gtaaacggta atgcaccacg cactgttgtg ctccgttcca gttaaccgca
    18841 atatcgcctt cctccggcac gccagtcaga tagacctggc catcatcgcc gacaataccg
    18901 ctgccgccct ctttcagctt cgcctctgcg ccaaagggga ctggcagccc acggtacagc
    18961 agcgtcatga gcacacgccg cccgacacgg gtgttaaaat ccgccatcac cacggcaccc
    19021 tgcgtggggg tcacggtctg tacggcagtg tcggtatcca cttcgtcgcc aagcgtgctg
    19081 gtgtcgagcg caatacggtt tttcttgtac gggttcacat acggcactac cgcataaccg
    19141 cgccagtcgg tgtaaacccc ggtgttgttc tgtaccttcg tatcatccgc accgggcgct
    19201 ttcacgatcg ccagcgactg gccgagcggc tgcgaaagcg tgatgccatg cggatgcgcg
    19261 acaataccgc ccttcagccc gtagttaacc tgctgcgtgt cgtcgtcata gttgtagcct
    19321 ccggtgattt ccccctgccc gcctttatag tcggcgttga tattgccgga gccgcccaca
    19381 ccatggttgg tgtagctctg ctggaggctg tagctcaggt tgttgtcggc aagcgccgta
    19441 ccgttcagcc ctacctgctg gcgggtatcg ccgtgtttcg cggtgttgac gctgtagctc
    19501 gcccaggcgt tcggcataaa gcggctgagc ggcacctgga tgttgaacgc cagttgctgg
    19561 tcggcaggct gcgtgctctc cgggtagtcg ctgtaactcc aggtaacgct gtagctgata
    19621 ccgtcgatat tcccgttcca gcccgcgctc aacgtgcgtt cgtagccgtc ctcctgccag
    19681 taatcctgct ggtagccgga gataaaaaac gagccatagc taccgatact ctgactgaga
    19741 tctaactgga ggcgctggcg tttgttgtac gtcatctgcc agccgttatc atcatcagcc
    19801 ctcaggtcgt tggcttcctg gaaggtgtaa aatccggcgg tggagtagcg atatgccgcc
    19861 agcgtgaagc tggtgtcggt ggtctgaaaa tccttcgaat actgcacccg gtacgactgc
    19921 ccgttgtggg tttcatcgtt attgagcgtg gtatgggcgg cggtgatatc ggctgacagc
    19981 gaaccgagtt caccgaggcc aaaaccgagc cctgcggcgc cagactgata atcgtccgag
    20041 tacagcgtgc cgccatagac ggtgatagca tgcggcagac cgtaaatcgc agtgacctgg
    20101 ccgaactgcg gctcgtcgct gtcctcattg ccgctgcggt atttcccggc ggtcagggca
    20161 tatttcaggc ggccttcacg ttgcataatc ggcaccgccg agaacggctg gacgaaggtg
    20221 cgctcgctgc cgtcggcctc tttgatggtc acttccaggt caccgctgcc cgaggtcgga
    20281 tacagatcgg tgatggtaaa cgcacccggc gcgacgtagc tctggtagat gatgtagccg
    20341 ttctgcttaa tggtcacctg agcgttagac tgggcgatac cgcggatcac cggggcgaac
    20401 ccgcgcaggc tgtccggcag catgttgtcg tcagatgcca actgcgcgcc gcgaaactgg
    20461 acgctgtcga agatgtccga cggcgtgtag ctgtcgccga gcgtcagttg tcccttcagg
    20521 cgctggatat cccgttgcag gtacgtgttt actgaatccc agtggctacc attatcttcg
    20581 tcataattcc aggtcgagta gttgcgtagc cgccacgcgc cgaggttgat accgctttgc
    20641 aggttcgcgt agtagttatc atccgaaccg ttttgtccat cgcgccaggt gttgctgccg
    20701 ctgaggttgt agttcagcag tgcggcgggg acaccttcgt cccattcgga cggatccacg
    20761 tatcctctgg cgctggcatt cattgccgcc tggggaatac tgacgttcaa ctgcaattta
    20821 ctgaagcgca gttcagtgtt ggccatcggg atatagcggc caatatcatc cagtggttga
    20881 tccggcggca gtgctgccag tgccgggaag acgtccgttt tgacgcccca ggctttcagc
    20941 atcgcaggcg ttatctgcgg gctgagtttc tttttggctc cgtcaataaa agcaatatcc
    21001 tgtgcgtcgc tctgcttttc accgttcacg aagaccgtta cgcggtaggt accaggtagc
    21061 tgaccaccgg attccgcaaa ggtggataaa tccgccaccg gctggccggg gttatcaatc
    21121 tccagcgcgt gggggttaaa cacctcacgc gcctcggcat tacgcaaata gccagacaaa
    21181 agcactattg ccaacaacag cgtcagcagg attatcatca gacgtggcgt cgggttattc
    21241 atcacgtcct ccttcacagc ttacggcttt cggtctggct gacaccgcca tagtcgctga
    21301 tggcctgcca ggtcaccgcg cttgcagtcg ctgacggtag cgtgaaactc acactgcctt
    21361 tcggcggcac catatccgcc tctttaatct cctgcccgtc gaccttcagg gtgaacagcg
    21421 tgacgtaata cggtgtggga ttgctgacgg taagatggtt accgctgtgg gcaacgctca
    21481 actggtgata ggcactctcc ggctgccctt ccaggcctgc cggacggtaa aacagtttca
    21541 gccgcgactt gaccactacc tgcaacacgt tggtgcgcga atggctgtcg tcggtagcgg
    21601 ggatcgattt cacgttcagc cagaacaccg actccctgtc ctgcggcagg ttgcctccgg
    21661 tgcgcacaat gcgcagcatg ttctcttcgc cgggattaat gcgaaacaac ggtggcgtga
    21721 cgataaacgg cgttttgccc ccctgttcgc cactgtctac ccagctttgc accaggtaga
    21781 cgccggattt ctccgggttg cgtaccgagg cggccgtctc ttttttatcc ccgtcataaa
    21841 tcacgcgcgt accgccaatc acgatcccgg cctgcgcctg tacgctcagg aaagccagca
    21901 acagtgccag caccacgtct gcatacctgt tcatcttctt gttctccgga cgaaaaatac
    21961 gcatccctgc gaaacagtcc ctgttatgcg gcggtacacc aggtcgggta ggatgggaga
    22021 ggtggtgaac cccgcgacgg cacagcgtta tttgtagtta atggtgaact gcgaggtacc
    22081 gttagctgta cctgtggtta ccgcggtacc ggtggaaaca tagcgggcga cgaagtccag
    22141 cgtattgctg cctgccgcga gggggtaatc cattgaggcc gtatgcagag gaatgactgc
    22201 accattttta tcggcgattt cgataccaac gccggtcgca atcccggtac cgctgtccag
    22261 cgccagcagg ttctggttta cgccatcaga cgtcccgtca aacttcacgt tcgccccggt
    22321 aacggtatca gggcaaccac tcaggttgag cgtgaagcgc gtcggggagg ctttcatccc
    22381 ggccgcaccg ttcagtgcgg tttgccctac attgcccaga tcgacgttca ggttttgcga
    22441 accgctgtcc acggtgcatg tctggtcggt aatactgccg gtaaaatgga tggtgccatc
    22501 attggcgacc gcattcccgg ccaccagtaa aaaacacgcg cttaacatcg tttttttcat
    22561 acgtttctcc ttgatgaaag atcactcagg gctcaaccct gattcactct ggtgacgaga
    22621 aaacccgcgt aagactttcc cgttagtgcc agagtggtga gttcaggaga aaatgtggtg
    22681 gacgaagggg gtgaaaaata aaatgtcagt ggaagtgaag tatggctttc aaaatggcat
    22741 aaaaccgtaa tgcggtgagt gagtaaaaac tggcaacgtg aaccacttaa gcacgttgcc
    22801 agccaggacc agagagagac tacatatttg ttacattaaa tattatttta aaaacaacaa
    22861 gatagtccgt taccaaaagg ggtctcagaa aaattttgac agtttgctaa atttttctga
    22921 tactacttta cattctggca ctaattgtgt ggttttctct cacgtttccc cagacattca
    22981 taaacctctg cgtatttgtc gccgtataaa tcaccgtatg ccggatgttc cggtgcccca
    23041 gataatcctg tatcagacgg gtgtcgcgcc ccagatctgc cagcgcgtaa ccgcaaccgt
    23101 gtctcagcat atgcggataa gcggaaatgc tgacacctgc cagttctccg taacgtttga
    23161 gtaacaatct aacctgatgg cgcgacagcg caccaccctt ctgcgacagg aacaaccagt
    23221 ctgaatccgc atcccgccag cggttgcgta ccgccagcca gcgcatcagc gcaggcagtt
    23281 cattatcaaa cagcggatgc tgcgtggaga gactgttttt caaccgccgg acatacaccg
    23341 agcgcccgat cagatcgata tcagacaggc gcatcccgca cagttcactg acccgcaggc
    23401 cgtgtaaaaa acacatctga atgaggcaat aatcgcgctc aaaatggatc cctctgtctg
    23461 ctgcaagcaa tatctgtccc acttcatgac gagtgagatg ctttcgtttt tcaggcatca
    23521 gccttgctcc ttatcaaaat aaaaaatgaa aaaaaatcag gccagaactg ccttcacagt
    23581 ccaggcctga accagagaat accaccagaa acaacgtctg gtgatgatgt aaaactcaac
    23641 aattttctgt aacggcttcc agtttcatac cgttgatgaa cagatattgc ggtacacccg
    23701 acagaagaaa tatctgctgt cggaggcgcg atgcgagccc aatacatcct tctgctgctg
    23761 tgcgctgctt tccccctcag ggcgattact acgccggaga tcgcagcctc tgcgcttacg
    23821 ccggactgtg tgaaataccg ggtggtcggc ctgtgctact ggctgtactg cacgccgttc
    23881 ggctgtacgg tacgcacgtc ggtgaaggtc agccatttcc gcccggatct ggtggtgtcg
    23941 gcttacagca acaccgggca gaatccgtgg gcggagatgt cgttactcag cccaccgttg
    24001 cccggtgttg ccgaaggcgg cggcgatacg aatcctcgtt ctgccagcca gcacagcaag
    24061 gtccgcttta agaatgccga tgccatcggc ttcccggcgg gtgatgaact ggcaaaattt
    24121 ttcacccagt ttggctatat ctgctcacct tcatcgcagc cttttcaacc gtactttctg
    24181 agtcaacccg atacgctggc gtggcgcagc ggtgtgccgg aaatgacata cccggaggcg
    24241 ctgacgccgg gcatgcggga agtcggtcag aacggcgata tgtggggcaa tatctacccg
    24301 cgagccggag ccatcaacca gacgcacgac tataaagccg ccgccgttat cgcccagcgc
    24361 gcggccgatc tggtgacccg taccgggcaa ccgcatctct acacgccgct gaccgcctca
    24421 tcgcgcgccg ggtactggcc gccctccccg gttatcgaag gcgacagcga taaccatcgc
    24481 tggcaaatgc tcacgccgaa aaaatccgcc gcctgttcgg tgttcccgga tggcagtgcc
    24541 actgacacct acgccaacaa actggcggag gatggcgcgt acgcctggac gctgtggcgg
    24601 ccctataaat gctgtccgcg ccgggggcaa acctttctcg gcagtacggg ttaacggagg
    24661 cggtgatgag agcccttttc ctggtcttat tgtttttcgc cggggtttgc gtggcggcag
    24721 acgactacag tttccgcacg cgaggcgcgg ttgacgacct gctttactac cagattggcg
    24781 gcggtgaagc ggtcatgccg cctccggtac gccgtagccc ctcgccgctt agcttaggtc
    24841 ttggctggaa cgccgatatg atgtgcggca atttcgacat cgacaccacc gtgcgcaacc
    24901 agctcaacgg catcaccgac ggcttccagc aactgatggg caacgttatc cagagtgcca
    24961 ccggggcggt cgccagcctg cccgcgatga ttatccagcg cgccaatccg cagctttatg
    25021 atctgctgac caacggcgtg ctgcaggggc ggctggattt tgataagtct ctgctgaact
    25081 gccagaaaat ggcggagaag atgaccgatt tggcggccga ttccgcctgg ttttccggag
    25141 ccaaagcgga gaactaccag tccctcgccg ccagcgataa cgatgccatc cgcaccgacc
    25201 agaaagcggc aaaagaggcc gccgaaaagg gtaaacgctg ggtgggcggt gaaaagcgcg
    25261 gcggcaaagg ccagctgccg attaaggtag tccatgacgc caccgccgca ggatggaata
    25321 tcctcaacca gcagcctgcc accagcacta cttctcttag ctcctctgaa tgcgacgggg
    25381 aactttgtag cacctggaca tcccctgaag aggccgccgg gtggatgacc cgcgtcgttg
    25441 gcgagcagac catcagcgtg gcgcaggtca ccgacgatcc ggatgcgcgt tccggcgcgc
    25501 aggcggggat cggcctgcat ccgctgatcg acgaagaaaa ggcgaagatc ctgcccctcc
    25561 tgacagatct ggttaatggc agtcagaaac ccggcttcga caatttggcg aaagcttccg
    25621 gcggttctat gcaactgacc cgcggcgtgg tcgaggcgct gcgcgacgat ccggacgttg
    25681 ccgtcctcac cgaacggctg gcgggagaac tggcgctggc ccgcgtcacc gagcaggcgt
    25741 tgctggcccg gcgtacgctg ctcgcaggta tgcgtgaacc caatatcgcc aacgtcaaag
    25801 aggcgcagga gagcctgggc aaaaccaccg cccaactgga tgaagagctg aaccagttga
    25861 agctggaact cgatttccgc caggcactga cgcgcaatac cgccagccag atcctgcaac
    25921 gcaaacagca gcgcgaccag cttcagggac aggtggtgga ggtgccggac gacagcgaca
    25981 gccgtctgca caacctcaat aacccgcagc cggacagccc gcgatgaccg ccaacagcta
    26041 tttcgagtac gtgctcaccc tgctcggctg gctggtgaat aacggcgtgt ggcagacgct
    26101 gaccgccacc gggctgttcg ccgtgcccct gctggcaaaa ctggttgccc tgtggctgaa
    26161 agcgcgcggc cagggggccg atgaaggcaa cgtcgcccgc ctgctggtcg tctgggtgga
    26221 gcactcgctc tacagcgcac tgctggtgat cttcttcgcg ggtgtgccgt tcctcaatgt
    26281 cgatatcaac accctcacct acgacagcca gcgcacaaag cagtgcggct ggtcggtggt
    26341 acagcccggc gattccggct acgcaccttt gattaatgaa ctcagcggcc agagcgcttc
    26401 cgttccagtg tggtggtact tcacgcatgc actgggcaaa ggcatcacca gcgccgccgt
    26461 cgccgtattg ccctgcaagc cggacctgcg ccagttgcgc tttgaggtgc agcacacccg
    26521 gctcgccgat ccggcactgg cgcaggagct acaggatttc gttaacgact gctacgggcc
    26581 gtcgcgggca aggctcaagc ggctgagtag cgatattagc gaggagcaaa gccgggaagt
    26641 ggactgggtg ggatcgtctt tttacctcgc cacgctaggc tattacgacc gcgatcactc
    26701 ccgttcgccc cgcgccgcct ggccttatga cgccagccgc gacgacggtc tgagcgatac
    26761 gggtggcggt ggctatcctg cgtgtaagga gtggtggtcc gatggcgata cggggctgaa
    26821 aaaccgcctg ctgaagcagg tcgataccac gctctggcag caactgcaaa aactgggaca
    26881 gtctaaagag gagtatgaag aagcggtact gcgctcgctg gtcagcccgc gcaatatgca
    26941 ggtatcgcag ggcggactgg tgtacaacgg gtttggtggg agtgcggggg ataacctcag
    27001 ccagacgcag ttgctgagtc gtgtgggcgg gatcatcggc gcggggcttt cgagctacgc
    27061 cctgtttccg gcgtttgata acgtccgcca ggcgctgccg atggtgcagg cgttcctcga
    27121 aatggcgctg gtgatttgta ttccagtaat tctgctgttc tcggcatggg atcttaaaac
    27181 ggtcatcacg ctgagctttg tgcagttcgc cctgttcttt ctcaccttct ggtgggaact
    27241 ggcccgctgg ctggataact ggctgatgca gatgatgtac gacagcgata cccactcata
    27301 cttcaacctc tggggcctgc aaaacacctc cgacgatctg attgtaaata tcattatggg
    27361 cgtgatgttc ctggtgttac ccgcattctg gctgggggcg ctgacgtggg cgggcatcag
    27421 gatcggcgca gcagtcgctg gggggatggg cagcgcggtc ggcgatatcc gcagcgctgg
    27481 cgaacaggtc gggaagatga tcgtcagtaa aacacgcatt ccctgaagat ttcgtactga
    27541 cggtgtgatg aacagggctt gtagctgagt ttatgcgcac cgcataaact gtcgtgtcag
    27601 tgacaatctc gtggtaggta gctggagtct gaaaaaacgc aacgggccgt taagcccgtt
    27661 gttcgttaca tcaccgcagt tctatcactc tcgcccgctc agcctgcttt cggtagtggt
    27721 cgtatcgctc ccggaaccat gcttgctgtt catcactcat attgccggta acggcatcga
    27781 tatcaactgg ctggcccagg gcgtgcatac gggcaaaact gcgcgccaga aaatcaaaat
    27841 tctttaatgc gtttatatct tgtcggttca ttagcatatc cctccagtca ttgtgtttac
    27901 tgttcaaggt catatgcttt tctgatttca gtatccgcct gaaaaagatc gttttttgag
    27961 cgatgcgtgc gcatcaatct acctgtgttc acatttcagg aacgacaaaa ctcttcagcc
    28021 ccagtaatga ttttcccctc ccttttatca cccgcgcaga aaacactacc gccagccatg
    28081 cagtaagttc gccatgtatc cggaaggtgc gaacggcgca gatgcgcagg atacccgtac
    28141 cattccatcc cgcagggaaa cgccctgcga agggtgttcc ctgctcattt acaggagaaa
    28201 caccatgtcg cttttcgata aaaaaccgtc gttaatgccg gtggttatcg cccctgatgt
    28261 ctggcaatcc gcagtggaat ttcccgccga cccgcagcag gtggaagaac ggctgggcaa
    28321 tctgttgctc gtcgtactgc tgaccctgcg caccgccggg acatcccgca agaaagtcac
    28381 ctttacgatc tactgccagc cgccgcaggg cgatatgaaa gtccctgtcg cattaccgct
    28441 atcgctcaac tatcacggcc actatgtgca ggtctcgatg gctgaacagg cataacctct
    28501 ccccgccgga gtcgcttccg gatggaggag gctccggctt ctttccatga ggaccacacg
    28561 atggacagaa gcaacgatat ctaccagaaa gtcaccgacc agattatcgc tgagctggaa
    28621 aacggcaatg tgccgtggct ccgcccgtgg cgcgatggcg agccgccgtt cccgatcaat
    28681 gcgttatccg gtcgtccgta tcacggcatc aatgtaccgc tgctgtggaa cagcgccgac
    28741 aggcagagct tcgccagcga ccgctggctg acgttccatc aggtcagcgc gctcggcggg
    28801 caggtgcgcc ggggggagaa atcctctctg gcggtgttgt atctgccgcg cacgcaggaa
    28861 gagaccgacg cactcggcca gccgttgctg gataaagacg gcgagccgaa ggtgcggcat
    28921 ttcgggatta ttcgtgaatt ttggctgttt aacctgtcgc agtgcgacgg cctgccgcag
    28981 gcgctgtccg agccgtttac ccggcccgca acgcctgtgg agatggctga agccattacg
    29041 atgaatagcg gtgtgacgct gcgccatcgc cgccagtcga aagcgtatta caaacccgcg
    29101 accgatctgg tgatgatgcc gcacccgcaa cagtttgaaa gcagcgaggc gtactacgcc
    29161 acgctgctgc atgaactgac gcacgctacc ggacacgctt cacgccttaa ccgccccggt
    29221 attgccggag cggaacgcac cgaagcactc tacgctgctg aagagttagt ggaggagatg
    29281 ggtagcgcct tcctgtgcgc ccattgcggc attcagggca ggctccagca cgccagctat
    29341 atcggctact ggctggatat cctgcgccag gacaagcggg cggtgatccg cgccagcggt
    29401 atggcgcgcc acgccagtga atggctgctc aacgctgtac cacaaccctg tcagcttagc
    29461 gcctgacagc ccacccgcac ggggagcctt ccccgccggg aagttgctcc cctttttctg
    29521 aggatcaatg tatgggactc gatctcttct ttttcgaacg gaaaaaaaac gcgcccgatg
    29581 aaagccagta cgccagagtg ggttatttcc gcaaggtgaa tccgctgctt aaatgggtgg
    29641 ataaacacgt tgggctggtg cgcaaccaag tgctgttaga agtcaccaaa gcaaatttgc
    29701 aggcgcttct gagcgacctg cagagcctga cgccggataa ctgcggcgcg gtttatccga
    29761 cgattgacgg ctttttctat ggttataccg attatggaga acactactgg aaagacgttc
    29821 gcaccgtcac tctctgggtg aaggagacgc tggagacgtt tgattttgag cgagacaggc
    29881 tgatgtttta tgcctggtgg tagtattaaa gtggccgaca aaacgtcggt cactttgcct
    29941 cgtcggccag aaatacagcg ccagatatac acatgagagc ataccgtttt tactaataaa
    30001 aatgtaaaag gcccaaaggg agacactgca aaggaagggc gacaagggca aaggggcctg
    30061 cccggaaagg ggaaatatgc tgaagtggct aaaaagacca tcccagagcc agccacctac
    30121 gccagacgct acgctgccgg agggctggtt tgcgccacaa tccgcagaac aattgctggc
    30181 gacgccgtta cgccagtcct gtttgcacca gttacggcaa ctgaccccgc tgccggatac
    30241 cctgtttgat agctggatcc tcgcggcgat tcatcgcttc gccgcgttgg tacaactgct
    30301 tcccgcctca caaagtcatc accattccac tcccggcggg ttgctggatc attcgctgga
    30361 ggttgcctgc aacgccatcc gactgcgcca gggttatctg ctaccaccgg aagcgggcgc
    30421 ggaagaacag gcccggcagg ccagcgcatg gacggtcgca gtggtctgcg tggcattact
    30481 gcacgatacg ggaaaaatcg ccgtggatat ggagattcat caacgtgatg gcacccgctg
    30541 gcattcgtgg cagggttatc tggcacagcc ttatcgctgg cagtaccaga gccacggtcg
    30601 cgactatcat ctgcatccgg cggctaccgc aatgctgatt aatcagttga taccctcgcc
    30661 attactggac tggctggccg gatacccggc ggcttttgcg tccctgattt accagctcac
    30721 cggacactac gaacggtctg gcgcactggc tgaactggtg cagcaggccg ataaagcctc
    30781 tgtcgcccga tacctcggcg gcgatatcaa aaccgcgctg gcgcaaaagc cctcttcctt
    30841 cccccagcaa cttcttacag ccttgcggtc gctggtacag aacgagtacc ggctcaataa
    30901 tcccgaatgc ggatccgatg gctggctgac ggaggatgcg ctgtggctta tcagcaaaac
    30961 caccgccgac cgggttcgcg cctggctgct ccagcatgga gtttccagcg tgccggaaaa
    31021 caataaccgc ctgtttgacg aactgctgac gcatcagctt attgttgcca acgaagataa
    31081 agcgatttgg cgctgcatta tccgatcgga taaaggctgg tcgcccgttg aaccgctgac
    31141 gctactgcgc ctgacgccgt ctgtaatctg ggggcaggat gatcaacgtc cggcctgttt
    31201 cgatggtgat gtaacgccgg taattgaagc cgaacccgct cctgaactta gcgcaccgac
    31261 tcaaccgtta ccggagatag cagacgatcc gaggcttggt gacaataaca atttcctgca
    31321 gtggctgaag aaccacacta gtccttcagc cacggtgaat acccggaacg gcaaggtaca
    31381 tgtggtggaa aaccatcttt tcttgatttc gccggggatc tttaaaacat acgcagcaga
    31441 aaccacaggc gatagcggcg acggctggaa actggcgcaa cgaatgtttc aggagtcagg
    31501 gctggccctg cgctgtaatg atgacagttt tatctggatc tgcgaggttc gggttccaca
    31561 aaagaaaagt agtcagttga aagggtttct gatggaagat ccgaagcagg tgttggggga
    31621 gaaagtgccg gtgaataatc cgtggctgaa gttgctgcgc gaatagctat ttatatcaaa
    31681 atactctctt ccaccgagct acagcgaatg ggctttaata cctaaagagc cgcatgatat
    31741 tcaataattg atttcaattc ttcaggcgaa aaaggcggat tacgccgatg caacatggcg
    31801 tggcaatttg ggcagacggg aataaaatct tttttgggat ccagagagta attctctttg
    31861 atgtctgata gcggaatcag gtggtgtata tggataaaac ctttcgctat ctcgccatac
    31921 accttcatca tatcaatacc acatactttg cacagacaac catgccaggc taatgcggcc
    31981 tgtcgggcct tacggtcgcg ttcatagcga ttaaccgtta cctgcgtagc tgcaccttca
    32041 gtaaatacgc tctgcggtgg cagttcatca ggaaaaatac tgatggcggg ctgctcgtta
    32101 cccaaatgtt tctcaacgat ctggaaccct tgatcggtaa gcaacttgac gcatttcgac
    32161 ttgataccac cactaaaatc ttcaggggaa aactcaacgc cagtcatatt tgaagcagca
    32221 ataccgacta tagctttcgg tggataactg cgcccttcaa agaccacatc atacagccgg
    32281 gactcgctga actggtggcg tacaccttca tcataggcac gcatagccgc aataatgcat
    32341 tccctggaaa gctggtctgg cagtttcttc ttcatttaca actcatcatc cctgaaacga
    32401 catttgctaa ctctttattt tatagcatat taatttatcc ggtgataaac agcactcttc
    32461 aatttcgcgt taccgggata ggggcatcag tgcagcggca acgtaacgta gcggcagaga
    32521 aatcgcgcct cattctggtt gtccagacgt acctgaaaga tatcgtgtgt atcctgcgcg
    32581 gatgcatcct cacgccacgc cagcagaccc cgttggttca tgcggataat aatctgatcg
    32641 ggcgtttcgc cctgattcaa tgcttcgact acctgttgta ggggaagcgg tagcgtctga
    32701 gcaggccaca tcacttcaac acttacgagc tggcggtgtg tccgaggtga catcggtggt
    32761 ttcctccatg gctggaattt agcggttaat tagccatgaa ataggggcat tttttactgt
    32821 ttcaactact gccgagatgg ctacggaata cgcgctgact gatgcaaaaa tagcttctct
    32881 tatgtgctct ggaaatccag atggtacgct agtgaaaatg gtggttccgt ccggatagcg
    32941 ccatggcctt tttgttcata tttccacaga tcaccactcc tgctcccagc catcagaaga
    33001 gtttttttac ccctccagct tacgacctga agcttaaggt ccagagcggc aagcagctta
    33061 aagagcgtat ccaggctggt cccctcagga tggttctcaa actctgaaac cgttgactgt
    33121 ttcatgccca tacttgctgc agtttcacgc tgggtcaggt tacgttgctt gcgtgcatcc
    33181 ctgattgcct gagacaacat tttgggtgag gttacgttca ttttatcccc caggatagat
    33241 attttgagca tatcccctat aggggataaa aattcatatt ctcagcggag aaaagattaa
    33301 taactaaatg aattaaaatt acttttcatg gaaattaagg ggggacgata ttcctcgagc
    33361 cagtaaatat gaacattgcc agatggcaga acgcgccatt ttacaagcat tttccataac
    33421 agatataaga ctgattgagc ggttggatta agcatgacga tatgtttaaa aaaaccagtt
    33481 ttatcgacat aacgccaaaa tatgtttaca gcatcgacat atcaacgcca tatgtacatt
    33541 ttttggcatt ttttaaacat atcatcagca ggctttattt tttgctatcg aaatctacag
    33601 tccctcgttt caatacaaaa attccagcgt gtcagctacc ttttctgggt ttctttattg
    33661 gttttagcag tgcagcaaga gatgcagatg tcacgacttc tgttttaact tcagaatcga
    33721 ttgatttttc ttgtgatgtc gagaattgct tgtcgaaggc atcgtttttt atcacgtttc
    33781 cggtatgcct gtttgtgtga gagatagcag caggacgaga aacacgcgtt ttatttatta
    33841 gccgggaatc ctgaagttgg cgtccctgtt catcctcagc ggcaagtttg ttcagatcct
    33901 tttcaagacc caaaatctgc ataactgaca aataagcgcc gattgtcacg cccgtcccgc
    33961 ctgattcaag agaacgcaag gtcattggcg acatcccggc tcgctcagca acccgcttcg
    34021 ctgtgagctt acgtcgcagt cgcgccagct taagccgttc gccaaactca gcaagcaact
    34081 gattagtacc gggaactaag ggagccgttt ttttagccat agtgataata ttttatcttt
    34141 tcaataccta tatagccata atattagcgc atcatgccga aggtgcggtg cgccgtggaa
    34201 ggtggaacat caagtttacc tgcgttctcc actatctttg atgggaacca gcaagccttt
    34261 gaccttcagt ttgatgatgt cgcgactgac tctgggctgg cattattgat aacataagcg
    34321 catcttaatc gggcagttgg cgttacgccc tgccattgtt cacaataata ccgttcatga
    34381 ttcatgaaca aaactctgtt gtgaacattt catctaaaac tacatcctca accatcactt
    34441 gccataccat cccagaaatc attgatgatc tgctcaatcc cagcatcacc cacgtcatgt
    34501 tgtcgtacta tcacatcgat caggcgttct ttatcaagat ggagtaaatc ctgcaaggat
    34561 gagtgttcac taattgtcgc tatatcgctt cccaacagaa aagcgcccat acgtatggga
    34621 tcccagttca ggcgttcccc ttcgacgggc gattcataga tgcgttcatt ctcgataaca
    34681 ccatattcac agataatctt aaagagatcg gtcgcgtctt tagtactccc atgcccacga
    34741 tcacgccatg caaaaagttt taacagagcc agtcctggca aggagcagaa cggaatattt
    34801 tcgctgttat ccagccgcac aactacggta tgagaaaaga cttcggcaaa tccatccaca
    34861 gccattatga tctcacgttc tggtggccag gcaatttgat ttccttcagc aacaccacca
    34921 aaaggaataa tatctagctc gatccctttg acaatcatac gatgggcatt accttggaca
    34981 acttctgccc cttcagcaag gaaagcattt ttaagcgcat caaatctcgg ccagctatcc
    35041 acaaagacgg caatatcaat atcacgggtt cggcgaccaa tacttctgct gtgtatatga
    35101 tggagcagaa tttcacgagc cgttgcacct gcaataaaaa acgggatccc ctgagcggtg
    35161 caaatacggt tgatctgact cagtaacgtt tcagtagctg aagagatcgg attcattaag
    35221 gtgaagatat ttgtcattga ttatccttgc tgtttcccag tttctgtcat ccccgcttgc
    35281 cagcagttca gccacgcata gcatcgcctc ggctttgtga ttcagtacaa agttccccca
    35341 aaaaccggcg atcagctgta gtttcccccc cggaacaggt tttagtccca gttcctttcg
    35401 cctttgtaac aacggatggg atgtaaaaat cacgccattt tctggtatca ggtatcctcc
    35461 gctcagcttt gccgccgcaa tctcgccgct ccagtattcc ccggacgcaa gcgtcaactc
    35521 atcccatgaa acaggcgcgg ataaagaaag cgaatccagc ttagggcgca aagctgtagc
    35581 ataatctttc agccacaaag cctctaactc gccttcattg attaagcgac gccccttctt
    35641 cgatttgcgg aaatagcgtt gcgcttcgag ataatcaaaa gccttgctga ccattcccag
    35701 agagatacct gcctttccgg ccagactgcg gtaagtctcg ttcagggtct ctgggctaga
    35761 cagcaaaaca aaaagaagct tcatcacccc ttcagcaaaa cgactgctgg cggaaacggg
    35821 tttcttttca tagcggcctt cgatccgcac atacaagcct ggaacctgga tacgggcatt
    35881 ccctgccgta tcgataaagt tgatctggct gtcggcgcaa tattccgcca gtgcagacgt
    35941 caggcggtta cacaccagca gcacaggaaa atccgcagga aagcgaacca tttgttcgcg
    36001 aacagccatc agtgactctt tacggtggat aaattttact tccagagcaa atatcactga
    36061 ctcaccatcc ggccctgtta gcttacccca ggcatcgtgc ttaccatgtc taccgctatg
    36121 ttcatactgc aaatggaatc cttttggcag attctctatc gcatcagaca aaagctgttc
    36181 ttccttcatt tgttcacacc tcataactgt tcatgaatca tgaacaaaac aatgttatga
    36241 acaaataaag aaagaagcaa cgtaaatgat caaaaaaaga ccgccagaaa tgcgatgcct
    36301 tattcaacct tccaaacgtc attttccatt aaagaaagaa gggtaaagag tagttggacg
    36361 cattctttca gggttgagta accattccta ctcccccgac tccacctcct cctgcgccgc
    36421 catcagaacc aactctgact tcgaaatcag caggtctttc ggcctcccac ctctcaacct
    36481 gcgattagcc gagccaaacc agatagccag cccccacggt gttttacgct ccttaaacag
    36541 tgaaagaatc aaacggacaa ccttgagagg ccgcattttc tcatccagaa cataatcagg
    36601 gtataaatcc tcaccatcga ccttcagcga gaatatcttc ccggcggctt tccagccttt
    36661 cggaccagca tcaggatcgg cggaacgaaa gcccgctttt gcggataatt cagacgccgt
    36721 cagccattcc gagtcagcca gtattctggc tttcagctcc tctagctgcc ggtcattgcg
    36781 ctcctggaaa ctcatatggt cgagtgcgcc tcccggcgac tttttgaagc catacggcac
    36841 aacatgcctg aaccggtttg catccagata atccgcccac cactgcatca tctcccaacg
    36901 ttcttcgaga tgctgcgcct tgtggatata cgccgcacgc acgcgcttgc gctcctgatg
    36961 gctcatctgg cgctctaccg cgtcgcttga ccacagcccc gactccacca gcgcgctaca
    37021 ggccatagtg cgaaaaccgt ggccacagac atctttctgg gtgtcgtagc ccatcacccg
    37081 cagcgctttg ttgatggtgt tttcactcat cggcttgcgg taattgtgat cgccggggaa
    37141 caccagttca gttcccggtc ggcaatgctg ccgcacctcg tgcagcagtt cgatggcctg
    37201 acgtgacaat gggaccaggt gtggggagtg catcttcgcc ccacgcgctg aatatttaac
    37261 gccggggatc ggctcacgct cagcggggat ggtccacatg gcgttacgca gatcaatttc
    37321 atcccagcgg gcaaagcgca gttcgctgga gcggataaac agcagcagtg ccagttttac
    37381 cgccagttga gtcagttgac ggcctttgta atcgtcgatt ctttccagga aatcagggat
    37441 aagattcaga tccaacgccg ggtgatgccg tactttgggc gtggagaccg cgccggttaa
    37501 atctgatgca gggttatgat cgataatgcc gttctggacg gcgtagcgca tcacgcaggc
    37561 agtgcgctgc tgaagccggg aagcaacatc cagtttgccc gctttctcca cttccttgat
    37621 gggcaccagc agatctttga ccttcatttt ggtgatgtca cagctgccgt tcgtggggaa
    37681 tacgtaaagc tcgaaataac gcagcacccg cttcttatgt tccgccgacc agttcacgtt
    37741 actggtgacc cattcacgtg ccacagactc aaacgtatgt tccggcgtta tcgcaaggcg
    37801 ttctgctttt cggcgtgcac tcggatcgat gccggaaatc agcaataatt gtgcctcttc
    37861 acgcatgcgt cttgcctgtg caagcgtcaa aagcggataa gggcctaaag cgagcttttt
    37921 ctctttgttt acaaagcggt acttgatgta ccagcgtttt gaaccgttag ggttaaccag
    37981 caggtaaagt ccatgcgagt cactgagctt atacggcttg ccacaaggct tggcgtggac
    38041 tatagcgcga tctgttagtg ccatttgggg gcctcactat tatcgaacta cgtctaagcc
    38101 cccaatttgg cccccaaatc gcgtggatgt caatggatga aaaatgacca acacggaaag
    38161 accacaaaat aaatatggtt attgtggaaa aataaattac ctttgaatta caaacggata
    38221 tggacgaatt tggacggaaa tggacacaaa aaaagccacc cttaggtagc taattattgc
    38281 atcacttggt gccgaaggcc ggactcgaac cggcacgtat ttctacggtt gattttgaat
    38341 caactgcgtc taccgatttc gccacttcgg cactgaagag gtatgcggaa aacgttgtgg
    38401 attatacctg tcgcacgcca ccatgcaagc gccagcttgc tcgcacctcg ctaagtgctg
    38461 aaattttcag cattgcatac cgcgatgtgt aacctttgtc acactccagg cgccccgccc
    38521 tgccatgctc tacacttccc agaccacacc agagaaggat aaaaacatgt cgatgataaa
    38581 aagttatgcc gcaaaagagg ctggcggcga actggaagtt tatgagtacg atcccggtga
    38641 actgaagcca caagatgttg aagttcaggt ggattactgc gggatctgcc attccgatct
    38701 gtcgatgatc gacaacgaat ggggattttc acagtatccg ctggttgccg ggcatgaggt
    38761 gattgggcgc gtggttgcat tgggtagcgc cgcgcaggat aaaggtttgc aggtcggtca
    38821 gcgtgtcggg attggctgga cggcgcgtag ctgtggtcac tgcgacgcct gtattagcgg
    38881 taatcagatc aactgcgagc aaggtgcggt gccgacgatt ttgaatcgcg gcggttttgc
    38941 cgagaagttg cgtgcggact ggcaatgggt aatcccactg ccagaaaata tcgacattga
    39001 atctgctggg ccgctgttgt gcggcggtat cacggtcttt aaaccactgt tgatgcacca
    39061 tatcactgcc accagccgcg tcggcgtgat tggtattggc gggctggggc atatcgctat
    39121 aaaacttctg cacgcaatgg gatgcgaggt gacagccttt agttctaatc cggcgaaaga
    39181 gcaggaagta ctggcgatgg gtgccgataa agtggtgaat agccgcgatc cgcaggcact
    39241 caaagcactg gcggggcagt ttgatctcat tatcaacacc gtcaacgtca gcctcgactg
    39301 gcagccttat tttgaggcgc tgacgtatgg cggtaatttc catacggtcg gtgcggttct
    39361 cacgccgctg tctgttccgg cctttacgtt aattgcgggc gaccgcagcg tctctggttc
    39421 tgctaccggc acgccttatg agctgcgtaa gctgatgcgc tttgccgccc gcagcaaggt
    39481 tgcgccgact accgaactgt tcccgatgtc gaaaattaac gacgctatcc agcatgtacg
    39541 cgatggtaaa gcgcgctacc gcgtggttct gaaagccgac ttctgatccc tttgccgggt
    39601 gcctgtcgcc cggcgtttaa atctctttgg aaggcgtctc gcaatctgct gaatttagcg
    39661 gtgaagagtg cgcagccgtt gcctatactc caggcaggaa actggaggat atctcatgag
    39721 cgaacccctg ttaattgccc gcacgtcgga cacagaactg tttttattgc cgggaatggc
    39781 gaatcgtcac gggctgatta ctggcgcaac ggggacgggt aaaaccgtta cgctgcaaaa
    39841 actggcagag tctctatctg aaatcggcgt gccggtgttt atggccgatg tgaaaggtga
    39901 tttaacggga gtagcggagg aaggtacttc ttcggaaaaa ctgctcgcaa ggcttaaaaa
    39961 tatcggcgta aatgactggc aacctcatgc taatccggtg gtggtgtggg atatctttgg
    40021 cgagaaaggc cacccggtgc gggcgacggt ttcggatctg gggccgctgt tgctggcgcg
    40081 actgttgaat ctcaacgatg tgcaatctgg cgtgctgaat attatcttcc gcattgcgga
    40141 cgatcaggga ttgttgctgc tcgactttaa agatttgcgg gcgattaccc agtacatcgg
    40201 cgataacgcc aaatccttcc agaatcagta cggaaatatc agtagcgcat cggttggtgc
    40261 catccagcgc ggattactgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc
    40321 gatgctggat atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat
    40381 cctcagcgcc gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgccgtggat
    40441 gctttcagag ttatatgaac aattgcctga agcgggcgat ctggagaaac caaaactggt
    40501 gtttttcttc gacgaagcac atctgctgtt taacgatgcg cctcaggtac tgctggataa
    40561 gattgagcag gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca
    40621 aaacccgtct gatattccgg ataatgtgct cgggcagctc ggtaatcgtg ttcaacacgc
    40681 tttgcgggct tttacgccca aagatcagaa agcagtaaaa gctgcggcgc aaaccatgcg
    40741 ggccaatccg gcatttgata ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt
    40801 aatctcgttt ctcgatgcaa aaggaagtcc ttctgtggtg gaacgggcga tggtgatcgc
    40861 accttgttcg cgaatggggc cggtgacgga agatgagcgt aatggcctga ttaatcactc
    40921 cccggtgtat ggcaaatatg aagatgaggt ggaccgcgag tccgcctatg agatgctgca
    40981 aaaaggcttt caggccagta ccgagcagca aaataatccc cccgtgaaag ggaaagaggt
    41041 ggcggtggat gacggtattc ttggtggatt gaaggatatt ttgtttggca ctaccggacc
    41101 acgcggcggg aagaaagatg gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt
    41161 gacgaatcag attgtacgcg ggatgttggg gagtttgctg ggggggagaa aaagataagt
    41221 cagacgtgat ctgtgcccct caccctaacc ctctccccaa aggggcgagg ggaccgttcg
    41281 aactcgtatt tgactttgtc aacaataata aagggagctt tcgctccctt gttagattac
    41341 gattttctca ttaacagcca caggctgatt aagaagaagc tggcgcttgg caacagtgcg
    41401 ccgatgatcg gcgggatgcc ataaaccaac gtcagcgggc cgaagatctg gtccagtacg
    41461 tagaagacaa aaccgaagct gataccggtg accacacgca cgcccatcgg tacgctacgc
    41521 agtgggccaa agatgaacga cagcgccatc agcatcatca ccgccacgga tagcggctgg
    41581 aagattttgc tccacatgtt gagctgataa cgtccggcat cctgaccgct cgacttcaga
    41641 tacttcacgt agttgtgcaa accactgatg gagagtgcat ccgggtccag cgccaccacg
    41701 cccagtttgt ctggcgtgag gttggttttc caggtgccgc tcaccgtctg cgaaccggta
    41761 atctgtttcg gattggtcag gtcagattca tcaacctgcg acagacgcca gactttatgt
    41821 tccgggtcaa acttcgcggt agcggcataa cgtacggatt gcagacgacg attctcgtta
    41881 aaggcataaa tactgatgcc acctaactcc tcgtcacctt taacccgctc aatgtagacg
    41941 aagttgttgc catctttcgc ccataagcct tgctgggtag agagcaacga gccgccgtac
    42001 atcgcctgcg cacggtagtt acgcgccatc tgctcgccct gcggcgcgac ccattcgcca
    42061 atcgccatcg tcagcaagac cagtggaatg gcggttttca tcaccgacag cgctacctgc
    42121 atacgggtaa aaccagaagc ctgcatcacc accagttcgc tgcgctgcgc cagcatccca
    42181 agaccaagca acgccccaag cagagccgcc atcgggaaga agatttgcac atctttcggc
    42241 acgctcagca aggtatacat tcctgcgcct aacgcgtcgt aactcccctg cccggctttt
    42301 ttcagctgat cgacaaactt gataatgccc gacagcgaca ccagcatgaa cagcgtcatc
    42361 atgatggtgg tgaaaatagt tttaccgata tagcggtcaa gtacgccaaa aggttgcatc
    42421 acaccgctcc tttacgcgaa aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa
    42481 ggttgagaac aatcgccaaa gccagataaa tcaggttaac ggtccacatc cacagcgtcg
    42541 ggtccagctt acctttaccg ccgttcgatt tcagggaggt ctggatcagg aagaaaagta
    42601 gatacagcag catggctggc agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc
    42661 tcagcggtac gaccataagt gccatcataa acacggtgaa taccaacgtg atacgccagt
    42721 tcagttctgc acgggcacga tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt
    42781 cggtatcgtt cgggtcgagc gccaccgcct ggtgaccaat gatcgcctga taatcctgga
    42841 agtccgtaat gcggaaatca cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga
    42901 gagtgacgac ctgggagccg tcgcgcagct gggttaaatg tccggaatcg gccaccacca
    42961 cagaaggacg tgcattacct tttggtcgaa tttgcgcgag gaacacatct ttgaaatcgc
    43021 tgccgtcaac gctttcgatg aacagcaccg agctgccatt agtcgcttgc tggaattgcc
    43081 cttgcgccag cgccgccatg ccagggttcg ctttcgcttc tgctaacact tcatcctgat
    43141 gacgcgatga ccacggtccc gcccacatca cgttaaccgc cgcgacgatt gccgtgaata
    43201 ccgcaaggat cattgccgct ttcaccagaa ccgctttgct caggccgcag gcatgcatta
    43261 ccgtaatttc actttcggta tacagtttgc ccagcgtcat cagcagcccg aggaacaggc
    43321 ttaatggcag gataagctgc gccatttccg gcacgcccaa cccgagaagg gagagcacca
    43381 gattcgccgg aatatcgccg tcaaccgctg cgccgaggat cctcactaac ttttgacaga
    43441 agaagatcaa aagcaagatg aagagtatcg ccagctggct tttgagcgtc tcccgcacca
    43501 gatatcttat gattatcact ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta
    43561 gcttgtttca tggcttaaac gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat
    43621 aattatactc aacggattca cctctcagat tttgttctga cgtgccaatg ccgtaataac
    43681 gttaagatta acacgaagtc atcgcaacag cggacatgag ttacgaaagc ttgcaattct
    43741 atctgtagcc accgccgttg tctttaagat tcaggagcgt agtgcatgga gtttagtgta
    43801 aaaagcggta gcccggagaa acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca
    43861 cgtcgccttt ctccgattgc agaacagctc gataaaatca gcgatgggta catcagcgcc
    43921 ctgctacgtc ggggcgaact ggaaggaaaa ccggggcaga cactgttgct gcaccatgtt
    43981 ccgaatgtac tttccgagcg aattctcctt attggttgcg gcaaagaacg tgagctggat
    44041 gagcgtcagt acaagcaggt tattcagaaa accattaata cgctgaatga tactggctcg
    44101 atggaagcgg tctgctttct gactgaactg cacgttaaag gccgtaacaa ctactggaaa
    44161 gtgcgtcagg ctgtcgagac agcaaaagag acgctctaca gtttcgatca gttgaaaacg
    44221 aataagagcg aaccgcgtcg cccgctgcgt aagatggtgt tcaacgtgcc gacccgccgt
    44281 gaactgacca gcggtgagcg cgcgatccag cacggtctgg cgattgccgc cgggataaaa
    44341 gcagcgaaag atctcggtaa tatgccgccg aatatctgta acgccgctta tctcgcctct
    44401 caggcgcgcc agctggctga cagctacagc aagaatgtca tcacccgcgt tatcggcgaa
    44461 cagcagatga aagagctggg aatgcactct tatctggcgg tcggtcaggg ttcacaaaac
    44521 gaatcgctga tgtcggtgat tgagtacaga ggcaacgcgt cggaagatgc acgcccaatc
    44581 gtgctggtgg gtaaaggttt aaccttcgac tccggcggta tctccatcaa gccttcagaa
    44641 ggcatggatg agatgaagta cgatatgtgc ggcgcggcgg cggtttacgg cgtgatgcgt
    44701 atggtcgcgg agctgcaact gccgattaac gttattggcg tgctggcagg ctgtgaaaac
    44761 atgcctggcg ggcgtgccta tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc
    44821 gttgaagtgc tgaacaccga tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac
    44881 gttgaacgtt ttgagccgga agcagtgatt gacgtggcga cgctgaccgg tgcctgcgtg
    44941 atcgcgctgg gtcatcatat tactggtctg atggcgaacc ataatccgct ggcccatgaa
    45001 ctgattgccg cgtctgaaca atccggtgac cgcgcatggc gcttaccgct gggtgacgag
    45061 tatcaggaac aactggagtc caattttgcc gatatggcga acattggcgg tcgtcctggt
    45121 ggggcgatta ccgcaggttg cttcctgtca cgctttaccc gtaagtataa ctgggctcac
    45181 ctggatattg caggaaccgc ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg
    45241 gtagcgttgc tggcacagtt cctgttaaac cgcgctgggt ttaacggcga agagtaagtt
    45301 tgcgttttac cctcacccta accctctccc taagggagag ggaaccgatc gagcgcaata
    45361 gaaattcatt gctcatcttt cagggtgagg aatgcagcaa tatttaaatc caccacaaga
    45421 agccccatat atgaaaaacg caacgttcta ccttctggac aatgacacca ccgtcgatgg
    45481 cttaagcgcc gttgagcaac tggtgtgtga aattgccgca gaacgttggc gcagcggtaa
    45541 gcgcgtactc atcgcctgtg aagatgaaaa gcaggcttac cggctggatg aagccctgtg
    45601 ggcgcgtccg gcagaaagct ttgttccgca taatttagcg ggagaaggac cgcgcggcgg
    45661 tgcgccggtg gagatcgcct ggccgcaaaa gcgtagcagc agcccgcgcg atatattgat
    45721 tagtctgcga acaagctttg cagattttgc caccgctttc acagaagtgg tagacttcgt
    45781 tccttatgaa gattctctga aacaactggc gcgcgaacgc tataaagcct accgcgtggc
    45841 tggtttcaac ctgaatacgg caacctggaa ataatggaaa agacatataa cccacaagat
    45901 atcgaacagc cgctttacga gcactgggaa aagcagggct actttaagcc taatggcgat
    45961 gaaagccagg aaagtttctg catcatgatc ccgccgccga acgtcaccgg cagtttgcat
    46021 atgggtcacg ccttccagca aaccatcatg gataccatga tccgctatca gcgcatgcag
    46081 ggcaaaaaca ccctgtggca ggtcggtact gaccacgccg ggatcgctac ccagatggtc
    46141 gttgagcgca agattgccgc agaagaaggt aaaacccgtc acgactacgg ccgcgaagct
    46201 ttcatcgaca aaatctggga atggaaagcg gaatctggcg gcaccattac ccgtcagatg
    46261 cgccgtctcg gcaactccgt agactgggag cgtgaacgct tcactatgga cgaaggcctg
    46321 tccaatgcgg tgaaagaagt tttcgttcgt ctgtataaag aagacctgat ttaccgtggc
    46381 aaacgcctgg taaactggga cccgaaactg cgtaccgcta tctctgacct ggaagtggaa
    46441 aaccgcgaat cgaaaggttc gatgtggcac atccgctatc cgctggctga cggcgcgaaa
    46501 accgcagatg gtaaagatta tctggtggtt gcgactaccc gtccggaaac cctgctgggc
    46561 gataccggcg tggccgttaa cccggaagat ccgcgttaca aagatctgat tggcaaatat
    46621 gtcattctgc cgctggttaa ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg
    46681 gaaaaaggca ccggctgcgt gaagatcacc ccggcgcacg actttaacga ctatgaagtg
    46741 ggtaaacgtc acgccctgcc gatgatcaac atcctgacct ttgacggtga tatccgtgaa
    46801 agcgcccagg tgttcgatac caaaggtaac gaatctgacg tttattccag cgaaatcccg
    46861 gcagagttcc agaaactcga gcgttttgcc gcacgtaaag cggtcgttgc tgcggttgac
    46921 gcactcggcc tgctggaaga aattaaaccg cacgacctga ccgttcctta cggcgaccgt
    46981 ggcggcgtag ttatcgaacc gatgctgacc gaccagtggt acgtgcgtgc cgatgtcctg
    47041 gcgaaaccgg cggttgaagc ggttgagaac ggcgacattc agttcgtacc gaagcagtac
    47101 gaaaacatgt acttctcctg gatgcgcgat attcaggact ggtgtatctc tcgtcagctg
    47161 tggtggggtc accgtatccc ggcatggtac gacgaagcgg gtaacgttta tgttggccgc
    47221 aacgaagacg aagtgcgtaa agaaaataac ctgagcgcgg acgtcgcgct gcgtcaggac
    47281 gaagacgttc tcgacacctg gttctcctcc gcgctgtgga ccttctccac gctcggctgg
    47341 ccggaaaaca ccgacgcact gcgccagttc cacccaacca gcgtgatggt atccggcttc
    47401 gacatcatct tcttctggat tgcccgcatg atcatgatga ccatgcactt catcaaagat
    47461 gaaaatggca aaccgcaggt gccgttccac accgtttaca tgaccggcct gattcgtgat
    47521 gacgaaggcc agaagatgtc caaatccaag ggtaacgtga ttgacccgct ggatatggtt
    47581 gacggtattt cgctaccgga actgctggaa aaacgtaccg gcaatatgat gcagccgcag
    47641 ctggcggaca aaatccgtaa gcgcaccgag aagcagttcc cgaacggcat tgagccgcac
    47701 ggtactgacg cgctgcgctt caccctggcg gcgctggctt ctaccggtcg tgacatcaac
    47761 tgggatatga agcgtctgga aggctaccgt aacttctgta acaagctgtg gaacgccagc
    47821 cgctttgtgc tgatgaacac agaaggtcag gattgcggct tcaacggcgg cgaaatgacg
    47881 ctatcgctgg ctgaccgctg gattctggcg gagttcaacc agaccatcaa agcgtaccgc
    47941 gaagcgctgg acagcttccg cttcgatatc gccgcaggca ttttgtatga gttcacctgg
    48001 aaccagttct gtgactggta tctggagctg accaagccgg taatgaacgg tggaacagaa
    48061 gccgaactgc gcggtactcg ccatacgctg gtgactgtac tggaaggtct gctgcgcctc
    48121 gcgcatccga tcattccgtt catcaccgaa accatctggc agcgtgtgaa agtactttgc
    48181 ggtatcactg ccgacaccat catgctgcag ccattcccgc agtatgatgc atctcaggtt
    48241 gatgaagccg cactggccga caccgagtgg ctgaaacagg cgatcgttgc tgtacgtaac
    48301 atccgtgcag aaatgaacat cgcgccgggc aaaccgctgg agctgttgct tcgtggttgc
    48361 agcgcggatg cagagcgtcg cgtaaatgaa aaccgtggct tcctgcaaac gctggcgcgt
    48421 ctggaaagca tcaccgtgct gcctgccgat gacaaaggtc cggtttccgt taccaagatc
    48481 atcgacggcg cagagctgct gatcccgatg gctggcctca tcaacaaaga agatgagctg
    48541 gcgcgcctgg cgaaagaagt ggcgaagatc gaaggtgaaa tcagccgcat cgagaacaag
    48601 ctggcaaatg aaggctttgt cgcccgcgca ccggaagcgg ttatcgcgaa agagcgtgag
    48661 aagctggaag gttatgcaga agcgaaagcc aagctgattg aacagcaggc tgttatcgcc
    48721 gcactgtaat caacacagtg aagaaaacga aggccggagc atgctccggc cttttttatc
    48781 tcttacagaa atggtaaaga aggcataact ccccgagaaa tactggctaa gaagccttta
    48841 tctggttgtt cttctttatt gattaaagga aggtcatcca aatcgcccag caaatacgta
    48901 ttttttgcct gccagtcaat catccatatt ttcagcagtg gataagccag accgcccgta
    48961 atcccggata tcaccaccaa cgcacacatg cgataaagca taccgtggta cgttaaagtt
    49021 gagcgaaaac gaatacgccc atcattcagt gacaggttgc tcataaaatg atttcgcaac
    49081 gacaccgtta aataactggt gctaacagca atcccaaaat aatagattaa ctgcgcgatt
    49141 atcattttac gttgcatttc cataaattgc tgtaaattct caatgtcatc atttgcatat
    49201 acagaactat catacgcatt taatatttga tcgaagataa tataaccagc aacagcgaga
    49261 aaaggcaata aagctaaaaa tgcgagaatg gcatatttta tacaatacgt agtatctaat
    49321 ttcaccttga aacgatgtat accgaaactg gtattgctcc agagaaagct cattaccagg
    49381 ctatataaag taccattaaa aataccaatg gaaacaatac caactattgc catcagaacc
    49441 acagatataa taacactact tgaactattg gcaggtagca tctttgtcga gataaagaaa
    49501 acagtcccca tcccaatggc cattaaaatc ggcaagaaaa aggttaccca ccagaaccct
    49561 ttcaaagagc aattaaaact aaatcttacg ccgttgagac tggtcattaa ggcctgataa
    49621 cgcagtcctt ttgctgccat aaatataaga aaaaccaaca gtaacaaagt caaaacacag
    49681 ccaacaagcg gcatatctgc tgacactgtc attaagattg cgaaatagaa aaaaacaaaa
    49741 acaagacaac taacaaaaac attcccaccg gtaattccat aagaaaatcg ttgtccgtta
    49801 acttccatat tagcataaag ataacgctta catttcatta atgcccatgg taaatagata
    49861 cctagcgtta taatcgttaa caacacattc accacacaaa taagaaaata tctctcacct
    49921 gtaccatgaa aaacaaaaga atgggacgga acatccattt cattaataac ttgagccata
    49981 aaaattccct gaattaaaaa atccattgat ataatttata gatacagaca gtaaaatgtt
    50041 ctgccacgtt acaatactct tatcagtaat aggctggcaa aaactttatt gctttataag
    50101 acgcttgcgc acgtaagaag agagtggtat taaacaaatg tataattact ttatttttat
    50161 tgggttaaac tatgaataac attgcgccgc aatcacctgt aatacgtcgc ctgacgctgc
    50221 aggataatcc tgctatcgcc cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg
    50281 ctgataaagg ctacaccgtc gccgatccga atcttgacga gctgtatcaa gtgtatagtc
    50341 agcctggcca tgcatattgg gtggttgagt acgagggtga agtggtcggc ggcggtggga
    50401 tagcgccatt aacaggtagt gaagcggaca tttgcgaact gcagaaaatg tactttcttc
    50461 ccgccattcg cggtaaaggg ctggcaaaaa aactggcctt aatggcgatg gagcaggcgc
    50521 gagaaatggg tttcaaacgc tgctatctgg aaacgaccgc ttttttgaaa gaagcgattg
    50581 gtttatatga acatttaggt tttcaacata tcgactatgc acttggctgc acgggccatg
    50641 ttgattgtga agtgcggatg ttgcgcgaac tctgaaaaaa tgcccgctag tatgtgctat
    50701 cgggcatttt aatcaattaa ttagtggcga accccgtcat cgtcttcatc gacaaaatct
    50761 tcatcgtcgc catcttcgcc gttaggatct tcaaagtaag tgccccaacc gtcgtactca
    50821 acgtcaaatt tctccgccag cgtcatcagt tgttcaacct gggcatcgat cagatcggca
    50881 ttcaacgcgc actcgctgag gatgtcgcag caaatcacga tatcaccatc ttcaacttcc
    50941 agctcttctg gatcggtcac ttcgtaaccg agtttaaacg cttcaactgc cgctttttcc
    51001 agagtttcca gatcgtctgc ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg
    51061 ctgccatctt ccagtaattc ttcaataatc aaacgtgttt cttcacgctg ttcttccagt
    51121 tgttccgggt ttgccatggc tcattcctct taatgtcctg ccgatactct tattgtcaca
    51181 caccactgac attgcctcca cctttgtgat aaagatttat gctttagact tgcaaatgaa
    51241 taatcatcca tataaattga attttaattc attgaggcgt tagccacagg agggatctat
    51301 gtccgggttt tatcataagc atttcctgaa attactcgat ttcacgccag ctgaacttaa
    51361 cagcctgctg cagttagccg cgaagctgaa agctgataag aaaagcggta aagaagaagc
    51421 caaactcact ggtaaaaaca tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg
    51481 ctctttcgaa gttgccgcat atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg
    51541 tagtcagatt ggtcataaag agtcgattaa agacactgcc cgcgtgcttg gtcgcatgta
    51601 tgacggtatt cagtatcgcg gctatggtca ggagattgtc gaaacactgg cggaatacgc
    51661 tggcgtgccg gtatggaacg gcctgaccaa tgagtttcac cccacgcagt tgctggcgga
    51721 tctcctcacc atgcaggagc atttacccgg taaagcattc aacgaaatga cgctggtcta
    51781 tgcaggtgac gcacgtaaca acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg
    51841 tctggatttg cgtctggtcg cgccacaggc atgctggcca gaagctgcgc tggttacgga
    51901 atgccgcgcc ctggcacaac aaaatggcgg gaatattacg ctgacggaag atgtagctaa
    51961 aggagttgaa ggtgctgact ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa
    52021 agaaaaatgg gctgaacgca ttgcattact gcgtgattat caggtgaaca gcaagatgat
    52081 gcagttgacc ggtaacccgg aggtcaaatt cctccactgc ctgcccgcgt ttcatgacga
    52141 ccagacgacg cttggtaaga aaatggcgga agagtttggc ctacatggcg gaatggaagt
    52201 gactgatgag gtcttcgaat ctgccgccag cattgttttt gatcaggcgg aaaaccgcat
    52261 gcataccatc aaagcggtga tggtc
//
LOCUS       DAZGTJ010000026        50509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000026 DAZGTJ010000000
VERSION     DAZGTJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.026"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..677
                     /gene="comR"
                     /locus_tag="ACHK8E_001908"
     CDS             45..677
                     /gene="comR"
                     /locus_tag="ACHK8E_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP0344183.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(739..1278)
                     /gene="ycfJ"
                     /locus_tag="ACHK8E_001909"
     CDS             complement(739..1278)
                     /gene="ycfJ"
                     /locus_tag="ACHK8E_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0344184.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1505..2809)
                     /gene="ndh"
                     /locus_tag="ACHK8E_001910"
     CDS             complement(1505..2809)
                     /gene="ndh"
                     /locus_tag="ACHK8E_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP0344185.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3209..3751)
                     /gene="ycfP"
                     /locus_tag="ACHK8E_001911"
     CDS             complement(3209..3751)
                     /gene="ycfP"
                     /locus_tag="ACHK8E_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP0344186.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3774..4799)
                     /gene="nagZ"
                     /locus_tag="ACHK8E_001912"
     CDS             complement(3774..4799)
                     /gene="nagZ"
                     /locus_tag="ACHK8E_001912"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP0344187.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4810..5634)
                     /gene="thiK"
                     /locus_tag="ACHK8E_001913"
     CDS             complement(4810..5634)
                     /gene="thiK"
                     /locus_tag="ACHK8E_001913"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP0344188.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5615..6256)
                     /gene="lpoB"
                     /locus_tag="ACHK8E_001914"
     CDS             complement(5615..6256)
                     /gene="lpoB"
                     /locus_tag="ACHK8E_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP0344189.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6270..6647)
                     /gene="ycfL"
                     /locus_tag="ACHK8E_001915"
     CDS             complement(6270..6647)
                     /gene="ycfL"
                     /locus_tag="ACHK8E_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP0344190.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6650..7009)
                     /gene="hinT"
                     /locus_tag="ACHK8E_001916"
     CDS             complement(6650..7009)
                     /gene="hinT"
                     /locus_tag="ACHK8E_001916"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP0344191.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7343..9532
                     /gene="fhuE"
                     /locus_tag="ACHK8E_001917"
     CDS             7343..9532
                     /gene="fhuE"
                     /locus_tag="ACHK8E_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP0344192.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9592..11025)
                     /gene="ptsG"
                     /locus_tag="ACHK8E_001918"
     CDS             complement(9592..11025)
                     /gene="ptsG"
                     /locus_tag="ACHK8E_001918"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP0344193.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11320..12117)
                     /gene="ycfH"
                     /locus_tag="ACHK8E_001919"
     CDS             complement(11320..12117)
                     /gene="ycfH"
                     /locus_tag="ACHK8E_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0344194.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12128..13132)
                     /gene="holB"
                     /locus_tag="ACHK8E_001920"
     CDS             complement(12128..13132)
                     /gene="holB"
                     /locus_tag="ACHK8E_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP0344195.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13129..13770)
                     /gene="tmk"
                     /locus_tag="ACHK8E_001921"
     CDS             complement(13129..13770)
                     /gene="tmk"
                     /locus_tag="ACHK8E_001921"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0344196.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13760..14782)
                     /gene="yceG"
                     /locus_tag="ACHK8E_001922"
     CDS             complement(13760..14782)
                     /gene="yceG"
                     /locus_tag="ACHK8E_001922"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP0344197.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14785..15594)
                     /gene="pabC"
                     /locus_tag="ACHK8E_001923"
     CDS             complement(14785..15594)
                     /gene="pabC"
                     /locus_tag="ACHK8E_001923"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP0344198.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15714..16955)
                     /gene="fabF"
                     /locus_tag="ACHK8E_001924"
     CDS             complement(15714..16955)
                     /gene="fabF"
                     /locus_tag="ACHK8E_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP0344199.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17043..17279)
                     /gene="acpP"
                     /locus_tag="ACHK8E_001925"
     CDS             complement(17043..17279)
                     /gene="acpP"
                     /locus_tag="ACHK8E_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0344200.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17490..18224)
                     /gene="fabG"
                     /locus_tag="ACHK8E_001926"
     CDS             complement(17490..18224)
                     /gene="fabG"
                     /locus_tag="ACHK8E_001926"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0344201.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18237..19166)
                     /gene="fabD"
                     /locus_tag="ACHK8E_001927"
     CDS             complement(18237..19166)
                     /gene="fabD"
                     /locus_tag="ACHK8E_001927"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0344202.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19182..20135)
                     /gene="fabH"
                     /locus_tag="ACHK8E_001928"
     CDS             complement(19182..20135)
                     /gene="fabH"
                     /locus_tag="ACHK8E_001928"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP0344203.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20203..21273)
                     /gene="plsX"
                     /locus_tag="ACHK8E_001929"
     CDS             complement(20203..21273)
                     /gene="plsX"
                     /locus_tag="ACHK8E_001929"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP0344204.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21354..21527)
                     /gene="rpmF"
                     /locus_tag="ACHK8E_001930"
     CDS             complement(21354..21527)
                     /gene="rpmF"
                     /locus_tag="ACHK8E_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP0344205.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21579..22100)
                     /gene="yceD"
                     /locus_tag="ACHK8E_001931"
     CDS             complement(21579..22100)
                     /gene="yceD"
                     /locus_tag="ACHK8E_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP0344206.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22299..22883
                     /gene="yceF"
                     /locus_tag="ACHK8E_001932"
     CDS             22299..22883
                     /gene="yceF"
                     /locus_tag="ACHK8E_001932"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP0344207.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="ACHK8E_001933"
     CDS             complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="ACHK8E_001933"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP0344208.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24528..27713
                     /gene="rne"
                     /locus_tag="ACHK8E_001934"
     CDS             24528..27713
                     /gene="rne"
                     /locus_tag="ACHK8E_001934"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP0344209.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            27847..28140
                     /locus_tag="ACHK8E_001935"
     CDS             27847..28140
                     /locus_tag="ACHK8E_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0344210.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            28137..28631
                     /locus_tag="ACHK8E_001936"
     CDS             28137..28631
                     /locus_tag="ACHK8E_001936"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0344211.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(28726..29679)
                     /gene="flgL"
                     /locus_tag="ACHK8E_001937"
     CDS             complement(28726..29679)
                     /gene="flgL"
                     /locus_tag="ACHK8E_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0344212.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(29691..31334)
                     /gene="flgK"
                     /locus_tag="ACHK8E_001938"
     CDS             complement(29691..31334)
                     /gene="flgK"
                     /locus_tag="ACHK8E_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP0344213.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(31400..32341)
                     /gene="flgJ"
                     /locus_tag="ACHK8E_001939"
     CDS             complement(31400..32341)
                     /gene="flgJ"
                     /locus_tag="ACHK8E_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP0344214.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(32341..33438)
                     /gene="flgI"
                     /locus_tag="ACHK8E_001940"
     CDS             complement(32341..33438)
                     /gene="flgI"
                     /locus_tag="ACHK8E_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0344215.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(33450..34148)
                     /gene="flgH"
                     /locus_tag="ACHK8E_001941"
     CDS             complement(33450..34148)
                     /gene="flgH"
                     /locus_tag="ACHK8E_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0344216.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(34201..34983)
                     /gene="flgG"
                     /locus_tag="ACHK8E_001942"
     CDS             complement(34201..34983)
                     /gene="flgG"
                     /locus_tag="ACHK8E_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0344217.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(35154..35909)
                     /gene="flgF"
                     /locus_tag="ACHK8E_001943"
     CDS             complement(35154..35909)
                     /gene="flgF"
                     /locus_tag="ACHK8E_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP0344218.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35929..37134)
                     /gene="flgE"
                     /locus_tag="ACHK8E_001944"
     CDS             complement(35929..37134)
                     /gene="flgE"
                     /locus_tag="ACHK8E_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0344219.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(37159..37854)
                     /gene="flgD"
                     /locus_tag="ACHK8E_001945"
     CDS             complement(37159..37854)
                     /gene="flgD"
                     /locus_tag="ACHK8E_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP0344220.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37866..38270)
                     /gene="flgC"
                     /locus_tag="ACHK8E_001946"
     CDS             complement(37866..38270)
                     /gene="flgC"
                     /locus_tag="ACHK8E_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0344221.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(38274..38690)
                     /gene="flgB"
                     /locus_tag="ACHK8E_001947"
     CDS             complement(38274..38690)
                     /gene="flgB"
                     /locus_tag="ACHK8E_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP0344222.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38846..39505
                     /gene="flgA"
                     /locus_tag="ACHK8E_001948"
     CDS             38846..39505
                     /gene="flgA"
                     /locus_tag="ACHK8E_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0344223.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            39581..39874
                     /gene="flgM"
                     /locus_tag="ACHK8E_001949"
     CDS             39581..39874
                     /gene="flgM"
                     /locus_tag="ACHK8E_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0344224.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39879..40295
                     /gene="flgN"
                     /locus_tag="ACHK8E_001950"
     CDS             39879..40295
                     /gene="flgN"
                     /locus_tag="ACHK8E_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP0344225.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(40335..41870)
                     /gene="murJ"
                     /locus_tag="ACHK8E_001951"
     CDS             complement(40335..41870)
                     /gene="murJ"
                     /locus_tag="ACHK8E_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP0344226.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41980..42903)
                     /gene="yceM"
                     /locus_tag="ACHK8E_001952"
     CDS             complement(41980..42903)
                     /gene="yceM"
                     /locus_tag="ACHK8E_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0344227.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(42905..43552)
                     /gene="yceH"
                     /locus_tag="ACHK8E_001953"
     CDS             complement(42905..43552)
                     /gene="yceH"
                     /locus_tag="ACHK8E_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP0344228.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(43563..44147)
                     /gene="rimJ"
                     /locus_tag="ACHK8E_001954"
     CDS             complement(43563..44147)
                     /gene="rimJ"
                     /locus_tag="ACHK8E_001954"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0344229.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            44383..45591
                     /gene="mdtH"
                     /locus_tag="ACHK8E_001955"
     CDS             44383..45591
                     /gene="mdtH"
                     /locus_tag="ACHK8E_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP0344230.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            45655..46302
                     /gene="grxB"
                     /locus_tag="ACHK8E_001956"
     CDS             45655..46302
                     /gene="grxB"
                     /locus_tag="ACHK8E_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP0344231.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            46436..46996
                     /gene="yceB"
                     /locus_tag="ACHK8E_001957"
     CDS             46436..46996
                     /gene="yceB"
                     /locus_tag="ACHK8E_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0344232.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            47102..48148
                     /gene="pyrC"
                     /locus_tag="ACHK8E_001958"
     CDS             47102..48148
                     /gene="pyrC"
                     /locus_tag="ACHK8E_001958"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0344233.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            48222..48467
                     /gene="dinI"
                     /locus_tag="ACHK8E_001959"
     CDS             48222..48467
                     /gene="dinI"
                     /locus_tag="ACHK8E_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP0344234.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48757..49011
                     /gene="bssS"
                     /locus_tag="ACHK8E_001960"
     CDS             48757..49011
                     /gene="bssS"
                     /locus_tag="ACHK8E_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP0344235.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            49126..50244
                     /gene="solA"
                     /locus_tag="ACHK8E_001961"
     CDS             49126..50244
                     /gene="solA"
                     /locus_tag="ACHK8E_001961"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP0344236.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            50292..50405
                     /locus_tag="ACHK8E_001962"
     CDS             50292..50405
                     /locus_tag="ACHK8E_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP0344237.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
BASE COUNT        12241 a        13591 c        12538 g        12139 t
ORIGIN      
        1 tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca actgactcaa
       61 cacaatgtgt aaaaaaaagc cgcggccgcc caaaagtgtt cgacagggat gccgcgcttg
      121 ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt gcggacctcg
      181 tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac aaagaggggt
      241 tattccgcgc cgtactcgac cgctatatcg atcgttttgc cgctaagcat gaagcacagc
      301 tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct gccatcgcca
      361 actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac tgcaccaccc
      421 tctccccaga ttcaggagat atcgccaata cgctgaaatc acgccatgcg atgcaagagc
      481 gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg acccactgtg
      541 acgtgacgca tctggcagaa ttccttaatt gtattattca ggggatgtcg atcagcgcac
      601 gcgaaggtgc atcgctggaa aaactgatgc agattgcccg aacgactttg cgtttatggc
      661 ccgaactggt gaaataaggc gcaaaaaaag cccctcagcc tgagcgaatg aggggccaaa
      721 ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt tgctatctag
      781 cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat cgccaatctt
      841 ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt tacaacgctg
      901 ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt taccggcata
      961 tccaccaccc agcgccccca caacagtagc gacatcttta ccgcgaccac caccaaactg
     1021 atgccctatc acgccgccag caacagcgcc gagcaccgac ccggtaatgc gattttcatc
     1081 ctgcaccggt cgacgatggg tcactgtgac gttacgacac tcctgacgcg gtgttttaac
     1141 cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggccac gttcaaacac
     1201 gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc cgatacccgc
     1261 caacattgat ttattcacgg gaatacctcc ttctttgcag ttatgccaat catgcaagcg
     1321 aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatggctg gaaatactta
     1381 gggattcgga ggagtctgaa ggaatgcctg atgcgacact taacgcgtct tatcaggcct
     1441 accgctcgta ccatttgtcc agggcggata aggcgtttat gccccatccg ccagtacaag
     1501 ccgattaatg caacttcaaa cgtggacgga taacgcggtt aatactcccc accagcatca
     1561 ttaatccagt tttaaagtaa ccatgcagcg caatctgatg cattcggtat agcgagatat
     1621 atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgtgtcagg ttacccatca
     1681 ggctaccgac ggtggaaaag ttcgacagcg ataccagcga accgtgatct ttatactgat
     1741 aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg caggttgcca
     1801 tctgatgtgc ggcctgagcg cgcggcggaa caaagccccc ttccggacgc gggcatgacg
     1861 cgcagtcgcc aatagcgtat atgtctggat cgcgagtggt ttgcagcgtc ggttccacca
     1921 ccagctggtt gatacggttg gtttcaagac caccgatatc tttcaggaag tctggcgctt
     1981 tgatcccggc tgcccacacc atcagatcag cctcaatata ttcgccatct ttagtgtgca
     2041 ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg ccaagtttcg
     2101 ttaactcact atgggccgca gcagagatac gtggcggcaa tgcaggcaaa atacgttcgc
     2161 ccgcttctac cagcgttacg ttcagggctt cgttggtcag acctttataa ccgtaactgt
     2221 gcagttgctt gaccgcgttg tgcaattcag cggagagttc tacacccgtc gcgccgccgc
     2281 cgacaatcgc aatgtttact ttgccattcg cgcccaggtt ggcggagtat ttcaggaaca
     2341 gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg aaaatgcagt
     2401 tctctttgac acctggcgta ttgaaatcgt tggaggtgct acccagcgcc attaccaggg
     2461 tgtcataggc gattttacgt tccggaacca gcaattcacc tttttcgtcg cgcagttctg
     2521 caatagtgat tgttttcgct tcacgatcaa tatcaattac ggaacccagc tggaactgga
     2581 aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca tcaagcgagc
     2641 cagtcgccac ttcgtgcagc agcggtttcc acaggtggct gtggttacga tcgaccagcg
     2701 taattttggc ttttttcttg cgtcccagct tatgccccag ctgtgttgcc atttccagcc
     2761 caccagcacc gccgccgaca atcacaattt ttttcaatgg cgtagtcaac gtgaccccct
     2821 taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat taacaacagg
     2881 ttacagcaat gaaaatgttc ttcactattg agaataacat gaatggtgca ttggtcatac
     2941 caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac cgggcgagat
     3001 caaaaaacag agtttattgt ttattatttg agcgaatttg tgagagtgat atagagtgag
     3061 gggtgtatgg agtgcgttcg gtaggcctga taagaggcgc atcaggcatt gatgctcgac
     3121 tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca tcaggtaccg
     3181 gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgcc ttaatgcgct
     3241 gcaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
     3301 aataatgatg cagctcttca gatgtccgct ggctgttaag agcttcatca ttacgcgaca
     3361 aaatcaccaa acaacgatcg cgattctttt cgcggaagtt ggtcacacac ttagtggcaa
     3421 tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat
     3481 tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc
     3541 cgcccagacc aacgccgcaa atcagcggac gctcgtcgac gttcagttgc aacattttgt
     3601 ccacttcttt gagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca
     3661 agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt
     3721 tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga
     3781 cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggcgct gatcgctttc
     3841 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
     3901 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
     3961 ttgcagacca ggatcatatc gcaacccgcg tccagtgatg cctgcccgcg ttcggcataa
     4021 ctgcccataa tcgcggctcc ttccatcgat aaatcgtcag agaaaatcac gccatcaaaa
     4081 cccagttcct gacgcaaaac ggttttcagc caatagggag aaccgctcgc cggacgcgga
     4141 tcgacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
     4201 aaggaactga agaccgacat atctttagcg cgaatctccg cttgcggacg tgggtcgcac
     4261 ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc
     4321 gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt
     4381 tcgggatcgg aatgataaga acgctcgcca atcgccgcgc tgatatgccc gacgtccagc
     4441 actggcgcaa agctgatatc aatatccata gcaatcattt cgctggccat caaccaacct
     4501 gcctctcgcg ccagttttcc gccttcttcc attcccaaca gcgcagcaaa tgattgtgcc
     4561 gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc ctgatcaacc
     4621 gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc acgtaactgg
     4681 gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgccagt
     4741 atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac tggacccaca
     4801 ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa
     4861 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag
     4921 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
     4981 ggcatagtca ttgaccaatt gccggtgctg gtcagtattt tccacccaca ccgccgccag
     5041 ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc
     5101 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg
     5161 tggttcccgc gctttgcgca gacgttttaa ccttcgcagc caacccactg tccgccgcgc
     5221 cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc gccagccaaa
     5281 acgcggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
     5341 cgtttttacc tcgccgggca gatagtcgac caccatccag tcacggagat ataaatgcgg
     5401 cttcggtgca atgcatgcgg gtagttgtga taaagcccga tactggcgca agaacgcgaa
     5461 ccgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
     5521 gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
     5581 aaagcgcgac agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg gcacctttac
     5641 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgaagg gtcggagcgt
     5701 taacattgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc
     5761 ctatggcttt actacgggtg cctaaactgt cctgcggcga caaacctaac tgttgctttg
     5821 ccatcgacag ctgctgggcg gaaaccaggg taaatttacc gttattagcc agcgcatttc
     5881 gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat
     5941 caaccagcag gacgctaccc gcagtcacgc cgtcagcccc aagcatctta ctgaccatcg
     6001 gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt gcagtttgat
     6061 cttcgtgctc aattgggcct ggctgctgcg gtatcgtcgg caccgatggc actgtgggga
     6121 caggctgttg cggctcggct ggttgttccg gcgctggttt cacttcttct accggcgcag
     6181 gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc
     6241 gactcatttt ggtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc
     6301 gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg aagggtaacg
     6361 ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga
     6421 taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga
     6481 atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac
     6541 tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc
     6601 agcactaaca acgccagaga caccagccca aagcatcctt ttctcattgt tacagacctt
     6661 tatgcgccag cattggtccc agtggacggc cacccaacaa atgcatatgg atgtggtaaa
     6721 cctcttgtcc gccatggcgg ttggtgttca tgatcaggcg atagccatct tcggcaatac
     6781 cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct
     6841 cagctgacac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg
     6901 gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg
     6961 aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg acattttcct
     7021 ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat
     7081 ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt
     7141 tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt
     7201 ctctgtttgg tttacacgtc aaatatgaat gcgtatattt ctcatttgca tttacatgca
     7261 aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac
     7321 aacataaacc aagagatttc agatgctttc aacacaattt aacagggata atcaatatca
     7381 aaccacctcc aaaccgtcac tgcttgccgc ctgcatagca ctggcactct taccttccgc
     7441 cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccactactcc
     7501 agttgatgac gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
     7561 gactcaacgt gatattcctc agtcggtcac tattgttagt cagcagcgga tggaagatca
     7621 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagccaagc
     7681 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
     7741 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
     7801 actgtttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
     7861 tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga
     7921 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
     7981 actcaccgaa gatggtaaaa tccgcgcacg aattgtcggc ggctaccaga ataacgactc
     8041 atggctggac cgctacaaca gtgaaaagac cttcttctcc ggcattgtcg atgctgattt
     8101 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
     8161 aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgcgc
     8221 acgcagtacc gcacctgact gggcgtataa cgataaagag atcaacaagg tctttatgac
     8281 cctcaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actccgaagt
     8341 cgaatttgac agtaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
     8401 ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggta ccggttggaa
     8461 cagcggcaaa cgtaaagttg atgcgctgga tttgtttgct gacggtagtt atgaattgtt
     8521 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttactt
     8581 cagttcatgg gcctacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
     8641 tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca cacatatgaa
     8701 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
     8761 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
     8821 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
     8881 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
     8941 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
     9001 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
     9061 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggtgtgga
     9121 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acgtttggcg caacgcgcta
     9181 tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
     9241 aatgttcacc agctaccggt tgcctgtcat gccagagttg acggtcggcg gtggtgttaa
     9301 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
     9361 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
     9421 acaggggaat gtcaataacc tgttcgacaa aacctacgat accaacgtgg aaggttctat
     9481 tgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
     9541 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
     9601 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
     9661 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
     9721 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
     9781 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
     9841 cgcttttgcg tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
     9901 cagcacgcgg aagatggtgt agtaaacaat ggcataaccg ataccgacga tcgggaacag
     9961 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    10021 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    10081 gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat
    10141 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt
    10201 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    10261 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    10321 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    10381 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    10441 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    10501 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggcctgaaat
    10561 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    10621 gaaacggtta aacatgtacg ctgcgatcgc accggagata atccccccga gtacgccagt
    10681 atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    10741 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    10801 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    10861 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    10921 cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg gcagcatcag
    10981 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    11041 tgagtatggg tgcttttttt acgttttcac gcgtggcaag gggggagagc ctcgccgtgt
    11101 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccgatttg
    11161 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    11221 atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttaggcgg tgaactttac
    11281 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    11341 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    11401 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    11461 attctctttt cctcgatgcg gaaccggtgc aaggtaaggt gagtcagttt ctaccagtaa
    11521 ccgatcctgg gggacataac gcgcggcatc gcgcagttgc tccgcattac ggaaggtcac
    11581 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    11641 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    11701 cagcgtatcg gcgcgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    11761 aatctggata tgatggatga aggattcttg ctgccgtact ttagtttccg gcgtgtaata
    11821 ataatccaac ccggtttcac ccagcgcaac aacgccctct tctgccgcca gacggcgtaa
    11881 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    11941 gttgtcacgt tcgcctacca gatcccgcat gtgtaaataa cccggtaatg ttgtggcgac
    12001 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    12061 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    12121 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    12181 gcagtaaaag atcggtgatg agaagctcgc ggttaatacc tgtgacagac attaactgtt
    12241 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    12301 ccagttcgac gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    12361 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    12421 gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat gccaaagcct
    12481 gacacaatgt ttcacgagcc tgccagttat ctccgtgaaa caacgccagt gccgcgccag
    12541 gcgaaccggc acttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    12601 gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt aaccgacaac
    12661 gactacgtaa tgttgccagt aaacgttctg gctcgcgggt agcaaggaaa aaccacgttt
    12721 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    12781 cagcatcggt tacccagaca actttcgcac cacctaagcg tgcgtgctca ttcagctttt
    12841 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    12901 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    12961 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    13021 catcgcccat gcccggtaac gcctgaatga gcagcgcatg gtgacctctt ccggcctgat
    13081 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    13141 ctccttcacc cagttggtca cggtagtgcg gattgcatcc atcacagcct ccagcggcag
    13201 ggtggcatca atggtatgaa tgcttttatc ttgtgctgcc agttccagat agcgggcgcg
    13261 ggtgcgatta aaaaaatcga aagactcttg ctcaatacga tccagctcgc cgcgtgcacg
    13321 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa
    13381 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtccgcc
    13441 gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccaag tgccgttagc
    13501 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    13561 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    13621 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    13681 cagttgctca agcgtctcaa ccaccacgtt acgcgcggta gttttgcctg cgccttccag
    13741 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    13801 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    13861 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    13921 ccggcgtcgc tatcgcacct ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    13981 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    14041 cggtcgggtc ggtctgcagg cgcataccaa tgcgtaaccg gttgataaat accgaggcaa
    14101 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    14161 actggttttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    14221 ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta ttggcggtat
    14281 acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    14341 cctgcgctac ggtggcgtac ttatcgtcgc tcagcgtgtg cttgatatag ggggcctcac
    14401 gcaattgctt gaggtaatcg ctcagacgca tcccttctac cagtcgcaga gggaactgtg
    14461 cttctttacc gctttccagc aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    14521 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattggaaaa
    14581 cccgtgggcg attgatgatc ttatcggcat aaagctgttc accgagcgcc agacgtccgg
    14641 tccctggctt cagggtaaat atcgtctctt ctttgataag taatttgctg tcggcaagat
    14701 ggcgaagctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    14761 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    14821 atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat
    14881 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    14941 ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc
    15001 gttaacacct gcctgatcca gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa
    15061 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    15121 gtttgtctgc tcaagatgag agcggatcaa tacttgctca agccgattaa gatgtttaat
    15181 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccctc
    15241 gttacgcaaa cggtcgtaat gtgcgggata aaccgtaacg gagagaatcc gcgttgccgg
    15301 tccgctgttc aatgtgctgt atcctcgccc gccactaccg cgactgatca ccactttcag
    15361 cacaccattt tgctgttctg ctgccagcgt tttcatctct agttcaagct gaggccagaa
    15421 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa
    15481 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    15541 tgcccgatcg cttactgcca gcgattcctg cttatgaccg ttaattaaga acataccggc
    15601 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    15661 gctaagaaaa aaggcccgca agcggacctt ttataagggt gggaaatgac aacttagatc
    15721 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    15781 tccattccgc tgacctgacg tgcttcgtgc ggtacgaaat ccagatcgca accttcatcc
    15841 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    15901 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    15961 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    16021 tcgccagccg gcgtagaagt accgtgagcg ttaacgtagc caatctgact cgcttcaatg
    16081 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    16141 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    16201 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    16261 agtacaaaac catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    16321 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    16381 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    16441 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    16501 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    16561 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    16621 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    16681 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    16741 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    16801 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    16861 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    16921 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa
    16981 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    17041 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    17101 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    17161 gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc
    17221 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    17281 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    17341 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    17401 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    17461 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    17521 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    17581 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    17641 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    17701 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    17761 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    17821 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    17881 aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt cattcgcatt
    17941 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    18001 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    18061 cctttgccgt tggcacctaa ataatcagtg atcgcctgag cgccattttc actggtcgca
    18121 gtgccgataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    18181 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    18241 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    18301 acaatgcgtt tcgtcaggcc agtaagcact ttgcccgggc cgacttcata gagatgttct
    18361 acgccttgcg ctgccatgta ttcaacagac ttcgtccact gaaccgggtt atacaactga
    18421 cgtaccagtg cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    18481 acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    18541 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagagcgcgt
    18601 tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ctgcttcttt atgaccggca
    18661 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct
    18721 tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcaccc
    18781 gttccttccg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca
    18841 tcagcgaaat caatcacacc agcgcaaacc agcgcggagt attcccccag gctgtgacca
    18901 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat
    18961 gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc cggcccctgc
    19021 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    19081 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    19141 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg
    19201 gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    19261 cgcccgtcgc gtacagcttc atccagagcg cacgggacag aggccgcaga ggtattaccg
    19321 tggcgatcca gcgtcaccac gacattgtcc atcgacatgc cgagtttttt cgctgttgca
    19381 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    19441 ttattcgccg ccagcgtctc atctacgatg tgcgccagtt ctgttaccgc aaccttgaag
    19501 acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacacg gtcagcatta
    19561 ggcagcgtca gcaactcacc atagctaccg tcggcatgca gatgggtgga gatgattccc
    19621 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    19681 ccacgatcgg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    19741 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg
    19801 caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccagcatgct ttgaatctga
    19861 caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc
    19921 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    19981 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    20041 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    20101 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    20161 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    20221 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    20281 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    20341 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    20401 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    20461 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    20521 agaaaggaac atcctgacaa caccttccat cgtctttaat gtgacatttc ctgtaaagcc
    20581 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    20641 ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcgagacc
    20701 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattaggaa tttccaccac
    20761 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtac tgtcacaatc
    20821 gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgcg gtaataccgt
    20881 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    20941 tagcgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    21001 cagtgccacg cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc
    21061 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    21121 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    21181 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    21241 catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    21301 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    21361 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt
    21421 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    21481 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    21541 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    21601 aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc
    21661 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    21721 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    21781 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    21841 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    21901 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    21961 cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcagga
    22021 gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    22081 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    22141 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    22201 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    22261 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    22321 ccacctcgcc ctggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    22381 cacccgaggt cgacgaaact ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    22441 tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt
    22501 cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg
    22561 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    22621 tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc
    22681 gtcacctgag cgaggcggag attgataatt acgtgcgtaa agagcatccc ctgcactgcg
    22741 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gaggggcgag
    22801 atcctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag
    22861 ggaaaaaccc gctgatggga tagttttctg cgccggatgc ggttacgtcg catccggcaa
    22921 tcggtgctgg cggcctgatg cggcgtaaac gccttatcaa ggccaaccgc ccattatcag
    22981 gtttatatgc ttgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc
    23041 atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa acttcaacgc
    23101 tgcggcgtgc aggaacagac gatttaatcc tgtgcctgct tcagtgagct gtctgtcaaa
    23161 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatact gtgtatgcac
    23221 acggatctga tgagtacgcc ctgttaccgg gctacaacgc accagggtgg caaaagcata
    23281 gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactca cacgcacgat
    23341 acgttcgccg ctttgcagaa tatttttcag caacggcgct tgaacgctct tcacatgcga
    23401 ctgccactga ccgcgcacca gcgccaggta atctttttgc atcccttttt cacgtaattg
    23461 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc
    23521 ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    23581 ttcgataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    23641 cagcaccagg atatggtcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    23701 cagatgtggc gaaaccgcct cttcttcccg ttcagcaacg cgaaccggtg ggatacgcac
    23761 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    23821 gcctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    23881 aaagttatcg atacgttgcc ccgcttcgtc agcgttgata gcaacaattt ttacggatgg
    23941 agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    24001 tctatgctta tatttagttt tgcaccttat tacttcacta cgtgatcact ttattgatgg
    24061 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    24121 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    24181 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    24241 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    24301 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    24361 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    24421 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    24481 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    24541 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    24601 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    24661 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc
    24721 acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc
    24781 atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg
    24841 ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta
    24901 gctatctggt tctgatgccg aacaacccgc gcgcgggtgg catttctcgt cgtatcgaag
    24961 gcgacgatcg taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg
    25021 gacttatcgt gcgcaccgct ggcgtcggca aatctgctga ggcgctgcaa tgggatttaa
    25081 gcttccgtct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc cctgctccat
    25141 tcctgattca tcaggagagc aacgtcatcg ttcgcgcatt ccgcgattac ttacgtcagg
    25201 atatcggcga aatccttatc gataacccga aagtgctcga actggcacgc cagcatatcg
    25261 ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc
    25321 tgttcagcca ctaccagatt gagtcacaga ttgagtccgc cttccagcgt gaagttcgtc
    25381 tgccatctgg tggttccatt gttatcgaca gcaccgaagc gttaaccgcc atcgacatca
    25441 actccgcacg cgcgacccgc ggcggcgata tcgaagaaac cgcgtttaac accaacctcg
    25501 aagctgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta
    25561 tcgacttcat cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg
    25621 aagcggtgcg tcaggaccgg gcgcgtattc aaatcagcca tatttctcgc tttggcctgc
    25681 tggaaatgtc ccgtcagcgt ctgagcccat cactgggtga atccagccat cacgtctgcc
    25741 cgcgctgctc cggtaccggt accgtgcgtg acaacgaatc gttgtcgctc tctattctgc
    25801 gtctgatcga agaagaagcg ctgaaagaga acactcagga agttcacgcc attgttcctg
    25861 tgccaatcgc gtcttacctg ctgaatgaaa aacgttctgc agtgaatgcg attgaaactc
    25921 gtcaggacgg cgtgcgctgt gtgattgtgc caaacgatca gatggaaacc ccgcactacc
    25981 atgtgctgcg cgtgcgtaaa ggggaagaaa ctccaacctt aagctacatg ctgccgaagc
    26041 tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtcctg
    26101 aacaacctgc gctggcaacc tttgccatgc cggatgtgcc gccagctcca accccagctg
    26161 aacctgccgc gcctgttgta gccccagcac ctaaagctgc aacggcaaca ccagcatctc
    26221 ctgcacaacc agggttgttg agccgcttct tcggcgcact gaaagcgctg ttcagcggtg
    26281 gtgaagaaac caaaccggcc gagcaaccag caccgaaagc agaagcgaaa ccggaacgtc
    26341 aacaggatcg tcgcaagcct cgtcagaaca accgccgtga ccgtaatgag cgccgcgaca
    26401 cccgtagtga acgtgctgaa ggcaacgata atcgcgaaga aaaccgtcgt aatcgtcgcc
    26461 aggcacagca gcagactgcc gagacgcgtg agagccgtca gcaggctgag gtaacggaaa
    26521 aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga acgtagccgc cgccgtaacg
    26581 atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag caatctgttc
    26641 aggaaaccga acaggaggaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc
    26701 tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg
    26761 ttgaagaaac tgtcgctgcc gaaccaattg ttcaggaagc gccagctcca cgcacagaac
    26821 tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca
    26881 atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc
    26941 tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccaatcgc
    27001 caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc
    27061 gctatccaat tgtacgtccg caagatgtac aggtcgaaga gcagcgcgaa caggaagaag
    27121 tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgtcgaa ccggttgtta
    27181 gcgcgccagt tgttgaagaa gtggctgaag ttgtagaagc ccccgttcag gttgccgaac
    27241 cgcaaccgga agtggctgaa acgacgcatc ctgaagtaat tgccgccgcg gtaactgaac
    27301 agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagttgca gaacacgcag
    27361 aaccggtggt tgaaccgcag gaagagacgg ctgatattga agaagttgcc gaaactgctg
    27421 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    27481 tcgcagcaga agttgaaacg gtagccgcgg ttgaaccaga gatcaccgtt gagcataacc
    27541 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    27601 acagtgactg gcagcgccct acttttgcct tcgaaggcaa aggtgccgca ggtggtcata
    27661 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    27721 ctcaagtaat caagccctgg taactgccag ggctttttta tttcaggggt agcatctttg
    27781 ctgtaatgac attagcgtta catgtactgt ataattatca tccacgcaaa gccgaaggat
    27841 atatttatgc cagcagccaa taacattcca gccaggcgcg aatctttaaa cttgcgcata
    27901 aaacccgcag aacgcgatct tattgaccgg gccgctaaag cccgaggaaa aaatcgcacc
    27961 gaatttgtcc ttgaggccgc cagagccgca gctgaagagg cgctcattga gcagagaatt
    28021 ttcaggatcg attctgatga ttatcagcta tttttgaatc gtctggatca gccccccgcg
    28081 cccaatgctg cattacgcaa aaccatgcaa acacccgcgc cgtgggagca aaaaaaatga
    28141 ttcaggcccc cgagccactc atggcaaggc atcaattcac ctctttttgc tcgggagtag
    28201 aaacgatgga taactggcta aagcagagag ccctaaaaaa tcagcttgcc ggcgcatcac
    28261 gtacatttgt tagctgtgat acgtatagca acgttttggc atattattcg cttgcttcca
    28321 gcgccgttga aacgtatgtc gcgacaggac gatttcgccg aaatatgcca gaaccgatcc
    28381 ctgttgtagt acttggtcgt ctggcaatag ataaatcatt acagggtcag ggcatcgatc
    28441 gtgcgatggt cagagatgcc ggtctacgag tactacaggc ggctgaagtc attggtattc
    28501 gcggaatgtt ggtccatgcg ttatcagacc aggcccgtga gttttatctg cgagtggggt
    28561 ttgaaccatc gcctgtcgat tctatgatac tgatggcaac attggctgat ttgcaggaat
    28621 gtcttaaata gttcgttatg tctcatgcaa aaaaatgccc gccccggcga tcccagagca
    28681 ggcagacaaa acatacccag tttcatgata tgttttaaag cgaaattatt tgttgagctg
    28741 gaagagcgac aatccctgca tatcggtaaa tgctttatac gacgcctgca atgccgtttg
    28801 ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg
    28861 ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc agcgactcca gttcgttcag
    28921 ctgcgtgcct aattccgcgc gaacagtcag cacattgttc agcgagtttt tcagtccgcg
    28981 gttggtttta tccagtgccg cagcggcggt ttctttatcc gcttcgctat ccgcgaccgg
    29041 cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct
    29101 accgtctggt tccgctaccg cgttgctggt aatactgtcg aaaattttgt cacccgtgtg
    29161 ccctatcacc atcgaacgcg aagcatcgac ctgttgttta atactttctg ctccgccttc
    29221 gtattttcca tccgcctcgc taaacggtgc ggattctgtt ttataaccgg caaaaatgta
    29281 gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctgaat
    29341 atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat
    29401 tttttcctga gcattctgga tagcggtggt gacctggcta agtacactct cttccagtga
    29461 cactttttga gtggcgaaag tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg
    29521 ggagagaaca acagcttgtg atgcagcaat gggatcatct gaagggttaa cgactcgctt
    29581 acccgtcgac atctgttcgc cgtacttcat ccattctgcc tgagaattgg tgataccacg
    29641 catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc ttagcgaatg
    29701 ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa
    29761 tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac
    29821 tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact ggttttcaac
    29881 gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc
    29941 gcaccgccca ccgttttact gttgctttgc agatcgagca gggcctgacc gttgcggttg
    30001 tcgctatcac ctgtatcttc tgcgctcgcc atggcgattt tcgcttcgtc ggtgattaat
    30061 acatccatgt tgacgatggc gtcacttacc ggtttcagcg tgaagctgtc gttaacggca
    30121 ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgct ggcatccggc
    30181 gtcaccgtaa aagtggtatt actggcaagg cgggtgacct gccactgatt attatcgaac
    30241 gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgttgc accgatcgca
    30301 acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagc aaagaaatct
    30361 tcaccggcat cgccgttagc atcaaatccg gctttgtgtt gggtgttgaa agcctcggca
    30421 aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat
    30481 gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct
    30541 gccgtcccat caacataagc gacagtcgta cgagaagggt cagcgctgga aggaactgcc
    30601 gccagttgcc gagccgtact tccctgaacc agtgagtaac cattggccat cgtgatgtta
    30661 taagtgccac catcctgaac gctgacttct acaccaacaa tctggtttaa ttcgctcacc
    30721 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    30781 cgggagattt gatcgttcag gctggcgatt tgtttagcgt aattgttgat ctgatcaacg
    30841 ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt
    30901 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccggatct
    30961 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    31021 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcggtc
    31081 aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgc atcatactca
    31141 cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta
    31201 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    31261 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg
    31321 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcgc
    31381 cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag gttttgctca
    31441 ccttgtcgct tatcgatttc atctgctgaa tcatgctggt gagtttgcgg gcatagtgag
    31501 gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgcggcgg
    31561 tcgtcacggc ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc gacaatgctt
    31621 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccattttcat
    31681 attcagtcgt ggtgatttca gtgactggcc ctttccagtt gccagaggct ttgacgccaa
    31741 acaggttata gctcggctcg ccgttttcac ggcggatttg ccgttgtccc cagccagatt
    31801 ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    31861 gggcgggcaa cgagagttgc gcgaggaatg ctttactgtc acccggcagc gaatcatcgt
    31921 agttacgtgg tacggccttt tgcaccagct gcgaaagcgt ctgattttga taacgcacca
    31981 cggtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
    32041 ctggcgtcat ctgtttaacc atcatctctg ccagccccag acctttgccc gccgtcatct
    32101 gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca
    32161 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
    32221 ccacctggcg ggccaccgga cggatatttg ccgccggatc ttcgcccgct ttcgccttaa
    32281 gttcgttgag cgattgtgca tcccaggccg cacttgccag tagtttgctg tcgctgatca
    32341 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcaaaataga
    32401 catcagatcc atcggcgtag cgcccagcgc attgagcgca cgcaccacgt tattgaggct
    32461 ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg
    32521 tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
    32581 gcgattaact gttactgaga gatttccctg cgctaccgcg cagctgtcga gggtcacttc
    32641 gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
    32701 aacattaacc tgcatattct ggatatcggc aaggaaacgg acctgggaac tgttgccact
    32761 cggtacgcgc acctgaatag tccgcgcatc caacgcggtg gcgctgccat atccacgcac
    32821 tcggttgatg gtgtcagcga tttgctgtgc catgctgaaa tcttcgtcgt taagttgcaa
    32881 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
    32941 ggtgatccgt ccaccgttca gttggttcac ctgaacactg ctaccgccag cggaggcccc
    33001 tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
    33061 aagcggtgtc atcaataacg taccgccacg cagacttttg gcatttccca tggaagaaac
    33121 caccacgtca atggtttgcc cctgacgtcc aaacggtgga agtgacgctg tcaccataac
    33181 cgcagcgacg ttttttagct gcatattagt gcccgtcgga acggtaattc ccagctgtga
    33241 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctga gttgtctggt caccagttcc
    33301 atccagcccc accaccaggc catagccaat cagtgagttt tgccttaccc cctgaacact
    33361 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa gaattaatgc
    33421 agagagaaat ttaatcacca cagcctcact tacattggag acaggttaag aaagaaacgc
    33481 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc
    33541 gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
    33601 acgccagaga agcgaataaa ttcggtaccc tgattaatgg cgatctgttt ttcacccacc
    33661 acatgcaggt tgccgttaac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
    33721 gtattactgg cattggcccc gccttttccg ttgaacgtgt taccaccgga ggcttcgaca
    33781 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
    33841 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
    33901 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
    33961 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
    34021 ggaccgggaa ccggttgtgc actggtcgcc ccctgtacca gcggcgtgga gggtatccag
    34081 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
    34141 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aatctgccac cggttatgcc
    34201 ttagagttgc gtcagttttt gcagcatctg atcggtggtg gataccgctt tactgttgat
    34261 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
    34321 cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt
    34381 cggtgcaccg gaggattgcg tttcggtgta gaggttttcg ccaatactct ccagtccggt
    34441 atcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
    34501 ttgtgttacg ctgaccacgc catcacgacc gatggtgata cttagcgcat tcgccggaat
    34561 ggtgatcgct ggctgcacct gaaaaccacc tgccgtcacc agctgcccgt tctgatccac
    34621 ctggaaagag ccgtcacggg tataggctga tgagccatct ggcaacatca cctggaaaaa
    34681 gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacagat tgccctggct
    34741 gtgtaagcgt tcagtggcga ccgggcgtac acccgtgccg atttgtaatc cggatggtaa
    34801 ggtggtttgt tcggaagact gagcacctgg ctggcgaatg gtttgataaa gcagatcttc
    34861 aaataccgcg cgctgacgct taaaaccgtt ggtactgacg tttgccaggt tgttggcaat
    34921 gacgtccata ttggtttgct gggcatccag gcccgttttg gcgatccata atgaacttat
    34981 cataaaatgt cctgtagtgg attagtgaca aagtgcatct gtcttcttgc cggatgcggc
    35041 gtaaacgcct tatccggcct acaagagctc gcgaaatcaa taaattgcga ttgccccata
    35101 gacccgataa acttgcgcat cgggcaactt atacttgtca tgtatccttt caattaactc
    35161 atcgacagca gttggttggc tcggcctgcg ttatcatcga cgctgctgat caccttcatc
    35221 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    35281 ttactgcctt ccagaacacc cgacatcaca cgcaaggttg gatctgcctg cagtaccggc
    35341 ccacgcgtgg tctgggtttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctggact
    35401 tcgctgcccg tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccgga
    35461 tcgcccggat tcagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc
    35521 ggcacagcaa ttggcccagc ctcgcctatc accggatgtc cctgaattgt cagttgcccg
    35581 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctaccgtc agcggtctgc
    35641 acggccagcc agccatcctg ctgcaacgca acgtccagcg ggcgcgaggt gtaatccatc
    35701 ttgccgggcg tcatgtctgc acccggcgtt gacgccgtta ccagcgtgcg cgtaggcagt
    35761 gaaagccctt ccaccggcac tgcgcgcaag gcgtttaact gcgcgcgaaa accgggcgtt
    35821 gaggcattgg ccagattact ggcggttacc gcctgttgat tcaacgtctg gctggctgct
    35881 cccatcgcgg tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt
    35941 aaccagcgtg ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt
    36001 acgctgagca acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccag
    36061 cgcaccgttg gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc
    36121 agaagattgc gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc
    36181 aaagttcgcc agtacaatct gccccagcag ttgggtttgt tcgttggaat agttgccgac
    36241 aaccgtaccg tcatcattga tttgataact caccagatcg cccggtttgt agccgttctg
    36301 ggtggttgcc acaatgttgt tagcgccggt attttgctgc atggagttga ggaagctcag
    36361 gctaaacgtg gcgggttctg caccgttaat tgcgccagtg gaaatattcg ctgttggatc
    36421 tgaggtcaga acgccattgg cattaaacac cagcgtcatt gcaggctcag ctgtacctgt
    36481 tggatcactg ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta
    36541 gacgctcatg tcatgagcat taccctgact atcgaaaaca gtcaccgaac cttttttgtt
    36601 atagctatcc gcattgctag catcaaatgc gttaacagag ggaagcggat cactggaatt
    36661 caggttgatc tgcatcgacg ccgtggtggt agttttcgct gccatcaggg tattcggaat
    36721 cgaaatattg gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta
    36781 acccgtcagc tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg
    36841 tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg
    36901 gctgatagca acgtccagac ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg
    36961 agtgatacca gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa
    37021 agaggccgtg cctgatttaa agccgtaggt ggcagagttg gcgatattgt tgccaataac
    37081 atcgaggttg gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga
    37141 ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta
    37201 agtgccgaga tccagcgtat taccgttgtt gccgcggatc acaccctgca ccagagcaaa
    37261 ctgcagcggc tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacgttgta
    37321 agaaccgttc ggcgcagtgg tgccgtcagt caacgtaccg tcccaggtga aactgtggac
    37381 tccggcggtc agttcaccaa tatcaatggt acggacaacc gcaccgtttt tatcggtgat
    37441 ggtggccgtc actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt
    37501 cacagcccct tcttcactgc cggttcccgc aagaacagtg gtgccgggga tcatcacgcc
    37561 gtgaccgatc aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga
    37621 tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg ctgatttgag ccaattgcga
    37681 cgtcagctcg ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccaa
    37741 caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactact
    37801 ggtggtgctg acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagtttt
    37861 ctcctttatt gaccgagcgt aagggttttc agcatcatgc ttttcaccgt gttgagcact
    37921 tcaacattgg cctgatagct gcgtgacgcc gacatggtgt taaccatctc tccgacgaca
    37981 tcaacgtttg gcattttcac gtagcccttt gcatccgcca gcggattacc cggttcataa
    38041 accagtttgt ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca
    38101 gcacctggcg cagcgttaac ctgaaatacc acctgttttg cccgatatgg ctgtccatcg
    38161 ggaccggtca cgctatcagc attcgccaga ttactggccg ccacgttcag gcgctgggac
    38221 tgagcagtta acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt
    38281 ccgctctgta aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc
    38341 tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacagtatta
    38401 ccatccagcg agggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc
    38461 gcctgcgccg gaatgtgttg cgttgaggtc atcgtcagtg caaccacgct tgttgcatcc
    38521 cgtccacgtt gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa
    38581 ccaggggtat cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga
    38641 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    38701 caggtatcaa aattcggcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    38761 aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg
    38821 ttgtagaaat ggctgggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc
    38881 gatactgttc agtccgttaa gtgcggcgag caatctcaca tcgcaattgc acaccttttt
    38941 tagcgcccag ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa
    39001 tctactaccg ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg
    39061 caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtgca
    39121 ggccacagga aattatgtgg ttgccgcaat gcccattgtg cggggaggaa agctggaagc
    39181 tggcaatgtc aaactgaaac gcggacggct ggacaccttg ccaccgcgta cggtgctgga
    39241 tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca
    39301 gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc
    39361 cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg ctgccgtcgc
    39421 acagaatgcg cgggtgcgca tggtatcggg acaagtggtc agcggcgttg ttgatgcaga
    39481 tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata
    39541 agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc
    39601 gcctctgaag cccgtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa
    39661 cacccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa gcgacgcgca
    39721 agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa
    39781 actagcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat
    39841 caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacgcgtctt gcagagatcc
    39901 tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac
    39961 atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct
    40021 ctctgctggc gacgctggat tacctcgaac agttacgcag gaaagaaccc aatgcagcaa
    40081 atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag caactacgcc
    40141 aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc
    40201 tggaaatgtt gaaaccgcat caggaaccga cgctatatgg agcgaacggt cagacctcaa
    40261 caacccatcg cggcagtaaa aagatttcga tctgaagatt atctccggcc tgcactgcag
    40321 gccggaatgc ctttttacac cgtccggcgg gcaaactctt taactttgaa gcccagtacc
    40381 gccagtgctg caaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagtaaa
    40441 cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg
    40501 ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccgggttgc
    40561 ggggtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc
    40621 gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccgataaa cgccaggttc
    40681 atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg
    40741 cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag
    40801 taggcaatta acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt accgtactgg
    40861 aacagcgaaa cggttaacgg acctgaaaga atgcccaacg caaccgcact cggcagcgcc
    40921 aacaggaaac aaagacgcag cccccagtcc atcaaacggt tgtattcatc atgattgcca
    40981 ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac gcccagtaca
    41041 ccggacggaa actccattaa gcggtcggcg taatacatcc aagacaccga accggaagca
    41101 agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg
    41161 atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa gttaatgcgc
    41221 ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag ctgcaggatg
    41281 ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca
    41341 aacagcgcga aaccaatcat gctgatatta agcagtgttg gagcaaacgc cggaatcgag
    41401 aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata
    41461 tagggaaagg ttatctttag tagctggctg gtcagggcaa atttgtcggc agtgtcagcg
    41521 aagcctggcg cggtcaccat gatcacccac ggtgcagcga gcatgccagc gaccgtcaca
    41581 accgccagcg cgagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct
    41641 tcaccctgct tgcttttata ttctgccaga atcggtacaa atgcctggga aaatgcccct
    41701 tcggcaaaga tacggcgcaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt
    41761 gccatccctg cgccaaagat tctggcgaca attgcgtcac gtgcgaagcc aagcacacgc
    41821 gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta
    41881 atccagaccc acgggcaaaa cgcctgcaaa gcaggcgctg aaagaggcga atagtctacc
    41941 tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg
    42001 ccagatcttg tcaacgatac gttgcgcccg cactgcctgt tcgccggcgg tttgcggaac
    42061 tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc
    42121 aagcgtactc tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg
    42181 ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc
    42241 acgctggctt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac cggccgaaaa
    42301 atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag
    42361 agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat aatcatccag
    42421 caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat ccattcttag
    42481 cgaggctgcg gtggcgagtt gcgtttttaa ctctccgtag agtggtgcga aacgacggtt
    42541 aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccaacc gttcagcatc
    42601 gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgcat tgagtaacgt
    42661 actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc
    42721 ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc
    42781 tttcgcgcgc gtaggcgacc aggctccttg taacatccag tcagacgctg ccgccagcac
    42841 cggtaaccac gctttttgcg caatgccgcc taatcccact acgccgatac gtaatttttt
    42901 cactttaatc tcccagatgg gcaagcaacg aatcaagacg ctgtttcagt tctgccactt
    42961 cgatttccag ggcttcgacg cgagcctgta aatcaccgtc aaccgcgttc gacatatcca
    43021 tcaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg ctttcacgtt
    43081 tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt gccagttgtt
    43141 ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc
    43201 gcagttcacc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttctg
    43261 cggcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga
    43321 cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga ttatccagct
    43381 gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt ttctgattac
    43441 aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt
    43501 ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc atgagattct
    43561 ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat
    43621 tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc
    43681 agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt
    43741 tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc
    43801 tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc acgaacaaca
    43861 ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag
    43921 aaagctgaac cttgtttatg aaattcgtta atcatcccta gcctggcctg ccagcctgat
    43981 ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct
    44041 gcgtaataat ccgccagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg
    44101 tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc
    44161 cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt
    44221 gaaaagtcca gaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa
    44281 agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat
    44341 gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag
    44401 gccaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc
    44461 tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc
    44521 atggtcggta ttgccctcgg cctacgccag tttattcagc aaggtctggg tattttcggc
    44581 ggagcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt
    44641 gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctactgtg gttttcatgc
    44701 ctgctttcgg gactcggcgg cacgttgttt gatcctccgc gttcggcgct ggtggtgaaa
    44761 ttaatccgtc cacagcagcg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc
    44821 ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc
    44881 tgcgccacag gggcagtttt gtttgtgctg tgtgcagcgt ttaatgcgtg gttgttaccg
    44941 gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc
    45001 gacaagcgtt ttgtcaccta tgtactgacg ctggcgggtt actacatgct ggctgtacaa
    45061 gtgatgctga tgctgccaat tatggtcaac gacgtggcgg gcgcgccctc tgccgttaaa
    45121 tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg
    45181 agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctattgat aatgtcatta
    45241 agcatgatgc cggtgggcat ggtcagcggt ctgcaacaac ttttcaccct gatttgtctg
    45301 ttttatatcg ggtcaatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg
    45361 gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc
    45421 gctattggtt acatcggtgg cggctggctg tttgacctgg gcaaatcggc gcaccagcca
    45481 gagcttccgt ggatgatgct gggcattatt ggcatcttca ctttccttgc gctgggttgg
    45541 cagtttagcc agaaacgcgc cgcgcgtcgt ttgctggaac gcgacgcctg atttgatact
    45601 cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag
    45661 ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa
    45721 aatatcccca tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg
    45781 gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc
    45841 atggacatcg ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt
    45901 tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg
    45961 ccgcgttttg ccaaatcggc atttgatgag ttttctactc ctgccgcgcg caaatatttc
    46021 gtcgacaaga aagaggctag cgcgggtaat tttgccgacc tgctggccca ctctgacggt
    46081 ctgattaaga atatcagcga tgatttacgt gcgctggaca aactgatcgt caaaccgaac
    46141 gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctcct gcgtaatctg
    46201 acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg
    46261 aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgcggca
    46321 gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgcgtt tatcgctggt
    46381 gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa
    46441 caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact
    46501 cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt
    46561 ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga caaacctgac
    46621 cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga
    46681 catgaactcc ctgttcggta gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc
    46741 cgtgtttgat aaagaaaaag gcgctatctt cctgaaagag atggaagtgg tcgatgcgac
    46801 ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt taaaccaggc
    46861 attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg
    46921 cgaagcaatg gcgaagaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat
    46981 tccgtttact gattaatcac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa
    47041 acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt
    47101 aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct
    47161 ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc
    47221 tatcgtaatg cccaatctgg ctccgcccgt gaccaccgtt gaggctgccg tggcttatcg
    47281 ccagcgtatt cttgacgccg tacctgccgg gcacgatttc accccgctga tgacctgtta
    47341 tttgacagat tcactagacc ctaatgagct tgagcgtgga tttaacgaag gcgtgttcac
    47401 cgctgccaaa ctttacccgg caaacgcaac cactaactcc agtcacggcg tgacgtcagt
    47461 tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgt tactggtgca
    47521 tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttattgaaag
    47581 cgtgatggaa ccgctacgcc agcgtctgac tgcgctgaaa gtcgtttttg agcacatcac
    47641 caccaaagac gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac
    47701 tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct
    47761 gtattgtcta cccatcctca aacgcaatat ccaccaacag gcattgcgtg aactggtcgc
    47821 cagcggtttt aatcgcgtat tcctcggtac ggattctgcg ccgcatgcac gtcatcgcaa
    47881 agagagcagc tgcggctgcg cgggctgctt caacgcccca accgcgctgg gcagttacgc
    47941 taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg
    48001 cccacagttc tatggcttgc cggtcaacga cacattcatc gaactggtcc gcaaagagca
    48061 acaggttgct gaaagcatcg cattgactga tgacacgctg gtgccattcc tcgccgggga
    48121 aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt
    48181 ttaactgtat aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca
    48241 tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc
    48301 gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag
    48361 cgaataattt atcggttatt ggcgcgacaa aagaagataa acagcgcatt agcgaaattc
    48421 tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt
    48481 ttgtttttgc cgggtcgccc cggctttttt attttacttg cgaattgtga gtacaaaatt
    48541 tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga catgctgttt
    48601 tattgttcat ataaatctta aaagcaaaaa tttatctgtt ccccccttta aaaattcggt
    48661 gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga
    48721 ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt
    48781 cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg
    48841 ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga agttggtcag
    48901 acgctctggt taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc
    48961 gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta acatgacatg
    49021 accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca
    49081 ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc
    49141 atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgtgccggt
    49201 ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag ccaccacggc
    49261 gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc gctggttctc
    49321 cgcgcgcaaa cgctgtggga tgaactctcc cgccacaacg aagaagatcc catttttgta
    49381 cgctctggcg tcattaacct tggcccggct gattccacat ttctcgccaa cgtcgcccac
    49441 agcgccgaac aatggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc
    49501 tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggcttt
    49561 ttacgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg
    49621 cagctgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa
    49681 acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa
    49741 gacctgctcc cggagttgcc tgtccagccc gtacgcaaag tatttgcctg gtatcaggcc
    49801 gatgggcgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact tcccaatggc
    49861 gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga
    49921 ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt
    49981 tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc
    50041 gccgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc
    50101 cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt
    50161 ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca
    50221 ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac
    50281 aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc gagcatctga
    50341 tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat gtgtattatt
    50401 tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca tttagcgtga
    50461 aaaattgaat gagattattc ctgcttcaac agcactcacc agacatctc
//
LOCUS       DAZGTJ010000027        49958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000027 DAZGTJ010000000
VERSION     DAZGTJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.027"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            125..199
                     /locus_tag="ACHK8E_001963"
     tRNA            125..199
                     /locus_tag="ACHK8E_001963"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:157..159,aa:Gln,seq:ctg)
     gene            237..311
                     /locus_tag="ACHK8E_001964"
     tRNA            237..311
                     /locus_tag="ACHK8E_001964"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:269..271,aa:Gln,seq:ctg)
     gene            complement(465..1640)
                     /gene="ubiF"
                     /locus_tag="ACHK8E_001965"
     CDS             complement(465..1640)
                     /gene="ubiF"
                     /locus_tag="ACHK8E_001965"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP0344238.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            1786..3210
                     /gene="miaB"
                     /locus_tag="ACHK8E_001966"
     CDS             1786..3210
                     /gene="miaB"
                     /locus_tag="ACHK8E_001966"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP0344239.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3363..4403
                     /gene="ybeZ"
                     /locus_tag="ACHK8E_001967"
     CDS             3363..4403
                     /gene="ybeZ"
                     /locus_tag="ACHK8E_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP0344240.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4400..4867
                     /gene="ybeY"
                     /locus_tag="ACHK8E_001968"
     CDS             4400..4867
                     /gene="ybeY"
                     /locus_tag="ACHK8E_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP0344241.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4957..5835
                     /gene="corC"
                     /locus_tag="ACHK8E_001969"
     CDS             4957..5835
                     /gene="corC"
                     /locus_tag="ACHK8E_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP0344242.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5860..7398
                     /gene="lnt"
                     /locus_tag="ACHK8E_001970"
     CDS             5860..7398
                     /gene="lnt"
                     /locus_tag="ACHK8E_001970"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP0344243.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(7476..9353)
                     /locus_tag="ACHK8E_001971"
     CDS             complement(7476..9353)
                     /locus_tag="ACHK8E_001971"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP0344244.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            9808..10716
                     /gene="gltI"
                     /locus_tag="ACHK8E_001972"
     CDS             9808..10716
                     /gene="gltI"
                     /locus_tag="ACHK8E_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP0344245.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10886..11626
                     /gene="gltJ"
                     /locus_tag="ACHK8E_001973"
     CDS             10886..11626
                     /gene="gltJ"
                     /locus_tag="ACHK8E_001973"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP0344246.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11626..12300
                     /gene="gltK"
                     /locus_tag="ACHK8E_001974"
     CDS             11626..12300
                     /gene="gltK"
                     /locus_tag="ACHK8E_001974"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP0344247.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12300..13025
                     /gene="gltL"
                     /locus_tag="ACHK8E_001975"
     CDS             12300..13025
                     /gene="gltL"
                     /locus_tag="ACHK8E_001975"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP0344248.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13143..14078
                     /gene="rihA"
                     /locus_tag="ACHK8E_001976"
     CDS             13143..14078
                     /gene="rihA"
                     /locus_tag="ACHK8E_001976"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP0344249.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(14123..14605)
                     /gene="ybeL"
                     /locus_tag="ACHK8E_001977"
     CDS             complement(14123..14605)
                     /gene="ybeL"
                     /locus_tag="ACHK8E_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP0344250.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            14840..17422
                     /gene="leuS"
                     /locus_tag="ACHK8E_001978"
     CDS             14840..17422
                     /gene="leuS"
                     /locus_tag="ACHK8E_001978"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP0344251.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17437..18018
                     /gene="lptE"
                     /locus_tag="ACHK8E_001979"
     CDS             17437..18018
                     /gene="lptE"
                     /locus_tag="ACHK8E_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP0344252.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            18018..19049
                     /gene="holA"
                     /locus_tag="ACHK8E_001980"
     CDS             18018..19049
                     /gene="holA"
                     /locus_tag="ACHK8E_001980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP0344253.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            19051..19692
                     /gene="nadD"
                     /locus_tag="ACHK8E_001981"
     CDS             19051..19692
                     /gene="nadD"
                     /locus_tag="ACHK8E_001981"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP0344254.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19716..20327
                     /gene="cobC"
                     /locus_tag="ACHK8E_001982"
     CDS             19716..20327
                     /gene="cobC"
                     /locus_tag="ACHK8E_001982"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP0344255.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            20587..20904
                     /gene="rsfS"
                     /locus_tag="ACHK8E_001983"
     CDS             20587..20904
                     /gene="rsfS"
                     /locus_tag="ACHK8E_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP0344256.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20908..21375
                     /gene="rlmH"
                     /locus_tag="ACHK8E_001984"
     CDS             20908..21375
                     /gene="rlmH"
                     /locus_tag="ACHK8E_001984"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP0344257.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21406..23307
                     /gene="mrdA"
                     /locus_tag="ACHK8E_001985"
     CDS             21406..23307
                     /gene="mrdA"
                     /locus_tag="ACHK8E_001985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP0344258.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23310..24422
                     /gene="mrdB"
                     /locus_tag="ACHK8E_001986"
                     /gene_synonym="rodA"
     CDS             23310..24422
                     /gene="mrdB"
                     /locus_tag="ACHK8E_001986"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP0344259.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24433..25521
                     /gene="rlpA"
                     /locus_tag="ACHK8E_001987"
     CDS             24433..25521
                     /gene="rlpA"
                     /locus_tag="ACHK8E_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP0344260.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25661..26872
                     /gene="dacA"
                     /locus_tag="ACHK8E_001988"
     CDS             25661..26872
                     /gene="dacA"
                     /locus_tag="ACHK8E_001988"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP0344261.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26982..27245
                     /gene="ybeD"
                     /locus_tag="ACHK8E_001989"
     CDS             26982..27245
                     /gene="ybeD"
                     /locus_tag="ACHK8E_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP0344262.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27346..27987
                     /gene="lipB"
                     /locus_tag="ACHK8E_001990"
     CDS             27346..27987
                     /gene="lipB"
                     /locus_tag="ACHK8E_001990"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP0344263.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            28247..28770
                     /locus_tag="ACHK8E_001991"
                     /pseudo
     CDS             28247..28770
                     /locus_tag="ACHK8E_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            29011..29976
                     /gene="lipA"
                     /locus_tag="ACHK8E_001992"
     CDS             29011..29976
                     /gene="lipA"
                     /locus_tag="ACHK8E_001992"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP0344264.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(30077..30280)
                     /gene="tatE"
                     /locus_tag="ACHK8E_001993"
     CDS             complement(30077..30280)
                     /gene="tatE"
                     /locus_tag="ACHK8E_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP0344265.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(30409..31197)
                     /locus_tag="ACHK8E_001994"
     CDS             complement(30409..31197)
                     /locus_tag="ACHK8E_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP0344266.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            31290..31673
                     /gene="crcB"
                     /locus_tag="ACHK8E_001995"
     CDS             31290..31673
                     /gene="crcB"
                     /locus_tag="ACHK8E_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0344267.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(31727..31936)
                     /gene="cspE"
                     /locus_tag="ACHK8E_001996"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(31727..31936)
                     /gene="cspE"
                     /locus_tag="ACHK8E_001996"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0344268.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(32111..32671)
                     /gene="pagP"
                     /locus_tag="ACHK8E_001997"
     CDS             complement(32111..32671)
                     /gene="pagP"
                     /locus_tag="ACHK8E_001997"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP0344269.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            33260..34645
                     /gene="dcuC"
                     /locus_tag="ACHK8E_001998"
     CDS             33260..34645
                     /gene="dcuC"
                     /locus_tag="ACHK8E_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0344270.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(34686..35366)
                     /gene="dpiA"
                     /locus_tag="ACHK8E_001999"
                     /gene_synonym="citB"
     CDS             complement(34686..35366)
                     /gene="dpiA"
                     /locus_tag="ACHK8E_001999"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP0344271.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(35335..36993)
                     /gene="dpiB"
                     /locus_tag="ACHK8E_002000"
     CDS             complement(35335..36993)
                     /gene="dpiB"
                     /locus_tag="ACHK8E_002000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP0344272.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            37373..38431
                     /gene="citC"
                     /locus_tag="ACHK8E_002001"
     CDS             37373..38431
                     /gene="citC"
                     /locus_tag="ACHK8E_002001"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP0344273.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            38446..38742
                     /gene="citD"
                     /locus_tag="ACHK8E_002002"
     CDS             38446..38742
                     /gene="citD"
                     /locus_tag="ACHK8E_002002"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP0344274.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            38739..39647
                     /gene="citE"
                     /locus_tag="ACHK8E_002003"
     CDS             38739..39647
                     /gene="citE"
                     /locus_tag="ACHK8E_002003"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP0344275.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            39658..41190
                     /gene="citF"
                     /locus_tag="ACHK8E_002004"
     CDS             39658..41190
                     /gene="citF"
                     /locus_tag="ACHK8E_002004"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP0344276.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            41194..41745
                     /gene="citX"
                     /locus_tag="ACHK8E_002005"
     CDS             41194..41745
                     /gene="citX"
                     /locus_tag="ACHK8E_002005"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP0344277.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            41720..42598
                     /gene="citG"
                     /locus_tag="ACHK8E_002006"
     CDS             41720..42598
                     /gene="citG"
                     /locus_tag="ACHK8E_002006"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP0344278.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            42649..44112
                     /gene="citT"
                     /locus_tag="ACHK8E_002007"
     CDS             42649..44112
                     /gene="citT"
                     /locus_tag="ACHK8E_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP0344279.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            44226..45032
                     /gene="rna"
                     /locus_tag="ACHK8E_002008"
     CDS             44226..45032
                     /gene="rna"
                     /locus_tag="ACHK8E_002008"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP0344280.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            45262..45672
                     /gene="rnk"
                     /locus_tag="ACHK8E_002009"
     CDS             45262..45672
                     /gene="rnk"
                     /locus_tag="ACHK8E_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP0344281.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            45815..45904
                     /gene="yldA"
                     /locus_tag="ACHK8E_002010"
     CDS             45815..45904
                     /gene="yldA"
                     /locus_tag="ACHK8E_002010"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP0344282.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            46029..46457
                     /gene="uspG"
                     /locus_tag="ACHK8E_002011"
     CDS             46029..46457
                     /gene="uspG"
                     /locus_tag="ACHK8E_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP0344283.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(46578..48143)
                     /gene="ahpF"
                     /locus_tag="ACHK8E_002012"
     CDS             complement(46578..48143)
                     /gene="ahpF"
                     /locus_tag="ACHK8E_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP0344284.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(48314..48877)
                     /gene="ahpC"
                     /locus_tag="ACHK8E_002013"
     CDS             complement(48314..48877)
                     /gene="ahpC"
                     /locus_tag="ACHK8E_002013"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP0344285.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            49249..>49958
                     /gene="dsbG"
                     /locus_tag="ACHK8E_002014"
     CDS             49249..>49958
                     /gene="dsbG"
                     /locus_tag="ACHK8E_002014"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP0344286.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLP"
BASE COUNT        12206 a        12978 c        12500 g        12274 t
ORIGIN      
        1 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
       61 cccgtcgtag ccaccaaatt ctgaatgtgt cgaatatgtt cggcaaattc aaaaaccaat
      121 ttgttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg
      181 aatcctcgta ccccagccaa tttattcaag acgcttacct tgtaagtgca cccagttggg
      241 gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg
      301 taccccagcc acattaaaaa agctcgcctc ggcgagcttt ttgcttttct gcgtatattc
      361 aatgccgaat gcgatgttga ctcgtcttat ccttcaaagt cggatgcgac gctgccgcgt
      421 cttatccgac ctacggttgg catgcatcca gcaatgttgt gaggctacaa ccctaacgca
      481 tatttcagcg ccttacgttt caacacgcca gcacgctccg ccgccatcaa accaagatta
      541 cgcacaaaac gcagcggtgg cagattattg ctgaatccgg cataaaacag atccatacca
      601 ctttgcatca taaagttatc cgccatacgc cgcatctggt aacgcttgag cacaggataa
      661 ctggcccacg cttcgccgta gctgcgggcg ttcaccagaa cgtcaatcag ggcatcgaca
      721 tcacgataac caagattcac cccctgcccc gccagcggat ggatggtatg cgcggcatcg
      781 cccaccagag caagccctgg ttgcacatac tgcaacgcat ggcgacgtat cagcggaaac
      841 gcaccagcgg caagcggtgt cacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc
      901 gcctgtagct gcgccatatt catattctgc aactggcgaa tgcgcgccgg agagtcatac
      961 cacaccagcg atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggggta
     1021 aattgctgcc aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatacac
     1081 gactgcgcat actgccaggc atgaacgcca attcccgcca tctgtcgcac ctgcgaattt
     1141 gcgccgtcgg caccaatcac cagcttcgcg aggatcgttt caccgccctt cagttccagc
     1201 tcctgaagat cattatggcg atgcagtgca atcagcgagg ttggcacacg taacgttact
     1261 ttcggatgcg cttccagcgc ctgccacaac gcctgttgca ggacagtgtt ttccaccatg
     1321 tagccaagca acggtagctt aagttcagcg gcgtcaaaca ccacatgcgc cgtttcccac
     1381 tcccacgttt ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc
     1441 cctaaccctt taagcaatga taccgaagcc gcactaatcg ccgagatccg cacgtccggc
     1501 tggctgtcag cgacaaacgg tgcaggttgg gcatgttcga taaccattac cgaaaatccg
     1561 tgctgagcca gtcccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt
     1621 tccgttggtt gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg
     1681 atttttaccc ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca
     1741 ctatgcgccc tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc
     1801 catattaaaa cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg
     1861 ctggatgcca cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctgctg
     1921 ctgaacacct gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc
     1981 tggaaactgt taaaagagaa gaatccagac ctgattatcg gcgtcggcgg ctgcgtggca
     2041 tcgcaagaag gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg
     2101 caaacgctgc accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt
     2161 gtagatatca gcttcccgga aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa
     2221 gggccgaccg cgtttgtctc catcatggaa ggctgcaata aatattgcac ctactgcgta
     2281 gtgccttaca cccgtggtga agaggtaagc cgtccgtccg acgatattct gtttgagatt
     2341 gcccagcttg ctgctcaggg cgtgcgtgaa gttaacctgc tcggccagaa cgtgaacgcc
     2401 tggcgcggtg agaactacga cggcaccacc ggatcgtttg ccgatctgct gcgtctggtt
     2461 gcggcgatcg acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc
     2521 gacgatatca tcgaagtgta ccgcgacacg ccggagctgg tgagcttcct gcatctgccg
     2581 gtacagagcg gttccgatcg cattctgaac ctgatgggac gtacccatac agcgctggag
     2641 tacaaagcga tcatccgtaa gctgcgtgcg gcgcgtccgg atattcagat cagttctgac
     2701 ttcatcgttg gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt
     2761 gccgacgtca atttcgacat gagctacagc tttatcttct ccgctcgtcc aggtacacca
     2821 gccgccgata tggttgatga tgttccggaa gaagagaaga agcagcgcct gtatattcta
     2881 caagaacgta tcaatcagca agcaatggca tggagccgcc ggatgctcgg taccacccag
     2941 cgtattctgg tagaaggtac atcgcgtaag agcatcatgg agctttccgg ccgtactgaa
     3001 aataaccgcg tcgtcaactt cgaaggtacg ccggatatga tcggtaaatt cgtcgatgta
     3061 gaaattaccg acgtctaccc gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa
     3121 atgggtctgc gcgtggcaga aacaccggaa tcggtgattg cccgtacccg caaagaaaac
     3181 gaccttggcg tgggttatta tcagccgtaa ttctcaggcc cgccgttccg gtgggccttg
     3241 ttttttctct ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga
     3301 ataatttcct tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg
     3361 gtttgaacat agacactcgc gaaatcaccc tggagccagc agacaatgcg cgtctgttga
     3421 gcctgtgcgg cccgtttgat gacaacatca aacaactcga acgccgtctc ggcatcgaaa
     3481 tcaatcgccg cgataaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg
     3541 cagacattct gcgtagcctg tatgtcgata ctgccccgat gcgcggtcag attcaggata
     3601 tcgaaccgga acagatccac cttgcgatta aagaagcgcg ggtactggag caaagcgcgg
     3661 agagcgtgcc ggagtacggc aaagcggtca atatcaaaac caaacgcggc gtaattaagc
     3721 cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg
     3781 gcgttggccc ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc
     3841 tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc ggcagtagaa gccggtgaga
     3901 aactgggctt cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccgctgt
     3961 acgacgcgct gtttgaaatg ctgggctttg agaaagtcga gaaactgatt gagcgcaacg
     4021 ttattgaagt cgcaccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca
     4081 ttcttgatga gagccagaac accaccatcg aacagatgaa gatgttcctg acccgtatcg
     4141 gttttaactc aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata
     4201 ccaagtctgg tttgcgtcat gccatcgaag tactggctga tgtcgaagag atcagcttta
     4261 acttcttcca cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc gttaacgcct
     4321 atgaagcctg ggaagaagcc gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc
     4381 gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga
     4441 taattccggg ttaccggaag agagccagtt tcagacatgg ctgaatgcgg tgatcccgca
     4501 gtttcaggaa gaatcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct
     4561 gaatctgacc tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt
     4621 accgcctggc atggaaatgt cgctactggg cgatctggtt atctgccgtc aggtggttga
     4681 gaaggaagct caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca
     4741 cggcagtctg catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga
     4801 agccatcgaa acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa
     4861 agaataattt gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc
     4921 gttttgatta actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt
     4981 agtgacacga taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac
     5041 ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac
     5101 cttatcgacg aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc
     5161 gttcgcgaca tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg
     5221 gacgaatgcc ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa
     5281 gacaaagatc acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc
     5341 gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt
     5401 aagcgcgtag accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtc
     5461 attgacgaat tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactgatt
     5521 gttggtgaga ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt
     5581 cgtcatacct ggaccgtgcg cgcactggct tctattgaag acttcaacga agcgttcggc
     5641 acccacttta gcgatgaaga ggttgacact atcggtggtc tggtgatgca ggcatttggg
     5701 catcttccgg cgcgtggcga aaccatcgac atcgacggtt accagttcaa agtggcgatg
     5761 gccgacagtc ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagccg
     5821 aagctggatg aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt
     5881 gaacgccagc gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc
     5941 ttctctcctt acgacgtctg gcctgcggcg attatttcgc tgatggggct tcaggcgttg
     6001 acctttaacc gccgtccact ccagtctgcc gctattggct tttgctgggg atttggcctc
     6061 tttggcagcg gtattaactg ggtctatgtc agcatcgcga cctttggcgg aatgcctggc
     6121 ccggttaaca tcttcctggt ggtactgctg gcggcgtatt tgtcgctgta taccggactg
     6181 tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc
     6241 gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt gggtactaac aggcttcccg
     6301 tggttacagt tcggctatag ccagattgat ggcccgttaa aagggctggc accgataatg
     6361 ggcgtggaag ccattaactt cctgctgatg atggttagcg gcctgctggc actggcgttg
     6421 gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct tcccttcccg
     6481 ctgcgttaca tccagtggtt taccccgcaa ccggaaaaaa ctattcaggt ttcgatggtt
     6541 cagggcgata ttccgcaatc gctgaaatgg gacggagacc agctacttaa tacgctgaag
     6601 atatactaca acgcaacggc accgctgatg ggcaaatcat cgttgattat ctggccggag
     6661 tcggcgataa ccgatctgga aattaatcag caaccgttcc tcaaagctct ggacggtgag
     6721 ttgcgcgata aagggagctc gctggtcacc gggattgtcg acgcgcgtct caataaacag
     6781 aaccgctacg atacctacaa caccatcatc acactgggta aaggcgcacc gtacagctat
     6841 gaatcagccg atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccactg
     6901 gagtcgatac tgcgtccgtt agcaccgttc tttgatttgc cgatgtcttc attcagccgc
     6961 ggaccatata ttcagccacc gctgtcgttg aatggcattc aacttactgc ggccatttgc
     7021 tacgaaatca ttctcggcga gcaagtgcgc gataacttcc gcccggatac cgactatctg
     7081 ctgactatct ccaacgatgc gtggtttggt aagtctattg gtccatggca gcacttccag
     7141 atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc
     7201 attacggcgg tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc
     7261 gaggtgttaa ccactaacgt gacgccgact accggactca caccgtacgc acgtaccggc
     7321 aactggccgc tgtgggtgct gacggcactg tttggttttg cagctgtgct gatgagtctg
     7381 cgagcacgta aacattaatg tgcctgatgt atccggcatg aacaaaacat actttctcat
     7441 tgagttgtgg ggcatgatta tcatgccctt ttttcttacg gttcagggat acaagccaca
     7501 tcgccatatt ggggatgccg aataattgcc tgcggatcaa gcgagcgcgt cctgtccatt
     7561 tggattaacc tgttcttcgc cataacaaat atctttctac catccgctga aaaacgaagg
     7621 tgattaacgt agctggcagg ataaacgcca ataatctgcc cggcattaat attgagcagg
     7681 atgacataac cttgcaggcc atatgacacc aacgctaaat attgaccgtc cgggctaata
     7741 ccctcaacgg aataaacgct cctgctacac cattgcgtca taatgctggc ttgttgatcc
     7801 cctacgctta tacgtgaaat cccctggcta tcggcaaaaa taatctcgcc attttttggt
     7861 cggaatccca tcgcagaaac gataccacgc gcatcacctg tgggaaagtc ttctttctga
     7921 tgcgtgatgg tattgatgcg tacaatgtcg cctctgacca taccgggtat caacaatgtt
     7981 ttttcatccg gggaaagtgc cataacccag gcgcctggtt taccgctcgt ccccgtcccg
     8041 tcgtatttct ccagcgggat atccgtcaca tgctgataag tcaccagatc aatggctgac
     8101 acactattac tggtgcgatt catcacccag gcttttgtgc catcacgcga gaggataagg
     8161 ctgtcaggat gggcgccggt ggtaatcgac tgaataatct cccccgtcgc cacgttaatg
     8221 accgatactg cattcgcttc aaatgaagtc gcataaatca acttttcatc caggcttaat
     8281 gccagcgatc tgacgccctg atctttacag ccaccacaat gtttgactga cttttctttc
     8341 gctatgggcg ctgtaaaggt atgcaggatt ttcttttgtc cgaggtcgaa aacaacaatg
     8401 ctgttggtgt tttcaattgc ggcatacaac gtattaccat cccgactgat cgccatatca
     8461 tggatatcgg caatctcagc gagtaaatca ccactggcct gctcacgact taccggagcg
     8521 gataatgttt ccctgagggc gttgcgctcc tccactaact gctgcttttt ttgtcgtcgc
     8581 tcttgttcaa tctcctgcgg ataaaacgct tcacgcaggc tttgcctgac ctgtaaaact
     8641 gattcatcaa tctgctgctg cacaaaccag atagtcccgg tcagaagaag agtgaccgca
     8701 acaataatcc cgccttccgt aacgagccga ttttgcccaa ctaaacgcgc actcagccaa
     8761 cccaacgcgc cccccgcaat caggccgcca atgtggcaag cgttatctat tcctgcttgc
     8821 cgggcaccat acaacagtgt cagcgccacc atacccaaca gagaaaatac tcgccgctgg
     8881 tttttatggt gtgtacccgc acctgaagaa agctgtgttg ctatcgcagc gcccgctatt
     8941 cccataattg ccccggatgc cccaacgctg atgactatct gctcactttc gcgtaacgca
     9001 taacaggcgc tgatcagccc accaaagatg ccggagaata accagataat caggagtcgc
     9061 catttcccca gaatacgttc caccgctccc cccaccacca ggagtgcaaa catattcatc
     9121 agcaaatgag aaacactgct gtgcagaaag acactgctca ataagcgcca ggattcaccc
     9181 gacaacgtga gagaagcaac gttagcaccg agatggataa gtgcggattg atcgacttct
     9241 tcataactca tccattgtgc gaggtaacaa aatatcccaa cacagagcgc aaaaaggata
     9301 agggttattg cgggtaattg aacatttaac ggcttaaccg aagatgcaga cataaggtga
     9361 ttcttccttg aaaagacagg taaaaagcat ttttcccgtg tgcagttatc atcccggcaa
     9421 acgtcccatg ccgaaaagat aaccctgtcc gaaatatgac tatttcagcc taacattaca
     9481 atcgtaatta attcacctat aagtattttt tggcatgtaa gttgctttat ttactgtgta
     9541 agcaatgaaa tcagccgcga cgcaaccggg ttgcaccacc acaaatcagc ggtagcacca
     9601 aaaaggtgcg atttaagaat tttgcttcgg aatggtgcgg cgcatctcgc aaaaacaaac
     9661 aataaaacag caatatcttt acatttagcc aaactaaatg ttacctaaca agcacaacac
     9721 tgcacaataa agttgcagat gataacaaca caaacactca caacgggtat ccatgcgttc
     9781 ttaacgcaga agataaagga gttggatatg caattacgta aacctgccac agcaatcctg
     9841 gccctggcgc tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt
     9901 acgctggaca aaatcgccaa aaacggcgtg attgtcgtcg gtcaccgtga atcttcagta
     9961 cctttctctt attacgacaa tcagcaaaaa gtggtgggct actcgcagga ttactccaac
    10021 gccattgttg aagcagtgaa aaagaaactc aacaaaccag acttacaggt gaaattgatt
    10081 ccgatcacct cacaaaaccg tattccactg ctgcaaaacg gcactttcga ttttgaatgc
    10141 ggttctacca ccaacaatgc cgaacgccaa aaacaggcgg ctttctctga cactattttc
    10201 gtggtcggta cgcgcctgct gaccaaaaag ggcggcgata tcaaagattt tgccgacctg
    10261 aaaggcaaag ccgtagtcgt cacttccggc accacctctg aagttttgct caacaaactg
    10321 aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg tgactctttc
    10381 cgtaccctgg aaagcggtcg tgccgttgcc tttatgatgg atgatgctct gctggccggt
    10441 gaacgtgcga aagcgaagaa accagacaac tgggaaatcg tcggcaagcc gcagtctcag
    10501 gaagcctatg gttgcatgtt gcgtaaagac gatccgcagt tcaaaaagct gatggatgat
    10561 accatcgctc aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa gtggttcaaa
    10621 aatccaattc cgccgaaaaa cctgaacatg aatttcgaac tgtcagacga aatgaaagca
    10681 ctgttcaaag aaccgaatga caaggcactg aactaattac aagaaccagg ggcggaaatt
    10741 ccagccctct cgattgttac gtagcacgga cagactatac gcctggtggt cgttccccat
    10801 cgggcctgaa aaccgcaata cgctgggtaa caatcttcga gggtagcagt taacgctgct
    10861 accctctttt ttctggagta gatttatgtc tatagactgg aactggggta tttttttaca
    10921 acaagccccg ttcggcaaca ccacctatct tggttggatc tggagtggtt ttcaggtgac
    10981 gatcgctttg tcgatctgcg cctggattat cgctttcctc gtcggttcat ttttcggcat
    11041 tttacgtacc gttccaaacc gttttctctc cggtctgggt acgttgtatg tcgaactgtt
    11101 ccgcaacgtg ccgttgattg tgcaattctt tacctggtac ctggtgatcc cggagctgct
    11161 gccagagaaa atcggcatgt ggtttaaggc agagctggat cccaatattc agtttttcct
    11221 ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc
    11281 cgctattcag tcgctgccgc gtgggcaaaa aaatgccgcg ctggcgatgg gtctgacgct
    11341 accgcaggct taccgctacg tactgctgcc taatgcttat cgcgttatcg tcccgccgat
    11401 gacctcagag atgatgaacc tggtgaaaaa ctccgccatc gcctcgacta tcggtctggt
    11461 ggatatggcg gcgcaggcgg gtaaattgct cgattactcg gcccacgcct gggagtcgtt
    11521 taccgccatc acgctggcgt atgtattgat taacgctttt atcatgctgg tgatgacgct
    11581 ggttgaacgt aaagttcgtc tgcccggcaa catggggggc aaataatgta cgagtttgac
    11641 tggagttcca ttgttccttc cctgccatat ctgctcgacg ggctggtgat cacgctgaaa
    11701 atcaccgtca cggcagtggt gattggtatt ttgtggggca ccatgctggc ggtgatgcgc
    11761 ttgtccagct ttgcgcctgt tgcgtggttt gccaaagcct atgttaacgt attccgctcg
    11821 attcctttag tcatggtttt gctgtggttt tacctgattg tgccgggatt tctgcaaaac
    11881 gtgctgggat tatcgccaaa aaatgatatc cgtttaattt cggcaatggt ggcgttttcg
    11941 atgtttgaag cggcctacta ttcagagatt atccgtgccg gtattcaaag tatttcccgt
    12001 ggtcagtcga gcgccgcgct ggcgttgggg atgactcact ggcagtcgat gaaactgatt
    12061 attctgccgc aggctttccg cgcgatggtg ccgctgctgc tcactcaggg catcgtactg
    12121 ttccaggata cctcgctggt gtatgtgtta tctctggccg atttcttccg taccgcttca
    12181 accattggtg agcgtgatgg tacgcaggtt gagatgatcc tgtttgccgg atttgtttat
    12241 ttcgttatta gccttagcgc gtcgttgttg gtcagctact tgaaaagaag gacagcataa
    12301 tgattaccct gaaaaatgtt tcaaaatggt atggtcactt tcaggtgctg accgactgct
    12361 caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat
    12421 caacgctgat taaaaccgtc aacggcctcg aaccggtgca gcaaggtgaa atcaccgtcg
    12481 atggcatcgt ggttaacgac aagaaaaccg atctggcaaa gctgcgttcc cgcgtcggga
    12541 tggtattcca gcatttcgag ctgttccctc atctgtcgat tatcgaaaac ctgaccctgg
    12601 cgcaggtgaa agtgcttaaa cgcgataaag cgccggctcg tgaaaaagca ttgaaattgc
    12661 tggagcgcgt cgggctttcc gcacatgcca ataagtttcc ggcgcagctt tccggcggtc
    12721 agcagcagcg tgtggcgatc gctcgcgcat tgtgtatgga tcctattgcc atgctgttcg
    12781 acgaaccgac atcggcgctg gatccggaga tgatcaacga agtgctggac gtgatggtgg
    12841 aactggcgaa cgaaggaatg accatgatgg tggtgaccca cgaaatgggc tttgcccgta
    12901 aagtggcgaa tcgggtgatc tttatggacg agggtaaaat tgtcgaagac tcgccgaaag
    12961 acgctttctt cgatgatccg aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc
    13021 attaatcact ctggcgcgca tacccaagtg cgcgccatct cacgctttat cgtttcgctc
    13081 ctcgtatctg ttacatccta gcggtaccct tgtcgcaaag aagcacacaa caaggagcaa
    13141 caatggcact gccaattctg ttagattgcg acccaggtca tgacgacgct atcgcaatag
    13201 ttcttgccct cgcctcacca gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc
    13261 agacaccaga aaaaacctta cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg
    13321 atattccggt agcaggcggc gcggtaaaac cgttaatgcg taatttgatt attgcggaca
    13381 atgtgcacgg cgaaagcggt ctcgacggcc cggcattacc ggaaccgacg ttcgcaccgc
    13441 aaaactgtac ggcggtggag ctaatggcga aaacgctgtg tgaaagtgag gaacctatca
    13501 ccattgtttc aaccggaccg caaactaacg ttgccttgct gctcaatagc cacccggaac
    13561 tgcatagcaa aattgcccgt atcgtgatta tgggcggcgc aatggggctt ggtaactgga
    13621 cgcctgcggc tgaatttaat atttacgttg acccggaagc ggcagaaatt gtcttccagt
    13681 caggaattcc ggtggtgatg gccggtctgg atgttactca caaagcacaa atccacgttg
    13741 aagacactga gcgtttccgc gcgattggca accctgtttc aaccattgtt gccgaactgc
    13801 tggatttctt cctcgaatat cataaagacg aaaaatgggg ctttgtcggc gcaccactgc
    13861 atgacccatg caccatcgcc tggctgttga aaccggagtt gtttaccact gttgagcgct
    13921 gggttggcgt ggaaacacaa gggaaatata cccagggtat gacggttgtt gattattatt
    13981 atctgactgg caataaaccg aatgccaccg taatggtcga tgttgatcgt cagggctttg
    14041 ttgatttact ggctgatcgt ctgaaatttt acgcttaatc actcgccgga tgacatcacg
    14101 ccatccggca ccactcattt cattacggct cgaacggacg tctctggaac tgatcatgac
    14161 cacatttcgg gcatagcgtc agcacttccg gtgtatagat cgggagatgg aagtgacatt
    14221 tctcgcagac cagatttccc agccctacca cttctccgct gtgataaacc ccgtgatgat
    14281 tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgatg tctgccagct
    14341 cctgccacaa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
    14401 tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc gtcagctcat
    14461 cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
    14521 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac
    14581 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
    14641 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat
    14701 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
    14761 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
    14821 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
    14881 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
    14941 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
    15001 taactacacc atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct
    15061 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
    15121 caccgcaccg gcaccgtgga cgtacgacaa catcgcgtac atgaaaaacc agctcaaaat
    15181 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
    15241 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc
    15301 tgcagtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
    15361 ctgctgctgg cgctgcgata ccaaagttga gcgtaaagag atcccgcaat ggtttatcaa
    15421 aatcaccgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
    15481 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg aaatcacctt
    15541 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
    15601 gggttgtacc tacctggcgg tggctgcggg tcatccgctg gcacagaaag cggcggaaaa
    15661 taatcctgaa ctggccgcct ttattgacga atgccgtaac accaaagttg ccgaagctga
    15721 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagcggttc acccattaac
    15781 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggaatacg gcacgggcgc
    15841 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
    15901 gaacatcaaa ccggttatcc tggcagctga cggctctgag ccagatcttt ctcagcaagc
    15961 cctgactgaa aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc
    16021 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
    16081 ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat
    16141 ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct
    16201 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
    16261 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
    16321 ggagtcctcc tggtactatg cgcgctacac ttgcccggag tacaaagaag gtatgctgga
    16381 ttccaaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
    16441 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
    16501 gaactctgac gaaccagcaa aacagttgct gtgtcagggt atggtgctgg cagatgcctt
    16561 ctactatgtt ggcgaaaatg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
    16621 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
    16681 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
    16741 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
    16801 gactctcgaa tggcaggaat ccggcgtgga aggggctaac cgcttcctga aacgtgtctg
    16861 gaaactggtt tacgagcaca cagcgaaagg tgatgttgcg gcactgaacg ttgatgcgct
    16921 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga
    16981 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
    17041 caaactggcg aaagcaccga ccgatggcga gcaggaccgc gccctgatgc aggaagcact
    17101 gctggccgtt gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca
    17161 ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
    17221 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgcaaaaat
    17281 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
    17341 ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac ccggtaaact
    17401 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg
    17461 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag
    17521 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
    17581 cgtgcggtgc gtaaccagtt acgtctaaat ggtgtcgagt tgcttgataa agaaaccacg
    17641 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
    17701 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtcaa tgcgaccgtg
    17761 ttgatccccg gccgtgacat ctacccgatt agcgccaaag tcttccgttc gttcttcgat
    17821 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
    17881 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
    17941 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctct
    18001 accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
    18061 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
    18121 aggacgctat tcgtcaggta gctgcggtac aaggattcga agaacaccac actttttcca
    18181 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggccatg agtctgtttg
    18241 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
    18301 aacaacttct cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggca
    18361 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
    18421 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgtgcgcg
    18481 caaaacagct caacttagag ctggatgacg cggcgaatca ggtgctctgc tactgttatg
    18541 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctactc tggccagacg
    18601 gcaaactgac attacctcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
    18661 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
    18721 aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactgt
    18781 tgttactggt taacctgaaa cgccagtctg cccatatgcc actgcgtgcg ttgtttgata
    18841 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagcc
    18901 atccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
    18961 attacggtca gtcagtgtgg gcagagcttg aagggttatc tcttctgttg tgccataaac
    19021 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
    19081 acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc gaatttgatt
    19141 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
    19201 gcaaacagca tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
    19261 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
    19321 gagtggcggc aggagcaagg accggacgtg ccgctggcgt ttattattgg tcaggattca
    19381 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
    19441 gtctgtcggc gtcctggtta cccacttgaa atggcacaac cacaatatca gcaatggctg
    19501 gaggatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
    19561 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaat
    19621 ggtgaatcgt gtgaggattt attaccggaa ccggtactga cttacattaa ccaacaaggc
    19681 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
    19741 gtgaaacgca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg
    19801 cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatgatgtt tcctttgacc
    19861 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt cttagtgacc
    19921 gccagcaccc cgttcatatc atccctgaac tcaatgaaat gttttttggc gactgggaga
    19981 tgcgacatca tcgtgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
    20041 actggcagca tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
    20101 aacgatttat cgcaaggctt agtgaatatc agcactatca gaatatttta gtcgtcagcc
    20161 atcagggcgt actgagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt
    20221 ggcatttccg cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga
    20281 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt
    20341 ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca
    20401 gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
    20461 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
    20521 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
    20581 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
    20641 ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca gcatcaccga ctgcatgatc
    20701 atctgtacgg gtacgtccag ccgccatgtt atgtccattg ctgatcacgt tgtgcaggag
    20761 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
    20821 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
    20881 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
    20941 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
    21001 cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc
    21061 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
    21121 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
    21181 aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc
    21241 tgtaaagcgg cggcggagca gagctggtcg ctgtcggcac ttacccttcc ccatccgctg
    21301 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
    21361 tatcaccgtg agtgataagt gagctttgag tagaaaacgc agcggatgaa actacagaac
    21421 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
    21481 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
    21541 cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgca
    21601 cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggctctcaa ccgcactatc
    21661 taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg
    21721 cgcagcgtgg ttgatctgac cgatgatgat attgcggcat tccgaaaaga gcgcgcacgt
    21781 tcacaccgtt tcacctctat tccggtgaaa actaacctga ccgaagtaca agtagctcgc
    21841 tttgccgtca accagtaccg ttttccgggt gttgaagtta aaggctataa acgtcgttac
    21901 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
    21961 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc
    22021 ggtaagctgg gcattgaacg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
    22081 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
    22141 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
    22201 ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgttggcg
    22261 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
    22321 tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
    22381 tatcctcccg cgtctacagt taaaccctat gttgcggttt cggcattgag tgccggggtg
    22441 atcacgcgca atacgacgct gtttgacccc ggctggtggc aactgccagg gtcggaaaaa
    22501 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
    22561 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
    22621 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
    22681 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
    22741 ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg
    22801 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg
    22861 agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc
    22921 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
    22981 cctaacggta cggcgcataa atattttgcc agcgcaccgt acaaaattgc ggcgaaatcc
    23041 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    23101 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    23161 gctgtcgcca tgattctgga gaacggtggc gcgggtccgg cggttggtac gctgatgcgc
    23221 cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca
    23281 gcggttgccg cagcggagga ccattaatca tgacagataa tccgaataaa aaaacattct
    23341 gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca
    23401 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg
    23461 gccagattgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    23521 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    23581 ctttcggtgc catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc
    23641 aaccgtcgga aattgccaag atagccgtac cgttgatggt tgcacgcttt atcaaccgcg
    23701 acgtttgccc accttcattg aagaatacag ctatcgcgct ggtgctgata tttatgccca
    23761 cgctgctggt ggctgcacag cctgacttgg gaacatcaat cctcgttgcg ctttccggcc
    23821 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    23881 tagcggcgtt cattccgatt ctgtggttct ttctgatgca tgattaccag cgccagcgcg
    23941 taatgatgct cctggacccg gaatcagacc cactcggcgc aggctatcac attattcagt
    24001 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt
    24061 cacagcttga atttctcccc gaacgccata ccgactttat cttcgcggta ctggcggaag
    24121 agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg atcatgcgcg
    24181 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa
    24241 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    24301 ttgtaggggt tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg
    24361 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    24421 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    24481 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    24541 aacggcccca tagttgaaat tagcggggca gacccgcgtt tcgaaccact gaacgcgacg
    24601 gcaaatcagg attaccagcg cgacgggaaa agctacaaaa tcgtgcagga tccgtctcga
    24661 tttagccagg cgggactggc ggcaatctat gatgccgaac ccggcagtaa cctgacggcc
    24721 tctggcgaag ctttcgatcc gacacagttg acggcggccc atccaacgct tccaatcccc
    24781 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    24841 cgcggccttt atggcaacga ccgcgtgatt tcgctttctc gcgcggcagc tgaccgtctt
    24901 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    24961 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgccagca
    25021 cctcccgatt taagcggtgg cgcgggaaca agttcagtgc ctgtcccgca gggtgacatt
    25081 cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagc
    25141 agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg cagcgaaccg
    25201 acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcacctgca
    25261 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    25321 gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    25381 gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc
    25441 agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    25501 tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    25561 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    25621 cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    25681 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    25741 acatgccgat gacctgaata tcaaaaccat gatcccgggt gtaccgcaga tcgacgcgga
    25801 gtcctacatc ctgattgact ataactccgg caaagtactc gccgaacaga acgctgatgt
    25861 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    25921 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    25981 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    26041 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat
    26101 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    26161 cgccctgggc ctgaaaaata cccacttcca gacggtacac ggtctggatg ctgatggtca
    26221 gtacagctcc gcgcgagata tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa
    26281 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    26341 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    26401 caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatctctgc
    26461 ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaat tgctaacctg
    26521 gggcttccgt ttcttcgaaa ccgttaatcc tctgaaagta ggtaaagagt tcgcctctga
    26581 accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    26641 gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    26701 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    26761 caaaacgatc gaacaacgcc cgctggttgt gctgcaagaa atcccggaag gtaacttctt
    26821 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    26881 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaac tcccgccttc
    26941 tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg
    27001 aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc
    27061 tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg
    27121 taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata
    27181 tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc
    27241 tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga
    27301 tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa
    27361 attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa
    27421 ttcaccgata cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg
    27481 gtattcaccc aagggcaggc aggaaaggcg gagcacattt taatgccggg tgatattccg
    27541 gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg
    27601 tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt
    27661 gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac
    27721 gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc
    27781 ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt
    27841 attaatcctt gtgggtatgc aggaatggaa atggctaaaa tatcacaatg gaaacccgaa
    27901 gcgacgacta ataatattgc accacgttta ctggaaaata ttttagcgct actaaacaat
    27961 cctgacttcg aatatattac cgcttaattc catacatcaa tggcccaatt tacactgggt
    28021 cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata ttttctttat
    28081 cacttgcaga aatgcgatgc tacgccttgc tccgtagccc acctaattga ttaacccatt
    28141 gaaataattg accatggtga gcaaaagcgt tccgtctcaa ttagacataa gattattgta
    28201 atttttttaa atattatttt ttcatgaata aaattggagt aagtgcgtgg atagtaataa
    28261 tcaaattgaa ccctgtttaa gcagaaaatc gtcagaaggt aaaccacaaa tatttacaac
    28321 cttacgaaat attgatctta cccttctgac tattttgaag ttgcatatgt ccataaagga
    28381 atcgttaatg cagcgaaagt gcttaatctg cccccctcgg caatctatca gcctattcag
    28441 aaactgcgcg ttatattccc tgacctattg tttattcgca aaggccaggg tgtcactcct
    28501 accgcatttg cgatgcatct atatgagtct atcactcagg gacttgagtc cattcttggc
    28561 gcgctggata tcgaaggaag ctatgataag caacggacga taactattgc gccctccccc
    28621 tcggtcgggg cccaggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccacag
    28681 cttttactgc gcaacccgcc catcggcggc gcggaaaacc aactctgtca gtttcaaacc
    28741 gatctcatca ccgataacat gttttgcaca ggaccataga tgttatccgt aatgcatttt
    28801 gaaaaagtat tgttacagcc agatcacagc gcagaacgat aaaatggcac aatccaacaa
    28861 caattttaca ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc
    28921 cggtcgttaa ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc
    28981 ttcgtaattc gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcggt
    29041 gttaaatacc gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaacagag
    29101 cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca
    29161 cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa
    29221 gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc
    29281 ggcgctattt gtacccgccg ttgtccgttc tgtgacgttg cccacggtcg cccggtagct
    29341 ccggatgcca atgaaccagt gaaactggcg caaaccattg ccgatatggc gctgcgttat
    29401 gtggttatca cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg
    29461 gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg
    29521 gatttccgcg gtcgtatgga tcgtgctctg gatattctga ccgccacacc accagatgtg
    29581 ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat
    29641 tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc
    29701 aagtctggtc tgatggtggg tctgggtgaa accaatgaag aaattattga ggtaatgcgc
    29761 gacctgcgcc gtcatggtgt gacgatgcta acgctggggc aatatttgca gccaagccgc
    29821 catcacctgc cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa
    29881 gcgctggcga tgggctttac ccatgctgca tgcggtccgt ttgtccgctc gtcttaccac
    29941 gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg
    30001 tctgataaaa aacccgcttt ttgaagcggg ttttttgtat cgaacaagat attgagggag
    30061 cgtcctgctc gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg
    30121 cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg
    30181 ctccaaggtc tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg
    30241 ccgcaactac cagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata
    30301 accggcaaca gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac
    30361 acaatagcaa tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg
    30421 cggcagcgca aagcgacggt tgtttaagac gggcagttgc gcgcgtacct gacgcacacg
    30481 ttcgggcgtc acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac
    30541 gccaaaggga tctataatcc ggctttgccc gatatttttg ttcccgcact cccccgccgc
    30601 caccatataa caggtggtat ccagcacacg agcggcaagc aacgttgacc agtgatgctc
    30661 tttgagcggc ccacgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc
    30721 ctgtgccagc gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat
    30781 cccttccacc tccagtaacg gagctatttc attaccagca tcaacatggc gtgattcctg
    30841 aatggcaaat gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg
    30901 tagcgccacc agcatattcc acgcgcgccc cggtgttgaa ggaacatgaa tcgtcagaat
    30961 tgtcgtcatc atgttacgtt tactttctcg ccgtaaacgg tcgaggaatt cgccttccag
    31021 cagctgtgct gatttaaccg aaagatccgc atcatgatca tcacgcgcca gtaatgcttc
    31081 cggcaggaca aacagcgaaa cctcgttttc cgccgcctgc gccatcaacg aggcacaaat
    31141 ctcagcgttc ttttcccaca cagatgtaac agcaaactgt cctgcggcaa ccagcattgc
    31201 cacctcctgt cagagtcgta aaattcgcca acggcgatta ttcggcgtta cacttatcac
    31261 tcatacaaat caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg
    31321 gcggtggtac gggaagcgtg gcgagatggc tgttaagtat gcgatttaac ccgctgcatc
    31381 aggcgattcc gttggggacg ctggctgcaa acctgattgg ggcattcatc ataggaatgg
    31441 gattcgcctg gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca
    31501 ccggattttg tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt
    31561 tacaagaggg ccgctttggc tgggcgttac tgaacgtttt cgtcaacctt ttagggtctt
    31621 ttgccatgac cgcactggct ttctggctgt tttccgcctc aaccgcacac taaagaccaa
    31681 aaaaaacccg ctgattaagc gggttttgaa ttcttgctga cgtatcttac agagcgatta
    31741 cgtttgcagc agaagggcct ttggcaccgt tagtgatttc gaactctacg cgctgacctt
    31801 cagcaagagt tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc
    31861 tgccgtcttc cggagtaatg aaaccgaatc ctttggactc attaaaccac ttaacgttac
    31921 ctttaatctt agacatcaaa attaccttta catgaaaaat agacacaaat gctgtgtcgg
    31981 ttaccagtac accaattgtg gtacgctttg tccagtgcaa ctttactaaa aagtgataaa
    32041 agtcgcgtta tttttgatgt taattatttt acccattgat tttaaagagt ttactaaaat
    32101 ttcatttgtc tcaaaactga aagcgcatcc aggcaaagta cacattgcca ttgttatagg
    32161 tacccggaat gtaggtcatc tgaaaagtca ctggatcata acccacagag gccaatggca
    32221 gtagaaccgg gagagggatg taattccagt tatcgcgtgc cgttacgcca gcggtgaatc
    32281 ccagtcctaa atgaaaattt tcatccgcca gcggtcgcca ggtactttcc catccgtatc
    32341 cggcaatcgg ttcccatttg ttccacgagt ccttaaatgc catggcatac aggccatgcc
    32401 agtttccttt ttcatcccaa cgcgactgac caaaaccgcc accccacggt cgctcgttat
    32461 agcgatcggt tttttctttg tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat
    32521 ataaatcata atgttcaggc tgttgccagg tttgtgcaat attttctcta aacgttgtca
    32581 tccactcatc tgcgttagca aaaactttac caacgctgat taactgaata aaaacaaagg
    32641 aaaagatagc gacatattta ctcacgttca tttgtgacca taaaacattt atcaaaaatc
    32701 tactactagc atagcaaagc tacaattaac ataaccttaa tagccccaac ataaagaata
    32761 atctgaatag ctgctctatt tatttccgct tgtttaagtt aaatatttcc gcgcatcgat
    32821 taaagatgat gcgtcgtttt ctgcgatggg aatagtcaaa aaagaaaaat caagtctttt
    32881 ttgatgacaa aatgcaatca aggaaaaatt attttatttt ttaacgagat acaacaatca
    32941 tcttaacgaa gtatataata tatatcgtca acctatgctc atattagatt attttttgag
    33001 cgcacagccg cagctaacac cattgcaatt aacaaatttg catcaatcca ccatcaattt
    33061 acacacatta ttatgtgata attgccaacc gctaaatatg cgttttgtta tctatgtata
    33121 aaaacagcaa cttcaatgtc ttaatggcag tttttcttga ttttaatcag cattcatcgc
    33181 caatttattg ggcatatttt ttccttaagc tttaggaatt ttttattatt tactttgggg
    33241 cctggagaca ggaaaaatta tgctgacatt cattgaactc cttattgggg ttgtggttat
    33301 tgtgggtgta gctcgctaca tcattaaagg gtattccgcc actggcgtgt tatttgtcgg
    33361 tggcctgtta ttgctgatta tcagtgccat tatggggcac aaagtgttac cgtccagcca
    33421 ggcttcaaca ggctacagcg ccacggatat cgttgaatac gttaaaatat tgctaatgag
    33481 ccgcggcggc gacctcggca tgatgattat gatgctgtgt ggctttgccg cttacatgac
    33541 ccatatcggc gcgaatgata tggtggtcaa gttggcgtca aaaccattgc agtatattaa
    33601 ctccccttac ctgctgatga ttgccgccta ttttgtcgcc tgtctgatgt ctctagccgt
    33661 ctcttccgcg accggtctgg gtgttttgct gatggcaacc ctgtttccgg tgatggtaaa
    33721 cgttggtatc agccgtggtg cagctgctgc tatttgtgcc tccccggcgg cgattattct
    33781 cgcaccgact tcaggggatg tggtgctggc ggcgcaggct tccgaaatgt cgctgattga
    33841 cttcgccttc aaaacaacgc tgcctatctc aattgctgca attatcggca tggcaatcgc
    33901 ccacttcttc tggcaacgtt atctggataa aaaagaacac atctctcatg aaatgttaga
    33961 tgtcagtgaa atcaccacca ctgcccctgc gttttatgcc attttgccgt tcacgccgat
    34021 catcggagtg ctgatttttg acggcaaatg gggtccgcaa ttacacatca tcactattct
    34081 ggtgatttgt atgctgattg cctccattct ggagttcatc cgcagcttta atacccagaa
    34141 agttttctct ggtctggaag tggcttatcg cgggatggca gatgcatttg caaacgtggt
    34201 gatgctgctg gttgccgctg gggtattcgc tcaggggctt agcaccatcg gctttattca
    34261 aagtctgatt tctatcgcta cctcgtttgg ttcggcgagc atcatcctga tgctggtatt
    34321 ggtgatcctg acaatgctgg cggcagtcac gaccggttca ggcaatgcgc cgttttatgc
    34381 gtttgttgag atgatcccga aactggcgca ctcttccggc attaacccgg cgtatttgac
    34441 tatcccaatg ctgcaggcgt caaacctcgg ccgtaccctg tcacccgttt ctggcgtagt
    34501 cgttgcggtt gccgggatgg cgaaaatctc accatttgaa gtcgtaaaac gcacctcggt
    34561 gccggtgctt gttgggctgg tgattgttat cgttgctaca gagctgatgg tgccaggaac
    34621 ggcagccgcg gtcacaggca agtaaatagt catgccggag gcaagtttcc tccggcttta
    34681 tcatgtcacc cactgtggta tatgcgttgt ggtctgccaa ctttgccgtg aacaatttca
    34741 gcaataatca gatggcggct ggcgcaatat tcaagataac gtctggcagt ggtgcggctg
    34801 atagtcagtg cctgcgccac cgtttctgcc gtatgttgca caccaggctc tttaaacagt
    34861 tttcgcaccg cgtttagcgt caaggggtca atgccggtcg gcagttcgtc tttgggttca
    34921 ccgcgagcat aggcattaaa catctcatcg atttgcttct ggctggcgct atcaatactt
    34981 tccagcatat gtttacgctg gcggaaacgg gttagcgttt gccccagccg ttcataggca
    35041 attggcttaa tgagataatc aaatacacca caacgtacgg cttcagacac cgtttccata
    35101 tcactggctg cagtggtaaa caccacgtcg ccgggataat gcgcctgcac cagttcatgc
    35161 agtaaattaa tccctctacc gtcaggaaga tagttatcga gcaagattag ccccggctta
    35221 aaacgctcga tcatcattcg ggcctgcgcc aggtttcccg ccagtaatat ctgactgaat
    35281 ccgggaatgt gacgaatata ttccgcatgc atctctgcca gcggcgtttc gtcctcaacg
    35341 atcaataggg ttaatggagc tgtcattagg tttcactttc ggaatataga ttgaaaataa
    35401 ggtaccgcag ggatcattat cttcgagagt gataacacca ccgcagcgcg ttacgtagct
    35461 ggcaatcaag tacaacccaa tgccatgttc gccgggctcg tcagcacgcg tactgacacc
    35521 ctgctcaaat attttgtctc gtagagactc tggaacgccg cagccctgat cggcgacttc
    35581 aatcaccaca tcatcgcctt catcgctgag gaataattca acgatcttgt ttccttcatc
    35641 gctacgcagg ctggcttcga aggcgttatc aagtaaattg ccaacaatgg ctgcaaactc
    35701 ggtgctgtcc agtcctggcg gcagttgcga aagctgactt ccggggacga tgaccatttt
    35761 tagccccagt tcgcgggcgc gctgcacttt accaaaaagc agccccgcca cctggcgatc
    35821 ggcaaacgcc tcgcgcaggc tatcaataag ctgttgctgg gcctgagact ccccctgcac
    35881 catcgccaac acgcgatcat actctttcat ctgcaacaga ccattgagcg tcgacatcca
    35941 attgagatgc tcgtgtcgca atgtgcgcag gctctcgacg tattgtttaa tttgcgtcag
    36001 ttgtgcattg agggtggata tttcgtcttt actacgaaag ctgataatgg cccccagcaa
    36061 atcatcacca gaatgaatag cttcccggtt ggcaataacg ctcagaccgt taaagttcgc
    36121 caccacatcc tgacgttttt catcaatctg ttcggtaaag aaatcggcgg gcctgaccac
    36181 ttcagcaatg ggtttaccca accattgccg tccgggggag ctcagcccca gcatctttct
    36241 tgcgttacga ttgatggcgg taatgtaacc gtgcggatcc accgcaatga gcccttcata
    36301 aaccgaacta aacagcgcct cttgctgacg gaccacgcgt gcgatttgct ttggctccat
    36361 gcccatcatc tgccgacgga tatgcgcggc caggaaccac gacagcaaca tcagaatccc
    36421 taacagcacg acaaacacgc cagccatcgg taataaaaac tcagcccgcc agctatcgat
    36481 tttactcacc aggtagccaa tcgacaccac gccgatgact tttccatcgt catcaaagat
    36541 tggcgttttg gcacgcatcg ccatgccaat tgaccctttc ccggtgatga agtagctctc
    36601 ccctttctcc agcgcgcccg gtttggtgaa ctgcatagga taaccaattt tctccggatt
    36661 aggatggtaa agacgaatcg agtgccgatc gccaatcacc acataatcga aatcagtatc
    36721 tctttgtaat ttatcagcga tggtcgccag cctcttgtag tcacgcgttt tcactgcgct
    36781 gatgatactg tcattagaag caataatttt tgcctgattc attgccatat cgcgcacatg
    36841 aagcgttaaa tagtcctcaa aactggccgt aaaatattgc gccagggctg caatgacaaa
    36901 tattgagaac accagaatca gcaaaaagat acgcagcgga aatgccagtc tttggaaaaa
    36961 tgcaaactgt ttattctcgt taagctgcaa cattgttttc cttgcgggtt gtcccgagcc
    37021 tctttgacga atgtaattaa aatgttgaat caaaatacat agatattaaa tataatcgtg
    37081 gcaaatttta ttcattaaat caattaaatc acttaaacct gcgggtcatt tcgtaattac
    37141 attaattaaa aaacttaaaa ccattaaata aataaaaaac cactaactct atgtgaaata
    37201 aatcaaaatt tcacgctgca atactcctta ggatgtatag cgaaaggaga aaaagatata
    37261 cctcgatcgc ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacatcgaa
    37321 caacatttgt tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg
    37381 caatgatatt ttcacccgtg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca
    37441 attcctgcat gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac
    37501 ccgcgatgaa aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc
    37561 tatcagtgaa tccgttcgcg gtgaaggact ggcgctgaca ttagccaccg agctaattaa
    37621 cctcgcctat gagcggcaca gcacgcatct atttatttat accaaaaccg aatacgaggc
    37681 gctgttccgc cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat
    37741 ggaaaacagc gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat tttgtcatcc
    37801 agggaacaag attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta
    37861 tctgattcaa caggctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga
    37921 ttcttcacgc ttcccctatg aagaccgact ggatctggtg ttaaaaggca ccgccgatat
    37981 tccacgcctg actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg
    38041 ctacttcatt aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat
    38101 tttccgtcag tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc
    38161 cttttgtcgc gttactgccc agtacaacca ggatatgcgc tactggctgg aaacaccgac
    38221 tatctccgca ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc
    38281 gatatccgct tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc
    38341 gctggtccct gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga
    38401 cgcggcagct cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag
    38461 cccgccgttg caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat
    38521 acgcaggata tcgacctgca aatcaacagc agcgttgaga aacagtttgg cgatgcaatt
    38581 cgcaccacca ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc
    38641 gatgacaaag gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc
    38701 gccagcggta tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac
    38761 aacgtaaaac tcgcacccgc cgcagtatgt tgtttgtgcc tggtgctaat gccgcgatgg
    38821 tcagcaactc ctttatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtag
    38881 cattgcgtga aaaagacacc gcccgccgca tggtttacca cgcgctgcaa catccgcttt
    38941 atcgcgatat tgaaaccatt gtgcgtgtaa acgcgctgga ttccgaatgg ggtgttaacg
    39001 acctggaagc tgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg
    39061 ctcaggatgt tctggatatc gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg
    39121 aacccggcag taccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcgg
    39181 tagaaatcgc tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg
    39241 tgcgcaacct gcgtacagaa cgctccccgg aaggaactga actgctgttc gctcgctgct
    39301 ccattttgca ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg
    39361 ctaacaacga agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg
    39421 gcaaatcgct gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc
    39481 agaaagaagt ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag
    39541 gcctcggcgt ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc
    39601 gtctggtgct ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg
    39661 acgcagaaaa ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct
    39721 gaatgcgatc ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg
    39781 cgcgatcgca aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac
    39841 ggcatgacgg tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg
    39901 atggacgtca tcgcgaagat gggctttaaa aacctgactc tggcctccag ctccctgagt
    39961 gattgccatg cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc
    40021 tctggcctgc gcggtccact ggcggaagag atctcccgtg gtctgctggc ggaaccggtg
    40081 caaatccact ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgat
    40141 gtggctttcc tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctactccggt
    40201 aaagcctgct gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc
    40261 gtgatgctta ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat
    40321 caggttgatc tgatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt
    40381 gctacccgta tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg
    40441 attgttaact ctggctactt caaagaaggt ttctccatgc agaccggcac cggcggcgct
    40501 tcgctggcgg taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac
    40561 ttcgccctcg gcggtattac cgcaacgatg gtttatctgc acgaaaaagg tttgatccgc
    40621 aaactgctgg atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc
    40681 aatcacatcg aaatcagcgc caaccagtac gctaactggg ggtcgaaagg cgcagcggtt
    40741 gatcgtctcg acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac
    40801 gtgctgaccg gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg
    40861 attgcttctg cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgacgctg
    40921 gtggataacg tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac
    40981 cacggcatcg ctgttaaccc ggcacgtccg gagctggcag aacgtctgca ggaagcgggc
    41041 attaaagtgg tttccattga gtggctgcgt gaacgtgcgc gtctgctgac cggtgaacca
    41101 cagccgattg aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg
    41161 atcgatgttg tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc
    41221 caccatgcgg tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa
    41281 cacgtctggc tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg
    41341 ccgattaaag acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt
    41401 gccttagccg caaaactggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc
    41461 gggccggagg gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt
    41521 gagcttgaac atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacacccgaa
    41581 ggcgaaattc tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag
    41641 caaagcgcag ctgtttgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc
    41701 atggaggcgc tgctgaacga tgtcgatgcc tgcaacgtca actaaaaaca caaagcttgc
    41761 gacgtcatta atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa
    41821 tctgtcaccg aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc
    41881 gctggaagat ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga
    41941 atttggtgcc tgtagtgcag aaatggcacc ggaagcggta ctcaacggat tacgcccaat
    42001 tggtatggct tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg
    42061 aagcattttt tctttagggc tactgtgtgc ggcaattggt cgtttgcttc agctcaacca
    42121 gccggtaacg ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga
    42181 tcgcgaactg cgtaccaata attcacgact gacggcaggt caacggttgt accagcagct
    42241 tggccttacc ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc
    42301 cttgccgcat tacctcactc tgctggatca ggggttagat cctgagctgg cattgctcga
    42361 taccttgctc ctactgatgg ctaccaacgg cgataccaac gttgcatcgc gcggtggcga
    42421 ggggggcctg cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg
    42481 aacccccgcc gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct
    42541 cagtccaggc ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt
    42601 atttaagcac ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca
    42661 aaagataata tatggaaatt attggcgccc ctggtggtga tgggtgtcat gtttcttatc
    42721 cctgtacctg acggtatgcc gccgcaggcg tggcattact ttgcagtgtt tgtggcaatg
    42781 attgtcggca tgatcctcga gccaattccg gcaacggcga tcagttttat tgcggttact
    42841 atttgcgtta ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg
    42901 tttaatgcgc aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta
    42961 tggctggtat ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggccgt
    43021 cgtattgccc ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg
    43081 attgtcatta tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg
    43141 ggtacggttt ttccggtcat taaaaacctg ccgccgttgt ttaaatcatt cccgaacgat
    43201 ccgtccgcgc gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg
    43261 agttcgtcca tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc
    43321 aaaattgccg gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg
    43381 gttatcttgc ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca
    43441 cacagtgaag aagtggcaac ctgggcgggt gatgaactaa aaaccatggg tgcgctgaca
    43501 cgcagagagt ggacgctgat tggccttgta ttgctcagct taggtttgtg ggtatttggc
    43561 agtgaagtca ttaatgctac tgcggttggt ctgctggcag tttcgctaat gctggccctg
    43621 cacgttgtgc cgtggaaaga cattacccgc tataacagcg catggaacac gctggtcaac
    43681 ctggcaactc tggttgtgat ggctaacggc ctgactcgtt ctggttttat tgactggttc
    43741 gccaatacca tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg
    43801 gttctggtgt tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc
    43861 atgctgccgg ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg
    43921 tgtatcctgc tggtgctatc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt
    43981 cctggggtga ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc
    44041 gcaatcttcg gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc
    44101 atgtggaact aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaaaccc
    44161 acctggcggg cttttttatg attaaataga taatattcag tgtatgtatg agttccacac
    44221 ccattatgaa agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct
    44281 cttctgccaa cgccgtggcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc
    44341 tggccctctc ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac
    44401 gagatgaatg tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg
    44461 gtctgtggcc aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga
    44521 tgcgcttcgg ttgcgctact cgcccaatcc cgaatctacc agaagcgcgc gccagccgaa
    44581 tgtgttcatc gccggaaacc gggttatcac tggaaacggc cactaaacta agtgaagtca
    44641 tgccaggagc tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct
    44701 gctttggttt tgatccagac gcatacttcg gcacgatggt acgcctgaat caagaaatta
    44761 aagaaagcga agccggaaaa ttccttgcgg ataactacgg taaaacagtg agccgccgtg
    44821 actttgatgc cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa
    44881 cgtgccaggg taaccctgcg tatttgactg aaattcagat ctcggtcaaa gctgacgcca
    44941 tcaacgctcc gctttctgcc aactcattct tgccacaacc acacccaggc aactgtggca
    45001 aaacctttgt gattgataaa gcaggttatt aatcctctga tttacaaaac acgctaattc
    45061 gtcttccata aaccatgacg attaagattt atacataatg cgctacattg aggaattccc
    45121 tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa
    45181 accctcgccg cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag
    45241 taccgacgac atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc
    45301 gaacgcatcg atattctgct ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg
    45361 ttaaacgcag agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg
    45421 gtgacaatga acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc
    45481 acgctggtgt atccggcaaa aatgaccgac agcaatactc agctttccgt tatggctccg
    45541 gtaggtgccg cactgctggg gctgcgcgtt ggcgattcca ttcactggga acttccgggc
    45601 ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgac
    45661 tacctgcttt aatcccatct gcacaagcga taactcgctt tgcccagagg atgcactcgc
    45721 atcctctttc cgctcaaata tcaaccagat ttatcatttt gctcttaagc gcagcattct
    45781 aatctgaact ggctattcat ctgcggaacc tcatatgagc gaagctctcg gtatcactgc
    45841 attttatata ttcatagcag caattattgt tgctgcagtg ctataccttg aacaacgctg
    45901 gtagcgcctg gtttttattt attttccagc tcaatagcaa attcagatca aatcagcctc
    45961 gcaaattata gattcgtaac caataatgtg ctgttatgta tacatgcaaa aacaaggaga
    46021 gaatgcttat gtacaaaaca atcatcatgc cagtggatgt tttcgaaatg gaattgagcg
    46081 acaaagcgat tcgccacgct gaattcctcg cccaggatga cggagttatt catctacttc
    46141 acgtactacc ggggtcagcc agcctgagcc tgcaccgttt tgccgctgat gtacgtcgtt
    46201 ttgaagagca tctgcaacat gaagcagaag aacgtctgca aacgatggtc agccacttca
    46261 ccatcgatcc ttcccgcatt aagcaacatg tccgttttgg tagtgtgcgg gatgaagtta
    46321 acgagttagc gaaagaactt gatgcagatg tagtagtgat tggttcgcgt aacccatcaa
    46381 tttcaactca tctgttgggt tctaacgcct cgagcgtaat ccgccacgcc aatttgccgg
    46441 tgctggttgt gcgttaattg ccagaataag tctcccgccc tgcccggagc catccctgca
    46501 gggcttttta atgcaataaa aaagccgcca ggtttgaccc cggcggctaa gcaattacag
    46561 gtgaatctta cttcttctta tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga
    46621 gaggctttgg caccttcgcc agtggcgatg atgatctgct tgtacggaac cgtcgtacag
    46681 tcacccgctg cgaacacgcc tttgacgttg gtttcgcatt tcgcatcaat gataatctcg
    46741 cccatgcgat tacgttcgac tgcgccttcc agccagttgg tgttcggcag cagaccaatc
    46801 tgcacgaaaa taccggccag ttcgatgttg tgaatatcgc cgctgacacg atctcgatat
    46861 tccagaccta cgactttgct accgtcgcct ttcacttccg tggtttgcgc attcagaata
    46921 atgtcgacgt ttttcaggct gcgcagtttg tcctgcagaa cctggtcggc tttcatttct
    46981 ggcgcaaatt ccagcagcgt tacgtgctca acgatacccg ccaggtcaat tgccgcttcc
    47041 acgccggagt taccgccgcc gataaccgct acgcgtttac ctttaaacag cgggccgtcg
    47101 cagtgcgggc agtaggtcac gcctttggtg cgatactgat cttcgcccgg aacgttcatg
    47161 ttgcgccatt ttgcaccggt cgccacgata atgctgcgtg ctttcagtac cgcgccagaa
    47221 gctgtttcaa tctgatgcag gccaccttca accgctgccg ggatcagttt gctggcgctc
    47281 tggctgtcga tcacatcaac gtcgtattca tcaacgtgaa ctttcagcgc tcctgccagt
    47341 ttctggcctt cggtcttcgg tacagaaatg tagttttcga tatcaacggt atcgaggatc
    47401 tgaccaccaa aacgttcgcc catcagaccg gtacggatgc ctttacgtgc ggagtaaatt
    47461 gctgccgctg cacccgccgg gccggaaccg acgattaata cgtcataagc atcacgcttg
    47521 ttcagctctt ctgccgcacg tttttccgca ccagtatcaa ttttggcaac gatttcagtc
    47581 aacgtcatgc ggccctgacc aaactctttc ccgtttacga acactgccgg aacgcccatc
    47641 acgttgcgat cggtgatttc gttctggaag gtgccgccgt caattgcagt gtgcttgatg
    47701 cgcgggttca gtacgctcat caggttcagc gcctgcacca cgtccgggca gttgtggcaa
    47761 gagagcgagt aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc
    47821 agagactgcg cttctttcga cggatgacca ccggtccaca gcaacgccag taccagcgag
    47881 gtgaactcgt ggcccagcgg ggagcctgca aaacgtggcc cctggttaga acctgggttg
    47941 gtgatcagga aagacggctt acgcaccggc aagctgttat cttctttaaa ggtgactttg
    48001 tctgacagtt ctgcgatttc agccaacagt tccttgattt ctgccgattt agcgctgtca
    48061 tccagcgtgg caattaactc aacaggcttg gtcaatttct caaggtaagc cttgagttga
    48121 gttttcatat ttgtgtcgag catgtttatc tcctgggctt taaacatcat catgcaagcg
    48181 gcctctgcgg ctacctggat gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg
    48241 cagcacccga agaattaggt gaatttccga agtatttcgc gccggagtta gtagcgcgaa
    48301 agacaaagga aatttagatt ttaccaacca ggtccagaga cggagccaga gttgcttcac
    48361 cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac tgtgctgctt
    48421 tgattttacg cagcaggtca gacgcgtcac ggccaatgcc ttcagcggta acttcgattg
    48481 cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga ccttcatctt
    48541 cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt
    48601 tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg
    48661 tagatactgc gtatacgtct acgcccagtt tctgcaattc ttcgtaatgg tcagcaacgt
    48721 cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag aagacgctcc
    48781 agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg aatgcctggt
    48841 ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc gtgttttcga
    48901 tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt atcaaatcga
    48961 taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga
    49021 taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc ctttggtatc
    49081 ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg acatggatta
    49141 caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat gacagtgata
    49201 ggttatgcct tttactcgac ttttgcactg actgaaaagg acaaattaat gttaaaaaag
    49261 atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc tgccccagta
    49321 aaagcgattg aaaaacaggg tattacaatc atcaaaacgt tcgatgcccc tggaggaatg
    49381 aaaggttatc tcggtaagta tcaggatatg ggcgtcacca tctacctgac tccagatggt
    49441 aagcacgcta tctctggtta catgtacaac gaaaaaggtg aaaacctgag taacacgctt
    49501 atcgaaaaag aaatttacgc acccgccgga cgtgaaatgt ggcaacggat ggaacaatcc
    49561 cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtctt cgccgatccg
    49621 ttctgcccat attgtaaaca attctggcag caggctcgtc cgtgggtaga ttctggcaaa
    49681 gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc gacagcagcg
    49741 gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatatgaagc ctctggtggc
    49801 aagcttaagc taagcgtgcc tgcaaacgta agcacagagc aaatgaaagt gttaagcgac
    49861 aacgagaaac tcatggacga tctgggggca aatgtcacac cagctatcta ttacatgagt
    49921 aaggaaaata ccctgcaaca agccgtcgga ttacccga
//
LOCUS       DAZGTJ010000028        48962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000028 DAZGTJ010000000
VERSION     DAZGTJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.028"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..380
                     /gene="yjfI"
                     /locus_tag="ACHK8E_002015"
     CDS             <1..380
                     /gene="yjfI"
                     /locus_tag="ACHK8E_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFP0344287.1"
                     /translation="ATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQMII
                     ETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQHEEYYIVFGALSLKSSLEDI
                     LLEITSLVDNALDLAEITEEYSH"
     gene            399..1097
                     /gene="yjfJ"
                     /locus_tag="ACHK8E_002016"
     CDS             399..1097
                     /gene="yjfJ"
                     /locus_tag="ACHK8E_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP0344288.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1147..1806
                     /gene="yjfK"
                     /locus_tag="ACHK8E_002017"
     CDS             1147..1806
                     /gene="yjfK"
                     /locus_tag="ACHK8E_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP0344289.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            1824..2222
                     /gene="yjfL"
                     /locus_tag="ACHK8E_002018"
     CDS             1824..2222
                     /gene="yjfL"
                     /locus_tag="ACHK8E_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP0344290.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2232..2870
                     /gene="yjfM"
                     /locus_tag="ACHK8E_002019"
     CDS             2232..2870
                     /gene="yjfM"
                     /locus_tag="ACHK8E_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP0344291.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2873..4036
                     /gene="yjfC"
                     /locus_tag="ACHK8E_002020"
     CDS             2873..4036
                     /gene="yjfC"
                     /locus_tag="ACHK8E_002020"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0344292.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4120..5745
                     /gene="aidB"
                     /locus_tag="ACHK8E_002021"
     CDS             4120..5745
                     /gene="aidB"
                     /locus_tag="ACHK8E_002021"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP0344293.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5853..6128)
                     /gene="yjfN"
                     /locus_tag="ACHK8E_002022"
     CDS             complement(5853..6128)
                     /gene="yjfN"
                     /locus_tag="ACHK8E_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP0344294.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6277..6606)
                     /gene="bsmA"
                     /locus_tag="ACHK8E_002023"
     CDS             complement(6277..6606)
                     /gene="bsmA"
                     /locus_tag="ACHK8E_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP0344295.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6788..7537
                     /gene="yjfP"
                     /locus_tag="ACHK8E_002024"
     CDS             6788..7537
                     /gene="yjfP"
                     /locus_tag="ACHK8E_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0344296.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7534..8289)
                     /gene="ulaR"
                     /locus_tag="ACHK8E_002025"
     CDS             complement(7534..8289)
                     /gene="ulaR"
                     /locus_tag="ACHK8E_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP0344297.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8397..9461)
                     /gene="ulaG"
                     /locus_tag="ACHK8E_002026"
     CDS             complement(8397..9461)
                     /gene="ulaG"
                     /locus_tag="ACHK8E_002026"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP0344298.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9816..11213
                     /gene="ulaA"
                     /locus_tag="ACHK8E_002027"
     CDS             9816..11213
                     /gene="ulaA"
                     /locus_tag="ACHK8E_002027"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0344299.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11229..11534
                     /gene="ulaB"
                     /locus_tag="ACHK8E_002028"
     CDS             11229..11534
                     /gene="ulaB"
                     /locus_tag="ACHK8E_002028"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP0344300.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11544..12008
                     /gene="ulaC"
                     /locus_tag="ACHK8E_002029"
     CDS             11544..12008
                     /gene="ulaC"
                     /locus_tag="ACHK8E_002029"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP0344301.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12022..12672
                     /gene="ulaD"
                     /locus_tag="ACHK8E_002030"
     CDS             12022..12672
                     /gene="ulaD"
                     /locus_tag="ACHK8E_002030"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0344302.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12682..13536
                     /gene="ulaE"
                     /locus_tag="ACHK8E_002031"
     CDS             12682..13536
                     /gene="ulaE"
                     /locus_tag="ACHK8E_002031"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP0344303.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13536..14222
                     /gene="ulaF"
                     /locus_tag="ACHK8E_002032"
     CDS             13536..14222
                     /gene="ulaF"
                     /locus_tag="ACHK8E_002032"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP0344304.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14351..14626)
                     /gene="yjfY"
                     /locus_tag="ACHK8E_002033"
     CDS             complement(14351..14626)
                     /gene="yjfY"
                     /locus_tag="ACHK8E_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP0344305.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14954..15349
                     /gene="rpsF"
                     /locus_tag="ACHK8E_002034"
     CDS             14954..15349
                     /gene="rpsF"
                     /locus_tag="ACHK8E_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP0344306.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15356..15670
                     /gene="priB"
                     /locus_tag="ACHK8E_002035"
     CDS             15356..15670
                     /gene="priB"
                     /locus_tag="ACHK8E_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP0344307.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15675..15902
                     /gene="rpsR"
                     /locus_tag="ACHK8E_002036"
     CDS             15675..15902
                     /gene="rpsR"
                     /locus_tag="ACHK8E_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP0344308.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15944..16393
                     /gene="rplI"
                     /locus_tag="ACHK8E_002037"
     CDS             15944..16393
                     /gene="rplI"
                     /locus_tag="ACHK8E_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP0344309.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            16795..17286
                     /locus_tag="ACHK8E_002038"
     CDS             16795..17286
                     /locus_tag="ACHK8E_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0344310.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            17289..18557
                     /locus_tag="ACHK8E_002039"
     CDS             17289..18557
                     /locus_tag="ACHK8E_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFP0344311.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
     gene            complement(18700..19977)
                     /locus_tag="ACHK8E_002040"
     CDS             complement(18700..19977)
                     /locus_tag="ACHK8E_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0344312.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWMLIKVKDEEPQVQLATS"
     gene            complement(20070..22142)
                     /locus_tag="ACHK8E_002041"
     CDS             complement(20070..22142)
                     /locus_tag="ACHK8E_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP0344313.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(22139..23680)
                     /locus_tag="ACHK8E_002042"
     CDS             complement(22139..23680)
                     /locus_tag="ACHK8E_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFP0344314.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(23690..24466)
                     /locus_tag="ACHK8E_002043"
     CDS             complement(23690..24466)
                     /locus_tag="ACHK8E_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFP0344315.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(24476..25318)
                     /locus_tag="ACHK8E_002044"
     CDS             complement(24476..25318)
                     /locus_tag="ACHK8E_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0344316.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(25328..26119)
                     /locus_tag="ACHK8E_002045"
     CDS             complement(25328..26119)
                     /locus_tag="ACHK8E_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFP0344317.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26332..26979
                     /locus_tag="ACHK8E_002046"
     CDS             26332..26979
                     /locus_tag="ACHK8E_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0344318.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(26963..27601)
                     /gene="ytfB"
                     /locus_tag="ACHK8E_002047"
     CDS             complement(26963..27601)
                     /gene="ytfB"
                     /locus_tag="ACHK8E_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP0344319.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            27819..28439
                     /gene="fklB"
                     /locus_tag="ACHK8E_002048"
     CDS             27819..28439
                     /gene="fklB"
                     /locus_tag="ACHK8E_002048"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0344320.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            28748..30160
                     /gene="cycA"
                     /locus_tag="ACHK8E_002049"
                     /gene_synonym="dagA"
     CDS             28748..30160
                     /gene="cycA"
                     /locus_tag="ACHK8E_002049"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP0344321.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(30205..30867)
                     /gene="ytfE"
                     /locus_tag="ACHK8E_002050"
     CDS             complement(30205..30867)
                     /gene="ytfE"
                     /locus_tag="ACHK8E_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP0344322.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(30975..31940)
                     /gene="ytfF"
                     /locus_tag="ACHK8E_002051"
     CDS             complement(30975..31940)
                     /gene="ytfF"
                     /locus_tag="ACHK8E_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0344323.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(32048..32908)
                     /gene="qorB"
                     /locus_tag="ACHK8E_002052"
     CDS             complement(32048..32908)
                     /gene="qorB"
                     /locus_tag="ACHK8E_002052"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0344324.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            32997..33377
                     /gene="ytfH"
                     /locus_tag="ACHK8E_002053"
     CDS             32997..33377
                     /gene="ytfH"
                     /locus_tag="ACHK8E_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP0344325.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(33506..35449)
                     /gene="cpdB"
                     /locus_tag="ACHK8E_002054"
     CDS             complement(33506..35449)
                     /gene="cpdB"
                     /locus_tag="ACHK8E_002054"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP0344326.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            35639..36379
                     /gene="cysQ"
                     /locus_tag="ACHK8E_002055"
     CDS             35639..36379
                     /gene="cysQ"
                     /locus_tag="ACHK8E_002055"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP0344327.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(36369..36926)
                     /gene="ytfJ"
                     /locus_tag="ACHK8E_002056"
     CDS             complement(36369..36926)
                     /gene="ytfJ"
                     /locus_tag="ACHK8E_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0344328.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            37251..37457
                     /gene="ytfK"
                     /locus_tag="ACHK8E_002057"
     CDS             37251..37457
                     /gene="ytfK"
                     /locus_tag="ACHK8E_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP0344329.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(37519..38862)
                     /gene="paeA"
                     /locus_tag="ACHK8E_002058"
     CDS             complement(37519..38862)
                     /gene="paeA"
                     /locus_tag="ACHK8E_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP0344330.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(39185..39823)
                     /gene="msrA"
                     /locus_tag="ACHK8E_002059"
     CDS             complement(39185..39823)
                     /gene="msrA"
                     /locus_tag="ACHK8E_002059"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP0344331.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            40029..41762
                     /gene="tamA"
                     /locus_tag="ACHK8E_002060"
     CDS             40029..41762
                     /gene="tamA"
                     /locus_tag="ACHK8E_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP0344332.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            41759..45538
                     /gene="tamB"
                     /locus_tag="ACHK8E_002061"
     CDS             41759..45538
                     /gene="tamB"
                     /locus_tag="ACHK8E_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP0344333.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            45541..45882
                     /gene="ytfP"
                     /locus_tag="ACHK8E_002062"
     CDS             45541..45882
                     /gene="ytfP"
                     /locus_tag="ACHK8E_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP0344334.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(46001..46996)
                     /locus_tag="ACHK8E_002063"
     CDS             complement(46001..46996)
                     /locus_tag="ACHK8E_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0344335.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKALESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(47063..47362)
                     /locus_tag="ACHK8E_002064"
     CDS             complement(47063..47362)
                     /locus_tag="ACHK8E_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0344336.1"
                     /translation="MSIPQNEKLKLIRESERMKMKELSDLIGINYYTYHGYESGKSKM
                     PLDFGILLLRHARFRKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            47470..47895
                     /locus_tag="ACHK8E_002065"
     CDS             47470..47895
                     /locus_tag="ACHK8E_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP0344337.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            47926..48417
                     /locus_tag="ACHK8E_002066"
     CDS             47926..48417
                     /locus_tag="ACHK8E_002066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344338.1"
                     /translation="MIKKYSEHGSKAGEAIAIDEDCLITGDPWLATERDLEGKTFFVR
                     AIKHKDRVVGGIIYSTSTIIPDNYLDISFRPVSIQGCEAYLNYVAELKKINLDYMWPI
                     VQREIMRRKSEGFDVKIEFLSTLMGFLQKPLILFPSLDRNLPVKEGVEKYIVEQVRDL
                     VCL"
     gene            complement(48395..48718)
                     /locus_tag="ACHK8E_002067"
     CDS             complement(48395..48718)
                     /locus_tag="ACHK8E_002067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344339.1"
                     /translation="MIDKSVKDAITEYLVSIYTTNLAVAPVDKLTDPRDSVGVVSDLL
                     ISLLISELPDDKRDALMKTASEILRQIEGQQAFAPYAVNIRTRFHSINHLACFIRASK
                     DKPDL"
     gene            48826..>48962
                     /locus_tag="ACHK8E_002068"
     CDS             48826..>48962
                     /locus_tag="ACHK8E_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0344340.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNSHYSLQETHATPDGYLVK
                     QT"
BASE COUNT        12051 a        12005 c        12758 g        12148 t
ORIGIN      
        1 tggcgacggc gctgcaaact gtacctgaac aaaatattga tgtaacaaat agcgagaacg
       61 cattaattat taaaatgaat gattatggcg atttgcaaat caatattcta tttacttccc
      121 gccaaatgat tatcgaaacc tttatttgtc cggtaagtag tatcagcaat ccagatgaat
      181 ttaatacctt cttattaaga aatcagaaga tgatgccgtt gtcatcggta gggatctcca
      241 gcgtgcaaca tgaagagtat tacattgttt tcggtgcgtt atcgcttaaa tcttctcttg
      301 aagatatcct gcttgagata acctcgctgg tagataacgc attggatctg gctgaaatta
      361 cagaagaata ttcacactaa ttacaaggac gggtatttat gggaatttta aaaagtttat
      421 ttacgctggg gaaatcgttt atctcccagg cggaagaatc cattgaagaa acccagggcg
      481 tgcgcatgct ggaacagcat attcgtgacg ctaaagctga actcgataaa gccggaaaat
      541 ctcgcgttga tctgctggcg cgggtgaaat taagtcacga taagctgaaa gatttacgtg
      601 agcgcaaagc cagtctggaa gctcgtgcgc tggaagcgtt aagcaagaac gttaatccgt
      661 cgttgattaa cgaagttgct gaagaaatcg cgcgccttga gaatctcatt accgctgaag
      721 agcaagtgtt gtcgaatctg gaagtctctc gtgatggcgt ggaaaaagca gttacagcga
      781 cagcgcagcg tatcgctcag tttgagcagc aaatggaagt cgttaaagct actgaagcca
      841 tgcagcgtgc acaacaggct gtaacgacct ctactgttgg cgcatcttcc agcgtttcga
      901 cagcggcaga atccttaaaa cgcctgcaaa cgcgtcaggc cgaacgtcag gctcgcctgg
      961 atgctgccgc acagttggaa aaagtcgcag acggtcgcga tcttgatgaa aagctggcgg
     1021 aagccggaat tggcggtagc aataaaagta gcgcccagga tgtattagca agactgcaac
     1081 gccaacaggg cgagtaattt tttgccagcc tcactgcaag gctggcaact attttaagga
     1141 taaaatatgt ctggtttttt ccagcgtctg tttggcaagg ataataagcc tgctatcgct
     1201 cgcggtcctc tgggacttca tctcaatagt ggctttacgc tcgacacgtt agcgtttcgt
     1261 ttgctggaag atgaattgct gattgcgctg ccgggtgagg aatttacggt agccgctgtc
     1321 agccgcatcg atctgggcgg cggtagccag attttccgct actacacttc aggcgatgag
     1381 tttctgcaaa tcaataccac tggcggcgaa gatattgatg acattgatga tatcaagctc
     1441 tttgtctatg aagagagcta cggtatcagt aaagagagtc actggcgcga ggcgatcaat
     1501 gccaaagcga tgggggcaat gaccttaaac tggcaggaaa aacgctggca gcgatttttt
     1561 aatagcgaag aaccgggaaa tatcgaaccc gtctacatgc tggaaaaagt agaaaatcaa
     1621 aaccatgcta aatgggaggt ccataatttt accatgggct atcaacggca agtgaccgaa
     1681 gatacttacg aatatctgct gttaaatggt gaggaatctt ttaatgatct cggcgagccg
     1741 gagtggttat ttttgcgcgc gctgggggtc gatatcccgc tgacatcact tcatattatt
     1801 ggttaattac aaggacgttt acgatgcata tactggattc acttcttgcc tttagcgctt
     1861 atttttttat tggtgtggct atggtgatta tttttctgtt tatctactcc aaaattacac
     1921 cgcacaacga atggcagtta atcaaaaata ataacaccgc ggcggcactg gcgttcagcg
     1981 gaacattgtt ggggtacgtg atccccttat ccagtgcggc aatcaatgcg gtgagtattc
     2041 cagactattt cgcatggggc gggattgctc tggtgattca attactcgtt tttgctggcg
     2101 tcaggcttta tatgcccgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa
     2161 tgtttatggg aaccgccgcg ctggctggcg gtatttttaa cgcagcttgt atgacatggt
     2221 aatggacgat catggccaga aaacgcaaat ccagaaataa caaaaatatc ggacatgggg
     2281 caatcagtcg cattggtaga ccgcataatc cttttgagcc gcgccgcaat cgctacgcac
     2341 aaaaatactt aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgtg
     2401 gcgatagtgg cgatgtcgat aacgacggcg acggaacgtt ttacgcgact gtgcaggatt
     2461 gtattgatga cggtaataat gcagacattt gcgcgcgtgg ctggaacaac gccaaagcgg
     2521 cgttttatgc cgatgttccg aagaatatga ctcagcagaa ttgtcagtct aagtacgaaa
     2581 attgctacta cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa
     2641 gccgggttat tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat
     2701 ttgcttcgcg ccccgtctgg cgcaacactt ctggtgatta ctcctggcgc tccggttctg
     2761 gcaaaaaaga gtcttactct tcgggcggct ttaccaccaa aaaagcgtca accgtttctc
     2821 gcggcggcta tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag
     2881 acacaacgtt cctgtgcggc gggatctgga ccagatcgcc gctgacaacg gtttcgactt
     2941 tcatatcatc gacaatgaaa tctattggga cgagagtcga gcttaccgtt ttactctgcg
     3001 ccagattgaa gagcagatcg aaaaaccgac tgcggaactg catcagatgt gccttgaggt
     3061 ggtggatcgc gcggttaaag acgaagagat cctgacgcaa ctggcgatcc cgccgttgta
     3121 ctgggatgtg atcgccgaaa gctggcgcgc acgcgatcct tcgttgtatg gccgtatgga
     3181 ttttgcctgg tgtggtaatg caccggttaa gttgctggag tacaacgccg atacgccaac
     3241 ttcattgtac gagtcggctt atttccagtg gttgtggctg gaagatgccc ggcgcagcgg
     3301 cgttattccg cgcgatgccg atcagtacaa tgccattcag gagcgcctga tttcacgctt
     3361 tagcgagctt tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgacgaaga
     3421 caggagtacc gtgctgtact tgcaggactg cgccgagcag gcagggcagg agtcgcggtt
     3481 tatctacatt gaagatctcg gtttgggcgt cggcggtgtg ctgaccgatc ttgatgataa
     3541 tgtcatccag cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg
     3601 tccgctgctg cgcaagcgtc gcgagcaatg ggtggagccg ttatggaaaa gtatcttgag
     3661 taataaaggg ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc
     3721 atcctggttc gatggtgaaa aaccgcagat tgccgctggc gaaagctatg tgcgtaaacc
     3781 gatttactcg cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgttgttga
     3841 ccacgctgat ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc
     3901 gcgatttggc gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg
     3961 aatggggatc cgtgaagata acacgttgat caccaaagac acctcacgtt tcgttccgca
     4021 ttacattgct ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga
     4081 ccatactaat ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc
     4141 gtttttaatc aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctc
     4201 tgcgaagcgg taacgcgtga aggtgctggc tgggatagcg attttctcgc cagtattggt
     4261 cagcagttag ggacggctga atcccttgaa ctggggcggc tggcgaatgt gaatccgcct
     4321 gaattattgc gctacgatgc ccaggggcgg cgtctggacg atgtgcgttt tcaccccgcc
     4381 tggcacctgc tgatgcaggc gctatgtacc aatcgggtgc acaatcttgc ctgggaagaa
     4441 gacgctcgct ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttgca cgcacaggtt
     4501 gaggcagggt cgttatgtcc gataacgatg acctttgccg ccacgccatt gctgttacag
     4561 atgttacccg cgccgtttca ggactggacc acgccgctgt tgagcgatcg ctacgattct
     4621 cacttattgc caggtgggca aaaacgcggt ttgttgattg gcatgggaat gacggaaaaa
     4681 cagggcggtt ccgatgtcat gagcaacacc acccgcgcag agcgcatgga agatggctct
     4741 tatcggctgg tggggcataa atggtttttc tcggtgccgc aaagcgatgc gcatctggtg
     4801 ctggcgcaga ctacgggcgg tttgtcctgc ttttttgtgc cgcgcttttt gcctgacggg
     4861 caacgcaacg cgattcgcct cgagcggctg aaagataagc tgggtaatcg ctctaacgcc
     4921 agctgcgaag tggagtttca ggatgccatt ggctggttgt tggggcagga aggggaagga
     4981 attcgtctga tcctgaaaat gggtgggatg acgcgttttg attgcgccct gggtagccat
     5041 gccatgatgc gccgtgcatt ttcgctagcg atttatcatg cacatcaacg ccatgttttt
     5101 ggtaatccat tgatccaaca gccccttatg cgtcatgtct taagtcgcat ggcacttcag
     5161 cttgaagggc aaacggcgtt gctgtttcgt cttgctcgag cgtgggaccg gcgtgccgat
     5221 gccaaagaag ccctgtgggc gcgtttattt acgcctgcgg cgaaatttgt gatctgcaaa
     5281 cgaggtatgc cgtttgtggc cgaagcgatg gaggtgctgg gcggcattgg ttattgcgag
     5341 gagagcgagc tgccgcggct ttaccgggag atgccggtaa acagtatttg ggaaggttcc
     5401 ggtaatatta tgtgcctgga tgtactgcgc gtgctcaata agcaagcggg tgtatacgac
     5461 ttattgtcgg aagcatttgt ggaagtgaaa gggcaggatc gctattttga tcgcgcggtt
     5521 cgtcgtttac agcagcagct gcgtaagcca gctgaagaac tggggcgaga gattactcat
     5581 cagctattcc tgctgggctg cggtgcgcaa atgttgaaat atgcctctcc gccaatggcg
     5641 caggcgtggt gtcaggtgat gttagatacg cgcggcggcg tacggttgtc agagcagatc
     5701 cagaatgatt tattgctgcg ggcgacggga ggagtgtgtt tgtaagcgta tacgacgctg
     5761 gtttcgatta actcaatgaa atatgtgaaa attgttagcc ggacaaggcg ctcgcgccgc
     5821 atccggcatt gttcatttgc tgtagatgac actcacgcat acaggatcgc ctgtacgcgc
     5881 cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga cgcaccttgt
     5941 tcatcagcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga aatattgcta
     6001 actgagatct gacctatttc attcaggcct gtcacgcagt cagcactggc gaattctgca
     6061 gactgtgccg tggcgctcgc aggataagtc actaattgca gcgaggatga ggcaaggaat
     6121 tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt ccatcgcggt
     6181 aataaacgcc caggcttcca gccggtaatt tattgtgcac aggtaaacgc atagggacgc
     6241 aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca aaatggcttg
     6301 tgaataccac tgtcctgtga cgatggtttc gtccaccatg acgacaacgt aataatcagc
     6361 ttttgcagcg acagctttcg ctctgatttc tgctaatgcg tcatccggag aaccccgaac
     6421 catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc gaatctcttg
     6481 cggatggtcg gttactggcg gtgctggctg tggcgtacct tgtagtgcgc tacaggcact
     6541 taacatcaac accaataata aactggcaaa ccggtaaata acgctattac gtttcctgct
     6601 aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa ttgttacctg
     6661 gtacgcgatt ttcgaatgaa aacgtgatga aagcgtaacc cacatctcaa cattatgaac
     6721 taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag acaaaaagga
     6781 gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc ttcatgctta
     6841 tcctgtcggg caaaaagata ccccgttacc gtgcgcgatt ttttatcacg gctttacttc
     6901 gtccagtctg gtgtatagct attttgccgt tgcgctggcg caggctggtt tgcgggtgat
     6961 catgccggat gcgcccgatc acggtagtcg ttttagtggc gacgcagcgc ggcggttaaa
     7021 tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac gtgcggcaat
     7081 agccgaagaa aactggctgc gtgatgaccg tctggcagtc ggtggcgcgt cgatgggcgc
     7141 gatgacggca ctgggaatta ccgctcgcca ctccacggtg aaatgtaccg ccagcatgat
     7201 gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga tacctgaaac
     7261 ggcagcacag cagaatgaat tcaataacat tgtcgcgccg ctggcagagt gggaagcgac
     7321 aaaccacctg gaacaacttg ctgacagacc tctactgctg tggcatggcc ttgacgatga
     7381 cgtcgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga cgggacggga
     7441 taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc ctgaggcgtt
     7501 agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc tgaccccttg
     7561 cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca gcatatcgat
     7621 ttgatcggca cggctaaaaa gcattcccgc gcgttcgccg atcttactgc tatcaaccag
     7681 taccaccagt ttccctacca cgcttagcat cttctgctct gccattgccg tcagcatatc
     7741 ggttttatac aacccttctg ctgtcagccc tttgccgctg gtaaacatcc agtgcccggc
     7801 atagagactg ttttcgctgc cttgcgggct taaagtgatg gactgacttt tgttgtactg
     7861 tccgcccata atgatcacgc tgtcatgctc ctgatcgatc aggtaatttg ccagcggtag
     7921 ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca gaaacgcggt
     7981 ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg ccgctttagc
     8041 aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg tccagcgtgg
     8101 gcgctgctgg gtaatagctt ctgcgccatt gcgcactttt ttcagtttgc cgctttcgtc
     8161 aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct caacgacttt
     8221 ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat gtctttgtgc
     8281 ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg aaatagccag
     8341 gaaatactac gccggatagc gcgaactact ccacgctatc cggtacaagc gacagattac
     8401 aggaacgact tgaacggcag atccggttca atagtgaagc aatcatcgaa accacgcgga
     8461 tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc gacctgccag
     8521 ataaacggct tgaagccata cttcaggcga tcttttttca tctcccacag cacgcggatc
     8581 tcttgcggat cggcctggaa gtttgaccag atatcgtggt ggaacgggat cactacttta
     8641 gcattcagcg cttcgcccat acgcaagata tcagcactgg tcattttgtc ggtaataccg
     8701 cgtgggtttt cgccgtatga acctaaagcc acgtcgatct gatgctcgtt gccatgtttt
     8761 gcatagtagt tagagtagtg ggaatcgccg ctgtgataca gagaaccgcc cggtgttttg
     8821 aacagatagt ttactgcgcg atcgtccatg ccgtctggca gcacgccagc cgctttttga
     8881 tccgctggta gggtgatcag tgcagtacgg tcgaacgcgt caagggcatg aatttcaatg
     8941 tctttcactt ttacgacatc gcccggtttg accacgatgc aacgctcttt cggtacgccc
     9001 cagccaatcc ataaatccac gcaggttttc ggtccgataa acggtacgtc atcagcacag
     9061 ttctgcatca cggcagcagc gacgttgacg tcgatatgat cgttgtgatc gtgagttgcc
     9121 agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt ggtgcgcagg
     9181 tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg tttcatcagc
     9241 gggttaccgt gactttgttt gccagtaccg caccagaaat caacgcaaac gttggtgcca
     9301 ccttccgatt tcagccagat cccggtgcag cctagccacc acatcgcgaa tgtgccaggg
     9361 gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg gaaagtgctc
     9421 aggatccagg attctcgggt gatacttttc actttactca tcgccattta ccttcatgat
     9481 agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat tttttcagga
     9541 aggcaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt gagtgtggaa
     9601 agatttaatg gaatgtgtaa ttcaattaac taaatgaatt taaatgaata attatttcgt
     9661 tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca tttaatcata
     9721 aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt ccgggtgtgt
     9781 ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac aacatcttta
     9841 ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt gtgacctgtc
     9901 tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg attaaaacca
     9961 taattggttt catgttgttg caggcaggat ccggcatcct caccagcacc ttcaaaccgg
    10021 tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat acttacgcgt
    10081 cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt tacgcggtgt
    10141 tgttagcgct ggcactgaac atctgttacg tgctgctgcg tcgcattacc ggtattcgta
    10201 ccatcatgct gactggtcac atcatgttcc agcaggcggg gctgattgcc gtcacgctgt
    10261 tcatctttgg ctactccatg tggacgacca ttatctgcac cgcgattctg gtttcgctct
    10321 actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg gacggctgcg
    10381 gtttctccat cggtcaccag caacagtttg catcatggat tgcctataaa tttgcgccgt
    10441 tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg ctgaatattt
    10501 tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt gccattctgc
    10561 tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgaactgg acggtgtaca
    10621 tcctgcaaac tggtttctcc ttcgcggtgg cgatcttcat catcactcag ggtgtgcgca
    10681 tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgtctg atccctggtg
    10741 cggttctggc gattgactgt gcggctattt atagcttcgc gccgaacgcc gtggtctggg
    10801 gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc gcctgcggtt
    10861 cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc actattggcg
    10921 tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg gtgatgggga
    10981 tgatcgaaat ctttggctgc gtctgggcgg tgaaacttac cggtatgagt gcctggatgg
    11041 gtatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc tccatcggta
    11101 tcgcctttat ggctgtcatc attgtaattg cactggctta tatgttcttc gctggccgcg
    11161 cactgcgcgc agaagaagat gcagaaaaac aactggcaga acagtctgct taataaggag
    11221 ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc agttccatga
    11281 tcatgaaaat gaaagtggac cagtttttaa cccagtcgaa cattgaccac acggtaaaca
    11341 gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc atcgcttcta
    11401 cccatattgc gggcgaaatc actgtgaccg gcaacaaata tgtggttggc gtgcgcaata
    11461 tgctctctcc tgccgacttt ggcccgaaat tgctggaagt gatcaaagag cattttccgc
    11521 aggacgtgaa gtaaggacgc gccatgaaat tacgtgattc gctggcggag aataaatcca
    11581 tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc gttgacctgc
    11641 tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat gcggttgaac
    11701 agcacggtcc gtactttgtg ctcgcacccg gcctggcaat gccgcacggt cgcccggaag
    11761 agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg gagttcaacc
    11821 acgaagataa cgatccggtg gatatcctca tcaccatggc ggcggtcgat gccaacactc
    11881 accaggaagt gggcatcatg cagatcgtca atctgtttga agatgaagag aatttcgacc
    11941 gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc accaacgcgg
    12001 cagcttaaga aggaattgaa aatgtcatta ccgatgttgc aggtcgcgct ggacaaccag
    12061 actatggata gcgcctatga aaccactcgc ctgattgccg aagaagtcga cattatcgaa
    12121 gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct gaaagcgctt
    12181 tacccgcaca aaatcgtact ggcagacgcc aaaattgccg atgcgggcaa aatcctttct
    12241 cgtatgtgct tcgaagctaa cgctgactgg gtgaccgtga tttgctgtgc ggatatcaac
    12301 acagctaaag gtgcgctgga cgtggcaaaa gagtttaacg gcgacgtgca gatcgaactg
    12361 accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggcat tgggcaggtg
    12421 gtttatcacc gcagccgtga cgcacaggcc gcaggtgtgg cgtggggcga agcggacatt
    12481 accgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg tggtctggcg
    12541 ctggaagatc tgccgttgtt taaaggcatt ccgattcatg tgtttatcgc tggtcgcagc
    12601 attcgtgatg ccgcttctcc ggtggaggcc gcacgtcagt tcaaacgttc catcgctgaa
    12661 ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat ctatgaaaaa
    12721 gcgctccccg ccggggagtg ctggctggaa cggctgcaac tggcaaaaac gttaggcttc
    12781 gattttgtcg agatttcggt agatgaaacg gacgatcgcc tgtcgcgcct cgactggagc
    12841 cgcgagcagc gtctggcgct ggtgaacgcg attgttgaaa ccggcgtgcg cgtgccgtcc
    12901 atgtgccttt ctgctcatcg tcgtttcccg ctgggcagcg aagatgacgc agtgcgggcg
    12961 caggggctgg agattatgcg caaagctatc cagttcgccc aggatgtcgg tattcgcgtg
    13021 atccaactgg cgggctacga cgtttactat caggaagcca ataacgaaac gcgtcgtcgt
    13081 ttccgtgacg gcctgaaaga gagcgttgag atggcaagcc gcgcgcaggt gacgctggcg
    13141 atggagatca tggattaccc gttgatgaac tccatcagca aggcgctggg ttacgcgcac
    13201 tatcttaaca acccgtggtt ccagctctac ccggatatcg gcaacttgtc ggcgtgggac
    13261 aacgacgtgc agatggagtt gcaggccggg atcgggcata tcgtcgcggt gcatgtgaaa
    13321 gacaccaaac caggcgtctt caaaaacgtc ccgtttggcg aaggtgtagt ggatttcgaa
    13381 cgttgcttcg aaacgctcaa acagagtggt tattgcgggc cgtacctgat tgagatgtgg
    13441 agcgaaacgg cggaagaccc ggcggcagaa gtggcgaaag cgcgtgattg ggtgaaagcg
    13501 cgcatggcca aagccggcat ggtggaggcg gcataatgca aaagctaaaa cagcaggtat
    13561 ttgaagccaa tatggatctg ccgcgctacg ggctggtgac ctttacctgg ggcaacgtca
    13621 gcgccattga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt gcctacgaaa
    13681 ccatgaaagc agacgatatg gtggtggttg atatgagcgg caaggtggtg gaaggggagt
    13741 atcgtccgtc ttccgacacc gcgacgcatc ttgaactcta ccgccgttat ccgtcgcttg
    13801 gcggcattgt ccatacccat tccacgcatg ccaccgcatg ggcgcaggcg gggctggcta
    13861 tcccggcatt aggcaccacg cacgctgact acttctttgg cgatatcccc tgtacgcgcg
    13921 ggttaagcga agaagaagtg cagggggaat acgagctaaa cactggcaaa gtgattatcg
    13981 aaacgctggg tgacgccgag ccgctgcata cgccgggaat tgtggtgtat cagcacgggc
    14041 cattcgcctg ggggaaagat gctcacgatg cggtgcataa cgcggtggtg atggaagaag
    14101 tggcgaaaat ggcgtggatt gcccgcggca ttaacccgca actgaatcac atcgacagct
    14161 atctcatgaa taaacacttc atgcgtaagc acggtcccaa cgcctattac gggcagaagt
    14221 aaaacacgcg ctgcggagat ttccttcttc gggagataac tggtctaagt ccgcagccgt
    14281 ttttcaaaaa aagccccctg aaaagggggc aaagcaaact atggcaatgt ttcgttggtt
    14341 atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta ctccccggtt
    14401 cccgcatcag aatcacgtgg tagtacgttg cgccctgcgc atctgtctct tcatttaatg
    14461 cctgacgcgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg cctaagcttt
    14521 gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc catgtatttg
    14581 cacttagcaa aagcacagcc agaagggcta aaacacggct gaacatagat acctcctcga
    14641 cggctgactt tgtgtgctct cctcttgtgg tgatcttctg atttaatttt aatcaatgat
    14701 aaagaagtgg atggtgacca tttctgatgc agttgttcaa aaaaacacca tgatgaagtg
    14761 tgatgaactt caaatcagcg tgttagtacg atttggtgaa ggatgagatt tatttgtcgg
    14821 ctggccttga gtttagcggg gtatgtcagt actataacgc gtcatttttc agccgacctt
    14881 taacacgttc cttgcctccc cgggattcgg ctgacccaga caggaggctg aataatccgt
    14941 aaggagcaat tcgatgcgtc attacgaaat cgtttttatg gtccatcctg accagagcga
    15001 acaggttccg ggcatgatcg agcgctacac tgctgccatc actggtgcag aaggcaagat
    15061 ccaccgtctg gaagactggg gccgccgtca gctggcttac ccgatcaaca aactgcacaa
    15121 agcacactac gttctgatga acgttgaagc tccgcaggaa gtgatcgatg agctggaaac
    15181 taccttccgc ttcaacgatg ccgttatccg cagcatggtt atgcgtacca agcacgctgt
    15241 taccgaagca tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt
    15301 cgcaaacgaa accgctgatg atgctgaagc tggggattct gaagagtaat ttctgatgac
    15361 caaccgtctg gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg tcagtccatc
    15421 aggaattcct cactgccagt tcgtgcttga gcatcgttct gtgcaggagg aagccggctt
    15481 tcaccggcag gcatggtgcc aaatgcccgt tattgttagc ggacacgaaa accaggccat
    15541 tactcacagt ataacggtcg gcagtcgcat aaccgttcag gggttcattt catgccataa
    15601 ggcaaagaac ggactgagca aaatggtttt gcatgccgag cagattgaat tgatagattc
    15661 tggagactag ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg
    15721 aaggcgttca agagatcgac tataaagata tcgctacgct gaaaaactac atcaccgaaa
    15781 gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg
    15841 ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc gtacactgat cgccatcagt
    15901 aatcggtcac ggtccattaa tacgactttg agaggataag gtaatgcaag ttattctgct
    15961 tgataaagta gcaaacctgg gtagcctggg tgatcaggta aacgttaaag cgggctatgc
    16021 tcgtaacttc ctggtaccgc agggtaaagc tgttccagct accaagaaaa acattgaatt
    16081 cttcgaagca cgtcgcgctg aactggaagc taaactggct gaagttctgg cagcagctaa
    16141 tgctcgcgct gagaaaatca atgcactgga aactgttacc atcgcgtcta aagctggcga
    16201 cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg taactgcagc
    16261 tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg
    16321 cgaacacgaa gtgagcttcc aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt
    16381 agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg ccgaccagtg gtcggcgttt
    16441 tgcttttaag tgataaaaac cgcagaaaca gacgttccct ccataaatct cttgctattc
    16501 cttttataaa aagttcgcag tacccatacc tggcccccag cctcgccgct ggtagttcgt
    16561 gtcatgtttt ttacgcgtaa aacacgacaa aagcatcaat acagcgatcg tctcacttgt
    16621 atgataataa aaacaatatg ttatatatga atgtgctgtt attttattgt ttttatatat
    16681 aaaaagataa aataccatca ctttcgtaat cttgaacgct ttgcaggtgc gttcaatcca
    16741 ataaatgcta atattatggc tatcatagcc ataaaaagat aaaatattat cactatgggt
    16801 aaaaaaacag caccgttact cccttatacg aatcagatgc ttagtgaatt tggtgagcgg
    16861 ctgaaacttg ctcgtttacg acgcaggctt accgcaaagc aagttgctga acgtgctggg
    16921 atgtcgccaa taacattgcg ctctctggag tcaggaagtg caggggtcac gataggggca
    16981 tatttgtctg tgatgcaggt tttgggcctg gaaacggatt taaataagct tgcagctgaa
    17041 gacgacttag ggcgtcagct ccaggattcc cgtttgatta aaaaaagagg gctttctcgt
    17101 gcatcaagta agaatgaaga aggttctcag acagcctcat tgcagatgag gcacgagaaa
    17161 gaaggtcatc tcaccacgga tttaacagca ctggcagaag gctcgacgcc atctattaca
    17221 ggagaaacat caacctctct tgcagcacta ttaaaaccga taaaaaaacc gttaaaagga
    17281 aattaattat ggcattaatc ggagtgtatg cggattggga aggcctggat ggtccggagc
    17341 ggattggtta tttacacagt cgccgcactc ggacgcgtga gatatttgag tttgagtacg
    17401 ataaaaaggc gcttgcagat ccttcactta atttcattca gttagatcct gaaataatgt
    17461 tgtatgaggg agcacagtac ccaattcctc caaaggataa atttggtgca tttagtgatt
    17521 cctgcccgga tcgttggggg cgtatgctaa tgaagcgtcg ttttgagagg gatattcgag
    17581 acgggttatg tgataaagac tcccaccttt atgagtctga ttatctacta ggtgttcatg
    17641 atctttatcg ggttggggcc cttcgataca aacgtgaaga cgctggagag tttcttgata
    17701 atcgcattga tgttgcagcc ccaccattta ccgaaatagc gagtctggaa agggctagcc
    17761 gggcaattga agaagatcct gataataaag agctaatggg gcaaaaatgg ttaagaatgc
    17821 taatcgctcc ggggggatca ttaggggggg cacgtccgaa agccagtgtt gttgatgaag
    17881 ctgggcatct ctacatcgct aaatttccca gtgtaaaaga tgagtatgat gttggtggat
    17941 gggaaatggc cgttaatgca cttgctgttg gctgtggact gaatgttgca cctgctcagg
    18001 cgcataaatt tgccagcaat taccattgtt ttatggttcg tcgttttgac cgcactaatg
    18061 cgggaaggcg tctgcatttt gcttcggcta tgacactaac caggcatcag gatggtgaag
    18121 atgcctctac aggtgtgagt tatctggaat tagcagatgt tcttatcagg catggcgcgc
    18181 agactaatac tgatcttaag gaactctggt ccaggattgt ttttaatatt cttgtttcta
    18241 ataccgatga tcatttacgc aatcatggat ttatcttgat accaggaaaa ggttggcggc
    18301 tttctgaggc atatgatttg aatcctgtgg tcaggtctga tggtttaaaa ctcaatatta
    18361 cggaaaatga caatgcactt gatttggaat tagctagaga agttgcagag tattttcgac
    18421 ttagtttgac ggaagctgat gacattattg ctaattttag agggattgtc agtcagtggc
    18481 gaatcattgc cgagcgtctg agattacctg gtcgtgagca agagttaatg gcagaggcat
    18541 tcagagcgtc aatttagaat agttatttcg atatattcgc taaatcataa atttgcgaag
    18601 caatgccagt agatactgaa cgttttggaa gagacgttta atttatgaga aaaatagaaa
    18661 cgctacaaag ctaaattata gcgtttctat gtaaaataat tagcttgtcg ctaactgtac
    18721 ttgtggttct tcgtctttaa ctttgataag catccacagc gcaatggcgc caataatgtc
    18781 aaagaaagcc agggcaataa agaacggcgc gaagccaata atgtgaacga atgccccgat
    18841 aaacaggttg aaaatcaact gaccgctcca tgcacaggct cctgccatac ccacagcggt
    18901 agctacttcg tcttttttga ataggtcacc gcccagagtt gctgcaacag tagaaagaca
    18961 ttgatgcgag aatgcgccaa tgctaatcag taatacggcg atgtagggat tttcgacaat
    19021 actcaccaga ccaatcgtca tcataatgac ggctgcaata gtgaaggtaa ttcttcgtga
    19081 gttaattaaa ctcacgccgc gatcgtggaa gaacttcgcg acaaaaccac tggctaaaca
    19141 gccgagatcg cccagcagga aaggtagcca gacgaacatg gcaatttctt tcaggctaaa
    19201 atgcagcgtt tcgacgaaga aaatcggtac ccagaagtta atggttcccc atgccgggtc
    19261 agcgagaaaa cgcgcgatgc caatccccca gaaattacgt tggctgagga tatgcttgat
    19321 tgacgttttt tctttcttat ctgttttaag atgctgttct tgcccagact caatatagtg
    19381 gcgctcttca tcagaaaggc gtttagcatc ttttggatct ttataacaaa agaaccaaaa
    19441 taaagccgcg agtaaagcaa ggctacctga gacaataaac gcaaattgcc agctatgaaa
    19501 cataatgcac caggcaatca acggtggagc aagcatcgcg ccgagtgagg tgcccatatt
    19561 gaaaacacct gtcgccacgc cacgctcttt tgccgggaac catgttgatg cggtttttac
    19621 acccgcagga attgccgatg cttcgctgaa acccatcagg gcgcgcataa atgccagacc
    19681 tgaccatgtt cctgtcaagg catgccccat tgtcgccagg ccccagattg cggcacatat
    19741 cgcaaaactt aacttcagac ccacggtatc aataaagaag cccattatcg gttgccccaa
    19801 tgtataggcc aactggaatg cactgacaat ccatgaatat tgttctttgg ttattccggt
    19861 tgtctccata atcgttggag ccgcaatacc taacgccgtt ctgtccagat aattgattac
    19921 agtgataaag catacgaggg caatcatatg ccatcgataa ttctttattt tattcatttt
    19981 taaacctttt aattatatta ataaaccctg gttgttggta tttccatgta ataggcaata
    20041 aaaatcaggt acagaggaaa tattgaaaat tattgtgtgt aaatgtctcc ctttaaaaaa
    20101 tctggccagt ttttttcaaa gtaaataccg ttaatacacg gttgcatacg taaagacttt
    20161 acttcggtaa taaaagaacc ttgcgaatat tgaattgtta atttgtgatt tttgtcttca
    20221 gcgagttcag agcgaaaacg ccattcttct tttgcgccgc gacgagtttg tggtaggcat
    20281 ttgccttgtg gatcaataac catacgcgcc ccacggctac caccaccagc atcaatataa
    20341 tgcgttaatt gtgttaatac cgcggcagaa gtcagcgcca tatgccgcca cataaacagc
    20401 tcggcaactt cacccacatg tttgattgcc aatccatgcc gttggacaaa ttcgttcaat
    20461 agcagggcat cacgagtggc acgtcggact ttatcggcat ggcaaataaa tcccgcatag
    20521 tcagacattc gtgcctgaat tttttctctc acaaccgaca acggcatccc ggtcccgtta
    20581 tcgtgcgcct gagtaatgat ctctcttata gaagccagtg ccggagctac cagctgtgct
    20641 atatctccat caatattacg tttttgcgtg caaccaataa aacgtgccag acgaacagca
    20701 aaaacctgcc cggcattcaa tgccgcacca ccagggcgag tgacgccgtg tgtgccagca
    20761 acttccccca cggcgaaaca gccgggcagg gaggtttgtc cccagatatc cacttcaatg
    20821 ccgccattca tatgctgatt attcatggca aactgcaaag gctgtgtggt gagatcgtaa
    20881 ccgtgcatct tatacagcga gatagacagc ggattcattc gttgcagccg ttcgatgggc
    20941 gatggtgcca gagcgtcgtt attttccaga tacgcgcgaa cgtcgtcgtc cagtcgatct
    21001 aatgagaagg gcaggtcacc cggcaccgct tcaggattgc gattgaaatc cataaatacc
    21061 tgacgccctg attgctgctc ttgcgctaca gccatatcta acaagctgga gccaaactcc
    21121 attacccgag tggcgtggaa cggccactga tagcctttac gaaaaatgtt tgaagccagc
    21181 tcctgggtgg tgcgatagta atccgcgagg aagttatact cgttgccccc ggcatccacg
    21241 gaatagatat gtgggatcac ctgcacatag gtgccggaca aattccacgg aaacgtgctg
    21301 cgcggtgtgc ctataccaaa ctggctttca gtcaaattgg ttagcgtcag accttcttcc
    21361 agcgccagcc ccagcgagcc gaaacatttg tgtgggtaca cactgtcgcg ataaagctcg
    21421 ccaggccctc ctgttgccag taccacattg ggcgcaatca caattgccag cccccatggg
    21481 ttatggacgc gatgaccggt cgcgaggata gcccccgcca cgcggtcttc gccgttttcg
    21541 tcacgctgat gcagcagttt aatcactgtt gcactggtca acattgggat agcaagacgc
    21601 tgtacttctt ccagcagcac tttcaccatt aaccttgagg tacgtggccc acacgaggtt
    21661 gcacgcccgg cttcgtcatg gtcggtttga taacgaagaa tcgcaccata gcgatcttcc
    21721 ggtaattcca gaccaagata ttgcaacccg ccaagagtgt gcagagaacc taccgcttcg
    21781 acataagcag tgtcgtgatc catcgcccca ccactcgcca gtgcctctgc cagtttggtg
    21841 aagttgtcgc cgttgcccgc ggtagcggcg gtaaacagcg tctgtttatc ggaaccggag
    21901 caggccgatg ttcccatgta taacccggcg gtggcgatta acacattttg ctggcggcgt
    21961 ttaagttcca cagctgcacg cagcccggct gcaccactgc cgactaccag ggcatctgtt
    22021 tgccaaagtg ggtaatcaac accgtctacc gtgacaaccc atgcgggttc tgcctgtgca
    22081 aaaccctttg ggaaagtaac gtgcgtcagc gcatcaagga tggtgtcatc aagctgactc
    22141 atgcatcctc catcggcgta aacaggctac tttccatcag gcgtgggtgc ttgataattg
    22201 gacggaaagc catatacggc agaatgtcgc gctcaatctc cacacccggc gcgatttcaa
    22261 ttaattcaac gccttctgcc gttaactgga atactgcgcg ttcagtgata tacagcactt
    22321 cctgaccact ttgctgtgcg taggcggcgc taaaagtgat tttttccact tgggtaacca
    22381 gtttagggat ttgacctgac tgggcgatat gcaggccatc aggcgttaca tcaatcttgc
    22441 tgcctttggc gtcaaacgta ccgcagaaca ccactttacg ggcgttttgc gcaatttcga
    22501 ggaagccgcc ggggccaatg agattgccat tgagatggga tacgttgacg ttaccgtacc
    22561 gatccatctc tcccatgccg aggaaggtga tatcgatacc gccgccactg tagaattcga
    22621 attgatcgag tgatggaata atggcgtcgg cgttacgggc gcagccgaac agagcatcat
    22681 ccagcatcat gccgttatgt acgccttgct cgacgctcat ccataaactt tgataaggca
    22741 cgccttcgcg cagcgcaacc ccaggaatac cgcccggaat accaaagcca aagttggttg
    22801 aagcgcctgc caccagttcc cgtgctgccc gacgggcaat caggcgacgc accggatgac
    22861 taaccagttc aatagcgtcg ttagaactta gacggcgatg ttgaccgctg atcgtcaggt
    22921 cgtaaccgcc aagataggtt tgcggttgtt cgcggtgttc aacgatacca tcgaccagaa
    22981 tccccggtag cttgaccttg cgaggttcaa tggcgccagc ttccagtaca tcgcgcacct
    23041 gtacaaacac tttgccgcca ctgttgtgtg ctgccagcgc catcgaatag ctatccatat
    23101 caatcgcttc ttcttcgagg ctaacgttgc cacgcggatc ggcataggtg ccgcgcaaaa
    23161 tcgcgtagtc cactttgaaa gggcgatagc ggagtttggt ttcaccgtcg atttcaatca
    23221 gttctaccag gtcgtcggtc gtgcattcat tcgacttacc gccaccattg cgtggatcaa
    23281 caaacgatcc cagcccaacg tgggtaaaaa gccccggacg tccggcaccg atctcccgca
    23341 gcagtgcctg aattacgcca ccagggaaac agtaggcttc aatagcattg cttttcacca
    23401 gttcctgcat cttgggcgac caggtaaaat gtccggcaat aatgcgtttg agcatttcgg
    23461 cgtgagcaaa acggttagtg cctttgctgt cgcgatcgcc aatacccagc gagtggatta
    23521 atgtcagatc gcgcgggtgt ccggtttgca ggaaacgcgc ttcaatggcg gccataatat
    23581 ggtcagcttc caccataccg ccgccgttac cgctaatggc aatggttgcg ccgtcctgaa
    23641 tttgcgccgc cagtgtctcg gcggttgata acttacgcat acaatttcct tattgattgt
    23701 ggaagctcac gccggctttt tcggcaaatg cgtgcgtcgc ctgctggcaa tcctgagtgg
    23761 cggacattaa tccagccagt aactggtggg agtaatcggc gtgtttattt tccagcgcca
    23821 gaatcagttg cttaatatgt gccagtgcca ccggaccaca tttttccagc tgcgctaact
    23881 ggttcgccat ccagctttcc acctgttctt tttctaccac ttcatcaatt aaattcgcca
    23941 cctgggcttc ctgcgccgtc aggcgtttac ccagcatcag catctggcga ccgaccaccg
    24001 ggccaacctg attaagcacg cgttcaatgc ccatccagcc aggcaccatg ccgagcatca
    24061 cttccgggtt agagaatttc gcgccggggc gagcgatacg aatatcgcag cacaaggcaa
    24121 gttcaatacc gccaccgatg gtatgaccgt tgagattcgc aacggttaat tggggtaagt
    24181 tgcgcaggcg attaaaaacc tcgttaccgc gtttaatcca ttttctgccc atatctaatg
    24241 gcgaataggc cgaccacgat ttaatatcgc cgcccgcaca aaagaattta tcgccggttg
    24301 ccgttaattc gactaatcgt aatgtggtgt cgttttcaat ttcatttaaa taatggtcga
    24361 gagattcgat catttcttca ttaatagcat gacatttatc ttcacggttc aatgtcaggc
    24421 ggcaagaagc cgccgctctg ctgaacagaa cgggttggtc actcatgatt attccttagc
    24481 aataatgtgc gatgcgatcg acgctaatat ccagcgtgct gttaatatct ttctgccacc
    24541 agtacggtga gaatatttcc agttcaatag cgccattaaa tccggcgttt tcaaccagcc
    24601 gacgaattga cggaatattg atgacaccat ctcccggcat tccacggtca ttgaccagat
    24661 cggtggtcgg cactaaccag tcggaaacat gaaacgcgag gatgcgtttt ccggcacgca
    24721 ggatctgact ggcgagatcc gggtcccacc agacatggta gacatctacc gccacgccga
    24781 ggccaaattc gccatcggga tcgagttcat cacaccagtc cagcgcctga cgtaacgtgc
    24841 acagacaaga acgatcggca gcagtcatcg ggtgcagtgg ctccagtgca attggcacgc
    24901 cgacctcttt cgaatgtggc agcaactggc gaatcccttg tttcacctgt tcccgtgctt
    24961 cgtacagatc ttttgttccc attggcagac cgccaaccac ctgcatgtaa caggcggcat
    25021 ttagcacagc ggcatcgtcc agcgcccggc ggttatcatc aatcgccagc ttgcgctccg
    25081 ccagcgtcgg tgcggtgtag taggtgctac ggcacaaacc ggaaacgttt atgttactgg
    25141 cagcaagctg gcgggcgatt tgctggagat cttcaccctg caactccctg cgccaggggg
    25201 caatggcgcc aataccgcgt gcggcgcagg catcaataat ggcgggtagc ggcgttttat
    25261 agcccagtgt cgcggtattg atactgaata aatccgggcg ttgtaacaga tctctcatga
    25321 ttcagcctca gaagcgcggc atacttaagc cgccgtcgat ggcgaccgcc tgaccacagg
    25381 tgtaaatcag tttaccttcc gccatcgcgc ggacagtgga agcaatatca gccggataac
    25441 cccaacgtcc ccacggcacc agacctttgg caatcagttc gtcgtaatag gccgttgccg
    25501 gaatagtcat atcggttttg atgagaccag gacgcacttc atatacgcca atttgttcat
    25561 tacaaaggcg agcagcaaat agacgagcag cggcagagac ggcagttttg gcaatggtat
    25621 attcgccgcg attcatcgcc agcatgatgg cgttgatgga actgacgaaa ataatcgagc
    25681 ggtgcggcag ttctgcttcc ggtttcggct gtgccagtaa gcgcttactg aatgcctgag
    25741 cgaggaagaa cggtgcgcgg gtgttaatgg caatattttg gtcgaagctg tctggttcga
    25801 ggtcgagaag atcaccgcgt tttttcactg aaatgcctgc gttgttgagc aggcagtcca
    25861 gacgtcccca ctggttttgc gctgcgtcga gcatctcttc atgcagggag agatcgccca
    25921 catcagcagg gaagcaaatc acttccacgc cttccgccag acattcttgt tgtgtggcat
    25981 gtaatttttc tacgctatcc gcatccggac gatcattaat cagcagatta aatccgccac
    26041 gcgcaagctc cagcgcacag ccctttccaa taccacgagc ggctccggta atcacggcaa
    26101 caggacgagt ggatttcata caatagctct ctttttttga attttgtttc cttagtgatg
    26161 attagcttgc gactggaggg taaaaagatc aaccatctag ttggttgata tgcgatggcg
    26221 atcacaggac atttgataac ctgatgaaag tacaagtttt tgcccttttt tctgaagctg
    26281 aaatcaaatc aggttatgat agtcaccacg aagaggtcgt ggggaagcgt tatgtatctt
    26341 gataccagca acgtacagag cctgaaagaa aagctgctgt tatgcgcggt taatgaattt
    26401 gccgaatatg gttacgaagg ggccagagtc gataatatcg tcaaggccgc ggggtgcagt
    26461 aaacagacgg tttatcacca ctttggtaat aaagaaaatt tatttatcga agtactggaa
    26521 tatacctgga acgatattcg acaaaaagaa aaggcccttg atttctcaga tcttccgcca
    26581 cagaaagcca ttgagaaaat aatcgatttt acatgggatt attatatttc taatccctgg
    26641 tttttaaaaa ttgtgcatag tgaaaatcaa agtaagggtg tgcattatgc aaagtcacag
    26701 cgtcttttag aaattaacca tgcgcacttg cagttaatgg aatccttatt ggatgaaggg
    26761 aagaaaaaca atatttttaa gccagatatc gacccattgc aggtgaatat taatattgct
    26821 gcgcttgggg gatattattt gatcaaccag catacgcttg gtctggttta tcacatcagt
    26881 atggtttcac cccaggcact ggaagccaga cgtaaggtca tcaaagagac aatccttagc
    26941 tggcttttgg ttgacccttc atctaccgcg cgcgaataaa actgccatcc ggctggcggg
    27001 taaacaggac ctgttgatta ttccccgtat caatggttaa gcccgtcacc acgccgctgg
    27061 cgttctggcg gattttcacc atctgcccgt tttgcaaatt actcagtggc ttatccgcgc
    27121 cttctacctg cgccatggca tagacatcgg tagcgggcag tccgtggtcg cggaacagct
    27181 gcgccatcgt tttaccgggt tcgacacgat aggagcgcca ttgattatca attccgctat
    27241 ctggctggaa tggttgcgta tgggtggttt gcggctgctc ttctggctgg ccttcctgta
    27301 ttggttctgg agcgaccgga gccacctgat caggatcatt ttgtggcgtg actaattgcg
    27361 cccgcagctg ttcttctgtc ggtggttgtg attgcgactg aatatctaac tgcgcttcac
    27421 gcgtcaccac cggcgcgttg ggcgtatcat cagatggaag cagaaatccg actaccaata
    27481 caatggcggc aataatgatc ccccgacgat gcatcggcgg cagcgggtcc atgaagcgaa
    27541 aattgtccgg cgcgtgccag actttctcca gggttggttt tagttcaaag cgcccgggca
    27601 tggtttcctc ctgctccgcg tcttgttcct caatcatagc ctatgaataa gctaacgcta
    27661 tgatgtccgt ggtaaacccg cctttattat attcatacgg gatattgcta ttgtttcttt
    27721 ttccccggga tttgtcatca ttcccgcgac aaagttttac ccaaagaagt gtggctgata
    27781 tgctgcccgc tactttatac cctaagaaag gaaatacgat gaccacccca acttttgaca
    27841 ccatcgaagc gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat
    27901 ctggcctgga agggctgctg ccagaagcac tggttgcagg cattgccgat gcgctggaag
    27961 gcaaacatcc ggctgttccg gttgatgtgg tgcaccgcgc gctgcgtgaa atccacgaac
    28021 gcgccgatgc cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc
    28081 tggaagaaaa cgccaaaaaa gaaggtgtaa atagcaccga atctggcctg caattccgcg
    28141 tgatcaacca gggcgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca
    28201 ccggaaaact gatcgacggc accgtgtttg acagctccgt tgctcgcggc gagccagctg
    28261 aattcccggt taacggcgtg atccctggct ggattgaagc actgactctg atgccggtag
    28321 gttctaaatg ggaactgact atcccgcagg aactggcata tggcgagcgt ggcgcaggcg
    28381 cgtccatccc tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag
    28441 cagcgcattc tgttcccctc gaaccagagg ggagcagaca ttcagcaata aacccttcag
    28501 tttgccaaac ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct
    28561 gtttcgcgtt atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta
    28621 aatcttagta ttcataccgc gtattgttac ctaatatcga tgagtcccga tacagattcg
    28681 tcgtatcata gactgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga
    28741 aaaaaacatg gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc
    28801 gctacggcgc aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg
    28861 tacagggttg tttatggggt ccggcaaaac gattagcctt gccgggccgt cgatcatttt
    28921 cgtttatatg atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct
    28981 gctttcgaat ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg
    29041 ggcaggatat ttcaccggct ggacctactg gttctgctgg gttgtaaccg gtatggcaga
    29101 cgtggtggcg atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc
    29161 ctcgctggcg gtgatagtgc tgctgctgac gcttaacctc gccaccgtga aaatgttcgg
    29221 tgagatggag ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgatagtcgt
    29281 cggcctggtc atggtggcga tgcactttca gtcaccgacc ggtgtggaag cgtcattcgc
    29341 gcatttgtgg aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt
    29401 ccagatagcg gttttcgctt tcgtggggat tgagctggta ggtacaacag ctgcggaaac
    29461 caaagatcca gagaaatcac tgccacgcgc gattaactcc attccgatcc gtatcattat
    29521 gttctacgtc ttcgcgctga ttgtgattat gtccgtgacg ccgtggagtt cggtagtccc
    29581 ggagaaaagc ccgttcgttg aactgtttgt gttggtaggt ttgcctgcgg ctgccagcgt
    29641 gatcaacttt gtggtgctga cctctgcggc gtcttccgct aacagcggtg tcttctctac
    29701 cagccgtatg ctgtttggtc tggcccagga aggtgtggca ccgaaagcgt tcgctaaact
    29761 ctctaagcgc gcagtacccg cgaaagggct gaccttctct tgtatctgtc tgctcggcgg
    29821 cgtggtgatg ttgtatgtga atcccagcgt gattggcgcg ttcacgatga ttacaaccgt
    29881 ttccgcgatt ctgtttatgt ttgtctggac gattatcctt tgctcgtacc tggtgtatcg
    29941 caaacagcgt cctcatctgc atgagaagtc gatctacaag atgccactcg gcaagctgat
    30001 gtgctgggta tgtatggcgt tctttgtgtt tgttctggtg ttgttgacac tggaagatga
    30061 cacccgccag gcgctgctgg ttaccccgct gtggtttatc gcgctggggc tgggctggct
    30121 gtttattggt aagaagcggg ctgctgaact gcggaaataa tcgcattatc atgctggatg
    30181 gcgcaatgcc atccagcttt tagatcactc acccgccagc gcgcgcggga atagtacatt
    30241 gttttccaga ctgatgtgct ccatcaggtc atcaatcagt tcattaatgc cgttatacat
    30301 cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg tgtgtttgat
    30361 cacttccagc agttcgcccg cttcatcgtg ctcgctttcc attacgctga ttggccccat
    30421 tgcctggctg cccatgcctt gtttaatcat cgggaagaga atttgctctt ctttcatcat
    30481 gtggctggaa agctcttcat gcagcatagt caggtatttt gtcagccctt ttggcacact
    30541 tggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct ccggcagttg
    30601 ctcgcggtgg cgatcgtggt agcgcacgat gatatggtcg atgatttctg ccagcggggc
    30661 gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcgg cttcaatgac
    30721 gtcaacatcc agttctttac gtgccgccgc gcgcgccagc gtctgcttac cgccacagca
    30781 gtaatccata tcatatttac gaaacagagc cgaagcgcga ggaatagaga gcgccagttc
    30841 acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa gataagatgt
    30901 attttaaatg catctttatg gcaaatgcta taacccttac gtagcagagg cttatttaac
    30961 tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc tggttttgct
    31021 ctgactgcaa tgaccacgcc aaccactaac agcgcgatac cgcttagcgt cattagcggc
    31081 ggcatttgct ggcgtaacaa aaaggtgtac agcaagcctg ccagcgtttc gaaaacaatc
    31141 agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca gagtgcgcca
    31201 acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag cggacggggg
    31261 ccaaagggta gggagaagcc cggcgtttgc gtattcagcc agtaacaggc gacgagatag
    31321 ccgatgagag aaaccggcag cgtgaccagc gcctgcgccg tcgcccacat catcggatgt
    31381 ttgtcgggat tctcccgcag ccagcgggcg ttgcgcaggg cataccatgc ccagcagacc
    31441 acggaaacta acgccagcac gatgccagag gtataacgtg cccagtcaaa atcggggagt
    31501 ccgtggttta actcagcaat attcacacac gccaggccga tgccaataca aaccagtgcc
    31561 ggggcgagtt ttccccacgc gagtttgccg tcgcgctggc tataaagcag attggcaaaa
    31621 acgggaatca ccaccggcag ggtgccaata atcatcgtgg aaacaggcgc gccagtacgt
    31681 tgaatggcac tggcaaggca gaagtaatag atgaggttgc ccatcatggt gagcatcaag
    31741 gcggtaagcc agtcgcgacg cgccaactga cgcagacgca cgcgtcccag ccaggcaatg
    31801 ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat cgccgggtat
    31861 tccggcacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc taacagggcg
    31921 tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa gttcaggaag
    31981 cagacatgat tgctcccggc atcccgtcat tatcagagag gatgcgggag gatgccactt
    32041 taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac gttgtcgttg
    32101 ggcggccaat caatttgcta agcgttttgc tgtcatcaaa cagaccgcct ttcgatgcgc
    32161 caacgtcaga atccgccagc atatccgcca gtccatcagg cagtccgacg cttttcagtg
    32221 ccgcagcgaa atcagcttcg ctcaggtttt gataggtaac ctgtttgccg ctctgtttgg
    32281 tgagttctgc cgctaactgt gtcagcgtcc aggcactatc gcccgccagt tcgtaaacct
    32341 tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc taccgcataa tcagcccgcg
    32401 ttgctgaggc aattttgcca tcgcctgcgg caccgataaa tacgccgtgc tccagtgctg
    32461 ccggggcgct ggcgaggtag ttttcggtgt accagccgtt gcgcagcagg gtgtaaacga
    32521 tgccagaatc agccagcatt ttctccgtct cgatgtgctc atcggcgagg ccgagcgggg
    32581 aggaatcggc atgtagcagg ctggtataag cgataaattt cacgccggcc gcctttgcgg
    32641 cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa gagatcagca
    32701 gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg taatcagcct
    32761 gacgcacggt aatgccttgt gctgtcagtg cctgtgcttt tgccggatta cgaacgatag
    32821 ccactatttg gctggcagga accgttttca tcaaggattc aataacatag tgaccaagtt
    32881 ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc ttgtgtttaa
    32941 atacgctaac acttaaactt acttttagta agtacgtaca aaaaggtaag tatgagatga
    33001 gtcaggttag cctgtcgcaa caactgaaag agggaaatct cttcgctgaa cagtgtccgt
    33061 cgcgcgaggt tttgaaacac gtcaccagcc gttggggggt gttgattctg gtggcgctac
    33121 gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat gggcggggtg agtgaaaaga
    33181 tgcttgcgca gtcgttacag gcattagaac aggatggttt tcttaaccgt atcgcgtatc
    33241 cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag gtgagcgaaa
    33301 aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgtta gcggtgcggg
    33361 atgaacgtgc ggcataactg ccggatgcgc tgcgcttatc cggactacgg ttcatggctt
    33421 gtagggcgga taagatgcgt catcatcgca tccgccaata aatgccggat gcggttgatg
    33481 atttgtaggc cgaaaaagaa gtgttttact tactcagatc cacctgataa atcgcaaacc
    33541 cgatatcatc ggtcgcgact ttattcatcg gatactgccc tttctcttta ataaacactg
    33601 cggctttatc tgacggagag gtttcgaaac ggatatccag tttcttatcg ccagctattg
    33661 gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt ttcgactcat
    33721 cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa gcgatatggc
    33781 tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg gcaacgagga
    33841 acatggcgtt cggatcaatc ggcttgccgt taaaggtcag gttcttaatc ctttccgcat
    33901 tggcattaat catctggcac tcgccgtcat aacgggcggg ctgggtaaca tcaatctgat
    33961 aattcacacc atcaataaca tcaaagttat aggtgcggaa accatcccag ttgatgagtg
    34021 actgtggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag cactccagcc
    34081 actctttcac ctctttaccg ctggctttca ccacaatcag cgtattgggg tagagataaa
    34141 gatcggcggc attacggaag gtcaactggc ctttttccac ctccacatag cttgccgggt
    34201 catttttgcg accaccaact ttaaacggtg cggcagctga aagcactggc agttttgcca
    34261 gatccggatc gccctgaatg taatgctcga catacgcttt ttgcgcgttg ttcaccacct
    34321 gcacggtcgg atcgtcctgc acaagcgcca gatagctata catattgtcg gcagatttac
    34381 cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt gtttctacca
    34441 gcttgctgtc ttccgccgcg agggattttt tattcgcgat gtcgtaaatc ggccgtgctt
    34501 ccgctttcgc ctgcgtcacc tgccatttac cgctgtcatt actgagttgt aagtcgacca
    34561 caccaagatg atcgccccac atgcctggca ttaccgccgg aacaccattc agcgtgcctt
    34621 tcgcgatatc agccccttcg atatcagcaa aatctttacc tgggaaaacg gcgtgagcat
    34681 ggccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact gagttttccg
    34741 ccatcacttt atacggatcg gcagacagcc cggaatgcgc cagaacgaca acgacatcgg
    34801 cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata tcattcaccg
    34861 tcactttccc ggataaatta gctttatccc agcccatgat ttgtggcggg acgacgccaa
    34921 tataaccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg gtgtctttaa
    34981 ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg gcatttacat
    35041 aagggaattt cgctcccgcc agcgcatttt tcagataatc cagaccgtag ttaaactcgt
    35101 ggttgccaag cgtgccgacg gtatagtcca gcgtatttaa tgccttatag accgggtgaa
    35161 tatcacctgc ttttaaacct ttcgccgaca tgtaatctgc aagcggactc ccctgaatca
    35221 aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg ttaatcaggc
    35281 ttgcggtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa tcgaaatcca
    35341 tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc gctgcattca
    35401 cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc agggacatcc
    35461 ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat agaacagtga
    35521 agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc gcattcatta
    35581 acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag gtggagaaat
    35641 gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc aggtctacga
    35701 cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa cggcagcgga
    35761 tattgccgca cacaccgtta tcatggacgg tttacgtacg ctgacaccgg atattccggt
    35821 gctttctgaa gaagatcctc ccggttggga agtccgccag cactggcagc gttactggct
    35881 ggtagaccca ctggatggta ctaaagagtt tattaaacgt aatggcgaat tcaccgttaa
    35941 cattgcgctc atcgaccacg gcaaaccgat tttaggtgtg gtgtatgcgc cggtaatgaa
    36001 cgtaatgtac agcgcggcag aaggcaaagc gtggaaagaa gagtgcggcg tgcgcaagca
    36061 gattcaggtc cgtgatgcgc gcccgccact ggtcgttatt agccgatccc atgcagatgc
    36121 agagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca tcggctcttc
    36181 gctgaaattc tgcctggtgg cggaagggca ggcgcagctg tacccgcgct tcggaccaac
    36241 gaatatttgg gacaccgccg ctggtcatgc cgtggctgta gctgccggag cgcacgttca
    36301 cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga atccggggtt
    36361 cagagtatct atttattaat taatttatgc agcaggtcca tcacttgctg cacctcttcc
    36421 tgagtaagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag caccactaca
    36481 gcggaacttt cttcatccag ctgccaggca ccgcgcgcga cgccattgct atcgacaata
    36541 aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac aaacatgccg
    36601 gaacccggaa ttgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg atcgtgcggt
    36661 aactttgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc agaggtgcga
    36721 ccagcaatat gttgcagtac tcgcactttt cccactagct gcgcgctgtt ccaggttttg
    36781 tagctaaact gatctttatc aagcaccaac tcgccacgat cggtaatgcc aatcggcggc
    36841 actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag caggcaggtg
    36901 agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca ggtgatccga
    36961 ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc aagtgtgatg
    37021 ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact gaagacggcg
    37081 cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag ttatacgcgg
    37141 tggaaacatt gcccggatag tctatagtca ctaagcatta aaatttgcgc ctcataatag
    37201 ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca atgaaaattt
    37261 tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc cgtggacggt
    37321 tatacatcaa ggacgttggc gcttttgaat ttgataaggg taagattctt atcccaaaag
    37381 tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt atgcgtctgc
    37441 aaacagagat ggcttaacca aagtgctatg cagtaataag acggctcctg attcaggagc
    37501 cgttgatgtt tctgggggtt acgcgacgct ttcttcttta tctttagcgt caggcaattt
    37561 tggcgaaagg gcggtggcct tgctgtcgat tcgggtcacc agcagctggt cgatgcggta
    37621 gttatcgata tccaccactt caaatttgta gccggcgaat ttcaccgaat cggtgcgttt
    37681 cgggatctta cgcagcataa acatcataaa gccgccgatg gtttcgtagt tgcccgactg
    37741 cgggaactcg tcaatatcca gcacgcgcat gacgtcgtca ataggcgtgc cgccgtctat
    37801 cagccatgag ttctcatcac gggcgacaat ttgctcttcc agcccctgac cgaccagatc
    37861 gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc cccactacca gcgcgtactc
    37921 gttcatgatc accgcgaagt cttcacctgc ggttttaaaa ctttccaacg cctctgaaag
    37981 ggttaacgta tccggcacaa tcagcgtatt gcgaatttgt acgccgctgt tcagtgccag
    38041 gctttggtta gccagcacgc ggttcagcag gtctttagaa tcgacatagc cgatgatgtg
    38101 gtcaatatct tcattacaga cgagaaactt agagtgcgga tgttccgcca ccttattctt
    38161 cagactttgc tcatcttcgt ggagatcaaa ccaaatcacg ttttcacgcg gtgtcattga
    38221 agacggaacg gtacgggatt ccagctcaaa gacgttttca atcagctcgt gttcctgttt
    38281 acgtaacacg cccgccagcg caccggcttc cactaccgcg tagatgtcat cagaagtgat
    38341 atcatcttta cgtaccattg gcagtttgaa aatacggaag attatgttcg ccagtccgtt
    38401 gaagaaccac accagcgggg tgcaaacgta caggcagaag cgcatcgggt tgatgatacg
    38461 caaagccaca gcttctggcg caatcatacc gatgcgtttc ggggttaaat ccgcaaacag
    38521 gataaacatg ccagtcacta acgagaaaga gagaataaag ctcagttgct cagagagttc
    38581 tgccgacata tagcgggaga acaggctgtg aaaagctgga gaaaatgccg catcaccgac
    38641 gataccgccg agaatcgcca ctgcgttcag accgatctgg accacggtaa agaacatgcc
    38701 gggattttcc tgcatattca gaacgcgttg ggcatttata ttgccttcat cagccagcag
    38761 tttaagtttg attttgcgtg aggcggcaag cgagatctcg gacatcgaga agaacgcact
    38821 tacagcgatc aaacagagta tgactaaaat actgtttaac atatcttatc cgacctttca
    38881 ggccagatcc tcggaagggg aagttgatta atttgtgtga aatacacatt gaaagccgat
    38941 tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc gtaaggtact gtaaagccgc
    39001 cagagggtta aaattcagac agctgaaaaa tgaaaaactg cctgatgcgc tatgccgaac
    39061 aggcctacgc agtccccgca atatattgaa tttggacgat tttgtaggcc tggcaaggcg
    39121 tttacgccgt atccggcata aacaaaatgc actttatcaa caatctacat ttgtacccgt
    39181 aacgctatgc ttccggcggc agacagacgc caattccgcc aattccacag taaccatacg
    39241 ggtttttatg cagatattgc tggtggtcat cttcggcata ataaaacggt gtggcgttag
    39301 cgatttccgt ggtgatgcga cgatcgtcat cggcggcaag catcgccgcc tgaaaacgtt
    39361 ccagactggc gcgagcggcg gcatcctgtt ctggggtcag cggataaatc gctgaacgat
    39421 actgcgtgcc gtggtcattg ccctgacgca ttccctgggc gggatcgtga ttctcccaaa
    39481 atacttgtag caactgctca tagctgatga cggatggatc gtaaacaatg cgcaccgctt
    39541 cggcatggcc agtatcgccg gagcacactt cccgataagt cggatttggc gtatagccgc
    39601 cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg ccagaacaga cgctccacac
    39661 cccagaaaca acccatcgca aaaatggcaa tctccattcc gtcaggtaca ttggtcattg
    39721 agtgaccgtt gaccgcatgc agtgtggcta cgggcatcgg ggtgttacgt ccaggcaggg
    39781 catcggcggg ggaaaccaga tgctttttat caaataaact catggtgtcg ctctcccgaa
    39841 aatcggtcat tggggttaag gttgtaacaa gaggcgtatt tgcacacaat aaccaccgtg
    39901 aataggtcta aagtaaaaca taagaaatat tttgggttta gtctgctttt taatccatat
    39961 tactggattt ttgttaagcc gtttaacggc gttccagggg caggaaaaaa ggatattcag
    40021 gagaaaatgt gcgctatatc cgacagttat gctgtgtaag cttactctgc ttaagcggat
    40081 ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt atcgggacag ctggaaaaga
    40141 acgttcgcgc gcagctttct acgattgaaa gtgatgaagt gacgccagac cgtcgctttc
    40201 gcgcacgcgt cgatgatgcc atccgcgaag gtctgaaagc gctgggctat taccagccga
    40261 ccattgaatt tgatctccgt ccaccgccaa agaaagggcg gcaggtattg atcgccaaag
    40321 tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt ggtattgcgc ggcggcgcgc
    40381 ggaccgataa agactatttg aaattgctcg atactcgccc ggctattggt acggtactga
    40441 accagggcga ttatgaaaat ttcaaaaagt ccttaaccag cattgcgttg cgtaaaggtt
    40501 atttcgatag cgaatttacc aaagcgcagc tgggcattgc gctcggcctg cataaagcct
    40561 tctgggatat tgattataac agtggcgaac gttaccgctt tgggcatgtg acctttgaag
    40621 gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc gtttaaagag ggcgatgagt
    40681 acgaatcgaa agatctggca gaactgaacc gtcgactttc tgctaccggc tggtttaact
    40741 cggtggtggt ggctccacaa tttgataaag cgcgcgaaac gaaagtatta ccattgacgg
    40801 gcgtggtttc gccgcgaaca gaaaacacca tcgaaaccgg cgtcggttac tctacggacg
    40861 tgggaccgcg cgtgaaagcg acgtggaaaa agccgtggat gaactcttac ggtcacagtt
    40921 tgaccaccag taccagtatt tccgcgccgg aacagatcct cgacttcagc tataaaatgc
    40981 cgctgctgaa gaatccgctg gaacaatatt atttggtgca gggcggtttt aagcgcactg
    41041 acctgaacga taccgagtct gactccacta cgctggtggc ttctcgctac tgggatctct
    41101 ccagcggctg gcagcgtgcc attaacctgc gctggagttt cgaccacttt acccagggtg
    41161 aaattaccaa caccacgatg ctgttttatc ctggggtgat gattagccgc acgcgttctc
    41221 gtggtggcct gatgccaacc tggggcgact cgcaacgcta ctctatcgac tactccaaca
    41281 cggcctgggg ctcggatgtt gatttctccg ttttccaggc gcagaacgtc tggatccgca
    41341 cactgtacga tcgccatcgt tttgtgacac gcggcacgct gggctggatt gaaacgggtg
    41401 atttcgacaa agtaccgccg gatctgcgtt tcttcgccgg gggcgatcgc agtattcgcg
    41461 gctacaaata caaatctatc gccccgaaat acgccaacgg tgacctgaaa ggggcctcga
    41521 agctgataac cggatcgctg gaataccagt acaacgtgac cggaaaatgg tggggcgcgg
    41581 tgtttgtcga tagcggcgaa gcggtaagcg atattcgccg cagcgacttt aaaaccggta
    41641 ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat caaactcgat tttgccgtac
    41701 cggtcgcgga taaagacgaa cacgggttac agttttacat cggtctgggg ccagaattat
    41761 gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt atcttactgt tgctgggatc
    41821 ggtggcgttt ctggtgggca ccaccagcgg cctgcatctg gtatttaaag cggcggatcg
    41881 ctgggtgcca ggactggata ttggcaaggt caccggcggc tggcgtgatc tcaccttgtc
    41941 tgacgttcgt tatgagcagc caggcgtggc ggtaaaagcg ggcaatctgc atctggcggt
    42001 cgggcttgag tgcctgtgga acagtagcgt ttgtattaat gatctggcgc tgaaagacat
    42061 tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa caggttgaag aagaggaaga
    42121 tagcggtccg ttggatctct ccatgccgta tcccattacc ctgacacggg tggcgctgga
    42181 caacgtcaac atcaagattg atgacaccac ggtatcggtg atggacttca cctccggcct
    42241 gaactggcag gagaaaaccc tgaccctgaa accgacatcg ctgaaaggcc tgttgattgc
    42301 tttgccgaaa gtggcggaag cggcgcagga agaagtggtc gaaccgaaaa ttgaaaatcc
    42361 acagccggag gaaaagccgc tcggcgaaac gctgaaagat ctcttttctc gcccggtatt
    42421 gccggaaatg accgacgtgc atctgccgct caacctgaac attgaagagt ttaagggcga
    42481 gcaactgcgc gtgacgggcg acacggacat caccgtgcgc accatgctgc tgaaagtgag
    42541 cagtatcgac ggcaatacca aactggacgc gctggatatc gactccaacc aggggatcgt
    42601 caacgccagc ggcacggcgc agctgtcaga caactggccg gtggatatca ccctcaacag
    42661 cacactgaac gtggagccgt tgaaaggtga aaaggtgaag ctgaaagtgg gcggtgcgct
    42721 gcgcgaacag ctggagatag gcgttaacct ttccggtccg gtggatatgg atttacgcgc
    42781 ccagacgcga ctggcggaag ccggattgcc gctcaacgtg gaagtgaaca gcaaacagct
    42841 ttactggccg ttcactggtg agaagcagta tcaggcggat gatctgaaac tgaaacttac
    42901 cggcaaaatg accgattatg cgatctctat gcgcacggct gttaagggcc aggagatccc
    42961 gccagccacc attacccttg atgccaaagg taatgaacag caggtcaatc tcgacaaact
    43021 taccgtcgcg gcgctggaag ggaaaacgga acttaaggcg ttgctcgact ggcagcaggc
    43081 cattagttgg cgcggtgagc taacgcttaa cggtattaac accgccaaag agatcccgga
    43141 gtggccgtcg aaactcaatg gcttgattaa aacccgcggt agcctgtacg gcggcacctg
    43201 gcagatggag gtgccagaac tgaagctgac cggtaacgtc aaacagaaca aagtgaacgt
    43261 tgacggcacg ctgaaaggca acagttatat gcagtggatg atcccagggc tgcatctgga
    43321 actggggcca aacagtgccg aagtgaaagg cgagctgggg gtaaaagatc tcaatcttga
    43381 tgccaccatc aacgcgccgg ggctggataa cgcgctgccg gggcttggcg gtacagcgaa
    43441 agggttggtg aaagtacgcg gcacggtgga agcgccacaa ctactggcag atatcaccgc
    43501 gcgcggcctg cgctggcagg aactttccgt ggcgcaggtt cgcgtggaag gcgatatcaa
    43561 atccaccgac cagattgcgg gtaaactcga cgtacgcgtt gagcaaattt cgcagccgga
    43621 tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc gaaaagcagc acgagctaca
    43681 gttgcggatt cagggcgagc ctgtctccgg gcagcttaat ctggcaggaa gttttgatcg
    43741 caaagaagaa cgctggaagg gaactcttag caatacccgc ttccagacgc cggtcggccc
    43801 gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat aaggagcaaa aaatcagcat
    43861 cgggccacac tgctggctta acccgaatgc ggaactgtgc gtgccgcaaa ctatcgatgc
    43921 gggtgccgaa gggcgtgcgg tggtgaatct caaccgcttc gacctggcca tgctgaaacc
    43981 atttatgcca gaaaccactc aggccagcgg tatcttcacg ggtaaagcgg acgttgcctg
    44041 ggacaccacg aaagaggggc tgccgcaggg cagtatcacc ctttcggggc gtaatgtgca
    44101 ggtgacgcaa accgtcaacg atgcggcgct gcccgtggcg tttcagacgc tgaatctgac
    44161 ggcggaattg cgtaacaacc gtgccgaatt gggctggacc atccgcctga ccaataacgg
    44221 ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc cgccgtaatc ttggtggcaa
    44281 cgtcaatatc cgtaacttca accttgcgat gataaacccc atctttaccc gtggggaaaa
    44341 agcagcgggg atggtgagtg ccaacttgcg tctgggtggt gatgtgcaaa gcccgcagtt
    44401 gtttggtcag cttcaggtta cgggtgtgga tatcgacggc aactttatgc cgtttgatat
    44461 gcaaccgagc cagcttgcgg tcaactttaa cggtatgcgc tcgacgcttg ccggtacagt
    44521 acggacccag cagggtgaaa tctacctgaa cggtgatgcc gactggagcc agattgaaaa
    44581 ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg atcaccgtgc cgccgatggt
    44641 acgaatggat gtatcgccag atgttgtatt cgaggctaca ccaaacctgt ttaccctcga
    44701 tggtcgcgtg gatgtcccgt gggcgcgcat cgtggtgcat gagctgccgg aaagcgcggt
    44761 gggcgtctcc agcgatgtgg taatgcttaa cgataacctg caaccggaag aggcaaaaac
    44821 ggcgtcgatt ccgattaaca gcaacctgat tgtccacgtc ggcaacaatg tgcgcattga
    44881 cgcctttggc ctgaaagcgc ggctgacggg cgatctcaac gtcgttcagg acaaacaagg
    44941 gctgggcctg aacgggcaga tcaacatccc tgaagggcgc ttccatgcct atggtcagga
    45001 tctgattgtg cgtaagggtg agttactgtt ctctggtccg ccggatcaac cgtatcttaa
    45061 tatcgaagct attcgtaacc cggatgctac agaagacgac gtaatcgccg gagttcgcgt
    45121 cactggtctg gcggacgaac cgaaagcgga gatcttctct gacccggcca tgtcgcaaca
    45181 ggctgccttg tcttatttgc tacgtggaca agggctggag agcgatcaga gcgacagtgc
    45241 ggcgatgacc tcgatgctga ttggtctggg ggttgcacaa agtggtcaga ttgtgggtaa
    45301 aatcggcgag acgtttggcg taagcaattt agcgctcgac acccagggag taggcgactc
    45361 ctcccaggtg gtggtcagcg gctatgtatt gccaggtctg caagtgaaat acggcgtggg
    45421 tatatttgac tctatagcaa cactcacgtt acgttatcgc ctgatgccta agctatatct
    45481 ggaagccgtg tctggtgtag accaggcact ggatttgctc tatcagttcg agttttagca
    45541 atgcgaatat ttgtctacgg cagtttacgc cacaaacaag gcaacagtca ctggatgacc
    45601 aatgcccagt tactgggcga tttcagtatc gataactacc agttgtatag cctgggccac
    45661 tatccaggcg cagttccggg gaacggaaca gtacacggtg aagtttatcg tattgataac
    45721 gccacgctgg ccgaacttga tgccttgcgc accaggggcg gtgaatacgc gcgccagttg
    45781 attcagacgc cgtacgggag tgcatggatg tacgtttatc aacgacccgt cgatggatta
    45841 aagctaattg aaagcggcga ctggttagac agggataagt aataataaaa gcacgccacc
    45901 ttcgggtggc ataagggggt agcgcgtatc gctatccccc tttatatatc aaatggttac
    45961 gagttcaaag tggttcggaa tagtgccatt tagtccccgt ttatgggcaa aatctggaca
    46021 actccgcaac cggattgaac cttaccgcgt cctggagata atcaggtgca aagtgcgcat
    46081 aggtcattgt ctgctgaatt gtgctgtgcc ctaaaatacg ctgaagggta ataatgttcc
    46141 ctccattaat catgaaatga gttgcgaatg tgtgccgtaa aacatgcaca gactgaccga
    46201 ctggtaaatc aggccgcaca gtccttattg cacttctgac tatcgcataa ctggggttca
    46261 ttaatcttcc cgtcgctttg actttaatca gagattcaag agctttagct ataggaataa
    46321 cccgccgcga tccattcttt gtctccatga aaataacctt cccaccaatt atgtgttcac
    46381 ctttaatatt ggcaacttca ccccagcgtc cgcctgtagc caggcttaac aatacagcct
    46441 taagattatc gccagataaa agtgcggtta gcctgggaat atcatcatgt gcaagataag
    46501 ccatttccgg ctgattaatt gttagcttgc gtatctcgtg aaacgggtta tcacccacaa
    46561 actcttcaac atcaatcagc ttttcaaaga aaccgctcat cattgcacaa taacgattta
    46621 ccgttgactg cttaattccg cgattcatta attcaaggcg atatctgata atattttttc
    46681 gggttaagtg gtcacttctg ttaataccaa gctcttgcat atcacgaaga accgcttcaa
    46741 ttctgacccg attcatcgcg ccataacgat gattcttacc gtggtacacc caccacagtt
    46801 ccagaagatc gcttaacgct ttacgttcaa taggtttttc gacccagtcc ttatcgtgat
    46861 aatgcgtcag aacatggcgt tcatacatcg ccgcttcacc acgggtagga aatcgccgcc
    46921 gaaatcttct tccttcagat ccagcaggcc gcacgtccac acagtatttg ccatcagcga
    46981 gcttcttaat cgacataagg aagcccccca atgaaaacat catcttgatc acaattttga
    47041 gaaataaagg ccagatggac cgttaaccag tcttcttgcc ggagtgggat gattccgctt
    47101 cgctttgccc aatgtgtgcg agagccggta ccacctgccc ggcctttggt tcgacctcat
    47161 cgaacaaaaa ccagtcacgg tatttacgga aacgagcgtg ccgcaaaaga aggatcccaa
    47221 aatcaagagg catcttagat ttcccacttt catagccatg atatgtatag taattaattc
    47281 caattaaatc agatagttcc ttcattttca tccgttcgga ctctctgata agttttaatt
    47341 tctcattttg cggtattgac ataatttttc taatctccaa taatttttgt gtatagcaac
    47401 acaagaatag tcgcaattgg ttctacatgg tccctattcg aaaaaggggc caataacgga
    47461 ggataccaga tgcaagaagt cactcaaaac gcgcctgaat cgcctgccga aacacagtgc
    47521 gatcagttga cagatgcgcc agaaaccgca caccaaaaga ccgggcgcaa agcccctgaa
    47581 cgccaggaaa tcaggctttc acaaaaccct tctcatctcc tgtcaaaaga agggtttgcg
    47641 atgtatatcg gcaagaccgt tgacgccatc gtgagcatgg caaaagcagg gaaattaccc
    47701 gctgtgtaca tggcggatcc cctcaagcct ggcggaaacg ccgaacttta catcaaccgg
    47761 aaagcatggg atgaagcctg cgatcgactc actgaaaacg caccgcaaga atggcacgaa
    47821 tgggaaaacc gacttttttt atttaagcca gcagctggtt cacggaataa acgcatcagt
    47881 aaaaaagctg cgtgatatag aactggcaat cattgggagg acaggatgat taagaaatac
    47941 agcgaacacg ggagcaaggc tggcgaagca atcgccattg acgaggattg cctgataaca
    48001 ggagacccct ggctggcaac agaaagggat ttagaaggca aaacattttt tgtcagagca
    48061 ataaagcaca aagatagggt tgtgggtgga attatttatt caaccagtac gattattcct
    48121 gataattatc tggatatttc ttttagacct gtaagcattc aggggtgcga ggcatacctt
    48181 aattacgttg cggagctaaa gaaaattaac ctggactaca tgtggccaat cgtacaacgc
    48241 gaaataatgc gacgaaaatc agaaggcttc gatgtgaaaa ttgagttttt atcgacgtta
    48301 atgggatttt tacaaaaacc attgattctt ttcccctccc ttgaccggaa cctgccagtc
    48361 aaagaagggg tggaaaaata tattgttgaa caagtcagag atctggtttg tctttagagg
    48421 cgcgaataaa gcacgcaaga tgattaatac tatgaaatcg tgtccttata ttcacggcat
    48481 agggagcaaa ggcttgttgc ccctctatct gacgcaaaat ttctgacgca gttttcatca
    48541 atgcatccct tttatcatcg ggaagttcgc taatgaggag cgaaataagc agatcggaca
    48601 ccactcccac tgagtctctt gggtcagtca acttatcaac aggagccaca gcaagattag
    48661 ttgtgtaaat gctaaccagg tattcagtga tagcgtcctt aacgctttta tcaatcatgt
    48721 tagttacctt tttctggttg tgtggaaaca ccagaatacc acgcgccggg cgtggttaaa
    48781 catcccggca ctgattttca tttaacttaa acggggtttt ccgatatgac acagagacgc
    48841 agcttccgca ctggcacaga acgccacgca gcccgtttct caaccagcgc atcacgcagc
    48901 aactcgcact acagcctgca ggaaacgcac gccacacctg acgggtatct ggttaaacaa
    48961 ac
//
LOCUS       DAZGTJ010000029        45702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000029 DAZGTJ010000000
VERSION     DAZGTJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.029"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(29..2077)
                     /gene="prc"
                     /locus_tag="ACHK8E_002069"
     CDS             complement(29..2077)
                     /gene="prc"
                     /locus_tag="ACHK8E_002069"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP0344341.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(2097..2795)
                     /gene="proQ"
                     /locus_tag="ACHK8E_002070"
     CDS             complement(2097..2795)
                     /gene="proQ"
                     /locus_tag="ACHK8E_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP0344342.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(2892..3389)
                     /gene="msrC"
                     /locus_tag="ACHK8E_002071"
     CDS             complement(2892..3389)
                     /gene="msrC"
                     /locus_tag="ACHK8E_002071"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP0344343.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            3519..4802
                     /gene="yebS"
                     /locus_tag="ACHK8E_002072"
     CDS             3519..4802
                     /gene="yebS"
                     /locus_tag="ACHK8E_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP0344344.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4771..7404
                     /gene="yebT"
                     /locus_tag="ACHK8E_002073"
     CDS             4771..7404
                     /gene="yebT"
                     /locus_tag="ACHK8E_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP0344345.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            7484..8923
                     /gene="rsmF"
                     /locus_tag="ACHK8E_002074"
     CDS             7484..8923
                     /gene="rsmF"
                     /locus_tag="ACHK8E_002074"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP0344346.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            9041..9277
                     /gene="yebV"
                     /locus_tag="ACHK8E_002075"
     CDS             9041..9277
                     /gene="yebV"
                     /locus_tag="ACHK8E_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP0344347.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            9382..9573
                     /gene="yebW"
                     /locus_tag="ACHK8E_002076"
     CDS             9382..9573
                     /gene="yebW"
                     /locus_tag="ACHK8E_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP0344348.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(9574..10230)
                     /gene="pphA"
                     /locus_tag="ACHK8E_002077"
     CDS             complement(9574..10230)
                     /gene="pphA"
                     /locus_tag="ACHK8E_002077"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0344349.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(10625..10966)
                     /gene="yebY"
                     /locus_tag="ACHK8E_002078"
     CDS             complement(10625..10966)
                     /gene="yebY"
                     /locus_tag="ACHK8E_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP0344350.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(10979..11851)
                     /gene="copD"
                     /locus_tag="ACHK8E_002079"
     CDS             complement(10979..11851)
                     /gene="copD"
                     /locus_tag="ACHK8E_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP0344351.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(11855..12229)
                     /gene="yobA"
                     /locus_tag="ACHK8E_002080"
     CDS             complement(11855..12229)
                     /gene="yobA"
                     /locus_tag="ACHK8E_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP0344352.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            12368..12598
                     /gene="holE"
                     /locus_tag="ACHK8E_002081"
     CDS             12368..12598
                     /gene="holE"
                     /locus_tag="ACHK8E_002081"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0344353.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            12700..13356
                     /gene="yobB"
                     /locus_tag="ACHK8E_002082"
     CDS             12700..13356
                     /gene="yobB"
                     /locus_tag="ACHK8E_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP0344354.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            13380..14042
                     /gene="exoX"
                     /locus_tag="ACHK8E_002083"
     CDS             13380..14042
                     /gene="exoX"
                     /locus_tag="ACHK8E_002083"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP0344355.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(14039..16099)
                     /gene="ptrB"
                     /locus_tag="ACHK8E_002084"
     CDS             complement(14039..16099)
                     /gene="ptrB"
                     /locus_tag="ACHK8E_002084"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP0344356.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(16308..16967)
                     /gene="yebE"
                     /locus_tag="ACHK8E_002085"
     CDS             complement(16308..16967)
                     /gene="yebE"
                     /locus_tag="ACHK8E_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0344357.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(17294..17650)
                     /gene="yebF"
                     /locus_tag="ACHK8E_002086"
     CDS             complement(17294..17650)
                     /gene="yebF"
                     /locus_tag="ACHK8E_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP0344358.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(17717..18007)
                     /gene="yebG"
                     /locus_tag="ACHK8E_002087"
     CDS             complement(17717..18007)
                     /gene="yebG"
                     /locus_tag="ACHK8E_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP0344359.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            18141..19319
                     /gene="purT"
                     /locus_tag="ACHK8E_002088"
     CDS             18141..19319
                     /gene="purT"
                     /locus_tag="ACHK8E_002088"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP0344360.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(19375..20016)
                     /gene="kdgA"
                     /locus_tag="ACHK8E_002089"
                     /gene_synonym="eda"
     CDS             complement(19375..20016)
                     /gene="kdgA"
                     /locus_tag="ACHK8E_002089"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0344361.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(20053..21864)
                     /gene="edd"
                     /locus_tag="ACHK8E_002090"
     CDS             complement(20053..21864)
                     /gene="edd"
                     /locus_tag="ACHK8E_002090"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP0344362.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(22099..23574)
                     /gene="zwf"
                     /locus_tag="ACHK8E_002091"
     CDS             complement(22099..23574)
                     /gene="zwf"
                     /locus_tag="ACHK8E_002091"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP0344363.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23912..24781
                     /gene="hexR"
                     /locus_tag="ACHK8E_002092"
                     /gene_synonym="yebK"
     CDS             23912..24781
                     /gene="hexR"
                     /locus_tag="ACHK8E_002092"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP0344364.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24909..26351
                     /gene="pyk"
                     /locus_tag="ACHK8E_002093"
     CDS             24909..26351
                     /gene="pyk"
                     /locus_tag="ACHK8E_002093"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP0344365.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(26483..27454)
                     /gene="lpxM"
                     /locus_tag="ACHK8E_002094"
                     /gene_synonym="msbB"
     CDS             complement(26483..27454)
                     /gene="lpxM"
                     /locus_tag="ACHK8E_002094"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0344366.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(27574..28896)
                     /gene="mepM"
                     /locus_tag="ACHK8E_002095"
     CDS             complement(27574..28896)
                     /gene="mepM"
                     /locus_tag="ACHK8E_002095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP0344367.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(28912..29844)
                     /gene="znuA"
                     /locus_tag="ACHK8E_002096"
     CDS             complement(28912..29844)
                     /gene="znuA"
                     /locus_tag="ACHK8E_002096"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP0344368.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            29923..30678
                     /gene="znuC"
                     /locus_tag="ACHK8E_002097"
     CDS             29923..30678
                     /gene="znuC"
                     /locus_tag="ACHK8E_002097"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP0344369.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            30675..31460
                     /gene="znuB"
                     /locus_tag="ACHK8E_002098"
     CDS             30675..31460
                     /gene="znuB"
                     /locus_tag="ACHK8E_002098"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP0344370.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(31610..32620)
                     /gene="ruvB"
                     /locus_tag="ACHK8E_002099"
     CDS             complement(31610..32620)
                     /gene="ruvB"
                     /locus_tag="ACHK8E_002099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP0344371.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(32629..33240)
                     /gene="ruvA"
                     /locus_tag="ACHK8E_002100"
     CDS             complement(32629..33240)
                     /gene="ruvA"
                     /locus_tag="ACHK8E_002100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP0344372.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(33379..33444)
                     /gene="yobI"
                     /locus_tag="ACHK8E_002101"
     CDS             complement(33379..33444)
                     /gene="yobI"
                     /locus_tag="ACHK8E_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP0344373.1"
                     /translation="MCIFITHFFAEYVIFKYLLPI"
     gene            33515..34117
                     /gene="yebB"
                     /locus_tag="ACHK8E_002102"
     CDS             33515..34117
                     /gene="yebB"
                     /locus_tag="ACHK8E_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP0344374.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(34119..34640)
                     /gene="ruvC"
                     /locus_tag="ACHK8E_002103"
     CDS             complement(34119..34640)
                     /gene="ruvC"
                     /locus_tag="ACHK8E_002103"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP0344375.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(34675..35415)
                     /gene="yebC"
                     /locus_tag="ACHK8E_002104"
     CDS             complement(34675..35415)
                     /gene="yebC"
                     /locus_tag="ACHK8E_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0344376.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(35444..35896)
                     /gene="nudB"
                     /locus_tag="ACHK8E_002105"
     CDS             complement(35444..35896)
                     /gene="nudB"
                     /locus_tag="ACHK8E_002105"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP0344377.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(36014..37786)
                     /gene="aspS"
                     /locus_tag="ACHK8E_002106"
     CDS             complement(36014..37786)
                     /gene="aspS"
                     /locus_tag="ACHK8E_002106"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP0344378.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            38096..38662
                     /gene="yecD"
                     /locus_tag="ACHK8E_002107"
     CDS             38096..38662
                     /gene="yecD"
                     /locus_tag="ACHK8E_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0344379.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            38659..39477
                     /gene="yecE"
                     /locus_tag="ACHK8E_002108"
     CDS             38659..39477
                     /gene="yecE"
                     /locus_tag="ACHK8E_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP0344380.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            39530..39925
                     /gene="yecN"
                     /locus_tag="ACHK8E_002109"
     CDS             39530..39925
                     /gene="yecN"
                     /locus_tag="ACHK8E_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP0344381.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            39966..40709
                     /gene="cmoA"
                     /locus_tag="ACHK8E_002110"
     CDS             39966..40709
                     /gene="cmoA"
                     /locus_tag="ACHK8E_002110"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP0344382.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            40706..41677
                     /gene="cmoB"
                     /locus_tag="ACHK8E_002111"
     CDS             40706..41677
                     /gene="cmoB"
                     /locus_tag="ACHK8E_002111"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP0344383.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(41842..44271)
                     /gene="torZ"
                     /locus_tag="ACHK8E_002112"
     CDS             complement(41842..44271)
                     /gene="torZ"
                     /locus_tag="ACHK8E_002112"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP0344384.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(44296..45396)
                     /gene="torY"
                     /locus_tag="ACHK8E_002113"
     CDS             complement(44296..45396)
                     /gene="torY"
                     /locus_tag="ACHK8E_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP0344385.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        10845 a        11839 c        11302 g        11716 t
ORIGIN      
        1 aggcacaatt tcttgtgcct gattgatatt acttgacggg agcgggttgt tccgcgggtc
       61 tggctttttc aagcttcgcc agatcgagtg cgatattcac cgtctcatcc agataaggat
      121 ccggctcctg gtaatctttc ggtagatcat ccagtttctt caactccggt ttaccttcgc
      181 gtttaaagcg ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta ttctctttct
      241 cacgcacagc gtaattcaga gaaacgatat tgcgcttgtc cttcatggcg ttgaagcgcg
      301 cgatatcctt catgatgttc tggaactcag gatctttcgc gatacgcgca ttatgttcct
      361 tcagcagctc cggtccaaag gccgttaaat cgcctgattt cacataggtc gcggcatcga
      421 tgctatccca cggcagcgcg ttatcttcga atttctcacc cgtttccgtt tcttcattac
      481 ccgtcggcat gatgatgtct ggcgttacac ctttacgttg cgtactgccg ccgttaacgc
      541 gatagaattt ctggatcgtg tactgtacag atcccagcgc tggccattca ggacgtaaca
      601 tctgatcgta gatacggttc aatgaacggt attgctgaac ggtgccttta ccaaacgtcg
      661 gttcacccac aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag atttctgaag
      721 ccgaagcact gaaacggtca accagtacca ccagcgggcc tttatagaag acctgtccgt
      781 cggtatcgct gtcttcacga actttaccgt tgttatcacg gacctgaaca atcgggccgg
      841 atggaataaa cagaccggag agcgataccg cttcggtcag cgccccacca ccattgctac
      901 gcaggtcgat gatgacgctg ctgacattct gtttttccag tttctgcagt tgcactttga
      961 catcgtctgt caaacccaca tagaagcccg gaatatccag cacgccgact ttctctttac
     1021 cgacggtctt caccgacatt ttaaccgcgc ggtcttcgag gcgaatacgt tcacgggtca
     1081 acgttacagt acgggtcttg gtccctttac cagcaggtaa aatttccaga cgaactttac
     1141 tgcccttcgg ccctttaatt aaggcaacca catcatcaag acgccagcca atcacgtcaa
     1201 ccatcggctt gcctgtttga ccaacaccga caattttgtc accaacgctg atagctttac
     1261 tcttcgctgc cgggcctcct gccaccatcg aattgataac ggtgtagtca tcatccattt
     1321 gcagcactgc gccaatgcct tccagcgaca aactcatttc agtgttgaac tgttcggtat
     1381 tacgcgggga aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc gtcattgcca
     1441 gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat ttgtagcggc
     1501 gagtcagggt ttcacgaatt tctttatccg tttttcctgc cagcttcagg cttaactcgt
     1561 cgaatttgac tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc ggccagggcg
     1621 ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc ttttccagta
     1681 ccgacaaagc gtactggtaa cgttcaaaac gacgcttttg cgccagattg tagagatcgt
     1741 agaaaacgtc gagtttgcct gaacgcagtt catcgcctaa ctcggttttc tttttcgcga
     1801 actgttcaac atcgcttgcc aacagcacgt tgtggctgta atcgagcaga ttcaggtagc
     1861 ggtcaaagat tttggccgaa aatgtctgat cgaggtcgaa ctggcgataa tgagaacggg
     1921 tgaagcgcga cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc tttaataccg
     1981 gaatttgatc agcacgcgtg atatcttcta cagcgaaggt ctggcctgct attgcaagca
     2041 ggccagctaa cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc tccgtttcag
     2101 aacaccaggt gttctgcgcg cacaatcaaa gacatacccg aattcagctg gacgcggacg
     2161 ccgtctttgg tgatttctaa tacggtggca tccatcgcgt tttgacccgc tttcaccttc
     2221 agggcttgtc cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg ttcttcgcga
     2281 ggtgctttta ctgtttttgg cgctttctct accggctttt gcgcacgagg tttacgttca
     2341 gcgccttctt tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg cggtgcgtct
     2401 tctttctcac cagcagctgc ggcagcttcg cgttttttcg cttgctgttc agcacgctgt
     2461 gcctgaacac gcgctttcgc ttcttcaagc tgcttgcgag catgctctac atgttgctcg
     2521 tccagctcac cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg tttaacaccg
     2581 taaagataac gccagctcga agtgtagaga cgtaaagcgg atcgcaattg cgttttgctc
     2641 aggttcattt ccccggcaac acgatcgacc aaatcctgaa aaataccgat tttcagcgga
     2701 cgcgcttcac cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa cgcgattact
     2761 tctttactgc tattcaactt aggttgattt tccatgaaat ttcctgatta caacggacgt
     2821 agccaacaag cgcaggcatg aacaggcgtc attataatga cgctatcagt aaatgctacg
     2881 ttatccgttg attatcctgc gacgctcgca aagaattttt tgtaatccgt cgttgcaagc
     2941 actttttcaa gctgtgccac aagctgacgt aagccttgct cgtcctcgtc tgtaaagcga
     3001 ccgaagacgg tactatcgat gtcgagaaca ccaataatct gatttttcac caccagcggc
     3061 agaacaattt cagaattact cgccgcatca caggcaatat gcccgtcaaa cacatgaaca
     3121 tcctcgatac gctgcacttg attgcgggca accgcagtgc cgcacacgcc gcgcccgacg
     3181 ggtatccgga cacaggcaat tttgccctga aatggtccga gtaccagtgt atcgtcctca
     3241 agcaaataaa aacctgccca gtttacgtca gtgagacgct catataacaa cgcactggtg
     3301 ttcgcaagcg ttgccagaaa actggtttct cctgccatca gcgcgttaaa gtcgcgattt
     3361 aaatccgcgt aaaattctat tttgttcatt atgtaatcac ttggttgtct taccaggaac
     3421 tgccagccta ttaaaataag cattaaatgc gttaatgctc aagatcattc caatcatggg
     3481 ttaagattaa tgttaattct ttttacattt ggcacgtcat ggctcttaac acaccacaaa
     3541 ttacgccgac aaaaaagata acagtgaggg ctatcggtga ggaactgccg cgtggtgatt
     3601 accaacgttg cccgcaatgt gacatgctgt ttagcctgcc cgagataaat tctcatcaaa
     3661 gtgcctattg tccgcgctgt caggcaaaaa ttcgtgacgg gcgcgactgg tcgctaacgc
     3721 gcctggcggc aatggccttc accatgctgt tgctgatgcc gtttgcctgg ggcgaaccgc
     3781 tgttgcatat ctggctgtta ggcattcgta ttgacgccaa cgtcatgcaa ggcatctggc
     3841 aaatgaccaa acagggcgat gcgataacgg gatcaatggt ctttttttgc gttattggtg
     3901 cccccctcat tctggtgacc tccatagctt atttatggtt tggtaaccga ctgggaatga
     3961 atctacgccc ggtactgctg atgcttgagc gacttaaaga gtgggtaatg ctggatatct
     4021 acctggtcgg cattggcgtt gcttctataa aggtacaaga ttatgcccat atccaggcgg
     4081 gtgtcggctt gttctctttt gtggcgttgg tgattttaac gacggtgacg ttgtcacatc
     4141 tcaatgtcga agaactgtgg gagcgatttt atccacagcg ccccactacg cgtagagacg
     4201 agaaactccg tgtctgtctt gggtgccatt ttaccggcta tcctgatcag cgtggtcgct
     4261 gcccgcgttg ccatatcccg ctacgcctgc gtcgccgtca tagtctgcaa aaatgctggg
     4321 cggcgctgtt agcgtcaatc gttttgttgt tacctgccaa cctgttgcct atttctatca
     4381 tttatctgaa tggtggccgg caggaagata cgattctttc cgggattatg tcgctggcaa
     4441 gtagcaacat tgccgttgcg ggaatcgtgt ttatcgccag tattctggta ccgtttacta
     4501 aagtgatcgt catgttcact ttactgttga gtattcattt taaatgccag caaggtttac
     4561 gcacacgcat tctgttactg cggatggtga cctggattgg tcgctggtcg atgctcgacc
     4621 tgtttgtcat atctttaacc atgtcgctga ttaatcgcga tcagatcctc gcttttacta
     4681 tgggaccggc tgcgttttat ttcggcgcag cggtaatttt gactattctt gctgtggaat
     4741 ggctggacag ccgcttactt tgggatgcac atgagtcagg aaacgcccgc ttcgacgact
     4801 gaagcgcaga ttaaaaataa acgccgtatc tcacctttct ggctgctgcc gttcatcgcg
     4861 ctaatgattg ccggttggct gatttgggat agttatcagg accggggtaa taccgtcacc
     4921 atcgacttta tgtcggcgga tggtattgtc ccgggccgca cccctgttcg ttatcagggc
     4981 gttgaagtcg gaactgtgca ggatatcagc ctgagcgacg atcttcgtaa gattgaagtc
     5041 aaggtcagca tcaagtctga tatgaaagat gcgctgcgcg aagagacaca gttctggctg
     5101 gtgacgccaa aggcctcact ggcaggtgtc tccgggctgg atgccctcgt cggtggtaac
     5161 tatatcggca tgatgccagg taaaggtaaa gagcaggatc actttgtcgc actcgatacc
     5221 caaccgaagt atcggctgga taatggcgat ctgatgatcc acctgcaagc ccccgatctc
     5281 ggttcactga acagcggttc attggtctat ttccgtaaga tcccggtggg taaagtctac
     5341 gactatgcca tcaatcccaa taagcaaggc gtggtgattg atgtcctgat cgagcggcgt
     5401 tttaccgacc tggtgaaaaa aggtagccgt ttctggaacg tttccggcgt tgatgccaac
     5461 gtcagtatca gtggcgcgaa ggtgaaactg gaaagtctgg cggcactggt taacggtgcg
     5521 attgccttcg attcacctga agagtcgaaa cctgccgagg cggaagatac ctttggtctg
     5581 tatgaagatc tcgcccacag ccagcgtggc gtaataataa aactggaact gccgagtggg
     5641 gccggattaa ccgccgactc gacgccgtta atgtatcagg ggctggaagt cggacagctg
     5701 actaaactgg atttaaatcc tggtggtaaa gtcaccggag aaatgaccgt tgatcccagc
     5761 gtcgttacgc tgttacggga aaatacccgc atcgaattac gtaatccgaa attatccctt
     5821 agcgatgcca atctcagcgc cctgctgacc ggaaaaacct ttgagttggt gcccggcgat
     5881 ggtgagccac gcaaagagtt cgttgttgtg ccaggcgaaa aagccctgct acaagagccg
     5941 gatgttctga cactgaccct gacagcacca gaaagttacg gtattgatgc tggtcagccg
     6001 ctcattctcc acggtgtgca agtaggccag gttattgatc gtaaactcac cagcaaaggc
     6061 gttaccttta ccgtcgccat cgagcctcag catcgcgaac tggtaaaagg cgatagcaaa
     6121 tttgtcgtca acagccgtgt cgacgtgaag gtggggctgg atggcgttga gtttctcggt
     6181 gccagcgcct cagaatggat taacggcggg atacgtattc tgccgggcga taaaggcgag
     6241 atgaaagcca gctatccact gtatgccaat ctggaaaaag cgctggagaa cagccttagc
     6301 gatttaccca ccacaaccgt aagtttgagt gcagagacgc tgccggatgt gcaggcagga
     6361 tcggtagtgc tgtaccgtaa atttgaagtt ggtgaagtta ttactgtgcg tccgcgagct
     6421 aacgcgtttg atatcgatct gcatattaag ccggagtatc gcaaccttct gaccagcaac
     6481 agcgtgttct gggcagaagg tggggcgaaa gttcagctaa atggtagtgg tctgaccgta
     6541 caggcatccc cgctctccag agcattaaag ggggctatta gcttcgataa cctcagcggt
     6601 gccagcgcca gtcagcgtaa aggcgacaag cgtattctgt atgcttccga aacagcggcc
     6661 cgtgcggttg gcgggcaaat tacgcttcac gctttcgatg ccggaaaact ggcggtcggg
     6721 atgccaattc gctatctcgg tattgatatc gggcaaatcc agacgctgga tctgattacc
     6781 gcgcgcaatg aagtacaggc aaaggcggta ctttacccgg aatatgtcca gacctttgca
     6841 cgcggtggta cacgcttctc agtggtcaca ccgcaaattt cggcagctgg cgttgagcat
     6901 cttgatacta tcctccagcc gtatatcaac gtcgaaccag gccggggcaa tcctcggcgc
     6961 gactttgaat tacaagaggc taccataact gattcgcgtt acctggatgg cttaagcatt
     7021 attgttgaag cgccggaagc cggttcgtta ggcatcggta cgcctgtgct gttccgtggt
     7081 ctggaagtcg gtacggttac cggaatgacg ctggggacat tgtcagatcg cgtgatgatt
     7141 gcgatgcgca tcagtaaacg ctatcaacac ctggtgcgta acaattccgt cttctggttg
     7201 gcatcgggtt acagtctgga ctttggtctg acgggcggcg tagtgaaaac cggcaccttt
     7261 aaccagttta tccgtggcgg catcgccttc gccacgcctc cgggtacgcc actggcaccg
     7321 aaagcccagg aaggcaagca cttcctgttg caggaaagtg aaccgaaaga gtggcgtgaa
     7381 tggggaactg cgcttcccaa ataatgccca ctgctccggc gtgcctgcgc cggagcgttt
     7441 atgctaaact gcgcgcctgt ttttttgcca gtggtatatg ctcgtggccc aacacaccgt
     7501 ttatttcccg gacgcctttc tgacacaaat gcgcgaagcg atgccttcga cgctctcttt
     7561 tgatgatttt cttgccgcct gtcagcgccc gttgcgccgc agcattcgcg ttaatacgct
     7621 gaaaacctcc gttgctgatt tcctgcaatt aactgctcct tatggctgga cgcttacgcc
     7681 aattccgtgg tgtgaagaag gtttctggat tgaacgcgac agtgaagatg cattgccgtt
     7741 gggtagtacc gccgagcatt taagcggcct gttttatatt caggaagcca gttcaatgtt
     7801 gcctgttgcc gccttgtttg ctgacggtaa tgcaccacag cgggtgatgg atgtcgctgc
     7861 cgcgcccggc tccaaaacga cgcaaattgc cgcgcggatg aataacgaag gggcaatcct
     7921 tgccaatgag ttttccgcca gtcgggtaaa agtgttacat gccaatatca gccgctgtgg
     7981 catcagtaat gttgcgctca cgcattttga tggccgcgta tttggtgcgg cagtgccaga
     8041 aatgttcgat gccattttgc tggacgctcc ctgctccggc gaaggcgtgg tgcgtaaaga
     8101 tcccgatgcg ctaaaaaact ggtcaccaga aagcaatcag gaaatcgcag cgacccaacg
     8161 ggaactgatc gacagcgcct ttcatgcatt acgtcctggc ggtacgctgg tttactcgac
     8221 ctgtacctta aaccgggaag aaaacgaagc cgtttgcctg tggctgaaag agacttatcc
     8281 cgacgcagta gagtttttac cgcttggcga tctcttccct ggtgcaaaca aggcgctgac
     8341 cgaggaaggc tttttgcatg ttttcccaca aatttacgac tgcgaaggct tcttcgttgc
     8401 tcgtctgcgt aaaactcagg cgattcccgc cttacccacc ccaaaataca aagtgggcaa
     8461 tttcccgttc agcccggtga aagatcgcga agccggacaa attcgtcagg cggctgcaag
     8521 tgttggctta aactgggatg aaaacctgcg actctggcag cgtgacaaag aactgtggtt
     8581 gttcccggtg ggcattgaag ccctgatcgg taaagtccga ttttctcgct tggggattaa
     8641 acttgccgaa acgcacaaca aaggttatcg ctggcagcat gaagccgtta ttgcccttgc
     8701 ctcccccgac aatgtgaacg cttttgaact gacaccgcag gaagcggaag agtggtatcg
     8761 cgggcgcgat gtttacccgc aagccgcgcc agtagcggat gatgtattgg ttactttcca
     8821 gcatcagccg attggtttag ccaaacggat tggttcgcga ctgaaaaaca gctacccgcg
     8881 tgaactggtg cgcgatggga aactttttac cggtaacgcc tgacagcgca caaaaaaagc
     8941 gcactttttg actggcacat tcggctgcct caactaggct gaaaaatggt gcgatcggac
     9001 tggtcgtacc acaaccggca gctaaatgga gagcacgaag atgaaaacca gtgtgcgcat
     9061 aggcgctttt gaaatcgacg acggcgaatt acacggcgaa tcgccgggtg atcgaacgtt
     9121 aaccattcct tgtaaatcag accccgattt atgtatgcaa ctggatgcct gggatgctga
     9181 aaccagtatt cctgccctgc taaatggcga acactctgtc ctttaccgta cccgttacga
     9241 tcaacaatct gatgcctgga ttatgcgtct tgcctgatcc aaaaagaacc cgtcggcatg
     9301 gcgggttatt tgtcctggtt atttccccgt tgtaaaatct ctcctaaact taacggtacg
     9361 gcaccacact tcggggatga aatgttcgcg ctggtacttt ttgtttgcta cctggatggc
     9421 ggttgtgaag atattgttgt ggatgtctac aacacggaac agcagtgtct ttattctatg
     9481 agcgatcaac ggatccgcca tggcggttgt tttccaatcg aggattttat tgatggtttc
     9541 tggcgaccag cacaggagta cggtgatttt taattattgc aattgcacaa gagtcagttc
     9601 gcccccaaag acagcaccgg tatcaatata atgcaggttg ccaatatcca cgcgatgtcg
     9661 caacggtgta tgaccaaacc agaaatgatc agcaccttta attccctgcc cttttgggcg
     9721 ttcacctaat cgcgagcggc tccacaagac ctgatgcaaa tcaacgtcct tttgccattc
     9781 ataaacatca tccggataat cggcatgggc aataacatgt ttgccggtgc gactgtgtac
     9841 ttcaagaata aagggcaaat gctgacattt ttccagcgcc gttttcgctt gtttctgttg
     9901 attatctgcc agcgcaataa accagtcgcc gccattcatg aaccacaaag acatctgctg
     9961 ggatgccagc gcatccatcg ccatctgttc atgattgcct cttaccgcac gaacccaatg
    10021 ttgttccagt aactgcaggc aacgtaaact ttgcggccca cgatcgataa cgtctcccac
    10081 tgagataagt aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg
    10141 ctcaagacaa ccgtgtatat cgcctgaaag ccagatatgt cgccattgat gacccgcaat
    10201 tctctgataa acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca
    10261 taatcttaac aagaatgtta aaaaacgctg gacgcagaca gtagagtgtg tgttatggtt
    10321 gactataaag tcagcgaagg gaatgcttct ggcttttaac agataaaaag agaccgaaca
    10381 cgattcctgt attcggtcca gggaaatggc tcttgggaga gagccgtgcg ctaaaagttg
    10441 gcattaatgc aggcttagtt gccttgctct ttaagaatag atgacgacgc caggttttcc
    10501 agtttgcgtg caaaatggtc aataaaaagc gtggtggtca tcagcttaaa tgttaaaaac
    10561 cgcccgttct ggtgaaagaa ctgaggcggt tttttattgg aaatcaaaag gctattttag
    10621 gtaattaaca gagtttttca gctcgctcta taaacggtgc cagactcatt ttttcgccgg
    10681 gattgttagg atcatcaatc tgaatcaccg aaatgggttg ggctttagtc ttcccactgg
    10741 caacttcctt ttgtgcgata tcgtttaaag gatactgcac gagggtactt gggttgatga
    10801 catacaaagc attacccggt cggcaagtca gcatcacctc ttcgcgatta aacgcccatt
    10861 tgtctttacc cacttcaaaa cggctgacgg taatcacctg cggtgccgcc agcgccgctg
    10921 cagaactggt gagtaacaga aacgccagaa tacttttttt catcattttc gtaatccatc
    10981 aaaaaggttc gagagtcgca agcaggctga tgatcaccag cactaccgca cctatcgccc
    11041 attcgagttt agtcatccac acaaaccagg gagccgcacg atcttcatcc tgcctcatgc
    11101 gcggtacaag aacataacga ttagccagcg cgatgaccac cataatcatc accaggatgg
    11161 ctttgagcat taagagttgg ccccagtaag tcgtaagcgt ggggggaaat ccggtaatca
    11221 acaatgcatt aagcacgccg cttgccagta cgccgatcac cgcaaaatgc ccgcaccagg
    11281 aaaaacgcat cagcgcctga atagcctggt gtcgccaacg gcctttgatg agttgcatac
    11341 accagagcac cggcagtaat cccccaaacc acgccgccgc acagatcagg tgaattgcgt
    11401 gattagtctg atggattttc gccgttaccc cttcattcag cgtcgcatgc ccgactcccg
    11461 ccagcagaat aaattgcgcg gtagtgagca taaatagcaa acgtggcata ttctgcggtt
    11521 gcataagggc aacgatcaac gttacgaggg cgagaacaat ttgccataac cagatgccac
    11581 caaactgcgt ttgcaaaaca gccagccaaa tgtttggcga aaatacatct gaccatcccg
    11641 ttcccatcaa cccgccctga acggcgagca ttgcagtggc actgatcaaa ctccagacgg
    11701 cagcgtgctg ttgtaaacgc agaaaacgct tcgtcagcaa gcgacgaatc gttaaggggg
    11761 ccagccaggc accgtacatt gcgaagccaa aaaccagcat caacgaggtg aaatggataa
    11821 atcgcagcgc gatccaggta aacgccagca tgatttattt cacgctaaag gtgtagtgtc
    11881 ctttcgtttt atgcccgtcc accgaaacaa catgccagtc aacggtgtag gttccgggtt
    11941 tcagcgaatc ggccagtgga acaatcagtt gcttttggtc ctgctcattc cgcttcgccg
    12001 gtaatgtttt aatattttcg ttttttggcc ccgtgatttt tgcaccactg aatcctgttt
    12061 caacgccttc cgagaagttt aaagtgattg cctgcggggc agctgtcact tgcgcatttg
    12121 ccgcaggata ctgatgcgtt aaatgcgcat gtgcccagac ggaaggtgtc accaacgaag
    12181 tggtcaggat tgccagcgcg tagcgaaggg agcgtgcagt tgaagccata ttatctattc
    12241 ctttttgtaa taactttttt acagagcata accttgtcta atgtctgagt cgaggatcat
    12301 caattccggc ttgccatcca ggctcactct tagtaacttt tgcccgcgaa tgatgaggag
    12361 attaagaatg ctgaagaatc tggctaaact ggatcaaaca gaaatggata aagtgaatgt
    12421 cgatttggcg gcggccgggg tggcatttaa agaacgctac aatatgccgg tgatcgctga
    12481 agcggttgaa cgtgaacagc ctgaacattt gcgcagctgg tttcgcgagc ggcttatcgc
    12541 ccaccgtttg gcttcggtca atctgtcacg tttaccttac gagcccaaac ttaaataaaa
    12601 cttatacaga gttacacttt cttacataac gcctgctaaa ttatgagtat tttctaaacc
    12661 gcacccataa tttgcagtca ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca
    12721 gcagcgcaat atgagcctcg taaaacctca ctgaccgaac aggtagctca tcatctggag
    12781 tttgtgagtg ccgccgccag gcaacaatgt cagctattag tttttccatc gctttcttta
    12841 ctggggtgtg attattcgcg acgtgccctt cctgccccac ccgatctctc gctgttagac
    12901 ccgctttgtt atgctgcaac gacctggcga atgaccatca ttgccggcct tcccgttgaa
    12961 tataacgatc gctttattcg tggaattgcg gtgttcgcac cgtggcgaaa aacaccaggg
    13021 atctaccatc agagtcatgg tgcatgtctg ggccgtcgtt ccagaaccat cacggtggtc
    13081 gatgaacaac cggaaggtat ggatatggac ccgacctgtt cactgtttac gacagggcaa
    13141 tgtctggggg aacccgacct actggcatct acccgccgct tacaattttt ttcacatcag
    13201 tactcaattg ccgtgctgat ggcaaatgcc cgtggtaaca gtgcactatg ggatgaacat
    13261 ggtcgtctca tcgttcgcgc cgatcgtggt tcattattgt tagtcggtca gcgttcatca
    13321 cagggttggc aaggcgatat cattccatta cgctaggctt tttcggcctg gagcatgcca
    13381 tgttgcgcat tatcgataca gaaacctgcg gtttgcaggg agggatcgtt gagattgcct
    13441 ctgttgatgt cattgacgga aaaatcgtca accccatgag ccacctggtg cgccccgatc
    13501 gtcctattag tccacaagcg atggcgattc atcgcatcac cgaagccatg gtcgccgata
    13561 aaccgtggat tgaagatgtg atcccacact attacggtag tgaatggtat gtcgcgcaca
    13621 acgccagctt tgaccgccgc gtactgcctg agatgcccgg tgagtggatt tgcactatga
    13681 aactggcccg tcgtttgtgg ccagggatca agtacagcaa tatggcgtta tataaaacac
    13741 ggaagctcaa tgtacagacg ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt
    13801 atatcaccgc tgcgttgctt atcgatatta tgaacacctc cggctggacg gcagaacaga
    13861 tggccgatat caccggacgt ccgtcgttga tgacgacctt cacctttggc aaataccgtg
    13921 gcaaagcggt ttccgacgtt gccgaacgcg atccgggcta tctgcgctgg ttgtttaata
    13981 acctggacag catgagcccg gagctgcgtt taacactgaa acattatctg gaaaatactt
    14041 aatcctcagg cgtagcgggt aatgttccct gcgccagcgc gaccagaaaa gcatattcca
    14101 tcgctacgcc ttcgtacgat ttaaagcgac cagatttacc gccatgacct gagtccatgt
    14161 cggtacagag caataaaaga tggtcatcgg ttttcagctc gcgcaattta gcgacccatt
    14221 ttgccggttc ccaatattgc acctgagaat cgtgcaaacc ggtcgttacc agtaaatgcg
    14281 gataagcctg tgcggtgacg ttgtcatatg ggctgtagct tttcatgtac tcgtaatatt
    14341 gcggatcctg cggattcccc cactcttcaa actcaccagt ggtaagagga attgattcat
    14401 caagcattgt tgttacaaca tcaacaaacg gtacctgggc gataacgccg tggaataatt
    14461 ccgggcgttg attaattgca acgcccatca acatgccccc cgcacttccg cccatcgcat
    14521 aacagagcga aggagagcca tagcccagtt ttaacaatgc atcgcaggca tcaagataat
    14581 cattaaacgt atttttcttc ttcagaaatt taccgtcttc gtaccattgt tgccccagct
    14641 caccaccgcc gcgaacatgg acaatggcgt agacaaagcc acgatctaac aaactcaagc
    14701 ggctaaaact gaaatcggca tcaatacttg cgccgtaaga accgtagcca tacaccagca
    14761 gcgggttgtg tcctttgcga aaatgtttgc gatgatagac cagcgaaacc ggaacttcga
    14821 cgccatcacg ggcgactatc cacaggtgtt cactgcggta attcgccgca tcaaaaccag
    14881 gaacttccgt ttgttttaat acacgacgct caccggtatc catatccagt tcaaacaaag
    14941 tatctggtgt ggtcatggaa gaataaccat aacgcaatcg cgcggtttca ggttctggat
    15001 tgtaggcaat ccaggtcaca taggccggat catcaaaggc aatgccaatg acttcccggg
    15061 tcttgcggtt gatttggcgc aaactggtta acccgcgctg acgctcttca accaccagcc
    15121 agtcggtaaa cagcgtaaac ccttccagca taatgttatc acgtggcgga attaactctt
    15181 cccactgttg ctcatcacgc atacgggtac ggtataagcc aaagttttta ccgtggcggt
    15241 tggaacgcag ataaaaccga tgctggtagt gatcaaggct gtattcgtga tctttgcggc
    15301 gaggcagaaa aacaaacggc tcggcatcgg ccatttccgc gtccagcagg cgaacttcac
    15361 tggtggtggc gctggccaga tgaatgacca cataatgctt cgaggtcgtt ttatgcaggc
    15421 tgacgtaata ggtatcgtct ttttcttcgt aaatcagttt atcttgcgat gctggcgtac
    15481 cgatggcgtg acgccagacc tgataaggca gcagcgtcac tggatgcttg cgaacatagt
    15541 agaaagtcca ggagtcattt gcccagacaa agctgggttc aacgttatcc agcagttccg
    15601 ggtaccagtt acctgtttcc agattacgaa aacgaatgcc atactgccgt cgggaaagaa
    15661 aatcttctgc cagtgccata atggtgttat ctggcgtaat cgccattccg cccatcgaat
    15721 aaaactcact atgagccgcg cgcttgttgg catcgagcaa tgtttcccac tcgtcccact
    15781 cttcactgaa cgccgattga cgctggtaga tagcatattc acagcctggt tcataaatat
    15841 gccgatagcg gtagccattt ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt
    15901 cgatgatttc ctttaagatg cgatcctgca aggcttgttg cgaggccatc acccgatgac
    15961 cgtaactatt ttcttgttgc agatagtcca ggactttcgg ctgagaacgc gtatcgtccc
    16021 gcagccagta gtaattatcg atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc
    16081 gggcggcttt tggtagcatg ttattgttct ttctggttga aacatcttat aagggtggca
    16141 aaactcaccg gggatgcaag cgaaacaggg tagtcattgc ttagatgatg acaggtaatg
    16201 gcgcggatat cgaatgttat gcagacagag aaaatcagcc tgctcaaatg gctgtgcggt
    16261 tctggataac ccgaaataat cagcttcagg ctatccagag agcggaatta ctccgccaaa
    16321 gtacgttttt gctgttcgag atcgcgttca atgccttcac gaacatcctg ggggattttc
    16381 agcgcatcac ccagtgcatt caggtaactg cgttccataa aatggtcaat atcaatagcc
    16441 gcgcagctca ggaaatagat ttccagcgcc tcttcttcat tgcggacccc ggtagccagg
    16501 cgttgtggat ccagcggttg ttcgattgcc tgctcaatga gtacacgccc cttctcttcc
    16561 acaccggctt ctcgcaattg ctggtcgatt gccgcacgtt ctttggcatc aatatggcca
    16621 tcacttttag cggcaaagac cagcgcaagg atcaaacgtt ccgtacgctc atccagcggc
    16681 gtactttgcg cgccaaactg cggttcatcc tgatgcgccg cgcgaatttt atctttgtat
    16741 ttattccaca gcaccgtacc cgctaccgct ccgccgccaa ccagtaacgc gtttgtgcca
    16801 tatttcgtaa gaagtttacg tgctgattta tttgcgacca gcagcccagc cagtccgcct
    16861 aatgcgccag gcactaaaag tttgcccaat ccctgatccg cagacgagga ggtagaagaa
    16921 cggctttgcc caagcaggga ttgcagttga tttaaccagt tagccatatt tgctcctcaa
    16981 taaccatttt tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt atattgagac
    17041 aaaagatgcg caaataagcg gggcggtagg ccgttattca aagaaattcg cgccaggtaa
    17101 ggtgttcact gtatataagg tgataagggt attttaggtg aaagtagttt gacgttttga
    17161 gtggaactgt ttgatgctga ttgcccggtt cgacgctgct gcgtcttctc aggactacga
    17221 ttgtgttgcg ttgtagggcg gacaaggcac tcgcgccgta tccgccaacg gtcaatcgaa
    17281 ttatcagaag ccattaacgc cgctgatatt ccgccattcc cgctttgcag tccacgctga
    17341 cctggtaatg aatatcggca cttttaccac gtacggttag cggtactgac catttatcat
    17401 ctttcccctg aatgtcctgc aaactgaccc atgccacggg atcgggctga ccgacaattt
    17461 tttgatcatc agcccagcgc gccacgcgat tttgttggta atcacgtttt acgctcgcgg
    17521 caattccggc agcatccaga ccttcacact ttgggaaagt gaccgacttg ctggtttcat
    17581 tattggcagc gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc
    17641 tttttttcat gtttttctcc atagcacaat gattcaggag aaagcatggt acaaattgtc
    17701 aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga
    17761 cgacctgtgg ggacggtaat ttgccggttt tcagaatggt gctcagcaca tctttttgtt
    17821 ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc catttgcact ggagaatttg
    17881 tcagccaggt gtcaagcaga tccgccgtat caagcatttt gtcataagca tcggcttcct
    17941 ttttgctggt aaacgacatt ttctcttcgc cctcacgaat gactacgtat ttaacttcaa
    18001 ccgccatttg cagcctctca taataactgt gattttatac agtatatttc ttctcgattg
    18061 ggaaatcaac atcaacaata aagacacgcg caaacgtttt cgtttatact gcgcgcggaa
    18121 taaatcaggg gatattcgtt atgacgttat taggcactgc gctgcgtccg gcagcaactc
    18181 gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc
    18241 tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc accagccatg catgtcgcgc
    18301 atcgctccca tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg
    18361 aaaaaccaca ttatatcgtg ccggagatcg aagctattgc caccgatatg ctgatccaac
    18421 ttgaagagga aggactgaat gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc
    18481 gcgagggtat ccgtcgcctg gcagcagaag agctgcagct gcccacttcc acttatcgtt
    18541 ttgccgataa cgaaaacctt ttccgcgagg cggttgctga aatcggctat ccctgcattg
    18601 ttaaaccggt gatgagttcc tccggcaagg ggcagacgtt tattcgttct gcagagcaac
    18661 ttgctcatgc atgggagtac gctcagcaag gcggtcgcgc cggagcgggc cgcgtaattg
    18721 ttgaaggcgt cgttaagttt gacttcgaaa ttaccctgct aaccgtcagc gcggtggatg
    18781 gcgtccattt ctgtgcaccg gtaggtcatc gccaggaaga tggcgactat cgtgaatcct
    18841 ggcaaccaca gcaaatgagc cagctggctt tggaacgtgc gcaagagatt gcccgtaaag
    18901 tggtgctggc actgggcggt tatgggctgt ttggtgtcga gctgtttgtc tgtggtgatg
    18961 aggtgatttt cagtgaggtc tcccctcgcc cacatgatac cgggatggtg acgttaattt
    19021 ctcaggatct ctcggagttt gccctgcatg tgcgagcctt tctcggactt ccggttggcg
    19081 ggatccgtca gtatggtcct gcagcttctg ccgttatcct gccacaactg accagtcaga
    19141 atgtcacgtt tgataatgtg cagaatgccg taggcgcagg catacagatt cgtttatttg
    19201 gtaagccgga aattgatggc agccgtcgtc tgggtgttgc gctggcgacc gctgagagtg
    19261 ttgatgaagc gattgagcgc gcaaagcacg ccgccggaca ggtaaaagta cagggttaaa
    19321 cccggcaaaa aaacgctaca aaaatgcccg atcctcgatc gggcattttg acttttacag
    19381 cttagcgcct tctacagctt cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc
    19441 cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt tcagcgccag
    19501 gtagtcacgg tagttagccg gagaaatacc acctgtcggg cagaaacgga cctgggagaa
    19561 cggacccgcg atcgcctgca gggctttcac accgccgtta gcttcagccg ggaagaattt
    19621 gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc tgatccccgg
    19681 aatcagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca gacccgggct
    19741 aattgcaaac tgtgcacccg cttcagtgac ttctgccagc tgctgtggat tcagcaccgt
    19801 accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag cgtcaactgc
    19861 gcactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca acgcttttgc
    19921 catcggcacc gcgtgttcca gtttttttac cacgataacc ggtacaaccg ggccggtggt
    19981 caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc gcctgattac
    20041 aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg acagtttttc
    20101 acgcaaggcg ctgaataatt cacgtcctgt tcccacgcgt gacgcgctca ggtcaggaat
    20161 gtgtggttcg cgagcagcca gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg
    20221 tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc
    20281 ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga gtcgtccatc
    20341 agtaactaac gcaattttga aacaccggtc caataataca ccaagtggcg gcatgagttt
    20401 atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa cgacacaatc
    20461 gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt caaaaacaac
    20521 cgcaggcgct tcaatcacct ggttctctac cggcacggca gaggttttca taaccgcacg
    20581 gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt gttcgaagga
    20641 agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca gttcaccatt
    20701 attcagccat ggttcaaggg tataacgaga cagaccaaag cctgccaccg tattgacatc
    20761 ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc cacctgccgc
    20821 ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca gcggtacaac
    20881 atcagaaagg tcagagaagt catcccagtt aatctgaata ccggctgcgc gcgccatcgc
    20941 caccaggtgc atggtgtggt tagtggaacc accggtcgcc agcagtgcaa cgataccgtt
    21001 caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac cattaccggt
    21061 catgcgcgta acctggcgcg cagctgcagc ggtcaaagca tcgcgcagcg gagaatccgg
    21121 atgaacaaaa gaagagcctg gcaactgcat ccccataaac tccaccacca tctggttggt
    21181 gttggcagta ccgtagaaag tacatgttcc cggcgcatga taagacgcgg cttctgactc
    21241 cagtagtgcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac gcactttttc
    21301 tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag gcaaatgccc
    21361 aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac cgaggaacag
    21421 agcaccatca aacatgttat gggacagccc caccgccgca gacatcgcaa tcacttcgcg
    21481 gcttaacagc gacaattcca ttccatcctg cccctgggtg acaccatcac acatcgccgg
    21541 aacaccgcct gcaacctgac caaccgcatt tgcttcatgc agagctttac gaatgatttc
    21601 tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg tgatgatggc
    21661 gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc aggcagcgaa
    21721 accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgaag ttttcgcttg
    21781 ttctatccgg gcgaggtaag cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt
    21841 tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag agcaggatga
    21901 ttcacaacac gtttcattca gaggattcat gactgaaacg cctgtaaccg gagctcatag
    21961 ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc
    22021 tgaaagtgta aaaattgttc tacaatctgt gcaagatcat gttaccggta aaataaccat
    22081 aaaggatgag cgcagatatt actcaaactc attccaggaa cgaccatcac gggtaatcat
    22141 cgccactgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg catcattgtc
    22201 catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca cttcatcgcg
    22261 acgtacgaac agtgcctgaa taccacgcat ggtttccagc agcagacgtt cataggcatc
    22321 tgccagatgc gtttgattaa aggtttctga atagctcaga tccagcttgg tgatttgcag
    22381 gttatgttta tggtcaaggc caggaacttt atttagtacc tggatatcca cgccttcatc
    22441 aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgact ctttaaacag
    22501 attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg gcagacgttt
    22561 accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt cgacgcggat
    22621 cgccacgaag gtttctgtat tgctgctctt gttcgcgccc tcttcttcca gatatcccgg
    22681 cacttttttg ccctgggcga agcccgcagt atattgcccg cgtacggttt tttcgcgcac
    22741 gttggagcgg tcaatgcggc gcagagactt cagtactttc actttttcat cgcggatgct
    22801 gtctgcactc aggtcagacg gcggagacat cgcaatcatg caaagaattt gcagcaggtg
    22861 gttctggatc atgtcgcgca tctgaccggc tttatcaaaa tagccccagc gcccttcgat
    22921 ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt cccagttatt
    22981 cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt ctttaccaag
    23041 atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct gatcgttgat
    23101 ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc gtgccggttt
    23161 agcattcagt tttgcctcac caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat
    23221 ggcaaagtag ttaatggtga tacgattttt ttgatccagc atcgcgccga gacggctgaa
    23281 tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca gggtgtccca
    23341 taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga caactttggt
    23401 ataagccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt ccgggttgag
    23461 ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg caaggtcgcc
    23521 tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt
    23581 ctccttaagt taactatccc ggtacttaag ccagggtata cttgtaattt tcttacggtg
    23641 cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac
    23701 ggtaaaagcg cgcaaagtgc ggcaaaactg ataaaaaaat catcgttttc cattgggtaa
    23761 aaatctgaca ctgatcatgt tatgaaaaaa aataacaact tttttatctg cttttgtcat
    23821 taacacagca cacaggcgta atatctgaca aaactgcatt tcgatttctt tcagagcgga
    23881 aatcgtcatt acccgtgagt ctctttacat catgaatatg ctggaaaaaa tccagtctca
    23941 gctggaacat ttgagcaaat ctgagcgcaa agttgccgag gtcattctgg cttcgcccga
    24001 taacgcgatc cattcgagta ttgctgctct ggcgctggaa gccaatgtta gcgaaccgac
    24061 ggtgaatcgt ttctgtcgca gcatggacac tcgcggtttt cctgatttta aacttcatct
    24121 ggcacaaagt ctggcgaatg gcactcccta tgttaatcgc aacgtcaatg aagatgacag
    24181 cgttgaatca tacacgggga aaatatttga gtccgcaatg gcaacgctcg atcatgtccg
    24241 tcattcactg gataaatctg ccatcaaccg cgccgtcgat ttgctcactc aggcaaaaaa
    24301 aatcgccttt ttcggattag gctcttcagc cgccgttgcc cacgatgcga tgaataagtt
    24361 ctttcgtttt aatgttccgg tggtgtactc cgatgatatc gtgctgcaac gcatgagttg
    24421 tatgaattgt agcgacggag acgtggtggt gctgatttct cacacgggaa gaacaaaaaa
    24481 tctggtcgag ctggcgcagc tggcacgcga aaacgacgcc atagtgattg ccctcacctc
    24541 tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt accctcgacg taccggaaga
    24601 tactgacatt tatatgccca tggtttctcg acttgcgcag ctgaccgtga tagatgtgct
    24661 ggcgacagga tttactttgc gacgcggtgc aaaattcaga gataacttga agcgggtcaa
    24721 ggaagcgctg aaggaatcgc gttttgataa gcagctactt aatttaagtg acgatcgcta
    24781 aaaacgactg tcactgtcct aatcttatac gacatccgaa tgagattaat ttatcgccat
    24841 cgcggcgtta tttcattcgg atttcatgtt caagcaacac ctggttgttt cagtcaacgg
    24901 agtattacat gtccagaagg cttcgcagaa caaaaatcgt taccacgtta ggcccagcaa
    24961 cagatcgcga taataacctt gaaaaagtta tcgcagcggg tgccaacgtt gtacgtatga
    25021 acttttctca cggctcgcct gaagatcaca aaatgcgcgc ggataaagtt cgtgaaattg
    25081 ccgcaaaact ggggcgtcat gtggctattc tgggtgacct ccaggggccc aaaattcgtg
    25141 tatccacctt taaagaaggc aaagttttcc tcaatattgg ggataaattc ctgctcgacg
    25201 ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc
    25261 ctgctgacgt cgtgcctggt gacatcctgc tgctggacga tggtcgcgtt cagttaaaag
    25321 tactggaagt tcagggcatg aaagtgttca ccgaagtgac cgtcggtggt cccctctcca
    25381 acaataaagg tatcaacaaa cttggcggcg gtttgtcggc tgaagcgctg accgaaaaag
    25441 acaaagcaga cattaagact gcagcgttaa ttggcgtaga ttacctggct gtctccttcc
    25501 cacgctgcgg cgaagatctg aactatgccc gtcggctggc acgcgatgca ggatgtgatg
    25561 cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg cagccaggat gcaatggatg
    25621 acatcatcct cgcctctgac gtggtaatgg ttgctcgcgg cgacctcggt gtggaaattg
    25681 gcgatccgga actggtcggc attcagaaag cgttgatccg tcgtgcgcgt cagcttaacc
    25741 gcgcagtaat cacggcgacc cagatgatgg agtcaatgat tactaacccg atgccgacgc
    25801 gtgcagaagt catggacgta gcaaacgccg ttctggatgg tactgacgct gtgatgcttt
    25861 ctgcagaaac tgctgctggt cagtatccgt cagaaaccgt tgcagccatg gcacgcgttt
    25921 gcctgggcgc ggaaaaaatc ccgagcatca acgtttctaa acaccgtctg gacgttcagt
    25981 tcgacaatgt ggaagaagcc attgccatgt cagcaatgta cgcggctaac cacctgaaag
    26041 gcgttacggc gatcatcacc atgaccgaat cgggtcgtac cgcgctgatg acctcccgta
    26101 tcagctctgg tctgccaatt ttcgccatgt cacgccatga acgtacgctg aacctgactg
    26161 ctctctatcg cggcgttacg ccggtgcact ttgatagcgc taatgacggc gtagcggctg
    26221 ccagcgaagc ggttaatctg ctgcgcgata aaggttactt gatgtctggt gacctggtga
    26281 ttgtcaccca gggcgacgtg atgagtaccg tgggttctac taataccacg cgtattttaa
    26341 cggtagagta agtacgttgc cggatgcggc gaaaacgcct catccggcct acagttcaat
    26401 gataattcaa cagatttcga atcttctgaa gcaaacttga acttatcacc agtcgaaggc
    26461 ctctcctcgc gagaggcatt ttttatttga tgggataaag atctttgcgc ttatacggtt
    26521 ggatttcgcc cggtttgcga gttttcagca attttaatat ccaggtgtat tgttctggtc
    26581 gcggaccaac aaaaatctcg acttcttcat tcatccgccg cgcaatcgta tgatcatccg
    26641 cctctaacag atcatccatc ggtgggcgca cctgaatcgt caaacgatgc gttttgccat
    26701 cataaatcgg aaacagcggt acaacgcgcg cacggcacac tttcatcaaa cgaccaatcg
    26761 caggcaacgt cgctttatag gtggcaaaga aatcaacaaa ttcgctgtgc tctgggccat
    26821 gatcctgatc gggtaaataa tatccccagt agccctgacg taccgactgg atgaatggtt
    26881 taataccatc atttctcgca tgcagacgac cgccaaagcg acgacgcacc gtgttccaga
    26941 cataatcaaa aaccgggttg ccctgattat ggaacatcgc tgccattttc tgcccttgcg
    27001 aggccatcag catggcagga atatcgacgg cccaaccgtg cggcaccaga aaaatcactt
    27061 tctcgttatt acgccgcatc tcttcgataa tctccagccc ttgccagtca acgcgcggct
    27121 gaattttctc cggcccgcgt attgccaact cagccatcat tgccatcgct tgcggcgcgg
    27181 tggcaaacat ctcatctaca atcgcttcgc gttcagcttc actacgttct ggaaagcaga
    27241 gcgacagatt gattaacgca cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc
    27301 ccagccgtgc cagaatggga tcacggaact ttggcggcgt taaagcgata cccgccatcg
    27361 cggctacgcc cagccatgct ccccagtagc gcgggtggcg aaaggattta tcaaactcag
    27421 gaatgtattc gctattattt tttttcgttt ccatgctttt ccagtttcgg ataaggcaaa
    27481 aatcaatctg gtgatagtgt agcggcgcaa cttgccccgc accaaataaa aaaagccggt
    27541 actgactgcg taccggctgc gaatggatgt taattaatca aaccgtagct gcggcacaat
    27601 ctctttggcc tgcgccagga attcgcgacg atcggagccg gtcagccctt cggtacgcgg
    27661 cagttttgcc gtcagcgggt ttacggcctg ctggtttatc catacttcat agtgcagatg
    27721 cggcccggtt gaacgtccgg tattaccgga aagcgcgata cggtcgccac gtttcacctt
    27781 ctgtcccggt ttcaccagga tcttgcgcaa gtgcatataa cgcgtggtgt agctgcgacc
    27841 atgacgaata gccacataat aacctgctgc gccactgcgt ttggcaacca ccacttcacc
    27901 gtcacccact gaaagcactg gcgtaccttg tggcatggca aaatcaacac ctctgtgtgg
    27961 cgcaacgcga ccggtcaccg gattagtacg acgcgggtta aagttagatg agatacggaa
    28021 ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga ccagtaccgt tacgatcgta
    28081 gaatttgcca tcttcagcgc ggattgcgta ataatcttta ccttctgaac gcaaacgtac
    28141 gcccagcagc tggctttgct cacgtttacc atcaagcatt tctcgtgaca ttaacaccgc
    28201 aaattcatcg ccttttttca gtttgcggaa atccatttgc cactgcatgg ctttaatcac
    28261 tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg ctggcaacaa agcttccccc
    28321 gacggtacct ttcagcagat tgttgaccca ctctccttgc tgcatttcgc tggtcatttt
    28381 aaaaccgtta gcggcagtac ggtcataggt tcgggtttca cgacgagaca cttcccaggt
    28441 gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag agttgttgac cgattttcag
    28501 gttacgcaat tctttgtcgg cagcagccag ttgggtgata tcacccatat caataccata
    28561 ctgattgaga atgctgctta gcgtatcgcc agtggaaaca acatattcat gcacgcccgc
    28621 ttcaccggcg attttgtcat ccagttcatc ctggggaatg gcttcatctt cttgtgcagc
    28681 ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga atttcgttct gttccagctc
    28741 aatggttttg acaattggcg tggcatcgcg gtgataaaca tagggccgcc agacagcgac
    28801 ggccagagta agaacggtga gcgaccctaa cataacgcgg tgtggtcgcg gtaaattatt
    28861 aaacgccagg gcgacagagc gggctatctg ttgcacgtaa tcacttcctc attaatctcc
    28921 tttcaggcag ctcgcatact ggttggctaa ttgattcagg aattctgaat agcttgtttt
    28981 acccagtttg atattcgtcc ccaggggatc caacgttccc atacgaacgg atgtccctcg
    29041 tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca gcaaaaacgc aggttgcttt
    29101 ttgctcaacc aactgtgttc ttatttcatg taaacgctgc gcgccaggtt gaatctcagg
    29161 gttaacggta aaatgaccaa gcggtgtcag tccgaactgt ttttcgaagt agccgtaagc
    29221 atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg agctcgttac caacctgcgt
    29281 ttcggttgag gctaattgtg cctcaaaatc cttcaggttg gcgtcaagtt tggctcgact
    29341 ttgcggcata agttccacta attttccatg gattgcaacc gctgtagccc gcgctatctc
    29401 tggggaaagc caaagatgca tgttgaaatc gccgtgatgg tgatcttcgt cacttttttc
    29461 cgcgtggtcg tgatcatcat catcgtcgtg aatacttttc atcagcagcg gtttcacatc
    29521 ttctagctgc gcaatcgtta cctgtttcgc ttctggtaat ttacttaccg gtttttgcat
    29581 gaacgcttcc atctccgggc caacccaaac gactaagtcc gcgttctgta agcgttttac
    29641 atccgatgga cgcagtgaat aatcatgttc tgaagcgccg tcaggcagta aaacctgcgt
    29701 ttctgttacc ccatcagcaa tggcagaagc gatgaaccca acgggtttaa gcgaagcgac
    29761 aacggcggca tctgcggcct gtgttgcacc gccccagaga gcggcggata atgctgcgaa
    29821 aagaagcgtt tttttatgta acataatgcg accaatcatc gtaatgaata tgagaactgt
    29881 gatattataa catttcatga ctactgcaag actaaaatta acatgacaag tctggtttcc
    29941 ctggaaaatg tctcggtttc ttttggccaa cgccgcgtcc tctctgatgt gtcgctggaa
    30001 cttaaacctg gaaaaatttt gactttactt gggccaaacg gcgcaggtaa gtcgacactg
    30061 gtacgggtag tgctcgggct ggtaacaccc gatgaagggg ttatcaagcg caacggaaaa
    30121 ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca ccacgttgcc actgaccgta
    30181 aaccgttttt tacgcttacg ccctggcaca cataaagaag atattttgcc tgcactgaaa
    30241 cgtgtccagg ccgggcatct gattaacgca ccgatgcaaa agctctcggg tggcgaaacg
    30301 cagcgtgtac tgttagcgcg agcattgtta aatcgaccgc aattattagt gctggatgaa
    30361 cccactcagg gcgtggatgt gaatggtcag gtggcgttat atgaccttat tgaccaactg
    30421 cgtcacgaac tggattgtgg cgttttaatg gtatctcacg atctgcatct ggtaatggca
    30481 aaaaccgatg aagtgctttg cctgaatcac cacatttgtt gttccggcac accggaagtt
    30541 gtttccctgc atccggagtt tatttctatg tttggtcctc gtggtgctga acaactgggt
    30601 atctatcgcc atcatcataa tcatcgtcac gatttacagg gacgaattgt tttgcgtcgg
    30661 ggaaatgatc gctcatgatt gaattattat ttcccggttg gttagccggg atcatgctcg
    30721 cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg tcgtatgtct tatttcggtg
    30781 atacgctggc tcatgcctca ttacttggcg tcgcgtttgg tttgttgctg gacgtgaatc
    30841 cattctatgc ggtgattgca gttacgctgc tgctggcggg cggtctggta tggctggaga
    30901 agcgtccaca gctggcgatc gacacgttat tagggattat ggcgcacagt gccctgtcgc
    30961 tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt tgatttgatg gcttacctgt
    31021 tcggtgattt actggcagtg acgccagaag atctcatctc tattgcgatt ggcgtggtca
    31081 tcgtggtggc tattatgttc tggcaatggc gcaatttgct gtcgatgacg attagcccgg
    31141 atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa attgttgttg atgctggtga
    31201 cggcattgac gattggtgta gcgatgaaat ttgtcggcgc gttgattatt acttcactgc
    31261 tgattattcc tgctgctact gcacgtcgct ttgcccgcac gccggaacag atggctggtg
    31321 tcgctgtttt ggtggggatg gtggcagtga ctggcggttt aaccttttcc gcattttacg
    31381 atacacctgc aggcccgtcg gtggtgctat gtgcggcact gttatttatt atcagtatga
    31441 tgaaaaagca ggccagctaa tctatcgctg aacacatttg tcggatgcgg cgcgagcgcc
    31501 ttatccgacc tatggttcgc tatctctggt atatgcctga taagacgcga atagcggcgc
    31561 atcaggcaac actgccagtg tcggatgcgc cgcgagcgac cgatccgact tacggcattt
    31621 ctggcggcgt gatgccgaag tggttccacg cccgcactgt cgccatacgc ccgcgcggtg
    31681 tacgctgcaa aaagccttgc tgaatcaaat aaggttccag cacatcctca atggtttcac
    31741 gttcttcgcc aatggcagcc gccaggttat ccagacctac cggcccacca aagaacttat
    31801 cgattaccgc cagcaacaat ttgcggtcca tataatcgaa accttcggca tcgacattca
    31861 gcatatccag cgcctgagca gcgatatcag ccgagatggt gccatcgtgc ttcacttcgg
    31921 cgaaatcacg cactcgacgc agcagacggt tagcaatgcg cggcgtaccg cgagcgcgac
    31981 gagcaacttc cagcgcgccg tcatcactca tctcaagccc cataaagcgt gcgctgcgac
    32041 tgacgatata ttgcagatcc ggcacctgat aaaactccag acgctgcaca ataccaaaac
    32101 gatcgcgcaa cggtgatgtc agcgaacctg cgcgcgtggt tgcgccaatc agtgtaaacg
    32161 gcggcaaatc aattttaatg gagcgtgccg ccggaccttc accaatcatg atatccagtt
    32221 ggtagtcttc cattgccgga tacaacacct cttccaccac tggtgaaaga cggtggatct
    32281 catcaataaa cagtacatcg tgtggttcaa ggttagtgag cattgctgcc agatcgcccg
    32341 ccttttccag caccggacca gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa
    32401 tattggcaag cgtagtttta cccaaccccg gaggaccaaa aatcaacaga tgatcgaggg
    32461 catcgccgcg cagtttcgct gctttgatga aaatctccat ctgtgaacga acctgcggtt
    32521 gaccaacata ctcttccagt aatttagggc gaatggcgcg atctgccaca tcttccggca
    32581 aagtggtacc ggcagaaatc agacggtctg cttcaatcat cctttacctc ataacgcggc
    32641 gcgtagggct tcgcgaatta atgtttcact gctggcgtca gggcgagcga ttttgctcac
    32701 catacggctt gcttcttgtg gtttatagcc cagcgccacc agcgcagcaa ccgcttcctg
    32761 ttcagcatcg tcggtcgccg ggctggcagg tgacgtgagt accaggtcgg cggctggcgt
    32821 aaagagatcg ccatgcaaac ctttaaatcg gtctttcatt tcgacaatca agcgttcggc
    32881 ggtttttttg ccaatacccg gcagtttcac cagtgccccc acttcttcac gctcaacggc
    32941 attaacgaac tgctgcgctg acattccgga gaggatcgcc agcgccaact tcgggccgac
    33001 gccgttggtt ttgatcaact ctttgaacaa cgtgcgctct tgtttattgt taaaaccgta
    33061 cagcagttgc gcgtcttcac gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc
    33121 ttcagggagt tcataaaaac aggtcatcgg catatgcact tcatagccta cgccgcccac
    33181 ttcaattaac accagcgggg gttgtttttc aatgatgatg cctctgagtc tgcctatcac
    33241 atgacgctcc tgcgtaatga atcaaagata atgctgtatg ataaaaaaat gctggataga
    33301 tatccagcga aggatgaaga aaacttgcga ggtgtcccga tgatctgaaa aatggcgcag
    33361 tataatttat tctacagatt atattggaag caaatattta aatattacat attcagcgaa
    33421 gaaatgtgta ataaaaatac acatcgcgac ccctgaaaaa ataaattttt tatgctatta
    33481 cgtatattca tatctatttc aatggaatga caacgtgaat gttaattatc ctgctgaata
    33541 tgaaattggt gatatcgtct ttacatgtat aggtgctacc ttatttggtc aaatatcagc
    33601 tgcatcaaat tgctggagta atcacgtcgg gatcattatc ggtcataacg gtgaagactt
    33661 cctggttgca gaaagccggg ttcccctttc aaccatcacc acgctatctc gttttattaa
    33721 acgttcgtct aatcaacgct atgctataaa gcgattagac gctgggctga cagaacaaca
    33781 aaaacaacga attgttgaac aggttccctc ccggctacgc aaactttacc acaccggttt
    33841 taaatacgaa tcttcgcgcc agttctgttc aaaatttgtt tttgatattt ataaagaagc
    33901 gctatgtatt ccggtgggtg aaatagagac gtttggagaa ttgttaaata gcaatccgaa
    33961 tgcaaaactc actttctgga aattctggtt cttaggttct attccgtggg agcgtaaaac
    34021 cgtcacgcca gccagtttgt ggcatcatcc gggtttggtg ttgattcatg cggagggagt
    34081 ggaaacgcct cagccggaac tgaccgaggc ggtataactt aacgcagtcg ccctctcgcc
    34141 aggttcagcc gcgattcgct catctgcatc gcattctgac tgacgtgaca atgggtaatc
    34201 gcgatagcta atgcatcggc agcatccgcc tgtggattag cgggcagttt cagcaaggtg
    34261 cggaccatat gctgcacctg gcttttttcg gcactaccaa tacctaccac cgtttgcttt
    34321 acctgacgtg ccgcatattc aaataccggc aattcctgat tcaccgctgc cacaatcgcc
    34381 acgccgcgcg cctgacccag tttcagggct gagtcagcgt tcttcgccat aaagacttgt
    34441 tcaatggcga aataatcagg ctggaactgg gtgatgattt ccgtcacgcc cgcatagatg
    34501 agcttcagac gagacggtaa atcatccact ttggtgcgta tgcatccgct acccaggtag
    34561 gacagttgcc tgcctacctg gcggatgacg ccatagccgg tcacgcgcga acccgggtca
    34621 atgccgagaa taatagccat cacgcgtctc cgttttgctg tttagcaggc ctcatcagag
    34681 agtcgctgca acctcatcag agatttcacc gttatggtaa acttcctgca cgtcgtcgca
    34741 atcttccagc atatcgatca gacgcatcag tttcggtgcg gtttctgcat ccatatcagc
    34801 tttggtggac gggatcatgg aaacttccgc gctgtctgct ttcagacctg ccgcttccag
    34861 agcgtcgcgt actttgccca tttcttccca tgcagtgtag acatcaatcg cgccgtcatc
    34921 ataggtcaca acgtcttcag caccggcttc cagggctgct tccatgatgg tgtcttcatc
    34981 gcctttctcg aaggagatca cgcctttttt gctgaacaga taagctacgg aaccatcagt
    35041 accgaggtta ccgccacatt tactgaatgc atggcgcact tcagcaacgg tacggttgcg
    35101 gttgtcagac agacattcaa tcatgattgc cgtgccgcca ggaccgtaac cttcgtagat
    35161 gatggtttcc atgtttgcat catcatcacc gcccacacct cgtgcaattg cgcggttcag
    35221 agtgtcacgg gtcatgttgt tagacagtgc tttatcaatt gctgcacgca gacgcgggtt
    35281 agcgtccgga tcaccaccgc ccagcttagc cgcggttacc agctcacgaa tgattttagt
    35341 gaagatttta ccgcgcttag catcctgcgc agctttacga tgtctggtgt tggcccattt
    35401 actatgacct gccataaaaa tatctccaga tagccctgcc tgttcaggca gcgttaatta
    35461 caaactgttc aatcgcctgc cggttgctcc aggacttagt gagcgccgca gcagcaggcg
    35521 cgtcaagcca cttgtaagcc agatgttcag tgaaaacgat ctggcgctcg tgcgggagcg
    35581 caagacagaa ccatgattcc gtattacgcg tcacgcccgg cgcatagcga tgacgtaaat
    35641 gtgaaaaaat ttcaaactct accgtgcgct gacagtcaat taaggtcagt tgttcagcga
    35701 caacatcaat ggtgacctct tcctttactt cgcgcatggc agcttgcggc gcggtttcac
    35761 cctcttccac gctgccggtt accgactgcc agaaatcggg atcgtcacgc cgctgcaaca
    35821 tcagcacccg tttcgtatct tgtgcataga tgaccactaa gatcgaaacg ggacgcttat
    35881 acactttatc cttcacgctg cctcttcgtt gactgccttc gctcatccca ttcacatagt
    35941 tatctatgct catgggagtt cactcagttg ccgcctcgat gcaacgcgaa tgatttcgtg
    36001 tatttgagtc atatcagtta ttctcgtcct tcttcacaac ctgaatgctc agctcagcca
    36061 gtgcagtcgg gttagcaaag ctcggtgctt cagtcatcag acacgctgcc gccgtggttt
    36121 tcgggaaggc gataacgtca cggatattgt cggtgccggt cagcagcatg gtcagacggt
    36181 caagaccgaa tgccagacct gcgtgcggcg gagtaccgta tttcagggcg tcgagcagga
    36241 agccgaattt ctcgcgctgt tcctcttcgt tgatacccag aataccaaac accgtctgct
    36301 gcatatcacc attatggata cgcacagaac caccgcccac ttcgtaacca ttgatgacca
    36361 tatcgtaagc gttcgccacc gcattttctg gtgcagcttt cagttctgca gcagtcatgt
    36421 ctttcggtga ggtgaacgga tggtgcattg ccgtcaggcc gccttcaccg tcgtcttcaa
    36481 acatcgggaa gtcgataacc cacagcggtg cccatttgct ttcgtcggtc agaccaaggt
    36541 ctttacccac tttcaggcgc agtgcgccca tcgcgtcggc aacaattttc ttgttgtcgg
    36601 caccgaagaa aatcatatcg ccatcttgcg cggcagtacg atccaggatg gcttcgatga
    36661 tttctgcatt aaggaacttc gctaccgggc tgttgatacc ttccagacct ttcgcgcgtt
    36721 cgttaacttt gatgtaagcc agacctttcg cgccgtagat tttaacgaag ttaccgtatt
    36781 cgtcgatctg cttacgggtc aacgatgcgc cgcccggaac acgcagagcg gcaacacggc
    36841 ctttcggatc gttcgccgga cctgcaaata ctgcaaactc aacagatttc agcagatcgg
    36901 caacgtcggt cagttccatc gggttacgca gatccggttt atcagaacca taacggcgtt
    36961 ctgcttctgc aaaggtcatt accgggaaat cgcccagatc cacgcccttc acttccagcc
    37021 acagatgacg caccagcgct tccatcactt cacgcacttg cggcgcggtc atgaaagaag
    37081 tttccacatc gatctgagta aattcaggct gacggtcagc acgcaggtct tcgtcacgga
    37141 agcatttaac gatctgatag tagcggtcaa agccggacat catcagtagc tgtttgaaca
    37201 actgcgggga ttgcggcagc gcgtagaatt tacctttgtg cacacgagaa ggcaccaggt
    37261 agtcacgcgc gccttccggc gtggctttgg tcagcatcgg agtttcgatg tcgaggaagc
    37321 cgtggtcatc cataaaacgg cgcaccaggc tggtgatttt agcgcgggtt ttcaggcgct
    37381 gagccatttc cgggcgacgc aggtcgaggt agcggtattt cagacgcgct tcttcggtgt
    37441 tgacgtggtt agagtcaagc ggcagaacat ctgcacgatt gatgatagtc agcgaggacg
    37501 ccagtacttc gatttcgcca gtcgccatat cgcggttaat atttttttcg tcacgcgcac
    37561 gtacggtgcc cgtgacctga atgcagaact cattacgcag ttcagaggcc agctttaacg
    37621 cgtccgcacg atccggatcg aaaaatacct gcacgatacc ttcgcggtcg cgcatatcga
    37681 tgaagatcag gctaccaaga tcacgacgac ggttgaccca accacacaga gtcacctgct
    37741 gccccacgtg ggacaaacgg agctgtccac aatattctgt acgcatgaga tatcccttaa
    37801 cttagctgcc ggcggatgcc ccctgctgcg caggtgacca agtcgcagcg ttagctgtat
    37861 gtcacaactg aatgaaaaaa ggcggctatt atactggaaa ttctgccgca ccgtaagagc
    37921 ctggcccgcg ctggaacgcc tcgttaccac tttatatcgg gcctgaaatc agactctacg
    37981 ccagtttgct ataaaggtgt tgcccgaact cataaaaatt aacaaaattt gtcgttccgc
    38041 catcggctaa tcgcattaag gtgagaggca cgattttgtt ttgtcaggag tcatcatgct
    38101 tgaacttaat gctaaaacca ccgcgctggt ggtgattgat ttacaagaag gcatcttacc
    38161 ttttgccgga ggtccacata ctgccgatga ggtggttaat cgcgccggga agctggcggc
    38221 gaaatttcgc gccagcggtc agcccgtgtt tctggtgcgc gttggctggt ctgccgatta
    38281 cgccgaagca ttaaaacagc cggttgatgc cccctccccc gcaaaagtgt tgcccgaaaa
    38341 ctggtggcaa catcctgctg cattaggtgc aaccgacagc gatatcgaaa tcatcaaacg
    38401 tcaatggggt gcgttttacg gtacggatct ggagttgcaa ttacgccgcc ggggtatcga
    38461 tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt gaatccaccg cccgcaatgc
    38521 ctgggaactc ggttttaatc tggtgattgc cgaagatgcc tgtagcgccg ccagcgccga
    38581 gcagcacaat aacagcatta atcatatcta cccgcgcatc gcccgtgtgc gcagtgtgga
    38641 agagatcctc cacgcgttat gatttacatc ggtctgccgc aatggtcgca tcctaaatgg
    38701 gtgcggttgg ggatcaccaa ccttgaagag tatgcccgcc actttaactg cgtggagggt
    38761 aacaccacgc tttatgccct gccgaaaccc gaggttgtcc tgcgctggcg tgagcagacc
    38821 acagatgact tccgcttctg ttttaagttt ccggcgacca tttcgcatca ggcagcatta
    38881 cggcattgcg atgatttagt gactgaattt ttgacccgca tgtcaccgtt ggctccgcgc
    38941 attggacaat actggctgca actgcctgcc acattcggcc cacgagagct gcctgcgctt
    39001 tggcattttc tcgattctct tcccggtgaa tttaattatg gggtggaagt ccgccatcca
    39061 cagtttttcg ccaaagggga agaggaacaa acgcttaatc gcggtttaca tcagcgcggc
    39121 gttaatcggg tgattttaga cagccgcccg gttcatgcag cacgtccaca cagtgaagct
    39181 attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac atgctgtact gacggcgaaa
    39241 aatccactga tccgttttat cggtagtgat gatatgacgc aaaaccggga gttatttcag
    39301 gtctggttac aaaaattagc acagtggcat cagaccacta cgccttatct ttttttacat
    39361 acgccagata ttgcccaggc cccggaactg gtacataccc tgtgggaaga cttacgtaaa
    39421 acgcttccag agatcggagc agttccggct attccacagc aatcttctct tttctgaatt
    39481 tgccacctat catagacagg tgccatcggc cattttaaag ggagtttgta tggtaagcgc
    39541 gctgtatgcc gttttaagtg cgttgttatt aatgaagttc tcttttgatg tcgttcgcct
    39601 gcgaatgcag taccgcgttg cctatggcga cggcggtttt agcgaactgc aaagcgctat
    39661 tcgcattcat ggtaacgcgg tggaatatat tcctatcgcg attgtgttga tgctgtttat
    39721 ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc ggcatcgttt tgcttgctgg
    39781 tcgtctgatg cattattacg gttttcatca ccgtctgttc cgctggcgac gttctggcat
    39841 gagcgcgacc tggtgtgcgc tgttgctgat ggtgctggcg aatctttggt atatgccctg
    39901 ggagttggtt ttctccctgc gttagcgcac aatacgccac tttctttttc ccggattttt
    39961 acgttatgtc tcaccgcgac acgctatttt ctgcccctat cgccagactg ggcgactgga
    40021 cctttgatga acgggtagct gaagtcttcc cggatatgat ccagcgttcc gttcccggct
    40081 attccaatat tatttccatg attggtatgt tagccgagcg cttcgttcaa cctggtacgc
    40141 aggtttacga tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc
    40201 atcatgataa ttgcaaaatt attgccatcg ataactcccc cgcgatgatt gaacgctgcc
    40261 gtcgtcatat tgacgcctat aaagccccta cgccagtaga cgtcattgaa ggtgatattc
    40321 gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg caattcctgg
    40381 aaccttccga gcgccaggcg ttactggata aaatttatca agggctgaac ccgggcggcg
    40441 cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa ctgttgttca
    40501 acatgcacca cgactttaaa cgtgccaacg gttacagcga actggagatc agccagaagc
    40561 gcagcatgct ggaaaacgtg atgctgaccg attccgtgga aacccataaa gcacgcctgc
    40621 ataaagctgg ttttgaacat agtgagctgt ggttccagtg ctttaacttt ggttcactgg
    40681 tggcattaaa agcagaggac gctgcatgat cgactttggt aacttttatt ctctgattgc
    40741 caaaaatcat ctttcacact ggctggaaac gctgcccgcg cagattgcta actggcagcg
    40801 cgagcagcag cacgggctgt ttaagcagtg gtccaacgcg gtggaatttc tgcctgaaat
    40861 taaaccgtat cgtctggatt tattgcatag cgtaaccgcc gaaagcgaag agccactgag
    40921 cgccgggcaa attaaacgca ttgaaacgct gatgcgcaac ctgatgccgt ggcgcaaagg
    40981 gccgttctca ctgtatggcg tcaacatcga taccgaatgg cgttccgact ggaaatggga
    41041 tcgcgttctg ccccatcttt ctgatttaac cgggcgcacc attcttgatg tcggctgtgg
    41101 cagcggttat cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg tgggaatcga
    41161 ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt aaactactgg gtaacgatca
    41221 gcgcgcacat ttgttaccgt taggtattga acagcttccg gcattgaaag cctttgatac
    41281 cgtcttttcg atgggcgtgc tttatcatcg tcgttcaccg ctggagcatc tctggcagtt
    41341 aaaagaccaa ctggtgaatg aaggtgaact ggtgctggaa acgctggtta ttgatggtga
    41401 tgagaacacg gtgctggtgc cgggcgatcg ttacgcgcaa atgcgtaatg tctatttcat
    41461 cccttccgcg ctggcgctga aaaactggct gaagaagtgt ggttttgtag atatccgcat
    41521 tgcggatgtg agcgttacca ccacagaaga gcagcgacgc accgaatgga tggtcaccga
    41581 gtctctggcc gattttctcg acccgcatga tccgggtaaa acggtggaag gttatcctgc
    41641 gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg tctggtatta ctgccggatg
    41701 cggcgtgaac gccttatccg gcctacaaaa ccttgctaat tcaatatatt gcagggacta
    41761 tgtaggcctt ataagcatag cgcatcaggc agttttacgt ttgcataacc tcagcgcccg
    41821 tttccgggcg cttttcacgt cttatgcctg aacagctggc tgatcaaacg ccgttaactt
    41881 cggtgcattg cccgtatatt tttcgatata caccagcgca gagttaccgg cacaggcatt
    41941 tgccagctga gagctgggga tatccgccgt taacacgttc gcactgccgt ttttacacaa
    42001 gccattttcc agatctggcc atgcaccttc atgcacgcat accacgcctt ttttgatccc
    42061 gtcagtcacc accgcgcctg tcagaatctg accacgtttg ttccagacac gcaccagatc
    42121 gccattcgca ataccaaagc gagcagcatc ttcggtgtga atagtgatcg gttcacgatc
    42181 ggcaaccgca tattttttac gcagttccgc atagttaagc tgactgtgta aacggtgtgc
    42241 cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg gaacctttcc actcatcagg
    42301 cgcaagccag gttgggtgtg ccgggcaatc cttatagcca aatttttcca gcgttttgga
    42361 gtaaatctca attttgccgc ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa
    42421 atcaccataa cgaacgtatt gttcgttctt ctcactgcgg cgcatttcga tcagtttatt
    42481 ttgctgccag aaagcattaa acatcggcat agtcacgcgt tgcgcacggg cacctttctg
    42541 agcggcatca tagaaaaatt tcagccacgc catttcatct ttaccttcgg tatagatctc
    42601 ttttccgcca ggtttgagta attcagcaag atcggcaaac acgtcaaaat cgttgcgcgc
    42661 ttcaaattgc ggagcgacag cctgcttcat cggcacaata tgctggttgc tgtaatcacc
    42721 ggtcatcgtc aggtcattgc gctcaaacga tgtggtgatc ggtaatacga tatctgcatg
    42781 tttagcagct gcggtccagt agcattcaga aacgacgatc atctccggtt tctgccatgc
    42841 tttaatcaga cgattagtat cctggtgatg ggtaaagtta ccgccacccg cccaccagat
    42901 cattttgata ttcggatacg tttgttcttt accgttatgc tgatatttgc cgccaggatt
    42961 ttccagcgca tcgacgatac gggcaacagg aatcgccgtc attccgccat catcagcggc
    43021 ctcgctggcc tgtccggcaa tcgccgcaga catttccggc agcacgccgc caacacgcgt
    43081 cgggttgcca ccgttggagt aatgataaga gaatccaaaa ccgccgcccg gtgtaccaat
    43141 ttgccctaac atcgccgcca gcgtcaccag catccagtgt ttctgttcac cgtattgctg
    43201 gcgttggatc ccccagcctg ccatcagcat cgtgcgattc gccgccatca gttctgcaag
    43261 tttgacaatc tgcgcttccg gtacgccagt aatttccgcc gcccaggccg cgctcttcgg
    43321 cgtattatcg ctcttacctg tcagatactc ttcaaactgc ggatacccgg tagtgtattt
    43381 ttcaaggaac actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa tccctaacat
    43441 cagtgccacg tcggtgccca tattcggtgc gatccaggtg gcattatcgc caaagaattc
    43501 gatggtttcg gagcagatag gatcaatggc aatcactggt ttgccagatt ttttcagctg
    43561 atggaagtat tccagccctt gctcatcggt actgctccag gcaattttga gcgtgttcag
    43621 cgggttcatc ccccacagca ccacaacctg gctgttttcc agaatcagcg gccaggaggt
    43681 ctgctgttca tacacctcta cagaaccgac cacatgcggc atgatcacct gtgccgctcc
    43741 ggttgaataa tcgccgctat gcccggaata accgcccgcc aggttcatat aacgttgcag
    43801 taaggtttgc gctttatgca acacgccaga agaacgccag ccgtaagatc cggcaaaaat
    43861 ggccgatggt ccgttagctt tacgaatacg atcgtgttgc tcatgaatca gttttaatgc
    43921 ttgttcccag ctcacctgta cataggtatc ttcgccacga cctttcgccg gttgcagtgg
    43981 attatcgaga tagctttttc tcaccatcgg atgctgaata cgcgccgtgg tgtgtacctg
    44041 atccgccgcc gtagactgta aggagttcgg tatggttttc gccagcgcgc ctgtcgaaga
    44101 aacaatcttg ccgtccttca cttctacgtt catcgctccc caacggcccg cggtgagaat
    44161 tttgccgccc ttctcttctg cccatgcagg taacggtgca gccgatgtca ccaccagcgc
    44221 ccctgccgca ataccgctat gtttaataaa ttcacgtctt gttaatgtca taacttcctc
    44281 cctgatcaac gaggatcact gtttctcggt aatatctttg gcgttgtact ggaagtaccg
    44341 cgtcaaaatg tccagttcgt tttcgctcat gctggttcgc gcacccattc ctttggcaat
    44401 ggacggccat gcattgacgg tgtaatggtc ggaggcaata ggggcatggc aaccagcgca
    44461 ataggtatcg tcaagttttt cagcgtattg ccatagaggt ttacggtccg ctaatgcggg
    44521 atcggtaagc acaccctgta aagacgcctg acgccattga ttgccgtatt cgtcagcctg
    44581 ccattccccg tttaccgtca gcgccttgat accttcttca cttaatgtgg ctagcgccag
    44641 ccgttgacct gccgccaggt agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa
    44701 cagaacgatc ggctgtttgc cactggcatc aacgacggtg agatcggtac caggattcac
    44761 ggtagccagt tcgcctatgc gagaagtttt gaaaggataa atatgtgcgc cattagtaac
    44821 tgaagtagcg gcctgacttt ccagctcatg cgccgcgttg tcatccattt ttatttctgg
    44881 cggaaaatgg gcaatgcctt tatggcaatc gatacaggtt tcgccgtcct tttgcgcttt
    44941 attatgcatt ttctgcgcag attcactttg cgaggcaata tccatggcat caaaagaatg
    45001 gcaactacgg caggttgcag agtcagtggc ttttaattct ttccatactg tttcggccat
    45061 ttcctggcga tgagcttcga acttatcatc actgtctatt ttgccgctaa caaattcatg
    45121 ataaatatct ttagatgctt ttaatttagc aaataaataa tccatccctg actttggaat
    45181 atggcaatcg gcacattccg cacgtatccc tttctggttt gaaaagtgga cagttccctg
    45241 atattcctca aaaggtttac tcatcgagtg gcaggaaagg caaaatgcgg tatccgacgt
    45301 tttatgtaag gctttttgcg ccagtaataa cccgccgcca ccaaccacaa ccgctatcag
    45361 caaaaacaat aacccaatgc gttttttccc tcgcatagat tcctctaatt aaattccagg
    45421 acaccgatac cattgtataa ataaatacac aacgaattga ctacttgctt aattcggcac
    45481 aacactgcct gaaataatcg ataaagaata tgatttatta caatgtaatc attaattgct
    45541 aaggaataac ctagttgtta tttagaatat atatttgtca gttttggcaa tttaaatcac
    45601 ataatgaatg tgaatatata aaatattttt agcacaacgc tatgcgcact gtcacccgct
    45661 caacagatca cggcaatcgt tcgtttttta tactgctcag ag
//
LOCUS       DAZGTJ010000030        43393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000030 DAZGTJ010000000
VERSION     DAZGTJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.030"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..177)
                     /locus_tag="ACHK8E_002114"
     CDS             complement(<1..177)
                     /locus_tag="ACHK8E_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344386.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLNYKLTKRIYF"
     gene            255..1349
                     /gene="ddlA"
                     /locus_tag="ACHK8E_002115"
     CDS             255..1349
                     /gene="ddlA"
                     /locus_tag="ACHK8E_002115"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0344387.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1552..1764)
                     /gene="yaiZ"
                     /locus_tag="ACHK8E_002116"
     CDS             complement(1552..1764)
                     /gene="yaiZ"
                     /locus_tag="ACHK8E_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP0344388.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            2024..2332
                     /gene="yaiY"
                     /locus_tag="ACHK8E_002117"
     CDS             2024..2332
                     /gene="yaiY"
                     /locus_tag="ACHK8E_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP0344389.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2391..3485)
                     /gene="yaiW"
                     /locus_tag="ACHK8E_002118"
     CDS             complement(2391..3485)
                     /gene="yaiW"
                     /locus_tag="ACHK8E_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP0344390.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3498..4718)
                     /gene="sbmA"
                     /locus_tag="ACHK8E_002119"
                     /pseudo
     CDS             complement(3498..4718)
                     /gene="sbmA"
                     /locus_tag="ACHK8E_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5070..6227
                     /gene="ampH"
                     /locus_tag="ACHK8E_002120"
                     /pseudo
     CDS             5070..6227
                     /gene="ampH"
                     /locus_tag="ACHK8E_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
     gene            complement(6228..6851)
                     /gene="iprA"
                     /locus_tag="ACHK8E_002121"
     CDS             complement(6228..6851)
                     /gene="iprA"
                     /locus_tag="ACHK8E_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP0344391.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6937..9885)
                     /gene="ehaB"
                     /locus_tag="ACHK8E_002122"
     CDS             complement(6937..9885)
                     /gene="ehaB"
                     /locus_tag="ACHK8E_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP0344392.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            10411..11385
                     /gene="hemB"
                     /locus_tag="ACHK8E_002123"
     CDS             10411..11385
                     /gene="hemB"
                     /locus_tag="ACHK8E_002123"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0344393.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11425..12276)
                     /gene="tauD"
                     /locus_tag="ACHK8E_002124"
     CDS             complement(11425..12276)
                     /gene="tauD"
                     /locus_tag="ACHK8E_002124"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP0344394.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12273..13100)
                     /gene="tauC"
                     /locus_tag="ACHK8E_002125"
     CDS             complement(12273..13100)
                     /gene="tauC"
                     /locus_tag="ACHK8E_002125"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP0344395.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(13097..13864)
                     /gene="tauB"
                     /locus_tag="ACHK8E_002126"
     CDS             complement(13097..13864)
                     /gene="tauB"
                     /locus_tag="ACHK8E_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP0344396.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13877..14839)
                     /gene="tauA"
                     /locus_tag="ACHK8E_002127"
     CDS             complement(13877..14839)
                     /gene="tauA"
                     /locus_tag="ACHK8E_002127"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0344397.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            15446..16117
                     /locus_tag="ACHK8E_002128"
     CDS             15446..16117
                     /locus_tag="ACHK8E_002128"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP0344398.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDIEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            16127..17323
                     /gene="yaiP"
                     /locus_tag="ACHK8E_002129"
     CDS             16127..17323
                     /gene="yaiP"
                     /locus_tag="ACHK8E_002129"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0344399.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            17358..17882
                     /locus_tag="ACHK8E_002130"
     CDS             17358..17882
                     /locus_tag="ACHK8E_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0344400.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            17884..18657
                     /gene="yaiO"
                     /locus_tag="ACHK8E_002131"
     CDS             17884..18657
                     /gene="yaiO"
                     /locus_tag="ACHK8E_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP0344401.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            18842..19117
                     /gene="frmR"
                     /locus_tag="ACHK8E_002132"
     CDS             18842..19117
                     /gene="frmR"
                     /locus_tag="ACHK8E_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP0344402.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            19152..20261
                     /gene="frmA"
                     /locus_tag="ACHK8E_002133"
     CDS             19152..20261
                     /gene="frmA"
                     /locus_tag="ACHK8E_002133"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP0344403.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            20353..21186
                     /gene="fghA"
                     /locus_tag="ACHK8E_002134"
     CDS             20353..21186
                     /gene="fghA"
                     /locus_tag="ACHK8E_002134"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0344404.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(21413..21952)
                     /gene="yaiL"
                     /locus_tag="ACHK8E_002135"
     CDS             complement(21413..21952)
                     /gene="yaiL"
                     /locus_tag="ACHK8E_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP0344405.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(22054..23265)
                     /gene="mhpT"
                     /locus_tag="ACHK8E_002136"
     CDS             complement(22054..23265)
                     /gene="mhpT"
                     /locus_tag="ACHK8E_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP0344406.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(23441..24454)
                     /gene="mhpE"
                     /locus_tag="ACHK8E_002137"
     CDS             complement(23441..24454)
                     /gene="mhpE"
                     /locus_tag="ACHK8E_002137"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP0344407.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(24451..25401)
                     /gene="mhpF"
                     /locus_tag="ACHK8E_002138"
     CDS             complement(24451..25401)
                     /gene="mhpF"
                     /locus_tag="ACHK8E_002138"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP0344408.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(25398..26207)
                     /gene="mhpD"
                     /locus_tag="ACHK8E_002139"
     CDS             complement(25398..26207)
                     /gene="mhpD"
                     /locus_tag="ACHK8E_002139"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP0344409.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(26217..27083)
                     /gene="mhpC"
                     /locus_tag="ACHK8E_002140"
     CDS             complement(26217..27083)
                     /gene="mhpC"
                     /locus_tag="ACHK8E_002140"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP0344410.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(27101..28045)
                     /gene="mhpB"
                     /locus_tag="ACHK8E_002141"
     CDS             complement(27101..28045)
                     /gene="mhpB"
                     /locus_tag="ACHK8E_002141"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP0344411.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(28047..29711)
                     /gene="mhpA"
                     /locus_tag="ACHK8E_002142"
     CDS             complement(28047..29711)
                     /gene="mhpA"
                     /locus_tag="ACHK8E_002142"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP0344412.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            29902..30735
                     /gene="mhpR"
                     /locus_tag="ACHK8E_002143"
     CDS             29902..30735
                     /gene="mhpR"
                     /locus_tag="ACHK8E_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP0344413.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            30812..31894
                     /gene="lacI"
                     /locus_tag="ACHK8E_002144"
     CDS             30812..31894
                     /gene="lacI"
                     /locus_tag="ACHK8E_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP0344414.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            32017..35091
                     /gene="lacZ"
                     /locus_tag="ACHK8E_002145"
     CDS             32017..35091
                     /gene="lacZ"
                     /locus_tag="ACHK8E_002145"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP0344415.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            35143..36396
                     /gene="lacY"
                     /locus_tag="ACHK8E_002146"
     CDS             35143..36396
                     /gene="lacY"
                     /locus_tag="ACHK8E_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP0344416.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            36462..37073
                     /gene="lacA"
                     /locus_tag="ACHK8E_002147"
     CDS             36462..37073
                     /gene="lacA"
                     /locus_tag="ACHK8E_002147"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP0344417.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(37176..38357)
                     /gene="cynX"
                     /locus_tag="ACHK8E_002148"
     CDS             complement(37176..38357)
                     /gene="cynX"
                     /locus_tag="ACHK8E_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP0344418.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(38363..38833)
                     /gene="cynS"
                     /locus_tag="ACHK8E_002149"
     CDS             complement(38363..38833)
                     /gene="cynS"
                     /locus_tag="ACHK8E_002149"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP0344419.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(38864..39523)
                     /gene="cynT"
                     /locus_tag="ACHK8E_002150"
     CDS             complement(38864..39523)
                     /gene="cynT"
                     /locus_tag="ACHK8E_002150"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP0344420.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            39633..40532
                     /gene="cynR"
                     /locus_tag="ACHK8E_002151"
     CDS             39633..40532
                     /gene="cynR"
                     /locus_tag="ACHK8E_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP0344421.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(40534..40625)
                     /locus_tag="ACHK8E_002152"
                     /pseudo
     CDS             complement(40534..40625)
                     /locus_tag="ACHK8E_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40665..41948)
                     /gene="codA"
                     /locus_tag="ACHK8E_002153"
     CDS             complement(40665..41948)
                     /gene="codA"
                     /locus_tag="ACHK8E_002153"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP0344422.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(41938..43197)
                     /gene="codB"
                     /locus_tag="ACHK8E_002154"
     CDS             complement(41938..43197)
                     /gene="codB"
                     /locus_tag="ACHK8E_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP0344423.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT        10599 a        11608 c        10959 g        10227 t
ORIGIN      
        1 gaaatatatt ctcttcgtta acttataatt aagagaaaaa aactcactgc atggccatac
       61 aagccaggaa taaattagct tcccgttcat catttttgtg atcttgcgca catcattgag
      121 ataacgcact ttcttcacca gtacgttgca cgctttgtca gcagaagatt gcaacattct
      181 gtcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt atgcactttg tgttagacgg
      241 gataggtttt taagatggaa aaactgcggg taggaatcgt ttttggtggt aaatcagcgg
      301 aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga tgccattgat aaaagtcgct
      361 tcgacgttgt gctgctgggc attgataaac aagggcaatg gcacgtcagc gatgccagca
      421 attatctgct aaatgcagac gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc
      481 ttgcgcaagt gccaggtaaa catgagcatc agcttatcga cgcgcaaaac ggtcagccgt
      541 tgccgacggt ggatgtcatt ttcccgattg tccacggtac gctgggcgaa gatggttcct
      601 tgcagggaat gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt ctggcttcag
      661 cagcctgtat ggacaaagat gtcaccaaac gtctgctgcg cgatgccggg ctgaacattg
      721 cgccatttat taccctgacg cgcgctaatc gtcacaacat cagttttgcc gaagtggagt
      781 ctaaactggg gttaccgctg tttgtaaaac cggctaatca gggctcttct gttggtgtca
      841 gcaaagtaac cagcgaagaa cagtacgcaa ttgccgtcga tctggcgttc gagttcgatc
      901 ataaagtgat cgttgagcaa gggataaaag gtcgtgagat cgaatgcgca gttctgggca
      961 acgacaatcc gcaagccagc acctgtggcg agatcgtact caccagcgat ttctatgcct
     1021 acgacaccaa gtacattgac gaagatggcg cgaaagtggt agttccggca gccattgcgc
     1081 cagaaatcaa cgataagatc cgggcgattg ccgttcaggc ttatcagacg ttgggatgcg
     1141 caggcatggc gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga
     1201 tcaacacact gcctggcttc accaacatca gtatgtatcc gaagctgtgg caagccagcg
     1261 gtctgggtta caccgacctg atcacgcgtt tgattgagct ggcgctggag cgtcacgctg
     1321 cggataacgc attgaaaacc acaatgtaat ttttaaatgc ctgataactt ttattgggca
     1381 tttaagactg atggcaaacg taagattgcc tgatgcgctt tgcttatcag gcctaccaga
     1441 taattgcaat atactgattt tgcatgattt tgtaggcagg gtaaggcgtt cgcgccgcat
     1501 ccggcaaaaa caacgagcac tttgtcagcc cccagaacgc ccaatcctct gttattgggc
     1561 gttttcagtt tcttcatcgc gacgacgaat aatcagcccc gccagccaga aactaatcac
     1621 ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc ccagtaaacc
     1681 caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt gccagtcacg
     1741 gcggattttt acaggcaagt tcatggcagc tccgttaacg aaaaacaaca tccttgcata
     1801 ttctgcaaca tccgtctgtg gcgtagacaa catttccgta attgttaata aatttaactt
     1861 aaggttgtca ttttgtgact cacgacgaag attcacgctt cgggaatatt cccaacatca
     1921 atttaccatg attctgatat tgacaatctt aatgatctgt cagacttgcc ataagccgct
     1981 ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg atttcaccct
     2041 gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg gcaacattgc
     2101 ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga acctgttagt
     2161 gcatgcgccc ggcgtctatg agcgcttaat gcaggtccag gaaacaggtc gcccacgggt
     2221 ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag ttggctgcct
     2281 catttttgca gtagtggcgc tatggctgca ctggcgacat cgccgccagt aagatttacg
     2341 ttgccagatg cggcctaaaa cgccttatct ggcatgacat tcctgtttga tcactgtttc
     2401 atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg
     2461 gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag tgatttaccc
     2521 gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc aaaagagaag
     2581 ctgtcccctt tctctaactg gcggcgaatt tcactgtcgc tcatccccag ttttcccgcc
     2641 agtttgcgcg ttgccagttc ggttttcccg ggctctttac tgtcgtaacg aattaaatca
     2701 ccgtccagtg ccagcttcac gccgctggcc ttactgaccg cgttctgaaa cgcggcatta
     2761 cggctggcgt accagccagc gttaaaatca gcaaaacggt atatcggcgc gctatagctg
     2821 gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcgacg actgaagact
     2881 tcctgacgga ctgtaccgtc cattttccac ggataccctt tggtatgctg ttcggcaaag
     2941 gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc aaacaacgtc
     3001 tgccccattg gcaccatgct gatcagatcg tcaaaaatcg cgctcaattg cttttcagta
     3061 cgcaccgaat ctaaccgttc gctatagctt ttgccgtttg gcgatttgat ttttagtgcg
     3121 gtatgcacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat ctcttgccag
     3181 gcgatcttgc ttaagcccgg aacagccgga tctgcctgat aatttgactc ctgctgcgcc
     3241 accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact ttcaaacgtg
     3301 gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat tttctgccgc
     3361 acgacgctcg ccacatctat cgctttttcg ccctttttca acggttgcgg agcctgacta
     3421 ctacatcccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg attgacgcgc
     3481 gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt gtcaccatcc
     3541 agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac cagtgtggtc
     3601 catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt agtaatctgc
     3661 gtcatcaggc cgagtgtaat cgtaccggca acaattgacg gaaacagcaa gaacaaaccg
     3721 aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat atagtgaaaa
     3781 tagaggcgga aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc
     3841 gcacgcgtgg catcgtcttc accataaacc agctctttac ggtaggcagc ctctactcgc
     3901 tggtttttaa actccagccc cggcagtttg atccctacca ctgccagtaa tccggtcccc
     3961 atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc gacaatcggc
     4021 agctccggca catgtgcgga gagcgttacc agcaccggca ggaaggcgat caacgtcatg
     4081 atagcgttga taaagctgac gcccatattc tccagcgttg aagcaaaacg catggtgtct
     4141 tcctgcacac gctgcgcggc cccttcgata tgacgcagtt gttgccagtt cgccatgtaa
     4201 tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa gttgttcagc
     4261 acactgatca ccaccgcgat cagcgcaatc cccagaaaga ctcccacttc gcggtaaaat
     4321 tgttcgatgg tgactttatg cggcgaactt agcgccgttt gaatcagatc atagaacggc
     4381 gcataccagg cgttgacggc aaccccgact tccaccaaaa accaggtgac gaagataatc
     4441 agcgcagtac cgagtattga ccagtattgc caacggtgcg ggctatagat aaaccagaac
     4501 aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa atccaacgac
     4561 cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg cgccacccaa
     4621 tcccccccac cggcttacca gaagataacg gcaatcaatg cccaaacaaa ggccgagaga
     4681 aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc taacttctta
     4741 tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca gccaaatgaa
     4801 accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgagcat aagaattgtt
     4861 tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc gcaggatcac
     4921 aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca
     4981 ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa tacgctcagt
     5041 taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc
     5101 tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa ccggagttcg
     5161 cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt gccacgggga
     5221 tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg cagttatggc gaaacgcgac
     5281 ctggtaataa cgttcgcccg cagctggatt cggtcatacg tattgcttcc ctcaccaagc
     5341 taatgaccag tgagatgctg gtgaaattgc tcgactaggg gaccgtgaag ttaaacgatc
     5401 cgctaagtaa atatgctccg ccaggcgcac gcgtaccgac ttacaatggg acgcccatta
     5461 cgctggtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc ggtggcgcgg
     5521 cacatcgtcc ggtatttgtc tggccaacgc gcgagcaacg ctggaaatac ctttctacgg
     5581 cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taacctcgca ttcgacctgc
     5641 tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt gaagagcaga
     5701 ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat cagtgccgcc
     5761 gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg gcaattggca
     5821 gcggcggcgt ctattccacg ccaggcgata tgatgcgctg gatgcagcag tatttgtcat
     5881 ctgattttta tcagcgcagt aaccaggctg atcgcatgca gacgttgatt tatcagcgcg
     5941 tgcagtttac aaaagtgatt ggcatggatg tccccggcaa agcggatgcg cttggtttag
     6001 gctgggtata tatggccccg aaagagggtc gtccagggat tattcagaag acaggtggtg
     6061 gtggcggttt cattacctat atggcgatga tcccgcagaa gaacattggc gcatttgtgg
     6121 tggtcactcg ttcgccattg acgcgcttca aaaatatgag cgatggcatc aatgacctgg
     6181 tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta atactctgaa
     6241 ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc gcctttacgc
     6301 aaagcagcga gaacttccgc aacgacagaa cgcgatatac gtgtacgttg atggatataa
     6361 ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga taataacgtc
     6421 gcgcggattt gttcgtagga attatgccca atgagctgaa cgtcgcgtaa ttccagaatt
     6481 ctattttgcc aggctaacca gtaaaaagcg tctcgccaga gttgattttg ttcaataaga
     6541 gtaatggttt gtttggctgg taaatgatat cccgcacagc ttccttctga tattaatttg
     6601 tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg agcctgggta
     6661 ataccgataa gtacgttttc ttctctacgc agagagataa cgccctctaa aataacgaaa
     6721 gtatcttcgc tattgacatc actggaaaat ataacttgct tttcattatt aaactcgaag
     6781 cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag aggtttaact
     6841 acagataaca tttgtgcgtc ctttgcagta atgcccgtca aatccttgac gggcattatt
     6901 tagattaaat tacctatttc ttcggagtga agaatattac caggtatatt taacacccac
     6961 attcgcggac cagtcttgat ctacgtcacc accaccgagg tagttagcat cggtataggc
     7021 gctgaagttc ttggtgaagc tgaactgagt acccagacca acacgtaccg cagacccttc
     7081 agtaccgtta tcgatggaat caccgttcac atcgttatcg ttgttagagt cgtcgtagac
     7141 gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg ctgtaggtga aggtataacc
     7201 tgcatctaca cccagttcat aacgcatgct gtcgtaagac tgaccatcaa ctttcatgtc
     7261 gttgctcagc tggtaatcat caccagactg gaacagacca gaaacgctgc cgtaaggagt
     7321 cacgtaacct gcatcaccca gtttgaagtc gtaaccggct ttcaaaccaa agccccaagc
     7381 gtcggagttg gtgctgccgt caacgtaagt accgttgctc atggttgcag acaggtcgtt
     7441 gttgaagtga gagtagctca agctaccgtc aacaaagacg ttgttcgcga agtgagcaga
     7501 agagtagatg taggcagtct ggctgtcttg atccacctga ccagaacggt cattcatatc
     7561 acctttagcg aagcctgcag ccgcaccgac gatccactta gcgttgttac cgtcaatttt
     7621 ggtatcaaca ccgaccatga tgccgttaac atcctgatca tagttgatgg tgccgttgtc
     7681 gccgttgaag ttaccaccga agtagcttac ccatgcgccg ccgttatcag ccagaccatg
     7741 acgagagttg gtcagacgag taccaacggt gtcttgttcc aggttccaga tattggtgtt
     7801 cgcagacggg atgctcagcg ccatgttagc gtagtcggtc agctccatct gttgcagaac
     7861 aacggtgtta ccacgctgct cagcctgata ggtgtatgca cccaggtcag ctttgttagc
     7921 agcagagaag gtcgcgttgc tgtttttgtc gttgacgtag atcagctcgt tgccgttgta
     7981 gtcagcgata gaaccagcac cagtagcgtt gtcgatacga actttgtagt taccggttgc
     8041 agctgcaaca tggtcaccgt tgttatcaac ttcgttctga ccagcttcga tagaagccgt
     8101 gttgtcgtta tcgccgttac cgttaatggt caggtgacca tcagagttca ttgcgattaa
     8161 gccataaccg tagtttgact tggtagtatc gttggtacgg tcgttggtca gatcggcatc
     8221 gagtacgaag cggttgcttt cgatgttgaa cacacctgcg tgaatgttac cactggtatc
     8281 agtagtgctg gtcaggttca gagtatcaac ctgcagagca ttcgccgcag tgttacctac
     8341 atccaggata ccgtcgttag tcactttgat gttgttagca tacagagcag catcacctac
     8401 agcccagcca gcagaaactt catcagaaat ggttacggta ctgttgtcga cagtcagtga
     8461 atcagttgca acatgaccat cttcgttgat gttcagcgca gaaagaccag tcagagagat
     8521 agagtcagaa atcagcgttg aatcagcaac attaacagtt gaaccactgt tgatgctcag
     8581 agtatcaatc agagaagttt tggtggtatc ccactgtgaa ccggtgttca gggtcacatt
     8641 gaacagaccg ctctggtaaa cttcgttacc gatgatgtga tcaaattcgc tgtattcaga
     8701 gtcgctgttt tctacaccaa cagttgatga aggccacagg ctgttagctg cgatgtcgat
     8761 cagagttgca gtggcttcag tattgattcc atatgtatag gagtcattta caccatcgtc
     8821 atcagtatca acaccaatct gatgcgcaga cattgccgca ccgacccact tgctgccatt
     8881 gttcagggtc agatccaggc gatcagtacc atcccaaccg ttagtatcta catcaccgtc
     8941 agcatcacga tagctatcac gaccttgtgg gaagaagttc tcatcaaaat tacttgagaa
     9001 gaatacatcg cccatcagtg tggaattgtc gaatgttgca gtggtttgca tggcgttatc
     9061 agcatatgga tgtgcaataa cagctaatgc aacatcatca gcattccacc catcaccatt
     9121 gtaatcacta gcattaccag tattaccaaa ccaaccagta gtaccttcat cagcccatga
     9181 accagaggtc acggtagaat ctttaacagt gatggtgtta ttaaatactt cgctgctgtt
     9241 tacaccagtg ctgacagtgt tgtcgtcgat atcttcccac tcataaccct gagtcagcgt
     9301 aatacctgct acatgagagt tattagagat ggacagatcg acttcctgat ccagagtgat
     9361 ggcagtacca atgttaaata catttacaac atgagtgtca gcggcattat tgtaagtgcc
     9421 gttatatgtg tagtgttcgt agttgtcatc gatcgttgaa ttatcaacgg tcagcgccag
     9481 acgatcatgg tagtagtcat ccgcacgatc agcacaatca gttgtcatgc actcggaata
     9541 aatcataccg tgaatagtgc tgtttttgat tgtcaggcta ttggcgttgg tattagtaga
     9601 aataccatcg tccagatagt aggtagaaat cacgccgtta acggtagcgt tattaatgac
     9661 cggatagata tcgccgttgt aataactatc agcggcatag ttattccagc ctacataacc
     9721 atcatagtaa acgtcatcag cataggtggc gtcgttgtag tgatggaaag tattgtaagt
     9781 ttgaccagaa atatcgttgg tcgctgcatt agcctgagag gtgatagcca gagtgcaagc
     9841 caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc atccctcctc
     9901 agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt atgtttcgcg
     9961 tttacgatta tgcggcgtta tagaggaatg gttcaattat tctttcccct aagtccgatt
    10021 gcggacatat ataaagcacg tgaacacaca caaagaaaga aataacaaaa acacaatatt
    10081 tcaatacgtt aacgaaagcg ttaattttaa aacatagacc atgaaaatat aaaacaacca
    10141 aaacagacaa ataatgtatc aataagcaat gaactttaag ttattcactt tttgaaaacg
    10201 tcaccaaatc cccaaaaaca ataatctatg gctataaata aaaattaaaa atattttcag
    10261 tgaatatgaa attattgcta tttctattac aaagatgtcc tgacctcttt ttcattctct
    10321 tcttgtcaac atcgcgacaa ctttcgtaaa acacacccag cctgcttcag gtatactatg
    10381 ctcctcgatt ccacaaacat caggcagacc atgacagatt taatccaacg ccctcgtcgc
    10441 ctgcgcaaat ctcctgcgct gcgcgctatg tttgaagaga caacacttag ccttaacgac
    10501 ctggtgttgc cgatctttgt tgaagaagaa attgacgact acaaagccgt tgaagccatg
    10561 ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg aaattgaacg catcgccaac
    10621 gccggtattc gttccgtgat gactttcggc atctctcacc ataccgatga aaccggcagc
    10681 gacgcctggc gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa gcagactgtg
    10741 ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat acacttctca cggtcactgc
    10801 ggtgtgctgt gcgagcatgg cgtcgacaac gacgcgactc tggaaaattt aggcaagcaa
    10861 gccgtggttg cagctgctgc aggcgcagat ttcatcgccc cttccgctgc gatggatggt
    10921 caggtacagg cgattcgcca ggcgctggac gctgcgggct ttaaagatac ggcgattatg
    10981 tcgtattcga ccaagttcgc ctcctccttt tatggcccgt tccgtgaagc tgccggaagc
    11041 gcattaaaag gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg tgaggcgatt
    11101 cgtgagtcac tgctggatga agcccagggc gcagactgcc tgatggttaa acctgccgga
    11161 gcatacctcg acatcgtgcg tgagctgcgt gaacgtactg aattgccgat tggcgcgtat
    11221 caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc tggcgggtgc tatagatgaa
    11281 gagaaagtcg tgctcgaaag cttaggttcg attaagcgtg cgggtgcgga tctgattttc
    11341 agctactttg cgctggattt ggctgagaag aagattctgc gttaatgatc attatgccgg
    11401 gttatccaac ccggcatttc ccccttaccc cgcccgataa aacggtttat ccccaagaat
    11461 cgtcgcccga tgcattatcc gccgctgtgg caggtaatcc gcattggcat agtgctgggt
    11521 tacgcggtta tcccaaatcg caatatcatt tggttgccag caccagcgca cctgaaactc
    11581 cggtttggtg atgtgggcaa ataaaaagcc cagcaaggct tcgctctctt tctcgctgac
    11641 atcaacaatt cgcgtggtaa agccttcatt cacaaacagc gcctgtttac cggtcaccgg
    11701 atgcgttcgc accaccggat gtagcaacgg tggatttttc gcgaccgcct cacgccagcg
    11761 ttgatgctca gcctcggttt tgcggtactt gtattccggg aacgatttac ggaaatcatg
    11821 ctccgcacgc aacccgctca acagctggcg gaagggaaca gagagcgcct catatgcggc
    11881 aataccactg gcccagagcg tatcgccgcc ggtcgagggt aactctttcg ccgccagaat
    11941 cgcccctgcg ggtggcgttt gaataaatgt cacatcggtg tgccagttgt cgttatccgg
    12001 cggattatcg ttatgggtat ccaggacgat gatctcgtca accccttcag catgagggta
    12061 aacagggtga atatgcagct cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgt
    12121 aatagcttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt aaagctgttc
    12181 aaactgatta tcgcttaacg ggcgcgttaa gtcggccccc gtaatttgtg cgccaatata
    12241 cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg ccagggcgtc
    12301 aggcggcgct gtaacgcgcg cagacccagt tctaaaagaa aggcgataat cgcaatcacc
    12361 gcgatccccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg aaccataaat
    12421 cctaaaccgc gcgttgcggc aatcagctcc gccgccacca gcgtagacca gcccacaccc
    12481 agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac
    12541 agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg
    12601 cttttcaccc ccgccagcgc cgacatcgcc accggcgcaa aaatcgctaa atagatcagt
    12661 aagatcttcg aggtttcgcc aataccaaac cagatcacca tcagcggcaa ataagccagc
    12721 ggcggcaccg gacgataaag ctcgattatc ggatccagaa tgccgcgcac cgtcgggctt
    12781 agccccatgg cgatcccgac cggaataccg atcaacaccg ccgcgagtag cgccagcaaa
    12841 atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa tccctgcggc
    12901 ccggcaatgg tgagcagttt tgccagtacc tgttgcggtg gcggtaaaaa cagcgggcta
    12961 atcagttgca gcgccgcgac tgcccaccat acagtgagca aaaccgctaa cgtaccaatg
    13021 ctgaaggtca cctgacgcga gagcggccag cgccatttca gccgatgcga atgcagtttt
    13081 tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg cttaaaacat
    13141 attcgcgcat ggcgatgaat tgtggatcgg acttgatgct gcggctcgac tctcccgcaa
    13201 caaagcggcg agcaaagttg agcggcagcc gctccagcac acggcctggg ccgggtgaaa
    13261 gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta atcaacagca
    13321 cctgctttcc cgtctcctgc cagagtttca gcagcagggt ttgcatctgg tcgcgggtga
    13381 aggcgtccag cgcaccaaac ggttcgtcga gtaataacag ctggggattt gccgccaacg
    13441 cgcgggcaat ccccacccgc tgacgttgac caccggaaag ctgccagatg tagcgttttt
    13501 ctgcgccttc cagccccact tttttcacca tctggtgtgc gatttccagt cgctgcattt
    13561 tctctacacc tgccagttgc aggccgaaca ccacgttgtc taccacatta cgccacggca
    13621 acagcccttc attctgaaaa accacgcccc gctctgctcc cggtccctca atgcacttac
    13681 ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga ttcagcaagg
    13741 tggttttgcc gcagccagac ggccccagaa ctaccagaag ctcgccgctt tccagcgtca
    13801 ggttgatatc ttccagtgcc ggtttgccgc cataatcggc gtaaagatga gaaatttgca
    13861 gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg taatcattcg
    13921 ccacggcggg gactttgccc tgctctttca aaaactgcgc ggtgtcgatg atcgctttgt
    13981 tcaccggtcc ggtcagttct gccgtttgct gctgcggcgt cagataggta ttccccttca
    14041 ccagccccgg aacgtcacct tcaggcacgc cgcttaaccg cgccagtttg ctgatgtttt
    14101 ccggctgttt cagccacgcg tctgggttag caatgtacgg ttgctgagca tcgatggcgc
    14161 ttttagcgaa cgctttcacg acctcaggat gtttctcggc aaaatctttc cgcacgaccc
    14221 agacgtccag cgtcggcgcg ccccactgcc cgacctgttc agaatcggtc agcaccttgc
    14281 cgtctttttc cagcgcgtta accgccggtg cccagacata agcaccatca atatctcccc
    14341 gctgccaggc ggcgataatt gcgggcggct gcaggttcac aatctccact tgcccaggtt
    14401 taatacccca gtgtttcagc gccgccagca ggctgtagtg ggtggtggag ataaacggta
    14461 cggcgatgcg cttgccaatc agatcttccg gtttgctgat agttttcttc accaccagcg
    14521 cttcggagtt acccagtttt gacgccagta agaagacttc aatcggcacc tgttggctgg
    14581 ctgccaccgc taatgggctg gaaccgaggt taccgatttg cacgtcgcca gaagccagcg
    14641 cccgcacgat gctggctccg ctgtcaaact tacgccagtc aacgctcgcc ccgctctctt
    14701 tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaagtt tgatacgcca
    14761 cagtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt
    14821 tacgcgatga aattgccatg attgtctgct cccctgtctt gttatgggag cagtattcag
    14881 gaataaaaac attcattaaa agaattagtt gttatcgcac agatgatttt attcttagcg
    14941 aaaaagtggc aaaggaaaga ttaaatatgc aattacaccc actctgacaa ttcagtaagt
    15001 tactgcactt cattttatta atgcataaac aagcagatta taatgtcttt tattttatta
    15061 acagcaatac atataatgaa accattatat tcacattgca tatataactg tatcattctc
    15121 aatcaaccac ataatacatt cacataatgt atataaccca tatttgtgca tagaacatat
    15181 ttttaatatg caattaattg acaaactaaa tcacatttaa atacaatata taaaacaaaa
    15241 taaccataca aaccaaacac acaaacactt aacatatgaa ctacataaag attatacagt
    15301 cttatccccg tacctggcag ccccttgttt ttcagctatc agctattttt aacacttaaa
    15361 aattagcatc tgtctgcgtc agaggttacg tctctgcggg cgtcgttgtg cttgtagtat
    15421 aataaataaa atggagtgtc ataaagtgga taaggtttta gattcagcga tcctttcttc
    15481 ggcaaataaa agaaagggta tccttgccat tggcgcgcat cctgacgata tagaattagg
    15541 ctgtggcgca tcgctagctc gccttgcgca aaaaggaatt tatattgcta ccgtggtgat
    15601 gactaccggt aattctggcg tagatggaat aatcgatcgc cacgaagaat cacgcaacgc
    15661 tctaaagata ttagggtgcc accaaactat tcatcttaat tttgctgaca cccgcgctca
    15721 tttacagctc aatgatatga tttctgccct ggaaaatatc ataaaaaatc aaattccctc
    15781 tgatattgaa attatacgtg tatataccat gcatgatgct gaccgccatc aggatcatct
    15841 tgcagtctat caagcctcaa tggttgcctg tcgcgctatt ccgcaaattc tcggctatga
    15901 aacaccaagt acctggcttt catttatgcc tcaggttttt gaatccgtga aagaagaata
    15961 tttttctctt aaactgactg cgttaaaaaa acataaaagt cagagccagc gagattatat
    16021 gcgcccggag cgtctgcgtg cagttgctca atttcgcggg caacaggtca atagcgatct
    16081 gggtgaaggt tttgttattc ataaaatgat tctttgagca aattgcatga aaacctggat
    16141 ctttatatgt atggccgtag caatattgct atggtttctg agtacgttaa ggcgtaagcc
    16201 cagccaaaag aaaggctgta ttgacgccat tatacctgct tataacgaag gcccgtgtct
    16261 ggcgcagtca ctggataatc tgctgcgtaa cccttatttc tgccgggtaa tttgcgttaa
    16321 cgacggctcc acggacaata ccgaagcggt catggcggaa gtcaaacgca aatggggcga
    16381 ccgctttatt gccgtcacgc aaaaaaatac cggtaaaggt ggtgcgctaa tgaatggcct
    16441 caattacgcc acctgcgatc aggttttttt aagtgatgcc gacacctatg ttccgcccga
    16501 tcaagacgga atgggctata tgctggcaga aatagagcgc ggtgctgatg ccgtaggcgg
    16561 cattccctct actgcgttga aaggcgcggg tctgttaccg cacatccgcg cgaccgtaaa
    16621 gttgccgatg attgttatga agcgcacgct acagcagctc ctgggcggcg caccgtttat
    16681 tatcagcggt gcctgcggga tgttccgtac tgatgtattg cgtaagttcg gtttctctga
    16741 tcgtactaaa gtcgaagacc ttgatctcac ctggacattg gtggcaaacg gctatcgtat
    16801 tcggcaggcg aatcgctgca tcgtataccc acaggaatgc aacagcccgc gtgaggaatg
    16861 gcgtcgctgg cggcgttgga ttgtgggcta cgcggtctgt atgcgcctgc ataaaagact
    16921 tttatttagc cgcttcggta tcttcagtat atttcctatg ctgttggttg tgctttatgg
    16981 cgttgggatt tatctcacta cctggtttaa tgaattcatc accaccgggc cacatggtgt
    17041 ggtgttggca atgttcccgc ttatctgggt cggcgtagtt tgtgttattg gtgcatttag
    17101 cgcctggttt catcgttgct ggttgttggt gcctttagca ccgctttccg ttgtgtatgt
    17161 attattagct tatgccatct ggattattta tggacttatt gcctttttta ctggacgcga
    17221 acctcagcgc gacaaaccca cccgctattc cgcactggtg gaagcgtcaa ccgcttattc
    17281 ccacccttct gtcacaggaa ctgaaaaact atctgaagct taatgttaaa gagaaaaata
    17341 ttcagattgc agaccaggtg attattgatg aaagtgccgg tgaggttgtt atcggcgcga
    17401 atacccgtat ttgccacggt gccgttattc agggtccggt agtgattggc gcaaactgcc
    17461 tgataggtaa ttatgcgttt attcgtcctg gcacaataat cagcaatggc gtaaaaattg
    17521 gttttgccac cgaaattaaa aatgcggtta ttgaagcgga agcaacgatt ggtccgcaat
    17581 gctttattgc cgactcggta gttgcaaacc aggcatattt gggcgcacaa gtacgaacca
    17641 gtaatcatcg tctggatgaa cagcccgtgt ctgttcgaac tccagaggga attatcgcta
    17701 ccggatgcga taaattaggt tgttatatcg ggcagcgttc acgccttggc gtacaagtta
    17761 ttattttgcc tgggcgaatt atttccccga acacgcaact tggcccgcgc gtgattgtag
    17821 aacgtaattt acctaccgga acttattcac tccgacaaga acttatccgt acaggagatt
    17881 aaaatgataa aacggacgtt attagcggcg gcaatattta gcacattgcc cgcttatgcc
    17941 gggttaactt ccattaccgc gggctacgat tttaccgatt attctggcga tcatggcaac
    18001 cgtaatttag cgtatgctga actggtggcg aaagttgaaa acgcaacgct gctttttaat
    18061 ctttcccagg ggcgtcgtga ttatgaaact gaacatttca atgccactcg cggtcagggt
    18121 gcggtctggt ataaatggaa taactggctg acaacccgaa cgggtattgc ctttgcggat
    18181 aatacgccgg tctttgcccg ccaggatttt cgtcaggata ttaacctggc cctgttgcca
    18241 aaaacgcttt tcacgaccgg ttatcgctac actaaatatt acgatgatgt cgaagtcgat
    18301 gcctggcaag gcggcgtatc cctctataca ggcccggtga tcaccagcta tcgctatacc
    18361 cattatgact ccagcgatgc aggtggtagt tacagcaata tgatttccgt gcgtctgaat
    18421 gacccgcgcg gcactggtta tacgcaacta tggctaagcc gcggaacagg tgcttacacc
    18481 tatgactgga cgccagaaac acgctacggc agcatgaaga gcgtcagttt gcaacgtatt
    18541 caaccgctga ctgagcaact taatctcggg ctgacggcag gtaaagtgtg gtacgacacc
    18601 ccaaccgatg attataacgg tctgcaactt gcagcccatc tgacctggaa attctgattc
    18661 cttctgccgc ccgctatccg gggcggcctt ccctgtcgat taggcccctc tttacttttt
    18721 gttatccgac tacattcacc ggataaattt tattctccag tgttatatac tatagagggg
    18781 tatgcattga catataacat acccccctat agtatattgc gtgcagatga tgaggtgcga
    18841 aatgcccagt actccggaag agaagaaaaa ggtccttact cgagttcgtc gtattcgggg
    18901 gcagattgat gctctggaac ggtcgctgga gggtgatgcc gaatgccgtg ccatactcca
    18961 acagatcgct gccgttcggg gcgcggctaa cggactgatg gcagaagtgc ttgaaagcca
    19021 tatccgggaa acgtttgacc gaaatgactg ctacagccgc gaagtcagcc aatccgttga
    19081 cgacactatt gaactggttc gtgcctatct taaatagctg aatctattac catattgagg
    19141 aagagcgaga gatgaaatca cgtgctgccg ttgcatttgc tcccggtaaa ccgctggaaa
    19201 tcgttgaaat tgacgttgca ccaccgaaaa aaggtgaagt gctgattaaa gtcacccata
    19261 ccggtgtttg ccataccgac gcatttaccc tctccggcga tgacccggaa ggtgtcttcc
    19321 cggtggttct cggtcacgaa ggggccgggg ttgtagttga agtcggtgaa ggcgtaacca
    19381 gcgtcaaacc tggcgaccat gtgatcccgc tttacaccgc agagtgcggc gagtgtgagt
    19441 tctgtcgttc tggcaaaacc aacctgtgtg tcgcggttcg cgaaacgcag ggcaaaggct
    19501 tgatgccaga cggcaccacc cgtttttctt acaacgggca gccgctttat cactacatgg
    19561 gatgctcaac attcagtgaa tacaccgtgg tcgcggaagt gtctctggcc aaaattaatc
    19621 cagaagcaaa ccatgaacac gtctgcctgc tgggctgtgg cgtgaccacc ggtattggcg
    19681 cggtacacaa cacagctaaa gtccagccag gtgattctgt tgccgtgttt ggtcttggcg
    19741 cgattggtct ggcagtggtt cagggcgcgc gtcaggcgaa agcgggacgg attatcgcta
    19801 tcgataccaa cccgaagaaa ttcgatctgg cgcgtcgctt cggtgccacc gactgcatta
    19861 acccgaatga ctacgacaaa ccgattaaag atgtcctgct ggatatcaac aaatggggta
    19921 tcgaccatac ctttgaatgc atcggtaacg tcaacgtgat gcgtgcggcg ctggaaagtg
    19981 cgcaccgcgg ctggggtcag tcggtgatca tcggggtagc tggttctggt caggaaatct
    20041 ccacccgtcc attccagttg gtcaccggtc gcgtatggaa aggttccgcg tttggcggcg
    20101 tgaaaggtcg ttcccagtta ccgggtatgg ttgaagatgc gatgaaaggc gatatcgatc
    20161 tggaaccgtt tgtcacgcat accatgagcc tggatgaaat taatgacgcc tttgacctga
    20221 tgcatgaagg caaatccatt cgaaccgtaa ttcgttactg atttcccgca ggtttacccg
    20281 tccagttcag acggggttct taatactctc cccgggcagc cgtccggggg attaaccgtg
    20341 agataatgac tgatggaact cattgaaaaa catgccagct ttggcggctg gcaaaatgtg
    20401 tatcggcatt attccccatc actgaaatgt gaaatgaatg tcggcgtcta tctcccgcca
    20461 aaagccgcaa atgaaaaatt gccggtgttg tactggcttt caggcctgac ctgcaacgag
    20521 cagaatttca ttactaaatc ggggatgcag cgttacgcgg ctgagcacaa cattattgtt
    20581 gttgcgccag acaccagtcc gcgaggcagt catgtcgcag atgccgaccg ttacgatctc
    20641 gggcaaggtg ccgggtttta cctgaacgca acgcaagcgc cgtggaatga acattacaaa
    20701 atgtatgact atatccgcaa cgagctgccg gatttagtga tgcaacattt tccggcaacg
    20761 accagaaagt ctatctcagg tcattctatg ggcgggctgg gcgcgctggt gctggcgtta
    20821 cgtaacccag atgaatatgt cagcgtctcg gcgtttgcgc ccattgtctc cccatcgcaa
    20881 gtaccgtggg gacagcaagc ctttaccgca tatcttggtg aaaataaaga tgcctggttg
    20941 gattacgatc cggtgagtct tatttcacaa ggtcaacgcg ttgcggaaat aatggttgat
    21001 caggggttga gtgatgattt ttacgcagaa cagctgcgga ctccaaatct tgaaaagatc
    21061 tgccaggaga tgaatatcaa aacgttaatc cgttatcacg agggttatga tcacagctat
    21121 tattttgtct ccagttttat tggcgagcat attgcgtacc acgccaataa actgaatatg
    21181 cgttgagcat ccgcgcacgc atgccggatg cggcgtaaac gccttatccg gcctacattt
    21241 cacccgtaag ccgtaggcct gataagacgc gaaaagcgtc gcatcaggca tctgcgcacg
    21301 gctgccggat gcggcgtgaa cgccttatcc ggcctacact tcgcccgtaa accgtaggcc
    21361 tgataagacg cgtaagcgtc gcatcaggca tcactgcacg gcttatctat gtttaccaca
    21421 tcaaataatc aggcactttg aagtcggcat acggatcgtc ttcatcctgc tcttcggcgc
    21481 tgagcgcgct gtgcaacaca atactgctgg catcacgctg agcaatttta tcggcaacac
    21541 tggcgggaat aatggcgtat tcaccttcgc tattgttatc gaccaacaag cgggcaatcg
    21601 ccagacgacc attaatcaac tgcgcctgtg tgagcttatc gacaaaaatc ttcttaatca
    21661 gattgccgtc agtgaagtta aaaccaatat cgccattggc aatggtgatt cggttcattt
    21721 caatgagctg cttcacctgg gctttatatt ctttcgccaa cgccgcttgt ttttgctgtt
    21781 cgctaagctg tttatcacgc tcaagctgtg cctttttatt ttcttctacc gccgcccgag
    21841 cttcacgcgc ctgaacgcgc gattttttcg ccgttctctc gaccttcgcc gcttttttgc
    21901 tggtgactaa tcctgctttg agcaactgct cttgtaaggt aagttttgcc atctttgttt
    21961 ccaaacccgc tgaataattt ttagcgagta tacctgtaaa cgccgtaggt gtattggggg
    22021 caagtccctt tcgcccctgc ttaataaggc gcgtcaggca tcggcgcacg gctgcattcg
    22081 tgatctccgg ctcatcaaaa taaatactgc caggccagca acaagaatac ccggcgcaga
    22141 agcggccatt acgccaaccg tgccaatgcc taatgccagc attttcccgg ccagtaacgg
    22201 accgctcata gcccccagac gccctaccgc cacggctgtt cccacacctg ttgcgcggat
    22261 ctgcgaactg taaaacaacg gtgccagggc atacaaaacg ctttgcccac ctgtcgcaaa
    22321 caaccccgcg acgaatcccg ccagcaacat accgttaaat gacgacaccg ttcccagcgc
    22381 cagcagcgaa gctaacatgc cgctataaat cagtagcgac atggttactg gacgcagctt
    22441 atccatcaat gcgcccaaca ttaacgtccc gcttgccgcc cccatttgca gtgcaaacat
    22501 cacccctgcc gcctgcgatg gctggaatcc ttgctccacc aaaagcagcg gtagccagtt
    22561 gatcaacatg tagaccacca gcagagtgaa gaaataacac aaccacagca gcagcgtcgc
    22621 ggttgccgtt tctggcgcaa ataacgcacg cagtggtggc gcagcctgtt tttcgccagc
    22681 gaacaccgcc gactccggca gccagcgcat taatagcggc accagaatca acggcaccac
    22741 accacctacc caaaacaccg tttgccatgc taagtttgcc cccgcgaaac ccagtgtcgc
    22801 cgccagcgcc gcgccaatgg ggacaccaca atacatcagg ctcactgccg tcccacgaaa
    22861 acgtggaccc gcggcttcag acgtcagggc gatcagattc ggcaacgccg cccccagccc
    22921 gacaccggtc atcagccgcg caaagaccag tgaggggaaa tcccaggcaa tcgccgttgc
    22981 cagtgagaac aaaccaaaca gcgcaactga gccaatcaaa atgcgcttgc gaccataacg
    23041 gtccgccagc attccgccaa ccaacgcgcc gggtagcaaa ccgagtattc cggcgctgaa
    23101 tatccagccc atttgcattt tatcgagtgc gaaagcctgg gcgatgccac ccgccgcaat
    23161 gccagccgcc tgaagatcca gtccttccat cagagcgacc aaaaaacaaa gcccgatggt
    23221 cagcatcagg cgggttgaag atgatgaagg ggtacgagtc gacataagat gaacctcgtt
    23281 gcgagtgcag gtgaatcgcg gctgatacat tgtcagccgc gtaatcagaa ccatttaatg
    23341 actgcctacg ggcgaaatat aggccggata aggcgtttac gccgcatccg gcagtcgtgc
    23401 tgggatgggc atacaaaatg cacgttctcg gcagtcatca ttatttgttg ttgcgcagat
    23461 ccagcgccac gtcgacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct
    23521 caacgagaat atccaccgcg cttaagccat aacgcgccgc cgccgtttca cagtgacgca
    23581 ggaagctcga gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg
    23641 gtcgatcctg caacggacgc accaggtcgt cagcggcatc cattaacgca tagagatcgg
    23701 tcccatgctg ccaacccagt ttatccgcgg cggcaataaa aacttccagc ggcgcgttac
    23761 ctgcgcccgc gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg
    23821 ccgcgataga gttcgccacg ccaagactca ggttatggtg agcgtgtatg ccggtttgcg
    23881 tttctggttt cagcactgct ttcagagcgc ggaaacggtc acggatatcg ctcatgttca
    23941 tcgcaccgcc agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg
    24001 cctgctttgc gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt
    24061 ccattccgag ctcgcgggca tactgaatat gctgggcgga aacatcagct tcggtacagt
    24121 gcgtagccac acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag
    24181 tgccgattcc aggcagcaac aacgtcgcga ttttggcgtg cttcaccaca tccgccgccg
    24241 cttcaatcca ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccttgca
    24301 aaccgtcgcc gtgggccact tcaatcgaat ccacgcgggc atcgtccagt gctttggcaa
    24361 cctggcgaac gttttccaat gaatactgat gacgaatggc gtgcataccg tcacgcaacg
    24421 tgacgtccga gatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag
    24481 tgactgggcc attttttccg ctgtcgccag cgcactggaa gtcataatgt cgaggttgcc
    24541 tgcataggca ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ccgttttcag
    24601 tccggagaac tgccccacgc ccggcaaatt aaccggttta tcctgcggga taacttcaaa
    24661 ctgcacgcgc tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc
    24721 attgattgag gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc
    24781 acgcatcatc aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc
    24841 gccacccacc acttcaatgg cgcgcgaagt ggtttccgta aattcatcga tattggcacg
    24901 cgtgccaggt ccggcagatt tactggcaat agaagcgata atttcggcgt aatgaacacg
    24961 cgccacgcgt gaaactgccg ccaccattgg aatggtggcc tggccgccgc aggtgaccat
    25021 gttgacgttc agttgagcga cgttctcctc gaggttaacg accggcacgc agtaggggcc
    25081 gatggcagca ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gcaaagcggc
    25141 atcgtttttc acatgcgcac ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa
    25201 ttcaggcatg ttcatcaggc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct
    25261 ggcacgcgcc agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg
    25321 ctggccgtga cgcaaaattt taatcatcag atcggtgcca atgttgcctg aaccgataat
    25381 ggcgacttta cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca
    25441 gcaactgaac ctatgccttc aatatgggct tcaaaacgat cgcccgcatt caccgccacc
    25501 atcggaccta atgccccggt aagaatgata tctccggcgc gcagcggttc acccaggctg
    25561 gccattttgc gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg
    25621 cgcccgctgg aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct
    25681 aaccccgccg gacgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct
    25741 gccacggtat cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacttccagc
    25801 gccggaagta cccattcaat ggcgttatac aattcgtcga aggtgatatc ggttgcgggt
    25861 agatcgcggt tcaacaccag tgcaatctcc gcttcaatgc ggggttgcag aacacgggaa
    25921 aaaggaatgg tttcgttatc gccataacac atgtcggcaa ataatgtccc gaaatccggt
    25981 tgatcaacgc ccagttgttg ctgcactttc ggatgcgtca gccccacttt gcgccccacc
    26041 actcgccgtc cctgagcgac gtcgtgttgc acattgatgt gctgaatggc gtaggccgct
    26101 tcagcgttat cgataccaat cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg
    26161 gcgcgacgta aatccgccgc cagttgctca agagtatgct tcgtcatgac cgttccttaa
    26221 gggcgtgcga ggaaattcag caccagttga ttgaaagcgt cggcatgttc ccactgcgcc
    26281 cagtgaccac agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc
    26341 agacccgcat ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct
    26401 ttgatttccg ccagacgtgg gccaaaatcc gggaactgtt tcggattggc ttccaggctc
    26461 ttaacgaagt tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac
    26521 agggcgtcgg tcaaatcgct ggtatcaaaa acgaagatat ccatcatcag cttcaggttt
    26581 tcgatagtcg gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc
    26641 gtaaacaaac tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc
    26701 ggccagttaa gggtgaacgc cacagaactg tggccgccca tcgagttgcc cagcaggtgg
    26761 attttggcga tatccagttg atccaccacg cttttcagga ttcgcgcatt aagatccgat
    26821 cgtgaaccac tattaacgat cgaatcgctc ttaccccaac ccggacaatc cagcaggatc
    26881 acccgatagc ccgcctttac cagcggatcg atattgcggc tgaagttcgc ccagccagta
    26941 gcacccgggc cggaaccatg cagcaggaca acagtttcgt cgccttgtcc gcagtcatta
    27001 aaatggatgc gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct
    27061 tcggtttgtg gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc
    27121 ttaacgagcc aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac
    27181 gccagttgcc aaacgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg
    27241 tcgactttcc ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc
    27301 gtccctgctc cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagcg
    27361 ttctctgatc ctcaacaaac ttctcagcgg cgctaatcac ccgttgctga cgcaattcgc
    27421 gctcactggc gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg
    27481 ctttcgccag ttctggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca
    27541 cgcgtttatt gagagtactg gtgaaacgtc caatggcttc ccccaacatg cgggtacgct
    27601 ggaaaccggg caacggcgtg gcgacaccgt tgatgaacac aggcagaacc ggcaccttat
    27661 ccagcccacc gagcaggaac tccagcggct gggcgaaccc gtggtccacc tgcatacagt
    27721 aagaaacggc aagatcgatt ccgctcttca tgacggcatg cgcacaggcc tccgccagct
    27781 caacaggcac gggcagctct cctgccgcac taccgaaatc accaattgcc gtcgctccaa
    27841 cgcctaaaca gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa
    27901 acagcaccac caactcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc
    27961 cattgacctc atcgagcacc tcttgcgccg ggtcgacata tcccaccagc ggcgagtggg
    28021 aaagacagtg aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca
    28081 gggcgggtca gcgtcattac cgacgccagt ttattcagcg tattacccag agtttgcgga
    28141 atggcggtgg cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgc
    28201 gcgaaccagc tacgcaggcg accttgcgta tcgcccacgc gtagtacgcc gtcgtggtta
    28261 tcctgtgcgg tatgaatttg cacttccggc accacctgaa tgaagcgtgt gcccaacgcg
    28321 cgccactgct ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt
    28381 accgcgaagt tcgcgccgat cgcgttatcg agcagcgtca cgtcgccgtt ttccagcgtg
    28441 actttcggct gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc
    28501 agcgcaccgc cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt
    28561 actggcggca gataattcaa cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt
    28621 ttcggcggag ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga
    28681 tcgcgacgtt cttgttgata ggtatcgagc agcgcatcgc gggctttccc ctggataacc
    28741 aacgccagtt tccatgcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc
    28801 tgccacaccg gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacgaaaa
    28861 cgttgcgcca gtcgcgcgtt gtgggtgtag acacgctggc gaatcaattc aacattgtcc
    28921 ggattaggca gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgttct
    28981 tcggtttctc ccggcatcac cataaattca aagcgacgta ccgcatgagg cagcgcggca
    29041 gaaacatacg ggcgcaccgg atcacaacac aaatagatat gcggcgtact taacggatcg
    29101 ttggcgatat ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc
    29161 agggtgcgac ggacaaaact tgccccacca tcacaggcca ccagccattg ggctttgacc
    29221 gtttcccgct gcccttctgc cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg
    29281 aaggcctcca gctcgcggga aaacaggcag cgcacattcg gaaaacgcga caatccttcc
    29341 agcatcaccg catcgacctg aggctgaata aaggcgttac ggcgcggcca gccaaattca
    29401 tcggtcattg gctgaatatc agcaaaacag cgaccttttg gggtgagaaa acgcatcgcg
    29461 tgccacggcg tagtgtgcgg cagaacatta tcgaccaggc cgaccgactg catggtgcgc
    29521 agcgcctcgt catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc
    29581 accaccagca cgtcaatgcc catctggccg agatagttcg ccatcatcag cccgaccgga
    29641 ccggcaccag cgatcgccac ctgaacgcta tggttaacag caggctggat gtcaggatgt
    29701 tgtattgcca tttcagtacc tcacgactcg gacaaaatgc cgttgcgcgc acagtacggc
    29761 gcaacttatt ttgttaaaaa catgtaaatg attttttatt gtgcgctcag tataggaagg
    29821 gtgttttcgg ctacaatcaa aacatgcccg aatgtgcacc aagtgcacaa cgttgtttta
    29881 actatagaaa tgtcaattaa tatgcagaac aatgagcaga cggcatacaa aaccgtgcgc
    29941 ggcttaaccc gcggtctaat gttattaaat atgttaaata aacttgatgg cggtgccagc
    30001 gtcgggctgc tggcggaact cagcggcctg catcgcacca ctgtgcggcg actgctggag
    30061 acgctgcagg aagagggata tgtccggcgt agtccctccg atgatagttt ccgtttgacc
    30121 atcaaagtgc ggcaattaag cgaaggattt cgtgacgaac agtggatttc tgcactggcg
    30181 gccccactgc tgggcgatct gttgcgcgaa gtggtatggc cgacagatgt gtccaccctg
    30241 gatgttgatg caatggtggt acgcgaaact acccatcgtt tcagccgctt atcttttcac
    30301 cgggcaatgg tcgggcgacg gttaccgctt ctgaaaaccg cctcgggcct gacctggctg
    30361 gccttttgcc cggaacaaga acgcaaggaa ttaatcgaga tgttagcctc ccgccccggt
    30421 gatgactatc aactggcacg ggaaccgtta aagctggaag ccattctggc gcgcgcgcgc
    30481 aaagaggggt acggacagaa ctaccgcggc tgggatcaag aagaaaagat cgcctctatt
    30541 gccgtaccgc tgcgcagtga acaacgggtg attggctgtc tgaatctggt gtatatggcg
    30601 agcgcaatga ccattgaaca ggcagcggaa aagcatcttc cggcgctaca acgggtggca
    30661 aaacagatcg aagaaggggt tgaatcgcag gctattctgg tggccggacg gcgaagcggc
    30721 gtgcatttac gttgacacca tcgaatggcg caaaaccttt cgtggtatgg catgatagcg
    30781 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgctat acgatgtcgc
    30841 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg cctgccacgt
    30901 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
    30961 ccgcgtggca caacaactgg cgggcaagca gtcgttgctg attggcgttg ccacctccag
    31021 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
    31081 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
    31141 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
    31201 ccaggatgcc attgctgtgg aagctgcctg cgctaatgtt ccggctttat ttcttgatgt
    31261 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
    31321 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcag gtccattaag
    31381 ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa tatctcacac gcaatcaaat
    31441 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
    31501 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
    31561 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
    31621 agtgggatac gacgataccg aagacagctc gtgttatatc ccgccgttaa ccaccatcaa
    31681 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
    31741 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
    31801 tccgcccaat acgcaaaccg cttctcccca ggcgttggca gattctttaa tgcagctggc
    31861 gcgacaaatt tcccgactcg aaagcgggca gtgagcgcaa cgcaattaat gtgagtcagc
    31921 tcactcatta ggcaccccag gctttacact ttatgcgtcc ggctcgtatg ttgtgtggaa
    31981 ttgtgagcgg ataacaattt cacacaggat acagctatga ctatgattac ggattcactg
    32041 gccgtcgtat tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    32101 gcggcacatc cccctttcgc cagctggcgt aatagcaaag aggcccgcac cgatcgccct
    32161 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
    32221 gcggtgccgg aaagctggct ggagtgcgat cttcctgacg ccgatactgt cgtcgtcccc
    32281 tcaaactggc agatgcacgg ttacgacgcg cccatctaca ccaacgtgac ctatcccatt
    32341 acggtcaatc cgccgtttgt tcccgcggag aatccgacag gttgttactc gctcacattt
    32401 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaat
    32461 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagccgtttg
    32521 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
    32581 gtactgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg aatgagcggc
    32641 attttccgtg acatctcgtt gctgcacaaa ccgaccacac aaatcagcga tttccatgtt
    32701 accactcgct ttaatgatga tttcagccgc gcggtactgg aggcagaagt tcaggtgtac
    32761 ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt ggcagggtga aacgcaggtc
    32821 gccagcggca ccacgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
    32881 cgcgtcacgc tacgtctgaa cgtcgaaaac ccggcgctgt ggagcgccga aatcccgaat
    32941 atctatcgtg cggtggttga actgcacact gccgacggca cgctgattga agcagaagcc
    33001 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
    33061 ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
    33121 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
    33181 cgctgttcgc attatccgaa ccatccgctg tggtacaccc tgtgcgaccg ctacggcctg
    33241 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
    33301 gatgatccgc gctggttacc ggccatgagc gaacgcgtaa cgcgaatggt gctgcgcgat
    33361 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcgggcca cggcgctaat
    33421 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
    33481 ggcggcggag ccgacacctc cgcaaccgat attatttgcc cgatgtacgc gcgcgtggat
    33541 gaagaccagc cctttccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctgcct
    33601 ggagaactgc gcccgctgat cctttgcgaa tatgcccacg cgatgggtaa cagtcttggc
    33661 ggcttcgcta aatactggca ggcatttcgt cagtaccccc gtttacaggg cggcttcgtc
    33721 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcagct
    33781 tacggcggtg attttggcga tacgccaaac gatcgccagt tctgcatgaa cggtctggtc
    33841 tttgccgacc gcacgccgca tccggcgctg acggaagcaa aacaccagca gcagtttttc
    33901 cagttccgtt tatccgggcg aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
    33961 gataacgagc tcctgcactg gtcggtggcg ctggatggca agccgctggc aagcggtgaa
    34021 atgcctctgg atgttgctcc acaagataaa cagttgattg aactgcctga actaccgcag
    34081 ccggagagcg ccggacaact ctggcttacg gtacacgtag tgcaaccgaa cgcgaccgca
    34141 tggtcagaag ccggacacat cagcgcctgg cagcaatggc gtctgacgga aaacctcagc
    34201 gtgacactcc cctccgcgcc ccacgccatc ccgcaactga ccaccagcga aacggatttt
    34261 tgcatcgagc tgggtaataa acgttggcaa tttaaccgcc aatcaggctt tctttcacag
    34321 atgtggattg gcgatgaaaa acaactgctg acgccgctgc gcgatcagtt cacccgcgca
    34381 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggta
    34441 gaacgctgga aggcggcggg ccattaccag accgaagtgg cgttgttgca gtgcacggca
    34501 gatacacttg ccgacgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
    34561 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
    34621 gttgatgttg aagtagcgag cgatacaccg catccggcac ggattggcct gacctgccag
    34681 ctggcgcagg tcgcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
    34741 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
    34801 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
    34861 ccacaccagt ggcgcggcga cttccagttc aatatcagtc gctacagcca acaacaactg
    34921 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
    34981 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtgtc ggcggaattc
    35041 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
    35101 ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac
    35161 acaaactttt ggatgttcgg tttattcttt ttcttttact tttttatcat gggggcctac
    35221 ttcccgtttt tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacaggt
    35281 attatttttg ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt
    35341 tctgacaaac tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg
    35401 tttgcgccgt tctttatttt tatcttcggg ccactattac aatacaacat tttagtagga
    35461 tcgattgttg gtggtattta tcttggcttt tgttttaacg ccggtgcgcc agcagtagag
    35521 gcatttatcg agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt
    35581 ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat
    35641 cagtttgttt tctggctggg ttctggctgt gcactcatcc tcgccgtttt gctctttttc
    35701 gccaaaacgg atgcgccctc ttccgccacg gttgccaatg cggtaggtgc caaccattcg
    35761 gcatttagcc ttaagctggc gctggaactg ttcagacagc caaaactgtg gtttttgtca
    35821 ctgtatgtta tcggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc
    35881 tttacttcgt tctttgccac aggtgaacag ggtacgcggg tatttggcta cgtaacgaca
    35941 atgggcgaat tacttaacgc ctcgattatg ttcttcgcgc cgctgatcat taatcgcatc
    36001 ggtgggaaaa acgccctgct gctggctggc actattatgt ctgtacgtat tattggctca
    36061 tcgttcgcca cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta
    36121 ccgttcctgc tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca
    36181 gcgacgattt atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct
    36241 gtactggcgg gcaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt
    36301 ctggtggcgt tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggtccgctt
    36361 tctctactgc gtcgtcaggt gaatgaagtc gcttaagcaa ttaatgtcgg atgcggcgcg
    36421 agcgccttat ccgaccaaca tatcaggacg gaatgatcgc aatgaacatg ccaatgaccg
    36481 aaagaataaa agcaggcaag ctatttaccg atatgtgcga aggcttaccg gaaaaaagac
    36541 ttcgtgggaa aacgttaatg tatgagttta atcactcgca tccatcagaa gttgaaaaaa
    36601 gagaaagcct gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaaccac
    36661 ccgtccattt ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca
    36721 atttaaccat tgtagacgac tacacggtaa cgatcggtga taacgtattg attgcaccca
    36781 atgttactct ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggtgaga
    36841 tgtactcttt tccgataacg attggcaata acgtctggat cggaagtcat gtggtcatta
    36901 atccaggcgt caccatcggg gataattctg ttattggcgc gggtagtgtc gtcataaaag
    36961 acattccacc aaacgtcgtg gctgctggcg ttccttgccg ggttattcgc gaaataaacg
    37021 accgggataa gcactattat tacaaagatt ataaagttga atcgtcagtt taaattataa
    37081 aaattgcctg atgcgctccg cttatcaggc ctacaagttc agtgatctac gtaggccgca
    37141 tccaacgtaa acaaagcgca ggaacaagcg tcgcatcatg cctctttgac ccacagctgc
    37201 ggaaaacgtg ctggtgcaaa acgcagggtt atgatcatca acccaacgac gcacagcgca
    37261 tgaaacgccc agtccatcaa gtaattgcca ctgatactac gcagcacacc agaaaaccac
    37321 ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa acgccaccag tttgccagca
    37381 atagcgggtt gcgcagagtg atcgagtgcc agcagcaaac agagcggaaa tgcgccgccc
    37441 agacctaaac cacacaccat cgcccagaag accggcaatt gcagcggcag ccagataaag
    37501 ccgcagaaac ccaccagttg taacgccagc gccagcatca aaagtttgcg ccgatcctga
    37561 tggcgagcca tagcaggcat cagcaatgcc cccgcagctt gcccaagcgt catcaacgcc
    37621 agcaaggaac cgctgtactg cgcgctggca ccaatctcaa tatagaaagc gggtaaccag
    37681 gcaatcaggc tggcgtaacc accgttaatc agaccgaagt aaacacccag cgtccacgcg
    37741 cggggagtaa ataccacgcg aaccggagtg gttgttgtct tgtgagaaga ggcgacctcg
    37801 cgggcgtttt gccaccacca ggcaaagagc gcaacaacgg caggcagcgc ccaccaggcg
    37861 agtgtttgat accaggtttc gctatgttga actaaccagg gcgtaatggc ggcaccaagt
    37921 ccaccgccgc ccattagagc cgcggaccac agccccatca ccagtggcgt acggtgctga
    37981 aaccgccgct taattaccga aggcatcact gcctgaatga tgccgatccc cactccacca
    38041 agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa gctcacgcat caatgcaccg
    38101 acggcaatca gtaacaaact gatggcgacg ctgcgacgtt cgctgacatg ctgatgaagc
    38161 cagcttccgg ccagcgccag cccgcccatg gtaaccaccg gcagagcggt caacagggca
    38221 gccacgctaa agctcattcc gcttgcctgg cgcaattgcg gtagcagtgg cccgacggag
    38281 gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa ccagcagcat cgtgaacgtc
    38341 ttttgatggt tacgcatggc tgtcagaacg gtttggtcgg cagatattta ccatctaagg
    38401 tgatgaccgc acgttcgcca ccctccgggt ccgccacttt cttaacgtcg agtttgaagt
    38461 taatcgcgct gataatgcca tcgccaaact tctcatgaac caacgctttc agggttgtgc
    38521 cgtacacctg caacatttca tagaaacgat acatcgttgg gtcggttgga atacggtcat
    38581 caatgcagcc acgcagtgga atcatctgca acagtagaat ggcgtcttcg tcgagatcca
    38641 gcttcgctcc gaccaggcgg gcggcgtcgg caggaagcgc ctgctgcccc agcaaagccg
    38701 cggttacaaa ggcttctgcc agaccggtgc cgtcggcaat ctcggcaaat gagagatctt
    38761 ttttagcttt gctgagcaaa atggcatcgg caagatcaag acgaatattg cggttaattt
    38821 gcgactgaat catggtggat ctcctgatgg tttaaaaata aggttacgct gcggtcggtt
    38881 ggcgtagcgg tatggcacaa acgcgaggat tagcggccag tggcacaaac tggcgggttg
    38941 cgccgtcaaa agctgcgatg ctgccgcttt caatgtcgta gacccagccg tgcagggcaa
    39001 tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt ttgcaaatta gccaactgag
    39061 caatgacgtt ttcacgtacc atcgccgctg cttttgacgg taaatcggga tgcgggcgcg
    39121 cctcattaac gacgcgggct gaatcggcat aacgcagcca gtgggagacg gcaggcatat
    39181 ggtccatgca ctgacagctg gcaatggagg tcatcgcgcc acagttggaa tgaccgcaaa
    39241 tcacaatgtc agatacccga agtgcagcga cggcatactc caccgaagca gaaacgccac
    39301 cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt gcgaataacg aacagatcgc
    39361 caggctcacg ttgcgtcacc agctcaggga ccagacggct gtcggagcag gagataaaaa
    39421 gggtgcgcgg gctttgctgt gtcgccagct gtttaaacaa ggcttcccgc ttcggaaatg
    39481 cctcgcgctg gaatttaagg aatccatcaa taatctcttt caccgttaac ctctgtctgt
    39541 ctctggaatg agaggcagac tactcgccga ttgtcataag gtaaaagact catttatgat
    39601 gagttctatt ggatttactt ataggttggc gaatgctctc tcgacatatc aattactttc
    39661 ttgccgtggc tgaacatggc agcttcaccc gtgcagccag tgcgttgcac gtctcccagc
    39721 ctgcgctttc ccagcagatt cgccagttag aggaaagttt gggcgtgccg ctgtttgacc
    39781 gtagcgggcg aacgattcgt ctcactgatg caggagaagt ctggcgacag tacgccagcc
    39841 gggcgttaca ggaactgggg gcgggtaaac gggcgattca tgatgttgcc gatctgacgc
    39901 gaggatcgct gcgtatcgcc gtcactccca cctttacaag ctactttatc ggccctttaa
    39961 tggcggattt ctatgcgcgc taccccggca tcacgctcca gctacaggaa atgtcgcagg
    40021 agaaaatcga agatctgctt tgccgcgacg agttggacgt tgggattgcc ttcgcgcctg
    40081 tgcattcgcc ggagctggag gcaattcctt tactgacaga aagtttagcg ttagtcgtgg
    40141 cgcaacatca tccgctggct gcctgtgaac aggtggcgtt gagtcgcttg catgatgaaa
    40201 aactggtcct gctcagcgcg gaatttgcca ccagagagca aattgaccac tactgcgaga
    40261 aagcggggct acatccacag gtggtcattg aggcgaactc aattagcgcg gttctggagc
    40321 tgattcgccg cacttccctt tccacattgt tgccagcagc gattgccaca caacatgacg
    40381 ggcttaaagc tatatccctt gccccgccac tactggagag aacggcggtt ttgttgcggc
    40441 ggaaaaatag ctggcagaca gccgccgcga aggcattttt gcacatggcg ttggataaat
    40501 gcgcggttgt tggcggaaat gaatcgcggt aaccactgac acaattgcct gatgcgctac
    40561 gcttatcagg cctacgagtt cagtgctcta cgtaggccgg ataaggcgtt cacgccgcat
    40621 ccggcataaa ctacgctcgc tttgtcaaca cacagcccag ccgatcaccg tttgtaatcg
    40681 atggcttctg gctgctccag atatacggtg gtttgtgctg gttgggtgct ggcaatcacc
    40741 ttaccgccac gtaccgaata acgtacagga acctgacggc gcagcgcgtc aaacccattt
    40801 tcagccggca ggataatcag gttggcgctg tttccggcgg caatgccgta atcctgcaaa
    40861 ttcaacgtcc tggcactgtg gtgggtgatt aaattcaggc catcgttaat ctgcccgtag
    40921 cccatcagct ggcaaacatg cagccccata tgcagcactt gcagcatatt cgccgttccc
    40981 agcggatacc acggatcgaa gacatcatcg tgaccaaagc agacgttaat gccggactcc
    41041 agcatctctt taacgcgcgt gatgccgcga cgttttggat aggtatcgaa acgtccttgc
    41101 agatggatat tgaccagcgg gttggcgaca aagttaatac cggacatttt cagcaagcgg
    41161 aacaggcgtg aggtatacgc cccgttatag gagtgcattg ccgtggtgtg gctggcggtg
    41221 actcgcgcgc ccattccttc acggtgcgcc agggcagcaa cggtttcgac aaagcgcgac
    41281 tgctcgtcat cgatctcatc acagtgaacg tcgatgagac ggtcgtattt ttgcgccagg
    41341 gcgaaggttt tatgcagcga ctccacgccg tattcacggg taaattcaaa atgcggaatc
    41401 gcccccacta cgtcagcccc taagcgtaac gcctcttcca gcaacgcttc accgttggga
    41461 tacgacaaaa tcccttcctg cgggaaggcg acgatttgca gatcaatcca cggcgcgact
    41521 tcctgcttca cttccagcat tgctttcagc gcagtcagcg ttgcatccga aacatcgaca
    41581 tgggtacgca catgctgaat gccgttggca atctgccatt tcagcgtttg ccaggcgcgt
    41641 tgtttcacat cgtcatgggt taataacgct ttgcgctcgg cccagcgttc aatgccttca
    41701 aacagcgtgc cggactgatt ccagttcggt tgtccggcgg tttgcgtggt gtccaggtga
    41761 atatgcggct ccacaaacgg cggtataact aaaccttgtt cggcatccag gctgttttca
    41821 gttatgggca tcaccccgga ttgcgcatca atggcgctga tttttccgtc cagcagatga
    41881 atctgccaca gcccctcttt gccgggtaac cgggcgttaa taattgtctg taaagcgtta
    41941 ttcgacactg ttagcctcca catgcgtcat tgcagccgtc gttttgcgat tcaaaatcgg
    42001 gttaaggatc agatagctca gcgcgccacc taataccgcg ttgaccggaa caatacccgg
    42061 taaccagtgg cctgcggcaa tccccaaggc gaccgccaga atcgccaccc aattgacact
    42121 catcatacgc gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat agtcagcgat
    42181 gatcacgccc cctaccggag gaatagctgc cgaaaggaag gtcagccagc cgacaaaatt
    42241 gttatacagc cataatgcgc agaccgtacc gataataccg ttgattaccg aaagggtttt
    42301 actcgacata ccagtaatgt tggcgaaacc taaacccgac gcatagagcg cgttatcgtt
    42361 ggtggtccag atattcagcc ccagcaccac aatcgcaggc agcagcaggc cctgagcaat
    42421 catcacatca gagatatccg ccatccccag tgccgcagcg cccgctgcac cgaaaataaa
    42481 catcaacgag ttgccgagga aaaaggccac catcgccacc agcaccgcca gtttggcatt
    42541 acgaccaaac cggacaaagt cagcggtgag cgtgcccgca ctgataaatg accccacaac
    42601 cagcgccagc gcgacattga aatctaacgg ttgtgcggga acgaccgctt ttaatgcgtc
    42661 caggccgccc atgccattaa cggccagcca cacggaataa ccgccaaggc aggcgatagc
    42721 cggaaccgca atcaccgaaa gaaccgtcag cgccgaaatg ccaaagaaga cggtgacggt
    42781 catcagtaaa ccggaaacgg caatcagcaa attaatatcc agcccggttg ccttgcccac
    42841 cggaatggca aacatcgcca cgccaacgcc aaaccagcca acctgggtgc cgcccagcag
    42901 cagtgaaggc agccatgagc ctttaacacc aaaagagaag cgagcaagaa gatgagtggt
    42961 caggccggtt tttgcgccaa tgtaaccaag aaatgaagtg taaataccga ggagaagatt
    43021 accgatgagg actgcgagga agaaatcatg atagctaaga ccggttccga gagtgccgcc
    43081 ggtccacata ctggcggaaa agaaggttaa tcccagcatg acgaacgtca atgccaatac
    43141 ccctttccgc gccgactgcg ggactggccc ctggctaaag ttgttatctt gcgacacgaa
    43201 attccccctt tttgctgttt gaaacccaaa aaatccgccg cattctattc atctgaagat
    43261 aaaaagcaat cgttttcgta ggggaatata tttcttatat gaatgagtac aaaaaggcgg
    43321 gggaaactta tctgtgtaaa aacagtgggt aggcatgata agacgcgtca gcgtcgcatc
    43381 aggcatctgc gca
//
LOCUS       DAZGTJ010000031        42669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000031 DAZGTJ010000000
VERSION     DAZGTJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.031"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..187
                     /gene="cdiI"
                     /locus_tag="ACHK8E_002155"
     CDS             <1..187
                     /gene="cdiI"
                     /locus_tag="ACHK8E_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFP0344424.1"
                     /translation="DESEHFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYLQL
                     HPEDTEKVNKLLSKIPS"
     gene            complement(1235..1501)
                     /locus_tag="ACHK8E_002156"
                     /pseudo
     CDS             complement(1235..1501)
                     /locus_tag="ACHK8E_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1909..3114)
                     /locus_tag="ACHK8E_002157"
     CDS             complement(1909..3114)
                     /locus_tag="ACHK8E_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0344425.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            complement(3414..3508)
                     /locus_tag="ACHK8E_002158"
     tRNA            complement(3414..3508)
                     /locus_tag="ACHK8E_002158"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3472..3474),aa:Sec,seq:tca)
     gene            3801..5183
                     /gene="yicJ"
                     /locus_tag="ACHK8E_002159"
     CDS             3801..5183
                     /gene="yicJ"
                     /locus_tag="ACHK8E_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP0344426.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            5193..7511
                     /gene="yicI"
                     /locus_tag="ACHK8E_002160"
     CDS             5193..7511
                     /gene="yicI"
                     /locus_tag="ACHK8E_002160"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP0344427.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            7807..9360
                     /locus_tag="ACHK8E_002161"
     CDS             7807..9360
                     /locus_tag="ACHK8E_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP0344428.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            9387..9857
                     /locus_tag="ACHK8E_002162"
     CDS             9387..9857
                     /locus_tag="ACHK8E_002162"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP0344429.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            9875..10189
                     /locus_tag="ACHK8E_002163"
     CDS             9875..10189
                     /locus_tag="ACHK8E_002163"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0344430.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            10214..11305
                     /locus_tag="ACHK8E_002164"
     CDS             10214..11305
                     /locus_tag="ACHK8E_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0344431.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            11317..12168
                     /locus_tag="ACHK8E_002165"
     CDS             11317..12168
                     /locus_tag="ACHK8E_002165"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0344432.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            12249..13109
                     /locus_tag="ACHK8E_002166"
     CDS             12249..13109
                     /locus_tag="ACHK8E_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP0344433.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            13157..14089
                     /locus_tag="ACHK8E_002167"
     CDS             13157..14089
                     /locus_tag="ACHK8E_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0344434.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            14070..14750
                     /locus_tag="ACHK8E_002168"
     CDS             14070..14750
                     /locus_tag="ACHK8E_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP0344435.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(14794..16503)
                     /gene="yicH"
                     /locus_tag="ACHK8E_002169"
     CDS             complement(14794..16503)
                     /gene="yicH"
                     /locus_tag="ACHK8E_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0344436.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(16624..18015)
                     /gene="xanP"
                     /locus_tag="ACHK8E_002170"
     CDS             complement(16624..18015)
                     /gene="xanP"
                     /locus_tag="ACHK8E_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP0344437.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18294..19499
                     /gene="gltS"
                     /locus_tag="ACHK8E_002171"
     CDS             18294..19499
                     /gene="gltS"
                     /locus_tag="ACHK8E_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP0344438.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(19533..21614)
                     /gene="recG"
                     /locus_tag="ACHK8E_002172"
     CDS             complement(19533..21614)
                     /gene="recG"
                     /locus_tag="ACHK8E_002172"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP0344439.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(21620..22309)
                     /gene="trmH"
                     /locus_tag="ACHK8E_002173"
     CDS             complement(21620..22309)
                     /gene="trmH"
                     /locus_tag="ACHK8E_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP0344440.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(22316..24424)
                     /gene="spoT"
                     /locus_tag="ACHK8E_002174"
     CDS             complement(22316..24424)
                     /gene="spoT"
                     /locus_tag="ACHK8E_002174"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP0344441.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(24443..24718)
                     /gene="rpoZ"
                     /locus_tag="ACHK8E_002175"
     CDS             complement(24443..24718)
                     /gene="rpoZ"
                     /locus_tag="ACHK8E_002175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP0344442.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(24773..25396)
                     /gene="gmk"
                     /locus_tag="ACHK8E_002176"
     CDS             complement(24773..25396)
                     /gene="gmk"
                     /locus_tag="ACHK8E_002176"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP0344443.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            25654..27339
                     /gene="ligB"
                     /locus_tag="ACHK8E_002177"
     CDS             25654..27339
                     /gene="ligB"
                     /locus_tag="ACHK8E_002177"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP0344444.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(27336..27953)
                     /gene="yicG"
                     /locus_tag="ACHK8E_002178"
     CDS             complement(27336..27953)
                     /gene="yicG"
                     /locus_tag="ACHK8E_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP0344445.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(28245..29069)
                     /gene="dinD"
                     /locus_tag="ACHK8E_002179"
     CDS             complement(28245..29069)
                     /gene="dinD"
                     /locus_tag="ACHK8E_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP0344446.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            complement(29291..30154)
                     /gene="yicC"
                     /locus_tag="ACHK8E_002180"
     CDS             complement(29291..30154)
                     /gene="yicC"
                     /locus_tag="ACHK8E_002180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP0344447.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            30281..30997
                     /gene="rph"
                     /locus_tag="ACHK8E_002181"
     CDS             30281..30997
                     /gene="rph"
                     /locus_tag="ACHK8E_002181"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP0344448.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            31063..31704
                     /gene="pyrE"
                     /locus_tag="ACHK8E_002182"
     CDS             31063..31704
                     /gene="pyrE"
                     /locus_tag="ACHK8E_002182"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP0344449.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(31741..32337)
                     /gene="slmA"
                     /locus_tag="ACHK8E_002183"
     CDS             complement(31741..32337)
                     /gene="slmA"
                     /locus_tag="ACHK8E_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP0344450.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(32444..32902)
                     /gene="dut"
                     /locus_tag="ACHK8E_002184"
     CDS             complement(32444..32902)
                     /gene="dut"
                     /locus_tag="ACHK8E_002184"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP0344451.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(32880..34100)
                     /gene="coaBC"
                     /locus_tag="ACHK8E_002185"
     CDS             complement(32880..34100)
                     /gene="coaBC"
                     /locus_tag="ACHK8E_002185"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP0344452.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            34272..34940
                     /gene="radC"
                     /locus_tag="ACHK8E_002186"
     CDS             34272..34940
                     /gene="radC"
                     /locus_tag="ACHK8E_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0344453.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            35157..35393
                     /gene="rpmB"
                     /locus_tag="ACHK8E_002187"
     CDS             35157..35393
                     /gene="rpmB"
                     /locus_tag="ACHK8E_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP0344454.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            35414..35581
                     /gene="rpmG"
                     /locus_tag="ACHK8E_002188"
     CDS             35414..35581
                     /gene="rpmG"
                     /locus_tag="ACHK8E_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP0344455.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            35679..36488
                     /gene="mutM"
                     /locus_tag="ACHK8E_002189"
     CDS             35679..36488
                     /gene="mutM"
                     /locus_tag="ACHK8E_002189"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP0344456.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(36527..37006)
                     /gene="coaD"
                     /locus_tag="ACHK8E_002190"
     CDS             complement(36527..37006)
                     /gene="coaD"
                     /locus_tag="ACHK8E_002190"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP0344457.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(37014..38291)
                     /gene="waaA"
                     /locus_tag="ACHK8E_002191"
     CDS             complement(37014..38291)
                     /gene="waaA"
                     /locus_tag="ACHK8E_002191"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP0344458.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            38704..39762
                     /gene="rfaQ"
                     /locus_tag="ACHK8E_002192"
     CDS             38704..39762
                     /gene="rfaQ"
                     /locus_tag="ACHK8E_002192"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP0344459.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            39759..40883
                     /gene="waaG"
                     /locus_tag="ACHK8E_002193"
     CDS             39759..40883
                     /gene="waaG"
                     /locus_tag="ACHK8E_002193"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0344460.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            40876..41673
                     /gene="rfaP"
                     /locus_tag="ACHK8E_002194"
     CDS             40876..41673
                     /gene="rfaP"
                     /locus_tag="ACHK8E_002194"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP0344461.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            41689..>42669
                     /gene="waaO"
                     /locus_tag="ACHK8E_002195"
                     /gene_synonym="rfaI"
     CDS             41689..>42669
                     /gene="waaO"
                     /locus_tag="ACHK8E_002195"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFP0344462.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKLIEAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMN"
BASE COUNT        10430 a        10230 c        11118 g        10891 t
ORIGIN      
        1 tgatgaaagc gaacattttg aaggtgttga atttgctgtc ggctatcccc cagatgaaga
       61 tgatatagtg atcgtaagcg aagaaacctg ttttgaatat gttcgtctgg cctgtgaaaa
      121 atatcttcag ttacaccctg aagatactga aaaagtaaac aaattactgt ctaaaatccc
      181 atcctgagta aagattccgg ccactgcgcc gggatctttc tttaaccgtc aggctcatcc
      241 acaatgtcca tccccacgct taatcaccgg cacatctcta atgtcgttaa gcgcaccggg
      301 gaaggcgtta aaccacgtga gcatctgcgg cctgttgaca tacgttgtgt cgttacgggg
      361 aagtcatctt cacagcgatt ttcagtcagt tagcattacc gacattcatt catcaaatta
      421 ccggagataa ttgatgtcaa aaccgtaagc gtaaactgac cgccgtatgt agccatcaga
      481 cgagaattgg taacttagac gcccatctga tatagacgga catctaagta tggaattaca
      541 ggactggcga aaagaacctc gtaaaaacta ttcgaatgaa ttcaaacttc gtatggtgga
      601 actggcatca caacctggag gtttatccca agaatattgc ctttgatatg ctggcacata
      661 caatccttga ccattctcac agatatactc ccacacactt ttgggttgcg ccccgaaaac
      721 atctttgatt tccatataac cccttgattt aataccattt atcaaaagtt taacatttcg
      781 gaattttacc aactatggaa gacttagcaa taaccatcaa acaatactaa ctaatcatcc
      841 cttagcaagg gctaactcta cgaaacgcta tgctgcttct tcgagttctc cattgttttt
      901 tcgctttact gttggaaaaa taaaaattaa aaaacatata ttaacattat ccataatcta
      961 gcatataaat aaagcgaaac aaatattttt ttacttattt taattagtag aggttcgtat
     1021 gagtaaaaat aactttagta aaagtcccta ttatagaagc cgtagcaaaa cgaaatttta
     1081 acagaagcaa cttcattttg taagtgtccg gcttaataat gaagagctca cgcttctaaa
     1141 cgataaacgt ggtagccatc ggcttccttt attttttctt aatgtgcttc ctagtattgt
     1201 tccagtcatt aatatagaag catgggatct ttagcaggct tttcacagaa ataaataaaa
     1261 atattgccca ccttggctgc tccagaaatg catatgccag atgagcgtgt atatcaataa
     1321 agtcaggctc ccgttgggca agctacctga gtttgttgat ataactcgtc tcgctgattt
     1381 catcagtgcc gtatgcgtca tgcacttctt caaactcctc cagtaatggg ccaaacctgg
     1441 tattcggaaa tacgaaacgg ccttggttat cgttcacatc aaagcgtaat ctgtcgttca
     1501 ttttctgagc ctgtaaaacg gatgggacac aatctgaaaa gcataaaagt cgtgatgttg
     1561 tgtatcaagt cttgttattg cccgggatga atattgttta caatatcatc gacagtaatt
     1621 cttcatgtgg ataaaaattt atctttatct gcattccaat gcattgtgaa tagtcggcta
     1681 agacaacatg ttagttgatt tttggtgtga tggtgctgaa ataacgacag gatgatgatt
     1741 gaaattacag gcatcaaaat tgttagcggt ctatagcggt gtgatgttct gagattgttt
     1801 gcagatgcaa tataaatatt ttgtcaaaaa atatctaaaa ataaaacatg aaaacaaaat
     1861 tcctccagac ctggaggccg gaggaatttt gtgaattgag agaaaatatc agtacaaggg
     1921 gccttgacac tgactctcgt ggctggaaag cgaatcaatg taatcagcgt accattgcat
     1981 catttcacgg cgaccatcta gatacagggc atgattatag gtccccctga tagcgttttt
     2041 atctacatga gctaactgcg tttcaatcca agcggaatta aagccctgtt cgtgtagaat
     2101 ggtactcatg gtgtgtctga aaccgtgccc tgtgagtctt cccttatatc ccagtaattc
     2161 aatgacttgg ttaatgcttt ccttactaat tggcttgcga ggatcgttcc taccgataaa
     2221 gaccagagga tgatgttttg tgatgggttc aagttgctta aatagggcaa tgacctgctc
     2281 agagagcggc acaatatgag gtcgcttcat cttcattact tctgccggaa tctcccataa
     2341 ttttgtctta aaatcgatat cctcccagcg tgcaaaacgt aattcttgtg ttcgtacacc
     2401 ggttaacata atgatctgag tagctgtttt agtgatgata cttccggtat aacccgccag
     2461 atcgttgagg aagtggggaa gttcattagc agtaagaaag ggaaaatgta cttttttcgg
     2521 tgtagctaaa gcacaggcaa gatcgggagc agggttgtag tctgcccgac cagtaacaat
     2581 tgcatagcgg aacacctcac cacagcgctg gcggactttg cgcattttct ccagagctcc
     2641 acgtttttcc attttacgca gtgcttccag taactccatt ggtttgattt cggcgatagg
     2701 gcgtttgcca atgtagggaa aaatgtcttt tttaaaggta tcaatgattt catcacgata
     2761 gcgtaatgac caccgatcgg cttttgactg atgccattcg cgagcaatgg cctcgaacgt
     2821 atttttcaaa ctcatttgct gcgcgatttt ttcttctttt tttgccaccc ctggatcgcc
     2881 tccagcagcg aggatttttc tggcttcgct gcgtttttca cgagcgtcgg ctagcgttac
     2941 atcggggtat acgccaatag aaagcatctt ttctttacca gcaaagcggt acttcatacg
     3001 ccagtattta gaaccattag tgttaatcag tagatacata ccgccgccat cagcaagttt
     3061 atagggcttt tcttttggtt ttgcggtttt cactttaata tcggttaatg ccatgacatc
     3121 ctccggcttt taatgaattc atgttcctga gggggatttc tgccagtaat taacctgata
     3181 cccccaatgc accccttgta taacaaagat gttgttgaaa gccgttgtta tatatcatat
     3241 tttaccaatt atgggggtgc tgatatttca ataggaaata atacccccta aagtaccccc
     3301 aaaagcaact tgatacgggg tgaagtcggt tgacttctgg atacaaagga atcctgatat
     3361 acaagggatt agaagggggg gattgacatc gggagagttc agaagatgtt aattggcgga
     3421 agatcacagg agtcgaacct gcccgggacc gctggcggcc ccaactggat ttgaagtcca
     3481 gccgcctcac cggagacgac gatcttccgc gcctcgattg ctacatggag gcggggcgca
     3541 ttatagctac ttccttgagt ttctacatcc cccagatcag ttttttccgc ctaatacacg
     3601 cagcgtatta tcacgaaaac attatagttc atataaaact catattttac aatgatatat
     3661 gagcgaaatt aacgctacca cgatcacata aaacaagaat cataaattaa taaccagata
     3721 tcggaatatt cgctctcgca gggatggttt acaaaatgcg ttatcggtct cagcacccct
     3781 atagcatcaa ggaaaagcag atgaagagtg aagtgttgtc cgttaaagag aaaattggtt
     3841 atggcatggg agacgccgcc agccacatta ttttcgataa cgtaatgtta tatatgatgt
     3901 tcttttatac cgatattttt ggcattcctg ccggatttgt cggaaccatg tttttggtcg
     3961 ctcgtgcact ggatgcgatt tccgatcctt gcatggggtt gttggccgat cgaacgcgct
     4021 ctcgctgggg taaatttcgt ccgtgggtac tgtttggcgc actgccattt gggatcgtct
     4081 gtgtactggc ctatagcacg ccagatctca gtatgaacgg aaaaatgatt tatgcagcaa
     4141 ttacttacac cctacttacc ttactttata ccatcgttaa tatcccttac tgcgcattgg
     4201 gtggtgtaat caccaatgac ccgactcagc gtatctcgct gcaatcctgg cgttttgtgc
     4261 tggcgaccgc gggaggcatg ctctctactg tgctgatgat gccgctggtt aatttaattg
     4321 gtggtgataa taaaccactc ggtttccagg gcggtatcgc ggtcctttcc gtggtggcat
     4381 tcatgatgct ggcattctgt ttcttcacca ctaaagaacg cgttgaagca ccacctacaa
     4441 cgacgtctat gcgggaagat ttacgtgata tctggcaaaa cgaccagtgg cggattgtcg
     4501 gtttactaac cattttcaat attctggcgg tgtgcgtacg cggtggagcg atgatgtatt
     4561 acgtcacatg gattttgggt acgccggaag tgtttgtcgc ttttctcacc acttattgcg
     4621 tgggtaacct gattggttcc gcactggcaa aaccgctgac cgactggaaa tgtaaagtca
     4681 ctatcttctg gtggacgaac gccctgctgg cagtgattag cctcgcgatg ttctttgttc
     4741 ccatgcaggc cagcatcact atgtttgtct tcatcttcgt gattggtgta ttgcatcaac
     4801 tggtgacacc tatccagtgg gtaatgatgt ccgataccgt cgactacggc gagtggtgca
     4861 atggtaaacg cctgaccggg atcagttttg ctggcacgct gtttgtgctc aagctggggt
     4921 tggccttcgg cggcgctctt atcggctgga tgctggctta tggcggatat gatgcggcag
     4981 aaaaagcgca gaacagcgcc acgattagca tcattatcgc gctattcacg attgttccgg
     5041 cgatctgtta cttgctgagc gcgattatcg ctaaacgcta ctactcactc acgacgcaca
     5101 atctgaaaac cgttatggaa cagctggcgc agggcaaacg ccgttgccag caacaattca
     5161 cctctcaaga agtgcagaac taaggaacgg caatgaaaat tagcgacgga aactggttga
     5221 ttcaacctgg cctcaatttg attcacccgc ttcaggtgtt cgaggttgaa cagcagggta
     5281 atgaaatggt ggtctatgct gccccccgtg atgtgcgtga acgtacctgg cagcttgata
     5341 cacctttatt tacgctgcgc tttttctccc cacaggaagg tattgttggt gtacggattg
     5401 agcattttca gggggcgctg aataacggtc ctcattatcc gctcaatatt ttgcaggatg
     5461 tgaaggtcac aatcgaaaac acagaacgtt atgctgagtt taaaagtggc aacttaagcg
     5521 cgcgtgtcag caaaggtgag ttctggtcac tggattttct gcgcaacggc gaacgtatta
     5581 ccggtagtca ggtgaaaaat aatggctacg tgcaggacac gaataatcaa cgaaattata
     5641 tgtttgagcg gctggatctt ggcgttggcg aaacagttta cggtctggga gagcgcttta
     5701 ctgccctggt gcgcaatggc cagacggtag agacctggaa ccgggacggc ggcacaagta
     5761 ctgaacaggc gtataaaaat atcccgttct atatgactaa ccgtggttat ggggtactgg
     5821 tcaatcatcc tcaatgcgtc tcttttgaag tgggatcgga gaaagtctcc aaagtgcagt
     5881 tcagcgttga gagtgaatat ctcgaatact ttgttatcga cggcccgacg ccgaaagcgg
     5941 tacttgatcg ttatacccgt tttactggtc gtccggcgct gccgcccgcg tggtccttcg
     6001 gcctgtggct aaccacttca tttaccacca actacgacga agcgacggta aacagcttta
     6061 tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt ccactttgac tgtttctgga
     6121 tgaaagcctt ccagtggtgc gattttgagt gggacccgct gactttcccg gacccggaag
     6181 ggatgatccg ccgcctgaaa gcgaaagggc taaaaatctg cgtctggatt aacccctata
     6241 ttgggcaaaa atcccctgtc tttaaagagt tacaagagaa aggctattta ctcaaacacc
     6301 cggacggttc gttatggcag tgggataaat ggcagccagg tctggcaatt tatgacttta
     6361 ccaatccgga tgcctgcaaa tggtacgccg acaaactgaa aggtctggtc gcgatgggcg
     6421 ttgattgctt taagaccgac tttggcgaac gtattccaac cgatgttcaa tggtttgacg
     6481 gttccgatcc gcagaaaatg cataaccatt atgcgtacat ctacaacgaa ctggtgtgga
     6541 acgtgctcaa ggacaccgtt ggtgaggaag aagctgtctt gtttgcccgc tcggcttccg
     6601 tcggtgcgca gaaattcccg gtacactggg gtggcgactg ttacgctaac tacgaatcaa
     6661 tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc aggttttggc ttctggagcc
     6721 acgatatcgg cggctttgag aataccgcgc cggcgcatgt ttacaaacgc tggtgcgcgt
     6781 ttggtttgct ctccagccat agccgtttac atggcagcaa atcttatcgt gtgccgtggg
     6841 cctacgatga tgagtcctgt gatgtggtgc gcttcttcac gcaactgaaa tgccgcatga
     6901 tgccgtatct gtatcgtgaa gctgctcgtg cgaacgcgcg gggtacgccg atgatgcggg
     6961 ccatgatgat ggagtttccg gacgatccgg cttgtgatta ccttgaccgt caatacatgt
     7021 taggcgacaa cgtgatggtt gcgcctgtgt tcactgaagc gggcgatgtg cagttctact
     7081 tgcctgaagg tcgctggaca cacctgtggc acaacgatga actcgatggt agtcgctggc
     7141 ataaacagca gcacagcttc ctgagtctgc ccgtttatgt gcgtgataac accctactgg
     7201 cgctgggcaa caacgagcaa cgtcccgatt acgcgtggca cgaaggcacg gcattccagc
     7261 tctttaatct acaagacggg catgaagccg tctgtgaaat gcccgctgct gacggttcgg
     7321 tgatctttac tctaaaagca acacgtactg gtaacacgat tactgtgact ggtgcgggcg
     7381 aggcgaagaa ctggacactg tgcttgcgca atattgtgaa agtaaatggt ctgcaaggcg
     7441 gttcgcaggc tgaaagtgag caggggctga tggtgacgcc tcaagggaat gcgctgacaa
     7501 ttacgttgta atctattgat ggaatgccgg gtacgaatgg ttttgtgtcg tatccggtaa
     7561 ataatctcac atctggtaat aaatatttct tccatcgtat ttgcgagcat gatcacgatt
     7621 attcatcgcg aaaatgtgat ctgtgctgaa aaatatccct taaccgcctt gctttgtttt
     7681 atcactgtcc ggattacgga cagattttag tattgttgtg aaattattct gctttatatt
     7741 taccctcaga gatggcaact gaagctcttc ctcatgaggc ttcttaatct gtgataaggg
     7801 taaaaaatgc aaatgattac ctcgcgacaa aacagattat tacgattcct tttacctcga
     7861 agggaatata cgactattgt tacgattgcc ggctatttaa atgtttcgga aaaaaccatt
     7921 caacgtgatt tacgtttact tgagcaatgg ctgggacaat ggagaataaa tgttgagaag
     7981 cgtgctggcg cgggtgtgat gttaagcgcg gagaatatta ctgatttgct gcatcttgat
     8041 catttgttgg tggcagaatg tgaagagatt gatggtgtaa tgaataattc caggcgcgtt
     8101 aaaatagcgt cgcagttatt aagtgaaaca ccgaatgaaa cgtcgatcag taaattgtca
     8161 gaacgctact ttatcagcgg agcctctatt gttaatgacc tgagagtaat tgagtcctgg
     8221 cttgcgccgt tggggttatc gttgatccgc agtccaagtg gtacgcatat tgaaggtagt
     8281 gaagggcaag tccgacaggc aatggcatta ctgattaacg gcattattaa ccataatgag
     8341 ccgcaaggtg tcgtgtattc acgtctggat cccggaagct ataaagcatt agtccattat
     8401 tttggagaag aagaggtgtt atttgtccag tcattgttac tggatatgga aaatgaatta
     8461 agttggtctt tgggagaacc ttattacgtt aacattttta ctcacatcct tattatgatg
     8521 tatcgcaaca cgcacgggaa tgcgttatca agagaagaag atcaaaccag gcaatatgat
     8581 gaaaatatct ttaatgttgc cagtcagatg attcataaga tagaacaacg aattgcacat
     8641 acattgcccg atgatgaagt ctggtttatt tatcaatata tcatttcatc aggtgtggcg
     8701 attgatggac aaaaagatgt tagcattatt tcacatatgc aggccagcaa tgaagcgcgt
     8761 ctgattacct ggcgtttaat tacggtattc agtgacatcg tggactgcga ttttagtgaa
     8821 gacagcgcat tatatgatgg cttattggtg catattaaac cgctgattaa ccgactaaat
     8881 tatcgtattc atatccgtaa tccattgttg gaagatatta aagcagaact agcggatgtc
     8941 tggcggttga cgcaatatgt ggtgaatcag gtatttaaaa cctggggtga gaatgcagtg
     9001 agcgaggatg aagtaggtta cctgaccgtt cattttcagg ctgcgatgga gcggcaaatt
     9061 gcccgtaaac gcgtattact ggtctgttca accggaatcg gaacttcgca tctactgaaa
     9121 agccgtattc tgcgagcatt tcctgaatgg acgattgttg atgttatttc agcagcgaat
     9181 ttatcacagg ttttgcctga caatatcgaa ctgattattt cgaccattaa tttgcctaca
     9241 gtcactatgc cggtcgctta tgttaccgct ttttttaatg atgccgatat taagcgggtc
     9301 actgaaatgg tgattacgga aaaattacat catgcgacat ctcgggtcgt tgaaatttaa
     9361 tgtcagaatt ttatgaggta tgacgaatgg acataacaaa aatcctgaat acgaatcgcg
     9421 ttattttaga tatgcaagcg acgaataaag aggaggcgat tgaagaatta actaatcttc
     9481 tcaaaaaaga tggtgcgatc gattgtcggg aaacctttat taaagatgtc tggcagcgcg
     9541 aagcggaagg ctcgactggt tttgaaaatc atattgcgat cccgcatggt aaatcttcag
     9601 ctgtgattaa tactacgctg gcaattggcc gtacccggca ggatattccc tgggaaacgc
     9661 tggatggcag tagcgtgcgc tgtattattt tatttgcggt acgtcttgaa gatcaaaata
     9721 ccacccatat ccgtttactt tcccaggtcg cgggcgcgct ggcggatgat gacatcatag
     9781 aacaattgtt agttgagacg agtccgcaaa aaatcattga tctctttagt cagtatgcac
     9841 aatccgatgt ctgctaaata acagggagta tgtcatgaat attgtctgtg ttgccgcctg
     9901 tacggcgggt attgctcata cctatattgc tcgtgaaaag ttaatcaaag gagcaaaagc
     9961 tctgggtcat accattaaag ttgaaaccca gggaaccatt ggtacagaaa atgaattaca
    10021 ggcggcagat atctctgctg ctgacgtcgt gattcttgct gtagatgtga aaataaaagg
    10081 agaagaacgc ttcaccaata aacgtattgt gcgagtaaag acagagatcg tcattaaatc
    10141 gcccgttcaa tttcttgaaa aggtcgaaaa atcactggga aacaaatgat ccttatactg
    10201 gagggatctt aatatgaaag aaaataaaat tccggtttca caggaaatta aaaaacattt
    10261 attaaccggc atctcgtgga tgatacccct tattgtggcc gccgggatat gtattgcgtt
    10321 ggggcaagtt cttggtggta cagatgttgg tgaaaaaaca ggaacaatcc cgtggatgct
    10381 taatcagatt ggcggctggg gaatggggct gattgtgccg ctgattagcg cggcaattgc
    10441 ctattctatt gccgatcgtc ccggatttgc cccgggcctg attgtcggct ttctctgtgg
    10501 gcaaatccat accggattta ttggcggtat gttgggtggt ttcctggtgg gttacaccat
    10561 tttgctactt aaacgctata tccgcctgcc gcagtcgatg caaggactca tgccaatcat
    10621 ggtgttgccg gtattaagca ccattatcgg tgggctgtta atgatgacgc tgattggtaa
    10681 acccatcgcc tggttacaag aagcactgat tcatttgctc gagtcgatgc agggcggatc
    10741 gcgcttcctg atgggggcca tacttggcgc gatggcaacc ttcgatttcg gtggtccggt
    10801 caacaaaacc atgtcgctgt tttccgatgg cttgctggtc agtggcgtct acggaccaga
    10861 agccgtgaaa tttgtcggct caatcatccc tccgtttggc atcacacttt cgttcttact
    10921 gacacgccat aaatacaccc gtgcggagcg cgaggcgctg aaagccgcgt ttccgatggg
    10981 gatctgcatg atcactgaag gagtcattcc gatcgccgcg cgtgacctgc tgcgagtagt
    11041 gggatcttgt gtggtggcat cggcggtcgc gggcggctta atcatggtgt ggggcgtgga
    11101 gagtccggtt ccgcatggcg gcatgtttgt tgtgcctctg ttcacccatc ctctgctgtt
    11161 ttgcctggcg ctgggtatag gcacggtgat ttgtggcgtg atgctttcac tctggaagaa
    11221 accggtaacg gaacgcgacg aagagtttga cgaactgagc gatcaaaagc tgaaagatga
    11281 agagatcact ttcaccctcg aataaccgga ggccctatgt ttgccgatat gaaaagtatg
    11341 gtgatgaaag cctggcatga acattacgct ttgctggcga tcaactgtat gaatctggaa
    11401 agtgctcacg cagctatccg cgtggcggag aagaatcgcg ctccgatcat tctcaatctc
    11461 tatcaggggc atctggcgca ttttcctgca cccgtcgcgg cggcagtggt taaaacgctc
    11521 gcggaatctg cctcagtacc ggtcgctctg gcgcttgatc atggcaaaaa tccggactgt
    11581 attcgtcagg cgtttcgtgc tggtttcagc ggattaatga ttgatgcttc tgcttttccc
    11641 ctggaggaaa acgtgcgcca gaccagggcg gtggtggaac tcgccgccag cgtgcagctc
    11701 tgcgtggaag gcgaactggg ccatctggca gatgcgccgc gttacgacca ggccgccaat
    11761 gcagacctga tgacgcaacc cactgatgtg gaaacgttta tcgcgcagac aggcattgat
    11821 ctactggccg tctcggtagg cacggcgcac ggcatgtacg cgcctggtgt tgtacctgct
    11881 attgattttc agcgtctggc ggagatttcg cgctgctcga gcgtgcctct ggcattacat
    11941 ggcggtagcg gtacgccgtt cgatcagctc cagctctgta cgacgctggg ggtagcaaaa
    12001 atcaacgtgg gggcggccat ttttgaacgg gggaaatccg cgctcctgca taccctctgt
    12061 cacgatatat ccattgaact ggtggacgcg ctgaaagcta tggaactggc cttcgaagaa
    12121 gcgattacac cgtatttaca ggcgagcggc gctatcggca aagcctgatt cattttctgt
    12181 catggccttt atgccagtgc aataacgcac cggcagggcg gactacacgc tgaaactgga
    12241 gatttaacat gttagtttcg atgaaagaat tactgcaacc tacccgccag cacggctttg
    12301 ccattggcgc atttaatgtg gcggataact gtttccttcg cgcagtcgtg gatgaagcag
    12361 aagcgacaaa tactccgacg attattgcca tccatcccag cgaacatgat tttgtgggcg
    12421 atgccttttt cgctcacgtt cgcgaaatta cccagcgtag tccggtcccg tttgtactgc
    12481 acctcgatca tggtgcctct gtagagcatg ttttgcgtgc gattcgctgc ggatttacct
    12541 cagtaatgat cgacggttcg ctgctgccat atgaagaaaa cgtggcgctg accgcagaag
    12601 tggttcgcct cgctcacgcg gttggagtat cggttgaggg tgaactggga accatcggac
    12661 aaacaggaac gtcggtagaa ggtggtgttt cacaagtgac ctacacagat ccggcgcagg
    12721 ccgccgattt tattgctcgt acaggtgccg acacactggc tgtggcgatt ggtacagcgc
    12781 atggcatcta tccaaaaggg atgcagccga agctgcaaat ggacattttg cgcgacattg
    12841 ccggacgtat tgatattccg ctggtgctac acggtggttc ggcaaacccg gacgcggaga
    12901 ttgctgaatc agttacgctc ggcgtcggca aaatcaatat ctccagcgat atgaaatacg
    12961 cctatttcca gaaagtgcgt gaaattctcg ctaaagagag ctggtgggac ccgaacgtca
    13021 tctatccgga tgcgattaac gcagcgcggg aagtcattcg ccacaaaatg aaactgttcg
    13081 gttcgctcgg taaagcatca ctgtactaag ccagaagggc ggggtgaccc gccttttacg
    13141 caacaaggaa cgagatatgc actatctggg gttggatatt ggtgggacca aaatcgccgc
    13201 cgtcgtcatg gatgcgcatg gctgggagat tcgccgttac cgctgcccga cgcaaaagtc
    13261 gacatatcaa caatttgtct catgcgttgt ggcgcttatc gagcagatta ggcgggacgt
    13321 tcaacgaccg atgctgacag ggatcgcctt acccggcagt atctcgccac tcactggcct
    13381 gattaaaaac gcgaatattc aggtgattaa cggtcatgcg ttacaggctg atttgcagca
    13441 attgcttggg caaccggtgg tgatagccaa tgatggtaac tgtttcgcgc tatcagaagc
    13501 ttgcgacggt gccgggcaag attatgacgt ggtatttggt attacgcttg gtacgggctg
    13561 cggcggtggc attgccatca agcaacgacc gtttataggg gcctggggaa atgctgccga
    13621 atgcggtcat atcacgttgc caggctatac ggagcaggaa gatggtccat cagtcagttg
    13681 ctattgcggc aaacacaact gcgtggagtc gtttgtttcc ggcagcggtt ttagtgaacg
    13741 ctatcaacag atgactggta acttgctcac tcctgcggcg attgtcaccc tggcacaacg
    13801 tggtgatgct tgtgccatgc agcaggtggc acgttttcgc caacagcttg cccgcacgct
    13861 ggcaaccatc gttaatgttg ttgaccctgg cgtgattgtg atcggcggcg ggctttcgaa
    13921 tgtggaactg cttatcaccg atctgaacgc agaagtcgct ccactggttt tcaccgatca
    13981 attcaccact cccattgtaa aagcacagca cggcgacagt agcggaatgc gtggcgctgc
    14041 ctggcttgct atgcgcaacg gagaagccaa tgaaacgttc acaaattaat tatgttattg
    14101 ataaagcaca tgccattgcg cagaccttcc gcgtttgttt gccagagttc gcctatttta
    14161 cggtggatga ctggctgcag cgggaacggg ataactggca agaagtggtc gacttacagc
    14221 tcggttggga tatcaccgat tttggtcgtg gtgatttcaa ccaaacggga ctaacactgc
    14281 taacgatgcg caacggcgcg ctggggtcgg cggcatatcc aaaaccgtat gctgagaaaa
    14341 tgttgcagat ccaacaagat cagcagactc cctggcattt tcacacccac aaaatggaag
    14401 atatcctcaa tcggggcggt ggagacttat gcatgcagtt ggcctgggcc aacgaagcca
    14461 atctctgtga tgatcaacga gtcattaccg tgagtgttga tggtcagcgg cgtaccatga
    14521 aacctggtga aacgctggtg ctaaagccgg ggcagggcat ttgcctgccg ccgcgtctgt
    14581 atcatcgctt ctgggcggaa aaagcgtttg ttctcggctg ggaaatctcg atggtaaatg
    14641 atgaccaaca cgataattat tttcttgagc cgggaggacg ttttccggcg atagaagaag
    14701 atgaaccggt gaaatggtta ttgtgcagtg agtatggaat tttgcgctaa taacggggaa
    14761 cgctagcgct gatatccgct agcgtcctta tttttactgc accggctccg tcgacgtaac
    14821 ttcaccactg gaaactacgc ccgcattcat ggtttgagtc acttgctgct ttgcggcatt
    14881 cacggcatgc aattgcccgg taaccgtagg tttcagcggg acattggcct gaatatcgcc
    14941 actggcagta agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccactg
    15001 ttgcaaaata ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg
    15061 cgtttgtgaa acactggcgg tggcttccag aatgcctttt tcagtaaatg cactcagttc
    15121 gctgatattc accgtgctgc tgttggcggt cagcgccagc gatgggcgac gaacatcaac
    15181 acgattgaat gtcgcggcgg cggcattcag attcgccgaa ccactccaga cgccccattt
    15241 atgatcggta accagcgtca ggttggcacc gtaaccatcg agcgcggtga gctgccacgg
    15301 gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag
    15361 gctatttaac caaccgggtg tcgtttccat ccacaactgt tgccagtttt ttggcaaggt
    15421 atattccagc ccggcaattg ccgcatcatc aaggagtaac gttttcccgt cacgcaacca
    15481 gctccctgac gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac
    15541 gccctgcggg gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat
    15601 gaactcgcta gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat ctttactgaa
    15661 ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcaaacg
    15721 agcgtcaatg acttccaggc gaccaatctg caacgacggg acagagcgta atggcgcaaa
    15781 gaagtcggtt agcgattttt cgctttgtag acggatatcc gccatgcgca gattttccac
    15841 ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc
    15901 ggcacccaag ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt
    15961 ggtggcagga acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt
    16021 acccagcact ttaccggctt ctggtgacca cggaaccacg ccaccattta cccgctgcgc
    16081 gcttagtttc cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc
    16141 ggctttgaac ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcaa
    16201 gatggtatcg acatggcgtg gttcggtcag ttggcgactg cttagcgcaa tgtcgacact
    16261 tttggccacc agggtcgcgg gctgaccatc acgaccaaac gtgacgttct ccagcacgat
    16321 atgagatggc gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt
    16381 ctcggaaacc catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag
    16441 gccagcgatc accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt
    16501 catggtcttc catcccgtga aaagcacata agggagttat gcacgattta cgcgcaatcc
    16561 tcaaggcggg aatggtgaaa gacgtgagac tgcggcggta attatcttac cgccgccagt
    16621 gaattactgt ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc
    16681 ggcaatcccg gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg
    16741 ctgctgagag acgcccagac cgaccgccag cgacagcgcg ataatcagaa tcgcccgacg
    16801 gttcagcggc tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac
    16861 aagcgttgcg ccgcccagaa ccggttcagg aatgtgttgt acaaaaccgc tcactgccgg
    16921 gaacagaccc agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac
    16981 accggttaac tggatcactc cgttgttctg cccgaagcag gagttcggga aggtgttaaa
    17041 caccgccgaa acaaacgagt tcaggccgtt tgccagcaca ccgcctttca ggcgtttcat
    17101 gtacagcgga ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat
    17161 ggtttccagc gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat
    17221 gccaagaccg taatagagcg gcgttggcac cataatcagt tcttgtgtca tcggttcgtt
    17281 gctttctggc aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaattac
    17341 cagtgaggcc acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa
    17401 aaccacgcct gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc
    17461 gtaaccgccg ccaatggacg ttaacccaac ctgaattagc gacaggccga taatcatcac
    17521 cacaacgcca gaaactagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat
    17581 caccatctca gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg
    17641 aacatcagca ccaccggttt tcagcgcggt accgcccata atcagtgggg caacaaagtt
    17701 gaagctggtg ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg
    17761 aataatcgat gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg
    17821 tgccggtaaa cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat
    17881 cgccagcaga tgctgacagg cggcaaacag agtttgagga agcggcggac gatcttcaag
    17941 acggtaaatc agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag
    18001 ggtggaaaca gacatcgaaa acaatcccgt tgtagcaaag cgggcatttt agctgaccga
    18061 aagagaaaag caaacgtttg ccacggcctc tgctaaatat ttaaatgaga taaatgcact
    18121 tttttatata acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc
    18181 tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcataa gttgccactg
    18241 cgcgatgaag tatgacgagt atgaaagagt gatgcggata caaaggagta actatgtttc
    18301 atctcgatac tttagcaacg cttgttgccg caacgctgac gttgctgctc gggcgtaagt
    18361 tggtccattc cgtctccttt ttgaagaaat acaccatacc ggaacctgtt gcgggtggtt
    18421 tgttggtggc gctggcgcta ctggtactga aaaaaagcat gggctgggaa gtcaactttg
    18481 atatgtccct gcgcgatccg ttaatgctgg cattcttcgc caccattggc ctgaacgcca
    18541 acattgccag tttgcgtgcc ggtgggcgtg tggttggcat cttcttgatt gtggttgttg
    18601 gtctgttggt gatgcaaaat gccattggca ttggtatggc tagcctgtta gggcttgatc
    18661 cgctgatggg gctgttggcc ggttctatta cgctttccgg cggtcacggt acgggcgctg
    18721 cgtggagtaa attgttcatt gaacgctatg gcttcaccaa tgcgacggaa gtggcgatgg
    18781 cctgtgcgac gtttggtctg gtgctgggcg gcttgattgg cggtccggta gcgcgctatc
    18841 tggtgaaaca ctccaccacg ccgaacggta ttccggatga ccaggaagtc ccgacggcgt
    18901 tcgaaaagcc ggatgtgggc cgcatgatca cctcgctggt gctgattgaa actatcgcgc
    18961 tgattgctat ctgcctgacg gtggggaaaa ttgttgcgca acttttggct ggcactgctt
    19021 ttgaactgcc gaccttcgtc tgtgtactgt ttgttggcgt gattctgagc aacggtctgt
    19081 caatgatggg cttttaccgc gtctttgagc gtgcggtatc cgtgctgggt aacgtaagcc
    19141 tgtcgttgtt cctggcgatg gcgctgatgg ggctgaaact atgggagctg gcttcgctgg
    19201 cgctgccgat gctggcgatt ctggtggtac agaccatctt catggcgttg tatgccatct
    19261 tcgttacctg gcgcatgatg ggcaaaaact acgatgcggc agtgctggct gcgggtcact
    19321 gtggttttgg cctcggtgca acgccaacag caatcgccaa catgcaggcg atcactgaac
    19381 gttttggccc gtcgcacatg gccttcctgg tagtgccgat ggtcggtgcg ttctttattg
    19441 atatcgttaa cgcgctggtg attaagctgt atttaatgtt gccgattttt gccggttaac
    19501 cgatgaagcg gcggtcttct caccgccgcc ttttacgcat tcgagtaacg ttccgtttct
    19561 ggcatccagc gttctatcag ggcttttgcc tgctgtgggt aacgttcgtg aatatggcgt
    19621 gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc cactttaaat
    19681 tcagcattac ccgtctgacg cgtgcctaac aattcgccag ggccgcgaat ctccagatct
    19741 ttttgcgcaa tcacaaaccc gtcgttgctg tcgcgcagta cttgcagacg aatttgcgcc
    19801 gttttagaga gcggcgtttt gtagagcagc acgcagtgag aagccaccgc accacgtccc
    19861 acgcgcccgc gaagctggtg caactgcgcc agacccagac gctccgggtt ttcgataatc
    19921 atcaggctgg cgttaggcac atcaacgccg acttcaataa cggtagtggc aaccagcagg
    19981 tgtaactcgc cttgtttaaa tgatgccatg acggcctgct tctcggctgg tttcatccgt
    20041 ccgtgtacca gaccaacatt caactctggt agcgccagtt tcaactcttc ccaggtagct
    20101 tccgccgcct gcgcttccag taactctgac tcttcaatca acgtacaaac ccagtatgcc
    20161 tgacgacctt cagttatgca ggcgtggcgc acgcggtcaa taatgtcggt acggcgggta
    20221 tcaggaatag cgaccgtagt cactggcgtg cggcctggcg gcagctcatc tatcaccgag
    20281 gtatcgagat cagcatacgc agtcattgcc agcgttcggg ggatcggcgt ggcggtcatg
    20341 atcaactgat gaggatggaa gccttgctgc tggcctttct cccataacgc cagacgctga
    20401 tgcacgccaa aacgatgctg ttcgtcgata atcaccagcg ccaggccgtt aaactgcacc
    20461 tgttcctgga agatggcgtg tgttccgaca atcatctgca cctgaccgct ggcgatggct
    20521 tcctgctgtg ccagtcgcgc tttacctttc tgcttaccgg cgagccagcc cacttcgata
    20581 ccgagcggtg caaaccagtt gcggaagtta ttcgcgtgct gttcggcgag taattcggtt
    20641 ggtgccatca atgccacctg tttgccgtgc gcaatcgcac gcagcgcggc gagggcagcg
    20701 accagcgttt taccggaacc tacatcgccc tgtaccaggc gcatcatcgg cgcatccagc
    20761 gccatatcgc gctcgatctc cgccaccacg cgtgcctgtg cgcccgttgg cttgaacggt
    20821 aaggctgcga ggagtttatt tttcagcgcg tcattggcgc taagcggctg ggcatggaaa
    20881 cgttgtgcac cagcgcgtaa cgccaacatg ctgaggttgt gcgcaagcag ttcttccaga
    20941 atcagacggc gttgtgctgg atgctgcccg gtttcgagat cgctaagctg tagcgtcggc
    21001 ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg tgacagttcc
    21061 ggcggcagga gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg atcggttaat
    21121 ttacgcagcg tggcctgctt tacgccttcc gttgttgggt aaaccggcgt tagcgtttcc
    21181 tgcaactctg gcgtactgag atcgccctgc acgcggtatt ccgggtggat catctccgca
    21241 ccatatttac cgcgctttgc ttcgccatag gccagcacgc ggcggcctgt cgccaggcta
    21301 tttttcattg ccgcgttgaa gttgaaaaag cgcatggtga gaatgccgga accgtcgctg
    21361 atctggcagg tcatcatccg ccgaccgccg aaggagatat tgcagttcag cacttcgcct
    21421 tccaccgtgg cataaacgcc cggtagtagt tctccgatgg ggtagagatg ggtgcgatct
    21481 tcgtagcgca ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt gattttcgcc
    21541 agcttgttac taagcgctgc gccaacgccc gttagggaac tgagcgggac agcatctaac
    21601 aggcgacctt tcatggcact taccctgcag cctgcatggt agcccaccag tcagcatcgg
    21661 cttcaatttc gccttgctga ttaacgtggg ggtaaggcag gcctttgcgt tttgcgactt
    21721 tcgccagcac cggatagccg ccttcaaaca gcaggcgttg ttgctctgct tccggcaaca
    21781 tgctgttttc acgcaggtac atgcctgcat tttgccgctg gcgctgggct tcataaagaa
    21841 tgagggctga ggcaacggaa acattcagcg actgcaccat gccgatcatc ggtatgatga
    21901 tgtcccgatc cgccagggcc aacgcttcct gcgtgatgcc tgttttctcc tgtcccatca
    21961 aaatacaggt cgggcgagtg taatcaattt cgcggaaatc gacagcgtta tcagaaagat
    22021 gggttgccag aatctgcatg ccctggcctt tcagatgagc gacggcatcg ccaatggtgc
    22081 ggtgcgtttt cacctgtacc cagctgttac tacccgcagc tgccgaagcc atggtgcgca
    22141 tccggctacc aggccagaca gcatgaactt catgtacacc aacggcatct gcggtacgaa
    22201 taatcgcaga aacgttatga ggtttgtgga cctgctccat gcagacggtc agatcaggct
    22261 gccgcctggc gagcatttcg cagatgcgtg cataacgtgt tgggttcata aaacattaat
    22321 ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg gattttgcgc atgatattcg
    22381 ccagatgcac acggtcacgg gcggtcagac gaataaaggc gctgtagacg cgaccatctt
    22441 tctcttccgt attcaaactt tgaatattcg aggtggtggt gttaattgcc gctgtcaggt
    22501 ttgccagcgc accctgatga ttgaacatct ccaccttgat ttcggtgatg aactcctgcg
    22561 ccgtctcttt atcccattcc acagccataa acttctctgg ctctttctgg tagccacgga
    22621 tgttacggca ggattcatgg tggatcacca gacctttacc ggggctgacg tgggcgataa
    22681 tcgggtcgcc aggaataggg cggcagcatt tcgcaaaggt gatcagcacg ccatcggcac
    22741 ctttaatggg cagatgtccg tgactttggg ttgccggtgg aatggaggcg tccccatgtt
    22801 gcagattttt cgcgaccacc acgctcattg cgttaccaag gccgatttca gccagcagat
    22861 cgtcaagcgt tgccagcttc atgcggtcca gctcgcgctg aatattttcc tgcgggattt
    22921 cattgagctt gcggctgcca cccaacgcat ggttgagcag acgacggccc aggcttacag
    22981 aatcatcacg cttgaggttt ttcagcaatt gacgaatttt ggcgcgcgct ttcgagctaa
    23041 cgacaaagtt cagccacgcg gcattcgggc gagcgcccgg tgcggtaata atttcaacgg
    23101 tttgaccgct ggtaagcggc tgcgacagcg ggtaaggctg gcggtcgacg cgtgcgccca
    23161 cgcaggcatg accgatatcg gtatgcaccg cataagcgaa gtcgacgggc gttgcaccgg
    23221 caggcagttc gacaatgcgc ccttctggtg tgaaaacgta aatctcatcc gggaagagat
    23281 cggatttaac gctctcgata aattcaaacg agctaccggc actctgttgc agctccagca
    23341 ggctttgcat ccagcgctgg gcgcgtattt gcgcggtagt gctggtttcg ccgtgctctt
    23401 tataagccca gtgcgcggca acacccatct ccgccatctg atccatatct tcggtacgga
    23461 tctggacctc aaccggcacg ccgtgcgggc caatcatcga ggtgtgcaaa gactgatagc
    23521 cgttcgcttt tggaatggcg atatagtctt tcacgcggcc cggacgcggc ttgtacaggc
    23581 tgtgcatctg gcccagcacg cgataacagg tgtcagaatc attgacgatc acgcggaaag
    23641 cgtagatgtc catgattgag tgaaaacgct gctctttgag caccattttg cagtaaatcg
    23701 aataaagatg cttttcgcga ccactgacgc ggcacggtat tcccgcttcc tgcaaacgcc
    23761 cttcgatttc agaaagaatc ttctggatca tctctttacg gttgccgcgc gcggctttca
    23821 ccacttcttt gattacgcga taacggttgg gatacagcgc ctcaaaaccc agctcttcga
    23881 gttcggtttt aatgtggtgg atacctaaac ggtgcgccag cgggctgtaa atttcgaggg
    23941 tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt
    24001 gggtacggtc ggcaagtttg atgagaatga cgcggatatc ctgcaccatc gccataatca
    24061 tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg gaacttgagc ttatcaagtt
    24121 tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc atgtcctggt
    24181 aggtggcggg agtatcttca atcacgtcat gcagcagcgc cgccataagc gtttcatagt
    24241 cgagtttcat ctcggccaga atgcaggcaa ccgccaccgg gtgcgtgata tagggttcac
    24301 cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac
    24361 gcttgatttg gtcttccggc aggtaggttt gaatcagttg attcaggctt tcaaacagat
    24421 acaagggcga cccgctttgt gattaacgac gaccttcagc aatagcggta acggcttgta
    24481 attcagcggc ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt
    24541 tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg ttttcttccg
    24601 gtaccagcgg atcctttccg cctacctgca tctgacgagc gcgacgcgcg gcgaccagta
    24661 ccaggtcaaa acggttacca attttctcta cagcgtcctg aacagttacg cgtgccatac
    24721 ttaaaaagct ccacaggtga agaaatgact gggcatgata ctgaaatcag gttcagtctg
    24781 ccaacaattt gctgattaaa gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt
    24841 cggcgcgaat aatggtcttc aaatcggtca acgcggtatc gaagtcatca ttcacaatca
    24901 ggtaatcata ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata cgctttgcaa
    24961 tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg gtccagttca attttggacg
    25021 gcggtaaaat aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcaccct
    25081 gccagtcgat atcgagaaaa acatcgacac cggtcgccag tacttgctca atggcctcac
    25141 gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa
    25201 tcatttcttt aaattcatca tgattaacaa agaaataatg ttcaccgtgg acctcaccag
    25261 gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac ggttgggttt
    25321 ttaataaagc ctgaatcagg ctggatttac ccgcgccact gggggcagaa acaatataaa
    25381 gcgtgccttg agccatgagt atctttcgta tgtgattagc gaaataaagc ctacatacga
    25441 gcttattata cacggcgcgg ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt
    25501 tgttgcgttt tgcgtagctg cttccgcatt tacctgtcat tcactgcaat aaagacgatt
    25561 ctcaggaagc gtcctcgcag actccgtttt tgccactcgg ctttgctttc atcacgttgt
    25621 atatctgcct gactatctac agcggaggtg atgatgaaag tatggatggc gatattaata
    25681 agtatcttgt gctggcaatc atcggcgtgg gcagtctgtc cggcctggtc gccagccaga
    25741 gcacaggaag aaatttcccg cctgcaacag caaataaaac agtgggacga tgactactgg
    25801 aaagaaggga aaagtgaggt ggaagacggt atttacgatc agttaagtgc ccgtctcacg
    25861 cagtggcaac gctgttttgg tgaagagacg catcgcgacg ctatgatgcc gcctttaaac
    25921 ggtgcggtta tacatccggt tgcacatacg ggtgtacgca aaatggcgga taaaatcgca
    25981 ttaagtctgt ggatgcgcga acgtagcgat ctttgggtgc agccaaaagt tgatggcgtt
    26041 gcggtaaccc tggtttatcg ggacgggaaa ctgaacaaag caatcagtcg cggcaatggc
    26101 ctgaaaggcg aggactggac gcagaaagtt cgcttaattt ccgctgtgcc gcaaaccgtt
    26161 agcgggcctt tagccaacag tacgcttcag ggggaaatat ttctcaaacg cgaggggcat
    26221 atccaaaaac aaatgggggg aataaatgcc cgcgcaaaag ttgctggtct gatgatgcgc
    26281 caggacgata gcgacacgct gaattctctg ggcgtttttg tctgggcatg gccggatgga
    26341 ccgcagttaa tgaccgatcg tttaaaagag ctggctaccg ctggttttac tctaacgcag
    26401 acgtataccc gtgcggtgaa aaatgctgat gaagttgcgc gcgtacgcaa tgcgtggtgg
    26461 aaaacgaaat tacccttcgt caccgatggc gtggttgtgc gagcggcgaa agaacccgaa
    26521 tcccgccatt ggctaccagg ccaggcagaa tggctggtgg cctggaaata tcaacctgta
    26581 gctcgggttg ccgaagtgaa ggcgattcag tttgcggtgg gtaagagcgg taaaatatcg
    26641 gtggttgcgt cactcgcacc tgtcatgctt gatgataaaa aagtccagcg ggtgaatatt
    26701 ggttccgtca ggcgctggca ggagtgggat attgcgcctg gtgatcagat cctcgtcagc
    26761 cttgccggtc agggtattcc tcgcattgat gatgtggtgt ggcgcggtgc agaacgtaca
    26821 aaaccgacac cgccagaaaa ccgctttaac tcgttgacct gctactttgc ttctgatgtt
    26881 tgtcaggaac agttcatttc acgcttagtc tggctggggt caaaacaggt tcttgggctg
    26941 gatggcattg gtgaggccgg ctggcgctcg ctgcatcaga ctcatcgctt tgagcatatc
    27001 ttttcctggc ttttattaac gccagagcaa ttacagaaca cgccggggat cgcgaaaagt
    27061 aaaagtgcgc agctatggca tcagtttaat ctggctcgta agcagccttt tactcgctgg
    27121 gtgatggcaa tgggaatacc gctaacccgg gcggcgctta atgccagtga tgaacggtcc
    27181 tggtcgcaac ttttattgag cacggagcag ttctggcagc agctgccggg gactggatcg
    27241 gggcgtgtca gacaggttat tgaatggaag gaaaatgcgc aaatcaagaa gctgggcagt
    27301 tggctggctg cccagcagat cacaggtttt gaaccttagt gtccttcgtg gctgtagtaa
    27361 aagaccggta atcccagctt aaggcgtagc gccagtaagc gggcaaaaaa gccgaatacc
    27421 agggtggaga taattaccac atcatggtta gaaacatagt gttgcagcgc gatgtacagc
    27481 acggcggagg caaatgagac accggcatac aactctttct ggaataccag cgggatgcgt
    27541 ttacagaaca tatcgcgcaa aacgccgcca aatacgccgg tcgtcacagc tgcgacaacg
    27601 gcaatgatcg ggccgtgtcc catatccagt gcaacctgtg cgccaatgat agaaaagacc
    27661 accagtccga gcgcatcgag caccagaaac actttgcgca ggtaaggcat tacaggagca
    27721 acgatagtgg tgagtactgc ggcggtagcg acaataatca catattccgg gtgtttgacc
    27781 cagccgagcg gatagtggcc cagcagaata tcgcgcactg accctccgcc aattgccgtg
    27841 gcggtcgcaa taataattac gccaaatgta tccatgcgcc gtcgtccggc cgccagcgcc
    27901 cccgtcatgg cttcggcagt aatgccaacc aaatacaaaa tgtgtaacag catggttctc
    27961 tccctaaaaa tctgtctggc agggtagcga tttgtgcgta ttgtcacgat tgagattttt
    28021 taaggtgaga ggaaattgat aacttgtagt tatgtgaaat cgctattttc tgtagcgtag
    28081 atgcattctt ctgacttctt gtctgagaaa atgcataaga aaaaataggt taaaattgag
    28141 gggtggaaaa gaaaggtgca atgtttagcc attgtgttca ccgcacctgg actttggctt
    28201 acgcatcggg tatctcaatc aacaaaaaga gtttttagtt tctgctattt cttctctgta
    28261 atttttacac tgttttccaa ctgcttgatg ttgacctgcg gggtcggcaa ctcctcaggc
    28321 atggttccgc caagttcctg aatggtttgc ctcaccttgc gacccacgtc aaagtgtgtg
    28381 gtgttagctt gctgttttga attcacgcca tcccgcttga gtttttcttc tgtttgggta
    28441 gctcgaaaga gattagccgc cagttctgtt gaacccatat gatccaggat cttctgactc
    28501 tttttcagcc ccttccgctg atggatggct ttctgatcta atccaccata cagcccccgg
    28561 taaccatgat tctgaaatat ggcgaaatcg atagctgtcg ctaccccagc ttgctgcgct
    28621 gcctcaacta attgtttgtt atgttctttc aactcatttc tcagaaaaag acgcttttcg
    28681 tcttcacgaa gttgcctgaa agcttcatcg tcagccagct cctgccgtcg ggtctggata
    28741 gcaaaataag tttgccctgc cgcaataacc ggtttcgcag ggtcgccgtt ttgtactacc
    28801 aggtaacagg catagcggga gagtacgatg tcttttaatg ctcttttggc atttgagcca
    28861 attttgacca tatcgaggat ctcctcgaaa tggtcatcaa ttgtgtgacc actattcaga
    28921 caagcttctt tggcgcgcgt taatacaggt ataaagtgac gatactctga ataacccagt
    28981 agttttccaa gttgccttgc tgaccattgt tctgctccgt ttgcattaat cagctttatc
    29041 tcttcaaaag gttgatgatg ttcgttcatt gcccactcca tgtgatatcg attgcaagga
    29101 aagtaagtct acaactgtgt ttatatacag ttgtaagtat ctcacgttct cactgcgctg
    29161 ggcaaaaaaa catcgcaaaa tctggagcag gcacgcaaaa cagaattgaa tgttgcgggg
    29221 aggaaaaatg ccgctctgtt taagtgggcg gcatttgtga aggctgaaaa ggaagaattg
    29281 gcgttacgag ttattcaatg ttctggatct gctcgcgcat ctgctcaatc aacactttca
    29341 gctcgatggc agaattcgtc acttcggcat tgatagattt cgacgcaaga gtgttcgact
    29401 cgcggttgaa ctcctgcatc ataaagtcca gacgacgacc aaccgcttct tttttcttca
    29461 gaatgttgta ggtctctttg acatgcgctt cgaggcggtc cagttcttcg gcaacgtcaa
    29521 ttcgttgtgc cagcagaacc aactcctgct ccagacggtt gttttccagt tgcacctgag
    29581 catcttccag cttcgcgacc agacgctcac gctgccattg caggatttcc ggcatatggg
    29641 cgcggacttt gaccacttcg gcggtgacgc cttccagacg ctgctcgatc aatgctttca
    29701 gcgcctgacc ttcggtttcg cgcgcgacga taaagtcgtc cagcgtacca tccaacgccg
    29761 cgagaatttc agcggcaatc gcgtcgagat cctgctcctg ggctgccatc acgcccggcc
    29821 agcgtagaat atcaaccggg ttgatttccc cttcgtcact ctgcattttt acccagttcg
    29881 cggcagttac cagctgttta gccagttttt cgttgaggat cagctcacct tgcgcgctaa
    29941 catctggctc atagcgcagg gtacattcca ctttaccgcg cgtcaggcga gaacgaatac
    30001 gctcacgaac gacaggttca aggctacgga actgctccgg cagacgaaag taagtttcca
    30061 gataacgctg gtttaccgag cgcatttccc aggttgcgct cccccattca cccttgattt
    30121 cacgccgggc gtaggcggtc atactgcgga tcatagacgt tcctgtttat aaaaggagag
    30181 gtggaaggat tatagccatc gatgccttgt caggatagga ataaccgccg gaagtccgta
    30241 taatgcgcag ccacatttgt ttcaagccgg agatttcaat atgcgtccag caggccgtag
    30301 caataatcag gtgcgtcccg ttaccctgac tcgtaactat acaaaacatg cagaaggctc
    30361 ggtgctggtc gaatttggcg ataccaaagt attgtgtacc gcctctattg aagaaggcgt
    30421 gccgcgcttc ctgaaaggtc agggccaggg ctggatcacc gcagagtacg gcatgctgcc
    30481 acgttctacc cacacccgta acgcgcgtga agcggcgaaa ggtaagcagg gtggacgcac
    30541 catggaaatc cagcgtctga tcgcccgtgc tcttcgtgcg gcagtagatt tgaaagcgct
    30601 gggtgagttc accattacgc tggactgcga cgtgcttcag gctgatggtg gcactcgtac
    30661 cgcgtcgatt acgggtgcct gcgtggcgct ggcagatgcg ctacagaagc tggtggaaaa
    30721 cggaaagctg aaaaccaacc cgatgaaagg gatggtagcc gcagtttctg tcggaattgt
    30781 gaacggcgaa gcggtttgcg atctggaata cgttgaagac tcagccgcag agaccgacat
    30841 gaacgtagtg atgaccgaag acgggcgcat cattgaagtg caggggacgg cagaaggcga
    30901 accgttcacc cacgaagagt tactcacctt gttggcgctg gcccgagggg gaatcgaatc
    30961 cattgtagcg acgcagaagg cggcgctggc aaactgattt ttaaggcgac tgatgagtcg
    31021 cctttttttt gtctgtagaa aagtaagatg aggagcgaag gcatgaaacc atatcagcgc
    31081 cagtttattg aatttgcgct tagcaagcag gtgttaaagt ttggcgagtt tacgctgaaa
    31141 tccgggcgca aaagccccta tttcttcaac gccgggctgt ttaataccgg gcgcgatctg
    31201 gcactgttag gccgttttta cgctgaagca ttggtggatt ccggcattga gttcgatctg
    31261 ctgtttggcc ctgcttacaa agggatcccg attgccacca caactgccgt ggcgctggcg
    31321 gagcatcatg acctggacct gccgtactgc tttaaccgca aagaagcaaa agaccatggt
    31381 gaaggtggca atctggtcgg tagcgcgtta caaggacgcg taatgctggt ggatgatgtg
    31441 atcaccgccg gaacggcgat tcgcgagtcg atggaaatta ttcaggccaa tggcgcgacg
    31501 ctggctggcg tattgatttc gcttgatcgt caggaacgcg ggcgcggcga gatttcggcg
    31561 attcaggaag ttgagcgtga ttacaactgc aaggtgatct ctatcatcac cctgaaagac
    31621 ctgattgctt acctggaaga gaagccagaa atggcggaac atctggcggc ggttaaggcc
    31681 tatcgcgaag agtttggcgt ttaaagaaac tcgccggatg aaaagtcatc cggcgtcata
    31741 ttactgcaac tgtgcggcaa ttagcggcca gcgggcgtca aaatcatccg tcgggcggta
    31801 tttaaactcg ctgcggacaa agcgtgacag cataccttca cagaaggcca gcaactggct
    31861 tgccagcagg gtttcatcgg tggtgtaacc ttcaccctca cgcattctct tttcacgcaa
    31921 tacctgacgc aactgcgctt caatacgctc gaacagctgg ttgatgcgcc cttgcagacg
    31981 atcctgttcg aacattagcg catgaccagt gaggatgcga gtcaggccag gattacgctc
    32041 accaaaaccg agaagcagca acacaatcag acgcaggcgc gctgtggtgt ccttctcatc
    32101 tttcagaatc agattgatgc gagtaatcag gctatcttcg ataaactcaa tcaggctatc
    32161 gaacatgcgg gtcttactgg ggaagtggcg atacagtgcc gcttcggaaa cgccgacaga
    32221 ggcggccagt tttgccgtcg tgatacgttg gcttccatcg ctggattcca gcatcagcgc
    32281 cagagactga agtatttcct cgcgacggtt ccttttcgca gtttgttttt ctgccatgtt
    32341 acaaaatacc cctgaaaata agcacttgcc aggcggcacc cacgctatga ccgcaaacga
    32401 aatgtttgcg gctatgttat gacgttattc ggatgcgtat gtgttactga cgaccagagt
    32461 gaccaaagcc gccttcaccg cggtcggtgg cgtcgaaatc ttccaccaga ttaaattcag
    32521 cctgtactac cggaacaaaa atcatctggg cgatgcgttc gccaggttga atggtgaagc
    32581 tgtcctgacc acggttccac acggaaatca tcaactggcc ctgatagtca gaatcgatca
    32641 atcctaccag gttaccaagc acgataccgt gcttatgtcc caatccggag cgcggtagca
    32701 tcattgccgc cagtgaagga tcggcaatat gaatcgccag cccggtcgga accagcgtag
    32761 tgtcacccgg agccagttct acggcgtcgt cgagacaggc acgcaggtca agtccggcag
    32821 agccagaggt ggcataagtc gggagcggaa attccttccc aacgcgcggg tccagaatct
    32881 taacgtcgat ttttttcatc ataacgggtc acgatctcgt cgagtaataa ttggccaagg
    32941 agctctttgc gctcaagcgg taagacttta tctccgtcct gccagaaaag gtgtaatgcg
    33001 ttgttgtcgc tgttaaatcc ttgagttggc tgggaaacat cgttcgcgca gatcagatca
    33061 aggtttttac ggatacgttt ttgccgggcg tattcttcca cattatttgt ttcggcggca
    33121 aatccaacga cgtaggggcg atggtctttt agtgcggcaa cgcctgcgac gatatcgggg
    33181 tttttaacca tttttattgt taattcatca ccctgcgtgg cctgcttttt gattttttct
    33241 ggggccacgg tagctgcgcg ataatccgcc acggcggcgc aaccgataaa aatattttgc
    33301 tgctgtacag atgcattcac ggcggcttcc atttccagcg cggtcatcac atcaacacgt
    33361 ttaacaaacg gtggcgtcgg tagtgaaacc ggacctgata ccagcgtgac gttcgcgcca
    33421 cgacgggcag cggcggcggc gatagcaaaa cccatcttgc ctgagctgtg attagagata
    33481 taacgcaccg gatcgagtgg ttcacgcgtc gggccggcgg taatcataat gttcagatgt
    33541 ttcaggtcgt tgacgggcga aaaatgcgct accgccatat caacaatggt taacggatcg
    33601 agcattcgcc cagggccgat atcaccacaa gcctgactgc cactgtctgg cccccaaatg
    33661 agcaaaccac gggaagcaag cacctctaaa ttatgctgcg tggcagcggc acggtacatc
    33721 tgctggttca tggcggggag cacggctaca ggcgcaggtg tggccagaca aatcgtcgat
    33781 accagatcat tcgccattcc ggcagcaaca cgggcaatca aatctgccgt ggcaggggcg
    33841 agaatcacta aatcagccca tttacccagc tcaatatggc ccatagcggc ttcggctgct
    33901 gggtccagca gactgtcgga aacgggataa ccagaaaccg cctgcaagct aagtggggtg
    33961 ataaaggctt ttgccgcttc ggtcatggct acgcggacgt cggccccgcg ctcgcgcaaa
    34021 cgacgcacca gttcaggggt tttataggca gcaataccgc cgctgacgcc gagaacgatt
    34081 tttttaccgg ccaggctcat gatgattttt cctgttgggt gacaccagaa gttggcgatt
    34141 ttatcacaat acttttgttg tcgtgccttc acctgagaat cactttgcga ggcgctttcc
    34201 aggattgaaa actggccgtc gatttaacgg aacggctatg acaggatgcg agcaccacaa
    34261 aggaggtgaa ggtgaaaaac aattcacagc tgttgatgcc gcgcgaaaaa atgctgaagt
    34321 ttggtattag cgccttaacg gatgtcgagc tgctggcgct atttctgcgt accggaacgc
    34381 gcggtaaaga tgtattaacc ctggcaaaag agatgctgga gaatttcggc tctctttatg
    34441 gcttgttaac ctctgaatat gaacaattta gtggtgttca tggaattggc gtggcgaaat
    34501 ttgcccagtt aaaggggatt gccgaactgg cgcggcgtta ctacaacgtg cggatgcgtg
    34561 aagaaagccc tttactcagc ccggagatga cgcgggaatt tttacaaagc cagctcacgg
    34621 gtgaggagcg ggagatcttt atggtgatct ttctcgactc acaacacagg gttataacgc
    34681 atagccgtct tttttccggc acgctaaacc atgttgaagt ccatcctcgg gaaattatcc
    34741 gcgaagcgat aaaaataaat gcctcggcgc tgatccttgc acataatcac ccttcgggtt
    34801 gtgctgaacc cagtaaagcg gataaactca ttactgaacg gataataaag agttgtcagt
    34861 tcatggattt acgcgtgctc gaccatatcg tgattgggcg tggagagtat gtttcttttg
    34921 ccgaacgcgg ctggatttaa cccgctatgc gcgatccttc gggatctttg tctgttcggg
    34981 acttgagcac atcgctgagt cagcgtatac tacgccacct ttgagaatct cgggtttggc
    35041 atttgggcct ggcaatcgag agttcacata gaactgcgat gaccgggctg taaagcctga
    35101 cgaggcgcca ataccccata cgaagctcga gctaatttga tttttggaga atagacatgt
    35161 cccgagtctg ccaagttact ggcaagcgtc cggtgaccgg taacaaccgt tcccacgcac
    35221 tgaacgcgac taaacgccgt ttcctgccga acctgcactc tcaccgtttc tgggttgaga
    35281 gcgagaagcg ttttgtcacc ctgcgcgtat ctgctaaagg tatgcgtgta atcgataaaa
    35341 aaggcatcga tacagttctg gctgaactgc gtgcccgtgg cgaaaagtac taagtactta
    35401 gaggaaataa atcatggcta aaggtattcg tgagaaaatc aagctggttt cttctgctgg
    35461 tactggtcac ttctatacca ctacgaagaa caaacgtact aagccggaaa aactggaact
    35521 gaaaaaattc gatccagttg ttcgccagca cgtgatctac aaagaagcga aaatcaaata
    35581 atttttgctt tgatgtaacg aaaaacctcg ccccggcggg gttttttgtt atctgcctgc
    35641 ccccatattg actgcatctg ttcattcctg gagatgctat gcctgaatta cccgaagttg
    35701 aaaccagccg tcgcggcata gaaccgcatc tcgttggtgc aaccattctt catgcggtgg
    35761 tgcgcaacgg acgcttgcgc tggccggttt cagaagagat ctaccgttta agcgaccaac
    35821 cagtgcttag cgtgcagcgg cgggctaaat atctgctgct ggagctgcct gagggctgga
    35881 ttatcattca tttggggatg tctggcagcc tgcgcatcct tccagaagaa cttccccctg
    35941 aaaagcatga ccatgttgat ttggtgatga gcaacggtaa agtgctgcgc tacaccgatc
    36001 cgcgccgctt tggtgcctgg ctgtggacca aagagctgga agggcacaac gtgctggccc
    36061 atcttggacc ggagccgctt agcgacgatt tcaacggtga gtacctgcat cagaagtgcg
    36121 agaagaaaaa aacggcgatt aaaccatggc tgatggataa caagctggtg gtaggcgtag
    36181 ggaatatcta tgccagcgaa tctctgtttg cggcggggat ccatccggat cggctggcgt
    36241 catcactgtc gctggcagag tgtgaattgt tagctcgggt gattaaagcg gtgttgctgc
    36301 gttcgattga gcagggcggt acaacgctga aagattttct gcaaagtgat ggtaaaccgg
    36361 gctatttcgc tcaggaattg caggtttacg ggcgaaaagg tgagtcgtgt cgggtgtgtg
    36421 gtacgccgat tgtggcgact aaacatgcgc agcgggcaac gttttattgt cggcagtgcc
    36481 agaagtaatt gatgcgcgcc ggatggcata ccatccggca taaactctac gctaacttcg
    36541 ccatcagcgc ctgatggaca ttctccggca ggaaatgggt gacatcgccc tgatggcgcg
    36601 ccacctcttt caccaacgat gaagagataa acgaccactc tttcgacggc atcagaaaca
    36661 cactttccag ttccggcatt aagtggcgat tcatatgcgc cagctgcatt tcatattcaa
    36721 aatccgccac cgcacgcagg ccacgaatca gcaccgtagc gtgttgatta cgggcgaagt
    36781 tcgccatcaa atcactaaac ccgaccactt ccacgttccc cagatgcgcg gttgcctgct
    36841 gtgccagcgc cacacgctct tccagagtaa acatcggttt tttactggga ctggcggcga
    36901 tcgccagaat aacgtgatcg aacatttgcg tggcgcgcgt cacgatatcg atatgaccat
    36961 tggtaatggg atcgaaagta cccggataaa tcgcccgttt ttgcataaca acctcaatgc
    37021 gttttcggtg gcaggtaagg ttccagcagt tgaagcagac gctgtagcgc gccctggttt
    37081 tgatacagta cttcaacggc atgacggcca tagaaactac ggtaatcggc gtcggtgagt
    37141 aaagaggaaa cctcttttgc aagcgacgtg gcatcggtaa cggtaatcag cccgcttgcc
    37201 tgctccagcc gcgcgcaaat gtctttaaag ttaaaagtat gaggccccat caataccgga
    37261 atagcgtgtg cggcagcttc cagcggatta tgcccaccac gttcaaccag tgaaccgcca
    37321 acaaaggcga gatcggcaat gccatacagt aacatcaact cgcccatcgt atcgccaacc
    37381 acaacctgcg tgctggtgga ggggacttcc cctgaagagc gtgtgatata gcttagtcca
    37441 gcctggcgga caaggttaat cgcatccggg aagcgttccg gatgacgggg gaccaggatg
    37501 agcaataaat tcgggaattg ctgtaacaat gcctgatgtg cggcgatcac cacactctct
    37561 tcgccttcgt gagtgctggt ggcaatccat accgggcggt gtggtgccca ctggcggcgc
    37621 agcgtcacgg ctttagcagc caactgcggc gttacagaaa tatcgaattt caggctaccg
    37681 gtaacggtta cctggttatt ttttgcgccc agcgccacaa aacgtgcacc atcttcttca
    37741 ttttgcgcag caatcagcgt aatacgacgc agcaagcgac ggacgaattt acccagtttg
    37801 gcataacctg cggccgagcg ggcagagagt cgcgcgttag cgatcaccag cggaatttta
    37861 cgtttatgta gcgccgcaat caggttaggc catagttcgg tttccataat caacactagt
    37921 ttagggtcga ctttattcag gaaacgatta agtgcatcgg gcaggtcata tggcagataa
    37981 acgtgctgaa catccttccc gaaagccgat tgtacgcgct ccgaaccggt tggcgtcatg
    38041 gttgtaacgg taatcggtaa atcaggataa cgatggcgca gcgcgcgcac caacgggatc
    38101 gccgccagag tttcaccgac ggagacggag tgcagcatga tgccgcctgg ttttagcgga
    38161 tggcggtaaa aaccgtaacg ttcaccccag cgttttcgat aggccggagc cttacgtccg
    38221 cgcacccaga gccgtatcca gatcagcggc tgaataaggt agagaagggc ggtgtaaagc
    38281 aattcgagca tagtaaatag ctgacttatg gatgtgctgg ggattctatg tatttagctg
    38341 tggctttacc attacttttc ccgtttttga cttaaatagc aatttacccg ccaattcttg
    38401 ctgaacatct atactttttt tgtattttta tgcgattcat tgatttctgc agtacgtgca
    38461 ggaaggaatc gcagagcaac agtgaaagaa tatggttgct aatgatttta tttgattgat
    38521 ttctttattg aaaatcaaca atgtgcttaa aaatcattcg ggtcggattg ccgcgaaaga
    38581 agtgatacac tagcgcgtca atatcagtgc ggtatcagta ttcaggtagc tgttgagcct
    38641 ggggcggtag cgtgcttttt ctgcttcacc ttaatcggat aatctcaaca aaaagagtta
    38701 cttgtggata agccatttcg aagaattttg ctcattaaga tgcgttttca tggggatatg
    38761 ttattaacta ctcccgtcat tagttcgctg aaaaaaaatt accctgacgc aaaaatcgat
    38821 gtgctgcttt atcaggacac catcccgatc ctgtctgaaa atccagagat taacgcgctc
    38881 tacggcataa aaaataaaaa agcaaaagcc tcagaaaaaa ttgccaactt tttccatctc
    38941 atcaaggtat tacgtgccaa taagtatgac cttatcgtta atcttaccga tcaatggatg
    39001 gttgctatac tggtgcgctt attaaatgcc cgtgtgaaaa tttcccagga ttatcatcat
    39061 cggcagtctg ctttttggcg taaaagtttc acccatttgg tgccgttgca gggtggaaat
    39121 gtggtggaaa gtaacttatc cgtgctgaca ccactgggag ttgattcgtt ggtgaagcag
    39181 acaaccatga gttacccgcc tgcaagctgg aaacgtatgc gtcgcgaact tgatcacgct
    39241 ggtgttggac aaaattatgt ggttatccaa cctacggcgc ggcaaatctt caaatgctgg
    39301 gacaacgcca agttttccgc tgtgattgat gccttacatg ctcgtggtta tgaagtcgtt
    39361 ctgacgtccg gcccagataa agacgatctg gcctgcgtca atgaaattgc gcagggatgc
    39421 cagacgccac cagtaacggc gctggccgga aaggtgacct tcccggaact tggtgcgtta
    39481 atcgatcatg cgcagctgtt tattggcgtt gactccgcac cggcgcatat tgccgctgca
    39541 gttaatacgc cgctgatatc gctgtttggt gcgacagacc atattttctg gcgtccctgg
    39601 tcaaataaca tgattcaatt ctgggcggga gattaccggg aaatgccaac gcgcgatcag
    39661 cgtgaccgaa atgagatgta tctttcggtt attccggcgg cagatgtcat tgctgctgtc
    39721 gataaattac tgccctcctc cacgacaggt acgtcgttat gatcgttgct ttttgtttat
    39781 ataaatattt tccctttggc ggtttgcagc gcgattttat gcgtattgct cagacagtcg
    39841 ccgcccgagg tcatcatgtt cgggtttata cccagtcgtg ggaaggcgaa tgccctgatg
    39901 tatttgaact gatcaaagtg ccggttaaat cgcataccaa tcacgggcgc aatgcggagt
    39961 attttgcctg ggtgcaaaaa catttacgcg aacatcccgt cgataaagtc gttggattca
    40021 acaaaatgcc ggggctggac gtttattatg ccgctgatgt ttgttatgcc gagaaagtag
    40081 cgcaggaaaa aggctttttc tatcgcctga cgtcacgtta tcgccattat gccgcctttg
    40141 agcgggcaac cttcgaacag ggcaagccga cacagctgct gatgctgaca gataagcaaa
    40201 tcgccgattt ccagaaacat tatcagactg aagcggagcg ttttcatatt ctgccaccgg
    40261 ggatttatcc tgatcgtaaa tatagccagc agccagctaa tagccgtgaa atcttccgta
    40321 agaagaatgg aataaccgaa caacaatatt tattgttgca ggtcggttca gacttcacgc
    40381 gtaaaggtgt cgatcgttcc attgaagcac ttgcttcgtt accggattcg ctgcgccaca
    40441 acacattgct atatgttgtt gggcaggata aaccgcgaaa atttgaggcg ctggcagaaa
    40501 aacgcggcgt gcgcagtaat gttcacttct tctcggggcg caacgatgtc tcggaattaa
    40561 tggcggcggc ggatttatta ctgcatcctg cctaccagga agcggcggga attgtgctgc
    40621 tggaagcgat tactgcagga ttaccggtac taacaactgc cgtttgtggc tatgcgcatt
    40681 atattgtcga cgccaattgc ggcgaggcta ttgctgagcc attccgccag gaaacattga
    40741 atgagatttt acgcaaagcg ttaacgcaat tttcattgcg ccaggcctgg gcggaaaatg
    40801 cgcgacatta tgctgataca caagatttat acagtctgcc agagaaagcg gcggatatca
    40861 taacgggtgg tctggatggt tgaacttaaa gagccgtttg ccacgttatg gcgcggaaaa
    40921 gatccttttg aggaagttaa aactttgcag ggtgaggtat ttcgtgaact ggaaactcgc
    40981 cgtactctgc gctttgaaat ggcgggcaaa agctattttc tcaaatggca tcgcggcacg
    41041 accctgaaag agataatcaa aaatttactc tcattgcgga tgccagtatt aggcgcagac
    41101 cgcgaatgga atgcgattca tcgactgcgg gatgtcggcg ttgatactat gtatggggtg
    41161 gcattcggcg aaaaaggcat taatccgctc accagaacct cgtttattat aaccgaagat
    41221 ctgacaccaa ccatcagtct ggaagattac tgtgctgact gggcgactaa ccctccagat
    41281 gttcgcgtaa agcgtatgct tattaagcgt gtcgcgacga tggtgcgcga tatgcatgct
    41341 gcgggcatta accaccgtga ctgttatatc tgtcatttcc tgctgcactt gcctttttcc
    41401 ggtaagaaag aggagttaaa aatttcggta attgatctgc accgggcgca gcttcgcacg
    41461 cgcgttccac gtcgttggcg cgataaagat cttatagggc tttatttttc ttcgatgaat
    41521 attggcctga ctcagcggga tatctggcgg tttatgaaag tgtattttgt cgcctcgctt
    41581 aaagacattc tcaagcagga acaaggactg ctgtcgcaag cagaagcaaa agccacaaaa
    41641 atcagggaaa gaacgattcg aaaatcgttg taatttccgg agagaaatat gagtgcccac
    41701 tattttaatc cacaagagat gatcaataag acaatcatct tcgatgaaag gccagcggcg
    41761 tcagtggcat catcattcca tgttgcttat ggcattgata aaaactttct ttttggttgt
    41821 ggtgtttcaa tcacgtcagt tttgttacat aacaacgacg taagttttgt tttccacgtt
    41881 tttattgatg atatccctga agccgatatc cagcgtttag cccaattggc gaaaagctat
    41941 cgtacctgta tccagatcca tctagtaaat tgtgaacggc ttaaggcatt accgacgacc
    42001 aaaaattggt ctattgccat gtatttccgt tttgtaattg cagattactt tattgatcaa
    42061 caagataaga tcctttacct ggatgctgat atcgcctgtc agggaaactt aaagccgctg
    42121 ataacaatgg atcttgccaa taacgttgct gctgttgtta ctgaacgcga tgctaactgg
    42181 tggtcgttac ggggtcaaag tctgcagtgt aatgaacttg aaaagggtta ctttaattca
    42241 ggtgtcctgt taattaatac actagcgtgg gcgcaggagt ccgtttctgc taaagcgatg
    42301 tcgatgcttg ctgataaagc catcgtttcc cgtttaacct atatggatca agatatcctt
    42361 aatcttatcc tgttagggaa agttaaactc attgaagcta aatacaatac gcaatttagt
    42421 ttaaattatg aattaaaaaa atcatttgtt tgtccaatta atgatgaaac cgtattaatt
    42481 cattatgtcg gcccgacaaa accctggcat tactgggccg gttatccaag tgcgcaacct
    42541 tttatcaaag ccaaagaagc atcgccctgg aaaaatgaac cgttaatgcg gccagttaac
    42601 tcaaactatg ctcgttattg cgctaagcat aattttaaac aaaacaaacc aattaacggg
    42661 ataatgaat
//
LOCUS       DAZGTJ010000032        41822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000032 DAZGTJ010000000
VERSION     DAZGTJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.032"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            107..3865
                     /gene="yfaL"
                     /locus_tag="ACHK8E_002196"
     CDS             107..3865
                     /gene="yfaL"
                     /locus_tag="ACHK8E_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP0344463.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQ
                     YAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     GVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4005..4727)
                     /gene="ubiG"
                     /locus_tag="ACHK8E_002197"
     CDS             complement(4005..4727)
                     /gene="ubiG"
                     /locus_tag="ACHK8E_002197"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP0344464.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4874..7501
                     /gene="gyrA"
                     /locus_tag="ACHK8E_002198"
     CDS             4874..7501
                     /gene="gyrA"
                     /locus_tag="ACHK8E_002198"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP0344465.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7650..9338
                     /gene="yfaA"
                     /locus_tag="ACHK8E_002199"
     CDS             7650..9338
                     /gene="yfaA"
                     /locus_tag="ACHK8E_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP0344466.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9335..9958
                     /gene="yfaT"
                     /locus_tag="ACHK8E_002200"
     CDS             9335..9958
                     /gene="yfaT"
                     /locus_tag="ACHK8E_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP0344467.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9982..14496
                     /locus_tag="ACHK8E_002201"
     CDS             9982..14496
                     /locus_tag="ACHK8E_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP0344468.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14497..16146
                     /gene="yfaQ"
                     /locus_tag="ACHK8E_002202"
     CDS             14497..16146
                     /gene="yfaQ"
                     /locus_tag="ACHK8E_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP0344469.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16151..16927
                     /gene="yfaP"
                     /locus_tag="ACHK8E_002203"
     CDS             16151..16927
                     /gene="yfaP"
                     /locus_tag="ACHK8E_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP0344470.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17001..18185)
                     /gene="atoB"
                     /locus_tag="ACHK8E_002204"
     CDS             complement(17001..18185)
                     /gene="atoB"
                     /locus_tag="ACHK8E_002204"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFP0344471.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18216..19538)
                     /gene="atoE"
                     /locus_tag="ACHK8E_002205"
     CDS             complement(18216..19538)
                     /gene="atoE"
                     /locus_tag="ACHK8E_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFP0344472.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19535..20185)
                     /gene="atoA"
                     /locus_tag="ACHK8E_002206"
     CDS             complement(19535..20185)
                     /gene="atoA"
                     /locus_tag="ACHK8E_002206"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFP0344473.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20185..20847)
                     /gene="atoD"
                     /locus_tag="ACHK8E_002207"
     CDS             complement(20185..20847)
                     /gene="atoD"
                     /locus_tag="ACHK8E_002207"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFP0344474.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21043..22428)
                     /gene="atoC"
                     /locus_tag="ACHK8E_002208"
     CDS             complement(21043..22428)
                     /gene="atoC"
                     /locus_tag="ACHK8E_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFP0344475.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22425..24251)
                     /gene="atoS"
                     /locus_tag="ACHK8E_002209"
     CDS             complement(22425..24251)
                     /gene="atoS"
                     /locus_tag="ACHK8E_002209"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFP0344476.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24418..27267
                     /gene="rcsC"
                     /locus_tag="ACHK8E_002210"
     CDS             24418..27267
                     /gene="rcsC"
                     /locus_tag="ACHK8E_002210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP0344477.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27353..28003)
                     /gene="rcsB"
                     /locus_tag="ACHK8E_002211"
     CDS             complement(27353..28003)
                     /gene="rcsB"
                     /locus_tag="ACHK8E_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP0344478.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28048..29337)
                     /locus_tag="ACHK8E_002212"
     CDS             complement(28048..29337)
                     /locus_tag="ACHK8E_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0344479.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(29371..29625)
                     /gene="xisR"
                     /locus_tag="ACHK8E_002213"
     CDS             complement(29371..29625)
                     /gene="xisR"
                     /locus_tag="ACHK8E_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0344480.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(29633..29779)
                     /locus_tag="ACHK8E_002214"
     CDS             complement(29633..29779)
                     /locus_tag="ACHK8E_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344481.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(29800..30042)
                     /locus_tag="ACHK8E_002215"
     CDS             complement(29800..30042)
                     /locus_tag="ACHK8E_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344482.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            complement(30035..30937)
                     /locus_tag="ACHK8E_002216"
     CDS             complement(30035..30937)
                     /locus_tag="ACHK8E_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344483.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(30934..31158)
                     /locus_tag="ACHK8E_002217"
     CDS             complement(30934..31158)
                     /locus_tag="ACHK8E_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344484.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(31175..31537)
                     /locus_tag="ACHK8E_002218"
     CDS             complement(31175..31537)
                     /locus_tag="ACHK8E_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP0344485.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(31530..32396)
                     /locus_tag="ACHK8E_002219"
     CDS             complement(31530..32396)
                     /locus_tag="ACHK8E_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP0344486.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(32420..33004)
                     /locus_tag="ACHK8E_002220"
     CDS             complement(32420..33004)
                     /locus_tag="ACHK8E_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344487.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(32991..33551)
                     /locus_tag="ACHK8E_002221"
     CDS             complement(32991..33551)
                     /locus_tag="ACHK8E_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344488.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(33959..34321)
                     /locus_tag="ACHK8E_002222"
     CDS             complement(33959..34321)
                     /locus_tag="ACHK8E_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344489.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGQKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            complement(34496..34696)
                     /locus_tag="ACHK8E_002223"
     CDS             complement(34496..34696)
                     /locus_tag="ACHK8E_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344490.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(34693..34884)
                     /locus_tag="ACHK8E_002224"
     CDS             complement(34693..34884)
                     /locus_tag="ACHK8E_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344491.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(34886..35263)
                     /locus_tag="ACHK8E_002225"
     CDS             complement(34886..35263)
                     /locus_tag="ACHK8E_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0344492.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(35349..36008)
                     /locus_tag="ACHK8E_002226"
     CDS             complement(35349..36008)
                     /locus_tag="ACHK8E_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0344493.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            36348..36641
                     /locus_tag="ACHK8E_002227"
     CDS             36348..36641
                     /locus_tag="ACHK8E_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344494.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            36887..37255
                     /locus_tag="ACHK8E_002228"
     CDS             36887..37255
                     /locus_tag="ACHK8E_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344495.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            37289..38947
                     /locus_tag="ACHK8E_002229"
     CDS             37289..38947
                     /locus_tag="ACHK8E_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0344496.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            38949..39917
                     /locus_tag="ACHK8E_002230"
     CDS             38949..39917
                     /locus_tag="ACHK8E_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP0344497.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            39917..40780
                     /locus_tag="ACHK8E_002231"
     CDS             39917..40780
                     /locus_tag="ACHK8E_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0344498.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            40859..41284
                     /locus_tag="ACHK8E_002232"
     CDS             40859..41284
                     /locus_tag="ACHK8E_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0344499.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            41484..41640
                     /gene="hokD"
                     /locus_tag="ACHK8E_002233"
                     /pseudo
     CDS             41484..41640
                     /gene="hokD"
                     /locus_tag="ACHK8E_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
BASE COUNT        10254 a        10021 c        10998 g        10549 t
ORIGIN      
        1 acaaaatagt tattacatca ggggcagaat ttacaaaaga aaaataccct atatcgcata
       61 atgcgattaa atacgccatc ttatagaaaa tgatggttag atgaatatgc ggattatctt
      121 tctacgcaag gagtatttat ctttactccc gtcaatgatt gcatctcttt tctctggtaa
      181 cggtgccgca gcggtcatcg attcatgcca gggatatgat atcaaagcga actgtcaggc
      241 cagcaggcaa agcctttcgg gcaatatgca ggactggagc atcgcggatg ggcaatggtt
      301 gattttttcg ggtatggcca ataatgccag cggtggcgca gtatttttgc agcaaagtgc
      361 tgaatttacg atttcaccac aaaatgaaac agggatgacc ctgtttgctg ataactcggt
      421 tagtggcgaa tataataatg gtggggctat ctttgctaaa gaaaactcaa cgataaatct
      481 tgcgaatgtt attttcgaca gtaatgttgc aggaggctat ggcggagcaa tctattctgc
      541 cggtaccaat gataccggtg ccgccgattt acgtgtcact aacgccgtat ttcacaataa
      601 catcgctaat gacggcaaag gcggtgcaat ttataccatc aataatgacg tctatttaag
      661 tgatgatgct tttaataata accaggcata tacatcaaca agttatagtg atggggatgg
      721 cggcgctatt gatgttacgg ataatagtac ggataacaca catctttcag gaaaaacgat
      781 aattaataat acctccttta ccaataacta tgcagaaggt tatggcggag caatttatac
      841 cagcagcacc acatccccgt atcttattga tatttctgtt gatgataatt atgaccaaaa
      901 caatggtgtg atgattgatg aaaataacag cgcctcggga tatgaccatt cggcgacggc
      961 agcggcgggg ggctttatgt atatcggtca tagtgtggct gaatttaaca ttgccgccga
     1021 taaaacgctg gtaataggca ataccagcaa cgatggcgcg attgattctc ttgccggaac
     1081 gggagtcatc gttaaagagg gagcgggaga gttagtcctc aatgccgaca ataatgcgtt
     1141 taccggcgag ataagtatcc agaatggcga ggtgacgttg gggcgcagcg atgagttaat
     1201 gaatgtcggc gacacgcact gtcagagcga tccgcaggat tgctttggtc tgatggtcgg
     1261 cagcactgtt cattctgagt atcaggcaga actgaatgtt ggcaatacac aacaaacgtt
     1321 tgtgcactca ttaaccggtt ttgctaatgg cattttgaat atcgacgcag gcggcaatgt
     1381 taccgtaaac cagggcggtt tttccggctc gattcagggt gaaggacagt tgacggtagc
     1441 gcaagatggc agctatctcc tgacaggggc gcagtcgatg gcattaaccg gcgatattgt
     1501 ggtggaagat aacgccgtat tgtcgctagc ggggaatcag gccgatttac gggcaatgca
     1561 gagcgatccg caatcgatcg tgctaaatgg cggagtattg gatctctctg atttcaccac
     1621 ctgggatggc gatagctcat acaatgacgg tctgcaaatc agcggcagcg gggggacagt
     1681 gattggcagt aatgatgtcg ttgatatcag cagtggcgat gatttacata ttggcggcag
     1741 tgatgccagc cagaatggcg tttatgtcgt cattaatgcg ggggatcagc gcgtaacgct
     1801 ggcgaataac aacggttatc tcggtaatac gcaaatcgcc tccggtacgc ttgaagtcag
     1861 cgataattcg caattggggg acacaagtta caaccgttca gtgatcttca cagatcccca
     1921 acaacacagt gaaatggatg tgacgactga tgttgacact cgctcggcta caacaggtca
     1981 gggcaggaat attgaaatgc gcgctgatgg cgaaatacac gttgaggatg gcgtagatac
     2041 gcaatggggc gggttgatgg cggatagtac cgggcaacaa ctggagagcg taagcacgtt
     2101 gactaaaagt ggcggcggta cgctggagtt gaccgccagc ggcactgcga cgtctgcggt
     2161 acgtgtggag gacggaacgc taaaaggtga agcggagaat attattcctt atgtttcttc
     2221 actgtgggtg ggggaagacg gtgtttttga aaccgggcaa aatcaggata ttcgctcaat
     2281 cgatgccacc tctggcggcg atatcgacat aaccgatggt actgtgttgc gattgacgca
     2341 gcaggatacg aaccaggtgc tggatgcctc gctgtttagc ggcgacggta cgttggtgaa
     2401 tgccaccgat ggcgtgacgc tgacaggcga gcttaatacc aaccttgaaa ctgacagcct
     2461 gacttatctt tccgacgtga cggttaatgg caatctgacc aatacgtccg gtgcgattag
     2521 cctgcaaaat ggcgtcgctg gcgacacgct gacggtaaat ggtgattata ccggcggcgg
     2581 tacgctactg ctcgacagcg aattaaacgg cgatgactcg gcaagtgacc aactggtatt
     2641 gaacggtaat actgctggca acacggccgt ggtaattaac cccattacgg gtattggtga
     2701 gccgacatct acaggtatta aagtggttga tttcgcagct gatcccacgc agtttcaaaa
     2761 caatgcgcag ttccgtctgg caggaagcgg ctacgtcaat atgggggcgt atgactatac
     2821 gctggtggaa gataacaacg actggtatct gcgatcgcaa gaagtcactc cgacgccgcc
     2881 gcccgatccg gacccgactc cagatcctga tcccacgccg gatcccgacc caacccccga
     2941 ccctggacct acgcctgctt accagccggt gttgaatgcc aaagttggtg gatattttaa
     3001 taacctacgt gcggcaaatc aggcatttgt gatggagcga cgcgatcagg ctggtggcga
     3061 tggtcagacg ctgaatttac gcgttatcgg agggcgttat cattacacgg cggcagggca
     3121 gttggctcag catgaagaca cttctacggt gcagcttagc ggcaacctgt ttagcgggca
     3181 ctggggtgat gatggcgagt ggatgcttgg gattgttggt ggctacagcg ataaccaggg
     3241 cgacagccgc tcgaatatga ccggaactcg cgccgataac cagaaccacg gttatgccgt
     3301 tgggctgacc tcaagctggt atcagcacgg taatcagaag cagggggtct ggctggatag
     3361 ctggctgcaa tatgcgtggt ttaacaatga tgtttccgaa caagacgatg gcgaggatca
     3421 ttaccactcg tcggggatta tcgcctcgct ggaagccggt tatcagtggt taccggggca
     3481 tggtgtggtg attgaaccgc agacgcaggt gatttatcag ggcgtgcagc aggatgattt
     3541 taccgccgct aaccatgcgc gcgtgtcaca atcgcagggt gatgatattc agacgcggct
     3601 gggtttacac agcgaatgga gtactgctgt tggtgtcacg ccaacgttag atctgaatta
     3661 ttatcacgat ccccatgcga cggaaattga agaggatggc agcactatca gtgacgatgg
     3721 ggtgaagcaa cggggtgaaa taaaagtggg agtcacgggc aatatcagtc agcgagtgtc
     3781 cctgcgcggc agcgtggcgt ggcagaaagg gagtgatgat tttgcccaga cggcagggtt
     3841 tttgtcgatg acggtgaaat ggtaatcgct gtctgaccgg cacttttgcc ggatgcggcg
     3901 taaacgcctt atctggccta cgggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3961 catcaggcat tggcacggtt gtcggatgaa tccaaacaac aacctcactt attctgcgtg
     4021 tgcagcatat agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg
     4081 taatgcagcc cggtcatatg ccgctctttt aaactggtct gatccaccca gcccagcaat
     4141 tctgccggtt taataaactt cttcacatca tgcgtgcctt tgggcaccat gcgcaaaata
     4201 tattcagcac caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa
     4261 aagacatcgc cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg
     4321 gggacgtgct ccagcatctc catgcaggtc actacatcat actgcccggc atgttttgcc
     4381 gcgtgctctt ccacggtttc ctgtacgtac tctacctgaa tgccgctttc cagcgcgtgc
     4441 aactttgcca cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg
     4501 cgcgccatac tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca
     4561 aataaaccgc cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcagc
     4621 ggtttgaact caccttcaag atcccaccag cgggaggcga cagcttcaaa tttagcgatc
     4681 tcttcgtggt ctacgttatg gtttaccggc gatttttcgg cattcattgg cacttctact
     4741 ccgtaattgg caagacaaac gagtatatca ggcattggag gtgaataaag cgtataggtt
     4801 tacctcaaac tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag
     4861 ggatagcggt tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga
     4921 gctgaagagc tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccaga
     4981 tgtccgagat ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg
     5041 caatgactgg aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa
     5101 ataccatccc catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt
     5161 ctcgctgcgt tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc
     5221 tgcggcggca atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc
     5281 cgatctcgaa aaagagacgg tcgatttcgt agataactat gacggcacgg aaaaaattcc
     5341 cgacgtcatg ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt
     5401 aggtatggca accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc
     5461 gtatatcgat gatgaagaca tcagcattga agggctgatg gaacacatcc ccgggccgga
     5521 cttcccgacg gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg
     5581 tcgcggcaag gtatatatcc gcgcccgtgc tgaagtggaa gttgacgcca aaaccggacg
     5641 tgaaaccatt atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa
     5701 gattgcggaa ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc
     5761 tgacaaagac ggtatgcgca tcgtgattga agtgaaacgc gatgcggtcg gtgaagttgt
     5821 gctcaacaac ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc
     5881 attgcaccat ggtcagccga agatcatgaa cctgaaagac atcatcgcgg cgtttgttcg
     5941 tcaccgccgc gaagtggtga ctcgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg
     6001 tgctcatatc cttgaagcat tagccgtagc gctggcgaac atcgacccga tcatcgaact
     6061 gatccgtcat gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca
     6121 gctgggcaac gttgccgcca tgctcgaacg tgcgggcgat gatgctgcgc gtccggaatg
     6181 gctggagcca gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca
     6241 ggcgattctg gatctgcgtt tgcagaaact gaccggcctt gagcatgaaa aactgctcga
     6301 cgaatacaaa gagctgctgg atcagatcgc agaactgctg cgtattcttg gcagtgccga
     6361 tcgtctgatg gaagtgatcc gcgaagagct ggaactggtt cgtgaacagt tcggtgacaa
     6421 acgtcgtact gaaatcaccg ctaacagcgc cgacatcaac ctggaagatc tgatcatcca
     6481 ggaagatgtg gtggtcacgc tttctcacca gggttacgtg aagtatcagc cgctttctga
     6541 atatgaagcg cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga
     6601 ctttatcgac cgactgctgg tggcgaacac ccacgaccat attctgtgct tctccagccg
     6661 tggccgcgtc tattcgatga aggtctacca gttaccagaa gccactcgtg gcgcgcgtgg
     6721 tcgtccgatc gtcaacctgc tgccgctgga gcaggacgaa cgtatcactg cgatcctgcc
     6781 ggtgaccgag tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa
     6841 gaaaaccgtc ctcaccgagt tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact
     6901 ggttgaaggc gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct
     6961 gttctccgcc gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg
     7021 caacaccacc ggtgttcgcg gtattcgttt aggcgaaggc gataaagtcg tctctctgat
     7081 cgtgcctcgt ggcgatggcg caatcctcac cgcaacgcag aacggttatg gtaaacgtac
     7141 cgcagtggcg gaatacccga ctaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt
     7201 taccgaacgt aacggtttag tggttggcgc ggtgcaggta gatgactgcg accagatcat
     7261 gatgatcacc gatgccggta cgctggtacg tactcgcgtt tcggaaatca gcatcgtagg
     7321 ccgtaacacc cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct
     7381 gcaacgtgtt gctgaaccgg ttgacgagga agatctggat accatcgatg gcagtgccgc
     7441 ggaaggggac gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata
     7501 attttacttc ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc
     7561 tgcaaagtct gggcttttgt cgtattaggg cgcggtaaag ttttgctgtg ctcgtaaaaa
     7621 atggctggct ttacacaagg aatgtggcaa tgagtggtga aaaaaaggcg aaaggctggc
     7681 ggttctatgg tcttgtaggt ttgggcgcaa tagtactgct ttccgctggc gtctgggcgt
     7741 tgcaatatgc tggcagtggg ccagaaaaaa cgttgtcgcc gctggtggtg cacaacaatc
     7801 tgcaaatcga tctcaatgag ccggacctct ttctcgacag cgactctctg agccagcttc
     7861 ccaaagacct ccttaccatt ccgtttctcc acgatgttct gagtgaagat ttcgttttct
     7921 attatcagaa tcatgccgat cgtctgggca ttgaaggcag cattcgccgc attgtctatg
     7981 aacacgatct cacgctgaaa gataagctct tttcgtcact cttagatcag cccgcgcagg
     8041 cggcgctatg gcacgataaa caaggccatc tttcacatta catggtgctg atccagcgca
     8101 gtggtttaag caaactgttg gagccattgt tgtttgccgc taccagcgac agccagttaa
     8161 gcaaaacgga aatcagtagt attaagctaa atagtgaaac catcccggtt tatcagttgc
     8221 gctataacgg caataacgcc ctgatgttcg cgacttatca ggacaagatg ctggtgtttt
     8281 ccagtacgga tatgctgttt aaagatgacc agcaggatac cgaagccacg gcgatcgcaa
     8341 gtgatttgtt gagcggtaaa aaacgctggc aagccagctt tggcctggaa gagcgtgctg
     8401 ctgaaaaaac gccagtacgc cagcgtattg ttgtcagcgc caggttgctg gggtttggct
     8461 accagcgatt aatgccttct tttgctggcg tacgcttcga aatgagcaac gacggctggc
     8521 atagctttgt ggcgttaaat gatgaatccg ccagcgtaga tgccagtttc gattttacgc
     8581 cggtatggaa cagtatgcct gccggggcca gcttctgtgt ggcggtgccg tattcgcatg
     8641 gtattgccga agagatgctt tcgcacatca gccaggaaaa cgacaagctg aacggggcgt
     8701 tagatggtgc agccgggctg tgctggtatg aagactcaaa attacaaacc ccgctatttg
     8761 ttggtcaatt tgatggctca gccgaacagg cacaactgcc tgggaaactg tttacgcaaa
     8821 atattggtgc gcacgaaagc aaagcgccag aaggtgtttt gccggtaaac cagactcagc
     8881 agggcgaagc gcaaatctgg cgtcgcgaag tgagttcccg atacggccag tatccgaaag
     8941 cgcaggcggt gcaacccgat caattaatgt cggattattt tttccgcgtg tcgctggcga
     9001 tgcaaaacaa gacgatgctt ttctccctcg atgacacgct ggttaataac gcgctgcaaa
     9061 cactgaataa aacccgcccg gcaatggtgg atgtgatacc cactgacggc atcgttccgc
     9121 tctatatcaa tccacaaggc gtggcgaaac tgctgcgtaa cgaaacgctg accagtttgc
     9181 cgaagaatct cgaaccggtt ttttataacg ccgcacaaac tttattaatg ccgaagctgg
     9241 acgctttatc tcaacaaccg cgttatgtca tgaagctggc ccagatggaa cccggtgccg
     9301 cctggcagtg gctacccata acctggcaac cgctatgaga cacgggctgc tggcgctgat
     9361 ttgctggctg tgttgtgttg ttacccatag cgaaatgctg aatgtcgaac aatccgggct
     9421 gtttcgcgcc tggtttgtac gcattgcaca ggaacagctc cgccagggtc caagtccacg
     9481 ctggtatcag caggattgtg cgggcctggt gcgatttgca gcgaacgaga cgctgaaagt
     9541 tcacgacagt aaatggctaa aaagtaacgg gttatctaac cagtatttgc cgccagagat
     9601 gacgctaaca cctgaacagc ggcaactggc gcaaaactgg aatcagggga acgggaaaac
     9661 cggcccctac gtgaccgcaa ttaatttgat tcagtacaac agccaattta ttggccagga
     9721 cataaaccag gcgctgcctg gcgatatgat ttttttcgat cagggcgatg cccagcactt
     9781 aatggtctgg atggggcgtt acgtcatcta ccacaccgga agcgccacga aaactgacaa
     9841 cggaatgcgc gcagtcagtc tgcaacaact tatgacatgg aaggacaccc gatggatacc
     9901 caacgattcc aatcccaatt tcattggcat ttatcgttta aattttctgg cgcgatagca
     9961 gcgtgcttgt tcctgtctct tgtgggaaca gggcttgcca atgctgatga ttcgcttcct
    10021 tccagcaact atgcgccgcc cgccggggga acattctttt tgctcgctga cagcagtttt
    10081 agcagcagtg aagaggcgaa agtgcgactg gaagcgccgg ggcgtgatta tcgacgctat
    10141 cagatggaag agtacggcgg cgtggacgtt cgcctgtatc gtattcctga cccgatggca
    10201 tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc aaccgcaata tttgggcgac
    10261 gggctgaaca atacgctgac ctggctgtgg gataactggt acggcaaatc tcgccgcgtg
    10321 atgcagcgta ctttttcttc tcagtctcgg cagaatgtga ctcaggcgtt acccgaatta
    10381 cagctcggta atgccattat taaaccttcc cgttatgtac agaacaacca gttttcaccg
    10441 ctgaaaaaat atcccctggt ggaacagttc cgttacccat tatggcaggc taaaccggtc
    10501 gagccgcagc aaggggtaaa actggaaggc gcatccagca atttcatctc gccgcagccg
    10561 ggcaacatct atattccgct aggcaagcaa gagccggggc tgtacctcgt cgaggcgatg
    10621 gtaggtgggt atcgggcgac gacggtggtg tttgtttccg ataccgtggc gcttagcaaa
    10681 gtgtcaggta acgagcttct ggtgtggacc gcgggtaaaa aacaggggga agcgaagccg
    10741 ggatcagaga tcttgtggac tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac
    10801 agcggtacct tgcagttaca acatatatcg ccggaacgtt catacattct gggtaaagat
    10861 gctgaaggcg gcgtttttgt ctccgagaat tttttctacg aaagcgaaat ctacaacacc
    10921 cgcttgtata tctttaccga ccgccctctg tatcgtgcag gcgatcgtgt tgatgtcaaa
    10981 gtgatgggcc gcgagttcca cgacccgttg cattcatccc ccatcgtcag cgccccggcg
    11041 aagctttcag tgctggacgc caacggcagt ctgttgcaaa ccgtcaatgt cacgcttgat
    11101 gcgcgtaatg gcgggcaggg gagtttccgc ctgccagaaa atgccgtcgc cggaggttat
    11161 gagttacgcc tggcttaccg caaacaggtc tatagcagca gttttcgcgt ggcaaactac
    11221 atcaagccac atttcgagat tggtttagct ctcgacaaaa aagagttcaa aacaggcgaa
    11281 gcggtgagcg gcaaactgca actgctttac ccggatggag agccggtaaa agatgcccgc
    11341 gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc
    11401 ggacgttttc ccgtgtcgct ggaaggcagc gaaacggtgt ccgatgatag cggtcatgtg
    11461 gctttaaatc tccccgccgc cgataaaccg agccgctatt tgttaaccgt ctccgccagt
    11521 gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc tcattgaacg cggcctggcg
    11581 cattactctt taagtaccgc cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt
    11641 tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa
    11701 gaccgcacga gccatagcgg agatctaccg tcaggcggca aatcctttac cgtcaatttc
    11761 gataaacctg gcaactacaa tctgacatta cgcgatgaag acggcttaat tctcgccggg
    11821 ttaagccatg cagtcagcgg taagggcagc acggcgcata cgggtacggt agatatcgtg
    11881 gcggataaaa cgctgtacca gccaggcgaa accgcgaaga tgctgattac cttcccggag
    11941 ccaattgatg aagcattatt gacgctggaa cgcgatcgcg ttgaacagca gtcgctgcta
    12001 tcgcatccgg caaactggct gacgttacaa cgtttaaacg atacccagta tgaagcccgc
    12061 ataccagtga gcaattcctt tgcgcctaac atcacttttt cggtgctgta tacccgtaac
    12121 ggtcagtaca gttttcagaa cgccgggatc aaagttgccg ttccccagct ggatatccgg
    12181 gtgaaaacgg aaaaaaccca ttaccagcct ggtgaactgg tcaatgtcga attaacctcg
    12241 tcgctgaaag gtaaacctgt ttctgcgcag ctaacggttg gtgtggtcga tgaaatgatc
    12301 tacgcgctgc aaccggaaat cgcgccgaat atcggcaaat ttttctatcc gctggggcgt
    12361 aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc gctctccagc
    12421 gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat gcttgaacgt
    12481 ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac cgataaacaa
    12541 ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg catcaccgcg
    12601 cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa
    12661 aatctctaca tgaagtggag tatgccaacg gtgtatcgca tgggcgacaa accggcagca
    12721 ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac taaatttgca
    12781 ggtgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat ttcgctgacg
    12841 cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg gcaagtacag
    12901 gacagcatta gcacaaaact gtcttttgtg gataacagct ggcccgttga acagcagaaa
    12961 aacgtcatgc tcggcggtgg cgataacgcg ctgatgttgc ccgagcaggc gagcaatatc
    13021 cagctacaaa gtagcgaaac gccgcaggag attttccgta acaatcttga tgcgttagtc
    13081 gatgaaccgt gggggggcgt gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc
    13141 tggcgttcac ttgccgatca tcaaagtgcc gccgctaacg acattcgtca gatgattcag
    13201 gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac ctggtggggt
    13261 gaagatggca atggcgacgc cttcctgacg gcatgggcat ggtacgccga ctggcaggcc
    13321 agccaggcgc tcggcgtaac gcagcaaccg gattactggc aacatatgct cgacagttat
    13381 gccgagcagg cagataacat gccgatattg catcgcgcgc tggtgctggc gtgggcacag
    13441 gagatgaatc taccgtgcaa aacgttgttg aaagggttgg atgaagctat cgcccggcgc
    13501 ggaactaaaa ctgaagattt ctctgaggaa gacaccagcg atatcaatga cagcctgatc
    13561 ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac catgacgttg
    13621 ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata tgcgtgggat
    13681 aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa tagcggtggt
    13741 gacgctaccc aggcggccgc tattttaagt ggtttgaccg ctgagcaatc cactattgag
    13801 cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga caatgcctcc agttgttttg
    13861 cctgcgcctg cgggcgcatg ggctaaacat aagttaactg gagggggcga agactggcgt
    13921 tgggttggtc agggtgtgcc ggacattctc tcttttggtg acgaattatc gccgcaaaat
    13981 gtgcaggtcc gctggcgtga accggcaaaa acggctcaac aaagtaacat tccggtgacc
    14041 gttgaacgcc agttgtatcg gcttatcccc ggtgaagaag agatgagctt tactctgcag
    14101 ccggtgacca gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc
    14161 agcgagcagg atgccgttct gcgctacggt caggtggaag taccgctccc gccaggggct
    14221 gacgttgaga ggactacgtg gggcatttcg gtcaataaac cgaacgctgg aaaacagcag
    14281 gggcagttgc tggaaaaagc gcgaaatgaa atgggcaaac tggcctatat ggtgccggtg
    14341 aaagaactga cgggaacggt cactttccgc catttgctgc gcttctcgca aaaagggcaa
    14401 ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac ctgcacagca aagtgttgcg
    14461 gcagggagcg aatggaccgg gatgcaggtg aaataagtga actggcgcag aatcgtctgg
    14521 ctattggcgt tggttactct gccaacgctt gcggaggaga cgccgttaca actggtgctg
    14581 cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat caggcgtgac caaagtctcg
    14641 gcattacctg acacgctcac cacgccgtta gggagtttgt ggaaactgta tgtttacgcc
    14701 tggctggagg atacccatca accggagcag ccctaccagt gtcgcggaaa ctccccggaa
    14761 gaggtttatt gttgtcaggc tggggagagt ataacgcgcg ataccgcgct ggtacgttcc
    14821 tgtgggctct attttgcgcc gcagcggttg catattggcg cagatgtgtg ggggcaatat
    14881 tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta ctacgctgaa gccagaaacc
    14941 tcggtaacgg ttaactcgct gcttgattca ttagcgacgt tacctgcgca aaacaaagct
    15001 caggaagtct tactggatgt ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc
    15061 ggcagtcggg tacgggtgaa aacctggagc tggtttgccg atgataaaca ggaaatccgt
    15121 cagggtggtt ttgccggctg gttaaccgat ggtacaccgc tgtgggtgac tggcagtggc
    15181 accagcaaaa cggtactgac ccgttatgcg acggtgctta atcgggtttt gccagtgccg
    15241 acgcaagttg ccagtgggca atgtgtcctg gtggagctgt ttgcccgcta tccgctgaag
    15301 aaaatcacgg cggaaaagag ttcgacggca gtaaaacccg gcgtacttaa tgggcgctat
    15361 cgtgtgacgt ttgccaatgg taaccacatt atgtttgtca gccacggtga gacgacctta
    15421 ctgagcgaaa agggcaaact gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc
    15481 agagttctgg atcgtgaggc gaaaagtacg ccacctgagg cagcgaaagc catgaccgtg
    15541 gcgatacgca cttttttaca gcaaaacgcc aatcgggaag gcgattgcct gaccatccct
    15601 gacagcagcg ccacgcagcg tgtttctgct tcgccagcta ccacgggggc gcgaacgatg
    15661 accgcctgga cgcaggatct tatctatgcg ggcgatccgg ttcattatca cggcagtcgc
    15721 gctaccgaag ggacgctttc ctggcggcaa gccacggccc aggcggggca gggagagcgt
    15781 tacgatcaga tactcgcgtt tgcttatccc gacaatagcc ttagccgctg gggagcgcca
    15841 cgctcaacct gccagttatt acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag
    15901 tggcggcgta tattacaagg tgagacgggg tacaacgaac cagacgtgtt tgcggtttgc
    15961 cgtttagtct caggtttccc ctataccgat cggcagcaga aacggctgtt tattcgtaac
    16021 ttctttacgt ttcaggatcg gctggattta acccatgagt atctgcacct ggcctttgat
    16081 ggttatccca ccgggcttga tgagaactat atcgaaacgc ttacccgaca attattaatg
    16141 gactaatgct atgcgaaaga tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc
    16201 tcacagtgaa ggtgtgcagg aagtcgagat tgatgcaccg ctctctggct ggcatccggc
    16261 agagggtgaa gatgccagtt tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat
    16321 ggcagatgat caaaatatct cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc
    16381 gggtaaagtt cagcagggcc ggctggtggt caacggtgcc agtatgccgc agagaattga
    16441 atccgatggt tcatttgcac gcccttatat tttcacggaa ggcagcaaca gcgtgcaggt
    16501 catcagcccg gatgggcaaa gccggcaaaa aatgcagttt tactcaacgc cgggtacagg
    16561 aacgattcgt gcacgtttac ggctggttct ctcgtgggat acggacaata ccgatctcga
    16621 tctccatgtt gtcacgcccg atggcgaaca cgcctggtac ggtaacacgg tgctgaaaaa
    16681 cagtggtgca ctggatatgg atgtcacgac ggggtacgga cccgagattt tcgccatgcc
    16741 agcgccgatt cacggacgtt atcaggtgta tatcaactac tatggcggac gcagcgaaac
    16801 ggaattgacg accgcccaac tgacgctgat caccgatgaa ggatcggtca atgagaaaca
    16861 ggaaacattt attgtaccga tgcgtaatgc tggcgagctg acgctggtga aaagttttga
    16921 ctggtgatta atggggttac cttcgccgtc gatgttcaag aaaacacccg ataactttcg
    16981 ctatcgggtg tttttattga ttaattcaac cgttcaatca ccatcgcaat tccctggccg
    17041 ccaccaatgc atagtgttgc cagccccagc gttttatcgc gtgcctgcat ggcatgtaat
    17101 agtgtgacca gaatacgagc accactggca ccgataggat gcccgagcgc gatggccccg
    17161 ccgttgacat tcactttctc agaatcaaag cccagggttt tcccaacggc aaggaactgt
    17221 gcagcaaatg cttcattagc ctcaatgaga tcaatatccg ccagttgcag ccccgccagt
    17281 tgtaacgctt ttttcgtggc aggtactggc cccataccca tcaatgcggg gggcacgcca
    17341 ccgctggcat aacttttaat gcgagccagg ggggtaaggc ctgctgccag cgccgcagat
    17401 tcttccataa tcaccagagc ggcagcaccg tcgttaatac cagacgcgtt cccggcggtg
    17461 actgttcctg ctttatcgaa ggccgggcgc aatgcgccta acgcttcagt cgtagaatcc
    17521 gctttcggga attcgtcttg actgaagacg aaggttttct tccgagtgac aacatttacc
    17581 gggacgattt cggctgtaaa agcaccggac tcaattgcgg ctgccgcttt acgctgtgaa
    17641 tgtagcgcca gttcatcctg catttcacgg gtaattccgt actctttagc cacgttttcg
    17701 gcggtaatcc ccatatgata accatgggtg gcgcacatca gaccatcgcg caggattacg
    17761 tcataaacct gtccgtctcc aagacgataa ccagagcgtg cttttgcatc gagtaagtag
    17821 ggcgctaaac tcatattttc catacccccc gccacaatgc tctgcgcctg acctgcctga
    17881 attgcctggg cggcaagcgc cacactttta agacccgaac cacacacttt attgaccgtg
    17941 aatccgcaca ccgtttcagc cagtccgctt tttagcagcg cctgacgtgc cggattttgc
    18001 cccagtccgg cttgcaacac gttacccata atcacttcat caacgtgtag tgaatcgatt
    18061 tttgcacgtt caatggcggc tttaattact gtcgccccca ggtcgatggc gctggtggaa
    18121 gcgagtgaac cgttaaaact accgatagca gtacgtaccg cactgacgat gacacaattt
    18181 ttcattttat attccttctg tctgtagggt tgccgtcaga acagcgttaa accaatgacg
    18241 aaaatgacgc cggagaagag caaggcagtg atgcaatagc ccatgatgtc gcggacaccg
    18301 agtccggcga ttgccagtgc tggcagcgcc cagaacggtt gtgccatgtt catccattgc
    18361 tcgccgtagg cgatcgccat taccgattta ccgagatcag cgcccagcgc ctgggctgcg
    18421 ggcatcacga acggcccctg aataacccag tgcccgccgc cagacggaac ggcgaagtta
    18481 atcagtgcag aactaaaaaa ggtcatgatc gggaaggtgt ctttgttcgc aacattgatg
    18541 aagaattcgg taatgagtcc gcccagaccg gaatgctcca tcatcagttg gatcccagcg
    18601 tagaagggga attgcaccag aataccggca gtactgcgtg ctgccgcgct gatagcacgc
    18661 atataagcca ttggcgtttt atgtagcagc agacccgcaa tcataaacat caggttgacg
    18721 gtattgatgg tgatgttgaa gccatgttcg ctgaagtaca tcgcaaggta agcgataccg
    18781 agtgcgccga tgatcaacgt cagaatgcgg ctttcttcca gtcgctcgga tggtggggca
    18841 tctttcggta gctgcttttg aaaatcagcc tcttccatga gtagtttcgg atcgatactc
    18901 accacgtcag acggttttgg catcatcatg cgggtgataa atggcatcac cacaatcaac
    18961 gccacagtga tgaaaatatt aaaaccactg aacagagtat cgcccaccgg gatcagcccg
    19021 gcgatatgct caaccgggtt gcccggtgtt gcagccaaca gaggcattga gccagagaaa
    19081 ccgccacccc aggtgagaaa accaatgtag gcgcaggcaa tgagcaacgg ataatcagaa
    19141 ccgggtactc gccgggcgac ttcacgggcg aacattgcac cgacaaccaa accaaatccc
    19201 cagttgatga cacaagcgac tgaaccgaag aaagtaacca gcatgacgcc ctgtacgggc
    19261 gtctttgcgg cggaggcggc agtacgcagc aaacttttca ccggagcgga gctggcaagg
    19321 gcatgaccgg taacgatgat aagcgccatc tgcataccaa acgccagcaa gttccagaaa
    19381 ccatcacccc acattttcac catgctgatc ggcgtttgtg gtgttaacca aagcgcgatc
    19441 acgaatgtta gcaatgtcag caacatggca aagatcagtg gatcgggaag ccaccggctg
    19501 acaaaacgcg tcataaaacg cgatatgcga ccaatcataa atcaccccgt tgcgtattca
    19561 gatcggcggc aacttcaaac cgagcttctg ttttggcacg cacggtggct aaatcacacc
    19621 cgtcggcaat ttcggtgagc cacattttgc cgtcaataaa acgaaagaca gccagttcag
    19681 taaccagcat atgcaccgca tgttgcgcag tgagtggcat ggtgcagcgg cgcaaaattt
    19741 ttgctgaacc atctttggcg caatgttcca tggcgatgat cactttgcgt gacccggtca
    19801 ccagatccat cgcgccaccc ataccgggca ccattttccc aggcactacc cagttcgcga
    19861 ggtttgcttc ttcgtctact tgcaaaccgc cgagcacgca ggcatcaata tgaccgccgc
    19921 ggattagcgc aaatgacatg gcgctatcaa acatggctgc gccgggtaaa atgccgcacg
    19981 gttgtccgcc agcgttcacc agatctggat gcgctgtcgt gaccgggcct aaaccgagga
    20041 agccgttttc cgattgcaga gtgatatgaa taccctccgg taaataattg gcgaccattg
    20101 tgggtaaacc gatccctaag ttaacgatgt caccatcacg aagctcttgc gccacacggc
    20161 gcgcaatacg ttgtttcgca tccattattt gctctcctgt gaaacgatga tgtggtcgat
    20221 aacggcacca ggggtgacaa tatggtcagg ttgcagctcg ccggtttcga ccagttcatc
    20281 tggctctacc agcgtgatat cagccgcaag ggctatcagg gggttaaagt tgcgggcgct
    20341 aagttgatag gtaaggttgc caagtgtgtc gcaacgatga gcgcgaatta gcgccaggtc
    20401 ggcgcgcagc gggcgttcga gcagccaggt tttaccgtcg agtgtcagtg tctgtttgcc
    20461 ttcctctacg acggtgccga caccagttgg ggtgagaaaa ccaccaagtc cagctccgcc
    20521 acagcgaatt tgctcgatta gcgtaccttg cggcaccaga acgacgtcca tctcaccaga
    20581 tatcatgcgc cgacctgttt ccgggttggt gccgatatgt gaagcaatca ctttgcggac
    20641 tcgaccattg acgatgagcg gaccgatgcc ggtatcaaca aacgcggtat cattggctat
    20701 caatgtcagg tcgcgaacac cagattccag taatgcttca accaggcggg atggagtgcc
    20761 aatccccata aatccgccca ccatgatggt catgccgtca cgaaagaagc cggtggcgtc
    20821 ttgtaatgtc atcaattttg ttttcatttt ttatccctct tgcatacacc gttgtgggta
    20881 cagtcaggga atagcaaggg gagtgccaga atgagattat tgaatgttgt tatttaacta
    20941 attgaataga aaggaataaa ttagatgggg attcataagg agaatgagcg gctatgcaga
    21001 aaattgcgca ctgtgcaaat ttctgcatag caagttttgg cgttatacat ccgccggatc
    21061 gataccgtat tcctggagtt tatacatcaa tgcacggcga ctgatgccca gcattaacgc
    21121 agtgcgggtt cggtttcctt cttgttgttc cagcacttcc ataatgatgc gtttttcgac
    21181 gcgtttaatt tcctctttta aattacgctc accgacaggg gctgtttttg cctcaccagc
    21241 attacagact ggctgacgaa tctgcggcgg aagatcctca gagaaaatga tcgggcctga
    21301 gttcatcacg acggcgcgtt caatgacgtt ggaaagctct cgaatattac ccggccatga
    21361 ccaggcggta agcagcgaca ttgccatcgg atcgatgtcg ataatatcgc gctgattctc
    21421 actactgaat ttttgcaaaa agtgattagc taacagggaa atatcttccc ggcgatcgcg
    21481 cagaggcggc agtattaaat gaataacgtt aaggcgataa aagagatctt cacggaaggt
    21541 gccttctttt accattgcct gcaagtcacg gttggtggca gcaatgatgc ggatatcaac
    21601 ttttatggtc tgatgaccgc caatccgttc aaattcccgt tcctgcagaa tgcgtagtaa
    21661 tttggcttgc agtacgagcg gcatttcgcc aatttcgtcg aggagcagag taccttcgtt
    21721 ggcacgttca aataatccct gacgcaaggt ttgtgcacca gtaaatgcac ctttttcatg
    21781 accaaacagt tcactttcga gcaacgattc cggcagcgcc gcgcagttga ctttaatgaa
    21841 cggccccttt gcccgccgcg aattgtagtg aatcgctctg gcaatcaact ctttcccggt
    21901 gccgctttca ccgctaatca agacgctggc ctgagaaagg gcaattttgg cggtgtcttt
    21961 gcagatatcc atcatcgccg ggctgttggt gagaatgtgc ccccattgcc agctggtgct
    22021 cagtgcctgg tgcagatgac ggatctcttt tttcattgac tggagttgta aagcgcgctg
    22081 aacgattaaa ttcaactcat cgagatcaaa cggtttaata acatagtcga aggccccgca
    22141 gcgtagcgct tcgacggcgg tttccacttc agcataggcc gtcatcagaa taacgggtgt
    22201 ccgggtctca ttactgcgca tctcctttag tgccttgatg ccgtccatct ctggcatgcg
    22261 gatatccatc aacaccacgt cagggtgaat atcagcaaac aggtgtaatg ctgtgcgtcc
    22321 gttgttcgca caatgtgttt cgaaaccttg tagtgcaaaa gcggtgctca gcatacggcg
    22381 aacattatct tcatcatcca caataaggat gcgattaata gcagtcatac agtctgattt
    22441 ccctgcgggt tgatcggtaa aataagcgtg aaggttgcgc cgtagcccgg caaactggcg
    22501 acgcgaatat caccctgatg ggcattaatg atgcgttgac ttaacgccag accaagcccg
    22561 gttcctgagg ctttggtggt gaaaaaggga tcgaagatct ttttttgcag cgagagatca
    22621 atgccacagc cgttgtcctc tatcgaaatg gcctgttgtg agtcgctgta ttgccaggtt
    22681 cgaatgcgaa ttttccctcg tgcactgata gcctggacgg cattgatcag gatattcagt
    22741 agtacctgtt tgagcagttc acgatcggcg ttaatcgggc ttaattcatt atccagttcg
    22801 cttatgaagt cgacccgcgc ttgtacgccg gcggtttgta ccagtaccag agtttcttca
    22861 accaatgcat tgaggctgac ttgttgccat tgactgtggc gtgggcgtga aaattcgagc
    22921 aattgctgaa taactttgtt aattgaatcg atttctttga gtactacgga cagatattcc
    22981 tgatgtattg ggtcacgggt ttgttggcgc aagatctgta cataaccacg aatagctgtt
    23041 agcggattac gtacttcatg cgccacgcca gccatcagct cacccagtgt ggcgaggcgt
    23101 tctgcttgcg ccatgcggcg ctgggtttct ttgcgggcag ttaaatcaga gaaaatcacc
    23161 aaagcaccta tcatttcacc gtgcgtgtta tgaatacgac tggtcgtgac actgagttca
    23221 atggtgcggt cacggcctgg aaaactgatc tccagtgcta catgttcggt gccatgttcc
    23281 agcgtatcca gtacgggact gtagaactga gtattgtcga acaacatgga gtaaggctgc
    23341 cctaccagtt catgacgttg atagccagtg atgacttctg ctgctgggtt catggtggtt
    23401 acatcaccct ggcggtcaat ggcaatgacg ccatcggcag cgttttcaat aatcagatcg
    23461 ttaagtgttc gcgtttcacg cagtgcctgg gcgaggttgt taacactctg actgatttgc
    23521 cccatttcac cgggcaattg tggtaatcga gtgggaatat tttgagccag agtcgagagg
    23581 ccatcggtga tgatatctat attggcgctc aggcgacggg agaaaaggac aatcaacagc
    23641 aggcttatca gcaaaccggc ggtgagcaca atgataatcc tcacatccat tttccaggcc
    23701 tggcggcgaa tatcttcggt taattcattg gcccagatat agccgaggat ttcaccatta
    23761 cgctcaatgg gaatcattga attcaaaata tcgccgcgca cctgtctgcc tgaataaacc
    23821 aaaggggtat ttgtacgcat gacttcgcga ccagggtgat ctgcggcaat ggtaacgccc
    23881 acattattct gatatagcgc tgaaggcgcg taggtgatta tcgcatccag cgttttgttg
    23941 taataaccag cgccgatgcc agggaaggcg tgagtgatat tttcggtaat gggggcaagt
    24001 tctgcattta atgcgcggat acgctcctca cgcggtaagt cgatgtagag atcgtagcga
    24061 ttgcctagcg cctgattaag caggttgacc acggcagaaa gttttttctc tttttcagat
    24121 aagactgctg aacgtccttc cgtttctacg atataaccaa tagtaagcgt tgggacaatg
    24181 accatcagga ttgccatcag gatcatttga ttacgtaagc ggcgtggata aatccacttc
    24241 atataatgca tgctgaaatc cactagtctt gtccggtata tgacgattat cagaggttaa
    24301 ggtgatgatt tctcggcggt gtatcatatt ccagagaaga gagaacattg cggtaacacg
    24361 cttttaccgc taccttaacc acactccatc ggtcacctga ggcggagctt cgcccctttg
    24421 aaataccttg cttcttttcg tacaaccctg aaagcctcgc gctacatgtt cagagcattg
    24481 gcgttagtgc tctggctgtt gattgctttt tcatccgttt tttacatcgt taatgcgtta
    24541 catcagcgag aatcggaaat tcgtcaggaa tttaatctga gttccgatca ggctcagcgc
    24601 tttattcaac gcacctctga tgtgatgaaa gagctgaagt acatagctga aaatcgctta
    24661 tcggcagaaa acggtgtgct ttccccgcgt ggacgagaaa cgcagacgga tgtgcctgcg
    24721 tttgaaccgc tatttgctga ctctgattgt tccgcaatga gtaacacctg gcgaggttct
    24781 ctggagtcat tggcgtggtt tatgcgctac tggcgcgata atttttctgc ggcttacgat
    24841 ctcaaccggg tatttttaat cggcagcgat aacctctgca tggccaattt cggtctgcgt
    24901 gatatgccag tggaacgcga taccgcgtta aaagctttgc atgaacgcat taataaatat
    24961 cgaaatgcac cacaagatga tagtggcagt aacctctact ggatcagcga aggtccgcgc
    25021 cctggcgtcg ggtattttta cgccttgacg ccagtttatc tggcgaaccg gttgcaggcg
    25081 cttttgggtg tcgagcagac catccggatg gagaactttt tcttacctgg tacgttgccg
    25141 atgggggtta ccattcttga tgaaaatggt cataccctga tttcgcttac cggaccagaa
    25201 agcaaaatta agggcgatcc tcgctggatg caggaacgct cctggtttgg ctatacggaa
    25261 gggttccggg agctggtgct gaagaaaaat ctgccaccct catcgctaag catcgtgtat
    25321 tcggtgccgg ttgataaggt gctggaacgc attcgcatgt tgatccttaa cgcaattttg
    25381 ctgaatgtgc ttgccggagc tgcattgttt actctcgcgc ggatgtacga gcggcgtatt
    25441 ttcattccgg cggaaagcga cgccctgcga ctggaagaac atgagcagtt taatcgcaag
    25501 attgtcgcct ccgcgccagt gggtatctgc attttgcgta ccgctgatgg cgtcaatatt
    25561 ttaagtaacg aactggcgca tacctatctc aatatgctta cgcatgagga ccgccaacgg
    25621 ctgacacaaa ttatctgtgg gcagcaggtc aattttgttg atgtcctgac cagcaacaat
    25681 accaacctgc aaatcagctt cgtccattcg cgctatcgta atgaaaacgt ggccatttgt
    25741 gtgctggtgg atgtttcttc gcgcgtgaag atggaagagt cgttgcagga gatggcacaa
    25801 gcagcggaac aggcgagcca gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg
    25861 cgaacgccgc tgtatggcat tatcggtaac ctggatctgt tgcaaaccaa agagttaccg
    25921 aaaggcgtcg atcgtctggt gacggcaatg aacaactctt ccagcctgtt gttgaaaatt
    25981 atcagcgata ttctcgactt ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt
    26041 gagttttcac cgcgtgaagt gatgaaccac atcaccgcca actatttacc gctggtggta
    26101 cgcaagcagc taggcttgta ctgctttatt gaaccggatg tgccagtggc cttaaatggc
    26161 gacccgatgc gtttacagca ggtcatctcc aacctgttga gtaacgccat aaaattcacc
    26221 gataccggct gtatagttct gcatgttcgc gctgatggcg attatctctc tatccgtgtt
    26281 cgcgataccg gcgtagggat tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc
    26341 caggtcggaa cgggcgtaca gcgtaacttc caggggaccg gtctgggtct ggcgatttgt
    26401 gaaaaactga tcagcatgat ggacggcgac atctcggtag attcagaacc gggaatgggc
    26461 agccagttta ccgtgcgtat tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg
    26521 gaagggttga gtggtaaacg ttgctggctg gcggtccgca atgcgtcgct ctgtcagttc
    26581 ctggaaacca gtttgcagcg cagcggcatc gtcgttacaa catacgaagg gcaggaaccg
    26641 actcccgaag atgtgttaat cactgacgag gtagtgagca aaaaatggca gggtagagcg
    26701 gttgtgacct tctgtcgtcg ccatattggt attccgctgg agaaagcgcc aggagagtgg
    26761 gtacacagtg tggctgcccc gcatgagcta ccggcattgt tggcgcgtat ttatttgatc
    26821 gagatggaga gcgacgatcc tgctaacgct ctgccgttaa cggacaaagc ggtcagcgat
    26881 aatgacgata tgatgattct ggtcgtggat gatcatccga ttaaccggcg tctgctggca
    26941 gatcagttgg gatcgttggg ctatcaatgt aaaaccgcga atgatggcgt cgatgcgctt
    27001 aatgtactta gcaagaatca tattgatatc gtgcttagcg acgtcaacat gccaaatatg
    27061 gatggttacc gcttgacgca acgcattcgt cagttgggac tgacgttgcc ggtaatcgga
    27121 gtaactgcta atgcgttggc tgaagagaag cagcggtgtc tggagtccgg tatggacagc
    27181 tgcctgtcga agccggtaac gctggatgtg ataaaacaga cgctgacggt atatgccgag
    27241 agggtcagga aatcgcggga atcgtaggac ggataaggcg tttacgccgc atccggcaat
    27301 ccgtgcaaat gccagataag acactgacgc gtcttatctg gcctacaggt gattagtctt
    27361 tatctgccgg acttaacgtc acagaagaga gataattcag cagagcaata tcgttctcga
    27421 cacccagctt catcatcgca gatttcttct ggctactgat ggttttgata ctgcggttca
    27481 gctttttagc gatctcggtc accaggaaac cttccgcaaa caggcgtaga acttcactct
    27541 cttttggcga gagacgcttg tcaccgtaac caccagcact gattttttcc aacaggcgag
    27601 aaacgctttc cggggtaaat ttcttccctt tctgcagcgc ggcgagagct ttcggcagat
    27661 cggtcggtgc accttgtttc agcacgatcc cttcgatatc cagatccaat accgcactaa
    27721 gaatcgccgg gttgttgttc atagtcagaa caatgatcga caggcttggg aaatggcgct
    27781 tgatgtactt gattaaggta atgccatcgc cgtacttatc gccaggcatg gagagatcgg
    27841 taatcaacac atgcgcatcc agtttcggca ggttgttgat cagtgctgta gagtcttcaa
    27901 attcgccgac aacattcacc cactcaattt gctcaagtga tttgcgaata ccgaacaaga
    27961 ctatcggatg gtcatcggca ataattacgt tcatattgtt catgtacttg cacctatgta
    28021 ttaactattt gattttaaaa atatattcta tgctttgttt agtcgttggg gcacctgtgg
    28081 gacataagta catagctttc tgtttagtac ttctacctgg gactggttat tgtctttcat
    28141 ccatgcaccg taaacattgt aaaccatctg ggcattcgca tgtcccatct ggttggctat
    28201 gaagtttgga tttgctccgg cagttaaagc ccagcaagca taagtatgcc gagactgata
    28261 cgcttttcta tggcgaattt ttgctcgttt taatgcagat tcccatgttc tgttgatgga
    28321 tgttacggca taatgaactc ctgagttacc tgtgcgatct aatatctgag gattgaaaac
    28381 aaagctgcat gagtgagtgc ttgtccggcc atactctcgc agctttactt ctatctgata
    28441 ttgtttacca agcctggtta atgtcgcctg attttttaat acgtcaattg ctggctgtat
    28501 gagttgtatt accctgtttg ttcctgcatc tgttttgggt agtgtaaatt cctttgtctg
    28561 agtgtaattc cttttgacgg tcaaggtacc cgcttccaga tcgatatcct cccatgccag
    28621 cccacacagt tcgccatgcc gcatcccggt atatacagcc agcgaccaga aatttttagt
    28681 ttgctggtgg tggcacgcat caatcagtct ggcaaactca tcttttgtta gtggatcggg
    28741 aggtgttttc gctcttttca gtgatggaag ctcactgaaa gggtttttgc ctatataccc
    28801 attgtttgcc gcaaaacgga acatcccgga gataattgtc atatagctat ttacagtagg
    28861 tacactacga cctttaatag gcttcctctg attttttcct ggcaactgat atcccgttaa
    28921 taattcttta cgaaaaatga gaacatcttc ctgtgtaatt gatgatgcca gtttgttccc
    28981 tccaaggcag tcaaggcaat ttcggatagc tgatttgtat ctgaccatgg catttgctgt
    29041 tatttccatt ttcttaagct caagccattt ttccgaaagt gccttaatgg ttatctcttt
    29101 ttttcccaga ccaaaatgtt tcaggttagg ggaattaggg aactgcgcgg cgtagtcgaa
    29161 gctccccatt cttattgcaa aacaaacgga agtgcgaagt tcacctgcga tcttccggtt
    29221 tttggcggtg tcaggaacac cgaggttttc tctgacacgt ttgccgttat agtgaaacca
    29281 tatgcggagt gatccgccat ggttttcaac gcctgtcggg tatgatgcgt tactcattaa
    29341 acctcccaga cgtccaggag cattaacagg ttaaccggaa cttgcatttt tggcacctgg
    29401 ttgtttctgg ttttcgatcc agcgcataat ttcctcaatg ttgtatacac attcactgaa
    29461 aggcccgggc tctccttctg ctgcgtaatg acggtattct ttccccagta accaggattt
    29521 ttttcttgct ctggcaatgg ttcctgattt cagtccagtt gcagcaataa gggttctttc
    29581 tgtgcaccat ttgctgggcg ttatctgata gatgattgtt tccattcctg actcacacta
    29641 tcttcaggcc acggcagtgg caccacacgt caaacattcg ctttacaact tcacggcagt
    29701 agaagccgtg agcatctctg gttaaatcgt atcggtaccc gtatctgagt cgtatccata
    29761 tttcaaactc gcgattcacg acttattctc ctgctgtctt tatgccagtt cattcggttg
    29821 cgctggggcc ctggccttcc gtggcctgta tttgcggcgt tttctggctt caatgcgtat
    29881 ctttgtcacc ctgactggct ttggttcgtg ccggaacact ttgtttctga tattacgccg
    29941 tgtattcatc atccagataa aacgctgggt atggtcgcat ccgtcatcaa cggtaattac
    30001 tgtgcggacc agttcatcgt taatatcaac catttcaccc attcactgag ccttcatacg
    30061 cgaatttcgt ggcttccatc agttgcgtcc atacccattc aagaaattgt gcgttttctc
    30121 ctgagcggtc atattcatcg acgctgcggg gcttgatggt cgccagtagt ggcacaatcc
    30181 ggtacttcat ctgttcggtt aacttgtccg gtacaagtcg ccagccgtta tatttcgatg
    30241 gaacatggtg gtattcagcc aggaagcgtc gtatatcgtt tttcatttcc tcgcatgagt
    30301 tctgatcatg aagtgcagcg gaaagccagc gtccgatata ttcagggatt cgcacatcaa
    30361 gtactttttc tgccgtcgtc agcattgcct gtcggcaggc gttccagccg ttgacatagt
    30421 gacgtgttgc atcccatgaa acctgcacat cagggtaagc atcctgttct gttctcactg
    30481 gcggtatctg aggaacagtg aatagtggac tgacgatata tttatccaga gagcctcccc
    30541 gctcttctgc aagactttcc cacgatgtca ttgtggggac cctgtaacct gcttttttgt
    30601 gcgtatacat ccatgccact ctgccgttat catctgcggc ggcaatgcgc tgccaggcag
    30661 gataaagcgg ggtgtatgtt tctctgtcat tacgggcgtc aggcatggca tcaatactgc
    30721 aaacttcccc cgtaaggtta ttcagccagg ccttcgggct aatctctgac gttacgattt
    30781 catccagttt tttctccaga ttttctatgt gactgattag cgcaaggata actggcggag
    30841 tggcaaggga gtgaaatgct tccagatcac atcccttgtc gccatatacg gtattttgtg
    30901 cggcgtcacg cagtgctttc agatcagctt ctttcatcgt tcagactccc ttgttgctgt
    30961 tttgtaggcg cgcaggatgt cgcgtgtttt tcctgataat gacgacttaa ccaggaaaaa
    31021 gccgctccgg ctttctgtga tgtccggcgt gcacagaagt accgcatcaa cgacacgatt
    31081 atgttttcgc aatgtcaggc gggaactggt gataatcagt tttgcctgtt cgccgtaatc
    31141 ggtgaatgaa ataatcatct gatggcgatc tccgttatga aagtgttcct gctggttcaa
    31201 tgctgataat ttctgctgca ataagcatac ctgccagcca tgcttcgccg agcatgtcct
    31261 catccgtaca tgcaagatca ccgatctgta ttgtggccat aatgttagtt tcaccggtat
    31321 attcatcctg tgcttcttca tacggcaggt cttcataaag gttttcaatt gcgcaggtta
    31381 ttacatccag tcccgtcaga ttgccgacaa cgtccacttc aaatgtttcg cggtactccc
    31441 attgcccgag tgtcagacga accgtctgtc ttgccatgcg cccgcataat gtcatgttcg
    31501 ggtcgtagtt catgattgcc gcagtagtgc tattcatgtt ctgcctccgg tgtgtaaata
    31561 gctttgtcgt gtttaaattc accgttccag tttttcttca tagggagttc accgttcatg
    31621 taaaactggt ataaacggtg gcatcctttt tccagcaata cgggggtgaa tttcgtgaat
    31681 gttgctttcc cgtgtggagt gatctgtact gcttcttcgg tcaggtatct gtcacgggca
    31741 taagaggccg tgcgccagcg gggtgttttc tccggatcgc gttgttcgtt gaatacccat
    31801 tttcggccag acagccacca cattatctgg ctggtattta cgccgttcag cgcacggcag
    31861 aactggggaa cagttatgcc tttagagaaa tgtttttcca gactgtctac ggttgcagag
    31921 agcgttttat tttccagggc catagcttca gcccgttctt cggcctcaat aaccatcaac
    31981 gcgagttcct tgcgagtgag tacgcgctga acatcgatgt tttcccgctg agtgaagtag
    32041 aactccacaa ggtcttcgtg atagttccac gcctgatccg tttccagcaa ttttgcgtgg
    32101 ttggctgcgc cgcgttcggt ccagaggatg agggatcgaa cattgctggc aattttcaca
    32161 gagtagcttt gagatactct gtgcttaagt tcacgtagtt catcgccttc cagcaaaaaa
    32221 taatgctttc caacaacgaa tcgttcttcg tttcgggaat gattctggcg aatacggatt
    32281 ggtgttgctc cataacctgc cgccagttgt tcagtggtca caacgcgttg accgcgatat
    32341 tcgataattt gcaaatcacg ggctgccacc ggcacaagtt cagttgattt tttcatcatt
    32401 acacctcacg aaaagctgat tagtggaaca ctctggtttc cggttctgag ttgtcagtgg
    32461 cttcactgat aagcccatcc agaatcatgt gcatcgctct gtaaccttcc attgtgatgc
    32521 cggttttttc gccgtcgccc agagcgccgg tgaatgcttt gtacataaga atgcttttga
    32581 gcagccctga ttccgctccg taagtttcta tgagtgccca ttccatatag ttctgcatag
    32641 cgagacgtaa tggtttcagg tagattgtca tgccaccgtt gtggcctctg tacatggtgc
    32701 ccttgtctgt accgccattc tcgttaagca cttcggtcca gccgtttctg gtgagctgtt
    32761 cagagaagaa catggcggta taaagccagc gccagtacat gattgtcttt tccggcgcga
    32821 gctcacctgt catgcctttt tcttctgctt cactgatgca acacactatt tccagaccat
    32881 gccagatcgg ctcgtcaaaa cagccttcat cgagcatttc cagtgctttg ttgatatcaa
    32941 gcagtccgta aggtgtgtgg ataccttcag cggtaagaaa aaataactgt tcagttgcag
    33001 tcatggtttg ctctcttctt aaatggtctt tgcgcgacaa gccggcaata ctgatcaatc
    33061 caggcgttgt atttttccat ccaggcatca aactgttttc tgcgtgcaag gatgcgcaac
    33121 aggcgatgaa aacaacgccc gtgagctctg tagtattcgg gcgtaatttc tcccggatga
    33181 aagacttctc catattccgg atgcatcagg cactggtcag gcagacgttt ttcaaaacct
    33241 gaactttcga ataccttcgt tgtcataaaa tgagcgagat gtatgatggc cgtttttcgc
    33301 gtgaagcatt tcttaacttg tccgtggcgg cagactctgt aaacagggcc gcaatcgagt
    33361 tcgtatttat gaaaagcacg gtcgattccg gcattattta ttgcactgta ttccgcatca
    33421 ctgaagaaag gtggaaatac gatgtatcct tccattgtca gcgccagaac gcgcggatcg
    33481 gtttctgtca gccattcgtt ttctgtgaat tcttcagaat taatttcaat gatttcaggt
    33541 tcttctttca tggttactcc gtgttcatcc ggtgttaatt acttttattc actctgagcg
    33601 cagccagaag atgtggctca gcaattcgtt tgttgcgata tatctgtgat acagcctggc
    33661 cttttcaggt tgtaaaaatc cccgacaata aagctgtaaa ttaattactg ttcttaaata
    33721 atgttattcg ttttcagtcc ggcacagatg cttttctgct ttgtctgctg tacaggataa
    33781 tgagcggaaa agacaattca tcatgtttag cgcatctggt gaaccaatat caccgctgtc
    33841 aataatcacc tgtaacaaag acaggctttc tgttatctcg cagtggacat tatcgagtac
    33901 ttcaacgcaa ttattcattt ttcattatct ccgttattcc gggggaggag ctatgctgtt
    33961 aatgattaac gcatctggta atatgcccct ctttaatgta atcgatgcat tcattcaaaa
    34021 gttcatcaat gattaacttt cctgattcag tcagatactc accatgttgg ttgataccga
    34081 cagcatcctg gtatgcagtg cgtattgctg tttcaccatc tttctgccca aactcaccac
    34141 gggtaacgcc ttcaaatcgt aatagcaact ggcttatgaa ctgttctgtt atttcaatag
    34201 ttgttaactc tccatcatga cgctttacag tgaggagatt acttcctgtt tttcgtttca
    34261 tccgccgcag tgctgcaacc gttatgcggc gacgatatgc agaaatgtat tcatctttca
    34321 tttgttattt cccgtatgcc tttttgagaa acaatactgc aactgaccag tatccggcat
    34381 cagccattaa taaagcggtt ttataggcat gtttgtcttt catgtgtcat ccccatttta
    34441 aaatagtgag aactcccgac cgcagaccgt catgttttta tgtatgattg tctgtttaat
    34501 ttcctttatt cgttctcaga gcatcaacat actcgtaagc cttttcacag gttttattca
    34561 tggaacgaat catacaaagt aagaaatcat ctgtctctcc gttatcacca gagtttttaa
    34621 atataaactc aagcattgac gtgttttctt ttatttctgc tgccacttcc tcaagcataa
    34681 tcaggggagt tttcatgttc tttgttcctt aaatgcatcg catgcgcttc tggcgtattg
    34741 ttgtgccagt aaaaatatgt catccgaaag ttcatcacat tcttcatcac cggaagccga
    34801 aatgattaac cccgcttcaa gcagtactgc aatgtgatga aaagctgttt ccggttcgtt
    34861 ggtgaggcct ttgaacattt tcatcttacg attcctttgt cgtgtcagaa tggaaacttg
    34921 taagtaacca catcggatcg cagtcaagta tatttgccag aggaatcacc tggcttgcca
    34981 gaggttcagc cttgccgttc tcccactgga tgatggtttc ttcatcaact ccaagcagcg
    35041 tggcgagttc agcagtggat agaccgcagc tttcgcgttg ggtgcggatg ccatcacggc
    35101 aatctttttt gtttctcgga ggtaacagaa tatgttttag ttgttggatt ttttcgattc
    35161 caacctcaag gagtacgtga cggtttcttt ccgatacaga ctccagactt tcatgaagca
    35221 gcgattctac ttgttcgata agcatcaatt gtgaagtaga cataaaaaaa cctcgtaacc
    35281 aatatttcga aatctaggtt acgagtgagt aatgtaggtg tcaagagcaa ttaatgtttt
    35341 ttgttgaatt aaatttgcat acgcatctca acgacaacac caataatttc acaatcccca
    35401 ttgatgggga tcagtggata gcgtgggttt agtggcttta ggaaacattg cccagcgtct
    35461 tctatgtact gtttaagggt tgcctctccg gagtttttga gcctggcgac gacatatttt
    35521 cctgaaaaaa cttctttgtc agggttaaca agaatgttca tgccttcaaa aatagagaac
    35581 tctccattct gtgatgtcat tgagtcccct ttcacttcca gccagaagcc gtgatcgccc
    35641 gcatatatat cgctaccaat ccattcttct ggataagcta tgtaatcttc gcagttccag
    35701 gggccagcac tcacccatgt tagtttcgga tatctataac ggtttggaga aactggctta
    35761 ggatttcgga cgttttgaat gctatctgta ggtgatgggg cgctgccgtc aagaagccat
    35821 ttaggagagc atttcaatgc atccgcgatt ctaaataggt ttgaagatct tgtgtcttgt
    35881 gagtctccta actctatttt gctgattgtt acacgcgaga caccggtcaa ttttgccagg
    35941 tcatcttgtg acattcctag ctgtactctg cgcattaaca gtctgccacc aaagctatcg
    36001 tgatccatct tctccccctt cttgtcgaaa taatcgtttc gaaacaaata tagcaaaaaa
    36061 tcttgaaatc taggtttcgg aaaattaaga ttaatcgaaa caaacgtgtc gaggtggata
    36121 tggatttgta tgaaattcta aaaaacatgt ttggatcgaa tgttgaaata ggtcgttatt
    36181 ttccccgaag gggaagggct agaaccggac aggctgtagg taagtggaaa acccgaggtg
    36241 tgccggaaga cgtggcgatc ctttgtcatc tggatcccaa aattccttat cagcatccgt
    36301 cgctcatgaa tgccagtcat gaatcgtgat gttctgccgg aggctgtatg tcccctgatt
    36361 acgttcagat ggagatgcct gccaggtaca gccaggcaga ttcagagtgg attaagcggc
    36421 agttacagag cctgccttcc tcactgagac agaaggtcgc cctgaaatac gcggaggttt
    36481 acgaagttac ttttgattcc gagcctgttt cattccgtaa ggagaacaga gcgaggcatg
    36541 aagccaatgt acggctgcgc agattcgttg aaacacacgg gcgtgcatta caggggtata
    36601 cgactcagcc gcccctggca ggaacgcaac agcgtacctg aatggtgttg gtcttaaagg
    36661 tgaccgacgg gctgagaccc cacttctcag gatgcactta tgtactacca agctagtaca
    36721 taagttgaga ggggggggta agggggggag tgcccgtgtg ttagtgcgaa gcactggaac
    36781 gggatcttcc aacagacggg tacagaggtt aggtagatct cgatctaaag gggggcaccc
    36841 ctgaaaaaac ggtcgtatca gcaaactagt acatcatggt taaaatatga gcgctgaact
    36901 gaaactggta ctaatcaacc ttctggaaga ggaattcgga catggaatgg acaaggtcac
    36961 attcgactac gttctgcaaa agaaaatcaa atccctgggc tgtgagttgc aacgctgttt
    37021 cactgtcagg ctgaaaggtg atcgcagggg atttatcgat cttctggtta tttctcccga
    37081 tggtcagcgt tgcgcagttg aggtggataa ccgatcgccc cgtcagcgtt cactgatgaa
    37141 aattcgagcg cttcctgaag gcatttcggg atttgttttt ctccgtgatg gcaaacatcc
    37201 acagcgttat atcgccgatg gtgtggatgt catccgggcg acccggttta agtaatcact
    37261 gtatcagccc tgtcatcggg agaataccat gctgaatatc aagccgaatt ttgctcagga
    37321 acgtgcgctg aatatgttgc gtcatgactg gaaaacatat cagtccttca tgatgtacat
    37381 gcctacaggg agtggtaaaa ccggcctggc ggcgttcatc actgctggtc tggtaagccg
    37441 tggcatgcgc gttctgtttg tcgttcctta taccattttg atcaaccaga cagcacagcg
    37501 ctttgttcag tacgggcttt ccggggatga aatcagcttt atctggcgtg atcatcccaa
    37561 taacgacccg tcacgactga ttcagattgc cagtgcggat acgctgattc gccgtgattt
    37621 tcctcagaat atcgacctgc tgattattga tgaagcgcat ctccgtaagc gccgcatcct
    37681 cacggaaatc gaacgactgg ttgcagaggg aaatgtgaag gtgattgggt tgtccggtac
    37741 acccttttca tcgttcctgg ggcgttatta ccagcgtctt ctcaagccga ccactatagg
    37801 ggaactgatc cagcgtggcg aactgagcaa ttacgaattt tatgcgccca cgaaaccgga
    37861 tctggaaggt gtaaaaacga aagtctcaat ggagtacggc agggattacg acgaatcaca
    37921 actggcagag attatgtgcg gcgcggatct ggttggcgat attgtcgata actggctgtg
    37981 taacggaagg gatttaccga cagtggcgtt ctgcgtcaat aaaacgcatg ccagcttcgt
    38041 gaccatgcag ttcaacaaag ccggaattaa cgcagaggtg atggttgctg aaacgccaca
    38101 tgatgagcgt cagttaatga tcagtcgttt tgaaacggga gcgacgaaaa tcattgtcag
    38161 cgttggcgtt ctggtggcgg gatttgacag tgatgtccgg tgcatcattt acgcacgccc
    38221 gacaaaatca gagattcgct ggcttcaggc gattgggaga ggattgcgta cagcgccagg
    38281 caaggagtca tgcctgattt tcgaccattc cggtacggtt catcgcctgg gatttcctga
    38341 aaatattgag tatgacgaac tgcccggcaa aaacgacggg atgcaggtgt catcatcacg
    38401 tggtaacgaa attcgggaag aaaaactgcc gaaagaatgt ccggggtgtc atttcatgaa
    38461 accggcaggt gtttatgtct gtccgaaatg cggatttaaa cctctggctg gtgaggatgt
    38521 gaacacagat acccgccgca atatcaggaa actcagcaag gacgaaaaaa tttacaccaa
    38581 aagcgacaaa caggcctggt ggagtcagat caaattctat cagcgtcagc gtgcctctct
    38641 ggggcgaccg gtcagcgatg gatggtgcgc tcacactttc cgggagaagt tcggcgaatg
    38701 gcctgacggg ctgagcagtt ttccgatgga aatcaccccg gaggtcagta attacatcag
    38761 acacaaattc atccggtttg ccagagggcg tagccgggca gagaagatga cggaaaaatc
    38821 ccccgaagcg ctttctctgt ccctgcttca tggtgtccgg tctggggtgc cggaaggcag
    38881 tgagtcgtgg cagatcatgc aggcaaagca acaacttcag aaaaacagaa acagtctgag
    38941 tcagtaagat gaaaacagca gaagcagcga aaggccactg gcctgaaatt ttaaaacatt
    39001 acggactgcc gccagtcacg ggtaaaaagc acttcaaagg ggaatgtccg gtttgtggtg
    39061 ccagaggtaa gttccgtatt gatgaccgtg atggcaccgg aacgtggatc tgtgtttgtg
    39121 gtagcggtga cggcatgaaa ctgatcgctc tgacacaaaa aagagcgttt catgaaattt
    39181 gcgctgaaat agaccgcatc acggggaatg aataccggag agataaaccg cctgtgatct
    39241 gtacgtcgga aagcctgaga tcgcgcgttc agcgtcgatt ttcagcgctc acggcgctac
    39301 agggaacatc tggtgctgaa tatcttcgtt cccgtggcat attcagtctt ccggttgagg
    39361 gagttcgctt caacagccag caaaattata acgggcgcat gttccagtct ctttatgccc
    39421 tggcgaccga tgacaaggga gaactgtgtt acctgcatca gacattgctt gatggagcta
    39481 aaaaagcaga cattggtatc agcgccagaa ggctcaaatc tttacaggac gataactatc
    39541 tggaccatgc ccggtcagtt gctatcagga tgtttccggt agccagtaca ctgggtatag
    39601 ctgaaggtat cgaaaccgcg ttgtccgcac atcagattta caaagtaaat acctgggcaa
    39661 ccattaacag tggttttatg aaaaagttcc gcgtaccggc gggagtcacc catctgatta
    39721 tttttgccga tcgcgatgaa tacagtgcta ccggaatggc tgcggcctgt gaatgcgcac
    39781 atgcaaatct ccaggcccga aatgacattc agcgggtaag tgtccggtgg cctgatcatg
    39841 atgatttcaa caatatgctc atgaacggcg atcaggttcg ggaactggtt ttttacagaa
    39901 aaaaggcggt tgcgtgatgc gtacggacaa tcaggtacat aaagctttat tcactatccc
    39961 gacggcagcg tacagcgccg ttccggcaaa tatcaaacct ctgccagaac aaaggaggat
    40021 caccggacat aaacagactg atgcttatct ctggattctg gaggttattc acctgaacga
    40081 ggccgtacat ctggacgcag ccgaagcagc actggagaaa ctcaaaataa cgccggaaga
    40141 agcgagtgaa cggtatggac gttatttgca ggagattaat gttgatcctt tccagattgc
    40201 tttcgcaacc ataggcatgg ataacccggc gcaggcgatc aggaatgccc gtgagaatat
    40261 caaaaaatcc tcatcagtca gggccacatt tggcagctat gaggcagcac tcgacgatgt
    40321 ggaggctgag cggataattc gcacctcccc gaaatttatc gacgattact actggggctg
    40381 gactgcggca gagaaaaaag ccggaagtat tgacggtgtt cgctcaaatg aaattgatga
    40441 tcagcgtcgt gcatatgttg atggctatcg tgatgtactg ccagagcctc atacattgtc
    40501 agacgttgtt cgtgagttta tttactggga ctggctttat gagatgcgcc agacagcggg
    40561 gagagaaaca ggggacaaat acggtttcac aggtgaacat catgagtcag tatatgatcg
    40621 gcagttctgg ctcgaaaatt tgctgggaaa aataaagcct gtgacgcgtg atgaagcggt
    40681 tgaagtgtgt cgctggtttc tggcaagcgg aaaagatgag tacatggaag acaatggttc
    40741 agcggtcatt ctcaatctgg ttggggagtg tgagcaatga agccggagat atccagtaga
    40801 cattttcccc ctgagcattc aaatcagaaa aagtatggtt acagcggagt acagaataat
    40861 gcgtgatatc caacaggttc tgtcccgatg gggtgcatgg gtggtaaata atcatgagag
    40921 tgtggcatgg tccggcgtcg ctgccggatt taagggagtg atcccatcaa aggttaaatc
    40981 acggcctcag tgtaccgatg atgatgcgct gattatcagc aattgcatgg cgcaactgaa
    41041 cgtcaacaac agcgatttgc atgattttct gtatgattat tatgtgttcg ggatgacgct
    41101 tatgggactg ggccgtaagc atgagcgctc tgattgctgg gccgggcggg tactgcaaaa
    41161 agctgaaggt gttattgagg ggatgttgat tatgcaggga atagaactgg aaatggacag
    41221 atacgttgag cgtgaacgat caggggcaca ggtcagtcag ttttccggac atacgggaaa
    41281 ttgaaagcgc ggggttttac tgtagaatga ctgcgggtgc ttgagggtgt ctgcctcggg
    41341 catgctgccg taaggcagac ggagaaaagc cccagttaac attacgtgtc ctgcaagacg
    41401 cttaacatta atctgaggcc caatctatgt cacacaaccg taggttagcc tcttacgtgc
    41461 cggaaggcaa ggagaagcag tctatgaagc agcaaaaggc gatgttaatt cgccctgatc
    41521 gtcatctgtt taactgtcat agtgacggca ctggtaacga ggaaagacct ctgcgaggta
    41581 cggatccgaa ccggccagac ggaggtcgct gtcttcgtag actacgaatc tgagaagtaa
    41641 gagaccaggc gggggagtaa tctcccgcca cctctgatgt gtcaggcatc ctcaacgcac
    41701 ccgtacttta tttgtcatat gctccgcaaa atgcaggttg tcgttgcaac ccgtgcggta
    41761 attttttgtg tgcgagtgct caaaaaacgt tgattttcat aaaaggatga ttttatgctg
    41821 cc
//
LOCUS       DAZGTJ010000033        41251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000033 DAZGTJ010000000
VERSION     DAZGTJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.033"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(246..322)
                     /locus_tag="ACHK8E_002234"
     tRNA            complement(246..322)
                     /locus_tag="ACHK8E_002234"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Met,seq:cat)
     gene            531..1628
                     /gene="mltA"
                     /locus_tag="ACHK8E_002235"
     CDS             531..1628
                     /gene="mltA"
                     /locus_tag="ACHK8E_002235"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP0344500.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1705..2511
                     /gene="tcdA"
                     /locus_tag="ACHK8E_002236"
     CDS             1705..2511
                     /gene="tcdA"
                     /locus_tag="ACHK8E_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP0344501.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2562..3005)
                     /gene="csdE"
                     /locus_tag="ACHK8E_002237"
     CDS             complement(2562..3005)
                     /gene="csdE"
                     /locus_tag="ACHK8E_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP0344502.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(3005..4210)
                     /gene="csdA"
                     /locus_tag="ACHK8E_002238"
     CDS             complement(3005..4210)
                     /gene="csdA"
                     /locus_tag="ACHK8E_002238"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP0344503.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTPQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4402..4629
                     /gene="ygdI"
                     /locus_tag="ACHK8E_002239"
     CDS             4402..4629
                     /gene="ygdI"
                     /locus_tag="ACHK8E_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP0344504.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4980..5897
                     /gene="gcvA"
                     /locus_tag="ACHK8E_002240"
     CDS             4980..5897
                     /gene="gcvA"
                     /locus_tag="ACHK8E_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP0344505.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5916..6311
                     /gene="ygdD"
                     /locus_tag="ACHK8E_002241"
     CDS             5916..6311
                     /gene="ygdD"
                     /locus_tag="ACHK8E_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP0344506.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6304..7404
                     /gene="rlmM"
                     /locus_tag="ACHK8E_002242"
     CDS             6304..7404
                     /gene="rlmM"
                     /locus_tag="ACHK8E_002242"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP0344507.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7448..8179)
                     /gene="fucR"
                     /locus_tag="ACHK8E_002243"
     CDS             complement(7448..8179)
                     /gene="fucR"
                     /locus_tag="ACHK8E_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP0344508.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(8237..8659)
                     /gene="fucU"
                     /locus_tag="ACHK8E_002244"
     CDS             complement(8237..8659)
                     /gene="fucU"
                     /locus_tag="ACHK8E_002244"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP0344509.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8661..10079)
                     /gene="fucK"
                     /locus_tag="ACHK8E_002245"
     CDS             complement(8661..10079)
                     /gene="fucK"
                     /locus_tag="ACHK8E_002245"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0344510.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10188..11963)
                     /gene="fucI"
                     /locus_tag="ACHK8E_002246"
     CDS             complement(10188..11963)
                     /gene="fucI"
                     /locus_tag="ACHK8E_002246"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP0344511.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11996..13312)
                     /gene="fucP"
                     /locus_tag="ACHK8E_002247"
     CDS             complement(11996..13312)
                     /gene="fucP"
                     /locus_tag="ACHK8E_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0344512.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13859..14506
                     /gene="fucA"
                     /locus_tag="ACHK8E_002248"
     CDS             13859..14506
                     /gene="fucA"
                     /locus_tag="ACHK8E_002248"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP0344513.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14534..15682
                     /gene="fucO"
                     /locus_tag="ACHK8E_002249"
     CDS             14534..15682
                     /gene="fucO"
                     /locus_tag="ACHK8E_002249"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0344514.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15737..16492)
                     /gene="xni"
                     /locus_tag="ACHK8E_002250"
     CDS             complement(15737..16492)
                     /gene="xni"
                     /locus_tag="ACHK8E_002250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP0344515.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16603..17970)
                     /gene="sdaB"
                     /locus_tag="ACHK8E_002251"
     CDS             complement(16603..17970)
                     /gene="sdaB"
                     /locus_tag="ACHK8E_002251"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP0344516.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(18028..19317)
                     /gene="sdaC"
                     /locus_tag="ACHK8E_002252"
     CDS             complement(18028..19317)
                     /gene="sdaC"
                     /locus_tag="ACHK8E_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP0344517.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19873..21237)
                     /gene="ppnN"
                     /locus_tag="ACHK8E_002253"
     CDS             complement(19873..21237)
                     /gene="ppnN"
                     /locus_tag="ACHK8E_002253"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP0344518.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21349..22197)
                     /gene="queF"
                     /locus_tag="ACHK8E_002254"
     CDS             complement(21349..22197)
                     /gene="queF"
                     /locus_tag="ACHK8E_002254"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP0344519.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22265..22810
                     /gene="syd"
                     /locus_tag="ACHK8E_002255"
     CDS             22265..22810
                     /gene="syd"
                     /locus_tag="ACHK8E_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP0344520.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23432..23761
                     /gene="yqcC"
                     /locus_tag="ACHK8E_002256"
     CDS             23432..23761
                     /gene="yqcC"
                     /locus_tag="ACHK8E_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP0344521.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23761..24543
                     /gene="truC"
                     /locus_tag="ACHK8E_002257"
     CDS             23761..24543
                     /gene="truC"
                     /locus_tag="ACHK8E_002257"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP0344522.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24561..25010
                     /gene="yqcA"
                     /locus_tag="ACHK8E_002258"
     CDS             24561..25010
                     /gene="yqcA"
                     /locus_tag="ACHK8E_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP0344523.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            25445..26797
                     /gene="gudP"
                     /locus_tag="ACHK8E_002259"
     CDS             25445..26797
                     /gene="gudP"
                     /locus_tag="ACHK8E_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP0344524.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26799..28139
                     /gene="gudX"
                     /locus_tag="ACHK8E_002260"
     CDS             26799..28139
                     /gene="gudX"
                     /locus_tag="ACHK8E_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP0344525.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28160..29500
                     /gene="gudD"
                     /locus_tag="ACHK8E_002261"
     CDS             28160..29500
                     /gene="gudD"
                     /locus_tag="ACHK8E_002261"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP0344526.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29724..32480)
                     /gene="barA"
                     /locus_tag="ACHK8E_002262"
     CDS             complement(29724..32480)
                     /gene="barA"
                     /locus_tag="ACHK8E_002262"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP0344527.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32537..33838
                     /gene="rlmD"
                     /locus_tag="ACHK8E_002263"
     CDS             32537..33838
                     /gene="rlmD"
                     /locus_tag="ACHK8E_002263"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP0344528.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33886..36120
                     /gene="relA"
                     /locus_tag="ACHK8E_002264"
     CDS             33886..36120
                     /gene="relA"
                     /locus_tag="ACHK8E_002264"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP0344529.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36216..37007
                     /gene="mazG"
                     /locus_tag="ACHK8E_002265"
     CDS             36216..37007
                     /gene="mazG"
                     /locus_tag="ACHK8E_002265"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP0344530.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37235..38872
                     /gene="pyrG"
                     /locus_tag="ACHK8E_002266"
     CDS             37235..38872
                     /gene="pyrG"
                     /locus_tag="ACHK8E_002266"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP0344531.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            38960..40258
                     /gene="eno"
                     /locus_tag="ACHK8E_002267"
     CDS             38960..40258
                     /gene="eno"
                     /locus_tag="ACHK8E_002267"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP0344532.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40318..41187)
                     /gene="ygcG"
                     /locus_tag="ACHK8E_002268"
     CDS             complement(40318..41187)
                     /gene="ygcG"
                     /locus_tag="ACHK8E_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0344533.1"
                     /translation="MRYFILIFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     TRKLESIFNNTQVQIAVLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     SANPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGG
                     GGSSGGGGASGRW"
BASE COUNT        10240 a         9975 c        10932 g        10104 t
ORIGIN      
        1 tataagcgta gcgccctgtt gtgatattga aatgttaaca taaccaaaat aaattgattt
       61 atagaatata taaaattcat taatggggta tatgtttaat atataaataa gatggatggc
      121 tttgatggag ggatattaac ggtttactca ttgtctcagg cttcactgaa acgggtaagg
      181 aagcgtaaaa acgaaaaaac cgcaagccaa tagtagcttt gcggtttgtc agcattctaa
      241 taaattggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
      301 taccaggctg ctccaccccg cgtccgtgga tgcgcactat actctgctcg cgttttcatg
      361 caaccttttt tttaccccga tcacggattc ggcatgattt tgaacaaaaa caaatcacta
      421 aagcctattt tttgtgcaaa atttgcgtca gggagaggct gctcatttca ttaaacgcgg
      481 tggtttgtta tcttcgttgc gccttatttt ttaacctgaa gaagagaaca atgaaaggac
      541 gttgggtaaa gtaccttctt atgggcacgg ttgtggcaat gcttgccgcc tgctcttcca
      601 aaccaaccga tcgcggacag caatataaag acgggaaatt tacccagcct ttctctctgg
      661 tgaaccagcc agatgccgtt ggcgcgccga ttaatgccgg tgattttgcc gagcaaatta
      721 accatatccg taattcgtca ccgcgtctgt atggcaacca gagtaatgtt tataacgcgg
      781 tgcaagagtg gctgcgcgca ggcggtgata cccgcaatat gcgccagttc ggcattgatg
      841 cctggcagat ggaaggtgtc gacaactatg gtaacgtgca gtttacaggc tattacacgc
      901 cggtaattca ggcgcgccat acccgtcagg gcgagttcca gtatccgatt tatcgtatgc
      961 cgccaaaacg tggtcgtctg ccgtctcgtg cggagatcta cgcaggagcg ctgagtgata
     1021 aatatattct cgcttacagt aactccctga tggataactt cattatggat gtgcagggta
     1081 gcgggtatat cgactttggt gatggcagtc cgcttaactt tttcagctat gcagggaaaa
     1141 acggtcatgc ctatcgcagc attggtaagg tgttgatcga ccgtggcgaa gtgaaaaaag
     1201 aagatatgtc gatgcaggcg attcgtcact ggggcgaaac acacagtgaa gcggaggttc
     1261 gcgagctgct ggaacagaac ccgtctttcg tcttctttaa accgcaatct tttgcaccgg
     1321 tgaaaggggc aagtgcggtg ccgctggttg gccgtgcttc tgttgcctcc gatcgttcaa
     1381 ttattccgcc aggtactacc ttgctggcag aagtgccgct gctggataat aacggcaaat
     1441 ttaacggtca gtacgaattg cgtctgatgg tggcgctgga tgtcggtggc gcaatcaaag
     1501 gccaacactt cgatatctat caagggatcg ggccggaagc cggacaccgc gcaggttggt
     1561 acaaccacta tggacgtgtc tgggtgctga aaaccgcccc gggcgcaggt aacgtcttta
     1621 gcggctgatg tgatattctg tatgccgttt ttacggatat gacagggtga ggggtaacct
     1681 cacccttttt aattctgagg tgctatgtct gtggtaatta gtgatgcatg gcgtcagcgt
     1741 tttggtggca cagcgcgtct gtatggtgaa aaagcattgc aactgtttgc tgacgcgcat
     1801 atttgtgtgg ttggtatcgg tggtgtcggt tcctgggcgg cggaagcgct agcgcgcacg
     1861 gggattggcg caatcacgct tatcgatatg gatgatgtgt gcgtcaccaa taccaatcgg
     1921 caaattcacg ctctgcgcga taacgtcggg ctggcaaaag cggaagtaat ggcggagcgt
     1981 attcgccaga ttaacccgga gtgccgtgta acagtagtgg atgacttcgt gacgccggat
     2041 aacgtagcgc aatatatgaa tgccggttat tcgtacgtga ttgatgctat tgatagtgta
     2101 cggcccaaag cggcgctgat tgcttattgt cggcgcaata aaatcccgct ggtcacgacc
     2161 ggtggcgctg gtgggcagat tgacccgacg cagattcagg ttaccgatct ggcgaaaacg
     2221 attcaggacc cgttggcggc gaagttgcgc gagcgcctga aaagcgattt tggcgtagtg
     2281 aaaaacagta aaggtaagct cggcgtggat tgcgtgttct ctaccgaagc gctggtgtac
     2341 ccgcagtcag acggcaccgt ctgtgcgatg aaagccacgg cggaagggcc aaagcggatg
     2401 gattgtgcct caggatttgg cgcggcgacg atggtgaccg ccacttttgg ctttgttgcg
     2461 gtttctcatg cgctgaagaa gatgatggcg aaagcggcgc gtcaggggta aacaatcagg
     2521 tcggatgcga cgcgaacgtc ttatccgacc tggttctgtg cttaaacctg cttcgccgca
     2581 gcgataatcg cctcgcttaa cgcattcaac ccctggctgc gtgaggcgct aagttgcgca
     2641 cgtaatccca gctcatcaaa caatgccagc ggcgactgtg cctgcaattc ggcggcggtt
     2701 ttcccctcaa cggcagtcaa caacaccgcc aacaggccgc gcacaatgcg cccttcgctg
     2761 tcgccaaaga aatgcatctt gccgttttca gccactgtat atcccagcca gacgcggttt
     2821 tcgcatccgg caatctcttt agcctgcgct tttaactcct ctggcaaagc cggaagctgt
     2881 ttccccagca tgatcaactg gcgatattta tcttcccatt gcgtcaacgg tgcgaaggta
     2941 ttgcgtaacg tttctgcggt tacggttgtg ccgaacggat gtccggcgaa ttgcgggttt
     3001 gtcattaatc caccaataat tccagcgcgc ggtcaacggc attcaccagc gcatccacat
     3061 cactctttgt attatatggc gcaaatgagg cgcgcagtgt gccggttacg cctaattctg
     3121 ccagtagcgg ctgagcgcaa tgctgcccgg cccgcagggc aataccgtac tccgccagca
     3181 gcgtcaccat atcgctgtga tgaacgccag caaaatcaaa ggccagcagg ctggaatcct
     3241 ggcagcgaaa tgagcggaag ccaggacgtt ttgccagcgc atcttccgcc agcgttgcta
     3301 agctacggct ccagctttcg gcctggttga tatcgtaatc tgccagccat tccagcgccg
     3361 cgcttaatcc tatgacacca gcgacatttg gcgttccagc ttccagtttc cacggcgcag
     3421 attgaggcgt gaagccgtca aaactcactt cgtgaaccat tttgccgccg cccagccagg
     3481 gcgacatcgc ttccagcagt tctgatttgc catacagcac gccgatacct gtcgggccat
     3541 acagtttgtg acctgaaaaa gcatagaaat caatatccag ttgctgaaca tccgcgggga
     3601 aatgcaccgc cccctgagca ccatcaacca tcaccaccat cccggctgaa tgagcaaagg
     3661 taatcgctcg cgccagatcc gggcaaccgc cagtgacgtt cgacatttgt cccagagcaa
     3721 gaattcgact acgaggtgtg atcagttctg gcaacaaatc gacatccggc agtcgctgcg
     3781 cattaagcgg caatttcacc actttggctc cagtttgttg ggcgaccatc agccagggga
     3841 cgaggttggc gtggtgttct gccacgctga caataatctc atcgcccggt tgcagatgcg
     3901 gacgcgcata gcattgcgcc accatgttga tggattcagt ggtgccgcgc gtccagacga
     3961 tagttttgtc atctggtgca ttcagtaatt gcgccacttt ctcccgtgca gcttcataac
     4021 gcgcggtcag gcgttgggct tcggcaaact ggctgcgatg gacgtttccg gcgctcaggc
     4081 tgtaaaactg tcgggtggct tcaaccacgg cttcaggttt aagcgtggtc gcggcgctgt
     4141 cgagatagac gcccgcatcc tgtagcgcgg gaaactgggc gcgaaactgc gcgggattaa
     4201 aaacgttcat ggtactcctc ggcttgatgc accgatcgtg acgcaaaatt cactttgata
     4261 caaggagagt gctaaccatc agaattatct ggatatcctg gcgaagccgc gtcagtaaca
     4321 tatgctgaat agtgttaggc gttaatttta gcctgttaat caatactatt aagatttatt
     4381 aatcgcttta aggagaagat gatgaaaaag actgccgcaa ttatttctgc ctgtatgctg
     4441 acttttgccc tgagcgcctg ttccggttcg aactatgtga tgcacaccaa tgacggacgt
     4501 accatcgtct ctgacggcaa accacagact gataacgata ccggtatgat ttcgtataaa
     4561 gacgctaatg gcaacaaaca gcagatcaac cgtaccgacg tgaaagagat ggtcgaactg
     4621 gatcagtaat tcgcgatcgc aaggtaaaaa aaagcaccgc aattaggcgg tgctacatta
     4681 atcactatgg acagacaggg taaatgtaca ggaagtgaaa aaaggtagct ttgctaccat
     4741 ggtctgaatc gcagaccaat tgcaaacaca acaacacaac atcacaaccg taagccaaaa
     4801 gttcaccaga acacgcattc cgataaaact tttcgttccg gctcaggaag tgccgccact
     4861 ataggtattt gctggtagaa gctcaacgga caatttataa tggctcagat taaaaaaact
     4921 aataggttac atagtgtaat ctaattgtta aattcattta acatcaaagt ttaaaagcca
     4981 tgtctaaacg attaccaccg ctaaatgcct tacgagtttt tgatgccgca gcacgccatt
     5041 taagtttcac tcgcgcagca gaagagcttt ttgtgaccca ggccgcagta agtcatcaaa
     5101 tcaagtctct tgaggatttt ctggggctaa aactgttccg ccgccgtaat cgttcactcc
     5161 tgctgaccga ggaagggcaa agctatttcc tcgatatcaa agagatattt tcgcaattaa
     5221 ccgaagcgac gcgtaaactc caggcccgta gcgctaaggg ggcgttgacg gtcagtttac
     5281 tccccagttt cgccattcat tggttggttc cgcgactttc cagctttaat tcagcttatc
     5341 cgggaattga cgttcgaatc caggcggttg atcgtcagga agataagctg gcggatgatg
     5401 ttgatgtggc gatattttat ggtcggggca actggccggg gctacgggtg gaaaaacttt
     5461 acgccgaata tttattgccg gtgtgttcgc cgctactgct gactggcgaa aaacccttga
     5521 agaccccgga agatctggct aaacatacgt tattacatga tgcgtcgcgt cgtgactggc
     5581 agacatatac ccgacagttg gggttaaatc atatcaacgt tcagcaaggg ccaattttta
     5641 gccatagcgc catggtgctg caagcggcta tccacgggca gggagtggcg ctggcaaata
     5701 acgtgatggc gcaatctgaa attgaggccg gacgtcttgt ttgcccgttt aatgatgttc
     5761 tggtcagtaa aaacgctttt tatctggttt gtcatgacag ccaggcagaa ctgggtaaaa
     5821 tagccgcctt tcgccaatgg atcctggcga aagccgctgc tgaacaagaa aaattccgct
     5881 ttcgttatga acaataattt acgtagggta cgaccatgac cagccgtttt atgctgattt
     5941 tcgccgccat tagcggcttc atttttgtag ctctgggcgc ttttggcgcg catgtgttaa
     6001 gtaaaaccat gggggccgtt gagatgggct ggatccagac cggcctcgaa taccaggcgt
     6061 ttcatacgct ggcgatctta ggtctggcgg tggcaatgca gcgtcgcatc agtatctggt
     6121 tttactggag tagcgttttc ctcgcgttag gcacggtgct gttcagcggc agcctttatt
     6181 gcctggcgct gtcccatctg cgtttgtggg cgtttgtcac accggttggc ggcgtgagct
     6241 tccttgcggg ctgggcgtta atgttagttg gtgctatccg tttaaagcgc aagggcgtaa
     6301 gtcatgaata aggttgtatt gctgtgccgt ccgggctttg aaaaagagtg cgccgcagaa
     6361 attaccgata aagccggcca gcgggaaatt ttcggttttg cccgcgtgaa agagaatgcg
     6421 ggttatgtca tttatgaatg ttatcaacct gatgatggcg ataagttaat ccgtgagctg
     6481 ccgttcagtt cattaatttt tgcccgccag tggtttgtgg tgggggaact cctgcagcat
     6541 ttgccgccag aagatcgtat tacccccatt gtcggcatgt tacagggtgt agtagagaag
     6601 ggcggtgaac tgcgtgttga agttgccgat accaacgaaa gcaaagagtt gctgaaattc
     6661 tgccgtaaat tcaccgtgcc gctacgcgct gccttgcgcg atgcgggtgt gctggcgaac
     6721 tatgaaacgc cgaagcgtcc ggttgtgcat gtattcttta ttgcaccagg ctgctgctat
     6781 accggttact catacagcaa caataattcg ccgttctata tgggcattcc gcgcctgaaa
     6841 tttccggcag atgcgccgag tcgttccacg ctcaaactgg aagaggcatt tcatgtgttt
     6901 attcctgcgg atgagtggga tgaacgcctg gcgaacggga tgtgggcggt ggatttaggc
     6961 gcttgcccag gcggctggac ctaccaactg gtgaagcgca acatgtgggt ttattccgtc
     7021 gacaacggcc cgatggcgca aagtctgatg gataccggac aggtgacgtg gctgcgggaa
     7081 gacggtttca aattccgtcc gacacgcagc aatatctcct ggatggtatg cgatatggtt
     7141 gaaaaaccgg cgaaagttgc ggcattgatg gcgcagtggc tggttaatgg ctggtgccgt
     7201 gaaaccattt tcaacctcaa actgccgatg aaaaaacgct acgaagaagt gtcacacaat
     7261 ctggcgtata ttcaggcaca gcttgatgaa catggcataa atgctcagat tcaggcgcgg
     7321 cagttgtatc acgatcgcga agaagtgacg gtgcacgtcc gccgtatctg ggctgcggtg
     7381 ggtggtcgtc gcgacgagcg ataacaggca aggccggggc gtcgtttccg gcctgctatc
     7441 ctttccgtca ggctgttacc aaagaagctg caacctggcg ctcatcagaa ataatgtgcg
     7501 tgacctcatc cagatgcccg atgcgggctt cccctgaacg attaaattta cttttatcaa
     7561 tcaataacaa cgattgcgcg gcacgtttta atagcatcga tttgtaatca gcgttgatgg
     7621 cattggagtc ccacagtgcg ccgctgctat cgatcccttc acaagaaaaa ataaacagat
     7681 cgatttccag cgatttaagc tgggaaatca gcgagggatt gacgtaacaa ccatatttgc
     7741 gctcaagtgt gccgccggaa ctgatcagtt gaatgcgttc gcgtttaccg agttcatggc
     7801 aaatcggatg gctattggtg aagacctgga tgttgatgtc aggcaactgg cgtgccagat
     7861 accagcaggt tgaacttgcg tctaaggcta ttaccatccc ttcttcaatc cacgcgagcg
     7921 cctcgcgcgc gatatctgct ttatgcgcat aatggctttt cagccggatg tgaaagggat
     7981 cgccactgtc ttgattttga cggtggatat atttagcgcg cccatgattg cgcagaattt
     8041 taccctgcgt ctgtaattca ttgagatcgc gacgaatggt ttctttactg acctttagct
     8101 gttcagagag agcttccgtg gtcaggctgg tatggttcag cagcaggtcg actatcgctt
     8161 gctggcgtgc cgctttcata tttttcaccc catcattcga taaccgcccg cgtcatgcgg
     8221 gcgtaccggt atgagattac ggtgttaccc ctttttttaa aagaatattc ccgtacttcg
     8281 ctcgttcgcc tgtgataacg atcgcaaagg ctttttgtgc ccgttcataa aacgcaaaac
     8341 gattgatgcg gatgatgtca ggacaggggg cttgtagtga aagcgcatta cgataacgtc
     8401 gttctacttc aggatctaga gtgtcacctt ctaccgccgc catcatcacc agcggtggtg
     8461 cataactgtc cagctcaaat aacgggataa tcgcctggag caagtcgctc accaacaggc
     8521 catcagcgcg gatcacctgc ggtcccatcg aatgggcggg aaagtgagca tcggaaaaaa
     8581 taatttcatc tccatgcccc atctctgcca gcactttcaa tagttcggga gaaattaacg
     8641 gcgaaattgt tttcagcatt tcacacctcc tctataaatt caggttcagt ttgcgggtag
     8701 aaataacggt actgataatg aatctgcgcg cgggcttctt ccggactgtt aaattcccct
     8761 acgccatacc aaccaaacag cgcagctcct gcgacggtcg tttcggcatc gtcgagaact
     8821 tttaccggaa tatcaagcat attggcttta atctgattcc acaatgtgtt gcgacttcct
     8881 ccgccgacta acaataattc agaggcttta aagtgcccga ttttttccag catctgtaga
     8941 ttgcgctgta attgcgtggt taacccttcc agcgccgcgc gatagaaatg cccccgcgtg
     9001 gtattaagcg ttacgccttg ccagccagcg ttctggcacg acaataaatc acactgcatt
     9061 ttcactccat ctgcgccagg cgcgatcagg cgagcttctt caatcaatat ttgccaggga
     9121 gtttcagctg tccagaacag ttttctcacc cattccagca cgccggatgc cagccattgc
     9181 atacctgggt tatacaaccc tgcctggcta tccagttcgc aagtagaacc ggcgtactga
     9241 cttaacagcg aagtatcaac ctgggcgctg cggaccatta aaatttccca tgtaccggaa
     9301 gagagcacgg gttcattttg ttcggcacca gcgccaaaaa gggcgaactg ggtatcgtga
     9361 cctgcggaaa tcaccggtat gccaacgggt aagccgagca ttgctgcggc gctgttctgt
     9421 agcgtaccaa tctgttcacc cgcttccacc agacgaggga agagtcggcg tggaataccg
     9481 gtggcttgta aaatttgcgg actgaaatcg cgttgctgga tatccagcat ctggctggtt
     9541 ccggccatcg taatatcagt agtgaattcg ccggttaaac ggtggttaat cagtgacgaa
     9601 ataaagagcc aggcgtgcgc gcgttccagc agttgtggat gattttcttt caaccacacc
     9661 aacttatata acgtattgaa actaaaggct ccgactccag aaatagcctg caactgctgt
     9721 gcggagatca accgttcaat attgtccatt accgcggctg ttcgcggaca tttccagcta
     9781 atgatcggat agagcagatt gccttgctta tctaccagag cgccatccac accaaaggtg
     9841 gtgacggcga taccgcggat atggcaatca gtcagttcac tattgatttg ccgacagcaa
     9901 tcagcaaagc gttgcaaaat ggcgtctaaa gaccactggt gccaggtgtt gttttccatc
     9961 gcgatatcgc tggcattagg cgttgaggcg cgggcaacaa tttttccctg ccgattaacc
    10021 gcgatggccc tgacattggt cgcgccacag tcgagtacca ggataacttc ttgtttcata
    10081 atcgctcctg caatatagcc ggataacatt gcttatccgg ctaaccactc ttgcttgttc
    10141 aggcccggta gccggagcga ccgggcatca catcagggag tcatgtatta acgcttgtac
    10201 aacgggccgt agttctggca agcgcggtaa tcctggcctt caatatccat gccgtgcgcg
    10261 gcccaggcag aaggacgata cactttggtc tcttcaacgt tgtgcataca taccgggata
    10321 cgcagcatgg aggcaagagt gataaagtct gcgccaacgt ggccgatggt cagaactccg
    10381 tggttagcgc cccagttcgc cattaccgag tacacatccg taaacggccc tttaccggtg
    10441 aggcgcggtg caaaccaggt ggttggccag gttgagttgg tgcgtttgtt gaggatgtca
    10501 tgcacatcct tcggcaattc cacgctccag ccttccgcga tttgcagtac cggtcccagg
    10561 cctttgatga tgttgacacg agtcatggtg aacgggacgc cgccttcggt aaggaagcgg
    10621 gaagagtaac cgccgccacg gaagtattcg tggatcgccg ggcaccattc ggtagcggcg
    10681 aggcaagcgt cagcctcttg ctgagagatt tcccagtgcg gcttcatcgt cggtttacct
    10741 tcgctgtcgc gttgtttaca ggagccgtcc agcgcagcag aaccggagtt gatcaaatgg
    10801 atgatgccgt gttctgccag cccgtccagt ttatgccccg ttacacgctc aattgcttct
    10861 ggtgaccagt aggtacgcac atcggcaaat atctgagcgg tgccggtgag ctggtggccc
    10921 attaacattg ccacgccgtt aagactgtcg ttttcggtcg ccacgacaaa cggtttgcgc
    10981 acgccattcc agtcaaatga actgttgagg atcgcttcag cggtatcgcc attgggatat
    11041 tgatcggtcc agtgacgttg cccctggaag cctgcagcga tggcgttgta gccaagtgat
    11101 tcttccacgc ggccaatatc ggccagtttg ctgttgcctt gcatcatgtc gcggatacac
    11161 atcgccatca gtaaactttc gcgcagaact gcgcggcttt gctcggcatt gcgttgatac
    11221 tgtttgttat tttcatcttc gccatagcgg aagtttttat cagcccaggc cagcgccatt
    11281 tccagttcgg cttcgtcgta aatcttctga tcgatacggc gacgcagttc ggtcatatcc
    11341 accgcctgga ctttcattcc cagccaggat tcaaagaagt tgtgatcaac gatggaaccg
    11401 gcgataccca tcgaaacgcc gcccagcgac agatagcttt tacctttcat gctggcgact
    11461 gctaaaccgg cgcgggcaaa gcgcagcagt ttttcttcaa catcggcagg aatcgatgtg
    11521 tcatcggcat cctgaacgtc atgaccgtaa atggagaatg ccgggatgcc tttctggctg
    11581 tgagctgcca gagccgctgc caggtaaaca gcgccgggac gttcagtgcc gttaaagccc
    11641 caaatggctt tcgggcgggt tggatccata tcgatggttt cactgccata gcaccagcaa
    11701 ggggttaccg taatggtgag gcctacattc tgactgctga atttttcttc gcaagcagcg
    11761 gcttcagcca tacccgcgat acaggtatcg gaaatgacac actcgacggc agctccacag
    11821 gcatggcgca gtttctcggt cagcagtgca gccgtggctt tcgccatatt cattgtttgt
    11881 tcttcaagcg actcacgaac acccatgcga cgaccgtcaa taaccgggcg gataccaatt
    11941 ttcggtaagc tgattttttt cattacagat tcctcacttt attcggaaaa tatgttcagt
    12001 tagttgctgt ttgagaacgg aaacgggcaa agataaagat gaccgcgaag cagagtgcgg
    12061 ggatcagttc agcagtgggg atgttgcccg ccgcgtcact gacaaaaccc atgaccggag
    12121 tgacaatacc gccgccaatg atggtcataa cgatgaagga cgaaccgtat ttggtgtcct
    12181 ggccgagatt cttaatgccc agcgagaaga ttgttgggta ctgaatcgac ataaaggcgc
    12241 tgcataaagt cagggctatt aagcccacat gaccgccagc gaaggctgag atcaggcaca
    12301 gtgccatagc gattaatgcg taggctgcca ggactttgtg tggtgcgaag cgactgatga
    12361 gccaggtacc ggtgaaacga ccaataaaga agcacaccat ggttccggtt aaatagttag
    12421 cggcaaagcc tgcggtcata cctggaattt cttctacagc gtagcgaatc aaatagctcc
    12481 agcaggccgt ttgcgcgccg acatagcaga attgcgctaa taccgcccag cgccagtgac
    12541 gaatacgcgc cagacgagaa agcgatgcgg agaacgatcc ttgtttggcg tcactgtgat
    12601 tatcactctg caatgccggg aatttcgtca gcatgatcag cagggcgacc agtaacacga
    12661 tagccacgat gatcatataa ggtgtctgta ccgataatac caggctgtgt ttatacgcac
    12721 tcaattgctc tggagacatt ttatcgagaa cgtcttgcga ttgatgtggc acgttagaca
    12781 aaataagact ttgtccaaag acaaccgcga taattgcgcc aaacgagtta aatgtttgcg
    12841 caagatttaa gcggaagtga ccactgcttt ccggccctaa taccgtaaca aaagggtttg
    12901 cggcagtttc cagacaacct aatccagctg caataataaa taggccaact aaaaataagg
    12961 tgtagttcat tatttctgca gcgggccaga ataatgcagc gcccaaggca tataaaaata
    13021 atccggtaat aatccctgct ttataactga gttttttcat caatatccca gcagggattg
    13081 ggataatgaa ataaccaaag taaaaggccg attggatcag gccagcctgg aaatttgtca
    13141 gcgtaaaagc ctgctggaat tgaggtaata aaatgtcgtt aaggttattg gctaccgccc
    13201 aaagaaaaaa cagtgagcac agcagcgcga atggaataat gtaacttctg ctttgccctg
    13261 catctttatc taccgcacgg taactctgcg tttgtattga tgtgtttccc atagcatcct
    13321 cttaggttca gaagcttaag cacgtaatcg gttatgtttt caaaagtgca aaaccagcga
    13381 gttcgggctt catatcagtg aatatttgcc tgtcagaaat gaactcatgg attcattttc
    13441 aaataaaaaa cgctctgtaa tatgacggcg gtcacactta ttagttggga ggtgacttta
    13501 tgtgactacc atcactttaa taatcgaaat ttcatgcccg tttcagaaaa taaatcataa
    13561 aaaacacaaa cccgatgacc gtattaattg tttgtgccga aaacgggcat ttctggcgag
    13621 ggggaatgga aaaatgaccg ttttgataaa tatttttaag agcggtcatt tttaataggg
    13681 ttataaaaag agaacttgca gttatttact gtgattagtt gaaccaggtc acaaaatata
    13741 agcttgtgaa tttctttctg gttaatcagg aagaagatgg tgggtaatta aacggctaat
    13801 tcaatagtgt gaaaggaaca acattattgc cctgttttga atcagagaga ggtagctaat
    13861 ggaacgaaat aaacttgctc gtcagattat tgacacttgc ctggaaatga cccgcctggg
    13921 actgaaccag gggacagcgg gtaacgtcag tgtacgttat caggatggga tgctgattac
    13981 gcctacaggc attccatatg aaaaactgac ggagtcgcat attgtcttta ttgatggcaa
    14041 cggcaaacat gaggaaggaa aactcccctc aagcgagtgg cgtttccata tggcggccta
    14101 tcaaagcaga ccggatgcca acgcggttgt tcacaatcat gccgttcatt gtacggcagt
    14161 ttccattctt aaccgaccga tccccgcgat tcactacatg attgcggcgg ctggcggtaa
    14221 ttctattcct tgcgcgcctt atgcgacctt tggaacacgc gaactttctg aacatgttgc
    14281 gctggctctc aaaaatcgta aggcaacttt gttacaacat catgggctta tcgcttgtga
    14341 ggcgaatctg gagaaagcgt tatggctggc gcatgaagtt gaagtgctgg cacaacttta
    14401 cctgacgacc ctggcgatta cggacccggt gccagtgctg agcgatgaag agattgccgt
    14461 agtgctggag aaattcaaaa cctacgggtt acggattgaa gagtaatttc gtaaagcaac
    14521 aaggagaagg atgatggcta acagaatgat tctgaacgaa acggcatggt ttggtcgggg
    14581 ggctgttggg tctttaaccg atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt
    14641 caccgataaa acgctggtgc aatgcggcgt ggtggcaaaa gtgaccgata agatggatgc
    14701 tgcagggctg gcatgggcga tttacgacgg cgtagtgccg aacccaacaa ttactgtcgt
    14761 caaagaaggg ctcggtgtat tccagaatag cggcgcggat tacctgatcg ctattggtgg
    14821 cggttctcca caggatactt gtaaagcgat tggcattatc agcaacaacc cggagtttgc
    14881 cgatgtgcgt agcctggaag ggctttcccc gaccaataaa cccagtgtac cgattctggc
    14941 aatccccacc acagcaggta ctgcggcaga agtgaccatt aactacgtga tcactgacga
    15001 agaaaaacgg cgcaagtttg tttgcgttga tccgcatgat atcccgcagg tggcgtttat
    15061 tgacgctgac atgatggatg gtatgcctcc agcgctgaaa gctgcgacgg gtgtcgatgc
    15121 gctcactcat gctattgagg ggtatattac ccgtggcgcg tgggcgctaa ccgatgcgct
    15181 gcacattaaa gcgattgaaa tcattgctgg ggcgctgcga ggatcggttg ctggtgataa
    15241 ggatgccgga gaagaaatgg cgctcgggca gtatgttgcg ggtatgggat tctcgaatgt
    15301 tgggttaggg ttggtgcatg gtatggcgca tccactgggc gcgttttaca acacgccaca
    15361 cggtgttgcg aacgccatcc tgttaccgca tgttatgcgt tataacgctg actttaccgg
    15421 tgagaagtac cgcgatatcg cacgcgttat gggcgtgaaa gtggaaggta tgagcctgga
    15481 agaggcgcgt aatgccgctg ttgaagcggt gtttgctctc aaccgtgatg tcggtattcc
    15541 gccacattta cgtgatgttg gtgtacgtaa ggaagatatt ccgacactgg cgcaggcggc
    15601 actggatgat gtttgtaccg gtggcaaccc gcgtgaagca acgcttgagg atattgtaga
    15661 gctttaccat accgcctggt aaatgcgctg atgtgataat gccggatgcg acgtttgcgc
    15721 cgtatccggc acgccgttac cttaccaacc gcaattgctg taaattgccg tcgatatgca
    15781 aatcggtttg taagcgggca atatcgcggc acagaaacgc catctcttta tgggtttcta
    15841 attttttgcg ccacttttcg gcaaccgcat tcagattctc atatatccct tccagactct
    15901 taaactcgac cagcagctgc gtggcgcttt ttggtccgat ccccacaaca cccggtacct
    15961 ttgaactgct gatccccgca agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa
    16021 attctttatc gataaatggc gcatccagcc aacgtttctg gaagtaatca cgaatacgta
    16081 atgtcggtga aagtaactga cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc
    16141 cggcctgtgt cactttgacc gccagcgtgg cggctaagtc atcggcttcg ttgccgctgg
    16201 cggaccagca cgggacgccg cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt
    16261 gcaactcttc cggcattggc ggtcgacccg ctttgtaatc tggtaaacgc tgatgacgcc
    16321 agccgctact gcggttttca tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca
    16381 taatgagctg atcgagcgcg tgctggcagg tttcgacaca gggcgaaccc tgaacggcat
    16441 gaatgcgacg aataagattc agtgcatcga caataagcaa atgaacagcc acgataaccc
    16501 ccttaaagat caggcgataa gggtaacatt cgatgccgga acaggctatg gctggttgag
    16561 aaaacgggaa gaggcctcgc aaaacgaggc ctccgggaga gattaatcgc aggcaacgat
    16621 cttcattgcc aggccaccgc gagaggtttc gcggtacttg gcgttcatat ctttacctgt
    16681 ttcgtacatg gtctcgataa ctttatcgag gcagacgcgc ggctcgctgg tacggcgcag
    16741 cgccatacgc gcggcgttca ccgcttttac tgccgcaatg gcgttacgct cgatgcacgg
    16801 tacctgcacc tgtccagcaa ccgggtcaca cgtcagaccg aggttatgct ccatggcgat
    16861 ttccgccgcg atgcacacct gcgccgggct tgcgcctaac agttctgcca gaccagctgc
    16921 cgccatagag cacgccacgc caacttcacc ctggcaaccc acttcggcac cagaaatcga
    16981 cgcgttcatc ttataaagag aaccaatggc gctggctacc agcagataac gagccagtga
    17041 gttagcgttc acttcgcgga taaacttatc gtagtacgcc agcacagccg ggataatccc
    17101 gcacgcaccg ttagtcggcg cagtcaccac gcgaccgcca gcagcattct cttcgttcac
    17161 tgccagtgca aacatgttga tccagtcaac aaccgccatc gggtcagtgg tggttttatc
    17221 ctggctgacc agcatccggc gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg
    17281 taacacgcct tcggtagaaa taccacgctc aataccgcca cgcatgactt cccagacgtt
    17341 cgccaggtgc tgttccagct cttctttgct gtgcagcgcc agctcgtttt tcatcatcag
    17401 gccagagagt gacagcccgg tttcctgaca atgtttttgc agatcggctg ctgaactgta
    17461 cggataagga acttcaaccg gtgcgctatc ctgctggcca aaatgttctt catcaacgat
    17521 aaagccaccg ccgatagagt agtaagtctg gctgtaaacg actttatcgc ccgccagtgc
    17581 ggtaatgcgc ataccgtttt catgcagaga aaggttgtcg gcatggaagt tcatgcactg
    17641 atcaaccggg aactccactt catgctgacc gtttgccagc atcaggcgac catgagtatt
    17701 cacatcctga ataaaaccag ggatggaatc gatatccacg gtatccggca ggttacccgc
    17761 caggcccata ataatggcga tatcagtgtg gtggccttta ccggtcagag agagcgagcc
    17821 gtacacgtca accaccacgc gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc
    17881 ggtaaattgt ttacccgctt tcattggtcc aacggtatga gaactggaag ggccaatgcc
    17941 gattttgaaa atatcgaata cgctaatcat aggaaataca tcgcgttaaa acggaggaag
    18001 cgccgcccga aagcggcgcg aaaggactta gctgaacaga gagtagaaga ttgcggagat
    18061 tgcaatcaga cccatcacga caacgaatac gttgctgatg tgaccgctgt acttacgcat
    18121 tgccggtact ttctgaattg cgtacatcgg catcaggaac aggatcatcg cgatgattgg
    18181 gccgcccagg gtttcaatca tacccaggat gctcgggttc agggtggcaa caatccaggt
    18241 cgttaccagc atgaacagcg cagtgatacg gttcagcttg ttgatttcga tagacttacc
    18301 tttaccacgc agagatttaa tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg
    18361 gccgaggaag gatttggtga tagcgataat cgcgataatc ggagccatcc acgcgatgat
    18421 cggcgcgtta aagtggttag ccaggtaaga cagaatcgag atgttctgct ctttagccgc
    18481 agccaggtct gctggagtca ggctcagtac acagctgaag acgaagaaca ttacggtcag
    18541 caccatcatg atgtgtgcga atgccaggat cttcgagcat ttctgttctg ccatatcgcc
    18601 gtactcttca cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt tgaacgagaa
    18661 caccattacc ggaattgcca gccacagggt catccaaaga ccgtttccgg ttgcagatgc
    18721 agtgtccaga gacagcgttt ccagtgctgc gccgttccac tgcgggatca ggtacagagc
    18781 cagcagcatc agtacgccaa caaacgggaa taccagaata ctcatcgctt taacgatcat
    18841 ctgctcaccg aagcgaacga tggtcatcat acccacgatc aggatcagcg acagaatcgc
    18901 acgcggcggt ggtgtcatac ccagctggtg agacatgaag ctttcaactg tattggtgat
    18961 tgccacgcta taaaccagca gaatcgggta gatagcgaag aagtagagca gggtaatcag
    19021 tttacctgcg ccaataccaa agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt
    19081 tttaccagac agtacgaagc gagtcagacc gcggtgagca aaaaatgtca tcgggaacgc
    19141 aaggatagcc atgatgatca gcgggatcat accgccaaca ccggcgttga ttggcaggaa
    19201 cagcacgccc gcgccgattg ccgtgccgta aaggcccagc atccacatgg tgtctgtctt
    19261 gcgccaggca ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta
    19321 tttctcctgg aggaatgcaa ataattcagc ttttagccag ttcagcccgg gaaggtgcct
    19381 gaccgtaaaa atgaaatctg tctataggcg atggcttaat catttaccgc cgtaataatt
    19441 tgagggcgga aagatacaca caagttatgt atctatcagt gatcttgatc ccaaatacaa
    19501 taatctactt ggaagataga ttatcccaac gctttactct gtaaatcaaa agtaaaagag
    19561 aatttaaggg caatatttgc ccattaaacg acatctgact tcttccgggg cgcgcaatat
    19621 atgtaggttg aactgaaaaa atcaatactt ttagttaatc atggcgataa tttctttttc
    19681 atagcgataa aatgtggttt atgaaaatag ggaaacgttt gcgttacaac ataaattgta
    19741 tgacatatta actgtaatga aatttatcgc atgagggtgg taagaaacat tcatataaca
    19801 aatgaaataa attgatgggt atgtgaccaa aaacgagaaa acccggcaac aaacgtcacc
    19861 gggggaggga gattacgtgc agatttcgta gcaagggatg taggctgagc ctggcaactt
    19921 catacgatgc tgggcaacaa aaccctgtag caggtcatcc atacgacgca taatctcttt
    19981 atcgccgttg attttataag gaccaaactc ttcaatggcg cgaataccga cttcttttac
    20041 gttacccgcc acaataccgg agaacgcacg gcgcaggtcg gcagccagca cttcaacggg
    20101 ttgatccggg taaagcttca gattagccat attctcgtga gacggctcaa acggcatttg
    20161 caaatctggc gcaatgcgca ttgaccagtt aaagctgtag gcatcgcctg tgtcgcggcg
    20221 attttctttc accagcggca tcgatttttt catctgacga gcgacttcag cggcgtcatc
    20281 aatgatgatg cggtaatggc ggcgcgcgtt ttcacccaat gtatgcacga caaactcgtc
    20341 cagtacgcgg aagtagtcgg cgctctcttt cgggccggtg aggatcaatg gtaaaacctg
    20401 atctttgttg gccgggttca ttaaaattcc cagcaaatac agcaactctt ctgccgtacc
    20461 cacaccgcca gggaagataa tgataccgtg agcgatacgg acaaacgctt ccagacgttt
    20521 ttcgatatcc ggcatgatga tcaattcgtt gaccagcggg ttaggcggtt cagcggcgat
    20581 aatcgacggc tctgtcatac caataaaacg actgtctttg taacgctgct gtgcgtgtcc
    20641 gaccgcagcg cctttcatcg gcgcttccat cgctcccgga ccacagccgg tgcagatatt
    20701 cagctcacgc aggcccagct gattcccgac gcgacgggca tacaaatact cgttttcgtt
    20761 aattgagtgc ccgccccagc agaccaccat atttggcgct tcaccgacat gcagtgcgcg
    20821 agcgttacgc aagatggaaa agaccaggtt agtgatgtgg acggagttgt cgagattcag
    20881 gttgggaaaa cgaacggtgt tatggatttg cccgtaaacg aagagaatgt cacgcagaac
    20941 cgcgaacaag ttggcctgca aagcgcgaat aattcgccca tcgacaaaag cctcttccgg
    21001 gggatttatc agttccagct ttacgccgcg ttcacggcgc aggacgttaa tatcgaaatt
    21061 ttcaaaacga gacagcaatt ctttgctgtt atcggtcaaa ctaccggagt tcagtacggc
    21121 aagtgaacag ttgcgaaaca gttgatagag gtcgctgctg gcggtgcgtt taagcatatc
    21181 cacttccagc tgcgacaaca tatccatgga gccaagcggg ctaatatgtg taatcaagaa
    21241 aaactcctta tgggacgaat atcgtcattc cctttgatta caatagccct gcctgatgac
    21301 ctttcacaac cttctgcgtg aatccagcac gcaaaattga gaaaaaattt attgccgaac
    21361 cagtcttgtg gtcgatggga caaaatcgct attactgcgc cacgggttaa tgtccagacc
    21421 gccacgacgg gtataacgtg cgtaaacgct caatttctct ggctggcaga agcgtaacag
    21481 gtcattaaag atgcgttcca cgcactgttc gtggaactcg ttgtgatgac ggaaagaaac
    21541 caggtaacgc agcagttttt ctctgtcaat ttggcgtcca cgatactgaa tttggatcga
    21601 tccccaatct ggttgatggg tgatcaggca atttgatttc agcaggtggc tgacgagcgt
    21661 ctcttctact actttttcgc cactggtggc attctccaga tagtcagtag tgaattcata
    21721 gttatcgata gtgatatcct ggtcatcaat gcaagtgcca ttgaaatgac ctatcggttg
    21781 gccttccagt tcatcaagac gatataacgc cacgctaacc ttaccctgag cgcaagtgct
    21841 taagtcgcgc tccagcgtct ggcgcacctc atcccagtta ttaaaacgcg tctggttaaa
    21901 actgttgaga tagagcttaa aactcttcga ctcaatcaga tttgcgctgg tgtaatcaag
    21961 ttcaacatga ccgacagcga cctgcggcaa acctttcgca ttcagccagg aaagttcata
    22021 cagcgtccag atatccgtac cgtgaaaagg caggttatcc gctttcagac ccagcgggtc
    22081 gcgattcagg ctgcgtggaa cgccttgcag taggctggcg tcataggtat cccggtaatc
    22141 ggttgatttt ccaagagtca ggcccgcaag tgcctgatgg tttgcataag aagacatgtt
    22201 tcaccgtcat aaaccagata aactaaacga caagtgtatc tggtttaagt gaagagagaa
    22261 attagtggac gatttgaccg cacaagccct gaaagatttt actgcacgct actgcgatgc
    22321 gtggcatgaa gagcataaaa gctggccgtt aagtgaggaa ctgtacggcg ttccttcacc
    22381 gtgcattatt tcaactaccg aagatgccgt atactggcaa ccccagccgt ttaccgggga
    22441 acaaaatgta aacgcggttg aacgcgcttt tgatattgtg atacaaccca cgattcatac
    22501 cttttatacg actcagtttg ccggggatat gcacgcgcag tttggcgata tcaaactgac
    22561 attgctgcaa acctggagtg aagacgattt ccgtcgggtg caggaaaatc tgattggtca
    22621 tctggtgact cagaaaaggc taaaactgcc gcccacgcta tttatcgcga cgctggaaga
    22681 agagttagag gtcatctcgg tgtgtaatct ctccggtgaa gtgtgcaaag agacgctagg
    22741 aacacgtaaa cggacgcatt tagcctcaaa tcttgcggaa ttccttaatc aacttaagcc
    22801 tcttctgtaa tccaaatacc ccatctggtt gtgagagatc tcttacagac tctgtaggag
    22861 atcgccagga aataagcgaa tacttaaaaa gataagaatc gttattctca tttaaaaaca
    22921 acatgttgct tgttaattct taactttcat atgaaatttt ccttaaggca ttgtctgtaa
    22981 gcgccttgta agacttcgcg aaaaagacga ttctatcttc gtcgacaggg agtcagacaa
    23041 cgaagtgaac atcaggatga tgacacttct gaaggacaca ccaggatggt gtttcaggga
    23101 aaggcttctg gatgaagcga agaggatgac gcaggacgcg ttaaaggaca cctccaggat
    23161 ggagaatgag aaccggtcag gatgattcgg tgggtcagga aggccaggaa cacttcagga
    23221 tgaagtatca catcggggtg gtgtgagcag gaagcaatag ttcaggatga acgattggcc
    23281 gcaaggccag aggaaaagtt gtcaaggatg agcagggagc aacaaaagta gccggaatgc
    23341 tgcgaaacga accgggagca ctgtgaatac agtgctccct ttttttattc ctgctatcct
    23401 tcgcggcagt ttttcttatt gaggttgctt tatgaccact catgaccgcg ttcgtctcca
    23461 gttgcaggcg cttgaagcgt tactgcgtga acatcagcac tggcgaaacg ataaacccca
    23521 gccgcatcaa tttaacagta cccaaccgtt ctttatggac accatggaac cactggagtg
    23581 gttgcagtgg gtgctgatcc cgcgaatgca cgatttgtta gataacaacc agccgttacc
    23641 gggcgctttt gccgttgcac cctattatga aatggcgctg gcgactgatc atccgcaacg
    23701 cgcgcttatt ctggctgagc tggaaaaact ggatgctctt tttgcggatg atgcgagcta
    23761 atgctggaaa tactctatca ggatgaatgg ctggttgcgg taaataaacc ctccggctgg
    23821 ctggttcacc gcagctggct ggatcgcgac gagaaagtag tggtcatgca aaccgtgcgt
    23881 gacctgatag gccagcatgt gtttactgct catcgtctgg accgacccac ttctggtgtg
    23941 ttgttgatgg gattatccag cgaggccgga cggctgctgg cacaacagtt tgaacagcac
    24001 caaatccaga aacgttacca tgcgattgtg cgcggctggt tgatggaaga agcggtgctg
    24061 gattatccac tggtggaaga actggacaaa atcgctgata aatttgcccg cgaagataaa
    24121 ggcccgcagc cagcagtgac gcattatcgc ggtctggcga ccgtagaaat gctggtagcg
    24181 accggacgtt acccgaccac gcgctacggc ctggtggaac tggagccgaa aaccggacgt
    24241 aagcaccagc tccgtcgcca tcttgcccat ttgcgccacc cgattattgg cgacagcaaa
    24301 catggtgatt tacgccagaa tcgcagcggt gctgaacatt ttggccttca gcggttaatg
    24361 ctgcatgcca gtcagttgtc attgacgcat ccttttactg gcgagccact gactattcac
    24421 gcgggcctgg acgacacctg gatgcaggcg ttatcacaat ttggctggcg gggtctgctt
    24481 cctgaaaatg aaagggttga gttcagtgcg ccatcgggtc aggatggaga gataagttcc
    24541 taatccaggg agaaaacggt atggcggaaa ttggtatttt tgtcggcact atgtacggaa
    24601 attcgctgtt agtggcggaa gaagcggaag caattctgac cgcgcagggc cacaaagcaa
    24661 cggtatttga agatcctgaa ttaagcgact ggctgcccta tcaggataag tatgttctgg
    24721 tggttacgtc cacgaccggg cagggcgacc ttcctgatag cattgtgccg ctctttcagg
    24781 gaattaaaga taccctgggc ttccagccga atctgcgtta tggcgtgatt gcgctcggcg
    24841 acagtagtta tgtgaatttc tgcaatagtg gcaaacagtt cgatgccttg ttgcaggaac
    24901 agagcgctca gcgggttggc gaaatgctgt tgattgacgc cagtgagaac ccggaaccgg
    24961 aaacggaatc aaatccgtgg gtcgaacatt ggggcacgat gttgtcctga tatgttcagc
    25021 gagcggtaaa tgccgtttta gcggtgctga atcgaatctt ttcaggcaaa tgccagtaaa
    25081 aactgcttca tagcgcggat ttttactggc gtttgcctgg agtcaagcga tccatttcat
    25141 actcttcttt atttcttcgt tttaaccctt cctttcttgt tcttgttttc atttccgtga
    25201 agtggattcc accgtccagg gctaatgcca aaatcgggcc tcattgaacg cattaatgtt
    25261 gtgttgttgc acggtgagcc gctatggcgc gctttttata ctgctattgc cagatataaa
    25321 cacgcgccgt attcggcgaa cgacctataa aaacggcaaa aaacacccta cgtcacctct
    25381 gatttcctgg cgatgtcgca gtccagagtg agcgtggcta acgcgaattt tcaggagtgc
    25441 aacaatgagt tctttaagtc aggctgcgag cagtgtggaa aaacgcacga atgctcgtta
    25501 ctggatagtg gtgatgttgt ttatcgtcac atccttcaac tacggcgacc gcgctacgct
    25561 ttctattgcc ggttcggaaa tggccaaaga tatcggcctt gatcccgtgg gaatgggcta
    25621 tgtgttctct gccttctcat gggcgtatgt tatcgggcag atccctggtg gctggttgct
    25681 ggatcgtttt ggttcaaaac gcgtctactt ctggtcgatc tttatctggt cgatgtttac
    25741 cttgctgcaa ggcttcgtcg atatctttag tggattcggc attatcgttg ccctgtttac
    25801 actgcgcttc ctggtcgggc ttgctgaagc gccatctttc cctggcaaca gccgcattgt
    25861 tgcggcctgg tttccggcgc aggaaagggg aacggcggtg tcgattttta actccgccca
    25921 atacttcgca acggttatct tcgcgccgat tatgggctgg ctaactcatg aagtgggctg
    25981 gtcacacgtc ttcttcttta tgggcggtct gggcattgtc atcagcttta tctggttgaa
    26041 agtcatccac gagccaaatc aacatccggg cgtaaatcag aaagagctgg agtacatcgc
    26101 cgcgggtggc gcgctgatca atatggatca gcaaaacacc aaagttaaag tgccgttcag
    26161 cgtgaagtgg gggcagatca aacagctgct cgggtcacgg atgatgatcg gcgtttatat
    26221 cggtcagtac tgtatcaacg ccctgactta ctttttcatt acctggttcc cggtttatct
    26281 ggtgcaggcg cgcgggatgt cgattctgaa agcgggcttt gtggcttccg ttccggcggt
    26341 ttgcggtttt atcggcggtg tgctgggtgg gattatttcc gactggctga tgcgtcgcac
    26401 gggttcactg aacattgcgc gtaaaacacc gatcgtgatg ggcatgttgt tgtcgatggt
    26461 gatggtgttc tgcaactacg tcaacgttga gtggatgatc atcggcttta tggcgctggc
    26521 cttcttcggt aaggggatcg gtgcgctggg ttgggctgta atggctgata ctgcgccaaa
    26581 agagatcagc ggtctttccg gtggcctgtt taacatgttt ggtaacattt ctggcatcgt
    26641 cacgccaatc gcaattggtt atatcgttgg cacgactggc tcctttaatg gggcgctgat
    26701 ttatgttggt gttcatgcct taatcgcggt gctcagctac ctggtgctgg tgggcgatat
    26761 caagcgtatt gagctgaaac ctgtcgcggg gcaataagat gacgacacaa tccagtcctg
    26821 ttattactga tatgaaagtc attccggtcg ccggacatga cagcatgttg cttaatattg
    26881 gtggtgcaca taacgcatat ttcacccgca atattgtggt actcaccgat aatgccgggc
    26941 ataccggcat tggtgaagcg ccgggcggag aggtgattta tcaaacactg gtcaaggcta
    27001 ttccgatggt gctgggccag gaagttgcgc gcctgaataa agtggttcag caggtgcata
    27061 aaggtaatca ggcagccgat tttgatacct tcggcaaagg tgcctggact tttgaattgc
    27121 gcgttaatgc cgtggcggcg ctggaagccg ccttgcttga cctgttaggt caggcgctga
    27181 atgttccggt ctgcgaactg ttagggccag gcaagcaacg tgatgctatt accgttctcg
    27241 gttatctgtt ttatatcggt gatcggacca aaaccgatct tccttatctg gaaaatacgc
    27301 cgggcaacca tgagtggtat cagttgcggc atcagaaagc gatgaacagc gaagccgttg
    27361 tgcgtctggc ggaagcctca caggatcgct atggctttaa agatttcaaa cttaagggtg
    27421 gcgtgttacc tggcgagcaa gaaatcgaca ctgttcgtgc attgaagaaa cgcttccctg
    27481 atgcgcggat taccgttgat cccaatggtg cctggctgct tgatgaagcc atttctctgt
    27541 gcaaagggct gaatgatgtc cttacctatg cggaagaccc gtgcggcgct gagcagggtt
    27601 tctccggtcg tgaagtgatg gcggagtttc gacgggcgac cggcttgccc gtcgcgacca
    27661 acatgatcgc caccaactgg cgcgaaatgg gtcatgcggt gatgctcaat gcggtagata
    27721 ttccacttgc cgatccgcac ttctggacgc tttccggtgc agtccgcgtg gcgcaacttt
    27781 gcgacgactg ggggctgacc tggggctgcc attccaataa ccactttgat atctctctgg
    27841 cgatgtttac ccatgtgggt gcggcggcac cgggtaatcc taccgctatc gatacccact
    27901 ggatttggca ggagggcgat tgtcgcctga ccaaaaatcc gctggagatt aaaaacggaa
    27961 aaattgccgt tcctgatgcg cccggtctgg gcgtgggact ggactgggag caggtacaaa
    28021 aggcgcatga ggcctataaa cgtctgcctg ggggtgcgcg taacgacgcg ggcccgatgc
    28081 agtacctgat ccccggctgg acctttgacc gtaaacgtcc cgttttcggc cgtcattgat
    28141 tttgaaaaag gacataaaga tgagttctca attttcgacg cctgttgtta ctgaaatgca
    28201 ggtcatcccg gtggcgggcc atgacagtat gctgatgaat ctgagtggtg cacacgcacc
    28261 gttcttcacg cgtaatattg tgattatcaa agataattct ggtcacaccg gggtagggga
    28321 aattcccggc ggcgagaaaa tccgtaaaac gctggaagat gcgattccgc tggtggtggg
    28381 taaaacgctg ggtgaataca aaaacgttct gacgctggtg cgtaatacat ttgccgatcg
    28441 tgatgccggc gggcgcggtt tgcagacatt tgacctgcgg accactattc atgtggttac
    28501 cgggatagaa gcggcaatgc tggatctgct ggggcagcat ctgggggtta acgtggcatc
    28561 gctgctgggc gatggtcaac agcgtagcga agtcgaaatg ctcggttatc tgttcttcgt
    28621 cggtaatcgc aaagccacac cgctgccgta tcaaagccag ccggatgata aatgtgactg
    28681 gtatcgcctg cgtcacgaag aagcgatgac gccggatgcg gtggtgcgcc tggcggaagc
    28741 ggcatatgaa aaatatggct tcaacgattt caaactgaag ggcggtgtac tggccgggga
    28801 agaagaagca gaatctattg tggcactggc gcaacgcttc ccacaggcgc gtattaccct
    28861 cgatcctaac ggtgcctggt cgctgaacga agcgattaaa attggtaaat acctgaaagg
    28921 ttcgctggct tatgcagaag atccgtgtgg tgcggagcaa ggtttctccg ggcgtgaagt
    28981 gatggcagag ttccgtcgcg cgactggcct gccgaccgca accaatatga tcgccaccga
    29041 ctggcggcag atgggccata cgctctcgtt acaatccgtt gatatcccgc tggcggatcc
    29101 gcatttctgg acgatgcaag gttcggtacg tgtggcgcaa atgtgccatg aatttggcct
    29161 gacctggggt tcacactcta acaaccactt cgatatttcc ctggcgatgt ttactcatgt
    29221 agccgctgct gcaccgggta aaattactgc tattgatacg cactggatct ggcaggaagg
    29281 caatcagcgt ctgaccaaag aaccgtttga gatcaaaggc gggctggtac aggtgccgca
    29341 aaaaccagga ttgggcgtag aaatcgatat ggatcaggtg atgaaagccc acgagctgta
    29401 tcagaaacat gggcttggtg cgcgagacga tgcgatggga atgcagtacc tgatccccgg
    29461 ctggacgttt gataacaagc gcccgtgcat ggtgcgttaa gacagaaaac caaaaccggc
    29521 tctatctgga gccggtttga gactgatgcc aaactgcctg ttgcgctacg tatcagggtt
    29581 acatcatttt ttaattcatt gaatttaatt ggttttgtag gccggataag gcgttaacgc
    29641 cgcatccggc ataaacacag gcacttttca ccaatctgaa accagcgtca taaaaatccg
    29701 gttgctactc gacaagacgt ccattacccg agaattttgc tggcttcgcg cgctacatta
    29761 tccatctcgt ccaacagttc cagcagctcc ggctctaaat cttcttcttt agtaccgcta
    29821 cgtagctgtt gttcgatcag ttggcagaga ttcttcatac gcggtacacc gctatagccg
    29881 caactgccat gcagtttatg aatcaaatca accaggcctt ccgggttttc tccaaccagc
    29941 tgttcctcaa ctttgttgcg aacttcaggc aggaaatcga gtaacatttg cagcatatcg
    30001 cgcgctaaat cggtttttcc tgctgcctgg cgtagtgcca gttgccagtc gagggtcgca
    30061 ttcgggttga ccacaatttc gttgacttcg ggcgtcacga cgcgagagga aataccgctg
    30121 ccaggcttgt agcgcaacag caaattatgc aatcgctctt cttcgatcgg tttggccaga
    30181 taatcactca tccccgcgcc aagcagtttc tctttttgcc cggccatcgc atgcgccgtt
    30241 accgcgataa ccggcgtttg ctgctgatgc gggagctggt ggatgagctc acatgcccga
    30301 atgccgtcca tgtccggcat ttgaatatcc attaagatta aatcgaacgg catctgtttc
    30361 gcccgttcaa ccgcctgatg cccgctatcg caaagttcca catgttgcac catatcttcc
    30421 agcaatgcgc cgataagttt caggttagcg gggttgtcat caaccgccat gactgtcatt
    30481 gccagcttac tttcatcggt tacaggcaaa agcgtgtttt gtttgtgatg acaaaattcc
    30541 gtcagggcag gcaacaggcg cgtaggtgtt aatggtttca gcagacacgc gccgatacca
    30601 tcttgcttaa gtttttcagc attgacttgt gcatggcaag gaagtgccag catcaggaaa
    30661 tcggtcatcg atactgcttt cgctaatcgc tcatgttgca ttgttagcgg ctcgcggaag
    30721 gtcaccgcta tgcctaataa catcatgtcg taatgcgcgg gaggcagcgc ggagaacgtt
    30781 gggctataaa ccacttccag cggcgtttca cttaaaatat ccagcgtgca ttgtgctgct
    30841 gcggagtttg gttcgacata ggccaggcgc ttaccagcga ggcactgggt ggatggccct
    30901 tcgataataa tgttcgggtt cagatcgaga ttaatgtgga accagaaagt tgaaccacga
    30961 ttcggcaggc tatggaacga aatatcgccg cccatttcat taaccagttt ttgtgtaatc
    31021 accagcccca gcccggtgcc accatgacgg cgggaaatac tggcatcagc ctgtctgaag
    31081 gcctggaata agcgcgattg atcgcgttca ggaataccaa tgccggtatc ccgaatctgc
    31141 acttcaatct gcactttggt attactcagc gcacgttttt ctaccagaat atcgatgttg
    31201 ccattctcgg tgaatttaat tgcattccct accaggttag tgatgatttg ctgtaatcgt
    31261 agtgggtcgc cgatcacgtt atcaggcacg tcgcttttaa tattgagcgt cagttccagc
    31321 cctttatcgt gagaagaatg tgccagcagg gtaacgactt catccagcgt gctgcgtagt
    31381 gggaatggaa tactttccag aatcagctta ccggcttcca gtttcgagaa gtcgagaaca
    31441 tcattaataa ttgccagcaa attatttgcc gaacgttcaa tcgtattcag gtgatcgcgc
    31501 tgcgttggtg ttaattctgt tttcagcgtc aggcgggtaa agccaataac accattcagt
    31561 ggtgtacgca gctcgtgtga catatttgcc agaaactcgg atttaatacg cgccgcttcc
    31621 tgggcgcgct ttttcgccag atccaactca acgttctgaa tctccatttg ctccagcgtc
    31681 tcacgcagat cggacgtcgc ctggtcgata ttgtgttgca tctcttcgtg ataagccgcc
    31741 agcgacattg ccatcgagtt gataccgttt ttcagcatat ccagctcgcc gagcataaat
    31801 ccttccaccc ggctgtcgag ttgcccgcga cggatgcggt cgacggtatt caccatgttg
    31861 cgaatcggac cggttacatc gcgcattaag cgccagccaa aaataagcgc aataccgata
    31921 caaaacagca tcatcacgct ggaaataaag atctctttat attgctgcaa gcgaaccgat
    31981 ttaagatcca gctccagcgc aatatatccc agcatatttt gactattttt ggcatcgcta
    32041 ctgggcgatt cgtcggggga gtaactttca gaaataatcg gcgtgcggag gatcataata
    32101 tcgccgtcac gagtgacagt gagctggcga ggaaacggca cgttgctgcc gagctgcatt
    32161 gatgagggat caagatgaaa attggaggtg acaaagagtc ggttattttc atcataaacc
    32221 gaaatcgcgc gaacaatatc ggaatggcga cgatgcagta cgcttattaa ctgaccgata
    32281 gattcgcgat tttgcaggct catgccatat tcagtagaaa ctgcaagcgg ctcaataatg
    32341 ctggctccgg catcttccag ttgacgctgc aagtcgttat agcgatgcac aacgaaaaag
    32401 atactcagca ataaaccaat aaggacggtc ggtgccagga tcagaatcat catgcgtgcg
    32461 cgcaggctgt agttggtcat ggagttccgt tatgggacaa ttaaggtcac actgttaaat
    32521 tgagaaagtc tcgccaatgg cgcaattcta ctctgcaaaa cgacgcacga cgacgcgtca
    32581 gatcataact gtttcagtca acgacctcga ctcttttggt cagggcgtgg cacgacataa
    32641 cggcaaagcg ttatttattc ctggattatt gccacaggaa aacgcggaag ttactgttac
    32701 tgaagataaa aaacaatacg cccgcgctaa agtcgtgcgc cggttaagcg atagcccgga
    32761 acgcgaaacg ccacgctgtc ctcattttgg cgtatgcggc ggctgtcagc aacaacacgc
    32821 cagcgtggat ttacagcagc gaagcaaaag tgcggcactc gcccgattaa tgaaacacga
    32881 agtctctgaa gtgatcgccg atgttccctg gggctatcgc cgtcgcgcgc gtttaagtct
    32941 gaactactta ccgaaaacac agcaacttca gatggggttt cgcaaagcgg gctccagtga
    33001 cattgtcgac gttaaacaat gtcccatttt agtgccccaa cttgaagcat tgctgcccaa
    33061 agtcagggca tgcctgggca gcttacaagc tatacgccat cttggtcatg ttgaactggt
    33121 acaggcaacc agcggcacgc tgatgatttt gcgccatacc gcaccgctaa gttcggctga
    33181 tcgcgaaaaa ctggaacgct tttcgcattc tgaaggcctg gatctgtatc tcgcccccga
    33241 tagtgagata ctcgaaaccg tctctggtga gatgccctgg tatgactcaa atgggttgcg
    33301 cttaactttt agcccgcgcg attttattca ggtcaatgcg ggtgtgaacc agaaaatggt
    33361 agcgcgtgcg ttggaatggc tggatgtgga acctgaagat cgcgtactgg atctgttctg
    33421 cggtatgggc aactttacac tgccattggc gacacaagct gctagtgtgg tgggtgtaga
    33481 aggtgttccg gcgctggtgg aaaaaggcca gcagaatgcg cgtcttaatg gcttacagaa
    33541 tgtgacgttt tatcacgaaa atcttgaaga agatgtcaca aagcagccgt gggcgaaaaa
    33601 cggcttcgat aaagtgttgt tggacccggc gcgagcaggt gccgcaggtg ttatgcagca
    33661 aattataaaa ctggaaccta ttcgtatagt ttatgtatcc tgtaaccctg caacgctggc
    33721 tcgggatagc gaagcgttat taaaagcagg atataccatt gcgcgactgg cgatgctgga
    33781 tatgttccca cacacgggac atctggaatc gatggtactt ttctcgcgcg ttaaatagtt
    33841 acgatttgcc gatttcggca ggtctggtcc ctaaaggaga ggacgatggt tgcggtaaga
    33901 agtgcacata tcaataaggc tggtgaattt gatccggaaa aatggatcgc aagtctgggt
    33961 attaccagcc agaagtcgtg tgagtgctta gccgaaacct gggcgtattg tctgcaacag
    34021 acgcaggggc atccggatgc cagtctgtta ttgtggcgtg gtgttgagat ggtggagatc
    34081 ctctcgacat taagtatgga cattgacacg ctgcgggcgg cgctgctgtt ccctctggct
    34141 gatgccaacg tagtcagcga agatgtgctg cgtgagagcg tcggtaagtc ggtcgttaac
    34201 cttattcacg gcgtgcgtga tatggcggcg atccgccagc tgaaagcgac gcacactgat
    34261 tctgtttcct ccgaacaggt cgataacgtt cgccggatgt tattggcgat ggtcgatgat
    34321 tttcgctgcg tggtcatcaa actggcggag cgtattgctc acctgcgcga agtaaaagat
    34381 gcgccggaag atgaacgcgt actggcggca aaagagtgca ccaatatcta cgcgccgttg
    34441 gcgaaccgtc ttgggattgg gcaactgaaa tgggagctgg aagattactg cttccgttat
    34501 ctccacccga ccgaatacaa acgcatcgca aaactgctgc atgaacgccg tctcgaccgc
    34561 gaacactata tcgaagagtt tgtcggccat ctgcgcgctg agatgaaagc tgaaggcgtt
    34621 aaagctgaag tgtatggccg tccgaaacac atctacagta tctggcgcaa aatgcagaaa
    34681 aagaacctcg ccttcgatga gctgtttgat gtgcgtgcgg tacgtattgt cgccgagcgt
    34741 ttacaggatt gttatgccgc actggggata gtgcacactc actatcgcca cctgccggat
    34801 gagtttgacg attacgtcgc taacccgaaa ccaaacggtt atcagtccat tcataccgtg
    34861 gttctggggc cgggtggcaa aaccgttgag atccaaatcc gcaccaaaca gatgcatgaa
    34921 gatgcagagt tgggtgttgc tgcgcactgg aaatataaag agggcgcggc tgctggcggc
    34981 gcacgttcgg gacatgaaga ccggattgcc tggctgcgta aactgattgc gtggcaggaa
    35041 gagatggctg attccggcga aatgctcgac gaagtacgta gtcaggtctt tgacgaccga
    35101 gtgtacgtct ttacgccgaa aggtgatgtc gttgatttgc ctgcgggatc aacgccgctg
    35161 gacttcgctt accacatcca cagtgatgtc ggacaccgct gcatcggggc aaaaattggc
    35221 gggcgcattg tgccgttcac ctaccagctg cagatgggcg accagattga aattatcacc
    35281 cagaaacagc cgaaccccag ccgtgactgg ttaaacccaa acctcggtta cgtcacaacc
    35341 agccgtgggc gttcgaaaat tcacgcctgg ttccgtaaac aggaccgtga caaaaacatt
    35401 ctggctgggc ggcaaattct tgacgacgag ctggaacatc tggggatcag cctgaaagaa
    35461 gcagaaaaac atctgctgcc gcgttacaac ttcaatgatg tcgacgagtt gctggcggcg
    35521 attggtggcg gggatatccg tctcaatcag atggtgaact tcctgcaatc gcaatttaat
    35581 aagccgagtg ccgaagagca ggacgccgcc gcgctgaaac agcttcagca aaaaagctac
    35641 acgccgcaaa accgcagtaa agataacggt cgtgtggtgg tagaaggggt cggtaacctg
    35701 atgcaccaca tcgcgcgctg ctgccagccg attcctggag atgagattgt cggcttcatt
    35761 acccaggggc gcggtatttc agtacaccgc gccgattgcg aacaattggc ggaactgcgc
    35821 tcccatgcgc cagaacgcat tgttgacgcg gtatggggcg agagctactc cgccggatat
    35881 tcgctggtgg tccgcgtggt ggctaatgat cgtagtgggt tgttacgtga tatcacgacc
    35941 attctcgcca acgagaaggt gaacgtactt ggcgttgcca gccgtagcga caccaaacag
    36001 caactggcga ccatcgacat gaccattgag atttacaacc tgcaagtgct ggggcgcgtg
    36061 ctgggtaaac tcaaccaggt gccggatgtt atcgacgcgc gtcggttgca cgggagttag
    36121 tcttacatag ctggatatgg cgtcagccgt atccagcgat atcttttact cgaaggtatt
    36181 acacctgacg gacccgcaat tttcaggaca ttctaatgaa tcaaatcgac cgtttgctca
    36241 ccatcatgca gcgcctgcgt gacccggaaa acggctgccc gtgggataaa gagcagacat
    36301 ttgccaccat tgcgccttac acccttgaag aaacctacga ggtgctggac gccatcgccc
    36361 gtgaagattt tgacgatctg cgcggtgaac tgggcgatct gctgttccag gtggtgtttt
    36421 acgcacaaat ggcccaggaa gaagggcgct ttgattttaa tgatatttgc gctgcgatta
    36481 gcgataaatt agaacgccgt catccacatg tttttgctga cggttctgcc ggaaacagcc
    36541 gcgaagttct tgctcgttgg gagcaaatca aaactgaaga gcgcgcgcaa aaagcgcagc
    36601 attcggcgct ggacgatatc cctagtagtt tacccgcctt aatgcgcgca caaaaaatcc
    36661 agaaacgttg cgccaacgtt ggcttcgact ggacgacgct tggcccggta gtcgataaag
    36721 tctacgaaga gatcgatgag gtgatgtacg aagcgcggca ggttgttgtc gaccaggcta
    36781 aactggagga ggaaatgggt gacctgctgt ttgccacagt caatctggct cgtcatttag
    36841 ggacaaaagc ggaaatcgca ttgcaaaaag cgaacgaaaa attcgagcgt cgttttcgcg
    36901 aagtggagcg tattgttgcc gcgcgtggac tggaaatgac aggtgttgac ctcgaaacaa
    36961 tggaagaagt ctggcaacag gtaaaacggc aggaaattga tctctaaggg aattacgtgg
    37021 tcaagcgcga tttgtgtcat tttttaaatg acaagcgctt gatttgcgtc aaaaacattt
    37081 accccaaagg ggctattttc tcactcctga tttcaatagt gcgctggcga agaggaggga
    37141 taatgaaagt ttgtggcaca ggtcatgttc gggtatactg ctttcccgtc ctggttattc
    37201 catcgtcttt tcaacctaac ttctcaggtt cagcatgaca acgaactata tttttgtgac
    37261 cggcggggtc gtatcctctc tgggtaaagg cattgccgca gcctccctcg cagccattct
    37321 tgaagcccgt ggcctcaatg tgaccatcat gaaactggat ccgtacatca acgtcgatcc
    37381 aggtactatg agcccaatcc aacacgggga agtgttcgtt actgaagacg gcgctgaaac
    37441 cgacctggac ctggggcact acgagcgttt cattcgtacc aaaatgagcc gccgcaacaa
    37501 cttcaccacg ggtcgtatct actctgacgt tctgcgtaaa gaacgccgcg gtgactacct
    37561 cggcgcaacc gtgcaggtta ttccgcacat cactaacgca atcaaagagc gcgtgctgga
    37621 aggcggcgaa ggtcatgacg tagtactggt cgaaatcggc ggtactgtag gtgatatcga
    37681 atccctgccg ttcctcgaag cgattcgcca gatggctgtt gaaattggcc gtgagcacac
    37741 tctgtttatg cacctgacgc tggtgccgta catggcagcg tctggtgaag tcaaaaccaa
    37801 accgactcag cactctgtaa aagagctgct ctccatcggt atccagcctg acatcctgat
    37861 ttgtcgttca gatcgcgccg ttccggcgaa cgaacgtgcg aagattgcat tgttctgtaa
    37921 tgttccggaa aaagcggtta tttctctgaa agacgtcgat tccatctata aaattccggg
    37981 cctgttgaaa tctcaggggc tggacgatta tatttgtaaa cgattcagct taaactgccc
    38041 ggaagcaaat ctgtccgaat gggaacaggt tatcttcgaa gaagcgaatc cggtaagtga
    38101 agtcaccatc ggtatggtcg gcaagtacat tgaactgccg gatgcttata aatcagtgat
    38161 cgaagcactg aaacacggtg ggctgaagaa tcgtgtcagc gtcaacatca aactgatcga
    38221 ttcacaagat gttgaaacgc gcggcgttga aatccttaaa ggtctggacg caatcctcgt
    38281 acctggcggt ttcggctatc gtggcgtaga aggcatgatt acgaccgcgc gttttgcgcg
    38341 tgagaacaat attccttatc tgggtatttg cctgggtatg caggtggcgt taattgatta
    38401 cgctcgccat gttgccaaca tggagaacgc caactctacg gaatttgtgc cagactgtaa
    38461 gtacccagtt gtggcgctga ttaccgagtg gcgcgatgaa aacggcaacg ttgaagttcg
    38521 tagcgagaag agcgatctcg gcggtaccat gcgtctcggc gcacagcagt gccagttggt
    38581 tgacgatagc ctggttcgcc agctgtacaa tgcgccgaca attgttgagc gtcatcgtca
    38641 ccgttacgaa gtcaacaaca tgctgttgaa acagattgaa gatgcaggtc tgcgcgttgc
    38701 gggccgttcc ggggatgatc agttggtcga gatcatcgaa gttccgaatc acccgtggtt
    38761 cgtggcttgc cagttccatc cggagtttac ttctactcca cgtgatggtc acccgctgtt
    38821 tgcaggcttt gtgaaagccg ccagcgagtt ccagaaacgt caggcgaagt aagtaaaaaa
    38881 gttagaacgg caacgcgtac cctgggtacg cgttgtttgt ctggagtttc agtttaacta
    38941 gtgacttgag gaaaacctaa tgtccaaaat cgtaaaaatc atcggtcgtg aaatcatcga
    39001 ctcccgtggt aacccgactg ttgaagccga agtacatctg gagggtggtt tcgtcggtat
    39061 ggcagctgct ccgtcaggtg cttctactgg ttcccgtgaa gctctggaac tgcgcgatgg
    39121 cgacaaatcc cgtttcctgg gtaaaggcgt aaccaaagct gttgctgcgg taaacggccc
    39181 gatcgctcag gcgctgattg gcaaagatgc taaagatcag gctggcattg acaagatcat
    39241 gatcgacctg gacggcaccg aaaacaaatc caaattcggc gcgaacgcaa tcctggctgt
    39301 atctctggct aacgccaaag ctgctgcagc tgctaaaggt atgccgctgt acgagcacat
    39361 cgctgaactg aacggcactc cgggcaaata ctccatgccg gttccgatga tgaacatcat
    39421 caacggtggt gagcacgctg acaacaacgt tgatatccag gaattcatga ttcagccggt
    39481 tggcgcgaaa actgtgaaag aagccatccg catgggttct gaagttttcc atcacctggc
    39541 aaaagttctg aaagcgaaag gcatgaacac tgcagttggt gacgaaggtg gctatgcgcc
    39601 gaacctgggt tccaacgctg aagctctggc tgttatcgct gaagctgtta aagctgctgg
    39661 ttatgaactg ggcaaagaca tcactctggc gatggactgc gcagcttctg aattctacaa
    39721 agacggtaaa tacgttctgg ctggcgaagg caacaaagcg ttcacctctg aagaattcac
    39781 tcacttcctg gaagaactga ccaaacagta tccgatcgta tctatcgaag acggtctgga
    39841 cgaatctgac tgggacggtt tcgcatacca gaccaaagtt ctgggcgaca aaatccagct
    39901 ggttggtgac gacctgttcg taaccaacac caagatcctg aaagaaggta tcgaaaaagg
    39961 tatcgctaac tccatcctga tcaaattcaa ccagatcggt tctctgaccg aaactctggc
    40021 tgcaatcaag atggcgaaag acgctggcta cactgcagtt atctctcacc gttctggcga
    40081 aactgaagac gctactatcg ctgacctggc tgttggtact gctgcaggcc agatcaaaac
    40141 tggttctatg agccgttctg accgtgttgc taaatacaac cagctgattc gtatcgaaga
    40201 agctctgggc gaaaaagcac cgtacaacgg tcgtaaagag atcaaaggcc aggcataagc
    40261 ctgactttat ctgatttaaa aatgccagcc ttcgggctgg cgttttttat ggttgagtta
    40321 ccagcggccg gatgcgccgc caccgccaga agaaccgcca ccgccgctaa acccgccgcc
    40381 tgagcctcca gcggaaccgc tgttgtctga atgataactt ctacctgatg ctcttttctg
    40441 aagtacacat aaacaggtaa agaccataaa tatggtgaaa gtaaaaaaga aggaaaatac
    40501 agcaatggaa aaagttttat cggagaacag gaataaatct aaaagataga ttgctgaaat
    40561 acagacactg cttttacatg cgcgacaaaa attactgcct tgatgaaaaa accagaaagg
    40621 gaagaacatc actgcaaata cccaaaaaat agcaaagtag taatgatcgc tggaagacgc
    40681 tgattcactt tctgaaggat tagccgaata ttggtgttgt gaagtgagtt ggttattcaa
    40741 agcgattata gctaactcta atcccttagc taatttttgt tgtttaaatg cgggtatcat
    40801 gttgctacgg atgatatctc cagccagagc atcggttacc ttttcttcca gcccataacc
    40861 tacctggatg cggacagtgc gatccgacca ggcaacaatg atcagtatcc catcattgcg
    40921 tttggcatct cctaaacgcc aattgttaaa aacccttgta gcatattgtt caattgtctc
    40981 gtctttggtt gtgggaataa ttaatacagc aatttgtact tgggtattat tgaaaataga
    41041 ttctaacttc cgcgtcagtt ctttcttttc ttgtgagttt aagctactcg taagatccgt
    41101 aacttgttgc tgtaattgtg ggacaatagt tggttgggct gcaacaaagg aacacacgaa
    41161 tgtgaatatt agtatgaaat agcgcattat aaatgtccca ttaaagctgg ataaataaat
    41221 tgacggcggc atcggtaatt tgagattcgt t
//
LOCUS       DAZGTJ010000034        40847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000034 DAZGTJ010000000
VERSION     DAZGTJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.034"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..92)
                     /locus_tag="ACHK8E_002269"
     CDS             complement(<1..92)
                     /locus_tag="ACHK8E_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344534.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFST"
     gene            complement(184..261)
                     /locus_tag="ACHK8E_002270"
     tRNA            complement(184..261)
                     /locus_tag="ACHK8E_002270"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Ile,seq:gat)
     gene            339..518
                     /locus_tag="ACHK8E_002271"
     CDS             339..518
                     /locus_tag="ACHK8E_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0344535.1"
                     /translation="MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKD
                     IPLPTLKSIKKQAGL"
     gene            535..933
                     /locus_tag="ACHK8E_002272"
     CDS             535..933
                     /locus_tag="ACHK8E_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0344536.1"
                     /translation="MLYPVAIDKSDSSFGVRVPDIPGCFSGGNNYQDAIESAHEAIEA
                     HIELLVENGEAVPKATCIENWLEDPDYSDAVWALVDVDITRLMGKAEKINVTLPSLLI
                     RRIDQFVAAHPEYGSRSGFLSRVAADKITG"
     gene            complement(1009..1704)
                     /locus_tag="ACHK8E_002273"
     CDS             complement(1009..1704)
                     /locus_tag="ACHK8E_002273"
                     /inference="COORDINATES: protein motif:HMM:NF018074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0344537.1"
                     /translation="MTPQQMVWAREQVIFALNPPDIRQKGQLEPFGDFARSDTKRYGR
                     QRKRTLNIDGRWYCRDSEPVFAAEPPRYSAQSEMITPEAFRTAKLRRKIYCLEDYQRA
                     WVLFSYSLQKKRIHHLLVSEFIWMRVRERLRGKRVTERMIGNLIRLTRIVALNAAVIA
                     GGVVAGAVLIAPSCAAQQIDIKPNTWSQHYKGYWCFMHEVCCELDHEALITIMGIKNH
                     QKKYKFAKVNILR"
     gene            complement(1706..2686)
                     /locus_tag="ACHK8E_002274"
     CDS             complement(1706..2686)
                     /locus_tag="ACHK8E_002274"
                     /inference="COORDINATES: protein motif:HMM:NF017914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0344538.1"
                     /translation="MIRDVPAVLAGMPSGEIPSSGQGLAGEEELKDFFADDRVIKRAG
                     GSDALARWVSRIAGCQCENGDHVENMMTARTMQGGAIRLCHACDNRHYMKGYRELADV
                     IARNRAEWLMDFIRMNLGLGEDHQVSIPELFCWAVLNDVADAVPVEIVRRIRRLPEEE
                     QLRGTMREADINPWQISADAVVKKLAERAKPVSTHNALVAHAVRREKTDEQERAVLKL
                     ATDSDPVMGYMRRPRMQRLVLPDYTAWVKRQRCCGCGKVADDPHHITGHGFGGAGIKA
                     HDLFVIPLCRECHQALHNDRKGWEERNGSQLLWVLRTLDRAAGIGALAGG"
     gene            complement(2796..3077)
                     /locus_tag="ACHK8E_002275"
     CDS             complement(2796..3077)
                     /locus_tag="ACHK8E_002275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344539.1"
                     /translation="MRWIMRGLELERMTPEKMHAIGMECIRRSEAMAQAGMTKDAVRK
                     NLLPAVQRLNRAKHELQLVVDQMIDKAAELEKYGSALEKIVQDIVGTEQ"
     gene            complement(3191..5113)
                     /locus_tag="ACHK8E_002276"
     CDS             complement(3191..5113)
                     /locus_tag="ACHK8E_002276"
                     /inference="COORDINATES: protein motif:HMM:NF017640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0344540.1"
                     /translation="MTVYSAGGPRMPEPEEELIFTEEVNSAFSGLPRFVSVKLGRRIS
                     SAWQTKGFAGAREKFALMVQKDLPCITGVNKRYAINPDELPGWLFGGLASDQAYGAVH
                     SMTWRFNSLVDGDDGDAHLLAQDLSAFLIAEMQHLSALLADEDADVAAGAIYAMVAGL
                     VEHFRMEPPDWKRFIGRKMTLDQLTVAISRMLSVRWWQGKLRVFSRRWREHLLIAMGS
                     VRRHSSPHCSYEWVQRWLAGRKHGRRLMAETDVEDEETGEKFSLLDLVDSSISGAEKR
                     RSELMTRVKGLEDLARFEGVAQEQGYVGLFFTMTAPSKYHAWLETGYRNRRYNGASPK
                     DTQNYFSGLWRQISATLRRRGIGVFGLRSAEPHHDGTPHWHGVLYVHQEYAVALQEIL
                     EYYANRDDDAEKVTIRKAGRKSHLEIKPVLDDLGSATAYIAKHIGMNIDGCASGGMNH
                     ETGHTLVVTARHASAWASLWGIKQFQFIGSAPVSVWRELRRFNNQVHADRISPALGEL
                     HRAASEGDWKTYTRLQGGPFVARSDLVLRTWYQRSEEPDECGRHASAIKGVYMPGCDT
                     PPVVTRSRNWRISRNVTTGSLGVNRWPALTPWTCVNNCISGRKRPADHPPDLCIRIPV
                     QLELNFWEEKKGKNEH"
     gene            complement(5192..5422)
                     /locus_tag="ACHK8E_002277"
     CDS             complement(5192..5422)
                     /locus_tag="ACHK8E_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0344541.1"
                     /translation="MANRKQHRAIAERRHIQTEINRRLYRALTIARVMYFNMLCDQGC
                     RIAPDYIASTFSYLADDLRDMQRLVNEPGNNP"
     gene            complement(5862..6113)
                     /locus_tag="ACHK8E_002278"
     CDS             complement(5862..6113)
                     /locus_tag="ACHK8E_002278"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0344542.1"
                     /translation="MFSLIQRGKRYVDAAGYPVKICFCSADKVVYRRLDNQTRSVCIA
                     EFNQNFERLDYHEYIRIKAEIEQTEHLRELRCLYKRGGK"
     gene            complement(6754..6900)
                     /locus_tag="ACHK8E_002279"
                     /pseudo
     CDS             complement(6754..6900)
                     /locus_tag="ACHK8E_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6897..7251)
                     /locus_tag="ACHK8E_002280"
                     /pseudo
     CDS             complement(6897..7251)
                     /locus_tag="ACHK8E_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7253..7597)
                     /locus_tag="ACHK8E_002281"
     CDS             complement(7253..7597)
                     /locus_tag="ACHK8E_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0344543.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTSAGIRIEGE"
     gene            complement(7584..7889)
                     /locus_tag="ACHK8E_002282"
     CDS             complement(7584..7889)
                     /locus_tag="ACHK8E_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0344544.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEWD"
     gene            complement(7886..8167)
                     /locus_tag="ACHK8E_002283"
     CDS             complement(7886..8167)
                     /locus_tag="ACHK8E_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0344545.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIAEKFGMKLRTVNNYVYFDRRVQA"
     gene            complement(8200..8916)
                     /locus_tag="ACHK8E_002284"
     CDS             complement(8200..8916)
                     /locus_tag="ACHK8E_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0344546.1"
                     /translation="MESVIDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVTETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVIVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(8900..9121)
                     /locus_tag="ACHK8E_002285"
     CDS             complement(8900..9121)
                     /locus_tag="ACHK8E_002285"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0344547.1"
                     /translation="MSKLSEEECDVLEFLLASGRNTARSVAETLQMSSATVVEILKRL
                     ETEGLVKQLNGFWSAQEERNTGGKHGKCD"
     gene            complement(9114..11210)
                     /locus_tag="ACHK8E_002286"
     CDS             complement(9114..11210)
                     /locus_tag="ACHK8E_002286"
                     /inference="COORDINATES: protein motif:HMM:NF018353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP0344548.1"
                     /translation="MAEEKNQEYAYAFPVKNEATRKRLGFTHAFYWKSDYSQARVLLR
                     ANVAMLDAGFEPEDFKKPVRVNLPVVSELPAEGVFDTEFCRLYVPGGEDGKTMVYTRA
                     AEAVADREETNSASNTNVNGEDMAEIEDNMLLPVSGQELPIRWLAQHGSEKPVTHVSR
                     DELQALHIARAEELPAITELAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGF
                     MTAFFEAYLDTDYTDRGLLTKEWCNGNRISRVENKKEAQEKVVEKSPVTGERPRRSEK
                     PTFRAINYEIAAALCPGADCHDLRPVMDSAKKLMADDDERWRAWSTALGVTEGIKSWD
                     RKTIFDMVSLAPSHIATGAAEARREWIENFLIARGVRDPDWAEPLVCGSVPGHEDNLK
                     RVREAGKRLRKIENSHTTSQSDCDLRKTGATEVDESKATSSAEIPDADKLLAASCGEY
                     VDGISDPNGQNWVPGIQTRETNAIVQDSACDTSQESEKANQNVETANHSEPDIVTEGP
                     ELQENEPEVQGTEPEAARENGCGGCGKTGGGHCPDCGAVMGDNTYSAVYGEATGDVAE
                     RGVVNVQHKVTADEVRKIMKAVKPSVHTDTEESTEEMNGELPAPQTDDNDDRSPGATS
                     EDATTNVFDDSEALLHEEDFRGGDEADVDVRALLDILRQQQVMLEEIRSTQARFTGAV
                     IAMLSAISGGEQVSHE"
     gene            complement(11216..11431)
                     /locus_tag="ACHK8E_002287"
     CDS             complement(11216..11431)
                     /locus_tag="ACHK8E_002287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344549.1"
                     /translation="MDKAQRDAAVAANRHKVSPEQVLIWCEQLKFIVDTANRNTRHNE
                     KSRALEPILAWIEQQKKQAMAEIRKRG"
     gene            complement(11433..12032)
                     /locus_tag="ACHK8E_002288"
                     /pseudo
     CDS             complement(11433..12032)
                     /locus_tag="ACHK8E_002288"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(12019..12174)
                     /locus_tag="ACHK8E_002289"
     CDS             complement(12019..12174)
                     /locus_tag="ACHK8E_002289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344550.1"
                     /translation="MKLFSLRKNKKTEKKKRTYPANNNVRLLRYYQKLQVLSCDPDKK
                     FSLDVWV"
     gene            complement(12970..13155)
                     /locus_tag="ACHK8E_002290"
     CDS             complement(12970..13155)
                     /locus_tag="ACHK8E_002290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344551.1"
                     /translation="MEKEKLKHFRKGLKDSQRVMAIAVKRLNDGRYKSAEELMRGEAA
                     SLFKLADELSDVTEQQS"
     gene            complement(13337..13729)
                     /locus_tag="ACHK8E_002291"
     CDS             complement(13337..13729)
                     /locus_tag="ACHK8E_002291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0344552.1"
                     /translation="MAVKKKKSLCIDGSSADLVFLDKGAACVGLTREALATALRRGEM
                     PGHKKRKNPEDESSRGVWLFNIKAWNELADILPDIEPQEWHHWKDYWLYNRNTGKFQK
                     VDKSDCVEIEGRRVYKPRLSRMERKKQH"
     gene            13890..14204
                     /locus_tag="ACHK8E_002292"
     CDS             13890..14204
                     /locus_tag="ACHK8E_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0344553.1"
                     /translation="MMNMSIDYSQKLRVIRKSENLTQAKFAEITGISLGTVSNYESGF
                     RPARIEIVEKVILVSRFEKYTMWLLHGKTFPQAGQIAPALSLDGSGESEVNQASAKTT
                     SK"
     gene            14304..15293
                     /locus_tag="ACHK8E_002293"
     CDS             14304..15293
                     /locus_tag="ACHK8E_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0344554.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRIRKIFTLKSKAQDYEKYVLQ
                     NMHNKPWLEKPADKRHLSELAEMWWMLDGQNQPYGETYLQRLNKVIREMGDPRSNCVT
                     RKFLLGYRANKLSAGLTPNTVNRDLCVLSAMFSVLINADEFHHANPLHGLRKLRIVPS
                     DMSFLSNEEIELLLAHLTGDNKRIAILCLSTGARWGEATKLRGENIVGNRVMFNQTKT
                     NKPRAIPISEEVLKLIKIKQSGALFDVNYTNFRRALKEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHSTIQQTMTYAHFAPDFLQDAISFNPLAESVHNLSI"
     misc_feature    15458..15583
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            15459..15506
                     /gene="pheL"
                     /locus_tag="ACHK8E_002294"
     CDS             15459..15506
                     /gene="pheL"
                     /locus_tag="ACHK8E_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP0344555.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            15605..16765
                     /gene="pheA"
                     /locus_tag="ACHK8E_002295"
     CDS             15605..16765
                     /gene="pheA"
                     /locus_tag="ACHK8E_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP0344556.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(16808..17929)
                     /gene="tyrA"
                     /locus_tag="ACHK8E_002296"
     CDS             complement(16808..17929)
                     /gene="tyrA"
                     /locus_tag="ACHK8E_002296"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP0344557.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(17940..19010)
                     /gene="aroF"
                     /locus_tag="ACHK8E_002297"
     CDS             complement(17940..19010)
                     /gene="aroF"
                     /locus_tag="ACHK8E_002297"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP0344558.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            19220..19585
                     /gene="yfiL"
                     /locus_tag="ACHK8E_002298"
     CDS             19220..19585
                     /gene="yfiL"
                     /locus_tag="ACHK8E_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP0344559.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            19735..20253
                     /gene="yfiR"
                     /locus_tag="ACHK8E_002299"
     CDS             19735..20253
                     /gene="yfiR"
                     /locus_tag="ACHK8E_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP0344560.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            20243..21469
                     /gene="dgcN"
                     /locus_tag="ACHK8E_002300"
     CDS             20243..21469
                     /gene="dgcN"
                     /locus_tag="ACHK8E_002300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP0344561.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            21485..21967
                     /gene="yfiB"
                     /locus_tag="ACHK8E_002301"
     CDS             21485..21967
                     /gene="yfiB"
                     /locus_tag="ACHK8E_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0344562.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(22044..22391)
                     /gene="rplS"
                     /locus_tag="ACHK8E_002302"
     CDS             complement(22044..22391)
                     /gene="rplS"
                     /locus_tag="ACHK8E_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP0344563.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(22433..23200)
                     /gene="trmD"
                     /locus_tag="ACHK8E_002303"
     CDS             complement(22433..23200)
                     /gene="trmD"
                     /locus_tag="ACHK8E_002303"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP0344564.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(23231..23779)
                     /gene="rimM"
                     /locus_tag="ACHK8E_002304"
     CDS             complement(23231..23779)
                     /gene="rimM"
                     /locus_tag="ACHK8E_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP0344565.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(23798..24046)
                     /gene="rpsP"
                     /locus_tag="ACHK8E_002305"
     CDS             complement(23798..24046)
                     /gene="rpsP"
                     /locus_tag="ACHK8E_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP0344566.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(24183..25544)
                     /gene="ffh"
                     /locus_tag="ACHK8E_002306"
     CDS             complement(24183..25544)
                     /gene="ffh"
                     /locus_tag="ACHK8E_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP0344567.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            25711..26502
                     /gene="ypjD"
                     /locus_tag="ACHK8E_002307"
     CDS             25711..26502
                     /gene="ypjD"
                     /locus_tag="ACHK8E_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP0344568.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            26523..27809
                     /gene="yfjD"
                     /locus_tag="ACHK8E_002308"
     CDS             26523..27809
                     /gene="yfjD"
                     /locus_tag="ACHK8E_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP0344569.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(27863..28456)
                     /gene="grpE"
                     /locus_tag="ACHK8E_002309"
     CDS             complement(27863..28456)
                     /gene="grpE"
                     /locus_tag="ACHK8E_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP0344570.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            28579..29457
                     /gene="nadK"
                     /locus_tag="ACHK8E_002310"
     CDS             28579..29457
                     /gene="nadK"
                     /locus_tag="ACHK8E_002310"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP0344571.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            29543..31204
                     /gene="recN"
                     /locus_tag="ACHK8E_002311"
     CDS             29543..31204
                     /gene="recN"
                     /locus_tag="ACHK8E_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP0344572.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            31353..31694
                     /gene="bamE"
                     /locus_tag="ACHK8E_002312"
     CDS             31353..31694
                     /gene="bamE"
                     /locus_tag="ACHK8E_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0344573.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(31756..32046)
                     /gene="yfjF"
                     /locus_tag="ACHK8E_002313"
     CDS             complement(31756..32046)
                     /gene="yfjF"
                     /locus_tag="ACHK8E_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP0344574.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(32036..32473)
                     /gene="ratA"
                     /locus_tag="ACHK8E_002314"
     CDS             complement(32036..32473)
                     /gene="ratA"
                     /locus_tag="ACHK8E_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP0344575.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            32644..33126
                     /gene="smpB"
                     /locus_tag="ACHK8E_002315"
     CDS             32644..33126
                     /gene="smpB"
                     /locus_tag="ACHK8E_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP0344576.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            33341..33703
                     /gene="ssrA"
                     /locus_tag="ACHK8E_002316"
     tmRNA           33341..33703
                     /gene="ssrA"
                     /locus_tag="ACHK8E_002316"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(33864..38438)
                     /gene="ypjA"
                     /locus_tag="ACHK8E_002317"
     CDS             complement(33864..38438)
                     /gene="ypjA"
                     /locus_tag="ACHK8E_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP0344577.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGYLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQVLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHTHIFFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     SLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(38503..38622)
                     /locus_tag="ACHK8E_002318"
     CDS             complement(38503..38622)
                     /locus_tag="ACHK8E_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0344578.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(38777..39043)
                     /locus_tag="ACHK8E_002319"
     CDS             complement(38777..39043)
                     /locus_tag="ACHK8E_002319"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0344579.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
     gene            complement(39374..40828)
                     /locus_tag="ACHK8E_002320"
                     /pseudo
     CDS             complement(39374..40828)
                     /locus_tag="ACHK8E_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
BASE COUNT        10221 a        11029 c         8918 g        10679 t
ORIGIN      
        1 gtcgaaaagt ctatcgtaaa ttcccgggaa taggataccg aagaaaagcc cagtaacagg
       61 cacagtaccc attttaacaa tatacacttc atatacaggt attccttttg gctgaagtaa
      121 tcagcaccag acccggcgca gatataaaaa aggcccgcaa aagcgagcca gaaaaaataa
      181 gtatggcgcg ttgtactgga ttcgaaccag tgacctggca attatcagtc gcttgctctc
      241 accacacgaa agcaataacc ccaataactc caaaatactt gaccgtaaac atattggggt
      301 tataataacc ccaataagaa aacacagaga ctcggaggat ggacagcaga aatgcaatag
      361 cgatgatcga agctgacggg tggtacctgg tgcgcgtaaa aggcagtcat caccagttca
      421 aacacccgac gaaaaaaggg ctggtaacag tgaaacatcc acagaaagat atcccgttac
      481 caacattaaa aagcatcaaa aaacaagcgg ggctttaagc cccaggaggt ttgtatgtta
      541 tatcccgttg ctattgataa aagcgattca tctttcggcg ttcgcgtccc cgacattccc
      601 ggctgcttct ctggtggcaa caactatcag gatgcaatcg aaagcgcaca tgaagcgata
      661 gaagcacata ttgaattact ggttgaaaac ggcgaagccg taccaaaagc cacttgtata
      721 gaaaactggc tggaagatcc agattattca gatgccgtgt gggcactggt cgatgtggat
      781 atcacacgcc tgatgggcaa agcggaaaaa atcaacgtca cactcccctc tctgctaatc
      841 cgccgcatcg atcagtttgt tgcggcgcac cccgaatacg gcagtcgctc cggttttctg
      901 tcccgcgtgg ctgcagataa aatcacagga tgaatgcaga aaaacccgct ccatggcggg
      961 tttttattct acttacaact ctggcagact atcaaatttg actaaaattt acctcaaaat
     1021 attcacttta gcaaatttat attttttctg gtgatttttt attcccataa tggttatcag
     1081 tgcctcatga tccagttcac aacacacttc atgcataaaa caccagtacc ccttgtaatg
     1141 ctgactccat gtgtttggtt ttatatctat ctgctgcgcc gcacatgaag gcgcaatcag
     1201 aactgcacct gccacaaccc caccagcaat tacggcagca ttcaacgcta ctatccttgt
     1261 cagcctgata agatttccga tcatcctttc cgtaacacgt tttccccgca accgctcacg
     1321 cacccgcatc cagatgaact cactgaccag aagatgatgt attcgttttt tctgcaaact
     1381 gtaactgaat aacacccacg cccgctgata atcctccagg caataaatct ttctcctcaa
     1441 tttagctgtt ctgaatgcct ccggggtgat catctctgac tgcgcagagt agcggggggg
     1501 ttcagccgca aataccggct cgctgtcccg gcaataccag cgaccatcaa tattcagtgt
     1561 tctttttctc tgcctcccgt aacgttttgt gtctgaccgg gcaaaatcac caaatggctc
     1621 cagttgccct ttctgacgta tatccggtgg attcagcgca aaaataacct gttctcttgc
     1681 ccataccatc tgctgtggtg tcatattacc ctccagccaa agcgccaata cccgcagctc
     1741 tgtccagtgt ccgtaacacc cacagtagct ggctgccgtt cctctcctcc catccctttc
     1801 tgtcgttgtg caatgcctga tgacattccc ggcaaagcgg tatcacaaac aggtcatggg
     1861 cttttatccc tgcaccgcca aatccatgcc ctgtgatgtg atgcggatca tctgccacct
     1921 ttccgcaccc acagcaccgc tgtcgtttca cccatgctgt ataatccggc agaaccagac
     1981 gctgcatcct cggccttctc atatagccca tcaccgggtc actgtcagtt gccagtttca
     2041 gtactgctct ttcctgctca tccgtttttt cccgacgtac tgcatgagca accagagcat
     2101 tgtgcgtact tacgggcttc gccctctctg ccagtttctt caccactgcg tcagcagata
     2161 tctgccaggg gttaatatcc gcctccctca tcgttcccct cagttgttcc tcctccggca
     2221 gtctcctgat acgccttacg atctcaaccg gaacagcatc tgccacatca ttcagcacag
     2281 cccagcaaaa caactcagga atggatacct gatgatcttc ccccagtccc agattcatcc
     2341 tgataaaatc catcagccat tccgcacgat ttctggcaat cacatctgcc agctcccggt
     2401 atcccttcat ataatgtcga ttatcacagg catggcataa ccggatagcg cctccctgca
     2461 ttgtccgcgc tgtcatcatg ttctccacat gatcgccatt ctcacactga catcccgcaa
     2521 tcctgctcac ccaacgagcc agggcatcac tgccaccagc acgctttatc accctgtcat
     2581 ctgcaaaaaa atccttcagt tcctcttctc ccgccagtcc ctgtcctgat gacggaattt
     2641 ctcctgatgg catccctgcc agcacagcag ggacatccct tatcattact ctctgcccgt
     2701 ccctgaaccg tgctgtcagg ttgcctgttt ttataagtgt tatcccggca ttacgctgcg
     2761 ggaataccgt caggatcgcc tgcatatcac acctcttact gttcagtacc cacaatatcc
     2821 tgaacaattt tttcaagtgc gcttccatac ttttccagct ctgccgcctt atcgatcatc
     2881 tgatcaacca ccagttgcag ttcatgtttc gcacggttca gacgctgcac cgccggaagc
     2941 aggttttttc tgacagcgtc tttcgtcatc cccgcctgcg ccatagcctc agatctcctg
     3001 atgcactcca taccaattgc gtgcattttt tccggcgtca ttctttcaag ctccagccct
     3061 ctcatgatcc acctcatctt tcactcaact gtcgccatat gacgacactg tatataaaaa
     3121 catactcagg taaaacaggt atttttcttc tccggacacc ccactttctc cactgcctta
     3181 accggaaaat ttagtgctca ttttttcctt ttttctcctc ccagaaattc agctccagct
     3241 gaacagggat ccttatacaa agatccggag gatgatcggc aggtcgtttt ctgcccgaaa
     3301 tgcagttatt gacacaagtc cagggcgtta aagcaggcca tctgttaaca ccgagagagc
     3361 ctgttgtaac gttccggctg attctccagt tacgtgaacg cgtcactacc ggtggtgtat
     3421 cacaccccgg catataaaca cctttgatcg cactggcgtg acgtccacac tcatccggct
     3481 cttccgaacg ctgataccat gtgcgaagaa ccagatcact tcgggcaaca aacgggccac
     3541 cctgcagacg ggtataggtt ttccagtcac cttcactcgc agcccggtgc aattctccca
     3601 gcgccggact tatcctgtca gcatgcacct gattgttaaa acgacgcagt tcccgccaca
     3661 cactcaccgg agcactgcca ataaactgaa actgctttat tccccacaac gatgcccatg
     3721 ccgaagcatg gcgggctgtc accaccagcg tatggcccgt ttcatggttc attccgccgg
     3781 acgcacaccc gtcaatattc attccgatat gtttggcgat ataggcagtg gcacttccca
     3841 gatcatcaag aaccggtttt atctccagat gggattttct tcctgctttg cggatggtca
     3901 ctttttccgc gtcatcatcg cggttagcgt aatactcaag aatttcctgt aaggcgacgg
     3961 cgtactcctg atgcacataa agaacaccat gccagtgcgg cgtaccgtca tgatgcggtt
     4021 cggcactcct cagaccaaaa accccaatcc cgcggcgcct cagcgttgca ctgatctgac
     4081 gccatagccc ggaaaaataa ttctgagtgt ctttagggct tgcgccgtta taacgacggt
     4141 tgcggtatcc cgtctccagc caggcgtgat atttcgatgg tgccgtcatc gtgaaaaaca
     4201 gcccgacata cccctgctcc tgcgccacac cttcaaagcg tgccaggtct tccagcccct
     4261 ttacacgcgt catcaactcc gaccgacgtt tctccgcacc tgaaatgctg ctgtcaacca
     4321 aatcgagaag tgaaaatttc tccccggttt cttcatcctc aacgtctgtt tctgccatca
     4381 gcctgcgacc atgcttacgt cccgccagcc aacgctgcac ccactcataa ctacagtgcg
     4441 gtgatgagtg ccgacgaacg ctccccatcg ctataagcag atgttcacgc cagcgtcgag
     4501 aaaatacccg aagttttccc tgccaccagc gaacactcag catccgactg atggcaacag
     4561 tcagttgatc cagcgtcatc tttctgccaa taaaacgttt ccagtccggt ggctccatac
     4621 ggaaatgttc aacaagaccc gcaaccatgg cataaatggc acctgccgcc acatccgcat
     4681 cttcatccgc aagaagagca ctcagatgct gcatttcagc aataagaaac gcggacagat
     4741 cctgcgccag taaatgtgca tccccgtcgt ctccatccac cagggaattg aatcgccatg
     4801 tcatggaatg aaccgcgccg tatgcctgat cagacgcaag gccaccaaac agccagcccg
     4861 gtaattcatc cggattaatg gcataacgtt tattcacacc agtaatacac ggcaaatcct
     4921 tctgaaccat caacgcaaac ttttcccggg caccggcgaa cccctttgtc tgccacgcgc
     4981 tgctgatccg gcgtcccagc tttacagaca caaaacgtgg cagtccggaa aaagcgctgt
     5041 tcacctcctc agtgaatatc aactcttctt ccggctcagg catgcgcgga cctcccgctg
     5101 aatacacggt catatgtcct cccgcataac gggtaaccag acaccccgcc agatggcgga
     5161 gtgccgtttc aggatttcag gaaaaagagc tttacgggtt attccccggt tcgttaacca
     5221 ggcgctgcat atcccgcaga tcgtccgcca ggtaactgaa cgtggaggca atgtaatcag
     5281 gtgcaatccg gcatccctga tcacaaagca tattgaagta cataactctg gcgatggtga
     5341 gagcccggta caatctgcgg ttgatttcgg tctggatgtg acgacgctcc gcgatagcgc
     5401 ggtgctgttt acggtttgcc atggtgtggc ctcgtacgta gtgagttgtg ataacccaca
     5461 gccgcgagtt gagaatctcg gggtggcagg acgtatgggg ttctcaacac cggctacgca
     5521 cgactccggc ccgaccaaag tcggccccac acgccccacc atgattcttg cgagaaaaag
     5581 atgtggcgat acggtacgca caaaaaaacc gctggcgcgg ttgtgcgcgt gcatagtcag
     5641 cgggttgaga atcccggccc ccgttttatg aggtgcaacg gaaatgtaac ccgaccaggt
     5701 gaacactggc aagcggtttt tttgtatcgt cactggcttc acttctgcta tttctcttat
     5761 cccgttttaa ggttgcagga atcccgccgc atgagcggtg tatttctcat tcttcttatt
     5821 tccggttaat tcaggcgaag gttaaatttc tgtatccgta tttatttccc acctcttttg
     5881 tataaacatc tcaattcccg cagatgttca gtctgctcaa tctctgcttt aatccgtata
     5941 tattcgtgat aatccagccg ttcaaaattc tgattaaatt ctgcgataca gacagaacgt
     6001 gtctgattat ccaggcggcg atacaccact ttatcagcag aacaaaaaca aattttcacc
     6061 ggataaccag cggcatcaac ataccgctta ccacgttgga tcagagagaa catgtattta
     6121 tcccccagcg gcaaattgag tacacaacca gcacaatcgc caacgcaata ccaacatttg
     6181 agaacacttc aagcctggtc atcgtaaaat actctctgtg tttatcagcc cattctgttt
     6241 cagatactcc atcgccgtat caggtagctt gcttgattta ttgacgcttt ttaatgaact
     6301 ggctaatcgc ttaaccaaca tagttaattc actcacctga tcgcctaatg ctggattatt
     6361 gtcgcttttt cccggcaata tgtgctctcg actggatttc cagaatatcc acgccaaatc
     6421 cacatttgga tcccggtaat tcatcccttc gttagcacga agcaataatg tttcatggtt
     6481 tttatcatct gcatggaacc tgataagtgt ttcgaactgt tcgcgaccgt catcagggat
     6541 tacctgagag ttacccgtta gcgcattctg ctccagtgat gcttttacaa accaggctgc
     6601 ctggactata accccatgga tccagcgcaa atcagcatcg cgctccttct tcttcatctt
     6661 ttcgccactt aaagccttgc ttatgtggct gcgtaccagg tcttcatata actccttcgc
     6721 atcctcaatg gtgaaaccac caggcaggcg agccggagtt accggagagt tgccagcctt
     6781 acgcatggcc atttccacga tttcaagcat atctcctggt ggaattttac aaagttgccc
     6841 aatacatttc tgctgcctgg catattcgag aatgtgctcc agtttggttc gatcaatcat
     6901 gatttatctc cattaagcat tgcagcacgg caatcgttcc atccttctgc ataatcacta
     6961 tgcgcaagag gccaacctct tatgtattca cgcggcaact tatcaggcac tggcggggcg
     7021 gcgtatagct ggtgcgttcc atcaggcagc gagtgaccaa catactcacc gaacccgtca
     7081 acacacatgc cccccatctt caataatgca tgacgccaca ggctctgctt ccagcgatgc
     7141 cagtgcgata agcgccagct cacgaatttc accgccgtct atatcgtcaa tgtcatcgcg
     7201 gccagaaatg ttagccagcc attgcagtcg ctctttggta atagtggtca tctcactctc
     7261 cttctatgcg aataccagca gaagtcagtg cggcgttgac ctcatcagcg tagtagtacg
     7321 ttaatccact tgatgattta gccagcttga atggttctgg taacttcact gtgcgggact
     7381 cctgctcgtc gatacgctgt tttgcttcct ccagttcatc cagcaaatca gcgataatat
     7441 ccacttcacg atgacggatt tcacgcttaa acgcagccaa agctgcatca caatcctgct
     7501 cagcgtttgg actgtctggt ttctcctgat accacgccag catcgactga tagttttgtg
     7561 ccgcctcacg cagcgcctga taattaatcc cactcactgg ttgcctcctg gaaaataact
     7621 gcatgcccca gtttctccgc cagtgccagt tctgccttag cgcctgccga ccgctgccag
     7681 cctttcagca tgtaaaccgc atccacacaa cgaatcatcg ccatgcaaat atccatgtaa
     7741 tgtggctgag tcagcccgtc cggaagtact gcgggattta atacggtatg cccttcctgt
     7801 ttcagtgctt cttccgcctt gtgaaacgcc tcacggttga aattttcata tcccgtcatc
     7861 ggaccggcga tataaattct caccctcacg cctgaaccct cctgtcgaaa taaacgtagt
     7921 tattcactgt gcgcaacttc attccgaact tttcggcaat ttcccttctt tgcatgcctc
     7981 tctgatgcag ttgccgcgcc agctcaatat cacgctgaga atattttgcc gacgggtgaa
     8041 aatcaccacg taacatcatg ctgatgccca gctcccgtgc cttcgttctc actgccgctt
     8101 cagttcgtcc gataagtgcg ccaatgcttt tcaccttcat agttcccgca cactgacgga
     8161 gtatcaggat ttcagcccgg caccacgtct tccagccact caccgctgct gttctctggt
     8221 ggcggtaata tcccggagaa tatcccggca cttgttcagc tcccgcagcg cggcgcagac
     8281 acgctcccac ttctgaacct gaccttttgc ccggcgcagc tcgcggttgg ccacatgcag
     8341 cgatggtaaa atcagaccat ccggatgctt tctggtgaac gacggctgtg actgcactat
     8401 gaccgccaca ctttcagttt ttatttcttc ctgtgtttcc gcttcccgga ctggtaacgc
     8461 aacacctgct ggctgaggaa aggctttacc atcggtttcc gttaccgatg cagcttccgg
     8521 ctctgccggt aaatcagcac ccggtatgca gtaacgaaat ttaccgccct gattcacgcg
     8581 aatcagacgc cctttgctga ttgccatggc cagcgatgaa ttcgcccggc gggaggtaat
     8641 cccgaacatc agtgccagct catccgccgt ttgtgggcca tgttgttcaa tcgcctcagt
     8701 cagcatttgc gctgtcactt tcggtaccgg tgacactggt tcactttcac cagcctgagt
     8761 cagccaccac atcgaaccct tgttatccgc ttcaccacgg cgcttcagtt tccacagttc
     8821 gttgacagca tcttcgcggc tgattccaag acgggccgcc actacctgtg aagaggctct
     8881 tttcagtgct ttcagtgcat caatcacact ttccatgttt acctcctgta tttctctctt
     8941 cctgcgcaga ccagaaacca ttcagttgct ttaccagtcc ttctgtttcc agccgtttca
     9001 atatttcaac caccgtcgcc gatgacatct gcaacgtctc agccacactt cgtgcggtat
     9061 tgcgtcctga tgccagcagg aattccagta catcgcattc ttcttcactt aacttactca
     9121 tggctgacct gctcccctcc actgatagct gaaagcatgg caataaccgc ccctgtaaaa
     9181 cgagcctgcg ttgagcggat ttcttccagc attacctgct gctgacgcaa aatatccagc
     9241 aatgctctga catccacatc cgcctcatca ccacccctga aatcttcctc atgaagaagc
     9301 gcttcactgt catcaaaaac atttgtggtc gcgtcttctg atgtcgcccc tggagaacgg
     9361 tcatcattat catctgtctg cggtgctggc agctcaccgt tcatctcttc cgttgactct
     9421 tcagtgtcag tatgaacaga gggtttaacc gctttcatta ttttcctgac ctcgtcagcg
     9481 gtaactttgt gctgaacatt cacaacgccc cgttccgcca catccccggt agcctctcca
     9541 taaacggcac tgtatgtgtt atcccccata actgcaccgc aatccgggca atgacctcca
     9601 ccggttttac cacatccacc acaaccattt tcccgtgctg cctccggttc cgtgccttgc
     9661 acttctggtt cattttcctg caattccggc ccttccgtca caatatctgg ttcggaatgg
     9721 ttggcagtct ccacattctg gtttgctttt tccgactcct ggctcgtatc acaggcagag
     9781 tcctgaacaa tggcgtttgt ttcccgcgtc tgtattcccg gaacccaatt ctgaccatta
     9841 gggtcactga tcccatcaac atattcacca caggaagcag ccagtaattt atcggcatcc
     9901 ggtatttcag cagatgacgt cgctttgctt tcgtccactt cagtcgctcc cgtcttccgc
     9961 aagtcgcagt cgctctgcga cgtggtgtga ctgttctcaa tcttcctcag acgttttcct
    10021 gcctcgcgaa ctcttttcag attatcttca tgcccaggca cagaaccaca tacaagcggt
    10081 tctgcccagt ctggatcacg aacaccgcgc gctataagaa agttttcaat ccactccctc
    10141 cgcgcctctg ctgcccctgt tgcgatatgt gacggcgcaa gactgaccat gtcaaaaatg
    10201 gtctttctgt cccagctttt aatcccttcc gtaacaccaa gggctgtcga ccatgcacgc
    10261 catctttcat catcgtcagc catcagtttc ttcgcggaat ccatcaccgg acgtaaatca
    10321 tggcagtccg caccaggaca taaagcagca gcgatttcat aattaatcgc tctgaaggtt
    10381 ggtttttcac tgcggcgcgg tctttcccct gtgactggcg atttctccac aactttctcc
    10441 tgcgcttcct ttttattttc aacgcgggat atgcggtttc cgttacacca ctcttttgtc
    10501 agcaggcctc gatcggtata gtcagtgtcc aggtatgctt cgaaaaaagc agtcatgaac
    10561 cccagactgg aattaacggg attagggaaa actttgtcag tgtcgcgcac cagtttgtga
    10621 aggtcgcgaa tctccagcgg gtcgagcagc tttgtgttgt gggaaatggc cagttcagta
    10681 atcgctggca gttcttcagc ccgggcgata tgcaatgcct gaagttcatc ccgtgatacg
    10741 tgcgttaccg gtttttcgct gccgtgttgc gccagccagc gaatgggcag ttcctgaccg
    10801 gaaacaggaa ggagcatatt atcctcaatc tcagccatat cttcgccgtt gacgttagtg
    10861 ttactggctg agttggtttc ttccctgtca gccactgctt ctgcggcgcg ggtatacacc
    10921 atcgttttac cgtcttcacc acccggcaca tacaggcgac aaaattccgt atcaaaaaca
    10981 ccttccgccg gaagttcgct cactacagga agatttaccc gcactggctt tttaaagtcc
    11041 tccggctcaa accccgcatc cagcatggca acatttgccc gaagcagaac tctcgcctgc
    11101 gaataatcac ttttccagta aaaagcatga gtaaacccca ggcgctttct ggttgcttcg
    11161 ttttttaccg gaaacgcata tgcatattcc tgattttttt cttctgccat ttttatcacc
    11221 cccgttttct gatttccgcc attgcctgtt tcttctgctg ctcaatccac gcaaggatcg
    11281 gctcaagcgc cctgctcttt tcgttgtgcc gggtattacg gttggctgtg tctacaatga
    11341 acttcagttg ttcacaccag atcagcacct gttccggcga gaccttatga cgattcgcag
    11401 ctacggctgc atcacgttgt gctttatcca catcacacct ccgtcacacc gcgaagtgct
    11461 gcaaataccg tggtcacatt tcttatctgc cccataacat cattaagggc atgatgggtg
    11521 acatcctttc tggtgtaagg caacgggata ccaagatgcg tggcaagccc ttcaaccgtt
    11581 ctcacgtctc tctcattcca gaacggccac agcatttctg cactggcatt taccccaata
    11641 ctcctgtcgc acatgtcacg aatcagctaa cagtcaaaca ctggtgactt cgcccatacc
    11701 cgcacccgac ggatatcagc atgctgctca ataaagttaa tcagcgcata tgtgccatca
    11761 cgctcagaaa cacattcatc tttccttact gcttccagtc gcgtttctgt cgattgctcc
    11821 agccagaaac agaatgtctc cggatcaatc actgcgccat tgcgtgtcgc acttacccag
    11881 tccagtcgga gataaatgga atctccttta tctccggtat caggattaaa aaatgctgcc
    11941 gcaaaggaaa taatccccga atgatgggat gttcctgtgg tttcagtatc aatcatcaga
    12001 tcgttcatag ttcccccatt acacccacac atccaggctg aactttttat ccggatcaca
    12061 acttaatacc tgtaatttct ggtaataacg caacagacgg acgttattat ttgccggata
    12121 agtgcgtttc tttttttccg tttttttatt tttacgtaat gaaaacaact tcatgtttcc
    12181 acctcgtcag atattgaaga tgttagtatc ccgccgtgtg agcggtgtat ttaatactta
    12241 ctgacaattt tgattaataa caaagaaact atcgtcgttt ccttttcccc ataacctggt
    12301 caaacaagcc atgagaaatt aactcattat ctatagcctc aatatcatcg aagatgtaag
    12361 acaataagta cggaaaacaa agatcgactg agcaggagtt atcagcgaga cgctgaatgt
    12421 aataatgacg cgtaaccatc tgcacacgat gaatccggcg acgacagtca cagctgagtt
    12481 gggggatctc gaattttggg cgcttttagg ttgggattgt agccattgcg gcagcctccg
    12541 ttgacctgac gtaccgctat cgcaggaggt gagatgctca ctggcgatag cccagacggg
    12601 gatctcacta ccggcgtcaa cggaaaccgg cccgacagaa gtcggccccg cctgagctac
    12661 catagacatg gaggtgggtg ttatccggcc ccttccataa gaaaggtttg cgacggcacc
    12721 agttggataa caccgcacca caatatggcg catcatggcg tataaggtta cgacaacaaa
    12781 aaagacgctt gcggcgtctg atgtcgccgt tgacaaatcc ggggtgagat tcccgactgc
    12841 cagaactgtg gcagcagtag aaaaatacca ccatgccgac actacattca agctcttttt
    12901 tgtctgcctg ctcattttgc acgtaacctc acgctttctt aatcacacga gaccgtaact
    12961 ttctctggtt catgattgtt gttctgttac atcgcttaac tcatctgcca gtttaaacag
    13021 cgatgcagct tctccgcgca taagttcttc tgctgattta taacggccat catttaatcg
    13081 cttcaccgca atagccatta cacgctgaga atcttttaat cctttacgga aatgtttcag
    13141 tttttctttt tccatatttt ccgccttagt tattgatttg acctcatccc caaaaaaacg
    13201 gaattccctt tccgtgccca atatcatcga caaaaaattc acctcgacca atagctcacc
    13261 aagtctgtac agaaagttat ctgctattta ttactgcatt aattcaatgt gatttcgtgt
    13321 tgcagcctat agacagtcag tgctgtttct ttctttccat tctcgacaaa cgaggcttat
    13381 aaacgcgcct gccttctatc tcaacacaat cacttttatc aactttctga aactttccgg
    13441 tattacgatt gtacaaccag taatccttcc agtgatgcca ctcttgtggt tcaatatccg
    13501 ggagaatatc agccagctcg ttccaggctt taatattaaa aagccacact cccctcgatg
    13561 actcatcttc cggattttta cgcttcttgt gccccggcat ttctccacga cgtaaagcag
    13621 ttgccagcgc ctctcttgtc agacctacgc acgctgcccc tttatcaaga aaaaccagat
    13681 ctgcgctcga tccatcaata cacagagatt ttttcttttt caccgccatt acctctcttc
    13741 caccagctct cctgaatact tcccctctgc aatagtgttc tgcattttgt tattctccag
    13801 aattggtgcg tttgctggct gttgtcggct attgtcagcc aatgcaaaca catcacatga
    13861 tgagatttca aaaagaggat actcaccgta tgatgaatat gtcaatagat tactctcaaa
    13921 aattgagagt aattcgaaaa tcagagaacc ttacccaagc aaaatttgca gaaatcaccg
    13981 gtatatcgct tggtaccgta tcaaactatg agtcaggctt ccggcctgcc aggattgaga
    14041 ttgttgaaaa ggtcattctt gtttcccggt tcgagaaata cacaatgtgg ctcctgcacg
    14101 gaaaaacctt ccctcaggcc ggacagatag ccccggctct ctctcttgat ggctctggag
    14161 agtcggaggt caatcaggct tcagccaaaa ccacctcaaa ataacgccaa taacaccaca
    14221 gtgctggctt ggccttcatg ttgaatacat gaccaggcat aagagagttt tgaaacaaca
    14281 tacatccgcc ggaggtgcta atcatgtcga ttaagcagct caaagacgga cgttatcagg
    14341 ttgatgtcag acctcagggc gcggaaggga agcggatacg gaaaattttc accctgaagt
    14401 cgaaagcgca ggattatgaa aaatatgttc ttcagaacat gcataacaaa ccttggctgg
    14461 aaaagcctgc ggacaaacgc cacctgtccg aacttgccga aatgtggtgg atgctcgacg
    14521 ggcaaaatca gccttatggt gaaacatatc tgcaaagact gaataaagtg atcagagaaa
    14581 tgggggatcc ccgatccaac tgcgtaaccc gaaaatttct tctaggttac cgcgcaaata
    14641 aactcagtgc gggactgaca cctaacacgg taaacagaga tctctgcgta ctttcagcta
    14701 tgttttctgt cctgattaat gccgatgagt tccaccatgc aaacccgcta catggactca
    14761 ggaaactgag gattgttccg tcagatatgt cgtttttatc aaacgaagag atagaacttt
    14821 tgcttgctca tcttactgga gacaacaaac gaatagcaat tctatgtctt tcgacgggtg
    14881 ccagatgggg agaagcaact aaactacgag gagaaaatat agtggggaac agggttatgt
    14941 ttaatcagac aaaaaccaac aaaccccgcg caatacctat atctgaagag gtgctcaagc
    15001 tcatcaagat caaacaatcc ggtgcgctgt ttgatgttaa ttacacaaac ttccgacgag
    15061 cattaaaaga agttaagccc gatttaccta agggccaggc aacccatgta atgcggcata
    15121 catttgccac acactttatg atgaacggag ggaatatagt aacactacaa cgaatactag
    15181 gacactcaac catccagcaa acaatgactt acgcacattt tgctccggac tttttacagg
    15241 atgcaatcag ctttaacccg ttagctgaaa gcgtccataa tctgtccatc tagttgtaat
    15301 atgttgtcgc ttcttgctgt tatgttttta cctaactcat tgataaaaat aaaaacataa
    15361 agaggcgacc aaacgcttaa agcaccttcg ggcgcgtttt ttgttgacag cgtgaaaaca
    15421 gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct
    15481 tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg
    15541 aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa
    15601 cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga
    15661 aaaattatta gcattactgg cagagcgacg cgaactggcc gtcgaggtgg gaaaagccaa
    15721 actgctctcg catcgcccgg tacgtgatat tgatcgtgaa cgcgatttgc tggaaagatt
    15781 aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct
    15841 catcattgaa gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat
    15901 taatccgcac tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc
    15961 ggcgcgccag tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt
    16021 tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga
    16081 aaataccagc tccggtgcca taaatgacgt ttacgatctg ctgcaacata ccagtttgtc
    16141 gattgttggc gagatgacgt taactatcga ccattgtttg ttggtctccg gcactactga
    16201 tttatccacc atcaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt
    16261 ccttaatcgt tatccgcaat ggaagattga atataccgaa agtacgtctg cggcaatgga
    16321 aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac
    16381 tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg
    16441 atttgtggtg ttggcacgta aagccattaa cgtgtctgac caggttccag cgaaaacgac
    16501 gttgttaatg gcgaccgggc aacaagctgg tgcgctggtt gaagcgttgc tggtactgcg
    16561 caaccacaat ctgattatga cccgtctgga gtcacgcccg attcacggta atccatggga
    16621 agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt
    16681 gaaagagtta ggggacatca cccgttcgat gaaggtattg ggctgttacc caagtgagaa
    16741 cgtagtgcct gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat cacccggcac
    16801 tggattatta gtgacggttg tcattcgcct gacgcaataa cacgcggctt tcactctgaa
    16861 aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct
    16921 gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga
    16981 ttaacgccag attacgctct gacgacataa taatgtcggc atacagttgc ggatcctggg
    17041 caaacagtcg cccaaccatc gccagctcaa ggcggtaaat cggcgaagaa agcgccagaa
    17101 gttgctcaag ctgaacattt tcttctgcca ggtgcagccc ataagcaaaa gtagcaaagt
    17161 ggcgcagtgc ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca
    17221 gccgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc
    17281 catcacacca gaccacaact tgttttgcca ggctaccgct gtccgggccg aacatcgggt
    17341 gtaaccccag caccggacca tcatgcgccg ccagcatggc ctgtaatggt ccatttttca
    17401 ccgatgccag atcgaccaga atacaatctt tcggtaaagg cggtaatttg ccaataactt
    17461 gttcagtaac gtggatgggc acactaacaa tcaccattcc ggcatcggca actatctcag
    17521 ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg ataacccgat agtgtcagca
    17581 tcttctcgaa caggcgtccc atctgaccgc caccgccgac gataaccacc gggcgcagtg
    17641 acggacaaag cgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc
    17701 gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac
    17761 gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt
    17821 tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt
    17881 tatcgacttc atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct
    17941 taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca
    18001 tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg
    18061 cgcggttgct cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata
    18121 attgagcgat tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg
    18181 cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc
    18241 gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta
    18301 ccaccgcgca ggatcacatg cccgtccgga ttcccctgag tttgtagcaa cgccacctgc
    18361 cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcacgcat agcgttaata
    18421 gccgttgcaa ggctgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag
    18481 gccatttcac ggtgtgtttg cgattccgtt gtacgagcac caattgctga ccagctaaac
    18541 agatcgccca ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc
    18601 atattcacca gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa
    18661 gagccatcca tatggggatc gttaatcaac cctttccaac cgacagtggt acggggtttt
    18721 tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta
    18781 aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact
    18841 accaacagac gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca
    18901 atctgggctt cctgttgcag gcttaatgga aaagcggcct tcagttgttc cggagtcatt
    18961 aaaacttgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc
    19021 ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata
    19081 aagtaaagtt ttcaatccat atttcgtaca tttttcttta cacaagcaat ttattcgcgc
    19141 ttttaaccct tgtttatgta taaataaatt tacaccacct ttgaaaacaa tccgctatgc
    19201 tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc cccttgttgg ctttactggt
    19261 tagcggatgt cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta
    19321 tgatgttggt atggaagatg cgatatcggg caacgccata aaagatgacg atgcatttag
    19381 cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac
    19441 ttgccagacc gattttactt atgcccgagg actttccggt aaaagctttc ctgccagctg
    19501 caacaatgtt gaaaatgcca gccaactgca tgaagtatgg caaaaagggg ctgatgaaaa
    19561 tgccagcaca atacgattaa attaattaaa cccccacaaa taaattaaac atgagattct
    19621 actcattgtg ttttatttct cacattgatg acgaccgcat gaaagaatga tattttaatg
    19681 agcattctga aaaaatatcg atgcatttcg agcgaagatg gtgaggatcc ctgaatgcgt
    19741 ttttctcacc gactgttctt actgctgatt ctcctgctga cgggagcacc aatactcgct
    19801 caggaacctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac
    19861 acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc ttcacatttt
    19921 agtaacgccc tacaagagaa tggcacgaca tctttgcctt atctacctgt catcattcac
    19981 actcaacaag aggcgatgat ttcaggctgc aatggttttt attttggcaa cgagtcaccg
    20041 acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccgaa
    20101 caaaataccg agtgcattat tggtagcgcc ttttgtctaa tcatccacaa taatgacgtc
    20161 agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc
    20221 ttaatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata agcgccccac
    20281 gtttaaaaga gcattacgca acatcagtat gaccagcata tttatcacta tgatgctgat
    20341 ctggttgctg ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct
    20401 ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg
    20461 ccctgcagca actgaaacac tggcagcgct gggccagcaa ggccaatttt caactgcaga
    20521 agtgcgtgat aagcagcaaa atattctggc atcctggcat tacacccata aggaaccagg
    20581 cgatactttc agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat
    20641 tcgtcacaat ggtgaaacca ttggcgaagt acgcttaact gctcgcgaca gttcaatcag
    20701 ccattttatc tggttttcgc tcgctgtcct gaccggttgt attctgctgg catcaggcat
    20761 cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcgctga aaaatatcac
    20821 cgatgtcgta catgatgtgc gttctaaccg caatttttcc cgacgagttt cggaagaacg
    20881 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
    20941 gtggcagctt cgtttacagg ccaaaaatgc gcagctttta cgtaccgcgc tacatgaccc
    21001 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaacacgt tgatgaacaa
    21061 ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat
    21121 caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatcg caaaacggtt
    21181 agctgaattt ggcggactgc gacataaagc ataccgcctg ggcggcgatg aattcgcgat
    21241 ggtgctctat gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca
    21301 aatctttaat cgcccgtttg atcttcataa tggtcatcag accaccatga cattaagcat
    21361 tggttacgcg atgaccattg agcatgcctc tgcggaaaaa ttacaagagc tggccgatca
    21421 caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat
    21481 cgatatgata aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg
    21541 ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt
    21601 tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa
    21661 tgactacaaa ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc
    21721 ctcgacaggg ctaacgcatg cccgtatgga tggacacacc gacaactatg gtgaagacag
    21781 ttacaacgaa gccttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctatggg
    21841 tggacaaatt ccgcgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc
    21901 cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattactac
    21961 cccttaaaac acgagcaaac agataaaaaa agggccagcc aattggccag cccttcttaa
    22021 caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca
    22081 gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa
    22141 atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa
    22201 attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc
    22261 tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta
    22321 tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata
    22381 atgttgctca taatttaatc tcttatcctg ggtaaactga tatctcgggg gcttacgcca
    22441 tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg
    22501 cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc
    22561 ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg
    22621 gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc
    22681 cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc
    22741 gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc
    22801 cgattgacca ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc
    22861 gaccgcacac cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa
    22921 gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg
    22981 ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt
    23041 aaggacgatc gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccagctct
    23101 ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc
    23161 ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct tttaccgttt
    23221 atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga
    23281 ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat
    23341 ctttcaggtt tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcgacga
    23401 ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccatcaag tctttccagt
    23461 agtagtcacc ctcttcaagc tgaggcaact gcgatgaatc cacgacaatt tcacaattcg
    23521 tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat
    23581 tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc
    23641 agggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc
    23701 cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg
    23761 cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt
    23821 tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc
    23881 gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa
    23941 cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga
    24001 acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg
    24061 tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa
    24121 aattttactg atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc
    24181 atttagcgac caggaaatcc cggcggcatc atacccttca tgcttctcat catcttcgcc
    24241 attccgccct tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg tttcagaaga
    24301 cggttaacgt cctgcacctg cataccgcaa ccggcagcga tacggcgttt acgcgaacct
    24361 ttgatgattt ctggcttagc gcgctctttc atcgtcatcg agttgatgat ggcttccata
    24421 cgcaccagca ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc
    24481 ggcagtttgc ccatcaggct ggccatgccg cccatatttt tcatctggcg cagttgctca
    24541 aggaagtcat tgagatcgaa gccgtcgcct tttttcagct tgctggctaa tttctcagct
    24601 tgcgcgcggt caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg
    24661 agaatacggg aagcgatgcg gtccggatgg aacggctcca gcgcctcggt tttctcgcca
    24721 acaccgagga acttgatcgg tttgcccgta atgtgacgaa tagagagcgc cgcaccgcca
    24781 cgagcatcgc cgtcgacttt agtcagtacc acaccggtca gcggtaacgc ttcattgaat
    24841 gcttttgccg tattggccgc atcctgacca gtcatggcat caaccacaaa cagggtttca
    24901 accggattaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc
    24961 agacgaccgg ctgtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc
    25021 gccgcgttaa cgatatctac cggcttctga ccaacatcag aagggaagaa atcaacgccc
    25081 acctgctctg ccagcgtctc aagctgtttg attgccgccg gacgataaac gtcggcagaa
    25141 accaccagca ctttcttctt gtgcttctcg cgcaggaact taccgagttt accaacgctg
    25201 gttgttttac cggcaccttg caggcccgcc atcagcacga ccgcaggcgg ttgcgcagcc
    25261 aggttcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacaattttg
    25321 acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt
    25381 acgcgattga taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg
    25441 cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata
    25501 ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg
    25561 tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
    25621 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
    25681 tttcttattg ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc
    25741 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
    25801 cggcgcatgg ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa
    25861 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
    25921 ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
    25981 ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg
    26041 cccaatgaat acatcaccca tctggaagca acgcctggga tgctggtgca cattggctta
    26101 tcgctctttt cctatgccac gctgattatc gccgccctgt acgcgctgca actggcgtgg
    26161 attgattacc aactgaagaa caagaagctg gcgtttaacc aggaaatgcc gccattgatg
    26221 agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg
    26281 ctttgcactg gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggcc
    26341 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttatcatgaa
    26401 ggatggcgtg gacgccgcgt cgtctggttt aacgttgcgg gtgcagtcat tctgacactg
    26461 gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc
    26521 ccctggaaca catttctact actacgttga tcattattct gatcatcatg gtggtcattt
    26581 cagcctattt ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtctgcgac
    26641 atatggcgaa acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag
    26701 accgcctgat tagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc
    26761 taggcactat tgttgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg
    26821 tgctgacttt tgtcgtgctg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt
    26881 acccggaaaa agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga
    26941 tgccgctggt ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca
    27001 aaaccgatat cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcacg
    27061 aatcgcgctc acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg
    27121 aaaaaatgac cgttgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca
    27181 acgatgactg gaaatcgatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc
    27241 tctaccgtga ttcgctggac gacgccatca gtatgctgcg tgtgcgtgaa gcctggcgac
    27301 tcatgtcgga gaaaaaagag ttcaccaaag aaaccatgct acgcgccgcg gacgagatct
    27361 attttgtccc ggaaggtacg ccgctcagca cgcagttggt gaagtttcag cgcaacaaaa
    27421 agaaagtcgg cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag
    27481 atattctgga agagattgtc ggcgatttca ctacctcgat gtcgccaaca cttgccgaag
    27541 aggtcacgcc gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgagaaa
    27601 tcaacaaagc ctttaactgg catctacctg aagacgatgc ccgcacggtt aatggcgtca
    27661 ttctggaagc gctggaagag atccctgtcg ctggcacccg cgtgcgtatt ggcgagtacg
    27721 atatcgatat tctcgacgta caggataata tgattaagca ggtaaaagtt tttcctgtga
    27781 aaccgctgcg cgagagtgtg gcggagtaaa aaaacggccc ggcattcgca tgctgggccg
    27841 tgatttatta cgaaagcaga aattaagctt ttgccttcgc tacagtcacc atcgccgcac
    27901 gaatcgtacg accgttcagc gtataacctt tctgcataat gcccagtacg ttacctggcg
    27961 caacgtcatc agattccacc attgcgatgg cctgatgcac atttgggtcc agtgggacgt
    28021 tggtttcggc gatcacttca acgccaaact tacgcacaac atccagcatc gacttcagcg
    28081 tcagctcaat accttcaacc atcgcggaca tatccgggtt agctttatca gccacttcca
    28141 gcgcacgatc caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga
    28201 atttgtgagc tttttcaata tccagttcag tacgacgacg caggttttcc atttcggctt
    28261 ttacgcgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat
    28321 tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga agcttctggc tcaactgcct
    28381 caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct
    28441 gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct
    28501 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
    28561 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
    28621 cggcacccca ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
    28681 tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact
    28741 ggcacgctcg cggagattgg gcaactggca gatctcgcgg tagtcgttgg tggcgacggt
    28801 aatatgctgg gcgcggcgcg cacactcgcc cgttacgata ttaaagttat tggaatcaac
    28861 cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca acaattagcc
    28921 gatgtgctgg aaggccacta catcagcgag aaacgttttt tgctggaagc gcaagtttgt
    28981 cagcaagatt gccagaaacg catcagcacc gcgattaacg aagtggtact tcatcctggc
    29041 aaagtggcgc atatgattga gttcgaagtg tatatcgacg agatctttgc gttttcgcag
    29101 cgttccgatg gactgattat ttcgacgcca acaggctcca ccgcatattc cctctctgcg
    29161 ggcggtccta ttctgacccc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    29221 acgttgtcag cacgaccact ggtgataaac agcagcagca cgatccgtct gcgtttttcg
    29281 catcgccgta acgacctgga aatcagttgc gacagccaga tagcactgcc gattcaggaa
    29341 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat
    29401 tacagttatt tcaatacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt
    29461 tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata acagtaatgg
    29521 tttttcatac aggaaaacaa ctatgttggc acaactgacc atcagcaact ttgctatcgt
    29581 tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg agaccggcgc
    29641 gggtaaatct attgcaatag atgccctcgg cctttgtctc ggtggtcgcg ctgaagccga
    29701 catggtgcgt accggcgctg ctcgtgccga cctgtgcgcc cgtttttctc tgaaagatac
    29761 gccagcggcc ctgcgctggc tggaagaaaa ccagcttgaa gacggtcatg aatgtttgct
    29821 tcgtcgcgta atcagtagcg atggtcgctc acgaggtttt atcaacggta cagctgttcc
    29881 tctgtcacaa cttcgcgaac tgggccagtt gctgattcag atccatggtc agcacgccca
    29941 tcagttactc accaaacccg agcaccaaaa attcctgctt gatggctatg ccaatgaaac
    30001 ctctctgctc caggaaatga ccgcacgtta tcagttgtgg catcaaagct gccgtgacct
    30061 cgcgcatcat caacagttaa gtcaggaacg cgccgcccgt gcggaactgc tgcaatacca
    30121 attaaaagaa cttaacgaat ttaatccgca gcccggagag tttgagcaaa tcgacgaaga
    30181 gtacaaacat ctggcgaaca gcggtcaatt gctgactacc agccagaatg cattggcagt
    30241 aatggccgac ggtgaagacg caaacctgca aagtcagctt tacacggcta aacaactggt
    30301 gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaagc
    30361 taccatccag attgctgaag ccagcgatga actgcgccac tactgcgatc gtctggatct
    30421 cgatcccaac cgactgtttg aacttgaaca gcgcatctca aaacagattt cgctggcacg
    30481 taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac tggaagaaca
    30541 gcagcaactg gacgatcagg ccgactcaca agaaacgctc gcgctggcgg taacgaaaca
    30601 tcatcagcag gcactggaaa ccgcgcgcgc attacaccag caacgccagc agtatgcaga
    30661 agaacttgca ctgctgatca ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt
    30721 tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggtgccg atcgtattga
    30781 gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg
    30841 tggtgaattg tcacgcatcg cattggcaat tcaggtcatc acggcgcgta aaatggaaac
    30901 cccggcactg atttttgatg aagtggatgt agggattagc ggtccaacag cggcagttgt
    30961 cggcaaactg ctgcgtcaac ttggcgaatc aactcaggtg atgtgtgtta cccacctgcc
    31021 acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat
    31081 gacagaaaca cacatgcaat ccctggataa aaaagcgcgg ttacaagagc tggcgcgcct
    31141 gcttgggggc agtgaagtca cacgtaacac actggcgaat gcgaaagaac tgcttgcggc
    31201 gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag
    31261 caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt
    31321 actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag
    31381 cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc
    31441 ctgacatcaa ccaggggaat tatctgaccg ctaacgacgt atccaaaata cgcgttggca
    31501 tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat ccatttggta
    31561 cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa
    31621 cgctgacgct gacctttaat agtagcgggg tgttgaccaa tattgataat aaacctgcgc
    31681 tgagtggtaa ctaatcataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca
    31741 ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc gcagctcttt
    31801 cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct
    31861 cagttttgcc ggacggctat aaatgccgac tttgttttta gttaaatcga tatcggtacg
    31921 caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcaccct cctgcagcgt
    31981 cacacgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc
    32041 tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta gccgccagtt
    32101 ctttaaacac gcggccaaag gcgagttcaa tcaacttatt ggtaaactca aaatcgagat
    32161 gaaactcgat acggcacgcc tcctgactca gcggcgtaaa cttccatcca ccgatcaatt
    32221 tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc aactggttgc
    32281 gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca gtcatctgcc
    32341 caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga ggataagact
    32401 gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt gcggtccggc
    32461 taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc atttatcctg
    32521 ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa gatagagcct
    32581 tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg attcacgacg
    32641 cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct taacaagcgc
    32701 gcccggcacg aatactttat cgaagaagag ttcgaggcgg gacttgccct gcaaggctgg
    32761 gaggttaaat ccctgcgtgc aggaaaagcc aatatcagcg acagctacgt ccttctgcgt
    32821 gacggagagg catttctgtt tggcgctaac atcacgccaa tggctgtggc ctccacgcat
    32881 gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga actggactca
    32941 ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct gtactggaaa
    33001 aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta agaaacagca tgataaacgt
    33061 tcagatatca aagaacgcga atggcaggtg gataaagcgc gtatcatgaa aaacgcccac
    33121 cgttaaacct gcactgcaat tattgatccg ctcctcaccg cgcctccctc tccggcggcg
    33181 cgaatgcaca tcttattggc tatcacatcc gacccaaatg ttgccatccc attgcttaat
    33241 cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat tgaggctggt
    33301 catggcactc atgaatctgg tatacttacc gttacacatt ggggctgatt ctggattcga
    33361 cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa
    33421 aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct
    33481 ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg
    33541 cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc
    33601 gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggac
    33661 gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga ttggacggtg
    33721 taaggacaac accaacaaaa acaggaagtt agcagtctca gcaagacacc gaccagacgg
    33781 tgaggagaca taaaaggata cgcaaaggag ccgcggctcc tggtgatatg aaagcccaca
    33841 gatgtgggct ttttcgttga tggtcagaac gaccagttca caccagccac cgcgttccac
    33901 ggggattcca caccggcacc atggctatac cccaccccga gatgcccgct taacgaactg
    33961 ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgaca
    34021 aaattaccgt cattattcac tttcacccgg ttatcatcga caaattcttt gcgcacagcc
    34081 gccttcagcc acggctcaac ttccatgccg ttccccagac gcatgttgta actcagcgtt
    34141 gcgcccagtt cacgatatat actacgggta tcgactgatt tcgattccat gccattggat
    34201 aaatgatatt cggggttatc agcggtgaac cccgttaacg atgcatacgg cgtcaggttc
    34261 cagttaccat cggtaaatcg catcccggtt tcaatgtgac cgcccagccc gttgctgcga
    34321 taactgccgt tggcggctcc accgctgctc attttaccgg ctacgttact ttcaaagcgg
    34381 ttcagcttca cgataccgtc cagatagaaa ccactttcat gttcccagct ggcatagccc
    34441 cccagagaat aactgcccac actgccatgc cctccacgat caaaaaagat atgtgtatgg
    34501 gaataaccca taaaagcgcc cagcgtcgca atcccctcag gaatatcatt acggctatcg
    34561 atccccaccg tcattccggt cagcgtctgc tcaaacccgg cacccgcatc ggtggtgaca
    34621 ttattacggg tgttatacgt cgtcccccag acattattgt tgtgtggact cgctttcatt
    34681 atgttcaacc gctcgcgaat actgtttagc tcagcatcaa agaccaacgg taatgttgct
    34741 gccatattga gtacggctgc cgtagaaggc gtgatgcgtt tctccgggac gggtgtcggc
    34801 gttggatcgg gagtcgggtc tggtttcgga tccggttttg ggtctggttt tggatccggc
    34861 ttcggatttg ggttggggtc cgggttgggt ttgacatcat tggtcaggtt ccagttgctg
    34921 ttgccatcgc ttttcaggac atactcatag gtcccaagat caacgaaacc gccggtatta
    34981 cccagcgtaa acgaagcatc ccctccccct gttttcacca gcgtcatcgc gtcgtcagac
    35041 tgagggctga cgccggtatc ctgaacaaag attttaaaat tgccagtggc gttgttgttg
    35101 acgaccagtt gatcgccccg ggagcctgca acgttggtat gcaggtagaa attaccgctg
    35161 ccggaaagtt cactggtggt cagcgtattg tagataccat tagttgtggc aggcgctgct
    35221 gtttgtgctg ataaataaac atcaccgtcg ttgaggagca aactatttac tctatattcg
    35281 cagttgctgg tgcctgcaca ggaattattg ctgttaagcc agacactgcc ccggctggca
    35341 gccgtcaggt cggcaagcgt cgtatcgaca ccatttccca gagttaatgt cgcgctatcg
    35401 gtaatccgga ccgccccttc aagttttact ggcgtaacat tatcccgtgg cgtcattaac
    35461 gacaggcttc ctgtcgcgcc actgaccgat gcatccgcca gcgtacctgc gtagacgatt
    35521 gccgtcccgc cagcaacctg tagatcttct gcccgggcca gaacctgaaa ctcatctttt
    35581 gaccgaccga gggtatattg cccaccagaa ttaacctttg tggcgaagtc ctgccccagg
    35641 ttttgcattg ccccaccgtt gccaaccgtg gaatcgcgag cttcggtacc tgctaatacc
    35701 aataaattac cgccattttc cagcagtaca ttggtcgcta aatatccggc aatggaaaag
    35761 gtgccgtact ggtgcgtacc gctgatttca ataccgttag ccgtgctcgt ctggagagcg
    35821 gcaccgctgt tctggacgat atctgtcgct ttgccattat cgttgactgt cagcgttccg
    35881 ccttcattga tcgttgtttt tattgcctct ccgttagctg aaactgtttg tattccgccg
    35941 tcgttaattg ttgtctcatt tgccacaccc tcgacgattt gttcaccgcc agtgagcgtc
    36001 gtgccagtcg cagtggcttt tgttttgacg atctcccgac cgcccgcatt gacctgtgtt
    36061 ttggcggaag tggtgtctga ctccacggtt aacacgccgc catttgtcag caggatattg
    36121 ttcgccgcac cctgctcgat gctgaacgcg acgccatcag cgcgtgtccc tgtgacccgc
    36181 gtcgccctgg tggttgcaac caacgcgccc tgactactct gctgtatccc cgttgcgctg
    36241 cctttctccc gcacatcgag tgtgccgccg tcattaagca ccgagttttc agccagaccg
    36301 ccctcattaa ctacctgtga accgccatta ataatggaac cttccgctgt cccgtttgcc
    36361 ataatttgtt gtaagccaga gacgatatcg gtattgatcg ccttaccata attctgaacg
    36421 gtttgtgtgc cgccattgat gtgcgttttg tctgttgacc caccatcaac aatttgttca
    36481 ccactttcaa tatttgcatc agtggctaaa ccatataccg tttgcttgcc acctttgata
    36541 tttgctttat cagaagtggc actggcatat attgtttggg tgcccgcact attgagtaca
    36601 gtgccaacat cttttccata aacatccatt ttgccgttgg cattaataat cgtatcaact
    36661 gcccgggaac cagtgacgac tgttaatgag ccagcgtttt ccagcactac atttttagct
    36721 tctgaattcc tgatgtagaa agcatcacca taactgttgg ttccttcgat aagggttccg
    36781 gaagtcgtgg aagcaattaa tgcgccgccg gattgttgct caacatgctt agcctcacca
    36841 ccgtcctgaa cctccagaac gccaccatta ttaagtctgg tgctatctgt tttagcctcc
    36901 ttctggacaa tcagcttacc gccagcatca atggtagtat ttttcgccga ggttttagcc
    36961 attaccgtca gttcgccggt attttccagc acaacataat tagcctcacc tccggtaata
    37021 gtgaagtgag agagcttatt gtatccttcg atatcagtcc ctgcgcccgt gttggcaact
    37081 aaagcactgc ccgtctcctg gttaacccca tgtgcaatac cgccggtata gacaatcagc
    37141 gagcctccgg cgctaatatt gctgccagtt gccgtaccat ctttctcaac aacctgccgg
    37201 ctcccagagg atatatttgt cgtgtcagct ttcccgccgc tcttaatatt ttgtgttccg
    37261 ctgttgatat tggtgcccgt ggcaatacca taattattaa tattctgtgt gccaccatta
    37321 attatggtat ttgtcgcgtt tccggcaaca tccataacgc cgccattatc tattcgggtc
    37381 gcatcagctt tagcattcgt taaaactgac attgttcctt tatctttaat aatcgtctta
    37441 tttgccgaac catatgcgtt tatgtctaaa tgaccaccgt tttccagcaa cacattgtct
    37501 gccacgttat tgtggatgga gaatgcccct tcactattcg taccgctcac cgtcaaatcg
    37561 taagtcgtta ctttcaaagc ggctccatcg tgctgagtaa catttgttgc cataccacct
    37621 ctaacatcaa gcacgccacc ggaataaatt tcaataacat ccgaggtgct agtgtaatca
    37681 acaatttgcg taccaccaga atagatctga gtattttttg ccgttgactt actatccaga
    37741 gactgagttc caccttcaat cgtcgtgtcc agcgcacggc tctcatatac tctttgctca
    37801 ccgccatttt taatggtggt tgtttctact gtgctctgtt caacatactg tcgaccacca
    37861 tttatggttg tgttcgtcgc cagacttcct tgcgtgatgt cctgataacc ggacttattt
    37921 atcgttgtac catcaacatg cccctgagtt gttactgtct gccctccacc atcaatgatg
    37981 gttccattcg cagtcccccc acttatgtgt gaattaccac ccttaattgt cgttccattg
    38041 ctgataccac ctgtataaac gtcctgattg ccactctcga ttattgtacc tgtggaaata
    38101 cccccgtcat gaattgactg tctgcccccg ttaatggttg tattattagc ctgccccaca
    38161 aaattgttat gacctcctac atcttgatat ccacccgatt caataagact tccattagat
    38221 atcccgccat aaacattctg ctggccatgg ttgataatat gagtgttatt tgttgtacct
    38281 cgttcatcta ctttttggct gccatctaca gtctcatcgt ttaccacacc aataacatca
    38341 ggagtgaagg ccgccatccc gggcggggca tatatcaagg cagatatcaa taaggaaagt
    38401 actgagcgac gacaataata gggactggtc ctgttcataa atttcatcct ctgaaaagtg
    38461 aatactgagt agcgtttaag cgaccttagc tttgctgcaa catcagccca caggcaccag
    38521 accaggggat tcatcctgaa gagacagcgc aagtgtattg tgttcaccgc tcatcaaaga
    38581 catcatgatg aaatgatgat attccgcata agaatgaggc attttttaaa cgcagtgcgc
    38641 tgaagtgtgg ttggataaaa aagtcaatct attcaggaaa cacgggcgta ttcttttctt
    38701 tcgacaatca ggcagtcgga aaaataaaat gatttacata atcgtttctg atgaatatct
    38761 tctgctcaca taaaaatcac acaataactc tgagatcgca gattgtttta cttttacagc
    38821 ttccgtcccc ccattgttgg gcaaatatag attgggccag agcacgaaag ttaataccac
    38881 gttcgcacag ctcctccaac agcacgacaa gatgccacat actgcgcccc agtcggttca
    38941 gtttacagac tagcagtgcg tcccctaccg ataatgtcct gagccgtttg ttctgtccgg
    39001 gcctgctgcc acggttatgc gctgaagtgg taactctgag cacgcagttc ttcagtcagg
    39061 cgtggtgcac cgtcacccta ttattgctgg gtaagatcaa aaaactttca agcagctaag
    39121 gaaagttgaa ccagacatta gatgaaatat ttcaaccaat tacagcacca gttcagacac
    39181 cgccaactca tcaaataaat caaggggtta cgtgaaagcg tagccccttt tactttggta
    39241 gtgacagcaa aatggatgca gtgtgaagaa taatcccgtt tactcaatca ataatacata
    39301 ttatttcaat ctacgttatt atttctttgt aaaaatcgcc atttatttat cattgaaaac
    39361 tgcttttaga actttataca aggggactag tcacaacagg actattctca acgggctcat
    39421 cctcagagga actatcatca aagtcatcat ccatagatga aataccatcg aatggtgcca
    39481 cgcccgtgat tagttttatt ttattattac gatcagtcaa gactccattt aaaccatttt
    39541 cgctgacgga ttttaataat ttatcataac tcttattgtc atcaggcaca ttaaaaatac
    39601 acggagtatc aacatcaaac aatgacgttc cccagttcac atatttaata tcatagttac
    39661 tgaagttcta tccagaaaag aagcatccct taaaatccaa tccacctaaa ttatataaac
    39721 caccctcttc tttttggaga gcaatgttaa ttttggctat ctcccggaca tcatcgccat
    39781 ttttgtattt gaataccgtt tcaagatgtt ctccagacag cttgacttct ggaaataatt
    39841 tgaaattaaa ccctacatta ttatgtgtca acgtagatga acaaaagatg gaaaaagctt
    39901 gcagtgctga attatagcta tcgattttat cttgaggctg cgctcttggt aaaaacttat
    39961 agcaacactc ataaaaatta agtagaaact ctgatgacct tccattttta tcaaataata
    40021 cccgctgaaa atgctgcaca aacttattcg ttttttcttg aatatctacg gtgtgttcct
    40081 ttgactctga caaagatgaa gcctgttgaa gtgcattgct catctggtta atgaacatgt
    40141 gcatttcttt aaatttatct ccatagtgac ctttaacttg ctgcgagttt ttagctacag
    40201 gtgaaatctt atctatttgg ttgtcatata aattatgtga ttccatacag tcatttggcg
    40261 gcgtttctga aataacacta cgtgttctac ctggctgaag tgaagcaaca tgaaaatcac
    40321 tttttattat attatcgaaa agggcctgtt catttctttt agcggattga acatactgat
    40381 caatattatt ttctctcgat aatttgttat tttcagaaaa cacattttct ttaatctccg
    40441 gttttaattt aatgagaaga tcttcccact cctacttgct taaacatact acgtcaggct
    40501 caccatcagt aactaactct tcattattat caaagtgtac gttgaatact tttaagttat
    40561 tttcatcaac atcatattta atacgtttta attgttctcc agctcccata atgacaaagg
    40621 cgttgccttc ttgatatata cattcagaca ttattttttg taaagtttca agagcaccgc
    40681 gatacatccc tgaggcagct tccttacaaa ttaaatttat aatactataa gctagtagct
    40741 ccatggggtt ggaagaattg ttttcattac aacttccact acccaaaact gtattaacgt
    40801 tatgaccatt acttttacta actaacattg tctattcctc agttaat
//
LOCUS       DAZGTJ010000035        39972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000035 DAZGTJ010000000
VERSION     DAZGTJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.035"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..205)
                     /locus_tag="ACHK8E_002321"
     CDS             complement(<1..205)
                     /locus_tag="ACHK8E_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020236720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP0344580.1"
                     /translation="MRDLKDKIYFYTEGEFLEMLEEIVNATSKDKSLKGKKLEKYLDT
                     LVDHFIKITEHPKKGDLIFYPNSQ"
     gene            complement(211..1992)
                     /gene="tssD"
                     /locus_tag="ACHK8E_002322"
     CDS             complement(211..1992)
                     /gene="tssD"
                     /locus_tag="ACHK8E_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP0344581.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECITVDYDYILCKHLIVNTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFAQPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDLRKLPGVVTGRGVPLSLGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFRIVTPRLHDEIHYRR"
     gene            2518..3891
                     /gene="aroP"
                     /locus_tag="ACHK8E_002323"
     CDS             2518..3891
                     /gene="aroP"
                     /locus_tag="ACHK8E_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP0344582.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(3934..4788)
                     /gene="ampE"
                     /locus_tag="ACHK8E_002324"
     CDS             complement(3934..4788)
                     /gene="ampE"
                     /locus_tag="ACHK8E_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP0344583.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(4785..5336)
                     /gene="ampD"
                     /locus_tag="ACHK8E_002325"
     CDS             complement(4785..5336)
                     /gene="ampD"
                     /locus_tag="ACHK8E_002325"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP0344584.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            5424..6317
                     /gene="nadC"
                     /locus_tag="ACHK8E_002326"
     CDS             5424..6317
                     /gene="nadC"
                     /locus_tag="ACHK8E_002326"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP0344585.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            6520..6960
                     /gene="ppdD"
                     /locus_tag="ACHK8E_002327"
     CDS             6520..6960
                     /gene="ppdD"
                     /locus_tag="ACHK8E_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP0344586.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            6970..8355
                     /gene="gspE"
                     /locus_tag="ACHK8E_002328"
     CDS             6970..8355
                     /gene="gspE"
                     /locus_tag="ACHK8E_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP0344587.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            8345..9547
                     /gene="hofC"
                     /locus_tag="ACHK8E_002329"
     CDS             8345..9547
                     /gene="hofC"
                     /locus_tag="ACHK8E_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP0344588.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(9582..10625)
                     /gene="guaC"
                     /locus_tag="ACHK8E_002330"
     CDS             complement(9582..10625)
                     /gene="guaC"
                     /locus_tag="ACHK8E_002330"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP0344589.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(10781..10825)
                     /locus_tag="ACHK8E_002331"
     CDS             complement(10781..10825)
                     /locus_tag="ACHK8E_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344590.1"
                     /translation="MIIKTLKMSARKRN"
     gene            10850..11470
                     /gene="coaE"
                     /locus_tag="ACHK8E_002332"
     CDS             10850..11470
                     /gene="coaE"
                     /locus_tag="ACHK8E_002332"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP0344591.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            11470..12213
                     /gene="zapD"
                     /locus_tag="ACHK8E_002333"
     CDS             11470..12213
                     /gene="zapD"
                     /locus_tag="ACHK8E_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP0344592.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            12223..12420
                     /gene="yacG"
                     /locus_tag="ACHK8E_002334"
     CDS             12223..12420
                     /gene="yacG"
                     /locus_tag="ACHK8E_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP0344593.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(12622..13020)
                     /gene="mutT"
                     /locus_tag="ACHK8E_002335"
     CDS             complement(12622..13020)
                     /gene="mutT"
                     /locus_tag="ACHK8E_002335"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP0344594.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(13080..15785)
                     /gene="secA"
                     /locus_tag="ACHK8E_002336"
     CDS             complement(13080..15785)
                     /gene="secA"
                     /locus_tag="ACHK8E_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP0344595.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(15847..16359)
                     /gene="secM"
                     /locus_tag="ACHK8E_002337"
     CDS             complement(15847..16359)
                     /gene="secM"
                     /locus_tag="ACHK8E_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP0344596.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(16590..17507)
                     /gene="lpxC"
                     /locus_tag="ACHK8E_002338"
     CDS             complement(16590..17507)
                     /gene="lpxC"
                     /locus_tag="ACHK8E_002338"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP0344597.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(17608..18759)
                     /gene="ftsZ"
                     /locus_tag="ACHK8E_002339"
     CDS             complement(17608..18759)
                     /gene="ftsZ"
                     /locus_tag="ACHK8E_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0344598.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(18820..20082)
                     /gene="ftsA"
                     /locus_tag="ACHK8E_002340"
     CDS             complement(18820..20082)
                     /gene="ftsA"
                     /locus_tag="ACHK8E_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP0344599.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(20079..20909)
                     /gene="ftsQ"
                     /locus_tag="ACHK8E_002341"
     CDS             complement(20079..20909)
                     /gene="ftsQ"
                     /locus_tag="ACHK8E_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP0344600.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(20911..21831)
                     /gene="ddlB"
                     /locus_tag="ACHK8E_002342"
     CDS             complement(20911..21831)
                     /gene="ddlB"
                     /locus_tag="ACHK8E_002342"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0344601.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(21824..23299)
                     /gene="murC"
                     /locus_tag="ACHK8E_002343"
     CDS             complement(21824..23299)
                     /gene="murC"
                     /locus_tag="ACHK8E_002343"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP0344602.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(23353..24420)
                     /gene="murG"
                     /locus_tag="ACHK8E_002344"
     CDS             complement(23353..24420)
                     /gene="murG"
                     /locus_tag="ACHK8E_002344"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP0344603.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(24417..25661)
                     /gene="ftsW"
                     /locus_tag="ACHK8E_002345"
     CDS             complement(24417..25661)
                     /gene="ftsW"
                     /locus_tag="ACHK8E_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP0344604.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(25661..26977)
                     /gene="murD"
                     /locus_tag="ACHK8E_002346"
     CDS             complement(25661..26977)
                     /gene="murD"
                     /locus_tag="ACHK8E_002346"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP0344605.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(26980..28062)
                     /gene="mraY"
                     /locus_tag="ACHK8E_002347"
     CDS             complement(26980..28062)
                     /gene="mraY"
                     /locus_tag="ACHK8E_002347"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP0344606.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(28056..29414)
                     /gene="murF"
                     /locus_tag="ACHK8E_002348"
     CDS             complement(28056..29414)
                     /gene="murF"
                     /locus_tag="ACHK8E_002348"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP0344607.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(29411..30898)
                     /gene="murE"
                     /locus_tag="ACHK8E_002349"
     CDS             complement(29411..30898)
                     /gene="murE"
                     /locus_tag="ACHK8E_002349"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP0344608.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(30885..32651)
                     /gene="ftsI"
                     /locus_tag="ACHK8E_002350"
     CDS             complement(30885..32651)
                     /gene="ftsI"
                     /locus_tag="ACHK8E_002350"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP0344609.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(32667..33032)
                     /gene="ftsL"
                     /locus_tag="ACHK8E_002351"
     CDS             complement(32667..33032)
                     /gene="ftsL"
                     /locus_tag="ACHK8E_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP0344610.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(33029..33970)
                     /gene="rsmH"
                     /locus_tag="ACHK8E_002352"
     CDS             complement(33029..33970)
                     /gene="rsmH"
                     /locus_tag="ACHK8E_002352"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP0344611.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(33972..34430)
                     /gene="mraZ"
                     /locus_tag="ACHK8E_002353"
     CDS             complement(33972..34430)
                     /gene="mraZ"
                     /locus_tag="ACHK8E_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP0344612.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(35032..36036)
                     /gene="cra"
                     /locus_tag="ACHK8E_002354"
     CDS             complement(35032..36036)
                     /gene="cra"
                     /locus_tag="ACHK8E_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP0344613.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(36118..36204)
                     /locus_tag="ACHK8E_002355"
     CDS             complement(36118..36204)
                     /locus_tag="ACHK8E_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP0344614.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(36216..36707)
                     /gene="ilvN"
                     /locus_tag="ACHK8E_002356"
     CDS             complement(36216..36707)
                     /gene="ilvN"
                     /locus_tag="ACHK8E_002356"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0344615.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(36710..38434)
                     /gene="ilvI"
                     /locus_tag="ACHK8E_002357"
     CDS             complement(36710..38434)
                     /gene="ilvI"
                     /locus_tag="ACHK8E_002357"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP0344616.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            38553..38597
                     /locus_tag="ACHK8E_002358"
     CDS             38553..38597
                     /locus_tag="ACHK8E_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344617.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(38752..39696)
                     /gene="leuO"
                     /locus_tag="ACHK8E_002359"
     CDS             complement(38752..39696)
                     /gene="leuO"
                     /locus_tag="ACHK8E_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP0344618.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9746 a        11016 c         9652 g         9558 t
ORIGIN      
        1 cttgggaatt aggatagaaa atgagatctc cctttttagg atgttcagta attttaatga
       61 aatgatccac taaggtatcg agatattttt ctaatttttt cccttttagg gatttatctt
      121 tgctagttgc atttactatc tcctcaagca tttcaagaaa ttcaccttct gtgtaaaaat
      181 atattttatc ttttaaatca cgcatataca ttatctcctg tagtgaattt catcatgtag
      241 tcggggcgta acaatcctaa aattatcaat atcatacact ccaccaccat gttctatagc
      301 aactacatga tgtatctgga atttcttaac tatctcattt ggtccgtaat aatacccctc
      361 ctcagggaca tagggggaat atccttgact aacgttattc tgattacttt ttataaactg
      421 cgccaataac actgagtctt tactcacctc cagccacagc gcctcgcgaa actcatcaaa
      481 ggttttaaac tcccgtccgc gcaatttatc agcaatatgc gccgggattg gtgcgccgtt
      541 gccgttattg gataccgaca tatccagcca gcgagttcct agtgaaagcg ggacgccgcg
      601 ccctgttaca acaccaggca gctttcgcag atcctccttc aggtaaacat agatgggctt
      661 aataccggat cccgccggaa aaaccagaat ataatcgttg aattctttcg gatcaggaac
      721 cgggaatggc ggcgtggtat aggtatcggt gccgtccgga atcggcgtca ccagaatggt
      781 gggctgatcc agcggcggcc tgtcgttacc ggtatggccc ggaacattcc catgttccgg
      841 attttccggc gtccaggtaa tcagggggcc atcgacatcg tgagcggggg taaattcata
      901 acgctgcgtc tgctcgttcc actgcatgtt cgccacgcgg acgttttcat acggcgtacc
      961 ttcacccgta tgaacaccat acacctgagg cttgccgtga atgtcagttc cccagaaaaa
     1021 acgtacccgg gtggtggcgg tactgcctgc tagtgctttc tgccgcagtt cttcctcata
     1081 ccagaactca tcacgtccgg gcacgcgagc actgccctcg cccgcagagg gaatatacaa
     1141 agcagatgcc aacgcaccga tgtaagagcc gcgggttgca ctgagtatgc cactgagcga
     1201 ccattttccc catatttgag ccgccaacac actacccgga aacagtagca tggtttgagc
     1261 ttctgcgggt tgcccgacat cgttagcctc gggatggtgg tatccacagc agtcatcaac
     1321 cactggctga gcaaaaatcg ctacttgacc gaagttttca gcgggttctt ctgccgttcc
     1381 agcatctgta catcccttct ccttcagaca ggattttgca tagaccgggt taaccgctgg
     1441 tgaagagagt ggaaggttaa caactggtag aggttcaggc ttaagattac tggcaggtgg
     1501 gcttttaggc ggaataaata agctattata gccagcagga aaggccaacc agtcaaattc
     1561 cgtattgact attagatgtt tacataatat gtagtcataa tcgacagtta tacattcggt
     1621 aggaagatta ttgtcagaaa tgtttacatg aatagcagtt aatgaagcat ttcttagctg
     1681 aatataataa taccgttccc agcgcccgct tttatttata cgatacaaat cgatttcaat
     1741 aaataaccgc tcattctgat tgatggcatt agacagtagc ggtgagcttt tatcaatgag
     1801 tttgcaaaat tgtagcccct gtagattaac gcctttacca gtactggtaa tggcgttggt
     1861 gagtgcaaag gcaaaaattt catcttcatg gccaacctgc cagcaattac caaccgacgc
     1921 atacgtccca cagcctgaag aaatatagcc ctgcttctca ccgattattc tcaggtaaac
     1981 aatatcgctc actttatttc attccagttt atccttaact tatttgcatt aaatagaaaa
     2041 cttaattctc gatcaataag aaacgctttt aatgaaagat tatttcaatt acttaacgtt
     2101 ttcacttagc tcgcaatatt atatttaaat actcgcgtta caaacccatt gtattattaa
     2161 catctattta ttcttatatg accttttgta agattattgt gctggtggct aaggcatagt
     2221 tttcagcagg cagacaaatc cggcatatag ctagttaaaa cagtatgtta tcagacttaa
     2281 agtatctatt aaatgtgatg aaaaacttac aattgctttt tttttatcca aaaaatcaac
     2341 gcatctacat aaagcaggtg cattcccggg cacataccat tatcttgtga tagttaaaag
     2401 tagcattatc ataattcagt tacatgaaac gtaaagaaat caatacaaca aacggaattg
     2461 caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg
     2521 atggaaggtc aacagcacgg cgagcagcta aagcgcggtc ttaaaaaccg ccatattcag
     2581 cttatcgcgc tgggtggcgc gatagggacc gggctattcc tgggtagcgc ctccgtaata
     2641 cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg
     2701 atcatgcgtc agctaggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac
     2761 tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta
     2821 ctgtacgttt tagttgccat ggctgagctg actgccgtgg gtaaatacat tcagttctgg
     2881 tatccggaaa tcccaacctg ggtttctgcc gccgtgttct ttgtggtgat taacgccatc
     2941 aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt
     3001 atcgcggtgg tagcgatgat catcttcggc ggctggctgc tattcagcgg caacggcggc
     3061 ccgcaggcga gcgttagcaa cctgtgggat cagggtggtt tcctgccgca cggcttcacc
     3121 gggctggtga tgatgatggc gattatcatg ttctctttcg gcggtctgga actggtgggg
     3181 atcaccgcag cagaagctga taatccggag caaagtatcc cgaaagcaac taaccaggtt
     3241 atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc gctgatgcca
     3301 tggacccgcg ttactgctga taccagtccg tttgtactga tcttccacga attgggcgat
     3361 acctttgtgg ctaatgcgct gaacatcgtg gtactgactg ctgcgctctc cgtgtacaac
     3421 agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg caacgcgcca
     3481 aaagcgctgg cgtctgtcga caaacgcggt gtaccggtta acactattct ggtgtctgcg
     3541 ctggttacgg cattgtgcgt gctgattaac taccttgcac cggaatccgc attcggactg
     3601 ttaatggcac tggtggtatc tgcactggta atcaactggg cgatgattag cctggcgcat
     3661 atgaaattcc gtcgcgccaa gcaggaacaa ggcgtggtaa cttgcttccc tgctctgctt
     3721 tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg
     3781 atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt
     3841 atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac
     3901 gccctcaccc gtgcagggtg agggcaataa tctttacacc aacgccccgt aaatcgtcag
     3961 tagcgcaatt accaccagga ccacgaacga ggttttcttc gccatggaaa ccgctgcctt
     4021 cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgtg ccagacgtgt
     4081 taatacctga tattgcgaag tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc
     4141 tttctcccca tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc
     4201 cagtacatga agcacggcat caatgccgga ctgtaaacga tgatgcggcg tctggtaacg
     4261 cgccagccag tattgccatg cacgcaaaaa cgcatacccc atcagcgtaa cgggtcccca
     4321 ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt taatccacag
     4381 caatgcattt tgcagctcac gcaaatactc acgttcatcg cagcctgccg ggacgccgtg
     4441 aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc
     4501 tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat
     4561 cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag
     4621 taaaaaagtc acgcccatcg caataatggt catgcctaat gtgcgcccga gagaaaaatg
     4681 tttcacccgc cgaaagaacg cttcaagacg atgatcaatc tgccagtgct cgcccaactt
     4741 aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg
     4801 ctgaccagtg cacgaaaccg tgcccaatca aatgcagggc cgggatcggt tttccgatcc
     4861 ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat ctggatagcg atcaatcagt
     4921 gcccgcgtaa ccgccacaag ctgttgatac tgcgcatcgg tatacgccag cgtatcggtg
     4981 ccttccagct caatcccaat agaaaaatca ttgcagcgtt cgcgcccctg atactgagag
     5041 actccggcat gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcgcgg
     5101 cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgcgcc
     5161 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
     5221 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg
     5281 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
     5341 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
     5401 cgttagctat ctggagtttt aacatgccgc ctcgccgcta taaccctgac acccgacgtg
     5461 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
     5521 aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa
     5581 actctcgctc tcatgccaca gtgatcacac gcgagaatgg cgtattttgc ggcaaacgct
     5641 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
     5701 atgacggcga tgtcatcaac gccaatgaac ccttgttcga acttgaaggc ccatcccgcg
     5761 tgctgttaac gggcgaacgc actgcactta attttgtgca aaccctttca ggggttgcca
     5821 gtaaggtacg ccactatgtc gaattactgg agggcaccaa cacgcagttg ttggatacgc
     5881 gcaaaacctt acccggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag
     5941 cgaatcaccg cctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgcct
     6001 ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcacccagat gcgccagtag
     6061 aagtcgaagt agagaatctg gaagaacttg atgaagccct gaaagcaggt gccgatatca
     6121 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
     6181 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa
     6241 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtgcaggca ctcgaccttt
     6301 caatgcgttt tcgctgattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc
     6361 cctttcccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgtaata
     6421 aaaaactccg gaagctgctt tcccgtttcg ttttttgccc ctcgcttgtg ctgatggcgt
     6481 ttgccaaagt agcgccaacc aaatcaagga gcgaaacaaa tggacaagca acgcggtttt
     6541 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagcgc cattggtatt
     6601 cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg
     6661 ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
     6721 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
     6781 gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg
     6841 acaccgggtt gggataacgc aaacggcgtc accggctgga cgcgcaactg caatattcaa
     6901 agtgacagcg cattgcagca agcctgcgaa gatgtctttc gctttgatga cgccaactaa
     6961 ggagcggcaa tgaatattcc acagctcacc gccctgtgtc tgcgttatca gggagtcttg
     7021 ctggatgcca gcgaagaggt ggttcatgtt gcggtggtcg atgccccttc gcatgagcta
     7081 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
     7141 caaatggaag gtcacgccag tcgcacccaa cagacattgc ccgtagctat tcatgagaag
     7201 catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga acaacgcgcg
     7261 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggt
     7321 gtattgcatc ctttaccgga tgttttaccg gatgccggag tcgcattaac cgccagatta
     7381 aaagtgctgg gcaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
     7441 gtcgaactgg caggaaacgc catctcattt cgtattgcga ccttaccatg tcggggtggt
     7501 gaaaaggtgg tattaaggtt gttacagcag gtgagtcagg cactggatgt caacacgctt
     7561 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
     7621 gtgctggtaa caggccctac aggcagcggc aaaacggtca cgctttatag tgccctgcaa
     7681 acgctgaata ccgctgacat taatatttgt agcgtcgaaa atccggttga gatccccata
     7741 gccggactaa accagacgca aatccatccg cgtgccggac tcacctttca gggcgtttta
     7801 cgtgcgttat tgcgccagga tcctgacgtc attatgatcg gagagatccg cgatggcgaa
     7861 acggcagaaa ttgccattaa cgccgcacaa acgggtcatc tggtattgtc caccctacac
     7921 accaactcca cctgcgaaac gctggtacgt ttacagcaaa tgggagtcgc ccgctggatg
     7981 ctctcatcag cgcttacgct ggtaatagcc cagcgtctgg tacgcaaact ttgcccacat
     8041 tgtcgccgac agcaagggga gccaatccat attccagaca atgtatggcc gtcgccgctg
     8101 ccccgctggc aagcacccgg ttgtgtacat tgctaccacg gtttttatgg tcgcacggcc
     8161 ttatttgaag ttctgcccat aacaccggtc atacgtcagc ttatttccgc taataccgac
     8221 gttgaatcgc tggaaacgca cgcccgacag gcgggtatgc gaacgctttt tgaaaacggc
     8281 tgtctggccg tggagcaagg cttaaccacc tttgaagagt taatccgcgt attggggatg
     8341 ccgcatggcg agtaagcaac tctggcgctg gcgtggcata accggcgacg gcaatgcgca
     8401 agatgggatg ctatgggcag agagccgtgc tttgctgctt atggcactac agcaacagat
     8461 ggttacccca ctaagcctga agcgaatcgc catcaattct gcgcagtggc gaggagataa
     8521 aagcgcggaa gtcattcatc aactggcgac gctactcaaa gccgggttaa cgctttctga
     8581 agggctggca ctgctggcgg aacagcatcc cagtaagcaa tggcaagcgt tgctgcaatc
     8641 gctggcgcac gatcttgaac agggcgttgc cttttccaat gccttatttc cctggtcaga
     8701 ggtatttccg ccgctctatc aggcgatgat ccgcacgggt gaactgaccg gtaagctgga
     8761 tgaatgctgc tttgaactgg cgcgtcagca aaaagcccag cgtcagttga ccgacaaagt
     8821 gaaatcagcg ttacgttatc ccattatcat tttagcgatg gcaatcatgg tggttgtggc
     8881 aatgctgcat tttgtgctgc cggagtttgc tgctatttat aagaccttca acaccccact
     8941 accggtacta acgcagggga tcatgacgct ggcagacttt agtggcgaat ggggctggct
     9001 gctggtgttg ttcggatttc tgctggcgat agccaataag ttgctgatgc gccgcccgac
     9061 ctggcttatc gcgaggcaga aattgctgtt acgcatcccg attatgggtt cactgatgcg
     9121 gggacaaaaa ctcacgcaga tctttacgat tctggcgctg actcaaagtg caggcattac
     9181 ttttttacag ggcgtagaga gcgtcagaga aacaatgcgc tgcccgtact gggtgcaact
     9241 tctgacacaa atccagcacg atatcagtaa cggtcaaccc atctggctgg cgctaaaaaa
     9301 tgccggtgag tttagcccac tctgtttgca attagtgaga acagaagagg catccggctc
     9361 gctggatctc atgttagaca acctcgccca tcatcatcgg gataacacaa tggcgctggc
     9421 ggataacctc gcagccttac tggaaccggc gttgctgatc ataacgggag gaattatcgg
     9481 tacgctggtg gtggcaatgt atctgccaat tttccattta ggcgatgcga tgagtggaat
     9541 gggataacgc tggcgtgttg ctccacgcca gcgttgggag attacaggtt gttgaagatg
     9601 cggttttctt gttccagcac acggataaat gtggtgcgct tggtcagctc tttcaggcgt
     9661 gaagccccaa cgtatgtaca agctgaacgc aggccgccca aaatatcacg cgcggtattt
     9721 tcaaccgggc ctcgcagcgg cagcttaacg gttttacctt ctgctgcgcg atattccgca
     9781 acgccaccaa cgtgacgttt catcgcagac tcggagctca tgccgtagaa cagcataaat
     9841 ttctcgccgt tctcctcaac gatgcgaccg ccgctctctt cgtggcccgc cagcatgccg
     9901 ccaagcatga cgaaatcggc accgccgcca aaggctttcg cgacatcgcc cggcgtggtg
     9961 cagccaccgt cgctgacgat cattccgccc agaccgtgcg cagcatcggc acattcgatc
    10021 accgcagaaa gttgcggata accgacgcct gttttgacgc gagttgtaca aacagaacct
    10081 gggccaatgc caactttaac gatatcagca ccggagagga taagctcctc acacatttca
    10141 ccagtcacta cgttgccagc acaaatggtt ttggtcggcc acgcttcacg cgctttcgca
    10201 acgaactgca cgaagtgttc ggaataacca ttcgccacgt caatacaaac gaagtttaat
    10261 gccgggttca ggtcgagaat ctgtttagtt ttttcgaaat ccgcatcaga cgtgccggta
    10321 gaaaccatca catgtttcag cacatcagcg gaagaattgt tgataaacgc ttgccactct
    10381 tcgaaagaat agtgtttatg cacagcagtc aaaatatcaa aagaagccag cgcagaggcc
    10441 atagaaaatg tgcctacggt gtccatattt gcggcgataa tcggcacgcc ggaccagctc
    10501 tgacctgaat gtttgaaggt gaattgacgt tccagttcaa catcggaacg gcttttaaga
    10561 gtggagcgtt tagggcggat gagaacgtct ttaaaaccta acttcagatc ttcttcaata
    10621 cgcatgtgcg gattcctggg gttaatggct ataagggtaa gagcgcaact ccagtgacgc
    10681 tatcatacgc actaatcaat gcggcgcaag actgcgaaat tgtctttttt tacgctacaa
    10741 tcccataaat ttacctggta aatagtgagc gaacgtttca tcaatttcgc tttcgtgcgc
    10801 tcatttttaa cgttttgata atcatgatga attcccggga atataaatta tgaggtatat
    10861 agttgcctta acgggaggca ttggcagtgg caagagtacc gttgccaatg cgtttgctga
    10921 tctcggaatt aacgtcattg atgccgatat tattgcgcgt caggtggttg aaccaggtgc
    10981 acctgcgcta catgccattg ctgatcactt tggcgctaac atgattgctg ctgatggaac
    11041 attgcagcgc cgggccttgc gcgagcggat cttcgccaac ccggaagaga aaaactggct
    11101 taacgccctg ctgcatccgc tgattcagca agagacgcaa caccagatcc agcaagcaac
    11161 ctccccctat gtactgtggg ttgtgccatt gctggtagaa aactcactgt ataaaaaagc
    11221 gaatcgagtg ctggtggtgg atgtcagccc agaaacgcaa cttaagcgca ccatgcagcg
    11281 cgatgatgta actcgcgagc atgtcgaaca aatccttgct gctcaggcac cgcgcgaagc
    11341 tcgccttgcc gtggcagatg acgtcattga taataacggc gcaccggatg ctatcgcatc
    11401 ggatgttgcc cgcctgcacg cacactattt gcagcttgcg tcgcagtttg tctcacagga
    11461 aaaaccgtaa tgcagaccca ggtccttttt gaacatccac taaatgaaaa aatgcgtaca
    11521 tggctgcgca ttgagttttt aattcaacaa ctcaccgtaa atttgcccgt cgttgactac
    11581 gctggcgctc tgcatttctt ccgcaatgtc agtgaattgc tggatgtttt cgagcgcggc
    11641 gaagtccgca ctgagttgtt gaaagaactt gaccggcagc aacgtaaact ccagacctgg
    11701 attggcgtgc ctggcgtgga ccagagccgt attgaagcat taattcagca gttaaaagcg
    11761 gcggggagcg tattaatttc cgcgccccgt atcgggcaat ttctgcgtga agatcgtttg
    11821 attgccctgg tgcgtcagcg actgagcatc cccggcggct gttgtagctt tgatttacct
    11881 acattgcaca tttggctgca tctaccccag gcgcagcgcg acagccaggt agaaacctgg
    11941 attgccagcc tgaacccgct cacccaggca cttaccatgg tgctggattt aattcgccag
    12001 tcggccccct tccgtaaaca aaccagcctg aatggttttt atcaggataa cggtggcgat
    12061 gccgacttgc tgcgcctgaa tctgtcactc gattcacagc tttatccgca aatttccggt
    12121 cataagagcc gttttgccat tcgttttatg ccgctggaca gtgaaaacgg acaggtaccg
    12181 gaacgtctgg atttcgaact ggcctgttgc taaggagtaa aaatgtcaga aactattacg
    12241 gtgaattgcc caacctgcgg gaaaacggtg gtgtggggtg aaatcagccc gtttcggcca
    12301 ttttgctcca aacgttgtca gctgatcgac ctcggagaat gggctgctga agaaaaacga
    12361 atccccagca gtggcgatct ttccgaaagc gatgactgga gcgaagagcc aaagcagtga
    12421 cattttatct gatgcgacgc tggcctgata agacgcgggc agcgtcgcat caggcatcgt
    12481 gtgcaaatgt cggatgcggc gggaacgcct tatccgacct acaagacctt acatatcaac
    12541 atgttaaaat tgtagatact gataagacgc gccagcgtcg catcgggcat cttgtgcaaa
    12601 tgttggatgc ggcataaacg cctatccgac atacacacgc ttaagcttcg caagtaccgg
    12661 ttcattggct ggcggaaaat catcggcatt cagaccgacc agcgacatcc actcaccggg
    12721 ttgcccttct ttaccccacg gctccccttc ccagctttcg accagccaga accacagtgt
    12781 tatatgcctg tccgggaatt catattccag tatttcaaat agcgaaagat gttgtggggt
    12841 aatcccgact tcttcctgaa gttcacgcac caccgcctgt tccggcgttt cacccatttc
    12901 aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgggt
    12961 tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat
    13021 tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac tgttagcttt
    13081 tattgcaggc ggccatggca ctgcttatat tttttaccag aaccacacgg gcaaggatcg
    13141 ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc agcagagtcg
    13201 tcatcctgat ggctaagctg ctgcatttgc gctaaacgct cggcttccat acgacgctgt
    13261 tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag cgtgctgata
    13321 acttcatatt ttaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat
    13381 tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg cagatagtcc
    13441 atcgcagcca ggtgctcttt ccacagagag tcaagcgttt gcagcatgac gcctttctcg
    13501 aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac ttcgatggac
    13561 tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt atccagccac
    13621 tcggcaattg gcaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata
    13681 tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtcgc tttgaacaca
    13741 tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag ttcgttacgc
    13801 tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta
    13861 cgaatgtcga agttacggct ttcaacttta cgctgggcgt tggcaatcgc tttggtcacc
    13921 cacgggtgct caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact
    13981 cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa
    14041 ccagcatccc cctgacgacc agaacgaccg cgcagctggt tatcgatacg acgggattcg
    14101 tgacgttcag taccgatgat atgcaggcca cctgctgcca gtaccgcatc gtgacgtacc
    14161 tgccagtcgg ctttaatttt ttcaatttgc tctgcagtcg gattttccag cgcggcaact
    14221 tctgcctgcc agctaccacc gagcacaata tcggtaccac gacccgccat gttggtcgcg
    14281 atagtcaccg cagccgggta acctgcctga gcaacaatcg ccgcttcgtt ggcatggaat
    14341 ttggcgttca ggacgttgtg cttaataccg gctttggtca gttcatttga caccagctcc
    14401 gatttttcga tggagattgt acccaccagc accggctggc ctttcgcagt gcgttctttg
    14461 atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaga
    14521 tctttacgaa tcattggacg gttggtcgga acaacgacag tatccagctt atagatggag
    14581 ctgaattcga aagcttcggt atcagcagta ccagtcatcc ccgccagttt ttcatacaga
    14641 cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca
    14701 ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgacggcc ctgcatggta
    14761 cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtcg
    14821 cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg
    14881 gccggagagt acagagactc accttcatcc atgatgcctt ctttaaccag cagttcttca
    14941 atcagaacca gaccacgttc ggtcaggttc acctggcgag atttttcatc caccgagaag
    15001 tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag gtgtggaata
    15061 attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc
    15121 ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt
    15181 ttacgctgta cacgttcttc aggactgaac gccatgttgt cgcgcaggta gtcaaagccg
    15241 tattcgttgt tcgtaccgta agtaatgtca gctgcgtaag cttcacgctt tgccggtgct
    15301 ggcatgcccg gcaggttgat accaactgtc aggccaagaa attcaaacag cggacggttg
    15361 ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg
    15421 gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc
    15481 atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga
    15541 cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc cgggatcaga
    15601 ttttccagca cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt
    15661 tcttcgtcgg agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg
    15721 cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa taatttgatt
    15781 agcataataa aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taacggttta
    15841 ttgttgttag ctgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac
    15901 gggcgtgctg aattttgctt gcggggtaaa atgcgcataa tcaatgcgat aacccttttc
    15961 agacggcgtg ccttcctggg tcaacagcgc gctgagcgta tccagtaatg caagatgttg
    16021 cgcctgaaga ggcaaagatt cttcagcaac gggcagcgtt tgcggtgcca ttgcgaagga
    16081 aagatgacgg attaccgtgc gaatggcatg ttgatgccag taatcaacgg aatagttcga
    16141 attcgggcga cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga
    16201 aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag
    16261 cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc agaagtagcg
    16321 tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta
    16381 ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca gcacgaatga
    16441 agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatcgttct
    16501 tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca gatttgccag
    16561 tcgaatttta tacgacagta taaatgtcgt tatgccagta cggctgaagg cgctttgaag
    16621 gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc
    16681 aggacagcct gcagcagttt gttattcagt gcatgaccag atttataagc ggtaaatgca
    16741 ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc
    16801 acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg
    16861 atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca
    16921 cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag
    16981 ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa
    17041 tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga
    17101 acagtctctt tgatgcgaac aaatttcttg gcgcagttca gctcgtcgat accagcgtca
    17161 agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta
    17221 acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag gtgctctacg
    17281 gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca
    17341 gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc
    17401 ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt atgtaaaccg
    17461 acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc
    17521 caaattacct atccaaccga agtgtactat acattcggcg ggccagttta gcacaaagag
    17581 cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgcagga atgctgggat
    17641 atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacga ctttagcaac
    17701 cggcttctgc tcctgggtca gcggagccat cccgtgctgc tggtagcgat ccatcactgg
    17761 ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc
    17821 tgtcgcaaca acagttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat
    17881 aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc
    17941 atccagacgc aggtcgaagc ccgctgtgat gtttaccagt acgccgcgtg cgccagacag
    18001 gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt ccgcacgatc
    18061 ttcaccgctc gccacaccag aacccatcat tgcgtagccc atttcagaca ttacggtgcg
    18121 tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg cgataccttg
    18181 cacagcgcct ttgagtacgt cgttcgctgc gccaaacgca tccagcaggg agataccgcg
    18241 gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga
    18301 cagttcagtg atcccctgct ccgcgaatgc catacgcttc ttgccttcaa agttgaaggg
    18361 cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg
    18421 agctgcgcct gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc
    18481 ttccagcgcc gcacgcagtg catcgcgatc ctcatcagcc gcattgcggc caacttctgg
    18541 attagcgcca gctcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac
    18601 cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat
    18661 gcgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt
    18721 aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc
    18781 tgtcgcctga gaccgtaatc attgtcggcc tcataaaaat taaaactctt ttcgcagcca
    18841 actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc
    18901 ttcaccgtta agatgtgact ctttcccgta atgaagcaat cccaccgccg tcgaataata
    18961 cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacctg
    19021 cgtatgaaac acgcgctgag cacaggctgc aagaccttca atctgcgctg cgccaccggt
    19081 taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa
    19141 ctgcaatatc tcttcgttga ccaggttgag cagctcggta tagcgcggct cgatcacctc
    19201 tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg gcacttccac
    19261 gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc
    19321 ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc
    19381 ataaggaatt accttagtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt
    19441 accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga
    19501 ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac
    19561 cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca cttttgcctg
    19621 catccgcacg cccgaaagtc ctaccggatt cttgatccct tcctgatagt caatcgcata
    19681 ctcttgcggg atcacatgca gcacacggtg ttcatcgcgc acacgcaccg atttcgcggt
    19741 atggacaacg ttttccacat cttcttgcgt cacttcttct tcagaaatag gcaccatacc
    19801 aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg
    19861 acaatctgcc atcaattctg cctggtcaat ggcgcgctgt acgcacttga ccacggattc
    19921 gaggtcgttc accccgcctt tatccatacc acgcgacggg cagctgccca cgccaatgat
    19981 attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat
    20041 ctccagtcct actaccagtt ttctgtccgt cgccttaatc attgttgttc tgcctgtgcc
    20101 tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca
    20161 gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt
    20221 aaaaccggat aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga
    20281 ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct
    20341 tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac
    20401 acttcattgg cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcga
    20461 tctggcggca cgctgaaagt gtttccttcc gcgtctacca tatgttgatc attccaccgc
    20521 gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg
    20581 ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat gttgacatcc
    20641 tgggtcataa aggtacctgg ctcacccaat gccaggatcg actgccggat atcgtcatta
    20701 cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct
    20761 tccatccagc ccaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg
    20821 aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgctg
    20881 tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac
    20941 caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt
    21001 catacccggt gaggtattgg cttccagcag ataaaactgt ccatcgctgt ccagcataac
    21061 gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg
    21121 caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga
    21181 gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta ttgacggtaa
    21241 aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa
    21301 tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac
    21361 tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc
    21421 cagagcagaa atttctgcta actgcttatc actcaggcct ttttcaaact ctgcgcgggt
    21481 taacgctacc cacggagcga ccggtaaacc ggcaccttgc cacagaagtt tgctgcgtag
    21541 tttatccatt gaaagcgcag atgccatcac tccgctgccg gtataaggca agcccatcag
    21601 ctcgagcatg ccctgcagcg tgccatcttc accgccgcga ccgtgtagcg cgataaacac
    21661 tttctgaaag cccatcgact tcagttgcgt cacgtcgact tctttcgggt cgacaggatt
    21721 cgcgtcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat tcagagaaac
    21781 ttcccgctca gcggaggtcc cacccaacag gaccgcgatt ttatcagtca tgttgttctt
    21841 cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt tttccgatat
    21901 taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggagcc agcatctcgg
    21961 ctacctgcgc cgggtccggc accagaatgg gatcaatttt cccacgtcca cgaattgtgc
    22021 gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga tacacttcca
    22081 gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgcg
    22141 tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc cagcctgcac
    22201 gcgccgcttt aatggtggcg tccacttccg tcgggtggtg gccgtagtca tcgaccagca
    22261 ttgccgtacc gcttttacca ttcacaggct ccagcgggaa ttcaccgagg aaatcaaaac
    22321 ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg tcaattccct
    22381 cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat
    22441 tcagggtgac gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc
    22501 caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg taagtcgtgg
    22561 tctgacgtcc cacacgcggt aataattcgc ggatcaccgg atcatcaaca cacatcaccg
    22621 cacgaccgta aaacggcagg ttgtgcagaa aattaataaa ggtctgtttt aaattctcaa
    22681 agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg
    22741 gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat
    22801 gccccaaacg cgcatgaacg cccgccgctt ttaccagccc accgttaacg aaggttgggt
    22861 cgagtcccgc ttctgcgtag atgctggaaa ccatcgcagt ggttgtcgtt ttgccgtgcg
    22921 ttccggcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac
    22981 ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttatcg gcagaaatcg
    23041 cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa
    23101 tcgtcgcccc aagattcatt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac
    23161 tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccga
    23221 tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg
    23281 ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag
    23341 gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc gctcggtggc
    23401 atccggaatg gatgcggcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca
    23461 cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga taattttggc
    23521 tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa
    23581 cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcaccgtta acgcaccgga
    23641 gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattccgt
    23701 cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc
    23761 tttaccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat
    23821 tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac gaaccggacc
    23881 ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg tacgcaccgg
    23941 gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca ctttggtggc
    24001 aatcttcgcc agccatttat tggttaatcc cgcaataccg ttttgttcat gaagcacaac
    24061 cggaatacct aacgaccacg cggccagacc acctggacct gatacgtagc cgcccatacc
    24121 gagcaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcgttgaa
    24181 gatacgcagc ggggcagcta tcagtgcttt tatacctttt ccacgcagac cagagatacg
    24241 aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcggcagt
    24301 ccccagccag cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg
    24361 gaatacatgt ccaccggttc cgcctgccat caccattaat cgcttccctt gaccactcat
    24421 cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac
    24481 agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact gatcagcggc
    24541 aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg
    24601 aaactaaacc agataccaat agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct
    24661 aatgctttgc ggccaatcga catcgcgcga aaagcgacga agaataccat taaaagtgcc
    24721 agcaccacac cgacataccc cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc
    24781 gcttccggca gatactccag tttttgtacc gagttaccta aaccttgtcc ccacagttca
    24841 ccgcgaccaa acgccatcaa cgattgcgtt aactgatagc cgctgccaaa gggatcttcc
    24901 cacggattcc agaatgcggt aacacggcgg atacggtacg gttcggcgag gatcagcaac
    24961 acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc
    25021 aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt
    25081 gccagcagta acactgccaa caccagaatt acgcccatcg gtttcaggaa gccgcgcagg
    25141 ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca aaacagcgac
    25201 agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc
    25261 cctttaaccg agctacccac taccagaacg atcatcagca ggatgataga tccgagcagc
    25321 atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga
    25381 atgaatgcca ggatcagata gacgccatca cgcttcgcga agaagaacgg atcgttggtt
    25441 aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcgagg
    25501 ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc
    25561 cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccagaat actgaatcct
    25621 ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca
    25681 gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ttgatcaagg ctggcacagg
    25741 ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag cgcatcgcct
    25801 gttccatggt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc tgcgcgccgt
    25861 cccgaccgaa acaatacaga cgtacattat cgccattcag gtaacgcgcc agtgggctaa
    25921 agtccgccga tttaccatcg ccacccagca acaaatgcag cgtgccgtct acatgcaggc
    25981 cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta acccagcgca
    26041 caccgttatg ctccagcaca acttcaaagc gatgcggcag accagtgaat gtggttaacg
    26101 ctttcaggct gctggcacgc ggtaaccctg cggcatctgc cagcgccagc gccgccagcg
    26161 cattggtgta gttatgctgc ccggaaagtt tcatttcttt cacgttcagc actttctcgc
    26221 ctttcacccg cagccaggtt tcaccctgct gatgattcag gtgatagtcg cccatgttga
    26281 cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag gcatcatcag
    26341 cattaaccac gcaaattttt gcgttttcgt aaatgcgcag ttttgctgca cgatactgtt
    26401 gtaaaccaaa cggatagcga tccatatgat cttcagtcac gttcagaatg gtcgctgcta
    26461 ccgcctgtaa actggaggtg gtttcaagct ggaagctcga cagctccagc acgtacagtt
    26521 cgcactcatc atccagcagc atcaacgcag gcaggccaat attgccaccc acaccaacgt
    26581 taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag
    26641 aaccggtgac cgccacaatc ggtgcttgtg cttcacgaca gaacagctcg atatcgccaa
    26701 cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt gcaataccgg
    26761 gactggcgac aatcagatcg gccgccatca gccattcatc attcagaccg cccgtgtggc
    26821 gttctacagc ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc
    26881 gcggcgtcac accgcgagcg aggaaaaagt ccacgcaaga aagcccggtg aggcccaggc
    26941 cgataatgac gacattttta ccctggtaat cagccatgat taacgtacct tcagcgttgc
    27001 cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgtacaatga cgcgcggttc
    27061 cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc
    27121 gcgcagttta aaggagccga cctgcaggat gacggaaagt gtttctacca cgaacacgcc
    27181 ccccataatc accagcagga attcctgacg cagcagtacg gcgataatac ctaacgcacc
    27241 acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg tgttaaacca
    27301 caggaagccc agccctgccc cgactatcgc ggtacagaca atgaccagtt ccccggcgtg
    27361 tcgtagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg cccacgccac
    27421 cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt cgagaccatc
    27481 agtcaggttt accgcgttgc ctgtacccac aatgacgaag taagccagca gaatgtagaa
    27541 cagccccagt tgcggcatca catctttaaa aaatgggacc accagctgcg ttgcgggcgt
    27601 gtctttgcca acaaggtaca gcgcgaaggc gacacccagc gcaatgaccg acatccagaa
    27661 atacttccaa cgagcgatca accctttggt gtctttacgc actactttgc gataatcatc
    27721 aacaaagcca ataacaccgt aacctaccag cactaccaac acgcaccaga cgtacggatt
    27781 ggacgggtaa gcccacagca gtacggagat cacaatcgct gtcaggatca taatcccgcc
    27841 catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcaccacctg
    27901 accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca atgagatgaa
    27961 cagcgcagtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc
    28021 ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa
    28081 gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat cgtaattacc
    28141 tgttgctcag caatcagtga tttaagacgc gcgataagcg cagttttatc ggaaaaatgt
    28201 tcgccaacac cgctggcagt gctgatagca tggctttgtt tgcccacgct taacacgcgg
    28261 tcaataccag ctgcttttgc cgcttcgccc acctgtatat ggcaggcttc gctttcagcg
    28321 cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc
    28381 tggactgctg cggtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg
    28441 ttttccgcca gttggatggg gaacagacgg cctggaacag ctttcagatt tgccagcccc
    28501 gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca
    28561 ttcgcaatat tgtgacgccc cggcaacggc agcagaacat ccacgctacc ggttggggtt
    28621 tgcagggtaa attccgtacc gtgcgaagtc acatggatgt tggtggcggt gaaatcgctg
    28681 ttggcggcat taggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc
    28741 agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag
    28801 atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct
    28861 gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca ggcaatttcg
    28921 ccctgatggt tcgcgccaag ttcaattact gcgtaatcgt actctggcgt taagcgcaac
    28981 agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgcca
    29041 cactggctta aaatcgctgc cgtcatctct ttaacggagg ttttgccgga ggaaccggtc
    29101 agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc
    29161 agacgagtat ccttgacgat tacctgcggc agatcgatat ccagcggacg gttaaccagt
    29221 agtgcacccg caccgccagc tttcgcctgg tcagcaaaat catgggcatc gaaacgttcg
    29281 cctttcaggg caacaaacag gcaacccggc gtcagttttc gtgtgtctgt ggttacagca
    29341 tcaagggtga tatctgcccc ttttagttca ccgttgagaa tatcggtaag ttggctaagg
    29401 gttacgctaa tcatgcaatc acccccagca gacgcgccac cgtgacgcga tcggagtagt
    29461 ccagacgctg attgccaaca atctggtaat cctcatgacc tttgcccgcg accagtacca
    29521 catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg ccttccatca
    29581 ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggtt
    29641 cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcgaactct tcagcaattg
    29701 cgcccatcag tggacgtttg cctttatcac gatcgccacc acagccaaag acgcaccaca
    29761 gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca tccggtgtat
    29821 gcgcgtaatc caccactacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccac
    29881 aaaccggctg caggcgtgcg gcagttttca gcaggtcagc caacggatag cccagcgcca
    29941 gcagcgtcgc cagcgccagc agcaagttgc tgacgttaaa agcgcccatc agatggcttt
    30001 caatttcgcc atcgccccag cttgagctaa agcgaatcgt cgcaccgctg tcgtgatagt
    30061 tcacttctgt cgctttcaac cagcgtccgt gacagttcgg attgatatga tcttccattg
    30121 ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca tcgtcggcgt
    30181 taataatcgc ctgaccgcaa tgatgctcag aataaagcag ccatttcgcg gcttcgtagt
    30241 gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag accgacgccg
    30301 caaatttcaa tgccgccaca cggtgctgta ccagtccgtg ggaggaaact tccattgcgc
    30361 aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatcg actgccgaac
    30421 cggttgtatt ttctgtcggg atcactttcc ccagcaggcc gttaccaacg gtgcccatta
    30481 ccgcgctggt ttcgccaagc agttggctcc actgcgccag cagctgggtc gtcgtggttt
    30541 tgccgttggt gcccgttacg cccacgagac gtaaattgtc agagggttca tggtaaaagc
    30601 ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc ggtacgccgt
    30661 gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata atggcagcca
    30721 caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag
    30781 ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc
    30841 gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct
    30901 gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggt
    30961 tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg cgcggaaacg
    31021 gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc
    31081 ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat gtagcgaccg
    31141 tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc tttaatcgcc
    31201 gccttcacgc cgccgccgcc cggtagcgcc acgctttcca tcatatgcac cacggtgcga
    31261 acaatggatt ccgggaagac tcgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac
    31321 agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacggt
    31381 gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt
    31441 ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt
    31501 ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgctaacgc cagcttggaa
    31561 acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct gtagcgtgcc
    31621 acgtctttga tttcgtggcc gttaattcga taaggaacgg tattcagtac cgagttttcc
    31681 cgcactacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt tgaacccggt
    31741 tcaaacacgt cagtgatggt acggttacgc atcgcctctt tcggcgtgcc actcagattg
    31801 tttgggttgt atgacgggct gttagccatc gccagcactt caccggtgtt gacatccacc
    31861 agcacggcgc taccagattc agctttgtta aaggccaccg cgttgttcag ttcgcgataa
    31921 accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgccgc ctgactgtcg
    31981 gtggaagaga tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc
    32041 ggctgcccgg taagccattt atcgaaactc ttctcgacgc cctcaatccc ctgactatcg
    32101 acgttagtaa agccgatgag gtgagcggtc acttcgccgg atggataata acggcgagat
    32161 tcttcacgca gatgaatccc cggcagtttc agttttttga tgtactctgc catgtcaggg
    32221 tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat gcgggctgaa
    32281 agctgatcca gcggaatatt gagtgcgtta gccagcgcct tccagcgatc accgacgctg
    32341 ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc
    32401 accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga
    32461 acgcgaagag aacgcatgtc gccctctttt accagcatat ccggggagat aacttgtaac
    32521 cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca
    32581 aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc
    32641 gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga
    32701 tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg
    32761 agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc
    32821 accagttgtt cgcgctgtgc ggtcagtaaa cgggtatggt gcgccgtggt taccacggtc
    32881 accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga
    32941 tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga tcctttaact
    33001 ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca atacgcagaa
    33061 ctgaactacg ggcacgaggg ttctcagcca cctcttcttc acccggcatt aacttgccta
    33121 gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg
    33181 ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca
    33241 gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt
    33301 ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg
    33361 tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc acgacttccg
    33421 ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gcgcgggcaa
    33481 tgcgtttggc aaaacgctct tcaccatagg ttttcaatac ccaggcgata tcggcttctt
    33541 ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca
    33601 gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc ggtgaagaga
    33661 cgccaagatc gaggagaatg ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt
    33721 cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg
    33781 caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct
    33841 gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat
    33901 caggacggat attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt
    33961 tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc agtagccaac
    34021 tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca
    34081 aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt
    34141 actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac
    34201 agtaggcgct gcacacggcg ctcaaccggg ttcatgctcg acagacgcga taatttttgc
    34261 tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg
    34321 gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg ggtaggcact
    34381 gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca
    34441 ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa
    34501 aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat
    34561 atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg aatagctgta
    34621 agaaaaaatc cctcatcgtc atcaccgttt taaaatgact cactattttc cacagcaaat
    34681 ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt
    34741 ccctgttgcg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg
    34801 tttgttaaat cggcaattca tctctgcatg taacaaaata atgcaaaaac gcgctatcga
    34861 tatcacttaa ttattgtgaa tttcagctgg tgaggtaatt aacgggcagg tttaaaagca
    34921 tttatcaata atagccagga atagtcttat ttactttaag gatattgatg tccattcccg
    34981 tactcaacgc gccagagaga aattcacctg gcgcgtattt ttgttcgcgg cttagctacg
    35041 gctgagcacg ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt
    35101 acgcggttcg tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg
    35161 acgttgagcc actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa
    35221 ggtggcaatt gccaggtcag aaggcagttt gccgtcacga cgtagcgtga catccatcac
    35281 cccttgcaac aacgcaaacg acgttgtgaa cagtgcctgc ggcatcggat gcgtttccag
    35341 ccatttttcg aataactggg cagccgcctc ccgctcatag ctgttggcat aaaggaaatg
    35401 cacttcgcga ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac
    35461 agaaagctcc ggcagcgcgc caagataaag taccgtctcg gcgggaaact tacgtaactc
    35521 ttccgccagc atttcggcat catcctgatc ggcaccaacc acgctggtga agtgttcacg
    35581 atcgagggcg cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa
    35641 aggatgctca ggtggcaacg acgtcgaaac aataatggca tcaacctgac gctgtaaaag
    35701 atgctcgata caacgcattt cgttgtcggg ctgatcttcc gagcaggcaa tcagcagttg
    35761 ataacccctt tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt
    35821 ctccagatcg gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc
    35881 cacggcgttc gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt
    35941 gtcgctcaca cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga
    36001 cactcccgcc agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa
    36061 aaaccatagc cattgtactg gtatgggtta aattcattaa catctaagcg cagaaaatta
    36121 actgcggcaa ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga
    36181 catgttgggc tgcaaattgc gcattgagat cattctcaac gcattatttt atcgccgcgc
    36241 gaaagtccga ctacaccaga gcgagcaact tccacaattt tcgccacatc gcgaatcgat
    36301 gctaaaaatg catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggt
    36361 gtgacatcga taatttgccc acggaatatt tccgtattac gtttcacttc atcacgcccg
    36421 taaccgctgg cctgaatttt caccagcatg atttcccgct caacgtgcgc gccctgcccc
    36481 aactcgctca cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat ttgctcaagc
    36541 actttttcat cgcccacggt ctggatggtc atacgcgata atgttggatc gtcggttggc
    36601 gcaacggtca ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat
    36661 aacgcgcctg attcattttc gagtaagact gataatatcc ggcgcataat caggttctct
    36721 ccgttttgct taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt
    36781 gctcactgcc atcgacggtg acatcaacaa acaccaggcg attattgcgc acctgttcca
    36841 gcgcctcgct aagtttgctt tccagttctt gcggatgaga gatctggatc ccgacgtgac
    36901 cataggcttc cgccagacga acgaaatcgg gtagtgactg catataagat tgtgaatgac
    36961 ggccggaata gatcatgtcc tgccactgtt tcaccatccc cagatagcgg ttattgagat
    37021 tcaccaccag taccggcaac tcgtattgca acgctgtaga cagttcctgg atgttcatct
    37081 gaatactgcc gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc attttgacgc
    37141 ccagtgccgc aggtaaacca aaacccatcg tgccgaggcc gccagagtta atccagcgac
    37201 gcggtttgtc gaatggataa taaagcgcag caaacatctg gtgctgcccg acatcggacg
    37261 tcacgtaagc gtccccatta gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa
    37321 tcttttcact gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatctgct
    37381 gccaccagtc gcggatctca tccagcggtt gatggacgga ttcttgcgac aggagttcaa
    37441 gcatttgttc gaggacctgg cgagcatccc ccacaatcgg gatatcagca gtcacggttt
    37501 tagaaatgga agtaggatca atatcgatat gcagaaccgt ggcatttggg cagtacttcg
    37561 ccagattgtt cgtcgttcgg tcatcaaaac gtaccccgac ggcaaaaatc acatccgcgt
    37621 tatgcatcgt catattggct tcgtaggtac cgtgcattcc caacatgccc agtgcctgac
    37681 gatgcgttgc cggaaacgcc cccagcccca ttaatgagga aacaacgggc agattcaacg
    37741 cctccaccgt ttcttttagc tgctgatgac agcccgccgt gattgcccca ccgcctacat
    37801 agacaaccgg tttttttgcc gccaccagcg tttgcagagc gcgcttaatt tgccctttat
    37861 gcccggaggt cgtgggattg taagaacgca tactgaccga ctccggccag acatagggta
    37921 atttgttcgc cggatttaga atatctttcg gtaaatcaac gaccacgggt ccaggacgac
    37981 cacttgccgc cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa
    38041 ccagaaaact gtgtttaacc accgggcgag aaatccccac catgtcgcac tcctgaaagg
    38101 catcgtagcc tatcaacgag gtcgctacct gcccggaaag gacaactaag ggaatggaat
    38161 ccatataagc ggtggcaatg ccagtaatcg cattggtcgc ccctggaccc gacgttacca
    38221 gcacgacgcc gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc accgccgcct
    38281 gctcatgacg aactaaaaca tgatcgatac cacccacggt atgcaatgca tcgtaaatat
    38341 caaggaccgc gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc
    38401 ggacgaccat ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa
    38461 aatgtgtaaa acacattaac cgtccgatct tgcttaagca attggcagcc tgagacataa
    38521 acacggggta ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt
    38581 acagaggatt aaattgataa taaatcggct gaatcccaca acttacagca atttctctat
    38641 ttttttaatt aatcctaaaa tagcgagatc acacattcag aataaaattc tatattcaat
    38701 aaaaaatgca gaataaacca gacattaatg tctgacctat tctgcattca gttagcgttt
    38761 gcaaattgag actaattgct cttccatcca ctgatggcct ttatcgcgcc cggcagcttc
    38821 atgccaggag agataacagg ttctgctgtt ttgttttaac ggcagcggta atacctgtaa
    38881 ctctaaggat tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccagatgcgt
    38941 ttgcgacacc acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg
    39001 cttatctacc gtgtcatacc aggtttgact aaatgacgcg aaacgatcga gcgaaaccgc
    39061 agcatgttgt tcgttataaa catcatgttt cagtaacggg cctttaattg ttggatgatt
    39121 tttgctggct accaacacca tttcatcttt aaataatggc acactggtaa attcagggcg
    39181 atggaagtct tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt
    39241 gttctgattt aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg
    39301 attataaatc tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac
    39361 acgttcactg ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa
    39421 tgcctgacga actgagccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc
    39481 ataacgaaca aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac
    39541 cgcaggttgc gacattccca gaacatgagc ggcacgggtg atgttttgct cctgcatcac
    39601 ggcatcgaaa acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc
    39661 cgccgtctct ggatgatctg tttgaacctc tggcatactt aactccactg tcacacttaa
    39721 ctccctttcc cttattggaa tgcagagaaa taatcctcga gaatatgatt tatcatgcat
    39781 ataaaataag aaaaaggaaa atgagtaaaa ttaggaaaac ataaagatac gggttctgat
    39841 aaaaacagaa tccattgccg tggaaagcgt catttaccac agcataataa tccataatgt
    39901 ttttgttgcg aaaacaatct aatcataact accacgaata ctcaatcatc cacaaaatgg
    39961 attaaatgtg ct
//
LOCUS       DAZGTJ010000036        39341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000036 DAZGTJ010000000
VERSION     DAZGTJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.036"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(123..746)
                     /locus_tag="ACHK8E_002360"
     CDS             complement(123..746)
                     /locus_tag="ACHK8E_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0344619.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(835..1347)
                     /locus_tag="ACHK8E_002361"
     CDS             complement(835..1347)
                     /locus_tag="ACHK8E_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344620.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            complement(1629..1865)
                     /locus_tag="ACHK8E_002362"
     CDS             complement(1629..1865)
                     /locus_tag="ACHK8E_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0344621.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(1934..2509)
                     /locus_tag="ACHK8E_002363"
     CDS             complement(1934..2509)
                     /locus_tag="ACHK8E_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344622.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            complement(2767..3333)
                     /locus_tag="ACHK8E_002364"
     CDS             complement(2767..3333)
                     /locus_tag="ACHK8E_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0344623.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            complement(4913..6517)
                     /locus_tag="ACHK8E_002365"
     CDS             complement(4913..6517)
                     /locus_tag="ACHK8E_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0344624.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(6947..7852)
                     /locus_tag="ACHK8E_002366"
     CDS             complement(6947..7852)
                     /locus_tag="ACHK8E_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0344625.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(8306..8758)
                     /locus_tag="ACHK8E_002367"
     CDS             complement(8306..8758)
                     /locus_tag="ACHK8E_002367"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0344626.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(9272..10003)
                     /locus_tag="ACHK8E_002368"
     CDS             complement(9272..10003)
                     /locus_tag="ACHK8E_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0344627.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            10674..11204
                     /locus_tag="ACHK8E_002369"
     CDS             10674..11204
                     /locus_tag="ACHK8E_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0344628.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            12884..13687
                     /locus_tag="ACHK8E_002370"
     CDS             12884..13687
                     /locus_tag="ACHK8E_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344629.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
     gene            complement(13744..14703)
                     /locus_tag="ACHK8E_002371"
     CDS             complement(13744..14703)
                     /locus_tag="ACHK8E_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344630.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPNTINFSESIRINYSYTLVPAYIRKKVSSLMGDSELRY
                     VYSFPGSLYSYLLEKHPLSEVKKLMEDLPSTEDENFRRSETGSQYLKGILGVKTRVSE
                     LFIRQFSLPFRFPHTKEYVRDVIFKPVENDMSLSEELAHVLFFTLLHSDIMSRFSLRE
                     LFFRIESGRDNNKKRDLIREYVSDVTKEYSLCIPGIVSRHDKK"
     gene            14817..15701
                     /locus_tag="ACHK8E_002372"
     CDS             14817..15701
                     /locus_tag="ACHK8E_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0344631.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            15820..16497
                     /locus_tag="ACHK8E_002373"
     CDS             15820..16497
                     /locus_tag="ACHK8E_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344632.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            16503..16741
                     /locus_tag="ACHK8E_002374"
                     /pseudo
     CDS             16503..16741
                     /locus_tag="ACHK8E_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            16830..17648
                     /locus_tag="ACHK8E_002375"
     CDS             16830..17648
                     /locus_tag="ACHK8E_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0344633.1"
                     /translation="MRLASRFGYANQIRRDRPLAREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGNEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            17987..18460
                     /locus_tag="ACHK8E_002376"
     CDS             17987..18460
                     /locus_tag="ACHK8E_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0344634.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            18476..18952
                     /locus_tag="ACHK8E_002377"
     CDS             18476..18952
                     /locus_tag="ACHK8E_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0344635.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            19021..19242
                     /locus_tag="ACHK8E_002378"
     CDS             19021..19242
                     /locus_tag="ACHK8E_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0344636.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            19405..19773
                     /locus_tag="ACHK8E_002379"
     CDS             19405..19773
                     /locus_tag="ACHK8E_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0344637.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            19863..20240
                     /locus_tag="ACHK8E_002380"
     CDS             19863..20240
                     /locus_tag="ACHK8E_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0344638.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            20237..20725
                     /locus_tag="ACHK8E_002381"
     CDS             20237..20725
                     /locus_tag="ACHK8E_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0344639.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            20745..20942
                     /locus_tag="ACHK8E_002382"
     CDS             20745..20942
                     /locus_tag="ACHK8E_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0344640.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            21027..21893
                     /locus_tag="ACHK8E_002383"
     CDS             21027..21893
                     /locus_tag="ACHK8E_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0344641.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            21965..22228
                     /locus_tag="ACHK8E_002384"
     CDS             21965..22228
                     /locus_tag="ACHK8E_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0344642.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            22225..22551
                     /locus_tag="ACHK8E_002385"
     CDS             22225..22551
                     /locus_tag="ACHK8E_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0344643.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(22965..23501)
                     /gene="yghD"
                     /locus_tag="ACHK8E_002386"
     CDS             complement(22965..23501)
                     /gene="yghD"
                     /locus_tag="ACHK8E_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFP0344644.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(23503..24681)
                     /gene="gspL"
                     /locus_tag="ACHK8E_002387"
     CDS             complement(23503..24681)
                     /gene="gspL"
                     /locus_tag="ACHK8E_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP0344645.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(24678..25655)
                     /gene="gspK"
                     /locus_tag="ACHK8E_002388"
     CDS             complement(24678..25655)
                     /gene="gspK"
                     /locus_tag="ACHK8E_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP0344646.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(25652..26257)
                     /gene="gspJ"
                     /locus_tag="ACHK8E_002389"
     CDS             complement(25652..26257)
                     /gene="gspJ"
                     /locus_tag="ACHK8E_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP0344647.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(26254..26625)
                     /gene="gspI"
                     /locus_tag="ACHK8E_002390"
     CDS             complement(26254..26625)
                     /gene="gspI"
                     /locus_tag="ACHK8E_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP0344648.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(26622..27185)
                     /gene="gspH"
                     /locus_tag="ACHK8E_002391"
     CDS             complement(26622..27185)
                     /gene="gspH"
                     /locus_tag="ACHK8E_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP0344649.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(27189..27644)
                     /gene="gspG"
                     /locus_tag="ACHK8E_002392"
     CDS             complement(27189..27644)
                     /gene="gspG"
                     /locus_tag="ACHK8E_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP0344650.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(27661..28884)
                     /gene="gspF"
                     /locus_tag="ACHK8E_002393"
     CDS             complement(27661..28884)
                     /gene="gspF"
                     /locus_tag="ACHK8E_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP0344651.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(28884..30377)
                     /gene="gspE"
                     /locus_tag="ACHK8E_002394"
     CDS             complement(28884..30377)
                     /gene="gspE"
                     /locus_tag="ACHK8E_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP0344652.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(30377..32437)
                     /gene="gspD"
                     /locus_tag="ACHK8E_002395"
     CDS             complement(30377..32437)
                     /gene="gspD"
                     /locus_tag="ACHK8E_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP0344653.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(32467..33426)
                     /gene="gspC"
                     /locus_tag="ACHK8E_002396"
     CDS             complement(32467..33426)
                     /gene="gspC"
                     /locus_tag="ACHK8E_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP0344654.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(33444..33854)
                     /gene="gspS2"
                     /locus_tag="ACHK8E_002397"
     CDS             complement(33444..33854)
                     /gene="gspS2"
                     /locus_tag="ACHK8E_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP0344655.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(33920..34729)
                     /gene="pppA"
                     /locus_tag="ACHK8E_002398"
     CDS             complement(33920..34729)
                     /gene="pppA"
                     /locus_tag="ACHK8E_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFP0344656.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(34859..39341)
                     /gene="sslE"
                     /locus_tag="ACHK8E_002399"
                     /pseudo
     CDS             complement(34859..39341)
                     /gene="sslE"
                     /locus_tag="ACHK8E_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT        10051 a        10256 c         9209 g         9825 t
ORIGIN      
        1 gataatatcg taatcggacc ttgctattcc cgtgtcctta gcacatgttt tcaggcacgg
       61 ggataaagta taaagaatgt cgtactgctg ctgccagagc aatattcccc ctgatggtcg
      121 tatcagagat ggtatgccgg tattttgcgg gttgttcccg tcaggttatc gctcacttcc
      181 acggacgttg tcaccaccgg cattccgctc tgtcttagtc tcaggacatc agccgcaata
      241 cgctgactgc cagaccagaa aaggccgtcc agtgcggtca ccagtaagcc cgcctccagc
      301 gcatacttca gccgttcacg gggcgctttc acttccctgg caacctgctg atatggcgat
      361 gatgtgcttt catttccaac cacgcgacga atacggtgag acagatgata ccggtatgta
      421 tccggcacac cggaaaggct ggccttcagg ctgtacacgc agccaaagcg tttatcgttg
      481 aacatcacat ttttctggct gatgtcccat tcttcacgca gcgcggcaat cagttgtggt
      541 gtacggataa gcaacaagcg aaaaggcagt tcaaaactgg tgacgtaatc cacattcagc
      601 agagcaatac gcagccgttc ttctggcccg gcctctgtct gccggcactc ctccagaaca
      661 tcctgccact gccagcgaag acggggggat tcattcagct ctgtaaaact gtatttattc
      721 gccagataat caattcgtgt acgcatactg aaaagaattc tgtataaaaa ttcatctgac
      781 aaaactttat cagtccataa gaatcaacgg aagcgcagct ggttcacacg accatcacca
      841 gttgattgtt gtcataaccg gtaacacaaa ggattgaacg actgttttta acgcaacata
      901 ctggccagat gtatcacccc ggacgacctg aaaacgcttt tccacccggc attgtccgcc
      961 agcgcttatc accttccagg tctgttgtag taaatccacc agttgtgaac attcttcatc
     1021 aactgtgaca ctggctcgcg ggtgacaaat actcgtctgg ctgagcagca acaggcatcg
     1081 aattgttgct cctctatgtt gttcccgcaa ccagcgtaat accaccggcg aggatggact
     1141 ggccgtgtga ttacgctcag taatacgttc gtgcacctgt cggtaaaagg aagtacagaa
     1201 tgcatgaatc tgttgcccgt tgatgtttcc ttctgccgaa tagagcgtga agtgaaatgc
     1261 caacagattt ggccagtggt ccaccgcctg ctgaacaaaa cgccggatgt ctcccggctc
     1321 tgaaattaag gctttggtta tttgcacgaa atgttccttt tcaaaccctg ttgaattttg
     1381 ttaagccggt gcagacacac ggaaagcgca catcaacacc ggcacgatac gatacaaaca
     1441 ccagtgactg gcgggacgaa gggaatgacg aatcagtcag gaaatgatga caaacagttg
     1501 gctgaccaga ggagtcagca gaaagatgag cgggagttgt cagcgccagc accaggagca
     1561 gggctgatct ccacaatacc agcgccgaca ttttgcacat tgccagcgta ccggcatcac
     1621 aacacgtttc atgcagctgc tcctcgtgat tcctcaattc gctgctgcat ccactgctcc
     1681 acttcacttt tgtaccaacg gctgctgcga cccagtttta tgggtttagg aaaatgacct
     1741 tcactgatca gcttgtaaaa ccatttatcg gtcataccgg tgaaactggt gatgtagttc
     1801 atgtcgatca ggggatcgtc ggcacgcagc aatatactgt cgtggcctgt tattgaggtc
     1861 aacatagtca ggatgtcctc tgtgagattt atggaggttg ggcaaatgct ggtgaatctg
     1921 ctgaactgag ctttcagaac agtcctgcag atccgggata gccgggatta ccggcgaggc
     1981 aacgggcttc cggatcgcac acaccacgac gatcgcgggc gaaatcactg aacgcccaga
     2041 tcaagtcttt gaggatgtct tcgcagtccg gtgaacaact gtccagcagt acatccctgg
     2101 tgatctgctt ctggtacggt gctgcttcac ccggtgacgc gaagcccagt acgttgctcc
     2161 aggcatccag aattagccga tccagttcag ggccgtcgaa atactcctgt ctgacacaca
     2221 gcaccacgtg gtattccggc ttttcaggat gacgggtcag gagccagcaa aggtcgcgtc
     2281 cgggtttcgc attcatcagc gcactgacgg tttcgataaa atccatacca aaccaggtcg
     2341 ttctgctgtt gaaggtattg tcatattcac acggcagacg aagcaggatc cgccgtatcc
     2401 gtgagccgga ctgctcacgc agcgcacgct caagaacgcc gatgactttg ctctgataag
     2461 atggccagaa gggtgccagg ctatagtcga aataagggat agcattcata agatatgctc
     2521 cattatgcgc agcccacaat gcgtcctgcc cgtgaataag ctgacgtgtt atggtttgcg
     2581 gatggggttg atggaataag tgaaaaaccg gggcaaaacg ggaagatcag gtaactgaca
     2641 aggaacaaaa caccggatgt ggttcagggt gaatgatctc cattgacttc ggttcacatc
     2701 attaagcagg gcggtaagtt tttactaacg gagtgtttcc gggccaggaa actcaaggtt
     2761 taagcttcac ttctgaagat tacgtggacg ccctgcggca ggacgctcag gaacttcact
     2821 gcagccgtag gcacacagcc cgtctttttg ttcttctttc gccagataat gcagggcacc
     2881 gcgaatatta tcgatgcttt caggatcgtt gtggcgcaca ggaatgttga tgttgtatgt
     2941 ataatgcggc tgatatgtac agcggtgtgc cgaaccgctg ttatgcgtaa tagaccgcca
     3001 gcattcagct atccgctccg caaagggata tattttttta accctctgac gatcgatcca
     3061 gaaaaccgca tgtgcatgaa aaccatgatc agccgtccat tcaatcaccc agaagaagcc
     3121 aacgatattt tcaaattgtt ccacctgctc cagcagcata cgcaactgca attcaagcca
     3181 gcgatgatca ggttgtaaga aatcaggcgt atctttcctg tagaagaaat ccatccgtaa
     3241 agctcgcagg caggaataac gtgccagcat ttgattgatg tgattgtcaa gtaaagcggt
     3301 aaaaaaccag tttggtgtgt aagatttagg catgttgatg actcctgaaa gttaatgatg
     3361 ttttaagctc tgtgaacgaa ttaatgcttt cgctcataat cagatctaaa acggtaataa
     3421 cttacagatg gtatttatca ggcatgctgt agcagaatgg atacgacggg ttattaacga
     3481 tacgcagcat tatgcagaaa gaaatggatt atctgatggt attagctaaa gagatgagat
     3541 aaacgaggag cgaataaaat acagaatcaa taaacataca tcacgaaata ctgcaaaaca
     3601 cgcgataaaa acccacgacc aatagttcaa aaaacatatt acgaccaata gttcaaaaaa
     3661 catattacga ccaataacat cataaactac aaacttgctc tcagtgagtc tgaaaaaacg
     3721 tgtacaaggc caacatatac ctgtaaccca ataaacttac acgcactcat ttaatcggtt
     3781 aaaaacaact catgttcaag atatgaacgt taacgggtac acggtaccag aataaaatat
     3841 accattgatg gataattttc attttgttta acatacaaga taatcagaaa cgaacaaata
     3901 taatcaggaa ttcactctgt taatttcatt gtttatcctg cgctgttaaa catgacggaa
     3961 tcatggatac cgtatcaaag cgtaagcatt cagttaatca gtgatgacag agaattgatg
     4021 ttcacaagaa cagttaaaac acttcagata aacaatcatc gttaagtcaa tacagttcat
     4081 gatagataag attaaaatca attactgttg cagtgaagac tgataacaga tggatatcag
     4141 caacagcgta gtagagaata acttatctga gtatcatatg agttacagta attatgattg
     4201 agggatggag taaatacgat aacaagagta atactaatgg aatgtgtaaa gattgagagt
     4261 gagtgaagac aggaaataaa taacaataga gattgtatga aaaagggagt gagtataata
     4321 atagtaataa acctaaatta aaattatcct tgtttgatga gtctaaatga ggaattatcc
     4381 attctggata tgagtctgac aaaacaattc tcttacagaa aaatatcaaa tcgtttttct
     4441 tcaatcccct attgaaagct aaacaacatc tcttatcagt aacagactga taacctttcg
     4501 ttattcagaa aaagatcgac atcgtgatat acgccagcac tcatcacgac aacaatcaaa
     4561 caaagttata tataaaaact cacataatat ggttaataat tagcttcaat ccatgtgaaa
     4621 aataacattt cgattcactt tcgagatatc aaaaacgaaa acaattcttc caccagtgca
     4681 cacattcctg cattcaacca gctctgacaa caggtcatta tgaaaacaaa atcaaatcac
     4741 atacatggta catgtactgc taccgttgct accgtagcga ttttttcaga gaaattttcg
     4801 ttcacacccc ataattggtg aatatttgca caaatgcagg atatgaaaaa cgccttacca
     4861 tcaaccttcg aatgaaggct tatgtgtttt gtttgcttat tgtaaagctg tttcaaaata
     4921 aggtataggc tgaaaaaggt aacgcaaatg acggaattaa tgccagaggg atattaacgt
     4981 gatatgtttt cgcattatga ctattcaagg gtggaacaat atcaaactgt tcgtaagaca
     5041 ttgcaccgca aggaaggagg taacgattgt tacaatgaac agaaacataa agtgcctgat
     5101 gcgatcgcat tttgatgata cataaataat tctgtgcgat taactgattg agtacaggtg
     5161 taagtttttc tgctcttctc agacgattgg ggccatatct ctcaatgtct gttttcctga
     5221 tagcatacaa attattctgc ttgattcggg tcataatcca tgtatacagt tcacgggcat
     5281 cctgttcaaa ctggcatcgt tcagacatag gataaaatat tgtgcttgcc tcctccagat
     5341 accactccat gagatgcatc gctttccaaa gcgctattga tgtaatttcc tcatcgtctt
     5401 tatagcaata gcaggtgaat aaacccgcta tcctcagtat attagaacct gctttcagaa
     5461 caatatctct gatgtgttcc cattccccac ctgcaagcgc tttgctttca attttaatct
     5521 ggttttcctg ccactctccc aacgctttct tcgaaagttt taatgtcttc ctttcgcttc
     5581 tcattccggg ggaattaatg ctatggcttg acagaaaccc gttaatcttt ttatgaaact
     5641 ccaccagggt tggtattgtt acgaactcgc ctttcctata atcacgtacc cgggaagagc
     5701 ctccaacctg actgaaaaga aaacgggaaa gaaatccgct tccccatgcc agtacatcat
     5761 ttttatttat ataatccgta aaaacattcg gctgagacat taatgaaaat gtcaggcgtg
     5821 gagtaatatg atatttttcg tctgctctcc caaaatcaaa cggttgtcca ctccatgctt
     5881 tattcaacag acccggatta tttttcagat agcttctgaa aaaagttacc gcttcatcag
     5941 aaataatccc tgcctccgga tgttcgttga gtccttccgc aagcgcttta agcgaaacat
     6001 cttcatagat aaaattcggt cgaaccggac gtgtcggttt atttctttcg tgattacgca
     6061 gtgcctcttc ttcctgttct cccggatccc cccggttaac agcctttctt aaattggcag
     6121 caagagcttt ctgccgggta ttccagatct gtagttcagt cttataatct ttgtttctct
     6181 cttcgtattg ttgaatgagt cgatcggcaa attcgtaaca gggggtcatc accagtctgt
     6241 ttatcgttgt ttttcccgcg cctggctctg cgattgccaa cagataaagt gagcagggtt
     6301 ccggcatgtt agtgtgagga tgaacaacgt caaccaatga ctgacaggag agtgagagcg
     6361 ttgccagtac agtattaaaa atgagctcca gaggaagttg ggtacttgaa tacaagtcgt
     6421 caatgagata cgcaagatca ctaggaaata atctgtaggt tacatctgtt gggaagtggg
     6481 gggatgtctg caaggcttgc acataatcgg gattcattgt aatggacctc cttcacatac
     6541 tgtaatcagc acaaagctta ttgttcagtt cagaaacata ccaacattgt gagtatgttc
     6601 gctgacgcca ataataaaat gactgatctc cattcaccag tcccggcgca tttatggttc
     6661 tgacaaaagt aaacaacatg ggagccagaa ctctgcatcg cagcgacaaa ctatggatgc
     6721 atgaaaaaca gggaacatta cgcaccagag cgttgagagt cttcctcctt gcccaatacg
     6781 aaagtcatct atccacccgt cttcaatctt cgggatggtt gttttatgaa cagagtaaaa
     6841 gtttgaagca agctgagaac ctctgtttcc acgtaaaggt ccgctatgaa gaaaaatggg
     6901 agtttgcatg ctcaactaat ttacctgtag gtgcttgtta ctgtagttat tcaacaatcc
     6961 cacaaatatc ctgcatcaga ttacgggctg tctggctaat gccgccaagc tggaaaaaac
     7021 cgtgaatgac accgaggtaa cgctgggcgg tacactttac tccttgttga atcatacggg
     7081 aaaacagttc ctcaccttca tcgcgtaatg ggtcatattc cgctgtgatg atatgaacag
     7141 gtggtaaccc attgaaatct tttctgaata atggactggc ttccggatgt tctaaatcca
     7201 gaccggaaaa ataagcctca taaccactaa gaaacgtgtc ttctgtcagc acataatcac
     7261 ttccgtttaa tttgtaacta tcgcatgatg ctgttgcatc cagcataggg taaataagta
     7321 ttaattttcg tggtaaccac tgagcgatgt ttttcacccg cagtgctgtt atcagtgcaa
     7381 gatgtccccc tgcgctatct cccattaacg ttattttgtt gcgatcaact ttcaacattt
     7441 ctgcgtgttg ccatacaata ttagcggcat gttcagcatc gtcatgtgcc gcaggataaa
     7501 tatgttccgg cgctaatcga tattgtacag ctatgacacg acagccgctc aggaaagcaa
     7561 gctgtcgaaa ctgattatca tgggttacaa aaccaccact gacaaaacag ccaccatgat
     7621 aatagacaat tgcggatgac aatttatcat cgtttaaagg cgaaaagata cgcaataaaa
     7681 tatcatcaat aatcacatca ctgatgttta cgcgggtttc tgtatcgccg gccagagcag
     7741 tacttgcaat atatcccgcc cgacgttcat caaatgtctg atcacgacag gatggacgac
     7801 cacccgcaac aaaatcattt acaaggtttg cgatttcctg ttcaagtgcc atacaaatca
     7861 actccgaccg ataatattta aacaacgcta cgggaagtat aaatactctg accaaaatat
     7921 aacaggtgaa tatccatagc ttcaacaggc gtaatcaata agggcgtacg gagcttgccc
     7981 gttattgctg caggatcgac agactggttt tcgtccttgc gcatgtaaca gtacaaccac
     8041 agagaaaagc atcaatggac actttatgtt gcgtcagggc agcgaacgac tccttttgtt
     8101 gacgacacat atcaccttct gtactggcaa ttacggcatt cagagtctcc ctgagtttta
     8161 agctcctgct cttacggacc agcgacaagc aataaataac tatgacaatg aatctaataa
     8221 cagcataaat atgatactca tcgtgattag tataatcggg gcagaattat attcactgtc
     8281 tgatttatta ttgggttaaa tataattaaa gtgttctcaa aagtctggct ggattaccgg
     8341 catacacgcc ttttaccagc acgggctttg taaccacact tccagcacca atcactgctc
     8401 cactgcaaat ttttgttgtt aaaatagtcg ctccgctacc cactgttaca gtgttaccta
     8461 atacgatacg tatccagctt tctgcagagg catcgggacc accacactta aaaagatcat
     8521 tagcaaaagt tacgccatgg ccgatgaaac agccatcacc taaaatgacg ttctcacaga
     8581 tgaaactgtg ggactgaatt cggctccggt tgccaatgat gcatcccttc tgtatttcca
     8641 caaagggacc aataaaaaca tcatccccca gttcacattc atagaggttt actggcataa
     8701 cgactttcac attttcacca aatttcacat cacgaatgcc gctgtcaata acgttcatat
     8761 ccgttgtatc ctccctcagg atgattctta tcagaacatt tcacctgtca gaatattccc
     8821 ggtaatgtga tacaagatcc agtctcaccg ggtaccacgt gttattgccc gtatcctttt
     8881 gcccgttacg agtctacaac tggttttgta ttttgcaatc caggcctgtt acaccagagc
     8941 atcctctcac ttccccctca ctggtcagtc cgggattcaa catcatcctc cagcactaac
     9001 cctataccag tcctgaacct tccctcgcca gcagcctact gttcaccaca atcctcttcc
     9061 gtgaatggca gtaagtgagc tgcaccaaaa taaatcggta cttccgatga cagacagtgg
     9121 aagtgccagg agaaatatga acatgagaat ggaagcagat aaaaataccg ttgcatgact
     9181 tcaggacggg ttatcagaat acgagctaag ggcgtcagcc aacgccctta ccgtggtata
     9241 cactcaggca ccggatacca ttttaccggg ttcacctgcc atccagtaga cacctcaccc
     9301 cacgactgca accataatta cgttgacctg aaccgtcatg cagcttcgtt tcatttttca
     9361 cgagataacg ggctctctcc agggctttct gcattgcttc cacatcacca cgcttggccc
     9421 agagcacagc ggatccaccc ggccttttat cagaatccgt ccgggtatca gaacatccac
     9481 tatgcaaagg cgtgtcatcg gaagaagggg aagcatcagc cgctgctggt ttacgggaag
     9541 gcgtccacct ggaaaaatgg accaggccat cgccttgcca gggctcagga tgcagagcgc
     9601 tgcaccatgc ctgttggatc atcgtcgcca gcgaagacgg ttcgctgaaa tccccgagcg
     9661 agcaccagat atctttgttg agcagtaata tcacgtgata atgtttcctg cctttttcct
     9721 tgccaaactc ccgacaccag aaataacgca gggtggttgc atgcacacgc tgattggcac
     9781 atcttttttt ccgctggtaa gcctgaattt tggctttcag ggatgcaaaa aaacgagaaa
     9841 tcaccgctga atccggatcg ggatccatga tggtgacagg catatactca gggaaacgta
     9901 aatccacccg gattaacgag acggcacggt gctgcgccgg ggcactgtcg agaaggctgt
     9961 caaaacggga taaccagtaa gcattgtgta ttgattgatt catgatgaga gatctccgtt
    10021 gatttggatt gacttcacat cattcatcaa cgcggtgaaa ctttacggca tcccgccaat
    10081 gagcaacgcc aatagctcct gattaaaacg gaatgaatcg tcccgtaacg aaaagataat
    10141 cctgcacacg acagcaaaac gtaaaacaac agcatgaatg agctaaatgt ttaaagaaaa
    10201 aatgtctttt cacattattt ttcagcaagt aatcatacca gcatccctaa catacgaaat
    10261 cacatagtga tcgatcgctg attcgcttaa caactgatcg aatacaccgg aatttacact
    10321 aacatcctga tgggtaacgg aagactaaaa aacctgtgtt ctattgattg cacggaatgc
    10381 ctgtgcactg gcctgaccag taagcacaat tctacgagta gtaagatatc tacgccagaa
    10441 ccatgcggca gtagccacca tatgttgcca gggtgaaaaa taagctcaga gctatagggg
    10501 agtaattccc gttaatgtca tcgctgtacc tcaatcagtt cacaaactgg ctgaaacgtt
    10561 aagctgaagc ctttattctt cagatgtatt ttccggttta cctgtaaatt atcttctgat
    10621 atttatcaca ttagtagttg tgctgattat ctacatactt caaggtctga aatatgaagc
    10681 taacagaaga aagtctgaat ctagtcattg acatcctgaa tgatttgatc tgtgaaggaa
    10741 aaatgctgaa cgatgtggag cgcagatctc tgctcaatgc agctatgaca attacagcgg
    10801 taaaagaacg ttctgtcgta tctgtatcat cccgaaaaga gcaaagtaag aaggagaaga
    10861 aggaaagaga gatcgatcct cgtttcccaa atgctggtgt ggcatggaat gaggcagacg
    10921 agagtttatt gcgagatgtt ctggaggatg taccggacga tgaaattggt aaacaccttt
    10981 tctggctggc agagaaacta gggcgaaccc cgtacagtgt agcctgtaaa gtcagacaaa
    11041 ttaagaaatt acctgcagaa tggaaggatc aatttcgtaa aatctctgac aggatccgta
    11101 gttctggaat gtcagtcagt gaatatctac aacgccaggg actgttaagc ccagagtcag
    11161 gggaagaaaa caatcctgtc agtcataaca acaatgtagc ttaatcctga agaagatgaa
    11221 cagtttatac tctttaaact gttcccttaa ttttagaaaa acagtttgtg taagtgcatg
    11281 ctccaacatt ctgaaggagt tatttcttac aatacatcat aatcattggc agatcctcat
    11341 ctgaatcaaa acaccacaac caatcatcac actttttctg aaaagagcct tatgatgaag
    11401 ataatcacct tacgctgaga aaaaacacca gggccagtta atcaccttat caatctcgcc
    11461 gttacaaaat catccataag ccatttaaca gtgttatcgc aaactcacag taaatcgcat
    11521 caggttacct caatcggatt tgagttaatt cactgggctt tacgtaaaga tacttttctc
    11581 aggttaacga ctaaaacacg aaaactcaga aaaggttaaa cacaggttca gtaaaatcga
    11641 atatcatctt gattgcttaa acaggacaga gggttttata gtgaggttaa ctgattttag
    11701 cgatcgtaaa aaaaggacta acaaatttac taaactggat gtgtatcaga caaagcaatg
    11761 tgtatagcaa tgcttaatga aaatacttgt atgattgtct atgaaagtac gtgtatatag
    11821 tatcataccg acattctcaa ctcttccaaa catccatgca gactgaatgt tggtttcagt
    11881 ttttcacggc cagcatgggg atttttgttc tgaaaacgga gcagatttac tctggaatta
    11941 ttttccctgt cgggatattt ttcacctgaa aactttctac aacacaacgg ctgtataaca
    12001 tcattctggc atattcattc aggtgagtcg gacaagcgat aatcctgttc tttaccgaac
    12061 gaacaggcag cgataaaaaa ctgattcaga cgacatttct gtctgacaaa cattcacatg
    12121 cagaagaccg ggacatttat tcttcccgaa aggaagtgat accttttctt atcatggtag
    12181 ctgcattacc tgatatcttt caggcgaatc ccctccctgt aaatctgatc gtcagataaa
    12241 agagatgaac atcaggcaga tttccgtcaa taacgataag agatgaccat cgtaaacata
    12301 tcttccagta acgaactggc acctgcatat taccgtttat tctcatacat cattgaaata
    12361 ttgtcagtta taaaggacac acatcaggaa attatcacca tgtgacaacg tcacagatgg
    12421 tgctgatata taaaaattaa caaaatcgcg ccaaaaaacc gcgccgatat tgcgcatctg
    12481 taatccatca tttccttttt gaataacctt ccgtggttac ccgacatcct gataatgacg
    12541 cccatcgtac acgcttttct ttgctcagac atcgcatact tttatcctgc tcaataccgt
    12601 cttcctgcca gtcttcccac tctctgacac tcacagaaca gcaacgtcct ccggacatac
    12661 tagcagggca gaacacaatc cacaacatta agtatatgta acaataatat accattaaga
    12721 ccacttctat gaccttatat gttacaacct tagagcatgt atttaatatc ccataataca
    12781 gactcgtcat tgttcttctg agacgacatg ggcatgtcac aaaactaatt gactgacata
    12841 tcatgaaaat gtcagtattt aataatttaa tggaggtaca aacatgagcg cagatggcat
    12901 ttccgattta ataaaaccag aaatatttct tgtcattttt cctggtacaa caaccacagc
    12961 aaaacggggt ggcgccgggt ggttgaataa taaaagagtc gtgttccggg tatggcttta
    13021 tatcagtaaa gccagttata agaagatggg gatttttcag cgcggttctg cgcccgaatc
    13081 aataccctat cgtgaatacc attttccctc ataccctgac acccatgaaa aaaggaaaaa
    13141 ggtattacgt gactggatat ccttcatgcg ctcccgtttt aatataaatc agatagctca
    13201 gttctgttat cgtatggaaa atgaatgggc attcaccttt gataatatcc acgttccacg
    13261 acactttcgg gagggaactg atgacacaga atggtcatgg aattacatta agaaaaaacg
    13321 attattttcc ctgagcggcc tgcaacacat tcagcctcag aacaatactg aacgaaagct
    13381 tctcattaat gctttttttg atacctgcct tattgaaaac aaccctgctg acgatattga
    13441 cataaagaac atgttactca gaaagatgaa tgcagcgttt tataaaagaa agagccgggc
    13501 gcgtgcgcga attcactccc ggaataacca acagatgtca gtcagttcag gaacagataa
    13561 acaggtaaat attcgtctta aggattttca gtatcagcaa cttcttttta tcgcgcagaa
    13621 agaaaacaaa agtaagtcag aaatcataaa ggaactcatt accagggcat accgggcgat
    13681 acaataacca ccacgttcac catttcatcc cgaccataca tcatatgcac gggatgatac
    13741 tgactatttt ttatcgtgcc gactgactat gccaggtata cataacgaat attcttttgt
    13801 tacatcgctt acatattcgc gtataagatc ccttttctta ttattatcac gtccactttc
    13861 tattctgaag aataattctc ttaaactaaa cctggacatt atatccgaat gcaacaatgt
    13921 aaaaaataac acatgggcca gttcttcact taacgacata tcattttcta caggcttaaa
    13981 tataacgtct cttacatatt ctttagtgtg gggaaatcta aacggcaaag aaaactgcct
    14041 tataaaaagt tcagatactc ttgtttttac accgagaatc cccttcagat actggctacc
    14101 tgtttcactc ctcctgaagt tttcatcttc cgttgaaggc aaatcctcca tcagtttttt
    14161 aacttctgac aatggatgtt tctcaagaag ataagaatat aaactaccgg gaaatgaata
    14221 cacataacga agttctgaat cccccatcag agatgagact tttttcctta tataggctgg
    14281 aactagagta tatgaataat ttattcttat actttcagaa aaatttatgg tgttaggctc
    14341 tgaataagca aaaaaaggag agacattctg agaagaaggg caaccagtac aattggctct
    14401 cgcttcaggc gggttcatta tttttgaggg aatcttaaaa acatccacat aaactccttt
    14461 acgcctcaat attgctgccg tatggtgaag catatagact tctgccactt ttgaaataaa
    14521 catcttctca tattgttctc cacaccactg actgagcgta tgaatattca tacctacagt
    14581 tctttttatc gttttttctg tgtttttcca gtatcgggtg ttaagaacag cctgtatctt
    14641 cggcggtttt ctccgcccca tcatcatatg aagttctttt gcctgctcag ataatcctgt
    14701 catttattac ctccgaatat attccattaa aaatggaaat aattatacgc ccatattacc
    14761 tgaatgataa accggattac tgtgatccgg ttcagtgaaa tcaacaggat accggtatgt
    14821 cattcagcca ttcttccctc tctgcacaag tgaaatcata tctgacgttt cttcctgaag
    14881 aaattcgcca gaaaatcctt gaacatctcc acggtgttat tcattacgag cccgtgattg
    14941 gcattatggg taaatccggc accggcaaaa gcagtctgtg taatgccatt tttcagtccc
    15001 gtatctgcac cacgcatccc ctgaacggct gcacccgcca ggctcatcgc ctcaccctgc
    15061 agattggtga acgcagaatg acgctggtcg atctgcccgg cattggtgaa acaccgcagc
    15121 atgatcagga ataccgggag ctttaccgtc agttactgcc ggaactggat ctgattatct
    15181 ggatcctgcg ggctgatgaa cgtgcgtatg ctgccgatat tgccatgcat cagtttttac
    15241 tgaatgaagg tgcagatccc tcgcgttttc tgtttgtact cagtcatgcc gatcgcgtgt
    15301 ttcctgctga agaatggaac gacacagaaa aatgcccgtc ccgtcaccag gaactctcac
    15361 tggcaactgt tacagtccgg gtggcaaccc tgttcccgtc atcatttccg gtactccccg
    15421 ttgctgcgcc tgtcggctgg aatcttccgg cgtttgtgtc actaatgatc cacgccctgc
    15481 caccgcaggc aaccagtgcg gtttattcac atatccgggg ggaaaaccgc tctgaacagg
    15541 cccggaaaca cgcacaacag acttttggtg aaaccatcgg gaaaagtttt gacgtcgccg
    15601 ttgcccgttt cagttttccg gcctggatgt tacagcttct gcgtaaagcc cgggaccgca
    15661 ttatccgcct gctggtcaca ctgtgggatc acctgttctg agcgccccgg agtcccccgt
    15721 ggcaaccgta tacctttaca cgacaacgga ttacggtaac cactgccgta taccgtctgt
    15781 cattatcaat aacacactct ttaatttcag aggtcagtca tgaataccag aattcttttt
    15841 cccctgctgt tcactgtggc atccttctcc gcctccgccg gtaactgggc tgtcaaaaac
    15901 ggctggtgcc agaccatgac ggaagatggt caggcgctgg taatgctgaa aaatggcacg
    15961 attggtatta ccggcctgat gcagggatgc ccgaatggcg tacagacgct cctgggcagc
    16021 cgtatcagta ttaacggtaa cctgatcccc acatcacaaa tgtgtaatca gcagacggga
    16081 ttcagggctg ttgaggtgga aatcggacag gcgccggaaa tggtcaaaaa agccgttcac
    16141 tccatagcag agcgtgatgt gtccgtttta caggcatttg gtgtacgaat ggaattcacc
    16201 cgcggtgata tgctgaaggt ctgtccgaaa tttgtcacat cacttgccgg tttttccccg
    16261 aaacagacga ccactattaa taaagactcc gtcctgcagg ctgcccggca ggcatacgcc
    16321 cgggaatatg acgaggaaac aacagaaacc gctgattttg gctcttacga agtaaaaggc
    16381 aataaggttg agtttgaagt attcaatcct gaagaccgtg cgtacgacaa agtgaccgtc
    16441 acggttggtg ctgacggtaa tgccaccagt gccagcgttg aatttatcgg aaaatagccg
    16501 gtatgtcgga ctgtcaccct gttttattgc ccgaaggccc tttctcacgc aaacaggcaa
    16561 tggctgccac aacagcttac cttaccgcaa tgtgcttatt gaagatgacc atggaacgca
    16621 tttcctgctg gttatccgca atgccgaagg gcagctacgc tggcggtgct ggaattttga
    16681 gtctgacgcc ggaaaacagc ttaatgtgtg gctcgccagt gaagggcttc tcagacaata
    16741 aaagtcttca tttcatccat caggccgcgt cttctccggg agacgcgcct tttcatttat
    16801 accgccaact cattcataag gagcaaatca tgcgattagc aagtcgtttt ggttatgcga
    16861 accagatacg ccgtgaccgt ccactggcac gcgaagaact gatgtaccac gtcccgagta
    16921 tttttggaga agaccggcac acctcccgca gtgaacggta tgcgtacatt cccaccatca
    16981 ccgtcctgga aaatctgcag cgggaaggct ttcagccgtt cttcgcctgc cagacccgtg
    17041 tgcgcgacca gagccgccgg gaatatacca aacatatgct gcgtctgcgg cgggccggac
    17101 agataaccgg tcagcatgtg cctgaaatta ttctgctcaa ctcccatgac ggttcatcca
    17161 gctaccagat gttacccgga tattttcgtg ccatctgcac caatggcctg gtctgcggtc
    17221 agtcgctggg agaagtccgg gtgccacacc ggggaaacgt ggtggacagg gtgattgaag
    17281 gggcttacga ggtggtgggc gtttttgacc ggatagagga gaagcgtgat gccatgcagt
    17341 cgctgattct gccgccaccg gcacgccagg cgctggcaca ggcggcactg acttaccgtt
    17401 atggtaatga gcatcagccc gtcaccaccg ctgatattct gacaccgcga cgccgggagg
    17461 attacggtaa ggacctgtgg agtgcttatc agaccataca ggagaatatg ctgaaaggcg
    17521 ggatttccgg ccgcagtgca aaaggaaaac gtatccacac ccgtgccatt cacagcatcg
    17581 acaccgatat taagctcaac cgcgcattgt gggtaatggc agaaacgctg ctggagagca
    17641 tgcgctgata aaacatatcc ctgaaggcgt gattcaggta ccagaatcac ccgacgcccc
    17701 ggaggcgctg ccggggtgtc gttccatttc tgatgatgac ctgtgcacct ggtgtacccg
    17761 tctgctttac cgtcccggag aactcagtct gtgcagactg agtacaggca atggcatctg
    17821 gccatccgtc tgtgaccgga acggttacgc gtacggctgt ccggaatttc agccgaatat
    17881 cattcatccc tgatattgcc gggtctcacg gtcagtgatg aacgtgatgt tgtcagcccc
    17941 cggcctttat ttcctctcat ttattcacat tacaaggatt tcacacatga aaacattgtc
    18001 tcagaacacc acctcttcag cctgtgcacc ggagactggc ctgcaacaac tggttgccac
    18061 catagtccct gatgaacagc gcatcagctt ctggccgcag cattttagcc tcattccaca
    18121 gtgggtgacc ctggagcccc gtgtcttcgg ctggatggac cgtttgtgcg aagactactg
    18181 cggtggtatc tggaatctgt acaccctgaa caacggcggc gcatttatgg cccctgaacc
    18241 ggatgacgat gatgacgaaa catgggtgct gttcaatgtc atgaacggta accgcgctga
    18301 aatgagtccg gaagccgccg gcattgcggc ctgtctgatg acgtacagtc atcacgcctg
    18361 tcgtacggag tgttatgcca tgacggtcca ctattaccgg ctgcgggatt acgccctgca
    18421 gcatccggaa tgcagcgcca ttatgcgcat cattgactga atggaggcag ggacaatgca
    18481 gcagctttcc tttctgcccg gagagatgac gcccggcgag cgcagtctca ttctgcgggc
    18541 cctgaaaacc ctggaccgcc atcttcatga acccggcgtg gccttcacct ccacccgtgc
    18601 ggcacgggaa tggctgattc tgaacatggc aggactggag cgtgaagagt tccgggtgct
    18661 gtatctgaac aaccagaatc agttgattgc cggtgaaacc ctcttcacag gcaccatcaa
    18721 ccgcacagaa gtccatcccc gggaggtgat taaacgcgcc ctgtaccaca atgccgccgc
    18781 cgtggtgctg gcgcacaatc acccgtccgg tgaagtcacc cccagtaagg cagaccgcct
    18841 tatcaccgaa cgtctggtac aggcactggg cctggtggat atccgggtgc cggaccatct
    18901 gatagtcggt ggcagccagg ttttctcctt tgccgaacat ggtctgcttt aacccgtcac
    18961 cgccacaatc acatcacacc tgttttcact tttatcttct gtcttcagag gtatcccatt
    19021 atgaaaatta tcacccgtgg tgaagccatg cgtattcacc aacagcatcc tgcatcccgt
    19081 ctatttccgt tctgcaccgg taagtaccgc tggcacggca gtgctgaggc gtataccggt
    19141 cgtgaagtgc aggatattcc cggtgtgctg gccgtgtttg ctgaacgccg taaggacagt
    19201 tttggcccgt atgtccggct gatgagcgtc actctgaact aatgttccgt cacccggaaa
    19261 acgagtacga ccagatgacc tcagacgccg ctgtggatga ggcccttctc atcaatgaat
    19321 acatcttcac cgaagccggt ctccacaccg gctgacctgt catcgttaac cccttcttaa
    19381 ccgattttct gtgaggactt tatcgtgtca gacacgcacc atgagacaaa ttatcccgac
    19441 gactataaca accgcccctg gtgggggctg ccctgcacca taacaccctg ttttggcgcc
    19501 cgtctggtgc aggagggtaa ccggttgtat taccttgccg accgcgccgg tatcagaggc
    19561 cggttcagcg acgcggatgc gtaccatctg gaccaggcct ttccgctgct gatgaaacaa
    19621 ctggaactca tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg
    19681 tatgccagag gactgacctg cgaagccgac accctcggct cctgtggtta cgtttatatg
    19741 gctgtttatc cgacgcccga aacgaaaaag taaatcatca gaataacctt ctgctccggc
    19801 ctggtgtttt caccacgcca ctttcccatt tttcatctct gcatatcagg aaaatcttca
    19861 gtatgaacac attacccgac acacacgtac gggaggcatc gcgctgccca tctccagtaa
    19921 ccatctggca gacactgctc acccgactgc tggaccagca ctacggcctc acgctaaatg
    19981 acacaccgtt ctccgatgaa cgtgtgattg agcagcatat tgaggcaggc atttcactgt
    20041 gtgacgcggt gaactttctc gttgaaaaat acgcgctggt gcgtaccgac cagccccggt
    20101 tcagcactag tccccgctct cagttaataa acagtattga tatcctccgg gctcgccggg
    20161 cgaccggcct gatgacccgc gacaattaca gaacggtaaa taacattacc cggggtaagc
    20221 atccggaggc gaaacgatga aactttccct gacgctggaa gccgacagca ttaacgtgcg
    20281 ggcgctgaac atggggcgca ttgtcgttga cgtcgatggt gttgagcttg ctgaactgat
    20341 taacgtggtc tgcgataacg gtcactccct tcgtgttgtt gatgaatctg accgggcctc
    20401 aacagactgc acgccaccat ttgctgccct gaccggcata cgctgcagta ccgcgcatat
    20461 cacggcaaaa gacagcgcct ggctgtactc gctgtcacat cagacgaatg acttcggcga
    20521 atcagaatgg attcatttta ccgggtccgg ttatctgtta cgtactgatg cgtggtcgta
    20581 cccggttctg cgacttaaac gcctgggcct gtcaaaaacg tttcgtcgtc tggtcgtaac
    20641 gctcatccgc cgttatggcg tcagtctcat tcatctggat gccagcgctg aatgtctgcc
    20701 gggtttaccc accttcaact ggtaaccggg acaaaagaca cagtatggaa tcattaacca
    20761 cagaaacggc gctggatatt ctgattgcgt ggctacagaa caatatcgac tgcgaatccg
    20821 gaattatctt cgacaacgat gaggataaaa cggattcggc agcactgctg ccctgcattg
    20881 aacaggccag ggaagatatc cgtacactgc gccaactgca gtttctgcaa cagaaccggt
    20941 gagtcttact caccagactt cattccactg acgccagcct gaatacggct ggcgttttcg
    21001 tttataaacc aaaaggaaaa acgatgatgt cagatatcac agcctcccgt ccggaagtgg
    21061 tcaccgggca tacggacgtt atttgctcaa ccagtatccg ccacattctg gctgtacgaa
    21121 agagtacact gctgcaaatc gacacactta tccggcaact ggctgaaatc tcagcaatga
    21181 cagaaagtat tggcggtaaa accgcactgg actgggcgat gaaacaggat tttcgctgcg
    21241 gctgctggct gatggagaaa ccggaaaccg caatgaaagc catcacacgc aatctcgatc
    21301 gcgaaatctg gcgtgacctg atgcaacgtt ccgggatgca gagtctgatg gatgcgcaga
    21361 cccgggacac atggtacagg tcactggaat atgataattt tccggaaatc agtgaagcga
    21421 acattctgag cacatttgaa caactgcacc ggagtaagga tgaagtgttt gagcggggag
    21481 tgatcaacgt cttcaaaggg ctgagctggg attacaaaac caactcaccc tgtaaatttg
    21541 gcagtaaaat tatcgtcaac aatctggtga ggtgggacca gtggggattt catcttatca
    21601 gtggaatgca ggcagatcgc ctggctgacc tggaaagaat gttgcatctg ctcagcggta
    21661 aaccgatccc cgacaaccgg gggaatatca ccattaatct ggatgaccac atacagtctg
    21721 ttcagggtaa agaggactat gaagacgaga tgttcagtat taaatacttt aagaaaggct
    21781 cagtacacat cacgttcagg aaaccagaac tggttgacag gcttaatgat attattgcca
    21841 gacactaccc ggaagccctg gcaagcacag tgaacaaaag ccgcaaggca taaaagaggt
    21901 attttacaat tcgcgaatat taactattat taattgatag caatcaatcg gcgagaaaat
    21961 caccatgcct accagtgtat ccctcagtcc ttactttgaa acgtttatcc gcgaacaaat
    22021 agaaagtggt cggtataaca atacaagtga agtcattcgt gccggacttc gtgctctgga
    22081 agaacgggaa cagcagataa agcttgaatc actccagagc gccgttaccg caggcatcaa
    22141 cagcggtgaa agtaaaagtg ctgaagaggt attcgggcga ctgacgcaca aatacaaaaa
    22201 gatggcagaa ggtgaacagc caatatgaaa ctgactgttt caccattagc agaacacgat
    22261 cttgaagcta taggtgactg gatcgctcag gataatcctg tccgtgcaat cagttttacc
    22321 gaagagcttt accaacaatg tctgttgata gccgaatcgc cagtactcta cagagaaaga
    22381 cccgaattag gggctgggat cagaggttgt tcgtatggtc gttaccttct gctgttcagg
    22441 gtactggata ctgaagttcg tatcgaacgt atcgttcatg gttcccggga tatcaacagg
    22501 gtacttgcag agccagacga caaaccatca gatcatttct ggcagccata aacaaaatct
    22561 gtgcgttaac gctccctaac acaggtagcc aggtatttta taaatctctg tattttgttg
    22621 ttacttcgat tccgcaattc agcatatcga accagacaaa atgattacag caaagcacaa
    22681 aaaagaggga ttatagcgtc aaaagaattc gaattgcaca tgggggcctc attggtggcc
    22741 tcaaaaacaa caaagacaaa acaatcgata caaaacaatt ggttatataa ccagtttcat
    22801 tccgagtccg ggcaccacta ttctaaagaa cccgctcaca aggcgggttt ttgcttttgg
    22861 atctgacaat aaccttcacg aaacaaatta gcttataaag cctgggggaa ttactctcgc
    22921 cacgttaacg agagtaattt tattgatatt aatctcctga tactttatcc ccgtccaaac
    22981 tccagccgct gcacattcac catcccagtc ttctcagcag cactgacatc aatttgtgtc
    23041 acccgcagcg catatttttc atccagtgcg tttaaccatt tcagcaggtc attaaacacc
    23101 acaggttcta tccagacctg aatattctcc ccacgctcgg ctatccgctt gatgaccacc
    23161 gagtgcgcgg aagcactgtc actgatgacc cgcgatacct gcgcaggcgt tgttgtggca
    23221 gattttcgcg ctgcaataat atccggcgcg gcgttcttca gtcgggtgtt cattgccacc
    23281 agctgctgca acatcgtctc ctgttgttct atccgttcgc tcaacggctg ccagatgagg
    23341 acgtaatatc cgacgctaaa caggaacacc accgctgcca gtaacatgcc cttttcacgc
    23401 ggagaacgcc ccgccagatg ttgtgtcagc cagtgttcgc cacggcttaa ctggcgttca
    23461 cgccattgct gaaaatagtg aataaattta tcgcgtaaca tgttatttcc tccgcaacgt
    23521 tacgccgccg gaaaccgcat caccctcttt ctgtaacgcg tcctgttgca caacataatc
    23581 tgccgccagt gcgctacgca gtttgtcgaa gctggcaaag ttcgcagccc gtagctggag
    23641 gtgaagcgtc tggcgttttt gatcaaaggt gaaaccacgc atttcgatgt cggtaagtga
    23701 cgctgatttc agggtactgg cgatcgctga caattctgcg agcagccggg tatcgtcggc
    23761 ctgcgggcga tattttttca gcgccatcgt cacctgagag cgtaaattca caatccgctt
    23821 ctgctccggg aacagtgtta agaactgttt ctccgcctgg gtgcggcttt gcgccacctg
    23881 ttcgctgacg ctccataacg tcacgccccg ttccactgcc agcgcaacca gaatcagcaa
    23941 tatcggcaga atcatcaccc gccagcgcgc ccactgtttt cggtagctga cgcggggctg
    24001 ccacggccct gttagcaggt tcccttccgg ttcgccataa gtggtaatgg cgggcagagc
    24061 cgtaacggtc aggcgttcag catctgggcc agcccatgct gatagctctt ccggtgcaat
    24121 gccgaccacg gttagtgaaa gcggtaaatc ctgctcattg agctgtgcgc ggaacatgac
    24181 cggagccagc gccagcccgg cgctccatcc ccggcattca tcgatgcggc agataacccg
    24241 ttgcgcatcg ccagccataa acccacaagg aatggacatc cagtctggcg cgacggtagc
    24301 gcgggtgatg ccgtttgcct gcaaccactg cgcaatgttg cgcatatgct gttggtgaat
    24361 cacagctacg gttgccagtt gctggtcgat tttcaacggg gcgaaatgca gttcatcgat
    24421 atcctggttc agctcttctt ccagtaaggc gggcagaata gtcggtatct gcttgcgggc
    24481 gacatcaggc agttcaacct gccagacgct gatccattcg ccgggaatgt agagtcgaat
    24541 cgcatcagtt tgcagccatt gctggagctg ttcgttgtca acgtcagacc agatgccgtg
    24601 ctccacgtcg gtggtacggt gctgccaacg gatgggatcg gaggcgcaaa gcgggataaa
    24661 aatctcaagc atggaactca ctcactttct cctgtctgat gccagagaac cgaaaagtgt
    24721 tgtgggccca tgcggacaat taacgagttc atcgtcagtt caatctcatt cacggtgata
    24781 tctgaacgca gccagaagta attgctgtcc acgctcagga cggtttttag ctgtttttta
    24841 gtacgctcat cgacgtcagc aagtagcggc tgtgcaagaa actgatcgac atcttcccag
    24901 cccttcgccg gacgttgttg taataacgcc cgcgcctgaa cagggcttaa ccacgggtca
    24961 aacagcgcct caagaatcac actttgcgcg acgtccaggg tattaatgtt gatttgctgg
    25021 cgggtcatcg gcagcgcaca gaccagcggt ttcagttttt gataaagtcc ggcgtccatt
    25081 ccctgcacca cgcgcatctc gctgatatca gccagcggtt gattagcggc gtaaaacggc
    25141 accgaacggg cgagatactc gctgtcttca cggcccagac gcgtctgcac gctgcggtct
    25201 tcgtcaataa actcccacag gctttcggct atcagttcgg cccgataagc aggcacatcc
    25261 aggcgcgtga tcagggcaat cagttgttgt accgcgagcg gacgcgacgc cgtcgtcggc
    25321 tgagcgagag cattcaggtt aaagcaggcc tgtgcgtcac gcagagtgac ggcgatttgc
    25381 ccctgcggca gtgggaaaaa acgagggccg gaagcccacg gctgcgccag atgcacgcgc
    25441 ttttcatttt tcaggctcag actgagtgcg ctcaacgcca ggctttccgc actggcgctg
    25501 taccacagcg cctgctggta ctcctgctgg ctgcgcgttc gcccaagttg ttgctgcatt
    25561 cgcccggaaa gcgtgatggt caccagcatc ataaccgcca gcaataccag caccacgacc
    25621 agtgccattc cgcgttttgg tggtgaggtg atcatgataa ttgcggcccg cgtaacaacc
    25681 agatgcgttc aatctcgccc cattgcggcg aatgcagagt catgcgcacc gccactggta
    25741 tcgcctgcac tgatgaccag ctctcctgcc agcgcgtgcc gtcgtagaac tgcaaacgga
    25801 gcgaatccgc cgggattaat ttttgcgttg ttggcttcac gctgcctgcc gcatcggtca
    25861 gtggccaggc taaccgttcg agataaccac catgaatgcg gtaaccgacg gtgagcagat
    25921 tactgcgcgg cagacgcatc aacggattaa ccacgccgcc gcgcacaaaa cgcatccctt
    25981 cactctcaga cgccagcacg ccagcgcccg ccagtaacgc tggttcacgc tggccctgat
    26041 cgcctcttac cggacgcggc atcatttgtg tcagatcgtg gttcaaaaaa ctcatcgttt
    26101 gctgcatgag gtttagtttt tgatcgtgtc cggcgacggc gctattcacg cgtgtaacgc
    26161 cgtttgtcac ctgctgcgcc atcagtgcca gtgaggcaaa aatggcaatt gccaccagca
    26221 tttccagtaa cgtgaaacca gcgcaagtcc ttctcactgt tggcctccca cggcgctaaa
    26281 ccaggcgcgt cgtgactgaa tcaccgacga aaagtcttcg tgacggctga cttcaatatc
    26341 cactgcctgg agcagcgcgt tgcctgtact cagcggtgtg gttcgccagt accagcggct
    26401 tcctgccata tcactctcgc cctggtgcct gaccgctcgt tgttcacggg tcatcaggta
    26461 aagcagtgcg gtctggtttt cagctaccca gcccgctacc gttttttctt caaggatttg
    26521 ctgattactc agcgcgccgc tggcgatctg taacaccgcc atggcggcca acgcaaaaat
    26581 cgccagcgcg agcattactt ccagcaaggt aaatccacgc ttcatggcat cccctcatca
    26641 cattgattga gggataacac gccatcgtgc gccagcttta ccgcccaaca cgcgttttgt
    26701 gccgacgcgt aaaaacgcag tgtaaacggc gtggcgggtt caaaaggcga aaaacggatc
    26761 tgcggtgtct tctttttcgc ctctttttgc agctccagtt caatatcgtg cagcgtcagg
    26821 cgacgacgct gcagctccag cgcatattcc ttctgccaga tatcacttcc gggttgtaac
    26881 agcatctgca cctgtttcgg caccgtaacc tgtgccgata accgggtcgc agaaacgggc
    26941 aaccactgcc cgtgacgacg ctgcataaac tggtaacctg gtgggtcgat tagcacaccg
    27001 agtgttttcc cttcgatcac tgccctgtcc ttaaactgcg caaagcgagt cagaaaagcc
    27061 tgcgccgctt ttttcgcagg cggctctgaa gcggtcgcaa acgtctgtac cacgcccgca
    27121 ctggcaaggc cgataaggaa aatcaccagc atgatttcca gaagtgtgaa tccgcgttca
    27181 ggcactgatt actgaaactc ctgcaaattc cagttaccga tatctgcgcc agcgccctcc
    27241 ccgttttcct gaccatctgc ccccagggta tacacatcaa acagtccttt ttcacccggg
    27301 ctgagatact gataatcatt gccccacgga tcttttggca gtcgtttaat gtatccaccg
    27361 gtacggtagt tgcgggagtc cgccatattg gccggttgct ggatcagcgc ctccagtccc
    27421 tgttcggtag tcggataacg cccgttatcc agtcggtaca tatccagcgc attctccagc
    27481 gccacgatat cgctgatggc tttttgccga tcggctttct ctttgttgcc cagcaggtta
    27541 ggcaccacca gacttgccag gacgccaaga ataacaatca ccaccatcac ttccagcagg
    27601 gtaaaacctg cccgtggttt ttgtgtgcgg gataacgaat tcatgtgata actccgtaaa
    27661 ttacattcca accatattgt tcagttgcag catcggttcg aggatggcga tgacgataaa
    27721 cagcaccacg cccgccatca tcaccaccag cgccggctca aacagcccta acgccagccc
    27781 cacctgggta tcaaattctc gttcctggtt aactgcggcc tgttcaagca tggtttccag
    27841 ttcaccgctc tgttcgccgg aggcgatcat gtacagcatc atcggtggga acaggcgcaa
    27901 ctccgccagc gcagcgcgca gactgcttcc ttcgcggacg cgatctgccg ccagcagcag
    27961 ttgttgttcg acatagcgat ttgccgacac ggcggcagcg gtctgaatgc cttccagtag
    28021 cggaacactg ctggcggtga ggatgcttaa cgtgcgggaa aaacgcgccg tattcagccc
    28081 gcgcgcaaca cgccccgtca cgggcaaacg cagcaaggtt ttatcccagc gtaggcgcat
    28141 ggcaggattt ttgagtagcc gttgccccag caccagaagc cccagcaatc ccgccagcca
    28201 gtaaacgccg ctggtctgta acgtgtcgct catggcgatg agcgtgcggg tggaggcagg
    28261 cagcgcgtgt ccgagatgat caaactgttc gatgattttc ggcaccactg ccgtcagcaa
    28321 aatagtgact acgcccgttg ccaccaccag caataccagc ggatagagca tggcctgcaa
    28381 cagtcgtgat ttcaggcgct gtcgctgttc ggtgtaatcc gcaaggcgat tgagcaccac
    28441 gtcgagatgc ccggattttt ctccggcagc gaccatcgaa caaaacaggg aatcaaagac
    28501 gcggggatgt tcgcgcaggc tgtccgacag ggtataacct tcctgaatcc ggctgcgcag
    28561 cgccattccg aggcttttca catgcagttt ttcactttgc tcactgaccg cctgtaagca
    28621 ggtttccagc ggcattgctg cctgcaccag cgttgccagt tggcgcgtga acagcgccaa
    28681 atctgccgcc gccacgcgac gatgtgcgtg ccgccgacgc tgcaacattc cccctgtcga
    28741 agcattcagc cgggcttcaa tgtgcacggg gataagctct ttaccacgca acaactggcg
    28801 ggcatgacgc gcggaatccg cctcaatcat tcctttggtt ttgcgaccat tacgctccag
    28861 cgcctgatag taaaacagtg ccattacgcc tccatagtta cccgcagaac ttcatcgaga
    28921 gaggtttctc cggcgagcac tttctcaatg ccgttgctgc ggatgcccgc agagtgttgc
    28981 cggacataac gttccagctc cagttccccg gcctgacggt ggatcaaatc acgcaatgtg
    29041 gcgtccacca cgatcaactc atggatggca gtacgcccgc ggaaaccttt gtgattacag
    29101 gcggggcagc cctgcggatg gtacagcgtg acagtacggg cgtcggtaat ccccagcagg
    29161 cgtttttctt cgtcagtggc aggcgcgggc tggcggcagt cggggcacag cgtgcggacc
    29221 agtcgctgcg ccatcacgcc cgtcaggctg gaagagagca ggaaaggctc aacgcccata
    29281 tcctgcagac gtgtgatcgc ccccaccgcc gtgttggtat gcagcgtgga aagtaccagg
    29341 tgccccgtga gcgacgcctg aacagcgatt tctgcggttt ctgtatcgcg gatttcaccg
    29401 accatcacca catccgggtc ctgacgcaaa atcgcgcgca ggccacgggc aaaggtcatg
    29461 ccgacgcggg tgttgacctg cgtctgaccg atcccttcaa tcatgtattc gatggggtct
    29521 tcaaccgtga gaatgttgcg cgaatggttg ttcagctcct gcaatccggc gtacagcgtg
    29581 gtgcttttgc cggaacccgt cggtcccgtc accagaaaga tgccgtgcgg tttgtgtaac
    29641 agctggcgca actgcacggt cagttgctga ctcaacccca gacgttccag cgtcaggcgg
    29701 gcctggtttt tgtccagcag tcgcagcacc acgcgctcgc cccaggcgga gggcatggtg
    29761 gagacacgca cgtcaatcgc ccgaccgccc agcagcagcg caatacgccc atcctgcggt
    29821 acgcgctttt cggcaatatc cagccgcgcc atcaccttga tacgcgatac cagcagcgag
    29881 gccagtttgc gccccgggcg cagcatttca tgtaatgtgc cgtcaacacg aaaacggatc
    29941 accagactct tttcaaatgt ctcgatgtgg atatccgaag cgccttcttt aatcgcctct
    30001 gccagcatgg cgttgatcag tttgatgatt ggcgcgtcgt cgtcgctttc cagcagatct
    30061 tccgtttccg gcagttcttc ggcgagggta aaaaaatcct cagcagaacc gagatcttcc
    30121 atcagctggc gggcctcgga agagtcccgc tgccagaccg cattcagccg ctgttcaaat
    30181 tcggcttcgt cgatttgccg cagcgtaaag ggcgcgttca gcccccgctg cagctcctgc
    30241 aaaacagaga gcgacaacgg atgaacgtgg aggatctcca gcgacgcttc gcaccatgcc
    30301 accaggctaa accgacggct gaaactgtag ggcagacgca cggtgttagc ggtggtttcc
    30361 tgtgctacag gcaccattaa cgcgttctcc cggcattgag gaatgcgcga acttccggcg
    30421 gtaaggcctg gttttgcgct ggcagtaccg gctgcgcggt gtgcggcatc aggcttaagc
    30481 cttgatcatc gcgatagatc tgctcggcgc gcatgtagtt atatttgcgc tgcgacacgc
    30541 cgtctgccgc cataccgtca cgcagaatgg tcgggcggat aaacaccatc aggttacgtt
    30601 tttctttttt atccgccgtc gatttaaaca ggttaccaat caacgggata tcgcccagca
    30661 gcggcacttt cgccacgctt tctcccgcct ggtcgtccat cagaccgcca agcacgatca
    30721 gttcaccatc gttagccagc acggtggtct tcagtttgcg ctcaccaaac accacgtcga
    30781 ggctggtctg tccttccacc ttcgacactt cctgctcaat caccatctgt accgcgtttc
    30841 cttcgttaat ctgcggcgtg actttcagca tgatgccgac ttttttcctc tctaccgtat
    30901 tgaaaggatt gctgttattg gagccaacgg tagatccagt taataccgga acgtcctggc
    30961 ccaccatgaa gaaggcttcc tggttgtcca gcgtggtgat gctcggcgtg gagagcacgt
    31021 tcgagctgga gtcgtttttg accgcctgta ccagtgccat ccagtcgcct ttcaccacgc
    31081 caaccgccgt accgctaaag ccagaaagca gctgggcgag cgtggagaga tcgccgttgg
    31141 tatccgggtt aatggtggtg gcaccgtttt cactgatcac cgtggagcct ttctgcggtt
    31201 tcgcctgaga aatggctgcc cccagcgtgc cgatagggat ctgcgtaccg ttagcaaact
    31261 gcattaatcc ggcatctttc gacgcccact gcacgccgaa attgatattg ctgccttcag
    31321 caacttccac gatcaacgcc tcgacgtgca cctgagcacg gcgaatatcc agttgttcaa
    31381 tcacgctttg cagcgactgc atgatgtcct gcggcgcagt aacaatcagg gcattactgt
    31441 gtttgctggc ggcgatggag acaacctcac gcccgctacc gacggtgcct tccgcctctt
    31501 ctttagccgc cgtgagcgtg ccgctgacct gcttcagcac atcgaccaga tcttccgctt
    31561 tgctgtattt gagatagaaa acctggctgt tgccgctgcg ctccatttct gagtccagcc
    31621 gacggatcag gcggcgcatt ttgtcccgcg tggccgggtc accactgaca atcacactgt
    31681 tggtgcgttc gtcggcgaca atttgagatt tcagcgtcgc aggttggttc tcgccgctgt
    31741 ttttcgtcag gctttccagc acgcgggcga tttccgaagc cgaggcgtta tccagcggga
    31801 tcacctcttc agtgcgatta cccgcgtgat ccacacgctg gatcacttcc gtcagccgct
    31861 ccacgacgga ggcgcgcccg gtgagcataa tcacgttgga gggatcgtaa ttaacaacgt
    31921 tgcctgagcc tgcgctgtca atcatctggc gcagaatcgg tgccagctcg cgtaccgaca
    31981 cattgcgtac cggcacgact ttggtaacca tttcatcgcc cgcgtagttg tcgctgcctt
    32041 caccgaccag cggcagcggc tcgactttcg cggcgcttga tttcaccacc ttcagcacgt
    32101 cgttttccat cggtacgacc gcatacccct gtgcttccag caggttaagg aataactggt
    32161 aatactggcg ttcattgagc ggagtcatag tgcgaatact cactttcccc tgaacgcccg
    32221 gccccataat gatggtttta ttaaggttag cgccgacggt ttcgatgaac gatttcaggt
    32281 cagtatcttt aaaattagcg gtgaaagtgg cttcttccgc ccagaccggt gaactacaca
    32341 atgccgctgc cagcatcagc ggcagtaaac gcttttttgt tttgaggcca gttgtcttct
    32401 tacgccagat agacaacgtc atatcacgcc aaaacacgat gaatgattct cctggattaa
    32461 atgcggttag cgcagtgcga tggaaatgtc gtagcgcgca cccttgcgta aaaccgtaag
    32521 ttgaatggaa tccattgaag gtaactgccg catcagagca atcattgctc gtggatcagt
    32581 gaaatcctgc tgatttagtg caatggcgat atcgccttcc ctgaaaccgc tggcatcgaa
    32641 cagagaacga tctgcccccg gcttcactgc ataaccgaca attccctcct tacgcacagg
    32701 cgtaagctgg atatagttaa aaattttctg cggatctttc gccagtgcct gacgcacggc
    32761 agccgggatc tcaactggcg cactggcagc aggctcagca acagcttgct ttgcttcgtc
    32821 gctgacggct tttttgctgg tgaccgccac aggggggcgt tcctcctctg ccagactcag
    32881 acgttccatt ttcccctgat agcgcagcat cacatggtcg cggttgattt cctcaatcac
    32941 tgcgttgtga gagccaagcc gttcaccctg caaatagacc tgctgtttgc cgccttcttc
    33001 aatcaccgcg ccgggtctgg caccaaaggc gatcccacgc agcaccacat taagacgcgt
    33061 ttctgccaca ggcgcagatt caggttgttt taccggtgcg gcgacgggtt gatatttgcc
    33121 aaaccagttt tgttggctga ctaattgcac gtcattttta tcgaacggtt ttgcatccgc
    33181 acggagcggt ttattcgccg atgaggaaac caccgtatat tccgcagaaa aggagaaata
    33241 gcgccagagt gaatacgcca tttttgcaga aataataagc agcatgagcc agaacatccc
    33301 gcgcaccatt tttcgcagat gctctttgtc tgtattaaga gattgtctct ggctaagaat
    33361 gtgacgaatt ttagtcagcc attgaatgag ataaattcgt gcgtcacgaa aaacaacccg
    33421 cgccaattta tatctacccg acgttatgct ttgactattc cacaggtggt acgatccagt
    33481 ttccgctgat actggttacc tgtacgtgta tcattaatcg ttatgctgta attaattccc
    33541 tcggtgatca ttaatttcac cgttgggtca gcgcacattt gtcgttgata gctggttaaa
    33601 aaggcgtcag gtgtctgcgt ggtctgagta cccgattcgc tgataatggt catttttacc
    33661 gtcgtgccac tactttgcgc cagcactaag gtatatcccg cagatttaat cggcaggttt
    33721 tggctgatat tttgcgcctg ttttttcgcc agtaaactgg cgttttcatt atggctggcg
    33781 cagccactta ataatcctgt aacgctcaac aatagcgata ttaataccct ccctggcatt
    33841 tgttttatcg acatggtaaa taatctctaa ggttattaat aagagttaaa atgtcacttt
    33901 gataatgacg ttgttatcat taaaacaatg cctttagata aagtgttgct ataccgccca
    33961 gacttaaaca cggtccaaaa ggcagtgtgg ttgatcctct ttttgtaata acggcatata
    34021 tcaggccgca gcatgaggcg attaaagcaa cgttgggcag tgataacgcc cccacccagc
    34081 cacctaaagc ggcgaagagt aatacatcgc ccatgcctaa tgcttcttta cgcagaacta
    34141 ttccggctat ccagcgcagg gagtaaaaag tgataaatcc caccagtacg ccggtgactg
    34201 cgtcttgtag cgtgagcgga ctctgctgcg cccatgccgc aattagtccc gtccacaata
    34261 cgccctgagt aaaaacatcg ggcagccatt ggttatcgag gtcaatgacg ctcgcggcaa
    34321 tcagccaggc ggataatatc atcaccgcca gcgcccatcc actttccggc cacaccaggc
    34381 tcgccagcaa aaaagcgagt gccgtcaata actccaccag cggataacgc ttgctgattt
    34441 tcgcctgaca gtcgcggcag cgccctttga gcatcaacca ggagagcagc ggaatattgt
    34501 cacgaacgcg gatagtctgc tggcaatgcg gacagtgcga gcgtggcagc gccaggctta
    34561 tttttgactg cgtactcggc atttcaccgt gaaactccgc catttgttgg cgcagcatga
    34621 tggggtaacg ccaaatcacc acattcaaaa aactgccgat aatcaatcct ccgacggtag
    34681 ccaggacggg catcgccgcg gggtattgct gaaaaacatc aaaaaacatg gttaaaggtt
    34741 atttgttgta acttgccgga tgcgggcctg caggtgtatg ccatacggct ttcctccagg
    34801 cccgatgcgc cttatttcat gccggatgcg gcgcgagcgc cttatccggt atacaggctt
    34861 actcagctga catcttatgc tcggtaacct gattaatggt ttccggccct tgctgcggtt
    34921 tcggcagatt aagcgccgcc agcgtctcgt aagccgactg gctcacaccg ccctcgaagt
    34981 tcatctcgct cgctcccggc aactggtaag cattcgcgcc cggattccat ttcttaaaga
    35041 actccgaaag atccgtctgg gcgacccagg aggcacacag catcagcgtg tccgctgcgt
    35101 taccgttgga ttcagcacag tagttcttac tgccgaactt gttattgcca acctcatcgc
    35161 cgcgcgcttt acggtgcatc aactggaaca ggttccagcc tttcatccct tcacgctcgc
    35221 tgtaaaactt aggcaggtca ccttctgggt accactgttt gatatcaaag tttttctctg
    35281 cccactcttt cagctgtgcg tacatcagca gacggtcccc cgcaccgccg cgcgcccagg
    35341 cctgaccgtt gctctcgtcc agatattccg gcgcgacggt aatgtcgtca gcgacacggt
    35401 tcatcttgcc gagatagcga tcctgcatgt acagcgccag cacgttgttc gccacttcag
    35461 ttgcacccgg tacgttcagc ggtgtttctg cagcgttatg accggcttcg tgccaaatca
    35521 gccagtcgtt cagcggcgtt gtcggcagcg tggtgctgtt cgtcgagaag ctgctgttca
    35581 ttaccggata acccgagtgc gcatcaccga tggagatctg cacatcgttg gcgaaacggt
    35641 gtttatgccc cgtcaatgct tcataggtaa acatccggtg gttaccgctc gtttcattgc
    35701 gaccgtagaa gtcattcatc gagctggcaa aggtatccag atctttagcg aattctgcta
    35761 caccaccagt gaaattgctg gcctcaaggt tcttcttcgg cgtggtatag acgaacgacg
    35821 cagactccag ctcgcccagc ggcgccggtg agtccagatc gtttttccat tcgccgtctt
    35881 tatagaacgg cgcttttact acaccggtaa aggtgaagtt agcagaaaca tcatccttac
    35941 tgtcgccctt gatataaatc agaccaccat aaggcacctt gaaggtcact tcaccgttag
    36001 cctccagagt atacgtttta gtcactcttg gcggacggtt cagcgcaact tcatgcttct
    36061 cacgtccagt caggtcgtca gccagcgcca cggtaacagt cactgggact gacgccgaag
    36121 acttaatggt gacgtcctgc tgggccggtg cccacaggcc ggttgactgc atgttacccg
    36181 caaaccattt ggtcggattc gagtacaggc tgatgttttc cgtaacgctc tcaccctttg
    36241 ccgatacgga tcctgggtac ttctccacat caaccttaat gttcagatcc caccaggaac
    36301 ggcccagcat cagacgcgtc agcggttttt ccatatagtt gagcggatag cttgggttca
    36361 tcatgcccgc tttgctgcta ccgtcaccgt agatcatgtt gttatcgacc agcgattttt
    36421 tcagatctgc ggagcacttg gtgccgcctg cataggcatc attggcgtag cagttcagga
    36481 actcggtgaa cgttttaaag cccagctcat cttccttgcc ctcttcgtaa cgatatttcg
    36541 tatcgttcca cagccagacc gacatgttct ggtacagacg ttccagatca acactgttca
    36601 gacgctcacc tttactgcct ttggtacgga aataaagctc atgctgatac aggcgctgaa
    36661 tgttggtgcc taaatccgcc gcctgcacca tcgctttcgc ggtgtcggcg tcaagattca
    36721 gttgcgtata gcgcggaaca tacatgccac ctgttaccgg aacatccgtg cctgggcggc
    36781 gctccaaaca gttaatctcg taatggtaag tcgagtcctt acactcctgt aacccaggaa
    36841 acttctcaaa gatttttgcc tttgccgctt ccagagattc ttctgttgtg tattccgctt
    36901 catcaataaa ggcataacgc gttacctgtt tgccctcaac ttcctcctgc cagctcgcaa
    36961 cttccagttt cggcttgtca tcaggcttgt tgtcttgctg gtatttccag gtcacttccc
    37021 ctgtatttgg gtcgatggtg tacggcggtt gcgcgccgtc tgcagcagga taacgttcat
    37081 aaacccaaat gccagtcgcg cgacgctgac gaacgcgatc cggataccct tgcggatcgt
    37141 tgttcaccac cgatttgttc agagccattg acagacccgc ggcatccagc agacgcacaa
    37201 aactggacgc gctctcttcc ttaagattgc tcatcacgtt ttccatgatc agcaccgagc
    37261 cacctttgtt cagataagcg atcagatcgg tcacatcctg ctgagtcagc ttcggtttgc
    37321 tggtatctgc acgcagaggc acagcatagg gatcgccaga ccactgagtc acatattcaa
    37381 agccgttgag gatcagcaac ggaatctctt ccggattcag atcgccatag ctggtcaact
    37441 gtttaaccgt gataccagtg aaatcctcat ggaaagcaaa tggtgcacta tttcccaata
    37501 cctggcccgc ttttttgaaa taaacgttct ccaggttggt gccgacagtc atgatgctct
    37561 tggtatttgg ctgccagatg tcatttgaca agtagcgcag gacgttctgc ataaagtgct
    37621 tcatgtcatc agaatcaccg ctgagcgtac actcaccttt actattaaca ccaccgcccc
    37681 aactgtaacc gttcgggcaa cgcaggatgc tgttgtagtg cgggttaccg ataaccatca
    37741 gtttgccttc accgacttgc cccagcgaaa taaacggcag gttgaaagtc gcagtatcgc
    37801 gcgtaacgtt ctctggctgc acaatggaag gcgcttccgt aatgtacgcc agctcatttt
    37861 tatcccaggc gcgtttttcg ccaaacgcca gccagtagtt tttatcatta cgcgccatca
    37921 gaatcgggaa tgccgagttg gagatattta ccaccgcctg accgcgcgcg ttaccggtgc
    37981 tgccatagaa gttggtagag tcatggaaga cgtggaactt gctgacagac tgatagttcg
    38041 tatccacgcc ccacagcttg ttaataacgc cctgaatctg gccgtcatta acattgcgcg
    38101 ttgtcagcga gaaccagcga gcctcgttac aaccgtcggt tttcgcacaa atcgcggtat
    38161 cgatctcttt ggcctgaccc gtcttaaact gctcgataaa ttcgttaggc agcacaacgt
    38221 tttgatcgcc ttcatccagc gtcgcaccgt tggataacga aagattgatt atctcgttga
    38281 tcacgttggg atattcggca aagaccttgc gtacatagtc cggaacaaca cgagtattat
    38341 tttgaccagt cgtcgaataa cgatgaatga gctgatcgat atttgccccg cgaacttcat
    38401 cacccaattc agtcagcgca atggtcgact tattgccacg tactgagccc agttcaaagg
    38461 tatcgatacc aaaggagatg tttcgcccca gctaaaggag aatttaccgt tttcgtcagt
    38521 tacgccacgg cctgaattgg tgtagtagtc aacgccagca acaccgttcc ccaggctatc
    38581 caccagttgg ccttcggaaa gaatgatttc agtgggttga tactgataaa actgttccgc
    38641 gttagccgac acgaaggacg cgttcagatc cggttttgtt ccctctgtcg tgactggcac
    38701 taccgttgag gtatgggtgg acggcgcttt atcagtcgca gcattgtttt ccacctcttc
    38761 attgaccagc ttgctgaaat tgtctgttgc cagatcaatt tgcttataca gtttttcaaa
    38821 tcgcgcgcga tcaaccactg acgagaaagt aagacaaagc tgttctgcat ctgcggggca
    38881 actgtcgctg gacgtcacca gagagatggc gttggttttc ttattttcag aattcgccag
    38941 ctcctgcgcg tcctccaggc taaacgacac tttgtcaacc gcacgcaggc tacgcgcagc
    39001 ttctgactgg gtgttgaatg ttgcaatggt cgtactgccc accacacaac tcacggtatt
    39061 gcctggcgta aaggtaaagc catcgctgga ttcaccatta caggtagcac cagttacccg
    39121 ctggcttccg cccagggtca gataacccgt tttcgtagga acaggttctg gctccggttc
    39181 tggctctgga tcaggtgtcg gttccggcgt aggttctggg tccggcgttg gctcaggcgt
    39241 cggctccggg tttggtgttg gatcaggttt cacttccggc aaagaccctg ttccagaatc
    39301 tacaggcggc gtatcggagg aagatccgga accaccgcca t
//
LOCUS       DAZGTJ010000037        39335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000037 DAZGTJ010000000
VERSION     DAZGTJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.037"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(20..565)
                     /gene="hemG"
                     /locus_tag="ACHK8E_002400"
     CDS             complement(20..565)
                     /gene="hemG"
                     /locus_tag="ACHK8E_002400"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP0344657.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            complement(577..2028)
                     /gene="trkH"
                     /locus_tag="ACHK8E_002401"
     CDS             complement(577..2028)
                     /gene="trkH"
                     /locus_tag="ACHK8E_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP0344658.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2067..2681)
                     /locus_tag="ACHK8E_002402"
     CDS             complement(2067..2681)
                     /locus_tag="ACHK8E_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP0344659.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2681..4012)
                     /gene="pepQ"
                     /locus_tag="ACHK8E_002403"
     CDS             complement(2681..4012)
                     /gene="pepQ"
                     /locus_tag="ACHK8E_002403"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP0344660.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            4202..6391
                     /gene="fadB"
                     /locus_tag="ACHK8E_002404"
     CDS             4202..6391
                     /gene="fadB"
                     /locus_tag="ACHK8E_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP0344661.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMEQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            6401..7564
                     /gene="fadA"
                     /locus_tag="ACHK8E_002405"
     CDS             6401..7564
                     /gene="fadA"
                     /locus_tag="ACHK8E_002405"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP0344662.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(7756..8457)
                     /gene="fre"
                     /locus_tag="ACHK8E_002406"
     CDS             complement(7756..8457)
                     /gene="fre"
                     /locus_tag="ACHK8E_002406"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP0344663.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8503..9996)
                     /gene="ubiD"
                     /locus_tag="ACHK8E_002407"
     CDS             complement(8503..9996)
                     /gene="ubiD"
                     /locus_tag="ACHK8E_002407"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP0344664.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10163..10651
                     /gene="rfaH"
                     /locus_tag="ACHK8E_002408"
     CDS             10163..10651
                     /gene="rfaH"
                     /locus_tag="ACHK8E_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP0344665.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10648..11430)
                     /gene="tatD"
                     /locus_tag="ACHK8E_002409"
     CDS             complement(10648..11430)
                     /gene="tatD"
                     /locus_tag="ACHK8E_002409"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP0344666.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11472..12248)
                     /gene="tatC"
                     /locus_tag="ACHK8E_002410"
     CDS             complement(11472..12248)
                     /gene="tatC"
                     /locus_tag="ACHK8E_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP0344667.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12251..12766)
                     /gene="tatB"
                     /locus_tag="ACHK8E_002411"
     CDS             complement(12251..12766)
                     /gene="tatB"
                     /locus_tag="ACHK8E_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP0344668.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12770..13039)
                     /gene="tatA"
                     /locus_tag="ACHK8E_002412"
     CDS             complement(12770..13039)
                     /gene="tatA"
                     /locus_tag="ACHK8E_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP0344669.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13118..14758)
                     /gene="ubiB"
                     /locus_tag="ACHK8E_002413"
     CDS             complement(13118..14758)
                     /gene="ubiB"
                     /locus_tag="ACHK8E_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP0344670.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14755..15360)
                     /gene="ubiJ"
                     /locus_tag="ACHK8E_002414"
     CDS             complement(14755..15360)
                     /gene="ubiJ"
                     /locus_tag="ACHK8E_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP0344671.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15374..16129)
                     /gene="ubiE"
                     /locus_tag="ACHK8E_002415"
     CDS             complement(15374..16129)
                     /gene="ubiE"
                     /locus_tag="ACHK8E_002415"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP0344672.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16224..17651)
                     /gene="rmuC"
                     /locus_tag="ACHK8E_002416"
     CDS             complement(16224..17651)
                     /gene="rmuC"
                     /locus_tag="ACHK8E_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP0344673.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17868..18635
                     /locus_tag="ACHK8E_002417"
     CDS             17868..18635
                     /locus_tag="ACHK8E_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP0344674.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            18659..19663
                     /locus_tag="ACHK8E_002418"
     CDS             18659..19663
                     /locus_tag="ACHK8E_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP0344675.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            19653..20282
                     /locus_tag="ACHK8E_002419"
     CDS             19653..20282
                     /locus_tag="ACHK8E_002419"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP0344676.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            20307..21281
                     /locus_tag="ACHK8E_002420"
     CDS             20307..21281
                     /locus_tag="ACHK8E_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFP0344677.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            21298..21804
                     /locus_tag="ACHK8E_002421"
     CDS             21298..21804
                     /locus_tag="ACHK8E_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFP0344678.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            21817..23307
                     /locus_tag="ACHK8E_002422"
     CDS             21817..23307
                     /locus_tag="ACHK8E_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFP0344679.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(23341..24105)
                     /gene="udp"
                     /locus_tag="ACHK8E_002423"
     CDS             complement(23341..24105)
                     /gene="udp"
                     /locus_tag="ACHK8E_002423"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP0344680.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            24367..25179
                     /locus_tag="ACHK8E_002424"
     CDS             24367..25179
                     /locus_tag="ACHK8E_002424"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP0344681.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            25571..26704
                     /gene="tssD"
                     /locus_tag="ACHK8E_002425"
     CDS             25571..26704
                     /gene="tssD"
                     /locus_tag="ACHK8E_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP0344682.1"
                     /translation="MSDIIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            26701..26958
                     /locus_tag="ACHK8E_002426"
     CDS             26701..26958
                     /locus_tag="ACHK8E_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0344683.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            27033..27278
                     /locus_tag="ACHK8E_002427"
     CDS             27033..27278
                     /locus_tag="ACHK8E_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0344684.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(27447..29708)
                     /gene="metE"
                     /locus_tag="ACHK8E_002428"
     CDS             complement(27447..29708)
                     /gene="metE"
                     /locus_tag="ACHK8E_002428"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP0344685.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            29826..30779
                     /gene="metR"
                     /locus_tag="ACHK8E_002429"
     CDS             29826..30779
                     /gene="metR"
                     /locus_tag="ACHK8E_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP0344686.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(30667..31566)
                     /gene="bioP"
                     /locus_tag="ACHK8E_002430"
     CDS             complement(30667..31566)
                     /gene="bioP"
                     /locus_tag="ACHK8E_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP0344687.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(31642..32442)
                     /gene="yigL"
                     /locus_tag="ACHK8E_002431"
     CDS             complement(31642..32442)
                     /gene="yigL"
                     /locus_tag="ACHK8E_002431"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP0344688.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(32450..33472)
                     /gene="pldB"
                     /locus_tag="ACHK8E_002432"
     CDS             complement(32450..33472)
                     /gene="pldB"
                     /locus_tag="ACHK8E_002432"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP0344689.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            33583..34203
                     /gene="rhtB"
                     /locus_tag="ACHK8E_002433"
     CDS             33583..34203
                     /gene="rhtB"
                     /locus_tag="ACHK8E_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP0344690.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(34265..34885)
                     /gene="rhtC"
                     /locus_tag="ACHK8E_002434"
     CDS             complement(34265..34885)
                     /gene="rhtC"
                     /locus_tag="ACHK8E_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP0344691.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(34949..36778)
                     /gene="recQ"
                     /locus_tag="ACHK8E_002435"
     CDS             complement(34949..36778)
                     /gene="recQ"
                     /locus_tag="ACHK8E_002435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP0344692.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(36910..37779)
                     /gene="pldA"
                     /locus_tag="ACHK8E_002436"
     CDS             complement(36910..37779)
                     /gene="pldA"
                     /locus_tag="ACHK8E_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP0344693.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            37944..38411
                     /gene="yigI"
                     /locus_tag="ACHK8E_002437"
     CDS             37944..38411
                     /gene="yigI"
                     /locus_tag="ACHK8E_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP0344694.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            38463..>39335
                     /gene="rarD"
                     /locus_tag="ACHK8E_002438"
     CDS             38463..>39335
                     /gene="rarD"
                     /locus_tag="ACHK8E_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP0344695.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQ"
BASE COUNT         9562 a        10464 c         9944 g         9365 t
ORIGIN      
        1 cattttttat tctttatgct tatttcagcg tcggtttgtc ggttaaatgg gcgatttctc
       61 gggcgaaatt cgccacctgc tcccaatcgg tatagacaac ttctttgcgc gtatccgttt
      121 caccgcctga catcttcata atcagcttga tcataaaacg gtcgtaccag cgataacgtg
      181 ggtaacgcag cgccccggca atgacggcac agcgatcggg acgccattgt gaactcatca
      241 gaaacttccg cgcgtagctg ttggtctgtg gagtacgctt ctccggttta cgcgccacca
      301 ggtttacgga gtaaaaggcg ctcggcatcg aattcagtcg cgtcgcatgc tttttgacaa
      361 actcctggaa cgcagagtgg tagtgaccat agcgaataga agcaccaatg accacacggt
      421 cgtagttttc ccactgtggt tcttcaatgc ggtgcacatt ggcgacatcc gcctggatcc
      481 ccagttcttt cagttccgaa gccaggtagg aggcaatctc gcgcgtttgt ccgtcccttg
      541 ttgagaaaag aattaatgtt ttcacgtatt actccatcat tcacgccaga aagtcggggt
      601 aaagagcacc agcaatgtaa agacctcgag acgaccaaac agcatgttgg caatcaggat
      661 ccatttagcc accgggttca tactggtaaa gttatcagca accacaccaa gccctggccc
      721 caggttattc aatgtcgcaa caaccgaggc aaaggcagaa aagtcatcca cgcccgtggc
      781 gataatcgcc agcatactga caataaacac caatgcatag gcggagaaaa atccccacac
      841 ggcttcgagg atacgttccg gcagtgcgcg attccccagc ttaatgctat agacggcgtt
      901 cggatgcacc agtcgtttca gctcacggtt cccctgctta aacagcagca ggatgcggat
      961 cactttcagg ccaccgcccg ttgacccggc gcaaccgccg ataaaagctg aacataagag
     1021 cagtaccggc aaaaagagcg gccagcgggc aatgctgtca gtcgtaaacc cggcggttgt
     1081 cgccatcgat accacctgga aaaacgcctg gttaattgtc atcagcgccg aactgtagac
     1141 attatgaaac cacagtacga gtgtacaaat aaccaccagc gtaaactgta cgccgataaa
     1201 catgcgaaat tccggatcgc gccaataaac cttcagacta cgcccactta acagtgaaaa
     1261 gtgcagaccg tagttacagc cggagatcag caggaagata gcaatgatag tgttaatagt
     1321 cgggctgtcg aaataaccga tactggcatc atgtgtcgag aagccgccaa tagcgatagt
     1381 cgcaaagcta tggccgatgg catcaaaggc atccattccg gcaaaccaca acgccagcgc
     1441 acaggcgacg gtcagcaaga cataaatcaa ccacagggtt ttcgccgttt ccgcaattcg
     1501 cgggcgcatt ttgttatctt tcagcgggcc gggcatttct gcgcgataga gctgcatccc
     1561 acccacgccg aggataggca gtatcgcaac cgctaacacg atgatcccca tcccgccaaa
     1621 ccattgcagc atctggcgat aaaagaggat ggcgtgaggg agcgaatcca gccccactag
     1681 cgtagtggcc cccgtggtgg tcaggccaga gaaagattca aaaaacgcat cggtaatcgt
     1741 gaggttcggg ctttccgaga agataaaagg gagcgcaccg acgctaccca gcacggtcca
     1801 gaacagcacc actatcagaa acccctcacg ggatttcagt tcgcccttct ctttgcggtt
     1861 cggccaccac agcatagagc caatggcgag ggcgacaaaa aaggtctggg taaaagcgcg
     1921 gcccgctcca tcccggtaga tgagtgctac cagcccaggg ataatcatgg tccctgaaaa
     1981 taagatgacc agtagtccaa cgattcgggt aatggcgcga aaatgcatct ctgccgcttc
     2041 cttagttctg caaaacgaag tggggattat tcttcaatcg ctaacaattg caatgaacca
     2101 cggctaaaat ccgccagctt tgcggaaaat tcagccactt tcgccgccgg aagcgccacc
     2161 cgcagcagaa cgaatgcctg ataatcactg ttaataattt tgccgtcaca ctgccccagc
     2221 aacgcttcaa tgccggttaa ctgactatat tcacattgca aagtatattc ggttaatggt
     2281 gtcttgcgtt gggtcgttag ctggcgcagc gcctgattca cgccgccgcc atacgctttc
     2341 actaacccac cggtgccaag caatatgccg ccgtagtagc gcaccaccac agcggtaatt
     2401 tccccgacgc cgctgcccat tagttgggcg agcatcggtt tacctgccgt tcccgccggc
     2461 tccccgtcgt cagagaaacc cagctgttga gaatcatccg gcgcacccgc cacccacgcc
     2521 acgcaatggt ggcgggcatc ggggtgctct gcccgcaccg attcaacaaa cgcttttgcc
     2581 gcctcaacgc catcggtatg cgccaacagc gtaatgaagc ggctcttttt gatctcttca
     2641 acgaccgtga ccggtgccgc aggaattaac cagctttcca tcacgccagt ttcagatccc
     2701 gggtaatgtt ttccacgttg ttttcgtgaa tcaccacgtt gtcttcgata cgaatgccgc
     2761 cgaacggttt cagtgcttca attttctgcc agttgaagtg tttgctgaac tgcccttcac
     2821 gccacggtgc cagaagcgat tcgatgaagt agatacccgg ttcgatggtt aacaccatgc
     2881 ccggctggag aatacgggtg cagcgcaggt acggatattt tgccggtgcc gcgaggtgcg
     2941 taccgctatc atcctgcata aaaccggcta cgtcatgcac ctgcaggccc agcggatggc
     3001 cgataccgtg cggcataaac ggtccggtga gatcgttttc gaccatcgct tcttcactca
     3061 tatcggtgat gatttgatgt ttacgcagca gtttggcgat gcgctgatgg aactggatat
     3121 ggtaatccac atagctgacg cctgctttca tggtagcgat cagcgccagt tgttcatcat
     3181 ttacgtcttt caccagctgt gcgtagtcgt tgtcgctttt tgccgaccag gtacgggtta
     3241 gatcagccgc atagccgtta tattcggccc cggcatccag caggaagctg cgcatctctt
     3301 ccggtgcctg gtggtccagt ttggtgtaat gcaacaccgc agcgtgttcg ttaagcgcca
     3361 caatgttgct ataaggtacg tcggtatcac gatgaccggt cgcggtcaga taggcaatgt
     3421 tgatatcgaa ctcgctcatg ccagaacgga acgcttcttc tgccgcgcga tgaccgttga
     3481 ccgccatttt ctgcgcttca cgcatacagg ccagttcgta ctcggttttg aaggagcgat
     3541 agtaatgcag gtagtcgatc acccctttcg ggttgatatt gctggcctca atacccagtt
     3601 gcagcgcacg ttctggcacc ggaccgatat aaccgatatt gccgcgcgca gcaggcaaca
     3661 gactaccaat gccatcggct ttcggcagcg cgatcacttc tacatcttca gtccagaagg
     3721 agttcggcag cggttcgacg ttgtgccagt aatcaaccgg tagatagaac cacagtttcg
     3781 gcttattcac gccatccacc agcagccagc agtttggcac ctgagttact ggcacccacg
     3841 ctttgaattg cgggttcact ttaaacggat agggatgatc gtcgagaaaa acgttgaaca
     3901 gttcgccgga gtgaataagt aacgcatcca gcttgaagcg cgccagcgca tcgcgagtcc
     3961 gttcctgtaa ggtagctata tgatttttat agagcgaggc cagtgattcc attttttacc
     4021 cttctgtttt tttgacctta agtctccgca tcttagcaca tcgttcatcc agagcgtgat
     4081 ttctgccgag cgtgatcaga tcggcatttc tttaatcttt tatttgcata tttttaacac
     4141 aaaatacaca cttcgatgca tctggtacga ccagatcacc ttgcggattc aggagactga
     4201 catgctttac aaaggcgaca ccctgtacct tgactggctg gaagatggca ttgccgaact
     4261 ggtatttgat gccccaggtt cagttaataa actcgacact gccaccgtcg ccagcctcgg
     4321 cgaggccatc ggcgtgctgg aacagcaatc agatctaaaa gggctgctgc tgcgttcgaa
     4381 caaagcagcc tttatcgtcg gtgctgatat caccgaattt ttgtccctgt tcctcgttcc
     4441 tgaagaacag ttaagtcagt ggctgcactt tgccaatagc gtgtttaatc gcctggaaga
     4501 tctcccggtg ccgaccattg ctgccgtcaa cggctatgcg ctgggcggtg gctgcgaatg
     4561 cgtgctggcg accgattatc gtctggcgac gccggatctg cgcatcggtc tgccggaaac
     4621 caaactgggc atcatgccgg gctttggcgg ttctgtacgt atgccacgta tgctgggcgc
     4681 tgacagtgcg ctggaaatca ttgccgccgg taaagatgtc ggcgcggatc aggcgctgaa
     4741 aatcggtctg gtggatggtg tagtcaaagc agaaaaactg gttgaagggg cgatggcgat
     4801 tttacgccag gccattaacg gcgacctcga ctggaaagcg aaacgtcagc cgaagctgga
     4861 accgctaaaa ctgagcaaga ttgaagccac catgagtttc accatcgcta aagggatggt
     4921 cgcacaaacg gcggggaaac attatccggc ccccatcacc gcagtaaaaa ccattgaagc
     4981 ggcggcccgt tttggtcgtg aagaagcatt aaatctggaa aacaaaagtt ttgtcccgct
     5041 ggcgcatacc aacgaagctc gcgcactggt cggcattttc cttaacgatc aatatgtaaa
     5101 aggcaaagcg aagaaactca ccaaagacgt tgaaaccccg aaacaggccg ctgtgctggg
     5161 cgcaggcatt atgggtggcg gcatcgctta ccagtctgcg tggaaaggcg taccggttgt
     5221 catgaaagat atcaacgaca agtcgttaac cctcggcatg accgaagccg cgaaactgct
     5281 gaacaagcag cttgagcgcg gcaagatcga tggtctgaaa ctggctggcg tgatctccac
     5341 aatccaccca acgctcgatt actctggttt tgaccgcgtg gatgttgtgg tagaagcggt
     5401 tgtcgaaaac ccgaaagtga aaaaagccgt actggcagaa accgagcaga aagtacgccc
     5461 ggataccgtg ctggcgtcta acacttcaac cattcctatc agcgaactgg ccaacgcgct
     5521 ggaacgtccg gaaaacttct gcgggatgca cttctttaac ccggtgcacc ggatgccgtt
     5581 ggtggaaatt attcgcggcg agaaaagctc cgacgaaacc atcgcgaaag tcgtcgcatg
     5641 ggcgagcaag atgggcaaga cgccgatcgt ggttaacgac tgccccggct tcttcgtcaa
     5701 ccgtgtactg ttcccctatt tcgccggttt cagccagttg ctgcgcgacg gcgcggattt
     5761 ccgcaagatc gacaaagtga tggaaaaaca gtttggctgg ccgatgggtc cggcatatct
     5821 gctggatgtt gtgggcattg acaccgcgca tcacgcccag gctgtcatgg cagcaggctt
     5881 cccgcagcgg atgcagaaag attatcgcga tgccatcgac gcgctgttta atgccaaccg
     5941 ctttggtcag aagaacggcc tcggtttctg gcgttataaa gaagacagca aaggtaagcc
     6001 gaagaaagaa gaagatgccg tcgttgacga cctgctggca gaagtgagcc agccgaagcg
     6061 cgatttcagc gaagaagaga ttatcgcccg catgatgatc ccgatggtca acgaagtggt
     6121 gcgctgtctg gaagaaggca ttatcgccac tccggcagaa gcggatatgg cgctggtcta
     6181 cggcctgggc ttccctccgt ttcacggcgg cgcgttccgc tggctggaca ccctcggtag
     6241 cgcaaaatac ctcgatatgg aacagcaata tcagcatctc ggcccgctgt atgaagtgcc
     6301 ggaaggtctg cgtaataaag cgcgtcataa cgaaccgtac tatcccccgg ttgagccagc
     6361 ccgtccggtt ggcgacctga aaatggctta aggagtcaca atggaacagg ttgtcattgt
     6421 cgatgcaatt cgcaccccga tgggccgttc gaagggcggt gcttttcgtc acgtgcgtgc
     6481 ggaagatctc tccgctcatt taatgcgtag cctgctggcg cgtaacccgg cgctggaagc
     6541 ggcggccctc gacgatattt actggggttg tgtacagcag acgctggagc agggttttaa
     6601 tatcgcccgt aacgcggcgc tgttggcaga agtgccgcac tctgtcccgg cggttactgt
     6661 caatcgcttg tgtggttcat ccatgcaggc actgcatgac gcagcacgaa tgatcatgat
     6721 cggcgatgcg cagacatgtc tggttggcgg cgtggagcat atgggccatg tgccaatgag
     6781 tcacggcgtc gattttcacc ccggccttag ccgtaatgtc gccaaagcgg cgggcatgat
     6841 gggcttaacg gcagaaatgc tggcgcgtat gcacggtatc agccgtgaaa tgcaggatgc
     6901 ctttgccgcg cggtcacacg cccgcgcctg ggccgccacg cagtcgggtg catttaaaaa
     6961 tgaaattatc ccgaccggtg gtcacgatgc cgacggcgtc ctgacgcagt ttaattacga
     7021 cgaagtgatt cgcccggaaa ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga
     7081 tcccgtcagc ggtacggtaa cggcgggtac atcttctgcg ctttccgatg gcgcagctgc
     7141 catgctggtg atgagtgaaa gccgcgccca tgaattaggt cttaagccgc gcgcccgtgt
     7201 gcgttcgatg gcggtcgttg gttgtgatcc gtcgattatg ggttacggtc cggttccggc
     7261 ctcaaaattg gcgctgaaaa aagcggggct ttctgtcagt gatatcggcg tgtttgaaat
     7321 gaatgaagcc tttgccgcgc agatcctgcc atgtattaaa gatctgggac tgattgagca
     7381 gattgacgag aagatcaacc tcaacggtgg cgcgatcgcg ctgggtcatc cgttgggctg
     7441 ttccggtgcg cgtatcagca gcacgctgct aaatctcatg gagcgcaagg atgcccagtt
     7501 tggtcttgcg acgatgtgta tcggtctggg tcagggtatt gcgacggtgt ttgagcggat
     7561 ttaaccactt gccagatgcg gcgtgaacgc cttatccggt ctaccgatcc tgcaccaatt
     7621 gtaggcctga taagacgcgc cagcgtcgca tcaggcagcg tgtgccgaat gccggatgcg
     7681 acatagatct aaatgccact gtataaagtt ttagttgccg ttcttcccgc ctgtcagggg
     7741 cgggtttttt atatctcaga taaatgcaaa cgcatcgcca aacaggcgat cttcccgcgc
     7801 attacgctcg ctgcaaaaca ggtcacgggc aattttcgcc atctcaaaac gtccggcaat
     7861 atagatatca tgctctgcca gcgtaccatg atcctgcaat accgccgtta acacggtgcc
     7921 agtacgccca cgccagcccg cttccggttg ttcaaccacc ggcaccactt gtagaccagg
     7981 atgcttcaac gaaagcgcct caagctcgca gagatcatac agatgctgct cttcacgccc
     8041 gccccagtaa atggtgatat cacggtttgg gttacgcgcc aacgctgtca gcaaaatcga
     8101 gcgggcataa gagaacccgg tgccgcccgc aatcaaaatc atcggacgct cttcatcatc
     8161 gcgcagccac gcttctccgt ggggaatatc gaccacgatt tgatgatctt tgaggatgcg
     8221 gtccatgact gctttcgcgt aaaggttgat ttcagaagcg ccaatatgca gctcgataaa
     8281 ccctttttca tctggcgtcg aagccattga gaacggacgt ttgtcgcgct catccatcac
     8341 taccatcaaa tactgaccag cacgaaaaga aaaggccgcg tctggcacga tgcggacacg
     8401 atatacggta tccgtgatag cttctaccga ggtcacttta cagcttaagg ttgtcatgcg
     8461 ctttctctgt cggatcgata aatagggcaa aacaaacgcg catcaggcgc ttttaccgtt
     8521 gttaaaaata gccagttcat cccaaatggc gtcaatatgc gcgacaacat ctggatcttt
     8581 tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg tggcatccag
     8641 ccccattttt gaacccagcc cggagacagg ggaggcaaaa tccagataat caataggcgt
     8701 attttctacc agaacggtat cccgcgccgg gtccatacgg gtggtaatcg cccaaatcac
     8761 atcgttccag tcgcgggcgt tgacgtcatc atcgcaaacg atcacaaatt tagtgtacat
     8821 aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc cggcatactg
     8881 ttttttgatc gtcactaccg ccagacgata agaacagcct tccggcggca gataaaaatc
     8941 gacaatttcc gggaactgtt tttgcagaat cggcacgaac acttcgttca gtgctacacc
     9001 cagtaccgca ggttcatctg gcggacgccc ggtataggtg gaatgataaa tcgcatcttc
     9061 acgctgggta atatgcgtca cggtaaatac cggaaaacta tcgacttcgt tatagtaacc
     9121 ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat acccttccag
     9181 cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca ccacttcggt
     9241 cttggtgccg cgcagcaatc cggcaaacgc atactctgaa agcgtatccg gaacgggggt
     9301 gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg ggaaacgttc
     9361 gcccggatgc gccgcacacc actcctgata atccaacgcg ccgccgcgat gcgacagcca
     9421 gcgcataatc agtttgtttt taccaattag ctgctggcga taaatgccca gattctgccg
     9481 ctctttatgc gggccgcgcg ttacggtgag cccccaggta atcagcggcg cggcatcttc
     9541 cggccagcag gtcataatgg gaatacgatt gagatcgacg tcatcgccag agacgatttt
     9601 ttgttggcaa ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt gcttaaactg
     9661 cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tccggctctt tcagaaacgc
     9721 caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc ccatcgccac
     9781 gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt tagggttttc
     9841 gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag tgatttccag
     9901 gtgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa gcagcgtcaa
     9961 gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat ctgcgcccat
    10021 tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa ctctgacggt
    10081 ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc cttgcgcccc
    10141 gattaaacgg ataagagtca ttatgcaatc ctggtattta ctgtactgca agcgcgggca
    10201 acttcaacgt gcccaggaac acctcgaaag acaggctgtg aattgcctgg caccgatgat
    10261 caccctggaa aaaatcgtgc gtggcaaacg tactgcagtc agtgagccat tgttccccaa
    10321 ctacttgttt gtggagttcg atccggaagt gattcatacc acgactatca acgcgacccg
    10381 cggtgtcagc cacttcgtgc gctttggcgc gtcgccagcg atagtcccat cggcggtgat
    10441 tcatcagtta tcagtgtata aaccgaaaga tattgtcgat ccatcgacgc cgtatcctgg
    10501 tgataaagtg attattactg aaggcgcatt cgaaggtttt caggccattt tcaccgagcc
    10561 agacggcgag gctcgctcca tgctattgct gaatcttatt aataaagaga ttaagcacag
    10621 tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaacagtg ttttgacatt
    10681 agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg caatacgttg
    10741 caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg gcgtgagatc
    10801 gcgagggagc agatacggcg catcagtttc gatcagcaat ttctccgccg gaatcaacgg
    10861 caacaattcc cgcagctcca gcccgcgtcg ttcatcgcaa acccaaccgg taatgccgat
    10921 ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa agcaatgaag
    10981 aaccgcacca ggcactttat ccagccacgg ctccagcaat gtcataaacc gctcgtgggc
    11041 atcgcgacag tgcataaata ccggcatgtt taattctgcg gcaatgcgta gctgggcaac
    11101 aaaagcgcgt tcctgttctt ccggcgtcga aaagttgcgg ttaaagtcga gaccacattc
    11161 accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt cagtcgcagc
    11221 ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg acgaatactg
    11281 acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga tgagtagccc
    11341 attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg caaattgcga
    11401 actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac cgccctgacg
    11461 ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt ttcctcttcc
    11521 cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc aaacaggcaa
    11581 tacatcggga tcgccagcag cgtttgcgag aagacatccg gcggcgtcag cagcatcccg
    11641 acaacaaatg cgccaaccag cacatacgga cgttttttgc gtaagtcttc tggcgaggta
    11701 attcccatcc agcacagcag cactattgcc accggcactt caaaggagac accaaacgcc
    11761 ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac ctgtacccct
    11821 tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa gtaggcgaac
    11881 gccatgccga tataaaacaa cagagagctg gaaaccagca gcggcaccac caggcgacgt
    11941 tcatgcttat acagcgctgg ggcgataaac gcccacacct gatagagaat caccggcgct
    12001 gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg cgaggccacg
    12061 tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc ggataccagg
    12121 tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac cgcgataatg
    12181 cagttcagca gacgcttacg cagctcaatc agatgcgtga taagcggttg agtatcttct
    12241 acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt ttcggttcag
    12301 cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt tccggcgaac
    12361 tggcttgcgt ttgtgcagca gcaggcgtta cgccttcatg cgcagcttca ttgtctttca
    12421 ccaccgggtt gtggatggtg tgcgcttcat cgctcgcctt ttcaggatcg tttgcaacgt
    12481 aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct ttcagttcgg
    12541 gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga aactcctgga
    12601 gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc aacgcgcgaa
    12661 tccagcccgc gaccgttttt accgccacag gcagtcgttg cggccccaga acgacgaggc
    12721 cgatgatgaa taccaatagc agttcgctaa aaccgatatc aaacacggat tacacctgct
    12781 ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta tccgcctgct
    12841 tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc tgctttggtt
    12901 catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg gaaccgatgg
    12961 agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc aataactgcc
    13021 aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa caaggccgca
    13081 ttatacgtta cacggcccgc cttgagcgat gaaaaaatca gcgtgttttg cgccaaccga
    13141 caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc cattcaggtc
    13201 ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg agaaaataac
    13261 gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta tcaacactgt
    13321 gctgtaaata cttgccctgg cgcaaactgt cgtaaaccag ttcaggcagt tctggcattt
    13381 tttcgaccca gaacggcgct ttttctttaa atgctctcac cagcgcagga ataccgacct
    13441 gatctttaat ccacgactca aggaaaggct tcgccgtttt ccataaatcg agctgcggat
    13501 aaagctggcg tcctacccct tcgacgtaga gcagggtttt ctggagtaac accagttgcg
    13561 gctgcacttc catattgaag cgacgcgccg tattaaatag atttaacagc acatgtccaa
    13621 acgaaatttc ggccaacggt ttctcaaaga taggttcaca gaccgtacga atggcaaatt
    13681 cgaactcttc aacgttggta tctggcggca cccagcctga atcgacgtgt agctctgcaa
    13741 ctttgcgata atcgcgatta aagaaagcga taaagttttc cgccagatag cgtttatctt
    13801 ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg ttttctgggt
    13861 gttcatagct tacaaagatg ttgccagggt gcatatcggc atggaaaaag ctgtcgcgaa
    13921 agacctgagt gaagaacacc tgcacgccgc gttccgccag caatttcatg ttggtgccgt
    13981 ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatacgctcc atcaccatca
    14041 tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg ctgtcttcaa
    14101 aattgcgtcg aagctgaatg gcgttggcag attcccgcag caaattcagt tcatcaatca
    14161 aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca tccggcagca
    14221 aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta atgaccggca
    14281 aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc aatcgcgcgg
    14341 tatgaacctg ggcgatagaa gcagaagcca gcggcttgat ttcaaaatcg tcaaaccacg
    14401 cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc ttgccatcaa
    14461 acggagcgac tttgtcctgc aataacgcca gctgatcggc aatatgcggc ggaaaaagat
    14521 cgcggcgggt tgataacatc tgcccgaact tgatccatac cggtcccagt tcttgcaggg
    14581 ccagtcgtag tcgctcacct aaaggtttgt ctttatgccg atttggcatc cagaataatg
    14641 agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt tcatcaagtc
    14701 cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct ggcgtcattt
    14761 agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga cggcagccgt
    14821 ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact cttcagtaat
    14881 ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact ttgcgccccc
    14941 gcgcagggct ttgctgatac cttcagcggc gatatcaccg gtataagggg ccagcagttc
    15001 cgcagggtcg aactcagcca gatctgccag cgcaacgaag ttttgcacca cctgaatatc
    15061 gccctgcact tccagctcac cactgcgaat cagtgcggca agctgctggc gatcgcgaag
    15121 tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg cccattcgcc
    15181 cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa agccttttac
    15241 ctccacgcgc agcactttgc ccaacagacg cgagcgggcc gttttcagcg cgggtgagcg
    15301 atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt taaaaggcat
    15361 ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct gccgtcagat
    15421 tgtagtagtc gacactttcg aatccggcat cctgcatcat ggttttcagg gtatcctgat
    15481 cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg ttcgcgacca
    15541 gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg ctcagcggct
    15601 cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc agcacgcgat
    15661 acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac gaaatggtga
    15721 tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga acatactcaa
    15781 cgttgccaat cacaccgata ttacgcagct tctcgcggcc cattttgagc atggattcat
    15841 tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgagagaat ttcgctgtca
    15901 ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg ccgctgcaat
    15961 caatcgtgaa tcgcttccac aaacgatgaa taccgaatga catcaaatca ttcatgacat
    16021 cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt tgttccttcg
    16081 cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc tcaatgcctg
    16141 ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct agatttctac
    16201 ccggcttaac tacccccaat gggctagcgc gactgctgat tatattcatc atcgcgttga
    16261 taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc atcctggctc
    16321 accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctacccc taaaccgcga
    16381 aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt cattgcctgg
    16441 cggtagttat cctgcgcttt gtcgagactt tgaccaatcg ctgacatgtc atcgacgaac
    16501 aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc gttgcggctt
    16561 tgatgctcat aacgccacag gttggcgata gtgcgcagcg ccaccagcag cgtagtcggg
    16621 ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg gcggtcaagc
    16681 gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag ggttcgcagc
    16741 cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg caccgacgcg
    16801 atatgttcct gtagcgcgct ttcgcgggtg tagtcatctt cggcgttgaa atagcgttca
    16861 taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg caaccgcacg
    16921 atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg agtttcatat
    16981 tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaataccac ctcgccccag
    17041 ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc ctcctgagcc
    17101 atttgcgcgt tgagctgctg gagattgcga atttcgtggg tcagggtatg acgttcctgt
    17161 gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg ttcacgtagc
    17221 ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg attgctgtgc
    17281 tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc gctgttgatc
    17341 atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt ggttacttca
    17401 cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc gttattgagt
    17461 aactcgcact cagcgcgcca gtgctcgctt tgagtaattt gttgttttgc cgcgcttaac
    17521 tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc atgttgataa
    17581 ctggcaaaca gccagccaat gaccacacta accagcgcaa taatggcata aacaatgatt
    17641 gagaaatcca caaagcctcc tgacccaaca acttacccgc acaaaataga attgtgctgt
    17701 acaaacgtcc agtttgaaag aagtttcctg cgaggcgcta cgcaaaacga tcgttaattt
    17761 tcattatttt atcccacaat atgagatgga gtttatattg tgggatatat caccttttcc
    17821 ccacgtcatt atgtggaaca cttcagcaat atcatcagga gaaacgtatg gcagaaacct
    17881 cttcaaccac cgcaggcgca caaacgttat tacgtggact ggcggttctt aatgctgttt
    17941 ataacggctg ccatgaccta aaaagtatcg gcgagttcac cggcacgacc cggagcacaa
    18001 cccatcgttt agtcaccgtt ctggtggaac aacgctacct gcgccatgtc ccaacgcagg
    18061 gctaccaact gggtgccaaa ctgatcgaat tcggtgccag agcactggaa agcacctcac
    18121 tgtacgaaat tgcccagccc gttttgcaac gccttgcccg ttacaccctg gacaccgtgc
    18181 atctggggat cgttgaaggc gacgaagtgc tgtatctgga gaagatcaac agtcagcgcg
    18241 ggctggaaat gcgctcacgc ccaggccatc gcatgccact ggcaatcacc ggcatcggca
    18301 aggcattgat cctcaaccga acagaagaag agtggcgcac gctgtttaag acctgtggtg
    18361 atgaaacaaa actgggcgca tttattcaga acatgcgtcg ttatgccgcc agtggttttg
    18421 cttttgatct ggaagagaac gagccgacta tccgttgcgt tgcagcgccg gtttacaacg
    18481 cccgggatga aattgtggcg gctatttctg tcgccagtac caccacgtat atgtcgctgg
    18541 cgcgtctgga agaactggca ccttacgtca aatcctgtgc agaagagata tccgctgaac
    18601 tgggctgggg taagcacgtc cgcaaagata aataaaccat gattaaagga aacgactaat
    18661 gccgggagca ccctcattta ttgccctgga ctggggaacg tcatcgcttc gggcatggcg
    18721 ttttggcgaa tcaccaaatc cacaagaaaa acgtgaattc ccctggggga tcatgaaatt
    18781 accctcccag gcagcaacac gtgaggacgc tttccacgat acgtttttac gtgtctgcgg
    18841 cgactggctg gcacaaaccc catgcccggt actggcttgc ggcatgctcg gcagtgcgca
    18901 agggtggcaa tcagcagctt atttaccttg cccggtcact ctcgagggtc tggcgaagca
    18961 gttgacgcct gttatccacc agcagcagac aatgctgcac atcattcctg gcgtgattaa
    19021 agaaggtgaa atgcccgaag tgatgcgtgg cgaagagaca caaatcttcg gtgctatctc
    19081 gatggaaccg gccctgcaaa acgcgatcca tcagggtatg ccagtgctga taggcttacc
    19141 cggcacacat gcgaaatggg cagtagttga aaacaacacc attaccgatt tccgaacctt
    19201 tatgacgggc gagttatttg atgttttatc ccgccattcg attctcggtg ccaccatgca
    19261 tcctggagat gaaccgcact gggatgcctt cacccacggg ctgacagcag cacaagagca
    19321 tcatcagacc ggattattat cgacgctgtt ttcgacccgt tcgcgcctgc tgaccagtaa
    19381 tcttacgtcg tcctcgcagg gggattacct ttccggatta ctgataggcc atgaattatg
    19441 tggtctggca tccagtttgc tacgtgattt acccgccaca acaccgatcg ccttaatcgg
    19501 tagcgcaaat ctgaacagcc gctattcaca agcattcagc cacgtttttc ccgacagaca
    19561 gatacacgcc attcctaatg ccactgaaca tggattatgg cgaatcgccc acgctgccgg
    19621 gttactgtcc accaacgcca gggaatgtac ccatgccatt taatacactt ttgcaaaaaa
    19681 ccgggttagt cgccatttta cgcggcgtga aaccggatga gattgtcgcc atcggtgaaa
    19741 aactgtacgc cgccggattc cgcctgattg agattcctat gaactcgccg gaggcgttgc
    19801 aaagtatcag tttactgcgc gacgccctgc ctaaggactg cctggtcgga gccggaactg
    19861 tgctttcggt agagcaggtg gttgcggtta aagaggctgg gggccagatt atcgtgatgc
    19921 cgcattgcga tactgcggtg atccgtcgcg cccgtgcgct gggtatgtat tgtgcgcctg
    19981 gcgtcgccac gcccaccgaa gcctttgctg ccattgaaca tggtgctaat gccatcaagc
    20041 tgttcccggc tgaacaaatt acgccagaag ttaccaaagc ctggcgtgcg gttattccac
    20101 aatccgttcc gatgttaccg gttggcggta tcacaccgga aaccatggcg cgttatctga
    20161 gccacggcgc aaacggcttt ggcctcggtt ctgcgctata tcgccccggc atgacgccag
    20221 agcaagtcta tgaaaatgca gtactgttta tgaacgcatg gaacaattta aatagcaact
    20281 aactgataaa taaggatata aaagcaatga taaaacatct taccttaccg aaaaaaatgg
    20341 ctgcaatggt acttcttgca ggcgctgccc tcagcattgc gcctgtacag gctgcctcct
    20401 acccaaccaa acagatcgag ttagtcgttc cctacgctgc cggaggcggt acggatctgg
    20461 ttgcccgtgc ctttgctgat gccgccaaaa accatttacc cgtcagcatc ggggttatca
    20521 ataaacctgg cggaggcggt gctatcggcc tgagtgaaat cgccgctgcc cgccctaacg
    20581 gttacaaaat tggtttaggc acggttgaac tgaccaccct tcccagcctc ggaatggtgc
    20641 gttttaaaac cagcgacttt aaacccattg cccgtctgaa tgcggatccg gctgctatca
    20701 cagtccgtgc cgatgcgccg tggaatagct atgaagaatt tatggcttac tccaaagcga
    20761 atcccggaaa agtacgcatt ggtaactcag gcaccggagc tatctggcat ctggcggtag
    20821 ctgcactgga agacaaaacg ggcacaaagt tttctcatgt cccgtatgac ggcgcagccc
    20881 ctgccattac aggcctgtta ggcgggcata ttgaagcggt ttccgtaagc ccaggagaag
    20941 ttatcaacca tgtgaatggc ggcaagctga agacactggt agtgatggcg gatgagcgaa
    21001 tgaaaaccat gcctgacgtc ccgacgttaa aagagaaagg cgttgatctc tccatcggca
    21061 cctggcgcgg cctgattgtg tcgcaaaaaa cgccgcagga tgtggtggat gttctggcaa
    21121 aggcagcaaa agagacggct gaagagcctg cattccagga tgcactgcaa aagttgaatc
    21181 tcaactatgc atggcttgac gctgccagct tccagaccca aatcagcgaa caggaaaagt
    21241 actttgacga gttgctgact cgcctgggcc tgaaaaaata aacctgaggt acggttgatg
    21301 aacacaaaac agtctgttgc tcaactcgcc gtaccgcacc gcaagcgcct ttcatcaacg
    21361 atggtggtgg cgctgttact ttgtgtggtt gctggcgcgg tgatgattaa tgccgctgat
    21421 tttccagcaa ctgccattga aacggatccc ggtgcaagtg ccttccctac cttctatgcc
    21481 tgtgccctga ttgtgctcgc tgtcttgctg gtgatacgcg atcttttgca ggcaaaacca
    21541 gcctcttgcg ccaacgcaca ggaaaaaccg gcattcagga aaacagcaac aggaattgcg
    21601 gcaaccgcgt tttatattgt ggcgatgagc tactgcggtt atctcattac tactcctgtt
    21661 ttcctcatcg tcattatgac gttgatgggc tacaggcgat gggtactcac accgggtatt
    21721 gcgctgctgt taacggcaat cctctggttg ctgtttgtcg aagcgttaca ggtgccattg
    21781 cctgtcggca catttttcga ataacagggg ccagcaatgt ttgaaagtga acttttaact
    21841 cagggttttt cgacattact caataatcct caggcgctgc tgtttgccac ttttggggtg
    21901 atgctgggta ttgtgattgg cgctttgcct ggtctgacag cgaccatggg tgtggcgatt
    21961 ttgctgcctt tcacctacgg catggagcct gtttctggct tgttgatgat ttgcggcgtc
    22021 ttttttggtg gcgtctacgg tggttctatc accgcaattt tgctcaaaat tcccggaacg
    22081 cctgccgccg cagccaccgc tattgatggt tatgagctga cgaaacaggg aaaagcaggg
    22141 ctggcattga gtgccgccac gttctcttcc tttagtggcg gaacgctcag cattatcgtg
    22201 ctgatgtttc tctctccggt acttgccagt tgggcgctga aatttagtgc ctcggagtcc
    22261 ttcgccctgg caaccttcgg actgagtatt attgccagta tttccggtga gtcgctgatt
    22321 aaagggctga ttgccggggt tggcggattg ctgatcgcaa cgataggcct tgatccaatg
    22381 ggcggttttc cacggtttac aggtggattt gtggagctga tgaatgtgcc atttatcccg
    22441 gtgatgattg gtttatttgc tgcttcagaa gcattccgct ctatggagca aaaccagcaa
    22501 attcgtcagg gggcgaaggt ggctatcggc agtctgttac tgccctggca aacactacgc
    22561 cgcattgcat taaccatttt gcgctcatca ggattagggg tttttatcgg catgatcccc
    22621 ggtgcggggg cggatattgc agcttttgtt gcctataacg aaactcgtcg tttcagtaag
    22681 acaccagaaa actttggtaa gggtgaaatt aaagctgtgg cctcctgtga ggcaggcgcg
    22741 aatggctgca ccggtggcgc attgttacct atgctgacgc tggggatccc tggcgatgcg
    22801 gtaaccgcca tcatgctggg cgcgttaacg ttacagggaa tgcaaccagg cccgctgatg
    22861 tttaccgacc acggcgatat ggtttataca ctgtttgtcg gcatgatctt ttgttacttc
    22921 atgctgctag ttcttggact gctctctttg aaagtcatcg gtaacgtggt gaagatcccc
    22981 ggcaatattc tcacaccgat gatcctcgca ctttgtgtgg tcgggactta tgcgttgaac
    23041 aatagcctgt ttgatgttgg cattatgctg attgcaggcg tggtgggcta tttcatgcag
    23101 aaaggaggat atccggcatc acccgtagtg ctggcattga ttatgggccc gatggcagaa
    23161 agtaattttc gccgtgcgct gtcgctttca gggggttcac tggactttct gtatacccga
    23221 ccgatcactc tggcattgct aactctggca gcctttacgc tactgacgcc aataatccgc
    23281 aaaataatgc gtttacggcg tcagtaactg atatcgctcc ccgaagttgg ggagcgtttt
    23341 ttatttcagc agacgacgcg ccgcttccac cacgattttc accgcatggc tctcggtttg
    23401 tttcatcgtc tcagcattcg ggatctcttg ctgggtgcgg ttaacgataa cccccgctac
    23461 cataccggca cgcaggccct gacttgcaca catggtcagc agggttgcag attccatttc
    23521 atagttcatt acgcccatcg cctgccactc ttccatagaa cctttaaagt gacgaactac
    23581 gcgaccagag taagtatcgt aacgttcctg acccgggtag aaggtatcag aagaagccgt
    23641 cacaccaacg tgagtggtgg caccaattga cttcgccgct tcaaccagcg cagtcgtaca
    23701 ttcgaaatca gcgacagccg ggaattccag cggtgcgaag tgcaggctcg cgccgtccag
    23761 acgaacagac gccgtggtaa ccaggacatc acccacatta atatgcggct gaatagcgcc
    23821 cgttgtaccg atacgcagga aggtgcgaat gcccagctgt gccagctctt caacagcaat
    23881 agaggtagac gggccgccga taccggtaga gcagacgata acaggtttac catccagctc
    23941 tgcacgccag gtggtgaatt cgcggtgaga tgccagctta accggcttat ccatcagcgc
    24001 ggcgatcttt tccacacgat ccgggtcgcc agggacgatg gcaagcgtag ccccttgtaa
    24061 atcgttttta gtgaggccga gatgaaaaac atcagacttg gacatataca actcctctgt
    24121 gaatcggttt agtcagaaga agcaaaaaga cactttaccg aagggtttaa cattttttcg
    24181 tgatactcat caccatgacg caaatgcgtt gcataaattt acccatcaaa agtgatgcaa
    24241 atcacataaa tcactcttca ttctgcagga ctgcataaaa gatgagccga ttcaggcaat
    24301 gtgatttgtt gcatgctgac tatagttaac attgcgcaac atttttataa gggtacggag
    24361 aataccatgg caacaacaca acaatctgga tttgcacctg cggcatcgcc tctcgcttcg
    24421 accatcgttc agactccgga cgacgcgatt gtggcgggct tcacctctat cccttcacaa
    24481 ggggataaca tgcctgctta ccatgccaga ccaaagcaaa gcgatggccc actgccagtg
    24541 gtcattgtag tgcaggaaat ttttggcgtg catgaacata tccgcgacat ttgtcgccgt
    24601 ctggcgctgg aggggtatct ggctattgca cctgaacttt acttccgcga aggcgatccg
    24661 aatgattttg ccgatatccc cacgttgctt agcggtctgg tagcaaaagt gcctgactcg
    24721 caggtgctgg ccgatctcga tcatgtcgcc agttgggctt cccgcaacgg cggcgatgtt
    24781 catcgtttaa tgatcaccgg attctgctgg ggtggacgta tcacctggct gtatgccgcg
    24841 cataatccac agctaaaagc cgcagtggcg tggtacggca aactgacggg cgacaagtcg
    24901 ctgaattcac cgaaacaacc tgttgatatc gcaaccgatc ttaacgcgcc ggttctcggc
    24961 ttatatggtg gtcaggataa cagcattccg caggagagcg ttgaaaccat gcgccaggcg
    25021 ctgcgggctg ctaatgcgaa agcagagatt atcgtctacc cggatgccgg gcatgcattc
    25081 aacgccgatt atcgcccgag ctatcatgcc gaatctgcga aagatggctg gcagcgtatg
    25141 ctggagtggt ttaagcagta tggtgggaag cggaattagt ttcctcttag tctatatttg
    25201 agatggtgag tgaacgagtg cattttgctg ccatctctgt cccccaaaaa ataatcagta
    25261 tgaattgatt ggagagcacg actccgctat ttgaccttaa gaaagtcggc aagtcatagt
    25321 cataaatgag tctcttcact ggcagacacc ttagagattt accatcaact cccattctca
    25381 aacaaacatt aattattatc aacacattca cattaattat aaattatctc agaccttcct
    25441 gataaaaaga tattaaagtg aaacattttg tttttcacat tttagttcat cccttttggg
    25501 cgtaaataaa ggtaaaaaat aacaatacta tttttctttc acaaaagaat taacaaaagg
    25561 tttttcaagg atgagtgata ttatttacct gaccataacc ggagaacaac aaggatgtat
    25621 ttcctctcgt tgcggaacga gcgcatcaat aggtaatcgc tggcagattg gccatgaaga
    25681 tgaaatcttc gcgttctcgc tatctaatag tattacgaat acaggcaaag ggtcacaatt
    25741 acatggcctt agtttctgta agttgattga caaaagctcc ccgttactta ttaacgctat
    25801 taacaataac gagcagttgt tcatggagtt tgacttctat cggatcaata gatttggtcg
    25861 atgggagaaa tattacaaca ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct
    25921 gtttactgag aacaatctgg atacagaaac catcactgtc agttatgaat atatactctc
    25981 caggcatctg attgctaata ctgaatttag tcatttggca tttcccgata actacaaccg
    26041 tctgtttata ccacggcaaa aaactaaaga tgataatggc ctcaaaacac tcaatagtaa
    26101 agcggttggc agactgcttg ctgctggcgg catatacaat gggaatatcg aaggattcag
    26161 ggaaacagca aacaagttgg gaggagatgc acctgctggt tacgatcaag ttatggataa
    26221 caaagggtta ctcatcgctg gtgcttcaat agttgctgga gttgttctgg gaagaatgag
    26281 attccctgat ttggaatcat ttgagcattt cggagccaga ggagcagtat caggaagacc
    26341 atttaagccc gaccttgccg gtggtccaat agaaaacctg acgatagatg gcgtaacaat
    26401 caatagggaa ggtatcgcta ttgttgaaaa gcatatctcc agatttgatc atgatccagc
    26461 taatgatgta atgatttcaa ggctaaaaaa aatagcaaat aaagaattgc ttcctgaaaa
    26521 atacgatctg aactactata ctcatgaatg ccgcgagtat cagcgttatt gtaatttagg
    26581 atgggaaaca ggtgaaccga atggtttaga tggatatgaa ttatggaata atgcacatac
    26641 tgcaactcta gaagacttta aaattaaaga tactgactta tttcatccgg atgcaaaaaa
    26701 atgacgatta cgatagttga atttaacgtg ctgaaagtta tggctgagaa agatattgac
    26761 tggagctgga tggtgctgga tcgtacttta gctatacgaa atattccagg atttggcaac
    26821 gtagcgaata tcgtaacaaa gctggttaat catgggttgg ttgatatcgt aaatggtgag
    26881 ggcaactcta aaccacgcta cagagtttct caatatggat tgaatcttat caaagaacaa
    26941 caagacaacc tcttctgatc cagccattac aggagcatga tttacattgt gatctttcaa
    27001 taagatactc atcaattttg gtacagaata tcgtgatcac taaaaatgaa cttaacgtgc
    27061 tgaacgttat gactgagaaa gatataaact ggaactggat gaatctcgat cgcacactat
    27121 ccataaggca aataccagga ttcggtaatg ttgttaatat tgttaacaaa ttagctaacg
    27181 aaggtttggt tattattgag gatagcgggc ataagtctat gcctcactac cacgttagtg
    27241 aaaagggata caatctcgtg caacgtgaga ataaataacg tagcgttgat tactgaacgg
    27301 aatcactcaa aactgtgttt tgcgaaaaaa acgattcggt tatttctact cctgaagatc
    27361 tgcttcaatc tggcactcat gcaaaatggc ttccagtact ggaagccatt atcgtactgc
    27421 aatttacaat gactcgttaa tactcatcat ccccgacgca aattctgcgc cgcctgcacc
    27481 atgttcgcca gtgccgcgcg ggtttctggc cagccgcgcg ttttcaggcc acagtccggg
    27541 tttacccaca gacgctctgc cggaatgcgt tttgccgctt tcttcagcag ggcttcaatc
    27601 cattccacgc tcggtacgtt tggcgagtga atgtcataga cgcctggacc aatttcattt
    27661 ggataatcaa actcttcaaa cgactccagc aactccatgt cggaacgcga ggtttcgatg
    27721 gtgatgacgt ctgcgtccag cgccgcaatc gaatccatga tgtcgttgaa ctcgcagtaa
    27781 cacatgtgag tgtggatttg tgtgtcatct ttcgccacgg cggcgttgat acggaaagcc
    27841 tccacgcccc actggagata ggcatcccag tcgctgcgac gcagcggtaa accttcgcgc
    27901 agtgccggtt catcaatctg gatgatgcca attccagcgg cttccaggtc cgccacttca
    27961 tcacgcagcg ccagcgcaat ttgtttggcg atggtttcac ggctgacatc ttcacgcggg
    28021 aatgaccagc agagtatggt taccggcccc gtcagcatcc ctttcaccgg tttgtcggtc
    28081 agcgattgtg catatttcgc ccactccacg gtaattggtg ccgggcggct aacgtcacca
    28141 atgacaatcg gtggcttcac gcagcgggaa ccgtagctct gtacccaacc gttttgcgta
    28201 aagacaaagc catccagatg ctcgccaaag tattccacca tgtcattacg ctcggcctcg
    28261 ccatgtacca gcacatccag tcccaaacgt tcctgctcga caatggcctg cttgatatgt
    28321 tccgcaatgc ccgtgcggta gttgttggcg tcgagattac cctttttgaa atccagacgc
    28381 agtgtacgaa tttccgtggt ttgcgggaag gaaccaatcg tggtagtcgg ccacgcgggc
    28441 agtttaaaac gcgcacgctg ggcttcagca cgcacttcat agacattcgc acgctgactg
    28501 tcctgggcgg taatggctgc aagacgcttt tctaccgccg gattatggac acgggtagag
    28561 tgacgacgcg cctgaatcgg agcgctccat tctgccagag ccgccgtatc accactgttc
    28621 agcgcatcgc gcagcagtgc cagttcatgg catttttgta gggcgaaggc aaaccagctt
    28681 ttcacttctg catcaagacg cgtttccacg ctcaggtcga tggggctgtg cagcaacgag
    28741 caggaagatg ccacccacaa atcgcgtttg ccgacaatgt ccttaatttg cgcatatttc
    28801 tcggtaagat cggcgcgcca gacgttacga ccattgatca gacccgcaga cagtagccag
    28861 tcagaaggca gacgcttgtg cagttcagca acgtcatctt taccatgtac aaggtccaca
    28921 tgcagcccct gaacaggcag cgcagtaatc gtgtcgagat ttggcgttac gccttcaaaa
    28981 taggtagtca gcagcagttt cacctggccc tggagcgcgt cgtaagcggg tttgtatgcg
    29041 tccagccacg cctgcggtag ttccagtacc agcgctggtt cgtcgatctg tacccactca
    29101 atgccgcgtt ttgccagttc tgccaacact tgctgataaa ccggcagaat gtcgttcagc
    29161 aggctcaggc gatcaaactg ttcacctttc actttaccca gccacaggta ggtaaccggc
    29221 cccagcagca cgggcttgac cttgtggccc agcgccagcg cctcgtccac ttcctccagc
    29281 agctgcgtcc aggtcagttt gaactgttgg cctttaacga actccggcac catgtagtga
    29341 tagttggtgt taaaccattt ggtcatttcc gctgccgccg caggttcgcc agtcggtgca
    29401 cgtccacgac caatacggaa cagggtgtcg atatctaccg aaccatcttt gttctgatga
    29461 cgtggcggaa cgttacccag cagcagactg gtggtcagta catgatcgta ccaggcaaaa
    29521 tcgcccaccg gcagcaggtc gatacccgcc tgcttttgtt gatcccagtg acgggcacgt
    29581 aattcacgcc caacagccag cagttcttca cgcgtggagt tccccgccca ataactctct
    29641 tgcgcttttt tcagctcgcg acgcaggcca acgcgaggga aaccgagggt gtgattaaga
    29701 attgtcatta tatgcccctt atggaatttt tagtatttag ccgtccagat gtttacacat
    29761 ccataattgg cggttactgt atattcctca agcgcaaatt attcatgccg aagtgaagga
    29821 ctttcatgat cgaagtaaaa cacctgaaaa cgctacaagc gttgcggaac tgcggctcac
    29881 tcgcagccgc tgcggcgacg ttgcatcaaa cgcaatccgc cctgtctcac cagtttagcg
    29941 atctggaaca acgccttggc ttccggctat ttgtgcgtaa aagccagccg cttcggttta
    30001 caccgcaggg agaaatcctg ttgcaactgg cgaatcaggt gctgcctcaa atcagccagg
    30061 cactgcaagc ctgcaatgaa ccgcagcaga cgcgcctgcg cattgccatt gagtgccata
    30121 gctgtattca gtggctgacg cccgcgttag aaaatttcca taagaactgg ccgcaggtgg
    30181 agatggattt taaatcgggc gtgacgtttg acccgcagcc cgcgttgcag cagggtgagc
    30241 tggatctggt gatgacctcc gatattctgc cgcgcagtgg cctgcattat tcgccgatgt
    30301 tcgattatga agtgcgtctg gtgttggcac ctgaccatcc actggcggcg aaaacgcgaa
    30361 tcacgccgga agacctcgcc agcgaaacgc tgttgattta tccggtgcag cgtagccgac
    30421 tggatgtctg gcggcatttt cttcagcccg caggcgttag cccgtcactg aaaagcgtcg
    30481 ataacacctt gttgttgatt cagatggttg ccgcgcggat gggcatcgct gcgctaccgc
    30541 attgggtggt ggagagtttt gaacgccagg gtctggtggt caccaaaacc ctgggcgaag
    30601 gcttgtggag ccgactgtac gccgccgtgc gcgatggcga gcagcgtcag ccgattacag
    30661 aagcgtttat tcgttcagcg cgcaatcacg cctgcgatca tctgccgttt gtgaagagcg
    30721 cggagcgacc cacttacgat gcacccacag tgaggccagg atcaccagcg cgcctgtaat
    30781 aaacgttggc cagtgcggct gttggtgcca gatagccagg ttaaccaaca gccctgccgg
    30841 aacgtgcata ttattcataa tgcccagcgt tccggcgtcc acctgagtcg cgccgtagtt
    30901 ccacataaag tagccaatac cggaagccac cacgccaaga aacaccagaa tgccccattg
    30961 cagcgtggtt tgcggcattt tctgcgcatt tcccagcaaa aaccatgcaa tcaccgccac
    31021 caggaacgca ccaagataga accacgcaaa ggcgttatgc tgtggcatcg gacgagtttc
    31081 catcaggcgt ttgtaaccca ccatgccgat ggcaaaagtg atattggaga gttgcaccag
    31141 cagcaggcca gtccagaagt ggtcggtgac ctgatcatag cgaataattc cggcaccaat
    31201 caccgccaac aaggcgctaa aagcatagcc ccagcgcaga cggcgcttac tcatgatgtc
    31261 ataaatcagc gtgatgtaga gcggagtcag tacggtgaac agcaacagtt cggagaccgt
    31321 caggtagaga taggcgcgaa aactcagcat atacatcaca ccgagctgca tcgcgcccac
    31381 cagcatatac aggccaaccg ttttcaggct attgccacgg gtacgcagaa acggcagaaa
    31441 cacgagtgcc gccaggccaa cgcgcaccag cactgcaaaa tagctatcga cgtgccccgc
    31501 aaggtactcg ccataaaagc taaaggagaa ggcccacaga atcgtggtga tgataagtag
    31561 cgccacaatg gaaaactctt tgattaacga gtatccattg tagcgaagta tcaggttgac
    31621 aactgaccaa ataaagaacg attacgataa atagagttta cgcagataat gcggcaccgc
    31681 gtcttcggca ttagtaccaa tcacttccag ctccggatgc agatctttca gacgctggtg
    31741 cgcactgccc ataatgcagc ctttacctgc catcgacagc atttcggcgt cgttcatccc
    31801 gtcaccaaag gcaatacaat ctttcagact gtagcccagt ttcttcgcca ccgcttccag
    31861 ggcatggcct tttgaaacgc cgcccgccat cacttccaga caagttaggg tagagaagct
    31921 gacgttgacg cgatcgcccc aacgagcgtt gattgcctgc tccagcggca gcagttgctc
    31981 atgagaatcg caggtgaaga acactttgct gacgccttcc ggctccagta atcctggctc
    32041 atacagcgca tatttgaaca ctgcttcttt aaaaaagcgc atctcttccg ggcggtggcg
    32101 attcataaac cattcgtcgt cgcgataaac gttagtaatg atgtccgggt tgtcgttgac
    32161 cacgccaaac agatcgctgg caatgtcgcg atccaggtta tgagcaaaaa tcagattgcc
    32221 atccagatcg tgaacacgcg cgccattgga ggtaatcatg taagacttaa tctccagatt
    32281 atcgcgaatt tgccctacat caacgtggtg gcgaccggtc gcaaacacaa agttaatgcc
    32341 gcgcgcggtg agcagcttca gagtttcttt ggcgtaaggg gataacgtat ggtcgggaga
    32401 aagtaacgtg ccatctaaat cagacgcaac aacctggtac ataagaaatt taaacctctg
    32461 tagagcggtt tccgctgggt gagttatgcc tgttgaaaaa atcaacaatg gcgtggagcg
    32521 cgactgagcg cattgcgtcc ttttcaaaaa ggatctcatg gtacgcacct ttaattacca
    32581 gcggtcgtcc cccttcgaca ggatggcccg cagcggtgcg gagttcacaa aaacggtcgt
    32641 gcatgcggtt atccaccacg cgttcctctt cagcctgcaa gagaagcgtt ggcgtggcgt
    32701 catcacccgc accggctaac acctgttcgc cagccagaat actttcgcgt acccaatggt
    32761 aggtcggccc accgacgcga atcgttgggt catcagcata aaagcgtaag ttacgtcgat
    32821 atcgctgtct gctgtgggtc agtacgttga tagcaaacgg caacgcgcgc cagcgcccgg
    32881 tgcctattgc atagccatca cggaaacgtg gatgcgcttc ggcccagtta aggatctgcc
    32941 gcgccataaa tgacggcata cgaatcacga tcccaaacat tggcgcagtt agcgcaatgg
    33001 cgtcacatac acctggatgg cgttgcagaa ataacgtgga gatcgcaccg cccatcgaat
    33061 gtgccagtat atagcgctta cgccacggac caggctggac ctcctgttgc cagaatgccg
    33121 ccaggtcatc gacataatca ttaaagcgat taacatgccc aagatgcgga tcggctaaca
    33181 ggcgaccgga acgtccctgc ccgcgatggt cgatgattaa gacatcaaac cccaaatgga
    33241 acaggtcgta ggccagttcc gcatatttta cgtagctctc aatacgcccc gggcagatga
    33301 ctatcacccg gtcatggtgc tgtgcgcgaa aacggacaaa gcgcaccgga atgtcatcta
    33361 caccagtaaa ctctgcttca tcacgctgac gccagaaatc agtcagcggt cccatggtaa
    33421 aagcagcaaa cgcgttttct cttgtttccc agtctttttg ctgctgaaac atcgggtaat
    33481 ctgcctctta aaccacgtaa aatcgttttt ttaagcgtgc ctgacacaac gcttcgacag
    33541 tagcgtattg tggcacaaaa atagacacac cgggagttca tcatgacctt agaatggtgg
    33601 tttgcctacc tgctgacatc gatcatttta agcctgtcgc caggctctgg tgcaatcaac
    33661 accatgacca cctcgctcaa ccacggttat cgcggcgcgg tggcgtctat tgctgggctt
    33721 cagaccggac tggcgattca tattgtgctg gttggcgtgg ggttggggac gctattttcc
    33781 cgctcagtga ttgcgtttga agtgttgaag tgggcaggcg cagcttactt gatttggctt
    33841 gggatccagc agtggcgcgc cgctggcgca attgatctta aaccgctggc ctctactcaa
    33901 tcgcgtcgac atttgttcca gcgcgcagtt tttgtcaatc tcaccaatcc caaaagtatt
    33961 gtgtttctgg cggcgctatt tccgcaattc atcatgccgc agcagccaca actgatgcag
    34021 tatatcgtgc tcggcgtcac cactattgtg gtcgatatta ttgtgatgat cggttacgcc
    34081 acacttgctc aacggattgc gttatggata aaagggccga aacagatgaa ggcattgaat
    34141 aagatttttg gctcgctgtt tatgctggta ggagcgttgt tggcatcggc gaggcatgcg
    34201 tgaaaaataa tgtcggatgc ggcataaacg ccttatccga cttactctga tgaagcgttt
    34261 ggcatcaacg agaaataatc aaatgaatgc caaatccggc aaataacgcc ccggcaaaac
    34321 catcaatcca cttcgccaga cgttgataac cacggcgcat ttgcggtaga gcaaacaggc
    34381 tggcaaccac cgtaaaccat gccagtgttt cgataataat caacgcgaaa atgccccagc
    34441 gctcagtggt gccgacgtta tcgccgacaa acagcgagaa taccgaacca aagtagataa
    34501 ttgctttcgg attggcgaga ttggtcagta agcctttcag gaaactgcgc ccgctttttg
    34561 ccagttctac ctgtggcgca ggcgcagaaa cgacctcttt tttcagtgcg ctacgcagca
    34621 tctgataacc catccagcag agatacaggc caccgcccac cataatcagc gtatgcagcc
    34681 aggccatttt ttcgataatc aaatgcaggc caagcaacgc gatccctgcc cagaccatta
    34741 cgccgcaggt aatgcccagc acgcccatca tcgcctcttt acgggaacga ctgacagcgg
    34801 tctgagaaac aaagaagaaa tcgggaccgg ggctcataag cgccacaatg tgcaccatgg
    34861 caacggtgag gaataacatc aacattcaat tgactcgcgg agaaatcgtt tacggagtct
    34921 tcatcctggc acttttttat gctgctgact actcttcgtc atcgccatcg acatgcgcac
    34981 gaattagcgc cataaacggt ttgccaaagc gttccagctt gcgcatccca acgccgttaa
    35041 cgctgagcat ttcgctggcg gagatcggca tctgttccgc catctcaatc aaggtcgcat
    35101 cgttaaacac cacatacggc gggacattac tttcatcggc aatcgattta cgcagtttgc
    35161 gtaatttggc gaacagtttg cgatcgtaat tgccaccgaa agatttctgc attgctttcg
    35221 gtttgagcgc cacaatacgc ggcacggcaa gttgtaaaga ggattcgccg cgcaacaccg
    35281 ggcgtgcggc ttctgtcagt tgtaatgccg aatgctgggc gatgttttgc gtcaccagac
    35341 cgagatgaat caactggcgg attacgctca cccagtgctc atgactttta tcgcggccca
    35401 tgccgtagac tttcagtttg tcatgaccat agtcgcggat tcgctggtta ttggcgccgc
    35461 gaatcacttc cactacatag cccatcccaa aacgctgatt cacacgacca atggtggaaa
    35521 gcgcaatctg cgcatcggtt gaaccatcgt attgtttcgg cggatcgagg cagatatcgc
    35581 agttcccgca cggctcctgc cgcccttcgc caaagtagtt cagcagcacc agacgacggc
    35641 aagtttgcgc ttcggcaaaa gcgcccatcg cattgagttt atggcgctcg atatcctgca
    35701 actgcccctg cggcttctct tccagacaac ggcgcagcca cgccatatca gccggatcgt
    35761 aaaacagcat cgcttccgcg ggcaggccat cacgcccggc gcgaccggtt tcctgataat
    35821 aggattcgat attgcgtgga atatcaaagt gaaccacgaa gcggacgtta ggcttgttga
    35881 tgcccatgcc gaacgctacc gtcgccacca caatttgcag gtcatcacgc tggaattttt
    35941 cctgcacgtc cgcacggaca tcgttttcaa gcccggcgtg ataagccgct gcgctgatac
    36001 ccttgctttg cagacgcgcg gcggtgtctt ctactttcgc acgactatta cagtaaataa
    36061 tccccgactt accgcgttgt tcctgtacat aacgcatcaa ctgatcgagc ggtttgaatt
    36121 tctccatcag catgtagcga atattcggac ggtcaaaact gctaatttga atcagcggat
    36181 cgttcagccc cagcaggcgc acgatatcct gccgcgtggt gtcgtcggcg gtggcggtta
    36241 gcgccataaa cggcagcgtc gggaaccgct ggcgcaactg accgagcgcg gcatattccg
    36301 ggcggaaatc gtggccccat tgggagatac agtgcgcttc atcaacggct aataacaccg
    36361 gattccagtg cgccagatgc tcaagaaagt tatccagcat caggcgttcc ggggcgatat
    36421 aaagcagacg aatttgcccg gtgcgacagc ctgtcatcac ttcaagctgc tgctcgcggg
    36481 tttgcgtcga gttaaggcac gccgccgcca caccgttggc ctgtagctga tccacctgat
    36541 ctttcatcaa cgaaatcagt ggtgaaacaa ccacggtaag accgtttagc agtaaggcag
    36601 gaatttgata gcaaagggat tttccgccac cagtgggcat gacgaccagg caatcgcgac
    36661 cggaaagtac ggtgtcgata atctcttcct ggccggggcg aaactgttgg tagccaaagg
    36721 tttcttgtaa aacctgttta gctccggact ccagattcaa cacttccgcc tgcgccacat
    36781 taaccccgat tgcctgaaat aaaaacaggc gctattttca gcgcctgcgc gagaaactgc
    36841 aatgctttta gtctctgaga gggtaatcct ggatactgga ttccagtttt gcctttcaat
    36901 caactcacat tagaacaagt cgttcagcat aacccccaca cccacacggg tctggttaaa
    36961 gttatagtcg atgagcgatt cgccataacc gctgtaaacc tgggtataaa ggcgcacatg
    37021 cctggtgatc gggtaactta agcctaactc cgcaccaccg taaccggtgt tccagttgta
    37081 ctgtcctttc gcactcagca ccgcatcacc caggtgatag ccgattttaa gctggtagta
    37141 acccatatat ttggtgatat ctgggttatc gtcagtattc cccaccacat accacggctt
    37201 cacctctacc agccagttac cgttttctgc catcagacgg gtataaaggc ggttccagct
    37261 gcgggaagtc gggtctgaac gcccgttaga gtcgtggtta taacccatct ccacatcgcg
    37321 cagcgtccag ccagcaaagc ggtaatcggt ggcaaaacca aggaacaatt gcggttcgta
    37381 gttggtttca cgaaacggtg aagactcttc gctattggac agttgccacc aggatttttg
    37441 cgtgtaagaa gcccccaaca ccgagtttgg ccccaatatt ccgcgccaca gcggaaatgc
    37501 caggctcaac tggaatttca cttcatcctt acgcgcgttt tccgcccagt cataactggc
    37561 gatcgcttct ttgttcagat cgctggtttg ggtgtagatg aggtagttgg tgtcataagg
    37621 atagagcgtg aaaggattgt catgctcctg cagcatattc gcaataatac tgccacgcac
    37681 cgcaggcgcg tcatgcacct cttttaccgt tgcctcttgc gcatacactg ccattggcaa
    37741 cataaacacc ggcaacaacc agccctgcag agtccgcatc ggtcgtgttc tccgtaacga
    37801 attattgatt agatgaatat ttttctgcgg gctattttac atacttaact cctttgccgt
    37861 tagttatccc ttcctaaagt agaaaaccac aattaagaag cataaaatca acatttcatt
    37921 aaccaatgag gtgtaaggat gatatgtctg ccgtactgac cgctgaacaa gccctgaaat
    37981 tagtgggtga gatgtttgtt tatcacatgc catttaaccg cgcattgggg atggaactgg
    38041 agcgttacga aaaagagttc gcacagctgg cctttaaaaa tcagccaatg atggtgggta
    38101 actgggcgca aagcattttg cacggcgggg tcattgcgtc ggcgctggat gtcgccgcag
    38161 gtctggtgtg cgtgggaagt accttaaccc gccacgaaac catcagtgaa gatgaactac
    38221 gccagcggct atcgcggatg gggaccattg atcttcgcgt cgattatctg cgcccaggca
    38281 ggggcgagcg ttttactgct accagtagtc tgttgcgtgc aggcaataaa gtcgccgttg
    38341 cccgcgttga attacacaat gaagaacagc tttatatagc cagtgccacc gccacctata
    38401 tggtaggttg ataacgtaaa agcaggtaaa ctgcttttac caataaaaaa tgagttttgc
    38461 caatggacac caaacaaacg cggcagggcg tattactcgc tcttgccgct tattttattt
    38521 ggggtattgc gccagcgtac ttcaagctga tttactacgt gcccgccgat gagatcctga
    38581 cgcatcgcgt gatctggtcg tttttcttta tggtggtgct gatgagcatt tgccgccagt
    38641 ggtcctattt aaaaacgctg attcagacac caaagaaaat cttcatgctg gcagtctctg
    38701 ccgtgctgat tggtggcaac tggctactgt ttatctgggc ggtgaacaat caccatatgc
    38761 tggaagcgag ccttggttac tttattaacc cgctggtgaa cattgtgctg gggatgattt
    38821 tcctcggcga gcgattccgc cggatgcaat ggctggcggt gattctggcg atatgtggtg
    38881 tattggtcca gctctggact tttggttcgc tgccaatcat cgcgctgggg ctggcgttta
    38941 gttttgcctt ctacggcctg gtacgcaaga aaattgccgt tgaagcgcaa accggcatgt
    39001 taatcgaaac catgtggctg ctgcccgtgg cggcgattta cctgtttgct attgccgaca
    39061 gctcaaccag ccatatgggg caaaacccga tgtcgctgaa tttactgctg atcgccgccg
    39121 gtattgtcac caccgtgcca ctgttatgtt ttaccgcagc agctacacgc ttacgactct
    39181 caacattagg ctttttccag tacattgggc cgacgctaat gttcctgttg gcagtgacgt
    39241 tttatggtga aaaaccgggt gccgataaga tggtgacttt cacctttatt tgggtggcgc
    39301 tggcaatttt tgtgatggat gcgatttata cgcag
//
LOCUS       DAZGTJ010000038        38915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000038 DAZGTJ010000000
VERSION     DAZGTJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.038"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            116..2758
                     /locus_tag="ACHK8E_002439"
                     /pseudo
     CDS             116..2758
                     /locus_tag="ACHK8E_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
     gene            complement(2816..3151)
                     /gene="ymgD"
                     /locus_tag="ACHK8E_002440"
     CDS             complement(2816..3151)
                     /gene="ymgD"
                     /locus_tag="ACHK8E_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP0344696.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3155..3499)
                     /gene="ymgG"
                     /locus_tag="ACHK8E_002441"
     CDS             complement(3155..3499)
                     /gene="ymgG"
                     /locus_tag="ACHK8E_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP0344697.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3501..3674)
                     /locus_tag="ACHK8E_002442"
     CDS             complement(3501..3674)
                     /locus_tag="ACHK8E_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344698.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3775..3960
                     /locus_tag="ACHK8E_002443"
     CDS             3775..3960
                     /locus_tag="ACHK8E_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344699.1"
                     /translation="MSRKYSSAMITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3921..4040
                     /locus_tag="ACHK8E_002444"
     CDS             3921..4040
                     /locus_tag="ACHK8E_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0344700.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            4075..4419
                     /locus_tag="ACHK8E_002445"
                     /pseudo
     CDS             4075..4419
                     /locus_tag="ACHK8E_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4784..5050)
                     /gene="minE"
                     /locus_tag="ACHK8E_002446"
     CDS             complement(4784..5050)
                     /gene="minE"
                     /locus_tag="ACHK8E_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP0344701.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5054..5866)
                     /gene="minD"
                     /locus_tag="ACHK8E_002447"
     CDS             complement(5054..5866)
                     /gene="minD"
                     /locus_tag="ACHK8E_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP0344702.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5890..6585)
                     /gene="minC"
                     /locus_tag="ACHK8E_002448"
     CDS             complement(5890..6585)
                     /gene="minC"
                     /locus_tag="ACHK8E_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP0344703.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSAIGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7104..7472
                     /gene="ycgJ"
                     /locus_tag="ACHK8E_002449"
     CDS             7104..7472
                     /gene="ycgJ"
                     /locus_tag="ACHK8E_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP0344704.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7575..7976)
                     /gene="pliG"
                     /locus_tag="ACHK8E_002450"
     CDS             complement(7575..7976)
                     /gene="pliG"
                     /locus_tag="ACHK8E_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP0344705.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8218..8511
                     /locus_tag="ACHK8E_002451"
     CDS             8218..8511
                     /locus_tag="ACHK8E_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP0344706.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8583..9242
                     /gene="ycgM"
                     /locus_tag="ACHK8E_002452"
     CDS             8583..9242
                     /gene="ycgM"
                     /locus_tag="ACHK8E_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0344707.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9334..9780
                     /gene="ycgN"
                     /locus_tag="ACHK8E_002453"
     CDS             9334..9780
                     /gene="ycgN"
                     /locus_tag="ACHK8E_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP0344708.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(9987..10313)
                     /gene="hlyE"
                     /locus_tag="ACHK8E_002454"
                     /pseudo
     CDS             complement(9987..10313)
                     /gene="hlyE"
                     /locus_tag="ACHK8E_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            10574..10993
                     /gene="umuD"
                     /locus_tag="ACHK8E_002455"
     CDS             10574..10993
                     /gene="umuD"
                     /locus_tag="ACHK8E_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0344709.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            10993..12261
                     /gene="umuC"
                     /locus_tag="ACHK8E_002456"
     CDS             10993..12261
                     /gene="umuC"
                     /locus_tag="ACHK8E_002456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP0344710.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(12306..12836)
                     /gene="dsbB"
                     /locus_tag="ACHK8E_002457"
     CDS             complement(12306..12836)
                     /gene="dsbB"
                     /locus_tag="ACHK8E_002457"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP0344711.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(12982..14523)
                     /gene="nhaB"
                     /locus_tag="ACHK8E_002458"
     CDS             complement(12982..14523)
                     /gene="nhaB"
                     /locus_tag="ACHK8E_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP0344712.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            14745..15464
                     /gene="fadR"
                     /locus_tag="ACHK8E_002459"
     CDS             14745..15464
                     /gene="fadR"
                     /locus_tag="ACHK8E_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP0344713.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(15516..17048)
                     /gene="ycgB"
                     /locus_tag="ACHK8E_002460"
     CDS             complement(15516..17048)
                     /gene="ycgB"
                     /locus_tag="ACHK8E_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP0344714.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            17378..18676
                     /gene="dadA"
                     /locus_tag="ACHK8E_002461"
     CDS             17378..18676
                     /gene="dadA"
                     /locus_tag="ACHK8E_002461"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP0344715.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            18686..19756
                     /gene="dadX"
                     /locus_tag="ACHK8E_002462"
     CDS             18686..19756
                     /gene="dadX"
                     /locus_tag="ACHK8E_002462"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP0344716.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(19813..21549)
                     /gene="cvrA"
                     /locus_tag="ACHK8E_002463"
     CDS             complement(19813..21549)
                     /gene="cvrA"
                     /locus_tag="ACHK8E_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP0344717.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(21644..22558)
                     /gene="ldcA"
                     /locus_tag="ACHK8E_002464"
     CDS             complement(21644..22558)
                     /gene="ldcA"
                     /locus_tag="ACHK8E_002464"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP0344718.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            22658..23269
                     /gene="emtA"
                     /locus_tag="ACHK8E_002465"
     CDS             22658..23269
                     /gene="emtA"
                     /locus_tag="ACHK8E_002465"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP0344719.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(23271..24005)
                     /gene="ycgR"
                     /locus_tag="ACHK8E_002466"
     CDS             complement(23271..24005)
                     /gene="ycgR"
                     /locus_tag="ACHK8E_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP0344720.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            24206..24460
                     /gene="ymgE"
                     /locus_tag="ACHK8E_002467"
     CDS             24206..24460
                     /gene="ymgE"
                     /locus_tag="ACHK8E_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0344721.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(24510..26480)
                     /locus_tag="ACHK8E_002468"
     CDS             complement(24510..26480)
                     /locus_tag="ACHK8E_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0344722.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(26506..27360)
                     /gene="modD"
                     /locus_tag="ACHK8E_002469"
     CDS             complement(26506..27360)
                     /gene="modD"
                     /locus_tag="ACHK8E_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP0344723.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(27357..28169)
                     /locus_tag="ACHK8E_002470"
     CDS             complement(27357..28169)
                     /locus_tag="ACHK8E_002470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0344724.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(28179..28937)
                     /locus_tag="ACHK8E_002471"
     CDS             complement(28179..28937)
                     /locus_tag="ACHK8E_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0344725.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(28934..29914)
                     /locus_tag="ACHK8E_002472"
     CDS             complement(28934..29914)
                     /locus_tag="ACHK8E_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0344726.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(29914..30936)
                     /locus_tag="ACHK8E_002473"
     CDS             complement(29914..30936)
                     /locus_tag="ACHK8E_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP0344727.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(31273..32970)
                     /gene="treA"
                     /locus_tag="ACHK8E_002474"
     CDS             complement(31273..32970)
                     /gene="treA"
                     /locus_tag="ACHK8E_002474"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0344728.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(33291..34709)
                     /gene="dhaM"
                     /locus_tag="ACHK8E_002475"
     CDS             complement(33291..34709)
                     /gene="dhaM"
                     /locus_tag="ACHK8E_002475"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP0344729.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(34720..35352)
                     /gene="dhaL"
                     /locus_tag="ACHK8E_002476"
     CDS             complement(34720..35352)
                     /gene="dhaL"
                     /locus_tag="ACHK8E_002476"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP0344730.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(35363..36433)
                     /gene="dhaK"
                     /locus_tag="ACHK8E_002477"
     CDS             complement(35363..36433)
                     /gene="dhaK"
                     /locus_tag="ACHK8E_002477"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP0344731.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            36661..38580
                     /gene="dhaR"
                     /locus_tag="ACHK8E_002478"
     CDS             36661..38580
                     /gene="dhaR"
                     /locus_tag="ACHK8E_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP0344732.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(38617..>38915)
                     /locus_tag="ACHK8E_002479"
     CDS             complement(38617..>38915)
                     /locus_tag="ACHK8E_002479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344733.1"
                     /translation="CYAASFNSELSKIIIRLLAILTMCLLCDNNHSALTVQEFVMKKT
                     FTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
BASE COUNT         9795 a         9431 c         9959 g         9730 t
ORIGIN      
        1 tctatttaaa ttttttgtgc ttttgttttt gtctttaccc tccgttttgg taatctgaac
       61 tcataggtta tttgaccagg aatgtttgtt aatccaatga atggatggtt aatctatgaa
      121 actgaaaaaa ctccccgggt ttagtttagg acttattgct ttggcggtgg gtaatgcata
      181 tgctgaacag tttattcagc acgatgaacc taaacatgtt gttgaaataa aagatggaga
      241 agtaaaagat attgataatg cgctagttat aacaacgggt aatggaagtt acggtgtatc
      301 tgttgcagga aagggtagca atttaaccat taacaatggc gtcattgtaa caacgggcgg
      361 tttatatcct tctgacaata gttctctaag cacagcatat acagctagcg cagtcgtaag
      421 tgaatatggc ggtgctgtca ctttgcgggg taataatacg attgtcacga cgggagatta
      481 ttctgttggt ttgttaagtc aagttgatgg aaacctgaat actgatacca taataagagt
      541 caaccctgac ggttcggcta ccccctcttt ttctgacgga ggtaatactt ttatcgttac
      601 tgcgggtaat cacgcagtcg gcgttcttgc gtgtgcatct cccggaagcg cgcgtgcgtg
      661 tgtatcttct cttgatgaag aaagtactgc cgatacagga agtaatgaaa ataacgccaa
      721 agcaaaactg gatatggcaa aaggggagat aacaacccac ggaacagaaa gttatgccgc
      781 ttacgctaat ggcactgtcg tcaaggaggg tagcaagctt gattatacca atgccagcgt
      841 tacattaacc gatgttgata tcaccacgca tggggacaat gctcatgcca tcgctgctcg
      901 ccaggggaca gtttcattta accagggaga aatttataca acgggtcctg acgccgcgat
      961 tgctaaaatt tataatggcg gcaaggtgac gctgaaaaat acatctgcag tcgcgcatca
     1021 gggggctgga attgtgctgg agtcttccat aaatggtcag gaagcaacgg tagatatttt
     1081 atctggtagc tcactacggt cagcaaatga aatcctctac cataaaaatg agacgagtaa
     1141 cgtgaccatt acggatagtg aggtatcatc ggctgcagat gtttttatta ataatattaa
     1201 aggtcatttg accgtcgatg caactaattc aaaaataacg ggttcagcca atctttcaac
     1261 agatgataac actcatacct atctgtcgct ttcagataat agtacctggg atattaaagc
     1321 tgactcaacg gtgagcaaac tcacggttga taatagtacg gtctatattt cccgggcaga
     1381 tggaaaggat ttcgaaccaa caagattgac aataactgaa aattacgttg gtaataatgg
     1441 cgtattgcat ctcagaactg aattgggtga tgataattca gctacggata aagtcgttat
     1501 taatggaaat acctctggaa caacccgagt taaagttacc aatgctggcg gcagcggggc
     1561 ttacacgtta aatgggatag agattatcag cgttgagggg gaatcaaatg gagaatttat
     1621 taaggattcg aggattttcg ccggtgccta cgaatattca ttaacccgag gtaataccga
     1681 agcgaccaat aaaaactggt atctgactaa cttccaggca acgagcggcg gtgaaacaaa
     1741 ctccggagga agttcagcgc ctactgttgc gcctaccccc gtcctgcgcc ctgaagctgg
     1801 aagttacgtc gccaacctgg cagccgctaa cactcttttt gttatgcgtc tgaacgatcg
     1861 tgcaggtgaa acgcgttaca tcgatcctgt aacttaacag gagcgttcaa gccggctttg
     1921 gctacgtcaa attggcgggc ataatgcctg gcgtgacagc aacggacagt tgagaacgac
     1981 ctcgcatcgc tacgtctcgc agttaggggc ggatctgtta accggtggtt ttaccgacag
     2041 tgacagttgg cgtttgggag tgttggctgg ttatgcccgc gactacaact caactcattc
     2101 cagcgtgtcg gattatcgtt cgaaagggag tgtcagaggc tatagcgcag ggctgtatgc
     2161 cacctggttt gccgatgaca tcagtaaaaa aggcgcatac attgacacct gggcgtaata
     2221 tagctggttt aaaaactcgg tgaaagggga tgaattagcc tatgaatcct atagcgcgaa
     2281 aggtgcaacc gtctcgctgg aagcgggtta cggttttgcc ctgaataaat cctttggtct
     2341 ggaagcggcg aaatatacgt ggctcttcca gccacaggct atctggatgg gcgtcgatca
     2401 taatgcgcac acggaagcca atggctcacg tattgagaat gacgcaaata acaacatcca
     2461 gacccgactc ggcttccgca cctttattcg tactcaggag aaaaacagcg gtccgcacgg
     2521 tgacgacttt gaaccttttg ttgaaatgaa ctggatccat aacagtaaag attttgctgt
     2581 ctcaatgaac ggtgtgaaag ttgaacaaga cggggcgcgt aatctggggg aaattaaact
     2641 tggcgtaaat ggcaatctga atccggcggc cagcgtctgg ggtaatgtgg gcgtgcagct
     2701 gggggataat ggctacaatg acaccgcaat gatggtgggc ctgaaatata agttctgatc
     2761 ccgccattag ctaaaaaacc gcgtcgaatt catcgacgcg gtaacatatg aaatattatt
     2821 ttttgccgat agcacgcatg gtgtcatcaa ttgccgtgat caacagcatt tgcgggtctt
     2881 tagcgcaaac ctgattcagt ttttctacca ctttggcgct tagttccgga gattgctcaa
     2941 tttttaaatc acggctggca acgctggcat tacccattac cgcaacaatt tctgcaacct
     3001 gtgcgctgtc agtttttgcc atttcgttgg cttctgcgca agtaatatag gtttctgacg
     3061 gcaaaccgtt tttaatattg tagtcctgcg cccaggtcat tggtgcgaaa acaaacagac
     3121 ccgccagtaa agcaaatttt ttcatcatca ttccttattt cattttaccc agaattgcac
     3181 cacccgtacc gccaatcacg gcacctttaa tcgccccttc gaggccattg ccggtcagaa
     3241 cgccagtgac agcaccaacg gcggcaccca cttttgcacc tttacgcgca tttttaccgt
     3301 cgcggccttt ttctgttact gcaccaacac cagcgccaac agctgcacct ttcagtacgc
     3361 cattaacacc attgccagta agtaaaccaa cgcctgcgcc tagcaatgca cctttcgtgg
     3421 tgcggttcat atccgccatc gctggcgtgg agcagaacaa tgctgagata agcccgaagg
     3481 caagtatttt tttcttcaac ttagatgtcc ggtattaagt aagttgcaca cacaataatt
     3541 tcgtcttcaa ttaagatctg cttaactaaa gaacgctcgc tattattcag ataattcaaa
     3601 atgagcgtga ctgtgatgat aggaattatg ttttttacgg gaataagaat aatctcaaat
     3661 gataaataac tcattgattg acaatatttt tattcaagaa gtgtcattga gtgttaatac
     3721 aatgttgtaa aggtaagata atctgattta tgaatattat tgtgtgattt ttatgtgagc
     3781 agaaaatatt catcagcaat gattacatta gtcattttat tttgtcgacg gcctcattgt
     3841 cgaaaaataa gcgtacaaca gtattatcag aaaagagtga ttttttatcc aactacactt
     3901 cagcgcactg cgtttaaaaa atgcctcttt cttatgcggg atatcattat ttcatcatga
     3961 tgtctttggt gagcggtgaa cacaatacac ttgcgctgcc tattcaggag caatcccctg
     4021 gtctggtgcc tgcgggatga tgttgcagca gagctaaggt cacttagacg ctactatcat
     4081 ttatccaatg gcatggggtc gaaatcagtc gatacccgca gtatatatcg tgaacttggt
     4141 gcaacgctga gttacaacat gcgtctgggg aacggcatgg aagttgagcc gtggctgaag
     4201 gcggctgtgc gcaaagaatt tgtcgatgat aaccgggtga aagtgaataa tgacggtaat
     4261 tttgtcaatg atttgtcggg cagacgtgga atatatcagg caggtattaa agcctcattc
     4321 agcagttcgt taagcgggca tctcggggtg gggtatagcc atggtgccgg tgtggaatcc
     4381 ccatggaacg cggtggctgg tgcgaactgg tcgttctgac catcaacgat taaactgcgc
     4441 ttcgcgcagt tttcgtttac cgggtgctaa aagggaaaat tctgcggcaa aaaaagcccg
     4501 ccagttacgg cgggaaacct catcctatgg gagaacaatg aataatgaaa ttgcggggtt
     4561 atcatctccc agtataccca tactaacaat aaggttattt actcagccag gtataaacaa
     4621 tttgttctgt gtggggggaa cggcattaag atgtaaaggg ggggaagcaa tgagtagcat
     4681 cagcaagaat actcgccgct cttatcaaac gtggatgagg gggatgaaaa actcaaggca
     4741 gagatgactc tgccttgaag ataaatgcgc ttttacagcg ggcttatttc agctcttctg
     4801 cttccggtaa ggtcacgttc agctcaagaa tagaaatatc gccatctttt tgctcaagct
     4861 gtacggttac catctcagga tcaatttgta cgtatttaca aatgacctca agaatatctt
     4921 tacgcaactg cggcagataa tgcggttctg catcactgcg acggcgttca gcaacaataa
     4981 tctgcagccg ttcttttgca atgttggccg tgtttttctt ccgcgataga aagaaatcga
     5041 gtaatgccat aacttatcct ccgaacaagc gtttgaggaa gcctttcttc tcttcttcaa
     5101 tgaagcggaa aggacgttct tctcccaaca gacgttctac ggtatctgcg taggctttac
     5161 ccgcatcggc gttaatgtcg agaatgaccg gttcaccctg gttagaggcg cgcaatactg
     5221 attgatcctc agggatcacg ccgacgagtt tgatgcgcag gatctccagc acatcttcca
     5281 tgctcagcat gtcacctctg cttacgcggc ctgggttata gcgcgttaac agcaggtgct
     5341 ctttaatagg ctcttcgcca ttttctgcgc ggcgtgattt cgacgccaga atgcctaaaa
     5401 tacggtcaga gtcgcgtact gaggagactt ccgggttggt ggtaataatg gcttcgtctg
     5461 caaaatagag tgccattagc gcaccggtct caatccctgc cggggagtcg caaacaataa
     5521 attcaaagtc catcgctttc agatcatcaa gaactttggc gaccccttca cgggtgaggg
     5581 catctttatc gcgagtttgc gatgccggaa gaatatagag attttcagta cgcttatctt
     5641 taattaacgc ctgatttagc gttgcatcgc cctgaatgac gttgacgaaa tcgtaaacga
     5701 cccgacgttc acaacccata atcaggtcga gattacgcag gccgatatca aaatctatca
     5761 cgacagtttt ctttcccttc tgggccaaac cagtggcgat ggccgcgctg gaggttgtct
     5821 taccaacacc ccctttgccc gaagtaacaa caataatgcg tgccatagaa attccttgtt
     5881 aaaaagggat caatttaacg gttgaacggt caaagcgttt tcgactaact gcagtcgcgc
     5941 cgctttgcca taaaattctg ctgggatttg atcactcagc cagtattcac ctgcgatgga
     6001 caccagttcc gccatcaggt tcgtacaaaa tatttgcgtt tcccggtcac cacttgcccc
     6061 tgccagcgca cgaccgcgca tcatgccata gacgtgaata ttaccatcgg caatcaattc
     6121 ggccccagcg ctaacgtggc ttgtaacaat cagatcacat tgtggagcat aaatacgctg
     6181 accggaacgc accggggtat ctattaaacg cgtttttgtg accggcgttg tattttgcgc
     6241 tggagcctgc ggtgcgggag ctggacgcgg cgctttttcc tttccttccg tcaggatagg
     6301 cagccccatc ttttcaattt cggctttaag ttgcgcatct ttgcagccac ttacgccaat
     6361 aacccgcaaa ccgattgccg aaaccgcctt atgcatcgct gaccagttta ccgggtcttc
     6421 cagtgcactg acgttgagta caacgggggc atgttttaaa aatgcggggg cctgagcgat
     6481 tttgtcttcc agcgcctgat ggataacctt aggttctgcc tcatgcagat gaaccacaga
     6541 taaagtgaag ctactgcctt taagctcgat tggcgtgttt gacatcctgg ccttactcaa
     6601 ttagctatta atcatcgcca gcgcgcgatg atgttccgaa gactataagg catgttatag
     6661 tctggattat attgaggcaa gtcaccctcc catttattca gagtaaaagt ctattctgtg
     6721 ataaatggcg ctgattcata gcttaaaaaa tacccttgtc aatcaaccca ttgccgtcgt
     6781 acttttgatt gttcttattt acgcttcctt tttccgcacc ctaatcgtga cagcatcttc
     6841 agacctgttt aaatatccag tattgaggcg agtgtatttt ttcaccacgc caggctttct
     6901 taactttcca tttttgcagc atcattaaag aacagatacc cgttatctta atatccgcca
     6961 cagaattgct ctatcataga aaccgcagaa gacgtctgat acttctgtca ggtcatcgta
     7021 actcgtccag gtcagcggtt ttgattacgg attgatgatg aaatgagcat aagtgttgta
     7081 cggttggatt atcaggatgt gctatgaata tgatgcgtat tttttatatc ggattgtcag
     7141 gtgtggggat gatgttctca tcaatggcgt ctggacatga cgctggcgga cttcaatctc
     7201 cggcgtgcgg cgttgtttgc gacccgtata tttgtgttaa ctcagatggc atttccccag
     7261 agttaacaag gaaatatctc ggcgaaaaag ccgctgaaaa cttacaatca ttacaaggct
     7321 acgatcccag cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat
     7381 gtcgtgatga tcgttatttt ggtgtgaatg gtaagcggag tggaaaaatc aatcaaacca
     7441 ccacaaagat gttatttatg tgtcgtgagt gatgtcttgt ttacattatg ttctattcgt
     7501 cattaacctc ttgagcgaca gagaggagtg gggcgttaac gtttatgacg gggagagtcc
     7561 ccgtcggttg atatttactt aatttgaata tcgacgttat attttttagt tttgttttta
     7621 cgggcgtcgt ttcgggtttg cagcactcgc aactcatatt tgccagaagc agggagtgag
     7681 tattgtccgt gactatcgag ttctggggaa tatctggata aatcaacaga atcatcgatt
     7741 cctggcccga agaggtaagt atcagcgcct tcattagaga tactcacatg cactttctga
     7801 ccttttttgg catagaaggt atatgtatcg taatcgtatc cctttatttc gccggagtac
     7861 tgagcgctgc tatggccttt tctgaactca acattgacat tcttacccgc agcaaagctc
     7921 gtggaggtta gcagtgcaag caacaataca gccttactga tgcccttaat tttcattggt
     7981 agtctcctgt tcagaatgag tacgatgaac tgttataata taacaattca tcacaggaga
     8041 attccagtgg ctttcctgcg ttcggttaaa tcccggcgcg gcatttttgc cggggggatt
     8101 acttataaag ggggcatgga ctgaacaaac cgtaggaatc tacagctggc tggcttgtgg
     8161 cgcgggtttc attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg
     8221 ttttgtgtga tttatcgaag cagcaagcgt gatcagacct atttatatgt cgaaaaaaaa
     8281 gacgattttt cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg
     8341 atgattcttc cgctggatgg gcggaagaaa ctggtcaatg ccgatattga aaaggttaaa
     8401 caggcattaa ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg
     8461 aagcaacatc tttccgtcat ggggcagaaa acagacgaca cgaacaaata accgatatcc
     8521 ggcggtggca ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg
     8581 ttatgtatca acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag
     8641 tctgtgttgg tagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag
     8701 agccagtgct gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga
     8761 tcccatccga tttcggttca gttcatcatg aagtcgaact ggcggtattg attggcgcga
     8821 cgctgcgtca ggctacggaa gagcatgtcc gtaaagccat tgccggttac ggcgtggcgc
     8881 tcgatctgac attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa
     8941 aggccaaagc gtttgataac tcttgtccgc tttccgggtt tattcccgcg gcggaattca
     9001 ccggcgatcc gcaaaataca acgctgggcc tgagcgtaaa cggcgaacaa cgccagcaag
     9061 gtacgactgc ggacatgatc cataaaatcg ttccgctgat cgcttatatg agcaagttct
     9121 ttaccctcaa ggccggtgac gttgtgctga caggcacgcc tgatggcgtc ggcccgttgc
     9181 aaagtggtga tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt
     9241 aatacttttt gccgcctgaa agcggcggca aaacttgcat cgctgtgcca gactggttat
     9301 aaggtgcgtt ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt
     9361 aaaaccctgg acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag
     9421 tgttgcctgc ataagctgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc
     9481 tgtcgccagc tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt
     9541 gaacctgact gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg
     9601 acctgcgctt atcgcttgct ggcggaaggt aaagatttac ctgcgtggca tccgctgctt
     9661 actggttcga aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa
     9721 gaatcagaag tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga
     9781 taaatttaat agatgttgat gggggctttc ttaatatacg tctggatact cgccatttcg
     9841 tagccctgtt tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac
     9901 aggtaactct caatctttaa aaaacggctg tgtttttaaa tgtgataaac cttatacctt
     9961 gagtacatgg agagaataat cgcttatcag acttcaggta cctcaaagag tgtcttttta
    10021 ccgtgtcttt tctgatactc attacaggtg ttaatcattt ttttggccgc ttcttttagc
    10081 aaagaaagca ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt
    10141 atctccccga tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta
    10201 ttcgcttgtt taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg
    10261 ttcttcaatt ctggaatcag tttcccttca actacgcccg ctgcaacaga atacagtttc
    10321 ttgatgccgt catccagtac cttaatgaga atgcctttct gggcgaatgc ttctattcta
    10381 ttttttagcc gggcgatatt tttcatttct gctggatgag cgtcgtcgcc agaaggccac
    10441 gtgagcacag gataagagaa cgaaaaatca gcagtctatg cagcgacaaa tattgatagc
    10501 ctgaatcagt attgatctgc tggcaagaac agactactgt atataaaaac agtataactt
    10561 caggcagatt attatgttgt ttatcaagcc tgcggatctc cgcgaaattg tgacttttcc
    10621 gctatttagc gatcttgttc agtgtggctt tccttcaccg gcagcagatt acgttgaaca
    10681 gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc gcgacttact tcgtcaaagc
    10741 aagtggtgat tctatgattg atggtggaat tagtgacggt gatttactga ttgtcgatag
    10801 cgctattacc gccagccatg gtgatattgt catcgctgct gttgacggcg agtttacggt
    10861 gaaaaaattg caactacgcc cgacagtgca gcttattccc atgaacagtg cgtactcgcc
    10921 cattaccatc agtagcgaag atacgctgga tgtctttggt gtggtgatcc acgtcgttaa
    10981 ggcgatgcgc tgatgtttgc cctctgtgat gtaaacgcgt tttatgccag ctgtgagacg
    11041 gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc tatcgaataa tgacggttgc
    11101 gttatcgccc gaaacgctga ggcaaaggcg ctgggcgtta aaatgggcga tccctggttc
    11161 aaacaaaaag atctgtttcg tcgctgtggc gtggtttgct ttagcagcaa ttatgagctt
    11221 tacgcagaca tgagtaatcg ggtgatgtcg acgctggaag aactatcgcc ccgcgtcgag
    11281 atttacagta ttgatgaggc attctgcgat ctgaccggtg tgcataattg tcgcgatctg
    11341 actgattttg gcagagaaat tcgcgcaacg gtgctacaac gtacccatct tactgttggt
    11401 gtggggatcg cccagaccaa aacgctggct aagcttgcca atcatgcggc aaaaaaatgg
    11461 cagcggcaga cgggcggggt ggtggattta tcaaatctgg aacgccagcg taaattaatg
    11521 tctgctcttc ccgtggatga agtctggggg attggacggc ggatcagcaa aaaactggac
    11581 gcgatgggga tcaaaaccgt tctcgatttg gcggattcag atatccggtt tatccgtaaa
    11641 cattttaatg tcgtgctcga aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa
    11701 ctggaagagt ttgcaccgac gaagcaggaa attatctgtt cccgctcgtt tggtgaacgc
    11761 atcacggatt atccgtcgat gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa
    11821 aaacttcgca gcgagcatca atattgtcgg tttatctcca cgtttattaa gacgtcacca
    11881 tttgcgttca atgaacctta ttacggcaat agcgcatcgg taaaactgct gacgcccact
    11941 caggacagca gggatatcat taacgccgct acgcgatctc tggatgccat ctggcaagcg
    12001 ggccatcgct accaaaaagc gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg
    12061 cagctcaatt tattcgatga caacgcaccg cgccccggga gtgagcaatt gatggcggta
    12121 atggatacgc tgaatgctaa agagggcaga ggaacactct attttgccgg gcaggggatc
    12181 cagcaacaat ggcagatgaa gcgtgccatg ctttcaccat gttatacaac gcgaagttct
    12241 gatttactgc gggtcaaata aacatagcgg caggaaaaag cgctcccgca ggagcgccga
    12301 atggattagc gaccgaacag gtcacgtttt ttcgctttaa acggctggga aatcaccacc
    12361 agcaccgcga caatcaggta agcgataaaa ataccgagca gccactgcgg catttccaga
    12421 cctaaaaatt cccactgacg ctcggcgcaa tcgccagagg cgacaaacac ttgcggcacc
    12481 catttatcca gcggcagcca ttccgggaaa cgagccataa aatcacaggt cgcaaacggc
    12541 gaaggataga gctgaagcat ggtgtgctcg taagttaact gcacaccgcg gaacgcacta
    12601 tacaaccaga taaccatcgc tacataacgc agcggagttt tcggggcgat cgcgccaatc
    12661 agcgcagcac ccagaacgcc gaataatgcg cagcgttcat aaatacagag cacgcaaggt
    12721 ttcagtaaca tcacatgctg gaaccacagt gccgtcagtt ccagtgctag agcggtaaac
    12781 gccatcaaca gccacgcgcc ccggccttgt gaacattggt tcaaaaatcg caacataatc
    12841 atttccctgc aatatgcata gagtgcgcag tttaaaccaa ttcattcgct gcgccaccag
    12901 gaggcagcaa aaataggacg caattggaca attatcgggc gaaaaggcaa accgggcatt
    12961 tatatgcccg gtaagttgtt atcaaagcgt tgctatccag cccatttgca taaaccattc
    13021 ggttacaggg gcaagcgtaa actcgacgca gagcaagccg acgagtgtca ggacgagggt
    13081 gtaaggcagg gccatccaca ccatgcggcc ataagagagg cgaatcaatg gcgcgagtgc
    13141 agaggtcagc aggaacagga acgcagcctg accgttcggc gtagcgacgg agggcagatt
    13201 ggtaccggta ttaatggcga ccgccagcag ctcgtattgc ttcaacgtga tagcgccact
    13261 ttccattgcc gctttcgctt cgttgatata aatcgtcccc acgaagacgt tatccgaaat
    13321 ggatgacagc agaccgttga aaatatagaa cagcgacagc tgagcatgtt ccgatgcctg
    13381 caacacaaac tgaataatcg gcgaaaacag ttgttggtcg ataatcaccg cgactaccga
    13441 gaaaaacacc gtcaacagtg cggtgaatgg cagagactcg gtgaaggctt taccgatagc
    13501 atgttcatcg gtgacaccgg tcaatgatgt cgccagaata atgaccgaca aaccaatcaa
    13561 gccaacttcc gccagatgca acgccagcgc agtcaccagc cagacgccaa taatcgcctg
    13621 gacaatcaga cggattttat cctgacgggt acgctggtgg cggctttgat cgtcaaactg
    13681 ttgcagtact tcgcggactt tctccggcag cgtttcacca tagccaaacc aacgcagctt
    13741 ctctaccagc aggcaggtta acaggccaca aatcagaacc ggaacggtca ctggcgacat
    13801 gcgcaggaag aaatcgccaa aatgccagcc agccgcttta gcgatgatca ggttctgtgg
    13861 ttcgcccacc atggtcatca cgccgcctaa tgcggtgccg acaccggcat gcatcatcag
    13921 gctacgcaga aagccacgga actgttccag aaccactttg taatgcttgt cgatatgact
    13981 atcgtcttgc aggtcggtat cttcggtacg ggaagaggct acgcggtgat aaataccata
    14041 aaaaccgact gcaacgctga tcaccaccgc cacgacggtt aaggcatcga ggaacgcgga
    14101 gaggaacgcc gccgccacgc aaaaagagag cgacagcagc attttggagc gaatgcttaa
    14161 cagcaaacgg gtaaatatga acagcaatag ctgtttcata aaatagatac ccgccaccat
    14221 aaacatcagt aacagcaaga cttcaagatt tgccgccacc tcttcacgga cgtgttccgc
    14281 gctggtcatg ccgatgaata ccgcttcgat agccaacaga ccaccgggga gcagcgggta
    14341 gcatttcagg gccatcgcca gagtgaaaat aaattccgct accagcaacc agccagcgac
    14401 gaaagggctg atgaggaaaa ttaacggatt tacgattaag aaaatgatga gggcgagttt
    14461 gtaccagtcg ggggactggc ccaaaaaatt gcgccatagt gcgcggcccc aggagatctc
    14521 catgatggtt tcccttacct tagaaataat caatgatgtt tttatgttta aacgcaaagc
    14581 ttaacggtca gacaggagtg aggcaagtct tgatagtcaa ggggaaagag atgcagaaaa
    14641 tgaagccttg atcccttttt tcttcttttt gtcttctatc agcatagtta gccctctggt
    14701 atgatgagtc caactttgtt ttgctgtgtt atggaaatct cactatggtc attaaggcgc
    14761 aaagcccggc gggtttcgcg gaagagtaca ttattgaaag tatctggaat aaccgcttcc
    14821 ctcccgggac tattttgccc gcagaacgtg aactttcaga attaattggc gtaacgcgta
    14881 ctacgttacg tgaagtgtta caacgtctgg cacgagatgg ctggttgacc attcaacacg
    14941 gcaagccgac gaaggtgaat aatttctggg aaacctctgg tttaaatatc cttgaaacac
    15001 tggcgcgact ggaccacgaa agtgtgccgc agcttattga taatttgctg tcggtgcgta
    15061 ccaatatttc aactattttt attcgcaccg cgtttcgtca acatcccgat aaagcgcagg
    15121 aagtgctggc aaccgctaat gaagtggccg atcacgccga cgcctttgcc gagctggatt
    15181 acaacatttt ccgcggcctg gcgtttgctt ccggcaaccc gatttacggt ctgatcctta
    15241 acgggatgaa agggctgtat acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc
    15301 gcagtctggc gctgggcttc tatcacaaac tgtcggcgtt gtgcagtgaa ggcgcacacg
    15361 atcaggtata cgaaacagtg cgtcgctatg ggcatgagag tggcgagatt tggcaccgga
    15421 tgcagaaaaa tctgccgggt gatttagcca ttcagggacg ataatccctt ccgtttaaaa
    15481 agcaaacccc tcaaacgagg ggtttttcgt tgtttttaca gatttcccat tctcggcggg
    15541 caacgttcca gcaactcgac gctgccgtct tcgttttgct gttccagcat cacatcaaat
    15601 ccccacaggc gatgcacatg cttcagcact tctttgcgcc cccgatccag cggtgcgcga
    15661 ttatgtggaa tgtaacgcag cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc
    15721 tgaatattcg gctccagatt acttaaatta tattgagacg ataaccggtt acggatctcc
    15781 cgataacctt cttcattatg aatagcggaa atctccagat aattatgccg atcgtcatcc
    15841 agcacggtga agaagcggaa atcacgcatc actttcggtg acaggaactg gctgataaaa
    15901 ctctcatctt tgaaatcacg catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg
    15961 atatccggga accagtattt gtcttcttcc gttggcgact gacaaatccg tttaatatcc
    16021 tggaacatgg cgaacccgag ggcatatggg ttgatgccgc tgtaccacgg gctgttatag
    16081 ggcggttgga agaccacatt ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt
    16141 actttccctt catcatacag atggttaagg atggtgtagt gccagaaggt cgcccagcct
    16201 tcgttcatca cctgagtctg tttttgcgga taaaaatact ggctcacctt acgcacaata
    16261 cgcaggattt cacgctgcca tgattccagc agtggcgcat ttttctccat aaaatagagc
    16321 aggttttctt gtggttcgga cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg
    16381 cgcttcggca gggtacgcca gagcatattg acctgacttt gcagatactc ttcgcgactc
    16441 ttctgccggg ctttctcttc ttgcagcgag attttttgcg ggcgtttgta ccggtccacg
    16501 ccgtagttca tcagcgcgtg gcacgagtcc agaagccgtt ctacttcatc aacgccataa
    16561 cgttcttcgc actcggtaat atatttacgg gcaaaaatca gataatcgac aatcgaactg
    16621 gcgtcggtcc agctacggaa taagtaattg tttttgaaga aagagttatg cccatagcag
    16681 gcatgagcca tcaccagcgc ttgcatggta atggtgttct cttccatcag gtaagcgata
    16741 cacgggttag agttaatgac gatttcatag gccagtcctt gctgaccgtg cttatacagc
    16801 cgttcagtct cgataaactt tttaccgaat gaccagtgcg gatagttaat tggcatgccg
    16861 acgctggagt aggcatccat catctgttct gaggttatca cttcaatctg gtgcgggtag
    16921 gtatccagcc ggtagagttt cgccacccgg tctatctctg ccagataaac atccagcagg
    16981 tcgaacgtcc agtcgggtcc atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc
    17041 gtcgccatac gcgcacctca ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag
    17101 catagaacac ctgttaaaaa ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt
    17161 atcagagtgc cactaatccg cttctgaacg gaattttatg ctggataaaa agggcgttca
    17221 gcaggagata ctaaagacgc catattgccg cagagtcagg gagatgtgag ccagctcacc
    17281 ataaaaaagc cgtatgttga ataatatttt caactgagtt atcaagatgt aattagatta
    17341 ttattctttt actgtatcta ccgttatcgg agtggctatg cgagttgtca tactgggaag
    17401 tggtgtggta ggcgttgcca gcgcctggta cttaaatcag gcaggacatg aggtcaccgt
    17461 cattgatcgg gagccggggg ccgccctgga aaccagtgcc gcgaatgccg ggcaaatctc
    17521 ccccggatat gctgcaccgt gggcggcacc aggtgtgcct ttaaaagcga ttaaatggat
    17581 gttccagcgc catgcgccgc tggcggttcg tctcgacggt acgcagttcc agttgaaatg
    17641 gatgtggcaa atgttacgta actgcgacac cagccactac atggaaaaca aagggcggat
    17701 ggtacgtctg gcggaataca gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat
    17761 tcagtatgaa gggcgtcagg gtgggacgct gcaactgttc cgtaccgaac aacagtatga
    17821 aaacgcgacc cgcgatatcg ccgtgctgga agatgccggc gtaccgtatc agctgctgga
    17881 atccagccgc ctggcggaag tggagcccgc gctggcagaa gtggcgcaca aactgacggg
    17941 cggcctgcaa ctgcccaatg atgaaaccgg agactgccag ctgtttaccc agaatctggc
    18001 gcggatggcg gagcaggcgg gggttaaatt ccgctttaat acgcccgttg accaactgct
    18061 ttgcgacggc gagcaaatct acggcgtgaa gtgtggtgat gaagtgatta aggccgatgc
    18121 gtatgtgatg gcgtttggtt cttactcgac ggcgatgctc aaaggcattg ttgatattcc
    18181 ggtgtatccg ctgaaaggct actcgctgac cattccaatt gcgcaggaag atggtgcgcc
    18241 ggtatccact attcttgatg aaacctacaa aatcgccatt acccgtttcg ataaccgcat
    18301 tcgtgtcggc ggaatggcgg aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg
    18361 tgaaacgctg gagatggtgg ttcgcgatct ctatccacgc ggcggtcatg ttgagcaggc
    18421 gactttctgg actggcctgc gcccgatgac gccagacggc acgccggttg tcgggcgtac
    18481 acgctttaaa aatctgtggc tgaataccgg tcacggcacg ctcggctgga cgatggcttg
    18541 cggttccggt cagttgttaa gcgatctgct ctcggggcgc acgccagcga tcccgtatga
    18601 agatctaagc gtagcgcgct acagccgtgg atttacgcca tcacgcccgg gccatttaca
    18661 tggcgcacac agctaaggaa acgagatgac ccgtccgata caggccagcc tcgatctgca
    18721 ggcattaaaa cagaatctgt ccattgtccg ccaggctgcg ccgtacgcgc gcgtctggtc
    18781 ggtggtaaaa gcaaacgctt acgggcacgg tattgagcgt atctggagcg cgctcggggc
    18841 caccgatggc tttgcattgc ttaatctgga agaggcaata acgttacgtg agcgcggctg
    18901 gaaaggaccg atcctgatgc tggaaggatt tttccatgct caggatctgg agatttatga
    18961 ccagcaccgc ctgaccacct gcgtacacag caactggcag ctcaaagcac tgcaaaatgc
    19021 gcggctaaaa gcaccgttgg atatttatct taaagtgaac agtgggatga atcggttggg
    19081 cttccagccc gatcgcgtgc ttaccgtctg gcagcagttg cgggcgatgg cgaatgttgg
    19141 cgaaatgacc ctgatgtcgc attttgccga ggcggaacat cctgatggaa tttccagcgc
    19201 gatggcgcgt attgagcagg cggcggaagg gctggagtgt cggcgttcgt tgtccaattc
    19261 ggcggcgact ctgtggcacc cggaagcgca ttttgactgg gttcggcctg gcattatttt
    19321 gtatggcgct tcgccgtccg gtcagtggcg tgatatcgcc aataccggat tacgtccggt
    19381 gatgacgcta agcagtgaga ttattggtgt ccagacgcta aaagcgggcg agcgtgtggg
    19441 ctacggcggt cgctatactg cgcgcgatga acagcgaatc ggcattgtcg ccgcaggata
    19501 cgccgacggt tatccgcgcc atgcgcctac cggtactcct gttttagtgg acggcgtgcg
    19561 caccatgacg gtggggaccg tctcgatgga tatgctggcg gttgatttaa cgccttgccc
    19621 gcaggcgggg attggtacgc cggttgagct gtggggcaag gagatcaaaa ttgatgatgt
    19681 cgccgccgct gccggaacgg tgggctatga gttgatgtgc gcgctggcgt tacgtgtccc
    19741 ggttgtgaca gtgtaacttg ttatcgctgg atgcgaccag cgtcgtatcc agcgttacaa
    19801 aacacattaa acttaagatt cggcttcttc ctccgctacc cgaacaccaa tcttcaacac
    19861 ttcattatcc tctttctcgg ccaccgtcca gatcattccg gcaaattcta cctgatcacc
    19921 gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt gctgcttatc
    19981 acgatattct cgcccgtctt ccaggccata tatcagcgcc acatcagcat atttggcact
    20041 ggcttcgaga atgaagtcac caaagaagcg ttgatctagc gcgaccggtg gcgactggct
    20101 gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca acacatcgcc
    20161 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagtgccgc
    20221 aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcgcccacgc accatttatc
    20281 ggcactcaac tgataaacaa actgctccca cggattttcc ggatgaatat ccagaccaac
    20341 gcgtgacacc ggacgtccca ctggcggaac gaccactttg gcttttttcg ccgcccacga
    20401 gagtgatgtc ccctgcaaga gtagtgaaac cagaaccaca aagaaggcga cattaaagaa
    20461 cagtcgcgca ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc
    20521 gccgcgtaat cccacccagc tgataaacac gcgctcacgc agattgaagc cacggaacgg
    20581 tagcaatccg gcaaataccg aaagaggacg ggcgaagaaa atcatccatg cggacaaaat
    20641 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaacagcc ccagcaccag
    20701 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagaa tgccgtagcg
    20761 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
    20821 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
    20881 atataaaccg gcgggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
    20941 aagaccgatg acaatcccga ggccaaattg ctgcaaaata tcgaccacga acatccagct
    21001 gacactgctt tcatgctgct ggatcatcgc aatcagggta atcgtcagaa agaccgccat
    21061 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
    21121 accacccagc agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa
    21181 taatccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
    21241 ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca cacgaaagga
    21301 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca gattacttac
    21361 catgtaggcg aagggataat tatcaaacgg gataccgccg acgccatcaa cccccgccag
    21421 catgccgatc gccagaaaaa taaccagaat gggaatgcca agacgggaag aaaatgaact
    21481 aagtaaaata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
    21541 ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
    21601 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
    21661 gatgaccgct aatcgtcaac tgggtgcctt cctgggtgtt attcagaatg gcatgtgcgc
    21721 ccaacggcag tgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
    21781 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
    21841 cattaggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
    21901 cagcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt
    21961 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
    22021 gcatcgcgag attgcctccc cacagcgtgc cttcggtctg acatgtcggg ccttcccctt
    22081 gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg
    22141 cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
    22201 tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagca
    22261 acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac
    22321 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
    22381 gagaattgag atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg
    22441 caataacctc gacgttattg acctgatgcc ccgcgtcggt aaggcgctgg ataccacgca
    22501 gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac agagacatgg
    22561 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
    22621 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
    22681 tttgattgtg ttattagcgg gttgttcatc gaagcatgac tatacgaacc cgccgtggaa
    22741 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
    22801 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
    22861 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
    22921 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
    22981 aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
    23041 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catatgctaa
    23101 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
    23161 caacgatctg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
    23221 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
    23281 actttgtccg ctttctcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
    23341 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
    23401 tcattcttgc catcaatcac tttgcgctcg ctgatggaga ttaactgggc gtcaaagtga
    23461 aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc ttcgtgcaat
    23521 ccggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacagg
    23581 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaatatgg cggatggagt
    23641 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc acaaggtggg aggcggtacg
    23701 gtaataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
    23761 actttcgcac cctgggtttc ggcggtaatg gtaatgtgct gcgcctttaa cacagcgttg
    23821 ttatcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
    23881 ttgctgatca gctgcccgcc attccaactg atacgcaaag gaattgcggc tttgtgcaaa
    23941 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
    24001 ctcacgcgga aaaactcctg actgacaaac tgtcttaggt ttatcggtca cagttaacaa
    24061 aacttaatac aaatgcgtga atatttttta catgtcgttc ttaaatcagc cgacatacgc
    24121 ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgtcc
    24181 gattcatcga aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat
    24241 agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
    24301 ctgtattctt gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
    24361 tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtag tgggcgctat
    24421 cctggtgctg ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag
    24481 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgatacc tgcccagaaa
    24541 ttgcgacctt cttcaataaa cccttcactg taggcatatt tggtatcaaa caggttattg
    24601 accgacgcat tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc
    24661 gcaaaaccgg cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct
    24721 tccgacagcg ttacgcttaa cggctcccac ggtttgagag tcatccatgc ggtcattgtc
    24781 tgcgttggca gatcggttat cttgccgatg tctttgcgtt tggcatcagc gtggatcagg
    24841 gcgtagctca atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga
    24901 ccgctgtaat ccaccgtgcc gctgttctga ttttgaatgg tatcggcatc gatattgtgc
    24961 gagaggatgg catcactcac ccggttatag taaacgctga cctcaaaccc ccagtcgtgc
    25021 gtgaaggcac cattccaggt taaatccacc ccgcgcgcgc gttccggttt gagctgcggg
    25081 ttaacaatcg ctatctggtt atacgcaggt ttggacgtgg tatagcgttc tttcagcgtc
    25141 ggaaagcgtg tgcggtcata gtacgaaagc gccagcgtgt cttcattggc aaagtggtat
    25201 ttccccatca cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctt
    25261 ccatctttct catgtttttt cgcttctacg ctatcgcgcc agtcatagct gattccagcc
    25321 acgacatcga cattatcggc agcagcccat tgatattcac tggcgagcga ccaggtacga
    25381 tcttcatagc gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc
    25441 cagttaacgg caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaacccg
    25501 gcaccgtcgc tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc
    25561 agcgagttgt acatcatcag cgtattttca aaggtatcgc gatacagccg acttttcagg
    25621 gtaaaacgat cgtttagttg ggtcgttccc tgataataaa aactttcttt gtcatactct
    25681 ggccactgcc agtagcgtga tttttgacca ctatttccac tgtatggcgg attatctttt
    25741 tcaccatcct gcttaatgta agtcaatgtg tattcatcgt tttcgcgtgg tgtaaaaccg
    25801 agcttcacaa tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct
    25861 gcaatatcat tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg
    25921 ctgacttgca aataccccag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca
    25981 ttgtcctggc tacggctcca tccctggcga tatcccagat ttgcttccag aggttttgtt
    26041 ggcttctggg tggtgatatt aatggctccg cccatctgat taggcccctg aagcagcgac
    26101 gaataccctt tggaaacttc aactgccccc aaattgttgg tcagaatccg cgccagatcg
    26161 aggttgccgt catagggaac ataaatgggc acaccgtcga aatagactgg cacctgacga
    26221 ctatcaaagc cacgaacttt gacctgttct tcgttgcggc tacctgactt ttgcagcacc
    26281 acgccgggga cgacacttaa tgcctgagcg acattctgtt tatccagggc cttcatggtg
    26341 gggtgatcga gaacggtggt cgtcgtcgat gataccggac tggaccatac ggtcagcgtg
    26401 tcactctcct gagccgctaa ggcctgctgg gtaaaggcaa gtgataatgc tgtgcagaga
    26461 taacgttttt tgagtctcat agaataaatt ccgtttaaca aagaattaaa tagaggctgc
    26521 gggttgcaga ctgactttaa tgtcagctgg tgtcgcgtaa taaggcgcgg aggtaataaa
    26581 aaggcgaatg ccgcagtcga ggtaattttt gagtgttgtc agattaattc cgccggtcag
    26641 agccagcgtg cagtgaggag ccagcgacgg tgctatttgc gcaatttcag ttgcctgttg
    26701 cggattaaat ttgtcgagct gaagtacgtc tggttgcgcg cgtaacgcgg cgattgcctc
    26761 tttcggcgtg tcggcttcaa caactatttt cttctcgggt gcgtggcggc gtaactgatt
    26821 gattgcgcct gaccagtcct gattgtcatg aagaaaatga cggtggttgg caaacaataa
    26881 tatggtttcc gcacatccgg cacgatgaat cagtcctccg gcagccagaa ttgcctgcga
    26941 ggccagtaag cgggtgcccg gaattgcttt tcgggtgcag gcgatattgc catcagggta
    27001 acgttcacga agtaacgcca gcatttgagc gagataatca gaaacaccgc aactccactc
    27061 cagcacattc tggactgcct tccatccctg atgaagtgct gcggcattac cctgcgcacg
    27121 gattagccgc tgacccgcgt tcgcttgtga accgtcgctg actgcgtcat caatagttaa
    27181 tcccagcgta gttaacatct tacacgcgac agaaattccg ctgacgcagc cgccctgacg
    27241 gtgaaaaaac tctatatagc catgctggtg tccaatattt aatgctcgcg tggtcaggtc
    27301 accgccctgg atatcttcca gaagtaacgc atcgatttgt gcctgagaaa ggaaaatcat
    27361 cgcagtgcct cttgcggtac gctatcccac cagatgagcg cccagtcgcg gctggcaggg
    27421 gcgagggcac gagcatcttg ttgttgatac cagcgatata accgctcacg ctcatcgtcg
    27481 ttaagcgcgc ccagactcca gctgacgttc tgctcaaaac gttcccaggt gctgttatcc
    27541 tgttggcaat tttgtccgcg gataaaatca acgtgagcat aaattcccat ctgatacagg
    27601 acgttgagcg caaagatata gttgggtaat tcgaccactt cccgaccagc cgcccgctga
    27661 atggctggag agacgaacga ggtgctgacc agatgagtcg tatagacgcg aagccgtgcc
    27721 tgattattca gtttgctcat tgcctggcgc atatcagcca ccagagtgga gcgggaagcc
    27781 acggcaatat cgcagcgtgg caggtcactc cagtcctctt cccaggcgcg ctggatccag
    27841 tggacattgt ctgcctttag cgcggcggcg cgacgggcgg caacgttcag catcccctga
    27901 ctgtaatcaa cgccataaat cgtcgtgagt ttgtccgcca gcgccagcga tacggtaccc
    27961 ggtccgcagc ccatatcgaa taaggtttgc gcgccttgca ggtcaatttt tgaggtcagt
    28021 tgttgcaggt agctgtccgt cggcgaggcg caattttccg ccattttttc cgcccgttga
    28081 tcccagtgat ccggcgtttt ttctgtgcga tgggccagcc tgagttgctg aaggtaaagg
    28141 tctgcaaaat caatatcgtc aatgagcatg atttttcctt agtgcttaat tgcagagagg
    28201 tgatggtgaa tctggtcggc agaaacccgg tacagggcag ccagtttttt cgttgttaac
    28261 tgttcggtcg gtaagccttg tgttactcgt ccatctggct ctacctggat aatgctgtcg
    28321 gcaatggcgt ttgcatggag cggatgatga gtcgacatta acattgtcat gccgttattt
    28381 ttgagctgcg ccagcgtgtc cagaagttgg atctgatgac cgaaatcgag gctagaggca
    28441 ggctcatcca gtaataacaa gcgcggttgc tggacgagtg cacgggcgat taaagccagc
    28501 tgacgttcac cgccgctaag cgtattccag cgtcgtgtcg ccagatgcaa aatgcccagc
    28561 ttttccagtt gctccgttgc tttcaggcgt tcttgcttac cgggaacgga aaaagcgcct
    28621 atggtggggg cctggcccat caacaccata tccagcacac taaaagcaaa aatgccgtcg
    28681 tgcgcctgcg gcacccaggc aatagcctgc gcgcgctggc gatggctgag tgtctgcaca
    28741 gggaggctat ccagtaagat ctcgcctttt aataacggta taacgccaag aatggagcgc
    28801 atgagtgtgg ttttgccgca accattggct cctaacagac aacaaattgt tccttgcgac
    28861 agggtgaagg acacgtcgcg tagcaccgga tggcgggagt aacccagaga cgcatgacgg
    28921 acggcaagca aagtcactgg cgacctccgc gtaacaacaa aaacaggaaa aacggtgcgc
    28981 caacaaaagc ggtcagaatg ccaagtggga tttctgttgt tccaatgctg cgtgccagcg
    29041 tatcggtaag caggagcaga atcgcgccag tacacattgc catgggcaac agctgctgat
    29101 ggttatgacc ggtcaacaag cgggcaatgt gcggaacgac caggccgacc caaccaatta
    29161 tgcccgctat tgctaccgtg ctggcggtga tgagtgtggc acagacaatc aatccaaagc
    29221 gcagacgtgt cacgttcagt cccagcgaac gggcttcgtc gtcggaaagc gtgagtaaat
    29281 tcatgcgcca gcggagaaaa aacaggggca aggagcctat gagaatgatc ggtgcagcat
    29341 agcagagatc gcgtaaggtc acggtggaca gcccccccag caaccagaag gtaattgacg
    29401 gtaactgggt ataaggatcg gccagcgttt ttattagcga tatccccgct ccgcacaacg
    29461 ttcctaacgc aatgcctacc agcaccaggg ttaagatcgg atcgtggcgg gtcacccgac
    29521 gcgtaatcaa ccagacgccc gcgaccacca tcagcccgcc gcaaaatgcg tacagctgga
    29581 tgtaaacaat ggataatccc cacaggattg ccgcgcaggc accaagtccg gcaccggcgg
    29641 agacgcctaa aatatccggt gacactaagg gattacggaa cattccctga taggtggttc
    29701 cggcccctgc cagcgccgcg cccagaaaca gcgcagcaag aatacgcggc acgcggattt
    29761 gccagaagac aatttgttcc tgcacagtaa cattttcttg acgaagaatg agcgccagca
    29821 gttgctgcat atcgagatga taagccccac ttacggcggc aaaagtgata ctgatagctg
    29881 ccagcagcaa aatacatccg tttacggttc tcattagctc gccaccaatt tttgatagtc
    29941 ggcgtcactt aatgggcaat gccagaacag ttgggcgtaa tgctgcatgt cacttttaaa
    30001 ctggcgattg atggcgggat ccagccaggc gtgaagtctg cgtaatccca gaagacggtt
    30061 gattcccggc ggggcatcca gccagccaaa gggcaggcca cttaaaaata agatgcgttg
    30121 ttccgcaacc gctttcacgc cctgccagag aggatcacga cgaataaaat ctgcagtaac
    30181 ggcctcctga accagaataa tatccggctg ccagcgcagg agattttcca tggaaacctg
    30241 tgtcagaccg tggcgatcgg ctatttgcgc gacgttgtgc aaacctaata attccgccgc
    30301 ctcggtatgc aacgaaccct gtaagcccgt ttccagccct cgaggaccgc gcgcagcata
    30361 aaagctgaga ttagcggcgg gtgaggtggc gaatgcctga gcttcaccaa cgaagcggct
    30421 ggcgagattg gcttgttctg cggcgcggtg ctcttcgcct aacgttttgc caagcgttgt
    30481 taactgttct gctgattgtt ccagtttccc gttaagcaat aaccagggta tttgtgtctg
    30541 ttcgctaacc tgccgtgcct gggagatcca ggtttcatcc gtattgccgc aatcaacgac
    30601 caaatcggga tgtaacgcca taagcccttc cagcgagagt gtactggcgc gcccggcgag
    30661 tcgtcccagc ctggggaact ggcgaatgtg ctctggcaat ggaattaatg cctgacgggc
    30721 aaaatcaaaa gaggaaaaac cgaccatttt ttctggcgcg acagccagca atagtaaatc
    30781 ggcgggcgcg cctgcgctga cgatgcgtgt aatgttttgg gcttcgggaa gcgtcagtga
    30841 ggcccagaat ttgactgatt gtgcaaagga cggcagatga tgcaggaaca atgtcgacgc
    30901 aagaatctgt gtaaacctcc gtctggttag tgacataagt aatatcctga taattaaaac
    30961 cgctatatac ttacacatat agcgaaaaag atcatgtttg atgtcaaaat ttgtctaaag
    31021 agacggaatg atttgcttgt tgcttaaata acactttgtt ccacaagaaa attgtcaggg
    31081 agaatgggga gtgggggaaa atataaatag aggtgtaatg attaacaagt tgataaaata
    31141 aaattaaaat cttctctaat aaaagaaaat aaaggcatag accgtagaaa gagtggtatt
    31201 tggtatttcg tggtgaagaa agagcaatgc caattttatg cacgtaatgg cattgctcga
    31261 tatacagtaa agttaaggcg taggttccgc ctcattttgt tttaccggct gtgttgttga
    31321 ttcactgagt ggacgcgtcg ccggaacatt gtcgcacggt tgctctttcg ggcagatcaa
    31381 atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc
    31441 gccaccgccg ccccctgttc cggtggtgct gacatcatat ttttccacca gctttttctc
    31501 ccggtcatag gtgtgctgaa cattggtcag gaagtgccag ctaatgtcca tcgccacctc
    31561 tttttgcccg tagttttgta atccttctgt cgcgacccac tgcaacggtg cccagccgtt
    31621 tggcgcatcc cattgttgcc cacttttcac cgacgtggtg ttcaggccgc ctggttgcag
    31681 cagatgtgtt ttcgtcgccg tcgccatttt gctggcgcga tctttcgctg ccgcattgac
    31741 gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc actttatgac ttttcaggtc
    31801 gtaatcggca taccagcctt gttgatcgtt ccacaggtat ttttcgatcc ctttttgacg
    31861 ggcattcgcc agcgtttcgt actggtttgc catcgcgtta tctccggcag ctttgctggc
    31921 gcgggcgagg attttttcca ttttaaacat caggctgttc agatcgaccg gtacgatgct
    31981 ggtggtgcgt aaggtattta actgctgcgg attgtccatc cagcgcgagc tgaaatccca
    32041 gccagacgca gcggcagagc gcaggtcgcg gtaaatttca gtggcaggtc gattcggatt
    32101 gcttttggcg gtggcaatat cttccaccca tgactctggt cgtggcgtat cgcgatcgtc
    32161 ccagtagcgg ttgagaaggg taccatcctg aagtttgaca acgcgttttt cctgttgtcc
    32221 ggcttgcagg ttttcaacac cgtccatcca gtaagcatat tctttttgca tttgcggcag
    32281 gtattgcttc aacgcggcat cgccttcatg ctgcgccagt aactctacca tcagggcaaa
    32341 gaatggcggt tgtgagcggc ttaaatagta actgcggttg ccgttgggaa tatgcccgta
    32401 agtgtctatt tcatgagcaa aattggccac catatccgcg actttatccc agtgaccact
    32461 ttcggcaagc cctaacatgg tgaagtaact gtcccagtaa tatacctcgc gaaaacgtcc
    32521 gcccggcaca acataaggtt ccggcagcgg taacagagaa tcccattttt cggtattttc
    32581 ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt tcgcgtagtg attgcccctc
    32641 tggcggaaca tatttctcgc cctctttcgg cagggtgaaa ttgacgttaa cgaaatggcg
    32701 tagatcaaat ccgctctggt tttgctgcat ccgataatca gcaaggatca tcagcggatc
    32761 gctgttcggc acggcatcgg caaaggtttt ttggtccgga aaaagtttgg cgttttgcac
    32821 atcattaaac agcggcccta ataaaatatc aggcggctgt ggcgttaccg gtgtttcttc
    32881 tgcctgcacc gatagcgcag cgaaacacaa aaagatgcag gctggaatta acgccatttt
    32941 tagcgggcga gaagctgcgg gggatttcat caatcattct cctttggcga aaccgaataa
    33001 aacggtattc aagtatcaga aaaccttagt tcaggatcgc actagctgca tgatcgaaat
    33061 ctcattctgc gaacggtcag gcattttaga aaagggaatt aaagggataa ataacgtcga
    33121 aatagagtga tcaaataacg tctgtttgtt agccagccag cggcgttggg ttcgcgatta
    33181 gcgcagaaaa aatcatcatg atttggtaaa aaatataaca ggtagttaaa aatcatccgt
    33241 gcagagtaaa tagccggatg gcatcagaac gatgccatcc gaacggtggt ttaaccctga
    33301 cggctgaaac gttgcgtttt aacgtccagc gttagcgttt cttctggttg tatcgcatac
    33361 agtttctcac cctgctggca aatccagcca atccccagtt cacgggcgat cagggcgctg
    33421 tgggatagcg gacttccggc gctaaggcag atacctttta caaccgccgg atccagttgc
    33481 agtattgtgg agggatagat gttctccgcc agtagaatag ttggcgagtt aaactgcggg
    33541 agttcttctt tcgtttgggt caggtggacc agggtgcgat gcagaagatc gtccacatca
    33601 atatagcgag cctgtagata ttcatcatcc agttgctggt attgctggct aagttcttta
    33661 agaacttgct gccaggcata ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc
    33721 gccgccagca gttccggatc atctaacagt gtatggtgac cagaaaagat tgcggcaata
    33781 tcgtcaagcc cgctggcttc tgctttcgct gttaacgtca tcagatctaa taacgtgaag
    33841 tcaatggcct ggcgtaatcg ttcttgttct tcatccacgg tcagggttga ttttgcctgt
    33901 accgtacata aaaccggttg ataataaaag gctttacccg aaacaggcgg aacgggacgc
    33961 agaataggtg gagcaacttc ctccgtttca ccaaagttat cttcagccag ctgacggaaa
    34021 gcgatcagtg cctcttcagc ttctggccct ttcgcaatca ggcgcagcgt atcgttatag
    34081 cgaacttgta gtaacgcaat ctggttaata ctctccggtg tgacgcattt gccgtttttc
    34141 tccagcaaca tatcggcatt aaatgtcgat aaggtataaa ccagccggga ggccggacgt
    34201 acatgcaggc cgttacggtt ttttatgacc accgccagag aacgggcttc ttcatcgtac
    34261 gaaggacatg tgtcagagat ttcagtgttg gaggagggta aacccagttg ttcacgtttg
    34321 gcttccagcg catgcatggc gtcaaagatc actttgtcga tctccgcccc cgaggccgcg
    34381 ctaaccgttg ctgccagtgt accttcgacc aacggcgcag cacacaaacg tacttttgcg
    34441 gcgatctcgg gagccagcaa ttccagcgca gtttcagcac tcaataatgc gcttcctata
    34501 tccatcatga ccagcacatg gtcggtatcg gcaacagatt cgatggcctc catcactttg
    34561 acggcatcgg taccaatggg attttgtgga tcgtcaattc ccgcggcaat ggcgatttta
    34621 caattatcac tcattaacat ctgacgggct aattcaccga cgccttctcc cagtcggctg
    34681 ctatgtgaaa ctatgaccag gtttaccatc accaattcct tactcttttg cggctaacgc
    34741 caacatttgc atcataaaca tcaccgaggt cgcaccgggg tcctggtgac caatactgcg
    34801 ttcaccgaga taactggcac ggcctttgcg agcttgcatc gtaatcgtac tttgtgcagc
    34861 ggattcggcg acgctactgg cagcttcgag cgccgccgga atagagagat tttgctcgct
    34921 ggactgacgt aacgattcca ccaccggcac ccacacatca cacatggttt tatcgccagg
    34981 ttcggctttc ccgcgactga ttacgccgtc cgcgccatcg cggaacatct gataaagctc
    35041 ttccagcgtc aggctttgcc gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc
    35101 gaaaagcgga ccactggcac caccgacgct ggaaagtagc gtcataccgg tattcttgag
    35161 aatgaaaccg atatctttat ctgcgatagc agggagtttt tccaccactt tgctaaagcc
    35221 tcggttcata tttagcccgt ggtcagcgtc gccaatttcg cgatccagtc cggtaagata
    35281 ctcgctctcg gtgctgaaaa tatcgccaca acgagtgagc cagttaacaa tttgagttct
    35341 gctcagtgac attgctatct ccttatttac cccagttaag ggccggggtg tggaccgggg
    35401 cgtcccagag tgccagcgtt tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca
    35461 tatccagtga ggtgcagtac gcgccaatta aattacgctc gatagtcaat cccgcttgct
    35521 ggcaacgtgt ggtcaggcgg ttatagacgc cgtacagctc agaaagcgga gttgcgccaa
    35581 gattgttaac cagcgcaatc acccgatcgc cagactggag cggttgttta gtttgtgatt
    35641 cttcctgcca actgccttgt tgataatccc agaaacgcaa agtgcgatgg tatgagccat
    35701 tttccagcag ggtgtcgaac atttcatcga cggtttgatc aagggaagag aagggacggc
    35761 ggtcaatacc tggctcacca tgaatgccga cgccaaactc catctcattg tccgccaggg
    35821 taaaagaagg tttgcccgcg gcaggaacgg tacaggcacc gagagcgata cctattgagt
    35881 ggccttgatt attcaactta cgccccagtt ccgcacaggc gtccagtgag tcgccacgct
    35941 cggccgctgc gcctacgagt ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg
    36001 cggtataaag actgtctttt accgcaacgt cgtcatcaat gaccacagtg gtcactttta
    36061 cgccgctatc gtgcagtaac tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat
    36121 ttttgataat caacagtaca ccttcgccgc catcaatttg catggcgcat tcaaagattt
    36181 tatcgggcgt cggtgaggtg aaaatttcgc ccggacaggc ccccgaaagc atcccctgac
    36241 cgatataacc acaatgcatc ggctcgtgtc cgctgccgcc acccgacagc agggcgactt
    36301 tacctgcaac gggggcatca gatcgggtga catacaccgg atcctggtgc agtgtcagag
    36361 atggatgcgc tttcgctagt cctgccagtt gttcgtccag tacgtcttgc acatcattga
    36421 tcaatttttt cattattttg ctccagcaat tacggtaggg catggatgat gttcaacgac
    36481 acggcgacca ctgactgccg atgaatccat tgtgcatcag gcaagggaaa agaaaattag
    36541 cccaaattat gtttcatagt gaaacatatt ctttaatgaa tgttccatat tgaaactttt
    36601 acgtgtatta atacttaaaa ttgcgagccg gaacaccttt tgtcataagg gatgcgggat
    36661 atgagtagcg cttttaacaa cgatggtcgg gacatatctc ccttaattgc aacctcctgg
    36721 gagcgatgca ttaagctgat gaaacgggat acatggaacg taccacacca ggcccagggc
    36781 gtgacatttg cgtctattta tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg
    36841 ctggaagatg cctgggaata tatggcaccg cgagcgtgtg cgctgcttat cctcgatgaa
    36901 accgcctgca ttctcagccg taatggcgat ccgcaaacct tgcagcaact aagtgcactg
    36961 ggattcaatg acggcacgta ttgcgccgag ggaattattg gtacttgtgc gctatcgtta
    37021 gcggctatct ctggtcaggc cgtgaaaacg atggccgatc aacatttcaa acaggcactc
    37081 tggaactggg ccttttgtgc aacgccgttg tttgacagca agggccgatt gacgggaaca
    37141 atagcgctgg cgtgtccggt tgagcaaact accgcagctg atttgccgtt gacgttggca
    37201 atcgcacgcg aggtcggaaa tttactgctg acggacagtt tgctcgctga aactaaccgt
    37261 catttaaatc aacttaatgc cctgttagaa agtatggatg atggcgtgat tagctgggac
    37321 gagcagggta atttgcaatt tattaatgcc caggcggcgc gggtcttacg ccttgacgcg
    37381 acggcaagtc agggacgggc aatcactgaa ctcttaacgt tacccgccgt attgcaacaa
    37441 gcaataaaac aggcacatcc gctcaaacac gtagaagcaa cctttgaaag ccagcaccag
    37501 tttattgatg cggtgataac ccttaaaccg ataatagaaa cgcagggaac cagctttatt
    37561 ttgttgctcc atcctgtgga acagatgcgg cagttaatga ccagtcaatt aggaaaagtc
    37621 agccatactt tcgctcatat gccacaagag gatccgcaaa cccgccgttt gattcatttt
    37681 ggtcgccagg cggcgcgcag tagctttcct gtcctgcttt gtggagaaga gggcgtgggc
    37741 aaggcactgc taagtcaggc aattcataat gaaagtgagc gtgcagcagg gccttatatc
    37801 gccgtcaatt gtgagttata tggtgatgcg gcgctggcgg aagaatttat tggcggcgat
    37861 cgcacggaca atgaaaatgg tcgtttgagt cgactggaac tggcgcacgg cggcacgctg
    37921 tttcttgaaa agattgaata tctggcggtg gagttacagt ctgctttgct tcaggttatc
    37981 aagcagggtg ttatcacgcg actggatgcg cggcgtttaa tacccattga tgtcaaagtg
    38041 attgccacaa cgaccgcgga cctggcaatg ctggtggaac aaaatcgttt tagtcgccag
    38101 ctgtattacg cgctgcatgc atttgaaatt accatcccgc ctctgcgtat gcggcgtggc
    38161 agcattccgg cgctggtgaa taacaaatta cacagtcttg aaaaacgctt ctctacgcgg
    38221 ctgaaaattg atgacgatgc cctcgctcgc ctggtttcct gtgcatggcc tggcaacgat
    38281 tttgaacttt acagcgtcat cgagaatctt gctctgagta gtgataacgg gcgcattcgc
    38341 gtcagtgatt tacctgaaca tctctttacc gagcaggcga cagatgatgt cagcgccact
    38401 cgcctttcca ccagtctgtc atttgcggaa gttgaaaaag aggcaattat taacgcagcc
    38461 caggtcacag gcggtcgcat tcaggaaatg tcggctttac ttggaatcgg ccgcactacg
    38521 ctgtggcgga aaatgaagca acatggcatt gatgcagggc agtttaagcg ccgtggatag
    38581 cgggagagta tgtactttcc cgctatgaat tgctaattaa tagcgaccat gactgccgtg
    38641 atttggtcca ccatttgaag gtggcattgg cttaccggag ggcgcaggac cacgagggcc
    38701 atcgctgtgg cggtgatgat ggtggggaac acaagcactt aacaggctcg caaagacaat
    38761 tgctaacagg ctgaaggtaa atgttttttt catgacaaac tcttgaacgg ttaatgcact
    38821 gtgattgtta tcacataaaa ggcacattgt taatattgcc aggagtctga ttatgatttt
    38881 cgataactca gaattgaaag aagccgcgta gcagg
//
LOCUS       DAZGTJ010000039        38565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000039 DAZGTJ010000000
VERSION     DAZGTJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.039"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..79)
                     /gene="ylcJ"
                     /locus_tag="ACHK8E_002480"
     CDS             complement(<1..79)
                     /gene="ylcJ"
                     /locus_tag="ACHK8E_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFP0344734.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRK"
     gene            complement(127..459)
                     /gene="emrE"
                     /locus_tag="ACHK8E_002481"
     CDS             complement(127..459)
                     /gene="emrE"
                     /locus_tag="ACHK8E_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP0344735.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(527..829)
                     /locus_tag="ACHK8E_002482"
     CDS             complement(527..829)
                     /locus_tag="ACHK8E_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP0344736.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(826..1527)
                     /locus_tag="ACHK8E_002483"
     CDS             complement(826..1527)
                     /locus_tag="ACHK8E_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0344737.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1524..2543)
                     /locus_tag="ACHK8E_002484"
     CDS             complement(1524..2543)
                     /locus_tag="ACHK8E_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0344738.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2540..3079)
                     /locus_tag="ACHK8E_002485"
     CDS             complement(2540..3079)
                     /locus_tag="ACHK8E_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP0344739.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3149..3379)
                     /locus_tag="ACHK8E_002486"
     CDS             complement(3149..3379)
                     /locus_tag="ACHK8E_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0344740.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3484..4173
                     /locus_tag="ACHK8E_002487"
     CDS             3484..4173
                     /locus_tag="ACHK8E_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0344741.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4769..4975
                     /locus_tag="ACHK8E_002488"
     CDS             4769..4975
                     /locus_tag="ACHK8E_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP0344742.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            5051..5347
                     /locus_tag="ACHK8E_002489"
     CDS             5051..5347
                     /locus_tag="ACHK8E_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0344743.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5353..6138
                     /gene="bet"
                     /locus_tag="ACHK8E_002490"
     CDS             5353..6138
                     /gene="bet"
                     /locus_tag="ACHK8E_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0344744.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6135..6815
                     /locus_tag="ACHK8E_002491"
     CDS             6135..6815
                     /locus_tag="ACHK8E_002491"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0344745.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6812..6994
                     /locus_tag="ACHK8E_002492"
     CDS             6812..6994
                     /locus_tag="ACHK8E_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP0344746.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            6967..7158
                     /locus_tag="ACHK8E_002493"
     CDS             6967..7158
                     /locus_tag="ACHK8E_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0344747.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            7235..7450
                     /locus_tag="ACHK8E_002494"
     CDS             7235..7450
                     /locus_tag="ACHK8E_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0344748.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7549..7758
                     /locus_tag="ACHK8E_002495"
     CDS             7549..7758
                     /locus_tag="ACHK8E_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0344749.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            7815..8093
                     /locus_tag="ACHK8E_002496"
     CDS             7815..8093
                     /locus_tag="ACHK8E_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0344750.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            8065..8130
                     /locus_tag="ACHK8E_002497"
                     /pseudo
     CDS             8065..8130
                     /locus_tag="ACHK8E_002497"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8292..9455
                     /gene="intD"
                     /locus_tag="ACHK8E_002498"
     CDS             8292..9455
                     /gene="intD"
                     /locus_tag="ACHK8E_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP0344751.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9471..9547)
                     /locus_tag="ACHK8E_002499"
     tRNA            complement(9471..9547)
                     /locus_tag="ACHK8E_002499"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9511..9513),aa:Arg,seq:tct)
     gene            9818..10684
                     /gene="folD"
                     /locus_tag="ACHK8E_002500"
     CDS             9818..10684
                     /gene="folD"
                     /locus_tag="ACHK8E_002500"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP0344752.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            10686..10898
                     /gene="ybcJ"
                     /locus_tag="ACHK8E_002501"
     CDS             10686..10898
                     /gene="ybcJ"
                     /locus_tag="ACHK8E_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP0344753.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            11006..11527
                     /gene="ybcI"
                     /locus_tag="ACHK8E_002502"
     CDS             11006..11527
                     /gene="ybcI"
                     /locus_tag="ACHK8E_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0344754.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(11563..12948)
                     /gene="cysS"
                     /locus_tag="ACHK8E_002503"
     CDS             complement(11563..12948)
                     /gene="cysS"
                     /locus_tag="ACHK8E_002503"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0344755.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            13122..13616
                     /gene="ppiB"
                     /locus_tag="ACHK8E_002504"
     CDS             13122..13616
                     /gene="ppiB"
                     /locus_tag="ACHK8E_002504"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP0344756.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13619..14341
                     /gene="lpxH"
                     /locus_tag="ACHK8E_002505"
     CDS             13619..14341
                     /gene="lpxH"
                     /locus_tag="ACHK8E_002505"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP0344757.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            14459..14968
                     /gene="purE"
                     /locus_tag="ACHK8E_002506"
     CDS             14459..14968
                     /gene="purE"
                     /locus_tag="ACHK8E_002506"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP0344758.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            14965..16032
                     /gene="purK"
                     /locus_tag="ACHK8E_002507"
     CDS             14965..16032
                     /gene="purK"
                     /locus_tag="ACHK8E_002507"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP0344759.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(16365..17258)
                     /gene="ybcF"
                     /locus_tag="ACHK8E_002508"
     CDS             complement(16365..17258)
                     /gene="ybcF"
                     /locus_tag="ACHK8E_002508"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0344760.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(17255..18041)
                     /gene="ylbF"
                     /locus_tag="ACHK8E_002509"
                     /pseudo
     CDS             complement(17255..18041)
                     /gene="ylbF"
                     /locus_tag="ACHK8E_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            complement(18052..19311)
                     /gene="ylbE"
                     /locus_tag="ACHK8E_002510"
     CDS             complement(18052..19311)
                     /gene="ylbE"
                     /locus_tag="ACHK8E_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0344761.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(19321..20988)
                     /gene="fdrA"
                     /locus_tag="ACHK8E_002511"
     CDS             complement(19321..20988)
                     /gene="fdrA"
                     /locus_tag="ACHK8E_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0344762.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
     gene            21305..22354
                     /gene="allD"
                     /locus_tag="ACHK8E_002512"
     CDS             21305..22354
                     /gene="allD"
                     /locus_tag="ACHK8E_002512"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP0344763.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            22376..23611
                     /gene="allC"
                     /locus_tag="ACHK8E_002513"
     CDS             22376..23611
                     /gene="allC"
                     /locus_tag="ACHK8E_002513"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP0344764.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            23622..24407
                     /gene="allE"
                     /locus_tag="ACHK8E_002514"
     CDS             23622..24407
                     /gene="allE"
                     /locus_tag="ACHK8E_002514"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP0344765.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(24536..25681)
                     /gene="glxK"
                     /locus_tag="ACHK8E_002515"
     CDS             complement(24536..25681)
                     /gene="glxK"
                     /locus_tag="ACHK8E_002515"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP0344766.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(25703..27004)
                     /gene="ybbY"
                     /locus_tag="ACHK8E_002516"
     CDS             complement(25703..27004)
                     /gene="ybbY"
                     /locus_tag="ACHK8E_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP0344767.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(27061..28422)
                     /gene="allB"
                     /locus_tag="ACHK8E_002517"
     CDS             complement(27061..28422)
                     /gene="allB"
                     /locus_tag="ACHK8E_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP0344768.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(28483..29937)
                     /gene="ybbW"
                     /locus_tag="ACHK8E_002518"
     CDS             complement(28483..29937)
                     /gene="ybbW"
                     /locus_tag="ACHK8E_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP0344769.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(30107..30985)
                     /gene="glxR"
                     /locus_tag="ACHK8E_002519"
     CDS             complement(30107..30985)
                     /gene="glxR"
                     /locus_tag="ACHK8E_002519"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0344770.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(31085..31861)
                     /gene="hyi"
                     /locus_tag="ACHK8E_002520"
     CDS             complement(31085..31861)
                     /gene="hyi"
                     /locus_tag="ACHK8E_002520"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP0344771.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(31874..33655)
                     /gene="gcl"
                     /locus_tag="ACHK8E_002521"
     CDS             complement(31874..33655)
                     /gene="gcl"
                     /locus_tag="ACHK8E_002521"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP0344772.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(33745..34560)
                     /gene="allR"
                     /locus_tag="ACHK8E_002522"
     CDS             complement(33745..34560)
                     /gene="allR"
                     /locus_tag="ACHK8E_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP0344773.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(34638..35120)
                     /gene="allA"
                     /locus_tag="ACHK8E_002523"
     CDS             complement(34638..35120)
                     /gene="allA"
                     /locus_tag="ACHK8E_002523"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP0344774.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            35350..36276
                     /gene="allS"
                     /locus_tag="ACHK8E_002524"
     CDS             35350..36276
                     /gene="allS"
                     /locus_tag="ACHK8E_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP0344775.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            36345..37439
                     /gene="mnmH"
                     /locus_tag="ACHK8E_002525"
     CDS             36345..37439
                     /gene="mnmH"
                     /locus_tag="ACHK8E_002525"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP0344776.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            37555..37920
                     /gene="ylbG"
                     /locus_tag="ACHK8E_002526"
                     /pseudo
     CDS             37555..37920
                     /gene="ylbG"
                     /locus_tag="ACHK8E_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         9764 a        10148 c         9510 g         9143 t
ORIGIN      
        1 tcttacgcca taatcttgaa agcacaaaag aatacataaa aaataaagac attaacaaaa
       61 agcataaaac gaggctcata taaatataag agcctccata ttttagtcgt ttagaaacaa
      121 attattttaa tgtggtgtgc ttcgtgacaa taaattaata accaacacac cggcacaaat
      181 caacatcatg cctataatgg ctggcaggtc cagccgttgg ccgaaaaatc cccatgacag
      241 taagctaatc aggacaatac cgactcctga ccagatagca taagcaatcc ctgtaggaat
      301 ataagccagc gtctgagcta ataaccagaa tgatgcacaa taacaaataa ttgtaccaac
      361 agatggccat aaccgtgtaa aaccttctga aaacttcatt aaggttgtac caatgacctc
      421 tgcaagtatt gcaccaccaa gataaatata agggttcata gcatattctt tcctgttcaa
      481 actggagaga attgtactac agtttgaact caactcacct gtttcatcat tgtgttccca
      541 ttgatgttct tttatatacc ctcagtaccc gtttcatcgc ggcactctgg cgacactcct
      601 taaaaatcag attcgtgctc acctttcctt cccgttcttc tctggtagcg aaccggtaat
      661 acaccgttcg ccagacctta ccatcaacga ccaggattcc tgcccgcgcc attttagccg
      721 cagcctgatt tatgctggtt acggttgcgc ctgttaccgc ggcaacgtcc tgtgcacaga
      781 agctcttatg cgtccccagg taatgaataa ttgcctcttt gcccgtcata cacttgctcc
      841 tttcagtccg aacttagctt tgatttctgc gatcttcgcc agagcctgtg cacgatttag
      901 aggtctaccg cccatgacag gaagttgttt tactggttca ggtatcgcct caccacggtt
      961 aattcgcgcg gtcatacagg acagttcatc ggcagccttg cgccgtaatt ccgcgtcagt
     1021 caacgcattg gcccgcatgt tctgatacag gttggtaacc agccagtagt gcgcgtttga
     1081 tttccacgga taagactctg catccggata cagaccacgc ttccggcaat actcgtaaac
     1141 catatcaacc agctcgctga cgtttggcag cccggcggta acggatgctt cttcccggca
     1201 ccatgcaaca aactgcccgg gtgatggcag aaatggtcta ttctgccgac gggcaacacg
     1261 cattccggcg ctcacctgtt ccatcgtggt gatcccgttt tcccggaaag ccagaaccca
     1321 ctggcggcgg atttcgttca gttcgttctg gtcacgatta gccaggctcg ccgggaaagt
     1381 tgccagtaac tggctgaata caccgttgat tatctgcgct acctgctgta cctgcggctt
     1441 ttcgtcgtac tgttccggca tgttgttggc gatccgacgc atctgctcac ggtcaaagtt
     1501 aatcatctgt gcggcgatgt ttttcataga tccaccccgt aaatccagtc tgtgtttgtc
     1561 aggtcgagtt ttggtttgct agctgtcacg cctgcctgtt gcttgttacg gttgatttcg
     1621 agttgggtcc acttgtcgcg gagtttggcc ggacttagca cgttaccgga ccagaagttg
     1681 tcctggcatg cccagcggaa cagcacgcac atgtcgcggt ggttacgtcc gtcacgttca
     1741 cgcatcaggc ggatatcgtt agcccaccct gcaaaattcg gttttctggc tgatggcgcg
     1801 atggtcttca ccatgtcaaa catccactct gcggcggtca ggtcttctgc tgtcccccac
     1861 ttgctgccgc tctgaattgc agcatccggt ttcaccacag gaagatcgtt ttctggttgg
     1921 tcagaggatt cgccagaatt ctcggacgaa aaaggtttta tattgtcttt tgttagtttg
     1981 tcttttgtgt ttacctgatt cgggtaaacg cctttacctg atttgggtaa acttttttta
     2041 cctgattcag gtaaatttac ctctttcagg taaactttat ttttcttacc tgattcgggt
     2101 aatgttgacc attcactgac cacattatta atgccggtat tccgcccgct ctgaataaaa
     2161 atcccacgct ttaccagaac actttttgca gcagaacact tgtgcggcaa tatcccggtt
     2221 aattcggaaa gttgctcgtt gctaacccaa tccagttttt tattaaagcc atatgttttg
     2281 cgcatgacag ccagaaagac cagaagctgg tgctgtgtta atccggccag catcacagct
     2341 tccagcaact catttgcaat gcgcgtataa ccatcatcga gatctgccac gcgcggctcc
     2401 ttttgtgccg catccggcac tggaaaattg aatatctcag cagtgtttgc cataattcct
     2461 cccgcaatga gtgtgttacg atttgcacct gaaagtcggt tctgttccag cagaccggct
     2521 ttcgccattt ctgaacctgt catatcgccc ccagcatggt agtaaccatc gccatcaatg
     2581 gaccagccag atctgggtcc acacgaaaca tcgacacaat accttcacta atttccttca
     2641 gtttctggtg gcgtggtgcg ttgagaatga cagcctgttt tgcctcactg agttcctttt
     2701 ccatttcagc caacctagcc atgaagctat cctgctcaac caggtaaccg cgatattcca
     2761 gcggtagtac cgccagaatt gccggggtca gttcacgcac gttatttcgg tatttttcag
     2821 aatcgaattt gttatcgagg aagcggaaca gcttctggcg tgcacggctg acatcatcag
     2881 ggaaatcgat ggtgccgccg ccctgctccc gatactcatt cacaatgagt gtggcaacga
     2941 catcctgatt atctacagcc gaccaggcgc ggacggcatc acggattttt tcgtggcctg
     3001 gcacctgttt tgtttgagaa cgatttatca ccgcagtcgg gctaaatccg ctagtctgtt
     3061 ggtatgtaag tggttgcata attgactcct ttagtttgaa ttgactgtta agttgattgc
     3121 ttattgttaa agagcgtgaa atggaaattt aagctgcgtt cttttcggtg tgtggaaaca
     3181 acttcggaag atccgggcga atctggtatg ccttcactac tccaccagta gccgtaacaa
     3241 tgctgccgac atgttcaggg gatacctttg ctttgttgtg aagccactta tagacggcct
     3301 gctgtgaaac ttcgcaagca gcgcccagtt tcttttgtga accaacgata ttgatcgctg
     3361 ttttgatagc tgggttcata acaacctccg tggttaattt gaatcaagat taaaactatg
     3421 gttgttttta gtcaacaacc attttcgttt gatggaataa aaccttggtt gtacatttgg
     3481 actatgaaaa caacactctc agaaagactt aaagaagcca gattagcgcg aggccttaca
     3541 caaaaggcgc ttggggattt ggtcggggtt agccaggctg ctattcagaa aatcgaaaca
     3601 gggaaagcta atcaaacaac taaaatcgtg gagatcgcga acgctttggg tgtgcgcgca
     3661 gaatggttat cttctggcgt tggaaatatg tcagacagta cagtgcaacc aatacaatca
     3721 actgtcagcc attccaaata cttcaagatt gacgttcttg atatagaagt cagtgctggg
     3781 ccgggagtca tcaaccgtga gtttgtagaa gttctacgct cggttgagta ctcgtttgac
     3841 gatgctcgtc acatgttcga tggtaggaag gcggaaaata tccgcatcat taacgtgcgt
     3901 ggtgacagca tgtcaggaac gatcgaacca ggtgatctgc tgttcgttga tatcacagtt
     3961 aaatctttcg acggtgatgg tatctatgcg tttctgtacg acgacacagc ccatgtaaag
     4021 cgcctgcaaa tgatgaagga taagctgctg gtcatctctg ataacaaaag ctactcaccg
     4081 tgggacccga tcgagaaaga cgagatgaac cgggtgttca tcttcggtaa ggttattggg
     4141 agcatgccgc agacatatag gaagcatggg tagtaccaat taaaaattat caactgggca
     4201 ttgtgctcat tcagtaaaga actaattcct atctttgctc taggtagtaa tattaagcca
     4261 ccgcaataat atctttacct aacggcgtaa gaatcccggt caccgtgccg ggttttcttt
     4321 tgccctcccc tcatcacaca caccgttaaa aaaaccacca taacctcgct tcagttatcg
     4381 ctatgcgatt caagtcacaa aataaatcca tcctaaatac aaccagttat atctaaaaca
     4441 accaataaaa caacttttgt tgttgacgat aaaacaacta tagttttaaa taagttcatc
     4501 gcaacaacac aacgatacgg caactacctg attcaccgtt gcgatgaccg cttagatccg
     4561 cagtttgaat ttcagcaggc ttcggggagt gcgaggggtg aaacggacgc gtgaacgtcg
     4621 gtgtgaccag ctgaaattaa ctcaacattt cataccttag tcgcttcaac gaggcggctt
     4681 agttatgaca accggcggcc atccaccgcc tgaatacgcg cagaagtctc tatatgttca
     4741 gcagcccagc ttacgggcag gagtttttat ggttcatcaa cattatggaa cgcagaccgt
     4801 taatcgaggt gcggtcatgc caggaatgct ggtcaaacac aaagatggta cctggactgc
     4861 atcagctaat ttacgcggac ggctttatct gcatcgcggc atcgagcgca cttatacccg
     4921 tgatttgctc gtggaagttt ttctcgacgg acgcggtaac ggcctgaatc actaatcccc
     4981 tttcctgttt tcctaatcag cctggcattt cgcgggcgat attttcacag ccattttcag
     5041 gaggtcagcc atgaacgctt attacattca ggatcgtctt gaggctcaga gctgggcgcg
     5101 tcactaccag cagatcgccc gtgaagagaa agaggcagaa ctcgcagacg acatggaaaa
     5161 aggcctgccc cagcacctgt ttgaatcgct atgcatcgat catttgcaac gccacggggc
     5221 cagcaaaaaa gccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc
     5281 agaacacatc cagtacatgg ttgaaaccat tgctcaccac caggttgata ttgattcaga
     5341 ggtataaaac ggatgagtac agcactcgca acgctggcag ggaagctggc tgaacgtgtc
     5401 ggcatggatt ctgtcgaccc acaggaactg atcaccactc ttcgccagac ggcatttaaa
     5461 ggtgatgcca gcgatgcgca gttcatcgca ttgctgatcg tcgccaacca gtacggcctt
     5521 aatccgtgga cgaaagaaat ttacgccttc cctgataagc agaacggcat tgttccggtg
     5581 gtgggcgttg atggctggtc ccgcatcatc aatgaaaacc agcagtttga tggcatggac
     5641 tttgagcagg acaatgaatc ctgtacatgc cggatttacc gcaaggaccg taatcatccg
     5701 atctgcgtta ccgaatggat ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc
     5761 agagaaatca cggggccgtg gcagtcgcat cccaaacgga tgttacggca taaagccatg
     5821 attcagtgtg cccgtctggc cttcggattt gctggtatct atgacaagga tgaagccgag
     5881 cgcattgtcg aaaatactgc atacactgca gaacgtcagc cagaacgcga catcactccg
     5941 gttaacgatg aaaccatgca ggagattaac actctgctga tcgccctgga taaaacatgg
     6001 gatgacgact tattgccgct ctgttcccag atatttcgcc gcgacattca cgcatcgtca
     6061 gaactgacac aggccgaagc agtgaaagct cttggattcc tgaaacagaa agccactgag
     6121 cagaaggtgg cagcatgata ccggacatta tcctgcagcg taccgggatc gacgtgagag
     6181 ctgtcgaaca gggggatgat gcatggcaca aattacggct cggcgtcatc accgcttcag
     6241 aagttcacaa cgtgatagca aagccccgct caggaaagaa gtggcctgac atgaaaatgt
     6301 cctacttcca caccctgctg gctgaggttt gcaccggtgt ggctccggaa gttaatgcta
     6361 aggcgctggc ctggggaaaa cagtacgaga acgacgccag aaccctgttt gaattcactt
     6421 ccggcgtgaa tattactgaa tccccgatca tctatcgcga cgaaagtatg cgcaccgcct
     6481 gctcccccga tggtttatgc agtgacggca acggccttga actgaaatgc ccgtttacct
     6541 cccgggattt catgaaattc cggctcggtg gtttcgaggc aataaaatcg gcttacatgg
     6601 cccaggtgca gtacagcatg tgggtgacgc gaaaagatgc ctggtacttt gccaactatg
     6661 acccgcgcat gaagcgtgaa ggcctgcatt atgtcgtgat tgagcggaat gaaaagtaca
     6721 tggcgagttt tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg
     6781 aaattggttt tgtatttggg gagcaatggc gatgacgcat cctcacgata atatccgggt
     6841 aggcgcgatc actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc
     6901 tgttatcaga aatccactga aagcacagcg gctggctgag aagataaata ataaacggga
     6961 ggcggtatgc acaaagcatc tcctgttgag ttaagaacga gtattgaggt ggcacatagc
     7021 cttgctcaaa ttggagtcag gtttgtgcca ataccagtag aaacagacga agaatttcat
     7081 acgttagcca catccctttc acaaaagctg gaaatgatgg tggcgaaagc agaagcagat
     7141 gagagagacc aggtatgaca accactgaat gcatttttct ggcagcgggc ttcatattct
     7201 gtgtgcttat gcttgccgac atggggcttg ttcaatgaca cctcagcaag aaaacgccct
     7261 tcgcagcatt gcccgtcagg ctaattctga aatcaaaaaa gccagacagc attttccgga
     7321 taaaaacgtc gatgacattt gccgtagcgt actaaagaag caccgcgaaa cggtaacgct
     7381 gatgggattc acaccgactc atttaagcct ggcgatcggc atgttgaacg gcgtctttaa
     7441 ggaacggtaa acatgaaaag caaaatcatc agggagctac aggctccttt tttattattc
     7501 gcatttaccc tcaagcgtat taaccaacaa ttcagggatt aatgaaagat ggcagacatc
     7561 attgattcag catcagaaat cgaagaatta cagcgcaata cagcaataaa aatgcgtcgt
     7621 ctgaactacc agactgtatc cgcaactcat tgttgtgagt gtggcgatcc gatagatgag
     7681 cgaagacgcc tggctgttca gggttgtcgg acttgtgcga gttgccagga ggagatcgaa
     7741 cttaagaaca aacaataggg actgtgatgg cctcaaagca gcaaatttca acatcgtcca
     7801 actgaggtgt aaaaatgttc agaatcattt ttcctaacac ctggtacgtc gaccaccacg
     7861 gcactccctg caaaatcctg cgttctaccc acaacaaagt tcactacatc cgaaaaggca
     7921 gaacatgtat cgccagcatg ttccgcttta atcatgactt tgaacctgtg aataaagctg
     7981 atgcagatcg gatagcagaa gagatcgaaa cggcagaaca cattaagaag ttacgtgaca
     8041 tgcgttcaaa aagcagaggt aaccatggaa tcatacagcc tcacactcga tgaggcctgt
     8101 cagtttctta agatatccag accaaccgcc accaactgga tacgaacagg ccgcctacag
     8161 gcaacacgta aagatccaac caagccaaaa tctccttacc tcacaacacg gcaagcctgc
     8221 attgcggcgc ttcagtctcc gctgcatact gtccaggtga gcgcgggtga tggcataaca
     8281 gaggaaagaa aatgtcactc ttccgcagaa atgaaatatg gtatgcctcg tattcgctcc
     8341 cgggcgagaa acgaattaag gaatctcttg gcacaaagga caagcggcaa gctcaggagt
     8401 tgcacgacaa gcgaaaagca gaactctggc gagtagaaaa gctaggggat ttacctgatg
     8461 tcacttttga agaggcctgc ctaagatggc ttgaggaaaa agctgataaa aaatctctcg
     8521 attcagataa aagccggatt gagttctggc ttgaacattt tgagggtata aggcttaaag
     8581 atatctcgga ggcaaagatt tactctgctg taagcagaat gcataacaga aagacgaaag
     8641 aaatatggaa acagaaagtt caggccgcca tcaggaaagg taaagaaccg cctgtttatg
     8701 aaccaaagcc agtatcaact cagacaaagg caaagcatct tgccatgata aaggccattc
     8761 tccgtgctgc agaacgcgac tggaagtggc tggaaaaagc gcctgtcatc aagataccag
     8821 cggtcagaaa caagcgagtc agatggctgg aaaaggagga agcaaaacgc cttattgatg
     8881 agtgccccga accactgaaa tctgtcgtca agtttgcgct ggcaactggt ctgagaaagt
     8941 cgaacatcat aaatctggaa tggcaacaaa tcgacatgca gcgacgagtt gcctgggtga
     9001 atccagaaga gagcaaatca aaccgcgcca ttggtgtggc gctgaacgat accgcctgta
     9061 aagtgttgcg tgatcaaata ggcaagcatc acaaatgggt gtttgtacat accaaggcgg
     9121 ctaagcgagc agatggaaca tcaacgcctg cggtcaggaa gatgcgcatc gacagcaaga
     9181 catcatggct atcagcttgt cgtcgtgcag gaattgaaga tttccgtttc catgacctca
     9241 gacacacctg ggcaagctgg ctgattcagt caggcgtccc attatcagtg cttcaggaaa
     9301 tgggcggatg ggagtccata gaaatggttc gtaggtatgc tcaccttgcg cctaatcatt
     9361 tgacagagca tgcgaggaaa atagacgaca tttttggtga taatgtccca aatatgtccc
     9421 actctggaat tatggaggat ataaagaagg cgtaactgat tgaattgtaa tggcgcgccc
     9481 tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc aactgagcta
     9541 agggcgcatt gataccacgg tgtgctgtaa tcgcgtgaat tatacggtca acccttgctg
     9601 agtcaatggc ttttgaacca gttgctgaac aagtgtacga ccgcatcaga tgtagctcac
     9661 tatggcggca aaccaacgac ggttatcatg ataggtgtga atcagccagg tacagaaggt
     9721 tttacttttc agaaaacgaa aactgacagc gcgccacgct tctgacaaaa tagacgcatc
     9781 cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga ttattgacgg
     9841 taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg cgcgtattgc
     9901 agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca
     9961 aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct cccgctctta
    10021 tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgatg cgctgaatgc
    10081 cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta ttgataacgt
    10141 caaagtgctg gaacgtatac acccggacaa agacgtggac ggtttccatc cttataacgt
    10201 cggtcgcctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg gtattgtcac
    10261 gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga ttggcgcatc
    10321 gaatatcgtt ggccgcccga tgagcatgga actgctactg gcaggttgca ccaccaccgt
    10381 gactcaccgt ttcactaaaa atctgcgtca ccacgtagaa aatgccgatc tactgatcgt
    10441 tgccgtcggc aagccgggct ttattcctgg tgactggatc aaagaaggcg caattgtgat
    10501 tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga
    10561 cgcggctaaa cgcgcctcat acattacgcc tgttccgggc ggcgttggcc cgatgacggt
    10621 tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc cacagggtga
    10681 gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc gacttgctga
    10741 aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg
    10801 tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc gggcagacag
    10861 tgagttttgc aggtcacagc gtacaggttg ttgcctgatc cctttcccgg cctgtaacaa
    10921 caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat gctgatagca
    10981 tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg ccgttcccct
    11041 ttgcattggt ttagggttgg gttcgaaagt catccctccg cgtctgttat ttgccggaat
    11101 cgtcctggcg atgctgcccg atgccgacgt gttgtcgttt aaatttggcg ttgcttacgg
    11161 caatgttttt ggtcatcgcg ggtttaccca ttcgctggtg tttgcgtttg ttgtcccgct
    11221 gttatgtgtg ctgattgggc gtcgatggtt cagggctggg ctgattcgct gctggctgtt
    11281 tttaaccgtc tcgttgctat cgcacagctt gctggattcg gtaaccactg gcggtaaagg
    11341 ggttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc aggtgattaa
    11401 agtcgcgccg tttgcattgt ctcgttacac cacgccgtac gggcataagg tgattatttc
    11461 tgaattgatg tgggtgtggt tgccggggat gctactgatg ggaatgttgt ggtggcgcag
    11521 acgataaccg gatgcgaaaa ctcgcatccg gcaatagcgc aattacttac gacgccaggt
    11581 ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt cacgcgccgc
    11641 atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa ttaacgcttc
    11701 aatctcagcc acttcactgt cgtctgcctg cgcgccgctt tgcaggaacg cttccggttc
    11761 ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagacgcca ttgcattcgc
    11821 cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa acagtacgga
    11881 ataggcttcc ggggtgttga aatcgtcgtt catcgcctca ataaagcgcg cttcaaacgc
    11941 ttcgccaccg gcaggagcaa cggttttgtc tgtgccgcgc aaggcagtat agagacgttc
    12001 cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcaactgac tgcgatagtg
    12061 acccgacatc aggaagtaac gcacagtttc cgcatcgtag tatttcagca catcacgcac
    12121 ggtgaagaag ttacccagcg atttagacat cttctcgcgg tcaaccatca ccatccccga
    12181 gtgcatccag tagttcacat actgaccatc atgggcacag gtggactggg caatttcgtt
    12241 ctcgtggtgc gggaacatca ggtcagaacc gccgccgtgg atatcaaagt ggttacccaa
    12301 ctgtttgcag ttcattgccg aacactcaat gtgccagcca ggacggcccg cgccccacgg
    12361 agacggccag ctcggttcgc cctctttcga catcttccac agaacgaaat ccatcgggtt
    12421 gcgtttgtcg tcgaccacgt caacgcgcgc gcctgcctgc agctgatcga gatcctgacg
    12481 cgacagcacg ccataagttg gatcggtcgg gacgtcgaac atcacgtcgc cgttgtccgc
    12541 cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt cggcgatatg
    12601 gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa aatctttgtg
    12661 catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt cattggcgcg
    12721 tttgatgatt ttgtcgtcga tatcagtaat gttgcgcaca tacttcagct tatagccgag
    12781 gaaacgcaga tagcgcgcaa ccacgtcaaa ggcaacaaag gtacgcccgt gaccgatatg
    12841 acagagatcg taaacggtga ttccacacac gtacatgccg acttccccgg cgtgaatagg
    12901 cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg aagattccgt
    12961 ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg cgaatcagca
    13021 tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat atatgacccg
    13081 aaccgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact ttccacacca
    13141 atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca gttaaaaact
    13201 tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt gtaatcaacg
    13261 gctttatgat tcagggcggc ggttttgaac cgggcatgaa gcaaaaaacc accaaagacc
    13321 cgatcaaaaa cgaagctaac aacggtctga aaaatacccg tggtacactg gcaatggccc
    13381 gtactcaggc accgcactct gcaactgcac agttcttcat caacgtggtt gataacgact
    13441 tcctgaactt ctctggcgaa agcctgcaag gttggggtta ctgcgtgttt gctgaagtgg
    13501 ttgaaggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt agcggtatgc
    13561 accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc gagtaatcgt
    13621 ggcgacactc tttattgcag atcttcatct ctgcgcggaa gaaccggcga tcaccgccgg
    13681 ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata ttcttggcga
    13741 tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc agatggcggc
    13801 agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg gcaaccgtga
    13861 ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acgttattgc cggaagaaaa
    13921 ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc tgtgcaccga
    13981 tgacgccggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc aaacgctatt
    14041 cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgcg cgaacagcaa
    14101 agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg cggtggtcag
    14161 tgcgatggaa aaacatcagg tgcaatggct gattcacggg catacccatc gcccggcagt
    14221 acatgaactt atcgccaatc agcaacctgc tttccgcgtc gtgctgggtg cctggcatac
    14281 tgaaggttcg atggtgaaag tcacggcgga tgacgttgag ttgatccatt ttccatttta
    14341 aaaaacgcgc aactttgctg atttcataac cgcgcaaccg ttttccttgc tctctttccg
    14401 tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt taagacgcat
    14461 gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa gcgactgggc
    14521 taccatgcag ttcgccgccg aaatcttcga aatcctgaat gtcccacacc acgttgaagt
    14581 ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagtg ccgaagagaa
    14641 cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgccag gcatgattgc
    14701 cgccaaaacg ctggtgccag tgctgggcgt gccagtacag agcgccgcac tgagcggtgt
    14761 cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta cgctggcgat
    14821 tggtaaagct ggcgcggcaa acgcggcgtt actggcagca caaattcttg cgactcatga
    14881 taaagaactg caccagcgtc tgaatgactg gcgcaaagcc cagaccgacg aagtgctgga
    14941 aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa cgggcagtta
    15001 ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc ggtcgggctg
    15061 gacgctgaac cggcggcggt gcctgttcaa caaagtgtga ttaccgctga gatcgaacgc
    15121 tggccggaaa ccgcattaac ccgcgagctg gcgcgccatc cggcctttgt gaaccgcgat
    15181 gtgttcccga ttattgccga ccgtctgact cagaagcagc ttttcgataa gctccacctg
    15241 ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt gtttgatcgt
    15301 ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg cggtcaatgg
    15361 cgtttacgcg caaatgaaac cgaacagtta ccggcagagt gttacggcga atgtattgtc
    15421 gagcagggca ttaacttctc tggtgaagtg tcgctggttg gcgcgcgcgg ttttgatggc
    15481 agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt gcgcaccagc
    15541 gtcgctttcc cgcaggccaa tgcgcagcag caagcgcaag ccgaagagat gctgtcggcg
    15601 attatgcagg agctgggcta tgtaggcgtg atggcaatgg agtgttttgt caccccgcaa
    15661 ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg gacacaaaac
    15721 ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct gccgttaccg
    15781 caaccggtag tgaatagtcc gtcggtgatg atcaacctga ttggtagcga tgtgaattat
    15841 gactggctga aactgccgct ggtgcatctg cactggtacg acaaagaagt ccgtccgggg
    15901 cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac cgcgacgctg
    15961 gaagccttaa tcccgctgct gccgccagag tatgccagcg gcgtgatgtg ggcgcagagc
    16021 aagttcagtt aattgatgct cattttcttg ccggatgcgg cgtgaacgcc ttatccggcc
    16081 caccgctcca acaccgactg taggcctgat aagacgcgcc agcatcgcat caggcatggt
    16141 gtgccgattg caggatgcgg cgtgaacgcc ttatccggcc taccgttcca acaccgactg
    16201 taggcctgat aagatgcgcc agcatcgcat caggcatggt gtgccgattg ccggatgcgg
    16261 cgtgaacgcc ttatccggcc taccgttccg acaccgcctg tagacctgat aagacgcgcc
    16321 agcatcgcat caggctagat gtgccaattg cctggtgccc atcactacag cgaaatacaa
    16381 gtccccgctt cgcccgccag cgtctcttca atccgcgata acgccccaat ccacgcgggt
    16441 ttgccacggc ttctgacata gccactcacc gcagtcacct tcggccccat cgaaccatcg
    16501 gctttggcaa atggcgctaa ctcatccggt gtggcatggc gaatggcacg ttgctgcggc
    16561 gttccccagt tttcatatac cgcatcagca tcggtgagga tcaccagtcc atcggcatta
    16621 atctgctcgg cgagcaacgc agcggcgaga tctttatcaa ttaccgcttc actccctgcc
    16681 ccgtctttcg tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg
    16741 agcaacaact cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc
    16801 cggcgtaaat atttaccatc acgtttcatc tgccagccat aagccgcttc cagtgcctct
    16861 tgttcttctg gctgataaac cggaccgata aacttctctg gctgcaaaaa cgccggatca
    16921 tcaggcgaaa cttcaatgcg cgtcagcact gtcgtgacag gcggcatctg cggctgtgcg
    16981 ctcaagctct gcgccagcat atagccaatc atcccctggc tttccgccac cagcacatcc
    17041 agcggatacg gttctacctc tttccacgcc agattctgta acgccagcag ccccacctgc
    17101 ggcccgttgc cgtgaacaat cgccagccga taagaacggg ccaggcgcgc cagtgcgggt
    17161 acagcactgg caatgttgcg atattgattt tctgccgtca gcgcctcacc gcgctggagt
    17221 aaggcgttgc ccccaagagc cacaaccagt gttttcatga ttttccttgt aataattgtt
    17281 ggccaagcca gaaccccagc agcgtatctg cccctgacgt atgccccagc gccagcagcg
    17341 aatcaatcgc aacagcggta cgtttcgggc aactcagagc atgaataaag tgcaggagtg
    17401 gcgaggcgaa gtatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa
    17461 cgagttgaag attgtcggaa caggcaaaaa acgggcgacc ggagcgcgca tctaaagcgc
    17521 cataatacca ggccgccagc agcattccgc tcagcgtgtc atcatgactt ggcgttaatc
    17581 ctgggcctta cgccattgag cgcggcctga aaacagtgac gaaactggcg taattcagca
    17641 ggaagcggat cgcctgccgc caacgccagt ggcccgaaaa gcccggtttc ctccgcgcgt
    17701 tgcatccatg ccgctgcaag cggttgagga tgcgcaggcg gcgtaatacg cagcaaacaa
    17761 taacgctgtg gctgtttaac cgtgaaacgt ccgaggcgaa tcccttgagc cataacctgt
    17821 ggtcgttcat tgccgcataa tccaccgcat aacgcatcga attgcgcgcg gcgaagcacc
    17881 catcctccgg ggccgaaacc actaccctga cgatgcagcg ttaacagttc gccgctttcc
    17941 gtcatcaggt taatcgcccg ccgccacact cccgctaacc gccaggactg gcgataattc
    18001 ggtgcgctac tactggcaag cagaggatgg atgatcgtca ttgtgcgttc ctcaaccaat
    18061 ccccatgctt tcagccagtg cttccagcgc ctgctcaaag cacgccagcg gtgcccgcac
    18121 ggtgcctgcg ccaatctgcc cgatccccgc ctctttatgg gcgattccgg tattgatgag
    18181 cggcgtaatg ccggtttcta ccacgcgacg aatatccagc cccaggcacg cgccctggaa
    18241 atcccagccg gggatctgca actgcatatt gcgttcaagg aagatttccg ccatctcttc
    18301 agataccgct ctggccgctt ccatgccacc cgcaccgaca aagcgcgtta cgccaggcgc
    18361 tgcgatcatc gccgcaccgc caataccaaa ggtttcggta attgcgctat cgcccatatc
    18421 cgggttcgcc tgctcctgcg agaagccggt gaaaaacagc ccttggggag tgtttacagg
    18481 cgcagtaaac cagcgttcgc ccagcccgct tacccgaatc ccgaacatat tgccgttgcg
    18541 ggtcatagcc gtgacgatgc tgcctgcgcg gatcatcgca ccagcatcca tcgccgcctt
    18601 gcagtaagcc atcgcgaggt taaggaagaa ctgatcggtc acgctgagga aatccatcac
    18661 ttccgcgatg tgctgtttat catgatcgag gcgagcaatg tgtggggcca gcgcacgcat
    18721 taacagtgct gaggaagcaa tattgcgttg atggaactca tcgcccatgg taatgccctg
    18781 cgccatcatc gcggtgagat cgataccgcg ctccatgcgc cccagcgccg cgcttaatac
    18841 tggcattaac acatcgcgca tccagcgatg gcgagtcaga acatcttcgc cgtaagcgcc
    18901 aaaacgcatc actttgccga taccttcgtt gaggttgcag tacgcccggt tgccgtcggt
    18961 cacgttctcg accaccagca tcggcatact ggcagaagta atgccgccca ttggcccgac
    19021 ggcattcaca tggtgacaag gaatgaagtt cacttccccc tgatccagca ttgccagcgc
    19081 ctgcgcttca tctttcgccc agccttcaaa cagacatgcg cccacacatg cccctttcat
    19141 gggtccggtc atctcctgcc agcgcattgg cggcccggcg tgaagcagtg ttttgccctc
    19201 gtttagttcg ctgataagtg aagaagccgg ttgcacatcc agccagtgtg gacgagcgcg
    19261 acgaatttgt tcgataaccg cagcattggc ttgcgccact gatgtaaaca tgggaacccc
    19321 ttattgcaaa cgttctaata aacgaaccag ttttttattg ccaccggcga ctggcgacca
    19381 ttggtaatgc acaaccggtt tgctggcgct ttgtagctcc agcgcaaagc tgcgtaatcc
    19441 gatgttaatc acggcgacgt tttccagtaa tgacggtgta tgtggctgtg tggcaggcga
    19501 gagcggatga attaacgccg ccgccagcaa ggtggcttcc ggcagcgaac tcacgaccgc
    19561 aatccccgca tcttccagcg tggcgatttg ctgcgagcgg cattgcgggt cacgttcagt
    19621 gcctgtcacc gtggcaatgg cgtacagcgg ttggctgtca gaacgcgcgg cacaggcttt
    19681 ttgccaggcg ctcaccagcg aggcggcagg atcggcggtc gcaccgaagc caatcacgac
    19741 atcaagcagc aacacgcgca cttgcggttt agcgccgaga tcggcaatta acaggttgcg
    19801 taaggtcgga tcgatcatcg gatggggacg cccgacggtg taaaaatcat cgcccaggtc
    19861 gaggatctga tgtccgtcgg cgtccagcat catgccatgt tgatgggtat cgtcggcttc
    19921 cacgccaagg tgtccggcaa gtaatcccgc cgcttcagca gccagcgtac cgccggtata
    19981 caagccgcaa ataaatccgc tgctgacagg cgctattgcg ttacgtcgcg cagtgacgcg
    20041 tgaaagcaga caagccaggc gtgcggcctc atccagcgag gaggcaaacc agacattctc
    20101 gtcgcgggcc accgccgggg tataacctaa aaacagcgcc accgtcggtt tgccagttgc
    20161 tttcatggca ttaacaattt tcagacgcac cgcttcggca ggtggttttg aaacaaatgc
    20221 cagcacttcg cttttctcgt ctgcactgag catttccagc gccgtcagcg cactgatgcc
    20281 gcccacttca cggctgaggt cgcgcccgcc aaggccaatc gcgtgagtaa ttccctcccc
    20341 tgccagcgca atctgtgagc acagctcctg aatcccggta ccggaagcgc caatgacgcc
    20401 aatgttgcct tccggtatca cgttagcaaa agccagcggc gtgccggcaa tcatcgacgt
    20461 accgcagtcc ggccccatca ccagcaagcc tttttcccgc gcgcgggttt taagttggat
    20521 ttcatcttcc agcgtgacgt tatcggagaa catcatcacg ttgaggttgc gatccagcgc
    20581 ctggttcgcc agctccgccg catactcacc agccactgaa atcagcgcca gattggcatc
    20641 gggtaatttc tgacaggcac tgtcccagcg acgcacctgc gtcaacgcct ggctgctggc
    20701 tgacccctgc gccagttgtt ttagcgcctc ttcaagctgc tgcataatcg cctgcgcgat
    20761 ccccgcatcc gccgcttcgc tacgaattgc cacgcaaata tcgttcggcg tggcgttatt
    20821 aaaatcgtca tgccagaaac ctgtggtatc taataacgct ttattggcgg gcgtacccat
    20881 cattacggaa acatcatcaa cattttctga ttcgctgagt tttcgtgaaa taatcattaa
    20941 actgaccgaa tcctgaaaac accctttttt aataaaggcg tggatcataa caactcctta
    21001 aaaattgcac ccatggcaat tgcacgagca gtgttaatgt ggcgattacg gtatcgatga
    21061 caacgagtaa ataaggtgat gaatatcaca cagggattat taacaaaaga attcaaataa
    21121 ccgccatgct attacgtgtt attttttaat aagcgtggtt gttttaaaaa ccaggttaaa
    21181 agcgagcgga ttatcaaata aattctaact atattttttt gcctgtctgg atcacataat
    21241 ccagatattt tccctgtatg ttaattgcag tcatgcttca caccgtcgtt aaaaaggaag
    21301 acagatgaaa atcagtcggg aaacactcca ccagctaatt gagaataaac tctgccaggc
    21361 tgggttaaaa cgtgagcacg ctgcaaccgt ggctgaagta ttggtttacg ccgatgccag
    21421 agggatccac tctcatggcg cggtgcgcgt ggaatactac gcggaacgca tttcaaaagg
    21481 cggcaccaac cgcgaaccgg agtttcgtct tgaggaaacc gggccgtgct cggcaatttt
    21541 acatgccgac aatgccgccg gacaggtcgc ggcgaaaatg ggtatggaac atgccatcaa
    21601 aaccgcccag caaaatggcg ttgcggtggt cggtatcagc cggatgggtc acagcggcgc
    21661 aatctcttat tttgtgcagc aggcagctcg cgccgggtta attggcattt cgatgtgcca
    21721 gtccgatcca atggtggtgc cgtttggcgg cgcggaaatt tactacggta ctaacccact
    21781 ggcctttgcc gcgccgggag aaggcgacga gatccttacc tttgatatgg cgactaccgt
    21841 acaggcatgg ggaaaagtgc tcgacgcccg ctcgcgtaat atgtctatcc cggatacctg
    21901 ggcggtcgat aaaaacggag caccaacaac cgatccattt gcagttaacg ccctgctccc
    21961 tgccgccgga ccgaaagggt atggcctgat gatgatgatt gacgtcctct caggcgtctt
    22021 actcggctta ccgttcgggc gacaggttag ttcgatgtat gacgatttac acgccgggcg
    22081 taatttgggg caattacata tcgttattaa tccgaacttt ttctcctcca gcgaattatt
    22141 ccgtcaacat cttagccaga ccatgcgcga attaaatgcc attacccccg cgcccggttt
    22201 taatcaggtt tattatcccg gacaggatca ggatattaaa caacgcaaag ccgccgtcga
    22261 aggcatcgaa attgttgatg atatttacca gtatttaatt tccgacgcgc tttataacac
    22321 gtcatacgaa acgaaaaatc cctttgcgca ataattattg agacaggact ttcttatgat
    22381 tacacatttc cgtcaagcca tagaagaaac gctgccctgg ctttcctctt ttggcgctga
    22441 cccagcgggt gggatgaccc gtttacttta ttcgccggaa tggctggaaa ctcagcagca
    22501 gtttaaaaaa agaatggcag caagcgggct ggaaacacgt ttcgatgaag tggggaattt
    22561 atacggtcgc ctgagtggca ccgaatatcc acaggaagtg gttctgagcg gttcgcatat
    22621 cgataccgtg gttaacggcg gtaacctcga cgggcaattc ggtgcgctgg cggcgtggct
    22681 ggcaattgac tggctgaaaa cgcaatacgg cgcgccgtta cgtacggtcg aagtggtggc
    22741 gatggcagaa gaagaaggca gccgcttccc gtatgtcttc tggggcagta aaaatatctt
    22801 tgggctggcg aatcctgacg acgtgcggaa tatctgtgat gccaaaggaa atagttttgt
    22861 cgatgcgatg aaggcttgcg gatttactct gccgaacgcc ccactaactc cgcgtcagga
    22921 tattaaagcc tttgtcgaac tgcatatcga acagggctgt gtgctggaaa gtaatgggca
    22981 gtcaattggc gtggtgaatg caattgtcgg gcagcgtcgt tatacggtga cgctgaacgg
    23041 cgaatcaaac catgcaggca ccacgccgat gggttatcgt cgtgatacgg tttacgcttt
    23101 cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag atgggcgacc cgctggtgct
    23161 tacctttggc aaggtggagc cgcgtccgaa tacggtgaat gtggtaccgg gtaaaaccac
    23221 gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg cgcgatttca cccagcagtt
    23281 agaaaacgac atgcgggcga tttgcgatga aatggacatt ggtattgata tcgatttatg
    23341 gatggacgaa gaacccgtgc cgatgaataa ggagctggtc gccaccctga cagaattgtg
    23401 tgaaagtgaa aaacagaact accgggtaat gcacagtggt gccgggcacg acgcgcaaat
    23461 tttcgcgcct cgcgtgccga cctgcatgat tttcattccc agcatcaacg ggatcagcca
    23521 taacccggcg gaacgcacca atattaccga ccttgccgaa ggggtcaaaa cgttggcact
    23581 catgctttat caacttgcct ggcagaaata aggagttata aatgggatat ttaaataacg
    23641 tcaccggtta ccgcgatgat ttactggcta accgtgcgat tgtgaaacac ggtaatttcg
    23701 cactgttaac accagacggt ctggtaaaaa atattattcc gggctttgaa aattgtgacg
    23761 cgacaatcct ctccacgcca aagctgggtg cctcttttgt tgattatctg gtcacactgc
    23821 atcaaaacgg cggcaaccaa cagggcttcg gtggcgaagg cattgaaacg ttcctgtatg
    23881 tgatctctgg aaatatcact gccaaagccg aagggaaaac atttgcctta agcgaaggtg
    23941 gctatcttta ttgcccgcca ggctccttaa tgacgtttgt taacgcccag gccgaagaca
    24001 gccaaatctt tttatataag cgtcgctata ttccggtaga aggtcatgcg ccgtggctgg
    24061 tttctggcaa tgccagcgaa ctggaacaca ttcattatga aggcatggac gatgttattc
    24121 tgctggattt tctgcccaaa gagttaggtt ttgatatgaa catgcatatc ctctcttttg
    24181 cgccaggtgc cagccacggt tatatcgaaa ctcacgtcca ggaacacggt gcctatattc
    24241 tttccggtca gggggtttat aacctcgaca ataactggat cccggtgaaa aaaggcgatt
    24301 acatctttat gggcgcttac tccttacagg ccggttatgg cgtagggcgt ggtgaagcgt
    24361 ttagttatat ttactcaaaa gattgcaacc gcgacgtaga gatttaagtc ataccactcg
    24421 cctgattatt tttagctatg ttggattttg ccacggttta ttccgtggct ttttttgtag
    24481 tcaattcagg taaaaaaaga tgcgatataa atcgcatccg ctttaaaggt aagagtcagt
    24541 ttttaattcc ctgacctatt ttaatggcgc aggcaatatt tcgcgcgctg ttaaagagat
    24601 tggtttcacc gctggcgagc acttcggcta gaggtgccag acgtggcaaa atgctgaata
    24661 ccgcgtcaat gccgtactgg tgcaccactt ccacgccatc acccaatacg ccagcaatcc
    24721 caatcaccgg gacattaaac tgcttcgcca ccgacgccac gcccagcggc gctttaccac
    24781 ctgccgtttg cgagtcgatg cgcccttccc cggtgatcac cagtgctgcg ccctgcactg
    24841 cctgcgcaag attgaccgca ttcaacacaa tctcaatacc cggtttaata tccgcattga
    24901 ggaataccgc cgccgcaatg cccataccgc ccgccgcgcc accacccgcc atctggcaga
    24961 cgttgatttc agtttgctgt tgtaaaacac gggcgtaatt ttgcagcccc tgctccagct
    25021 cttcgaccat ttccggcgtt gccccttttt gcgggccgaa caccgccgcc gcgccgcgtg
    25081 cccctaccag cggattatca acatcacatg cgacttcaat atggcagttc gccagacgcg
    25141 gatcgcattc atccatctca atacttgcca cgcgtgctaa attgccgccg ttagccgcca
    25201 gcacctgacc gtaggcatca aggaaacgca caccgagcgc ctgcgccatg cccataccgc
    25261 cgtcaaccgt cgcgctacca ccaatgccga gaataatatg gcgaatgccg ttatccagcg
    25321 catggcgaat taactccccc gtaccaaaac tggaggccag caacggatta cgcttttcag
    25381 gcgcgaccag catcaggcca cttgctgctg ccatctcaat caccgccgtt ttcccgtcgc
    25441 cggtaatgcc ataaaaagca ttcacttttt cgcccatcgg cccgcagact tcaagcttca
    25501 cgatgttacc gtccgtcgcg gcgaccatcg cctccaccgt cccttcgccg ccatccgcta
    25561 tcggcaaaca gatatagtgc gcatcgggaa agagggtcga aaacccggct ttaattgcct
    25621 gacaacattt ttctgcactt aagctctctt taaaagagtc tggcgcaatg acaatcttca
    25681 ttatcaatcc tttttcaaca aattattcga tgcgttccca cggaataaga ttatccataa
    25741 gaacagccag taaaattccg accagtaagc cgttactgag cagaggacga agcgttaatg
    25801 gaaggtcttg cagatacaca ggcggtaatg ccattaaaaa tatgccgaca aataacggca
    25861 atgcgagtcg ataaatattg cgagcggtaa acgtgatttg ctggctaaac accagcgcgg
    25921 aaaagagtaa aggcagataa gaaaccagca tgaccgcgct actcactggt aaagggatac
    25981 tgcaaaacag tcgcgtgagt gccggaacca gtgccaccag caggcaaata acgctgccat
    26041 aaataaacga acgccgcgtg taatcgctag tctgggttaa taaaccaatg gatgaaacaa
    26101 acggtgaaaa tggaattact gccagtggca cggttatcag tgtcataaat ccggtcgcca
    26161 caaagctgcg acgataacgc gtattccccg cgccctgctg cggataaaaa acatccgtgc
    26221 cccgaatcgc accgtaggta ttgctgatat ttaccagacc tgtaatcacc gccgtcagaa
    26281 taatccccgg cgacaaagca ccaccactgc cgagcgggaa ccactgccag tgcaactcac
    26341 cggagagcga atgcgaagaa ggaaagcaaa agtaccacaa caaccagccg gttatcgtac
    26401 cgaccagcag gccgtaacgg gcaaaacgtt gtggcaggaa aataatcatc gccagtacca
    26461 ggcacatcac cgcgaccgag agcgcgaacg gcggtaattg aattttaaaa ttcgggtcgg
    26521 ctatgccaaa cggcagcccg agcatacctt tgaaaaagat agtggtcagt tgcgcaccca
    26581 gcatcaacat aaacaagacc atcaccgacg gcgtaaataa ccgtgccagg cgatgtccta
    26641 atccgctaaa accaatcaac atcgtcagca cgccggagag cgcaattccc accgccagac
    26701 tggtagcgat atcgttgatc ggtgtcccgc gcgatgcttc gccaagggtg atagtcagga
    26761 tggttcccca ccacaggccg cccggcccct ccataatagc gcgacgatga ccgcagaacg
    26821 cctgagcgaa gcaggccagt gcggtagcaa gaaaagcata ttgcgtgagc gtaagcaggc
    26881 tactttgcgg caactgaaaa gcagaaagta gcgtaggagg aaccacaacc gtgttgcaaa
    26941 aaataaagaa aaaccactga aatcctgata acaggctttc gcggctgact gcaaaattga
    27001 acataaaaac accttaaccg gagaatgccc gccgcaagga cgggcattgc aggggccaga
    27061 ttactgctga tgtttaagga taaattgacc tttcggcgca acagggaagc cctgttcgat
    27121 gtcgtaaatc acatcaccac gtaagatggt tttcgtgata cgcgcgccaa tggtacggcc
    27181 aacatacggg ctgactttgt ggcgatattc cagatcgtca ttggtaagaa catagctgct
    27241 attcggctga atgaagacga agtcggcatc ttttcctggg gcgatacggc ctttttgctg
    27301 cagaccgaaa atatctgctg cgttagtcgc cattaatttg ccgaacatcg gcagagacat
    27361 accgcgtttc tgtaccgctt catcgaacat cacgtccatg cagctttgca gaccggcgat
    27421 accaccccat gctttcatga tattgccggc tttcatttcc ggcggacatg gagagtggtc
    27481 ggaaaccagg cagtcgattt caccgttaaa cagtttttcc cacatgcctt tctggttttc
    27541 cagatcgcgg atcggcggtg aacacttcgc cagagtaccg atttcttcga actgatcggt
    27601 atcaagtaca aagtaatgcg ggcaggattc acaagtaacg tcctgacctt cctgacgtgc
    27661 acgagtcact tcttcaacgc cttccgggct gctgatatgg caaacgtgca gacggcaacc
    27721 ggcaactttc gccagataca gcacgcggcg aatcgcttcc acttcggtaa ataccggacg
    27781 cgaagccaca tagtcatgag cagttacgcg accttcacgc ttcgcttctt cgcccagttc
    27841 gtcacagatc agcgcgtttt cgcagtgcac cagcaccggc tgccccagtt cgcccagctt
    27901 ctgcgcacct ttgaagaact gccagtcgtt tacatcacgg aagtcgttgt cgataccgcg
    27961 atcgccacag gtcgcaacga agcatttgaa gccgacaacg cccacttcat ccagctcatg
    28021 cagacggtcg atgttgtaag acaccaggcc accgagttgt gccgcatcga tagtcagctt
    28081 gcctttagcg gcatcgaact tcagttcaat tgaagcgcgg tcaaccgttg ctggcagctg
    28141 gttgagcggc atttcgatca tggtggtgat accacctttt gccgctgcgc gagtaccagt
    28201 ttcataacct tcccagtggc tacgacccgg ctcagaaata tgggtgtgcg catcaaccat
    28261 gcctggagaa accaccagac cagacgcatc catcacttct tttgcatcgc ccagatcctg
    28321 accgatagca gcaatttttc cgcctttaac ggcgatatct acaacgcgag cttcgttttc
    28381 taaaataacg gtgccgtttt taatgattaa atcaaaagac ataacaaact ccttgtaaaa
    28441 tacagattgg ttttttaact caatgttcaa tatcgggatt aattaacctg tgactttttg
    28501 ttctcctgtt ttttctgctg ctgtacgttt ttttaataac gcgtaggcag cgaatgcgac
    28561 aataacgccg acaaaccatg aaacgcgtga taaaggctcc atgaacggaa taaatttacc
    28621 accgagagat aaaataacgg cgaccaaagt tactgagaac gcggtaaggt taaagccgtt
    28681 atcgtaatat ttgaaatcac ctgctgcggt atacagttca tcaagattaa tttgtccgcg
    28741 catcaccata aaataatgcg ccatcatgac accaattacc ggacccagca ttccgccgat
    28801 aatatcgagg aacagataaa tactgtcctg attttccatt aatttccacg ggcagatcac
    28861 caggctgata atactggcaa tcagtacgcc gtttttatag gtcagtttcg tcggtgcaat
    28921 agcggcaatc tgataaccgg ccggaataat attaccggtg gcgttagtgg aaatggtcgt
    28981 catcagaata accagtaccg caaagaacga ggcgaacagg ctgtcccaac gctgaacaat
    29041 atccagcacg ttccaggtgt cagcgccgta atgaatactg gctccggcaa taatacatac
    29101 cccggcgacc gcaaacagaa tataggccac aactaaaccc agcgtttgcc ccagcgcctg
    29161 ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg ctcaccgccg gtgccgccca
    29221 gaccgcaacc accgcgttta tcaccaccag aaacaggaag ccactgtttt ctgctttctg
    29281 aataccgccc ggaatgtagt caaagattgg accgatcccg accagcgaaa tcgcccaaat
    29341 cgccataccg ccgaaaacga tatagatgca cgggttaaga atggcagtga atttatttaa
    29401 aacttttccg ccgccaaaac caataccaac gttaaccagc cagaagagta aaaaagtaat
    29461 taatcccggt agagaaaggc ctaacagcgt gaaatcgcca ccgagagtca aaaatcccgg
    29521 ccagattttg ccaatcagaa tcaagcaggc cagtgacccc gcgtaacatt gcaggccaaa
    29581 ccacataatg gcagcaatac cgcctcttaa taatccggga aacagtgcac cacgtacgcc
    29641 gtaagaagca cgcaggatca tggcaaacgg tacgccgtat ttactgcccg cagcaccgtt
    29701 taataccatt accgcggcaa tgaaaaaggc actgaggata attgccagca taatactaaa
    29761 ggtagacaag ccgagaataa aaaagccgcc aaccatcaca taattgggaa cgttatgaac
    29821 cgaacccatc cataaggtaa aatagttaaa tgttttccag gtccgctgac tttgcgtttt
    29881 cggcagtaga tcttcgctat agccgcgttg ctggaatagt tttctctgat gttccataaa
    29941 tccctcttaa tttcacgtat cgaataacgt tgttttaatc tagctatgag gaacaaataa
    30001 aataaaaata ttatttttaa tactgaagca acatctttgg ctgctttctt tttttaatat
    30061 tgctggccga ttcattttct gatatcggcc atttttattg cgggtatcag gccagtttat
    30121 ggttagccat taattccagc gcctgcacta atgcagagtg atccaactgg ctgccaccgt
    30181 ttgccgcaca ggtattaaac aattcctggc aggtcgcggt gtttggcagg ttcagcgcca
    30241 gtgctttcgc actttgcagt gccaggttga gatctttctg gtgcagcgca attttgaaac
    30301 ctggattaaa ggtgcgttta atcatgcgct caccgtgaac ttccagaata cgtgaggaag
    30361 caaagccgcc catcagcgcc tggcgcacac gtaccgggtc cgcaccggct tttgaagcaa
    30421 acaacaaggc ttcagaaacc gcttcaatat tgagcgccac gataatctga tttgccactt
    30481 tgcaggtttg accgtcgccg ttaccgccca cgagagtgat atttttaccg agcagttcaa
    30541 acagcggttt tacacgttca aatactgctt catcaccgcc aaccataatc gataacgtcc
    30601 cttcacgcgc gccgatttca ccgccggaga ctggcgcatc gagataatcg ccgcccagtt
    30661 cattcacctg acgagcgaaa cgtttagttt caatcgggga aatggagctc atatcaacaa
    30721 tggttttgcc cttcagcgag gctttggtac aaccattttc accaaacaga acgtcttcaa
    30781 cctgtggcgt atccggcacc ataataaaaa tgatgtccga tgcatctgtt acctggcgcg
    30841 cagtttcaac actaacggcg cccagtgaca gtaattcatc agcaaccgga ccaatggttg
    30901 tgacatgcag ttgatgaccg gcacgcgcca gattaatggc catcggtgta cccataatgc
    30961 ctaagccaat aaatcccagt ttcataatta acctctttta aatttcgctt ttcctgaatt
    31021 cagacaacac gatcccctgc caacttagcg gcctaaaaaa gcattgtctg aatagcgtta
    31081 cgttttaacg gtacggatcc atccagcgta aaccggcttc cgtggtggtt tggggtttat
    31141 attcacagcc aacccaaccg ttgtagtcag aattttcaat taccgtaaag agatattcgt
    31201 aattaatttc tccggttccc ggttcgccgc gatgcggatt atcagcaatt tgcaggtgac
    31261 caattttatc ggcccactga gtcatggtat tagttaattc accttccatc cgctgcatgt
    31321 gataaatgtc atactgaatt ttcaaattgc agcaaccaac atcgtcaatc agtttcagtg
    31381 cctgccgggt tccggtgaaa tggaaaccag gaatatcaaa atggttaata ggttcaatca
    31441 gtaataaaat atcttctttc atcagcatat tcgcagcata acgcaggttt tctacaagcg
    31501 ttgtgtgaat ctgttcactg ctgaaaccag tcggcgtttt accgaccaga cagttaattt
    31561 ttttattacc cagagcacga gcataacgaa tcgctgctgc tacgccatcc cgaaactctt
    31621 cttcacggcc aggaatacag gcaatacccc gctcgcccgc cgcccagtca cctgccggta
    31681 aattgtgcag cgtatgttcg agtttattac tcgccagcac ctgttttaat tcttcaatgt
    31741 cgtagtcata agggaacata aattcaacgc cgcgaaaacc acactgcgca gctttctcaa
    31801 aacgggcgag aaaatcatat tcaccaaata acatcgataa attagcagag aaacgtaaca
    31861 ttatttatct cccttattca tagtgcatga aacaggcttc agtcggtgca tccgctgcgt
    31921 tatcggcgat atcttcaaat tccatgacgt tatccagttc actgcccatc gaaatattgg
    31981 tcacacgctc gagaataact tccacgacta ccggtacccg atattgcgcc attaagattt
    32041 tcgcctgttc aaaggctggt gcaatatctt ccggtttgaa aacgcgaatt gctttacaac
    32101 ctaaaccttc cgctactttt acgtggtcga cgccataacc gttcacttcg ctggagttaa
    32161 tattttcgaa cgcgagctgt acgcagtaat ccatatcaaa ggcacgttgc gactggcgaa
    32221 tcagacccag ataggcgtta ttgaccagta catggatgta cggaatgttg aactgtgcgc
    32281 caacggctaa ttcttcaatc aggaactgga agtcgaagtc accggaaatc gccaccacat
    32341 tgcgctctgg atcagcggca caaaccccca gcgccgccgg aatcgtccag cccagcgggc
    32401 ctgcctgccc acagttgatc cagtggcggt ctttaaagac atgcagcatt tgtgccgcag
    32461 cgatttgtga caggccaatg gtggtgacat aacaaacatc gcggccaaag gctttgttca
    32521 tctcttcata cacgcgctgc ggtttcaccg gcacgttgtc gaagtgggtt ttgcgcagca
    32581 aagtgcgttt gcgctgctgg cagtcggcga cccattcttt acgacacggc agacgacccg
    32641 ctttttgcat ctcctgcgcc acttcaacca gcagcgtcag cgccgcttta gcatcagaga
    32701 caatgccgag atccggacac agcacgcggc caatttgcgt cggctcaata tcgatatgaa
    32761 cgattttgcg cccttcggtg tatttctcta ccgaaccggt atgacggtta gcaaaacggt
    32821 taccgatacc aaacaccatg tcggacgcca gcagcgttgc gttaccgtaa cgatgcgcgg
    32881 tttgcagacc caccatcccg gccatcagtt catgatcgtc gggaatacag ccccagccca
    32941 tcagcgttgg gatcaccgga atgctggtca gttcagcaaa ctgttgtaac agtgcagctg
    33001 cgtcagcatt gattaccccg cccccggcaa caatcaccgg acgttcggcc tggattaaca
    33061 tttctaccgc tttttcgatc tgcatacggc tggcagcagg tttgtagacc ggcagcggtt
    33121 cgtacatgtc aggatcaaac tcgatttccg caacctgaac gtcgaacggt aaatccacca
    33181 gtaccggacc cggacgacca gaacgcatca ggtgaaatgc ctgttgcagc acgcgaggca
    33241 ccagcgccgc ttcacgtact gtaaccgaca ttttgctgac cggtttagca attgcttcaa
    33301 tatcaacggc ctggaaatct tctttatgca gacgggcgcg cggagcctgg ccggtaatac
    33361 acagaatagg aatggaatca gcagaagcag aatagagcgc ggtgatcatg tcagtgcctg
    33421 ctggtcccga ggttcccaga catacgccga tattccctgc cgttgcgcgg gtataacctt
    33481 ccgccatgtg cgaagcacct tccacatgac gcgccagaat gtgacgaata ccgccgtgct
    33541 tacgcatcgc tgaatagaaa ggattgattg ctgctcccgg aacgccgaag gcggtggtga
    33601 taccttcttt ctccagcaca tacattgccg cgtcaacggc tctcattttt gccattttat
    33661 tcctacctct attaattgga aaaattttcc aactttcaaa ttttgtcaaa aaaacccgct
    33721 ccgcagagcg ggatgcgaca gacactatgg atgtgctttc agtcccaacg ccgtgctgat
    33781 atcgcgggcg gtgtctctga ccagctcacc ctgactgaca aaacgatctt ctgtcagtct
    33841 tgatgaaggc ccggagatgg agatagcggc aacaacacta cctacatcat cgtaaattgc
    33901 tgaagctatg caattcaggc cgacaacatg ctcttcttta tctacggtat agcccagttc
    33961 acgcgcttgt tccaggtcct tcagcaaggt gggcatatcc acaagcgtag ttggcgtaaa
    34021 ctgctgcaaa ccggtttgca gaatgatgct catcaactcc tcttccgcca gcggataaag
    34081 cagcgccttg cccgcaccgg aagcatgcag cggcagacga ctgcccagcg gcgcacacat
    34141 cctgaccatc gatttacact ctaactgacc aattaatacc gcttcattgc cgttacggat
    34201 cgcgacattg accgtttcgc cggaaagtaa catcaggcgg cgcataaacg gcccggcgac
    34261 ggagagtaca tcgcggttat ggatgtacgc tgcaccgacg ttaaagacac ctaatcctat
    34321 atgccaccag cctaactgac tgtcctgata gacaaaatcc gctgcctgta aaaccttcag
    34381 caagcgaaag gtcgtggaga gcggcaaatc cagattgaga gaaatatcgc taaccgacga
    34441 acttccccca cttttttcca gatattgcag aatcgcaatt ccccgctcta acgcctgtgc
    34501 gcccttctgt gccacaggct ccgcctgtcc tggcctgccg cgccgtctaa cttccgtcat
    34561 acctacgtta actccgacct acaagtgagc ataaacaatg aacaattcgt ttatcttatg
    34621 caagtcggtt gcaggcgtca cgcaaaacag agttcctgtt caggaatact ttcaacatca
    34681 cagttgtcac tgccgccgcg atcgatggtc agaaaatcgg tgacgcgctg ccaggcgaaa
    34741 agtgggtgat gccagacgtt acgatggtaa ttcaccccct gttcaccgtt ggtaataaac
    34801 gcccgcagcg ttgacaggtc tggtttgtcg tcacctaacg ccacgactac cacaaacact
    34861 tcacctttca tcgggataaa cgcctgtgta cccaacggat gacgttcgag ttcgtgaata
    34921 gtcagcggca gattcgccgg ttgcgcgcgg ttaatgctga taagcgtacg gtcttgctca
    34981 agaatctcaa ccagcgccaa atcgtggtaa cgctctacca ggccattgtt aatatggaaa
    35041 aaatcccgtt gctgcgtttc gattacgtcg ccataagcac taaaggcttc ctgacttaac
    35101 ggtaatacct gaagtttcat ctttttgtcc ttgtaacata attccgttca accaccgccg
    35161 accggtcagc ttggtttcaa taacaggcaa tctaaaacct taagagaaca acatcaatac
    35221 aaaagttgga atatttttcc aaaactagat ttagtccaca aattgaacag ggttatcatg
    35281 taaaaatact gataaatcag aggataagaa aatcacgcaa cttgcggatt taaaagggga
    35341 tacctgaaga tgttcgatcc agaaaccttg cggactttca ttgcggttgc tgaaacagga
    35401 agtttttcaa aagcggcaga acgattatgt aaaaccacgg cgacgatcag ttatcgcatt
    35461 aaacttctgg aagagaatac cggagtagcg ctgtttttcc gtacgactcg tagcgtgacg
    35521 ttgacggcgg ctggcgagca tctactttgt caggccagag actggctgag ctggctggaa
    35581 agtatgccca gcgagctgca acaggtgaat gatggcgtgg aacgccaggt gaatattgtc
    35641 atcaacaacc tgctctataa cccccaggcc gtcgcccagt tgctggcgtg gctgaatgaa
    35701 cgttacccct ttacccagtt tcacatctcc cgacaaatct atatgggcgt ctgggattcg
    35761 ctattatacg aaggtttttc actggctatc ggcgtcacgg gaaccgaggc gctggcaaat
    35821 acctttagtc ttgatccctt aggatcggtg caatggcgct ttgtcatggc ggcggatcat
    35881 ccgctggcga acgttgaaga gccgctaaca gaagcacagt tgcggcgctt tccggcggtt
    35941 aatattgaag acagcgcccg cacactaacc aaacgcgtcg cctggcgatt gccggggcaa
    36001 aaagagatta ttgttcccga tatggaaacg aaaatcgccg cccatctggc gggcgttggc
    36061 attggttttt tgccaaaatc actttgccag tcaatgatcg ataatcaaca actggtcagc
    36121 cgggtaatcc caacgatgcg ccctccttca ccattgagtc tggcatggcg caaatttggc
    36181 agcggcaaag cgatagaaga tattgtgacc ttgtttaccc agcgcaggcc ggaaatcagc
    36241 ggatttttag aaattttcgg caacccacgc agttaaaaat caagtttcct tgcgcgttgg
    36301 cgcgtaaaat agcgcgcaag ctgatttgag gttcactttt tactatgcaa gagagacaca
    36361 cggaacagga ctatcgtgcc ctgctgattg ctgatacgcc cattattgat gttcgcgccc
    36421 ccatcgagtt tgagcaaggc gcaatgcctg ccgctatcaa cctgccgtta atgaataacg
    36481 atgaacgcgc cgccgttggc acctgctata aacagcaagg gtcagacgcg gcgctggcgc
    36541 tgggtcataa actggtggcg ggtgaaattc gtcagcagcg tatggacgcc tggcgggcag
    36601 cgtgcctgca aaatccgcaa ggtattctct gctgcgcccg tggcggtcag cgctcacata
    36661 ttgtgcaaag ctggttgcat gcagcgggga ttgattatcc gctggtgaaa ggcggttata
    36721 aggcactgcg ccagaccgcg attcaggcga ctattgaact ggcacaaaaa ccgatagtgc
    36781 tgattggcgg ttgtaccggc agcggtaaaa cgctgttagt gcagcaacag ccgaacggtg
    36841 ttgatctgga agggttggcg cgtcatcgcg gttcggcgtt tggtcgcacc ttacaaccac
    36901 aacttagcca ggcgagtttt gaaaacctgc tggctgccga aatgctaaaa accgacgccc
    36961 gtcagaattt gcgcctgtgg gtgctggaag atgaaagccg gatgatcggt tcgaatcacc
    37021 tgccggaatg cctgcgcgag cgaatgaccc aggcggcgat tgcggtagta gaagatccgt
    37081 ttgagatccg tcttgagcgg ctgaacgaag aatatttcct gcgtatgcat catgatttta
    37141 cccacgcgta cggcgacgaa cagggctggc aggagtattg cgaatacctg catcacggac
    37201 tttcggcgat taagcgtcgg ctggggctac agcgctataa cgaactggct gcaaggctgg
    37261 atgcggcgct gacaacgcaa cttaccaccg gcagcaccga cggtcatctg gcctggctgg
    37321 tgccgttact tgaagaatat tacgacccga tgtatcgcta tcagcttgag aaaaaagcgg
    37381 aaaaagttgt ctttcgcggt gagtgggcgg aagtggcgga atgggttaag acgcgataat
    37441 gagggacgta tttgtattag tcgcgacttg atctgacaca tggccttgaa aggttgagag
    37501 ttaccggttt tgatatgagt gtcgaatcct tatccaaaac aagaggtaac tctcatgctt
    37561 catactgcca atcccgtcat caaacacaaa gccggtttgc tcaatctggc tgaagaactc
    37621 agcaacgtat caaaagcctg taaaatcatg ggcgtttcgc gtgatacgtt ttatcgttac
    37681 cgcgaactgg ccgctgaagg cggtgtggat gcgcagatta atcgtagtcg ccgcgctcct
    37741 aaccttaaga accgtaccga tgaggcaatt gaacaggctg ttgttgatta cgccgtcgct
    37801 ttcccgacac atggtcagca ccgggccagc aacgagctgc gtaaacaggg ggtttttatc
    37861 tccgacagtg gtgtccgttc tgtctggtta ctccataacc ttgagaatct caaaagacga
    37921 tactgaacaa attttatcag gtgacgttcc gtaaaaagtt gtatggagtt ctcgatgcag
    37981 tcggcgcgac tatcagtacc cccgctgaac cctggactat cgggccgccg tactggggca
    38041 tgtcaccctg gcgtgccgct ggttttccgc taatggttta tttcctcttt ctttcccggc
    38101 gagaatttat tttaatccgg aaggattatt tattttcaga tagggactca cattaaacac
    38161 gtaacatttt aattaatgca tggctgaata tgtattacac aatgtcagat atcgtaaaag
    38221 accagtgcaa ttctatgtaa actctctggc tgttcatttt atttgttgtt tcagggtcgg
    38281 aactcaccct caataaacaa ctgaatacga gtagatattc atcgtctgag ctatatggct
    38341 ttacacaata gccgacatgt cttttctcag tcagggatta tacttttcct cactacgaag
    38401 ttgacgactt cgatatggga tagactctta attcaagcaa ttactctgca caagcatcag
    38461 gtaacccagc tccaatattt gttataaaat aatgcgtaac attaatttca ccaacaaaaa
    38521 aaaggtttac gctatcccct tgccctggta ttttaccata tttac
//
LOCUS       DAZGTJ010000040        38092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000040 DAZGTJ010000000
VERSION     DAZGTJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.040"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            189..1133
                     /locus_tag="ACHK8E_002527"
     CDS             189..1133
                     /locus_tag="ACHK8E_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344777.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESASLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1214..1636
                     /locus_tag="ACHK8E_002528"
     CDS             1214..1636
                     /locus_tag="ACHK8E_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344778.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
     gene            2184..2633
                     /gene="glmS"
                     /locus_tag="ACHK8E_002529"
     CDS             2184..2633
                     /gene="glmS"
                     /locus_tag="ACHK8E_002529"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFP0344779.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            2633..4021
                     /gene="glmL"
                     /locus_tag="ACHK8E_002530"
     CDS             2633..4021
                     /gene="glmL"
                     /locus_tag="ACHK8E_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFP0344780.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4040..4525
                     /locus_tag="ACHK8E_002531"
                     /pseudo
     CDS             4040..4525
                     /locus_tag="ACHK8E_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
     gene            4915..6312
                     /locus_tag="ACHK8E_002532"
     CDS             4915..6312
                     /locus_tag="ACHK8E_002532"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFP0344781.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
     gene            complement(6457..9090)
                     /gene="mngB"
                     /locus_tag="ACHK8E_002533"
     CDS             complement(6457..9090)
                     /gene="mngB"
                     /locus_tag="ACHK8E_002533"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP0344782.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(9108..11024)
                     /gene="mngA"
                     /locus_tag="ACHK8E_002534"
     CDS             complement(9108..11024)
                     /gene="mngA"
                     /locus_tag="ACHK8E_002534"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP0344783.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            11194..11916
                     /gene="mngR"
                     /locus_tag="ACHK8E_002535"
     CDS             11194..11916
                     /gene="mngR"
                     /locus_tag="ACHK8E_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP0344784.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(12020..12889)
                     /gene="sucD"
                     /locus_tag="ACHK8E_002536"
     CDS             complement(12020..12889)
                     /gene="sucD"
                     /locus_tag="ACHK8E_002536"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP0344785.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12889..14055)
                     /gene="sucC"
                     /locus_tag="ACHK8E_002537"
     CDS             complement(12889..14055)
                     /gene="sucC"
                     /locus_tag="ACHK8E_002537"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP0344786.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(14149..15366)
                     /gene="odhB"
                     /locus_tag="ACHK8E_002538"
     CDS             complement(14149..15366)
                     /gene="odhB"
                     /locus_tag="ACHK8E_002538"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP0344787.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(15381..18182)
                     /gene="sucA"
                     /locus_tag="ACHK8E_002539"
     CDS             complement(15381..18182)
                     /gene="sucA"
                     /locus_tag="ACHK8E_002539"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP0344788.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(18483..19199)
                     /gene="sdhB"
                     /locus_tag="ACHK8E_002540"
     CDS             complement(18483..19199)
                     /gene="sdhB"
                     /locus_tag="ACHK8E_002540"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP0344789.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(19215..20981)
                     /gene="sdhA"
                     /locus_tag="ACHK8E_002541"
     CDS             complement(19215..20981)
                     /gene="sdhA"
                     /locus_tag="ACHK8E_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP0344790.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(20981..21328)
                     /gene="sdhD"
                     /locus_tag="ACHK8E_002542"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(20981..21328)
                     /gene="sdhD"
                     /locus_tag="ACHK8E_002542"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP0344791.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(21322..21726)
                     /gene="sdhC"
                     /locus_tag="ACHK8E_002543"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(21322..21726)
                     /gene="sdhC"
                     /locus_tag="ACHK8E_002543"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0344792.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            22107..22214
                     /locus_tag="ACHK8E_002544"
     CDS             22107..22214
                     /locus_tag="ACHK8E_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344793.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            22420..23703
                     /gene="gltA"
                     /locus_tag="ACHK8E_002545"
     CDS             22420..23703
                     /gene="gltA"
                     /locus_tag="ACHK8E_002545"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP0344794.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            24093..24659
                     /locus_tag="ACHK8E_002546"
     CDS             24093..24659
                     /locus_tag="ACHK8E_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0344795.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            24710..27169
                     /gene="ybgQ"
                     /locus_tag="ACHK8E_002547"
     CDS             24710..27169
                     /gene="ybgQ"
                     /locus_tag="ACHK8E_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0344796.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            27184..27915
                     /gene="ybgP"
                     /locus_tag="ACHK8E_002548"
     CDS             27184..27915
                     /gene="ybgP"
                     /locus_tag="ACHK8E_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0344797.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            27912..28973
                     /gene="ybgO"
                     /locus_tag="ACHK8E_002549"
     CDS             27912..28973
                     /gene="ybgO"
                     /locus_tag="ACHK8E_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0344798.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            29126..30172
                     /gene="abrB"
                     /locus_tag="ACHK8E_002550"
     CDS             29126..30172
                     /gene="abrB"
                     /locus_tag="ACHK8E_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0344799.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(30169..30960)
                     /gene="nei"
                     /locus_tag="ACHK8E_002551"
     CDS             complement(30169..30960)
                     /gene="nei"
                     /locus_tag="ACHK8E_002551"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP0344800.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(30996..31730)
                     /gene="pxpA"
                     /locus_tag="ACHK8E_002552"
     CDS             complement(30996..31730)
                     /gene="pxpA"
                     /locus_tag="ACHK8E_002552"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP0344801.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(31720..32652)
                     /gene="pxpC"
                     /locus_tag="ACHK8E_002553"
     CDS             complement(31720..32652)
                     /gene="pxpC"
                     /locus_tag="ACHK8E_002553"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP0344802.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(32646..33302)
                     /gene="pxpB"
                     /locus_tag="ACHK8E_002554"
     CDS             complement(32646..33302)
                     /gene="pxpB"
                     /locus_tag="ACHK8E_002554"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP0344803.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(33325..34068)
                     /gene="ybgI"
                     /locus_tag="ACHK8E_002555"
     CDS             complement(33325..34068)
                     /gene="ybgI"
                     /locus_tag="ACHK8E_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP0344804.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            34339..35820
                     /gene="dtpD"
                     /locus_tag="ACHK8E_002556"
     CDS             34339..35820
                     /gene="dtpD"
                     /locus_tag="ACHK8E_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP0344805.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(35863..37281)
                     /gene="phrB"
                     /locus_tag="ACHK8E_002557"
     CDS             complement(35863..37281)
                     /gene="phrB"
                     /locus_tag="ACHK8E_002557"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP0344806.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(37278..37787)
                     /gene="ybgA"
                     /locus_tag="ACHK8E_002558"
     CDS             complement(37278..37787)
                     /gene="ybgA"
                     /locus_tag="ACHK8E_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP0344807.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(37929..38092)
                     /locus_tag="ACHK8E_002559"
                     /pseudo
     CDS             complement(37929..38092)
                     /locus_tag="ACHK8E_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         9381 a        10063 c         9231 g         9417 t
ORIGIN      
        1 tattgtttca ttagtaataa tcacttaaat gtatcgatga aacaggtatg acttagcggg
       61 agtgattgtt tctgagtgaa cagagaccac tttcgcctga taaatgaaaa atgagcgatt
      121 tttgcaagtt agtttttcta tagccgagta cgctcagcgc ctcgcttgat tacaatgagg
      181 ttgctgctat gagtcctgat attatcagtt ctgctgtcaa tcgcttctca ggttggccat
      241 ctaatgatcc tctaaaaaca gataaagaat caatgacatc acccatgact gtgcaggtaa
      301 taaagatgat tgccgccatc cttgacccac atcggatagt cgttggaatc gatacacttt
      361 atacaacgca gcgcgaaaca tttacctcag agagtcaaca gctcattgaa tcagcattgc
      421 aaacacttca tgtgatgaat ttaaataacc tggaaacaaa tctcgatgag gaaggtcact
      481 atctggcttt tggatataac gttgacactc ctgacagaat aaccgtttct gcttataatg
      541 aaaaaaagcc tggctgctat ggccaatgtc ttttaataca ggatgacgaa acagacttat
      601 ttaacctgat aaaagttaaa atttttgaag ccaggcataa ttatgtcgct caattgatga
      661 gcgatccgga atttatgttc aaatttagtt atagcgctca acaaaataga ttagaacctc
      721 atcttctggt tcccacttat ttccctctaa aaacaaacga accggttacg caagaggatg
      781 tactgttact ttatcgtttc tttaaaatgg atggtgattt taatatatta acatcagatg
      841 aatacatgag tatcatgaca cctctgatga agtgtgagcg cagcatccac gaccacaaca
      901 ggtatgtgac gggcaaagat acccttcttc tggactatcc tccttctggt aaccagatat
      961 atttccatat ttttcctgac gaatcagcat cactagtcct ttatttttca aacaatgaca
     1021 ttgaatgttt cgtctttgaa agagatattc cgagccagta tcgctttttc aaaatgttta
     1081 ccaatctggc actggttatt gatcagttaa ccgatgccaa tcaaatactt taatttttat
     1141 atatcccctg tggcaaatat tactaaacaa tgcccgggga ttaattttta ttactcacgg
     1201 aaagatttaa ataatgatcc catctcaaat ctccttcaac acccttccag tcaatgcaac
     1261 atacgcttca gagccaacag ttgacacaca aaaattctct gacatcctct actcagcggg
     1321 gtgttcccta aaggatctgg cttatctgag cagatgtctg gcatcaatac atccgactat
     1381 cgccaaaaac atttatgaaa cagaaaatct cagcgatcaa aaattactac atattgattg
     1441 ccgtagtaca aatgaaatca agataaatat cttttttggt caacaacgag aaggattaat
     1501 tgaaattaat tctgacacag tcattttttc gatactgtct tcattgattg actccaccat
     1561 ttctaaattt caacaccaaa accccgttaa cggttctgta aatcctgacc tattgtatga
     1621 ggattatact gattaaatcc ctcctgtcgc gaaaaatggc ctgcgttatt gccagacgta
     1681 ataaaacttt tgattaacgt ttctcgtact ggtagcacct tcatgtactt ttctttttta
     1741 cgaaaaatca aaagagctaa caaaggtcat aattcgcatg aatcattaat tatttgttat
     1801 tttcaacatt atttttacat taagcagaga tgaattgcag tgaaattcga cttaactttt
     1861 atgaaatgca tttactcatg gctaacccca tatcactaaa atgcaaatta gaagttgatc
     1921 acaattatct caatacatca aatcaagtta attttaaaaa tttaacattc atgacattag
     1981 ttgacataac acactgaatt aacatgagaa tttgaaaaca cactttaaac aatcatttcc
     2041 cgaaagacat tttgaaaata gatcaattgc aacattacgg tcagataaag acactcctgt
     2101 gatattaaac ctgtgccgtt atgagcggcc ataaatacga cgtgtgtaac ttattttact
     2161 ctctgtctta caaaggatta cccatgaaga aagcaacact tgtgattggc gttattggcg
     2221 cggactgcca tgcagtaggc aataaagttc tggaccgcgt ttttagcaac catgacttca
     2281 gagttattaa tctgggcgta atggtcagcc aggacgaata tattgatgct gccattgaaa
     2341 ctggtgctga cgcgattgtc gtctcctcta tctatgggca tggcgatatc gactgtctgg
     2401 gcatgcgcga acgctgcatc gagcgtggac tgggggacat cctgctctat gtgggcggta
     2461 acctggtggt cggaaaacat gacttcgctg acgtggaaac caaattcaaa gagatgggat
     2521 ttgatcgcgt ctttgcccca agtcatgact tagaagatgt ttgtcagctg atggcccacg
     2581 atatcaacca acgtcatgac gttgatactc gtattcttga agaggcaatc tgatgcaaat
     2641 cgtctctgtc gatattggat cgacgtggac caaagcagcc ctcttcgccc gggaggggga
     2701 cgcgttaacg ttggttaacc acgtactgac cccaaccacc acccatcatc tggcgaaggg
     2761 gtttttctcc agcctggacc aggtgttaaa cgtagatgat gccctccccc tgttaaacag
     2821 tggtgaggtt gcattgaagt attcctcctc tgccaaaggt gggctggcgg ttgccgcaat
     2881 gggattggtg ccttctatca ccctggaaac cgcgaaagta accgctcact ctgcgggtgc
     2941 taaaattgcg caatcttacg cctacaagct gaaccgccgt gatatccagg cactggaaga
     3001 aacgcagccc gatattctct tgttcaccgg cggcaccgat ggcggagaag aaagctacgg
     3061 actgaataac gcccgtgtgt tggcagaatc caaactcgac tgcgccatta tttatgccgg
     3121 aaaccgggat attcaggacg aggtgcagga gatcctcggg cataaagacc tgaccatcgt
     3181 cgacaacgtt ctgcccgatc ttgaccatcc gaacccttta gctgcacgcc aggctatctg
     3241 cgacatattc cttaaacgca tcgttaaagg gaaagggctg gatgtcgttg ttgataaaac
     3301 aggcgaagaa ccaatgccga caccgtggac agtctttgaa ctggttaagg caatcagtaa
     3361 tgtcgatcac agctggaaag agttcatgct gatcgacatg ggtggcgcaa ccaccgatgt
     3421 ctattccgcc agtgccaaca cgctctcgcc cgacactgtt ttacatggcg taccggaacc
     3481 cttcgtaaaa cggacggtgg aaggagatct gggaatgcgc gtctcggcaa tggtggttgg
     3541 tgaaagtgcg aaagagttag tcaatgtgaa ttttgctcag caacccgcac agctggacgc
     3601 tttttatcat tacctccgtc atctagtcgc tcatcccgac tacttacccg cgaatgcaga
     3661 agaaaaatat ttcgataccg tcctggccgg actgtgtgtg ggttatgcca ccgaaagaca
     3721 tgcgggcacc aaaaaagagg tctgcacctg cgtcggcaat gtcgatttac agatgggacg
     3781 tgacctgaca accgtgcgta aagtcgtcgg atccggcgga tggctctcgc gtgccagtca
     3841 gttcgatatg catcactggt tgaaataccg cgagttaaac gacgacggta aacgcattct
     3901 cttaccgacc gaatttgaat attaccgcga ttcccggggg ctattaccac tgctggcaaa
     3961 cgtcgccaga gtcgatccgc tggccgccgc gcgcaccagt attcaatgct taaccctata
     4021 acaattaagg ctgcaatcaa tggaacttcg aaataagaaa ttaacccatg acgaattcat
     4081 gaccgagcgg caacaggtac tgaaaacctg ggaaaccggc aaggatgttg aaaactttga
     4141 agatggcgtt aaataccaac aaaccattcc tgagcataaa cgcttctctc ttgcgctgtt
     4201 gaaagcagac aaggaaggta aaacccttag ccaaccgcgc gcaggtgtgg cgctgatgga
     4261 cgaacacatc gaactactga aaacactaca agaagagtgc gatctgctgc cgagcaccat
     4321 cgatgcatat acccgtctga atcggtacga agaagcggct gtagggatta aaaaatccat
     4381 cgaagccgga acctctaaac tgaacggttt accggtcgtt aaccacggcg ttgcagcttg
     4441 ccgtcgcctc accgaaactc tgcaaaaacc gctacagatc cgccacggca cgccagatgc
     4501 gcgtctgctg gctgaaatct cgatgtgtat acatgaatat actctaaata agtcaagttg
     4561 caggaaggga acaaataatg aatttccaga agcttacata agtaagtgac ctgggtgagc
     4621 gaacatcgat gcagcacatg caacttgaat tatgacaagt atacaatgat aaatttagat
     4681 accctatgtt atatattatc tatgtccggt taaattgaat gagtcatggc cttaaaagac
     4741 ttcacaaaag ataattcctc tttaacaata agccagattt accacttgta acacttgcta
     4801 tcgccacata cactacgcgc gttttctttc ccttttgctg gctgctgacc ggaaagacaa
     4861 aattaccctc gccatacagg gggttgttta acgtctatat ttcaggacag cgttatgcat
     4921 catctttcaa ggcttgataa agcctttctg cgcaaaggga tccaactttc ccgcgccaac
     4981 aatattgttt cattatctcg tccatcaggt gccagtgccc gcgtactgct gccggacgaa
     5041 ctgccgctgg aagaaaaagc agtaacacaa ctgcttgatt ttgcagaagt gaagctacca
     5101 gaccacccag gttatgtgcg ccagacgtgc gcgacgccag attttcatcc cggcaatggc
     5161 gtccccgttg gcgcggtggt ggcctcaacg ccggatttag tcattcccgc cgccatcggc
     5221 accgatattt cttgtggcat gcggctgctg acgaccggat taacacttga gcaagccgaa
     5281 gcgcataaag acgtcatcgc tcagcggtta acgcaagtgc tactgcaaaa tgagcgcgat
     5341 gttccgttat ccgcacaggc gttcaccgcg ctttttgatg gcggaccggc agattgcgtg
     5401 tcgaccttgc catcagaagg tctctggata cgcacgaata tcccccgaat ggtacaagag
     5461 attgaccgct gcattgggct taacgagcat aacagtcatt cgcgttatgc cccacccgcc
     5521 ctgcaggcaa cggaccgcgc attactccgc gatccttgtc tgggtacgcc gggttcaggt
     5581 aatcatttta ttgaacttca ggtcgttgat agcgtggttg atcgccatgt agcctggcaa
     5641 caagggctga aaacaggcga tattgtggtg atgatccaca gtggatcgcg agatgttggc
     5701 ttttacgtcg gaagacactg gatggatcgc gcccgtgctg aatggcccac cggacataaa
     5761 catccacaaa gcgggttata tggtctggca ggcggactgg cggaggaata tctacaagcg
     5821 ataggaatgg cggcgcgtta cgcatggtta aatcgcgtca caattgccga aatggtgcgc
     5881 gtatgtcttg ccaatctctt tcatcaggac aattcgcatc tggtggtgga tgtgccacat
     5941 aacatcatcc ttcgcgagca tgaaatgaat attcatcgca aaggcgcaac accggcacga
     6001 gtgggcaatc tggcgttaat ccccggttca atgggcgatt attcgtggct tgcggtaggt
     6061 tgcggcaacc cggagtggct gtggtcatgc agtcacggag caggacgcag ccagcgtcgg
     6121 caggccatgc gctcacgcgc gactgaagag tcaacgctgc cgtggcaatg cgtcacattg
     6181 cgcgaagaac gacgttttga agaagcgcca gcggcttata aagatattgg tccggttatc
     6241 gaagcccaac aagaagccgg attaattcag ccagcggtca gatttcgacc gtggttaacg
     6301 tttaaggggt aactgaaatg ataaatggcc tgcggttctc acgcaggtca aatctggtat
     6361 ctgcgtaagc taacaaactt gtttatcaac aatctcaggc catccgaaga tggcctgaaa
     6421 ttattgcccc cctcagatac acttttagcg caataatcag ccaaggcggt agctaaatgt
     6481 ccgtgactgc cccggtaaaa atgcccccag ttcctttaaa ttatctcttt ccgctgtgct
     6541 gatgctttca tccatcatcg tttgcgtaca tgaaatcacc tcacgactga acgcgacagt
     6601 tgcctcacag gttgtcgatt ctgccggatt aaacagtcgc aataccactt cttgccggtc
     6661 ttccgctttt ttaagagcgc taatcaggca tcccacaggg ggcattttca gcaagctata
     6721 gctttccggc acattgaatc cggctttatt taacttcatc gcatcccacg ggattttgtt
     6781 gtaacactgt actggcgtca gccatgcgcg cgcctgctgg gctacaccgg cggtcgaagg
     6841 cgtacctgta taactaaata agctcagacg gcagctaaag gagccacgta attgtgaatc
     6901 gggcaccggc atttttattc ctgaaggacg tccaggcctt aaaagcagat cttctttccc
     6961 cagtaagccc acgccacgca gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac
     7021 ttcatattca cgtagccctt cgctaaagac agccagaccg ttacgccctt cctgtaaggc
     7081 agcatagttg agcatattcc ataccggaac cggcgcttct ttccagcctt cttgctgcca
     7141 gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg tatcggccag
     7201 aacactgtcg gtgttaaaag gtgtagggac caggacgcga aggcgatgat cgtcagcctg
     7261 gttatcaagg ttgatatcca catcaatgcg cctgctgtta tcactcagag tgacagccaa
     7321 ctccacccct actctgccag tggattgtct tgcgctgcgt tcatgcagat tacgcggcac
     7381 cgccatgtca tagcggataa cagccctact ctgccaggcc tcatgagtaa tctcgcattg
     7441 cggtttcgcg ttcgccgaag taattaccca ctcttctttt gcgggtgaat agtcatattc
     7501 atcaccatca tcagagcttt cttcaatttg caacacccgg tcatagcgca caccgctgtc
     7561 tttatctacc agttgtagcg ttccatcctc attgagcgca atttgccaga aagcattttc
     7621 cagtatccct tcagcgtcac tttttgccgc aatcacgttg ccaggctgat tcgcttcgat
     7681 ataaagcgtg cgatagccca tagaagggac aatctggttg atctgaatat caaactccat
     7741 aaagggatca taattaccgt aatgaacgat ttgccgatcg attaggccag gatcgatctc
     7801 acgggcatgg cgaataaaat acggtacagg ctgaccgcga tcgtcccgca aattaaactg
     7861 gctggcgcgc agccgcacag tggtgttgat cacttcttcc cgcggccagg gcatcaggtt
     7921 aaacagcaca agtttgtcgg catcactctg tggcatgtta tcggcaattt tacgcatata
     7981 gaaacgcagc agattatccg ccatgtcttc tgccagttcg aaacgggcga caatctcgcg
     8041 atgaactttg tcactacagc agcagccgat gctgtcatgg gcgtggtttt ttaaaatttc
     8101 tttccacaat ttctccagca agccgtggtg gtattcaaaa cccaacgtcc aggccagcgt
     8161 tgccagcggt tccagcagat taacaatctt attttcaata cgcgcgtggg caattttgat
     8221 atccatacgc gtagaaccga tggtgcgatg cacgcgcata tatttgccat caataaattc
     8281 ccctttcagg gttgccagat tatctcgctg cgcttcgatc ttctcaaata cctcttcaaa
     8341 gcggctcatc acaaacttac gttgagggta gatctcacgt agcttatcca tcacttcgaa
     8401 gatattttgc tgcaatggca tctggtcatg accattcggc agcaaaatct ctttggttac
     8461 tgacgctttt tccagcacat caaaataact gtcgaggcgt ttgcgtaatc cgtcttcgtc
     8521 ggcaggtaag tacttaccga tggcgtagcc cagcggcagc acctgcgccg tcacttcgct
     8581 accgtcactg ctttgccaca aaaactcggt tttatcagta ccgtggcgct cagaacatcc
     8641 gcgccagaac atggtgcggg taatgccaaa tccattgtaa atatgcggca gttgcccgga
     8701 catgccaaag gaatctggta aataacctat tttcatcggc tcgccaaacg tgaggcagtc
     8761 acgcattccg tacatcagat tacggacgat ggattccgca gaaacaatcg tggtatcggt
     8821 ctgggtatac caggggccga taatcaactt gcctgcttct acctgtttct tcacacggtc
     8881 tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc gccgtttgcc cgtcgagtac
     8941 gtaatatttg tattcgttgt cctgttccag tcggcacagg atctcttcca tattattgac
     9001 cagcagaatg cgtgactctt cggtggtgaa ataccactcc cgatcccagt gcatatgcgg
     9061 ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc tttttgttta tggcattacg
     9121 ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc gccagatcag aagaattgca
     9181 gtcgagattg cagcccccac caatgccgca ccaaaccagc caattgccgc cataacaccg
     9241 cccgcgccat tatcatgaag taaaaagagc gagaaaatgc cggcaccggg tgtcgaaagg
     9301 ccgatattca tcgcgccgac gatagcgccc gttaccatag agcccagcac aaacgaacca
     9361 ataacccgca gcggatcttc aatcgccatc gggatcgccc cttcggtaat acctgccagc
     9421 cctaacagcc aggtggattt cccggtctca atttcaaact ctttaaacag gcgcggtgcg
     9481 agcatcgtgg aagcggttac ggtaaatgcc gaaaccattt tgacggaggc gaaaatggca
     9541 tacgggccgt aaacgccgtt cgccattgcg cccaggcaga atgcataagc ggctttgttc
     9601 actggccctc caaggtcaaa ggaacacata aaaccgagaa tggcacccag caacagcgcg
     9661 ttacttcctg acagaccgtt cagccaggcg gtaagcgagt tattgatcca ggcgacaggt
     9721 tcccccacca caaacagcat cagactgccc gctcccaacg taccgagcac cgggtagaga
     9781 taaaaagtca ggaatccatt gaatttactg ctaagacgca agtgattttt cacccagcgc
     9841 atcagataac cggctatcaa tccgccaacg acagcaccga gaaacccgga accgatcatg
     9901 ttggcggcaa gtccagccgc aaagcctggc gctaacgccg gtttatcagc cagtgaatag
     9961 gcggtgtagg ctgccagcac cggtaccatc aaaatcccga gcagccctcc gcccagtttg
    10021 cggtacatcc acagccagga gttttcttca ttaaacaaat cttgtagccc gaagatttgc
    10081 gacagtaata ccgcaaccgc cagcaccgtg ccccccgcga caatcaacgg cactgcaaaa
    10141 gagatcccgc tcaacagtgc ctgttttagc tccgttttga ggcttttcac cggttgcgta
    10201 tcttgctgta cggtacgcgt ctcatcgcta cgcttgaggt taagcgcttg ttggatcaac
    10261 gcttctgcat ggcgaatcgg ctcggcaaca ggcacagaaa gcgcgggaat gccgttaaaa
    10321 cgctcactct ccttgatggc gacttcagcc gcaaaaatac aggcggttgc actattgagt
    10381 tgctctgccg ttaaacgccc ttcaatgccg ttagcacctt gcttttcaac gtagacattc
    10441 acgccgagtt tgcgtccggc tttttccaga tactccgcag ccatataggt gtgagcaata
    10501 cccgccgggc aggccgttac gcagacgata gttggcgcgt tggaaaaaga agctgagggt
    10561 tgcgtgcctc ccttgtcatc cagcgccgag agcaattcat caggcgtcgt cgccgactgt
    10621 atacgcgccc gtatctcatc atccgccagg cgcgtggtca gcgctgtcag cagttgcatg
    10681 tgcgtagtac ccgcttcatt ggggggaatc gccagcaaca ccactaaatc aaccgcttcc
    10741 gggccatcca cgccttccca ctgaagcggc tcgctgagtg tggcgaccgc aaacgccgct
    10801 tcttttaccg cagcagtttt gccatgcggc acagccagcc cttcacctaa ggcggtcggg
    10861 ccaaggcttt cacggcgata cacttctttc agaaattgct cagtactgga aattttcccc
    10921 agagcagcaa gacgttgagt caacgcgtgg atggcctctt cacggctggt aaagcgcgca
    10981 ttcagacaca acgcatcgcg gtgggtcaga gtcgtcaggt tcatcatgat cctcacttga
    11041 tcgtttttat agtcgcgcca gtgtgcccga taaaacaata ccattatgtg atcaatttca
    11101 cttgattgaa attatttgta ttgtatttgt attatttaca ggatgaattt atcgggcata
    11161 atggctaaat tcgtattaat gagatgctaa atcatgggac ggaagccctt ataccgacag
    11221 attgctgatc gcattcgtga gcaaattgcg cgtggcgagc ttaaacctgg cgatgcgtta
    11281 cccactgaat cagcgctaca aacagagttt ggcgtcagcc gggttacggt gcgtcaggct
    11341 ttgcgccagt tagttgaaca gcagatcctc gaaagtattc agggcagcgg gacttacgta
    11401 aaagaagagc gggtcaatta cgatattttt cagttaacca gttttgatga aaaactgacg
    11461 gaccgtcacg tcgatacaca cagtgaagtt ctgatattcg aagtgattcc ggctgacgat
    11521 tttcttcagc aacagctaca aatcaccgcg caggatcgtg tgtggcatgt gaagcgcgtg
    11581 cgctatcgca agcaaaagcc aatggcgctg gaagaaacct ggatgccgct tgcgttgttc
    11641 ccggatctca cctggcaggt catggaaaat tcgaaatatc actttattga agaagtgaag
    11701 aaaatggtta tcgatcgtag cgaacaggaa atcattcctc tgatgccaac cgaagaaatg
    11761 agccgtctgc ttaatatcag ccagactaag ccgattcttg aaaaagtatc gcgtggatat
    11821 ttagttgatg gccgggtgtt tgaatatagc cgcaacgctt ttaataccga tgactataaa
    11881 tttaccctga tagcgcagcg aaaatcatcg cgataagcat aaaaaaggcc atcatacgat
    11941 ggccaaccat gtcgaaaccg acagcccttc gccattcctg tagaaggaag cggcgagggc
    12001 tatttcttat tacagatatt tatttcagaa cagttttcag tgcttcaccg atatccgcca
    12061 ggctgcgaac ggttttcacg cctgcggctt ccagagcagc gaatttctca tccgcagtcc
    12121 ctttcccacc ggcaatgatc gcacccgcgt ggcccatacg tttgcctttc ggcgcagtca
    12181 caccagcgat gtaacctaca actggcttgg taacgtgctc tttgatgtac gccgctgctt
    12241 cttcttcagc gctaccgccg atctcaccga tcatcacgat cgcttcggtc tgcggatctt
    12301 tttcgaacat ttcgaggatg tcgataaagt tagagccagg gatcggatcg ccgccgatac
    12361 caacacaggt cgactgaccg aaaccgtaat ccgtggtctg tttaaccgct tcataggtca
    12421 gcgtaccgga acgggaaacg atacccactt tacccggttt gtgaatgtga ccaggctgga
    12481 taccgatttt gcattcaccc ggagtgataa cgcctgggca gttcgggccg atcatacgaa
    12541 cgcctgcttc atccagcttc actttcacgg tcagcatatc cagcgtcggg atgccttcag
    12601 tgatggtgat aatcagtttg atgcctgcat cgatagcttc cagaatggag tctttgcaga
    12661 acggtgctgg tacgtagata acagaagcgg tagcgccagt agcagcaaca gcttcacgca
    12721 cggtgttgaa caccggcagg ccgaggtggg tggtgccgcc tttacctggg gttacgccgc
    12781 caaccatttt agtgccgtat gcaatggctt gttcagagtg gaaagtcccc tggctaccgg
    12841 taaagccctg gcagataacc ttggtgtttt tatcaattaa aatggacatt atttcccctc
    12901 cactgcggca acaacctgct gagctgcatc cgtcagacct tttgctgcaa taatattcag
    12961 gccgctgtca gccagtttct tcgcgccgag ttcggcgttg ttaccttcca gacgtaccac
    13021 gaccggtacg ttaacaccca cttccgctac cgcgccgatg ataccgtcag cgatcaggtc
    13081 gcaacgaacg ataccgccga agatgttaac cagaacggct ttcactttgt cgtcagagag
    13141 gatgattttg aacgcttcgg ttacacgttc tttggttgcg ccgccgccaa cgtccaggaa
    13201 gttagctggt tcgccgccgt gcagtttaac gatgtccatg gtgcccatcg ccaggcctgc
    13261 gccgttaacc atacaaccga tgttaccgtc cagcgcaacg tagttcagtt cccactgtgc
    13321 agcctgtgct tcacgcggat cttcctgcga ctggtcacgc atttcgcgca gatcaggctg
    13381 gcggaacagt gcgttgccat cagcgcccag tttgccgtcg aggcaaatca gatcgccctg
    13441 tttggtaatg accagcgggt tgatttcgat cagcgccagg tcgcgctcca ggaaaatggt
    13501 cgccaggccc atgaagattt tggtgaactg ctgaaccagt ttaccttcca gacccagttt
    13561 gaacgccagc tcgcgtccct gatacggcat cgggccagtc agcggatcaa gcgcaacttt
    13621 gtggatcagg tgcggagttt cttccgccac tttttcgatt tccacgccgc cttcggtgga
    13681 ggccataaag accacacgac gggaactacg gtcaacaacg gcaccgagat acagctcttt
    13741 agcgatatcg gtcgctgctt caaccagaat ctggttaacc ggttggccat tggcatctgt
    13801 ttgatacgtt accagacgct tgcccagcca gttttctgca aaagcacgga tgtcttcttt
    13861 gctgtttaca actttcacac cgcccgcttt accgcggcca ccagcgtgaa cctgacattt
    13921 cactacccac ggaccggcac cgatttttga agcggcttct tctgcttcgc gcggagtagt
    13981 acaggcataa cccaccggtg ctggtaagcc atagcgggca aaaagttgtt ttgcctgata
    14041 ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc gttatctttt aaaccgtaga
    14101 ccgacaccct atccacgtcg gtctacagtg caggtgaagc ttaaactact acacgtccag
    14161 cagcagacgc gtcggatctt ccagcaactc tttgatcgtt accaggaagc ccacggattc
    14221 gcgaccatcg atcagacggt gatcgtagga cagcgccagg tacatcatcg gcaggatctc
    14281 aacctgacca ttcaccgcca tcggacgatc tttgatagcg tgcataccca gaattgcgct
    14341 ctgcggtggg ttgatgatag gcgttgacat cagggaaccg aacacaccac cgttggtgat
    14401 ggtgaagtta ccaccagtca gatcttcaac cgtcagcttg ccgtcacggc ctttaacagc
    14461 cagctctttg attttcttct cgatgtctgc catgccgagg gtatcgacat cacgcagaac
    14521 cggcgtcacc aggccgcgcg gcgtagaaac cgccatactg acgtcgaaat agttgtggta
    14581 gaccacatca tcaccgtcga tagacgcatt cacttccggg taacgtttca gggcttcaac
    14641 caccgctttc acgtagaagg acataaagcc cagacggatg ccgtggcgtt tttcaaacgc
    14701 ttcaccgtac tgcttacgca gatccataat cggcttcatg ttgacttcgt tgaacgtggt
    14761 cagcatggcg gtggagtttt tcgcttccag cagacgctct gccacacgct tacgcaggcg
    14821 agtcatcggc acacgttttt cgctacgtgc agccagggcc ggttgcgccg ccggagcagg
    14881 cgctgccgga gcagactctt tcaccggggc tttcgccaga tgtttttcca catcttcacg
    14941 ggtcagacga ccacccacac cggtgccttt aatggcgctg gcgtcgaggt tatgttcagc
    15001 cagcaggcga cggatcgccg ggcttaacgc atcgttgttt tgctcttcca gagacgcctg
    15061 ctggcgttgc gccggagtgg acgctttctc ttcagatttg gcgctggttt ctttacccgc
    15121 gctgttgcct tcacgcaggc gaccaaggat ctgacgagac gttaccgttg taccttcatc
    15181 ttccagaacc gcatccagaa tgccgtctgc tgatgccggt acttccagta ccactttgtc
    15241 agtttcgatt tctaccagca cttcatcacg tacgactgcg tcgccgggtt ttttatgcca
    15301 ggttgcgacg gtggcatcgg ctacggattc aggcaggtca gggaccagaa tatctacgct
    15361 actcattgtg tatcctttat ttattcgacg ttcagcgcgt cattaaccag atcttgttgc
    15421 tgtttctggt gaacggacat ataccctacc gccggagagg cggaggccgg gcggcctgca
    15481 taacgcagag aagccccaaa cggaatcact tcacggaaat gatgctggct gcagtaccat
    15541 gcgccctggt tgagcggctc ttcctggcac cagacaaaat ccttgacgtg agcaaactgc
    15601 tgcaacactt cctgcatcgc tttatgcggg aacgggtaga gttgctcgat acgcacaatg
    15661 gcgacatcgt gttgattgtt cttacgacgt tgttccagca ggtcgtaata aaccttacca
    15721 gaacacatca ctacgcgctt cacgcccttc ggatcaagct cgtcgatttc accgatggct
    15781 ggcaggaagg tgccgttcgc cagttcttcg aggctggata ccgccagcgg atgacgcagc
    15841 agggatttcg gcgacatcac gaccagtgga cgacgcatcc cgcgcaacgc ctgacgacgc
    15901 agcatgtggt aaacctgtgc cggggtagac ggtacgcaaa cctgcatgtt ttgctcagcg
    15961 caaagttgca gataacgttc cagacgcgcg gaggagtgct ccggcccctg cccttcgtaa
    16021 ccgtgcggca gcaacatcac caggccacac atccggcccc atttctgttc gccagaggag
    16081 atgaactggt cgataaccac ctgcgcaccg ttggcgaagt caccgaactg cgcttcccag
    16141 atggtcagag tgcgtggttc tgcggtggca taaccgtatt caaacgccag cacggcttct
    16201 tctgacagta cggagtccca gacacggaac gcgccctgac cgttatggat gtgttgcagc
    16261 ggcgtgtaag tggaaccgtt agactggttg tggatcaccg cgtggcggtg gaagaaggta
    16321 ccgcgaccgg agtcttcacc cgacaggcga accggaatgc cttcatcaac cagcgtggcg
    16381 taagcgaggt tttccgcacc gccccagtcg aacagtttct caccggctgc cattgcctgg
    16441 cgatcgccat aaatcttggc aacgcgagac tgcatttcaa ctgcttccgg caccgtgctg
    16501 atgcgtttag ccagttcctg caggcgcttc atctcaactt tgttcgggta ctcttcgtcc
    16561 cattcgtggt tgaggtacgg cgaccaggtg aaagagtgca tgttcatcgg acgccactct
    16621 gctacaacgc aatcgccagc atccagcgca tcgcggtaca ggttaaccat ctcggtggca
    16681 tcttccagcg tcgcgacttt ttcctgctcc agcttgtcag catagatttt gcgcggcgtc
    16741 ggatgttttt tgattttctg atacatcagc ggctgggttg cgctcggctc gtcggcttcg
    16801 ttgtggccgt gacggcggta gcacaccaga tcgatgaaga catcacgttt aaaggtgtta
    16861 cggaaatcga gcgccagacg ggtcacaaag gcaacggctt ccggatcatc cgcattaacg
    16921 tggaaaatcg gtgcctgtac catcttgccg atatcagtac agtacggcgt cgaacgggca
    16981 tccagcgggt tagaggtggt gaagccaacc tggttgttga taacgatacg taccgtaccg
    17041 ccaacttcat aaccacgcgc tttcgacatg ttcagggttt cctgaaccac gccctgcccg
    17101 gtcactgcgg cgtcaccatg aatggtgatt ggcagcactt tgttgctgct cggctcatca
    17161 agtctgtcca gacgggcacg aacagaaccg ataacgaccg ggctgacaat ctcaaggtga
    17221 gacgggttaa acgccagcgc caggtgcacc aggccgccat cggtctggaa gtcagacgag
    17281 aagcccatgt ggtatttcac gtcgcccgtg ccgaggtgtt ctttatgttt accggcaaac
    17341 tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca ccagcacgtt cagacgacca
    17401 cggtgcgcca tcccgagaac cacttcgcgg gtgccgctgt tgccagcgtg gcggatcatc
    17461 tctttaagca tcgggattaa cgcgtcacca ccttccagcg agaagcgttt tgcgccaggg
    17521 aattttgcgc cgaggtaacg ttcaaggcct tcagcggcgg tcagttcgct taagaagcgt
    17581 tttttctctt cgctattgaa agtcgcgcga ccagactcga tacgctgttg gatccagcgt
    17641 ttttcttcag tgctggtaat gtgcatatac tcggcaccaa tcgggccgca gtaggtttgc
    17701 ttgagggctt ccagcagctc gccgagtttc atggtttctt tgccgctggc aaatgaaccg
    17761 acgttgaagg tctcctggaa gtctgcttcg gtcagatcgt ggaaagacgg atccagatcg
    17821 gccactttat cttgctgcca cagtcccagc ggatcgagat tcgcatgctg gtgaccacgg
    17881 aagcggtatg cgttaatgag ctgcaggact ttaacctgct tcacattggt gtcagggtcg
    17941 gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc ggcggaaata ttcacgcgtt
    18001 tgagagtgga attgatccgg tttgactccc gtaccaggta actgctggaa cgtcgaacgc
    18061 cagttagcgt caaccgagtc aggatcggtt aagaagtctt catagagctg ttctatccag
    18121 ctctggtttg cgccagagag gtaagaagag tccaaccagg ctttcaaagc gctgttctgc
    18181 atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc gtggatacta ccacgcacag
    18241 tgatgtgcgt gccggggttc acttgcgagc tcttgtctac gtagtggctc gcgaaggaac
    18301 ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga tttcctgcat ttctttgtta
    18361 cgcctgatgc gctgcgctta tcaggcctac gggtaatgac ttgtaggccg gataaggcgt
    18421 tcacgccgca tccggcactg gttgcctgat gcgacgcttg cgcgtcttct caggcctacg
    18481 gtttacgcat tacgttgcaa caacatcgac ttgatatggc cgatggcgcg cgtcgggttc
    18541 agccccttcg gacatacact gacgcagttc atgatgctgt gacagcggaa tacactgaat
    18601 gcatcgctca aaccgtcgag gcggctgtca gtctcggtat cacggctatc gatcaggaaa
    18661 cgatatgccg ctaacaagcc tgccgggccg ataaacttat cgggattcca ccagaaagac
    18721 ggacaagagg ttgaacaaca tgcgcagaga atacattcat acaacccgtc gagtttttcg
    18781 cgctgctctg gcatctgtaa atgctcgcga gctggcggat tttgtccatt attcaacagg
    18841 taaggcttaa ttttctcata ttgcgcatag aattgtccca tgtctaccac caaatcgcgg
    18901 atcaccggta aacctggcag cgggcgaatc acaatcttct tgcccggctg gttgagtgcc
    18961 gaaatcgggg taatacaggc cagaccattc ttgccgttca tgttcagacc gtcggaacca
    19021 cacacacctt cacggcagga gcgacggaac gacaggctgg gatctttctc tttcagctgg
    19081 ataagcgcat ccagcagcat catgtcacga ccttcttccg cttccagggt gtaatcctgc
    19141 atacgcggag catcatcaac atccgggtta tagcgataaa ttgaaaactc gagtctcatt
    19201 ttcctgtctc cgcattagta agtacgaatc ttcggcggga atgccgggcg cagtttcggt
    19261 tccatgttga cgcttcggcg cgtcatggat tccgactcag gcagatacag ggagtggcac
    19321 agccagtttt catcatcacg atccgggaag tcgaagcggc tatgtgcgcc acggctttcg
    19381 gtacggaagt tggcagaaac agccgttgca tacgccgttt ccatcaggtt atccagttcc
    19441 aggcactcaa cgcgctgggt attgaactca cttgaagtgt catccagacg ggcatttttc
    19501 agacgctcgc ggataacttt caactgctca agccctttcg ccatcgcatc accttcacgg
    19561 aagaccgaga agttatgctg catacattct tgcagtgctt tacggatcgc caccggatct
    19621 tcaccgttac ggttattgtt ccagcggttc aggcgatcca gagacgcttc tacatcagac
    19681 gcgctggcat cgcgcagtgc gccctgctcg gcgatagact cttgcagatg cagacctgcc
    19741 gcacgaccaa atacgaccag gtccagcagc gagttgccgc ccagacggtt agcgccatgt
    19801 accgatacac aagcgatttc accaacggca aatagccccg gaacaaccac atcttcgcct
    19861 ttctcgttca ccgtcagcgc ctgaccggtc actttggtcg gaataccgcc catcatgtag
    19921 tgacaggttg ggataaccgg aatcggctct ttcaccggat caacgtgagc gaaggtgcgg
    19981 gagagttcaa ggatacccgg cagacgggat tcaagaactt ctttacccag atggtcaagt
    20041 ttcagtttcg cgtgtggccc ccacggacca tcacagccgc gaccttcacg gatttcaatc
    20101 atgatggaac gcgccaccac gtcacggccc gccaggtctt tggcgttcgg tgcataacgc
    20161 tccataaagc gttcgccatg tttgttcagc agataaccgc cttcaccacg gcacccttcg
    20221 gtgaccagta cgcccgcacc ggcaatacca gtcgggtgga actgccacat ttccatatcc
    20281 tgcactggta cgcctgcacg gatagccatg ccgacgccgt cgccagtgtt aatgtgggcg
    20341 ttggtagtgg actgataaat acgccctgcc ccgccagtcg ccagcacggt agcgcgagct
    20401 ttgaagtaaa ccacttcacc agtttcgatg cacagtgcgg tacaaccgac caccgcgcca
    20461 tcctggtttt tcaccagatc cagcgcatac cactcggaga aaatggtggt gtggtttttc
    20521 aggttctgtt gataaagggt gtgcaacagt gcgtgaccgg tacggtcagc tgccgccgca
    20581 gtacgtgccg cctgctcgcc gccgaagttt ttcgactgac cgccaaacgg acgttgatag
    20641 atacgaccat catcaagacg cgagaacggc aggcccatat gttccagttc cagaatcgct
    20701 tccggcccgg ttttacacat atattcaatc gcgtcctggt caccgatata gtccgaacct
    20761 tttacggtgt cgtacatatg ccattcccag ttatcttcat gggtattacc cagcgcaacg
    20821 gtaatgccgc cttgcgcaga aacggtatgg gaacgggtcg ggaagacttt agagagcagc
    20881 gcacaggtct gaccgctctg ggaaatttgc agcgccgcgc gcatacctgc gccaccggca
    20941 ccaattacaa ctgcatcaaa ttctctgact ggcaatttca tcacacaccc cacaccacaa
    21001 cgaatccata aatcacgtaa accaccagag caacgacaat caccagttgc agcatcaggc
    21061 gcaaggccag cggtttaacg tagtcggtca acacctgcca catgccgatc caggcatgga
    21121 tcaagataga aaacagcgcc agcagggtga acactttggt gaacgcagag gcgaagaaac
    21181 cgatccagac ttcatatgtc agctcgccac tggtagcgaa aaaaccgacc atataaatga
    21241 tgtagagcgt caggacgata gcggtagcgc gaacgaggat gaaatcatgt acgccattgc
    21301 gtcctaatgc ggaggcgttg cttaccatac gaggactcct gcgagaagtg aaagcacgac
    21361 agtaataaca aaggatattt tggcggagcg tttacccgct tcgaatgttt cttccagata
    21421 gccaaaatcc atcatcatgt ggcgaatacc tacgacgacg tgatacgcca gagcggtaag
    21481 gatgccccac atgataaatt tgacgaagaa gctgcccata atcgcggaag cttgctcgaa
    21541 accttcaggg gaagagaggc tggtacccag aagccacagc aggatgccca ctgcaacaaa
    21601 ggtgatcaca ccggaaacgc gatggagaat ggacgctatc gccgtgatgg ggaaccggat
    21661 ggtctgtagg tccagattaa caggtctttg ttttttcaca tttcttatca tgaataacgc
    21721 ccacatgctg ttcttattat tccctgggga ctacgggcac agaggttaac tttctgttac
    21781 ctggagacgt cgggatttcc ttcctccggt ctgcttgcgg gtcagacagc gtcctttcta
    21841 taactgcgcg tcatgcaaaa cactgcttcc agatgcgaaa acgacacgtt acaacgctgg
    21901 gtggctcggg attgcagggt gttccggaga cctggcggca gtataggccg ttcacaaaat
    21961 cattacaatt aacctacata tagtttgtcg ggttttatcc tgaacagtga tccaggtcac
    22021 gataacaaca tttatttaat ttttaatcat ctaatttgac aatcattcaa caaagttgtt
    22081 acaaacatta ccaggaaaag catataatgc gtaaaagtta tgaagtcggt atttcaccta
    22141 agattaactt atgtaacagt gtggaagtat tgaccaattc attcgggaca gttattagtg
    22201 gtagacaagt ttaataattc ggattgctaa gtacttgatt cgccatttat tcgtcatcaa
    22261 tggatccttt acctgcaagc gcccagagct ctgtacccag gttttcccct ctttcacaga
    22321 gcggcgagcc aaataacaaa cgggtaaagc caggttgatg tgcgaaggca aatttaagtt
    22381 ccggcagtct tacgcaataa ggcgctaagg agaccttaaa tggctgatac aaaagcaaaa
    22441 ctcaccctca acggggatac agctgttgaa ctggatgtgc tgaaaggcac gctgggtcaa
    22501 gatgttattg atatccgtac tctcggttca aaaggtgtat tcacctttga cccaggcttc
    22561 acttcaaccg catcctgcga atctaaaatt acttttattg atggtgatga aggtattttg
    22621 ctgcaccgcg gtttcccgat cgatcagctg gcgaccgatt ctaactacct ggaagtttgt
    22681 tacatcctgc tgaatggtga aaaaccgact caggaacagt atgacgaatt taaaactacg
    22741 gtgacccgtc ataccatgat ccacgagcag attacccgtc tgttccatgc tttccgtcgc
    22801 gactcgcatc caatggcagt catgtgtggt attaccggcg cgctggcggc gttctatcac
    22861 gactcgctgg atgttaacaa tcctcgtcac cgtgaaattg ccgcgttccg cctgctgtcg
    22921 aaaatgccga ccatggccgc gatgtgttac aagtattcca ttggtcagcc atttgtttac
    22981 ccgcgcaacg atctctccta cgccggtaac ttcctgaata tgatgttctc tacgccgtgc
    23041 gaaccgtatg aagttaatcc gattctggaa cgtgctatgg accgtattct gatcctgcac
    23101 gctgaccatg aacagaacgc ctccacctcc accgtgcgta ccgctggctc ttcgggggcg
    23161 aacccgtttg cctgtatcgc agcaggtatt gcttcactgt ggggacctgc gcacggtggt
    23221 gctaacgaag cggcgctgaa aatgctggaa gaaatcagct ccgttaaaca cattccggaa
    23281 tttgttcgtc gtgcgaaaga taaaaatgat tctttccgcc tgatgggctt cggtcaccgc
    23341 gtgtacaaaa attacgaccc gcgcgccacc gtaatgcgtg aaacctgcca tgaagtgctg
    23401 aaagagctgg gtaccaaaga tgacctgctg gaagtggcta tggagctgga aaacatcgcg
    23461 ctgaacgacc cgtactttat cgagaagaaa ctctatccga acgtcgattt ctactctggt
    23521 atcatcctga aagcgatggg tattccgtct tctatgttca ccgtgatctt cgctatggca
    23581 cgcaccgttg gctggatcgc ccactggagc gaaatgcaca gtgacggtat gaagattgcc
    23641 cgtccgcgtc aactgtatac aggatatgaa aaacgcgact ttaaaagcga catcaagcgt
    23701 taatggttga ttgctaagcc gtttactttc cggacccgcc tttaatagag cgggttcttt
    23761 tttatatggc tgaacataat aaattgtaaa aattaaacca gatgcaaata cccattaata
    23821 aggtgaaaaa atgtgacaag tcacatactg agcagaatga taagtttatt tttttacttt
    23881 cctgccggta gcattgttaa cccaatgaaa attatgcata tccatcccac cccatctttc
    23941 atctcattaa acaataaatt tatgcactca attattccat tctttatata acaagctttt
    24001 taatattgtt aaataaaacc aaatacattc aactgtcagt taatatattc ttgcctcgca
    24061 acactctatc tcattattta aaggactcag ctatgtttaa aggacaaaaa acattggccg
    24121 cactggccat atctctgcta ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg
    24181 aaattcactt taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg
    24241 ataaagaggt tgaactcggt caggtgacca ccagccacat taatcagtcg catcacagcg
    24301 atgccgttgc tgtcgacctg cgcttagtca actgcgatct ggaaaactcc aacaacggtt
    24361 ccggcggcaa gatttctaaa gttgcagtca cctttgacag ctcagcgaaa accaccggcg
    24421 cagatccgat tctcaacaac accagcacgg gtgaagcgac cggtgtcggc gtgcgtttaa
    24481 tgaataaaga tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggcac
    24541 caacctccag cgaacaaacg ttaaatttct ttgcgtggat ggaacaaatt gatcaggcta
    24601 cgcccgtaac accaggcgca gttacggcga atgcgaccta cgtactggat tataaataag
    24661 tatcactttg gcggcgtcat cgtcgccttt cttcaaatat ttatcaggta tggacaccgt
    24721 gaatatttat cgactctctt ttatatcctg tctggtcatg gcgatgcctt gcgcattggc
    24781 ggtcgaattc aacctgaatg tcctcgataa atcgatgcga gatcgcattg atatttcatt
    24841 attaaaggga aaaggggtca ttgttcccgg tgaatatttt gttagcgttg cagtaaataa
    24901 caaccaaatc agtaacggac aaaaaattga ctggaaaaag aacggtgacc aaaccattcc
    24961 gtgtattaat gatttactgg tagataaatt tggtttaaaa cctgaagttc gtcagtcgtt
    25021 accacggtta aaccagtgcg tcgattttag ctcccggcct gaaattctct tcatttttga
    25081 tcaggctagc cagcaactga atatcactat tccacaagcc tggctggcgt ggcactcaga
    25141 taactggact cccccctcaa catggaaaga aggtgtcgcc ggcgtcctga tggactacaa
    25201 cctgtttgca agcagctacc gcccacagga tggtagcaac agcactaacc tgaacgccta
    25261 tggaacagcc ggaattaacg cgggagcatg gcgtttacgc agtgattacc aattgaatca
    25321 gaccgatagc gatgataacc atgaacagtc aggcgaaata tcacgcacct atctttttcg
    25381 tccattaccg caattaggtt caaaattaac ccttggtgaa acggatttca gttccaatat
    25441 tttcgacggc ttttcttata ccggcgcggc actggcaagt gacgatcgga tgttaccgtg
    25501 ggagctacgc ggctacgccc cacaaatcag cggcattgca caaaccaacg ccaccgtcac
    25561 cgtgagtcac tcagggcgag ttatttacca gaagaaagtc ccaccaggtc cgtttatcat
    25621 tgatgacctt aatcagtccg tccagggtac gttagatgtc aaagtgacgg aagaagatgg
    25681 tcgggcgaac aatttccagg tttcggcagc atcgacgcct ttccttacgc gccaggggca
    25741 ggttcgctat aaacttgctg ccggtcagcc gcgaacatct atgtctcatc aaactgaaaa
    25801 tgaaaccttt tttagcaatg aagtttcctg ggggatgctg tcaaatacct cactgtacgg
    25861 cggcctgctg atttcgggtg acgactacca ttctgccgca atgggtatcg ggcaaaatat
    25921 gctgtggctt ggtgcattgt cgtttgatgt cacgtgggcc agtagtcagt ttgatactca
    25981 gcaggacgag cggggcttaa gttaccgctt taattacagc aaacaagtgg atgccaccga
    26041 cagcacgatt tcgctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa
    26101 ctatctggat cacaaataca acaacagcga tgcgcaggac gaaaaacaga cgatcagctt
    26161 atccgtgggc caaccgatta ccccactgaa cctcaatctg tacgccaacc tgctgcatca
    26221 aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcgggtt ttaatgttga
    26281 tattggtaac tggagagata tctcgatctc gacgtcattc aatacaaccc attacgaaga
    26341 taaagatcgc gacaatcaga tttacctgtc gatttcgctc cccttcggta acggggggcg
    26401 ggtcggttat gacatgcaaa acagtagtca cagcaccacg caccgcatgt cgtggaatga
    26461 tacgttagat gaacgtaata gctggggcat gtctaccgga ctgcaatccg accgtccgga
    26521 taatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat
    26581 ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt
    26641 cacggcaact caatatggtg cggcgttcca tcgtcgcagc tccaccaatg aaccacgtct
    26701 gatggtcagt accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa
    26761 ccattttggc attgcggtgg tgccgttgat ttccagttat cagccttcca ccgtggcggt
    26821 gaacatgaat gacttacccg acggcgtaac agtggcagaa aacgttatca aagaaacgtg
    26881 gattgaaggc gcgataggtt acaaatcgct ggcttcccgt tccggtaaag acgttaacgt
    26941 catcattcgc aacgccagcg gtcagttccc tcccctcggt gcggatatcc gccaggataa
    27001 cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc
    27061 tgaaaatcaa caatttaccg tgatctgggg tgatagccag cgttgctcgc tccatcttcc
    27121 tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg
    27181 aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac
    27241 gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt
    27301 ctgcgcgtgg ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat
    27361 gaaaaagggg agaaaagcga tgatcttctg gttgccctgc caccgataca acgtctggaa
    27421 ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc
    27481 gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctccctcccc agaaaaaaac
    27541 agcggccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtatt ctggcgaccg
    27601 gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag
    27661 ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctatctgggg
    27721 agaaatgaaa aaggcgttct tcccggcttc aaaaccgtga tggttgcgcc ttttagtaca
    27781 gtaattacaa acaccggaag ttatagcggc agtcagtttt atctcggtta tatggatgat
    27841 tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccgttt acaagcagtg
    27901 gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg gtatttctgc
    27961 cattgaagtc tgcgctagcg ctgaactgct attttggtac ttccggcgga acggtagaaa
    28021 aatcggaagc gattcaaccc tttgctgtgc caggtaatgc caaacccggc gataagatct
    28081 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg
    28141 aaagcgaaca tgtctacgca tgggtaaacc cctacccggg ggtacaggat cgttattatc
    28201 aactcggcgt gacatacaat ggtgtcgatt acgatgccag tctggggaaa agccgcatcg
    28261 acaccaacca gtgtatcgac agtaagaaca ttaacattta tacccctgag cagattatcg
    28321 cgatgggctg gcagaacaaa atatgctcgg gtgaccccgc taatattcac atgtcgcgaa
    28381 cctttcttgc tcgtatgcgg ttatacgtta agattcgcga aataccgccg catgattatc
    28441 aaagttcgct tagcgactac atcgtcgtgc aatttgatgg tgccggtagc gttaacgaag
    28501 accctaccgc ccaaaacctg aaataccata ttactggtct ggaaaacatt cgtgtactgg
    28561 attgcagcgt caatttttct atttcaccag aaacacaggt gattgatttt ggtaaattta
    28621 acctgctgga tattcgccgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat
    28681 cacaaaatga ccagtgcacc gacggattta aggtcagctc ttcgttttat accgaagaaa
    28741 cattgattga agaggataaa gcactgctga ttggtaatgg actgaagcta cgtttgctgg
    28801 atgaaaattc ttcaccctat actttcaaca aatacagcga atatgctgat ttcaccagcg
    28861 atatgctgat ctacgaaaaa acctatacgg ctgaactttc gtccattccc ggcaccccca
    28921 tcgaagcggg tcccttcgat acagtggtgc tttttaagat taactataac taatttatca
    28981 tgatgccctt tgcagttagt aaagggcctc tctttcagaa aattgctgat tttccccttt
    29041 tccttgattg ctgttatttt ttcctggccc ggctttcttt atgctgtatc gatacaccaa
    29101 agaaaaaata atgagggaac gaaggatgcc agttttgcag tggggaatgt tatgtgtgct
    29161 ctcacttctc ctttcaattg gtttcctcgc ggttcatctc ccggcggcgc tattacttgg
    29221 gccgatgatc gccgggatca tctttagtat gcgcggaatt actctgcaac ttccccgctc
    29281 cgcttttctc gccgcccagg ccattcttgg ctgcatgatt gcgcaaaacc tgaccggttc
    29341 gatcctgacc acgctagcgg tgaactggcc gattgtgtta gctattttac tggtgacgtt
    29401 actttccagc gccatcgtgg gttggttatt ggtgcgctat agctcactgc ctggaaatac
    29461 cggtgcctgg ggttcttctc ctggcggcgc ggcggcaatg gtcgctatgg cgcaagatta
    29521 cggcgcagat attcgcctgg tggcgtttat gcaatatctg cgagtgctgt tcgtcgcagg
    29581 ggccgctgtt ctggtcaccc gcatgatgct gggcgataac gctgaagcgg ttaaccagca
    29641 gatcgtctgg tttccgccag tgagcattaa tctcctgctt acgattttgc tcgcggtcgt
    29701 tgccggtaca gtgggatgct tgctacgtct tccttccggc acgatgctca tcccgatgct
    29761 ggcgggcgca gtgctccagt ctggtcagct catcaccatc gaactaccgg aatggctgct
    29821 tgcgatggcg tatatggcaa ttggttggcg gattggtctt ggtttcgata aacaaatctt
    29881 actgcgggca ttgcgcccgc taccgcaaat cctgctgtcg atttttgctc tgctggctat
    29941 ttgtgcgggt atggcgtggg ggctgacccg gtttatgcat attgatttta tgaccgccta
    30001 cctcgccacc aaccccggcg ggcttgatac cgtggcggtc atcgccgcag ggagcaatgc
    30061 cgatatggcg ctcatcatgg cgatgcaaac cctgcgcctg ttcagtattt tgctgacggg
    30121 acctgccatt gcgcggttta tttcaaccta tgcgccgaag cgttctgcct aatgctggca
    30181 gccagggcac cagtaaaacg ggcgagatga cagcgtggtt ttctcaatga tgccgccaca
    30241 gcgttcgcac agttcgccat cacgatgaaa aaccttaaag cgaaacagcg ccccatgatg
    30301 cttattctca tccacctgcc cccgcgtagc gtaggaaaat cgaggaatat ccagtaacgc
    30361 gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc gctttatgat ttccagtcaa
    30421 cccaacctgc cagaggatct ccacccgcaa ataattgcca agcccagcca gaaacgcctg
    30481 atcgagcagt aatccagcaa actgacggtt acgaaagcgc ggcgacaata accgttcctt
    30541 taccacctcc ggcgtcagat tcggatccag cacatcgggg ccgactcgtt gtaaaaacgg
    30601 atgcgtgatc agttgttccg gcgtcaacat ctcaatatcc gaggcgctat aaagcagaat
    30661 agttttgtca accgtttgca gttttacccg caatactcgc atagtctgcg gctcttcgcc
    30721 ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg ctgtagagcg ttaagtcgtt
    30781 ggaaaaatga gttaacaacg ccttaccacg cgtttccaca tgggtaacgt gttgaccgat
    30841 aagttgtgat tgataaggtt ttaactgcgg gaaggcaaac cagacatcag ttagtggttt
    30901 gcctttgatc gccgcctcca ggttatccgc tgcacggcgg atctccgggc cttcaggcat
    30961 ggtgatatcc ttttttcatt ctttattaaa cggggttatg ctgcgacaac aatccccttt
    31021 tcggcaaatg tagagcgtag tcggcgggcg aatgccagtg cgtgctcgcc gtcgccatgc
    31081 aggcagaccg tttgcgccgt gaccgttgcc cattcgccgg tgatgctttt gactctgccg
    31141 tgttgcacca tctccagcgt ttgcgccagc gcctgttctt cgttttcaat caacgcgcct
    31201 ggctggcttc gcggcaccag cgaaccgtct gcctgataac cgcgatcggc aaacacttcc
    31261 tcgcgcgttg tcaggccata ttgcttgcct gcacgaatca gctcgcttcc tgccagcccg
    31321 acgagaatca atgccggatc gcaagcgtat accgctctgg cgatggcgtc tgccagttgt
    31381 gcctctttcg ctgcctggtt gtataacatg ccgtgaggtt tgacatgacg cataacaccg
    31441 ccttgcgcac gggcaatcgt tgccagcgcg ccaatttgat acagcgtctg ggcgtaaacg
    31501 gtttctggcg gcagctgcat gacgctgcgc ccaaaatttt ccctgtcggg aaaactcggg
    31561 tgagcgccaa tcgcaacacc attttttatt gcttcacgca cgcaagcctg catggtttgc
    31621 gcatcgcctg catgaaatcc acaggcaata ttggcagagg aaaccagcgt taatagctct
    31681 gcgtcgctgg catagccttc gcccagatcg gcgttcaggt caattttcat tgtgcagccg
    31741 ccacgctaat tgttcgaaat aacgttgctg atcttgccgt gctttcagtg cttcttccag
    31801 tgaacactgg acaaaatgaa tcggctgacc gagcggaatt tgcgccagat ggtacatatc
    31861 agcctcaatg atacaggcaa tacgcggata accaccggtg gtctgtgcgt cgttcatcaa
    31921 cacaatcggc tgcccgttat gcggcacctg caccacgccc ggtaacaaac catgagataa
    31981 cagttcgcga tcggtggtac gttttaaaat ttgcccctgt aagcgatagc ccattcggtt
    32041 actttgcgag ctaagctgcc agggcgaacg ccagaatgca tcctgcgagg cgcgatcgaa
    32101 ctcatgatat tccggccccg gcaaggcgcg aatgcggttg ccccacagca gctgtttaac
    32161 gccctgcgct tccataaaat cacgcttcga tttgccaatc gggagtcggt caccatcctt
    32221 cagtaaacgg ccttccagcc cgccaatccc cactttgaga tcggtgctgc atgaccccat
    32281 taccggcgga acatcaatac cacccgcgac cgccagataa ctgcgcatcc cgtgctgcgg
    32341 gcgtttaagc gttaaacgct gacctgcttt catcggcaag cgccagccgg tccagacggc
    32401 gttatcatcc agccgcgctt cgcaaccggc ccccgtcaga gcaaaccacc catcagtttc
    32461 gaactcaacc gttaactgac cgagcgtgat ctccagcgcg ggggcattgg cgtcattacc
    32521 caccagtagg ttagcaatgc gcagcgcagg catatccagt gcgccgcagt ggctgatacc
    32581 cgactggcga aaaccgtgac gaccgccatc ctgcacagtg gtatacatac ccgcacgaat
    32641 aatcttcagc atactccctc cttctgcggt acaaagcgca cgctgtctcc cggacgtaat
    32701 aagatgggtt cgtcacgcgc cggatcaaac aggctgagtg aggtatgacc aatcaactgc
    32761 cagccacccg gcgttgccaa cggataaata ccagtctgcg gcccgccgat cccgacagaa
    32821 cctgccggaa cgagcaagcg cggttcagcg cgccgtggcg tgtgtagttg ttccggcaaa
    32881 ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc agaccacgta ttccacggag
    32941 gagtgcaatt caacaacctg tttttcgctc aatccacaat gcgccgcgac caccgccaaa
    33001 tccggtccgg ctgcgccacc gtaaaccacc ggaatttcaa taaagcgaga ctccggctcc
    33061 agcgcctcac tctcctccca ccagcgttgc aaacgctcta tggcatccag cgccagcgac
    33121 tcaggattac gcaaaatcac tgtgatattg ttcatgccag gaatggcttc aaccacattc
    33181 ggcatatcca ccagacgctg tgccagtcgc cagatccgtt tctggctagc cagcgtcacc
    33241 ggcggttcca gctccagcac taccgccgtt tcacctatca gataacaacg cgctcgttgc
    33301 actttcgatc cctctgatta ttcgttatgc aggattagga atatcaataa aggtcacatc
    33361 aagatcggta ttttcattca gccactcgct caatgcgcga ataccaccgc gttcagtggc
    33421 atggtgaccc gcagcataaa aatgcaatcc ctgctcgcgg gctgaatgaa tggtctgttc
    33481 tgaaacttcg ccagtaataa aagcatccac gccaaaacgc gcggcgctat cgataaaact
    33541 ctgcccgccg cccgtgcacc aggcgacgcg ctgaaccacc tcaggtccgg tatcgccaca
    33601 ccataacggc ttacgtccca gacgcgcttc aatccaggaa gccagctcca gtcccggcac
    33661 aggcatggtc agttcgcccc acggcaccaa cggctcaatt tcgcccatga ccgtgatccc
    33721 cagtaacgcc gccagttgtg cgttattacc cagctccgga tgcgcgtcca ggggcagatg
    33781 ccagccatac aggttgatat cattcgccag caacgttttt aaacggttac gcttcattcc
    33841 gcgaatgacc ggagattcac ctttccagaa gtagccatga tgcacaatga ccgcatccgc
    33901 gcccagacgc accgcttcat cgagcaaagc ctggctggcg gtgacaccgg taacaatttt
    33961 ttgcaccgtc tctttgcctt ccacctgcaa accattcggc gcgtaatcac taatcgccgc
    34021 gctgttcagt ttttcgttaa tcagttgttc cagttcggtg tttttcatct tcgcgccttc
    34081 cgtattctga tagctacacc gttaacatag cgttacctcg cctgttcgtc gatagccaat
    34141 cttttgacat attaagaaac aaacccgacg ccaaaagacg caaattgttt atatcatcac
    34201 gcttcagcta atgtatattt atgcaatcag acagcatatt gcataaataa tcagatctgt
    34261 atcaaagttt taactttaag gaaacacaag cacgccgcgc aactattacg tgacgttgca
    34321 gtcaattgtg gaattattat gaataaacac gcatcacaac cgcgcgctat ttactatgtc
    34381 gttgcgctgc aaatctggga atattttagc ttttacggca tgcgtgccct gctgatcctc
    34441 tatctcacca atcaactaaa atatagcgat aatcacgcct acgagttatt tagcgcctac
    34501 tgttcgctgg tatatgtcac gccaatcctc ggtggctttt tggcggataa agttctcggc
    34561 aatcgcatgg cggtgatgct gggtgcgttg ttgatggcga tcggtcatgt ggtgctgggt
    34621 gccagtgaga tccatccgtc attcctctat ctgtccctgg cgattatcgt ttgtggctat
    34681 ggtctgttta aatccaacgt cagttgcctg ctcggcgagc tgtatgagcc aaccgatccg
    34741 cgtcgtgatg gcggtttctc gctgatgtat gcggcgggta acgtggggtc tattatcgca
    34801 cctatcgcct gtggctacgc ccaggaagag tacagttggg cgatgggctt tggcctggcg
    34861 gcggttggca tgatcgcggg tctggtcatt ttcttatgtg gcaatcgtca tttctctcat
    34921 acccgcggcg ttaacaaaaa agtactgcgt gcgacaaact ttctcctgcc caactgggga
    34981 tggctgctgg ttctgctggt ggcaacacct gcgctgatca ccgtactgtt ctggaaagag
    35041 tggtcggtat acgccttgat tgtcgcgact attattggcc tgggtgtatt ggcaaaaatt
    35101 tatcgcaaag cagaaaacca gaaacagcgt aaggagctgg ggctgattgt taccctcacc
    35161 ttcttcagca tgttgttctg ggcattcgca caacaggggg gtagctcgat tagcctttat
    35221 atcgaccgct tcgttaaccg cgatatattt ggttataccg ttccgactgc tatgttccag
    35281 tcgattaatg ccttcgcagt tatgctgtgc ggtgtgttcc tggcgtgggt ggttaaagaa
    35341 agcgtcgcag gtaatcgtac cgtgcgcatc tgggggaaat ttgctcttgg tctcggcctg
    35401 atgagcgccg gattctgcat tctgacctta agcgcccgct ggtctgcaat gtatggtcac
    35461 tcttctctgc cactgatggt gttaggcctg gcggtgatgg ggtttgcgga actgtttatc
    35521 gacccggttg ctatgtcgca gattacgcgc attgaaatcc ccggcgtgac cggcgtatta
    35581 accggcattt atatgctgct ttccggcgcg attgcgaact atctggccgg cgtgattgcc
    35641 gatcagacat cgcaggcttc gtttgatgct tccggagcga tcaactactc catcaatgca
    35701 tatattgaag tatttgatca aattacctgg ggcgcactgg cgtgtgtagg actggtgctg
    35761 atgatttggc tgtatcaggc gcttaagttc agaaaccgcg cgctggcgct ggaatcttaa
    35821 caggccagca ccgatgcctg ataagacgct cgctgcgttt tatcaggctc ctttcctcgc
    35881 ttcttcgtac gctgccagcg ttcgcagcct tgcctctttg tgatcaacta tcggttgcgg
    35941 ataatccagc gtcacacctg ctttctgcgc ccacttccac ggctcatgca ccactttccc
    36001 tggcacatcg cgcagttccg gtagccactg gcggataaac tcgccctcac ggtcaaattt
    36061 ctcgccctgg gttgtcgggt taaaaatacg aaaatacggc gctgcatcgg ttccggttga
    36121 agcggcccac tgccagccac cgttattggc tgccaaatca ccatcaatca gctgcgacat
    36181 gaaatatcgc tcaccttcgc gccagtcgat caataaatct ttcaccaaaa aactggctgt
    36241 aatcatccgt agccggttat gcatccagcc ggtgctgtta agctgacgca tggcagcatc
    36301 aacaatcggg tatcccgttt tgccttcctg ccaggccttt aaatgcgcgg aattgctctg
    36361 ccactgcacg cggtcggtcc agacaataaa cggacgatgt ttacacagcg aggggtaata
    36421 cgttatcaga tgacggtaaa actcgcgcca gatcagctca ttaagccaga tactaccggc
    36481 cccaccgtcc agcgcctgcg gctgttccgc caacaggcga tgcaaacact ggcgaggcga
    36541 taacccgccc gttgccaggc tggcggacaa acggctggtg ccttctaccg cagggaaatc
    36601 tcgctgttgc tcatactctc cggcaccgtt ctggcaaaac tggcgtaatt gcgcaatcgc
    36661 cgctttctct tccaccggga aatgcgcagt atcgaaaggc tgacgaggat aattcagcgt
    36721 aatggatggc gcgggatcta tcgatccgct actacgtact tttggcgcag cgacgcactc
    36781 cggcatccct tctcgcagcc gtttaagcca ggcgttttta aatggcgtaa agactttgta
    36841 catctcatga ttgccagtca tcaccgcccc cggcggcagg atcacgccgt cgtcaaatcc
    36901 ttcacacacc acgttacgca gcgccctctc agcctgcaca tcccgcgccc gctcattcac
    36961 ctcatactga tagttataaa acagatgggt aacacgattt tccgcgcaca cctgtttaac
    37021 tatttcgacg ctggcggcaa agtcatccac ttcatgaaac agcaaaggaa tgcctttttc
    37081 cgccagcgcg atttgtagcc cgttcagttg tgcattgata aattcagcct gacgcggcga
    37141 catattatgc gccgcccact ggcgtggtgt agcgatatac aacgccagca cgcttgcaga
    37201 cgaattgcgg caggcggcag ccagcgcgag attatcatgc tgacgtaaat cctggcgaaa
    37261 ccagaccaga tgggtagtca taaaactcct gaatggcgca agttaatcac acgcggccaa
    37321 agttcgaaat aacgctgccc tgaaagccag gcgtcaggat aaagcgccat atagtgtttg
    37381 atacgcatca gcggcgcaag aagcggttgc tcgccaccgc gataactgtc gataagcgcc
    37441 gccagctgct ggcgttctgt ggaatcaata tgagggcgaa aataaccctg aacgtgcatc
    37501 aaaacattgg tgtggtcacg tcggttggcg gggtgagaaa gcaaaacaac tacgcgttgg
    37561 cggtactggt tgtaatagtc atccagattt tgccactggt gaatatcagc cacgaacggg
    37621 ccaagtttgc ggtactcggg ctgggaatgg gccagaaaga cgagtttata gcggctgtga
    37681 taatcgttga gtgccccgcg cgttaagcca tgttctttca gtcgatgtaa ctcataaagc
    37741 gcgaagatac ggattagagt gctttcatca aatcgttgct gattcattac tgctcctgag
    37801 atattaacag gagtaagtgt agacgttggc aaatgatgtt tgtgaagtta atcctgttgt
    37861 agcagggcaa aattacgaaa gcacgctccc cgcaaggact gacgccagat attttctgtc
    37921 catggctgcc ctttccattc aaacgtgaaa tcaataagtt catcagggac atcgcaaaca
    37981 atatgaagac ggatttcttc tctgccgtga ctcttttcac tcattgcgta actgccatgc
    38041 tctggattat ttaattcttt cagcggaaat ttttcctcga aggctttatt ta
//
LOCUS       DAZGTJ010000041        37992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000041 DAZGTJ010000000
VERSION     DAZGTJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.041"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..213
                     /locus_tag="ACHK8E_002560"
     CDS             <1..213
                     /locus_tag="ACHK8E_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344808.1"
                     /translation="EFQDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCL
                     LLFHPFCCIRTQNGAFLHAKRLAQGQ"
     gene            257..811
                     /gene="yrdA"
                     /locus_tag="ACHK8E_002561"
     CDS             257..811
                     /gene="yrdA"
                     /locus_tag="ACHK8E_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP0344809.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(787..1044)
                     /gene="yrdB"
                     /locus_tag="ACHK8E_002562"
     CDS             complement(787..1044)
                     /gene="yrdB"
                     /locus_tag="ACHK8E_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP0344810.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(1041..1859)
                     /gene="aroE"
                     /locus_tag="ACHK8E_002563"
     CDS             complement(1041..1859)
                     /gene="aroE"
                     /locus_tag="ACHK8E_002563"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP0344811.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            complement(1864..2436)
                     /gene="tsaC"
                     /locus_tag="ACHK8E_002564"
     CDS             complement(1864..2436)
                     /gene="tsaC"
                     /locus_tag="ACHK8E_002564"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP0344812.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            complement(2441..2983)
                     /gene="yrdD"
                     /locus_tag="ACHK8E_002565"
     CDS             complement(2441..2983)
                     /gene="yrdD"
                     /locus_tag="ACHK8E_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0344813.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            complement(3012..3485)
                     /gene="smg"
                     /locus_tag="ACHK8E_002566"
     CDS             complement(3012..3485)
                     /gene="smg"
                     /locus_tag="ACHK8E_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP0344814.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            complement(3457..4581)
                     /gene="dprA"
                     /locus_tag="ACHK8E_002567"
     CDS             complement(3457..4581)
                     /gene="dprA"
                     /locus_tag="ACHK8E_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP0344815.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            4711..5220
                     /gene="def"
                     /locus_tag="ACHK8E_002568"
     CDS             4711..5220
                     /gene="def"
                     /locus_tag="ACHK8E_002568"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP0344816.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            5235..6182
                     /gene="fmt"
                     /locus_tag="ACHK8E_002569"
     CDS             5235..6182
                     /gene="fmt"
                     /locus_tag="ACHK8E_002569"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP0344817.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            6228..7517
                     /gene="rsmB"
                     /locus_tag="ACHK8E_002570"
     CDS             6228..7517
                     /gene="rsmB"
                     /locus_tag="ACHK8E_002570"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP0344818.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            7539..8915
                     /gene="trkA"
                     /locus_tag="ACHK8E_002571"
     CDS             7539..8915
                     /gene="trkA"
                     /locus_tag="ACHK8E_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP0344819.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            9045..9455
                     /gene="mscL"
                     /locus_tag="ACHK8E_002572"
     CDS             9045..9455
                     /gene="mscL"
                     /locus_tag="ACHK8E_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP0344820.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(9452..9670)
                     /gene="arfA"
                     /locus_tag="ACHK8E_002573"
     CDS             complement(9452..9670)
                     /gene="arfA"
                     /locus_tag="ACHK8E_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP0344821.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(9726..10151)
                     /gene="zntR"
                     /locus_tag="ACHK8E_002574"
     CDS             complement(9726..10151)
                     /gene="zntR"
                     /locus_tag="ACHK8E_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP0344822.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(10162..10530)
                     /gene="yhdN"
                     /locus_tag="ACHK8E_002575"
     CDS             complement(10162..10530)
                     /gene="yhdN"
                     /locus_tag="ACHK8E_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP0344823.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLLPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            complement(10637..11020)
                     /gene="rplQ"
                     /locus_tag="ACHK8E_002576"
     CDS             complement(10637..11020)
                     /gene="rplQ"
                     /locus_tag="ACHK8E_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP0344824.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(11061..12050)
                     /gene="rpoA"
                     /locus_tag="ACHK8E_002577"
     CDS             complement(11061..12050)
                     /gene="rpoA"
                     /locus_tag="ACHK8E_002577"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP0344825.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(12076..12696)
                     /gene="rpsD"
                     /locus_tag="ACHK8E_002578"
     CDS             complement(12076..12696)
                     /gene="rpsD"
                     /locus_tag="ACHK8E_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP0344826.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(12730..13119)
                     /gene="rpsK"
                     /locus_tag="ACHK8E_002579"
     CDS             complement(12730..13119)
                     /gene="rpsK"
                     /locus_tag="ACHK8E_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP0344827.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(13136..13492)
                     /gene="rpsM"
                     /locus_tag="ACHK8E_002580"
     CDS             complement(13136..13492)
                     /gene="rpsM"
                     /locus_tag="ACHK8E_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP0344828.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(13639..13755)
                     /gene="rpmJ"
                     /locus_tag="ACHK8E_002581"
     CDS             complement(13639..13755)
                     /gene="rpmJ"
                     /locus_tag="ACHK8E_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP0344829.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(13787..15118)
                     /gene="secY"
                     /locus_tag="ACHK8E_002582"
     CDS             complement(13787..15118)
                     /gene="secY"
                     /locus_tag="ACHK8E_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP0344830.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(15126..15560)
                     /gene="rplO"
                     /locus_tag="ACHK8E_002583"
     CDS             complement(15126..15560)
                     /gene="rplO"
                     /locus_tag="ACHK8E_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP0344831.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(15564..15743)
                     /gene="rpmD"
                     /locus_tag="ACHK8E_002584"
     CDS             complement(15564..15743)
                     /gene="rpmD"
                     /locus_tag="ACHK8E_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP0344832.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(15747..16250)
                     /gene="rpsE"
                     /locus_tag="ACHK8E_002585"
     CDS             complement(15747..16250)
                     /gene="rpsE"
                     /locus_tag="ACHK8E_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP0344833.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(16265..16618)
                     /gene="rplR"
                     /locus_tag="ACHK8E_002586"
     CDS             complement(16265..16618)
                     /gene="rplR"
                     /locus_tag="ACHK8E_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP0344834.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(16628..17161)
                     /gene="rplF"
                     /locus_tag="ACHK8E_002587"
     CDS             complement(16628..17161)
                     /gene="rplF"
                     /locus_tag="ACHK8E_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP0344835.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(17174..17566)
                     /gene="rpsH"
                     /locus_tag="ACHK8E_002588"
     CDS             complement(17174..17566)
                     /gene="rpsH"
                     /locus_tag="ACHK8E_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP0344836.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(17600..17905)
                     /gene="rpsN"
                     /locus_tag="ACHK8E_002589"
     CDS             complement(17600..17905)
                     /gene="rpsN"
                     /locus_tag="ACHK8E_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP0344837.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(17920..18459)
                     /gene="rplE"
                     /locus_tag="ACHK8E_002590"
     CDS             complement(17920..18459)
                     /gene="rplE"
                     /locus_tag="ACHK8E_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP0344838.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(18474..18788)
                     /gene="rplX"
                     /locus_tag="ACHK8E_002591"
     CDS             complement(18474..18788)
                     /gene="rplX"
                     /locus_tag="ACHK8E_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP0344839.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(18799..19170)
                     /gene="rplN"
                     /locus_tag="ACHK8E_002592"
     CDS             complement(18799..19170)
                     /gene="rplN"
                     /locus_tag="ACHK8E_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP0344840.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(19335..19589)
                     /gene="rpsQ"
                     /locus_tag="ACHK8E_002593"
     CDS             complement(19335..19589)
                     /gene="rpsQ"
                     /locus_tag="ACHK8E_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP0344841.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(19589..19780)
                     /gene="rpmC"
                     /locus_tag="ACHK8E_002594"
     CDS             complement(19589..19780)
                     /gene="rpmC"
                     /locus_tag="ACHK8E_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP0344842.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(19780..20190)
                     /gene="rplP"
                     /locus_tag="ACHK8E_002595"
     CDS             complement(19780..20190)
                     /gene="rplP"
                     /locus_tag="ACHK8E_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP0344843.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(20203..20904)
                     /gene="rpsC"
                     /locus_tag="ACHK8E_002596"
     CDS             complement(20203..20904)
                     /gene="rpsC"
                     /locus_tag="ACHK8E_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP0344844.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(20922..21254)
                     /gene="rplV"
                     /locus_tag="ACHK8E_002597"
     CDS             complement(20922..21254)
                     /gene="rplV"
                     /locus_tag="ACHK8E_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP0344845.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21269..21547)
                     /gene="rpsS"
                     /locus_tag="ACHK8E_002598"
     CDS             complement(21269..21547)
                     /gene="rpsS"
                     /locus_tag="ACHK8E_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP0344846.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(21564..22385)
                     /gene="rplB"
                     /locus_tag="ACHK8E_002599"
     CDS             complement(21564..22385)
                     /gene="rplB"
                     /locus_tag="ACHK8E_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP0344847.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(22403..22705)
                     /gene="rplW"
                     /locus_tag="ACHK8E_002600"
     CDS             complement(22403..22705)
                     /gene="rplW"
                     /locus_tag="ACHK8E_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP0344848.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(22702..23307)
                     /gene="rplD"
                     /locus_tag="ACHK8E_002601"
     CDS             complement(22702..23307)
                     /gene="rplD"
                     /locus_tag="ACHK8E_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP0344849.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(23318..23947)
                     /gene="rplC"
                     /locus_tag="ACHK8E_002602"
     CDS             complement(23318..23947)
                     /gene="rplC"
                     /locus_tag="ACHK8E_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP0344850.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(23980..24291)
                     /gene="rpsJ"
                     /locus_tag="ACHK8E_002603"
     CDS             complement(23980..24291)
                     /gene="rpsJ"
                     /locus_tag="ACHK8E_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP0344851.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(24529..24948)
                     /gene="gspB"
                     /locus_tag="ACHK8E_002604"
     CDS             complement(24529..24948)
                     /gene="gspB"
                     /locus_tag="ACHK8E_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFP0344852.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            complement(24950..26419)
                     /gene="gspA"
                     /locus_tag="ACHK8E_002605"
     CDS             complement(24950..26419)
                     /gene="gspA"
                     /locus_tag="ACHK8E_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFP0344853.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            26599..27414
                     /gene="gspC"
                     /locus_tag="ACHK8E_002606"
     CDS             26599..27414
                     /gene="gspC"
                     /locus_tag="ACHK8E_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP0344854.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRHERTQ"
     gene            27398..29350
                     /gene="gspD"
                     /locus_tag="ACHK8E_002607"
     CDS             27398..29350
                     /gene="gspD"
                     /locus_tag="ACHK8E_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP0344855.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            29360..30841
                     /gene="gspE"
                     /locus_tag="ACHK8E_002608"
     CDS             29360..30841
                     /gene="gspE"
                     /locus_tag="ACHK8E_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP0344856.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            30838..32034
                     /gene="gspF"
                     /locus_tag="ACHK8E_002609"
     CDS             30838..32034
                     /gene="gspF"
                     /locus_tag="ACHK8E_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP0344857.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            32044..32481
                     /gene="gspG"
                     /locus_tag="ACHK8E_002610"
     CDS             32044..32481
                     /gene="gspG"
                     /locus_tag="ACHK8E_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP0344858.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            32489..32998
                     /gene="gspH"
                     /locus_tag="ACHK8E_002611"
     CDS             32489..32998
                     /gene="gspH"
                     /locus_tag="ACHK8E_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP0344859.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            32995..33372
                     /gene="gspI"
                     /locus_tag="ACHK8E_002612"
     CDS             32995..33372
                     /gene="gspI"
                     /locus_tag="ACHK8E_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP0344860.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            33365..33952
                     /gene="gspJ"
                     /locus_tag="ACHK8E_002613"
     CDS             33365..33952
                     /gene="gspJ"
                     /locus_tag="ACHK8E_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP0344861.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            33945..34928
                     /gene="gspK"
                     /locus_tag="ACHK8E_002614"
     CDS             33945..34928
                     /gene="gspK"
                     /locus_tag="ACHK8E_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP0344862.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            34943..36106
                     /gene="gspL"
                     /locus_tag="ACHK8E_002615"
     CDS             34943..36106
                     /gene="gspL"
                     /locus_tag="ACHK8E_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP0344863.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            36103..36564
                     /gene="gspM"
                     /locus_tag="ACHK8E_002616"
     CDS             36103..36564
                     /gene="gspM"
                     /locus_tag="ACHK8E_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP0344864.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            36564..37241
                     /gene="gspO"
                     /locus_tag="ACHK8E_002617"
     CDS             36564..37241
                     /gene="gspO"
                     /locus_tag="ACHK8E_002617"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFP0344865.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(37270..37746)
                     /gene="bfr"
                     /locus_tag="ACHK8E_002618"
     CDS             complement(37270..37746)
                     /gene="bfr"
                     /locus_tag="ACHK8E_002618"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP0344866.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(37818..>37992)
                     /gene="bfd"
                     /locus_tag="ACHK8E_002619"
     CDS             complement(37818..>37992)
                     /gene="bfd"
                     /locus_tag="ACHK8E_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP0344867.1"
                     /translation="GISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREVME
                     DELMQLPEFKESA"
BASE COUNT         9574 a         9868 c         8995 g         9555 t
ORIGIN      
        1 gaatttcagg attttttctc tttgttgtta cgaccatctt gtgaagtgtt tcacgttgtc
       61 gtctcaacgg aggcgcatta tagggatccc aattttttgc acaagtactt ttttgatctt
      121 tttttctgtt tgttgctttt tcaccctttt tgctgcattc gcacacaaaa cggtgctttt
      181 ttgcatgcta aaagacttgc acaaggccaa taatgcaccc aaagtcatta gtaaatcatt
      241 tattgttgag gtgagtatgt ctgatgtttt acgcccatac cgcgatcttt ttccacaaat
      301 cggccagcgc gtaatgatcg acgatagcag cgtcgtgatt ggtgacgttc gtctggctga
      361 tgatgtgggg atctggccgc tcgttgtgat tcgtggagat gtacattatg tacagatcgg
      421 agcacgcacc aatatccagg atggcagtat gttgcatgtc actcataaat cctcgtacaa
      481 cccagatggc aacccattaa ccattggcga agatgtcact gttggtcaca aggtgatgct
      541 ccacggctgc accattggca atcgagtttt ggttgggatg ggctcaattt tacttgatgg
      601 cgcaatagta gaagatgatg tgatgattgg tgcgggtagc ctggtcccac aaaataaacg
      661 gctggagagc ggatatctgt atctcggtag ccccgtcaaa cagatccgcc cgttaagtga
      721 tgaagagaag gctgggttac gctattccgc gaagaattac gtgaaatgga aggacgaata
      781 tctggatcag ggtaaccaga cccaaccttg atcatcttca ctttgttcct gaattaagtt
      841 ttccgcttct tcttccaggt cccagcgatg ctgacgaaaa ctcgctaacc actgttctgg
      901 cgtatctcca gtaaagcgat acgccagact ctcgccagag atcgcacatg tcagttgcat
      961 accattcacg agagcgggaa aacatacaca tcttttattc tcgtcccact cttccctatc
     1021 cggaaactgg atggcctgat tcacgctgac aattcctgtt gcaatagttt gatgaccggt
     1081 tctacatcag gcagaacgcc atgccaaaga aggaaggcat gagccgcctg tgccaccagc
     1141 attcctaaac catcagcatt acgctttgag cctcgctgct cacaccatgc cagaaaagga
     1201 gtttttcctt tctgatagaa catgtcatag caataaatgc ctggatgaat aagcgatgac
     1261 gggatcgccg gaatatcacc actgatgcca ctggatgttg cattaataat gagatcaaac
     1321 tcatgacctt ccagttcgtc catacccaac gcctgaatac tgccagtgtg cgcaaacaat
     1381 ttagccaact cttccgcgcg ggataccgtc cgattagtga ttgtcaccgc acagtccagg
     1441 gaaagaagtg gcagtagtac gccgcgagaa gctccaccag cgccgatgag cagaatacgt
     1501 aaaccaggac ggataaaaga cagacgttcc agatcgctta acaaacctat accatcggta
     1561 ttgtcaccca gcaggcgtcc atcttctaac cgcttgaggg tattaacagc accagccaac
     1621 gctgcccgtt cagtaagctc atccgctctg gcaaaagcct cttctttaaa aggtaccgtc
     1681 acattcgcac ctttaccacc agcactaaag aaagcgttca gtgtgttgat gaaatcattg
     1741 atgggtgcca acacgcgccc atagggatgt tcaatattca gttgctgagc aaattgctga
     1801 tgaatgaatg gcgatttgct gtgggctatc ggattaccaa aaacagcata ggtttccatt
     1861 atgttacccc tgtcgaaaca gttcacccgt cagggcatcg cggatttctg aaggatttaa
     1921 acgccccccc gtttcaccag gcacaaccgg gaacgccgcg ccaaattgtg cgcgaacttc
     1981 gtctactgtt cgacaaggtg gcaatccact caagttggca ctggtagaaa ccagcggttt
     2041 accataagcc tggcacaaag caaccaccaa cggatggtcg gtgactcgta cagcaagcga
     2101 atcaaagcgg cccgtcaacc agcgcggtgt tgtcgcaggc gcgggaaaga caaaggtgac
     2161 aggacctggc cagcgggaaa aaatggtttc acgctgcgca tcagtcagca tggtgtcatc
     2221 aatatagggt ttaagctgct cgtaatttgc cgcgattaaa atcagcccct tatcaaccgg
     2281 acgctgtttt aactccaaca gtcgcatcac tgctgtttcg ctatcaggat cgcacccgac
     2341 accgaaaacg gcttccgttg gataggcgat gacacgtttt tcattgagga catctatcgc
     2401 agctgcgata gcgtctcctt gcaggttatt attcacgtta ttattccgcc gaaaccggct
     2461 ttccacattg tttactggca caaaagtgtt ttacaccctg cgcggttttc ttttcgatga
     2521 gtagcggata atgacactca gggcattctc cagctatggg tttgaagtta atggcaaatt
     2581 gacactccgg gtagcgatca caggagtgaa atgttttgcc ataacgggaa cggcgctgga
     2641 ccagatggcc cgtccgacat tggggacagg taattgctgt ttcgtccggt ttatcgataa
     2701 gttcggtatg ttcgcattca gggtagttac tgcaaccaat aaacatacca aagcgtccct
     2761 ggcgtaatac cagatttgcg ccacatgcag ggcaaacctg cccctccaga actttgacaa
     2821 tatgtccatc cgctgaagat ttcagaggac ggacgtagtc acacgccgga tactgtgagc
     2881 atccaagaaa cggaccgtgt ttcccggatc gaataaccag ttcagccccg cactttgggc
     2941 aggactcatt attacgcacc gtgaacagtg ctgatttcgc cataacaact tatgctgaat
     3001 taaagaaatg attaatgcag cataccttca ttcacttcaa agagtaattc ttccatttgc
     3061 tggtacgcat tttcgcagcc cggaatattg aacaacacca tcaggattac ccatttcagg
     3121 tcttccaggt cgaactctgc ggtatccagc gcgagcactc gctctatcac catttcacgg
     3181 gtttcaaggt taagcacctg aatttgctca aggaaaagca gaaaaccacg acagctggca
     3241 tccagccttt cacactcttc aggggtataa atacgcatgg agagtggatc agaggccagt
     3301 tgcatcggtt ctgccagtcc ttcctgataa tccgcaagct tttccagcca aagcagggca
     3361 ttgtagatat cttctcgatc aaatcctgcg tcggtaagat cctgttcaag tttgtcttga
     3421 tccacacgca actcagcttc tgtgtgaata taggtttcaa acaaatacat tagtacgtcg
     3481 aacatggcat gccctcctca atcggacata gccgccgggt acagctgcga tccatcctgc
     3541 taactccagt tcgagtagtt gagttactac ctctggcaca ggttggccgg cacgttcagc
     3601 gacgacgtca acaggtgtta cctcatctcc tacgttagcc aggagctcag gaaatggcaa
     3661 tgccacgtct tcctgatctg gtgaataaaa tgaattttca ggggcgtctg gcaaccagtg
     3721 caatccaaat tgcaagtttt ccagaatttc ttccggttcc gtcacaagaa tcgcaccttg
     3781 ttttattaac cagtgaggcc cttcgcttcc cggattccct aatggacccg gcaaggcaaa
     3841 aacttcacgc ccctgctcaa gcgcacaacg tgctgtcacc agcgaaccac tgcgcaaagc
     3901 cgcttccacc accagtacac ctttacttag accactgata atgcgatttc ttcgtgggaa
     3961 attgtaagca aggggtggaa catcgagggg aaattccgag acaagagccc caccatgttc
     4021 aagcaaactg gtagccagtc gggcatggcg acgcggatga atggtattaa gtccattccc
     4081 caatacagca atgctgacgc catttacctg taaggccgct ttatgcgcca caccatcgat
     4141 tccacgcgcc agtccactcg taattgtcac tccacgcgtc gccagagttt cgcaaaataa
     4201 tcgtccccat cgctcgccat accatgaatg cgcccgactc cccactacgg caagctgaaa
     4261 tgaatgcagc gcgtgcagtt ctccttcaac aaacagtgcg ccggggtaat ctgtcgtcgc
     4321 aagaagttga ggaggataaa attcgctgtc cgcaggaatt aaatgatggt tgggttgctc
     4381 caaccaagaa agtgagcttt cgatactctt tcgcggaaat gaaagaaagc gttgtgcctg
     4441 ccgcaatgta agcccagttt gctgcaatac aaccgcatca atatgcgact gttttgccag
     4501 ccagtgagct atacggacca tatcatcgcc gtacaagctg ctgatgctca ttaaacgcag
     4561 ccaaatttct gtatcgacca tccttatctc cctgccataa gcagccttag caatctttgc
     4621 gattggtcag tgatgctgtc aatcagaggg ggatttgtct agaatagaag aaataatctt
     4681 tctaactcct gaacacatct ctggagattt atgtcagttt tgcaagtgtt acatattccg
     4741 gacgagcggc ttcgcaaagt tgctaaaccg gtagaagaag tgaatgcaga aattcagcgt
     4801 atcgtcgatg atatgttcga gacgatgtac gcagaagaag gtattggcct ggcggcaacc
     4861 caggtcgata tccatcaacg tatcattgtt attgatgttt cggaaaaccg tgacgaacgg
     4921 ctggtgttaa tcaatccgga gcttttagaa aaaagcggcg aaacaggcat tgaagaagga
     4981 tgcctgtcga tccctgaaca acgtgcttta gtgccacgcg cagagaaagt taaaattcgc
     5041 gccctggacc gcgacggtaa accatttgaa ctggaagcag agggtctgtt agccatctgt
     5101 attcagcatg agatggatca cctggtcggc aaactgttta tggattatct gtcaccgctg
     5161 aaacaacaac gtattcgtca gaaagttgaa aaactggatc gtctgaaagc ccgggcttaa
     5221 ggataagaac taacgtgtca gaatcactac gtattatttt tgcgggtaca cctgactttg
     5281 cagcgcgtca tctcgacgcg ctgttgtctt ctggtcataa cgtcgttggc gtgttcaccc
     5341 agccagaccg accggcagga cgcggtaaaa aactgatgcc cagcccggtt aaagttctgg
     5401 ctgaggaaaa aggtctgccc gtttttcaac cggtttccct gcgtccacaa gaaaaccagc
     5461 aactggtcgc cgatctacag gctgatgtta tggttgtcgt cgcctatggc ttaattctgc
     5521 ctaaagcagt gctggagatg ccgcgtcttg gctgtatcaa cgttcatggt tcactgctgc
     5581 cacgctggcg cggtgctgca ccaatccaac gctcactatg ggcgggtgat gcagaaactg
     5641 gtgtgaccat tatgcaaatg gatgttggtt tagacaccgg agacatgctc tataagctct
     5701 cctgcccgat tactgcagaa gataccagtg gtacgctgta cgacaagctg gcagagcttg
     5761 gcccacaagg gcttatcacc acgttgaaac aattggcaga cggcacggcg aaaccagaag
     5821 ttcaggacga aactcttgtc acttacgccg agaagttgag taaagaagaa gcgcgtattg
     5881 actggtcact ttcggcagca cagcttgaac gctgcattcg cgctttcaat ccatggccaa
     5941 tgagctggct ggaaattgaa ggacagccgg ttaaagtctg gaaagcatcg gtcattgata
     6001 cggcaaccaa cgctgcacca ggaacgatcc ttgaagccaa caaacaaggc attcaggttg
     6061 cgactggtga tggcatcctg aacctgctct cgttacaacc tgcgggtaag aaagcgatga
     6121 gcgcgcaaga cctcctgaac tctcgtcggg aatggtttgt tccgggcaac cgtctggtct
     6181 gatagtccac tcttctaagc ccggtcttgc cgggcatttt tatacttatg aaaaaacaac
     6241 gtaatttacg tagcatggcg gcccaggccg ttgaacaagt cgtcgagcaa gggcaatcat
     6301 taagcaacat tctgccaccg ctccagcaaa aagtttccga taaagacaaa gcacttcttc
     6361 aagagttgtg ctttggcgta ctgcgtacgc tttcgcagtt aaactggctg attaataagt
     6421 taatggcccg tccgatgacc ggcaaacagc ggaccgtgca ttacctgatt atggttggtt
     6481 tgtatcaact gctttatacc cgcataccac ctcatgctgc gctggctgaa acggttgaag
     6541 gcgctatcgc aattaagcgc ccgcaactta aagggttgat aaacggtgta ttacgccagt
     6601 tccagcgtca gcaagaagag ttattagccg agtttaatgc cagtgatgca cgttatctgc
     6661 atccttcctg gttgctgaag cgtctgcaaa aagcgtatcc agagcagtgg caatccatcg
     6721 tcgaagccaa taaccagcgt ccgccaatgt ggctgcgcgt taatcgtacg catcattccc
     6781 gcgacagctg gcttgcattg ctggatgaag caggaatgaa aggtttcccg cctgcggatt
     6841 accctgatgc tgtacgtctg gaaacacctg cacctgttca tgcgctacct ggttttgaag
     6901 acggatgggt taccgttcag gatgcctcag cacaaggttg catgacctgg cttgcgccac
     6961 aaaacggtga acacattttg gatctttgtg ccgcccccgg cggtaaaaca acgcatatcc
     7021 ttgaggtggc accagaagcg caggttgttg cggttgatat tgacgaacag cgcctctctc
     7081 gcgtttacga caatttaaaa cgccttggta tgaaggcaac cgtgaaacaa ggtgatggcc
     7141 gctacccttc ccaatggtgt ggcgagcaac agtttgatcg cattttatta gatgcgcctt
     7201 gttcagcaac cggtgtgatt cgtcgccatc cggatattaa atggttacgt cgcgatcgcg
     7261 atatcccgga actcgcgcaa ttgcagtctg aaattctcga cgccatttgg ccgcatttaa
     7321 aatccggtgg aaccctggtc tatgccacct gttcggtgtt accggaagag aatagcctgc
     7381 agattaaagc ctttttgcaa cgtaccgctg atgccgaact ttgcgaaaca ggaacaccag
     7441 agcaaccggg taaacaaaat ctgcctggtg ccgaagaggg cgacggcttc ttttacgcta
     7501 agctaatcaa aaagtgatga gataacgggt cgcgactgat gaaaattatc attctgggtg
     7561 ccggccaggt tggcggcaca ctggcggaaa acctggttgg cgagaacaac gatattactg
     7621 ttgtcgatac caacggtgag cgtctgcgga ccttgcagga taaatttgac ctgcgagtcg
     7681 tgcaggggca tggctctcat ccacgcgtat tgcgggaggc aggtgccgac gacgccgata
     7741 tgctggttgc tgtaactagt tcagatgaaa ccaatatggt tgcctgccag gtagcctact
     7801 cacttttcaa cacccctaat cgcatcgctc gtatccgctc accagactac gtgcgcgatg
     7861 ccgataaact atttcattca gatgctgtac cgattgatca tctgatcgca ccagagcagt
     7921 tggttatcga taatatttac cgactgattg agtatcccgg cgcattgcag gtggtgaact
     7981 tcgctgaggg taaagtcagc ctggctgtgg ttaaagccta ttatggcggc ccgctgattg
     8041 gtaatgcact ttcgaccatg cgcgaacata tgccccatat cgatactcgt gtggcagcaa
     8101 ttttccgcca cgatcgcccc attcgtccgc aaggttcgac cattgttgaa gctggtgatg
     8161 aagtgttctt tattgccgct tcacagcata tccgcgcggt gatgagtgaa ttacagcgac
     8221 tggaaaaacc gtataagcgg atcatgctgg ttggcggcgg taatatcggt gcagggctgg
     8281 cgcgtcgtct ggaaaaagat tacagcgtta aactcatcga acgtaatcag cagcgcgctg
     8341 ccgaactggc ggaaaagttg cagaatacga tcgtcttttt tggtgatgcg tcggatcaag
     8401 aactgctggc cgaagaacat atcgatcaag ttgatctgtt tattgctgtc accaacgatg
     8461 acgaagccaa tatcatgtcc gccatgcttg ccaaacgtat gggtgcgaaa aaggtgatgg
     8521 tattgatcca gcgtcgcgct tatgtggatc tggttcaggg gagcgtgatc gatattgcga
     8581 tttcaccgca acaagcaact atttctgcgt tgcttagcca tgtgcgaaaa gctgatattg
     8641 ttggtgtttc ctcactgcgc cgcggcgtag cagaagctat tgaagccgtt gctcacggtg
     8701 atgaaagcac ctcacgcgtt gtcggcagag tcattgatga aatcaagcta ccgccaggaa
     8761 cgattattgg agcggtggta cgtggaaacg acgtgatgat tgccaatgac aatctgcgca
     8821 ttgagcaagg cgatcacgta attatgttcc tcacagataa aaagtttatt accgacgtcg
     8881 aaagactctt ccagccaagt cctttcttct tgtaattaat aaggcgtcta atgacgcctt
     8941 attatttccc tttgattatc agggattaat taaattcact cctggcagga aaatagctta
     9001 acatttgtta gacttatgat tgtcggcttc atagggagaa taacatgagc attattaaag
     9061 aatttcgcga atttgcgatg cgcgggaacg tggtggattt ggcggtgggt gtcattatcg
     9121 gtgcggcatt cgggaagatt gtatcttcac tggttgccga tatcattatg ccacctctgg
     9181 ggttattaat tggcgggatc gatttcaaac agtttgctgt cacgctacgc gatgcgcagg
     9241 gggatatccc tgctgttgtg atgcattacg gtgtcttcat tcaaaacgtc tttgattttc
     9301 tgattgtagc ctttgccatc tttatggcga ttaagctaat caacaaactg aatcggaaaa
     9361 aagaagaacc agcagccgcg cctacaccaa ctaaagaaga agtattactg acagaaattc
     9421 gtgatttgct gaaagagcag aataaccgct cttaacaagc gcctgaaagc agaaggccag
     9481 tggtaaaaaa gtgatttact ttcttgccac tggcctccca gttcccccga ttgccatgtt
     9541 ttccttttcg catgtaactg cctttccctt tcttatttct ctctacgcgc tgtcggaata
     9601 agggatcatg tagtaatgct tctatcgcat tatcctttat ctgcccttta gtatgctgat
     9661 atcggctcat aaaaactcca attggttatt taacggcgcg agtgtaatcc tgccagtgta
     9721 aaaaatcaac agccactctt aacgccactc gccccttgtt caagagcttc aagaatcgag
     9781 caataaacac tgctatgggc ggtcccacaa caggcatcgt taaggcgttg gagggaacgc
     9841 tgcatactct gcaactcggc tatccgtgct tcgacttcct gcaatctttc ctgcacaatg
     9901 ccttttgact cctgacaggt atggtgttca ggatcgatac ggatcgacag caactcgcgg
     9961 atcgactcta gactgaaacc tagttgtctg gcatggcgga taaatttcaa tcgctggaga
    10021 tcgctttcgg tatacaggcg aaacccacct tcagtacgca cttcatgctc catcatctgc
    10081 tgtttttcgt aataacgaat cgtgtcgggt gttacttccg ccatttttgc cagctcacca
    10141 atgcgataca tacatactcc actagttatc gttgatttta ttcaacaact tgtcagcata
    10201 atcgccgcgt aaaaaatcgg tgctcagtcc agcttgtcgc agcttcaatt ccagtaatga
    10261 caatcgacgg ctaacctctt gatattgcgg atcatcgtga cggatacctc tcaggatatc
    10321 cagaagctga attgcttccc tccgctgctc aagttctggc agtaagcaac cggcattctt
    10381 cagcaagcga taccccgcac gtaattccgg tggcacatga gaatcatcag ccagtatcaa
    10441 tggttcgccg ctacctgcca ggttatcaaa ctcacctttc gcttgcgctt ctgctatatg
    10501 acgctctgcc cactggtcaa gtaaccacat atatactcca ggggatgaac aaaaagagta
    10561 cagctattgt agataagtgg ggatgctacg ggtataaaaa aacccgccgg ggcgggtttt
    10621 tttacgttgc ttcagattac tctgcagcag cttctgcttt ctctgaacga tcaaccagct
    10681 cgatgtaagc catcggcgcg ttgtcgcctg cacggaagcc acacttcaga atacgagtgt
    10741 aaccaccggc acggctcgcg aaacgcgggc ccagttcgtt aaacagtttt gccacgatct
    10801 cgttatcacg agtacgggcg aatgccagac gacgattagc aacgctatca gtcttggcaa
    10861 gagtaatcag cggctcaact acgcggcgca gctctttcgc tttaggcaga gtcgtcttga
    10921 tgatttcatg acgaaccagt gaacctgcca tattgcggaa catagcctgg cgatggctgc
    10981 tgttgcggtt cagttgacga ccactcttac gatggcgcat gaccttatcc ttctcagtaa
    11041 aaccttaacc tgtgatccgg ttactcgtca gcgatgcttg ccggtggcca gttttccagg
    11101 cgcatgccca gagacagtcc acgggaagcc agcacgtctt taatctcagt aagagatttt
    11161 ttaccaaggt taggcgtttt aaggagctca acctcggtac gctgtaccag atcaccgata
    11221 tagtggatag cttctgcttt aaggcagtta gcagagcgga cagtcaattc cagatcgtca
    11281 acagggcgca gcaggatcgg atcgaactct ggtttctctt ctttcacttc aggctgacgt
    11341 acatcacgta agtcaacgaa agcttccagt tgttcagcca gaatggttgc cgcacgacga
    11401 atcgcctctt caggatcgat tgtgccgttg gtttccattt cgatgaccag cttgtccagg
    11461 tcggtacgct gttctacacg cgctgcttca acattgtagg caatacgctc cacagggctg
    11521 tagcatgcgt cgaccagcag acggccgatt gggcgctcat cttcttccga atgaattcgg
    11581 gtagaagccg gcacataacc acgaccgcgc tgaactttga tacgcatgct aatagacgcg
    11641 ttctcatcgg tcaggtggca gatcacgtgc tgcggcttga cgatttcgac atcaccgtcg
    11701 tgggtgatat cggctgcagt cacagggcca atgccagatt tattcaaggt aagaataact
    11761 tcatctttgc cctgaactct caccgccagc cctttcaggt tgagcaggat ttccaggata
    11821 tcttcctgaa cgccttcttt ggtgctgtac tcatgtagta caccatcaat ctcaacctcg
    11881 gtcaccgcgc aacccggcat cgatgagagc agaatacggc gcagtgcgtt acccagagta
    11941 tggccaaagc cacgctctaa aggctcaagg gtcaccttgg cgtgcgtcga actcacttgc
    12001 tcgatatcaa ccaggcgcgg ttttagaaac tctgtcacag aaccctgcat tgtgtcctct
    12061 ctttggtact aagctttact tggagtaaag ctcgacgatc aggtgttcgt taatgtccgc
    12121 agacagatca gaacgctccg gcttacgctt aaacgtacct tccatcttgc cagcatcaac
    12181 ttccagccag gttggctttt cacgctgctc agccagctcc agagcggctt tcacgcgaga
    12241 ctgcttcttc gctttctcac gaatgcttac aacgtcattc ggactaacct gataagaagc
    12301 gatgttaaca acacgaccgt ttaccataat tgctttatgg ctaaccagct gacgtgcttc
    12361 tgcacgagtg gcaccgaagc ccatacggta tacaacgttg tccagacgac cttccagcag
    12421 agccaacagg ttttcaccgg tgttgccttt cagacgtgct gcttctttgt agtagttacg
    12481 gaactgacgc tccagcacac catagatacg gcgaactttt tgcttttcac gcaactgcac
    12541 accatagtca gacagacgcg gtttacgcgc accgtgctgg ccaggagctt gttcaatttt
    12601 acacttggta tcgatcgcgc gaacgccaga cttaaggaat aagtcggtgc cctcacgacg
    12661 gctcagcttg agcttaggac ccaaatatct tgccattttc tttctccaac aaacctggaa
    12721 aacgaagcgt tatacgcgac gttttttcgg cggacgacaa ccgttatgag ggatcggagt
    12781 cacatcagta atgttagtga tgcggaaacc tgcggcgttc agagcacgaa tagtagattc
    12841 gcggcctgga cccggacctt taaccataac ttccagattc ttgatgccgt attctttcac
    12901 ggcgtcagcg caacgctctg ctgcaacctg agctgcaaac ggagtggatt tgcgagaacc
    12961 acggaaaccg gaaccaccgg ctgttgccca acccaacgcg ttaccctgac gatcagtgat
    13021 agtcacgatg gtgttgttga aagaagcatg gatatgagcc acgccgtcag agacttgttt
    13081 tcttacacgt ttacgtgcac gaattggtgc ctttgccatt attcaatcac cccgattatt
    13141 tcttgatcgg tttgcgcgga cccttacggg tacgtgcgtt ggtcttggta cgctgaccgc
    13201 gaaccgggag accacgacga tgacgcaaac cgcgatagca accaagatcc atcaggcgct
    13261 tgatgctcat gctgatttca cggcgcagat caccttcaac gacaaatttg gcaacttcgt
    13321 cacgcagcgt gtcgatttgt ccttcagaca gctcactgat cttaacatct tcagcgatac
    13381 ccgctgcagc caggatggct ttagaacggg tcttgccgac gccataaatc gaagttaatg
    13441 cgattacggc atgcttatga tcaggaatgt taatgcctgc tatacgggcc actatgcact
    13501 cctactattt aatatgtacg ttccatgctg aaaagcccgt tttcaggata ctcaaatgga
    13561 aacgcacaga catacaaaag attggctggc taatctagcc agctcaaccc aactttgcaa
    13621 gaaaaatatg cgaaaaaatc agccttggcg ctgtttatgc ttcggctcgg cactgcaaat
    13681 cacacggatg acaccatcac gcttaacgat tttgcagtta cggcataatt tcttgacgga
    13741 agcacgaact ttcattttta ctctccgtaa cttctcgggc gaccaattat cggccgtagc
    13801 ctttcaggtt cgccttcttc aatgcagact catactgact ggacatcatc agagtttgca
    13861 cttgagccat aaagtccata atcacgacaa caacgataag cagtgaggtc ccaccaaagt
    13921 agaacggtac tttcattgca tcacgcatga actccgggat caggcagata aaggtaatgt
    13981 acagcgcacc aaccagggtc aggcgggtca ttactttatc gatatacttc gccgtttgct
    14041 ctcccggacg aattcctggt acaaatgcac cggacttctt caggttatct gctgtttcac
    14101 gcgggttgaa aaccaacgcc gtgtagaaga aacagaagaa gatgattgca gacgcataga
    14161 gtaacacata aagcggttgc ccaggctgca aatacagcga aattgttgtc agccagttcc
    14221 aaccagtacc gcccccgaac catgacgcga tggtcgccgg gaacagaata atactggaag
    14281 cgaagattgc cgggattacc cccgccatat tcactttcag cggtaaatgt gtgctctgtg
    14341 cagcatagac acgacgacct tgctgacgtt tcgcgtagtt taccacaatg cggcgttgac
    14401 cacgctcaac aaatacaaca aagaacgtca ctgcaaatac taatactgca accaacagca
    14461 acacgaggaa gtgcaggtcg ccttgacgcg cttgctcgat agtatgggca atggctggcg
    14521 ggagtcccgc gacaataccg gcgaagataa tgattgaaat accgttgccg atacctcgtt
    14581 cagtaatctg ttcgcccaac cacatcagga acatggttcc tgtgaccaga cttacaacag
    14641 cggtgaagta gaatgcaaag cccgggttaa tgaccaggcc ttgcatacca ggcatattcg
    14701 gcagaccggt agcaataccg atcgactgga atattgccag caccagagta ccgtagcggg
    14761 tgtactggct gatcttacga cgaccagact ccccttcttt cttaatttct gccaacgttg
    14821 ggtgaaccac cgtcagaagc tggataatga tcgatgccga aatatatggc atgatcccca
    14881 gagcaaagat agaagcacgg ctgagagcac caccagagaa catgttaaac atttcaatga
    14941 tggtgcctcg ctgttgctca agcagtttgg caagtacagc ggcatcaata ccagggatcg
    15001 gaataaaaga gccaatacgg aacacaatca gcgcaccgat aacaaacagc agtctgcgtt
    15061 tcagctcgcc taagccacct ttggcacttt gaaaatctaa tcccggttgt ttagccatct
    15121 gctacttatt cctcgatttt accgccagca gcttcgatag cagcacgagc gcctttagta
    15181 acacgcaggc cacgaacagt taccggagta gtgacttcgc cagccaggat cactttcgcg
    15241 aactcgatct ggataccgat aatgttagcc gctttcagcg tgttcaggtc tacaacaccg
    15301 ccttctactt tagccaggtc agacagacga acttcggctg taatcgctgc tttacgagaa
    15361 gtgaagccga atttcggcag acgacggtac agaggcatct gaccaccctc gaaaccgcga
    15421 cgtacgccac cgccagaacg agatttctga cctttgtgac cacgaccacc ggttttaccg
    15481 aggccagaac cgataccacg acccaggcgt ttacccgcct ttttggagcc ttcggccgga
    15541 gacagagtat ttaaacgcat ctcttactcc tcaactttaa ccatgaagga aaccgcgttg
    15601 atcataccgc gaatagcagg agtatcctcg cgctctacgg tgtgaccaat acgacgcaga
    15661 cccaggccaa gcagcgttgc cttgtgtttc ggcagacgac cgattgcact gcgggtttga
    15721 gtaattttaa tagtctttgc catggtttat ttccccagaa tttcttcaac ggatttacca
    15781 cgcttggcag cgaccatttc tggggaattc atattttcca ggccatcgat agttgcacga
    15841 accacgttaa tcgggttggt ggaaccatac gctttagcca gaacgttatg aaccccagcg
    15901 acttccagaa cggcgcgcat tgcaccaccg gcgatgatac cggtaccttc ggaagccggc
    15961 tgcatgaata cgcgagaacc cgtatgaaca cctttaacag ggtgttgcag agtaccgtta
    16021 ttcagcgcga cgttaatcat attgcgacgg gctttttcca tcgctttctg gatcgctgct
    16081 ggaacttcac gcgctttacc gtaaccaaaa ccaacgcgac cgttaccatc gccaactaca
    16141 gtcagagctg tgaaggagaa aatacgacca cctttaacgg ttttagatac gcggtttacc
    16201 gcgatcagct tttcctgcag ttcgccagct tgtttttcga tgtgagccat cttacacctc
    16261 taccttagaa ctgaaggcca gcttcacggg cagcatctgc cagtgcctgg acacgaccat
    16321 gatattggaa cccggaacgg tcaaaggata catctttgat gcctttttcc agagcgcgtt
    16381 cagcgacagc tttacccaca gctgcagccg cgtctttgtt accggtgtac ttcagttgtt
    16441 cagcgatagc tttttctaca gtagaagcag ctaccagaac ttcagaaccg ttcggtgcaa
    16501 ttacctgtgc gtaaatgtga cgcggggtac gatgtaccac caggcgagtt gcgcccagct
    16561 cctggagctt gcggcgtgcg cgggtcgcac gacggatacg agcagatttc ttatccatag
    16621 tgttacctta cttcttctta gcctctttgg tacgcacgac ttcgtcggcg taacgaacac
    16681 ccttgccttt gtaaggctca ggacgacggt aggcgcgcag atccgctgca acctggccga
    16741 tcacctgctt atcagcgcct ttcagcacga tttcagtctg agtcggacat tcagcagtga
    16801 tacccgcagg cagctgatgg tcaacaggat gagagaaacc cagagacagg ttaatcacat
    16861 tgcctttaac cgctgcacgg taacctacac caaccagctg cagcttctta gtgaagcctt
    16921 cggtaacacc gataaccatt gagttcagca gggcacgcgc ggtaccagcc tgtgcccaac
    16981 cgtctgcgta accatcacgc ggaccgaagg tcagggtatt atctgcatgt ttaacttcaa
    17041 cagcatcgtt gagagtacga gtcagctcgc cgtttttacc tttgatcgta ataacctgac
    17101 cgttgatttt tacgtcaacg ccggcaggaa caacgaccgg tgctttagca acacgagaca
    17161 ttttttcctc cgattaggct acgtagcaga taatttcgcc accaagacca gcctggcgcg
    17221 ctgcacgatc agtcataaca cctttagagg tagaaacaac tgcgataccc agacccgcca
    17281 taactttcgg cagctcatct ttacgcttat agatgcgcag acctgggcgg ctgacacgct
    17341 gaatgctttc tacaacagct ttgccctgga aatacttcag agtaagttcc agttcaggct
    17401 tggtgtcgcc ttcaacttta aaatcttcaa taaaaccttc ttccttcagc acgttggcga
    17461 ttgccacttt cagcttggag gaaggcatgg tgaccgcagc tttgttcgcg gcctgaccgt
    17521 tacggatacg ggtcagcata tccgcgatcg gatcttgcat gctcatctgt ctttactccc
    17581 gtgattcaat tggtgacaat taccagctag cctttttcag acccgggatt tcaccgcgca
    17641 tagcggcttc acggacctta atacggctca acccgaactt ccgcaggaaa ccatgcggac
    17701 gacctgtttg acggcagcgg ttacgctgac gagacgggct ggaatcacgc ggcagagtct
    17761 gcagcttgag aacagcgttc caacgatctt cgtcggaagc gttcacatca gagatgatcg
    17821 ctttcagttc agcgcgtttc gcgaagtatt tatcagctaa agctacgcgt tttacttcgc
    17881 gtgctttcat tgattgctta gccatttagt aaccctacct tacttgcgga acgggaagtc
    17941 aaaggcagcc agcagagcgc ggccttcttc gtcagatttc gcagtagtgg taatggtaat
    18001 atccaaacca cgaacgcggt cgactttatc gtagtcgatt tctgggaaga tgatctgctc
    18061 acggacaccc atgctgtagt taccacgacc gtcgaaagac ttagcggaca ggccacggaa
    18121 gtcacggata cgaggtacag caatagtgat caggcgctca aagaactccc acatgcgttc
    18181 gccacgcaga gttactttac agccgatcgg atagccctga cggattttga agcctgcaac
    18241 agatttgcgt gctttggtga tcagcggttt ttgaccggag attgctgcca ggtctgctgc
    18301 tgcgttatcc agcagttttt tgtcagcgat cgcttcacca acacccatgt tcagggtgat
    18361 cttctcgacc cgagggactt gcatgacaga attgtagtta aactcagtca tgagtttttt
    18421 aactacttcg tctttgtagt aatcatgcag tttcgccatc gtactactcc aaattacttg
    18481 atagtttcgc tgttagactt gaagaaacgg acttttttac cgtcttcgaa tctaaagcct
    18541 acacggtcag ccttgccggt tgccgcattg aagattgcta cgttggaaac ctgaatagcg
    18601 gcttcttttt caacgatgcc acccggttgg ttcagggccg gaaccggctt ctgatgtttc
    18661 ttaaccaggt tgataccttc aacaatgacc ttgccggaag acaggacatt cttaacttta
    18721 ccgcgtttac ctttatcttt accggttaac acgataactt cgtcatcacg acggattttc
    18781 gctgccatga ttcgctcctt agagtacttc tggtgccaga gagataattt tcatgaactt
    18841 ctcactacga agctcacgag ttaccggccc aaaaatacgc gtaccgatag gctgctcgct
    18901 gttgttgttc agaagaacac aagcattacc atcgaagcga atgacagaac cgtccgggcg
    18961 acgaacaccc ttcttggtgc gcaccactac cgccttcagc acatcacctt ttttgacctt
    19021 accacgcgga attgcttctt tgatggtgat cttgatgatg tcgcctacgc ctgcgtagcg
    19081 acggtgcgag ccacccagaa ccttgataca cattacgcga cgtgcaccgg agttgtcggc
    19141 gacgttcagc atagtctgtt cttggatcat tttagtgctc cgctaatgtc aactactact
    19201 gagacccgaa aatcaggtcg ttaaaaatcc ccatatcgag ggcgcggcat tataacaccg
    19261 cttcaaggat atgggtagaa aaaataaacg gctcatttct gagccgttta ttcgtatcga
    19321 gagagtgtac tgtattacag aaccgctttc tctacaacgc gaaccagcgt ccaggattta
    19381 gtcttggaca gcggacggca ttcgcggatt tcaaccacgt caccgatacc gcattcgttg
    19441 ttctcgtcat gtacgtgcag tttggtcgta cgcttgatga atttaccgta gatcgggtgt
    19501 ttcacaaaac gttcgatagc aacaacaatg gatttctcca ttttgtcgct aacaacgcga
    19561 ccttgcagag tacggatttt atcggtcatt acgcacccgc cttctcgttc agtaaagtct
    19621 taacgcgtgc gacatcgcga cgcacttgct tcaacaggtg agactgttgc agctggccac
    19681 ttgcagcctg catacgcagg ttgaactgct cacgcagcag gttcagcagc tcggtgttca
    19741 gctcttcaac gctcttctca cgcagctctt ttgctttcat tacatcaccg tcttagttac
    19801 aaaggtggtt ttaatcggca gtttcgctgc tgccagcttg aatgcttcac gggccagctc
    19861 ttccggaaca ccgtccattt catacaggac tttacccggc tgaatcaagg caacccaata
    19921 ctccacgtta cctttacctt tacccatacg cactgccagc ggcttttcag tgatcggttt
    19981 gtccgggaac acacggatcc agatcttacc ttgacgctta actgcacggg tcatagcacg
    20041 acgtgctgct tcgatctgac gggcagtcag acgaccacgg ccaacagctt tcagaccgaa
    20101 gctgccgaag ctaacatccg taccctgcgc cagaccgcgg ttacggcctt tgtgcatttt
    20161 acggaatttt gtacgctttg gttgtaacat cagcgacgct ccttatttac ggcctttacg
    20221 ctgctgcttt ttaggctgag cagccggttt ttccggttgt tcaacagcag ccataccacc
    20281 caggatctcg cctttgaaga tccacacttt aacgccgatt acaccgtaag tggtgtgcgc
    20341 ttcagaggtg ttgtagtcga tgtcagcacg cagagtgtgc agcggtacgc gaccttcgcg
    20401 gtaccattcg gtacgtgcga tttctgcgcc gcccagacgg ccgctaactt caactttaat
    20461 acctttagcg cccagacgca ttgcgttctg tacagcacgc ttcatagcac gacggaacat
    20521 aacgcgacgt tccagctgag aagtgatgct gtcagcaacc agttttgcgt ccagttcagg
    20581 cttacgaact tcggcgatgt tgatctgtgc aggaacgcca gcgatgtccg ctacgacctt
    20641 acgcagtttt tctacgtctt cacctttttt accgataacg atacccgggc gagcagtgtg
    20701 aatggttaca cggatgctct tagccggacg ctcgataacg atacgagata cggacgcttt
    20761 agccagttcc ttagtcaggt actgacgtac tttaaaatcg ctgtccaggt tgtcagcgaa
    20821 ttctttggtg ttcgcaaacc aagtagagtt ccatggtttt acaataccca ggcgaatacc
    20881 attaggatgt actttctgac ccattgctag tctccagagt ctcagcgatc ggacacaacc
    20941 acagtgatgt ggctggtgcg cttcaggatg cgatctgcac gaccttttgc acgcggcata
    21001 atgcgcttca tgctcgggcc ttcgtctacg aaaattttcg taactttcag atcgtcaatg
    21061 tcagcgccat cgttgtgttc agcgttagca atggcagatt ccagaacttt cttgaccagt
    21121 acagccgctt tcttgttggt gtaggtcaaa atatccagag cctgcgacac tttcttaccg
    21181 cgaatcaggt cagcaacaag gcgaaccttc tgagcagaag aacgagcatg gcgatgttta
    21241 gcgatagttt ccatctcttc ctcctacctt atttcttctt cgctttttta tcagcagcgt
    21301 ggccgcgata agtacgagtc ggtgcgaatt cacccagttt gtgaccgacc atttcgtcgg
    21361 ttacaaatac cggaacgtgc tgacgaccat tatggacagc gatggtcaaa ccgatcatgt
    21421 taggaaagat cgttgaacga cgggaccaag tgcgcagggg cttcttgtct ccgctttcca
    21481 ccgctttctc taccttcttc agcaagtgca ggtcaataaa aggacctttc ttgagagaac
    21541 gtggcatggc ttatcctcta aaattatttg ctacggcgac gtacgatgaa tttatcagta
    21601 cgcttgttgc tgcgggtctt cttacctttg gtctgaacgc cccacggagt taccgggtgc
    21661 ttaccaaagt tacgaccttc accaccacca tgtgggtggt ctaccgggtt catcgcggta
    21721 ccgcgaacgg tcggacgaac accacgccag cgtgcagcac ctgctttacc cagaacgcgc
    21781 agcatatgct cagcattgcc aacttcgccc agagttgcac ggcagtctgc ttctacttta
    21841 cgcatttcac cagaacgcag acgcagggtg acataagcac catcacgagc aacgatctga
    21901 acgtaagtac cagcggaacg tgccagctga ccgcctttac ctggtttcat ttctacgtta
    21961 tgaacagtag aaccaaccgg gatgttgcgc atcggcaggg tgttacctgg tttgattgca
    22021 gcatcaacgc cagactgaat ctggtcgcca gctttcaggc ctttaggggc caggatgtaa
    22081 cggcgttcac cgtctttgta caaaaccagc gcgatgttcg cggaacggtt cggatcgtac
    22141 tcaagacgtt caacaactgc cgggataccg tctttgttgc gtttgaagtc aacaatacgg
    22201 taagcctgct tgtggccacc accgatatga cgagtggtga tacggccatt gttgttacga
    22261 ccaccggatt tgctgttttt ttccagcaac ggagcaaaag gtttgccctt gtgcagctca
    22321 gggttaacca ctttaacaac gtggcgacga cccggagatg tcggtttaca tttaacaact
    22381 gccattgtat tactcctccg acttactcag cgccgccaac gaagtccaga ttctggcctt
    22441 ctttcagggt gacgtaagct tttttccagt cgctacgacg accgatacgc tgtccgtgac
    22501 gtttaacttt ccctttaact accagggtgt taacgacttc gacttcgact tcaaacagtt
    22561 tctgcacagc agctttgatt tctgctttgg tcgcgtcttt agcaactttg agtacgatgg
    22621 tgttagattt ttccatcgca gtagacgctt tttcagaaac gtgcggtgca cgcagcacct
    22681 tcagcagacg ttcttcacga atcatgccag catctcctca acttgcttaa cagcatcagc
    22741 agtcattacg actttgtcga aggcgatcag gctaaccggg tcgataccag ttgcatcgcg
    22801 tacgtcaacc ttgtgcaggt tgcgcgcagc caggaacagg ttttcgtcca gctcaccggt
    22861 gatgatcagc acatcttcca gagccatgtc tttcagtttc tgtgccagca gcttagtttt
    22921 cggcgcttct acagagaact tctcgacaac gatcagacga tcctgacgta ccagttcgga
    22981 caggatgctt ttcagcgcgc cgcggtacat cttcttgtta actttttgac tgtggtcctg
    23041 cggacgagca gcaaaggtca cgccaccaga acgccagatc gggctcttga tagaaccaga
    23101 acgcgcacgg ccggtgcctt tctggcgcca cggtttttta ccggaaccag ttacttcagc
    23161 acgagtcttc tgagcacgag taccctgacg agcaccagct gcataagcaa caacaacctg
    23221 gtgaaccagc gcttcgttga aatcacgacc gaaggtagtt tcggaaacag tcagcgcgct
    23281 ctgcgcgtct ttcaatacta attccattgc tatctcctta cgccttcaca gctggtttaa
    23341 cgatcaggtc gctaccggtt gcacccggga cagcaccttt aaccagcagc aggttgcgct
    23401 cagcgtcaac gcgtactacg tcaaggctct gaacggttac acgttcgtta cccatctgac
    23461 ctgccatttt cttgcctttg aacactttgc ccggagtctg gttctgaccg atagaacccg
    23521 gaacgcggtg agacaaggag ttaccgtgag tagcgtcctg ggtacggaag ttccagcgct
    23581 taacggtacc tgcgaaacct ttacctttag aggtgccagt tacgtcaact tttttaacgt
    23641 cagcaaacag ttcaacgcta atgctctgac ctacagtgaa ctcttcgcct tcagccaggc
    23701 ggaattccca cagaccacgg ccagcttcta cgccagcttt agcgaagtgg ccagcttcag
    23761 gcttggtcac acggttagct tttttagcac cggtggttac ctgaatagca cggtagccat
    23821 cgttagccag gtctttaacc tgagtaacgc ggtttgcttc aacttcgatt acggttactg
    23881 ggatagaaac gccgtcttct gtgaagatac gggtcatacc cactttttta ccgactaaac
    23941 caatcattgt ttcaacctct caatcgctca atgacctgat taacccaggc tgatctgcac
    24001 gtctacaccg gcagccagat ccagacgcat cagagcatca acggttttct cggttggctc
    24061 aacgatgtca accagacgca agtgagtacg gatttcgtac tgatcgcgcg cgtctttgtt
    24121 gacgtgcggg gagatcagaa cagtgaagcg ctctttgcgt gtcggcagcg ggatcggacc
    24181 acggacctgc gcaccagtgc gcttggcagt ctcgacgatt tccgcggttg cttgatcgat
    24241 cagacgatga tcaaacgctt tcaggcggat acggattctt tggttctgca tgagaccaga
    24301 gctccaatta ttttataaac gaaaatgatt actcctcaga cccattacga ttgatgggag
    24361 agtgtaaccg ttcttacgta gctccccgat tgggagcatt gttaggtagc caaattcggc
    24421 taactgaggt tcagattgaa cctgctgtca actacgacaa gcccgcgcat tatacatact
    24481 taaccaccga acgcaagcgt attgctagta aagtaaacag ccttcgggtt atctctcaag
    24541 ttcgttcagc gcatttttga cacgctctct taaaccggct ttctcgtcgc tttcttctac
    24601 agtattttct tcgtcttcag aagaagaggg agcattctca accgcgacgt ctgggtcatc
    24661 ttcctcctcg acggattcga cgacggggag tggctgtttt ttgaagaaat agtgttgtgt
    24721 tgattgttta aaagcgggcg tttcagcctc tacagtgact tctgctttta ctatccaaag
    24781 cgcccatagc tgcctggcat acccaccaac caacaccatt cctgcaaacc acaggcaaat
    24841 cagcaataaa caaatgacat atattatggt gctgtgtttt tgtcgggaga agataccagg
    24901 gtgacctgtt tctttctgct cacgcgcggc gatatagaat tcaaacatgt catttcccct
    24961 tcactgttgt ttccggggaa atatttgctt catcccgata taagtaggca ctctcccctg
    25021 ccacctgcca gagatgtact aacgttgaaa agccaacaac accgtcagtt ttcaagtggt
    25081 ggcttttttg aaattgcttt attttttcta ccaacaaggg acgccactct gccgaaggtt
    25141 ctgtcgaaat atgcaatgcg cggtttaaca tcgtttccag ccagagaatc tcttcctcgc
    25201 tgctatcacg cgtaatggta ctctcccctt ccggcgacat tttccataac agaagataat
    25261 cacccgtcca taccgactca aaccattgat gtgtcagcgt ccaggtttgc tgccctacga
    25321 ggacatcaac ggtagcatca ccgacgcgaa cgaccacaac aggcaatttt ttgtccccga
    25381 ctttaagcga tgcgatccag ggcaaattct gatctaccaa tgtttgtaaa gaagcattcc
    25441 cacttttaca tgccagcccc gctcttactg cctgatcgca ccaggccgaa tcagctggga
    25501 cctcgtaccc ccatacgccg tataacacgc ttagcgcctc actatcgcgc atcactacat
    25561 gagcaatatc cttagtcata tcttgtttaa ctactggcgt tacgggtacc agccacgcag
    25621 gaacggggaa cggatctgtg aaagaggaca gcagtagcca accacaggta agtgcgataa
    25681 tggttccact catcaccacg gcaagctgca ttttccgggg gcggttatca cctaatacgc
    25741 ggtaaatcat tcgccaatgt tttgcagtga aaacgctggt ctgctcggct tgctgtaata
    25801 agctaattgt ttcgccggca agcgccagct ttctgcgatc tccccgacat aataaagcta
    25861 tccgtttcag aactctggca gttaaaagga gatcgggctg gtcaatattc tgaccttcaa
    25921 ggatgcttgc cttaagctcg tgctccatca gacgtcctgt tgagacaagt tcaatgccct
    25981 gttttttaaa atcacgttcg acttttcgat cgggtctgcc gatcagcaaa aattgaccgt
    26041 caggaataag agatatcgcg cgttggagat catcaagtat gcggcggtga ttcaggtgag
    26101 cattttcaat aataataaga gggcagcgac tatcagttcg atgaagcgca tgcagtaatt
    26161 gacccgccat ctcatctcgc gtccaggcgc tgtcacggca gccctgttca gccgcattat
    26221 cgtcggtaac gatccatgcg gccttacctt gcgaggaaac aacgaggctt aaagtaatca
    26281 cacggcgatg tttatctttt tgtagttgct gcgccagcca ggtttttcca cttccagcct
    26341 cacccaacaa atagcacaga cgccaggtct gacgtttctc acgcaaccag gaaagaataa
    26401 cttctcttct cgtagacata gaacttcctg ttttaattat tgattatcct taatcataaa
    26461 aataatgtat gcatttatta attaaataca tattactaat ataaataaat tttgcttgat
    26521 tcatgcaagc ggcattaata ctatttatac taacgtcaat atacaaccca cccaatctat
    26581 tttattagaa catacatcgt gcccacacta cgcttcccct tccatctggc gaatcataat
    26641 aaagatgctg ccatcaatat attgattata tttataagca tagggttgat tatttttaat
    26701 gtgaattatt tccatacaac tatagttaaa aatggacaga tcattaatca accaacaaac
    26761 gcatttcagt ctgagttcag ccttgccgct ttatggcgta acgaaaatca tgctggggta
    26821 aaagatgcaa atcccgtggc agttaatcag gaaacaccta agctctctat agcgcttaat
    26881 ggcattgtac tcacatcgaa tgatgagact tcttttgtct taattaatga ggggagcgaa
    26941 caaaagcgat actccctcaa cgaagccctt gagtcagcgc cgggaacgtt tattagaaaa
    27001 ataaataaaa ccagcgttgt gtttgaaact cacgggcatt acgaaaaagt aactctccat
    27061 ccagggctac ctgacatcat caagcagcct gattcagaaa gtcaaaatgt actcgctgat
    27121 tatattatcg ccacgccgat acgtgatgga gaacagatat acgggcttcg attgaaccct
    27181 cgtaagggcc tcaatgcatt taccaccagc ttgctacaac caggggatat cgccctcagg
    27241 atcaataatc tttcgctgac ccaccctgat gaggtttcac aggcattaag tttactgcta
    27301 actcaacaaa gtgctcagtt tacaattcgt cgcaacggcg taccccgctt gataaatgtt
    27361 tccgtcgcgg aacttacagg aatgaatgga cagcgtcatg aaaggactca ataaaatcac
    27421 ctgctgcttg ctggcagcac tactcatgcc ttgtgcaggg cacgctgaga acgaacaata
    27481 cggcgctaac ttcaataacg ccgatatccg ccagttcgtg gaaatagtgg gtcagcatct
    27541 tggcaaaacg atcctgatcg acccttcggt acagggaacc atttccgtac gcagtaatga
    27601 tacgtttagc caacaggagt actaccagtt ctttttaagt attcttgatc tttacggtta
    27661 ttccgtgatc acgctggaca atggttttct gaaagtggtg cgctcagcta atgtaaaaac
    27721 atcgccaggg atgattgctg acagttctcg tccaggcgta ggtgatgagt tggtcacccg
    27781 aatcgtaccg cttgagaacg ttcctgctcg tgacctggct cccctgctcc gccagatgat
    27841 ggatgcgggt agcgtcggta atgttgtgca ttatgaaccc tccaacgttc ttattctgac
    27901 cggtcgtgcc tccaccatta ataaactgat tgaagtcata aagcgcgttg atgtcatcgg
    27961 cacagagaag cagcaaatta ttcatctgga atatgcgtca gcggaagatc tcgccgaaat
    28021 tttgaatcaa ttaatcagcg aaagccacgg taaaagccag atgcctgccc tcctttccgc
    28081 gaagattgtg gcggataagc gaaccaactc tcttatcatc agcgggccgg aaaaagcacg
    28141 ccagcgcatc acttcattac tgaaaagcct tgatgtagaa gagagcgagg aaggaaatac
    28201 tcgggtttat tacctgaaat atgctaaagc cacgaatctg gtggaagtgc taaccggtgt
    28261 ttccgaaaag ctgaaagatg aaaaagggaa tgagcgtaag ccctcctctt ctggcgcgat
    28321 ggataacgtc gccattaccg ccgatgaaca gactaactct ctggtcatta ccgctgacca
    28381 gtccgtccag gaaaaactcg ccacggtaat tgcgcgtctg gacattcgcc gtgcacaggt
    28441 gctggttgag gcaatcatcg ttgaagttca ggatggaaat ggacttaacc tcggcgtgca
    28501 atgggcgaat aaaaacgttg gagcacagca atttaccaat accggattac cgatttttaa
    28561 cgctgcgcaa ggtgtggctg attataaaaa gaatggtggg atcaccagcg cgaatcctgc
    28621 ctgggatatg tttagcgcct acaatggcat ggccgcaggc tttttcaatg gcgactgggg
    28681 cgtattgttg actgcgctgg ccagtaacaa taaaaatgac atcctcgcca ccccgagcat
    28741 cgtaacgctg gataataaac tcgcgtcctt caacgttggt caggatgtgc cggtgctatc
    28801 tggctctcag accacttctg gggataacgt ctttaatact gtcgaacgca aaacggtggg
    28861 cacaaaactc aaagtcactc cgcaggtcaa tgaaggcgac gcggtactgc tcgaaataga
    28921 gcaggaggtt tccagcgttg actcttcgtc taattcgacg ctcggcccga catttaacac
    28981 ccgaactatt caaaatgccg tgctggtcaa aaccggtgaa acggtggtcc tgggcggatt
    29041 gctggatgat ttttctaaag agcaagtgtc aaaggttcct ctgcttggcg atattccttt
    29101 agtggggcaa ctcttccgct atacttcgac cgagcgcgct aaacgcaacc tgatggtatt
    29161 tatccgccca acgattatcc gtgacgatga tgtttatcgc tcactgtcaa aagagaaata
    29221 cacccgctac cgtcaggagc aacaacagcg aatcgacggg aaatcaaaag cgctggttgg
    29281 ctcggaagat ttgccggtgc tggatgagaa cacgttcaac agccataccc ctgcgccctc
    29341 ggcacggtaa ggcattctta tgagaattca ctcaccgtac cccgccagtt gggcgctggc
    29401 acaacgaatt ggttatctct attcagaggg cgagattatt tatctcgccg atacgccatt
    29461 cgaacggtta ctcgatattc aacgtcaggt tggccagtgc cagaccatga ccagcttgtc
    29521 acaggctgat tttgaagctc ggctggaagc ggtgttccat cagaataccg gtgagtcgca
    29581 acagattgcg caggatatcg atcaatccgt cgatcttctc tcgctttcgg aagagatgcc
    29641 cgcaaatgaa gatctcctga atgaagattc agcggcaccg gttatccgct tgatcaatgc
    29701 gattttgagt gaggccatca aagaaaccgc ctccgatatc cacattgaaa cctatgaaaa
    29761 aacaatgtcg atccgttttc gcatcgacgg tgttttgcgg acaattttac agccaaacaa
    29821 aaaactggcg gcactgctta tctcccgaat taaggtcatg gctcgtcttg atatcgccga
    29881 aaaacgtatt ccacaggatg gacgtattag tttgcgtatc gggcgacgta acatagatgt
    29941 ccgcgtatcc acactgccgt ccatctatgg tgaacgcgcc gtgctccgtc tgctggataa
    30001 aaacagcctc cagctttcat tgaacaacct ggggatgacg gcagcggata aacaggattt
    30061 agaaaatctc atccagcttc cacacggtat tatcctggtg accggaccga caggctccgg
    30121 taaaagcacc acgctctacg ccatcctttc ggcgctgaat actcccggcc gcaatattct
    30181 gacggtagaa gatcccgtgg aatatgagct ggaaggcatt gggcaaacgc aggtgaatac
    30241 ccgtgtggat atgtctttcg ctcgcggcct gcgcgccata cttcgccagg acccggatgt
    30301 cgtcatggtg ggggaaattc gtgatacaga aaccgcgcag attgcggttc aggcctcgct
    30361 caccggccat ctggtactct caacactcca cactaacagt gcatcaggcg cagtgacccg
    30421 gctccgcgac atgggcgtcg aatcattcct gctttcgtct tccctggcag ggattatcgc
    30481 gcaacgtctg gttcgtcgcc tgtgtccgca atgccgacaa ttcacgcccg tatcacccca
    30541 acaagcgcag atgtttaaat atcatcagct cgcggtgaca acaattggca ctcccgtagg
    30601 ctgccctcat tgccatcaat ccggctatca ggggcgcatg gcgatccacg aaatgatggt
    30661 ggtgacgccg gaattacggg ccgctattca tgaaaatgtg gatgaacaag cactggagcg
    30721 actggtccgg caacaacaca atgccttaat caaaaatggc ctgcaaaaag tgataagcgg
    30781 tgacacctcc tgggatgagg ttatgcgcgt cgccagtgcc acgctggaga acgaagcatg
    30841 aattatcgct atcgcgccat gacccaggat ggtcaaaaat tgcaggggat cattgatgct
    30901 aacgatgaac gtcaggcacg actgcggctg cgtgaagaag ggcttttcct gctggatatt
    30961 cgcccccaaa aaagttcggg agtaaaaaca cgtcgcccga ggatcagcca tagtgaactg
    31021 acgcttttca cccggcagtt ggcaacctta agcgcagcgg cattacctct ggaagagagc
    31081 cttgccgtaa tcggtcaaca aagcagtaat aaacgactgg ctgatgtgtt aaatcaggta
    31141 cgcagcgcca tccttgaagg gcatcccctt tccgatgcgt tacaacattt tcccacgctt
    31201 ttcgattcgc tctaccgtac cctggttaaa gcgggcgaaa agagcgggct gctggccccg
    31261 gtgttggaaa aactggctga ttacaatgaa aatcggcaga aaatccgcag caaactcatt
    31321 cagtcgctga tctacccctg catgctgact gcggtggcga ttgtggtcgt gattattctc
    31381 ctcactgctg tcgtgcccaa aattaccgaa cagttcgtgc atatgaagca gcaactgccg
    31441 ctgagtacac gcattctttt aggtctgagc gacacgttgc aacataccgg cccgacatta
    31501 ctagcgacag tgtttattgt cgctgtaggt ttctggctct ggttaaaacg cggcaataac
    31561 cgccaccgtt ttcatgccat gttgctgcgc gttgcactca tcggcccgct gatttgcgcc
    31621 attaacagcg cacgctatct ccgcacttta agtattttgc aatccagcgg cgtccctctg
    31681 ctggatggga tgaatttgtc caccgaaagt ctcagcaacc tcgaaatccg caagcgtctg
    31741 gcaaatgcgg cagaaaacgt tcggcagggt aacagcattc atctttcgct ggaacaaacg
    31801 gcaatcttcc cgccgatgat gctctacatg gtggcctctg gcgaaaaaag cgggcagctc
    31861 ggcacgttaa tggtcagagc agcagataac caggagacac tccaacaaaa tcggatcgcc
    31921 ttaacgctct ccatcttcga gccagcactc attattacga tggcactgat cgtcctgttt
    31981 attgtcgtct cggtactcca acctcttctt caacttaact caatgattaa ttaaggaaag
    32041 tatatgcgcg caacggataa gcaacgcggt tttacattac tggaaattat ggtggtgatc
    32101 gtcatcattg gcgtacttgc cagcctggtg gttcccaacc tgatgggcaa taaagaaaag
    32161 gcggataaac aaaaagccgt cagcgatatt gtggcgctgg aaaacgccct ggatatgtac
    32221 aagctcgaca atcatcgcta tccaaccaca aatcagggac ttgaatcctt agtcgaagcg
    32281 ccgacactgc caccgctggc cgcaaactat aacaaggaag gttatatcaa gcgtctgcct
    32341 gccgatccct ggggcaatga ttatgtcctc gttaatcctg gtgaacatgg tgcatacgat
    32401 ctgctttcag cagggcccga tggtgaaatg ggaaccgagg acgacatcac caactggggt
    32461 ttgagcaaga agaaaaagta agtcagcgat gaatcagcaa cgcgggttta ccctgctgga
    32521 gatgatgctg gtgctggcgc tggtcgctat cacggcaagt gtggtgctct tcacatatgg
    32581 tcgggaggat gccgccaaca ctcgtgcgcg cgaaacagcg gcgcggttta ccgctgcact
    32641 tgaattagcc atcgaccgcg ctacgctcag tggtcagcct gtcggtatcc atttctctga
    32701 ttcagcatgg cgcattatgg taccgggcaa aacaccatca gcctggcgct gggttccatt
    32761 acaagaagat gccgcagacg agagcaaaaa tgactgggat gaagaactct caattcatct
    32821 tcagccattt aaacctgatg acagcaacca accgcaggtg gtgattctgg ccgacggaca
    32881 aatcacgccc ttctctttgt tgatggctaa cgcagggacc ggtgagccgc tcctgacgtt
    32941 agtctgttca ggatcatggc ccctggatca aacgctcgcc agagataccc gcccatgaac
    33001 aaacaatcag ggatgacact gcttgaagtc ttactggcga tgagtatttt cgccactgtt
    33061 gcgctggctt taatgagcag catgcaaggg caacgaaatg ccatcgagcg gatgcgtaat
    33121 gagacgctgg cgctgtggat cgcagataac cagcttaagt cgcaagatac attcaacgat
    33181 gaaaacacgt ccagttcagg caaagagata attaatggcg aagagtggaa ctggcgcagc
    33241 gatgtccatt caagcaaaga cggcacgctt ctggaacgca ccataacggt gacgttaccc
    33301 aacggtcaga aaaccgctct gactcgttat cagagcatcc atgatagatc cgggcaggcg
    33361 caagatgatt aatcgccaac agggtttcac attgctggaa gtcatggcgg cactggcgat
    33421 cttctcaatg cttggcgtgc tggcgtttat ggttttttcg caagcgtcta cgctacatca
    33481 acgctcacag aaagaaatcc agcagttcaa tcaactgcaa cgcaccatca ccattcttga
    33541 taacgatctc ctgcaactgg tcgctcgccg aaatcggagt acggataaaa tcatggtgtt
    33601 gggtgaagag gctattttta ccacgcaaag tcgcgatccg caggccccgc tcaatgaagc
    33661 gcaaacctta caaacggttc actggtatct gcgaaatcat acgctttacc gtgctgttcg
    33721 tacttctgtg gatggcagga agaatcaacc tgcccaggcg atgctggagc atgtcgagag
    33781 ctttcttctg gaaagtaaca gcggggaaag ccaggagctt cctctctcag tcacgttaca
    33841 tctgaagact cagcaatatg gggcgttaca gcgccgtttt gcattacctg agcagttagc
    33901 gagagaagag tcgccagctc agacgcaggc cgggaataat aaccatgaat aatgaacaac
    33961 gcggcgtagc actgctcatt gtactcatgt tactggcgct catggctgct ctggctgcag
    34021 acatgacgat cagttttcac ggccagcttc atcgaacccg ccaggtaaac catcatttgc
    34081 aacggcaata tgatattgag ctggcggaga aacttgcgct tgccagcctc accctggacg
    34141 ttaaagataa cgacagacaa accacgttgc agcagttctg gccacaaccg caacagttgc
    34201 agctggaaaa tggcaataca gtgaagtggc agttacgcga tgcccagcac tgttttaatc
    34261 tcaatgcctt agcaaaaatc tctgacgccc cgctggcttc ccctgatttc cccgcccagg
    34321 tattcagtgc cctgctgatt aacgccggta tcgatcgcgg aaacaccgat gagatcgtgc
    34381 aatcaattgc tgactatatt gatgtcgatg actcaccacg ctttcacggc gcagaagata
    34441 acttttacca aagccagacg ccgccccggc atagtgccaa tcagatgctt tttctgaccg
    34501 gcgaattgcg tcagataaaa ggcataaccg aaaacatcta tcagcggctt atcccgtatg
    34561 tctgcgttct cccaaccacg gaacttagca tcaatcttaa tatgcttacg gagaacgata
    34621 ttccgctctt cagagcgtta ttcctcaata acatcacgga tgctgacgct cgggtcttat
    34681 tacaaaaaag gcccagggaa ggctggctaa ccacggatgc atttctttac tgggcgcagc
    34741 aggatttctc cggcgtgaag cctctggtag ctcaggtgaa agggcatctc tttccctata
    34801 gccgctattt tacgctgagt acggaaagta tcagcgacga acaatcccag ggatggcaaa
    34861 gccatatttt cttcaaccgt aaacagcaga gcgcccaaat ctatcggcgt acgctgcaac
    34921 tctactaatc ataaggatcg tgatgcctga atcgttgatg gtcatacgtt cttcctcgac
    34981 gctgcgtaag cattgggaat ggatgacgtt ctccgcagat agcgtatctt cggtacatac
    35041 gcccacggac gatcttccgc tggagtcgct ggccgatcag cccggtgccg gaaatgttca
    35101 tcttttgatc ccaccagagg gccttttata tcgttccctc acgctgccca acgccaaata
    35161 caaactcacc gcacaaacat tgcaatggct ggcagaggag acgttacccg acaacaccca
    35221 ggactggcac tggacggttg tcgataaaca aaacgaaagt gtcgaggtga taggcattca
    35281 gtcggagaaa ctcagtcgct atcttgagcg tttacatact gccggactca acgtgacgcg
    35341 ggttttgcca gatggctgtt atcttccctg ggaagtagat agctggactc tggttaacca
    35401 gcaaacaagc tggcttattc gcagtgccgc acatgccttc aatgaactgg atgaacactg
    35461 gttacagcat cttgctgccc agtttccgcc tgagaatatg cgttgttatg gtgtggcacc
    35521 tcacggcgtt gcagcgacca atccacttat ccagcatcct gaaataccat cactgagttt
    35581 gtattctgcc gatattgcat tccagcgcta tgacatgctg catggtgttt ttcgtaaaca
    35641 gaaaactgta agcaaatcgg gaaaatggct cgcccgcctg gcagtaagct gtctcgttct
    35701 cgccattctt tcttttgttg gcagccgggg catcgcgctc tggcaaacgc ttaagattga
    35761 agatcaactt cagcatcaac agcaagaaac ctggcaacgt tatttcccgc agatcaaacg
    35821 cacccacaac tttcgttttt actttaagca gcaactcgct cagcaatatc ctgaagctgt
    35881 gccgttgctc tatcatttac aaacacttct gctggaacac cctgaactgc agttaatgga
    35941 ggcgaactat agccagaagc aaaagtcgct gaccttaaaa atgagtgcta agagtgaagc
    36001 caatatcgat cgtttctgtg aattaacaca gtcctggctt ccgatgcaaa aaacagaaaa
    36061 agatccggtc agtggtgtat ggacagtaag gagctcaggg aaatgattaa atcatggtgg
    36121 gcagaaaaat ccacatcgga aaaacagctc gttgcggcgc tggcggttct ctcgctcggc
    36181 gttttttgtt ggcttggggt gataaaaccg atagatacct acattgcaga gcatcagtcc
    36241 cacgcgcaaa aaattaagaa ggacatcaaa tggatgcagg atcaagcgag cacccatggt
    36301 ttactcggtc atcctgcgct tacgcagccc atcaaaaata tccttctcga ggaagcaaaa
    36361 cgtgagaatt tggctatcac gctggaaaac ggtccggaca atactctgac aattcatcct
    36421 gtcacagctc ccctcgaaaa cgtctcccgc tggctcacta cggcacagat tacatacggt
    36481 attgttattg aagaccttca gtttactctt gccggaaacg aacagatcac cctcaagcat
    36541 ttgtccttca gggagcaacc ataatgacaa tgctactgcc gctcttcatt ctggttggtt
    36601 ttattgcagg ttattttgtt aatgccatgg cctatcacct ctcgccgttg gaggataaaa
    36661 cggcgttaac gtttcgccag gtattggttc atttcaggca aaaaaaatat gcctggcatg
    36721 atacagtgcc cctgatactt tgcgttgctg ctgctatcgc ctgcgccctg gcacctttca
    36781 cgcccatcgt gactggcgca ctctttctct atttctgttt cgcactcacg ctcagtgtta
    36841 ttgattttcg cactcagctc ctgcccgaca aactcacctt accgctgctc tggcttggct
    36901 tggtatttaa tgcgcagtct ggattaattg atttacatga tgcggtttac ggcgcggtag
    36961 ctggctatgg ggtgctgtgg tgtgtttact ggggcgtctg gttagtttgt cacaaagagg
    37021 gattgggcta cggtgatttc aagctactgg ccgctgcagg cgcgtggtgt ggctggcaaa
    37081 cgttgccaat gatactgcta attgcctcac tgggtggcat tggttacgcc atcgtttcac
    37141 aacttctgca acgccggact ataaccacta tcgcattcgg cccgtggctt gcgctcggca
    37201 gcatgataaa cctggggtat ctggcctgga tctcgtatta accgggaggg ttctccctcc
    37261 cgacacggct caactttctt cgcggatctg tgcttgcaga taattttgca ggcccatctt
    37321 ctgaatcaga tcaagttccg tttccagcca gtcgatatgg ccttcttcat cacgcaaaat
    37381 ttctatcatc atatcgcggc tgacgtaatc atgaacgcta tcggcataac caattgcctc
    37441 acgcaaattc ttcgcgccat ccagctcaag tgccagatca gaacgcagca tttcctcaac
    37501 atcctcacca atgttcagtt tgcccaggtc ctgtaaattt ggaagacctt ccagaaaaag
    37561 aatgcgctca atataacgat cggcgtgttt catctcatca atggattcat gatactccac
    37621 atcattgaga cgtttgagac cccagttttt aaacatccgg gcatggagaa agtactgatt
    37681 gattgcgaca agctcatttc ccaacagttt gttgagataa tttataacct tagtatcacc
    37741 tttcattttt tgactccttc gcttccactc tttgaagcgt agaacggctt acgagtaagt
    37801 caaaaaacac actccgttta tgcggactcc ttaaactccg gcaactgcat taattcatcc
    37861 tccatcactt cacgcgcggc ccggacacat ttaccgcact gattgccgac cggaataaat
    37921 ttttttaatt gctggaacga gtgaggggaa aactggcgca cagcctgacg aattttttta
    37981 tcgctgatac ca
//
LOCUS       DAZGTJ010000042        37225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000042 DAZGTJ010000000
VERSION     DAZGTJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.042"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..121
                     /gene="yecU"
                     /locus_tag="ACHK8E_002620"
     CDS             <1..121
                     /gene="yecU"
                     /locus_tag="ACHK8E_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP0344868.1"
                     /translation="TLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            108..332
                     /gene="yecF"
                     /locus_tag="ACHK8E_002621"
     CDS             108..332
                     /gene="yecF"
                     /locus_tag="ACHK8E_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP0344869.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(399..1121)
                     /gene="sdiA"
                     /locus_tag="ACHK8E_002622"
     CDS             complement(399..1121)
                     /gene="sdiA"
                     /locus_tag="ACHK8E_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP0344870.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1351..2103)
                     /gene="tcyN"
                     /locus_tag="ACHK8E_002623"
     CDS             complement(1351..2103)
                     /gene="tcyN"
                     /locus_tag="ACHK8E_002623"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP0344871.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2100..2768)
                     /gene="tcyL"
                     /locus_tag="ACHK8E_002624"
     CDS             complement(2100..2768)
                     /gene="tcyL"
                     /locus_tag="ACHK8E_002624"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP0344872.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2783..3769)
                     /gene="dcyD"
                     /locus_tag="ACHK8E_002625"
     CDS             complement(2783..3769)
                     /gene="dcyD"
                     /locus_tag="ACHK8E_002625"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP0344873.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3874..4674)
                     /gene="tcyJ"
                     /locus_tag="ACHK8E_002626"
     CDS             complement(3874..4674)
                     /gene="tcyJ"
                     /locus_tag="ACHK8E_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0344874.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4762..5313)
                     /gene="fliZ"
                     /locus_tag="ACHK8E_002627"
     CDS             complement(4762..5313)
                     /gene="fliZ"
                     /locus_tag="ACHK8E_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP0344875.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5359..6078)
                     /gene="fliA"
                     /locus_tag="ACHK8E_002628"
     CDS             complement(5359..6078)
                     /gene="fliA"
                     /locus_tag="ACHK8E_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP0344876.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6397..8136)
                     /locus_tag="ACHK8E_002629"
     CDS             complement(6397..8136)
                     /locus_tag="ACHK8E_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP0344877.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            8402..9808
                     /gene="fliD"
                     /locus_tag="ACHK8E_002630"
     CDS             8402..9808
                     /gene="fliD"
                     /locus_tag="ACHK8E_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0344878.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9833..10243
                     /gene="fliS"
                     /locus_tag="ACHK8E_002631"
     CDS             9833..10243
                     /gene="fliS"
                     /locus_tag="ACHK8E_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0344879.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            10243..10608
                     /gene="fliT"
                     /locus_tag="ACHK8E_002632"
     CDS             10243..10608
                     /gene="fliT"
                     /locus_tag="ACHK8E_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP0344880.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10686..12173
                     /gene="amyA"
                     /locus_tag="ACHK8E_002633"
     CDS             10686..12173
                     /gene="amyA"
                     /locus_tag="ACHK8E_002633"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0344881.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12207..12620)
                     /gene="yedD"
                     /locus_tag="ACHK8E_002634"
     CDS             complement(12207..12620)
                     /gene="yedD"
                     /locus_tag="ACHK8E_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP0344882.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12807..14012
                     /gene="yedE"
                     /locus_tag="ACHK8E_002635"
     CDS             12807..14012
                     /gene="yedE"
                     /locus_tag="ACHK8E_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP0344883.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            14009..14242
                     /gene="yedF"
                     /locus_tag="ACHK8E_002636"
     CDS             14009..14242
                     /gene="yedF"
                     /locus_tag="ACHK8E_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP0344884.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14351..15019
                     /gene="yedK"
                     /locus_tag="ACHK8E_002637"
     CDS             14351..15019
                     /gene="yedK"
                     /locus_tag="ACHK8E_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP0344885.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            15130..15609
                     /gene="yedL"
                     /locus_tag="ACHK8E_002638"
     CDS             15130..15609
                     /gene="yedL"
                     /locus_tag="ACHK8E_002638"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0344886.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            15849..15989
                     /locus_tag="ACHK8E_002639"
                     /pseudo
     CDS             15849..15989
                     /locus_tag="ACHK8E_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(16014..16328)
                     /gene="fliE"
                     /locus_tag="ACHK8E_002640"
     CDS             complement(16014..16328)
                     /gene="fliE"
                     /locus_tag="ACHK8E_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0344887.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            16543..18201
                     /gene="fliF"
                     /locus_tag="ACHK8E_002641"
     CDS             16543..18201
                     /gene="fliF"
                     /locus_tag="ACHK8E_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0344888.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            18194..19189
                     /gene="fliG"
                     /locus_tag="ACHK8E_002642"
     CDS             18194..19189
                     /gene="fliG"
                     /locus_tag="ACHK8E_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0344889.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            19182..19868
                     /gene="fliH"
                     /locus_tag="ACHK8E_002643"
     CDS             19182..19868
                     /gene="fliH"
                     /locus_tag="ACHK8E_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0344890.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            19868..21241
                     /gene="fliI"
                     /locus_tag="ACHK8E_002644"
     CDS             19868..21241
                     /gene="fliI"
                     /locus_tag="ACHK8E_002644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0344891.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            21260..21703
                     /gene="fliJ"
                     /locus_tag="ACHK8E_002645"
     CDS             21260..21703
                     /gene="fliJ"
                     /locus_tag="ACHK8E_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0344892.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            21700..22827
                     /gene="fliK"
                     /locus_tag="ACHK8E_002646"
     CDS             21700..22827
                     /gene="fliK"
                     /locus_tag="ACHK8E_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0344893.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            22932..23396
                     /gene="fliL"
                     /locus_tag="ACHK8E_002647"
     CDS             22932..23396
                     /gene="fliL"
                     /locus_tag="ACHK8E_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP0344894.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            23401..24405
                     /gene="fliM"
                     /locus_tag="ACHK8E_002648"
     CDS             23401..24405
                     /gene="fliM"
                     /locus_tag="ACHK8E_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP0344895.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            24402..24815
                     /gene="fliN"
                     /locus_tag="ACHK8E_002649"
     CDS             24402..24815
                     /gene="fliN"
                     /locus_tag="ACHK8E_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP0344896.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            24818..25183
                     /gene="fliO"
                     /locus_tag="ACHK8E_002650"
     CDS             24818..25183
                     /gene="fliO"
                     /locus_tag="ACHK8E_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP0344897.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            25183..25920
                     /gene="fliP"
                     /locus_tag="ACHK8E_002651"
     CDS             25183..25920
                     /gene="fliP"
                     /locus_tag="ACHK8E_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0344898.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            25930..26199
                     /gene="fliQ"
                     /locus_tag="ACHK8E_002652"
     CDS             25930..26199
                     /gene="fliQ"
                     /locus_tag="ACHK8E_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP0344899.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            26208..26993
                     /gene="fliR"
                     /locus_tag="ACHK8E_002653"
     CDS             26208..26993
                     /gene="fliR"
                     /locus_tag="ACHK8E_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0344900.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            27283..27906
                     /gene="rcsA"
                     /locus_tag="ACHK8E_002654"
     CDS             27283..27906
                     /gene="rcsA"
                     /locus_tag="ACHK8E_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0344901.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(27950..28138)
                     /gene="dsrB"
                     /locus_tag="ACHK8E_002655"
     CDS             complement(27950..28138)
                     /gene="dsrB"
                     /locus_tag="ACHK8E_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP0344902.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            28301..28528
                     /gene="yodD"
                     /locus_tag="ACHK8E_002656"
     CDS             28301..28528
                     /gene="yodD"
                     /locus_tag="ACHK8E_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP0344903.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            28827..29642
                     /gene="yedP"
                     /locus_tag="ACHK8E_002657"
     CDS             28827..29642
                     /gene="yedP"
                     /locus_tag="ACHK8E_002657"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP0344904.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(29639..31333)
                     /gene="dgcQ"
                     /locus_tag="ACHK8E_002658"
     CDS             complement(29639..31333)
                     /gene="dgcQ"
                     /locus_tag="ACHK8E_002658"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP0344905.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(31504..31686)
                     /gene="yodC"
                     /locus_tag="ACHK8E_002659"
     CDS             complement(31504..31686)
                     /gene="yodC"
                     /locus_tag="ACHK8E_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP0344906.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(31765..32682)
                     /gene="yedI"
                     /locus_tag="ACHK8E_002660"
     CDS             complement(31765..32682)
                     /gene="yedI"
                     /locus_tag="ACHK8E_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP0344907.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            32855..33775
                     /gene="yedA"
                     /locus_tag="ACHK8E_002661"
     CDS             32855..33775
                     /gene="yedA"
                     /locus_tag="ACHK8E_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP0344908.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(33764..34234)
                     /gene="vsr"
                     /locus_tag="ACHK8E_002662"
     CDS             complement(33764..34234)
                     /gene="vsr"
                     /locus_tag="ACHK8E_002662"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP0344909.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(34215..35633)
                     /gene="dcm"
                     /locus_tag="ACHK8E_002663"
     CDS             complement(34215..35633)
                     /gene="dcm"
                     /locus_tag="ACHK8E_002663"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP0344910.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(35700..36395)
                     /gene="yedJ"
                     /locus_tag="ACHK8E_002664"
     CDS             complement(35700..36395)
                     /gene="yedJ"
                     /locus_tag="ACHK8E_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP0344911.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(36435..36716)
                     /gene="drpB"
                     /locus_tag="ACHK8E_002665"
     CDS             complement(36435..36716)
                     /gene="drpB"
                     /locus_tag="ACHK8E_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP0344912.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         9102 a         9690 c         9358 g         9075 t
ORIGIN      
        1 cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt
       61 aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg
      121 atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga
      181 tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga
      241 tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg
      301 ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag cgtgcctttc
      361 agccggtttt tgcatctggc acgcagaaag aaaagcgatc aaattaagcc agtagcggcc
      421 gcgtaacagg ccacctgggt cttatttggt gcattgattt ttttctgcat gtttttctga
      481 tggaaattga ccgtattctc agagattgac aaaatcatcg ctatctctgc tgatgtcttc
      541 ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat ctctggcgtc
      601 atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac cagtaactgc
      661 atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgcagtgca acgggaaaag
      721 gacaaaaagc ccagcgcccg gtttggcagc attaaatact gagtgacacc gcggcgtaaa
      781 ccatgcgcgc gcgcagcttc ccataacggc tgtgcttcgc tgaataagtc atcattccac
      841 attaaatggc cctgactaaa gttttcaggg ttcagtacag gatcaattgc gagaaagttt
      901 tttgcctgat aataactaac ccacgcctca gggtaattgg tgtagaaagc cactttaggt
      961 cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc cagctgctga
     1021 gcctgaagct ctatttcatg gtagacctct tctgcggcct ccatcctctg aaaacgcaac
     1081 agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg caacgcccct
     1141 gagagtgagt atcatttata atgataattc aaactatcac ataaataaga tttatatata
     1201 atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct tatgttatta
     1261 atgagttatg ctgatttgtt aagcagtttt atcacgtttg aagtggcgca ccgccccgac
     1321 aggtgaatcg tcggggctga ttttttctta ttattgcagc agaaacttct cgaggaactg
     1381 gcgggtgcga ggctgctcgg gatcggcaaa taacgctttt gcggccccct gctcgactat
     1441 ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg
     1501 cgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt tcaggacttc
     1561 gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca cttcaggacg
     1621 catagccagc gctcgcgcaa tcgcgacacg ctgctgttga ccgccagaca aacgacgtgg
     1681 atagctggtt tctttacctg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt
     1741 ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac
     1801 cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg
     1861 gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatggtga tatcgccaac
     1921 ggtgatggtt cccgcttccg gttgttccag cagatttatg ctgcgtagca acgtggtttt
     1981 gccggaacca cttggaccga taattgccac cacttcgcca ggcttcacct caaggtcgat
     2041 accgtgcagc accgtctgac cgtggaattt tttcaccagg ttcttaactt caatggcact
     2101 cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat
     2161 aatactgtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc
     2221 agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg
     2281 gccagcgacg tatcttttac caggctgata aagctgttcg acagcggcgg caacgccaca
     2341 cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat accaatactg
     2401 gcagcagctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag tgtttcggcg
     2461 gcataggcgg cagtgtttag cgacagacca atcattgctg acggaatggg atctaattcg
     2521 atgccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag tggcgtacca
     2581 cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg cgaaaggcgc
     2641 atcagcgcga gaataaaccc gagcagtaag ccaaaaaaca tgccgccgat actgagttgc
     2701 agcgtatacc cagccccttt cagcaggaac ggcaaggaat caataaccag ttgtatactt
     2761 tcttgcatga gcgtttccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc
     2821 cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt
     2881 caatcagccc cgccattgct tttccggtat acacaggatc aagcagaatg ccttccagcc
     2941 gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa
     3001 aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct ttcgcaatcg
     3061 cctgttggag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc
     3121 caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc
     3181 cggcactgcc cgacgcgacc accaccgacg aaatattaac tgccccttca cactgttgcg
     3241 caatttccag cgcactctcc acataaccca gcgcgcccaa agcattagaa ccgccaaccg
     3301 gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct tccagttggg
     3361 cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga tccagcaaca
     3421 aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg
     3481 ccacgcagtg cagaccgatt ttcgccgcaa ctgcggcagt ctggcgcaca tggttagact
     3541 ggatcgcccc ggcagtaatc agcgtatcgg caccttcacg cagagcatct gccgcgagaa
     3601 attccagctt acgtaattta ttgccgccca ttgccatggg agtgacgtca tcccgtttga
     3661 tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggcgttggcg
     3721 cgccgataaa ctccagccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg
     3781 atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc
     3841 tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca
     3901 tttttcggaa agggcttgca gagtgccatc tttttgcatt tccgcaattg catcattcac
     3961 cgctttcagc aggtcttcat ttcctttacg cagcgccaca ccagactcct gacgggagaa
     4021 tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag
     4081 acggtcaacg aggatcgcat cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc
     4141 atcatcatag gtacgcacat cgacgccctg aacattctga cgcagccact cttcatagtt
     4201 ggtgcccaga ccgacaccca cttttttgcc tttcagatca tcagccgttt taatggtgcc
     4261 ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtacgggg tagagaaatc
     4321 gtattttttc ttgcgctcgt cagaaatggt gacctggtta atcaccacat caatgcgttt
     4381 agagtccagc gacgccagca taccgtccca tttagtcggt tttagtgacg cctcaacgcc
     4441 aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc
     4501 cccctgaaaa ctgaacggcg gataagttcc ttccagccct accagcagcg tgccgcgctc
     4561 tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatac ccgcaaccag
     4621 cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt tcatatttac
     4681 cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac
     4741 tccgctacat cttattctta tttaatatat atcagaagca ggcagactgg aggatttctg
     4801 cacaagtccg gtacacgttt gacgctgata gtgttgatac ttccgtaacg caatgcggta
     4861 attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg
     4921 cagctgatcg agggaaatat tttgctcgtg tagatgatta cccaaacggc gcagacgaac
     4981 gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa
     5041 gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca
     5101 caatgccgcc catgattttt gttccacttg ccagccattt tcatcaagca gcatgtccag
     5161 gcgggaaatc tcgattttat tcactatttt gccgtcgcga acgagggtaa tgcgatcgag
     5221 taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa gatagcggct
     5281 taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt caaaattaaa
     5341 gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatggcctg
     5401 gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa tctctttgag
     5461 attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc
     5521 gatagcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta gcgggttttc
     5581 tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc gccactcatc
     5641 gtaggagaag agctggctgt tattggtgtc gagcaacatt tggcgatagt cggcaatatc
     5701 gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag
     5761 ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc
     5821 acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa
     5881 tgccgttcct tgtagggcgt catagcgttc gacggcattg agtaacccaa tgccgcccgc
     5941 ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc
     6001 gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc
     6061 ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc
     6121 cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt
     6181 taccggtaaa ttgcgggcag aaaaaacccc gccgttggcg gggaagcacg ttgctgacaa
     6241 attaccattc atgttgccgg atgcggcgta aacgccttat ccggcctaca aaatccgcaa
     6301 attcaataaa ttgcaattca ccttgtaggc ctgataagcg cagcgcatca ggcaatttgg
     6361 cgttgccgtc agtctcagtt aatcaggtta cgacgattaa ccctgcagca gagacagaac
     6421 ctgctgcggt acctggttag cttttgccag cacggagtta ccggcctgct ggatgatctg
     6481 cgctttcgac atgttggaca cttcggtcgc atagtcggcg tcctgaatac gggactgcgc
     6541 ttcggacagg ttggtagtgg tgttgttcag gttggtgacc gcggaatcca gacggttttg
     6601 caccgcaccg agggaggagc ggaatttgtc gatggagctg atggcttcgt ccagagcttt
     6661 cagtggatcg gcggtggttg cagctttagt ttcagcgtca gtagtaaaat taccatccgc
     6721 tgaaacatag actgctttac ctgaaccatt ggtcacagtt ccgtcttttt gcaaaaacag
     6781 attggttgta ttatcggtag tcagtgaacc atcagctttg gtgtaagcgt cttcaaaaac
     6841 tgtacctgtg tattgttgta ctataccttt actatcaact ttaatatcgc ctgcgctggt
     6901 accagtagct ccaacatcaa ctgtgaatcc tgtggactta gttgccgatt gaagagactc
     6961 agcactcatt cttgcatttg ctacagtgat tgcaccagaa gtagtagcat cagtaccgcc
     7021 atccagagtg aaggttgttt catcttcagt tgtaattacc gtagatacgg cacccggtgt
     7081 gcctgttgta tgccagtttt tagtcagtgc atcaagagtg gctgctttga cagtcccagc
     7141 attgttttga gtgaggttac catctacgtc aataaatagt tctgaaccat ctttcgcggt
     7201 aattttaccg tcgctagcca atacaacgtc aattgacgta gaaccaattt taacgtttaa
     7261 gttagcagta tcgcccgctt ttggtgtcag gaaggattgt aactgagcac tgttactatc
     7321 accagaattt gtaccagctg caatagttgc actatatgta aaatcacctg tagcgctgtt
     7381 gtatgtataa tctgtagctg ttggcgatcc aaaaccattt gcaattgtgg cattaacttt
     7441 tgcgttatta gccaaactcg caattacatc tgcagctgta gatgttttca ggcctgcgct
     7501 aacagtatag gtaactgtac cattagcatc agcagtacct ggagccaaga gttgagctgc
     7561 tgccaaatca gatttagtcg ctgcagtatt agccacagcc ccgccaccat tcacattaaa
     7621 cccactcagc cccagcgtat cagagtcaat tttcttcaga tcaatagtga tagtctggcc
     7681 gtcattcgca ccaacctgaa ttttcattga accgtctttc gccagtacgt tcacgccgtt
     7741 gaactgggtc tggccagata cgcggtcaat ttcgtccaga cgggatttga tttcgtcctg
     7801 aatggagtcc agatccgaat cggagttagt cccggtagaa gcctgaaccg tcagttcacg
     7861 gatacgctgt aagttgttgt tgatttcgga cagcgcgcct tcggtggtct gtgcaacgga
     7921 gataccgtcg ttggcgttac gtgcagcttg agtcagacct ttaatgttag aagtaaaacg
     7981 gttagcaatc gcctgacctg cggcgtcatc cttcgcgctg ttaatacgca agccagaaga
     8041 cagacgctcg atagaactcg acagcgcaga ctggttcttg ttgatattat tttgagtgat
     8101 cagcgagagg ctgttggtat taatgacttg tgccatgatt cgttatccta tattgcaagt
     8161 cgttgattac gtattgggtt tccacccgtc ggctcaatcg ccgtcaaccc tgttatcgtc
     8221 tgtcgtaaaa caacctttag aatttttttc acaaacagcc attttttgtt agtcgccgaa
     8281 atactctttt ctctgcccct tattcccgct attaaaaaaa acaattaaac gtaaactttg
     8341 cgcaattcag gccgataacc ccggtattcg ttttacgtgt cgaaagataa aaggaaatcg
     8401 catggcaagt atttcatcgc tgggagtcgg gtcaggtctg gatttaagtt caatccttga
     8461 tagcctcacc gccgcgcaaa aagcgacgct aacccctatt tcaaatcagc aatcgtcgtt
     8521 taccgctaaa cttagcgcct acggtacgct gaaaagcgcg ctgacgactt tccagaccgc
     8581 caatactgca ttgtctaaag ccgatctttt ttccgccacc agcactacca gcagcaccac
     8641 cgcgttcagt gccaccactg cgggtaacgc catcgccggg aaatacacca tcagcgtcac
     8701 ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc agagacgata cgaaaacggc
     8761 gatcgccacc agcgacagta aactcatcat tcaacaaggc ggcgacaaag atccgattac
     8821 cattgatatc agcgcggcta actcatcgtt aagcgggatc cgtgatgcta tcaacaacgc
     8881 aaaagcgggc gtaagcgcaa gcatcattaa cgtgggtaac ggtgaatatc gtctgtcagt
     8941 cacatcaaat gacaccggcc ttgataatgc gatgacactc tcggtcagcg gtgatgatgc
     9001 gctacaaagt tttatgggct atgacgccag tgccagcagc aacggcatgg aggtctcggt
     9061 cgccgcccag aatgcgcagc tgacggtcaa caacgtcgcc attgagaaca gcagcaacac
     9121 catcagcgac gcgctggaaa acatcaccct gaacctgaac gatgtcacca cgggcaacca
     9181 gacgctaacc atcactcagg acacctccaa agcgcaaacg gcgattaaag actgggtgaa
     9241 tgcctataac tcgctaatag ataccttcag cagcctgacc aaatacaccg ccgtagatgc
     9301 gggagctgat agccagagtt ctagcaatgg cgcactgctc ggcgactcca cgttgcggac
     9361 gattcagacg cagttgaaat cgatgctgag taataccgtt agttcttcca actataaaac
     9421 gttggcgcag attggtatca cgaccgatcc cagcgatggc aaactggaac tggatgccga
     9481 caaactcacc gctgcactga aaaaagatgc cagcggcgta ggtgcattga ttgttggcga
     9541 tggtaaaaaa accggcatca cgaccaccat cggcagcaac ctgaccagtt ggctttcgac
     9601 aacaggcatt attaaagccg ctaccgatgg cgttagtaag accctgaata agttaaccaa
     9661 agactacaac gccgccagcg atcgcattga tgcgcaggtc gcgcgctaca aagagcaatt
     9721 tacccaactg gacgttttaa tgacctcgtt aaacagcacc agcagctact taacgcagca
     9781 gttcgaaaac aacagtaatt ccaagtaatc aatattcatc gggagacagg tcatgtacgc
     9841 ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag
     9901 ccagcaacag ctggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcgcg
     9961 cctgtttatg caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa
    10021 catcattgag aacgggctgc gggtgagtct tgatgaagag agcaaagacg aactaaccca
    10081 aaacttgatt gctctttata gctatatggt caggcgcttg ctgcaagcca atttacgcaa
    10141 cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa
    10201 agagtcttta ctctcccctt ctttgattca ggacccagac tgatgaacaa tgcaccgcat
    10261 ttgtatttcg cctggcaaca actcgtcgaa aaaagtcagc tcatgttacg cctggcgaca
    10321 gaagaacaat gggacgaact catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag
    10381 atcgcacacc tgactgaaga ggttgccccg tccaccacga tgcaggagca gctccgcccg
    10441 atgctgcgcc tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg
    10501 gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat
    10561 ggcgatcagg gtggctttgt gctggctccg caggataacc tcttttgaat cttaaagagc
    10621 caatggctcg cgaataatct gattacggct acgcttctaa tgttcccctt gaatggagtc
    10681 gaagaatgcg taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta
    10741 agctctggcc tgaactggcc gagcgcgccg acggttttaa tgatattggt atcaatatgg
    10801 tctggttgcc gcccgcctat aaaggcgcat cgggcgggta ctcggtcggc tacgactcct
    10861 atgatttatt tgatttaggc gagtttgatc agaaaggcag catccctact aaatatggcg
    10921 ataaagcaca actgctggcc gccattgacg ccctgaaacg taatgacatt gcggtgctgc
    10981 tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa agaagctatt cgcgtgcaac
    11041 gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat cattgagtgc gaaggctgga
    11101 cgcgttacac cttccccgcg cgtgccgggc aatactcgca gtttatctgg gatttcaaat
    11161 gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga tggcattttt aaaattgtta
    11221 acgactatac cggagaaggc tggaacgatc aggttgatga tgaactcggt aatttcgatt
    11281 atctgatggg tgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact
    11341 gggcgcgctg ggtgatggaa caaacgcaat gcgacggctt tcgtcttgat gcggtcaaac
    11401 atattccggc atggttttat aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc
    11461 cgctgtttat tgtggctgaa tactggtcgc atgaagtcga taaactgcaa acgtatattg
    11521 atcaggtgga aggcaaaacc atgctgtttg atgcgccgct gcagatgaaa ttccatgaag
    11581 catcgcgcat gggtcgcgac tacgacatga cgcagatctt cactgggacg ctggtggaag
    11641 ccgatccttt tcacgccgta accctcgttg ccaaccacga cacccaaccg ttgcaagccc
    11701 tcgaagcacc ggtcgaaccg tggtttaaac cgctggcgta tgccttaatt ttgttgcggg
    11761 aaaatggcgt tccttcggtg ttctatccgg acctctacgg tgcacattac gaagatgtcg
    11821 gtggtgacgg gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa
    11881 ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt atttttcgac catccgaact
    11941 gcattgcctt tagccgcagt ggcaccgacg aatatcccgg ctgcgtggtg gtcatgtcga
    12001 acggggatga tggcgaaaaa accattcatc tgggagagaa ttacggcaat aaaacctggc
    12061 gtgattttct ggggaatcgg caagagagcg tagtgaccga cgaaaacggc gaagcgacct
    12121 tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga agaggtgatt taaattcatc
    12181 cccggcggca agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc
    12241 agcgcggcgg cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac
    12301 tcaatggtgt taccgtcacg ctcaacctcg tacagctcac gtttcacggt aacgttagtg
    12361 agatcatcag acatcaatgt caacttaccc ggcacggcaa tcacccgctg ccactgacgg
    12421 caatccaggg tatcgccctc tttagtgaca atcaggctgc ctatcgcttc cggactgacc
    12481 agcgcacttt gcggcccttt ggtctgccag tagcccgcca gccagtccgg cgcgggggtt
    12541 ttcacgacat tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct
    12601 gcaattgcga gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta
    12661 aagccctgat gtcgccagcc tttccaggtt cagagagaaa tttgatctaa ctattatttt
    12721 tgtgaattat taccccgaaa ataaatttgc ttgatgtatg attcgcaact cttgtttcaa
    12781 accttctgag ttcagaggct acatacatgt catggcagca attcaaacac gcctggttga
    12841 ttaaattctg ggcccccatc cctgcggtca tcgcggcggg tattctctcc acttactact
    12901 ttggcatcac tggcaccttt tgggctgtca cgggtgaatt tacccgctgg ggcggtcagc
    12961 ttctgcagct gttcggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg
    13021 aaggatcgcc attaacccgc atcgacggca tgatgatcct cggtatgttt ggcggctgct
    13081 ttgctgcagc gctgtgggcc aacaatgtca aactacgcat gccacgcagc cgtatccgca
    13141 ttatgcaggc cattattggc ggcattatcg ctggttttgg cgcgcgtctg gcaatgggct
    13201 gtaacctggc ggcgttcttt accggcattc ctcagttctc gctgcatgcc tggttctttg
    13261 ccatcgccac tgccattggt tcatggtttg gcgcgcgctt taccctgctg cccatcttcc
    13321 gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc
    13381 aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc
    13441 tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat gctgtttggc gtcggctttg
    13501 gtttactgat tgaacgtgcg caaatctgct ttacttcggc gttccgcgat atgtggatca
    13561 ccggacgtac ccatatggcg aaagcaatca ttatcggtat ggcggtgagt gccatcggga
    13621 tcttcagtta cgtacagtta ggcgttgaac ctaaaatcat gtgggcggga ccaaatgcgg
    13681 taattggtgg tttacttttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct
    13741 ggatgtaccg cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaacgtga
    13801 tcggctcaac gattctggcg tattactggg atgatttcgc tccggcgctg gccaccgact
    13861 gggacaaaat caacctgctg aaaacctttg gtccgatggg tggcctgctg gtgacatatt
    13921 tgctgttgtt tgcagctcta atgttgatta tcggctggga aaaacgcttc ttccgccgtg
    13981 cggcaccgca gattgctaag gagatcgcat gaaaaatatc gttcctgatt accgtcttga
    14041 tatggtgggt gaaccctgcc cttatccggc tgtcgcaacc cttgaggcga tgccgcagtt
    14101 aaaaaaaggg gagatcctgg aagtggtgag cgactgtccg cagtcgatca ataatattcc
    14161 actggatgcg cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat
    14221 tcgttattta attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt
    14281 taccactcta tcacatagat tttacccgcc ccctctccta ttcttttcag tgtgctctca
    14341 ggaggtttct atgtgtggac gctttgccca atcccaaacg cgtgaagatt acctcgcgtt
    14401 tctcgcggaa gatattgaac gcgatattcc ctacgatccc gaacctattg gcagatacaa
    14461 cgtcgcgccg ggaaccaaag tccttctgct cagtgaacgt gatgaacacc ttcatctgga
    14521 tccggttttc tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg
    14581 cgtagaaact gccgcaacca gtcgcatgtt taaaccgctc tggcaacacg gtcgggcaat
    14641 ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc gacaaaaaac agccttattt
    14701 tatctatcgc gctgacggac aacctgtttt tatggccgcg ataggtagca caccgtttga
    14761 gcgtggtgac gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt
    14821 agatattcat gaccgccgcc cgctggttct gtcgccagaa gctgtacggg aatggatgcg
    14881 gcaagaggtt ggcggtaaag aagcctcaga aattgctgcc agtggctgtg tgccagcaaa
    14941 ccagttcacc tggcacccgg tatcgtgcgc ggtgggtaat gttaaaaacc agggagcggc
    15001 gttaattcaa cctgtttgat aagcctggag attattgatc aacaatactg cgtcataaga
    15061 aatctctatt agacaaagat ttcagtacct gttggcatat tgcaaaaata acaccaatac
    15121 ggaatcgtca tgttcacgat taaaacagat gatctcaccc atccagcagt gcaagcatta
    15181 gtggcttacc atatttccgg catgctgcag cagtctcccc ccgaaagtag tcatgcttta
    15241 gatgtgcaaa aattacgtaa cccgaaagtg acattctggt cagtatggga aggcgaacaa
    15301 ctcgcaggaa ttggtgcgct gaagttgctg gatgataaac atggcgaact gaaatcaatg
    15361 cgtaccgcgc caaattattt acgtcggggt gtcgccagtc tgattttacg ccacattttg
    15421 caggtcgccc atgacagatg ccttcatcgc ctgagcttag aaacgggtac acaggctgga
    15481 tttacggcct gccatcaact ttatttgaaa catggattcg ttgattgcga accgtttgct
    15541 gattatcaac ttgatccgca cagtcgattt ttgtcattga cgctatgcga aaataatgaa
    15601 ttgccatgag ccagacgcag cacattcttg cattcgacgt gctgcgcctt tatttattat
    15661 cagccgcaaa tgtcttgtcc ttagacgctc tttccacatg cgccacaaga aacctctatt
    15721 ccagtgacac aattatgcct gattatttcc ctagcccgga atatgaataa aaacatcaaa
    15781 taagatgaaa ttatcaaaat attaatgttg cgtctgaggt cgtaacaaca tccgacgtgg
    15841 tgatatgtca aatgagacac tctgatggtc aaagggttta tcgcgtttac agatcgtgga
    15901 tggcagaaaa ctttcagaat caggtttcca ttttaaacca gaaattaagt gagtttgtcc
    15961 catccatgcc ccacgcggtg agatcggatg taatcaacaa caggttacaa aacctacacc
    16021 tgcatgctca tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt
    16081 tgcattgaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttaacgc cacgccgggt
    16141 tcaccgagag tgaatttttc tgcctgcgtg cgggcggctg tttgtgtatc gcttatgcga
    16201 tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgtggcag tgattcctgt
    16261 gtacgcgcac tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc
    16321 gctgacattt tcatctcccg gataatttct ggcagcaaag cctaccagta agtcaataag
    16381 acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg cggatagaaa aaaacgaaag
    16441 cacaaataat gggagcgtca atttttcgag tttgctgacc cgggagtgag tcttgttcca
    16501 ctttgccaat aacgccgtcc ataatcagcc acgaggtgcg cgatgaatgc gactgcagcc
    16561 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
    16621 gttgccggct ccgcagcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc
    16681 gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa
    16741 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaagttccg
    16801 gcagataaag ttcacgaact gcgcctgcgc ctggcgcaac aaggtttgcc caaaggcggc
    16861 gcggtcggtt tcgaactgct cgatcaggaa aagtttggta tcagccagtt cagcgaacag
    16921 gtgaattatc agcgggcgct ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg
    16981 gtaaaagggg cgcgcgtaca tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa
    17041 aaatcccctt ctgcatcggt gacggtaaat ctgttacccg gccgcgcact cgatgaaggg
    17101 caaattagcg ccattgtaca tctggtttcc agcgcggttg ctggtctgcc gccggggaac
    17161 gtcacgctgg tggatcaggg tggacatctg ttaacccagt ccaataccag cgggcgcgat
    17221 cttaatgacg ctcagttgaa atatgccagc gatgtcgaag gccggattca gcggcgtatt
    17281 gaagcgatcc tgtcgccaat tgttggtaac ggtaatattc acgcccaggt cacggcgcaa
    17341 ctggacttcg ccagtaaaga acaaacagaa gaacagtatc gccctaacgg tgatgaatct
    17401 caggcggcgc ttcgttcacg ccagcttaat gagagcgagc aaagcggttc cggttatcca
    17461 ggcggcgtac cgggggcgtt gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc
    17521 acgcctccgg caaatcaaaa taaccgccag cagcaggcga gcaccaccag caatagtggg
    17581 ccgcgtagca cacagcgtaa tgaaaccagt aactacgaag tcgatcgcac cattcgtcat
    17641 accaaaatga acgtgggcga tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa
    17701 accttgccag acggtaaacc gttgcctctc agcaacgaac agatgaagca aattgaagat
    17761 ctgacccgcg aggcgatggg cttttctgaa aaacgcggtg actcgctcaa tgtcgttaac
    17821 tcgccgttca atagtagtga ggaaagcggc ggagaactgc cgttctggca acaacaagcg
    17881 tttatcgatc agttacttgc tgccggtcgc tggttgctgg tactgctggt ggcgtggctg
    17941 ctgtggcgta aagcggtacg cccgcagcta acacgtcgcg ctgaggcgat gaaagctgta
    18001 cagcaacagg cgcaggcccg cgaggaagtg gaagatgcgg tggaagtccg cctgagcaaa
    18061 gacgaacaac tccaacaacg gcgcgctaac caacgtctgg gggcagaagt catgagccag
    18121 cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg
    18181 ataaataacg atcatgagta acctgacagg caccgataaa agcgtcatcc tgctgatgac
    18241 cattggcgaa gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg aagtgcaaac
    18301 tctgagcgct gcaatggcga acgtcacgca gatctccaac aagcagctaa ccgatgtgct
    18361 ggcggagttt gagcaagaag ctgaacagtt tgccgcactg aatatcaacg ccaacgatta
    18421 tctgcgttcg gtattggtca aagctctggg tgaagaacgt gccgccagcc tgctggagga
    18481 tattctcgaa acccgcgata ccgccagcgg tattgaaacg ctcaacttta tggagccgca
    18541 gagcgccgcc gatctgattc gcgatgagca tccgcaaatt atcgccacca ttctggtgca
    18601 tctgaagcgc gcccaggccg ccgatattct ggcgttgttc gatgaacgtc tgcgccacga
    18661 cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg cggaactgac
    18721 cgaagtactg aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt
    18781 gagaacggca gccgaaatta tcaacctgat gaaaacgcag caggaagaag ccgttattac
    18841 cgccgtgcgt gaattcgacg gcgagctggc gcagaaaatc atcgatgaga tgttcctgtt
    18901 cgagaatctg gtggatgtcg atgatcgcag cattcagcgt ctgttgcagg aagtggattc
    18961 cgaatcgctg ctgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga aattcctgcg
    19021 caatatgtcg cagcgtgccg ccgatattct gcgcgacgat ctcgccaacc gtggtccggt
    19081 gcgtctgtcg caggtggaaa acgaacagaa agcgattctg ctgattgtgc gccgccttgc
    19141 cgaaaccggc gagatggtga ttggcagcgg cgaggatacc tatgtctgat aatctgccgt
    19201 ggaaaacctg gacgccggac gatctcgcgc caccacaggc agagtttgtg ccaatggtcg
    19261 agtcggaaga aaccattatt gaagaggccg aacccagcct tgagcagcaa ctggcgcaac
    19321 tgcaaatgca ggcccatgag caaggttatc aggcgggtgt tgccgaaggt cgccagcaag
    19381 gtcataagca gggctatcag gaaggactgg cccaggggct ggaacaaggt ctggcagagg
    19441 cgaagtctca acaagcgcca attcatgccc ggatgcagca actggtcagc gaatttcaaa
    19501 ctacccttga tgcgcttgat agtgtgattg cttcgcgcct gatgcagatg gcgctggagg
    19561 cggcacgtca ggtgatcggt cagacgccaa cggtggataa ctcggcactg atcaaacaga
    19621 tccaacagtt gttgcagcaa gaaccgttat tcagcggtaa accacagctg cgcgtgcacc
    19681 cggatgatct gcaacgtgtt gatgatatgc tcggcgcaac tttaagtttg catggctggc
    19741 gcttgcgggg cgatcccacc ctccatcctg gcggctgtaa agtctccgcc gatgaaggcg
    19801 atctcgacgc cagtgtcgcc actcgctggc aagaactctg ccgtctggca gcaccaggag
    19861 tggtgtaatg accacgcgcc tgactcgctg gctaaccacg ctggataact ttgaagccaa
    19921 aatggcgcag ttgcctgcgg tacgtcgcta cgggcgatta acccgcgcta ccgggctggt
    19981 gctggaagcc accggattac aattgccgct cggcgcaacc tgtgtcattg agcgccagaa
    20041 cggcagcgaa acgcacgaag tagaaagcga agtcgttggc tttaacggtc aacggctgtt
    20101 tttaatgccg ctggaggaag tcgaaggtgt cctgcccggc gcgcgtgttt atgccaaaaa
    20161 catttcggct gaagggctgc aaagcggcaa gcagttgccg ctcggttcgg cgttattagg
    20221 tcgcgttctg gacggcagcg gtaaaccgct tgatggcctc ccctcccccg atacgacgga
    20281 aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa cgtacaccga ttgaacatgt
    20341 gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc gtcgggcgcg ggcagcgtat
    20401 ggggctgttt gccgggtccg gcgttggtaa aagtgtgctg ctggggatga tggcacgtta
    20461 cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa cgagggcgcg aagtaaaaga
    20521 ttttattgag aatatcctcg gtgccgaagg acgtgcacgc tccgtggtga ttgccgctcc
    20581 ggcggatgtt tctccgctcc tgcgaatgca gggtgccgcc tatgccacgc gcattgccga
    20641 agattttcgc gatcgtggtc agcatgtgtt gctgattatg gactccctca cccgctacgc
    20701 gatggcccag cgtgaaattg cgctggcgat tggcgaacca cctgccacta aaggttatcc
    20761 accgtcggtg tttgccaaat taccggcact ggtcgagcgt gccggaaatg gcattagcgg
    20821 cggcggctcg attaccgcgt tttataccgt gctcaccgaa ggcgatgacc agcaggaccc
    20881 cattgccgac tccgcgcggg caatcctcga cggtcacatt gtgctgtctc gccgactggc
    20941 ggaagccggg cactatccgg ctatcgatat tgaagcgtcg atcagtcgcg caatgacggc
    21001 gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt
    21061 tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct
    21121 cgacaaagcc atcgccctgt ggccgcagct ggagggctat ttgcaacaag gcatttttga
    21181 acgcgcggac tgggaagcgt ctctccaggg gctggagcgt attttcccga cagtatcata
    21241 acccaggaga taacggcaga tgacagaaca tggcgcgctg gcgaccctga aagatctggc
    21301 agaaaaagag gtagaggatg ccgcgcgcct gctgggtgaa atgcgtcggg gatgtcagca
    21361 ggcggaagaa cagctcaaaa tgctgatcga ttatcagaat gaatatcgca ataacctcaa
    21421 tagcgatatg agtgccggga tgaccagcaa ccgctggatc aactatcagc agtttatcca
    21481 gacgctggaa aaagccatta ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt
    21541 agacattgcc ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggctt ggcagacact
    21601 gcaggaacgg caatccacgg cggcactgct tgcagaaaac cgcctcgatc agaaaaagat
    21661 ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa tgattcgcat agcgcccttg
    21721 attaccgctg acgttgataa cgccacattg cctggcggca aagccagcga tgctgcacaa
    21781 gattttctcg cgttgttgag cgaagcatta gcaggcgaga caacaaccga caaagcggcc
    21841 ccccagttgc tggtggcaac agataagccc acgacaaaag gcgagccgct ggtcagcgat
    21901 attgtttccg atgcgcaaca agcagattta ctgatccctg tggatgaaac accgcctgtc
    21961 atcaacgaca aacaatccac atcaacatcg ttaaccaccg ctcagacgat aacgttggct
    22021 gcggcggctg acaacaatac ggcaaaagac gaaaaagcgg atgatctgaa tgaagacgtc
    22081 accgcaagcc tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg
    22141 actgatgcac cgtcaaccgt gttaccgaca gagaaaccaa cgcttttcac aaaactgact
    22201 tctgcgcaac tcactacagc acagcctgat gacgcccccg gcacaccagc tcagccatta
    22261 acaccgctgg tagcagaagc ccagagtaaa gcggaagtca tcagcacgcc ttcgtcggtg
    22321 accgctgccg ccagcccgct aatcactcca caccagacac agccactgcc caccgtcgcc
    22381 gcgcctgttt tgagtgcacc gctgggttct cacgaatggc aacaatcatt aagccagcat
    22441 atttcgctgt tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca cccacaggat
    22501 ttaggtgaag tgcaaatttc cctcaaagtg gatgataacc aggcgcaaat ccagatggtt
    22561 tcaccgcatc agcacgtacg cgccgccctg gaagcagcgc tgccggtact gcgtacgcag
    22621 ctggccgaaa gtggcattca gttagggcaa agcaacatca gtggcgaaag ctttagtggt
    22681 cagcagcagg ccgcttccca acaacagcaa agccaacgca cagcaaacca tgaacctctg
    22741 gcgggggaag acgacgatac gcttccggtt cccgtctctt tacaagggcg tgtaacaggc
    22801 aacagcggcg ttgatatttt cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc
    22861 cccgctttgt tgcgctcaag acgcaggata attagccgat aagcagtagc gacacaggaa
    22921 gaccgcaaca catgactgat tacgcgataa gcaagaaaag caagcgatcg ctttggatcc
    22981 cgattctggt attcattacc ctcgcggcct gtgccagcgc aggttacagc tactggcatt
    23041 cgcatcaggt tgccgctgaa gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct
    23101 atgcgctgga tacctttacg gtcaatctgg gcgatgcgga tcgcgtactt tatatcggca
    23161 taaccctgcg actcaaagat gaagctaccc gctcgcggct gagtgagtat ttgccggaag
    23221 tccgtagccg tttgctgtta ctgttttcgc gtcaggatgc tgccgtactg gcgacagaag
    23281 aaggtaagaa aaacctgatt gccgagatta aaaccacact ttccaccccg cttgttgccg
    23341 ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc ttttattctg cgataacgac
    23401 atgggcgata gtattctttc tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa
    23461 gtcaaagacg aaccgacagc cagtgttagc ggcgaaagtg acattcgtcc gtacgatccg
    23521 aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc
    23581 tttgcccgcc attttcgtat ggggctgttc aacctgctgc gccgtagccc ggacattacc
    23641 gtcggggcca tccgcattca gccgtaccat gaatttgccc gcaacttgcc ggtgccgacc
    23701 aacctgaacc ttatccacct taaaccgctg cgcggcacag ggctggtggt gttctcaccg
    23761 agtctggtgt ttatcgccgt ggataacctg tttggcggcg atggacgctt cccgaccaaa
    23821 gtggaaggtc gcgagtttac ccatacagaa cagcgcgtca tcaaccgcat gttaaagctg
    23881 gcgcttgaag gctatagcga cgcctggaag gcgattaatc cgctggaagt tgagtacgta
    23941 cgttcggaaa tgcaggtgaa atttaccaat atcaccacct caccgaacga catcgtggtt
    24001 aacacgccgt tccatgtgga gattggcaac ctgaccggcg aatttaatat ctgtctgcca
    24061 ttcagcatga tcgagccgct acgggaattg ttggttaacc cgccgctgga aaactcgcgt
    24121 aatgaagatc agaactggcg cgataacctg gtgcgccagg tgcagcattc acagctggag
    24181 ctggtcgcca actttgccga tatatcgcta cgcctgtcgc agattttaaa actgaaaccc
    24241 ggtgacgtcc tgccgataga aaaacccgac cgcatcatcg cccatgttga cggtgtcccg
    24301 gtgctgacca gtcagtacgg caccctcaac ggtcagtatg cgttacggat agaacatttg
    24361 attaacccga ttttaaattc tctgaacgag gaacagccca aatgagtgac atgaataatc
    24421 cggccgatga caacaacggc gcaatggacg atctgtgggc tgaagcgttg agcgaacaaa
    24481 aatcaaccag cggcaaaagc gctgccgacg cggtgttcca gcaatttggc ggtggtgatg
    24541 tcagcggaac gttgcaggat atcgacctga ttatggatat tccggtcaag ctgaccgtcg
    24601 agctgggccg cacacgaatg accatcaaag agttattgcg tctgacgcaa gggtccgtcg
    24661 tggcgctgga cggcctggcg ggcgaaccgc tggatattct gatcaacggt tatttaattg
    24721 ctcagggcga agttgtcgta gtcgctgata aatatggcgt gcggatcacc gatatcatta
    24781 ctccgtctga gcgaatgcgt cgcctgagcc gttagtgatg aataaccatg ctactgtgca
    24841 atcttccgcg ccggtttcta ctgcgccact gctgcaggtg agcggcgcac tcatcgccat
    24901 tattgccctg atcctcgctg ctgcctggct ggtaaaacgg ctgggatttg ccccgaaacg
    24961 cactggcgtt aacggtctga aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt
    25021 cgtggtggtc gatgtggaag atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa
    25081 tctgctgcat aaacttcccc cttcagcacc aacggaagag ataccgcaga ccgattttca
    25141 gtcggtcatg aaaaacttgc ttaagcgtag cgggagatcc tgatgcgtcg tttgttgtct
    25201 gtcgcacctg tccttctctg gctggttaca cccctcgcct tcgcgcaact gacgggtatt
    25261 accagccagc cgctgcctgg cggtggacaa agctggtcgc tcccggtgca gacgctggtg
    25321 ttcattacct cgttgacgtt tattccggca attttactga tgatgaccag tttcacccgc
    25381 atcatcattg tttttggttt attgcgtaac gcactgggaa caccctccgc gccacctaac
    25441 caggtattgc tggggctggc actgtttttg acctttttta ttatgtcacc ggtgatcgat
    25501 aaaatttatg tcgatgcgta ccagccattc agcgaagaga aaatctcaat gcaggaggcg
    25561 ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc gtcagacccg tgaggcggat
    25621 ttaggattgt ttgccagact ggcaaatact ggcccgttgc agggacctga agccgtgccg
    25681 atgcgcattt tgctcccggc ttacgtgacc agcgagttga aaaccgcatt tcagataggc
    25741 ttcacgatct tcattccgtt tttgattatc gacctggtga ttgccagcgt gttgatggca
    25801 ttggggatga tgatggtccc ccccgccacc attgctctgc cctttaaact gatgctgttt
    25861 gtactggtgg atggctggca attgctggtc ggttcgctgg cgcagagctt ttacagctag
    25921 agaggcaaaa tgacacctga atcggtcatg atgatgggga ctgaagcgat gaaagtcgcg
    25981 ctggcactgg ctgccccgct attgctggta gcattggtca cgggccttat catcagtatt
    26041 ttgcaggccg ccacgcagat taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc
    26101 gtatttatcg ccattattat tgccggaccg tggatgctca atctgttgct ggattacgtc
    26161 cgcaccttgt tcactaacct gccgtatatc atcgggtagg ccgtacaatg atgcaggtga
    26221 caagcgatca atggctttcc tggctaagtc tgtacttctg gccgttactg cgcgtgttgg
    26281 cgctgatctc caccgcgccg attctgagcg aacgcagcgt accgaaacgg gtaaaactgg
    26341 gtctggcaat gatgatcacg ttcgctattg ccccatcatt acctgccaac gatgttcctg
    26401 ttttttcgtt ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg
    26461 gttttaccat gcaatttgcc tttgccgcgg tgcgaaccgc tggtgaaatt atcggtctgc
    26521 aaatggggct gtcatttgcg acgtttgtcg atccggccag ccatcttaat atgcccgttt
    26581 tagcgcgtat catggatatg ctggcgttac tgctgttcct gacatttaac ggtcatttat
    26641 ggttgatttc actgctggtc gatacctttc acaccctgcc aattggtggc gaaccgttga
    26701 acagcaatgc gtttctggca ctcaccaaag cagggagttt gattttcctt aacgggctga
    26761 tgctggcgtt accgctcatt actctgctgc tgaccctgaa tctggcatta ggtttactaa
    26821 atcgtatggc cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg
    26881 gtatctcttt aatggcggca ttaatgccgt taattgcgcc tttttgcgaa catttattca
    26941 gtgagatgtt taatttgctg gctgatatta ttagtgaatt gccattaata taattccgta
    27001 acgtttatca tgctacccta aggattatcc gaaaaataat acctacgaac atcttccagg
    27061 atactcctgc ggcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttac
    27121 tttaagacaa ttccaggcaa attatacaac actttacggg atagtaagtc cgcctgaaaa
    27181 atcgcgagag tggcgcatta ggtgacccat gttgttccgt ttagtcatga tgaaatattc
    27241 aggtaagggg aattatcgtt acgcattgag tgagggtatg ccatgtcaac gattattatg
    27301 gatttatgta gttacacccg actaggttta accgggtatc tgttgagtag aggggttaaa
    27361 aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg ccatagcttg tgattcacag
    27421 cgcccttcag tggtgtttat taatgaggac tgtttcatcc acgatgcttc taacagtcag
    27481 cgcatcaagc acatcattaa tcaacatccc aatacgttat ttatcgtttt tatggcaatt
    27541 gccaatgttc attttgatga atatctattg gtcagaaaaa atttattgat cagttctaaa
    27601 tcgattaaac cggaatctct cgatgatatc cttggcgata ttctgaaaaa agagacaacg
    27661 ataacctcgt ttttaaatat gccgacgtta tcattgagcc gaaccgaatc gagtatgttg
    27721 cgaatgtgga tggcaggtca gggaaccatt caaatctccg accaaatgaa tatcaaagcc
    27781 aagaccgttt catcgcataa aggtaatatt aaacgtaaga tcaaaacgca taataaacag
    27841 gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt tgtcaacatg
    27901 cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaattagat tactctgctt
    27961 tctccacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg ccgagcgggt
    28021 agtcttccag cgaaaccagg tacattgtgc cttcactaaa ctcctcaact gccagtacca
    28081 cgccaggacg acgcggaccg ccatccgttt tgactgttac ccgatcattc accttcatcg
    28141 ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc agcgccaggc
    28201 gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc ggacttaacg
    28261 actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg caaaagagta
    28321 cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa
    28381 tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg ttagtgtcga
    28441 tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg atattcatga
    28501 cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaaatcccg accctgaggg
    28561 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    28621 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgtgatatg
    28681 aatgattaac gctacttatg aatagaaatg tgacgcgcgt caccccttgg tggctctggc
    28741 gtcaacggga tcgctcccaa aaaaatcatc tttgataatt tgtgctgttt tgagaatcat
    28801 cctggtaagg ggagtactgc gcaaccatgc tttcaattca acaaccacta ctggttttta
    28861 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg gcagccccct
    28921 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    28981 cggaaatgct gtacttgcaa aaaatgttgg ggctacaagg cttaccgctg attgcagaga
    29041 atggcgcagt gatccagctt gctaagcaat ggcaggagat agacggtttt ccacgcatca
    29101 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    29161 attttaaatt cacgactttt gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    29221 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    29281 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttgcagt
    29341 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggctg
    29401 ccaactggat tatcgcgacc tatcaacaat tgtcaggcaa acgcccaacc acacttggcc
    29461 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    29521 aagggctaaa ccgtgaaggg gtccatctgc atgatgagga tccggcccgc gtctggcgaa
    29581 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgcccgtt
    29641 aagcgttatc gctcgcgaat acccgattgc gcccggcctg ttttgcgagg taaagccgac
    29701 ggtcggccaa tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctgctta
    29761 cccccagcga ggcactgatg cgtatcgtcg tactcttggc gatcagcatc tctttttcat
    29821 ttaacttcag gcgaatacgt tctgcgacct ccgcagcctg cgtcagattc gcgcctggca
    29881 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgtaaggaac
    29941 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cacctgatga ccaaagcggt
    30001 cattaatcgc tttaaaatgg tcaagatcga cctgaatgac agaaaatgga tgttggtgcg
    30061 tctgacacaa tttagcgagc ggacgggctt tttcgaacag tgcgccacgg ttatataaac
    30121 gcgttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    30181 tgaccatccg gcgaatcaca taccaggaga gtagtaacat ggtggtaaag agcgcccaca
    30241 gcagggttaa tgcaatgctg atactgccga aatcaccacg cacgccttcg cttagcgtat
    30301 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca taacgactgt
    30361 tcatacgaat gccaccccgc gtgtcatgtt ccattgcctg cgccaacaag gccagttcac
    30421 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    30481 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    30541 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttttcactgt
    30601 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    30661 tgaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    30721 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    30781 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggagttgt
    30841 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    30901 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgacacg ccattgactg
    30961 gcagggtgcg tcgtcggttg agttcgattt gccaggcgtg ctcgtcgcga tgctgttcga
    31021 actcggttac ggcattacgc agagaggtga aatccagtgg cgctacgaga gcttcgcgca
    31081 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    31141 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcaccacca
    31201 cttcacgcca ggtgagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    31261 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    31321 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacccggc
    31381 gataacgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    31441 attcattgca ggcattggca caccaggccg gatgtgatcc ggcctggtgt taatgatacc
    31501 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagatcgg tttcatgaaa
    31561 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    31621 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    31681 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    31741 tttgcataat agccagtgcg agtattaatg tgcctgaccg cgcattttcg ctactgcttt
    31801 cactcccagc accacgatgc cgccgatgat aaaaccaaga atcagattta aaacagtcgg
    31861 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    31921 cggcgcaata ccatgtacca caatcccgcc gccgacgagg aacatcgcca gcgtgccgac
    31981 aatcgataat gctttcatca gccagggcgc gataatcaat aaccctttac ctaatgcctg
    32041 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    32101 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    32161 ctgattaagc aacggcgcct ctgccacaat ccccagcgtg atggcaacga tttccgcaga
    32221 caaaataaaa tcggtacgaa tcgccccttt aattttgtcc ttttcaaact tcagcggatc
    32281 ctgcgccgcc agcttctcca gacgctgctg actctgcgcc ggatcttctt tatgtttacg
    32341 cgcctccagc atatgcagca ctttctctac cccttcaaag cagagaaacg cgccgccaat
    32401 catcaacagt ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    32461 cagaatcact ttattgatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    32521 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    32581 ggcggttttc ttcgccgcca gtttgcccat cacggaaata tcgtccagca gtgtggcgat
    32641 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    32701 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    32761 tttttttaac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    32821 atgacccacc tttttatttc agctgtgagg gattatgcgt ttccgccagt tgttaccgct
    32881 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    32941 tggcgtggaa agctggccgc cgttaatgat ggcgggcgtg cgattcctgg cagccggtat
    33001 tttattgctg gcatttttgc tactgcgcgg acacaaactt cccccgctac gtccgctgct
    33061 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    33121 cgaacatcaa aatgttcctt ccggcatcgc cgccgtagtg gttgcaaccg tgcccctctt
    33181 taccctgtgc ttcagccgcc tgtttggcat taaaacgcgc aaactggaat gggtgggtat
    33241 cgccattggg cttgccggaa tcatcatgct caatagcggt ggaaatttaa gcggcaatcc
    33301 gtggggcgcg attctgattt taatcggctc gattagctgg gcgtttggct cggtttatgg
    33361 ctcgcgcatt accttacctg tagggatgat ggcgggtgcg attgagatgc ttgcggcagg
    33421 cgtggtgtta atgattgcgt cgatgattgc gggtgaaaaa ctgacggcgc tcccttccct
    33481 ttcaggcttc cttgcggtcg gctatctggc gctgtttggt tcgattatcg ccattaacgc
    33541 ttatatgtat ttaatccgca acgtcagtcc ggctctcgcc accagctacg cctacgttaa
    33601 cccggtggtc gcggtcttac tgggtacggg actgggtggc gaaacgctgt cgaagattga
    33661 atggctggcg ctcggcgtaa ttgttttcgc ggtggtactg gtcacgttgg gaaaatatct
    33721 cttcccggcc aagcccgttg tcgcgccagt tattcaggat gcatcaagcg agtaaatgaa
    33781 tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca gatccactct tccagacgct
    33841 cggtaagcgc cgcatccgta agcttctcgc gcccacgtaa cgcacactcc cagacaatca
    33901 gtacgcgcca gccgagttcc tgcaagcgac tgatatcgcg gcgatcgcgt tcaacatttt
    33961 tacctatctt ctccagccag aactcggttc gagtcgcagg cactttaaac agatagcagt
    34021 gatgatgatg ccagaagcag ccgtgggtaa atatcacgca gcgatattca tcaacgacaa
    34081 aatccgggcg tccgggcaga ctggcgtcct gaacgcgaaa cgcaaggccc tgcccggtta
    34141 acaaactggc gaggcgcttc tcaatcgccg tatcacgcgt ggcaatcgcg cgcatatttt
    34201 tgctgcgggt ggccttatcg tgaacgtcga ccatgttgtg cctcttgctg acgcaacgcc
    34261 accgcctgtt tgatttttgg ctcaagcagt tttgccaccg cggcaaagac cggcacgacc
    34321 accgagttac cgaactggcg ataggcctga gtgtccgaaa ccggaatgcg gaatttcact
    34381 tctcctggcg cttcaaaacc cattaagcgc gcacattccc gaggcgttaa ccgacgtggg
    34441 cgatgttgct gattcagcgg atcgtcaaag tctttttcac ccgtggccat atcccagccg
    34501 cgatcgatta aaatttccgc gccatctttg taataacgag cagacagcgt acgcgtgacg
    34561 ctttgcggat tgttcggata aaccattcca taaccgaagc cattaccgcg cgcctgatgt
    34621 tttttcgcat aacgatagag gtacttccac agcaccggcg tcaggatata tttcgcctcg
    34681 accatcgggt ccagcagctg ggccagcgtc actcgctgcg cagggaaaca ttcgctgata
    34741 tcacgcaggg taaaatcggc tttcagattc agatcgcgac gaaaacccac cagcacgatg
    34801 cgttcacggt gctgcggcag gaaatgtttg ccatcgatga ttttcggatc gtccggcccg
    34861 ttatcttctg catcagccac gtcatagccc agttcgtcca gcgtctgcat gatgatgcgg
    34921 aacgttttac cctggtcgtg acttttcagg tttttgacgt tttcgagcac aaacatcgcc
    34981 ggacgacgcg cgtcgataat gcgcaccaca tcaaagaaca gcgtgccctg agtatcgcag
    35041 gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata cgccagccag cgaaaatggc
    35101 tgacaaggaa aacccgccag taatacatcg tgttcaggaa tgtgttgacg aatatgttcc
    35161 gccgccgcct catcactcac gccttcttta tggctgaggg tgatgtcgcg gatatcttca
    35221 ttaaaatgat gcgtcgccgg gtcgcaataa tgattggctt tataagtgcg taccgcatgt
    35281 ttgttccatt cgctggtaaa cacgcactgt ccgccaatcg attcaaaacc gcgacggatg
    35341 ccgccaattc cggcaaatag atcgataaag cgaaacgcat aatgcggatg atgtgccggt
    35401 ggtttgggta acaacgtttg cagatgggcg aactctttct cacttaaacg gtgccatgcc
    35461 gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc agtgattctc acccacacca
    35521 ttaagctgcg ccaccagcgt tttaacatca taaatttgca gcagtttctc cagcattgcc
    35581 tgaacgacat tcccggtgct gtatgaatcg gttactgata tattttcctg catagattta
    35641 accggccatc tgagatggcc gacaggttaa cataattaca gccatttagg gccacgcgct
    35701 cagcccgcgg gtgaaaacgc atctatcacc ttgtgatcga caccttcatt ctcccccgcc
    35761 agttcggcac tgagcttcgc cataaactcg actaaaaatt gcgcgttgtg ctgcgccagc
    35821 tgcttgcctc ttgcggtttg catggtttgc ggtagcttca gcaacttagt ctggaaatga
    35881 tccagcgcgt agcgtttatc atccagcggg cgatgctgtg cgaacggatc ttcaccatca
    35941 aacagcgcca cgcccaatgc ccctgaaaca gcaaacacgc gcgccaggcc aatcgccccc
    36001 agggcttcca gccgatccgc atcctggacg attttagctt ccgttgttaa gggggcgatt
    36061 tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg cctcgatttt ctccgccgga
    36121 aattgcacga actcttcacg gagcaggcga cgcgcctctt ctgccgccag gattgaagaa
    36181 tgctgccgtt gcggatgatt tttcgccagg ctcacaatat cgtgaaaata acaggcggtt
    36241 aaaatcacca gcatatcgac atcgtcatct gccgccagct tctgtgccgt agcccagacg
    36301 cggcgaaaat gacatacgtc atgggccgcg tcctggtgct gatgatggtt tttcaaccag
    36361 ttttcgaact gtgcctgcca gtgttgtaag tccatacgcg ctccgttgcc aaagagtcgc
    36421 aaccttagca gtttttattc atagcgtctg ctacgtgcgg gacgaggtcg ggtgtaccag
    36481 gcaatgctgc ctaacaatgc cccgaccaga gccataaata aaaatccgac cagtgcccct
    36541 aaccactttc ccgcggtaga gcctaaatcg ctttcaaatc ccgacaccgg ggcatcaggg
    36601 atccggctca tcacccagat gtaacgcgcc attgcccaga cgaaatagcc acaccatgta
    36661 tagaaagccc acaaagccaa tttgccgcca gggctgcgag agagtctctc ttccatcttt
    36721 gtcgaaccgt attccatttt gagataaccc ggaaaattca gacttagtac tattatgtga
    36781 taaaagtcac atttttccac attggatgag ccagtcaaga ctcaatcatt ttttgaacaa
    36841 atagataatt tttgcgcaac ggtagtgaat acaggaaaaa tcgttttgag cgattttatt
    36901 tatctttgat atcggcttta ttaattcatt caatcaatat attagcactg attacaatta
    36961 taccttttta ttattctgtg tcatgatgcc tccgttatta gccttttatc gtcttgttta
    37021 tattttttgg gccggcatga tgccggcttt tttttattcc ttcattaatg tgcgcgtgat
    37081 cacaccagcc gtttggcgca acaatcattg atacccccta tgtttccgct caactcataa
    37141 catattgata tgcattcata atattcgtga atatttatat tcagaattca taattatgaa
    37201 ttatattaaa tagattagaa acatt
//
LOCUS       DAZGTJ010000043        35921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000043 DAZGTJ010000000
VERSION     DAZGTJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.043"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            78..362
                     /gene="pduA"
                     /locus_tag="ACHK8E_002666"
     CDS             78..362
                     /gene="pduA"
                     /locus_tag="ACHK8E_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFP0344913.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            359..1171
                     /gene="pduB"
                     /locus_tag="ACHK8E_002667"
     CDS             359..1171
                     /gene="pduB"
                     /locus_tag="ACHK8E_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFP0344914.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1190..2854
                     /gene="pduC"
                     /locus_tag="ACHK8E_002668"
     CDS             1190..2854
                     /gene="pduC"
                     /locus_tag="ACHK8E_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFP0344915.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2864..3532
                     /locus_tag="ACHK8E_002669"
     CDS             2864..3532
                     /locus_tag="ACHK8E_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFP0344916.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3547..4065
                     /gene="pduE"
                     /locus_tag="ACHK8E_002670"
     CDS             3547..4065
                     /gene="pduE"
                     /locus_tag="ACHK8E_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFP0344917.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4075..5907
                     /locus_tag="ACHK8E_002671"
     CDS             4075..5907
                     /locus_tag="ACHK8E_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFP0344918.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            5897..6247
                     /locus_tag="ACHK8E_002672"
     CDS             5897..6247
                     /locus_tag="ACHK8E_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFP0344919.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6267..6542
                     /gene="pduJ"
                     /locus_tag="ACHK8E_002673"
     CDS             6267..6542
                     /gene="pduJ"
                     /locus_tag="ACHK8E_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFP0344920.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6566..6988
                     /locus_tag="ACHK8E_002674"
     CDS             6566..6988
                     /locus_tag="ACHK8E_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFP0344921.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6991..7623
                     /locus_tag="ACHK8E_002675"
     CDS             6991..7623
                     /locus_tag="ACHK8E_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFP0344922.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            7620..8111
                     /gene="pduM"
                     /locus_tag="ACHK8E_002676"
     CDS             7620..8111
                     /gene="pduM"
                     /locus_tag="ACHK8E_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFP0344923.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8115..8390
                     /locus_tag="ACHK8E_002677"
     CDS             8115..8390
                     /locus_tag="ACHK8E_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFP0344924.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8402..9409
                     /gene="pduO"
                     /locus_tag="ACHK8E_002678"
     CDS             8402..9409
                     /gene="pduO"
                     /locus_tag="ACHK8E_002678"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFP0344925.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9406..10791
                     /gene="pduP"
                     /locus_tag="ACHK8E_002679"
     CDS             9406..10791
                     /gene="pduP"
                     /locus_tag="ACHK8E_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFP0344926.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10802..11914
                     /locus_tag="ACHK8E_002680"
     CDS             10802..11914
                     /locus_tag="ACHK8E_002680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFP0344927.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11911..13254
                     /locus_tag="ACHK8E_002681"
     CDS             11911..13254
                     /locus_tag="ACHK8E_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0344928.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13257..13811
                     /gene="pduT"
                     /locus_tag="ACHK8E_002682"
     CDS             13257..13811
                     /gene="pduT"
                     /locus_tag="ACHK8E_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFP0344929.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13811..14161
                     /gene="pduU"
                     /locus_tag="ACHK8E_002683"
     CDS             13811..14161
                     /gene="pduU"
                     /locus_tag="ACHK8E_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFP0344930.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14166..14609
                     /locus_tag="ACHK8E_002684"
     CDS             14166..14609
                     /locus_tag="ACHK8E_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP0344931.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14710..15039)
                     /gene="tmaR"
                     /locus_tag="ACHK8E_002685"
     CDS             complement(14710..15039)
                     /gene="tmaR"
                     /locus_tag="ACHK8E_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP0344932.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15211..16269)
                     /gene="yeeA"
                     /locus_tag="ACHK8E_002686"
     CDS             complement(15211..16269)
                     /gene="yeeA"
                     /locus_tag="ACHK8E_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0344933.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16467..16940)
                     /gene="sbmC"
                     /locus_tag="ACHK8E_002687"
     CDS             complement(16467..16940)
                     /gene="sbmC"
                     /locus_tag="ACHK8E_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP0344934.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17059..18225)
                     /gene="dacD"
                     /locus_tag="ACHK8E_002688"
     CDS             complement(17059..18225)
                     /gene="dacD"
                     /locus_tag="ACHK8E_002688"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP0344935.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18434..19861
                     /gene="sbcB"
                     /locus_tag="ACHK8E_002689"
     CDS             18434..19861
                     /gene="sbcB"
                     /locus_tag="ACHK8E_002689"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP0344936.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19935..21293)
                     /gene="plaP"
                     /locus_tag="ACHK8E_002690"
     CDS             complement(19935..21293)
                     /gene="plaP"
                     /locus_tag="ACHK8E_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP0344937.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21283..21345)
                     /gene="yoeI"
                     /locus_tag="ACHK8E_002691"
     CDS             complement(21283..21345)
                     /gene="yoeI"
                     /locus_tag="ACHK8E_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP0344938.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21560..22489)
                     /gene="yeeY"
                     /locus_tag="ACHK8E_002692"
     CDS             complement(21560..22489)
                     /gene="yeeY"
                     /locus_tag="ACHK8E_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0344939.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22535..23359)
                     /gene="yeeZ"
                     /locus_tag="ACHK8E_002693"
     CDS             complement(22535..23359)
                     /gene="yeeZ"
                     /locus_tag="ACHK8E_002693"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0344940.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23442..23696)
                     /gene="yoeB"
                     /locus_tag="ACHK8E_002694"
     CDS             complement(23442..23696)
                     /gene="yoeB"
                     /locus_tag="ACHK8E_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP0344941.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23693..23944)
                     /gene="yefM"
                     /locus_tag="ACHK8E_002695"
     CDS             complement(23693..23944)
                     /gene="yefM"
                     /locus_tag="ACHK8E_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP0344942.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24227..24277
                     /gene="hisL"
                     /locus_tag="ACHK8E_002696"
     CDS             24227..24277
                     /gene="hisL"
                     /locus_tag="ACHK8E_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP0344943.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    24253..24376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            24423..25322
                     /gene="hisG"
                     /locus_tag="ACHK8E_002697"
     CDS             24423..25322
                     /gene="hisG"
                     /locus_tag="ACHK8E_002697"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP0344944.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25328..26632
                     /gene="hisD"
                     /locus_tag="ACHK8E_002698"
     CDS             25328..26632
                     /gene="hisD"
                     /locus_tag="ACHK8E_002698"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP0344945.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26629..27699
                     /gene="hisC"
                     /locus_tag="ACHK8E_002699"
     CDS             26629..27699
                     /gene="hisC"
                     /locus_tag="ACHK8E_002699"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP0344946.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            27699..28766
                     /gene="hisB"
                     /locus_tag="ACHK8E_002700"
     CDS             27699..28766
                     /gene="hisB"
                     /locus_tag="ACHK8E_002700"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP0344947.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28766..29356
                     /gene="hisH"
                     /locus_tag="ACHK8E_002701"
     CDS             28766..29356
                     /gene="hisH"
                     /locus_tag="ACHK8E_002701"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP0344948.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29356..30093
                     /gene="hisA"
                     /locus_tag="ACHK8E_002702"
     CDS             29356..30093
                     /gene="hisA"
                     /locus_tag="ACHK8E_002702"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP0344949.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30075..30851
                     /gene="hisF"
                     /locus_tag="ACHK8E_002703"
     CDS             30075..30851
                     /gene="hisF"
                     /locus_tag="ACHK8E_002703"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP0344950.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30845..31456
                     /gene="hisIE"
                     /locus_tag="ACHK8E_002704"
     CDS             30845..31456
                     /gene="hisIE"
                     /locus_tag="ACHK8E_002704"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP0344951.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(31551..32528)
                     /gene="wzzB"
                     /locus_tag="ACHK8E_002705"
     CDS             complement(31551..32528)
                     /gene="wzzB"
                     /locus_tag="ACHK8E_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP0344952.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(32675..33841)
                     /gene="ugd"
                     /locus_tag="ACHK8E_002706"
     CDS             complement(32675..33841)
                     /gene="ugd"
                     /locus_tag="ACHK8E_002706"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP0344953.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(34088..35494)
                     /gene="gndA"
                     /locus_tag="ACHK8E_002707"
     CDS             complement(34088..35494)
                     /gene="gndA"
                     /locus_tag="ACHK8E_002707"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP0344954.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         9228 a         9230 c         8980 g         8483 t
ORIGIN      
        1 agccgattaa gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa
       61 atccaggcga ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa
      121 ctgccgccat agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct
      181 acgaaaaaat aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa
      241 aagcggcaac agacgcgggt gccgcagctg cgcgtaacgt cggcgaagta aaagccgtac
      301 acgttattcc ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat
      361 gagcagcaat gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc
      421 agagcatgag gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa
      481 aagctgcacg ttaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat
      541 tgcgaacgtt gatagcgccc tgttggacgc gatgaaactt gaaaaacgct atcgttccat
      601 tggcattctg ggggcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt
      661 aaaagccacc aacactgaag ttattagcat tgaacttcct cgggatacta aaggcggtgc
      721 cggacacggc tcattgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat
      781 tgaagttgcc ctgaaagaac tcgatcggac ctttggtgat gtctatgcca acgaagccgg
      841 gcatcttgaa ctgcaataca ccgcccgcgc cagctatgca ctggaaaaag cgtttggcgc
      901 tccactggga cgggcttgcg gcgttattgt cggcgctccg gctgcggtgg gtgttctgat
      961 ggccgacacc gcgctgaagt ccgccaatgt ggatgtcgtg gcatacagct cccccgctca
     1021 aggcaccagc tacagcaacg aagtcattct gattatctcg ggtgactccg gcgcagtccg
     1081 ccaggcggtc atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac
     1141 accgaaaaac gatcgccctt cctacatctg aattctctga ggcagattta tgagatcgaa
     1201 aagatttgaa gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat
     1261 tgaagaaggc ttcattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt
     1321 taacggcgtc gttaccgaac tggatggaaa acccgccaat cagtttgacc tgatcgacca
     1381 ctttatcgcc cgctacggca tcaatctcgc gcgcgcagaa gaagtcatcg cgatggattc
     1441 ggtaaaactt gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct
     1501 taccacagca atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga
     1561 gatgatgatg gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt
     1621 caccaacatc aaagataacc cggtacaaat tgccgccgac gcagctgaag gcgcatggcg
     1681 cgggtttgat gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat
     1741 cgccctgctg gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga
     1801 agaagccacc gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc
     1861 tgtctatggt acagaacctg ttttcaccga tggcgatgat accccgtggt caaaagggtt
     1921 tctcgcgtca tcctatgcgt ctcgcggctt aaaaatgcgc tttacttccg gctccggctc
     1981 tgaagtacag atgggttacg cggaaggcaa atcaatgctt tatctcgagg ctcgatgcat
     2041 ttacatcacc aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgtatcgg
     2101 tgttccatca gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc
     2161 atcgctggat ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg
     2221 ccgtaccgca cgtttcctga tgcaatttct gccaggtacc gactttatct cttccggttt
     2281 ttccgctgtg ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt
     2341 cgatgattac aacgtcattc agcgtgacct gaaggttgat ggcggcctgc gaccagtacg
     2401 cgaagaagat gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc
     2461 cggtatggga ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg
     2521 ttcaaaagat atgcctgagc gcaatattgt agaagacatc aagttcgccc aggagattat
     2581 caataaaaac cggaacagcc tggaagtggt gaaagccctg gcgcaaggcg gttttaccga
     2641 tgtcgcccag gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac
     2701 ctcagccatc atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta
     2761 cgccggaccg gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat
     2821 tcccggtgca cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa
     2881 attactgcgt cagattatcg aagacgtgct ggcagaaatg caaaccagcg acaagcccgt
     2941 ttcatttcgc gcacctgtct ctgcaactgc ctcttccgcg ccagataccg gaaattttct
     3001 gaccgaaatt ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg
     3061 cccggcgttc ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatttt
     3121 gcgcgaagtg attgccggta tcgaagaaga aggcattaaa gcccgtgtga ttcgctgctt
     3181 taaatcgtcc gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat
     3241 tgctatcggt attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt
     3301 gtctaacctg gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat
     3361 tggcaaaaac gccgcgcgtt atgcaaagcg tgagtctcca caaccggtgc cgaccctcaa
     3421 cgatcaaatg gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac
     3481 gaagtacgtg gtcaccggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa
     3541 accttcatga ataccgatgc aattgagtcg atggtcagag acgtgttgaa ccgcatgaac
     3601 agtttacagg atgcagctcc tgtttctgcg gtccccaacg cgtcaataca gagcgccaaa
     3661 gtgacggatt acccgctggc aaataaacat ccggaatggg ttaaaacggc gaccaataaa
     3721 acgctcgacg atttcactct ggaaaatgtg ctaagcgata acgtcaccgc ccaggatatg
     3781 cgtataaccc ctgaaacatt gcgtattcag gccgcaattg ccagagatgc tggtcgcgat
     3841 cgcctggcga tgaattttga acgcgccgct gagcttacct cagtaccgga tgatcgcatc
     3901 ctcgaaatct acaatgcgtt acgcccttat cgttcgacta agcaagagct tattgccatt
     3961 gctgacgatc ttgaacggcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct
     4021 gcggaacttt atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga
     4081 tatatagcag gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt
     4141 gcaactggag agttgtcgta cgtgagtagc gccctggcgg aaaccaccgg aattaaaggt
     4201 acgttgcgta acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc
     4261 atcaatgtta gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat
     4321 gtggcgatgg aaaccatcac tgaaaccatc atcaccgaat caaccatgat cggtcacaac
     4381 ccgaaaactc cgggcggagt tgggctgggc gtagggctca ccatcactcc gcaagagtta
     4441 ttaacacgcc cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc
     4501 gatatcgcaa cgatgataaa cgcctccgta cgagccggat atcagctgac tggcgttatt
     4561 ctgcaacgcg acgatggcgt actggtcaat aaccgcctgg aaattcctct ccctattgtg
     4621 gatgaagtcc tctacatcga tcgtattccg ctcggcatgc tggctgcaat tgaagttgcc
     4681 gttcccggga aagttatcga aaccctgtct aacccgtatg ggattgccac cgttttcgcc
     4741 ctcaatgctg aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt
     4801 tcagcagtgg tagtgaaaac gccttctggc gacgttaaag cccgttcgat acccgccgga
     4861 aatatcgaac tgctttctgc cgggcgcaca acgcgggttg atgtagctgc tggtgccgat
     4921 gccattatga aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaacctggc
     4981 tccaacatcg gcggaatgct ggaacatgtt cgccagacca tggctgaact gaccaacaaa
     5041 cccagcaacg aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcaac
     5101 gtaaccggtg gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg
     5161 gtgaaatccg accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat
     5221 gttgatgttc aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc
     5281 cctggaacca cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc
     5341 atcatcaatc agaaagggga aatagttgcc actcaccttg cgggagcggg cgacatggta
     5401 acgatgatta tcgctcgtga gctgggctta aatgatcgtt atctcgcaga agaaatcaaa
     5461 aagtacccgt tggcaaaagt agaaagcctg ttccacttac gccatgaaga tggcagcgtt
     5521 cagttcttcc ctactccgct gtcccctcat gtatttgccc gcgtttgtgt ggtgaaacct
     5581 gacgaactgg tgcctattct cggtgatttg acgctggaaa aagtacgtgc tgtccgtcgt
     5641 agtgcgaaag agcgtgtctt cgtaactaat gccctgcgcg cgctacgtca ggtaagccct
     5701 gccggaaata ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc
     5761 gaagtaccgc aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg
     5821 aatatccgtg gtagcgaagg tccgcgcaat gcggtagcta ctgggctcct catcgcctgg
     5881 cacaaggggg cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat
     5941 cggtgactgt atcactacat gggatgaagt gcttcttggc attgaagagg aaggtattcc
     6001 ctttcgcatt caacacattc cgtcaggtga agttatcgat agcgcctggc tggcggctcg
     6061 tcaatctccc ctgttggttg gcattgcctg cgatcaggaa aaactgattg tgcattacaa
     6121 aaacttaccg gcatcagcgc cactgtttac gctgatgtat caacaagata accatgccag
     6181 gcgcagcatc ggcactaacg ctgcccgcct ggtaaaaggg attcccttcc gggaactcca
     6241 ctcataatcc acaggagaaa cgcagtatga acaacgcact aggactggtt gaaacaaaag
     6301 ggttagttgg cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg
     6361 ttggctatga aaagattggt tcgggtctga ttacggtgat ggtccgtggc gatgttggcg
     6421 cagtaaaagc cgccgttgat gcaggaagcg ccgccgcaag cgccgttggc gaagtgaaat
     6481 cctgccatgt catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcagcat
     6541 aaccccattc gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagtcagtgg
     6601 tctggcactc gccatcagtt gcgcagatgc tatggcaaaa gccgcgtcca tcacgctggt
     6661 tggccttgag aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc
     6721 ctcggttaat gcggcgatcg cgacgggagc cagtttcgcc gggcagcgaa atggccttgt
     6781 cgcacacaaa gtaattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt
     6841 gactgtgacg gttgaaacaa gcgaaacacc agcaacaacc gtcatctcag agattatcag
     6901 ttgtaatttg tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg
     6961 cctgtacgcc aataagcgag gggagtagca gtggataaac aaaccctgca ggaaacagtg
     7021 agccagattc tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat
     7081 attcatcttt caacagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa
     7141 aagccgttaa tgcaaccggg ccaattcgct gctgaacagg ttgttacgct ggagggacca
     7201 aaaggcaaat tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa
     7261 atttcccgga cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat
     7321 ctcagcggca ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct
     7381 ggcgtcattg tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggt
     7441 gttacgcacg gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc
     7501 gaaaacgtca ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat
     7561 gaagccaacg ccgcaggggc tgatagccct gacgtttttg ccacactggt gtcagtgaca
     7621 tgaataacat gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg
     7681 cgggaaaaac actcgtgctg actgtttttc aactgcgaga cgccagcgtt caagagagcg
     7741 ttcatcagta tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg
     7801 cggagaacga aacgtcagat cgagcagcaa tccagataca cgaagcgtta gcctggggaa
     7861 tgcacattca attgtcgttg cagcgtcatt tcctgaacgc cattgaattg aagactctcg
     7921 cccgtctacc gctaagctgg tgcgacgaac aagggcaacc gatctaccta catcatgatc
     7981 gacttttaag ttatgtcgat atcgcacagt taagctcggg aatattggtg ttacagcgga
     8041 aatgttgtgt gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca
     8101 ggcaggagta acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat
     8161 caccttctct gatcgggaaa aaactgctgc tggttcgcca ggtcagtgct gacggcgaac
     8221 tgcctctcac gccattaacc ggcgacgaag ttgccgttga ttccgtcggc gcaggcgttg
     8281 gtgagttggt actcctcagc agtggttcca gcgccagaca tgtgttttcc ggccctaacg
     8341 aagccatcga cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggacctgaaa
     8401 gatggcaatt tatacccgaa caggtgattc aggaagtacg tctttattta ccggacaacg
     8461 cgtcagcaaa actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac
     8521 actcagtctg tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat
     8581 tcaacaacag atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc
     8641 gcagcagcgt tacatcggta cggaagaaat tgccgcgctg gaaaacgcga tcgatagcgc
     8701 aatgaacgcc gtccccccgg ttcactgctt cattctgcct ggtcgatgtg aagccgcaag
     8761 ccgcatgcat ttcgcccgca cggtggctcg acgggctgaa agacgtctgg ttgaactgac
     8821 aacagaaacc accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct
     8881 gtatgccctc gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat
     8941 cacaaaacgt tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct
     9001 cacttttcag gatcttcacc agctcattcg tagtgcggca atgcgcgcag atgaactgca
     9061 tattcccgtt gtcatcagca ttgtcgatgc caacggaaca gaaagtgtta cctggcggat
     9121 gcctgatgcc ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc
     9181 gatgaaaacg gcaacccaca agcttgctga taccgtgcaa cccggcgcgc cgctttatgg
     9241 gctcgaaagc catatgcaag gcaaagtcgt cacctttggt ggcggcttcc ccctctggcg
     9301 tgacggaaaa ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga
     9361 tattgcccaa agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa
     9421 ttagaaacac tgatccgcaa tattctgcgt gagcagcttg tccccgctac gcaacgagcg
     9481 caaagaaacg ctattttcca gactgttgat gaagcggtct gcgcagcaca ccaggccttt
     9541 ttacgctttc aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa
     9601 gaattaatgc cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga
     9661 aataaagaag ataagtacct aaaaaacaaa gcggcacttg ataacacgcc tggaattgaa
     9721 gatttaacca ccacagcact gaccggtgat ggaggcatgg ttttgtttga atactctccg
     9781 tttggggtga ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac
     9841 agcatcagca tgctggcagc aggtaatagc gtctacttca gtccacaccc gggagccaaa
     9901 aacgtttcgt taaaactgat taccataatc gaagacatcg cgtttcgctg tagtggcatt
     9961 cacaacctga tcgttaccgt atctgaacca acctttgccg caaccgaaca aatgatggcg
    10021 caccctaaaa tcagcgttct ggcgattacc ggtggcccgg ggatcgtcgc catgggaatg
    10081 aaaagtggca agaaagtgat tggtgccgga gcgggtaacc caccctgcat tgtcgatgaa
    10141 acggcggata ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac
    10201 ctgccgtgta tcgctgaaaa aagcctgatt gtcgtggtat cagtggcaga gtatttaatt
    10261 cagcagatgc aaagctttgg agcacttctt cttaaccatg aacaaacgga gaaattgcgt
    10321 gcaatttgcc tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc
    10381 ttgctggaag cagcgggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt
    10441 gttgacgccg atgatagttg ggttacgtgt gaacaactga tgccaatgct gcccattgtg
    10501 aaggtcaacg attttgacag cgctctcgcc ctggcattga aagtggaaga cggcttgcac
    10561 cacacggcaa ttatgcactc gcagaacgtc tcacggctca accttgccgc gcgcgttatg
    10621 caaacctcaa tctttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa
    10681 ggctttacga cctttaccat tgcaacacca accggagagg gtacaacgtc agcacggact
    10741 tttgcccgct cacgacgctg cgtgttaacc agcggctttt ccattcgcta gagaaacgga
    10801 aatgaaaact ttttcactgc agacgcgttt atacagcgga acgggaagtc tcaacatact
    10861 gaaccgtttc acgaataggc acatctggat tatttgcgac gtctttctgg cacactcacc
    10921 actcattgac acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga
    10981 aataacgccc gacccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt
    11041 acgcccggat gtggttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcgat
    11101 tgtctggttc ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac
    11161 cagcggtacc ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat
    11221 taaatatccc cttttcgata atgcacttta tccggatatc gcgatccttg atccatcttt
    11281 ggtcgtcagc gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc
    11341 gcttgaagct tatgtttctc cccgcgccag cgacttcacg gatgcactgg tcgaaaaagc
    11401 cgtgcaaatt gtttttcaat acctgcccac tgcagtgaag aaaggcgatt gtcttgccac
    11461 ccgtggcaaa atgcataatg catcgacgct tgcaggaata gcctttagcc aggccggact
    11521 cggtttaaac catgccattg ctcatcaact cggcggacag tttcatctgc cgcacggcct
    11581 ggcgaatgcg ctcctgctca cggcggttat ccactttaat gctggcgatc cgcgcgcagc
    11641 caaacgctac gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac
    11701 cgccagcctg aatgcactta ttcaacatat tgagcagctt aaaacaacct gtacgctgcc
    11761 gactcttacc aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat
    11821 ggttcaggcg gcactggcgg atgcaacgtt gcgtaccaac ccgcgtgcgg ctgatgcctc
    11881 cgctattgca gaactgctcg aggagttgtt atgaacgtcg agttaacctc ctgcgacgac
    11941 cagaccatcc gcgagcgagt acaggcagca ggtgtcgtgg gtgctggcgg cgcaggtttt
    12001 ccaacccata ttaagctaca agcgcgtgtt gacacttttc tggtgaatgc ggcggaatgc
    12061 gaaccaatgt tgaaagtgga tcaacagtta atggtgcagc aagccagccg cctgcttcgc
    12121 ggtgttcact atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagag
    12181 aaatatcaac gggcaataaa cgcactcacc cccctattac caccggatat ccgcctgcat
    12241 attttgcccg acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt
    12301 cgccgcgttc cacccgctgc attacccgtt aacgtcggtg tggtggtcaa taatgttcaa
    12361 accgtactta atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc
    12421 actattaatg gtgcagtggc gaaaccaata acggtaaccg tccccatcgg catgtcttta
    12481 cgtgaagtcc tggcgctggc gggcggcgca acagtagatg ctcctggttt tatcaatggc
    12541 gggccgatga tgggaaattt gatcacatcg ctcgacaccc ccgtcagcaa aaccactggc
    12601 ggcttgctgg ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc
    12661 agcgtactta ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc
    12721 ccacgtcatt tagtcggtca cgaactggca ccccatttat tagtacgtgc ggttaactac
    12781 caacagctcg ctacgccaca actgttactg acggcgctca cctgttcgga gtgcaacgtc
    12841 tgcgccagcg tcgcctgtcc ggtaggaatt tcaccaatgc gcataaaccg cctgcttaag
    12901 caggaattac gcgcacaaaa tctccgctac gaaggtgcct taaatcctgc tgatcccatg
    12961 gcaaattatc gactgattcc cgtaaaacgg ctggtaacca gactgggcct aatcccgtgg
    13021 tatcaggatg cgccattgtc tgaacaggtg cctcagcccg aaaaagtgac gttattgtta
    13081 cgccagcaca tcggtgccag cgcaattgcg tgtgtacaaa aaggcgatcg cgtcgttcat
    13141 gggcaatgtg taggtcaaat tccacacggc acattaggtg cccccattca tgccagtatt
    13201 gatggcatgg tcagcgatgt gacagaaaac gcgattacgc ttgtgagagg ttaacgatgt
    13261 cgaaagcggt aggtatttta gaacttagca gtatcgcgaa agggatggaa accggcgacg
    13321 ccatgctgaa aagcgcaaac gttgagttac tggtcagcaa aacgctatgc cccggaaaat
    13381 ttctgctgat gctcggtggc gatgtgggtg cggtgcaaca ggccatcaac agcggtgctt
    13441 cccttgctgg tgaaatgctg gtcgacagtc ttgtcctgcc aaatattcat cccagtctcc
    13501 tttctgcaat cagcggactg aaccatgttg aacagcaccg ggctgtcggc gtagtcgaaa
    13561 cctggagtgt cgccgcctgc attgacgctg ccgattgtgc tgtcaaagct gcgaatgtca
    13621 ttctcgttcg cgtacatatg gcttttggca ttggtggtaa atgctacatg gtggttgctg
    13681 gcgatatttc agatgttaat aacgcggtct ctgtcgccag cgagcgtgcg ggggaaaaag
    13741 gtttgttggt ctatcggtca gtgattgccc gtccacatga agccatgtgg cgtcagatgg
    13801 tggagggata atgacaactc agcacacaac tgaacggatg attcaggaat atgtccctgg
    13861 taagcaggtc acgctggcac atgtcatcgc caatcctggg aaggatctgt ttaagaaatt
    13921 gggtttacca gacaccgttt ctgctattgg cattctgacg attaccccca gcgaagcatc
    13981 tatcatcgca tgtgatatcg ccacaaaatc aggcgctgta gagatcggtt ttcttgatcg
    14041 ttttaccggt gccgtagtgc tgactggtga tgtttctgct attgaatatg cacttagcca
    14101 ggttacccgt acgctgggtg aaatgatgcg ctttaccgcc tgccccatta cgcggacctg
    14161 acgttatgca acgtatgatg cttatcggcc ccagccagtg cggaaaaacg tcactcacgc
    14221 aatgtctgcg tggcgaaaat atccattatc agaaaacgca ggcaatcgtc tggtccccga
    14281 ccaccattga tacaccaggc gaatacctcg aaaaccgcag cctctacagt gcattgttgg
    14341 tcagtgcctg cgaagcagac attatcgcgc tggtacttaa tgcggacgca ccgtggtcac
    14401 ctttttcgcc aggctttacc tcgtcaatga atcgcccggt cattggagta gtaaccaaag
    14461 cagatttagc cagcatggaa caaatatctt tggtgaaatg ttggttacgg gaagctggag
    14521 cgcacaacgt gttagtaacc agtgcggtta ataacaatgg agttacagaa ctttttgcct
    14581 tgctgcatac agaagaaggc tgccgctaat ttcgataggt tctcagatga gaatgatgac
    14641 ggtgatacac agataagaat caatgagcaa tgagggttgc cgggcaaccc tcattgaata
    14701 aaacaggaat tacttcgctt cgccgttttt catttcgccc atagctttca gctttttgga
    14761 gatatcgcgg cgttctttgg agagctcggc atttttgatg atgtaatcgt caacgcgatc
    14821 ttcatagtca cctttcatgc tggcgatgat gccctggatt gcttcaacgc tcatccctgg
    14881 cttgatgtaa tcgctcaggt tgtccagcag caggacgcgc ttctggttgt cacggatctt
    14941 tttctcaacg tcctgaattt cacgttgcag tttgttctta cgacggaaca gacgaacaaa
    15001 ttccagtacg tcctggaatg aaggcttggt agtttccatt tttatacccc tgataatgtg
    15061 agagtcggat tcgtttaagc aaccgctatt cgttagggcc aacattactc atagctgccg
    15121 cagatgacaa tgcttttatc ccttttcact atcataccct ttatccttgc tgaatcgaag
    15181 cagcagcaag atgattctga agttcaggaa ttatttgcgc aaggcccggc aaatcagatt
    15241 cgatagcaac tcaagctgat gtgccgtttc catgttcagc cacacataac catagattgg
    15301 tgtttccaca accttcaggt catggtggtt atcgagcaac tgacgcagct cttccacagc
    15361 atcgttcaat ttttccgtat tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg
    15421 aacaaggctc agcagtattt gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt
    15481 caataacacg aaatggctgg ggcgcgtggc ccagtatgca ttgatttgca actccagcat
    15541 acaaaccaga ttgcgattaa tggtctggat accttcatat atcgattttg gaatacgggt
    15601 ttctttgctg gcgggcgcaa tcagcccacg cattttcacg gcatcggtca ggagtttttg
    15661 tagatggctt tccagacgtg ggcgttcgag taagttcggt gagaatgcag attgatagac
    15721 ccgattatac tcggtcagac ttttcgccag ttgaatgcgc cagtggatga acgcccgttg
    15781 tggccagata ccggtaaaca acattgccag taaagagccg aggatcacat cgccgcttcg
    15841 ccataacgcc gtatcaattt cacctgtcgg ggaacccaca acaattgcca gcgtcacccc
    15901 aatcaataaa ccttgatatg gtttcttgcc cagcgccagc caaccgcaaa ggaacatggc
    15961 cgccgcgcac cagactaaca tcagcggtaa cgagattaac tccagttgca gagcgataag
    16021 acctaaaatc gaccccaaca ccgtaccgcc aatacgttcg aaggcacgag ggacaacgtt
    16081 cccccagaac gagattggcc ccataatcac caccatggtg accagcggcc aggtgctttc
    16141 cgggatagta aacaggcgga taatgagaaa agtgagcagg aatgccagcg cgacccgagt
    16201 accatgcaca atgcggtaat ggcggtatac ccggatttca aacgggctta atgacttatc
    16261 ggcacgcacg cagaaactcc gtaatgaaga ttaaaagcgc tcataactca tctgtcggcc
    16321 agaaggcgcg cctctgaatt atccgacgga caaaccggga acagtgtaat ttcccggcag
    16381 gagcgaaaaa attgaaaggc gcatcttact cttttcgctt tcaaaaaaag agtggtcttc
    16441 gcgtcaacac accgccctga gatgaattag tgatgttttg gctgcaccgc aacatacatt
    16501 tcgatatccc agtacccatc ttccgcgcca ttgttcaagt aaacctcgaa gcagggcttt
    16561 ggtaacattt cataagcact gtcctgcaag aggctattaa agaactgata ccagggttta
    16621 gcaaaatcat caccgactac acgagccacc gccaccgcat actgaccacc tgaaatttct
    16681 gtcagaatga cgccctcact gttttcgggg agcgtaaagt tattcggtac cgtcacgacg
    16741 gtgtcgcagc gtaatttttc ggcgggtgtt tcatctggat tgtcgtaata gacagcaacc
    16801 cactccttcg gcacaatatt tttgctatct atccacatca tcaactgctc aaaacctttc
    16861 tttaccgtct gctcccacgg gccaacaaga tggaaacctg caacggtacg tttctcttcc
    16921 tgcttaatct cgtagttcat gacgcctcca ttaacactgt ttttatatac agtatagttg
    16981 caatattaaa accacaagga atgagtgttg attatgcgag cagactcgca ctcctgccag
    17041 tctgctgcaa aagaaaggtc aggccttatg gtggaaataa tcactcaggc gggaaaacat
    17101 gccgccttcc ccgacagatt ccagggtaac cagcggccag tgcgccacct gtttatcacg
    17161 gtcgtaaagt tcaatttccc ctacccgctg atgggcgcta attggcgcgg tgagctcttt
    17221 accatcaagg gtatatttgg ctttgatatg tggaatttcg gctttcggta gcaccatcca
    17281 gaactcttgt tccgttccca ggtcgatatt ttctttatcg ccataccaga tgcgttccgt
    17341 tccgaccttt ttcccacggt gcaaaatttg caccgtagta aagttttgtt gcccccaacg
    17401 cagtaatttt cttgcctctt cctcacgacc ttttgcactg tcagccccca ttaccactgc
    17461 aatgagacga cgctgcccat ctacagccga agcaatgaga ttaaacccgg caccagaagt
    17521 atgacccgtt ttcaggccgt caacattcat ggttttatcc cacaacaacc cgttacggtt
    17581 ttgctgggtg ataccgttcc aggtgaggct tttctcactg tacatatgat aaaactcggg
    17641 ctcgccgtgg atgatagcgc gagaaagcac agctaaatca taagccgagc tatgctggcc
    17701 aggtgcatcc agaccatgca ctgtttcaaa atgcgtatcc ttgagatgca gcttctcggc
    17761 atagttgttc atcattgcaa caaactgccg ttgcccaccg gcaatatagt cagccagtgc
    17821 aacacaagca tcatttccgg aatccacaat taaaccacgg cttaaatcac gtaccgatac
    17881 gcgatcgccc tctttcaaaa acatcagtga agaaccgaca aacactggat tatctttcgc
    17941 ccacgcatcg cgccctacgg tgacaatatc gtctggcgta attcgatgac tatcgatagc
    18001 gcgatccacg acataacccg tcatcagctt tgtcaggctg gcgggattgc gctgttgatg
    18061 ctcattaccc gcggtgagga tctgtccggt ggtgtaatcc atcaataccc aggacccggc
    18121 atgaatctct ggaggctgag gtgaaaaagg aatgttttcc gccgcaaaac cagacgataa
    18181 gttaaaaacg aacaaagaag cagcaataat aagacggcgt ttcaacagca aaccctcagg
    18241 agtttcaaat agctgttctt tttacggaaa tacttatgaa ctggctggaa taaagtgcaa
    18301 gaaaatgtga ctaacctctc atttttatct gacatgatct gttgccactc gctgccaaat
    18361 tgtggcgcta aagctgatta gcacggtgat atttgatact ctggcagaca gcagagataa
    18421 cggatttaac ctaatgatga atgacggtaa gcaacaatct acctttttgt ttcacgatta
    18481 cgaaaccttt ggcacgcacc ccgcgttaga tcgccctgca cagttcgcag ccattcgcac
    18541 cgatagcaaa ttcaatgtca tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga
    18601 ctatttaccc cagcctggag cagtattaat taccggtatt accccgcagg aggcacgggc
    18661 gaaaggagaa aacgaagccg cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa
    18721 gacctgtatt ctgggctaca acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt
    18781 ttatcgtaat ttctacgatc cttacgcctg gagctggcag catgataact cgcgctggga
    18841 tttactggat gttatgcgtg cctgttatgc cctgcgcccg gaaggaataa actggcctga
    18901 aaatgatgac ggtctaccga gctttcgcct tgagcattta accaaagcga atggtattga
    18961 acatagcaac gcccacgatg cgatggctga tgtgtacgcc actattgcga tggcgaaact
    19021 ggtaaaaacg cgtcagccac gcctgtttga ttatctcttt acccatcgta ataaacacaa
    19081 actgatggcg ttgattgatg ttccgcagat gaaacccttg gttcacgttt ccggaatgtt
    19141 tggggcatgg cgcggcaata ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg
    19201 caatgccgta attatggtgg atttggcagg agacatttcg ccattactgg agctggatag
    19261 cgacacattg cgcgagcgtt tatataccgc aaaagccgat cttggcgata acgccgccgt
    19321 tccggttaag ctggtgcata tcaataaatg tccggtactg gcccaggcga atacgctacg
    19381 cccggaagat gccgaccgac tgggaattaa tcgtcagcat tgcctcgata acctaaaaat
    19441 tctgcgtgaa aatccgcaag tgcgtgaaaa agtggtggcg atattcgcgg aagccgaacc
    19501 gtttacgcct tcagataacg tggatgcaca gctttataat ggctttttca gtgacgccga
    19561 tcgtgcagca atgaaaattg tgctggaaac cgagccgcgt aatttaccgg cactggatat
    19621 cacttttgtt gataaacgaa ttgaaaaact gttgttcaat tatcgagcgc gcaacttccc
    19681 ggggacgctg gattatgccg agcagcaacg ctggctggag caccgccgcc aggtcttcac
    19741 gccagagttt ttgcagggct acgctgatga attgcagatg ctggcacaac agtatgctga
    19801 tgacaaagag aaagtggcgc tgttaaaagc gctttggcag tacgcggaag agatcgtctg
    19861 aaacaaaaaa gccggagcga caaactccgg ctttttgaga tttaccggat gattatgtca
    19921 tccggtttgt aagattacgc tacgtcttcg tactgcggaa ccgggttacg gaaactcttg
    19981 gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag aaccattgag
    20041 ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat cggcaggaac
    20101 agatactgga agtgatcttt cagcgtcttg ttacgcttct cacggatcca gaactgcgag
    20161 atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat cagcgccgta
    20221 gccattacca ggtcgaagtt gattgccagc agggcaatcg cgccaaccag gatgatgttc
    20281 atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg gaacacgccg
    20341 tcacgaccca ttacgtacat cagacgcgct acgcctgcgt gcgccgccat accggacgcc
    20401 agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc cgcaacgtac
    20461 agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc cgggaagtac
    20521 agctgcagga agtaagttgc aaagatgaag atcatgccgc cgatcagcgc ggtcaggaaa
    20581 atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt gctgatgccg
    20641 tcaaaaccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg gataacatgc
    20701 gcatcgccag accagaacgg acgagtgctc gccagcgtac cagcgccttc accttcaaat
    20761 acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa cacgacgatt
    20821 acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt cataaaggcc
    20881 accagcgcca ccacaaacat ccacgatgga atggaaggca ccagagcttc aaaatagatt
    20941 ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga agaccaaccc
    21001 accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc agagccagca
    21061 gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat cgcaatcaat
    21121 gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat accaaaagta
    21181 tcaaacagcg tcatcggctg catataggct agacccatca ttacaaccgg aactaacgta
    21241 agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga catggtcatt
    21301 ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt tggattcctc
    21361 agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc ggcctcttgg
    21421 tatgaatgat tggtttgaag caaaaaataa ccgacgctaa ttaaacgtcg gtttttagtc
    21481 attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag aatgagaggc
    21541 ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt gcataaacac
    21601 cggcgatcgc actatttgct aaaatttcgt cccgccacca accactggca agccctgcgg
    21661 tctgttcatt ccagccaatc cagacgggtt caaagagagt ttgggcgacc actttcttct
    21721 cgattaacgc gccactatcg agaaaacgtt gcgccagata ccgtggtaaa tagccgcagc
    21781 caaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta atggcttctt
    21841 gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtgtcc cccaccacaa
    21901 tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttcctgg gctaacggat
    21961 gatgaggcgc gaccgcaaag acttgttcaa gatcgcccaa ccgcgagaaa ccaaactcac
    22021 tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc tgagtcagtg
    22081 catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg ctatgatgtt
    22141 gataaaaagc ctcaatgaga ggcgcaagca gagaaaaagg aaacgtgtcg tccacgccaa
    22201 tcaccagctc attttcccag ccttcatgca gtttgatagc ctgcttttcc agttctcgca
    22261 cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg gtgaatttag
    22321 cgcggtgacc gctacgatca agcaattgaa tattaaggtc gctttccagc ttgtgaacgg
    22381 tataactcag agcggacggc gtcttgtaga gtttagccga cgccgccgca aaactgcctt
    22441 ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata cacgccccct
    22501 tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt accagcggat
    22561 cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca ataatcttac
    22621 ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccagt aaacgggcca
    22681 tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta tagatgtgtc
    22741 cgcctttagg tgcctgtaac aacagagtga tagcaccaat aacatcttct aaatggacta
    22801 aattaacacc atgttcacca tcaggggcgg tttttccggc aaagaagcgt ccgggatgac
    22861 gtcccggtcc gaccagcccc gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt
    22921 gcagccagtc ttcgagttct tctaatactc gtccactgtt ggtcaccgga ttacgcgggg
    22981 tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgttgagctg gtaaaaataa
    23041 ttcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct tgtaaataga
    23101 actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgcg tcggcatcca
    23161 tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga agcagatagc
    23221 tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta ctcccggtga
    23281 cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac cctaacccga
    23341 caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata aggctacgct
    23401 agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg ataacgacat
    23461 gctgcaatga gtagtgaatc gtcggtaacc gcgtatacca gacggtgctc ctctgtaatg
    23521 cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggtttccc cttaccttca
    23581 aatggtgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat tcgcttatct
    23641 gtttcctgcc agtacagata atcatcccat gattcctcag accagattag tttcactcaa
    23701 tgatgtcctt ttccgttcct ttgccagatt tcaggctatc gattgagtcc atcaatctcc
    23761 gggcgttagc gggggagcgc agtagataag ccgtctcctc cagggagttg tattcttcga
    23821 gtgacatcag aacacaagcc tctccattct gacgagtaat aaggatcggg gcatgatctt
    23881 caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag ctaattgtac
    23941 gcatgtcaat ctcctctttt gtacagttca ttgtacaatg atgggcgtta attaactatt
    24001 tattaattag tttgtagatc aaggtattgt cagtgagacg aaaatccagg cttcgctatt
    24061 tttggtgccg tcagcttaga ggacagtcct cttagccccc tcctttcccc gctcattcat
    24121 taaacaaatc cattgccata aaatatataa aaaagccctt gctttctaac gtgaaagtgg
    24181 tttaggttaa aagacatcag ttgaataaac attcacagag acttttatga cacgcgttca
    24241 atttaaacac caccatcatc accatcatcc tgactagtct ttcaggcgat gtgtgctgga
    24301 agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga agatcacctt
    24361 ccgggggctt ttttattgcg cggttgataa cggttcagac aggtttaaag aggaataaga
    24421 aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt ttaagtgatg
    24481 actcacgcga attgctggcg cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga
    24541 tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgac gacattcccg
    24601 gtctggtaat ggacggcgtg gtagaccttg ggattatcgg cgaaaacgtg ctggaagaag
    24661 agctgcttaa ccgccgagcc cagggtgaag atccgcgcta ctttaccctg cgtcgtctgg
    24721 atttcggcgg ctgccgcctt tcgctggcaa cgccggttga tgaagcctgg gatggcccgc
    24781 tctccttaaa cggtaaacgt atcgccacct cttatcctca cctgctcaag cgttatctcg
    24841 accagaaagg catctctttt aaatcctgct tactgaacgg ttctgttgaa gtcgccccgc
    24901 gtgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg ctggaagcaa
    24961 acggcctgcg cgaagtcgaa gttatctatc gctcgaaagc ttgcctgata caacgcgatg
    25021 gcgaaatgga agaatccaaa cagcagctga tcgacaaact gctgacccgt attcagggcg
    25081 tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgaccgaa cgcctggatg
    25141 aagtcatcgc cctgctgcca ggtgccgaac gcccaactat tctgccgctg gcaggtgatc
    25201 aacagcgcgt agcgatgcac atggtgagta gcgaaaccct gttctgggaa acgatggaaa
    25261 aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc gattgaaaaa atgatggagt
    25321 gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcgg agcaacaacg
    25381 ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca ccgttaacga
    25441 tatcctcgat aacgtgaaag cacgcggcga tgaggccctg cgtgaataca gcgcgaaatt
    25501 tgataaaacc acggttaccg cgctgaaggt gtctgctgag gagatcgccg ccgccagcga
    25561 acgcctgagc gacgagctaa aacaggcgat ggcggtggca gtaaagaata ttgaaacctt
    25621 ccacactgcg caaaaactgc cgccggtaga tgtcgaaacg cagccaggcg tgcgttgcca
    25681 gcaagtcacg cgcccggtcg cttcagttgg gttgtatatt cctggcggct ccgccccgct
    25741 cttctcaacg gtattaatgc tggcaactcc ggcgcgtatt gcgggctgta aaaaagtggt
    25801 gctgtgctca ccgccgccga ttgccgacga aattctttac gcggcgcagc tgtgcggtgt
    25861 gcaggatgtg tttaacgtcg gcggcgcaca ggccattgcc gcactggcgt ttggtacgga
    25921 atctgtgccg aaagttgaca aaatcttcgg gccaggtaac gcctttgtca ccgaggcaaa
    25981 acgtcaggtg agccagcgtc tggacggtgc ggcgatcgat atgcccgcag gcccgtcgga
    26041 agtgctggtg attgctgaca gcggcgctac gccggatttc gtggcttctg atttgctttc
    26101 tcaggctgaa cacggcccgg actcgcaggt gattttactg acgcccgatg ctgatatggc
    26161 gcgtcgcgtt gccgaggctg tcgaacgcca actggcagaa ctgccgcgtg ccgaaaccgc
    26221 ccgccaggca ctgaacgcca gccgcctgat cgtgactaaa gatttagcgc agtgcgtaga
    26281 gatctccaac cagtacggcc cggagcacct gatcatccag acccgcaacg cccgcgatct
    26341 ggtcgatggc atcaccagcg ccggttcggt atttcttggt gactggtcac ccgaatcggc
    26401 gggtgattac gcctccggca ccaaccacgt actgccgact tacggttaca ccgccacctg
    26461 ttccagcctc gggctggcag atttccagaa gcgtatgacc gtgcaggaac tgtcgaaaga
    26521 aggtttctcc gcgctggctt cgaccataga aacactggcc gccgccgagc gcctgaccgc
    26581 ccataaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat gagcaccgtg
    26641 actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca gtcggcgcgt
    26701 cgtctgggcg gtaacggcga cgtctggctg aacgccaacg aataccccac agccgtggag
    26761 tttcagctca ctcaacaaac gctcaaccgc tatccggaat gtcagccaaa agcggtgatt
    26821 gaaaattacg cgcaatatgc aggcgtaaaa ccggaacagg tgctggtcag ccgtggcgcg
    26881 gacgaaggta ttgaactgct gattcgcgct ttttgcgaac cgggtaaaga cgccatcctc
    26941 tactgcccgc caacgtacgg catgtacagc gtcagcgctg aaaccattgg cgtcgagtgc
    27001 cgcacagtgc cgacgctgga caactggcaa ctggacttac agggcatttc cgacaagctg
    27061 gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc cgaccgggca actgatcaac
    27121 ccgcaggatt ttcgcactct gctggagtta acccgcggta aagcgattgt ggttgccgat
    27181 gaagcctata tcgagttttg cccgcaggca tcgctggctg gctggctggc ggaatatccg
    27241 cacctggcta ttttgcgcac actgtcgaaa gcttttgctc tggcgggcct tcgttgcgga
    27301 tttacgctgg caaacgaaga agtcatcaac cttctgatga aagtgatcgc cccctacccg
    27361 ctctcgacgc cggtcgccga cattgcggcc caggcgttaa gcccgcaggg gatcgtcgcc
    27421 atgcgcgaac gggtggcgca aattattgca gaacgcgaat atctgattgc cgcattgaaa
    27481 gaaatctcct gcgtggagca ggtttttgac tctgaaacca actacattct ggcgcgcttt
    27541 aaagcctcca gcgcagtgtt taaatctttg tgggatcagg gcattatctt acgtgatcag
    27601 aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg tgaagaaagc
    27661 cagcgcgtca ttgacgcctt acgtgcggag caagtttgat gagtcagaag tatcttttta
    27721 tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg gaccgttttg
    27781 acaaactcgc ctttgaaccg ggcgtgatcc cggagctgct gaagctgcaa aaagcgggat
    27841 ataagctggt gatgatcacc aatcaggatg gtctgggaac acaaagtttc ccgcaggcgg
    27901 attttgatgg cccgcacaac ctgatgatgc agatcttcac ctcgcaaggc gtgcagtttg
    27961 atgaagtgct gatttgcccg cacctgcccg ccgatgagtg cgactgccgt aaaccgaaag
    28021 taaaactggt ggagcgttat ctcgctgagc aagcgatgga tctcgccaac agttatgtga
    28081 ttggcgatcg cgcgaccgat attcaactgg cggaaaacat gggtattaat ggtttacgct
    28141 acgaccgcga aaccctgaac tggccgatga ttggcgagca actcactaaa cgagaccgtt
    28201 acgcccatgt agtgcgcaac accaaagaga cgcagattga cgttcaggta tggctggatc
    28261 gcgaaggtgg cagcaagatt aacaccggcg ttggcttctt tgatcatatg ctggatcaga
    28321 tcgccactca cggcggtttc cgcatggaaa tcaacgtcaa aggcgacctc tatatcgacg
    28381 atcaccacac cgtcgaagat accggcctgg cgctgggtga agcgttaaaa attgccctcg
    28441 gcgataaacg cggtatttgc cgctttggtt ttgtgctgcc gatggacgaa tgccttgccc
    28501 gctgtgcact ggatatctct ggtcgcccac acctggaata taaagctgaa tttacctacc
    28561 agcgcgtcgg cgatctcagc accgagatga tcgagcactt cttccgttcg ctctcttaca
    28621 ccatgggcgt gacgttgcac ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaga
    28681 gtctctttaa agcctttggt cgcaccctgc gccaggccat ccgcgtggaa ggcgacaccc
    28741 tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc cttgataccg gctgcgccaa
    28801 tttgaactcg gtgaagtcag ccattgcgcg tcacggttat gaacccaaag tcagccgtga
    28861 cccggacgtc gtgttgctgg ccgataaact atttttaccc ggcgtaggca ccgcacaagc
    28921 agcgatggat caggtgcgtg agcgcgagct gtttgatctc atcaaagcct gtacccaacc
    28981 ggtgctgggc atctgcttag ggatgcaact gctggcgcgg cgcagcgaag agagcaacgg
    29041 cgtcgatttg ctgggcatta tcgacgaaga cgtgccgaaa atgaccgact ttggtctgcc
    29101 cctgccgcat atgggctgga accgcgttta cccgcaggca ggcaaccgcc tgtttcaggg
    29161 gattgaagac ggcgcgtact tttactttgt tcacagctac gcaatgccag tcaatccgtg
    29221 gaccatcgcc cagtgtaatt acggcgaacc gttcaccgcg gcggtacaaa aagataactt
    29281 ctacggcgtg cagttccacc cggagcgttc tggtgccgct ggcgctaagc tgctgaaaaa
    29341 cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac cgtggtgcgt
    29401 ctccatcagg gagattatgg caaacaacgc gattacggta acgacccgct gccacgctta
    29461 caggattacg ccgcgcaggg tgccgaagtg ttgcatctgg tggatctgac cggggcaaaa
    29521 gatccggcta gacgtcaaat cccgctgatt aaaaccctgg tggcggacgt taacgttccg
    29581 gtgcaggttg gcggcggcgt gcgtagcgaa gaagacgtgg cggcgttact ggaagcgggc
    29641 gttgcgcgcg tggtggttgg ctccaccgcg gtgaaatcac cagaaatggt gaaaggctgg
    29701 tttgaacgtt tcggtgccga tgccttagtg ctggcgctgg atgtccgtat tgacgagcaa
    29761 ggcaacaagc aggtggcggt gagcggctgg caagagaact cgggcgtttc actggaacaa
    29821 ctggtggaaa cctatcttcc cgtcggcctg aaacatgtgc tatgtaccga tatctcgcgc
    29881 gacggcacgc tggcaggttc taacgtctct ttatatgaag aagtgtgcgc cagatacccg
    29941 caggtggcat ttcagtcctc cggcggcatt ggcgacatta acgatgttgc tgccctgcga
    30001 ggtactggcg tgcgcggcgt aatagttggt cgggcattac tggaaggtaa attcaccgtg
    30061 aaggaggcta tcgcatgctg gcaaaacgca taattccatg tctcgacgtt cgtgatggtc
    30121 aggtggtgaa aggcgtacag tttcgcaacc atgaaatcat tggcgatatc gtgccgctgg
    30181 caaaacgtta cgccgaagaa ggtgcagacg aactggtgtt ctacgatatc accgcttcca
    30241 gcgatggccg tgtggtagat aaaagctggg tatctcgcgt ggcggaagta atcgacattc
    30301 cgttttgtgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt ctttcctttg
    30361 gcgcggataa aatctccatt aactcccctg cactggcaga cccgacgtta attactcgcc
    30421 tggcggatcg ctttggcgtg cagtgtattg tggtcggcat tgatacctgg tacgacgccg
    30481 aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc cgcgtcactc
    30541 agtgggaaac actcgactgg gtgcaggaag tgcaaaaacg cggtgccgga gaaatcgtcc
    30601 tcaacatgat gaatcaggac ggcgtgcgta acggttacga cctcgaacag ctgaaaaaag
    30661 tgcgtgaagt ttgccacgtc ccgctgattg cctccggtgg cgcgggcacc atggaacact
    30721 tcctggaagc cttccgcgat gccgacgttg acggtgcgct ggctgcttcc gtattccaca
    30781 aacaaataat caatattggt gaattaaaag cgtacctggc aacacagggc gtggagatca
    30841 ggatatgtta acagaacaac aacgtcgcga actggactgg gaaaaaaccg acggactgat
    30901 gccggtgatt gtgcaacatg cggtatccgg cgaagtgcta atgctgggct atatgaaccc
    30961 ggaagctctg gacaaaaccc tcgaaagcgg caaagtcacc ttcttctcgc gcactaaaca
    31021 gcgactgtgg accaaaggcg aaacgtcggg caattttctc aatgtggtga gtattgcccc
    31081 ggactgcgac aacgacacgt tactggtgct ggcgaatccc atcgggccaa cctgccacaa
    31141 aggcaccagc agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga
    31201 acaactgctc gccgagcgca aatctgccga tccggaaacc tcctacaccg ccaaactgta
    31261 tgccagcggc accaaacgca ttgcgcagaa agtgggcgaa gaaggcgtgg aaaccgcact
    31321 ggcagcaacg gtacatgacc gctttgaact gaccaacgag gcatctgatt tgatgtatca
    31381 cctgctggtg ctgttgcagg atcaggatct ggatttaacg acggtaattg agaacctgcg
    31441 taaacggcat cagtgagttg cgggtgatca gatgcgatgt tgttgtcgca tccggcaaaa
    31501 aaaacgggca aggtgtcacc accctgcccg ttttctttaa aaccaaaaga ttacttcgcg
    31561 ttgtaattac gcagagcgtt acgccccagc acaatccccg cgccaaccat gccgcccagc
    31621 agcactgcca gaatcaaggt aattgccttt ttcgggctat cgcgacgaat aggtaacgtc
    31681 ggtttcatca catagcggta agcatgaata tcaagatcat caacctttaa gttttcaata
    31741 tccaacaggt tttgacgagt ctgatagtag tttggcgaga acaccaacgg acgagtcgcc
    31801 tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa ggaacaacgt atcttgtgtc
    31861 acatcttcag tctgctgaac ctgtggcttt gtcacctgcg cctgattcgc atactgcaac
    31921 gcttcctgaa tctgacggat acgcagatct ttctgctctt gcgcgactac ttcctgagtt
    31981 cttaaagagt cctgcaagtt tttcatacgc agcgcgatgt tgtctttcag gtctttttcc
    32041 agttcctcat taacctggtc gtcaacttgc tggatatact tcgccagctg cttctgagca
    32101 ccttcaggtg tttcaccaac ataagacacc gctaaaggta atgactgatt tttaacggta
    32161 ggctcaatgg tcagcttttc tgcttcttcc tgattatcca gcgtttccgc taatgctgaa
    32221 aatgcagtgc tgtaacgtcc gataagtgaa gcctgaatgt cggagacttt cggtacagcc
    32281 gatccgtaga tcacattcat cgcgttggta taaccagcga tttgccccac atcaggttga
    32341 gtaacgatag ctgtggaagt ccacttttct ttggcaacgg ccagataccc tatagccagg
    32401 gcaatagcaa caatgacaga aatgatgata gtcatcttgc cgcgccacaa ctgcaccagt
    32461 aaatcaatca aatcaatctg ttccgggtca tggttttgcc cagaaacatt attattttct
    32521 actctcatca ttaccctaac ggacgaaata attttaagtc gccatagttt agcgataatc
    32581 gccatgaatg ctataggata aatgataaaa agaatgaatt attacaaagg gaacatgaga
    32641 gaatcagggc gaaaatcgcc ctgataacag gatgttagtc actgccaaag agatcgcggg
    32701 tgtagacttt gtctgccaca tccttaagct cttctgccat acggttggaa ataataacgt
    32761 cggcttgttg tttgaaggtg gcgagatcac gttccagtcg agagttgaag aatgaatctt
    32821 ctttcatcac cggctcgtag atgatcactt caacaccttt tgccttgata cgtttcataa
    32881 tcccctgaat ggaagacgcg cggaagttat ctgacccgct cttcataatc agacgataaa
    32941 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatct ttacgcgtgc
    33001 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactggtag ttcgccagta
    33061 actgcttggt atctttcggt agacaataac caccatagcc aaacgacgga ttgttgtaat
    33121 ggttgccaat acgcggatcg agacaaacac cttcgataat ctgacgagta ttcagaccta
    33181 aactttctgc atagctatcc agctcgttaa agtatgctac gcgcatcgcc agataggtat
    33241 tagcaaacag tttaatcgct tctgcttcag tggagtcggt aaacagggtc gggatatttt
    33301 gcttaatcgc gccttcctgt aataacgccg caaaacgttc tgcgcgttct gaacgctcac
    33361 cgatgacaat acgtgacgga tgaaggttat cgtaaagagc tttaccttca cggagaaatt
    33421 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    33481 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    33541 ttactgattc tacactcgat gtattgaaat aattagtttt aggatcatag tcggttggag
    33601 tggcgatgat gacgtaatca gcatcccgat aggcttcatt tttatctaat gtggcattaa
    33661 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    33721 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    33781 gtgcgattag aagcccgttt gacaagccga catagccagt accggaaatg gtgattttca
    33841 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    33901 gttaaaagtt ttaacgctac gttgtaagat ggtcaacgca acgaaaaaca attcagaatt
    33961 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    34021 agataattgg taaaaaaacc cggcataata tacgccgggc ctcaatttta ttgttgatta
    34081 aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg cgcttataag
    34141 tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga acagcggcac
    34201 ggtagctgtc ataataggca accgcagcgg cgaaggtcgg aaccgggata ccgttctgta
    34261 ctgcataagc aacgacatcg cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt
    34321 aaggagccag cagcaggtta gcgatctgcg gattttcggc ataagcatcg gtgattttct
    34381 gcaggaactg cgcacggata atgcagccag cacggaaaat cttcgcgatt tcgccatagt
    34441 tcagatccca gttgtactct tcagacgctg cgcgtagctg agagaagccc tgagcgtaag
    34501 aaacgatttt gcccagatac agcgcacgac gaactttttc gatgaactca gccttgtcgc
    34561 ctgctgactg agcttgcgga ccagagagaa ctttagatgc ggcaacgcgc tgatctttca
    34621 gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt tcgccgagat
    34681 ccagcgcact ctggctggtc catttaccgg tacctttgtt agccgcttca tccagaatca
    34741 catcaaccag gtagttaccg tcttcatctt ttttggtgaa gatatctttg gtgatgtcga
    34801 tcaggtagct gcttagttca ccgttattcc actcggtaaa cgtctgcgcc agttcttcgt
    34861 tggtgagatt caggccacct ttaagcagag aataggcttc agcaatcagc tgcatatcgc
    34921 cgtattcaat accgttgtga accatcttca catagtgacc tgcaccatcg gcaccaatat
    34981 aggtaacgca tggctcaccg tcttcagcca ctgcggcgat tttggtcagg atcggtgcaa
    35041 ccagttcata ggcttctttc tgcccaccag gcataatgga aggacctttc agcgcacctt
    35101 cttcaccacc ggaaacaccg gtaccgataa aattaaagcc ttcggcagaa agctcacggt
    35161 tacgacgaat ggtgtcctgg aagaaggtat taccaccatc aatgatgatg tcacctttat
    35221 cgaggtatgg cttgagggaa tcaatagcag catccgtgcc tgcacctgct ttcaccatta
    35281 acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta tagtaaggaa
    35341 ccagtttctt gcctggattt tcgacaatca cttcttccgt cttttcacgg gaacggttga
    35401 aaatagagac ggtataacca cgactttcga tgttgagcgc gaggttgcgc cccatcactg
    35461 ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgtcag ggttttaccg
    35521 catgtgcggt atgagaataa cttaaaaagt ttacttgaaa taaagtatta tatatatgat
    35581 attgacggta aaaacaaagt tgtcgacaga ctgaatcaaa tggacataca attgagtcct
    35641 attcattaaa ttagtaagcc gtataaatta aatacgcacc ctataaaaca acaacttctc
    35701 aataattaat tgagatccat aataaaacaa aaaaatcaat ctaataatta tcaaattaac
    35761 aaaaaaactt ttttcaataa aaaatgaaga aagatgccta atgctttaaa tttcacacaa
    35821 acaataaaac caacaaaaat aagcaacgta ttgttttatt attactatcc ccaacaataa
    35881 tcacataaaa tgaaagtaat gtatatacga aagatataaa c
//
LOCUS       DAZGTJ010000044        35766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000044 DAZGTJ010000000
VERSION     DAZGTJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.044"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(145..3294)
                     /gene="acrB"
                     /locus_tag="ACHK8E_002708"
     CDS             complement(145..3294)
                     /gene="acrB"
                     /locus_tag="ACHK8E_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP0344955.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3317..4510)
                     /gene="acrA"
                     /locus_tag="ACHK8E_002709"
     CDS             complement(3317..4510)
                     /gene="acrA"
                     /locus_tag="ACHK8E_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP0344956.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATTLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4652..5299
                     /gene="acrR"
                     /locus_tag="ACHK8E_002710"
     CDS             4652..5299
                     /gene="acrR"
                     /locus_tag="ACHK8E_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP0344957.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5427..8789
                     /gene="mscK"
                     /locus_tag="ACHK8E_002711"
     CDS             5427..8789
                     /gene="mscK"
                     /locus_tag="ACHK8E_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP0344958.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8850..8948
                     /locus_tag="ACHK8E_002712"
                     /pseudo
     CDS             8850..8948
                     /locus_tag="ACHK8E_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9001..9162)
                     /gene="rsmS"
                     /locus_tag="ACHK8E_002713"
     CDS             complement(9001..9162)
                     /gene="rsmS"
                     /locus_tag="ACHK8E_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP0344959.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9176..9703)
                     /gene="priC"
                     /locus_tag="ACHK8E_002714"
     CDS             complement(9176..9703)
                     /gene="priC"
                     /locus_tag="ACHK8E_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP0344960.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9773..10150
                     /gene="ybaN"
                     /locus_tag="ACHK8E_002715"
     CDS             9773..10150
                     /gene="ybaN"
                     /locus_tag="ACHK8E_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP0344961.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10303..10854
                     /gene="apt"
                     /locus_tag="ACHK8E_002716"
     CDS             10303..10854
                     /gene="apt"
                     /locus_tag="ACHK8E_002716"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP0344962.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10983..12914
                     /gene="dnaX"
                     /locus_tag="ACHK8E_002717"
     CDS             10983..12914
                     /gene="dnaX"
                     /locus_tag="ACHK8E_002717"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP0344963.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12247..12311
                     /gene="dnaX"
                     /locus_tag="ACHK8E_002717"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12967..13296
                     /gene="ybaB"
                     /locus_tag="ACHK8E_002718"
     CDS             12967..13296
                     /gene="ybaB"
                     /locus_tag="ACHK8E_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP0344964.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13296..13901
                     /gene="recR"
                     /locus_tag="ACHK8E_002719"
     CDS             13296..13901
                     /gene="recR"
                     /locus_tag="ACHK8E_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP0344965.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14011..15885
                     /gene="htpG"
                     /locus_tag="ACHK8E_002720"
     CDS             14011..15885
                     /gene="htpG"
                     /locus_tag="ACHK8E_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP0344966.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16066..16710
                     /gene="adk"
                     /locus_tag="ACHK8E_002721"
     CDS             16066..16710
                     /gene="adk"
                     /locus_tag="ACHK8E_002721"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP0344967.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16842..17804
                     /gene="hemH"
                     /locus_tag="ACHK8E_002722"
     CDS             16842..17804
                     /gene="hemH"
                     /locus_tag="ACHK8E_002722"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP0344968.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17801..18760)
                     /gene="aes"
                     /locus_tag="ACHK8E_002723"
     CDS             complement(17801..18760)
                     /gene="aes"
                     /locus_tag="ACHK8E_002723"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP0344969.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18912..20216
                     /gene="gsk"
                     /locus_tag="ACHK8E_002724"
     CDS             18912..20216
                     /gene="gsk"
                     /locus_tag="ACHK8E_002724"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP0344970.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20300..21976)
                     /gene="ybaL"
                     /locus_tag="ACHK8E_002725"
     CDS             complement(20300..21976)
                     /gene="ybaL"
                     /locus_tag="ACHK8E_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP0344971.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22214..23434)
                     /gene="fsr"
                     /locus_tag="ACHK8E_002726"
     CDS             complement(22214..23434)
                     /gene="fsr"
                     /locus_tag="ACHK8E_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP0344972.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23652..25304
                     /gene="ushA"
                     /locus_tag="ACHK8E_002727"
     CDS             23652..25304
                     /gene="ushA"
                     /locus_tag="ACHK8E_002727"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0344973.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25341..25820)
                     /gene="ybaK"
                     /locus_tag="ACHK8E_002728"
     CDS             complement(25341..25820)
                     /gene="ybaK"
                     /locus_tag="ACHK8E_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP0344974.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26024..26818)
                     /gene="ybaP"
                     /locus_tag="ACHK8E_002729"
     CDS             complement(26024..26818)
                     /gene="ybaP"
                     /locus_tag="ACHK8E_002729"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP0344975.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26951..27253
                     /locus_tag="ACHK8E_002730"
     CDS             26951..27253
                     /locus_tag="ACHK8E_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0344976.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27289..27630
                     /gene="ybaQ"
                     /locus_tag="ACHK8E_002731"
     CDS             27289..27630
                     /gene="ybaQ"
                     /locus_tag="ACHK8E_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0344977.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27688..30192)
                     /gene="copA"
                     /locus_tag="ACHK8E_002732"
     CDS             complement(27688..30192)
                     /gene="copA"
                     /locus_tag="ACHK8E_002732"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP0344978.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30455..31387
                     /gene="glsA"
                     /locus_tag="ACHK8E_002733"
     CDS             30455..31387
                     /gene="glsA"
                     /locus_tag="ACHK8E_002733"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP0344979.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31390..32682
                     /gene="ybaT"
                     /locus_tag="ACHK8E_002734"
     CDS             31390..32682
                     /gene="ybaT"
                     /locus_tag="ACHK8E_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0344980.1"
                     /translation="MMNTEGNNDNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            32713..32810
                     /locus_tag="ACHK8E_002735"
                     /pseudo
     CDS             32713..32810
                     /locus_tag="ACHK8E_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32807..33214
                     /gene="cueR"
                     /locus_tag="ACHK8E_002736"
     CDS             32807..33214
                     /gene="cueR"
                     /locus_tag="ACHK8E_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP0344981.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33215..33945)
                     /locus_tag="ACHK8E_002737"
                     /pseudo
     CDS             complement(33215..33945)
                     /locus_tag="ACHK8E_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34017..34223)
                     /locus_tag="ACHK8E_002738"
     CDS             complement(34017..34223)
                     /locus_tag="ACHK8E_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344982.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(34220..34642)
                     /locus_tag="ACHK8E_002739"
     CDS             complement(34220..34642)
                     /locus_tag="ACHK8E_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0344983.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(34728..35744)
                     /locus_tag="ACHK8E_002740"
     CDS             complement(34728..35744)
                     /locus_tag="ACHK8E_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0344984.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         8695 a         9328 c         9370 g         8373 t
ORIGIN      
        1 caatcagtat tttttatgaa ttttatacgt gtttaatact ggttttcgta tgagatcctg
       61 agttggtggt tcaatgactc cttaatgttc gtcggttatg cataaaaaag gccgcttgcg
      121 cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc
      181 ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat
      241 tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc
      301 gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa acgccaacga
      361 ggtcatcagg attgggcgta aacgcatccg caccgcatca agcgtcgctt caatcagacc
      421 tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc
      481 cgacaaacca atggttgtga gcaggcctac ctggaagtaa acgtcattgg tcaggccacg
      541 gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa
      601 cggaatcgac cagctctcgt acagcgcagc cagacacagg aacacgacaa tcaacgaaat
      661 cgcgtacagt gaaggcgcct ggttgccgga gagacgttcc tgataggaca ttcccgtcca
      721 gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc
      781 accggtactt ttacccggtg ccgcctggcc taagatttcc atagatggca ggccgttgta
      841 acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg agaacggcac
      901 catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta
      961 tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac
     1021 atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag aaacacccag
     1081 cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt
     1141 tggacgtacg ctggtcaaca tatcagggtg cttcgctgct tctgcaagca actggttacg
     1201 cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc
     1261 ggttgcggta cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt
     1321 gatttgcgag aaagcacgtg ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc
     1381 cggacgatcg gcccagtcct tcaaggaaac gaacgcaata cctgtattct gaccacgtcc
     1441 cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt ctttggtcag
     1501 atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg
     1561 cagctgaacc atggtcataa atacgccctg gtcttcatct ggcaagaagg agcttggcag
     1621 acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca ggtaacgccc
     1681 tgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat
     1741 gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt tggcaatcgg
     1801 tttcagcatg gtggcacaaa gagccggagt caggatcaac gccaccagta ccgacagcgc
     1861 cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag aaccgccaaa
     1921 gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca gagcgccctg
     1981 aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt ccgccataac
     2041 acgctcaacg ttttctacca caacgatggc gtcatccacc aacaggccga tggcgagcac
     2101 catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga cggcaaaggt
     2161 ccccagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga agttctgcag
     2221 gaacagatac ataaccagga acacgaggat gatcgcttcg actagcgttt taaccacttc
     2281 gtgaatcgag attttcacga acggcgtggt gtcgtacggg taaacaattt tcagacccga
     2341 cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg tatccagcgc
     2401 gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt taaactctgc
     2461 gatgatgtcg tagttctcac cacccagctc aattttcgcc acatcacgca gcagcacgcg
     2521 ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag aggtcagacg
     2581 cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg taccaccgag
     2641 ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat caaccggcgt
     2701 tagctggaat ttgttcagtt cattcgggtt catccagata cgcatcgcat actgtgaacc
     2761 gaacaactga acgtcaccca cgcccgacgt acggctgatg gcatctttca tattcgctgc
     2821 cacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac
     2881 catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgta cttcttgcgg
     2941 cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga tatccgcatc
     3001 agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac tgttagagga
     3061 catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg tcaccgtgtc
     3121 ctgcactgtt ttcgcatcag cgccagggta ggaggcggag atcgttactg ccggcggtgc
     3181 aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg ccaacatgat
     3241 gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag gcatgtctta
     3301 acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt gcggcttgct
     3361 ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc actttctgca
     3421 gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc
     3481 cgatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg ccaaccacca
     3541 gtacggtggc atcgccacgc ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt
     3601 ttggattaag cccttcttcc agacgtgccc gcacgaacat acccggcagc agagtgtgat
     3661 ccgggttcgg gaagatagcg cgtagggtga tagacccggt ggtctgatca acggtaacgt
     3721 cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg atcagcgaca
     3781 ctttggcttt gccgttttct tgtttcaacg tgccattcgc cagttcctgt ttcaggcgca
     3841 ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg
     3901 ccagcgtagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc gacttaccaa
     3961 tgcgaccgct aatcggcgag gtgactttgg tgtaagccag attgatccgc gcagtttcaa
     4021 cggcagcttt cgccgcagtg accgcagcat tcgcctgttg cgcatcagcc agagcctgat
     4081 cgtactcttg tttactgatg tactgagtac cgagcagttt ctgataacga ttcaccgtca
     4141 attgcgcgat attggctgca gcctgggctt tcgccaaatc acctttcgca ctgtcgtatg
     4201 ccgcctgata ggtcgcagga tcaatctgat agagagagac acctgcttcg atgtcgctac
     4261 cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact tctgcgatcc
     4321 ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca gttttgactg
     4381 ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg tcgtcacatc
     4441 ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta aaccctctgt
     4501 ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa
     4561 taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc acaaatgtat
     4621 gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac aagaagcgca
     4681 agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc agggggtatc
     4741 atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg caatctactg
     4801 gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag aatccaatat
     4861 tggtgaactc gagcttgagt atcaggcaaa attccctggc gatccactct cagtattaag
     4921 agagatatta attcatgttc ttgaatccac ggtgacagaa gaacggcgtc gattattgat
     4981 ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca
     5041 acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac attgtattga
     5101 agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta tgcgcggcta
     5161 tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc ttaaaaaaga
     5221 agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa
     5281 tcctgccact aacgaataac cttgaatctg actccaggat ttttcctgga cattttcgtc
     5341 gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc
     5401 ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa cgatcacggc
     5461 attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag aacacggcgt
     5521 ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag gcacaacttg
     5581 actcactgaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag caggatctga
     5641 cagatacatt agctaccctc gataaaatcg atcgcgtaaa agaagagaca gttcagctac
     5701 ggcaaaaggt tgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg ttaactgcac
     5761 ttagcgatgt cgataatgac gaagaaactc gcaaaattct gagtacgctt tcgttgcgcc
     5821 agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa aacgatctgg
     5881 cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga
     5941 tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg
     6001 gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag gctttgctga
     6061 atgccgagat tgaccagcag cgtaaaagcc tggaagggaa cacagtctta caggatacct
     6121 tgcaaaagca acgggattac gtgacggcga acagcgcccg tctggagcac cagttgcagc
     6181 tgttgcaaga agcagtaaac agcaagcgct tgactttaac cgaaaaaacg gcgcaggaag
     6241 ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaag caggaactgg
     6301 aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt aatcagttga
     6361 tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg gaacgcaata
     6421 ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc
     6481 aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc atcgcggatt
     6541 tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag agcgatgcgt
     6601 tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt
     6661 tattgcaagt ggttgatatg cgtcgcgaat tgctggatca gcttaacaaa cagttgggta
     6721 accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg agtgtgtcga
     6781 aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaatagtaac cgtccaatgg
     6841 actgggactg gatcaaagcg ttcccgcaaa gcctgagaga tgaatttaag tcgatgaaaa
     6901 tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc gctggtttgc
     6961 cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac
     7021 aaaaactggc ttccgctgtg ggttccctgc gtaacgacag ccagctcaac acaccaaaag
     7081 cgatccttat cgacctgatc cgtgcgctgc cggtgtgcct gattattctc gcggttggcc
     7141 tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc agtaaaaaac
     7201 tggcgatttt ctggctggtg tttggcctgt gctggaaagt gttggagaaa aacggcgttg
     7261 ccgtgcgtca ctttggtatg ccggaacagc agaccagcca ctggcgtcgg caaattgtcc
     7321 gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa ctttccccgc
     7381 tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacttg ctgctgattg
     7441 ccttcctggt gtggccgatg tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc
     7501 gactggtcac cattaccgta ctgtcgatta tcccgattgc gttgatggtg ctgaccgcca
     7561 cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc gtttatttgg
     7621 tgatcatctg gaacctgctg taccagacgg tattgcgcgg cttaagcgta gcggcgcggc
     7681 gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag
     7741 gtgctgaacc gccggaagaa cccaccattg cactggagca ggttaaccag cagacgctgc
     7801 gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca atttggtccg
     7861 atttgatcac cgtgttcagc tatctggaca gcatcacgct ctggcattac aacggcactg
     7921 aagctggtgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt gcgattatcg
     7981 cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttgctggaa gtgttggtgc
     8041 tctcgcgcct gaacatgcgc cagggcgcgt cgtatgccat tactaccatc cttaactaca
     8101 tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctcg tgggataaac
     8161 tccagtggct ggcggcagca ttatccgtag gtcttggttt tggtttacaa gagatcttcg
     8221 gtaacttcgt ttccggtttg atcattctgt tcgaacgtcc ggtgcgcata ggcgacacgg
     8281 taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg acaacgatta
     8341 ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga
     8401 tcaactggtc gttgactgac acgactacgc gtctggtgat ccgtctcggc gtagcttatg
     8461 gctccgatct ggaaaaagtg cgtaaagtgt tgctgaaggc ggcgactgag cacccaaggg
     8521 tgatgcacga accaatgccg gaagtcttct ttacggcatt tggtgccagc acgttggatc
     8581 atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc gatgagctga
     8641 accgtactat cgatcagttg tgccgtgaaa acgacatcaa cattgccttt aaccagcttg
     8701 aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtcaaacgc gactacaaag
     8761 gcgacgatcc gacgccagcg gtagggtaat aaccaaaggg ggcgacattt agttgccccg
     8821 agattactaa caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc
     8881 tacgaaaccg caagaattca atatattgca ggagcggtgt aggcctgata agcgtagcgc
     8941 atcaggcagt tttgcgtttg cccacaatct taggggacat ttagcggccc catttatttc
     9001 tcacttttcc gcctcatcat cgcgcgttaa tttcttttca taatcgcgtt ttacaatatc
     9061 cagcgcgcgc agcacggtgc gggctgggat ctgattttct tccagtagca caatcaaatc
     9121 gacggccagt ttgacatcgt caggggcatt ttccagtgac atactctctc cattgctagc
     9181 gggttaaacg cgctaacctg ttttcgattt tttccagcgc atggcggcag cgtgccaggc
     9241 gcgcttcata ggcttccact tcacgatgca gcgtctgctg ttccacgaga tcggtcgctc
     9301 gtgccagacg ggctctacgt tcggcaacca tctcacgtag ccgccgctca aaatcctgat
     9361 gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg tgcgctatcc cactcgcgta
     9421 gcgaccaggc tgtggcttca cgcgctatag cctccagttg cgccgccaga tgttctgcca
     9481 gccaggcgac ttgcggcagc tgttgctgct caaccgcatg acgaagcgca gccagattat
     9541 cgcccgcctc atcgagacaa gcctgcagtg ttgtcgcacg agtctgaaaa agatgcctgt
     9601 caaaacgggc gcttagcgtg gcaaattgcg ctaccggggc acaacgctga cgcagcgtag
     9661 cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga cctcaatgaa
     9721 aatgataatt gttatgctaa agtagcaact ctgtaagctg acaacactca atatgcaacg
     9781 aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt
     9841 accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg cccgttcttc
     9901 cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc tacgtttctg
     9961 gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct
    10021 cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt
    10081 aattctcgcc tgtttgcttt tttatatgtg gcgaattccg gtgattgatg aaaagcaaga
    10141 aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt acattctggc
    10201 gttttcgagc acaggcgcag gcggtcaaag gttaaacaac tgttactttt gatacgttta
    10261 aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc gactgcacag
    10321 cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa acccggcatt
    10381 cttttccgtg atgtcaccag cttactggaa gacccgaaag cttacgctct cagcatcgac
    10441 ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt
    10501 ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt accggtccgt
    10561 aaaccgggca aactgccgcg tgaaaccatc agtgaaactt acgacctgga atacggcacc
    10621 gatcagctgg aaatccacgt tgatgccatc aaaccgggcg acaaagttct ggtggtggac
    10681 gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg tcgtctgggt
    10741 ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg cgaacagcgt
    10801 ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca ttaattatcg
    10861 ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc tcgccgttct
    10921 gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt cagagcctgc
    10981 caatgagtta tcaggtctta gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg
    11041 gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt attcatcatg
    11101 cttatctttt ttccggtacc cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga
    11161 aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc
    11221 gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacta
    11281 aagtcgaaga tacccgcgac ctgctggata acgttcagta cgctccggcg cgtggtcgtt
    11341 tcaaagttta tctcatcgac gaagtgcata tgctgtcgcg tcacagcttt aacgcactgt
    11401 taaaaaccct tgaagagccg ccagagcacg ttaagttttt gctggcgacg accgatccgc
    11461 agaaattgcc ggtgacgatt ttgtcacgct gtctgcaatt tcatctcaag gcgctggatg
    11521 tcgagcaaat tcgccatcag cttgagcaca tcctcaacga agaacatatc gctcacgagc
    11581 cgcgggcgct gcaattgttg gcacgcgccg ctgaaggcag cctgcgagat gctttaagtc
    11641 tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg gtcagtgcga
    11701 tgctgggtac gcttgacgac gatcaggcgt tatcgctggt tgaagcgatg gtcgaggcca
    11761 acggcgagcg cgtaatggcg ctaattaatg aagctgctgc ccgtggtatc gagtgggaag
    11821 cgttgctggt ggaaatgctc ggtctgttgc atcgtattgc gatggtacaa ctttcgcctg
    11881 ctgctcttgg caacgacatg gccgccattg agctgcggat gcgtgaactg gcgcgcacca
    11941 taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc aaagaattac
    12001 cgtatgcgcc agaccgccgt atgggtgttg agatgacgct gctgcgcgcg ctggcgttcc
    12061 atccgcgtat gcctctgcct gagccagaag tgccacgcca gtcctttgcg cccgttgcgc
    12121 caacggcagt aatgacgcca acacaggtgc cgccgcaacc gcaatcagcg ccgcagcagg
    12181 caccgactgt accgctcccg gaaaccacca gccaggtgct ggcggcgcgc cagcagttgc
    12241 agcgcgtgca gggagcaacc aaagcaaaaa agagtgaacc ggcagccgct acccgcgcgc
    12301 ggccggtgaa taacgctgcg ctggaaagac tggcttcggt caccgatcgc gttcaggcgc
    12361 gcccggtgcc atcggcgctg gaaaaagcgc cagccaaaaa agaagcgtat cgctggaagg
    12421 cgaccactcc agtgatgcag caaaaagaag tggtcgccac gccgaaggcg ctgaaaaaag
    12481 cgctggaaca tgaaaaaacg ccggaactgg cggcgaagct ggcggcagaa gccattgagc
    12541 gcgacccgtg ggcggctcag gtgagtcaac tttcgctacc aaaactggtc gaacaggtgg
    12601 cgttaaatgc ctggaaagag gagagcgaca actcagtatg tctgcatttg cgctcctctc
    12661 agcggcattt gaacaaccgc ggcgcacagc aaaaactggc tgaagcgttg agcacgttaa
    12721 aaggttcaac ggttgaactg actatcgttg aagatgataa tcccgcggtg cgtacgccgc
    12781 tggaatggcg tcaggcgata tacgaagaaa aacttgcgca ggcgcgcgag tccattattg
    12841 cggataataa tattcagacc ctgcgtcgat tcttcgatgc ggagctggat gaagaaagta
    12901 tccgccccat ttgatcgtaa gcacagctta cgttcgtcat ccttaacgtg attgagagag
    12961 aaacctatgt ttggtaaagg cggtctgggt aacctgatga agcaagccca gcagatgcaa
    13021 gaaaaaatgc agaaaatgca ggaagagatc gcgcagctgg aagtcaccgg cgaatctggc
    13081 gcaggtctgg taaaagtgac catcaacggt gcacacaact gccgtcgcgt agagatcgac
    13141 ccgagcctgc tggaagacga caaagagatg ctggaagacc tggtggctgc agcattcaac
    13201 gacgcagcac gtcgtattga agaaacgcag aaagaaaaaa tggcctctgt atcctccgga
    13261 atgcagctgc cgcctggctt taagatgccg ttctgatgca aaccagcccg ctgttaacac
    13321 agcttatgga agcactgcgc tgtctgccgg gcgttggccc gaagtcggcg cagcgtatgg
    13381 cgttcacgct gcttcagcgc gatcgtagcg gcgggatgcg tctggcgcag gcgctcaccc
    13441 gggcgatgtc ggaaatcggc cactgcgccg attgccgcac cttcaccgaa caggaagtct
    13501 gtaacatctg ttcgaatcca cgtcgtcagg aaaacggtca aatctgcgtg gtggagagtc
    13561 cggcggacat ttacgccatt gagcagacgg ggcagttttc aggtcgttat tttgtgttga
    13621 tgggacacct gtcaccgctg gacggcatcg gtcctgatga tatcgggctt gatcgtctgg
    13681 aacagcgtct ggcagaggaa aaaatcactg aagtgatcct cgccaccaac cccacggttg
    13741 aaggtgaagc taccgctaac tacattgccg agctttgtgc gcaatatgac gtggaagcca
    13801 gccgaatcgc tcatggcgta ccggttggcg gcgaactgga aatggtcgat ggcaccacgc
    13861 tgtcacattc ccttgccggg cgtcataaga ttcgttttta agcaaacgag agcaggatca
    13921 cctgctctcg cttgaaatta ttctcccttg tccccatctc tccctcatcc tgtttttaac
    13981 cttaaaatgg cattattgag gtagacctac atgaaaggac aagaaactcg tggttttcag
    14041 tcagaagtga aacagcttct gcacctgatg atccattctc tctattccaa taaagaaatc
    14101 ttcctgcgtg agcttatctc taacgcctcc gatgcggcgg acaagctgcg tttccgcgcg
    14161 ctttctaacc cggacctgta cgaaggtgat ggtgaactac gcgttcgtgt ctctttcgat
    14221 aaagacaagc gcacgctgac catctccgat aacggcgtgg ggatgacccg cgacgaagtg
    14281 attgaccatc tggggactat cgctaaatcc ggcaccaaat cattcctcga atccctgggt
    14341 tctgaccagg cgaaagacag ccagctgatc ggtcagtttg gtgttggttt ctactctgcg
    14401 tttatcgtgg ctgacaaagt gaccgtgcgt actcgcgcag caggcgaaaa accagaaaat
    14461 ggcgtcttct gggaatcggc tggtgaaggt gaatacactg ttgccgacat caccaaagaa
    14521 gatcgtggta ctgaaatcac cctgcatctg cgtgaaggcg aagacgagtt cctcgatgac
    14581 tggcgtgtgc gttccatcat cagcaaatat tccgaccata tcgcgctgcc ggtagagatc
    14641 gaaaaacgcg aagagaaaga cggcgaaacc gttatctcct gggagaaaat caacaaagcg
    14701 caggcgctgt ggactcgtaa caagtcggaa atcaccgatg aagagtacaa agagttctac
    14761 aaacacatcg cccacgactt taacgatccg ctgacctgga gccacaaccg cgttgaaggt
    14821 aagcaggagt acaccagcct gctgtacatt ccgtctcagg caccgtggga tatgtggaac
    14881 cgcgatcata aacacggtct gaaactgtat gttcagcgtg tgttcatcat ggacgacgca
    14941 gaacagttca tgccgaacta tctgcgcttc gtgcgtggtc tgattgactc cagcgatctg
    15001 ccgctgaacg tttcccgtga aatcctccag gacagcacgg taacgcgtaa cctgcgcaat
    15061 gcgctgacca agcgtgtgct gcaaatgctg gaaaaactgg cgaaagacga cgcggaaaaa
    15121 taccagacct tttggcaaca gtttggcctg gtactgaaag aaggtccggc ggaagatttc
    15181 gctaaccagg aagcgatcgc caaactgctg cggtttgcgt ctacccatac cgattcttct
    15241 gcgcagaccg tgtctctgga agactacgtt tcccgcatga aagaagggca ggagaaaatc
    15301 tactacatca ctgcagacag ctacgcggca gcgaagagca gcccgcacct ggaactgctg
    15361 cgtaagaaag gcatcgaagt tctgctgctt tccgaccgca tcgatgagtg gatgatgaac
    15421 tatctgactg agttcgacgg taaaccgttc cagtctgtgt ctaaagttga cgagtcgctg
    15481 gaaaaactgg ctgacgaagt tgatgagagc gcgaaagaag cggagaaagc actgactccg
    15541 tttatcgacc gtgtgaaagc cctgctcggc gagcgcgtga aagatgtccg tctgactcac
    15601 cgtctgaccg atacgccagc gatcgtttcg accgacgcgg acgaaatgag cactcagatg
    15661 gcgaaactgt tcgctgcggc gggccagaaa gtgccagaag tgaaatacat cttcgaactg
    15721 aacccggatc acgtactggt gaaacgtgcg gcagatactg aagatgaagc caagttcagc
    15781 gagtgggtag aactgctgct ggatcaggcg ctgctggcag aacgcggcac gctggaagat
    15841 ccgaacctgt ttattcgtcg tatgaaccag ctgctggttt cctgatgtca tgccggatga
    15901 cctttgtgtc atccggcatt tttcttttca tcatctgcac tttccgcaaa ttatctcgcc
    15961 attaaccgtt tcagccccag gtgcctttct tgaggcaatc gcctgttggt ggtatcgttt
    16021 atcgcttttt caaaaaattc gacacatttt aaggggattt tcgcaatgcg tatcattctg
    16081 cttggcgctc cgggcgcggg gaaagggact caggctcagt tcatcatgga gaaatatggt
    16141 attccgcaaa tctccactgg cgatatgctg cgtgctgcgg tcaaatctgg ctccgagctg
    16201 ggtaaacaag caaaagacat tatggatgct ggcaaactgg tcaccgacga actggtgatc
    16261 gcgctggtta aagagcgcat tgctcaggaa gactgccgca acggtttcct gttggacggc
    16321 ttcccgcgta ccattccgca ggcagacgcg atgaaagaag cgggcatcaa tgttgattac
    16381 gttctggaat tcgacgtacc ggacgaactg attgttgacc gtatcgtagg ccgccgcgtt
    16441 cacgcgccgt ctggtcgtgt ttatcacgtt aaattcaatc cgccgaaagt agaaggcaaa
    16501 gacgacgtta ccggtgaaga gctgactacc cgtaaagatg atcaggaaga gaccgtacgt
    16561 aaacgtctgg ttgaatacca tcagatgact gcaccgctga tcggctacta ctctaaagaa
    16621 gcggaagcgg gtaacaccaa atacgcgaaa gttgacggca ccaagccggt ggctgaagtt
    16681 cgcgcagctc tggaaaaaat tctcggctaa tcaagccgca gcagatctgc tttcgggcag
    16741 gtctgctcat cctcctcagg caatacctct cacagcaatt agttcttctt cttcactttt
    16801 ccgctacaat tatcaacaag ttgaatcgat aagaggcggt aatgcgtcag acaaaaaccg
    16861 gtatcctgct ggcaaacctg ggtacgcccg atgcccccac acctgaagcg gtaaaacgct
    16921 atctgaaaca atttttaagc gacagacgtg tggttgatac ctcacggttg ttatggtggc
    16981 cattgctgcg cggcgtgatt ttgccactgc gctcgccgcg tgtggcgaag ctgtatgcct
    17041 ctgtctggat ggaaggtggc tcgccgctga tggtttacag ccgccagcaa cagcaggcgc
    17101 tggcacaacg tttaccggag acgcccgtag cgctgggaat gagctacggc tcgccatcac
    17161 tggaaagcgc cgtagatgaa ctactggcag agcatgtaga tcatattgtg gtgctgccgc
    17221 tttatccgca attctcctgt tcaacggtcg gtgcggtatg ggatgaactg gcacgcattc
    17281 tggcgcgcaa acgtagcatt ccggggatat cgtttattcg tgattacgcc gataaccacg
    17341 attatattaa tgcactggcg aacagcgtac gtgcttcttt tgccaaacat ggcgaaccgg
    17401 atctgctgtt gctctcttat catggcattc cccagcgtta tgcagatgaa ggcgatgatt
    17461 acccgcaacg ttgtcgcaca acgactcgcg aactggcttc cgcactgggg atggcaccgg
    17521 aaaaagtgat gatgaccttt cagtcgcgct ttgggcggga accctggctg atgccttata
    17581 ccgacgaaac gctgaaaatg ctcggagaaa aaggtgtagg tcatattcag gtgatgtgcc
    17641 cgggctttgc tgcggattgt ctggagacgc tggaagagat tgccgagcaa aaccgtgagg
    17701 tcttcctcgg tgcgggcggg aaaaaatacg aatatattcc ggcacttaat gccacgccgg
    17761 aacatattga aatgatggct aatcttgttg ccgcgtatcg ctaaagctga gcggtaaaga
    17821 actgagcgcc gtcgcgaaga gcctcgtcgg cggttttcat catccgtgaa taatgcaaaa
    17881 aggcgtgcag cgtgcctggg tagagtttga actcacaggg ctgctgatgc gccgctaacg
    17941 tctggtaaag cagacggctg tcatccagca gcggatcgaa ctccgccccg gcaataaaac
    18001 agggcggaac ttcgcgagtg agatcattat taaacaggca gtaatacggc gactcgcggt
    18061 ccgcgtcgtt gcttaaatac gcctcttcat acatctgtaa atcctgttgc gttaagccat
    18121 cccagacacc gcccaacaga cgacgagtca cggaatcccg taatccgtaa agcccatacc
    18181 acagcaaaac gcccgcaact ttaccgcaat cgatctgttt atcacgcaac cacaacgcac
    18241 tggcgagcgc caacatggca cctgcggaat caccggtaaa gccaatgcgg gacatattga
    18301 tttgataatc ttccgcctgc tggtggaaat aacaacaggc agccacaatt tcctctattg
    18361 cttgcggaaa acgcgcttca ggtgaaaggg tgtaatcaat gccaatcacc gtacattggc
    18421 tgtagcttgc cagcaggcgc atgatgcgat cgtgggtatc aagattgccg agaataaaac
    18481 cgccgccatg caaataaaat agcgtcgccg ggctatctgg ctgcggacaa aagagacgtg
    18541 tttccacctg cccatatttt gttggaacca tgtaagctct ggttgccatt tctggagcgc
    18601 ccgcattcca gaatcggcgc tcaagcgtgt aatactgtcg ttgctcagca atcgttcccg
    18661 ttgcgggcca gggcggtaaa tccggctgaa gagtattcac aacggtcttc atttcagcag
    18721 aaataaggtc cagaacgggt aatttgtttt ccggcttcat gcaaaaactc ctttcaaatt
    18781 acgtcattgt aaggaaccac tgccatgaaa atgcgatccc gcctgctgat attgaaactg
    18841 gctgcgtctc gcgcgctccc gtcagattgt gttaacattc gccgctcagt taaccacccg
    18901 taaaaacaac catgaaattt cccggcaaac gtaaatccaa acattatttt cccgtaaacg
    18961 cacgcgatcc gctgcttcag caattccagc cagaaaacga aaccagcgct gcctgggtag
    19021 tgggtatcga tcaaacgctg gtcgatattg aagcgaaagt ggatgatgaa tttattgagc
    19081 gttatggatt aagcgccggg cattcactgg tgattgagga tgacgtagcc gaagcgcttt
    19141 atcaggaact aaaacagaaa aacctgatta cccatcagtt tgcgggtggc accattggta
    19201 acaccatgca caactactcg gtgctcgcgg acgaccgttc ggtgctgctg ggcgtcatgt
    19261 gcagcaatat tgaaattggc agctatgcct atcgttacct gtgtaacact tccagccgta
    19321 ccgatcttaa ctatctacaa ggcgtggatg gcccgattgg tcgttgcttt acgctgattg
    19381 gcgagtccgg ggaacgtacc tttgctatca gccccggcca catgaaccag ctgcgggctg
    19441 aaagtattcc ggaagatgtg attgccggag cctcggctct ggttctcacc tcttatctgg
    19501 tgcgttgcaa gccgggtgaa cccatgccgg aagcaaccat gaaagctatt gagtacgcga
    19561 agaaatataa cgtaccggtg gtgctgacgc tgggcaccaa gtttgtcatt gccgagaatc
    19621 cgcagtggtg gcagcaattc ctcaaagacc acgtctctat ccttgcgatg aacgaagatg
    19681 aagccgaagc gttgaccgga gaaagcgatc cgttgttggc atctgacaag gcgctggact
    19741 gggttgatct ggtgctgtgt accgcagggc caatcggctt gtatatggcg ggctttaccg
    19801 aagacgaagc gaaacgtaaa acccagcatc cgctgctgcc gggtgctata gcggaattta
    19861 accagtatga gtttagccgc gccatgcgcc acaaggattg ccagaatccg ctgcgtgtct
    19921 attcgcacat tgcgccgtac atgggcgggc cggaaaaaat catgaacact aatggagcag
    19981 gggatggcgc actggcagcg ttgctgcatg acattaccgc caacagctac caccgtagca
    20041 acgtaccaaa ctccagcaaa cataaattca cctggttaac ttattcatcg ttagcgcagg
    20101 tgtgtaaata tgctaaccgt gtgagctatc aggtactgaa ccagcattca cctcgtttaa
    20161 cgcgcggctt gccggagcgt gaagacagcc tggaagagtc ttactgggat cgttaagtta
    20221 tcgaccagac cggacgacga tggcatccgt aggccggata agatgcgtca gcatcgcatc
    20281 cggcaatgga atcaatgttt taccccgtca ccacctcacc cgccggtggc gtttccagca
    20341 gttccagcat agtacgagca atttcacgct cacccatcac tacctgattc gcaccacgtt
    20401 cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca atatccggat
    20461 ttttcgcgcg ggcagatgcc acaatctcgc ccgcttcata accgttggga atcgtcagga
    20521 tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc gccgcattgc
    20581 ccaatactgc gcggaccccg cgctctcgca gttcatcaac acgggttcgt gacgtctcaa
    20641 tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg cctacacgac
    20701 cgtaacctac cagtagcgca tggttgcaaa tatccactgg gatctgcttc tcttcttcga
    20761 tagcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat ttctccagca
    20821 gcgcaaacag gaccgggttg agcataatcg acaaaatcgc ccctgccagt accaggttct
    20881 gcccggcttg cggcaataga tccagcgcca tgcccagtcc cgccaggata aacgcgaact
    20941 caccaatttg cgccaggctg gcggcgatag ttaatgccgt acgttgggag tgaccaaaca
    21001 gtcgcaccag gaaaaacgcg gctaacgact taccaaacag aataatcgcc agcgtcgcca
    21061 gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg acggagacaa
    21121 aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca cggtgactca
    21181 gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag gagacatcaa
    21241 acagctccac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac agggtaaaca
    21301 gctcacggga accggttgct gcgctacgtg ccataatcca cggcaccaga cggcgaccta
    21361 ccagcatcat aatggcgata aatgcgatca ccttgccgat ggtgatcccc atatcgacag
    21421 caagagtggc aaagcccaca tcgccttgtt ccatcattcc tgccactgcg ggcagcaaca
    21481 ccagcgtcag aaccatgacc aggtcttcca caatcaacca accgatggcg atttgcccac
    21541 gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg
    21601 tggaaaggca taagccgaac acgataccgg tcattaacga ccagcccagc acggcggaga
    21661 gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcaccggga atggcgatgg
    21721 cctttaccgc catcaaatcc ttcagcgaaa agtgcagacc cacgccaaac atcaacagaa
    21781 tgacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag cccggagtga
    21841 atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata cgtagtttat
    21901 tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg gtgataagcg
    21961 gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt ttggccggga
    22021 aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttta tgaataattg
    22081 ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga aaacagaagg
    22141 cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt
    22201 ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc aacatcccca
    22261 atagtggcag gaaagcacag attttataga ctaactcgat gctggtgtga tcggcgataa
    22321 gccccagaac tgccgctccc agacctccca tgccaaaagc aaaaccgaaa aagagtccag
    22381 aaaccatacc gatacgtccc ggaagtagct cctgagcgta gaccagaatg gcagagaatg
    22441 ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc aggctggcgt
    22501 agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc acatatttcc
    22561 gtccaatttt atcccctaca ggcccgccaa tcaccgtacc tgccgcaacg gcaaacagga
    22621 aggcaaacaa atgaagctga gcattctgga tagataatcc gaatttttgc atcagataaa
    22681 aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg aggattaaca
    22741 gaatgctgac cgccagaaca actttattgc gcggcagtgg attgataatc gtcgctttgg
    22801 gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc gccaacacca
    22861 cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca taaggcgcga
    22921 taatcaccgc cgccagcaag gggcccaggg aactgccaaa gttgccgccg acctgaaaga
    22981 tagattgcgc caggccatga cgcccgccgg aagccatacg ggccacgcga gaagattccg
    23041 gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc cagcagaact gcgccgaaac
    23101 tgcccgccag cgccagcagc accagaccac ttaaggtaaa gcacatgcca attggcaacg
    23161 accacggcat cggatattta tcggtccagt agccgacaac tggttgcagt agcgaagagg
    23221 cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa aactctgact
    23281 gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc agatgtgaga
    23341 ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc
    23401 ccgccacagg ctgggtttgt tcactcattg ccataggaaa gtcacttttt cagggttgcg
    23461 atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta tgcgccgcct
    23521 gaaggaagtc tcaacgctga atacagaatt tctaatctgg atacagattt atcttcaccg
    23581 gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga aatcaggtca
    23641 gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcactgtta accacattta
    23701 cactggcgag tgaagctgct ctggcgtatg agcaggataa aacctacaaa attacagttc
    23761 tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa tatggtctgg
    23821 cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg
    23881 tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtccgac ttacaggatg
    23941 ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg atcggtaatc
    24001 atgaatttga taacccgctc accgtattac gccagcagga aaagtgggct aagttcccgt
    24061 tgctttccgc caatatctac cagaaaagta ccggcgagcg cctgtttaaa ccatgggcgc
    24121 tgtttaagcg tcaggatctg aaaattgccg ttattggcct gacgacggat gacacagcca
    24181 aaattggtaa tccggaatat ttcactgata tcgaatttcg taagcccgct gatgaagcga
    24241 agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc gcggcgaccc
    24301 atatggggca ttacgacaat ggtgagcacg gctctaacgc accgggcgat gtggagatgg
    24361 cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggccactcg caagctccgg
    24421 tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg ccatgcaaac
    24481 cggatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa tacgtgggac
    24541 gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt taactaccag ctgattccgg
    24601 tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg ctgtacaccc
    24661 ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag aacaaaggca
    24721 aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc gatcgtgaca
    24781 aagtacgttt tgtacagacc aatatggggc ggttgattat ggcagcacaa atggatcgca
    24841 ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc gaagcaggtg
    24901 atatcagcta taaaaacgta ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg
    24961 acatgaccgg taaagaggtg atcgattatt tgaccgccgt cgcgcagatg aagccagatt
    25021 caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggt aaactgaaca
    25081 acctgaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgcatg gcgacattaa
    25141 acttcaatgc caccggcggt gatggctatc cgcgccttga taacaaagcg ggctatgtga
    25201 ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg
    25261 atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa agtgccggat
    25321 gttcgcatcc ggcacaatgc ttaatcgcga cgggcgatat cagcaaattt ggcatcgagg
    25381 atctttgcca gatcgcctgc cgccagttcg atatccagcc cgcgcttgcc gccggaaaca
    25441 taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg ttttttctgc
    25501 cccagtgggc taatcccccc taccagatac cccgtcgaac gctgcgcgac catcggatcg
    25561 gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc cagttgacct
    25621 gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc gaccagcagc
    25681 gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc gccaaaattg
    25741 gtttcagccg gatcgtgctc gtaggtgtgg atttgaaacg aaatcttgtt tttttcgagt
    25801 aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat
    25861 acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg caggatggtg
    25921 agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata ataaaaaaat
    25981 gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc aacatctgcg
    26041 gcaaattccc ttctccatac aggtgtagtg caccaaccgc gaccacatag cgccccggcg
    26101 gcatggcgca taatttgtcc cgccaggcga gatttcgctg atgcatcagc acatcgtaca
    26161 gcgactgact gaacgtatta ggcagcgtta tttcattatt ttgcggcggc gcattcagcc
    26221 accagctcat catttgttgc agcaaccgtg cgttggtgtg ccagtgggtc agcgtatcgt
    26281 ccagcagcgc cagtccgttg tcagggagct ggagcaacat ggcaatctgg ttttcagccc
    26341 cttccagttc aatcacgggt ttatgttgtt gcttagccgc ctgcaatagc tggtaatcga
    26401 taccgtattc tgcccgcagc cccagttttt gcgcctgcgt cgcctgaaga accatcgcga
    26461 tttgccacag cggttgagta gaaaagagcg acggagaaat gcccatctcc tggctaatat
    26521 gctgcaggtt ttggagttgc tcctcgctaa tgcgcttttc cagggcctcg caggcaggca
    26581 aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc gcatcggcgt
    26641 ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta cccatatgaa
    26701 tactgccgat cagatgaaaa tggcgattgc cgggaagaga gatatcgata gcaggccagg
    26761 tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac agatccatac
    26821 gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggcgcaa atggagttga
    26881 ttgttgcact tgcgcacagt gcttgtgtgc attacaatga ttaaataatg gacaaggaga
    26941 tgagagagga atggctcaaa agaaaaatat aaggagtttt cgtgatgcct ggttggctga
    27001 tttttttgtc cattcaacgc cacatcgaaa gatccctgct gaaatccata ccacgttatc
    27061 acgaaagttg gacatcatta atgctgccac ttctcatcgg gatttacgct cccctccggg
    27121 gaaccgatac gaagagttat ccgggaaatt acaggaatac tcctccatta gagtgaataa
    27181 acagtaccga ctgattttta aatgggttaa tggcaaggcc gaagaattgt ttttagaccc
    27241 acacaactac taaaacatag ctgtcgtatt gacgagaaag gtaaaactat gaaacaggca
    27301 acaagaaaac cgacgacacc cggagatatt cttctctatg aatatctgga accgctcgat
    27361 ttgaaaatca atgagttagc agagttgctg catgttcatc gtaatagcgt cagtgcactg
    27421 atcaataaca atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat
    27481 accacagtcg atttttggct aaacctccag gcggcagttg atctttggga agttgaaaac
    27541 aacatgcgta cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc
    27601 cgtgaagagc gtgcaaaaaa ggtcgcgtag aataatttcc ctataacgga tgcgccaccc
    27661 taaagcagtg catccgcaat gatgtactta ttccttcggt ttaaaccgca gcaaccggtt
    27721 ggcgttactc actacggtaa tcgacgagag cgccattgcc gctccggcga ccaccgggtt
    27781 aagcagcgta ccggtgaacg gccacaaaat accggcggcg accggaatac caatgctgtt
    27841 gtagataaac gcaccgagca ggttctgctt catattgcgc agcgttgcgc gggaaatcgc
    27901 gagcgcatcc gcaacaccca tcaggctatg gcgcatcagg gtaatcgccg cggtttcaat
    27961 ggcaacatca ctgccgccac ccattgcaat gccgacatcc gcctgagcca gcgctggcgc
    28021 gtcgttaatg ccatcgccca ccattgccac ctgacgtcct tcactttgca gacgtttgat
    28081 cgcttcggct ttaccgtccg gcagcacccc ggctatcacc tcatcaatcc ctgcttcttt
    28141 ggcgatcgca ttggcggtgg ttgggttatc cccggttagc atcaccagac gatatcccgc
    28201 tttatgcagg cgttgcagcg ccgccacgct atcactacgc aatggatcgc gtacagccag
    28261 cagggctacc gctttcccgt caaccgccag cagcacaggc gttgcccctt gcgatgcctg
    28321 agctgaaata tctgcttcaa tcgctttagt atcaacttgc tggtcattta acagcgcctg
    28381 attgcccagc aataacgcat gaccttcagc ttcaccgctc acgcccagcc cgcgcaatgt
    28441 gcggaaaccg ttgacctgcg gcagctgcat atcgcttgct ttatcgagga tcgcccgtgc
    28501 cagcggatgg ctggaacctt gttccagtgc cgccgccaga cgcagcgcct gcgcttcgtc
    28561 aaaatcagca aatgttttta ctgcgacaac ctgcggcttc ccttcggtca gcgtcccggt
    28621 tttatcgaac accacggtgt cgagcgtact ggcgcgttgc agcgcgtcgg cgtcccgcac
    28681 cagcacgcca aactcagccg cccgcccgac gcccgaaata atcgacatcg gcgtcgccag
    28741 tcccagcgca cacggacagg cgataatcag caccgtggtg gcaatcacca gggtataaac
    28801 aatttgcggt gccgggccaa agaaatacca gattgccgca ctgaccagcg caatgactac
    28861 caccaccggc acaaacacag cggagatttt atccgccagt tgaccgattt ctggcttgct
    28921 gctctgggcc tgtcgcacca tgcgaatgat ccgtgacagc gtagtatggc tgccgaccgc
    28981 actggcgcga aacagcacac tgccgtcctg taccactgtc ccggcatgga cgctatcgcc
    29041 ttcgcctttt tgctgcggga ttggttcgcc cgtcagcatc gcttcatcca gccacgcttc
    29101 gccctgggta atttcaccat ctaccggcac ccgatcaccc gtcgtcaggc gcagcaacat
    29161 acctggctga acatctgcca gcggcacgtt tttttcacct tcgttggtga ccaggcgcgc
    29221 cgtcggtggg gttaaatcga gtaatttctc cagcgcctta gaagaacgct gacgtgcgcg
    29281 cgcttccagc atatgaccga gattaatcag accgataatc atcgcactgg cttcatagta
    29341 aagatgtcgc gcttccatcg ggaaccactg cggccacagg ttgacgctca ttgaatagag
    29401 ccacgccacg cccgtaccca gcgccaccag cgtatccatc gtcgctgcac cgttcagcag
    29461 gcttttccat gcgctgcggt aaaaatggcc gccggcgaaa accattaccg ccagggttat
    29521 caggccgata accaaccaca ggctgcggtt gtcggcggtg accatcatgt tatcgccgat
    29581 catcccccag accatcaccg ggatacccac cgccagtgcg acaatcgcct gccagcggaa
    29641 gcgcttcatc gtagcgacgg cggtttcttg ctggcgctcg cggcgtttag cgtcatcttc
    29701 aatcgcttcc gcgccgtagc ccgctttttc caccgcctgc actaaatctt gtggggaggc
    29761 actgcccatc accagcgcag tacgctccgc caggtttacc cgtgcctgag tgacgcccgg
    29821 tacgctttgc aacgcatttt gtacgcgggt gacacagctg gcgcagctca tgccgctcag
    29881 cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc ggaagcgcct cagaaaccgc
    29941 tgtcagtgct tccgacggga ttgatgactc cgccagcggt ttagcctttg ggtggcttac
    30001 agatgcgtca taacccgctt gtttgatggt ttcgatcagc tgttctgcac tggcagtccc
    30061 ggtaacgtgc gcttcagtga cagacacatc cgcctgctca acatccgggc gctgttcgag
    30121 actttctttc acgcgtttaa cgcagtgacc gcaggacagg ccgtccaggg tcaggtcgat
    30181 agtttgtgac ataaaacact ccttttaaga cagttttgac tggctgtgat aaaggttaaa
    30241 ccttccagca aggggaaggt caagaaatta ataaaccagg cgggtaaaag tacgtaaaga
    30301 ttaaaaaatc ggctcgattt gcatcaggat tagacattta tctctttgtt ttcctgtagt
    30361 taagttgcgg gtgctaagtt aaatcaggat gcctgaaaat tggcaccggg gtgaggaatt
    30421 acctcccgca tctataaaaa ggagttaaca aaagatgtta gatgcaaaca aattacagca
    30481 ggcagtggat caggcttaca cccaatttca ctcacttaac ggcggacaaa atgccgacta
    30541 cattcccttt ctggcgaatg tacctggtca actggcagca gtggctattg tgacctgcga
    30601 tggcaacatc tatcgcgcgg gtgatagtga ttaccgcttt gcgctggaat ccatctcgaa
    30661 agtctgtacg ttagcccttg cgttagaaga tgtcggcccg caggcggtac aggacaaaat
    30721 tggcgctgac ccgaccggat tgccctttaa ctcagttatc gccttagagt tgcatggcgg
    30781 caaaccgctg tcgccactgg taaatgctgg cgctattgcc accaccagcc tgattaacgc
    30841 tgaaaatact gaacaacgct ggcagcgaat tttacatatc caacaacaac tggctggtga
    30901 gcaggtagcg ctctctgacg aagtcaacca gtcggaacaa acaaccaact tccataaccg
    30961 ggccattgcc tggctgctgt actccgccgg atatctctat tgtgatgcaa tggaagcctg
    31021 tgacgtgtac acccgtcagt gctccacgct catcaatact gttgaactgg caacgcttgg
    31081 cgcgacgctg gcggcgggtg gtgtgaatcc gctgacgcat aaacgcgttc ttcaggccga
    31141 caacgtgccg tacattctgg ctgaaatgat gatggaaggg ctgtatggtc gctccggtga
    31201 ctgggcgtat cgcgttggtt taccgggcaa aagcggtgta ggtggcggta ttctggcggt
    31261 cgtccctggc gtgatgggaa ttgccgcgtt ctcaccaccg ctggacgaag aaggcaacag
    31321 tgttcgcggt caaaaaatgg tggcatcggt cgctaagcaa ctcggctata acgtgtttaa
    31381 gggctgatca tgatgaacac ggaaggtaat aacgataaca aacccctcgg tctatggaac
    31441 gtcgtttcca tcggtattgg ggcaatggtg ggggcgggga tcttcgcgct gctggggcag
    31501 gctgcgttgc taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg
    31561 atgttttccg gttatgccta tgcgcgcctg ggggcgagct atcccagcaa tggcggtatt
    31621 atcgacttct ttcgtcgcgg attaggcaac ggcgtctttt cgctggcact ctcgttattg
    31681 tacctgttga cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc
    31741 gtgcagtttt tgcatgaagg cagtcaggag gagcacctta tattgctcta cgcgttgggg
    31801 atcattgcgg tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggaa
    31861 gtgatcctcg tcggcattaa aatgatgatc ctgttattgc tgatcattgc cggtgtctgg
    31921 tcgctgcaac cggcgcatat ttccgtctct gcgcccccca gctccggtgc gttcttctcc
    31981 tgtatcggga taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat
    32041 aaagtgaaag atccgcaggt cattatgcca cgggcgtttc tggtggcgat tggcgttacc
    32101 acgttgcttt atatctcgct ggcgctggtt ttgcttagcg atgtatcggc attagagtta
    32161 gaaaaatatg ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg gcatgtgggt
    32221 tatgtgatcg tcgtcatcgg cgctttactg gcgacggctt cagccataaa cgcgaacctg
    32281 ttcgccgtgt ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg
    32341 aataaatccc tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt gctgattatg
    32401 ctgatgacgg cggcgctgaa tttaggctca ctcgccagcg ttgccagcgc cacctttttg
    32461 atctgctatc tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg
    32521 ttgccgattc ttatcgttgg tacgttggtt atgttgttgg tgatcgttgg ctttatctac
    32581 agtctctggt cccagggcag ccgtgcgctg atatggatta ttggcgcact cttactcagc
    32641 cttattgtgg caatggtcat gaagcgcaat aaaactgtat aacatctctc tgtgcgcagt
    32701 acctcctgta ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat
    32761 atctgttgaa accctttaac aaagcacagg aggcgttgcg cgaacgatga acattagcga
    32821 tgtagcaaaa attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct
    32881 ggtgacgccg ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa
    32941 cgaactgacc ttactgcgcc aggcacgcca ggtgggcttt aacctggagg agagcggcga
    33001 actggtgaat ctgtttaacg atccgcagcg tcacagcgcc gacgtcaaac gtcgtacgct
    33061 ggagaaagtg gcggagatcg aacagcatat cgaagaactg caatccatgc gtaaccagct
    33121 gctggcgctg gcgaatgcct gccccggtga tgacagcgcc gactgcccga ttatcgaaaa
    33181 tctctccggc tgctgtcatc atcgggcggg gtgattaagg cttgcgaaaa tagtcgtcca
    33241 ttattttcgt cagctccgga cgttccccgt catcattgat ggcaaaatcc agaaaaaaag
    33301 catctgcttg cggcgttcgg ttttgcgcca tcaaccggaa ggtgaggggg cctgaccagg
    33361 tttcggatgg gctgatgtgc ttatcagggc cgaaactctc ccatgcgttg ggcggtaatg
    33421 ttttgcacgc ggtgagtttt ggtatgtggt attgcagctc tgttttgtgc agacattccc
    33481 cgccaggaaa taccagggtt tgttgctcat cattaaaatg ggttgccgtg ctgagcacca
    33541 gtgttttgag cggtcgccct tccagcgcgg cggcagtagc cttatggtgt ccgtcgagca
    33601 gaacgcagag ataggaatca gggagatacc aggccagagc gcgggcgatt ccagttttcg
    33661 agagcagaca aaaaccgggc ggctccctgt tcaccctccg caaaaccgct gtagacaaat
    33721 tccgcacagg tagggcaacc agcctcggac cagaaacgta ttaacgggac atccttgcca
    33781 gcagagcgaa tggataagac cgtgcgagtg cggatcgtaa atcacacatt gacgcagaaa
    33841 tcctttcccg gacttcaccg aaaaaatgac gcccgcagcg gtatttttca gcagacgtac
    33901 ttcaggtttt cgaaagggga aaaagcatgg ccactcctta gccaaaattt cagttccctg
    33961 aactgtgcca gaaaattacg ctggtgacta ttgctctgga aatggaaaca gtgtccttac
    34021 ccattaatcc ccgtttgtac cgaaagaata tgttgcaaac tttctaccat aatgcgcccc
    34081 gactgaaata agccttcctg gatttcattg ttaacattca cagcgtgaac cagggaggct
    34141 aataacacca cttgcaagaa ggcaaaaata aacgcaccga taatcataat atgcgtcagg
    34201 gggattttcc tttgctcctt cattttaaat aacccacaaa ctggccttct ttcattaagg
    34261 cgacttttcc tgtgtgatga taaacaatga cattatacag acccgactga taaaccgtgg
    34321 catcggcatt atcttcatct atggcacata atgaatcatt gagataagca aaattcttac
    34381 tactcattct aaaggtcatg ccaaatcctt tataaatggc tttttttgcc gcctgggcat
    34441 gttgtgcggc ttctgcattt ttaaatgcct gcgcttcaga agcacttaaa atagccgtct
    34501 ggcgctgctg ttctgcgaga taacaggtgt catggctgac gccctgcgcc tgacaatctg
    34561 cgatgcggga agcgggtgag ctacagccag ccagtaatat aaccgccagc agggaaaagg
    34621 atttaatgga gctattcatc attgttaacc ttaaataatg tgcgaattaa tgaagaggtg
    34681 aaacccggca agcttccttg ctcttattta tattgaggat tgaaatatta gaacgaatat
    34741 cctacgccga cacctgcgcc gaaatcagat tgcgtatccg cagaaatacc ggctttaagt
    34801 accgtatgcg ttccggcatt aaaagatgtt ccgacagaca ccgcagattc accgttgaag
    34861 gttcccgctc ccgctgacac catcatcacg tcaccggttt taacctgtgg ttgtgaggcg
    34921 atagcgatgg cagatgcagt tcccgcattg gcttctttac ggttgtcctc aacttcgttt
    34981 ttcagcgaac tgtaatggtt attgagcgtg ttaatttgtt ggctgttatc tgcgacccgt
    35041 ttttgcgtgt tgcttaactg ttgctgcgtt tgtgcggaat cttgtctcag actttcaatt
    35101 tgctcatgtg cctggacaga cttctgcgcc agactttgca tttgtttatc gtattgagcc
    35161 ctttcttttt gctgttgtgc tgccacatcg gttgtcagtg cattcatttt ctgcccttgt
    35221 tccgtggtag tgcgctgatt agcctcattt tgagccgcca gtctggcatt aatcatctcc
    35281 tgataatgcg cagtattttg cgtatctcca gtcgcggcga gcgttttttg tgtggctacg
    35341 atctgcgcct gttgctctgt aatttgcgtt tgctgggcat cagttaccgc agactgtcgg
    35401 gcgttgatca tgctctgata acgggcaggt gtctgtgtat cacctgtagc ggcaacggtt
    35461 ttttgtcctg cgtcaggatc tgtctggaca gtgggagtct gagtggtatc aggtgtttca
    35521 gaagggatgt agtgatgttc agtcaaatag gtatacgcag cggggctgtt gtctgtgcgt
    35581 tctaaaaagc gggtaatctt ctgctcaatc tccgattgcg acatgccaga gttaatggct
    35641 gaaagctctg ttgccgccgc ttcaatggta tcaccagcta aaacaacagg gctggatgtt
    35701 gctgaaatta tgagtgccag taaagctacg tttacagttt tcattcattt tctcttgttc
    35761 aaaata
//
LOCUS       DAZGTJ010000045        35602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000045 DAZGTJ010000000
VERSION     DAZGTJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.045"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(80..1939)
                     /gene="gss"
                     /locus_tag="ACHK8E_002741"
     CDS             complement(80..1939)
                     /gene="gss"
                     /locus_tag="ACHK8E_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP0344985.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2144..3010
                     /gene="yghU"
                     /locus_tag="ACHK8E_002742"
     CDS             2144..3010
                     /gene="yghU"
                     /locus_tag="ACHK8E_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP0344986.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3133..3381)
                     /gene="hybG"
                     /locus_tag="ACHK8E_002743"
     CDS             complement(3133..3381)
                     /gene="hybG"
                     /locus_tag="ACHK8E_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP0344987.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3394..3735)
                     /gene="hypA"
                     /locus_tag="ACHK8E_002744"
     CDS             complement(3394..3735)
                     /gene="hypA"
                     /locus_tag="ACHK8E_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0344988.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3728..4216)
                     /gene="hybE"
                     /locus_tag="ACHK8E_002745"
     CDS             complement(3728..4216)
                     /gene="hybE"
                     /locus_tag="ACHK8E_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP0344989.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4209..4703)
                     /gene="hybD"
                     /locus_tag="ACHK8E_002746"
     CDS             complement(4209..4703)
                     /gene="hybD"
                     /locus_tag="ACHK8E_002746"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP0344990.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4703..6406)
                     /gene="hybC"
                     /locus_tag="ACHK8E_002747"
     CDS             complement(4703..6406)
                     /gene="hybC"
                     /locus_tag="ACHK8E_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP0344991.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6403..7581)
                     /gene="hybB"
                     /locus_tag="ACHK8E_002748"
     CDS             complement(6403..7581)
                     /gene="hybB"
                     /locus_tag="ACHK8E_002748"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP0344992.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7571..8557)
                     /gene="hybA"
                     /locus_tag="ACHK8E_002749"
     CDS             complement(7571..8557)
                     /gene="hybA"
                     /locus_tag="ACHK8E_002749"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP0344993.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8560..9678)
                     /gene="hybO"
                     /locus_tag="ACHK8E_002750"
     CDS             complement(8560..9678)
                     /gene="hybO"
                     /locus_tag="ACHK8E_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP0344994.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9867..10154)
                     /gene="yghW"
                     /locus_tag="ACHK8E_002751"
     CDS             complement(9867..10154)
                     /gene="yghW"
                     /locus_tag="ACHK8E_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP0344995.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10273..11160)
                     /gene="yghX"
                     /locus_tag="ACHK8E_002752"
     CDS             complement(10273..11160)
                     /gene="yghX"
                     /locus_tag="ACHK8E_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP0344996.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11317..12357
                     /gene="gpr"
                     /locus_tag="ACHK8E_002753"
     CDS             11317..12357
                     /gene="gpr"
                     /locus_tag="ACHK8E_002753"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP0344997.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12397..12891)
                     /gene="yqhA"
                     /locus_tag="ACHK8E_002754"
     CDS             complement(12397..12891)
                     /gene="yqhA"
                     /locus_tag="ACHK8E_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP0344998.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13082..13966
                     /gene="yghA"
                     /locus_tag="ACHK8E_002755"
     CDS             13082..13966
                     /gene="yghA"
                     /locus_tag="ACHK8E_002755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0344999.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(14005..14430)
                     /gene="exbD"
                     /locus_tag="ACHK8E_002756"
     CDS             complement(14005..14430)
                     /gene="exbD"
                     /locus_tag="ACHK8E_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP0345000.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14437..15171)
                     /gene="exbB"
                     /locus_tag="ACHK8E_002757"
     CDS             complement(14437..15171)
                     /gene="exbB"
                     /locus_tag="ACHK8E_002757"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0345001.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15423..16610
                     /gene="metC"
                     /locus_tag="ACHK8E_002758"
     CDS             15423..16610
                     /gene="metC"
                     /locus_tag="ACHK8E_002758"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP0345002.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16750..17409
                     /gene="yghB"
                     /locus_tag="ACHK8E_002759"
     CDS             16750..17409
                     /gene="yghB"
                     /locus_tag="ACHK8E_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP0345003.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17449..18348)
                     /gene="yqhC"
                     /locus_tag="ACHK8E_002760"
     CDS             complement(17449..18348)
                     /gene="yqhC"
                     /locus_tag="ACHK8E_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP0345004.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18542..19705
                     /gene="yqhD"
                     /locus_tag="ACHK8E_002761"
     CDS             18542..19705
                     /gene="yqhD"
                     /locus_tag="ACHK8E_002761"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP0345005.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19810..20637
                     /gene="dkgA"
                     /locus_tag="ACHK8E_002762"
     CDS             19810..20637
                     /gene="dkgA"
                     /locus_tag="ACHK8E_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP0345006.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20837..21763
                     /gene="yqhG"
                     /locus_tag="ACHK8E_002763"
     CDS             20837..21763
                     /gene="yqhG"
                     /locus_tag="ACHK8E_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP0345007.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21814..22071
                     /gene="yqhH"
                     /locus_tag="ACHK8E_002764"
     CDS             21814..22071
                     /gene="yqhH"
                     /locus_tag="ACHK8E_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP0345008.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22113..24332)
                     /gene="ygiQ"
                     /locus_tag="ACHK8E_002765"
     CDS             complement(22113..24332)
                     /gene="ygiQ"
                     /locus_tag="ACHK8E_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP0345009.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24443..25855)
                     /gene="ftsP"
                     /locus_tag="ACHK8E_002766"
     CDS             complement(24443..25855)
                     /gene="ftsP"
                     /locus_tag="ACHK8E_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP0345010.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25930..26667)
                     /gene="plsC"
                     /locus_tag="ACHK8E_002767"
                     /gene_synonym="parF"
     CDS             complement(25930..26667)
                     /gene="plsC"
                     /locus_tag="ACHK8E_002767"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP0345011.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26901..29159)
                     /gene="parC"
                     /locus_tag="ACHK8E_002768"
     CDS             complement(26901..29159)
                     /gene="parC"
                     /locus_tag="ACHK8E_002768"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP0345012.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29297..30904)
                     /gene="ygiS"
                     /locus_tag="ACHK8E_002769"
     CDS             complement(29297..30904)
                     /gene="ygiS"
                     /locus_tag="ACHK8E_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0345013.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31013..31495)
                     /gene="ygiV"
                     /locus_tag="ACHK8E_002770"
     CDS             complement(31013..31495)
                     /gene="ygiV"
                     /locus_tag="ACHK8E_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP0345014.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31548..31940)
                     /gene="ygiW"
                     /locus_tag="ACHK8E_002771"
     CDS             complement(31548..31940)
                     /gene="ygiW"
                     /locus_tag="ACHK8E_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP0345015.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32092..32751
                     /gene="qseB"
                     /locus_tag="ACHK8E_002772"
     CDS             32092..32751
                     /gene="qseB"
                     /locus_tag="ACHK8E_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP0345016.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32748..34097
                     /gene="qseC"
                     /locus_tag="ACHK8E_002773"
     CDS             32748..34097
                     /gene="qseC"
                     /locus_tag="ACHK8E_002773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP0345017.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(34143..34475)
                     /gene="ygiZ"
                     /locus_tag="ACHK8E_002774"
     CDS             complement(34143..34475)
                     /gene="ygiZ"
                     /locus_tag="ACHK8E_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP0345018.1"
                     /translation="MLKKQLLSIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDDICS
                     IPSVSGEDRILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVISLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(34482..35192)
                     /locus_tag="ACHK8E_002775"
     CDS             complement(34482..35192)
                     /locus_tag="ACHK8E_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345019.1"
                     /translation="MKSDLTIKNRYCTIPQTKFRKWDEMDVLLWKLGKNDSRRRSGVY
                     YLNAYKDAYVQYNRDKIIKHAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIG
                     SLDMPENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFKIAATHL
                     RDLALFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFIYLINNLPARDTDDYKFIS
                     YGMRLLEIREHIKKLINE"
     gene            complement(35195..>35602)
                     /locus_tag="ACHK8E_002776"
     CDS             complement(35195..>35602)
                     /locus_tag="ACHK8E_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0345020.1"
                     /translation="ASIGNKYQINHVDEIFVLQFDHSLSREYNVVHHPIKFYKPIDKS
                     SPLLNAALSENEELSAVFNFYRINSGGCIERFYTIELQGAYLSNILTCYPHSITHAGN
                     QPEEVIVLNYKNITWKHHIASTESYSCWEERIF"
BASE COUNT         8812 a         9437 c         8869 g         8484 t
ORIGIN      
        1 atatataaac gttgaatgtt ttgattaatc cccgtactga ttattcttca taatcagtac
       61 ggggttgcta caacatgtat tactctttca ccacaattag cggttcaata tcactctctt
      121 ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg ccgccaacgg
      181 tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac agttgctgat
      241 agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct tcatgatggc
      301 tgacgaggtc gatgttactg ccacagcgac cggcgatcgg tttcactgca taaccggttt
      361 tcaccagttc atcattcacg gagaaatcgg tatccagcag gtaacgatgg tgcgggaaca
      421 gcgaccagag gatcggcaga atcgctttgt tgccggggat cactgtccac agaggttcaa
      481 agaccagcac ttccgggcgc agcaatacgt cgataagacg cacttcattt tgcggatgac
      541 cggtacggat cggcaccgca gcaaactcac ggtcgctaac ttcacgaatc tgatcaaacg
      601 cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc ccatcaatca
      661 gttgcccggc agcatcccag cccagttcat ccagcccgcg caagatacgc gtttcaaagc
      721 ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg atatctttgt
      781 cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca gccagttcgt
      841 taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc gcccaacgtt
      901 cgaggatcaa gccagcttcg gtatgacagg aggcggagtc ggcgttgtat tcgtagacct
      961 tcaggccacg ttcgtccata cagaaatcca tacgaccggt gatcatatgg tgacggcgac
     1021 gctgccagga gagacgcaaa cgtggccaga ggatttttgg gatgtcgaac agcgccagca
     1081 ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata catcaggtgc agttcgttgg
     1141 tggctttaat cagctcctgc tctgcgctct cggtaatggt gtagtaatga tacggatcct
     1201 gattgatcac ctgaccgttg gcctgcacat aggcgttttg tagcggatct ttttcatcca
     1261 gccatttacc gtcaaactgg cctttgtttt ccaggcgcgc tccgctgatt ttcaacagct
     1321 cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg atcatccagc
     1381 cgagaatggt ggtgtcatca aaagtgtctt taagggtata gcagccgttt tcgaccacca
     1441 tctccagctc gcgcgtccac tgttgccctt gcggtaacgg ggaatgaatc acgttctgtt
     1501 ccgcaatacg gactttgttg ccatgcaatt gggtaatgat ggcgacatga ccagtgtctt
     1561 taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca cgcggcgagc
     1621 cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttcacgc aggaagcgca
     1681 gcgagaaaat ctcccacgcc atacccacgt cagtgaagac cacaccgtaa ttcagaaaga
     1741 gaaaacggcg agcaaattca acgcattgcc atttgtggcc catatattcg tcgtcgatat
     1801 agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg taatctgaag
     1861 agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg gcatcctggc
     1921 tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag ttggcgtaat
     1981 tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga ctaatcatac
     2041 acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac agcaagtgac
     2101 ctgctacact gcttcaacac taccactcag aaggcaactc actatgacag acaacactta
     2161 tcagcccgcg aaagtctgga cgtgggacaa atccgccggc ggcgcgttcg ccaatatcaa
     2221 tcgcccggtt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac acccattgca
     2281 actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg aggagctgct
     2341 ggcgctgggc gttactggtg cagagtacga cgcctggctg attcgtattg gcgatggcga
     2401 tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg cgctgcgcga
     2461 tcatacgcat aatccgccga tccgcgtgtt tgagtctgga tcgattctgc tttatctggc
     2521 ggagaaattt ggctacttcc tgccgcagga tctggcaaag cgtactgaaa cgatgaactg
     2581 gctgttctgg ctacaggggg ctgcaccgtt cctcggcggt ggttttggtc acttttacca
     2641 ttacgcgccg gtgaaaattg agtacgccat caatcgcttt accatggaag ccaagcgcct
     2701 gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcaggcg atgagtacac
     2761 cattgcggat atggcgattt ggccgtggtt tggcaacgtg gtgttaggcg gcgtgtatga
     2821 tgccgcagag tttctggatg cgggaagtta taaacacgta caacgctggg cgaaagaagt
     2881 gggcgaacgt ccggcagtga agcgtgggcg tattgttaac cgcatcaacg gaccgctgaa
     2941 cgagcagttg catgaacgcc atgacgccag tgatttcgag acgaatacgg aagataagcg
     3001 tcaggggtaa gggttggtat tcgtcgcagc aagccatcca ggccggataa ggcgttcacg
     3061 ccgcatccgg caatcgagca caatgcctga tgcgacgctg ccgcgtctta tcaggcctac
     3121 aaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagcg cgtctaatgt
     3181 ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta ccagtaccca
     3241 ctggcccagc agatcggcag ggttaccttc acaaatcagg gcgatattca cgtcgcgctt
     3301 gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag ccagcacctg
     3361 gcctggaacg ccaatacaca ttgttaactc cggttattca acttcaatac ttttgacgat
     3421 tagcgaatcg ccggtatcga cacgcaaccg ctcgccatga cagatcggac actgcgcatc
     3481 gtgctgatga atctccacca cctggctgca atcccagcac caggcctggg cgggtttata
     3541 gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt caaaactaaa
     3601 acggacggcg ctctcctcaa cgcaggagag cgcaccaatt tccagccaca cggcggtgac
     3661 gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcgc tctggcaaag
     3721 cgacaactca tgcattttcg ccactcctgc ggccaaatag cagggcacga cgccctgcgt
     3781 gtggtacatc cggattggtg actggcagcg aaaggagcat tcgggcgcag tcatccgtca
     3841 gccgttggcc ctcttcaatc gacatgctgt gcgaaagcgg cgacatcagc gagcaggaga
     3901 ggtattgcga aacaccatcc agttcaccaa cagtaaaggt catagtgcca tacggcagtt
     3961 gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg ccggggaaga
     4021 taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct tcgaacagcg
     4081 taaagtcaga aacatacacc ggcattgaag gatgcagaaa agagagatca tgcatcgaac
     4141 gccgggcaat ttcttcaaaa gctgcctgta cttgcgcctt cggggaggtc tggaaacctg
     4201 ctatctcttc agtcatgaat cgcctcccgt gggatggctt ccacgcctga ttcacgcagc
     4261 gcagccagaa cctgctcaag cgcaggttca atcatcgctt cgaccgtcgg cgtcaagcca
     4321 atgtgcggct ccagtgattc cgggatcacg ccgaccaggg tcagcttttt cggaaactca
     4381 ccggtgaagc gcagggccga caatacgtcg gccaggccaa gctgatgcgg agagattttg
     4441 ttggtaaaca acgccggaac ttcttcatct cgcaggatca tcatcgttcc cggcgcgttc
     4501 tttttcgaga caatggcatc cgcaataatc agatgatcgc gatttgccat gtcgccaagc
     4561 agctccattc ccgccgttcc gccatcgagg atctcaacat aatccggcag aatgtatcgt
     4621 tgctctaacg cttcaacaat tcgcacaccg atggcttcat cggtcagcaa aatattgcca
     4681 acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg ttgccgtcgg
     4741 catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg gtacgcacca
     4801 cttccagcgg tttattcgga tcggcaaccg gtgtacccac cagcgactgc tcgtaaggac
     4861 cgacgtcatc attgaagtta cgcgggccag agttccaggt tgatggaaca actgcctggt
     4921 agttactgat aataccgtct ttgatcacca tccagtgaga gagcataccg cgcggtgctt
     4981 caaggaaacc aacgcctttg aactcacctg tagccggaat gttcggcttc acaaaggtgg
     5041 tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttgattttgc aggatatcct
     5101 gcaattcgca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag tgcagctgcg
     5161 ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca ttcagtttgt
     5221 tctgggtaga ctcgcgtcct gccgccagtt tcaccagcat gttagccagc ggacccactt
     5281 ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga atatttaccg tcgtcagacc
     5341 aaccatcata agccggaatg gtggtacctt cccacggtgc ctgcggcgct tcgtctttat
     5401 accaggagtg cttcgcgctc tcctgaatgc ctttaatcag gtattcatcg gagtgggaag
     5461 tgatcgggcg atacgaggac agatccgcat tctcaatgta gccgcccggg aacaggaagc
     5521 tgccgttttt gctgtcggtc gggaattccg gcacgctcag gtagttcacc gcacctttac
     5581 cgcgcgtcag ccattccggg tagaacgcgg cgataactgc ggtatcaacc ttgtaaacct
     5641 gctcaacaaa gtcgctcagt ttatcgatga aagacttgat gtacatcagg cgctcaaggt
     5701 tcagcacgcc cagaccgtcg aggttgatcg ggttagcgac cccacctact gccaggttct
     5761 gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg gttagcgtca cgctggcact
     5821 ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc agtttcatcg
     5881 ccgggtgacc ccagtagcca ttggcgaaaa tccccaactg accgctggca accagatctt
     5941 taatcttgtt ctgaactttg gtgaactctt ccggactgtt cagatgccag gtcgaaacgc
     6001 ctttcagcat ttcagaggct ttggttgggt cagcttgcag tgcagaagtg atgtccaccc
     6061 agtccagcgc cgagagctga tagaaatgaa cgatatggtc atgcgtggtg tgcgcagcca
     6121 gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagt gcactttctg
     6181 ccgcacgaac ggaagagagc gcgtgagtag tcgtacatac gccacagata cgttgcacaa
     6241 tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg cgccacatgg
     6301 taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg atgcgtaaat
     6361 gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt tgctcgcctc
     6421 atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac gaatgagtac
     6481 gatgtaagtg caaatctcaa tagccacaaa accaatagaa atcaacagtt cttcccaggt
     6541 cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc agcgaatagg tcagacgcca
     6601 ggttgcacaa cctaacagcg cgctcagtgc tgacaggaac agcatgcggg aatcattacg
     6661 cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt caatccagaa
     6721 catcgcggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat agataagctc
     6781 gccaaagcgc agcacgatga aaatcgccag caacacactg atggtgttgg tcagcttgac
     6841 gaacaggctc ttttcatctg gaccattgcc acgcagaccc gcctgcacca acgaaccttc
     6901 aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca gcggcaacat
     6961 ttcatagctc tgccacaacg gatgcacttt gtagcccgcc gagatcatca gcgaccccat
     7021 tgaagactgg tgcatagttg gcagcagcgc accgagcgcg ataatgaaga acatcacctt
     7081 gttcagacgc tgtagcgaca ccttccagcc caatcgttca aacaatgccg gagcaaactc
     7141 cagtgccatc acgccgatgt agatggtcat acagaccgcc gtctcgaaca gtaccgagtt
     7201 cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac caacgtcgat
     7261 ggtgatcgac aagccaccca gtgagtaacc gaacagactc gccaacagcg ccggacgcac
     7321 cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg cccagccgcc
     7381 acaggcaaag ccggtgccga tcaacaggtc aaacgcgatc cacacgcccc acgggaagcc
     7441 gccgttcagg tcagagacag agcccagacc gaacaccaga cgcttcacaa tcaggagcat
     7501 acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt tgccgcccag
     7561 cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt ttggtgttgc
     7621 gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg cctttataca
     7681 gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt ttcggcaggt
     7741 cgagattttc gtaaggcaca ccggtcagta ccagaacctg agtaccgccg ccctctttct
     7801 cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg ccagatttca
     7861 gcgtctgacg cggatagtgg tattcgctgc ctggcttcag cgccagacgt tttttcgcct
     7921 cagccatcag ctcttcacgc gtaccaaaaa tcaccgcgcc cgccgggcac acttctacgc
     7981 agccaggcag accgccttta tcaagacgtt ccacaccttt ctggttgcac agttcacact
     8041 tatgcagcgc accaaatggg ttgttgtagt cgtacttcgg cacgttgtat gggcaggcga
     8101 ccatgcagta acggcagcca gtgcacacat ctttgtcgta atggacaatg ccggttttcg
     8161 gatctttttt cagcgcagag accgggcaca cagagacaca attcggatcg acgcagtgca
     8221 tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg acccccgagc
     8281 cgctggtcca cacctgaatg atgttattgg tgtacggcga cagtttgtcg ttgttcgacc
     8341 aggtctgttc cccttgcggg ttacgttcag ggaaattgat atcctgacac ttggtgacgc
     8401 aagcctggca gccaacgcac aaggtcgagt cgtacaacat ccccagcgat cccggaattg
     8461 gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc agcaatgccc
     8521 cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc gtgagtcagc
     8581 gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga caccggcaac
     8641 cagcccaacc acaccgccga gcaaaccaat agcgcccgca gagacgttgc cgccttcttt
     8701 agcgttaacg tccggtttct gtgaacgcgg agtctgattt tcgacgttgg caagctgatg
     8761 gatgccttta tggaagccga taccttcttc gttacagcca tagcacgggt gaccaatcgc
     8821 taccggccac acgccgccaa cgtcgcagaa ttgcagcgtt gagcagttgc cgtatgtttc
     8881 tggcccttta cagccgaggt ggtacaggca ccagccttcg cggtggcctt catcaccgaa
     8941 ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt cgtgaatcag
     9001 acggccatag gcgaaggtcg gacggttttt gtcatccagt ttcggcggct tgccgtaagt
     9061 gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc ccggaatatt
     9121 gataacggtt ttgcctggca gaacttcttg caggctgact gcgccagttg ggttaactcc
     9181 agcggcggca acaccgcccc acgcagagca ggaaccgata gcgataatgg ctgctgcgcc
     9241 ttccgccgct ttgcggatgt gatccacaat cggctcaccg gcaaccatgc aataaatacc
     9301 gttatctttt aatgggatgg aaccatccac cactaacaca tactgccctt tgtacttctc
     9361 gagagcgtta tgtttgttct cttcgacctg gtgaccgaaa gcagcagaaa gcacctcgtg
     9421 atactccaga gagatagtct ccagcacgag gttttctacc gttggatgcg ttgcacgaag
     9481 cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg gcggacgctg
     9541 cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc ccatggtggc
     9601 ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag aatggatgag
     9661 ggtgttatct ccagtcattt atagttattc cgttgcgaag acctggcata tattttgcct
     9721 caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat tatggttatc
     9781 gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag tgtaattaat
     9841 taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc cataaaaaag
     9901 cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc tttatccagt
     9961 ccatagcggc gcgtcagaat acccgcttcc caggcgctaa gctggcgatc tccggtcttt
    10021 tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt tataatcggc acagtatttt
    10081 gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa gcctttacca
    10141 aaatggttat tcatcgttca cctcattcgc tatatattgc cgtatatagc gatattttag
    10201 gcaaagaacg acatttgcgc acgctatttt ttcatgcttg tttaagacgt aatcctgtaa
    10261 gagataaacc catcaggaga gatatttatc gaaccatttc agcgtccttt gccaggcaag
    10321 atcggcggca gatttgtcat aacggggcgt ggaatcatta tggaatccgt gattaacccc
    10381 cggatatata tacgcctcat agaccttatt attggctttc aacgccgcct cgtaagcagg
    10441 ccagccctcg ttgattcggg tgtccagttc cgcgtagtgc aacagtaaag gcgcttcaat
    10501 cttcgccaca tcggcagtgg gtgcctgacg accataaaac ggcaccgcgc aggccagttc
    10561 cgggtaagcg accgctgccg cgttcgatac gccaccgcca tagcaaaatc cagtaatacc
    10621 cactttgcct gtcgcttgcg gatagctttg cataaactca atcgcggcaa agaaatcatt
    10681 catcagtttg gttggatcaa cctgctgttg cagctcacga cctttatcat cattacccgg
    10741 ataacctcca acggaactta agccgtcagg tgccagggcg atataccccg ccttcgccac
    10801 tcgccgcgtc acatcttcga tatacggatt cagccctcga ttctcatgca ccactaccac
    10861 ggctggcgtt ttgccgctca tttttgcggg cttcaccaga taaccccgta cctcgccgtg
    10921 accatttggc gagggatacg tgatgtactc tgcaacaatt tccgggtcgg taaactctac
    10981 ctgagtcgcc agcgcataat ttggcttgag caaatcaaac agcgctaatg ccgtcatccc
    11041 gcccaccgca tacttcgccg caaggttgag gaactcacgt ttcgagattt tcccgtgagc
    11101 gtaatagtcg tagtaatcca gcaactcttg tgggaaatct ttggcggtta aacgcggcat
    11161 cgttgcactc ctcaactggt gtttttttaa gcaaagcata agcgcgtgtt tttgcccagt
    11221 ttttcgtcac tctgtgagcc agactacggg atacgcgctg gcgaatcgct aaactagaaa
    11281 cattgtttcg aaattgaacg atggaaagga gaggtcatgg tctggttagc gaatcccgaa
    11341 cgttacgggc agatgcagta ccgctattgc ggaaaaagtg gtttacgcct gcccgcgtta
    11401 tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc tggaatcaca gcgtgcgatc
    11461 ctgcgtaaag cgtttgattt aggcattacg cactttgatt tagccaacaa ttacgggccg
    11521 cctccaggaa gcgcagaaga gaactttggt cgcctgctgc gcgaggattt tgccgcttat
    11581 cgcgatgaac tgattatctc taccaaagcc ggatacgata tgtggcccgg cccgtacggc
    11641 tctggcggct cacgtaaata cctgctcgcc agcctcgacc aaagcctgaa gcgtatgggg
    11701 cttgagtatg tcgatatctt ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa
    11761 accgcctctg cgctggctca tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc
    11821 tcttactcgc cagagcggac gcaaaaaatg gttgagttgc tgcacgagtg gaaaattccg
    11881 ctgttaattc atcaaccttc gtacaattta ctgaaccgct gggtggataa aagcggcctg
    11941 ctggataccc tgcaaaataa cggcgtgggc tgcattgcct ttactccact ggctcaggga
    12001 ttgctgaccg gaaaatatct caacgctatt ccgcaagatt cacggatgca tcgtgaaggg
    12061 aataaagttc gtggtctgac gccgaaaatg ctcaccgaag ccaacctcaa cagcctgcgc
    12121 ttattgcatg aaatggcaca gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg
    12181 ttgctgaaag atgagcgagt gacgtcggta ttgattggag ccagccgcgc ggagcaactt
    12241 gaggagaacg tgcaggcgct gaataatctg acatttagca ccgaggagct ggcgcagatt
    12301 gatcagcata tcgccgatgg agagctgaat ctgtggcagg cgtcttccga taaatgacct
    12361 gttaataacg ggcgcgagaa ccgcgcccga atcagatcag tgattatgac gagtcagtcg
    12421 gtcaagatag cccatcacaa atgcagagag cacaaacgtc agatggataa tgacgtacca
    12481 catcagtttg ttatcaggga catttttcgc atccataaag acgcgcagta agtgaatgga
    12541 agaaattgcc acaatcgacg ctgctacttt gtttttcagc gacgttgcgt ccattttccc
    12601 cagccagttc agcttctctt tgttctcaga gatatcgagt tgtgagacga aattctcgta
    12661 accggaaaac atcaccatca ccagcaaacc gccaaccagc gtcatatcca ccagcgacag
    12721 caacacgagg atcaaatctg attccgccat cgagaagata ttcggcagta cgtgaataat
    12781 ctcctggaag aacttcagcg ccagggcaac taacgccagc gaaaggccaa agtacacggg
    12841 ggcaagcagc cagcgagaag catacattgc attttcaaga aaacgttcca tacattcctg
    12901 tcgtgtagtt aaaacgggca acagtatatc gcaattaagt aaacagcacg caacagcaaa
    12961 tcaacatata cgttaaacct ttgtcgcata attctgaggg ttagggatct tcctggttcc
    13021 cttatcaacg catcgagagc gtctaccctt aaaggacgtt gaacctgaag ggagaaaaat
    13081 gatgtctcat ttaaaagacc cgaccacgca gtattacact ggtgaatatc ccaaacagaa
    13141 acaaccgacg ccaggcatcc aggcgaagat gacaccggta ccggattgcg gcgagaaaac
    13201 ctatgttggt agcggtcgcc tgaaagatcg taaagcactg gtgacagggg gcgattccgg
    13261 aatcggtcgc gctgccgcca ttgcttacgc gcgtgaaggg gctgacgtgg cgatcagtta
    13321 tcttcccgtg gaagaagaag acgctcagga tgtcaaaaag atcattgaag aatgcggacg
    13381 caaagccgtt ctgctgccag gcgatttaag cgatgagaaa ttcgcccgtt cgctggttca
    13441 cgaagcacac aaggcgttag gcgggctgga tattatggcg ctggtcgccg ggaaacaggt
    13501 tgccattact gatattgcag acctcaccag cgaacagttt caaaagacct ttgccattaa
    13561 cgttttcgcg ctgttctggc taacccagga agcgatcccc ctgctaccga aaggtgccag
    13621 tatcatcacc acttcgtcaa tccaggcata ccagccaagc ccgcatttac tggactatgc
    13681 ggctacgaag gcggcaattc tgaactacag ccgtggcctg gcaaaacagg tcgcggagaa
    13741 aggtattcgg gtgaatattg tcgcgccagg cccgatctgg acagcactgc aaatttccgg
    13801 cggacaaacg caggataaga tcccgcagtt tggtcagaaa acgccgatga agcgtgcggg
    13861 gcaaccggcg gaactggccc ctgtatatgt ttatctggca agtcaggagg cgagctacgt
    13921 caccgcagaa gtgcatggcg tgtgcggcgg cgagcattta ggctaaaaaa atgcccggtt
    13981 gtgaaaagca accgggcatc atttttattt cgctttggca gtttcttcgc cgaccagacc
    14041 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    14101 gacggtttta tccgctcgga agaagacggt ggtgtctttc ttgccttcgg ttaacgcatt
    14161 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    14221 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    14281 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    14341 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    14401 gtcgaggttt tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc
    14461 gacacgaacc ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa
    14521 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    14581 tacgttatag ataacgaccg ccggaatcgc tgccacgagg ccgattgccg ttgctaacag
    14641 agcttctgcg atacccggcg caacgactgc caggttagta gtctgcgttt gcgcgatacc
    14701 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gcgaaatcgc
    14761 gccgatggtt gccagatagc cgttaccacg gcccatttga cgacctactg cggcgacccg
    14821 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    14881 cagttccagc tcattctgcg cttcattgag caaatgcagg ctcaagcttt tgctaccaaa
    14941 atcagcggcg atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc
    15001 gcgcttaaga cgacgcttct gattgaagaa ctccacgctc ttactgaaga agattgccca
    15061 ggtgactacg gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg
    15121 ctgatacata ccccagacgg agaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    15181 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt
    15241 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    15301 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    15361 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg
    15421 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    15481 cgctcggtgc ggtaaatagc gtgattcagc gcgcgtcttc gctggtcttt gacagtgtgg
    15541 aagccaaaaa acacgcgaca cgtaatcgcg ccaatggcga gttgttctat ggacggcgtg
    15601 gaacgttaac ccatttctcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    15661 gtgtgctatt tccctgcggg gcggcagcgg ttgctaattc cattcttgct tttgtcgaac
    15721 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    15781 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    15841 tcgttaagca tctgctgcca aacactaaaa tcgtgtttct ggaatcgcca ggttccatca
    15901 ccatggaagt ccacgatgtt ccggcgattg ttgccgccgt acgcagtgtg gtcccggatg
    15961 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    16021 gcatcgatgt ttctattcag gccgccacca agtatctggt tgggcattca gatgcgatga
    16081 ttggcactgc ggtgtgcaat gcccgttgct gggagcagct gcgagagaat gcctatctga
    16141 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    16201 gtgtgcgttt gcgccaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac
    16261 atccccaagt agcgcgagtt aaccaccctg ccctgcctgg cagtaaaggc cacgaattct
    16321 ggaaacgaga ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    16381 atgatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    16441 ggggcgggta tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gctattcgcc
    16501 cacaaggcga gatcgatttt agcggaacct tgattcgcct gcatattggt ctggaagatg
    16561 tcgacgatct gattgccgat ctggacgccg gttttgcgcg aattgtataa cattgccact
    16621 tttggacaat tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca
    16681 aggcgtcccg gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    16741 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    16801 gcgctggcgg accctcatat tgttagcgtt gtttactttg ttatgtttgc cacgctgttt
    16861 ttagaaaacg gcttgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    16921 ggcgcattga ttgcccaggg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    16981 gcagcaagtc tgggctgctg gctaagttat attcaggggc gctggttagg gaataccaaa
    17041 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    17101 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    17161 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    17221 tggttaagcg gattgctgtg ggtcagcgtg gtaaccagtt tcggctatgc cttaagtatg
    17281 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    17341 gccttgttaa ccgctggctt gttgggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    17401 aacgcctgac gattttcccc attcccggta gctgtaccgg gaacgtattt aattcccctg
    17461 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    17521 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gccgccgcgc tggccttcat
    17581 gccgtcgtgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    17641 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    17701 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag
    17761 cacgcgacta atcaggctga agtgagtctg gcggctgacc agtgccagta acgcgccgcc
    17821 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    17881 cgcatccagc ggtcgctcca tcacatccag taaccgctcc gccgcgcata aaatctcttc
    17941 tgataacgtg gcagagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    18001 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    18061 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    18121 gagatattca ttagcgtcat aacgaaacac gcgttcattg atataaccga ttttatgtcc
    18181 ggaaaagaga attatgatgc caggctcgta catcaccggt gtgcgtgcga aaggcgtctc
    18241 gccatacaac aaacgcacat cgggcaacag ctctgacaaa ctattttctt tatttttcag
    18301 tttattaact ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt
    18361 cttaggaata atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg
    18421 gagaaatagg caagacattg gcagaaatga gcattgagag gtacggcgct ggcgaccaca
    18481 atgaaaaaca tcaggcagac cgttctctgc cctcatattg acccagcaaa gggagcaagt
    18541 aatgaacaac tttaatctgc acaccccaac ccgcattctt tttggtaaag gcgcaatcgc
    18601 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gcggcggcag
    18661 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    18721 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    18781 ggttcgcgaa caaaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    18841 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    18901 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    18961 agcaaccggt tcagaatcca acgccggcgc ggtgatctcc cgtaaaacca caggcgacaa
    19021 gcaggcgttc cattctgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    19081 ctacaccctg ccgccgcgtc aggtggctaa cggtgtagtg gacgcttttg tacacaccgt
    19141 ggaacagtat gttactaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    19201 tttgttgacg ctgatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    19261 gcgcgccaac gttatgtggg cggcgactca ggcgctgaac ggtttgatcg gcgctggcgt
    19321 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    19381 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgataccaa
    19441 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttcagacga
    19501 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    19561 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    19621 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgctgg atgtcagccg
    19681 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    19741 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    19801 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    19861 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    19921 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    19981 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    20041 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    20101 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    20161 gaagcatgga agggcatgat cgaattgcaa aaagagggat taatcaaaag cattggcgtg
    20221 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtt
    20281 attaatcaga tcgaacttca cccgctgatg caacaacgcc agctacatgc ctggaacgcg
    20341 acacacaaaa tccagaccga atcctggagt ccattagcgc aaggtgggaa aggtgttttc
    20401 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    20461 atccgctggc atctggatag cggcctggtg gtgatcccaa aatcggtcac accttcacgc
    20521 attgtcgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact tggcgaaatt
    20581 gcaaaactcg atcagggcaa gcgtctcggc cccgatcctg accagttcgg cggctaacgt
    20641 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    20701 cctcgcgtcc tgcccattgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    20761 tcatgcagag ccggaagctc ttaaaggaac aactcatcta cacccgggat aaacgcaacg
    20821 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    20881 cgtacacgcg cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa
    20941 ctataaatca gatgccagta cgccttattt tggtgaaacc ggagagcgag cgataacttc
    21001 tgcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acgctgccgg gcaatattgg
    21061 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag
    21121 cgtagcgata acccaacctg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21181 taaagacgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21241 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21301 tgaaaaaacg ctggccgcta tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21361 cgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    21421 ttaccgccag gcggtcaatg ccttctataa aaccaccttc aagactacca acaatcccga
    21481 tgaagatatg cgaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21541 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21601 cattcatgtt cgttttacgc tgaccaatgg caacaatgaa gagcaagaac tggttttaca
    21661 gcggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaatagcg gtagcctact
    21721 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgagt taaataacaa
    21781 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    21841 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcagctg
    21901 gcgagcaatg tgcaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    21961 ttacgcccac aaatctatgc tgccaaatcc gacgctaaca gagccaatac gcgtcttgat
    22021 gctcaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    22081 tcccggcagc attctgttac cggggatatt ttttaacgtc caaccgccgc tttagggcgt
    22141 ttcttcgcac cagcattctg caggcgagat tgcgtagacg acgctttttt tgccgtagca
    22201 ggcgtctgac gctgggtcgc catcggcgta tgtttagtca acgccggacg ggtattgcgg
    22261 ttctggcgac gagcctcacg catctcttca atggttggcg caggcactaa gcaatcgcga
    22321 cggctgccaa tcagatgctt tttgcccatc gcttccagcg cctggcggat taacggccag
    22381 tttgccggat cgtggtaacg caacaacgct ttatgcaggc gacgctgctt gtcgcccttc
    22441 ggtacaaaga cgtcttcact tttataacca atcttcgcca gcgggttttt gccggtgtaa
    22501 tacatagtgg tcgagttagc cagcggcgac gggtagaagt tctgtacctg gtcgaggcgg
    22561 aagcggtgct ttttcagcca cagcgccaga ttcaccatat cttcatcgcg cgtacccggg
    22621 tgcgcggaga tgaaatacgg gatcagatac tgttctttac ctgcctgttt cgagtaagta
    22681 tcgaacagct ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgataac
    22741 ggcccttctt cggtatgttc cggggcaatc ttcagataac cgccaacgtg atgggtcgcc
    22801 agctctttga tatagcgcgg atcttctacg gcgatgtcat aacgcacacc agaggcaatc
    22861 aggatctttt taatgccttt cagatcgcgc gcacggcgat agaggttaat cgtcggttcg
    22921 tggttagtgt ccatatgcgg acaaatatcc ggataaacgc acgacaaacg acggcaggtt
    22981 tgctcagcgc gtggcgattt gcaacgcaac atatacatgt tggcagtagg cccaccgaga
    23041 tcggaaatca cacccgtaaa acctggaaca gtgtcgcgga tcgcttcgat ctcattaata
    23101 atcgaatctt cggaacggct ctgaataatg cgcccttcgt gctcggtaat agaacagaaa
    23161 gagcagccgc caaagcagcc acgcataatg ttgaccgaga aacgaatcat ttcgtaagcc
    23221 ggaattcggg cattaccgta agccggatgc ggcacgcgct tgtacggcag cgcaaagacg
    23281 ctatccatct cttcggtaga aagcggaata gcaggcgggt tgatccatac atagcggtcg
    23341 ccatgttttt gcatcaatgc gcgggcgcag cctgggttag tttcgtggtg aagaatgcgc
    23401 gaagcatggg cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaacacg
    23461 taggtttttt cccacggttt cgggcgcggc ggctgcacgg ttacggcttt cgcttcctgc
    23521 tttttcggcg caacaggttt gttatccgcg cacggcagat cttcgccata cggatgcggg
    23581 atcgggtcga ttttcccagg agtatcaaga cgggtggaat ccacgccgct ccagcctggc
    23641 aacgcctctt ttacgataat cgcggtatta cgcacatcgc ggatttcact aatcggctcg
    23701 cccatcgcca gacgatgcgc cacttccacc agcggacgct caccgttacc gaacatcagc
    23761 atgtcggctt tcgaatccac cagcacggaa cggcgcacgg tatcggacca gtaatcataa
    23821 tgcgcggtac ggcgcagact tgcctcaata ccgccgagga tcaccggtac atctttccac
    23881 gcctctttac aacgctgggt ataaaccagt gtggcgcgat ccggtcgctt acccgcgacg
    23941 ttatccggcg tgtaggcatc gtcatgacgt aaacggcgat cggcggtata gcggttgatc
    24001 atcgagtcca tgttgccagc agtaacaccg aaaaacagat tcggtttacc cagacgcata
    24061 aagtcgtctt tgctgctcca gtccggttgg gcaatgatcc cgacacgaaa gccctgcgct
    24121 tccagcatac gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca
    24181 gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgagacatc
    24241 ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg
    24301 cgatccggtt ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa
    24361 tcaaaggccg gggattataa gccggaacga aagagaaatc gaaaggtatt ccatactcgc
    24421 cctcctcggg cgagtatgaa gattacggta ccggattgac caacagttgc cctatcgagc
    24481 cacggtccgc catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag
    24541 gctgaccgaa ataaaccagc agctccactt gtccgtcaac ccaaacggta tctttccagc
    24601 ctctgtcttc cgggaacggc atcgcgccat tcacgttacg gatctggaac atcacgcctt
    24661 caatatggaa cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct
    24721 gcgcggtgac atcaatacgg ttgacgtccc acagttgtcc attaataccc gggtcatcac
    24781 ccagactgat atcgcgactg cgaatcggcg aaccggccat gatttcagtt ggcagcaggc
    24841 gcatcggaag actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca
    24901 gggtagaaac cagaatgctg gatggctcaa agaagccgcg aatacgatca acaatgctcg
    24961 ccgcttcacc acaggtgatc gacacttcat caccgttgct catatccacc agaatctcgc
    25021 ggcgctcgcc cggtgccagc gaaagttgct tcaccgatac aggagcgggc aggaatccct
    25081 gatcgccaga aatcacatgt aacgggcgac catcgctcat ctgtagttga tagcgacgag
    25141 agttcgacgc gttcaacaga cgcaagcgca cccagccacg cgagacttca acgtacgggc
    25201 tttgtacacc gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttat
    25261 attccggcgt accaaagtta tccagccgtt tatcctgaat aatgaccgga aaatcatcca
    25321 caccataatg gttggggata ggcagcgatt tgctgacttc atcttccacc agccacattc
    25381 cggcaaggcc gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca
    25441 gagtagctgc gttctggcga atgggcagta cgggtgccca gtcagcgttt ggcgacatca
    25501 tccgtgccgg accgcccatc agcgggccag gtacctgtag cccggccacc gtcattgaga
    25561 cattttctgt caggcgattg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga
    25621 tagtcggccc caggtaacga ccattgattc cccaaaccga tgcgcgtgtc cctggcgtaa
    25681 atgaccagtg cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagca
    25741 gcgtcggaac gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc
    25801 ctgcacaaag tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg
    25861 ctccatgtaa aactggctaa agtaataccg tgcaggaaag tgttccccgc aaaaatacag
    25921 attgccgact taaacttttc cggcggcttc gcgctctgcg acttctttat cgagctcagc
    25981 gattttttgt tccattatcg aacgacaatg ggcagccaac tcgcgaacct gatctttgcc
    26041 atactgactg acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgtagccg
    26101 attaagatta atcttattcg aagttgtaga gacgcacacg ggaataatcg ggacgcccgc
    26161 cgcaattgcc gcgtgaaatg ccccggtctt gaacggcagc aggccgcgac cacggctgcg
    26221 ggttccttcc gggaacatcc agatggaaat acggcgtttt ttgaagtgat tcacgacttc
    26281 cgcaatagtg ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa
    26341 ccagtacaac tgcccgaaga aggggatcca cagcaagctc tttttcccta ccgtcactgt
    26401 cggcggctgc acaatgttcg atgccgtcac catgtcataa ttgttctggt ggttagcaat
    26461 atagatagca tttccgtagc tttcagcatc cgcaggtttg cggcactcaa ctttcaggcc
    26521 gaacagcggt gcaagacggc caaacatatg cccaaaggtg gccacatgtt tcgggttacg
    26581 cgggctgaaa aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt
    26641 aataataaga cgaaagatat atagcataac accctctgaa ggtctgagag actggcattg
    26701 tacgtcacct gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg
    26761 ttaatcgcaa cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt
    26821 gaatacgctg ccggatgacg acttaacgtt tcatccggcg ttccttgcaa gcgggaggaa
    26881 acagcgccct ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag
    26941 tcgatctcaa cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt
    27001 tcgccagcga ctttctgtag ctcttccgga cgcagtttaa ttttgcgttt cccaacatga
    27061 atggtcagcg tgctttgcgg cggcagaacg tacagttgcg caagaccatc ctcgccacgc
    27121 gcggcttctg ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc
    27181 agatcgctta ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg
    27241 gaagcatctt caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa
    27301 gccttacctg cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc
    27361 gcatcggaag ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc
    27421 gctccaggcg gcaacgttaa tttgccggtg agcggctcac cctgaccacg cgccgacggc
    27481 agcgtaatcg ggtcgatggc atagctacga ccggtggaat caacaaacac taccggttgg
    27541 ttgctcttac ctttcaccgc cgctttgaag ctatcgcccg ctttataatt caggcccggc
    27601 gcgtcaatat catggccttt agcgctgcgc acccagccca tctgcgacag cacaatggtg
    27661 acaggttcag acggcagcat gtcgtgctcg ctcattgctt tcgcttcttc gcgttcctgc
    27721 aacggcgaac gacgttcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc
    27781 aggttattca ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc
    27841 agctcactct gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc
    27901 agttcgagga tcgcttccgc ctgggtttca gtaaggccaa accgcgacat cagcgccggt
    27961 ttcggttcat cttcattacg aatgatctca atcacttcgt cgatattgag aaacgccacc
    28021 agcaaacctt cgaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg
    28081 cggcgcacgg tatcgcggcg gaacaccagc cattcggaga ggatttccag caggtttttc
    28141 accgccggac gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga
    28201 tcggtggtag caaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc
    28261 acaatcacca gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc
    28321 ggcagctttt tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc
    28381 tgatgcggca atgcgctgat aaccaccgcg ccatcttctt tcttccaaac cgcgcgcata
    28441 cgcactgaac cacgtccgtt ctcgtagatt ttacgaatct cggcgcgcga agtgataatt
    28501 tccgcttcag tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt
    28561 ttcggctggt cgattaatgc gattgccgcc tgagccactt cacgcaggtt atgcggtgga
    28621 atatcggtcg ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga
    28681 cgggcaggca gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc
    28741 gtcccctgcc ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg
    28801 taacgcattg ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca
    28861 accagcggat aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggca
    28921 ctatcgccgt gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta
    28981 aatttggcgc tggcattcag gcccagctca gacatcgcat acacaatgcg gcgctgaaca
    29041 ggtttcagac catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag
    29101 tttaagtagg cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt
    29161 aattctgatt cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta
    29221 cattatctgc cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc
    29281 ggcggcgata aaggcatcaa tgtgccttga tatacaactc tttgctgtag acataatcct
    29341 ggggattatg cagcggaaaa ccgccaacgt agggtttaag cagtttgatt aacggctgat
    29401 agtagatggg aatcagcggt gcctgctggt tgatgatcac ttctgcctgc tgatacaacg
    29461 cattacgctt tgtcgcatca gtgatctgcg tggcctggtt tagtaaggcg tcatactgcg
    29521 catttttcca gtgaccgacg ttttcttcac tatcgctttt gagcgtgttc aggaagctgg
    29581 aggcatcatt gtacgtcgca tcccacgact gccgagacag cataaaatca ccggctcgtc
    29641 gggcatcaag ataggttttc cactccattg tgcgcagcgt cacctgtgca cccagccatt
    29701 ttttccattc ggaagacaac gctatcgcgg tcttttcatg cagatcgtac ttgttgtaga
    29761 acagctcaaa gcgtagcgga tgagaggcgt cgtatcccgc ctgtttcagc aaggcttttg
    29821 ccatcgcgac acgctcactc attggctttt gcagttcatc gaacgtcgtc gcgctaaagc
    29881 cttttacctc tggcggcgtc agcgtggttg cgggcgttct caaccccagt accttttgcg
    29941 caataagctg tcgatcaacc gtaagatata gcgcccgacg cacccgcaca tcgttaaatg
    30001 gcggtttctc aaggttaaag ttgtaatatt cgctgttcag acgcgggata attcgtagct
    30061 cgccaggcag tgatttttca atggcgggaa tttgctgcgc cggaacccag gtgagatcga
    30121 cctctcccgc gcgatagcgg ttatagccgg tgaccgaatt atccagcgcc agatactcaa
    30181 cctgttgcaa taccgtatgt tgcgcatcgc ggtactttgg atttttgcgt gcggtaatct
    30241 tttcgttaac tacccattta tcaagcacaa aggcaccgtt gtaaaccatg ttctctggct
    30301 tactccagct atcgccatgt ttagcgatga catgatgagg aaccgggaac aacgtcggcc
    30361 aggcgagcat cgtcgtaaac caaggaactg gctgctcaag cgtaacttca agagtacgat
    30421 catccgtcgc tttgatcccc agcgatgtaa catctgcttt acccgcaaca atagccgcgg
    30481 cattgttaat gtgcgcctgt gccagatagc cagcaaaagg gcttgccgtt ttcgggtcaa
    30541 ccgcacgttg ccagccgagg acaaaatcct ctgccgtcag aggctgaccg tctgaccact
    30601 gcaaaccgct acgcagatgg aaaatatagc gcttgccgcc gtcaagtatc tcccagcgtt
    30661 cagcctgagc gggctgcacc tggccttcac cgtccatcca taccagacct tcaaacagat
    30721 ccagcacaat ctgcgcggca gtattctcct ctaccttttg cgggtcgagc gtgcctgggt
    30781 cgctatgatt gttgtaacga aagacttgtt gcggggcgag cggtgtgttg gcgggaacgt
    30841 cagcagcgta gagaggagcc gcacttacca ggctgaccag ccataataaa tttcgcgtat
    30901 acatggtatt ccttaccgga gaaattataa gcccgacaac aatacgcctg tggcattgtt
    30961 tgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcca
    31021 acggcacata aatatccgtt tccagtcgcc gctctgtcat cccttcggca agattggtgt
    31081 agtgaaacag gatcggggct ttgcgcattt tttcgccgct tgcaggtagc cagtggcgaa
    31141 tgatgcccca tatcgtgtgt gaaatatcgt ccagctcgcc aacatggcgg gcaacagcat
    31201 aacgcccacc ggtaagttcg ccattactca cgccataacg attatcgggg atcggttcgc
    31261 taacgctgcc gcagatatca aagcgaaacg cttctggtgc ggtggtggct gggtcgtccc
    31321 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
    31381 tgataaattt cgccacgctg tagttgagca actcagggct acagcggtgc ggcaacatcg
    31441 ccacagggat tgatgggaaa tcgataatat ttacatccag tgtcaggttt gtcattttgc
    31501 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
    31561 ttgcggatct gtttgacgtc aatttcaaca gagttccagt ctttatcgac ttccccctga
    31621 atctcaaccg tatctttcgg cgtcaccgtc acgccgttcc agcgtttatg gtcgatatca
    31681 acattgatag taccgctggc atctttgaac acgtagagat cgtcagagat gcgttcaacg
    31741 atattgccgc gtagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
    31801 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
    31861 gaaaaacctc cctgctctgc tgccataacc ggtgcactgc acagggccat tactgcgatt
    31921 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
    31981 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
    32041 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
    32101 ttactgatag aagatgacat gctgattggc gacggcatca aaacgggcct tagtaaaatg
    32161 ggttttagcg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct atatagcgca
    32221 ccttatgatg cggtgatcct ggatttaacc ttaccgggca tggatggtcg cgatattttg
    32281 cgcgaatggc gagaaaaagg tcagcgtgag ccggtattga tcctgactgc gcgcgatgcg
    32341 ctggcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
    32401 gcgttgatag aagtcgccgc caggctggaa gctctgatgc gccgaaccaa cggccaggcc
    32461 agcaacgagc tgcgccacgg caacgtcatg ctagaccccg gcaaacgtat cgccacgctg
    32521 gctggcgacc ccttaacgct gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
    32581 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
    32641 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
    32701 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
    32761 aacgtcttag tctgcgagtc aggctgacgc taatcttttt aattctggcc tcggtgacct
    32821 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gagttgttcg
    32881 acacccaact gatgctgttt gccaaacggt taagtacgct cgatctcaac gaaatcaacg
    32941 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
    33001 cgctgacttt tgccatcttt acccacgacg gcagaatggt ccttaacgat ggcgataacg
    33061 gagaagatat tccttatagc tatcagcggg aaggttttgc tgacgggcaa ctggtcggtg
    33121 aagacgatcc ctggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
    33181 tgggtcagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc ggacaattga
    33241 tcccgtggct ggtcgcactg ccaattatgt taatcatcat gatggtacta ctgggtcgtg
    33301 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    33361 aaccactaaa cgcgacgggc gtacccagcg aagtgcgccc actggttgag tcgctaaatc
    33421 aactgttcgc ccgcacacat acgatgatgg ttcgtgaacg acgctttacc tccgacgcag
    33481 ctcacgaact tcgtagcccg ttaacggcgc tgaaagtgca aaccgaagtt gctcagctct
    33541 ctgacgatga tccgcaggcg cggaaaaaag cgctgcttca attacattcc ggaatcgatc
    33601 gtgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    33661 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    33721 accacacggc gcagcaggcg aacattgacg tgcgactgac actcaatgcc aacggcatca
    33781 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    33841 tgcgctacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    33901 tgagggataa cggccccggt gtgacaccag aggcactggc gagaattggc gaacgcttct
    33961 atcgcccacc cggacaaacc gctaccggca gtggacttgg gctatcaatt gtccagcgaa
    34021 tcgccaagct gcatgacatg aatgttgaat ttgggaatgc ggaacaaggt ggatttgagg
    34081 cgaaagtaag ctggtaactt ctctgtctat gagagccgtt aaaacgactc tcatagattt
    34141 tattaatagc aaaatataaa ccgtccccaa aaaagccacc aaccacaaac caaacaaata
    34201 acgagcgaga ttacatataa ccaaaactca aacatttctc gcacaaagag ttttcttaag
    34261 ataataataa taagtggcgt cagaagaaaa aatgctgcaa tcattgcttg caatatacga
    34321 tcttcgcctg aaacggaagg tatactgcaa atatcatctc ccgactcaag catccagtca
    34381 tacttattga aagcaaaaaa agcatctatt aaccaataag taagcattat gtataatagt
    34441 gcttcaaata ttgatagtag ttgttttttt aacatctctg attactcatt aatcaatttt
    34501 ttgatatgtt ctctaatctc taacaacctc ataccataag agatgaattt ataatcatct
    34561 gtatcacgcg caggcaaatt attaattaaa taaataaaaa aacccaaatc tcgttcaaca
    34621 ccacgattat atctaatacc agccataata tactgctcat tcgtcatata cagtgttgcc
    34681 gaagagggat aatcaaaaag ggcaagatct cttaaatgag tagcagcaat tttaaaggta
    34741 aaatcatctt ccatcaggca tgtcgctaac tctaactgat ttctggtagt taattctgaa
    34801 gggtcaaggc ctagagtaat cgctgcggtt cgtatttgta tacccattga gccaaatgat
    34861 gttttatttt caggcatatc taacgatccg atcattcttt ttgtttcata tatctgaaac
    34921 ttataatttt caggcatacc tcctgactca atccaggcca cgccgcccag taactctgga
    34981 cgaatccctg cggcatatgc atgtttaatt attttgtccc tattatattg tacatatgca
    35041 tctttataag catttaaata atatactcct gaacggcgac gcgaatcatt ttttcctaac
    35101 ttccagagta aaacgtccat ttcatcccat ttacggaact tcgtttgtgg aattgtacaa
    35161 tatctatttt ttatagtgag atcgcttttc atatttaaaa aatcctttct tcccaacagc
    35221 tatatgattc cgtactcgcg atatggtgct tccatgttat atttttataa ttaagaacaa
    35281 tgacctcttc cggttgatta cccgcatgtg taattgagtg tggataacaa gtcagaatat
    35341 tcgataaata tgccccttgc aattcaattg tgtaaaacct ttcaatacaa ccgccagaat
    35401 taattctata aaaattaaat acagctgata actcttcatt ttcagaaaga gcagcattta
    35461 ataaaggaga ggatttatct attggcttgt aaaattttat gggatgatgc actacattat
    35521 attctcgact taaagaatgg tcgaactgta aaacaaaaat ttcatccaca tgatttattt
    35581 gatatttatt accaatagat gc
//
LOCUS       DAZGTJ010000046        35173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000046 DAZGTJ010000000
VERSION     DAZGTJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.046"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..2396
                     /gene="polB"
                     /locus_tag="ACHK8E_002777"
     CDS             45..2396
                     /gene="polB"
                     /locus_tag="ACHK8E_002777"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP0345021.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2560..5466
                     /gene="rapA"
                     /locus_tag="ACHK8E_002778"
     CDS             2560..5466
                     /gene="rapA"
                     /locus_tag="ACHK8E_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP0345022.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5478..6137
                     /gene="rluA"
                     /locus_tag="ACHK8E_002779"
     CDS             5478..6137
                     /gene="rluA"
                     /locus_tag="ACHK8E_002779"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP0345023.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6255..7070)
                     /gene="djlA"
                     /locus_tag="ACHK8E_002780"
     CDS             complement(6255..7070)
                     /gene="djlA"
                     /locus_tag="ACHK8E_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP0345024.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7325..9679
                     /gene="lptD"
                     /locus_tag="ACHK8E_002781"
     CDS             7325..9679
                     /gene="lptD"
                     /locus_tag="ACHK8E_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP0345025.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9732..11018
                     /gene="surA"
                     /locus_tag="ACHK8E_002782"
     CDS             9732..11018
                     /gene="surA"
                     /locus_tag="ACHK8E_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP0345026.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11018..12007
                     /gene="pdxA"
                     /locus_tag="ACHK8E_002783"
     CDS             11018..12007
                     /gene="pdxA"
                     /locus_tag="ACHK8E_002783"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP0345027.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12004..12825
                     /gene="rsmA"
                     /locus_tag="ACHK8E_002784"
     CDS             12004..12825
                     /gene="rsmA"
                     /locus_tag="ACHK8E_002784"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP0345028.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12828..13205
                     /gene="apaG"
                     /locus_tag="ACHK8E_002785"
     CDS             12828..13205
                     /gene="apaG"
                     /locus_tag="ACHK8E_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP0345029.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13212..14054
                     /gene="apaH"
                     /locus_tag="ACHK8E_002786"
     CDS             13212..14054
                     /gene="apaH"
                     /locus_tag="ACHK8E_002786"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP0345030.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14132..14611)
                     /gene="folA"
                     /locus_tag="ACHK8E_002787"
     CDS             complement(14132..14611)
                     /gene="folA"
                     /locus_tag="ACHK8E_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP0345031.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14803..16665)
                     /gene="kefC"
                     /locus_tag="ACHK8E_002788"
     CDS             complement(14803..16665)
                     /gene="kefC"
                     /locus_tag="ACHK8E_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP0345032.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16658..17188)
                     /gene="kefF"
                     /locus_tag="ACHK8E_002789"
     CDS             complement(16658..17188)
                     /gene="kefF"
                     /locus_tag="ACHK8E_002789"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP0345033.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17296..18627)
                     /gene="yaaU"
                     /locus_tag="ACHK8E_002790"
     CDS             complement(17296..18627)
                     /gene="yaaU"
                     /locus_tag="ACHK8E_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345034.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18686..18973)
                     /gene="fixX"
                     /locus_tag="ACHK8E_002791"
     CDS             complement(18686..18973)
                     /gene="fixX"
                     /locus_tag="ACHK8E_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP0345035.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18970..20256)
                     /gene="fixC"
                     /locus_tag="ACHK8E_002792"
     CDS             complement(18970..20256)
                     /gene="fixC"
                     /locus_tag="ACHK8E_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0345036.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20307..21248)
                     /gene="fixB"
                     /locus_tag="ACHK8E_002793"
     CDS             complement(20307..21248)
                     /gene="fixB"
                     /locus_tag="ACHK8E_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0345037.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21263..22033)
                     /gene="fixA"
                     /locus_tag="ACHK8E_002794"
     CDS             complement(21263..22033)
                     /gene="fixA"
                     /locus_tag="ACHK8E_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP0345038.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            22506..24020
                     /gene="caiT"
                     /locus_tag="ACHK8E_002795"
     CDS             22506..24020
                     /gene="caiT"
                     /locus_tag="ACHK8E_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP0345039.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24051..25193
                     /gene="caiA"
                     /locus_tag="ACHK8E_002796"
     CDS             24051..25193
                     /gene="caiA"
                     /locus_tag="ACHK8E_002796"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP0345040.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            25321..26538
                     /gene="caiB"
                     /locus_tag="ACHK8E_002797"
     CDS             25321..26538
                     /gene="caiB"
                     /locus_tag="ACHK8E_002797"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP0345041.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            26611..28164
                     /gene="caiC"
                     /locus_tag="ACHK8E_002798"
     CDS             26611..28164
                     /gene="caiC"
                     /locus_tag="ACHK8E_002798"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP0345042.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            28273..29058
                     /gene="caiD"
                     /locus_tag="ACHK8E_002799"
     CDS             28273..29058
                     /gene="caiD"
                     /locus_tag="ACHK8E_002799"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP0345043.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29064..29654
                     /gene="caiE"
                     /locus_tag="ACHK8E_002800"
     CDS             29064..29654
                     /gene="caiE"
                     /locus_tag="ACHK8E_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP0345044.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(29773..30168)
                     /gene="caiF"
                     /locus_tag="ACHK8E_002801"
     CDS             complement(29773..30168)
                     /gene="caiF"
                     /locus_tag="ACHK8E_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP0345045.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(30429..33650)
                     /gene="carB"
                     /locus_tag="ACHK8E_002802"
     CDS             complement(30429..33650)
                     /gene="carB"
                     /locus_tag="ACHK8E_002802"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP0345046.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(33668..34816)
                     /gene="carA"
                     /locus_tag="ACHK8E_002803"
     CDS             complement(33668..34816)
                     /gene="carA"
                     /locus_tag="ACHK8E_002803"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP0345047.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         8445 a         9499 c         9188 g         8041 t
ORIGIN      
        1 aaaccaggct ataatcaagc ctggtttttt attggatttt cagcgtggcg caggcaggtt
       61 ttatcttaac ccgacactgg cgggataccc cgcaaggaac cgaagtttcc ttttggttag
      121 cgacggacaa cgggccgttg caggttacgc ttgcgccgca agagtccgtg gcgtttattc
      181 ccgccgatca ggttccccgc gcccagcata ttttgcaggg tgagcaaggc tttcgcctga
      241 cgccgctggc gttaaaggat tttcatcgcc agccggtgta tggcctttac tgtcgcgccc
      301 atcgccagtt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc gtctacgagg
      361 ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca ccggtatggg
      421 tcgagggtga tatacgcaat ggcgctatcg ttaatgcccg tctgaaaccg catcccgact
      481 accgtccgcc gctcaagtgg gtttctattg atattgaaac cacccgccac ggtgagctgt
      541 actgcatcgg cctggaaggt tgcgggcagc gcatcgttta tatgctgggg ccagagaatg
      601 gcgacgcctc cgcgctcgat tttgaactgg agtacgtcgc cagccgcccg cagctactgg
      661 aaaaactcaa cgcctggttt gcgactcacg atcccgatgt gatcatcggc tggaacgtgg
      721 tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtatt ccgctgcggc
      781 tggggcgcga taacagcgag ctggagtggc gcgagcacgg ctttaaaaac ggtgtgtttt
      841 ttgctcaggt taaaggtcgg ctaattatcg acggtatcga ggcactgaaa tccgcgttct
      901 ggaatttctc ttcattctcg ctggaaaccg tcgctcagga gttattaggc gaaggaaaat
      961 ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc gaagataagc
     1021 ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc ttccacaaaa
     1081 ctgaaatcat gccgtttttg ctcgaacggg cgacggtgaa cggcctgccg gtagatcgac
     1141 acggcggttc ggtggcagcg tttggtcatc tctattttcc ccgtatgcat cgcgctggtt
     1201 atgtcgcgcc taatctcggc gaagtgccgc cgcacgccag ccctggcggc tacgtgatgg
     1261 attcgcgacc tgggctttat gattcggtgc tggtgctgga ctacaaaagc ctgtacccgt
     1321 cgatcatccg cacctttctg attgatcccg tcgggctggt ggaaggcatg gcgcagcctg
     1381 atccagagca cagcaccgaa ggctttctcg atgcctggtt ctcgcgagaa aaacattgcc
     1441 tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt cagggtaata
     1501 agccgctttc gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg cttggcacca
     1561 ccgcctgccg cttcttcgat ccgcgcctgg cgtcgtcgat cactatgcgt ggtcatcaga
     1621 tcatgcggca aaccaaagcg ttgattgaag cgcagggcta cgacgtgatc tacggcgata
     1681 ccgactcaac gtttgtctgg ttgaaaggcg cacattcgga agaagaagcg gcgaaaattg
     1741 gtcgtgcact ggtgcagcac gttaacgcct ggtgggcaga aacgctgcaa aaacaacggc
     1801 taaccagcgc actggaactg gagtatgaaa cccatttctg ccgttttctg atgccaacca
     1861 ttcgcggagc cgataccggc agcaaaaagc gctacgccgg actgattcag gagggcgaca
     1921 aacagcggat ggtatttaaa gggctggaaa ccgtgcgcac cgactggacg ccgctggccc
     1981 agcagtttca gcaggagctg tacctgcgta tcttccgcaa cgagccatat caggaatacg
     2041 tgcgcgaaac catcgacaag ctgatggcgg gcgaactgga tgcgcggctg gtttaccgta
     2101 aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt tccgcctcat gtacgcgccg
     2161 ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat cagaaccgcg
     2221 gctccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac caacgttcac
     2281 cgctggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag ggaatactcc
     2341 cttttattga ggataatttt gctacactta tgaccgggca acttgggcta ttttgagcaa
     2401 aaaaagagtt cgccagatac cattttgatg cgtgacgaat gctttgccat ccagtaccat
     2461 agcgcccttt ccattcctgg acctgaataa caccactacc tcataagcac ggtagcgggt
     2521 ggttattgcc tgcaattaaa gatatagagc cgaacacata tgccttttac acttggtcaa
     2581 cgctggatca gcgatacaga aagcgaattg ggacttggaa ccgttgtcgc ggtggatgcg
     2641 cgaactgtca ctttactttt cccatctact ggtgaaaacc gtctgtacgc acgcagtgat
     2701 tcccccgtga cccgcgtgat gttcaaccct ggtgatacta ttaccagcca tgacggctgg
     2761 cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc tgacctatat cggtactcgc
     2821 ctggatactg aagagtccgg cgtagccctg cgtgaagttt tccttgatag caaactggtg
     2881 ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg accgtatgga ccgctttgcg
     2941 ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc gtatgccgta cagcggcctg
     3001 cgcggtcagc gtaccagcct gatcccgcac cagctcaaca tcgctcatga tgttggccgc
     3061 cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt tagggaaaac cattgaagcc
     3121 gggatgatcc tgcatcagca actgctctct ggcgctgctg aacgtgtgct gattatcgtc
     3181 ccggaaacct tacagcatca gtggctggta gaaatgctgc gccgtttcaa cctgcgcttt
     3241 gctctgtttg atgatgagcg ttatgccgaa gctcagcacg atgcttacaa cccgttcgat
     3301 accgaacagc tggtgatttg ctcgctggat tttgctcgtc gtagcaaaca gcgtctggaa
     3361 catctctgtg aagccgaatg ggatctgctg gtggtcgatg aagcccatca cctggtgtgg
     3421 agcgaagatg cgccgagccg cgaatatcag gccattgaac aactggcaga gcacgtgccg
     3481 ggcgttctgc tgctgaccgc aaccccggaa cagctgggga tggaaagcca cttcgcccgt
     3541 ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc aattcgttga agagcagaaa
     3601 aattatcgtc cggttgcgga cgccgttgcc atgctgctgg caggtaacaa actgagcaat
     3661 gatgaactga acatgctcgg tgagatgatc ggcgagcagg atatcgagcc gctgttgcaa
     3721 gcagcaaaca gcgacagcga agatgcccag agcgcccgtc aggaactggt ttcgatgctg
     3781 atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca cgcgtaacgg tgtgaaaggc
     3841 ttcccgaaac gcgagctgca tactattaag ctgccactgc cgacgcagta tcagaccgct
     3901 attaaagtct ccggcattat gggcgcacgt aaaagtgcgg aagatcgcgc tcgcgatatg
     3961 ctctacccgg agcgtattta tcaggaattt gaaggcgata acgccacctg gtggaacttc
     4021 gatccgcgcg ttgaatggct gatgggctac ctgaccagcc atcgctctca gaaagtgctg
     4081 gtgatctgcg ccaaagctgc cactgcgctg caactggagc aagtactgcg cgaacgtgaa
     4141 ggtatccgcg ctgctgtgtt ccacgaaggc atgtcgatta tcgaacgtga ccgcgctgcc
     4201 gcctggtttg ctgaagaaga caccggtgcg caggtactgc tgtgctcgga aatcggttct
     4261 gaaggacgta acttccagtt cgccagccac atggtgatgt ttgacctgcc attcaacccg
     4321 gatctgctgg agcagcgtat tggtcgtctg gatcgtatcg gtcaggcgca cgatattcag
     4381 atccatgtgc cttatctgga aaaaactgct cagtcggtgc tggtgcgctg gtatcacgaa
     4441 ggtctggatg cattcgagca cacctgcccg accggacgca ctatttacga tagtgtatac
     4501 aacgatctga ttaactatct ggcttcaccg gatcaaaccg aaggctttga cgatctgatc
     4561 aaaaactgcc gcgagcaaca tgaagcgctg aaagcacagc tggaacaagg tcgtgaccgc
     4621 ctgctggaaa tccattccaa cggtggcgaa aaagcccagg cactggcaga aagcattgaa
     4681 gagcaggatg acgataccaa cctgatcgcc ttcgccatga acctgttcga tattatcggt
     4741 atcaatcagg acgatcgcgg cgacaacatg atcgtgctga cgccgtccga tcatatgctg
     4801 gtgccggact tccctggcct gtcggaagat ggcatcacca tcaccttcga ccgtgaagtg
     4861 gcgctggcgc gtgaagatgc acagtttatt acctgggagc atccgctgat ccgcaacggt
     4921 ctggatctga tcctttctgg cgataccggt agcagcacga tttcactgtt gaaaaacaaa
     4981 gcgttgccgg taggtacgct gttggtggaa ctgatttatg tggttgaagc ccaggctccg
     5041 aagcagttgc agctcaaccg cttcctgcca ccgacgccgg tacgtatgct gctggataaa
     5101 aacggcaaca acctggcggc gcaggtagag tttgaaacct ttaaccgcca gcttaacgcg
     5161 gttaaccgtc acaccggcag caagctggtt aacgccgtgc agcaggatgt tcacgctatc
     5221 cttcagctgg gggaagcgca gatcgagaaa tctgcccgtg cattgattga tgccgcacgt
     5281 aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc tggaagcgct gcgtgcagtt
     5341 aacccgaaca ttcgtgacga cgaactgacc gccattgaga gcaaccgtca gcaggtaatg
     5401 gaaagcctgg atcaggcagg ctggcgtctg gatgccctgc gtttgatcgt tgtgacgcat
     5461 cagtaacgga gccgaagatg gggatggaaa actacaatcc gccgcaggaa ccctggctgg
     5521 ttgtcctgta tcaggatgac catattatgg tggtcaacaa gccgagcggt ttgttgtctg
     5581 tgccgggtcg tctggaagag cacaaagaca gcgtgatgac gcgcattcag cgtgattatc
     5641 cgcaggcaga atcggtgcat cgtctggata tggctaccag cggcgtgatt gtagtggcgc
     5701 tgaccaaagc cgcggagcgg gagttaaaac gccagttccg cgagcgcgag cctaaaaaac
     5761 agtatgtggc ccgcgtctgg gggcatccat cccctgcgga aggtctggtg gatctgccgc
     5821 tgatttgcga ctggccaaac cgcccgaaac agaaagtctg ttacgaaaca ggtaaacctg
     5881 cgcagacgga gtatgaagtg gtggaatatg cggcggataa cacggcaaga gtggtgttaa
     5941 aaccgattac cgggcgttcg catcagctgc gtgtgcatat gctggcgctg ggtcatccga
     6001 ttctcggcga tcgtttttat gcatcaccag aagcgagagc aatggcacca cgtttgttgc
     6061 tacatgcaga gatgctgacg attacccatc cggcgtatgg caatagtatg acgtttaaag
     6121 caccagcgga tttttaatag tcacgctggc gaagttcggt aggccggaga aggcgttcac
     6181 gccgcatccg gcaattttgc gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc
     6241 ctacacgttg aaacttattt aaaccctttc tgcagcttaa tcaattcata ggcctgctga
     6301 atttcctgcg ctttttgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
     6361 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
     6421 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgttgg gccacgctgc
     6481 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
     6541 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
     6601 atgacataca gcacagcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
     6661 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
     6721 tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa cgcgttttgc
     6781 gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcaactggct ggcgatatga
     6841 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
     6901 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
     6961 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
     7021 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
     7081 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
     7141 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
     7201 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacgag
     7261 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
     7321 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
     7381 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
     7441 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctgaccacgc
     7501 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
     7561 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcgccag gacaaccaga
     7621 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
     7681 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggagggtga
     7741 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
     7801 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
     7861 ctggagcgtg gtaggcagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
     7921 gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt tgcagttgcc
     7981 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
     8041 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
     8101 cacgccgcat tatatgcatc gtcgtggcaa tatcatgtgg gagaacgaat tccgctacct
     8161 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
     8221 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
     8281 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
     8341 cttcaatgat tttgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
     8401 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
     8461 cttcagcgag cagaacacca gtagctactc ggcagagccg cagttagacg ttaactacta
     8521 ccagaatgat gttggtccgt ttgatacacg catttacggc caggcagtgc actttgttaa
     8581 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
     8641 gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca
     8701 gcaaactaat cttgactggt ataactccag aaacacaacc aagctggacg aatccgttaa
     8761 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
     8821 gctggcgccg ggttataccc aaacgctgga accgcgcgcg cagtatttat acgtgccgta
     8881 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
     8941 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
     9001 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
     9061 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
     9121 tgacgacaaa acgggctcac tggtgtgggc aggcgatact tactggcgta tctcagagcg
     9181 ttggggattg cgtggcggaa ttcagtacga tacacgtctg gataacgtag cgaccagtaa
     9241 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc
     9301 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
     9361 gaatggtatt tcgcaggtag gtgccgtcgc cagctggcca attgccgatc gttggtcaat
     9421 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
     9481 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
     9541 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
     9601 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
     9661 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
     9721 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
     9781 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
     9841 tgctggaaag cgacgttgat ggattaatgc aatcggtaaa actgaacgct gctcaggcaa
     9901 ggcagcagct tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
     9961 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    10021 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    10081 gtctggctta cgatggtctg aactacaaca cctatcgtaa ccagatccgc aaagagatga
    10141 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    10201 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    10261 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaagtgaac gaagcggaaa
    10321 gccaggcgcg tgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    10381 ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc
    10441 aggagttgcc ggggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    10501 gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg cgcggcgaaa
    10561 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    10621 tcatgactga cgaacaggcc cgcgtgaaac tggaacagat tgctgccgat atcaagagtg
    10681 gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc tctgctaacc
    10741 agggcggtga tctcggttgg gctacaccag atattttcga tccggccttc cgtgacgccc
    10801 tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    10861 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    10921 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    10981 aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    11041 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    11101 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    11161 gatgctcggt ttgccgctca ccctccgtcc ttattccccc aactcccctg cacaaccgca
    11221 aactgcgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    11281 gttagcggtt gaaaatgggc attacgtagt ggagacgctg gcgcgagcgt gtgatggctg
    11341 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    11401 cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    11461 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    11521 gctgcgcgat atcgcagatg ctatcacccc tgcgcttttg cacgaagtga ttgctatttt
    11581 gcatcacgat ttgcggacca aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    11641 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    11701 ggtgctcgac aagctgcggg cgcaggggat gaaactcaac gggccgctgc ctgccgatac
    11761 cctgtttcag ccgaaatatc ttgataacgc cgacgcggtg ctggcgatgt accacgatca
    11821 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    11881 gccctttatt cgcacatcag tggaccacgg taccgcgctt gaactggcgg gacgtggcaa
    11941 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    12001 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    12061 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca gaagggccag
    12121 gcgatggtcg aaatcggccc cggtctggcg gcattgaccg aaccggtcgg cgaacgtctg
    12181 gataagctca cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    12241 ttcttaggcc cgaaactgac tatttatcag caggatgcga tgacctttaa ctttggtgaa
    12301 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    12361 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    12421 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaggc gtatggtcga
    12481 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tgctggaagt accgccgtca
    12541 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg
    12601 atgcctcacc cggttaaaga tgttcgcgtg ttgagccgca tcaccaccga agcctttaac
    12661 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg
    12721 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    12781 atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc
    12841 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    12901 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    12961 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    13021 aaggagtggt tggcgtccag ccacttatcg cacctggcga agagtaccag tacaccagcg
    13081 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    13141 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    13201 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    13261 tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggcg
    13321 atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg
    13381 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta tttgccggga
    13441 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    13501 agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg
    13561 tgatggccca cgccgggatc acgccacagt gggatctaca gaccgccaaa gagtgcgcgc
    13621 gcgatgtaga agcggtgctg tcgagcgact cctacccctt cttccttgat gccatgtacg
    13681 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cggtttatca
    13741 ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat atgtacagca
    13801 aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg gtttgcgatt cctggccctg
    13861 tcgctgaaga gtacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    13921 cggaaggtat ttacgcgctg gatactggct gctgctgggg tggtacatta acctgcctgc
    13981 gctgggaaga taaacactat tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag
    14041 cggcggcgtc ttaaacacag cctgatacag gaaggccgga taagacgcga ccggcgtcgc
    14101 atccggcact agccgtaaat tctatacaaa cttaccgccg ctccagaatc tcaaagcaat
    14161 agctgtgaga gttctgcgca tcagcgtcgt ggaattcgct gaataccgat tcccagtcat
    14221 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
    14281 acagtttttg cgctttgggc aggaactgct cataaacgcg accgccgcca atcaccatga
    14341 tttctggtac gtcaccacac gccgcaatgg cttcatccac cgacttcacc cacgttacgc
    14401 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
    14461 cgatggattc ccaggtatgg cggcccataa tcacgggttt atttaaagtg ttgcgtttaa
    14521 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacacgat
    14581 ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc
    14641 cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt
    14701 tatattctgc tggcgagtcc acgctctccc actggactcg ccgcattaca atgaaataaa
    14761 aacaattagt tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt
    14821 ttccggttca tccgccatgt tgccggtatg tttaccttct tcggttccct gccagccatg
    14881 tcgttgaatt aatgacagat gttcgcggtc ctcggtaatg atctcactta acatcgcgct
    14941 ggtgcgttta tagaccgccg cgcgggcttt ggtgtcgttc tcaaccattg ccatctcttc
    15001 caccatctga atattaaagc ggcggaacac atcggcgcgt tctcgcgctt catacggccc
    15061 cagacctaaa ctttccagtg ccagacgccc ggttttcagc gcaccttcaa aggtttcacg
    15121 ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg
    15181 ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt gcaggttggt
    15241 ttgcggatcg tcgatagcgt taatcagcac ttccgctttc gccgctccgg cagattccag
    15301 taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac gcaaggtttc
    15361 gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga gcagtaaacg
    15421 tccggtgatc tgcccaaaac gaccgaatcc ggcgatgatt acccgcggct gttcttcgtc
    15481 gatctcatcg gcttcacgcg cctcttcact agaagattgc tcaaggcgat tgaggatcac
    15541 cagcagaatc ggcgttgccg ccatcgacag cgccaccgcc agggtcagcg atttcgccca
    15601 ctccggctcc agcacattcg ccatctgcgc cgcaccaaat actacaaagg caaactcact
    15661 accctgccct aatagcaccg caaaccagcg acgctgttta tttggcacct gcaacggccg
    15721 ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat
    15781 gcgcaacgga ttttcaagca gcgtgccaaa gtctatcgac atgccaacgc cgatgaaaaa
    15841 cagccccagc agcaaacctt taaatggttc gatatcgctt tccagcgcat gacggtattc
    15901 cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc
    15961 ttccagcagc aaaccaaagc caaacacgag gaataacgcc acggcactaa acacttcccg
    16021 caagccagag cgggcaacaa aacgcagtgc cggacgcgtg acatagcgcc ccagcaatac
    16081 caccagcacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt
    16141 cgaggcactg ctcgccgcca gtagcgggat catcgccacc agcgggatcg ccgcgatatc
    16201 ctggaacagc agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttc
    16261 attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc
    16321 cgcgacctgc cagcgcagcc caagcaacat acagaacagc cccagcaggc cgccgcaaat
    16381 caccatctgc aatgcgccgc cgccgaacac cgccgcacgc agcttccaca gcctttgtgg
    16441 atcgagttcg aggccgataa taaacagcat cagcaccacg ccaatctcgg caaagtgcag
    16501 aatagattcg gcatcggtca ccagtcgcag cccccacggg ccaataatgc agccggcgat
    16561 caggtagcca agtaccgctc ccagaccgag acgtaccgca atgggaacaa tcagcgccgc
    16621 cgaaccgaga taaatcagcg cctgaataag cgtatggcta tccatgatgt gcctcctgcc
    16681 attccagcag acgttgctta tagtgccgcg cctgcccttc gagggtttcg tcgtcacaaa
    16741 taaaggtgca gtgcatggca aacggtggca gccagttcag cccgcagtag attgccgtcg
    16801 cctgtagcgg ctgtgacagc acatcaaagc ccggatgcgc accaatctca aaatggcttt
    16861 ccccaccgcc ggtcgtcacc gcccacagta aatgtttgcc atgcagcgcc gtgccgccgt
    16921 gaccgtaagc ccagccgtgc gagaagactt tatcgatcca aagtttaaga agcggaggaa
    16981 tgctgtacca ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct
    17041 gctcggcggc aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga
    17101 cgccttccag cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat
    17161 acggatgcgc ataaattata agaatcatag gtagcccgcg tgattacgcc tttgttttcc
    17221 cacaaagtgt agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag
    17281 agaaatctga acaaattacc ccattctctg cccgcggatc gtcatattgc tggtctgcgc
    17341 cagtgacatc cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga
    17401 gatacccgcc cccattagca tcgtgttact gataccgtaa ttattgataa agatcggcag
    17461 tgcccaggtc gaaacaatag tgccaatacg gcttaaggac ataatcacgc ccacggcaga
    17521 ggcgcggata tccgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc
    17581 gccagagaaa aaggcataca ccgcaaatgc catcactacc agccagatcc ccatatccgg
    17641 gatcagcccc aaaaccgcca gcgccagcgt catcatggca aagctgccaa tcaacaatgg
    17701 acgccgtccg gcagtgttta gccacagcat cggtggaata cagccgagca taaagaacag
    17761 gctaatcacc acgttcccca gtgccgcgtt tttgccaaca cccaatccca acaaaccaac
    17821 gatttgcggg ccaaaggtgt agatggcgaa catcgggatc acctggcagg tccagatggc
    17881 ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg
    17941 ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac agtttgatca tcatctcttc
    18001 gcactctttt actcgccctt tgcgtaacaa ccagcgggga gattcaggca gttcgaatcg
    18061 accaatcaaa atcaacaaac aggggatcgc tgcgctaccc agcatccagc gccaaccacc
    18121 ttccacatca taaagccagt aaccgaccag atcggcgcag gtcgcgccga cataccacat
    18181 cgcggcaata aagctgatgg aaaacgcccg ctgacgggta ctggagaact cagtgatcat
    18241 cgaagtggcg atgggataat ctgcaccgat gacaatgccg atgagtaccc gcatcactaa
    18301 cagttcgacg ggggatgaga caaacatcgt cgccaccgat atcacgccga tggcgatgat
    18361 atcaatgagg aacattttgc gccgtccgac tttatcggag atatagccaa acagcgatgt
    18421 gccaacgaac agcccggcga gcgttcccgc gcccagcaag ccaatccagt cagcgtccag
    18481 tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc
    18541 cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg tgaatagagg agaatttaag
    18601 atcatcaaag tttctggacg gttgcatggg ttattcctgt ttttttgcaa tgagagtcag
    18661 tacggcatta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    18721 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    18781 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    18841 cggggcacgc tttcaccagc agctccaggg cctgtttatc ggcatcggcc ttcacaacga
    18901 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    18961 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc
    19021 cactttcttg ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat
    19081 ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg tagccgctaa acatgcgtgg
    19141 gttatcaagg aacgccggta gtttctggta catgcgcata tcgcgcagcg ggccactctc
    19201 aagatgctga cgatattccg cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    19261 aagcacagtt tttgctgctg cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    19321 aaaaccgagg ttcatacaca ttccggcagc atcaccggca atcaatacgc cgtcaccaac
    19381 caactccggc agcatgttga tacctgcttc cggcactacg tgagcggaat attccaccag
    19441 cttgccgccc gcgatcagcg gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg
    19501 cggcaccgat ttttttgcgt catgcagatg atgcaaacca caaaccagcc ccagcgacag
    19561 ggtgttttca ttggtataaa gaaagccgcc gcccatcagg ccatcagtgg gggaacccgc
    19621 aaacaggcaa gccgcaccct gattaccctg caactgaaaa cggtcttcga taaccgactt
    19681 cggtaattcg atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc
    19741 catccccagc ttttcggcaa ggatggagtt caccccgtca gcaaggatca ccgttttcgc
    19801 ttcaattaca tcgccatcgg cttctacgcc gacaactttg ccatcgcgct gtacgaggtt
    19861 atcgacacga atcccggtaa ttaactgcgc acccgcttct tcggcctgct ccatcagcca
    19921 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggtgagg tttcgtcacc
    19981 gttgcagtag tccatagtca tcgcagactt ttccgtcata aacgcgagtt tttcatgggt
    20041 gatcaggcgt tctacggggg cggagtcagc gaaaccagga ataatgtgtt ccagactgtg
    20101 ggcatagaga cgcccgccgg tgacgttctt ggcaccagcg gaattgcctc gctcgataac
    20161 taacacttgc gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagccctgc
    20221 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcaggg
    20281 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag tgccggaagg
    20341 atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    20401 tctttgttga tggcgaaaat ggtttgcgac gcattagcgc caaccatgtg ctggatctgc
    20461 ccggagatcc ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca
    20521 tagcgttcgt gctccatcca tttttcgttt tccgccaccg gacgagaaca ggccaactcc
    20581 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    20641 cgaccaacgc tgaccaccag gcgggctttg tcgagatcga cgctgttgct ctgacgcgcc
    20701 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    20761 ccagtgcgtg acgcgtctgg ctgagccaca tcgaacgtgc cgctgctgat ggtcagaacc
    20821 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac cgccgtaaac catgtgtttc
    20881 actgtcgctt taccgtcctg cacgctaaca gcgctggcat cgttagacac cgccgcgtta
    20941 aggcggtaac ccagtttcgc cgccagtaat ttgccgcgac gggtgtttgg caacagcacc
    21001 agaccgtctg cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc
    21061 atccgctcgt ccggtttgcc gcttaatttc cagacatgat tagcgccgag ctggattgcc
    21121 tgtgcgccgt cggcatcatt gaggacgaac gtgttgattt gattagctaa agcctgcgca
    21181 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    21241 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    21301 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ctcgcgctgt ttcggcgcgg
    21361 caacctgttg ttctgaccag gctgcctctg cgttaaaacc gatatccgcc gccgaccata
    21421 cctggactgg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    21481 tgatatcagt ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tctgtttcat
    21541 cttccagttc gcgctcaacg gtgagggtat ctgcagtcag ggagataatt ttgctgacgc
    21601 cgttaactgc cggaatatta aggatttcgc ctaccagcaa accaacctgc tgggcataaa
    21661 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctga gcggctgcag
    21721 ccagtacgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    21781 tcagttcatc cggaccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    21841 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    21901 ccgcttcaat agcgttgaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    21961 acgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    22021 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    22081 taattacaga tatcacgccc tttaatttgg aaataaaact ttaatcacca atattgaaaa
    22141 tgtcacacgc agataaatcc aactttcaat attgttaagc tcctcaccaa tattgaaaac
    22201 atggcgtaga aaaaagaaat tttcaatatt gttttatgga tcaccaatat tgaaagtcag
    22261 atgctatttt tttactcaca acagaacata acaaactgat taataatcaa aattaacgaa
    22321 aaaatgccct ctattcaccc gaaacaaaaa tgtgataaca atcacagaat gcagcttatt
    22381 gaatacccat tatgagttag ccattaacgc gtccacgagg ttaataataa ttatattaaa
    22441 tgttaacaaa aataaaacaa acgggaacgc aaaataaatg ttcgttttca cagtggaatt
    22501 aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccgt
    22561 taataatcgt cggcatactt tgttggctta cagtcagaga tctggatgcg gcgaatgtcg
    22621 ttattaatgc tgtattcagt tacgtcacca atgtatgggg atgggcattt gaatggtata
    22681 tggtggtgat gcttttcggc tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt
    22741 taggtaacga accaccagaa tttagtaccg ccagttggat ctttatgatg ttcgcctcct
    22801 gtacgtctgc tgccgtactg ttctggggat cgattgagat ctactactac atctccaccc
    22861 cgccgtttgg cttagaaccg aactcaacag gcgcgaaaga gttggggctg gcttacagct
    22921 tgttccactg gggacctctg ccgtgggcca cttacagctt cctttcagtc gccttcgctt
    22981 acttcttctt tgtccgcaaa atggaagtga ttcgccccag ctccaccctg gtgccgctgg
    23041 taggtgaaaa acacgccaaa gggttgttcg gcactatcgt cgacaacttc tatctcgtcg
    23101 ccttgatctt cgcgatgggt accagtctgg gccttgccac gccgctggtg accgagtgta
    23161 tgcaatggtt gtttggcatt ccgcataccc tgcaactgga cgctatcatc attacctgct
    23221 ggattatcct caacgccatt tgcgtcgctt gcggtctgca aaaaggggta cgtatcgcca
    23281 gtgacgtgcg tagttacctg agcttcctga tgctgggttg ggtgttcatc gtcagcggtg
    23341 ccagcttcat catgaactac ttcaccgatt cggtggggat gttgctgatg tatctgccgc
    23401 gcatgttgtt ctataccgat cccatcgcta aaggcggctt cccgcagggc tggaccgtgt
    23461 tctactgggc atggtgggtg atttacgcta tccagatgag catcttcctc gcccgcatct
    23521 cccgtggtcg taccgtgcgt gaactgtgct tcggcatggt gctggggctg acagcgtcaa
    23581 cctggatcct gtggactgta ctcggtagta acactctgct gttgatagat aaaaacatca
    23641 tcaacattcc aaatctgatc gaacagtacg gtgtggcgcg cgccatcatc gaaacctggg
    23701 ccgctctgcc actcagcacc gccaccatgt ggggcttctt catcctctgc tttattgcca
    23761 ccgttacgct ggttaacgcc tgctcttata ccctggcgat gtccacttgc cgcgaagtac
    23821 gcgatggtga agaaccaccg ctgctggtgc gtatcggctg gtcaattctg gttggcatta
    23881 tcggtattgt tctgctggcg ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg
    23941 gaggatgccc gctgttcttc gtcaacatta tggtgacgct ctcctttatt aaagacgcga
    24001 aacagaactg gaaagattaa ttaaccccca aaatatcaag aggttgaaag atggatttta
    24061 atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa
    24121 actgggaggc ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca
    24181 aagcactggc ggatatgggt atcgacagtc tgctgattcc tgaagagcac ggtggtcttg
    24241 acgcgggttt tgttactctc gccgccgtgt ggatggagct gggacgtctg ggggcaccaa
    24301 cctatgtgct gtaccagttg ccgggcgggt tcaatacctt cctgcgcgaa ggcacacaag
    24361 agcagatcga caagattatg gctttccgcg gcaccggtaa gcagatgtgg aactcagcga
    24421 ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat acccgtagaa
    24481 atggtaagat ttatcttaat ggtagtaagt gttttattac cagcagcgcc tacaccccgt
    24541 acatcgtggt gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt
    24601 ttgttgatat gagcaaaccg ggcatcaaag tgaccaaact tgagaagctc ggtctgcgta
    24661 tggatagctg ctgtgaaatc acttttgacg acgtggaact ggacgagaaa gacatgttcg
    24721 gtcgggaagg taacggattt aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg
    24781 tagccctgac caactacggt acggcgatgt gcgcctttga agatgcggcg cgctacgcca
    24841 accagcgcgt gcagtttggc gaggctattg gtcgtttcca gttgattcag gaaaaattcg
    24901 cccacatggc gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag
    24961 cagacaacgg caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg
    25021 cggcatttga agtggtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca
    25081 accaccgcat cagccgcttc tggcgtgacc tgcgtgtaga ccgcgtttcc ggaggatctg
    25141 acgaaatgca gatcctgacg ctgggtcgcg cggtgctgaa gcaataccgt taagtccgtg
    25201 cgctgcctga tgcgacgctg acgcgtctta tcaggcctac gggagcacga agatggccgg
    25261 ataaggcgtt tacgccgcat ccggcagtca tgcgctacat aaatttttca ggagctaata
    25321 atggatcatc tacccatgcc gaaattcggg ccgctggccg gattacgcgt tgtcttctcc
    25381 ggtatcgaaa tcgccgggcc gttcgccgga caaatgttcg cagaatgggg ggcggaagtt
    25441 atctggatcg agaacgtcgc ctgggccgac accattcgcg ttcaaccgaa ctacccgcag
    25501 ctctcccgcc gcaatttgca tgcgctgtcg ttaaatattt tcaaagatga agggcgcgaa
    25561 gcgtttctga aattaatgga aaccaccgat atcttcatcg aagccagtaa aggtccggcc
    25621 tttgcccgtc gtggcattac cgatgaagtc ctgtggcagc acaacccaaa actggttatc
    25681 gctcaccttt ccggttttgg tcagtatggc accgaggagt acaccaatct tccggcctat
    25741 aacactatcg cccaggcctt cagtggttac ctgattcaga acggtgatgt tgaccagcca
    25801 atgcctgcct tcccgtatac cgccgattac ttttctggcc tgaccgccac cacggcggcg
    25861 cttgcagcac tgcataaagt gcgtgaaacc ggcaaaggcg aaagcatcga catcgccatg
    25921 tatgaagtga tgctgcgtat gggccagtac ttcatgatgg attacttcaa cggcggcgaa
    25981 atgtgcccgc gcatgaccaa aggtaaagat ccctactacg ccggttgcgg tctgtataaa
    26041 tgcgccgacg gctacatcgt gatggaactg gtgggcatta ctcaaattgc agagtgcttt
    26101 aaagatattg gcctcgcaca cctgctcggt acgccggaaa tcccggaagg cactcagctt
    26161 atccaccgta tcgaatgccc ttacggccca ctggtagaag agaaactcga tgcctggctg
    26221 gcggcacata ccatcgcaga agttaaagaa cgctttgccg aactgaatat cgcctgtgcc
    26281 aaagtactga ccgtaccgga actggaaagc aatccacagt atgtggctcg cgaatcaatc
    26341 actcagtggc aaacgatgga tggtcgcacc tgcaaagggc cgaatatcat gccgaaattc
    26401 aaaaataacc ccggacaaat ctggcgcgga atgccctcac atggcatgga cacggctgct
    26461 attttgaaaa atatcggcta cagcgaaaac gacattcagg agttggtcag caaaggtctg
    26521 gccaaagttg aggactaaac gcgtggcgtc agtcagcttg cctgactagc gcccgctcaa
    26581 taacaaacga acaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt
    26641 caaatgtggg acgatctggc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc
    26701 agcagcggag tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg
    26761 gcaaacctgt tttatgtgct ggggattcgc aaaggcgaca aggttgctct acatctcgac
    26821 aactgcccgg aatttatctt ttgctggttc gggttggcaa aaattggcgc gattatggtg
    26881 ccgattaacg cccgcctgtt gcgcgaagaa agcacgtgga tcctgcaaaa tagccaggcg
    26941 tgcctgctgg tgaccagtgc gcaattttat cctatgtatc aacagattca gcaggaagat
    27001 gcctctcagt tgcggcacat ttgcctgaca gatatggtac ttcccgctga tgatggcgtg
    27061 agttcgttta ctcaacggaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta
    27121 tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggg
    27181 gtggtgataa ctcattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca
    27241 ctgcgtgacg atgacgtcta cctgacggta atgcctgcgt ttcatatcga ttgccagtgt
    27301 actgcggcga tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc
    27361 gcccgcgcct tctggggaca ggtgcagaag taccgcgcca ccattaccga atgtattccg
    27421 atgatgattc gtacgttgat ggtgcagccg ccttcagcca acgatcggca acactgcctg
    27481 cgggaagtga tgttttatct caacctgtcg gagcaggaaa aagacgcgtt ttgtgaacgc
    27541 ttcagcgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc
    27601 gatcgccctg gcgataaacg gcgctggccg tcgattggtc gggcgggctt ttgctacgaa
    27661 gcggagatcc gcgacgatca caatcgtccc ctcccggctg gtgagctcgg tgaaatctgt
    27721 attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact
    27781 gcgaaagtgc tggaagccga tggctggcta cataccggcg ataccggata ccgcgacgaa
    27841 gagggctttt tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat
    27901 gtctcctgcg tggagctcga aaatatcatc gccacccatc caaaaattca ggatatcgtg
    27961 gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat
    28021 gaaggtgaaa cattgagcga agaggaattc ttctgcttct gtgaacagaa tatggcgaaa
    28081 tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa
    28141 ataattagaa agaatctgaa ataaatgaaa cggcagggaa ctaccctgtc agctaataat
    28201 cacgctctaa aacaatatgc attttttgca gggatgttgt cgtccctgaa aaagcaaaaa
    28261 tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt
    28321 acccttgatc gtccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa
    28381 gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga
    28441 gagaaattct tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat
    28501 gctgactttg gcccgggagg ttttgcggga ttaaccgaaa ttttcaatct cgacaaaccg
    28561 gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt tcgaactggc gctggcggca
    28621 gattttattg tttgtgccga taatgccagc ttcgccctgc cggaagccaa actgggcatc
    28681 gttcctgaca gcggcggcgt gctgcgtctg ccgaagatcc tgccgcctgc catcgtcaat
    28741 gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgcagcgttg ggggatagtc
    28801 aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc gcagcagctg
    28861 gttaacagcg ctccactggc gattgcggcg ctgaaagaga tctaccgcac caccagcgaa
    28921 atgccggtag aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccatcg
    28981 gttctgcatt cggaagatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg
    29041 gtgtggaaag gacgttaaca accgtgagct attacgcctt tgagggatta attccggtgg
    29101 ttcacccgac ggcgtttgtc catcccagtg cagtattgat tggcgatgtg attgtgggag
    29161 ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc
    29221 aagcgggagc caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca
    29281 tcgttgggga aaacggccat atcgggcacg gagcgatcct gcatggttgt gtgattggtc
    29341 gcgatgcact ggtcgggatg aacagcgtga ttatggatgg cgcgatcatt ggcgaagaga
    29401 gcattgttgc cgctatgagc tttgtcaaag cgggctttcg cggcgagaaa cgccagttgt
    29461 tgatgggtac gccagcccgc gctgtacgca gcgtgagtga cgacgagtta cactggaaac
    29521 ggttgaatac caaagagtat aaggatcttg tcgggcgctg tcatgcatca ttacatgaaa
    29581 cgcagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga
    29641 cgccgaaacg gtgatctttc cggatgcggc gtaaacgcct tatccgacct ggctggaaag
    29701 gaaccgtagg ccgtataaga tgcgccagca tcgcatccgg cgaccaggtc gcacggtatc
    29761 aacgaaacga cattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    29821 gttaagttcc gtgagcggaa cggcaggaat acgtggcgtc tttcttacgc cgactaattt
    29881 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    29941 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    30001 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    30061 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    30121 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    30181 ttcacaaatt aaaagtctgg ctatgactcg ttttgcagca attaaacaag gccatattaa
    30241 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    30301 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaacggtc
    30361 ctcatcagag aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccatg
    30421 acacgctatt atttgatctg cgcgtgcatt tcctgcaccg aaattacttt ttcagtcgca
    30481 tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc gtaatgcact
    30541 ttatattgca gcgcactgcg acggatcacg cgggagtctt caatcgcacg acgacctgag
    30601 gtggtgttga tgatgtaggt atattcgcca ttcttgatac ggtcctgaat gtgcggacga
    30661 ccttcatgca ccttgttcac cagacgcgga ttaatgcctg cttcgcccag cacaatcgcc
    30721 gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc
    30781 acgcgttctt tatcgccttc gcgcacggaa agcaacgcac gaccgtgttt cttcatggtg
    30841 gagttgctgc ccagctgcgc tttggcgaac gcttcagcga aggtgcggcc cacgcccatg
    30901 acttccccgg tagagcgcat ttctggccct aacagcgggt caacgcccgg gaatttattg
    30961 aacggcagca ccacttcttt caccgagtag tacggcggga taacttcttt ggttacgccc
    31021 tgctcagcca gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg
    31081 gtggctttgg agacgaacgg aacggtacgc gccgcacgcg ggttaacttc aatcaggtag
    31141 acttcgttgt ttttcaccgc aaactgcacg ttcatcaggc cgcgcacctg caattcgaag
    31201 gccagtttct gcacctgctg gcgcatcaca tcctgaattt cctgacttaa agtgtaggct
    31261 ggtagcgaac atgcagagtc accggagtgc acgcccgcct gctcgatgtg ctccatgatg
    31321 ccgccaatca gcaccatttc accgtcgcag atggcatcca cgtcaacttc tactgcgtca
    31381 tcaaggaagt ggtccagcaa cactggcgca tcgttagata cgctgaccgc tgtctggaag
    31441 tagcgacgca ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa
    31501 gacggacgca ccaccagcgg gtagccaatc tctttcgctt tctcaaccgc catttcgatg
    31561 gtggtaacgg tggcgttcgc cggttgtttc agtttcagac gctcaaccgc atgctggaag
    31621 cgttcacggt cttccgcacg gtcgatggca tccgggctgg taccgataac cggtacgcca
    31681 gcagcttcca gcgcgcgcgc cagtttcagc ggggtctgac cgccgtactg gacgataacg
    31741 cctttcggct tctcaatacg cacaatttcc agcacgtctt ccagagttac cggctcgaag
    31801 tagagacggt cagaggtgtc gtagtcggtg gagacggttt ccgggttaca gttaaccata
    31861 atggtttcat aaccatcttc gcgcagcgcc agcgaggcgt gtacacagca gtagtcgaat
    31921 tcgataccct gaccgatacg gttcgggccg ccgccgagca ccatgatttt ttcacggtcg
    31981 gtagacggat tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg
    32041 gcgaactctg ccgcacaggt atccacgcgc ttataaaccg ggtgcaagtc atactggtcg
    32101 cgcagcttgc ggatttctgc ttcacgcacg cccgcgagtt ttgccagacg cgcgtcggca
    32161 aagcctttgc gtttcagctg gcgcaggaag tcagcgttca ggccagtgat gcccacttct
    32221 gcgactttct cttccagacg caccagctct tcaatttgta ccaggaacca gcggtcaatg
    32281 ttggtcaggt tgaacacgcc gtctacggac aggcccgcgc ggaaagcatc ggcgatatac
    32341 cagatacgct ctgcgcctgc atctttcagt tcgcggcgaa ttttggtcag cgcttccgga
    32401 tcatcgaggc tgactttcgg gtcgaagcca gttgcgccca cttccaggcc gcgcagcgct
    32461 ttttgcaggg attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc
    32521 tgagtggtca gacggtcgtt cgccccggcg aatttctcga agttgaagcg aggaatttta
    32581 gtcaccacat agtcgatgga cggctcgaag gaggccggag tacgcccacc ggtgatgtcg
    32641 ttcatcagct cgtcgagggt gtaacccacc gccagtttcg ccgccacttt ggcaatcggg
    32701 aaaccagtgg ctttcgacgc cagcgcagag gagcgagaca cgcgcgggtt catttcgata
    32761 acaatcaggc gaccgttttt cgggttcacc gcaaactgga cgttggaacc accggtttca
    32821 acgccgattt cacgcagtac cgccatcgag gcgttacgca tgatttgata ttctttgtcg
    32881 gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg tgtggatgcc catcgcatca
    32941 aagttttcga tagagcagac aatgatgcag ttgtcgtttt tatcacgcac cacttccatc
    33001 tcgtactctt tccagccgat cagcgactca tcaatcagca gctctttagt cggagagaga
    33061 tccagaccac gggcgcaaat ttcttcaaac tcttcgcggt tataagcgat accgccgccg
    33121 ctaccgccca tggtaaagga tgggcgaata atgcacggga agccaacttc agcggcaacc
    33181 gccagcgctt cttccattga atgagcgata ccggaacgcg cggtttccag accgattttc
    33241 ttcatcgcta cgtcgaaacg gcggcggtct tcggctttat caatcgcatc ggcagtggca
    33301 ccaatcatgg tgacgccgaa ctcttccagc acgccctgac gctcaagctc aagcgcacag
    33361 ttcagcgccg tctgcccccc catggttggc agcaccgcgt ccggacgctc tttttcaata
    33421 atcttgcgta ccacttccca gtgaatcggt tcgatgtagg ttgcatcagc catttccggg
    33481 tcggtcatga tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcacgc
    33541 agggctttac acgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc
    33601 gggcccgcac ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta
    33661 ctcctgatta cttagcggtt ttacggtact gctcaattaa ctcgataaag tggtcaaaca
    33721 acggcgcggc gtcgtgtgga ccagggctgg cttccgggtg tccctggaag ctgaacgccg
    33781 gtttatcggt acgatgaatg ccctgtaacg taccgtcgaa cagggattta tgtgtgacac
    33841 gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtaatca
    33901 tcaccacatt tttctcaaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca
    33961 tttttacagt cttcgcaccg ctcgccagcg ccagcagctg atgaccgagg cagatgccga
    34021 ataccggaat atcggtttcg aggaatttct ggatggcggt aatggcgtaa tcgcacgggg
    34081 ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacgtctt
    34141 ccgcagaagt ttgcgccgga acgatggtca gacgacagcc tctatccacc aacatgcgca
    34201 ggatgttgcg cttggcacca aaatcataag ccacaacatg gaacggcagc tcgtcttctt
    34261 tcttcgcttc tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt
    34321 ctgcggtggt cacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt
    34381 tttctaatgc cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc
    34441 ctttctcgcg cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt
    34501 tatggcgctt caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg
    34561 gcaggtcgcg aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat
    34621 tagtgccgac attaccaata tggggataag taagagtaac gatttgacga gaataggaag
    34681 gatcagtgag gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg
    34741 ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata
    34801 gcgctgactt aatcaaaaca ccctccagag aatattcact cactttattt gcatattaat
    34861 tcacaatgat gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg
    34921 gcattctgga gatatgggcg ctaaaagtca acttaatggc gggatttttt atattatttt
    34981 gtggctcatg catgttttac aggcattaag cgacaaaaag atcatataac atgttctgga
    35041 aaacgcttgc gcagcaagaa gattgcatga aatcaaaatg agcagaaaaa agtggaccaa
    35101 atggtcaaaa tttacattaa aacaacaaaa acagaataca aaacatgtaa ttttcagcac
    35161 ttgcattaaa ttt
//
LOCUS       DAZGTJ010000047        35135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000047 DAZGTJ010000000
VERSION     DAZGTJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.047"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            252..1907
                     /gene="aslA"
                     /locus_tag="ACHK8E_002804"
     CDS             252..1907
                     /gene="aslA"
                     /locus_tag="ACHK8E_002804"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP0345048.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2065..3300)
                     /gene="aslB"
                     /locus_tag="ACHK8E_002805"
     CDS             complement(2065..3300)
                     /gene="aslB"
                     /locus_tag="ACHK8E_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0345049.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3447..3523)
                     /locus_tag="ACHK8E_002806"
     tRNA            complement(3447..3523)
                     /locus_tag="ACHK8E_002806"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3487..3489),aa:Pro,seq:tgg)
     gene            complement(3566..3652)
                     /locus_tag="ACHK8E_002807"
     tRNA            complement(3566..3652)
                     /locus_tag="ACHK8E_002807"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3616..3618),aa:Leu,seq:cag)
     gene            complement(3673..3748)
                     /locus_tag="ACHK8E_002808"
     tRNA            complement(3673..3748)
                     /locus_tag="ACHK8E_002808"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3713..3715),aa:His,seq:gtg)
     gene            complement(3807..3883)
                     /locus_tag="ACHK8E_002809"
     tRNA            complement(3807..3883)
                     /locus_tag="ACHK8E_002809"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3847..3849),aa:Arg,seq:ccg)
     gene            complement(3986..5371)
                     /gene="yifK"
                     /locus_tag="ACHK8E_002810"
     CDS             complement(3986..5371)
                     /gene="yifK"
                     /locus_tag="ACHK8E_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0345050.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5562..6302)
                     /gene="wecG"
                     /locus_tag="ACHK8E_002811"
     CDS             complement(5562..6302)
                     /gene="wecG"
                     /locus_tag="ACHK8E_002811"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP0345051.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6305..7657)
                     /gene="wzyE"
                     /locus_tag="ACHK8E_002812"
     CDS             complement(6305..7657)
                     /gene="wzyE"
                     /locus_tag="ACHK8E_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP0345052.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7654..8733)
                     /gene="rffT"
                     /locus_tag="ACHK8E_002813"
                     /gene_synonym="wecF"
     CDS             complement(7654..8733)
                     /gene="rffT"
                     /locus_tag="ACHK8E_002813"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP0345053.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8730..9980)
                     /gene="wzxE"
                     /locus_tag="ACHK8E_002814"
     CDS             complement(8730..9980)
                     /gene="wzxE"
                     /locus_tag="ACHK8E_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP0345054.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9982..11112)
                     /gene="rffA"
                     /locus_tag="ACHK8E_002815"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9982..11112)
                     /gene="rffA"
                     /locus_tag="ACHK8E_002815"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP0345055.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11117..11791)
                     /gene="rffC"
                     /locus_tag="ACHK8E_002816"
                     /gene_synonym="wecD"
     CDS             complement(11117..11791)
                     /gene="rffC"
                     /locus_tag="ACHK8E_002816"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP0345056.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11769..12650)
                     /gene="rfbA"
                     /locus_tag="ACHK8E_002817"
     CDS             complement(11769..12650)
                     /gene="rfbA"
                     /locus_tag="ACHK8E_002817"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0345057.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12669..13736)
                     /gene="rffG"
                     /locus_tag="ACHK8E_002818"
     CDS             complement(12669..13736)
                     /gene="rffG"
                     /locus_tag="ACHK8E_002818"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0345058.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13736..14998)
                     /gene="wecC"
                     /locus_tag="ACHK8E_002819"
     CDS             complement(13736..14998)
                     /gene="wecC"
                     /locus_tag="ACHK8E_002819"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP0345059.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14995..16125)
                     /gene="wecB"
                     /locus_tag="ACHK8E_002820"
     CDS             complement(14995..16125)
                     /gene="wecB"
                     /locus_tag="ACHK8E_002820"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP0345060.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16181..17227)
                     /gene="wzzE"
                     /locus_tag="ACHK8E_002821"
     CDS             complement(16181..17227)
                     /gene="wzzE"
                     /locus_tag="ACHK8E_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP0345061.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17239..18342)
                     /gene="wecA"
                     /locus_tag="ACHK8E_002822"
     CDS             complement(17239..18342)
                     /gene="wecA"
                     /locus_tag="ACHK8E_002822"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP0345062.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18582..19841)
                     /gene="rho"
                     /locus_tag="ACHK8E_002823"
     CDS             complement(18582..19841)
                     /gene="rho"
                     /locus_tag="ACHK8E_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP0345063.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19926..20027)
                     /gene="rhoL"
                     /locus_tag="ACHK8E_002824"
     CDS             complement(19926..20027)
                     /gene="rhoL"
                     /locus_tag="ACHK8E_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP0345064.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20168..20497)
                     /gene="trxA"
                     /locus_tag="ACHK8E_002825"
     CDS             complement(20168..20497)
                     /gene="trxA"
                     /locus_tag="ACHK8E_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP0345065.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20628..21893
                     /gene="rhlB"
                     /locus_tag="ACHK8E_002826"
     CDS             20628..21893
                     /gene="rhlB"
                     /locus_tag="ACHK8E_002826"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP0345066.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            22027..23511
                     /gene="gppA"
                     /locus_tag="ACHK8E_002827"
     CDS             22027..23511
                     /gene="gppA"
                     /locus_tag="ACHK8E_002827"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP0345067.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23558..25579)
                     /gene="rep"
                     /locus_tag="ACHK8E_002828"
     CDS             complement(23558..25579)
                     /gene="rep"
                     /locus_tag="ACHK8E_002828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP0345068.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25666..25947
                     /gene="ppiC"
                     /locus_tag="ACHK8E_002829"
     CDS             25666..25947
                     /gene="ppiC"
                     /locus_tag="ACHK8E_002829"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP0345069.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25993..27468)
                     /gene="ilvC"
                     /locus_tag="ACHK8E_002830"
     CDS             complement(25993..27468)
                     /gene="ilvC"
                     /locus_tag="ACHK8E_002830"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP0345070.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27618..28508
                     /gene="ilvY"
                     /locus_tag="ACHK8E_002831"
     CDS             27618..28508
                     /gene="ilvY"
                     /locus_tag="ACHK8E_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP0345071.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28505..30049)
                     /gene="ilvA"
                     /locus_tag="ACHK8E_002832"
     CDS             complement(28505..30049)
                     /gene="ilvA"
                     /locus_tag="ACHK8E_002832"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP0345072.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30052..31902)
                     /gene="ilvD"
                     /locus_tag="ACHK8E_002833"
     CDS             complement(30052..31902)
                     /gene="ilvD"
                     /locus_tag="ACHK8E_002833"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP0345073.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31967..32896)
                     /gene="ilvE"
                     /locus_tag="ACHK8E_002834"
     CDS             complement(31967..32896)
                     /gene="ilvE"
                     /locus_tag="ACHK8E_002834"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP0345074.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32916..33179)
                     /gene="ilvM"
                     /locus_tag="ACHK8E_002835"
     CDS             complement(32916..33179)
                     /gene="ilvM"
                     /locus_tag="ACHK8E_002835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP0345075.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33176..34822)
                     /gene="ilvG"
                     /locus_tag="ACHK8E_002836"
     CDS             complement(33176..34822)
                     /gene="ilvG"
                     /locus_tag="ACHK8E_002836"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP0345076.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34825..34875)
                     /gene="ilvX"
                     /locus_tag="ACHK8E_002837"
     CDS             complement(34825..34875)
                     /gene="ilvX"
                     /locus_tag="ACHK8E_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP0345077.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34962..35060)
                     /gene="ilvL"
                     /locus_tag="ACHK8E_002838"
     CDS             complement(34962..35060)
                     /gene="ilvL"
                     /locus_tag="ACHK8E_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP0345078.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8534 a         9692 c         8886 g         8023 t
ORIGIN      
        1 tatattaatt gccgttaaaa ctaaaaacag catcaataat caacgcgata taacatcacc
       61 gccttacata tcacctgcgc cagaggtagg attgaaaacg ctctcctgat tttccaattc
      121 attttctaga taaataaata atttattttt atcactatta tttatgcaat catcctgtca
      181 gggagaggga tctcaattat caatgcttaa ttacgtcatc attttgacga catgaaaccg
      241 gagcattttg catggaattt tcgttttcac ccaaacttct tgttgtggct gtcgccgccg
      301 ctcttccact catggccaac gcagcagata ccccgtcaac cgccaccgca cgcaaaggct
      361 ttgccggata cgatcaccca aaccagtatc tggttaaacc ggcgaccact attgccgaca
      421 acatgatgcc ggtgatgcag catcctgcac aagacaaaga aacccagcag aagctggcag
      481 aacttgagaa aaaaaccggt aagaaaccga acgtggttgt tttcttgctg gatgatgtgg
      541 gctggatgga tgttggcttt aacggcggcg gcgtggcggt gggtaaccct acaccagata
      601 tcgacgccgt tgccagccag gggctgattt taacttcggc gtattctcaa ccgagttctt
      661 ccccaacccg cgccacgatt cttaccggac aatactccat ccaccacggc attctgatgc
      721 cgccaatgta cgggcaaccg ggcggtctgc aagggttaac cacgctgccg cagttgctgc
      781 acgatcaggg ctacgtcact caggccatcg ggaaatggca tatgggggaa aacaaagagt
      841 cgcagccgca gaacgttggc tttgatgatt tccgcggctt taactcggta tccgatatgt
      901 ataccgaatg gcgtgatgtt cacgtcaacc cggaagtcgc cctgagcccg gatcgttctg
      961 aatacatcca aaaactgccg ttcagcaaag atgacgttca tgccgtgcgc ggcggtgaac
     1021 aggaagccat tgccgacatt acgccgaaat atatggaaga cctggatcaa cgctggatgg
     1081 aatatggcgt taagttcctc gacaagatgg cgaagagcga taagcctttc ttcctctact
     1141 acggcactcg cggctgtcac ttcgataact acccaaatgc gaaatatgcg ggtagctctc
     1201 cggcgcgcac ctcttacggc gactgcatgg tggagatgaa cgatatcttc gccaatctgt
     1261 ataaagcact ggagaaaaac ggtcagcttg ataacacgct gattgtgttc acctctgata
     1321 acggtccgga agccgaagtt ccgccgcacg gtcgcacacc gttccgcggt gcgaaaggtt
     1381 ctacatggga aggcggcgtt cgtgtgccaa ccttcgttta ctggaaaggc atgatccaac
     1441 cgcgtaaatc tgacggcatt gtcgatctgg cggatctctt ccctacggcg ctggatctgg
     1501 cggggcatcc tggggcgaaa gtagcgaatt tagtaccgaa aaccaccttt atcgatggtg
     1561 tggaccagac atctttcttc ctgggaacaa atggtcagtc taaccgtaag gccgagcact
     1621 acttcctcaa cggtaaactt tctgctgtgc gtatggatga gttcaagtat cacgtcctga
     1681 ttcagcaacc ttacgcttat acccagagcg gatatcaggg tggattcacc ggcacagtaa
     1741 tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga tccgcaggaa agcgactcta
     1801 tcggcgtgcg ccatattccg atgggtgtac cgctacagac cgaaatgcac gcgtatatgg
     1861 agatcctgaa aaaatatcca ccacgcgcgc agattaaatc tgactaagcc ggcgctatcg
     1921 ctgagatctg cctttgccgg atgcgatgct gacgcatctt atccagccta cagaacgctg
     1981 caatttattg aatttgcaat gattgtaggc cggataaggc gttcacgccg catccggcaa
     2041 tcaaccgcag gcggccgccg atttctactt attcaccacc atcaaatgcg cctgcataat
     2101 gtcgctggcc ggacgaccgt gcgccagcaa atcagccatt gctttaagat atggcggtag
     2161 atggcggaaa taacgctgat acccggcaca caaataattc agcccgggtt tgccgctggc
     2221 atcgagcata aagcggtgtt tcgggcagcc tccccagcac gcttttaaca cgttgcaact
     2281 gcgacactgt gccggtaact gcttgaattt atcttcacca aatgcctgct gttgcgggga
     2341 atcaatcatt tctgcaatcg tctgctgaag catattccct agtcgatact gcggataaac
     2401 atagtgatcg caggcgtaaa catcgccgtt gtgttcaaca atcaccgagc gcccacaggt
     2461 tggctgatga tggcaaaccg cgcccggtgc gccgacaaaa ttggcgaacg cccattcgat
     2521 attcattacg aaaaccttgc cgacgtcgcg cttaatccag tggtcgaaaa tcgccaccag
     2581 aaactcaccg aactcttgcg ggcacaccga ccattccgtc agttcgccct gaatatcgcc
     2641 gggagcatgt agcttaagtc cagcatgagc agccgtttca tcagccaggc gctcgaccac
     2701 cggaataaac tggatgaact caacacctgc atcacacaga aaatcataga cttgcaaagg
     2761 ttgtaacgca ctggtgcgat ttacgcagac cagcacgtta tagtcgacat gatgtttttg
     2821 caggagcgtc agggcacgca tcaccagctt atgcgttggt ctgccgcctt tggtcacgcg
     2881 atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa aatgattttc
     2941 tgccagaaat gcacaccatt cgtcatcaag cagcacgccg ttagtctgga agctgttact
     3001 tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt aaaaatccag
     3061 cccgagtaac gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt gggtttcgct
     3121 ggcagcgata tagtgacgga catacgcttc cagcgtgtca tcgtccatat gcgtgactgg
     3181 cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt tcagattaca
     3241 atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct gttgcagcat
     3301 ggtcgctcct tgcctgaagt catccttcaa taagttaagg gcgaaatcat accgtggcgc
     3361 aatgcgttat tacgggcgtt tattgcgaag ggaaagtgac gaaatgcaca gaaaacaaaa
     3421 aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac ctccgaccca
     3481 ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact gctttttgaa
     3541 tttttagttc aattctttaa agtcgtggtg cgatgggggg gacttgaacc cccacgtccg
     3601 taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcattgtcac
     3661 aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga atcacaatcc
     3721 agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg tattactacc
     3781 accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac ctgagacctc
     3841 tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc cgttgcgggg
     3901 gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa ttgcgcgttt
     3961 ggttacggtt tgtgcgcttt gctgcttatt tctccagttt atgcgcttta ccgtggcgat
     4021 taagcccaaa aactttataa atcgccgtca ccgtcagcat aaagatgatg ccgacaaaca
     4081 gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca
     4141 ttgttacgta gttggcccac gggaacagga ttgagcggaa cggatggctg gcaattgccg
     4201 ctttatgcgc acgacgaaaa cgcagctggc taatcaatat cacaaaccat ggcaccatcc
     4261 ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt
     4321 agttcaggca tgagccaatt aacaggatag caatagatac tgccacaccc gcaaccggta
     4381 cgccgtgacg ggaaactttc gccatcgccg tcggtaactg acggtttttc gccaatgcgt
     4441 agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca
     4501 ccacaaagtt gataatgccc gccgctgcgg taatgccgat tttggcgaaa gtcagtacga
     4561 acgggctgcc gttactgcct atttcattcc acgggaagat ggtgacgata acgaaaatcg
     4621 cgcctacgta gaaaatcagg atacgccaca acactttgcc tacggcactg cgcagcgtca
     4681 cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg
     4741 ccaccacaat acacaatgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat
     4801 gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaaacca aagaaaatca
     4861 cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga
     4921 actcgatttc gccgtacaac cgcactgccg ccagattcgc cagcgccacc agcgccaccg
     4981 cgatcaatgc gggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa
     5041 tggcggtgat ttctgagatc cccaccgcca tccacataaa ccagtaagac caggcggtga
     5101 gatagccaaa aaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaaccg
     5161 gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaagacgaac agcccggcga
     5221 tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg gcccccataa
     5281 acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc
     5341 cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc
     5401 cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg
     5461 agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg
     5521 cgacggagtg accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtag
     5581 tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc
     5641 aggcggtaga gccactccag ccccagcgtt tgccagattt tcggtgcgcg ttttacgtga
     5701 ccggtgaaaa catcgtaggt cccgccaacg cccatataca gcgcatctgg atgcaccaga
     5761 cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc
     5821 gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca
     5881 tcctgactgc caacgatatt cacattccac tggttgcgca gtttagcttc agtttgcgcc
     5941 agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc tgcgcgcgcc
     6001 atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt
     6061 ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt
     6121 agctccctga cctcagcatt atcttcaatg gtcagcattt tttcggcatt aatggcaacc
     6181 agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcgtg ctgcatatca
     6241 cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc
     6301 attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg
     6361 cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg aagaccacga
     6421 taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc acgatcatat
     6481 tgaagatcgc cccgaagcag aaactgtgca atatcgcagc cttatagcga ttagtctcgc
     6541 ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagaccaacc acaatcgccc
     6601 cgagcgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc gtaggcgaga
     6661 tcgccagccc ggagtggtta ttcagcactt cccaggtaaa gtagttggcg gagttcagta
     6721 ccatactcgg gcgacccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa
     6781 tgggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct
     6841 cccacggcga aaaggtatcg cgggtgagat agagaaacgt atagaacgct tcatcgccgc
     6901 tcacattcat tccataacgt tttagcgcca gccagaacat gcccacaatc cccagcacgc
     6961 ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga
     7021 tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatgtaa gtcaacaagc
     7081 caaaggcaac cgtgctgacg aggaaaaaca gccacgcttt gctatcctgg cgcagaaaat
     7141 aaaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaatgcc acgccggaga
     7201 cttcgctgga aaagatctga ctgtaagaat taagccggaa cagcaaaaag ccgttgtgca
     7261 tgaagaagat gccgacgctc accagagcga tgcccatcag gatcacccac gtgagattgg
     7321 tctccacgcg gttcatggta aacagcggac ggcgcggtgc atcagcaacg cgtttgcgta
     7381 gacgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcctgcagaa agcaacgcct
     7441 gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc acgctggtca
     7501 gcggaaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca ttgaagttaa
     7561 agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacaacgtg cagagcagcc
     7621 agacaacaaa caggccactg aattgcagca gactcatgcg acctccccgg cggcaatcgc
     7681 caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc
     7741 caccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat catcggtagt
     7801 aaacagtact ggcaaatgct gttccgtcat atcctgccag aacggatttt cccggttaag
     7861 cacacaagga atgcccgcct gaatcagtaa gcaaagcgta ccaatgccct gctggcgggc
     7921 aaaaataaag taaccgagat cgcactgacg aagtagcgtc agataggcgt caaattccag
     7981 tttttcgctc agaacttgca gattttcttc gctgaataac tccagtcccg cctgacgaac
     8041 ttcctcgatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca cttttaccgt
     8101 atcgccaaat tgctgatgaa cggcgcgcaa ggcagcaata tgctcattgc tgcggtcacc
     8161 ggagttcccc accagaatgg tcatttttac ttcacgttgc cgatcgttca ccatcgtatt
     8221 aagagaagag tccatccgcg tcgggaagta cagcagttcg ccccgcacct ttgggtgcgt
     8281 tttggcaaaa aagctcaaat caccgcgggt ggcaaataca cagccgactc gcttttgcgc
     8341 caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagtt cgtacaggtc
     8401 tgccccccag atatgccagt aaaactggct gggcttaatg ccaccactca gcagagccag
     8461 ccacagtgtg ggattgaact gaccgtggaa gaaaaaacgc tgctgacggt tagcttttgc
     8521 tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc
     8581 cagacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc
     8641 gctcgtcgcg gccagcgcgt cgttgaaaaa ccgcagaacg gttcggttat ggtgagggat
     8701 atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg
     8761 ccacaacaaa gagaaaaata gacgatatag gttgccatat aggcctgcgc tgcgcccagt
     8821 gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa ctggctgact
     8881 tccgccagaa tataaaaccg cagtgacgct ttggcgatca ccagataacc aaagacataa
     8941 gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg catagcggta
     9001 aatttattcg acaacagcag ccagatagca aaatcacgca gcaaccagac ggtaaaactc
     9061 gccgccgcca ctgccggtaa gacgaatttt agcgatttaa ccacttcccg ggtgatatcg
     9121 cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata
     9181 aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac ctcatcccag
     9241 ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac
     9301 gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg
     9361 ggtttcagat agcttaacgg gatgacacca cgtttgatca acatgatggc ggcaggaatt
     9421 accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc gcccaaacgg
     9481 taactgacgt agtacgcaag aacgccaatc aagctgccaa caatcagcga taacgcatta
     9541 cctgcggcat cgcgaaagcc tttcatcaac gccagtaaca ggttgcccca ggcgatcccc
     9601 atttgcacca acgccactaa acgcaccagc tcctgatagt cggtattacc aaacaatccc
     9661 tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaaaccaagt
     9721 accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt ggggattatc atggtactgg
     9781 gcaacgtatt tggttacgcc gttaaagatg cctgccccgg caagcacgcc gagcacggta
     9841 atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga cactgccagc
     9901 aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc agtccacaag
     9961 gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaggtcgcaa ttaccgtacg
    10021 ctgattgacg ggcgacaggt tgtagaacaa cggcaggcgc agcaggcgct cgctctcttt
    10081 ggtggtgtag cgatcttcac cgtggaactc accaaagcgt tcccccgcag ggcaaccgtg
    10141 cagcggaatg taatggaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc
    10201 gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct gcacgcagcc
    10261 atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggtgcta acgcatcgta
    10321 gtagttttgc cacagcgcca gacgttgctg attgatacga tccgctgctt ccagttgcgc
    10381 ccacaggtac gcagcttgca aatcggacat caaatagctg gagccgatat cgcgccaggt
    10441 atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccttttt cacggatgat
    10501 ctcggctcgt tcgatcaacg ctttatcgtt aatcagcgtc gcaccgcctt caccgcccgc
    10561 cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc
    10621 acgccctttg taagtggaca taacgccctg agcggcatct tccaccacaa acagattata
    10681 ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt aatggaccgg
    10741 cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc agcgtttcgt cgatgttcat
    10801 agtgtccggg cgaacatcca caaagacgat tttcgcgcca cgcagcacaa aggcattggc
    10861 ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag
    10921 cagcgccgcc atctccagag aagcggtgca ggacggcgtc aacagcactt tggcgctgcc
    10981 aaaacgttgt tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt
    11041 gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg gcggtgcgtt
    11101 aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac
    11161 tttgtatgta tcgtttaagc gcggcggtgt tgcccatctg ggtcgccacc cgcaaagttg
    11221 ttttaccgcg agcatacgcc cagtttagcg ctgtttgcat cagctcagca cctgcaccgc
    11281 gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa gagacatagc
    11341 cgcgaatatc gccggacgcc gaacgtaaaa tgaggcattg atgatcaaag gtgccgcgca
    11401 cggcattttc aatccactgt gcataaaagc gaccgctggc gtcaggcgca taccacggcg
    11461 cacgaaaacg gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg
    11521 tctcttgtgc cactacagca ccactatcac tgacattgtt tacgggtagc gccaaatcaa
    11581 cttcaccttc taccagggag aatcccagct gttgcaaggc atccagttca tccgtatttg
    11641 atgccgcaat tttggcctgc acccgtgacc acggcgctaa ggcgtctggc gtcaggagcg
    11701 gtgcttcagg actaatgcgc acaatggcgc tgttaacacc aaagaaggcg ttttcccagg
    11761 ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag
    11821 ccagttttcg ccagcgagct ggcggcgcgc ttcacgcctt cgtcatcaag ccagccgtta
    11881 cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc caccgtctgc
    11941 acaaaggtgc tggcttcaat caggctgtcg tgagtgccag tgtccagcca ggcaaatccg
    12001 cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta
    12061 atctccagtt caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg
    12121 tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg cttttcttcc
    12181 agcgagatag cgcggaaatt gtcgtcaaac tccacgacgc caaagcgttc cggatccatc
    12241 acctgatagc caaataccgt cgcgccttgc gtgcgcgccg caacatgacg cagcttcgga
    12301 ctgaagccct gtccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccgttg
    12361 aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccggactggg ctgttcggca
    12421 tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta
    12481 tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag caccgacagc
    12541 ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta
    12601 atcggatgca atcgggtgcc ggaaccgccc gccaggataa tacctttcat gtttgcctcc
    12661 gctgaaaatt agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc
    12721 ttccaccagc tttcattagc cagataccac tgaaccgttt tacgcattcc actttcaaag
    12781 gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat ggcataacgc
    12841 agatcatgcc ccggacggtc agcgacaaag gtgatcaggt cacgatagtg cgccacgccg
    12901 tgcggcttgt tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga
    12961 ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc
    13021 acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat ttgctgcccg
    13081 ttgccatata ccggcagcgg tttacccgcc agcgcgttga ggatcatcag cgggatcagt
    13141 ttttccggaa agtggtaagg gccgtagtta ttcgagcagt tggtgataag cgtcggcaga
    13201 ccataggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga cacggaataa
    13261 gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca
    13321 ccatatactt cgtcggtaga gatatgatga aaacggaacg ctgatttttt atcttccgtc
    13381 agcgcattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt
    13441 tcaacaaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc
    13501 atgacacagt ctggctgatg ctcagtgaat acgcgcgcca gttctgcccg atcgcagata
    13561 tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga catcagattt
    13621 ccggcgtagg tcagcttatc gaccactacc accgcgtcgc tcgtttcgtt gatgatataa
    13681 cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt tctcatcagc
    13741 gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca ttgataactt
    13801 tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa
    13861 gctgcgccag tgtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa
    13921 caaccagagt ttcgccgcta tgccactggg cgatcagttc agcgatttcc atcgctgggc
    13981 tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt
    14041 cactggcgcg tttatcggta gcagcaaggc agtcggcaac cgccgctttc acctgatcga
    14101 taacccagaa cggtttgtga tcgttcactt cgcgcgcggt acggataagc cgcgcctgct
    14161 gcgggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag
    14221 gaccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga
    14281 cgttaattcc ctgatcggca caaatcagcg acagttcgtt agcaaaagcg atattcacat
    14341 cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac
    14401 actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg ggccgaacaa accggcgtca
    14461 taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc ggtaacacgc
    14521 gttccgggca gtaagcaatg ttgacgtccg ccaactcgcc cacctgctgt gggaaagtga
    14581 gatctggacg catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg
    14641 tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg
    14701 attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt acagcaatca
    14761 gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag
    14821 tttttactac gctcgccaaa tcaggttcga cgatatggat ttcgccacga ttgatggtat
    14881 caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag gcaaacgcag
    14941 ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca aaactcatag
    15001 tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata
    15061 cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa gacgcgtcac
    15121 ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc
    15181 ttccggacgc tcagtggtat cgcgcattac cagcaccggt ttccccagcg aaggcgcttc
    15241 ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa
    15301 cggtaaatac tcctggggat cgatcagaat gacatttttc acgtgcccca gaatgcgatt
    15361 gaccggctct ctgacgttcg ggttgagatg cactggatag acaatctgga tgtcctggtg
    15421 cgtggtggcg atgtctgcca gcgcatggca gatttcttca aagccacgac cgaagctctc
    15481 acgcctgtga ccggtcacca gaatcatttt tttattgggg tcgataaacg ggtaatttgc
    15541 cgccagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc ataacagtgc
    15601 atcaatgact gtattaccag taatgaagat tcggctatcc gcaacgtttt cacgcagcaa
    15661 gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc cggtcaatgt
    15721 acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgcagac cagcctcaac
    15781 gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgtcgtagt
    15841 atcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc
    15901 cagaatccga caggttatct ctgtaaggcc ctgtcctggc tgcattatgt tgagatcgta
    15961 gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc atctcccgat gctgcgcagt
    16021 gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggcgccat
    16081 cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga
    16141 ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag caacggcggg
    16201 ttaatgcgac accagccccg atcagtcccc cgacaatgcc ccacataatc atcaggaagg
    16261 cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgaaaatag cgataggtct
    16321 gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggcc cgattctgat
    16381 catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca
    16441 ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg
    16501 cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgttcaata ctgttcatcc
    16561 ggcgatcgta gatggctttc gccacctctt cctgacgctt cacctgagct ttcatctgga
    16621 tggtgcgtgc cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc
    16681 tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcagtt tcggcaatca
    16741 gcttcacgct gtcattgacc gcgcgggtga agtcaccggg aataaacacg atgttgttga
    16801 tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgtttgtaat
    16861 agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact
    16921 ctttataagc ttcgtccatg accgatggtt ggtcggcaga agccatgttt gaacggacat
    16981 ccaggttacg caaaaattgc tgctgtgagt aatatccccc cagcatattc accgttggac
    17041 gatcggtaat cgctgtcgag ctccactcct ggcgagcaaa aaaagtatac gccagcgcga
    17101 ttaacgcaaa cgccagcccc atgccaataa tccacagctt cccagcccac aaggtacgaa
    17161 acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt
    17221 gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag
    17281 tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttga tgcaatatcc
    17341 atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga caaaatgaga
    17401 atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa tcagcacaaa
    17461 cgcctggcgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtctgg
    17521 agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag
    17581 cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgagttt tgccctgggt
    17641 cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg catcccccat
    17701 aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg gcaggatggc
    17761 ggcgatcatc gcaaagcacc agattgcgag actggtttgc ccgtcgaacc acaaaatcat
    17821 accgattgct gcaaacgaga cgcaggacaa cccacccagc aagccatcaa tgccatcaac
    17881 catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg
    17941 tccgagcacc atctcccagg agccaaagat ataacctagg ctactgagat aaagtttgcc
    18001 gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt ttacgctgat
    18061 atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca ccagcacaag cgagatagag
    18121 agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac
    18181 cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt taggtttatc
    18241 cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg tcgtgaataa
    18301 aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg ctctcagaga
    18361 aaattattag cagaagtata accacgaaga cctttattca gaagggaaag tccgttccga
    18421 acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa cacaccagca
    18481 aaatccagga attttcctaa cattgccagc gcggcattaa gattacagat ataaaagcaa
    18541 aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt
    18601 tcgaagaaat cgtcattggt cttggtcatt gccagtttat taatgaggaa ttccattgca
    18661 tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc
    18721 tgagtagtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat tgccgggaag
    18781 acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac
    18841 tcttcgtaga taacttcgtc catcttggaa ccggtatcga taagcgcggt agcgatgatg
    18901 gtcaggctgc cgccctcttc cacgttacgc gccgcaccga agaagcgttt cggacgatgc
    18961 agggcgttgg catccacacc accggtcagc actttacctg acgccggaac aacggtgttg
    19021 taagcgcgcg ccagacgagt gatggagtcg agcaggatga taacgtcttt cttgtgctca
    19081 acaaggcgtt tcgccttctc gatcaccatt tccgcgacct gaacgtggcg agatgctggt
    19141 tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc ggttacttct
    19201 tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg
    19261 ctctgagcaa tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga
    19321 ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcggt taaatcttca
    19381 gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg ggttaagttc
    19441 tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc
    19501 agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca
    19561 ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg
    19621 gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt
    19681 acaccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat ggcaaaaata
    19741 atgtcctgct tacgcatacg agccaggttt tccagcccca tattttcgcc gagagtgatc
    19801 agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa
    19861 cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt
    19921 tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa
    19981 cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg
    20041 ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca
    20101 ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga
    20161 aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc
    20221 ccactttggt tgccgccact tcaccgtttt tgaacagcag caaagtcggg ataccacgga
    20281 tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt
    20341 tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc
    20401 accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa
    20461 aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc
    20521 tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac
    20581 cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac
    20641 atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg
    20701 aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg
    20761 cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta
    20821 cgtcaacgtt tcattatctt ctctctcatc ccgcgattgc cgatcgcaag gtgaatcaac
    20881 cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccac gccgacgcgg
    20941 agccacttgc gcaagctacc ggcctgaagc tgggtctggc ttacggtggt gatggctacg
    21001 acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc acggggcgtt
    21061 taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta gtggtactgg
    21121 acgaagccga tcgtatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc
    21181 gtatgccgcc tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt tcgtaccggg
    21241 tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg gaaccggaac
    21301 agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc
    21361 gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaaca
    21421 ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt
    21481 tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg
    21541 gtgatttgga tattctggtt gccaccgatg ttgccgcgcg tggcctgcat atcccggctg
    21601 tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg
    21661 gtcgtacagg tcgcgctggc gcaagtggtc attccatcag cctggcatgt gaagagtatg
    21721 cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta agcaaataca
    21781 atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccgcgtacag
    21841 gcaatggtcc gcgtcgtact ggtgctccgc gtaatcgtcg tcgttcaggt taaataaaat
    21901 gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc ccgatgcgca
    21961 tgctgtagta agtgatttgg gtgaaagaac gcagccaaca cagagacaga ttgaaggatg
    22021 aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc gaatagtttt
    22081 catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aattaaacgc
    22141 aaagtgcgtc tggctgctgg cctgaatagc gaaaatgccc tgtctaatga agcaatggag
    22201 cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc tccctcgcaa
    22261 attcgcgttg tcgctacggc gacattacga ctagccgtca atgcgggtga ttttatcgcc
    22321 aaagcacagg aaatccttgg ctgcccggta caagtcatca gcggtgaaga agaagcccgc
    22381 ctgatttatc agggggtggc ccacacaaca ggcggtgccg atcagcgcct ggtggtggat
    22441 ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac ctcgttgttc
    22501 agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg taatctggcg
    22561 caggaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgcagat
    22621 gaattacggt atcacggctg gaaagtgtgc gttggcgctt ccggcaccgt gcaggcgtta
    22681 caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa
    22741 ctgaaacaac gcgccattca ttgcggtcgg ctggaagagc tagagattga cgggctgacg
    22801 ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatagccat ttttaccgaa
    22861 ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggt
    22921 atgttgcatc ttaccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcagcgc
    22981 cgctttatga tcgatattga tcaggcacag cgcgtagcca aagttgcggc taacttctac
    23041 gatcaggtgg aaaatgaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc
    23101 tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagcgccgca acacgctgct
    23161 tatctggtac gtaatctgga tcttcccggt tttacccccg cacagaaaaa actgctggcg
    23221 acgctactgc tcaaccagac taatccggtc gatctctcat cgctgcatca gcaaaatgcc
    23281 gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc gcctggctat catttttgcc
    23341 agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg
    23401 accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga gattattgat
    23461 caggaaagcc agtggcagag ctatgtccac tggccgctgg aagtgcatta atgagtaagt
    23521 gccggatgcg atgctgacac atcttttccg gccttgatta tttccctcgt tttgccgcca
    23581 tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga
    23641 ccactttgcg ctcctgttcc caaatcaaat catcctgcgg cagctccagc aggaagcggc
    23701 tcggctccgg gcgcaccagt tctccgtact gacggcgttc tttacacagc gtaaaggtca
    23761 attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca tcgatattat
    23821 cttcatcgat gctgctctgg tgcggcaaaa acccctcttc cataccgacc atgtagacat
    23881 aaggaaactc cagccctttc gacgcgtgga gagtcatcag ttgcacctga tccagctctt
    23941 cttcactctc accacgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga
    24001 gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt
    24061 ggttgacgtt cttcatgcgc atttcggcgg ctttcgggct gggcgatgtt tcgtacagcc
    24121 aggattcata atccatgccg tggatcagat cacgcaccgc ggcaatcggc tcccgctccg
    24181 ccagacgctg gatttctgcc aaccagtgag tgaagcgggt caatgcttca taaccacgtc
    24241 cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg
    24301 tcatcgccca ttcacccaac tttttcagcg tcgccgggcc aatctctcgc ttcggcgtgt
    24361 taacgatacg caggaacgca ctatcatcgt ccgggttagt cagcacgcgc agataagcca
    24421 gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accagaaatt ttgtacggga
    24481 tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg cgataaagaa
    24541 tggcgtaatc tttgtactgc gttttattga cgaagtgatg ggcaatcagc tcgccagtaa
    24601 cgcgctcggc ttcatgttcc tcgttattcg cgcttaaaac ttttagctct gtgccgtaac
    24661 ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc aggatgttcg
    24721 ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc accttcagcg
    24781 ccggaaaatc ctgactcagc agcaccaggt tttgcggacg tgcgccgcgc caggagtaga
    24841 tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc agcagtttca
    24901 ccagctcata ctggctggtg ttggtgtcct gatattcgtc caccagcagg tagcgaattt
    24961 tgttctgcca gcgctcgcgg acttcttcat tgcgttgcaa cagcaacgtt ggcaataaaa
    25021 tcagatcatc gaaatcgaga acgttacagg ctttcaggtg tgcatcatac agcccataac
    25081 aatgggcaaa aatacggtcc cgctcgccct ttgcctctgc cgccgcctgc gccggtgttt
    25141 tgagatcgtt cttccagttg gagatggtcg aaattagctg ttgcaggaga actttgtcat
    25201 cttcaatcag cccctcggtc aactctttaa gcaaagcaag ctgatcggta tcgtcaaaca
    25261 gcgaaaagtt agccttcatc cccaacgccg cgtactcacg cttaatgata tccagcccca
    25321 gcgtgtgaaa ggtggagatc atcagcccac gcgcctcttt acgccccagc gtctgcccga
    25381 cacgctcttt catctcgcgt gccgccttat tggtaaaggt caccgccgcg atatgtcgcg
    25441 cctgataacc gcaaccgcgg atcagatggg cgattttatt ggtgataaca cgagttttac
    25501 cggaacccgc gcccgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt
    25561 ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag tcgggagggg aaaataacag
    25621 cgtggtagta tagccagcct catccttacc actcaaggca cgatcatggc aaaaacagca
    25681 gcagcactgc atatccttgt aaaagaagag aaactggctc tggatcttct cgagcagatc
    25741 aagaatggtg ccgatttcgg caagctggcg aagaaacact ccatttgccc gtcaggcaaa
    25801 cgcggcggtg atttgggtga attccgccag ggtcaaatgg ttccggcgtt cgataaagtg
    25861 gttttctctt gtccggtact ggagccaacc ggcccgctgc atacccagtt cggttaccac
    25921 atcattaagg tgctgtaccg taactaacag caaggccttc tccgggagaa ggccttgaac
    25981 tctgaatcgc tattaacccg caacagcaat acgtttcata tccgtcatat agccgcgcag
    26041 tttcttacct acctgctcaa tcgcatggct gcgaatcgct tcatttacat cacgcagctg
    26101 cgcgttatct accgcacctt ccggaatagc tttacccagg tcgcccggtt gcagctctgc
    26161 cataaacggt ttcagcagcg gcacacaagc gtaagagaac agatagttac cgtactcggc
    26221 agtatcggag ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat
    26281 cagcggcaat tcgtgcagtg attcatagta agcagattct tcgatgatgc cggaatcaac
    26341 catggtttca aacgccaact caacgcctgc ttttaccatc gcgatcatca gtacgccttt
    26401 atcgaagtac tcctgttcgc cgattttgcc ttcatactgc ggcgcggttt cgaatgcggt
    26461 tttgccagtc tcttcacgcc aggtcagcag tttcttatcg tcgttggccc agtcagccat
    26521 catgccggag gagaattcgc cggagatgat gtcgtccata tgtttctgga acagcggcgc
    26581 catgatctct ttcagttgct cagagagcgc gtaagcacgc agtttcgccg ggttagaaag
    26641 acggtccatc atcagggtga tgccgccctg tttcagcgct tcggtgacgg tttcccaacc
    26701 gaactgaatc agtttttctg cgtatgccgg atcggtgcct tcttccacca gcttgtcgaa
    26761 gcacagcaga gaaccagctt gcaacatacc gcacaggatg gtttgctcgc ccatcaggtc
    26821 agatttcact tccgcaacga aagaggattc cagcacgccc gcacggtgac cgccggttgc
    26881 agccgcccat gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt ccgggtgaac
    26941 ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgtactt cggtgccagg
    27001 gcatttcggc gcaaccatta cgacggtgat gtctttacgg atctgctcgc ccacttcgac
    27061 gatgttgaaa ccgtgtgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt
    27121 gcgtactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgcgg
    27181 gatcagttct tcgtaagtac ccactttaaa accattttcg gttgctttac gccaggatgc
    27241 gcgcttctcg gcaatcgctt ctttacgcag agcgtaagaa atatccagac cagaatcacg
    27301 catgttcagg ccctggttca gcccctgtgc gccacagccg acgatgacta cttttttacc
    27361 ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac atttgcccag
    27421 ctgtgccagc tgctggcgca gattcagtgt attgaagtag ttagccatgg tgattcctcg
    27481 tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac tatatgacag
    27541 gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct gcaacgtcaa
    27601 cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    27661 gccgccattt tggccgtagc gcgcgggcaa tgcacgttag cccgtctacg ctttcgcgac
    27721 aaatacagcg cctggaagaa gatctcggtc agccgctgtt tgtgcgtgat aaccgcacgg
    27781 taacgctgac ggaagcgggc gaagagctgc gcgttttcgc ccagcaaacg ctgttgcagt
    27841 atcagcaatt gcgccacacc atcgaccagc aagggccgtc gctctctggc gaattacata
    27901 tcttctgctc ggtgaccgct gcctacagcc atctgccgcc gattctggat cgcttccgcg
    27961 cggaacatcc gtcagtggag atcaaactct ctaccggtga tgcggccgat gcgatggaaa
    28021 aggtggtcac tggcgaagcg gatctggcga ttgccgggaa accggaaacc ttgccgggcg
    28081 cagtggcgtt ttcgatgctg gagaatctgg cggtggtgct gattgctccc gcgctgccct
    28141 gcccggtgcg taatcaggta tctgtcgaaa agcccgactg gtctacagtg ccgtttatca
    28201 tggccgatca gggaccggta cgtcgccgca ttgaactgtg gtttcggcgt aacaaaatca
    28261 gcaacccaat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    28321 tcggctgtgg cgtggcgctg ttgccggaag tggtgctgga taacagcccg gaaccggtgc
    28381 gtaaccgcgt gatgatttta gagcgcagtg atgaaaaaac gccgtttgag ctgggcgtgt
    28441 gcgtacaaaa aaagcggctt catgagccgc tgattgaggc gttctggaag attttgccga
    28501 accactaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg tggcaatcgt
    28561 agcccagttc attcagccgg gtttcaaaat ccggttcatg atcgccaagc tcgaacgccg
    28621 ccagtacgcg cccgtagtcg gtaccgtggc tgcgataatg gaacagcgag atgttccagt
    28681 gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg cgattccggg aattcgaagc
    28741 tgtagaggcg ttcctgcaac ggatgcgatg gacgcccgcc aaccatatag cgcacatgca
    28801 gcttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccatcg ttgagcatct
    28861 gcaaaatttc tttgcgctct tcgaggccac ggctcaggcg cacgccgaca aaaatgcagg
    28921 cgtttttggc atcagcaaag cggtagttga actcggtgac cgagcgccca ccaagtaact
    28981 ggcagaattt gaggaagctg cccttttctt ccggaatggt caccgccagt aacgcttcac
    29041 gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag accgtgaaag ttcacgttgg
    29101 caccggaaag aatatgcgcc agccgctcac cgcgaatgtt gtgctgggcg atgtattttt
    29161 tcatccctgc cagcgccagc gcgccagagg gttcagccac cgcgcgtaca tcttcgaaca
    29221 ggtctttcat cgccgcacag atggcatcgc tatcgacggt gatgatgtcg tcaagatact
    29281 cctggcacaa acggaaggtt tcatcgccga tgcgttttac cgcgacgcct tcagcaaaca
    29341 gccccacgcg gggcagatca acgggatgac ctgcatcaag tgccgctttc aggcaggcgg
    29401 aatcttccgc ttctacggcg atcactttga tttgtggcat cagttgtttg atcagcaccg
    29461 ccacgcccgc cgccagaccg ccgccgccga ccggaacaaa tacgcggtcg agatgagcat
    29521 cctgctggag cagttccagc gccagcgtac cctgaccagc gatcaccatc ggatgatcga
    29581 agggtggcac ccaggtgaag ccctgctgct gtgacagttc gattgctttc gctttcgctt
    29641 cgtcaaagtt cgcgccatga agcagcactt cgccgccaaa gccgcgcacc gcatcaactt
    29701 tgatatcggc ggtggcggtt ggcatgacga tcagcgcctt cacgcctaac cgtgcggaag
    29761 aaaacgcgac gccctgcgcg tggttacccg cagaagcggt aatcacgccg tgcgcttttt
    29821 gttcttccgt caggcccgcc atcatggcgt atgcaccacg aagcttaaag ctgtgcactg
    29881 gctggcgatc ttcgcgcttc accagaatca cgttatcaag acgcgacgac agtttttcca
    29941 ttttctgtag cggcgtgacc tgcgccgctt cgtagaccgg tgcgcgcagc accgctctta
    30001 aatattcggc accttccggg gcaccggaca ggggttgtga gtctgccatc attaaccccc
    30061 cagtttcgat ttatcgcgca ccgcgccttt gtcggcgctg gttgccaggc tggcgtaggc
    30121 gcgcagggca aaggagacct gacgttcacg gtttttcggc gtccaggctt tgtcaccccg
    30181 agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta actgaatacc
    30241 acggttcgga atgtcgatgg cgatcaggtc gccgtcttca atcaggccaa tgctgccgcc
    30301 gcttgccgct tccggtgaga cgtggccgat agaaaggcca gaggtaccgc cagagaaacg
    30361 accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga agctggttgg
    30421 gtagagcatt tcctgcatcc ccggaccgcc tttcgggcct tcatagcgaa ttactaccac
    30481 atctccggcg acaactttac cgccgagaat cgcttctacc gcatcgtcct ggctttcgta
    30541 cactttcgcc gggccggtga atttgaggat gctgtcatcc acgcccgcgg ttttaacgat
    30601 gcagccgttt tccgcaaagt taccgtagag caccgccagg ccgccgtctt tgctgtaggc
    30661 gtgttccagc gagcggatac agccattggc gcgatcgtcg tccagcgtat cccaacggca
    30721 atcttgcgag aatgcctggg tggtacggat acccgcaggg ccggcgcgga acatattttt
    30781 taccgcgtca tcctgggtca gcataacgtc gtattgttcc agcgtttgcg gcaacgtcag
    30841 gccgagcacg tttttcacat cacggttcag taaccccgcg cgatccagtt cgccgagaat
    30901 accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttctggg tgctcggcgc
    30961 aactttacac agctgtggaa ccttgcggga aagcttatcg atatcactca tggtgaagtc
    31021 gatttccgct tcctgcgccg ccgccagcag atgaagtacg gtgttggtag agccgcccat
    31081 cgcgatatcc agcgtcatgg cgttttcaaa cgccgcctta ctggcgatat tacgcggcag
    31141 tgcactatcg tcgttttgct cgtaataacg tttggtcaat tcaacaatgc gtttaccagc
    31201 attgaggaac agctgcttac ggtccgcgtg ggttgccagc aacgagccgt tgcccggctg
    31261 cgacaggccc agtgcttcgg tcaggcagtt cattgagtta gcagtaaaca tcccggagca
    31321 ggaaccgcag gttgggcagg cggatcgttc aacctgatcg ctctgggagt cagaaacttt
    31381 cgggtctgca ccctggatca tcgcatcaac cagatcgagc ttaatgatct gatcggacag
    31441 tttggttttc ccggcttcca tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg
    31501 cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc agaccatggc
    31561 gtcggcgcag tgagcgttga ccatatactc aacggaatca gcgatcaatt cgcgagatgg
    31621 cagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg caatggtgtt
    31681 gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt taccgagatc
    31741 gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg
    31801 cttaccgaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag cacccgccat
    31861 attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt tactcccagt
    31921 gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt
    31981 gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag aaggcttgct
    32041 gaatgcgttt ggtaaccggg ccgcaacggc cttcaccaac ctgaataccg tctacactgc
    32101 gcactggcgt gatttctgcc gcagtaccgg acataaacac ttcatccgcc aggtacagtg
    32161 attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc agtttgatga
    32221 tggcgtcacg ggtaataccc ggcagcgcgg atgaggtgaa cggtggggtg aacagcacac
    32281 cgtctttcac ttcaaacagg ttttcgcctg cgccttcaga gatgtaaccg ttcacatcca
    32341 gcgcgatacc ttcctgataa ccgtggcggc gtgcttcact acccaccagc agggaagaga
    32401 ggtagttacc accggctttt gccgcggttg ggatggtgtt tggtgctgcg cggttccagg
    32461 aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac gccccccacg
    32521 ggaaagcggc gataatcacg tcggttgagt atcccgctgg cgggtttacg cccatgccaa
    32581 catcaccaac gaagatcagc ggacggatat aggcgctggt gagattgttt ttgcggatca
    32641 cgtcacgaca agcttccatc agctcatcaa tgctctgcga aaccgggaag cgatagattt
    32701 tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc ggtcctttgt
    32761 gcgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc gcgtgcgaca
    32821 tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa atgtaatcag
    32881 ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg ttgtgatgtg
    32941 gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa ctgactaaac
    33001 agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt ttgtgcatcg
    33061 ctggcggcgg ccatattcat tgaacagacg tggaaaccac gatgacgcac cacgcgtaaa
    33121 acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg ttgcatcatg
    33181 ataatttctc caacatttct gaattactgg caccaggcgg caccagcggc cagacgttct
    33241 caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc atggtgtcga
    33301 gtgccgcttc aacctggtct ttatgggtga tgtgttggcc agggatgccg aaggcgctgg
    33361 ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt tcctggaaaa
    33421 acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag acgattttca
    33481 acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg aaagagccgt
    33541 caccggagat acagacgaca gtatcgttcg gtcgcgcgac ttgtgcgcca accgccgccg
    33601 gtaaaccgaa acccatggtg cctaagccgc tggaggtgat gaagttttcc gggcgagtgt
    33661 ggacgatgtg ctgggcagcc cacatctggt gctgccccac atctgtggtc acgatgcaat
    33721 ccgcagattt acgatccgac agttgtttta acaacaacgg cgcgtagata gcgtcaccag
    33781 gatggtcgta acgccaggca tgttcatcac gcagttgcgc gcagtgttgc tgccagtcat
    33841 cgatatttaa cggctgctgt aatgctggta acagagcatt taaatcaccc tgtaatgcca
    33901 catgtgcctg acgcagcttg ttcatttctg ccggatcgat atccatatgg ataacgctgg
    33961 cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt gcgcccacgg
    34021 cgatcagtag atcgcactcc tgcactgcga agttcgccgc tttggtgccg tgcatcccca
    34081 gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc aacgtacagg
    34141 tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cgaaactgcc tgcgccatgc
    34201 ccacgccacc accaacgtac agcatcggtt tttgcgcttt tgccagcatc tgacgcgctt
    34261 gctcgacttc ggcatgtggg aaagtcactt cgttttcaac agtggtgaac cacggttcca
    34321 gatcgccgct ggctaactgg atatcttttg gaatatcgac caaaaccgga ccaggacgac
    34381 cagagctggc gacgtcaaac gcttcggcca tgatacgcgg taactcttcc agtgattgca
    34441 ccaggaaact gtgcttggtg caggctaacg acaaccccag aatatccacc tcctgaaatg
    34501 cgtcagtgcc gataaacggt gcggatacct gaccggtgat agcaacaacg gggatggaat
    34561 ctaacagcgc gtccgcaagc ccggttatca ggttggttgc acctggacca gacgtggcga
    34621 tgcaaacgcc cgttttgccg gtagcacggg cataaccgat agccgccatt gctgcacctt
    34681 gctcgtgtcg gcacagcaag tgttccacgc caccgtcata caatgcatcg taaaccggca
    34741 taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc aacgcatgta
    34801 ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga aacagaattt
    34861 tgtgctgtta ttcattgtct gctcctcagt tatgttttta aggtcaaaaa aaacccccgg
    34921 acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt tcctcgtcca
    34981 agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac caggctaatc
    35041 actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga atgcctaaag
    35101 agttaccata gattttgcga atagcacaag atatt
//
LOCUS       DAZGTJ010000048        34519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000048 DAZGTJ010000000
VERSION     DAZGTJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.048"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..684
                     /locus_tag="ACHK8E_002839"
     CDS             <1..684
                     /locus_tag="ACHK8E_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP0345079.1"
                     /translation="ITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAVRR
                     TLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKASASLAALWQVVNQIGGK
                     ERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTLLF
                     GMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKRI
                     ARLAEQSTD"
     gene            783..1796
                     /gene="ybjS"
                     /locus_tag="ACHK8E_002840"
     CDS             783..1796
                     /gene="ybjS"
                     /locus_tag="ACHK8E_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0345080.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1793..2623)
                     /gene="amiD"
                     /locus_tag="ACHK8E_002841"
     CDS             complement(1793..2623)
                     /gene="amiD"
                     /locus_tag="ACHK8E_002841"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP0345081.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2620..2943)
                     /gene="ybjQ"
                     /locus_tag="ACHK8E_002842"
     CDS             complement(2620..2943)
                     /gene="ybjQ"
                     /locus_tag="ACHK8E_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP0345082.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3069..3584
                     /gene="ybjP"
                     /locus_tag="ACHK8E_002843"
     CDS             3069..3584
                     /gene="ybjP"
                     /locus_tag="ACHK8E_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0345083.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3802..4530
                     /gene="artP"
                     /locus_tag="ACHK8E_002844"
     CDS             3802..4530
                     /gene="artP"
                     /locus_tag="ACHK8E_002844"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP0345084.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4548..5279
                     /gene="artI"
                     /locus_tag="ACHK8E_002845"
     CDS             4548..5279
                     /gene="artI"
                     /locus_tag="ACHK8E_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP0345085.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5286..6002
                     /gene="artQ"
                     /locus_tag="ACHK8E_002846"
     CDS             5286..6002
                     /gene="artQ"
                     /locus_tag="ACHK8E_002846"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP0345086.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6002..6670
                     /gene="artM"
                     /locus_tag="ACHK8E_002847"
     CDS             6002..6670
                     /gene="artM"
                     /locus_tag="ACHK8E_002847"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP0345087.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFIGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6896..7627
                     /gene="artJ"
                     /locus_tag="ACHK8E_002848"
     CDS             6896..7627
                     /gene="artJ"
                     /locus_tag="ACHK8E_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP0345088.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7656..8783)
                     /gene="rlmC"
                     /locus_tag="ACHK8E_002849"
     CDS             complement(7656..8783)
                     /gene="rlmC"
                     /locus_tag="ACHK8E_002849"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP0345089.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(8824..9312)
                     /gene="ybjO"
                     /locus_tag="ACHK8E_002850"
     CDS             complement(8824..9312)
                     /gene="ybjO"
                     /locus_tag="ACHK8E_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP0345090.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9372..10217)
                     /gene="potI"
                     /locus_tag="ACHK8E_002851"
     CDS             complement(9372..10217)
                     /gene="potI"
                     /locus_tag="ACHK8E_002851"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP0345091.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(10214..11167)
                     /gene="potH"
                     /locus_tag="ACHK8E_002852"
     CDS             complement(10214..11167)
                     /gene="potH"
                     /locus_tag="ACHK8E_002852"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP0345092.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11177..12310)
                     /gene="potG"
                     /locus_tag="ACHK8E_002853"
     CDS             complement(11177..12310)
                     /gene="potG"
                     /locus_tag="ACHK8E_002853"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP0345093.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12405..13517)
                     /gene="potF"
                     /locus_tag="ACHK8E_002854"
     CDS             complement(12405..13517)
                     /gene="potF"
                     /locus_tag="ACHK8E_002854"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP0345094.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(13868..14344)
                     /gene="ybjN"
                     /locus_tag="ACHK8E_002855"
     CDS             complement(13868..14344)
                     /gene="ybjN"
                     /locus_tag="ACHK8E_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP0345095.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14432..15334)
                     /gene="rimK"
                     /locus_tag="ACHK8E_002856"
     CDS             complement(14432..15334)
                     /gene="rimK"
                     /locus_tag="ACHK8E_002856"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP0345096.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15395..16117)
                     /gene="nfsA"
                     /locus_tag="ACHK8E_002857"
     CDS             complement(15395..16117)
                     /gene="nfsA"
                     /locus_tag="ACHK8E_002857"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP0345097.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16101..16388)
                     /gene="ybjC"
                     /locus_tag="ACHK8E_002858"
     CDS             complement(16101..16388)
                     /gene="ybjC"
                     /locus_tag="ACHK8E_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP0345098.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16548..16805
                     /gene="grxA"
                     /locus_tag="ACHK8E_002859"
     CDS             16548..16805
                     /gene="grxA"
                     /locus_tag="ACHK8E_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP0345099.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(16835..17212)
                     /gene="ybjM"
                     /locus_tag="ACHK8E_002860"
     CDS             complement(16835..17212)
                     /gene="ybjM"
                     /locus_tag="ACHK8E_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP0345100.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17482..19167
                     /gene="ybjL"
                     /locus_tag="ACHK8E_002861"
     CDS             17482..19167
                     /gene="ybjL"
                     /locus_tag="ACHK8E_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP0345101.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19343..19879)
                     /gene="rcdA"
                     /locus_tag="ACHK8E_002862"
     CDS             complement(19343..19879)
                     /gene="rcdA"
                     /locus_tag="ACHK8E_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP0345102.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            19963..21171
                     /gene="ybjJ"
                     /locus_tag="ACHK8E_002863"
     CDS             19963..21171
                     /gene="ybjJ"
                     /locus_tag="ACHK8E_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345103.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21171..21986
                     /gene="ybjI"
                     /locus_tag="ACHK8E_002864"
     CDS             21171..21986
                     /gene="ybjI"
                     /locus_tag="ACHK8E_002864"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP0345104.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            22071..22355
                     /gene="ybjH"
                     /locus_tag="ACHK8E_002865"
     CDS             22071..22355
                     /gene="ybjH"
                     /locus_tag="ACHK8E_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP0345105.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22396..23628)
                     /gene="mdfA"
                     /locus_tag="ACHK8E_002866"
     CDS             complement(22396..23628)
                     /gene="mdfA"
                     /locus_tag="ACHK8E_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP0345106.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            23913..24509
                     /gene="ybjG"
                     /locus_tag="ACHK8E_002867"
     CDS             23913..24509
                     /gene="ybjG"
                     /locus_tag="ACHK8E_002867"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0345107.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            24567..25325
                     /gene="deoR"
                     /locus_tag="ACHK8E_002868"
     CDS             24567..25325
                     /gene="deoR"
                     /locus_tag="ACHK8E_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP0345108.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25372..26574)
                     /gene="dacC"
                     /locus_tag="ACHK8E_002869"
     CDS             complement(25372..26574)
                     /gene="dacC"
                     /locus_tag="ACHK8E_002869"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0345109.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            26821..27447
                     /gene="gstB"
                     /locus_tag="ACHK8E_002870"
     CDS             26821..27447
                     /gene="gstB"
                     /locus_tag="ACHK8E_002870"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP0345110.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27444..28559)
                     /gene="yliI"
                     /locus_tag="ACHK8E_002871"
     CDS             complement(27444..28559)
                     /gene="yliI"
                     /locus_tag="ACHK8E_002871"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP0345111.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28670..29053)
                     /gene="bssR"
                     /locus_tag="ACHK8E_002872"
     CDS             complement(28670..29053)
                     /gene="bssR"
                     /locus_tag="ACHK8E_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP0345112.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29266..30591
                     /gene="rimO"
                     /locus_tag="ACHK8E_002873"
     CDS             29266..30591
                     /gene="rimO"
                     /locus_tag="ACHK8E_002873"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP0345113.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            30817..31203
                     /locus_tag="ACHK8E_002874"
     CDS             30817..31203
                     /locus_tag="ACHK8E_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0345114.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            31193..31522
                     /locus_tag="ACHK8E_002875"
     CDS             31193..31522
                     /locus_tag="ACHK8E_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0345115.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(31592..32920)
                     /gene="dgcI"
                     /locus_tag="ACHK8E_002876"
     CDS             complement(31592..32920)
                     /gene="dgcI"
                     /locus_tag="ACHK8E_002876"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0345116.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32928..34519)
                     /locus_tag="ACHK8E_002877"
                     /pseudo
     CDS             complement(32928..34519)
                     /locus_tag="ACHK8E_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         8706 a         8922 c         8412 g         8479 t
ORIGIN      
        1 attacttccg taccgcccac caccgccagg gcgttgattc aggggctgaa acatgatctg
       61 ctggcggatg acaccgcgct acgtgcactc atcccacaac ggctgatcgc tttcgatgac
      121 gcggtacgtc gcaccctgaa agaagaagaa aagctggtca actccagcga ctggggctac
      181 gacgctcagg cctttgcccg ctggcgaccg gagtacggtt atttcgccaa acaggcgggg
      241 tttaccgtta aagcgtccgc cagccttgcg gctttatggc aggtggtgaa tcaaatcggc
      301 ggtaaagagc gttatttctt tggcaatatt ttgtggcaga cacgggcgtt gatggaccgc
      361 gcgatcggtc ataagctggc gaaaggccgc ccggagcgtg aatatttgca gaccggcgat
      421 gcggtggata gctggaaagt gattgtcgtt gaaccggaaa aacaacttac gctgttattt
      481 ggcatgaaag cgccggggct gggacgactg tgttttaccc tggaagataa aggtgactat
      541 cgtactatcg atgttcgcgc attctggcat ccgcacggta tgccggggct gttttactgg
      601 ttattgatga tccccgcgca tctgtttatt tttcgcggaa tggcaaaacg aatcgccaga
      661 ctggcagaac aaagcacaga ttaatcgaca aaacctgcgt tattctttca tgtttcccat
      721 tgcatcatgg gggaaatcac ggaagaatgc gcacgttatt ttctcaacaa cagtggattg
      781 atatgaaggt actggttacc ggcgccacca gcggcttagg tcgaaacgcg gtagagtttt
      841 tatgccagaa aggcatcagc gtgcgagcga ccggtcgcaa cgaggcaatg ggtaaattgc
      901 tggaaaaaat gggcgcagag tttgttccgg cggatctgac cgagctggtc tcatcacaag
      961 ctaaagtgat gctcgcgggc attgatacgc tgtggcactg ctccagcttt acctcgccct
     1021 gggggacaca acaggctttc gatctggcta acgttcgcgc cactcgccgc ctgggtgaat
     1081 gggctgtcgc ctggggtgtg cgtaacttta ttcatatctc ttcaccctcc ctgtacttcg
     1141 attatcacca ccatcgcgat attaaagaag attttcgccc tcaccgcttc gccaacgagt
     1201 ttgcccgtag caaagcagcc agcgaagagg tgatcaatat gctttcgcag gcgaatccac
     1261 aaacgcgctt tactattctg cgcccacaaa gtctgttcgg accgcacgat aaagtcttta
     1321 ttccccgtct ggcgcatatg atgcaccact acggcagcat tctgttaccg catggcggca
     1381 gtgcgctggt cgatatgacc tactatgaaa atgccgtgca cgccatgtgg ctggcaagcc
     1441 aggaagcctg cgataagcta ccttccgggc gtgtgtacaa catcaccaac ggcgagcatc
     1501 gcacactgcg cagcatcgtg cagaagctga tcgacgagtt gaatattgac tgtcgtattc
     1561 gttccgtccc ctacccgatg ctggatatga tcgcccgcag catggagcgt ttaggccgca
     1621 agtcagcaaa agagccgccg ctgacccact acggcgtctc caagttgaat tttgacttta
     1681 cgctggatat tacgcgcgcg caggaagagt taggttatca gccggtcatc accctggatg
     1741 aaggtatcga gaaaactgcc gcctggctac gcgaccacgg aaaactgccg cgctaatcct
     1801 gcccgtattt ctccagcaac gcttcggcaa tcgcctgagt ttctgcatcc gcttcgccgt
     1861 tgtataacgt cgggcggaaa tgcatctgga acgccataat cacgcgccgc tgctcgcgcg
     1921 gtgtcatatc aggtttaacg tcataaccgt agcgcgccaa aagctccagt aatgatgcgg
     1981 tatctaccag tgtgtgcggc gcgcgcccgg caaggtaaaa gttcacccgc tgcgcatccg
     2041 gccaggcacc aatcccctgc tgcgccagtt gttgccaggg gaataacggc cccggatcgt
     2101 ctttgcgctg cggcgcaata tccgcatgcg ccactacgtt ttccggcttg atgtgataac
     2161 gggcaataat atctttcgcc agcggaataa gcgcctgaat ctgtgcctgt tcaaacgggg
     2221 caaaatattt cactccggat gatttttgcc agccacggtt ttccagctca atgccaatag
     2281 aggtgtcgtt aagacgcgtt gccccgcgcc aggcgctaat ccccgcatgc caggccagtt
     2341 cctgttccgg caccagttgc cagatgcgcg gtttaccgtt gtatcgcggt ggtaccgcag
     2401 ggaccagata atgcgagctg acctgcttat cggtcagtgt cgccagcgag ctatcaaaat
     2461 catctgcggt gtagtggata accagcactt taatgcgcgg atacgccgcc tgcgcctggc
     2521 gtcgggtatc aagctgatat ccctctttct cgacaatgcc tttttcgcct gcacaccctg
     2581 ccagtaacag agcgacagcg accagccaaa agactcttct catcgacgcg ttttcaccgc
     2641 cgtaccgctg acgctaacca tcagcatact gccattttgc ccgaccgttt cgtagtcgat
     2701 atcaataccg actacggcat cagcccccag cgcccgggcc tgatcgccga gctcctcaaa
     2761 ggcgatctcc cgcgctttac gcaattcttt ttcataagcc ccggagcgtc cgccaacgat
     2821 atcgcggata ccggcaaaga aatcacggaa aatattggca cctaaaatcg cttcgccggt
     2881 caccacaccg caatattcga cgatggtctg gccttccaga gttggggttg tagaaaattg
     2941 catgatcgat ctccttgttt gctaagcctt cgatcttcaa agcattatcg gactgatacg
     3001 ctattattga aaggatatca ttattatcgt agaacgcttt caaggtgttc gcttataagg
     3061 aaatcatcat gcgctatagc aaattgacca tgcttatccc ttgcgcactg ctactcagtg
     3121 cctgtaccac cgtcacgcca gcttataaag ataacggccc ccgcaccggt tcctgtgtcc
     3181 agggggggcc tgatagcgtg gctcaacagt tctatgacta ccgcattcaa catcgcggca
     3241 atgacattac cgctctgcgc ccctatttga gtgacaaact ggcaacccta cttagcgatg
     3301 ccagccgcga taatagtcat cgtgaattgt taagcagcga tccgttctcc agtcgaacca
     3361 ccttgccaga cagcgcccac gttgccagtg catcgactat ccctaatcgt gatgcccgta
     3421 acattccatt gcgcgtggat ctcaagcagg gtgaccaggg ctggcaggat gaagtgttaa
     3481 tgattcagga aggccagtgt tgggtcatcg acgatgtgcg ctacttaggc ggtagcgttc
     3541 atgccacagc gggtacgctc cgtcagtcca tcgagaatcg ctaacgtctt gatcaaccaa
     3601 taaaccccgt aaattgtacg gcatttcgtg aggaatgccg acatttatgc tcgccgacca
     3661 ccgcccccgt tattttgtgc tatgtttatt gaataatgcg ctttgctttt aacttttaaa
     3721 gcagaaatat tgcataatta ttctgtcaaa ggtactatct tcgacctcaa tagctatcag
     3781 actgccagta tacgagtgtc aatgagtatt caattaaacg gcattaattg cttctacggc
     3841 gcgcatcagg cgctgttcga tatcacgctg gattgcccac agggcgaaac gctggtgtta
     3901 cttggcccca gcggcgcggg taaaagctcg ctgctgcgtg tactcaatct gcttgagatg
     3961 ccgcgctccg gtacgctcaa cattgcaggc aaccatttcg atttcaccaa aaccccctct
     4021 gacaaagcga ttcgcgattt gcgtcgtaac gttggcatgg tgtttcagca atacaacctg
     4081 tggccgcatc tgaccgtgca gcaaaacctg attgaagcgc cctgtcgtgt actggggttg
     4141 agtaaagatc aggcgctggc ccgtgcagaa aaactgctgg aacgtctgcg tctcaaacct
     4201 tatagcgatc gttacccgct gcatctttct ggaggtcagc agcagcgtgt tgctattgcc
     4261 cgtgcgttga tgatggaacc gcaggtactg ctgttcgatg aaccgaccgc cgcactggac
     4321 cccgaaatta cggcacaaat cgtcagcatc attcgtgagc tggcagaaac gaatattacc
     4381 caggtgatcg tcacccacga agttgaagtg gcgcgtaaaa ccgccagccg agtggtgtat
     4441 atggaaaacg gtcatatcgt agaacaaggc gacgcgagct gctttaccga gccgcaaacc
     4501 gaagcattta aaaactatct ctctcactaa gattcgggat aacaacaatg aaaaaagttc
     4561 tgattgccgc gttaattgca ggttttagtc tttccgccac agcagccgaa accattcgtt
     4621 ttgccaccga agcctcctat cctccgtttg aatcgattga tgcaaacaac cagatcgttg
     4681 gttttgatgt cgacctggcg caggcattgt gtaaagagat tgatgcaacc tgcactttct
     4741 ctaaccaggc gtttgacagc ctgatcccaa gcctgaaatt ccgtcgcgta gaagcagtga
     4801 tggcgggcat ggatatcact ccggagcgtg aaaagcaggt gctgtttacc accccgtact
     4861 atgacaactc tgccctgttt gtgggtcagc aaggcaaata caccagtgtt gatcagctga
     4921 aaggcaaaaa agtcggcgtt cagaacggga cgacacacca gaagttcatt atggataagc
     4981 atccggaaat cactaccgtg ccatatgaca gttaccagaa cgcaaaactg gatctgcaaa
     5041 acggtcgtat cgacagcgta tttggtgaca ccgcagtggt aaccgaatgg ctgaaagata
     5101 acccgaaact ggcggcagtg ggcgataaag tgaccgataa agattacttc ggtactggcc
     5161 tcggcatcgc agtacgccag ggcaacactg aactgcagca gaaactcaac actgcgctgg
     5221 aaaaagtgaa gaaagatggc acttacgaaa ccatctacaa caaatggttc cagaagtaat
     5281 tcctgatgaa tgaatttttt cctttagcaa gcgccgccgg gatgaccgtc ggccttgccg
     5341 tttgtgcatt gattgtcggg ctggcgctgg cgatgttctt tgcggtatgg gagtctgcaa
     5401 aatggcgtcc tgtcgcgtgg gcaggttcag cgttggtgac ggttctgcgc ggcctgccag
     5461 aaattctggt ggtgctgttt atctattttg gctcctcgca gctgctgctg acgctttcgg
     5521 atggcttcac tatcaatctt gggttcgtgc agatcccggt gcagatggac attgagaact
     5581 tcgacgttag cccgttcctt tgtggtgtca tcgctctgtc actgctgtat gccgcctatg
     5641 cctcgcaaac gctgcgcggc gcgttgaaag cggtgccggt gggccagtgg gaatctggtc
     5701 aggcgctggg gctgtcgaaa tcggctatct ttttccgtct ggtgatgccg cagatgtggc
     5761 gtcatgcgct gccgggtctc ggtaaccagt ggctggtgct gctgaaagat accgcgctgg
     5821 tcagtttgat tagtgtgaat gatttaatgc tacaaaccaa aagcatcgct acccgtaccc
     5881 aggaaccgtt tacctggtac attgtggcgg cggcgattta cctggtgatc accctgctca
     5941 gtcagtacat tctcaaacgc attgacctgc gcgcgacacg ttttgagcgg aggcccagct
     6001 aatgtttgag tatttacccg aactgatgaa agggttgcac accagcctga cgctaaccgt
     6061 tgcctcgctg attgtggcac tgattctggc attgattttt accatcatcc tgacgctgaa
     6121 aacgccggtg ctggtgtggc tggtgcgggg ctatatcacg ctgtttatcg gtacgccgct
     6181 gctggtgcag atcttcctga tttattacgg accgggccag ttcccgacgt tgcaggagta
     6241 tccggcactg tggcatttgt tgtcagaacc gtggctgtgt gcgctgattg cgttgtcgct
     6301 gaacagtgcg gcgtatacca cgcagctgtt ttacggtgca attcgtgcga tcccggaagg
     6361 tcagtggcag tcctgtagcg ccctgggaat gagcaaaaaa gatacgctgg cgatcctgct
     6421 gccgtatgcc tttaaacgct cgctctcttc ttattccaac gaagtagtgc tggtgttcaa
     6481 aagtacctcg ctggcctaca ccattacgct gatggaagtg atgggataca gccagttgtt
     6541 gtacggacgc acctacgatg tcatggtgtt cggcgcggca gggattattt atctggtcgt
     6601 taacggcctg ctgacgctga tgatgcgtct gatcgagcgt aaagcgctgg cgtttgagcg
     6661 aaggaattaa tttggctgcg tgctgatgcc ggatgcggcg cggacgcctt atccggccta
     6721 catagtgcat aaattcttat aatgaagacg gacaactcac taagttgtcc gtcttttttt
     6781 atttcattta aattatttaa tcatatttat tgcatataaa ttcacttgat ggcattgtta
     6841 tcccatgccg cagacacggc caaaaatcat aagattgaca gacgggagtt ccatcatgaa
     6901 aaagttagtt cttgccgcat tacttgcttc ctttactttc ggtgcttctg ccgcagagaa
     6961 aatcaatttt ggcgtttcag ccacctatcc accctttgaa tctataggtg ctaataatga
     7021 gattgtcggc tttgatatcg acctggcaaa agccttgtgc aaacaaatgc aggcagaatg
     7081 tacttttact aatcacgcgt tcgacagcct gatcccgtca ctgaaattca gaaaatatga
     7141 cgccgtaatc tccggtatgg atatcactcc agagcgtagc aaacaggtat cgtttaccac
     7201 gccctactat gaaaactcag ccgtcgtgat tgccaaaaaa gatacctaca aaacgtttgc
     7261 cgatctgaaa ggcaaacgta ttgggatgga aaacggtact acgcaccaga aatatattca
     7321 ggatcagcac ccggaagtga aaactgtctc ctatgacagt tatcagaatg cctttatcga
     7381 tctgaaaaat ggccgtattg atggggtatt tggtgacaca gcggtggtta atgaatggct
     7441 gaaaaccaac ccgcaactgg gcgttgcgac tgaaaaagtg accgatccgc aatacttcgg
     7501 caccggcctg ggtatcgctg tacgtccgga taacaaagct ctgctggaaa aactgaataa
     7561 cgcgctggca gcaattaaag ctgacggtac atatcagaaa atcaatgacc agtggttccc
     7621 gcagtaagat tcagaaagcc gcacctgcgg cttttttatt gcttaaccag cagtgtcagc
     7681 acttcgtaat gcgcagtgtg cgggaacata tcaaaaagct gtacccgttc gatgcagtaa
     7741 cctggcagtt cgcggatatc tttcgccatg gtttgggcgt tacagctgga gtagataata
     7801 aaacgcggtg ccatcgttga gagataatca cacagcggtt taccaatgcc gcggcgcggc
     7861 gggttaacca gcaccagctc cggcacctcc ccctgagcag tggcaaactg agtggagtcc
     7921 agcgcctgaa attgcaaacg cgttaagccc agttcagcgg ctgactgctt tgcacaggca
     7981 atggcctctg gcgcaatttc gatcccggtt aactgcatgt caggcatcgc gcagtgtaaa
     8041 ccaaagcccc ccacaccgca gaacagatcc cacatatgtt taaccgacag ctgtcgtacc
     8101 cagtcgcgcg cggtggcgta caactggctg gcgaccgccg gattagtctg gaagaaactt
     8161 tgcggacgga tccacagcgg tacgtcatta aaacgctctg ccagcgcctg ttgttcagtc
     8221 aggtaaatct ccgtctcccc ttccataatc gccatatgta ccggctgaat attgacggta
     8281 ataactttca gctgcggtag ttgttcctgt aaccacggca gcgccttacg cagttgcgcc
     8341 agcttggtat cagaacgcag tacaaagcgc agcatcatgc cgccatcgct ctggctttca
     8401 gtcagcagaa tgtatttcag ttcgccacgc ttacgcgcca cgttgtaggg cgttaacccc
     8461 gctcgggcga taaacggttt tagcgccgca aaaacgggcg caaatgaggc aggataaagc
     8521 ggacagtcac aaaggtcttc cggcgtgccg tcgcgatgca gcataccgag cagtggtttt
     8581 tcaacgctac cactcaccac cattttggct ttattacgaa acccttgttc cgggcctgac
     8641 accggcgcgc accattcctc aaccggaaag tcggcgagca gatttttaag atcggcggtt
     8701 ttagcggaga gttgctctgg aatcggctgc gttatccact gacaggaacg acagcgaccc
     8761 gcgtcgtaaa gtgcgcactg catacattga ccttcacatc atcaggggcg acgattatac
     8821 acattattgc aactggaaga accgccgact ggtcgaggga acgaacagca gcatcaggat
     8881 gagcatatcc ggcagcttct gcagcatcag gctatggaag atttcacgtt ttgattcacc
     8941 gggaatgctg aacagctccg gataaccata ccccagcgaa gctgcccaca agtaacttgc
     9001 ggccgtgatt tgtgtcaaca gatacagcca gcgcgcccag cgacgccctt tcaccagtga
     9061 aaacgcacac cagatctcaa tgaaaaccag caccagactg ctcaaaaaga ccagcgttaa
     9121 actccaggtt tgtacgctgc gatgaatgaa ctcgccaata ccgcgcacgc ccagcgtatt
     9181 gaaaatcatc agcacgtcga ggccacggat cataataatg gcgagcgccg ccacctgcac
     9241 cagcgcaggc acattcaggc gagcatgaga tgaagatgtt ttcttaaaaa atcccaacgt
     9301 ttcgtcttcc atgaaaacaa tgccgcgaca tgcgcggcat tatgtagcca ggttggcaaa
     9361 ttttagtgtc ttcagccacg tcttgcacgc tggatgtcgc gtatccgctg tttttctgcg
     9421 cgcgccatca gataccaggc gataaatccg acaattccga ccgcgccgag gatcaatgtt
     9481 gccagggcgt tgatttccgg attcaccccc atccgcacgc tggaaaagac cagcatcggt
     9541 aaagtggtgg ctcccggccc ggaaacaaag ctggcgatca ccagatcatc aagcgacaaa
     9601 gtaaaagcca gtaaccagcc agaaatgatc gcgggcatga tcatcggtag cgtaatgaca
     9661 aaaaacactt tcagcggcgt cgcaccgaga tccatcgcgg cttcttctat cgagcgatcc
     9721 agttcccgca gacgcgacga aataacgact gccacataag ccgtacagaa agtgacatgc
     9781 gccagccaga tggtgagcat accgcggtcc gcaggccagc caatagcatg agcaagcgcg
     9841 acgaataaca acaatagcga caagcccgtg atgacatctg gcatcaccag cggcgcggtg
     9901 atcataaagg caaaaccatt tgatccgcga aacctaccaa agcgcaccag cacgaccgcc
     9961 gcaatcgtcc cgaggatcgc cgccgccgtc gccgcacagg ccgcaattgt caggcttaaa
    10021 ccaaccgcac tcatcatcgc gttatcgcgc aataactcac cataccagcg tgttgaccag
    10081 ccggcccaca ccgtcaccag tttcgagctg ttgaatgaat agatcaccag catcagcatt
    10141 ggcgcgtaga gaaaggtgaa gcccagcagc aaaatcacaa tccgccaggg cgaacgaaca
    10201 accggtaaat tattcatccg tgttctccca cgcttttttg ctggtgtttg tgaaaccaca
    10261 ttatcggcac aattagcagc agcaacatga tgatcgctac cgccgaggcc accggccagt
    10321 cgcggttatt gaaaaactcc tgccacagca cgcgcccgat catgatgctg tccgggccac
    10381 cgagcagttc agggatcaca aactcgccca cagccgggat aaacaccagc atcgatccgg
    10441 caataatccc gcctttggtc agcggcacga tcacagtaaa gaacgttttc agcggtcgtg
    10501 caccgagatc cagtgccgct tccaccagcg aataatcaat acgaatcaag gcggtgtaga
    10561 tcggcagcac cataaacggc acataggcgt aaacaatgcc gatataaacc gccaggttgg
    10621 tatgcagaat ggtcaacggt tgatcgataa cccccagcca cagcagaaaa ttattcagca
    10681 caccgttgtt ttttaatatt cccatccagg cataaacgcg gatcagaaac gaggtccacg
    10741 acggcaggat caccagtagt aataagatat tacgggtcga aggcttactg tgcgccaccg
    10801 cccacgccag cggatagccg atcagtaaac agcaaaatgt cgaaatcgca gccacctgta
    10861 gcgactggag ataagcatcg aaatagagcg gatcgtcagt cagttgcaga aaattaccga
    10921 gattaagagt gatggacagt tgcccgtcag cccactccat cagttcggta taaggcggaa
    10981 tggcgcgcgc catctccgcc agacttattt taaagacgat cagaaatggc agcagaaaca
    11041 gcaggatcaa ccagatatac ggcaacgcaa tgaccagttt gcgcccatgc ttcatttgca
    11101 gctgcgacaa ccacagctta aatccgcccg gcggtttggg ctgggcagca ggttcaagtg
    11161 tactcatctt tgctccttaa accgtcagca ccacacagct gtccacttcc cagcacaaac
    11221 gcacttcgtc gccccaggtt ggtaaccctt tgcgatatcg atgagcgttt tgtagctgag
    11281 cgctgatcat ctgtccgctc ttgagacgaa cgtgatacac cgaaagatcg ccgagatagg
    11341 caatgtgcat cacctcacct accgcaaagt taaaaccgtc agcgggaggt tcctcgcaaa
    11401 gcatgatttt ttccgggcgc agcgccacat gcaccggcac attatcgacc accgaagcat
    11461 ctacatcgac tttcagtgga tgcaccagcc ccggcgaata aagcaccaga ccatcttcct
    11521 gacgctcttt gagtacacct tcaaagacat taaccgagcc aataaattcg gcgctgtagc
    11581 gagtggtcgg atgctcgtag atctcttccg gttcgccaat ctggacaaat ttccctcgat
    11641 tcataatggc gatacgcccc gccatggtca tcgcctcttc ctgatcgtgg gtgaccatca
    11701 cacaagtcac accgacacgc tccagaatat ccaccacttc aagctgcatc cggtcacgca
    11761 gctttttatc cagcgcgccc ataggctcat cgagcagtaa tagtttcggg cgcttggcaa
    11821 ggcttcgggc cagtgccaca cgctgtcgct gaccgccgga aagctgatgc ggtttgcgtt
    11881 tggcgaactc ctgcatatgc accagcccga gcatctcatt gacccggctg gcaatttccg
    11941 ctttcggtag tttgtcctgt ttcaggccaa aagcgatgtt ctgttccacg gtcatatgcg
    12001 gaaacagcgc gtaagactga aacatcatat ttatggggcg caggtaaggc ggaacctgtg
    12061 acaaatcgac gccatcaagc attatctgtc cggcagaagg ttgttcgaaa cctgccagca
    12121 tacgcagcag cgtggacttg ccacagccgg atgcgcccag cagcgcgaag atttcgcctt
    12181 tatagatggt caggctgaca tcatcgaccg catgctgacc atcgtaagat ttagtcagat
    12241 tacggatttc taatagcggc gtcagcacct tacgggtttt cgcctgcggg cgagggatag
    12301 cgtcattcac ggctgctctc cggcattgaa tgtgtgcaag cgcaccataa tggtgcgaat
    12361 tgccggcggg cgtggtgcgc ccgtccggca tctacgactg cggattattt tccgctcttc
    12421 actttggtcc acgcgcgggt gcgcacacgg tcgattttcg gatcctgtac tttcagagtg
    12481 aacagcttcg cacgaacatc cgcaggcggg taaatacccg ggttatcacg gacttccgca
    12541 ctcaccagcg gcgtagctgc tttattggcg ttagcataga acacatggtc ggaaatatgc
    12601 gccaccacat ccgggcgcag caggtaattc aggaactgat aggcttcgtc tttatttttg
    12661 gcatccgcag gcatggcgaa gacatcaaag aacgccatcg ccccttcttt cggaatcgag
    12721 aacgagacat taacgccatt cttcgcttcc ttcgcgcggt ttgacgcctg ccagacatcg
    12781 cccgcccagc caatagcgac gcagatatcg ccgtttgcca ggtcgttaat gtattgagat
    12841 gaatggaaat aacgaatgtt cgggcgcagc tttaacagca gatcggttgc cggtccggtg
    12901 taatcatctg ctttagtgct gttgggatct ttaccgagat aattcagcac ggtagcaaac
    12961 acttcttccg gtgcatcaag gaaagagacg ccgcagcttt tcagtttttc cagattttca
    13021 ggcttgagga tcaagtccca gctatcgacg ggcgcgtttt cgcccagcac cgctttaact
    13081 ttatcaacgt tatagccaat cccggtcgtc gcccacatat agggcatagc aaatttattg
    13141 tcgggatcgt gtttggcaac taacttcagc agctccggat cgagattctt ccactccggc
    13201 aatttgcttt tgtccagcgg ctggaaaact cccgcagtca actggcgttc aagaaagctt
    13261 gctgacggca ccaccaggtc aaagccggtg ctacccgcca ttaatttgcc ttccagtact
    13321 tcgttagagt cgaaaacatc gtagacgact ttaataccgg tttctttttc aaaattggcc
    13381 accgtgtccg gggcgatata atcagaccag ttataaatgt gaagtgtttt ttgttcagcc
    13441 gcgagcgtgc cgacagagac ggccatcaga gcacccgtaa ccagacccga tagccatttt
    13501 ttatttaagg cggtcatatc tctttccttc tgaaagttcg ttaacaaacg aacaaaagtt
    13561 gtcacactga aagatatgca ataaacgtgc atatttttta tcggcgcggt gtaagaaaag
    13621 agaactctct cccggccagc actgctcgct atataaaagc atcgcaagaa taactttagc
    13681 cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt tatgcgtatt
    13741 tgctctatgt gataaagcca aatcggcaca agagaagtga aataatcttt aagaaaaggt
    13801 taagaatagc agataatttt gcagcacgca gtcactgcgg cagcgactgc gtactcgaaa
    13861 ggagttatca gtggaggaaa tagttttcgg taacgttgtt ttgcccttca tcgtcagtcg
    13921 gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc tcttcgctct
    13981 gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat aaagactggc
    14041 aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc acggtcagcg
    14101 aactggcatt gatggcagat aaatccgccg ccagcggcaa tacggctgaa ggtcggactt
    14161 ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca tcaaagacac
    14221 cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttatcacat tcaaaaaaac
    14281 tcatccccag gtcatcgagc cattgacgca gcgtatccag accaggaacg accagcgatg
    14341 tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc aaaaagcgca
    14401 ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg caatattccg
    14461 tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatgtcgata ccggtggttt
    14521 tttctattcc ttccagcccc ggcgacgcat tcacctccat caccaatggc ccgcgattag
    14581 cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct tttatcgcga
    14641 tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga tgcaaattgg
    14701 aacgaaaatc gccctctttc gcccgccgtt caatcgcagc gaccacttca tcgccaacaa
    14761 ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc agaatatgcg
    14821 cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc ctggcgcgtc tccgccagca
    14881 cgacaccaat tccctgcgtg ccttcaacca gcttgaccac cagcggcgca ccaccgacca
    14941 tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca ggcaggtcga
    15001 tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg gcaatggcga
    15061 ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc gccgtcccat
    15121 aaaaggtaat ggcggtacca atacgcggga tcacagcatc aaaatggggt aacttgcggc
    15181 ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa agcggatcaa
    15241 gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta cacgaataga
    15301 gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc agacctggtc
    15361 aaaagcccgc gtatcataca tcggcgtggc ggttttagcg cgtcgcccaa ccctgtttgt
    15421 gcaaataatc cagtataaat gggcggcttt ctttaatgat tgttcggcgg atatgatcgc
    15481 tccaggtatc ccggcgatta ttgctgccac gggtgaggta atattccgcc agttgttcgt
    15541 catactgcgc cagtgcgtct ttatccagcg gttgatagtt gttttcatgc accaaaatgg
    15601 aggccggtaa acgcggctta agatccggat tatccgcagg ccagccaagg cacagcccaa
    15661 acagcggcag aacatgctgc ggtaatttca gcagctccgt caccgcttca atattattgc
    15721 gcaggccgcc gatatatacc ccgcccaatc ccagcgattc cgctgcggtt aatgcattct
    15781 gcgccatcat tgccgtatca acgacaccga gcaacagttg ttccgccagg ccaagttgtg
    15841 catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag aactccgccg
    15901 cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ttcatcacgt aacgctttgt
    15961 cggtaatgcg aataatgcta ctgcactgca aaaaactgga actggacgtc gcacgggcgc
    16021 tgttaataat cgcctcacgc tgcgcttcgg aaatgggttc atcagtgaaa tggcgaatgg
    16081 agcgatggcc acaaataagt tcaatggttg gcgtcattat ctttttctct ttctgaacgt
    16141 gaatattgcg gtggacggtt catcagctgt ggggcaagac gttttgccac ctgaagaata
    16201 accaccaccg cagcgggaag catgagcaaa acaccgagaa aaatcatcag aatctgcact
    16261 tctggccgag aaaatggctc aggcagcgac agggagccgc ttaccgacag cagcgccacc
    16321 gccagcaaca tcattccgat aaattccagt atcaacacgc ttttaggcaa tttaccgatc
    16381 gcgcgcatac gcttccctct gcaaagtgag ccttcagtct aaaacttttc actgtattgt
    16441 gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt tcagcgaata
    16501 cattttagcg tgatcattac aggtataaat ctatgaggag agaaataatg caaaccgtta
    16561 tttttggtcg tccgggttgc ccttactgtg tgcgtgcaaa agatctggct gagaaattga
    16621 gcaatgaacg cgatgatttc cagtatcagt atgtagatat tcgtgcggaa gggatcacta
    16681 aagaagatct acaacaaaag gcaggaaaac ccgtagaaac cgtgccgcag atttttgtcg
    16741 atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagag aatctggacg
    16801 cctgatcgtc tgacaagccc tcgcgttgag ggctttattg attttttctg tgctgtggtt
    16861 taaacaaact actgataaat aagaaacaca atgcccccag cgcacaccag aacaccgcac
    16921 ttaataacca tgccagctct tgccagaatg agcgcgtcgg tgaaaaaaac agccgcataa
    16981 tgagcatcga acagggtgcc gccagcattg cgccaaacag aggtttcagg acttctctac
    17041 gatgtgaaaa gaagctggca actgcaccag gaagaatgaa aaatagcaaa ccgatttcag
    17101 gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc cagaaaaaga cacaccacaa
    17161 tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc actcgttcct
    17221 cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta agataaagcc
    17281 gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgttga tacctaatgt
    17341 gattacacta gtaaaatata ttgttacttt actatcgttt aggtgcgctg aatgaatctg
    17401 cgccctgaat tctggtaaaa aacattatcg taaattacca tttctttcaa cagcttacta
    17461 gtaaacaaga agttagcctc cgtgaatata aacgtcgccg aattgttaaa tgggaattac
    17521 attctgttat tatttgtggt cctcgcgctt gggctatgtc tcgggaaatt acgacttggt
    17581 tcgatccaac tgggtaattc cattggcgtt ttagtcgtat cgctgttatt aggccaacaa
    17641 catttcagca ttaacaccga cgcgcttaat cttggcttta tgctgtttat tttctgcgtc
    17701 ggggtcgaag ccggaccgaa ctttttttcc attttttttc gcgatgggaa aaattaccta
    17761 atgttagcac tggtgatggt tggcagtgcg ctggtcatcg ccttagggtt aggtaagctg
    17821 tttggctggg atattggcct gacggccggt atgttagcag gctctatgac gtcaacaccg
    17881 gttctggtcg gtgctggcga tacactgcgt cattccggca tggaaagcag gcagctctca
    17941 ttggcactgg ataatctgag cctcgggtat gccttaacct atttaatcgg cctggtgagt
    18001 ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc aggacttaca gaccagcgcc
    18061 cagcaaatcg cccgcgaacg tggcctggac acagatgcca accgtaaggt ttatttgccg
    18121 gtgatccgcg cctaccgcgt cggcccggag ctggtagcct ggaccgacgg caaaaatctg
    18181 cgtgaactgg gtatttatcg acaaactggc tgttacattg aacgtattcg gcgtaacggg
    18241 attctggcaa atccagacgg tgatgccgta ctacaaatgg gcgatgaaat agcgttggta
    18301 ggctatcctg acgcccacgc ccgactcgat cccagtttcc gtaatggcaa agaagttttc
    18361 gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag tggtcgttaa aaaccataac
    18421 gctgttggca aacgcctggc acaactgaag ttgaccgatc acggttgctt ccttaaccgc
    18481 gtcatacgta gccagattga gatgccgatt gatgacaacg tcgtgcttaa caaaggtgac
    18541 gttttacaag tcagcggcga tgcccgccgg gtaaaaacca tcgccgatcg catcggcttt
    18601 atctcgattc acagccaggt cactgacttg ctggcattct gcgccttctt tgttattggg
    18661 ctgatgatcg ggatgatcac cttccagttc agcacattca gtttcggtat ggggaacgct
    18721 gccgggttgt tattcgccgg aattatgctg ggctttatgc gtgctaacca cccgaccttc
    18781 ggttacattc cgcagggtgc attaagcatg gtgaaggagt tcggcttgat ggtgtttatg
    18841 gcaggcgttg gtctgagcgc tggtagcggt attaataacg gcctgggcgc gattggcggt
    18901 cagatgttga ttgccggatt gattgtcagt ctggtgccag tggttatctg cttcttgttc
    18961 ggtgcttatg tattgcgaat gaaccgcgca ctgttgttcg gcgcaatgat gggcgcacgc
    19021 acctgcgcgc cggcaatgga gatcatcagt gatacagctc gcagtaatat cccggcactg
    19081 ggctatgcgg gcacttacgc aatcgccaac gtcctgctga cgctggcagg gacaatcatc
    19141 gtcatggtat ggccaggatt aggataaaac tgaagttgcc ctgaaaatga aatttttttt
    19201 gcacaaccgc agaacttttc cgcagggcat cagtcttaat tagtgccact gcttttcttt
    19261 gatgtcccca ttttgtggag cccatcaacc ccgccatttc ggttcaaggt tgatgggttt
    19321 tttgttgtct aaaatttatc tactaccctg caaccctctc aaccatcctc aaaatctcct
    19381 cgcgcgatag cggcttacgg tcggtgacaa aatgcagcgt catcccctca ataaacgcat
    19441 caagcgcgcg ggcggttccg ggttcaaacc attgttcgag cgtttgctga ctgcgctgca
    19501 tccagttttg cattaccgtt tttaatagcg gttttcggct agccagcgcg tagagctggt
    19561 acatcagctc catgttatcc ggcgttgcaa cctgtgagct gtaaatcata tcggtgatag
    19621 cctggcatgc gcccggagca tcactaacat cgctaaaaaa tgcctgatat tgccgggaca
    19681 tgatctcagt aaaactgctg aacgcctcca gcaacaactc atcaattcct gaaaaatagt
    19741 aggtcatcga ccccaacggt accccggcaa gggtagcaat tttgcggtgc gtaacagcat
    19801 gtattccata aagtttcacc gcctccagcg tggcctggat aattttttct cgccgttgcg
    19861 gatcgttagc gcgacgcatg tcatttcctc tctctgcaat tgtgtacaaa tgtacacaac
    19921 cttgctaatg ttgtcttccc tcttcttatt ttgacgtgat ctatgaccgt aaattcttca
    19981 cgtaatgcat tgaaacgccg aacctgggcg ctgtttatgt tcttcttttt gccaggcctg
    20041 ttaatggcgt cctgggcaac ccgtacgcct gctatccgcg atattctttc tgtttcgatc
    20101 gctgaaatgg gcggagtcct ctttggtctg tcgatcggtt caatgagcgg tattctctgc
    20161 tcggcgtggt tagtgaaacg ctttgggacg cgtaatgtca tcctggtcac gatgtcctgc
    20221 gcattgatcg ggatgatgat actaagtctg gcactctggc tgacatcgcc cctactcttc
    20281 gcggttggtc tcggcgtctt tggggcaagt tttggttctg cggaagtggc gataaacgtt
    20341 gaaggtgctg ccgttgagcg agaaatgaat aagacggttt tgccgatgat gcacggtttt
    20401 tatagcctgg gcacgctggc aggcgctggt gtcgggatgg cactaacggc ctttggcgtt
    20461 ccggcaacgg tgcacatttt attggcggcg ctggtaggca tcgcgcctat ttatatcgcc
    20521 attcaggcaa tccctgatgg tacgggcaaa aatgctgccg atggcaccca gcatggtgaa
    20581 aaaggcgtac ctttttatcg cgatatccag ttgctgctga ttggtgttgt ggtgctggcg
    20641 atggcctttg ccgaaggttc tgccaacgac tggttaccct tattaatggt tgacggtcac
    20701 ggttttagcc ctacttccgg ctcgctgatt tatgccggtt ttaccctggg gatgaccgtt
    20761 ggacgcttta ccggcggttg gttcattgac cgttacagtc gcgttgccgt ggttcgggcc
    20821 agtgcactaa tgggggcgtt gggtattggg ctgattattt ttgtcgatag cgcctgggtt
    20881 gctggggtgt ctgttgtact ttggggactg ggtgcctcgt tgggcttccc gctgaccatt
    20941 tctgccgcca gcgataccgg ccccgatgca ccaacccgcg tcagcgtggt agctacgacc
    21001 ggttatctgg ctttcctcgt cgggccgcca ctgctgggct atctcggcga acattatgga
    21061 ttacgtagtg caatgctggt tgtactggcg ctggttattc tcgcggctat tgtcgcgaaa
    21121 gccgtcgcca aacccgatac caaaacgcag acggcgatgg agaatagttg atgagcatta
    21181 aattaattgc ggtagacatg gatggtactt tcttaagcga tcaaaaaacc tataaccgtg
    21241 agcggtttat ggctcagtat cagcaaatga aagcacaagg cattcgcttt gtggtcgcca
    21301 gcgggaatca atattatcag ttgatctctt tctttccgga aattgctaat gaaatagcct
    21361 ttgtggctga aaacggcggc tgggtagtga gcgaaggcaa agatgttttt aatggcgagc
    21421 tgtcgaagga tgcgttttct actgtcgtgg aacatttgct gacgcgcccg gaagtggaaa
    21481 ttattgcctg cggaaaaaat agcgcctata cactcaaaaa atatgacgat gccatgaaaa
    21541 cggtggcgga aatgtattat caccgtctgg aatacgtcga taactttgac aacttagaag
    21601 atatcttctt taagtttggt ctgaatcttt ccgatgaact gattccacaa gtacaaaaag
    21661 cattacatga ggccatcggc gatattatgg tgccggtcca caccggcaac ggcagcatcg
    21721 atctgattat ccccggcgta cataaagcca atggccttcg ccaactgcag aaattatggg
    21781 gaatagacga cagcgaagtg gtggtctttg gcgatggcgg taacgatatt gagatgctgc
    21841 gtcaggcagg ctttagtttt gcaatggaaa atgccggcag cgcggtcgtc gcagcggcaa
    21901 aataccgggc aggctccaat aaccgtgaag gcgtactgga tgtgatcgat aaagtgctta
    21961 aacacgaagc gccatttaac caataaaacg gcagcacgcc cggcaacaca atacctgaat
    22021 gaaggctgca taattcgcgg aaattaaccc gctgcattat ttcaggcgaa atgattatga
    22081 aaaattgtct actgttgggc gcgcttttaa tgggctttac tggtgtggcg atggcgcaaa
    22141 gtgtcaccgt cgatgtgccg agtggataca aagtggttgt ggttcctgat tcagtcagcg
    22201 ttccacaagc ggtcagcgtc gcgactgtgc cgcaaaccgt ctatgtggct cctgcgccag
    22261 ctccggctta ccgcccgcat ccttatgttc gccatcttgc cagcgtaggt gaagggatgg
    22321 ttatcgaaca ccagattgac gaccatcacc attaattcga gaatgcctga tcgcacaaag
    22381 caatcaggca tttttttacc cttcgtgcga atttcccatc tgtttatctt ttaaaaagat
    22441 aaccatcagc aacagccaca aaattccgtt gacaaggttg aagagattaa acagtccgtt
    22501 gcccccgttc agccagacat gtttgctgat ttcaataccg acggtaaaga tcagcatttg
    22561 cagcatcccc atcgccgcag aaaccgtacc tttactcata tcgctggcaa acagggttaa
    22621 tcgcaccagt cccgcattcg ccagaccaat accgaaagca taaatactta acccggcggt
    22681 catccataaa tacgcgtgcg atgagataac cgttgccgca gcagcgacca acagaccaat
    22741 cataatcggc cagccgccca taataatcag cgaacgtacg gtgcggcgcg aggtcagacg
    22801 cgctaacagc aagttacccg caattaacgc cccgaaaata ggcacttgca gcaagccata
    22861 ttcatagctg ctcaactgct caccggtaat aatgataatc ggcgactggg cgatccacgc
    22921 caacaacggc aggctaacga atcccagcgc cagcgccccc gccacaaagc ggccgttctt
    22981 cagcaccagc ttatagtcac gaccgagttc tttcagcgac agtttctcgc ctatacgcgt
    23041 ggcggtttca ggcatggctc gttgcagacc gaaaaaggag atcgctgcca atgcggcaaa
    23101 caagacaaac atcccttccc agggcagcac atggatccac gccgcgccca ccagcggacc
    23161 aagtagtgga gcaatcagcg ccacgttcgc cattagcgcg gtgatcttga tacaaaccgc
    23221 ctcttcgaag gattcctgaa ttgcggcgta tcccacagcg ccaatgaaac agaggcttat
    23281 gccctgcaag aagcgcaaca gggtgaattg ttcaatgttt tgcgccagta atattgccag
    23341 acaggtgacg ataaaccaca ccactcccgc cagcatcacc ggacggcggc caatacgatc
    23401 cgacaacggc cccaggagcc attgtaaaaa catcccgccc gccagatacg cagtcatcga
    23461 agtaggaacc caatcaatgc ccgcctgata ttgttccacc acggccaaca taccgggttg
    23521 aatcatatcg ttgccgatat aggttgaaaa ttcgtaaagc accagacaga gagggaaaag
    23581 taacgcctga cgtccaagcc tggcaccgga agctaattta ttttgcatgc aatttcttcg
    23641 ccaataataa tcgcgcaaag tttaataaaa gcgcagctaa cgagaaagcg aattttgtag
    23701 ctgaaaccac ggttaagcac attcttacat tattgcgagt atagctacgc tttctttaag
    23761 ttttatttaa cctttgcccg ttacaatcac ccaccgtaaa caggccgctt gagggaaata
    23821 agacgatgcc gctttaccca gtttaacctg cactttattc tcaacgactt gcctgtattg
    23881 gctccctttt aatcactttg cgtcgggaag ttatgctgga aaatttgaat ctctctctat
    23941 tctctcttat taacgcgacg ccagactctg cgccgtggat gatctcgttg gcgattttta
    24001 ttgcaaaaga tttgattacc gtggtgccgt tgctggccgc ggtactttgg ctgtgggggc
    24061 ttacagcaca acggcaactg gtgataaaaa tcgctatcgc gctggcggtc agcctgtttg
    24121 tgtcctggtc gatggggcat ctttttccgc acgaccgacc ctttgtcgaa aatatcggct
    24181 ataacttcct gcatcatgcg gcggatgact cattcccgag cgatcacggt acggtgattt
    24241 tcacctttgc actggcattt ttatgctggc atcgcctgtg gtccggctca cttttaatgg
    24301 tgctggccgt cgtcattgcc tggtcgcgcg tttatcttgg cgtccactgg ccgctggata
    24361 tgctcggtgg attgctggca ggtatgattg gctgccttag cgcccagatt atctggcaag
    24421 cgatggggca taaactctat caacgtctgc aatcgtggta tcgcttctgt tttgcattac
    24481 cgatccgcaa aggctgggtg cgtgactgat ctctgctaaa aagtgtagta ttgagcggct
    24541 cgcttcaata actattcaga gggattatgg aaacacgtcg cgaagagcgt atcgggcagc
    24601 tgctgcaaga attaaaacgc agtgataagt tacatcttaa agacgccgcc gccctgcttg
    24661 gggtttcgga gatgacgatt cgtcgcgatc tgaacaacca cagtgcgccc gtcgttttgc
    24721 tcggcggcta tattgttctg gaaccacgca gcgccagcca ttacctgtta agcgatcaaa
    24781 aatcccgcct ggtggaagaa aaacgccggg cggcaaaact ggctgcgacg ctggtagaac
    24841 ccgatcagac cctctttttt gactgtggca ccaccacgcc gtggattatt gaagcaattg
    24901 ataatgaaat cccttttacc gccgtttgtt attcgctaaa tacctttctg gcgctgaaag
    24961 agaaacccca ttgccgcgcg tttctttgcg gtggtgaatt tcacgccagc aacgccattt
    25021 tcaaacccat cgattttcag caaacgctga ataatttttg cccggatatc gctttttatt
    25081 ccgctgcggg cgtgcatgtc agtaaaggcg ctacctgctt taatcttgaa gagttgccgg
    25141 taaaacactg ggccatgtcg atggcgcaaa agcatgtgct ggttgtcgac cacagtaaat
    25201 ttggcaaggt gcgtccggcg cgcatgggtg acctgaaacg ctttgatatt gttgtgagcg
    25261 attgttgccc ggaagatgag tatgtgaagt acgcgcagac gcagcgtatt aagttgatgt
    25321 attaatgacg tataaccgga tgacgtttcg cgccatccgg ttatcagaag attaagagaa
    25381 ccagctgccg aaccactgat ggaatttcat catcacgaaa tcccacatcc gaccaaagaa
    25441 tccgccctct tccacatttt ccatcacgat cagcggacgc tgctcaatgg atttaccgtt
    25501 aagctggaaa tcaatggtgc cgacaacctg accttttttc agcggtgcgg taagctgcgg
    25561 ttccgttaac gtataactcg ccttcaggtt tttcagctgc ccacgcggta tggtcactga
    25621 gcccgcttcg cctgccccga gattcacttc gctcttatca ccaaaccaga cgcgctgagt
    25681 cacaaatgtg gcatcaggtt taattggcgt cacggtttca aagaaacgga agccccaggt
    25741 caataatttc tcagactcat taaaacggat acggtcagtt ttcgccccca gcactacgga
    25801 gattaaacgc atatcgccct gggtagccga agcaaccaga ttatatcccg cgcctgccgt
    25861 ggttcctgtc ttcatgccat caacattcag attgctgctc cacagcagac gattacggtt
    25921 aggctgacga attttgttga aggtgaactc tttctcttta tgaatggcgt actcttccgg
    25981 cacatcgtgg atcaatgctt tacccagcaa tgccatatcg cgcgcggtac tgaactgccc
    26041 cggcgcatcc agaccgtgca ccgtctggaa ggtagtgttg gtcagaccca gttttttggc
    26101 ataaccattc atcagaccaa taaatgactc ctggctcccg gcaacgtaat cagccagcgc
    26161 aatacaggcg tcattaccgg actggataat cacacctttg ttcaagtctg ccaccgaaac
    26221 ctgatcgccc ggtttgagga acattaccga tgaaccacgc agtgccggat ttcccgtcgc
    26281 ccaggcatct ttaccgaccg tcaccatatc ggtgagttta atcttatcgg ccttaagcgc
    26341 ctgcccaacc acatagctgg tcatgatttt agtcaggctc gcggggtcca gtttttcatc
    26401 cgcattgcct tctgccagca ctttaccgct ggcgtaatcc attaaaatcc atgcacgcgc
    26461 atccacgctc ggcgcttcaa cggtttgttc cgccgcgaat gccgttgggg caaaaaggaa
    26521 taaaaatgca gaacccgctg caagaccacg aaggagagag gagtattgcg tcataaatgc
    26581 cacccgagta tccattcaaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta
    26641 ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa
    26701 tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa
    26761 ctttacttca ccatcggagt cctgttcagc ctgtggctgc ctgtcggaaa aggggtgagt
    26821 atgattacgc tgtggggtcg gaataattca accaacgtaa aaaaagtatt gctgacgctc
    26881 gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac
    26941 gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa
    27001 agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg
    27061 caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagccgaaaa atggatggac
    27121 tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca
    27181 ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg
    27241 tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctcaggcga cgagtttggt
    27301 gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg
    27361 acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc
    27421 aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg
    27481 gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc
    27541 tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc
    27601 acaatgacat ctttatcttt cagcgcgcca ataaataatt tttgctgcca ctgggggaat
    27661 ttgtcgctgt tatagaaggc catgccgctc acggcgggcg aatctttcca gtaaaaaaca
    27721 ggttgctcgg tcccggcgac gatctcccct ttcgcctccg gtatcttaaa gcctgaatag
    27781 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
    27841 tcatcgccac cgcgcgggcc atgttcattc agccatagtg cattactcca cggattcatc
    27901 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgtgc atcggattcc
    27961 tttataaaag gattatcatc cgggatttct cccaggtcgg tcagtcgcac cagtttgccc
    28021 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
    28081 agataacctt taccgtcaaa taccatccgc ccgccaaaat ggttgccggt agacagtttt
    28141 ggcatctgac gaaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
    28201 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
    28261 atgcggcgcg actgagcaaa atcgggcgct aaaaccacgt ccagcaggcc gccctgcccg
    28321 tgcgcccaaa cgtccggcac tccggaaagc ggcgcagata atccttttcc tgcttgccag
    28381 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattgtcggg taaaaaggcc
    28441 agcgcccagg gatggtcgag tttgtcctgc agtacttcga catttaccgt tgcaggagcc
    28501 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
    28561 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaatttattt
    28621 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
    28681 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
    28741 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
    28801 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
    28861 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
    28921 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
    28981 cgtatgcggc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
    29041 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
    29101 tattttttga gcaaaatgct gtctaggatc aattcttcac gccgaattaa caaaatttac
    29161 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
    29221 gaatccctgc cctgaccttt actgtaactg aatatttttt aagctatgag caaagtaact
    29281 ccccagccga aaatcggctt tgtttccctt ggctgcccga aaaaccttgt cgattcagag
    29341 cgtattctca ccgaactacg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
    29401 gacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
    29461 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
    29521 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
    29581 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
    29641 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
    29701 atttctgaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
    29761 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt tgatgcgggc
    29821 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgtcga tgttaaacat
    29881 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
    29941 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
    30001 gacgtcatcc cactgatggc agaaggcaaa atcctgccct atctggacat tccgttgcag
    30061 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactc
    30121 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
    30181 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
    30241 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
    30301 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
    30361 ttgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
    30421 attatcgacg aagtggacga agaaggtgcg attggtcgca gcatggcaga tgcaccggaa
    30481 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
    30541 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaatctta
    30601 aggcagatct aacaaggata ttgttcttcg atctgcctct gctacccttc ccgatgtcct
    30661 acccttacgc acaaaatccc ctgacgcata aatcgagaaa aatgaatcgt actcgaggca
    30721 actctcactt tttcacacca aattaaagtt ttgatcgcaa ttacttgaaa taagcgtaat
    30781 ggttactata gtaaacatta ccacaaggat ggatgtatgg gtgtgtataa ggcaagacga
    30841 ttcagccaaa gcacgaaaaa acttggtatt cacgataaag ttctcatggc tgccgctgaa
    30901 gaagtgattc agggcatatg ggaggctgat ttaggtagcg gtgtcatcaa aaaacgttta
    30961 cccctccaac aaggaaagag tggtggagcc agaacaatta tcttttttaa atcagcaaat
    31021 catgtgtttt tttatgatgg ttggtccaag agtggtctaa gcagtaaagg aaccaaagaa
    31081 atcgaagatg atgaactggc ggcctacaaa aaaatggcaa atgcatttct ggcctttagc
    31141 aataagcaaa tagaggattt gatcgaaact ggctttttga ttgaggtgaa aaatgagcga
    31201 taaattacaa atgatgctgg aagatgcaaa ggaactgaat gcactgggcc tgatgtctga
    31261 tagtgaagtt gatgccattg ccgtcatggt caaagctcgt gaagtcagcg cccgtatagc
    31321 caaagtcaaa tcaatgacgg gtgaagagat caaagccatc cgcacccgat acggcatgtc
    31381 acaatccgtg ctggcacata caatgggaat gtcaaaagaa agtgtctcga aatgggaacg
    31441 taatgaaata aaaccgagcg gcccggcatt acgtattttg aatacattgg caataaaagg
    31501 gccggaagtt tttgcaaagt aaaatgtttg caaatgattt accacattca ttagtacccc
    31561 ttgattaagg ggtactaaca ctattctaat tctactgcca gctaatctgc gccctgccgt
    31621 ttttcttact cttatacagt cgctcatccg ccagcatttt caagcgttcg acggtagtac
    31681 attcatttga atgcgctaca ccgatactgg tgcttaaatt aatgcttccc acctggaaat
    31741 gaacaatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
    31801 caaattcgtc accgccgaag cggtagactt tacctttatc tacggggagc tgtgatttaa
    31861 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
    31921 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
    31981 ttaaatcttc gtcaaaggat tttctgccca tcgctttggt cagatcgtcc aggaatgccg
    32041 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
    32101 gtacgcgttc aaaatggtta ttaataatgg cttctttctc cacaatacca cgattaatca
    32161 ctctgcgtac cagatagagg caactggcac ccgatatcac tgccaccaat gcggcaaaga
    32221 aaataatcaa tgtacatacc tgaaaataaa cgtctttttt ataagcaaat acgtacaacc
    32281 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac agtgaagatg gcgtttttag
    32341 cgttatattc atcaccggtc accgatttac cgttcttgaa cagggagaac atcaggttct
    32401 cgctatccat cgccagcgat tttgacagta aagaaaaacg ttccagacca agcaaaaata
    32461 tcagactacg attgtgagtt accggataga ggtaatagac gaaggtttta ccatcaaaag
    32521 agataaaccc tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat
    32581 aatttttctc gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga
    32641 catcgtacaa ataagagggt gaaaccgagt cggcgtaagc tgtgcgtaag ccaaccaaca
    32701 gctgttcctc cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt
    32761 cagcaatatt tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc
    32821 gttcctttac catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca
    32881 gcagactgac tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact
    32941 aattttccac ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga
    33001 gcctgaacac atcctaattt gattaattcg cgcgcaacgt cttcgctctc tacgccttcc
    33061 gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc
    33121 gcagtaccaa aaatatcgcc ggtaaactgc gagtccacct ttatgctgtc tggattaatt
    33181 tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc
    33241 ccttgtttac gcagttgttc aatattgaga taaaaatgtt cattaattcg ctcaatcacg
    33301 gtttcagtga tctcaacaca aaggctgttc gccttcaggc cattttcttt catgatggta
    33361 gtaaaacgct catgaaagtg tggttcgttt aactgccagg gcgaaacatt aatatgcagt
    33421 aagaaatcat ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc catcgcggtt
    33481 ttaataattt gatagcccag ctcattgata aacccgatat cttcagcaat ggtaatgaac
    33541 ttcagcggtg aaatgatccc ccgctccgca gacacccaac ggcacaacgc ctccgcgccc
    33601 agaatggcgc gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt
    33661 tccattgcat ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc
    33721 gcatcatcac agaaaataaa ctctgagaac atatggttgc ggatactgga aatcgccagt
    33781 ttcagctttt ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatagcagac
    33841 ttatggatgc gcagattgcg gtagccaaaa ctgctgtcaa aatcgtttac cgtcgctatc
    33901 ttacgggtaa acgtctgtac attttcccgg cacaggagta aatagagatc ggcattaaat
    33961 gtcacgatat cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg
    34021 attttattgc gttcatcaac accaaacacc gcttccacag cttccagacc acacagttag
    34081 atgatcccca cagccagata ctgattattg ttggattcaa taatcgccat atttttaata
    34141 aaaccgcttt cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg
    34201 gcatctttca tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg
    34261 cgaattttca gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc
    34321 agctcggtca tttcagaaaa ttgccgacgg aacacacgag tggtcattaa ataaaccacc
    34381 aacaatacga taataatgaa cgcaaacggc agataaaaaa gtttatctga tgcgttcaat
    34441 aaattaccac caggataata aaaatagatt tcgaataatg cgttgtttgg caccgctgat
    34501 gttttttcaa tatattccg
//
LOCUS       DAZGTJ010000049        33906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000049 DAZGTJ010000000
VERSION     DAZGTJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.049"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(47..955)
                     /gene="galU"
                     /locus_tag="ACHK8E_002878"
     CDS             complement(47..955)
                     /gene="galU"
                     /locus_tag="ACHK8E_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP0345117.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1157..2170)
                     /gene="rssB"
                     /locus_tag="ACHK8E_002879"
     CDS             complement(1157..2170)
                     /gene="rssB"
                     /locus_tag="ACHK8E_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP0345118.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2262..3167)
                     /gene="rssA"
                     /locus_tag="ACHK8E_002880"
     CDS             complement(2262..3167)
                     /gene="rssA"
                     /locus_tag="ACHK8E_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP0345119.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3280..3738
                     /gene="ychJ"
                     /locus_tag="ACHK8E_002881"
     CDS             3280..3738
                     /gene="ychJ"
                     /locus_tag="ACHK8E_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP0345120.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3788..4630
                     /gene="purU"
                     /locus_tag="ACHK8E_002882"
     CDS             3788..4630
                     /gene="purU"
                     /locus_tag="ACHK8E_002882"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP0345121.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4790..4874
                     /locus_tag="ACHK8E_002883"
     tRNA            4790..4874
                     /locus_tag="ACHK8E_002883"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4824..4826,aa:Tyr,seq:gta)
     gene            4921..5052
                     /locus_tag="ACHK8E_002884"
     ncRNA           4921..5052
                     /ncRNA_class="other"
                     /locus_tag="ACHK8E_002884"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5237..5914)
                     /gene="narI"
                     /locus_tag="ACHK8E_002885"
     CDS             complement(5237..5914)
                     /gene="narI"
                     /locus_tag="ACHK8E_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0345122.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5914..6624)
                     /gene="narJ"
                     /locus_tag="ACHK8E_002886"
     CDS             complement(5914..6624)
                     /gene="narJ"
                     /locus_tag="ACHK8E_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0345123.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6621..8159)
                     /gene="narH"
                     /locus_tag="ACHK8E_002887"
     CDS             complement(6621..8159)
                     /gene="narH"
                     /locus_tag="ACHK8E_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0345124.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8156..11899)
                     /gene="narG"
                     /locus_tag="ACHK8E_002888"
     CDS             complement(8156..11899)
                     /gene="narG"
                     /locus_tag="ACHK8E_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP0345125.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(12292..13683)
                     /gene="narK"
                     /locus_tag="ACHK8E_002889"
     CDS             complement(12292..13683)
                     /gene="narK"
                     /locus_tag="ACHK8E_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP0345126.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            14022..15818
                     /gene="narX"
                     /locus_tag="ACHK8E_002890"
     CDS             14022..15818
                     /gene="narX"
                     /locus_tag="ACHK8E_002890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP0345127.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            15811..16461
                     /gene="narL"
                     /locus_tag="ACHK8E_002891"
     CDS             15811..16461
                     /gene="narL"
                     /locus_tag="ACHK8E_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP0345128.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(16462..17856)
                     /gene="ychO"
                     /locus_tag="ACHK8E_002892"
                     /gene_synonym="iatA"
     CDS             complement(16462..17856)
                     /gene="ychO"
                     /locus_tag="ACHK8E_002892"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP0345129.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            18042..18395
                     /gene="ychN"
                     /locus_tag="ACHK8E_002893"
     CDS             18042..18395
                     /gene="ychN"
                     /locus_tag="ACHK8E_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP0345130.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(18439..19134)
                     /gene="chaC"
                     /locus_tag="ACHK8E_002894"
     CDS             complement(18439..19134)
                     /gene="chaC"
                     /locus_tag="ACHK8E_002894"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP0345131.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(19292..19522)
                     /gene="chaB"
                     /locus_tag="ACHK8E_002895"
     CDS             complement(19292..19522)
                     /gene="chaB"
                     /locus_tag="ACHK8E_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP0345132.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            19792..20892
                     /gene="chaA"
                     /locus_tag="ACHK8E_002896"
     CDS             19792..20892
                     /gene="chaA"
                     /locus_tag="ACHK8E_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP0345133.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            21297..21404
                     /locus_tag="ACHK8E_002897"
     CDS             21297..21404
                     /locus_tag="ACHK8E_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0345134.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(21553..22407)
                     /gene="kdsA"
                     /locus_tag="ACHK8E_002898"
     CDS             complement(21553..22407)
                     /gene="kdsA"
                     /locus_tag="ACHK8E_002898"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP0345135.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(22443..23252)
                     /gene="sirB1"
                     /locus_tag="ACHK8E_002899"
     CDS             complement(22443..23252)
                     /gene="sirB1"
                     /locus_tag="ACHK8E_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP0345136.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(23256..23648)
                     /gene="sirB2"
                     /locus_tag="ACHK8E_002900"
     CDS             complement(23256..23648)
                     /gene="sirB2"
                     /locus_tag="ACHK8E_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP0345137.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(23645..24478)
                     /gene="prmC"
                     /locus_tag="ACHK8E_002901"
     CDS             complement(23645..24478)
                     /gene="prmC"
                     /locus_tag="ACHK8E_002901"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0345138.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(24478..25560)
                     /gene="prfA"
                     /locus_tag="ACHK8E_002902"
     CDS             complement(24478..25560)
                     /gene="prfA"
                     /locus_tag="ACHK8E_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP0345139.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(25602..26858)
                     /gene="hemA"
                     /locus_tag="ACHK8E_002903"
     CDS             complement(25602..26858)
                     /gene="hemA"
                     /locus_tag="ACHK8E_002903"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP0345140.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            27072..27695
                     /gene="lolB"
                     /locus_tag="ACHK8E_002904"
     CDS             27072..27695
                     /gene="lolB"
                     /locus_tag="ACHK8E_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP0345141.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            27695..28546
                     /gene="ispE"
                     /locus_tag="ACHK8E_002905"
     CDS             27695..28546
                     /gene="ispE"
                     /locus_tag="ACHK8E_002905"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP0345142.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            28697..29644
                     /gene="prs"
                     /locus_tag="ACHK8E_002906"
     CDS             28697..29644
                     /gene="prs"
                     /locus_tag="ACHK8E_002906"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP0345143.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            29769..31448
                     /gene="dauA"
                     /locus_tag="ACHK8E_002907"
     CDS             29769..31448
                     /gene="dauA"
                     /locus_tag="ACHK8E_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP0345144.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(31503..31781)
                     /gene="ychH"
                     /locus_tag="ACHK8E_002908"
     CDS             complement(31503..31781)
                     /gene="ychH"
                     /locus_tag="ACHK8E_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP0345145.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            32059..32643
                     /gene="pth"
                     /locus_tag="ACHK8E_002909"
     CDS             32059..32643
                     /gene="pth"
                     /locus_tag="ACHK8E_002909"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP0345146.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            32760..33851
                     /gene="ychF"
                     /locus_tag="ACHK8E_002910"
     CDS             32760..33851
                     /gene="ychF"
                     /locus_tag="ACHK8E_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP0345147.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         8214 a         8966 c         8420 g         8306 t
ORIGIN      
        1 gcgtcgattg ctcaacgccg tttcgtggat aacaccgata cggatgttac ttcttaatgc
       61 ccatctcttc ttcaagccag gctttaaatt ccgtaccaag ggtgttatga cgaataccgt
      121 attcaacgaa ggcctgcatg taacctaatt tattaccaca gtcatggctc ttccctttca
      181 tatgataggc ttccaccgtt tctttttcga tcagcatatc aattgcgtcg gtgagctgaa
      241 tttcatcacc agctcccgga ggggtttttg ccagcaacgg ccaaatatcc gcgctaagta
      301 cgtaacgacc cacaatagcg agattagacg gcgcaacatc cgcttttggt ttttcaacca
      361 caccaaccat cggtacgctt tcacccggcg ctaattcaac gcctttgcaa tccacaacgc
      421 catatgcggt aacatcagca accggttcaa ccatgatctg gctatgaccc gtttcatcaa
      481 agcggcggat catctctgcc aggttatcct gtgacaaatc ggattcatat tcatccagaa
      541 taacgtcagg caaaataaca gccacaggtt catcacccac taccgggtga gcacacaata
      601 ccgcatgtcc caggcctttc gccagcccct gacgaacttg cataatagtc acgtgcggcg
      661 gacaaataga ctgcacttca tcaagcagtt gacgttttac acgtttttcc agcattgctt
      721 ccagttcaaa actggtatca aagtggtttt caatagagtt tttagatgag tgagtaacca
      781 gcacaatttc agtaatgcca gccgcaatac attcattcac gacgtattga attaatggct
      841 tatcgacaag tggcagcatc tctttcggga tggctttcgt cgccggcaac atcctggttc
      901 ctaatcccgc aacggggata acggcttttt tgactttcgt attaatggca gccatttaaa
      961 ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc tgtatcgcat cccagtatat
     1021 cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt tagcataatt aagacaatta
     1081 cttatacgaa tggcgacgat agtaccacca gccgacatta gcaggtaatg caaatttagc
     1141 ccgcgttatc gtttgctcat tctgcagaca acatcaagcg aagccgacca ccggttcccc
     1201 atatttggca ttgccaggca tcgcatcgct ggctcaattg attcaaataa gcgttaccta
     1261 aagtgcctaa cggaacacca ttactgattt gcacctggtg ttcgccggta tttaacgtcg
     1321 cattcagacc cgcagaaacc agaatgagat ttttcagttc gcgatgataa tagccaacta
     1381 atagcggaaa ctgccccggc agattggcct gacggagtaa atggtttacc tgcttcaata
     1441 acgcgcccaa ctctggcaaa cgttgatttt ggtgtgcaag ctgttcctgt aataatccgt
     1501 taaacaatgc gcgtaataac aaggcagcaa gtacgccatt atgtccagct cgggtgacat
     1561 caaggcagta aaatgccaga tcgttttccg aaagtgcggc aatatcaagc accaggccgg
     1621 gtttgtccgc ggcaaccaat tgacgataat taacccggca atgggaaatc acctgctgaa
     1681 ccggcggttg caattcctgt aataatttcg ccgctgcggc agggttatca accattgcat
     1741 cccagtcgcg aaaaagcctt tcctcttcct caacgcggga attaaacatg ctgggataga
     1801 gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt aactggtttc agcaaaacat
     1861 cttcaacgcc cagacgtaac gctttggcaa tatctgccat attttcagtg gcagatatca
     1921 ccagaactgg ggtctggtcg cctctgttac gtatatgctc cagcagttta agcccgttca
     1981 ttcgtggcat cgcgatatca catatcatca ggtctggagt gaaacctccc agcaactcaa
     2041 gggcatccac cccatcagcc gccagtaccg ttgtcgctcc caatgaggaa aaccacgaat
     2101 ccagaagcga gcgaaatacc tgctcatctt caacaatgag aatctgtttt ccgaccaatg
     2161 gctgcgtcat gttctctccc ctgactggct ttactcaata gtggcatgct attgccacct
     2221 gcgcctgtca gaattaactt aagtgtagat aaaaattctg gtcaaatgtt ggtgcgtacc
     2281 aacggcaaaa gttcgtccat tttcttttcc actgccagct gcccggccgc aatggcagcg
     2341 tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa caggttgaat cagaatatcg
     2401 ggcggatcac ctgccatgcg gttcctttta aggcggttct ccagcacctg gatagaagtg
     2461 gtcataatct ctgttgccgt tggcgctgtc accgcccgac gtgtcgttat gctgcctaac
     2521 ctttctttca gacgcgcatg ccacggcaga gaatcaccat tctcgctatt ttcttcactg
     2581 acattaaagg agagcaaatc ttgttgcatc aaatgagcat cgtgctgcag gtcgaccgct
     2641 atcacaatat cagcccccaa tgcacgcgtg agggaaatag gaattgggtt aactacagcg
     2701 ccatcaacca gccagtagcc gttatgcgca acgggtgcca ttagtcctgg aatactgcat
     2761 gatgcacgaa tagcaagatg gagatcgcct tcagtaaacc ataattcgcg cccagtactt
     2821 aaattggtgg caacagccgc gaagcgacgg gaacaatttt cgatctctgt ttccggcatt
     2881 atttcgcgat attgattgaa gacacgctcg ccgcgcagta acccaccgcg ctgccaggag
     2941 agatccatca ggcgtaaaac atcccaataa ctgaaagagg tcacccaatc ttccagcgca
     3001 gatagtcgat cgcatgcata ggcagcgccc accagcgaac caattgaaca tcctgcaacg
     3061 atatcaattt caatacccac tttttttagc gcattaataa cgccaatatg cgaccaacct
     3121 ctcgccgcgc cagatcccag cgccagccct atttttatct ttctcattat tcccgcaaga
     3181 ttcccctgga atgcaatcgt agcaacatcg ctactgctcg gttaacatag tgctactcag
     3241 gcggttaata cgccgttatt gtttccaggg agattatttg tgtctcagct ttgtccctgt
     3301 ggtagtgctg tcgagtatag cctatgttgc cacccttatg tgtctggtga aaaggttgca
     3361 cctgatccag aacatctcat gcgttcgcgt tactgcgctt ttgtgatgaa agacgcagat
     3421 tatttaataa agacctggca tccatcttgt ggagcggcga cgttacgtgc cgaattgatt
     3481 gccggatttg cgcatactga atggttggga ttgaccgtat ttgaacattg ttggcaggac
     3541 gtcgacaata tcggatttgt cagttttgtt gcgcgattta ccgaaggtag caaaacaggt
     3601 gcaataattg aacgttcgcg atttttgaaa gaaaacggtc aatggtacta tattgacggt
     3661 acacgtccgc agtttggccg caacgatccc tgcccttgtg gttcaggtaa aaaatttaaa
     3721 aagtgctgcg gccaataatg gttgacggta cggtttagca aacactctca acaaggtttt
     3781 tccagcaatg cattcactcc aacgtaaagt tctgcgtact atttgtccgg accaaaaagg
     3841 tctgatcgca cgtattacca atatttgcta caagcacgag ttaaatatcg tacagaacaa
     3901 tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc acggaactgg aaggaatttt
     3961 taatgattcc accctgctgg cggatctcga cagcgcattg ccagaaggct ccgtacgtga
     4021 gctgaatcct gccggtcgtc gccggatagt gattctggtc actaaagagg cgcattgcct
     4081 tggcgatttg ttgatgaaag ccaattacgg cggcctggat gtcgaaatcg cggcagttat
     4141 tggtaaccac gatactttac gttctctggt tgagcgtttt gatattccgt ttgagctggt
     4201 aagccatgaa gggttaagcc gcaacgagca cgatcaaaag atggcggatg ccattgatgc
     4261 ttatcagcct gactacgtgg tgctggcgaa gtatatgcgg gtattaacgc cagaatttgt
     4321 ggcacgcttc ccgaataaga tcatcaatat tcaccattca ttcctgccag cgtttatcgg
     4381 cgcacgtcct tatcaccagg cctatgaacg tggcgtgaag attattggcg ctaccgctca
     4441 ctatgtgaat gacaatctgg acgaaggtcc aatcatcatg caggacgtta ttcatgtcga
     4501 tcatacctac acagctgaag atatgatgcg cgcaggtcgt gacgtcgaga aaaacgtctt
     4561 aagtcgcgca ctctacaaag tactggcgca gcgcgtcttt gtttacggta atcggacgat
     4621 tattctttaa tcgccagcaa aaataactgg ttacctttaa tccgttacgg gtgaaaatta
     4681 cgcaaccagt tcatttttct caacgtaaca ctttacagcg gcgcgtcatt tgatatgatg
     4741 cgccccgctt cccgataagg gagcaggcta gtaaaaagca ttaccccgtg gtggggttcc
     4801 cgagcggcca aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc
     4861 ttcccccacc accatcactt tcaaaagtcc gtgaactctc aaacgaatcc gcgattaaat
     4921 attctgccca agcggggaag gatgagaagc ttcgaccagg gttcgactcg agcgccagcg
     4981 agagagcgtg gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg agtaatcctt
     5041 cctccaccac catcacttcc tgtaaatact caaaacctgt tactccgagc taattctgcc
     5101 caatggcacc gcgtttaccg gacctacaat atcctcccct gtaggtcgga taagcgaagc
     5161 gcatccgaca actcaccccg taccgtgccc ataaaaaaac cccgccgaag cggggtctat
     5221 gcaaactaaa attcgcttag tgacgagcgc gcaccagctg atacttacgt gtcagatact
     5281 ccaccggtac gctccagatg tgcaccagac gcgagaacgg gaacagcagg aataacgtca
     5341 tccccaacac caggtgcaga cggaagataa acgccacacc atcaaggtgt tgagaagcgc
     5401 caccgtggaa ggtcactacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt
     5461 ccatatgttg ggcggagaac ggaatggtca acaggcccag cgcgcactgg ataacgagca
     5521 gcgacaggat caggatatcc gctccggtag tggttgcacg cacgcgtggg ctgaacagac
     5581 gacgcttcag caacagcacg ccgccaatca gacacagcac accgctggca ccaccagcaa
     5641 acatcgccat tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg
     5701 tcagcatacc gaagaagtga ccaacaaaaa tccccagaat accgatatgg aacaggttcg
     5761 acgccaggtt catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact
     5821 gcccgtagtc ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt
     5881 agatatcaaa gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt
     5941 cagatattgc ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga ttgctgaatc
     6001 accgcagcct ttttcagcaa agaatttaac ctgctcttct tcccagactg catccagcgc
     6061 ctgcggcgta tcgtcgcgcg cttcatcggc aattttttcc gccactttgt cgctgtcgat
     6121 ggcggtattc gccagtttca gcagcagatc aaacagcacg gcataacggc tttcacgctg
     6181 ttgcagacgc gcgctcagca atgccagaat cggcgcaata tctttcaaac cctccacggc
     6241 ttcgctttgc ggcagctgcg ccaggtactc cagatacagc ggcagatggt cgggcaattc
     6301 gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc agcagatcca ccatcgcctg
     6361 gccgcggtca cgggattcac cgtgaacatg ttcaaatagc aacagtgacg tggcgcggcc
     6421 acggtcgaac agttcgctgt actgcgcctg ggcatcgagc ggatccatcg tcgttaaatc
     6481 gcgcaggaaa atgcccagcg catgggcatc ctcttttggc agatttttcg acgcggcaat
     6541 cgcctcaaac atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg
     6601 cgatacaatc acgagttcga tcatgggtgc ggctccgttt tactggtcac gtcgatggca
     6661 tcgatacgac ggctgttaaa cagattgaat ttggtatctg agccgtggca gccatcacca
     6721 aaggtaaagc cgcagccatt tttctccggg aaggcttccc gcgccagttc acgatgactg
     6781 ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt
     6841 gcctgggctt cggtcagacc gacctcttcc agcgcacggg tatcaacttt accgtcaacg
     6901 gtttcagcac gtttataatg acgcatcgcc agcatacgtt tcagcgcgcg cagtaccggt
     6961 ttggtatcac ctgcggtcag cagattcgcc agatactgta ccggaatacg caggctttcg
     7021 acgtctggca gaatgccatt gctgcccagc tcacccgcat ctgccgcaga ctgaatcggc
     7081 gacagaggtg gcacatacca gaccatcggc agcgtgcgat attccggatg cagcggcagc
     7141 gccagtttcc attccatcgc cattttataa accggcgact gctgtgcggc ttcaataacg
     7201 ctcagcggaa taccgtcttt aatcgcctgc tcgatgacct tcggatcgtt cggatcgagg
     7261 aacacgtcca gctgacgctg gtagagatct ttctcgttct cggtgctggc tgcacgttca
     7321 atcgcgtcgg catcgtacag cagcacgcca agataacgga tacgaccgac acaggtttca
     7381 gagcacaccg tcggctgacc ggcttcaata cgcggatagc agaagatgca cttctcagac
     7441 ttaccgctct tccagttgaa gtagattttt ttgtacgggc atccggtgat gcacatacgc
     7501 cagccacggc atttgtcctg gtcgatcagg acgatgccat cttcttcacg cttgtaaatc
     7561 gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca caggcgtggc
     7621 aaatacatca tgaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag
     7681 ttcttgtctt tcgccagttt gtcaaactcg ccgcccaggt catcttccca gtttggccct
     7741 ttttcgattt tcgccatccg ttcgccggta atcagcgaac gtggacgggc aatcggctgc
     7801 gatttgctgc cttccggcgc ggtatgcagg ttctgatagt caaaatcgaa cggctcgtaa
     7861 taatcgtcga tccccggcag atgcgggtta gcgaaaattt tacccagcag catggcgcgg
     7921 ttacccatgc gcggttgcag tttgccgttg attttacgga tccagccgcc tttgtatttt
     7981 tcctggtttt cccagtctgt cgggaagccc tggcccggct tggtttccac gttgttgaac
     8041 cacgcgtatt ccacgccttc acggctggtc cagacgtttt tacaggtcac tgaacaggtg
     8101 tggcaaccga tgcacttatc cagattcagc accatgccga cttgtgaacg aattttcatt
     8161 ttacgctctc ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct
     8221 tacgcactac aacaaattca tcgcggttag aacctacagt gccgtagtag ttaaagccgt
     8281 atgccagatg ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg
     8341 agttatgaat accaccgcgc tggctggtga tttctgaacc cggcaggtta acgatacgtt
     8401 cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg
     8461 cagtcaatgc accgttgctg ttgaagactt caatccagtc gttgtcggcg atacccagct
     8521 ctttggcatc ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca
     8581 gcaggttgtc gctgtaggtg gagtggatac cccacttctg gtgcggtgtc aggaagttga
     8641 gcgctttttc cggattaccg ttagatttct ggcccatcac ttctttcacc gaacgggtgt
     8701 cgatcggcgg acgataaacc agcaggcttt caccgaaatc acgcatccac tgatgatcct
     8761 gatacagctg ctgacggcca gagagcgtac gccacgggat cagctcgtga acgttggtgt
     8821 aaccggcgtt gtaagaaaca tgttcatctt caaggccaga ccaggtcggg ctggagatga
     8881 ttttgcgcgg ctgcgcctga atgtcacgga agcggatctt ctcgtcttct ttattcagcg
     8941 ccagatgcgt atggtcacga ccggtaaact cactcaatgc cgcccaggct ttcacggcca
     9001 cctggccgtt ggtttctggt gccagcgtca ggatcatctc tgctgcatca attgcggtgt
     9061 tgagcatcgg ctggcctttc gccggacctt ccgctttggt gtagttgagc ttacgcagca
     9121 gatccatctc gctctgggtg ttccaggcaa tccctttacc gccgttaccg attttctcca
     9181 tcagcgggcc gatagaggta aagcgttcgt aagttgccgg ataatcgcgc tctacaacca
     9241 taatgtgcgg tgcggtttta cctgggatca ggtcacattc gccttttttc cagtctttca
     9301 catccagcgc ttgcgccagt tcagcggcag agtcgtgctg gataggcagc gtgacgatgt
     9361 cggtttcttt gcccagatga ccaacgcaca cttcggagaa tttcttcgcg atggctttgt
     9421 agatttccca gtcgcttttc gcttcccagg ccggatcgac cgcagcagac agcgggtgaa
     9481 taaacggatg catatccgaa gtattcatgt cgtctttttc gtaccaggtc gccgtcggca
     9541 aaatgatgtc ggaatagaga caggtgctcg acagacggaa gtccagcgtc accaccagat
     9601 ccaacttgcc ttccagaccg ttgtcctgcc agtccacttc ttccggcttc acgccgccct
     9661 gctgacccag gtctttaccc tggataccgt gctccgtccc cagcaggtac ttgagcataa
     9721 actcatgacc tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttacgcg
     9781 ggtggttttt accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg
     9841 atttcaccgt atagtccacc ggattcatcc cggctttttc tgcttcgcga gcgatagtca
     9901 gcgggttagt gcctaactgc ggcgcagacg gcagccagcc catacgttct gcacggacgt
     9961 taaagtcgat cagatgtccg gtataacggg atttatccgc catcggcgac aacaactctt
    10021 ctgcggtcac ggtttcataa cgccactggc tggagtggtt atagaaataa gaggtgctgt
    10081 tcatgtgacg cgccggacgc tgccagtcaa gagcaaacgc cagcggctgc cagccggttt
    10141 gcggacgcag tttttcctga cctacatagt gcgcccagcc accaccgctc tgacctacac
    10201 agccgcagaa aatcagcatg ttgatcagac cacgatagtt catatcgagg tgataccagt
    10261 ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg cgtcttgtca gcgttatcgg
    10321 caaattcacg ggcaatgcgg ataatctggc tgcgagaaac gccagtgatc tgctcggccc
    10381 atgcaggggt gtaggctttc acgtcgtcat agctggttgc gcagttaacg tcgttcaggc
    10441 cacgttccag accgtagttt gccaacgtca gatcataaac ggtggtcacc agagcagtgc
    10501 tgccatcagc cagttgcagg cgtttcaccg gcagcttgtg cagcagcacg ttttccagtt
    10561 ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta tgggaagccc acttcagcga
    10621 tctcatcctg gctacccatc aggctcagtt gcagctcggt ttcttcgcca gttttgccgt
    10681 cgcgctgctc aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg
    10741 gcgcaaccat ttctccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct
    10801 ggcccagcgc atccaccaga tcagcagcgc gcagcatacg acccgcagcg tagtaaccgt
    10861 cgcgctcttc cagcatcacc agcattggca tgtcggtata acgacgcaca tagtcggtga
    10921 aatactggct tgggttgtcg aggtggaatt cacgcagcat cacgtgaccc atcgccagcg
    10981 ccattgctgc gtcggtgccc tgcttcggtg ccagccacag atcgcacagt ttggcgattt
    11041 cagcgtagtc cggcgtgacg gcaacggttt tggtgccttt gtaacgcact tcagtaaaga
    11101 agtgagcatc cggagtacgc gtctgcggca cgtttgaccc ccaggcgatg atgtagctgg
    11161 agttgtacca gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgtggagacg
    11221 caggaggcag atcgcagtac cagtcgtaga agcttaagca agtaccgcca agcagcgaga
    11281 gataacgcgc acccgacgcg taagaaacca tcgacattgc cggaattggc gagaagccag
    11341 cgacacggtc cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt
    11401 tcacttcctg ccaggaagaa cgaacaaaac caccgcgtcc acgcgcctgc ttaaagcttt
    11461 tcgctttatc ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg
    11521 ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcagacggt
    11581 tggcgctgta aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt
    11641 ttggcagatc cgggcgagta cgcggatagt cagtctgctg ggtttcccag gtgaccagac
    11701 cgtttttaac gtagattttc cagctgcagg agccggtgca gtttacccca tgggtagagc
    11761 ggacgatttt gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat
    11821 tgagaagctg gccatgccca tcggcaaagg tttcgccctt ctgtttgaag tagcgaaacc
    11881 ggtccaggaa tttactcatc ggttttctcc tgtgggagcc tgtcggctct ctgataaatc
    11941 gacattgcta atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga
    12001 tcaagagtga tggggatgaa aaataaagta aattcctaag atacccctta aggataatta
    12061 ataaattcaa cataaatcac tgtaaattaa gatattttgc atccatagtt atttagacta
    12121 ttgagatttt tatgcagatg ggtattaagg agtattcccc atgggtaaca tattaatggc
    12181 tcttaatgaa aaccggcgaa atacgccaca cttcataagg atgttgtaac taaatgacag
    12241 gtaaaaaaag cgcgacatca tgccgcgcaa aggataatca aatattaaga tttatttttt
    12301 agaatgccga ccatataccg cccaggtaat cactacgcag gcgatataga aaatcaaaaa
    12361 tactttcatt gcgccgactg gcgaacccgt taatgccagc gagctaccga acgctttcgg
    12421 gataaagaag ccaccaatcg cgccaatcgc agagatgaaa cccagcgccg ccgccgtgtc
    12481 ggttgccgct tcacgcatcg cacgttcgtc agagccccct tctgctttca cgcgatccat
    12541 tgtcagttta cggaagatca cggaaatcat ctgaaaagtg gaaccactac ccagcccagc
    12601 tgtcaggaac agcgccagga agactgcgaa gaacgccatg aagcttccgc cctgtccgtc
    12661 agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc atcaggataa agttcaccag
    12721 tgtgacacga gttccgccca gacggtcaga taatgcacca cctgcagaac gcgccagcgc
    12781 accaataaac ggcccgaaga aagcgtattg cagaatctga acatccggga actgcgtttt
    12841 tgacagcatc gcaaagcccg cggagaagcc gatgaaggag ccgaaggttg ccagatacag
    12901 caggctcata atccacagat gaccccgttt gagtaccggc aactgctcct tgatggaggc
    12961 tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga tggcaaggaa
    13021 cggcacccat acccaggacg cattcgccag atacagctca gtcccatccg gctgtttgac
    13081 accctggcta ccaaatactg cgaaaatcga cagtgatacc accagcggag caaccaactg
    13141 catgacgctg acgcccatgt tgcccagacc accattcaga cccagcgcgc caccctgctt
    13201 ctgtttcgga aagaagaagc tgatgtttgc catactggat gcgaagttcg cgccagcaaa
    13261 gccgcacagc agagagatga tgatgaagac gctataaggc gtggaggtat cctgcacggc
    13321 aaaacccagc cagacgcaag gaataatcag aataccagtg ctgaacgccg tccagcgacg
    13381 accaccgaag ataggaacca taaaggagta tggaacacgt aataacgcgc cagaaaccga
    13441 aggcagcgcc gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga ctttcggtaa
    13501 gttcaccgca acagcgctga acaacatcca tacgcaaaac gccagcagca gacagggaac
    13561 ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga acgcaggatc
    13621 ttccggtcgc caatctgtaa tgacagctcc agtagccctt tcgggggcgg atgagtgact
    13681 catagacacc tctgatactc gtttcgatgt ctgccacctt agtgtctgta gctaaaggca
    13741 atttgatgta aatcaaacga taagataact ttatctatca ttgatattta tcattaccca
    13801 tagtgagtac agtgatttca taaaaattat gagatttttc acggtgctgt aaaatcccta
    13861 cccttaccga tgtaaagcga ctaaccacgc ggcaaataag gagtaactct ttccgggtat
    13921 gggtatactt cagccaatag ccgagaatac tgccattcca gaatgtatcg tcacattcat
    13981 taaggttatt gctcatttaa agcctgaagg aagaggtttg catgcttaaa cgttgtctct
    14041 ctccgctcac cctggttaat caggttgcgc ttattgtgtt gctttctact gctattggac
    14101 tggcagggat ggcagtttct ggctggctgg tgcaaggcgt tcagggcagc gcccatgcga
    14161 tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct gttggcagca gtgccattaa
    14221 gcgagaaaga caagccctta attaaagaga tggaacaaac agcatttagc gccgagttaa
    14281 cccgagcagc agaacgagac ggacaactgg cgcaattaca gggtttacaa gattactggc
    14341 gtaatgaact gatccctgcg ctaatgcgtg cacaaaaccg cgaaacggtg tcagcggatg
    14401 tcagccagtt tgttgccggg cttgatcaac tggtatctgg ttttgaccgc accacggaaa
    14461 tgcgcattga gacagtggtg ctggtccatc gggtaatggc ggtatttatg gcacttttac
    14521 tggtgttcac tattatctgg ttgcgggcgc gactgctaca accgtggcgg cagttgttgg
    14581 caatggcgag tgccgtcagc catcgcgatt ttacccaacg cgcgaacatc agcgggcgca
    14641 acgaaatggc tatgcttgga actgcgttga acaatatgtc tgcagaactg gcggaaagtt
    14701 acgccgtact tgagcagcgg gttcaggaga aaaccgccgg gctggaacat aaaaatcaga
    14761 tcctctcttt tttatggcag gccaaccgcc gtttgcattc ccgcgccccg ctgtgtgaac
    14821 gcctgtcacc tgtactcaac ggcttacaga atttaaccct gctacgtgat atcgaactgc
    14881 gggtgtatga cactgatgat gaagagaatc atcaggagtt tacctgccag ccagatatga
    14941 cctgcgatga taaaggctgt cagctctgcc cgcgcggtgt actccccgtt ggcgatcgcg
    15001 gcacaaccct gaagtggcgg ctggctgact ctcatacgca gtacggtatt ttgctggcaa
    15061 ccctgccgca ggggcgtcat cttagtcatg atcaacaaca actggtggat accctggttg
    15121 aacaactcac cgccacgctt gcgctggatc gccatcagga acgtcagcaa cagttgatcg
    15181 tgatggaaga gcgtgccacc attgcgcgcg aactgcatga ttctattgcc caatctctct
    15241 cttgcatgaa gatgcaggtg agttgtttac agatgcaggg cgatgcgctg ccagaaagca
    15301 gccgcgaact gttaagtcag atccgtaacg aactgaatgc atcctgggcg cagttgcgtg
    15361 aattgctcac cacattccgc ttgcagctca ccgagcctgg attacgtccg gcgctggagg
    15421 cgagttgcga agagtacagc gccaggtttg gcttcccggt gaagctggat tatcaattgc
    15481 cgcctcgtct ggtgccttcg catcaggcaa tccatctgtt gcaaattgcc cgtgaggcat
    15541 taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt ggtgacagtg gcgcaaaacg
    15601 ataatcaggt caaactgacc gtccaggata acggctgcgg cgtgcctgaa aatgccatcc
    15661 gcagcaatca ctacggcatg ataataatgc gcgaccgtgc gcaaagttta cggggcgatt
    15721 gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt attcccgaaa
    15781 aaactttcac agacgtccaa ggagataccc atgagtaatc aggaaccggc tactatcctg
    15841 ctgattgacg atcacccgat gctgcgaact ggcgtaaaac agcttatcag tatggcacca
    15901 gatatcaccg tggttggcga agcgagtaat ggcgaacagg gtattgaact ggcggagtct
    15961 cttgatcccg atctgatcct gttagatctc aatatgcccg gcatgaacgg tctggaaacg
    16021 ctggataaac tgcgcgaaaa gtccctctcc gggcgcattg tggtattcag cgtctctaac
    16081 catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg atggctatct gttaaaagat
    16141 atggaaccgg aagatctgct gaaagcgtta catcaggcag ctgctggcga aatggtatta
    16201 agcgaagcat taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc cactactgag
    16261 cgcgatgtta accagttaac cccacgcgag cgcgatattc tcaagctaat tgcccagggt
    16321 ttgccgaaca agatgattgc ccgccgcctg gacatcaccg aaagcacagt aaaagttcac
    16381 gtcaagcaca tgctgaagaa aatgaagcta aagtctcgcg tggaagctgc ggtatgggtg
    16441 catcaggagc gcattttctg attacggttc ccagcgcagt tcgtcgtttg acaatgcgtc
    16501 gaacggttcg acaagcgtta gcgtgatctc attggaggag acacgctgcc cctggttatc
    16561 ttccaccacc actgacaatc gccagtgatt gcttgcgcct tccccgtttt gccagtcagg
    16621 catgatcagc gtccagccct cctcactgtt ggcttgtgcg cccggcgtca aacttaatat
    16681 ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa cgactgcgga tttgtaattt
    16741 cagcggcact gtttcgccag gttttagatc ccacggcggt gtcgccagaa acaccgttaa
    16801 cgtttttcgc tgtcggtact caagagtcgg tagattattt cgctgcggat tgtcatagcg
    16861 actaccacgt aacgactgac tttcggcaac ctcgcccgca gaaagttgtt ttttgagcgg
    16921 tacaccaaag cggtaattaa gattcagccc gaggttattt tgattttcgc cactttcacc
    16981 ctgtttatgc tgggccgtca cagtgactaa tggcactggg gtataattta atcccagact
    17041 caacgcgacg ggattgtgat aacccgtacc agagttaaac aaatcaacac gatcaccaaa
    17101 atactgttct acgctgacac tggtattgag gtgttgataa aacggcatgc gcatccgggc
    17161 tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt catgccatgc
    17221 agcaaacggc tgataaaagt ttgccgatag tcgcaaatat tcgccccacg cttcggcacc
    17281 gaagcccgct cgctgaagat tttcgtccag caagttgtca taaaaagtgt tataacccac
    17341 cagccagctg ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca gcccatcatc
    17401 ctgctgagta agaccaagct ggctccaggt gagataacga tcattatctt gtaacggcac
    17461 aaaccagctt ccacgactgc cggtgaaatg tccttcgtta tccactttga cgtcaacact
    17521 ggcatttccc cacgctgata gccacgactc tacgtgctga ttaacctgtt gactaaacgc
    17581 gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca gcccgttatc
    17641 attcatactg gtttcgccga aatctttcac aatttcagca aagtgttttt ccccatcatg
    17701 attttcaggt gccatgccta aatccggcag accatcattg ttattatcaa agggatttgc
    17761 cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg cctgccgcca gcaagagtaa
    17821 ataaaacgga ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt ccggtaaaaa
    17881 atgaaaaacc atagccctaa agaataacga ttactggcaa cttttgtcgt attttctcgc
    17941 gctttcagga aaatccttaa aaatgtgacc gattggtcag acgctgcaga agcagtaaga
    18001 tagaagatga gtgtctaatt ccctgaatac aggagtaaat aatgcaaaaa atcgtgatcg
    18061 ttgccaatgg cgcaccttac gggagcgaat ccttatttaa cagcttgcgg ctggccattg
    18121 cgttacgaga gcaggagagc aatctggatc tgcgtctgtt cctgatgtct gatgcggtca
    18181 cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa cattcagcaa atgctggaga
    18241 tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac gggcgcggga
    18301 ttagtacact tcctctgatt gatggggtgg aaatcggtac gcttgtggaa ctggcgcaat
    18361 ggacgctgtc agccgataaa gtgctcacat tttaatgttt gagggggcgt ctgacgcccc
    18421 actatgaggg aagtttactc aggcgagtcc cggccgtaac acaccatccg gataattctc
    18481 cgccagcagt ttttttaccg ataccagcaa atcattcagc ccatcgtcct gcattcccag
    18541 tttgataagc tcctgttcca gtgaaaacag gtattgtgcg ttggttccca gtggaccgct
    18601 cgccgccgca atcaacggcg cgatgacctg agcgcgagtg tcagattcat attctggatg
    18661 tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt ccatcatcaa gatcaagctg
    18721 acaccaggtt ggcagataac agccggtaat catctcgcgc ttccacaaca gggttagttc
    18781 ctgctccagc gtctcttctg gcagtcgata ggcgacgcct gtggtgcgtc cgccctcttt
    18841 cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc ccggcggtca ggcgcaggca
    18901 aaacgcacga tgccatccaa ccagtgtacc ggtgcacgac tcggtaaact ccagtgccgg
    18961 attccacatc aacgatccat aaccgaagat ccacaccggt ccctcatcag gtcgacaagc
    19021 cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac cataagagtg attcttcaat
    19081 ggcaccaaat gccgttttac aatcggcatt catcaagaaa tcacgcgtta tcaccttaca
    19141 cctccgccac tgcatacttc cctgcgacaa ctttttggtg tcttccggac atttttactg
    19201 ctcattttga aaccactaac gggacaatat gcaatccacg gaccacttgc aaggcaacac
    19261 cgcacgaata gctttaacta agctaccggt tttacgattt tttatgccat ttatcatcat
    19321 cccctttggc atattcatgc ttcacagcag cccaggccac tttatgcgct gtttcttcgc
    19381 gactggcgtc atcacgccga tcttctttat ctttatattg atcccatgcg ctgttgaacg
    19441 cttctttata gatatcctgg gcatgagacg gaagcacgtg ctttacgctt tccggcagat
    19501 cgcttttcgt tttatacggc atcgttgact ccttttacaa agttgaacat taagtgtggt
    19561 caacaatatc tactcttgcc aaaacataag aattttcatg tcagactctt cttaagaagt
    19621 gctaaaacaa acattgtcta aacgtattac ttatttttat gcccaaatga gtaaaaaatc
    19681 gacagcgggg taaaaattcg taaaatgtta ccgatatcca gacactttct gttatttttc
    19741 ctacctattt tttacatttt cgataaattc atctgcaaag ggataaccat aatgtcaaat
    19801 gctcaagagg cggtaaaaac ccgccacaag gagacttcgc ttattttccc ggttctggcg
    19861 ctggtagtgc tgttcctgtg gggaagcagc cagacactac cagtggtcat tgccatcaat
    19921 cttcttgctc ttattggtat tttaagtagc gcctttagtg ttgtccgtca tgcggacgta
    19981 ttagcccatc gcctgggaga gccttacggt tcgcttattc ttagcctttc agtggttatt
    20041 cttgaagtca gtttgatttc agctttaatg gcaaccggcg acgccgcgcc aacgctaatg
    20101 cgtgatacgc tctattcaat cattatgatt gttaccggtg ggctggttgg cttttcatta
    20161 ttgttgggcg gacgcaagtt tgccacccaa tatatgaatc tgtttggtat caagcagtat
    20221 ttaattgcgc tgttccccct ggcgataatc gtactggtat ttccaatggc tctgcctgcg
    20281 gcgaattttt caaccggtca ggcgttactg gtagcattaa tttctgcggc aatgtatggc
    20341 gtatttttgc tgatccagac caaaacgcat caaagtttgt ttgtctacga gcacgaagat
    20401 gacagtgatg acgacgaccc gcatcacggt aaaccgtctg cccatagcag cctgtggcat
    20461 gctatctggt tgattgtcca tctgattgcc gttattgcgg tgaccaaaat gaacgccagc
    20521 ccgctggaga cattgctcga cagcatgaat gcccctgtcg cctttactgg cttcctggtg
    20581 gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa aagcagtgtt gaacaaccag
    20641 gtccagcgcg cgatgaatct gttctttggt tcagtgttag caaccatttc gcttaccgta
    20701 cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat tgcagtttgc acttggtgcg
    20761 ccagaaatgg tggtgatggt ggcctcttta gtgctgtgtc atatctcctt ctccaccgga
    20821 cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta tttgatgacg
    20881 atatttgcct gacagttata tctcctccga tgacggaaat cgggggagat aatataaatt
    20941 cctgcttatt gtatttcttc atactacctg gacaggttgt taatcagaat tgcgagcctg
    21001 aaccacaacc tgcacgaaac gactgtaaca gctgttgaaa aaccggaatg cactcttcat
    21061 tgcaaacatt tcagggatga agtgaagatg aattgagtcg tagtatccac accaagccgg
    21121 cgttatccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga
    21181 aagagaaaac ccccgcacgt tgagaggtat aacctgacaa cagaacgggg gctaatcttg
    21241 aaccagaaca attcaagaat agccgcaaaa tgttgtcatt acaacaaggc ggctatatga
    21301 cgctcgcgca gtttaccgtg attttctggc acgacctggc agcaccgatc ctggcgggaa
    21361 ttattaccgc agcgattgtc ggctggtggc gtaaccggaa gtaaggcgaa aacgggcgtc
    21421 aggctgccgt gatggcaata tgcgcccgaa accggccgca ggggtaacag cctgcggccc
    21481 tttttgtatc cggcccccat gaaaaaagcc ttaacgcaga acgctaaggc tttagttttc
    21541 aggcaaaaaa gattacttgc tggtatccag ttcttcgaaa cctttcacca agtcatcaat
    21601 cgctttcatc tgcttgagga acggttccag tttagccagc ggcagcgcgg atggaccatc
    21661 acatttcgca tgttccggat ccggatgcgc ttcaataaac agccccgcca gacctaccgc
    21721 cataccggca cgtgccagct cagccacctg agcacgacga ccgccggaag ctgcgccaaa
    21781 cggatcgcgg cattgcagtg cgtgggtcac gtcgaaaatc accggcgaat taccagacac
    21841 tttcttcatg atgctgaagc ccagcatatc gacaaccagg ttgtcatagc cgaagttagc
    21901 accgcgatcg caaagaatca ctttttcgtt gccgccttct ttaaatttat caacgatatt
    21961 acccatctgt cccgggctaa caaactgcgg tttcttgacg ttaattaccg caccggtttt
    22021 cgccatggct tccaccaggt cagtctggcg agcaagaaac gccggcaact gaatcacatc
    22081 cacgacatcc gcaacgggct gtgcctgact tggttcgtga acgtcggtga taattttcac
    22141 accaaaagtc tgcttcaact cctggaagat tttcatccct tcttccaggc ccggtccacg
    22201 ataagagtgg atggaggagc ggttggcttt gtcaaaagag gctttgaaca cgtaagggat
    22261 acccagtttc tgggtcacgg ttacgtagtg ctcgcaaatg cgcatcgcca gatcgcgaga
    22321 ttccagcacg ttcataccgc caaacagtac gaacggcagg tcatttgcta cgttgatgtc
    22381 gccaatgcta accacttttt gtttcatagg atcgccttaa tcttgagtaa aatgtcgatt
    22441 aattaatgca gcacaatatg tttatgcgcg atgttattta tttgcgcacg gatcatttcg
    22501 ctgatcgggt cttccggaca ctgttcaacg aaataactta aatcgttcaa cgcaacgtgt
    22561 tcgcaatcca gttgcgcata aatcaacccg cgatcgcgaa tttcataggg atcttccggg
    22621 ttgaattgta ataaagcttc gctggtgcgt aacgccagct ccatctgatt ctcttccatc
    22681 aacgaagctt tgagtgtatc cagcaatttg cggattacct caatgttatc agcttcatca
    22741 aggtcttcat aaaacagttc cgccgacggg ctgatatttc cctttaacca tacgtccagc
    22801 atatgttcgc ttaacgattc accgttaaaa ggattaatca gccaaatttc gccatccgga
    22861 cattcaatgc gcaatatcag ctgcgtaggg aaaatcaccg gcagcagcgg caaatcaaga
    22921 cgattcgcga cccataataa aacggcacct aatgataccg cactgccctg tcgattcttt
    22981 aacacctggt ccagccataa tgcatcggaa agacgataaa cgccgcgtga ggctttaaat
    23041 ccccagtcgc cgtaaaacag cgcgatcagt ttttccagct gctcttctaa aggcagaagc
    23101 tggctgattt cttccttcgc cagactaacg agacgctcca gttcgtcgta aacatcttgc
    23161 gagggaaaat cgcggcggat tgcttcgcaa gccaggatca tgccttcgca caatggcgct
    23221 ttattaaatt cgaaatcagc taacgatctc atgacttacc ccagtaacgg tacttttgtg
    23281 gtggcgagtt taatgatgat gtacagcacc accaacgcca gcgggaaggc aataatgcgc
    23341 gcctgctgac tgtgcatacg acgataatcg agtgcaataa aacccaaaac gatataaatg
    23401 ataactccaa acagcttttc agtcagccac tgtgcctgcc cggaaaatgg caggatgtgc
    23461 gctttagcca tcaacgcaat gccgcttaac aataacagcg tatcgacaac cggcggcaca
    23521 atgcgcgtcc agcgcgcaaa tgcctgtgga tgcttctgat aacgtagcca gaaacgtaag
    23581 gttaatagcc caactgaaag cgcgatacta ataagatgaa cactaagcag tgtagaaaaa
    23641 cttgtcattg ataatagcgg ccgagcgtta cgcgctcgtt atcaccatag tcccgacagg
    23701 tttcgacgtc atgatatccc gcgtggataa atgcctgtcg caccgcttcg ccctgctgcc
    23761 aaccatgttc cagaagcaga aagccgccgg ataccagcgc gttacgcgac tgttcgatga
    23821 tatgcacgat gtctgccatt ccactgtctg ccgcaaccag cgcagtgagc ggctcaaagc
    23881 ggacatcgcc ttgttgaaga tgtgggtcct gctcgtcaat atacggcgga ttactgacaa
    23941 tcatcgcaaa ctgctgcccg gctagcgcgc taaaccagtc gctttgcaga atatggatgt
    24001 ttttgatcgc cagattctgg gcattacgtt gcgccagggc gacagcatct ggcatacgat
    24061 ctacagcggt aatttcgcag tccggacgct cgctggccag cgccagcgca atcgccccgg
    24121 tacccgtccc gagatcgaga atacggcaag gttgttcagg caaccgcgcc agtgcctgct
    24181 ccaccagaca ctccgtatcc gggcgcggaa ttaaggtcgc tggcgaaaca aataacggca
    24241 acgaccagaa ttctcgcacc ccggttaaat gagcaatggg ttcaccatcg cgacgacgcg
    24301 tcagtagcgc atcaagttgc tgacattgtt cgtcagtcag ctgcgtttca ccaaaggcga
    24361 gaataaaagt acgccctctg ccggtaacat gctccagcag gatttcagca tcacgccgcg
    24421 ggctttcgct cgcctgaagt tggcttattg cttcacgtaa ccagtgttga tattccatta
    24481 ttcctgctcg gacaacgccg ccagttggtc ggcctgatgt tcctggataa tcggttcaat
    24541 cagcatatcc agcttacctt ccatcacttc atccaggcgg tagagcgtca ggttgatgcg
    24601 gtgatcggta acgcgcccct gtgggaagtt gtaagtacgg ttacggtcgc tgcgatcgcc
    24661 actccccagc aggttacgac gggtagacgc ttcggcctgt tgccgttttg ccatttctgc
    24721 agcgtggatg cgagcaccga gtacagaaag tgctttagct ttgtttttat gttgtgaacg
    24781 ttcgtcctga cattcaacaa caatcccggt cggcaagtga gtaatacgaa ttgccgaatc
    24841 ggtggtgtta acgtgctgac cacccgcccc tgacgagcgg aaagtatcaa tgcgcaaatc
    24901 tgctgggttg atgtccggca gttctgcgtc aggcagttct ggcattaccg caacggtaca
    24961 agcggaagta tgaattcgac cctgcgattc cgtagcaggc acacgctgca cgcgatgacc
    25021 gccagattcg aatttcaaac gaccatatac accatcaccg ctaattttgg cgatgatctc
    25081 tttataacca ccatgttcac cctcgctggc gctcatgatt tctacacgcc agcggcgggc
    25141 ttcggcgtaa cggctgtaca tacggaacag atcgccagcg aagagcgccg cttcgtcgcc
    25201 gccggttccg gcacggactt cgaggaaggc gttacgttcg tcatcaggat cttttggtag
    25261 taacagaacc tgtaattgct gttccagttg ctcgcttttt tctttagctt cgcgcagttc
    25321 atcctgcgcc atctcacgca tttcaggatc gtcgagcatc atctgtgcgg tttcgatatc
    25381 ttcctgaacc tgttgccagt cggtaaaaca gcgcgaaaca tcacttaact gcgcatattc
    25441 gcgtgataat gcgcgaaaac gttcctggtc ggcgatagtt tgcgcgtcac ccagcaacgc
    25501 ctgaacttct tcatggcgtt catgcagggc ttccagtttg gcaacgatag aaggcttcat
    25561 aggcgtaaat gcaccctgta aaaaaagaaa atgatgtact gctactccag cccgaggccg
    25621 tcgcgcagaa tattcaggcg ttcgttatcc ccgtcacggg cggcctgttg aagtgatttc
    25681 gttggcgcat ggatcaggcg gttagtcagt ttccatgcca gatcctgcat aatggcttga
    25741 gcgtcaccgc cctgctcaag ggccgctaac gctttggcag ttaactcatc gcgaacgtgc
    25801 tctgcctggc tgcgatactc gcgaatggtt tcgctggcgc tttgtgcacg cagccacgcc
    25861 ataaattcgc tggtttcctg agcgacaata gtttccgcct caaccgctgc ggctttacgc
    25921 tgcgccaggt tgtgcgaaat gatgctttgc agatcgtcca cgctataaag ataagcattc
    25981 gccagtttgc caacttccgg ctcaacatcg cgcggaacgg caatatccac caacagcatt
    26041 ggttggttgc gacggctttt taatgcgcgc tccaccatgc ctttcccgat aatcggtaac
    26101 gggctggcgg tggaactgat gatgatatcg gcttcgcgca gacgttcgtc gatatcactc
    26161 agggcaataa cttccgcgcc gacttcatct gccagaattt gggcacgttc gcgagtgcgg
    26221 ttggcgataa tcatcttctg tactttgtgt tcgcgcagat gacgcgccac cagctcgatg
    26281 gtttcgcccg cacccaccag cagtaccgtc accgtagaga gcgattcaaa gatctgccgc
    26341 gccagcgtac aagccgcaaa agcgacagaa acagcgctgg caccgatatc tgtttcagtg
    26401 cgaacacgtt tcgctacaga gaaagatttc tgaaacatgc gttccagttc gctggccttc
    26461 atatggcctt tttgcgaatc ggcaaacgct tttttaacct gaccgaggat ctgcggctcc
    26521 cccaaaacca gtgaatccag gccgctggca acacgcatta aatggctaac cgcgtcgtta
    26581 tcctgatgcc agtagaggct tttacgcagg tcttcttcat taagattgtg ataatcgcaa
    26641 agccagcgga ttaacgcctc ttgcaggtta tcctgctctt caacgctaag ataaagttcc
    26701 gtgcgattgc acgtcgacag caccacgccg ccctgcacca tcggctgcgc aagcaggctg
    26761 tcaagcgcct gatcgagctt atccggcgaa aacgatacac gttctcgcag cgatacaggt
    26821 gccgttttat ggttgatacc gagtgctaaa agggtcatgt ctgcgggaaa taataccaac
    26881 gttgataggg ttagtctgct tgcatcatac aggatgcgta agatcaataa aagagagcgc
    26941 ccccttttgg agtaattgcc gaaagccgtt agattctggc aattaagaca acttgaacat
    27001 agacgatagc ggacggtaac gctagcatta agggttataa ctgcaacgta tctcaaggac
    27061 ttgtcatcac tatgcccctg cccgattttc gtcttatccg cctactaccg ctggctgctc
    27121 ttgtgctcac tgcctgttcc gttaccacgc ccaaaggtcc tggcaaaagc ccggattcgc
    27181 cacaatggcg tcagcatcag caagacgtgc gcaatcttaa tcagtatcaa actcgcggcg
    27241 cgttcgctta tatttctgac caacaaaaag tgtacgcccg tttcttctgg cagcaaactg
    27301 gccaggatcg ctaccgtctg ctgctgacta acccattggg cagcacggaa ctggagctga
    27361 atgctcaacc gggtaacgtg cagttagtcg acaataaagg ccagcgttat accgccgatg
    27421 acgccgaaga gatgattggc aaattgaccg gaatgcccat tccactcaac agcttacgcc
    27481 agtggatttt aggtttgccg ggtgatgcga ctgactacaa actggacgac cagtaccgcc
    27541 ttagcgaaat cacctacagc cagaatggca aaaactggaa agttgtttac ggtggttatg
    27601 acacaaaaac tcaacctgcg atgccatcca atatggaact caccgatggt ggtcagcgca
    27661 tcaagttaaa aatggataac tggatagtga aataatgcgg acacagtggc cctctccggc
    27721 aaaacttaat ctgtttttat acattaccgg tcagcgtgcg gacggttacc acacgctgca
    27781 aacgctgttt cagttcctgg attacggcga caccatcagc attgagcttc gtgacgatgg
    27841 ggatattcgt ctgttaacac ccgttgaagg cgtggaacat gaagataacc tgatcgttcg
    27901 cgcagcacgg ttgctgatga aaacagcggc agacagcggg cgtcttccta cgggaagcgg
    27961 tgcagaaatc agtattgaca agcgtttgcc gatgggcggc ggtctgggtg gtggttcatc
    28021 caatgccgcg acggtgctgg tggcattaaa ccatctctgg caatgcgggc taagcatgga
    28081 tgagctggcg gaaatggggc tgacgctggg cgcagatgtt cctgtctttg ttcgggggca
    28141 tgccgcgttt gccgaaggcg ttggtgaaat actaacgccg gtggatccgc cagagaagtg
    28201 gtacttggtg gcgcaccctg gtgtaagtat tccgacaccg gtgattttta aagatcctga
    28261 actcccgcgc aatacgccaa aaaggtcaat agaaacgttg ctaaaatgtg aattcagcaa
    28321 tgattgcgag gttatcgcaa gaaaacgttt tcgcgaggtt gatgcggtgc tttcctggct
    28381 gttagaatac gccccgtcgc gcctgactgg gacaggggcc tgtgtctttg ctgaatttga
    28441 tacagagtct gaagcccgcc aggtgctaga gcaagccccg gaatggctca atggctttgt
    28501 ggcgaaaggc gttaatcttt ccccattgca cagagccatg ctttaagccg ggcaagctga
    28561 gtttcggtga caacgtcacc ttgttccaga cgttgcatcg cgctctttaa tacaccgcct
    28621 ggaaagaatc atgcctggcc cgcacagttt tcggcagatt ctttccacca atggacgcat
    28681 gcctgaggtt cttctcgtgc ctgatatgaa gctttttgct ggtaacgcca ccccggaact
    28741 agcacaacgt attgccaacc gcctgtacac ttcactcggc gacgccgctg taggtcgctt
    28801 tagcgacggc gaagtcagcg tacaaattaa cgaaaatgta cgcggtggtg atattttcat
    28861 catccagtcc acttgtgccc ctactaacga caacctgatg gaattagtcg ttatggttga
    28921 tgccctgcgt cgtgcttccg caggtcgtat caccgctgtt atcccctact ttggctatgc
    28981 gcgccaggac cgtcgcgtcc gttccgctcg tgtaccaatc actgcgaaag tggttgcaga
    29041 cttcctctcc agcgtcggtg ttgaccgtgt gctgacagtg gatctgcacg ctgaacagat
    29101 tcagggtttc ttcgacgttc cggttgataa cgtatttggt agcccgatcc tgctggaaga
    29161 catgctgcag ctgaatctgg ataacccaat tgtggtttct ccggacatcg gcggcgttgt
    29221 gcgtgcccgc gctatcgcta agctgctgaa cgataccgat atggcaatca tcgacaaacg
    29281 tcgtccgcgt gcgaacgttt cccaggtgat gcatatcatc ggtgacgttg caggtcgtga
    29341 ctgcgtactg gtcgatgata tgatcgatac tggcggtacg ctgtgtaaag ctgctgaagc
    29401 tctgaaagaa cgtggtgcta aacgtgtatt tgcgtacgcg actcacccga tcttctctgg
    29461 caacgcggcg aacaacctgc gtaactctgt aattgatgaa gtcgttgtct gcgataccat
    29521 tccgctgagc gatgaaatca aatcactgcc gaacgtgcgt actctgaccc tgtcaggtat
    29581 gctggccgaa gcgattcgtc gtatcagcaa cgaagaatcg atctctgcca tgttcgaaca
    29641 ctaatcgaac ccggctcaaa gacccgctgc ggcgggtttt tttgtctgta atatccattt
    29701 gtatgaccta tgcctccttc acctgccatt tagttgacag atgatgcgct cacggatgaa
    29761 acattattgt gaacaaaata ttttcctcac atgtgatgcc tttccgcgct ctgatcgacg
    29821 cttgctggaa agaaaaatat actgccgcac ggtttacccg tgacctgatt gccgggataa
    29881 ccgtcgggat tattgctatc ccgctggcaa tggcgttggc tattggtagt ggtgtggcac
    29941 cccagtacgg tttatatacc gcagctgttg cggggattgt cattgctctg acgggtgggt
    30001 cacgctttag cgtttccggt ccgactgcgg catttgtggt aattctctat cccgtttcgc
    30061 aacagtttgg actggcagga ctgctggtcg cgacactcat gtcaggtgtc tttttgatcc
    30121 tgatggggct ggcacgtttt ggacgcttga ttgaatatat tccggtttcc gttaccttag
    30181 gttttacttc cggtatcggg atcaccatcg gtaccatgca gattaaagat tttctcggtc
    30241 tgcaaatggc ccatgtccct gaacattatc tacaaaaagt cggcgcatta tttatggcgt
    30301 tgccgaccat taatgtgggt gatgctgcca ttggcattgt gacgcttggc attctggttt
    30361 tctggccacg tctgggcatt cgtttacccg gtcaccttcc ggccttgctg gctggttgcg
    30421 cggtgatggg gattgttaac ctgctcggcg gacatgttgc taccatcggt tcgcaattcc
    30481 actacgttct ggccgatggt tctcagggta acggtattcc gcaactgctg ccgcaactgg
    30541 tactgccgtg ggacctgccc aattccgaat ttaccctcac ctgggactcc attcgcactc
    30601 tgcttccggc agcattttcg atggcgatgt taggggcaat tgaatcactg ctctgcgccg
    30661 tggtgctgga tggtatgacc gggacgaaac acaaagcgaa cagtgaactg gttggacagg
    30721 gactggggaa tatcatcgct ccgttctttg gtggtattac cgctacagct gccatcgcgc
    30781 gttctgccgc taacgtccgt gccggggcaa cttcccctat ctcggcggtg atccactcta
    30841 ttctggttat tcttgccctg ctggtactgg caccgctgct ctcctggctg cctctttccg
    30901 ccatggctgc cctgctgttg atggtggcgt ggaacatgag tgaagcgcac aaagtggtcg
    30961 acttgctgcg tcatgcgccg aaagatgaca tcatcgtcat gctgctgtgc atgtcgctga
    31021 ccgtgctgtt tgatatggtt attgccatca gcgtggggat cgtgctggca tcgctactgt
    31081 ttatgcgtcg tatcgcacgt atgactcgcc tggcaccggt agtcgtagac gttccagacg
    31141 atgttctggt tctgcgcgtt attggcccgc tgttctttgc tgccgctgaa ggcttgttca
    31201 cggacctgga gtcacgtctt gaaggcaaac ggattgtgat tctgaagtgg gatgccgttc
    31261 cggtacttga tgctggtggt cttgatgcgt tccagcgttt tgtgaagcgt ctacccgaag
    31321 gatgtgaact gcgcgtgtgc aacgtggaat tccagccact gcgcactatg gctcgcgcag
    31381 gcattcaacc gatcccggga cgcctggcgt tcttcccgaa tcgtcgcgcg gcgatggcgg
    31441 atttataagt acgaaattga ccagtcagcg caaactgact ggtcagcaaa ccgcattatt
    31501 tgttagctat gacggcgatt atcgctacgg cggcaacgtt tgtcatagtg gcgaacccac
    31561 cagtaacggt cgcacacctg ttcatgcccg ccaacacggg cacccgccag ccagagaatg
    31621 gcaccgacga aaatacttag cactgcacca tgtgcgaaat actggggcat attaaactgt
    31681 ggtaactggt tgaggattga ataccccacg ccgaccacca ttaccaccag acccaacccc
    31741 atgagcacgt taccgagtaa cgaagcgttt ttgcgtttca tatgtcacct ccggaacttt
    31801 ctgggttgta acagggaata cccctcttcc ttatgtgtaa agtatagaca acaccctgtg
    31861 ggttatgtgc gggcgtgatc acaattacaa cccttatttc aacaaaactt tacaaataaa
    31921 cgcctgaaca ccttgacttt ctaatagtcc acttaggcaa ttattgcgtt atctgcattt
    31981 gatacgcagt tttttgtcaa gcggggccgc aaacagtaaa ctacgcgcca gttatgtaca
    32041 cactcaggac aaaaaaacgt gacgattaaa ttgattgtcg gcctggcgaa ccccggcgct
    32101 gaatacgccg caacgcgaca taatgctggt gcctggttcg ttgacttact ggcagagcga
    32161 ttgcgcgctc cgctgcgcga agaggctaaa ttctttggtt atacttcgcg agtcaccctt
    32221 ggaggcgaag atgtccgcct gttagtcccg actacattta tgaatctcag cggcaaagcc
    32281 gttgcggcga tggccagttt tttccgcatt aatccggacg agattctggt ggcccacgac
    32341 gaactggatc tgcctcctgg cgtcgccaaa tttaagttgg gcggtggcca tggtggtcac
    32401 aatggactga aagacatcat cagtaaattg ggtaataacc ctaactttca ccgtttacgc
    32461 atcggaattg gtcatccagg cgataaaaat aaagttgtcg gttttgtgtt aggcaaaccg
    32521 cctgttagtg aacagaagtt aattgatgaa gccatcgacg aagcggcacg ttgtactgaa
    32581 atatggttta cagatggctt gaccaaagca acgaaccgat tgcacgcctt taaagcgcaa
    32641 taagtcgttg tctgcggcat ttttgccgag tgccgtgtat aataggcaaa gttatttcca
    32701 tttctgcaat ctgttagcaa taacaggttg attattaaga tattaaggtg atttaaatca
    32761 tgggattcaa atgcggtatc gtcggtttgc ccaacgtcgg gaaatctacc ctgttcaacg
    32821 cgctgaccaa agccggtatt gaagcggcca actttccatt ctgtaccatt gagccgaaca
    32881 cgggcgtcgt accaatgcct gaccctcgcc tggatcaact ggctgaaatc gtaaaaccgc
    32941 agcgtacgct tcccactacc atggaatttg tcgatatcgc cggtctggta aagggcgcgt
    33001 cgaaaggtga aggtctgggt aaccagttcc tgaccaacat tcgtgaaacc gaagcgatcg
    33061 gccacgttgt tcgctgcttt gagaatgaca atatcattca cgtttccggt aaagttaacc
    33121 cggcggacga tattgaagtt atcaataccg aactggcact ggcggatctc gacacctgcg
    33181 agcgcgcgat tcaccgcgta cagaagaaag ccaaaggcgg cgataaagac gcgaaagccg
    33241 aactggcagt actggaaaaa tgccttccac agctggaaaa cgcgggcatg ctgcgcgcgc
    33301 tggatttaag cgcagaagaa aaagcggcta ttcgttacct gagcttcctg acgctgaaac
    33361 cgacgatgta catcgccaac gtcaacgaag acggttttga aaacaacccg tatcttgacc
    33421 aggtgcgtga aatcgcggcg aaagaaggtt ctgttgtggt tccggtttgt gcagctgttg
    33481 aagcagacat tgccgaactg gacgatgaag aacgtgaaga gtttatgcag gaactgggac
    33541 tggaagagcc gggcctgaac cgtgtgatcc gtgccggtta taaactgctg aacctgcaaa
    33601 cttacttcac cgctggggtg aaagaagtgc gcgcatggac catccccgtc ggcgcaactg
    33661 caccgcaagc ggctggcaaa atccataccg atttcgaaaa aggctttatc cgcgcacaga
    33721 ctatctcgtt tgaagatttc atcacttaca aaggtgaaca aggcgcgaaa gaagcaggca
    33781 aaatgcgtgc agaaggtaaa gattacatcg ttaaagatgg cgatgtgatg aacttcctgt
    33841 tcaacgtcta aattccacgt aataccctga cggttaataa tgagccatta tgtgagtcgc
    33901 acataa
//
LOCUS       DAZGTJ010000050        33514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000050 DAZGTJ010000000
VERSION     DAZGTJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.050"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            374..1591
                     /gene="alaA"
                     /locus_tag="ACHK8E_002911"
     CDS             374..1591
                     /gene="alaA"
                     /locus_tag="ACHK8E_002911"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP0345148.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1675..2274
                     /gene="yfbR"
                     /locus_tag="ACHK8E_002912"
     CDS             1675..2274
                     /gene="yfbR"
                     /locus_tag="ACHK8E_002912"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0345149.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2333..4165)
                     /gene="yfbS"
                     /locus_tag="ACHK8E_002913"
     CDS             complement(2333..4165)
                     /gene="yfbS"
                     /locus_tag="ACHK8E_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0345150.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4252..4902)
                     /gene="hxpA"
                     /locus_tag="ACHK8E_002914"
     CDS             complement(4252..4902)
                     /gene="hxpA"
                     /locus_tag="ACHK8E_002914"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP0345151.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4913..5407)
                     /gene="yfbU"
                     /locus_tag="ACHK8E_002915"
     CDS             complement(4913..5407)
                     /gene="yfbU"
                     /locus_tag="ACHK8E_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP0345152.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5491..5946)
                     /gene="yfbV"
                     /locus_tag="ACHK8E_002916"
     CDS             complement(5491..5946)
                     /gene="yfbV"
                     /locus_tag="ACHK8E_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP0345153.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6284..7486
                     /gene="ackA"
                     /locus_tag="ACHK8E_002917"
     CDS             6284..7486
                     /gene="ackA"
                     /locus_tag="ACHK8E_002917"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP0345154.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7561..9705
                     /gene="pta"
                     /locus_tag="ACHK8E_002918"
     CDS             7561..9705
                     /gene="pta"
                     /locus_tag="ACHK8E_002918"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0345155.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9895..11415
                     /gene="yfcC"
                     /locus_tag="ACHK8E_002919"
     CDS             9895..11415
                     /gene="yfcC"
                     /locus_tag="ACHK8E_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP0345156.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11448..11990)
                     /gene="yfcD"
                     /locus_tag="ACHK8E_002920"
     CDS             complement(11448..11990)
                     /gene="yfcD"
                     /locus_tag="ACHK8E_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP0345157.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12048..12599)
                     /gene="yfcE"
                     /locus_tag="ACHK8E_002921"
     CDS             complement(12048..12599)
                     /gene="yfcE"
                     /locus_tag="ACHK8E_002921"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP0345158.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12655..13299)
                     /gene="yfcF"
                     /locus_tag="ACHK8E_002922"
     CDS             complement(12655..13299)
                     /gene="yfcF"
                     /locus_tag="ACHK8E_002922"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP0345159.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13435..14082
                     /gene="yfcG"
                     /locus_tag="ACHK8E_002923"
     CDS             13435..14082
                     /gene="yfcG"
                     /locus_tag="ACHK8E_002923"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP0345160.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14139..14501
                     /gene="folX"
                     /locus_tag="ACHK8E_002924"
     CDS             14139..14501
                     /gene="folX"
                     /locus_tag="ACHK8E_002924"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP0345161.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14522..15415
                     /gene="yfcH"
                     /locus_tag="ACHK8E_002925"
     CDS             14522..15415
                     /gene="yfcH"
                     /locus_tag="ACHK8E_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP0345162.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15463..16365)
                     /gene="rpnB"
                     /locus_tag="ACHK8E_002926"
     CDS             complement(15463..16365)
                     /gene="rpnB"
                     /locus_tag="ACHK8E_002926"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP0345163.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16562..17335)
                     /gene="hisP"
                     /locus_tag="ACHK8E_002927"
     CDS             complement(16562..17335)
                     /gene="hisP"
                     /locus_tag="ACHK8E_002927"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP0345164.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17343..18059)
                     /gene="hisM"
                     /locus_tag="ACHK8E_002928"
     CDS             complement(17343..18059)
                     /gene="hisM"
                     /locus_tag="ACHK8E_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0345165.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18056..18742)
                     /gene="hisQ"
                     /locus_tag="ACHK8E_002929"
     CDS             complement(18056..18742)
                     /gene="hisQ"
                     /locus_tag="ACHK8E_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP0345166.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18832..19614)
                     /gene="hisJ"
                     /locus_tag="ACHK8E_002930"
     CDS             complement(18832..19614)
                     /gene="hisJ"
                     /locus_tag="ACHK8E_002930"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP0345167.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19835..20617)
                     /gene="argT"
                     /locus_tag="ACHK8E_002931"
     CDS             complement(19835..20617)
                     /gene="argT"
                     /locus_tag="ACHK8E_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP0345168.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20883..21452)
                     /gene="ubiX"
                     /locus_tag="ACHK8E_002932"
     CDS             complement(20883..21452)
                     /gene="ubiX"
                     /locus_tag="ACHK8E_002932"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP0345169.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21547..23064)
                     /gene="purF"
                     /locus_tag="ACHK8E_002933"
     CDS             complement(21547..23064)
                     /gene="purF"
                     /locus_tag="ACHK8E_002933"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP0345170.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23101..23589)
                     /gene="cvpA"
                     /locus_tag="ACHK8E_002934"
     CDS             complement(23101..23589)
                     /gene="cvpA"
                     /locus_tag="ACHK8E_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP0345171.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23848..24498)
                     /gene="dedD"
                     /locus_tag="ACHK8E_002935"
     CDS             complement(23848..24498)
                     /gene="dedD"
                     /locus_tag="ACHK8E_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP0345172.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24488..25756)
                     /gene="folC"
                     /locus_tag="ACHK8E_002936"
     CDS             complement(24488..25756)
                     /gene="folC"
                     /locus_tag="ACHK8E_002936"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP0345173.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25826..26740)
                     /gene="accD"
                     /locus_tag="ACHK8E_002937"
     CDS             complement(25826..26740)
                     /gene="accD"
                     /locus_tag="ACHK8E_002937"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP0345174.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26896..27555)
                     /gene="dedA"
                     /locus_tag="ACHK8E_002938"
     CDS             complement(26896..27555)
                     /gene="dedA"
                     /locus_tag="ACHK8E_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0345175.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27638..28450)
                     /gene="truA"
                     /locus_tag="ACHK8E_002939"
     CDS             complement(27638..28450)
                     /gene="truA"
                     /locus_tag="ACHK8E_002939"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP0345176.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28450..29463)
                     /gene="usg"
                     /locus_tag="ACHK8E_002940"
     CDS             complement(28450..29463)
                     /gene="usg"
                     /locus_tag="ACHK8E_002940"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0345177.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29529..30686)
                     /gene="pdxB"
                     /locus_tag="ACHK8E_002941"
     CDS             complement(29529..30686)
                     /gene="pdxB"
                     /locus_tag="ACHK8E_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP0345178.1"
                     /translation="MSRTEKLLTILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(30846..31850)
                     /locus_tag="ACHK8E_002942"
     CDS             complement(30846..31850)
                     /locus_tag="ACHK8E_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0345179.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            complement(31947..32276)
                     /locus_tag="ACHK8E_002943"
     CDS             complement(31947..32276)
                     /locus_tag="ACHK8E_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0345180.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTE
                     TTRKLPR"
     gene            32381..32668
                     /locus_tag="ACHK8E_002944"
     CDS             32381..32668
                     /locus_tag="ACHK8E_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP0345181.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            32864..33202
                     /locus_tag="ACHK8E_002945"
     CDS             32864..33202
                     /locus_tag="ACHK8E_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0345182.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRH"
     gene            33222..33464
                     /locus_tag="ACHK8E_002946"
     CDS             33222..33464
                     /locus_tag="ACHK8E_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0345183.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
BASE COUNT         8406 a         9100 c         8077 g         7931 t
ORIGIN      
        1 aatagtttca caccttgcac ccattacgac acctcggaaa tcatcacgct gttaaactct
       61 ccgtaatgcg gaaatatcat catcacgatt cattagtgaa ttcttaaacg aggcattttc
      121 aaacaattat cttacagata aaaaaaccac agtcacaatt aagaatcaga acaattcact
      181 acataacatt aattactaag catatacacc tcattatttt gccattatta agttattaac
      241 agcacaatcg agtcttcccc tctggcaaaa tcttattctg cagaccttca aaacgccgtc
      301 ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat aacagcaaaa
      361 aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg tctgttacga
      421 catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta acaaggtact
      481 gaaactgaac atcggcaacc ctgccccgtt cggttttgac gcgccagatg aaatcctcgt
      541 tgacgtgatt cgcaacctgc ctaccgctca agggtattgc gattccaaag ggctttactc
      601 cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg ttaccgtgga
      661 agatatttac atcggaaatg gtgtatcgga gcttatcgtt caggcaatgc aggcattgct
      721 gaacagcggc gacgaaatgt tggttcctgc acctgattac ccactatgga ccgcggcggt
      781 ttcgctctcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg actggttccc
      841 ggaccttgat gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg ttattatcaa
      901 cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga tagtggagat
      961 tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa ttctctacga
     1021 cgacgctgag catcactcaa ttgcgccgct ggcacctgat ctgctgacca ttacctttaa
     1081 cggcctgtcg aaaacatacc gcgttgcagg cttccgtcag gggtggatgg tgttgaacgg
     1141 gccgaaaaaa cacgccaaag gctacatcga aggtctggaa atgctggctt caatgcgcct
     1201 gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg ctgggcggtt atcagagcat
     1261 cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg catgggaatt
     1321 gatcaatgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt acatgttccc
     1381 gaaaatcgac gccaaacgct ttaatattca cgacgatcag aaaatggtgc tggatttcct
     1441 gttgcaggaa aaagttctgt tggtgcaagg gacggcattc aactggccgt ggccggatca
     1501 cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga gcaagttcgc
     1561 gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga gattgcatcc
     1621 ggcagtgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt cactatgaaa
     1681 cagagccatt tttttgccca tctctcccgc ctgaaactca ttaaccgctg gccgctaatg
     1741 cgcaatgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat ggtcgcccat
     1801 gcgctggcag ctatcaaaaa tcgtaaattt ggcggtaatg tcaacgccga acgtatcgct
     1861 ttactggcga tgtaccacga tgcctcagaa gtgcttaccg gcgatctccc taccccggtg
     1921 aaatacttca attcgcaaat cgctcaggaa tacaaggcga ttgaaaaaat cgctcagcaa
     1981 aaactggtcg atatggttcc ggaagagttg cgggatatct ttgcgccgtt aattgacgag
     2041 catgcatata gtgatgaaga aaaatcactg gtgaaacagg cagatgcact atgtgcatat
     2101 ctgaaatgtc tggaagaact cgcggcggga aataatgaat tcttgctggc aaaaacgcga
     2161 ctggaagcga cgcttgaagc gcgccgcagc caggagatgg actacttcat ggaagtcttt
     2221 gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct gtaagcagcc
     2281 ggagtctgcg tcgcatcagg caataagcgc cggatgcgac atcaggctct tgtcaaaacg
     2341 gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg aacggtaccc
     2401 ccaacttcac aaagtcactg aagctgtaat ttcccggacc taaaaccagt gtgttaacag
     2461 gtgaagaaac cggcgtcata aaggcggcgg atgctgccat cgccacgacc atcgcgaatg
     2521 ggtaaggcga cacccccatc gttttagcag cagccagcgc aatcggagcc atcaacaccg
     2581 ccgtcgcggt attagaaata aatagcccaa taaccgccga caagacaaac aaacaaccca
     2641 gcatcatatg tggcccgtaa ccgccgccaa tgtccatcag ccctttcacc gccagcgcaa
     2701 cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc aaaataatgc
     2761 tcggccagtg tatggattta taggcgcttt cagcatcgat acagcggaat ttccccatca
     2821 gcaggcaggc gatgatagcg gcaacaggat taggaatttc atcggtcagc attaacgcca
     2881 ccattagcac cagacagaaa atggcatggg gtgcctgact gtgcgcgggc gatgcttcac
     2941 tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgtttggcc agcataccga
     3001 tcagtttcca gttacccaca accaggatga tatcgcccag cagcagaggc tcatccgcca
     3061 gcgaaccttc cagcgccacg ccattgcgct ttagccccac tacattcaat ccgtagcggg
     3121 tacgaaaacc aatttcgcgc accgatttac caataagttc tgactcagga attaatgaaa
     3181 tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc agtaccatcg
     3241 gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca gacatatcaa
     3301 taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg ataacgcgac
     3361 gaaaacgccg ccagcgttca acaccgatga cgttagcacc ataacgttca cgtaatttga
     3421 gatcgtccag tcgttgacca atcattggcg atccggggcg aatagccaga cgtcgcgcac
     3481 gcccggtcag tcgatattca cggataagat cgcgaaaggt tcggcgcgtc cagccttcgc
     3541 gttgtggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata tacaaaatac
     3601 ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag ccgtgatagc
     3661 cttcacgcag caattcactg ttgactacca ggttcggcgg cgtcgccacc agcgtcatca
     3721 tgccgcttat cagcccggca aaacttaacg gcatcatcag acgcgacggc gacgtttgca
     3781 tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgccg gttgaactca
     3841 taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg atttcactgt
     3901 tgcccgccac tttgaccagc catgtcccca ttacagtggc aacaccggta cggaccaaac
     3961 catcgccaat aatgaacaag gcggcaatca ggacaacgtt aggatcggaa aagccggaaa
     4021 atacttcggg gaccgtcagc gttccgctta atgcaaacgc gacaataaca aacaaagcga
     4081 ccgcatccat acgcactctg cccgtcgcaa acaagacgat ggcaacggcc agtaatgaaa
     4141 gaacccaaat caattcaccg ttcacaactt atccttgtta attgaggggg ataacttgat
     4201 tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt ttcactgaat
     4261 aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt ggaggaccaa
     4321 atcgacctca ttcaggcgcg gggtatctgc cggagcgtta accgcaatga catggcaacc
     4381 cgccgccagg ccagaaagca cgccagcggg agcatcttcc accactacac actcctgcgg
     4441 cgcaagcccc agcagctgcg cgcctagcag atacgcatca ggttctggtt ttccgcgctt
     4501 cactcgctca gcggtcacaa acacctctgg cgcgggaagc ccagctattt tatggcgcgc
     4561 tcgcgctacc ggcatggagc cagaagtcac aatggcccac ggaatacctg ctttattcaa
     4621 atgattgagt aaggcgattg cccccggaag tgcggtaata ccctcggttt ccgtggcctc
     4681 gatttgctcc agacgcgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa
     4741 atggcgcaaa gaggtgatcg cctgtttacc gtgaataaaa gccagcacct cttccggcgc
     4801 taacccatga cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac
     4861 cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcgt
     4921 tgataatttg attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaca
     4981 cattaagcat gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccgtgagttc
     5041 cggcatcaaa atgggtatag cgtccttcca cattaaccat aaagcggaca taaccgaggt
     5101 aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt
     5161 gctggtcctg caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga
     5221 tgatagtgcg gcaggtttct tctttcagct cgccaaactc gcgatccagt tcacgcatct
     5281 gtaatccata accacgctca atgattgttt gcaggcgacg gtaacgttca gcatttgccg
     5341 gatcgagcat agtcatcatc ttgtactggt tagacaaaat aagacgttgc gcgttggtca
     5401 tttccattgt tgactcctgt atcactctac tacggtgaaa aaaaaagaag gctgagtatg
     5461 ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag
     5521 ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac
     5581 gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa accagttaag
     5641 gattgcaggg ggtaatggcg ttacagaacg tttgcccagc caccacaatc cctgcatggg
     5701 taaacttaag gcgaacagcg cagtggcaac tgccggccca agctgaccgc ccagggcaat
     5761 ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat
     5821 cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt
     5881 tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt
     5941 tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact
     6001 cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca
     6061 gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat
     6121 tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa
     6181 atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc
     6241 tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt
     6301 actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg
     6361 tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg
     6421 gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc
     6481 gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac
     6541 tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga
     6601 tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc
     6661 tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt
     6721 tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct
     6781 gccgtacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca
     6841 cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat
     6901 catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt
     6961 tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga
     7021 tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat
     7081 caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg
     7141 ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta
     7201 ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga
     7261 cgctgttgta ttcaccggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct
     7321 gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt
     7381 cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa
     7441 cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag
     7501 ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc
     7561 gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc
     7621 cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc
     7681 gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac
     7741 tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc
     7801 agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa
     7861 gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag
     7921 tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag
     7981 ggcactgaca ccccggaaca gctgaaagag cgtattgaac tgacccgcaa cagcttcggc
     8041 ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaatgc accggttgat
     8101 gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa
     8161 gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgcg
     8221 gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat
     8281 gcaaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc
     8341 gcacgcagca ttccacacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc
     8401 gcagaccgtc ctgacgtgct ggttgccgcg tgcctggctg ccatgaacgg cgtagaaatc
     8461 ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa
     8521 cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacttct
     8581 ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgagcg tatcgagaaa
     8641 gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgattctct gactgccact
     8701 tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt atcagctgac tgaacttgcg
     8761 cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac cgttaaggca
     8821 gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag
     8881 atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg caggcattga aatcgttgat
     8941 ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa gaacaaaggc
     9001 atgaccgaaa ccgttgcccg cgaacagctg gaagataacg tggttctcgg tacgctgatg
     9061 ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc
     9121 atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg
     9181 ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat
     9241 ccgaccgcag aacagctggc agaaatcgcg attcagtccg cagattccgc tgcggccttc
     9301 ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg ggacttctgg tgctggtagc
     9361 gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg
     9421 atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagca
     9481 ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt
     9541 aacaccacct acaaagcggt acagcgttct gctgacctga tctctatcgg accgatgctg
     9601 cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc
     9661 tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatccg
     9721 cagctttacg ctgcggatat ctgaaccgga aatagtcact atttccggtt ttttattctc
     9781 ttaatttgca ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg
     9841 ccatctcaat ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc
     9901 gcaatcactg aatctaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc
     9961 ttttttgttg ccattttaac cagccttgcc acctgggtag ttccggtggg catgtttgac
    10021 agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca
    10081 cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg
    10141 ttcacgacgg gcgatgaacg cccgggtctg atgaacttcc cgtttgaagg gttaacctca
    10201 ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg
    10261 tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat
    10321 acccgcggaa atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc
    10381 gcaatatttg gtatgggaga agaggccgtc gcctttgcca ttattatcgc accgctaatg
    10441 gtccggctgg gctatgacag tattaccacc gtcctggtga cctatatcgc cacgcaaatc
    10501 ggttttgcca gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg gattgccggc
    10561 gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc caccctgatt
    10621 ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc
    10681 gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt
    10741 acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg ggtgatttgg
    10801 ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt
    10861 ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg
    10921 gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc
    10981 gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat
    11041 accatcctca acagcattgc caatgccatt agcggtctgg ataacgcggt cgcggcctgg
    11101 tttatgttgc tcttccaggc ggtatttaat ttcttcgtga catctggttc tggtcaggcg
    11161 gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgtaaa ccgtcaggtt
    11221 accgttctgg cgttccagtt tggtgatggc ttcagccaca tcatttaccc gacctcagct
    11281 tcgttaatgg caacgctcgg tgtttgccgg gtggacttcc gtaactggct gaaggtgggc
    11341 gcgacactgc ttggactgct gtttattatg tccagcgtcg tggtgatcgg cgctcagttg
    11401 atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt
    11461 tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc
    11521 gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact
    11581 tcgctgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct gaacaacgcg
    11641 ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca
    11701 ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg
    11761 gcctggacta caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg
    11821 gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga
    11881 tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct
    11941 tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa
    12001 ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc
    12061 gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc
    12121 agcatgccat aactcgccgg attaccgcct ttaggaatac tcaccgagcc ggggttgaag
    12181 tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta caccagcaca
    12241 tcgttctggt ttaaagcagg taggttttcc gggccaaaca gatgaccatg cgtcaaaaac
    12301 agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcaaaagc
    12361 atttgatcca cttcgctgtc gcaattgccg cgcacagcga taaccttatg tgccacttca
    12421 ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg
    12481 tgattcaaca cgtcgccaag gatcaccagc cactgggcac cactttgggc aaacaactcc
    12541 agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc
    12601 actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc
    12661 agattgcttc gccgaaagag caataaaacg ctggacagac gctcgctgcc actggaatgt
    12721 tgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc ggttaatcat
    12781 tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc
    12841 taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct ccgccgttaa
    12901 tggcgctttt ttcgcacccg caaaaacgac atctgtcgaa cgttcttcgc ggatgggcat
    12961 cagatcgcta cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag
    13021 cggataaatg cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc
    13081 agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc
    13141 gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg
    13201 aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag gggagaaaaa
    13261 atgggcatct gaccaaagcg tgatagcggg tttactcatt atgacctcgg caataaaggg
    13321 ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgcaag
    13381 tcgccgcgct ttctatactt gtggtgttca gatgattaga caaccggagc tgcaatgatc
    13441 gatctctatt tcgccccgac accaaatggt cacaaaatta cgctgtttct cgaagaagca
    13501 gggctggatt atcgcttgat aaaggtagac ctggggaaag gcggtcagtt tcgcccggaa
    13561 tttttgcaca tttcgcctaa caacaaaatt ccggcaattg ttgatcattc tccagccgat
    13621 ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct ggctgagaaa
    13681 acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc
    13741 tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc
    13801 tcccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctgtaccat
    13861 gtactgaaca agcggctgga aaactcgccc tggctgggtg gcgagaacta cagcattgcg
    13921 gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga cctcgcaatg
    13981 tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac cggtcaggca
    14041 ctgttaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg
    14101 tattatttca tcctaagaaa aacaacggag aacctgcaat ggcacaacct gccgctatta
    14161 ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa gaaattaaca
    14221 accgtcagga tattgttatc aatgtgacga ttcactaccc cgccgataaa gcgcgcacca
    14281 gcgaagatat caacgatgcg ctgaattatc gcaccgtgac gaaaaacatt attcagcatg
    14341 tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac
    14401 gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct
    14461 acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat cgggaggctg
    14521 catgaagata gtgatcaccg gagggacagg attaattggt cgccatttga ttccacgttt
    14581 gctggagctg ggccatcaaa tcacggtaat gacgcgtaac ccgcagaaag ccagttctgt
    14641 tctcggccct cgggtggcac tatggcaagg gcttgccgat caaagcaacc tcaacggcgt
    14701 tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga ctcacgagca
    14761 aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa
    14821 tgccagcgac acgccgccgt cggtactcat ttccggctcg gcaacgggct attatggcga
    14881 cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta ctcataaact
    14941 ctgcgcccac tgggaagaaa ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct
    15001 gctgcgtacc ggcgtagtgc tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc
    15061 gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg cctggattca
    15121 tattgatgat atggtcaacg gcattctctg gctgctggat aacgagctgc gcgggccatt
    15181 taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc tcggtcatgc
    15241 tctgcatcgc ccggctattt tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga
    15301 atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg
    15361 ttttgcgttt cgttggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg
    15421 tttactgcaa acatccgcca gttaactccc ggtgttacag ggttagtggc tttgcgcgat
    15481 aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctcggtcta aacctctctc
    15541 cagcatctcc tgagcaatac gcagggcttc ttcgcgtttg ccaagaatta atccatcatt
    15601 ccgcccttct tcacgtaatc tgtcagcaat agtcatcagt ttctcctttt cttgtggtgc
    15661 gcgttccgct atctcaccaa taaatgcacg gaaacgctgg gcatcccccg tttgtaatac
    15721 gtaattaaac agggctttta gctgtctgtc attagtgttc cctgtaacta gcagcgaaac
    15781 aatttggtca actaatccca acagatcgcg ctgacgaata tgtttctgaa ttaactccaa
    15841 cagcgccatt ttgcgatgtt gcataatctc gtcatccggc accacggtaa tatctaccag
    15901 cggaaaagcc gatgaataaa ttttgcgggc tatagcaggc tcggcaaatt catcaagcca
    15961 gcaaagtgaa taaggataag gacttctgca accatgataa aacagcatcg ggatcaccaa
    16021 tggaagctct ttatagcctg catcaagatg gttttgcatc gccgcaatgg aatagcgcat
    16081 catgcgaaaa gccattaatt cttccggctt actttggtgc tctatcacta cataaatata
    16141 acctgctccc tcctgcgttt tcacagacca caagaggtcg gaataatatt gccgcaggtc
    16201 ttcatcaata aaactgtttg gttccagttt tagcgttgtt aaatcacaca gtttgcgcag
    16261 cggcgcggga agatgaatat caataaaatc ccgcgcggtg tctggatggc gtaaaaaaga
    16321 tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc
    16381 ctcaaattta agatgacgcg acaataacca cgattaatac ccaatgcagc aggcagtttt
    16441 tatctttgga cagcgaattc agaactattt ttagcggact gcaactttta catttcagcg
    16501 gaagctgact cgcaaattcc ccttgaaaat actccggcag cgttagccac cggaggggga
    16561 attatttcag cgatccctta aggaactgtt gcagacgagg gctttgcggg ttaccaaata
    16621 actgttctgg cgcgccctct tcttctattt tcccctgatg gaggaaaatg acgtgagtag
    16681 aaacatggcg agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg
    16741 ccagttgctg cataatacgc aacacttcgc ctaccagttc aggatcgagc gccgaggtag
    16801 gttcatcaaa cagtaaaact tccggttcca tcgccagcgc ccgcgcgata gaaacacgct
    16861 gttgctgacc accggacagg tgcaccgggt atttcccctg cgcacgttcg tctatcccga
    16921 cttttgccag atatttcacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct
    16981 gaatcggcgc ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct
    17041 ggaataccat cgtcaggcgt gtgcgcagta agcgcagctg atttttatcg gcgactttga
    17101 gttgaccatc tttgtcgcgc accagattga tcgtctggcc attgaccacg atcgatcctt
    17161 cgctcggttt ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc gatcccgacg
    17221 atccgatgat gctaattaca tctccggcat tcgcttgcag tgaaacccct ttcagcactt
    17281 cgtgttcgcc gtagcgttta tgcaaatcga taacgtttaa tttattctcg gacatcgtat
    17341 tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag
    17401 aggctgatca ggacataaga gatgattaaa tagagcaccg cggcaatgcc gaaggcggta
    17461 aaaggttgat atgtggcggc gttaatatcg cgggcaattt tcagcagatc cggcaccgtg
    17521 gcagtaaatg ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtacgccggt
    17581 aacgcaatac gcagcgcaga aggcaaaata atgcagcgat acattttaaa ggtcgagaag
    17641 ccataggctc tggcggcttc aatttcccca tgcggcactg aacggattgc cccggcaaaa
    17701 atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcagacca
    17761 ctgcgaaaaa aggcgttaag gaactcggtt cccttaacaa tctccagcgt gtacatgccg
    17821 gaatagaaca ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac
    17881 cagattggaa actggatgta tttattactt gagacgcgac caatcgccag aaacagcgcc
    17941 agaactccgc ctatcactac cgacaaaata agcagccaca gggtgatcgc cacgccagta
    18001 aagcgataac cgtcggtcca cagcagtggt ttccagtatt catgtaagat ttcgatcaca
    18061 ggtcagccct cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg
    18121 aaacagtggt gaaaaccagg taaatcacgc cacagacgat ggcgaaatag aacggttccc
    18181 aggtactttt gcctgccagc tgagtggctt tgaccacatc ttccaggccg agtaacgaaa
    18241 cgagtgcggt agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac
    18301 gcatcatcgc cggaaacatg atccgtcgaa acacttgccc acgagtaaaa ccgaacgccg
    18361 tcgccgcctc tatgtgtcct ttcggcactg ccataaacgc gccacgaaac gtttcggtaa
    18421 aataagcacc gtagataaaa ccgagagtga taatacctgc gaccatcgga tcaatatcaa
    18481 tctgcccgac gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa
    18541 tcagcagcat caacaccaag tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa
    18601 tcagccccga aagccgattt tgcgagagct taccgccagc gccaattaaa ccgataatta
    18661 cagcgagcac tacagagctg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa
    18721 cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct
    18781 tgtctccctc cggttacccg aagggagcgg actttctttc accgatggcg attaaccacc
    18841 ataaacatca aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat
    18901 ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta tcttctttac gcaggcccat
    18961 gccggtccct acgccaaaca gtttttcatc tttaacagac ggaccaccga atttgtaatc
    19021 tttaccgacg ggttgtttga ggaaaccttc gctggcagcg acctcatcct ggaacgcggc
    19081 atcaatacgt ccggcagtca ggtcagaata aatgttgtcc tgcccctgat aagaaacgat
    19141 ttcaatgcct tttggtgccc aatgttcgtt accgaacgtc tcctgggtgg tgccctgcaa
    19201 tacgcctacc cgtttgcctt tcagcgactc gactgtcggc tgaatgtcgg aatttttcgc
    19261 caccaccaga cgagaatcgg cagcgtacag tttgtcggtg aaggcaattt cttgctgacg
    19321 tttttccgta atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg
    19381 gattaacgca tccagtgggt tttcgacaaa cgtacattgc gtattgatgc gtttgcacaa
    19441 ctccttcgcc agatcgatat cgaagccaac cagttcgcct tgtgaatttt ttgattcaaa
    19501 tggcgcatag gtcggatcgg taccgatgcg gatgttttgc ggaatcgcag caaacgccgc
    19561 agttgcgctg gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc
    19621 ctcaactgac agtcttttta tagggaatat ttacaggttt gttacactta tcgtgccata
    19681 aaattgacca tcaaggcaaa atatcccgcc ccgttagacg tattattttt cattaaaaat
    19741 gcttaagtat gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg
    19801 atcattccat caggtacagt ttccctgcga cgtatcagtc accgtagaca ttaaagtcga
    19861 aatacttttt cgccatcttg tcgtaggtac cgtcctgacg cagctcgcca agcgccttat
    19921 taaacgccgc cgtcagttca gcatcatctt tacgtagccc tacaccggta ccgtcaccga
    19981 agtatttttt gtctttcact gatgagccag caaaggcgaa atctttacca gcaggttgct
    20041 tgaggaaacc ttcgctggcc gccacttcat cctgcagtgc ggcatccaga cgtcctgcag
    20101 ccagatcgga atagaccaaa tcctggttgg cataggccac cacatccaca cctttgctac
    20161 gccaggtctc gttagcgtaa gcttcctggg ttgatccctg cagcacaccc acatgtttac
    20221 ctttcagtga atccagcgtt ggctgaatcg gcgaaccttt ggcggcaatc aaacgagaat
    20281 ctgcggcgta caacttgtcg gagaaagcaa tctcctgctg acgtttatcg gtaatggaga
    20341 gcgacgaaat aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt
    20401 cactggcaac ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga
    20461 tatcaaagcc aacaaagtca cctttggcat ctttcgatga gaacggcgca taggtggtat
    20521 cggttccgat acgtaccgtc tccggtagcg ccgcatagct ggaagccact gcggagagac
    20581 cgactaacaa agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg
    20641 cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc
    20701 atctttacga caacagtaac attcaacgtt aaatatgtta ataagacgtt gcattattgt
    20761 cctaaagttg aagatagcag gtatggcggt tgaatcgcag cgtttcgatt catcgaaagg
    20821 aacaaaatgt cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacaatga
    20881 ggttatgcgc cctgccagcg ggcaaagaga tcttcaggaa gggttatcgc aaactggtca
    20941 agaacacgat taaccgtctg atttatcaca tcatcaaggg attgtgggcg atgataaaac
    21001 gccggaacgg gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga
    21061 tggcctaagt gcagtggcgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc
    21121 accacatctg ccgcacgggt cagtaagcca tcagtatagc tatggacaat gccggacagg
    21181 gttttgattg aacagggtaa aatcaccatc cccaacgtct ggaaagaacc ggaagaaatg
    21241 ctggcagcaa tatcgcgcgc atcgtgtgtc acatcagcta acgcctgcac ttcgcgcagg
    21301 gaaaaatccg tttcgaggga taaggtctgg cgcgctgcct ggctcattac cagatgcgtt
    21361 tcgatatctg tgacatcgcg cagaacctgt aataaacgca cgccataaat cgcgccgctg
    21421 gcaccgctga tgcctacaat gagtcgtttc ataaatagtt gccctgtcag acttgcaggc
    21481 agactttgca ggatttcgac gggagttgca agtcagggtg ccagaccggc accctgagcg
    21541 aaggcatcat ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc
    21601 tttggcgtcg tcattacgta aagtatcgag gaaatcgagg tagccctgat caacgtcttt
    21661 ggtgacgtag acgccgttga ataccgagca ttcaaactgc tggatatccg ggttttcagc
    21721 gcgaacggct tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat
    21781 ctggcgaatt tcatctactt cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc
    21841 gataccgtaa acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt
    21901 cgctccggct tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacaatgga
    21961 gtcgtcgacc agcaagacgt ttttatcgcg gaactcggcg cggttggcgt tcagtttacg
    22021 gcgcacggac ttacgacgca gctgctggcc cggcatgatg aaggtgcggc caacatagcg
    22081 gtttttaacg aagccctggc ggtacggttt gcccagaata cgagcaattt ccagcgcgat
    22141 atcacacgag gtttccggaa tagggatcac cacgtcgata tccagatctt cccattcgcg
    22201 ggcaattttc tcgcccagtt tggtgcccat attcacgcgc gcgctgtaaa cggaaatttt
    22261 gtcgatgaac gagtccgggc gggcaaagta tacatactca aacaggcacg gattgctgac
    22321 cggattgtca gcacattgac gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc
    22381 gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcaa cgctttcgga
    22441 agcgaccata tattctgtac ggttctcgtc aatatcacgt tttcccagta ccaacggacg
    22501 aataccgttt ggatcgcgga aagcaaccat accgtggccg atgatcatcg ccacacaggc
    22561 atacgcgccg cggattaagc ggtttgtagc agcgatggcg gcgaaaatgt tgtcggcttc
    22621 cagcggatag tggcggaagt tgtccagttc gctggcaaag atattaagca gaatttccga
    22681 gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg
    22741 agcgttggtc agattgccgt tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa
    22801 cggctgcgct tcagaggcgc tggagctgcc agccgtaggg taacgcacat gaccaatgcc
    22861 catattgccc tgcaaacgct gcatatggcg agcttcaaat acatcgctca ccagcccgtt
    22921 cgctttacgc aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg
    22981 accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc
    23041 ggcgataccg acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac
    23101 ttacgctctg ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat
    23161 aaaactgaac tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt
    23221 aaaggagtcg agaaagaaga gaatggcggc aacaatcaac acaccgcgca acgcaccgaa
    23281 acagacgccc agcacccgat cggtgcctga caaccccgtt ttctctacca actggcctat
    23341 cacgaagttc acgatagcac caacgatcag ggtagcgata aacagtaccg cgatggcaat
    23401 cccatttcga accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta
    23461 atgactggca acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa
    23521 gccgcggatc aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat
    23581 ccagaccata tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac
    23641 agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc atcaataaaa atggcgctga
    23701 aaaaacattc aacgccatcg actttttatg cctttgcggc atcgggcaat gcgtgtcgga
    23761 tgcggcgtga acaccttatc cgacctacgg ttctacccct gtgtaggcct gataagacgc
    23821 gccagcgtcg catcaggcaa gaccgtatta attcggcgta tagcccatta ccacgccact
    23881 taagccagaa agttgcttca actcacccag cgatcctttc agcttatctt tcgaggcatc
    23941 aggcccaacc agaatacggg taattttacc ctgcactggc gtggatggcg acgtataaac
    24001 ccgataaccg gcaccgcgca gcttaccgac aatctcattc actttatcgg catttttcag
    24061 cgcgcccagt tgcacaacat aggctttacc cgttggcgct gctttttctt ccacgaccgg
    24121 cttcgacgca ggtggtgctt caaccttcgg tttaggcgac tccaccggtt tcggcttcgg
    24181 tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttgactgcaa tagtggccgg
    24241 atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc cttccggcgg
    24301 ctgcgtcggc agcgcctggg tggcggctgg catcatatca ggctcatcgc gatcgcccgc
    24361 tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct gcccgtccag
    24421 cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca ctaaccgatt
    24481 ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca tgtgcgaccg
    24541 tgtggaacga accacacacc agcacggtat cttccgcttt agcgtccgcc attgcggcat
    24601 cccatgcctg cgccacgcta tcaaatgatt tgccgttacc caaatgctca agcagttgtt
    24661 ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca accacgcttt
    24721 tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata accgccagca
    24781 cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc gcatgtggat
    24841 tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa cgtcccggca
    24901 aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacctccagc ccgctggcac
    24961 gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac aagcggcaac ggcagatttt
    25021 ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa tagttccact
    25081 caacgccccg acgttgtaac agtgcacttt tttcctgcgc cacatcagca atggtagaag
    25141 gcatttccgg ctcaccgaca attgccggtt tttcgctgcg gaagatgcct gctttctcgc
    25201 gaccaatgct ttcgcgatct ggccccagcc agtcggtatg atccagcgca atgctggtta
    25261 ctaccgcaac atcggcatcg acaatattgg ttgcgtccag acgaccgccc agccctactt
    25321 ccagaatcac cacgtcaagt tgtgcctgtt tgaacagcca caacgccgac agcgtaccgt
    25381 actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca aaagaggcgg
    25441 tgtgggccga ttccggcaat tcctggccct gcacacgtac gcgctcggta taacgcacca
    25501 gatgcggcga agtgtagacg cccactttgt accctgccgc catcagaatc gactccagcg
    25561 tacggcaagt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat ggcgcaggtt
    25621 tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga tcgatagttt
    25681 tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg gcttgaggag
    25741 tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa aagggcagag
    25801 ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatctggt accggtggca
    25861 ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc aacttcgcca
    25921 gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg atcgcgcctt
    25981 tctcgatcag gaattcactg cgctggaatc ctggcggcag tttttcgcga acggtctgtt
    26041 cgataacacg cggaccggca aagccgatta acgctttcgg ttcagcgatg ttgagatcgc
    26101 ccagcatggc gaaacttgca gaaacaccgc ccatggtcgg gtcggtcagc acggagatgt
    26161 acggcaagcc gcgctcctgc atttttgcca gcgccgcaga ggttttcgcc atctgcatca
    26221 gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag atcagtgggc
    26281 agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacctacc acagacccca
    26341 tggaaccgcc cataaaggcg aactcgaatg ccgcagcgac aactggcatt ccatacaggg
    26401 tgcctttcat caccaccagc gcatcttttt cgccggtttc tttctgcgca gatgccagac
    26461 ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca agctcgctac
    26521 ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc gctgtcatac
    26581 gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca gcgcggtata
    26641 aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc gccttgcggg
    26701 tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt tctgtctgaa
    26761 cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc ccccaacaga
    26821 ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa agtggtcgaa
    26881 ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtggc gaataaattc
    26941 gaaaacacct ggcaatattg aaacaaaaat aatgccaaca atcatcagtt taaggttgct
    27001 ctgaatgaag gggagcgttc cgaagaaata acccgcgtag gtaaaaatca gtacccatag
    27061 caacgcgccg atgacgttaa aagcggcgaa atgacgatac gacatgtggc ccattcccgc
    27121 aacgaacggc gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa tcgttttgcc
    27181 gccatgtttc tcataaaact gatgagtctt atcaagataa ctgcgacgaa agattttcga
    27241 atccggatta ctgaacagtt tgtcaccaaa taaccgtcca atggtgtagt tcaccgcgtc
    27301 acccacaatc gcggcgatca gcatcagcac caccatcata tggacgttga gatcgttggt
    27361 ttccagtgat gccaacgcac cggcaacaaa cagcaatgaa tcacccggta aaaacggcgt
    27421 taccaccaga ccggtttcac agaacaaaat taagaacaaa atggcataaa cccagacgcc
    27481 atactccgcg accagttctg ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa
    27541 ataaatcagg tccatattag cctatgcctt gtactcgtca tctttcaggc tgtaactacg
    27601 ttggctacag cctgaaatgt tccgagcatt attcttgtta gtccgccaga aatagcgggc
    27661 ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc agatacagcc
    27721 cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc aactctgcta
    27781 tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca atattcctga
    27841 ccatatgatg tacaaaggca ttcgctttga tatctaccac cacataagga ccgtgacgcg
    27901 tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc accgcacgga
    27961 acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt
    28021 ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc agccgatgat
    28081 tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga aaatcatcag
    28141 gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt acgcccaacg
    28201 tccacgcagc gtctttgcgc tgtgcggtcg tttcgaaatg cacaacctgc ccggtaccgt
    28261 gtacaccggc gtcagtacgc ccggcgcaga agacggtgat gggttcgttc gccacctggg
    28321 agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt tgccagccgt
    28381 aatacctact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc ggttgttgct
    28441 ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta ctgccatcag
    28501 cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tgaacttgct ccggcatacc
    28561 gtagtcatta cgtacgcagc caacagaaag atgcggcgta ccagaagcat ctcctacctg
    28621 agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa acgcatcacg
    28681 cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg catgaccgta
    28741 gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct gcaggatttt
    28801 gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat ccggcagtaa
    28861 cggcagcatg ttgaacgcca gctgacgccc gaagaaatct tcttcgtcaa tcggaatgcc
    28921 attgagtaat ttagcactct gccccgctaa cgcatcgacc gcttttttgc cctgggcgga
    28981 ggctgaaatc aggctggtaa cgctgatacg tgataaaccg ccctgatcaa ttagcggttt
    29041 cagtgccgcc agcagctggc tggtcagact gtccggtacg gcgatgacat tccggttgcg
    29101 gtaatcggtc agtacaaacg ggtttacttc cggcactacc agcggtacgt caggttcgag
    29161 agcaaacaat ccgctgctgt cgatcaccag acaacctgag ttggtcgctt cttcaaccca
    29221 ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcaaa
    29281 ttcagcggca tcctgcacgg tgattgtctt accaccaaag cgcagttgtt cgcctgcgct
    29341 ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag ccagcgtttc
    29401 aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc agccttcaga
    29461 catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca gagagcatga
    29521 agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca cagcaatgat
    29581 gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg ctcaagatag
    29641 tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag tggcgcatca
    29701 tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg atcgagcgga
    29761 ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag cgcaacgtgt
    29821 tgttcatgcc caatgaattt gctataggct tcaaacactt gcgtagtacc gcgtgcttta
    29881 ccttccaggg tatagcctgc aatatgcggc gtgccgatat ccacttttgt cagcaattcc
    29941 acattaagtt ccggctcgcc ttcccagaca tccagcacca cgcttaactt ctggccttca
    30001 ttcaggcagg tcagcagcgc ggtattatcg acgactgcgc cacggcaggc gttaatcaga
    30061 atcgctccgg gcttcaggct acggatcagt ttttcatccg ccagatgtcg cgttttgtac
    30121 ggaccatctt taaagagtgg cgtatggaaa gtcagaatat ccgcatgctg gactaattca
    30181 tccagcgagc ggaaatcgcc ctcatcccca cggtcggcgc gaggcggatc gcaaagcaag
    30241 gttgtaatcc ctaacgcttc cagtcgtgcc tgtaaacgcc gaccaacgtt accaacgccc
    30301 acgatcccca cggtacgctc gtgcagtgaa aatccatcgc gttcagcaag catcagcagt
    30361 gaggagaaaa catattccac tactgcaatc gcattacagc caggtgcagc ggaaaaacca
    30421 attcccgcct gctttaacca tgtgtcatca acatggtctg tccccgcggt ggcagtgcca
    30481 acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac cgaacgcacc
    30541 atcagcgcat ccgcgtctgc cagttgagcc acggggatgg gacgcccggg aaccgcggtc
    30601 acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc aacaaggatc
    30661 gttaataatt tttcagtacg cgacaatctg gccccccctt tatcagcaaa caccctttta
    30721 accccaccgt acaacatgaa aaccatttca taatcataca catacaaacg caaccattta
    30781 gacgggtgat actggaaagt atgtttcaca gtatcaccct taaagctatt tcagaccaga
    30841 caaaatcact ctcccgtgga caccgagtgg acacttacgc cacctttcag cggattaaga
    30901 gccaccgcat cctgcaaata atccggcgca aaatgtgcat acgccattgt ttgctggata
    30961 gtcgcgtgac ccaggatcct ctgtagcgca ataatgtttc ccccgttcat cacaaaatga
    31021 ctggcgaacg tatgtcgcaa cacatgcgcc gcctgtcctt ttggcaaatc gggtttaacc
    31081 cttttcagca ccaggcaaaa ttcccggtac ttcacatcaa ataatccccc cgtctcgctg
    31141 gttttgatcg cctcacaaac cgcctgcgag attggcaccg ttctcttacg accgtttttg
    31201 gtctcaagaa acgtcacacg attatgaact atctgttcac cacgaagctt actggcttcg
    31261 ccccagcgtg cccccgtact taaacacaaa agcgcaacac gccagtaatc gccctctagt
    31321 gtatcgagca ataacgccac ttctttctgg gacaagaaag ccatttctcg cggggacaca
    31381 taaagcacag agattcccct gaccgggtgc tctgcatccc atagtcctat ttttttcaga
    31441 gcggtaaaca ttccggataa ccggttcata tacctgttag ccgatgacgg cttcagtccg
    31501 tcagcgatcc tgcgagaacg ccaggcaatg attttcagct tatccagatc caccgcctgc
    31561 atatcagcac caagttcatt gattatgttg cgtaattgtt ttcggtcttc ctccgcttta
    31621 cgcctgtgct ggccgtgata cacccaccac aactccagca aatcgttcaa cgtccggcga
    31681 tcacggtagc cctgtatata ttcccgcttc tcagcattcg ccatgatgta gcgctcagtg
    31741 gccaccgcta ccgatttttt gttaaatacc ttacgcacgc gttttccctt gcgtccgttc
    31801 ggcctgatat ccagcaaata acgaccatct tcgagcttct taatcgacat ggcgaagccc
    31861 tccaatgaac cgttttacaa tttcaccagc ttcttcccag caatagtcag accaggcaaa
    31921 aagcaggtcc aaccagtttt ctggcctcag cggggtaatt ttcgggttgt ttcggttgag
    31981 tcctgattgc cctccaaatt gcaggagcca tcaagagaga gagccggggc tatctgtccg
    32041 gcctccggca ttgtttcatc agttgataac cagtaggcgt acttcttaaa tttagggtgt
    32101 tttgtaacct taagtaaggc cccctctgtt acttgcttcc ccctcacctc atagttagtt
    32161 actgtcccat aaggcaaccc cacgcaatcc gcaaactcct ggcgggtcat accttcagct
    32221 tcacgtatca ggcgaaattt ttcacccatg cttgacaaag atgccatatc gggcatatcc
    32281 tccattgcaa acgtgccaca tcgggcacac acataagaag ccaaaataag ccactttggg
    32341 ccattttgag ccattcgaac gaatcaagga gattaccaca atgagagaat cagagcttga
    32401 gggcttcatt caggtagcgc catatccact tgaggcggtg ccatatcaac tattcgccaa
    32461 gatgattggc cgcaaagaat ccaccgtcag aaccatgatt gacgccgcaa agctaccaac
    32521 aattgacttt gtgaaaccgg gttcagtgaa aactcgtgca tcagaaaact gggtatatct
    32581 gccagcattt aacgcaggta tgcgaaaagc attttttaat caacccaaag aacaacgcga
    32641 tgcatggctg ttatggctgg gtctgtaacg atatatccag gaatttttca aaaccaatat
    32701 atctactctg agagagttgt ttgggcataa aaaagcacag gataactaag ctgataaccc
    32761 actacagaca caaaaggaaa cagccatgac tacacaaaaa gcaaaaacca taattaaacg
    32821 atacaacgta cgtagtggtg tggctggcgt tgtcttttcc gatgtgacac agcgacgcag
    32881 cttccgcact ggcacagaac gccacgcagc ccgtttctca accagtgcat cacgcagcaa
    32941 cacgcactac agcctgcagg aaacgcacgc cacgcctgac gggcatctgg ttaaacaaac
    33001 cggtaaacac acctggctga ttaatgatgc cggaattgtc atacacagag gcgcacgcaa
    33061 cccgattacc ggaaatacct gttactccct gactcgcgga gatgagcagt tcgggcagga
    33121 tttcaccctg cacgaagcat taagaacggc agaccggtta atcagcggtc gcagttttca
    33181 gcacccggaa catcgccact gataaataaa aggattcagc catgatcgaa gcctacaaaa
    33241 tacttattaa taatgcgttg cagcgttatc acttcgaact cgcaaaccgg ggtgtaaccc
    33301 gggacgaagg acttcgtaac tcactgcggg gcgcaattat ggggctgtat aactgcgcac
    33361 tgggcagcga tgaccgcgaa gcactggaag aattaaaagt tattgtggcc gaactgtttg
    33421 aaggtgaaat cgttgagcct tatttcacag aggtagcagc atgagcgtat ttctttcatg
    33481 ggtgattctg attatttccg tgcttatggc gatt
//
LOCUS       DAZGTJ010000051        33404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000051 DAZGTJ010000000
VERSION     DAZGTJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.051"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..244
                     /locus_tag="ACHK8E_002947"
     CDS             <1..244
                     /locus_tag="ACHK8E_002947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345184.1"
                     /translation="VTFSNEGIEEVAVLTAGTREDNVAPAAARRANASESSSRMHHLL
                     QKISFKNISFLNNYFIHFLNILLLLHQRQNQAITLK"
     gene            274..840
                     /gene="yqaB"
                     /locus_tag="ACHK8E_002948"
     CDS             274..840
                     /gene="yqaB"
                     /locus_tag="ACHK8E_002948"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP0345185.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            837..1265
                     /gene="yqaA"
                     /locus_tag="ACHK8E_002949"
     CDS             837..1265
                     /gene="yqaA"
                     /locus_tag="ACHK8E_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP0345186.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1338..2894
                     /gene="gshA"
                     /locus_tag="ACHK8E_002950"
     CDS             1338..2894
                     /gene="gshA"
                     /locus_tag="ACHK8E_002950"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP0345187.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3044..3559
                     /gene="luxS"
                     /locus_tag="ACHK8E_002951"
     CDS             3044..3559
                     /gene="luxS"
                     /locus_tag="ACHK8E_002951"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP0345188.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3610..4722)
                     /locus_tag="ACHK8E_002952"
     CDS             complement(3610..4722)
                     /locus_tag="ACHK8E_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0345189.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4719..5426)
                     /locus_tag="ACHK8E_002953"
     CDS             complement(4719..5426)
                     /locus_tag="ACHK8E_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFP0345190.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(5684..7222)
                     /gene="emrB"
                     /locus_tag="ACHK8E_002954"
     CDS             complement(5684..7222)
                     /gene="emrB"
                     /locus_tag="ACHK8E_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP0345191.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7239..8411)
                     /gene="emrA"
                     /locus_tag="ACHK8E_002955"
     CDS             complement(7239..8411)
                     /gene="emrA"
                     /locus_tag="ACHK8E_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP0345192.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8538..9068)
                     /gene="emrR"
                     /locus_tag="ACHK8E_002956"
     CDS             complement(8538..9068)
                     /gene="emrR"
                     /locus_tag="ACHK8E_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP0345193.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(9159..9494)
                     /gene="ygaH"
                     /locus_tag="ACHK8E_002957"
     CDS             complement(9159..9494)
                     /gene="ygaH"
                     /locus_tag="ACHK8E_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP0345194.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9484..10221)
                     /gene="ygaZ"
                     /locus_tag="ACHK8E_002958"
     CDS             complement(9484..10221)
                     /gene="ygaZ"
                     /locus_tag="ACHK8E_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP0345195.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10345..11529)
                     /locus_tag="ACHK8E_002959"
     CDS             complement(10345..11529)
                     /locus_tag="ACHK8E_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345196.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(11721..12713)
                     /gene="proX"
                     /locus_tag="ACHK8E_002960"
     CDS             complement(11721..12713)
                     /gene="proX"
                     /locus_tag="ACHK8E_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP0345197.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12771..13835)
                     /gene="proW"
                     /locus_tag="ACHK8E_002961"
     CDS             complement(12771..13835)
                     /gene="proW"
                     /locus_tag="ACHK8E_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP0345198.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13828..15030)
                     /gene="proV"
                     /locus_tag="ACHK8E_002962"
     CDS             complement(13828..15030)
                     /gene="proV"
                     /locus_tag="ACHK8E_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP0345199.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(15386..16345)
                     /gene="nrdF"
                     /locus_tag="ACHK8E_002963"
     CDS             complement(15386..16345)
                     /gene="nrdF"
                     /locus_tag="ACHK8E_002963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0345200.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(16355..18499)
                     /gene="nrdE"
                     /locus_tag="ACHK8E_002964"
     CDS             complement(16355..18499)
                     /gene="nrdE"
                     /locus_tag="ACHK8E_002964"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0345201.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(18472..18882)
                     /gene="nrdI"
                     /locus_tag="ACHK8E_002965"
     CDS             complement(18472..18882)
                     /gene="nrdI"
                     /locus_tag="ACHK8E_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP0345202.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(18879..19124)
                     /gene="nrdH"
                     /locus_tag="ACHK8E_002966"
     CDS             complement(18879..19124)
                     /gene="nrdH"
                     /locus_tag="ACHK8E_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP0345203.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(19371..19700)
                     /gene="ygaM"
                     /locus_tag="ACHK8E_002967"
     CDS             complement(19371..19700)
                     /gene="ygaM"
                     /locus_tag="ACHK8E_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP0345204.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            19852..20196
                     /gene="ygaC"
                     /locus_tag="ACHK8E_002968"
     CDS             19852..20196
                     /gene="ygaC"
                     /locus_tag="ACHK8E_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP0345205.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(20233..20682)
                     /gene="alaE"
                     /locus_tag="ACHK8E_002969"
     CDS             complement(20233..20682)
                     /gene="alaE"
                     /locus_tag="ACHK8E_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP0345206.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            21349..21753
                     /gene="stpA"
                     /locus_tag="ACHK8E_002970"
     CDS             21349..21753
                     /gene="stpA"
                     /locus_tag="ACHK8E_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP0345207.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(21800..22324)
                     /gene="ygaP"
                     /locus_tag="ACHK8E_002971"
     CDS             complement(21800..22324)
                     /gene="ygaP"
                     /locus_tag="ACHK8E_002971"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP0345208.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(22334..22633)
                     /gene="ygaV"
                     /locus_tag="ACHK8E_002972"
     CDS             complement(22334..22633)
                     /gene="ygaV"
                     /locus_tag="ACHK8E_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0345209.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            22816..22974
                     /gene="yqaE"
                     /locus_tag="ACHK8E_002973"
     CDS             22816..22974
                     /gene="yqaE"
                     /locus_tag="ACHK8E_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0345210.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            23058..23507
                     /gene="kbp"
                     /locus_tag="ACHK8E_002974"
     CDS             23058..23507
                     /gene="kbp"
                     /locus_tag="ACHK8E_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP0345211.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(23508..24170)
                     /gene="csiR"
                     /locus_tag="ACHK8E_002975"
     CDS             complement(23508..24170)
                     /gene="csiR"
                     /locus_tag="ACHK8E_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP0345212.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(24191..25591)
                     /gene="gabP"
                     /locus_tag="ACHK8E_002976"
     CDS             complement(24191..25591)
                     /gene="gabP"
                     /locus_tag="ACHK8E_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP0345213.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            complement(25829..27109)
                     /gene="gabT"
                     /locus_tag="ACHK8E_002977"
     CDS             complement(25829..27109)
                     /gene="gabT"
                     /locus_tag="ACHK8E_002977"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP0345214.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            complement(27123..28571)
                     /gene="gabD"
                     /locus_tag="ACHK8E_002978"
     CDS             complement(27123..28571)
                     /gene="gabD"
                     /locus_tag="ACHK8E_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0345215.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(28594..29862)
                     /gene="lhgO"
                     /locus_tag="ACHK8E_002979"
     CDS             complement(28594..29862)
                     /gene="lhgO"
                     /locus_tag="ACHK8E_002979"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP0345216.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(29882..30859)
                     /gene="glaH"
                     /locus_tag="ACHK8E_002980"
     CDS             complement(29882..30859)
                     /gene="glaH"
                     /locus_tag="ACHK8E_002980"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP0345217.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            complement(31196..>33404)
                     /locus_tag="ACHK8E_002981"
     CDS             complement(31196..>33404)
                     /locus_tag="ACHK8E_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP0345218.1"
                     /translation="PTLLSWNITDGDLNSKLNTLEYLNCITNIINACGVYPQDLKDRE
                     IISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSITAPDRIGEGKTWVYQRDT
                     MVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPS
                     GQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIREN
                     MSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFI
                     ALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQ
                     VLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDEWENLHALKEKRLNGMPKT
                     TPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLTIYNERLHNTN
                     LFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGD
                     LQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALP
                     FSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGI
                     WSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNM
                     GSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPPNYLYL"
BASE COUNT         8269 a         8476 c         8513 g         8146 t
ORIGIN      
        1 cgtaaccttc agcaatgaag gtatcgaaga ggtagcggta ttaaccgcag gcaccaggga
       61 ggataacgtt gcgccagcag cggcccgaag ggcgaacgcc agtgagtcat cctcccggat
      121 gcaccatctt ctccaaaaaa tttccttcaa aaatatctca tttctaaata actactttat
      181 ccatttcctg aatattcttc tccttctcca tcagcgacaa aatcaagcaa ttacgctaaa
      241 gtagcgtcat atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt
      301 tttgatatgg atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa
      361 gtattagggc actacggtct tcagtacgat gttcaggcga tgattgcgct taatggctcg
      421 cccacctggc gtattgctca ggcgattatt gagctgaatc aggccgatct cgacccgcat
      481 gcgttagcgc gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg
      541 cttcctcttg ttgaagtggt gaaaagctgg catggtcgtc gcccaatggc tgtaggaacg
      601 gggagtgaaa gcgccatcgc tgaggcattg ctggcacacc tgggattacg ccgttatttt
      661 gacgccgtcg tcgctgccga tcacgtcaaa caccataaac ccgcgccaga cacatttttg
      721 ttgtgcgcgc agcgtatggg cgtgcaaccg acgcagtgtg tggtcttcga agatgccgat
      781 ttcggtattc aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga
      841 gtgaagcgtt atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac
      901 ccggcaactc cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt catccctggg
      961 ttttagtttt aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg
     1021 ggcgtttctt tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga
     1081 aacgctatgg tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt
     1141 gtctgttagc gggatggatg cgcatctcgt ggggaccggt aatctttttt ttgtgccttg
     1201 gtaaagcgtt acgctatgtt gcggttgcag cagctaccgt tcagggcatg atgtggtggc
     1261 actaattgta ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg
     1321 acaggcggga ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac
     1381 atcctcaggc gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg
     1441 ctgatggcac actggcaaca acaggtcatc ctgaagcatt aggttctgca ctgacccata
     1501 aatggattac taccgatttt gcggaagcat tgctggaatt tattacacca gtggatggtg
     1561 atattgaaca tatgctgacc tttatgcgcg atctgcatcg ctatacggcg cgcaatatgg
     1621 gcgatgagcg gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg
     1681 aactggcaca gtacggcact tctaacaccg gtcgctttaa aacgctgtac cgtgaagggc
     1741 tgaaaaatcg ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactat aatttctctt
     1801 tgccaatggc attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga
     1861 aaatttctgc gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc
     1921 cttatctgtt tggtgcgtct ccagcgattt gttcttcttt cttgcaagga aaaccaacgt
     1981 cgctgccgtt tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc
     2041 gtttgagcga tctcggctat accaataaat cgcaaagcaa tcttggtatc accttcaatg
     2101 atctctacga gtacgtggcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg
     2161 cgaagattgg tattgagaaa gacggtaagc ggctgcaaat caacagcaat gtgctgcaga
     2221 ttgaaaacga actgtacgcg ccgattcgtc caaaacgcgt tacccgcagt ggtgagtcgc
     2281 cttctgatgc gctgttacgt ggcggcattg aatatattga agtccgctcg ctggatatca
     2341 acccgttctc gccgattggt gtagatgaac aacaggtgcg attcctcgac ctgtttatgg
     2401 tctggtgtgc gctggctgac gcaccggaaa tgagcagtag cgaacttgcc tgtacacgcg
     2461 ttaactggaa ccgggtgatc ctcgaaggtc gcaaaccggg cctgacgctg ggtatcggct
     2521 gcgaaactgc gcagttcccg ttaccgcagg tgggtaaaga tctgttccgc gatctgaaac
     2581 gcgtcgcgca aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg
     2641 aactggtcgc ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta
     2701 tgattgatac tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc
     2761 tgcgtgaaga gccgttggaa attctgcgcg aagaggattt tatagccgag cgcgaggcgt
     2821 ctgaacgccg tcagcaggaa atggaagccg ctgataccga accgtttgcg gtgtggctgg
     2881 aaaagcacgc ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg
     2941 aattcaggga tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga
     3001 aagagttcag aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag
     3061 cttcacagtc gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa
     3121 caccccgcat ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga
     3181 agtgatgcca gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa
     3241 ccatcttaac ggtaatggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg
     3301 tttttatatg agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc
     3361 ggcaatggaa gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta
     3421 ccagtgtggc acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat
     3481 tctggaacgt gacgtgcgca tcaacagcaa cgaagaactg gcgctgccga aagagaagtt
     3541 gcaggaactg cacatttagt cagtttaagc agaagaggcc gggctggtga aaacaccgcc
     3601 cggcttttgt tacctgtcag tcatccgata ttccacgcta tgtgcttcat caggttgagg
     3661 aacttccagc cttgaggcca tccgcatcac gaccgcttca ggaactgcat actcgcgtct
     3721 ggcattttgc tgcttccatt gcgcccatgg cacttccaga taaacgattt ttactcgtgc
     3781 gccgtaagcc gtaaacagac tgatgagctg gctgcgcagc tggcgggtaa tgttcgttgc
     3841 attccagatg aacggctttt tctggcgtaa aaaaacgcgc gcctcttctt ttgcctgttg
     3901 cacaatacgc ccggtggcgg ttttgtcgtc cggactggca ttgattcgcc gccgcatatc
     3961 atcaaggctg ataacgtcca ttccctggca ctgttcactg atatagcgat ctttgcccat
     4021 acccggtaag ccacacagca aaataacctc aaaaggttca gcctcccagg gaacaaaatc
     4081 aggagaactt tgctcatgag tcaggtagtg ccagcgtgcg gaatcagaaa cgaacgggcg
     4141 catttttccc cagcattgtt gctcatggca aaacagctcg aacagatcga tgcgttccag
     4201 cattgattgc tgatccgggc tttggcgacc aagcagatcg gcacgagcaa gcaaagcgag
     4261 cagacgcgtg tcgatgcgca ttgcagccgt gagcagcaaa cgctccggtt ccggacgttc
     4321 cagtaaccat agcggtaacc catgcagacg caccagcgca acaatttgct cgcgtagcac
     4381 gaacggcgtt gggatatcgc gccataaaat ttgtcgggcc gtcagttcgc cccgacgagc
     4441 atggccaggc gactgaatac gtccgttttc ctgcacggta gtgctgcgct tttcaacatc
     4501 atgcaataac gccgccgccc ataaaacttc ctgctgttga gcggggagtt gctggaattc
     4561 tggctgagta acgagggcgt tcagcaccat ttcggtatgc acgccgacgt caccttcacc
     4621 atgatgctcc gggtcttgtg gcgtgtgatg catctcctca acccagctaa aacgctgacg
     4681 tagcgccgac cagcgtttgt cgtcggtaag ctgccaaatc atgactgttc tccataatcg
     4741 aactcatggg ccatccgcgc ccgttgccag tgtcgtttcc agtggacggt ggttttgaca
     4801 tgatttttgc gcacatactt gaagacgtta tgcgaaaagt cagcgacgga aaacgcatca
     4861 cgattcctgc tgacaatgcc ttccagcgta cagggttgcc cggtttgcgt atcccaggga
     4921 tcgaacgcgc cgcgcgcatt ggtcagcgcc agaaaatcac gttgccagct tttttcatca
     4981 ttccccggtt gcgggccgga gatttcggga acgcagggga aatcaaacag agcggcgtaa
     5041 aactgcacct cttcccagct cagccacata tcctggcagc gaacggcaaa cagataaaaa
     5101 tcctgctcca gggcgcgata ttcaatcgaa tgcacggcat aaagattttc accaaacaac
     5161 tccagatcgc cgagatcgtt tttcagcagt tgccagcgtt ggcgaatttt ataggtccat
     5221 gccgactgcg taggcgcggc atgggaacgg gcaaacacgc cgtagcggtt aaggcagtta
     5281 ttttcaccgt cgagtttttc tgtgtgcacc aattgcgtga tggcctgtaa atcctgccag
     5341 taatcggcat taatgcggtc atcgctggtg gtgccggggg agaaaggata atgccaggtt
     5401 ctcccgtatt ttcgttgggt attcataata atccattatt ttgtgtgtaa taaatgtacg
     5461 cccgcgaggg tgcgagttat ggggttaaaa gaaaaatgtg agtatcaccc gcgaaagcga
     5521 gggcgaaaga aaaagagaat taacaacctg agcgaatgct cagaaccggt taatggtgaa
     5581 tggaagagtt tggggcggcg aaaagataca ccgaatgagt ggataaaaca accgtttgca
     5641 aaatgagcaa ataaaaaggg agccgcagct ccctttgaca accttagtgc gcaccgcctc
     5701 cgccgccacc tgcaccaaat ggcggtttag caaaccacac cagccccagc aggacgagga
     5761 atattccggc tgacatccag aagatctcat tggcggaaat aatcagcccc tgattagtga
     5821 tctgctgggc aatccagcct gatgcctgtt gttgcgtcat ccccagccct tccagttggc
     5881 tgtacatcgc ctgagcgttc gggttaaacg ggtttaccga ctcggttaac tgtgcgtggt
     5941 gcatcgactc gcggttagtc cacatggtgg tggtgatgga tgtgccgata gaccccgcca
     6001 gcgttcgcgt aaagttggag aggctcgatg ccgccgccag tcgttccggt ggcaaaccgg
     6061 acagcgtaat ggtggtcagc ggcataaaga agcaggccac cgcaaacccc tggataaact
     6121 gcggccaggc cgacgcgcca aaatccatac ctggttcaaa ggtataggcg cgccagtaga
     6181 agcagacggc atacataata aagctgaagg ttaccagccg ccgcatatcc agtttatgcg
     6241 cgaagcggcc gataatcggc gacaggatca ccggaataat ccctaccggc gcagaggcca
     6301 gacctgccca cgttgccgtg taaccgtaga cctcctgcaa caactgcggc agcagaacaa
     6361 tcgcgccgaa gtagagcata tacgcgaggc tgatacacaa gcagccgatg gtgaagttgc
     6421 gcgacttaaa caacgacaga tcgactatcg ggttatcgtc ggtcagctcc cagacaatca
     6481 ggaagcagat agccaccact gccaccacgg taaggatgat aatttcctgt gatgagaacc
     6541 agtccagctc tttaccgcgg tcgagcataa tctgcaggct accgatacca ataaccagca
     6601 gtgccagccc cacggcatca atccgccgcc gttcggtgcg ggtttcacgt ccgcgcaggg
     6661 tttgcagtgt catcaacacc accgccacgc caatcggtac gttgatgaag aatatccagc
     6721 cccagtggta attgtcactg atatacccgc cgaggatcgg gccgcaaatt ggcgcgacaa
     6781 tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt ggctggcggg tagttattaa
     6841 gcaatagact ttgcgaaagc gggatcagcg gcccggcgac aatcccctga atcacgcgga
     6901 agaagatcag catattcagg ctgctggaga caccacacgc ccacgacgca atagcaaagg
     6961 cgatggtgga ccagaggaac agtttcactt ccccgacgcg ctttgccagc cagccggtaa
     7021 gcgggatcga gatggcattc gccaccccga aagaagtgat tacccacgtt ccctggctga
     7081 gcgatgagcc cagattcccg gcgatagtgg ggatcgccac gttagcaatg gtggagtcca
     7141 gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat aatgaccagt tgcgcgcctt
     7201 ccagcggttt ttgctgttgc atcacacgca cctctggatt agccagcgtt agcttttacg
     7261 atatcgtcga tcagtttatt gacaggtgcc aggctgattt cacgcgcggt gcttaccgct
     7321 accggtgtgg aacgtacttt atttgccagt acctgaccgt cacggttagt ggtattgacg
     7381 ctcaccagcg tggacaaacc gatacgcagc gggtattgct cgagctgttt ctggtccagt
     7441 tcaatacgaa caggcagacg ctgaacgact ttgatccagt taccggttgc attttgcgcc
     7501 ggaagcagtg agaacgcgct acctgtgccc atatccagac caaccacttt tccggtgtat
     7561 ttcacatcat cgccgtaaat atcggtggtg atggtgaccg gctgaccgat acgcatattg
     7621 gcaatctgcg tctctttaaa gttggcatcc acccacatat tggtggctgg aacgaccgcc
     7681 atcagcggcg tcgttgggct aatttgcgcc ccaggctgta ccgcgcggcg ggagacataa
     7741 ccggtcatcg gactgacaat acgggtacgc tccagcgcca gccaggcgtt acgtacttcg
     7801 gtggcagcct gttgcacggc aggctgatct tccagtttag tccccagaat catcgcctga
     7861 ttggcattgt attgttgaat agcgacgtcc agttgtgcct gggcactggt aacggcgtcg
     7921 cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat tgcccagcgg cacacggcgg
     7981 ttgtagtcgc tttgtgcttt cgcgagggcg attttttgca cctcaatatt cgcctgcaac
     8041 tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc tggaagccag tgcagttttg
     8101 gctttttcaa acgcctggcg agcatctgtc gggtcgagag tgaccagcac atcgccttct
     8161 tttacaaaat cggtgttatc ggcccagact ttcgtcacgc tgccagacac ctgagacata
     8221 atttgaatct gattccctgc cacgtatgcg tcatcagttt cttcgaaatg acgcagtacc
     8281 aaaaaccaat aaatccctat cgctacggca ataattataa agagcaaggt gagaaggagg
     8341 agcagacgct tacgtttgcc gctcttcttt accggttgct gcggggtttg agtctccgca
     8401 tttgcgctca tattgttctc cacgatcttc ttaactcatc ggctgagccg acctgcttgc
     8461 caaaaaggcc agcatgttgc gatgctggcc agtcattttt tcttttataa atctggattt
     8521 ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca caccgtcttg ttccatctgg
     8581 tcgagacggg agagcaattt gcgggtgatt tgctcgagct gatctttttc tgttgtgctg
     8641 agcgcggacc agagttgatg caggcagtta tgctgcggtg gtaaaacctc gcgcaaaaac
     8701 tcgtgacctt tttccgttaa ttgcagatgc aggcagcggc gatcgttatc gctttcacga
     8761 cgttcgatcc aaccgcgttt ttccagttca tcggcaatac gcgtcgcgtt ggtacgggat
     8821 gatccaagag cacaactcaa ttcagaaggc tgaatactgt ggttttcctg agactccagc
     8881 gtaatcaacg ccataaacaa cgtctcgtta atcccctgag ccttcagcat tttattgcgg
     8941 ttctccagca gcttgctttg catgtgcatg caaagacggg tcagaaggat ctcctgataa
     9001 ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt gttcaatggg cgtaaacgaa
     9061 ctatccattt gggtatgacc tcattaattt cagccgatat agtaacgaca gtgacaaata
     9121 aagtaaatgt attgttttag aaaaatgatt cttgtgggtt atataatcgc catcactttc
     9181 caggcgagcc cataggccag cgcactaagc agtgttggga tgataatgct gcgtgtttta
     9241 tagaaactgg cacccagtac cgcgaagccg accagagtgg gcacgaaacg gcgtgtatcg
     9301 tgcatcactt ctggtgcggt agagacaacc agcagagcgc atatcgaggc gatgccaatg
     9361 gtgtcgagca aaatacctac cgcgccacgt ttggttgggc gggcattacc cacacgcagg
     9421 cgcagcggca aatagcggaa gcaataattc gccgcgccaa ccagtaaccc aagcagcaga
     9481 acctcatagc tcatcgggcg ctccttgcca gaatgcctgg attaacgcag tgaggcagcc
     9541 acagacaatg cctgccagaa tggcgacggg aatagaaaat agcgttacgc ctgcaagggc
     9601 accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc tggaaagagg cgagcaggaa
     9661 actcataaag agtgccggaa gcataaaacc taatgctgct tcaacggcgg gatatccttg
     9721 cagcaagccg ctgccggaga atgcccctat taccgtacca aatacccacg atgaccatga
     9781 actgaaggca atgccgatca tccagttctc gctccagcgg cgattattgc gtaccagttt
     9841 tgcggttgcg gcggcaaaaa cctcatccgt caggccaaac gcccacaggg cagttttcga
     9901 tttttgcaga cgctgaataa tacggctacg cagtgacggg ccatacaaaa catggcgaac
     9961 atccattgcc atgacggtca gtgcagcaac ccacaaacta ctcccggctg ccagcatcgc
    10021 ggtaatgacg aactggctcg cgcctgcata aatgatgcag gagaaaaaaa cgctttcgag
    10081 aggagagaat cccagacggg tcgcattcag accgaacgca aaggccaccg gaatataact
    10141 aataacaatc ggtaaactgt ctttgcatcc ttccatgaag gtcgccgaac caggagcagg
    10201 ctgtggagta gggctttcca tagagtgtac gcttaacgat tgttaatatt ataaatagac
    10261 tgaatgaata tcttaacctt atcagactga tgggcttctt aacaccctta taagtgtaaa
    10321 gccacgaaaa cggttgctga ttgcttaaat tgcggcttcc tgacgatgaa aacctcgcca
    10381 ccagaccagt aagttaacca gggcaatcgt cgcgccagcc agacaaacgc cagcccaacc
    10441 gccatgttgc cagactgagg ctgaaattag cgaaccggcg gcaccgccaa tgaagtagct
    10501 ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg tgtattcgat aaattaccgt
    10561 ctggttagtg atatgcacgc cctgcacggt gagatccagc accaggatgc cgataatcag
    10621 cgccagtacg gaagtgtgac caaaccagat cgccagccat ggaagtaaca gcagcagcag
    10681 accgaaagtt gtggtgtggt gcgatttgcc cttatcggca aatccgcccg ccggacgagc
    10741 gcccaacgct ccggctgctc ccgcaagtcc aaacagacca atgacgccat cgctgtaatt
    10801 aaaaggcgga gcggcgagca aaaaggccat cgaggtccag agaatgctga agttggcaaa
    10861 ggtcaggcag cccagcaacg tgcgggtgcg cagaatttta tcgctgataa acatgctgaa
    10921 aacggaacca agcaactgtg ggtagttgag gtgggtttct gatttcattt gtggcagacc
    10981 acgccataat gccagcgcca tcagtgccat taacaccgaa gcaacccaaa agacggtgcg
    11041 ccagccgccg aggtttgcca gcaacccggc aacggttcgt gccagcaaga tccccaacag
    11101 cagcccgctc ataatggtgc cgaccacttt gccgcgtttg tccggtgaag ccagcgtcgc
    11161 tgccagcgga accagaattt gtgccacgac cgagaataaa ccggttaatg ccgtaccgag
    11221 gatcatcatc gccagcgact gactgctggc agtgattaac atgccgcatg ccgccagtaa
    11281 ggtcatcgag acaatcaggc ggcggcgttc aaacatatca ccgaggggaa caagaaacag
    11341 cagacctgcg gcatagccca actgcgcggc ggtaacaata aagcctgccg aactggcgga
    11401 aagggaaaag ttacgcgcga tggtgtcgag caatggctgg gcgtaatagt tactggctac
    11461 cgccaggccg gtggcgatag acatcagcac gatcagcgcc gggctaagct catgattagg
    11521 tttagtcatt atttttgttc atcaggtgat gattaagctg gaaatcaata atagcggatg
    11581 ttgatgaata tgggatgaaa gatagattgt tgaaatgtgc ggtgtggttt gtaggtatga
    11641 taagacgcac cagcgtcgca tcaggcattg tgtacagagc ggcggatgag gcaaaaacgc
    11701 cttatccgcc cgaataaaaa ttacttctgc gctgccagcg cctcattcac ccagccatcg
    11761 aactgctgct ggtgggcttt gatccagcca tcaacgtggc cctgaatatc gccttctgag
    11821 gctttgccgt catgcataat ggcgttctgg gcgttaatat cagccactgg taactgcata
    11881 atggcaaaca gtttcgctgc tgccgggttt ttctcggccc aggctttgtt ggcaacgata
    11941 tgcatggtac tgaccgggaa gccgtaattc gcgccattcg gcagtttggt atcggcattt
    12001 ttatcgcccg gcagtgcgga gaacggcacc tgcaaccaga ccacatcttt ccccggtttc
    12061 agttcgttac tcacccagta cggcgtccag gtgtagtaaa acaccggctt accctctttg
    12121 taacgactga tggtgtcagc catcatcgct gcgtagttcc cctgattatg cgtcacggta
    12181 ttggtcagtc catacgcggc aagctggtgg ttgatcgcac cttcgcagcc ccagcctggg
    12241 ttacagccgg ttaaatccgc tttaccgtcg ccgttggtat cgaacagttt ggcgatcttc
    12301 ggatctttaa gttgcgcgat gttggtaatt ttgtactggt cggcagtttt cttatcgatc
    12361 aggtaaccct gtgccgcgcc gttaacaaat actccttcac gataaaattt cttatcgcca
    12421 ccggcagctt cgtacatgtt gtcatgcagt ggcgtccagt tcactgcggt gaaggttgca
    12481 tcgccggaag caagcgaggt gtagccaacg ttgtaatcca cttcgctggg tttattgacg
    12541 gtataaccta atttctccag cgcgcggctg accagcagcg tctggaaagt ttcttcggtg
    12601 atggtgctct gaaccggatt aacagtaatg cctttgcccg gcaggtcggc agcaaaagtt
    12661 tgtgtagaga taagcgtggc aaacgctgtc gcaaaaagta cgctatgtcg cattgttatt
    12721 cctttttttg gcagggtgat aatgcccggc gtcaccgggc aagtgcagag ttacttaatg
    12781 aatgggcggg tcagcagacc gacagggcca gtggtgtacc agcgacggtt gccgcgactg
    12841 cgtgagtcgc gcccaacggc ctgcgtcagg cggtcgagga taatggcgag gatcacaatc
    12901 ccgacgccgc caacggtggc aagccccata tccagacgac cgataccgcg aagcaccatc
    12961 tgacccagcc cgccgacggc aatcatcgag gcgatgacca ccatagaaag ggccagcatc
    13021 agcgtctggt taacgcccgc cataatggtc ggcatcgcca gcggtaactg aactttgaac
    13081 agcatctggc gcgggctggc accgaatgag cgcgaggctt caatcagatc cgccggaacc
    13141 tggttaattc ccagaatcgt cagacggata atcggcggca gcgcaaagat gatcgtcacc
    13201 accacgcccg gcacgttacc gataccaaac agcatgacga ttggtaccag ataaacaaac
    13261 gcgggcgtgg tctgcatggc atcaagcagt ggacgaataa ttttcgccgc tcgcggactt
    13321 ctcgccagcc agatccccaa cggcaaaccg atgacgatac agaacagcag ggcggttagc
    13381 accagcgcca gagtaaccat tgcctgtgac caggcaccga ttgcgccgat ggcaatcagc
    13441 gaaaccagtg tcgccacgcc cattccgaca ccggaaattt gccaggcgat gagagcgaaa
    13501 acgataatcg ccaccggagc gggcataccc agcagcaatt gctggaaacc gttgaggatg
    13561 taatcaaccg gaacgcgcac gccctgaaag acgggacgaa aatgggtaac gacccagtcg
    13621 atcccttcag tgacccaact gtcgagcggg atcagcgttt tatggaacgg atcgagaata
    13681 ttaaaatgct cgacgtttgg cgcaggcgta ctggtcagcc agtcagcacc accgccgtca
    13741 gtcggtgcag cagccggtgt accccaggcg tctgcggact gtgcggcact gtccgccgct
    13801 ggcgtggtat cccacggatt attttgatca gccattattt accccctcac gatctaaagc
    13861 gcgcagcagc attcctttcg aaatgatgcc gacatactgt tggtcctcat cgaccacggg
    13921 caccgcacag ggggcctgtc cgacatgaga gagcaactcg ctaagaggcg tttgtgcatc
    13981 gaccgctaac ggcgcatcaa tcagcgccgc atcaagacct tgctgctgcg ttaacgcggc
    14041 tttaagcgaa tcgatagaca cagcgccgac aaacttatta ccgcgttcga tgacgtagcc
    14101 atattcgcga tcttcatcct gcaataattt cagtgccgaa cgcgggccga agccgggggt
    14161 tttacgaatt aagccgttcg gtgtacgacg ggcaatatct ttcgcactga acacctggct
    14221 aatatcgacg ccacggaaga aggtacggac ataatcattc gccggattat tgagaatttc
    14281 atccggtgtg ccgacctgta ctacttcacc attttgcata atggcaattc ggtcgccaat
    14341 acgcatggct tcatcgagat cgtgggaaat aaagacaatg gtgcgctgat gtttcgcctg
    14401 taattttacc agctcatcct gcatctcggt gcgaattaat ggatcgagcg ccgagaaggc
    14461 ttcgtccatt aataatatat ccggattaat cgctaacgcg cgggctaatc ccacacgttg
    14521 acgcatcccg ccagagagtt catccgggta gctgtgggca taattttcca gcccgacctg
    14581 acgcagtgca tcaagggctt tttcacggcg ttcttcggca ttaattccgg ccagttccat
    14641 accgaacgca gtattgtcca gcacggtcat atgcggcatt aaggcaaagg actggaagac
    14701 catcgcaatc ttttttctgc gcacctcacg gagttcggcg tcggatattt tggcaatatc
    14761 cacaccatca atcagcactt gcccgcgggt gggttcaatc aggcgattga gaaggcgtac
    14821 cattgtggat ttacccgaac cggataatcc catgatgaca aatatctcgc cttcttcaat
    14881 ggccagactg gcgtctttaa cgccaagcga tagcccagtt ttttccagaa tttgttcttt
    14941 tgaaagtcct tgttcgatat atttgaaagc tcgctgtgga tgctcgccaa atattttata
    15001 aagattttta atttctaatt taattgccat gcaatagaaa gattcctgtt atttgtttat
    15061 gtcgatacat tacccaatgg aaatagtcac ttttttctac cctaacatac tgagaatctg
    15121 aggcaacccc tgatggcaaa tgtggcatga atattgatgt gagcaaatcg ccggaatttc
    15181 ccatgatata agggctgaga gcaaatcgag aaaagttgat attttttgtc aaagatagca
    15241 aaaaaacgcc tgaatattgt ggtttcaggc gaataatgca acagagtatc agtgtagatc
    15301 accacaaatt atttgcgtga tataaagcta tttggcggga attatttccc tgcagcgggt
    15361 agtgatattt ttgaaaataa ccccctcaga aattccagtc ttcatcttct gtttcgaccg
    15421 cttttcccat cacatatgaa gagcctgaac cggaaaagaa atcgtgattt tcatcggcat
    15481 tcggcgaaag cgcggcgagg attgccggat tcacttccgc catttctgcg ggaaatagcg
    15541 gctcgtagcc cagattcatc aacgccttat tggcgttgta acagagaaac gctttcacat
    15601 cgtcagccca cggggtttcg gcgtacagct catcggtgta ttgcaactcg ttgtcataga
    15661 gttccagcaa caaatcgaag gcgaaactct tcaattcttc acgtcgtgcc tgggagatct
    15721 tttccaggtt tttctgatat ttatagccga tgtagtaacc gtggactgct tcatcgcgga
    15781 taatcagacg gatcaggtct gcggtattgg ttagctttcc gcggctggaa aaatacatcg
    15841 gcagccagaa accggaatag aacaaaaaag attcaagaaa cacactggcg attttctttt
    15901 tcagcggatc atcaccgcga taatgttgct gaataatctg tgcttttcgc tgcaacggcg
    15961 cgttttcttc actccaggcg taggcggcat cgacatcttt ggtctggcac agcgtcgaga
    16021 aaatcgaact gtaagagcgg gcatgaaccg cctccataaa gctgatattc gataatactg
    16081 cctcttcatg aggcgtgagc gcatcgggca tcagcgaagg cgcgccgata acattttgca
    16141 gcgtgtcgag cagtgtcagg ccagtaaaaa cgcgcatcgt cagttgttgt tctacgacac
    16201 ttaatgtctg ccaggcggga atatcgttcg atagtggcac cttttctggt agccagaaat
    16261 tgctggtcag gcggttccac acctccagat ctttatcgtc tgttatcttg ttccagttga
    16321 tggcgctgat acgtgagagt ttcatagata ttccttaaag tgcacaggag acgcagcctt
    16381 caatttcagt gccttccagc gccatctgac gcaggcggat gtagtagagc gttttgatac
    16441 ctttgcgcca ggcgtaaatc tgcgctttgt tgatatcgcg agttgtggcg gtatcgggga
    16501 aaaacagtgt cagcgacagc ccctgatcga catggcgagt cgcttcggcg taggtgtcga
    16561 tgatcttttc tgcgcctatt tcgtaagcgt cctgatacag cgccagattc tcgttagtca
    16621 taaacggggc aggatagtaa acgcgtcctg ttttgccctc tttgcgtatc tctactttcg
    16681 ccacaatcgg atgaatactc gacgtggcat ggttgatata agagatagaa ccggttggcg
    16741 gcaccgcctg gagattttgg ttgtatatgc catagcgcat cacgtcgtcg cgcagttgcg
    16801 cccacatttc gcgggtaggt aacgtgatgc tgctacgagc aaacaactcg ccaactttcg
    16861 ccgttttcgg ttgccagtta ctttgcagat attggctaaa atattcaccg ctggcatagc
    16921 gcgactgttt gaacccggca aaggtttcgc cgcgttcgcg cgccagcaac atcgaggtac
    16981 gcagtgcgtg ccaggtgatg gtatagaaat agagattggt gaaatcgagt gcttccggcg
    17041 atccataagc gatgccttct cgcgccagat aaccgtgtaa attcatctgc cccagtccga
    17101 tggcgtgtga ggcggcattt ccggcttcga tggacggcac gctgcggata tgactcatat
    17161 ctgacaccgc cgttaaaccg cgcacggcag tctctaccgt gcgggcaaag tcgggggaat
    17221 ccatggtgtg ggcaatattc aacgaaccta aattgcagga aatatcatgg cctgtgcggg
    17281 catagtcgag attctcgtca tactctgagg cgctgttaac ctgcaaaatt tctgagcaga
    17341 gattgctcat atttatgcgc ccggcgatag ggttagcacg gttaaccgtg tcttcataca
    17401 tgatgtacgg atagccggac tcaaactgga tttctgccag tcgctggaag aaatcacggg
    17461 cgttgatgta ttttttgcgg atgcgttcat tggcaaccag ttcggcatag tgttcactga
    17521 tggcgacatc ggcaaatggc ttgccataaa ctcgctctat gtcatagggc gaaaacagcg
    17581 ccatctgcgc attctctttt gccagatgga aagtgatatc cgggatcacc acgcccagcg
    17641 acagtgtttt aatgcggatt ttttcgtcgg cattttcccg ttttgtatcg agaaaacgca
    17701 gaatatcggg atgatgagca tgtaaataga ctgcgccagc accctgacgc gcgccgagct
    17761 ggttggcata ggaaaatgcg tcttccagca ttttcatcac cggaatcacg ccagaagatt
    17821 gattttcaat acgtttaatt ggcgcgcccg cttcccgcag attcgacagc aaaaatgcaa
    17881 cgccgccgcc gcgtttcgac agttgcagtg cggaatttac tgcccggcca atcgactcca
    17941 tattgtcttc aatacgcagc aaaaaacagg aaaccagttc gccgcgctgc tgcttaccac
    18001 agttgaggaa tgtcggtgtt gcaggctgaa agcgtcctga cagcatctca tcggtcagtt
    18061 gcgtcgccag tgtttcatcg ccttgtgcca gcgtcagcgc caccatcgtg actcgatcgg
    18121 caaaatcttc cagataacgc ttaccgtcga atgtcttcaa cgtatagctg gtgtaaaact
    18181 tccatgctcc gaggaatgtc tgaaaacgaa aaccgctggc gtgtgcgtgg gcaaacagcg
    18241 taatgacaaa atcgctagag tagcgattaa ggacactttc atcatagtaa ccttcgttaa
    18301 ccagccagtt caggcgctgc tgctggctac tgaaggccac actgttcgga cgcacatgcg
    18361 tcgtcataaa ggcgtctact gcctggcgat ctttatcgaa ctgaatgcga cctgcgctat
    18421 cgtaaaggtt aagcatcgca ttcagcgcgt ggtaatccat agtttcctgc gtcaggcgtt
    18481 ctgcggttgt cgttgccaaa attcggttac tcctttacga acgttttcga tatcgctttg
    18541 cgtacccatg agttcaaagc ggtacagcca tggcacgccg catttccggg caatcacatc
    18601 tccggcgcgg ccatatgcct caccaaagtt gcgattaccc gaagcaataa cgccgcgcag
    18661 caacgcccgg ttatgctcgt cgtttaaaaa gcgaatgacc tgtcgtggca ccgcgccagc
    18721 cgtaccgccg ccgccgtaag agggcacgat caggatgtaa ggctcgtcta cctgaatccg
    18781 ttcccgctca ttgagcggga tgcgcaccgc gggcagccct aaacgttcga taaaacgctg
    18841 cgtattttcg gagctgctgg aaaagtagac aatctggctc atgcattggc cgcgtgtggt
    18901 gctggatgca gacggttgat catgtccggg cggaaacctg accagctaag atcgcctgca
    18961 atcacgaccg gcaactgacg aaagccctga gcacgcagcg cttccgccgc ttcaggaacg
    19021 cgatcgacat taatcatttc aaagtcaaag ccccggtttt ccatcgccct tttggtggcg
    19081 tggcactgaa cgcaatcgtt acgagtgtaa atagtaatgc gcatgattcg tatttccgtt
    19141 taaaatgaag atacggcgcg atgatacgcg tcgggttgtc tctctgttga tacagaaata
    19201 ctagatgtag ttgaaaaaag attcaaccac acaatatata gcaaatagtg gtcgaaatta
    19261 ccctgggtat gagcgtgacg gggtaggggg atttttgtga ttcaccaggc aaaaagaaac
    19321 cccgaagcca ggcttcgggg ttaaagacgc gtatttatta tcatgttgca ctacgatttg
    19381 cgcatgctta acagtgcgcc gataaaaata cctaccgcag ctgctgtacc cacgctacac
    19441 cagggtcttt cacgaacaaa agaatccgcg cagccaacgg catcgcgcgc ggcttgctgg
    19501 acgcgagtac gaccatgcat tcgcgctctg gtttctttca gtagagcctg cgctttacta
    19561 cgagcggctt cggcttcccc tttggcgtcg ctgccccagg acttcaatac agattcgaga
    19621 ctatcagcta attgattgac atcattctgg atatcctgca cgccatcatc aacgtcgttg
    19681 cggttcggtc ggttaaacat atgatccccc attagaattc gatgtaattt tcagtgtaga
    19741 ccataatttt cttaactgag gttcatcgca gctttatgga cttttctgaa agcgttgtga
    19801 atgcgcaatc aggtaaggta ggcccgcggt tgataataac gaggatcaaa tatgtattta
    19861 cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga tgtggtaacc
    19921 cagaaggctt atggttaccg ccgtggggaa aattatgtct atgttaatcg cgaagcgcga
    19981 atgggacgta ccgccctggt gattcatcca acattaaaag aacgtagttc aacactggct
    20041 gaacccgctt ccgatattaa aacttgcgat cattatcaac aatttccgct ctatttagca
    20101 ggcgagcgac acgagcatta cggtatcccg catggcttta gttcgcgtgt tgcgcttgaa
    20161 cgttatttga atggtttatt tggcgaagcc agttaaagac gcgactggcg atgccagtcg
    20221 cgaaaagaag ggtcaggctt ttacctgctg gtaacggctg actttaaaca gtcggcggca
    20281 ataatcgagg aagtagccat aaaccgcccc catcaacatc gaaacaacga tgtttgaact
    20341 gaccgccgcc ataatctgat gccagtctgc gcccaccact aacaagatcg ccacatacac
    20401 cggtgactgg aacgtcacat aagccatgat atccgccaga ttttttatcc agcccgacgg
    20461 gctaactttg cgtgctgcgc gcataaacag atcgcggtac ataccgtatg gccatgcaat
    20521 taagatgttt accggaatcg ctaccaatct ggaataaaaa gactgttcga agctcattcc
    20581 ggagaggaaa acttcaatac acatgttcac gacagaacag taaacaacca tcgcgaacgt
    20641 atctgcaact gcatgacgca agcgtgactg cggtgagaac atgctaatgc tccttaataa
    20701 aagcgtaatg ggatgtgaat ggagatgaaa gcagtggatc tcattgccat ctgcattttt
    20761 gcatagcgta tatcgctggc tatgactaat caattagtga aaaattttta tttaatctgt
    20821 ttttgtagat aaaatattcc gtagcggcgc gttttttgta cattgtggat ttttacatgc
    20881 cgatatgatt tatttttaat taaatcaatt ggatatgtgt ttacctttat tttcactcat
    20941 gaaggtttgg gggggggtaa cgctataagc gtagagaatt cggtgtatgt agtgatcggg
    21001 tcattttcag cattttttat gccagataat gtacattgac agcagaaagc accagtgata
    21061 gatgttttct tttattctgt tgagttgcat ttttattcac tttggttttg tcgggcttgc
    21121 ggaattagcg agcagagagc gccgctctgt tcaccaataa cctaaattac tcatcaaaat
    21181 ggctaatata taatcttgaa attattctga gggtttcaga aaataacagc tgaaataatc
    21241 tcgtgcagga ctgtaaatag attaaatttt gtggaaatat aataagtgat cgcttacact
    21301 acgcgacgaa atactttttt gttttggcgt taaaaggttt tctttattat gtccgtaatg
    21361 ttacaaagtt taaataacat tcgcaccctc cgtgcgatgg ctcgcgaatt ctccattgac
    21421 gttcttgaag aaatgctcga aaaattcagg gttgtcacta aagaaagacg tgaagaagaa
    21481 gaacaacagc agcgtgaact ggcagagcgc caggaaaaaa ttagcacctg gctggagctg
    21541 atgaaagctg acggaattaa cccggaagag ttattgggta atagctctgc tgcggcacca
    21601 cgcgctggta aaaaacgcca gccgcgtccg gcgaaatata aattcatcga tgttaacggt
    21661 gaaactaaaa cctggaccgg tcagggccgt acaccgaagc caatcgctca ggcgctggca
    21721 gaaggtaaat ctctcgacga ttttctgatc taattactga ggccggatgt tgctgctatg
    21781 gcgcgtccgg cgtcgcttct caagctcgtt ggttccacgg catcttatct aacaaccttg
    21841 ccatcccaca aaaaccgcta attcctgcaa acagtaaccc ggcaccaaca aagccgctta
    21901 ataagaagaa accgctattt acggtataac ctagtataac gccgattaat attaaaccac
    21961 ccgcagcgat ctgcacctgg cgcattaacg gcaagggttg agatttattt accgctactg
    22021 gtaatcctgc ttttttccag ccatcaatcc catcttcgag taaaaagatt tctgcggggg
    22081 cggcaatcgc tgctaattta tcggcgttat tactggtgcg tttaccagcc tggcagtgga
    22141 atattatttg ctcgcgacgt aatttagccg gaagacctga ctgttccagc acggataatg
    22201 gagccagatc tgcttcagga atatgttcac gaagatattc atcagcgtca cgaatatcga
    22261 ttaactttgc gccgcgtgcg attaattctt gtgcatcatg cggcgaaatg gttgtcaaag
    22321 ccatagtgac tccttacgga caatagacat ttttcagggt ggcgataatg gcatttaccg
    22381 cctcattttt aatggaatat agaatgcgtt gggcatcccg ttggctgtcg ataagccctt
    22441 cgtcccgcat acgagcgaga tgctgtgatg ttgcagaggc actcagtccg gtaatgcgcg
    22501 tcagctctcc cgcactggtg ccgggggaac cgctaagcat gcacagaatc agcaaccgtt
    22561 tagggtggct cattgctttc aataaggccg ctgcctgttc ggcactagcc tgtaattgcg
    22621 cgagttcagt catagatatt taagtatttt ctaaattaag taaactctaa actaaaaatg
    22681 caacatatac cagcctcagc agcgtaaatg agagtaaaag cgtaagctga aactggcagg
    22741 ctccgctaaa attactacgc ttaagagata aaatctcttt ttaaacaatg agtaattttc
    22801 ttatagggag tacatatggg tttctggaga atcgtcatca ccatcattct gccgccgctc
    22861 ggcgtgctgc tcggtaaagg gttcggttgg gcgttcatta ttaatattct gctgacactg
    22921 cttgggtata tccccggttt gattcacgcg ttctgggtgc aaacccgcga ttaaacctgt
    22981 gttcaccccc gcggcgttaa cgcgccgcgt tcctctgcta cactttctga gtgtttccgt
    23041 taacgaatga ggtagctatg ggtctgttca attttgtgaa agatgccgga gaaaaactct
    23101 gggacgcggt tacaggtcag cacgataaag acgatcaggc gaagaaggtg caggagcatc
    23161 tgaacaaaac cggaatacct gatgccgata aagtgaacat tcaaatcgcc gacggcaaag
    23221 cgacggtcac gggtgacggc ttaagtcagg aggcgaagga gaaaatcctt gttgcggtgg
    23281 ggaatatttc cggtattgcc agtgtcgatg atcaggtgaa aacggcgaca ccagccgctg
    23341 ccagccagtt ttataccgtt aagtctggcg acactctgag tgccatttcc aaacaggtct
    23401 acggtaacgc taatctgtac aataaaatct tcgaagcgaa taaaccgatg ctcaaaagcc
    23461 cggataaaat ttacccgggg caagtgttgc gtattcctga agagtagtta attgccagcc
    23521 atcgcctgct ggataatggg aattggcgtc agtaaatgct ggcgcattaa ctcactggcg
    23581 cgcgcggtat ctcgcgccag taccgccgca gtgagggtct ggtgctgatc gcgtttatct
    23641 tccagcattt ccaccgaaag cactgttcgc cgcagccaga taaatcgata acgcgccgcc
    23701 agatcaaaca accgttcacg catttgcagc aaatagtgag aaccacagcc cgccacaatt
    23761 gcagtatgaa acgcctgatg acgcagatcc cactcatcaa gcattttctc actggcgtca
    23821 caggcctcca gcttactgag cagatgcgct tttgcgagaa cgtctgcctc ccattcatcg
    23881 ccaccgcggg caatcgccag actgaccagc atcgcttcca tattggcgcg ggcgtcgaaa
    23941 atatcgagca gctcttgctc tgacatagac gccacccgat accctttttg attcaccacc
    24001 gtgaccagtc gttctgctac cagttgcgaa agcgcttccc gcaacggccc aacgccaagt
    24061 gcataacgcg atgtcagcaa actcatccgt aatttttcat ccggttgaaa gtttccgcga
    24121 ataatatcgt tcttcagcca gcgatagcca tccagagacg taatggtcat ggtgtgactc
    24181 ctgatcatta ttagcgcgta ttatgaacgg gtgttttttg ccacaatacc agctttttcc
    24241 agcgagccat aattggcacg gtacagatta tccctatcgc taataaaccg gtggaaatca
    24301 cttctaactg ttgcgccggg cggaatagca tcactaccaa cacaaaggtt ataaaaccga
    24361 tgaccagcca ggtgagccac ggataaagcc acatccgcag gcgaatttcg cttccttctg
    24421 ctcgcagaat tttgcgcatc cgcaattgtg aaacggcaat gactaaataa accagcaggg
    24481 cgatagcgcc ggagctatcg atcagaaatt taaacacttt cgcaggcgcg taataattca
    24541 ccaccaccgt taaaaatgcc gcaccggtcg agagtaatac cgccacatac ggggttttac
    24601 tgcggttgat tttacccatt accgcgggag catcaccgcg acggcttaag gagtagagca
    24661 ttctggaagc ggtatacagc gccgagttca ggcaactggt tacggaaagt aatatcacgc
    24721 agtccatgat taatttcgca tggggaatat tgagcaactc cagaaccgag cgataagatc
    24781 caacggcttt cagccccggc atattccacg gtattaatgc caccacgaca aaaatagagc
    24841 ataaatagaa gatagaaata cgccagataa ccgagttggt ggcgcggaca atatgttttt
    24901 ccggcgtgtc agattccgcg gcggcaatgg tgacaatttc tgcgcccata aacgagaaca
    24961 tggtgatcaa catcgcgctt aatactgcgc cgaagccgtt gggcataaag ccgccgctat
    25021 cccacaatct tgagatcccg ctcacttcgg cataagggta aaaaccgcta attgcgactg
    25081 caccaaggaa aataaaggcc aggatagcga tgactttgca cagcgccagc cagaactcaa
    25141 attcgccgta gtttttaacg cttaataaat tactgccagt caaggcgagg gtaatgacca
    25201 gggaaaataa ccagatggga atgcctggaa cccacgagtg caggatcatg gcggcgatgt
    25261 tggcttccag cgggataacc agtacccaaa accaccagta cagccagccg atggtataac
    25321 ccgcccagcg gccaattgcc ttgtcggcat aggtggaaaa cgaaccggta tcgggcgtgg
    25381 caaccgccat ttccgccaac atccgcataa tcataaccac cagtaatccg gcgaacagat
    25441 aggccagtaa taccgccggg cccgcttcgg cgatggcgac gctggaaccg acaaacagac
    25501 ttgcgccgat aacaccggca atagacaaca tggtgacgtg gcgtgacttc agcccgccgc
    25561 ctaactcatg tggttgcgat gattgcccca tcctgaatcc tctcgaaagt gtgtcacttg
    25621 ggagagcgcc ccgacatttc ccgcgtcggg tcacgcgtgt aattgttatt ggttcttttt
    25681 accggagagc ggctccgcct tatccggccg acatagattt attcctgttc acgtacagat
    25741 tgtcggatgc agcgtaaacg ctttatccga cctggattcg cgtaggccgg acaagacgcg
    25801 cagcgtcgcc tccggcatgg gtacggatct actgcttcgc ctcatcaaaa cactggctga
    25861 tgatttccag accctgacgg atctgagcgt cttcaatggt gagcggtaca aggatgcgca
    25921 gcacgttgta atacgggccg caggagagaa gaatcaggcc tttatcgcgg gcacgcgcca
    25981 cgatctcggc ggtgagtttg gcgtccggtt tgctgtgatc gccatcttca aacagctcga
    26041 tggcaatcat cgctcccagc ccgcgtacat cgccgatctc cgggtgtttt tcggctatcg
    26101 ccagcaatcc gtctttcagc ttatgcccca ggtcgttggc tttttgcagc agattttcct
    26161 gctcgaatac ctttaacact tccagcgctg ccacgcaggc aatcgggtta ccggcatagg
    26221 tgccgcccag accgcctgga gcgacggcat ccatcacttc cgcgcgcccg gtgacgcccg
    26281 ccagcgggaa gccgcccgcg atcgatttcg caaaggtggt aagatccggc gcaacgccca
    26341 tctgctccat cgcaaacagc gtgccggtac gccccgcgcc gctctgcact tcatcggcaa
    26401 tcagcatgat cccgtgctcg tcacacagcg cgcgtaaacg ctgcataaag gctggcgtcg
    26461 cggcgtagaa accgccttcg ccctgaaccg gctcaatcac gatggcggcg atatcttccg
    26521 gcgcagcgtc atttttaaaa attcggtgaa tgctggcgat agcgtcatct tcactgatgc
    26581 cgtgcagcgg gcaaggataa agcgcgcgat aaacgtgccc aggcatcagt cccatgcccg
    26641 cagagtacgg attcaccttg ccggtcagcg ccagcgtgta atgcgtgcgc ccgtgatacg
    26701 cgccgctaaa ggcgatggtc ccgctgcgtt tggtggcggc gcgggcgatt ttcaccgcgt
    26761 tctccaccgc ttcagaaccg gtcgttacca gcagcgtttt tttggcgaaa tctcccggca
    26821 ccttctgatt cataatctcg cacagctcca gatacggttc gtaagccagc acctggaagc
    26881 aggtgtgcga cagctttttc aactgcgctt ccaccgccgc aaccactttc ggatgcaggt
    26941 gcccggtatt gagcaccgca atgccgcccg cgaaatcaat atactcacgg ccttcaacgt
    27001 cccacacccg gcagttttcc gcgcggtcag cgaaaatggg gtgaatttgc ccaacgccac
    27061 ggggaatcgc ctgactgcgg cgctgcatta actctttatt gctgctcatt cgcgttctcc
    27121 agttaaagac cgatgcacat atatttgatt tctaagtaat cttcgatgcc atacttcgaa
    27181 ccttcacgac ccagacccga ggctttaatg ccaccgaacg gggccacttc attggaaata
    27241 atgccagtat taatgccgac gatgccgtac tccagcgcct cgcccacgcg gaagacgcgg
    27301 cttaaatcac gggcgtagaa ataggcggcc aggccaaact cggtgtcatt ggcctgcgca
    27361 atcacatcgg cttcatcttt aaagcggaat agcggggcaa gggggccgaa cgtctcttct
    27421 ttcgacactt tggcgttggc cggaacgtcc accagaatgg tcggctggaa gaagttgccg
    27481 ccgcgttcat ccgctttacc gccgcaaacc acacgcgcac ctttctccag cgcatcggca
    27541 atatgctctt ccacttttgc taccgctttt tcatcaatca gcggcccgat ggtgacgcca
    27601 ttctccagcc cgtcgccgat gtgcagtttg ctcaccgcct gctgtaattt ttcggcaaag
    27661 cggtcataca cgccgtcctg cacgtataaa cggttggcgc agacgcaggt ttgcccggcg
    27721 ttgcggaatt tcgaggccag tgcgccttcc acggctttgt cgaggtcggc atcgtcaaag
    27781 acgataaacg gcgcgttgcc gcccagttcc agcgacactt tcttgatgtc tttcgcgcac
    27841 tgttccatta attggcggcc aatttcggtc gagccggtaa acgacagctt gcgcaccagc
    27901 ggattgctgg tcagttcgtt acccaccgcg cccgccgaac cggtgaccac gttaaatacg
    27961 ccagccggaa cgcctgcgcg aatcgccagc tccgccagcg ccagcgcaga gaacggcgtc
    28021 tgactggcgg gcttcagcac catggtgcag cctgccgcca gcgccggacc ggctttgcgg
    28081 gtaatcatcg ccgccgggaa gttccacggc gtgatagccg cggtgacgcc aatcggctgc
    28141 ttgataacaa tcaggcgttt atcggcctga tgaccaggaa tggtgtcgcc ataaatgcgt
    28201 ttgccttctt cggcaaacca ctcaataaag gaggcggcgt agctaatttc gcctttcgct
    28261 tcggccagcg gtttgccctg ttctagggtc atcaggcgcg ctaaatcgtc ctgatgctcc
    28321 atcatcaaat tgaaccagtt gcgcagaatg gtggcgcgtt ctttggcggt gagcgcacgc
    28381 caggcgggca gagcgcggtt ggcggcgtcg atagcggcgc gggtttcatc agcgcccatt
    28441 ttgggtacgc tacccagctt gtcgccgttc gccggattgg tgacgtcgat gacctcgcca
    28501 ttgttggcgt ccagccattc cccgttaatc aacgcctgct ggcggaataa gttactgtcg
    28561 ttaggtttca ttgttacgtc ctgttttcaa aggttattga ttaaatgcgg cgtgtaaggc
    28621 atccacacta cgtgccgctc gcagcgtgcg tccggggtta ctctggcttg ccaacagcgt
    28681 ttgtaccttg ctgacaatat gcgcaccaat aggaattgct gatgtcgctg ccggggaggg
    28741 cgcattgcag gtgtggatcg tgcgcggggt ggtgacaaac agaaaatcgt caatcagctt
    28801 gccgtccggc gataccgcct gcgcccgcac accagcgggc cagggctgga gatcgcttaa
    28861 cgaaagccgg ggacaatact tttgcaccag ccgcagatag ccgcttttgc acagcgagtt
    28921 tttcatctcg cccagtcctg agcgtagatg gttttgcagc acccggcgaa tccccgacga
    28981 gcccaaaatc tccagcgtgt cgctaaatga gaagtcgcgc ttacgatagc cttcgcgttt
    29041 gaaagccagc accgcgtttg gcccgacggt cacgctgcca tcgatcatgc gggtgagatg
    29101 cacgcccaaa aatggcatcg cggggtcggg aatggggtaa atcaggtggt taacaatctg
    29161 gttatgctcc ggcgcaaggc ggaaatactc gccacggaac gggcagataa taaagcccgg
    29221 ttcgaggccg agcattttca ccagccggtc agccatcagc ccggaacagc taatcagcgt
    29281 cgatgcttca tattcaccgc cctgacgggt acgtatcacc acgccgtttt tatgctcact
    29341 gagggcgctg acttcggcgt tataaataat ctcgccgcct ctggcctgga agatttttgc
    29401 catcgccgcc gtcacttcgc ggtagctgac aatgccgctg gacggcacaa aaatgccgcc
    29461 gagcccggtg atattcggtt cgcgctcgcg cagttcctcg gcgtttaacc actcgcgctc
    29521 gataccgttc gctgccgtgc gttcccacaa cgcgcgcatc cgttccattt cgagttcaga
    29581 ggtggcgacc agcatcttgc cgcagttgtc atagcgaatg ccgttttgat cgcaaaaggc
    29641 tttggtggcg cggtttcccg ccaggcaaaa ctgtgccttc aggctaccgg gcgtgtaata
    29701 gaccccggca tggatcacgc cactgttgtg gcccgtctgg tgacaggccg ggccggactc
    29761 tttttcctgc aacgcaatgc gggcatccgg ataaacatca atcagttgca tggcggtcga
    29821 catgccgatg atgccgccgc caataatcac aaaatcatac atccgctcaa ttcctttgcg
    29881 cttactgatg cgtctggtag tggttagtgg cgtaagcgaa atagccacgc tgacgcatca
    29941 gttcacggcg cagatccggg tgcggagtaa agcggtcgcg accgtgcagc cagaatagat
    30001 tgttaatcaa caggaatttg ccaacgggaa cgggcacaga aagaatgcct ttgctggttt
    30061 caatggcatc ggaaagctca ctcagccaca cgccttcttc gaagtctttt ggctggacga
    30121 actggtcgat ataacgcatc accgggcgac cctgttgatc aacgtcgaac actggatgga
    30181 aaacatcttt gctgacgttt ttgctcggag gcgcggcaaa gcgcatcggg cgacgcgcca
    30241 gcgggtggcg gaaatagtgg tccagatgtt cccagtcatc gagatgcagc agcaacgaat
    30301 ttccaccctg catgttctgc tcgtcgattt tcatcatcag cacatagtcg gtgatctctt
    30361 cgacgtaggt gccgtcgttg tgcagctcca ttacgcggtg cggctgacgc agatagctgt
    30421 ctgagttatc aacatttttc accacgaatc gcgcgtaata ctgaccgctc atcgcatcga
    30481 aatttgagcg accaatcaga tgcgctaccg ccgttgccag cttcaccatc tcatccgctt
    30541 gcttcacatc atcgacaccc accgcattga tcaacagcgc accttcagca cggtttagca
    30601 gggtcttcaa cagcaatggt tgcagctggt tcgcacacag atcgtcaaga attttgccta
    30661 cccgaaaacg cagaaaagat ttgtactcca gcgcctgcac gggccattcg gcaacctgtt
    30721 cgagaaactg tttggtcgtc tgttcggtga aggtgagttc cagcagacgc ggagattgcg
    30781 ccgacgggat gagagtgaat ccgctatagt cctggcctga atcgacagcg ttattttgta
    30841 cggcggtcag tgcattcatc agaagcgatc ctcttatgag atgtagggtg acatggcgat
    30901 gctcatttcg tagccataat ctaaaaatat ctacatttct gaaaaatgcg cataaaagtt
    30961 acatattgtt ttcttattgc gatcaaaaac aacaaattgg taacatttga cgtaggttat
    31021 taaggcaaag tgagacagga aatgaataac taaagcaaca ggttaggggt ggattaccat
    31081 cagagaacag attatctggt cgataaaaaa cggtaaatgg cttcactgca aaatgaccgt
    31141 tagtaaacta aacatattat ctgccccgtt ttagcggggc agatataaca ttaaattaca
    31201 aatagagata gttcggcggt tctccagttt tccctaaaag aagagcgcta cgcccaggaa
    31261 tattgatatt tagagttccg cctctaagca taaatgcttt accagggggg ataatttttc
    31321 gggtattatt aataataatg ttaacgctat tactccccat gtttttatat attccaggtt
    31381 gcaatttgtt aggccaggta ccttcaactt cttcgtttcc tttatttatt ataactagtc
    31441 catcatgagg agagtgatta atgttcacag aatccccacg actaaaggcc agaacgtttt
    31501 tagttgcaaa tacaccctca aatgcgctcc atattcctgc tttatctata tataatgcgt
    31561 ggaattcatt gtagagctgc tgataggtag agcaaccttt attaagccac gcctcaccgc
    31621 cgacacgcgt cgacccatca taatttttaa tttcagtcgt tgattttaat acttggccat
    31681 cccagtaaac taaaggcctg ctgaacggga gagcggccat atatgccatt gctaattgtt
    31741 catcttgttg atggttaaat agaaagtcat tgaaaagatt gccattgtta ggaaaatcgt
    31801 ggttaatgtt aatcaatatc cgtctttttg acgggatgct tgagattgtt gaacccggtt
    31861 ttgatgcaag ggtttggaga tcgccaccaa atgaaaaggc ttttatcgtc ttcatgagca
    31921 atgaaaaatc aatggcgctt aaatcatctc tttcataaag cagcgatgac atttgttctt
    31981 catccaactg acaagttacc aggtattcca tgtaattcat aacggctttt tcatgatata
    32041 ttggcttaaa taagttagta ttgtgtaatc gctcattata aattgtaagc ggtggagtta
    32101 ttgatctttc tatttgttca tgttttgagt gttttgctgc atcgtaacgt aaacctcgta
    32161 cgccatattt tcgcatttca cgaacgtatt gattttgcaa cataataacc tggtcacttt
    32221 tgggggttgt tttaggcatt ccatttaaac gcttttcttt taatgcatgg aggttttccc
    32281 attcatcctt agagatgtta tctttacttg tttcaaagtc ttttgcagtg agatgtatat
    32341 cttctggatg gatttgttta cgttcttttg ttactggatc tatagtaaat ggaatcaaaa
    32401 cctgaccttg ccaatagcca gggataccac tttctatatc tttactgatc ttcgctcgta
    32461 tatcagcaga gggataatct aggtcatttt taccactacc cccaattccc ataaagttaa
    32521 aggtataatc aggaatgata tcgataccaa attttttagc atctttaacc aatgcgataa
    32581 aatcctgcaa atctcccatt ggattatgga tggtacaaag atcttcgggt tgataagcgt
    32641 gaaaccagta acctttttct tcttcgggag tttctggtcc gtcggcgcgg aaagttgtca
    32701 ctggaggaat ttgtatatac ccataatggg aacgtgcaaa ctcagacata ttttccctga
    32761 tttgctggta ggtggcatta aaagcatgaa gaatcgcttc gccaggtttt ttgggggtga
    32821 tgatttcaca ttgtgatttc tgaacagcag taagagattt acgctcaagt gtttgccgta
    32881 gatgttctgg attgattagt attttaaatg tttcttgctg tccggaaggt gacgtcacgt
    32941 taatattata tacatcggct gctagctcat agtcaggaat gttaatagca tatgcaacat
    33001 acttatcttt tggggatggt ttgtcagata taatgtgagt gaaatgttca gctggaccat
    33061 actgatgaac gcctctgtta tcacctcttt caaccattgt atctcgttga taaacccatg
    33121 ttttcccttc tcctatcctg tctggcgctg taatgctacg ctgtgcagct ttattcagct
    33181 ctcggtaagt tgtatctgga gaaagggaaa ttttataatc gttttttaac agatcattaa
    33241 tgactttttc tgcgtgaaaa gttgatataa tttctctgtc ttttaaatcc tgaggataaa
    33301 ctccacaagc attaataata tttgttatac agtttagata ttctaatgta tttaatttgg
    33361 aatttagatc cccgtcagta atgttccatg ataacaatgt gggg
//
LOCUS       DAZGTJ010000052        33390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000052 DAZGTJ010000000
VERSION     DAZGTJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.052"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(475..2163)
                     /gene="betA"
                     /locus_tag="ACHK8E_002982"
     CDS             complement(475..2163)
                     /gene="betA"
                     /locus_tag="ACHK8E_002982"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP0345219.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKESVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
     gene            complement(2177..3649)
                     /gene="betB"
                     /locus_tag="ACHK8E_002983"
     CDS             complement(2177..3649)
                     /gene="betB"
                     /locus_tag="ACHK8E_002983"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP0345220.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3663..4250)
                     /gene="betI"
                     /locus_tag="ACHK8E_002984"
     CDS             complement(3663..4250)
                     /gene="betI"
                     /locus_tag="ACHK8E_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP0345221.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            4379..6412
                     /gene="betT"
                     /locus_tag="ACHK8E_002985"
     CDS             4379..6412
                     /gene="betT"
                     /locus_tag="ACHK8E_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP0345222.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            7289..8377
                     /gene="pdeL"
                     /locus_tag="ACHK8E_002986"
     CDS             7289..8377
                     /gene="pdeL"
                     /locus_tag="ACHK8E_002986"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFP0345223.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8419..9351)
                     /gene="yahB"
                     /locus_tag="ACHK8E_002987"
     CDS             complement(8419..9351)
                     /gene="yahB"
                     /locus_tag="ACHK8E_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0345224.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(9443..9940)
                     /gene="yahC"
                     /locus_tag="ACHK8E_002988"
     CDS             complement(9443..9940)
                     /gene="yahC"
                     /locus_tag="ACHK8E_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0345225.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10198..10803
                     /gene="yahD"
                     /locus_tag="ACHK8E_002989"
     CDS             10198..10803
                     /gene="yahD"
                     /locus_tag="ACHK8E_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0345226.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            10843..11706
                     /gene="yahE"
                     /locus_tag="ACHK8E_002990"
     CDS             10843..11706
                     /gene="yahE"
                     /locus_tag="ACHK8E_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0345227.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11696..13243
                     /gene="fdrA"
                     /locus_tag="ACHK8E_002991"
     CDS             11696..13243
                     /gene="fdrA"
                     /locus_tag="ACHK8E_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0345228.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13243..14661
                     /gene="yahG"
                     /locus_tag="ACHK8E_002992"
     CDS             13243..14661
                     /gene="yahG"
                     /locus_tag="ACHK8E_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0345229.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            14687..14941
                     /locus_tag="ACHK8E_002993"
                     /pseudo
     CDS             14687..14941
                     /locus_tag="ACHK8E_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14991..15941
                     /gene="yahI"
                     /locus_tag="ACHK8E_002994"
     CDS             14991..15941
                     /gene="yahI"
                     /locus_tag="ACHK8E_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP0345230.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            15951..17333
                     /gene="yahJ"
                     /locus_tag="ACHK8E_002995"
     CDS             15951..17333
                     /gene="yahJ"
                     /locus_tag="ACHK8E_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0345231.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            17629..18039
                     /locus_tag="ACHK8E_002996"
     CDS             17629..18039
                     /locus_tag="ACHK8E_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0345232.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            18293..19276
                     /locus_tag="ACHK8E_002997"
     CDS             18293..19276
                     /locus_tag="ACHK8E_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0345233.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            19325..20809
                     /locus_tag="ACHK8E_002998"
     CDS             19325..20809
                     /locus_tag="ACHK8E_002998"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0345234.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            20802..21773
                     /locus_tag="ACHK8E_002999"
     CDS             20802..21773
                     /locus_tag="ACHK8E_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0345235.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSILGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            21770..22726
                     /locus_tag="ACHK8E_003000"
     CDS             21770..22726
                     /locus_tag="ACHK8E_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0345236.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            22813..23862
                     /gene="yahK"
                     /locus_tag="ACHK8E_003001"
     CDS             22813..23862
                     /gene="yahK"
                     /locus_tag="ACHK8E_003001"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP0345237.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            24484..24729
                     /gene="yahM"
                     /locus_tag="ACHK8E_003002"
     CDS             24484..24729
                     /gene="yahM"
                     /locus_tag="ACHK8E_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP0345238.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(24746..25417)
                     /gene="yahN"
                     /locus_tag="ACHK8E_003003"
     CDS             complement(24746..25417)
                     /gene="yahN"
                     /locus_tag="ACHK8E_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP0345239.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            25564..25839
                     /gene="yahO"
                     /locus_tag="ACHK8E_003004"
     CDS             25564..25839
                     /gene="yahO"
                     /locus_tag="ACHK8E_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP0345240.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(25940..27526)
                     /gene="prpR"
                     /locus_tag="ACHK8E_003005"
     CDS             complement(25940..27526)
                     /gene="prpR"
                     /locus_tag="ACHK8E_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP0345241.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            27765..28655
                     /gene="prpB"
                     /locus_tag="ACHK8E_003006"
     CDS             27765..28655
                     /gene="prpB"
                     /locus_tag="ACHK8E_003006"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP0345242.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            28815..29984
                     /gene="prpC"
                     /locus_tag="ACHK8E_003007"
     CDS             28815..29984
                     /gene="prpC"
                     /locus_tag="ACHK8E_003007"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP0345243.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            30018..31469
                     /gene="prpD"
                     /locus_tag="ACHK8E_003008"
     CDS             30018..31469
                     /gene="prpD"
                     /locus_tag="ACHK8E_003008"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP0345244.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            31509..>33390
                     /gene="prpE"
                     /locus_tag="ACHK8E_003009"
     CDS             31509..>33390
                     /gene="prpE"
                     /locus_tag="ACHK8E_003009"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP0345245.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AME"
BASE COUNT         7971 a         8328 c         9036 g         8055 t
ORIGIN      
        1 tgatgactga ttaaagaagg gtaaaaataa atcgctgcta ataaattata cgaaccgtgt
       61 ttaaagctcc acgtagtttt tggggctgac ttatttttat actcgccaca agtccccttt
      121 tcgtgcatga taaccgaata aattgacttt tttagcgtag acctgatatt actccacgct
      181 ttgggataaa tacgcccggc ttacgcagtt gatcgctgac cactgctaac cgggagtggg
      241 aaagaacaag taaccctagc caggagcact ggttgcgtga ttcttaagct gagtggcatt
      301 acacttgtgg tttatcagcg tatccatagc accatcatcc gccagattga gcgaatcggc
      361 ttaaagtttg atggcgggac tgcggatatg aagcatgaga gttacctcag tgttttatat
      421 aaggattcgg tcctcatagt agaacggtaa ctctcaatct gataggttcc tgagttagtt
      481 cacatcacga ctcatttttt tcgctctcac cggcatccca tttgccacaa aatatcttgc
      541 cgtgctcctc ggcaacgctt cttgcccgcg aatcatatcc gccattttct cgccaatcat
      601 aatcgtcgta gcgttcaaat tgccggtgat aatttgcggc ataattgacg catccaccac
      661 gcgcaggcct tccagcccgt gtacgcggcc ttcggcgtca accactgcca tctcgtcata
      721 acccattttg caggtaccgc acggatgaaa cgcggtttcg gcgtggttac gcacgaactc
      781 atcgagctgc tcatccgtct gacattccgc gccggggctg atttcgcggc cacgatactg
      841 atccagcgcc ggttgatgca tgatctcccg ggtgatgcga attgcgtcgc ggaactcctg
      901 ccagtcctgc tcgtgcgaca tgtagttaaa cagaatcgct ggatgctggt gcgggtcgcg
      961 ggatttaatc cgcacatgcc cacggcttgg cgagcgcatc gatccgacgt ggcactggaa
     1021 gccgtgctct ttcactgcgt tcgagccgtt atagttaatc gccactggca ggaaatggta
     1081 ctgaatattc ggccacgcaa attcctcacg gctgcgaata aatccgcctg cttcaaagtg
     1141 gttgctggca ccaacgccgg tgccgccaaa cagccactcc gcaccgattt tcggctgatt
     1201 ccaccactgc agggcagggt agagggaaac cgattctttg cactcatatt gcagatacat
     1261 ctccaggtga tcctgaagat tttcgccgac gccgggtaaa tcatgcacca gcggaatatc
     1321 aaactccgcc agcagttcag cgttgccgac gccggagcgt tgcaggatct gcggtgaagc
     1381 aatcgcgcct gcacataaca gcacttcttt attcgccgcc gcgcgggtcg gaatggtgct
     1441 gtcgccttcc agccactcga cgcccaccgc gcgtttgccg tcaaaaatga tgtgatcggt
     1501 catggcgtga gtacgaatgg ttaggttagg gcgcgatttg gcctgatcga gatagccacg
     1561 cgcggtgctg gcgcgacggc cctgcggcgt gacggtgcga tccatcgggc caaaaccttc
     1621 ctgctgatag ccgttgagat cgtccgtgcg cgggtagccc gcctgcacgc ccgcttcaat
     1681 catcgcttca aacagcggat tgacgccggg tttggaggtg gtgacgctca ccgggccatc
     1741 accgccgtgg tagtcgttct cgcccacatc gcgagtctcg gccttgcgat agtagggcag
     1801 acagtcgagg tagctccagt tctccagacc gggttctttc gcccagttat cgagatccag
     1861 cgcgttacca cggatgtaac acatgccgtt gatcagtgat gacccgccaa gccccttacc
     1921 gcgtccgcac tccatgcgac ggttgttcat aaacggttcc ggttccgtct cataggccca
     1981 gttatagcgt ttaccctgta acgggaacgc cagcgcggcg ggcatctggg tgcggaagtc
     2041 aaagcgatag tccgggccgc ctgcttcaag cagcagcacg gttgtgttcg gatcttcagt
     2101 cagacgggta gcgagaacgt tgccagctga gccggcacca ataatgatgt agtcaaattg
     2161 caaataaacc tcctggttag aatatggact ggaatttagc catctcaacc tggatggact
     2221 tcacctgggt gtaactctgg agcgtcatca cgccgttctc gcgaccaatg ccggagtgtt
     2281 tgtagccgcc aacgggcatc tctgcagggg attcgcccca ggtgttgatc cagcaaatac
     2341 ccgcttccag ctgatgaatg acgcgatgcg cgcggttcag gtccgccgtc acgatgcccg
     2401 ccgccaggcc gtagtcggta tcattggcgc ggcgaatcac ttcgtcctca gattcataag
     2461 tgagaagcga catcaccgga ccgaagatct cttcgcgcac gatggtcatt tcgtcgcggc
     2521 aatcggtgaa cacggtcggt gcaacccatg cgccgttatc gaagccatcg cctttcagta
     2581 catcgccgcc gcacagtacg cgcgcgcctt cctctttgcc tttggcgatg taacgcagca
     2641 cgttatcgcg atgcgggaag ctgaccagcg ggccaaagtt agtttgcgga tcgaaaacgt
     2701 cgcccgcgcg aatgcgctca acgcgcgcca gaatcttttg ttcaaatgcg gctttgcatt
     2761 tcgccggaat aaacacgcgg gtgccgttgg tacacacctg accggagctg aagaagttcg
     2821 ccatcatggc gatatcagcg gcgagatcga gatccgcatc gtcgaaaacg atcagcggtg
     2881 atttaccgcc cagctccatg gtcacttctt tcagggagga agccgccgag ttagccatca
     2941 cttttttgcc gctggcgaca ccgccggtaa atgacacttt ggcaatgccc ggatgctcgg
     3001 tcagatactg cccggtttcc gcacctacgc ccggcagcac gttaaatacg ccgtccggca
     3061 ggcccgcttc gctgtaaatt tcggccagct ttaacgcggt aagcggggtg acttcgctcg
     3121 gtttgaaaat catcgcgttg cctgccgcca gcgccggggc ggatttccac aaggcaatct
     3181 ggatcgggta gttccatgcg ccaatccctg ccactacgcc cagcggttcg cggcgggtat
     3241 aaacaaatga cgtctcacgc aatgggatct ggctgccttc cagcgacggg atcagcccgg
     3301 cgtagtactc cagcacgtcc gcaccggtaa cgatatcgac ggttgaggtt tccgaatacg
     3361 cttttccggt gtcgagggtt tccagttttg cgagttcgtc attgcgttca cgcagaatat
     3421 caacggcccg acgcagaata cgcgagcgct ccatagcggt catcgccgcc cagatttttt
     3481 gcccttgttg ggcactcttc acggcgcgat cgacatcctc acgcccggcg gcctgcacgg
     3541 tcgccagcac gttaccgttg gccgggttaa tggtctcgaa ggtgcgaccg ctggtggcgg
     3601 aggtataacc accatgtata taaagctgct gttctgccat tcgggacatc acgtttcctc
     3661 ggttaatcgg tgggtaaatg ctgagtaata aagtgacggg tcagcgagtg tgcgagaggt
     3721 ttatccagcg ctttgccgct cagtgccgcg cgcagccata atccgtcaat cagcgcggca
     3781 aggccgtagc ccgcttcctg tgcctgttgg cgcggtaact cgcggcgaaa ttcgctcacc
     3841 agattcgaca ataagcggcg gctactgacc tgctgcaagc gatagagcat cggctgatgc
     3901 atactgctgg cccagaaggc cagccaggct ttcatcgccg cactgctcac ctgggtttca
     3961 tcgaagtttc caccaacaat cgcctgtaat cgcagctctg cactgccctg cggaagtgca
     4021 tgtaatcgat tcaaaaccgc gtcacgcagc tgactggtga tatcgcgcat ggttgcttcc
     4081 agcagaccat ttttgtccct gaaatagtgg ctgatgatcc ccgtagaaac gcctgcacgg
     4141 cgggcgatct gcgcgatcgt tgcatcgtgc atgcccactt catttattgc ttccagcgtg
     4201 gcgtcgatca gttgtctgcg ccggatcgac tgcatcccca atttgggcat tttcgccact
     4261 ccattcatca gcgttgttta tctattaaag cggttattga ttggacgttc aatataaaat
     4321 gtgtcttaat tgttacgaat ttgattttaa atggtaacaa taacagtggg gatactggat
     4381 gacagacctt tcacacagca gggaaaagga caaaatcaat ccggtggtgt tttacacctc
     4441 cgccggactg attttgttgt tttccctgac aacgatcctg tttcgcgact tctcggccct
     4501 gtggattggc cgcacgctgg actgggtttc taaaaccttc ggttggtact atctgctggc
     4561 ggcaacgctc tatattgtct ttgtggtctg tatcgcttgt tcgcgttttg gttcggtgaa
     4621 gctcgggcca gaacaatcca aaccggaatt cagtctgctg agttgggcgg cgatgctgtt
     4681 tgctgccggg atcggtatcg acctgatgtt cttctccgta gccgaaccgg taacgcagta
     4741 tatgcagccg ccggaaggcg cgggacagac gattgaggcc gcgcgtcagg cgatggtctg
     4801 gacgctgttt cactacggct taaccggctg gtcgatgtat gcgctgatgg gcatggcgct
     4861 cggatacttt agttatcgtt ataatttgcc gctcaccatc cgctcggcgc tgtacccgat
     4921 cttcggtaaa cggattaacg ggccgattgg tcattcagtg gatattgcag cggtgatcgg
     4981 caccatcttc ggtattgcca ctacgctcgg tatcggtgtg gtgcagctta actatggctt
     5041 gagcgtactg tttgatattc ccgattcgat ggcggcgaaa gcggcactga tcgccttgtc
     5101 ggtgatcatc gccactatct cggtgacatc cggtgttgat aagggcattc gcgtgttatc
     5161 ggaactcaac gttgcgctgg cgctgggatt gatcctgttc gtgttgttta tgggcgacac
     5221 ctcgttcctg cttaatgcac tggtgctgaa tgttggcgac tatgtgaatc gctttatggg
     5281 catgacgctc aacagttttg ccttcgaccg tccggttgag tggatgaata actggacgct
     5341 cttcttctgg gcatggtggg tggcatggtc gccgtttgtc ggcttgttcc tggcgcgtat
     5401 ctcgcgtggg cgtaccattc gccagttcgt gctgggcacg ttgattattc cgtttacctt
     5461 cacgctgtta tggctctcgg tattcggcaa tagcgcgctg tatgaaatca tccacggcgg
     5521 cgcggcattt gccgaggaag cgatggtcca tccggagcgc ggcttctaca gcctgctggc
     5581 gcaatatccg gcgtttacct ttagcgcctc cgtcgccacc attactggcc tgctgtttta
     5641 tgtgacctcg gcggactccg gtgcgctggt gctggggaat ttcacctcgc agcttaaaga
     5701 tatcaacagc gacgcccccg gctggctgcg cgtcttctgg tcggtggcga ttggcctgct
     5761 gacgctcggc atgctgatga ccaacgggat atccgcgctg caaaacacca cggtaatcat
     5821 ggggctgccg ttcagctttg tgattttctt cgtgatggcg gggttgtata aatctctgaa
     5881 ggtagaagat taccgccgtg aaagtgcgaa ccgcgatacc gcgccgcgac cgctggggct
     5941 tcaggatcgc ctgagctgga aaaaacgtct ctcgcgcctg atgaattatc cgggcacgcg
     6001 ttacactaaa cagatgatgg agacggtctg ttacccggca atggaagaag tggcgcagga
     6061 gctgcggttg cgcggcgcat acgtggagct aaaaagcctg ccgccggaag agggacaaca
     6121 gttggggcat ctggatttgt tggtgcatat gggcgaagaa caaaactttg tctatcagat
     6181 ttggccgcag caatattcgg tgccgggctt tacctaccgc gcacgtagcg gtaaatcgac
     6241 ctactaccgg ctggaaacct tcctgttaga aggcagccag ggcaacgacc tgatggacta
     6301 cagcaaagag caggtgatca ccgatattct tgaccagtac gagcggcacc ttaactttat
     6361 tcatctccat cgtgaagcgc cgggccatag cgtgatgttc ccggacgcgt gattgttatt
     6421 gcataaaacc gcgccatgta tgcatatggc gcggtttctc ttacctcttg atacgttata
     6481 tctgtacagt taagccctta gtatagattg atgattgcca ggcaacagat aataaaaaag
     6541 aaaagaacta ttgaagccca aaacctacgt ttggactgtt gtgaatgttc aataagctta
     6601 gtcttaatta atgtaaatat tggtgctaat ttgaaatgac gctttattca tggggaacgc
     6661 gtagcgcaaa tcaacaaaaa tcaatgtaac catttgttgt ttaaggttta attgtctgtg
     6721 tctgatattt tattgattgt tttaaatatt gtttttattg tcattgttat aatgttggtc
     6781 atctattgct gaatggattc agtcttaaga ggcaggttgt taagggatag gcatagagta
     6841 atgacgtgta tgcataacca acatctttac tcattatgtc attgaatgtt gacgctatgt
     6901 gtttatgagg ttgaggtatt ttcagttgat ctggattgtt aaattcatat aatgcgcctt
     6961 tactcatgaa tgggcgtcaa tacgtagtgg gaaattataa atatcgaaat agtccaacta
     7021 cttctttatt accaaaaatg agtatctgaa ttttaatatt gcattcttgc gtgattatct
     7081 cctgagtttt gacttgtgat taccttttta aggtatttag cgtaactgtt tctgagcgag
     7141 catcagaggt aaagataatc ttcttgatag tgatgtggga tgttatacgt atggcatcgc
     7201 taatgtttat ggttacccct tatgtgtgct caggaatcca caggtaatca ctcatactga
     7261 acagcgataa aagataaaag gtgtgttcat gaattcatgt gattttcgtg tttttctgca
     7321 agagttcggt acaacggttc atttgtcatt gcctggtagc gtatccgaga aagaacgact
     7381 gctactcaag ctgctgatgc agggaatgtc tgtaacagaa atatcacagt acagaaatcg
     7441 tagtgcaaag accatttcac atcaaaagaa acagctcttt gagaaactgg ggattcagag
     7501 cgatattact ttctggcggg atattttctt tcagtacaat ccggagatca tatccgccac
     7561 gggtaataat agtcacaaat atattaatga taatcactat caccatatcg tcacgcctga
     7621 agccatcagt ctggcgttgg aaaaccatga atttaaaccg tggatccaac cggttttctg
     7681 cgcgcagact ggggtactga cgggctgtga ggtgcttgtc cgctgggaac atccacaaac
     7741 gggaattatc ccaccggatc agtttattcc tctggcggag tcatccggtc ttattgtcat
     7801 aatgacccgc cagttgatga aacagactgc ggatattctg atgccggtaa aacatttgct
     7861 gccggacaat ttccatattg gcatcaacgt ctcggcgggt tgttttttgg ctgcgggatt
     7921 tgaaaaagag tgtctgaacc tggttaagaa attaggtaac gataaaatca aactggttct
     7981 cgagctaacg gaacgtaacc ctattccggt aacgccagaa gccagagcga tatttgacag
     8041 ccttcatcag cacaacatta cctttgcgct ggatgacttt ggtacgggtt atgcgaccta
     8101 tcgttacttg caggcgttcc cggtcgattt tattaagatc gataagtcat ttgtgcaaat
     8161 ggcgagcgtg gacgaaatat ccggtcatat tgtggacaat attgtcgaac tggcgcgtaa
     8221 gcctggtctg agtatcgtgg cggaaggggt agaaacccag gagcaggcgg atttaatgat
     8281 cggcaaaggc gttcactttt tgcagggcta tttgtactct ccgccagtac cgggtaataa
     8341 atttatctct gaatgggtaa tgaaagcagg tggttgatgt aaaccgctat tcacagcgca
     8401 tcgggaggtt ggcagcgatt aattctcccg atgcagtaaa tcctgataga tccccgttaa
     8461 tatgccattt ggcgaaaatt gttttttaat ccactgcgtc acctgacccg tagcagaatg
     8521 ctgagttgcg agtaacatgc gtgaatcctg gcgcggatta tggatttgtc gggtaaccag
     8581 cagggattgc gccatcgcct cacggaccat gtaatccggc aaaaaaccaa ttccttcacc
     8641 taaaatctgg cactgacatt tggtgttgaa atctggcacc agaatcgact cctgcccatg
     8701 cagcaaccag cccacttttt tattaatcgt atgcgcggtg tcctccacca tgatattggg
     8761 gtaaagacgc agctggcttt cggcgatcgg ctccggcaca aaggctaatg ggtgatccgg
     8821 ggcgatagca aaagcccagc gaattgcgcc aatctccgtg taatcaatgc cgccgccgtc
     8881 cagcagcgta tcgggtgcgc cgatagcaat attcgcctga ttattaataa tcgcatccca
     8941 gacgccgtta tacacttcgg tcgtcacggt aatctggcag gttggaaact gctttttcag
     9001 tacctgtaat aagcgtgcgg tgtgtttggg cgtataaagc agttggttaa tacaaatgcg
     9061 cacccgtgct tctataccct gcgaaatagt atcgatgctg cgtttgatgg cgtgaaaatc
     9121 attcagcagg tcggtagctt tgcggaaaaa ataacgcccg gactccgtta actcaatgct
     9181 tcgggtactg cgagtgaaca gcaccacatc cagccctgtt tccattcgct tgatggtgta
     9241 gctaatggcg gaagtggtta aacccaactc ttctgcggct ttactgaaac tgccaaaacg
     9301 cgcagcggta gtaaatgcca acagattctc ttcggtaaaa attgagttca ttaaaccatt
     9361 cccgccatta tcgaatatga atttctccag ctatgactgt agaggtatcg gttaaagata
     9421 gataatcatt tttgaataac ttttaatacc cgtcgcgttt agtcgtcgct tcgcttaatt
     9481 tttgtccgcc atattctgaa attatcccca cgcagttacc cgccagaaaa cccagtaatg
     9541 tcaccgtcca gttcatcccg ctggcaaaaa atagcgtcat gccgagaaag cctccgggaa
     9601 taaaagacag taaaccgaaa cgtccttgcc agaccaccac cgccgagaag ggtacagtca
     9661 taatcacgct cgcccagaac ccggaaatcc cacttacaga ggccagccag ccggcagcca
     9721 gcgcggcgaa aaaggcccac accatcccga aataattaac cgccagactt ctgataaacc
     9781 ccgatttacc gccgcctgcg gcataaaaac tgcaaaaggc gacgaagcca atggttccta
     9841 ataattccca tccctgagag agcccgacat gtgaagacac taactgccaa agccctgcac
     9901 aaataccgac ggtgacaccc gttgccgtga gtccgttcat cttgttcccc tctggttaga
     9961 catggacgaa agctactctt acatgtgcag actatagaga aataaaacct gattgagtga
    10021 aagggtatcg ggtcaaagaa acaaatattg cactaccgca cactgcgaaa agattgttga
    10081 acatcattca acaaaaaggc gaaatacggc gaattaaatt taagcgggtt cttttatcag
    10141 attatcgttg ctattctcaa ttcatcgaaa acagccacaa aaaccagggg aatgtgaatg
    10201 actataaaaa atctacctgc cgactattta ttggctgcgc aacagggtga tatcgataaa
    10261 gtaaaaacct gccttgcgct gggtgtcgat attaatacct gcgatcgtca ggggaaaacg
    10321 gcaattacgc tggcaagttt atatcagcaa tatgcttgcg ttcaggcatt aattgatgcc
    10381 ggagcggata ttaataagca agatcatacc tgtttaaatc cttttttaat tagctgtctg
    10441 aacgatgatt taacgctact acgaattatt ttaccggcta aacccgatct taattgcgtc
    10501 acccgttttg gcggtgtcgg cctgacgcct gcctgtgaaa aaggccattt aaatattgta
    10561 aaagagcttt tggcgcatac ggagattaac gttaaccaga ccaaccatgt tggctggacg
    10621 ccgctactgg aagcgattgt gcttaatgat ggcgggatta aacagcaggc gattgtccag
    10681 ttattgctgg aacacggtgc cagcccgcac atgacggata aatatggcaa aacgccgctg
    10741 gaactggcgc gggaacgagg ctttgaagag attgcgcagt tactgattgc cgcaggtgca
    10801 taaaccggga ggcttgctat caacacacca gaaagacggt gtgtgtgggc gctaactgcg
    10861 gatgctgatt ttctggcgca gcgggggcaa ggacaggttg aacaggtctt tgccagagcg
    10921 gtaaatatcg cacttccggc ccgccagcag ttgctgacgc tgctttgtga agagtacgac
    10981 aatgcgccaa acagttgtcg gttggcactc actcactttg atggtctgtt ccggcatggt
    11041 gataaggttc agtttgacga tcaaggcatt acggttggtc aacatcttca tatagagatg
    11101 agtcgttgtc ggcgttggct gtccccaacc ttgcaaatgg ccgctgtgaa ttttcacctt
    11161 atcgcctggc aacagtggca tgacattatt catcagcacc tgggggaaaa tgaaaccctg
    11221 tttaattatt gcggcgataa tccgttttat caggcgttaa ataaagaatt acatattaaa
    11281 cgacgggcag ttattcaggc cgtaaacgat aaacaaaata tcgcctcagc agtcgccagt
    11341 atgatgggct tagggattgg ccttacgcca tcagccgacg attatttaac aggtctggcg
    11401 cttattttat ttatttccgg acatccggcg gaaaaataca aagaggaatt ttatctcggt
    11461 ctgcaatgcg gcagaaataa taccacgtta ttaagtgcca taacgctgga agccgcatta
    11521 caacaacgct gccgggaaaa tattcatcgt tttattcata acattattta tgacatccct
    11581 gggaacgcaa ctcaggcaat agaaaaaatt aaacatattg gctccagttc cggctgcgac
    11641 atgctgtatg gcatggccga tggttgtgcg ctgagccaaa cctacggagg gaattatgtc
    11701 agttaaaata gtcattaaac cgaataccta ttttgattct gtctcgctga tgtctatctc
    11761 cacgcgtgca aataaactcg acggcgtcga gcaggcattt gtggcgatgg cgaccgaaat
    11821 gaacaaaggc gtgctgagga atttaggact gctgacgccg gaactggagc aggcgaaaaa
    11881 cggtgacctg atgattgtca tcaatggtaa atcgggtgcg gacaacgagc agttgctggt
    11941 ggagattgaa gaactgttca acagcaaagc gcaaagtggc tcgcacgagg cgcgttacgc
    12001 cactattgcc agcgccaaaa agcatatccc ggaaagtaac ctggcggtga tttcggtcaa
    12061 cggtctgttt gccgctcgcg aagcgcgtca ggcgctgcaa aacgatctta acgtgatgct
    12121 gttttccgat aacgtctcag ttgaagatga actggcgctc aaacagttgg cccacgaaaa
    12181 agggctgctg atgatggggc cagactgcgg tacggcgatt atcaacggcg cggcgctctg
    12241 ctttggtaac gccgtgcgtc gcggcaacat cggcattgtt ggcgcatccg gcaccggtag
    12301 ccaggaactg agcgtccgca ttcatgaatt tggcggcggc gtttcgcaac tgatcggcac
    12361 cggcgggcgg gacctgagcg agaaaatcgg cggcctgatg atgctcgacg ctatcgggat
    12421 gctggaaaac gatccgcaaa ctgaaatcat tgtgcttatc tccaaaccgc cagcgcctgc
    12481 ggtggcccgc aaagtgctgg aacgcgcacg cgcctgccgt aagccagtgg tagtctgctt
    12541 cctcggtcgt ggcgaaacgc cagtggatga gcaggggcta cagtttgctc gcggcaccaa
    12601 agaagcggca ctaaaagcgg tgatgctctc cggtatgaaa caagaacatc tcgacttgca
    12661 taccctgaac cagccgttga ttgcggatgt gcgtgcgcgt atgcaaccgc agcagaaata
    12721 cattcgtggc ctgttctgcg gcggcacgct gtgtgacgaa accatgtttg cggtgatgga
    12781 aaaacatggc gatgtctaca gcaacattca gcccgatcct gaattccgcc tgcaagatat
    12841 caaccgcagc gtcaaacata ccttcctcga ctttggcgat gacgatttta ccaacggcaa
    12901 gccgcatcca atgattgacc cgaccaaccg catcagccgg ttgatcgaag aagcgcgcga
    12961 tccagaagtg gcggtgatcg tgatggattt tgtgctcggg tttggatcgc atgaagatcc
    13021 ggtcggttcc accatcgagg cgatcaaaga ggcgaaagcg atcgccgctg ccgaagggcg
    13081 cgagttgatc attctcgcct atgtgctggg taccgatctc gatacgccat cgttagaaca
    13141 acaaagccag atgttgcttg atgccggagt gattctggcc agcagcagca ccaataccgg
    13201 attgctggcg cgtgaattta tctgcaaagg ggaggaagcc tgatgagccc gtcactgttt
    13261 agccaaccgt tgaacgttat taacgtcggt atcgccatgt ttagcgatga tctgaaaaag
    13321 cagcatgtag aagtgaccca actcgactgg acgccgccgg ggcagggcaa tatgcaggtg
    13381 gtgcaggcgc tggacaacat tgccgattcc ccgctggcgg acaaaatcgc cgccgctaac
    13441 cagcaggcgc tggagcgcat tatccagtcg catccggtgc tgattggttt tgatcaggcg
    13501 attaacgtgg tgccgggtat gacgccgaaa accattcttc acgccgggcc gccgatcacc
    13561 tgggaaaaaa tgtgtggcgc gatgaaaggc gcggtcaccg gagcgctggt gttcgaaggg
    13621 ctggcgaaag atctcgatga ggcggctgaa ctggctgcct ccggggagat caccttctcg
    13681 ccgtgccacg agcacgactg cgtgggttcg atggcgggtg ttacctcggc ctcgatgttt
    13741 atgcacatcg taaaaaacaa aacctacggc aacatcgctt acaccaatat gagcgagcag
    13801 atggcgaaga ttttgcgtat gggcgctaac gaccagagcg tgattgaccg cctcaactgg
    13861 atgcgcgatg tgcaggggcc catactgcgc gacgcgatga aaattatcgg cgaaatcgat
    13921 ctgcgcttaa tgctggcgca agcgctgcat atgggcgatg agtgccataa ccgcaataac
    13981 gccgggacga cgctgctgat tcaggcgctg acgccaggga ttattcaggc gggctattcc
    14041 gtcgaacaac agcgggaagt gtttgagttt gtcgccagca gcgactactt ctccggcccg
    14101 acgtggatgg cgatgtgtaa agcggcgatg gatgcggcgc acggcattga atacagcact
    14161 gtggtcacca ccatggcgcg taacggcgtc gagttcggcc tgcgggtcag cggcctgccg
    14221 gggcaatggt ttaccggccc ggcgcagcag gtgatcggcc cgatgtttgc cggttataag
    14281 ccggaagatt cggggctgga tatcggcgac agcgccatca ctgaaaccta cggcattggc
    14341 ggatttgcca tggcgaccgc gcctgcaatc gttgcgctgg tgggcggcac ggtggaagag
    14401 gctatcgatt tctcccgcca gatgcgtgaa atcactctcg gtgaaaaccc caacgtcacc
    14461 attccgctgc tcggttttat gggggtgccg acggcaatcg acatcacccg cgtgggcagt
    14521 agcggcattc tgccggtgat caacaccgcc atcgcccata aagatgcggg cgtcggcatg
    14581 attggcgcgg gcattgtgca tccaccattc gcctgcttcg agaaagccat tcttggctgg
    14641 tgcgaacgtt acggcgtctg agttacatgt ttatgccgga tgcgacatga atgtcttatc
    14701 cggcctacga atggcgcgga aatctgtagg cctgataagc gtggcgcttc aggcattggc
    14761 caccacagcc ggatgcgacg tgaacgcctt atccggccta cgaatggcac ggaaatctgt
    14821 aggcctgata agcgtagcgc atcaggcatt ggtcaccaca gccggatgcg gcgtgaacgc
    14881 cttatccggc ctacgagtgg cgcaagaatc tgtaggcctg ataagcgtag cgcatcaggc
    14941 agtctggcgt tggtcataac cccatcaccc tctgtagcgg acataaaaat atgaaagaac
    15001 ttgtggtcgt tgccattggt ggcaacagca ttatcaaaga taacgccagc cagtcgattg
    15061 agcatcaggc ggaggcggtg aaagccgtcg ccgatacggt gctggaaatg ctggcttccg
    15121 attacgacat tgtgctgacc cacggtaacg ggccgcaggt cgggctggat ttacgccgcg
    15181 cggagattgc tcacgagcgc gaagggctgc ccttaacgcc gctggcaaac tgtgtggcgg
    15241 atacgcaagg tggcattggc tatctgatcc agcaggcgct gaacaaccgg ctggcccgcc
    15301 acggcgagaa aaaagccgtc accgtggtga ctcaggtgga agtggataaa aacgatccgg
    15361 gatttgccca tcccaccaag cctatcggcg tattcttcag tgaaagccag cgtgacgaat
    15421 tacaaaaggc aaaccctgac tggcgttttg ttgaagatgc cggacggggc tatcgccgcg
    15481 tggtcgcctc gccggaaccg caacgtattg tagaagcacc tgccattaag gccctgatcc
    15541 aacaaggctt tgtggtgatt ggcgcgggcg gcggcggaat tcctgtggtg tgcagtgaag
    15601 cgggagatta ccaaagcgtg gacgcggtta tcgacaaaga tctctctacc gcgctattgg
    15661 cccgtgaaat tcacgccgac attctggtaa ttaccaccgg cgtcgaaaaa gtgtgtattc
    15721 actttggcaa accgcagcag caggcgctgg atcgggtgga tattgccacc atgacccgct
    15781 atatgcagga agggcatttc ccacccggca gcatgttgcc aaaaatcatc gccagcctga
    15841 cgttcctgga gcagggcggt aaagaagtga ttatcaccac gccggaatgc ctgcctgcgg
    15901 cgctgcgtgg tgaaacgggc acccatatta ttaaaacgta aggacgtaag atgaaagaaa
    15961 acaatagccg ccgtgagttt ctgagccaga gcggtaagat ggtcaccgcc gccgcgctgt
    16021 ttggtacctc tgtgccgctc gcccatgcgg cagtctctgg cacaatccat tgcgacgcga
    16081 ataacaccat gaaaatcact gacccgcact actatctcga caacgtgttg ctggaaaccg
    16141 gttttgacta cgaaaatggc gtggcggtgc agacccgcac ggcgcgccag actgtggaaa
    16201 ttcagaacgg caaaattgtt gccctgcgcg agaacaatca gcatccggac gccacgctgc
    16261 cgcactatga cgctggcggt aagctgatgc tgcccaccac ccgcgacatg catattcatc
    16321 tcgacaaaac cttctatggc gggccgtggc gctcgctcaa tcgtccggca ggcaccacca
    16381 tccaggacat gatcaaactc gagcagaaaa tgctgccgga gttgcaaccg tacactcagg
    16441 aaagggcgga aaaactgatt gatttattgc agtcgaaagg cactaccatt gcccgcagcc
    16501 actgcaatat cgaaccggtt tccggcctga aaaatctgca aaatttgcag gcggttctgg
    16561 cgcgacgtca ggcgggcttc gagtgtgaaa ttgtcgcctt cccgcagcac ggtttgctgc
    16621 tgtcgaaatc agaaccctta atgcgcgaag cgatgcaggc gggggcgcat tacgtcggcg
    16681 ggctggaccc gaccagtgtt gatggcgcga tggaaaaatc cctcgacacc atgttccaga
    16741 ttgcgctgga ctacgacaaa ggcgtcgata ttcacctgca cgaaaccact ccggcaggcg
    16801 tggcagccat caattatatg gttgaaacgg tagagaaaac gccacagctg aagggcaagc
    16861 tgaccatcag ccacgccttc gcgttggcaa cgctcaacga acaacaggta gatgaactgg
    16921 cgaaccggat ggcggcgcag caaatttcta tcgcctcgac ggtgcagatc ggcacgctac
    16981 atatgccgct taaacaactg cacgacaaag gcgtaaaagt catgaccgga actgacagcg
    17041 ttattgacca ctggtcgcct tacggcctgg gcgacatgct ggaaaaagcc aatctctacg
    17101 cgcagctcta tattcgtcct aacgaacaga acctctcccg ttcgctgttt ttagctactg
    17161 gcgatatatt gccgctcaac gaaaaaggcg agcgtgtgtg gcctaaagct caggatgacg
    17221 ccagctttgt gctggtggac gcctcctgtt ccgccgaggc ggtggcgcgt atctcgccga
    17281 gaaccgcaac gttccataaa gggcaactgg tgtgggggaa tgtggcaggt tgatgcgcat
    17341 ttgatatgtc tgcaacggtg aacagtaaga gatttaagcc ccagggagtg gggcgggaat
    17401 ggtttgttaa tgaatgatcc tgaactttat aatctcccgg cataatttcc aggggattat
    17461 ttgagagaaa tacacaaaag tgagttttaa caaaggtatt tatcaagcca taaatgtgac
    17521 ctgcttcgga taaaaaagat taaacatagg aaaaccaatg atactcttgc tgatggttac
    17581 caacagtaat taagatgtga ttttttccgt aatcgaaaaa atattgccat gaccataaaa
    17641 gaaaaattct cacaaaaata tccccatgct tccttttgta cttttggcga ttcagcagcg
    17701 ttggccgatc acctcgcaac gttgattgcg actggtgtta aaacagcttc ctgtggttcg
    17761 ctggcgggat gtattgaaga caacgcattt ccgctgattg gtgagtataa gattgtagaa
    17821 aatagccgtg gtgagcctgt ctgtgttatc agagtgattg gcctgcattt actgcgcttt
    17881 tctgatgtca ccgcagagct tgcccgtaag gaaggtgaag gtgacctcag tcttgaatat
    17941 tggcgtaatg aacaccgtcg ttttttccag gcagaaggat gctattcacc agaaatggac
    18001 gttatctttg aagaatatgc ccttattgac gttgtgtaga taactatttt tctacgcgaa
    18061 ataagctcac aaacataaag tatcgctaat tttgtttatt ttgctgtttt ttacgtgatg
    18121 attgtcacac ttattgttgc tgcccgtttt gtccatattt ataaccaaat ttttaatagc
    18181 tgcctgaaat agggctgttt attctgcgta aaaatattcg aaaattctta ttctctccag
    18241 cgcgctaatt tgggcgaaca cttcctgact accctgcaat gaggctgaaa tgatgaataa
    18301 acgttttgtt atcaatatgg tgtcttcgct gttattaggc gctgcgctaa tttctgcgcc
    18361 gctccaggca gcggaaaaag tcgtggtgaa tatctctaaa gtcgatggta tgccgtggtt
    18421 taatcgcatg ggcgaaggtg tggttgaggc gggtaaagcc tttggtgtta atgcgtcaca
    18481 ggtggggcct tcaagtactg atgctccgca acaggttaaa attattgagg atttaatcgc
    18541 ccgtaaggtt aatgcaatta ctatcgtgcc aaacgatgcc aacgtgctgg aaccggtatt
    18601 caaaaaagcg cgagatgccg gaattgtggt tctgaccaac gaatctcccg gtcagccaag
    18661 tgctaactgg gatatcgaaa tcattgataa cgaaaaattt gccgctgaat atgttgagca
    18721 tatggcgaaa cgcatggggg gcaagggcgg ctatgtgatt tacgtgggta gtttgaccgt
    18781 gccgcagcat aacttatggg ctgacttgct ggtgaaatac cagaaagagc attacccgga
    18841 tatgcatgaa gttacccgcc gcatgccggt tgccgagagt gtggatgact cacgtcgtac
    18901 cacactggac ctgatgaaaa cctatcctga cttgaaagcg gttgtctcct ttggttcaaa
    18961 cggtccgatt ggcgcgggac gtgcagtgaa agagaaacgg gcgaaaaata aagtcgcagt
    19021 ctatggcatg atgatccctt cacaggcggc atcactgatt aaaagtggtg atatcaccga
    19081 agggattact tacgatccgg cctctgcagg ttacgcactc gcggccgtcg ccagcacgtt
    19141 gctgaagggt gaagagatca aacctggatt agaaatgcag aatctcggta aggccgacgt
    19201 agatatggat aagcgaatta tccgtttcca taaggtatta ctggtcaaca aagacaatat
    19261 cgactcactt tattaatcca tttcttcggg gcgaaagccc cgattcttat tgccagggga
    19321 tgctatggaa accttccttt cccttcgtca tatcaacaaa acgtttcatg ccacgcgagc
    19381 gctgcgcgat gtttctctcg actttatgtc gggtgaggtg cactgccttg ccgggcaaaa
    19441 cggctgtggt aaatcgacgt tgattaaaat tatgtccggt gtttatcgcc cggatgaggg
    19501 ggcggaaatt acgcttggtg ggaaaaactg gtcaaagctg acacccgccg cttcggtggc
    19561 gcaggggatt caggtgattt atcaggacct ctctttattt cctaacctga gtgtctggga
    19621 gaatatcgcc gtgaatcact atcaccacgg tctgtttgtt aaccgccgtc gtctgcgtga
    19681 ggtggcgcag gcggcaatga ccagtattaa cgtcacattg ccactggata cgctcgtttc
    19741 tgagctttca attgcccgct gtcagctggt ggcaatttgc cgtgcactgg cgcaggacgc
    19801 acggttgatc gtgatggatg aaccaaccgc ttcgctgacc catcaggaag tgcaagggtt
    19861 attacaggtg gtgcatcaat tgcgggaacg cgggatctgc gtggtcttcg tcagtcatcg
    19921 tctggaagaa gtgatggaag tttccgaccg tatttcagtg ctgaaagatg gtgagctggt
    19981 cgggaccttt ccggccgcag agatgaccac aaaacaactc agttttctga tgaccggcca
    20041 ggagttcgaa tatcaggtac gagagttgtg gcagggaaaa tccagcaccc cggtgctgga
    20101 ggtgcgtaat ttaagtcgtc atggggaata tcaaaatatt aacttgcggg tggaagcggg
    20161 cgaagtggtg tcgattgttg gcctgcttgg tgcggggcgc acggaattat gtctgagtct
    20221 gtttggtatg acccgaccgg atgctggtga gatcctcatc aacggtcagc cggttacact
    20281 gcatagtaac caggacgcca tccgccacgg tattggttat gtttcggaag atcgcatgtc
    20341 gcgcggtttg gtgatggcgc aatctattga agacaacatc attagcactg tttttcacaa
    20401 ggtaaaagat cgcttcggct ttctgagtga agctaaagtg cgtgacctgg tggacaggct
    20461 aatcaaggca ttgaccatca aagtctctga cccacatctc ccggtgaata cgctttccgg
    20521 gggtaacgcg cagcgagtat cgatcgccaa atggctggcg attgggccac gactgttgat
    20581 tctcgatagc ccgactgtcg gtgttgatat cgccaacaaa gcaggaatat acggcattat
    20641 cagcgatctg gcggctcatg gcattgcggt attgatgatc tgcgatgaaa ttgaagaagc
    20701 gtggtatcaa agccaccgga ttttggtaat gcaaaaaggt cagatcaccc atagcttcct
    20761 gcctgacagc agttctcagg ccaggattgc ggaggtggta aatggctgaa ctgaaaaaac
    20821 gccatgaatt ctggctggca ttactgattg tggtgctgtt tgttggcctt gcctggcgca
    20881 gcgacgagtt tctgacattc ggtaatttgt acgatctcgc caataactat gccatgttga
    20941 ctattctcgc ctgtggtttg tttgtggtgt taatttccgg tgggattgat atttcgtttc
    21001 cggcaatgac catcattgcg caatacggca tggtgctgtt gctgcaaaaa atgggtggca
    21061 actttgctgt cgcgtttgcc ctggcgggcg gcatcggcat tttacttggc ttaataaacg
    21121 ccttactggt taatcgccta cgggtgccgt ctatcatcat cactatctcg acgctgaata
    21181 ttttttatgg cctgctgtta tggttgagta aaggtgtgtg gctgtacgac tttccgccgt
    21241 ggtttgagca gggggtaatg ttgttcaagt acaccgatgc tgatggctat gactatggcc
    21301 ttggtctgcc gctgatcgcc atgattacgg tggtgctgct gacagcgttt atcatgaatt
    21361 tcaccagtgt agggcgcaaa atttatgccc ttggcgggaa ccgcgaatca gccagtcgca
    21421 tcggctttag tgtgctgaaa ctgcaacttt tcgtctatgg ctatatggga ttgatgtctg
    21481 gtgctgcggg tgtagtgcag tcgtggacgg tgatgactgt cgcccccgat tctattctgg
    21541 gttatgagct gacagtactg gctgcggtgg tgcttggtgg cactagtttg ctcggcgggc
    21601 gcggcacgtt aaccggtact ttgctcggcg tggtgttgtt ggcagtaatg caaaacgggc
    21661 taaatttatt gggagtctcg tcttactggc aaacattgat cacaggcatc atcatcgttg
    21721 ccagcattag cgccacggcg tggagtcagc atcagaaccg gagtctgcta tgaaaaaatc
    21781 gtggcgtaat aacgttgagt tttacttgat cggcctgctg gtgctaactg ttgccgcgtt
    21841 cagcattact atgccggaga ttttctggtc gataagtaat ttccagtccg tcgcttcgca
    21901 aatgcccgtg ctgggcattc tggcgttggc tatggcggtg accatgcttt gcggtggcat
    21961 taacttgtca attatagcca ctgctaacgc ctgttcactg gtgatggcgt gggtggcgac
    22021 tcagtatcca ccgggtattg ctacggttgt cgctacgctg ctggcgggcg ctggcgcggc
    22081 ggtgataatc ggtttatgta acggcgtatt gattgctggt attcgagtgt cgccaattct
    22141 tgcaacgctt gggatgatga cgttgctcaa aggggtaaat attctggtga caggcggtag
    22201 cgccattgcc aattatccct catgggtgct gtggttgaat catgcacagt ggttcggtat
    22261 tccgctaccg atgtggttgt ttaccgcagt ggcgctgggg ctgtggatat tgctggagaa
    22321 aacgccgctc ggtaaatcca tttatctgat tggttctaac gaacgagcaa cactttactc
    22381 cggtattaac actcgtcggg tgctgatatg ggtgtatgtc atttccgcct tgctttgtgc
    22441 ggtagcggca tttttgatga tgtcgaaact caactcggct aaggcctctt atggcgaatc
    22501 ttacttgcta gtgtcaatcc tcgcggcagt actcggcggg gttaatccgg acggcggcag
    22561 cgggcggata attggtatgg tgctggcgct gtttcttctg caaattattg aaagcggctt
    22621 caatattctc ggtataagtc cctacctgac gatggcctta tggggaacgt tgctgctctg
    22681 tttcattcag gctcgcggca tgttggggct ggatcgggtg gtttaaatat tggctatatt
    22741 caatggacgc gttttgccgc gatgacatat caggcatcgc caaatacaca tagctaatca
    22801 ggagtaaaca caatgaagat caaagctgtt ggtgcatatt cagctaaaca accacttgaa
    22861 ccgatggata tcacccggcg tgaaccggga ccgcatgatg tcaaaattga aatcgcttac
    22921 tgtggcgtct gccattccga tatccaccag gtccgttccg agtgggcggg gacggtttat
    22981 ccctgcgtgc cgggccatga aattgtagga cgcgtggtag ctgttggtga tcaggtagaa
    23041 aaatatgcgc cgggcgatct ggttggtgtc ggctgcattg tcgacagttg taaacattgc
    23101 gaagagtgtg aagacgggct ggaaaactac tgtgatcaca tgaccggcac ctataactcg
    23161 ccgacgccgg acgaaccggg ccatactctg ggcggctact cacaacagat cgtcgttcat
    23221 gagcgatatg ttctgcgtat tcgtcacccg caagagcacc tggcggcggt ggctcctttg
    23281 ttgtgtgcag ggatcaccac atactcgccg ctacgtcact ggcaggccgg gccgggtaaa
    23341 aaagtgggcg tggtcggcat cggcggtctg ggacatatgg ggatcaagct ggcccacgcg
    23401 atgggggcgc atgtggtggc atttaccact tctgagttaa aacgcgaagc ggcaaaagcc
    23461 ctgggggccg atgaagttgt taactcacgc aatgccgatg agatggcggc tcatctgaag
    23521 agtttcgatt tcattttgaa tacagtagct gcgccacata atctcgacga ttttaccacc
    23581 ttgctgaagc gcgatggcac catgacgttg gttggtgcgc ctgcgacacc gcataaatcg
    23641 ccggaagttt tcaacctgat catgaaacgc cgcgcgatag ccggctctat gattggcggc
    23701 attccagaaa cacaggagat gctcgatttt tgcgccgaac atggcatcgt ggctgatata
    23761 gagatgattc gggccgatca aattaatgaa gcctatgagc gaatgctgcg tggtgatgtg
    23821 aaatatcgtt ttgttatcga taatcgcaca ctaacagact gaaaaaatta ataaacatcc
    23881 tgtggtttaa catattaact tcgctccact taacttttta gttaaggaga gcgaaataat
    23941 atcaaagtag tggtaaacct atagcgtaaa ttcagattgt taaattaact ccactacttt
    24001 acacgttagt tatgagcgat ttctgatagt gtctggttta atcagagctt tattatctgc
    24061 gacgtttatt tttatttaat agagccacct gcaagttgtt attccaccag agagtgaatc
    24121 gcaaagcagg taatcatttc atctgtatat ctgtatgcca actggcattc catctctttt
    24181 ttcgccgata ttttgttgtg atcggcgaca cttcgcttaa aaaagcatca gcagtggttt
    24241 cgcagctatg cggtgtataa aaaatgatct catgcagatg ttttgtgaat gtgtgggttg
    24301 acattcatat gaaaaaaatc ataattccat catgtttgtg tggggtcttt tctgtatctt
    24361 acgcatcgca ctcaagcctg acagaaaata tgctgtaagg ctcatatcaa aaaccgccat
    24421 tagctcatca ggaagagcag acgacaacca ggattgttgt atggtacggg gttcgaggcc
    24481 tcgatggcag tccagctttt caaaactctt ctgaatcaga tccccttatt atcattcctg
    24541 caaagtggaa cattgccatt gtttggctat tcaggacggg tcagaccaat gaaaaaagcc
    24601 cacacagggg agagtgggct gaaatgggaa gctaaagact caagtaaact tatcggaaat
    24661 aaggaccacg cattacgggg gctatcatcg ccgatggtcg ggattcggca aatcaggttg
    24721 attacgtaga gagtaaatta tctgctcacc gctgcgtcac cccttcgtaa atcaggcgta
    24781 gcgcgaatac accaataatt gcaccaataa cccgactggc aacgcgttgc atacgcccat
    24841 aagcacgacg cactgcgggc aaagaaaacg cctgactaag aaaaacgcgc cagataactg
    24901 atgcgagcac aatccccgcc caggccatta aacgtgccca tgttggtgtt tcggcattta
    24961 atgttactga gaaaatacta ataaaaaata aaacggtttg cgggttagag agatcggtaa
    25021 ttaatccacg gcggaaaaag atataccagg gggcactaat cggttgttgt agtgtgctca
    25081 tttgcggtgt tgactggcgg cgcatgctgc accacgcaaa ccataagaga taagcgccgc
    25141 cgacgattct gataagcgaa aaaatctcct cacactgcgt aattagtgtt gcaagaccaa
    25201 acaaacccaa cccggaataa aatgcatcgc ccaacgccac gcccagtccg gtcagcaccc
    25261 ctgcgcgtcg accggaagcc aggctggttt gtactaccac aaagagattg gctcccggat
    25321 taaaaaaagt aatcacgaac agtcctacgg tcagataaac ggcgtgcaaa ggatccatag
    25381 tgatttcaac cataaataag tgaagtaact tcatcatatt tctaccaaaa ataatcgggt
    25441 gcgagagaga tcacaaagtg tcttatttcc ggttactggc gtttatgccc tgactgaact
    25501 aattattaat caacccaata atgtgggtgg gtgatagtgt gataacaact ctggagccgt
    25561 aatatgaaaa taatctctaa aatgttagtc ggtgcgttag cgtttgccgt taccaatgtc
    25621 tatgccgcag aattaatgac caaagcggaa tttgaaaagg ttgcatcgca atatgaaaaa
    25681 ataggtgata tttcaaccag taatgaaatg tcgaccgcag atgcgaaaga agatttgatc
    25741 aaaaaagccg atgaaaaagg ggctgatgtg ttggtactga cctctggtca aactgacaat
    25801 aagatccacg gcacggcaaa tatttataag aagaagtaat tctgaatcct atgtaaacat
    25861 ccccgatgcg taagtttatc ggtgatcatc atctattgaa atttatgccg gataaagcgt
    25921 tcgcgctgca ttcggcagtt cagcttttca gccgccgcca gaacgtcgtc cggctgatac
    25981 ctaaataatt cgccgctgct gttttatcgc cattaaattt ctccagtgcc tgttgtgctg
    26041 tcagcaagcc tggaatggga gtcttcgccg actcgcgtgc cagttccggc agtagcagct
    26101 gcaaaaattg cggcgttaaa tccggcgtcg attccacact taaaaacagc gccagtcgtt
    26161 ccatcatatt gcgcagttca cgaatattgc ccggccagtc gtagtgcacc agcatggttt
    26221 cgctttcctg taatccctgg cgtaatgcgg cagcaaacgg ggcggagagc gccgccagag
    26281 acactttcaa aaaactttcc gccagcggca gaatatccgc cacccgctcg cgcagtggtg
    26341 gcaattgcag acgcaaaata ctcagccgat aaaacagatc acggcgaaac tgcccttgcc
    26401 gcatatcttc ttccagattg cagtgagtgg cgctaatgac tcgcacatcc accggaacag
    26461 gctgatgccc gccgacgcgg gtgacctctt tttcttccag cacccgcagc agccgagtct
    26521 gcaacggcag cggcatttcg cctatctcgt caagaaacag cgtgccaccg tgggcgattt
    26581 cgaacagccc ggcgcgacct ccgcgtcgcg agccggtaaa cgccccttcc tcatggccaa
    26641 acagctccgc ttccagcagc gattcggcaa tcgctccgca gttgacggca acaaacggat
    26701 gcgacttttt gccctgtcgc gcatcgtggc gggcaaaata ttcccgatga atcgcctggg
    26761 ccgccagctc tttgcccgtc cccgtttccc cctcaatcaa caccgccgca ctggagcggg
    26821 catacagcaa aatagtctgc cgcacctgtt ccatctgtgg tgattgaccg agcatatcgc
    26881 ccagcacgta acgagtacgc agggcgttgc gggtggcatc gtgagtgtta tggcgtaacg
    26941 acatgcgcgt catatccagc gcatcgctga atgcctggcg cacggtggcg gcggaataga
    27001 taaaaattcc ggtcattccg gcttcttctg ccagatcggt aatcagcccc gcgccgacca
    27061 ccgcttcggt gccgttagct ttcagctcgt taatctgccc gcgcgcgtct tcttcggtaa
    27121 tgtagctacg ttgatcgagg cgcaaattaa aggttttttg aaacgccacc agtgccggaa
    27181 tagtttcctg ataagtgaca acgccgatag aagaggtgag ctttccggct tttgccagag
    27241 cctgtaacac atcgtagccg ctcggtttaa tcaaaataac cggcactgac aggcgacttt
    27301 taagatatgc gccgttagag ccagcagcga tgatggcgtc gcagcgttcg tttgccagtt
    27361 ttttgcggat gtaggtcacc gctttttcaa agccaagctg aataggggtg atattcgcca
    27421 ggtgatcaaa ctcgagactg atatcgcgaa acagctcgaa cagccgcgtt acagataccg
    27481 tccagatcac cggtttgtcg tcattcagcc gtggtggatg tgccatagcg caccgcaaag
    27541 ttaagaaacc gaatattggg tttagtcttg tttcataatt gttgcaatga aacgcggtga
    27601 aacattgcct gaaacgttaa ctgaaacgca tatttgcgaa ttacttcatg actatattgc
    27661 taacaaattg aaattaaaca tttaatttta ttcagtcaat tgtggcacac cccttgcttt
    27721 gtgtttatca acgcaaataa caagttgata acaaaggatg ggctatgtat ctacactctc
    27781 caggtaaagc gtttcgcgct gcactgtcta aagaaaatcc attgcagatt gttggcacca
    27841 tcaacgctaa ccatgcgctg ctggcgcagc gtgccggata tcaggcaatt tatctctccg
    27901 gcggtggcgt ggcggcaggg tcgctggggc tgcccgatct cggtatttct actcttgatg
    27961 acgtgctgac agatattcgc cgtatcaccg acgtttgttc gctgccgctg ctggtggatg
    28021 cggatatcgg ttttggttct tctgctttta acgtggcgcg caccgtgaaa tcgatgatta
    28081 aagccggtgc ggcaggattg catattgaag atcaggttgg tgcgaaacgc tgcggtcatc
    28141 gtccgaataa agcgatcgtc tcgaaagaag agatggtgga tcggatccgc gcggcggtgg
    28201 atgcgaaaac cgatcctgat tttgtgatca tggcgcgcac cgatgcgctg gcggtagagg
    28261 ggctggatgc ggcgattgag cgtgcgcagg cctatgttga agcgggtgcc gagatgttgt
    28321 tcccggaggc aattaccgaa ctcgccatgt accgccagtt tgccgatgcg gtgcaggtgc
    28381 cgatcctcgc caacatcacc gaatttggcg caacaccgct gtttaccacc gacgaattac
    28441 gcagcgccca tgtcgcaatg gcgctgtacc cactttcagc gttccgcgcc atgaaccgcg
    28501 ccgctgagca tgtctacaac gtcctgcgtc aggaagggac gcagaaaagc gtcatcgaca
    28561 ccatgcagac ccgcaacgag ctgtacgaaa gcatcaacta ctaccagtac gaagagaagc
    28621 tcgacgacct gtttgcccgt ggtcaggtga aataaaaacg cccgttggtt gtattcgaca
    28681 accgatgcct gatgcgccgc tgacgcgact tatcaggcct acgaggtgaa ctgaactgta
    28741 ggtcggataa gacgcatagc gtcgcatccg acaacaatct cgaccctaca aatgataaca
    28801 atgacgagga taatatgagc gacacaacga tcctgcaaaa cagtacccat gtcattaaac
    28861 cgaaaaaatc ggtggcgctt tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg
    28921 gtaaaagcgg caatgatttg cattaccgcg gctacgacat tcttgatctg gcggaacatt
    28981 gcgaatttga agaagtggcg catctgctga tccacggcaa actgccgacc cgtgacgaac
    29041 tcgccgccta caaaacgaaa ctgaaagccc tgcgcggttt accggctaac gtgcgtaccg
    29101 tgctggaagc cttaccggcg gcgtcacacc cgatggatgt tatgcgcacc ggcgtttccg
    29161 cgctcggctg cacgctgcca gaaaaagagg ggcataccgt ctctggcgcg cgggatattg
    29221 ccgacaaact gctggcgtca cttagctcga ttcttctcta ctggtatcac tacagccaca
    29281 acggcgaacg cattcagccg gaaaccgatg acgactccat cggcggtcac ttcctgcatc
    29341 tgctgcacgg cgaaaagccg tcgcaaagct gggaaaaggc gatgcatatt tcgctggtgc
    29401 tgtacgccga acacgagttt aacgcctcca cctttaccag ccgggtgatt gcgggcaccg
    29461 gctctgatat gtattccgcg attattggcg cgattggtgc actgcgcggg ccgaagcacg
    29521 gcggggcgaa tgaagtgtcg ctggagatcc agcaacgcta cgaaacgccg gacgaagccg
    29581 aagccgatat ccgcaagcgg gtggaaaaca aagaagtggt cattggtttt ggtcatccgg
    29641 tttataccat cgccgacccg cgtcatcagg tgatcaaacg tgtggcgaag cagctctcgc
    29701 aggaaggcgg ctcgctgaag atgtacaaca tcgccgatcg cctggaaacg gtgatgtggg
    29761 agagcaaaaa gatgttcccc aatctcgact ggttctccgc tgtttcctac aacatgatgg
    29821 gtgttcccac cgagatgttc acaccactgt ttgttatcgc ccgcgtcact ggctgggcgg
    29881 cgcacattat cgaacaacgt caggacaaca aaattatccg tccttccgcc aattatgttg
    29941 gaccggaaga ccgccagttt gtcgcgctgg ataagcgcca gtaaacctct acgaataaca
    30001 ataaggaaac gtacccaatg tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg
    30061 aaatcgttga tatcgtcgat tacgtgatga actacgaaat cagctccaaa gtagcctacg
    30121 acaccgcaca ttactgcctg ctcgacacgc tcggctgcgg tcttgaagct ctcgaatacc
    30181 cagcctgtaa aaaactgctg gggccaattg tccccggcac cgtcgtacct aacggcgtgc
    30241 gcgtccccgg aactcagttc cagctcgacc ccgtccaggc ggcatttaac atcggcgcga
    30301 tgatccgttg gctcgatttc aacgatacct ggctggcggc ggagtggggc catccttccg
    30361 acaacctcgg cggcattctg gcaacggcag actggctttc gcgcaacgcg gtcgctagcg
    30421 gcaaagcgcc gttgaccatg aagcaggtgc tgaccgcaat gatcaaagcc catgaaattc
    30481 agggctgcat cgcgctggaa aactccttta accgcgtcgg cctcgaccac gttctgttag
    30541 tgaaagtggc ttccaccgcc gtggtcgccg aaatgctcgg cctgacccgc gaggaaattc
    30601 tcaacgccgt ttcgctggcg tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc
    30661 cgaacaccgg cacgcgtaaa tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc
    30721 tggcactgat ggcgaaaacg ggcgaaatgg gttatccgtc agccctgact gcgccagtat
    30781 ggggtttcta cgacgtctcc tttaaaggtg agtcattccg cttccagcgt ccgtacggtt
    30841 cctacgtcat ggaaaatgtg ctgttcaaaa tctccttccc ggcggagttc cactcccaga
    30901 cggcagttga agcggcgatg acgctctatg aacagatgca ggcagcaggc aaaacggcgg
    30961 cagatatcga aaaagtgacc attcgcaccc acgaagcctg tattcgcatc atcgacaaaa
    31021 aagggccgct caataacccg gcagaccgcg accactgcat tcagtacatg gtggcgatcc
    31081 cgctgctgtt cggacgctta acggcggcag attacgagga caacgttgcg caagataaac
    31141 gcatcgacgc cctgcgcgag aagatcaatt gctttgaaga tccggcgttt accgctgact
    31201 accacgaccc ggaaaaacgc gccatcgcca atgccataac ccttgagttc accgacggca
    31261 cacgatttga agaagtggtg gtggagtacc caattggtca tgctcgccgc cgtcaggatg
    31321 gcattccgaa gctggtcgat aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc
    31381 agcagcagcg cattctggag gtttctctcg acagaactcg cctggaacag atgccggtca
    31441 atgagtatct cgacctgtac gtcatttaag taaacggcgg taaggcgtaa gttcaacagg
    31501 agagcattat gtcttttagc gaattttatc agcgttcgat taacgaaccg gagaagttct
    31561 gggccgagca ggcccggcgt attgactggc agacgccctt tacgcaaacg ctcgaccaca
    31621 gcaacccgcc gtttgcccgt tggttttgtg aaggccgaac caacttgtgt cacaacgcta
    31681 tcgatcgctg gctggagaaa cagccagagg cgctggcatt gattgccgtc tcttcggaaa
    31741 cagaggaaga gcgtaccttt accttccgcc agttacatga cgaagtgaat gcggtggcgt
    31801 caatgctgcg ctcactgggc gtgcagcgtg gcgatcgggt gctggtgtat atgccgatga
    31861 ttgccgaagc gcatattacc ctgctggcct gcgcgcgcat tggtgctatt cactcggtgg
    31921 tgtttggggg atttgcttcg cacagcgtgg caacgcgaat tgatgacgct aaaccggtgc
    31981 tgattgtctc ggctgatgcc ggggcgcgcg gcggtaaaat cattccctat aaaaaattgc
    32041 tcgacgatgc gataagtcag gcacagcatc agccgcgtca cgttttactg gtggatcgcg
    32101 ggctggcgaa aatggcacgc gtcagcgggc gggatgtcga tttcgcgtcg ttgcgccatc
    32161 aacacatcgg cgcgcgggta ccggtggcgt ggctggaatc caacgaaacc tcctgcattc
    32221 tctacacctc cggcacgacc ggcaaaccta aaggcgtgca acgtgacgtt ggcggatatg
    32281 cggtggcgct ggcgacctcg atggacacca tttttggcgg caaagcgggc ggtgtgttct
    32341 tttgtgcttc ggatatcggc tgggtggtgg ggcattcgta tatcgtctac gcgccgctgc
    32401 tggcggggat ggcgactatc gtttacgaag gattaccgac ctggccggac tgcggcgtgt
    32461 ggtggaaaat cgtcgagaaa tatcaggtta gccgcatgtt ctcagcgccg accgccattc
    32521 gcgtgctgaa aaaattccct accgctgaaa ttcgcaaaca cgatctctcg tcgctggaag
    32581 tgctctatct ggcgggggag ccgctggacg agccaaccgc cagttgggtg agcaatacgc
    32641 tggatgtgcc ggtgatcgac aattactggc agaccgaatc cggctggccg attatggcga
    32701 ttgctcgcgg tctggacgac aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg
    32761 gctataacgt gcagttgctc aatgaagtca ccggcgaacc gtgcggcgtc aacgagaagg
    32821 ggatgctggt ggtggaaggg ccgctgccgc cggggtgtat tcagaccatc tggggcgatg
    32881 acgaccgctt tgtgaagacg tactggtcgc tgttttcccg tccggtgtat gccacctttg
    32941 actggggcat ccgcgatgct gacggttatc actttattct cgggcgcact gacgatgtga
    33001 ttaacgttgc cgggcatcgg ctggggacgc gcgagattga agagagtatc tccagtcatc
    33061 cgggcgttgc cgaagtggcg gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg
    33121 tggcgtttgt cattccgaaa gagagcgaca gtctggaaga ccgcgaggtg gcgcactcgc
    33181 aagagaaggc gattatggcg ctggtggaca gccagattgg caactttggc cgcccggcgc
    33241 acgtctggtt tgtctcgcaa ttgccgaaaa cgcgatccgg aaaaatgctg cgccgcacga
    33301 tccaggcgat ttgcgaagga cgcgatcctg gggatctgac gaccattgat gatccggcgt
    33361 cgttggatca gatccgccag gcgatggaag
//
LOCUS       DAZGTJ010000053        32867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000053 DAZGTJ010000000
VERSION     DAZGTJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.053"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            57..1655
                     /gene="rtcR"
                     /locus_tag="ACHK8E_003010"
     CDS             57..1655
                     /gene="rtcR"
                     /locus_tag="ACHK8E_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP0345246.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1637..2395)
                     /locus_tag="ACHK8E_003011"
     CDS             complement(1637..2395)
                     /locus_tag="ACHK8E_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP0345247.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2412..3242)
                     /gene="glpG"
                     /locus_tag="ACHK8E_003012"
     CDS             complement(2412..3242)
                     /gene="glpG"
                     /locus_tag="ACHK8E_003012"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP0345248.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3287..3613)
                     /gene="glpE"
                     /locus_tag="ACHK8E_003013"
     CDS             complement(3287..3613)
                     /gene="glpE"
                     /locus_tag="ACHK8E_003013"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP0345249.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            3803..5308
                     /gene="glpD"
                     /locus_tag="ACHK8E_003014"
     CDS             3803..5308
                     /gene="glpD"
                     /locus_tag="ACHK8E_003014"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP0345250.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5362..5967)
                     /locus_tag="ACHK8E_003015"
     CDS             complement(5362..5967)
                     /locus_tag="ACHK8E_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345251.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(5970..6746)
                     /locus_tag="ACHK8E_003016"
     CDS             complement(5970..6746)
                     /locus_tag="ACHK8E_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345252.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(6749..7459)
                     /locus_tag="ACHK8E_003017"
     CDS             complement(6749..7459)
                     /locus_tag="ACHK8E_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345253.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            complement(7633..8754)
                     /locus_tag="ACHK8E_003018"
     CDS             complement(7633..8754)
                     /locus_tag="ACHK8E_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345254.1"
                     /translation="MITYAGYKVKIIFIIFLFLGYHVVAYAEMGRDREFCYPGTPGNN
                     TTPATFFYDFGTVIISDVNNNSAGTVLPSQNWKIGRYKAYCNSLDDYEVYFSGISGID
                     PSGASGNPQGAEIFIPVTHEISISTHIKLYNQNGTMTDKTVPFENYSTNYPADRSKAD
                     YWSSGTEGYIKIRLDKKIISDVSLNNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQV
                     PQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTL
                     VFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQ
                     AASAQTADVTFLALPVSTTGKVPAAGRYNALAVLRVEYQ"
     gene            complement(8769..9548)
                     /locus_tag="ACHK8E_003019"
     CDS             complement(8769..9548)
                     /locus_tag="ACHK8E_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0345255.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGSQFKTD"
     gene            complement(9514..10086)
                     /locus_tag="ACHK8E_003020"
     CDS             complement(9514..10086)
                     /locus_tag="ACHK8E_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345256.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(10037..10600)
                     /locus_tag="ACHK8E_003021"
     CDS             complement(10037..10600)
                     /locus_tag="ACHK8E_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345257.1"
                     /translation="MNLIFSKYSVFFILFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(10600..13194)
                     /locus_tag="ACHK8E_003022"
     CDS             complement(10600..13194)
                     /locus_tag="ACHK8E_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0345258.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(13231..13983)
                     /locus_tag="ACHK8E_003023"
     CDS             complement(13231..13983)
                     /locus_tag="ACHK8E_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0345259.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(14084..14788)
                     /locus_tag="ACHK8E_003024"
     CDS             complement(14084..14788)
                     /locus_tag="ACHK8E_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345260.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
     gene            complement(15280..17727)
                     /gene="glgP"
                     /locus_tag="ACHK8E_003025"
     CDS             complement(15280..17727)
                     /gene="glgP"
                     /locus_tag="ACHK8E_003025"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP0345261.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(17746..19179)
                     /gene="glgA"
                     /locus_tag="ACHK8E_003026"
     CDS             complement(17746..19179)
                     /gene="glgA"
                     /locus_tag="ACHK8E_003026"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP0345262.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(19179..20474)
                     /gene="glgC"
                     /locus_tag="ACHK8E_003027"
     CDS             complement(19179..20474)
                     /gene="glgC"
                     /locus_tag="ACHK8E_003027"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP0345263.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(20492..22465)
                     /gene="glgX"
                     /locus_tag="ACHK8E_003028"
     CDS             complement(20492..22465)
                     /gene="glgX"
                     /locus_tag="ACHK8E_003028"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP0345264.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(22462..24648)
                     /gene="glgB"
                     /locus_tag="ACHK8E_003029"
     CDS             complement(22462..24648)
                     /gene="glgB"
                     /locus_tag="ACHK8E_003029"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP0345265.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24921..26024)
                     /gene="asd"
                     /locus_tag="ACHK8E_003030"
     CDS             complement(24921..26024)
                     /gene="asd"
                     /locus_tag="ACHK8E_003030"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0345266.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            26216..26809
                     /gene="yhgN"
                     /locus_tag="ACHK8E_003031"
     CDS             26216..26809
                     /gene="yhgN"
                     /locus_tag="ACHK8E_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP0345267.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26866..28206)
                     /gene="gntU"
                     /locus_tag="ACHK8E_003032"
     CDS             complement(26866..28206)
                     /gene="gntU"
                     /locus_tag="ACHK8E_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0345268.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(28210..28737)
                     /gene="gntK"
                     /locus_tag="ACHK8E_003033"
     CDS             complement(28210..28737)
                     /gene="gntK"
                     /locus_tag="ACHK8E_003033"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0345269.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(28876..29871)
                     /gene="gntR"
                     /locus_tag="ACHK8E_003034"
     CDS             complement(28876..29871)
                     /gene="gntR"
                     /locus_tag="ACHK8E_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP0345270.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(30095..30790)
                     /gene="yhhW"
                     /locus_tag="ACHK8E_003035"
     CDS             complement(30095..30790)
                     /gene="yhhW"
                     /locus_tag="ACHK8E_003035"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP0345271.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(30913..31950)
                     /locus_tag="ACHK8E_003036"
     CDS             complement(30913..31950)
                     /locus_tag="ACHK8E_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0345272.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            32284..32772
                     /gene="yhhY"
                     /locus_tag="ACHK8E_003037"
     CDS             32284..32772
                     /gene="yhhY"
                     /locus_tag="ACHK8E_003037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0345273.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         8594 a         8427 c         7693 g         8153 t
ORIGIN      
        1 tcccgcaaag ccgatttacc gataatgaaa tctcgtcttt tataaggata tctaagatgc
       61 gtaaaacagt ggcttttggc tttgtcggaa cggtgctgga ttatgccggg cgcggcagtc
      121 agcgctggtc gaaatggcgt ccgtcactct gtctatgcca gcaagaatcg ttagtcatca
      181 atcgactgga attgttgcac gacgcccgct cgcgctcgct gtttgaaacg cttaaatgcg
      241 atatcgccag cgtttcgcca gaaactgaag tggtgagcgt tgagattgaa ctgcataacc
      301 cgtgggattt cgaagaggtt tacgcctgcc tgcatgattt cgcccgtggc tacgcgtttc
      361 aacctgataa agaagactat ttaattcaca tcaccactgg cacccacgtc gcacagattt
      421 gctggtttct gctggcggaa gcgcgttacc tgcccgcccg gctgatacaa tcttccccac
      481 cgcgcaaaaa agaacagccg cgcggcgcag gtgaagtgac gattatcgat ctcgatttaa
      541 gccgttataa cgccatcgcc agccgctttg ccgaggaacg gcagcaaacg ctcgattttc
      601 ttaagtccgg cattgccacg cgtaaccccc acttcaaccg catgattgag cagattgaaa
      661 aagtggcgat taaatcccgc gcaccgatcc tgcttaacgg cccgacaggt gcgggcaagt
      721 catttctggc acgacgcatc ttcgaattaa aacaggcacg gcatcagttt agcggcgcat
      781 ttgtagaaat gaactgcgcc actctgcgtg gcgataccgc catgtcgacg ctatttggtc
      841 atgtgaaagg cgcgtttacc ggggcgcggg agtcgcggga aggattatta cgcagcgcca
      901 acggcgggat gttatttctt gatgagattg gcgaactggg cgcagacgaa caggcaatgc
      961 tgctgaaagc cattgaagag aaaacctttt atccgtttgg cagcgatcgc caggtgagta
     1021 gcgactttca gcttatcgcc ggaacggtgc gcgatttgcg tcagctggtt gccgaaggca
     1081 aattccgcga agatctgtac gcgcggatca atctctggac cttcaccttg ccggggctga
     1141 gccagcgcca ggaagatatt gaaccgaacc ttgattatga agtggagcgc cacgcctcac
     1201 tcactggcga cagcgtgcgt tttaacaccg aagcgcggcg cgcctggctg gcttttgcga
     1261 cctctcccca ggcaacatgg cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga
     1321 tggcaacctt tgccactagc ggacgcatca ctcgggaagt ggtggaagat gagataaacc
     1381 gtctgcgcta taactggcag gagagtcgcc cctccgcgat tacggcgttg ctgggcgcag
     1441 aggcagaaaa catcgatctc ttcgaccgta tgcaactgga acacgtaata gccatctgcc
     1501 gccaggcaaa gtcactttct gctgccggtc gccagctttt tgatgtttcg cgccagggca
     1561 aagccagcgt caacgacgca gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt
     1621 gggaagccgt gcaggatcag cacagctcca gttgaatatg atggtccgtc agcacctgca
     1681 tgacgcttac tggcggcggg gcgtcggtgt agacagcatc aaccatgctg atgctcccca
     1741 tattgaccat cgcattacgg ccaaatttcg agtgatcgac aaccagcata acgtggcgcg
     1801 agttctcaat aatggcgcgt ttggtgcgaa cttcgtggta atcgaattcc agcaacgagc
     1861 cgtcgctatc gatgccgctg atccccagaa tgccgaaatc aaggcggaac tgggagataa
     1921 aatcgagcgt cgcttcgcct atgatcccac catcgcggct acgtaattcg ccaccggcga
     1981 gaataatgcg aaaatcctct tttaccatca gcgtgttagc gacgttaaga ttgttggtga
     2041 caatgcgcaa attgctgtga ttgagcagcg cgtgcgccac cgcttccggg gtggtgccga
     2101 tatcgataaa cagcgttgag ccattaggaa tttgctccgc cactttgcgg gcgatgcgct
     2161 ctttttcttc ggtctgggtg gctttacgat cgtgccatgg cgtgttaacc gaactggaag
     2221 gcagcgccgc accgccatga tggcgcagga tcaggttttg ctctgccagc tcgttgaggt
     2281 cgcgacgaat agtctgcggg ctgacggaga agtgctccac cagttcctcg gtactgacat
     2341 aaccctgctg tttaaccagt tcgataatac cgttgtgacg ttgtgtttgt ttcatttata
     2401 aatccctgga attattttcg ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa
     2461 cccacggcta acccggcgat gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac
     2521 catccggcga caatccagat cagcgcaaag ataattaacc cacgttgcag gtaaatgcca
     2581 ctttgcggat cgcgttcgcc acgtagccag acgtagccca tcagcgcata caccacgcca
     2641 gaaagcccgc caaaccacgg cccgctgaat ttttgctgca catagccgct taacagggcg
     2701 ctaatgagag taatgacaat tagcttaccg ctaccgaggc gtttttccac cgcaccgccg
     2761 agataccacc accagagcag gttaaagagg atatgcatca gcgagaagtg cattaacgcg
     2821 tgggtgaagt aacgccagaa ctcaaatttc agtgttggat cgaatggcca ggccagccat
     2881 aacatcactt cctgatcgcc gagaatttgc atggcaataa acaccaccac gcaggcgatc
     2941 atcatcaccc aggttaccgg acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg
     3001 cgataatgca ggccactgcc ggtatggccc gcctgccagc tggccgccag ataacgcgga
     3061 tctgccgggt tttcgagaaa acgcgccagc tctgcccgca cgcgctcggc ctgggactca
     3121 tccgccagcc agacatcgct ttggttatgt tgttgaatcg tgaggataac accctgcgtc
     3181 gccatgtaat caacaaacgc ctgcgccacg cgggggttag caaaagaggt aatcatcaac
     3241 atcgttgctg tcgcttattc cacacaaaag gggacagtat aaagcgttac gcgccgtacg
     3301 ccacctctgc aggaaactga cgccgccagg cttcaaagcc gccgtcaatg ctatagacca
     3361 catcgtagcc ctgttgcagc agatactgcg ccgcgccttt gctgctattg ccgtgataac
     3421 acatcaccat caccggtgtg tcaaagtcgt tatcacgcat aaacgcgccc agtgtgtcgt
     3481 tagttaaatg gaaagcctgc accgcatgcc ccattgcgaa actctgtgga tcgcgaatat
     3541 cgaccagcac cgcctctttt tcctgcaact tctggtgcgc gacggcaacg ttaatacatt
     3601 cgaactgctc catgcgtctc tctttcttta caaacaagtg ggcaaattta ccgcacagtt
     3661 tacgtcgaag cggcagataa acgccataat gttatacata tcactctaaa atgttttttc
     3721 aatgttacct aaagcgcgat tctttgctaa tatgttcgat aacgaacatt tatgagcttt
     3781 aacgaaagtg aatgagggca gcatggaaac caaagatctg attgtgatag gtggcggcat
     3841 caatggtgct ggtatcgcgg cagacgccgc tggacgcggt ttatccgtgc tgatgctgga
     3901 ggcgcaggat ctcgcttgcg cgacctcttc cgccagttca aaactcattc acggtggcct
     3961 gcgctacctt gagcactatg aattccgcct ggtcagcgag gcgctggctg aacgtgaagt
     4021 gctactgaaa atggccccgc atatcgcctt cccgatgcgt tttcgcctgc cgcatcgtcc
     4081 gcatctgcgc ccggcgtgga tgattcgcat tggtctgttt atgtacgatc atctgggtaa
     4141 acgcaccagc ctgccgggat caaccggttt gcgttttggc gcaaattcag tgttaaaacc
     4201 ggaaattaag cgcggattcg aatattccga ctgttgggta gacgacgccc gtctggtact
     4261 cgccaacgcc cagatggtag tgcgtaaagg cggtgaagtg cttactcgta ctcgtgccac
     4321 ttctgctcgc cgcgaaaacg gcctgtggat tgtggaagcg gaagatatcg ataccggcaa
     4381 aaaatatagc tggcaagcgc gcggcctcgt gaacgccacc ggcccgtggg taaagcagtt
     4441 cttcgacgac gggatgcatc tgccttcgcc ttatggcatt cgcctgatca aaggcagcca
     4501 tattgtcgtg ccgcgcgttc atacacaaaa gcaggcctac attctgcaaa acgaagataa
     4561 acgtattgtc ttcgtgatcc cgtggatgga cgagttttcc atcatcggca ccaccgatgt
     4621 ggaatacaaa ggtgatccaa aagcagtgaa gattgaagag agtgaaatca attacctgct
     4681 gaaagtgtat aacacgcact ttaaaaagca gttaagccgg gacgatatcg tctggactta
     4741 ctccggcgtg cgtccgctgt gtgatgatga gtccgattcg ccgcaggcta tcactcgtga
     4801 ttacaccctt gatatccatg atgaaaacgg caaagcgccg ctgctgtcgg tattcggcgg
     4861 taagctgacc acctaccgca aactggcgga gcacgcgctg gaaaaactga cgccatatta
     4921 tcagggcatt ggtccggcgt ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg
     4981 cgaccgcgac gattatgccg ctcgcctgcg ccgccgctat ccgttcctga ctgaatcgct
     5041 ggcacgccat tacgcgcgca cgtacggcag caacagcgag ttgctgctcg gcaatgcggg
     5101 gacggtaagc gatctcgggg aagatttcgg tcatgagttc tacgaagcgg agctgaaata
     5161 cctggtggat cacgaatggg tccgccgcgc cgacgatgcc ctgtggcgtc gcacaaaaca
     5221 aggcatgtgg ctgaatgcgg aacaacaatc tcgtgtgagt cagtggctgg tggagtatac
     5281 gcagcagaag ttgtcgctgg cgtcgtaagg atgatgcccg gttgtgaagt gcaaccgggc
     5341 tatttggatt ccttagacta ttcattgtaa ataattgaat aacggcccct gagctaaaat
     5401 cgccaaactt aagcacgttc cccactcaac ccacttctgc ccctggcttc tatttttgct
     5461 acgccactgg cagtaatatc ccaacgcaaa accaagctgt gcactaatcg ggccgacaaa
     5521 atcagtccac ggctgcggcc aaaaagggga aaaattcagg ggaagccaga caaacgtcag
     5581 aaagaagttt cggatatttt ccagcaacgc tgatttctct ggagtaatat aaagaatgaa
     5641 attcgagaga attaaccaga aataggccgg agaaaagacc agccagtaat gcagtctgtt
     5701 ttttaattca ggaggcgttc tgcgggagaa ttgccaaccg atgccaccca ttacactgaa
     5761 aaaaagtgca tattcaacca ttaactcaag ttgtaattcc tgaagagaaa tacgcacaac
     5821 caggagcgga aaggagaata ttatttcagc cagaaaagtt aacgtgaata taagtacggc
     5881 taaggcaata accgtcggcc ataccaataa tggcacgata acaaaaccga gaattacagg
     5941 gggaatataa agccggaatt taatcatttt tattttcctg gtgattcgga tgatgcgtca
     6001 tacatcgcgc ataaacactc atcaatcatc cagtcgtctt cccagacctg ataatgtgtg
     6061 ccctgttcgg gctgattaag caggttctca atcgcctgcc gcgcacgttc tttcaggatg
     6121 aattgattgt tgaaggccca ggcattgatt tgggcggtaa tggctttatc gtcatcatcc
     6181 tgctcaaaac gactattctg aatattctct tccctgttat gccacagcaa acatgccatc
     6241 gatactgtgg gttggtaacc gggtacagag atgatggcat caagcaatgg cgaaagagcg
     6301 atatcggcac gggaaaacat ttccagcata tactgtaatt cgacagcctc atcgccagat
     6361 acccaatcag ccatatactg gcaggcaaat cgctgcaata ttgcccgttc atcctcacgc
     6421 cagttttctt tactggcaag gttgatacgc cgtagagaaa atacttcgtg aatattgtca
     6481 aattgcccct gcgcaacaaa ctccagtaaa cgaggaagaa aataacgaac ctcatcgctg
     6541 ttttgcggat caggtcctgg actactattc caggcattga ttaaggaata aggaatcgct
     6601 cgcaacgatg tattccgtaa tgctttttgt tcctgttgac tcaggcaata ttcacaacaa
     6661 accacaaatt gctgcggcac cggataacga cgaaaaatat gataaacctg ctcaattaat
     6721 gcctgcatgg cggagctggg ttgcataatt accccctctt ccgctggtcg cgtgtaaacg
     6781 gttgataaac aaaacgagca ttcgccgcat tcaggcgaat aatggaacta cgagagaggg
     6841 aaaagctggg ccgaatgcaa taattacaat cccgacagct ttcctctgtg caattttcca
     6901 ctgagtaatg acgcgcgtag agtttcagtt cagcctgctt atcccgaaaa ccgcgtaagc
     6961 tgttatcggt atggaatttc catgcttctt taaacacctg aaagatataa acatcctcaa
     7021 tccaggagtc acatttttta agcgggcgct ttgctatgat cacccgctca tccggattga
     7081 gcattgccgt aaaatgaacg gtaaagtcat ttgtcgtatt caggtcacat tgaacatctg
     7141 gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa agacgaccga
     7201 tcgtggcggt atcgagggaa aatcccggcc atacattgct ggaggttaag ccaatattta
     7261 atgtgacatc atatcgagat aatccctcaa gaccgacagt accggcgaac ttttcccctt
     7321 catccaggac ctggttaatg agttgctgaa taatgccttc atcccagtat gttgatttgc
     7381 agttggcaat cgtaatatcc acgccagtga atggatcagt accggagagg cttgcaaata
     7441 acgtatcaac taattccatt tttattatcc ggcttaatgc aacattttat ccatcggcac
     7501 ataacctaca ttttccacca gattttgtcc ctgtagaatt aaaaatattt ttttgtttta
     7561 cattctgttc atccctatta ttgcatttta ttttgcggtg atggctaaac cgattctgta
     7621 ctagctcaaa aattactgat attccactcg caatacagcc agtgcattat aacgacctgc
     7681 cgcaggcact tttccagttg tactaacagg taaagccagg aaagtaacat cagcagtttg
     7741 cgcagaagca gcctggtttt ccaccaatct gaacggaacg cctactgatg ttgccagagc
     7801 atttaaatct acggagtttt gctgactatc cataatcata ataccaacat caggattatc
     7861 ttgtgcctga ataatacttt gtttcccatt ggtatcacgt gtagctgaaa tgttaccgtc
     7921 gaataccaac gtcattaccg catctgtatc tttattcgta caattaatcg gaatagttgt
     7981 tgctacagga gtaacaccag caggcttcgc tccagcacca gcccggctca attcactggc
     8041 ccacacatcc ggcatattaa cagtaaaact agtaccctca ttaatagtgc aaccttgcgg
     8101 aacctgaata tccaggttac tgatccaggc attaaataat ggtactggcc catgttccgt
     8161 ttgccgctga ctggcataca atgaaactaa aagtacatta ttcagagaga catcagagat
     8221 gattttttta tccaacctaa ttttaatata gccttctgtt cctgaagacc agtaatctgc
     8281 tttacttctg tcagcaggat aattagtact atagttctcg aatggtactg ttttatctgt
     8341 catcgtccca ttttgattgt agagttttat atgtgttgaa atagatattt catgcgtaac
     8401 aggaataaat atttctgctc cctgtggatt acctgaagca cctgaaggat ctattcctga
     8461 aatacctgaa aaataaactt cataatcatc aagagaatta cagtatgctt tatatcgacc
     8521 tattttccag ttttgagatg gtaaaacagt accggctgaa ttgttgttaa catctgaaat
     8581 aataactgtg ccaaaatcat aaaaaaaggt cgctggcgta gtattgtttc ctggagtacc
     8641 aggatagcaa aactcacgat cccggcccat ttcagcatag gctacgacat ggtaccctaa
     8701 gaataaaaaa attataaaaa taattttcac tttatatccg gcataagtaa tcatacgatt
     8761 tccttttact aatccgtttt aaattgtgag cctgacacat ccatttgcaa cggattcata
     8821 tttactttcc atacaggcaa tacattatta taaatgtcca atgtctgcca tgttatttca
     8881 caaatccctt gcgctttaca ccatgattgt tgacgatggc taaatggtgc gatcatacca
     8941 ccatccataa ctttaccatc aacatgaagg ttatagagtg tcacatatct ggggcctgga
     9001 ttataaacgg taacaatttc attttttcgt gcccagcgta atttttgata tgaaatattc
     9061 gggtcttcag acaaatttga tggccgatag aatattttca tacgattgcg gaccgctatt
     9121 tgcaattgat tatcagtttt ggttggatga ctattttcca acgatggtaa tgcagcaaca
     9181 ttaagtataa ataaactttc ccggtcggca ggtaaactgc caccaacacg attaattctt
     9241 atttttgtat ttttgtcagg cttcaaaaca aacagtggcg gagttgcgat aaataccgtt
     9301 ccagaaaatg agggcaaaga ttcaaccgtt cccgtcaact gacgagcatc atcaggggag
     9361 actgcaacct tgactaacat atctctgtct gaagtgtttt tgagttcaac agatattgag
     9421 gactgatttt ccggatacgc aaaacgcgtt ccaccgatca ctaatcctgc ttgtgctgta
     9481 gagaataaaa gggaaaataa tactatgact accttaatca taggtgacaa taaacgatac
     9541 tgacgcattg gcatcccctg gttggacatt attttttaat gctttgtaag ccgcaaaaaa
     9601 atgcaatata ttatcaactg atgatgtaag tgaaaaacct atgcttttaa catcattact
     9661 aatactgcct ttgttcttat caaaaatcgt gagtgcaagc ccatctaccg agccatcttc
     9721 cagcttatat aaactggcat cttcagaaac tgtattgccg gtaaacttta tatacgcgtt
     9781 gccaacatca ctaccacagc gggttaacgt tatggaaaaa taaacctttt cagtaacatc
     9841 ccctgcactc cttaccgaac tactgggtac gtctggcata ttaactgtaa aattttttga
     9901 ctcatcagaa ataatgcagg ttttttcggt aattagccct tcaatacgaa ccactccggc
     9961 atgagtgata tcatcagcaa aagaatttgt tgatattata aacaaaaata aactaatgaa
    10021 tatcttcata tctccctcac tgataatcga tatcaaaata caacagagca tttgcttgcc
    10081 cggcatgtac attttcctga gttgatttat agcgaagttt aaacgtcaaa ttagcatcac
    10141 cgttttcatt taacactaaa tcacgaaaac ctgattcacc attaatagca atcgccacat
    10201 cattgttatc caatatttct attcccagtc cagtcgcact atctgcaccg gcggagagac
    10261 gcagtaatgc gctattaatg gcatctgcat caccagtaaa ccgcacattg gcgctcacac
    10321 cagaactata aacagtactt tttgcagtcg ggcatccttg tacattaatc gtaaacgttt
    10381 gactcggaga aacatcacct acatttaaaa attcattagc atcataagtg cccattttta
    10441 ccgactgatc tttacttccg gtagtaatgt cacagctttt tgccaacaaa atcccagtaa
    10501 atgtaacaac aactgaatca ttggcctgcc ctgagagcgg aaaagcaaaa aaagcgaaaa
    10561 gaataaaaaa tacagaatat ttagagaata ttaaattcat tagcggcact ccagagtagc
    10621 atagctaaca ggattattca gttctgtttc agggagcttg taattagcct gacatttttg
    10681 ctgaacacca tctccccact ggatattaat aacaccttct aaaggcaatc ctgagagata
    10741 aagttgtcca ttatcgccga caatcccggt aatttgacta ctatttttaa tctgtgcgct
    10801 ggctgtagca ccgaatggga ctgatttatt acgataacga actgtgaaca aaccacgaat
    10861 accgatatga gcagcaaact ctgcacgaac taacgcacct tttcccggaa taacttcagt
    10921 ggaagtgata tccagttcag tatgcatatt cattgacata ggatctaatg aaatattatt
    10981 acgtctatat tctgttgcat aaggaacaac tgcatatcca cgccagtcag tggatattcc
    11041 tgtcccccgc tggacatcca cattattcgc tccaggcgct tttatcaaaa cattagtttc
    11101 acctaatggt tgactcaacg taagaccatt ttcatgtaca acaagagcac cactgatgcc
    11161 atagtttatc tgttgattac cttcattatc gtaactgtag gcggaattca aggaaccata
    11221 tgccccacga taattaacac caacagaacc actactgcta ttatcctgat tagcaaaacc
    11281 atgttgtatg ttataactga gattatgctg ctccaacaga gaaccagata cccccgtata
    11341 ttggtttacc tgtccgtggt tatcctgact ataaccatac gttgcagtca tggcattcga
    11401 tgatgagcgc ccatcattcg tggaagataa ccaattactt ataggtaatg agacatttag
    11461 cgagacaatt ttatccgcat ttgtccctgg tgatttactg taattccacg caacatttag
    11521 atagataccc ttaatactgg tgttataacc aacttggtat aaagaatctt ttttatcagt
    11581 attccagtat gtttgctgac tagccgataa atacatagac ccgtagttac cgaaaggttg
    11641 cgatacacta gcctgaaatt ttccacgttt gctataacgc aagttataaa aatcattcca
    11701 gttataaacg taatctttat catctaacgt ttttggatca acaacagtcc ctgacatttg
    11761 ttggtatgtc gtatcactta aagtgtaaaa gccctgggtt gaataacgat agccaataat
    11821 atggaatgct gttcctgtct gaactaacga tttggaataa agaaaacgat aagattgccc
    11881 ttgatgtttg ctcccatcca caagcgaact gttagcctga gtaacatcaa acgatgttgc
    11941 accaaataca cccagattca ggccaaggcc gagcgccaac gcccggtaat cctcagagaa
    12001 ttgtgcccca ccataagccg taatatccca gggtaaaccc caaattaatt ccccctgaaa
    12061 tacataagga cttttctggt tatagctatt gcttcgatat tggccaaccg tagtagaata
    12121 ttttgaatga ccttctcgtt gcaggatggg gacagcggca taaggtacgg tatagacagt
    12181 ttctgaatta tcagactctt tgatggtaac ttccagatct cccgcagatg atgtcgggtt
    12241 aagatcgcta atctcaaacg gtcccggcgg catataggtt tgataaatga cataaccatt
    12301 ctgtttgatt gttacctgag cgcgactttt cgcaattccg cgcaccgtgg gagcgaaacc
    12361 actttgacta tcgggcaaca tattttcgtc agattccagt tttatgccac gaatactaac
    12421 actgtcgaaa acatctgatg atgaccatgt atctcctagc gttaattcac ttctgaaagg
    12481 aataataacg cgctgcaacg tactactgat atgctccagt ttacctgctg aattactccc
    12541 acgactccag gttgaataat cgcgaaaacg ccatgcacca atattgatcc cagcgcgaag
    12601 atttaaataa tagttatttg aagagtaatc accacgatcg ttattaccag aaaagtcata
    12661 attcagcaaa aaagctgtaa taccgttatc ccactgttct ggtggaatat atccgcgggc
    12721 gtcacgtttt attatcgcct gcggaaaggt cagtttaagt ctctgtgcat ccagctccgg
    12781 catgtaatgc acatcaggaa accagatacg caaatcaagg caactatttt cagataaagc
    12841 caaagcctgc tctggcagcg cattcttgtt aattccaagg cttaataagt cagcaagtga
    12901 aaggcaggga atcagttgat tctcatcgtc tgctttaaaa gttacagtac gagagagtaa
    12961 aggggaatca ttgacccaaa cttcgacttc atagctacca ggaagataag tcatcccttt
    13021 ctcgaaacgg gataagtcag caacagcttc agtatcaccc gacagaaaag cagggttaaa
    13081 gcgcattccc gcgattgaac atgcaggcat aataaccaga agaagcggta caacccgttt
    13141 catctgcttt tgtgccgcca tccaatattc gcatcttttg agctttaggt tcatatccct
    13201 gacctgtgtt gcatctgaac tatcccgttg ttactgcatt attcctttct gccgctccgt
    13261 cgtagcgcca aagtcattaa ttgtttgata agtaatttca ccagccaacg tcactgccga
    13321 cgagaccgta atatcgcttt tcggtggtac aaacgtattg ggcaatttac tcccttgtac
    13381 tttaattcct gtcagtgtca ggtaataagg cgaaggattg ataactttta gttgtttgcc
    13441 ctcatactta aaccgcaaca tatttcgtgc ttttcctggt tgcacaggta gattttccgg
    13501 gcgataaaat agcttgattt tgttttgtaa tgccaactgt aaggtattct tctgtgtatt
    13561 tttctcatct gttggcggaa ttgctttaac attcatccaa tacaaagttt cacgatcggt
    13621 cggcaaatta gttttaccaa gaaacatgat acgtaatgaa gcttctttac gcgttggcaa
    13681 cataaagagg ggaggagtga taacaaaatc ctggctcttt ttttccgttt tactgtcatc
    13741 aacccatgat tgaattaaat aactggtgtt gtcaccgcta ttttgcacac tgagtgttat
    13801 ttgcttactt tctgccggat aaatcaatcg cgttacgcca aggccaaacc cgcccgcgga
    13861 cgcattatta ataaataata tcgatagtaa aaaaagaatc cctgagcgta taatattttg
    13921 tgatactttt aattcatccc tgaatccata aacccatttt ttatttccaa taatacattt
    13981 catacagcat ccttgttgat gaaataacac ctgttattca tactctatta aataatttca
    14041 atctcaggtt acaacttttt gtaacctgaa atcgtattta tatttactcg tacagcacca
    14101 caaaacttgc tgttgactta actacaccag gtgcaacagt gctaccgttt tgagcataac
    14161 ctgcagtaaa ggccgcgctg aaatcatggg tcgtagcatt tcctggaatt gtataagcgg
    14221 cggtggaggc gctaccctct gccgcagtag tattcaggtt aatcagacca ttattcgtcg
    14281 tacctttgga gtaaattcga atccctacac cagtcgcccc cgcattgccg ctgttgtcag
    14341 catccgttga tccttctact tcgttagcga gcgttttatt agcgctatct gcggtaccgg
    14401 taaagcggaa ctcaattttc tgatcctctg tattgctaca ttcggtcaga ttaatattga
    14461 aatcggtctg gttgctcgcc ccctgtgctt ctatgtcgat accagtgaaa aggttggaag
    14521 gcacaaccgg caggacaact tcaatctgtt ttgaatcggt tgcgacctta caggtagctt
    14581 tcactacacg gccagtaaag cggattttcc cccccattga atcaacgttg tcatccatat
    14641 cagtgagcct gggataatca acggtgctcc agtcagtatc cgctccacca aattgtgctg
    14701 catcgccggg tgctgcggtt accgcagtag acatcagcat acctgatgcc agtaatactg
    14761 cggataaatt agataaattg aatttcattt atacctttcc ttttgttaaa aaatccaagc
    14821 aacattggtg tttttacatt ctgcgttaat aagttatatc tcacaaaaaa caagctgacg
    14881 ctggctaatt ccagaaggtt ctttagaaga gtaattgagt gaataatatt atatatgctg
    14941 cttagtaatg tacctaaaat aatccaaagc cgcaataagt gagtttatac tcacatataa
    15001 cacactgata atgataaaat ttatttttac gtggagttgt aagaggggta ataaaacata
    15061 aatcacatga ataatattaa tgcattcata ataaattaat attatttgaa ataacaaaac
    15121 accaaaaaca ttatcatcaa tcgttattta atatatatta atggtatata ttaaatagaa
    15181 taacatacat caaagttatt gtttattgtg tatagaaata ccgtgagttc cactctgtat
    15241 tacttcagcg gaacacacgg tatttagcca tatttgaact tacaatctca ccggatcaat
    15301 atgccagata tgatcggcgt actctttgat cgtacggtca gaagagaagt agcccatatt
    15361 ggcaatgttc agcatcgctt ttgcggtcca ctcttcctga cgctcgtaga gttcgtcgac
    15421 tttatcctga caatcgacat agctgcgata atccgccagt acctggtagt gatcgccgaa
    15481 gttgatcagc gaatcgacca gatcgcgata gcgacccgga tcttccggac tgaatacacc
    15541 gctgccgatt tgcgtcagca cctgatgcag ctcctcatct ttctcgtagt attcacgcgg
    15601 tttgtagccc tgacgacgca gttcttccac ttcttccgct gtgttaccaa agataaagat
    15661 attgtcagca ccgacatgat ccagcatctc gacattcgca ccgtccagcg taccgatagt
    15721 cagcgcgccg ttaagcgcaa acttcatgtt actggtgccg gaagcttccg tccccgccag
    15781 cgaaatctgt tcagacagat cggccgccgg aatgatcaac tgcgccaggc taacactgta
    15841 gttcgggatg aacacgactt tcagcttgtc gccaatctgc ggatcgttgt tgatcacttt
    15901 cgctacgtca ttgatcaaat gaataatgtg cttcgccatg taataggccg aagccgcctt
    15961 accaccaaaa atattcacgc gcggtaccca cttcgcatcc gggtcggcct tgatgcggtt
    16021 atagcgggtg atcacgtgca acacattcat caattgacgt ttgtattcgt gaatacgttt
    16081 gatttgcaca tcgaacagcg ctttcggatt taccaccaca ttcagctgct gggcgatata
    16141 ctccgccaga cgctttttgt tctccagctt cgcctgatgc accgcgtgat taaccattgg
    16201 gaaatcacag tgttgttgca gatcattaag caggctaagg tcggtgcgcc agttgcggcc
    16261 caggtgttcg tccaacacgg ctgaaagcga tgggttcgct accgccagcc agcgacgcgg
    16321 cgtcacaccg ttggtgacgt tggtgaaacg acccgggaag attttcgcaa agtcggcaaa
    16381 tagcgattgc accatcaggt tagagtgcag ctccgataca ccgttaactt tgtggctcac
    16441 cacaaccgcc agccaggcca tacgcacacg acgaccgttg gattcatcaa tgatcgacgc
    16501 ccgtcccagc agatcggtat cgttcggata ctgttcctgc aaggttttca ggaaatagtc
    16561 gttgatttca aagatgatct gcaggtgacg cggcagaatt ttacccagca tatcgactgg
    16621 ccaggtttcc agcgcctcgc tcattagcgt gtggttggtg taggagaaga cctgacaaca
    16681 cacttcaaac gcgtcgtccc agctaaattg gtgctcatcg atcagcagac gcatcatctc
    16741 aggaatcgac agcaccggat gagtatcgtt gaggtgaatc gcaattttat ccgccaggtt
    16801 atcgtaggtt ttatgcaact gataatggcg gcttaaaatg tcctgaatgg tcgaggaaac
    16861 caggaagtat tcctgacgca gtcgcagctc acgcccggag taggtggagt cgtccggata
    16921 cagtacgcga gatacgttct cggagtggtt tttatcttcc actgccgcga agtagtcgcc
    16981 ctggttgaat ttaccgaggt taatttcgct actggcttgg gcactccaca aacgcaacgt
    17041 gttggtcgcg tcggtgtcgt aacctgggat tatctgatcg taagctactc ccagaatctc
    17101 ttcggtttca atccagcgcg tttttttacc ttcctgctga atgcgaccgc caaaacggac
    17161 tttatagcgc gtgttgtggc gtttgaattc ccacgggtta ccgtattcca gccagtagtc
    17221 aggcgactct ttctggctac cgttaacgat gttctgcttg aacataccgt agtcatagcg
    17281 gatgccgtaa ccgcgccccg gcaaccctaa cgtcgccaga gaatcgagga agcaagccgc
    17341 cagacggccc aggccaccgt taccgaggcc cgggtcattt tcttcatcaa tcagctcttc
    17401 gagatttaat cccatcgctt ccagtgcgcc ctgtacatct tcgtaaattc ctagcgacaa
    17461 catggcgttg gaaagcgtac ggccaatcaa aaactccatc gacaggtagt aaacctgacg
    17521 agtttcttgc gacaactggg cacggtttga gcgtaaccag cgctccacga gacgatcgcg
    17581 cacagcaaat aacgttgcgt tcagccattc atgtttattg gcgacgaccg gatccttgcc
    17641 aatcgtaaac atcagcttgt aagcgataga gtgcttaaga gcttctacgc taagcgtggg
    17701 cgatgaatat gtaaacggag cattcatata ggcgtttcct gaaaactatt tcaagcgata
    17761 gtaaagctca cggtacgact tcgccgcgac ctgccagcta aaatccattg ccatagcctg
    17821 acgttgcaca aaccgccaca gtgaaggacg ggaccacagt acaaaagcac gtcgaatagc
    17881 ccgtaacagc gaccaggcat tactatcttc aaagacaaac ccactggcga cgccatctgc
    17941 aaggttctca agagaacagt cagaaaccgt atcagcaagc ccaccggtgc gccgcactaa
    18001 cggcagcgta ccgtacttca atccataaag ttgcgttaag ccgcacggtt caaaacggct
    18061 gggcaccaga atgacgtccg cgccgcccat aatgcgatgc gaaaatgctt cgtgatagcc
    18121 aatctgaacg cccacctggc cggggtattc cgctgccgcc gcaaggaaac cttcctgcag
    18181 caccggatcg cccgcgccga gtagcgccag ctgcccgccc tgctccagaa gaccaggtaa
    18241 ggcttccagc accaggtcga gacctttctg gctagtcaga cggctcacca ctgcaaaaag
    18301 cggcacttta tcgtcaacct taagccccat tgcgatttgt aactggcgct tattttccgc
    18361 tttatcttcc aacgtatcgc gggtgtaacg cgaggccaac agtaagtccg tctctggact
    18421 ccagattttc tcgtccacgc cgttcagtac gccggaaaga cgcccttcac ggtgacgctg
    18481 ttgcaacaga ccttccatac cgtaggcaaa ctgcggttcg gtgatctcgc gagcgtaggt
    18541 tgggctgact gccgtaatgt gatcggcata gtacagaccg gccttcagaa aagagatttg
    18601 tccgttgaat tccagcccat gaatattaaa gaatgaccat ggcaattgga tgtcattcat
    18661 gtgatgtgca taaaacatgc cttgataggc caggttgtgc acagtaaaca ccgacttcgc
    18721 cggacgcccg cgcgccgcca gatacgcagg cgcaaggcct gcatgccagt cgtgcgcatg
    18781 caccacatca ggacgccaga atgggtcaag cccgctggcc atttctgccc caacccaccc
    18841 cagcagcgca aaacgcaata cgttgtcggt ataggcaaat aagttggtat cgtgatacgg
    18901 gcttcccgga cgatcataga gatgcggcgc gtcaatcagg tatatgccaa ccccgttgta
    18961 atgaccgaac aacagcgtga tatgtccggc gaaggtatca cgacgggata ctacctgcgc
    19021 atcggtcacg ccacggcgaa tatcgggaaa tgcaggcaac agtacgcgag cgtcaacgcc
    19081 gtctgcgatt tgtgctgcgg gtaatgcccc aataacatca gccagaccgc cggttttaag
    19141 cagcgggaac atctctgaac atacatgtaa aacctgcatt atcgctcctg tttatgccct
    19201 aacttccgta gcatttcgcg cgttaccagc acgatgcctt cttctgaacg atagaaacga
    19261 cgtgcatctt cctctgcgtt ttcaccaatc accatgcctt ccggaataac acaagcacga
    19321 tcgatgacgc agcggcgcag acggcacgag cgacctaccc atacttccgg taacaatacg
    19381 gcggaatcaa tgttgcagaa tgaattcacg cgaacgcgcg agaacagaac ggactgcacc
    19441 accaccgaac cggagatcac acaaccgccg gataccagtg agttaagggt catcccatgg
    19501 ctaccggagc gatcctgcac gaatttcgct ggcggtaatg attcattgta ggtgcgaatt
    19561 ggccaattgc gatcgtacat atccagttcc ggcaccacag aggccagatc gaggttcgct
    19621 ttccagtaag cttccagcgt acccacatcg cgccagtacg gctcggcatc cgggtcggat
    19681 tgtacgcaag agagcgggaa cgggtgcgca taggccagac cggcttcggt gatcttggga
    19741 atcaaatctt tgccaaagtc gtggctggag ttctcatcgc gatcgtcttc ttccagcagt
    19801 tcatacagat agtcggcgtc aaagacgtag atacccatac tcgccagaga tttgctcgga
    19861 tcgttcggca ttgacggtgg gttagcaggt ttttcaacga attcgataat tttatcgttc
    19921 tcatcaaccg ccataacgcc aaatgcggag gcttcttcaa tcggtactgg catacaagca
    19981 acggtgcaac gtgcgccttt ttcgacgtgg tcgataagca tacgcgagta gtcttgcttg
    20041 tagatatggt cgcccgccag gatcaccacg tattccgctt tataacggcg gataatatcg
    20101 aggttttggg taactgcatc tgcggtgccg cgataccagt tttccccttt cattctctgc
    20161 tgtgctggca gcagatcgac aaactcgttc atttcttcat tgaagaatga ccagccgcgc
    20221 tgaatgtgct gcaccagagt gtgggactgg tactgggtga tcacgcccat acgacggatc
    20281 ccggagttga tgcagttaga cagcgcaaag tcgataatgc ggaacttacc gccgaagtgt
    20341 acggccggtt ttgctcgctt attggttaaa tccttcaggc gggtaccacg tcctcccgcc
    20401 agtatcaggg caacagattt caatggcagc tggcgcgcca acattaagtg atcgttcttc
    20461 tctaaactaa ccatgactaa ctcctttttt atcatctctg gaacacacac aatccgtgtg
    20521 caggtccctg ccagacagcc gtaatcactg ggttatcctc tccagcgaat gggggaatgg
    20581 cgtgccactc cccagcaggt aaaacaatct ctgttacctc aagcgtggcg ttaattgcga
    20641 tcaaaaagcg atccgagagc agaatttgca gctgtttcgg cccgttttgc cactcatccg
    20701 tgcttaaagg ttgagcatat cgatttagcc aacggacatt gccgtcgcct tcttcccacc
    20761 agcgattctc caccaaagcg ggaatgcgct tgcgcagatg gattaacgcg gcggtaaatg
    20821 cggttaaacc actgcttgcc tgcgaccagt ccaaccaggt taattggtta tcctgacagt
    20881 aggcattgtt attgccatgc tggctgtgac catgttcgtc accggccagt aacatcggcg
    20941 taccctggga gagcaacaac gttgttaaca gggcgtgaat gctgtcgcga cgccgttcaa
    21001 ccaggtcaag agtaccgcct aacccttctt taccatgatt gttactgtaa ttgttgttgg
    21061 tcccgtcgcg attttcttct ccgtttgctt cattgtgttt atggttgaag caaacgcagt
    21121 cgcgaagcgt aaaaccgtca tgcgcggtga cgagattaat cgcggcactc ggcagacgac
    21181 cattacgttt aaaaacatcg ctggaggcag caaaacgccc ggcaaacgcc cccagaggca
    21241 aatcataatg aagccagaaa cgacgggcag catcgcggaa atgatcgttc cactcggcaa
    21301 acagcggcgg gaaatttccc acctgataac caccaggagc aatatcccac ggttcagcaa
    21361 ttaacttcac ctgcgagagc accgggcagt tctggatagc ggtaaacaac ggcgcatcct
    21421 gacggaactc tggcgtacgg cccatgactg ccgccagatc aaagcggaaa ccatcgacgt
    21481 ggcaggtttc tacccaataa cgcaggcagg cgctggcata atccaccacc gccggatgac
    21541 tcaaattgag cgtgttgcca caaccggtcc agttgtgata atcgccgtct tctcttatcc
    21601 aataatagct acggttgtcg atcccacgca gagagaataa cgggccgtcg aggtccagtt
    21661 ccgcactatg gttgagcacg atatcaagaa tgacttcgat acccgcttta tgcagtgctt
    21721 tgattgcatc gcgaaactca tccagcgccg tttctgggga gcaggcatac gccggatgca
    21781 gcgcaaacat cgccaccggg ttataacccc agtagttact tagacccata cgttgcaggc
    21841 gtggttcgct ggcaaactgc gccaccggca gcagttccag cgcggtaatg cccaactgtt
    21901 tcaaatagtt gatcatcacc ggatgcccca gggctttata agtgccacgg atctcgaccg
    21961 ggatctccgg gtgcaggtac gttaatcctt tgacatgggc ttcataaatg atggtactgc
    22021 cccacggcgt gcgcggcgga gcatcatcct cccagtcata gtgatcaacc accactacgc
    22081 atttcggcgc aatggcggcg ttgtcgcgat agtcaggttc attatgaccg gcgtgcagca
    22141 gcgggttatc tttaaattcc ccgtcaattt gccgcgcgca aggatcaatc aacaacttcg
    22201 ccgggttaaa gcgatgcccc tcggcgggtt gccaggggcc atgaacgcga taaccataac
    22261 gcaaacccgg acgcgcatcc ggcagataac cgtgccaaat gtcgccactg tgccctggca
    22321 agtcatagcg atgttcctgg ccattggcgt caaagacaca cagttctacc cgctcggcat
    22381 gagcggagaa aagtgtgaag ttgacgccct gaccgtcgta atgcgcgccg aggggtgcgg
    22441 gtttgccaat ggcgagttgt gtcattctgc ctcccgaacc agccagatag tggccagcgg
    22501 tggtagtgtc aggcttaatg aatgctgacg accgtggctg gcaatctcat cgctgtgtac
    22561 cgtgccgcca ttgccgacat tactgccgtg atagtgcatg gaatcggtat tgaggatttc
    22621 acgccatttg cccggctggt taatgccgaa gcgataatca tgacgcggca ccggagtaaa
    22681 gttactggca acgatgattt cgttaccctc tttatcgcga cgcacaaaga tcagcaccga
    22741 gcgttctttg tcatccacca ccagccattc aaaaccgtac gggtcaaaat ccagttcatg
    22801 catcgcttta tggtggcggt aggtgaggtt cagatcgcgc accagacgct ggacaccgtg
    22861 gtgccagtta tcgccgcctt ccaacagatg ccagtcgagg ctggcgtcat ggttccactc
    22921 gcggccctgg gcaaattcgt tccccatgaa cagcagtttc ttgcccggga atgcccacat
    22981 ccagccgtag taggcgcgca ggttagcgaa tttctgccat gcgtcgcccg gcatccggtc
    23041 gagaatcgat tttttaccgt ggaccacttc atcatgcgac aacggcagga cgaagttttc
    23101 agtgtagttg tagagcatcc cgaaggtcag tttatcgtga tgatactgac gataaaccgg
    23161 gtcgagcttc atgtagtcca gggtgtcatg catccagccg aggttccact tgtaccagaa
    23221 gcccagaccg cccatatcct gcggacgaga aacgccaggg aaatcggtag actcctcagc
    23281 cattgtcacc gcaccggaaa cctgctcacc aagaatacgg ttggtattac gcaagaattc
    23341 aatcgcttca agattctcgc gcccgccaaa ttcgttcggg atccactccc cctctttacg
    23401 gctgtagtcg cgataaatca ttgacgctac cgcatcgacg cgcagcgcat caataccaaa
    23461 acgttcaatc cagtaaagcg cgttaccgac aaggaagtta ctgacttcac ggcgaccata
    23521 gttgtagatc agcgtgttcc agtcctgatg atagccttcg cgcggatcgc tgtgttcata
    23581 caagttcgtg ccatcaaatt cggcaagcgc aaagtcatcg gtcgggaagt ggcctggcac
    23641 ccagtcgaga atcacgttca gaccagctgc gtgtgcggca tcaatgaaat aacggaagtc
    23701 gtcgcgagta ccaaaacggc gggtcggtgc atacagaccg gttggctgat aaccccaact
    23761 gccatcgaag ggatgctcgt taatgggcag tagttcgagg tgggtaaagc ccatccattt
    23821 agcataaggc accagttgat cggccagctc gcggtagctc aaccagaaat tgttgtcggt
    23881 gtggcgacgc caggagccca gatgaacttc ataaatagag attggcgcat caaactgatt
    23941 cgcttttttg cgctcttcag tccgtacaac cttttccggc agcccgcaaa taagagacgc
    24001 ggtttccggg cgcatttgcg cttcgaaggc ataagggtcg gacttcagac gcaagttgcc
    24061 attggcatca atcatctcgt atttatagag ctgaccgtta tgcgccccag ggataaacag
    24121 ttcccagatg ccgctctctt tacgcaggcg catcgggtga cggcgaccgt cccagtagtt
    24181 gaattgccca accaccgaga cccgacgggc gtttggagcc cagacagaga aacgcgtacc
    24241 tgtgacgcca tccatagtat ctgcatgcgc gcctaaggtt tcatacgggc gcaggtgagt
    24301 accttcagat aatagccagg catccatttc ctggattagc ggaccaaaac ggtaaggatc
    24361 atcaatcagg ttttgctgac catgccagac aacagccaac tgatagcgga aaaaattctt
    24421 acgtcgcgga atgacgccgc taaagaatcc ccgtgagtcg agacactcca gttttgcgag
    24481 tttgcgcccg gttttcggtt caatcaccca cacatcggta gcgtcgggta aaagggcacg
    24541 gacttccagt cccgcggtgg ttttatgcat tcccagtacg gaaaaaggat ccgcaaaatg
    24601 gcctgcaatt agcgcgttaa tcacgtctct atcgatacga tcggacatgc ttgtcttcct
    24661 gttttattgt gtcaccccat ccaattggat ttttgacttc tggttgtgac atttttttga
    24721 cctgaacggc gcagcactct gtgcatcctc tctgcgtcgt cctcacttca ggtaaggctg
    24781 tgaatactca tgtattcagc cacccttaaa gaatagccaa tgctctattt aactcccggt
    24841 aaatcatgaa acatctgcgc ttactcctgt attacgcact aacaggggcg gcatcgcgcc
    24901 ccagatttaa tgaataaaga ttacgccagt tgacgaagca tccgacgcaa cggctccgcg
    24961 gccccccaca gcagctggtc acccacggta aaggctgaca ggaactccgg tcccatattc
    25021 agcttacgca ggcggcctac cggcgtggtc agcgtgccgg taacggcagc tggggttagc
    25081 tcacgcatag tgatttcccg atcgttcgga acgactttcg cccacggatt gtgtgcagcc
    25141 agcagttctt ccacggtcgg aatagacaca tcttttttca atttaatagt gaatgcctgg
    25201 ctgtggcagc gcaatgcccc gacacgcaca cataaaccat ctaccggaat tacggaagat
    25261 gtgttgagga tcttgttggt ttccgcctgc cctttccact cttcgcggct ctgaccgtta
    25321 tcgagctgtt tgtcgatcca cggaatcagg ctacccgcca gcggcacgcc aaagttatcc
    25381 accggcagct caccgctacg ggttaaggtt gtgactttgc gttcgatatc gagaatagca
    25441 gaggacgggg tcgcgagttc atctgccaca tggccataca gatggcccat ctgggttaat
    25501 aactcacgca tatgtcgcgc accaccgccg gaagcggcct ggtaggttgc aacggacacc
    25561 caatcaacaa gatcattggc gaataaacca cccaacgaca tcaacatcag gcttacggta
    25621 cagttaccgc caacaaaagt cctgatgcca ttatttaatc catcggtaat gacgtcctga
    25681 ttgacggggt caagaatgat gatggcatca tctttcatgc gcagagacga tgctgcgtca
    25741 atccagtaac cttgccatcc gctttcacga agctttggat agatttcgtt ggtataatcg
    25801 ccgccctgac aggtcacaat gatatcgagg gcctttagcg cctccagatc aaaggcatcc
    25861 tgaagtgtgc cagtggttcc gccaaaagac ggcgcagcct gaccaagctg agaagtagaa
    25921 aagaagacag ggcgaatggc gtcgaagtcg cgctcttcaa ccatgcgttg catgagaacg
    25981 gagccgacca taccgcgcca gccgataaaa ccaacatttt tcataagcgt tttttcctgc
    26041 aaagatgtgt gctgtataaa tgtgccggtc tcctcttggc acatctttca ccatacaaaa
    26101 agcagccaaa gtcgcaagtg aaattaatca atgatagcga agccatcagt aatgcgactt
    26161 atcctgcttt gttagcacgc agaaagtccg cggcaattat cagggaattt gagttatgaa
    26221 tgaaatcatt tctgcagcag ttttattgat cctgattatg gatccgctcg gaaacctacc
    26281 tattttcatg tccgtactga aacatactga accgaaaaga cggcgggcaa tcatggtgcg
    26341 agagttgctt attgctctcc tggtgatgct ggtgttcctg tttgcgggtg agaaaattct
    26401 ggcatttctc agcctgcgag cagaaaccgt ctccatttct ggcggcatca ttctgtttct
    26461 gatcgccatt aagatgattt tccccagcgc ttcaggaaat agcagcgggc ttccggcagg
    26521 tgaagagccg tttatcgtgc cgttggcaat tccgttagtc gccgggccga ctattctcgc
    26581 cacgctgatg ttgttgtctc atcagtaccc gaatcagatg ggacatctgg tgattgctct
    26641 gctgctggcc tggggcggca cctttgtcat cctgctacag tcttcgctat ttttacgtct
    26701 gctgggcgag aaaggggtga acgcacttga acgcctgatg ggattgattc tggtgatgat
    26761 ggcaacccag atgttcctcg acggcattcg aatgtggatg aaggggtaac gaggagagtt
    26821 tttcgaaaag agcccggtag cgtcagaact accgggtaaa caaacttaac tcaacagctg
    26881 gaacgcaatc atcccaacga tggcaccgac agtgccgagg atggtttcca tcatggtcca
    26941 ggttttcagc gtttcggctt cggtcgcgcc ggtaaattta ccgaacaacc agaaaccggc
    27001 gtcgttaacg tggctgacaa caatcgaacc accggcgata caaatcgaca gcgccgccat
    27061 ttgcgcacca gagtagttca gttgttcaat gactggcatc accagtccca ccgccgttaa
    27121 acaggctacg gtggcagacc cctgaatgat gcgcactgca gctgccagca cgaagcaggt
    27181 gatagcaatc ggcaggccca tgccggttaa cgcttcgccc agtgccggac ctacgccaga
    27241 gtcaaccagc acctgtttga acacgccgcc cgcaccaatc accagcagaa tgatccccgc
    27301 cggttgcagc gcgtgaccgc aaatctccat cactttgtct tttggcatgc cctgacgcat
    27361 tgccaggcca taaatcgcta ccagacaagc aaccagaatc gcggtaaacg gatgaccaat
    27421 aaactcgaac cattcgtaag cggtagagcc ttctggcaca aaacgcgcgg caatggtttt
    27481 cagccccacc agcaccagcg gcaacaggat cagcgacagg ctgaatccga aagatggcat
    27541 tttaccttcg ccgagatgcg gttcgctgat gtcgtcagga atatgcagct caacgtaacg
    27601 gctgatgaag ttaccccaca gcggcccggc aataatcatt cccggaattg ccgcacacag
    27661 gccaatcagg atcatccagc caaagtcggc gttcatctgc gatgccagca gcattggcgc
    27721 tggccccggc accaggaacg cagccgctgc cgccacgcct gcaaataatg ggattaccag
    27781 cttcaccagg ttcgtaccgg tatgacgcgc cattgagaaa gcaacgctaa tcagcagaac
    27841 aatcgccact tcaaagaaca gcggtagcgc acaaaccagc cccgcaaggc caatggcata
    27901 atgcgcgcgg ctgtgaccga aggatttaag cattttgacg gcaatctgat cgactgcgcc
    27961 ggtttcatgt aagatcttgc caaacatagc tcccagggcg acaaccaccg ccaggaagcc
    28021 gagggtgcct cccatccctt tttccatcgt cgctgcgatt ttatcgagcg gcataccgga
    28081 aaaaaggcca gcccccatgg acaccaccat taaagccagg aaagcgtgca tacgcgcctt
    28141 catgactaaa aacagcagca gtaaaacaga ccctactgct gttaaaacaa gcgttaatgt
    28201 agtcactact tatttgcctt ttttaataac ctcaatggtg cttgccacaa caccttccag
    28261 cggttgatcg atatccacca ccagtacatc ggtttcgtcc gcacccggct cctgcagcgt
    28321 ttcaaactgc gtcaccaaca tttgggtttt aaagaaatgg cctttgcgcg ctttcaggcg
    28381 gctttcaatc acatcaaaat cacctttcag gtagatgaaa gagagattcg gattaccttc
    28441 acgcagcaag tcgcgatagt gttttttcaa tgcagaacag acgatcagtg acactttatt
    28501 agtgcgctgc atagcaaacg cggcgtcgtt cagcgcctgc agccacggtt tgcgatcgtc
    28561 atcattcagc ggttcgccag acgccatttt ttcgatattg cgccgtggat ggaggaaatc
    28621 gccatcaaga aacgcggcat gaagttgatg cgccacttca cttgcgaccg cagatttgcc
    28681 gctgcccgat acgcccatca agacgtaaat gtggtgatca tggttagtcg tgctcaaagt
    28741 ggtgccccca caatacaaga attaaaaatg ttacgggtaa ctgttatcgg taacattgtc
    28801 cagccggaca atatcagaag caatatccat ccgtgcctta agtgtataag tgtgagctac
    28861 ttcaaatttg tgggcttaaa tagatccgcc cggtgacaag gtgaaaccta aatctaacat
    28921 tttcggtgtc acagattcgc cacgaatacg cgccagcagg cgttcagcgc caatactgcc
    28981 catccgctca cgcggcgtca ggacgctcgc caaccgtggc tccatcacct ggccaatgtc
    29041 atgaccgtgg aaaccggcaa tcgccatatc gtcaggaact ttcaacccca gacgctgaca
    29101 ttcaaacgcc gcgccgaccg ccaggtcatc attagtacag aacacgccat ccagctgcgg
    29161 atattcccgc cgtgcctggc gaatcagttc aataccggaa gaataagaag aagattgctc
    29221 aaccatcacg ctatacggca ccaggcctgc atccagcatc gcctgttcat atcccttctg
    29281 tttgatgata gtacgttcgt cgagacgtgc gccgagatag gcaatgtggc gatgcccgcg
    29341 agcaataatg gctgtggtca tctggcgtgc cgcttcaaag ttatcaaaac cgacggcgat
    29401 atcgaggcaa ggcgacttgc tgtccatcag ttccaccacc gggatccccg ccacttcaat
    29461 catctttaag gtgcgcggcg tgtgggtacg ttcggtgagg atcagtccgt cgatattcca
    29521 tgagagcatg gattcgagac gttcttgctc catttccggt ttataaccgt agtgggccag
    29581 catggtctga taaccgtgcg cgtcggtgac gctttcgatt ccgcgtaata cttccgcgaa
    29641 aacctggttg gtgagagaag gtaacaggac gccaatcgcc cggctggtgg cgttagagag
    29701 gatatcgggt gcgcgattgg gaatataacc cagttcatca agagcggcgg caatcttgcc
    29761 gcgtagagcg acggaaacct gctccgggtt acgcaaaaag cggctgaccg tcattttggt
    29821 cacgcctaca cggtcagcca catcctgaag tacgggtctt ttctttttca tcgtcctgaa
    29881 ggtacaaaag agatagattc tcttagttta acacggacgc atcagaacct tcccggggaa
    29941 aacagtgctt gtttacaatt attagatata cactaaatca attgcatcgc attgtgctaa
    30001 cgacgcggca aaaccccgga agcgtacatc agcacgcgac cggagcgagc gaggaagcct
    30061 gcaaaaaggc aggcttcaaa gatgccgttg agctttaaac cggcggcaga tcgaacagta
    30121 acacttcgct gtcgctatcc gcatggatgg agattgcctg ctcatcccag attgccagac
    30181 catcgctgat cgaggctttc acgccgttaa tggtgacatt gcctttcacc acctggatcc
    30241 agacgcggcg ttcagcggca atctgatgca ccgactgctc atctttcagc aacgcccagc
    30301 ggtacagttc catatcctga tgcactttca atgaaccatc acgcgcatct ggcgaaagca
    30361 ccagttgttt gccctgcact gcatcgaagc gacgctgttc ataacgcggc gtaatgccgt
    30421 tttcttccgg catgatccag atctgataca ggtgcagacg ctcggtgctg cttgggttgt
    30481 actctgagtg acgaataccc gtacccgcac tcataatctg gaactcgccc gccggaacct
    30541 gctctttatt gcccatgctg tcctggtgct caacagtacc ttccagcacg taggtcaaaa
    30601 tttccatatc tttatgcggg tgagtgccga agccctgccc tgcttcaatc acgtcgtcgt
    30661 taatcacgcg cagcgcggag aagcccataa agttcggatc gtaatagttg gcaaaagaga
    30721 aagtatgcca ggagtccagc cagccatgat ttgcatgacc acgttcattt gctttgcgta
    30781 agtagatcat ttgtattcgc cccctgaatg atttcgatgg gcttagtgtg gacccgatct
    30841 gcctgggatg atagagggtg aaaattgacc cctctgttca aaaaaattga actattcgag
    30901 gggccatcaa acttacttcg cgagagtcac tgtggaggga gaggcttgct caaatccgcg
    30961 ttcaaggatt tccagattgg taagaacttc agattccttg acgtaatttg gcgcaccgtt
    31021 ggtgatggtt tgatacaacg catcataaac gcgcccgtaa tcgcccatct ccggcttcat
    31081 ctcttctctg accgtcacac cctcgtcatt gacatactcc agcacaccga ccgaatcatc
    31141 cgctgcgaat cccggttcgc ccggcataat attagccttg aggctggttt cctgctggtc
    31201 gataccgtat ttaataaacg aacctttctt accgtgaacg ataaatttcg gataatcgat
    31261 tttcaccaga tggctggttt tgacgatggc tttcaagtcg ccataaaaca gttgcgcttc
    31321 aaaggtgtcg tcaggattgg ctttattgcg caggctgcgg atgtcataag cgacgtgatc
    31381 cgggcgaccg aacagagaaa taatctggtc catcgtatgc acaccaaggc catagaacgc
    31441 gccatcctgc ggcaacccag gtttggtttc tgccaccggg cggtaatagt caaaatggct
    31501 ttccacttca acaatctctc ccaacttgcc gctttcaatc gcttttttcg ccgtcaggaa
    31561 gcaggaatca aagcgacgat tctgatacgg cgtgacggtc agccctttgc ttttcgccag
    31621 cgcaaacagc tccttcgcct gggcaagcgt cggggtgaac ggtttttcga ccagcacatt
    31681 tttcccggct tccagcgcgc gtttcgcgta ttcgaaatgg ctgtccgcgt gggtgcagac
    31741 gacaaccagc ttaacatcgg gatcgtttag cacttcgtcg agatcgctgg tgaaatggat
    31801 atgagagtaa atgggagcct gttcttccgg ctttgcatgg cggcgaaaaa tatgcgcgac
    31861 atgccagcta tccttgcggt taagtacata cggcagatgg taacgggtgg tgcttttgcc
    31921 gaagccaata aaggcgcagt tgatgaccat gatttcgtcc tttttaaggt ggttatttac
    31981 accttagcgc aaagcggacg tggttcctac tggagtgcgc ataacgaaca caagcacttc
    32041 ccgaggataa attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa
    32101 tactggaagc aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc
    32161 gcgagacaat aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca
    32221 tttgactcgc attcgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa
    32281 ggaatgagtg agatagtgat acgccacgca gaaacacggg attacgaggc catcaggcag
    32341 attcacgccc agccggaggt gtattgcaac acactacagg tgcctcatcc ctccgatcat
    32401 atgtggcagg aacgactcgc cgatcgcccc ggcatcaagc aactcgtcgc ctgtattgat
    32461 ggaaccgtag taggtcatct caccattgat gtacagcaac gcccgcgccg cagtcatgtt
    32521 gccgattttg gtatctgtgt cgactcccgt tggaagaacc gcggcgtcgc cagcgccctg
    32581 atgcgagaga tgattgaaat gtgcgacaac tggttgcggg tagatcgtat tgagctaacg
    32641 gtgttcgtcg ataacgcgcc agcaattaag gtctataaaa aatttggctt tgagattgaa
    32701 gggactggca agaagtacgc attacgtaat ggtgaatatg tcgatgcgta ttatatggcg
    32761 cgggtgaagt aagatagtgc cctttttctg agatggaaaa agggtgtcat tcaaaatcgg
    32821 cataccttcc tttaaggtat ttattttccc aatacatata ttgagat
//
LOCUS       DAZGTJ010000054        32679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000054 DAZGTJ010000000
VERSION     DAZGTJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.054"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..574
                     /locus_tag="ACHK8E_003038"
     CDS             <1..574
                     /locus_tag="ACHK8E_003038"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0345274.1"
                     /translation="QSGGTPSRKETSFWDGGTIKWLSSGEVKGKIVLDTKEKITDTGL
                     LNSSSKLWEKYTTVVAMYGATAGEVCIIGDKMAANQACCGLYSKTFPFFVYNFVCNKA
                     NELASKATGSAQQNLNKLIISTTKFICPSNDIITIFEDNVTPLFMKWFSNSSENNTLT
                     ALRNTLLPKLISGELSVEDLPDHIKQTEAA"
     gene            669..3932
                     /locus_tag="ACHK8E_003039"
     CDS             669..3932
                     /locus_tag="ACHK8E_003039"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP0345275.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            4147..4485
                     /gene="symE"
                     /locus_tag="ACHK8E_003040"
     CDS             4147..4485
                     /gene="symE"
                     /locus_tag="ACHK8E_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP0345276.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4527..4580)
                     /locus_tag="ACHK8E_003041"
     CDS             complement(4527..4580)
                     /locus_tag="ACHK8E_003041"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP0345277.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4569..4724)
                     /locus_tag="ACHK8E_003042"
                     /pseudo
     CDS             complement(4569..4724)
                     /locus_tag="ACHK8E_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4867..6279
                     /gene="yjiR"
                     /locus_tag="ACHK8E_003043"
     CDS             4867..6279
                     /gene="yjiR"
                     /locus_tag="ACHK8E_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0345278.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6521..7465)
                     /locus_tag="ACHK8E_003044"
     CDS             complement(6521..7465)
                     /locus_tag="ACHK8E_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0345279.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7932..9164
                     /gene="mdtM"
                     /locus_tag="ACHK8E_003045"
                     /pseudo
     CDS             7932..9164
                     /gene="mdtM"
                     /locus_tag="ACHK8E_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            9205..10485
                     /gene="yjiN"
                     /locus_tag="ACHK8E_003046"
     CDS             9205..10485
                     /gene="yjiN"
                     /locus_tag="ACHK8E_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP0345280.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10601..11752
                     /gene="yjiM"
                     /locus_tag="ACHK8E_003047"
     CDS             10601..11752
                     /gene="yjiM"
                     /locus_tag="ACHK8E_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP0345281.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11762..12529
                     /gene="yjiL"
                     /locus_tag="ACHK8E_003048"
     CDS             11762..12529
                     /gene="yjiL"
                     /locus_tag="ACHK8E_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP0345282.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12526..12783
                     /gene="ytiA"
                     /locus_tag="ACHK8E_003049"
     CDS             12526..12783
                     /gene="ytiA"
                     /locus_tag="ACHK8E_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP0345283.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12848..13708
                     /gene="yjiK"
                     /locus_tag="ACHK8E_003050"
     CDS             12848..13708
                     /gene="yjiK"
                     /locus_tag="ACHK8E_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0345284.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13776..14954
                     /gene="yjiJ"
                     /locus_tag="ACHK8E_003051"
     CDS             13776..14954
                     /gene="yjiJ"
                     /locus_tag="ACHK8E_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345285.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14967..15521)
                     /gene="kptA"
                     /locus_tag="ACHK8E_003052"
     CDS             complement(14967..15521)
                     /gene="kptA"
                     /locus_tag="ACHK8E_003052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP0345286.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15771..16454
                     /gene="yjiH"
                     /locus_tag="ACHK8E_003053"
     CDS             15771..16454
                     /gene="yjiH"
                     /locus_tag="ACHK8E_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0345287.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16451..16912
                     /gene="yjiG"
                     /locus_tag="ACHK8E_003054"
     CDS             16451..16912
                     /gene="yjiG"
                     /locus_tag="ACHK8E_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP0345288.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16925..18097
                     /gene="iadA"
                     /locus_tag="ACHK8E_003055"
     CDS             16925..18097
                     /gene="iadA"
                     /locus_tag="ACHK8E_003055"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0345289.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            18162..19073
                     /gene="hypT"
                     /locus_tag="ACHK8E_003056"
                     /gene_synonym="qseD"
     CDS             18162..19073
                     /gene="hypT"
                     /locus_tag="ACHK8E_003056"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP0345290.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            19080..19451
                     /locus_tag="ACHK8E_003057"
                     /pseudo
     CDS             19080..19451
                     /locus_tag="ACHK8E_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19499..19882)
                     /gene="iraD"
                     /locus_tag="ACHK8E_003058"
     CDS             complement(19499..19882)
                     /gene="iraD"
                     /locus_tag="ACHK8E_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP0345291.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
     gene            complement(20296..21069)
                     /gene="uxuR"
                     /locus_tag="ACHK8E_003059"
     CDS             complement(20296..21069)
                     /gene="uxuR"
                     /locus_tag="ACHK8E_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP0345292.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(21284..22744)
                     /gene="uxuB"
                     /locus_tag="ACHK8E_003060"
     CDS             complement(21284..22744)
                     /gene="uxuB"
                     /locus_tag="ACHK8E_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP0345293.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQSADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(22825..24009)
                     /gene="uxuA"
                     /locus_tag="ACHK8E_003061"
     CDS             complement(22825..24009)
                     /gene="uxuA"
                     /locus_tag="ACHK8E_003061"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP0345294.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            24349..25692
                     /gene="gntP"
                     /locus_tag="ACHK8E_003062"
     CDS             24349..25692
                     /gene="gntP"
                     /locus_tag="ACHK8E_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP0345295.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(25865..26767)
                     /gene="fimH"
                     /locus_tag="ACHK8E_003063"
     CDS             complement(25865..26767)
                     /gene="fimH"
                     /locus_tag="ACHK8E_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0345296.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(26787..27290)
                     /gene="fimG"
                     /locus_tag="ACHK8E_003064"
     CDS             complement(26787..27290)
                     /gene="fimG"
                     /locus_tag="ACHK8E_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP0345297.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(27303..27833)
                     /gene="fimF"
                     /locus_tag="ACHK8E_003065"
     CDS             complement(27303..27833)
                     /gene="fimF"
                     /locus_tag="ACHK8E_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP0345298.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(27843..30479)
                     /gene="fimD"
                     /locus_tag="ACHK8E_003066"
     CDS             complement(27843..30479)
                     /gene="fimD"
                     /locus_tag="ACHK8E_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP0345299.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(30546..31271)
                     /gene="fimC"
                     /locus_tag="ACHK8E_003067"
     CDS             complement(30546..31271)
                     /gene="fimC"
                     /locus_tag="ACHK8E_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0345300.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(31308..31847)
                     /gene="fimI"
                     /locus_tag="ACHK8E_003068"
     CDS             complement(31308..31847)
                     /gene="fimI"
                     /locus_tag="ACHK8E_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345301.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(31912..32466)
                     /gene="fimA"
                     /locus_tag="ACHK8E_003069"
     CDS             complement(31912..32466)
                     /gene="fimA"
                     /locus_tag="ACHK8E_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0345302.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         7861 a         8315 c         8083 g         8420 t
ORIGIN      
        1 tcagagtggg ggaacgcctt caaggaagga aacttcattc tgggatggcg gtacaataaa
       61 atggctttca tctggcgaag tgaaaggaaa aatagtctta gatacaaaag aaaaaatcac
      121 agatactgga ttactaaata gttcatctaa attatgggaa aaatatacaa ccgttgttgc
      181 aatgtacggt gcgactgcgg gtgaagtgtg tataattggt gataaaatgg cggccaatca
      241 agcatgttgt ggattgtatt caaagacatt cccatttttt gtctataatt ttgtctgcaa
      301 taaagcaaat gaattagcat caaaagctac aggttcagcg caacagaacc tcaataaatt
      361 aattatatct acaacaaaat ttatatgccc ctcaaatgat ataattacta tctttgaaga
      421 taatgtaacc ccattattta tgaaatggtt ttctaattcg tctgaaaata ataccctaac
      481 agccctccgc aataccctgc tccccaaact catctccggc gagctatccg tggaggatct
      541 cccggatcac atcaaacaaa ccgaagccgc ataacgcatt ttgcccctgt aaaatcaggg
      601 gctttctggt aaggttttct actgatacgg gaatgcttac cagaaattag ccagggttgg
      661 agcacgatat gagtctctct ttcagtgaag caaaattaga acaagcgatc attgaactgt
      721 tacaggatca gggatatcaa cacctgatcg gcgataatgt cccacgttca agtctcgatc
      781 aggtcattat cgaagacgat ctccgtcatt atttagcggc acgctaccag cctgatggca
      841 ttactgaaga agagattcag cgactgatca aacagttcac cacgcttccg gcctccgatc
      901 tttatgaaag caacaaaaca ttttgtcgct ggctggcaaa tggttttctg ttcaaacgcg
      961 acgatcggca acaaaaagat ctctatattg aattgctcga cacccggcat ctacctgccg
     1021 cactgcgcca gatatttgac gccgaagatg tcccgttgca acaggctgcg gaactcccgc
     1081 cttcctatat taacccgccg cttaaccgga ttaagattgt taaccagctt aaaatctccg
     1141 gcaaagataa tcagactcgt attcctgacg gcattctcta tatcaacggt ctgccactgg
     1201 tcgtctttga atttaaaagc gcggtgcgcg agcaggaagc caatataggc gatgcctgga
     1261 agcagctctg taaacgctac cgccgggata ttccgcaact gtttatctac aacgcgctct
     1321 gcattattag cgatggcgta aataaccgga tgggcaacct gtttgcaccc tatgaatatt
     1381 tttactcatg gcgcaaagtc accggtaatg aaaaccgtga acaggacgga attccatcat
     1441 tgcactcaat gattcagggg ctgtttcatc cggtacgtct gctggatgtg attaaaaact
     1501 ttatctgctt cccggataaa gccaagcacg aagtaaaaat ttgctgccga tatccgcagt
     1561 actatgccgc ccgcaaactc tattacagca tcaagcaagc gcgtaaacct ttcggtagcg
     1621 gtaaaggcgg tacctacttt ggcgcaacgg gctgcggtaa aagctacacc atgcaatttt
     1681 taacccgctt gttaatgaaa agcgtggagt ttgccagccc gaccattgtt ttgatcaccg
     1741 accgcaccga tctggacgat cagctttctg cgcaaatgtg caacgccaaa aattacattg
     1801 gcgacgacac catcgttcct gttaccagcc gtgaagattt gcgtaatcaa ctggcgggac
     1861 gcaatagtgg cggtgtattc ctgacaacga tccataaatt caccgaagac accgaactcc
     1921 tttctgaacg cagcaatatc atttgcatct cggacgaagc acatcgcagc caggttaacc
     1981 tcgaccagaa agtcatcgtc gataaagaaa gcggaaaagt gcgcaaaacg tatggctttg
     2041 cgaaatacct gcacgattca ctgccaaatg ccacctatgt tggctttacc ggcacaccga
     2101 ttgacgcgac gctcgatgtc ttcggtgagg tgatcgacag ttacaccatg accgaatccg
     2161 ttcaggatga aatcactgta cgcatcgtgt acgaaggccg tgcggctaaa gtgatcctgg
     2221 actccagcaa actggaagaa gtcgaaaagt attacgaaga gtgcgcaaac gcaggcacca
     2281 atgagtggca aatcgacgaa agcaagaaag ccacggctac catgaatgcg gttctgggtg
     2341 atgaagatcg gttaaaagcc ctcgcggaag attttgctaa acattatgaa aaacgcgtag
     2401 ccgaaggttc caccgtaata ggcaaagcca tgtttgtttg tgccagccgt gaaattgcct
     2461 gggatttcta ccgccagctt aaagctattc gccctgcctg gtttgaagtg aagcaagcac
     2521 ctgatggcgt cgtcctgacg gaacaggaac aaaaagagtt accgccttct gaaatggtga
     2581 aaatggtcat gacgcgcggt aaagatgacg acgaggcgct ttatgattta ctgggcacga
     2641 aagaatatcg taaagagctg gataagcagt tcaaaaacgc taaatcgaat ttcaaaattg
     2701 ccattgtcgt tgatatgtgg ctgaccggtt ttgatgttcc tgaactggat accatctata
     2761 ttgataagcc cttacaaaaa cataacctta tccagactat ttctcgcgtt aaccgtaagc
     2821 tggaaggcaa aagcaaaggg ttagtggtgg actacatcgg cattaaaagt cagatgaacc
     2881 aggcactggc aatgtattcc cgcattgatg ccaccaactt tgaagatatt cagcaatcgg
     2941 taatagaagt taaaaaccat ctcgatttgt tggggcaagt cttttacgaa tttgacagtc
     3001 gggattattt tagtggtgag ccacaagcgc aattatcctg cctcaaccgc gcggcggaat
     3061 ttgttctgcg tacccagaaa gttgaacgtc gttttatggg actggttaaa cgcatgaaag
     3121 ccgcctacga cgtctgctgc ggcagcgaag cattatcaca gacagaacgt gatcatattc
     3181 actattatct tgctgttcgt tcaattgttt tcaaactgac gaaaggtgac gcaccggatg
     3241 tcacccagat gaatgcacgc gttcgtgaaa tgattgcaga agcgctaaaa gctgatggcg
     3301 tagaagaaat ttattttctt ggcgataaaa aagcggaatc catcgatatt tttgacgaag
     3361 attatctggc gcgaattaac aagatcaaac ttccggcaac aaagatccag ttattacaaa
     3421 aattactgga aaaagcgatc agcgacttca agaaagtgaa ccagttgcaa gggattaact
     3481 tcacccgccg cttccaggcg attatagatc gttataatga gcggcgagaa gatgatgtac
     3541 tcaacggtga agaattcgat acattcagtc aggaaatgac cgatattatc tatgatatta
     3601 aaaccgaaat gggcacctgg gccgatttag gcattgatat tgaagaaaaa gcctttttcg
     3661 acattcttgc tcatatgcgc gataaatatc agttcaccta tgacgatgaa aaaatgctgt
     3721 cactggcaaa agagatgaaa agcgtggttg acaacacatc gaaatatcct gactggagta
     3781 aacgcgatga tattaaagcg aaactgaaag ttgaacttat tctgcttcta cacaagcata
     3841 agttcccgcc agtagcgaat gatgatgttt atatgggtgt gctggcacag gcagagaatt
     3901 ttaaagaaca tcatatgagt gctttaaatt aattatgaag gccggataca ctttgttatc
     3961 cggccattct gaattaacgt taatttctct tccagatctt tccagtaacg cttttattat
     4021 tttctatttt tagctggact ttccctgtat ttactgatga tatatccagg tatttagcgc
     4081 ggtgcgaatg tgcgccaaca cacccgcacc gctcatcaca atccctattt cacgagaata
     4141 gtaatcatga ctgacacgca ttctattgca caaccgttcg aagcagaagt ctccccggca
     4201 aataaccgtc aattaaccgt gagttatgcg agtcgctacc cggattacag ccgtattccc
     4261 gccatcaccc tgaaaggtca gtggctggaa accgccggtt ttgccaccgg cacggcggta
     4321 gatgtcaaag tgatagaagg ctgtattgtc ctcaccgccc aaccacccgc cgccgaagag
     4381 agcgaactga tgcagtcact gcgccaggtg tgcaagctgt cggcgcgtaa acaaaggcag
     4441 gtgcaggatt ttattggggt gattagtagc aaaacgccac gctaagtttc tctccccttc
     4501 cccatacggg aagggaaatt gccccctcac tccacatcct ccctgcgtaa cccgatatcc
     4561 ctcaaccgtt catcactcat ctgctgtaca cccgccgggc ctgcatttgc cgccgccaac
     4621 gcctcaccgc ctgtcagagt tgcacaagtc tgataaacgg cgttttggct ttgttttcgt
     4681 gaaattccat ctgctgcctc ctcatctttc agaggcttca tcatccgtct tctggcagtt
     4741 cacaatacag atgcaaatat tagttttatt taacatacag acaggttaaa acagcatctg
     4801 catccggttt tttagcctca tctgtaccgg tttttctctc tgtatggtcc aaataaggaa
     4861 tacagcatga cgcgttatca acatctggcg actctacttg ccgagcggat tgagcaaggg
     4921 ctgtatcgtc acggggagaa attgccgtcg gtgcgcagct taagtcagga gcacggcgtc
     4981 agtattagca ccgtgcagca ggcgtaccag acgctggaga cgatgaagct catcactccg
     5041 cagccgcgtt cgggttattt tgtcgcacaa cgtaaagccc agccgccagt accgccgatg
     5101 acgcgtccgg tgcagcgccc ggtggaaatt acccagtggg atcaagtgct ggatatgctg
     5161 gaggcgcata gcgacagttc cattgttccg ttaagtaaaa gcacgccgga tgtcgaaacg
     5221 cccagcctga aaccgctgtg gcgggagcta agccgggtgg tgcagcataa tctgcaaacc
     5281 gttctcggtt atgacttgtt agccggtcag cgagtgttgc gagagcagat tgcccgcctg
     5341 atgctcgaca gcggctcagt ggtcaccgct gatgacatca ttatcaccag cggctgccat
     5401 aactcgatgt cgctggcgtt aatggcggta tgtaaaccgg gcgatattgt cgcagtcgaa
     5461 tccccctgtt attacggttc aatgcagatg ctgcgcggta tgggcgtgaa agtgattgaa
     5521 atcccaaccg atccagaaac tggcatcagc gttgaagcgc tggaactggc gctggaacag
     5581 tggccgatta aaggcatcat tctggtgcca aactgtaata atccgctggg atttattatg
     5641 ccggacgcac gcaaacgggc cgttctctct ctcgctcagc gtcatgatat tgtgattttt
     5701 gaagatgatg tctacggcga actggcgacg gagtatccgc gcccgcggac cattcattcc
     5761 tgggatatcg acgggcgagt gctgctgtgc agctcgttca gtaaaagtat tgcaccaggc
     5821 ctgcgcgtgg gttgggtcgc accggggcgt tatcacgata aactgctgca tatgaaatat
     5881 gccatcagca gctttaatgt accgtccacg caaatggcgg cggcaacgtt tgttctggaa
     5941 ggtcactatc atcgccatat ccggcggatg cggcagatct atcagcgcaa tttggcgctt
     6001 tatacctgct ggatacggga atattttccc tgcgaaatct gtattacgcg cccgaaaggc
     6061 ggatttttac tgtggataga attgcctgaa caggtcgata tggtttgcgt tgcgcggcag
     6121 ctgtgccgca tgaaaatcca ggtggcggca ggctcgattt tctcagcttc cggcaaatac
     6181 cgtaattgtc tgcgcatcaa ctgcgctttg ccgctcagcg aaacctatcg cgaagcactg
     6241 aagcaaattg gcgaggccgt gtatcaggca atggaatgaa ttcgaattag tggcgaggcc
     6301 cctgatttcc tctcacgaat cgcctgtgac tcgttttatc tttgcagggg catcgccacg
     6361 ctccagcatg gcttaggcaa agtgtgcttt gttcataccg gatgcggcgt gaacgcctta
     6421 tccggcttac aaatcgcgca aattcaatag attgcagagg taatgtaggt ctgataagcg
     6481 tagcacatca ggcagctttg cgtttgtcat cagtctctga ttagtactcg atccatggat
     6541 cgcgcttgct ttcaggcaag ggttgctcta atgcctgcat ttgctctgcc gaaagtccag
     6601 tatatcgttg tatccattca ggatccgcac cgttctgcaa aagcaatcta aggaggcgtt
     6661 gttccccttc ttctttcccc ttttccatcc ctaacagcca tccatcatta tgaattcgct
     6721 ctgcaatcgt cattattcgc tccctttgtt gtggcattcg acgggtaagt tcactgataa
     6781 actcattaaa acgcgcttca tcgccagtca gtaaaatgta atttaacagc gcagttatct
     6841 ggctgtcatt agcacactct gtaaccagta atattaccag ttgatcgata agccccatca
     6901 gatcgcgctg gcgaatatgc ttttggatca actccaacag ggcgactctg cgatgctgca
     6961 caatctcgtc gtctggcacg acagtgacat ccaccagcgg gaacgctgcg gtataaagct
     7021 tccgtgcggt ggtcgggtca gcaaattcgt ccagccagca cagggaccag ggataaggac
     7081 tacggctacc gtgataaaac agcatcggga tgaccagcgg tagcggtcgg cgtttatcat
     7141 gctcgatatg gcgctgcatc accgccatgg aatatcgcat caggcgaaag gccatatgga
     7201 tatcctcgcg gctctgatgt tcaatcacta cgtaaatata accatcacct tcacgggtct
     7261 ttaccgacca cagaatatcg gagtgtagcg cccgcaattt ttcatcgacg aagctggcgg
     7321 attccagttt taagctgtcg agatcgcaca gttcacgtaa atctttgggt aagtggatct
     7381 ccataaaatc ccgcgcggtg tcagggtgcg tgagaaagga tttaaataat gcgtcatgcg
     7441 gcgtactggt cgtgaagttt gtcatggcga tccgtcacct gttgataaga tggcccgact
     7501 ttagcgacta acaaaaccat tctcacccgg taatttccca ttccccgagg ggctttccag
     7561 gctattttta ttcttctgca aacgatacat ttttccggaa acaggctcgc attttacatt
     7621 tcctgtaccc ggcagatatt cccctttcca ccgcactctt ttgattaacc gcaaatttta
     7681 attacactta aggtgtatat tttctatgca acccatcaat tcaagaggtg taatgtgctg
     7741 atgactattt gtaatcgtta tacattctga cctggagtca gaaggtattt ctctgtctgt
     7801 gtattcacag gcagtgtggt tgattacatg aaattcagta catttatcct tctcgcctgc
     7861 ctttcgtcat taccgacggt attgaatttc gttttcccca ttggggttct ccggacaagg
     7921 agttgtttgt tatgccacgt ttttttgccc gccatgccgc cacgctgttt ttcccgatgg
     7981 cgttgatttt gtatgacttt gctgcgtatc tgtcgacgga cctgatccag ccagggatca
     8041 ttaatgtggt ccgtgatttt aatgccgatg tcagtctggc ccctgctgcc gtcagtctct
     8101 atcttgctgg cggtatggcg ttacagtggc tgctggggcc gctttccgac agaattggcc
     8161 gcaggccggt gctgattacc ggggcgttaa tttttaccct tgcctgcgcc gcgacaatgt
     8221 tcaccacgtc aatgacacaa tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt
     8281 ttattgccac cgttggttat gtcacggtgc aggaggcgtt tggacagaca aaagggatca
     8341 agttgatggc gattatcacc tccatcgtac tgattgcgcc gattatcggc ccgctttccg
     8401 gcgcagctct gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcggttatgg
     8461 gttttatctc atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg
     8521 ttccatttag cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc aatcggctgt
     8581 tcctctttgg cgcagcaacc atctctttaa gctatatccc gatgatgagt tgggtggctg
     8641 tctcgccggt gatcctgatt gacgcaggcg gcttaacgac ctcgcagttc gcctgaacac
     8701 aggttccggt gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag
     8761 atccgaccga accgcgcttt atctggcgcg ccgtacccct tcaactggtt ggcctcgcgc
     8821 tgttgattat cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg ctgggcacca
     8881 gtctgtatgc tttcgggatt ggtttgattt tcccgacctt attccgcttt acgctgtttt
     8941 ccaataactt accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga
     9001 tgtctgtctc ggtcgaaatc ggccggtggc tgtggtttaa cggcggtcgc ttgccgtttc
     9061 atctgttagc cgtcgtggcg ggcgttatcg tcgttttcac cctggcggga ttgctcaatc
     9121 gcgtgcgcca acatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcccg
     9181 ccgtcaggct ctattcttaa cattatgaat aaactcattg aactcagacg cgccaaaatg
     9241 ttggcgctct ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgccg
     9301 cccaattttt gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt cggcgcgctg
     9361 gcggactggt ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgt
     9421 catacggcga ttatcccgcg taataaagac cggattggcg aaaatctcgg tcagttcgtg
     9481 caggaaaaat ttctcgatac ccagtcgctg gtggcattga ttcgacgtca cgaaccggcg
     9541 ttgttgattg gcaactggtt tagtcagccg gagaacgccc gccgcgttgg tcagcatctg
     9601 ttgcagatca tgagcggttt tcttgaactg accgatgatg cgcgtattca gcgcctgctt
     9661 aagcgcgcgg tccaccgggc gattgataag gttgatcttt ctggcaccag tgcgttgatg
     9721 ctggagagta tgaccaaaaa cgatcgtcat caggtgctgc tcgatacgct gatcgcacag
     9781 ttgatcgccc ttctccagcg cgataaatcg cgcaagttta tcgcccagca aattgttcgc
     9841 tggctggaga gcgagcatcc actgaaagcc aaaattttgc ccactgaatg gctgggcgaa
     9901 catagcgcgg agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgcgat
     9961 cgtgcgcatc agatccgcca tgcgtttgat cgcgccacct ttgccctgat cgacaagctg
    10021 aaaaacgatc ctgaaatggc agcgcgagcc gatgccgtaa aaagctatct gaaagaagac
    10081 gaagctttta accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagtg
    10141 gatatcaaca gcgaagattc tcgtgtgaaa gaacgtatcg cacgagcggg tcaatggttt
    10201 ggcgaaacgt taattgccga tgatgccttg cgggcgtcgt taaatggtca tctcgaacaa
    10261 gccgcgcacc gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat cagcgatacg
    10321 gtaaaaagct gggatgcgca ggatatgtcg cggcaaatcg agttaaatat cggcaaagat
    10381 ctgcagttta tccgtgtcaa cggtacgctg gttggcggtt gtattggact tattttgtat
    10441 ttgctgtcgc agctcccggc cttgttcccc ctcggcaatt tatagaaatc catgaaaaac
    10501 taagtggcta accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg
    10561 cctcttttca ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct
    10621 acccgccatt ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga
    10681 tctcaaggag cgcggcattc cgctggttgg cacctattgc acctttatgc cgcaagagat
    10741 cccgatggca gccggtgcgg ttgtggtttc gctatgttcc acctctgatg aaaccattga
    10801 agaagccgag aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt
    10861 cggcaaaacc gataaatgcc cctacttcta cttttctgat ctggtggtcg gtgaaaccac
    10921 ctgcgacggc aaaaagaaaa tgtatgaata catggcggag tttaagcccg ttcatgtgat
    10981 gcaactgcca aacagcgtta aggacgatgc ctcgcgtgca ttatggaaag ccgagatgct
    11041 gcgcttgcaa aaaacggtag aagaacgttt tgggcacgag attagcgaag atgctctgcg
    11101 cgatgccatt gcgctgaaaa accgcgaacg tcgcgcactg gccaactttt atcatttggg
    11161 gcagttaaat cctccggcgc ttagcggcag cgacattctg aaagtagtct acggcgcaac
    11221 cttccggttc gataaagagg cgttgatcaa tgaactggat gcaatgaccg cccgcgttcg
    11281 tcagcagtgg gaagaaggcc agcgactgga cccgcgccca cgtattttaa tcaccggctg
    11341 cccaattggc ggcgcagcag agaaagtggt gcgcgcgatt gaagagaatg gcggctgggt
    11401 tgtcggttat gaaaactgca ccggggcgaa agccaccgag caatgcgtgg cggaaacggg
    11461 tgatgtgtac gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc
    11521 gaacgatcag cgcctgcaaa tgctcagcca gatggttgaa gaatatcagg tcgatggcgt
    11581 agttgatgtg attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg
    11641 tcatgtgcgc cagcagcaca acattcctta tatcgctatt gaaacagact actccacctc
    11701 ggatgtcggg cagctcagca cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc
    11761 agtgacatat tcgattggca ttgattccgg ctcaaccgcc accaaaggga tcttactggc
    11821 tgacggcgtg attacgcgcc gtttcctcgt tccaacatcc tttcgcccgg caacagcaat
    11881 tactgaagcc tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac
    11941 cggctacgga cggcaactgg tggattttgc cgataaacag gtgacggaaa tctcctgtca
    12001 cgggctgggc gcacggtttc ttgcgccagc aacgcgcgca gtaatcgaca tcggtggtca
    12061 ggacagcaaa gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga
    12121 caaatgcgcg gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag
    12181 cgtcgaacag ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca cgagtatgtg
    12241 cacagtgttt gctgaatcag aagtgatcag cctgcgctct gcgggcgtag cgccagaagc
    12301 gattctcgcc ggagtgatta acgcgatggc gcggcggagt gccaatttca ttgctcgtct
    12361 ctcctgtgaa gcgccgattc tgtttactgg tggcgttagc cactgtcaaa ggtttaccca
    12421 catgctggaa agccatctgg gaatgccggt acaaactcac cctgatgcgc aattcgctgg
    12481 tgcaattggc gcggcggtga tcggtcagcg gcagagaaaa cgcgcatgaa agagcatctg
    12541 tttttatttc actccacggt cggcgtcata caaacccgca aggcgttaca ggcagcgggc
    12601 atgacttttc gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt
    12661 tggctgacct gtccgcctgg cgaggaaatt caatgggtga tccctgggca tacggaatca
    12721 gtttattgtc agcaggatgg tggctggcgc tgcattgcac attacgggat ttctccgcgt
    12781 taaggattca ttaagatcgc tacgtatact tctcgatatc ttattgattt gccttaaagg
    12841 gattaccgtg acaaaaagta tttcattaag taaacgcatc ttcgttattg tgattctttt
    12901 tgtcattgtg gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc
    12961 agcatcattt cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa
    13021 taatatctct tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa
    13081 acctgccgcc attgttgaaa tgaccaccaa tggtgatttg atccgcacaa ttcctctgga
    13141 ttttgttaag gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga
    13201 tgagcgcgat tacgccattt atgtgatttc actgacccca aactcggaag taaaaatcct
    13261 caaaaaaata aaaatcccgc tgcaggaatc cccaaccaat tgcggttttg aaggattggc
    13321 ctattctcgc caggatcaca ctttctggtt ttttaaagag aaaaacccga ttgaggtcta
    13381 caaagttaac gggctattaa gtagcaatga attgcacatc agcaaagaca aggcactaca
    13441 acggcaattt actttagatg atgtgtccgg cgcagaattt aaccaacaaa aaaatacgct
    13501 tctggtgctg tctcatgaat ctcgcgcact ccaggaagta acgctggttg gcgaggttat
    13561 cggtgaaatg tcactaacca aaggcagtcg aggtttgtcg cataacatca aacaggccga
    13621 aggggttgcg atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta
    13681 tcgctttacc cctcaatcat cgcattaaca ggctggcatg cttcaccttt ttgttattaa
    13741 cttgttaaca acaaaaatga caaacaaggt taaacatgcc ttcgtccacg catcccgtag
    13801 aacgcttttc tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggtt
    13861 taggccgctt cctttatacg cctatgttgc ccgtcatgat ggcggaagga tcgttttcat
    13921 ttagccagct ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc
    13981 tattttcttt tggcgcattt caccagccat cgcgcctgcg cccatttctg ttggcttctg
    14041 ccctggcgag cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt
    14101 tattgattcg cgtcctggcg ggtgtcgcca gcgccggaat gctgattttt ggttcgacgc
    14161 tgattatgca gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca
    14221 ttggcatcgc actgggcaat gaatatgttc tggcaggcct gcattttgac ctctcctcgc
    14281 aaacgttatg gcaaggcgcc ggggcgcttt ctggcatgat actgattgca cttacgcttt
    14341 taatgccctc gaaaaaacac gccatcgtac caatgccatt ggcaaaaacg gagcaacaga
    14401 taatgagctg gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg
    14461 tcgcgactta cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc
    14521 tctggacgtt agtcggctta tcaattgtgc ctggttgctt tggctggcta tgggccgcaa
    14581 aacgttgggg ggctttgccc tgcctgacgg cgaatttgct ggtgcaggct atctgtgtgc
    14641 tgcttactct cgccagcgac tcgcctctct tgcttatcat cagcagtctt ggttttggcg
    14701 gtacctttat gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgtaccgg
    14761 gaaatctcaa tcttttaggc tttgtgacgc tcatttacgg cattgggcaa atcctcggcc
    14821 cggcgctgac cagtatgctc ggcaatggaa catcggcgct tgccagcgcc actctctgcg
    14881 gcgcggcggc gttgtttatt gctgcgctta tcagcacagt gcaattattt aagctacaag
    14941 tggtcacttc gtgagagaag tttcccttac cactcaagaa actcaaccgc aactgttgat
    15001 gtcagccaga ccccgttttc cgcttgccaa aaagggatgc ctcgtttcgc catttcctgt
    15061 gctttgacgg ttaaaataac tggcgatccg tgtcttgctc ccactttgcg tgccgttgct
    15121 tcatcagcag agagatgcac gtaatggcgc tcccctgcaa tcagcccttg tttttttatc
    15181 tcgtcaagaa aacggcttgc cgtaccatgg tagagaaatt gtggcggtgt tttttcgatg
    15241 aagccaatgg cgacctgcga agtcgaatgc ccctgaacgg cgcgaataca cgtaccatca
    15301 ctggaataac taaagcgctt tttatcgctg gtagcgacaa cgatatccag taatgcgcgg
    15361 gttaaccgct tgcccgcttt ttgggcacag agtatgagct tatcgatatc cgcccatcct
    15421 tcacggtcca gcacgatgcc aatcgcctct ggtttatgtc ggaggacaaa acttaaaaac
    15481 ttgctcgtct ctgccagctc tttgtcgttg tattttgcca tctcgttctc gtccctggat
    15541 tttctgtttt tatttactga tgtcattgtc actgggttta tgaattttct gcaactcaat
    15601 cctgcgcaga acctttgcca cacagcgtca tttttccgct atgcaaaaaa cgcatagcca
    15661 gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca gctccaggaa
    15721 ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac
    15781 aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg
    15841 ctttttttct caccataatc tttttttccg gtgttttttc cgggacagac agttggtggc
    15901 gcgtttttga tttttccgtg ctgaacggat cgtttgggca actgcctggt gcgaatggcg
    15961 ccaccacctc gtttcgcggc gcaggtggca caggagctaa agatggcttt ctcttcgcgc
    16021 tggaactggc accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg
    16081 gcggattacg cgccgcgcag cagttaatga cgcccgtgct aaaaccgttg ttgggcattc
    16141 caggcatctg ttcactggcg ctgattgcta acctgcaaaa caccgatgcc gccgcaggta
    16201 tgaccaaaga actggcgcag gaaggggaaa ttaccgaacg tgataaagtg atattcgccg
    16261 cctatcaaac cagcggtagc gccatcatca ccaactactt ctcttccggc gtcgcggtgt
    16321 ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt catcctggtg tttaaatttg
    16381 tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag
    16441 gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg gatatgttta ttgatggcgc
    16501 gcgccgtggc tttactatcg ctaccaccaa cctgctgcca aacgtggtga tggcctttgt
    16561 catcatccag gcgctgaaaa ttactggcct gctcgactgg gtgggacata tctgtgaacc
    16621 cgtaatggcg ctgtggggat tgcccggtga agcggcaacc gtccttttag ccgcattgat
    16681 gagcatgggc ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg
    16741 ccacgatgtc accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt
    16801 cggtcgctgc ctgggaacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt
    16861 ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt
    16921 taccatgatt gattattccg cagccggttt taccctgctg cagggagcgc atttgtatgc
    16981 gccggaagat cggggaattt gcgatgtcct catcgctaac ggcaaaatta ttgccgttgc
    17041 cagcaacatc ccttctgaca ttgtaccaga ctgcacggtt gtcgatctca gcgggcagat
    17101 cctctgccca ggttttattg atcaacacgt ccatttgatt ggtggtggcg gcgaagcagg
    17161 tcccacgaca cgcacgccgg aagtggcgtt aagtcgtctg acggaagcgg gcgtcacgtc
    17221 agtggttggt ctgctgggta ccgactctat ctctcgccac ccggaatccc tgctcgccaa
    17281 gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt
    17341 cccttcccgc accattacag gttccgtgga aaaagacgtg gcgattatcg atcgcgtgat
    17401 tggtgtgaaa tgcgccatct ctgatcaccg ttctgccgca cctgacgttt atcacctggc
    17461 taatatggcg gcggaatccc gcgttggcgg tttgcttggc ggtaaacctg gcgtcaccgt
    17521 gttccacatg ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg
    17581 cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgttc cgttgtttga
    17641 gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgac atcaccagta gcattgacga
    17701 accggtcgcc cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg
    17761 cgtcaccctc agctccgacg gcaacggtag ccagccgttc tttgatgacg aaggaaattt
    17821 aacccatatc ggtgttgcag gttttgaaac attgctggaa accgtgcaaa tgttggtcaa
    17881 agactatagc ttcagcctta gcgatgccct gcgcccgctc accagtagcg tagccggttt
    17941 ccttaacctg accgggaaag gcgaaattct gccaggcaat gacgcagact tactggtcat
    18001 gacgcctgaa ttgcgcattg agcaggtata cgctcgcggc aaactgatgg tcaaagacgg
    18061 caaagcctgc gtgaaaggaa cgtttgaaac ggcataaccg gttgtcagcc gcccgaatgg
    18121 acgtagaatg cccgcaaggg cggctgacag agtataacgt aatggatgac tgtggtgcgg
    18181 ttttgcataa cattgaaacc aaatggcttt atgattttct gaccctggaa aaatgtcgca
    18241 atttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc
    18301 gtgcactgga acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac
    18361 tctcggaaca agggaaaatc ttccattcgc agatccgcca tctgttgcaa cagttagaaa
    18421 gcaacctggc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc aagatagccg
    18481 ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg ccgccgctct
    18541 ttacctgggc aattgaagcc attgatgtcg atgaagcggt cgataaactg cgcgaagggc
    18601 aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca
    18661 ttcgcttatt tgaatctcaa ttattccccg tctgcgccag tgacgaacac ggagaagcac
    18721 tgtttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg
    18781 ggcgattgat taatcgcacc ctgacgcgcc acagtgagtt gagtttcagc accttttttg
    18841 tctcttcgat gagcgagcta ttaaagcagg ttgccctcga cggctgtggg attgcctggc
    18901 tgccggaata cgctatccaa caagaaattc gcagcgggca gctcgttgta ctgaatcggg
    18961 aagaactggt gatcccgatt caggcctacg cataccggat gaatacccgc atgaatccaa
    19021 ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgaacctggt tgaaagtttt
    19081 ctgcagaaaa tcttccggcg ctgggaatta tgattgaggt tcctgccgca gtcatgattg
    19141 cagacaaatt agccagtgaa gtcgatttct tttccatcgg caccaacgat atgacgcaat
    19201 atattatggc ggcagacaga ggaaatagca ccgtcgcgaa actggtcgat tattgtaatg
    19261 acgcggtgat taacgctatt gctatggtgt gtcaggccgg acgtaataat gaaattccgg
    19321 tcagtatgtg cggcgaaatg gctggagata tccagcaaac agcacgactg ctcaccatgg
    19381 ggattgataa gctcagtgcc agtccatccc ggcttccggc cttgaaagca gcaatcagag
    19441 cctcccacta attgtgacct aacgcgatac gtcttgcgcg tatcgcgttt cccgtcattt
    19501 actgcggcat cgtgcgatgc cagttcccct tctgccagac caggtcaaac gttagcggct
    19561 catcatcgtg gtacagcatc atctccagac taatggccaa ctcgtggcaa taggcttctt
    19621 tcagattgac ctgcaatgcc gcaatacgtg gctcatgaaa taaaagcgcg gaacgtaact
    19681 ggcggcaata ccaactctgg agaagcttgt cgctctgatt atgcccttgc cactcaggca
    19741 tgcctgaaac aagtaatatt ggtagcggtg aatggggaga actaaaaagt tgaaagagat
    19801 cccggcatac ggaatcacgc agcgataacg tattgtgtcc tgtactttca ggtaataccg
    19861 tctgaactga ttggcgcatc attttgcgca ctcctgacgt ttagtggcat ctgtatcctg
    19921 ccctggctgc cggacaccac tgggttgatg ctcatattcc ataatcattc ctttatgctc
    19981 attgcgtccc tggcactttc ataagggatt tataagatcc gccaaagata aaacactgta
    20041 cagaaaagaa tatccactga ctttttattt aaaaaggtat catttgacaa aagtcattaa
    20101 acataacgga aatacgttaa catattctga ttattccgaa taatttctca gcgttaaact
    20161 gatatataat tattccaaaa caatatatcg tacgtaatga ttaatttacg cataatatat
    20221 tctgcagaac ataattaact attcagggaa catacatagt gcaaagcggt tcaactttgc
    20281 accagaaatg agggatcagg cgtcgacttt atccagcggc catgaatcaa acagatagcc
    20341 gtcaaaataa atatcgtcaa cgttcgagaa ttccagcaga cgttgtttaa cgttttccag
    20401 atgctgccac attgccagct tcgccgcacg ggcatctttt ttgattaacg cggcgaggat
    20461 ctgtttgtga tcgcccaacc actctttgcg atacaggctg tcatcgagat ggctgtgcaa
    20521 ttggatccac attggattgt tttcccgcca ctgccaggac tgacggaata actccaccag
    20581 catgctgtta tgcgttgctt cggcaatggc gagatggaac tgcatatcac cgctttcgct
    20641 gctacccggc gcgctggaag ccagttcacg ctcttccagt tgcaatgcct gacgcatttt
    20701 gacgatatct tcgcgggtag cctgcaaagc ggcaaattcc gcgatgttac tttccagtaa
    20761 ctgacgcgcc tgtaacagct caaaaggacc ggcatcatta cagacgttgg catccggact
    20821 gtctgtgttg tggctgcctg agctatcaag aacataaata ccggcacccc ggcgcacttc
    20881 caccagccct ttgatctcca gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc
    20941 aagcatttct gcaatttcac gctccggtgg cagccgttcg ccaggattgt acggcgtctt
    21001 tatgatcaga tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc
    21061 agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa attttttccg gatgcggcgt
    21121 gaacgcctta tccgacctac aacgggcaat tttacggcac ccgttaagct caaactatac
    21181 cattagcact ggatgttggc acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa
    21241 tattagtttg attgaccagt aagtctgtag ggtaatcagt tagttacagc gcagccacac
    21301 actcgcgcgc accgcgctca cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt
    21361 cggcgttctg cggcagatca tcggcaaaga taccgctcag gcccagcagt gctttcacgc
    21421 ggtctgcact ctgatactgc gcgttgatct tctggaactc cgccagcatc gggtcaacca
    21481 cgtcaatggc attaccctgc tcatccacgc cctgggtgta acgcatccag cccgccacgc
    21541 ccagcgccag gtgacgccag ctaccgccgt tttgcaggtg cagacgcacc gggtccagca
    21601 gacgctgcgg taacttctgg ctgccgtcca ttgcaatctg ccaggtacgg tgacgcagag
    21661 acgggttgct gaaacgctcg atcagcagcg tcgcataagc gttcaggtct gtaccttccg
    21721 gcatcgacag cgttggcgct tgttcctgca tcatcagggc aaaggccgct ttgcgataag
    21781 ccgggttagt catggtgtcg gcaatagttt catagccgcc gaggtaaccg aggtacgcca
    21841 ggaaagagtg gctaccgttc agcatacgca gcttcatcat ttcgaacggt acaacatccg
    21901 caacaaactg cgcgccgact ttatcccagt ccggacgacc attaacgaag ttatcttcaa
    21961 ttacccactg acggaacggt tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt
    22021 cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca accatggtgc
    22081 acgggaaggt gacgttctct tcaatccatg ccgccagttg cgggtcacgc gcctgagcca
    22141 gcccaagaac ggcaaccttc gccacatgac cgttttcacg cacgttatcg caggacatca
    22201 ccgtaaatgc tttcagccct ttttcacgac gcagacgcag ggcttctacg atgtaaccaa
    22261 tcgcagactt cggcgcagtc gggttttcca gatcgtgctt gatcagcggg ttattgagat
    22321 ccagttgacc gcttgccgca tctgcgcagt agcctttttc cgtgaccgtc agagagacaa
    22381 tcgccgtctg cggacgcgcc atcgcgttga gaataccttc gcaaccgtcg atttccgggt
    22441 gcagggcttc tttcatcgaa ccgataattt tcagctcggt gctttctgcg cctttttccg
    22501 ccacggtgta cagcagttgc tgttttttca ggttttcgat cagcacgcgg tcgttgcctg
    22561 gcatcaggtt aacttcgcag atcccccagt cgctatccgt gctttccagc aggtgatgag
    22621 tgtacagcgc ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt
    22681 ccagacgaga atgatcccat gacgggcggg caaccggcag attgctgtca acaatagtag
    22741 tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc aaggaagaag gaaccggcac
    22801 ggcgcgcagc catgccggtg gatattaacg gctaaagaaa gcgcgctgga tcgccagttc
    22861 gacaccgcga acttcggcca ggcctttcag acgaccaatt gcggagtaac caggattggt
    22921 tttcttcttc aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc
    22981 ttctttgcct tccgctttac gacggtgttc ttcttcaaca atcgctttca ccacttcgta
    23041 catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg
    23101 catggtggag cgcagatggg tgaagtaaat acgcggaccg aactgcttga tcatatcaac
    23161 cagatcgttg tcagcacgca cgccgtagga accggtgcac atggtgaaac cgttcgccat
    23221 gctgtttacg gtatcaacca tccactgcat atcttcaatg gtggaaacaa tgcgcggcag
    23281 gccgaggatc gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc
    23341 agcaactgga ataatcgctt tcaggaagac agcgaagttt tcgcgcagct tcgctttgtc
    23401 gatatctttg tacagctcca ggtgcttacg gaactggtcg agggtatacc cttcttccgc
    23461 acctggcaga ccgacaatga tgttacgggt cagacgcgct ttgtcttcgt cgctcatagt
    23521 ggcgaagcga acagctgcct gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg
    23581 acgcttcagg atatgcattt cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt
    23641 ggagccgtct ggcagcacgt attcgaggtc agtacgggtc cagtcgagca ccggcatgaa
    23701 gttgtagcac acggtgcgaa tgccgcactg cgccaggttg cgcagggtct gctgatagtt
    23761 agcaatccac tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct
    23821 ttcaacgaca gaccacacca ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc
    23881 ttctacggac catacttcgc cgttcgggat atggtgcagc gcggtaacca cgccagttgc
    23941 gcccgcctga cggacatcag ctaaagaaac cggatcgttt gggccgtacc agcgccaggt
    24001 ctgttccatg atttttcctc tttcacatag tgggttccac agcaaagaga cgtggagccc
    24061 cggagttgtt taggtatccg gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg
    24121 agatggcgta taagttctac cttttggtaa gacatgtcaa cccgaaatct gaaattggtt
    24181 aaccacatca caagaatttc actctcctgg ttgttgctgc aaattgcagc aaagggtaat
    24241 cgatggttat tgatcagagg atgtgacatt catcgcaaca atggttgacc aatttacata
    24301 acatgtcgcc caaaataaca acggttcaac caggtaaggt aaaacgttat gcatgtgctt
    24361 aacattctct gggtggtatt cggcattggt ctgatgctgg tactgaattt gaagttcaaa
    24421 atcaattcaa tggtggcttt gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg
    24481 gatttgatgt cgctgctgca caccatgaaa gcgggcttcg gcaacacgct gggggaactg
    24541 gctatcatcg tggtgttcgg tgcggtcatc ggtaaattga tggtcgactc cggcgcggct
    24601 caccagatag cacatacgct gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg
    24661 attatcatcg gcctgatttt cggtctggcg atgttttatg aagtggcctt tatcatgtta
    24721 gcgccgctgg ttattgttat tgccgccgaa gctaaaattc cgttcctgaa actggcgatc
    24781 ccggcagtag cagctgccac taccgcacat tcactgttcc caccgcagcc gggtccggtg
    24841 gcactggtga atgcttatgg cgcggatatg gggatggttt atatctatgg cgtactggtg
    24901 acgatcccaa gtgtcatctg cgcaggtctg atcctgccga agttcctcgg caatcttgag
    24961 cgcccaacgc catcattcct gaaagcagat caaccggtag atatgaataa tctgccctct
    25021 ttcggcgttt cgattctggt gccgctgatc ccggcgatca ttatgatctc taccaccatc
    25081 gccaatatct ggctggtaaa agatacccct gcctgggaag tggttaactt tatcggttcc
    25141 tcgccgattg caatgtttat tgcgatggtg gttgcattcg tactctttgg caccgcgcgt
    25201 ggtcatgaca tgcagtgggt gatgaacgct tttgaaagcg cggtgaaaag cattgcaatg
    25261 gtgattctga tcatcggtgc gggtggcgtg ctgaagcaga ccatcatcga caccggcatt
    25321 ggcgacacca tcggcatgtt gatgtcccac ggcaatatct cgccctacat catggcatgg
    25381 ctgatcaccg tactgattcg tctggcgacg ggtcagggtg tcgtttcggc gatgaccgct
    25441 gccgggatta tcagtgctgc aatccttgat ccagcaaccg gtcagctggt tggcgtgaat
    25501 ccggcgctgc tggtgctggc gacggctgcg ggttccaaca ccctcacgca cattaacgat
    25561 gcatctttct ggttgttcaa aggttacttt gacctgtcag taaaagacac gttgaaaacc
    25621 tggggtctgc tggagctggt caactccgtg gttgggctga ttattgtgtt gattattagc
    25681 atggtagcgt aaaagaacac cggagcctgc caatggcagg ctctgttcgg attgtcggta
    25741 aagtgcgctt tgtttatgcc agatgcgacg ctgacgcgtc ttatctggca tacaaagggc
    25801 taacgtgcag gttttagctt caggtaatat tgcgtacctg cattagcaat gccctgtgat
    25861 ttctttattg ataaacaaaa gtaacgccaa taatcgattg cacattccct gcagtcacct
    25921 gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa gtccccactg
    25981 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
    26041 cgacgccctg tgctggtgaa aacgaggcgg tattggtgaa aatcgagttg cccgcatctg
    26101 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
    26161 gaatcggcac cgaaccggga tagtccggca gagtaacggt gacatcacga gcagaaacat
    26221 cacagccgcc agtaggcacc accacatcat tattggcgta aatattccac acaaactgga
    26281 aatcatcgct gttatagttg ttggtctgtc gcaaaattag cacggcaatt aatgagccag
    26341 ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
    26401 gcttatccgt tctcgaatta taaacaacgc gcggcgtttc gctagtggtc ggaaatggat
    26461 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
    26521 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
    26581 tttgcgtcga aagatccacg accaggtttt gccccacatt cacggcaggc gcaaggttta
    26641 cataaacatt agcgctgcca ccgccaatag ggatagcggt gccattggcg gttttacagg
    26701 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg taaataactc
    26761 gtttcattac aatcatctct tcggcttcag ctgtaggtat aggtgatgct aattactgcc
    26821 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
    26881 tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccact attcaatgtg
    26941 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
    27001 tatccggtac tgtcggctgc cccgctgaag ccggcggtga ccctcgacgt tcccaccgga
    27061 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga
    27121 ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac cgtgcacggt
    27181 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt
    27241 gcgagcgcca acattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc
    27301 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
    27361 acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg
    27421 gtcgtccacg aaatcgcgga cgatggggca ttaaggggta tctgattttg ttgctcattc
    27481 agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc aagcaggttg
    27541 gcattgtggc tatctgcaac accggtaaac ccaactttta cggcagaaac ggcattacca
    27601 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gttgttaaat
    27661 tgcttcaccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
    27721 cagccattat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaagcgtga
    27781 cttaccgcca gccacaaaaa agcgcaaaga agataaaaag gtttgtttct catcacgcct
    27841 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
    27901 tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
    27961 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
    28021 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
    28081 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgggtg
    28141 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc
    28201 agcgctactc tgttttcccg atattcagtg gcataaggca gcacggcata accgcgccag
    28261 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
    28321 agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
    28381 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatgttg
    28441 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
    28501 tcacctcccc cggcgtaacc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac
    28561 gtaccgtata caccggccag attggtcatc cgaccgttga gatcgtgtga catgctgtaa
    28621 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
    28681 atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg
    28741 ctatagctga gcgtccagtt gatatcttcg aatgcagtat ttaaaccagc ctggaattgc
    28801 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
    28861 gagcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgaga
    28921 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
    28981 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatacccact ggtcgaataa
    29041 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt ataaagaaaa
    29101 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
    29161 tccacagaca gagcgcccag tgcccccata tttttcccga taccgaaatt aaacgcacga
    29221 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
    29281 aatgtactct ggaaaaagcg gggtttttcc tgttgcgcat ttccactacg gtattctcct
    29341 gccgtaatgg aataacgtgt atgcccttca cgctgcaaaa gcgggactga cgaatagggt
    29401 acggtaaaaa tctgcgtact gccgtcagcc tctttaatcg ttacctgcaa gtcaccacta
    29461 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
    29521 atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
    29581 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
    29641 ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc
    29701 cgggaacgta acggaattat gtctcgctca agccaggtat tgatatgctg ccatttattt
    29761 ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc
    29821 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
    29881 attcgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
    29941 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
    30001 atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc atggatcatt
    30061 gaggttaatg gcacgcaggc atcatccgcc agcagattca taccggagac agaagccgta
    30121 ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg
    30181 tcgcccgtgt taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg
    30241 acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca
    30301 gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt
    30361 gcctgtgcgg caaaagcaca ggcgacaaag agccggacaa aaaaaccagc caaacgatgc
    30421 ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
    30481 attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga aaaattcccc
    30541 ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt
    30601 cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga
    30661 ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa
    30721 tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct
    30781 gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagctt aatgcggctg
    30841 ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct
    30901 ttaacgttca tccagaataa actttcccga tcctgtggca attggttatt tgttgcatca
    30961 agaatacgta aggtattctc tctttttccc ttcatcgcaa acagaggtgg cgtcacgata
    31021 aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa ataggtgcta
    31081 ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg
    31141 cgggtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac
    31201 atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt
    31261 ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt
    31321 aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccgacgccc ggtagcacga
    31381 tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttgctgga
    31441 ggacgattaa tcggtacgag gtttccttca tcatcaaata acgctacgcc aatattggtg
    31501 gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg
    31561 tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca
    31621 aaaggcaccg gtgcgctatc ttcccctacc gcatgaaacc ggttactgct gattttcccc
    31681 atattgaccg tcatttgttt atcaccagct tcaactcggc aagtttccgc aataatgacg
    31741 ccctgaaatt gcatatttcc gccgggcagc gtggtattcc atttatttcc ggccagagca
    31801 aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc
    31861 gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata
    31921 ctgaaccttg aaggtcgcat ccgcattagc agcacccggg gttgcctcgc cgattgcata
    31981 ataacgcgcc tggaacggaa tggtgttggt accgttattc aaggttgttt gtgcactaaa
    32041 tgtcgcaccg tccagcgcca gcgcagcacc cgttctgtcc aggatctgca caccaacttt
    32101 tgttgcgcta cccgcagctg aactctgcaa agctaatacg ttaggatgag ttccatcaat
    32161 tgcggtacca aggaaggcaa tagcagcttt gtctgcaaca gtagtgtcac aatcgttcag
    32221 ttgaatgtta aaaccgacag cagaactggt tgctccttct tgtgccagcg atgctgtacg
    32281 aacctgccct aactgaacag tttgatcaac agagcctgca tcaactgcgc aagcggcgtt
    32341 aacaacctcc cctttaaagt gaacggtccc accatttacc gtcgtgggtg tagtttcagc
    32401 cagagccgct gtagaactca gggacagagc cgacagaaca acaattgcca gagttttaat
    32461 tttcatactg ctttcctttc aaaaaactat ttctaaatcg acatgggcag tcgttctgta
    32521 cactttgttt tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga
    32581 aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta
    32641 tttgttggct taatattcta ttgttatctt tatttatag
//
LOCUS       DAZGTJ010000055        32619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000055 DAZGTJ010000000
VERSION     DAZGTJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.055"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..407
                     /locus_tag="ACHK8E_003070"
     CDS             <1..407
                     /locus_tag="ACHK8E_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP0345303.1"
                     /translation="EQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDEQRR
                     ELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAEL
                     AAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            422..1279
                     /gene="menB"
                     /locus_tag="ACHK8E_003071"
     CDS             422..1279
                     /gene="menB"
                     /locus_tag="ACHK8E_003071"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP0345304.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1279..2241
                     /gene="menC"
                     /locus_tag="ACHK8E_003072"
     CDS             1279..2241
                     /gene="menC"
                     /locus_tag="ACHK8E_003072"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP0345305.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2238..3593
                     /gene="menE"
                     /locus_tag="ACHK8E_003073"
     CDS             2238..3593
                     /gene="menE"
                     /locus_tag="ACHK8E_003073"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP0345306.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3703..3969
                     /gene="pmrD"
                     /locus_tag="ACHK8E_003074"
     CDS             3703..3969
                     /gene="pmrD"
                     /locus_tag="ACHK8E_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP0345307.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3963..4349)
                     /gene="arnF"
                     /locus_tag="ACHK8E_003075"
     CDS             complement(3963..4349)
                     /gene="arnF"
                     /locus_tag="ACHK8E_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP0345308.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4349..4684)
                     /gene="arnE"
                     /locus_tag="ACHK8E_003076"
     CDS             complement(4349..4684)
                     /gene="arnE"
                     /locus_tag="ACHK8E_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP0345309.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4681..6333)
                     /gene="arnT"
                     /locus_tag="ACHK8E_003077"
     CDS             complement(4681..6333)
                     /gene="arnT"
                     /locus_tag="ACHK8E_003077"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP0345310.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6333..7223)
                     /gene="arnD"
                     /locus_tag="ACHK8E_003078"
     CDS             complement(6333..7223)
                     /gene="arnD"
                     /locus_tag="ACHK8E_003078"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP0345311.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7220..9202)
                     /gene="arnA"
                     /locus_tag="ACHK8E_003079"
     CDS             complement(7220..9202)
                     /gene="arnA"
                     /locus_tag="ACHK8E_003079"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP0345312.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9202..10170)
                     /gene="arnC"
                     /locus_tag="ACHK8E_003080"
     CDS             complement(9202..10170)
                     /gene="arnC"
                     /locus_tag="ACHK8E_003080"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP0345313.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10174..11313)
                     /gene="arnB"
                     /locus_tag="ACHK8E_003081"
     CDS             complement(10174..11313)
                     /gene="arnB"
                     /locus_tag="ACHK8E_003081"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP0345314.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11621..12223
                     /gene="ais"
                     /locus_tag="ACHK8E_003082"
     CDS             11621..12223
                     /gene="ais"
                     /locus_tag="ACHK8E_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP0345315.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12262..12687)
                     /gene="nudI"
                     /locus_tag="ACHK8E_003083"
     CDS             complement(12262..12687)
                     /gene="nudI"
                     /locus_tag="ACHK8E_003083"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0345316.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            12966..13508
                     /gene="yfaZ"
                     /locus_tag="ACHK8E_003084"
     CDS             12966..13508
                     /gene="yfaZ"
                     /locus_tag="ACHK8E_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP0345317.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            13608..14810
                     /gene="yfaY"
                     /locus_tag="ACHK8E_003085"
     CDS             13608..14810
                     /gene="yfaY"
                     /locus_tag="ACHK8E_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP0345318.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            15030..15812
                     /gene="yfaX"
                     /locus_tag="ACHK8E_003086"
     CDS             15030..15812
                     /gene="yfaX"
                     /locus_tag="ACHK8E_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP0345319.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            15827..17032
                     /gene="rhmD"
                     /locus_tag="ACHK8E_003087"
     CDS             15827..17032
                     /gene="rhmD"
                     /locus_tag="ACHK8E_003087"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFP0345320.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            17089..18378
                     /gene="yfaV"
                     /locus_tag="ACHK8E_003088"
     CDS             17089..18378
                     /gene="yfaV"
                     /locus_tag="ACHK8E_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345321.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            18395..19198
                     /gene="yfaU"
                     /locus_tag="ACHK8E_003089"
     CDS             18395..19198
                     /gene="yfaU"
                     /locus_tag="ACHK8E_003089"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP0345322.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(19239..19472)
                     /locus_tag="ACHK8E_003090"
                     /pseudo
     CDS             complement(19239..19472)
                     /locus_tag="ACHK8E_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19473..20468)
                     /locus_tag="ACHK8E_003091"
     CDS             complement(19473..20468)
                     /locus_tag="ACHK8E_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP0345323.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(20661..21851)
                     /gene="glpC"
                     /locus_tag="ACHK8E_003092"
     CDS             complement(20661..21851)
                     /gene="glpC"
                     /locus_tag="ACHK8E_003092"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP0345324.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(21848..23107)
                     /gene="glpB"
                     /locus_tag="ACHK8E_003093"
     CDS             complement(21848..23107)
                     /gene="glpB"
                     /locus_tag="ACHK8E_003093"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP0345325.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(23097..24725)
                     /gene="glpA"
                     /locus_tag="ACHK8E_003094"
     CDS             complement(23097..24725)
                     /gene="glpA"
                     /locus_tag="ACHK8E_003094"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP0345326.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            24998..26356
                     /gene="glpT"
                     /locus_tag="ACHK8E_003095"
     CDS             24998..26356
                     /gene="glpT"
                     /locus_tag="ACHK8E_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP0345327.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            26361..27437
                     /gene="glpQ"
                     /locus_tag="ACHK8E_003096"
     CDS             26361..27437
                     /gene="glpQ"
                     /locus_tag="ACHK8E_003096"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0345328.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            27640..28290
                     /gene="inaA"
                     /locus_tag="ACHK8E_003097"
     CDS             27640..28290
                     /gene="inaA"
                     /locus_tag="ACHK8E_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP0345329.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(28344..28598)
                     /gene="yfaE"
                     /locus_tag="ACHK8E_003098"
     CDS             complement(28344..28598)
                     /gene="yfaE"
                     /locus_tag="ACHK8E_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP0345330.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(28598..29728)
                     /gene="nrdB"
                     /locus_tag="ACHK8E_003099"
     CDS             complement(28598..29728)
                     /gene="nrdB"
                     /locus_tag="ACHK8E_003099"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0345331.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(29794..32079)
                     /gene="nrdA"
                     /locus_tag="ACHK8E_003100"
     CDS             complement(29794..32079)
                     /gene="nrdA"
                     /locus_tag="ACHK8E_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0345332.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            32207..32350
                     /gene="ypaB"
                     /locus_tag="ACHK8E_003101"
     CDS             32207..32350
                     /gene="ypaB"
                     /locus_tag="ACHK8E_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP0345333.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
BASE COUNT         7729 a         8158 c         8736 g         7996 t
ORIGIN      
        1 ctgaacaacg cacggaacgt cagcgttccg atcgccaatg ggcgcagcgt tttcgcacag
       61 aaccgttaac ggcggtattt gctgactggt atcaacagcc tgtttttgcc tcactcaatg
      121 acgaacaacg ccgggagctg gttgcgctgc gcagcaacaa taatggcgca acacttgccg
      181 ccatgctgga ggcgacttct ctcgccgtcc agcccgattt acgtgctaac cttagcgccc
      241 gcacatttgc gttttattat ttatgtggtg aacgtgacag caaattccgc gccctggcgg
      301 cggaactggc tgccgaatgc catgtcattc ctcgcgccgg acataacgcg caccgggaaa
      361 atcccgctgg cgtaatcgca agtctggcgc agatcttgcg tttctgacta aaggacacaa
      421 tatgatttat cctgatgaag caatgcttta cgcaccggtt gaatggcacg actgctccga
      481 aggttttgag gacattcgtt atgaaaaatc caccgacggt atcgcaaaaa tcaccattaa
      541 tcgtccgcag gtgcgcaatg ccttccgtcc tctgacggta aaagagatga tccaggcact
      601 ggcagatgcg cgttatgacg acaacatcgg cgtgatcatt ctgactggcg caggcgataa
      661 agcgttctgc tccggcggtg accagaaagt gcgtggtgat tacggcggct ataaggatga
      721 ttccggcgta catcacctga acgtgctgga cttccagcgt cagatccgta cctgcccgaa
      781 accggttgtc gcgatggtgg ctggctactc cattggtggc ggtcacgttc tgcacatgat
      841 gtgcgacctg actatcgcgg cagataatgc catcttcggt cagactggcc cgaaagtcgg
      901 ttccttcgac ggcggctggg gcgcttccta catggcgcgc atcgtcgggc agaaaaaagc
      961 gcgtgaaatc tggttcctgt gccgtcagta cgacgcgaaa caggcgctgg atatgggcct
     1021 tgtaaatacc gtggtgccgc tggcggatct ggaaaaagaa accgtgcgct ggtgccgtga
     1081 aatgctgcaa aacagcccga tggcgctgcg ctgcctgaaa gctgcactga acgccgactg
     1141 tgacggtcag gcgggtctgc aggagctggc gggcaacgcc accatgctgt tctacatgac
     1201 ggaagaaggt caggaaggtc gcaacgcttt caaccagaaa cgtcagcctg atttcagcaa
     1261 atttaaacgg aatccgtaat gcgtagcgcg caggtatacc gctggcagat ccccatggac
     1321 gcgggggtgg ttctgcgcga caggcggtta aaaacccgcg acgggctgta tgtttgcctg
     1381 cgtgagggcg agcgcgaagg gtggggggag atctccccac taccgggctt cagccaggaa
     1441 acctgggaag aggcacaaag cgggctgctt gcctgggtaa ataactggct ggcaggtgat
     1501 agcgagctac cgcaaatgcc ttccgtcgcc tttggcgtaa gctgtgcatt ggcggagctg
     1561 gcagacacgc tcccgcaggc ggccaattat cgtgcggcac cactgtgtaa tggcgatccg
     1621 gacgatctga tcctgaaact tgccgatatg ccgggcgaga aagtggcgaa ggtcaaagtg
     1681 ggattgtacg aagccgtgcg cgacggcatg gtggtcaatc tgttgctgga ggcaattccg
     1741 gatctgcatt tgcgtcttga cgcgaaccgc gcctggacgc cgctgaaagg ccagcagttt
     1801 gccaaatacg ttaacccgga ttatcgcgac cgcatcgcgt ttctcgaaga gccgtgcaaa
     1861 acccgcgatg attcccgtgc gtttgcccgt gaaaccggca ttgccattgc ctgggatgaa
     1921 agcctgcgcg agccggattt tgcctttgtg gctgaagagg gcgtgcgcgc ggtagttatc
     1981 aaacccacgc tcacgggcag cctggagaaa gtacgcgagc aagtgcaggc ggcgcatgcg
     2041 ctggggctga cggcggtgat cagttcttcc attgagtcga gcttaggtct aacgcaactg
     2101 gcgcggattg ctgcctggtt aacgccagac accattccgg gactggacac gctggatctg
     2161 atgcaggcgc agcaggttcg tcgctggccg gggagcctgc tgccgttagt ggacgttgat
     2221 gcactggagc ggctgttatg atcttctctg actggccgtg gcgtcactgg cggcaagtgc
     2281 ggggagaagc catcgcctta cgtcttaatg acgagcaact caactggcgc gagctttgtg
     2341 ctcgcgtcga tgaattagcc tccagctttg cgatgcaggg tgtggttgag ggcagcggcg
     2401 tgatgttgcg ggcgtggaat acgccgcaaa cgctgctggc ctggctggcg ttactgcaat
     2461 gcggggcgcg ggtgctgccc gtgaatccac agctgccgca accgttactt gaggaattgc
     2521 tccccaatct tacgctgcaa tttgcgctgg tgccggatgg ggaaaacacg tttccggcat
     2581 taacgtcgct gcacattcag cgggttgaag gcgcacatgc cgctacgtgg cagccgacgc
     2641 gtctgtgctc aatgacgttg acctcgggtt ctaccgggct gccgaaagcc gctgtccata
     2701 cttatcaggc ccatcttgcc agtgccgaag gggtgttatc gttgattccg tttggcgatc
     2761 acgatgactg gttgctctct ttaccgctgt ttcacgtctc cggtcaggga attatgtggc
     2821 gctggttata cgctggtgcg cggatgacgg tgcgtgataa acagccattg gagcaaatgc
     2881 tggcaggctg tactcacgct tcactggtgc caacacaact ctggcgtttg ctggttaacc
     2941 gcagttccgt ttccctgaaa gcggtgttac ttggcggcgc ggctatcccg gtcgagttga
     3001 cggaacaggc gcgcgagcag gggattcgtt gcttttgcgg ctatggtctg accgagtttg
     3061 cctccacggt gtgtgcgaaa gaagccgacg gtctggcaga cgttggttcg ccgctgccgg
     3121 gtcgggaagt gaaaatcgtt aatgatgaag tgtggctgcg ggctgctagt atggtagaag
     3181 gttactggcg taacgggcaa ctggtttcac tggttaatga cgaaggctgg tacgctacgc
     3241 gcgatcgcgg tgagatgcat aatggcaagc tgaccattgt cggacgttta gacaatctat
     3301 tcttcagtgg cggagagggt attcagccgg aagaagtcga gcgcgtaatt gctgcacatc
     3361 ctgcggtttt gcaggtgttt atcgtccccg ttgccgacaa ggagtttggt catcgaccgg
     3421 tggcggtggt ggagtatgac cagcaaaccg ttgatcttga cgaatgggtg aaagacaagc
     3481 tggctcgttt tcaacaaccg gtgcgctggc tgaatctgcc gccggagctg aaaaacggcg
     3541 gtattaaaat ttcacgtcag gcgctaaaag agtgggtgca acgccagcaa taattaaccc
     3601 tccgtttcac ttctcatttc tgccattcca ttgccgttga taaagagttt atctgttaat
     3661 ctgtaaattg atgtgaaaac tcttagcaaa caggataatg caatggaatg gctggtcaaa
     3721 aaatcgtgct gcaataaaca agataacaga catgtaatta tgctctgcga tgctggcggc
     3781 gcgataaaaa tgatcgccga agtgaagagt gacttcgccg tgaaagtggg ggatttactc
     3841 tcgcctttgc agaatgcgct ttactgtatt aatcgtgaaa agctgcacac ggtgaaagtc
     3901 ctttctgcaa gctgttatag ccccgatgag tgggaacggc agtgtaaagc ggcagggaaa
     3961 actcagtaac gttgttttgt tgtgggcagg aaaatcagca tcaacccgct cataatacag
     4021 gctactccca gtagtgcttt cagcgaaaaa gtcccttccc agccgggaag taccattgaa
     4081 gcaatccata ccagcacata actcatactg agcaaggcat aggctttgct tagtgcaagt
     4141 ttatgtagtg ttttatacca acaaaaaacg gaaagcagat aacccagcag cccgagtaac
     4201 agtatccggg catccaggcc aaaagctaac aacgccgcga taaaatccca taagtgcgtc
     4261 atcggcggca gatgactcgc cgcaaaaccc aggcttaatt gcgcagcgga ggcgataatg
     4321 acgctgaata atccccacat caggcccatt acacggtact cccgaggatc acaatgccac
     4381 caataatgaa cgccacccca caccagtgac gcggcgatac cggttcgtgc cacagtttta
     4441 ctgcagccag cgtcacccag acaaaattca gacttaacat cgggtaggca atgcctaccg
     4501 gtacgttctg caagaccagc agccagagca ccatggcaag accaagacaa gccagcgcca
     4561 gtcccagcca cagcacgata tgtttgcgcc gtttgtttat cgccacaaag caggttgcct
     4621 gtttctgaca caactgcccg gcaacgctaa gcaagctggc aaagactaat gttagccaga
     4681 tcatttggga cgatactgaa tcagcaccag acgctcctga cgatcgatgg catctgcggg
     4741 cggaatggcg agactgttga tatcttcatt gcggtcaacc gaaagcacga gtgtaataat
     4801 cccctcctga cgatgttgat taagccagtt tgcgaactca tcaccgctga caaatctccc
     4861 tttcgcatcc ggataattaa ggccgtattt caactcacct gtctggcgat acatgatgat
     4921 gtcatcgcgt tgcaggctcc atgccagacc tgcggcaaca ccgacgctat cagtaagaat
     4981 atagcggctt ggctgaagtg attcttgtgt catctcgaca aaaaattgcg gatgttttcc
     5041 ttccatgact ctgtcaggaa ttgaaaatcc taccagcaac gccagcccca gcgggcaaag
     5101 cgcggcaaaa gaccaggtct tttcgacgtt tgttaaggtg taccagccga aaaatgccca
     5161 tagcgaaaaa atcgaccagg cacaaaagac tttatagctc tcgaaggttt gccacaccgg
     5221 cgtgttcatt gctccccagg gggagaccac aaatgtggca ataatgccag tgacgccaaa
     5281 agcgatgtta atccagccat taatccgcaa cgccagagga ttattttttg ctgccagcaa
     5341 agcgtaatgc gccatcagca ttgccagagg agcaaagcaa gaaagaatat aggtgggcaa
     5401 tttgcctttg gcgacggaga aaaacaacag cggcattatc gtccagctca acaaatagac
     5461 ggttgcggaa tgcttgcggt ttttccagcc tgcgtacagt gctccgggga gtaatcccag
     5521 ccacggcagg ctaccggcaa tgatgaccgg caagtagtac cagaacggag ctctatgttg
     5581 ggcatcatcc attgcaaagc gttgaatatg ctcaacccag aaaaaatagt gccagaagtc
     5641 aggctcccgc tgcgctatcg ccagtcccca ggggagaacc gtcagtacgc aactgatgac
     5701 cgccagccag ccgtaaataa agagatcttt ccagcgtttt tgcgttgcaa cccatggaag
     5761 cacgcttaat accggcacgg caagggcgag aaaacctttg gtcatcaccc ccatgccgca
     5821 agtgattccc agcagtaaaa atcccgcgct tttgcctttc cacgtctgtg cctgcattgc
     5881 cagccagaag ctgcacattc ccgccaccag ccagaaggca ataaacggat cgagcacggc
     5941 ataagtgcca atggcataga caataaacaa tgagagataa atcactgcgg cgagtagagc
     6001 cagacgttta tcgcgccata agcgcagagt aaaccaggtc accagcgcgg cagttaacag
     6061 ggtcgcaaag ataacgcctg cccgcacacc aaagttattc gcgccaaata gccattgccc
     6121 aatgctgtta atccagtatc cggcaatggg tttttcgaaa taacgtagcc ctaacagatg
     6181 gggcacaatc cagtcgccgg atgccagcat ttctcgactg atttccgcat aacgcgtttc
     6241 atcgggttgc catagcagac gcgtgctgat cggtaacagg taatagcagg caataaacgc
     6301 gaagaggccg ataaggtaac gtaccgattt catcagcgac tacccgcaat ttgttggcaa
     6361 cccagccagc cttcacgtcc ggcaatattt ccgcgaacaa cttgtccgag cggcaacatc
     6421 cctgataaca gttcgcttaa agggcaaaat gtcacgcctt cctgggctgc ccgtttaagg
     6481 agatccacaa aattatgctg ataagcgcag ccttcaactt ctgcatgaat ggtataaacc
     6541 ggcgtgcctt tatctcgcag gatgcgattg agtaaccaac cgttaaaatc ttctgctttc
     6601 acatcccgac caatcacttc atcccaggtg ggtaaggtca ccggaatttg cgcagtacca
     6661 gggtttcctg attcgagcag tggacgaaac ggcatcgccc cacgacaatc gctgttgtag
     6721 cgcaaatgga acgcttcttt tgcttcgatc acctgctggt cggcacgcca gccagcggct
     6781 gcggagcagg ttaccggttg gccgataatt tcttccaggg tgcgaaggcc gcgtgcgata
     6841 tcgtcgatca ttgtttgccg atcccaatta ccgctatggg cttgccaggc atggtgatcc
     6901 caggcgtgca ggccgacttc gtgatgttta gccgcttcac gaatgatatc ggcattggca
     6961 tgaccaatct ctttacccgg ccaggccgta cctgccagta aaatatccca gccataaagc
     7021 gatgccgcgt ttgagcgcag catcttccac aaaaactgtg gcttcaccag tcgccagaga
     7081 tggcggccca tattgtccgg gccgacgctg aaaaaaatgc tggcctgaat attatgctta
     7141 ctcaagattt ccagcagacg cggcacgcct tcacgggtgc cacgaaaggt atcgacatca
     7201 atgcgtaagc ctactttggt catgatggtt tatccgtaag atcaacggtg cgcaggaaga
     7261 aatccagcgt ttcgtcgatg gtttcctgca tatcaatttt cggttcccag tccaggcagc
     7321 ggcgggcatt gcggatgctc ggtttacgat gctctacgtc ctgatatcct ttgccatagt
     7381 agctgctact ttcgacaaca cgaaagcctg caaacggcgg gaagtgatgg cgcagcggat
     7441 gtttttcgaa gctcgccagc agcatctcgc ccagttcctc aatgctcgct tcgttctcag
     7501 gattgccaat gttgataatt tcgccgtcgc agcgatttcc cgcattttcg ataatgcggt
     7561 acaacgcctc gataccatcc cggatatcag taaagcagcg tttttgtttt ccgccatcaa
     7621 ttagcttaat tggtgaacct tctaccagat tgagaatgag ttgcgtaata gcgcgggagc
     7681 tgccaattcg cgctgcatta aggttatcca gtcgtggtcc catccagtta aacggccgga
     7741 agagggtgaa ctgtaaaccc tctttttcgc cataggccca gatcacccga tcaagtaatt
     7801 gttttgacac cgaataaatc cagcgtggtt tattcaccgg gccgacggtt aaattagaat
     7861 ggtcctcatc gaagtattta tcgctacaca tcccataaac ttctgaagtt gacgggaaga
     7921 tgattcgctt acggtacttc acgcagtagc ggataatgcg cagattctct tcaaaatcga
     7981 gttcaaatac gcgcagcggg ttgcgggtat attcaatcgg cgtggctatc gccaccagcg
     8041 gcaagacgac atcacatttt ttgacgtgat actcaatcca ttcggaatga atactgatat
     8101 cgccttcgac aaagtgaaaa tgcggatgat tcagaaaacg gcttatcgca tcgctgccaa
     8161 tatccagacc gtaaacttca taatgatctt cgcgcagcag gcgttctgtc agatggttgc
     8221 caataaagcc attcaccccg aggatgagta cccgggtacg gcgtcgggcg gcacaggcag
     8281 gctggctatt caagcgtgaa ccttgcacca ggcccagcgt ctgcgctaat tgcgagccct
     8341 gcatagtaat gccgtcgccc gcctgtccgg tgacgatttc cagcgcgcca tcgccacagg
     8401 caatcagcag tggcgctaca gaaatcacgc tccccggctg tgctttgctg gcatgaggat
     8461 gaacacgcga cgaccagacg gtgaatttct gattgccaac atagctgaag gcacccggcc
     8521 acggatcggc aacggcacgt accatgttgt gcagtacgga tgccggctta tgccattcga
     8581 ggaaactgtc atccggcgtt ctgcgaccaa aacaggtagc ttcgttttcg cgctgggcga
     8641 tttccagaat attaccgtgt ttaatggtgg gtaatgtctg ctccagtagc tggcgcgcgg
     8701 catgacataa cttatgatgc aacgtaatgg cgatatcgtc tggcgcaatg gcaatgcgca
     8761 gttgcgccac aatggccccg gcatcggcac gtttcaccat tcggtgcaat gtaacgccag
     8821 tttccgtttc accgttgacc agcacccagt tcagcggcgc gcgaccacga tattttggta
     8881 acagcgaacc atgcagatta aatgcacctg cgggagcgag ctgcaaaatc tcgtcgcaaa
     8941 taagatggcg ataataaaaa gagaaaatca cctctggtga gagttgggca atacgttcca
     9001 cccacagcgg atgattaacg ttatctggcg cataaaccgg aatgcctctt tccgccgcca
     9061 gacgagctac cgaaccataa aaggcttttt caccgggatt atcggtatga gtaaaaatag
     9121 cgctaatttc gtaaccggca gccagcaggg cttcaatacc gaggcatccc atatcgtggt
     9181 aggcaaaaac gacggttttc attattcatt ttccttgctg gatggacgga taacttgctg
     9241 aacaaaatag cgggggcggg cgcggacatc ggtgtagatc ctgccgatat attcaccgag
     9301 taatcccatg ccgataaact gagcgccaat aaaagtaaac agcacggcaa atagcataaa
     9361 gacgccttct gccgcccatt gtggtccgaa ggtcaggcgt aaaatcacca gcagcaccgc
     9421 aatgctaaaa cctccaatcg caataatgct gccgagcaga ctcagcatac gtagcggcgt
     9481 tgtggtaagg caggtcacca ggtcgtacat caaattaatc aggcgcataa aactgtattt
     9541 ggattcacca aactcacgct cggcatggtg cactggaatt tcaatggcac ggcgggcgaa
     9601 gatattcgcc agaatcggga taaaggtact gcgttcatgg cagtgcaaca tcgcatcgac
     9661 aatatgacgg cgataggcgc gcagcataca accgtagtca cccatcgctt tgccggtggt
     9721 gcgctgaata agccggttaa tcatcttcga agcggtttta cgaaaccagc tgtcctggcg
     9781 gttctggcgt acagtcccta ccacgtcgta accttcatcg gcttttgcca ccagacgggg
     9841 gatttcctct ggcggattct ggagatcggc atcgagggta ataattaagt cgccagtaac
     9901 gtgactgaat cccgccataa tcgctgaatg ttgcccgtaa ttgcggttaa gcaaaataga
     9961 cacaatatgg ctgttctccg cttgtgaggc ttcgaccagc atatgcgcgg aattatcgct
    10021 actgccgtca tcaatcagca ggatttcata ctctttcccc agtgattcac aggctgcggt
    10081 ggtgcgcctg attaattccg gtaagctttc ctgctcgtta taaacgggaa taaccaccga
    10141 gactttctta acagggtgga tttcaaacat ggcttattgt cctgcgagtt gctgaagggc
    10201 tgtgatgacg cggtcggcat cggcggtggt catatccggg aacagcggca acgaacagat
    10261 gcgttcgcta ttccattcgg tattcggtaa cgacagcgtg ggaaaacgct cgcgataata
    10321 tttttgtgtg tgagcggcgc ggaaatgtaa cccggtacca atgcctcttt cttttaacgc
    10381 ttccatcaac gcatcgcgac tgataccaca acgttgttca tcgacacgaa taataaacag
    10441 atgccaggcg tgaacgtgtg accaggcggg aaggcttaat ggctgaaagg ggagagctgc
    10501 cagtgcttgc tgatattgct gggcaatttc gcgccgacgg gtgttgaggt gctctaattt
    10561 gactaactgt gtcagggcaa tcgcggcgtt aatatcggtc agattgtact tatagcccgg
    10621 cgttaagact tcagcctgcg gtgcacggcc ccaggtatgt ctgtcatagg catcgacgcc
    10681 cagaccgtga aattttagca tccgtagctg gcgggcaagg ttttcattat ctgtaacaat
    10741 caggccacct tccgcacagg taatattttt gatggcgtga aacgaaaaaa tagcggtact
    10801 ttttgcgcca atatgtcgcc ctttgtaata cgtacctacg gcatgggcag catcttcgat
    10861 aactgcgatg ccgtaacgtt cgccaatggc gcgaatggcg tcaatatctg ctggcgcacc
    10921 ggcataatgc accggaatga tggctttagt gcgtggcgta atggctgact cgatcgcttc
    10981 aggcgtgacc atcagcgtat cgcggtcgac atccaccatt accggcgttg cacccaacaa
    11041 ggagatcata ttgagggttg aaacccaggt cagggaaggc gtaatcactt catcgccctt
    11101 gccaatttcc aacgccatta gcgtgatatg cattccggcg gtggctgaac tgaccgcgat
    11161 ggcatgctga tttcccgtca actggcaaaa ggcttgttca agcgcctgat tcttcggacc
    11221 ggttgtgatc caaccggatt cgagaacctc tttaactgct gcgagttcct ccacgcccat
    11281 tgctggtcgc gaaaaaggca aaaattctga cattgctttt ccttccgcca ttgaatacct
    11341 gtccacttat atttgctata aagagtgttg tgtatatttt gccatttgga gcgaaattct
    11401 aaggatagaa tattaactta accttaagaa actaatatta gacgtaaata ttgaaatttt
    11461 tatatttttt cttatttagg ctttgcattt ggcaaaattt tgaggcattt tgccggcatc
    11521 gtaggatttt taatattaca ccaactgcta attatcgcca gaaatgtcgc tcaatttcac
    11581 ggtaattgtc tggttgcgct tgtctatagg tggagtttac gtgttagctt tttgccgctc
    11641 ttcgttgaag tcaaaaaaat atttcatcat tttactggcg ctcgctgcaa ttgccggact
    11701 gggtactcat gccgcctgga gtagcaatgg tttgccacgt atcgacaata aaacgctggc
    11761 cagactggca cagcagcatc cggttgtcgt tttgtttcgc catgctgaac gttgcgatcg
    11821 ttcaaccaat caatgcttgt cagataaaac aggtattacg gtcaaaggta cccaggatgc
    11881 ccgtgaactg ggcaacgctt ttagtgctga tatccctgat ttcgatctct attccagtaa
    11941 taccgtccgg accattcagt cggcgacctg gttttcagcg ggtaaaaagc tgacggtaga
    12001 taaacgactt cttcaatgcg gtaatgagat ttatagtgca attaaagact tacaacgcaa
    12061 agcgcctgat aaaaatatcg ttatttttac ccataatcat tgcctgacat atattgctaa
    12121 agataagcgt gatgcgacat ttaaacctga ttatctggat ggtttagtca tgcatgtgga
    12181 aaaaggcaaa gtttatctgg atggggaatt cgttaaccac taaattttca atctgacagc
    12241 cagtgatggc tgtcatcatt attacagaag acctttcaaa cgtaacgttt ttcgggtggc
    12301 gacattcaaa tcatagtgcg ccagatcttc aggttttacc cacgcgtagt cttgaaactc
    12361 ttcgtttatt ttcacttctc ggttggcaga aacgcagtca aaaatcaggt aaatcatata
    12421 aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc tgaaggtcca
    12481 cggcgtgatt tctgtcaaaa gcagctgttc tcccagctct tcgcgaattt cgcggcgtag
    12541 tgcctcttca attcgttcgc cgggctccac gccgccaccc gaaagcgccc attgaccggg
    12601 gaaaacgccg cgatcgtcgg ccattttaca cagcaaataa gcaccatcat tttgaatcaa
    12661 agggcataca atagtccgtt gtcgcacagg ggtattcctt ataaataaaa agaaaccatg
    12721 agaatacggt aactcagaaa tatgtgaaag tgacagtaat ctcttttgcg atgataactg
    12781 cacttattat tatttttgtg tgtatcggta aactgtgttt attttttcgg cgtttaatta
    12841 tttaagaagt atttagttac aataagtgac tagatccctt ttttcgctca ttgaatcctc
    12901 tgcgccaggc agtacaatcc cgcagctttg ggaattttga ttaattgctt gtgggaaagg
    12961 ggataatgaa aaaaattgcg ctagcaggtc tggccggaat gctgttggtt tctgcatcgg
    13021 tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata caccaatatt ggtgtcggtt
    13081 ttgggactga atcgacgggc ctggcgttaa gcggtaactg gacacataac gacgacgacg
    13141 gtgacgtcgc gggcgtgggg ctggggttga atctgcctct cgggccgtta atggcgaccg
    13201 ttggcggaaa aggcgtttac accaacccga attacggcga tgaaggttat gccgcagcgg
    13261 taggaggtgg tttgcagtgg aaaattggca acagcttccg cttgtttggc gagtattact
    13321 actctccgga ttcgctctcc agcggtattc aaagttatga ggaagcgaat gctggcgcgc
    13381 gttacaccat tatgcgtcca gtcagtattg aggcgggtta tcgctacctg aacctttctg
    13441 gtaaagacgg taaccgcgac aacgcagtgg ctgacggccc gtatgtgggg gttaacgcca
    13501 gtttttgatc ccttccggcg cggcattctg ccgcgccatt cagtttctta ctacgcctta
    13561 tctccctttg cgttatagtg attcgataac aaaagcagga gataaccatg ttaaaagtgg
    13621 aaatgttatc caccggggat gaagtgttac acgggcaaat cgttgacact aacgctgcct
    13681 ggctggccga ttttttcttt catcaggggt tgcctttaac tcgccgcaat acggtggggg
    13741 ataaccttga tgacttagtg accattcttc gcgaacggag tcagcacgcc gatgtgctga
    13801 tcgttaacgg cgggctgggg ccgaccagtg atgatttaag cgcactcgcc gctgcgacag
    13861 caaaaggtga aggcctggtg ctgcatgagg cctggctcaa agagatggaa cgctatttcc
    13921 acgaacgtgg acgagtaatg gcaccgagca accgtaaaca agcggagctg cctgccagtg
    13981 ctgaatttat caataacccg gtaggcaccg cctgtggttt tgccgtgcag cttaatcgtt
    14041 gcctgatgtt ctttaccccc ggcgtaccgt cagaatttaa agtgatggtc gagcacgaaa
    14101 tcctgccgcg cctgcgcgag cgtttttctt taccgcagcc gccggtttgt ctgcgtttga
    14161 ctacatttgg tcgttcggaa agcgatctgg cacaaagcct ggacaccctg caactgccgc
    14221 cgggcgtaac aatgggctat cgctcttcta tgccgattat cgaactgaaa ctcaccggac
    14281 cggcaagcga gaaacaggcg atggaaaaac tgtggctgga cgtcaaacgt gttgccggac
    14341 agagcgtgat tttcgaaggt accgaaggac tgccctcgca gatcagccgc gaattgcaaa
    14401 cccgccagtt cagcctgacg ttgagcgagc aattcacagg tggtttattg gctttgcaac
    14461 tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt tccttcacag gaggaaaccc
    14521 tggcgcaaac tgcgcactgg attacagaac ggcgggccaa ccattttgcc gggctggcac
    14581 tggctgtttc gggtttcgag aacgagcatc tcaactttgc gctagccacg ccagatggca
    14641 ctttcgctct gcgtgtgcgt ttcagcacta cacgctacag cctggctatc cgtcaggaag
    14701 tgtgcgcaat gatggcactg aatatgctgc gccgttggtt aaacggccag gacatcgcca
    14761 gtgagcatgg ctggattgag gttgttgagt ccatgacctt atctgtctga ttccgccact
    14821 ctgtgccggt ttactgcacc ggcacattcc ccctccggca aaatttgtcc aacctgttgt
    14881 actcaataag aaaaaatccg tgcgctaatt cacaaatttg tgcgtaattt ctccagtccg
    14941 ctggacgagg agcgggattt gatgagcttc acaattttac tccacctgag caacgagact
    15001 gattccagta tctaagactg gagtccagta tgttggaatc aagcaaagtt cccgccctga
    15061 cacgggctat tgatattctc aatttgattg cccgcattgg cccctgtagt gctgcgacga
    15121 tcattgacac attggggatc cctaaaagca cggcctattt gctgcttaat gaactcaggc
    15181 gtcagcgttt tcttagtctt gatcaccagg aaaatttttg tttgtggacc aggctggtgg
    15241 agctttccgg tcatgcgtta agcaagatgg atctccggga actggctcga ccgcgtttga
    15301 cgcaactgat ggataccacc gggctgttat gtcacctggg gattattgat aacggcagcg
    15361 cttattacat tttgaaagtg gagtcatcag ccactatcag cgtgcgttcc catgaaggaa
    15421 aaagcctttc gctttatcgc tccggaatag gtaaatgcct gcttgcctgg caacctgcag
    15481 ctgtacagca aagtattatc gaagggctga tctgggagca ggcgacaccc acaacgatta
    15541 ctcatccaca gcaacttcac gaagaactgg cgcgtattcg ccgtcagggc tggagctatg
    15601 acaacggcga agattacgcc gatgtccgtt gtgtcgctgc gccggtgttt aacgccaata
    15661 acgagcttac cgctgcgatc tcggtggtcg gtacccgctt acaaatcaat gaagaatacc
    15721 gtgattatct ggcgggcaag gccattgcct gcgccaggga tatttcacgt ctgttgggat
    15781 ggaaaagtcc ctttgactta caggcctcat aacaatggag aacagcatga ccctaccaaa
    15841 aatcaaacag gttcgcgcct ggtttactgg cggtgcgaca gcagaaaaag gcgctggcgg
    15901 cggtgattat cacgaccagg gggcaaatca ctggattgat gatcatattg ccaccccgat
    15961 gagtaaatac cgcgattacg agcagtcacg ccagtcattt ggcattaacg ttcttggcac
    16021 gttgattgtt gaagtcgaag cagaaaacgg ccagaccgga ttcgccgttt cgacagccgg
    16081 tgaaatgggc tgttttattg tcgaaaaaca ccttaaccgt ttcattgagg gtaaatgtgt
    16141 cagtgatatc aaactgatcc acgatcaaat gctcaatgcc accctgtatt actccggctc
    16201 tggtggcctg gtgatgaaca cgatttcctg tgtcgatctg gctctgtggg atctgttcgg
    16261 caaagtggtc gggcttccgg tttataaact tttaggcggc gcagttcgtg atgagattca
    16321 gttctacgcc acaggtgcgc gtccggatct ggcaaaagag atgggcttta tcggtggcaa
    16381 aatgccgacg cactgggggc cacatgatgg cgatgcgggg atccgcaaag atgccgctat
    16441 ggtcgcggat atgcgtgaaa aatgcggtga ggatttctgg ttaatgctcg actgctggat
    16501 gagtcaggac gtgaactatg ccaccaaact ggcccacgct tgcgcgccct ataacctgaa
    16561 atggatcgaa gagtgcctgc cgccacagca gtatgaaggt tatcgcgaac tgaaacgcaa
    16621 cgcgccagtc ggaatgatgg tcaccagcgg tgagcatcac ggcacactgc aatctttccg
    16681 tacgctttca gaaaccggta tcgacattat gcagccggat gttggctggt gcggtggctt
    16741 aaccacgctg gtggaaattg ccgcaatcgc caaatccaga gggcaactgg tggtgccgca
    16801 cggttcgtct gtttactctc accatgcggt gatcagcttc accaatacgc cattcagcga
    16861 attcctgatg accagcccgg attgttcaac gatgcgtccg caatttgatc cgattctgct
    16921 taatgagcca gtgccggtga atggtcgtat tcataaatca gtgcttgata aacccggttt
    16981 cggcgtcgaa ctcaatcgtg actgcaattt gaaacgcccc tacaaccact aatcactccg
    17041 ggcgttgctg tgacaacggt aacgcccaca tattctaagg agctttttat gagcaccgct
    17101 ttgcttgacg ccgtggtgaa gaaaaaccgt gcgcgtttaa ttccgtttat gttggcgctg
    17161 tatgtgctgg cgtttctcga ccgttcgaat atcggttttg ccaaacagac ctaccagatt
    17221 gataccgggc tgagtaatga agcttatgcg ctgggagcag gcattttctt tgtggtatat
    17281 gcgtttctag gtgttccggc gaatcttttg atgcgcaaac tgggggccag aacctggatt
    17341 ggtacgacaa cactgctgtg gggatttctt tcggcagcca tggcatgggc cgatactgaa
    17401 gcgaaatttc tgatcgttcg cactctgctt ggtgctgcgg aggccgggtt cttccctggt
    17461 atgatttatc tcacctcgca atggtttccg cagcgtaatc gcgccagcat tatggggctg
    17521 ttctatatgg gcgcaccgtt ggcgttaaca ctgggatcac cgctttccgg cgcgctgctg
    17581 gagatgcatg gatttatggg acatcccggc tggttctgga tgtttgtgat tgaaggattg
    17641 ctggcagtcg gcgctggggt attcacattc ttttggcttg atgacacacc ggagcaggca
    17701 cgttttctga gtaaagaaga aaaaacattg cttatcaatc aactggcaag tgaagaacaa
    17761 cagaaagtga cttctcgact gagcgatgcg ctgcgtaatg ggcgagtctg gcaactggcg
    17821 attatctacc tgaccattca ggtggcggtt tacggattaa ttttcttcct gccgacccag
    17881 gttgctgcat tgctggggac aaaagtgggc tttacagcgt cggtggtcac cgccattccg
    17941 tgggttgcgg ccttgtttgg gacctggctt attccgcgct attccgataa aaccggcgaa
    18001 cggcgtaatg tcgcagcgct gacattactg gcggcaggca ttggtattgg tctctccggg
    18061 ctgctttctc cagtactggc gatcgtagcg ctgtgtgttg cagccatcgg gtttattgcc
    18121 gtgcagccgg tgttctggac gatgccgaca caactgcttt ccggtacggc gctggcggcg
    18181 gggattggtt ttgtaaacct gtttggtgca gtgggtgggt ttattgcccc gatcctgcgc
    18241 gtgaaagcag aaacgttgtt tgccagcgat gcggcgggat tactgacgct ggcagcggtg
    18301 gcggtcatcg gttcgctgat tattttcact ctgcgtgtaa atcgcactgt tgcgcagacc
    18361 gacgtggcac atcattaata ggttaaggaa cacgatgaac gcattattaa ccaatccctt
    18421 taaagaacgt ttacgcaagg gcgaagtgca aattgggctg tggttaagct caacgactgc
    18481 ctatatggca gaaattgccg ctacttccgg ttatgactgg ttgctgattg acggggagca
    18541 cgcgccaaac accattcagg atctttatca ccagctacag gcggtagcgc cctatgccag
    18601 ccaacccgtg atccgtccgg tggaaggcag taagtcgctg attaaacaag tcctggatat
    18661 tggcgcgcaa actctactga tcccgatggt cgatactgcc gatcaggcac gtcaggtggt
    18721 gtctgccacg cgctatcctc cctacggtga gcgtggtgtc ggggccagtg tggcacgggc
    18781 tgctcgctgg ggacgcattg agaattacat ggcgcacgtc aacgattcgc tttgtctgtt
    18841 ggtgcaggtg gaaagtaaaa cggcactgga taacctggac gaaatcctcg acgtcgaagg
    18901 gattgatggc gtgtttattg gacctgcgga tctttctgcg tcgttgggct acccggataa
    18961 cgccgggcac ccggaagtgc agcgaattat tgaaaccagc attcggcgga tccgtgctgc
    19021 ggataaagcg gctggttttc tggctgtggc tcctgatatg gcgcagcaat gcctggcgtg
    19081 gggagcgaac tttgtcgctg ttggcgttga cacgatgctc tacagcgatg ccctggatca
    19141 acgactggcg atgtttaaat caggcaaaaa tgggccacgc ataaaaggta attattgata
    19201 tcaaaggccc atgggggtcg gctgtgggcc tgtgttaatt agtggttatt cgctgccaga
    19261 tcggcttcgc ttagctgggt ggctgcgagc accaggtcac gatcaatgcc ctgtaccagc
    19321 atgcgcaaag caattttaat ggcttgttta cgcatacctt cctgcaaacc ttctaattta
    19381 ccctcctgcc agccttcaag cttaccttcc tgccagccaa tctgatgccc ttcctgcctc
    19441 agccgttcag caatagtcat caatctctcc ttttaatgac agttcggaat atcagcatca
    19501 gtgagttggg ttgtcgccag cacaatctca cgttcaaagc cctgctccag catgcgcaag
    19561 gcaattttaa tggcttgttc atgcatgcct tcctgcaaac catctaattt accttcttgc
    19621 aaaccttcta atttaccttc ctgccaacct tcaattttac cttcctgcca gccttcaatc
    19681 ttaccttcct gccagccaat ttgatgccct tcctgccgca gccgttcagc aatagtcatt
    19741 aatctctcct tttgtagtgg tgaacgttcg gcaatctcct caataaaacg ggtgaaacgg
    19801 gaggtatcgc cgcattgcag cagataatta aacagcgttt gtagctggct gtcattagtg
    19861 aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatac cgattaaatc gcggtcgcga
    19921 atatgctttt gaatcagttc aagcagagct atacgccgat gttgcatgat ctcatcgtca
    19981 ggtacgatgg tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc
    20041 ggatcgtcaa actcatccag ccagttgagt gagtacgggt agggcgaggt ttcgccatga
    20101 taaaacagca atggcaccac cagcggaact ctgtcatagc ctttatccag gtgacgctgc
    20161 atggcggcag tggcatagcg cattagccga aaagccatat tcttttctgc agagctttga
    20221 tgttcaatca cgcagtagat atagccttca ctgtcgctgg tttccacaga ccacaaaaca
    20281 tccgagtagt aagcgcgtaa acttttttca ataaagctgg tgggttccag gcgtaaagtt
    20341 tgcaggttgc aaagcttgcg cagcggctct ggtagatgta tttccagaaa atcccgagcg
    20401 gtttcgggtg tgaacataaa ggttttaaac accgcatcat gcggggagga ggttgttgat
    20461 tcggtcatgg ttgtccgtca cctgatattt atgatgacgc gacattacgg ttatcccagg
    20521 cctttgacat ctgccagttt cagcctttgc gagacgtttt cagaggaatt ttcactgcgt
    20581 tgcaacgcat acattgggca cggaatcgtg tacgcaaaaa tgcccggtaa caacatgctt
    20641 accgggcatc agaaaggagt ttaagccagc gcctgggcca gcagcgtaat cggatgttcg
    20701 cagcgaagac ttgtggacat ctcaatctgc catttgcagg tttcgcagtc ggtaaccacc
    20761 agatctgcgc cgctttcttc tatctgacgg aacagtggtg cgccgatggc ttgtgaggtg
    20821 gggtagttct cttttttgaa accgtaagta cctgcaatac cgcagcactg ggaatccagc
    20881 accgttaact caagccccgg gattttacgc aacagctcca gggtgtagag cgtccagccc
    20941 attttttcca tatggcacgg agtgtgataa accactttca gcggcagcgg tttcagcggc
    21001 aaggttttgc cttcgtccag cttgcgccac agccagcggg tcgccagttc gatgtgatcg
    21061 cgcaagcctt tgttgtcgac attcagcact tccggatatt cgtcgcgcag ggcaaatgta
    21121 caggttgagg aggtggcaat caccggaatg ccttttactc ccactgcttc gcggatcgac
    21181 tctacatttg tgattgcctg tttacgtgct ttatcggtaa agccgttggc gatcagcggt
    21241 acgccgcagc atttttcttt gctgagcagt tgtacaccgg tacccattgc gttgagcact
    21301 ttaattaaat ctttgcctaa ctgcggatgg ttgtagttaa cgaagcagcc gtgaaagaaa
    21361 gccacctggt ctttatattg tgcctgctgg gccgccacgc tgcgatacca gcgacggaac
    21421 gtaccgaagg agtatttcgg tagcgtgcgg cgatgatcga tttttaacgc cgcatcaagc
    21481 agctgccgca ctggtttcag cgaggtagcg gtattgacga ttggtgcgaa tggcgtggaa
    21541 acgctaccca tcaggtcggt atgactcaac acaaaattgc gcagcgacgg gcgtgtggtg
    21601 tcatatttcg cccgcgcgcg ctggatgata tcgccaatct tcacatcgga cgggcaggcg
    21661 acttcgcagc gtttgcagtt gatgcaatat ttcagcgctt cgtcatacag tgcgccatct
    21721 ttcagacgca gacgctcgcc atccggcccg gcttgttttg gccctggata gccgggattc
    21781 acccggctca ccgggcaggc ggtggtgcag acggtgcact taatgcagtt ttcgaagctg
    21841 gtgtcgttca tagttggcct cctgcgcgtt gggcaatctg ttgagcggca tgtaaagcac
    21901 tgacggcaca aacaccgccg ccgcatccct gggcgatggg atcaaatccg cccagcaccg
    21961 aaccgatggc aaacaggttc tcaatggttt gccctgcctg cgatgggcgt agtgcctcat
    22021 cagtggttac accgaacagc tgccacggtt gcggcgcaaa aaaatcgccc ttataccatt
    22081 caccccgcgt ggcggtttgt agcacatcaa ggccgagaat cggctcgcga atgccgtcac
    22141 gttctgccac cagtccgccg ctaaagaaac tgccgctcgc aagcaccgca aatcgtgggc
    22201 gcagcgggat atcggcgtga ttgcgcgtcc agatttcatt cactacgcca tttttacagg
    22261 tcactttttt cacttcatcg cctggcatcc acacgccacc ctggcgcaca aactggcgct
    22321 gtaactggtt ttgcagacga atgcccagca cggaaggcgg cagcgttggc aaaagcatca
    22381 gtgaacaagg tagtttttca ttcaaccaac gccacagttt gtcatcggcc aggccgaagc
    22441 aggcgggcat caggatcatt tcgcaggtat tggcgacagg aataagcgca tcaagtaaca
    22501 gcgaccagtt ctcttcgtta tcaaggaaac gggcgatatt caccgcgcga aactcggtgg
    22561 cgttgttgcg cagcacatcc agttccggca gctctatttc tgcggtttca acggcaaggt
    22621 cgagttcgcg caacgaagcc gccgcaagat gcgcctgaaa atccatcagg ccgcttatcc
    22681 cgaccacaca tattttcttc gcgggcaacg gccagacagg aacttctggc gaacttagcc
    22741 aggttgagcg cagagtgccg agcggggtaa ctcgctggtg ggccagttct acgctgccct
    22801 gtaattgcgc accgctttca gcgattaatg cctgcgcctg gcaagcgaga tcgagtacgc
    22861 gttgtggccc gagaagagtg taaggatggg caggtgcctg ctgacgcaaa gcttccagtc
    22921 cactgtgaat gtctgtcacc ggttgaccat ccggcagatg gctcagcaaa tccagcgatc
    22981 cagatgagaa atggagcgcg ctttgaccac gcgtgacaat ggcacagcgc aggccgtgtt
    23041 tttgcagttg caggccacag agtaatccgg cgaggccgcc gcccataatg acagtatcaa
    23101 agcgcatctt tctgctcctt ctccagacca cacagtccct gataaaccca gcgggtaaat
    23161 tcgctttcgc gcagtgcatc tccccaggcg atgggttgca cgcctttcca gcgttcgtta
    23221 aggaaggtgg aaagttgctc gatagattgc gcggacatcg tgacgttaaa acgttgcagc
    23281 agtccggcag cgcggcaggc gcagagttcg ccctggcagg tacccatccc cacacgggta
    23341 cgacggcgta aatccagcag gctattaacg tttaaatttt ctaccgcgta ctgaacttca
    23401 cccgcagtta ccgcttcgca ctcacatacc aggctacggt gcagacggcc ttcgctcagc
    23461 caggcgggtg tgcgatcgcc atgacgataa accgcagaac cgcgcagcgg ggcaggcagg
    23521 gagatgactt tacgcaaggt aacttcagcg ggctcctgtg aaccaggaag tgccagatcg
    23581 gcggttgtac aggggcgcgt gttgcccagc ttgcgacata ccgcgtcggt agcccattcc
    23641 gccatcagcc gataggtcat cagtttgccg ccggtgatgg tgataaatcc gtccagacca
    23701 tcgcgttcag catggtcgag cagcacgatg ccacgactga cgttacggcc gctcgggtcg
    23761 tcatcgctgg caaccagcgg acgcacgcca gagtaagccc gcaaaatacg cgttttcgcc
    23821 atcacggggg ccagtttttc cccttcacgc agcagaatat caacttcttc tgccgtcact
    23881 cgattatcgt caatctcatt gtaatcaata cgtaaagagg tggtgccaat tagcgaaatg
    23941 gtatcgccag gcaccagaat atcggcgtcg gaaggtttgc ggcagcggtt gatcacatgc
    24001 tgattaatgc ggtgatccat gatcagcagc gatcctttcg ccgggaacat gcgaatgcgc
    24061 agatcggcat attcggcaat gcgttgcccc cagatcccag cggcattaac cacgacaggc
    24121 gcatgaaggg cctgagtttc gccggtgaga tggttacgta cacgaacgcc gcacaccgtc
    24181 gcaccttcac gaatcagccc cgtgacttca tgagcggtaa ggataacggc accgtgttct
    24241 ttggcatcca gcatgtttgc tgcggtcaga cgaaatggat caacggtgcc atccggaact
    24301 ttcaccgcgc caatcagtgc cgggttaacg gcaggttcga taatgcgcgc ttgctgcggg
    24361 tctatagctt ctgcgctgat ccctgcttct tcgcaggcgc ggataaaagt ggcctggaag
    24421 gagaggtcat cttccggcag ggtgataaac aggccgttgg tcggttcaac gcagtgacgt
    24481 gcaatgcgtt tcaggatctg gttttcacta atacattcgc gggccgattc cgcatcggtt
    24541 accgcatagc gcgcaccgct gtgcagcagg ccgtggttac gcccggtggc accggttgcg
    24601 atgtcgtggc gctcaaccaa aatcacgcgc agtccgcgca gggcacagtc gcgggcaatc
    24661 ccggctcccg ttgcgccgcc gccaatgata atcacgtcac ttgattgcga gtcgcgagtt
    24721 ttcattgtta tccctctgaa gttcgttttt taccatttag ccatagtaaa aacatgaatt
    24781 gtttgatttc gcgcatattc gctcataatt cgaaagtgaa acgtgatttc atgcgtcatt
    24841 atgaacattt aacccatcga atttaataat gtgtgcggta attcacattt aatttatgaa
    24901 tgttttctta acatcgcggc aactcaagaa acggcaggtt ctcccactga atcaggctgt
    24961 taatcataaa taagaccacg ggccacggag gctatcaatg ttgagtattt ttaaaccagc
    25021 gccacacaaa gcgcgcttac ctgccgcgga gatcgatccg acttatcgtc gattgcgctg
    25081 gcaaattttc ctggggatat tctttggtta tgctgcttac tatttggttc gtaagaactt
    25141 tgccctcgct atgccttatc tggtcgagca gggattctca cgcggtgatt taggttttgc
    25201 cctttcgggg atctcgattg cttatggatt ttcgaaattc atcatgggct cggtgtcgga
    25261 tcgctcgaat ccgcgcgttt tcctgcccgc aggtttgatt ctggcggcgg cggtgatgct
    25321 ttttatgggc tttgtgccgt gggcgacgtc gagcattgcg gtgatgtttg tgctgctgtt
    25381 cctctgcggt tggttccagg ggatgggatg gccgccgtgc ggtcgtacta tggtgcactg
    25441 gtggtcgcag aaagaacgtg gcggcattgt gtcagtgtgg aactgtgcgc ataacgtcgg
    25501 tggtggtatt ccgccgctgc tgttcctgct ggggatggcc tggttcaatg actggcacgc
    25561 ggcgctctat atgcccgctt tctgcgccat tctggtggca ctgttcgcct ttgcgatgat
    25621 gcgcgatacc ccgcaatcct gtggcctgcc gccgatcgaa gagtacaaaa atgattatcc
    25681 ggacgactat aacgaaaaag cggaacagga gctgacggcg aagcaaatct tcatgcagta
    25741 cgtgctgccg aacaaactgc tgtggtacat cgctatcgcc aacgtgttcg tttatctgct
    25801 gcgttacggc atcctcgact ggtcaccgac ttatctgaaa gaggtgaaac acttcgcgct
    25861 ggataaatcc tcctgggcct acttcttcta tgagtatgcg ggcattccgg gcaccctgct
    25921 gtgcggctgg atgtcggata aagtcttccg tggcaaccgt ggggccaccg gcgtcttctt
    25981 tatgacactg gtgaccatcg cgactatcgt ttactggatg aacccggcag gtaacccaac
    26041 cgtagatatg atttgtatga ttgttatcgg cttcctgatc tacggccctg tgatgctgat
    26101 cggtctgcat gcgctggaac tggcaccgaa aaaagcggca ggtacggcag cgggctttac
    26161 cgggctgttt ggttacctgg gcggttcggt ggcggcgagc gcgattgttg gctacacagt
    26221 ggacttcttc ggctgggatg gcggctttat ggtaatgatt ggcggcagca ttctggcggt
    26281 tatcttgttg attgttgtga tgattggcga aaaacgtcgc catcaggaat tactgcaaaa
    26341 acgcaacgga ggctaatggc atgaaattga cgctgaaaaa ccttagcatg gcgatcatga
    26401 tgagtggaat gatcatggga agcagtgcaa tggcggcgga cagcaacgaa aaaatagtca
    26461 tcgcccatcg cggtgccagt ggatatttgc cggagcatac gctgccagca aaagcgatgg
    26521 cgtatgcgca gggagcggat tatctggaac aagatttggt gatgaccaaa gacgaccatc
    26581 tggttgtcct gcatgaccat tatctcgatc gtgttactga tgttgccgat cgtttcccgg
    26641 atcgggcgcg caaagacggt cgttactacg cgatagattt cacgctcgat gaaattaagt
    26701 cactgaaatt taccgaaggt ttcgatattg aaaatggtaa gaaagtacag acttatccgg
    26761 ggcgtttccc gatgggtaaa tctgacttcc gggtacacac tttcgaggaa gagattgaat
    26821 ttgttcaggg gttaaatcac tctaccggga aaaatatcgg tatctatcca gaaattaaag
    26881 cgccgtggtt ccatcatcag gaagggaagg atattgcggc aaaaacgctg gaagtgctga
    26941 agaaatatgg ttacagcggt aaggacgaca aagtttattt gcaatgtttt gatgccgatg
    27001 aactgaagcg tattaagaat gagctggaac ctaaaatggg catggacctc aatctggtac
    27061 agctgattgc ctataccgac tggaacgaaa ctcagcagaa acagccggac ggaagctggg
    27121 ttaattacaa ctacgactgg atgtttaagc cgggggccat gaaacaggtg gcggaatatg
    27181 ctgatggtat tggtccggat tatcatatgt tgattgagga gacatcgcag ccaggtaata
    27241 tcaaactcac tggcatggtg caagatgctc agcagaacaa gctggtagtg catccttata
    27301 ccgtgcggtc agataaactg cctgaataca ctactgatgt gaatcagtta tatgatgctc
    27361 tgtataacaa agcgggtgta aatgggctat ttactgattt ccctgataag gcagtaaaat
    27421 tccttaataa agagtaaatt aactaccgga tggcacttat gtgctatccg ataccactct
    27481 tcattaaagt ctgtgggtta tccttaattg caattatttt gagcgagatg acttgttccc
    27541 ggcataagat actcgtcaat acaacacgta ttattaagat tttcctcagc taaacatcta
    27601 tagtcattgt cgttaataaa aaacgacagg cgatagggta tggcagtttc agcaaagcac
    27661 gacgaattta atcactggtg ggcaacagag ggcgactggg ttgaagagcc taactatcgc
    27721 cgtaatggta tgagcggggt gcaatgcgtc gagcgcaacg gcaaaaagct atatgtaaag
    27781 cgcatgacgc atcatctgtt tcattccgta cgttatccgt tcggccgacc aacgattgtc
    27841 cgtgaggttg cggtaattaa agaactggaa cgggccggtg tcatcgtgcc gaagatcgtt
    27901 tttggtgaag cggtgaaaat tgagggggaa tggcgcgcgt tgctggtgac tgaagatatg
    27961 gcggggttca tcagcattgc tgactggtat gcccagcatg cagtaacgcc ctattctgac
    28021 gaagtacggc aagccatgtt gaaagcagtg gcgctggcgt ttaagaaaat gcatagcgtt
    28081 aatcgtcagc atggctgttg ctatgttcgc catatctatg tgaaaacaga aggtaagtca
    28141 gaagctggtt ttcttgatct ggagaaaagt cgtcgccgct tgcgtcgcga taaagcgatt
    28201 aatcatgact tccgccagct ggaaaaatat ctggagccga tccctaaagc ggactgggag
    28261 caggtgaaag cgtattacta cgccatgtaa tcgttgtagg ccagataata cgcgtaagcg
    28321 tcgtatctgg cgctagtacc cgatcacaac tcgatttcaa tatcgccttt tgcccggcaa
    28381 caacagggca aaatttcccc cggctgaata aaggctaacg gttcggcaat ccagtccacc
    28441 tggcctgcaa caaggcgcgt gcgacaggaa ccgcagtaac cttcgcggca ctggtactca
    28501 accgccacat tgtgggattc cagcgccgcc agaagggaag ggtgttcatc ctggcacagc
    28561 agttgtgtgc cagtgatgcg cagggtaacg cgagccatca gagctggaag ttactcaaat
    28621 cgtcggtgtc cacttccgag tcaatctgcc cgaccagata agaactgact tccacttcct
    28681 gcggagcaac ctgcacgtta tcagacacca gccaggtatt gatccacggg atcgggttgg
    28741 agcgtgtctg gaacggcaga tccaaaccga ctgcctgcat gcggatattg gtgatgtatt
    28801 caacgtactg gcagagaatg tctttattca gaccaatcat cgaaccgtca cggaacagat
    28861 aatccgccca gtctttctct tgctgtgccg cctggacaaa caggtcatag cactcctgct
    28921 tacattcttc ggcaatttcc gccatctccg gatcatccgc gccgctgcgc agcagattca
    28981 gcatatgctg agtaccggtc aggtgcaggg cttcatcgcg agcaatcagg cgaataattt
    29041 tggcgttacc ttccatcagt tcgcgttctg caaaggcaaa ggaacaagca aagctgacgt
    29101 agaagcggat cgcttccagc gcgttaacgc tcatcaggca gagatacagt tttttcttca
    29161 gctcgcgcag gctaacggtt acggttttac cgttaacggt gtgggtacct tcgcccagca
    29221 gatgccagta gctggtcatt tcgatcagct catcgtaata gctggagatc ccttccgcac
    29281 gtttctggat ctgctcgttg gtgacgatat cgtcaaacac aacagacgga tcgttaacga
    29341 tattacgaat gatatgggta taagaacgag agtgaatggt ttctgagaac gcccaggttt
    29401 cgacccaggt ttccagctca gggatcgaga tcagcggcag cagtgccacg ttcgggctgc
    29461 gaccctgaat ggagtccagc agcgtctgat atttcaggtt gctgataaag atgtgttttt
    29521 cgtgttcagg tagtgcctgg taatcgatac ggtcacgaga aacgtcaact tcttccggac
    29581 gccagaagaa agagagctgc ttttcgatca gcttttcgaa gatgtcatat ttttgctgat
    29641 cgtagcgagc cacgttaacc ggctgaccaa agaacatcgg ttctttcagc tgatcatttt
    29701 tcgtctgtga aaaggtggta tatgccatga atgtgtcctg ttgggagttt aatgccggat
    29761 aaggcatttt acgccgcatc cggcatctca atatcagatc ttacatgcgc cgctttcgca
    29821 gccatcgtcc tggattgacg gtaccagatc gtcttgtgcg tcttcagcac cgtcacgggt
    29881 gttctgataa tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcag
    29941 ttgctgcatc ggcacttttc ctgacgggaa gcgtgacgga tcgtagttgg tgttagcaga
    30001 gatcgactga tcgataaatt tctgcatgat acccaccagt tgcaggtagc catcgttacc
    30061 cggcatttcc cacagcagtt cataggcgtc gtgcagatgc tcgtagtccg gcaccacctg
    30121 gcgcagaata ccgtctttcg acgctttgat gctgacgtaa ccacgcggcg gttcaatacc
    30181 gttagtggcg ttagagatct gcgaagaggt ctcggacggc atcagggcag aaagcgtgga
    30241 gttacgcagg ccgtgcgttt tgattgactc acgcagtgct tcccagtcgt aatgcagcgg
    30301 ctcattagcg atggcatcca gatctttctt ataggtatcg atcggcagga tccctttcgc
    30361 gtaagtggtt tcgttaaacc acgggcacgc gccttgctct ttcgccagct cattagacgc
    30421 tttcagcagg taatactgaa tggcttcgaa ggttttatgc gtcaggttgt tggcgctgcc
    30481 gtcggagtag cgtttaccgt gcttcgccag gtagtaagcg aagttgatca caccaatacc
    30541 cagcgtacga cgacccatcg ctccacgttt ggcggccggg atcgggtaat cctgataatc
    30601 cagcagcgcg tcgagtgcgc gtactgccag aattgccagc tcttccagtt catccaggct
    30661 gttaattgcg cccaggttga aagcagacag cgtacacagc gcgatttcac cgttctcgtc
    30721 gttgacgtcg ttcagtggtt tggtcggtag ggcgatttcc aggcacaggt tagactggcg
    30781 cactggtgca atagccggat caaacgggct gtgggtgttg cagtggtcta cgttctgaat
    30841 atagatacga ccggtagacg cacgttcctg catcatcagc gagaacagct caacggcttt
    30901 cacacgctgc ttgcggatgc tgtcgtcttt ctcatatttg gtgtacaggc gttcaaactc
    30961 ttcctgatcg gcgaagaacg cgtcgtacag ccccggtacg tcggacgggc tgaacagggt
    31021 gatatcttca cctttcagca gacgggtata catcagtttg ttgatttgta ccccgtagtc
    31081 catatgacgc acgcggttgc cttcaacccc acggttgttt ttcaacacca gcaggctttc
    31141 cacttccaga tgccacatcg ggtagaacag tgttgccgca ccgccgcgca caccgccctg
    31201 agagcaggat ttcaccgctg tctggaaatg tttgtaaaac ggaatacagc cggtgtggaa
    31261 tgcttcgcca ccgcgaatcg ggctacccaa cgcacgaata cgcccggcgt tgatgccgat
    31321 cccggcacgc tgggaaacgt atttaacaat cgcgctggag gtggcgttga tggaatccag
    31381 gctgtcgccg cactcgatca gtacgcagga gctgaactga cgagtcgggg tacgcacgcc
    31441 ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt
    31501 cacgtattgc aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg caaccagaat
    31561 ataaaggaac tgggcgcttt cgtagatttc gccggtcacg cggttctgta ccagatattt
    31621 gccttccagc tgcttaacgg cagcataaga gaaggtcata tcacggtcgt gatcgataaa
    31681 cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc agcagatgat tatcgtattt
    31741 gcccatctcg accattttca ccacgtggtc gtacagcgca ggcggctcaa actggccgta
    31801 ggctttttta cgcaggtgga agatcgccag gcgcgcggcg agatactgat aatccggcgc
    31861 atcacgggag atcaggtctg cggcagcctt gataatggtt tcgtggatgt cagaggtctt
    31921 gataccgtca taaaactgaa tgtgggagcg cagttcgacc tgggaaatcg aaacgttatg
    31981 cagtccttct gccgcccaat ccagaacgcg atggattttg tcgagattga tgcgctctgt
    32041 gctaccgtcg cgctttgtca ccagcagatt ctgattcatg tatgtcgtac ctgtttttgg
    32101 aattctttcc gcaatagttt catgcagact acctgtagtg agggaagctg cttagaacac
    32161 tatatatagg gggtatgttt gggattcact gcaagatagt gtgaaaatgg ccctcttgca
    32221 agtgcataac tttgtggata actcaggaag gaaaaagtgg ctttcgcgca ccttaggtca
    32281 gacaaggtgt ccgggaaagt caatgggaag agaaaaattt gttaaaaata actgttcggt
    32341 gatttcttga acggcagaat aggttggata aattgatata gatggtttat taatcttttt
    32401 gctaatgatt gcaaaaattg atctaagcac gagtcatcac ataatctgat gtatcagttg
    32461 tggtcaatat ataagcgaat gtaagcgatt gttgatgtcg aaatggttgt tttctatgcg
    32521 gttattgtta aatactaagc caatttgtta cttttgtatt tattataaga atattattta
    32581 ttgctttaat ttagtttaaa tataaccgat ttaccttaa
//
LOCUS       DAZGTJ010000056        31445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000056 DAZGTJ010000000
VERSION     DAZGTJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.056"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..182)
                     /locus_tag="ACHK8E_003102"
     CDS             complement(<1..182)
                     /locus_tag="ACHK8E_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFP0345334.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMV"
     gene            complement(290..2965)
                     /gene="adhE"
                     /locus_tag="ACHK8E_003103"
                     /gene_synonym="adhC"
     CDS             complement(290..2965)
                     /gene="adhE"
                     /locus_tag="ACHK8E_003103"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP0345335.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3358..3411
                     /gene="ychE"
                     /locus_tag="ACHK8E_003104"
     CDS             3358..3411
                     /gene="ychE"
                     /locus_tag="ACHK8E_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP0345336.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            3442..4089
                     /gene="ychE"
                     /locus_tag="ACHK8E_003105"
     CDS             3442..4089
                     /gene="ychE"
                     /locus_tag="ACHK8E_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP0345337.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(4247..4543)
                     /locus_tag="ACHK8E_003106"
     CDS             complement(4247..4543)
                     /locus_tag="ACHK8E_003106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345338.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            4827..6458
                     /gene="oppA"
                     /locus_tag="ACHK8E_003107"
     CDS             4827..6458
                     /gene="oppA"
                     /locus_tag="ACHK8E_003107"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP0345339.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6544..7464
                     /gene="oppB"
                     /locus_tag="ACHK8E_003108"
     CDS             6544..7464
                     /gene="oppB"
                     /locus_tag="ACHK8E_003108"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP0345340.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7479..8387
                     /gene="oppC"
                     /locus_tag="ACHK8E_003109"
     CDS             7479..8387
                     /gene="oppC"
                     /locus_tag="ACHK8E_003109"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP0345341.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8399..9412
                     /gene="oppD"
                     /locus_tag="ACHK8E_003110"
     CDS             8399..9412
                     /gene="oppD"
                     /locus_tag="ACHK8E_003110"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP0345342.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9409..10413
                     /gene="oppF"
                     /locus_tag="ACHK8E_003111"
     CDS             9409..10413
                     /gene="oppF"
                     /locus_tag="ACHK8E_003111"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP0345343.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10466..10795)
                     /gene="yciU"
                     /locus_tag="ACHK8E_003112"
     CDS             complement(10466..10795)
                     /gene="yciU"
                     /locus_tag="ACHK8E_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP0345344.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10830..12290)
                     /gene="cls"
                     /locus_tag="ACHK8E_003113"
     CDS             complement(10830..12290)
                     /gene="cls"
                     /locus_tag="ACHK8E_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP0345345.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12433..12606
                     /gene="yciY"
                     /locus_tag="ACHK8E_003114"
     CDS             12433..12606
                     /gene="yciY"
                     /locus_tag="ACHK8E_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP0345346.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12661..13914)
                     /gene="kch"
                     /locus_tag="ACHK8E_003115"
     CDS             complement(12661..13914)
                     /gene="kch"
                     /locus_tag="ACHK8E_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP0345347.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(14215..14511)
                     /gene="yciI"
                     /locus_tag="ACHK8E_003116"
     CDS             complement(14215..14511)
                     /gene="yciI"
                     /locus_tag="ACHK8E_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP0345348.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14735..15454
                     /gene="tonB"
                     /locus_tag="ACHK8E_003117"
     CDS             14735..15454
                     /gene="tonB"
                     /locus_tag="ACHK8E_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP0345349.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(15494..15892)
                     /gene="yciA"
                     /locus_tag="ACHK8E_003118"
     CDS             complement(15494..15892)
                     /gene="yciA"
                     /locus_tag="ACHK8E_003118"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP0345350.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15997..16536)
                     /gene="yciB"
                     /locus_tag="ACHK8E_003119"
     CDS             complement(15997..16536)
                     /gene="yciB"
                     /locus_tag="ACHK8E_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP0345351.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16566..17309)
                     /gene="yciC"
                     /locus_tag="ACHK8E_003120"
     CDS             complement(16566..17309)
                     /gene="yciC"
                     /locus_tag="ACHK8E_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP0345352.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(17337..17426)
                     /locus_tag="ACHK8E_003121"
                     /pseudo
     CDS             complement(17337..17426)
                     /locus_tag="ACHK8E_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17666..18304
                     /gene="ompW"
                     /locus_tag="ACHK8E_003122"
     CDS             17666..18304
                     /gene="ompW"
                     /locus_tag="ACHK8E_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP0345353.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18363..18869)
                     /gene="yciE"
                     /locus_tag="ACHK8E_003123"
     CDS             complement(18363..18869)
                     /gene="yciE"
                     /locus_tag="ACHK8E_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0345354.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(18915..19415)
                     /gene="yciF"
                     /locus_tag="ACHK8E_003124"
     CDS             complement(18915..19415)
                     /gene="yciF"
                     /locus_tag="ACHK8E_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0345355.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(19501..19680)
                     /gene="yciG"
                     /locus_tag="ACHK8E_003125"
     CDS             complement(19501..19680)
                     /gene="yciG"
                     /locus_tag="ACHK8E_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0345356.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(20061..20867)
                     /gene="trpA"
                     /locus_tag="ACHK8E_003126"
     CDS             complement(20061..20867)
                     /gene="trpA"
                     /locus_tag="ACHK8E_003126"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP0345357.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(20867..22060)
                     /gene="trpB"
                     /locus_tag="ACHK8E_003127"
     CDS             complement(20867..22060)
                     /gene="trpB"
                     /locus_tag="ACHK8E_003127"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP0345358.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(22072..23433)
                     /gene="trpCF"
                     /locus_tag="ACHK8E_003128"
     CDS             complement(22072..23433)
                     /gene="trpCF"
                     /locus_tag="ACHK8E_003128"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP0345359.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(23434..25029)
                     /gene="trpD"
                     /locus_tag="ACHK8E_003129"
     CDS             complement(23434..25029)
                     /gene="trpD"
                     /locus_tag="ACHK8E_003129"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP0345360.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(25029..26591)
                     /gene="trpE"
                     /locus_tag="ACHK8E_003130"
     CDS             complement(25029..26591)
                     /gene="trpE"
                     /locus_tag="ACHK8E_003130"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP0345361.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(26613..26706)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(26683..26727)
                     /gene="trpL"
                     /locus_tag="ACHK8E_003131"
     CDS             complement(26683..26727)
                     /gene="trpL"
                     /locus_tag="ACHK8E_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP0345362.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            26865..27746
                     /gene="rnm"
                     /locus_tag="ACHK8E_003132"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             26865..27746
                     /gene="rnm"
                     /locus_tag="ACHK8E_003132"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP0345363.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            27743..28363
                     /gene="yciO"
                     /locus_tag="ACHK8E_003133"
     CDS             27743..28363
                     /gene="yciO"
                     /locus_tag="ACHK8E_003133"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP0345364.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            28391..30280
                     /gene="yciQ"
                     /locus_tag="ACHK8E_003134"
     CDS             28391..30280
                     /gene="yciQ"
                     /locus_tag="ACHK8E_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP0345365.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            30493..31368
                     /gene="rluB"
                     /locus_tag="ACHK8E_003135"
     CDS             30493..31368
                     /gene="rluB"
                     /locus_tag="ACHK8E_003135"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP0345366.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNNG"
BASE COUNT         7946 a         7557 c         7913 g         8029 t
ORIGIN      
        1 accatcattt cgaggggaag acggcgcttg cgtagcgtta cagtacctga ttcggcaagg
       61 caacgagaga tgagttcggg gtcgaggtaa tcccccagag aagtcagtgg gttacgcaga
      121 gaatcgtaac gggataccag atcaagagcc tgtccaatgt gcataaaaaa atccggaaac
      181 aagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc cttaactgat
      241 cggcattacc ccagaagggg ccgtttatgt tgccagacag cgctactgat taagcggatt
      301 ttttcgcttt tttctcagct ttagccggag cggcttcttt cttcgctgca gtttcgcctt
      361 ctacataatc acgaccgtag taagtatcca gcagaatctg tttcagctcg gagatcagcg
      421 ggtaacgcgg gttagcgccg gtgcactggt catcgaatgc atcttcagac agtttatcca
      481 cgttcgccag gaagtctgct tcctgaacgc cagcttcacg gatagatttc ggaataccca
      541 gttcagcttt cagcgtttcc agccatgcca gcagtttctc gatcttagca gcagtacggt
      601 cgcccggtgc gctcagaccc aggtggtcag caatttcagc ataacgacgg cgagcctgcg
      661 gacggtcata ctggctgaat gcagtctgct tggtcgggtt gtcattcgcg ttgtagcgaa
      721 taacgttaca aatcagcagg gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca
      781 gtttgtgcgc cattgagtga catacaccca ggaaggcgtt cgcaaacgcg atacccgcga
      841 tagttgctgc actgtgaaca cgttcacgcg ctaccggatt tttagatcct tcgtggtagg
      901 acgctggcag atattctttc agcagtttca gtgcctgcag tgcctgacca tcagagaact
      961 cagatgccag tacagaaaca taagcttcca tggcgtgagt tactgcgtcc aggccaccga
     1021 aagcacacag ggacttcggc atgtccataa ccaggttggc atcgacaatc gccatatccg
     1081 gggtcagcgc atagtctgcc agcggatatt tctgaccagt agcgtcgtca gttacaaccg
     1141 caaacggagt gacttcagaa cctgtaccag aagtggtggt gacagcgatc attttcgctt
     1201 tcacgcccat tttcgggaac ttgtagatac gtttacggat atccataaag cgcagcgcca
     1261 gttcttcgaa gtgagtttct ggatgctcgt acatcaccca catgatcttc gcggcatcca
     1321 tcggggaacc accacccagc gcgataatca cgtctggttt gaaggagttt gccagttctg
     1381 cacctttacg aacgatgctc agggtcgggt cagcttctac ttcgaagaag acttcagttt
     1441 caacgcctgc tgctttcagt acggaagtga tctgatcagc ataaccattg ttgaacagga
     1501 agcggtcagt cacgatgagc gcacgtttgt ggccatcagt aatcacttca tccagcgcga
     1561 ttggcagaga gccacggcgg aagtagatag atttcggaag tttgtgccac aacatgtttt
     1621 cagctcgctt agcaacggtt ttcttgttga tcaggtgttt cggaccaacg ttttcagaga
     1681 tggagttacc accccaggaa ccacaaccca gagtcaggga aggtgcgagt ttgaagttat
     1741 acaggtcacc gataccaccc tgagacgccg gggtgttgat cagaatacgg gccgttttca
     1801 ttttctgacc gaagtaggca acgcgagccg gttggttatc ctggtcagtg tacaggcaag
     1861 aggtatgacc gatgccgccc atagcaacca gtttctctgc tttttctacc gcgtcttcga
     1921 aatctttagc acggtacatt gccagagtcg gggacagttt ttcatgtgcg aacggttcgc
     1981 tttcatcaac aacggtcact tcaccgatca gaatcttggt attttctggt acagagaagc
     2041 ctgccagttc agcaatttta taggctggct gaccaacgat agccgcgttc agcgcaccgt
     2101 ttttcaggat aacatcctga acagctttca gctctttacc ctgcaacaga tagccgccgt
     2161 gggttgcgaa acgttcacgt actgcgtcat aaacagagtc aacaacaaca acagactgtt
     2221 cagaagcaca gattacgccg ttgtcgaagg ttttggacat cagcacagat gcaactgcac
     2281 gtttgatatc agcagtttca tcgataacaa ctggcgtgtt acccgcgcct acaccaatag
     2341 ctggtttacc ggagctgtat gcggctttaa ccatgcccgg accaccagtc gcgaggatca
     2401 ggttgatgtc tgggtggtgc atcagtgcgt tagacagttc aacagaaggt tgatcgatcc
     2461 agccgatcag atctttcgga gcaccggcag cgatagcagc ctgcagaacg atatcagccg
     2521 ctttgttggt tgcatctttt gcacgcgggt gcggggagaa gataatggcg ttacgggtct
     2581 tcaggctgat cagcgatttg aagatagcag ttgaagtcgg gttagtggtc ggaacgatac
     2641 cgcaaataat accgattggt tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca
     2701 gaacaccaca ggttttttca tctttatagg cgttatagat atattcagaa gcaaagtggt
     2761 ttttgatcac tttatcttcg acgataccca tgccggattc ggcaacggcc attttcgcga
     2821 gtgggattcg agcatctgca gcagccagag cggcggcgcg gaagattttg tctacttgct
     2881 cttgagtgaa actggcatat tcacgctggg ctttttttac acgctctacg agtgcgttaa
     2941 gttcagcgac attagtaaca gccataatgc tctcctgata atgttaaact tttttagtaa
     3001 atcatctgct cgaatacgag agtatagtca gtgcggtgat gatttgctta accgatgaaa
     3061 atcaaaagct tactcgcgct cacactcact gtgatttact aaaagagttt aaacattaga
     3121 gttattatct ctaatgcgtc acttccaggt ggcgtaagca agattactca cttctgggta
     3181 ctgattacgt gatccaaatc aaatttttgc aaagctgaca cctttcagca tcgcttttcg
     3241 ccattatagc taacagttaa taaattgtag tatgatttgt tggctacatt agcatgtttt
     3301 gcacaaccag ataacaataa cgaatgatag caattttaag tagttaggag gtgaaaaatg
     3361 ctgtcaaaag gcgtattgtc agcgcgtctt tttaacctta tttatggcta acattatccg
     3421 gcttttgctt cggagctaac cgtgattcag accctttttg attttcccgt ttacttcaaa
     3481 tttttcatcg ggttatttgc gctggtcaac ccggtaggga ttattcccgt ctttatcagc
     3541 atgaccagtt atcagacagc ggcagcgcga aacaaaacga accttacagc caacctgtct
     3601 gtggccatta tcttgtggat ctcgcttttt ctcggcgaca cgattctaca actttttggt
     3661 atatcaattg attcattccg tatcgccggt ggcatcctgg tggtgacgat agcgatgtcg
     3721 atgatcagcg gcaagcttgg cgaggataaa cagaacaagc aagaaaaatc agaaaccgcg
     3781 gtgcgtgaaa gcattggtgt ggtgccattg gcgttgccgt tgatggcggg gccaggggcg
     3841 atcagttcta ccatcgtctg ggggacgcgt tatcacagca ttagctatct gtttggtttc
     3901 tttgtggcta ttgcattgtt cgctttatgt tgctggggat tgttccgcat ggcaccgtgg
     3961 ctggtacggg ttttacgcca gaccggcatc aacgtgatta cgcgtattat ggggctattg
     4021 ctgatggcat tggggattga atttatcgtt actggtatta aggggatttt ccccggcctg
     4081 cttaattaat tcctttcaaa tgaaacggag ctgtcatgct ccgtttactt cgtcattatt
     4141 tttactttgt tcccgcgcag ttatcaaaag caaaaggaat gggtgaaaat attcttctca
     4201 aattacagtt agttataagg atttccttaa ctgcttctcc tcaccatcat gttattttcg
     4261 ccacatcata atcctgggct tgctgaagaa taattgaaat gatattagta attccactgc
     4321 ctttggtaga ggaaagtgct aaataataat caattgttaa attattgtgc atttcactac
     4381 tggaactgta atcagaaaag agagacatgc ttaaccaatc tctatttgat tgaattgaaa
     4441 gatgtttgtt aagacatgga tgcaagctat agattctgat gcggtcaata aaagagaatt
     4501 gcttaacaat tttgcaaaat gtattggcga gtaagaagcg catttggtac tttccgggca
     4561 accgccaggc gattctttat tggtaatgag aataattaac aattaaagag cgtcgcgaaa
     4621 gaataatgtg tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat
     4681 caccagaata aataaagtcg gtgatagcaa tccgtaacga taaagtaacc tgacagcaga
     4741 aagtctccga gcctgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta
     4801 attataatga gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag
     4861 ctggcgttct ggctgcacta atggcaggga atgtcgcgct ggcagctgat gtacccgcag
     4921 gcgtcacact ggcggaaaaa caaacgctgg tacgtaacaa tggttcagaa gttcagtcat
     4981 tagatccgca taaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag
     5041 gcttactggt cagcgatctt gacggtcatc cggcacctgg cgtcgctgaa tcctgggata
     5101 ataaagacgc gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca
     5161 cgccagtcac agcacaagac tttgtgtata gctggcaacg ttctgttgat ccaaacaccg
     5221 cttctccgta tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg
     5281 aagggaaaaa accgattacc gatctcggcg tgaaagcaat tgatgatcac acattagaag
     5341 tcaccttaag tgaacctgtt ccgtacttct ataaattact tgttcaccca tcaacttcac
     5401 cggtgccaaa agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatca
     5461 tcaccaacgg tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac
     5521 gcagcccaac ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta
     5581 ttgcttctga agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacgtata
     5641 acaacatgcc gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg
     5701 ttgatccata cctgtgcact tattattacg aaattaacaa ccagaaaccg ccattcaacg
     5761 atgtacgtgt gcgtaccgca ctgaaactgg gtatggaccg cgatatcatt gttaataaag
     5821 tgaaagcgca gggcgacatg cccgcctatg gttacactcc accgtatact gatggcgcaa
     5881 aattgactca gccggaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa
     5941 aactgctggc tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgttgtata
     6001 acacctccga tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca
     6061 ttggtgtaaa cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc
     6121 agggcacttt tgatgtggcc cgtgcaggct ggtgtgcaga ctacaacgaa ccaacttcct
     6181 tcctgaacac catgctgtcg aacagctcaa tgaataccgc gcattataag agcccggcct
     6241 ttgacagcat tatggcggaa acgctgaaag cgactgacga ggcgcagcgc acagctctgt
     6301 acactaaagc agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg
     6361 tgaatgcgcg tctggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata
     6421 atacctacac ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggcgt
     6481 gtactgctcc acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg
     6541 gcaatgttaa aatttattct acgtcgctgt ctggaagcga ttccgacgct atttattctt
     6601 attactattt cgttcttcat gatgcgcctc gcgccgggaa gcccttttac cggcgaacgt
     6661 actttaccgc cagaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc
     6721 atgacacagt atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg tccatcgttt
     6781 aaatataaag attattcggt caacgacctg gttgcatcca gttttcccgt ttctgccaaa
     6841 ctgggagccg cagcattttt ccttgcggta atactgggtg ttagtgctgg cgttattgcc
     6901 gcattaaaac aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt
     6961 gttatcccca gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat
     7021 tggctgccgg gcggtggctg gaatggtggt gcgcttaaat tcatgatact gccaatggtg
     7081 gcgttgtcac tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgaa
     7141 gtattacact ccaactttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc
     7201 attttacgcc acgcattaaa acctgctctg ttacccgtgc tctcctatat gggccccgca
     7261 tttgtcggca ttattaccgg ttctatggtc atcgaaacca tttatggttt gccggggatt
     7321 gggcaactgt tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagcctgact
     7381 atcctggttg gtgcgttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt
     7441 atcgacccga aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca
     7501 gcgagacgct ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg
     7561 acgcacgtcg acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc
     7621 taatcgcgtt atttgtaatc ctggcaccga tgctttcgca gtttgcctat gacgatactg
     7681 actgggcgat gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact
     7741 catccggtcg cgacctgctt gtgcgtgttg cgattggcgg gcgtatctca ctcatggtcg
     7801 gtgttgctgc ggcactggtg gcagtggtcg tggggacact ttatggttcg ctttccggtt
     7861 atctgggcgg taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc
     7921 cattcatgtt cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc ctgctgattt
     7981 tcgtggcgat tggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc
     8041 tgagtctgaa gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacgtcgg
     8101 gcattgttat tcgccacatt gtgccgaatg tactcggtgt ggtggtggtc tacgcatcac
     8161 ttctggtgcc cagcatgatc ctctttgaat ctttccttag cttcctgggg ttggggacgc
     8221 aagagccgtt aagcagctgg ggcgcattgc tgagtgatgg cgcgaactcg atggaagtct
     8281 ctccatggtt actgttgttc ccagcgggat tcctcgtggt gacgctattt tgtttcaact
     8341 ttataggcga tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat
     8401 gagcgtaatt gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa
     8461 cgtgaaagat ttgcgtgtca cctttagtac cccggacggc gacgtcacgg cggtcaatga
     8521 tttgaatttt tccctacgtg ccggagaaac gctgggcatt gtaggtgagt ctggttcggg
     8581 taaatcgcaa actgcatttg cgttgatggg cctgctggct gccaacggac gtattggcgg
     8641 atcggcaacc ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac tcaatagact
     8701 gcgcgctgaa caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat
     8761 gcgcgtcggt gagcagttga tggaagtgct gatgctgcat aagaacatga gcaaagctga
     8821 ggcgtttgaa gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag cacgtaaacg
     8881 catgaaaatg tacccgcatg aattttctgg tggcatgcgt cagcgagtca tgattgcgat
     8941 ggcattgcta tgtcgaccta agctgctgat tgcggatgaa cccactactg cgctggacgt
     9001 caccgtacag gcgcagatca tgacgctatt aaatgaactg aagcgggaat ttaataccgc
     9061 catcattatg atcacccacg atcttggcgt ggtggcgggg atctgcgaca aagtgctggt
     9121 aatgtacgcc gggcgcacga tggaatatgg caacgcgcgc gatgtctttt atcaacccgt
     9181 tcatccttat tctatcggtt tgctcaacgc ggtgccgcgt ctcgatgcgg aaggtgaaac
     9241 aatgttgacc atccctggta atccgccaaa cctgctgcga ttaccgaaag gttgcccgtt
     9301 ccagccacgt tgtccgcatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt
     9361 tacgcctggc cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta
     9421 accgaaggaa gaaaagtcct cctcgaaatc gccgatctga aagtgcactt tgaaatcaaa
     9481 gatggcaaac agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtcact
     9541 cttcgcctgt atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc
     9601 acctttgctc gcgccatcat tggtttggtc aaggcgaccg acggtcatgt tgcctggtta
     9661 ggtaaagagt tgctgggcat gaagcccgat gaatggcgtg ccgttcgcag tgatattcag
     9721 atgattttcc aggatccatt ggcatcgctt aacccgcgta tgaccatcgg cgagatcatc
     9781 gctgaaccac tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg
     9841 aaggcgatga tgctgaaagt cgggttattg cctaacctga ttaaccgcta tccgcatgag
     9901 ttctctggtg ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag
     9961 ctgattatct gtgatgagcc ggtgtcggcg ctggacgtgt caattcaggc gcaggtggtc
    10021 aacctgctcc agcagctgca acgcgagatg ggattgtcat taatttttat cgctcatgac
    10081 ctggccgtgg taaaacacat ttccgatcgt gtgctggtga tgtatctcgg ccatgcggta
    10141 gaactgggga cctatgatga ggtctatcac aatccgctac atccttacac cagggcattg
    10201 atgtcggcag tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg
    10261 gaaggggaat taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgc
    10321 ccgattgccg gtccagagtg tgccaaaaca cgtcctgtgc tggaggggag tttcagacac
    10381 gccgtttctt gcctgaaagt cgatccgctt taaaagcaat aagggctgac agttgtcagc
    10441 ccttattcac gccaaaagcg attatttatt cccgccagat gatatggcac agtttgtgat
    10501 ctttttcgcg gcataacaga atgcgcgcga aaacatcgtt gatttcacca tcttcactgt
    10561 ccgccagacc aatcaccact tcggcaaaaa agtccgggtt caaatcgaaa tcaacatgtt
    10621 cctgccagtc ttctgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat
    10681 tgaacagcag aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt
    10741 aggcctgctc aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca
    10801 ctacctgttt aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa gtaaaacagt
    10861 cgctcggcga cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacgt
    10921 gaacgcgaaa tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc tttatcgtcg
    10981 attgccaggg taatctcgaa atttagccac agactccgca tatcaaggtt aacggtgcca
    11041 accagactta gttcaccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac
    11101 tgataaattt taaccccagc agccagcagt tccgtaaaga atgcgcgact ggcccagccg
    11161 accagcatcg agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc
    11221 gccgtgcaaa tcgcatgaag taaatcatcg ctgggcacaa agtagggcgt tgtcatgatc
    11281 aaatattcac gcgccgaata agcagcagtc aataatgcct ggtgaatgag atcttccgga
    11341 aagccggggc cagaagcaat tgtgtgaatg gtgtgaccgc tggcctgctc aaacggcata
    11401 atattgacat ctggtggtgg cggcagaata cgttttccgg tttcaatctc ccagtcgcag
    11461 gaataaataa tccccatcgc ggtagcgatg ggaccttcca tacgcgccat cagatcaatc
    11521 cattgcccta cgcccgcatc ttgtttgaag tagcgagggt cgaccatatt catgctgccg
    11581 gtgtacgcga tgtaattatc gatcatgatc atcttgcgat gttggcgcag gtccatacgg
    11641 cgtaaaaaca cacgcatcag attgaccttt aaggcttcga caacttcaat accggcatta
    11701 cgcattagct cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc
    11761 aatcggcaat gaatgccgcg tcgcgcagcc gccattaatg attcagccac ctggtccgcc
    11821 atgccgccgg gctgccagat ataaaacacc atctcaatat tatggcgagc gagctggatg
    11881 tcgcggatta acgcctgcat cacatcatct gactcggtca tcagttgcag ctgattccct
    11941 ttgaccccag cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg
    12001 ctattttctt cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg
    12061 gaaggccaca tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg
    12121 gcaagatagg caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc
    12181 gcggagggaa ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac
    12241 cagtatccca gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa
    12301 tccttttgaa agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac
    12361 gcgggaaaag cactggtcag cgtcgggggc gggggtataa tgaccattct gttattgcat
    12421 agagtagtta acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag
    12481 gctagccgac gtaaatcgcg ttggcttgag gggcagtcgc gccgaaatat gcgtatccac
    12541 agcatcagga agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat
    12601 atctaaatgt actgggcacc attgcggtgc ccaataagtt tactcttccc tctttaaact
    12661 ctatttttgc gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa
    12721 aatgccatga ccggagttca acaacattga aacgagcata tcattattaa tctcttcacc
    12781 atttaaaact cgcgccagaa tttcgctgcc aaacagttgc ggcgagagaa tgatatccgg
    12841 atgtaccatc ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac
    12901 atcactgctc atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact
    12961 cagcgccaga atggcccggc atcgatcaat tccagctttc tttaatactg aactgtcatt
    13021 actgtcaccg gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc
    13081 atcttcaggc aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat
    13141 cgtattgatg gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt
    13201 atgattgttt ccttttacaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga
    13261 ggtcatagat gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg
    13321 tgctgattca gaaacaggga caatatcgcc atagccgacg gttgacatgg tttctatcga
    13381 aaaatagaac gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag
    13441 cgcaccgtag gtggagtaaa acagtaacgt cgtgaaacta ataaatgcga aaattgtccc
    13501 ggctgcggcg ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa
    13561 aatgcaaaat ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc
    13621 tatcaacaac aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat
    13681 ggagttcagg acaagaaaga cgccgagcat aaagagtggg gcgtttgcca gcaaggagag
    13741 atccaactct gaaaatgctt tgatatggaa aatatctagc aaattgaccg acataccata
    13801 gattgtttta aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg
    13861 tagggtcacc cataaattag ttgctgtttg tttgaatgta gcccagtgac tcactgatac
    13921 ctctgtaacc acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca
    13981 gcgcgtgaat gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag
    14041 tgagcaactc tgcgtcagga aagggcattt ttcctgaata tgctcacatc atataaagaa
    14101 atacagataa agttattatc tgcttgtggt ggtaaatgca atgactgacc tataaggaaa
    14161 ggccaaacaa gaacacggtt gctaaaaccg tgcccttaaa tattgaattt ctattcagaa
    14221 cactttctta aatggtttca ctgaaacgtg ttcatagact cctgccgcta cgtacgggtc
    14281 agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa cggttgagcc
    14341 agtaaatccc gcagcccctg gatcgttact gtctactgct ggcattggac cggcggtcaa
    14401 caatcgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac gaacggaaag
    14461 gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca cgggcacaac
    14521 tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct gcaacggaaa
    14581 gatgatgtct ttgttaagac catttaaaaa gtaaggtcaa ttacgccaaa aatgacattt
    14641 tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt taaaatcgag
    14701 gcctggtttt tctactgaaa tgattatgac ttcaatgacc cttgatttac ctcgccgctt
    14761 cccctggccg acgttacttt cggtctgcat tcatggtgct gttgtggcgg gtctgctcta
    14821 tacctcggta catcaggtta ttgaactacc tgcgcctgcg cagccaattt ctgtcacgat
    14881 ggttgcgcct gcggatctcg aaccgccaca agccgttcag ccgccaccgg agccggtggt
    14941 agagccagaa ccggaacctg agccgatccc cgaaccgcca aaagaagcac cggtggtcat
    15001 tgaaaagccg aagccgaaac ctaagccaaa accgaagccg gtgaaaaagg tacaggagca
    15061 gccaaaacgc gatgtcaaac ccgtagagtc gcgtccggca tcaccgtttg aaaatactgc
    15121 acccgcgcgc cctacatcaa gtacggcaac ggctgcaacc agcaagccgg ttaccagtgt
    15181 ggcttcagga ccacgcgcat taagccgcaa tcagccgcag tatccggcac gagcacaggc
    15241 attgcgcatt gaagggcagg ttaaagttaa atttgatgtc acgccggatg gtcgcgtgga
    15301 taacgtacaa atcctctcag ccaagcctgc gaacatgttt gagcgtgagg tgaaaaatgc
    15361 gatgcgcaga tggcgttatg agccgggtaa gccaggcagt gggattgtgg tgaacatcct
    15421 gtttaaaatt aacggcacca ccgaaattca gtaagcagaa agtcaaaagc ctccgaccgg
    15481 aggcttttga ttattactca acaggtaagg cgcgaggttt tccttcagga tcaaccgcga
    15541 catacttaaa taatgcttct gtcgctttat agcgttgccc gatgggttca gacgcgactt
    15601 ttttcaccca cacttcaata ttaatgctaa ccgatgtcgt ccctttctgg acacagcgtg
    15661 catagcagca caccacatcg ccgaccgcaa ccggtcgtaa gaaagtcatt ccttcaaccc
    15721 gcacggtcac tacgcgaccg tgggcaattt ctttcgccag aatagcgccg ccaatatcca
    15781 tttgtgacat taaccaacca ccaaagatgt caccattggc attggtatcg gcgggcatgg
    15841 ctaaagtacg taaaacaaga tcgccctgag ggacgttatg tgttgtagac atggtaaaac
    15901 cgacttaaaa ggaaaatcac aggcgcgatg ctactatgat tttcctgagg agaacagagg
    15961 agatacagcg ccagccccga aggactggca gtctggttag gatttatctt cctgcggcat
    16021 gtggcggtag atatagatac cgcttaacaa tgtgaagatt aaggtaaggg cggtcaggcc
    16081 aaagacttta aagttgaccc aaatattttg cggcagccag aatgcgatgt agatgtttgc
    16141 cagaccgcaa aggataaaga aaacagccca ggccagattc agcttcgacc ataccggttg
    16201 cggcagcgtg agttctttac ccagcatccg ctgaattagc ggctttttca tcacccattg
    16261 gctgactaac agggcacccg caaacagggc ataaatgaca gtaaccttcc atttaataaa
    16321 ctcatcattg tggaagaaca gggtcaagcc accgaagacg accaccagaa caaaagtgat
    16381 cagggccatc ttctcaacct tacgaaagcg aacccagcta tatataagca caatcgccgt
    16441 ggcgacgatc agcgccgcag tagccgcata gatgtcataa atcttgtaaa acgcgaaaaa
    16501 gacaaccagc ggtaaaaaat caagaaactg cttcatttta cgattccgta atcaaatgct
    16561 taaggttatt ggcgaatcaa catatacagg cggaacagat agatgagcag tacggctgaa
    16621 atcaggttgc tcaaggtgtt cgccagtacg gcaccaattt ccggggttaa tgcggcaaaa
    16681 gaagaggcaa aaagcagcaa cagtgtcttt gccagcaacc agctcagtac tgcgggtgcc
    16741 accagacgca tattcgccca agtcagccgc atactgctac gcatcgaggc aaaaacgccc
    16801 attttgtcct gtaccaacat caccggtgcc agggccagta aaatggccat gataattccc
    16861 ggaacgacca ccagcatgat gccaatctgt actaaaaggg tagtcagaaa aatcagaata
    16921 aataactttg gcaatatcgg cgcactggca ccaatagctc gcagcgcact gactctctga
    16981 cccgcagaca ccagctggat aattaatatt acgcctccgg cgagaatggc gttaccgatt
    17041 aatcctgaaa aagtggacgc cgctgaagcc tgcagcaaaa tttgttgctg ttccggtgac
    17101 atattctgaa ccaggtcgaa caacccacta ctgccgctaa cgggcacgcc gtcattgagc
    17161 tgcgcaagct gtgcatcact gggtgagaaa acatgcccta acaccactgt gataaacgca
    17221 catagcaacg ataccaacag aatggtcata aattgattac ggaagaaatt tccggtgtca
    17281 cggtatacgg actgcgccgt gatagacatg cactctcctt gagttttatg caggtgttaa
    17341 ttagcgggta attgtaccct ggttaagcct cgggtggcag catcaggtct tgtatggaaa
    17401 agcatatctt tgtaaagcgg aggtaattgc tggccttcag acgtgggcaa atcgcggcaa
    17461 gtggcgatcg agttaatatt tgcgtagcga aaatatttaa aaattgattt aaatcacatt
    17521 aaccaggatt ctcaatgcaa cttctaaatt aatccagatc aataaatggt gaattatcat
    17581 atgtaatgtg atctatgtag gatcatttgt tactccaatg taggtatatt cgtcacgttt
    17641 ttataaccat aacgacggag cggatatgaa aaagttaaca gtggcggctt tggcagtaac
    17701 aactcttctc tctggcagtg cctttgcgca tgaagcaggc gaatttttta tgcgtgcagg
    17761 ttctgcaacc gtacgtccaa cagaaggtgc tggtggtacg ttaggaagtc tgggtggatt
    17821 cagcgtgacc aataatacgc aactgggcct gacgtttact tatatggcga ccgataacat
    17881 tggtgtggaa ttgctggcag cgacgccgtt ccgccataaa atcggcaccc gggcgaccgg
    17941 cgatattgca accgttcatc atctgccgcc aacactgatg gcgcagtggt attttggtga
    18001 tgccagcagc aaattccgtc cttacgttgg ggcaggtatt aactacacca ccttctttga
    18061 taatggattt aacgatcatg gcaaagaggc ggggctttcc gatctcagtc tgaaagattc
    18121 ctggggagct gccgggcagg tgggggttga ttatctgatt aaccgtgact ggttggttaa
    18181 catgtcagtc tggtacatgg atattgatac cactgccaat tataaactgg gcggtgcaca
    18241 gcaacacgat agcgtacgcc tcgatccgtg ggtgtttatg ttctcagcag gatatcgttt
    18301 ttaattccgc acaaaaatga ccccgtaatt acggggtcaa taaggacatg gtataaaggg
    18361 tattatttct tcgcttctac gccatcagtt tcagagcgaa ttaagaattt ctcagttgtt
    18421 tgcggaatat gctgaattag ccagtcggcc atgtgctttt catcatttaa aatcgcttcg
    18481 ataatcggaa ttgaggctgt atcaccggca ttttttgctg ctgctaagag tgaggtataa
    18541 caggcgattt caaattgctc gaagacatat ccgctaatag agcctttgac tatttcatca
    18601 gaagggaata taccaccgat tgactgccca agtgcagcca ttttactcat ggaatcttta
    18661 atgactgaac gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt
    18721 ttggtttcac taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacga
    18781 ctggccatgg attcaagcat tgattcggct tgcttttcca ttgcgtgggc gtcacgtaac
    18841 cagtcatgat aatgttcaat acgattcatg aaaatgtcct ctttataaaa tgactaaaag
    18901 ttaaagttca tatttcaggc tttattttcg gctttcttat ttacgttatt aagggccaga
    18961 tcagtcaatt tgatgtcggt ggccttttct tcttccaggg tctctttcag aagcttcgct
    19021 gctttacgat aacctaattg ttcagccagc gtcgctaatg tcccgtaact ggcaatctca
    19081 taatgctcga ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc gtttttctcg
    19141 gtactttcga tgacctcatt agcttcttca ataagacctt ccattgccac acatttcatg
    19201 cgcttaattt tcagattcga ttctgattcc acaacttgat caatacgttc aatctgtcca
    19261 tatgtttcct cgaggtgcgc atgaaaagcc tgacttaatt tttcgtttga tgttgctctt
    19321 gcgagttttg ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg
    19381 tgaataaata catcttcaat ggtcttcata ttcatatttt tctccagtga aatcactgcg
    19441 agccatacgg cccgcagatg caattatctt tatcagctga tattaataat taacggtgaa
    19501 tcaggatttg cctgatttat taccaccgct ttgttgaccg ccttttttac ccgcttcaga
    19561 tgcgcgttgc ggatcatttt taaaattacc gccgctatgc tgaccgcctt tacggccagc
    19621 gtcggatgcc ttttcacggt cttcggcgaa atttcctgaa ccaccacgat gttcggccat
    19681 gttatttctc ccgttgcgtt gcattgtttc attaatatgg gtgttgtgtg tcgacactca
    19741 ttaaaattag tcgctaatga aaattagtca aattaagcgc aacgagaaaa aagagggaaa
    19801 atatattatg aggaacattc tggatatatt aacaattgcc tgaggaataa gtgacttaga
    19861 gaaaattatt gattttactg gtgttatgtt gcgggattaa tttgtttgat tatattactg
    19921 ttgggcggaa aaaagacgta agttgacgtt cgacaggggc aagcgaaacg gtaaaaagat
    19981 aaataataaa tgaatttagg atttttccag cttcgttaaa gaaagttaaa atgccgccag
    20041 cggaactggc ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg tacaaaagct
    20101 ttcagtgccg ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacgatg
    20161 gccgaaccag aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac ctgatccggg
    20221 gcggaaatac caaagccctg caatggaggc gcagcattgt attctttcag cttcgtgacc
    20281 agatgattga ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc acgtgacaat
    20341 aaataggtgt atccacgacc gtaggaggct atctggcgca gcaaatcatc gtcggcattc
    20401 ggcgggcaga taaagatagg tgcaagatta tgacgcaacg cggcctggcg gaagggagcg
    20461 gactcttcca ctggcacatc ggcaaccagc accgaatcga cgccgacttt ctcgcactgg
    20521 gcataaaact catcaatgcc tttgttaaac accaggttgg catacatcag caggccaatg
    20581 ggaatggtcg ggtgtttctg gcgaatcagt gccagcattt caaaacattg tgccggagtc
    20641 acacctgccg caaaggcgcg cagagtggcg ttttgaatcg tcgggccatc cgccagtggg
    20701 tcggagaagg ggatacctaa ctccagcgcg tcagcaccgg cttcaattag cgtgtcgata
    20761 attttcagcg actgctcaat gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct
    20821 tctttgcgct ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc
    20881 gtgctttcaa aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa
    20941 ccaccagtag ctgctctttt tccgggtttt cgcgcatcat tttcagcgca tgggccaggg
    21001 cgtgtgagga ctccagcgcc gggataatcc cttcgtgcag gcagagcgtt ttgaaggctt
    21061 ccagtgcttc atcatcggta attgacacgt aatcagcgcg tccagtgctg ttaagatacg
    21121 cgtgttgtgg gccgacagac gggaaatcca gtccggcgga gatggagtaa gattcttcaa
    21181 tctgcccgtc ttcggtttgc atcatcggcg ctttcatacc gaaatagatg cccacgcgac
    21241 cgtgttttaa cggtgcgccg tgctcgccag tttcgatacc gtgaccacct ggctccacac
    21301 caatcaggcc gacgttggtt tcattgatga aatcagcaaa catgccgatg gcattcgaac
    21361 cgccgccaac acaggcgata acagcatccg gcaggcgacc ttctctttcc agaatctgcg
    21421 ctttcgtttc ttcgccaatc atccgctgaa actcacgcac aatggtcggg taaggatgcg
    21481 ggccagccgc ggtgcccagc atatagtgcg cggtttcata actgccggac cagtcgcgta
    21541 gcgcctcatt acaggcatct ttcagcgtcg cggaaccgct atgcaccggg atcacttccg
    21601 cacccattaa acgcatacga aaaacgttag gcgactggcg ttcaacgtct ttggcaccca
    21661 tataaatgcg acatttcagg ccgagcaggg cgctggcaag ggccgacgcc acgccatgct
    21721 gaccggctcc ggtttcggcg atgatttccg ttttacccat ccgcttcgcc agcaacgcct
    21781 ggccgagcac ttggttggtt ttatgcgcgc cgccgtgcag caaatcttcg cgcttgagat
    21841 acagcgtggt gttagtgcca gccgtaatgt tctggcattt ggtgagtgcg gttggacgcc
    21901 cggcatagtt tttcagcagg tcgttgaact gagcctgaaa ttcaggatct ttttgcgcgc
    21961 taacaaaagc ttcttccagc tggcgcagag caggcatcag gatttgtggc acgtacatgc
    22021 cgccaaactc accaaaatac gggttaagta atgttgtcat tgttcctttc cttaatatgc
    22081 gcgcagcgtc tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc
    22141 tacagcagaa ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca cgcagttatc
    22201 tgcgcctaag ccccccgcca gcagaacgtt gccaagcgat tgaccgttta acagtgacca
    22261 gtcgaaacgt tgaccgcttc caccctggcc gttgtctaaa acatatttat caacgtgctg
    22321 aagctcgcgg gcgggcaggg tttcaccgac gcttaatgct ttccagatgg caacgtgtgc
    22381 tggcagagct tcacgcagcg tatcgatata cagctgatct tcattaccat gcagttgcac
    22441 tgccgccagc gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa
    22501 cacgccaaca tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac
    22561 gcaacgcggt gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc
    22621 tgctttagca tcttgcccac gcgtcaggcc acatacttta ttctcaccca gcaatacccg
    22681 gcgcacggcg gcgttcaaat cgtcatgggc catcaacgcc gaaccaatca gaaaaccgtt
    22741 agcgaagtgg cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac
    22801 cgtcacgttg tgccccagtt tcggcgcaag ctcgcgggta cggttgagat caatcgacaa
    22861 atcacgcaga tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa tggcgcgctc
    22921 cagttcctct tcattactga cttcggtcag cacacccatc tccagactgt gggcgacggc
    22981 ggcaaggtgg cgatattgtt cgtcatccag tactgaaagc attaataagc aggcatcggc
    23041 ctggtaatag cgcgccagat agatctggta agggtcgata ataaagtctt tacataaaat
    23101 cggctgcggg gcgatttggc tgacgatgga gaggaaatca aagctcccct gaaaatattt
    23161 ctcatcagtc agcaccgaaa ttgccgaagc gtaatttctg tagacggttg caatgtgtgc
    23221 cggatcaaaa tcatcgcgga tcacgccttt tgacggtgac gcttttttac actccagaat
    23281 aaacgccgtg cgtgcaccct gtagcgcatc ataaaaatgt cgcgtgctcg gctgaacctc
    23341 attctgaaaa ctggccagcg gttgctgctg tttgcgggct tctacccaaa tcgccttgtc
    23401 tgcgacgatt ttcgctaaaa cggtttgcat catttaccct cgtgccgcca gtgcggtgac
    23461 tctgtcgtaa gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag
    23521 atcttcatgg ccatgcaggc gcattaacat ggcgacgttc gccgcgacgg ctgcttcatg
    23581 ggcggcgtcg cctttacctt gtaacaagcg tgttaaaatg tcacggtttt cttccggtgt
    23641 tccgcctgcc agttgctcct ggtggtaggg tgtcaggcca aaatcttctg cagttagctg
    23701 atagctctta atttcaccgt catgcagttc ggcaacgatt gtcggcgcgt gtaatgaaac
    23761 ttcatccatc ccgccgctgt gcactaccgc agcgcgttga taccccagca cgcgcaaggt
    23821 ttcggcaatc ggcagcacca gttccggact ataaacacca attagcgcca gcggcggatg
    23881 cgccgggtta atcaatggcc ccaacacgtt gaacagggtg cgggttttca gttgctggcg
    23941 aaccggcatc gcgtggcgga atccggtgtg atatttcggc gcaaagagga aacagacgcc
    24001 taactcatcc agcgcctggc gcgatttatc ggcgttcata tcaagattaa taccgaacgc
    24061 cgccagcaga tcggacgagc cggatttact ggagacgctg cggttaccgt gtttcgccac
    24121 tttcagccca caggccgcgg cgacaaacgc actggcggta gaaatattga tgctgttgct
    24181 gccgtcaccg ccagtaccga cgatatcggc aaacagataa tccgggcgcg ggaacggcgc
    24241 ggcgttttcc agtagcgcgg ttgctgctcc ggcgatctcg ttcgggtgct cgccgcgaat
    24301 tttcatgctc accagcgccg ccgccagttg ttccggcttc agctcgccac gcaccaccgc
    24361 tgaaaacagc tggtgacttt cttgttggct aagcgtctgt gcctgataca gtttttccag
    24421 aatcggttgc agcgtgttgg ttggctcaag tttctgctgc gcccaggcca gcgtttgttc
    24481 cagcaggcga gcgccctggg tagtaagaat ggattccgga tggaactgga aaccgcaaac
    24541 gcgatctgca tcgtgacgca ccgccatcac catgccatta aaatgggcgt tgatggttaa
    24601 acctgccggg atattactgc caaccagcga gtgataacgc gccaccggca atgggtttgt
    24661 taatccggca aacatcgcct gactgtcatg ttcaatgctc gacgctttac cgtgaagaat
    24721 ttcgcccgcc tgaccgacat agcccccgta agcttcgaca attgcctgat gtccgaggca
    24781 aatgccaata attggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc
    24841 ttcgctcggc acaccggggc caggagaaag catcagcacc ggattgctca tcgtcgccag
    24901 gcgttcaatt aaggtctgcg ccggaacatg gttgcggtaa atcaccacgt tatgaccatt
    24961 gctgcgcaac tgatctgcca ggttgtacgt aaaagagtcg atattatcga gcagcagaat
    25021 gtcagccatc agaacgtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg
    25081 cgggctttat tacgggtttc gtcggcttcc gactgcggaa cagaatcaag gactacgcca
    25141 gcaccggctt gcacggtggc gataccgttt tccaccagcg ccgagcggat cacaatgcag
    25201 gtgtcgagat cgccatgcgc ggtaaaataa cctaccgcgc cgccgtagct gccgcgtcga
    25261 cgaccttctg cttcggcaat taactgcata gcgcgcactt tcggcgcacc gcttaatgtc
    25321 cccatattca tacaggcgcg gtaagcgtgc agggcgtcga gatcgtggcg cagctcaccg
    25381 accacgcggg agacgaggtg catcacataa gaataacggt caactttggt gagatcggcg
    25441 acgtagcgac taccgggagt gcaaatacgc gccaggtcat tacgggcgag atccactagc
    25501 atcagatgtt cagaaagctc tttatgatcg gtacgcattt ccagttcgat gcggctgtcg
    25561 aggtctctgt ccagtgaacc atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg
    25621 tagatctcaa tctggcggct ggtggcgtca tacttgagcg aactttccgg cgacgcgcca
    25681 aacagggtga aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc
    25741 agcacgtaat aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc
    25801 acctggaaaa tttctccggc gcgaatcgct ttttgcagcg aacgcactac gccaccgaac
    25861 tcttcatcac tctggttaca ttcacaacgc atatgcggca cggaaaccac cggcagcggc
    25921 ggcgcggctt cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt
    25981 tcttcttcat tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca
    26041 atcaccatca gcgtttcagc gagataaaaa cagaaatcag gacagttatt ttccgctgcc
    26101 agttgtggta aatcttcaaa tcccgccaca aggtcataag agaacagacc gccgaagaac
    26161 attgcttctc gttcttcatt cggtacattc aacagactct gcaataagcg gaaagcgtca
    26221 aaaaccgaaa gggagcataa gcgagcgtct tcatccagca gtggactgac aggggggaag
    26281 cgcagcacac ggcagtttgg tgattgttca ttttccacac ccgcaggcag ggcgttatcc
    26341 agtagtgcca gcagggcttc gccattgccg gaaagtgcct ggattgtgac agtgtcacct
    26401 aaagctgtaa tgcgcagcgc actgtctacc agcagcaggc tttttaaatc atctttgctg
    26461 tcgatatctg cggattccag tagcagcgtt gctggacgat cctcacacaa ctggtgaaaa
    26521 agcgcggtgg gattgtcgcg ataagcgcct tcgcaggtta gcagttcgag agtcggtttt
    26581 tgtgtttgca ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct
    26641 gggtatctga ttgctttacg catggtgaat acactgcccg tttcaggaag tgcgccacca
    26701 acctttcagt acgaaaattg ctttcattgt cgataccctt tttacgtgaa cttgcgtact
    26761 agttaactag ttcgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt
    26821 tatgatgtcg gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt
    26881 acgcagtgat ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag
    26941 aagcattggt gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc accgaccatg
    27001 acaccacagc tgcaatctca ccagcaagag aggaaatttc acgttcggga ctggcgctga
    27061 atcttattcc cggcgtggaa atttccacgg tctgggaaaa tcatgaaatt catattgtcg
    27121 ggctgaatat tgatattact catccgctaa tgtgtgagtt cctcgcgcag cagacagaac
    27181 ggcgcaatca gcgggcgcag ctgattgccg aaagacttga aaaagcgcaa atacctgggg
    27241 cactggaagg cgcgcaacga ctggcgcagg gcggtgcggt gacgcgcggt cattttgctc
    27301 gtttccttgt ggagtgcggc aaagccagtt caatggcgga tgtctttaaa aagtatctgg
    27361 cgcgcgggaa aaccggatac gttccgccac agtggtgtac aatagaacaa gctattgatg
    27421 tcattcatca ttctggcggt aaggcggtgc tagctcatcc tgggcggtac aatcttagtg
    27481 ctaaatggct gaaaagattg gttgcgcatt ttgccgaaca ctacggcgac gcgatggaag
    27541 tcgcgcagtg ccagcaatcg cccaatgaac gcacccagct ggcgacactt gcgcgtcagc
    27601 atcatttatg ggcatcacaa ggatctgatt ttcatcagcc atgcccgtgg atcgaactgg
    27661 gtcgtaaact ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc
    27721 agaacaccac agagagggaa ttatgagcca gtttttttat attcatcctg ataacccgca
    27781 gcaacgcctg atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga ttgtttatcc
    27841 aactgattcc ggctatgcgc tcggctgtaa aattgaagat aaaaacgcga tggagcgtat
    27901 ttgtcgaatt cgccagctgc cggacggtca caactttacc ctgatgtgtc gcgatctttc
    27961 tgagctctcg acctattcat tcgtggataa cgttgcgttt cgtttgatga aaaacaacac
    28021 gccgggcaac tacaccttca tcctgaaagg gacgaaggag gtgccacgcc gtctgttgca
    28081 ggaaaaacgc aaaaccatcg gtatgcgtgt gccttctaac ccgattgcgc aggcgctgct
    28141 tgaagcattg ggcgaaccga tgctttccac atcactgatg ctgccaggca gcgaatttac
    28201 cgaatccgat ccggaagaaa ttaaagaccg tctggaaaaa caggtggact tgattattca
    28261 cggcggctat ctcgggcaga aaccaacaac ggttattgat cttactgatg atacgccggt
    28321 cgtggtgcgt gaaggcgtag gtgatgtgaa gcctttctta taacaaattg cgttccacgg
    28381 atggaagact atggcaggga tatatcgctg cattttgctg ttgatagttg ggcttttttt
    28441 ctcttcgttg agttatccga aaaacacgga gatccctttt tatgaagaag ggatctcgct
    28501 ctttgatgtt gaagccactc tgcaaccgga tggggtgctc gacatcaaag aaaatattca
    28561 ttttcaggcg cgaaatcagc agattaagca cggattttat cgtgatttac cacgactctg
    28621 gatgcagcct gatggggacg ctgcactgct gaactatcat attgttggcg tcatccgtga
    28681 tggtattcct gaaccctggc atcttgactg gcatatcggg ttaatgagta ttgtcgtggg
    28741 cgataaacaa cgtttcttgc ctcaaggcga ctatcattat caaattcatt atcaggttaa
    28801 aaatgctatc ctgcgtgagg gggattctga tctgctaatc tggaacgtga ccggtaacca
    28861 ctggccgttt gaaatttata agaccctatt ttcactcaag ttgcctgata ttgcgggtaa
    28921 tccatttagc gaaatcgatc tctttactgg agaagagggc gacacatatc gaaatggccg
    28981 catccttgag gacggaagaa ttgaatctcg cgatccgttt tatcgtgaag atttcacggt
    29041 cctctaccgc tggcctcacg cattacttgg taatgccccg gctccacaaa cgacgaatat
    29101 tttcagtcat cttcttttac cctcaacgtc atcgttgtta atttgctttc cgagtctgtt
    29161 cctggcgtgt ggatggttat atctctggaa gcgcaggccg caatttacgc cggtagatgt
    29221 gattgaaacc gatgtcattc cgccagatta cacgcccggc atgttacgtc ttgatgcgaa
    29281 actggtttac gacgataaag gtttttgcgc cgatatcgta aatctgattg tgaaagaaaa
    29341 aattcatctg gaagatcatt atgacaagaa ccagcaaatc ctgattcgtg ttaatgaagg
    29401 cgcgaccaga aataatgcgg tattactgcc cgcagagcag ttattactgg aagcgttatt
    29461 tcgtaaaggc gataaggtcg ttcttacggg gagacgcaac agagtcttac gcaaggcatt
    29521 tttacggatg cagaaatttt atctgccgcg taaaaagtct tcgttttacc gacctgatgc
    29581 gtttttgcaa tggggcggaa tggcaatatt ggcggtcatt ctctacggta acctgagtcc
    29641 cgtaggctgg gaaggaatga gtctggtggg cgatatgttt attatgatct gctggcttct
    29701 tcctttttta ttttgttccc ttgagctttt gtttgcccgc gatgatgaca agccttgcgt
    29761 taatcgtgtc atcatcactt tgtttttacc actgatttgt tcaggtgtgg ctttttactc
    29821 tctctacatc aatgtcggag atgtattctt ttactggtat atgccagcgg gttattttag
    29881 cgctgttttc ctgaccggtt atctcactgg catggggtat atttttctgc caaagtttac
    29941 ccaaactggg cagcaacgtt atgcccacgg tgaagctatc gttaactatc ttgcgcgtaa
    30001 agaggcagca acacacagtg ggcggcgtag gaaaggggaa acacggaaac tggattacgc
    30061 tttgttaggt tgggctatat cggcaaattt ggggagggaa tgggcgttac gcatcgcccc
    30121 ttcgctttct tcggcgattt gcgctccaga gattgcccgt aacggcgttt tattctcatt
    30181 acagacgcac ctgagttgcg gggcctatac cagtttgttg gggcggagct attctggtgg
    30241 tggttctggc gctggtggcg gaggcggtgg tggctggtaa tcaagctgat gttaatcgaa
    30301 acaagcttta accataaaga tagtgtagta tgttgcgcct caaagccagg caagttaaaa
    30361 cgtcgagtcg tttacttaag gcctgaagag ttcaaacaga ctttactatc agtgagtcag
    30421 atgtaaattc tgactcactg ttgcacaaaa gcatatcaga cgcctgggaa ggcgacacca
    30481 aaaggaagct ctatgagcga aaagctacag aaagtgctgg cgcgtgccgg ccacggttct
    30541 cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct
    30601 aaactcggcg atcgcgttga aattacccct ggattgaaaa tccgtatcga tggtcacctg
    30661 atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa
    30721 ggtgagttgt gtacccgtaa cgatccggaa gggcgtccaa cggtgtttga ccgtctgcca
    30781 aagctgcgtg gcgcacgctg gattgccgta ggtcgcctgg acgttaatac ctgtggtctg
    30841 ctgctgttca ctaccgacgg tgaactggca aaccgtttaa tgcatccaag ccgtgaagtt
    30901 gaacgtgaat acgccgtgcg cgtatttggt caggttgacg acgcgaaact gcgtgatttg
    30961 agtcgtggcg tgcagctgga agatggtccg gcagccttta aaaccatcaa gttcagcggc
    31021 ggcgaaggga tcaaccagtg gtacaacgtt actctgaccg aaggccgtaa ccgtgaagtt
    31081 cgtcgtctgt gggaagcggt tggtgtgcag gtaagccgcc tgatccgtgt tcgttacggt
    31141 gatatcccac tgccgaaagg tctgccacgc ggtggctgga cggagctgga cctcgcccag
    31201 actaactatc tgcgcgaact ggtggagcta ccgccagaaa ccagctctaa agtcgctgta
    31261 gaaaaagacc gtcgtcgtat gaaggcgaat cagattcgtc gtgcggtgaa acgtcactgt
    31321 caggtgagcg gcggtcgtcg ttctggtgga cgtaataata acggttaatt taagttaagt
    31381 ataaaataaa ggccctgcgg ggccttttct tcatttgaag tatgataaat agtcgcagta
    31441 tttta
//
LOCUS       DAZGTJ010000057        30609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000057 DAZGTJ010000000
VERSION     DAZGTJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.057"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            106..1902
                     /locus_tag="ACHK8E_003136"
     CDS             106..1902
                     /locus_tag="ACHK8E_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFP0345367.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1920..2588
                     /locus_tag="ACHK8E_003137"
     CDS             1920..2588
                     /locus_tag="ACHK8E_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFP0345368.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2603..3274
                     /gene="dsbI"
                     /locus_tag="ACHK8E_003138"
     CDS             2603..3274
                     /gene="dsbI"
                     /locus_tag="ACHK8E_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFP0345369.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3320..4108)
                     /gene="ygiD"
                     /locus_tag="ACHK8E_003139"
     CDS             complement(3320..4108)
                     /gene="ygiD"
                     /locus_tag="ACHK8E_003139"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP0345370.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4251..5024
                     /gene="zupT"
                     /locus_tag="ACHK8E_003140"
     CDS             4251..5024
                     /gene="zupT"
                     /locus_tag="ACHK8E_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP0345371.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5082..5252)
                     /gene="yqiD"
                     /locus_tag="ACHK8E_003141"
     CDS             complement(5082..5252)
                     /gene="yqiD"
                     /locus_tag="ACHK8E_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP0345372.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5514..6167)
                     /gene="ribB"
                     /locus_tag="ACHK8E_003142"
     CDS             complement(5514..6167)
                     /gene="ribB"
                     /locus_tag="ACHK8E_003142"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP0345373.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6271..6419)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6541..6840
                     /gene="ubiK"
                     /locus_tag="ACHK8E_003143"
     CDS             6541..6840
                     /gene="ubiK"
                     /locus_tag="ACHK8E_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP0345374.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6875..7075)
                     /gene="glgS"
                     /locus_tag="ACHK8E_003144"
     CDS             complement(6875..7075)
                     /gene="glgS"
                     /locus_tag="ACHK8E_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0345375.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7344..7973
                     /gene="yqiJ"
                     /locus_tag="ACHK8E_003145"
     CDS             7344..7973
                     /gene="yqiJ"
                     /locus_tag="ACHK8E_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0345376.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            8000..9661
                     /gene="yqiK"
                     /locus_tag="ACHK8E_003146"
     CDS             8000..9661
                     /gene="yqiK"
                     /locus_tag="ACHK8E_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0345377.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9901..9960
                     /locus_tag="ACHK8E_003147"
     CDS             9901..9960
                     /locus_tag="ACHK8E_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0345378.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(10080..11513)
                     /gene="hldE"
                     /locus_tag="ACHK8E_003148"
     CDS             complement(10080..11513)
                     /gene="hldE"
                     /locus_tag="ACHK8E_003148"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP0345379.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11561..14401)
                     /gene="glnE"
                     /locus_tag="ACHK8E_003149"
     CDS             complement(11561..14401)
                     /gene="glnE"
                     /locus_tag="ACHK8E_003149"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP0345380.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14424..15725)
                     /gene="ygiF"
                     /locus_tag="ACHK8E_003150"
     CDS             complement(14424..15725)
                     /gene="ygiF"
                     /locus_tag="ACHK8E_003150"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP0345381.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15967..16587
                     /gene="ygiM"
                     /locus_tag="ACHK8E_003151"
     CDS             15967..16587
                     /gene="ygiM"
                     /locus_tag="ACHK8E_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP0345382.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16651..17889
                     /gene="cca"
                     /locus_tag="ACHK8E_003152"
     CDS             16651..17889
                     /gene="cca"
                     /locus_tag="ACHK8E_003152"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP0345383.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(18077..18898)
                     /gene="bacA"
                     /locus_tag="ACHK8E_003153"
     CDS             complement(18077..18898)
                     /gene="bacA"
                     /locus_tag="ACHK8E_003153"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0345384.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(18989..19357)
                     /gene="folB"
                     /locus_tag="ACHK8E_003154"
     CDS             complement(18989..19357)
                     /gene="folB"
                     /locus_tag="ACHK8E_003154"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP0345385.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19462..20079
                     /gene="plsY"
                     /locus_tag="ACHK8E_003155"
     CDS             19462..20079
                     /gene="plsY"
                     /locus_tag="ACHK8E_003155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP0345386.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(20092..21024)
                     /gene="ttdR"
                     /locus_tag="ACHK8E_003156"
                     /gene_synonym="dan"
     CDS             complement(20092..21024)
                     /gene="ttdR"
                     /locus_tag="ACHK8E_003156"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP0345387.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            21231..22142
                     /gene="ttdA"
                     /locus_tag="ACHK8E_003157"
     CDS             21231..22142
                     /gene="ttdA"
                     /locus_tag="ACHK8E_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP0345388.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            22139..22744
                     /gene="ttdB"
                     /locus_tag="ACHK8E_003158"
     CDS             22139..22744
                     /gene="ttdB"
                     /locus_tag="ACHK8E_003158"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP0345389.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22793..24256
                     /gene="ttdT"
                     /locus_tag="ACHK8E_003159"
     CDS             22793..24256
                     /gene="ttdT"
                     /locus_tag="ACHK8E_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP0345390.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(24299..25312)
                     /gene="tsaD"
                     /locus_tag="ACHK8E_003160"
     CDS             complement(24299..25312)
                     /gene="tsaD"
                     /locus_tag="ACHK8E_003160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP0345391.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25550..25765
                     /gene="rpsU"
                     /locus_tag="ACHK8E_003161"
     CDS             25550..25765
                     /gene="rpsU"
                     /locus_tag="ACHK8E_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP0345392.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25876..27621
                     /gene="dnaG"
                     /locus_tag="ACHK8E_003162"
     CDS             25876..27621
                     /gene="dnaG"
                     /locus_tag="ACHK8E_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0345393.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27816..29657
                     /gene="rpoD"
                     /locus_tag="ACHK8E_003163"
     CDS             27816..29657
                     /gene="rpoD"
                     /locus_tag="ACHK8E_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP0345394.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            29870..>30609
                     /locus_tag="ACHK8E_003164"
     CDS             29870..>30609
                     /locus_tag="ACHK8E_003164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345395.1"
                     /translation="MFFLCVGKNKGWRKVQKASDYGVIILTEEQFINFIDTGEIPLEQ
                     DCVVENDISKSLEKDVNTYKSMIDEFSLSIRTVREFRRSNALIAVFVDGYAAGWRFAV
                     KECYKDSLDIKLTPVKTEFFNYEIWTQGDSFQFNRGDVFYSDRLAYDSYCDFLKLDDA
                     VVLQVKYECFSGYDSVSTFDGVMDGEFSFSRMEPRPVHKLPVLIETQLYDPGKIIVDV
                     FVPDAAKKQLKLKDTLELKQDEFVCLLQS"
BASE COUNT         7612 a         8071 c         7634 g         7292 t
ORIGIN      
        1 tttattgaat taatatttat attcacgtaa cgaatttatt agcaccctct caacagtcga
       61 tattcatcat tattatatat ttgcgttata taaggagata tatccatgtt tgataaatat
      121 agaaaaacac tcgtagccgg aactgtggcg ataaccctgg gtttgtcagc atcgggggtg
      181 atggctgcgg gttttaaacc agcgccgcct gccgggcaac tgggtgcggt cattgtcgat
      241 ccctacggca atgcaccact gaccgctttg gttgacttag atagccatgt tatttctgac
      301 gtcaaagtta ccgtccatgg gaagggcgaa aaaggtgtag aaatcagcta tcccgtgggt
      361 caggaatcac taaaaactta cgatggtgta ccgatttttg gtctttatca gaaatttgct
      421 aacaaagtga ccgttgagtg gaaagaaaac ggcaaggtca tgaaagatga ttatgtggtg
      481 cacacttcgg ccatcgtcaa taattacatg gataaccgct ctatatccga tttacaacag
      541 accaaagtta ttaaagtcgc accgggtttt gaagatcgcc tctatctggt taatacccac
      601 acctttaccg cccaaggttc cgatctccac tggcatggtg agaaagataa aaatgccggt
      661 atccttgatg cgggtccggc aaccggcgca ctaccttttg atatcgcgcc ctttactttc
      721 atcgtcgata ccgaaggtga ataccgctgg tggctggatc aagacacttt ctacgatggt
      781 cgtgaccgcg atatcaacaa gcgtggttat ctgatgggta tccgcgaaac gccacgcggc
      841 acctttaccg ctgtacaagg tcagcactgg tacgagttcg acatgatggg gcaggtgctc
      901 gaagatcaca aactaccgcg cggatttgct gacgccactc atgagtccat tgagacgcca
      961 aatggcacgg tactgttgcg cgtaggtaag agtaactatc gtcgcgatga cggcgtacac
     1021 gtcaccacca ttcgtgacca tatcctcgaa gttgataaat ctggtcgcgt tgtcgatgta
     1081 tgggatctga ccaagatcct cgatccgaaa cgcgatgcac tgctcggcgc gctggatgca
     1141 ggtgcagttt gcgttaacgt tgaccttgcc catgcaggac aacaggcaaa actggaacca
     1201 gatacaccgt tcggcgacgc gctgggtgta gggccaggcc gcaactgggc gcacgtgaac
     1261 tctatcgcct atgacgcaaa agatgactct atcatcctct cctctcgcca ccagggtgtt
     1321 gtgaaaattg gtcgtgataa gcaagtgaaa tggatccttg cgccctctaa aggttgggaa
     1381 aaaccgctgg ccagcaagct gctgaaaccg gttgatgcta acggtaagcc aattacctgt
     1441 aacgaaaatg gcctgtgcga aaactcagac ttcgacttta cctacaccca gcataccgcc
     1501 tggatttcca gcaaaggaac gctcaccatt tttgataatg gcgatgggcg tcatctggaa
     1561 caacctgcct taccaaccat gaaatactcc cgctttgtgg aatataagat tgatgagaag
     1621 aaaggcaccg ttcagcaagt gtgggaatac ggtaaagaac gtggctacga tttctatagc
     1681 ccgattacct ccatcattga atatcaagcc gaccgtaaca ccatgtttgg cttcggtggt
     1741 tctattcatt tgttcgatgt cgggcagcca accgtcggta agttgaacga aatcgattac
     1801 aaaaccaaag aagtgaaagt ggaaatcgac gtgctgtcag ataaacccaa tcagactcac
     1861 tatcgtgcac tgttagtccg tccacaacag atgttcaaat aattaagaag ggaataaaaa
     1921 tgtcgaaatt agggatctca tcactattta aaaccatact gcttaccgca gctttggctg
     1981 tttcatttac cgcttctgca tttaccgaag gcacggatta catggttctg gaaaaaccta
     2041 ttccgaatgc cgacaaaacg ctgattaagg tattcagcta cgcctgcccg ttctgttaca
     2101 agtacgacaa agcggttacc ggtccggtat cggaaaaagt gaaagatatc gttgctttca
     2161 cgccgttcca tctggaaacc aaaggtgaat acggcaaaca agcaagcgaa gtctttgcgg
     2221 tcttgatcaa taaagataaa gcagcaggca tttccttatt tgatgctaac tctcagttta
     2281 agaaagccaa gtttgcctac tacgcggctt atcacgacaa aaaagaacgc tggtctgacg
     2341 gtaaagatcc ggctgctttt atcaaaaccg gtctggatgc cgcaggtatg agcttggcgg
     2401 attttgaagc tgcactgaaa gaaccagcgg ttcaagaaac actggaaaaa tggaaagcct
     2461 cttatgatgt cgccaaaatc caaggggttc cagcctatgt cgttaatggt aagtacttga
     2521 tctacaccaa gagcatcaaa tccatcgacg cgatggcaga ccttatccgt gagttagcaa
     2581 gcaaataaca gggaaacagg ctatgggtat taagggaatg tggaaagacc ttcgcacatc
     2641 accagttgac acactggtca gatggcagga gcaacgcttg ttgtggttgt taatggccgt
     2701 tgcgatgggc gcgttaatta tcctggcgca ctctttcttc cagatctatc tctacatggc
     2761 accctgtgag caatgtgtat acattcgcta cgccatgttt gtgatggtta ttggaggatt
     2821 ggttgcagcg atcaacccaa agaacatcat cctaaagcta attggctgcg tgatggcatt
     2881 ttacggcagc attttgggac tgaagttttc gctcaaacta aacgatatcc accatgcagt
     2941 acataatccg gatcccgatt cactgtttgg cgtacagggt tgctctaccg atccaacttt
     3001 tccctttaac ctgccgctgg cacagtgggc accaaattgg ttcaagccta caggggattg
     3061 tggctatgac gcaccaatcg ttcctgatgg cgtcacactg agcagcacgc aacagtggtt
     3121 tgtggaaatg tatcagcaat ctgaaggctg gtatctgcta ccaccatggc attttatgaa
     3181 tatggctcag gcttgtatgc tggcttttgg tatgtgtctg gtgctgctgg tcattatgag
     3241 tggcgcatgg gcgcttaaaa tcgtcagagg ctaactcagc aacacccgaa gccagtcgtt
     3301 ctggcttcgg gttcttttat tagcctatct gcaccgacaa catacttaaa ctgcccattt
     3361 ctataccatc gaccggaatg gtaattggct cctgcccatc ccacgcacct aagacataca
     3421 acaacggcag atagtgctct ggcgttgggt tcgataacgc gccaccttca tggtcgagat
     3481 aattcaccag aggatgctgc tctaccggcc cttgccacgt cagattcgct ttcacatact
     3541 cattaaacga cgtcgcccac ggatacggtg aactatcacc gtgccacttc actgtgcgca
     3601 ggttatgcac cacgttaccg ctggcgacca acattattcc ttcatctcgc agcgccgcga
     3661 gtttgcgccc catttcgaaa tgccaggcgg caggtttgct actgtcgata ctcaactgca
     3721 ccatcgggat atccgcgtca ggatacatct taatcagcac gccccaggaa ccgtggtcaa
     3781 agccccaggc ttctttatcc agcgtcaccg ggatcggcgc taacagctca accagacgct
     3841 gtgccagcgc aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc
     3901 caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa
     3961 accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc cccaacgtct
     4021 gccagctgcg ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa
     4081 acaatgctgg catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga
     4141 tatcctcaca atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat
     4201 cagttatttt gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac
     4261 ctctcattct gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc
     4321 tcgggcaaaa accctcgaac cgcttactgg cgttttcgct aggatttgcg gcggggatca
     4381 tgttgctcat ctcattaatg gaaatgcttc ctgccgcact agccgccgaa ggaatgtcgc
     4441 ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg ctattttggc ctggaccgca
     4501 tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg ttgccaaaat
     4561 cgatcaagcg cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag
     4621 ggattgccac ctttgtcacg gcgagcagca acctggagct gggctttggc atcgcactgg
     4681 ccgttgcgtt acacaatatt cctgaaggtc tggcggttgc aggcccggtt tacgcggcaa
     4741 cggggtctaa acgcaccgca attctttggg cggggatttc tggactggca gaaattcttg
     4801 gtggtgtgct ggcgtggtta atcctcggta gcatgatttc cccggtggtc atggcggcaa
     4861 tcatggcggc ggttgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg
     4921 ccaaagagat tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga
     4981 tgggattcag tttagtgctg ctacaaacgg cgggaattgg ttaaaagcaa catcagaagt
     5041 tgtccagcgc atggacaact tctgccagct tcactaaagc cttattctac cttttcggct
     5101 gtctcttctc tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc
     5161 tggcgaaacg cccgacaata acgtttcaaa tgcaggaaat aacccaccac aacaagagca
     5221 atcaatacaa tccagtacca ggcgataaac atccttttac ccttaatatt taacaatact
     5281 aaacatttag cgcataaatt tcacatatcc tttttcggat atattcatat ggtcgcataa
     5341 taacggaaca attatatgat tttgatcata atgaaaaaga atgcctgata tcaatgaaat
     5401 tttctcatag aaagagaagc ggaattaaca tcttgcatag cagggcaaaa taaaaaccga
     5461 agccagctac aacttcggtt tcttgattaa gacagtaaat taagcagcga ttttcagctg
     5521 gctttgcgtt catgcgcctg acgataagcc accaggtctt caatagtcac gagcgccata
     5581 ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta
     5641 gtcagctcac acagcacacc agccggttta aagccagcca gcgtcatcag atcaatagtc
     5701 gcttcagtat ggccgccacg cgtcagtaca ccaccagcct gagcgcgaag tgggaaaacg
     5761 tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc
     5821 gtaatacggt cagcggcaga aacaccggta gtcacacctt cagctgcttc aatggtcacg
     5881 gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag atcgagttgt
     5941 ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg aatggtcagc
     6001 gccatctgct caacggtcat ggtttctgcc gggaagatca tatcaccttc gttttcacgg
     6061 tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcaaccag tgcattttca
     6121 acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct
     6181 cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa
     6241 taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg
     6301 tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg
     6361 gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg
     6421 gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt
     6481 atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag ggaaactaca
     6541 atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa
     6601 ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagcgcag
     6661 ctgacgcgtc tcgatctggt aagccgcgaa gaattcgacg ttcagacgca agtcctgtta
     6721 cggactcgtg aaaaactggc gttactggag cagcgcatca gtgaactgga agcgcgcaat
     6781 aattcggtag cagatcttca atctcctccg gctatcccgc ctattgataa agcagaataa
     6841 agcatcacaa aaacgggcca gacggcccgt aatatcagtg ttctaattca agttctctgg
     6901 tttgcatcat ctgttggcaa taccaggcat aacgtgcgca aaaccaggtt ctatgttctt
     6961 catccatgtt accagtaacg gccagaatat cgaccgggcg accttctgcg tgcattctgg
     7021 caaaactacg cgccaggaaa tcgaaattat ttaaagaatt aagactatga tccataagca
     7081 catcctccgg ttatcgcata ccttttaagg ccatatgctt tttctgattt cagtattccc
     7141 caatcagtgt agtgccttta aacataacgt gcgtaaatat attgtccccg atcacacttt
     7201 ttagtgaaaa ataaaaaacc atattaaaag actttctctt ccagaacttc tctaacatgt
     7261 gttattatta acaaagataa atgtaagcaa ggaaactatc ctaaagtaaa tattgcgtca
     7321 ctaaatggac atcggagtga catatgattt tattcgccga ctataacacc ccttatcttt
     7381 tcgctatttc ctttgtactg ttgatcggcc ttttagaaat ttttgcgctt atctgcggtc
     7441 acatgctctc cggtgctctt gatgcacatc ttgaccatta tgattccata accacaggtc
     7501 atattagcca ggcacttcat tatctcaata tcggaagatt gccagctctc gtcgttctct
     7561 gcttactggc tggttttttt ggtcttattg gcatcctgtt gcagcatacc tgcctcatgg
     7621 tctggcaatc accactctcg aacctgtttg tggttcctgt cagtttactg tttacgatta
     7681 ttgcggtgca ttataccggt aaaatagttg cgccctggat tccacgcgat cacagctcag
     7741 ctattacaga agaagaatat attggtagca tggcgttaat taccggtcat caagccactt
     7801 caggtaatcc ctgcgaagga aaactcaccg atcaatttgg ccaaatacat tatttactac
     7861 tggagcctga agaaggaaaa attttcacta aaggagacaa agtgttaatt atttgccgac
     7921 tttctgcaac tcgatatttg gcggaaaaca atccctggcc ccaaatattg taatttatta
     7981 atcaaaagga aatttaataa tggatgatat tgttaattct gtgccctcct ggatgtttac
     8041 cgcgattatt gccgtatgca ttctgtttat tattggaatt attttcgcca ggctctatcg
     8101 tcgcgcttcg gcagagcaag cttttgttcg tactggttta ggtgggcaaa aagtggtaat
     8161 gagcggtggc gcaatcgtga tgccgatctt tcatgaaata atccccatca atatgaatac
     8221 tctgaagctg gaagtcagcc gctcaaccat tgatagcctg attacgaaag atcgtatgcg
     8281 cgtcgatgtc gttgtcgctt tctttgtgcg ggtaaaaccg tcagtggaag gtattgccac
     8341 cgccgcccag acgcttgggc aacgcaccct gtcgcctgaa gacttacgta tgttggttga
     8401 agataaattt gtcgatgccc tccgtgcaac agctgcacaa atgaccatgc atgagttaca
     8461 ggatacccgc gagaacttcg tgcagggcgt acaaaatacc gtagctgaag atctgtcgaa
     8521 aaacggcctg gaactggaaa gcgtttcact taccaacttt aaccagacgt cgaaagaaca
     8581 tttcaatcct aacaacgcct ttgacgccga aggtttaact aagctgacgc aggagacgga
     8641 gcgccgtcgt cgcgaacgta acgaagttga acaggatgta gaagttgcgg tgcgtgagaa
     8701 aaaccgtgat gcgctttcgc ggaagctgga gattgaacag caagaagcgt ttatgacgct
     8761 tgagcaggag cagcaggtta aaacccgtac cgctgagcag aatgcgaaaa ttgcggcttt
     8821 tgaggccgag cgtcgtcggg aagcagagca gacgcgaatt ctggctgaac gacagattca
     8881 ggaaacggaa atcgaccgcg aacaggccgt ccgttccaga aaagttgaag ctgaacgtga
     8941 agttcgcatt aaagagatcg aacagcagca ggtcaccgaa atcgccaacc agacgaaatc
     9001 gatcgcgatt gccgccaaat cggaacagca gtcacaagcg gaagcgcgtg ccaaccttgc
     9061 acttgcagaa gcggtaagcg cccagcaaaa cgtagaaacc actcgccaga ctgccgaagc
     9121 cgatcgtgct aaacaagtgg cgttgatcgc cgcagcgcag gatgcagaaa ccaaagcggt
     9181 tgaactgacc gtgcgggcga aagcagaaaa agaagccgca gaaatgcagg cggcggctat
     9241 cgttgagtta gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag cacaacgtgc
     9301 gctgaacgat gctatcaacg tactttctga tgaacaaacc agccttaaat tcaaactggc
     9361 attattgcag gcgctgcctg cggtaattga gaaatccgtg gaaccaatga agtctatcga
     9421 cggcatcaaa attattcagg tcgatgggct gaatcgtggt ggcgctacgg gtgatgcaaa
     9481 cacgggtaat gtggggggcg gaaacctggc agaacaagcg ttatcagccg cactctctta
     9541 ccgtacacag gcaccgctga ttgactcctt gctcaatgaa attggcgttt caggcggctc
     9601 actggcggca ttgacttcat ccttaagctc aacaactccc gtcgaagaaa aagcagagta
     9661 aaccctcttc tatgaataat ccctggccca aaaagccggg gattttttat atctgcgttc
     9721 cgctaaaagg tgcaaatgat caagctgttg cagcattttg cgtgactgct cggggaaggc
     9781 aaaactgcct ctgggaaagc gttgcgcggg gtccggcgct catcaacaat cggggagtca
     9841 gcaaggggct gaaacgggaa agcccctccc gaggaagggg ccttgtataa ggaaagggtt
     9901 atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc agcttattaa
     9961 cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt taggttgaaa
    10021 aaaaatcagc gtcaataggc ccgcctgcta caaagcgaga tctgtgaacc gctttccggt
    10081 tagccttttt tatcctgttg gatcttcttg atgatgttgg tcgtcgagca accgtcttca
    10141 aagttgagca ccagcacttc accaccgttg gcccagactt ctttactccc ggcaatctct
    10201 tctggtttat agtcgccgcc tttcaccagt agatctggca agataccggc gatcaagcgc
    10261 tgcggcgtgt cctcttcaaa cgacaccacc cagtcgaccg cttccagtgc gcccagcaca
    10321 atcatacgct gttcgagctg gttaacgggg cgggaatccc ctttcagccg tttggtggag
    10381 gcatcgctgt tcacagcgac aatcaaacgg tcacccagtt tacgggcatt tgccagataa
    10441 gagacgtgcc cagcgtgcag gatgtcaaag acaccgttgg tcatcaccac tttttcaccg
    10501 cgtttacgcg ctgccgctac agcaagcttc agttcctctt cggtcatcac accaaagccg
    10561 gtatctgcac gtccacgtac agcgttttcc agctcgatcg gcgaaaccgt ggatgtcccc
    10621 agtttgccga ccaccacacc agccgccgca ttggcaaaga agcaggcttc ttccagtgaa
    10681 ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg tgtcgcccgc accggtaacg
    10741 tcatacactt cctgcgcctg ggttggcata tgcagtggcg ctttacccgg ttgcagcagc
    10801 gacataccct gttcggaacg ggtcactaac agagccgaga gttcgtaatc ggcaatcagt
    10861 ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt taccgacaac agcttcaaat
    10921 tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc gctcaaaatc ggtacctttt
    10981 ggatcaatca gcaccggaac acccgcttta cgcgccagtt ggatcatctg ctgtacgctt
    11041 gccagcgcac ctttggcgta atcagaaagc accagcgcgc caatcgaact cagtgcctga
    11101 ttaatccgct cgtgcagcgg ctgcggatca accccttcga aaccttcttc gaaatccaga
    11161 cggatcagtt gttggttgcg ggaaagtacc cgtaatttgg tgatggtcgg atgcgtcggt
    11221 acagaaacaa agtcgcattt gacgttgacg tcggccagag atttactgag cgcgcgcgcc
    11281 gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag caccgagaga agcgatattc
    11341 atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg tattcacttt aaccacgggc
    11401 accggcgctt ccggcgagat acggctggtg ggaccgtacc agtaacgatc cagcatcaca
    11461 tcaccaacca ccatcactcc tgcacgttca aactctggca gcgttacttt cattcctgtc
    11521 tcctgagaga ttcaaaattt gcgcgcgata ataccatact tcattcttcc accagccact
    11581 tctgccagct tgcccgcacc agttcacgct ctgcggtgaa gcaatcctcc gacacatgac
    11641 ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg cagcgtcgtg taagcacggg
    11701 tcagcgccat cgcttcctgc tcttccataa tgtcgttttg cgccagtagt tccagaatgc
    11761 gcacgttgtc tgaccagcgc gttaacttcg gtttttcgtg agcgtagcgc aacaccagat
    11821 attgagtaat aaattcgata tcggtaattc cgccttcatc ggctttgata tcaaagcgat
    11881 cgcgatgttt attgccgaga tgggcacgca ttttctcgcg catttcccgc acttctgttt
    11941 gcagggtttt gccttcgcgc ggcagcgtca taatctcccg gcgtactgcg tcaaagtgcg
    12001 cggtgagctg cggatcgccg tacactacac gcgcacgcac cagcgcctga tgctcccacg
    12061 tccaggcctc gtttttctga taatcagcaa atgcttctgc ggatgtcacc agcattcccg
    12121 ccgccccgga cggacgcagt cgtgcatcca cttcatacaa aatgccggaa gaggttcgcg
    12181 tactgaacag atgcataatg cgttgcgcca gacgcagata aaactgtcgc ccgtcgattt
    12241 cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg gaggaagatc aggtcaagat
    12301 cggaactgta gcctaactcc cagccaccca gcttgccgta gccgaccacc gcaaaaccac
    12361 gcccttcgcg ttcgttcagg tgatttggct taccgtagcg ggcaaccatt tgaacccacg
    12421 cctgctgaac gacggcatca atcatggctt ctgccagcca ggttaagtga tcgctcactt
    12481 tcattaccgg tagcgtaccg gcgatatccg ctgccgcaat gcgtaacagc tgcgcctgtt
    12541 tgaactgacg cagcgcctca agctgttgtt cttcgtcatc ttccggcacg cgcagcaaat
    12601 actggcgcag ttcatcacgg taggcatccg tcgccgttgg ttggtagagg gtatttggat
    12661 cgagcaattc atccagcaat aatggataac gcgctagctg gctggcaatc atcggcgatg
    12721 cagcacacag agaaatcaaa tgtttaagcg ccgcagggaa ttcactaagc agctcaagat
    12781 aagtggtgcg agtaacaatc cccaccagca aggcggtaat gcgcgacagc gtgacagcag
    12841 cgtcttcacg cgcacagaca tcacttagca gatgtggcat cagatggtcg agcacctgac
    12901 gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg gaaatcagca atcagcatta
    12961 gcacctgttt gcgatcatcc tcgctaagat gcgccagcac tggcgtggtg tcatcttcct
    13021 gcaacgcatc ctgccacagc tcacgccact gttccgacag cgactcttcc tgagtttcac
    13081 tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac attggccata tgtgcggtca
    13141 gcaccccggt caattgcggc cagtcagcaa agtccatcgc ccacgccagc cgcgcacggt
    13201 taagctcatc aaaaggaagc gtctgggttt gttcgtcgtt aatgctttgc agcaggtttt
    13261 ccagacgccg caggaacaga tacgccactc gcaattgttc agcatcgttt tcagaaagca
    13321 gatgcagcgc ggcaatggcg ctgagcgttg gcagtaaaga gcgcgattgc agcgacggtt
    13381 cgcgcccgcc gcgaatgagc tggaacacct ggacgataaa ttcaatttcg cgaatgccgc
    13441 ctgcgccaag tttgatgttg tcggtcaaac cgcgtcgacg cacttcacgg gcaatcattc
    13501 ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat gtaacgacgg aaaacaaacg
    13561 gacgcagcat cgcacgcaac tcgttagcat agacgccgtc gctatccccc ataatccgcg
    13621 ccttgaccat cgcgtaacgc tcccagtcgc gcccctgctc ctggtaataa tcttccagcg
    13681 ccgcaaaact cagcaccagc gggccacttt caccaaacgg acgcagccgc atatccacgc
    13741 gatagacgaa accatcctgc gttggttgat ccagcacttt aatcagccgc tgccccatgc
    13801 gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc ctgggtacaa ccatgttccg
    13861 gccaggcaaa aatcagatcg atatctgagg agaaattcag ttccccaccg cccagcttac
    13921 ccatgcctaa aatcagcagc ggttgcgctt cgccctgcgc attgcacggc gttccccatt
    13981 cgcggcagca ggcgtcatac agccagtcac gcgccgcaac aatcagcgtc tccgccagat
    14041 ggctgagctg ctgcaatatg ctctcttcag taaccagtgc cagcgtttgc gcccaggcga
    14101 tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg cattaacccg gcttcgtcac
    14161 tcacattact gagcgcctcc tgcaaccatg ctgcgtaatg ctgccattcg tcggcctgcg
    14221 gcggttggct ttccagttcc gtcagccact ctggatgcgc aattatgctg tcctgcagaa
    14281 aatcactgaa agtaagtact gacttcgcct gtgcgctaag tgattcctcg gctaaaggct
    14341 ctggcagccg ctcaacaacg gtctgccagt actgctgtaa cggtgaagag agcggcttca
    14401 taagcgattt tatccttgcc tgattaacgt tttccgctgt gcaaccagaa cggttcctga
    14461 ttgtttgcct cattacggaa atgttcaatt tcaatgcgtt gcccggtcgc aatagcgtga
    14521 cgcagcccct gccagttctc cagccaggct tgcgcgacga caggatcgta gtaacccgcc
    14581 agcaacagga ttgagtcaat atcacgcgtc aggcgtggca actggtcacg gtagcgatcg
    14641 cccaacggct ggcagaaaac gcttttcagt tcagcggcat ggcgggaaag atggatatcg
    14701 gcaaagcgtt tgaaggaatc gctcatttta ctctgcgctt tggcatctaa aaacggctgc
    14761 caggctttgc ttaccagcca ttcggtcaat gccagcttcg ccattgccgt ttcggtagag
    14821 tagaccgccg tcacggcaga aaccgctgaa gcgatggtcg cttcgcattg agtcaataaa
    14881 tcacgtaagt gagtgctcgc tttacgcggc acaatgccac cgaacagcat cagcgtatga
    14941 cggaccaggc caatggctgc cagcacctgc tctttcgccg catcattgcc gcgtacccat
    15001 agttcttcat gatactgcca ttgcgctaac gccagctcga gcgccgcttc cagcccctgt
    15061 tccacatcag cttttgccgc aacatgtaaa atggtggtcg gtttgatttc acgcgccgga
    15121 ttgccctgcg ccagatgata gccacgcgcc gctttgctca gactgccctg gcgtaatccg
    15181 gtttgcgata ccagttggtt cgccagtttc agcaccgcgc gcgtgtcgcc gctaagcagt
    15241 tccagttcca gctcgcagat aggttcagca aattcacccg ctttcacttc cccctggtcg
    15301 agggcgattt caatttggct accatcaacc tctaccagcc acttttcgcg ataaaaatcg
    15361 gtgctaaaca gcggctgcac gcgggaagcg agatcggtag gcaattcgcc gttcggccag
    15421 acttccgtcg gtaactgcgc caggtcgagc gtcggttcgc tcaacgccac gttatattcc
    15481 gggcgctggt gtaagccgcc tgtcactctt cctgcaactt tcatggtcat ctcatagcga
    15541 ccgttttcgc cacgaatacg caagcccatg tcgtgcccac gcagccagtt atccggcgtt
    15601 tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct cgccgcccag cgtattgaga
    15661 tggtcacgca acgcctcaac ggcactgtga ttaacaataa actttaattc gatttcctga
    15721 gccatagcct ttacttatcg gttatgtcac ataagtgacg atgaacggcg aacttaatgc
    15781 gatctttttt gtcagtagat agtattttgc gccaaattgc catgcaacgg gcaatttgac
    15841 gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga ggattccgat tgatgtcgaa
    15901 tgctttgcgt agcgacactg atgccactac tatcgttcca ctttccatga caataacgac
    15961 agcctgatgc caaaattacg cctgatcgga ttaactttac tcgcacttag cgccactgcc
    16021 gtctcacacg ctgaagaaac gcgctatgtt tctgatgaac tgaatacctg ggtccgtagc
    16081 ggtccgggag atcattatcg cctcgtgggc acggttaacg ccggcgagga agtgacctta
    16141 ttacaaactg acgccaacac caattatgcc caggtgaaag acagctctgg ccgtaccgcc
    16201 tggatcccgt tgaaacaact tagcacagag ccaagcctgc gctcccgtgt gccagatctg
    16261 gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata tcgataacac ctggaaccaa
    16321 cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca gcgtgatcaa cgggttaaaa
    16381 gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc agaaaaaggt cgatgccgcc
    16441 agcgtacagc tggatgacaa acagcgcacc atcatcatgc agtggtttat gtatggcggt
    16501 ggcgtgctgg ggcttggctt gctgcttggt ctggtactgc cgcacctgat cccaagccgc
    16561 aaacgcaaag atcgctggat gaactaaatc gccttcttcg ttgcactgtc ttattattag
    16621 gaattgtgta tttgacgaag ggatgatggc gtgaagattt atctggtcgg tggtgctgtt
    16681 cgggatgcat tgttagggct accggtcaaa gacagagatt gggtggtggt cggcagtacg
    16741 ccacaggaga tgctcgacgc gggctaccag caggtaggcc gcgattttcc tgtgtttctg
    16801 catccgcaaa cgcatgaaga gtatgcactg gcacgtaccg aacggaaatc tggttccggt
    16861 tacaccggtt ttacctgcta tgccgcaccg gatgtcacgc tggaagatga tcttaagcgt
    16921 cgcgatctga ccattaatgc gctggcccag gacgataacg gcgagattat cgacccgtac
    16981 aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg tttcccccgc ttttggcgaa
    17041 gatccgttac gcgtgttgcg cgtagcgcgt tttgctgcgc gttatgccca cctcagtttc
    17101 cgtattgccg atgaaactct ggcgctgatg cgcgagatga cccatgcggg tgaactggaa
    17161 cacctgacgc ctgaacgggt atggaaagag acggaaaacg ccctcaccac ccgcaatccc
    17221 caggtgttct tccaggtact gcgcgattgc ggcgcactgc gcgttttatt cccggaaatt
    17281 gacgccctgt ttggcgttcc ggcccctgcc aggtggcatc cggaaatcga cacgggtatt
    17341 cataccttaa tgacgctctc aatggcggcg atgctgagtc cgcaggtcga tgtccgtttc
    17401 gcaacgttat gccacgatct cggtaaagga ctgacgccac cagaactttg gccgcgtcat
    17461 catggtcatg gcccggcggg tgtgaagtta gtggaacaac tatgccagcg tctgcgcgtg
    17521 ccaaatgaaa ttcgcgattt agccagactg gtggctgagt ttcacgatct catccacacc
    17581 ttcccaatgc tgaacccaaa aaccatcgtc aaactatttg attccatcga cgcctggcgt
    17641 aaaccgcagc gtgtcgagca actggcgctg accagcgagg ctgatgtgcg cggcagaacc
    17701 ggttttgaat cagccgacta cccgcaaggc cgctggttgc gcgaagcctg ggaagtggca
    17761 cagtcagtgc cgacaaaagc cgtcgttgaa gcgggattta aaggtgtgga gattcgcgag
    17821 gagctgaccc gacggcggat tgcggcggtg gctggctgga aggaacagcg ttgcccaaaa
    17881 cctgagtgag tgttggatat tttgccggat gcgacgtaag cgtcttatcc ggctacatgt
    17941 caacgacagt tgtaggcctg ataagacgcg cgagcgtcgc atcaggcgtt gattgccgga
    18001 tgcggcgtga acgccttatc cggcctacgc aatctgataa gacgcgtcag catcatatcc
    18061 gacactgttc ttcacatcaa aagaacacaa cgtacaccgc agccgccaca ataaagcgat
    18121 aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta
    18181 tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggtag
    18241 tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggcaccg
    18301 ccagcaggaa cgagaactcg gaagcagcgt aacggctcac ccccatcagc atcccacctg
    18361 aaatggtcgc cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa
    18421 tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct
    18481 tcaggcattc ggcggcaatc agcaacagac cgccaacgac cagtgcatac atcacattta
    18541 tcgggttaaa caatgattta atcgtgtcgt ggaacaacag ccccagtacc accgccggaa
    18601 tcatccccag caaaatgtgg atcagcgtta aacggccttt gctttctcct tcgtgctgca
    18661 acgggcgacc aaagtgaata ccaataaggc caaacagacg tcgccagaac atgacaacca
    18721 ctgccagaat tgatcctaac tggatcacga cttcaaaggt tttcgccgtg tcgccctcaa
    18781 accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg
    18841 tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca
    18901 ataaacccct aaattataaa aatgtaacgc ttgtccgaac tactgcagta tgaccaggtt
    18961 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    19021 caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac
    19081 gcacccacgg cgagttgaag cgtgctaaca gcaactccgc cacctcttca gccacacgtt
    19141 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgactac cgtttctgca atgtcagcgt
    19201 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc cacgccattt
    19261 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    19321 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    19381 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    19441 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    19501 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    19561 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    19621 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg
    19681 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    19741 cacatctggc ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt
    19801 gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    19861 gtgctattga gcggatattc gtcgctgggg gcgattgtca gtgcactgat tgctccgttt
    19921 tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    19981 ctgctgcgcc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    20041 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga gctacataac
    20101 ctgatagcct tcgtcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    20161 gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc
    20221 gctttgtgca tactccggca atactcgcac cagtttgcca ctctccagaa acggcaacac
    20281 atcccactcg gaacgcaaca taatcccttt gccctccagc gcccattgca acactatctc
    20341 gccgctattg gaggaaagat gcccgcttac cttcaccgat tttttctcct gaccgttccc
    20401 caactcccat attccgtggg tcatatcgcg ctctttggtc accaggcagt catgacgact
    20461 taattcctgt aaggattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa
    20521 tattctttta ttttttgtca aaagatgcgc aatataataa tcaggaattg cgtcattaat
    20581 acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa
    20641 atgcacctgt aactcaggat aattgcgcat cagttcggta atggctggag caatatggct
    20701 gcgcccaaaa ccaaaactgc aaccaatgcg gatcatcccc tccgggcgcg ttttgatttg
    20761 cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca
    20821 acgttggcca ctttcagtca gcgccacgcc gcgagcggag cggttcagca gagttgttgc
    20881 cagcgtattt tcaaggattt ggatccgctt ggtgacaaat gcaggcgttt gcccaagcgt
    20941 cgccgctgca gcactaaaac tcccgctatg gacgatttcc accagtacct gtaagtcttt
    21001 ggctaaaggc cagctattta gcattgttaa atatccgcag ttttgataaa cgcagtgtat
    21061 ttcgcaaaac attaccattc cctgctctgg cgcaatattc gttcttattt tcgcgttgat
    21121 tcacgaactg ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg
    21181 tagtttgtgg cggtgaattt ttccagacaa atacataaac tggagttgcc atgatgagcg
    21241 aaagtaataa gcaacaggca gtgaataagt tgacggagat tgtcgctaac tttaccgcca
    21301 tgatttctac ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg
    21361 aaacatcgtc gatgggaaaa atcatctacc acacgatgtt cgataacatg caaaaagcga
    21421 tcgacctgaa tcgtcctgcc tgtcaggaca ccggcgaaat catgtttttt gttaaggtcg
    21481 gttcccgttt cccactgctt ggcgagctgc aaagcatcct caaacaagcc gtggaagagg
    21541 caaccgtcaa agcgccactg cgtcacaatg cggtagaaat ttttgacgaa gtgaacaccg
    21601 gcaaaaatac cggcagcggc gtaccgtggg tcacctggga tatcgtcccc gacggtgacg
    21661 atgcggaaat cgaagtttac atggcaggcg gcggctgcac actacctggt cgttcgaaag
    21721 tgttaatgcc gtcggaagga tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca
    21781 ccctcgccgt aaacgcctgt ccaccggtac tggtaggcgt gggcatcgcc acctcggtgg
    21841 aaaccgccgc tgtgctctca cgtaaagcca ttttacgccc aattggcagc cgccacccca
    21901 atccgaaagc ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctggggattg
    21961 gtccacaagg gctgactggc aacagttcag tgatgggcgt acatatcgaa tctgccgccc
    22021 gccatccgtc aaccatcggc gttgctgttt ctacaggctg ctgggcgcat cgtcgcggca
    22081 cactgctggt tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat
    22141 gaaaaagatc ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga
    22201 tgtgatctac ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gtcgcttgat
    22261 cgaactcaaa cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc
    22321 catcgtgcgc aaaaacggcg acaaatggga gatggtatcc gtcggcccga caaccagtat
    22381 gcgtatggaa agttttgaac gtgaatttat cgaacagacc ggcgtgaaac tggtggtcgg
    22441 caaaggcgga atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctgcatgt
    22501 gattttcccg gcaggctgcg cagtactggc ggcaacccag gtggaagaga ttgaagaagt
    22561 gcactggaca gaactcggga tgccggagtc gctgtgggtc tgccgggtca aagagttcgg
    22621 tccgctgatt gtttctattg atacccacgg caacaacctg atagccgaaa acaaaaagct
    22681 gttcgccgaa cgccgcgatc ccatcgtgga agagatttgc gagcacgtcc actacatcaa
    22741 ataaccctcc cggagaggct caccccctct cctttttcgc aggcataaca cgatgaaacc
    22801 ttccactgaa tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat
    22861 tccggttccc gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt
    22921 gatcgttgga ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc
    22981 cattatcgcc attctctcgc cgtggctgct gtttagcccg gagcagctcg cccagccagg
    23041 ttttaagttc accgccaaat ccctctcctg ggcggtttct ggtttttcta attcggttat
    23101 ctggctgatt ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg
    23161 ccgcattgcg ctgattctgg tgaaaaagat gggacatcgc acgctgtttc tcggctatgc
    23221 ggtgatgttc tccgagctga tcctggcacc tgtaacaccg tccaactcgg cgcgcggtgc
    23281 ggggattatc tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga
    23341 cagcagttcg cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt
    23401 gaccagcgcc attttcctga cggcgatggc acctaacttg ctgttaattg gcctgatgaa
    23461 aagcgcatct cacgccacac tgagttgggg cgactggttc ctcgggatgt tgccgctcag
    23521 tattttactg gttctgctgg ttccctggct ggcttacgtg ctgtacccgc cggtactgaa
    23581 gtctggtgat caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg
    23641 ttcgcgtgaa aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg
    23701 cggtgattat atcgatgccg cgatggtcgg ttacagcgtg gtggcgctga tgctgcttct
    23761 gcgcattatc agttgggacg acattgtcag taataaagcc gcgtggaacg ttttcttctg
    23821 gctggcctcg cttatcaccc tcgctaccgg actcaacaac accggtttta ttagctggtt
    23881 tggcaaactg ttagcaggca gcttaagcgg ttattcgcca acgatagtga tggtggcgtt
    23941 gattgtggtg ttttatctac tgcgctactt tttcgccagc gccacggcgt atacctccgc
    24001 tctcgcgccg atgatgattg ccgccgcgct ggcgatgccg gaaatcccgc tgccggtgtt
    24061 ctgcctgatg gttggcgcgg caattggtct ggggagcatt cttacgccat acgccaccgg
    24121 acccagtccg atttactacg gtagtggtta tctgccaacg gtggattact ggcgactggg
    24181 ggcaattttt gggctgatat tcctcgtgtt gctggtgatt accggcttac tgtggatgcc
    24241 cgtggtgttg ctttaagtga ggacggtgcc ggaagtgact ctccggcacc taagagtttt
    24301 acgcggccgg taactctgcc agcggccagc gcggacgcac gctaacgcca agatccgccg
    24361 tcgcgcctgc tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac
    24421 aaaattccgg acgcgcgtag aacacttcgc cacggcgttt tttcatcatt tccgccagct
    24481 tcgcccgtaa cgtgcggtta gcactcacgc cacctgccat taccagtcgc ttaaagcccg
    24541 tctgatccag cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg
    24601 cgcgggcgat atcagcacgc gtctggtcgt cggtgccgtt atcacgaatg gtatttgccg
    24661 cgaaggtttt cagaccagaa aagctgaaat ccagccctgg acggtcggtc atcgggcgcg
    24721 ggaagacaaa gcgcccggct gtaccctgag ccgccatttt cgacagtaat ggcccgccag
    24781 gataatccag ccccagcagc ttcgcggttt tatcaaacgc ttcaccagcc gcatcatcga
    24841 tagactcgcc gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac
    24901 cgccggaaac cagcagcgcc acaaacggaa attccggtgg gttatcttcc agcatcggcg
    24961 ctaacagatg cccttccata tggtgtacag ggatcgccgg aacgttccag gcaaacgcca
    25021 gagaacgccc cacggtcgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat
    25081 aggccacagc atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca
    25141 acggtacggt tttacgcaca tgatcgcggg aggccagctc aggcactacg ccgccgtagt
    25201 cagcgtgcaa tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt
    25261 aaatggcgat gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt
    25321 ttacctcgct ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc
    25381 gcccctggag aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg
    25441 ccgcggtgct ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag
    25501 ctggcgttga tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat
    25561 taaagtacgt gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga
    25621 aaaagcaggt gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga
    25681 acgtaagcgc gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa
    25741 cgcacgccgc actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt
    25801 tgtagttgta aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc
    25861 taaaaatcgg ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca
    25921 cgcactgaca tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat
    25981 ttccacgcgt gttgtccatt ccacaacgag aaaaccccgt cattcaccgt taacggtgag
    26041 aaacagtttt accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg
    26101 aactacgaca agcttgagtt cgtcgaaacg gtcgaagagc tggcggcaat gcacaatctt
    26161 gaagtgccat ttgaagcagg tagcggcccc agccagatcg agcgccatca acgacaaacg
    26221 ctttatcagt tgatggacgg tctgaatacg ttttaccaac aatctttaca gcaacctgtt
    26281 gccacgtctg cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgcccgc
    26341 tttgcgattg gttttgcccc ccctggctgg gacaacgtcc tgaagcggtt tggcggcaat
    26401 ccagaaaatc gccagtcatt gattgatgcg ggcatgttgg tcactaacga tcaggggcgc
    26461 agttacgacc gtttccgcga gcgggtgatg ttccccattc gcgataaacg cggtcgggtg
    26521 attggttttg gcgggcgtgt gctgggcaac gatacaccca aatacctgaa ctcgccggaa
    26581 acggacattt tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac
    26641 gctgaaccca atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa
    26701 tacggcatta attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa
    26761 ctgttgttcc gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgc
    26821 gatgccgcct ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg tcgtcagcta
    26881 cgctttatgt ttttgcctga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa
    26941 caagcgtttg aagcgcggat ggagcaggcg atgccactct ccgcatttct gtttaacagt
    27001 ctgatgccgc aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca
    27061 ctgccactga tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta
    27121 ggcaacaaat taggcatact tgatgacaac cagcttgaac gattaatgcc aaaagcggca
    27181 gagagcggcg tttctcgccc tgttccacag ctaaaacgca cgaccatgcg tatacttata
    27241 gggttgctgg tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat
    27301 gaaaataagc tccctggact tggcttattc agagaactgg tcaacacttg tctctctcag
    27361 ccaggtctga ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc
    27421 cttgaaaaac tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc
    27481 accgactcac tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc
    27541 gctcgtgagc gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac
    27601 caggagctgg cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc
    27661 cgacagccgc actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg
    27721 cggtaaacaa ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa
    27781 caccaacctc atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc
    27841 tgaaacttct tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg
    27901 accatctgcc ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca
    27961 acgacatggg cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg
    28021 aaaacaccgc ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat
    28081 ctgaaatcgg gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg
    28141 aactgttgac ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc
    28201 aggttcaatg ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg
    28261 atcgtgttga agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga
    28321 acgcagaaga agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag
    28381 atctggacga tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg
    28441 acaacagcat cgacccggaa ctggctcgcg aaaaatttgc ggaactgcgc gctcagtacg
    28501 ttgtaacgcg tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga
    28561 tcctgaaact gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc
    28621 tggtcaacag catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga
    28681 agctctgcgt tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca
    28741 acgaaaccag cgatacctgg ttcaacgcgg caattgcaat gaacaagccg tggtcggaaa
    28801 aactgcacga tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag cagattgaag
    28861 aagaaaccgg cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg
    28921 aagcgaaagc ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt
    28981 ctatcgccaa gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggca
    29041 acatcggtct gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca
    29101 cctacgcaac ctggtggatc cgtcaggcga tcacccgctc tatcgcggat caggcgcgca
    29161 ccatccgtat tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc
    29221 agatgctgca agagatgggc cgtgaaccaa cgccggaaga actggctgaa cgtatgctga
    29281 tgccggaaga caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa
    29341 cgccgatcgg tgatgatgaa gattcgcatc tgggggattt catcgaggat accaccctcg
    29401 agctgccgct ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac gacgtgctgg
    29461 ctggcctgac cgcgcgtgaa gcgaaagttc tgcgtatgcg tttcggtatc gatatgaaca
    29521 ccgaccacac gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa cgtatccgtc
    29581 agatcgaagc gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa gtgctgcgta
    29641 gcttcctgga cgattaatcg gtaggccgga tcaggtgtta cgccgcaccc ggcactagtc
    29701 cctccgcaca aacgccacct tttcggtggc gcgaaaattg atgtaatgca atgaaaaata
    29761 aaggattatt tttctcgttg tccacatttt gccatagcga tacatcttag cccgtaatga
    29821 ttcgcgggct aagcatatac aaaatgttaa gcctgatgtt ggaaaaagtt tgtttttcct
    29881 ttgtgtagga aagaacaaag ggtggcggaa agttcagaaa gccagcgatt atggcgtaat
    29941 tattctgaca gaagaacaat ttatcaattt tattgatact ggtgaaattc ctcttgagca
    30001 agactgcgtg gttgagaatg atatcagtaa atcgcttgag aaggatgtta acacttacaa
    30061 gagtatgata gatgagttct cgctttctat ccgtactgta agggagtttc gccgtagtaa
    30121 tgcgttgatt gcggtatttg ttgatggtta cgcagctggt tggcgctttg ctgtaaaaga
    30181 gtgttacaag gattctttgg atataaaact taccccagta aaaacagagt tttttaatta
    30241 tgaaatttgg acgcaagggg attcctttca gtttaatcgc ggtgatgttt tttatagtga
    30301 tcgcctagcg tatgattctt attgcgattt tttaaagctt gatgatgctg tcgttcttca
    30361 ggtgaaatat gagtgctttt ctggttacga ttctgtaagt acttttgatg gtgtgatgga
    30421 tggtgagttt tctttttctc gtatggaacc cagacctgtc cataaattac ctgtgctgat
    30481 tgaaactcaa ctgtatgacc ccggaaagat tattgttgat gtttttgttc ctgatgctgc
    30541 gaaaaaacaa ttaaaactta aagacacctt agagctgaag caggatgaat ttgtctgttt
    30601 acttcagtc
//
LOCUS       DAZGTJ010000058        30498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000058 DAZGTJ010000000
VERSION     DAZGTJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.058"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            258..800
                     /gene="ecpR"
                     /locus_tag="ACHK8E_003165"
                     /gene_synonym="matA"
     CDS             258..800
                     /gene="ecpR"
                     /locus_tag="ACHK8E_003165"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP0345396.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            874..1461
                     /gene="ecpA"
                     /locus_tag="ACHK8E_003166"
                     /gene_synonym="matB"
     CDS             874..1461
                     /gene="ecpA"
                     /locus_tag="ACHK8E_003166"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP0345397.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1518..2186
                     /gene="ecpB"
                     /locus_tag="ACHK8E_003167"
                     /gene_synonym="yagY"
     CDS             1518..2186
                     /gene="ecpB"
                     /locus_tag="ACHK8E_003167"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP0345398.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2212..4737
                     /gene="ecpC"
                     /locus_tag="ACHK8E_003168"
                     /gene_synonym="yagX"
     CDS             2212..4737
                     /gene="ecpC"
                     /locus_tag="ACHK8E_003168"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP0345399.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4727..6370
                     /gene="ecpD"
                     /locus_tag="ACHK8E_003169"
     CDS             4727..6370
                     /gene="ecpD"
                     /locus_tag="ACHK8E_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP0345400.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6339..7049
                     /gene="ecpE"
                     /locus_tag="ACHK8E_003170"
     CDS             6339..7049
                     /gene="ecpE"
                     /locus_tag="ACHK8E_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP0345401.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7362..7691
                     /gene="ykgJ"
                     /locus_tag="ACHK8E_003171"
     CDS             7362..7691
                     /gene="ykgJ"
                     /locus_tag="ACHK8E_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0345402.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7703..7869)
                     /locus_tag="ACHK8E_003172"
                     /pseudo
     CDS             complement(7703..7869)
                     /locus_tag="ACHK8E_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7940..8554)
                     /gene="yagU"
                     /locus_tag="ACHK8E_003173"
     CDS             complement(7940..8554)
                     /gene="yagU"
                     /locus_tag="ACHK8E_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0345403.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8769..8954)
                     /locus_tag="ACHK8E_003174"
     CDS             complement(8769..8954)
                     /locus_tag="ACHK8E_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345404.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            9003..9160
                     /locus_tag="ACHK8E_003175"
                     /pseudo
     CDS             9003..9160
                     /locus_tag="ACHK8E_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9175..9250)
                     /locus_tag="ACHK8E_003176"
     tRNA            complement(9175..9250)
                     /locus_tag="ACHK8E_003176"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9215..9217),aa:Thr,seq:cgt)
     gene            complement(9365..10618)
                     /gene="proA"
                     /locus_tag="ACHK8E_003177"
     CDS             complement(9365..10618)
                     /gene="proA"
                     /locus_tag="ACHK8E_003177"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP0345405.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(10630..11733)
                     /gene="proB"
                     /locus_tag="ACHK8E_003178"
     CDS             complement(10630..11733)
                     /gene="proB"
                     /locus_tag="ACHK8E_003178"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP0345406.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12022..13077
                     /gene="phoE"
                     /locus_tag="ACHK8E_003179"
     CDS             12022..13077
                     /gene="phoE"
                     /locus_tag="ACHK8E_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0345407.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(13116..13517)
                     /gene="crl"
                     /locus_tag="ACHK8E_003180"
     CDS             complement(13116..13517)
                     /gene="crl"
                     /locus_tag="ACHK8E_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0345408.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(13575..14819)
                     /gene="frsA"
                     /locus_tag="ACHK8E_003181"
     CDS             complement(13575..14819)
                     /gene="frsA"
                     /locus_tag="ACHK8E_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0345409.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(14911..15369)
                     /gene="gpt"
                     /locus_tag="ACHK8E_003182"
     CDS             complement(14911..15369)
                     /gene="gpt"
                     /locus_tag="ACHK8E_003182"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0345410.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            15630..17087
                     /gene="pepD"
                     /locus_tag="ACHK8E_003183"
     CDS             15630..17087
                     /gene="pepD"
                     /locus_tag="ACHK8E_003183"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0345411.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(17144..17496)
                     /gene="prfH"
                     /locus_tag="ACHK8E_003184"
                     /pseudo
     CDS             complement(17144..17496)
                     /gene="prfH"
                     /locus_tag="ACHK8E_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(17492..17698)
                     /locus_tag="ACHK8E_003185"
                     /pseudo
     CDS             complement(17492..17698)
                     /locus_tag="ACHK8E_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(18016..18468)
                     /gene="yafP"
                     /locus_tag="ACHK8E_003186"
     CDS             complement(18016..18468)
                     /gene="yafP"
                     /locus_tag="ACHK8E_003186"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0345412.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(18465..19520)
                     /gene="dinB"
                     /locus_tag="ACHK8E_003187"
     CDS             complement(18465..19520)
                     /gene="dinB"
                     /locus_tag="ACHK8E_003187"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0345413.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(19591..20376)
                     /gene="lafU"
                     /locus_tag="ACHK8E_003188"
     CDS             complement(19591..20376)
                     /gene="lafU"
                     /locus_tag="ACHK8E_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP0345414.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            20348..22060
                     /locus_tag="ACHK8E_003189"
                     /pseudo
     CDS             20348..22060
                     /locus_tag="ACHK8E_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(22278..22775)
                     /gene="rayT"
                     /locus_tag="ACHK8E_003190"
     CDS             complement(22278..22775)
                     /gene="rayT"
                     /locus_tag="ACHK8E_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFP0345415.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(22952..23701)
                     /gene="yafL"
                     /locus_tag="ACHK8E_003191"
     CDS             complement(22952..23701)
                     /gene="yafL"
                     /locus_tag="ACHK8E_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0345416.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            24013..24753
                     /gene="dpaA"
                     /locus_tag="ACHK8E_003192"
     CDS             24013..24753
                     /gene="dpaA"
                     /locus_tag="ACHK8E_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP0345417.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(24724..25491)
                     /gene="yafJ"
                     /locus_tag="ACHK8E_003193"
     CDS             complement(24724..25491)
                     /gene="yafJ"
                     /locus_tag="ACHK8E_003193"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP0345418.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(25607..26185)
                     /gene="lpcA"
                     /locus_tag="ACHK8E_003194"
     CDS             complement(25607..26185)
                     /gene="lpcA"
                     /locus_tag="ACHK8E_003194"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP0345419.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            26425..28869
                     /gene="fadE"
                     /locus_tag="ACHK8E_003195"
     CDS             26425..28869
                     /gene="fadE"
                     /locus_tag="ACHK8E_003195"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP0345420.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTCDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(28912..29358)
                     /gene="ivy"
                     /locus_tag="ACHK8E_003196"
     CDS             complement(28912..29358)
                     /gene="ivy"
                     /locus_tag="ACHK8E_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP0345421.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            29539..30309
                     /gene="yafV"
                     /locus_tag="ACHK8E_003197"
     CDS             29539..30309
                     /gene="yafV"
                     /locus_tag="ACHK8E_003197"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP0345422.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
     gene            complement(30379..>30498)
                     /locus_tag="ACHK8E_003198"
     CDS             complement(30379..>30498)
                     /locus_tag="ACHK8E_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0345423.1"
                     /translation="AINILTNDNVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         7596 a         7862 c         7770 g         7270 t
ORIGIN      
        1 cattttttgt aaatattgac ttaaccatgg aattcatttt ctgttcacat attgacactc
       61 atcaggaaaa aaacataaat ttaaacctaa tcgaaataat taaaacttaa tctcgtttaa
      121 cctatattga tatgtgctac gtatcttatt tacttccgat ttactaaaga aactgaatgt
      181 acctgtaaaa attacaggtt tggaaagtag tgacatggca aaatgattac agcagggact
      241 atgaggttaa aaaccatatg gaatgtcaaa accgttctga taaatacatc tggtctcccc
      301 atgacgccta cttctataaa ggactatctg aactgattgt ggatatcgac agattaattt
      361 atctatcgct ggagaaaatc agaaaagatt tcgtgtttat caatctcaat acggattctt
      421 taactgagtt tataaaccgt gataatgagt ggttatccgc ggtaaagggg aaacaggtcg
      481 tattgattgc ggccagaaag tcagaagcct tagcaaatta ttggtattac aacagcaata
      541 ttaggggcgt ggtatacgct ggactgagtc gtgatattag aaaagaactg gcctatgtga
      601 ttaatggcag gttcctgaga aaagatatta agaaagataa aatcactgac cgggaaatgg
      661 aaattatccg catgacggct cagggaatgc tgcctaaatc gattgccaga attgaaaatt
      721 gtagtgtgaa gacagtgtat acccatcggc ggaatgcaga ggccaagctg tactcaaaat
      781 tatataagtt ggttcagtaa actccaagca agttagtttt aaaaatgact caccgggact
      841 tcatgtcctc aattcaactc gggaagaaaa gcaatgaaaa aaaaggttct ggcaatagct
      901 ctggtaacgg tgtttaccgg tacaggtgta gcgcaggctg ctgacgtaac agctcaggct
      961 gtagcgacct ggtcagcaac agccaaaaaa gacaccacca gtaagctggt tgtgacgcca
     1021 ctcggtagcc tggcgttcca gtatgccgaa ggcattaaag gttttaactc acaaaaaggt
     1081 ctatttgacg tggctatcga gggtgactca acggctaccg cctttaaact gacctcacgc
     1141 cttatcacca acaccttaac ccagttggat acctcaggtt ccacactgaa tgtgggcgtg
     1201 gattataacg gcgcggcagt cgaaaaaact ggcgataccg tgatgatcga taccgccaac
     1261 ggcgtactgg gcggcaacct tagcccactg gctaacggtt acaatgccag caatcgtacc
     1321 accgcacagg atggtttcac tttctccatc atcagcggca ccaccaatgg taccaccgca
     1381 gtaaccgatt acagcactct accggaaggc atctggagcg gcgacgttag cgtacagttc
     1441 gacgctacct ggaccagtta atctctctga tgtaccagca ggggtacccc cctgctttat
     1501 tcctggatgg aattgttatg aaaaagcacc ttctgcctct cgctctgctg ttttccggaa
     1561 tatcaccggc ccaggcgctg gatgtcggcg atatatcatc gtttatgaac agtgacagca
     1621 gcacgctaag caaaacgatc caaaacagta ccgacagtgg ccgcctcatc aatatccgtc
     1681 tcgaacggct ctcttcaccg cttgacgacg ggcaggttat cgcaatggac aagccggatg
     1741 agttgctact cactcccgcc agcttgctgc tacccgccca agccagcgaa gtgatccgct
     1801 tcttctataa gggaccagca gatgaaaaag agcgctacta ccgcattgtc tggtttgatc
     1861 aggccctcag tgatgcgcag cgcgataatg ccaaccgcag cgctgtggcc actgcttccg
     1921 cccgcatcgg caccattctg gtcgtcgcac cccgccaggc aaactaccac tttcagtacg
     1981 ccaacggctc cctgacaaat acaggaaatg cgacgctgcg gatcctcgcc tacggcccct
     2041 gcctgaaagc cgccaatggt aaggagtgta aagagaatta ctacctgatg ccgggcaagt
     2101 cgcgtcgttt tacccgcgtg gacacggcgg ataacaaagg acgggttgca ctttggcagg
     2161 gtgataagtt cattcccgtg aaatagatag ctgtgcagat ggataacgac aatgccttta
     2221 cgacggttct ccccaggact gaaagcccag tttgccttcg gcatggtctt tttgttcgtt
     2281 cagcccgatg ccagcgctgc tgacataagt gcgcagcaaa taggtggggt gattattccg
     2341 caggccttca gtcaggcgct ccaggacggc atgagcgtcc cgctttatat tcatctcgcc
     2401 ggtagccagg gtcgccagga cgatcagcga atcggcagcg cttttatctg gttggatgat
     2461 ggacagttac gcatccgaaa aatacagctg gaagagagtg aagataacgc cagtgtcagc
     2521 gaacaaactc gacagcagct gatgactctg gccaacgccc cgttcaatga ggcccttacc
     2581 atccccctga ctgacaacgc gcagttggat ctcagcttgc gccaactgct gctgcagctg
     2641 gtggtcaagc gcgaagcgct gggcaccgta ctacgctcac gtagcgaaga catcgggcag
     2701 tccagtgtta acaccctcag cagtaatctg agctataact tcggcgtcta taacaaccag
     2761 ttgcgtaacg gcgggagcaa cacctccagc tatctgtcgc tgaataacgt tactgcgctg
     2821 cgcgaacatc acgtggtgct cgacggctca ctgtacggga ttggtagcgg tcaacaggac
     2881 agtgaattat ataaagcgat gtatgaacgc gattttgctg gtcaccgatt tgccggtgga
     2941 atgctcgaca cctggaactt gcagtcctta gggccgatga ccgccatttc agcagggaag
     3001 atttacggcc tttcctgggg aaaccaggcc agctccacca tcttcgacag cagccagtcg
     3061 gccacgccag tgatcgcctt tttaccggcg gcgggcgaag tacatctcac ccgtgatggg
     3121 cggctactaa gcgttcagaa cttcaccatg ggcaatcatg aagtggatac ccggggtcta
     3181 ccgtacggta tttacgatgt ggaagttgag gtaatcgtta acggtcgcgt gatcagcaaa
     3241 cgcacccagc gggtcaataa gctgtttagc cggggacgtg gcgtcggtgc accgctggcg
     3301 tggcagatat ggggcggtag ctttcatatg gatcgctggt cggaaaacgg gaaaaagacg
     3361 cgaccagcca aagagagttg gctggcaggt gcctcgacct ccggctcact gagtacgttt
     3421 agctgggcgg caacgggata tggatacgat aatcaggcgg tgggtgaaac ccgtctgacg
     3481 ctgccgcttg ggggggcgat caacgttaac ctgcaaaaca tgctggccag tgacagctca
     3541 tggagcaaca tcgccagcat cagcgccact ctacctggag gcttcagttc gctgtgggtt
     3601 aaccaggaaa aaacccgcat tggcaatcaa ttgcgacgta gcgatgccga caaccgtgca
     3661 atcggcggca cactcaacct gaactcactg tggtcgaagc tgggtacgtt cagcatcagc
     3721 tacaatgatg accgccgcta caacagccat tattacacgg cagattacta tcaaagtgtc
     3781 tacagcggta cctttggttc gcttggcctg cgggccggta ttcagcgcta taacaacggc
     3841 gacagcagcg ccaatacagg gaaatatatc gctctcgatc tctcgctacc actgggcaac
     3901 tggtttagcg cagggatgac ccatcaaaac ggctacacca tggcaaacct gtcagcacgc
     3961 aaacagtttg atgaaggaac cattcgcact gttggtgcca atctgtcacg agccatctcc
     4021 ggcgataccg gtgatgacaa aaccctcagc ggtggggcgt atgcacagtt cgacgctcgt
     4081 tacgccagcg gaacgctgaa cgtcaatagc gcggcggacg gctacatcaa tactaatttg
     4141 accgccaacg gcagcgtcgg ctggcagggt aaaaacatcg ctgccagcgg gcggaccgat
     4201 ggcaacgctg gggtgatatt cgacactggc ctggagaacg atggtcagat cagcgccaaa
     4261 atcaacgggc ggattttccc gcttaacggc aagcgtaact atctcccgct ctctccctat
     4321 ggaagatatg aggtggagtt gcagaacagc aaaaactcac tcgacagtta cgatatcgtc
     4381 agcggtcgca aaagtcatct gactctctat ccaggcaatg tcgctgtcat tgagccagag
     4441 gtgaagcaga tggttaccgt ctccggtcgt atccgtgcgg aagacggcac actgctggct
     4501 aacgcacgga ttaacaacca tatcggccga acccgaaccg atgaaaacgg cgagtttgtc
     4561 atggacgtgg ataagaaata ccccactatc gattttcgct acagtggcaa taaaacctgc
     4621 gaagtggcac tggaactcaa ccaggcgcgc ggtgccgtct gggtcggtga tgtggtctgc
     4681 agcggcctct catcgtgggc ggcggtgacg cagacaggag aagagaatga gagttaacct
     4741 actgatagcg atgataatct ttgcgctaat ctggcctgca accgcgctca gagcggcagt
     4801 gagcaaaaca acctgggcgg atgctccggc acgcgagttt gtgtttgtcg aaaacaactc
     4861 agacgacaac tttttcgtca ctcctggcgg ggcgctggat ccgcgcctga ccggtgccaa
     4921 ccgctggacc ggtttaaaat acaatggttc aggaaccatc tatcagcaaa gcctcggcta
     4981 cattgataac ggttacaaca ccggccttta taccaactgg aagtttgata tgtggctgga
     5041 aaattcacca gtttcatctc ctttaactgg cttgcgctgc atcaactggt acgcagggtg
     5101 taatatgacc accagtctta tcctgccgca aaccaccgac gccagtggat tttatggcgc
     5161 gacggtgacc agcggcggcg cgaaatggat gcacggcatg ttgtcagacg cgttttacca
     5221 gtatctgcaa caaatgcccg tcggcagcag ctttacaatg accatcaatg cctgccagac
     5281 ctctgtgaac tatgacgcca gcagcggcgc acgctgtaag gatcaggcct ccggcaactg
     5341 gtatgttcgc aacgtcaccc atacgaaagc agcaaatctg cggttgataa atacccactc
     5401 gctggcggaa gtatttatca acagcgacgg agtaccgact ctgggcgaag ggaacgccga
     5461 ctgccggacg caaaccatcg gcagccgttc aggattaagt tgtaagatgg ttaactatac
     5521 cctgcaaaca aacggactca gcaacacctc aatccatata ttcccggcga tcgccaactc
     5581 gtcgttagcc tcggccgtcg gggcgtacga tatgcagttc agtctgaatg gcagttcatg
     5641 gaaaccggtg agcaatactg cctattacta caccttcaac gagatgaaga gcgcagactc
     5701 gatctatgtt ttcttctcga gcaacttctt taaacagatg gtgaacctcg ggatcagcga
     5761 tatcaacacc aaagatctat tcaactttcg ctttcagaac accacatcac cggagtctgg
     5821 ctggtatgaa ttttctacct ccaacacgct gattatcaaa ccccgtgatt tcagcatcag
     5881 tattatctcc gatgaatata ctcagacacc gtcgcgggag ggatatgttg gcagcggcga
     5941 gtcggcactc gatttcggct atatcgtaac caccagcggt aaaacagctg ccgacgaagt
     6001 gctgatcaag gtgaccggac ccgcgcaggt gattggcggg cgctcctatt gtgtcttcag
     6061 ctccgatgac ggtaaggcga aagtaccgtt cccggcgacg ctttccttta ttacccgcaa
     6121 cggagctaca aaaacctacg atgccgggtg cgatgatagc tggcgggata tgaccgatgc
     6181 gctgtggttg accacaccgt ggactgatat ctctggcgaa gtggggcaga tggataagac
     6241 cacagtcaaa ttttcgattc caatggataa cgccatttct ctgcgtacgg tagatgataa
     6301 cggctggttt ggcgaagtca gcgcttcagg agagattcat gttcaggcga cgtggcgtaa
     6361 cattaactaa ggccctgctg acagtggtct gtatgctggc ggcacctttg acacaggcga
     6421 tttcggtcgg caatctgact ttttcactgc cgtccgagac tgactttgtc agcaaacgtg
     6481 tcgtgaataa caacaaaagc gcgcggatat accgtattgc catcagtgct attgatagcc
     6541 cgggcagcag tgaattgcgc acccgaccgg tggatggtga actacttttc gccccccgcc
     6601 agctggcgtt gcagactggt gagagcgagt attttaaatt ttactatcat ggtccacggg
     6661 ataaccgcga gcgctactac cgggtctcat ttcgcgaggt ccccactcgt aacctgacaa
     6721 aacgcagccc aaccggcggt gaggtcagca cggagccggt ggtggtgatg gataccattc
     6781 tggtagtacg accacgtcag gttcagttta aatggtcctt cgatcaggtg accggaacgg
     6841 taagtaacac cggcaacacg tggtttaagc tactgattaa accaggatgt gattcgaccg
     6901 aagaggaagg cgatgcctgg tatctacgtc cgggagacgt ggttcatcag cctgagttac
     6961 gtcagccggg gaatcattat ctggtctata acgacaaatt cattaagatt agcgattcct
     7021 gtccggctaa gcccccttcg gcggactaag acttatccat cggacgaggg aagaattcca
     7081 cctcagagac tcaaaaatcg tttaaatgat gtggaagcga tcgtcaatgt cacctgtaac
     7141 gatgagcttt ctcatatgca caccacagtg ttcaggagac acggaaaggt accaggcggc
     7201 ctgctaacga gtggtttctt cactaattag gacgtaaaat cggtaacggc tggaaatcat
     7261 tcaatactca cactatcgaa agtacaccag ccaaccgcga tacatccctg catacgacgt
     7321 accgcagttc tctttacgat ttatagctgt actggtgaat tatgagcaat ctgaatccat
     7381 gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc tttttactgg gccgaagctg
     7441 acgatgctgg cggtaccatt cccgccaggc tcactgaaca aatatcccct tttcaccgat
     7501 gcatgagcgg tactaatcag aaaaatcccc gatgtattgc ccttgcagga acaccgggca
     7561 aaaatgcctg ctgcacgata tatgaaaatc gatcgtccac atgcagagaa ttcgccatgt
     7621 ctggtgagaa cggagaagtc aatgaggctt gcaatcgtgc aagagctaaa tacgggctaa
     7681 caccgttata aatatacaat aattaattgc attgccccgc cagctgtaat agcggggctt
     7741 cgtttttcag ggacaacaaa acctgaaact tcttccattt tccagcaaca atgcctcttc
     7801 ttctgtagcg ttctccggat ctacatcatc ctgaagtagc gcgatatgat cactgactgt
     7861 ccgcatcaga atatgaaaag caacgttttt aacctataaa aatggcgcta tatttgcgcc
     7921 atttttatca ttcaaagcat tatctatttg agcctaaagg gatctcagga tctggctcat
     7981 gggtaattct gtttcgtaaa tctctgcgaa taatttcaat agaccagaac cagactaaat
     8041 gtccaaaaat ttcagagaca ttctcatacc acggaagatc aaagagaggt ggcgtaagtc
     8101 ccatgagagg gaatgaaatc atatgaacaa aaagttgcgc taaagcacct gccagtaagc
     8161 cctgccaaag tttaattttc ggaaatactt cggcgaccac acagtaacca acggcaaaaa
     8221 ctatcgaaaa gattatgtgc gttacaccaa cccagttaaa gacatgcccg gcgaaggtat
     8281 aaacagccga atttggatct gtcagtccca accaatcccg aagaaaaatg tacggaggat
     8341 tgagaaagtt acgggagcaa tcaatttggc ctgcagccct gattaatgat tccggaccac
     8401 acgctgcatt aaacatatca accggactac gtggtggcaa tggaacttct gccccccact
     8461 taacaaatgc ggaaacaacg ccagcaatca gcccaatgaa tgcagcaaga ccataacgtc
     8521 tgcggtttgg tggagtttgt tcaaatatat tcatatctac cctgcttgta ccattatgtt
     8581 atacacctct ttaggagtat tcataaaaca aggcaaatgt aaagaactgt attgttttgt
     8641 ataaccacat ggttacctaa tcgccaatta atataagctc cattatttct ccatattttc
     8701 atgttagaag tgacaaggag aaatgtaatg tttctctcag agaatataaa tcattccttt
     8761 tgcccacctt aatggtgggc ttttttatgc aaaacctgaa cgtcctgtgg tattcctctg
     8821 gcataatgat tttcgcgatt aaccaacaac cagaccagca aagacgccac cacaagcaca
     8881 gtaacatcca tcaggcttgc cacaccccat gcgtgcggct caaagccgcc atggcatatt
     8941 aatccgcttg ccattttcga acagctgatt ggtttggtcc ggtgtttccc tgcttgcact
     9001 taaaaatgag cggatgggaa actctcgaaa tggttcgtcg atatgctcac ctcgcaccta
     9061 atcaccttac cgaacacgca cggtaaatag attcgatcct gaacccatcg gtcccaaatc
     9121 tgtcccaata aaaaataagg aaggtattag tgatgtgtaa cttattgatt taaatggtgc
     9181 cgataagagg agtcgaacct ccgaccttcg cattacgaat gcgctgctct accaactgag
     9241 ctatatcggc cctgaaagga catgttcacg aacgtgaatc acggtggaca aggttaaaac
     9301 taaccgggcg atgcgtcaat ggccttgtga atcaaatggc tacttttgca tcacccggtt
     9361 ttatttacgc acgaatggtg taatcaccaa tgccgatcca cttgtaagtg gtcagtgctt
     9421 ccagccccat tgggccacgc gagtggagtt tttgtgtgct taccgccact tccgcaccca
     9481 gtccaaactg gccgccgtcg gtaaaacgcg tagaggcgtt aacgtaaaca gcggaggaat
     9541 ctacttcgtt aacaaaacgc tgggcgttgc gcatatcgcg ggtcaggatc gcatcggagt
     9601 gttgcgtgcc gtgttcacga atatgggcga tggcatcgtc aagatcgctg acgattttga
     9661 cgttcaaatc taatgacaga aactcatcgt catactcttc ggttttaaca gcaaccacct
     9721 tcgcggggcc tgtctgcaac tgcgccagtg cacctgcatc agcgtgtaac gtcacgccac
     9781 tttccgccat ttgtttgctt aatgcgggca ggaaactatc ggcgatgttt ttattcacca
     9841 gcaacgtttc aaccgtatta catgtgctcg gtcgctgagt tttcgcgttg acgatcactt
     9901 tcagggcttc agcgatctct gcactttcat caacgtaaat atggcatacg cctataccac
     9961 ctgtgatcac cgggatcgtc gactgttcgc ggcacagttt atgcaggcca gcgccaccgc
    10021 gcgggatcag catgtcgatg tatttatcca tacgcagcat ttcactgacc agcgcgcggt
    10081 caggattatc aatcgcctgc acggcacccg ccggtaagct gcaggatttc agggcgtcct
    10141 gaatcaccgc caccgttgca gcgttagtgc gacacgtttc tttgccgccg cgcaggatca
    10201 ccgcgttacc tgttttcagg cacagcgaag cgacatctac cgtcacgttc gggcgcgctt
    10261 cataaatcac gccaatcacc cccagcggca cgcgacggcg ctccagacgc agaccactgt
    10321 ccagtacgcc gccatcgatt acctgcccca ccggatcggc gagattacac acctgacgca
    10381 catcgtcggc gatgcctttc agccgtgcgg gcgtcagtgc cagacggtca agcatcgctt
    10441 cgctaaggcc attggcacgc gcgtcagcaa catcctgggc gttagcgttg aggatgattt
    10501 cgctttgtgc ttccagttca tcggcgattt tttccagcac acgatttttt tcgcggctgg
    10561 agagttgcgc taatttatac gaggcttgct ttgcggcaat gcccatttgt tccagcatcg
    10621 gccagctcct taacgggtaa tcatgtcatc acggtgaacg gcaaccgggc catattcata
    10681 tcccagtatt gcatcaattt cttgcgagtg gtgtccggca atgcggcgta atgcatcgct
    10741 gttgtaacga ctgacgccgt gggcgatatc gcgaccttcg aggttgcaaa tgcggatgac
    10801 ttcaccacgc gagaaattgc cagttacgct cttaatgcct ttcggcaaca gggagctgcc
    10861 gcgttcaaga atggcggcgg ttgccccttc atctaccgtg atttcacccg ccggcggcgc
    10921 accgaaaatc cagcgtttac ggttttcaag cggagtcgcc tgggcatgga acagcgtacc
    10981 gacggaaatg ccttccatca catcaccaat aacgcccggc ttgctgcccg cggcaataat
    11041 ggtgtcgata cccgcacggc aagccacgtc cgcggcctgc aatttggtgc tcataccgcc
    11101 agttccgagg cctgaaacgc tatcaccggc aatcgcgcgc agtgcgtcat caatgccgta
    11161 cacatcttta atcagttctg cctgcggatt gctgcgcggg tcagcggtat acaaaccttt
    11221 ttgatcggtc agcagcaaca gtttatcggc acccgccaga atcgccgcca gcgcagaaag
    11281 gttatcgtta tcaccgacct taatctctgc cgtagcgaca gcatcgttct cattaattac
    11341 cggaacgata ttgttatcga gcaacgcacg cagggtatcg cgggcgttca ggaagcgttc
    11401 acgatcttcc atatcagcac gggtcagcaa catctgcccg acatgaatgc cgtaaattga
    11461 aaacagctgt tcccacagtt gaatcagtcg actctgccct accgccgcca gcagctgttt
    11521 tgaagcgata gtcgctggca attccgggta ccccaggtgc tcacgtccgg cggcaatcgc
    11581 acccgacgtc acaataacaa tccgatgccc ggcggcatgt aactgcgcgc actggcgaac
    11641 aagttcaacg atatgggcac ggttcaggcg gcgcgatccg cctgttagca cactggtgcc
    11701 gagttttacc accagcgtct ggctgtcact catgattctc tgccattcaa ttttaggaaa
    11761 aaatgatatc aaacgaacgt tttagcagga ctgtcgtcgg ttgccaacca tctgcgagca
    11821 aagcatggcg ttttgttgcg cgggatcagc aagcgtagcg gcagttgttt acgcttttat
    11881 tacagattta ataaattacc acattttaag aatattatta atctgtaata tatctttaac
    11941 aatctcaggt taaaaacttt cctgttttca acgggactct cccgctgaat attcgcgcgt
    12001 taattaaaat caggaatgaa aatgaaaaag agcactctgg cattagtggt gatgggcatt
    12061 gtggcatctg tatccgtaca ggccgcagaa atatataaca aagacggtaa taaactggat
    12121 gtctatggca aagttaaagc catgcattat atgagtgata atgacagtaa agatggcgac
    12181 cagagttata tccgttttgg ttttaaaggc gaaacacaaa ttaacgatca attgaccggt
    12241 tatggtcgct gggaagcaga gtttgccggt aataaagcag agagtgatac tgcacagcaa
    12301 aaaacacgtc tcgcttttgc cgggttgaaa tataaagatt tgggttcttt cgattatggt
    12361 cgtaacctgg gcgcgttgta tgacgtggaa gcctggaccg atatgttccc ggaatttggt
    12421 ggcgactcct cggcgcagac cgacaacttt atgaccaaac gcgccagcgg tctggcgacg
    12481 tatcggaaca ccgacttctt cggcgttatc gatggcctga acttaaccct gcaatatcaa
    12541 gggaaaaacg aaaaccgcga cgttaaaaag caaaacggcg atggcttcgg cacgtcattg
    12601 acatatgact ttggtggcag cgatttcgcc atcagtggag cctataccaa ctctgatcgc
    12661 accaacgagc agaacctgca aagccgtggc acaggcaagc gtgcagaagc atgggcaaca
    12721 ggtctgaaat acgatgccaa taatatttat ctggcaactt tctattctga aacacgcaaa
    12781 atgacgccaa taactggcgg ctttgccaat aagacgcaga actttgaagc ggtcgctcaa
    12841 taccagtttg actttggtct gcgtccatcg ctgggttatg tcttatcgaa agggaaagat
    12901 attgagggca tcggtgatga agatctggtc aattatgtcg atgttggtgc tacgtattat
    12961 ttcaacaaaa atatgtcagc gtttgttgat tataaaatca accaactgga aagcgataac
    13021 aaattaaata ttaataatga tgatattgtc gcggttggga tgacgtatca gttttaatga
    13081 atattgccgg atgtgatgca tccggcacat ttcattcacg ccgttaactt caccggttca
    13141 tcacgaaaat catctgccgg ttccagcttc agattcagcg tcgccagcag ctcacgcagc
    13201 ttctcgtgaa attcacgcag ggtgtgctcc agtcgttcaa ccacttcggc atcttttatc
    13261 tgaacactcg tccagtcgcc tgctttatcg aacagaccaa actggtaact gtaggtaaaa
    13321 cgggattcct gcgcttccag ttccatccac cagccccaga actcacgcac ttccggtgcc
    13381 ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga atcgattatc ttcgcactta
    13441 ccttcacgaa tatacgggcc tagtgcggta aattttttga tcaatctgct cttcgggtgt
    13501 ccactcggta acgtcattgc aatctcctgt tgtgatgcaa ctgttttacc aaattggtaa
    13561 aatttagcaa atttttaaca caaacgtttt tcgatccagt ctgtgatttc ctgaaggcct
    13621 ttgtcaaaat tccgatacac cgggttaaat gggatctcta ataatttacc gtcagcagat
    13681 gacgacgtga ttaagcgtga gtcctcttcc gggctgaacg gatcgttctt ccagtagcct
    13741 gataacattg gcgttgggca gcgacgtccc agcaatcctt gcacttttaa tgaatagcga
    13801 ttcaactcca ctcgcaacgc ttcatcggaa gcatcatgca tccctaaacg actggccaga
    13861 acgtcaagat acatttccgg cacctgttgc tggcacttaa aatcactcaa cagggtatga
    13921 actaccggac caagacaggc aaccactttc agacgcggcg attcaaggta tgccagacgc
    13981 acggcaacgt tagcgccgaa acggaaacca aaggccgcga cgcgagtgtg atccacccac
    14041 ggtacgttag gcagcgcctt taaaacgtgc tgatgcaaca ggctggagtc ctgggtgagc
    14101 ttccattttg aagaaaagcc caccgacggc atatcaatag tcagcatagc aatgccgcgc
    14161 ggcgcaaaat aacgttcata caggctgtaa tagtccgtct gcatcgcatc cagaccacca
    14221 cacattaata ctgtcgggaa cgggccatcg cctttcggca tatgcaaaaa gccggtgatg
    14281 ggcgcaccgc cgggtacggt aaactccatc tgccgcatcg tgcccggcag acgctgagcg
    14341 gcctcttcat aggcgcggtt tgacaaagcc tgcgcttgct cggccaggtc atctcctttc
    14401 agatgaggat aggcagcaat gttgtacaac gtagccgcat gcagccagta tcgaccgctg
    14461 agttgcggat cttcttccgc acaggctttt tgttgccaca ccatcgcctg ggtagcccac
    14521 tcataaatcc agttgccgcc gcggtagcca atcaccgtat cgtataaatc atcgtcggta
    14581 cgttcggcat cgctcatcac aatacgcgcc tggacgtcga ggatttcacg ggggtcaatg
    14641 ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat ggttttacca
    14701 tcaagggccg actgcacagg cagttgtgcg ccgtgactaa agcggcggac tagcgtcgag
    14761 gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg
    14821 gatgcagcct ccagaatact tactggaaac tattgtaacc cgcctgaagt taaaaagaac
    14881 aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg gcacgaatac
    14941 gacgcccata tcccacggct gttcgatcca ggtattttgc gggatatcaa caacataatc
    15001 atcaaccagc ggacgaccag ccggttttgc gaagatggtg acaaagtgcg cttttggata
    15061 catttcacga atcgcaaccg cagtaccacc ggtatctacc aggtcatcaa taacgatgaa
    15121 gccttcgcca tcgccttctg cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc
    15181 gtagctggaa atacaaacgg tatcgacatg acgaataccc agttcacgcg ccagtaacgc
    15241 acccggtacc agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat
    15301 cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt
    15361 ttcgctcatg tgaagtgtcc cagcctgttt atctacggct taaaaagtgt tcgaggggaa
    15421 aataggttgc gcgagattat agagatctgg cgcactaaaa accagtattt cacatgagtc
    15481 cgcgtctttt tacgcactgc ctctccctga cgcgggataa agtggtattc tcaaacatat
    15541 ctcgcaagcc tgtcttgtgt tgacaacatt ttctgctaac cctgtgacct gcaatactgt
    15601 tttgcgggtg atcgacaagg agacttaacg tgtctgaact gtctcaatta tctccacagc
    15661 cgctgtggga tatttttgcc aaaatctgtt ctattcctca cccgtcctat catgaagagc
    15721 aactcgctga atacattgtt ggttgggcaa aagagaaagg tttccatgtc gaacgcgatc
    15781 aggtaggtaa tatcctgatt cgtaaacctg ctaccgcagg tatggaaaat cgtaaaccgg
    15841 tcgtcttgca ggcccacctc gatatggtgc cgcaaaaaaa taacgacacc gtgcatgact
    15901 tcaccaaaga tcctatccag ccttatattg atggcgaatg ggttaaagca cgcggcacca
    15961 cgctgggtgc ggataacggc attggtatgg cctctgcact ggcggttctg gctgacgaaa
    16021 acgtggttca cggcccgctg gaagtgctgc tgaccatgac cgaagaagcc ggtatggacg
    16081 gtgcttttgg cttacagagc aactggttgc aggctgatat tctgattaac accgactccg
    16141 aagaagaagg tgaaatctac atgggttgtg cgggaggtat cgacttcacc tccaacctgc
    16201 atttagatcg tgaagcggtt ccagctggct ttgaaacctt caagttaacc ttaaaaggcc
    16261 tgaaaggcgg tcactccggc ggtgaaatcc acgttggcct gggtaatgcc aacaaactgc
    16321 tggtgcgctt cctggcgggt catgcagaag aactggacct gcgccttatc gatttcaacg
    16381 gcggcacact gcgtaacgcc atcccgcgtg aagcctttgc gaccattgct gtcgcagctg
    16441 ataaagtcga cgctttgaaa tctctggtga atacctatca ggagatcctg aaaaacgagc
    16501 tggcagagaa agagaaaaat ctggccttgt tgctggactc tgttgcgaac gataaagctg
    16561 ccctgattgc gaaatctcgc gataccttta ttcgtctgct gaacgccacc ccgaacggtg
    16621 tgatccgcaa ctccgacgtg gcaaaaggtg tggtcgaaac ctccctgaac gtcggtgtgg
    16681 tgaccatgac tgacaataac gtagaaattc actgcctgat ccgttctctg atcgacagcg
    16741 gtaaagacta cgtggtgagc atgctggatt cgctgggtaa actggctggc gcgaaaaccg
    16801 aagcgaaagg cgcatatcct ggctggcagc cggacgctaa ttctccggtg atgcatctgg
    16861 tacgtgaaac ctatcagcgc ctgttcaaca agacgccgaa catccagatt atccacgcgg
    16921 gcctggaatg tggtctgttc aaaaaaccgt atccggaaat ggacatggtt tctatcgggc
    16981 caaccatcac cggtccacac tctccggatg agcaagttca catcgaaagc gtaggtcatt
    17041 actggacact actgactgaa ctgctgaaag aaattccggc gaagtaatta tttgatttgc
    17101 tgccggatgg cgtttaatcg ccttccggca gtttcatcct tcattatcct tcgataaaag
    17161 ccatccctgt gaacgttcgt cgcgggttgc cacgttcaat ctggtgatgg aacattcgcc
    17221 gctgcgattt cagcgccgcg ctattttcct gttgctgttg ttccagcttc caggcaatca
    17281 gcaatcgcgc cagccgcttg ttagcatgcc cagaaaccag tttttgcgcc catgatgagg
    17341 ccgatacgga ctcggacaaa tacactgaat agtgccgcac caactttcgc ttaatgccca
    17401 tgcattatcg ccatccagag aaatcagcgc cgaacgcagc gtgtcagagt agcggcccgt
    17461 ttctgtttcc agcaccgtta ccgcgacgtc ttgccgggta aaaacgacca acagagaaga
    17521 acgctgcgcc aatcgggtag ccgcgtccgg gatgtaaatc tggcatcccc acaacgcgct
    17581 gcatgttggg taaatttgcc gtggtgtgta attgttggat cgctaaactc tcgatccaca
    17641 ggtcatcaga tgcgatggta aataccgcat cggataaggg acgaatatat ttgcccatgt
    17701 atcattccat ttatactcgt catacttcaa gttgcatgta ctgcgaccgc gttcgttcac
    17761 cccagtcact tactttagta agctcctggg gattcactcg cttgtcgcct tcctgcaact
    17821 cgaattattt agagtatata aatataaaaa tcaggaaatg gcagacgaat ccagggcaag
    17881 aaaaaaagaa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga tttgtcatga
    17941 tttacctcct tgcagtttgt ggaataagaa aacgcgcaca taccaggcgg gtgttatttt
    18001 cattgcaagc tggatttaat gttgcggttt atatcgcata gaaaaattag taaaccacgc
    18061 tcccctgcat tcaacgtgct gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg
    18121 ttttgcggtt atgctggcgt ccaccgtaag ttcggattca gacttaatca aaggttttaa
    18181 caaggcgctg gcaaccccgc ggcgggtgta ttcagggtca acaaataaca tatcgatata
    18241 gtgttcaacg caggtaataa agccgaccag ttttgcatta atgactgcaa cccgcacttg
    18301 tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg
    18361 ttgtggtgaa taatgctgac tggcggtcat cgtaaccgct ctaaggaaaa tagcgcatag
    18421 ttgctgaaaa tcgccaggct gatagtctct tatttgtatg ttattcataa tcccaacacc
    18481 agttgtcttt ccatttgcgg gtcaagtaac gtcacatgca gccccaccag tcgcacaccg
    18541 cgcccgccgc ggcgttcatc ccaggtttta cgcgcagtgg cgattagatc agctttattc
    18601 agccgtggcc agacgtgctc ctgagtagtt tgctgaaaat cgtcgaactt taatttcact
    18661 ccctgacggg cgatcagtaa atcaggtttc acctttgcca gacggcgttc aagttcagga
    18721 tacagccgct cgataatcgc ttcacattca gaccagtgat gaatatcttc cgccatcgtg
    18781 cgttccacgc cgacggattt tcgcaaccgt tcactgttaa cgtcgcgctc gtcaatcccc
    18841 tgactacgct cccacaaaat gcggccaaat ttgccaaagc gtttaagcag aatcaccaga
    18901 tcacactttt gcacatcacc gcaggtccgc agccccatcg cttccagttt tgccgccgag
    18961 actttgccga cgccgggaat ttttgccagc ggtaaggttt gcagaaatgc cggaacttct
    19021 gccggcgtaa tcacaaactg accgttgggt ttattcatgt cggaggcgat tttggcgaga
    19081 aactttactg gtgccacgcc cgcagatgcc gtcagttgca gctcgttgaa gatcgtctgg
    19141 cggatttcct gggcgatgag ggtcgcagaa ccgtggcaat ggacgctatc agtgacatcg
    19201 agataggctt catccagtga caacggttca atacgcgagg tgtagcgcga gaagatttca
    19261 cggatatgat ttgaggcttc tttgtaggcg tcaaagcgcc ccggaagcaa ggtgagatgc
    19321 gggcataatt tgagcgccat ccctgtcggc atagcgctac gtacgccaaa tttacgcgcg
    19381 ggataattgg cggtgctgat taccccccgt cgttcgcggc tgccgccaat agcaataggg
    19441 atatcgcgca gggcgggatt gtcgcgcatc tccaccgctg cgaaaaagca gtccatatcc
    19501 acatgaatga ttttacgcat tgctcacctc tcaacactgg taaagtatac agtgatttca
    19561 gggtttgaga aatgcgtaaa gattcagcat ttaacgcgct cgctgcgaaa gcacctgctg
    19621 cttatccagc ttttgcacca gcggctgcac cactttatcc ccatgctgac caaagtattg
    19681 atacagcgta tctgacgcac ttttggtcag caccataatc tcaatgcgcc ggttgcccgc
    19741 gctttgcgga tttttggcat ccagcagcat ctggtccgcc attgcgctta cctgcatcac
    19801 tttattttcc ggcattccgg cctcttccag cacccgacga gccgaaagcg cgcgatcgcc
    19861 cgaaaggttc cagttgttgt agatattgtt tttgtaggcc atcgcatcgg tatgcccggt
    19921 aataataatt ttgttatcga gcgagtcgaa cactggcgcg agctccacca gcagcgtttt
    19981 aaagaacggc ataatcttcg cgctgccgcg ttcaaacata ttgcggttct ggtcatcttt
    20041 aatcagcacg cgcagtccct ggggaacaat ctccatctcc agattcgctt ccatatgtgc
    20101 atcacgggca atggtgttga tactggtcgc cagctcgcca agctcggtgg cggatttctt
    20161 cttcagcagc gccgttttct cgttgacgtc gcgcgctttt ttctccgtct cttccggcac
    20221 cgccacggtg gcaggttttg gcggatgaga cgggctgatt ttatttaacg gcgacaaccc
    20281 accaccgtta aaaatcgact gcccgtgcag cgcggcgata atgctttcac gctcggattt
    20341 actgacgcta ttgacaatca actttatcgt cgtcaccaaa ggggccgagc gtatttccga
    20401 ggtttccgcc cgctttacct tagacgcgat gcccggtaag cagatggcga ttgacgccga
    20461 tcttaacgcc gggttgatca accaggcgca ggcgcaaacc cggcgtaaag atgttgccag
    20521 cgaggccgat ttctacggcg caatggacgg ggcatcgaag tttgtgcgcg gggacgccat
    20581 cgccgggatg atgattctgg cgatcaacct gatcggcggc gtctgtatcg ggatcttcaa
    20641 atacaacctg agcgccgacg ccgccttcca gcaatatgtg ctgatgacca tcggcgacgg
    20701 gctggtggcg cagatccctt ccctgctgct ctccaccgcg gcggcgatta tcgtcacccg
    20761 cgtcagcgac aacggcgata tcgcccatga cgtgcgccac cagctgctgg caagcccgtc
    20821 ggtgctctac accgccaccg gaattatgtt tgtactggcg gtggtgccgg gaatgccgca
    20881 cctgccgttt ttgctgttca gcgccctgct tggctttacc ggctggcgga tgagcaaacg
    20941 cccgcaggcg gcggaggcgg aggagaaaag tcttgaaacg ctaacccgca ccatcactga
    21001 aaccagcgag caacaggtca gttgggaaac cattccgctg atcgagccta tcagcttaag
    21061 cctcggctac aagctggtgg cgctggtcga taaagcgcag gggaacccac tcacccagcg
    21121 gattcgcggc gtgcggcagg tgatatccga cggcaacggc gtgctgctgc cggagatccg
    21181 tattcgggaa aacttccgcc tcaagcccag ccagtacgcc atattcatca acggcattaa
    21241 ggccgatgaa gcggatattc cggcagataa actgatggcc ctgccctcca gcgaaaccta
    21301 cggcgagatt gacggcgtac tggggaatga cccggcatat gggatgccgg tcacctggat
    21361 tcagcctgcg cagaaagcga aggcgctgaa tatggggtat caggtgatcg acagcgccag
    21421 cgtgattgcc acccatgtga ataagattgt gcgcagctat attcctgatt tatttaacta
    21481 tgacgatatt acgcagttgc ataaccgcct gtcgtcgatg gcaccgcgac tggcggaaga
    21541 cttaagcgca gcgctcaatt acagccagtt gctgaaagtg tatcgcgcgc tgctgaccga
    21601 aggcgtttcc ctgcgcgata ttgtcaccat cgccaccgtg ctggtcgcca gtagtgcggt
    21661 gactaaagat catatcctgc tggcggcaga tgtgcgcctg gcgctgcggc gcagtatcac
    21721 ccatccgttc gttcgcaagc aggagctgac ggtgtatacg ctgaataatg agctggaaaa
    21781 tctgctgacc aacgtggtga atcaggcgca acaggccggc aaagtgatgc tcgacagcgt
    21841 gccggtggac ccgaatatgc ttaaccagtt ccagagtacg atgccacagg tgaaggagca
    21901 gatgaaagcg gcggggaaag acccggtgct gctggtgccg ccgcaactgc gtccattgct
    21961 ggcgcgctat gcaaggttgt ttgcgccggg gctgcatgtg ctgtcgtata acgaagtgcc
    22021 ggatgagctg gagttgaaga ttatgggggc gttgatgtaa gaatccggca tcggtgcaca
    22081 attgccggat gcggcgtgaa cgccttatcc ggcctaccgt taaggcatag atttgtaggc
    22141 atgataagac gcgctagcgt cgcatcaggc atctgcgcac aaccgccgga tgcggcgtga
    22201 acgccttatc cggcctaccg ttaaggcaca gatttgtagg ccggataagc gtaagcgcat
    22261 ccggcaccgg cgcacaatta taaaataata cgctccccgg cactaaaatc cgttacgtcc
    22321 cccgcccaat cgatgggata taaacctttc gcgacatcgc gatggaacgt tgagaatggc
    22381 caatcactca cctgctttac ccagccatgt ttcactggat ttatgtaaat ataatcaaca
    22441 tgatgacgat aatctttggt attgcgaatg gcatgctccc aaaaacgcgg ttgccacata
    22501 ttttgcaatc cacaagcatg ggtgaactgt tttttaatct cccgccagcg agaggaaaaa
    22561 tcatcatcgc tttcgggtaa tgtccagata cagtgcatat gttccggcaa aacgacccag
    22621 gcgttaattt caaaaggtct ggcctgctta actttaataa tggcgttacg gagtgtctgg
    22681 tactgggtgg tcaaaagttg gcttcgacga ttccgtaaat ttaccgtgaa aaaccatgtt
    22741 ccccccttga tgtaataacg acgatattca gacatggcga ctcctttttc tcaacgataa
    22801 gaagaaaagg aaaagtcgac aatgaagttg tgttatatcg ggaaagcagc tcgcaaaaaa
    22861 ttcgcagact cgtaggcatg ataagacgcg gcagcgtcgc atcaggcatc ggtgcactat
    22921 tgccggatgc ggcgtgaacg ccttatccgt cctacaaaat cgtttcctcc gtcaaaatcc
    22981 tgcgcgcgcc taaaaaatgg tcctgccaga aaggttcggc taaccgactt acccgaatgg
    23041 tttccccggt gcgcggtgac tcgataaact gaccgtctcc aagatatacg cccatatggt
    23101 cggctatctc acgactgtgg atatggaaaa acagcaaatc tccccggcgc aggtcgttat
    23161 tcgccacaat cgttgcccga tggtagtgat acatctcatt ggccgtacgc gggagcttcg
    23221 cctcgaggat cttgttgtag gcataaaaaa ccaacccgct acagtcgaag ccttgatcgg
    23281 gccgcgtgcc gccccagacg taaggcttac caagctgctg ctccaggcgg tgaatcgcca
    23341 cttcggtgat gttgtgtaga tggtcgctgc ttaagaaatg gttgttctcc gccagcgcct
    23401 gccagcgtct gtcactcgcc ttgagcggcg caggaaacca ttctggatgc tgttttatct
    23461 gctcccgatt atgctggcgc agcgcccttc tgcttttggc attgccttcc acaatatagc
    23521 tggcctgctt tttcagatgg gtttggtact gttttaataa acgcgcacgg ttttgcatcc
    23581 gctggcgggc ggcgtagctg aaagagatat gtgatgtggt ggcgctggcg agtaaaggga
    23641 gcaaaacgca taacgaaaac acgcctggat gcagaaatct gccgtgcaag aacgaagaca
    23701 tgaaggacat aaaccagctg aatgaaggtg agaattttaa aagaccggag gagtaaacgg
    23761 gattattaat tgcttatttg catattacta ttgcggtttc ataatcttag gaaataagaa
    23821 ataaatctac aatccatgcg cttttcggac tcttgaaaac tgcccctgtg ctaaatttgt
    23881 aaaccctgcg ccgacaggct tgcgtaaaac tcttttggtg ataatgtgag ccttccaaat
    23941 ccagactatt ccgatttggg attcattttg ccgtcctggc gtgtgtaacc gttttatcaa
    24001 ggaataagca gtatgcgtaa aatcgcatta attcttgcga tgttgttgat cccgtgcgtt
    24061 tcgtttgccg gtttgctggg cagcagtagc tcgaccacgc cagtgagcaa agagtataag
    24121 cagcagttga tgggatcccc tgtctacatc cagatcttca aggaagaacg tacgctcgat
    24181 ctctacgtca aaatgggcga gcaatatcaa ctgctcgaca gctataaaat ctgtaaatat
    24241 tccggcggct tagggccaaa acagcgtcag ggcgatttca aaagcccgga agggttttat
    24301 agcgtccagc gtaatcagtt aaaaccagac agccgttact acaaagcgat taatattgga
    24361 ttcccaaacg cctatgaccg tgcgcatggt tatgaaggga aatacctgat gattcacggc
    24421 gattgtgttt ccatcggctg ctacgcgatg accaatcagg gtattgatga gatcttccag
    24481 ttcgttactg gtgcgctggt gtttggtcag ccgagcgtgc aagtgagtat ttacccgttc
    24541 cgtatgaccg acgccaatat gaagcgccat aaatattcca actttaagga cttctgggag
    24601 caactgaagc caggctacga ctactttgag caaacccgta agccaccgac cgtttctgtg
    24661 gtcaatggcc gttacgtggt cagcaagccg ttaagccacg aagttgtgca gccacaactg
    24721 gcatcaaact acacgctccc cgaggcaaaa taagcgccat tcacctggca taatcttttg
    24781 ccaggtttca ttgcccgtca gcggctgtgt cgcaatcacc gtgaccacat cattcggtgt
    24841 ggtctgcgag ctgaagtcga tttccacatc ctgatccagc aacgttgcca cgccaaacgg
    24901 cgcgcggcgg gtgatccagt gtaaattagt cgagcaatac gccattacat agcgcccgtc
    24961 agaaagcagc atgttgaaaa cgcccttctg ccgcagttca tccgccagtg aggcgatata
    25021 tttaaacact gccgccatgt tgcccggtgt gcgcgggtaa cgctgcgtta atttatgcag
    25081 gagccagcaa aaggcttttt cgctatcggt ttcaccgacc gggcggaagt tgccggtttc
    25141 cagtgatttg tagcccgtca gttgtccatt atgggcataa gtccagttac gcccccataa
    25201 ctcgcgggta aacgggtgag tattttccag cgccacctcg ccccgattag cctggcgaat
    25261 atgagccacc accgaacagg attttatcgg gtagtcctgg acaagtttgg cgatggggga
    25321 attaaagctg ggttgcggat ctttaaatgt gcgacagcct ttaccttcgt aaaaggtaat
    25381 gccccagcca tctttatgtg gcccggttcc accaccacgc tgtacaagtc cggtgaaact
    25441 aaagcagata tcggtaggga cgttggcgct catcccgagc aattcgcaca tgggtaacct
    25501 ccacaacagc gggagcgcat ggcccccggc gaagattgat taaaaagtac aaaggttcag
    25561 gctgttaccc tcaccccggc cctctccctg aaagggagaa aagaccttac ttaaccatct
    25621 ctttttcaat taactggatc aggatatgga tcactttaat gtggatctcc tgaatgcggt
    25681 cggcataacc aaagtgcggt acgcgaattt cgatatccgc tgtgccagcc attttgccgc
    25741 cgtctttacc ggtcagggtg atcactttca tccccttctc acgcgccgcc gcgatcgctt
    25801 tgatcacgtt tgcagagtta ccggaggtgg agatccccag cagtacatcg ccttcgcgac
    25861 ctaccgcttc aacgtaacgg gagaaaatat cattgaaacc aaaatcgtta ccgacgcagg
    25921 aaatatggct gacgtcagaa atggcaatcg ccgggtagcc cggacggttt tcacggtagc
    25981 gaccggtcaa ctcttcggca aagtgcatag cgtcgcaatg ggagccgccg ttgccgcagg
    26041 aaagcacttt gccaccggct ttaaagctgt ctgctaacag gaccgccgcg cgctgaatgg
    26101 cgtgaatatt ggcgtcatct tttaaaaaat tagccagcgt ttccgccgct tcgttcagtt
    26161 cgttacgaat aagatcctgg tacatgagga tatccttcag cataaatgta atagacaaaa
    26221 tgcagtgtac cggataccgc caaaagcgag aagtacgggc aggtgctatg accaggactt
    26281 tttgacctga agtgcggata aaaacagcaa caatgtgagc tttgttgtaa ttatattgta
    26341 aacgtattgc taaatgtttt tacatccact acaaccatat catcacaagt ggtcagacct
    26401 cctacaagta aggggctttt cgttatgatg attttgagta ttctcgctac ggttgtcctg
    26461 ctcggcgcgt tgttctatca ccgcgtgagc ttatttatca gcagtctgat tttgctcgcc
    26521 tggacagccg cactcggcgt tgctggtctg tggtcggcgt gggtactggt gcctctggcc
    26581 attatcctcg tgccatttaa ctttgcgcct atgcgtaagt cgatgatttc cgcgccggta
    26641 tttcgcggtt tccgtaaggt gatgccgccg atgtcgcgca ctgagaaaga agcgattgat
    26701 gcgggcacca cctggtggga aggcgatttg ttccagggca agccggactg gaaaaagctg
    26761 cataactatc cgcagccgcg cctgaccgca gaagagcaag cgtttctcga cggcccggta
    26821 gaagaagcct gcaggatggc gaatgatttc cagattaccc atgagctggc ggatttgccg
    26881 ccggagctgt gggcgtatct taaagagcat cgcttcttcg cgatgatcat caaaaaagag
    26941 tacggcgggc tggagttctc ggcttatgcc cagtctcgcg tgctgcaaaa actctccgga
    27001 gtgagcggga tcctggcgat taccgtcggc gtgccaaact cattaggccc gggtgagcta
    27061 ctgcaacatt acggcactga cgagcagaaa gatcactatc tgccgcgtct ggcgcgtggt
    27121 caggagatcc cttgctttgc actgaccagc cccgaagcgg gttccgatgc gggcgcgatt
    27181 ccggataccg ggattgtttg catgggcgag tggcagggcc agcaggtgct ggggatgcga
    27241 ctgacctgga acaaacgcta cattacgctc gcgccgattg cgaccgtgct tggtctggcg
    27301 tttaaactct ccgacccgga aaaactgctg ggtggcgcag aagatttagg tattacctgt
    27361 gcgctgatcc cgaccaccac gccgggcgtg gaaattggtc gtcgccactt cccgctgaac
    27421 gtgccgttcc agaacggacc gacgcgcggt aaagatgtct tcgtaccgat cgattacatt
    27481 atcggcggac ccaaaatggc cgggcaaggc tggcggatgc tggtggagtg cctctcggta
    27541 ggccgcggta tcaccctgcc ttccaactca accggcggcg tgaaatcggt agcgctggca
    27601 acgggtgcgt atgcgcacat ccgccgtcag ttcaaaatct ctattggtaa gatggaaggg
    27661 attgaagagc cgctggcgcg tattgccgga aacgcttatg taatggatgc tgcggcatcg
    27721 ctgattacct acggcattat gctcggtgaa aaacctgccg tgctgtcggc tatcgttaag
    27781 tatcactgta cccaccgtgg gcagcagtcg attattgatg cgatggatat cactggtggc
    27841 aaaggcatta tgctcgggca aagtaacttc ctggcccgtg cttaccaggg cgcaccgatt
    27901 gccatcaccg ttgaaggggc taacattctg acccgcagca tgatgatctt cggacaagga
    27961 gcgattcgtt gccatccgta cgtgctggaa gagatggaag cggcgaagaa caatgacgta
    28021 aatgcgttcg ataaactgct gttcaaacat atcggtcacg tcggtagcaa caaagttcgc
    28081 agcttctggc tgggcctgac gcgcggttta accagcagca cgccaacctg cgatgccact
    28141 aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct ttctgatgtc
    28201 tcgatggcgg tgctgggcgg cagcctgaaa cgtcgtgagc gtatctcggc acgtctgggg
    28261 gatattttaa gtcagcttta cctcgcctct gccgttttga agcgttatga cgacgaaggc
    28321 cgcaacgaag ccgacctgcc gctggtgcac tggggcgtac aggatgcgct gtatcaggca
    28381 gaacaggcga tggacgattt actgcaaaac ttcccgaacc gcgtggttgc cgggctgctg
    28441 aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa gctggatcat
    28501 aaggtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg tcgcggtcag
    28561 tacctgacgc cgagcgagca taacccggtt ggtttactgg aagaggcgct ggtggatgtg
    28621 attgccgccg acccgattca tcagcggatc tgtaaagagc tgggtaaaaa cctgccgttt
    28681 acccgtctgg atgaactggc gcacaacgcg ctggcgaagg ggctgattga taaagatgaa
    28741 gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt tgatgacttt
    28801 gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg gaaagttgaa
    28861 gccgcgtaat tgaataacgg agccgaaagg ctccgtttct ttatccgcta attatttaaa
    28921 attaaagcca tccggatggt tttccaggct gccggtcaac gccgcgaaca gcaccgtttt
    28981 accatcaata gaaagcgcat cgttcacatt caaccaggtg agtttctctt gcgacgtttt
    29041 ctcatcaata gtcgagaaca gccccgtcat ctgattagat ttctcggacc acatcacagc
    29101 gatacgttgc gagccacagt catgcggttt gcacgcgctc atcacctgat acgtctcatc
    29161 tcccaatgtc acggtttgtg cgggagtata agtaccgcct ttcatcaccc aggcaggcag
    29221 cttatgccct tgcaccatct gattaaacgc cgctttggtg gtttcgccct ttgcaagact
    29281 gctaatggtt aaatcatcct gcgccattgc actggtggcg atgaccagag cggcgactgt
    29341 cgttattgcc ttaaacatca ttcctcccgg gcttatcctg cccatgtgat taactcccta
    29401 aagcctggcg ataacacgat aatttgcaaa tcagatattt ccaatatcag catttgttat
    29461 caatttattc tgcgctaagg caactgcctg ataaggtgtt agagttataa aacccttcgc
    29521 ataaaggagt tgtacaccgt gcctggtttg aagattacgc ttttgcagca accactggtg
    29581 tggatggatg gtcctgccaa cctgcgccat tttgatcgtc aactggaagg tattaccggg
    29641 cgcgatgtga tcgttctacc ggagatgttt accagcggct ttgccatgga agcggcagct
    29701 tcgtcgctag cacaagatga cgtagtgaac tggatgaccg ctaaagcgca gcagtgcaat
    29761 gcgctgattg caggcagtgt tgcattacaa acggagtctg gttcggttaa ccgctttttg
    29821 ctggttgagc cgggcggcac ggtacatttt tatgataagc gtcatctgtt ccgcatggca
    29881 gatgagcatc tgcattataa agcgggcaat gagcgagtga ttgtggaatg gcgcggctgg
    29941 cgtattttgc cgctggtgtg ctacgactta cgttttccgg tgtggtcgcg caatctcaac
    30001 gattatgacc tcgccctgta cgtcgccaac tggcctgctc cgcgctctct gcactggcag
    30061 gcattgctga cagcccgcgc gattgagaat caagcgtatg tggcgggatg caatcgcgtc
    30121 ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc gggtgattaa tccgcaaggc
    30181 gagattatcg ctactgccga cgcgcatcag gcaacgcgca ttgatgcgga gctgtcgatg
    30241 atggcattgc gggaatatag agaaaagttt ccggcatggc gggatgcgga tgagtttagg
    30301 ttgtggtgag tgggggttgt gtttagggct ggggagagtc agggtaagac tacgaaagcc
    30361 cgctccccca gggaaagatt acgaaagccc gctcccagca aggactgacg ccaggtagtt
    30421 tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata cattatcatt
    30481 cgttaaaata ttaatagc
//
LOCUS       DAZGTJ010000059        30173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000059 DAZGTJ010000000
VERSION     DAZGTJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.059"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..248
                     /gene="tuf"
                     /locus_tag="ACHK8E_003199"
     CDS             <1..248
                     /gene="tuf"
                     /locus_tag="ACHK8E_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0345424.1"
                     /translation="KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNI
                     KMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            478..861
                     /gene="secE"
                     /locus_tag="ACHK8E_003200"
     CDS             478..861
                     /gene="secE"
                     /locus_tag="ACHK8E_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP0345425.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            863..1408
                     /gene="nusG"
                     /locus_tag="ACHK8E_003201"
     CDS             863..1408
                     /gene="nusG"
                     /locus_tag="ACHK8E_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP0345426.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1567..1995
                     /gene="rplK"
                     /locus_tag="ACHK8E_003202"
     CDS             1567..1995
                     /gene="rplK"
                     /locus_tag="ACHK8E_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP0345427.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1999..2703
                     /gene="rplA"
                     /locus_tag="ACHK8E_003203"
     CDS             1999..2703
                     /gene="rplA"
                     /locus_tag="ACHK8E_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP0345428.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3116..3613
                     /gene="rplJ"
                     /locus_tag="ACHK8E_003204"
     CDS             3116..3613
                     /gene="rplJ"
                     /locus_tag="ACHK8E_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP0345429.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3680..4045
                     /gene="rplL"
                     /locus_tag="ACHK8E_003205"
     CDS             3680..4045
                     /gene="rplL"
                     /locus_tag="ACHK8E_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP0345430.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4365..8393
                     /gene="rpoB"
                     /locus_tag="ACHK8E_003206"
     CDS             4365..8393
                     /gene="rpoB"
                     /locus_tag="ACHK8E_003206"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP0345431.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8470..12693
                     /gene="rpoC"
                     /locus_tag="ACHK8E_003207"
     CDS             8470..12693
                     /gene="rpoC"
                     /locus_tag="ACHK8E_003207"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP0345432.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12933..14066)
                     /gene="thiH"
                     /locus_tag="ACHK8E_003208"
     CDS             complement(12933..14066)
                     /gene="thiH"
                     /locus_tag="ACHK8E_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP0345433.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14063..14833)
                     /gene="thiG"
                     /locus_tag="ACHK8E_003209"
     CDS             complement(14063..14833)
                     /gene="thiG"
                     /locus_tag="ACHK8E_003209"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP0345434.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14835..15035)
                     /gene="thiS"
                     /locus_tag="ACHK8E_003210"
     CDS             complement(14835..15035)
                     /gene="thiS"
                     /locus_tag="ACHK8E_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP0345435.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15019..15774)
                     /gene="thiF"
                     /locus_tag="ACHK8E_003211"
     CDS             complement(15019..15774)
                     /gene="thiF"
                     /locus_tag="ACHK8E_003211"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP0345436.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(15767..16402)
                     /gene="thiE"
                     /locus_tag="ACHK8E_003212"
     CDS             complement(15767..16402)
                     /gene="thiE"
                     /locus_tag="ACHK8E_003212"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP0345437.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16402..18297)
                     /gene="thiC"
                     /locus_tag="ACHK8E_003213"
     CDS             complement(16402..18297)
                     /gene="thiC"
                     /locus_tag="ACHK8E_003213"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP0345438.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18378..18478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18530..19006)
                     /gene="rsd"
                     /locus_tag="ACHK8E_003214"
     CDS             complement(18530..19006)
                     /gene="rsd"
                     /locus_tag="ACHK8E_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP0345439.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19101..19874
                     /gene="nudC"
                     /locus_tag="ACHK8E_003215"
     CDS             19101..19874
                     /gene="nudC"
                     /locus_tag="ACHK8E_003215"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP0345440.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19914..20978
                     /gene="hemE"
                     /locus_tag="ACHK8E_003216"
     CDS             19914..20978
                     /gene="hemE"
                     /locus_tag="ACHK8E_003216"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP0345441.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            20988..21659
                     /gene="nfi"
                     /locus_tag="ACHK8E_003217"
     CDS             20988..21659
                     /gene="nfi"
                     /locus_tag="ACHK8E_003217"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP0345442.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21702..22292
                     /gene="yjaG"
                     /locus_tag="ACHK8E_003218"
     CDS             21702..22292
                     /gene="yjaG"
                     /locus_tag="ACHK8E_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP0345443.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22479..22751
                     /gene="hupA"
                     /locus_tag="ACHK8E_003219"
     CDS             22479..22751
                     /gene="hupA"
                     /locus_tag="ACHK8E_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP0345444.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22764..23459
                     /gene="yjaH"
                     /locus_tag="ACHK8E_003220"
     CDS             22764..23459
                     /gene="yjaH"
                     /locus_tag="ACHK8E_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP0345445.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23461..23880)
                     /gene="zraP"
                     /locus_tag="ACHK8E_003221"
     CDS             complement(23461..23880)
                     /gene="zraP"
                     /locus_tag="ACHK8E_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP0345446.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24118..25494
                     /gene="zraS"
                     /locus_tag="ACHK8E_003222"
     CDS             24118..25494
                     /gene="zraS"
                     /locus_tag="ACHK8E_003222"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP0345447.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25491..26816
                     /gene="zraR"
                     /locus_tag="ACHK8E_003223"
     CDS             25491..26816
                     /gene="zraR"
                     /locus_tag="ACHK8E_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP0345448.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26813..28102)
                     /gene="purD"
                     /locus_tag="ACHK8E_003224"
     CDS             complement(26813..28102)
                     /gene="purD"
                     /locus_tag="ACHK8E_003224"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP0345449.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28114..29703)
                     /gene="purH"
                     /locus_tag="ACHK8E_003225"
     CDS             complement(28114..29703)
                     /gene="purH"
                     /locus_tag="ACHK8E_003225"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP0345450.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7061 a         7832 c         8324 g         6956 t
ORIGIN      
        1 ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg cagttctact
       61 tccgtactac tgacgtgact ggtaccatcg aactgccgga aggtgtagag atggtaatgc
      121 cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg gacgacggtc
      181 tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc
      241 tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca tttgatgccc
      301 tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt cataatcatt
      361 gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg
      421 tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaatac ttctgacagg ttggtttatg
      481 agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt
      541 gtggtggcat tgctcctggt agcgattgtc ggcaactacc tttatcgcga cattatgctg
      601 ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt cgcgctgtta
      661 acgacaaaag gtaaagcaac cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag
      721 gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc tgcggttacc
      781 gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct ggtatccttt
      841 atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt acgtcgttca
      901 ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca
      961 caacatggaa gagttgtttg gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg
     1021 tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc tcgttcagat
     1081 ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat
     1141 cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa
     1201 ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ttgtttgaac cgggtgaaat
     1261 ggtccgtgtt aatgatggtc cgttcgctga cttcaacggc gttgttgaag aagttgatta
     1321 cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc ctgtagagct
     1381 ggacttcagc caggttgaaa aagcttaacc cagcgatcaa aaaagcggcg atttaatcgt
     1441 tgcacaaggc gcgagattgg aatacaattt cgcgcctttt gtttttatgg gccttgcccg
     1501 taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca acttgaggaa
     1561 tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc tggtatggct
     1621 aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat catggaattc
     1681 tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat tccggtagta
     1741 atcactgttt acgctgaccg ttctttcact ttcgttacca agactccgcc ggcagcagtt
     1801 ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa agacaaagtg
     1861 ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact
     1921 ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc catgggcctg
     1981 gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat ccgagagaaa
     2041 gttgatgcaa ccaaacagta cgacatcaac gaagctatcg ctctgctgaa agaactggcg
     2101 actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa
     2161 tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg ttccgttcgc
     2221 gtagccgtat ttacccaggg tgcaaacgct gaagctgcta aagctgcagg tgctgaactg
     2281 gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt
     2341 attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc
     2401 ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga agcggttaaa
     2461 aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat ccacaccacc
     2521 atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt
     2581 gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa agttagcatc
     2641 tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac
     2701 taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta taatgcttaa
     2761 tgcagacgta tatccgagat attcgggttg tggcaaggcg gtaactgagt gagtcgccag
     2821 gagcatagtt aactatgtga ctggtgcgaa cgaaggaagc caacgccgcc acaagctgaa
     2881 tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag
     2941 accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat
     3001 tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg
     3061 aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc
     3121 tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc
     3181 gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg
     3241 taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg
     3301 tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat
     3361 tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc
     3421 gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca
     3481 gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac
     3541 catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa
     3601 agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg
     3661 atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc
     3721 tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc
     3781 cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga
     3841 attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg
     3901 tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg caccggctgc
     3961 tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc
     4021 tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc
     4081 tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt
     4141 cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac
     4201 tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca
     4261 ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc
     4321 tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata
     4381 ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt
     4441 atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc
     4501 agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt
     4561 ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc
     4621 aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg
     4681 agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg
     4741 aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg
     4801 tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact
     4861 cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggcttgact
     4921 tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg
     4981 cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg
     5041 aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc
     5101 tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac gtagaaaaag
     5161 gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg
     5221 aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta
     5281 ccggcgagct gatctgtgct gcgaacatgg agctgagcct ggatctgctg gctaagctga
     5341 gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat cacggcccat
     5401 atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa
     5461 tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa agcctgttcg
     5521 agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt atgaagttca
     5581 accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa gacgacatca
     5641 ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg
     5701 accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg
     5761 ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata
     5821 ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct
     5881 tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc
     5941 acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg
     6001 aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatagaa acccctgaag
     6061 gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct
     6121 tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa attcactacc
     6181 tatctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag
     6241 aaggccactt cgtagaagac ctggtaacct gccgtagcaa aggcgaatcc agcttgttca
     6301 gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt
     6361 ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc
     6421 aacgtcaggc cgttccgact ctgcgcgctg acaagccgct ggttggtact ggtatggaac
     6481 gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt
     6541 acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag
     6601 caggtatcga tatctacaac ctgaccaaat acacccgttc taaccagaac acctgtatca
     6661 accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg
     6721 gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc
     6781 cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag
     6841 accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg
     6901 ggccagaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg
     6961 atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt ctggttggta
     7021 aggttacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct
     7081 tcggtgagaa agcgtctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg
     7141 gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc
     7201 tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga
     7261 tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag
     7321 ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg accgacgaag
     7381 agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg
     7441 agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg
     7501 tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga
     7561 tggcaggtcg tcatggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc
     7621 cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc
     7681 gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg
     7741 acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc
     7801 agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg
     7861 atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg
     7921 tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga
     7981 cttctggtca gatccgcctg tacgacggcc gcactggtga acagttcgag cgtccggtaa
     8041 ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc
     8101 gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg
     8161 gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca
     8221 ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtact aagatgtata
     8281 aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat
     8341 tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc
     8401 tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg
     8461 agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt
     8521 gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa
     8581 gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc
     8641 tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc
     8701 ctgaaacacc gtggtgtcat ctgtgagaag tgcggcgttg aagtgaccca gactaaagta
     8761 cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg
     8821 aaatcgctgc cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga tatcgaacgc
     8881 gtactgtact tcgaatccta tgtggttatt gaaggcggta tgaccaacct ggaacgccag
     8941 cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac
     9001 gcgaaaatgg gtgcggaagc aatccaggcc ctgctgaaga gcatggatct ggagcaagag
     9061 tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg
     9121 accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg
     9181 atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt
     9241 ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac
     9301 cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt
     9361 atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc
     9421 ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt
     9481 ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgta
     9541 ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc
     9601 aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg
     9661 aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc
     9721 gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt
     9781 gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat
     9841 aacgccgact tcgatggtga ccagatggca gttcacgtac cgctgacgct ggaagcccag
     9901 ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa
     9961 ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt
    10021 gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat
    10081 cgctctggtc tggcctctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa
    10141 gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc
    10201 attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt
    10261 aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc
    10321 gttatttttg cggaccagat catgtacacc ggctttgcat atgcagcgcg ttctggtgca
    10381 tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca
    10441 gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa
    10501 cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg
    10561 atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt
    10621 tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt
    10681 cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg
    10741 ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac
    10801 ggtgctcgta aaggtctggc ggataccgca ctgaaaacgg cgaactccgg ttacctgact
    10861 cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtactcat
    10921 gaaggtatca tgatgactcc ggttatcgaa ggtggtgacg ttaaagagcc gctgcgcgat
    10981 cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc
    11041 gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc
    11101 gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac
    11161 tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt
    11221 atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc
    11281 ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc
    11341 atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc
    11401 cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagta
    11461 ccttacggtg cggtactggc aaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt
    11521 gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt
    11581 actgacatga tcgacggtca gaccattacg cgtcagaccg acgaattgac cggtctgtct
    11641 tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca
    11701 ctgaaaatcg ttgatgctca gggtaacgat gtactgatcc cgggtaccga tatgcctgcg
    11761 cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct
    11821 ggtgacaccc tggcgcgtat tccgcaggaa tctggcggta ccaaggacat caccggtggt
    11881 ctgccgcgcg ttgcggatct gttcgaagca cgtcgtccga aagagccggc aatcctggct
    11941 gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc
    12001 actccggtag acggtagcga tccgtacgaa gagatgattc caaaatggcg tcagctcaat
    12061 gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgatggtcc ggaagcgccg
    12121 cacgacattc tgcgtctgcg tggtgttcat gctgttactc gttacatcgt taacgaagta
    12181 caagacgtat accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt
    12241 cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggt
    12301 gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa
    12361 gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag
    12421 tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt
    12481 gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc
    12541 ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa
    12601 gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg
    12661 aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
    12721 tcgcttcggc gggttttttt atgggtgtta ttcagagggc aattcttcac ggaattttta
    12781 agaaaggcag ggcgaaacga ggaagaagct tttcgccctg gacaacattc ctgctgacgg
    12841 gactaccata aaaaaacaca acgctttcaa caaccttgcg tttttcatct tccgtagcgg
    12901 ctcggaataa atttacgttg ttacattctg tctcatggcc tttgcgaggc gcgtcccaga
    12961 tagctgtccc agtctttcca taccggctgc aaaccctgag cagttaacgc ggcagcaacc
    13021 gcttccggtc tgcgatcgtc gtgcggtgag aactgttcca gctcggggtg attgtcggca
    13081 tagccacctg gctgcgtttt cgaaaaagcg ctgacgttat taattgccag cggaataacg
    13141 cgatcgcgaa accacggtga ttcccgcgtg gagagtgaca gttcaatctc cggtgcaagc
    13201 agccggaagg cgcagatggt ttgcactaac tggcgttcat ccataatcga cgcaggctca
    13261 atgccgccag tacatggacg caggcgtggg aaggagatag agtagcggct ttgccagtaa
    13321 tgctgttgca gccatagcaa atgttctgca accatatagc agtcaacgcg ccagctgtcg
    13381 gaaaggccaa ttagcgcgcc gaggcctatc ttatcaatcc ccgcacggcc cagccgatcc
    13441 ggcgtttcca gccgccagaa gaagtcctgt tttttacctt tcagatgatg gcgggcataa
    13501 gtcgcctcgt gatatgtctc ctgataaacc atcacgccat ccagacctag ttgctttaac
    13561 tcggcgtatt ccgtctccgc cagcggttgc acttccatct gtagtgaaga gaactgttca
    13621 cgcagggcag ggagatgacg acgaaagtaa tccatcccca ctttcacctg atgttcacca
    13681 gtgactaata gcagatgttc aaagcccatc tcccgtatag cggcgctttc cctggcaata
    13741 tccgcttcat ccagcgtttt gcgcttgatg cgattgctca tggaaaatcc gcagtacgtg
    13801 cagtcgttag cgcaaagatt ggaaagataa agcgggacgt agaagctgac gacgttgcca
    13861 aatcgctgac gggtcagacg ctgcgcccgt tgggccagtg gttccagata gccactggcg
    13921 gctggcgata acagcgccat catatcgtcg cgggtgagtt gcgaggcatt tagcgcccgc
    13981 tctacgtcag cagccgtttt gctgttgata cgcaggcgga tgtcgtccca gtccagttgt
    14041 cgccagcgat cgctgaaggt tttcatgctg atgcctccag aaatccggtc agcgggctgg
    14101 tggcatgagc aaaatgactg cggctgcccg gtccggactg acgtgccagc aggcctgctt
    14161 ctactgccag acgaaatgcc ttcgccatgt tgacgggatc gtccgcgacg gcaatcgccg
    14221 tattcactaa caccgcgtcg gcccccattt ccagcgcctg cgcggcatgg ctgggaacgc
    14281 cgatgccagc atcgacaacc accggaaccg tggcttgctg ggtaataatc tccagcatgg
    14341 cacgggtttc cagtccctga ttcgagccaa tcggcgcgcc gagcggcatc accgctgcac
    14401 agccgacttc ttccagacgt ttacacaata ccggatcggc cccgcagtaa ggcagcacga
    14461 caaatccctg ttgtaccagc gtttcggcgg ctttcagggt ttcgatggga tcgggcaaca
    14521 gccagcgggc gtcagggtga atctctaatt ttaaccagtt tgtgcctaac gcttcacgag
    14581 ccagatgggc ggcgaaaatg gcttcttccg ctgttttcgc cccggatgta tttggcagca
    14641 gggtcacacc cgccgcgata agcggttcga ggatagcgtc gttgtgctgg cgcaagttga
    14701 cgcgtttcat cgccagtgtc accagttggc tgccgcaagc gcggatcgcc tccatcatca
    14761 gttgtgaaga ggcgaatttc cctgtgccgg taaacagatg tgaatcaaac gttttgtccg
    14821 caatacgtaa catctcaacc ccctgcaata acctgaaaaa gcaggatctg gtcgccatcc
    14881 tgcacgatat gttgcgccca ctgctcacgc gggacgattt gctgattaat cgccagagcc
    14941 gcgcccgatt gtcgttggtc gagttgctcc agtagttcgt gaaccgtttg cccggcggcg
    15001 cactgcatcg gttgatcgtt aaacaggatc tgcattgctt cctccgcata ccgggcaacc
    15061 actggcgcgg cgcaacgcca ggttgcgcca ctggctcgat ttaccgtcga acagtcggag
    15121 ttctcccgca ggcgtctcta taccgcttaa taacttaatg gcttccagtg cctgtaaagt
    15181 gcccataacc ccgaccaccg ggccaaccac gcccgccgtg cggcagttgc gttcaggctc
    15241 ctggttatct ggccacaggc agcggtaaca cccctgctcc cagggcggcg tcagtaccat
    15301 caactgaccg ccaaatccga ccgcgctggc ggtgataagc ggcgtgttga gtgccacgca
    15361 ggcggcatta atctcctgac gagtcgccat attgtcggta cagtcgagca ccacatcggc
    15421 ccgtgctacc gcatctttta acgcctcacc cgttaaccgt tgttgtaatg ctgtcagttg
    15481 aatatcggga ttcaactgtg tcagtcgctg atggctgacc tgcgatttcg ggcgatcgat
    15541 atcttcagtg gtaaagagga tttgtcgttg cagattgctt aaatgcacat cgtcgtcatc
    15601 tgccagtacc agcgtcccga cgccagcgcc cgccaggtat agcgcagcag gtgtacccag
    15661 cccgccaaga ccgataatca gcacctggct gtcgagcagt ttttgctgcc cgtcaagagc
    15721 gatatcgtcg agcaggattt ggcggctata acgcataaag tcacggtcat tcatcgccaa
    15781 ctcctgcaat ttccagcaac tgtgccgttg ccaaacgcca gtctgcggct tgagtaatgg
    15841 cgcttacgac ggcgatgctg ccgacacccg ttgctatcac cgcaggcgcg cgcgccagac
    15901 tgataccgcc aatcgccacg gtgggataat ccgccaggcg ctcaacatgc cgtgccagct
    15961 gttccagccc ctgcggtgca gaaggcatct gtttggtttg cgtcgggaac acatgtccca
    16021 gcgcgatata agaggggcgt gctgccagcg cgacgtcgat ctccatatcg tcatgggtcg
    16081 aaacgcccag tcgcagacct gccgcgcgga tggcgctgag atcggtggct tgcaaatctt
    16141 cctgccccag atggacgcca tatgcctgat gcttgatcgc cagccgccag taatcgttga
    16201 taaacaatcg cgcgttatag cggcggccca gcgcaattgc cgccacgaca tcggcttcca
    16261 cctcttcatc gcgtcgatct ttgatgcgta gctggagagt acgtacgcct gcatccaaca
    16321 gacgttcgat ccactgtacg ctgtccacca ccgggtacag tcctaaacga aagggtacag
    16381 gaggaaaatc tggctgatac atcacgcttc ctccttacgc aggtagattt ctccgcctct
    16441 ggcgcggaag ttctccgaca tatcagccat tcccacttca atggtttgcg cggcggcgta
    16501 atcgcgcact tcctggctga ttttcatcga gcagaatttc ggcccacaca tggagcaaaa
    16561 atgggcgact ttgccggatt cttgcggcag ggtttcatcg tgataagcgc gggcggtaaa
    16621 cgggtcgagg gccagattaa actggtcttc ccagcgaaat tcgaagcggg ctttcgacat
    16681 ggcgttatcg cgaatttgcg cgcccggatg ccctttcgcc aggtcagcgg cgtgggcggc
    16741 aatcttatag gtgataagcc cctgcttaac atcttcttta ttaggcagac ccagatgctc
    16801 ttttggcgtt acgtaacaga gcatcgcgca gccaaaccag ccaatcatcg ccgcaccaat
    16861 ccccgacgtg aagtggtcat aacccggcgc gatatcggta gttagcggcc ccagagtgta
    16921 aaacggcgct tcgtggcagt gctctaactc ctcggtcata ttgcggcgga tcatctgcat
    16981 cggcacgtgg cctgggcctt caatcatcac ctgtacgtca tattcccagg cgattttggt
    17041 cagttcaccc agcgtatgca gctcggcaaa ttgcgcttca tcgttggcgt cctgaataga
    17101 accagggcgc agaccgtctc ccagcgacag cgaaacgtca taagcggcac agatttcaca
    17161 aatttcgcgg aagtgttgat agaggaaatt ttcctgatga tgggagaggc accatttcgc
    17221 cataatcgaa ccgccgcgag agacgatacc ggtcaggcgt ttcgcggtca tcggcacata
    17281 gcgcagcagt acgcccgcat ggatagtgaa gtaatccaca ccttgctcgg cctgttccag
    17341 cagcgtgtcg cggaacgctt cccaggtaag atcttcggcg atcccgttaa ccttctccag
    17401 cgcctggtag atcggcactg taccgatcgg caccgggctg ttacgcaaaa tccactcgcg
    17461 ggtttcgtga atatagcgac cggtggagag atccatcacc gtatccgctc cccagcgcgt
    17521 ggaccatacc agcttttcca cttcttcttc gatggaagag gtgaccgccg agttgccgat
    17581 attggcgtta acttttacca ggaaattgcg accaataatc atcggctccg attccggatg
    17641 attaatgttg gccgggataa tcgcgcgtcc ggcagcaact tcatcacgga caaattccgc
    17701 agtgatattt tccggcagac gtgcgccaaa gctcattccc ggatgctggt ggcgtaaaac
    17761 ttcgctacgt atgtgctcgc ggcccatatt ctcgcggatg gcgatgaatt ccatttccgg
    17821 tgtgatgatg ccctggcggg cgtagtgcag ttgggtgacg cggcgtcctg ctttggcgcg
    17881 ttttggtgtt agtacgccgc taaaacgcag ctcgtcgagg ccatcatctg ccagccgcgc
    17941 tttagtgtaa tcggaactgc gcacggtaag ttcttcggta tcgccgcgcg catcgatcca
    18001 cggctggcgt agttttgcca gtccttgctg cacgttaatg gcgatctgtg gatcgccata
    18061 cggaccggag gtgtcgtaga ccggaatcgc ttcgttttct tcgtactgcg gttgttcttt
    18121 gctgccgcca attagcgtcg ggctaagctg gatctcacgc atcggcacgc gcacgccggg
    18181 gtgtgtgcca gtgatgtaaa tgcgttttga gttgggaaag gcggtgcctt ccagggtgtc
    18241 gataaaatgt tgggcctggg cgcgttgttc gcggcgggtc agttttgtta cagacatagc
    18301 tcattccaaa aagttaagga cgtggcttgt cagacgacgg atgaagcaag agacgatcgc
    18361 cgcaaaggcg atgcgatagc agattaactc ttgttccctt cgcaggtatt agcctgatca
    18421 ggttccgcgg atcccgaata aacggtctca gccagttaag gcactccgac aagaaattag
    18481 ccccacaaac ggggcgttga atgtaaatta cgcgttaaca gcgcagaact caagcaggat
    18541 gtttgacgcg ggcggcgtca agtaccagca gaatcaactt atcttccagc acaaagcgtg
    18601 cttccagcgc ctcgccgatg tcagataaaa cctgttgaaa ctcaaggtaa ttatcatgat
    18661 cgatggcggt ttccaggctg gaatcgtagt aatccataat ctgttgggta ttggcttcga
    18721 gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt gccttccagc ttatgaagaa
    18781 tgcgctcata aatactgaaa tgtccggcag acaagtaatc gaccaggctc tgacaaaaat
    18841 catcaagggc tttttcgttt agcctcatgt acgattcttt gccaggctta atgccaacca
    18901 gattgtagta agccacgagc agatgcttac gtacatgtag ccagcgatca accagtttgt
    18961 tacttcctct gacgcgttcc gtcaggttat cgagctggtt aagcatgatt gactccgcaa
    19021 gtttgtattc aaaaactgct cagtgagaaa tgtaaaaacc atgttaaaca tgccagtgat
    19081 gcaaaggtag tgcaagagct atggatcgta taattgaaaa attagatcac ggctggtggg
    19141 tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga attgccatat ggcgaagcgg
    19201 caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg cgaatggcaa ggggaacctg
    19261 tttggttagt acaacagcag cggcgtcacg atatggggtc ggtacgtcag gtcattgatc
    19321 tcgatgtagg gctgtttcaa ctggccggac gtggcgtgca actggcggag ttttaccgat
    19381 cgcataaata ctgtggttac tgcgggcatg aaatgtatcc aagcaaaacc gaatgggcga
    19441 tgctgtgcag ccattgccgt gagcgttact acccgcaaat cgccccctgc attattgttg
    19501 ccatccgtcg cgatgattcg atcctcctcg cccagcatac ccgccatcgt aacggtgtcc
    19561 atacagtact tgccggattc gtcgaagtgg gtgaaaccct cgagcaggca gtcgcgcggg
    19621 aagtgatgga agagagcgga attaaagtta aaaacttgcg ttacgtgacc tcccaaccgt
    19681 ggccgtttcc tcagtcgtta atgaccgcgt ttatggcgga atatgacagc ggcgacatcg
    19741 ttatcgaccc gaaagaattg ctcgaggcga actggtatcg ctatgacgat ttgccgttac
    19801 tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga tacggtggcg atgtgtcggg
    19861 cagagtatga gtgatgatac actgaccgcc tgacgcacta aggaacagcc aaaatgaccg
    19921 aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca gcccgttgat gtcactccag
    19981 tatggatgat gcgccaggcg ggtcgctatc taccggaata taaagccacg cgcgcccagg
    20041 cgggcgattt tatgtcgctg tgcaaaaatg ccgagctggc gtgcgaagtg actttgcaac
    20101 cgctgcgtcg ctatccgctg gatgcggcga tcctcttttc cgatatcctc accgtgccag
    20161 acgcgatggg tttagggctc tattttgaag ccggagaagg tccgcgtttt acctcaccag
    20221 tcacctgcaa ggccgacgtc gataaactgc caattccgga cccggaagat gagctgggtt
    20281 acgtgatgaa cgcggtgcgt accattcgtc gcgaactgaa aggcgaagtg ccgctgattg
    20341 gtttttccgg cagcccgtgg acgctggcga cctatatggt ggaaggcggc agcagcaaag
    20401 cgttcaccgt gatcaaaaaa atgatgtatg ccgatccaca ggcgctgcac gcgctactcg
    20461 ataaactggc gaaaagcgtc actttgtatc tgaatgcgca gattaaagcc ggtgctcagg
    20521 ctgtgatgat tttcgacacc tggggcggcg tgcttaccgg gcgcgattat caacagttct
    20581 cgctctatta catgcataaa attgttgatg gtttactgcg tgaaaacgac ggtcgccgcg
    20641 taccggtcac gctgtttacc aaaggcggcg gacagtggct ggaagccatg gcagaaaccg
    20701 gttgcgatgc gctgggcctc gactggacaa cggacatcgc cgatgcacgc cgccgcgtgg
    20761 gcaataaagt cgcgttgcag ggcaatatgg atccgtcgat gctgtacgcg ccgcctgccc
    20821 gcattgaaga agaagtagcg actatacttg caggtttcgg tcacggtgaa ggtcatgtct
    20881 ttaaccttgg tcacggcatt catcaggatg tgccgccaga acatgctggc gtgtttgtgg
    20941 aggcagtgca tcgcctgtct gcgccttatc atctgtaagg agtggttatg gatctcgcgt
    21001 cattacgcgc tcaacaaatc gaactggctt cttctgtgat ccgcgaggat cgactcgata
    21061 aagatccacc ggatctgatc gccggagccg atgtcggatt tgagcagggc ggagaagtga
    21121 cgcgagcggc gatggtgctg ctgaaatacc cctcgcttga gctggtggag tataaagtgg
    21181 cccgcatcgc caccaccatg ccttacattc ctggttttct ctcctttcgt gaatatcctg
    21241 cgctgctggc agcgtgggag atgctgtcac aaaagccgga tttagtgttt gtcgatggtc
    21301 atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggtttg ctggtggatg
    21361 tgccgaccat tggcgtagcg aaaaaacggc tctgcggtaa attcgaaccg ctctccagcg
    21421 aaccgggcgc gctggccccg ctgatggata aaggcgagca gctggcctgg gtctggcgca
    21481 gcaaagcgcg ctgtaaccca ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg
    21541 cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca acgcgctggg
    21601 cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat
    21661 tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa aacccaattc
    21721 atctgcgtct ggagcgcctg gaaagctggc agcacgtcac ttttatggcc tgcttatgcg
    21781 aacgtatgta ccccaattac gccgtattct gtcagcaaac cggcttcggc gatgggcaga
    21841 tttaccgccg cattctcgat ctcatttggg aaacgctgac cgtcaaagac gcaaaagtaa
    21901 atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac gatttcgatc
    21961 tgtacggcgt ttatccggca atcgatgctt gcgtggcgtt aagtgaactg gtccattcgc
    22021 gtttaagtgg cgaaacgctc gaacacgcgg tggaagtgag taagacttcc atcacgaccg
    22081 tggcgatgct ggaaatgact caggctggtc gcgaaatgag cgatgaagag ctcaaagaaa
    22141 acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt ttagctgagt
    22201 gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt gaggcgggtg
    22261 agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt aacgcctgat
    22321 ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa
    22381 aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc gtcgcactcg
    22441 atgcttagca agcgataaac acattgtaag gataacttat gaacaagact caactgattg
    22501 atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct ctggagtcca
    22561 ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg gttggtttcg
    22621 gtaccttcaa agtgaaccac cgcgctgagc gtactggccg caacccgcag accggtaaag
    22681 aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca ctgaaagacg
    22741 cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt tcaccccttt
    22801 tgtctttctg gcgtcgatca ttgatgctgg ctggcgctct gtttctcact gcttgtagtc
    22861 acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag ggcgcggtac
    22921 gcatctggcg aaaagacagc ggcgataatg tgcatcttct tgccgtgttt agcccgtggc
    22981 gcagtggcga tacgacgacg cgagagtatc gctggcaggg cgataacctc acgctcatca
    23041 atatcaatgt ttacagcaaa cccccggtga atattcgcgc gcgttttgac gatcgcggcg
    23101 atctgagctt tatgcaacgc gaatccgatg gggaaaagca gcagctttct aacgaccaaa
    23161 tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc ttacgtcagg
    23221 ggagagtcgt gctgcgccag gggcgctggc atgcgatgga gcagactgtg accacctgcg
    23281 aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc gagcgccgcc
    23341 agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat
    23401 tactgttggt ggcaaactct gatttctgtc gctggcaacc caacgagaaa acgttctgat
    23461 ttaccagtgg tccataccca tatgaccgcc accaccgcag ccgccgtaac ccattccggt
    23521 accgcgcgga atacccgctt cagccatcgc aatatctcgt ttcacccgta actcatctaa
    23581 cgactgacgc aaattctcca tctctttggc gaccgcgtta attttgctgc tatccggtgg
    23641 gttcgcggct aacagggcat tgtattcata acgctttgtt accagttgct gttgcagtgc
    23701 gctgctttga gcgtaaaagt cattatggat tttctgccac gctgtctgtt gttcgctggt
    23761 caaaggcgcg gcattttgct gccacatacc gtgtccgccg tgagcaaatg ccgatgtcga
    23821 tcccatcgcc attgctgaaa gcgccatcat taccagggca attttcgtgt tccgtttcat
    23881 ggttaatcct ccagtggttg tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa
    23941 cgctgatatg acgggtaatc tggcatgata aacgagtaaa aatgactcgc ctgctgcggg
    24001 tagcgagtca tttttactca ttgaaactta agcctttgtg ttacagcgca gggtaagcgc
    24061 tgataaaaga tggcatgatt tctgctgtca gaaagggatg agcaggcaaa gaagaagatg
    24121 cgttttatgc aacgttctaa agactcctta gctaaatggt taagcgcgat cctccccgtg
    24181 gtcattgttg ggctggtggg gctgtttgcg gtgacagtga ttcgcgatta cgggcgcgag
    24241 actgcagccg ccagacaaac gctgctggaa aaaggcagtg tacttatccg tgctctcgaa
    24301 tccggctcgc gcgtcggcat ggggatgcgc atgcatcatg cgcagcagca ggcattactg
    24361 gaagaaatgg ccgggcagcc tggtgtacgt tggtttgcgg tcacggatga acaaggaaca
    24421 atcgtgatgc atagcaactc cggcatggtg ggaaaacagc tttattcccc gcaggaaatg
    24481 cagcagttac atccgggaga tgaagaagcg tggcggcgga tcgatagcgc agacggcgag
    24541 cctgttctgg aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg
    24601 cgccatatgc agcagtatgc cgcgacacca caagcaattt ttatcgcttt cgacgccagt
    24661 aacattgtga gtgccgaaga tcgtgagcag agaaacaccc tgattatcct cttcgccctg
    24721 gcgacggtct tgctggcaag cgtgttgtca ttcttctggt atcgccgata tctgcgctcg
    24781 ggccagctgt tgcaggatga aatgaagcgc aaagagaagc tggtggcact ggggcatctt
    24841 gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg gctggcgaaa
    24901 tactttgccg aacgcgcgcc agcaggggga gaagcgcatc aactggcgca ggtgatggcg
    24961 aaagaagccg accgtttaaa ccgcgtggta agcgagttgc tggaactggt taagccaacg
    25021 catctggctt tgcaggcggt ggatctcaac acgctgatta accactcatt acagctggta
    25081 agccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga cacattaccg
    25141 gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct
    25201 attcaggcga ttggtcagca tggcgtgatt agcgtgacgg ccagcgaaag cggcacgggt
    25261 gtgaaaatca gcgttaccga cagcggtaag ggaattgcgg cggatcagct tgaagccatc
    25321 ttcaccccgt atttcaccac gaaggccgaa ggcaccggac tggggctggc ggtcgtgcat
    25381 aatattgttg aacaacacgg tggtacaatt caggtcgcaa gccaggtggg aaaaggcgca
    25441 acgttcaccc tctggcttcc ggtcactatt acgcgtaagg acccacaagg atgacgcacg
    25501 ataatatcga tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt
    25561 tgctgcgcgg ctggggctat aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc
    25621 aggtgcggga acaggttttt gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg
    25681 gcattgccac gctaaaagag atcaaagcgc taaatccggc gattccggta ctgattatga
    25741 ccgcatactc cagcgtcgag acggcggtag aggcactgaa aaccggggcg ctggattatc
    25801 tcatcaagcc gctggatttc gataacctgc aggcgacgct ggaaaaggcg ctcgcacata
    25861 cgcacagtgt tgacgctgaa acgcctgcgg tgtctgccag ccagttcggt atggtaggta
    25921 aaagcccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca
    25981 cagtgctgat ccacggcgat tccggcaccg gtaaagagct ggtcgcaagg gcgattcacg
    26041 ccagtagcgc acggagtgaa aaaccactgg taacgctcaa ctgtgcggca ctcaacgaat
    26101 ccttgctgga atctgaattg ttcggtcacg aaaaaggggc gtttaccgga gccgataaac
    26161 gccgggaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg
    26221 atatctcgcc gatgatgcag gttcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc
    26281 gtgtcggcag caaccagacc atctcggttg atgtccggct gattgcggcg actcatcgcg
    26341 atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaacg
    26401 tggtggcgat tgaagttccg tcgctgcgtc agcggcggga agatattcct ctgctggcaa
    26461 accattttct tcagcgcttt gccgagcgta atcgtaaggc ggtaaaaggt tttacgcccc
    26521 aggcgatgga tctgctgatt cactacgact ggccgggaaa tattcgtgaa ctggaaaaca
    26581 cggtggaacg ggcggtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc
    26641 tggcgattgc gagtacgccg atcccgctgg tacaaagtca ggatattcag ccgctggtgg
    26701 aagtcgaaaa agaggtgatt ctggcggcgc tggagaaaac gggcggcaac aaaaccgaag
    26761 ccgcccgcca gttagggatc acgcgcaaaa cgctattggc aaaactgtcg cgttagttct
    26821 gctcgcgttc gatagcgcgc cagccgatat ctttccggca gaagcagtcg tcccaatgga
    26881 tatcggtcat taaggcataa gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg
    26941 taacgcacag tacgcgcccg ccgctggtca ctacctgctc gtcatccgct agtttcgtgc
    27001 ccgcgtggaa cactttgccg tctgccactt cttccagcgg caggccgtgg atcacatcgc
    27061 cggtgcggta atcgcccgga tatccacccg cagccatcac cacgccgaga gaagcgcgtt
    27121 catcccactc ggacgttttc tcgtccagct tgccttcaca ggccgccagg cagagctcaa
    27181 ccagatcgga cttcatgcgc agcataatcg gctgggtttc tggatcgcca aagcggcagt
    27241 taaattcaat aaccttcgga ttgccctgtt tgtcgatcat caggcccgcg tagagaaaac
    27301 cagtgtaggt gttgccttct gccgccatgc ctttcacggt tggccagatg attcgttcca
    27361 tcgtgcgctg atgaacttca tcggttacta ccggcgcggg ggagtaagcg cccatcccgc
    27421 ccgtgtttgg tccggtatct ttatcgccta cgcgtttgtg atcctggctg gtagccatcg
    27481 gcagcacatg ctcgccgtcc accatcacga taaagctcgc ttcttcgcca tcgaggaact
    27541 cttcgataac gatgcgatga cccgcgtcgc caaacgcgtt gcccgccagc atatcgtgaa
    27601 cagctgcttc agcttcttcc agcgtcatcg ccacgataac gcctttcccg gctgccagac
    27661 cgtcagcttt aatgacgatt ggcgcgcctt tctcacgcag atacgccagc gcaggttcta
    27721 cctcggtgaa gttctggtat tccgctgtgg gaatgttatg gcgggccaga aagtctttgg
    27781 tgaatgcttt agaaccttcc agttgtgcgg cacctgcggt tgggccgaag attttcagcc
    27841 cggcggcgcg gaaggtatcg actacgcctt tcaccagcgg cgcttccggg ccgacgatgg
    27901 ttagatcgat cttttcgttt tgcgcgaaat ccagcagcgc cgggatatcg gttacgccga
    27961 tagcgacgtt ttgcagtgtg ggttccagcg cagtgcctgc attacccgga gcaacaaaaa
    28021 cagtttcaac cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt
    28081 taccaatcac taatactttc atctgtcgct ccattaatgg cggaagtggc gcatgtcggt
    28141 gaagagcatc gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggataga
    28201 accgccaggc tggattacgc aggtcacacc cgcagcggcg gcggcatcaa taccgtcgcg
    28261 gaacgggaag aacgcgtcag aagccatcga ggaacctttc acttccaggc cttcatcggc
    28321 cgctttaata ccggcgattt ttgcggagta cacgcggctc atctggcccg cgccaatgcc
    28381 gatggtcata ttgtttttgg catagacaat ggcgttggat ttcacgaact tcgccacttt
    28441 ccagcagaac agcgcatcac gcagttcctg ttcggtcggc tgacgtttgg tgacgacgcg
    28501 cagttcttct gcaccgacca tgcccaggtc gcgatcctga accagcagac cgccgttaac
    28561 acgtttgaaa tcaagacccg gaacacgctc gccccactga ccgcaggtca ggacgcgtac
    28621 gttctgtttg gcggcggtga ttttcagggc ttcttcgctg gcggacggcg caataatcac
    28681 ttcaacaaac tgacgagaaa tgatggcctg cgcggtttcc gcatccagct cgcggttaaa
    28741 ggcgataatg ccgccgaatg cggaggtcgg gtcggttttg tacgcgcgat cgtaagcatc
    28801 aagaatggaa ttgctgatag ccacgccgca cgggttggcg tgcttcacaa tcacacatgc
    28861 tggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata
    28921 agagagggcc ttaccctgaa cctgggttgc ggtggcaaca gaggcttctt tcacgttctc
    28981 ttctatatag aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt
    29041 aatgaagttc aggttcagcg tgcgtgggaa gcgaccggcg gcttctttgc tttcaccgtg
    29101 gtaagccgga accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcggtatg
    29161 ttcaaaggct ttgatagcga gatcgaaacg ggttgcaagc agaagcgatc cttcgttggc
    29221 atccatctct ttaatgatgg cgtcgtagtc gctgctcttc accacgatgg cgacatcttt
    29281 atggttcttg gcggcagagc gcaccatcgt cgggccgccg atatcgatgt tctcaaccgc
    29341 atcttccagc gagcaacctt cacgggcaac ggtctgggcg aacggataca ggttaacaac
    29401 caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg
    29461 gcccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc
    29521 cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg
    29581 ggctgtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc
    29641 gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg
    29701 catggtaaat cccctggatt tgactattac agagagcgtt agctgaattt ttcgcgaaaa
    29761 actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt
    29821 ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtggctac
    29881 cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta
    29941 taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttatgcaat ttttctattg
    30001 cgacctgcgg agaactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    30061 acacaaacag ccggttcggt tgaagagaaa aatcctgaaa ttcagggttg actctgaaag
    30121 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagccgcgtc gca
//
LOCUS       DAZGTJ010000060        30166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000060 DAZGTJ010000000
VERSION     DAZGTJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.060"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..557)
                     /locus_tag="ACHK8E_003226"
     CDS             complement(<1..557)
                     /locus_tag="ACHK8E_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345451.1"
                     /translation="MNQLKSMNINKLLLDVDNPRFPTSAENQRDAIAKMLDLQYDRIY
                     RLAKDIASKGLDPSENILVYPSEEESGFFVVAEGNRRVTALKLLHSPRLAPTEKARKA
                     FEKLKITQLKDINVIDNCVLFDDDEYEHWVNLKHTGQNAGVGRVEWTATEKARHLARM
                     GKQSFGNQILTFIEFNTDLYKEIIA"
     gene            complement(914..3199)
                     /locus_tag="ACHK8E_003227"
     CDS             complement(914..3199)
                     /locus_tag="ACHK8E_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0345452.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAITGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQTHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIRITRTTDWRIVPKVPVVQPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKYGLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMARI"
     gene            complement(3189..3464)
                     /locus_tag="ACHK8E_003228"
     CDS             complement(3189..3464)
                     /locus_tag="ACHK8E_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP0345453.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3461..3685)
                     /locus_tag="ACHK8E_003229"
     CDS             complement(3461..3685)
                     /locus_tag="ACHK8E_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0345454.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3685..3987)
                     /locus_tag="ACHK8E_003230"
     CDS             complement(3685..3987)
                     /locus_tag="ACHK8E_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP0345455.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3987..4211)
                     /locus_tag="ACHK8E_003231"
     CDS             complement(3987..4211)
                     /locus_tag="ACHK8E_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP0345456.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4275..4775)
                     /locus_tag="ACHK8E_003232"
     CDS             complement(4275..4775)
                     /locus_tag="ACHK8E_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFP0345457.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4772..4942)
                     /locus_tag="ACHK8E_003233"
     CDS             complement(4772..4942)
                     /locus_tag="ACHK8E_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345458.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4953..5228)
                     /locus_tag="ACHK8E_003234"
     CDS             complement(4953..5228)
                     /locus_tag="ACHK8E_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFP0345459.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            5350..5649
                     /locus_tag="ACHK8E_003235"
     CDS             5350..5649
                     /locus_tag="ACHK8E_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0345460.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5765..6778
                     /locus_tag="ACHK8E_003236"
     CDS             5765..6778
                     /locus_tag="ACHK8E_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0345461.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(7055..7372)
                     /gene="yegR"
                     /locus_tag="ACHK8E_003237"
     CDS             complement(7055..7372)
                     /gene="yegR"
                     /locus_tag="ACHK8E_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP0345462.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            7787..8686
                     /gene="yegS"
                     /locus_tag="ACHK8E_003238"
     CDS             7787..8686
                     /gene="yegS"
                     /locus_tag="ACHK8E_003238"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP0345463.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(8768..9541)
                     /locus_tag="ACHK8E_003239"
     CDS             complement(8768..9541)
                     /locus_tag="ACHK8E_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0345464.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(9647..10687)
                     /gene="gatD"
                     /locus_tag="ACHK8E_003240"
     CDS             complement(9647..10687)
                     /gene="gatD"
                     /locus_tag="ACHK8E_003240"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0345465.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(10735..12090)
                     /locus_tag="ACHK8E_003241"
     CDS             complement(10735..12090)
                     /locus_tag="ACHK8E_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0345466.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(12094..12378)
                     /gene="gatB"
                     /locus_tag="ACHK8E_003242"
     CDS             complement(12094..12378)
                     /gene="gatB"
                     /locus_tag="ACHK8E_003242"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP0345467.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(12409..12861)
                     /gene="gatA"
                     /locus_tag="ACHK8E_003243"
     CDS             complement(12409..12861)
                     /gene="gatA"
                     /locus_tag="ACHK8E_003243"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP0345468.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(12871..14133)
                     /gene="gatZ"
                     /locus_tag="ACHK8E_003244"
     CDS             complement(12871..14133)
                     /gene="gatZ"
                     /locus_tag="ACHK8E_003244"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP0345469.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(14162..15016)
                     /gene="gatY"
                     /locus_tag="ACHK8E_003245"
     CDS             complement(14162..15016)
                     /gene="gatY"
                     /locus_tag="ACHK8E_003245"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP0345470.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(15245..16297)
                     /gene="fbaB"
                     /locus_tag="ACHK8E_003246"
     CDS             complement(15245..16297)
                     /gene="fbaB"
                     /locus_tag="ACHK8E_003246"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP0345471.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            16554..17831
                     /gene="yegT"
                     /locus_tag="ACHK8E_003247"
     CDS             16554..17831
                     /gene="yegT"
                     /locus_tag="ACHK8E_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP0345472.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17828..18832
                     /gene="yegU"
                     /locus_tag="ACHK8E_003248"
     CDS             17828..18832
                     /gene="yegU"
                     /locus_tag="ACHK8E_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0345473.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            18829..19794
                     /gene="yegV"
                     /locus_tag="ACHK8E_003249"
     CDS             18829..19794
                     /gene="yegV"
                     /locus_tag="ACHK8E_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0345474.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(19768..20514)
                     /gene="yegW"
                     /locus_tag="ACHK8E_003250"
     CDS             complement(19768..20514)
                     /gene="yegW"
                     /locus_tag="ACHK8E_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0345475.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(20566..21384)
                     /gene="yegX"
                     /locus_tag="ACHK8E_003251"
     CDS             complement(20566..21384)
                     /gene="yegX"
                     /locus_tag="ACHK8E_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP0345476.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(21449..22249)
                     /gene="thiD"
                     /locus_tag="ACHK8E_003252"
     CDS             complement(21449..22249)
                     /gene="thiD"
                     /locus_tag="ACHK8E_003252"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP0345477.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(22246..23034)
                     /gene="thiM"
                     /locus_tag="ACHK8E_003253"
     CDS             complement(22246..23034)
                     /gene="thiM"
                     /locus_tag="ACHK8E_003253"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP0345478.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(23069..23165)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23257..23529)
                     /gene="rcnR"
                     /locus_tag="ACHK8E_003254"
     CDS             complement(23257..23529)
                     /gene="rcnR"
                     /locus_tag="ACHK8E_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP0345479.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            23651..24475
                     /gene="rcnA"
                     /locus_tag="ACHK8E_003255"
     CDS             23651..24475
                     /gene="rcnA"
                     /locus_tag="ACHK8E_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP0345480.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            24694..25032
                     /gene="rcnB"
                     /locus_tag="ACHK8E_003256"
     CDS             24694..25032
                     /gene="rcnB"
                     /locus_tag="ACHK8E_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP0345481.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(25114..26148)
                     /gene="yehA"
                     /locus_tag="ACHK8E_003257"
     CDS             complement(25114..26148)
                     /gene="yehA"
                     /locus_tag="ACHK8E_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP0345482.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLINLESIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAEDNGVGFML
                     ESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNKVKSGALQ
                     ATALINVKYD"
     gene            complement(26164..28644)
                     /gene="yehB"
                     /locus_tag="ACHK8E_003258"
     CDS             complement(26164..28644)
                     /gene="yehB"
                     /locus_tag="ACHK8E_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0345483.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            complement(28660..29334)
                     /gene="yehC"
                     /locus_tag="ACHK8E_003259"
     CDS             complement(28660..29334)
                     /gene="yehC"
                     /locus_tag="ACHK8E_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0345484.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(29415..29957)
                     /gene="yehD"
                     /locus_tag="ACHK8E_003260"
     CDS             complement(29415..29957)
                     /gene="yehD"
                     /locus_tag="ACHK8E_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345485.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         7780 a         7620 c         6769 g         7997 t
ORIGIN      
        1 ttagcaatta tctctttata taaatcagta ttgaactcta taaatgtaag tatttgatta
       61 ccaaatgact gcttacccat cctagccaga tgtcgtgctt tttctgttgc agtccattca
      121 actcttccaa ctccagcatt ttgaccagta tgctttaaat tgacccaatg ctcatattca
      181 tcatcgtcaa aaagaacgca gttatctata acattaatat ctttaagctg agtaatctta
      241 agtttttcaa aagctttccg tgctttttct gttggtgcaa gtctaggaga gtgtaaaagt
      301 ttaagggctg taactcgacg attaccttca gcaacgacga aaaacccaga ttcctcttca
      361 ctaggataaa ctaatatgtt ttcagacgga tctaaacctt tcgaagcaat atctttagcc
      421 aagcgataaa tgcggtcgta ctgcaagtca agcatcttgg caatagcatc acgctgattt
      481 tctgctgacg tcgggaaacg agggttatca acatccaaaa gtaacttatt gatattcatt
      541 gattttagtt gattcatcaa catatcctct gttgaatctt tatgataaaa attaattatt
      601 tttatcataa atgatagcgt aaacatagtc ttatgaacga catcagccct gaagcttcaa
      661 aactatcatt tacgtttgcg aggcaccatc tatcacattg atttataata agtatcttta
      721 tcttcactgc attgctctca aaaaatcaca tagatggaaa ttccccgttg acacactccc
      781 aagtgatgca gatacatttc aagtgcttca tacacatgat aacggctatc cccctaaatt
      841 ctgtgcgttt cttttccctc cagcacaagg ttgaaatatc ttgcgtgcag ctttactttg
      901 tgaatctttg tcattatatc cgcgccatta ctgttgagaa tcccggccac tcatcagcga
      961 ccgggtacgt gaattttttc ccgtcataat ttacggtcgc gccacgcgcc agcgcctcaa
     1021 gctcccatcg ctgcggcctg ataccgttct gagcaaggtc aacgcggata cgggtaattt
     1081 gcattctttc cgaccgggtc agtctggccg atggtgcaat ttcatgtggt tttaacgggc
     1141 ttccgtttct ctgctgacga tttggggttc tcagcccgta ttttaatgcg cccctgagcg
     1201 ccctcacgac ctccgggtca ttccattcga taacaccgtc atcaaccaga ttaagcactg
     1261 ctgcggcgtg ttcagaaggt gtgggagccg gtaacgaagt atcaccaccg gtgagctttc
     1321 cacagttatt gacaggactc cgaggcgcgg cgatgccgct ttttaaagtc aaaggctgaa
     1381 cgaccggaac tttcggcaca atgcgccagt ccgtcgttct ggtgatacga atatgacgcg
     1441 cgccgagatg cggcgcgtaa atgccgacca ctctctcgac ctcttcctcg tactcgttaa
     1501 cgtcatccga ggggctacgg gcgaccctga cagtctggca atcgcgcggg acatttgccc
     1561 caccctgcgc gctgatatac agcgcaaaat cgccactgtc tgcggcagcg cgtgcagcct
     1621 cgacgcgttc gtcaaactca tcagcaatgc tgacgccgcg aggcaatttg cgtagttcac
     1681 ggtaagcccc cattgtcggc aggccaaccg ttttaaattg aggaatgcgc cacgttgacg
     1741 cccatgcggt aacagccgcg gcagtgtctt tcagcggcct gccggtgtcg ttatcgagct
     1801 gaccatccag tgcatagcca tcgatgtttt ttgaaatgta tttcgcgata tatcccgcag
     1861 caccgccccg gttaaggtgt tttgcctgaa aacggtttcg cgcggctcct ctttcgtcgc
     1921 catcctcttt gagcgcgtag cgacgcatga tttcgataat ctgattacgc tggcgtggat
     1981 tacaaaaaag catcatatgc cagtgcggcg ttccgtcgtg gtgtggctcg acgacacgca
     2041 aaccgtagac ctgtaaatca ttatccttga atgccgtgcg catcaggctc cagatgcggc
     2101 agagataacg ctgcgcatcc tttggattaa atgcctcatc gttccagccg tgattaagct
     2161 gcacggtttt actttcgcct tttccgacct gacgtgtcgg gtgatacttt gacggtgcgg
     2221 tcagcgtgat aaacatcccc acatcgccct ctgcggcggc gtaacgctca ataccggcaa
     2281 tggtgttcat cagctccatc cggcgaattt caggattaga aatactgccc atcaccttac
     2341 tgataaggtc gatgcgctcg ccggtttccc tgttttcgag gtcacacgat ttaagaaatt
     2401 ccagatttgc ctggcggcgc gcacgcacat cacgaatggc atgtttactg gcataaggag
     2461 aacggtcttt attgacctcc ccgacagcaa tcagtaacgc ctcatgccag cgcatacgct
     2521 ggcctttaag ctgactaatc caccactcat cgttaaacag acgggcaatg gcagaatatg
     2581 cctgcctcgt ggtcatctgc cctttacggt attttttcca gtagagcggg gaaatattga
     2641 aagcacgtgc agcgccagcg acatgaccat acaggtgagt ctgcgcctca tccgtaaaca
     2701 gcgattcttt ttcgccatgt gcatccaccc aggcatcgca gagttcctca tacatcatga
     2761 aaagctgcga tgagatacgg gcggcaaact ttttcagctc cttgtcattc attccaggca
     2821 ggcgcgcata atggtcacgc tctgccagaa acagcaacga cgcgtcggtg ttcatttcat
     2881 ggcgctgatt cacgcgctca atgcgcggcc ataaacgacg ctgaaaagtg gatgtgagga
     2941 aataaaaccc gtgcaccggg cttttattgc gccggatgta gtcataacgt gaagtaaaca
     3001 gcgagcgcaa aaagtaaggc aggcggttaa tcgtggataa aacaccttgc acctgacgca
     3061 tctcgtcacg tgtaaggggt ctttcgcgcc cgacagcctc gcgtggcgcg ttccatgcat
     3121 aagcaccggt aatcgcctta ccggtgcctg cggcaaatgc tgacggaggg acaaaacgcc
     3181 cggaggcttt aacggccata tgagccaaaa gcctcagaac aacgcttgct gagttgctca
     3241 acctgcgcgt ttaaatcagc aaaagacttt gcgcttccgg tcagaatatc gtgatgcatc
     3301 aggccggaaa cgagctggct taatttcggg taataaccaa ccaccgccag ccattcctgc
     3361 ccggcgtttt taccgctttc cgctctcttt ttctcgtgga gaataaactg aaagctgtca
     3421 ctggtaacga cataacgttc gccaatttcg atacgaatac tcatgccatt ctccggtaat
     3481 gcttgttttt tgcttcaaag actgactggc aggaaacaca acgcgtggct gacggataag
     3541 ctgcacgacg ggcagcaggt attggcgcgt cacactcttc gcaaaccagc gcagaaacac
     3601 cgcaatgttt tacccttgcc gcgttaatct ggcgctccag taattcagcc tgttgttcct
     3661 gaataaaatc tatgttgtcc ggcattacca gctccttttg tcgttcagct tcttaaattc
     3721 atcagcgcaa tagctggcga tttctgtcgt taattttgtc agttcatcca cggatgaaat
     3781 ttgcttgtgg aatacagcac gtttaacaag taaattgacc acatcagaca ggaggtttaa
     3841 ttcattctga taaatcgcga taacagattc agttatgtcg cgcttttctt tatcaagaca
     3901 aagttgaata agagacaaat caccattttc cataacggcg atttttaagg cgttattcag
     3961 taatacaact gaatgagaac aggacatcaa agtacctccc cgcgagacaa tccgatattg
     4021 tgaaattttt ccgactcctg actgagcagc tcgactatct ccacgcggga taactccgcc
     4081 tttgtgatgt ggcgaatcat ggcgtcaaga tgagaagaaa agcgcgtagc tgcatctgcc
     4141 tgtgcttcgg ttctggcctg ttgcagcagt aatgcgtatt taccgcactg attttcagaa
     4201 actgtatgca tgactttctc caggcaaaaa gaagccccgc acaattaagt gcgttaaaaa
     4261 ctctggttaa ttacttaatg cagatattgc tctggtttta ccgatgtcag aattgtcggt
     4321 gcatactcaa acagactgaa taattcacgt aatgcacgga ataaagcatc acgccagtaa
     4381 catgattctt cattaattcg ccagtacggc tggttgaatt ctttttctgt cagtcgtgcg
     4441 tgcataaata aagtacgacg ctggctgact gttaaaaaac taatatatgc atactcactt
     4501 gcaccaacct gacggcgttt tgagaatgcc ccacgcaatt catcaattgc acataccagt
     4561 cgttcacgtt cgacgtcgtt catttcttca aaacgcatcg ttgcgtgacg ctgttttaac
     4621 tgcgcatgaa agcaaaccgt tagccgttcg cgttccatca tctgattata ataatcgcat
     4681 gtctcctgcc agcgagggac ggccagatgc ttaccaatta tccggcgcat agttgctggc
     4741 tgtttttcaa cgagattgag cgtcatcact gtcatttcca taccctccgg cttttcagaa
     4801 aggtcagagc cttttttaac ggactctgtt ttttggtgcg gataatgatt cccttacgcc
     4861 ccttaccgtg ggtgatggtg aagtcaatcg ccctggggct ttcgttacgc aataactgag
     4921 caatacaacg aggctcattc atacggttct ccttaacgtg gttcaccgag acctaaccac
     4981 atcaaccagc cgtcacgaat ctctttagga cggctttcat aagccagttt tagtccgtta
     5041 ttccatgccg gaaggtatac ccaatattca ccagcacgcc ccgatactga ctgagggtca
     5101 gtaatctcaa taactggtaa tttccctttc tcaatcatac cccttacagc tcttggagtt
     5161 ttaccaatga gttttgcaaa ctcctgataa ggcacggcat cagtcacgct tacaagctgt
     5221 ctattcatct gctacgattc tcccttagtg cttctaatgg ctcctaatgg ctaattattg
     5281 cctaaaagga taactccaga agcacaacat ttcacactat cagcaagaaa ttacgcaatc
     5341 ggagtaatta tgtcaataga cgtttcggag aagttgaagc taatccgtga atctgaaagg
     5401 ttaaaccgta aagaattcag tgaattaact ggtgtagcct acagctcact ttcgagctat
     5461 gagagccggt caaaaaacgc tggagttgaa gccataatga aggtcttaca acatcctaga
     5521 tttactaaat atactttgtg gttcatgact gatcaggtag ctccagaagc cgggcaaatt
     5581 gcgcccgctc tcgcacactt tgggcaaaac gaaacaacgt cgccccactc cggtcaaaag
     5641 actggttaac aatttatcgt gaatatattc atcacaagtg cctactattg gtggctaaat
     5701 ttcagccacc acgaaaaaag cgattagtag tagcaaaaaa aagtaccact cggagggttt
     5761 tctgatggca atcaaaaaac tcgatgatgg tcgatatgaa gtggacatcc gccctactgg
     5821 acgtaacgga aaacgcatcc gtaggaagtt tgataagaaa agcgaagctg tcgctttcga
     5881 aaaatacacg ttgtacaacc accacaataa agaatggcta tcaaaaccaa cagacaaacg
     5941 acgtctgtcg gaactgacac agatctggtg ggatttaaag ggtaaacacg aagagcatgg
     6001 gaaatctaat cttggaaaaa ttgaaatctt cacaaaaata acgaatgacc catgcgcatt
     6061 tcaaatcacg aaatccctta tcagccagta ctgcgccacc cgaagaagtc agggtattaa
     6121 accttcgagt atcaatcgtg atttaacatg tattagcggc atgtttacag ccctgattga
     6181 agcggagtta ttctttggtg agcaccctat cagagggaca aagaggctta aggaggaaaa
     6241 accagaaaca ggctatctca cacaggaaga aattgcctta ctgcttgctg ctcttgacgg
     6301 cgacaacaaa aagattgcga ttctttgcct gagcactgga gcacgttggg gagaagcagc
     6361 tcgtttgaaa gcagaaaata tcatccataa ccgcgtcacg tttgttaaaa cgaaaacaaa
     6421 caaaccacgc accgtcccga tctcagaggc tgttgccaaa atgatcgcgg ataacaaacg
     6481 aggtttttta ttccctgatg ctgattaccc tcgcttcaga cgaacaatga aagcaataaa
     6541 accggatttg ccaatggggc aagccacaca tgcactaagg cacagctttg ccactcattt
     6601 catgattaat ggaggaagta ttatcacgct acaacggata ctaggtcaca cgcggattga
     6661 gcaaactatg gtttacgctc attttgcgcc agagtacctt caggacgcca tttctcttaa
     6721 tccgctaaga ggtggtactg aggccgagag tgtccacaca gtgtccacag tagagtaacg
     6781 tttaagggct ttcagtggta atttatgccg ctcaaacccg cattgtaccg ttgaaagccc
     6841 ctactggtga caccctaaat ctcccttaca cgggcttatt ttttatacat aaaccataga
     6901 gacggattga tcccaggtca ccgtcttcca ttgaccacat cgatagaatc tcccttcata
     6961 gcacgatgcc tttcacttat tggcatcgtg ctcgcacagg ttccggttac gcacagccag
     7021 aacgcgcatg tttgatgctt accaaaaaat attctcactc tccacatttg aatgtcagac
     7081 gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc atttttttgc
     7141 cctgagtaac acagaaatga actgcatcat tttttactat attttctgca ccagatattc
     7201 tacccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa
     7261 tattactttt atatgcaaga ccaaatttac cgatacttgt ctcatcatta tgtacagcac
     7321 acccagacat aagaaaaaca ctaattaatg atatagcagc tatctttttc atctcacctt
     7381 cccccattaa ataccaacga cacgctctgg tgtttaaata taataatggc atgattatta
     7441 taattgaata ggattataat aaatgttctg tacaacattt cctacataag taggaattac
     7501 atacacggat gcccttccag gtaaccccat gggggggtta atatattttt aatagtatat
     7561 tgaagatgcc actgtttagt tgaatattag gtatatgttc ttttttgaaa tttaccggtg
     7621 gctaccgtta atattcgctg ttcccattgc aagctcctgg tggtaaccac tgaatcctcc
     7681 atacttgaac tgacttttta tcctccgact ttcatcctgt tctgactcca ccttttcttt
     7741 tctgctctac actatctaca gtccaatcat aaaggcacat acgatcatgg cagaatttcc
     7801 cgccagctta ctgattctta atggcaaaag tactgacaat ctacccttgc gcgaagcaat
     7861 tatgctgttg cgtgaggaag gaatgacgat ccatgtgcgg gtcacctggg agaaaggcga
     7921 tgccgcacga tatgtagagg aggcacggaa gttgggcgtc gcaacggtga ttgccggtgg
     7981 tggcgatggc accattaatg aagtttctac ggcgttgatt cagtgtgagg gggatgacat
     8041 acccgcgctg ggaattttac cattaggaac cgccaatgat tttgccacca gtgtagggat
     8101 tcctgaggca ctggataagg cgctgaaact ggcaattgcc ggtaacgcca ttgcgataga
     8161 tatggcgcag gtcaataaac aaacctgttt tattaatatg gcgacaggcg ggtttgggac
     8221 gcgtattacc acagaaacgc cggaaaaatt aaaagccgcg ctgggtggcg tctcttacat
     8281 cattcatggc ttaatgcgca tggacactct gcaaccggac cgttgtgaaa tccgcggtga
     8341 aaactttcac tggcaaggtg acgccctggt cattggtatt ggtaacgggc gtcaggccgg
     8401 tggcggtcaa caattgtgcc cgaacgcgtt aattaacgat ggcttgctgc aactgcgcat
     8461 ttttaccggc gatgaaatta ttccgactct cgtatcaacc ttaaaatctg acgaagataa
     8521 cccgaatatt atcgaaggag cttcgtcgtg gtttgatata caagccccac acgaaatcac
     8581 ttttaatctt gatggcgaac cgttgagtgg gcaaaacttc catattgaaa tacttccggc
     8641 ggcgttgcgt tgtcgattac caccagattg tccattgttg cgttaatcaa actattctct
     8701 gttgaaccag agagaatccg taatgccgga tgcggtgtaa acaccttatc cggcatacag
     8761 aacaatacta caccgtaatt aattttaccc gtgtcttctg caatgccagt ttatccacct
     8821 cagacacagc atcatcggta ataataatgt caattttttc gattggcagt acctgattaa
     8881 aaccgcgacg attaaatttc gatgaatcga gaacggcaat cactttattc gccgctgtca
     8941 ccatcgcacc gctaacggaa taaccttcat taaaggtcgt aataccatta accgcatcaa
     9001 taccatcagc accgacaaac attaaatcgg cattaatatc ctgcaatgaa cgctcagcaa
     9061 tggaaccatg catcgagcgc gttttatggc gcaccgtacc accacaaacc accaaagtga
     9121 tgtctttatt ttcggaaagg gcaaacgcag ccgggagact gttggtaatc accgtgatat
     9181 ttttggcggt cattaatcct tcggcgataa gcattgtcgt acttccgctg tcgagaataa
     9241 cggtcatccc ttcgtggatc attttgactg ccgcctgcgc tattctgttt ttcggcgcgc
     9301 tggcgagctg aaatcgctct ttaaacccga cttcctgggt ctcggtttcg ctattaccgg
     9361 acattatttt cgccgcaccg ccatgaaagc gcgtaaccac gcctttttgt tcgagaaagc
     9421 gcaaatcggc acggattgtc gcttccgagg cagcaaatac tcccgccaga tcctgaacaa
     9481 gcacggttcc ctgttcattc actaattgga tgatcttatt ccttcgctcg aatgaattca
     9541 tcgtcattta cccatattca attgtggcta gtgtaaacga agggggacca ttggtgaatg
     9601 atctgttttg cacaataccg ggcctgcatc acgctggccc gcggtttcag ggaatgagca
     9661 acactttgcc cggcatggca ttacgagcga tgtcacgcac cgcctgggcg aagctttcaa
     9721 agcttccacg gtgagcgatt aatggctcca ggcttaactt acgttctgtc agcaaccggc
     9781 tcgccgtttc ccactcctgc cccggccaag ggctggagta gttcatccaa ctgccgataa
     9841 ccgtcagctc tttacgcaat attttgccaa acgttgcaga tgttaaatgc agatcctgat
     9901 gcaacgtgcc caccagcgcc agttgggcat gaggaccggc aatctctacc gccagttcga
     9961 cggtttgcgg gacgccagcc gtctcgagga taagctgatt aaagcgcagc tcgcgtaaaa
    10021 cgccctgtat ttgcggcgcg ctcatttcac ggctgttaaa tgtttgcatc gctccgaaag
    10081 attttgccag tgccagtttt tctgagctaa tgtcgatagc cgtcacactc tttgctccca
    10141 gcgcgacagc gcactgaatt gccagcaggc caatggttcc ggcaccaata ataataacgt
    10201 ttttattctc acatccttgc gctaaatgga acgcatgcag gccaacggta atcggttcaa
    10261 taaaagcgcc atcctcaata ggcatatccg caggtagtgc aaagacattg tttcgcttaa
    10321 cgacaatata ttcggcaaat ccaccatcac gccgcgagcc aataaaatca tattttgcgc
    10381 actgggaata aaaccctttc aaacactctg gacaagtaaa acagggtaat aacggcacac
    10441 aggcaaccac atcgccaggg tgtaaatcat caacaccgga tcccaccgca tcaatatagc
    10501 cgctaaattc atgacctaac gttattggat aataatgtgc accattttta aatatcctgg
    10561 gtaaatcgga accacataag cccgagctgg caatttttac ccgcacctca tcctgatgtt
    10621 taatttcagg aatgacgctt tctgcaacgc gcacgatacc atcagtatca ttcaccactg
    10681 atttcataaa aactcctgat tattaagggg gataacctcc ccctcaggta aaaattattc
    10741 tgcgagaacg gctttctctt gtttaataaa gccacgcgct ctacgccagg tcatgaaaat
    10801 accggtaaga taaatggcac cgataataat gaaaccggga atattttgcg gggagaaaac
    10861 ctgaatcagt aaccaggtaa tgggagaacc gccctgatcc attgaagcca ccatgccccc
    10921 ggcttttaac gccccagcat tagccgccag ttgggtgtgc aaaccaatag tttgcgtcgc
    10981 gatccacagg gtgatgctca taatgatgac acccgagatt aaggtgcgga acagatttcc
    11041 acgatgcacc gcgaccgcca tcgccacaaa gaagccgatg gtggcaaggt cgccaaacgg
    11101 cagcacctga ttacccggca cacaaacagc aattaaaatg gtgagtggaa taaaaatcag
    11161 gcttgccgat accaccgccg tatgtcccag cagcaacgcc ggatcaaggc caatcaggaa
    11221 ctcctgaccg ccgaacttcg cctgtaaacg actacgggcc tgcttagcga tgggcgttaa
    11281 accatccatg atgggtttaa tcacccgtgg catcagcagc ataactgccg ccgtttttac
    11341 cgccagctgc aatacacctt tgacatcgta acccgcgagg atgccgataa tcagccccat
    11401 cacaaaaccc acggtgaccg gttcgccaaa agggccaaat ttgcgctgaa tatcatcggc
    11461 gctaaattta attcggttaa cgcctgggat tttctcgatg atagcatcga ccagcaccgc
    11521 aatcggcccc atatacgccg acgtaccgtg cggaatagca atgccttcca gctcaaagaa
    11581 atttcgggta tcgcgggcaa accagtcgcc gagcttataa acaaacgccg cgtgaattac
    11641 cacacccgcc atccctatca tccatgaacc ggttgccaga tgcagcaacg cgccggtgaa
    11701 ggtcatatgc cagatattcc agatatcaac atttaccacc cgcgtcatac gggtcagtaa
    11761 catcgccacg ttaaccagaa tcgcaatcgg aatcgccacc agcgcaattt gcgacgccca
    11821 ggtcatgggt gaagagcccg gccagccgac atcgaccaca tgcagattca ggtcgaaatt
    11881 ttccgccatc gctttcgccg ccgggccaat ggaatccagc attaagccaa tcacaaggcc
    11941 aataccaaca aagccaatcc cgatatgcag acctgcttta aagcaatcgc ctgccttcat
    12001 gcctaatatt ttagaaaaaa taatgatgac aatcggcagc atcaccgtag ggccgaggtc
    12061 gagaatataa cgcatgactt ctgaaaacat aggtcacccc tgtaagatag tcagaatttt
    12121 attttgtaat gcttcgatac cgacaccaga aacaaaaggc atgccgtgaa ctaacggaat
    12181 atcgccaaaa cttcgatcca ccctggcagt ggtgcatatc aaatgcacgc catccatata
    12241 ggtttctatt tcattaaccc gacactggat taattcaaca ggaatattat gactctgaca
    12301 caactcttta atttcttccg ccgccatcgt agaggtcgca accgcgcctc cgcaagcgac
    12361 aataatcttg cgtttcataa atagtccttt ttattgttac agagggattt attcgtctga
    12421 atctaaaaca tactttgtga agtattcctt taactgggtt tcaggaagag tgattagtgt
    12481 ctcgacgatc tcgggctgtt gtaacttgcc aaataaacag cgtaaaagtt tcaattgctg
    12541 ctgcggattt tccacaatta acgcaatcac caacgatacc gccacgtcgt tatcatcatc
    12601 cgcttgctga aaatgaactt tatttgttgg ccttaacaga taaatggctg acgacttagc
    12661 atgaatcgcc tcacaatgcg gtattgcaat ggcgtgctgc tcaagcatta tcccggtagg
    12721 gaattctgct tctctggcaa ttaatgcctg tggccaggta tcatgaacca cacctttcgc
    12781 gagcatctca ttaccgatat gggttaaaac ttcgctacga tcgacaaaag aaattccgct
    12841 acgaacaaac aggttagtca tataccgtcc ttattccgca cagccgtagc gataggcgcg
    12901 caaaacatca taaattttat ccataatcaa ctgatgcggt attgctgata attccccgga
    12961 ctgaatgcgt tcaaattgtt taggaagata ctgactaatc atgcccagag ggatttccat
    13021 gccttgcaga ttcaccatca tcgtttcgac gctattttta atccggctat gcggccagta
    13081 ataacgaata cgatccgaca ggctgtaacg aatatccagt aatgaatcgt taaaacccgt
    13141 acgataatat tttttccagt attgcggttc gtcaaacatc acttcttcaa ttaccgccag
    13201 gcaaccgctg cgattttctg gggcgataag ttcctgctca atttgcgcca gcgcaaatat
    13261 cgcctcacgt aaagcaaagg ttaatgccgg accgactttc aaaattgcaa aatgatcgcg
    13321 aactaattcc cgataggccg tctgagtctg gtaatcggtg gaatgcgctt cataaaccat
    13381 tttggttttt tctatccatt gcgccagcgc ctgagcttct tgcgcctgat aatgaataat
    13441 attgctatgg tcaaattcca cacccggctg caccacgatg gcaatcacgc gtgttaacgc
    13501 ctctgccaga ccgcgggcaa taaacgcttt ttgatgcgtg cgtaaagtat tggcggcatc
    13561 ttcaacctgg gtgatatgta ctgactgaat ggcgctggcc tcaccgcccg gaaccggaac
    13621 ttcggtgcca atgacatagc tcagttgctc acgctggcaa tctgtcgcca cactttccgc
    13681 agcaaagcaa agcacagcag ctcgttccgc aaccgtttct ggggctaacg gtataggatc
    13741 ccccgcgcaa gacattgacg catcaagatg aattttactg aagccggcac gaacatatgc
    13801 ctttaccagt tcgacggatt tttccatcgc cgcatccaca ttttcttgct gccagcagtt
    13861 tggtcccaga tggtcgccac cgagaataat acgctcacga gcaaatccga ctttatcggc
    13921 aatcgcaaaa acaaattcgc gaaagtctgc cggtgtcatt ccggtataac cgccaaattg
    13981 attgacctgg tttgacgttg cttcaatcag cactttacgc gtgctgttgc gatcaaatgc
    14041 cagcgccgct tcgataacca acggatgtgc agaacagact gaacatatgc caatatgttc
    14101 accagcttta tgccgggcaa ttaacgtttt catgtttttt ccttgttaaa tggcaggtgc
    14161 gttatgccct gccctcgcag ccacaatcgg caatcacttt gctcaccaca tcgcgcattg
    14221 cggatttagc cgactgcaaa taatcccggg gatcggtcgc ttcggggtgc tctgtcaggt
    14281 aattttttaa cgcctgcgag aaggcatttt ttagctctgt tgcaacgttg attttgcata
    14341 tccccagttt gatggtttgc tgaatatcct tagttgataa tcctgacgcg ccatgcagca
    14401 ccagcggtaa gttcacccac tggcgaatgt tctccagtcg agaaaaatca agcgccggtg
    14461 cgctggcata cataccgtga gccgtgccga tggcgaccgc cagggaatca attccggttg
    14521 cctcggcaaa ttcacgcgcc tgaacagggt tggtgtacaa cgcatcggct tcattgactt
    14581 gcacatcatc ttcctggccg ccaagttgcc ccagctccgc ttcgacgctg acatcaaagc
    14641 gatggcaaaa atccaccacc tctttgaccc gtgaaatatt ttgcgcaaaa ggcaaatgcg
    14701 aggcgtcaat catgactgag cgcacgccag aacgaacctt ctgagcgata tcgtcaaatt
    14761 tcgtgtgatg gtcgagatga attgccagtg gatggtgata ttgcttcgcc atcgcgctga
    14821 ccagcgccaa cagattttct gtaccggcat gagtaaatgt gccaggcgtt ccggcgatga
    14881 tgaccggcgc atgcaggttg gcagcggttt ctaccaccac ttgcatcgtt tcgagattgt
    14941 gaatattgaa tgccggaacc gcataaccgc cgcgctgtgc gttgttcagc atctgctttg
    15001 tcgataccac gtacattttc atatcctgtc gtttgttttc gatttcaaaa tataatgaaa
    15061 tgatttgttt ttaaatgtcg cgataacgat cacaaaaacg acaatatgaa aatcaatcca
    15121 ggaatgaaag gcaaaaaaaa cggcctcccg ggggaagccg taacaaagta cgcgtggttt
    15181 tatgccggat gtggcgttaa cgccttttcc ggcctacggg gtagtgctgt tcgtatgaag
    15241 cgcatcaggc gatagtaatt ttgctatcga gataaacgtc ctgcacggcg ttaatcagtt
    15301 tcacgccgtc agccatcgat ttcttgaacg ccttacgtcc aagaatcagc cccattccgc
    15361 ctgcgcgttt gttgataacc gcagtacgca ctgcatcgct gaggtcagtt tcaccgcctg
    15421 cagcaccgcc ggagtttatc aacccggccc ggcccatata gcagttagct aactgataac
    15481 gcaccaggtc aatcgggttt tcactggtca gtttgctgta gactcgatcg tcggtataac
    15541 cgtaattaat cgctttatag ccgccgttat tttccgccat tttttgtttg acgatatctg
    15601 cacctatggt tgccgccaga tggttcgcct gaccagtcag gtcggcggaa acatggtaat
    15661 caacgccatc tttcttaaag gcggagttac gcaaataggc ccacagcact gtcaccatgc
    15721 ccagctcgtg cgcacgttca aaagccgcag aaatttcttc aatctggcga cgtgactctt
    15781 ccgagccaaa atagatagtc gcaccaaccg ccaccgcgcc catattgaac gcctgctcca
    15841 cgctggcata cagcgtttga tcgtaggtat tcgggtaact tagcgtctcg ttgtgattaa
    15901 gtttgacgag gaatggaatg cgatgcgcgt aacgccgcga taccgatgcc agcacgccgt
    15961 aagttgacgc cacacagtta cagcccgctt cgatcgccag ttcaacaatg tttttcgggt
    16021 caaagtagag cgggttagcg gcaaatgaag ctccggcaga gtgctcaacg ccctggtcaa
    16081 ccggcagaat agaaagatat cctgtgccag ccagacgccc ggtgttgtac aacgtctgca
    16141 tattacgtaa caccgctggc gggcgattat tgtcaatcat cacgcggtct acgtagtcat
    16201 gtccggggag ataaagctgg tcagaaggaa tagtcataca acggtgctgt aaaaggttgt
    16261 cggcgtcttt gccaagcaac tgcgcaatat ctgtcattac tatgctcccg taaattccga
    16321 ttggatatcg gctatggatt ttcctggccc gccttttgcg ggcaatcata atcctggtcg
    16381 ttacaggcga gtttttccat cttttggatg ttttttcagc gttttctgtt ggcacgattc
    16441 atcagaaaaa atgttatcgc ggtcaagttt tcaccgcaaa ggtatttaaa aggtattatt
    16501 aaatggtatt gtcatcgcgt accttacatt acctgtcatg aaggaattaa aagatgaaaa
    16561 caacagcaaa gctgtcgttc atgatgtttg ttgaatggtt tatctggggc gcgtggtttg
    16621 tgccattgtg gttgtggtta agtaaaagcg gttttagtgc cggagaaatt ggctggtcgt
    16681 atgcctgtac cgccattgcg gcgatcctgt cgccaattct ggttggctcc atcactgatc
    16741 gctttttctc ggcgcagaaa gtgctggcgg tattgatgtt cgctggtgcg gcgctgatgt
    16801 atttcgctgc gcaacagacc acttttgccg ggttcttccc gttactgctg gcctactcgc
    16861 taacctatat gccgaccatt gcgctgacta acagtatcgc ttttgccaac gtgccggatg
    16921 tggagcgtga tttcccacgc attcgtgtga tgggcactat cggctggatt gcctccggtc
    16981 tggcatgtgg atttttgccg caaatgctgg gttatgccga tatctctccg actaacatcc
    17041 cgctgctgat taccgccgga agttctgctc tgctcggtgt gtttgcgttt ttcctgcccg
    17101 acacgccgcc aaaaagcacc ggcaaaatgg acattaaagt catgctcggc ctggatgcgc
    17161 tgatcctgct gcgcgataaa aacttcctcg tctttttctt ctgttcattc ctgtttgcga
    17221 tgccactggc gttctattac atctttgcca acggttatct gacagaagtc ggcatgaaaa
    17281 acgccaccgg ctggatgacg ctcggccagt tctctgaaat cttctttatg ctggcattgc
    17341 cgtttttcac taaacgcttt ggtatcaaaa aggtattgtt gcttggtctg gtcaccgctg
    17401 cgatccgcta tggcttcttt atttacggta gtgcggatga atatttcacc tacgcgttac
    17461 tgttcctcgg cattttgctg cacggcgtca gttacgattt ttactacgtt accgcttaca
    17521 tctatgtcga taaaaaagcc cccgtgcata tgcgtaccgc cgcgcagggg ctgatcacgc
    17581 tctgctgcca gggcttcggc agtttgctcg gctatcgtct tggcggtgtg atgatggaaa
    17641 agatgttcgc ttatcaggaa ccggtaaacg gactgacttt caactggtcc gggatgtgga
    17701 ctttcggcgc ggtgatgatt gccattatcg ccgtgctgtt catgattttt ttccgcgaat
    17761 ccgacaacga aattacggct atcaaggtcg atgatcgcga tattgcgttg acacaagggg
    17821 aagttaaatg aaaacagaac gtattctcgg tgctctttat gggcaggcgt taggggatgc
    17881 gatggggatg ccctccgagc tttggccgcg cagtcgcgtc aaagcgtact ttggctggat
    17941 tgaccgtttt ctgcctgggc caaaggagaa taacgcggcc tgttatttta accgcgccga
    18001 attcaccgac gatacctcga tggcgctgtg tctggcggat gcgttactgg aacgtgaagg
    18061 caagatcgat ccggatctga ttgggcgtaa tattctcgac tgggcgctgc gtttcgacgc
    18121 ctttaacaaa aacgtactag gtccgacctc gaagattgct ctcaacgcca ttcgcgacgg
    18181 taaacccgtt gctgaactgg aaaacaacgg cgtgaccaac ggcgcagcga tgcgcgtctc
    18241 gccattaggt tgtttgcttc cggcgcgtga tgttgattcc tttattgatg atgtggcgct
    18301 ggcctccagc ccgacacata aatccgatct ggcggttgcg ggggcggtag tcatcgcatg
    18361 ggcgatttct cgtgccattg acggagaaag ctggtcagcg attgttgatt cactgccttc
    18421 aattgcgcga catgctcaac aaaaacgcat cactaccttc agcgcctcac tggcagcacg
    18481 tctggagatt gcgctgaaaa ttgtgcgcaa tgccgacggc accgaatccg ccagcgaaca
    18541 gctttaccag gtcgttggcg caggtaccag cactattgag tccgttccgt gcgccattgc
    18601 gctggttgaa ctggcacaaa ccgacccgaa ccgttgcgcc gtcctgtgcg ctaaccttgg
    18661 cggcgacaca gacaccatcg gtgctatggc gacggcaatt tgtggcgcgt tgcatggcgt
    18721 taacgctatc gatcctgcgt taaaggcgga actggatgca gtaaatcagc ttgatttcaa
    18781 ccgctatgcc acggcgctgg cgaagtatcg ccaacaacgg gaggcgatat gagcggcgct
    18841 cgattacaca cgctgctgcc tgaattagcc acgcgtcagc cagtgatggt cgtcggcgcg
    18901 gcggtcattg atgtgatcgc cgacgcttat gccctcccct ggcgtgggtg cgatatcgaa
    18961 ctgaaacagc agagcgttaa cgttggcggc tgcgcactga atattgccgt ggcgttaaag
    19021 cgcctcggca tcgaagcggg taatgccttg ccgctcggtc agggcgtgtg ggcggagatt
    19081 attcgcaacc ggatggcaaa agagggctta atcagcctga tcgataacgc cgaaggtgat
    19141 aacggctggt gtctggcgct ggttgagccg gatggcgaac gcacttttat gtcattcagc
    19201 ggtgtggaaa atcagtggaa tcgccagtgg ctggcgcgat taaccgttgc gcctggcagc
    19261 ctgctctatt tttccggtta tcaactggcc tcgccctgcg gcgaattgtt agtggaatgg
    19321 ctggaagagc tgcaagacgt gacgccattt atcgattttg gcccacgtat tggcgatatc
    19381 ccggatgcat tactggcgcg gatcatggcc tgtcgacctt tagtgtcact caatcgtcag
    19441 gaggctgaga ttgccgccga acgttttgct ttatccgcag agataacaac acttggcaag
    19501 caatggcagg agaaatttgc tgcgacgttg atcgttcgtc tcgataaaga gggcgcatgg
    19561 tatttcagca acgacgcttc tggctgcatt ccggcatttc caacgcaagt tgtagacacc
    19621 attggggcgg gcgacagtca tgccggcggc gtacttgccg ggctggcctc tggtctgcca
    19681 ctggcggatg ccgtattact gggcaatgca gtggcgtcgt gggttgtcgg gcatcggggc
    19741 ggtgattgtg cgccaacgcg cgaggaacta ctcctcgcac acaaaaacgt atagatcgct
    19801 gcgacagtgg ctgatgctgt actcaatagg ccgttgctgt tggtcaagcg ccacttgctt
    19861 gatcaccagc accggtattt tgctatctaa ctgaatatgt gactgaaact cggcatccgg
    19921 catccgggcg ctaacgcggg aacgcgtacg ttgtgggtaa atgtgctggc tgcggaagta
    19981 gtcataaaga gaaatcccga tggcatcaac atcatgaatt aaatgtgccg gaacccacga
    20041 ttcctcaatc gataccgcct cttcatcgac atagcgaata cgcttaagca aaaagacatc
    20101 acttcctgct tcgaccgcta gctgctgcgc gacttcttcg ggacatttca cgacacgctt
    20161 gttaacccat agcgtatcgg gctttttccc gcgcaatacc acctgctgag aaaaacctcg
    20221 cgcttctttc aacgagtatt cgaagatgtt gttgatctgc gtgccgtaac cacgcgaacg
    20281 cgtcaccacg ccctcttctt ccagcgcctg catcgccttg cgcaccgtaa tgcgcgaaac
    20341 gccggttaac tgacttaaat cacgctcacc gggcaaaata ttgccatgct ccagcacccc
    20401 gctgcgcacg gcatttttta ccgtttcggc aaacttaata taaagcggcg tgttatccgc
    20461 cgctaaaata cgttcgttca gttgggcaat cagctgggta tgcgcttgtt ccatttgtct
    20521 ttttcctgcc agggagtctg gtgggagtat atcactgctt cttatttaag gcgtttcttt
    20581 aatcccatca acgaatccct gcaactcctc caccgtgcca ttaaacacat taaaatccac
    20641 cggaccatta atgccatcta cctgtccacg gtcggaatgc tgccagaagc gccaggccat
    20701 tccgtcattg tccggacgac gttgatagta gtgagccacc caccacggat attcattaaa
    20761 atagcccgcc agattggtgt gataaaaaac ggctcctgag taaataattg gcttttttcc
    20821 cgtacgtttt tcgaccattt ttagccactg acttacccgc ttgcgtaatt cttttgccga
    20881 taattttcct cgctcttcta cgtccagtac cgcagggaaa tcgccttgtg agaaatccac
    20941 cgtttgcaga aataatcgcg cctgaactga agcggatact gacggggaga aataatgata
    21001 cgccccgcgc agcaggccat tttcgcggct tagttgccag ttacgcgaaa aataggggtc
    21061 caccagcttt tcgccttccg tcgccttaat aaaagcaaac tgtaagcgga taccgttgtc
    21121 gcgcattttt gccacccgct gccagtcgat ccgctcctgc cagcgcgaaa catctattcc
    21181 gtgaatggtg taactggcag gaatgcgaat agcgaaagat ttaaccgggc gataaccgta
    21241 gaagtggatg tagtctttaa tctgaattgc tgtcgaatag aaaaaattca ccgtctgacg
    21301 cggataaatt gcaacgatgg aaattagccc aagagcgcat aataaagcgg tgaatttttt
    21361 tcgactggtg attcttagct gcattggttt catccctgaa tgtcagcgcc aaaggctgac
    21421 aataaccaaa gcagctatag tacggtgctc accaccaggc gtggaagtgg tgaaccggac
    21481 caataccgtg accaacttcc agcgtgtcgg cctgggctaa tgccgatgaa agccagcttt
    21541 ttgcctcctg tacggtgtca gtccagtttg tatggcgcgg gcgcagtgca gccaacgccg
    21601 cagagagcgt acaaccagtg ccgtgagtgt ttttggtcat aatgcgctgt gcggtaaacc
    21661 gttgttcacc ctcgcgggta aacagccagt ccgggctttg ctcatcatcc agatgaccac
    21721 ctttcattag cactgcgcca cagcccatcg ccaacagcga tcgcccttgt tccagcattt
    21781 cctgttcggt gcgcgcgtgt ggcgcgtcga gcaaggcggc tgcttcgggc aagtttggcg
    21841 ttattaatga aacctgtggc aataatcgac tgcgcagcgt agcaaccgcc gaaggtgaaa
    21901 gcagcgggtc gccgcttttt gccagcataa cggtgtcgag taccacgttt tggatctgat
    21961 aacgttgcaa ccgttctgcc accgcttcaa caatatcggt ttccgccaac ataccgattt
    22021 tagtggtgtc gattcgcaca tcgctgaaca ccgaatcgag ctgggcggcg acaaaatcag
    22081 gctcaatgcg atacaccgac tgtacaccac gggtattttg cgccaccagt gcagtaataa
    22141 ctgagcaacc ataagcgcca agtgccgaga aggttttaag atcggcctga atccccgcac
    22201 caccactcgg atcagtaccg gcaatcgtca gagcgttaat tcgtttcacg cttgcacctc
    22261 ctgcgtcaat tgccagagcg catcaaggaa atgtggaaca aaactgcctg gcccctcgct
    22321 actggcgact gcgcgttctc cggcttgttt catccagtga caggcagatg cgatattttc
    22381 cagcgtatcg cctggtagcg cacaacaggc agcgacaacc gccgataatg cacagccagt
    22441 tcctaccact ttggtcatta acggatcgcc accgtgaatg ccaatgatac gatgtccatc
    22501 ggtaacataa tccacctcgc cagtgaccac gacgattgcg ccagtttccc gtgccagtgt
    22561 ttgtgcagcg ggtatcgcgt tagctgcggc atcagtggta tccactcccc gtccaccatt
    22621 agcaacgcca gctaatgcca tgatttccga agcattacca cgtatggcgg ttggcttatg
    22681 ggacagaagt tccacacaaa aacggcggcg ataatcgagc gcaccaaccg caaccggatc
    22741 aagcgtccag ggcgtttgtg atcgtgttgc ttgctcaacg gcagcactca tcgcttgcgc
    22801 tcgtggctgc gtcagcgtgc caacgttaat caacaaggca ctggcgatag ccgcaaactg
    22861 actggcctct tcggtttcga taaccatcgc tggcgatgca ccgatcgcca gcaaggtatt
    22921 ggcagtaaaa gtttgcacca catcgttggt catgcagtgc acaagagggg aatgttggtg
    22981 aaaaaggtgt aacgtgtgcg cagattgcgc ggaactcagc aggtcgactt gcatagtttg
    23041 ctcctgccat aacgtgaaga agcaatgacc tggtggtccg tgacttccct acgctggcat
    23101 tatccagatc aggtgatacg ggtatttctc agccttcaca aagaagggca ccccgagtcg
    23161 tttggttgcg ataataaatc gcagaggagg atggtaatgt ccagcgcacg cgttgtaaac
    23221 ggggaaaaac ggcggtatta caatcgcgtt aataaattat ttgatataag aatccagcac
    23281 cttcagaacg acatccagat cttcttcacg ttttagctca tccccctggt gaacgatgtg
    23341 ttccgtcaga tggcctttaa tcacttcccg catcagaccg tttaccgcgc cacggatagc
    23401 agcaatctgt tgtaaaactg cagcgcattc atgcggttcg tcgagcattt tcttgagcgc
    23461 cacgacctgg ccctgaatct tactggcacg cgctttcagt ttctgtttat cacggattgt
    23521 atgagacatg gcaacacctg gttaacaaga atatgaaaaa tcatagcact attaatctac
    23581 tggggggtag tataaggtac tgggggggga gtagaatcag attgccgaat taatactaag
    23641 aattattatc atgaccgaat ttacaacact tcttcagcaa ggaaacgcct ggttcttcat
    23701 ccccagcgcc atcttacttg gtgcgcttca tggcctggaa ccggggcact caaaaacgat
    23761 gatggcggcg tttatcatcg ccatcaaagg caccattaaa caggcggtga tgctcggact
    23821 ggcagcaact atttcgcata ccgcagtagt ctggttaatt gcctttggcg ggatggtgat
    23881 cagcaaacgc tttactgctc aatcagcaga accgtggctc cagttgattt ccgcagtgat
    23941 cattattagc accgcgttct ggatgttctg gcgtacctgg cgcggcaaac gcaactggct
    24001 ggagaatatg cacgagcatg atcatgagca tcatcaccac gatcacgaag atcaccacga
    24061 ccatggacat catcaccatc acgaacatgg cgagtatcag gatgcccatg cacgagccca
    24121 tgccaatgac attaaacgac gctttgatgg tagagaggtc accaactggc aaattttgtt
    24181 atttggctta accggtggcc ttatcccctg cccggcagca attaccgtgc tgttgatttg
    24241 cattcagttg aaagccctga cactgggcgc aacactggtc gtcagtttca gcatcggcct
    24301 ggcgttaacg cttgtcaccg taggcgttgg cgcagcaatc agcgttcagc aggtcgcaaa
    24361 acgctggagc ggatttaaca ctctcgctaa acgcgctccc tatttttcca gtctgttgat
    24421 tggcttagtt ggtgtgtata tgggcgtaca tggcttcatg ggcataatgc gataactcaa
    24481 tcctttaggc ttgcatgtta tcttcagccc aggatgattc ttaaatcagc tatttccgct
    24541 gactctctgc tcgctaagga tgcttttagg gcatcctttc atttacactt tttacgaaat
    24601 catgggatca ctaacaaaat atcgcttgtc agttatattg tatggcagga aagatatgcg
    24661 actgatatta cagatcccca aagtggagag tttatgacca ttaaaaataa gatgttgctg
    24721 ggtgcgcttt tgctggttac cagtgccgcc tgggccgcac cagccaccgc gggttcgacc
    24781 aatacctcgg gaatttctaa gtatgagtta agcagtttca ttgctgactt taagcatttc
    24841 aaaccaggcg ataccgtccc agacatgtac cgcaccgatg agtacaacat taagcagtgg
    24901 cagttacgta acctgcccgc gcctgatgcc gggacgcact ggacctatat gggtggcgcg
    24961 tacgtgttga tcagcgacac cgacggtaaa atcattaaag cctacgacgg tgagattttt
    25021 tatcatcgct aaaaaaagcc ccctcatcat gagggggaaa tgcagacacc ttgttatttt
    25081 ttattattcg ccacttgctc gtcttgctta ttattagtcg tatttcacat tgattaatgc
    25141 ggttgcctgt agtgcaccag attttacttt atttttatca taaacatagt aactggcagt
    25201 tactggaatt gagaccgttg tatctgtaat ttttgacttc gccgcaattt cttttaaatt
    25261 tgtgtaccct ttgctgttat cagtgatatt taaaaatgtg actggcgaac tattgctttc
    25321 aagcataaat ccgactccat tatcttctgc acctttcagg atcgtgttac tgccggctaa
    25381 caaatcagaa cttgataaat acacctgcat ttttcgtgat gtttgatttt gttgacctaa
    25441 taaattttta caacgcagaa taatatcccc ttttttacta tttattgttg ctttgttacc
    25501 tgtggctggt aattgtgaaa cgggaatact ctctaaatta attaataaat cgtccggttt
    25561 gcaggttgtg gttatcgggt tgtatgacaa tgccaaatta aaggaataga cacctgcatt
    25621 ttgtgccaat gcatttctgt agctatttac acatgcatcc catgacttgc cacctaataa
    25681 acacagtaga aattgtactg cggcactccc gcgtgtcgat gatgaagcac tggataatgt
    25741 tgcaacacca ttaatcatca ctgaaccgcc gccccccgca gaaacattca cttttgcatt
    25801 actggccgca ggtgtcaccg ttaccgaata accactggtg aaactggtct gattatttga
    25861 caacgtaatg ttgtttttat tggtccattc aaaatgtaaa tttaatttaa tggtatcgtt
    25921 ataaagtcca acaataatat ccgacgggct cataggtgtg atagtatttg tactcacgca
    25981 tgacgaatca ttaaaattat gctcatatgt cgccatttgt gtttgagtaa gtgcaaaatc
    26041 cccctgaaca accaaattgt gctgaacaga taactcacta taacatgtcg catagctcac
    26101 aggcataacc atcatcatta ctataaatag cattatgcga atttccatag aaccacctga
    26161 ctattactgg caaatataat ttctactttc atcaatctct ttaccgaagg tgattgcgca
    26221 tgaaagccct tgttgcttat caattgccac attaaccgat ggcggtactt cattggtgcg
    26281 aataaataac tggcttccct ggccgacaac accaatatta tgaccatgga tatcattgac
    26341 ttcataacca aacgttaatg gttgcccatc cgttcttaac gcttttataa accagggctt
    26401 gcgctgatcg gtatcaaaat taaccagtac aaccgcgccg cgaaaagggg cggcaatttt
    26461 ccggttgcca cgtaattctg cttcgctatc gctttgcgac acatccagca tcaggtgatt
    26521 ttcccgataa ggtgtcattc cgtcgtatac caccactcca ttacggtttg ttgtgcgata
    26581 tttttgccca ttgacataag catctttaat tcctggcgca ttcattacag caaacgtttc
    26641 ggaaagacgg ttcgccagat taacgccacc cgaccaggcg acaatgcccc ctgaaacact
    26701 ggctccagcc tgtcgataag tactcgactg actataactg ccattcaccg ttgccaccgg
    26761 cgcgttccag gttaaattcg ctccagctgt cgtttcattt ccctgatgct gataactcag
    26821 gttgacccca tagttaaact gatcccggct tccaacggtt cctgataatc ccgtattatt
    26881 tgaggcaacc ccctgatcat caaacgtcgt tgagttagac atatatattt gccgacgagg
    26941 cgtcgtaacg tcatcacccc aatcaaaggg aatcgatata aaaatattaa aacgtttctc
    27001 ttcatgatga ttctcgtcat aagcatggct tgccgcgagg gtatagctta tccttcgcag
    27061 gttgttggaa taactcaact gataatcctt gctgctgccg ctacgccccc agtaatctcg
    27121 ccataacgta cttaaggaca cagaacccca gccttctggc aatgactggc tcatattggc
    27181 agaaaagcta tttttacggc caaaatcgtt ctggtaataa tcggcaatgt catagatatc
    27241 gttttcatca cggcgataat tatctttatt gtttgcccat acgtgatcat taaatgtccg
    27301 ataatcacgc gacgaataac gccaggccgc cagaccaaaa cgtgtcgacg tttggctcac
    27361 aaatttgttg taggcaattt gataactttg cccgtcaaac acatcgccgt tgtcttgtat
    27421 actatgcgac ttcgtggcat cgacggaaat ggcaccaatg cgtgtattcc agccagtccc
    27481 caaagtgaac gcgtaataat tattcgcgac catcgatccg ccatacagcg tcaataagtt
    27541 attaaaacca tactgataac ccgcctgaac aaaatcactt tgtttgctcg ccccttcaat
    27601 atggctacga cctgccgcaa aatcatattt cgacacgccg ggttgcagca tattcggcac
    27661 tgctgcataa ggcaccagat aggtggttac cgaaccgtcc gcctctttca cgctgacatc
    27721 aagatctgct ccaccaccgg ccaactgcaa atctgtaatc gcaaacgggc caggaggaac
    27781 ctctttctga taaaccacaa aaccattctg ttcaatagtt accagcgcgt tactctgagc
    27841 aatcccctgc acccgtggag taaaattttg tttcgagtta ggcaacatct gcatatcacg
    27901 aaacaaccgc acaccgctga agcgaacaga atcaaaaata tcgcttgatg tgtacatatc
    27961 acccacgcga agcgtgccga gaagttgggc aaatccacgt tccagataca gggtattgct
    28021 tttccacacc cctggattgc tatttgtttt actgaaactg gcatcagaat gcagttgcca
    28081 ccccagtaag tttaacccgc tgttaaaacg tacatatgta ctcttgctat tacccgacgc
    28141 tttatagtcg ctgtaatact gactcacata ataagaggta taaaacgcat taataccccg
    28201 ctcccagttt tccggtggaa catagccact ttccagttct tccacccacg cctgcgggac
    28261 actgaaatcg agacgaaaaa ctccgatatc ccaggtatag ctcccaccct gaacaagttg
    28321 ctcaaatgtt aaacattgct taccgctggc aaagttatcg gtattaatgc ctaaccgctt
    28381 gataatttcc cttgataaac atgtttcttg cgggttgtct ttaacaataa tctcatattt
    28441 cccgcgccat tgcttattga cataaatatc gatgtcatac tgccccggca agggttgatt
    28501 atcatcaaga cgaatatttg caacctgctg gtctttcatt ccacctatca tgaaatgggt
    28561 atcaaaggtt tcttcagctg cataagcttc aatgccgagc aataacgcta cgattgctga
    28621 tgcaagtggg gtcattctca acataggcag ctcctgcaat taaattttgt cactaatata
    28681 gttgccatgg tcatcgataa tggtcagatg ccagttattc gcattattac ttttgacatt
    28741 aatactctga ctttctaagg gggcaatcat aatagtttca taattgactt tgacattatt
    28801 agctttgaca tccgaaatcg ttacccaatt ttctgagtca ttttttatca ccaatccatt
    28861 gccactgcga tttaccagta actttttaaa tgtcgcttta tttaccggag caatacccgc
    28921 tggccggtaa aacaacttaa ttctgttttg catcgcaaac ttcagtgcat tcttaccttc
    28981 ttgttctgga ctatttggtg gaatatccag aacattcaga taaaaaatac tctctttatt
    29041 agtgggtagt ttattcggca taattttaat ttttacttgc tgcccggaat ttgcccctat
    29101 ttttgccact ggtggcgtta acatgaaagg aacctgaatt ttttctggtg gtaatgacgt
    29161 atcgccatca tcaatccacg cctgcagcag cgaagaacgg tttccctggt tcatcaactg
    29221 caccatcact tctttatttt ctgccgggta aataatgcgc gtcccgtaga taacaatacc
    29281 ggcatgtgca ggaaggacca tagaagaaat gagtaaagct aataatcctt tcatcttaat
    29341 gcctcttaaa ttaaaaggcc gccatgggga tagcggccat gtttattacc tggagaaact
    29401 tgcaatttta tttattaatc ctgcgccaca cgcataacca gagaagataa cacatcacct
    29461 gattgcactg aattatctgt cagcttcagg tattttgctt taaacggaat agcatattga
    29521 ccttccgctg ttttataact ctcaatgtta aaaggtgcag aaatatccag gacatcactg
    29581 ttatcaccta aatagagata gtaacccacg gtatcgttgc tactggctat cgccttatcg
    29641 ttgccggtta ttttgtcgtt tgtgatcaac gagagtttta caccagcaac ttctttcggg
    29701 cagctaattt ttaacatgct gctggttaca ccgactgcat catcataggt agaatattta
    29761 gctttacctt caaagacagt ggttggtaca gttggcatat taatacttac tgaatcgcca
    29821 cctgcttcga atttacacgg agattctgca atattccctt taatggttaa tgttccggta
    29881 tcaacgtctg cggcaaatac cccagcgctc ataaaaaaag aagaaaaaac agcggcagca
    29941 ataattgaac gtttcataat atgctcctta ataaaatcta tttttggttg ttaagagtct
    30001 gtatctcttg gaaacaaatt tctcacaccc aaaaagttgc cgcaatgtaa acacattgtt
    30061 ttattgattt aaagcaaatt aaccattgca atataaacac aacaaatact taaacgcatt
    30121 aaatatttat caaatctatt aattaatgta gatttctata aatggt
//
LOCUS       DAZGTJ010000061        30027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000061 DAZGTJ010000000
VERSION     DAZGTJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.061"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(355..522)
                     /locus_tag="ACHK8E_003261"
                     /pseudo
     CDS             complement(355..522)
                     /locus_tag="ACHK8E_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            672..1340
                     /locus_tag="ACHK8E_003262"
     CDS             672..1340
                     /locus_tag="ACHK8E_003262"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0345486.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1706..1819
                     /locus_tag="ACHK8E_003263"
     CDS             1706..1819
                     /locus_tag="ACHK8E_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP0345487.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            1888..2121
                     /gene="ydfK"
                     /locus_tag="ACHK8E_003264"
     CDS             1888..2121
                     /gene="ydfK"
                     /locus_tag="ACHK8E_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP0345488.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLNGKM"
     gene            2440..3030
                     /locus_tag="ACHK8E_003265"
     CDS             2440..3030
                     /locus_tag="ACHK8E_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0345489.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
     gene            complement(3128..3703)
                     /locus_tag="ACHK8E_003266"
     CDS             complement(3128..3703)
                     /locus_tag="ACHK8E_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0345490.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(3712..4614)
                     /locus_tag="ACHK8E_003267"
     CDS             complement(3712..4614)
                     /locus_tag="ACHK8E_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFP0345491.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            complement(4611..7637)
                     /locus_tag="ACHK8E_003268"
     CDS             complement(4611..7637)
                     /locus_tag="ACHK8E_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0345492.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(7702..8301)
                     /locus_tag="ACHK8E_003269"
     CDS             complement(7702..8301)
                     /locus_tag="ACHK8E_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFP0345493.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(8372..11869)
                     /locus_tag="ACHK8E_003270"
     CDS             complement(8372..11869)
                     /locus_tag="ACHK8E_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0345494.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            complement(11929..12501)
                     /locus_tag="ACHK8E_003271"
     CDS             complement(11929..12501)
                     /locus_tag="ACHK8E_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0345495.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(12498..13241)
                     /locus_tag="ACHK8E_003272"
     CDS             complement(12498..13241)
                     /locus_tag="ACHK8E_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0345496.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            complement(13247..13945)
                     /locus_tag="ACHK8E_003273"
     CDS             complement(13247..13945)
                     /locus_tag="ACHK8E_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0345497.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(13945..14274)
                     /locus_tag="ACHK8E_003274"
     CDS             complement(13945..14274)
                     /locus_tag="ACHK8E_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0345498.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(14271..16850)
                     /locus_tag="ACHK8E_003275"
     CDS             complement(14271..16850)
                     /locus_tag="ACHK8E_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0345499.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            complement(16843..17277)
                     /locus_tag="ACHK8E_003276"
     CDS             complement(16843..17277)
                     /locus_tag="ACHK8E_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0345500.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(17259..17681)
                     /locus_tag="ACHK8E_003277"
     CDS             complement(17259..17681)
                     /locus_tag="ACHK8E_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0345501.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(17697..18437)
                     /locus_tag="ACHK8E_003278"
     CDS             complement(17697..18437)
                     /locus_tag="ACHK8E_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0345502.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            complement(18445..18840)
                     /locus_tag="ACHK8E_003279"
     CDS             complement(18445..18840)
                     /locus_tag="ACHK8E_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0345503.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(18837..19415)
                     /locus_tag="ACHK8E_003280"
     CDS             complement(18837..19415)
                     /locus_tag="ACHK8E_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0345504.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(19427..19780)
                     /locus_tag="ACHK8E_003281"
     CDS             complement(19427..19780)
                     /locus_tag="ACHK8E_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0345505.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(19792..20187)
                     /locus_tag="ACHK8E_003282"
     CDS             complement(19792..20187)
                     /locus_tag="ACHK8E_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0345506.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            complement(20229..21254)
                     /locus_tag="ACHK8E_003283"
     CDS             complement(20229..21254)
                     /locus_tag="ACHK8E_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0345507.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(21310..21642)
                     /locus_tag="ACHK8E_003284"
     CDS             complement(21310..21642)
                     /locus_tag="ACHK8E_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0345508.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(21652..22971)
                     /locus_tag="ACHK8E_003285"
     CDS             complement(21652..22971)
                     /locus_tag="ACHK8E_003285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0345509.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(22952..24553)
                     /locus_tag="ACHK8E_003286"
     CDS             complement(22952..24553)
                     /locus_tag="ACHK8E_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0345510.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(24550..24756)
                     /locus_tag="ACHK8E_003287"
     CDS             complement(24550..24756)
                     /locus_tag="ACHK8E_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP0345511.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(24753..26678)
                     /locus_tag="ACHK8E_003288"
     CDS             complement(24753..26678)
                     /locus_tag="ACHK8E_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0345512.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(26653..27198)
                     /gene="nohD"
                     /locus_tag="ACHK8E_003289"
     CDS             complement(26653..27198)
                     /gene="nohD"
                     /locus_tag="ACHK8E_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP0345513.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            27588..27782
                     /gene="ylcI"
                     /locus_tag="ACHK8E_003290"
     CDS             27588..27782
                     /gene="ylcI"
                     /locus_tag="ACHK8E_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0345514.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            28143..28436
                     /gene="iss"
                     /locus_tag="ACHK8E_003291"
     CDS             28143..28436
                     /gene="iss"
                     /locus_tag="ACHK8E_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP0345515.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(28527..28709)
                     /gene="rzoD"
                     /locus_tag="ACHK8E_003292"
     CDS             complement(28527..28709)
                     /gene="rzoD"
                     /locus_tag="ACHK8E_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP0345516.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(28926..29423)
                     /gene="rrrD"
                     /locus_tag="ACHK8E_003293"
     CDS             complement(28926..29423)
                     /gene="rrrD"
                     /locus_tag="ACHK8E_003293"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0345517.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(29423..29638)
                     /gene="essD"
                     /locus_tag="ACHK8E_003294"
     CDS             complement(29423..29638)
                     /gene="essD"
                     /locus_tag="ACHK8E_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0345518.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         6730 a         8737 c         7242 g         7318 t
ORIGIN      
        1 agttacattt ttcaacgctt actatacttt ttattaacat aaactcacta caacgcacct
       61 gaaacctctt gctatatata tgtcaaccgt ttgaatttaa aataaaaaga gtatcatttt
      121 tacttgcatt tcttatcaag tcacattcaa caacagtaaa aaaacattat tagaaccatt
      181 caattaacaa aaaaaccaac atccagcttg cttaattttt ctttattaaa cgatattgaa
      241 aatcaattga taaaatacat ctaaacaacc ttttggggcg caaaagcata acatcaaaca
      301 aataacacac cgaaaaaact cacaattaat aacctatgat atacatactg tttattatgg
      361 ttgaataagc cactcgatat ctggtgctac ggaagtgtcc acacggttta gcagcacccg
      421 atactttttt caggcttcca gcaacgatct ttcttcctcc gttgcgattt ccagatctac
      481 agcatcctgc agtggcgcaa tatactcact gaattcctgg atgtagaact gtgtggtgac
      541 ggtcttccag ctattcggct cctgctgtat tgaagcatac caggctattt caatatcgct
      601 atgctgcggc agcatttaac cccttgtaat tcattgccat aattgattta attcacaaat
      661 aaaactataa catggtgaaa ttaatgaaaa aaaacacaga tgatggggct aaaatttaca
      721 caccacttac cctaaagctt tatgactggt gggttttggg agtatcaaat cggcttgcat
      781 ggggatgtcc tacaaaggaa caccttcttc cacactttct ggaacattta ggtaacaacc
      841 atctggatat tggtgttgga actgggtttt accttactca cgtacctgag agtagtctga
      901 tatctttaat ggatttgaac gaagctagcc tgaacgcggc atctacaagg gctggagaat
      961 caaaaattaa acataaaatt aaccatgatg tttttgaacc ttatcccgcg gcgttacatg
     1021 gtcaatttga ttccatttcc atgttttacc ttcttcactg cctgcctgga aatatatcta
     1081 caaaaagctg tgtaatacgc aatgcggcgc aggccttaac tgacgatgga actctatacg
     1141 gagccacaat tcttggcgat ggaattgtgc acaatagctt cggtcaaaaa ctgatgcgca
     1201 tttacaatca gaaaggcatc ttttcaaaca caaaagattc cgaagaaggc ttaacacata
     1261 tactctcaga gcatttcgag aatgttaaaa ccaaggttca aggtactgta gtaatgtttt
     1321 ccgcttcagg gaaaaaatag catccaaccg cagcacgttc ttgcataagg cgtgtctgcg
     1381 gtttttcaac tattcagata catcactccc atcacattca ttcctccgca tcataggcat
     1441 ataggctata tcaccttgat atttttcttc ttcagataaa aactgttatc tatgtatact
     1501 tttaaaccca atccgtgtag agtctctgca taagatagtt tgcagttgac acttcagctt
     1561 gcgccataaa ccgcctgatt tttgccgcca cctgttagca ttcctgtata cctgaaacga
     1621 caatgtttat ctacgaactt taagaacacc caggataaaa attgtcaact atatcatata
     1681 taacacatta ctaattcgag gctatatgaa cagcatactg ataatcactt cgctccttat
     1741 catattcagc atttttagtc atgctctaat aaaattaggg attggcatat ccaataaccc
     1801 agacaaaacc gatgtataag tcaacatatc ctgaatcaga catacaatat cgcaatgaaa
     1861 atcaataata ttttaaggaa tatcttcatg aaatcaaaag acaccctaaa gtggttccct
     1921 gcgcagcttc ctgaagtaag aattatccta ggggatgctg tagtggaagt agcaaaacag
     1981 ggaagaccta tcaataccag aacattgctt gattacattg aaggaaacat aaagaaaaag
     2041 tcatggctgg ataacaaaga attattacaa acagcgatat cagttcttaa agacaacaaa
     2101 aatttaaatg gtaaaatgta atataataaa cttacttttt tatcattttt ccactttaac
     2161 aacattttgc tccacttttc cacgaccaaa caacttgaaa tctggttaaa ataacacgca
     2221 acactattct tcttccttga gtccgcccgg aactcgaaaa acaaaccgag ttaaggccat
     2281 ttttcacaaa atcgattttg ggtctcacca aaattacggg gttgcatacg cattcgttta
     2341 ttttcgaacg tgtacataca aatatgtaca aaaataatca aaattatttt ctgagatgca
     2401 ttatgatatg tacaccaatt tcgtatagaa gatctcacta tgtctcgaat ttatgcttac
     2461 tgtcgggtat caactctgga aaagacaacc aaaaatcaac gtcgggaaat cgaaagtgca
     2521 ggttttaaca tcaaatctca gcaaataatt gaagaacaaa ttagcggctc agcagcaacc
     2581 agtgagcgtc ctggttttaa ccagttgctt gatcgactga aaagaggtga tgcattgatt
     2641 gtcacaaaac tggatcgcct cggttgtaat ccaatggata tcagtaaaac ggtggaacaa
     2701 ctgaccaaag caggtatcaa agtgcattgc ttagcattgg ggggcgttga cctgaccagt
     2761 ccacctggaa aaatgatgat gcaagtaatt tcagcagtcg ctgaatttga gcgagacctt
     2821 ttacttgagc gtactcattc agggatatta agagcacggg gcgcaggaaa gcgatttggt
     2881 cgcccacctg tgttaaatga tgaacaaata cagagggtac tagagcaaat taagtcaggt
     2941 ataaatataa gtgacattgc ccaagaattc aaaacctcgc ggcaaaccat tttaagagct
     3001 aaagcaaaaa atcagacacc tgatatataa aaataatctc ggtgtgagat tccttacgtt
     3061 ttccaagccc cccttcctag ccttaaatgg aaagatactt ctaattatag aatttatata
     3121 tcttacccta cggtagggct ggccattcaa tatccggtgc agttgatgta tcaacacggt
     3181 tcagcaacac ccgatacttt ttccaggctt ccagcaacaa ggtttctttc tccgttgcga
     3241 tctccagatc cacagcatcc tgaagcggcg ctatatgctc actggctacc tgcatcaggc
     3301 tgttttttgt ttcttccgcc tcccggatcc ggaacagttt ttctgcttcc gtatccttca
     3361 cccaggctgt gccgttccac ttctgaaact ccccttccgg ggataaccag gtgacatttt
     3421 ccggtaatga gccgagttca gaaataaata acgcgtcgcc ggaagccacg tcataaaccg
     3481 ttttaccccg atgatcttca acgagatgcc acgatgactc atcactgttg aaaacagcca
     3541 cgaaaccagc cggaatatct ggcggtgcaa tatcggtact gtttgctggc agacctgtat
     3601 gaggcggaat gtatgcgtca ccttcaccaa taaattcatt agttccggcc agcagattat
     3661 aaattgttat ggtccgtggt tgttcactca ttctgaaatc catgttcacc tctacttaat
     3721 atcagagaca gaatattgtt tattgagcgt atgggtgtga gcaccaataa cgatgctact
     3781 tccgtggcta tgtgcgccag agatgacatt acctacagga ttttctggat taatccggtg
     3841 tatatgcgaa ccagggcgaa accggttatt agcctgatgt tgtatcagta catcatcagc
     3901 ctgatgttca gggttaatac agacctgccc ggaacgtgag caatacgtcc gataatcgcc
     3961 gcatccaata tatttagtat ccgcataacg gcaaacagaa ttacccggac agtatgcata
     4021 tgtgccaaat actgccgact ggcgggattg tgcgtattcc cagttaattg attctgtttt
     4081 tttattaaag ttatcccatg cctgcttcat ctgccgggca acgctttcaa acggcacctg
     4141 accacatccg gctcccattg cagggaatac caccgtcttt attttcctgt ctgtcgttgc
     4201 gtttttattg tgctgaaaga tagcaagtag agctgcccaa gttgcgttat atacagcgtc
     4261 tgttccgtca attgtcaacg gaacacgcat tgttggcgca tgtaccagcc aggggtgata
     4321 attatgcccc gtttcaataa caaatgcaga acctacaggc tgctcgccga gatattcacg
     4381 aagaatatga ttctgaacgc gggcctgtag ctgagaaccg aagaatgtgg taatggcggc
     4441 atcaacaccg ccatccatca ggccgaaact atttgccgca cttaccatgc agtcaaattc
     4501 tctgattgtt tcaaatggct ttccgacaat attcacatta tctgcatttg caaatacccg
     4561 cttaaatgct tcagccattt cggttactgg tgcagaaaga atgagcgtaa tcatgcaagc
     4621 ctcacaatat agttaaatgc gatgtttttg acggtgtttt ccgcgttacc agcagcgtta
     4681 acggtgatgg tgtgtccatg tgagccaatc gcaaccgagt gcgtatgagc accaataccg
     4741 acagtatgtg cgtgagcacc tgcagatgca gctgtgccgc tgacactatg agtgtgcgct
     4801 cctgcagcac ttgtattcac actggccgca gacactacct tatgtactga cccattttgt
     4861 gaccactggc tgactgctga tgcgcctgtg acactatgag tatggtttcc ggcacttgcg
     4921 gctgtaccgc tcacactgtg cgtatgcgcc ccgatgttat tcgtggattt agtgccgtaa
     4981 tcaaacgacg atgtggtttt cgtccccaaa tccgtactgg atgcgctggc gctgtgggtg
     5041 tgcgatttaa tgccgtcctg ttcctgagac aatacggccc gaccactggc aggtttgccc
     5101 ttaatcgtcc agccacgcat atcagggatc acgcctgacg gataagcggc tgcaagtttc
     5161 gggtatgcag atttgtcaaa agtctgcccc tgcatcagga cataaccaga cggaacggta
     5221 tctgatggcc acgggattgg tgcaccgact ggataaaact ctgcaggagg atgagccgag
     5281 gtgtaaagct gcgcccacgg cgaccagttt gcgtcggtcg tatcccgtcg tgaacgaata
     5341 aatgccggag catgagcacc gcttgtacca ctccagccga tgagtaactc accttcgcca
     5401 acggctgtca tccctttcag gtgaatgata tttccatacg ttgttggata tccgttgtta
     5461 tacacctcgt ataactcaag acctgctgcc ccctgcgtat tgtctgtcag cgcggctacc
     5521 cgaccttttg aagccagatt aactgatgat actgctgttc cacctgacgg tagcgccccg
     5581 atctctgatg ccgttggctt atttctggag ttatagtccc ttcgccagcc aggtgaataa
     5641 tctgttccgt gattaatata ggtaaactgg gcgttggttg ttccgccacc gctggatgtg
     5701 gtcggagtgg taatgcggat cgtcatcgct gactttatcc ccattacttc aatgacagct
     5761 ccggcgagat gaatattacc gcagccagta tcagtaatga ttttattatt gccataagac
     5821 caggaaccct tgcacatcca gtatggatgg ttaaatgctc cctgagaatc cagccactcg
     5881 ataaactgtg cagtcgtcca gtttcctgtt gttgtgctta ctgacccacc gaaggcacgg
     5941 caggcaccaa tatttttcgt aaaggtgtct ttgccaggga tatccgcacc gttctgatct
     6001 ttctgcagac gtttctcagc attgtcattg gctgctttta ctgcctttgg cgttgccgcc
     6061 agcgtttcag acgtgctgtt ggtcgcgctg cttagctgga ttatcccttt ctgtgctgtc
     6121 gttgcatcct gtgcggtgta tttcccgcta gccaggtcat acgcggcctt aacggctttt
     6181 ggtgttgccg ccagtgactc ggaagtgctg ttagtcgcac tactgagctg tactatcccc
     6241 tttttcgtcg tgctcgcatc ctcaagcgcc acggcggatg caatatcctc tgcccgtttt
     6301 gccgctgtct cagcgcgcgt tgctgcagat tccgccgtac ttttgctctg agctgctgcc
     6361 ttcgcactac cagcagcctc tgtcgccttc gtggatgccg tcgtggcgct gcccttcgct
     6421 gctgacgcct gtctggtcgc ctcatctttt gaagcagacg ccgatgatgc cgatgacgcc
     6481 gccgaactgg cggacgatgc cgctgccgtt tttgaggatt ctgcacgggt ttccgacgct
     6541 ttcgcgttcg tttcggatgt cttcgctgcg gaagctgacc tcgctgctgc cgtggcctgc
     6601 tcagtggctt cgccagcctt cgttgtggct gttgaagcgg atgatgcggc gctttctgcc
     6661 gattttccgg cggcggtggc actggctgag gcctgcccgg cacttgttga cgcggcactg
     6721 gcagacgacg cagccgctgt ttttgagcct gccgcagctg aggcgctctg tcccgctgcc
     6781 gtctcagaag acctggcgtt cgtctcggac gtttttgccg ccttcgcgga atttcctgcc
     6841 gccgttgccg aggaagctgc gctactggcg ctcaaggatg cgttcgtttc tgatgatttc
     6901 gctgcctctt ttgaggccgc cgcatcccgg gctgaggtgg cagcttctga cgctttcgtg
     6961 gtcgcggtgg atgcagaagt ggcggctgat tgttctgacg ctgcggcatt cgtttctgac
     7021 gttttcgccg caccggcact ggtagctgcc acgctttttg aggactctgc agcggcagca
     7081 ctttttgagg cttcagtggc ctttgttgat gccgttcctg cgctggaaga cgctgactga
     7141 gccgacgaag cggcctgtcc ggctgacgtg ctggctgcac gtgctgaatc tgcagcatca
     7201 gtcgcatggg ttgccgcctc acgggctgat gtgctggcat cgctggctga cttcttcgcg
     7261 gctgccgtgt tctgtgccac cgcggacgcg ttacgcgcca cctcttccac catcagttca
     7321 aaacggcgca gtgcctcagg acgggcatca tcctccgtca tggcaccgag aaaatcattc
     7381 agcgtaccgg gtcgggaatc ttcatacacg gtgatggtcc cggcatgtga cggcgggaat
     7441 ccctccacca acagaataac gctgtactgc ccgtactcaa cgtccatgct gtaacgcccg
     7501 gcttcatccg gattttctga ggccagcgtg ttcaccacca ccgtggtgct gttacgtttt
     7561 gctttcagct ggattgtgca gttctgtacc ggttttcctg tgccgtcttt cagtacacct
     7621 gaaatcttta ctgccatatt caccccacaa aaaagcccgc ctgaaccggc gggctgtcat
     7681 aacactgtgt tacctggcta atcagaattt ataaccgaca cccacgatga aaccgtcagt
     7741 gcgccagtca ccactgccgg agccttcata agcaatatca atggccacgg attcggtcgg
     7801 gttaaactgc acgccagccc cccacgccag agacgtgttg ctgtggcgac cgtcatcact
     7861 tccggtcagc acatcgtgct ttttcccctt gttgtcagtt acgcgaagat aatccccgga
     7921 gaaagtcgac acacggctgt aagctacacc cgccatcgca tacgcgctga accattcatt
     7981 cacgcgcaca gacggccccg ccattacgct gaaccagcgg ttacgaacgg aatcttcatg
     8041 ccagcgggta tcgctgtaac gggtcagctg gcgattcttg tctcctgcat agctgaatga
     8101 cgtcaccagc cccagtgtgt ccgtaaactc ataacggtat ttcacgttaa tcccgttcag
     8161 atcatcgctg ccggggacgt tcgtcgaggc atgaagatac cccgcgctca gcgtggactg
     8221 atgttcagac gcccatgcag gcgcaccgga tacggccaga caaatggctg cggacaaaat
     8281 tgctgcacaa actttacgca taattacctc tcgcttttct gcaataaaaa aggcgtcatt
     8341 cctgacgccc tttattgggg ttataaatat ttcaacgaat actgatgccg gaagcggctt
     8401 ttttggtcac aatcaccgta cagtcggtga tattgcctgc cccctgattg cctttctgga
     8461 aaatcttaaa ctccagagtg acgcttcccc tgccactcgg catatcaata actgcactgt
     8521 aactaccggg aatggcccct ttagtttctc tggatgcgat taatacgccg tttttgcgaa
     8581 cttcaaaacc ataacccgtg tatcgcgtgc ctcctgggtt atttccgctc cccggatcgt
     8641 catacgctat accgttaaaa ataatgggcg gaataataat ctggcggtca aagttatgat
     8701 catcgctgat ggtgactgta accgtaccgt ttggtgtttc cgtgttaccc cacgtaccga
     8761 cttttttcgg gaaggctttt gatacagctt taacgaaatc tcctctgacc tgggtcgcct
     8821 ccagcatgcc cttaatcgta cagttctggt taatcgtgac attgttgagc gttcctgagt
     8881 tcgcattcac actgccactg atatccgcat ttttcgccgt cagtctcccg tctgatgtca
     8941 gggaaaatgc cggaggatta ccgccgctgg taatggtggg ggccgtcagg cgtttcagga
     9001 acacttcatt catgaatatc tgatcgccct gaccaacaaa catcggcttt gtgttgccat
     9061 tcgcaggatt aatcatcgca atcctgtctg ctgccagcag cacctgactc tgcatgccgt
     9121 caggggtgtt ctcaataccg gcaccgatac ccgcaatata aaggcgtccg tcctgcatct
     9181 gctgcagctt cactgcccac atgctgttca ggttattatt tgtatcaacc tgaaccttct
     9241 gtatctgctg gatcgctgca ctctggtctt ccagtttctt attgacggtc tgtgttattt
     9301 cattgctgac atccgttatg gacgtcctga tttcagtcag gtcaggcgca agctgaccgt
     9361 tatcaatctg cgtccacagc tcctgagcca gatgggtttt ccctatctcg cctttgaaaa
     9421 aatccagata gcctgatgca tcatcactcg gctgaccgac agcctccacg aatgccgatt
     9481 tgccaacggt gttcacactg cgaacgtaaa aataataatc atggcccggt ttgatattga
     9541 tactggcggc tatccagtac agtgccgtac caagataacg cgcgctggtt tcaacctgcc
     9601 tgatatccgc aatccgtttt tccgagaacc agaactcaaa ctgtaccgtc gggtcataaa
     9661 ccgcaagatg cggcgtggcg gttatctgaa aatagcccgg tgtcagctca atccgagacg
     9721 gcgctgccgg tgcggcaatc cggaacgata ccgatgccgg atcgccctgc tgtccccacg
     9781 catttaccgc ccggactgtc agcctgtagt tccccaacgc cagttgtgtg aagcggtatg
     9841 tggtttccgt cgtccgggcc gtgctgacca gccgctcact gccgtcgtcc gctgttacgg
     9901 tcagacggag caggaagctc acgcccttca ccaccttcgg tgtgtcccag cgcgccagca
     9961 cctgatattc cccgctgtct gcggtgactt cggcagtcag gtgctgcacc gctggcggcg
    10021 tgacaccatt caccgtgccg ctctggtcgc cgtcaaagtg cgccccgtta tccacgatgg
    10081 cctctttttc cggcacatgc tgcacggcgg tgatggcata cgtgccgtcg tcgttctcac
    10141 ggatactcac gcagcggaac agtcgctggc gcagcgtcgg cagcttcagc ccccacacgc
    10201 tgtatccggc aacgccgtca ggaacacggc tcacttttac cttcacgccg tcggtgacgg
    10261 actggacctc cacgctgacc ggattgccac ttccgtcaac caggcttatc agcgtggtac
    10321 cggaggatgg cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg ctgttcaccg
    10381 ccagcacgcg cccgccggtg ctgatacccg catagtcatc atcgcagatt tcaatgacat
    10441 cccccggtac atggcgaagc ccttccgcac ccacgctgaa gtccacggtc tgcgtttcca
    10501 gcagttccgt tttaatcagc cacagcccgg cgcggtgcgc ctgcccccga ctggtacagc
    10561 caaaggcatc catcttcgtg acgttacgac cgtaacgggc aatggcctgc gtgtcctcca
    10621 caagctctgt cgccgtctcc cagccgttat ccgggtcaat ccagttcacc tcaacggcat
    10681 tatggcggtc cttcagggcg ctgaagctgt agcggaacgg cgcgccatca tccggcatca
    10741 ccacattact gcggttatag gtccacacct tatctgatgg tcggtcctgc acgaacgtca
    10801 gcgtctgccc gttccatatc ggcatacagc gcatcgccga gcagaaatca ctgagcacat
    10861 cccacgcctt gcgctgtgtg gtcagccagg cattacaggt gatgcgcggc tccgtgccgc
    10921 caaagccgtc cggcaccgac tggtcgcaat tctggccgat gacatacagc gcccatttat
    10981 ccacatccgc cgcaccaaga cgtttcccca tgccgtagcg cggatgggtc agcatatccc
    11041 acagacacca ggccatgttg ttgctgtatg ccggtttaaa cgttccgtcc cagataccgc
    11101 tgtattgccg cgtctgcggg ttatagttcg acggcacctg cagaatacgc ccgcgcagat
    11161 gataattacg gctcacctgc tggctgccga actgctccga gtccacctgc acaccgacca
    11221 gtgccgtgtt cgggtagcac tgtttcacat cgatgatttc ggtgtatgac gaccagagcg
    11281 ttttgttctg cagctggtct gtggtgctgt ccggcgtcat cctgcgcatc cgtatactga
    11341 acgggcgcgg cggcaggtta cccaccacca ccgaggccag atactgcgag gtggttttgc
    11401 ccttaatggt gatgtctttt tccgtcaccc agccaccatt acgctgtatc tgaaccagca
    11461 ggcggacttc cgacggattc ctgtccccct ttgaggtggt ttccaccagt gcctgcacgc
    11521 cgaaggtaac acgcagtcgg tcaatgtttg ccgacgtgat ggtccaggtg atcggcgtgt
    11581 cgtatttcac ttccgtaccc agcaccgtct cggagccgga ggattcaaat ccctccggcg
    11641 gtgtctgctc ctgctcaccg gcccggaaca ccaccgtgac gccggagatg ttagtattcc
    11701 cctcactgtc cagcactggc gtactgttca gcagcacgct ttttaatcca tccaccggac
    11761 cttcaatcgg cccttcgctg atggcatcga tcacactcag caactgcgtg gacttcaggt
    11821 tgtccttcgc ttcgcgcgga gtatgcccct tactgctgcc tttacccatt cctcacgctc
    11881 cataaacgac aaaaccgccc ggaggcggtt tcacataaac gtttttcatc agcgaccaat
    11941 caccacaacc tgaccaccat ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg
    12001 agaccccacg cgcatttccc cgtacagaac aggcagaaca ttgccctggg caaccatgtt
    12061 atccagtgag gagaaatagg tgttctgctt accgttatcc gttgtctgtg tacggggagt
    12121 tctggctttc ggtgccagca tctgcgccac accaccgagc accatactgg caccgagaga
    12181 aaacaggatg ccggtcatac caccggcccc aatggctgcc ccccatgctg caagggtggc
    12241 tccggcagta aagaatgatc cggcaatggc ggcagccccc agaacaatct ggaatacgcc
    12301 ccctgacttg gccccggcga ctctgggaac aatatgaatc acagcgccgt caggcagagt
    12361 ctcatgtaac tgcgccgtta acccggacgt gctgacgtcc cgcccggcaa tccgtacctg
    12421 ataccagccg tcgctcagtt tctgacgaaa cgccgggagc tgtgtggcca gtgcgcggat
    12481 ggcttcagcc cccgttttca cacgaaggtc gatgcggcgg ccaaatcgtt gcaaatcccc
    12541 gtaaaggcag atgcgtgcca ttcccggtga cgccagaggg agtgtgtgcg tcgctgccat
    12601 ttgtcggtgt acctctctcg tttgctcagt tgttcaggaa tatggtgcag cagctcgctg
    12661 tcaccacagt aaatggcggc atgattcggc accgatgaac caaaacagca cagcagcaca
    12721 tcgcccggtt gtgctgatga caacggcacc tgatacagcc ctgtggcctc cagattatcc
    12781 agatagagat tctgaccgtg acgccaccag tcatcctcgc gatgaaaatc cggcatctca
    12841 atccccgcca gatggtaagc atcccggaac agcgtgtaac agtccgtcac cccgtgctca
    12901 aagcgccgcc cggaaagatg tggcacacag cggaacttgt gaatcgcccc ccggcaaacc
    12961 agccaccacg gcaaatcact ctgcacctgc agccgccggt cggtctcact cagccagggc
    13021 agaccaccgg ggtggctgtg gaccagcgcc acaatctcac cctgcatctc tgcccgcagc
    13081 cagtcctccg gcgacatccg gaaatacgcc tccggctcac cggagatatt cacgcaggga
    13141 aaatatcttt ccccctccgg tgttctcacc acgaagccgc acgactccgc tggcgcacat
    13201 cgccgggcgt gcgccagaat cgctgattct gtctctgtca tgggattcac tgcgaaagtt
    13261 tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcgaaactta cagccgctca
    13321 ggcatttgct gcatttatcc ttcgtgatat cggacgtcgg ctgatcatat tcatccgcga
    13381 ccgccggacc gtgataaccg cactcatcac cgcgataggt ccaggtgcag gtgttggcca
    13441 gcatgatacg tcccggaaaa acggcaccgt ccgtttccgt cggcgtggac agtacaaaag
    13501 aggcactgac cgcgctcagt tcgctgcact gctcgatgcg ccagcggctg atcacctcct
    13561 gctccggatc ggcgtcactg tttccgttga cgaagttcac cgcatccaga aaacgggcgt
    13621 aaaccttacg ccggaccacc gttccgccga ccagactctg catatcttcc gccatcccgg
    13681 tgaccatacc gtacaggtta gaaaccgtca gcgtggggcg cgtactggtg cctttgccat
    13741 tcagttcaaa accgctcccc tgaatgggat acggctgata ctgtcgcccc tgccaggtga
    13801 ccggctcacc tttttcgttc tgctcattac agaaaaaata acgttctcca ccgacctctg
    13861 tcaggtcgat ttcccagagc accacgctgg ccgactgctc cgcacgggtg cattcattca
    13921 gtgtttcctg ccggatatcc tgcatcagtt caccacctgt tcaaactctg cgctgaactc
    13981 aacacgcaac atactgaccc gcgacgacca ttttgcgcag gtcaccttta tctgccgcca
    14041 ctcataaggc ggcgtccaca gaaaggattt ccagcccccg tgctcttcca gaaacgactc
    14101 cagtaccgtg gcctcctcac gggggacaga aagcgtcacg ctgtacgttt tcaggttggc
    14161 attcagcccg gcaggcgctc gctgagaata gccatcacca aagcgcacct ttcttacaga
    14221 agggaccgaa gccacatcca taccgggttt cactttccag cggaaggtct tcatcgtcca
    14281 cctccggaga acaggccacc atcacgcatc tgtgtctgaa tttcatcacg ggcacccttg
    14341 cgggccatgt catacaccgc cttcagagca gccggaccta tctgaccgtt cgtgccgtcg
    14401 ttgttaatca ccacatggtt attctgctca aacgtcccgg acgcctgcga ccggctgtct
    14461 gccatgctgc ccggtgtacc gacataaccg ccggtggcat agccgcgcat cagccggtaa
    14521 agattcccca cgccaatccg gctggttgcc tccttcgtga agacaaattc accacggtga
    14581 acaatccccg ctggctcata tttgccgccg gttcccgtaa atcctccggt tgcaaaatgg
    14641 aatttcgccg cagcggcctg aatggctgta ccgcctgacg cggatgcgcc gccaccaaca
    14701 gccccgccaa tggcgctgcc gatactcccg acaatcccca ccattgcctg cttaagcaga
    14761 atttctgtca tcatggacag cacggaacgg gtgaagctgc gccagttctg ctcactgccg
    14821 gtcagcatcg ccgccatatt ctgtgcaata ccatcaaagg tctgcgtggc tgcacttttt
    14881 acctgcgaca tactgtccgt ggcgctctct tcccactcac tccagccgga cttcaggcct
    14941 gccatccagc tcccgcgaag ctggtcttca gccgcccagg tctttttctg ctctgacatg
    15001 acgttattca gcgccagcgg attatcgcca tactgttcct tcaggcgctg ttccgtggct
    15061 tcccgttctg cctgccggtc agtcagcccc cggcttttcg catcaatggc ggcccgtttt
    15121 gcccgttgct gctgtgcgaa tttatccgcc tgctgcgcca gcgcgttcag gcgctcctga
    15181 tacgtaacct tgtcgccaag tgcagccagc tggcgtttgt actccagcgt ctcatcttta
    15241 tgcgccagca gggatttctc ctgtgcagac agctggcgac gttgcgccgc ctcctccagt
    15301 accgcgaact ggctctccgc cttccacaaa tcccggcgct gctggctgat tttctcattt
    15361 gctccggcat gcttctccag cgtccggagt tcagcctgaa gcgtcagcag ggcagcatga
    15421 gcactgtctt cctgacgatc gcccgcagac accttcacgc cggactgttt cggctttttc
    15481 agcgtcgctt cataatcctt tttcgccgcc gccatcagcg tgttgtaatc cgcctgcagg
    15541 attttcccgt ctttcagtgc cttgttcagt tcttcctgac gggcggtata tttctccagc
    15601 ggcgtctgca gccgttcgta agccttctgc gcctcttcgg tatatttcag ccgtgatgcc
    15661 tcagactcgg cccagtcctt tgctgccatc tctctggcct tttcaagatc ggcctgcaac
    15721 gtggcggctg aaagcccaag ttgcgcattc gctctgtcct cccatgctcc ccggagattg
    15781 gcaagaaatg ctgaggtttt accgcgccgg tggctccggc tctgatacca ctgccatttt
    15841 ttgtccgcct catcaaaagc cttttctgct ttctccagca ttccctgggc agtgtccggg
    15901 cgaccaatat ccagcaccga atcccacatg gatttgaatg cccgtgctgt cctgtctgcc
    15961 caggtctcca gcgtgcccat gttctctttc aggcggcggg tctggtcatc aaaccctttc
    16021 gttgcggcct cgttcgccgc ctgcaatgcc ccggcttcat ctccggaacg ctgcaactga
    16081 gcaacatacg caatctgctc cgccgtcacg ttatggaact gacgtgccat cgctgtcagt
    16141 cccgacgtcg ggtctgtggt cagcttcccg aaggcttcag cgaccttgtc cacctccacg
    16201 ccggatgcag aggagaaacg cgccacactc tggctgatcg cctcaaactg ctcaccacca
    16261 cgcacaccgg cattcaccag cgccgtcagt gactcgctgg tctggttaaa cgtcagccct
    16321 gccgcctgcc cggctctgga caggaccagc atacgatctg ccgtcagtcc cgcctgattg
    16381 ccggaaagga ccagcgtttt gttgaaatcg gacagggttg agttgccctg ataccaggca
    16441 tacgccagcg caccggtcgc caccgccagc gaggtggccc ccaccatcgg cagggtgatc
    16501 gcaccggcaa gccccctgaa catggggatc atcccgccga aggagtcctt aacctgaccc
    16561 ccctgttgca gcaggatcag ccacgggctt tgcccgcctg caagctgcgt ggccacgtcg
    16621 gtgaactgtg caggcagcat acgcatggcg gctttatact gcccgacgga aatccccgct
    16681 ttctgtgcag ccagcgcctg tcggctcagc gactgttcaa cgactgccgc tgtttttttc
    16741 gcatcagttt ccgtaccgga aaaatgacgc ctgactctgg ccatctgctc gtcaaatctg
    16801 gccgcatcca gacttaaatc aacgaccaga tcgcctaccg gttcagccat accggactcc
    16861 tcctgcgatc ccttctgata ctgtcatcag cattacgtca tcctccgtca tgtccgccac
    16921 atccggggaa gcggggataa cttcattccc gtccgggcca aagcggacgc ctccggcaag
    16981 ccctgccgct ttctgcatca gcacatcatc ttcaggctct tcgtcagcct cacgccggtt
    17041 cagcagactg aaatccagcg gatgcatatc cggatcgctg aaaaacaggc tgagcacggt
    17101 gtacgtcagc ccggaaaagt gcatatccag cagaacatca tgaaaataat gggtactgta
    17161 aaagcggtgc cagtcggcat actccgtgga tgacatcccg gcaagcatgg cacgccagtc
    17221 gggtcgcccc atctctcgcg ccagtttcag ggcaaaactc agctcaccgt cgaacacttt
    17281 cccgcagaaa caggctctgc gggcccggcg tcctctgtct gttcaggggc attattcaca
    17341 acaaactcat acataccaga cagccggtac accacgtttt cagcatgaga aattgcctcc
    17401 gtgggccagg tggtaagcac ttcctgctca atctgtttaa cggcttcatt catggacggc
    17461 atctgcgtct tctgcggatg gttatgccac agggacatcg ccaccagaaa cgcgccggtt
    17521 ctgatggcgt cttccacagt aaacttccgg ttgctgtctg actccgcctg ttctgcctgc
    17581 cgtttcatca gggcgagatg ctcaattcgc tgcagggctg acagttcaga aagcgtgacg
    17641 gtcacaccgt tatgttcaaa tgattcggtt ttcaggaaca tcgctgactc cccggattaa
    17701 ctggcggtga cggtgatttc tgcaaccgca gcaagttcac cattaccgga tacgaccgga
    17761 atgttgacct tgcctgcagc aacacctttc acggtgatgg tcataccact gaccgacacg
    17821 gtggcttttg ttttatccgc agacaccgca cggaagctct tgtcggttgc gccttccggc
    17881 tggaatgcca cggtcagcgt ggtgctctgc cctttcacca ccgaggtgct ggcaggcgtc
    17941 acagtcatgc cggttgtcgc cgtcaccgtg ctgcgatctt ctgccatcga cggacggccc
    18001 acgttggtga ctttcaccgt gcgggtgatc acttccttcg ccgtcaccgc cttaccgata
    18061 ctgctgaccc agccgcggaa cacatcgacc gtgccgttcg ggaagcggat tttataggct
    18121 cgggtatccc cttcattaaa ccacgccagc agcgcctgct gcccctgctc tccgggcatc
    18181 cacgccagcg tgaagctggt atctccggca gatttctgcc cctgcccggt cgcagtccag
    18241 tctgcatctt catcatcgag atagctgtcg tcataggact cagcggtcag ttcgccgggc
    18301 gtcaggtctt taacttttgc cagacgcgac cagtcaacgt ctgaaagcgg attcgcataa
    18361 gggtcaccgc ttcccttata aacccacagg gtggtcccgg cacctttcac cggcattaca
    18421 ggatttggta caggcatagc gtcctcacat ttcataggta atgacataag tcagatcggc
    18481 tgaactccac aggcccgcat catcgtcgcg ccggtagtca tagccgctgg ccaccatact
    18541 ggtgatcaaa tctgacagtg ccgggatatc actcatcacc ggataaatcc gggactccat
    18601 ccacgcatcc agctctgaat ccggcacctg agcaggcagg aaaacttcaa tatgcagctc
    18661 cgcctgccat gtatcgctgt ccagctcttc gcccgtgtat tcagcgccgg tgagataaac
    18721 ggcaactgcc ggaaaatccg cctcatcaaa aacagcgggg cgaccatcaa aaaacgtcgc
    18781 cccggtgtca tgcttctcca gtgcatccag tacggctgca cggagttcag tatgtttcat
    18841 cgctttatta ccattctcag ttgatgctgc agcgcatagc ccagctcttt cggaagacgt
    18901 tcacgccgta tccgttcaat attctgttta aacgccgtgg tcagcggcac cgccatcggg
    18961 attttcacca catcaatggg gtaacggttt ttcccggcca cacgctgcat gacatgccac
    19021 cggccatttt tcagttgctg aataaacgcg ccggaaatac gacggtttcc caccacaagc
    19081 acgctgccgc cacctttcag ggatgaacgc tgcccctttt tacgacgtct gcggcgggaa
    19141 aggacaaccc gcgcgttacc cagcttgatt acgggcaaat ccccccggtt aaccctgatt
    19201 ctggcctgcg gatttttgac cgtggccctt ttcagcctgg ccctttcctt taccagtttc
    19261 cggcgtacct ttgtctcacg ggcaacctgt gacgcagact gcgatatcgc ggatgacgca
    19321 acgcggttaa tggccattgc ggcggcacca ggcaccgccg ttttgctgat acggctgagg
    19381 ttttcaacgg cctgctcaag accttttatg gccatacatc cccctttcag cggcgacggt
    19441 taacggcagg cggcacgcca cgcccaagcc agagatgaca gcttccgcca tcatccggcg
    19501 aaatccggtc cacccagaag ttttcctcgc cgatggtcag tgtgtctcca cgccgcagct
    19561 gccgcacatc atcagtccgg acaaacaggg acgggctgga gccttcaacg cgcaccccct
    19621 gtccggcata gctgatattt tcagggtcat caaaaacacc acgtatcaca gcaccggaca
    19681 gctcaccgga tgtcatggtg gctgacgttc ccatgtaccc gcgtatcgtt tcatcggcgc
    19741 gggcaatggc agcatcgaac aggttatcgg aatcagccac agcgcctccc gttattgcat
    19801 tctggccagg ccatgttctg tcatttcggc tgccacaccg gcagagacac gaaacgccgt
    19861 tcccggcagc acaaatgcca caggctcatc ccgcgtggcg tgaagtgcat cagtatgcag
    19921 cgtcaccagt gccacgaccg tgaccagagc agccgtatca atcacggtat ccggctgcgc
    19981 tgataccacc tcattttcat gtccggtcag cgcattttcc gggctgacag atgtgtcctg
    20041 accggcagcg tcatccgtgt catcaagctc ctcttccagc tctgccacac ggagcaccag
    20101 ttcttctttc gtccccgtca ggctgacatc acggttcagt tgctcaccca gcacctgaag
    20161 acgggcaatc agttcatctt tcgtcatgga ctcctccaca gagagaaaat ggccccgaag
    20221 ggccatgatt acgccagttg tacggacacg aacgcatcag gatcagccag cagcatcagc
    20281 ggtgctgact gaatcatggt gaactcacgc gccggatcgc cggtggtcac ccagtttttc
    20341 gggtagcggg cagaggcgtt aataccttcg cgctgtgcgt ccgcatcctg aatgcagcca
    20401 taggtgcgca gaccgcgtgc ctgagtgttc cccagcacca tcgtgttgtc cggcaggaag
    20461 ttctttttga cgtcgttttc cacgtactgt ccggaataca cgacgatcgc cgtatcgcca
    20521 tacatcccct tataggacac cgcttcgccc aggtctttta ccgctgtctc cagctcggaa
    20581 tgagagccgc gacgggtatc cagcttctcc ttgacggctt tgaaggaacg gaacagcgcc
    20641 cagcctttcg gatcaaacac gatgatattc accacgccgc tggcgttcag cgcataggct
    20701 tcgatatcgt cggtcgggtc atacgtggac ttgtcacgct tgctccactc cgtaccaccg
    20761 gactgtgtga tgttgttcgc cgcactgcgg cccatatcca cctcaaccgg atcgaaggct
    20821 tcaccggtca tggtgtattt gcccttaagc acagcagaaa ctgcctgcat ctcttcgacc
    20881 tgagcaatgg ccagctcttc gtctcgcatg ttctgcatga tgatgcgacg gcggcggtaa
    20941 gccgggtccg ccagattctg cggatcttca tccggcaggc gacgcagggt catctgcgga
    21001 ttcacctcat gcttgggttt gacatatccc ggcgtaaatt cagaggtgga gccgccacgg
    21061 gaacggataa cctcaccgga aacaatcggc gaaacgtaca gcgccatgtt taccagtccc
    21121 ggaatttgtg agagatagac tttctccgtg gtgaagggat agctctcacg gaaaaagaga
    21181 cgcagaaaca gcggatcaaa cttaaatttc tgctcatttg ccgccagcag ctgggcggtt
    21241 gtgtacatcg acataaaaaa atcccgtaaa aaaagccgca caggcggcct ttagtgatga
    21301 agggtaaggt taaacgatgc tgattgccgt tccggcaaac gcggtccgtt ttttcgtctc
    21361 gtcgctggca gcctccggcc agagcacatc ctcataacgg aacgtgccgg acttgtagaa
    21421 cgtcagcgtg gtgctggtct ggtcagcatc aaccgccaga atgccaacgg cagtaccgtc
    21481 ggtggtgcca tcccacgcaa ccagcttacg ggtggaggtg tccagcatca gcggggtcat
    21541 tgcaggcgct ttcgcactca atccgccggg cgcggttgcc gtatgtgccg ggtcactgtt
    21601 gcccagcggc tggtaatggg taaaggtttc tttgctcgtc ataaacatcc cttacactgg
    21661 tgtgttcagc aaatcgttaa cggcatcaga tgccgggtta cctgcagcca gtggtgccgg
    21721 tgccccctgc atcagacgat ccagcgcagt gtcactgcgc gcctgtgcac tctgtggtgc
    21781 agctgccaga atgcggcggg ccgtttccac ggtcataccg ggggtttcgg ccagcacacg
    21841 tgcctgttct tcgcgtccgt gagcttcctc acagttgagg atccccataa tgcggctgtt
    21901 ttctgccgca accgcagcgg tgatctgcgc gttcacgtcc ggctgcgccg cgctggcgtt
    21961 ttcgccctcc gtcgcttgca ccacgtcagt aacgtcagcc tgcgaagcag tggctgaaac
    22021 agttgttgat tgagtctctt tggtcattcg ccctcctgag agacgggatt tacgtgcatc
    22081 cagtgcatca cgcataacgg tgatcgcatc ggtgctgttg acaagttcat cagccagtcc
    22141 ggcatcaatg gcctcctgac cgctgtacac tgcagcctcg gtatccagca cagcctgcac
    22201 ggacaggccg gtatatgccg acaccttctg cgcaaacatc cggcgggttg catccatccg
    22261 ggactgcagt gtctcccgga catcacccgg tagatggctg taggggttgc catccacctt
    22321 atggctgccg ctgtaaatca gcgtgatttc cacgccctgt ttctccagcg cagcgccgta
    22381 attactgtga gccatcatga cgccgatgga gcctgtccgg gcggtctgcg tgaccagacg
    22441 ccgggaggcg gcgctggcaa gcagctgacc tgcactgcag ttcatgtcgt tggccagcgc
    22501 ccataccggc tttatgtcac gcacacgggc gatgatgtca gcacagtcaa atgctcccgc
    22561 caccatcccg cccggtgtgt ccatatcgag cagaatgccg tccaccatcg gatcgctggc
    22621 agcctgttgc agacgggcga taatgccgtt gtaaccggtc attcccgaat acggctgcag
    22681 cgcccgcgtc cggctgacca gcgtaccgga caccggcagc acggcgatgc cgttcatgac
    22741 ctgataactg cgggcctgtc gtggtccgtc atcatcaccg gataacgcca gcgccgcggg
    22801 tgcctctccg gcagtcaggc tgtcgccgga tactgcatcc gtcaggcgac tgatcccaag
    22861 ctggcctgca agcgcacaaa agaaaacccg cgcataggcg ggttcaagca tcagcggctc
    22921 attaaaagcc atgctggcaa tatgcgggag attacgcagc tctgctgtca ctcttctcct
    22981 cctctgttga ttgtcgcagt ccggattcaa atgccgcagc cgcccaggcg ggcggtttaa
    23041 gaccggctgc acggcgctcc atcgtttcac ggacctgctg ggcaaaaatt tcctgatagt
    23101 cgtcaccgcg ttttgcgcac tctttctcgt aggtactcag tccggcttct atcagcatca
    23161 ccgcttcctg tacttctttc agaccatcga tggccatacg accggagcct atccagtcac
    23221 agttccccca ggcgctgcgg gcttcctgaa agctgaaacg cgcttttgaa ggtaacgtca
    23281 ccacgcggcg aacgatggcc tcttccagcc agcacagaaa catctggctc gcctgacggg
    23341 atgcgacgaa ttttcgccgc cccataaagt acgcccacga ctcgttcgca ctggcccgtg
    23401 ccgtggagta gctcatctgg gcgtaattcc gggaaagctg ctcatacgag acacccagcc
    23461 cggcagcgat ataccgcaac agtgactgtt caaacacgga gtagccgtta tccgtgtcct
    23521 gagccgtctg caggttcagt gagtcccccg gcatcaggtg cggcactttt gcgcctccca
    23581 gacggaccgg tgctgcggcg taatacgcgg caatttcacc aatccagccg gtcagccttt
    23641 cccgctgctc ctgactgttc gcgcccagaa taaaatccat cgctgactgc gtatccagct
    23701 cactctcaat ggtggcggca tacatcgcct tcacaatggc gctctgcagc tgcgtgttct
    23761 gcagcgtgtc gagcatcttc atctgctcca tcacgctgta aaacacattt gcaccgcggg
    23821 tctgcccgtc ctccacgggt tcaaaaacgt gaatgaacga ggcacgcccg ccgggtaact
    23881 cacggggtat ccatgtccat ttctgcggca tccagccagg atacccgtcc tcgctgacgt
    23941 aatatcccag cgccgcaccg ctgtcattaa tctgcacacc ggcacggcag ttccggctgt
    24001 cgccggtatt gttcgggttg ctgatgcgct tcgggctgac catccggaac tgtgtccgga
    24061 aaagccgcga cgaactggta tcccaggtgg cctgaacgaa cagttcaccg ttaaaggcgt
    24121 gcatggccac accttcccga atcatcatgg taaacgtgcg ttttcgctca acgtcaatgc
    24181 agcagcagtc atcctcggca aactctttcc atgccgcttc aacctcgcgg gaaaaggcac
    24241 gggcttcttc ctccccgatg cccagatagc gccagcttgg gcgatgactg agccggaaaa
    24301 aagacccgac gatatgatcc tgatgcagct ggatggcgtt ggcggcatag ccgttattgc
    24361 gtaccagatc gtccgcgcgg gcattgccac gggtaaagtt gggcaacagg gctgcatcca
    24421 cactttcact cggtgggttc cacgcccgca actgcccacc aaatccgctg ccaccgccgt
    24481 gataaccggc atattcacgc agcgatgtca tgccgtccgg ccccagaagg gtgggaatgg
    24541 aggacgtttt catacataaa atcctgcagg tcccctgcgt cgctgtgtca tgccggtctg
    24601 cacttccagc tccgcaatgt attttttcag gtcagacacg gaagtggccg taaactccac
    24661 tcgccgtccg tctttctgta ccgttgccac ccgttttcct gtcatcaggt catgcagtgc
    24721 cgcacgggca gcggcaagtt cttcctgtcg cgtcattcat cctctccgga taaggcacgg
    24781 gcgtaatctg ccagtgtttt cttgttggtt gctgcaccat cctcttcctg caggctcgcc
    24841 agcagtgcac tgagatccag ctgccagcgg gaaatactga tgcgcagcgc cgccagcgca
    24901 taaacgaagc agtcgagcgc ctcattgcgt cgctttttgc tgtcccacag tatttttttc
    24961 ctgccatcca cccatttttc gacctgctct tcagcagtca gctgctgcgc ttcggtcaga
    25021 tcaaaaatat ccgggttatt cgggaagtga acggcaccgg gaagcggttc atccccttcc
    25081 ggcgtcagtg tgaagcggtt ataaatctgc tctttcgcgg tatccgtacc gatttcggta
    25141 aggtaaaccc cgtttttgtt tcgcttacgt ggcatgctgg ccactggctt tccgtagacg
    25201 gatgcccctt taatggggat cacccggaac agcccatgct ttttcgagcg ttcatacaca
    25261 atggtcgggt caatcccgcc agtatcccag cagatacggg ataccgacat ttctgcacca
    25321 ttccggcggg tataggtttt attgatggcc tcatccacac gcagcagcgt ctgttcatcg
    25381 tcgtggcggc ccataataat ctgccggtca atcagccagc tttcctcacc cggcccccat
    25441 ccccatacgc gcatttcgta gcggtccagc tgggagtcga taccggcggt caggtaagcc
    25501 acacggtcag gaacgggcgc tgaataatgc tctttccgct ccgccatcac ctcagcatcc
    25561 ggacgttcgc caattttcgc ttcccatgtc tcaccgagcg tggtgttcac gaaggtttta
    25621 cgttttcccg tatccccttt cgtcttcatc cagtctttga caatctgcac ccaggtggtg
    25681 aacgggctgt acgccgtcca gatgtgaaag gtcacactgt ccggcggctc aatctcttca
    25741 ccggatgacg aaaaccagag aatgccatca cgggtccaga tcccggtctt ttcgcagata
    25801 taacgggcat cagtaaagtc cagctcctgc tggcggatga cgcaggcatt atgctcgcag
    25861 agataaaaca cgctggaggg gtcatccggc gtccatttga ggccaaacgg cgtctctttg
    25921 tcgccaaatt taagatactg ctcctccccg cagtgcgggc aggcaacatg aaaacgcata
    25981 aaatgcgggg attcactggc tgcacgctca atctggcagg tgcctctcac tttgggcgtg
    26041 gagccacgga tggactttgg ccagaccgag ccttcaatac gtttgtcgcc aaggaacgtc
    26101 ggagagcctt cctgttcaat atcctcatca aaggcagcaa gttcatcata acccgccaca
    26161 tccaccgact tttcacggta gttttttgcc gctttaccgc ccaggcacca gaagccacga
    26221 ccattggaaa aacgcttcat agtgagcgtg ttatcccggt gctttttgcc ataccacgga
    26281 gccagcgcca gcagcgacgg aatatcgcgg atggtcggct caacgtgggt tttcataaag
    26341 ttctcggcat caccatccgt cggcaaccag ataagggtgt tgcgctgctt atgctctata
    26401 aagtaggcat aaacacccag cagcattttg gaataaccga cacgggcaga cttcaccaca
    26461 ttcacctcac ggatgtagtc gctgcccatc gcattcatga tggcccgctg aaagggcagt
    26521 gtttcccagc gcccttcctg gtatgcggat tcttttggga gatagtaatt ggcatccgcc
    26581 cattcaacgg cggtctgtgg ctccggcctg aacagtgagc gaagcccggc gcggacaaaa
    26641 tgccgcagcc tgttaacctg actgttcgat atattcactc agcaaccccg gtatcagttc
    26701 atccagcgcg gctgctttgt tcatggcttt gatgatatcc cgtttcagga aatcaacatg
    26761 tcggttttcc agttccggaa aacgccgctg caccgacagg gggatcccgt cgagaatact
    26821 ggcaatttca cctgcgatcc gcgacagcac gaaagtacag aatgcggttt ccaccacttc
    26881 agcggagtct ctggcatttt tcagctcctg tgcgtcggcc tgcgcacgcg taagtcgatg
    26941 gcgttcgtac tcaatagtcc ctggctggag atctgtctcg ctggcctgcc gcagttcttc
    27001 aacttcccgg cgcagctttt cgttctcaat ttcagcatcc ctttcggcat accattttat
    27061 gacggcggca gagtcataaa gcacctcatt acccttgcca ccgcctcgca gaacgggcat
    27121 tccctgttcc tgccagttct gaatggtacg gatactcgca ccgaaaatgt cagccagctg
    27181 ctttttgttg acttccattg ttcattccac ggacaaaaac agagaaagga aacgacagag
    27241 gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt attttaaata
    27301 aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa ttttcataaa
    27361 tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcgccggaa aggacccgca
    27421 aaaatgataa taattatcat ctacatgtca caacgtgcat ctacgccatc aaaccacgtc
    27481 aaataatcaa ttatgacgca ggtatcgtat taattgatct gcatcaactt aacgtaaaaa
    27541 caacttcaga caatacaaat cagcgacact gaatacgggg caacctcatg tcaacgaaga
    27601 acagaacccg cagaacaaca acccgcaaca tccgctttcc taaccaaatg attgaacaaa
    27661 ttaacatcgc tcttgagcaa aaagggtctg ggaatttctc agcctgggtc attgaagcct
    27721 gccgtcggag actaacgtca gaaaagagag catatacatc aatccaaagt gatgatggat
    27781 gaacatcccg gtttcttcca ccatcgcacc ggaaaagcga ctatgagggt aaccctgcgt
    27841 ctgtcagcac agtaaaaccc ggtgtgcatc gtttttgatt attcccgcac actcacgcag
    27901 aaggaattcc ccgtcgggct acggtcatgg ttaatgcggg aatacggcga cgatacagcg
    27961 cagctaaaag ggtaatggac agatagagcg gtttatttca ttccacagga ttctgagtgc
    28021 cccccctcct ccaataggct gagcatccac ctatatagtt ttaattttca tcaatccatt
    28081 taactatcgt ttaattgttg tcacatagga ttctgccgtt tttaacaatg caggataata
    28141 agatgaaaaa aatgttgttt tctgccgctc tggcaatgct tattacagga tgtgctcaac
    28201 agacgtttac tgttggaaac aaaccgacag cagtaacacc aaaggaaacc atcacccatc
    28261 atttcttcgt ttcgggaatt ggacaggaga aaactgttga tgcagccaaa atttgtggcg
    28321 gcgcagaaaa tgttgttaaa acagaaaccc agcaaacatt cgtaaatgga tttctcggtt
    28381 ttattacttt aggcatttat actccgctgg aagcgcgtgt gtattgctca caataattgc
    28441 atgagttgcc catcgatatg ggcagctcta tctgcactgc tcattaatat acttctgggt
    28501 tccttccagt tgtttttgca tagtgatcag cctctctctg agggtgaaat aatcccgttc
    28561 agcggtgtct gccagtcggg gggaggctgc attatccacg ccggaggcgg tggtggcttc
    28621 acgcactgac tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcatc
    28681 acgcagagca tcattttcag ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag
    28741 cgcagcaaca tcacgctgac gcatctgcat gtcagtaatt gccgcgttcg ccagcttcag
    28801 ttctctggca tttttgtcgc gctgggcttt gtaggtaatg gcgttatcac ggtaatgatt
    28861 aacagcccat gacaggcaga cgatgatgca gataaccaga gcggagataa tcgcggttac
    28921 tctgttcatt gctgacccca caaacagatt tcacgctcaa tctcacgacg agtcatgaga
    28981 cctttccatt gcttaccgcc agcatatgtc cagcgacgta gctgatcaca tgcgcctttg
    29041 atatcgccct ggtttatttt gcgaagaagc gtcgatgttc tgaaattgcc agcacccacg
    29101 ttgtaaacga atgagtaaag agcgccgcgc attgtttccg gtatatcgac tttgatgtac
    29161 gggttaattt gtctggcgac cgtggcaagg tctttattca ggagggcttt gcattctgct
    29221 ttggtatacg ttttaccgag catgatgtct tttcctgtat gcccgtgaca tacagtccat
    29281 acaccaacaa tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccactt
    29341 gggccagtga ttaacactga tgctatagca attgctccgc caccaatagc agcagcaacg
    29401 gcttttcgta atgatggagg cattattcac ctctcgcagc cttgcgctta tcttctttaa
    29461 tcttgaaata aaggtttgtc aggtacgtca gcaggccaaa taccaggcta cccagcacac
    29521 ctattgctgc ccactgtgag ggcgtgactt tatcgagcag ctgtaaaaac cagtaaccgg
    29581 cactacctgc tgaggtgcca taggcgacac ccgttgttaa cttatccatg gatttcataa
    29641 ccccacctcg cagacaaagc gggtgtaaat tgagggaata caacgtatcg caaaaaagca
    29701 gaaacgtaac agactcggag tcagtgaata actcaggtat tgggttatca gctaatatcg
    29761 agactcaaaa aatggaaaaa cccgctcgac ggcgggttta agctgtgtga cgaagtaacc
    29821 actcttaaca gcataaccaa ttttttacgt acgtaaacca caaaatgata tttgcgagaa
    29881 tgctaccgag tattgaaaac accactacaa atacataagc aaatctcaac aaataaccaa
    29941 caaataattt ccggtgttat ttttagccga tttaaattga accttcaaat tacagagcac
    30001 ttataaataa cagccgttaa tataaat
//
LOCUS       DAZGTJ010000062        29889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000062 DAZGTJ010000000
VERSION     DAZGTJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.062"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            320..1012
                     /gene="yieP"
                     /locus_tag="ACHK8E_003295"
     CDS             320..1012
                     /gene="yieP"
                     /locus_tag="ACHK8E_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP0345519.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            1035..2462
                     /gene="mdtD"
                     /locus_tag="ACHK8E_003296"
     CDS             1035..2462
                     /gene="mdtD"
                     /locus_tag="ACHK8E_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP0345520.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2428..3420)
                     /gene="rbsR"
                     /locus_tag="ACHK8E_003297"
     CDS             complement(2428..3420)
                     /gene="rbsR"
                     /locus_tag="ACHK8E_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP0345521.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3424..4353)
                     /gene="rbsK"
                     /locus_tag="ACHK8E_003298"
     CDS             complement(3424..4353)
                     /gene="rbsK"
                     /locus_tag="ACHK8E_003298"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0345522.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4479..5369)
                     /gene="rbsB"
                     /locus_tag="ACHK8E_003299"
     CDS             complement(4479..5369)
                     /gene="rbsB"
                     /locus_tag="ACHK8E_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP0345523.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5394..6359)
                     /gene="rbsC"
                     /locus_tag="ACHK8E_003300"
     CDS             complement(5394..6359)
                     /gene="rbsC"
                     /locus_tag="ACHK8E_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0345524.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6364..7869)
                     /gene="rbsA"
                     /locus_tag="ACHK8E_003301"
     CDS             complement(6364..7869)
                     /gene="rbsA"
                     /locus_tag="ACHK8E_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP0345525.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7877..8296)
                     /gene="rbsD"
                     /locus_tag="ACHK8E_003302"
     CDS             complement(7877..8296)
                     /gene="rbsD"
                     /locus_tag="ACHK8E_003302"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP0345526.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8463..10331)
                     /gene="kup"
                     /locus_tag="ACHK8E_003303"
     CDS             complement(8463..10331)
                     /gene="kup"
                     /locus_tag="ACHK8E_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP0345527.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10554..12050
                     /gene="ravA"
                     /locus_tag="ACHK8E_003304"
     CDS             10554..12050
                     /gene="ravA"
                     /locus_tag="ACHK8E_003304"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP0345528.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12044..13495
                     /gene="viaA"
                     /locus_tag="ACHK8E_003305"
     CDS             12044..13495
                     /gene="viaA"
                     /locus_tag="ACHK8E_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP0345529.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13500..14492)
                     /gene="asnA"
                     /locus_tag="ACHK8E_003306"
     CDS             complement(13500..14492)
                     /gene="asnA"
                     /locus_tag="ACHK8E_003306"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP0345530.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14644..15102
                     /gene="asnC"
                     /locus_tag="ACHK8E_003307"
     CDS             14644..15102
                     /gene="asnC"
                     /locus_tag="ACHK8E_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP0345531.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15192..15635
                     /gene="mioC"
                     /locus_tag="ACHK8E_003308"
     CDS             15192..15635
                     /gene="mioC"
                     /locus_tag="ACHK8E_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP0345532.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            16014..17903
                     /gene="mnmG"
                     /locus_tag="ACHK8E_003309"
     CDS             16014..17903
                     /gene="mnmG"
                     /locus_tag="ACHK8E_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP0345533.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17967..18590
                     /gene="rsmG"
                     /locus_tag="ACHK8E_003310"
     CDS             17967..18590
                     /gene="rsmG"
                     /locus_tag="ACHK8E_003310"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP0345534.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVVIKANKI"
     gene            19207..19587
                     /gene="atpI"
                     /locus_tag="ACHK8E_003311"
     CDS             19207..19587
                     /gene="atpI"
                     /locus_tag="ACHK8E_003311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP0345535.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            19596..20411
                     /gene="atpB"
                     /locus_tag="ACHK8E_003312"
     CDS             19596..20411
                     /gene="atpB"
                     /locus_tag="ACHK8E_003312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP0345536.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            20458..20697
                     /gene="atpE"
                     /locus_tag="ACHK8E_003313"
     CDS             20458..20697
                     /gene="atpE"
                     /locus_tag="ACHK8E_003313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP0345537.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            20759..21229
                     /gene="atpF"
                     /locus_tag="ACHK8E_003314"
     CDS             20759..21229
                     /gene="atpF"
                     /locus_tag="ACHK8E_003314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP0345538.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            21244..21777
                     /gene="atpH"
                     /locus_tag="ACHK8E_003315"
     CDS             21244..21777
                     /gene="atpH"
                     /locus_tag="ACHK8E_003315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP0345539.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            21790..23331
                     /gene="atpA"
                     /locus_tag="ACHK8E_003316"
     CDS             21790..23331
                     /gene="atpA"
                     /locus_tag="ACHK8E_003316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP0345540.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            23382..24245
                     /gene="atpG"
                     /locus_tag="ACHK8E_003317"
     CDS             23382..24245
                     /gene="atpG"
                     /locus_tag="ACHK8E_003317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP0345541.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            24272..25654
                     /gene="atpD"
                     /locus_tag="ACHK8E_003318"
     CDS             24272..25654
                     /gene="atpD"
                     /locus_tag="ACHK8E_003318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP0345542.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            25675..26094
                     /gene="atpC"
                     /locus_tag="ACHK8E_003319"
     CDS             25675..26094
                     /gene="atpC"
                     /locus_tag="ACHK8E_003319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP0345543.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            26443..27813
                     /gene="glmU"
                     /locus_tag="ACHK8E_003320"
     CDS             26443..27813
                     /gene="glmU"
                     /locus_tag="ACHK8E_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP0345544.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            27975..29804
                     /gene="glmS"
                     /locus_tag="ACHK8E_003321"
     CDS             27975..29804
                     /gene="glmS"
                     /locus_tag="ACHK8E_003321"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP0345545.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         7377 a         7627 c         7676 g         7209 t
ORIGIN      
        1 ctcagcggcg cggggtgtgc ataatacgct ttcccgctac agagtcaagc atttgttttt
       61 gctttctctg ccggaattct caggagaacc ccgctaaccc ggcggcgtgt ttgccgttgt
      121 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagag gaaatttaaa
      181 ataattttct gaccgcgcat tttttcagca aaagccccgt taatgtgcca taaaacaagc
      241 aatttgccgt ttttgcaacc gtaatcacac ttcctggtgg catactaagg aggaataaaa
      301 agaaaaagga agattagtaa tgcctttaag cgcacaacag ttagctgcac agaaaaacct
      361 ttcgtatgtt ctggctgaga agctggcgca acgcatctta aaaggtgaat atgaacccgg
      421 caccattttg cctggtgaga ttgagctggg cgagcaattt ggagtgagtc gtacagcggt
      481 acgcgaagcg gtcaaaacgt taacggcaaa agggatggtt ttaccgcgac cgcgaattgg
      541 tacccgggtc atgccacaat caaactggaa ttttcttgat caggaattgc ttacctggtg
      601 gatgacagaa gagaactttc atcaggtcat cgatcacttt ctggttatgc gtatctgtct
      661 ggagccgcaa gcttgcctac tggcagcaat ggttggcacc gcggaacaga aggcgcatct
      721 caatacgtta atgaccgaaa tggtggcatt aaaagagaac tttcgccgtg aacgctggat
      781 tgaggtcgat atggcctggc atgagcatat ctatgaaatg agcgccaatc cgtttttgac
      841 ctcattcgcc tcgctcttcc attcggttta tcacacttac ttcacgtcaa ttaccagcga
      901 cacagtgata aagctggatc tgcaccaggc gattgttgac gcgattatcc aaagcgatgg
      961 cgacgcggca tttaaagctt gtcaggcgct gctacgctca cctgataagt gacaccccga
     1021 taacaggaca ttgaatgagc gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg
     1081 cggcgatggc cttctttatg caggcacttg atgccactat tctgaatacc gccttacccg
     1141 caatcgctca tagccttaat cgttctcctc tcgcgatgca atcagccatc atcagttata
     1201 cgctgacggt ggcgatgctt attccggtaa gcggatggct agccgatcgc ttcggtacgc
     1261 gtcgcatatt tactcttgcc gtgagtctgt tcacgttggg ttctctggcc tgcgcacttt
     1321 ctaactcgct accacagctg gttgtcttcc gggttattca ggggataggc ggcgcaatga
     1381 tgatgcctgt tgctcggctg gccttactgc gcgcttatcc tcgtaatgag cttcttcctg
     1441 tattgaattt tgtcgccatg ccgggtctgg tggggccaat tttaggccct gttcttggcg
     1501 gcgtgctggt cacctgggca acctggcact ggatattttt aatcaatatc cccataggta
     1561 ttgcgggcct tctttacgcg cgcaaacata tgcccaattt caccaccgca cgacgcagat
     1621 tcgatatcac tggctttttg ctgtttggcc ttagccttgt tctcttctca agcggaatag
     1681 agctattcgg ggaaaagatt gtcgccagtt ggattgcctt gacggtaatt gtcaccagca
     1741 tcgggttact gcttctctat attctccatg cacgacgcac gccaaaccca ttaatttcat
     1801 tagatttatt taaaacccgc actttctcga tcggtatcgt aggcaatatt gcaacccgtc
     1861 tggggaccgg ttgtgtaccg ttccttatgc cattgatgtt acaggtagga tttggttatc
     1921 aggcgtttat tgccggctgt atgatggcac cgacagcgtt aggttccatt attgcaaaat
     1981 cgatggttac ccaagtctta cgtcgtctgg gctatcgcca tacattagtg gggatcacgg
     2041 tgattattgg gctaatgatc gctcagttct ctttgcaatc accggcaatg gcgatatgga
     2101 tgctgatctt gccgttgttt atattaggga tggctatgtc gacgcaattt accgcgatga
     2161 ataccatcac acttgccgat ctgaccgatg acaacgccag cagcggtaac agtgttctgg
     2221 cggtcacgca gcaactgtct atcagtttag gcgttgctgt aagtgcggcc gtccttcgcg
     2281 tttatgaagg gatggaaggc acaacgactg tcgaacaatt ccactatacg tttatcacga
     2341 tgggcattat tactgttgct tcagcagcaa tgttcatgct tctgaaaaca accgatggta
     2401 ataatttgat caaaagacag cgtaaatcta agccgaaccg cgttccatca gaatcggagt
     2461 aagttgtaat cgttgttgct gaagggtcgg ttgggttatc cgatggatga gtacatcaat
     2521 cgccagctcc cctagttcat ctttcggttg gtggatagtg gttaatggtg gcgtcataaa
     2581 gcttgccagt tcgatatcgt catagccaat caccgcaata tcctgcggaa cttgtaactc
     2641 tgcctgatac agcgcctggt aaacgccaac agccatagcg tcatttccgg taaagacggc
     2701 ctgaggacgc agcggatgta ataacagttg gcgcatagcg tcaaacccgc cgttaaattc
     2761 aaaatcacca gtgacttcat agccatcagg aatgttgaga cccgcacgtt tcatcgccgc
     2821 ccgataacct tccaaccgca ggcgtgccgg agttttatcc agcgggccgg taatacaggc
     2881 gatacgggta tgacctttat cgatcagata ttgcgttgct aagtctccgc ccagcaacga
     2941 gttatcctga ataagatcgc tgtcgccatc gaacggagcc cagtccatca tcacagtagg
     3001 cactgtcgga taacgttgca tgatttcacg cgaaggttga tgcgtttcgg tgcacagtaa
     3061 cagcaagcca tcaacgcgtt tttgcatcag cgtttccaga ttgcgattca tccgctgttc
     3121 atcgccttcg gtattgcaaa ggacaagact ataaccgcgt tcgaagcaac tgcgttcaac
     3181 gccacgcacc agttcagaat agaaaggatt ggtactggca gtgatcaaca tgccaatggt
     3241 atgtgtttga ttgagtttga ggctacgcgc cagggctgat ggcgcgtaat tgagttcttt
     3301 aatcgccgct tcaactttgg cggtaatcgc ttcactgacg aagcgatctt tattgataac
     3361 gtgagaaact gttgaggtag aaacgcccgc caggcgggca acatctttca ttgtagccaa
     3421 gcgtcacctc tgcctgtcta aaaatgcgtc gatctcttca cgccacggta cggaaggttg
     3481 tgcgccttta cgtgttacgg caatcgcagc ggcagcatga gcaaaccgaa tcgcctctgg
     3541 caatggtttt tcttccagca acgccgtgat taacgcgccg ttaaaggtat ctccggcagc
     3601 aatggtatcg acagcctgca cccggaatcc aggaacgcgc tgaccttcac cattcacgct
     3661 agcccataca ccacgacttc ctaaagtaat cagtacagta cggatacctt tttcatgcag
     3721 tacctgcgcc gccttcgctg catcttcatc attttcaaca cgaataccgg tgagcttttc
     3781 tgcttccgtt tcgtttggcg taataatgtc caccagtgcc agcagttcgt caggaagttc
     3841 gcgagccgga gccgggttaa gcgcaacgat agtcttattt tgatgggcga ttttcgccgc
     3901 tgccatcaca ctttcgagtg gtgattccag ctgcattaat aatgctgacg cgttggcaat
     3961 acgctcacgt tgcgcttcca ccagtgccgg ggaaagggca gcattagcgc cggcatgaat
     4021 accgatgaca ttctcacctt cgccattaac aaaaatcagc gccacacctg ttgattcgcc
     4081 tttgatcacg ctgaccggag aaatatcgat gttatcagtg gcgagctgct ggcgaacgct
     4141 ctcaccaatg ctgtcatcgc cagtgcaggc aataaacgcg atattcgcac cgctacgccc
     4201 agcagccaca gcctgattag cgcctttgcc gccaaatgca acctgatagt ggttaccggt
     4261 tacggtttcg cctggggtag gaaaagattg aagattaaga atgtggtcag cattaatgct
     4321 gccaagaaca acgaggctgc ctgcgttttg catattcggg atgtccataa aatgcgccac
     4381 cgtgttaggg tggcgcgtgc cctgcttttc tttatattac acgttcatct gtcgatgccg
     4441 tatttatgtc accatcaggt catacaacct gattaaaact actgcttaac aaccagtttc
     4501 agatcaaccg gatacttagc ctgaactttc tcacctttca gtactttatc ggcggtttcg
     4561 acgcctttcg cgccaatctg atcgggtagc tgagcgatag tcgctgctag tttgccatca
     4621 ttcaccgctt tttcgccatc cggtgtaccg tcaaatccga cgaccatcac atccgattta
     4681 ccggcagttt gcagtgcgcg cagcgcgccc agcgccattt catcattctg cgcgaataca
     4741 gcctgaacat ccggatgtgc ggtcaacagg ttctgcatta cgttcaaacc tttagtgcga
     4801 tcaaaatctg ctggctggct ggcaagaaca ttaaacttgt gagcagcaac agcctgctgg
     4861 aagccttcgc cacgttcacg ggctgcggat gtaccggcaa tgccttgcag ctcgataact
     4921 ttggcacctt cacccgcttt cttcgcgatg taatcaccgg cgattttgcc gcccagtacg
     4981 ttatcagaag caatgtggct caccacttca cctttcgttg cctggcggtc aagagtgata
     5041 accgggatgt tcgcctggtt agccatcttc acagcattac ccactgcgtc ggagtcggtc
     5101 gggttaatca gcagaatttt ggtgccgcga acggttaagt cctgcacgtt cgccagctct
     5161 ttcgccgggt tattctggga gtccagcacc accaggttat agccaagttt atccgcctct
     5221 ttctgcgcgc catctttcag cgatacaaag aacgggttgt taagcgtgga gaccaccagc
     5281 gcgatggtgt cttttgccat cgcattcgca ctgacggtgg cgcttagcgc aacagcggaa
     5341 accagggtag ccagtttttt catgttcata ttcaagatgt cctgtagtcg ttattactgc
     5401 tttttgttgt ctaccagcac cgccagcaaa atcaccaccg ctttgacgat catctggtaa
     5461 taggaggaaa cacctaacaa attcaatcca ttattaagga agccaagaat taatgcgccg
     5521 atcaacgtcc caacaatgcg accttttccg cccgccagac tcgtaccgcc cagaaccacc
     5581 gcagcaatgg catccagctc atagccagtc cccgccgtag gttgtgcgga ggagagacgc
     5641 gccacttcaa tgatcccggc cagcgatgcc agcagaccac aaagagaata gacgatgatt
     5701 ttgattttat tgacgttgat accagaaagg cgcgttgccg cttcgttgcc gcccagcgcg
     5761 tagatgtaac gccccagacg cgtgtgatgc agcatgtacc aggccgcgag gaagacaatt
     5821 cccatgatcc agactggcgt cggtacaccc agcggacgac caataccaaa ccagccaaac
     5881 agatcggcgt tctcagtaaa gccagtattc actgggctac cgttggtata aaccatggtc
     5941 acgccgcgca gtaaaagcat cataaccagc gtagcgataa aggcctggac gcgacctttc
     6001 gctacaatca ccccggttac cgcaccaatt gcggcaccta acgcgagagc ggcagcgaca
     6061 gccaccagcg cattgacttc aatgccaacg atagatgcag caactgcgcc ggtcagcgcc
     6121 aatagagaac ctaccgacaa gtcgatgccc gacgtcagga tcaccagcgt catcccgact
     6181 gccataatgg cgttcaccga ggtttgctgg agaatattga ataagttatt gatggtgaaa
     6241 aagttcgggc ttaacgtcga gacaatcgcg atcagcacca gcagagcgat aagcgatttc
     6301 tgctccatca gccacgcttt cgtgaaataa cggcgaccag agacagtctg ggttgtcatt
     6361 tttttactcc tgattcacgc gattaagctt gcccacagcg gcagccatta acacttcctg
     6421 ggtggcctgc tcacgagtaa attccccgct gagatgccct tcatgcatga cgatgatgcg
     6481 atcgctcatg cctaatactt ctggcatctc cgatgacacc agaatgatgc tcaagccatc
     6541 ggctttgaac tggttaatca gttgatagat ctcttttttc gcgccgacat ctacgccacg
     6601 ggtaggctca tcgaggatca acactttggg gcgtgtcatc agaccacggg caatcgccac
     6661 tttttgctga ttgccaccgg agagcagacc aattgcctgt tccatcgacg gcgttttcac
     6721 attaaacaga cgaatgaaat cacttactgc ctgctgttca tcggcatgct tcaaactgcc
     6781 gccagcgcgg ctgaagtagc gcagcgctgt cagcgacatg ttctctttta ctgacatgcc
     6841 cagcactaaa ccgtcacgtt tacggtcttc ggagatatac acaatgccgt ttgccaggcc
     6901 atcctgcggt gaacgggtaa cgacttcatg cccatccagg gtgacgtaac cgctggtgcg
     6961 cggcagtgcg ccgtagagca ctttcatcag ttcggtacga cctgcgccca tcaaaccaga
     7021 gacgccaaga atttcgcctt tgcgtaaagt aaaagagaca tcgttaacgc caggtccgca
     7081 gagattatcg actttcaggc ggatatctcc cggcgctttg tccaggtgcg gatattgatc
     7141 ttccagcttg cgacccacca tcatctcaat cagcgaatct tcggtcagtg atgctacttc
     7201 acgctcagca ataaattgcc catcacgaaa aacggtaacg tcatcgcaaa tctcgaagat
     7261 ttctttcatg cggtgggaga tatagacaat accgcggcct tgcgatttca gctcgcggat
     7321 gacgcggaac aaggattcgg tttcggtatc ggtcagcgca tcggtcggtt catccataat
     7381 gatgactttc gactcaaagc tcagcacttt ggcaatttca accatttgct ggtcaccgat
     7441 ggaaagatcg cccaccagct tgtcgctttt aaagcgcagg ttaagtttag ccagcaattt
     7501 atccgcttcg gcatacatgg ttttccagtc aattttgcca aagcgattaa caaactcacg
     7561 accgaggaaa atgttttcgg caatggtcaa ctgcgggatc aggttcagtt cctgatggat
     7621 aatcccaatc ccggcttcct gggaagattt cggcccggta aacgtcgttt ctttccccag
     7681 ccataaaagc gtaccggcat cgcgagtata gatacccgta agcactttca tcatggtgga
     7741 tttacccgca ccgttttcgc ccaccagcgc catcacgcgg ccaggataga catttaacgc
     7801 tgcgcccgag agggctttta cgcccgggaa ggctttatcg atgcctttaa gctgaagtaa
     7861 tgcttccatg acggcctcag aacgtcacgc cagcacagag aatgatattc gcatacggag
     7921 aacattctcc gctgcgaatt accgcctgac tttctgcggt ttgttgtttg aattgttcat
     7981 gcgtggtgta acgaatttca atggtatttc cctggtgttt ttgcagctgc tcaaggtgag
     8041 tgagcaacgt ttcgtggaga tgcggattat gttgtttgat ctcttccgcg ataatggctg
     8101 cctcgacctg catttcattt gtgacgacgc ccagcacctg cataaaagaa ggtacgccct
     8161 gggttaatgc catatcgata cgcgttgtac ttttggggat gggtaaacca gcatcacaca
     8221 ccaccagcgt atcggtatgt cccagacggg agatcaccga tgaaatatca gaattaagaa
     8281 cggtgccttt tttcattttt ttctccatca gcgaaacgtt tcgctgaccc gcagtttaat
     8341 cctgactgag ttgggaaaac catcgtgacg ttacggaaat gtgatcaacc tcgaaacgtt
     8401 ttacatggta attaaccatg aaaacagaaa cgccccctta aggagaaagg aggcgtctgg
     8461 cgttagattt cgacctgagt acccagttcg ataaccctgt ttggcgggat ttcaaattga
     8521 tccggcgcac gcagcgcatt acgttgcagc agcaagtaca gcttgccgcg cagacgcaaa
     8581 taccacgggc gtttgccgag gatcaacgac tcatgcgaca taaagaagga ggtttccatc
     8641 atccggcaac ttaatccttc cagaccgcag cggtggaaaa cttcttccac gtttggcgtt
     8701 tctcgccaac cataacttgc caccacgcgc cagaaagtgg gcgacagttg ttcaatctgt
     8761 acccgacgga cgttatggac atatggcgcg tcttcggtgc gcagagttaa cagaatcacc
     8821 cgctcatgca ataccttgtt atgtttaagg ttatgcatca gcgcaaaggg aatgacgttg
     8881 attgcacgtg acatatacac cgcggtcccg ggcacgcgaa cgggcggtga tttctccagc
     8941 gaagcaatca tcgcctccag agagttacca tgttcatgca tccgccgcag caaacggaaa
     9001 cgttcgctct tccaggtggt catcacgata aacatcaccg taccgaggct caatggcaac
     9061 cagccgccgg agagcagttt atcgaggtta gcggtgaaca atggaatatc gacacaaagg
     9121 aaagcaatca ggatcagcgc aacaaaatac ttattccagt gccagttctg acgtgccacg
     9181 gtagtcgaga gaatagacgt cagcaccatg gttccggtca ccgcaatccc gtacgccgcc
     9241 gccaggttgc tggagtgctc aaagctgaca atcacaatca cgaccgcgac atagagcatc
     9301 cagttcacaa agggaatata gatttgccct gactccattt cggaggtgtg aataatgcgc
     9361 atcggcgaca aatatcccag acgtaccgcc tgacgcgtca atgagaagac gccagagata
     9421 acagcctgcg aggcaattac cgtcgccagt gcggcgatga tcagcagcgg gatcagcgcc
     9481 cagtccggtg ccaacaggaa gaacgggttc ttaatcgctt ccgggttctt taacaacaac
     9541 gctccctgac cgaagtaatt aagggtcaag gaaggtaata cgacggtgaa ccacgccagg
     9601 cgaataggga acttaccaaa gtgccccata tcagcataca gcgcctcgac ccccgtaatc
     9661 gacagcacca ctgcccctaa tgcaataaaa gaaaccgttt tgtattcaag gaagaaatgc
     9721 accgcccaca tcggattcag cgcatgcagc acttccgggt tagcaataat gctacgtaac
     9781 cccagtcctg ccagaatcaa aaaccaggtc agcatgatcg gcgcaaacag cttaccgacc
     9841 atagccgtgc catgcttttg aatcataaat aataaagtga gaacgataat tgagagggga
     9901 actatccagg tatccagctg cggggcgacg atttccagac cttcaatggc ggacatcacc
     9961 gaaatagcgg gtgttatgac gacttcacca tagaaaaagc tgccgccgat tagccccata
    10021 atcaccagca ttgatgtggt tcgcgccgac gtattacgcc cggcaagcga catcaacgtc
    10081 aggatccccc cttcaccggc gttatctgcc cgcatcacga aggtgagata tttaatagaa
    10141 accacaaaga ttagcagcca gaagatcagc gataaaaagc caaacacggc atcgcgctca
    10201 acgccaaaac caaactggcc ggacaaacat tcacgaagtg tatataacgg gctggtacca
    10261 atatcgccgt agacaactcc aatcgccgcg agggtaatcg cgggcaatga ttgcttatta
    10321 tcagtgctca tagactagtc tttcgttgaa atatgaaatg tgtgcttagt cccttggccc
    10381 acaaaaaagc gcacagtatg cacgattaac ggcaaaatcg tactcctaaa tgcgggcaca
    10441 ttaacctggc tcaaagaaaa ataccccccc tttgagacta ttttagccac cagcaagaaa
    10501 cgtctatact cgcaatttac gcagaacttt tgacgaaagg acgccacttc attatggctc
    10561 accctcattt attagcggaa agaatttccc gcctgagcag ttcgttggaa aaggggcttt
    10621 atgaacgtag ccacgccatc cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt
    10681 tcctccttgg cccgccaggt attgccaaaa gtttgatcgc ccggcgctta aaattcgcct
    10741 ttcagaatgc ccgcgcgttt gaatatctga tgactcgctt ctccacgccg gaagaggttt
    10801 ttggtcccct ttctattcag gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg
    10861 gttacctgcc ggaagcagaa atcgtctttc tggatgagat ctggaaagcg ggtccggcaa
    10921 ttcttaatac cctgctcacc gccattaacg agcgccagtt ccgcaacggt gcacacgtag
    10981 aaaaaatccc gatgcgcctg ctggtggcgg cctccaacga gctgccggaa gcagacagca
    11041 gtctggaagc gttatatgac cgcatgctga ttcgtctgtg gttagataaa gtgcaggata
    11101 aagcgaattt ccgctccatg ctgaccagtc aacaggatga aaacgacaat ccggttcctg
    11161 cctccttgca ggtcacagat gaagaatatg aacgctggca gaaagagatt ggtgaaatta
    11221 cgctgcccga tcatgtattt gagctgattt ttatgctgcg ccagcaactg gataaattac
    11281 cggatgcgcc ttatgtctcg gatcgtcgct ggaaaaaagc gattcgatta ttgcaggcca
    11341 gcgccttttt tagcggtcgc agtgctgttg ccccggttga tctcattttg ctgaaagatt
    11401 gcctgtggta tgacgcgcaa agcctgaatt tgatacaaca acaaattgat gtattgatga
    11461 ccggtcatgc ctggcaacag caaggaatgt tgacccgcct gggcgcgatt gtgcaacgtc
    11521 acctgcaact ccagcagcaa caaagcgata aaacagcctt aacggtaatt cgtctgggcg
    11581 gcattttcag ccgtcgtcag cagtatcaac ttcctgttaa cgttactgct tccactctga
    11641 ctctgctgct gcaaaaaccg ttaaaactgc atgatatgga agtggttcat atctcctttg
    11701 agcgtaacgc gctggaacag tggctgagta aaggtggtga aattcgcggc aaactgaacg
    11761 gtatcggctt tgcccagaaa ttgaatctgg aagtggatag cgcccaacat cttgttgtac
    11821 gcgatgtaag tttacaaggc agtacgctgg cacttcccgg ttcatcggct gaaggtctgc
    11881 caggtgaaat aaaacaacaa ctggaagagc ttgaaagcga ctggcgcaag caacacgctt
    11941 tattcagcga acagcaaaaa tgtctgttta tccctggcga ctggttaggt cgcattgaag
    12001 ccagcctaca ggatgtcggc gcccagattc gccaggcaca acaatgctaa cgctggatac
    12061 gcttaatgtg atgctggccg tcagcgaaga gggattgatc gaagagatga tcatcgcgct
    12121 gctggcctca ccgcagctgg cggtcttctt tgaaaaattc ccacgactga aggcggcaat
    12181 cactgatgat gttccccgct ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt
    12241 cccgccggaa ctcaccgaag aggtgatgtg ctatcagcaa agccagctcc tctccacgcc
    12301 gcagtttatt gtgcagctac cacagatcct ggacttactg catcgtctga attctccatg
    12361 ggcagaacaa gcccgacagt tggttgatgc taacagtacg atcacttcag cgttacacac
    12421 gctttttctc cagcgctggc gtttaagtct gatcgtgcaa gcaacgacgc taaatcagca
    12481 gctattagaa gaagaacgcg aacaactgtt gagtgaagtt caggaacgca tgacgctgag
    12541 cgggcaactt gaaccgattc tcgcagataa caataccgca gctggtcgtc tgtgggatat
    12601 gagcgccggt cagcttaaac gcggcgacta tcagttgatt gtgaaatacg gtgaatttct
    12661 taacgaacag ccggaactga aacgcctggc agaacagttg gggcgttcac gggaagccaa
    12721 atcaataccg cgcaacgatg cgcagatgga aaccttccgc accatggtgc gcgaaccggc
    12781 gacggttcct gagcaggttg atggtctgca acaaagcgat gatattttac gtctcctgcc
    12841 gccagaactg gcgacactgg ggataacgga actggagtat gagttttacc gtcggctggt
    12901 ggaaaaacag ttgctcacct atcgcctgca cggtgagtcg tggcgtgaaa aagtgatcga
    12961 acgcccggtg gtacataaag attacgacga acagccgcgc ggaccgttta ttgtctgtgt
    13021 ggatacttcc ggctcaatgg gcggctttaa tgaacagtgt gcgaaagcgt tctgcatggc
    13081 cttgatgcgc attgctctcg ctgaaaaccg gcgctgctat attatgttat tttccaccga
    13141 gatcgtccgt tatgagcttt caggcccaca aggcatcgaa caagcaatcc gttttttaag
    13201 ccagcagttt cgtggcggca ccgatcttgc cagttgtttt cgcgccatta tggaacgctt
    13261 gcaaagcagg gaatggtttg atgccgatgc ggtggtgatt tctgatttta tcgcccagcg
    13321 gttgcctgac gacgtgacga gtaaagtgaa agagctacag cgcgtacatc agcatcgctt
    13381 tcatgccgtg gcgatgtctg cacatggcaa acccggcatc atgcgcattt tcgatcatat
    13441 ctggcgcttt gataccggga tgcgaagccg cctgctcaga cgctggcggc gataaattat
    13501 tacagcagag aagggacgct ctcgcgaaca gctgccggcc atacaccaca ctgaacctgg
    13561 ccgatatgtg gcagttgcag cagcagcatg gtcaaacgag actgcccgat accgccgccg
    13621 atggtctgcg gcatttcacc gcgcagcagc gcctgatgcc actccagctc caggcgatct
    13681 tcatcaccgg tcagcgccag ctgatgtttc aacgtatcgg catccacgcg gatccccatg
    13741 gaagaaagct caaacgcatc ttccagtacc gggttccata ccagaatatc gccgttcaga
    13801 cccgcatggc ccagctctga cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg
    13861 tgacgatgac catcactcaa cttgccgcca atcccgacga ggaataccgc gccaagatct
    13921 ttcgcaatcg cccgctcacg ccctttggca tcgagatccg gataacgaga cagtaactcc
    13981 tggctgtgta cgaagtggat ctgatccggc aggaacggtg ccaggccaaa ctcttcgcta
    14041 accgcagctt cggttgcttt aattcccgcc cagatcgcct ctaccgtgct tttcagagtc
    14101 gagaattgac gctcaccgtc gcccattacg cgttcccagt cccactggtc aacatagacc
    14161 gagtgcaacg gagaaagacg gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc
    14221 ccttcgcccg cgctgaagtc gtgttgccct aatgtctgac gtttccactt cgccagtgaa
    14281 tgaaccactt cgaactgggc atcaggcaga gctttcactt ttacctgcac cgctttttca
    14341 cagcccgaca agttatcctg cgtgccatcc cccacacggc taagaatcgg cgcctggact
    14401 tcgatcagcc ccagacgttc ttccagttga cgggaaaagt gagatttcac gaagctaatt
    14461 tgacgttgtt tggcaatgta agcggttttc attttttata ctcctgcgtc ctgttgctta
    14521 tgattaagca acaaaaaccg acacgcatgc aataatcatt caataaaaag cctaactcac
    14581 ttttgattca ttaaaaaaga aggctaaaat agaatgaatc atcaatccgc ataagaaaat
    14641 cctatggaaa attatctaat cgacaatctg gaccgtggca tcctggaagc attaatgggc
    14701 aatgcgcgca ccgcttacgc cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt
    14761 cacgttcgag tagagaaaat gaagcaggcg gggatcatta ccggggcgcg tattgatgtc
    14821 agcccgaagc agctcggtta tgacgtaggc tgctttatcg gcattatatt aaagagcgcc
    14881 aaagactatc cttccgcgct ggcgaaactg gaaagccttg atgaagtcac tgaagcctat
    14941 tacaccaccg gccactacag catctttata aaagtgatgt gccgttcgat cgacgctctc
    15001 cagcatgtac ttatcaacaa gatccaaaca attgatgaaa ttcagtccac cgagacattg
    15061 atcgtcctgc aaaacccgat catgcgtacc atcaagccct gatcggcttt tttaatccca
    15121 tacttttcca caggtagatc ccaacgcgtt cacagcgtac aatacgccac tcttaataaa
    15181 ggtggcggtt tatggcagat atcactctta tcagcggcag caccctcggc ggtgccgaat
    15241 atgtagcaga acacctggct gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc
    15301 acggtccgct gttggaagat ttatctgcct ctgggatctg gctggttatc agctcaaccc
    15361 acggtgccgg agatattccg gacaaccttt ctcctttcta tgaagcattg caggaacaga
    15421 agcccgatct ttctgcggtc cgctttggcg caatcggtat tggtagtcgt gaatatgaca
    15481 ccttttgtgg ggctatcgat aaactcgagg cagaactcaa aaattccggt gcaaaacaga
    15541 caggcgaaac actgaagatc aacattcttg atcacgacat tccggaagat ccggcagaag
    15601 aatggctggg atcgtggatt aatttactca aataagtata cagatcgtgc gatctactgt
    15661 ggataactct gtcaggaagc ttggatcaac cggtagttat ccaaagaaca accgttgttc
    15721 agtttttgag ttgtgtataa cccctcattc tgatcccagc ttatacggtc caggatcacc
    15781 gatcattcac agttaatgat cctttccagg ttgttgatct taaaagccgg atccttgtta
    15841 tccacagggc agtgcgatcc taataagaga tcacaataga acagatctct aaataaatag
    15901 atcttctttt taatacccag gatcccaggt ctttctcaag tcgacaaagt tgagtagaat
    15961 ccacggcccg ggcttcaatc cattttcata ccgcgttatg cgaggcaatc accatgtttt
    16021 atccggatcc ttttgacgtc atcatcattg gcgggggtca tgcaggcacc gaggccgcga
    16081 tggccgcggc gcgtatgggt caacagactc tgcttttgac acacaatatc gacactctgg
    16141 ggcagatgag ctgcaacccg gcgatcggcg gtattgggaa gggacatctg gtaaaagaag
    16201 tggatgcact cggcggtctg atggcgaaag cgatcgatca ggcgggtatc cagtttagga
    16261 tactaaacgc aagcaaggga ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc
    16321 tctaccgtca ggcggtacgt acggcgctgg agaaccaacc gaacctgatg atcttccagc
    16381 aggcggttga agatcttatt gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg
    16441 gactgaagtt ccgtgccaaa gccgtcgtgc ttaccgttgg gacgttcctc gacggtaaaa
    16501 ttcatatcgg tctggataac tacagcggtg gtcgtgctgg tgatccgccg tccattccgc
    16561 tctctcgccg tttgcgtgaa ctgccgctgc gcgttggtcg tctgaaaacc gggacaccgc
    16621 cgcgtattga tgcgcgtact atcgacttca gcgtgctggc gcaacagcat ggcgataacc
    16681 caatgccggt attctcgttt atgggtaatg cgtcccagca tccacagcag gtgccgtgtt
    16741 atatcaccca taccaacgag aaaacccatg atgtgatccg cagtaacctc gatcgtagcc
    16801 caatgtatgc aggggtgatc gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca
    16861 aagtcatgcg cttcgccgac agaaatcagc atcagatctt ccttgaaccg gaagggctga
    16921 cctcaaacga aatttatccg aacggtatct ccaccagcct gccgttcgat gtgcagatgc
    16981 aaatcgtccg ctccatgcag gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca
    17041 ttgagtatga cttcttcgat ccacgcgacc tgaaaccgac gctggagagc aagtttatcc
    17101 aggggctgtt ctttgctggt cagattaacg gcactaccgg ttacgaagaa gccgctgcgc
    17161 aaggtttgct ggccggtctt aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc
    17221 cggcgcgttc tcaggcgtat ctcggcgtac tggttgatga cctgtgcact ttaggaacca
    17281 aagaaccgta tcgtatgttt acctcgcgcg cagaatatcg tctgatgctg cgcgaagata
    17341 atgcggatct gcgtttgact gaaattggtc gtgaactggg cctggtggat gacgaacgtt
    17401 gggcgcgctt taacgagaaa cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga
    17461 cctgggtaac cccgtcggcg gaagctgcag ccgaagtgaa tgctcacctg actgcgccac
    17521 tttcccgtga agccagtggt gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat
    17581 taaccacgct gacgccgttt gcccctgcgt tgacagacga acaggcggcg gaacaggttg
    17641 agattcaggt taaatacgaa ggttatatcg cgcgccagca agatgagatc gaaaagcagc
    17701 tgcgtaacga gaacaccctg ctaccagcga cgctggatta ccgccaggta tccggtcttt
    17761 ctaacgaagt gatcgccaaa cttaacgatc acaaaccggc ctctatcggc caggcttcgc
    17821 gtatttctgg cgtcacgcct gcggccatct ccattctgct ggtgtggctg aaaaaacagg
    17881 gtatgctgcg tcgtagcgca taacgcatta aaaatgcctg gtaagcaccc gcttaccggg
    17941 caacgcatca agaacaggta atcaccgtgc tcaacaaact ctccttactg ctgaaagacg
    18001 caggtatttc gcttaccgat caccagaaaa accagcttat tgcctatgtg aatatgctgc
    18061 ataaatggaa taaagcgtac aacctgactt cggtccgcga tcctaatgag atgctggtac
    18121 gccatattct cgatagcatt gtggtggcac cgtatctgca aggtgaacgg tttatcgatg
    18181 tcggcaccgg tccaggactg cctggcattc cactctctat cgtgcgtcct gaagcccatt
    18241 tcactctgtt ggatagcctt ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc
    18301 ttaaactgga gaatattgag ccagtacaga gcagggtaga agagtttcct tcagagccgc
    18361 catttgatgg cgtaattagc cgcgcttttg cctctctgaa cgatatggtg agctggtgtc
    18421 accatcttcc tggtgagcaa ggccgtttct acgcgctgaa aggccaaatg ccggaagatg
    18481 aaatcgcttt gttgcccgaa gaatatcagg tcgaatcagt ggttaaactt cacgttccgg
    18541 ccctggatgg cgaacgtcat ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa
    18601 aaaaatcata aaaaattgac cggttagact gttaacaaca accaggtttt ctactgatat
    18661 aactggttac atttaacgcc acgttcactc ttttgcatca acaagataac gtggcttttt
    18721 ttggtaagca gaaaataagt cataagtgaa aatatcagtc tgctaaaaat cggcgctaag
    18781 aatcatcatt ggctgttaaa acattattaa aaatgtcaat gggtggtttt tgttgtgtaa
    18841 atgtcattta ttaaaacagt atctgttttt agactgaaat atcataaact tgcaaaggca
    18901 tcatttgcca agtaaataaa tatgctgtgc gcgaacatgc tcaatatgtg atctgaagca
    18961 cgctttatca ccagtgttta cgcgttattt acagtttttc atgatcgaac agggtttgca
    19021 gaaaagtcgc aattgtatgc actggaaaaa tatttaaaca tttattcacc ttttggctac
    19081 ttattgtttg aaatcacggg ggcgcaccgt ataatttgac cgctttttga tgcttgactc
    19141 taagccttaa agaaagtttt atacgacacg cggcatacct cgaagggagc aggagtgaaa
    19201 aacgtgatgt ctgtgtcgct cgtgagtcga aacgttgctc ggaagcttct gctcgttcag
    19261 ttactggtgg tgatagcaag tggattgctg ttcagcctca aagacccctt ctggggcgtc
    19321 tctgcaataa gcgggggcct ggcagttttt ctgcctaacg ttttgtttat gatatttgcc
    19381 tggcgtcacc aggcgcatac accagcgaaa ggccgggtgg cctggacatt cgcatttggc
    19441 gaagctttca aagttctggc gatgttggtg ttactggtgg tggcgttggc ggttttaaag
    19501 gcggtattct tgccgctgat cgttacgtgg gttttggtgc tggtggttca gatactggca
    19561 ccggctgtaa ttaacaacaa agggtaaaag gcatcatggc ttcagaaaat atgacgccgc
    19621 aggattacat aggacaccac ctgaacaacc ttcagctgga cctgcgtaca ttctcgctgg
    19681 tggatccaca taacccccca gccaccttct ggacaatcaa tattgactcc atgttcttct
    19741 cggtggtgct gggtctgttg ttcctggttt tattccgtag cgtagccaaa aaggcgacca
    19801 gcggtgtgcc aggtaagttt cagaccgcga ttgagctggt gatcggcttt gttaatggta
    19861 gcgtgaaaga catgtaccat ggcaaaagca agctgattgc tccgctggcc ctgacgatct
    19921 tcgtctgggt attcctgatg aacttgatgg atttactgcc tatcgacctg ctgccgtaca
    19981 ttgctgaaca tgtactgggt ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg
    20041 taacgctgtc tatggcactg ggtgtattta tcctgattct gttctacagc atcaaaatga
    20101 aaggcatcgg cggcttcacg aaagagttga cgctgcagcc gttcaatcac tgggcgttca
    20161 ttcctgtcaa cttaatcctt gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt
    20221 tgcgactgtt cggtaacatg tatgccggtg agctgatttt cattctgatt gctggtctgt
    20281 tgccgtggtg gtcacagtgg attctgaatg tgccgtgggc cattttccac atcctgatca
    20341 ttacgctgca agccttcatc ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg
    20401 aagaacatta atttaccaac actactacgt tttaactgaa acaaactgga gactgtcatg
    20461 gaaaacctga atatggatct gctgtacatg gctgccgctg tgatgatggg tctggcggca
    20521 atcggtgctg cgatcggtat cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt
    20581 caacctgatc tgattcctct gctgcgtact cagttcttta tcgttatggg tctggtggat
    20641 gctatcccga tgatcgctgt aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa
    20701 gcgttgcttt tatttaaaga gcaatatcag aacgttaact aaatagaggc attgtgctgt
    20761 gaatcttaac gcaacaatcc tcggccaggc catcgcgttt gtcctgttcg ttctgttctg
    20821 catgaagtac gtatggccgc cattaatggc agccatcgaa aaacgtcaaa aagaaattgc
    20881 tgacggcctt gcttccgcag aacgagcaca taaggacctt gaccttgcaa aggccagcgc
    20941 gaccgaccag ctgaaaaaag cgaaagcgga agcccaggta atcatcgagc aggctaacaa
    21001 acgccgctcg cagattctgg acgaagcgaa agctgaggca gaacaggaac gtactaaaat
    21061 cgtggcccag gcgcaggcgg aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg
    21121 taagcaagtt gctatcctgg ctgttgctgg cgccgagaag atcatcgaac gttccgtgga
    21181 tgaagctgct aacagcgaca tcgtggataa acttgtcgct gaactgtaag gagggagggg
    21241 ctgatgtctg aatttattac ggtagctcgc ccctacgcca aagcagcttt tgactttgcc
    21301 gtcgaacacc aaagtgtaga acgctggcag gacatgctgg cgtttgccgc cgaggtaacc
    21361 aaaaacgaac aaatggcaga gcttctctct ggcgcgcttg cgccagaaac gctcgccgag
    21421 tcgtttatcg cagtttgtgg tgagcaactg gacgaaaacg gtcagaacct gattcgggtt
    21481 atggctgaaa atggtcgtct taacgcgctc ccggatgttc tggagcagtt tattcacctg
    21541 cgtgccgtga gtgaggctac cgctgaggta gacgtcattt ccgctgccgc actgagtgaa
    21601 caacagctcg cgaaaatttc tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg
    21661 aattgcaaaa tcgataagtc tgtaatggca ggcgttatca tccgagcggg tgatatggtc
    21721 attgatggca gcgtacgcgg tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg
    21781 gactggagca tgcaactgaa ttccaccgaa atcagcgaac tgatcaagca gcgcattgct
    21841 cagttcaatg ttgtgagtga agctcacaac gaaggtacta ttgtttctgt aagtgacggt
    21901 gttatccgca ttcacggcct ggccgattgt atgcagggtg aaatgatctc cctgccgggt
    21961 aaccgttacg ctatcgcact gaacctcgag cgcgactctg taggtgcggt tgttatgggt
    22021 ccgtacgctg accttgccga aggcatgaaa gttaagtgta ctggccgtat cctggaagtt
    22081 ccggttggcc gtggcctgct gggccgtgtg gttaacactc tgggtgcacc aatcgacggt
    22141 aaaggtccgc tggatcacga cggcttctct gctgtagaag caatcgctcc gggcgttatc
    22201 gaacgtcagt ccgtagatca gccggtacag accggttata aagccgttga ctccatgatc
    22261 ccaatcggtc gtggtcagcg tgaattgatc atcggtgacc gtcagaccgg taaaaccgca
    22321 ctggctatcg atgccatcat caaccagcgc gattccggta tcaaatgtat ctatgtcgct
    22381 atcggccaga aagcgtccac catttctaac gtggtacgta aactggaaga gcacggcgca
    22441 ctggctaaca ccatcgttgt ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg
    22501 gcaccgtacg ctggttgtgc aatgggcgaa tacttccgtg accgcggtga agatgcgctg
    22561 atcatttacg atgacctgtc taaacaggct gttgcttacc gtcagatctc cctgctgctc
    22621 cgtcgtccgc caggacgtga agcattcccg ggcgacgtat tctacctcca ctctcgtctg
    22681 ctggagcgtg ctgcgcgtgt taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg
    22741 aaagggaaaa ccggttctct gactgcgctg ccgattatcg aaactcaggc gggtgacgtt
    22801 tctgcgttcg ttccaaccaa cgtaatctcc attaccgatg gtcagatctt cctggaaacc
    22861 aacctgttca acgccggtat tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt
    22921 ggtggtgcag cacagaccaa gatcatgaaa aaactgtccg gtggtatccg taccgctctg
    22981 gcacagtatc gtgaactggc agcgttctct cagtttgcat ccgaccttga cgatgcaaca
    23041 cgtaagcagc ttgaccacgg tcagaaagtg accgaactgc tgaagcagaa acagtatgcg
    23101 ccgatgtctg ttgcgcaaca gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg
    23161 gatgtagaac tgtcgaaaat cggcagcttc gaagccgctc tgctggctta cgtcgaccgt
    23221 gatcacgctc cgttgatgca agagatcaac cagaccggtg gctacaacga cgaaatcgaa
    23281 ggcaagctga aaggcatcct cgattccttc aaagcaaccc aatcctggta acgtctggcg
    23341 gcttgcctta gggcaggccg caaggcattg aggagaagct catggccggc gcaaaagaga
    23401 tacgtagtaa gatcgcaagc gtccagaaca cgcaaaagat cactaaagcg atggagatgg
    23461 tcgccgcttc caaaatgcgt aaatcgcagg atcgcatggc ggccagccgt ccttatgcag
    23521 aaaccatgcg caaagtgatt ggtcaccttg cacacggtaa tctggaatat aagcaccctt
    23581 acctggaaga ccgcgacgtt aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt
    23641 tgtgcggtgg tttgaacatt aacctgttca aaaaactgct ggcggaaatg aagacctgga
    23701 ccgacaaagg cgttcaatgc gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca
    23761 actccgtggg cggcaatgtt gttgcccagg tcaccggcat gggggataac ccttccctgt
    23821 ccgaactgat cggtccggta aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca
    23881 agctttacat tgtcagcaac aaatttatta acaccatgtc tcaggttccg accatcagcc
    23941 agctgctgcc gttaccggca tcagatgatg atgatctgaa acataaatcc tgggattacc
    24001 tgtacgaacc cgatccgaag gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc
    24061 aggtttatca gggcgtggtt gaaaacctgg ccagcgagca ggccgcccgt atggtggcga
    24121 tgaaagccgc gaccgacaat ggcggcagcc tgattaaaga gctgcagttg gtatacaaca
    24181 aagctcgtca ggccagcatt actcaggaac tcaccgagat cgtctcgggg gccgccgcgg
    24241 tttaaacagg ttatttcgta gaggatttaa gatggctact ggaaagattg tccaggtaat
    24301 cggcgccgta gttgacgtcg aattccctca ggatgccgta ccgcgcgtgt acgatgctct
    24361 tgaggtgcaa aatggtaatg agcgtctggt gctggaagtt cagcagcagc tcggcggcgg
    24421 tatcgtacgt accatcgcaa tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa
    24481 agacctcgaa cacccgatcg aagtcccggt aggtaaagcg actctgggcc gtatcatgaa
    24541 cgtactgggt gaaccggtcg acatgaaagg cgagatcggt gaagaagagc gttgggcgat
    24601 tcaccgcgca gcaccttcct acgaagagct gtcaaactct caggaactgc tggaaaccgg
    24661 tatcaaagtt atcgacctga tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg
    24721 tggtgcgggt gtaggtaaaa ccgtaaacat gatggagctt attcgtaaca tcgcgatcga
    24781 gcactccggt tactctgtgt ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt
    24841 ctaccacgaa atgaccgact ccaacgttat cgacaaagta tccctggtgt atggccagat
    24901 gaacgagccg ccgggaaacc gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa
    24961 attccgtgac gaaggtcgtg acgttctgct gttcgttgac aacatctatc gttacaccct
    25021 ggccggtacg gaagtatccg cactgctggg ccgtatgcct tcagcggtag gttatcagcc
    25081 gaccctggcg gaagagatgg gcgttctgca ggaacgtatc acctccacca aaaccggttc
    25141 tatcacctcc gtacaggcgg tatacgtacc tgcggatgac ttgactgacc cgtctccggc
    25201 aaccaccttt gcgcaccttg acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg
    25261 tatctacccg gccgttgacc cgctggactc caccagccgt cagctggacc cgctggtggt
    25321 tggtcaggaa cactacgaca ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga
    25381 actgaaagac attatcgcca tcctgggtat ggatgaactg tctgaagaag acaaactggt
    25441 ggtagcgcgt gctcgtaaga tccagcgctt cctgtcccag ccgttcttcg tggcagaagt
    25501 attcaccggt tctccgggta aatacgtctc cctgaaagac accatccgtg gctttaaagg
    25561 catcatggaa ggcgaatacg atcacctgcc ggagcaggcg ttctacatgg tcggttccat
    25621 cgaagaagct gtggaaaaag ccaaaaaact ttaacgcctt aatcggaggg tgatatggca
    25681 atgacttacc acctggacgt cgtcagtgca gagcaacaaa tgttctctgg tctggtcgag
    25741 aaaatccagg taacgggtag cgaaggtgaa ctggggatct atcctggcca cgcaccgctg
    25801 ctcaccgcca ttaagcctgg tatgattcgc atcgtgaaac agcacggtca cgaagagttt
    25861 atctatctgt ctggcggcat tcttgaagtg cagcctggca acgtgaccgt tctggccgac
    25921 accgcaattc gcggccagga tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct
    25981 gaagagcaca ttagcagctc tcacggcgac gtagattacg ctcaggcgtc tgcggaactg
    26041 gccaaagcga tcgcgcagct gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg
    26101 cttgaaaagc acaaaagcca gtctggaaac aggctggctt tttttacgcg tgacccgtcc
    26161 tgaagaacgt tcacatagat cctgctgata taaaaccccc tgttttcccg tttattcatt
    26221 gatcgaaata agagcaaaaa catccacctg acgcttaaat gaaggtactg ccttaatttt
    26281 ctgcagacaa aaggtgtgac gatggtcgaa aatggcgctt tcgtcagcgg ggataatccg
    26341 ttattgaaca atttatcctc tgtccatttc acgatgaaaa aaatgtagtt ttttcaaggt
    26401 gaagcggttt aaattcgttc tcaaattaca gtcaggacgc gtatgttgaa taatgctatg
    26461 agcgtagtga tccttgccgc aggcaaaggc acgcgcatgt attccgatct tccgaaagtg
    26521 ctgcataccc ttgccgggaa agcgatggtt cagcatgtca ttgatgctgc gaatgaatta
    26581 ggcgcagcgc acgttcacct ggtttacggt cacggcggcg atctgcttaa acaggcgctg
    26641 aaagatgaca acctgaactg ggtgcttcag gcagaacagc tgggtacagg tcatgcgatg
    26701 cagcaggccg cacctttctt tgccgatgat gaagacattt taatgctcta cggcgacgtg
    26761 ccgctgatct ctgtcgaaac actccagcgt ctgcgtgatg ctaaaccgca gggtggcatt
    26821 ggtctgctga cggtaaaact ggatgatccg accggttatg gacgtatcac ccgtgaaaac
    26881 ggcaaagtta ccggcattgt tgagcacaaa gacgccaccg acgagcagcg tcagattcag
    26941 gagatcaaca ccggcattct gattgccaac ggcgcagata tgaaacgctg gctggcgaag
    27001 ctgaccaaca ataacgctca gggtgaatac tacatcaccg acattattgc gctggcgtat
    27061 caggaagggc gtgaaatcgt cgccgttcat ccgcaacgtt taagcgaagt agaaggcgtg
    27121 aataaccgcc tgcaactctc ccgactggag cgcgtttacc agtctgaaca ggctgaaaaa
    27181 ctgctgttag caggcgttat gctgcgcgat ccggcgcgtt ttgatctgcg cggtacgctt
    27241 actcacgggc gcgatgttga aattgatact aacgttatca tcgagggcaa cgtgactctc
    27301 ggtcatcgcg tgaaaatcgg caccggttgc gtgattaaaa acagcgtgat tggcgatgat
    27361 tgcgaaatca gtccgtatac cgttgtggaa gatgcgaatc tggcagcggc ctgtaccatt
    27421 ggcccgtttg cccgtctgcg tcctggtgct gagttgctgg aaggtgcaca cgtcggtaac
    27481 ttcgttgaga tgaaaaaagc gcgtctgggt aaaggctcga aagccgggca tctgacttat
    27541 ctgggcgatg cggaaattgg cgataacgtt aacatcggcg cgggaaccat tacctgcaac
    27601 tacgacggtg cgaataaatt taagaccatt atcggcgacg atgtgtttgt tggttccgat
    27661 actcagctgg tggccccggt aacggtaggc aaaggcgcaa ccattgctgc gggtacaact
    27721 gtgacgcgta atgtcggcga aaatgctctg gcaatcagcc gtgtgccgca gtcgcaaaaa
    27781 gaaggctggc gtcgtccggt aaagaaaaag taattctggc tgggaaatcc ggtcatatgg
    27841 gatgaggaga taacataatc tcccgcccac aagcagtaac tataaaaata accccactct
    27901 ctacaaggct cggggcgccc gaaaaaacgg gcatacaggt tgaccgacaa cgatataaat
    27961 cggaatcaaa aactatgtgt ggaattgttg gcgcgatcgc gcaacgtgat gtagcagaaa
    28021 tccttcttga aggtttacgt cgtctggaat accgcggata tgactctgcc ggtctggccg
    28081 ttgttgatgc agaaggtcat atgacccgcc tgcgtcgcct cggtaaagta cagatgctgg
    28141 ctcaggcagc ggaagaacat cctctgcatg gcggcaccgg tattgctcat actcgctggg
    28201 cgacccacgg tgaaccttca gaagctaacg cgcatccgca tgtttctgaa cacattgtgg
    28261 tggtgcataa cggcatcatc gaaaaccatg aaccgctgcg tgaagagtta aaagcgcgtg
    28321 gctatacctt cgtttctgaa accgacaccg aagtgattgc acatttggtg aactgggagc
    28381 tgaaacaagg cggtactctg cgtgaggcag ttctgcgtgc tattccgcaa ctgcgtggtg
    28441 cgtacggtac agtgatcatg gactcccgtc acccggatac cctgctggcg gcacgttctg
    28501 gtagcccgct ggtgattggt ctgggcatgg gcgaaaactt tatcgcttct gaccagctgg
    28561 cgctgttgcc agtgactcgt cgctttatct tccttgaaga gggcgatatt gcggaaatca
    28621 ctcgccgttc ggtaaacatt ttcgataaaa ctggcgcgga agtaaaacgt caggatattg
    28681 aatccaatct gcaatatgac gcgggcgata aaggtattta ccgtcactac atgcagaaag
    28741 agatctacga acaaccgaac gcgatcaaaa acacccttac cgggcgcatc agccacggtc
    28801 aggttgattt aagcgagctg ggaccgaacg ccgacgaact gctgtcgaag gttgagcata
    28861 ttcagatcct cgcctgtggt acttcttata actccggtat ggtttcccgc tactggtttg
    28921 aatcgctggc aggtattccg tgcgacgtcg aaatcgcctc tgaattccgc tatcgcaaat
    28981 ctgccgtgcg tcgtaacagc ctgatgatca ccttgtcaca gtctggcgaa accgcggata
    29041 ccctggcggg cctgcgtctg tcgaaagagc tgggttacct tggttcgctg gcaatctgta
    29101 acgttccggg ttcttctctg gtgcgcgaat ccgatctggc gctgatgacc aacgccggta
    29161 cggaaatcgg cgtggcatcc actaaagcat tcaccactca gttaactgtg ctgttgatgc
    29221 tggtggcgaa gctgtctcgc ctgaaaggtc tggatgcctc cattgaacat gacattgtgc
    29281 atggtctgca ggcgctgccg agccgtattg agcagatgct gtctcaggac aaacgcattg
    29341 aagcgctggc agaagatttc tctgacaaac atcacgcgct gttcctgggc cgtggcgatc
    29401 agtacccaat cgcgctggaa ggtgcattga agctgaaaga gatctcttac attcacgctg
    29461 aagcctacgc tgcgggcgaa ctgaaacacg gcccgctggc gctgattgac gccgatatgc
    29521 cggttatcgt cgttgcgccg aacaacgagt tgttggaaaa actgaaatct aacattgaag
    29581 aagttcgcgc gcgtggcggt cagttgtatg tcttcgccga tcaggatgcg ggttttgtga
    29641 gcagtgacaa catgcacatc attgagatgc cgcatgtgga agaggtgatt gcaccaatct
    29701 tctacaccgt tccgctgcag ctactggctt accacgttgc gctgatcaaa ggcaccgacg
    29761 ttgaccagcc gcgtaacctg gcgaaatcgg ttacggttga gtaatatctc tcgttgatag
    29821 aaaagggctg ccgaccggcg gccctttttt ataagtgata tcaataattc cattattcat
    29881 atgcccatt
//
LOCUS       DAZGTJ010000063        29707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000063 DAZGTJ010000000
VERSION     DAZGTJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.063"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..728)
                     /locus_tag="ACHK8E_003322"
     CDS             complement(<1..728)
                     /locus_tag="ACHK8E_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0345546.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITELIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAI"
     gene            complement(986..2239)
                     /gene="amiC"
                     /locus_tag="ACHK8E_003323"
     CDS             complement(986..2239)
                     /gene="amiC"
                     /locus_tag="ACHK8E_003323"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP0345547.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2471..3802
                     /gene="argA"
                     /locus_tag="ACHK8E_003324"
     CDS             2471..3802
                     /gene="argA"
                     /locus_tag="ACHK8E_003324"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP0345548.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(4040..5866)
                     /gene="recD"
                     /locus_tag="ACHK8E_003325"
     CDS             complement(4040..5866)
                     /gene="recD"
                     /locus_tag="ACHK8E_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP0345549.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5866..9408)
                     /gene="recB"
                     /locus_tag="ACHK8E_003326"
     CDS             complement(5866..9408)
                     /gene="recB"
                     /locus_tag="ACHK8E_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP0345550.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9401..12289)
                     /gene="ptrA"
                     /locus_tag="ACHK8E_003327"
     CDS             complement(9401..12289)
                     /gene="ptrA"
                     /locus_tag="ACHK8E_003327"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP0345551.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12465..15833)
                     /gene="recC"
                     /locus_tag="ACHK8E_003328"
     CDS             complement(12465..15833)
                     /gene="recC"
                     /locus_tag="ACHK8E_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP0345552.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15846..16169)
                     /gene="ppdC"
                     /locus_tag="ACHK8E_003329"
     CDS             complement(15846..16169)
                     /gene="ppdC"
                     /locus_tag="ACHK8E_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP0345553.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16154..16561)
                     /gene="ygdB"
                     /locus_tag="ACHK8E_003330"
     CDS             complement(16154..16561)
                     /gene="ygdB"
                     /locus_tag="ACHK8E_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP0345554.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16558..17121)
                     /gene="ppdB"
                     /locus_tag="ACHK8E_003331"
     CDS             complement(16558..17121)
                     /gene="ppdB"
                     /locus_tag="ACHK8E_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0345555.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(17112..17582)
                     /gene="ppdA"
                     /locus_tag="ACHK8E_003332"
     CDS             complement(17112..17582)
                     /gene="ppdA"
                     /locus_tag="ACHK8E_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0345556.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17767..18561)
                     /gene="thyA"
                     /locus_tag="ACHK8E_003333"
     CDS             complement(17767..18561)
                     /gene="thyA"
                     /locus_tag="ACHK8E_003333"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0345557.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18568..19443)
                     /gene="lgt"
                     /locus_tag="ACHK8E_003334"
     CDS             complement(18568..19443)
                     /gene="lgt"
                     /locus_tag="ACHK8E_003334"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP0345558.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19594..21840)
                     /gene="ptsP"
                     /locus_tag="ACHK8E_003335"
     CDS             complement(19594..21840)
                     /gene="ptsP"
                     /locus_tag="ACHK8E_003335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0345559.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21853..22383)
                     /gene="rppH"
                     /locus_tag="ACHK8E_003336"
     CDS             complement(21853..22383)
                     /gene="rppH"
                     /locus_tag="ACHK8E_003336"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP0345560.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22736..22882)
                     /gene="ygdT"
                     /locus_tag="ACHK8E_003337"
     CDS             complement(22736..22882)
                     /gene="ygdT"
                     /locus_tag="ACHK8E_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP0345561.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            23068..23757
                     /gene="mutH"
                     /locus_tag="ACHK8E_003338"
     CDS             23068..23757
                     /gene="mutH"
                     /locus_tag="ACHK8E_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP0345562.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23826..24539
                     /gene="ygdQ"
                     /locus_tag="ACHK8E_003339"
     CDS             23826..24539
                     /gene="ygdQ"
                     /locus_tag="ACHK8E_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0345563.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24677..24895
                     /gene="ygdR"
                     /locus_tag="ACHK8E_003340"
     CDS             24677..24895
                     /gene="ygdR"
                     /locus_tag="ACHK8E_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP0345564.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            25003..26043
                     /gene="tas"
                     /locus_tag="ACHK8E_003341"
     CDS             25003..26043
                     /gene="tas"
                     /locus_tag="ACHK8E_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP0345565.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(26075..27268)
                     /gene="lplT"
                     /locus_tag="ACHK8E_003342"
     CDS             complement(26075..27268)
                     /gene="lplT"
                     /locus_tag="ACHK8E_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP0345566.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(27261..29420)
                     /gene="aas"
                     /locus_tag="ACHK8E_003343"
     CDS             complement(27261..29420)
                     /gene="aas"
                     /locus_tag="ACHK8E_003343"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP0345567.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
BASE COUNT         7296 a         8065 c         7351 g         6995 t
ORIGIN      
        1 tgaattgcgc gctgtaacag gatgtccttt tcaagaaact gttcatagat atcaccaata
       61 tctttctgtt ttattttttc attgcataac acgcgacttg aaaaatattt aaggtgcatc
      121 ataaatctgg aatagttaaa tgaacttctg tccacctttc tgttataaac aacttcaata
      181 agctccgtga ttatttgcga caactcaata atccgtactg attcataatc aactgaattt
      241 tcataattat ttaaaatatg caatgctata aaaccaatct catcatcagg aagttgaacg
      301 ctgaattgct cattaatgat agttaatgca tcgcgactta tcgcatactc tgctttgtac
      361 aacgctttga tttcatcaag gaaaacatta ttaatttgta tacctgaaac tactcgggat
      421 attgcattcg aaatgtgatc cgcaagaatc aatagtagcg atggattgag atttttttcg
      481 agatttttct ccgcatattg cactattttt tcggcaacaa aaatatactc aacatccaca
      541 tgttcaatca atttgtagag tttatttttt tgttcgtttc tgacataaaa aatccggtta
      601 gccggatgtt cagggaccga cattccatat tttttgttgt aaccgacgcc aggtccagaa
      661 atgataacct cctgtccatt catcgatgcc tgtacacaat tattgttcat gactttttcg
      721 atgatcattc catgactcca aaaaaaaagg caaggcacgt cagaagtaat aattacttca
      781 aacattgcct tgcctgattt aacagttaca agatgattaa ctaatgcgta tacattacac
      841 ggaattgtgc ttaattaaag agatggtcgg cttatttttc aagccgacat tcaaatattc
      901 gacatcagtc acaaaaccag catctaatat tcatgaaaat gttatctatt caacgaaata
      961 taaattcatt gaataaataa aaacatcatc cccttctcgc cagcgtcgcc ccatcggcaa
     1021 aatacgcttt aatccccgca agaatagact ccgcaacttc ctgctggaaa gtcgccgttt
     1081 tcagtttacg ttcttcctca acgttactga taaacgccgt ttcgaccaga atggagggaa
     1141 tatctggtgc ctttagtacg gcaaacccgg cctgttcaac ttgattttta tgcagcttgt
     1201 tgattttacc gagcttattc agcaccgctt taccaaactt caggctgtcg gcaatggtca
     1261 gcgactgcac catatcgaac atggtgtggt cgacatagcg gtcaccgctt ttgctcacgc
     1321 caccaatcaa gtccgaggcg ttctgggttt gtgccagata ttttgccgca gtactggttg
     1381 cacctttggt tgagagcgca aacacagagg aaccgctcgg ctgacgactg gtaaaggcgt
     1441 cggcatggat agagacaaac aggtcagcac gctgtttctg ggcttttgct acgcgcactt
     1501 gcaacggaat gaagatgtct tcattgcgcg tcatgtacac cttcatattg ccctctttct
     1561 cgatcagaga gcgcagacgg cgagctattt gcaataccac gtctttttcg cgcgttttgt
     1621 atttccccac cgcaccggag tcttcgccac cgtggccagg gtcaagcata atgacaatag
     1681 gacgatcgcg ccctgcttta cccggttgtg gaccactttg tgctggcggc acctgctttt
     1741 cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag cgggtcctgc atatcctgtg
     1801 cattggccgg atagaggtcc atcaccagac gctctttaaa cccggcgacc ggcgcaaggg
     1861 caaacagctg cggttttacg ttttgcttta attcaaaaac catacgtacg gtttgcgggt
     1921 caaattgccc gacgcgcgcc gacttgatga acgggtcgtc agcgcggatt tgcgcagcca
     1981 tccccttgag taccgagttc aggtttacat cttcgatatc caccaccacg cgttcaggat
     2041 tactcaacgc gaactgctta tatttcagct gacgatttga ttctaccgtc acgcgggtgt
     2101 agctggacgc aggccagacg cgcaccgcca cgacctggct gaccgcagcc aggctgacct
     2161 gacttacgct caatagccac atggcacccg cgccttgcag taaacgacga cggctgattg
     2221 cagtgttgga tcctgacatg cctctcccga gcaaaagaaa tctaagctgt gtaacaagta
     2281 aacgaccaat ttgaccggaa actttagcga aagacgcata atctgtcatc tataaaacgg
     2341 taaacattct ttttatattc acggtattac tgataaaaaa ctcgctcccg cataaaattt
     2401 acacttgcac cctgcgaaaa aacagaataa aaatacaata atttcgaata atcatgcaaa
     2461 gaggtgtacc gtggtaaagg aacgtaaaac cgagctggtc gagggattcc gccattcggt
     2521 tccctatatc aatacccacc ggggaaaaac atttgtcatc atgctcggcg gtgaagccat
     2581 tgagcatgag aatttctcca gtatcgttaa tgatatcggg ttgttgcaca gcctcggcat
     2641 ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac gcaaatctgg ctgcgcatca
     2701 ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac gccaaaacac tggaactggt
     2761 gaagcaggct gcgggaacat tgcaactgga tattactgct cgcctgtcga tgagtctcaa
     2821 taacacgccg ctacagggcg cgcatatcaa cgtcgtcagt ggcaatttta ttatcgccca
     2881 gccgctgggc gtcgatgacg gcgtggatta ctgccatagc gggcgtatcc ggcggattga
     2941 tgaagacgcg atccatcgtc aactggacag cggtgcaata gtgctaatgg ggccggtcgc
     3001 tgtttcagtc actggcgaga gctttaatct gacctcggaa gagattgcca ctcaactggc
     3061 catcaaactg aaagctgaaa agatgattgg tttttgctct tcccagggcg tcactaatga
     3121 cgacggtgat attgtctccg aacttttccc taacgaagcg caagcacggg tagaagccca
     3181 ggaagagaaa ggtgattaca actccggtac ggtgcgcttt ttgcgtggcg cagtgaaagc
     3241 ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat caggaagatg gcgcgctatt
     3301 gcaagagttg ttctcacgcg acggtatcgg tacgcagatt gtgatggaaa gcgccgagca
     3361 gattcgtcgc gcaacaatca acgatattgg cggtattctg gagttgattc gcccgctgga
     3421 gcagcaaggt attctggtac gccgttctcg cgagcagcta gagatggaaa tcgacaaatt
     3481 caccattatt cagcgcgata acacgactat tgcctgcgcc gcgctctatc cgttcccgga
     3541 agagaagatt ggggaaatgg cctgtgtggc agttcacccg gattaccgca gctcatcaag
     3601 gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag cagagcggct taagcaaatt
     3661 gtttgtgctg accacgcgca gtattcactg gttccaggaa cgtggattta ccccggtgga
     3721 tattgattta ctccccgaga gcaaaaagca gttgtacaac taccagcgta aatccaaagt
     3781 gttgatggcg gatttggggt aagtggtgga aatcgccaga tgcgacatgc gcaatgcctg
     3841 gaggcatgat aaacacgcta gcatcgcatc aggtattgac gctcatcgtc ggatgcggcg
     3901 tgaacgcctt atccgaccta catcaaatca aaggtgttga tggcggattt agggtaagcg
     3961 acggaaatcg ccagatgcgc atatgcgcaa tgcccggagg catgacagga cgcgcatgcg
     4021 tcgcatcagg caatcacgtt tattcccgcg aactaaacaa tgccgccaga ccactgcgcc
     4081 gctcagtgcg cgtggcaatt gccgcactta aaatgcgctc atcggcatac agcgacagac
     4141 ggcgacgcgc gcgggtcact gcggtgtaaa ccagctctcg cgttactacc ggcgtgcgtt
     4201 gactcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca
     4261 tcgcccacgt cgtttcgtgc tctggcaggc gactcggttg cacagactta atattgccgt
     4321 ccggcatcgc aaaccaaacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc
     4381 cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc
     4441 aacgagagtg cggattacga tgaattttgc gcttctgttg cataaactgc tcaattcgct
     4501 cattcagtcc agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat
     4561 tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgcc
     4621 cgtaacccgc aagagcctcc tcaagcatcg caatatagtc ttcgccgctc tgtaagagcc
     4681 gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac
     4741 ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt
     4801 gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccagttcct gacggaacgt
     4861 gagttcccgt caggcggctc aactgcccgg cacgctcggc ggtaaagccc gcgttggcat
     4921 aagcgcagat atcgcccagc acagctccag cctcaactga ggccagttga tcgcgatcac
     4981 cgaggaagat cactcgcgca tgatcgggca acgcatcgat caggcgcgac atcatgggga
     5041 gatcgatcat tgacgcttca tccaccacca gcacatcaag atgcagcggg ttaccggcat
     5101 gatgacgtaa acgctggcta cccggctgcg cacccagcaa tcggtgcaaa gtgctggcat
     5161 cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctggcgcaaa gccttgccga
     5221 gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc
     5281 gttcgccgtc tgccatttga attaacgctg ccagcaactt cgctacggtg gtcgttttac
     5341 cggtgccagg gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgccgcca
     5401 ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta
     5461 gagcttcatc aacctcaatg gcatgattca cttcgttgaa aaagcgtgcc actgtgcgct
     5521 cgttacacca catgcgattc aaataaagac gatcaccaca gaggatcatc ggcgttggtt
     5581 catctccccg gctgaccgct tgtgaagcca gcaagcattc ttcccaattt tgtaactcac
     5641 cgatttcgct gacgcaggtt gctaacagcg gatgtgacgc ctcgttattt tccagtcgtg
     5701 agagcggtaa acagacatgc ccttccccgg cgtcgtggct taacagggct gccgcgaggg
     5761 tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta
     5821 actgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag
     5881 ggtgataccg gcaaacatct catccatcag gtcaatcaac ccggcgttgg gtcgggtcgc
     5941 gtagatccct tgttgcggat gttctttatc aacaccacgc aggaacagat aaataacgcc
     6001 gccaaagtgg cgctcatagt cgtaatcagc aatgcgatgg cgcagataac ggtgcagcgc
     6061 cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcattgccg ctgccatagc
     6121 ctgttgggtg taagcagaac tgtcttcacc caaccagttg gatttatagt cgagcaggta
     6181 ataacgccct tcgtggcgga acaccaggtc aataaagcct tttaacatgc cgcgtacctg
     6241 catgaactcc agcggcgggc agccagcgga tagcgagtca aactggcgaa ttagtgcatc
     6301 aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt
     6361 atcgcggtcg gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac
     6421 agccgtgatc cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag
     6481 tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact
     6541 gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc
     6601 gtcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc
     6661 gataccgtga ccacgctgct gcaaaccgga gtagctggtg acacgccagt tatcgccggg
     6721 caatcgttgt agcgtcctcg cattcagttc tgcagttata gcatcattaa cctgccaggg
     6781 ctggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat
     6841 acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc
     6901 actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac
     6961 gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc
     7021 cgccagacgt tcggcctccg cgagatcgac gctttctggc gcagcattaa gatccagaac
     7081 agcctcaaac gagtggcgat cgtgatagaa cgcctgatcc tggacgcgga aattggtaat
     7141 aaatggcaac cagaccagcg gatattccag cccttttgat ttatggatcg tgacaatctg
     7201 caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc
     7261 gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc
     7321 ttcttgtagc aattcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgctgt
     7381 tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac
     7441 gccacgtttg cgccagattt gccgataacc atcgaactct tcgacgaccg catcccacgc
     7501 atgttcgtca ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc
     7561 cagcgcactg cgcagggtgt tctcacgttc gggagtcatc accgcctgca acagccaaag
     7621 catctcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg
     7681 gatttccagc agcattaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag
     7741 cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc
     7801 gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt
     7861 actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg
     7921 ctgcgtttca cctttaaaca caaaacgtaa cgcctgattt ttgccggcag atttcacagg
     7981 aataaacggt atttcacgaa acatgaacgc gtcatcagtc tggctgaaaa gtttgttaac
     8041 gctgttcacc attcccggtg cggaacgcca gttggtatct aaggtgtagt gagcatgaac
     8101 ttcgctacgc gccttcatat aagtgaagat atccgcaccc cggaatgcat aaatagcctg
     8161 tttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa
     8221 aattcgatac tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg
     8281 cgtacggatc gccgctgcca atacctcgcc gctttcacta cgcagcgcgg aatcgagccg
     8341 acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc
     8401 gcggatctca gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag
     8461 cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg
     8521 atcttctaag aaacgctgag agaatttttc cagcgactcc ggcaactgat agctgttggt
     8581 ctcttcttct gcccaggcgc tgatcttgtc gatccattta gcctgattgc tacggttaaa
     8641 cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg
     8701 ccactgctgt tttacggcat caatacgcgc cacaatttgc gcgtggcggg aagcaagcgt
     8761 ttcatcatcg ggcggcggtg ctttgataac cggtgcttcg ccttgcagat aacgattaat
     8821 atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag accacctggg caatttcacg
     8881 cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga
     8941 ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag
     9001 catgcgctgg caaaaaccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc
     9061 taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc tcctccagca ggagtttgta
     9121 cagtggattg tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg
     9181 gatacgaccg cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc
     9241 aacggtcagc gggcggggaa aggcggcaga accgcctagt ccaagtaaca ggcgcaaata
     9301 gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc
     9361 ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca
     9421 tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat
     9481 gcacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt
     9541 gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta
     9601 tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac
     9661 ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gttattaccg
     9721 cctgctggat ttgcgcaaac tcctctggct tcatcgcccg caattttgcc tctgcggttg
     9781 ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa
     9841 ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca
     9901 actgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag
     9961 agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg
    10021 cggagtcggt gctgttaccg gctttttcaa agatgacaga ttgtttttta tcgaccacga
    10081 catctttgtt acggcaccac tccgaaccat cagcgcccaa ctgtttttgc acatcgcgtg
    10141 ccagcgttgt tgcctgggct tcggtcatgt tgccaataac cataaactct ggtcgagccc
    10201 ctgattttaa ggcgtcgcga taggccagca cctctttcaa agtgattgaa ggcaaaattt
    10261 tgcgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg
    10321 cctgctcgaa cgctttgccc ttttctgcgg aatccatcat ctggttatac caggacttcg
    10381 cctgctcaag ctgatcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga
    10441 acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt
    10501 tggtggaaaa actaatgcca ccaaccgacg cctggttgct taactgatca agcgctagcc
    10561 cggcaagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg
    10621 gattacgcaa aatcaggctg acatcagctt tgggttcgct ggaaaaataa cggcttggcg
    10681 catagaccac acgcagattc gactcatcaa caataagctc tggatggtca tatttcttct
    10741 ctgacttaat cagcgagaaa tcgtcaggaa tataggggtt aagctctggc aaagagagcg
    10801 caatgtcggc ggcttttttc tgccagtcag cgaaagtttg tgcgctaatt ttatcgacct
    10861 gatacggcgc atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc
    10921 agatacgcgc attctgcggc gtcatcatcg ccaggcgttc ttttactgct ttagcgtcgt
    10981 accgatcggc aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat
    11041 ctgccagcca ttcgacgtaa tccatatcac gggtgattga cggataacga aagtcgatat
    11101 ccagcacatt cgccagttca tcgaagtatt gtttatcaat ccctttttcg cgtaacagat
    11161 tgaggtagct aaaaatggcc gccacaacct gatcgcgatt cgccagacct ttatcggtta
    11221 aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg gaattggcgc
    11281 taatgccctc aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat
    11341 taccgatcag ataggtaatc aattcatccg ttttactacg gaacttcgct gaattgttat
    11401 cgatgcgaaa ctcaacgcgc agaactttac gcggcagcgc cgggacgtaa tgaatgataa
    11461 tacctttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt
    11521 tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattgc
    11581 tgtaaataac cgccttcatc agattggcag agtaatattt ctcgtgaaaa tctttcagcg
    11641 cctgctgcac cggatttccg ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact
    11701 ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt
    11761 cacgcgtacg cgccatggtt aattcggcat tcactgcatt acgttcacgt tcggcgtatt
    11821 tcttgtcgag caagggttct gcaatagcgt ccgccaggcg gtctaccgca ccgggcaagg
    11881 cgtcgttctc aacttccaga tagaaagccg tgcgatacgg tgccgtgctg gcattgtgac
    11941 tgccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc
    12001 ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca
    12061 gcgacccaac gggcactacc agcgccgaga gcgatttaac cgcctgcgga tcagaaacca
    12121 gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac
    12181 ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt aagggtgccc
    12241 aaagggcaac aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc
    12301 ataatcaact ttattttctg gccagattca acaggttaac gcaaatcatc tgccagaatt
    12361 taatcttgtg ctgaacgagt cagcctatgt ctatatatcc atcagtccgt gactggtgcg
    12421 catcataaag caagcagata tattgcgcaa tttttataca gcactcatga ctgattaaag
    12481 cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga tagcctccat tgtctctggt
    12541 gttaattgcc gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg
    12601 ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gtaacgtgga atcgtcatcc
    12661 agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt
    12721 aacaccagca atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa
    12781 tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa
    12841 aggcgacttt caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc
    12901 attccctgcg ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc
    12961 tgcggcaacc agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc
    13021 cccggctggc gacgtgcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt
    13081 tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg
    13141 aataagcgat cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga
    13201 taacggctaa gcccttccag aataaatggc tcggtgtcgg ggatttcgct gtcttcagta
    13261 cggaagttca cctgcaaacg catctggaag aacgcccgta ccggatgtgc ccagaatcgt
    13321 tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca
    13381 gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt
    13441 tcgcctggct ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc
    13501 gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatgg
    13561 ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta
    13621 tcctgaatag aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct
    13681 tccaggaaca gatagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg
    13741 ctcatcaggt caaagcccaa tggcgcaagc tggcgtggat aaacgccgtc gttcattccc
    13801 agcaggcaaa ccactttgaa tgggatggaa cgcattggca tcagagtaca aatgttaacc
    13861 ggtccggcga gaaaacgctg gctgatacgt tcctgatcca ggcgctgtgc cagttcatca
    13921 cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc
    13981 gcctgccatt gttgttcgat cagcgtcata gccgcttcgg tttccgcatc cggcaggaag
    14041 aaggcgttga gcatatcgcg acaaaccggc aaccactctt ccagcggacg ctcctgcgcc
    14101 agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct
    14161 gcaattaagc cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc
    14221 tccatcgcgt agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg
    14281 gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg
    14341 ttgacccact ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc
    14401 accggcacat ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat
    14461 aacaggctga taaacgcttc caataccgga tgtgactgcc gcgcccgacg gtcggaaatg
    14521 gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg
    14581 tagctgtcga tgtcggccac catcacgatg atgtcgcgcg gagtcagtgt cgggtcttcc
    14641 tccagcatcg ccagcaggcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa
    14701 acgtgaaagg tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa
    14761 aactcttcga tgtttacacc agcaacggcg cggttttcca gttccagaat gtcagactga
    14821 atgttatgca gcaggttatc tggcgtaaca tcgacaaaag cgtccagctc ctggctgctc
    14881 tccagatcag aaaggagata aatgtagtcg cgcccaagct tgccccatga ggccagcagc
    14941 gggttgccga catcctgttc accatcgctg ttaaagagct gcccggcatt ttcgctgtcg
    15001 cgaaatagcg gtaattctcg atcttcaaaa ctgtgtcggc gctggcgagt cagtagtttc
    15061 gccagataag caggatcttt aatgtcgccc cagtaataac ggcaggggtt ggtaaacagg
    15121 agatggattt caatatgttt acccagcgcc tggagtgcct ggagataaac aggcggtaac
    15181 gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac
    15241 tccagcgttt cgataaagcg ctgatagaga ttggcgcggt gccagcgcgg ttgcccgagt
    15301 tcatgggtat attccaccaa cgccttccac aacggcgctt gccaggcctg tgcttctccc
    15361 agcccttcaa ccaaatgtcc tatttcccac tgtgccagcc agtccggacg atagaccaga
    15421 tactggtcaa acaggtccgc cgcttttgaa gaaagctgga acagttttcg cttgtcgcta
    15481 tcgtcagtca gataatgccg caacagggta aagtcttcgc gctccaacag ttgcggcagc
    15541 agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc
    15601 ggtaacaccc ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt
    15661 gccgcaatac caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt
    15721 tgcaccagaa tcatttctgg ttcgaaagga tcgtccagcc gttcgcgttc gacgataaac
    15781 tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct
    15841 cctgactact gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt
    15901 gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc
    15961 tgccagttag caggtggcga aatcgcgcgc agttgcgttt gctgccagcc atgccgccag
    16021 agctgttggt actggtttct gctggcaaga ctgttcatta atgttcgctg gaaacccgat
    16081 aacgcagtga caatcatcac catcaacacc atcgccaaca ttacctccgg cagactaaag
    16141 ccttgttgat tcctcaggga agctgacata acgccctctc tttcagcgga caaaaatcgc
    16201 tccagccgcg tggcgaaaaa acaatgtttc catcgatgac ttcgcctgtt cgccacaacg
    16261 aaacgccttc ataaccagca atcaataagg cttcattatc tgccagtaaa cgcagacaaa
    16321 cccgggcacc ggtggcagca tacagtaagc actgaactgc gggcagcgtc tgccaggagt
    16381 gcatttttcc ccactccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt
    16441 ccatgctcac gcgagaagca aaactgcgat cctgctgact cattccttgc aatagcaagc
    16501 tacccaaaat cagcagcatc aggaccagag ccagtgacga aacacccttt tcgcggttca
    16561 caggttggat cctgtcacgc tatagctggc atccaccacg gtttgcggat cagccttgct
    16621 ggcagcgtgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac
    16681 ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc
    16741 acaggatgtc gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg
    16801 gtcggactct tttaccggtt cgcgatccca gataccatta ctgttcgcat cccactgcac
    16861 aatgacacag tcaccctgtc cgacaatttc cagcccttcg cccgtacagc tgccatggca
    16921 ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc
    16981 cagcgccagc ttacgggtgt tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc
    17041 agcccccagc aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa
    17101 accttgctct tttacaggca tccttctgtt tctccttgct gacaaagccg gagtcttccc
    17161 cacggcgaaa ccactagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc
    17221 catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact
    17281 tcgggccagc gcggcacaaa gaccaatggt gaactgccat gacaggtatt cgccccagca
    17341 acggaactca caaggcacca taacgtcccc tccctgataa gactgatact gtggtcgcgg
    17401 ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt
    17461 tgccaaagcc gttgcgactg ctgccagtat tgccagccat aaagcccact tgcgcttagc
    17521 atgaccagaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc
    17581 atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggca
    17641 catcttccgg actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggtgtctcga
    17701 agaatttaac ggagggcaaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt
    17761 tcgtaattag atagccaccg gcgctttaat gcccggatgt ggatcgtatc cttcaatctc
    17821 aaagtcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg
    17881 cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatggt tgctgtagag
    17941 atgcgtgtcg ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat
    18001 catatgcacc agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca
    18061 ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc
    18121 atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat
    18181 gcggcgggaa tccgggtcgt ttttcagctg gttcagtaca gtagtgatct ggtcaatatg
    18241 acgaccatcc ggcgttggcc aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc
    18301 gttttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt
    18361 agtgtcgccc tgcaggaacc acagcagttc gtggatgatg gaacgcaggt ggcaacgttt
    18421 agttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga
    18481 aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat
    18541 cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata
    18601 tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat
    18661 gctgatgtac tgcacccagg caccagtaaa ctgcgcgtcg ggctggcgga aaaactcaac
    18721 aatgatgcga aacgcgccgt aaccaatcag gaacaagcct gagacagctc ccattgggcg
    18781 cggtttacga atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc
    18841 gtaaagctgt gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg
    18901 cggattggtt tgcagcagca aaatatcttc tgtacgggag ccagggaaca gcatggcaaa
    18961 cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccaggcgccc
    19021 ggcaccaaga ccaaacggaa tgagtggggc aataaaatca gagacctgga agaaggaacg
    19081 tttagtacgg cgggcgaaga taatcatcac cacgataacg ccaatcaggc ctccgtggaa
    19141 ggacatgccg ccgtcccata cacggaaaag atacagcgga tcggccataa actgcgggaa
    19201 attgtagaac agaacataac caatacgtcc accgaggaag acgccgagga agcccgcata
    19261 gagtaagttt tcaacttcat ttttggtcca gccactgccc ggacgattcg cccgtcgtgt
    19321 tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc
    19381 cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt
    19441 catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcacgc
    19501 cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc
    19561 ggataccgtt aaaaaatatg tatatgatcc gcgctataac cccccgcgaa tcaacccgcc
    19621 catgccgcga cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc
    19681 ttccagacta cgctgcgcaa gattttctgc ttcagcaaaa tcaatgcgcc gcagcaggta
    19741 ttttacgcgc gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag
    19801 gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg
    19861 tatttccgct tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc
    19921 ataaatgttc gccacccggg tattgttgcg atcaacggcc aggatgtatt gagtcagatc
    19981 gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat
    20041 tgacggcact tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc
    20101 ctcgacttca cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt catcgaggct
    20161 tgtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc
    20221 ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc
    20281 caggcatgga ttctcttcgc taatcggcat gtaaggcagc tgcttatctg ctccgacatc
    20341 cagcgtacgc aaggtgacgg gtttatcatt aaacatttgc agcatcccct gatactgcgc
    20401 cacctgttct tcttccgacg gaaaaccact ttgcagcatg aatgggattt cagtgcgata
    20461 aagtcctatg ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccggc
    20521 attgagcatg actttaatgc gctcgccgct ttttaactgg gcgggtaaat tgacgtcatc
    20581 ttccgccaga cggctaagct cgatctcttc actaattagc cgctgatatt cttgcagcag
    20641 taccggttcc ggatcgacca gcaattcacc gcgataacca tcgacgatca gcgtccgacg
    20701 atgcagtacc gaaggctgaa tatccgcgcc catcacggta ggtatcccca gcgcacgtac
    20761 catgatcgca gcatgggagt tggcagcgcc atcgcgcacg acaacaccga ctaagcgatc
    20821 ctggggcagc tcagcaagcg ttgtcgctga cagttcatct gccaccagaa tgaaacgttc
    20881 cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag
    20941 cgcacgtaaa tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc
    21001 ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc
    21061 ggcaaacaat tcgcgacgca gccgggtatc cgaaagcagg tgtgagtaaa gatcgaaaat
    21121 agccgccgtt tctttttgtg caccggcagc aaagcgtttg ctatagcggc gaaactcgtt
    21181 agccgcctct tccaacgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga
    21241 tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc
    21301 cacaccaggt gctgccggta atgcacggat tcgcgtctgg cgatattgcc caaacaaggc
    21361 agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga
    21421 ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa
    21481 ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga
    21541 ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag
    21601 cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg
    21661 ttttttcagt ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta
    21721 gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc
    21781 ctcattcagg cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat
    21841 aattggcgtg acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt
    21901 attttcctgc agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc
    21961 acgtttaaat gacaccacct gtctgactgg ataccagtaa cttacccatc gccagccgtc
    22021 aaactctggt gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa
    22081 gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg
    22141 tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg
    22201 gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg
    22261 gttgattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac
    22321 ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat
    22381 caccggacta cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga
    22441 cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac tcacagttat
    22501 gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga
    22561 tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct gaataagaca
    22621 gatacagaga ttttaaaata ttcattatca gtcaatgcat tacatgtgat ttgagtaata
    22681 aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac
    22741 caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg
    22801 gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tgaaggtttt tcacagttat
    22861 cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg
    22921 aaaacccgtt gcagtgttgc gcaactcgat taccggcaac ctaaaaagca atataaatca
    22981 gagaattgaa caacgcatgt ggaaaaagtt acactgcgaa tattcggcac ttaattgctg
    23041 tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc
    23101 gaaactgaag aacaattgtt agcgcaagca caacaacttt ctggttatac attgggagaa
    23161 ctggcggcac ttgccgggct ggttacgccg gagaatttaa aacgcgataa gggctggatt
    23221 ggcgtgttac tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt
    23281 gctgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa
    23341 acaacattcg tttgtgttgc cccgttaacg ggcaatagcg gggtgacctg ggaaaccagc
    23401 cacgtgcgcc acaagctcaa acgcgtgctg tggataccgg ttgaaggcga gcgcagcatc
    23461 ccgctggcaa agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac
    23521 cgacagttac gcgaagactg ggaagaatta atggatatga ttgttctcgg tcagattgag
    23581 cggatcaccg ctcgtcacgg ggaatattta cagatacgac cgaaagcagc gaatgcgaaa
    23641 gcgctgaccg aagctattgg tgcccggggc gaacggattc tgacgctgcc gcgcggattt
    23701 tatttgaaga agaatttcac cagtgcgcta ctggcccgtc attttctgat ccagtagcca
    23761 tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt
    23821 tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacactga
    23881 cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa
    23941 agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtca
    24001 tgcgtctggc gctgctggca tccatcgcct gggttacgcg cctgacgaat ccgcttttta
    24061 caatattcag tcaggaaatt tccgcccgtg atttgatcct gcttctgggt ggcttgttcc
    24121 ttatctggaa agccagcaag gaaatccacg aatccatcga aggtgaagaa gaagggctga
    24181 aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct
    24241 tcagcctcga ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg
    24301 ctgccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg
    24361 tcgagcgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta
    24421 ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt
    24481 tcttctctat tgcggttgaa agcctcaacc tgattcgcaa caaaaagaat ccgctctgat
    24541 actccgtacg ctctcctgcg ggagggcgta ctcactcctg ttaccctcac agtttaagat
    24601 tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca taggtgggcc
    24661 aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt
    24721 tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt
    24781 gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca
    24841 aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat
    24901 aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca
    24961 tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata
    25021 ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag
    25081 aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt
    25141 atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaactcaagg gctaaccgaa
    25201 acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc
    25261 aaagtgagcg gaccgtcgcg caataatgac aaaggcatcc gcccggatca ggcgctggat
    25321 cggaagaata tccgcgaagc gctgcatgac agcctcaagc gtctgcagac tgattacctc
    25381 gatctttatc aggtgcactg gccgcagcgc ccaaccaact gcttcggcaa actcggttat
    25441 agctggacag attctgcgcc tgcggtttcg ctgctggata cactggacgc actggcagag
    25501 taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta
    25561 atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac
    25621 ccctacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa
    25681 ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc
    25741 aacggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc
    25801 ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tagccagacg tcatggcctg
    25861 gatcctgcac agatggcgct cgcttttgta cgccgtcaac cgtttgttgc cagcactctg
    25921 ctgggcgcaa ccacgatgga gcagctgaaa actaacgtcg aaagtttgca tctggagtta
    25981 agcgaagacg tattagcaga aattgaagcg gtgcatcagg tttacactta tccggcacca
    26041 taagattaac gccggttaaa accggcgtta aatattaata acggcgctgc cagatccaca
    26101 gcgccgttat tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga
    26161 tgcctaccat taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc
    26221 caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc cgttcctgta
    26281 gcaacgcatt gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg
    26341 cataggctga cagcagctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa
    26401 tcccggctgg catacagcgt gacacggttt ccagcgtaac taacttcgct gccgcaccag
    26461 cgccaaccac gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg
    26521 taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc
    26581 cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg
    26641 tgcaggcatt caggaaactg cgggtcatgc tgatgagatt ccaggactgc cccggacgcg
    26701 ccgccgccag tttaggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc
    26761 atgcggccag ggcgaccagg acatgccagt cagccagtac accaccggct acggaaccga
    26821 gcaaaatcgc cgctatggta gaagcttcca ttaaaccatt agctttcact aacttactac
    26881 ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac
    26941 ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct gcgcccagca
    27001 gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgtc
    27061 cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc
    27121 tccactccgg ataaaactgc gctttcagta acgccagagt ggcaaacagc agcgcattat
    27181 cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg
    27241 aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc
    27301 aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata
    27361 tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg
    27421 cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc
    27481 ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt
    27541 tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc
    27601 acaaagccct gctcgtcgaa acgcacaata tcgccagtgt cataccagcc gcgctccatt
    27661 tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga
    27721 tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccagga
    27781 accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc
    27841 gccatcggta cgttgataga aacgacaggc gcgcactcgg tcacaccgta gccttcaagg
    27901 atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg
    27961 cctgccacca catagcgtag acggtagaag tcatacgggt tggcgaaacg cgcgtagtga
    28021 ccgagaaaag tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caactccggc
    28081 acaatgcggt aatgcagcgg gcttggataa aggaacacct ctgcacctgt gagcagtggc
    28141 gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga
    28201 tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta
    28261 tggctatgga cgacgccttt cggatggcct tcagaaccgg aggtaaaaag gatcagcgcc
    28321 tcttcttccg gctgctgttt tacctgtgcc agacgcggca tcagcaaatg agcgaagatc
    28381 catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc
    28441 tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg acgggaagtg
    28501 aaaatggttt tgatttcagc cgccgtaata gcactggtca acccttttac cccggcggtg
    28561 tagttcatca ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg
    28621 atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg
    28681 atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat aggagtccgg ggtaaagtta
    28741 acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcgctcag taaagattcg
    28801 tacagcgttt cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt
    28861 tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt
    28921 ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg
    28981 cggctgaagt gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac
    29041 ttcgccgcga caaatcccgc gccatcgtag attttcatca gcgagccagt ggtggtgatg
    29101 cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta
    29161 atcgccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc
    29221 atataccact gttggcttat tgaggtgtaa acggcaaata ccggacgtac gggtaaaaac
    29281 aatgccagca gtatgccatc aataaaagag acgtgattag gcgtgattag aacgcgctcg
    29341 cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt
    29401 cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag
    29461 ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc
    29521 gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca
    29581 atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gtgagaaaac
    29641 gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg
    29701 atgaccg
//
LOCUS       DAZGTJ010000064        29602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000064 DAZGTJ010000000
VERSION     DAZGTJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.064"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(145..9909)
                     /gene="cdiA"
                     /locus_tag="ACHK8E_003344"
     CDS             complement(145..9909)
                     /gene="cdiA"
                     /locus_tag="ACHK8E_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFP0345568.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAKPFEKTIYDPKIFTDEKMLQLGQEAAAIGYSNAIKNGLQAYDAKAGG
                     VTFRVYIDQKTGIVSNFHPK"
     gene            complement(9922..11700)
                     /locus_tag="ACHK8E_003345"
                     /pseudo
     CDS             complement(9922..11700)
                     /locus_tag="ACHK8E_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            12200..12589
                     /locus_tag="ACHK8E_003346"
     CDS             12200..12589
                     /locus_tag="ACHK8E_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345569.1"
                     /translation="MKRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            12653..13711
                     /locus_tag="ACHK8E_003347"
     CDS             12653..13711
                     /locus_tag="ACHK8E_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP0345570.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            13752..14474
                     /locus_tag="ACHK8E_003348"
     CDS             13752..14474
                     /locus_tag="ACHK8E_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFP0345571.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            14471..15292
                     /locus_tag="ACHK8E_003349"
     CDS             14471..15292
                     /locus_tag="ACHK8E_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP0345572.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            15267..15524
                     /locus_tag="ACHK8E_003350"
     CDS             15267..15524
                     /locus_tag="ACHK8E_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFP0345573.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            15536..15787
                     /locus_tag="ACHK8E_003351"
     CDS             15536..15787
                     /locus_tag="ACHK8E_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0345574.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            15792..16373
                     /locus_tag="ACHK8E_003352"
     CDS             15792..16373
                     /locus_tag="ACHK8E_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345575.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            16370..17728
                     /locus_tag="ACHK8E_003353"
     CDS             16370..17728
                     /locus_tag="ACHK8E_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFP0345576.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            17715..18068
                     /locus_tag="ACHK8E_003354"
     CDS             17715..18068
                     /locus_tag="ACHK8E_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFP0345577.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            18059..19735
                     /locus_tag="ACHK8E_003355"
     CDS             18059..19735
                     /locus_tag="ACHK8E_003355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0345578.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            19739..20161
                     /locus_tag="ACHK8E_003356"
     CDS             19739..20161
                     /locus_tag="ACHK8E_003356"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP0345579.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            20158..20763
                     /locus_tag="ACHK8E_003357"
     CDS             20158..20763
                     /locus_tag="ACHK8E_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFP0345580.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            20732..23050
                     /locus_tag="ACHK8E_003358"
     CDS             20732..23050
                     /locus_tag="ACHK8E_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFP0345581.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            23047..23631
                     /locus_tag="ACHK8E_003359"
     CDS             23047..23631
                     /locus_tag="ACHK8E_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFP0345582.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            23633..24802
                     /locus_tag="ACHK8E_003360"
     CDS             23633..24802
                     /locus_tag="ACHK8E_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFP0345583.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            24799..25263
                     /locus_tag="ACHK8E_003361"
     CDS             24799..25263
                     /locus_tag="ACHK8E_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFP0345584.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            25263..25994
                     /locus_tag="ACHK8E_003362"
     CDS             25263..25994
                     /locus_tag="ACHK8E_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFP0345585.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            25991..27220
                     /locus_tag="ACHK8E_003363"
     CDS             25991..27220
                     /locus_tag="ACHK8E_003363"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFP0345586.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(27394..27663)
                     /locus_tag="ACHK8E_003364"
                     /pseudo
     CDS             complement(27394..27663)
                     /locus_tag="ACHK8E_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28060..29091)
                     /locus_tag="ACHK8E_003365"
     CDS             complement(28060..29091)
                     /locus_tag="ACHK8E_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0345587.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            29362..>29602
                     /locus_tag="ACHK8E_003366"
     CDS             29362..>29602
                     /locus_tag="ACHK8E_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0345588.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLAL"
BASE COUNT         6517 a         8147 c         7682 g         7256 t
ORIGIN      
        1 tataagattc tttgttaact atattttcta cagcgtccaa gaacctattt tcattataca
       61 taaggtcaaa gtatgacctt atggtccacc caggttcaga acgttcatat ggcaattcaa
      121 ataaatattt attcatagtt actcctattt cgggtggaag ttacttacaa ttcctgtttt
      181 ttgatcaata tatactctga acgtaacacc accggctttt gcatcatatg cctgtaatcc
      241 attttttatt gcattggaat aaccaattgc cgctgcctct tgaccaagtt gcaacatttt
      301 ctcatctgta aatattttag gatcataaat agttttctca aaaggcttgg cgccatttcc
      361 tttataatta ccagtcgtat tcccagcagc atcttttgtg gggatttcat actgaacctg
      421 agtaatacct ctgactcctg ttggtgtttc agaaataatt ttcacaccat tatcaaccac
      481 agctttattg aatacatcag cattatgtgc accgctaatt cctttcttct gcgaatatcc
      541 gtcaagttga gctaagtgtt ccggctgatt aattttaaaa ccaatccctg aaggatattt
      601 attcgcttta tcaaccaatg cagtaatttt attagccaca gcctgaccaa ataatggtgt
      661 cattgaagcc gctgcaattt ctaatccctg cgtcgtctcg acataacctt gtgctgtttt
      721 ctggtcaacc cccagcgttg ccatgaagta actcaccatc gcatctttca cctgctgttg
      781 attttgtaca tcaacactgg tgatatcgag gagattgact atctgaccat acgcatcagc
      841 gtattgctgg ctgtatttcg agttataagg gccatcttcg tacccttctt tcgacgcaat
      901 aactttcagt cttgcgctgg cacacgctga actacccgca ttaccattac cgcaggcagc
      961 aataacctcc ttatcactgg caatatcttt ttcaagcaat gcatcatatt tctgctgtgc
     1021 cttttcacgc tctgccggat ttttactgtt tttaagcgtc tgttttgcta tctcaagctc
     1081 tgtcttttca gacacgctca ggtagttatt atcaacagca ttcttcccgc tctgtgcgcc
     1141 cactgctgct gatgcgctgc tgtttccggt aagaccgcca gccagccctg ctgacacggt
     1201 tgccagcgta cttatcgtct gcttctgctc ttcactgagg tctgactgtt tcacgcccgg
     1261 atacagcatg ccggcaatcg ctctggctgc catctctccc gttgctgcgc ctgctgcacc
     1321 ggcagcaaca ttattaccct gcattgccgc tactgcgcca cccagaatgg cgtgagcaat
     1381 ggcatttact accggatctt tttctgttga tttcagcagg tgcgccagtt ccggggccga
     1441 agctccggcc agagcacccg ctaaattccc cccagccagt ccctgaagcg cggctgtcgc
     1501 ggcctgtata ccccgctgaa tctcactgcc agtgccatac ttttcctgtt cctttttgta
     1561 ttccggcgta tcacgcagtt tcgcaagata ttcctgacgt tgtttctctg ttgcattagc
     1621 aggaagtgtt tcgccggttg cttccttcgc ggccttcaac gcattcagtt ccccctgcgt
     1681 ccgcgcgata tccgccacct gactgcctat gtcactgata agccccactg tctgcagacg
     1741 cctctgctcc ttctccttgt caaatatcgg gctgatactg tcattagcgt gcgcaggatc
     1801 acggctgagg tctgccggat tctgcttctg attgtccctg tcccggatgg tgatagtgcc
     1861 ttctgccact gcggcctgag tcgttccttc cgcatgtccg ctgtgacctc cggcggatat
     1921 catgccaccc ggcatgttac cctgaaattt atccccgaag ctgccaccac cgctcagact
     1981 gatcccactg tgactgactt tataatccgc ttcgttgtga aggtcactga accccagcgt
     2041 tccggtatcc aggtggtttt tatccggtgt ggcagtggag gcaatcaccg caccatccag
     2101 ttgggtatgt ctgcccactg tgatgtcgaa gccaccatca ccggcaaaca ttccggtttg
     2161 ttcagcaacg gagtcaaagc ggctcttcat cttatcccgg gaggcagcga tgtaacctga
     2221 gccggtcatg gagccaaagg taaaactgcc gccggcagcc acgctggtct gtttactgtc
     2281 gtacttactg gtgtcctgct ggctgctaat cagcaggtcg tggcccacat cggcgataat
     2341 cctgttgccg ttgacctgag caccgttcag taccgtatcc cgaccactgt tgatggtgac
     2401 ggttttaccg ctgtctgttg tggtttcagt ccactcagta ccgttacctt tctcgctgcc
     2461 ttttgccgca ttaacgctgg caaagacgct gataccggca ccgttaccac cggcaccgat
     2521 actgacaccc acgccaccgc cactgctgct gttcctgccc gttgtttttt gtgtgtttgc
     2581 agcgccactc aacagaatat cattcgcggc atccaggctt gtgttaccac cggccttaag
     2641 ctggcttcct gcaatcacaa tatctccgcg gttatcgccc ctgtttttgc cggttgcgac
     2701 aacagacaga ttattcccgg cattcagcgt actgccggat actgtgtcac tttcagaatg
     2761 ttgttgtgat ttcgatttct gagtggtgag cgacaggctg actcccgtcg cattcgggtc
     2821 accggttgcg gtggccattg ccgcagcctg tccggcctgc acaccagaca gcgctgtctt
     2881 tgtggcctgc agggctttca gacgactgtc actgctctcc ttcgtttcct gtgcactggt
     2941 gaccgcatta ttgatggcac tgcccactgt gccggaaagg gcaaccgtca gcccgctttt
     3001 cttctgctca aatttttcgt ccacagtacg acggtcatgc cccgggtcaa ccaccacact
     3061 gtcaccggta atgctgatat cccggttcgc aatcacatcc gaaccgctga tatgagcctg
     3121 tttgcccgcg gtaatactga cattaccggc agtggagccg atggtactgg cactctgact
     3181 ctgcgttgtc ccggcctcgc ggcggtcgtg cgttgtctta ctgctgccga tggtgaagcc
     3241 gataccgcca gtacccatca gacccgattt cttcgtttcc ttaaagcgcc aggacgtatc
     3301 tgtactggtg acagcaagaa catcaacatg gttacccgcc gccagtgaca catcccggtc
     3361 agccaccaca tccgaaccct ctaccgtcag gttatcaccg gcgttaacgg tcacgcggtt
     3421 ccccgacagc agggagcctg cttcacgggt ggcactgtcc tcactgatgg tgtgggtggt
     3481 tttcttactg agaaaacctc cgcttttttt cttcgtttcc agatagtgat agtcactttc
     3541 tgtcgccgtg gtcagggcaa catcacgacc ggcattcacg ctgatattgc cggttgcggt
     3601 aacggatgac gcaacagcgg tgatatcccg tcctgcagta atggtggtgt caccaccgct
     3661 ggcgatttcc gttccctgct ggcgaacggt ttcatcaatg accgttttct ttttcgagta
     3721 tgaactgctg ccggtcacgg actcttccgc cagcagatta acgtcgcgcc cggcccggac
     3781 agtgatgttg tcttccgctg cgatacctgc accctgactg ctcacatccc ggcctgcact
     3841 cagcatcaca ctgtcgccgg cagtaatggt cgtaagcccg gcgctgcgct ggtgagactc
     3901 actgtttgca tgacgtctgc tgcttaccct gtcaatggca ttcagactga gatcattgcc
     3961 ggcaataagt gctgcgcggt ctccggcact gatttctgcc cctgtgaccg aaatgttttt
     4021 atctgcccgg atgtccagac tatccatggc actgacagag gcaatgcttc ccgtataagt
     4081 gtgtttcagg gcgaggctgt cttccggtgc atcaagctgc cagtgtgagt accgggtgag
     4141 attattgaca ttgccggcga gactctccag ccggacagtc ttaccggtaa tcgtggcact
     4201 gatattgttg atatcgccca tcgctttcag tccgagagcg ccaccggcat ttataagccc
     4261 ggactcaagg ttacccagac tgtcagcgct gtcgatggtg agattattct gcgccatcag
     4321 ggtactgcca ctgttagtga cattccctcc tgccaggtgc acattctgac cgctgataat
     4381 actgccgttc tgcagcgtca catctttcgg tgacagatac acctccggca ccatcaccgt
     4441 ctgacccttt atcgtgacgg ctttatacca cagaatactt tggtcgagag ccgccacctg
     4501 gtctgctgtc agtgccacac caaacttcag accgagcgcc ttctgtgcac gggcagcgtt
     4561 atccatcagg tagcgcatct ggtcggtgtc tgaaccaacc ccgttgatat agcgcccccc
     4621 gatctggttc agcatatagt tcgatacata gcgggtatca aaagcggcat cccccaggaa
     4681 gcgataatct ttttccggtt tcagcccgag ccggtcaaga aaatacgatg agcccgggaa
     4741 ttgtttttca tcagtatacg ccgggtctgt ttcacgtggt gcctgcccgg ggtgcatcct
     4801 gagcaggtca tacagtccgg taaacagact gctgtccacc tttccgagac catccagttt
     4861 cggattcacc gtaatcagat atggactgtc agggtccgtg gacgggacaa aataaccatt
     4921 gttaccggaa ggcaggggat agttcccctc cacgatgccg gaagccaggt ccgtgccact
     4981 gccgatcgtc agacgggtaa tggtgtcagt ccagtccggg gctgtgacgg tcagcgtctc
     5041 tgtgccatcc ggcgccagtt gagccattcc cgggctgata tttttctctg ccggcgtgtt
     5101 aatttcaggt gccgcaaggg tatttgtgat ccgtcctgca tttgaggtgg tggatgtatt
     5161 actgatatca ctggaaaaaa cggcagtgac atttttgcct gcctgaatca cggctctcag
     5221 agcctgaccg gtgtctctgg tgacatattc cggagctccg gagagagtaa aggtaacgcg
     5281 atcatcggca gaagcatctc catactgcag atgcccgtcg ttggggatca tggccagttt
     5341 gccgtaatag cgatacacat tgtattcatc ctgtgtatag ccaaagaagg actgattatt
     5401 cagttgtgaa cccgacaggc tgacaaaccc gctggcaaga agatgacttc ccgtattatc
     5461 cagtttgtcc gcgtcaatca gcagattatt cccggccgaa attctggcat ccccccctgt
     5521 tgcaccaaca tcgacgcgtg ttgtgccagt cagaaatttc cgggtggctc ccttttcagt
     5581 cggaacggcc catgcgtcaa aaccaccacc ggcagtaccg ctccaggact tcacataata
     5641 tccccatcca tcttcaccta aatcgcccac tctgactgaa acagaatttg caccatcagg
     5701 tactgcgaca ttctcaaccg gaatgtatga ttttgtctca tttatgccct cccgctgatt
     5761 cagtaaatgt ccggttctga tggtgatatc accgcgggtg gtttcgatat tcccggaggt
     5821 attaatcact tcagcattcg cggcaccact gacatctttc tgcatcacca ggctgtcacc
     5881 cgccagcata tcgccgtgat ggttccggat ggtgctggca aacagcgaga ggttattgcc
     5941 cgcatacaac agtgcggtgt taatgactgc cccggcggcc gttaacatca gactacccgt
     6001 cgtgccggta aaagcgtcga gagtgatatc tcccctgctg gtcaggctga cgtccccgcc
     6061 tgcctgcagg ctgccgctgg cattcatcgc aatcccgctg ccgctcagtg ccgtggtgcc
     6121 gttgccggcg gtcagctggc cattgttggt cagtctgcct gcagcattca gcgtgatacc
     6181 gttgccctgc aatgtggcgc cattggtaat atccccctgg ctgctgacgg agagttgttt
     6241 atttgcggcg ataacgccct gtgcggtcaa accacgggtg agcttcagcg tcaggttacc
     6301 gagtgcaacc agctggcccg cgccgctgag cgaaaccatt tcagcaagca tatcaccgcc
     6361 gctgaacacc ttcccgccgg cgttgttcag gttgttccct tttattgtca ggccctgatt
     6421 acccagcagc gtgccgctgt tggtgatggt ctgcgtgttg acgttcagtt gtgcggcctg
     6481 gaacagacca cggttggtca gactgttacc tgtgagcgtt gcctgattcg ccgccatcac
     6541 agtgccatta ccggcaagct tcgccacgtt tagcagcagg tcagtggcct gtagccagcc
     6601 actgccactg tagtccggcg tggtgaacgt cagctcaccg ccggaaagaa tcttgccact
     6661 gttgtgggcc tgcgttgccg cgctgagcgt ggttttaccg ttgccctgaa tggtgccgtc
     6721 attggtcaac gcgggtgcgg aaagcgctga tacgttctgg ctgagaagca ctccgctggt
     6781 ctggttggtc agggcattca gcaatgtaag agtcagcccg ctctcgccct gaacacgacc
     6841 tgagttggcc agcgaactgg cggtgattcg gttatccgcc ccctgaagct gcccactatt
     6901 gctgatgtct ctggcgttga cgtttaatgc accgcccgtc agcattttac cgtgctgttg
     6961 cagattacca ctgagtgtca cgttcaggct gttaacccca ctgatttcgc cactgttgct
     7021 gagcgaatcg ccattcactt ccagcgtctt cgcgctgatt aatccctggt tacccagcac
     7081 tttgccgctc agacgggcat cgcccagggc ggtaattttg ctgccggtgg cggcaataac
     7141 atcgccactc agggtcagag ccaaatccag cgcactctga acagcaccgc tgctggtcag
     7201 gctgctggca tccagagtga tgttctgccc ctgaatccgg ccgctgttgg tcacatctcc
     7261 tgcgctcagc accagcgagt tctggctcag caacgcgcca ctgttggtaa gcccgtccgc
     7321 gctgtgcagg gtcagaccgc tcagccccgt gaccgtaccg ctgttgtcag tgcttctgtg
     7381 gtgaagcgtg acatggttac cctggacagt gccgctgtta tccagtgagt ttccatcaag
     7441 ggagagcgtg ccggccgaaa gcagactgcc ccggttgtcc gtggtggctg cgttcagcgt
     7501 ggtgtcaccc tggctcatga tatcgccggt actggtcaac tgaccggttg ccgaagcagt
     7561 aaggttaccg gttgccagca cggaaccacg gttcgcccat ctgtccgcct gcacggtgag
     7621 attctgtccc tgcgtggtcc ctgcggtatt cagtgtttta ccccggaggg agaggtcgcc
     7681 cgccgtcagc cagcgtccgt gacgaccctg tgagagggtg tcgcctgcaa gcgccagtgc
     7741 agcggcgccc tgcaacaggc catcgccatc cagcgtggtc gccctgacgt tcagcgtgtc
     7801 agcggtgatt ttacccggat tgctgaggga ggcagtgttt aacgttaaac cattatcacc
     7861 ggtgataagc ccgctgttgc ggatgtccgg tatatccagc gtcaggtctg cagcactgta
     7921 cagcgtgccg ttctgctgat tatcaagcct ctgtgtgtta acggtgagtg aggcctcccc
     7981 ctgcaacaga ccgctgttgg tcagggtctg tgactgtgta ttcagggcgg aagcaacaag
     8041 tacgccgctg ctggtcagtt ccggcgcact gaggctgagc gacggggcgc tgcttttccc
     8101 gctgtgggtg agcttttcac tggcgttcag ggtgaggata tctctggcag cctgtgttcc
     8161 gtcaagctgt gcgttctgca catccacgct gacactgttg ccctgcgtct gcgcagtggt
     8221 cgtggtcttc agactgttgc cttttacaga caccgtgcca cccgcactgg tttcacccgc
     8281 cagtgtggaa gacgcgctgt tcagcatgag gttaccgtca ctgtttgtga ccgatttttc
     8341 tgtggtggtc agtgtgtctg cagaagccac aagttccttt gccccggaga gggtaccgct
     8401 gttgtttatc tgcgtggcac tgagcgtcag cacgtcatcg ctgatgagtc tgccgcggtt
     8461 ttcgagtgta ccgctgacgg taacgcgggc actgtctttt gcaccggcat caccggacag
     8521 tgtggcattg cgggcgctga tatcgagtgt gctgccactt ttaatactgc cggtgctggt
     8581 caggtcagtg gctgtgactt tcaggccgtt cccctggacg gcaccggtat tactgaatgt
     8641 cccggttttc accgtggcat cgcgcccggc aaccagtgtc ccgccgttgt tcaggctgcc
     8701 ggagctgacg gtgagtgttt tcccggcaac gctacggcct ttcagtgtgg tatcatcggt
     8761 ggtggtaagg gtgacatttt cagctgcatt cacggccccg gaagtctggt tcaggtgcag
     8821 tgcactgagc atggcgttct gtccggcagt cagttcacca ccgttctgta caatctgccc
     8881 ggatgaagac agcaccagat ttttatccgt caccagacgg gactgattca gtgtcagtcc
     8941 cgttgtcccg gtaacgctga gatttcctcc ggctttgtta tcccctgaaa gtgagacatc
     9001 cgttccggtt acggtggtac tgccgccggc aaggctgttc ttcaggacca gttttccgga
     9061 actgctcagt atgatatcgc cctcgcgggc ataaaggtta ccaagattaa ccccgacacc
     9121 actttcagtg gaggtcagat gaatacgcct ggcgtacatt ccaccgagag cgccggtatc
     9181 aacggcaact ttcggcacat taccttcgcc cttcagggca ctgacgcgac catctgcagt
     9241 tatccggtta gcgcctgcag tgacagttaa atccttcgca tgaagcgcgg cattcacttc
     9301 cgttgcacgg gcaataatgg atacggcatc gctccaggtg ccgtccaggc ccgcgccatt
     9361 gatggtgata ctgccttcag tcacctccag cgcctgcagg ctgccgtcgg cattcatcac
     9421 aggtttgcct gtggtgagcg tcgcgtgcgg cgtgttgata aaaccacagc cgtcacaggt
     9481 gataccatac gggttggcaa ccatcacatt cgccgctttg ccggccactt ccgtatagcc
     9541 ctgcagcaat gaacggttac cgccagtcac ttcgttgatg atacccttcg cttccccgcc
     9601 cgctttcagg ttcgggttat tctgtatcag tccaccaagc tgcgtcggat taagcttacc
     9661 ggtggcatta ttgagaatca gcccttcctt cccgacgttg taatccgtaa accggttatg
     9721 cgaaatcccg gccccgttcg gcgtggcaat gttcacgacc ggcacaccat ttgccgcttt
     9781 atccattccg gccccgtttt gtggggtgat gacggccccc acagccggta acaacggctg
     9841 cccggcgata atcgtactga taaggtaact cagcaggcgg taagtgaagc gaacgggagg
     9901 ctgatgcatg gtaataatcc cttaaaacgc gacggcaacg cgccagtaaa ccgtgagatg
     9961 gtcaggggca agccagtccg ggtaaaccag aggcagtccg gcagtgaacg aaccggaaac
    10021 atggccactg gtggtgctga gcccggcagc agcaccccac agcgtgccgg acgagtacag
    10081 gtcatctctg tcagagtgca gccagccgcc gtccagtgca gtcactgcac ggactgtccc
    10141 cacatatggc agggagaaca gagaccagga cagctcattt cgcagataac cgccgttatt
    10201 accggagata tactgctcct taaagccacg cactgaactc tcacccccga ggctcagttg
    10261 ttccacacca tgaagacggt ccggtgacca ctgggcataa gcgctggtca gccaccacac
    10321 cctgtccgtg acggggcgct gaaaactggc gcttaatgac catttccgga actgatttac
    10381 gggcaggtct ccccttttcc cgtggtcgct ttctgcgccg aaccagggca tcccccgtgt
    10441 gaataccgga ttcagtgttc cgacaccccc cagaaacttg tgtgtgtgat tcagcccgac
    10501 agaaaatgaa gtgagtttac ggctgctgcc ctgaagcaga acatcatcca gataattgtg
    10561 aataatgcgg tgctgcagac ctccggtcag tgctgtcttc atgtccccgt tacggaacag
    10621 gacatgcgac agtcccagcc ggtgagtctg caggtctccc gtggaacgcc accgccagcc
    10681 ccggttatca atggtgctga ggtagtcact ccatgaatac gtgtaatcca ccagggtata
    10741 gccatacggc agactgacac cggcggcaaa attcctcgca tcatgtgaca ccgaaaagtc
    10801 actgctccgt cccccgctga caaaccagtt gtcagccagc cccagcggat tattaaagga
    10861 aaggacacca tttaactgcc ccgtaccggt attcttctgt ccgctgttgt cgatgcccac
    10921 gctccctgtg acaggccatt ccggcgatgc cgtcagtgtc accaccgacc atccctcacg
    10981 gtcaccgggc gatatttaaa tctgtaccgg ctccgtacgc agacggttaa tctgctccat
    11041 cccctgctca atatcccgca ggttcagaac cttcccctcc attcccggga aaaccatctt
    11101 cagggtgcgg ccaggaaggt cagcgccttc cgcccggatt tgctgcagcc tgccttccat
    11161 gaccgttatg tgcagtacgc cccctgaaag gtcctgctct gtcagaaagg cccggctggt
    11221 gatatatccc cggcgtatat agccgtccgt cacggcatcc gtgaccgcgg tcagccccgt
    11281 gatattcaga cactgattca cccacggtgc caccagtctg tcggtttctg cagacgtcag
    11341 tcgggtggcc ccactgacaa caatgcgtga caccgtaaag cagggacctt cagtaccggc
    11401 agacatttcc ggagacggta tgacagtcag cggcgcactg cgctccagcg catcacgctg
    11461 acgctggttt tcatccagca actgctgctg ttgctgctga attgcactgc ggtcacccgg
    11521 agacagcatg gctgccggag acgaaaatgc ggggataaaa aaaccagaaa aaacggagag
    11581 ggagagataa catatccccg aagaggcgcg ggcgcgccgg aatatatcat aacaggggac
    11641 agacacagcc cggaataaaa gagacgtttt cgtcagaatg ttgttctgcc ggtgctgcat
    11701 gttctccccg tattctggcc tgtgtaatgt cagtgtaaat tcatgtcaaa aatgtgtcag
    11761 cgggaaattt acacctgaaa aagtctgtca tgaaacagct ttatgacaag atatcaaaaa
    11821 aaatgtcaaa aacgtgatct ttcttcagct tttcagaata ttgagagcct agaggtcaat
    11881 ataaattctg ggacacatat gcatttacca ttaaaaccgg cacaacaggc tgttcagtct
    11941 caatccatgc tttctgtttt atcgatctca gaatatcggc agacaaataa aaaaggaatg
    12001 tcgagagagc taactctaga tcactctcca cctcacaagc catttacaaa aatgatacag
    12061 gcatatcttt ggtccctgcc acaaccagac attgacaatc caatataacc ccaaatatat
    12121 ttcggatacg aagcttggga tttatacaga catttactat ttaattataa aatttgtatt
    12181 acatacataa gaggcactaa tgaaacgtta tataaaatgg cttgccatca caatttttat
    12241 cagtatgttg agtgcctgtg cccgtacggc tccagtgcaa cagataagca ccactgtcag
    12301 tgtgggtcat actcaggagc aggttaaaaa tgccattttg aaagcaggtg cacagcgcaa
    12361 gtggattatg acgcaagtgt cccctggagt tattaaagct cgctatcaaa cacgaaatca
    12421 cgttgctgag gttcgcatta catatacagc tacttactat aacatcaaat atgacaatag
    12481 cctgaatctg caggcttctg atggaaaaat tcataaaaac tataaccgtt gggtgcgtaa
    12541 cctggataaa gatatacagg ttaacttgtc tacaggagca acgttatagt gatgctgata
    12601 tcgggctgga atagcccgat atcattaaac ttctctataa gaatggttat atatgtacga
    12661 acaggacaca ctcagcacac tggatgccat cacggaggca caacggattg cttttgctcc
    12721 tatgcttttc caggcagctt tgtgcctgcg caatgcagga atattagatt atttggatca
    12781 acagggtaaa cagggagctc cccttaacgc gattactgaa cagacagtcc tgaatgaata
    12841 tgccgtgagt gtgttactgg atatggggtt aagcgggcga attattacct gtaaagaggg
    12901 aaattattac ctggcgaaaa tcggtcatta cctcctgcat gacactatga ctcgcgtgaa
    12961 tatggatttc acccaggatg tctgttacca ggggcttttc ttccttgccg attcactcaa
    13021 tgaaggaaaa ccttccgggt taaaagtatt tggcgactgg ccgacgatat atccggcact
    13081 ttcgcaatta ccagatgctg ctcgtgctag ctggtttgca tttgatcatt actattcaga
    13141 tggcgcattt aacgccgcac tgccttatgt attcgccaac aaccccacaa ccttgtacga
    13201 tgtgggcggc aatacgggta agtgggcttt gcgctgctgc aaatacaatg aaaacatcgc
    13261 cgttacgtta ttagatctgc cacaacaaat cgtactggcg aaagaaaata tcgcgaatgc
    13321 aggtttttct gatcgcatag atttccaagc ggtggatatg ctaagcgatg ccccgttacc
    13381 aggcgaggct gatatctggt ggatgagcca atttttagac tgtttttcac ccgaacaaat
    13441 aattagcata ctcagcaaaa tcgccagcgt aatgaagcct ggcgcaaaag tttgcatcat
    13501 ggagttgttc tgggatgcgc agcggtttga agcagcatcg tttagcctga acgcgtcctc
    13561 actttatttt acctgcatgg caaacggtaa cagccgtttc tatagtgcag agaaatttta
    13621 cgactatctg aataaagcag gattccaggt agcagagcgc cacgataatt taggcgttgg
    13681 acatacactt ttgatatgcc aaaagaaata atcgtcactt aataattttg gtttaccgcg
    13741 ggcacgcgtt gatgaaattt gcattaaata ttaccaactg gcaggcgctg gcgcctggac
    13801 ttagcgatgt gcagcaatgg caagcatggt cgcgccagcc gtgggcgatt gaccctgccg
    13861 cgccgctggc aaaattaagt gaactgccaa tgatgactgc ccgtcgtctc agttcaggca
    13921 gcaaactggc tgttgaatgc ggtctggcaa tgctacgccg ctatcagcct gacgccgttt
    13981 tgtataccag tcgtcatggc gaactggagc gtaattaccg cattgttcat gcactggcaa
    14041 ccgaacaggc gctttctccg acggacttcg cgctttccgt acacaattct tccgtgggta
    14101 acctgaccat tgcggccaaa cagccgatcg tttcgtcatc gctttcagct ggtcgcgaca
    14161 gtttccagca aggtttgtgt gaagtgctta gcctgctaca agcgggatac cagcgcgtgt
    14221 tgatggtcga ttttgacggt tttctgcccg agttttatca cccacaactg cctgcagaaa
    14281 tgccgacctg gccgtacgcc gtcgcgctga tgattgaatc tggcgatgac tggcaatgtg
    14341 aaacgcagtc agccattgcg gtaaatgaaa caacactgcc gcaaagcatg ttgtttttac
    14401 agcactattt acaaaatgca ggcgcatttt cactgtctgg cgagcgcgtg caatggcgct
    14461 ggagccgcag atgaataagc tgatgtcgcg cctgggatgg gcatggcgac tggtcatgac
    14521 cgggttatgt tttgccctgt ttggtctcgg cggcctgttg ctttcagtcg tatggtttaa
    14581 catattgtta gttttggtat gggatacttc ccgccgccgt cgtttggcgc gtcgcagcat
    14641 tgccgccagt tttcgcctgt ttttaactgt cgcgaaaggg cttggcgtgc tggattatcg
    14701 tattgatggc gctgaaattt tacgtcagga acgcggttgc ctcgtcgtag ccaatcatcc
    14761 cacgctgatc gattacgtgc tgctggcgtc agtcatgcca gaaactgact gcctggtgaa
    14821 aagcgcgctg ttaaaaaatc cttttcttgg cggcgtcgtg cgggcggcag attatttaat
    14881 caacagcgaa gctgaaaccc tgttaccccg ctgccaacag cgactggcac agggtgacac
    14941 tatcctgatt ttccccgaag gcacacgaac gaaacccggt gaaaaaatga cattacagcg
    15001 cggtgcagcg aatattgccg tgcgctgcgc cagcgattta cgcatcgtga caatcggctg
    15061 tagtgagcat ctgctggata aacaaagtaa atggtatgac gttccgcctg ccaggccgtt
    15121 ttttacggtt gaagtccatg ggcgggtaga aatcaataaa ttttacgatg caacttcaca
    15181 tgagccggcg ctggctgcaa ggcagttaaa ccgacatctt ttgcttcaat tacaacaagg
    15241 ttgtttacct ctctcaggaa taaataatgc aagcgcttta tctggaaatt aaaaatctca
    15301 tcatcagcac cctcaatctg gacgagttga ctcctgatga tattgatacc aatgcgccac
    15361 tgtttggcga cggtttaggg ctggactcta tcgatgcact ggaactgggg ctggcagtaa
    15421 aaaatgagta cggcgtcgtg ctctctgcgg aaagcgaaga aatgcgtcag cactttttct
    15481 ccgtcgccac tctggcctct ttcattgctg cgcaacgtgc ctgaggaaag ccatcatgac
    15541 agaccaacaa accatttatc aggaagtctc agccctgctg gttaagctgt ttgaaatcga
    15601 tccgcaggac atcaaacctg aagcgcgcct gtacgaagat ctggaactgg acagcatcga
    15661 cgccgttgac atgattgtgc acctgcaaaa gaaaaccggt aagaaaatca agccggaaga
    15721 gtttaaagca gtgcgtacag tccaggatgt tgtagaggct gtaaaacgcc tgctacaaga
    15781 aggctaattt gatgtcgggc attcgctcgc taccgatgat taagttgctg accggattat
    15841 tgctgctcgc ctggcctttt ttaatctggc tcggtttggc gcataacagc ctgcactggc
    15901 tgtcgccgct gatggcgttg ctgcttttat tgcgcctgcg ccagccccgt cgtcaggcag
    15961 ggccgctaca agccgtcaca aaaatcgtcg ctgttgtggg cattgtattg tgtgtctcca
    16021 gttttctgtt gaaaacgcac cagttactgc tgttttaccc ggtagtggtt aacgctgtca
    16081 tgctggcggt ttttggcggt tcgctgcggt cgacaatgcc gatagttgag cggctggcac
    16141 gtttgcagga accagacctc ccggaaaagg ccgtgcgata tacccgccgc gtaacgcagg
    16201 tctggtgcct gttttttatt ttcaacggca gtattgcgtt atttaccacc ttgtatggca
    16261 acatgccttt gtggacagcc tggaacggta tcattgctta ccttctgata ggtttcctga
    16321 ctgcagggga atggctgata cgttgtcaga tgatcaaacg gaaaacacca tgaatcagac
    16381 tctctcactt tcacagtggt tgacagcctc tcgccctgta acaactcctg ttgcctggct
    16441 tggggaatat acctggacac tggggcatct gcggcatgat gtagcgcttt tgatcgacca
    16501 tttacgcgac cagccaggca atcgctgggc actttgtttt gaaaacagct atctttttat
    16561 tgtagcgttg ttggccacgc tacactcagg taaaatccca gtaatcccgg gccataatcg
    16621 cgccgctttg ttaaatgtac aacgctcctt gtttgattgc atcctgagtg ataaaaggct
    16681 gggctggaac ggtccacagt ttgttgtaag cacatctcac cagatgacga ccagcacgat
    16741 agcctttgat gatattgctt ctgacgcgtt tattgaactt ttcacatcgg gttcaaccgg
    16801 acaaccaaaa caaattgcaa aacctgttat cagccttgat caagaagcaa acatgctggc
    16861 aactcacttt gcagaccgac tactgggctg tcgttttgta gcatccgtga tgcctcagca
    16921 cctgtatggg ctgactttcc gtattttttt acctatggca cttggcctgc cattgcatgc
    16981 cgcaatgctc tggtattctg agcaacttgc cgcattaagc catacctacc gctatgcctt
    17041 tgtcagcagc ccggcgttcc ttaaacgact cgaccttcat ctgacgcctc cccccgtgga
    17101 aatgattctc tctgccggcg gtatgctacc atggcaggat gtagagaaaa cttccacctg
    17161 gctgaatatc tggcccgatg aaatctatgg tagtaccgaa acaggtattc tcgcatggcg
    17221 ttaccgtcaa caggataata tcccctggtt tactttttct gatgttcatc tttcacagga
    17281 aagtgagggg gttcgtgtct tttcgccact catccctgct gaaggattgg tgcttgacga
    17341 tatgttgcaa ttcaatgaaa acggtcagtt ccacctgatc ggacgacgtg gtcgagtggt
    17401 aaaaatagag gagaagcgga tatcactggt tgaaatcgaa caacggcttc tggcacttga
    17461 cggtattatg gatgtcgctg caataccatt aatacgcaat ggccgccagg ctatcggtgt
    17521 cgtggttgta ccaaataaag aggcccgtca aatatggcaa tgctccggag ggaaaactct
    17581 ggagttgtcc tggcgcaaag cactattacc atggcttgaa cctgttgcag tacctcgtta
    17641 ctggcgcatt attgatgaaa taccagtaaa caatatgaat aagcgtgtct atgcacaatt
    17701 acaggagtta tttcatgata cgccatgaaa ttgagcgcca tcaggttcaa ctgcaggaga
    17761 tggaaatcgt tctatacctt gacccgatgc tctactggtt taatggtcat tttgccgtac
    17821 agcctctgtt accaggcgtg gcccagttgg actgggtaat gcattatgct accacgctgc
    17881 ttgctccggg ctggcgtttt cgtagtatcc agaatgttaa atttctggcg ccacttatac
    17941 ctgagacgac tgttaccctg caattaacat ggcaggaaac aagtcaggta ctcactttct
    18001 gctatcagcg tcacgacggt gacgcccgcc acaccgccag tagcgggaag atccgcctat
    18061 gtcggtaaac ttttctccct gcgtgttgat cccctgctac aaccacggtg cgatgatgcc
    18121 gggcgtgctg gcgcgtctta agccatttaa tcttccctgt attgtggtgg atgacggcag
    18181 cgatgccgcc acgcaacagc aactggataa tctggttgcc gaacagcctg gcgtgacctt
    18241 aattcgcctg gcagaaaaca caggcaaagg cgcggcggta atgcgcggct tacaggcggc
    18301 ggcagacgcg ggcttcagcc atgcggtgca ggtggatgct gacggtcaac atgcgattga
    18361 agatatcccc aaactgctgg cactcgctga acaacaccct gcggcgctga tctccggcca
    18421 gccaatttac gatgactcca tcccccgctc acggctttac gggcgctggg tcacccacgt
    18481 ctgggtatgg atcgaaacgc tctccctgca actgaaagac agtatgtgcg gctttcgcgt
    18541 ttatccggtt gcgccaacgc tgcaactggc aaaatacgcc accatcggca agcggatgga
    18601 tttcgacact gaagtgatgg tgcgcctcta ctggcaggga aacacctgtt atttcgtgcc
    18661 aacccgcgtc acttatccgc aggacgggct ttcgcatttt gatgccctga aagataacgt
    18721 ccgcatctcg ctcatgcaca cgcgtctgtt tttcggcatg ttgccgcgta ttccttcact
    18781 actgatgcgc cgctcttcca gccactgggc gcggcagagt gaagtgaaag gattatgggg
    18841 aatgcgcctg atgctgctgg tctggcgtct gctgggaaga acggcgttta gcgcgctgct
    18901 ttacccggtg gtgggcgtct actggctcac tgctgctcgt gcgcgcaaag cgtcgcaaga
    18961 ctggctcgcc cgtgtacgac agcatcaacc acaggcggca aaactcaaca gctatcagca
    19021 ctttctacgt ttcggtaatg ccatgctcga caaaatcgcc agctggcgcg gcgagctaca
    19081 actggggcac gatgtgctgt ttgcgccagg cgcagaagcg gcgcttgacg tccgcgatcc
    19141 gcgcggcaaa ttgctgctgg cctcgcatct tggcgatgtg gaagtgtgcc gggcgctggc
    19201 aaaaattcag ggctacaaaa ccattaacgc gctggtgttt agcgaaaacg cccaacgctt
    19261 taaacagata atgcaggaga tggctcccca ggcaggcatt aacctgatgc cggtaacaga
    19321 tatcggcccg gaaaccgcca tcctgctgaa agagaagctg gataacggcg aatgggtggc
    19381 gattgtcggt gaccgcatcg ccgtcacccc gcaacgcggc ggcgactggc gcgtctgctg
    19441 gagtccgttt atgggccagc ctgcgccttt cccacagggg ccgtttattc tcgcgtctat
    19501 tttgcgctgc ccggtgaatc tgattttcgc cctgcgccag cgcggcaagc tgcatattca
    19561 ctgcgaaacc tttgccgacc cgctgctgtt gccgcgtggc gaacgccaac aggggctgca
    19621 aaacgctatc gaccactacg ccgcgcgtct ggaacattac gcgctccagt cgcccctcga
    19681 ctggtttaat tttttcgatt tctggcaact gccggaaatt caggacaagg agtaaagggt
    19741 gcttaacgat ccccgattta ccgctgaagt cgagctgacc attccctttc acgacgtcga
    19801 tatgatgggc gtggcctggc acggtaacta ttttcgctac tttgaagtgg cccgcgaggc
    19861 gctgctcaac cagttcaatt acggctatcg gcagatgaaa gagtcaggtt atttgtggcc
    19921 agtggtcgat gcgcgggtga aatatcgcca cgccctcacc tttgagcaac gcatccgcgt
    19981 gcgtgcgcat atcgaagagt tcgaaaaccg tctgcggatt ggctatcaga ttttcgatgc
    20041 cgaaacgggc aaacgcgcca ccacaggata caccattcag gtcgcagtag aggagcaaag
    20101 ccgtgaactg tgctttgtca gccccgatat tctgtttgaa cgcatgggag tcaaaccatg
    20161 aaatttttac cgctgctggc gctgctgatt agcccgtttg tgagcgccct gaccctggac
    20221 gatcttcagc aacgctttac cgaacaaccg gtgatccgcg cccatttcga tcaaacccgg
    20281 acgattaaag atctgccgca gccgctgcga tctcagggtc agatgttgat cgcccgcgac
    20341 caggggctgt tgtgggatca aacttcacca ttccccatgc agctattgct cgacgataaa
    20401 cgcatggtgc aggtgatcaa cggccagccg ccgcaaatca tcacggcaga aaataacccg
    20461 cagatgttcc agtttaacca cctgctgcgc gcgctgttcc aggccgatcg caaagtgctg
    20521 gaacaaaact tccgcgtcga atttgccgat aaaggcgaag gccgctggac gctgcgcctg
    20581 acgccgacca ccacgccgct ggataaaatt ttcaacacca tcgatctcgc cgggcaaacc
    20641 tatctggaga gcattcagct taatgataaa cagggcgatc gcaccgatat cgctcttacc
    20701 caacatcaac tgacgccagc gcaactgagc gatgacgaac gccaacgttt tgccgcccag
    20761 taaacgcccc gcgctgttat gggggctagt ctgcctggtc atggcggtgg tattgctgat
    20821 cctgctgccg caatcacggc tgaacagtag cgtgctggct atgttaccca aacaggcgat
    20881 gggcgatatt cccccagcgc tgaatgacgg ctttatgcag cgtctggacc gccaactggt
    20941 gtggctggtc agccccggta aagaggctaa tcccagggtg gctcaggaat ggctgacgct
    21001 gctgcaaaag tccgctgcgc ttggcgacat taaagggcca atggatgccg ccagccagca
    21061 ggcgtggggg gcgtttttct ggcagcaccg caacggcctg attgaccccg acacccgcgc
    21121 ccgcctgcaa aacggcggcg aagcgcaggc acaatggatc ctctcccagc tttattctgc
    21181 gttctccggg gtaagcggca aggagttgca aaacgatccg ctgatgttaa tgcgcggctc
    21241 gcagctggcg atggcgaaaa acggccagcg tttacgactg atggacggct ggctggtgac
    21301 gcaggatccc cagggcaact actggtatct gctgcacggc gaactggcgg gatcgtcgtt
    21361 tgatatgcag caaacccacc agctcatcac gaccctgaat acgctggaaa aagatctgaa
    21421 aacgcgttac ccgcaggcgc agttgctctc gcgcggcacg gtgttttaca gcgattacgc
    21481 cagccaacag gcgaagcagg atatctccac cctgggcgtg gcaacgctgc tgggggtgat
    21541 attgatgatt gtggcggtgt tccgctcttt acgcccgttg ctgctttgcg tgatttccat
    21601 cggcatcggc gcgctggcgg gaacggccgc cacattgttg attttcggcg aattacacct
    21661 gatgacgctg gtgatgagca tgagcgttat cggcatttcc gctgactaca cgctctatta
    21721 cctcaccgaa cggatggtgc acggcaacga cgtttcgccg tggcaaagcc tggcgaaagt
    21781 gcgcaatgcc ttgctactgg cgctgctcac caccgtggcg gcatatctga ttatgatgct
    21841 cgcccctttt cccggtattc ggcaaatggc gatttttgcc gccgtcgggt tgagcgcctc
    21901 ctgtctgacc gtcctgttct ggcatccgtg gctgtgccgt ggcctgccgg tacgcccggt
    21961 tccggcgatg gcgctgatgc tgcgctggct ggcggcgtgg cggcgtaata aaaaactgtc
    22021 gctgggtctg cccgtcgcgc tggcgctgtt ttcgctggcg gggatgtcaa tgctacgcgt
    22081 cgatgacgat atctcgcagt tacaggcgct gccgcagcat attctggcgc aggagaaagc
    22141 cattaccgcc ctgaccggac aaagcgtcga tcaaaaatgg tttgtagttt acggcgactc
    22201 cccacagcaa accttgggac ggctggagac atttaccacc tcgctggaga aagcgaaaaa
    22261 agacggcctt atcagcaact accgcaccat tccgctgaac tccctggcgc ggcaggagga
    22321 agatttagac ctgctgaaaa cggctgcccc gacggtaaca aaagcactgc aaaatgccgg
    22381 gctgacggcg gtgaacccgg atcttaacgc catgccagtg aaggttgatg aatggctggc
    22441 aagccccgcc agtgaaggct ggcgtctgct gtggctgacg ctggaaaacg gcgaaagcgg
    22501 cgtactggtg ccggttgaag gggttaaaag tagcgcgttg cagcaggaaa tcgccagcta
    22561 ttaccctggc ggcattgcct gggttgatcg caaaagcacc tttgatgaat tgttcgcact
    22621 ttaccgttat gtgttaaccg gcttgttgct ggtggcgctg gcagtaattg cctgcggcgc
    22681 agtggcccgc ctcggctggc gcaaagggct tatcagcctg gtgccttcgg tgctttcgct
    22741 gggctgcggt ctggcagtgc tggcgatgag cgggcaggcg gtgaatctct tttcgctact
    22801 ggcgctggtg ctggtgcttg gcatcggcat caattacacg ctgtttttca gtaatccgcg
    22861 cggtacaccg ttaacttccc tactggcgat cacgctggca atgctcacca ccttgctgac
    22921 gctgggaatg ctggtattca gcgccaccca ggccatcagc agttttggta ttgtgctggt
    22981 gagcggtatt ttcaccgcct tcctgctttc gccgctggct atgcccgata aaaagagaac
    23041 aaaaaaatga tcaaatccac cttctggcga gcgctcgccc tgaccgctac gcttatcctc
    23101 accggctgta gccactcgca accggaacag gaaggccgcc cgcaggcgtg gctgcaacct
    23161 ggcacacgca tcacgctgcc tgcgccgggg atttcacccg ccgtcaattc ccagcaactc
    23221 ttgagcggca gcttcaacgg caaaacccag tcgctgctag tgatgcttaa cgccgacgac
    23281 cagaaaatca cccttgccgg gctgtcgtcg gtcggcattc gcctgtttct ggtgacctac
    23341 gatgcaaaag gtctacgcgc cgagcaatcc atcgtcgtcc cgcagctgcc gcccgcaagt
    23401 caggtgctgg ctgacgtaat gctcagccac tggccgatta gcgcctggca accgcaactt
    23461 cccactggct ggacgcttcg cgacaacggc aacaaacgcg agctgcgtaa cgccagcggc
    23521 aagctggtca cggaaatcat ctatctgaat cgccagggca aacgcgtgcc aatcagcatt
    23581 gagcagcatg tctttaaata ccacatcacc attcaatact taggtgactg atatgattta
    23641 tatttccgcc gtcggcatga tcaacgccct gggaaataat cttgacgaaa tcgcagccaa
    23701 cctggctcgc ggtgtcgccc ccggcatgcg tccgcgcgca ggctggttgc aggggcatcc
    23761 gcaggcggtc ctggctggcg tggatggcga gcttccgctt atccccgaaa aattcgctgc
    23821 gcaccgctcg cgcaacaatc agatcctgct ggcggcgctg gcgcagatcc agccgcaggt
    23881 tgatgacgct atcgcgaaat atggccgcga gcgcatcgcg attgtgcttg gcaccagcac
    23941 ctccggcctg catgagggcg acacgcacgt taacctgcgc accaacggac agccgagcac
    24001 cacatggcac tacgcgcaac aggaactcgg cgatccgtcg cgttttctca gccactggct
    24061 ggcgctcgac ggcccggcct ataccctttc aaccgcctgc tcttccagcg ccagagcgat
    24121 gatcagcggg cgcaggctta tcgaagcggg gctggtcgat gccgccattg tggggggcgc
    24181 agatactctg agccggatgc cgattaacgg ttttcacagc ctcgaatcgc tctcgccaac
    24241 cttatgtcag ccgtttggtc gtgaccgcgc aggtatcacc attggcgaag gggcgggact
    24301 gatgttatta acccgtgaac cgcagcccat cgcgttactg ggcgttggtg aatcgagcga
    24361 cgcttaccat atttcggccc cgcatccgca aggcgaaggg gcgatccgcg ccattaacca
    24421 ggcgctgacc gatgcgcagc ttacgccaga tgacatcggc tatatcaacc tgcacggtac
    24481 cgccacacaa ctcaacgacc agattgaatc aatggtggtt aacgccctgt ttggcaaacg
    24541 cataccgtgc agctccacca aacatttgac cggacacacg ctcggcgcgg ctggcattac
    24601 cgaggcggca attagtatgt tgattttaca gcgtgattta ccgctgcccg ctcaggactt
    24661 cagcctgtcg ccgcgcgatc ccacgctgcc accgtgtggc attatcgaaa aaccgcagcc
    24721 gctggcacgt ccggtgatcc tgtccaattc cttcgccttt ggcggcaata acgccagcat
    24781 tctgctcggg agggtttcgt gagccactat ttatcccccg gcgcttatct gccccacgac
    24841 gcgcccatgt tactgctgga agacgtggtg agcgtcagcg atgacagcgc ggtgtgccgc
    24901 gtgacggttt cgcccagtgg cgtactggca ccgtttctcg acccggacgg caatctccct
    24961 ggctggtttg cccttgaact gatggcgcaa accgtcggcg tctggtctgg ctggcatcgc
    25021 caccagcagg gcaaaaacag tattgagtta gggatgatac tgggcgcgcg tgaattgctt
    25081 tgcaccactg gcattctgcc cgcaggccag acgctgacca ttaccgtcaa actgctgatg
    25141 caggacgatc gcttcggcag ctttgagtgc agcatcaacg acggcgaggc gacgggccgc
    25201 attaacacct tccagccgac cgcggaagaa cttaccaccc tgtttcaaca aggagcgtcc
    25261 tgatgagtcg ttcagttctg gttactggtg ccagcaaagg cattggtcgc gccatcgcct
    25321 gcaagcttgc ggcagacggc tttaacattg gcgttcacta tcaccgtgac gccacaggcg
    25381 ctcaggaaac gctgaatgcc attgtcgcca acgggggtaa cgggcgtttg ctcagtttcg
    25441 acgtcgcaaa ccgcgaacag tgtcgggaag tgctggagca tgagatcgcg caacacggtg
    25501 cctggtacgg cgtggtcagt aacgccggga tcgcccgcga tgccgctttt ccggctttaa
    25561 gcgatgacga ctgggatgcg gtgatccaca ccaacctcga cagtttctac aacgtcattc
    25621 agccgtgcat tatgccaatg atcggcgcgc gccagggtgg gcgcatcatc acgctgtcct
    25681 cggtttccgg cgtaatgggc aatcgcggcc aggtgaatta cagcgcagcc aaagccggaa
    25741 ttatcggcgc aacaaaagca ctggctatcg aactcgcaaa gcgcaaaatc accgtcaatt
    25801 gcatcgcgcc agggcttatt gataccggga tgatcgaaat ggaagaaagc gctctgaaag
    25861 aggcaatgtc gatgatcccc atgaaacgta tgggccaggc ggaagaagtt gccgggcttg
    25921 ccagttattt aatgtctgat attgcgggtt acgtcacccg ccaggttatt tccatcaacg
    25981 gagggatgtt atgacacgtc gcgtagtgat tacaggaatg ggcggcgtga ctgcctttgg
    26041 tgaaaactgg caggacgttt ctgcacgctt actggcgtat gaaaatgcgg tgcgcaaaat
    26101 gccggagtgg caggtctacg atgggctgca caccctgctt ggcgcgcctg tggatgattt
    26161 cacgctaccg gagcactaca cccgcaagcg tatccgtgcg atgggccgcg tgtcgcaaat
    26221 gtcgacccgt gccagcgagc tggcgttaga gcaggcaggg ttgattggcg atccgatatt
    26281 aaccagcggc gaaacgggca ttgcgtacgg ttcatcgacc ggcagtaccg gtccggtgag
    26341 cgaatttgcc accatgctga ctgaaaagca cacgaacaac atcaccggca ccacctatgt
    26401 gcagatgatg ccgcacacca ccgctgttaa tactggcctc tttttcggcc tgcgcgggcg
    26461 ggtgatccca acctccagcg cctgtacctc cggcagccag gcgatcggtt atgcgtggga
    26521 agccattcgc cacggttatc aaacggtgat ggttgccggt ggtgccgaag agttatgccc
    26581 ttcagaagca gcggtgtttg ataccctttt tgccaccagc cagcacaatg atgcgccgaa
    26641 aaccacacct tcaccgttcg atgaaaaccg cgacggactg gtgattggtg aaggcgcagg
    26701 tacgctcatt ctcgaagaac tggagcatgc caaagcgcgc agcgcgacca tttacggtga
    26761 aatcgtcggc tttgcgacta actgcgacgc ggcacatatt acccagccgc agcgcgaaac
    26821 catgcagtat tgtatggagc aatcgctaaa aatcgcaggg ttaagcgcac aagatatcgg
    26881 ctatatttca gcgcatggta cggcgaccga tcgcggtgat atggcagaaa gtctggcgac
    26941 agcaaccatt tacggtgata atgtgccgct ctcctcgctg aaaagttatt ttggtcatac
    27001 gcttggtgcc tgtggcgcgc tcgaagcctg gatgagtctg caaatgatgc gcgaaggttg
    27061 gtttgcgcct acgctaaacc tgaataaacc agacccgaat tgtggcgcgc tggattacat
    27121 catgcatgaa gcccgcaagg ttgattgtga gtttttgcag agtaacaact ttgcctttgg
    27181 cggcattaat acatccatca ttattaagcg ctggccctga gttatacaac cacatggaca
    27241 tcccatgtgg tgattcttca aaaaatctga atcaatgaat atgaacctca aattctctta
    27301 cgcatactga agagcattcc gtataacgat tcagcagaca aaactttatc agtccacacg
    27361 aatcaacgga tgcacagatg acacgcccgg ttatcaccgg gcaatggttg ccataacggg
    27421 taacataagg gactgaacag ttgtctttag cgcaacgaac tgatctaacg tacctgaacg
    27481 gacaacctga aaacagcctt ccatcctgca ttgtccgcca gtgcttatta ccaaccacgt
    27541 ctgttgcagt aaagtaatca gttgcgaaca ttatttatca atcgtgacgc tggctcgcgc
    27601 atggcagaaa aggtcctgac tgagcaacaa caggcatcgt actgttgctg ctccatgttg
    27661 tttatatgaa cgcatcccgt ttgcaaggct tccgtgtttt tgacgttgac ggtagattgc
    27721 tgctctatat ccttattgca gccggtgctg cgggcctcaa tgagattcgc tgactctcgc
    27781 cttattcacg caacgagctt taagctcaga aaaacatcca agtttggtga gtcacagtct
    27841 tacctgtcgt tgtcatcaat ataggaatgg ataatctgga gagcgatact gcaggctgat
    27901 attgcggttt tgcgacaagt ttttagcatc cgcttctacg ccaaatacct gaagtaggaa
    27961 tggatgcgtt agcagcatac atccggaact gcgcggggaa tagccggaaa cggctatccc
    28021 taccacaata gctaactttg taatagcgct cttttaggct catgtactct gacgtaattc
    28081 tatctggtag ttgaattgca cgatacgcga ctcttctgat tcatcatgat tattattcaa
    28141 tagtccaatc agtaacttac ctgtctctat ccccattttt ttgtagtcaa tacggatgga
    28201 tgtcaaagtt gggtgtgttt gatcgcaatt gtctgaaccg tcaagacaag ctacagcgat
    28261 atttccaggg atttttaaca aacgccgctg gcactcaaac attataccca gcgcaatctc
    28321 ttcgtggctg caaatcacag catccagctc tggttgtcgc agtaatatct cgtttaatgc
    28381 gtaacgcccc aattgcagac ttgcggcatc cggcatggtg ataacaagat cagtgttctg
    28441 gtaatggctc agcattgctt tatgccagcc gttcagttgc tgacgttgca ggcggttgtc
    28501 catatgcgca ccgataaatc cgatattctt ataaccctgc tccagcagat gttcggtcag
    28561 tttatgtgat gcttcaaaaa atgctgtttc gatggtaatt tgagccggac tgaaagggga
    28621 accgataacg ttaatggtag gtatatttgt tttctcgagt atctggtgag ttttatcagc
    28681 caactgagaa ccaaaaataa ctaacgccgc cgggttgcct tgaagaagcg tcatcagaat
    28741 ttcagcttct ttgagctgat tatactcatg acacccaagc agtagctgga attcattttt
    28801 attaagcacc tgctgtaatg actgcataaa gcgggagctg gctttatctg tcagggaagg
    28861 aaccagtacg gcgactgtat gactgtgacc agatgcaagg gcgccagcag ttctgttggg
    28921 aatataaccc aactcttcta cggcctgttc tatcttttcc ctcagcttgt cagaaacctg
    28981 ttccggtgtt ctcagcgcgc gggaaacggt catggttccg acacctgcaa agttagcgac
    29041 ctcctgcagc gtcacccgtc cggtaccacg gcgcttacgt gttttttcca tttcgactcc
    29101 atggataatt acaaccagct gttttcatta cttttatttc accattactc tttccagttt
    29161 ttgcccacgg tagcaaatat cctgttgcat tgtatatgca ggaaccgaat atagccaaag
    29221 catggcggca gagaaatgat agcgctatca caaagattgg tagcgttatc agatagcgct
    29281 atcaaatgcg acaatcatca cattaattaa tgcgtaaaat atctactctc ctgttaatta
    29341 tcatcgggag ttatcagtag catgttaaaa aatctattaa atacagaggt cgttcaggtc
    29401 gtggaacagg tcaaagactg gcgggaagcc gtggcgattt catgccgacc gttgattgaa
    29461 aacggctcta tcgaaccgcg ttatgttgat gcgatctatc actcgcacga cactatcggt
    29521 ccgtactatg tggttggccc tggtattgcg atgcctcatg cgcgcccgga agagggggct
    29581 aacaagctgt cattagcact ga
//
LOCUS       DAZGTJ010000065        29378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000065 DAZGTJ010000000
VERSION     DAZGTJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.065"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1028)
                     /locus_tag="ACHK8E_003367"
     CDS             complement(<1..1028)
                     /locus_tag="ACHK8E_003367"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP0345589.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKV"
     gene            complement(1239..2636)
                     /gene="ccp"
                     /locus_tag="ACHK8E_003368"
     CDS             complement(1239..2636)
                     /gene="ccp"
                     /locus_tag="ACHK8E_003368"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP0345590.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3041..4690
                     /locus_tag="ACHK8E_003369"
     CDS             3041..4690
                     /locus_tag="ACHK8E_003369"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0345591.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4741..5343)
                     /locus_tag="ACHK8E_003370"
     CDS             complement(4741..5343)
                     /locus_tag="ACHK8E_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP0345592.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5863..6762
                     /locus_tag="ACHK8E_003371"
     CDS             5863..6762
                     /locus_tag="ACHK8E_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0345593.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6811..7824
                     /gene="yhjD"
                     /locus_tag="ACHK8E_003372"
     CDS             6811..7824
                     /gene="yhjD"
                     /locus_tag="ACHK8E_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP0345594.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            8217..9539
                     /locus_tag="ACHK8E_003373"
     CDS             8217..9539
                     /locus_tag="ACHK8E_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345595.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9718..11778)
                     /locus_tag="ACHK8E_003374"
     CDS             complement(9718..11778)
                     /locus_tag="ACHK8E_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0345596.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11848..12615)
                     /gene="pdeH"
                     /locus_tag="ACHK8E_003375"
     CDS             complement(11848..12615)
                     /gene="pdeH"
                     /locus_tag="ACHK8E_003375"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP0345597.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12847..13776
                     /gene="kdgK"
                     /locus_tag="ACHK8E_003376"
     CDS             12847..13776
                     /gene="kdgK"
                     /locus_tag="ACHK8E_003376"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP0345598.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13872..15368)
                     /locus_tag="ACHK8E_003377"
     CDS             complement(13872..15368)
                     /locus_tag="ACHK8E_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0345599.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15589..16875)
                     /gene="dctA"
                     /locus_tag="ACHK8E_003378"
     CDS             complement(15589..16875)
                     /gene="dctA"
                     /locus_tag="ACHK8E_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP0345600.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(17058..19046)
                     /gene="hmsP"
                     /locus_tag="ACHK8E_003379"
     CDS             complement(17058..19046)
                     /gene="hmsP"
                     /locus_tag="ACHK8E_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP0345601.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(19128..22619)
                     /gene="bcsC"
                     /locus_tag="ACHK8E_003380"
     CDS             complement(19128..22619)
                     /gene="bcsC"
                     /locus_tag="ACHK8E_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP0345602.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            complement(22601..23707)
                     /gene="bcsZ"
                     /locus_tag="ACHK8E_003381"
     CDS             complement(22601..23707)
                     /gene="bcsZ"
                     /locus_tag="ACHK8E_003381"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP0345603.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23714..26053)
                     /gene="bcsB"
                     /locus_tag="ACHK8E_003382"
     CDS             complement(23714..26053)
                     /gene="bcsB"
                     /locus_tag="ACHK8E_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP0345604.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(26064..28682)
                     /gene="bcsA"
                     /locus_tag="ACHK8E_003383"
     CDS             complement(26064..28682)
                     /gene="bcsA"
                     /locus_tag="ACHK8E_003383"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP0345605.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28679..>29378)
                     /gene="bcsQ"
                     /locus_tag="ACHK8E_003384"
     CDS             complement(28679..>29378)
                     /gene="bcsQ"
                     /locus_tag="ACHK8E_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP0345606.1"
                     /translation="TAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAM
                     LDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICTGLQQLKASGRYQW
                     ILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQ
                     VQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCL
                     LNYSGLKTPVGSAS"
BASE COUNT         6925 a         8090 c         7407 g         6956 t
ORIGIN      
        1 tgtactttgg tatagccttc acgaccgagg cgcaggaatt catagtactg tgcaatcacc
       61 tgacccgccg ggcgggagaa gttgatggcg aaagtaccaa tctgaccgcc gaggtagtca
      121 acgttgaaca ccagttcctg cggcagcgct tcttcatcac gccagataac ccagccgcag
      181 cccagcggag ccagaccgaa tttatggcct gaagcactga tcgatttcac acgcggcagg
      241 cggaagtccc agacgatatc cggggcgacg aacggtgcca ggaagccacc gctggcggcg
      301 tcgatgtgca tgtcgatgtc gataccggtg tcggcctgga atttatccag cgcatcgtgc
      361 agcggctgcg ggaactcata gttaccggtg taggtcacgc cgaaagtcgg caccacgccg
      421 atggtatttt cgtcgcaggc ttcaatcatg cgtttcgggt ccataaacaa ctgaccgggg
      481 cgcataggga tctcacgcaa ctccacatcc cagtagcggg cgaatttatg ccagcagatt
      541 tgtaccggac cgcacaccag gtttggttta tccgttggtt tgcctgcggc ttccatacgc
      601 ttgcgccaac gccatttcat cgccatcccg ccgagcatac aggcctcgga agaaccaatg
      661 gtgttggtgc caacggcctg accatttttc ggtgcaggcg catgccacag atcggcaacc
      721 atgtttacgc aacgcaggtc gattgcagcg gattgcggat attcttcttt gtcgatccag
      781 tttttattga tcgacaaatc catcaattta tggacgtttt cgtcgtccca ggtctggcag
      841 aaagtggcca ggttctgacg agcgttgcca tcaagatata attcatcatt gataatctga
      901 aatgcaacat catcgcgcat ttcgtgcagc ggaaatcgtt ttgactccgc gatagtagaa
      961 atggcctttg caccaaaacg tgaatcgagt agttctgagc ggaaatccgt taacagcttc
     1021 tggtccattt cgaactcctt aaatttattt gaaggcaata aaaaagtagg atttatccgc
     1081 aatggaagca aggcattaca ggcttaattt aaataacaaa atcctaagca gaaaaacgac
     1141 cttttataat ataaacattt aatatgataa tattaaaaac agactttatt atcgtaataa
     1201 aaatcgccca aacactcctg ttcgggcgat caaattaatt attgtttatc ctgcatatac
     1261 ggcgtgtaaa ccccgttcag actgtgcagg aaagctacga tatcatccac atcctcctgc
     1321 ggcagctctt tgcctacctg atagcgcagc atcagtttca ccgccccgtc cagcgtcggc
     1381 acgtcaccgc gatggaagta cggtgccgtt aaagcaacgt tacgtaaacc gggtactttc
     1441 tgacgcaatt tatcacgctc ttctttagta acattcatac gaccaatatc cgccgccgta
     1501 atttcaccaa agttaaagtc ttttttcagc cccaacggtt caaaggaacg tccgccgaga
     1561 ataataccac catgacaagt tgcacattta ttatctttaa ataattgata gccttttttc
     1621 tgttgcgccg tcagagcatt ctcatctcca cgcaaccatt tatcaaatgg ggaatccggc
     1681 gtaattaatg ttttctcaaa ttcagcaatg gcatcagtaa tattttcgcc actgaaacct
     1741 tgtggataga cttcgaggaa ctgcgcttta agctgcggat ctttttccag cttagcaata
     1801 atttcgtccc aggatttcga cgccatttca atcgggttca acggcggtcc accagcctga
     1861 tcctgcaatg ttgccgcacg accatcccag aactgctcaa cgttaaatac tgagttaaat
     1921 accgtcggcg cgttaatcgg cccaactgcg ccaccaacac caatcgatgt ttttctgcca
     1981 tcgacgcccc ccgcattcaa cgcatggcaa tgagcgcatg aaatggtgct atcagccgat
     2041 aaacggggat cgtgatacag cgcaaaaccc aacgccactt tttgcgcatc ggtaggcagt
     2101 ttttgcggga tgggctgcac cggttcattg cgatgctccg gagcagtatc attgctggcg
     2161 taatattccg cgcgctgttt tgcaatccag gccagtattt ccgcccgctc ttcatcactc
     2221 accttacccg cccagtgtag cgcggtataa cgcgttggcg gcatagtttc atactgcatc
     2281 acccattcaa tcttattcag atcgctttgc gaaacgggtt tattagccag cagagccgca
     2341 cgcacggcct cgaggttaaa agatttatat ccaagcttta tgtcgtaatc catcaactgt
     2401 ttcgcgccgg gaatataata ataggcgggt aattctgccg aaggtgtatg gcaatagtcg
     2461 catccttttt cacggagaaa gcctaaaacc ttattatttt cactgacagc agatgcctga
     2521 acatcggcct gtttactgcg tttattatcg tggtaccaca cataaccaga taaccctaaa
     2581 tagcaaatcg cgacgccagc caggccgatc gcggtaatac gtgagaccat tttcattatt
     2641 cttccctcac ggtggtgagt tattgtgacc cagaaaattt tccagaatgc tgcaaacgtc
     2701 tctgcgagac aaagccatca taaaaaacag ggagatatgc tttttgatag gaatcaatca
     2761 attacatgaa tataaactat caataagata gccagtgcct attacccaga tcaggaatgg
     2821 catatcatga ttgcggcgag agcaggattg aatgttctga aaatgaaaaa caacacgaaa
     2881 tccgtatttt tataaattcc attgatatta gtgcgttata ttttttggca ggttaataaa
     2941 cgtgatccac cgcacgcttt gtcgcccacc aggcggagcg aatgactacc cttaaagaaa
     3001 agcccgataa ttagcgacga atttcggagg tcggatcctt atgctcaatc agaaaattca
     3061 aaaccctaat ccagacgaac tgatgatcga ggtcgatctc tgctatgaac tggacccata
     3121 tgaattaaaa ctggatgaga tgatcgaggc agaaccggaa cccgagatga ttgaagggct
     3181 gcccgcctct gatgcgctga cgcctgccga tcgctatctc gaactgttcg aacatgttca
     3241 gtcggcgaaa attttccccg acagtaaaac ctttcccgac tgcgcaccta aaatggaccc
     3301 gctggatatc ttaatccgct accgtaaagt gcgccgtcat cgtgattttg acttgcgcaa
     3361 gtttgttgaa aaccacttct ggctgccgga ggtctactcc agcgagtatg tatcggaccc
     3421 gcaaaattcc ctgaaagagc atatcgacca gctgtggccg gtgctaaccc gcgaaccgca
     3481 ggatcacatt ccgtggtctt ctctgctggc gctgccgcag tcatatattg tcccgggcgg
     3541 ccgttttagc gaaacctact attgggattc ctatttcacc atgctggggc tggcggaaag
     3601 tggtcgggaa gatttgctga aatgcatggc cgataacttc gcctggatga tcgaaaacta
     3661 cggtcacatc cccaacggca accgcaccta ttatttgagc cgctcgcaac caccggtttt
     3721 tgcgctgatg gtggagttgt ttgaagaaga tggtgtacgc ggtgcgcgcc gctatctcga
     3781 ccacctgaaa atggaatatg ccttctggat ggacggtgca gaatcgttga tccctaatca
     3841 ggcctatcgc catgttgtgc ggatgccgga cggatcgctg ctcaaccgtt actgggacga
     3901 tcgcgacaca ccgcgtgacg aatcctggct tgaggacgtt gaaaccgcga aacattctgg
     3961 tcgcccgccc aacgaggtgt accgcgattt acgcgcgggg gcggcctccg gttgggatta
     4021 ctcttcccgc tggctgcgtg atactggtcg tctggcgagc attcgtacca cccagttcat
     4081 ccccatcgat ctgaatgcct tcctgtttaa actggagagc gccatcgcca acatctcggc
     4141 gctgaaaggc gagaaagaga cagaagcact gttccgccag aaggcctgtg cccgtcgcga
     4201 tgcggtaaac cgttacctct gggatgatga aaacggtatc taccgcgatt acgactggcg
     4261 acgcgagcaa ctggcgctgt tttccgctgc cgccattgtg ccgctctatg tcggtatggc
     4321 gaaccatgaa caggccgatc gtctggcaaa cgccgtgcgc agccgattac tgacacctgg
     4381 cgggattctg gcaagcgagt acgaaaccgg tgaacagtgg gataaaccca acggctgggc
     4441 accgttacaa tggatggcga ttcagggatt taaaatgtat ggcgatgacc ttctgggtga
     4501 tgaaatcgcg cgcagctggc tgaagacggt gaatcagttc tatctggaac agcacaaact
     4561 gatcgagaaa taccatattg ccgatggtgt tccccgcgaa ggcggcggtg gcgagtatcc
     4621 gttgcaggat gggtttggct ggactaacgg tgtggtgcgc cgtttaattg gtttgtacgg
     4681 tgaaccataa tatttttaca gccagccgct aacttcctgc tggctgtaaa attatcctct
     4741 tcaggaggag atatttaaca tcattgccgc ctgggtgcga tttttcactt ccagacggcg
     4801 atacagggat tccagatgcg cttttaccgt tccggtactg atattcagcg ccctgccgat
     4861 ctccttattt gattcgcccg ccgctaacat ggttaaaatc tcccgctggc gggcgcttaa
     4921 cgatttgaga tctttaatgt ccttttccgg cgtcgtccgc cagtctccag gcagaaacat
     4981 catccccatc gccgcactat ttaccgccaa cgcaaatgtc tcgacggttg aatcacgagg
     5041 cacaatggcc agcacattaa aatggataac ttcctgtaac caccgtttat tgcaatccgt
     5101 cgccgtaatt aacaccttaa cctcaggaaa ttgcaccacg gttttttgca gcaaccagta
     5161 acaaaactca ccatcctgat cgccatcgag catcactaag gcttcagggt aactttccag
     5221 cttttgccat aactcgtctg cctgactggc cccctgaata ctcactcctg gaatacgttg
     5281 ctgtaaactg attttcattc catgaataaa tattgactgc ctgtcaaaca tgactatttg
     5341 cataactgaa tctccacctg aatacgttaa aaagacttaa gtagtggaag ggtattaccc
     5401 gcgagaaaaa ataagaattc gccatttggc ggtggccatt ctacagagat gacgcgtaga
     5461 aaatagttac cgatataaat agttacagct aaacgcctga aattacatgt cgagggcact
     5521 atttaaaaca attttgagga tttccttata ttagtggtta gtacgcatgc aattaaaaat
     5581 gaaattccgc gaccacaagc caaaataaca aatggcaatg agacaaaaat aagcacaaat
     5641 agccaatacg tcctctattc actttaaagg gaatcgctga aaaatacgct ctgtttaagg
     5701 ggattcacct ttctaagaaa gctattcctc ccttttccta ctgagaaatc gccacattcg
     5761 tcatgacaac attgtgaaac ccggctttag atgttagaaa aaacaataaa caatgcgata
     5821 tgcgcgttac tgtttcgctg tgaacaacaa tcggtcaaag aaatggataa aattcacgca
     5881 atgcagttgt tcatcaaagt cgcggagctg gaaagttttt cccgcgcggc ggatttcttt
     5941 gctttgccaa agggaagtgt ttcgcgccag atacaggcac tggaacatca acttggcacc
     6001 cagcttctcc agcgcactac gcgacgggtc aaactcacgc cagaaggcat gacctattat
     6061 caacgggcaa aagatgtgtt gagtaatctc agcgaactgg acggtctgtt tcaacaggat
     6121 gccaccagta tcagcggtaa attacgcatc gacatcccgc caggaatcgc gaaaagcctg
     6181 ttactgccgc gcttgtcgga atttctctat ctgcatccgg gaattgagct ggaactgagt
     6241 agccatgacc gtccggtaga tattcttcat gatggttttg attgcgtgat acgcactggc
     6301 gcattaccgg aagatggcgt tatcgcccgt tctctcggca aactgaccat ggtcaactgt
     6361 gccagtccac actatctgac gcgctttggt tatccgcaaa gccccgatga tctgacttca
     6421 cacgcaatag tgcgttatac accgcacctg ggtgtacatc cgttaggttt tgaggttgcc
     6481 agcgttaatg gcgtccagtg gtttaagtct ggcggcatgt tgacggtaaa cagtcgcgaa
     6541 aactatctcg ccgccggtct tgccggtctg gggattattc agatcccgcg cattgccgtg
     6601 cgcgaagccc tgcgtgccgg gcggcttatt gaagtgttgc ctggctaccg tgccgagccg
     6661 ctctcccttt cactggttta tccgcagcgt cgggagcttt cccggcgtgt aaacctgttt
     6721 atgcagtggc tggctggcgt aatgaaagag cacctggact gaccgactat actttttaag
     6781 aatgaccacg acagaaggac aaaagagcgg atgacgcagg aaaacgagat caaacgtccc
     6841 acccaggatc tggagcacga gccgattaag cagttggata atagcgagaa aggcggcaaa
     6901 gttagccagg cgctggaaac cgtcaccact actgccgaga aagtccaacg ccaaccggtt
     6961 attgcgcacc tgattcgcgc aacagagcgc tttaacgatc ggctgggtaa ccagtttggt
     7021 gcggctatca cctatttctc atttttgtcg atgatcccga ttttgatggt atcgtttgcc
     7081 gcagggggct ttgtgctggc ctcccatccg atgttgctac aggatatctt cgacaaaatt
     7141 ctgcaaaaca tcagcgatcc gacgctagcc gccacgttga aaaacaccat caacaccgcc
     7201 gttcagcagc gtactaccgt agggcttgtc ggcctggcgg tggcgcttta ttccggcatc
     7261 aactggatgg gtaacctgcg tgaagcgatt cgcgcccagt cacgcgatgt ctgggaacgc
     7321 tcgccgcaag atcaggagaa gttctgggta aaatatctgc gtgattttat ttcgctgatt
     7381 ggtttgttga ttgcgctgat tgtgacgctt tcgatcacct cggttgccgg ttcggcgcag
     7441 caaatgatta ttagcgccct gcacctgaac agcattgagt ggctgaaacc gacgtggcgg
     7501 ttgattggcc tggcgatttc catcttcgcc aactatctgc ttttcttttg gatcttctgg
     7561 cgcttgccgc gccaccgtcc gcgcaaaaaa gccctgattc gcggaacatt tctggctgcg
     7621 attggttttg aagtgattaa aatcgtgatg acctacaccc tgccatcgct gatgaaatcc
     7681 ccctctggcg cagcatttgg ttccgtgctg ggactgatgg catttttcta cttcttcgcc
     7741 cgtttgacgc tgttttgcgc ggcgtggatt gccaccgccg aatataaaga cgacccgcga
     7801 atgccgggga aaacgcagcc ttaaaatagg ccggatgcgg cgtacgaatt tattcggcct
     7861 ggctccccgt aggccggata agatgcgcca gcatcgcatc cggctataat gcgcacataa
     7921 cctcttgaaa ctctttccca gagcctcttt agccatctat tcaggagcaa acaatttcat
     7981 accaactcat aaccccagca tataaatcca gttggtaact tttatttaac ctgaaaccag
     8041 ttttatccac tatttataaa attatgtgaa gcatttcata gaagaaaaat cactggccta
     8101 aacattatcc cctttttgcc tggtttttga ccatttccgc gatttgttac acattgaaat
     8161 atcacttttg ctgtgcgtaa tatggctatt cgttagccaa aaaataagaa aagattatgc
     8221 aagcaacagc cacaacactc gaccacgagc aagaatacac gccgatcaac tcgcgtaata
     8281 aagtccttgt cgcctctctc attggcacgg ccattgagtt cttcgacttt tacatttacg
     8341 ccactgcggc cgttattgtg tttccgcata tcttcttccc gcagggcgat cctacggcag
     8401 caacgctaca gtcgctcgcc acctttgcca tcgccttcgt cgcgcgcccc attggctctg
     8461 ccgtttttgg tcattttggc gatcgcgttg ggcgtaaagc gacgctggtc gcctcgttgc
     8521 taacgatggg gatttcgacc gtggtgattg gcctgctgcc aggctatgcc acgattggta
     8581 ttttcgcccc gctgctgctg gcgctggctc gatttggtca gggtctgggc ttaggtggtg
     8641 aatggggcgg cgcggctctg ctggcgactg aaaacgcccc accgcgcaaa cgtgcattgt
     8701 acggctcctt cccgcagctg ggcgcaccga ttggcttctt ctttgccaac ggcactttct
     8761 tgctgctttc ctggctactg accgacgagc agtttatgag ctggggctgg cgtgtgccat
     8821 ttatcttctc ggcggtgctg gtcattatcg gcctgtatgt tcgcgtgtcg ctgcatgagt
     8881 cgccggtgtt tgagaaagtc gctaaagcga aaaaacaggt gaagatcccg ctgggtacgc
     8941 tgctgaccaa acatgttcgc gtaaccgtac tgggtacgtt cattatgctg gcaacctata
     9001 cgctgtttta catcatgacg gtctactcta tgacctttag taccgccgcc gcgcccgttg
     9061 ggcttggcct gccgcgtaac gaagtgttgt ggatgctgat gatggcagtt attggttttg
     9121 gcgtgatggt gccagtcgcc ggattactgg ctgatgcctt tggtcgccgt aaaagcatgg
     9181 taatcatcac cacgctgatc atcctgttcg cgctgttcgc ctttaacccg ttgctcggtt
     9241 ctggcaaccc gattctggtt tttgccttcc tgctgctggg gttaagtctg atggggctga
     9301 ccttcggtcc gatgggcgcg ctgttaccag agctgttccc gacagaagtg cgttacaccg
     9361 gagcatcgtt ctcttacaac gtagcgtcga ttctcggggc ttccgttgcg ccgtatatcg
     9421 cagcctggtt gcagactaac tacgggttag gtgcggtggg gttatatctg gcggcgatgg
     9481 ctggcttgac gttaatcgcc ctgctgctga cccatgagac gcgacatcag tcgttgtaat
     9541 aagagtgaaa agcctgatgc gctacgctta tcaggccaga atttccatac aatatattga
     9601 attagcaagg atttgtaagc cggaggcgtt cacgccgcat ccggcatgaa caaagcacac
     9661 tttgccaaca atctaaatct gtttaattgc cggatgtcag acatccggca attatcatca
     9721 cttcttcatc tgcgacaaaa ttgtcctgca ctgattagcc tccccttcgg aaggtgagat
     9781 cagcgccagt aacgccgccg ccggtgttac cagcgttgcc agtgccgccg caacggctcc
     9841 gcgagcaatc aacggcccgg ctttcacccc agcctgcgga tttttaaacg tcccacgtac
     9901 atacagcggc gaacgcaatg tgataatccg aatcccttta ctctccggat caatagtcaa
     9961 atccagctgt tccgaagcaa aacttgccgt accggtaacg ttaatcaacg cgttctcagt
    10021 atcgaaagca aaaatctgcg ggcgcgccac gccgttggca atatccagat tcgccgccgc
    10081 acagttcacc cgcacctcat catcaccaaa tatcgcaccg acaatgtagt taccgacatt
    10141 cagcccgaca atctccatca ggttgcggct caccagcccg tcattcatca gcagtttcag
    10201 gttgccgtta ctgttgccta aaagcgccgc caccgagtta ccgctaccgc gtagttccgc
    10261 gtcaccgttc atttccccca gcgtcttctg catcagttct acatcgggca tcagttcttt
    10321 cagtttcagt cgacgagcct gaatatctgc ccgcccttgc atcggttttt tatcaccttc
    10381 cagatgaata ttcgccgcaa tgctgccgcc tgccatgcca aatttcagcg gttgcaggcg
    10441 caggtcagca tttttgagga tgatatgagt agaaagatcg ctaatcggca ggctactgcc
    10501 atgctcaatg cgccgccctt tgaagcgaac atcggcatcc atgacgtccc atttatcggt
    10561 ttcgaagcgg tcataaggca gcactttgcc cgcaggctga acgctttttt cacccttctt
    10621 ctgttcagac cgtttcgact tttcagctcc tttcccggaa tcaacgccaa ttaacggccc
    10681 taaatctgcc agccgcaatt gccgcgactc aacatcacct tccagttttg gtcgtggctt
    10741 gccggtggtg tagaccagag aaccgtggat atcgctatcg ccaattcgcc cattaaaacc
    10801 gcgataatca aagaccgacg atttttcagt gtcgattttc gctaccagcc gaccatccgt
    10861 ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt tcatagagat cacccagcga
    10921 atcgccagaa aatttaagcc gtaaatcgac accacccatc ttcattgggt cattcacaac
    10981 gccatcaaaa gcaacacggg tgttaccaga gcgaaaatca gcctgtacag gaaatggcgt
    11041 cccttcgcca cgcagcgcca gcataccgcc tatttttccc gtaccagtga gcggctctcc
    11101 gttataacgt ccctgcgcct tcaggccaaa aacgtaatcg cccacctttt ctttatccgc
    11161 tttacctttc gatccagtaa cttcgctgaa cggcagcggc ttgcctaacg gatcgacaaa
    11221 aatttctaga tccgctttgc ttactttgtc atcaatggcg atccgccctt gatcgaacag
    11281 aatattatcc agccgaaacg accatgccga cggctttgca ttcgtgtctt tgttgtcatc
    11341 gctggcgaga ttaaatgtcc agttgttgtt cttttctgag aggcgaatca ggcgtgcgtc
    11401 gggcttttcg agcttgatcc acggcaacca gaccgttttg gtcagcagcg ccagcggggc
    11461 cagcgtcgct tctactcgcg gcaaatgcac cattgtgact tcgggaatat ccggtggatt
    11521 gccaagaatg atgtcttccg cgtgtacatg gggccacggc acccagctgc gccagccagt
    11581 ttcttgtttt tgccgctccc acaccacgcc cagatcgcca cggatagcga acggacgatt
    11641 caactccgca gagactttct ggttgatggt cggtttgagt cgattccagt caaaagttgc
    11701 aatcaaaatg atcgccacga caatcaacaa caagaaagcc cctgaaatcg cagcggttat
    11761 tttgcctgcc ttgctcatcg ttcgcttcct catattcttc ctgtgccagt cctaaagata
    11821 gtccagccag gcggaaaatg aggcagctta tagcgccaga accgccgtat tcagcgtttc
    11881 tatcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg aatgcgggcg agttctgaac
    11941 atcacgccac tcttccggcg tttctacgcc ctcgacaatc accccgcgac aatagcgatt
    12001 catgagatgt aaaagctgag aaaagagtgt gcggccttcc ggcgactgac gcagcatcac
    12061 gaacagctct cgcgcgattt tgatgtagtc ataacgcact tcacttagcg cagagaaatt
    12121 tgccatcccg gtgccaaaat catccagcca cagcgggcca aattcacaca tcgaggcaaa
    12181 ggttgaatct ttcggcagac ggatatgctc caccagttcg aaacgcagcc agggaagacg
    12241 ctcaatttgg cgcaggattt ttggttgctg acgcagggcg atgagcgtag ggccatcaat
    12301 attgaccgat gccagcaggt cgtgctctat aaagaagtcg gctttttgcg ccagcaaatc
    12361 aatctgctct ttcacaacct ccatccgatg gctaacggtg atttcagtaa aatagcgatc
    12421 cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc accgttaata gctccacggc
    12481 cattaaccgt ccgcatgttt gatagatcgg ctgccaggtg taagcacgct cacactgcaa
    12541 ccaaaaacgc cgttcctgca agctctcgat gcttgcttca gggttgctta ttcgctggat
    12601 aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa
    12661 agattatcgg cgcgcaaggg cagaacttta ccgcctggtc ggggtaaatt tccccactcc
    12721 acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa taatggaaca ctgttttaat
    12781 atggttgacc agcaaaccac cacagcgcaa actaacgcta attttttaca gatcaggttc
    12841 acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa
    12901 ggcgcggacg ttaagcgcgg tttcggcggc gataccctga acacttccgt ctatatcgcc
    12961 cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa cggcgctggg aacggacagt
    13021 tttagccagc agatgctgga cgcctggcac ggcgagaacg ttgatacttc cctgacccaa
    13081 cggatggaaa accgtctgcc gggcctttac tacattgaaa ccgacagcac cggcgaacgt
    13141 acgttctact actggcgtaa cgaagccgcc gccaaattct ggctggagag tgagcaatcc
    13201 gcggcgattt gcgaagagct ggcgaatttc gattatctct acctgagcgg gattagcctg
    13261 gcgatcttaa gccagaccag ccgcgaaaag ctgctttccc tgctgcgcga atgccgcgcc
    13321 aacggcggaa aagtgatttt cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa
    13381 gagacacagc aggtgtacca acaaatgctg gaatgcacgg atatcgcctt cctgacgctg
    13441 gacgatgaag acgcgctgtg gggtcaacag ccggtggaag acgtcattgc gcgcacccat
    13501 aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt
    13561 gctggcgaag ggttagtgga tgttccggcg gtaaaactgc cgaaagaaaa agtgatcgat
    13621 accaccgcag ctggcgactc tttcagtgcc ggttatctgg cggtacgttt gacgggcggc
    13681 agtgcggaag acgcagcgaa acgcgggcac ctgaccgcaa gtaccgttat tcagtatcgc
    13741 ggcgcgatta tcccgcgtga agcgatgcca gcgtaaaagc agcatatgca aaaggctgga
    13801 tagagtgtta cccccctatc cagctcatcc ataaatacct gatggttgtg ctaactcatc
    13861 accatcaggc attactgtac aggtggaata tccgtcactt caggatgtac gtcatccgtg
    13921 gcgacagatg tggcggcagc cgcagtcgat ggggccatga tttgatccca ggccgcctgc
    13981 aacgctttca tgttaaattc cggctcgcct ttcggctgca gcagtattaa cgccatatca
    14041 ttcgacaact gctgacgcag atcctgattt aacatctcca ccgtcaggct gttaaggaaa
    14101 tcctgacgca gtttctgata ttgctccggc gcgatatcga cgacctgatt ttgcaacgaa
    14161 cgcatccgct gccccatcag aatatcggta tcagctcgcg cataggcggc aaacagtttc
    14221 tgcagctcca gtttcttttg cgccactaac gcattgaact cttcttccgg cagaccttta
    14281 tcgcgaacct tcgccagttc acgcgccacc agattaaggt tgctgttcag cttgtcgttt
    14341 ggtgattcga tgttgatggc acactgcgca cgcagataca gcacacgaca gtcaaatcca
    14401 agaccgatgt ctttactgtt actggcgctt aacgcttgct gaacatgcca gaacagcgcc
    14461 tcacgggcca ggtccgcacg ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc
    14521 cacggcgtat cccacatgat tgataaccgg tcctgacgca ccgcgtcagt cataatactc
    14581 accgcttccg cacgcagcgg agaaagcgtc ggcaccggag ccggtgtttc acgtttgcct
    14641 ttcagttcgc caaacgtttt attgatttgg tcgacgaccg agcgcgcatc cacgtttccc
    14701 accaccagca gcgtcattgc atccggggta taccatttct gatagaaatc tttaatcttt
    14761 tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt gacctaacaa ggttgatcct
    14821 ttcagacgat agcgccacca gccctcttta gtatcggcag gccaggttgc caccatgtcc
    14881 tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg tcagtttgcc agtggcattt
    14941 gccagataag agagcgcttc tttcagcaag tcgttacggt tattgggcaa actcagatta
    15001 aacagcgtgg tgtcataaga gacaattact ggcggcatcg ggcgtttagg gtcgatcccc
    15061 tgctgccaca atgaacgcgc ctgtgctgct tcaaggccac cgctttgcgt taacgcaata
    15121 cgagggatgg cgtggctgta accgctctgt tgtgtacttt cggcgagcga accggtatta
    15181 accagcaggc gaatttcaac acgatcgcta ggacgctggg gcgtggtcag cacttgccac
    15241 tgtaaaccgt tggaaagcgt cccctgttgc catgctggat caggctggag cgcatctgcc
    15301 tgcacatagc cagcagtggc catcatcagc aaaccgcccg ctaaaagtcg aatttttgtg
    15361 ccctgcatgt gaacccctga tcaactatcc tggtaataaa aaaactgccc gttgtcggtc
    15421 agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa atgtcacgga
    15481 agaagtgaaa taaacctgaa ctcacccagg tggtaagttc aggcacaggg gtcaattatg
    15541 cgcaaacacc cgcactcggg gaagggagtg cgggcataag tgatgagatt aagaggataa
    15601 ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca tcgtccagtt ttttgtggtc
    15661 cagttctttc acccacttag caacgacaat ggtcgctacg ccgttaccga ccaggttagt
    15721 cagcgcacga gcttctgaca taaagcggtc gataccaagg atcagcgcca gacctgctac
    15781 cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg ataaagccac tgcccgttac
    15841 cccggcagcc cctttggaag agagcaacaa cacaattaat agcgtaattt ggtggacgat
    15901 atccatctga ctgttagtcg cctgggcgat aaacaccgcc gccattgtca ggtatatcga
    15961 tgtgccatca aggttaaacg agtagcctgt cgggatgacc agccccacca ccgatttccg
    16021 gcagccgagt ttctccatct tgtcgagcat acgcggcagc gccgactcgg aagaagaagt
    16081 gcccagtaca atcagcagct cttcacggat gtagcggata aatttgaaga tactgaaacc
    16141 agtcgcttta gcgattgaac ccaataccag caccacaaac aggatacagg tgatgtagaa
    16201 acagataatc agctgcccca gttgcaccag tgtgccgacg ccgtatttac cgatggtaaa
    16261 cgccattgcc ccgaacgcac caataggtgc cagacgcatg atcatattga tgatgccgaa
    16321 gatgacctgc gagaaacttt cgatgacgtt aaaaatcagt tggcctttgc tgcccagacg
    16381 gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt taccgctggc
    16441 aaatgcgcca atgacgctcg ccgggatgac atccataatg aaggcgacaa tgccctggtc
    16501 tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg gatcgacgtt
    16561 cattccggca ccaggctgca cgacgttaac gatgataaga ccaataatca gcgcgatggt
    16621 actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta cgaccaaccg ccttcatgct
    16681 ttccatgccc gcaatgcccg ttacgacggt acaaaagatg acaggagcga tgatcatctt
    16741 aatgagctta acgaacccgt cgccaagcgg tttcatttgc tcgcctattt cagggtagaa
    16801 atggccaagg agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa ggcttttaaa
    16861 cagagaggtt ttcatagggt gtcctttagt aaaaccacag gtcttgtaag gttatggggt
    16921 acctgcggcc ctaaaataac acccagacaa catcacagaa atgtacctgg atcataattg
    16981 aaacaaaaag gttaaaaagt ttgagctggc tcgcacaaac cagcactttt taaagttttg
    17041 taatcagttt ggggtagcta cttttcttcc aggtaactct cttcgaagat ttcaataggg
    17101 agtgggcgag caaaaaggaa gccctgggca ataccaacgc ccgcttttgc cagccagtcg
    17161 cgttgtgctt cagtctccac gccttcggca atcatttgta agttcaggct ttgcgccaac
    17221 atgataattg cagcaatcat gctgctatct tccggcaagc cttcaacaaa cattttgtcg
    17281 attttcagta cgtcgattgg caacgatttc atatgctgca gctgacgcag ccctgcgtag
    17341 cccatgccga aatcatccag cgccacccga actccggcat tgcgcagcgg acggaggatt
    17401 gccaccgcag catgagggtc gtcaatacgt cggctttctg tcacttccag aatcagtgtt
    17461 cccggctgaa tgcgatagcg ggttaacagt tccagcatat ccgctaccat attcgggtgc
    17521 atcagttgta gcgctgagag gtttaccgac aagggcagca taatgccgcg ctcttgccag
    17581 gcagcaagca gtcgacaaga ctcttccagc acccagtgac caacggtaac catcagccca
    17641 cagcactcaa tgcgatcgat taagccatcc ggcagatccc aactgccatc cggttgctgg
    17701 atacgcagta acacttccgc actgaccagt ttaccgctgg tcatctcgac ctgtggctgt
    17761 aaccaaatcg caaattgatg attttccagt gcattaagga tatcgctctc ttccgtcaac
    17821 cgctgctggg cggcttccat ctgctgcgga tcaaagaact gaatctgatt tttgccttta
    17881 tggcgagcgg taaatgccgc agaaatagcg cgactgtaaa gctgttcggc ggtgagatcg
    17941 ccgtagaaca tcgccacgcc aatgctacag tgcggacgga gttgaatacg ttcaatcggc
    18001 aggcgctcgc tcatgatagt gagcacttgc tgacctaatg tgatagcgtg ccacggttcc
    18061 tgtacaccgt tggcaatgac agcaaagtca taaccgctaa tctgcgcgag gatcatgcgt
    18121 ggcgacagta ccgatttgag tttttccacc agcgtcagca gcagaatttc tcgttgcgcc
    18181 tctttcagca cgcccgcagt atcacgcagg gtttcacagg tgatgatcat cagcgcggtg
    18241 gtttgtttac gcgcgacaac ctgctccagc atctccatca gcaaggcttt gttcggcaaa
    18301 tccgacaccg ggaagcgcat cgcgttttcg ttctgttctt cataatggcg ctgcagcaat
    18361 tgctggttga ggttgtaact gcgcaccagc ataccgattt catcgtcctg atgcagacgt
    18421 ggtaatgcca gttggtgacc aacaagctcc tgggctggga tggcgttaag ttcgcgagca
    18481 atattgcgta acggatgtaa aatcagtcga ttaatacacc agctgatggc gacggtcagg
    18541 ataagcgaca aaagtaagta aatggtcact aacgttgaga gggtgctcat cacgaactta
    18601 tacatacgga aggaatccgc ctgtagcacc agataggcaa ttggctgcgg atttgccgga
    18661 cgttcgagcg agtagacgcc cagcgagatt tgaaccggta gctcaaacag gcgagtaacc
    18721 attaccggca ccggacgctc tggaataaaa cttttacgca gcgcctggaa ctggttgggc
    18781 agcaccacat cggcacggct gaccacgcct gccggtttaa tgctggcaag aatagcttcc
    18841 gcttcgggaa tatcgccttt caaaatagct gaagataagg gttcgcggac agagcgagca
    18901 atgctttcca gttgcgtagc cgtgttatag cgattctgct ggaccagatg gaacagcaaa
    18961 acggtgcaaa aaataaaaac gaacaccagg acaacggctg ccaccattgc catctgcttg
    19021 attgttaacg agcgacttac gcgcaaattg tctccccccg gaaatcccag attgccgccc
    19081 gagtataccc gatcgcagcg gcattaagag aggcgctatc tgaaaactta ccagtcggcg
    19141 taaggtatca gcggctgcgg cggtaaatcc atgtcaccct gccatccggc ggcggaataa
    19201 cgtacgtaga gcaggaaatg gctgggtgcg taatctttcg cctgctggat atcaattgcc
    19261 gtgccgacaa accagttgga agtaacacgt cgttcaagta atgcccgcgc cgtatagccg
    19321 aagccctgac tgctgccgcc atcgttggat tgccgcgcag cttcttcctg ccagtcggtc
    19381 gggatcagat tcatcagcgg ataacgcggc atggttttgg tgcgtgaatg cgaccacgag
    19441 ccagacgccc ccagctccca cgaccagttt tccgtgcgct cccgccacat caccggtatg
    19501 gcaaacgaca ggtattcctg cgggctgtag taaccgccct gaccgagtga gtagccactc
    19561 aggtctttgt cgtaatgcca gatcatgttg ttcaggccga ttgtgacgcg gcgattgttc
    19621 tggttaatga ccttatagta atagcccgtc atccagcgca cacgccagtt atcttcgaca
    19681 tttttaccgg ttaactggtc gccactaagc gatgcccaga cgccgtttgc ttcaccttta
    19741 tcgtagctca ggcttagccc cacgccgtcg gcacgcacgc cgccccattt tttcccggta
    19801 ttgctcgggg agtctttttg cccaccaaag gccagcaaag aactggagat gggccgacgg
    19861 tgggcgttaa cggtgtaacc cagcggcccg atatcatcgc tgtaactgat gccgccgacc
    19921 acatccacca cgttgaagcc catcggcgtg gtgccgatat cccagctcca ggtgtcgttg
    19981 cgccaaccga ccgccacgct ggcaccggaa tccgactggc tgcggttgcc gctacagtcc
    20041 tgtaatgtac aggtgcccca gttgtcatcc catttaccat cggcattagt ggagaaactg
    20101 ccgacgttca tattgacgaa atcactgcga aagaacatcc gcccgtcaga atacggcgca
    20161 tccacctgca acatggtagt gtgcgctttc agatcggagt aaccaccggt gccgctcgaa
    20221 ccccagtaat catgctcaag ggtgacgtta agatcctgct ggcgatagag gtccgccgca
    20281 tcgctgcgca cgccgcgttt cagccagtca tctttctcgt cattgcgggt cagtcgggta
    20341 aaggtgtcgt tatccagcgg acgcgtcgtg gtcacaccgg atgcaaccat cgcgtctttg
    20401 taggtttcca gcgcctgcgt tggatcgccc gcctgcgctt caaacttcgc accatcacgc
    20461 agtaccatcg cactttccat cgacggcggc tgagattttg ccagcgggat caacttatta
    20521 aacgtctgct gcgccgctgc ggtatcgcca agctgcgcct gtgccagcgc cacgcgccgc
    20581 tgagtgttca gcgaggcgtt atcggtggca ggcagcttcg ccagctggct acgtgccgcc
    20641 gctttgtcac cggcagcaat atccacttcc gtcagaccaa gaatggcatc ggcgttagtt
    20701 ggctcccgcg tcaggacatt ctgatatgca gcgcgagcgg cggtgtaatc acgtcgttgt
    20761 tgcgcccagt cagccagcgt gaggtctatg cgcgtggaag gtggttgctg gcgcagcatc
    20821 gcttccgctt cagcttcttt gccgctttct cgcaggcgat tagcggtttc caacacctga
    20881 tcgctttgca gtcgattaac cagctcctga atattgctgc tccactgcgc gcgcggcagg
    20941 ctgttgatat gcgccagcgc cgctctgtcc tggtcatgac cagagagata cagcccgtaa
    21001 gcgtaaacct gctccgggtc gttcggcttc tgctgcgcca gattacgcat taacgtatcg
    21061 gcctggctgc gttgtccggc ctggctgcgt tgtccggcct gccagagatc ctgtgaaagt
    21121 cggtaagtaa tccatacact gccggggtcc agcgccagtc gttgccgctg aagtgctgcc
    21181 gcctgcgccc atttcccctg gttttccagt gcctctgcct gctgtgccag acggtcgttt
    21241 tgcaggctgc gttcgatatc atcaatgctg cgccgctggc tggcagagag cgaggcgata
    21301 tacgcttcgg ctttttctgg cgactgctgg cggtaaatat tcgccagccc gcgcacggcg
    21361 ttagtgttgc cgctgtccat acgcaaggtc tgctggtaat agcgttcggc ggcgggataa
    21421 tctttgcgcg ccatcgccac atcgcccagc ccaagcactg catagctgtc ggtgttatcg
    21481 acattacgtg cctgctggaa cagccgttct gcccggtcag gattattggc tttcagcgcg
    21541 gcatcgccct gctggatcgc cagccagtag cggtttactt tcagcagact gttccatttg
    21601 tcgttattgc tgttgtgcgg atccagcgcg agggcttttt ccagattcgc caccgcatta
    21661 gcgcgatcgc ctttctgaga atacgcctgg cccagcgccc ccagagcctc actgtctttc
    21721 gggttcgccc gcaccgcctg ttgtagttcg ggaatggctt tacccgccat gccagagtcc
    21781 accgccgcta aaccttgcgc acgagcgcgg aaagcagggt cggccagctg tttttgctgt
    21841 tctgccagtt gcgattgtgc agcagctacg ctatcgccat cgctaaagat cgagagatat
    21901 tttttcagtg cctgaacgct ggcatcgctg acaggcatgt ctttaatctg cccgtaccag
    21961 attttagagg ccccttcgcg cccggcgttc gatttcgcca tctgttccag gacggcaaaa
    22021 ccttcatcac ggcgatcgct actaaacagt aatagcgcca gattgttttg caggcccgtg
    22081 ttgcccggcg tatccgcatt aatgcgtttt agctgattaa tcgcttcgcc acgtcgggcc
    22141 ggaattttcg ccaccgtact ccagtactcg acagcaatgt caccttccgg cggcgcaccg
    22201 ttgaacagtt tgttgtaact cgccacagct tcttccgcat gaccagtcgt cgcctgcaat
    22261 cttgcctgtt gcagtgcctg acgaccatcc gacgtggaaa gcagcatcgt agtccgtgac
    22321 gatttatacg catttgaact cggcgctaac tgcgacagcc gatcgagctg tttttgtgcg
    22381 ccatcaatat cgccctgacg taacagagaa cgaaaacggg cagcaacgac gtccgggtta
    22441 ttcggatcaa taagttccag acgatacaac gactgttgca ccaggtcttc acgatgggtc
    22501 gcttcgccta accgaacttg ctccagcaac tgttgctgag cggttggtgc tgcctcgacc
    22561 atcggcatga cggccagacc gagggaaagc gtgaatatgt ttagtgtgaa tttgcgcatt
    22621 cctggcccca gtcaggtaat aactcacctt ttgtcgagaa gcggaaacgg tgttgatccc
    22681 agccttgtcc aaacagggtc agcacatagt tgtaataggc atcgctgcca ggaaagttat
    22741 cggccacgcg ctggcgctga acggcctgcg catcgcggtt ttgtaaaaag ggcagcatgg
    22801 cggcagaaaa accgaccggt cctttaccct gcgctttccc cgtagccaca tccacttttt
    22861 ccggcggata accgtttttc tcagtgaatg tcgccatcgg tttaaaccgg ttgagcatcc
    22921 gcgctttttg cggatcgctg tcaggcatca tgcctaccca catgtaaacg cggatagcgt
    22981 cgtagctgct gatcaatgtt ttttcggctt ttagctgcca gcctttgtct ttctcatagc
    23041 gcacccagtc tggcgaaaag cctttcgggg cggtttccag caataaacgt tgattggttt
    23101 cgcgcagcgt ggtccacggc gcgccaaagc gggtgaaata ctgcgccagc gtcggcggca
    23161 ggtagctggg gttaaaacgc cagctgttat cctctgcaaa tcccactttg cccggtaaca
    23221 acatggagcc cagcccaggc accgtcacca cttcctcccg cgcgatacgt tttagcaatg
    23281 cgctgccgat gtcggtataa cgctgctctt tccacaaacg ccccgcctcc agcaacgccc
    23341 aggccatcca gacatcacca tcggaggccg aattgctgtc cagcacttcc cacttactgt
    23401 tctctttctt gccccacagc caggcgggca aatgttcttt taaagaaccc tgtgcgagat
    23461 tgttctgcgt ccagtcgaga agattatcga aagctgcacg gtcgttagcc gccagggcaa
    23521 agaacatgcc gtaactttgc ccttcggagg tggtgatttt gcgcgcgtcg ctggggtcga
    23581 tgacgcgccc ttcctgactg atgtaatcct ttttaaactg ctcccaggca ggccaggtac
    23641 aggctgcctg aacactaaag gcagccagca gcagcatcgt cacgagtcca ctacgcaaca
    23701 cattcatctt caattactcg ttatccgggt taagacgacg acggctgata atacgcagca
    23761 gacgccacag tacccatgcc agcagcacga cgctgattgc cgccagcacc gccagcagaa
    23821 tcggatggtt tgccagcgca taccataagc gttcgaacca cggcagatgg ccaacgtaat
    23881 agacgtcgcc aacacgcagg ctattgatac cggactcgcg gatcaccgcg accgaaccga
    23941 acatggtggc gcgtttgccg ctatcgttca ccgcatcgtt aagcatttca taaccgcgtg
    24001 ggctatctgc cagcagcgca atcacgctac gctggtcgtt atacggcgac tggaagccaa
    24061 tcaccgctgc catcgcacct gaagaggtca gcgttgactg agtttctgcc gcgcgatcgc
    24121 tctcgtccgg tacaatgccg gggaacgggg tctggcgcat cggcgttttc acccagcttt
    24181 cggtcgcctg caccaacagg tcgatctgct tgtcgtcttt cagtttgtcc gggataccac
    24241 cgacgatcat gatgtcggca tctttcccct gaatagtgct gccatcatcg gtcaccgtca
    24301 ggttaatcgc cgggaagccc gtctgcgcac cgataaagcc aacggtattc agcaaggttt
    24361 ccatctgtgc ttcgttaggc gctttcggca tcacggtgat ggtttgtgac agatccgcca
    24421 tccggctgaa cgggaagccc gcgttagcaa aggcgcgtag atccggcatc gggatgaagt
    24481 ggtaatactt cgagaagtcg atggtggaat catcaccaat caccacatga ttctgcaccg
    24541 gctggaaggt aatgcagtta tccaccgaac cgcccggcat cgggttcata tactcaaagt
    24601 caaagcgcag ctggttggtc gcgcccagtt tcagcgccgg aatagagaca tccgttttgc
    24661 catccagcaa accttgtaat accggaatac gcagcagcag gcggttcgcc tcctgtttac
    24721 tgctcaggtt gaaggattgc aggaactggt tattcaggct gatatccatc cgtgagctgt
    24781 ctttcactgg cggcatggtg tagcgataat taatatccat atcaatgccg gtactgcgca
    24841 tcaggtagag atccggtggc aggtttagcg aaacgttaat cgctgctggc tcaagaccgc
    24901 tggattgtaa ctgttcttca taggttttca gttcaccaaa ggtaaccgga cgatcggtac
    24961 gcacccagtt cggcgcatcg tacggcttac gcggtagcag cggtttcact tcattcacta
    25021 ccacgctttc accacggaac agaatgttac cctgagcgat acctttcgct gcctgcaaca
    25081 ggtctttgtc gtcacgacca aacaccacca gcagtttgac ataaggattc tgcggatggt
    25141 tgatcatttc aatcaccggg gcttttaccg ccggatgatc gcgcaggaag tccgggcgtt
    25201 tgtcattggt ggcaaaaacg atggcattgc gatccggcaa ttggttatag agcaccggga
    25261 agttctgccc acgccagcca gaacgcgaac caaaccacga ggcgacaatg gcagatgctt
    25321 gttgcagccc gacatccggc gcacccgcaa agaccatcgg caaggtgtta gtacggttat
    25381 cacgcgggtc aaagaatggc accgggaagt gtgacaggtc attcttcaca ttcagggtct
    25441 gataggtcag atccagtcca ctgctccgcc caacatccag ccaaagcgtg gtgctggccg
    25501 ggttttcgca cacgtcctga taatgaccga caaactccag ccgcacacgg ttgaagtcgg
    25561 taataaacag tgggttaatt ggcatttgtg ccagcgtttt tttacccaac tgttctttgg
    25621 tcactggcag cacgcccatc agttcatcat tgagataaac ctttaactgc gactggacag
    25681 gcagtaatga tggcgatggc gtgtattcga ggttgagcat cgccttcgtc accacttcat
    25741 cgctgcgcat accaaactca atgctgccgt tcgggttaat gccacgtagc accatgctgc
    25801 ccggcggcgg cgcaatttgc gcaaaggtca gcttcacatc acgggaagga ccgttctgcg
    25861 ccacgactgg cgcatctgcg ccctgcacgc ccggcatcac ttgccccacc tgcggatttt
    25921 gctccgcctg ggcggctaca gctggctcag cattgatcag tggttgcgtt gctggcgtcg
    25981 cctgcgtcat gaaagagggg aacgcactca tccccagagc cactgcacaa atccagaata
    26041 tttttctttt catcgcgtta tcatcattgt tgagccaaag cctgatccga tggttgtgcc
    26101 gtttcgctcc gctccgggcg gcgcggaata aacgatacaa cccaggaaac cagagaagtc
    26161 agcacacgga atatgccctt caccgaagaa ggcgcaaatt ccgccagatg gcggtagcca
    26221 cggaagccga gcttcagaat atccagcaga ctttccagcg gcttatcttc cgggtagctg
    26281 tcctgccaga gcgcccatgt atccgcacgg gcaaacgtac actgcacaaa atcgatatgt
    26341 tgctgggtag tgagcggcat taatttcagc ccaacttcat tacccatcac acgcgccacc
    26401 tgagccggga agacgtattc ctgctgaccg cgtttaagca acagattcac tttctgccct
    26461 tccagaatct gcgcctgacc gttgatcttg atccccaaac caccgtcgga gaaatcctga
    26521 acggtacacg agaagaggtg accatcttcg cgggcaattg ctgcgggcat cgtcatctcc
    26581 acgcggtgcg atcggcgtac ctgtttgctt tctaccgata ctgcgactgc gccgccgaga
    26641 ataatcaggt tgtagaatac ccacaccata ctgacgacca cggtgagcat ctcggttggc
    26701 ggaccataga agtagcgcca gatcccaacc gctacgccca ccaggttgag cagaacaagg
    26761 aagatgtagg gccgcgagat cacccagtcg acgtactctt cttccaccag tccacctttg
    26821 gcggtgacgt taaatttgcc tttgtgcggg ttaatcagcg ccaccagcgt cggtggtgcg
    26881 atataccacg ccagcactgt ttcgtagatt tcactccaga aagagtggcg atatttgccc
    26941 tggatcttcg agttagtcag gctggcatgg atcatatgcg gtagcacgaa cagggcgatc
    27001 atcaacgctg gcgcgtagat gatgtaggca tgaaacagca ggaacgccag cggcgcagtc
    27061 aggaagatca gccgtggaat gcccgacaag aaatggaaca tggcgttgac atagcacagc
    27121 cgctgagcaa acttcagccc tttaccggtg agtgggttat cgagacggaa gatttgcacc
    27181 atcccgcgcg cccagcgaat acgctgaccg atatgcgccg agagactttc ggtcgccagc
    27241 cccgctgcct gcggaatacg catatacgct gaggtgtagc cacgacggtg cagacgcaga
    27301 gaagtgtgcg catcttcggt taccgtttcg acagcaatac cgccaatctc atccagcggc
    27361 ttacggcgaa tcaccgcaca tgaaccgcag aagaaagtgg catcccacat atcattgccg
    27421 tcctgcacca gaccatagaa cagcgtgcct tcgttcggcg ttttacgaaa acgccccagg
    27481 ttgcgttcaa acggatccgg cgagaagaag tggtgcggcg tctgcatcat cgccagctgt
    27541 ttctctttca ggaaccagcc catggtcatt tgcaggaacg atcgcgttgg tacgtggtcg
    27601 cagtcgaata tcgacacgaa ctcgcctttg gcatatttca gcgcattgtt gatgttgccc
    27661 gctttcgcat gttcatgagt ggtacgggcg atatatttca cccccacgtt ttgcgcaaac
    27721 tggcgaaact cttccctgcc gccgtcatcg aggatccaga tgttcagctt gtctttcggc
    27781 cagtcgatac ccagcgaagc gtaaatggta tttttcacca cgttgagatc ttcgttgtaa
    27841 gtcgggacaa agatatccac cgacggccac agcgacatat ctttcggcaa tggcaccggc
    27901 tgacgattca gcggccatac gacctggaag tagccgagca ccagcactat ccacgcgtac
    27961 gtttcggcga acagcagaat aagcccgcac accaggctga ccggatcgtc ccagtttaac
    28021 gtcgaggtgt aacgccacca gatataacgg caagaaacgg tcagcgacag cacaatcagc
    28081 atcagcgccg agaagcgccc cggcatccgc cgtacgatca gcgctacccc ccacagcagc
    28141 atcaggaaga taaactgcgc tagcgggtta aacggctgag taacgcagat taacgccaga
    28201 atcagcgaga aggtgacgat gataccaagg atcaaccgcc gcgccccggc actcaaatga
    28261 ccgagctctt ttttctcatc aagatgctgt gttttatggc taacgcgctc aggcagctcg
    28321 ttcatccatt gatggtaacg tccacgaata ttttgtagac ctgaaaatgc ccgcctgcgc
    28381 ggtttcggcg tttctttgcg cgatgcaccg atcagtaacc agcatgtttg aataagataa
    28441 cggaccgggt ccagcggacg cggacgcgag gcgttgatat gcggatacag gtttttatgt
    28501 tctgcgcgaa tacgctgcca gcgcgggtgc tccagcggaa taaaaatcca ggccaggatc
    28561 atccagaaac agccgagcgt cgcgctgaaa gccgacgcac cgtgacgacg ataatcgcga
    28621 taacgcccaa taagccgtgc gttgaccggc gggataagca accaccgggt caggatactc
    28681 atgatgcact cccgactggc gttttcagcc cggaataatt caacaggcac cagttcgcca
    28741 gcgtcagtat ctcttcagcc gccagcgcat cactgcgata ttcaccgact ggttgcttag
    28801 ctgccagaca ttcagccatc gcttcatcac gatgaatgag catcggcagc aatcggcgct
    28861 ggctttgcaa ccaaagctgg taaatatcgt cctgaacctg gctgccaata cggaagtcat
    28921 taatcaaaat atgtgcacca tccggcagcg cttgctgatg cagtcggata tggcagttgg
    28981 catccacatt gacgattgcc agcgagtgat cgcacaaact cagcagctgg tgggttatct
    29041 gcgaggcatc acgcggtaag tcaattaaaa tccactggta acgcccgctg gcttttagtt
    29101 gctgtaagcc ggtgcaaata tcgctcagcc gggtttgcca gtgctgtgga ttttcttgtt
    29161 cttcaatcga taactgacca aaaggcagca aatcgagctg cgaggtatag cgcaacccgg
    29221 cgtcacgcca gtcctggcca tccagcatag ctctggccca accctgacgg tgggtaaaat
    29281 caacgttaaa tgacaggcgc aacaagttgt ccgggcaggc atcgaccacc aggacatttt
    29341 ctcccaacat ttgtaatgac caggctaatg cggcggtg
//
LOCUS       DAZGTJ010000066        28712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000066 DAZGTJ010000000
VERSION     DAZGTJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.066"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1297)
                     /gene="yjgL"
                     /locus_tag="ACHK8E_003385"
                     /pseudo
     CDS             complement(1..1297)
                     /gene="yjgL"
                     /locus_tag="ACHK8E_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
     gene            complement(1420..1872)
                     /gene="tabA"
                     /locus_tag="ACHK8E_003386"
     CDS             complement(1420..1872)
                     /gene="tabA"
                     /locus_tag="ACHK8E_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP0345607.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(2017..2610)
                     /gene="bdcR"
                     /locus_tag="ACHK8E_003387"
     CDS             complement(2017..2610)
                     /gene="bdcR"
                     /locus_tag="ACHK8E_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0345608.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            2681..3394
                     /gene="bdcA"
                     /locus_tag="ACHK8E_003388"
     CDS             2681..3394
                     /gene="bdcA"
                     /locus_tag="ACHK8E_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0345609.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3526..3921
                     /gene="yjgH"
                     /locus_tag="ACHK8E_003389"
     CDS             3526..3921
                     /gene="yjgH"
                     /locus_tag="ACHK8E_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0345610.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            4202..4336
                     /gene="pyrL"
                     /locus_tag="ACHK8E_003390"
     CDS             4202..4336
                     /gene="pyrL"
                     /locus_tag="ACHK8E_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP0345611.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4340..5275
                     /gene="pyrB"
                     /locus_tag="ACHK8E_003391"
     CDS             4340..5275
                     /gene="pyrB"
                     /locus_tag="ACHK8E_003391"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP0345612.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5288..5749
                     /gene="pyrI"
                     /locus_tag="ACHK8E_003392"
     CDS             5288..5749
                     /gene="pyrI"
                     /locus_tag="ACHK8E_003392"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP0345613.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5822..6208
                     /gene="ridA"
                     /locus_tag="ACHK8E_003393"
     CDS             5822..6208
                     /gene="ridA"
                     /locus_tag="ACHK8E_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP0345614.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6414..9110)
                     /gene="mgtA"
                     /locus_tag="ACHK8E_003394"
     CDS             complement(6414..9110)
                     /gene="mgtA"
                     /locus_tag="ACHK8E_003394"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP0345615.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(9251..9304)
                     /gene="mgtL"
                     /locus_tag="ACHK8E_003395"
     CDS             complement(9251..9304)
                     /gene="mgtL"
                     /locus_tag="ACHK8E_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP0345616.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9489..10436
                     /gene="treR"
                     /locus_tag="ACHK8E_003396"
     CDS             9489..10436
                     /gene="treR"
                     /locus_tag="ACHK8E_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP0345617.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAYWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10555..11976
                     /gene="treB"
                     /locus_tag="ACHK8E_003397"
     CDS             10555..11976
                     /gene="treB"
                     /locus_tag="ACHK8E_003397"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP0345618.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12026..13681
                     /gene="treC"
                     /locus_tag="ACHK8E_003398"
     CDS             12026..13681
                     /gene="treC"
                     /locus_tag="ACHK8E_003398"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP0345619.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            14066..16204
                     /gene="nrdD"
                     /locus_tag="ACHK8E_003399"
     CDS             14066..16204
                     /gene="nrdD"
                     /locus_tag="ACHK8E_003399"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP0345620.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16362..16826
                     /gene="nrdG"
                     /locus_tag="ACHK8E_003400"
     CDS             16362..16826
                     /gene="nrdG"
                     /locus_tag="ACHK8E_003400"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP0345621.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16871..17257)
                     /gene="cybC"
                     /locus_tag="ACHK8E_003401"
     CDS             complement(16871..17257)
                     /gene="cybC"
                     /locus_tag="ACHK8E_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0345622.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17440..18792)
                     /gene="pmbA"
                     /locus_tag="ACHK8E_003402"
     CDS             complement(17440..18792)
                     /gene="pmbA"
                     /locus_tag="ACHK8E_003402"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP0345623.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18886..19437
                     /gene="yjgA"
                     /locus_tag="ACHK8E_003403"
     CDS             18886..19437
                     /gene="yjgA"
                     /locus_tag="ACHK8E_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP0345624.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19595..20968)
                     /gene="mpl"
                     /locus_tag="ACHK8E_003404"
     CDS             complement(19595..20968)
                     /gene="mpl"
                     /locus_tag="ACHK8E_003404"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP0345625.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21144..22142
                     /gene="fbp"
                     /locus_tag="ACHK8E_003405"
     CDS             21144..22142
                     /gene="fbp"
                     /locus_tag="ACHK8E_003405"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP0345626.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22175..23170)
                     /gene="yjfF"
                     /locus_tag="ACHK8E_003406"
     CDS             complement(22175..23170)
                     /gene="yjfF"
                     /locus_tag="ACHK8E_003406"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP0345627.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23157..24179)
                     /gene="ytfT"
                     /locus_tag="ACHK8E_003407"
     CDS             complement(23157..24179)
                     /gene="ytfT"
                     /locus_tag="ACHK8E_003407"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP0345628.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(24193..25695)
                     /gene="ytfR"
                     /locus_tag="ACHK8E_003408"
     CDS             complement(24193..25695)
                     /gene="ytfR"
                     /locus_tag="ACHK8E_003408"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP0345629.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25835..26791)
                     /gene="ytfQ"
                     /locus_tag="ACHK8E_003409"
     CDS             complement(25835..26791)
                     /gene="ytfQ"
                     /locus_tag="ACHK8E_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP0345630.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27101..27631
                     /gene="ppa"
                     /locus_tag="ACHK8E_003410"
     CDS             27101..27631
                     /gene="ppa"
                     /locus_tag="ACHK8E_003410"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP0345631.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28004..28510)
                     /gene="mug"
                     /locus_tag="ACHK8E_003411"
     CDS             complement(28004..28510)
                     /gene="mug"
                     /locus_tag="ACHK8E_003411"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP0345632.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28635..28710
                     /locus_tag="ACHK8E_003412"
     tRNA            28635..28710
                     /locus_tag="ACHK8E_003412"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28668..28670,aa:Ile,seq:cat)
BASE COUNT         7137 a         7588 c         7082 g         6905 t
ORIGIN      
        1 aatatgattt ttctcgattg cttaagttaa aatcattgac atatacaacc aactttttcc
       61 aaatcaactc ctgtaattca gggctatttt tatagggttc tttatcgaga ttgaaaaata
      121 atgtctcagc tactttagac tgatcgcaga gaaaattaat atgatttatt acggagcatg
      181 catgctctat ctttgcagaa tcatcaaaat attgcatgct tttattaaaa caccactcca
      241 gtatttttac atcattatta tatggcaatt ctaaaaaagt caccaacatc gaatggatga
      301 ccggtgtaag gttaaccttt gcatcatcta aggaatcaat aagaatatga gacaggttaa
      361 tcatgatgtc attaatttca ttgttacctt ctttgtcatt atcaattccg tgataattta
      421 attcgtagag cttatctatt tcttcaagca taccattttc gcccagtaag tatgaaatcg
      481 attcatcaca tacatgaggc attaatagca gatgttcgtt ccttattgct aataaggctt
      541 tattcaatgc ttattctaat aaatccgtaa tcgtagaatc aactgaacct ttacaactca
      601 cctgcgagtt atttaagtcc tgcgagttat ttaactcctg agaatcattt aaatcctgcg
      661 aattatttaa ctcctgcgaa ttatttaact cctgtatatt atttaacttc tgtaaattat
      721 ttaacttctg atgattattt aatagacgcg ctatttcctt gttagcttcg cgttggtaac
      781 acgccagaac cttgttagta atcaggtgta atgaactatc atcttttctt tcaaaagact
      841 tcccttcata acgattagtc gaaccatcag attgttgaaa tataatagcc gtatgtcctt
      901 gacgagtccc catagtagtg atagtggaaa gtgtaatttc tatttccacc ttagataaat
      961 ccgctcgttc cccttcagga ttattcatgt aaaacttcat tttttcattg ccatcagtat
     1021 ctttagaata agatgaggac tcaatatgag ccatcatatt tctacccgct tcgttggagc
     1081 gtttgctaaa atccacataa cccagggtaa atatctttgc caaaaactct aaaaatgctc
     1141 caggagaaag aatattcctt ctgctatcct gccccccggc aatacgggca aaatcattca
     1201 ttccaacacg ccaggtattt aaaacttcac ttttttgttt aaaattgtcg gctaatgtaa
     1261 tgtgttgaga ataattcaaa tctgatattt tgctcatgac gaactccttg agttaataag
     1321 aacgtctaag cattacaaat tttactcaca aaaaaatatc aaaaaacgct cttttcacaa
     1381 actcgccgta aacctggagt tatttacggc gaaaagtcat cacgccatca gcatcttcac
     1441 tactgctttg cgaacccggg ctggcgcgcc cactgcgcac agcggtttat gcacttcccc
     1501 cggataaaac acaacaaaat caccttcatt aaggataact gttttctcat caacgccttc
     1561 cggcaaaaat gcgatgtctt tatcagctaa ccagtcggta tccggcgtac ctgcaggttg
     1621 tgtgctgaag gtcatgcctt cctgaccttt taacacaatc tgaatgtcga gatagcgggc
     1681 atggtactcc gcacggcgag cttcgtacgg ctcggtcata tcttccgaga taagataaaa
     1741 cagacgattg ccttcgatat cgtgcttgcc cttgggcgtt tctgccgtaa cgtttgcttt
     1801 gatatgctca atcgcctggc gtaactcttg gggtagccac ggctgaagat tatgaatatt
     1861 tccgatgatc atcttttcca ctccttaatt aaaacaacgt ttcatttcta tcgttatacg
     1921 aaaagatgat caattccgct agccttaacg cgtgaattat tgtcttatca caacttatcg
     1981 gcaggcgcgt tcccgccttt cgggtcaagg cccgcatcac tcctcgagaa tagtttttat
     2041 cgcttccccc gccagggcag ctgttgcaca gagttgttcg atggagtgcc cctcccgtgc
     2101 cttcgcagaa agccctgaca tcacggtact cataaaatca gtcacacatt gtgcgctttg
     2161 tggatgccgc ctggcgatat agtcataaat ggtcgtttct gcggcgtgat aatattgaat
     2221 ggcaatatca cgcgcttgtg gatcatgact atgaatacct tcaagaacca tacagccagc
     2281 gcagccgccg ttttggctat atctgcgcgc cgcttctttt aatacctcaa ccaggcactc
     2341 gcctaccgga cgatcatcac gaagaatatc ggcaagcgga atagcttccg taccgacata
     2401 ttcattgagt acacgactaa acaacccagc cttactgcca aaagccgcgt agaggctcgg
     2461 ggggttaata ccaagataat cagtaacttc agagacactt actgcatcga aacctttttg
     2521 gtgaaaaagc acttttgccg cagagactgc ctgctcagga gcgaaacgtc tggggcgacc
     2581 tggaacgcga gattgttttt tagtgaccat tacaaaactt gttgacagaa agttaaaaca
     2641 gttttgtaat gcatgttaca taataaatca aggagtcctt atgggcgctt ttaccggtaa
     2701 gacagttctc atcctcggtg gtagccgtgg aatcggtgcc gctatcgtac gtcgtttcgt
     2761 caccgatgga gccaatgtac gattcaccta tgcggggtcg aaagatgccg ctgaacgtct
     2821 ggcacaagag actggagcga cagcagtatt cacagatagt gctgacagag acgctgttat
     2881 tgatgtcgtt cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag gtattggcgt
     2941 ctttggcgat gccctggaat taaatgccga cgatattgat cgccttttca aaatcaatat
     3001 tcatgctcct tatcatgcct ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat
     3061 cttaatcatc ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc
     3121 cgccagtaaa tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg
     3181 agggatcacc atcaacgtcg tccagccagg accaattgat accgacgcta atcccgccaa
     3241 cgggccaatg cgcgatatgt tgcatagttt tatggccatc aaaagacatg ggcaaccgga
     3301 agaggtcgct ggtatggtcg catggttagc agggccagaa gccagttttg ttaccggcgc
     3361 gatgcatacc attgatggcg cgtttggcgc ataaccgact acgctcaatt aagcccagcc
     3421 attatccatg atgtctgggt tttgtttact aacgtcgtcc ccctaaaagc ggctcccggt
     3481 aaatataaat cttctgttca tgtcgtaaat cttgaggata accaaatggt agaaagaacc
     3541 gctgttttcc ctgctggccg acattcactg tatgctgagc atcgttattc tgcggctatt
     3601 cgttccggcg atttgctgtt tgtttccggg caagtaggaa gtcgagagga cggaacacca
     3661 gaacccgatt ttcagcaaca agtcagactg gcatttgata atttgcatgc gaccctggct
     3721 gctgcgggat gcacttttga cgatatcatt gatgttacga gctttcatac cgatccagaa
     3781 aaacaatttg aagacatcat gacggtcaaa aatgaaatat ttagcgcccc accttatcca
     3841 aactggacgg cggtgggtgt tacctggctg gcaggctttg attttgaaat taaagtgata
     3901 gcgcgcatcc ctgagctata agcaatagtg ttagccgttc gttttcacac tccgccctat
     3961 aagtcggatg aatggaataa aatgcatatc tgattgcgtg aaagtgaaaa agaaaaaagc
     4021 aggaaatgtc tgcaattatt gataccgaac gacagttctc ccccagaatc acatcaaata
     4081 aaaatgcatg tattttgact tttaattcaa ataaaccgtt tgcgctgaca aaatattgca
     4141 tcaaatgctt gcaccgcttc tgacgatgag tataatgccg gacaatttgc cgggaggatg
     4201 tatggttcag tgtgttcgac attttgtctt accgcgtctg aaaaaagacg ctggcctgcc
     4261 gtttttcttc ccgttgatca cccattccca gcccctcaat cgaggggctt ttttttgccc
     4321 aggcgtcagg agataaaaga tggctaatcc gctatatcag aaacatatca tttccataaa
     4381 cgaccttagt cgcgatgacc ttaatctggt gctggcgaca gcggcgaaac tgaaagcaaa
     4441 cccgcaacca gagctgttga agcacaaagt cattgccagc tgtttcttcg aagcctctac
     4501 ccgtacccgc ctctctttcg aaacttccat gcaccgcctg ggtgccagcg tggtgggctt
     4561 ctccgacagc gccaatacat cactgggtaa aaaaggcgaa acgctggccg ataccatttc
     4621 ggttatcagc acttacgtcg atgcgatagt gatgcgtcat ccgcaggaag gtgcggcgcg
     4681 cctggccacc gaattttctg gcaatgtacc ggtactgaat gccggtgatg gctccaacca
     4741 acatccgacg caaaccttgc tggacttatt caccattcag gaaacccagg ggcgtctgga
     4801 caatctccac gtcgcaatgg ttggtgacct gaaatatggc cgcaccgttc actccctgac
     4861 ccaggcgcta gcgaagttcg acggcaaccg tttttacttt atcgcgccgg atgcgctggc
     4921 aatgccgcaa tacatccttg atatgctcga tgaaaaaggg atcgcctgga gtctgcacag
     4981 ctctattgaa gaagtgatgg cggaagtaga catcctgtac atgacccgcg tgcaaaaaga
     5041 gcgtctggac ccgtccgagt acgccaacgt gaaagcgcag tttgttcttc gcgccagcga
     5101 tctccacaac gccaaagcca atatgaaagt gctgcatccg ctgccgcgtg ttgatgagat
     5161 tgcgacggat gtggataaaa cgcctcacgc ctggtacttc cagcaagcag gcaacgggat
     5221 tttcgctcgc caggcgttac tggcactggt tctgaatcgc gatctggtac tgtaagggga
     5281 aatagagatg acacacgata ataaattgca ggttgaagct attaaacgcg gcacggtaat
     5341 tgaccatatc cccgcccaga tcggttttaa gctgttgagt ctgttcaagc tgaccgaaac
     5401 ggatcagcgg atcaccattg gtctgaacct gccttctggc gagatgggcc gcaaagatct
     5461 gatcaaaatc gaaaatacct ttttgagtga agatcaggta gatcaactgg cattgtatgc
     5521 accgcaagcc acggttaacc gtatcgacaa ctatgaagtg gttggcaaat cgcgccccag
     5581 tctgccggag cgcatcgaca atgtgctggt ctgcccgaac agcaactgta tcagccatgc
     5641 cgaaccagtt tcttccagct ttgccgtgcg aaaacgcgcc aatgacatcg cgctcaaatg
     5701 caaatactgt gaaaaagagt tttcccataa tgtggtgctg gccaattaat tgcggttggt
     5761 aataaaagtc tggctcccta taatgagcca gactttttac cgctgtaata aaggagaaat
     5821 catgagcaaa actatcgcga cggaaaatgc accggcagct atcggtcctt acgtacaggg
     5881 cgttgatctg ggcaatatga tcatcacctc cggtcagatc ccggtaaatc cgaaaacggg
     5941 cgaagtaccg gcagacgtcg ctgcacaggc acgtcagtcg ctggataacg taaaagcgat
     6001 cgtcgaagcc gctggtctga aagtgggcga catcgttaaa accaccgtgt tcgtgaaaga
     6061 tctgaacgac ttcgcaaccg taaacgccac ttacgaagcg ttcttcaccg agcacaatgc
     6121 caccttcccg gcacgttctt gcgttgaagt tgcccgtctg ccgaaagacg tgaagattga
     6181 gatcgaagca atcgctgttc gtcgctaatc ttgatggaaa tccgggctat catgcccgga
     6241 ttaagtctga tgacaaaagc aaaatcgccc gatgcgctac gcttatcagg cctacgtgat
     6301 tcctgcaatt tattgaattt gtaggccgga ttgggcgttt acgccgcatc cggcatgaac
     6361 aaagctcact ttgtctgcaa tctgaatccg ggccaccatg cccagtttat tctttactgc
     6421 cagccgtaac gacggctata gacccctttc accaactggg ttaacgtcat ataccctgcc
     6481 agaatcgcaa ccagccacgg gaaatagctt aacggcagcg cctgcagctg cagataactg
     6541 gccagcggtg aaaacggcaa tgcgatcccg acaatcatca cgatcacggt catgatcatt
     6601 aacggccacg atgcacagct ctgaataaac ggtacacgac gggtacggat catatgcaca
     6661 atcagcgttt gcgacagtaa gcccaccaca aaccagcccg actggaacag cgtttgcgtt
     6721 tccggcgtgt tggcatggaa tacccaccac atcaggcaaa acgtcagaat atcgaagatc
     6781 gagctgatcg gtccgaagaa gatcataaag cgccccagat ccgccggatt ccaacgctgc
     6841 ggcttttgaa tttgctcgtc gtcgacgtta tcaaacggga tcgccacctg tgacacatcg
     6901 tacagcaagt tctgaataag caagtgtaac ggcaacatcg gcaggaaggg caagaaagca
     6961 ctcgccacca gcacgctgaa cacattaccg aagttagaac tcgccgtcat tttgatgtat
     7021 ttcagcatgt tggcgaaagt gcgacgtccc tcaataaccc cctcttccag caccatcagg
     7081 cttttttcca gcaggatgat atcagccgct tcacgggcaa tatctaccgc gccgtccaca
     7141 gaaatgccga tatccgcagc gcgtaaggcg ggcgcatcat taataccatc gcccataaag
     7201 ccaaccacat gcccttcgcg cttcagtaag gtcacaatgc gttctttatg catcggcgtc
     7261 aggcgggcaa acagcgtggt gcgctgtgcg agatttgcca gttcatcgtc agatagcgtt
     7321 tcaatatcac taccaatgac cacctctccc gcatccagcc ccacttcatg gcacactttc
     7381 gctgcaacta actcactgtc gccagtaagg atttttacgg taattccact cgcttttaat
     7441 gccttcagtg ccggagcggt tgtctctttt ggcggatcaa ggaaagcaat atatccttcg
     7501 aggatcaggt cggattcatc cgcccgctgg taatctcctt cacgcgctgg caggtatttc
     7561 gtcgccaccg caaccacgcg cagcccctga cgattcagcg tatcagtaac ccgcttaatc
     7621 ttacgcagca tgatgtcatc gagcggcaca atctcgccat tgtgacgcac ctgcgaacac
     7681 acattgagga tttcctgcaa tgcacctttg caaaccagct gatggtgttc ggtattttct
     7741 gccactacca ccgacatccg acgacgctcg aaatcaaacg gaatctcatc aattttttgc
     7801 caacggctgg ccagcgaacg cgctgactct tcatccgtac cttcgagcac cgcggtatca
     7861 agcaggtttt taagtccggt ctggtaatga ctgttcaacc acgcgctatg cagcacgcgt
     7921 tcgctggttt taccggagat atcggtatga ttctccagca caattttgtc ctgcgtcagg
     7981 gtgccggttt tatcagtgca cagaatatcc attgcgccaa agttctgaat ggcatccaga
     8041 tgtttgacga tcactttctg tttcgacagc tttactgcgc cgcgcgccag cgtcgaggta
     8101 acaatcatcg gcaacatttc tggcgttaag cctaccgcga ccgaaagcgc aaacagtgcc
     8161 gcttcccacc agtcgccttt ggtgtaaccg ttgataagca acaccaccgg agccatcacc
     8221 agcataaagc gaatcagcag catactgacg cggctaatcc cttgctgaaa ggcattcggc
     8281 tcgctttcct gctcactaac acgccccgcc agttgaccaa accaggtatt ggcccctgtg
     8341 gcaatcacca ttgcttgtgc cgtaccgctc accacggtgg tgcccataaa acacagggtg
     8401 tcgcactcca gcggattgct gtgctccggc tggcgagtag tagcggcttt ttccacgggc
     8461 agagactcac cggttaacga cgcctgagcg acgaacagat cccgcgcctg caagatacgt
     8521 aaatctgccg ggatcatatc ccctgccgcc agttttataa tatcgccggg caccagttgg
     8581 tcgatcggga tctccagcca gccattttcg cctttgtcgt taattacgcg cagcaccgtc
     8641 gcagtattgc tgaccatcgc tttcagggca tctgccgctt tagtggaacg tgcttcctga
     8701 ataaagttca gcaacgtaga aatagcgacc attagcgcga taacgcccgc ggcaaataaa
     8761 tcttccgtgg cgtaagaaat agcgccgaga atggtgagta aaatattaaa ggggttgcga
     8821 tagcagaccc ataaatgaac ccaccacggc gacggttgtt gtgcgggtaa tttattttca
     8881 ccatgttgtt cgcgggcaga ttccacttcc gcctgattta atccttccgg atgggtgtcg
     8941 aacgttttcc acaattcttc ttcgggcatc accgccattt tcaggcaatg cgcacttaag
     9001 gacggcggga ccgccgtatt cactgtctgc tgcgcgccag gcaatggatc gcgatgaacc
     9061 agacgggaag gtaaatggcg aatgaaccgg gtaaaaattt ctttaaacat aaggagtccc
     9121 tccgcactgt ctgaaacagt acagaaatgt cttcaggcgt ggcgaagcct tacctgaagg
     9181 taataaagct gagcagggac gttaaaacgt cacggcctta cgcatccggt aacgccgtga
     9241 aaagacgggc ttaccggaaa agttttaatc tccgtcgagg gagaggcgtg ggatcaggtt
     9301 ccatataacc tccggtaagt gaaaaaagcg ccgcaaataa taaggagaat ccaataatat
     9361 tcgtagcatt tatggcgata taatacctgc tggcaactaa accaacgata aaccagactt
     9421 tacccattgc tgaatgcaca ggtaaagtta ggctcaaata attaaacaac acgttatggg
     9481 acaacaggat gcaaaatcgg ctgaccatca aagacatcgc gcgcttaagc ggcgtgggga
     9541 aatctacggt ttcccgggtg ctgaataacg aaagcggcgt gagccagcgc acgcgcgagc
     9601 gtgttgaagc agtgatgaat cagcatggat tttccccttc ccgctctgcg cgcgctatgc
     9661 gtgggcaaag cgataaagtg gtcgccatca ttgttacccg tctggattcg ttgtcagaaa
     9721 atctcgccgt tcaaaccatg ctgccagcgt tctatgaaca aggttacgat ccaatcatga
     9781 tggaaagtca gttttcccca caattagttg ccgaacattt gggggtgctg aaacggcgta
     9841 atatcgacgg cgtagtgctg ttcggtttta ccggcataac agaagaaatg ttagcctact
     9901 ggcagtcatc gctggttctg ctggcgcgtg acgcaaaagg ctttgcttcg gtctgttatg
     9961 acgacgaagg ggcaatcaag atcctgatgc aacggctgta tgaccaggga catcgtaata
    10021 tcagttatct cggcgtgccg cacagtgacg tgacgaccgg taagcgacgt cacgaagcct
    10081 acctggcgtt ctgcaaagcg cataaattgc atcccgttgc cgctctgcca gggcttgcta
    10141 tgaagcaagg ctatgagaac gtagcaaaag tgattacgcc tgaaactacc gcgttactgt
    10201 gcgcaaccga cacgctggca cttggcgcaa gtaaatacct gcaagagcaa cgcatcgaca
    10261 ccttgcaact ggcgagcgtc ggtaatacgc cgttaatgaa attcctccat ccggagatcg
    10321 tcaccgtaga tcccggttac gccgaagctg gacgccaggc ggcctgccag ttgatcgcgc
    10381 aggtaaccgg gcgcagcgaa ccgcaacaaa tcatcattcc cgccaccctg tcctgatcgt
    10441 ttcctgaacg ataaattgtg atcttcgctg cgtttcggga acgttcccgt ttttaaattt
    10501 ttcagcgcaa tatattctgc agccaaccaa aaatgtcatc tgccatgggg ctttatgatg
    10561 agcaaaataa accaaacgga tatcgatcgg ttgattgaac tggtcggcgg gcgcggcaat
    10621 attgcgacgg tgagccactg tattactcgt ctgcgctttg tcctcaacca accggccaat
    10681 gccagaccga aagaaattga gcaactcccc atggtgaaag gctgtttcac caatgccggg
    10741 caatttcagg tggtgattgg caccaacgtg ggtgattact atcaagcact gatagcgtca
    10801 accggacagg cgcaggttga taaagagcag gtaaaaaaag ccgcccggca gaatatgaaa
    10861 tggcatgagc agttgatctc tcatttcgcg gagattttct tcccgttgct gcccgcgttg
    10921 attagcggcg gtttgatcct cggttttcgc aatgtgatcg gcgatttgcc catgagcaac
    10981 ggtcaaacgc tggcgcaaat gtacccttcc ctgcaaacga tctacgattt tctgtggttg
    11041 atcggtgaag cgatcttctt ctacctgccg gtcgggattt gctggtcagc ggtgaaaaaa
    11101 atgggcggca cgccgatcct tggtatcgtg cttggcgtaa cgctggtttc tccacagctg
    11161 atgaacgctt atctgctcgg gcagcagctg ccggaagtgt gggactttgg catgttcagc
    11221 atcgccaaag tgggctatca ggcgcaggtg atcccggcac tgttagccgg gctggcgctg
    11281 ggcgttattg aaactcgtct taagcgcatc gtgccggatt acctctatct ggtggtggtg
    11341 cccgtttgtt cgctgatcct cgcggtgttc ctcgcccatg cgctgattgg tccgtttggt
    11401 cgcatgattg gcgatggcgt tgcctttgcg gtacgtcacc tgatgaccgg cagctttgct
    11461 ccgattggcg cggcattgtt tggcttcctg tacgccccgc tggtgatcac cggcgtacat
    11521 cagaccacgc ttgctattga tttgcagatg attcaaagca tgggtggtac gccagtgtgg
    11581 ccgctgattg cgctgtcgaa tatcgctcag ggttccgccg tgataggcat tatcatttcc
    11641 agccgcaagc acaatgaacg cgagatctcc gtgcctgccg ctatctccgc ctggcttgga
    11701 gtcactgagc ctgctatgta cggcatcaac ctgaaatatc gcttcccgat gctgtgcgcg
    11761 atgattggtt ctggtctggc aggattgcta tgcggcctga acggcgttat ggcgaatggc
    11821 atcggcgtag gcggcctgcc gggaattctc tcgattcaac cgagctactg gcaggtgttt
    11881 gcgctggcaa tggctatcgc catcatcatc ccgattgtac tcacctcgtt tatctatcag
    11941 cggaaatacc gcctgggcac gctggacatt gtttaatttt cttcggggcg caattgcgcc
    12001 ccctcgcatt tgcaggaata acgtaatgac taatcttccc cactggtggc aaaacggcgt
    12061 tatctaccag atttatccaa agagttttca ggacaccacg ggtagcggca ccggcgattt
    12121 acgtggcgtt atccagcgcc tggactatct gcataaactg ggcgttgatg ccatctggct
    12181 aacccccttt tatgtctctc cccaggttga taacggttac gacgtagcga actatacggc
    12241 tattgatccc acctacggca cgctggacga ttttgacgaa ctggtgacac aggcaaaatc
    12301 gcgcgggatt cgtatcattc tcgatatggt gtttaaccat acctctaccc aacatgcctg
    12361 gtttcgcgag gcgctgaaca aagcaagccc ttaccgccag ttttatatct ggcgcgatgg
    12421 agaatcagaa acgccaccga acaactggcg ttcaaaattt ggcggtagtg cgtggcgctg
    12481 gcatgcggaa agcgaacagt actatttgca tctctttgca ccagaacagg cggatctcaa
    12541 ctgggagaat ccagcggtac gcgcagagct gaaaaaagtc tgtgagttct gggccgatcg
    12601 tggggtcgac gggttgcgcc tggatgtggt gaatctgatc tccaaagacc cgcgtttccc
    12661 tgatgacctg gatggcgacg ggcgtcgctt ctacaccgac gggccacgag cacacgagtt
    12721 tttgcacgag atgaaccgcg atgtgtttac gccacgcagg ttaatgaccg taggtgaaat
    12781 gtcctccacc agccttgagc attgccagcg atatgcgaca ctgacaggca gtgaattgtc
    12841 gatgaccttt aattttcatc acctgaaagt cgattatccc agtggtgaaa aatggacgct
    12901 ggctaaacct gactttgtgg cgttgaaaac attgttccgc cactggcaac aaggaatgca
    12961 caacgtggca tggaatgcct tgttctggtg taaccacgat cagccgcgca ttgtttctcg
    13021 ctttggtgat gaaggtgaat accgcgtgcc tgcggcaaaa atgctggcga tggtgctgca
    13081 tggcatgcag ggaacgccgt atatctacca gggcgaagag attggcatga ccaacccgca
    13141 tttcacgcgc attactgact atcgcgacgt ggagagcctc aatatgtttg ccgagctgcg
    13201 caacgatggt cgtgatgccg acgagttatt ggcaatcctc gccagtaaat cccgtgacaa
    13261 cagtcgcaca cccatgcaat ggagcaacgg cgataatgcc gggtttacgg ctggcgaacc
    13321 gtggattggc ctgggcgata actatcagga aatcaacgta gaagcagcgc tggccgatga
    13381 gtcctcggtg ttttacacct accaaaagtt aatcgcactg cgtaagcaag aagccgtcct
    13441 gacatggggc gattaccagg atctgctgcc aaacagccct gtattgtggt gctatcgccg
    13501 tgagtggaag gggcaaacct tactggtcat tgccaacctt agccgtgaga cccaaccctg
    13561 gcagccaggg aaaatgctcg gcaactggca gcttgtgatg cataactacg aagaagcctc
    13621 accacaaccc tgtgccatga ctttacggcc ttttgaggct gtctggtggt tacagaagta
    13681 agtatccgct aagccccggt aatactggtc attccggggt tttgcctttc agcatgttga
    13741 ttttataatc aaaaaattca ttaaaaaatt tacataacgc tgtagcgccc gtcatccgta
    13801 cgctctgctt tttactttga gctacatcaa aaaaagttca aacatccttg atgcaaagca
    13861 ctatatatag actttaaaat gcgtcccgac ccaatatgtt gtattaatcg actataattg
    13921 ctactacaac tccccacgaa aaaggtgcgg cgttgtggat aagcggatgg cgattgcgga
    13981 aagcgccgga aaacgaaacg aaaaaaccgg aaaacacctt tcccaatttc tgtggataac
    14041 ctgttcttaa aaatatggag cgatcatgac accgcatgtg atgaaacgag acggctgcaa
    14101 agtgccgttt aaatcagagc gcatcaaaga agcgattctg cgtgcagcta aagcagcgga
    14161 agtcgatgat gcagattatt gcgccactgt tgccgcggtt gtcagcgagc agatgcaggg
    14221 ccgcagccag gtggatatca acgagatcca gaccgccgtt gaaaaccagc ttatgtcggg
    14281 tccatacaaa caactggctc gtgcttacat cgagtaccgt cacgatcgcg acattgaacg
    14341 tgaaaaacgc ggtcgcctga atcaggagat ccgtggtctg gttgagcaga ccaacgcctc
    14401 gttgctcaac gaaaacgcca acaaagacag caaggtgatt ccaacccagc gcgacctgct
    14461 ggccgggatt gtggctaaac actatgcacg ccagcacctg ctgccgcgtg acgtggtgca
    14521 ggcacatgaa cgtggtgata ttcactatca cgatctcgat tactcaccgt tcttcccgat
    14581 gttcaactgc atgttgatcg acctgaaagg catgctgacc cagggcttta aaatggggaa
    14641 cgccgagatt gaaccgccga agtcgatctc tacggcaacc gcggtaactg cgcagattat
    14701 tgcccaggtc gccagccata tttatggcgg caccaccatt aaccgtatcg atgaagtgct
    14761 ggcaccgttt gtcaccgcca gctacaacaa acatcgcaag accgcagaag agtggaacat
    14821 cccggacgcc gaaggctacg ctaactctcg taccatcaaa gagtgctacg atgccttcca
    14881 gtcgctggag tacgaagtaa acaccctgca caccgccaac ggtcaaacgc cgtttgtaac
    14941 ctttggtttt ggtctgggca ccagctggga gtcgcgcctg attcaggaat ctatcctgcg
    15001 taaccgtatc gccggcctcg gtaaaaaccg taaaacggcg gtgttcccga aactggtgtt
    15061 tgcgattcgc gatggcctga accataaaaa aggcgatccg aactacgaca tcaaacagct
    15121 ggcgctggag tgcgcaagca agcgcatgta tccggatatc ctgaactacg atcaggtagt
    15181 gaaagtcacc ggttcgttta aaaccccgat gggctgccgc agcttcctcg gcgtgtggga
    15241 aaatgaaaac ggcgagcaga tccacgatgg tcgtaacaac ctcggcgtga tcagcctgaa
    15301 cctgccgcgt attgctctgg aggcaaaagg cgatgaagcc accttctgga agctgctgga
    15361 tgaacgtctg gtgctggcac gtaaggcgtt gatgacccgt atcgctcgtc tcgaaggcgt
    15421 gaaagcgcgc gtggccccga tcctctatat ggaaggtgct tgtggcgtgc gtctgaatgc
    15481 tgacgacgac gtttctgaaa tcttcaaaaa cggtcgtgcg tctatttcgc tgggttacat
    15541 cggcatccac gaaaccatta acgcgctgtt cggcggcgag catgtttacg acaacgagca
    15601 gctgcgcgcg aaaggtattg cgattgttga gcgtctgcgt caggcggtgg atcagtggaa
    15661 agaagaaacg ggttatggtt tcagtctcta cagcacgcca agtgaaaacc tgtgcgaccg
    15721 cttctgccgt ctcgataccg ctgagtttgg cgtggtgccg ggcgtgaccg ataaaggtta
    15781 ctacaccaac agtttccacc ttgatgtgga gaaaaaggtg aacccgtatg acaagatcga
    15841 ctttgaagcg ccttacccgc cgctggcgaa cggtggtttc atttgctacg gcgagtatcc
    15901 aaacattcag cacaacctga aggcgctgga agatgtctgg gattacagct atcagcatgt
    15961 accgtattac ggcaccaata cgccgatcga tgagtgctac gagtgtggct ttaccggtga
    16021 gttcgagtgc accagcaaag gcttcacttg tccgaaatgt ggtaaccatg acgcctcccg
    16081 tgtgtcggtg actcgccgcg tgtgcggata tttaggtagc ccggatgcac gtccatttaa
    16141 cgccggtaag caggaagaag ttaagcgccg cgttaaacat ttggggaatg ggcagatagg
    16201 ttaatcttca ctctgcgtta agcaaatgcc ggatgcggcg taaacgcctt atccggccta
    16261 catttggcaa cggctgtagg cctgataaga cgcgacagcg tcgcatcagg cgttgattac
    16321 tggatgcagc ctacacgcgg actgataagc caggcgaaat aatgaattat catcagtact
    16381 atcctgtcga catcgtcaac ggccccggca ctcgctgcac cctgtttgtc tccgggtgtg
    16441 ttcatgaatg ccccggttgc tataacaaaa gcacctggcg ggtaaattcc ggtcagccat
    16501 ttaccaaagc aatggaagac cagatcatta acgatctgaa tgacactcgc atcaaacgcc
    16561 agggtatttc cctctccggc ggcgatccgc tgcatccgca aaacgtgccg gatattctga
    16621 aactggtaca acgcatccgc gccgaatgtc cgggtaaaga catctgggtg tggacaggct
    16681 ataaactcga cgaactcaac gctgcgcaaa tgcaggttgt tgatttgatt aacgtgctgg
    16741 tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc ggcagcagca
    16801 atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca ggcaacaggg
    16861 aaataaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc agttgctctg
    16921 cggcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg tcgtcaatct
    16981 gaccgaccag aatatcgaaa ccgtggcgga agtctttcat ttccgggctg tccggtgatt
    17041 tgccttcgag cttcggcggc gtcgcttttt gcgcatccag cgctgcggcg cgcatcttcg
    17101 ttaacgcgtc tttgacttgc gccgcgttat cggctttttc gaccactttt aaattgtcgt
    17161 tgagggtttc catattgtct tcaagatcag cggcaaacga cgccgaactg aataccaacg
    17221 aggagactgc aagaatagct aacaggcttt tacgcattgc tcacttcctt ttttatttac
    17281 acttcatcct tcacactgcc tcatcgacgg ctacgctcat tcgcccgagt cacgtactgc
    17341 gagtacgctc cccggtcctc attcactttc cgtctcgatg cagtgcgaat gattttgtgt
    17401 aattttttag tttatagcgc ggcaggtcgc gccagttttt tactgcccgg cgattttcat
    17461 ctccggcaac agcacagaac cacactgtat attactgcgt gtttcaatat cgttaccgac
    17521 ggtgacaata ttgcgccaca tatcttttaa attacctgcg atggtgattt cgctaaccgg
    17581 atactgaatt tcgccattct ctacccagaa gcccgctgca ccacgggaat aatcaccggt
    17641 aatggcactc acgccctggc ccatcagctc cgtcaccacc agcccggtgc ccatctcttt
    17701 gagcatttgt tcgaagctta gcccttgtcc ggcaatccgc cagttgtgaa taccgcccgc
    17761 atgtccggtg cttttcagcc ccagtttccg cgccgaatag ctggtcagca gccactgagt
    17821 caggatgcca tctttaataa tatcgcgacg ctcggtgcgc acaccttcgc tgtcgaatgg
    17881 cgtagaggcc agccctttca gcagatgcgg atgctcttca atggtcagcc agtccggcag
    17941 aatttgttta cccagcgaat ccagcaggaa ggtagattta cggtaaaccg atccacccgc
    18001 tatcgcccca accagatgcc caaaaagccc ggttgccact tcattggcaa aaatgaccgg
    18061 cgctttcatg gtggagagtt tacgcggtga cagacgcgat aaagtgcggc gagcacagtc
    18121 ggccccgacc cactctggcg tttgcagatc gctcatcgca cgaccaatgg tgtaggcgta
    18181 atcacgctcc atatcgccat tttcttcggc aattacacag ctggagagcg aatgacgcgt
    18241 agagcagtaa ccctgcaaca tgccgtggct gttgccaaaa actttgacac cgtagtggct
    18301 gttaaagctg ccaccttcgg tattggtgat gcgtttgtcc gcctgcaatg ctgcctgttc
    18361 tgcgcgggcc gccagttcaa tggcttcatc cggggaaact tccgcagggt ggaacaagtc
    18421 gagatccggt gcgtcaaagg ccagcagctc tttgtctgcc acgccggcac agggatctgg
    18481 cgaggtataa cgggcaatat ctagcgccgc ctgtacggtg cgggcaatgg cctgcgggct
    18541 taaatcggta gacgatgcgc tacctttgcg gttctggtga taaacagtga tccccaacgc
    18601 gccatcgcta ttgaattcaa cattctccac ttcaccataa cgcgtgctta cgctaatgcc
    18661 ggtggtcttg ctgacggcaa cttctgcacc gtccgatttg cctgaggcca actccagcgc
    18721 agtcgaaact gcttcttcca gaatcttgcg ctgcgcttca acttgagaga ttactttcat
    18781 tgcaagtgcc ataatgtaga gagtttctct gaagtctaac agagaaccgt ttttcagtgc
    18841 gcatcttaac tggtaacatt agcctctttt tttaaggagc ctgagatgac taagcagccc
    18901 gaagactggc tcgacgacgt tcccggtgat gacatcgaag acgaagacga tgaaattatc
    18961 tgggtcagta aaagtgaaat taaacgcgat gccgaggagc taaaacgcct tggcgcggaa
    19021 attgttgatc tggggaaaaa cgcgctggat aagatcccgt tagatgcgga tctgcgcgcg
    19081 gctattgagc tcgcccagcg tattaagatg gaaggtcgcc gccgccagtt gcagctcatc
    19141 ggtaagatgc tgcgccagcg cgatgttgag cctattcgtc aggcgctgga taagctgaaa
    19201 aaccgccaca accagcaagt ggtgttgttc cataaactgg aaaacctgcg cgatcgtctg
    19261 atcgagcaag gtgatgacgc catcgctgaa gtattgaatc tgtggcccga tgccgatcgt
    19321 cagcaactgc gtactctgat ccgtaacgcg aagaaagaga aagaagggaa taagccgccg
    19381 aaatccgcgc gccagatttt ccagtatctg cgtgagttag cggaaaacga agggtaattt
    19441 tcgttgttga tgtgagtatg gtttgccgga tgcggtgcac ctgtaggcct gataagacgc
    19501 atcaagcgtc gcatcaggca ctatgctcaa atgccggatg cggcgtgaac gccttatccg
    19561 gcctacacac actctatcag gctgaggctg tgaattactg cgcggcttcc gctttcttcg
    19621 ctaacccatc cagcagtttc tgatggatcc caccaaaacc gccgttactc atcaccagaa
    19681 tgtgatcgcc cggctgagcg gttttcacca ccatatctgc cagcgtatcc acatcgccgc
    19741 tccagtgtgc aggctgaacg caggcttctg ccacttctgc cacctgccac ggaatatgcg
    19801 ccggttgcag caggaagact tcatcggcac gacctaatga aggtgccaga tcgtctttgc
    19861 agatccccat tttcatggtg tttgagcgcg gttccagcac ggcaataatg cgcgccgtac
    19921 caccaacttt gccacgcagc gccgcaagcg ttgccagaat cgccgtcggg tgatgggcaa
    19981 aatcgtcata taccgtcaca ccattcgctt caccacgcaa ctccagacgg cgacgagcat
    20041 taataaacga acccagcgcg ttagcggcat ctgccggcgc tacaccaaca tggcgagccg
    20101 ccgcaatcgc catcaggcca ttgtgcatat tatgttcgcc taccagcgac catttcactt
    20161 cgcccacttt ttcgccatcc agcaagactt cccattcgga agcatcggtg gtcagctttt
    20221 tcgcctgcca gtgaccttgc tcgcccacca gctcctgctc gctccagcag cccatcgcca
    20281 tggtctgttt caggttgatg tcattttccg gccagataat acggccctgc cccggaacga
    20341 tacgcaccag atggtggaac tgtttctgga tcgctttcag gtcgtcaaag atatcggcgt
    20401 gatcgaactc aaggttgttg aggatcagcg tacgcgggca gtaatggaca aatttagagc
    20461 gtttgtcgaa gaaggcgcag tcatactcat ccgcttcgat aacaaagaag tcgctttcgc
    20521 ccagacgcgc cgaaacctca aagttccccg gcacaccgcc gatcacaaat cccggtttgt
    20581 aaccgcactg ttccagaatc caggttgcca ttcccgcggt ggtggttttg ccatgtgtac
    20641 cggcaacggc cagcacccag cggtcgcgca gcacaaaatc gtgcagccac tgcggacctg
    20701 acatataagg gatgtttttt tccagtaccg cttccacaca cggatttcca cgggtcatgg
    20761 cgttaccaat aatcaccaga tccggctgtg gatcgagctg gctggcatcg taaccctgaa
    20821 tcagttcaat gccttgcttc tcaagtaagg tgctcatcgg cggatacaca ttggcgtccg
    20881 aacccgttac ttcatggcct aactggcgcg ccagcatcgc cagaccgccc ataaacgtgc
    20941 cacaaattcc taaaatatga atgcgcatac gtcactatcc ttttttaatc tggcgctcat
    21001 tttacgcata tgtcgggcaa gtgagaaacg catttcagga taatccgtaa tttgctggcg
    21061 cgattcacct gagcgcaaca ttgggattat ttgttaagat tgttgcggtc gctttactcc
    21121 ataaacattg cagggaaagt tttatgaaaa cgttaggtga atttattgtc gaaaagcagc
    21181 acgagttttc tcatgctacc ggtgagctca ctgctttgct gtcggcaata aaactgggcg
    21241 ccaagattat ccatcgcgat atcaacaaag caggactggt tgatatcctg ggtgccagcg
    21301 gtgctgagaa cgtgcagggc gaggttcagc agaaactcga cctgttcgct aatgagaaac
    21361 tgaaagccgc actgaaagca cgcgatatcg ttgcgggcat tgcctctgaa gaagaagatg
    21421 agattgtcgt ctttgaaggc tgtgaacacg caaaatacgt ggtgctgatg gacccactgg
    21481 atggctcgtc caacatcgat gttaacgtct ctgtcggtac cattttctcc atctaccgcc
    21541 gcgttacgcc tgttggcacg ccggtaacgg aagaagattt cctccagcct ggtaacaaac
    21601 aggttgcggc aggttacgtg gtatacggct cctctactat gctggtttac accaccggat
    21661 gcggtgttca cgcctttact tatgatcctt cgctgggtgt tttctgcctg tgccaggaac
    21721 ggatgcgctt cccggagaaa ggcaaaacct actccatcaa cgaaggaaac tacattaagt
    21781 ttccgaacgg ggtgaagaag tacattaaat tctgccagga agaagataaa tccaccaacc
    21841 gcccgtatac ctcacgttat atcggttcac tggtcgcgga tttccaccgt aacctgctga
    21901 aaggcggtat ttatctctac ccaagcaccg ccagccaccc ggacggtaaa ctgcgtttac
    21961 tgtatgagtg caacccgatg gcattcctgg cggaacaagc aggcggtaaa gcgagcgatg
    22021 gcaaagagcg tattctggat atcatcccgg aaaccctgca ccagcgccgt tcattctttg
    22081 tcggcaacga ccatatggtt gaagatgtcg aacgctttat ccgtgagttc ccggacgcgt
    22141 aatctcctgc gcctctccac acggagaggc gtttttatcg ctgcgcaatg ttgactcttg
    22201 tcacaggcga actctgacga ttctcccaca gcaccgttaa tccacgctgt aatgctataa
    22261 aaataaacaa caaaataccg atggcgattt tcgtccacca tgagctcagc gtgccatcaa
    22321 agtttatgta agtctgaatc agtccctgaa tcgccacgcc aaaaagcgtc cctaataccg
    22381 ttccaacgcc accactcaaa agcgtaccgc caattaccac tgaggcgata gcatccagtt
    22441 ccacacctac gcccgccagc gcatatccgg cctgggtata aatcgagaag acaatccccg
    22501 ccagtgtcgc cagcccggta gagagcatat aaatgcgaat agtggtgctg cgagtggaaa
    22561 tccccatcag gttcgccgac gttgcgttgc cgccaatggc gtacacctga ttaccaaaac
    22621 gcgtacgatg cgcgaggaat atgccgataa ccaccaccgc cagcatcagc agccccatcg
    22681 cacttaagcg accgccgcca gggattttcc acgcaaggct tgagagcgtg tcataaacag
    22741 gatggtttat cgggatcgac tcttccgaaa cgagatagct gacgccacgc aaaaagaaca
    22801 ttcctgccaa agtaatgata aacgccggga tcttcagggc gtcaatcaga agccccataa
    22861 atgcgccgaa ggcacagccc atcaccaaca ccagcggaaa cgccagcagc ggcgagaggc
    22921 cgaaatcgcc aatcactttt gccagaaaca cgccggtaaa ggcgatcacc gaaccgacgg
    22981 agagatcgat cccaccggag aggatcacaa aggtcatgcc aacggcgatt atcccaagaa
    23041 aggcattatc ggtcaggata ttgcagatca ctcttgtgga agcaaaaccg ggaaactggg
    23101 tcagacagta gatataaccc aacacaaaga cgccgatggt gatcatcagt ggcaaattac
    23161 gttttatcac ggttacgtac ccctttaatc agactgataa agcgttgcga ctggacaatc
    23221 agcacgcaaa gcaccaccac cgctttcacc acctggttca tctccggcgg aaatccagaa
    23281 agcaaaattc cggtgttcat tccctgaata atcagcgccc ccaccaccga aagcagcagg
    23341 ttaaagcgcc cgcccatcag cgatccgccg ccaatcacca ccgcgagaat ggcgtccagc
    23401 tccagccata acccggcgtt attggcatcg gcaccgcgaa tatccgccgc cacgataatg
    23461 cccgcaatcg ccgcacatag cccgctcaac acgtaggtga gcatgacgat gattcgcgtg
    23521 tttaccccgg cattttttgc cgcccgaata ttgataccaa cggcttcgat aaacatcccc
    23581 agtgccgttt tgcgggtcaa cagccagaac aggagaagcg tcagcaccgc aataatgact
    23641 ggcgttggca ggaacaacag cgatccactg ccgaaccagg agagatccgg tgagttaaat
    23701 gtgacgatct gtccggaggt gatcagttgc gccacaccgc gcccggcgac catcaggatc
    23761 agggtggcga caaacggctg aattttgagg atcgctacca gtatgccgtt ccacaatcct
    23821 gccaggatgc cagtgcccag ggcgcttaac aaaacaatcg gcaggctgaa tcccgcgacc
    23881 gtcatcgcag ccgttgtggc tccggcgata gccatcaccg cccctacgga gagatcaatc
    23941 ccaccggtgg cgataaccag cgtcatgcca atcgccagta gcgcaacggg ggccgcacgg
    24001 ttaagaatgt cgatagggct accgaacaaa cgcccatcct gaagcaccac ctgccagaaa
    24061 tgcggggcca ccaggctatc gaccagcagc accagcaaca gtgccgccag ctgcggcatt
    24121 cctgttggcc agcgaaaacg ccttttcggc ggcgtagtgt ccgggagaga ttgaggcatc
    24181 acactattct ccttacgccg caatggcgtt catgatcgcc ggaacggaaa gctctgccag
    24241 cgggatctcc gccacctgtt tgcgatcgcg catgataatc acccggtcgg catagcccac
    24301 cagctcttcc agttcagagg agatcaccag cagcgccaga ccgtcagcgc acagcgtttc
    24361 aatcaggcgg atgatttcag cgtgggcacc aacatcaatg ccgcgggttg gctcgtcgag
    24421 gatcaaaaat tgcggacgag tcagtagcca acgtgaaagc aacacttttt gctgattgcc
    24481 gccagagaga aattcaatcg gttgttcagt tgaaggtgtg cgaatgccaa gctggcggat
    24541 aaagcgttcg gcaatctctt gctgttcttt gcgggaaatc ggtcgtagcc agccgcgctg
    24601 ggcctggaga gcgaggatga tattttcccg caccgaggcg gcggcgatga tgccatcggt
    24661 tttcctgtct tccgggcaga agccgatacc cagtaccgaa gcctgatgtg gcgatcgcag
    24721 ggtttgctgt ttgcctttga tcaacgccgt gccgctgtca gcaggtttga tcccgaagat
    24781 cacttcggcg gtttcggtac gtccggatcc cagcaagcct gccaggccaa cgatctcgcc
    24841 ggggcgtact tcgagatcaa acggtgcgat cgttcctttt ttgccgtaat ttttgaacgc
    24901 ggcaacgggt ttgtcgctca gcaatgttct cccggcacgc tgaagcgcgt gggtgtccag
    24961 ttcacgcccc agcatcattt ttaccagttc gatctgcggt agctcgcggg tttcccgaca
    25021 gcccacaaaa ctgccgttac gtaagacggt gatccgatcg ctaacctgat aaacctgatc
    25081 gagaaaatga gtgacgaaga tcaggctgac gccgcgatcg cgaagctgac gcatcaggcc
    25141 aaacagtaac tctacttcct gagtgtcgag actggcggtg ggttcatcga ggatcagcac
    25201 tttggcggag agatcgatag cccggcaaat ggcgacgatt tgctgcatcg cgactgaaaa
    25261 gcggttgagc ggttcgcgca cgtcgaggga gaaaccgtaa gatgccatca gttcggtggc
    25321 gcgcttttcc atctctttac ggcgtagaag gccgaaacgt ttgggttcgc gacctatgaa
    25381 tagattatca gcgaccgaca tattgggtag caggttgact tcctgataga cggtgccgat
    25441 gccgagttgt tgtgcgtggg cggtgttttt cggtgagata gcctggcctt ccagccagat
    25501 agtgccgcga tcggcgtggt atacaccggt taatgctttg attagcgtcg attttcccgc
    25561 cccgttttca ccgagcagcg ccatgatttc gccacggcgc aggctgaaat caacgttgtc
    25621 taacgctttg acaccgggga aaaatttact taatccttcg gtgcggagga tttcctggtg
    25681 ttggtcggtc gtcatggttt cccccggtgt aaagcgtgct cccagtaggc atgataagac
    25741 gcggtaagcg tcgcatcagg cttcagcgca caaccgccgg atgcggcgcg agcgccttat
    25801 ccggcctacg aatcggcatc aggcatagca accctcaata ccccatattt ttcttcttct
    25861 ctaactcttc ttttgcggta tcaggcagat aaagggtgga tttggttaac gtcagctttt
    25921 caggcatggt gccgtctttt ttatatttct ccagcgcgtc gaaggcggga cctgccatat
    25981 tcggcgtcag ttcaacactg gcgttcgctt cgccatcaat catcgctttg tagatgtccg
    26041 gcacaccgtc gatagagcct gtcaggatat ctttgcccgg tttcaggccc gcttctttaa
    26101 ttgcctgaat tgcaccaatc accatgtcat cgttatgggc gtaaaccatg cagatgtttt
    26161 tgccgttgtt ttccgctttg ataaagctct ccatcacttc tttgcctttg ctgcgggtga
    26221 agtcacctga ctgcgagcgg ataattttga tatttggcgc attcttaatg gcttcggcaa
    26281 agcctttctt acggtcaatg gcgacgctgg ccccaacggt gccctgcagc tccaccacgt
    26341 tgcatggttt gccattcact tcttttacca gccagtcacc aatcaacttg ccttcgagga
    26401 tgttgtcggc ggtgacagtg gtcatataga gagatttgtc tttcacatcg atggaacggt
    26461 caagcaagaa gaccgggatt tcggcatctt tcgcctcttt taataccggc tcccaaccgg
    26521 tcgctaccac tggagcgata aagatcgcat ccaccccttg cgcgacgaag gaacgtaccg
    26581 ctttaatctg gttttcctgc ttttgctgac catcggcaat tttcaacgta attccgcgct
    26641 tttcggcttc acttttcgcc acattggttt ctgcggcgcg ccagccggat tccgacccga
    26701 cctgcgaaaa tccaacggtt aatggagcgg ccaacgccat agacgacatg gctgccgaga
    26761 ctgcagagac tacaagtaag cgtttccaca tagggttgtc ctcgtcgggg taattattat
    26821 tggtgaaaag atgttcgcga aaaaactata gacaattcgt tatgtaacgg attgcgttac
    26881 atcacacttc aaaaaagtaa ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca
    26941 ctatgaaaaa tgcggctacg gttatggatt ttcctgctct gtataccgtc ttaaaactgg
    27001 cgaaaaagga aaatgaagac gaaaacaagc gaagacattc ggcgcgagtt ggctataata
    27061 ctcggcactt gtttgccaca tatttttaaa ggaaacagac atgagcttac tcaacgtccc
    27121 tgcgggtaaa gatctgccgg aagacatcta cgttgttatt gagatcccgg ctaacgcaga
    27181 tccaatcaaa tacgaaatcg acaaagagag cggcgcactg ttcgttgacc gcttcatgtc
    27241 caccgcgatg ttctatccgt gcaactacgg ttacatcaac cacactctgt ctctggacgg
    27301 tgacccggtt gacgtactgg tcccgactcc gtacccgctg cagccgggtt ctgtgatccg
    27361 ctgccgtccg gttggcgtac tgaaaatgac cgacgaagcc ggtgaagatg cgaaactgat
    27421 tgctgttccg cacaccaagc tgagcaaaga atacgatcac attaaagacg ttaacgatct
    27481 gccagagctg ctgaaagcgc aaatcgctca cttcttcgag cactacaaag acctcgaaaa
    27541 aggcaagtgg gttaaagttg aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt
    27601 tgcttccttc gagcgcgcca agaataaata agttcttctg gcgcaataac cctgaacacc
    27661 gggcttcggt tagtaagggt ttttttatgc ccgcaattta taaactctct actccactat
    27721 tattattttc agcagttgaa aggcttgaac ggtaagaaca agctaaactc gaccaccatt
    27781 ttgctgttca tagccacttg ctggaagtta gccggcctca ctcatactca ccgttcaaag
    27841 cgtgtccccg ttttacccct gtacatttgt taacgccagc agagtttttt atgcgattgc
    27901 agatatggaa agcggcccgg agagaaaagc aaaagatggg aaagttacaa aaaaaatcgc
    27961 gagatacatc gcataaaaca acgccacctt ttcggtggcg tttttatcgc ccacgcacca
    28021 ccagtgcctg gtccagctcg cgatacgctt caaccagttt ctccagtgaa acgcgactta
    28081 aaccgctggg atttggcagc acccaaatct gcgtcgaacc aatggtgaga gtttgtttcc
    28141 cccactgtgc accgcgctgg ctgaatccct gttcatatgc ttgtttgccc agaatcgcca
    28201 acgcctgcgg ctgataatct tcaatttttt caatcagctt acgcccgcct gcgtgtagct
    28261 cctgctttga aacttcattg gcttgcaccg ttggacggtc taccagtttg gtgacgccac
    28321 aacgataatc cagcagatgc tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg
    28381 cctgatatat caccttccag aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg
    28441 cggatgaaag cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat
    28501 cctcaaccat atttactcct ttctaacatc accaggaaaa gtataacgga ttgaaaatac
    28561 attgtttata aaaacagcag gcgcgcggta atggctggat tgcgacacgg agttacttta
    28621 taatccgcta ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg
    28681 tcgttggttc aaacccaaca ggggccacca ta
//
LOCUS       DAZGTJ010000067        28485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000067 DAZGTJ010000000
VERSION     DAZGTJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.067"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(128..613)
                     /gene="sixA"
                     /locus_tag="ACHK8E_003413"
     CDS             complement(128..613)
                     /gene="sixA"
                     /locus_tag="ACHK8E_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP0345633.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(816..2960)
                     /gene="fadJ"
                     /locus_tag="ACHK8E_003414"
     CDS             complement(816..2960)
                     /gene="fadJ"
                     /locus_tag="ACHK8E_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP0345634.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2960..4270)
                     /gene="fadI"
                     /locus_tag="ACHK8E_003415"
     CDS             complement(2960..4270)
                     /gene="fadI"
                     /locus_tag="ACHK8E_003415"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP0345635.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4451..4735)
                     /gene="yfcZ"
                     /locus_tag="ACHK8E_003416"
     CDS             complement(4451..4735)
                     /gene="yfcZ"
                     /locus_tag="ACHK8E_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP0345636.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5107..6447
                     /gene="fadL"
                     /locus_tag="ACHK8E_003417"
     CDS             5107..6447
                     /gene="fadL"
                     /locus_tag="ACHK8E_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP0345637.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            complement(6509..7264)
                     /gene="mlaA"
                     /locus_tag="ACHK8E_003418"
     CDS             complement(6509..7264)
                     /gene="mlaA"
                     /locus_tag="ACHK8E_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP0345638.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            7558..8490
                     /gene="yfdC"
                     /locus_tag="ACHK8E_003419"
     CDS             7558..8490
                     /gene="yfdC"
                     /locus_tag="ACHK8E_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP0345639.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            8566..8640
                     /locus_tag="ACHK8E_003420"
     tRNA            8566..8640
                     /locus_tag="ACHK8E_003420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8599..8601,aa:Arg,seq:cct)
     gene            8820..9002
                     /locus_tag="ACHK8E_003421"
     CDS             8820..9002
                     /locus_tag="ACHK8E_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0345640.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(9082..10329)
                     /locus_tag="ACHK8E_003422"
     CDS             complement(9082..10329)
                     /locus_tag="ACHK8E_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345641.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(10401..11315)
                     /locus_tag="ACHK8E_003423"
     CDS             complement(10401..11315)
                     /locus_tag="ACHK8E_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP0345642.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            11531..12964
                     /gene="cscA"
                     /locus_tag="ACHK8E_003424"
     CDS             11531..12964
                     /gene="cscA"
                     /locus_tag="ACHK8E_003424"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP0345643.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(12972..13967)
                     /locus_tag="ACHK8E_003425"
     CDS             complement(12972..13967)
                     /locus_tag="ACHK8E_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0345644.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            14210..14428
                     /locus_tag="ACHK8E_003426"
                     /pseudo
     CDS             14210..14428
                     /locus_tag="ACHK8E_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            14446..15777
                     /gene="dsdA"
                     /locus_tag="ACHK8E_003427"
     CDS             14446..15777
                     /gene="dsdA"
                     /locus_tag="ACHK8E_003427"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP0345645.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(15882..17420)
                     /gene="emrY"
                     /locus_tag="ACHK8E_003428"
     CDS             complement(15882..17420)
                     /gene="emrY"
                     /locus_tag="ACHK8E_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP0345646.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(17420..18583)
                     /gene="emrK"
                     /locus_tag="ACHK8E_003429"
     CDS             complement(17420..18583)
                     /gene="emrK"
                     /locus_tag="ACHK8E_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP0345647.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
     gene            18999..19613
                     /gene="evgA"
                     /locus_tag="ACHK8E_003430"
     CDS             18999..19613
                     /gene="evgA"
                     /locus_tag="ACHK8E_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP0345648.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            19618..23211
                     /gene="evgS"
                     /locus_tag="ACHK8E_003431"
     CDS             19618..23211
                     /gene="evgS"
                     /locus_tag="ACHK8E_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP0345649.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(23267..24412)
                     /gene="yfdE"
                     /locus_tag="ACHK8E_003432"
     CDS             complement(23267..24412)
                     /gene="yfdE"
                     /locus_tag="ACHK8E_003432"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP0345650.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(24486..25430)
                     /gene="yfdV"
                     /locus_tag="ACHK8E_003433"
     CDS             complement(24486..25430)
                     /gene="yfdV"
                     /locus_tag="ACHK8E_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP0345651.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(25500..27194)
                     /gene="oxc"
                     /locus_tag="ACHK8E_003434"
     CDS             complement(25500..27194)
                     /gene="oxc"
                     /locus_tag="ACHK8E_003434"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP0345652.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(27248..>28485)
                     /gene="frc"
                     /locus_tag="ACHK8E_003435"
     CDS             complement(27248..>28485)
                     /gene="frc"
                     /locus_tag="ACHK8E_003435"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP0345653.1"
                     /translation="QGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQL
                     RDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGF
                     TWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAA
                     ALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGY
                     LEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTC
                     KAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVL
                     SMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVL
                     QELGYSDDEIAAMKQNHAI"
BASE COUNT         7908 a         6950 c         6381 g         7246 t
ORIGIN      
        1 cgtataaaat aaagaccatt cgatcacaag cggatcattg ttagtacaat aagattgcat
       61 aaattcaaca ttgccagaaa aaaataaagg cccgatagat tacaccagcg ggccttgtgc
      121 atgcgtatta aatcgctttt gccatcttca gattacacgg gctcatctgc cagttgaatg
      181 tgccgtttcc gctttcatca agcgttacgc tggcaatggc cgaggtggta aacataggcg
      241 gcgtttcgcc cggacataac tcggcaacca gatacccgac taagggtaag tgggagatca
      301 ccagtaccga agcaacacct tcattggtca acgcctgcaa ataggcactg accagaccaa
      361 catcgccgca gggcgttaac tccggcagaa cttctgcact ggaaggcagg ttcagacaat
      421 cccctacttc ttcaagtgtt tgctcggctc gcaggaacgg gctcaccaga acgcgttcga
      481 tttccacttt ttgacctttc agccagttcg ccatcaggcg agattcgtca caaccgttag
      541 tggtcagagg acgaaccgaa tcactggcgg catcgagggc tgcgtcgccg tgacgcatga
      601 taaaaacttg catattgcac cgcttttgtt aaccagattc gcccgttttc tttcatctga
      661 aaccagagaa agaaaacggt ggccggcatt gtgccttatc cattcaccga atgaaacgct
      721 gttttttacc tcaatggcgt aagtatagtc aatatgcgtt tacattttga gcaacactcc
      781 gccattcagc gcggattcat atagctttga ccttcttatt gcaggtcagt tgcagttgtt
      841 ttccaaaaac tttccccacg cttgctcatc tcgactaaac gctcgcaagg ggtaaaacgg
      901 gaaccatact gcgtggccag tcgttgcatt attgcaacca cttcacccgc accgagagaa
      961 tcgatgtagc ggaacggacc accgagaaat ggcggaaaac caatgccaaa taccgcgcca
     1021 atatctccgt cacgcacgct acggataacc tgctcgtcca gacaacgtac tgcttcattc
     1081 agcatcaaca tcacacaccg ttcagctacc tgcggtgcgg agagtcgccc ctgcccttgt
     1141 gtgccaatga gcgggtaaat ggcgggatcg acctgttttt tgcttttacg ccctttctga
     1201 ccataaagat agaaaccccg gccatttttt ctgcctttgc gatcgtcgtt caaaattgaa
     1261 gaaacaacat ttgcaggcgc gctaaaacgt tctccataag cggcttccag tacaggaata
     1321 attttagtcc cggtgtcgat tcctacctca tccaaaagtt ggattgggcc taccggaaaa
     1381 ccaaatttca ccagcgcggt atcaatgtgc tctacccgtt caccttcggt caacatgcgg
     1441 atagcttcat taatgtaagg cgctaagatg cgattgacgt aaaacccggc tttgtcacgc
     1501 acgacaattg gcgttttgcc ctgttttttc gccagtttta ctgtggtggc gatggtttgc
     1561 gccgatgtac cggcatgggg aataatttct accaacggca ttttttccac cggactgaag
     1621 aaatgcaggc caataacttg ctcaggtcgc gcggcgtgag cggcgatatc gccaatcggt
     1681 aaagatgacg tgttcgaagc aaagatggta tgagcggcgc aattttgctc aacttccgcc
     1741 accatttgtt gttttaattc gagattttca aacaccgctt caataatcag atcgcgatgg
     1801 gcaaagccgc aatagtccgt cgttccggag attaatgcca gctgtctgtc acgttcgctg
     1861 gctttgagat gacgacggcg aactttgccc tccagctgat cccaactgta cttcagcgca
     1921 tgatttatgc cctgcgggtt gatatcttta attctgaccg gaagccccgc tttacaagca
     1981 gtgacataag caataccgcc gcccatcaag ccaccaccta aaattcccac gctgtttaat
     2041 ggcgcaggcg gcgcatcact gccaggatct ttcttcacgt ccgtactggc aaaaaagata
     2101 ctacgcagcg cctgcgattg cggcgtcatc accagttcgc caaaagcacg ggcttcagcg
     2161 tcatagccgc cactggtgcc ctgcgctaat cccgtttcga caacctccag gatgcgttct
     2221 gtcgccggat agttaccttg agttttgtgt tctgttttct taccgaccat tttgaacagc
     2281 agcgcacgac ctaacggccc cgccagaata cgctcgcgta caggtagagg gcgtgaagat
     2341 gggcgatcct gctttgccag ctcaacagcg gcttccagta aaatggattg cggaacgacg
     2401 tcatccacca gccccagctt taatgcctgt ttcgcccgaa gttgttttcc agtgaggatc
     2461 atctccaatg ctgtgctgac acctatcagg cgcggtaaac gctgggtgcc gcctgaaccg
     2521 ggtaataatc caagttgtac ttcaggcaaa ccgagcaccg ttttaggatc gtcagtacac
     2581 atgcgaccgt ggcacgccag cgccaactcc agcccaccgc ccaggcaagc gccatgaata
     2641 gccgcgataa ccggaacggg caaagcatga atctccgcca tcaactgttg gccctgccgc
     2701 gccagtgctt ccgcttcttg cgccgttttg cagttggcga tcatgttgat gtctgcgcca
     2761 gcaatgaagt tgtctggttt cgcggagata aacaccacgc cgcgcaactc tttgttttca
     2821 cggagttgct taataatggc gcgcacctgc gaggcaaact ccgccttcag ggtattcatt
     2881 ttctcacccg gtacgtcgat ggtgataaca gcaatgttgt ccagacggac attaagggta
     2941 aacgctgatg ccatttccat tattccgcct ccacaaccat tgccgcgcca agcccacccg
     3001 cagcacaggc ggtcactaaa ccaaatccac cgccgcgacg gcgaagttca tgcaatgtct
     3061 gggtaatcat ccgcgcgccg gtcgccgcga agggatgccc gtaagcaatc gaaccgccaa
     3121 gcacgttaaa tttgctatcg tccatttcgc cagtggcatg tgcacgcccc agtacatcac
     3181 gagcaaaacg ttcgctaccc agcaactgaa tattcgccag tgtctgagct gcaaaggctt
     3241 cgtgcatatc gagcaatgtc agatcgctca ttgtcaaacc ggcacgttcc agcgccagcg
     3301 gcgttgacca ggccggaccg agcaacatgt cctgccagac atctatcgca gtaaatgcgt
     3361 aactgcgcag ataccccagc ggtaccagcc ctaattcttt cgcccgcgat tcggtcatca
     3421 ggatcaccgc cgctgcgcca tcggtcagcg gcgtactgtt tgctgccgtt accgtcccgt
     3481 gtttacgatc aaacgccgga cgcaactttg cgtaatcagc aagcgaggaa ttaccgcgaa
     3541 tattgttgtc ttcggcaagc ggttgtttat aaggagggat aaaggcagtc atcacctctt
     3601 ctttgagttt accgtctgac cacgcctgag cggctcgctg atgcgaacgg tgcgctaatg
     3661 catcttgctg ttcgcgagta ataccgtagg ttttcgccat ttgttcagcg gtgtcgccca
     3721 tccgcaagcc agtagagtat tccgccactg ccggaggcac cggcattaag tcgcgcaaac
     3781 gcaggcgaga gaagagtttc aggcgctggc tcatggttcg agctttgttg acatcaacca
     3841 gcacgcgcgc cagtttttta ctgacgccaa ttggcaatac ggaagaggaa tcggccccac
     3901 cggcaatccc cgcgcgaata gttcccgcca tcaggctttc tgcgacgttt gcaatcgcct
     3961 ggaaactggt agcgcaagcg cggctgacgc tgtaagcatc ggtatgcaca ttcattccgg
     4021 taccgagaac aatttcacgc gcaatgttgg gggcttcagg catttgtacg acctgaccaa
     4081 aaaccagttg ttcaatcact tcggcgggga tctcgctgcg tgccagcagt tcgcctacca
     4141 ccatcttccc caaatcaaca gcaggaatgc catgaaaagc cgtcgcctgg cgggcaaaag
     4201 gcgtacgtaa accgctaaca atggcgatac gatcgccctg gcgggtaacc agcggtaaaa
     4261 cctgacccat aacactcccc tgtaaaataa taaataaagt ggtctgacct gatcatagtc
     4321 ttaaccattt ttttacattt agccaagtgg agaaaaggga aagtgggagc tatgacacag
     4381 agagaaaagt agaagagaaa agaaaacgcc cctgccagca actgacaggg gcgtatgtac
     4441 ccgagaggaa ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag
     4501 tgaaatcgat atccaggcgc acaccgtcag actcttcagt gaaagtcggg gtgattttgc
     4561 acggttcgga ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt
     4621 ctgcgcggtt tgcaaacaca cggctgtaag acgcggtgca gtcggagttg tccataatgg
     4681 tgccaacatc catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg
     4741 atttcctctg tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg
     4801 gaaagtgctg ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta
     4861 aaaatgatct aaaacaaaat tcacccgaat ccatgagtgc gccacctccc aattttgcca
     4921 gctggatcgc gtttcttaga tcatatctga aaaaagatag aaacatactt gcaacattcc
     4981 agctggtccg acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta
     5041 cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt
     5101 atggtcatga gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt
     5161 atctccaccc aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg
     5221 ggccgggctt attcagggga aggtgcaatt gccgatgatg caggtaacgt tagccgtaac
     5281 cccgcattga ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgat
     5341 ccggatgtaa atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc
     5401 gcgcctacgg catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt
     5461 tggggggctt caattacctc taactatggt ctggcgacag agtttaacga tacttatgca
     5521 ggcggttctg tcggtggtac caccgacctc gaaaccatga acctgaactt aagcggtgca
     5581 tatcgcttaa ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg
     5641 aaaattgaac gtttcgcagg cgatctgggg caactggtgg ctggtcagat taggcaatct
     5701 cctgcttggc tgactcctca ggggcaagca ttagcagcta ccgccaacgg catcgacagt
     5761 aataccaaaa tcgctcacct gaacggtaac cagtggggct ttggctggaa cgccggtatc
     5821 ctgtatgaac tggataaaaa caaccgctac gcattgacct accgctctga agtgaaaatt
     5881 gacttcaagg gtaactacag cagcgatctt aacccggctt ttaataacta cggtttgcca
     5941 attcctaccg cgacaggtgg cgcaacgcag tcgggttatc tgacgctgaa cctgcctgaa
     6001 atgtgggaag tgtcgggtta taaccgtgtt gatccgcagt gggcaattca ctatagcctg
     6061 gcttacacca gctggagtca gttccagcag ctgaaagcga cctcaacgag tggcgacacg
     6121 ctgttccaga aacatgaagg ctttaaagat gcttaccgca tcgcgttggg aaccacttat
     6181 tactacgatg ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg
     6241 gcacagaatc gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg
     6301 acttacgcat ttaataaaga tgcttcagtc gatgttggtg tttcttatat gcacggtcag
     6361 agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc
     6421 ggtactaact ttaactacgc gttctgataa cgcgttcgct taataagaaa aaaggtgagt
     6481 tttgcgactc accttttttg tttgtttctt attcagaatc gatatctttt aaatcatcct
     6541 gaatcgcttg cgcgttcgga ttttcctgcg gtttgagttc gccgccatta gcgatgaaat
     6601 catgacgctg gaagtacgct tcgcgcacca taatataagg atcggacgac tgacgcagca
     6661 gaccatcgga atccaacaac tgcgcacgag tttctatccc ttcaagcgtc catttaccca
     6721 cagacatcgg ccaggtcagc caggaaagaa ccgggtaaag accatccgcc atatcaccac
     6781 cgtcatcacg cagcgtgaag ctaccgtaga acggtaactg aacgtatggc ccataaccca
     6841 caccataatg accaagcgta ctaccgaagc ggtgaggttc agtccgttgc agtttcgggt
     6901 tcgccatccc tgcaacatca ataaagccgc ccatccccaa aatggtgttc aggaaaaagc
     6961 gggtaaagtg gaccatcccc tgataagggt cgccctgcaa gaagtagtta accatcaccg
     7021 caggttcttc aaggttgcca gtaaagttgc tcaaaccgtt acgcgccggt tgcggaacat
     7081 aatcacgcca ggcgacagcg accggtcgaa caatatacgg gtctaataca ttgaaattga
     7141 agttgtacat ggtgcggttg aacccttcta gcgggtcaga acgcccttgc tgatctgtac
     7201 cggaactcgc acaccccacc agaagcgtag ttcccagagc aagcgccgac aggcgaagct
     7261 tcataaatgt ctccctgttt ttttatggct tatgcagttt gccatccatg acggaacgat
     7321 accgtatccg cctgtttagg tgtgggcgat tgtaacagca cgtcaactga tgtccagacg
     7381 ccctgatttg ctgatttgat catagcctgg taatcgccgc cctgtaggct acttgattct
     7441 atagaaacag aaaaaggcaa acgcagcctt ttctacattt tcagagtaac tcccgccagt
     7501 tgcgagcaaa aaagccgcta cgctttagct atacgtgcta attcaagaga agagaccatg
     7561 gacaacgaca aaattgatca acacagcgac gaaattgaag ttgagagcga agaaaaagag
     7621 cgcggcaaaa caatagaaat agatgaagat cgactcccct cccgggcgat ggcaattcat
     7681 gagcatatcc gccaggatgg tgaaaaagag ctggaacgcg acgcaatggc actactgtgg
     7741 tcagccattg cggcgggtct gtcgatgggc gcttcgctac tggcaaaagg gatatttcat
     7801 gtcgaactgg aaggcgtgcc gggcagcttc ttgctggaga atctcggtta tacctttggt
     7861 tttattatcg tcattatggc ccgccagcaa ttatttaccg aaaacaccgt gactgcggta
     7921 ctacccgtca tgcaaaaacc gacaatgagc aacgttggct tacttatgcg gttatggggc
     7981 gtcgtgctgc tgggtaatat tctcgggaca ggtattgcgg cgtgggcatt tgaatatatg
     8041 ccaatcttca atgaagaaac tcgcgatgca tttgtcaaaa tcggcatgga tgtgatgaag
     8101 aacaccccca gcgagatgtt tgccaacgcg atcatttccg gctggctgat cgccactatg
     8161 gtttggatgt ttcctgcagc gggtgcggca aagattgtgg tgattatatt gatgacctgg
     8221 cttattgccc tgggtgacac cactcacatc gttgtcggtt ctgttgaaat cctctatctg
     8281 gtgtttaacg gtacgctgca ctggagcgat ttcatctggc ccttcgcact acctacttta
     8341 gcggggaaca tctgcggcgg cacctttatc ttcgcgttaa tgagtcatgc acagatccgt
     8401 aacgacatga gcaacaagcg caaagcagaa gcacgccaaa aagcagaacg tgcggaaaac
     8461 attaagaaaa atgataaaaa cccggcataa atggcgaggg tttaagcaat cgagcggcag
     8521 cgtacttacc ccgcagtcca ttagcgggta tactcatgcc gcattgtcct cttagttaaa
     8581 tggatataac gagcccctcc taagggctaa ttgcaggttc gattcctgca ggggacacca
     8641 gatacccttc aaaccaaatc taccttcacc ccgtaaaaga tgggtttggc agcacacttg
     8701 ccctatatct actcattttt actgcaacag gttgaaatct cagcactgtc agaaagcgct
     8761 gatgactaaa cagccctggg ccgggcgatg taaccatcat acagaatcct gatagcgaaa
     8821 tatggcgtga ctcgatactt cactctgcaa tgcattcctt gatgaattcg caggcccgtg
     8881 atacacggga cacgtcgctg aatgacgaca atgtcctgga aatcagcgaa ccgcgtatcc
     8941 ggagtacatt tgagcgactg taccagaaca tgaatgaggc gtttggatta ggtgattatt
     9001 agcaggggta agcatttttg tattattatt ttccggttga gggatatgga gatatcgaca
     9061 acaaccggaa aaagtttacg tctatattgc tgaaggtaca ggcgtttcca taactatttg
     9121 ctcgcgtttt ttactcagga agaaaatgcc aaatagcaac atcaggcaga caatacccga
     9181 aattgcgaag aaaactgtct ggtagcctgc gtggtcaaag agtatcccag tcggcgttga
     9241 aagcagcaca atcccaagcg aactggcaat ttgaaaacca atcagaaaga tcgtcgacga
     9301 caggcgctta tcaaagtttg ccacgctgta tttgaagacg gatatgacac aaagtggaac
     9361 ctcaatggca tgtaacagct tcactaatga aataatccag gggttaacga acagcgcgca
     9421 ggaaaggata cgcaacgcca taatcacaac accgataagt aatgcatttt ttggccctac
     9481 ccgattcaca aagaaaggaa taatcgccat gcacagcgct tcgagtacca cctggaatga
     9541 gttgagataa ccatacaggc gcgttcctac atcgtgtgat tcgaataaac ctgcataaaa
     9601 gacaggaaag agttgttgat caaaaatgtt atagaaagac cacgtcccca caataaatat
     9661 gacaaaaacc cagaagtttc gatccttgaa aactgcgata aaatcctctt tttttacccc
     9721 tcccgcatcc gccgctatgc actggtgatc cttatcttta aaacgcatgt tgatcatcat
     9781 aaatacagcg ccaaatagcg agaccaacca gaagttgata tggggactga tactaaaaaa
     9841 tataccagca aagaacgcgc caatagcata gccaaaagat ccccaggcgc gcgctgttcc
     9901 atattcgaaa tgaaaatttc gcgccatttt ttcggtgaag ctgtcaagca aaccgcatcc
     9961 cgccagatac cccaggccaa aaaagagcgc ccccagaatt agacctacag aaaaattgct
    10021 ttgcagtaac ggttcataaa cgtaaatcat aaacggtccg gtcaagacca gaatgaaact
    10081 catacaccag atgagcggtt tcttcagacc gagtttatcc tgaacgatgc cgtagaacat
    10141 cataaataga atgctggtaa actggttgac cgaataaagt gtacctaatt ccgtccctgt
    10201 taatcctaga tgtcctttca gccaaatagc gtataacgac caccacagcg accaggaaat
    10261 aaaaaagaga aatgagtaac tggatgcaaa acgatagtac gcatttctga atggaatatt
    10321 cagtgccata attacctgcc tgtcgttaaa aaattcatgt cctatttaga gataagagcg
    10381 gcctcgccgt ttacttctca ctattccagt tcttgtcgac atggcagcgc tgtcatcgcc
    10441 cctttcgccg ttactgcaag cgctccgcaa cgttgagcga gatcgataat tcgtcgcatt
    10501 tctctttcat ctgtagataa tcccgtagag gacagacctg tgagtaaccc ggcaacgaac
    10561 gcatctcccg cccccgtgct atcgacacaa ttcacagaca ttccagcaaa atggtgaact
    10621 tgtcctcgat aacagaccac caccccttct gcacctttag tcaccaacag catggcgatc
    10681 tcatactctt ttgccagggc gcatatatcc caatcgttct gtgtttttcc actgataagt
    10741 cgccattctt cttccgagag cttgacgaca tccgccagtt gtagcgcctg ccgcaaacac
    10801 aagcggagca aatgctcgtc ttgccataga tcttcacgaa tattgggatc gaagctgaca
    10861 aaacctccgg catgccggat cgccgtcatc gcagtaaatg cgctggtacg cgaaggctcg
    10921 gcagacaacg caattgaaca gagatgtaac cattcgccat gtcgccagca gggcaagtct
    10981 gtcgtctcta aaaaaagatc ggcactgggg cggaccataa acgtaaatga acgttctcct
    11041 tgatcgttca gatcgacaag caccgtggat gtccggtgcc attcatcttg cttcagatac
    11101 gtgatatcga caccctcagt tagcagcgtt ctttgcatta acgcaccaaa aggatcatca
    11161 ccgacccgac ctataaaccc acttgttccg cctaatctgg cgattcccac cgcaacgtta
    11221 gctggcgcgc cgccaggaca aggtagtagc cgcccgtctg attctggcaa gagatctacg
    11281 accgcatccc ctaaaaccca tactttggct gacatttttt tcccttaaat tcatctgact
    11341 tacgcatagt gataaacctc tttttcgcaa aatcgtcatg gatttactaa aacatgcata
    11401 ttcgatcaca aaacgtcata gttaacgtta acatttgtga tattcatcgc atttatgaaa
    11461 gtaagggact ttatttttat aaaagttaac gttaacaatt cacccaattt gcttaaccag
    11521 gatgataaaa atgacgcaat ctcgattaca tgcggcgcaa aacgcactag caaaacttca
    11581 cgagcgccga ggtaacactt tctatcccca ttttcacctc gcgcctcccg ccgggtggat
    11641 gaacgatcca aacggcctaa tctggtttaa cgatcgttat cacgcgtttt atcaacatca
    11701 cccgatgagc gaacactggg ggccaatgca ctggggacat gccaccagcg acgatatgat
    11761 ccactggcag catgagccta ttgcgctagc gccaggagac gagaatgaca aagacgggtg
    11821 tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt
    11881 ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag
    11941 tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag aaggcatcat
    12001 gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc
    12061 gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg
    12121 gactttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc
    12181 ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc
    12241 cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc
    12301 aggacgactt tttgcacaat ccgggcattt tactgaactt gatagcgggc atgactttta
    12361 tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat
    12421 gtgggaatcg ccaatgccct caaaacgtga aggctgggca ggctgcatga cgctggcgcg
    12481 cgagctatca gagagcaatg gcaaacttct acaacgcccg gtacacgaag ctgagtcgtt
    12541 acgccagcag catcaatcta tctctccccg cacaatcagc aataaatatg ttttgcagga
    12601 aaacgcgcaa gcagttgaga ttcagctgca gtgggcgttg aagaacagtg atgccgaaca
    12661 ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact
    12721 tgttttgtgg cggtattacc cacacgagaa tttagatggc taccgtagta ttcccctccc
    12781 gcagggtgac atgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat
    12841 taacgacggg gaggcggtga tgagtagccg aatatatccg cagccagaag aacgggaact
    12901 gtcgctctat gcctcccacg gagtggctgt gctgcaacat ggagcactct ggcaactggg
    12961 ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt
    13021 cccccttcac gatcttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga
    13081 agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag
    13141 cccattacgg cgacatcctg cgggatacgt acccccttcg ccaggagaac ctgataagcg
    13201 acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg
    13261 aagtggtcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga
    13321 aattgtttca cctcagccag atctcgtcca gcatcacgcc aggcctgctc aaatccctgc
    13381 cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataacgg ttgacgatag
    13441 cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa
    13501 ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga
    13561 gactcaggca gcgagatatg tcgcagcccc attgtagtat agataatgcc atccggacgg
    13621 tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa
    13681 aaactattcc agccaaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag
    13741 aaaggagtgg tggccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga
    13801 gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat
    13861 acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc
    13921 atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt
    13981 gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa cgatattctc
    14041 aacgccctat cacccgacac ggcaatacaa taaaaaataa caataattcc cggacaattg
    14101 tacccaattc cgcctctgtt ctcgccaacg agtaaccatg taggcaagta ggtaaaacat
    14161 gccaactggc atcgccaacc tggagaagaa aatataaata tggatcattg tgctgccgct
    14221 gtatcccgac atcagtccgg aaattattgc gattgctatc ggttcaggtg cgattggctg
    14281 cacgatcgtt acggactcgc ttttctggct ggtgaagcaa tattgcggcg ctacgctcaa
    14341 tgaaacattt aaatactata cgacagcgac atttatcgct tcagtcgtcg ctctggcggg
    14401 cacattcctg ctgtcattta tcatctaagc gcaaagagac gtactatgga aaacgctaaa
    14461 atgaactcgc tcatcgccca gtatccgttg gtaaaggatc tggttgctct taaagaaacc
    14521 acctggttta atcctggcac gacctcattg gctgaaggtt taccttatgt tggcctgacc
    14581 gaacaggatg ttcaggacgc ccatgcgcgc ttatcccgtt ttgcacccta tctggcaaaa
    14641 gcatttcctg aaactgctgc tactgggggg attattgaat cagaactggt tgccattccg
    14701 gctatgcaaa aacggctgga aaaggaatat caacaaccga tcagcgggca actgctactg
    14761 aaaaaagata gccatttgcc catttccggc tccataaaag cacgcggcgg gatttatgaa
    14821 gtcctggcac atgcagaaaa actggctctg gaagcggggt tgctgacgct tgaagatgac
    14881 tacagcaaac tgctttctcc ggagtttaaa cagttcttta gtcaatacag cattgctgtg
    14941 ggctcaaccg gaaatctggg gttatcaatc ggcattatga gcgcccgcat tggctttaag
    15001 gtgacagtac atatgtctgc tgatgcccgg gcatggaaaa aagcgaaact gcgcagccat
    15061 ggcgttacgg tcgtggaata tgagcaagat tatggtgttg cagtcgagga aggacgtaaa
    15121 gcagcgcaat ctgacccgaa ctgtttcttt attgatgacg agaattcccg cacgttgttc
    15181 cttgggtatt ccgtcgctgg ccagcgtctt aaagcgcaat ttgccgagca aggtcgtatt
    15241 gtcgatgctg ataaccctct gtttgtctat ctgccgtgtg gtgttggcgg tggtcctggt
    15301 ggcgtcgcat tcgggcttaa actggcattt ggcgatcatg ttcactgctt ttttgccgaa
    15361 ccaacgcact ccccttgtat gctgttaggc gtccatacag gattacacga tcagatttct
    15421 gttcaggata ttggtatcga taaccttacc gcagcggatg gccttgcagt tggtcgcgca
    15481 tcaggctttg tcgggcgggc aatggagcgt ttactggatg gcttctatac ccttagcgat
    15541 caaaccatgt atgacatgct tggctggctg acgcaggaag aaggtattcg tcttgaacct
    15601 tcggcactgg cgggtatggc cggacctcag cgcgtgtgtg catcagtaag ttaccaacag
    15661 atgcacggtt tcagcgcaga acaactgcat aatgccactc atctggtgtg ggcgacggga
    15721 ggtggaatgg tgccggaaga agagatggag caatatctgg caaaaggccg ttcataacgt
    15781 ttcaacgcag catcgccatc ctttccctgg gtgagcgatg ctgccgatgg cgcagactta
    15841 agatccccgg tcttacctgc tataattctc tctttatacc atcacccaac gcctttcgct
    15901 gtaaacggcg gtttcgcaaa ccaaaccaat accgtaagta agataaaagc aatagccgcc
    15961 atacggaaaa tctcatttgc agaaattgaa agtgactgct gagttatttc attattaatt
    16021 tcattaagaa ctcctgaaag cgaaccatag tatttatcca taatttgtga cgatgaatta
    16081 aatacggggt taaattgatc gatggttgct gtcaactgac tatggtgtaa cgattcgcgt
    16141 cgtccccaca gcgtcattgt caacgacgta ccaactgatc ctgacaaggt acgaaaaaaa
    16201 ttactcatac tcgaggcatt ggcaaattta ttatctggca accctgaaaa cgaaatcgtt
    16261 gttaagggta aaaagaaaca ggcaacggcg aatccctgga aaaactgtgg caaaatgatg
    16321 cctgtaaaat caatcgttgg cataaatgtc acagaacgcc agtaatagca aaccgcatac
    16381 atcaaaaaac taaatgtcac caacaaccgc atatcaattt tgttgccata acgtcctatc
    16441 aaaggtgata ttaatagcgg catgatgccg atgggcgcat aggcaagtcc ggcccatatc
    16501 gcattatacc ccatcgtttc ctggagtaac tgcggcataa ggactatcgc cccagagtaa
    16561 aataaatacg cgcatgtgat actcacaata ccaatggtga aattacggga cttaaacaaa
    16621 ctgagatcaa gaatcggatt ctctgaggtc gactcccaaa tgactaaaga aatcagagaa
    16681 ataactgaca ctactgttag tattattatt gtactcgagt tgaaccaatc cagatcgcgc
    16741 cctttatcaa gcattatctg caagccacca acaccgagca ctaacagggt cagtcctggt
    16801 agattcattt tgaccggtga agtctcagtt tctcttcctt taagtaaggt taagcataat
    16861 gtcagaacga taatccccat agggacattg attaaaaata tccaacccca gctaaagtta
    16921 tcgcaaatat agccgcccaa tatcggccca catatcggag cgataattac ggtcattgac
    16981 cataatgcca aagcaaatgt tcttttttct ggcggataat tccttaataa taaactctgt
    17041 gacagtggaa ttaacggccc cgccattaag ccctgaacga ctctaaaaaa tatcagcaca
    17101 tcaagattgg tcgataggct acacattaat gaagatagtg aaaaaaaggt tactgaaagt
    17161 aaaaataatc ttaattcgcc tattctttgt gccagcctgc cggtaacagg gatcgcaatg
    17221 gcatttgcta cgccaaacga agtgataacc caggtgcctt cgtctgttga tgctcccaga
    17281 aagccagata ttgtcggtat tgcgacgtta gaaatagtgg aatccaacat ttgcataaac
    17341 gtcgctaagg acaatgcaat agtaacgcac cataacgtcc caccggttaa tggtgccgga
    17401 gttgatttag tgattgccat taaagttgtc cattatgcga aataatgttg ctaatttctt
    17461 tttctatcgg agtagtatcg ataactaaag ccttactggt ataagcaggc atggaggtca
    17521 cggttgaagc cagctcaggc atctcagcaa tgtcttcgtc cttcgtatca atagttgcag
    17581 tcatcgataa accaatacgt aagggatgtt ccatgagttc ttttggatca agagaaactt
    17641 caaccggtac acgctgaacg attttgatcc agttccctgt cgcattttgt gcaggtaata
    17701 aggagaacgc attgccggtt cccatattga ttcctgtcac ccgaccatga aacacaacat
    17761 tttcaccata aagatcgctg ataatattga ccgattgacc aatccgtaca tccgtgagtt
    17821 gtgtttcttt aaagttggca ttaacccaca tttgacgtgc cggtacgaca gccattaacg
    17881 attgtccggg actcactgtt tcgccgacct gaacacttcg ctgggcaata tagccggtaa
    17941 caggactctt aatatccgta cgtttaagcg ctaaccaggc ttctttggtt gcatctgccg
    18001 cttcaacgac ttgtggctga cggtttaatg gtgtgttcat tactaaagct ttattcgctt
    18061 tataagcctg gatagcggca ttcaatgccg ctttgctact tattaacgta tctttggtat
    18121 gctccagcgt ttcttttgaa ataaccccct gcttcgctaa aggcacacga cggttataat
    18181 cttctaaaga ttgttgatac tgaatacgtg ctgaagcgac ttcggcactg tattgtttat
    18241 cctgtaagta cagtttattc gtttgccgaa caatatttgc cagattattt ttagctttat
    18301 tgagtgcgat agtggcatca gtcttatcca gtgagactaa aatgtcaccc tgtcgaacgt
    18361 agttcgtatc tttatgatta acgacagtga cgctgcctga aacttgtgca gaaattggat
    18421 ctgcattccc cgtgacatag gcgtcatctg tactaatcat gtcttctaat tccattgacc
    18481 aataggcata ggcacctgaa aacgcaataa ataaaactac cgccaataaa gaaaagtatt
    18541 ttcttctgtt agaatgtttt ttatttgaat taatctgttc cacttttatc tctcatttcg
    18601 catagatgaa atttatgaat tgaatgaaaa gagtattcat tcttcatatc aaacacagtg
    18661 catgtgttgc acattatccc aggaacgttg gaggcctaat ttaaatgagg tgcaaaaaaa
    18721 acaacagagg actaaaccgt ggcttttgca atacaattct tacgcatgta ggattagtga
    18781 gaagacttat agtgctaatg tgaaactata aatcatcggt acaatccctg attttattgt
    18841 tgacatttca tttatgccga ctatttatat ggtatacttg ttgaattatc ttaaaggaag
    18901 ctcaaatttt cttattttga ttgagaaaat gagatgatgc cttatgtctg tattactaca
    18961 gggagaaggg aaatgcttca ttgcaaaggg aataatctat gaacgcaata attattgatg
    19021 accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat attgaaatct
    19081 tagcggagtt gactgaaggg ggaagtgccg ttcagcgggt ggaaacactt aaacctgata
    19141 tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta gaaacgctga
    19201 ggaagcgcca atatagcgga attattatta tcgtctccgc taaaaatgac catttttacg
    19261 ggaaacattg tgctgatgct ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca
    19321 atatcattgc agctattgaa gctgcaaaaa atggctactg ctatttcccc ttctctctca
    19381 accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta tcgaaacaag
    19441 aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt gctgaaaaaa
    19501 tgttcatcag caacaaaact gtcagcactt ataaaagtcg cttgatggaa aaattagaat
    19561 gcaaatcact gatggatctt tacacattcg cacaacgtaa caaaatcggc taaccacatg
    19621 aagtttttac cctatatttt tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca
    19681 gacggagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcac acttgatcca
    19741 ctacgtctga gcaacaagga attacgttgg ctcgcgagca aaaaaaatct tgtgattgca
    19801 gtacacaagt cccaaacagc tacgttgttg cataccgatt cgcagcaacg gattcgtggt
    19861 attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt aacactccgg
    19921 gaatacgcag atcatcaaaa agcaatggac gcgctggaag acggtgaagt tgatatagtg
    19981 ttatcacatt tagttgcttc gccgcctctt aatgatgaca ttgctgcaac caatcctctg
    20041 ataattacct ttccggcgct ggtcactacc cttcacgatt caatgcgacc gcttacctca
    20101 tcaaaaccag taaatattgc tcgagtagca aattatcccc cagacgaggt aattcatcaa
    20161 tcatttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt agcatccgtc
    20221 tcagccggac agaatgatta ctttattggt agtaacatca ttaccagcag tatgatttcc
    20281 cgttatttta ctcactcctt aaatgtagtg aagtattata actcaccgcg tcaatataac
    20341 tttttattaa ccagaaaaga ttctatcgtt cttaatgaag tactcaatcg atttgttgat
    20401 gctttaacaa atgaagttcg ctatgaagta tcacaaaatt ggcttgatac aggaaatctg
    20461 gcctttctga acaaaccatt agaactcact gaacatgaaa aacagtggat taagcagcat
    20521 cccgatttaa aggtgctaga aaatccttac tcgccaccct attctatgac agatgaaact
    20581 ggctcagttc ggggcgtgat gggtgatatt cttaatatta ttaccttgca aacaggttta
    20641 aatttttctc cgatcaccgt ttcacacaat attcatgctg ggacacagct taaccccggt
    20701 ggatgggata tattgcccgc cgctatttat agtgaagatc gagaaaataa tgtttcattt
    20761 gctgaagcct tcataacaac gccttacgtt tttgtcatgc aaaaagcgcc tgacagtgaa
    20821 caaacattaa aaaaaggaat gaaagttgcc attccctatt attatgagct tcattcgcaa
    20881 ttaaaagaga tgtatccgga ggtagagtgg ataaaagtcg ataacgccag cgctgcattt
    20941 cacaaggtta aagaaggtga acttgatgct ctggtcgcga cacagttaaa ttcgcgttac
    21001 atgatcgacc attactatcc taatgaactt tatcattttc ttattcccgg cgttcagaat
    21061 gcatcacttt cgttcgcttt tcctcgcgga gaaccggaac ttaaggatat tattaataaa
    21121 gcactgaatg caattccccc aagcgaagtt ctgcgcctga ccgaaaaatg gattaaaatg
    21181 cccaatgtga ccattgacac atgggacctc tatagcgagc aattttatat tgttacgaca
    21241 ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt acgctcagtt
    21301 cgtcgtcgta aagttattca gggtgattta gaaaaccaaa tatcattccg gaaagcgctc
    21361 tcggattcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt cattagtcac
    21421 aatagtgcat ttgaacatta tttcactgct gattactata aaaatgcaat gttaccatta
    21481 gaaaacagtg aatccccctt taaagatgtt ttttctaata cgcatgaagt cacagcagaa
    21541 acgaaagaaa accgaacaat atacacacag gtatttgaaa ttgataatgg catcgagaaa
    21601 agatgcatta atcactggca taccttatgt aatctgccag caagcgaaca tgctgtttat
    21661 atttgtggtt ggcaagatat taccgagacg cgtgatttaa ttcatgcact cgaagtagag
    21721 agaaataaag cgatcaatgc aactgtcgca aaaagccagt ttctggcaac aatgagtcac
    21781 gaaataagaa caccaataag ctccattatg ggcttcctgg aacttctgtc gggttctggt
    21841 cttagcaagg aacaacgggt ggaggcgatt tcacttgcct acgccaccgg acaatcactc
    21901 ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg aatcgggtaa ctatcaactt
    21961 caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc tttcggtgcg
    22021 attgctgcaa gcaaatcgat cgcattaagt tgcagcagta cgcttcctga acgctacctg
    22081 gttaagatcg accctcaggc gtttaagcag gtcttatcta atttactgag taatgctctc
    22141 aaatttacta ccgagggggc agtaaaaatt acgacctccc tgggtcacat tgatgacaac
    22201 cacgctgtta tcaaaatgac gattatggat tctggaagtg gattatcgca ggaagaacaa
    22261 caacaactgt ttaaacgcta tagccaaaca agtgcaggtc gtcagcaaac aggttctggt
    22321 ttaggcttaa tgatctgcaa agaattaatt aaaaacatgc agggcgattt gtcattagaa
    22381 agtcatccag gcataggaac gatatttacg atcacaatcc cggtagaaat tacccaacaa
    22441 gtggcggctg tcgaggcaaa agcagaacaa cctataacac tacctgaaaa gttgagcata
    22501 ttaatcgcgg atgatcatcc gaccaacagg ctattactca aacgccagct aaatctatta
    22561 ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc tacacaaagt cagtatgcaa
    22621 cattatgatc tgcttattac tgacgttaat atgccgaata tggatggttt tgagttgact
    22681 cgcaaactcc gagagcaaaa ttcttcctta cccatctggg ggcttacagc caacgcacag
    22741 gctaacgaac gtgaaaaagg gttaaattgc ggcatgaact tatgtttgtt caaaccgttg
    22801 actctggatg tactgaaaac acatttaagt cagttacacc aggttgcgca tattgcacct
    22861 cagtatcgcc accttgatat cgaggccctg aagaataata cggcgaatga tctacaactg
    22921 atgcaggaga ttctcatgac tttccagcat gaaacgcata aagatctacc cgctgcgttt
    22981 catgcactag aagctggcga taacagaact ttccatcagt gtattcatcg catccacggt
    23041 gcggctaaca tcctgaattt gcaaaagttg attaatatta gccaccagtt agaaataaca
    23101 cccgtttcag atgacagtaa gcctgaaatt cttcagttgc tgaactctgt aaaagaacac
    23161 attgcagagc tggaccagga gattactgtt ttctgtcagc aaaatgacta aatagcggct
    23221 cccacaatgt tcaaatgtgg gagctattta ccagcacatc ttacatttat gatgagaact
    23281 cctggcgaat ttgttcccca tgctggtcga gcgttgccgc tcccggcata acatgcgggt
    23341 ccgcgcagcc gctgattttt atcggattcc ccggcatcat tattcccccg gcttcaatca
    23401 acatatttct cgcctgagtt tgtggcaaat taatggcctc agccacactt aataacggcg
    23461 cgacgggtac accaacttca tgtattctgg ctaaccaaac ttcagctgcc tgcgttttta
    23521 acgtccgctc aatatattgt ttaagaatag cctggttttg tacgcgtaaa atattgctgc
    23581 taaatctggg atcattaacc agttccgtaa gctccagtgc ctggcataac gcagaaaaaa
    23641 gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtatcgaaa acatcaaaag
    23701 gtgccatgta gggatggcga tttcccaggc gttgtggtga cttccctgtc gcaatatatg
    23761 ccatcagacc atgctccaga aaactcagcg tggcatcaaa catcgctata tcgacatgcg
    23821 cccctctttg gctcttttcg cggccataaa gtgcactcac tattccgctg aataaataga
    23881 caccgccgca tagatccgca agagaggtac ccacgcgcac tggcggagca tcagggtatc
    23941 ctgtttccat cattatcccg ctcattgcct gaatgatggt atcgtaggca ggagcatctt
    24001 ttagcggacc ggtatgtccg aaacctgacg atgaagcata tataaggcgc gggttgattt
    24061 cttgtaacct ttcccatgaa aacccgagtt tttccattgt acctgggcga aaattctcag
    24121 ctaatacatc cgcttgttta agcatattta taaatatact tttatcgtga tcattcttta
    24181 aatcaagaac cacactctct ttgccatgat taataaaact gtaatagagt gactgtccat
    24241 ccacataggg accaaatgtg cgggtatcat caccatgacc cggcggctca actttaatta
    24301 cccttgcgcc catattacaa agaagttgag ttccgaaagg cccattaagg acatgcgtca
    24361 tatcgataac taataagcct tcaaacggcc ctttgcattc attatttgtc atttgcttat
    24421 gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat caataacatg
    24481 attaattatg aaaccagtcg actgacataa atccacaaag gagccgtgac gacaaaaccc
    24541 agtacgctca cagccaatga cgctgtacca gtgcgggtgt agacattaaa ccggctggca
    24601 ataatgattc cggagaatgc cggcggtaag gcgcctgcca gtaccatcat ctgcaaatgt
    24661 tcgctgttca aatgacatgc cataccaacg agaagcagcg ccagtggcat cagaatcagc
    24721 ttcaggaagg tgttataagc aatttcggca ctgaactcga atttatgtgc agccagagtc
    24781 aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt aaaggttggg
    24841 tcccatgctg ccggaatttt taccccaacc aacaccagga tcgttgccag aacaggtgcc
    24901 catactactg gctcctttgc cgcagaaatt aatgcgctca gattactatt cttcttacca
    24961 tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat tgcattaaca
    25021 ataatagaaa taattgctac cactaaacct gttgatacgg aatcaccata aataggatcg
    25081 agaactgcaa atcccaggaa tccaatggta ggtgaacctg caattaatgc acatactgct
    25141 gcttctgcat gagtacgttt aaaaaatttg tagcaaccga accaggagaa aaagaaacat
    25201 ccgacaataa ccacaagtga tacaagggtc agacgagtgt ccgcaaaaat catttccctg
    25261 tttgcccggg taatagatac aaatagagct gcaggaagag cgtagtttaa taccagttta
    25321 ttaaatgccc gggcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac
    25381 agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat aatagtattt
    25441 ccgataaggg tcccctcacc atcgcataat gagttggcaa gaggagacga tgtcagtatt
    25501 taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc agttcctgct
    25561 gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc ggtggttaaa
    25621 gcatgacgaa gttcatctgt cgtagtgacg ttatagccaa cgccacgaaa cgcatccatt
    25681 aatttgtcat accttgcatg gtgcagcaga tccgttggtg atggtgcacc agcgccgctg
    25741 agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat cgtcaccggc
    25801 aggttatatc gacaaatcgt ttcaatttcc atcccactga aaccaaaagc actatcacct
    25861 tcaatggcaa caaccggaga accagaggtc acgctagcac cgatggcata gcccataccg
    25921 atgcccatga caccccaggt gccacaatcc agacgacgac gtggtttata catatcaata
    25981 atatttcgtg cattatccag ggtatttgcc ccttcattaa ctaaataaat atcctggttc
    26041 tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt aatttaatgg ttgagtatct
    26101 gtgcttaact tttcatgcat tttttgtgca ttttgctgct tgtggatatt taaaatatcg
    26161 cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt ctgccagcat accttgcata
    26221 ctggatgcaa tatcgccaac gactggcaca gcaatggggc ggttgctgtc aatttcctgc
    26281 ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt accgtgtgcc
    26341 agtaaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc cagggcaaac
    26401 gaacgcgcag ccgccgcaga aagtggatgc gtatcttcga ggatcccttt cgccatagac
    26461 attggcagga atggaatctg ggcactttca ataaattcac gaagctgttc atccgcttgt
    26521 gaatacgccg cgcctttgcc aaggataact aatggtcgtt cagcttttgc taatagcgaa
    26581 attgcgctag cgactgactt cgggcatggc aataatgctg gcgacggatt ttcaactttt
    26641 acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa
    26701 tcaagataaa ctccgccagg gcgacccgat actgagaccc ggatagcgcg tgccaatgca
    26761 atgccaagat cctgcggctg attaacgcgg aatgctgctt tggcatacgg ttttgccgca
    26821 ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc gcggtcgctg
    26881 gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa tgcggtcaaa
    26941 ccattgagga atcctggcgc agaaactgtc aggcagatcc ccggtttttg ggtaagaaaa
    27001 ccgcttgccg cagcggcata gcctgccgac tgctcatgac gaaaaccaat ataacgaatg
    27061 ccttccgcct gggcatggcg cgccatatcc gtcacaggaa tacctacaac accataaata
    27121 gtgtcaatac cattctgttt taatgcttca acgatgatat gcataccatc tgtcatttga
    27181 agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc agaagccccc
    27241 gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca
    27301 gctcctgcaa tacagcagcg gtatgctcac ctaatagcgg cgcagcttta atatccggcg
    27361 taaaggcaga gaatttcatt ggacaaccaa cggtcagata ttttccacgc aacggttgtt
    27421 ccacttcgac aacactacca ctttggcgca aagagggatc aagtgaaatt tctttcatac
    27481 ttaaaaccgg tgcacaagga atatcaaact gagtcaaata ggccaccgct tcatgtttat
    27541 caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg
    27601 catgggctgt actgtatgcc ggatcggtaa tccattctgg tttgccgatg gctttacagg
    27661 tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga tcggtttccc
    27721 agcctttaca tttcaggatc cagccaggct gaccgccacc acccgcatta ccaccgcggg
    27781 gaactgcatc accaaatgtg ccattcggat actgcgggta ttcttccaga taccccaact
    27841 tatcgagacg ctgctggtcg cgtaatttca cgcggcaaag gttcaacacg gcatcctgca
    27901 ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta
    27961 aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa cgctgcagcg cttaccagcg
    28021 gcggaccgtc ccaaaaacct gtagtagatg ccgcgccacc cgctgcctga gcaacgtttt
    28081 cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa
    28141 tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatatga tcaatggccc
    28201 ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc attacctctt
    28261 tgccttccgc tgttttggta tttaattcaa tagaacgttt gttactgtta agcatggtga
    28321 agtaaagcgc atcgatatca ggaatatctc gcagctggtg gcgcgttacg tcaccaacgc
    28381 cgggacgttc aattttaatg acgtcagcgc caaaccaggc cagcatctga gtacaagatg
    28441 ggccagattg cacaccagtg aaatcgagaa ctttaattcc ttgaa
//
LOCUS       DAZGTJ010000068        28394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000068 DAZGTJ010000000
VERSION     DAZGTJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.068"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..856)
                     /gene="espL1"
                     /locus_tag="ACHK8E_003436"
     CDS             complement(<1..856)
                     /gene="espL1"
                     /locus_tag="ACHK8E_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFP0345654.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFACQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTTENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVSKDVVLSYIST
                     LGTFEKNIL"
     gene            1379..3307
                     /gene="thrS"
                     /locus_tag="ACHK8E_003437"
     CDS             1379..3307
                     /gene="thrS"
                     /locus_tag="ACHK8E_003437"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0345655.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3311..3853
                     /gene="infC"
                     /locus_tag="ACHK8E_003438"
     CDS             3311..3853
                     /gene="infC"
                     /locus_tag="ACHK8E_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP0345656.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3950..4147
                     /gene="rpmI"
                     /locus_tag="ACHK8E_003439"
     CDS             3950..4147
                     /gene="rpmI"
                     /locus_tag="ACHK8E_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP0345657.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4201..4557
                     /gene="rplT"
                     /locus_tag="ACHK8E_003440"
     CDS             4201..4557
                     /gene="rplT"
                     /locus_tag="ACHK8E_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP0345658.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4674..4798
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4680..4724
                     /gene="pheM"
                     /locus_tag="ACHK8E_003441"
     CDS             4680..4724
                     /gene="pheM"
                     /locus_tag="ACHK8E_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP0345659.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5008..5991
                     /gene="pheS"
                     /locus_tag="ACHK8E_003442"
     CDS             5008..5991
                     /gene="pheS"
                     /locus_tag="ACHK8E_003442"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP0345660.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6006..8393
                     /gene="pheT"
                     /locus_tag="ACHK8E_003443"
     CDS             6006..8393
                     /gene="pheT"
                     /locus_tag="ACHK8E_003443"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP0345661.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8398..8697
                     /gene="ihfA"
                     /locus_tag="ACHK8E_003444"
     CDS             8398..8697
                     /gene="ihfA"
                     /locus_tag="ACHK8E_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP0345662.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8798..9778
                     /gene="btuC"
                     /locus_tag="ACHK8E_003445"
     CDS             8798..9778
                     /gene="btuC"
                     /locus_tag="ACHK8E_003445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP0345663.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9841..10392
                     /gene="btuE"
                     /locus_tag="ACHK8E_003446"
     CDS             9841..10392
                     /gene="btuE"
                     /locus_tag="ACHK8E_003446"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP0345664.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10392..11141
                     /gene="btuD"
                     /locus_tag="ACHK8E_003447"
     CDS             10392..11141
                     /gene="btuD"
                     /locus_tag="ACHK8E_003447"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP0345665.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11220..11684
                     /gene="nlpC"
                     /locus_tag="ACHK8E_003448"
     CDS             11220..11684
                     /gene="nlpC"
                     /locus_tag="ACHK8E_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0345666.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11931..12644
                     /gene="ydiV"
                     /locus_tag="ACHK8E_003449"
     CDS             11931..12644
                     /gene="ydiV"
                     /locus_tag="ACHK8E_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP0345667.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            12707..14143
                     /gene="selO"
                     /locus_tag="ACHK8E_003450"
     CDS             12707..14143
                     /gene="selO"
                     /locus_tag="ACHK8E_003450"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP0345668.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(14147..14338)
                     /gene="hemP"
                     /locus_tag="ACHK8E_003451"
     CDS             complement(14147..14338)
                     /gene="hemP"
                     /locus_tag="ACHK8E_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP0345669.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(14470..15516)
                     /gene="aroH"
                     /locus_tag="ACHK8E_003452"
     CDS             complement(14470..15516)
                     /gene="aroH"
                     /locus_tag="ACHK8E_003452"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP0345670.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15673..16506)
                     /gene="ppsR"
                     /locus_tag="ACHK8E_003453"
     CDS             complement(15673..16506)
                     /gene="ppsR"
                     /locus_tag="ACHK8E_003453"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP0345671.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16839..19217
                     /gene="ppsA"
                     /locus_tag="ACHK8E_003454"
     CDS             16839..19217
                     /gene="ppsA"
                     /locus_tag="ACHK8E_003454"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP0345672.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(19274..20920)
                     /gene="fadK"
                     /locus_tag="ACHK8E_003455"
     CDS             complement(19274..20920)
                     /gene="fadK"
                     /locus_tag="ACHK8E_003455"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP0345673.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(20977..21270)
                     /gene="ydiT"
                     /locus_tag="ACHK8E_003456"
     CDS             complement(20977..21270)
                     /gene="ydiT"
                     /locus_tag="ACHK8E_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0345674.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(21267..22556)
                     /gene="ydiS"
                     /locus_tag="ACHK8E_003457"
     CDS             complement(21267..22556)
                     /gene="ydiS"
                     /locus_tag="ACHK8E_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0345675.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22611..23549)
                     /gene="ydiR"
                     /locus_tag="ACHK8E_003458"
     CDS             complement(22611..23549)
                     /gene="ydiR"
                     /locus_tag="ACHK8E_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP0345676.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23569..24333)
                     /gene="ydiQ"
                     /locus_tag="ACHK8E_003459"
     CDS             complement(23569..24333)
                     /gene="ydiQ"
                     /locus_tag="ACHK8E_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP0345677.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24649..25560
                     /gene="ydiP"
                     /locus_tag="ACHK8E_003460"
     CDS             24649..25560
                     /gene="ydiP"
                     /locus_tag="ACHK8E_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0345678.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(25603..26754)
                     /gene="ydiO"
                     /locus_tag="ACHK8E_003461"
     CDS             complement(25603..26754)
                     /gene="ydiO"
                     /locus_tag="ACHK8E_003461"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP0345679.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26768..28363)
                     /gene="ydiF"
                     /locus_tag="ACHK8E_003462"
     CDS             complement(26768..28363)
                     /gene="ydiF"
                     /locus_tag="ACHK8E_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0345680.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILETT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT         7023 a         6871 c         7340 g         7160 t
ORIGIN      
        1 aaagaatatt tttttcaaaa gtacccaacg ttgatatata agataacacg acatctttac
       61 ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg
      121 ccatcattcg acagaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc
      181 gactccctgc aggaggatgg cgaagtaatg atctgaactc cgcgtcccgg actaagggaa
      241 catgctgctg tatggattca acggaggagt aatgtttaat cagattgact ttgccttttc
      301 catttctcag tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag
      361 aaggagattg cgaaaaaaat gcactaccat tgagatttac tgttggttgt atagtttttt
      421 ctctatggat ttttttagac agtttcatac aatggttacg attttcggta gttaactgtt
      481 cctgttcaat ataatccagt atgcttcgca aatggttagc tttaataggc gtggtttcca
      541 gtaataagga aatgatttct tttttatagt gtaatgtaat gagtatattt tctgcatgga
      601 tgggtgaagt ggcaaagtta atggtaaaaa gggcagtgga ataacaattt tgtgatattt
      661 ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcattgaa cttaagtcag
      721 aggtatcaag tagggtgatg agttggcacg caaattgatt aataggctct gatgaaggcg
      781 ttggtatgtc gctatcgatg tgcattctat gaggagactt ataatgagtt ctgataataa
      841 tgtcgttttg actcataatc tattcgcctt atatatgtct ggaggttttg aggatgatga
      901 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ctataagaat tgtgtcactt
      961 ttatcattgt gatatgacca cttcgtcatt aatcttatga tcgtttaaat agtaccgcca
     1021 aattacgcaa cgaactttgc tggattcagt ttatgctgct gcaaatccgc tcgcagtaaa
     1081 cctttcagac gctccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac
     1141 tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta
     1201 gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct
     1261 gattgcgaac caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac
     1321 aaatttttct ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat
     1381 gcctgttata actcttcctg atggcagcca acgccattac gaccacgctg taagccccat
     1441 ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa
     1501 tggcgaactg gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac
     1561 cgccaaagac gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca
     1621 cgcgattaaa caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg
     1681 tttttattac gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga
     1741 gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg
     1801 gcacgaagcg cgtgaaactt tcgccaaccg tggtgagagc tacaaagtct ccattcttga
     1861 cgaaaacatc gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat
     1921 gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac
     1981 ggcaggggct tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac
     2041 ggcgtgggca gacaaaaaag cgcttaacgc ttacctgcag cgcctggaag aagccgcgaa
     2101 acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcacc
     2161 gggtatggta ttctggcaca acgatggctg gaccatcttc cgtgaactgg aagtgtttgt
     2221 tcgttctaaa ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg
     2281 tgtcctgtgg gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacgtc
     2341 ttctgagaac cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt
     2401 caaccagggg ctgaagtcgt atcgcgattt gccgctgcgt atggccgagt ttggtagctg
     2461 ccaccgtaac gagccgtcag gttcgctgca tggcctgatg cgtgtacgtg gttttaccca
     2521 ggatgacgcg catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat
     2581 ccgtttagtc tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc
     2641 cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct
     2701 ggcagttgcg ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt
     2761 ctacggtccg aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg
     2821 tacagtacag ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggcgaaga
     2881 caacgagcgt aaggtaccgg taatgattca ccgcgcaatt cttgggtcga tggaacgttt
     2941 catcggtatc ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccggttca
     3001 ggttgttatc atgaatatta ccgattcaca gtctgattac gttaacgaat tgacgcaaaa
     3061 actatcaaat gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa
     3121 aatccgcgag cacactttgc gtcgcgtccc gtatatgctg gtctgtggtg ataaagaggt
     3181 ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt
     3241 aaatgaagtg atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga
     3301 ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg
     3361 cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt
     3421 gagtctgaga gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag
     3481 ccctaacgcc gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa
     3541 gagcaagtct tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa
     3601 attccgtcct ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt
     3661 tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca
     3721 ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt
     3781 ggtcgaatcc ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa
     3841 gaagaaacag taaggccttc aagtagcact gtctgtggag cctttgggct tcacaggcat
     3901 tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat
     3961 taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca
     4021 taagcacgct aacctgcgtc acattctgac caaaaaagcg accaaacgta aacgtcacct
     4081 gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta
     4141 cgcataagcc gttaacgttt tttaactttt taattagaat atagatacag gagagcacat
     4201 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     4261 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     4321 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     4381 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     4441 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     4501 ttcgacaaag tagcgttcac cgctctggtt gaaaaagcga aagcagctct ggcataagcc
     4561 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     4621 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     4681 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgactccag gaggctagcg
     4741 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     4801 gcgcgttttg aaaaataaaa agcctgtctc atcaggcgta attgtcgcag ccagtctgga
     4861 cacggccagc gcggagaaac cggagcgtac ttaagtacgt gaggatttcg agcactgccc
     4921 gggaccaaaa tggcaagtaa aatagcctga tgggatgggc tctaagtcca acgaaccagt
     4981 gtcaccactg acacaatgag gaaaaacatg tcacatctcg cagaactggt tgccagtgcg
     5041 aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg cgtcgaatat
     5101 ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct gccgccagaa
     5161 gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca gcaggcgctg
     5221 aatgcgcgta aagcggaact ggaaagcgct gcactgaatg cgcgtctggc ggcggaaacg
     5281 attgatgtct ccctgccggg tcgtcgcatt gaaaacggcg ggctgcatcc ggtgacccgt
     5341 accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc aaccgggccg
     5401 gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca ccacccggcg
     5461 cgcgctgacc acgacacttt ctggtttgac gctacccgcc tgctgcgtac ccagacctct
     5521 ggcgtacaga tccgcaccat gaaagctcag cagccaccga ttcgtatcat cgcgcctggc
     5581 cgcgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca gatggaaggt
     5641 ctgattgttg ataccaacat cagctttacc aacctgaaag gcacgctgca cgacttcctg
     5701 cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt cccgtttacc
     5761 gaaccttctg cagaagtgga cgtcatgggt aaaaacggta aatggctgga agtactgggc
     5821 tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca tcgacccgga agtttactct
     5881 ggtttcgcct tcgggatggg gatggagcgt ctgaccatgt tgcgttacgg cgtcaccgac
     5941 ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata aggcaggaat
     6001 agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg attgatagcg
     6061 atgcgctggc aaatcaaatc actatggcgg gcctggaagt tgacggtgta gaaccggttg
     6121 ccggtagctt ccacggcgtg gtcgttggtg aagtgattga gtgtgcgcag catccgaacg
     6181 ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga tcgcctgctg gacatcgtct
     6241 gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agcgaccatt ggtgctgttc
     6301 tgccgggcga tttcaaaatt aaagcggcga aactgcgtgg cgaaccgtct gaagggatgc
     6361 tgtgctcctt ttctgagctg ggaatttctg acgatcacag cggcattatc gagctgcctg
     6421 cagatgcgcc gattggcacc gacatccgcg aatacctgaa actcgatgac aacaccatcg
     6481 aaatcagcgt aacgccaaac cgtgccgatt gcttaggcat tatcggcgtg gcgcgtgatg
     6541 ttgccgtgct gaaccagctg ccgctggttg aaccggaaat cgttccggtt ggtgcggcca
     6601 tcgacgacac gctgccgatt acagtcgaag cgccggaagc ctgcccgcgc tatcttggcc
     6661 gtgtggtaaa aggcattaac gttaaagcgc caactccgct gtggatgaaa gaaaaactgc
     6721 gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat gtgctgctcg
     6781 aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc attgtggtgc
     6841 ggatggcgaa agagggcgaa actctggtgc tgcttgatgg cactgaagcg aagctgaatg
     6901 ctgacaccct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc atctttggtg
     6961 gcgaacactc tggcgtgaat gacgaaacgc aaaacgtcct gctggaatgc gctttcttca
     7021 gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcatactgat gcgtctcacc
     7081 gttatgagcg tggcgttgat ccggcactgc agcacaaagc gatggaacgt gcgacccgtc
     7141 tgctgatcga catctgcggt ggtgaggctg gtccggtaat tgatatcacc aacgaagcaa
     7201 cgctgccgaa gcgtgccacc attactttac gtcgcagcaa actggatcgc ctgatcggcc
     7261 atcatattgc tgatgagcag gtaactgaca ttctgcgtcg tctcggctgc gaagtgaccg
     7321 aaggcaaaga cgaatggcag gccgttgcgc cgagctggcg tttcgacatg gagattgaag
     7381 aagatctggt cgaagaagtc gcgcgtgttt acggctacaa caacatcccg gatgagccgg
     7441 tacaggcaag cctgattatg ggtactcacc gcgaagccga tctgtcgctc aagcgcgtga
     7501 aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc gttgatccga
     7561 aagtgcagca gatgatccat ccaggcgttg aagccttact gctgccaagc cctatttccg
     7621 ttgaaatgtc agcaatgcgt ctttccctgt ggaccggcct gctggcaacc gtggtgtaca
     7681 accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag tggtctgcgt ttcgtaccag
     7741 atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg atttgcggta
     7801 accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat gatttgaaag
     7861 gcgatcttga atccgttctc gacctgaccg gtaaactgaa tgaggttgag ttccgtgcag
     7921 aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa ggtgaacgta
     7981 ttggttttgt tggggttgtt catcctgaac tggaacgtaa actggatctt aacggtcgca
     8041 ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct caggcgcgcg
     8101 agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca gaaaacgttc
     8161 ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta gttggcgtaa
     8221 acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc ctcgccataa
     8281 gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc gctaccgtcg
     8341 ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat tgaacctatg
     8401 gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct tagcaagcgg
     8461 gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct ggaaaacggc
     8521 gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa tcaacgcccg
     8581 ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt ggtgaccttc
     8641 agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga cgagtaatct
     8701 gatctaacta aaaaggccgc tctgcggcct tttttctttt cactgtcgaa gagtcaccgt
     8761 aaaatcaacg ccatgacact tcagcagaac ggataccatg ctgacacttg cccgccaaca
     8821 acagcgacaa aatattcgct ggttattatg cctgtcagtt ttgatgctgc tggcgcttct
     8881 cttaagcctt tgcgccggtg aactatggat ctcgccaggt gactggtttt ctcctcgtgg
     8941 cgaactgttc gtctggcaaa ttcgcctgcc acgtacgctg gctgtattgc tggttggtgc
     9001 ggcgctggct atatccggcg ctgtaatgca ggcgttgttt gaaaatcctc tggcagaacc
     9061 tggactactt ggcgtctcta acggcgcagg cgtggggctt atcgccgcgg tattgcttgg
     9121 gcaagggcaa ctccccaact gggcgctagg gctgtgtgcg attgctggcg cgcttatcat
     9181 cactttaata ctcttacgtt tcgctcgtcg tcatctttcg accagtcggt tattgctggc
     9241 tggcgttgca ttagggatta tctgtagcgc actaatgacg tgggctatct acttttccac
     9301 ctctgttgat ttacgtcagc tgatgtactg gatgatgggc ggttttggcg gcgtagactg
     9361 gcggcaaagc tggctgatgc tggcattgat ccccgtgttg ttgtggatct gttgtcagtc
     9421 caggccgatg aatatgttag cacttggcga gatctcggcg cggcaactgg gtttacccct
     9481 gtggttctgg cgcaatgtgt tggtcgcagc gaccggctgg atggttggcg tcagtgtggc
     9541 gctggcgggt gctatcggct ttattggtct ggtgatccca catattctcc ggctgtgtgg
     9601 tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg gcaggggcga gcgcattgct
     9661 gctggccgat attgtagcgc gcctggcatt agctgccgca gagctgccta ttggcgtggt
     9721 caccgcaacg ttgggtgcgc cggtgtttat ctggttattg ttaaaagcag gacgttagcc
     9781 gcaaaaagac ggtctatgat taaaagctaa gatttttact gaccacaccc aggagaaacg
     9841 atgcaagatt ccattctgac gaccgtagtg aaagatatcg acggtgaagt gaccacgctg
     9901 gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg cctcaaagtg tggcttaacg
     9961 ccgcaatatg agcagttgga gaatattcag aaagcctggg ccgatcgtgg ttttgtggtg
    10021 ctgggattcc cgtgcaacca gtttctggaa caagaaccgg gcagcgatga agagattaaa
    10081 acttactgta ccaccacatg gggggtgacg ttcccgatgt tcagtaagat tgaagttaat
    10141 ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg cagcgccgac cgcagtcgcg
    10201 ccggaagaga gcggattcta tgctcgtatg gtcagcaaag gtcgtgcacc gctgtaccct
    10261 gatgatattt tatggaattt tgagaaattt ttagttggca gggacggatt ggtcatccag
    10321 cgtttttccc cggatatgac gccggaagat cccattgtga tggaaagcat taaactggcg
    10381 ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt tgcggaatct acccgcctgg
    10441 ggccgctttc tggcgaggtt cgggctgggg agatcctgca cctggtgggg ccgaatggcg
    10501 cgggtaagag taccttactg gcgcgaatgg ccggaatgac cagcggtaag ggaagcattc
    10561 agttcgcggg gcaaccactg gaagcatggt ccgcaacaaa actcgcgcta catcgcgcct
    10621 atctttcaca acaccagacg ccaccgtttg caatgccggt ctggcactac ctgacactgc
    10681 atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg ctggctcttg
    10741 atgacaaact cggacggagc accaatcaac tttccggcgg tgaatggcaa cgcgtacgtc
    10801 ttgctgcggt ggtgttgcaa atcacaccac aagccaatcc cgcaggccaa ttgctgtttc
    10861 ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac aaaattctga
    10921 gcgcactgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc aaccacacct
    10981 tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc agtggacgca
    11041 gggaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc
    11101 tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattaa tatcttgaaa
    11161 atcgggtaat tacgacgcta aattaatgca agaataaaaa aacagaggaa ccgtacggaa
    11221 tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc caccataaag
    11281 caccgccgcc aaatgccaga ctttctgatt cgattaccgt tattgccggt ttgaacgacc
    11341 agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg
    11401 actgttcggg gtttgtggtt gtgacgatgc gcgatcgttt cgatttgcag ctgccccgtg
    11461 aaaccaaaca acaagcctct atcggcacgc aaattgataa agacgagttg ctgcctggtg
    11521 acctggtctt tttcaaaacg ggctccggac aaaatggttt gcatgtaggt atttatgata
    11581 ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc tcacttgata
    11641 atgtctattg gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc gaaaaatgga
    11701 cggcgatgat acatcgccgt ccatttactc ctgaatacag tagtattttg cagacaaaaa
    11761 ttccgtaaat ggattattcg tttataatca ggataatcta aaaaaaggac ccctgaaaaa
    11821 gtggttaaca tggaacaaat gaaagttact ccttctgtaa ttgttatgtc ttatggcctc
    11881 atccctttta gccggatact gaaaaacatc cttcgagagg gacggttacc atgaagattt
    11941 ttttggagaa tctttatcat tctgattgct atttcctgcc gataagagat aaccagcaag
    12001 accttgtcgg cgttgagtta attacccatt tctccagtga agatggtacg gttcgtatcc
    12061 cgacgagtcg ggttatagcg caacttaccg aggagcaaca ttggcaatta ttttcagagc
    12121 agttggaatt actaaaatca tgccaacact tttttattca acataaactc tttgcctggc
    12181 ttaatttaac gccacaagtg gctacattat tattagacag ggataattac gcaggcgaac
    12241 tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat ctcgatgaag
    12301 gaaaagataa tcgcgatctt ttgtccttat cgcaaatgta tccccttgtg ttaggtaatt
    12361 tgggcgcagg caatagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtcatgc
    12421 tggataaaag ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg
    12481 cgatccaggc tcaaatttcc ccatgttgca attgcatcat cgcggggggc attgatacgc
    12541 cagaaataat ggcacagatt accccctttg atttccatgc tctccaggga tgcctgtggc
    12601 ctgccgttcc tgtaaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg
    12661 tgtttaagac gagagtaacc gtctacacta tcaaatagga ggatctatga ccctgtcttt
    12721 tgttacccgc tggcgcgatg aattgccaga aacctataca gcactttccc ctacgccttt
    12781 aaataatgcc cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc
    12841 gtcgctgttt aaaaatggcg caggtgtctg gggcggcgaa accctactgc ctggtatgtc
    12901 accactggcg caggtttaca gtggtcatca gttcggcgtc tgggcgggcc aactgggtga
    12961 tgggcgcggc attttactcg gcgaacaacg gcttgctgat ggcactacaa tggactggca
    13021 tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg cggttttacg
    13081 ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac
    13141 ccgcgcgtta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg
    13201 cgcgatgctg atgcgtgtgg cactaagtca tctgcgcttt ggtcatttcg aacattttta
    13261 ctatcgccgc gagccggaaa aggttcgtca gttggctgac tttgccattc gtcattactg
    13321 gtctcatctt gaagatgatg aggacaaata ccgtctctgg tttagcgatg ttgtcgcacg
    13381 taccgcatcg ttaattgccc aatggcagac ggtcggcttt gctcatgggg tgatgaatac
    13441 cgacaacatg tcgctgctgg ggctgacgct tgattacggg ccgtttggtt ttcttgatga
    13501 ttacgaaccc ggttttattt gtaatcactc ggatcaccaa gggcgttaca gctttgataa
    13561 tcaacctgcc gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt cgccatttgt
    13621 cgccgttgat gccctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta
    13681 tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg ataacgcgct
    13741 actaaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacatttcg
    13801 catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg agtttattga
    13861 tcgtgcggca tttgatgact ggtttgcccg ttatcggagg cgtttgcaac aagacgaggt
    13921 tagcgatatt gagcgtcagc aactgatgca aagcgttaac ccagctctgg tgttgcgcaa
    13981 ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgacgg aattgcaccg
    14041 cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactacg ccagtcgtcc
    14101 acctgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggttac ttggtcaaca
    14161 gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca
    14221 gtttgccatc cggacctaac agtgtctggc tggtaattcg ccggatgggc tgcggggaag
    14281 cggtcttgtg attggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa
    14341 aaattaataa taatttatat aacaatgatt cttattatca atattatggc gggccagagc
    14401 aagcagtaat ttgttgagaa gacaacaagt tgcgcagaaa tgcccatccc gtcaggaatg
    14461 ggcacgcatt cagaagcgag tatctaccgc agaggcgagt ttttcgacca ggcgttcggt
    14521 atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtgagcg gctgaccgcc
    14581 gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt
    14641 agatccattg cggatttgct gacaaatatc ctcacaaact tctaactggc gtcggtgctg
    14701 cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg
    14761 cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg
    14821 cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct gaccattttt
    14881 gtctggcgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cggcaatccg
    14941 cgtgttgcca tcggtaccat ttttaaaacc taccggacag gagagtgccg aagccatttc
    15001 acggtggatc tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc
    15061 aataaactga ccggtcacca tatcgaggaa ctcagtcgcg gtagggacgc ccagctcatt
    15121 tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat agctgccgtt
    15181 taaatctgga tcggagatta gccctttcca gccgacaaca gttcgtggtt tttcaaaata
    15241 ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt
    15301 ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag
    15361 tcgcttatct tcaccattca gtattttttc aattctgcgg cgggagtcgg tgacatgggt
    15421 ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc
    15481 aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct
    15541 tctacggcag gactaccggg aagtgatgcg catcacgata aaccaatcgc agctgatttc
    15601 aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aacaagctaa
    15661 tgcactagtt ctctagtaca ttcgacggct aaggcccatg atatcgagga tcttggtggc
    15721 aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa
    15781 cgcttccact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc
    15841 ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt
    15901 atgctgaagc ggtttgagcg acgcgggtag cacaagatta tccatatcgt cggcaataaa
    15961 ggggtagttt gccgcgcgga taccaaactg cattgccaga tacagactgg tgggggtttt
    16021 accacagcga gaaacgccga gcaggatcac ctgagcctgg tccagattgc gtaacgaaat
    16081 gccatcatcg tgggcgaggg tgtaatcaat cgcagcaatg cgcgcatcat atttattgag
    16141 attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc
    16201 ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaagccct cactttgcaa
    16261 gatgatggcg cgaatctccg gcaacacgat ggagtagaag accagcgggc gcacacctgt
    16321 ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac
    16381 aaacggcagc gtaatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa
    16441 tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt
    16501 atccattttg ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc
    16561 acagaagcgt agaacgttat gtctggttta taaaatgaac cttcaatttt attttttatg
    16621 aaaacagcat ttcattttta cggtttcgtt tataccgatg gtttatgtgg aaattgtcga
    16681 agagagcaga tttgcgcaac gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt
    16741 gaacgattca ccgttttttt catccggtta aatatgcaaa gataaatgcg cagaaatgtg
    16801 tttctcaaac cgttcattta tcacaaaagg attgttcgat gtccaacaat ggctcgtcac
    16861 cgctggtgct ttggtataac caactcggca tgaatgatgt agacagggtt gggggcaaaa
    16921 atgcctccct gggtgaaatg attactaacc tttccggaat gggtgtttcc gttccgaatg
    16981 gtttcgccac aaccgccgac gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc
    17041 gcatttatga actgctggat aaaacggata ttgacgatgt tacccagctt gcgaaagcgg
    17101 gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca gcctgagctg gaaaacgcca
    17161 tccgcgaagc ctatgcacag ctttccgccg atgacgaaaa cgcctcgttt gcggtgcgtt
    17221 cctccgctac tgcagaagat atgccggacg cttcttttgc cggtcagcag gaaactttcc
    17281 tcaacgttca gggttttgac gccgttctcg tggctgtgaa acatgtattt gcttctctgt
    17341 ttaacgatcg cgccatctct tatcgtgtgc accagggtta cgatcaccgt ggcgtggcgc
    17401 tgtccgccgg tgttcagcgg atggtgcgct ccgacctcgc atcttctggc gtgatgttct
    17461 ccattgatac cgaatccggc tttgaccagg tggtgtttat cacttccgca tggggccttg
    17521 gtgaaatggt cgtgcagggt gcggttaacc cggatgagtt ttacgtgcac aaaccgacac
    17581 tggcggcgaa tcgcccggct atcgtgcgcc gcaccatggg gtcgaaaaaa atccgcatgg
    17641 tttacgcgcc gacccaggag cacggcaagc aggttaaaat cgaggacgta ccgcaagaac
    17701 agcgtgacat cttctcgctg accaacgaag aagtgcagga actggcaaaa caggccgtac
    17761 aaattgagaa acactacggt cgcccaatgg atattgagtg ggcgaaagat ggccacaccg
    17821 gtaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg
    17881 agcgttatac gctgcattca cagggtaaga ttatcgccga aggccgtgct atcggtcatc
    17941 gcatcggtgc gggtccggtg aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac
    18001 ctggagacgt gctggtcact gacatgaccg acccggactg ggaaccgatc atgaagaaag
    18061 catctgccat cgtcaccaac cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg
    18121 aactcggcat tccggcggta gtgggctgtg gtgatgcaac agaacggatg aaagacggtg
    18181 agaacgtcac tgtttcttgt gccgaaggtg ataccggtta cgtctatgcg gagttgctgg
    18241 aatttagcgt gaaaagctcc agcgtagaaa cgatgcctga tctgccgttg aaggtgatga
    18301 tgaacgtcgg taacccggac cgtgctttcg acttcgcctg cctgccgaac gaaggcgtcg
    18361 gccttgcgcg tctggaattt atcatcaacc gtatgattgg cgtccaccca cgcgcactgc
    18421 ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt
    18481 ttgattctcc gcgtgaattt tacgttgggc gtctgactga agggatcgcg acgctgggtg
    18541 ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg aacgaatatg
    18601 ccaacctggt cggtggtgag cgttacgagc cagatgaaga gaacccgatg ctcggcttcc
    18661 gtggcgcggg ccgctatgtt tccgacagct tccgcgactg cttcgcgctg gagtgtgaag
    18721 cagtgaaacg tgtgcgcaac gacatggggc tgaccaacgt tgagatcatg atcccgttcg
    18781 tgcgtaccgt agatcaggcg aaagcggtgg ttgaggaact ggcgcgtcag gggctgaaac
    18841 gtggtgagaa cgggctgaaa atcatcatga tgtgtgaaat tccgtccaac gccttgctgg
    18901 cagagcagtt cctcgaatat ttcgacggct tctcaattgg ctcaaacgac atgacacagc
    18961 tggcgctcgg tctggaccgt gactccggag tggtgtctga actgttcgat gagcgcaacg
    19021 atgcggtgaa agcactgctg tcgatggcga ttcgtgccgc gaagaaacag ggcaaatatg
    19081 tcgggatttg cggtcagggt ccgtccgacc acgaagactt tgccgcatgg ttgatggaag
    19141 aggggatcga tagcctgtct ctgaacccgg ataccgtggt gcaaacctgg ttaagcctgg
    19201 ctgaactgaa gaaataaaat aaatccccgg cggcgttttg tcgccgggtt tatgtgatcc
    19261 ccgaagatga aacttattca atctcttcac agacatcctg agttaatcgc cgcataatat
    19321 ctttccttaa caaaaacttt tgtattttac ctgaggctgt gcgcggtagt ttttcgatta
    19381 ccacgatatg ttcaggatat ttatatttcg cgacccgttt acggctaaaa aatgccacta
    19441 catcttccag cgataatgaa tgatgcggtg ctttcagcac gacataagcg catgaacgtt
    19501 cacctaagcg ttcatcgggc attgcaacca cacaagcatc gtgaatttta ggatgctgca
    19561 ataaaatatc ttctacttca cggctgctaa tattttcgcc gccgcggaca ataatatctt
    19621 tcttgcgccc ggttattttt atatagccag cctcatccat gcggcagaga tcgccgctgt
    19681 aataccagcc ttcttcatcc agcgcacggg cggttaattc aggttcatca aaatacccca
    19741 taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt aaagtcttgc
    19801 gtgcgtcatc gaccacttta atctctacac ctgcggcagc gtaaccatcg gtgtgtataa
    19861 agcgtgacaa aggatcatcg agattcacca ccgcatgcgg cgaactttct gtggaaccat
    19921 aaacacttaa taatttaatg ccgcgctgct ggcattcacg cgccactttt ttggggattg
    19981 tggtaccgcc gcaaagaaag aaacgcagcg ctgaaaggtc tgcgggctgt ttctccagta
    20041 aattcaaaag atcatagaca aacggcgttg cgccgagcat acaggtgcaa cgctgctgct
    20101 caagcagcgc gagacacgca gcaggagtga aaatatctaa caacacgctg cgagcaccaa
    20161 tcaaaaaagg tgctgttacg ccatgcagaa agcccgttgc gtgaccaagt ggcgcaggca
    20221 tcataaagac atcctgccag gtcagattta gccgcgcgca ataagcccgc tcactggcga
    20281 gaatattgtt atgcgttagc atcacgccct ttggcagacc ctcggttccg gaggtaaaca
    20341 gcaccgcggc taattcatcg ccgtggaccg ttatcgccgt ggtcagagga gtattgtcgg
    20401 cgataatctg acttaatgag agagcagagg tggcgggagc cagtttgtcc acgccgacaa
    20461 tttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc ggacgcgttt
    20521 gtttaaacaa cgtcggtgca aagaacattt ttgcctgaca cttattgagc acccacacca
    20581 gttctgcttc tcgccaggaa ggcaacagcg gcacggaaac cgcaccggtt ttcaggcagg
    20641 caagatagat aacggtaaat tcacaccagc ctggcagttg aaatgcgatg cgatcgcctg
    20701 attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga tcgagcgcgc
    20761 tataagtgta cgttgcacca tgattgtcga ccacggcaat tttgtctggc atcgcacgag
    20821 cggtctgctg ccagtaatcg gccagtgaag catcgcccca taacccttgc tgacgatacg
    20881 ccgcacgacg ttgttcgttg aacgttaatg tcactttcat tttggactct cgaaacagaa
    20941 catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa attcaatacc
    21001 gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttgccgcaca gcacccgaca
    21061 ggtgccgcac tccagacaac cggcggaatc gaaatgaatg tttcctgcgt catcctgctt
    21121 ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat cgggatttgc
    21181 cgccagaatg atatgcgggt ggccctcatc aacatggaat ttatttacgc ctaatttgat
    21241 gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat gccatctttc
    21301 agcaagttga tcagtccaat tttcttcgcg tgtcccatga tcattttgcg taccggttgg
    21361 ttaggtttgc cgtcgatggt gaacatctcg ttcatgatgt cggcgaccat tcgtgggtat
    21421 tggctaaaca ggcgcgggtt ttccatcagc gccgggatct tgcgaaaatg ctgcatatca
    21481 cgcatgacgc agctttgttc cagctcacgt ttgtattgcg caagactgct ggcggagaaa
    21541 tccgcgcgtt ctttggcggc gatcactgtt gtggcggcag cctgagccga tgcaatggct
    21601 aaatccatgc cgcggaccgt aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg
    21661 atcatcacgc catcgttaac cagctgcggc accattgcca gaccgccctc cggcaccata
    21721 tgcgcggagt attcaagcag tttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt
    21781 ttaaaatctt ccagcatttg cggcacactt ttttgcgcat gggcgatatc acccaatcca
    21841 caaaccagcc ccaacgatac ggaatctttg ttggtataga ggaatccgcc gcccatcagg
    21901 ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt aacattaaag
    21961 cgatcgttga tctgttctgg tgtcaggcct ataacctctt taacaccaac agcgtaatga
    22021 tgcggatcgg aagcggaaac cattcccagc gagcggccaa gcatcgagtt aacgccatca
    22081 gccagaatca ccacattcgc ttcgagaata tcatccccgg cctggacgcc agtgaccttg
    22141 tttccttcac gaaccagtgc atcgacgcgt acgcccggga taaactgcgc gcccgcctgc
    22201 tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata agacgcgtgt
    22261 tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa cggcgctttc ttcggttaag
    22321 aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg ctgatgctgc aaagcctgga
    22381 ataattgcgt caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt acatccggca
    22441 ctgtcgccgc gttctatcac caggacatcc agcccggctc gcgccatgac cagtgcggca
    22501 acgctaccgg caacgcccgc accgaccaca atggcatcaa atttgtcatc cgacatgcgt
    22561 tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggaa tcagcgactc
    22621 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
    22681 tagttgaaga ttggcgcatt tttatcttta ttaatggcga caatcacttt tgcgccgttg
    22741 ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag atcggatttc
    22801 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
    22861 attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg gaccattttc
    22921 aggtcatcct gcgctgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
    22981 tccacgctgc ttgccgcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggggca
    23041 acataaggta ccgtttctgt cggacactga ttagaggtat cactggtgca cggttcctga
    23101 acaccgggcg caagggtaat gatcgccagc gggctattga tcttttcctg tgcgaacgct
    23161 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgcagtg
    23221 gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgcagccag agctttaccg
    23281 cgtttcgtcg cggccagcag caacatagaa gggtgtttat ctttcagcag ggcagcaatg
    23341 ctttcggcgt aattttcagt acgttgcagc gcgtcgtttt gcgcaagaac ataaatgcat
    23401 tttggcccat aaggcataac tgtctgcgcc tggtcggtat tttgtacaat ggcatacacc
    23461 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
    23521 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
    23581 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgagaatgat
    23641 gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc agttcagcaa gtggtgcgct
    23701 ctggctccag ccaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
    23761 gatggctttc atcgaaggaa tacgtggtac gttaatatcg gaggtgacgc agagcacggc
    23821 tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt
    23881 attgccctga cgctgaatgg cactcactgc attaatcacc ggaagttgca gaatttctcc
    23941 gaccagcaag ccaacctgct gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
    24001 atcgaagccg atcttttcaa ctgctgtcgc cagcgccttt gcggtatcga gaggcagtgc
    24061 atgctcaagt tgtgcatcct gcaccatata caggctgtgc ggcccgcggg atagcacgtc
    24121 tttgcgcact ttcgagttct gcaacaaaga gccgccgacg gtcagcgcgg ctatctcatc
    24181 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
    24241 ggcgtcggca ttgtcgaaat tcagggtata ttctggagtg acaacaatgt cctgttcttc
    24301 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
    24361 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
    24421 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
    24481 caaaagtgca atttgcggca atgttgttat tccatgaccg tacaatcttg cattcagtgt
    24541 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
    24601 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
    24661 tgttttgata acgccagcga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
    24721 tttgcattta gcgatgatcc aaaatgggag tccggacatc acgttcatga caatgaaacc
    24781 gagttgattt acgtcaagaa aggggtagca aggtttacca tcgattcttc gttatatgtc
    24841 gcgcatgccg atgacattgt ggtgatagaa cgtggcaggt tgcatgcggt ggcctctgac
    24901 gttaacgatc cagcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
    24961 gaggaaaatc agctactgca accgcattct tgcccggtaa ttgccgcagg gcagggaaaa
    25021 gaagtcatta aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
    25081 caaacatctt cgttatggga cgcatttgcc tatacgttag caattcttta ctatgaaaac
    25141 tttaaaaatg cttatcgttc ggagcagggg tatattaaaa aagatgttct gataaaagat
    25201 attcttttct atctgaataa taattatcgc gaaaaaatca ctttagagca gttatcgaaa
    25261 aagtttcgtg ccagcgtcag ttatatttgc catgaattta ccaaagagta tcgcatttcc
    25321 cctattaact atgttattca acggcgtatg acggaagcga aatggtcact cactaatact
    25381 gaattatcac aggcagagat ctcctggcgt gtgggttatg aaaatgtcga tcactttgcc
    25441 aaactgtttt tgcgccatgt cggctgttcg cccagcgatt accgcaggca atttaaaaac
    25501 tgttttgcgg aacaagaaat cctatctgaa tttcctcaac cagtaagtct tgccggataa
    25561 atctggcggc gcagtttgtt aagaagctgc gccgcctgca gattatttgt tctgataatc
    25621 tttcaggatc tgccgacctg ctacgtaaat cataatttcg tctgtaccgc cgccgatacg
    25681 ttcacaacgg acatcacgcc agaagcggga gacgcgcgcc tcatcggtat agcccagacc
    25741 gcccatgatt tgaatcgcat catcaatgac ttccattgcg gtacgtgcgc agtacagttt
    25801 tgccagcgcc gcgctggtgc gcagtgactg atgctgatcg gcttgccatg ccactttcaa
    25861 caccatgttg cgcatgttgt cgatcttaat cgccatcagc gccagttttt cctggatcat
    25921 ctggttatga ccaatgggct taccaaaagc gatacgctgg ttggcatagc gggcggcatc
    25981 ttcgaatgcg cattcggcaa agccggtgct gcgcgcggcg ttgatcaggc gctccatctc
    26041 aaagttgtac atcacattga ggaaacccat tccttcttcg cccaccatgt cgctctcttc
    26101 aacttccacg ttgtcgagat agacttcgca ggtgctgagc atatgccagc cgattttatg
    26161 cagtggattg attttaatgc cgggcttact ggagtcgacc caccacagag taaaggcctt
    26221 tttcggatct ttcggttgcg gatcgcgcgc caacaccagc atatatggat actctttcgc
    26281 accggtaata aaggttttct gtccgttgat gtaaaccttg ccgtttttac gcgtgtaagt
    26341 ggtagtggca ctgttgttgt ctgagcctgc gcctggctct gtcaatgcca gggcataggc
    26401 gggatcaccc gtttccaggg tgctttccgc cgttttacgt agctgctctg cagaaccgaa
    26461 acggcgcata ctgtgaatac attgaccgtt ggtgatcaaa aatgccgggg caccgcattt
    26521 tgacacttcc atcagcgcca gcatttgggt gacgtaatcc gcagggatac caccaaattc
    26581 ttccggcacg ccaagcatgg aaataccgtt atccgccagt gcccgcataa attcacgcgg
    26641 gtatgtcccg ttttgatcgc aggtgcggaa atactcttcc ggaaagttag tcgtaatcag
    26701 ttcgcgaata ctggccagca gcagttcttg ttcttcagtt aaagaaaaat ccatcattat
    26761 actcctgtta atgagccgct tcaggcagga caaaacccat cgccgcatcg ataaataatc
    26821 tttcgtccat cagtttgagt tctggcgaaa tcactggggt gaaatccatt ttgtcgagaa
    26881 tatctttttg taaatcgacg ccaggagcga tttcgattaa atgcaggccg tcttctttca
    26941 gcgtgaatac ggcgcgctca gtgatataac gaacatccag ccctcgctcg agcgcgattt
    27001 ttccgctaaa agtaatttct ggtagttccc gaataaattt cttcacccgt ccttcctgga
    27061 cgatatttaa tttgccgtcg gtaatttctg tttttaaact gcccgcagtt aatgtgccgc
    27121 agaaaacgat tttcttcgaa gtggcactga tatcaataaa tccaccggtg cccatgattt
    27181 taccattgaa tttatgcacg ccgacgttac cgtgctggtc gacttcagca aaactcaaat
    27241 aacaaacatc cagaccacca ccgtgataaa aatcaaactg ggacgtcata tccagaatgg
    27301 cgcgggtatt cacgttcgcg ccaaaggcga tcccctgtga agtaataccg ccaatcggac
    27361 ctgtttctac cgtcagaata aagtcatcag cacaaccttc ttcccgggcg accaggccaa
    27421 tgccgtcagc aataccgacg ccgacattcc ccactgcgcc tttgcgcatt tcgaataatg
    27481 cacgccgtgc aactaattta cgttggttta ggggcaggct aagtttggtg ctgtcatcaa
    27541 gggtgaagtc accagaaata aagcggttaa ccggcgctcc gccatacagt tgggtttgat
    27601 ccggatcgac caccacaata tccaccagat aacccggaat acggacagat ttaggatgca
    27661 gcgtggcttt cttaaccatt ttctgcacct gcatcatcac aataccgccg ttattgtgca
    27721 ccgcctgagc aataaccaat gcgtcgagat acatcacctc atcttcaaaa gtggcgtagc
    27781 cttcgctgtc gcaggtggta gcgcgaatga aggcgatatc tggcgctatc gctttgtaat
    27841 agagatattc tttgttatca aactcgacca gtttaatcag gtcttcttta gtgacttcat
    27901 tcagtttgcc gccttgctgg cgtggatcga caaatgttcc gatgccaata tcactaataa
    27961 taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt aagtacacct tgtggatagt
    28021 tataagcaat aattttattt tgttctgcga gatcagaaat acgcggcgat tgtccccagt
    28081 gaccgcataa tgcccatttc accaggcctt cttgcgccag cggactaata ccacggtcgg
    28141 cgcgatcgcc aagccctgtt ggactaataa tcgataaatt acgtggtgtt tgagtctgtt
    28201 tatatttatc agcaagagca gtaattaacg tggtggtttc cagaataccg ccgccagcgc
    28261 ctaacacaca aagtgttgct tcgtcgggaa tataattcac cgcttcctgt gccgacagga
    28321 ccggcactcg tccattaatg cgaggtggtt ttacaggttt cattgttaat tcctgattca
    28381 ggtgtgattt tccc
//
LOCUS       DAZGTJ010000069        27933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000069 DAZGTJ010000000
VERSION     DAZGTJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.069"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(8..205)
                     /gene="safA"
                     /locus_tag="ACHK8E_003463"
     CDS             complement(8..205)
                     /gene="safA"
                     /locus_tag="ACHK8E_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP0345681.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            complement(453..2732)
                     /gene="ydeP"
                     /locus_tag="ACHK8E_003464"
     CDS             complement(453..2732)
                     /gene="ydeP"
                     /locus_tag="ACHK8E_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP0345682.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(3065..3979)
                     /gene="ydeQ"
                     /locus_tag="ACHK8E_003465"
     CDS             complement(3065..3979)
                     /gene="ydeQ"
                     /locus_tag="ACHK8E_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345683.1"
                     /translation="MDKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(4039..4542)
                     /gene="ydeR"
                     /locus_tag="ACHK8E_003466"
     CDS             complement(4039..4542)
                     /gene="ydeR"
                     /locus_tag="ACHK8E_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345684.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(4555..5085)
                     /gene="ydeS"
                     /locus_tag="ACHK8E_003467"
     CDS             complement(4555..5085)
                     /gene="ydeS"
                     /locus_tag="ACHK8E_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0345685.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(5099..7750)
                     /locus_tag="ACHK8E_003468"
     CDS             complement(5099..7750)
                     /locus_tag="ACHK8E_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0345686.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            complement(7792..8502)
                     /locus_tag="ACHK8E_003469"
     CDS             complement(7792..8502)
                     /locus_tag="ACHK8E_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0345687.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(8865..9428)
                     /gene="fimA"
                     /locus_tag="ACHK8E_003470"
     CDS             complement(8865..9428)
                     /gene="fimA"
                     /locus_tag="ACHK8E_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0345688.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(9862..10128)
                     /locus_tag="ACHK8E_003471"
                     /pseudo
     CDS             complement(9862..10128)
                     /locus_tag="ACHK8E_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(10404..11720)
                     /gene="hipA"
                     /locus_tag="ACHK8E_003472"
     CDS             complement(10404..11720)
                     /gene="hipA"
                     /locus_tag="ACHK8E_003472"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP0345689.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(11720..11986)
                     /gene="hipB"
                     /locus_tag="ACHK8E_003473"
     CDS             complement(11720..11986)
                     /gene="hipB"
                     /locus_tag="ACHK8E_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP0345690.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(12179..14068)
                     /locus_tag="ACHK8E_003474"
                     /pseudo
     CDS             complement(12179..14068)
                     /locus_tag="ACHK8E_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(14218..15810)
                     /gene="lsrK"
                     /locus_tag="ACHK8E_003475"
     CDS             complement(14218..15810)
                     /gene="lsrK"
                     /locus_tag="ACHK8E_003475"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP0345691.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(15889..16842)
                     /gene="lsrR"
                     /locus_tag="ACHK8E_003476"
     CDS             complement(15889..16842)
                     /gene="lsrR"
                     /locus_tag="ACHK8E_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP0345692.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            17091..18626
                     /gene="lsrA"
                     /locus_tag="ACHK8E_003477"
     CDS             17091..18626
                     /gene="lsrA"
                     /locus_tag="ACHK8E_003477"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP0345693.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            18620..19648
                     /gene="lsrC"
                     /locus_tag="ACHK8E_003478"
     CDS             18620..19648
                     /gene="lsrC"
                     /locus_tag="ACHK8E_003478"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP0345694.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            19648..20640
                     /gene="lsrD"
                     /locus_tag="ACHK8E_003479"
     CDS             19648..20640
                     /gene="lsrD"
                     /locus_tag="ACHK8E_003479"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP0345695.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            20652..21674
                     /gene="lsrB"
                     /locus_tag="ACHK8E_003480"
     CDS             20652..21674
                     /gene="lsrB"
                     /locus_tag="ACHK8E_003480"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP0345696.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            21701..22576
                     /gene="lsrF"
                     /locus_tag="ACHK8E_003481"
     CDS             21701..22576
                     /gene="lsrF"
                     /locus_tag="ACHK8E_003481"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP0345697.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            22600..22890
                     /gene="lsrG"
                     /locus_tag="ACHK8E_003482"
     CDS             22600..22890
                     /gene="lsrG"
                     /locus_tag="ACHK8E_003482"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP0345698.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            22947..23705
                     /gene="tam"
                     /locus_tag="ACHK8E_003483"
     CDS             22947..23705
                     /gene="tam"
                     /locus_tag="ACHK8E_003483"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP0345699.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(23709..24623)
                     /gene="yneE"
                     /locus_tag="ACHK8E_003484"
     CDS             complement(23709..24623)
                     /gene="yneE"
                     /locus_tag="ACHK8E_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP0345700.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(24820..26271)
                     /gene="uxaB"
                     /locus_tag="ACHK8E_003485"
     CDS             complement(24820..26271)
                     /gene="uxaB"
                     /locus_tag="ACHK8E_003485"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP0345701.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(26499..27917)
                     /locus_tag="ACHK8E_003486"
     CDS             complement(26499..27917)
                     /locus_tag="ACHK8E_003486"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0345702.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
BASE COUNT         7167 a         6822 c         6580 g         7364 t
ORIGIN      
        1 tttattttca gtcttgcaaa ctattgataa tgaaatgtgt aagatccctt gcaatgcgac
       61 ccatttctga aatacccagc tgatcaattg ccgaaaaaat ggctatcaac gtaagtgtca
      121 gtaataagac aaccacaatt acagacatga tttctttata gttgtctctt tgcgtgattt
      181 tgtttttcac tgtggtcgca tgcatttcaa atatgtttat ttagcggata acgttaaaaa
      241 tcgttaatca gtatgcttaa tataacaggc tggagaatag gggaaatagg actggcgctt
      301 cagtactgag cggagtttct tacagctgta ggcggaggta tatatcttat aacgagcagt
      361 ttaatttagc acatgtgagt aaattgcgcg acaatggtca atgaggaatt cttctgctgt
      421 gcggatgaca gcagaagaaa tgaggaggcg aattaatttg atggttctaa ttcaaccgga
      481 atacttttat agccaggaat gccacttaat ggatcgtggt tatcaagtgt tagcatgtga
      541 ttcgattctg gaaaataggt caccagtgag cggtcagcca tagggtaaat gaccactttt
      601 aatctatcca tgcggcgtga gctacgttta ccgtctggcg tgagcgcaat aagattaact
      661 ctttcgccgt tttttacacg gcaaatttta gcttgtttag cactcataaa gaccacatct
      721 cgttgaccga atacacctcg atagcgatca tccataccat aaatcgtcgt gttgtactga
      781 tcgtggctgc gtactgtcgc catgaccagt ttactgttaa acgctgaaga gggatcttct
      841 aacagccctt tactggtaat gaaattagcc ttacctgacg gcgtcatcca gcgcctttca
      901 gcagctgcat ttatcaggtg aaaaccaccg ggatgacgga tgcgctggtt atagtcggcg
      961 aactctggca gcacagcttc aatgtcattg cgaatgcgat cataatcttc caccagatac
     1021 tcccaggcta ccacgctctg gggtagtgct gcctgcgcga ttcccgcgac cactgcacac
     1081 tctgatttca gcattacacc ggcgggtttt aacacgccac gcgaggcatg aatcatcgac
     1141 attgaatcct caacggttac cgcctgcgca ccgctttttt gcatgtcaat ctcgctgcgt
     1201 cccagaaccg gcagaatata gctatgccgt gcggtcagca gatgagagcg gttaagctta
     1261 gtggctacgt gtaccgtcag atctaattgt gttaacggta cagcgcttgc ttcccgatct
     1321 ggcattgcca gtgcaaagtt gccccccatg cagatcaatg ctcgagcctg ccccgtacat
     1381 attgcttgca tgctggcaat tgcagcatgt ccaggtgcat gaggtggggt gaaaccatag
     1441 cgctcaccca ggcgagccag aaactctgca gacggtttct cggtgatacc gacggttcgg
     1501 tcgccctgta cattagagtg acctcgtagt gggcagatac ccgcaccagg cttaccaatg
     1561 ttacctttca tcaacagcag attgaccagt tgctgtacgt tctgggtacc atgttcgtgc
     1621 tgagtgatcc ccattccgta acagataatg gttcgttcgg cagcggcata tgcatcagca
     1681 agttcggcga tttgtgtctg acttagtcca gaaatacgtt cgatatcttt ccactcggaa
     1741 ttgagcacgt cacggcgtag ctcgtcaaag ccgatggtat gcgtttgaat aaattcgtca
     1801 tccagcaatg agggccgacc tgcggcgctt gcagcatcat cgcgctcaat taacaggcgc
     1861 atcatcccct tgagcaacgc catatcacca ccaatgcgca cgttgtagta ggcactggcc
     1921 aactgagtct cagagttcgt cagcatttca aacgggtttt gcggtgcggt aaatcgctcc
     1981 aggccacgtt cctgtagagg attgatggcg atcattttcg ctccccgttt cactaaagcg
     2041 cgcaacgaag tcaacatgcg agggtggttt gtaccagggt tatgcccaat gcaaatgact
     2101 aaatcgcatt tctcaaagtc ttccagcaac acggtccctt tacctacacc gatactcgct
     2161 gccaaaccca cactggtcgg ttcatggcac atgttggagc agtcggggaa gttattgctc
     2221 ccgtattcac gggcaaaaag ctgataaaga aaggcagctt cattggaagt gcggcccgaa
     2281 gtatagaatt caacctgatt gggatcacta tagctttgaa ggcgttcgcc aatttcgtcg
     2341 aaagcttgtt gccagcttaa tggcttgtaa cagtcgctga cgtcatcata tttcaaaggc
     2401 tgagtgagac gtcccgcagc ctcaagctcg tgatctcccc aggtaagtaa tgattgaacc
     2461 gtattctcag caaagaaaga ggcatttacc tgcttatccg tgacttccca ggcgattgct
     2521 tttgcgccgt tttcacaaat gtcgaatgac gcactgtgct taggatctgg ccatgcacat
     2581 cccggacagt caaagccctc tggcttattc atatcaaaca tggcaataac atcctggcgt
     2641 atatccatct gcttacgtac tgcatttgct acggatttaa cagcacccca accaccggca
     2701 gctccctggt aggattcaat ttttttcttc atctgcttat cctgtgtgga aatgaattat
     2761 taggatttgt aatagcgatg atgcacagta tatacaaagg gattatcgtc aacatggata
     2821 tataagagta ggggactact ctctttctat tttaatctat tatcgttaaa caaaagatta
     2881 aatacgataa attaagcatc atctctcata gtgtttaaat taacatattt ttcaatataa
     2941 aaaataagac tctggcgtta attgtgcgcg gattttctta caggtgtagg ataatagcct
     3001 tcacaaaata attatccgcg caattttcgc gaatggagcg catagactaa ggtcaaaatc
     3061 acaattattc atagataaaa gtgacaccaa tgactgactg aactgttccg gctgaaactt
     3121 tgtttcctgt ttgtccatag gttgcgctta aacccagagg tactttgctt ttatttactg
     3181 tccccaggga gacgttttct cccgtagcca gtatttttcc attacgcatc agagagacac
     3241 ccactcccga tgcttttgtt gcatctggtg ctgtgttagc aaacacttgt ctggcactgt
     3301 cagtggtagc acccgaaaga taaaatgaga gcttttgttc acttgagcaa tatacgccaa
     3361 ggggtatttc tgcgcttcca ggaaagtccg ggagattcac gttgacatta cgactatcaa
     3421 cggtgcagcc gcctgttggc ataacaacac tgttatttga aataatattc cacgtaaaat
     3481 tacgcggatt tccgcttcct aacgtggcaa ttttatacat atgaatacgg gcaataactt
     3541 cccctgcttt tatgacaact ccaccggcca ctccgactgg ggtaatatag agttttaacg
     3601 gtaatggcat cggtgtctta tcaccaatat ccaatacgtt ggtatttgtc gtcaacggga
     3661 aggggtaggt cacattattc cagtacagtg atcctttata tgactgtagg gagcctgcga
     3721 aagctgatcc ttgtaccagg tttatatgat cagtgtcgta ccagccgccg taatcattcc
     3781 agcaacttat atgctgagac aagtctacaa ccagattctg gcccggttgt attacaggat
     3841 cgagattaac ataaacagag gttgtacctg ctccaatact tgacccgcca tcgacattac
     3901 aactgaaggc aaaaactttg cctgccatta atagaagata aataccgaac aggaccttta
     3961 tactgaatgt tttatccatg atatatccta aggttaaaaa attgatttaa aaaggaggca
     4021 aaaaaaatca aattgatgtt attgccaggt atagatcaca ttgattagcg cctcgatcgt
     4081 tccctggctt gcgtttccat tcaccgtgat agctcttgcc ttaagtggaa actgtgcatt
     4141 acgagtgatc tcatcaacaa taaccgtttt gctatcgcca ttttttaacg tcgcatcctg
     4201 gtcatccctt agctctatct gaatattttc ggcagtacct tcatttttgt aataacccgt
     4261 attgtcagtt gaacctgtca cgattgccgt cactgcactt gtttcaaccg gacaatcggt
     4321 taatgacaaa gtaatattgt gccagcctga tgcagaacca ggttgttgca gattgcgcgt
     4381 ataaagatcc ccgagattaa cgttagcttc tttggtttga atagtgcagg gtttagcgac
     4441 tacccgacca ttaacagtga tagtgacatc ggcggcctgg agagttgctg tgaataacgc
     4501 gcaaaacgtc gctaacagga acctcttgtg aagtcttttc atatctggct ctgattattg
     4561 aaattcaagg gtaaaggttg ccgaagccct caccagtccc ggattgacgg acttctgagt
     4621 tgacttcaga cgagcggagt aaggcaaaat attgttctgt tctggtacca gtgggatcca
     4681 ctgcatcccg gcatgaaggt cattcaattt caccggacgc atatttccgt ccagtatttc
     4741 tatacccaaa ccggaggccg tgttgcttcc ttcatccagt ttcaacagag tattattttc
     4801 tgcatcctca ataccgttaa atgcaacccg aacccccgtt gtccctttac tgcattcact
     4861 taacgtaatc tgaaaaggga cggctggact tgtgctaccg gtcgttggaa attgtctggc
     4921 actgtttttt tggagatcta cggtaaaatt aagcgaatcc gatgagactg tgcagccata
     4981 atcgagaacg cgcccgctaa ttttaataac gctatctgca gataaagcag aataggtggt
     5041 taaccccaga cataaaccga ggaaaataac gttattgtat ttcataatct attgttcctt
     5101 agcgacagat tgctgtctgc tggttcagca aggtttcagg agagactgca ggaagcttgt
     5161 actcgacaat acagtttgag tttttatctt tgccccatga aacctgtaat ttccctgact
     5221 gtggaagtcc agtcagataa acctgaccgt tttccgcgac aatgctgcca tttttattct
     5281 ccccatgtgt gacaattgca ccgaatggaa cgctcttatt accgtacttc aacgtcatta
     5341 atactttccc gccgatttgt gcattaaatg ttgctctggc aatagcaccg tgagttggga
     5401 tgacagtgac aacggtttca tccagttcaa cattatctgc aagggaattc gcgttaagag
     5461 cgacacgatt ttctctatat tctgtcgcaa atggtaatat ggcatagcca cgccagtcgg
     5521 tatgaattcc ggtctggttc tctattttga cattatcagc gccaggagcc ttaaccagaa
     5581 ccattgtgtc gcccagcggc tgtccaaagg tgatgccatc agcatgagca ataatgccac
     5641 cactcattcc gtaataaatc tggctgctgt caccactccg actgtaaccg acattcgtat
     5701 taccgtaagc tccacgataa ttaagagaac tgtaaccact ggtgccagac gatgtattac
     5761 ccccttgggt gttaccgacc tgaacgctat aattcaggtt attatccggc agcagagtgc
     5821 cataaacccc tgataggttg gtcatgccgc ctttcaaatc gtttgacata ctgtaactgg
     5881 cgtttgaatt acgaaatgcc gactgactgt ctgtacgcat ccaatgactg aagggaacat
     5941 taagcgtaaa agcaagtaaa tgatcccgat cgttttgcca tatattattg gaatagctgt
     6001 aattcagcga agtcgtaata tcaccaaacg gcacatttaa tccaaatgat atttgctggt
     6061 cgctgcgtga cgtgttccag taactttggc gactggcact gaaaaatgtc gtaccgtaat
     6121 gtccaagctg ctgagagatg cttatttgtt cctgaccacg cttactgtaa aacagattaa
     6181 aataatcaat aaatagtgtc tgctcattgc tgtctccggt aggaggcttg acggtgtaac
     6241 cactcattcg actgtaggca ctgtcggata agttataaaa accttgcgta gaatagcgat
     6301 atcctgcgac ctggatattg gttcccgtct ggtagaagga tttgctgtag acggatttaa
     6361 ccgattgtcc gctgtgacgg gtgtcatctg caagcgtcgt attggcctgc gtgatatcga
     6421 aagaaaaggc accaaaaagt cctaaatctt taccaatacc aaggttgaag gcctgatatt
     6481 cttctgcaat ttgcattccg ccataaggtg tccagtttcc ttccagtcca tgcatcaagc
     6541 taccttgtat gaatttgggg gagctttgca ggttatttcc actacgatat tcccccatgg
     6601 caagcgcata acgcgtatat ccagcacgtt ggagaaccgg aacagacgaa taaggaacat
     6661 ataatgtctg aatactgccg tctgcatctt ttatggttac ttgcagatca ccaccattgg
     6721 ccgcagagtt gatatcatca atagtaaaag ggccgggtgg gacagtagtc tgataaacat
     6781 cgtatccatt ctgttttaca ctcacttgtg cggtgccgcg ggcaatacca tgaatcaccg
     6841 gagcgaaacc atgttggcta tcgggcaaca tcgcttctgt tgaatttatt ttgaggccac
     6901 gaaagtttac actatcaaaa atatcgccat cggtataact atcacctacc gttaagcgtg
     6961 agcgtaaggg aataatgtca cgttcagccg acgtattgat atgctgccat ttattgctgt
     7021 cagatgaatt gctactccca ctgttataac tccacgttga gttatcgcgc agtcgccaac
     7081 taccaatgtt gatgccactc tgtaaattca aatatgcata gttggatttt cctgcattat
     7141 ggttactacg attattaata gaattaccat taaaactata gtttagtagc ccagcattta
     7201 ttccttcttc ccacagatcc ggagaaacat agccgcgagc cattctgcca acataaattt
     7261 gcggaacgga gagtgataga tgttgctgac caacattaaa ctcagatgat gcgtcagcca
     7321 aacgatcccg aagtggtgtg catgcgctgt tgtccgacac atgtttttct gccgaatgtt
     7381 ccttattgtc taataatgca gattttttta tgccaagact gacaagaaga tctgtactca
     7441 ggcatgggat gagatctgcg ttattatcat ctgcaataaa agtaatatcc cgactagtca
     7501 taaattcgtc gttcaaataa atgtcgaccc gatatgtacc aggtggtgcc tcgtgtccct
     7561 tagtaaaggc ggataaatcg acagattcag caagatcatt gcttaaaaac cgtggattaa
     7621 aataatactc tgcgtcaacg ggaaaaaccg cagttgctaa gccaagggca atggtaagcc
     7681 gtgcaaagcg aggcagtagt aacacctgat gcatacgata tgccttaaat gcggcacgaa
     7741 aggctgtcat tattaattcc gattgagttt attattaatt aagtcactgc tttactgcat
     7801 ggttgcctta atacgcggag ttaatgcacc gtaatcattg atcgtctgaa aactgatatc
     7861 tccggatgcg gcatgcggga tatccaccga tacttcacct tttggcggca ccatggtgtt
     7921 tgggagattg ctatttcctg ctttcatatt cgttacggta atgaagtagg gcgttggatt
     7981 aataagagta agtttgctac cgctgcgacg aaaccgaagc attgcaggcg cttcttcagg
     8041 tgccattgcc aggttcgtgg ggcgataaaa catttttatg cggctaataa ttgccagctg
     8101 taatgtattc tcattcttct ggtctttttc catagcagga atagctttaa cattcaccca
     8161 gaacaggctt tcacggtcgc ctggtaactg atgattagtg gcgttgatta tccgcagcgt
     8221 gttctctttt ttaccttgca tggaaaaaag cggtggcgta atgacaaact gggtgtcttt
     8281 ttggtcaccc gcattttcta tccatgactg aatgagatag acactggcag gatcattatt
     8341 cgtgacaggc aataaaacct gtttctggtt tgccggataa attacccgcg tcgcacccag
     8401 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
     8461 cgccataaag gaaacagagt aggggggacg cgtagtttgc atctgatgca ttctccatgt
     8521 gttaagagga taagcgggga aaccgttaca tcagacaatc ggtaaatgtt gctaatacat
     8581 tggcgagtcg ccgggtttcc agacttaatg cgtttgaaat aaatgttggt tctcttttaa
     8641 taggaaaacc ttgctataaa gtaaatattt taaataacag taagtttaac tactgttgtt
     8701 tattgaacgt ttagatatta tgtgattttt tcataccgct ccaaaacaca aactattcct
     8761 cattaataat gagatgaaca ttggtgaatt tctcgggtgt gcgtagccat gccacacaca
     8821 ccctgtttca atttcagatg acgtatagcc atcttaagat tgaattatag atattgaact
     8881 ttgaaggtcg catcagcgtt tgcagtacca gctactgact gcccgagagc aatataacga
     8941 gcctggaacg gcaatatatt cgtgccatca atcaagtcag ttttagcgct aaaagtggca
     9001 ccatcaagta ttaatacctc ccccgtacgg tccaaaattt gaataccgac attttgggca
     9061 gagcctgccg ctgaactctg cagcgctaac gtatcgtcat tactggtgac agtagtaccc
     9121 aggaatgcaa cagctgcatt actggaaaca ttggtatcac aatcattcag cttgatattg
     9181 aagccaacgg cggagctgag gtcacccgct ttagccagac gagaagaacg gacctgaccc
     9241 agctcaaccg tttggttcat tgattctgaa tcaatcgcac aagcggcatt taccacttca
     9301 cctttaaaat gaataacacc gccattaaca gtggtcgtta ctgattcatc accctcggct
     9361 gcgcttaccg cagcagacga catcgccaat acagaaacga caatcatacc gacatgtttg
     9421 agtttcataa ttctccaaaa tttatattta agtagctgaa taaagagagt taacaattaa
     9481 gtcattctgt tattgcttat aaaacaagaa tcattcttaa ttggtagttg ccctgagaat
     9541 atagtttcca gggttattac atttcacgaa gaacgataat atctttaaat tacaaggaca
     9601 cctaatcaca acaagtttat tgcagcactt gttgtagaga gtatttgcaa agacatttta
     9661 tgtcgctaaa taacatgacg atttcattat gcatgttatt tataaaccgt gactaaacag
     9721 aatattattg cgaacaaggg aagtgtcagg aaaggtcaag agcgaccaat ggtttatatg
     9781 gctataaatc attagccata gttaatgttt gcctggtgta actaacacgg aattgctcta
     9841 aacagataag tgtagggttt attaaagctg accgacagac tttcttaacc tggtaatatc
     9901 tctcttaggt gactctccaa acatccgcga atattcccga ctgaaatggg ataagctttc
     9961 gtaaccgaca gcataggctg cagtggtgac atcgtaatgt tcattcagca tcagtcgcct
    10021 ggcttcattg aggcttagcc atttccgata ccggagtgga ctcattcccg caagctgtct
    10081 gaaatgttga tggaaagagg gggtgttcat ttgcacgcgg tttgccagcc tgactcttca
    10141 acgctgagaa gatgcggcaa acggcaaacc tgcataccag ccagcctata tccggtatgc
    10201 acaggccaat tatgaaagag gagaggcttt atgttctgga accttgaagt aataacgtcg
    10261 accggaaaat cactctgcat catcactcag attcaattta acataatata cattatgcgc
    10321 accaacataa accaagggag aatccagccg ttggcggtca tgattgtcct gctcattaac
    10381 aacggccaca ccccatatct tacttaatac cattctcggc ttaaccgacg gtgcaacctc
    10441 aacacattgg tttcaactgc cgtcaccacg ttctccggaa aatctgtcgg taatgaagtc
    10501 ttcacgttat ccagtgctgc cggaatcatt ctggcaaagt cactcaggat ttcatgcatc
    10561 tgcacttgcg gaaatttcag cacctttgct gtcgccagaa aatgtcgcgg ataaatttta
    10621 tcgattgccg tttttttgcc tttggatgcg ttaagcccca ttgccagttt gagatcgctg
    10681 atgtgtattc ctgcaccacc caataccgga aatgctgaaa tgatgtcgta aaatggcgtg
    10741 agtcgataac tgccgccagc ctgaataaat acggagaagt tttttgcatg gccatccgtt
    10801 gcgccaatca accactggaa cacctgaaac ttcataaaat catagcgatc tttcagcgcc
    10861 tcgctggacc ccatcaaaaa agccatgatc cgcgcgatgc ctgggcctcc atctgattca
    10921 tatttcaccg atgaaggtaa accgaatgtc tgacacatat cctcctgtgg caagcgaagt
    10981 aaaaccgttc gctcagcatt ccaacgcctg tcaaaacgtt cgaccgctaa cgcgcgcaca
    11041 tttcccgctt taatgatttc tgcgtccggc acattcaacc caagttcttt agccagcagc
    11101 agacagtaat actcattatc aacgctttgg ctgagatcga gcgtcgcatt gggctgcctg
    11161 atttcgccaa tcggtaattt aatgatgtgc gtcgtcggcg ttattccttt cggaatgcac
    11221 cagtcattgc ctattctgag cagcgctgtc ttctcctgcg cgccagcaac cgagatgcga
    11281 aagtcatttt cttctctaat catgcccaga gggatatctg ctttataagc cgttaatact
    11341 tcttcaagcc tggcttcagt gagctgttcc catgccatta tcgggcgcgt tatggtttca
    11401 ttttcgggta ataacgttac ggcaccaacg ctgtctcgcc ctatttctga caataaatca
    11461 aacggttgtc tggatttagc atgataacgt ttaacgatcc gatcacgtac aatcgggcta
    11521 tcgggtaaca ggttatcgaa gaagttaaat acggcatcag aggtgatatt ccccctctgc
    11581 aatggcagcg aaagtgacaa cggtctggca taacggctgg ctaaccactc cggtgcatac
    11641 ttaaaggtgt gcgcgccatt ggctaacttc gttaactcgc ctacccgctg gttgttcatc
    11701 caagtgacaa gtttaggcat taccactcca gatcttgctg ttctgttgat tctggcgagg
    11761 catttttcgt gtcgcatagc gtcattgaga gttcaagcga ctgtaaaatc ttaaaaaagg
    11821 tcgtcagcgt ggtattgtca gggttgtttt cgaaattgga aatcgtcgcc tgcttaatac
    11881 caattttttt cgccagctcg ctctgcgtcc agccattttg ctggcgaacc agtttcattg
    11941 cattcgccaa ttgcgttggg ctatagatct tctgaaagct catcatgtca tacgtccaca
    12001 gcaaatttat ccgcttaagg ggatattatc agttttatcc tttagtgagg ataagtcaat
    12061 ctggaaacac atcttatccg ctctacggga taaacactgt gatatccgcg atcgcggata
    12121 aaaaggagga tttaaaagtc gtttgaagtc ggcaatatta agccgcatgc atcttcgaca
    12181 tgcggctatt cccttgggta tcgcctttta cgttcatctt atcggtgggc gaattatcgc
    12241 cgcccagcgt cgcgttgaac gtcatcaggc cattgtttcc ggtatagtta ccgttaacgg
    12301 tcagtacatt ccccggtttc cccccttcgc tgttcatttg aaccgtgcca ccattctcca
    12361 ggttaccgcc gacagtggtt ttagcgacac gcagtgtccc ccctggcata acgttcacat
    12421 cacctgcagt ggtgacattt cccgacatca caccgctgtt atggatatta atgtgctgac
    12481 ttgccatatt aatggcagag tcggaaccga aagcaatttc accacccacg atatccgtcg
    12541 tacccttata cgccgcggaa tctttgttaa gcgtcaggat cccggtaccc agttttgtca
    12601 catcaccgct accgctgata ctgtgcgtca tcacccagct acgctcagtg gagatggtga
    12661 gcaggccatt attgataacg gaagcatcac ccaggttttt ctgctcgttg acgctaagcg
    12721 cgctgcctgt atcaatgttg aattgcccca caaagcggct attatcgcca tccagctcta
    12781 cgtctgtcct ggcggttgcg ctcacgatac ccttcccgct tatgctatta cgcagttcgc
    12841 cggtcacatt ttttagcgtt aacagaccgt cattggcaat atttcctctg cctaacccct
    12901 gcgtattatc gaggctgact tccgcatttg ggctaatgct ggttaacgca ttgtagcgtg
    12961 ccttaagccc ctcgatagcc agtgttcccc ctgtaacatt caggtttccg ccacctgtca
    13021 gttcgccctg actgatccca cctttattca ccgtcagcga cccctcttta aacaaaacag
    13081 tcgaacccat ttgcccggtg aatgtctcta ccgtctgggt cgatccatta agatcgacga
    13141 tggccgcgtt gctgatgttc agttcccggg tgttgcccag cgcgccatcc gcatcggtac
    13201 gtaatgtccc catctgaacg taggttgccc cctgatagtc gttctgaccg ttggaaagcg
    13261 aaacctgtcg taccgtattg attgccagat ccccttcgcc accgattttt gccgacatat
    13321 cgaccgttgc gccataagct ccgccatgtt cggctaacgt cagcttttgc ccaccatgga
    13381 tgttcagcgc tttcagccca tagttaacat acaaaccatc accaggtgcg gtcgtgaggc
    13441 ggaatccata atcaccctcg gcgaccaccg ttccgttttg cgcaatatgt aacgttttgt
    13501 ccgcttccac ctcgtcgccc tgtaaatcac gtaacgttaa tgaaccaccc gatccaataa
    13561 ccgtttgcgc cttcaccagt tgaacgccga catggctatc gtcgtgttcc atcaaattga
    13621 gcgtcgtcag agggttattc gccatgggat cattccacgg tttaggcacg tcgataagca
    13681 cgtcgcccat cccgtttacc tgatagttac ggcctttcca ggtgtagtca cccgcgccga
    13741 caaccagcgt atcgacgctg atatatccct ccgcaagcgt cgcagcaggt atatccgtat
    13801 cgaacgtgag cgtaccgcca ttcatggcta gtccaccaat ggattgctct cccactttta
    13861 ccgatgtggt attttcactg tcagactgca gcatcgcgta agtaagcgca gcggtgttgt
    13921 cgcgttccag tgcgaaggta ctggctttca gttgggcaac accggcgaat tcagtccctg
    13981 ctgcatgggt aaagccaaac attttgtcgg aggatgacag ctgcactcgc atcaagccat
    14041 cgcctttgag cgcattgatc agcgtgaata gcaatttccc cttgaatata gtactggtga
    14101 tggaacgatg aatctgcaag ctggcattta acaagccagc tctaaaagaa gggaaatgag
    14161 aataccaata ctcaaaaact aacagccacg gtcatcatgg tgtggctgtc aatgaaacta
    14221 taacccaggc gctttccata acgacgtcgt cagtccatga tcaaccagcc ccagctgatc
    14281 ctgataaact gcctgccact tatcgcgtga atcctgataa agttcatgct tttccgggtc
    14341 tggtgtgtgc gtccgttccc aacgaaccaa gcgttctccg gtttctgcca ttgatgaaaa
    14401 aattccggca ccgacgccag ctgcaatggc acatcctaat gcagtggctt ctttgaccac
    14461 cggaatattg acgggtaatc ccgagacatc agcgagaatt tgactccata atttcccttt
    14521 tgaacctccg ccagcaaaaa ccagcgaggt gggatgaata ttcgagaaat cagcaatttg
    14581 ctgtaagtta cacgctgata caatcgccgc attttcttcc agcgcacgga acaatgtcgc
    14641 tttgttacat ttatccgggt caatggacaa gttaataaag gaaggcgcag cgtgatacca
    14701 ggttttaaag cgcattctgt cggagaatat cggcattacg ccccacgacc caggcggcac
    14761 ccgactggcc atctcttcca gcagcgtata ggtgtcgatg cctaaacgtt cggcaatcag
    14821 tttttcttcg gcacagaaag catcgcggaa ccagcgcatg gtgagtccgg taaaaaagct
    14881 tatagattca gcttgtacca tgccaggaat aacatgggga ttaacgcgca cgttcatttc
    14941 tgggtctgtc accggcgcgg ctaagtttac aacttgctgc cagaatgtgc cgccaagaac
    15001 cgcggtttgt gccggacgta caacgcctaa ccccaggcaa ccaagctgca cgtcacctcc
    15061 tccaacgacc accggagtgc ccgccttcag accgcagagt tccgccgctt gtgaacttac
    15121 cacacccagc aatgtgccgg tttctttgac aggagaaaga atatcggcac gcaggccagc
    15181 catatccagc aatgcaggtt tccagtcacg ggtggttaga tcaataagtc ccgtggtgcc
    15241 agcgttagag ggatccaccg caagttcgcc gctgagcata taggccagcc agtcgctgat
    15301 catggtgatg gttgatgcct gacggtaaat atcggaacga tggtgcgcca gccaaagtaa
    15361 tctggggatg gcgcttaaag ccagcgtttg cccggtcgct cgataaactt tgttttcaaa
    15421 ggtattgttg tgcagttctt taagttcgtt aacttcgcgc gccgctctgg catccacatt
    15481 ggcgcaggcc cagatcggtg ttccttcatt gttatataaa acaatgcctt cacgcatcga
    15541 acatgccgaa acagcagcga tatactccgg ggctatgccg gcgttgtgca gcgcctggcg
    15601 catacactca cacgccagtt gccagttttt gttgagatca aattccatag aaccaggaac
    15661 gtccggtact gccagatgcc gccactccgc ctgtcccacg gctatttgat tgccttccgg
    15721 gtcgaatatc acagcccgaa tacttccggt gcctgcatcc agcgccatca ggtagtactt
    15781 tgattctgaa ggggtaaaga gtcgagccat tagattatcc tcggcttatg cgttctgtct
    15841 gttcctctat acgttctcca tcattcccgg taacaaggtc atgcaaattt aactacgtaa
    15901 aatcgccgct gctgtgtcct gatcggtaac cagtgcgttg atatagccgc ctttcattgc
    15961 agcggcaatg gcttcggctt tattttctcc ccctgccacg ccaacccgga cgggtatggt
    16021 cttcagcgcg cttaaaggta agccaatcag ttcgttatgt attttaatat ccgtgacaac
    16081 gtcacctttt gcatcaaaaa agtagcctaa aatgtcgcca acagcccctt ttcgaccaat
    16141 cattaactgt tcgccctggc tgatatagcc ggagcgaatg attgttgcgt cgtcttgttg
    16201 actcacagcc ccaatgccga caatcgccac atccgctgct tgcgcggcta gcagaacatc
    16261 tttgacgcaa ttttcatttt ttagcgtacg ggcaatgtca gcggaggatg cccgcaacgg
    16321 agccggaata atattcacac tgcacgccgc gttaagctgc ccgatacccg tcatataaga
    16381 accgactcca ccggagagcg tgaccaggcg aatttgctgt gacgaaataa aaccacttaa
    16441 gcgttgcagc gtattcatag ttgcctcgcc aaaaccaatc gccagcatct gttgtggttg
    16501 aagtaaactc atcaacatat gcgccgcgcc tatccctagt cgcccgccga catcagcatc
    16561 cgcaagccct gggatcaccc ggacatgttg cagcgaaaac tgacgacgta attgtgtttc
    16621 atattccaga cagccttcaa agcgagaatt aatctgtacg cgaataatgc cggattgatg
    16681 ccctttctcc agtaatcgcg acactttcaa acgggtcagg ccgagacgat cgctgatctc
    16741 actctgggtc agcccgtcgt ggtagtaaaa ccacgcaatc cgcgcgacct gttcttcttc
    16801 acacattccc tgttctgaaa ttaccgaatc gttgattgtc ataattcatt cttcactttg
    16861 aacatattta aatctttaat gcaattgttc agttcttgcg catttatatc tgtgatagca
    16921 accacagttt gactctacga gcatgaacaa acgcaaccgt gaaaatcaaa atagcataaa
    16981 ttgtgatcta ttcgtcggaa atatgtgcaa tgtccaccta aggttatgaa caaattaaaa
    17041 gcagaaatac atttgttcaa aactcacctg caaaactgaa cgggggaaat atgcaaacga
    17101 gtgatacccg cgcgttaccg ctactttgcg cccgctcggt ttataaacag tattcagggg
    17161 ttaatgtcct gaaaggcatc gattttacgt tgcatcaggg ggaggtccac gccctgctcg
    17221 gcggcaatgg tgccggtaaa tcgacgttaa tgaagattat tgccggtatt acccctgctg
    17281 atagcggtac gctggagatt gggggcaata attacaccag attaacgcca gttcatgctc
    17341 atcagctggg catttatctc gttccccagg aaccgctgct ttttccaagc ctgtcgataa
    17401 aagaaaacat cctgtttggt ctggcaaaaa aacaactctc catacagaaa atgaataact
    17461 tgctggtggc gctgggctgc cagtttgatc tgcatagtct ggcaggatcg ctggatgtcg
    17521 ccgatcgcca aatggtggaa atcctccgcg gcctgatgcg cgactcgcgg attctgatcc
    17581 tcgatgaacc tacagcctcg cttacccctg cggaaaccga acgtttgttt cgtcgcttgc
    17641 aagagctgct tgctactggc gtgggtattg tttttatctc gcataagctg ccggaaattc
    17701 gccagattgc cgatcgaatt agcgtgatgc gcgacggaac catcgcctta agcggcaaaa
    17761 ccagcgaact gtctaccgac gacattattc aggccatcac gccagcgcta cgggaaaaat
    17821 cgctctctgc cagccaaaaa ttatggctgg aattacctgg caaccgccca caacatgccg
    17881 cgggaacgcc ggtgctgaca ctggaaaatc tgaccggcga aggtttcagg aatgtcagcc
    17941 tgacgctcaa tgccggagaa attctgggct tggctgggct ggtgggagcc ggacgcacag
    18001 aactggccga gacgctctat ggtctgcgta ctttgcgtgg cggacgcatt atgctgaatg
    18061 gtaaagagat caatagatta tccaccggag atcgtttact gcgcggtctg gtttatctgc
    18121 cggaagatcg ccagtcatcc ggacttaatc tcgatgcttc actggcgtgg aacgtctgcg
    18181 cccttactca taaccttcgt ggattctggg cgaaaaccgc gaaagataat gccaccctgg
    18241 aacgatatcg ccgggcgctg aatattaaat ttaaccaacc ggaacaagct gcgcggacct
    18301 tatccggcgg caaccagcaa aaaatcctga ttgccaaatg tctggaagct tcgccgcaag
    18361 ttttgattgt cgatgagccg acgcgcggcg tggatgtctc ggcgcgtaat gatatctacc
    18421 aactgttgcg cagcatcgcc gcacaaaatg tggctgtgct gcttatctcc tctgatctgg
    18481 aagagatcga actgatggca gatcgcgtgt atgtgatgca tcagggcgaa attgcccact
    18541 ctgcactgac cggacgcgat attaatgtcg agaccattat gcgcgttgcc ttcggagata
    18601 gtcagcgtca ggaggcgtca tgctgaaatt tattcagaac aaccgtgaaa tcacggcact
    18661 gctggcggtg gtgctgctgt ttgtattacc cggttttctc gaccgtcagt atttaagtgt
    18721 gcaaacgctg accatggttt atagcagcgc gcaaatcctg atcctgctgg caatgggcgc
    18781 gacgctggta atgcttacgc gcaatattga tgtttcagtg ggttcgatta ccggaatgtg
    18841 cgcggtgctg ttggggatgt tactgaacgc ggggtattca ctacctgttg cttgtgtcgc
    18901 gactttactg cttggtttgc tcgcgggatt tttcaacggt gtcctggtcg cgtggctaaa
    18961 gatccctgcc attgttgcca cccttggcac gttagggttg tacagaggca tcatgttgct
    19021 gtggactggc ggtaaatgga ttgagggatt acccgccgaa ctgaaacaac tctccgcccc
    19081 gctgctgttt ggcgtttccg cgattggttg gttgacgata attctggtgg catttatggc
    19141 ctggctgctg gcaaagacgg cgtttggacg cagtttttat gtcacgggcg ataatttaca
    19201 gggcgctcgt caactgggcg ttcgtacgga agccattcgc attgtggcat tttcgttgaa
    19261 cggctgcatg gcggcactgg cgggaattgt gtttgcttcg cagattggtt ttatccccaa
    19321 ccagaccggt accgtgctgg agatgaaagc aattgcagcc tgcgtgctgg gcggcattag
    19381 tttgctcggc ggttccggcg cgatcatcgg tgcggtactc ggcgcatggt tcctgacgca
    19441 gatcgatagt gtactggtgc tgttgcgcat tccggcatgg tggaatgatt ttatcgcggg
    19501 catggttctg ctggcggtgc tggtgtttga tggacgcctg cgttgtgcgc tggaacgtaa
    19561 tctgcggcgg caaaaatatg cccgctttat gatgccaccg ccatccgtta aacccgcttc
    19621 gtcaggtaaa aaacgggagg ccgcgtaatg cgtattcgct acggttggga actggctctt
    19681 gccgcactgc tcgttattga gattgttgca tttggtgcaa ttaacccgcg gatgttagat
    19741 ctcaatatgt tgctgttcag caccagtgac tttatctgca ttggcattgt cgccctaccg
    19801 ctgacgatgg tgattgtcag tggcgggatc gatatttcgt ttggttcgac catcggcctc
    19861 tgcgccattg cattgggcgt actgtttcaa agtggtgtgc cgatgccgct ggcgattctt
    19921 ctgaccttac tgctcggcgc attgtgcggg ctgatcaacg ccggattaat tatctatacc
    19981 aaagttaacc cgctggtgat tacgcttggc acgctgtatc tgtttgccgg aagcgccctg
    20041 ctgctttccg ggatggccgg agcgacgggg tacgaaggta ttggtggatt cccgatggcg
    20101 tttacagatt tcgctaacct ggatgtgctg gggctccccg ttccgctgat tatcttcctg
    20161 atatgtctcc tcgttttctg gctctggctg cataaaaccc atgccggacg taacgtgttt
    20221 ttaattgggc aaagcccgcg tgtggcggtt tatagcgcga ttccagttaa ccgcaccttg
    20281 tgtgcgctct atgccatgac ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg
    20341 tattttggtt cagcacgttc cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc
    20401 gtagtgctcg gcggtgccaa tatttatggt ggttctggtt ccattatcgg caccgccata
    20461 gcggttttat tagtgggata tttgcaacaa ggtttgcaaa tggcaggagt gccaaatcag
    20521 gtgtccagcg ccctttccgg tgcgctactt atcgtcgttg tcgtaggtcg ttccgttagc
    20581 ctgcatcgcc agcaaattaa agagtggctg gcgcgtcggg ccaataaccc attgccataa
    20641 aggatatctt catgacactt catcgcttta agaaaatcgc cttacttagc gcgcttggca
    20701 ttgccgtaat ctctatgaat gtgcaggcgg cagagcgtat tgcatttatt cccaaactgg
    20761 ttggcgtggg attttttacc agcggtggca acggcgcaca acaagcgggt aaagagttgg
    20821 gcgttgatgt gacctacgac gggccgacag aacccagtgt ttctggtcag gtacagttga
    20881 ttaataactt cgtcaatcag gggtataacg ccattatcgt ttctgcggtt tcgcctgatg
    20941 gcttgtgtcc ggcactgaaa cgcgccatgc aacgtggtgt gagagtgctg acctgggact
    21001 ctgatactaa accggagtgc cgctcttact acattaatca gggaacgccc gcccagttgg
    21061 gaggtatgtt ggtggatatg gcggcgcgtc aggtgaataa agacaaagcc aaagtcgcgt
    21121 ttttctactc aagccccacc gttacggacc aaaaccagtg ggtgaaagaa gcgaaagcga
    21181 aaatcgccaa agagcatcca ggctgggaaa ttgtcactac gcagtttggc tataacgatg
    21241 ccactaaatc gttacaaacc gcagaaggaa tattaaaagc ctatagcgat ctcgacgcca
    21301 ttatcgcccc cgatgccaat gccctgcccg ctgccgcaca agccgcagaa aacctgaaaa
    21361 atgacaaagt tgcgattgtc ggattcagta cgccaaacgt gatgcgtcca tatgtggaac
    21421 gcggcacggt gaaagaattt ggcctgtggg atgtggttca gcaaggcaaa atatcagttt
    21481 atgtcgcgga tgcattattg aaaaaaggat caatgaaaac gggcgacaag ctggatatcc
    21541 agggcgtagg tcaggtggaa gtctcgccaa acagcgttca gggctatgac tacgaagctg
    21601 atggtaatgg catcgtactg ttaccggagc gcgtgatatt caacaaagag aatatcggca
    21661 aatacgattt ctgatgtgca ttacttaacc ggagtacgtt atggcagatt tagatgatat
    21721 taaagatggt aaagattttc gtaccgatca accgcaacaa aatatccctt ttaccctgaa
    21781 aggttgcggt gcactggatt ggggaatgca gtcacgctta tcgcggatat ttaatccgaa
    21841 aacgggtaaa accgtgatgc tggcctttga tcatggttat tttcaggggc cgactaccgg
    21901 acttgaacgc attgatataa atatcgcccc gctgtttgaa catgccgatg tattaatgtg
    21961 tacgcgcggc attttgcgca gcgtagtccc cccggcgacc aataagccgg tggtactgcg
    22021 ggcgtcaggt gcgaactcta ttctggcgga attaagtaat gaagccgtgg cgttatcgat
    22081 ggatgatgcc gtgcgcctga acagttgcgc ggtggcggcg caggtttata tcggcagcga
    22141 atatgaacat cagtcgatca aaaatattat tcagctggtc gatgccggaa tgaaagcggg
    22201 aataccgacc atggccgtga ctggtgtggg caaagatatg gtgcgcgatc agcgttattt
    22261 ctcgctcgcg actcgaatcg ccgctgaaat gggggcacag attatcaaaa cttattatgt
    22321 cgataaaggt tttgaacgga ttgttgccgg atgtccggta cccattgtta ttgctggcgg
    22381 taaaaagtta ccggagcgcg aggcgcttga aatgtgctgg caggctatcg atcagggcgc
    22441 ttctggtgtg gatatggggc gtaatatttt ccagtctgac catccggtgg cgatgatgaa
    22501 agccgtacag gcggtggtac accataacga aacggctgat cgggcatatg aactctatct
    22561 gagtgaaaaa cagtaactgc ggatttaagg agaagaatta tgcacgtcac actggttgaa
    22621 attaacgttc atgaagataa ggttgacgag tttatcgaag tttttcgcca gaaccacctg
    22681 ggctctgtac aggaagaagg caatttgcgc ttcgatgtct tacaggaccc ggaagtgaat
    22741 tcgcgctttt atatctacga agcctataaa gatgaagacg cagtggcatt ccataaaacc
    22801 acgccccact acaaaacctg tgtcgcgaaa ctggaatctt taatgaccgg gccgcgtaaa
    22861 aaacgtctgt tcaatgggtt gatgccgtga ggcgaattta tcagttttat ctacaattgg
    22921 ggtaacgcgc tgacgggagt aaataaatgt ctgactggaa cccctcttta tatctgcact
    22981 ttgccgctga acgatcgcgt ccggcggtgg agctgcttgc cagagtgccg ctggagaata
    23041 tcaaatacgt tgccgatctt ggctgtggcc caggtaacag caccaccctg ctgcatcaac
    23101 gttggcctgc ggccaggata acaggcatcg actcgtctcc ggcgatgatt gctgaagcgc
    23161 gcagtacttt gccagactgc cagttcgtgg aagcagacat ccgcaactgg caaccggaac
    23221 aggcactcga tctgattttt gctaatgcct cactgcaatg gctgcccgac cactacgaat
    23281 tgttccctca tctggtttct ttacttaatt cgcatggtgt gttggcagta cagatgccag
    23341 ataactggct agagccaacc catgtgctca tgcgcgaagt cgcctgggaa caaaattatc
    23401 cggatcgcgg gcgtgagccg ctggctggcg ttcatgctta ctacgatatt ctgagtgaag
    23461 ccggatgtga ggtcgatatc tggcgaacca cttactatca ccagatgccg tcgcaccagt
    23521 cgattatcga ttgggtgacc gccactggat tacgtccgtg gttacaggat ctgaccgaga
    23581 gcgaacagca gcattttctt acgcgctacc atcagatgct ggaagagcaa tatcccctcc
    23641 aggagaacgg acagatattg ctggcatttc cgcgtctgtt tattgttgcc cgccgtacgg
    23701 agtaattatc acgtcagctg gtaatggcga tcgggaagaa ttttcgctgg aatttctgct
    23761 tcatcgttca tctgtaacag gtcaatttca atagcgttgc agatggcatc cagcggtaag
    23821 tcattgtttt cagtaccgaa gggatcttcc agttcttccg ccagacaatc cagcgaaata
    23881 aaagtgtagg aaatcagcac agagataaaa ggcgtcatgt aatgcaggtc cacgaccagc
    23941 gcaaacggca gcatgataca aaacagatac acggtgcgat gcaaaatcag ggtgtaggca
    24001 aagggaattg gcgtataggc aatgcgctcg catcctgcca ggaccactga aatatcatta
    24061 agccgatcgt tgaggctaat aaacaggata tctgaaagtt gtccattgcg tcgctgaacc
    24121 gccaaccact cccccattat taacaagatg cggttagctg gagagttaga agccagtaca
    24181 cgctgaagat cttcagtctt gagataatga gccagcactt ccgcctgtgg ctgtttgcgt
    24241 aatgtcatgc gtaaacagtg ggcgaaggcg atttgcagcc gggcaaactc ccttacactt
    24301 gccgaatccg gcaatgtcgt ttttacctcg cgcagtaacg accgcgaggc aatcatcaac
    24361 tgcccccaaa gttttcgcgc ttcaacgtaa cgagcgtaac cggcattatt acgaaaacca
    24421 agaaaaatgg cgatggcgac accgagaatg ctgaacggtg cgagggtgaa tttaatgccc
    24481 aaatgcgtgt accagggcag catgaaaata acagcgatag aaaagagaaa attgagtagt
    24541 aagcgcgagg atatcttgga taatactgaa ccgtgccaga caaaaatacg gcgcagccag
    24601 tgttgttgtg gacgaacaat catggttatc ttcaggcgtg gaaaagtcgc cctattaaac
    24661 gtgattacga tcacattctc aagacactac ttacaaatta cctacaagct tacaacatta
    24721 acaggtgctg catgtggcac ctgttaatga taaaggcgca taggatgttg taactaatat
    24781 agtgaaataa gaaacccggt cgaaaccggg ttcagagtat tagcacaacg gacgtacagc
    24841 ttcgcgcatt ccttttttga gaatcgcatc caggtcatta gccacctgct cgaccagacc
    24901 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaacacaa tcgctaccag
    24961 ttcctgggta ccgataacac ggtcacgatg ctggctccag agttgctgat aacgttccag
    25021 ccagtgtgca tcgtcctgta ccggatatgt ttctccatta cgctcaccgc gataaaacgc
    25081 aatcaatgcc gctaatgcga aagtcaggcg cgccggaagt gtgccgtttg ccttctgccc
    25141 tgccagcagc tgcggcagga tacgggtgcg gaacttggtc ataccgttaa gcgcgataga
    25201 cagcagctga tgcttgatgt acggattacg gaaacgtccg gtgactgcac tggcgaaaga
    25261 ttccagttca tcacgaggca aatccagtac cggaataatc tcttcgtaga tggctttttc
    25321 aacgaatgcg caaatttcag catcgttcat cgcttcacct acggtatcca gtcctgcctg
    25381 gaacgccacc ggcaccagcc cggtgtgcgc accgttgagg attgccactt tgcgctcttt
    25441 atacggttta atatcgtcaa caatcagcac gttgagtgga tatttgtcca ggcgcagctc
    25501 agtcgctaag gatttcggcc cctgaatcac aaacaggtaa aagtgttcag cggtgtcgag
    25561 gaaaccatcg tgataaccca gttcttcttc cagtttagcc acttcatcgc gcggataacc
    25621 ggtaacgata cggtccacca gcgtagaaca gaagctgtta gcctgatcca gccattgaat
    25681 aaatgcttct ggcagtgccc actcttgcgc atagcgcaga accagttcac gcaacgcgtc
    25741 gccattgtag tcgatcaact cacacgggat gatgatccaa cctttatcca gcgcaccgtt
    25801 gaaatggcta aaacgttcga acagcagacg ggtcagtttt gccggataac ttaccgctgg
    25861 cgcgtcatcg aatttatcgc ccgcatggta gctgataccc gcttcggtgg tgttagagaa
    25921 aacaaagcgc atttccgggt tgtgcgccag tttcaggaat tcatcgtatt cactgtagac
    25981 gctgatttca cgattaacag agcgaatcag acgcgcgtcg ctgaccgctt cccccttctc
    26041 attcaggcca cggataatgg tggtgtacag accatcctgt gtgctcagtg acggtgggaa
    26101 tgaagtttca atcggacgaa caacgaccac gccagaattc agatcggtgt gctcattcag
    26161 gagatcaatt tgccagtcaa caaaggcgcg caggaagtta ccttcaccaa actgaatgat
    26221 acgttctgga tactgtgcac cgggaaaatc gcgacgattt agtgttttca caatgggttc
    26281 ccttctgatt agtcatacaa cctgttttaa ttggtacgac aggttagcaa actttaatac
    26341 gccgaacccc tgttttgatc aactcctgat gattaatgag cagttttatg agaaaagtgt
    26401 ggcgcggatc atggtttaat cgaggaacta cgccttttat tcgctcaata aagtggtaga
    26461 acacattccg ttaaaatcgc tgtgaatgaa taaagatgtc agacaacttc ctcaccgtaa
    26521 cgcatagtgc tggtacggtt gcgcccatct ttcttcgagc gatacaaaca tgtatcggct
    26581 tcgaccatca atttattaaa gtcatcggtc agcgttcggt aggatgcgca accactaccg
    26641 acgccaatac ttaccgtgag ataaagcgtt ttttgttgcc aggtgaacgg ttgcagttca
    26701 acgcctttac ggattttttc cgccattagc agaccatcga caggattcac cgatggcact
    26761 gcaacagcaa attcttcgcc gcccattcgc gccaccagcc ccttatcacc gacaatcttc
    26821 tgaatatgct gggcaaacac gcttaacact ttatcgccac attcatgtcc atagttatcg
    26881 ttaatacttt tgaaatagtc gatatcaagc agcatgacag tcagatgttg tgtctgtttc
    26941 agcgatggac ttttcagcgc ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa
    27001 tcaaagtcgg ctcgcagcgc aacttgcttc attagcgaat tgatcgctgc cacgctaaaa
    27061 gaaaccataa ttgggcatat cgccatcgtg gcaataccga gacgtgcgga gaacatttgc
    27121 ggaatggaga acggcgaacc gaccgagata tcaatcaccg aatttgccac cagtacgatt
    27181 tccaccgcac cggtgacaaa ggtcaacaga catgttacct gcggcgtata gcgcactgca
    27241 caccagatta atgcaggcag cgggaacgcc agactccccg ctccgccaat gactaccgag
    27301 gcaataaccg acacaataag cgcgatagcg ggcatcatct gttctgcttt aaagcgcggc
    27361 agcactccag gaattgccaa cgtcagcata caaggcacga tcaacacgcc cgttgagaat
    27421 tgctcactga accagtccgc aagcaaaggc cagaaatcca gactatcaat actgaccgaa
    27481 ccaatcgccc cgacaatagc gcataataat gcggcaatca gacagtaatt aaatagccgt
    27541 aaggcactta ctggctcata cttatttttt cccagacgct tatcccgcgc gaccagtaag
    27601 gcgacggtaa caataaacat catattggag aaattgatag ccagtgaaac cagcccccat
    27661 tcggtggtta tggcatcata aaccagcatc gccacataac tgatcgcata gtaatgcagt
    27721 cgattaagcc agacatagcg ggcaaacacc cccgccatta cgccattcag cggccagaaa
    27781 agagaaagtt cgtgaaccag ccgtaactct gcaccaataa aatagaacag cgtggttagc
    27841 acaaaaatag cgatgctatt acgtagcaga tgaccttcct ggaacaaacg gaaagttgaa
    27901 atgggctgta catgcattaa ttatctgtcc ata
//
LOCUS       DAZGTJ010000070        27501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000070 DAZGTJ010000000
VERSION     DAZGTJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.070"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            69..326
                     /gene="bhsA"
                     /locus_tag="ACHK8E_003487"
                     /gene_synonym="ycfR"
     CDS             69..326
                     /gene="bhsA"
                     /locus_tag="ACHK8E_003487"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP0345703.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(409..1371)
                     /gene="ldtC"
                     /locus_tag="ACHK8E_003488"
     CDS             complement(409..1371)
                     /gene="ldtC"
                     /locus_tag="ACHK8E_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP0345704.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1515..4961)
                     /gene="mfd"
                     /locus_tag="ACHK8E_003489"
     CDS             complement(1515..4961)
                     /gene="mfd"
                     /locus_tag="ACHK8E_003489"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP0345705.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5089..6162)
                     /locus_tag="ACHK8E_003490"
     CDS             complement(5089..6162)
                     /locus_tag="ACHK8E_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP0345706.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6424..7623
                     /gene="lolC"
                     /locus_tag="ACHK8E_003491"
     CDS             6424..7623
                     /gene="lolC"
                     /locus_tag="ACHK8E_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP0345707.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7616..8317
                     /gene="lolD"
                     /locus_tag="ACHK8E_003492"
     CDS             7616..8317
                     /gene="lolD"
                     /locus_tag="ACHK8E_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP0345708.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8317..9561
                     /gene="lolE"
                     /locus_tag="ACHK8E_003493"
     CDS             8317..9561
                     /gene="lolE"
                     /locus_tag="ACHK8E_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP0345709.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9590..10501
                     /gene="nagK"
                     /locus_tag="ACHK8E_003494"
     CDS             9590..10501
                     /gene="nagK"
                     /locus_tag="ACHK8E_003494"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP0345710.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10517..11338
                     /gene="cobB"
                     /locus_tag="ACHK8E_003495"
     CDS             10517..11338
                     /gene="cobB"
                     /locus_tag="ACHK8E_003495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP0345711.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11464..12249)
                     /locus_tag="ACHK8E_003496"
     CDS             complement(11464..12249)
                     /locus_tag="ACHK8E_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP0345712.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12246..12707)
                     /locus_tag="ACHK8E_003497"
     CDS             complement(12246..12707)
                     /locus_tag="ACHK8E_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP0345713.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            complement(12765..13811)
                     /gene="potD"
                     /locus_tag="ACHK8E_003498"
     CDS             complement(12765..13811)
                     /gene="potD"
                     /locus_tag="ACHK8E_003498"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP0345714.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13808..14602)
                     /gene="potC"
                     /locus_tag="ACHK8E_003499"
     CDS             complement(13808..14602)
                     /gene="potC"
                     /locus_tag="ACHK8E_003499"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP0345715.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14599..15456)
                     /gene="potB"
                     /locus_tag="ACHK8E_003500"
     CDS             complement(14599..15456)
                     /gene="potB"
                     /locus_tag="ACHK8E_003500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP0345716.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15440..16576)
                     /gene="potA"
                     /locus_tag="ACHK8E_003501"
     CDS             complement(15440..16576)
                     /gene="potA"
                     /locus_tag="ACHK8E_003501"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP0345717.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16826..18055
                     /gene="pepT"
                     /locus_tag="ACHK8E_003502"
     CDS             16826..18055
                     /gene="pepT"
                     /locus_tag="ACHK8E_003502"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP0345718.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(18201..19322)
                     /gene="roxA"
                     /locus_tag="ACHK8E_003503"
                     /gene_synonym="ycfD"
     CDS             complement(18201..19322)
                     /gene="roxA"
                     /locus_tag="ACHK8E_003503"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP0345719.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19398..20858)
                     /gene="phoQ"
                     /locus_tag="ACHK8E_003504"
     CDS             complement(19398..20858)
                     /gene="phoQ"
                     /locus_tag="ACHK8E_003504"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP0345720.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20858..21529)
                     /gene="phoP"
                     /locus_tag="ACHK8E_003505"
     CDS             complement(20858..21529)
                     /gene="phoP"
                     /locus_tag="ACHK8E_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP0345721.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21698..23068)
                     /gene="purB"
                     /locus_tag="ACHK8E_003506"
     CDS             complement(21698..23068)
                     /gene="purB"
                     /locus_tag="ACHK8E_003506"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP0345722.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(23072..23713)
                     /gene="hflD"
                     /locus_tag="ACHK8E_003507"
     CDS             complement(23072..23713)
                     /gene="hflD"
                     /locus_tag="ACHK8E_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP0345723.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23749..24855)
                     /gene="mnmA"
                     /locus_tag="ACHK8E_003508"
                     /gene_synonym="trmU"
     CDS             complement(23749..24855)
                     /gene="mnmA"
                     /locus_tag="ACHK8E_003508"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP0345724.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            complement(24909..25370)
                     /gene="nudJ"
                     /locus_tag="ACHK8E_003509"
     CDS             complement(24909..25370)
                     /gene="nudJ"
                     /locus_tag="ACHK8E_003509"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP0345725.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25380..26033)
                     /gene="rluE"
                     /locus_tag="ACHK8E_003510"
     CDS             complement(25380..26033)
                     /gene="rluE"
                     /locus_tag="ACHK8E_003510"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP0345726.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            26205..27455
                     /gene="icd"
                     /locus_tag="ACHK8E_003511"
     CDS             26205..27455
                     /gene="icd"
                     /locus_tag="ACHK8E_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP0345727.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6703 a         7009 c         7128 g         6661 t
ORIGIN      
        1 tctatcattt agttatcgat cgttaagtaa ttgcttgcga cgtcattcat ctgcataagg
       61 ccactattat gaaaaacgta aaaaccctca tcgctgcggc gattttaagc tcaatgtcat
      121 ttgccagctt tgcggctgtc gaagttcagt caacgccaga aggccaacaa aaagtcggta
      181 ctatcagtgc taacgcgggg acaaatctgg gatcgctgga agagcagctg gcgcaaaaag
      241 cggatgagat gggcgcaaaa tctttccgta ttacttctgt aaccggtccg aataccctcc
      301 atggaacagc agtaatttat aaataagcat taaccctcat taatgcctgc tactgctgat
      361 ttttttcccc gcgacatgcc gtgtcgcggg gattttttta tccgagcttt acagagtttg
      421 tgggctcact tgatgacggc ggacatccac cggcatcccg gaacggacat ccatcacatg
      481 ttgcatcact tcagcgtcag tttgtgctgc atctttaaat gattgcattg cgctattcag
      541 cgtaattggc agcagctgcg gatcgtcatc aatcttctct gacagcggct gatgtacttc
      601 aaccagacgc gcaccgtttg gttcggcaga gacttttatc ggagtgttga tgatattcac
      661 tttggtgcct ggggtgacct ggctaaagag tgttttgata tcgtcatccc gcagacgaat
      721 acagccagaa cttacccgca tgccaatgcc gaaatcggcg ttcgtaccgt ggagcaaata
      781 aacgccgcca taggccgcca ggcgaatcgc atgatggccc attggattat ccggtccagc
      841 cggaactacc gcaggcaact caattccctg tgctttataa cgggcgcgga tgtttgccgt
      901 tggcgtccag gttgggtttg cacgtttgtc tgaaacggtg gtcaccattg tcggcgtcag
      961 cgtgtcacca cctaactgac caatgccgat gggatacacg gttaccgaat ttttacccgg
     1021 cgggtagtaa tagagacgca gttccgcaat attgatcaca atgccttcgc gcggcgcatc
     1081 tggaagtagg gtttgcaacg ggatcgttaa cacgctgccc gcgcgcggta cgtaaggatc
     1141 aacgccgggg ttagcctgta acagagcgag aaagccgacg ttatattttt tggcgatggc
     1201 ttccagagaa ccaccgtcat tttccaccac atgaaatttg ttttcgccaa ccagacgact
     1261 gcccgctggc ggcagcggcc aggtgttggc ttttgccggt agcgccagcg ccacggcagc
     1321 ggcgaacgta aaaaacgtta gccagcgaga aaaacgcgtt ttgatcatca ccaaaaatcc
     1381 ataataaata taaggttatt gtaataaaca gataagcctg aattatggat ggtgacagtg
     1441 tcggatagtg cagggaagtg caaagaattt gtaaatgttg cagatggggg cgcagaaacg
     1501 cccccgattt accattaagc gatcgcgttc tcttccagtt cacgcataaa ctggcgtacc
     1561 cattcgatac gcgttttccg ctcactcaaa tcctgaataa acttcaggcg cgtcggacca
     1621 tcgaggcgat aatgctgcgg ctgtttttgc agcaaaccaa tcaaccaggc cggattaacg
     1681 tgattcttct cggcaaattc gatcacgccg cctttctcat taccttccag cttcctgatc
     1741 cccagtttct gcgcttgctg gcgcagacgg gcaatatcca gcagggtacg cgccggatcc
     1801 ggcagcaggc cgaagcgatc gataagctcg actttgatct cttccagttc gttttccgtt
     1861 ttggcgctgg caatacgttt gtagaacgac agacgcgtgt tcacgtcagg gatgaaatca
     1921 tcgggcaata gcgacggcat ccgcagctcg acttctgttt gctgactggt gagatcttcc
     1981 agcgacggct cgcgtccggc tttcagcgca tcgacagcgt tttccagcaa ctccatatac
     2041 agcgagaaac cgatggtttc cattgagccg ctttgttctt cgccaagcag ttcacctgcg
     2101 ccgcggatct ccagatcgtg cgttgccagc gcaaaacctg ccccgagatc ttccagcgag
     2161 gcaatcgctt caagacgttt ttgcgcatcg gtagtcatcg cttttggatg cggcgtcagc
     2221 agccatgcat atgcctggtg gtgcgaacgc ccgacgcgac cgcgtaactg gtgcaactgt
     2281 gccagaccga agtgatccgc gcgttcaatg ataatggtgt tggcggtagg aatgtcgatc
     2341 ccggtttcga taatggtggt acaaaccagc acgttgaaac gttgatgatg gaaatcattc
     2401 atcacccgtt ccagttcgcg ctcacgcatt tgcccgtgac cgatggcaat ccgcgcttca
     2461 ggtaccagtt ctgccagccg ttcggcggct ttctggatgt tttccacatc attgtagaga
     2521 taataaacct gtcctccgcg caaaatttca cgcaggatcg cctcccggac caccagactg
     2581 tcatactcac ggacaaaggt tttaactgcc agacgacggg cgggcggcgt ggcgataatc
     2641 gacaggtcac gcattccgct cattgccata ttcagcgtac gtgggatcgg cgttgcagta
     2701 agcgtcagaa tatccacgtt cgcgcgcatc gctttaatgc gctctttatg acgcaccccg
     2761 aagcggtgtt cttcatcgac aatcagcagg cctaaatctt taaacttgac gtcactttgc
     2821 agcagtttgt gcgtaccgat cagaatatcg attttccctt ccgctacttc cgcaaggatt
     2881 tgcgtctgct ctttggcgct gcggaaacgg gagatcattt cgatacgtac cggccagttg
     2941 gcgaaacggt cgcggaagtt gtcgtaatgc tgctgcgcga gaagggtggt tggcaccagc
     3001 accgccacct gcttgtggtt atctactgcc aggaaagcgg cgcgcatcgc cacttctgtt
     3061 ttaccaaagc caacatcgcc gcacaccaga cgatccattg ccagcggctg acacatgtcg
     3121 ctaagtaccg cattaatggc ctgtgcctga tccggcgtgg tttcaaacgg gaagctgtcg
     3181 cagaacaact gatactgctc gcgatcgtgt ttaaacgcga agccctcttt ggcggcgcgt
     3241 tgcgcgtaga tatccagcaa ttctgctgcc acatcacgca ctttttccgc cgctttctgc
     3301 cgcgcgcgtg accaggcatc gccgccaagt ttatgcagcg gggcgttttc ttccgcgcca
     3361 cccgcgtaac ggctaatcag atgcagtgac gacaccggaa catacagttt ggcgtcgttg
     3421 gcataggtga gcatcaggta ctcgccagta ataccgcccg cttccagcgt ggtcattccg
     3481 gcatagcgcc cgacaccgtg ctccagatgg accaccggct gaccaatatg cagctccgcg
     3541 aggttacgga tcagtgtatc ggggttgatg gcgcggcgag aatcctgacg acgacgggca
     3601 acgcgttcac cgagcagatc gctttcgcaa atcagcgcca gattacgtac cgtatcgaca
     3661 aaaccatgtt cggcagcgcc aatcatcaaa taacgcccac ggtcgctggc ttcatcaaga
     3721 cgcataatgc gttgcggagc aattttaatt cgcgcgagca gttcacccag cgcttcacgg
     3781 cgaccttcac tttctaccga gaacaccacc ggaccgtcga aagactcgag gaacttacgc
     3841 agcgcatcca gcggtgcttt ttgttgtgcc tgaacggcca ggtctggcag tttctggaaa
     3901 cctaaattgg cattcgcggc ttttgtcggt aaatgttcag tttttagctg cacacgcggc
     3961 cagtttttca gctctgaaaa gagctcgtct acccgcagcc agagcgattg tggtggcaac
     4021 agtgggcgca tcggatcgac gccgcgattt tcaaaacgcg ccagcgtgtc agcctggaaa
     4081 cgttcggcac tgttttccag atcgccagta ttcaccaaca gcgtattggc agggaaataa
     4141 ctgaacagtg gcggcagcgg ttcgctgaag aatagcggct gccagtactc gatcccggca
     4201 ggtaatgtgc ctttactcac ttgctgataa atatgttccg gatcgcgctt cacttcgaag
     4261 gtatcgcgcc actggctgcg aaacagttca attgccgctt tatcggtcgg aaattcgtgc
     4321 gcgggcagca gattgatctc ttctacttcc tccagcgtgc gctggctatc gacgtcaaac
     4381 actcgcaggc tgtcgatttc atcatcaaag aaatcaagac gataaggcag ctcactgccc
     4441 atcgggaaga gatccagcaa cgcgccgcgc gtggcgtatt cgccgtgctc cattacctgg
     4501 tcaacatggc gataaccggc gctgtccagt tgggttcgta atgcatctcg tgacaggcgc
     4561 tgaccttttt tcatcaccag cgcatgaccg tggagaaaac tgtgcgggca aacgcgctgc
     4621 ataagcgtat tcaccggaac aatcagtacg ccacgctgca tggtcggtag ctggtaaagg
     4681 gtggaaaggc gcgaggagat aatgtcctga tgaggcgaaa aactgtcgta gggaagggtt
     4741 tcccagtccg ccagattcat caccatctga tcggtaaact ggctgatttc atcatgcaaa
     4801 cgcagagcat tttgcatatc tggtgcaata agtaccaccg gaccggcgtg acgttcggca
     4861 atttccgcta ccagcgtcgc gcaggcagcg ccggttaact cgcccagcag acgctgctca
     4921 cctgctttga cgggcagcgt ataacgatat tgttcaggca taacggttgt cagattctcg
     4981 ttaggatatg cctcaacata tgggggcata tctctgataa gcaatgctgt tcattatccg
     5041 taatcgacat ggtttagcaa attgaatcgc cccggctggg gcgatggctt agcgggagta
     5101 actgacggca gcgggcagtg aaggcgggga gaacaacaga tcgctgaatg cacgctgtga
     5161 aagttttctt accagcaagc ccgcaacagt acagatgaac aaactgacaa agggataaac
     5221 catcaggagt gtcagttcga cttgcggcga ccagcgtgcc gcgttcattg gcgcaagcag
     5281 agttaagctg aatatctcta ccagaatgcg atgggtggtg tagatagcaa tggtgttggt
     5341 gccaatcaca ttcagcaggc tggtggagcg cataccgaaa cgttgctcgt attgataaaa
     5401 cagcttcatg atcaccacaa tcgataccag cgacaacagc agcgagatag taaacaacca
     5461 ggcaccgacc gccagaacgg tcagcaaaga agtcatcagc agatggcggc gcaacggcac
     5521 ctctttaacg taggtcatta atgttgcgcc aaaccatgcg ccaaggctgt aataaggcaa
     5581 attgcggatc acactgttca ttccccacca cggcgtggga acgaaattaa ccgccacact
     5641 catcagtaca aacaaggcga atagtggcag cgccagacgg ctaaaaattt tacataccac
     5701 gaaataaaca attaacgcat acagatacca caaactggtg ctggcggtga tcatcccgtg
     5761 caggaactca ccggtggaat cggcatacgc ggcattggag gcattgctta aatcgcgctc
     5821 aggtgccagc cactggttca gcgcacttaa cgccagccac tgcaccacgc cccaaagcgc
     5881 cagcacccag aagatgttcc agatccgttt atcgagacaa tttccccacg gtacgctgtc
     5941 gatataacgg cgaatcaaat agccggatat aaagaaaaaa accggcatac gaaagggtgc
     6001 aaggtaaaga ttgaaataga tccagcattt gctcaggact tccgataacg gatgctgaaa
     6061 agtggtgaga tgcggataaa aggtaatgac cgagtgataa atcaccacca gacaaataca
     6121 taaccctttg atctggttaa tccatagctc tttttgtttc atcaggacaa cacgctcttt
     6181 ttatttaaca tgctgttgat cctgaggggc cagtcgcgtg gtgtaatgcg tatctgtctg
     6241 tatttggtta tttttcagat aaagatcagg ctacaaggcc gatgagaaaa tgaggccctt
     6301 tagccgattg atttgctggc acttttcaga ttagccctga cgatcactta cagttcagac
     6361 gtttacccat cccgctttcg cttatatact cgtgtctttg ctaacagcaa ccagacggat
     6421 ttcatgtacc aacctgtcgc tctatttatt ggcctgcgtt acatgcgtgg gcgtgcagcg
     6481 gatcgcttcg gtcgtttcgt ctcctggctt tctaccatcg gcattaccct cggggtgatg
     6541 gcgctggtca cagtattgtc agtgatgaac ggctttgagc gcgagctaca aaacaacatc
     6601 cttggcctga tgccacaggc aattctctct tctgagcatg gctctcttaa cccgcagcaa
     6661 ctcccggaaa cggcagtcaa actggacggc gttaatcgcg tcgcacctat tactaccggt
     6721 gatgtggtac tgcaaagcgc gcgcagcgtg gcggtcgggg tgatgctggg tatcgacccg
     6781 gcacaaaaag atccactaac gccgtatctg gtcaatgtga aacaaactga cctcgagccg
     6841 gggaaatata atgtcatcct cggtgaacag ctggcctcac agttgggcgt caatcgcggt
     6901 gatcaaatcc gcgtgatggt gccatctgcc agccagttca cgccgatggg gcgtattcca
     6961 agccagcgcc tgttcaatgt gattggcact ttcgccgcca acagtgaagt cgatggctat
     7021 gaaatgctgg tgaatattga ggatgcctcg cgtctgatgc gttatccggc aggcaatatt
     7081 accggctggc gtttgtggct ggatgagccg ctgaaagtcg actcattaag tcaacaaaaa
     7141 ctgcctgaag gcagcaaatg gcaggactgg cgtgaccgta aaggcgagct gttccaggcc
     7201 gtacgcatgg aaaaaaatat gatgggcttg ctgctgagcc tgattgttgc cgtagcggcg
     7261 tttaacatta ttacctcgct agggctaatg gtgatggaga agcagggcga agtcgcgatc
     7321 ctgcaaacgc aaggcttaac tccgcgacaa atcatgatgg tctttatggt gcaaggggcc
     7381 agcgccggga ttatcggtgc gatcctcgga gcggcgcttg gcgcactgct tgccagccag
     7441 ttaaataatc tgatgccgat aatcggcgtc ctgcttgatg gcgcggcgct gccggtcgct
     7501 atcgaacctt tacaggtcat tgttattgcg ctggtggcga tggctatcgc gctgctgtct
     7561 acgctttacc cttcatggcg cgctgccgcc acacaacccg ctgaggcttt acgttatgaa
     7621 taagatcctg ttgcaatgcg acaacctgtg caaacgctat caggaaggca gtgtgcaaac
     7681 cgatgtgctg cacaatgtca gtttcagcgt gggcgaaggt gaaatgatgg caattgtcgg
     7741 tagctctggt tccggtaaaa gtaccttgct gcacctgtta ggcgggctgg atacgccaac
     7801 ctccggcgat gtgatcttta acggtcagcc gatgagcaaa ctgtcgtcgg cggcgaaagc
     7861 agaactgcgc aaccagaagc tgggctttat ttatcagttt caccacctgc tgccggattt
     7921 tactgccctg gaaaacgtgg ctatgccgct gctgattggc aagaaaaagc ccgctgaaat
     7981 caacagccgt gcgctggaga tgttaaaagc ggtggggctg gagcatcgtg cgaatcaccg
     8041 cccatctgaa ctttctggcg gcgaacgcca gcgcgtggcg attgcccgtg cgctggtcaa
     8101 taacccgcgg ctggtgctgg cggatgaacc tactggtaac ctcgatgcac gtaacgccga
     8161 cagcatcttc cagttgcttg gagaattgaa tcgcttgcag ggcaccgcct tcctggtggt
     8221 tactcacgac ctgcaactgg caaaacgtat gagccgccaa ctggagatgc gtgatggtcg
     8281 tctgacggcg gaactgagcc tgatgggggc ggagtaatgg cgatgccttt atcgttattg
     8341 attggcctgc gttttagccg cggacggcgg cgcggcggca tggtgtcgct gatctccgtc
     8401 atttctacca ttggcattgc ccttggcgtg gcggtattga tcgtcggctt aagcgcgatg
     8461 aacggctttg aacgcgaact gaataaccgc attctggcgg tggtgccgca tggcgaaata
     8521 gaggcggtgg atcaaccgtg gactaactgg caggaagccc tggataacgt gcaaaaagtg
     8581 ccaggtattg ccgccgctgc gccgtatatc aatttcaccg gactggtaga aagtggtgcc
     8641 aatctgcgcg caatccaggt gaagggcgtt aacccgcaac aggaacagcg tctgagcgca
     8701 ttaccctcgt ttgttcaggg ggatgcctgg cgcaatttta aagcgggcga acagcaaatt
     8761 atcatcggca aaggcgtggc ggatgcgctg aaagtgaagc agggcgattg ggtgtcgatt
     8821 atgatcccca actcgaatcc cgagcataaa ctgatgcagc caaaacgtgt gcgtttgcac
     8881 attgccggta ttttgcagtt gagtggtcaa ctcgatcaca gttttgccat gatcccgctg
     8941 gcggatgccc agcaatatct tgatatgggt tccagcgtgt caggtattgc ccttaaaatg
     9001 acggatgttt tcaacgccaa taagctggta cgcgatgcgg gtgaagtgac caacagctat
     9061 gtttatatta aaagctggat tggtacttac ggctatatgt atcgcgatat ccagatgatc
     9121 cgcgccatta tgtatctggc gatggtactg gtgattggcg tggcctgttt caacatcgtc
     9181 tccaccttag tgatggcggt gaaagacaag agtggcgata tcgcagtatt aagaacgctg
     9241 ggggcgaaag atggtttaat tcgcgccatc tttgtctggt atggattgct ggcagggctg
     9301 ttcggtagcc tgtgtggtgt gattatcggc gtagttgttt cactgcaact taccccgatt
     9361 attgagtgga ttgaaaagtt gatcggtcat cagttcctct ccagcgatat ctattttatt
     9421 gacttcctgc catcggaatt gcactggctg gacgtcttct acgtactggt cacagcattg
     9481 ttgctgagtc ttttggcaag ttggtatccg gcgcgacgcg ccagtaatat tgaccctgcg
     9541 cgagtcctta gcggccagta aaggcagtac attaaaacaa ggagcggcaa tgtattacgg
     9601 gtttgatatt ggtggaacaa aaattgcgct tggcgtgttt gatagcggtc ggcagttgca
     9661 gtgggaaaag cgggtgccga caccgcgtga cagctatgac gcatttttag atgcagtgtg
     9721 cgagctggta gccgaagctg accggcgttt tggctgtaaa ggttctgtcg gcatcggtat
     9781 tccgggaatg ccggaaacag aagatggtac gctgtatgcc gccaatgtcc ctgctgccag
     9841 cggtaaaccg ctgcgtgccg acctgagcgc acgtcttgat cgcgatgtac gccttgataa
     9901 cgatgccaac tgttttgccc tttctgaagc atgggatgat gaatttactc aatatccact
     9961 ggtgatgggg ttgattctcg gcaccggcgt tggcggcggg ctgattttca acggtaaacc
    10021 cattaccggt aaaagctata ttaccggcga gtttggccat atgcgtctgc cggttgatgc
    10081 gttaaccatg atggggctgg atttcccgtt acgccgctgc ggctgtggtc agcatggctg
    10141 cattgaaaat tatctgtctg gtcgcggttt tgcgtggctg tatcaacact attatcatca
    10201 accgttgcag gctcccgaaa ttattgcgct ttatgatcaa ggtgatgagc aggcaagggc
    10261 gcacgttgag cgttatctgg atttattagc ggtttgtctg ggaaatatcc tgaccattgt
    10321 tgaccctgac ctggtcgtca ttggtggtgg cttatcgaat ttcccggcaa tcacaacgca
    10381 actggcggac aggctgcctc gtcatctctt acctgtagct cgtgttccgc gcattgaacg
    10441 cgcgcgccac ggtgatgcgg gaggaatgcg tggtgcggcc ttcctacatc taaccgatta
    10501 aacaacagag gttgctatgc tgtcgcgtcg gggtcatcgg ttaagtcgtt ttcgtaaaaa
    10561 taaacgccgt ctgcgcgagc gtttgcgtca gcgtattttt ttcagagata aagtggtgcc
    10621 ggaagcaatg gaaaaaccaa gagtactcgt actgacaggg gcagggattt ctgcggaatc
    10681 aggtattcgt acctttcgcg ccgcagatgg cctgtgggaa gaacatcggg ttgaagatgt
    10741 ggcaacgccg gaaggtttcg atcgcgatcc tgaactggtg caaacgtttt ataacgcccg
    10801 tcgtcgacag ctgcagcagc cagaaattca gcctaacgcc gcgcatcttg cgctggcgaa
    10861 actgcaagac gccctcggcg atcgcttttt gctggtgacg cagaatatcg acaacctgca
    10921 tgaacgcgca ggtaatacca atgtcattca tatgcatggg gaactgctga aagttcgctg
    10981 ttcgcaaagt ggtcaggttc tcgactggac cggagatgtt accccagaag ataaatgcca
    11041 ttgttgccag ttcccggcac ccttgcgccc gcacgtagtg tggtttggcg aaatgccact
    11101 cggcatggat gaaatttata tggcgttgtc gatggccgat attttcattg ctattggtac
    11161 ttcggggcat gtttatccgg cggctgggtt tgttcacgaa gcgaaactgc atggcgcgca
    11221 caccgtggaa ctgaatcttg aaccgagtca ggttggtaat gaatttgccg agaaatatta
    11281 cggtccggca agccaggtgg tgccagagtt tgttgaaaag ttgctgaagg gattataaag
    11341 cgggaaaaat tgcctgatgc gctacgcttg tcaggcctgt gaaggggcgc aattaattga
    11401 atttgcgggt tttgtgggcc ggataaggcg ttcgcgccgc atccggcaac tttactgcct
    11461 cttttaccgt ttactcgtcc atagtaacgc ggcaataaac gtacagacac caatcagccc
    11521 tgcccagagc ggcgatattc tggtaatcgc aaagctgtta tcgatataca aacataaagc
    11581 ctgatagccc atattggcaa cagccatagc cactgcaaat tttaacgcat aataaaggcg
    11641 cgttgtgtag ttaaaaaatg cagcaaagac gatgaatgca actgcccagg caaaaaggcc
    11701 taatgtcgtg gtactgccgg gaagatcatt ctcctgctgt tgtccgggat gcggataata
    11761 tgtcagtggc ggcgtcggtt gttttaatat ctgtgcttgt aaaacagcca atgcattatt
    11821 gattccctga taccagagat ctgcatgtaa ataagccaga gtgtgatttt ctaccagaag
    11881 gccgagatgt ttatcgtcca tgctgctttc aacctggccg ttggttatca taattaaccg
    11941 tggctcgcgg gtagtaaaaa gtaacagaac acttcctttt acattactca ttgaagatgc
    12001 cgcatgcgcc agcatcgctt tgccattttg cctgaagctt gcttcattaa cgccaggtac
    12061 aaccacgata aacggcgcaa tcccggtttg ttgacgaaaa agttcggctt tacgcgcgat
    12121 ctcttcggca tccggtacag taccggtaac atccgtaaaa agagtcttca ttattggaat
    12181 caatggctta ctggttaccg tcagaggcag gagtatcagt aaactgagca atattatgat
    12241 ttttttcatg ggcgggactt ccgtttcctg cgtgtctttt tagagtaggg catactgatt
    12301 aataaaccga cggagataaa aataacgccc ataatgccga taaaccccca tttaaaaaaa
    12361 ttaacccacg ggttatttat ctcagccttt tcaggatgtc ctggagaata ataaacatta
    12421 actttatcgc cttcactgtc ttcataaaaa tcgctgccaa tacttgaatt gaaaattagc
    12481 gtgtaatctg gcgtcggacg aaaggcaacg acgggatacc acgaaccatt tttacccgtg
    12541 cgatgagaat gactggagtg ccagacggta tcaacaatga cgccttcagt atgaatcgcg
    12601 tctttgctat aggtgaactg gttataaaag ataacaacag aaatgaggat cagtaccaga
    12661 ccgataccga agaatatacg aaagaaaact ctactgtcct ggctcattag ccacatcctt
    12721 gctaaataaa aaacgggcgg taataccacc gcccgcttgc tgaattaacg tcctgctttc
    12781 agcttctgat aatactcttc ataaatgctg ctggctgcgc caacgtcatt ctgccattca
    12841 ccatttttaa tggtttcagc atccgggtag agcgttttat cgttcgccac ttccggactt
    12901 aacagcttac gcgccgcaag gtttggcgtt ggataaccga tagtttcagc aacctgtttt
    12961 gccacatccg ggcgcagcag gaagttgatc aatttcagtg cgccttcttt gtttttggca
    13021 tttgccggga tcgccaggct gtccatccag aaaatgccgc cttctttcgg ccacaccaca
    13081 tcaattggcg tacccgcctg gcgtgcaacg aaagcagaac cgttccagat catgccgagg
    13141 ttaacttcgc cttccatata cgggttagcg gggttatcgg agttaaacgc cgccacgttt
    13201 ggcatcagtt ttttcagctc gttgtatgca gcttcaatct ctttcggatc ggtggtgtta
    13261 ccggagtagc ccagcttacg cagcgccatc tggaacactt cacgggcatc gtcggtcagc
    13321 agcaggctgc ctttgtactc aggcttccac agatcggccc agctggtgac agatttcgga
    13381 tccaccgcat caccgttaac gccaatcgcc gttgcacccc agatatacgg aatggaatag
    13441 tcgttattcg ggtcaaacgg cttgttgagc atgtctggat cgaggttgct gaaattgctt
    13501 aacttcgact tgtcgatctt ctggatcatc ccttctttac gcattttatc gacgtaatag
    13561 gttgaaggaa ccaccaggtc ataggcaccg tctttgtacg ttttcagctt cgcgtacatg
    13621 gtttcgttcg actcgtaagt cgaatagata accttaatac cggtttcttt ggtgaactgt
    13681 tcaagcagtc ctggcggcac gtactcggtc cagttgtaga aatacagcgt gttgttgtca
    13741 tcggcgtgag cggcgctcat gcccagtgcc agagcacccg ccgcgagcag gtggcgtgac
    13801 cattttttca tttaacgtcc cctgtgttac ctttcgtttt atcacgagca ataagctggc
    13861 tggcaattac catcaccagc gacagcacca gtaatatggt tgccagcgcg ttaacttccg
    13921 gcgatacgcc gactttgacc atcgaataaa tttttaacgg cagaatttca taactcggcc
    13981 cggtgacaaa cgaagaaacc accacgtcgt ccatcgacag ggtaaagctt aacacccagc
    14041 ccgccgctac cgctggcatt gccagaggca gaatgatttt tcgcagaatg gtaaattcgc
    14101 tggcaccgag atctttcgcc gcttccagca ttcgcacgtc aaaacctttc aggcgcgaat
    14161 acactgtcac caccacaaat ggcaggcaga aggtgatatg cgagaacagc agcgaccaga
    14221 agccaagctg aatacccagc agcataaaca gcaccagcag cgaaatcgcc atcacgatat
    14281 ctggtgacat catcaccaca aacagcattc cgctaacgaa cggcttacca cgaaagcgat
    14341 aacggtacag cgcaactgct gtcagtgaac cgataagcgt agcaaacgtc gccgaaaaca
    14401 ccgccattgt tagtgaatgc tgcgctgcct gtaacaggct gtcgttgttc atcagcaggc
    14461 tataccattt ggtggtaaaa ccctgccagt tgatgccaaa gcgcgagctg ttaaaggagt
    14521 tcacaatcaa aataatgatt gggatataca ggtacgcgta gatagcggtc ataaaaccgc
    14581 cgcgaagcag tcgaccgatc attcgagttc caccttctta ttcagcagac gagaagcgcg
    14641 ccagtaaacc agcaacatca ggcccattac gatagtcagc gtaatgctgg tggctgcacc
    14701 aaacggccag tcacgaatat tgaggaactg taccttgatg acgttaccga tcagcaggtt
    14761 tttcgcaccg cccatcaggt cggacacata gaacaggccc atcgctggca gcatcaccag
    14821 cagacatccg gcaataattc ccggcatcgt tagtgggata atgatgcgga taaaggtctg
    14881 caacttgctg gcaccgagat cgcgcgccgc ctcaagcaac ggcttatcca gtttttcgat
    14941 actggagtac agcggcatca ccataaacgg cagcagaatg taaaccagac cgataatcac
    15001 cgcactaggc gtgaacatga tgcgaattgg tgtatcgata acgcccagcc agagcaaaaa
    15061 ctcgttgaga tagcctttgg tgctgaggaa aattttcagc ccgtaaatac gaatcaatga
    15121 gttggtccag aacggaacaa tcagcagaaa cagcagcagc ggacgcacct tgtgtggcag
    15181 cttcgccaga aaccaggcaa aaggatagcc cagcaccagg caggcgaggg tggcgatcag
    15241 cgccatattc agcgagtgca atagcacttc aaaatagagc ggatcgagca gacgcgtgta
    15301 gttatccagc gtaaagacca ttttgacgaa actggcgtcg tcgcgggtca aaaagctggt
    15361 gccaatgatc atcaggttgg gcagaaagac aaacaacaca agccaaccga caatagtgac
    15421 aatcactaca ttctggaact tacttgtgtt cttcatcagc cagtacgacc tcccagcttt
    15481 ctacccaatt aatggccatt ttttggtcga gagagtggtc aaagtcagga tcgtcttcat
    15541 tgaagaattc gctgaccatc accatcttgc cattttccag ctcaacaacc gactccagcg
    15601 tcatgccttt gtagttacgc tcgcgaacgt aaccaatcag cccttcggcg tggttgtcgt
    15661 cgttaatctc ttcaacacgt aagtcttccg ggcgcagcag aacatgcagt ttttgccccg
    15721 gttcaacggt gaagttaacg tagatattac attcgcggcc ttcaacgttg gcgcgtacgc
    15781 gctgctcgtc gagacgttcg atgacagtgg cgttaaacat attgatttcg ccaataaacc
    15841 cggcaacaaa caggtttttc ggctcttcgt agatttcacg cggcgtgccg tcttgctcaa
    15901 tgcgaccatc gcgcatcacc acgatcctgt ccgacatggt aagtgcttct tcctggtcgt
    15961 gagttacgaa cacgaatgta ataccaagct tacgctgtaa cgctttcagc tcgttctgca
    16021 tttgcttgcg cagtttgtaa tccagcgctg agagcgactc atccagcaac aacagacgag
    16081 gcttgttaac caccgcgcgg gcaatggcga cgcgttgctg ttgaccacca gagagctgat
    16141 gcggtttgcg ttgagcgaag gtttccagct gcaccatccg cagggcttcc atcacgcggg
    16201 gcgtaatttc atcagcgggg gttttttgca tgcgcaaccc aaaggccaca ttttcgaaca
    16261 cggtcatgtg ggggaaaagt gcgtagcttt ggaaaacagt gttcacgtag cggttttccg
    16321 ccggaacgtg ggtgatgtcc tcgttatcca acatgatgcg tccggaatca acagtttcca
    16381 gacctgcaat caggcgaaga acggttgttt taccgcagcc agaagggcca agcagcgtga
    16441 ggaactcgcc attgttgata gtcagatcca gctggggaat gacctcttta ccatcaaagc
    16501 atttgcgaat tcccgccaat tgcaccagcg gtgaaagcga actcggttgt ttattcaatt
    16561 ttttactctg tcccatgtaa acgcaacgga tggcttaccg atgcggggtt tgtggttaac
    16621 caccttggtg actcttaatg agggcggtaa ttctacggca aaccgcttga atcgccaatc
    16681 tttgttgtga attactggct tagctttata ttcattaagg taatgctgat aaatattccc
    16741 gcttgcaggg ctaaaagtga cctgacgcaa tatttgtctt ttcttgcttc ttaataatgt
    16801 tgtcacaaaa agtgagggtg actacatgga taaactactt gagcgatttt tgaactacgt
    16861 gtctctggat acccaatcaa aggcaggggt gagacaggtt cccagcacgg aaggccaatg
    16921 gaagttattg catctgctga aagagcagct cgaagagatg gggcttatca atgtgacctt
    16981 aagtgagaag ggcactttga tggcgacgtt accggctaac gtccctggcg atatcccggc
    17041 gattggcttt atttctcatg tggatacctc accggattgc agcggcaaaa atgtgaatcc
    17101 gcaaattgtt gaaaactatc gcggtggcga tattgcgctg ggtatcggcg atgaagtttt
    17161 atcaccggtt atgttcccgg tgctgcatca gctactgggt cagacgctga ttaccaccga
    17221 tggtaaaacc ttgttaggtg ccgatgacaa agcaggtatt gcagaaatca tgaccgcgct
    17281 ggcggtattg caacaaaaaa acattccgca tggtgatatt cgcgtcgcct ttaccccgga
    17341 tgaagaagtg ggcaaagggg cgaagcattt tgatgttgac gccttcgatg cccgctgggc
    17401 ttacaccgtt gatggtggtg gcgtaggcga actggagttt gaaaacttca acgccgcgtc
    17461 ggtcaatatc aaaattgtcg gtaacaatgt tcacccgggc acggcgaaag gagtaatggt
    17521 aaatgcgctg tcgctggcgg cacgtattca tgcggaagtt ccggcggatg aaagcccgga
    17581 aatgacagaa ggctatgaag gtttctatca cctggcgagc atgaaaggca ccgttgaacg
    17641 ggccgatatg cactacatca tccgtgattt cgaccgtaaa cagtttgaag cgcgtaaacg
    17701 taaaatgatg gagatcgcca aaaaagtggg caaagggtta catcctgatt gctacattga
    17761 actggtgatt gaagacagtt actacaatat gcgcgagaaa gtggttgagc atccgcatat
    17821 tctcgatatc gcccagcagg cgatgcgtga ctgcgatatt gaaccggaac tgaaaccgat
    17881 ccgcggcggt accgacggcg cgcagttgtc gtttatggga ttaccgtgcc cgaacctgtt
    17941 cactggcggt tacaactatc atggtaagca tgagtttgtg actctggaag gtatggaaaa
    18001 agcggtgcag gtgatcgtcc gtattgccga gttgacggcg caacggaagt cgtgattgcc
    18061 cgatgcgtat gtggactgcc aattaccggg atgaaacctt tatattgaca ctggtccaca
    18121 tacttatcgt gcctacaggc gttcagtgca tgtaggcacg ataaggcgca atcagcgccg
    18181 catccggctt aaatccaaac ttacccttcg aagaaccagt atccgctatt gaccagcgcc
    18241 gcgagcatcg cgaggaatga cggatcttcc agcgcatcgc caaaattctc cgcggtcagc
    18301 gcaatgttgc tggcgagcgc atccagtgcc ggacggtgcg gggaatcgat cttctcacca
    18361 ttggcataca cgtcgtcgcc aatgcgcaat acgcgcagac cccccaggcg caccagcaca
    18421 tcaccttgtt tcagcgcatc gtagatttca tccggctgat aaggcggctc tggcggcgcg
    18481 atatccagtt catgacgtga ctgggatata aactcgccaa accattgctt aaaatgttcc
    18541 ggctggttga tcaattcgag catcatctca cgcagtttat ccatctcttg cggcagaacg
    18601 tccgcaggat gagcgcgagg tggaacatcc ggatcgctgt agtagttgcc gcccagttca
    18661 cgttgcagca cataatcggc aaatccacta atcagttccc gcgtatttgg cgcgcgaaaa
    18721 cccacggaat agttcatcgc attttccagc gcgtagcctt catgcgggaa tcctggcgga
    18781 atataaagaa tatcaccagg ctccagctct tcatcgatga tggcttcgaa cggatcgacc
    18841 tgtaacagat ccggatgtgg gcagtgctgt ttcatttgca gcttttcgcc cactcgccag
    18901 cgacgacgtc cggtaccctg aatgataaac acgtcgtact gatcgagatg cgggccgacg
    18961 ccgccgccag gtacagaaaa agagatcatc aggtcatcaa tacgccagtc cggcagttca
    19021 cggaacggtc gcatcagcgc ggcggtcggc tcatgccagt ggttcactgc ttgcactaac
    19081 aatgaccagc tagtttcacc gagatgatcg tagctttcga atggaccgtg gctgacctgc
    19141 catttgccat cctgatgact gaccagtcga ctgtcgactt cgctttccat cgccagaccc
    19201 gccaactcgt ctggagagat cgggtcaata aaattattaa agccgcgttt taacaccacc
    19261 gggcgtttct gccagtgacg ttcaagaaaa tcgggccagt taagagtgag ttggtattcc
    19321 atgttaagca tccacaggct ggtatctgca accgattata acggatgctt aacgtaatgc
    19381 gtgaagtatg gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    19441 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    19501 gtgatttcgc gggctaccgc cagccccaca ccttgcccag ggcgtaaagt atcaacccgt
    19561 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    19621 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    19681 tatttacagg cattatccag cacattgccc atcacctcga caaaatcgtt ctgctcaccg
    19741 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    19801 ttgttcagcg ccgaggtgag attgtccagc agtggagcga ccggatgcag ctcgcggctg
    19861 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    19921 cggctgattt gctccagcat taccggttca gcatcactga cgctcatctt ttcactacgc
    19981 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    20041 gtgagcgtcg tacgatattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    20101 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    20161 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    20221 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    20281 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtc
    20341 aactttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    20401 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    20461 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    20521 cagtcaggct ggatcatctt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    20581 ccgttctcat cataaattag tgtcatcgtg gggctttgct tgtcgatatt ttcgggtaac
    20641 tcgacatgca acttattgtt ttcccacttc gcaagggtat agaacagatt gctctcgcca
    20701 cgtaacagcc gaaacgtggt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    20761 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    20821 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacagatagc
    20881 cctggccgcg aacggtggta atcacttctt gcggatattg tgcctgaatt tttttgcgca
    20941 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    21001 ggagcattaa cgaatctttg ctcaccactt tgccattatt gcgtatcaac gtttccataa
    21061 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    21121 agagatcaac ctgaaacggt ggtagcgaaa tgacctgtga agccagaccg ctattacgcc
    21181 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    21241 catcagcgcc agcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    21301 ccagaatcgg cagtgaaaca tcattgctac gccagcggcg aatcagtgac agaccgtcct
    21361 cgtcaggcaa accgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    21421 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    21481 taagatggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    21541 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgg ttatgggggt aaacattaaa
    21601 taaaccagcg ggaaggggag gtaaagtgaa aaaacaaaaa gtggataatc ttaataagca
    21661 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatgg
    21721 tgatggcgcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    21781 ctggcaacgc cagaccgtcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    21841 gagtcagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    21901 ggattggctc agccagcact tcccagttgt gatccagttc atccagcaga tggtcacggt
    21961 tcacttccag tttgctcacc cctttcaggg tggattgata cgcaatcagc gcataaccga
    22021 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    22081 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    22141 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    22201 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    22261 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ctcggcaatg tagtcgtgcg
    22321 gttcgatctg ggtagtgtac gggttccact gaatacccag cgaggtgacg aactcttcgc
    22381 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgactg
    22441 cgccgttgat tttaccgagg atctccacct gattaagctg gcggtactgg cgctccatac
    22501 ggtaggcgac gttagccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    22561 tacgggacag cagcgggata tcgcgatact gaacggcgag atctttaatg ccatcaatca
    22621 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatc aacgcgtggg
    22681 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcatgca
    22741 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    22801 tagtggtacg ctcgatggtt ttgatgcgtg ctgcatcttc ttcgctgaaa ctggcgacaa
    22861 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    22921 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    22981 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    23041 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    23101 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgcaga cgtccgccgc
    23161 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggtagcg cgaactttcg
    23221 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    23281 gcgggctaat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    23341 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    23401 cacctttcgc tgaggagagt ttgcgctcaa gcaccatcag gctgagtgtg tagcgggtta
    23461 actcagcgtt taagccctgg cgactgctgg cattgagcac gccgagcaag gtttccagcc
    23521 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    23581 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    23641 gttgttgcac caggcgtgcc gactgacaaa taccggccag agcgagggtg atgtcatagt
    23701 aattctttgc cacgttcact gcttccttgt tttaagtaaa gaaaataatc agaccggcag
    23761 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgtcat agaatacggc
    23821 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcacttcaa tgcgatcatc
    23881 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    23941 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc aactgctggg caatcagacc
    24001 gacagacatc agacgcgggt gttcatggcc ctgcgcaaca atcagaatgt tgttttcgac
    24061 gtctttatcg accacatacc acggttcttc ggtgccttct ttggtgccac caatacccag
    24121 acctttacgc tgaccgaggg tgtgatacat cagcccctgg tgttcaccaa tttcatcgcc
    24181 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    24241 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    24301 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgccgaccg ggaacaggct
    24361 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    24421 cagaccacgc agcaggcggc tcttgccatc gacatctgcc cgacgtacgt aatgaccggt
    24481 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    24541 ctctttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    24601 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    24661 gagcttgtcg cagacagcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    24721 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    24781 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    24841 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    24901 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    24961 ggatagcgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgcaga
    25021 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    25081 tgaggctggg tcgggcatat ttgctcaagc tcaatagcaa acaggaaacg caaaaacggc
    25141 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    25201 ccggtttctt cccacagctc acgcgcagca gcttccacta gcgtctcatc cgcttccaga
    25261 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    25321 ttgccttctg cgtgcaccac gcaggcaacg gtaacgtgcg gtttaaacat tttgcgtcct
    25381 taatctgtcg cttctcgcca ttcgccattg gcaagattat ccaaagagta atcacccatc
    25441 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    25501 cgatttcgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    25561 cgaattggcg gattacgggg ccataaccat tccggctcct caaccatctc tgcacctgcg
    25621 ggcagggtag ggccatcatt taaggttacg ccattgcgca acgcttcaag ggcgtcttcc
    25681 gtgggaatac cttctacctg tacataatag attttcccgg tacgtttacc cggctgggtt
    25741 aaacgcgcct gcaacgcgcc gttattggtc agcaccagta acccttcgct atcgcggtca
    25801 aggcgacctg ctgcataaac gccttgaacc gggatgaatt cttttaatgt tttgcgtccg
    25861 gcttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaacaggat cacacgcgtg
    25921 ggctggtttt caggtttacg cctggtagaa cgttgcgagc tgaatcgctt aacctggtga
    25981 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    26041 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag ccaattacaa
    26101 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    26161 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    26221 ttgttccggc acaaggcaag aagatcaccc tgcaaaacag caaactcaac gttcctgaaa
    26281 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    26341 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    26401 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    26461 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    26521 cggttggtgg cggtattcgc tctctgaacg ttgccctgcg ccaggaactg gatctctaca
    26581 tctgcctgcg tccggtacgt tactaccagg gcaccccaag cccggttaaa caccctgaac
    26641 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    26701 aagcagactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    26761 agaaaattcg tttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    26821 ccaaacgtct ggtgcgtgca gcgatcgaat acgcaattgc gaacgatcgt gactctgtga
    26881 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gattggggct
    26941 accagctggc gcgtgaagag tttggcggtg aactgatcga cggcggcccg tggctgaaag
    27001 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga tgtgattgct gatgcattcc
    27061 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    27121 gtgactacat ttctgacgct ctggctgcac aggtcggcgg tatcggtatc gccccaggcg
    27181 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatatg
    27241 ccggtcagga caaagttaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    27301 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcaatcaacg
    27361 ccaagactgt aacctatgac ttcgaacgtc tgatggaagg cgctaaactg ctgaaatgca
    27421 gcgagtttgg tgatgcgatc atcaagaaca tgtaatctct acatgtgtta aatattgaaa
    27481 cgggcgtata acacgcccgt t
//
LOCUS       DAZGTJ010000071        26780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000071 DAZGTJ010000000
VERSION     DAZGTJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.071"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHK8E_003512"
     CDS             complement(<1..191)
                     /locus_tag="ACHK8E_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345728.1"
                     /translation="MDIVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGAVT
                     VKNGIWQAIAGGVGNKTNIA"
     gene            complement(227..439)
                     /locus_tag="ACHK8E_003513"
     CDS             complement(227..439)
                     /locus_tag="ACHK8E_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0345729.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEGDTSPTE"
     gene            complement(399..1025)
                     /locus_tag="ACHK8E_003514"
     CDS             complement(399..1025)
                     /locus_tag="ACHK8E_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFP0345730.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            complement(1022..1378)
                     /locus_tag="ACHK8E_003515"
     CDS             complement(1022..1378)
                     /locus_tag="ACHK8E_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP0345731.1"
                     /translation="MIQSRGRPVTGSEIAEKFGVSLCEFNKVARGLTKGSKVVKIKAS
                     EPFTTDTGIVDRFFSLESNPRRNTPRSRNAVPPFSRRSREHAAKNCREEYVQKAERRR
                     RLIKAGLYIDEFENAL"
     gene            complement(1509..2180)
                     /locus_tag="ACHK8E_003516"
     CDS             complement(1509..2180)
                     /locus_tag="ACHK8E_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP0345732.1"
                     /translation="MTTQISVETISPITHNQIPVITTELLAQLYGTETNNIKVNYTRN
                     AERFVCGKHYFKLEGAGLREFKNKVTQSNLVAPRTKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLDVS
                     VLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKG
                     FSLSL"
     gene            2508..2765
                     /locus_tag="ACHK8E_003517"
     CDS             2508..2765
                     /locus_tag="ACHK8E_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0345733.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENLGKGHSISQVRSEIRARK"
     gene            2819..3091
                     /locus_tag="ACHK8E_003518"
     CDS             2819..3091
                     /locus_tag="ACHK8E_003518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345734.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGVCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            3181..3486
                     /locus_tag="ACHK8E_003519"
     CDS             3181..3486
                     /locus_tag="ACHK8E_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0345735.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NTTISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3529..4269)
                     /locus_tag="ACHK8E_003520"
     CDS             complement(3529..4269)
                     /locus_tag="ACHK8E_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345736.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARKLCSEKPVRNNDFIA
                     RVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDQLESMQQQ
                     LQQKITSKHSTNGLEEFRKARALKMTVDTMKDLFGFLPNLAPEAKQVVAASLVNPVVG
                     ANVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQ
                     VESFRYNNRGVQKIEEILDAENKAEFGT"
     gene            complement(4318..4602)
                     /locus_tag="ACHK8E_003521"
     CDS             complement(4318..4602)
                     /locus_tag="ACHK8E_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0345737.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWINRLVNRKYDRL
                     IITKGYPKRDDSSRRIDTPYDGYKIKTITHTHFGDKPVKVFAIKVNIGSD"
     gene            complement(4595..5128)
                     /locus_tag="ACHK8E_003522"
     CDS             complement(4595..5128)
                     /locus_tag="ACHK8E_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0345738.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALTSLE
                     AEPVAWKVTFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAKPVPVT
                     PDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQ
                     ATHWMPLPEPPQEVNRG"
     gene            complement(5125..5490)
                     /locus_tag="ACHK8E_003523"
     CDS             complement(5125..5490)
                     /locus_tag="ACHK8E_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0345739.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATV"
     gene            complement(5492..6352)
                     /locus_tag="ACHK8E_003524"
     CDS             complement(5492..6352)
                     /locus_tag="ACHK8E_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0345740.1"
                     /translation="MSEIDYQALREKAEKATCGEWSLEYGEGRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEEIALTVGRLLIENGQLVADTLRHLADNEIDSDYFAITSTNENGTEIEHEMAITDYA
                     LQAAGTVDELVAALEAAEKRNAKLQRENAYIRNRYKELDLLIGKNILVMQAAIIEWQA
                     TGDAKSGLAWIYNTLFGPGELPDKSEKDAQAYFNRKYAPIDEKLMALHKWFWEQSEAE
                     RSAGIRIKGE"
     gene            complement(6349..6834)
                     /locus_tag="ACHK8E_003525"
     CDS             complement(6349..6834)
                     /locus_tag="ACHK8E_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFP0345741.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFPVVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLWPYGVLIFKWNETQIPVRQILALTDRKPVIGQRTGKGDKTHWIIFMKEGN
                     Q"
     gene            complement(6831..7532)
                     /locus_tag="ACHK8E_003526"
     CDS             complement(6831..7532)
                     /locus_tag="ACHK8E_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0345742.1"
                     /translation="MTTEIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGI
                     DIDAIHAFKIMAGPETVLSLLDERDALNERIAELEANLAKLAEDQQKAIESIKQADSA
                     VKLAHEKFSELAAENAALKQSEKEFNNFCRQEYYGWEDNFTETPATDAFLAEIRAEAR
                     NEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEPYPIDGLSGEYAEK
                     SLEEWAERLRKGGNQ"
     gene            complement(7635..8078)
                     /locus_tag="ACHK8E_003527"
     CDS             complement(7635..8078)
                     /locus_tag="ACHK8E_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345743.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRD
                     VFVGLYAYDNEKHEEHIQNGCINKFQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(8155..8976)
                     /locus_tag="ACHK8E_003528"
     CDS             complement(8155..8976)
                     /locus_tag="ACHK8E_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP0345744.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADECSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(9040..9387)
                     /locus_tag="ACHK8E_003529"
     CDS             complement(9040..9387)
                     /locus_tag="ACHK8E_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345745.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(9462..9851)
                     /locus_tag="ACHK8E_003530"
     CDS             complement(9462..9851)
                     /locus_tag="ACHK8E_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345746.1"
                     /translation="MDEVIFTYNEESALTAGQGSFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSTLK
                     DKDNRSKNVNPNHTDAPGYWQYGSDSF"
     gene            complement(9851..10540)
                     /locus_tag="ACHK8E_003531"
     CDS             complement(9851..10540)
                     /locus_tag="ACHK8E_003531"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0345747.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(10537..11487)
                     /locus_tag="ACHK8E_003532"
     CDS             complement(10537..11487)
                     /locus_tag="ACHK8E_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFP0345748.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQG
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(11504..11785)
                     /gene="gamL"
                     /locus_tag="ACHK8E_003533"
     CDS             complement(11504..11785)
                     /gene="gamL"
                     /locus_tag="ACHK8E_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFP0345749.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(11806..12087)
                     /locus_tag="ACHK8E_003534"
     CDS             complement(11806..12087)
                     /locus_tag="ACHK8E_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0345750.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVILSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(12099..12311)
                     /locus_tag="ACHK8E_003535"
     CDS             complement(12099..12311)
                     /locus_tag="ACHK8E_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP0345751.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(12382..13155)
                     /locus_tag="ACHK8E_003536"
     CDS             complement(12382..13155)
                     /locus_tag="ACHK8E_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0345752.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNARNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRFGFNVNL"
     gene            complement(13784..14737)
                     /locus_tag="ACHK8E_003537"
     CDS             complement(13784..14737)
                     /locus_tag="ACHK8E_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFP0345753.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGERFLGPYSTE"
     gene            complement(14734..16203)
                     /locus_tag="ACHK8E_003538"
     CDS             complement(14734..16203)
                     /locus_tag="ACHK8E_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFP0345754.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGLQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTHEKSAIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(16298..17011)
                     /locus_tag="ACHK8E_003539"
     CDS             complement(16298..17011)
                     /locus_tag="ACHK8E_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP0345755.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            17107..17310
                     /locus_tag="ACHK8E_003540"
     CDS             17107..17310
                     /locus_tag="ACHK8E_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0345756.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGRVEAEEVRPFPAFVRGDD"
     gene            17365..17634
                     /locus_tag="ACHK8E_003541"
     CDS             17365..17634
                     /locus_tag="ACHK8E_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345757.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            17842..18219
                     /locus_tag="ACHK8E_003542"
     CDS             17842..18219
                     /locus_tag="ACHK8E_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345758.1"
                     /translation="MSTATKSTLIRTLSTVPLRTEERYSFLADVVTLLESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRSRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            18213..19733
                     /locus_tag="ACHK8E_003543"
     CDS             18213..19733
                     /locus_tag="ACHK8E_003543"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0345759.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVRNWLENGEDLPTICFCVNVAHANFVTREFLQAGIGAEVMTADTLHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTAPGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECSKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVHNFIRSKIIAYAKGRKKAMTGGQHAD"
     gene            19723..20694
                     /locus_tag="ACHK8E_003544"
     CDS             19723..20694
                     /locus_tag="ACHK8E_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP0345760.1"
                     /translation="MQTKKAAVGQWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMKLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVMDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDRGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDEHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            20694..21143
                     /locus_tag="ACHK8E_003545"
     CDS             20694..21143
                     /locus_tag="ACHK8E_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFP0345761.1"
                     /translation="MEIEMLKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGCSNNPELNNRLLSFF"
     gene            21151..21714
                     /locus_tag="ACHK8E_003546"
     CDS             21151..21714
                     /locus_tag="ACHK8E_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0345762.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            21711..21905
                     /locus_tag="ACHK8E_003547"
     CDS             21711..21905
                     /locus_tag="ACHK8E_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP0345763.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            21898..22332
                     /locus_tag="ACHK8E_003548"
     CDS             21898..22332
                     /locus_tag="ACHK8E_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0345764.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            22839..23786
                     /gene="stxA1a"
                     /locus_tag="ACHK8E_003549"
     CDS             22839..23786
                     /gene="stxA1a"
                     /locus_tag="ACHK8E_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP0345765.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            23796..24065
                     /gene="stxB1a"
                     /locus_tag="ACHK8E_003550"
     CDS             23796..24065
                     /gene="stxB1a"
                     /locus_tag="ACHK8E_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP0345766.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            24569..26536
                     /locus_tag="ACHK8E_003551"
     CDS             24569..26536
                     /locus_tag="ACHK8E_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0345767.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAI
                     LVHAGRTAEFITGKQPDMVRPTVPSGEGPEREAEAPASKRTLMSLLASGEDLASQGWR
                     YYHKPAGGDSVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGAELLKKG
                     GRVSVRFKLPGSLVPNQFALGIYWQLSSLPEGVMLSGEGNDMLMSFFLQTDTTNLNAM
                     HHRKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGAAFTLVKSPASGAAD
                     KLQLTDISKSATYTLLIDSIAVEVNSTDTAA"
     gene            26671..>26780
                     /locus_tag="ACHK8E_003552"
     CDS             26671..>26780
                     /locus_tag="ACHK8E_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0345768.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQI"
BASE COUNT         6594 a         6217 c         6752 g         7217 t
ORIGIN      
        1 gcgatatttg ttttgtttcc aacgccccct gcaattgctt gccagatgcc gttttttacc
       61 gttacggccc cctggttttt taacttccac agcgtatcaa tgacgtcatt caaatcaata
      121 ttcagacctt tagaaatact ttcagctgat gcttttccta atttgttcaa ttctgtaagt
      181 actatatcca tttttttctc ctgatatttt gatgtcggtg gtgctgttat tcggttggag
      241 aggtgtcacc ttcttctacc agcttttcca gttcatccag ctttcgggaa agaatttcac
      301 caaacagatg aagttctgca tctgctgtaa tcgggattgg aacaaatcgt attccacttc
      361 tggcaagttg atttgcgatt tctagacact gccttaattc aactggtgat gcctttgtca
      421 acgtcgtttt ttcgctcatt ggttttctcc agaaatttaa caatacccgg aaccatctca
      481 atggatggtg tgccgcattg gttaccccat acatcaaatc catgcgaggt gtggcgggcg
      541 aacagttcta tccggggaac atcgccaagc agttgcacaa gcttttctcg agccatatcc
      601 ggcttgcggg agtgatcaag gcgtggtgca gtaaatgact ggattattcc ggcgttaatg
      661 cgttcgggca gatttccttt cactgcaaaa aggcagtctt cgctgttagc gcgagtggtg
      721 ctacccatac ccatgaccag cttgtcggtc tgtcttttcc cgcatttgtt ccaggttaat
      781 cctttcatcg tcaccagacg aaagccccac gcttctacaa ccttcaatgc ttcaagtggt
      841 tgtgtaggca cccaccacat agccaacagg caattatcgg ctgccagttc ccataccggg
      901 aggcggcaga tatccagaag actcatgacc ggatatttaa aacttgcacc gcgttgacca
      961 tcggttgctt tgtcgcggaa tgtccagggg ggatctgcgt aaatgagggt gtattttttc
     1021 gtcatagcgc gttttcaaac tcatcaatgt aaagtcctgc tttaatcagt cggcgacggc
     1081 gttcggcctt ttgcacgtat tcctcgcgac agttttttgc tgcgtgttca cggcttctgc
     1141 gactgaatgg aggaacggca ttgcgtgagc gaggtgtgtt acgacgagga tttgattcga
     1201 gggagaaaaa gcgatcaacg attccggtgt ctgtggtgaa tggttctgaa gcctttattt
     1261 tcacaacctt actgcccttt gtcaggccgc gagctacctt gttgaactcg caaagtgaaa
     1321 caccaaattt ctctgctatt tcactgccag taacaggacg accacgagac tgaatcatct
     1381 aggtgacgcg ttcttttaac ccacggaagg caccgggcct tccagaacgc ctgtagaaag
     1441 cgatctgttt caaatttttg tctccagata tgaaaacccc gacgggatgt cggggatggt
     1501 aggggaagtt acaggcttaa cgagaagcct tttcgggaat ttcgatctga cagtcttgaa
     1561 atatcctgta gcgtgcggcg catttcttcc gtcagatgtt tgaaggcgtt aaattcggcg
     1621 acagcggcgt caacgttata gcccttgctg tgtagctcgt tgaggatgcg catagttggg
     1681 cttggcatat cgaagagcac agaaacgtcc aggttaagag tttttcgatc aacatagctc
     1741 atcatggagt atgggtgatg ctctgaaaac cacgatagag ggtagttgat agatagtgtt
     1801 gattctggaa gctgaagttg ttctgggatt ttttgactga aatagcagtc ttccagtttt
     1861 tcgaacactt cccatgcctg atcggtttcg agcatttttg catgacgggc agcgccgcgt
     1921 tctgtccaga ggatgaggtg ctttgtacgc ggtgcaacta agttactttg agtaaccttg
     1981 ttcttaaatt cccgcaatcc agccccttcc aatttgaagt agtgtttccc acaaacaaaa
     2041 cgctcggcat tgcgtgtata gttcactttg atgttattag tttcggtgcc ataaagttgt
     2101 gccaaaagct cggtggtaat aacaggaatc tggttatggg taatcgggga gatggtttca
     2161 acagagattt gagttgtcat aatgacgccc tctagtggtt tctaaactat caccaccgta
     2221 aggttccaat catcgggtgg tgagacgcac agggttggaa ctaccgggaa accgaccggc
     2281 gagcttttca gctcccccat gcgccccacc ataattcaga tgtgcgcgtg catacgacaa
     2341 taaaaaacac gctcgcggcg tgtgtttgtc gcggtctcta tccggggttc caatcccgac
     2401 gccagatttt gctggcgtgc gaagaatata atcccggata tgtgttgtcg tcaacaagtg
     2461 gcgtgctatc atcgaatagt gttctatcct actcagtgag gtttaacatg cgtacaaccc
     2521 aacaattcag cattacatta accaacgaaa tggctgacat ggtgcgcgcc cgtgtggctt
     2581 ccggtgcata cgcttcagaa agcgaagtta ttcgtgaagg gcttcgtgca ctgaatgaac
     2641 gcgacaaagc aatggaagcg tggttaatac actcagccgc gccgccactc gacgctattc
     2701 gtgagaacct aggcaaaggg cactccattt cacaggttcg ctctgagatt cgcgcccgga
     2761 agtaatctgc atggcatatg aagtgccaca ctaacaaact gctctaggta tgggaatagt
     2821 gtcacttgca gcacctatta gagatccgat aatgaacatc atagcaaggt tcttttattt
     2881 gataattttc tgcatggcaa catcaggatg cactaccact acgaaaataa atcgcggaga
     2941 tgaaaaggaa cagtacatca ttgcctgtgg tgccgctaca ccatggggag tatgctatga
     3001 taaagccaat gccctatgta aaaatggata taaagacatt ttaaaggaac agggatttaa
     3061 caggaaagaa ttaaccattg agtgtaaata aggccatcat ttgcaacgtt gaaaaaattc
     3121 gcccgtgcta cagggaaaaa actccagagc cgcttcgttt aactacgagg attaccacta
     3181 atggcgatgt ttaacccccc tcatcccgga gaaattattg ctgatattct ggaagatcag
     3241 aatattggaa tcagggaact ggcaagagcg cttgatgtcg cgccttccac tgttcagcga
     3301 ctggtttcag gtaatacgac aatatctcct gaaatggctg ttcgtctcgc tgctgtcctg
     3361 ggtggaactc cgtcttcatg gattcgtctc cagacggcat ggagccttga aaaagcggaa
     3421 agagaagttg acgtatctca tctctcaaca aaataccgcc cggcagaaat ttcgcctcat
     3481 gcttaaccac cgcgccgtca ttctggcggc gtcggagagt ggaggatatc aggttccgaa
     3541 ctctgctttg ttctccgcgt ccaggatttc ttcaatcttc tgtactcccc gattattgta
     3601 tcggaagctt tctacctgct tatccgaata cggggatttg tcgatgaacc atttcccgta
     3661 ttgttcggtt ttgagcatgt aggtattggc aatgcgacca accttgttag cggatatttt
     3721 gagttttgct ccaacttctg acgccgaata gtaatgttca ttgatcatcg gtagcgggat
     3781 tacgttagcg cctacaacgg gattaaccag gctggcggca acgacttgtt tagcttcagg
     3841 ggcaaggttg gggaggaagc cgaaaaggtc tttcattgta tcgacggtca ttttcagcgc
     3901 ccgtgcttta cggaattcct caaggccgtt agtcgaatgt ttcgaggtga ttttttgttg
     3961 cagttgctgt tgcattgact ccagttgatc gaccagcgaa cgacggacgg ccttcgactc
     4021 acgcgcggct actcgcaggg cttgcttgaa agtcattgtt ataacttcta tttctgcgcc
     4081 gtttttctga cctacgaaaa tttcgtaggt ctctccttca agctcatcct taactctggc
     4141 aatgaaatca ttattgcgaa cgggcttttc actacataat ttccgtgcct cattgaccat
     4201 tttcaacagc atctggctgt cgattgttgc agccggaaac gaagactgat ttgcaatatt
     4261 gttaatcatg tattctgtcc ttttcagata aaagaaatcc ccgcgagtgc gaggatgtca
     4321 atcactgccg atattcacct ttattgcgaa cacctttacc ggtttatctc cgaagtgtgt
     4381 atgtgtgatt gtcttgattt tatatccgtc atacggggtg tctattctgc ggctggaatc
     4441 gtcgcgcttc ggatatccct ttgtgataat caggcggtca tacttacggt taacgaggcg
     4501 atttatccag tagtcattac acaggcgata ctcttccgtt ttctctccgc gaatcatggc
     4561 atcgaagtat tcacctttga cggcaagttg caggttagcc acggttaacc tcctgcggcg
     4621 gttctggtag cggcatccag tgagttgctt gctcaatacc attacccggc ttaatcgttg
     4681 catctccgcg ccgaaaggtg cttccggtat agcgtgcgga gcatattagc ggttcaacca
     4741 gagagctatc gaaattcacc gaaataagca cgttctggcc cttttcaggc attcgctcac
     4801 tacagcttat ccaaccatcc ggagttaccg gcactggctt ggcggtataa agcggtgtta
     4861 tatctgcccg aaaattacat gctttatgca gccgcaccca ccgttcgact tctgctttgt
     4921 cagaatacat accagtgaac gtgttatatt cacggtcaat ttgcgtgaat gttaccttcc
     4981 acgccaccgg ctctgcttcc agcgatgtca gtgcaatccg cgccagttct tccgcttctt
     5041 ctgctggcag tacaacgttg ctacccggtc cgtatgtttc gcgccactgc ttgattgtaa
     5101 gtagtcgctc tttggtaata gtggtcatac cgtagcccct tcttgatatt tttcaaacca
     5161 gaacacaacc gggtcagatt tcatttcaac caatcccata cgaaccagcg ccttgccttt
     5221 cccggacgca aggaattcac gacgaccatc actgataatt cgccgataat cttccaggct
     5281 actgcaatgc ttgtgcagat tgcatgggtg gcatgccgga accatgttgg atatatcgtc
     5341 acgttcctgg tgaagcatat ttccatcaaa acgaatgacc ggttttacat ggtctgcatg
     5401 ccacttttcg ccaagttcac agccgcaata agcacagcga ccgccaaact tcatgcgcaa
     5461 ttctgcacgt tgttttttcg tcagtgccat ttcactctcc tttgatgcga atgccagcgg
     5521 agcgctcggc ttcactttgt tcccaaaacc acttgtgaag cgccataagc ttttcgtcaa
     5581 tcggtgcata tttgcgatta aagtaggcct gagcatcttt ctcagatttg tccggtaatt
     5641 cgccagggcc aaacagtgtg ttataaatcc atgccagtcc gctcttagcg tcgccagttg
     5701 cctgccattc gataatggca gcctgcatga ccagaatgtt tttcccgatt aacaggtcca
     5761 gttctttgta ccggttgcgg atgtatgcat tctctctttg taattttgcg ttgcgcttct
     5821 ctgcggcttc cagcgccgca accaattcgt ctacagttcc ggcagcttgc agtgcgtaat
     5881 cggtaatagc catctcatgc tcaatttcag taccgttctc attcgttgag gtgatagcaa
     5941 aataatcaga gtcgatttcg ttatcagcta agtggcgtag cgtatcggca acaagctggc
     6001 cgttttcgat tagcagcctc cctaccgtta gcgcaatttc ctcgttctcc tggtcgcggc
     6061 gtttgatgta ttgctggttt ctttcccgtt catccagcag cgccaagacg gtagccggat
     6121 tggctgcggc gatgaattca gcattggcct gctgttccat ttggaaatct tcatcgaaac
     6181 cgctttcagg atgcgctcct tcaattctgc aaatgggaat atatccagca gcctcgcgat
     6241 gaattagtgc atcatcacca tcaaatcggc cctctccata ttcgagcgac cactcgccac
     6301 acgttgcttt ttctgccttt tcacgcagtg cctgatagtc aatctcgctc attggttgcc
     6361 ctccttcata aaaataatcc agtgggtctt gtcacccttt cctgttcgtt gaccgataac
     6421 aggctttctg tcggtcagtg ccaatatttg gcgaacaggt atttgcgttt cattccattt
     6481 aaaaatcaga acgccgtatg gccacaacac acgaaaggct tctttaaatc cctgccgcaa
     6541 atcatcacgc caggtatctt tattcagccg tccatatttc tttcccatcc aggcgttatc
     6601 accaacacgc tcaagatgcg gagggtcgaa tacaacaacc ggaaacgatg cgtctgcaaa
     6661 tggtaatgca cgaaaatctg ctatcaggtc agggctaatt atcagccgtc gtccatcaca
     6721 caatgtgtgc tcttcctttc tgatatcgct aaatatcgcc cggtcgtcat tcttatcgaa
     6781 ccagaacatg cgactgccac agcacatgtc gaggattgca gcatgtccag tcactggttg
     6841 cctcctttgc gaagccgttc cgcccattct tcaagggatt tctccgcata ttcaccggac
     6901 aggccatcaa tcgggtacgg ttcattagcc aactcttctt tcgctgacag aatcatgcgc
     6961 gtaacgtcga aaacttcacg caaagactta ttgataaatc cgtgattgaa cgcagcagca
     7021 agacggctgg cggtatagtt aatcccctcg ttgcgtgctt ccgcacgaat ttcagccaga
     7081 aaagcatcgg tggctggggt ttccgtgaag ttgtcctccc aaccgtagta ctcctgacga
     7141 cagaagttat taaattcctt ctccgactgt ttaagcgccg cattctccgc tgccagctcc
     7201 gaaaacttct cgtgtgccaa cttaacagcc gaatcagcct gcttaattga ctcaatcgct
     7261 ttctgttggt cttcggccag cttcgctaaa ttagcctcca gttcggctat gcgttcgttt
     7321 aatgcatctc gttcatccag tagtgacagc acggtttctg gcccggccat aattttaaag
     7381 gcgtgaatcg catcaatatc aataccaagc tccttgagct tcttttcgct taataaatca
     7441 tcatcacctg gtaatattga cagatgtgtc atcgctgtaa ttgcgcgttc cgacacgtca
     7501 cgcaatacct gatagtcaat ttcggttgtc atattttcgt ctcgttatgg gaggggaatt
     7561 ggttgtaagc tggaattgcc ggatgtttac ccgtgtccgg ggtacgacct cacgttgcag
     7621 cgtacgagaa tggactattg ctcagtgtcg tttgatgtcg gctgaggata ttcgccttgc
     7681 tcttgtaaca gagttagaag aatggaaaac ccctcacgat gtgatgattg atgagtgtcg
     7741 atcccggaat gattgatgtt gttaaattcc aacatcgtgc tcctggcgat agcctcaacg
     7801 gtattccaga atactaatac atctgttttc tgctctgttt tatcagcaac aggaagtttg
     7861 atattttgtt gaaatttatt aatgcaacca ttttgaatat gttcctcgtg cttttcattg
     7921 tcataagcat acagcccgac aaaaacatct cgcacgttac gggatatatt ttccagattc
     7981 gcctttggtt cctttgttaa atcaagcacc gataaatggg ctttaagcgt atccgcagct
     8041 attttctgaa tatcctctgg taaatcttta aattgcatag tataccctcc aagaactgtg
     8101 tattttctcg tgaaatatga gcgatatgtc gtgacgtccc tgtcacagat ttcatcacac
     8161 tgagaacgtg ccgatgaagg ttaagatatc ttcttcttcg aaacgctctt tcagcaggtc
     8221 acggaactct ttggcaattt cttcttccgc agcttcaaca cgcacgatac gtagcaccag
     8281 aacaggctgt tcgcttgtca gaatgcttaa tcgcagcgtg aattccctgt tgcccaggcc
     8341 ctcgtatggg atgcattcaa agcggaaagt ggcaggcatg atttctttgc ttttagcctc
     8401 cacggactcc atgactgagc ggctaccgct gaagttttgc tcttcatatt cagcactgcg
     8461 ttttgcatcg atggtaagac gacggacagc agagatagcc tgtttgatat ccagcacatt
     8521 accgtcgctg tcataggctg tgaggaagtc ggcccagtct tccagccatt cagcgagaga
     8581 cttctggagt tgtcttttgc cgtccacgtc acgtaatgcg cgatatggtg atgtggcttt
     8641 cagttccagc agtgccttgt tgtcagcgtg tccaggttgc tccagagtgc cgatattgaa
     8701 cacagtgact gctttcatcg tttccgcgtt aatgaagcag cgggagcact catcggcata
     8761 ccctttgctg tattcaacaa agtcatcaat actggttgtg gtcatgactc cacggaaacg
     8821 aaaacgttct gtcatgaatc gttccagact ttcgatattt acattgtccg gcaataacgc
     8881 aacagggcat tccgtaagcg gcaaattttc accagaaaaa gcagtagtgg tgagttttac
     8941 aatttcttta atggcattgc tatctaactg agacatgtat atttaccttt aatttatgta
     9001 tttagggata atgcgcgagc gttttaaggc ggggaatatt cagcgtaaaa cagcgtcatg
     9061 ttggccctgt ttatcaaaaa gctgtccctg gtctttctgg aacaaagtaa gctcaccacc
     9121 tttatttaca aacatcgggg tgttagttgt gtcttcttct gatttactac cacgcattgt
     9181 cgggcgtgtg aattttagtt tatgggaaac tgatacgcga ttttcatcaa gtgaagataa
     9241 ttcgaattca acacatacct ttcctttttt gtttgtgctg tttacgccaa aggcaacttc
     9301 acttaatacc gcgccaagtt tgttaacaaa taccccgccg tcgaggtcgt taatgaaaac
     9361 attaacatca gtttgatgct ggttcatatt aatatctccg tgtagaggtg tgttaaggct
     9421 tcagttgttc actgcattta aagcctggtt ttgtttgtga attaaaaact atcgctgccg
     9481 tactgccagt aacctggggc gtccgtgtga ttcgggttta cgtttttgct gcgattgtct
     9541 ttgtctttga gagtggaaac catatgttgc gttaactgcc caattcctgt gcatcccatc
     9601 atggcgtgaa taatgctcgc cccaaattta ttttcagtac cgtcattctt ctgaacacaa
     9661 acactgagat attggatttt ccgtccgtcg tcggattctc cagaaaactc aatgaatcgg
     9721 gcaccttttt ctgattgctt gagttctgct tcggtgatgt taatgatatg cgcacccgtt
     9781 tcggtaataa aactaccttg tccggcggtc agtgctgatt cttcgttata agtaaaaatc
     9841 acttcatcca ttagttatta accccccatt gttctccaaa tacaaaacca atttccgcca
     9901 gtgcttcgtc cattttgtcg atgaactccg gcaccatctc atcaaaactc gccatgtact
     9961 tttcatcccg ctcgaccacg acataatgca ggccttcacg cttcatgcgt gggtcgtagt
    10021 tggcgaagta ccaggcgtcc ttgtctgtaa cccacatgct gaattgcacc tgggccatgt
    10081 aagcaggctt gatagcgtca aagccgccaa gccggaattt catgaagtcg cgggaagtaa
    10141 aagggcattt aagctcaaga ccgttaccgt cgctgcataa accgtcggga gagcaggcgg
    10201 tgcgcatgct ttcgtcacga aaaattattg gcgtttccga gacggtaaca tccgcgataa
    10261 attcgaagag ggcacgggca tcatcttcgt attgctttcc ccatgcgagc gacttagcgt
    10321 taacttccgg tgccacgcca gtacaaactt cagccagcag ggtgtggaag tatgacattt
    10381 ttgtatcagg ccatttgctg ccggaacgtg gctttgctat cacattgtga acttctgaag
    10441 cagtgataac accgagtctc agcttgtgcc atccatcatc gccctggtcg aggctggtaa
    10501 tatccacgcc agttcgctgg agaataattt ctggtgtcat gacacggcct cgctgttatt
    10561 ttccgtggtg gcgtgaattt tcgcttccgc cgtggccttg tttctggcag ctttcttttt
    10621 gacaaaatca agcgttttga cagcttcttc ctgactgaga tattcatgtg atgcaatcgt
    10681 gcgacggaat gttttggaac ataagggaag caggtcatcc catgtcttgt taatttcggt
    10741 gattgcctga gtgatctcat tgataatctc atctgatgcg ggagtgacgt cacgctcagg
    10801 gatgtgatca gcattcagga tgataccttc accgccctga gtgttcaggt agtcgatagc
    10861 tgtatccagg cgatcgcgac ggggccagta tttgctggcg cgcttaacaa tggcttttct
    10921 ggccatttca tcggggaaac tatcccacgg gctacttcct ccgttatttc cggctttgct
    10981 gcacgccctg atgacctcga tttccctgtt gctcatctct tcggtcagat agtcgccttc
    11041 ggatgttttt acgacacaat aaccgccaat gcgtgcgccc ctgtctacga atgggttgta
    11101 tttgtgcgtg ggggcgcagt caataccgtt ggactcgtaa atgtccttct cgtaaacaag
    11161 tttgcattgc ccccactgaa tggctcctgt gacctgtgcc agatgcagaa gccccatata
    11221 actgatatcc aggcatacag ccttttttcg tgggaccaga tacgccagct tgctggccgg
    11281 gttcagggtt atgccgatgg cggcaacatt gatgatagcg ttctgtgcgc tgggcagatt
    11341 tgcccgtgct gtgtcagcca gaaaggcatt tttctggaat tgctgaattg caaactgact
    11401 ttccttcgcc catgtcagcg tcggttcagt taatgcctcg cagaaaaagc gctcctgctg
    11461 cttaacaaat tcaacgatat cgaacatttt ttggtcctga aaatcagaaa ggacaggggg
    11521 agaattttct ctcccattct tcttccgccc gagcataggc gatcgctgag atataatcgt
    11581 tgtacgcctc ttcagctttt tcgccagtga gtgccagttg ggcttctttg ggtaaaaaaa
    11641 ggctgctcat aagcaatggt ttatcgggga acatgctgat aagctcctgc gcccgatcat
    11701 caatccattt atccttttca tcctgaattt gctgattaat ccagcgacgc tcctctatgc
    11761 ggtcgcaggt gaggtatgcg ttcatggcgg aactcctgat tccggttaat gcattaaatt
    11821 aatttgtcgg gaaagctgac atacagggca gttacattct tcctcctgct ccttagcgaa
    11881 gaaatatgca gcggcctgta atgcgatgtc ttctggatgt tccgcgataa acataacatt
    11941 gccttccgta tcaataacag aaatagcctc atcagacagg atgacaaaat aggcgatgat
    12001 tttatcatcc ataaaaactt ctcccattat cgttcctgct ggagttacga cgctttttac
    12061 attgatattt attttttgat tgagcatgat atttcctttc aggctggtga gattaacggt
    12121 tggcctttat tgttcaggta aacttctatt gcatctgaga taatgcgaat tttttcaatc
    12181 agtgaatgtg cgtaaagtgc attattaacg ttcgctgacg ccatgtaata acgcccgttg
    12241 taaagaattg ctgtgccggg tttaacgtcc tcgcgagaaa ctaatgcggt tccgtagtga
    12301 ggtttgagca tgacaaatcc tccggttaat taatccagat attttattta atccccgata
    12361 tgtgatcggg gatggggtta attaaagatt aacgttgaaa ccaaagcggg aagatttttc
    12421 cgatgtgcgg gaaatatcca gcaatttacg gcgcatttct tccgtcagat gcttgaaggc
    12481 gttaaattcg gcgacagcgg cgtcaacgtt atagcctttg ctgtgtagct cattgaggat
    12541 gcgcatagtt gggcttggca tatcgaagag cacggaagca tcaaggctga taaccttgcg
    12601 atcaacatag ttcatcgtgg cgtaggggtg atgctctgaa aaccacgaca aagggaaatt
    12661 gatattcatc gcaggagaag acagggccag ttgtttctgt tcccatagtt gtttttccat
    12721 gcgatcgaat tcagcaatgt aggcttcctt gaatgcagct gcttttttgc cggtgaagcc
    12781 catcaccaga aaaacgaagc cgtttttggt gatttggtac attgggcgtt gttcgccttt
    12841 ggcgtcggtg taggtaacgt ccgcaaaatt gcgggcgtta aattccgtag aacaatccat
    12901 caatcggatt ttttttaaaa cgtcgtcgtg ccgcttgtca aaaaattcag caatagcaat
    12961 agacgttgtg acaacgcgac cattggtaat ggtgatttca ggttgagaaa gggcagggat
    13021 agtagccata atggcagcct ccttgattgg tgattgagaa ccaccgcagg aggtcctaag
    13081 ctcgctggcg gtggactgta cagggttagg actactggca atcaagggaa ccagcccgac
    13141 cgaagtcggc cccatacagc ccaccattga taagatgtgc gtgtatgtcg atacaaaaaa
    13201 agacgctggc gcgtctgtat cgcctcgatt gtcagcgggg tcctaatccc gacacccgtt
    13261 ttataaggtg ttcgccaaat atagccccga catcacacgc agtcaatacc gtcctttctc
    13321 agaaacgctt tggcggtgcc gcctgcaccc caaaacattc cctgtatttg gtcagcgcca
    13381 acttcctgcc agtgttgccc gttctcacgc cgttctcgct ctcgcgcggg gatactctct
    13441 catcgaccgg atcgcacccg atgatacagc acgtttacgt gtaggggtct aaacaggtca
    13501 ttgacgctgt aaagctccag attgttaaag agcattttgc ggcgggttaa gtcgcgccgt
    13561 acgactgatt tatgtagccc tgtgtaaggg cgcgatgttt ctggcttgaa acaaatataa
    13621 cttgcggtga ttttcatgta aataccgctg gtgctttttg gggcgaaaaa ttattactta
    13681 actgattttt aaagtgattt attttttaag gggacagata acaggagggg atatgcaaag
    13741 aaaaacccgg cgcatgggcc gggctattta tctggtcctg tactcattca gtagagtagg
    13801 ggccaagaaa tcgctctccg ttgaagtgaa taagatgaga aggtgcatca gccacccata
    13861 cctctgtttc ccatgcgatt tcaccaagat atcgtcccat gatggagcga tttggaaaag
    13921 cggtcacata gacaagtccg gctgttgatc cggcaaacag cctggcaagc tcagcatgcc
    13981 gcttcccatc aaccggtcca tgactggtga cagactcaac cagtagcagc cagtttttcg
    14041 cagtaaaatg tagcaccaca tctggcattt taccgtgtga atccacatca acacctagcc
    14101 cggccagcag tggagcgtcg aagtagcccc acttttcgcc agtgtcgcca gcatagacca
    14161 gcacgctacc cggagcaaaa cgtggggcga agtcctctat tatggcacgg ataagttcgc
    14221 tgtgctcgcc gggactgagg gttatttgct ggcccgcagc aatttcaaca gggatacgat
    14281 tctgttcgcg ctccttagca tagcgggtaa ccagcgtttc acgctcggct aaataggttg
    14341 caaggctatc gtgccatgcc gggctgccga aagtgcgtag catggacaga gcagcaggtt
    14401 cgatctgata aacagccttc gggctgttca ctgggcgatc gggcttgtcc ggattgtaga
    14461 gggctactcc agcagcgcag aactgatgca tagactggcg gcggaatgtc tcacgagtat
    14521 tgggtgcgta gaccttgccg tagtgctccc gaacccaatt catgattggt gtaatgccca
    14581 caagcggatt ttccgcgtcg gcccatgctt ttcccggtgt gaggttcagg agtgccagca
    14641 aacacaaagc agaacgctca ttctgctgcg ctctgggcag gcctaaggat gctataattt
    14701 gctgagcggc ctcaatataa tcattttggt tgttcatgca gtcagagctc ctagcttagc
    14761 gtcaatatgt tcttgcgtga gagtttcttg ttgcatagcc cacttgccaa gctctatcag
    14821 agtgtcacgg cttgggtact taatcatctt gaggtcggtt gcattgacct gtgtatgccc
    14881 gttgaagcga cggaaatact tatccactgc ggtagtgttc aggaacacgg ccaagccacg
    14941 ggccagcgcc tcgggtaacc catgtttgtt ctcatgaaac acgttcatgt gattttcaaa
    15001 tcccaacact gaatactcac tgaaggtagc aggatcaacg acgcttgcta ccactcggcg
    15061 cttttcctcc ttcgacgaga aacgacgcac cacacaataa aaaccgttcg ggtaaagcca
    15121 cttttctgtc tcgtcgttgc gcatgattgc gttgggtttc ttcaaacctt ccacaggcca
    15181 gacagtgcta ctcatactca aatggctcgg gtagatcagg ggaacagttc cttcttctgg
    15241 catgctgcgc aaatgagctt tcagtcggaa atcaactatc ggtccggtcg ataccttcac
    15301 gccaatatca gccagcgaac attgtacggc ggatgataac tcaatggcgc ttttctcgtg
    15361 cgtggtcggc acatgaataa accgctccga atcgtccggg tacacaattt ggtcaaatgg
    15421 gtgttcgtag gtgaccaggt caaaaaagct atcatcggtc gaagttgtga ctgtaacagc
    15481 ctcctgaagg cctccacgct ccaggcggat gataatattt tcctgcaaca catcgtcatc
    15541 cctgaaagct ttgctacgtg attcgaacaa atgaatatgg cggattgctg cccgctcaag
    15601 tatgaaatta cggaacggac ggtaatacgg tccgttgcag aaactacgtg ggattatggc
    15661 cactatctgc ccgccaggtg caacctcacc tacagccagt gcaacaaagg cggaatacag
    15721 atttaccgtc tcgataccga cagtgcgaag agcctgccgg tgggctgact ggctattgat
    15781 cttcttgtaa ggcggattaa gtattgcatg ggtataacct cgatcctgta gaccttcggc
    15841 ggttgccagc tcaatataat ccccttcgat gatgcggggc gtcactctcc tgtacccggt
    15901 taagtgcttc gcaaggtgac cacacagctt tttgtcgatt tcgtaggctg ttgcctcgac
    15961 agactcaaag ccaaactcgc caaccagcca acggtcgagg aaggcgcaag acagtgcgcc
    16021 tacaccagcg cctgcgtcca gtagacggca agttttcatt gtgctcggtg gaaacaagga
    16081 ggccataaaa cgggccacgc cagaaggcgt catgaactgg ccgaactcag ctttgtgttt
    16141 acgcgcggta cggggagaca gctcgcgacg cacgctgtct gcgacgtcta gcttttgaag
    16201 cataactaat cctctttacg tggcatgatg atgccaacgt gcttttgaca tcctccacat
    16261 tctgcaggcg tgaaggatgt caaatcactc tgtgagctca atcaaaagtc tcatcattcc
    16321 actgcgattt gatgaccttt ccgacaaact ggcagttttc gttacactca atcatgtcga
    16381 atcgtgggtt gagcggctcc aggtacgctt taccgctctc acgaatgaat cgtttgaagg
    16441 taaattcatc gccgttcatt cgggccacgc aaaaatcgcc atcctcgatt tcccgttcag
    16501 ggtcaacgag tatcagcatc ccttcgggga aactgggttt accaccctgc ggcgcggtca
    16561 ttgaatgccc cttgacctcc agccagaacg acctgtcact ggcttttgtg gtggtggaga
    16621 tccatttaat cgcatctttt gcggtgtacg aaatatcatc cattgagaat gcgccagctt
    16681 gtacgcagga gagtaacgga tattcgtaag cggggacacg agttaatggc tcttcaattg
    16741 cgagcgccat ttctgcaata gattttgcca ggctgggact gaaatctccg acctttacat
    16801 tcagtatctt agcaagctgt gctgcgtggc tggcatttat ggcgttgatg ccagccatta
    16861 gctgattaac agcgctttgg gtaacaccta attcataggc taatgattcc tgcgacaggc
    16921 ctaactcttt ctttttagcg ttaaaaatac ttttcagacg cttggcgtct tccagttgct
    16981 caggggttaa cggtttcttt ttcatgttca taccatatca cctttggtta tatgtaatga
    17041 aatatctgcg gtgttgacat tttaataact gtgcgtaatg atgtgggcat tttgaaggag
    17101 tcagttatgg cgatgaaaaa agagaccctg gctgattacg tatacaggtt cggtcagaag
    17161 aaagccgcta aggactttgg tgtggcgcag agcgcgatca gtaaagcgct tctcgtcgga
    17221 cgtgaaattt acgtcaaaac ctttgatgac ggcagagttg aggcggaaga ggttcgaccg
    17281 ttccctgcgt tcgtaagagg cgatgattaa tcctggacac acagcccatc ggaggtgtga
    17341 gtgaacaggc aaatcaaaac ggttatgccc gagcagtact gtgatgctga tcgggactgg
    17401 atacaggagc aattgttaca gctagacccg accacccggg taaaaattgc agcagagtac
    17461 gcgagagtgt accaggaaga gtgggacaag gagcctgtat cgttcaggaa gggcaacagg
    17521 gcaagacgga gcgccaacac ccggttacgg gtgtatgtcc agaagtacgc gagagccagc
    17581 cgtggctgga tgctttcgcc agtagcggta cggaagtttc ccccgacacc atgatggcag
    17641 ttagttgcga tagagataat ttcacatgca actaatttcc tgaaaggaag cgaggagggg
    17701 gtaagggggt ggagtcgttg gtaaggcctg tatcccttgg cctaggccag aaggcaggta
    17761 cataggttag gtagatcact gtataagcgc gcaatagttt ctggtgaaat aattatcaag
    17821 ctaattattg agtgataaac tatgtcaaca gcaacaaaat cgacactgat ccgtacactg
    17881 agcactgtac cactgagaac agaggagcgt tacagctttc tggctgatgt tgtaacgctc
    17941 cttgagtcac aggggatgca tgtggcaagc aatgtcacag taagaatcga tggcagaaat
    18001 ttccgtgttg atattctcgc aacagcaaaa actggtggga gtgtggctat cgagatcgat
    18061 cgtagctctc cgcgatcccg ctcagtcatg aaattgcgtg aattggcacg tcgtggcact
    18121 gaaggctttg tgttgcttcg aatgccaaag aaactgacca gttatagcga tgctgggatc
    18181 gatatcatcc cggcgaatgg caagggggcg tcatgctgaa aatcacccca aactttgctc
    18241 aggagcgcgg tttaaaccag ctgcggcacc agtggaaaca gcatcgcacg tacctgatgt
    18301 acgcacccac tggctctggc aaaactggtc ttgcagcatt cgtcaccgcc ggaatggttg
    18361 agcgtggtat gcgagtgatg tttgtctgtc cgtacaccat cctgctgaac cagacagcgg
    18421 aacgtttcac cgaatacggt ttgccgtggg aagaaatcag ttttgtatgg cgtgatcacc
    18481 ctaactatga cccgtcacga ctgattcaga ttgccagtgc cgacacgctg atccgtcgtg
    18541 agtttcctga caacatcgat ctgctgatca tcgatgaagc acacatgaag cgtcgtgcgt
    18601 tacttgaggt tatccgggac agagacatca gggttcttgg actttccggc accccgttcg
    18661 cagcctggat ggggaagtac tacgaatgcc tgatcaagcc tacaacgatt cgtgaactta
    18721 ttcagcgtgg tgatctgagc gattacgagt tttttgcccc ttcaatgcct gatctggctg
    18781 gcgtaaaaac cagtaacacc gtatttggac gggattacaa cgaggagcag ctcgcatcca
    18841 tcatggggag ttcggatctg gttggcgata tcgttcgcaa ctggcttgag aatggcgagg
    18901 atttgccgac gatctgcttc tgcgtgaatg ttgcgcatgc gaattttgtt acccgtgagt
    18961 ttttacaggc cggtattggc gcagaggtga tgacggcaga taccctccat gatgaacgac
    19021 aggacatcat tcgccgtttt gaagagggcg cgaccaaaat aattgtcaac gttggtgtac
    19081 tggttgcggg atttgacagc gatgttcgct gcctgatcta cgcccgtccg acaaaatcag
    19141 aaatccgttg gttgcagtgc ataggtagag cgttacgtac agccccaggt aaaaaacgtg
    19201 cgttgatttt cgaccattct ggcacggttc atcggctggg attccctgag gatatcgagt
    19261 atgacgaact tccgggaaaa aatgacggaa tgaaagcgtc tgctggcggg agtgaggtta
    19321 aggctgagaa acttcccaga gaatgctcca aatgccactt catgaagcct gctggtgtac
    19381 acatgtgtcc caaatgtggg tttcgtcctc ttggtggcga tgatgtggca acagaccgcg
    19441 atcgaaaact ctcacgcgta aataaaggga aacgcgaata cagccgcgag gaaaaacagc
    19501 ggtggtggag tgagatcaag gggtatcaga attaccgcaa cgcgacaggt aagccactga
    19561 gtgacggatg gtgcgctcat acctacaaag agaagttcgg ggtgtggcct aaaggcttca
    19621 gtaacgcgcc gctgcaaacc tcggttgaag tgcacaattt catcaggtca aagatcattg
    19681 cctatgccaa agggcgcaag aaagccatga ccggaggcca gcatgcagac taagaaggcg
    19741 gctgtcgggc aatgggggcg aatttttgaa tattacggca tgcctcctgt tactggtgtg
    19801 aaacattaca acggcccatg cccgatatgt ggtgccagag gtaaatttcg ctgtgatgat
    19861 aaagatggct ccggttcgtg gatttgcgtc tgtggtcacg gggacgggat gaagttgttg
    19921 caacttgcca cgggtaagcc ctgggtgacg ttgtgtgatg aaatcgatcg gctgatcgga
    19981 aatacctgga agagggagaa agtcagccag cctgtaacag agataagcag aaagcgggag
    20041 ctggtcatgg ataagtttgc cggactcccg tgtctgcggg gcacaacagg tgaggcgtac
    20101 ctgcagggga ggggaatact ccagttaccg accgaatccg tgcgtttttg tgaccgtcag
    20161 atcgccagcg ggcgcgaata tcaggcaatt tacgccattg caacagatga cagaggttct
    20221 ctttgctatc tgcatcgtac gttgctggat ggtgatcgca aggcgaatgt agaggcggct
    20281 aaaaaaatga ccgcgctaca ggagttgcct ggtttgcagc atgccaaatc ggtggcaata
    20341 cgcctgtatc cggtgtcgtc cactctgggg atagccgagg gtatcgaaac tgcgctttca
    20401 tgccgtcaaa tcttccgctg caatgtgtgg tcaacaatga actccggttt tatggagaag
    20461 tttattgcgc caccaggcgt taatcacctg attatctttg cagacaatga tgagcacggc
    20521 gcaggtctgg cggctgcctt taaatgtggg cataagaatc tcatgagtcg taatgacgtt
    20581 gagaaggtca gcattcgctg gcctgacttg ccggatttta acgacatgct cattcagggg
    20641 tgtgaagccc gtgaacatgt gttgacgcgc aaattcaaag cggaggctgc ctgatggaaa
    20701 tagagatgct caaggcggct aatggcgtat ttgtaccggc gtatgagcgc gatttacccc
    20761 gactggcaaa atttaaaaac ggtgagctgt atacactgga agcaaaactt acccgtaacc
    20821 catcttttca ccggaagatg ttcgcttttc ttaatttctg ctttcagtac tggtgcgctg
    20881 aacatgctgg atacgaattc tctgatgaag cgacgcagtt tgatgagttt cgtaaaaatc
    20941 tgacaattct tgccgggttc tatgatgtgg tcacaaccat aagaggcgag gtgagatatc
    21001 gggcaaaaag cctgagttac gcgaatatgg atcaggatga gtttgaacgt tgctacaacg
    21061 caatgattaa tgccgcgtta aaacatgtgt tcgggtgctc aaacaatcct gaactgaata
    21121 accgcctgct gtcgtttttc tgaggtgatg atgaagcaac gtaaaccaaa aaaatgcaaa
    21181 gtgtgcggct cctcgtttgt gccgttccgc tcatatcaga aagtttgctg tggtcagtgc
    21241 gcactggaac tggtcagaaa agaaaaggcg atagcttcag caaaagagca ggcagacaag
    21301 ctgaaagcgc gcaggaggga cttacagccc cgcagttact ggattaagca ggcacaacag
    21361 gcggtgaatg cttatatcag ggagcgggac cgtcatttgc cgtgtgtttc atgcgggacg
    21421 ttcgattcag cccagtggga tgcaggccat taccgtacaa cagctgcggc acctcagctc
    21481 agatttgatg aacgcaatat ccataagcaa tgcgtggtgt gtaaccagta caaaagcgga
    21541 aatctcgttc cgtatcgtgt cgaactgatt aaccgcatcg ggcaggaagc agtagacgaa
    21601 atcgaatcaa accatagtcg ccaccgctgg actgtcgaag agtgcaaggc gatcaaggca
    21661 gagtatcaac agaaacttaa agacctgcga aacagcagaa gtgaggccgc atgacgttca
    21721 ccgtaaaaac cattcctgac atgcttgttg aggcatatgg aaatcagacc gaagtggccc
    21781 gaatactgaa ctgcaatcgt gccacagtca gaaaatacat tggcgataaa gaagggaaaa
    21841 gacacgctat cgtcaacggt gttcttatgg ttcaccgcgg atggggtaaa gatactgatg
    21901 cgtgatatcc ggcaggttct tgagcgctgg ggggcatggg cggcaaataa ccatgaggat
    21961 gttacatggt cgcccattgc tgccggattt aagaggctga tccccgaaaa agtaaaatca
    22021 cgtccacagt gttgtgacga tgacgcgatg attatatgcg ggtgtatggc tcgccttaac
    22081 aggaacaaca gcgatctgca tgacttgctg gttgattatt acgtgttggg ggagacgttc
    22141 atggcgctgg cacggaaaca tgggtgctct gacacctgta taggtaaacg ccttcacaaa
    22201 gcggagggga ttgttgaagg catgctgatg atgctgggag tgaggcttga gatggatcgg
    22261 tatgttgagc gtgaattgcc gggagggaga acctctgtat tttatcagcg aaaaaatagt
    22321 ttacgatcgt aaaaatctgc atatcatgat aagagtggtt acattgccac gctgcttaac
    22381 ccgccgatgc gcgggttttt ttgtacccag aatcctgtga gctatacgga aagtacacag
    22441 aaaggaaggt gcgaccacaa ttaataacaa aatcttaaaa atcgcacatg gcactattag
    22501 ttttctaaat attgtgtatt ttttgtattg caggatgacc ctgtaatgaa gtttgcgtaa
    22561 cagaattttg ctctacgagt ttgccagcct cccccagtgg ctggcttttt tatgtccgta
    22621 acatcctgtg tatcaataaa tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg
    22681 accagatatg ttaaggttgc agctctcttt gaatataatt atcattttca ttacgttatt
    22741 gttacgttta tccggtgcgc cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa
    22801 tatagttatc gtatggtgct caaggagtat tgtgtaatat gaaaataatt atttttagag
    22861 tgctaacttt tttctttgtt atcttttcag ttaatgtggt tgcgaaggaa tttaccttag
    22921 acttctcgac tgcaaagacg tatgtagatt cgctgaatgt cattcgctct gcaataggta
    22981 ctccattaca gactatttca tcaggaggta cgtctttact gatgattgat agtggcacag
    23041 gggataattt gtttgcagtt gatgtcagag ggatagatcc agaggaaggg cggtttaata
    23101 atctacggct tattgttgaa cgaaataatt tatatgtgac aggatttgtt aacaggacaa
    23161 ataatgtttt ttatcgcttt gctgattttt cacatgttac ctttccaggt acaacagcgg
    23221 ttacattgtc tggtgacagt agctatacca cgttacagcg tgttgcaggg atcagtcgta
    23281 cggggatgca gataaatcgc cattcgttga ctacttctta tctggattta atgtcgcata
    23341 gtggaacctc actgacgcag tctgtggcaa gagcgatgtt acggtttgtt actgtgacag
    23401 ctgaagcttt acgttttcgg caaatacaga ggggatttcg tacaacactg gatgatctca
    23461 gtgggcgttc ttatgtaatg actgctgaag atgttgatct tacattgaac tggggaaggt
    23521 tgagtagtgt cctgcctgac tatcatggac aagactctgt tcgtgtagga agaatttctt
    23581 ttggaagcat taatgcaatt ctgggaagcg tggcattaat actgaattgt catcatcatg
    23641 catcgcgagt tgccagaatg gcatctgatg agtttccttc tatgtgtccg gcagatggaa
    23701 gagtccgtgg gattacgcac aataaaatat tgtgggattc atccactctg ggggcaattc
    23761 tgatgcgcag aactattagc agttgagggg gtaaaatgaa aaaaacatta ttaatagctg
    23821 catcgctttc atttttttca gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg
    23881 tggagtatac aaaatataat gatgacgata cctttacagt taaagtgggt gataaagaat
    23941 tatttaccaa cagatggaat cttcagtctc ttcttctcag tgcgcaaatt acggggatga
    24001 ctgtaaccat taaaactaat gcctgtcata atggaggggg attcagcgaa gttatttttc
    24061 gttgactcag aatagctcag tgaaaatagc aggcggagat tcataaatgt taaatacatc
    24121 tcaattcagt cagttgttgc ctgtctgata atagatgtgt tagaaaattt ctgcatggtg
    24181 aatccccctg tgcggagggg cgactggtga acggtatgat ctctttgatg atcgtaagcg
    24241 agaatacgcg ggtttggtgg caccaggccg aactcaccgg gaggcacccg gcaccatgca
    24301 gtattcagag attaggcata tatccaggct cctcatcgca ggagcctttt tacatgcaaa
    24361 aaaaaacgct cctgggaaga gcggctggca agaagaaaca atatgaacaa ttaattaacg
    24421 atgggaataa taccttagag taatcacctt gcgcaactgt aagggcatat ttcatctttg
    24481 cgggctgttt ttctgtgtgg cttctgtgtt tccggagggc agcctgtacc ttttctgact
    24541 cagaacatta tcccggccgg gaggattcat ggcatttaaa cactacgatg tggtcagggc
    24601 ggcatcgccg tcagaccttg ctgaacgaat aactcaaaaa ctgaaggaag ggtggcagcc
    24661 ttatggtagt gcgctgattt cgacagctgg ttatggtgca gagtttatcc agccagttgt
    24721 gagtgagggg gagcttccct cacttgcaga gtcaggcaat cacccgcatg tttcagcgaa
    24781 gcctgaagca gcgccggaat attactatgt gattgcgctt gccggtcagt ccaatagcat
    24841 gtcatacggt gaaggtcttc cgttgccgga gacatatgac cgtccggacc cgcgtattaa
    24901 gcagctggcg cgccgcagta cggtgacacc gggcggtgca gcctgtaagt ataacgacat
    24961 cattccggcg gaccattgtc tgcatgatgt gcaggacatg agccgcctta accatccgaa
    25021 agcggacctg tcaaaggggc agtacggtac cgtggggcag gggctgcata tcgccaaaaa
    25081 actgctgccg tttataccgg cgaatgcggg cattctgctg gttccgtgct gtcgtggtgg
    25141 ctctgcgttc accaccggag ctgatggcac atacagtgac gcgagtggtg cctcggagaa
    25201 ttcaacccgc tggggtgtgg acaagccgct gtataaggac cttatcggtc gaacaaaagc
    25261 agcactgaag aagaacccga aaaatgtgct gtttgccgtg gtgtggatgc agggggaatt
    25321 tgattttggc ggtacgccgg taaatcatgc cgcacagttt ggtgcgctgg ttgataaatt
    25381 ccgtgcagac ctggcggata tggcaggtca gtgcgttggt ggctctgctg gcggtgttcc
    25441 ctggatatgc ggggacacga cgtatttctg gaagcagaag aacgaatcca cgtaccagac
    25501 ggtgtacggc agctataaaa acaaaacgga aaagaatatc catttcgtac cgttcatgac
    25561 ggatgagaac ggggtgaatg tgccgacgaa caaaccggaa gaagacccgg acattccggg
    25621 tatcgggtat tacggttcga aatggcgtga cagctcagcc acctggacgt cacaggacag
    25681 ggcgagccat tttagcacct gggctcgccg ggggattatt tccgaccgtc tggcaacggc
    25741 gattctggtg cacgccggga gaaccgctga attcattacc ggaaaacagc ctgatatggt
    25801 gaggcccacc gtaccttccg gtgaaggtcc ggagagagag gcggaagccc cggccagtaa
    25861 gcgtaccctg atgagtctgc tggcatccgg cgaagacctg gcatcacagg gctggcgcta
    25921 ttatcacaaa ccggcgggcg gagacagtgt taacaaaaac attgctgaag cggtggtcag
    25981 tgatgcgggg gctacgggag gtaaggctct gcaactgaac aaaccggaaa accacatctg
    26041 gtttctggag catgatgcag ccggacaggg ggcggagtta ctgaagaagg gcggacgtgt
    26101 gagcgtacgg tttaagttgc cgggttcact ggtgccgaat cagtttgccc tgggcattta
    26161 ctggcagttg tcgtccctgc cggagggggt gatgctgtca ggagaaggta acgatatgct
    26221 gatgtctttc ttcctgcaga cggatacgac gaacctgaac gcgatgcatc acaggaagcc
    26281 gaatgcgaag ctggatacat tcggggtctt tgataacgga tggcacacgc tggcttttga
    26341 gtttgccgga aacaacagca ttcaggtgac accagtactg gatgagaaac gaggagcggc
    26401 gttcacactg gtgaagtcac cggcatcggg ggcggcggac aaactgcaac tgaccgatat
    26461 atcaaaatcg gcgacgtata cgctgctgat tgacagcatt gcggtggaag tgaacagcac
    26521 agacactgcg gcatgataaa aaaagccgcc agttaccgga atggactggc ggtggtaata
    26581 cccatggaga aatataaagg aacgatactt tcgtgctctg tttttttgaa tgaaaacagt
    26641 tcctattgtc aacaataacg gtaagaaatt atgactttta ttcatcaggt gatgctgtac
    26701 ttctgtacgg cagtctgtgt gctgtatctc ctttcgggtg gataccgggc catgcgtgac
    26761 ttctggcgca gacagattga
//
LOCUS       DAZGTJ010000072        26682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000072 DAZGTJ010000000
VERSION     DAZGTJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.072"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(71..817)
                     /gene="cutC"
                     /locus_tag="ACHK8E_003553"
     CDS             complement(71..817)
                     /gene="cutC"
                     /locus_tag="ACHK8E_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP0345769.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(831..1397)
                     /gene="yecM"
                     /locus_tag="ACHK8E_003554"
     CDS             complement(831..1397)
                     /gene="yecM"
                     /locus_tag="ACHK8E_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0345770.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1613..3346
                     /gene="argS"
                     /locus_tag="ACHK8E_003555"
     CDS             1613..3346
                     /gene="argS"
                     /locus_tag="ACHK8E_003555"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP0345771.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3399..3791)
                     /gene="flhe"
                     /locus_tag="ACHK8E_003556"
     CDS             complement(3399..3791)
                     /gene="flhe"
                     /locus_tag="ACHK8E_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP0345772.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3791..5869)
                     /gene="flhA"
                     /locus_tag="ACHK8E_003557"
     CDS             complement(3791..5869)
                     /gene="flhA"
                     /locus_tag="ACHK8E_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP0345773.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5862..7010)
                     /gene="flhB"
                     /locus_tag="ACHK8E_003558"
     CDS             complement(5862..7010)
                     /gene="flhB"
                     /locus_tag="ACHK8E_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP0345774.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7199..7843)
                     /gene="cheZ"
                     /locus_tag="ACHK8E_003559"
     CDS             complement(7199..7843)
                     /gene="cheZ"
                     /locus_tag="ACHK8E_003559"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP0345775.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7854..8243)
                     /gene="cheY"
                     /locus_tag="ACHK8E_003560"
     CDS             complement(7854..8243)
                     /gene="cheY"
                     /locus_tag="ACHK8E_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP0345776.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8258..9307)
                     /gene="cheB"
                     /locus_tag="ACHK8E_003561"
     CDS             complement(8258..9307)
                     /gene="cheB"
                     /locus_tag="ACHK8E_003561"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP0345777.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9310..10170)
                     /gene="cheR"
                     /locus_tag="ACHK8E_003562"
     CDS             complement(9310..10170)
                     /gene="cheR"
                     /locus_tag="ACHK8E_003562"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP0345778.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10189..11790)
                     /gene="tap"
                     /locus_tag="ACHK8E_003563"
     CDS             complement(10189..11790)
                     /gene="tap"
                     /locus_tag="ACHK8E_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP0345779.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11836..13497)
                     /gene="tar"
                     /locus_tag="ACHK8E_003564"
     CDS             complement(11836..13497)
                     /gene="tar"
                     /locus_tag="ACHK8E_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP0345780.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13642..14145)
                     /gene="cheW"
                     /locus_tag="ACHK8E_003565"
     CDS             complement(13642..14145)
                     /gene="cheW"
                     /locus_tag="ACHK8E_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP0345781.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14166..16130)
                     /gene="cheA"
                     /locus_tag="ACHK8E_003566"
     CDS             complement(14166..16130)
                     /gene="cheA"
                     /locus_tag="ACHK8E_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP0345782.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16135..17061)
                     /gene="motB"
                     /locus_tag="ACHK8E_003567"
     CDS             complement(16135..17061)
                     /gene="motB"
                     /locus_tag="ACHK8E_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP0345783.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17058..17945)
                     /gene="motA"
                     /locus_tag="ACHK8E_003568"
     CDS             complement(17058..17945)
                     /gene="motA"
                     /locus_tag="ACHK8E_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0345784.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18072..18650)
                     /gene="flhC"
                     /locus_tag="ACHK8E_003569"
     CDS             complement(18072..18650)
                     /gene="flhC"
                     /locus_tag="ACHK8E_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP0345785.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18653..19003)
                     /gene="flhD"
                     /locus_tag="ACHK8E_003570"
     CDS             complement(18653..19003)
                     /gene="flhD"
                     /locus_tag="ACHK8E_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP0345786.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            19784..20212
                     /gene="uspC"
                     /locus_tag="ACHK8E_003571"
     CDS             19784..20212
                     /gene="uspC"
                     /locus_tag="ACHK8E_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP0345787.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(20219..21643)
                     /gene="otsA"
                     /locus_tag="ACHK8E_003572"
     CDS             complement(20219..21643)
                     /gene="otsA"
                     /locus_tag="ACHK8E_003572"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP0345788.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(21618..22418)
                     /gene="otsB"
                     /locus_tag="ACHK8E_003573"
     CDS             complement(21618..22418)
                     /gene="otsB"
                     /locus_tag="ACHK8E_003573"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP0345789.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(22585..23571)
                     /gene="araH"
                     /locus_tag="ACHK8E_003574"
     CDS             complement(22585..23571)
                     /gene="araH"
                     /locus_tag="ACHK8E_003574"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP0345790.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(23586..25100)
                     /gene="araG"
                     /locus_tag="ACHK8E_003575"
     CDS             complement(23586..25100)
                     /gene="araG"
                     /locus_tag="ACHK8E_003575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP0345791.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25170..26159)
                     /gene="araF"
                     /locus_tag="ACHK8E_003576"
     CDS             complement(25170..26159)
                     /gene="araF"
                     /locus_tag="ACHK8E_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP0345792.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         6475 a         7299 c         6455 g         6453 t
ORIGIN      
        1 cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca
       61 acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg
      121 ccccgtctac gacataacgc gaatactcgt ccgcgtgtgc atctgatgac atggacaatc
      181 cttgattacg ataacgcatt ggtgaggctt gccacgctcc cgcggagcta tggacttcca
      241 gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct ccggccataa
      301 tgattggagc atcaccatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt
      361 ctgatttttg ccctgatgtc agtacccgag caacgcccaa ttccgcaaga ttattgagtg
      421 tatttaaagg gttagcgcac atatcgaagg cgcgatgaaa agtcacggcc agcggaccgg
      481 cagcagccat tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagaacac
      541 ccgtcaccag tccaggaaaa cctaattcgc ggactgtgcg cacatcctca agaatggcgg
      601 caaactcacc gtcgctgtag caaaaatcac caccgcgtgg gcgaataata ggatgcacag
      661 ggatcgtcac ccgctggcgc acggatttca gcacccccaa cgacggcgtt aagcccccct
      721 cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc gttagcgcgc
      781 attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa ttacgccgac
      841 tgctcgctgg caacgatctc ttcaatggac cacggatgaa atttaatggt cgttttgcca
      901 tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct caccttttgg cgaactggtt
      961 ttaactgaga tccccggcag gctgagtcct tcatcagaaa gcaatgccag cgcacgggca
     1021 ttcagcgttt ctggatcgcc cggcagaacg atttcaatat gttcccaacc ttcgtgcggg
     1081 taacgttttt ccccgggcca cggtagctcc acaatggaaa actgccagtg cgcaacctgt
     1141 accggttcat gcaatttaaa cagacaaatc ggcctgccat tgatcatatt ttctgacaaa
     1201 agctcgccgc actgttcaaa cccgcgacgc cagcgctcag cggtggcgtt ttgatggcaa
     1261 cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca accccaaacg gcgggaaagt
     1321 tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata
     1381 gaatgccagt tcgccataat cactcttcgt cttccactaa aagcgttaat ttaccctgtt
     1441 gccctgcgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg aaaacgacaa
     1501 tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc gccctaattt
     1561 ctttaactgg tgcgggtaat ttttgctcgc ttcatcaatg taaggtattc cggtgaatat
     1621 tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga
     1681 ttgcgaaccg caggttcgtc agtcagcaaa agttcagttc ggcgactatc aagctaacgg
     1741 catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag agcaggtgct
     1801 gactcatctg gatcttaacg gtatcgccag caaagttgag atcgcgggtc ctggctttat
     1861 caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc tggcgtccga
     1921 tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg gttgactact ctgcgccaaa
     1981 cgtggcgaaa gagatgcacg ttggtcacct gcgctctacc attattggtg acgcggcagt
     2041 gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg
     2101 cactcagttc ggtatgttga ttgcgtggct ggaaaagcag cagcaggaaa acgccggtga
     2161 aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt acgacgaaga
     2221 tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg gtgacgaata
     2281 tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc agatcaccta
     2341 cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggtgaaagcc tctacaaccc
     2401 aatgctgccg ggtatcgtgg cggacctgaa agccaaaggt ctggcagtag aaagtgaagg
     2461 cgcgaccgtc gtattccttg atgagttcaa aaacaaggaa ggcgaaccga tgggcgtgat
     2521 cattcagaag aaagatggtg gctatctcta caccaccact gatatcgcct gtgcgaaata
     2581 tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc gtcagcatca
     2641 acacctgatg caggcgtggg ctatcgtccg taaagcaggc tatgtaccgg aatccgtacc
     2701 gctggaacac cacatgttcg gcatgatgct gggcaaagac ggcaaaccgt tcaaaacccg
     2761 cgcgggtggt acagtgaaac tggccgatct gctggatgaa gccctggaac gtgcacgccg
     2821 tctggtggca gaaaagaacc cggatatgcc agccgacgag ctggaaaaac tggctaacgc
     2881 ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca cggactacat
     2941 cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca tgcagtatgc
     3001 atacacgcgt gtattgtccg tgttccgtaa agcagaaatt gacgaagagc aactggctgc
     3061 agctccggtt atcatccgtg aagatcgtga agcgcaactg gcagctcgcc tgctgcagtt
     3121 tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtaatgt gtgcttacct
     3181 gtacgatctg gccggtctgt tctctggttt ctacgagcac tgcccgatcc tcagcgcaga
     3241 aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacggcga agacgctgaa
     3301 actgggtctg gatacgctgg gtattgagac agtagagcgt atgtaatcgc gttatacggc
     3361 aaaagccgat gtcatggcag acatcggctt ttttatcatt aacgataatt cacaatcact
     3421 tcattccgtt gtacctttag cggcggaatt aaccgcccac cgccagacac ttcccagata
     3481 aatcgcaacg gttctatcgc cggtataccg gaaaaggcca cggtggttcc actctgccct
     3541 tctaattcga cacaacgaga ttgtgaacac aagcgaaccc gcagtcctgc aggtgtcggg
     3601 ccgataagct gataacgcca cgccaccagc gtcatcaatc ctgcagcagg ttgtcgagta
     3661 gaaagaggcg ctgacgacat cgactcacca cggtgattca gcgtaacgcc cacactactc
     3721 gcctgccaca tcccctcccc ggcggcttgc accagcagcg gaaacaataa tattgctaat
     3781 aaagttctca tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagata
     3841 gttccagatt cgacagcacc actaactgcg gcaggctgcg gcgcaggaag cgagacaata
     3901 atggtcgcag cgcgtggttc accaacaata ccggcggcgc acccaacatc tcctgacggg
     3961 atagcgcttc ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtccccccc
     4021 cgccctgcaa cgcctgtagc aacaaacgtt ccagcggtgt atcgaggcca ataacatgga
     4081 cttcatcttt gccaggaaac cactgctggg taatcgcccg gcccaacgct acgcgcacga
     4141 cggcggttaa ttcatgcgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa
     4201 tggtgcgcat atcgcgaatc ggcacttttt catcgagcag attttgcagc actttatgca
     4261 gtgtggtaag cgtgacgaca ccaggaacga gatcttccgt cagctttggc atctcctggg
     4321 cgacgcgatc cagcagctgt tgtgcctcct gacgaccaaa cagctctgcg gcatgctggc
     4381 taatgaggtg gttaagatgg gttgccacca ccgtgctggc ctcaaccacg gtgtacccct
     4441 gaatctgcgc ctgttcttta agcgcacttt caatccagat agcattcagg ccaaatgccg
     4501 gatcgacggt cgcctcacca ggtaacgtcc cggcagcagt accagggtta atcgccaacc
     4561 aacgccccgg ataagcatca ccactgccta tctccacgcc tttcatcaaa atgcgatagc
     4621 gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac cggcggcaga aaccccatct
     4681 cctgggcaaa tttcttgcga atactgcgta tacggcccag caactcacca tcctgctgga
     4741 aatcgaccat cgggatcagg cgataaccca cttccattcc cagagaatct tccagttgca
     4801 catcgttcca cgtcgcttcg acaacggtat tattctctgc catttttacc ggttttggtt
     4861 cggcaggcgc tttttgttca cgtccgcgga tccaccaggc cagtcccagc aatccggcag
     4921 tgaacagcaa aaataccagg ttcggcattc caggcaccag gccgagtaaa ccgagcacgg
     4981 cggcgcttaa caacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa
     5041 catcctgatc ggtgctgaca cgcgtaacga tgacccccgc ggcggtagaa atcaccaacg
     5101 ccggaatttg tgccaccaga ccgtcgccaa tagtcaatag cgtataactt tccgccgcgt
     5161 gtcccatgct cataccatgt tgcagcacgc cgaccagcaa cccaccgaca acgtttatga
     5221 ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc ccgtccatcg
     5281 agccgtaaaa atcggcttcc tgagtcactt cggagcggcg ttttttcgct tcatcttcac
     5341 caatcaatcc ggcgttaagg tcggcgtcaa tcgccatctg cttccccggc ataccatcga
     5401 gaacaaagcg cgcgcctact tccgcaatac gcccggcacc tttggtaatg accataaagt
     5461 tgatgatcac gagaatgacg aacaccacga taccgatagc gaaattgcca ccaacgagga
     5521 agtgaccgaa cgcttcgacc acctttccag ccgccgccgc gccggtatgc ccttccatca
     5581 aaatgatacg ggttgaagcc acattaagtg ccagacgcaa caacgtggta aacaacagaa
     5641 tcgtcggaaa cgcagcaaac tcaagcgtgc gttgggtaaa catcgccacc agcaacacca
     5701 tgatcgacaa ggcaatattg aaggtaaaca gcaggtcgag tatgaatgcg ggcagtggca
     5761 gtaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc tgccagtgtg
     5821 tcgatttcag gtttgcgggc aggcgcagca tcgcggccag attactcatg ggtcggtttc
     5881 tcgttaataa aatccagggc ttccggcacc ggaagatgag taggttgtac agggcgctgt
     5941 ccaccagcca gacgccagcg tttcagttgc cagacccagg ccagcacttc cgccaccgcg
     6001 gcgtacagtt gacccgggat ttgttgaccg atctccgcat gtcgatacag cgcacgcgcc
     6061 agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc caatttcacg aatgcgcagc
     6121 gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac
     6181 tgcaaagcca ctgagtagtg agttgggtta ttgacgataa cgtccgcttt cggcacatcg
     6241 gccatcatcc gccgccgcgc cgcagctcgc tgcatctgac ggattcgccc tttgacatga
     6301 gggtcgcctt cgctttgttt gaactcatca cgaatatcct gccgtgacat acgcagcttt
     6361 ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca ccatcggaat gacaccaagc
     6421 accaccagca atgcgcacag ccctaccaga tccatcgcat tacccatggc ggtaatgggg
     6481 gactccgcca ttaagcgcat catttgcggc cagtggtgcc agagataaaa ccccgtcaca
     6541 ctgccgacca ggatggattt caaaatcgct ttaagcaact ccgcaccggt ttgtgccgaa
     6601 aacatccgtt taatgcccgg tagcgggttg agtttggaaa acttcggctg caaggatttg
     6661 ccgctaaata ccaggccacc caacataacc ggagaaataa gcgccaccag caccacaccg
     6721 ctaatcagcg gcagcagcgc cagcatggct tctctgatca gcagaataat ttgcccgagg
     6781 atcagattcg ggtcattgat aatactgtga tcaaaatgca gcccagcgga gagcatgccc
     6841 gacaatcgac gggccagcga cacaccgcca aaccagataa cacacacgcc cactaacaaa
     6901 atcagcagtg acgttaattc acgtgaacgc gggatttgcc cctcttcccg cgctttttct
     6961 agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc
     7021 ctggaaagag tattaagcgt aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa
     7081 ggggcaaaac aggcgggatt tagggctttt actgccacac atcaagcata gtatgcgttt
     7141 gtcggatgcg gcgtcatcgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc
     7201 aaaatccaag actatccaac aaatcgtcca cctgatcctg actggctacc acaccggctt
     7261 tgctggtatc gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact
     7321 cctgttccgg gatgttttcc aacagcacca tcaacaactg gcgttcgatc tcctgaatga
     7381 catccatcat ccgcttaata acctgcccgg tgagatcctg aaaatcctgc gccatcatga
     7441 tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg gacatctgcc agaaattgcc
     7501 gcgtatctgt taccagttct cgggcgtcgg caaggtcaat cggatcggca aaccagtcat
     7561 cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg
     7621 cctcaacact gttcagcgcc cgctccgcag cctgggcagt catctgcaca acatagtaca
     7681 aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca
     7741 gttcccgcaa actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc
     7801 cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg
     7861 cccagtttct caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc
     7921 accacatagc cactggcccc cgcttgcgcc gcagcaataa tgttctcttt cttcgcttct
     7981 gcagtcacca ttaacactgg caatgctgac atcgcgccat ccgcacgaat cgttttcagc
     8041 aattccaggc catccatgtt gggcatgttc cagtcggaga taacaaatcc ataaccgcct
     8101 gcctgcaact tattgagagc gtcgacacca tcttccgctt cctcaacatt attgaatcct
     8161 agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa
     8221 aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg
     8281 cactaatttt tgccagcatt tgctggctta cctggctaag atcgaccact tcgcaaacac
     8341 cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgtg
     8401 caagggtcca tgcccccgcc tgacgcatcg ccaacattcc caccgcgccg tcgttgccca
     8461 taccggtcag gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca
     8521 acacatctac cgaaggccga tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt
     8581 aatttgcgcc actacgcgcc agctccatat gccgatcgcc cggcgcaata taagcatgcc
     8641 ccggcaagac acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa
     8701 gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg ctgggtaatt aacagtgccg
     8761 ggctggaaag cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag
     8821 caccaatcgc aatcagtttt tcagagctca acaacggccc tgccttcagc gttgtcggtg
     8881 ccgacaatgg cttatgtgct gcaaggctcg cctttgctgc tgtacgcacc ttttcagcaa
     8941 tcatttcgct atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat
     9001 ctatcgcccc cagctccaat gcgcgcagcg tgacttctga tcctttgccg gtcagggaag
     9061 aaaccatcac aacgggcatt gggcgcaaac gcattaattt ttcgagaaaa tccagtccgt
     9121 ccatccgcgg catttcaaca tccagcgtta gcacatccgg attgaatttc ttaatcaaat
     9181 cacgcgcaac cagcggatca ggggcggtcg ccaccatttc catgtcgcta tggctgttga
     9241 tgatttctgt catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt
     9301 tgctcatcgt taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg
     9361 ctcaaggtgg ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag
     9421 cggaacaaag cggcgcaaaa tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt
     9481 acgacagaag atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtaaattcag
     9541 cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg
     9601 tcccgtccct cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc
     9661 atggcgataa ataccgcttc tggctttttc cagcacttcg gtgtcgatat cgctggcaaa
     9721 cactttccag cgtccgggca cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta
     9781 cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg
     9841 tgcgtgatcc gcgagcagag gaaaatgatg cgcctcacgg aaaaatgccg tcagattcgt
     9901 ggtcagcgaa ttgataaacg cctgccactc accgctgtgc agattagatt ccagcaagtt
     9961 caggtaatga ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta
    10021 aaccatgtcg cgtttatggt cggccaggac gatcccggct cgttgataga tcaattgact
    10081 tatccgccga aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga
    10141 cgtttgccca cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac
    10201 tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact tcatgctctt caagggtaaa
    10261 taccgccacg cgcgacgaaa gatggtcggc ctgattcgcc agttgttctg ttgccaccgc
    10321 cgcttcttct accagggagg cgttctgctg tgtcacctga tccatctggc tgacagcctg
    10381 tgcaacctgc tcaatccccc gccgttgttc ttccgaggcc gaagcaattt ctcccataat
    10441 gtcgttcacg cgggtcaccg aactgacaat atcagtcatg gtcgcggcgg cgttattcac
    10501 cagtttcgaa ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt
    10561 tgctgcctgg gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc
    10621 acgcccctgc tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt
    10681 ctggaaagca atgccgtcga taacgctgat aatgtcgcca attttttgcg agctggtagc
    10741 gatctcctgc atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt
    10801 cgcggcattt tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt
    10861 ggcggttaat tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt
    10921 gcgacttgag agatcgttat tgcctgcgac aatctccgcg ataccaatgt gcatctcctg
    10981 gcttccctta cgcacatcac ttaccgtccc acgcaaagcc tgctgcatgg tcttcaggct
    11041 ggcaaaaatg gcggtgatct cattgcgacc atacaccgca atcggacgtg ccagattacc
    11101 cgcagcaatg ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg
    11161 cgtccaccac agcgcactgc tgatgtagat cgccgcaaca ataatcatgc tgataaacac
    11221 cagcgccgaa atgtgatagt tacgctggct ttgcgcactg gcactttcca gcacatggtt
    11281 gatctccagc tgccaggcct caaagttaac gtcaaacgca ttctgtgatt cctgcaccgg
    11341 cgcagtgagg aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc
    11401 gttgtgccag cgggcaaaac ttttctccgt ctctttttgc aacgccctga cgtgctcgtt
    11461 gcccgcagtc atcgccataa aacttttaaa cagcgtggtg gattgcgtca gactggcgcg
    11521 cgccgtcgtc atcaacgttt taatgtcatc cgccggatag ctaagcgccg tcagagtgcc
    11581 cgctttgttc agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc
    11641 acgttgctga ttgctctgct cgacctgatt cagtcgttgc aaatcgtcgc gaaatgccca
    11701 aaaagacatg ccgttactgc caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag
    11761 cgtggtcgaa attcgaatac gattaaacat caacgctccc catcaggcgg caatgaccgc
    11821 gttagtaaat actcgtcaaa atgtttccca gtttggatct tgttcagcaa ttcgcagtcg
    11881 tggctgtgcc ggtggttgct cactggcagg acgggatggt gtttgcggtt tattggtgag
    11941 tgggctggct gccagacgga acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc
    12001 cagcgcagct gcggcggcag ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg
    12061 atccatttcc gaaaccgcca atgcgacttg atcgatgcca cggctctgtt catccgatgc
    12121 cgatgcaatc tcgcccataa tgtcagtcac gcgggtgaca gcattgacga tattgttcat
    12181 tgtttccccg gcgctttcga ccagcaccga accggtatca acgcgcgaga cggagtcttc
    12241 aatgagggct ttgatctctt ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc
    12301 gcccgccacc acggcaaaac cacggccctg ttcacccgct cgcgcggctt caacagcggc
    12361 attcagcgcg agtatattag tctggaaggc aataccatcg ataacgctga taatgtcggc
    12421 aattttcttc gaactatcgg cgatctcatg catcgttttc actacgccat ccaccacttt
    12481 gccgccgtgc tgggcggtgt cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt
    12541 atcggcgttt tgcttcactg tcgcggtgag ctgctccatg ctggcggcag tttcttccag
    12601 cgcggatgcc tgctgttccg tacgggagga aagatcggtg ttgcccgccg caatttcacg
    12661 ggtaccggca tagatggcat ctgaaccttc acgcacatga gtgacggtgt cagtcaaaga
    12721 gcgttgcata tgtgaaacgc tctgcgccag gtcgcccatt tcactgcgcc cgtcaatggt
    12781 cagggtattc gccaggttac caccggcgat ttcgcgaatg tgagcaataa tttttgccag
    12841 cggtgtaagc aacatacggc gaatgccgta ccacgccacc agcagaatca ataccaccac
    12901 cagcgcgata accgccagtt gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac
    12961 gatatcgcga tacagttttt cactgctgag ggcgtactga gcaaacgctt cgcccattgc
    13021 attttgcatt ccctgggttg gctgagcgaa ataagctcca gtattgccat aatcaagata
    13081 atcaatcagt tcagttaacg ctgtgtgata gtttttatat ttttcatcaa tattacgact
    13141 ggtagcgacc atttcaggta acggtgccat gcttttgaat tttttataat gagtcgctgc
    13201 ctgggccaat gttttcctgg cgctatcgag caattcaact ttggcattac tttgctgatt
    13261 ggatgaatcc atcatcatcc gcaccgctga acgactcagg ttaatgcgcg tttgcagcat
    13321 taaatcccat gttgacgtca gctcgccctg ctgttcccgt aattgattgg aaaccacaaa
    13381 gctcttctgg ctatggtgaa gggaagaaaa aaacagactg ccggaaataa gctgtaacag
    13441 tgcgaatacc cccagcacca ttaccaacag cgtgactacg cggatacggt taatcataag
    13501 gcaccttcct gaaaacaagt tgatctcgtt atcggcaagg aagggggaaa ctttattgct
    13561 gatgccaccc gccgcgaaac tgaaataaaa aacccgatgc gcagatcatc gggttcattg
    13621 caattgagga aatcgggaga attacgccac ttctgacgcc gcgctatcta atagcgccat
    13681 ctcttcgctg ttcagcagtt tttcgatgtt caccagaatc aacatacggt cgcccagtgc
    13741 acccagtcca gtgagatatt ctgttgaaag cgtcacggca aattccggtg ccggacgaat
    13801 ttgctccgcc gtcaatgaaa gcacgtctga gacgccgtca accacgatgc cgaccacccg
    13861 ctgtccgaga ttcaggacga taactaccgt gttgtcgtta tagtccacat ccacctggct
    13921 gaacttaatt cgtaagtcca caatcggcac aataacaccg cgcagattcg tgacgccttt
    13981 gataaacgct ggcgtgttcg caatccgtgt tacctgatcg tagccacgga tctcctgcac
    14041 tttcagaata tcaataccgt actcttcatc accaagggta aataccagaa attcctggcc
    14101 tgacggctcg ctggccagct ttgttacatt cgtcataccg gtcatattgt taccttttta
    14161 ctcattcagg cggcggtgtt cgccatacgt tgttcgcggt ttatcgcctg caaggcggag
    14221 acatcaacaa tcagtgccac gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg
    14281 actttgcgat agttactttc aaggttttta accacaacct ggtgttgacc aattaattga
    14341 tccaccagca aggcgtagcg gcgaccgcca ctttgtaaga tcaccacaat tccctgggta
    14401 gcttcggttt tcgcgcccgc gacgttgaac actttccaca gttcgacgat gggcagatat
    14461 tcaccccgca cttccagcac ccgctcgccg ccagccagtg gatgcagatc ggcttcacgg
    14521 ggttgcagtg attccatgac agcattcagc ggcagaatga aaacttcatc cgcaacgcgt
    14581 acggacatgc cgtcgaggat ggccagcgtc agcggcagta aaatgcggat cgtcgtgcca
    14641 gtaccctgct tcgactggat ttcgacatga ccgcccatct cctggatatt acgtttaacg
    14701 acgtccatgc cgacgccgcg cccggagacg tcggtgacct gctccgccgt ggagaagcca
    14761 ggggcaaata tcagcatcgc gacttcgtcg tcgctcatgt tttcgctgac ggtcagacct
    14821 tgcgaggccg cttttgccag aattcgctca cggtttagcc ccgcaccatc gtcggtcact
    14881 tcaatgcaaa tgttgccgcc ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt
    14941 ttacctgcgg cgagccgttt ttctggcagt tcaataccgt gatcgaggct attgcgtacc
    15001 aggtgggtca gcgggtcgat aatgcgttct atcaggctct tgtcgagttc agtggaactg
    15061 cccaccagcg tcagttctac ctgcttgccg agttttcccg caagatcacg caccagccgg
    15121 ggatagcgac taaagacata ctccatcggc atcatgcgaa tcgacatcac tgattcctgc
    15181 aaatcacggg cgttacgttg taactgcccc atgctggtaa tcaaatcacc atgattaacc
    15241 gggtccagtt cactagaacg ctgggcaagc attgactggg tgatgaccag ctcgccgacg
    15301 aggttaatta attgatcaac cttttctacc gctacacgga tgctggtgga ttcactgctg
    15361 cgcgtcgttt tttcccgctc cacgcggccg gttggcgctt gttcggctgc cagtttaagc
    15421 actggtgggg gggatatttt gggcgagact tctactgttt caaaggtaat ctgatcggct
    15481 tcaatcacaa aacagagtac cgctgtgatg tcatcttcgg cgatgtcgcc cggtaatatt
    15541 gccgagagag aatccgcccc tttcaccaca tcagttaacg ttgtcagatg ccccagctct
    15601 tcttccagca ggtcgacttc cccggccttc aggcgcgaaa ggataattcg tcgcggcgac
    15661 tgatcgcggc tctgctcatc ttgcggttca cttttggcaa ccacacttaa tcgggtcact
    15721 gcagatggcg tttcgccttt cgcttctaat gccagttgac gcaaggcctg gcagatataa
    15781 tcgaagctgg cggcatccgg ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg
    15841 atgtccttcg tttccaaaaa cagattgata atgtcggtgt tgagttgcat ctcacctcgt
    15901 ctggcttcgt cgagcaggtt ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca
    15961 aaggttcctg cccctccttt gatcgagtgg gcagcccgaa agatggcatt caattgttcg
    16021 gcatctggcg cttccggctg caaaaccagc agatgctgct ccatgtcagc caacagttcg
    16081 tccgcttcat caaaaaatgt ctgataaaaa tcgcttatat ccatgctcac gctgtcacct
    16141 cggttcggct gatggcattg tgggaacact gacctgtggt gcaacctcag gtttttccag
    16201 ggcgcttact ggctcattct ggctttcggc gttttcatgc aaaatggcct gttcggcttg
    16261 tttgttcagt accagcaggc tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct
    16321 taagcgcatc gttgccgcca tgccgacgac acgtaacact ttgccgccat tcaacccacc
    16381 aaccatcagt tcgcggcggg atgcattggc acgatcggca gaaagctccc agttgctgta
    16441 acctttctca ccgctggcgt agggaaaatc atcggtatga cccgagaggc taattcggtt
    16501 gggaataccg ttcagtacgg gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac
    16561 gtcggcactg ccagttctaa acatcgggcg attctggcta tcgatgatct gaatacgtag
    16621 accttcctgg accagatcga ttttgagatg tggacgtaac gcccgcagtt tcggatcgga
    16681 ctcaatcaac tggtcgagat cgccacgcaa tttccgcaat cgactttgct ccatgcgttt
    16741 tttcagctct tcgatgttcg gctgcttatt cacttccccc tggctttggg tgtaatcatc
    16801 acccccaccg ggaattgggc tttcactatt agaaatgcga tcgccgcccg taaccgcagt
    16861 cgccagtgga gtccggaagt actccgcaat ctgaatcagc tcttttgggc tggagatgga
    16921 gattaaccac atcaccagaa aaaaggccat catcgcggtc ataaagtcgg cataagcaat
    16981 cttccacgat ccatgtgctg ccccgtggct tttggctttg cgtcgtttga cgacaataat
    17041 cggatgcgct tgattcttca tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc
    17101 gcacgcacat gctcttccag ttcaataaac gacggacgtt cgctggaata gagcgttttg
    17161 cgaccaaact caacggcgat aggcggtgcg taaccgttca gattagatag cagtgtgact
    17221 ttgacgcact gcatcatttt gctggtttcg gcgcttttct gacgtaaaac actcgctaat
    17281 ggggaaataa atccgtaagc caataaaata ccgaggaaag tccccaccat cgcatgtgcg
    17341 ataagcgccc ccagctcggc ggcaggacga tcggctgaac ctaacgcgtg aacgaccccc
    17401 attacagccg caacaatacc aaacgccgga agtgagtcac cgaccagcgc cagactgttc
    17461 gccgggactt ctgcctcgct ttcgtgcgtc tcaatctctt catccatcag agcttcgatt
    17521 tcgaaggtgt tcatgtgacc gctgataatc aggcgcagat aatcgacgat aaattcaagc
    17581 atgacgctat ccgcaaggat gcgtgggtag ctggcgaaga tctcgctctc acggggattt
    17641 tcaatatcac gttccagcga aaacatcccc atctgccgcg atttcgccat caaccgataa
    17701 agcagagcca gcagatccat atacattgct ttggtgtatt tggagcgacg aaacagcaac
    17761 ggcagcgcct tcagcgtgcc tttaatcgct ttgccattat tgccgacgat aaacgaccca
    17821 atccctgcac cggcaataat caccagttca gcgggttgat agagtgctcc aaggcttcca
    17881 ccggtcatca aataaccgcc gaaaactgta ccgagaacaa ccaggtaacc taataagata
    17941 agcacgacat catccttcca ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac
    18001 acgcgatagg ggcaaaaaaa agcagcggta cgtcgttacc gctgctggaa tgtttcgcca
    18061 caccgtatca gttaaacagc ctgtactctc tgttcatcca gcagttgtgg gataatatcg
    18121 gcaggattct gggaaagttt acgtcttttt actgcccggg atggcggctg acataagctg
    18181 caggcaaagc tgccaacagg ctggtgagcg tgggtaataa aattgccgcc gcagcagttg
    18241 cagctggaaa gttgcagtaa tccactttca acaaaccgca ccaatgtcca ggcacgggtt
    18301 aatgccagca gcggtccctc ttctgcttgc gggcactgtt caaggtataa acggtaggct
    18361 ttgatcaccg catcgacgcc attacacaaa ccggttttca gtaaaaactg ccatgcgtta
    18421 cagaacatcg aagcatgaac gttttgttcc caggtcataa accagtcggt tgagaatggc
    18481 agcatgcctt tcggcggtgg gcttccgcgc agttctttat agagttttat caggcgtccg
    18541 cgacttaact gtgtttcgct ttccagcatc tgcaaacgag cgcccagggt gatcaattcc
    18601 attgccagct gaatatcccg cgcttcctga acaatgcttt tttcactcat gatcaggccc
    18661 ttttcttgcg cagcgcttct tcaggctgat taacatcatt cagcaagcgt gttgagagca
    18721 tgatgccggt atgaatttgc tggagatcgt caacgcggga atcttgcgtc aactgagtaa
    18781 tcgtctggtg actgtcaaaa cggaagtgac aaaccagttg attggtttct gccagcttaa
    18841 ccatttgcgg aagagtcagt gccgctaacg ttgtcgccat ttcttcattt atgccgagac
    18901 gaaacatagc ggacgctttg tcctgaacaa tcagacgctg tgcaagtagt aaatatgaca
    18961 agttgatgtc ataaatgtgt ttcagcaact cggaggtatg cattattccc acccagaata
    19021 accaacttta tttttatgcg gtctcaccgc accccgtgat gtcgccggga agccccggta
    19081 aaaaataatt agcattagaa tagttgcgat aagctgcaat aggcagagcc cctttgtggg
    19141 atttaataag tccttacaaa tagaaggggt tctttacact tatctaagat ttttcctaaa
    19201 tcgacgcaac tttactcgtc actacacgca catacaacgg aggggggctg cgattttcaa
    19261 taatgtgtga tgcagatcac acaaaacgct caattactta acataaatgt ataagtcata
    19321 cttttgtttt gggtgtattt caatctgtta aaaagttttt cgcgacgcta gcaagcaaaa
    19381 atgaaacagg aataatagaa atgggatgtt gcgcacagtt aaaataactc accgtaaata
    19441 atcatctgct ataaataatt gctttaatgc aataccagat aagctatttt taaacagaca
    19501 cttaccgcac aacaaactaa ttaaatacaa agaaaggcaa caatcaactt aatgttaata
    19561 aaatgtttta catatattaa ccacttgttt aatatggaga atcagtacat tattttctta
    19621 taattacagt tgaaatcata tgatcaccga gagtgattga ttaattgtat ttactaataa
    19681 tcacgctaat atttacctgt ttgactgagt tgggattgcg tcgtttctcc attaggagta
    19741 aagctttaat gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat
    19801 tcttgtcgct gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat
    19861 cgccaggcca gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta
    19921 caatcaatta gctgcgccga tgcttgaaga tttacgcagc gtgatgcagg aagaaacgca
    19981 aagctttctt gataagttaa ttcaggatgc ggggtatccc gttgacaaga cgtttattgc
    20041 ctacggtgag ttaagcgaac atattctgga agtttgtcgc aaatatcatt tcgatttggt
    20101 gatttgcggt aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt
    20161 gattgcctca agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct
    20221 acgcaagctt aggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa
    20281 ctatctgctt taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga
    20341 taacgtccag catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac
    20401 gatccagcgc agctgcaact tcgtcacgat cgtagggatt aacaattaac gccgacgtta
    20461 actcgtttgc tgctcccgca aattgcgaaa gaacaagtac gcccgggttg gctgggtcct
    20521 gagcggcaac atactctttt gccaccaggt tcatcccgtc acgcagtggc gtcactaagc
    20581 ccacgtcaga gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat
    20641 aataaagcgg cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt
    20701 cgagctgatg acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct
    20761 gggtataacg aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga
    20821 gaaaacgctc cggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta
    20881 cgtttttcag ttccgcttta agttgcgcca gttttggcgg cagtggtccg gcagcctgtt
    20941 tcgctatttc tttcggttca atgccgatcg ggtaaacttc cgttcgaaac gctttgccgc
    21001 aggccgtatg gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa aggcaatcca
    21061 ggaacgccag acgatcgttc tccgtctgga aacccagcaa atcataatca caaagctgtt
    21121 caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc cggcgtcggg aaaggaatat
    21181 gcagaaagaa accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaagggca
    21241 acaggtgata atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat
    21301 ctgccagcaa cgcatttacg cgtagatagc catcccaggc aggacgctga aattgcacca
    21361 gatcgagccg ataatgaaaa gcgggccaga gaacggcatt ggagaatttg ttgtagtatt
    21421 cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt aatgttaccc tttttcacct
    21481 tttttagcgg ctgatcctca ttccctgttt caccgctcca gccaaaccac agtccacctg
    21541 cggctttcag tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt
    21601 ctggtggtgc aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta
    21661 ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc
    21721 acgcccgcca gtcgccatga tgcctgagtt gcgcctgtgc caatttttac tgacattccg
    21781 cccagccggt taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaataca
    21841 ggcgttcgcc cgataaaggg agcttcctgc ataaaggcag caatcgcctc acctttactg
    21901 gtgcctcgcg gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag
    21961 atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga
    22021 taatgcagcg caaaggccat ccctttcgtc tccagctccg caccgggaaa ctgggcgata
    22081 gccgtatgca gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgc
    22141 gttttaccat tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa
    22201 ggttttgcca gtgcgtcaag ctccaccatt gagcgccctg aaatcaatgc caatgcacca
    22261 tcacttgcgg ttgccagtag ctgaagtcct tgcagaatag tgtcaggcac gacgacctga
    22321 tcgggatgcg gtttgatttc cgccagcgtt ccatcgagat caaaaaacca ggcatatttc
    22381 gcggatagtt caggggtttc ggttaacggt tctgtcaccc ggttctcctc cttctctttt
    22441 cttattgtgt tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg
    22501 tcgccatttg caacgaagaa cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac
    22561 gctaaattgt tgtggaaaaa agcatcagac agtgcgtttc gctttttgct tgtaacggtc
    22621 gaaaatcacc gctgccagca ggattaagcc gcgaactacg tactgtgcga aaggagaaat
    22681 attaagcagg ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata
    22741 tgagattttt ccgatgccgc ctttcagaga aacgccacct aaaacgcagg cggagatcac
    22801 aatcagctca tagccaatcg acgtcattgg ctgcccactg gtcatacgtg aagccagaat
    22861 aattccggct atcgctgata ccaggccaga gagaacaaag ataataattt tggtgcgaac
    22921 aaccggtaca cccgccagac gcgcggcctc ttcgttcccg ccaatcgcca gggtattacg
    22981 cccaaaggtg gttttattca gcagcaagcc aaagatgatc agacacgcga cggtgagcca
    23041 gattggcgca ggcagaccga accagttggc gtaaccaagg gcaaagaagc tttcatcttc
    23101 gataccgacc gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt
    23161 tgccagtgtc gtgatcaggg catttatttt cagtttggca ataacaaagc cattgaccag
    23221 gccacagaga acgcccagca acaaacccgc tgccacgcca atccacaggc tttcagtcag
    23281 gttaataacc accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc
    23341 aaagtcaccg gaagcaaggc agaacaacat gccacaagcc accatccccg acatggaaat
    23401 tgccagaccc aaccctttca tattaataaa ggtggcaaag ttcgggacaa aaatggcaca
    23461 ggcgataaaa aggaccgcaa acaccaccag catgccgtac tgatcccaga tacgccctaa
    23521 gctgaatgac gacttaggtg cgccagaacc cgatgtagaa acagaagaca tcatactctc
    23581 cttactcagg caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca
    23641 tctgcctgct cgtgtaacaa ttcaccggcg atttcgcctt cccgcatcac cacaatccgg
    23701 tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc
    23761 gccgccagcg cataaattac gttatatatt tcgtgcttag caccaacatc aatgccgcgc
    23821 gtaggttcat ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct
    23881 ttttgctgat ttccgcctga gagattcatg atcagttgat cagcgcccgg cgttttgatg
    23941 ttgagcgaac gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa
    24001 ccgccgagca catgtgtacg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg
    24061 ggaataatgc cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg
    24121 tggctcggtt tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt
    24181 tgcgtaccgc caaacatgcc tttcattaat tcgctacgcc ctgccccgac cagaccaaac
    24241 agaccgacaa tttctccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct
    24301 ttcacagcat caagacgcag ccgctcctcg ccataactac gcggttgcca accgtagata
    24361 tcgccgatgt cgcgcccgac catcgcctgc accagggcgt cgtggtcaac ctgctgcata
    24421 tcggtaaagg ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca
    24481 aatatttctt ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca
    24541 cgaataacgc ggaaaagatt gtcgatttca cgggcagaga gggagctggt tggctcatca
    24601 aaggcgataa ttttggcatt acgcgccagg gctttggcaa tttcaaccat ctgccactga
    24661 ccaatggaga gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt
    24721 tgtaaacccg cctcataatt cagcaatgag cgattgacaa tgccgccttt atgcggcagc
    24781 tggccgagat agatgttttc cgcgacggtc atttccggca cgagatgcag ctcctggtaa
    24841 ataatcgcca cccccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca
    24901 ttaatcacta cagaacccgt ggttggcgca tagtttccgc tgaggatttt taagagagtt
    24961 gattttcctg cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa
    25021 ctaatatccg tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga
    25081 tacggggtag actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc
    25141 atcatgcaga ggggaatctt tccggcaaat tacttaccgc ctaaaccttt tttctccagt
    25201 tcttctttaa agttgtcacg cgtgatcagc accacgtcgg taacttcggt aaattttggc
    25261 ggttcaacgt cttttgctac ccagttgtaa agcatttcgc tggacttata accatgtacg
    25321 tccgggcttg gcagcaggga accgtagaag ccggttgcct gtgccttaga cagttcgctc
    25381 accgcatcca caccgttaat accgataccg atgatatcgg ccgctttaaa gccctgacct
    25441 tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt
    25501 ttaacttccg gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg
    25561 ttagatttgg taggtacctg ataaatttgt ttttccggga atcctgcagc tttcagtgcg
    25621 tccatagaac ccgttgtacg gcggcgggcg gtatccagtt cgttggcggt aatcgccatc
    25681 accgcgcttt ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctggccc
    25741 tgacgttcgc caattttggt cgcagccatc atcaccagcg gaacggtatc cattggctta
    25801 cctttggcgt taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct
    25861 ttcgccacga tggcagagcc gagtttcggg tccggagtac aaataacgaa acctttcgcg
    25921 ccactggcag ccaggctgtc gatagcgttc aatgtttttt cgccatccgg cacggcaatc
    25981 ttaataactt caaaccctaa atctttcccg gctttatcgg caaacttcca ttcggtctgg
    26041 aaccacggct cttccggttg cttcaccagg aaaccgagct tcaggttctc cgccatagcg
    26101 gattgtgaca taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg
    26161 gttctctcca gctttagtgt cgttttgtgt agggcaaaaa cgaatgacat tcgttaaatt
    26221 aatcggaaaa caaagcatta ccttttaact aaaagataag tgactgtgtt gacatagttt
    26281 tagcgagaaa ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac
    26341 gtgcataaat ttagcgggaa aagacataag gaaaagcgaa tttgtcagac aaattatcga
    26401 gtgcacagca gaataatcca taagattagc ctggaagtcc ttgttgtctt tggtacccat
    26461 gcgggatgtc ttctttttaa ccagtcaata ggccgcatta cctggcgttg agtttttgca
    26521 atggtgtaat aaccacaact caaagatgtg gaaaatgtgc gtcattcatt tcgccattaa
    26581 ttatcactgt gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga
    26641 aaaaacgttt tataaaacca tcacttcatc atgaattcaa at
//
LOCUS       DAZGTJ010000073        26385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000073 DAZGTJ010000000
VERSION     DAZGTJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.073"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            32..598
                     /gene="yceJ"
                     /locus_tag="ACHK8E_003577"
     CDS             32..598
                     /gene="yceJ"
                     /locus_tag="ACHK8E_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0345793.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            602..1177
                     /gene="yceI"
                     /locus_tag="ACHK8E_003578"
     CDS             602..1177
                     /gene="yceI"
                     /locus_tag="ACHK8E_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP0345794.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1219..2271)
                     /gene="trhO"
                     /locus_tag="ACHK8E_003579"
     CDS             complement(1219..2271)
                     /gene="trhO"
                     /locus_tag="ACHK8E_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP0345795.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2496..3416
                     /gene="lpxL"
                     /locus_tag="ACHK8E_003580"
     CDS             2496..3416
                     /gene="lpxL"
                     /locus_tag="ACHK8E_003580"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP0345796.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3588..4814
                     /gene="mdtG"
                     /locus_tag="ACHK8E_003581"
     CDS             3588..4814
                     /gene="mdtG"
                     /locus_tag="ACHK8E_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP0345797.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4897..5271
                     /gene="msyB"
                     /locus_tag="ACHK8E_003582"
     CDS             4897..5271
                     /gene="msyB"
                     /locus_tag="ACHK8E_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0345798.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5272..5499)
                     /gene="yceK"
                     /locus_tag="ACHK8E_003583"
     CDS             complement(5272..5499)
                     /gene="yceK"
                     /locus_tag="ACHK8E_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0345799.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5671..8214)
                     /gene="mdoH"
                     /locus_tag="ACHK8E_003584"
     CDS             complement(5671..8214)
                     /gene="mdoH"
                     /locus_tag="ACHK8E_003584"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP0345800.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8207..9742)
                     /gene="mdoG"
                     /locus_tag="ACHK8E_003585"
     CDS             complement(8207..9742)
                     /gene="mdoG"
                     /locus_tag="ACHK8E_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP0345801.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10136..11293
                     /gene="mdoC"
                     /locus_tag="ACHK8E_003586"
     CDS             10136..11293
                     /gene="mdoC"
                     /locus_tag="ACHK8E_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP0345802.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11301..12782)
                     /gene="clsC"
                     /locus_tag="ACHK8E_003587"
     CDS             complement(11301..12782)
                     /gene="clsC"
                     /locus_tag="ACHK8E_003587"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP0345803.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12724..13257)
                     /gene="ymdB"
                     /locus_tag="ACHK8E_003588"
     CDS             complement(12724..13257)
                     /gene="ymdB"
                     /locus_tag="ACHK8E_003588"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP0345804.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13352..13663)
                     /gene="ymdA"
                     /locus_tag="ACHK8E_003589"
     CDS             complement(13352..13663)
                     /gene="ymdA"
                     /locus_tag="ACHK8E_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP0345805.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13784..14116)
                     /gene="csgC"
                     /locus_tag="ACHK8E_003590"
     CDS             complement(13784..14116)
                     /gene="csgC"
                     /locus_tag="ACHK8E_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP0345806.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14175..14630)
                     /gene="csgA"
                     /locus_tag="ACHK8E_003591"
     CDS             complement(14175..14630)
                     /gene="csgA"
                     /locus_tag="ACHK8E_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP0345807.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14671..15126)
                     /gene="csgB"
                     /locus_tag="ACHK8E_003592"
     CDS             complement(14671..15126)
                     /gene="csgB"
                     /locus_tag="ACHK8E_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP0345808.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            15880..16530
                     /gene="csgD"
                     /locus_tag="ACHK8E_003593"
     CDS             15880..16530
                     /gene="csgD"
                     /locus_tag="ACHK8E_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP0345809.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            16535..16924
                     /gene="csgE"
                     /locus_tag="ACHK8E_003594"
     CDS             16535..16924
                     /gene="csgE"
                     /locus_tag="ACHK8E_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP0345810.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            16949..17365
                     /gene="csgF"
                     /locus_tag="ACHK8E_003595"
     CDS             16949..17365
                     /gene="csgF"
                     /locus_tag="ACHK8E_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP0345811.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            17392..18225
                     /gene="csgG"
                     /locus_tag="ACHK8E_003596"
     CDS             17392..18225
                     /gene="csgG"
                     /locus_tag="ACHK8E_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP0345812.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(18289..18780)
                     /gene="ycdZ"
                     /locus_tag="ACHK8E_003597"
     CDS             complement(18289..18780)
                     /gene="ycdZ"
                     /locus_tag="ACHK8E_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0345813.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(18882..19436)
                     /gene="ycdY"
                     /locus_tag="ACHK8E_003598"
     CDS             complement(18882..19436)
                     /gene="ycdY"
                     /locus_tag="ACHK8E_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP0345814.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(19460..20197)
                     /gene="ycdX"
                     /locus_tag="ACHK8E_003599"
     CDS             complement(19460..20197)
                     /gene="ycdX"
                     /locus_tag="ACHK8E_003599"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP0345815.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(20252..21190)
                     /gene="ghrA"
                     /locus_tag="ACHK8E_003600"
     CDS             complement(20252..21190)
                     /gene="ghrA"
                     /locus_tag="ACHK8E_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP0345816.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            21423..21510
                     /locus_tag="ACHK8E_003601"
     tRNA            21423..21510
                     /locus_tag="ACHK8E_003601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21457..21459,aa:Ser,seq:gga)
     gene            complement(21660..22502)
                     /locus_tag="ACHK8E_003602"
     CDS             complement(21660..22502)
                     /locus_tag="ACHK8E_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0345817.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITCNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDQWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(22587..22784)
                     /locus_tag="ACHK8E_003603"
     CDS             complement(22587..22784)
                     /locus_tag="ACHK8E_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0345818.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(22796..23284)
                     /locus_tag="ACHK8E_003604"
     CDS             complement(22796..23284)
                     /locus_tag="ACHK8E_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0345819.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            complement(23281..23655)
                     /locus_tag="ACHK8E_003605"
     CDS             complement(23281..23655)
                     /locus_tag="ACHK8E_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0345820.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(23745..24098)
                     /locus_tag="ACHK8E_003606"
     CDS             complement(23745..24098)
                     /locus_tag="ACHK8E_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0345821.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            complement(24174..24395)
                     /locus_tag="ACHK8E_003607"
     CDS             complement(24174..24395)
                     /locus_tag="ACHK8E_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0345822.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(24464..24940)
                     /locus_tag="ACHK8E_003608"
     CDS             complement(24464..24940)
                     /locus_tag="ACHK8E_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0345823.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(24956..25441)
                     /locus_tag="ACHK8E_003609"
     CDS             complement(24956..25441)
                     /locus_tag="ACHK8E_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0345824.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(25532..26350)
                     /locus_tag="ACHK8E_003610"
     CDS             complement(25532..26350)
                     /locus_tag="ACHK8E_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0345825.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         6622 a         6667 c         6359 g         6737 t
ORIGIN      
        1 tttctctcgc aaagtcgttt tggatgcact tatgtcattc acaaataccc ctgaacgcta
       61 tggtgttata tctgcggcct tccattggct aagcgcaatt attgtctacg gcatgtttgc
      121 tttaggtctg tggatggtca cgctcagtta ttacgatggc tggtatcacc aggcacccga
      181 actgcataaa agcatcggta ttttgttaat gatggggctg gttattcgcg ttctgtggcg
      241 cgtcatatct ccaccgccgg gtccgctgca gagttattcg cccatgactc gtcttggcgc
      301 aaaggctgga catctcgcat tgtacttatt gctgttcgcc atcggcatca gcggctatct
      361 gatctcaact gccgatggta aaccgatcag cgtttttggc tggtttgacg tccccgcgac
      421 cctcgccgac gctggcgcac aggctgactt tgccggtgcc ctgcattttt ggctcgcgtg
      481 gagcgtcgta gtactgtccg ttatgcacgg atttatggcc ctgaagcatc atttcatcga
      541 taaagacgac actctgaagc gcatgctggg aaagtcgtca tctgactatg gagtataaaa
      601 aatgaaaaaa agcctgcttg gtttaacctt cgcttccctg atgttctctg ccggttcagc
      661 ggttgccgcc gattacaaaa ttgacaaaga aggtcagcac gcctttgtta acttccgcat
      721 acagcacctt ggctatagct ggttatacgg cacctttaaa gatttcgacg gtacttttac
      781 ctttgatgaa aaaaatccag ctgccgataa agtgaatgtg acaattaaca ccaccagcgt
      841 cgatactaat cacgccgaac gcgataaaca tcttcgcagt gcagatttcc tcaatactgc
      901 aaaatatcca caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga
      961 tattaccggc gatctgacgc tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt
     1021 aattggtcag ggcgacgacc catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa
     1081 aattaagctc aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga
     1141 tctgattatt tcagtggaag gcgtacagca gaagtaagag tgaaatgttg ccggatggca
     1201 cgcatccggc aatgatattt attctgttgg atcaggaatg cccagtgttg tattcagacg
     1261 tccacgagac ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtcgctg
     1321 ttcttctggc ggtaacgcgc tttcttcgca gcaaatttca ctacaacaac ctttgtattt
     1381 ttctgcacat actggacact gaataaacag caggtggcag ccatcatttt tacagttggt
     1441 atggctgtcg cacggcgcac cgcactggtg gcaatgcgcg ataatctcat cagatatacg
     1501 ttcgcccatc cgctcgtcaa aaacaaaatt tttgccaata aaacgtaccg gtaaaccctg
     1561 ctcgcgcgcc ttgcgggcgt attcaataat tccgccctcg atatgccaga ctttattgaa
     1621 tccgttatgt ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat
     1681 aacgattttt ttatctttat gtgcctgcat catctctact gcttttggca gctgctcacg
     1741 gaaggtatct gccggaattt ccagcgcgtt ttcaaagtgc cccacttcat actcatagtg
     1801 gttacgcatg tcgataaaca atgcatcggg atcgtcaagc atggcgttca cttccgccgc
     1861 ttgcagatac tcacccacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac
     1921 gatacgatcg cggaccttca tgcgcagtac ccagaaggat ttcccgtcat catccaacgc
     1981 gatattcagg cgtaagccct ctaaagccgg gtcgaaggca taaagctgcg cgcgaaatgt
     2041 ttcaacattg ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac
     2101 tcgcccaaaa acatccagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg
     2161 atcggcgatg tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa
     2221 cattttggct tttaacgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg
     2281 ttcctgcgag atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac
     2341 atccacacat tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc
     2401 aatttcgccc agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat
     2461 acaattgccc gtataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca
     2521 ccgcactgct tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag
     2581 tcgtgcaatt gccctacccg gttatctacc gcctcggttg tggattagga aaacttgcgt
     2641 tacgttttat gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg
     2701 aaatgagcga acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg
     2761 gcctgatgga aaccggcatg gcgtggttct ggccggaccg cagaatcgcc cgctggacgg
     2821 aagtgatcgg catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag
     2881 ttggcatcca ttttctgaca ctggagcttg gtgcgcggca gtttggtatg caggaaccgg
     2941 ggattggcgt ttaccgcccg aacgataatc cactgattga ctggctacaa acctggggcc
     3001 gtttgcgctc aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaaaccc
     3061 tgaaaaaagg cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg
     3121 tttttgtccc gttgtttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg
     3181 cacggatgtc cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag
     3241 ggtatcagtt gattatgctg ccgccagagt gttctccgcc gctggatgat gccgaaacta
     3301 ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata
     3361 tgtggttaca ccgtcgcttt aaaacacgtc cggaaggcgt tccttcacgc tattaaatct
     3421 cccaagccgg atgcttgaga atagcatccg gcattaccac agcaaatccc cctgatttag
     3481 cgataaaagc tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc
     3541 ttttcttctt atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg
     3601 aaaatgacac ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc
     3661 ttacgggtgc cgccttcagc ctggtaatgc ccttcttacc cctctacgtt gagcagcttg
     3721 gcgttacggg ccactccgcc ttgaatatgt ggtccggtat tgtcttcagc attacatttt
     3781 tattttcggc catcgcctca ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac
     3841 tcatgctatt acgctctgcc ctcggcatgg gcatcgtgat ggtgttgatg ggactggcac
     3901 aaaatatctg gcagtttttg atcctgcggg cgcttcttgg gttacttggc ggatttgtcc
     3961 ccaacgctaa tgctcttatc gccacacaag taccgcgtaa taaaagcggc tgggcgctgg
     4021 gtacgctctc cacaggcggc gttagtggtg cgttgctcgg cccaatggct ggcggcctgc
     4081 tcgccgatag ctacggctta cgtccggtat tctttattac cgccagtgtg ctcatactct
     4141 gctttttcgt caccctgttc tgcatcagag aaaaattcca gccggtcagc aaaaaagaga
     4201 tgctgcacat gcgggaagtg gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt
     4261 ttgtcaccac gttaatcatc caggtggcga caggctcaat tgcccccatt ctgacgctgt
     4321 atgtccgcga actggcgggt aacgtcagta acgtcgcctt tatcagtggc atgatcgcct
     4381 cggtgccagg cgtggcggct ctgctaagcg caccacgact cggcaaactt ggcgatcgaa
     4441 tcggacccga aaagatcctg attacagcgc tgatcttttc tgtactgctg ttgatcccaa
     4501 tgtcttacgt tcagacgcca ttgcaacttg ggattttacg ttttttgctc ggtgccgccg
     4561 atggtgcact actccccgcc gtacagacac tgttggttta caactcgagc aaccagatcg
     4621 ccgggcgtat cttcagctat aaccaatcgt ttcgtgatat tggcaacgtt accggaccat
     4681 tgatgggagc agcgatttca gcgaactacg gtttcagagc ggtatttctc gtcaccgctg
     4741 gcgtcgtgtt attcaacgca gtctattcat ggaacagtct acgtcgtcgt cgaatacccc
     4801 aggtatcgaa ctgatttttc gcctttcata cttgcaaaag cggagaatca gctatccttt
     4861 tccctgaaac ctcatcaact caaagggaga atcgtgatga ccatgtacgc aacgcttgaa
     4921 gaagccattg acgctgcacg cgaagaattt cttgcagaca accccggcat cgacgccgaa
     4981 gatgcgaatg tgcaacagtt caatgcccaa aaatacgttt tgcaggacgg cgacatcatg
     5041 tggcaagttg agttttttgc cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc
     5101 ggtgaagccg cgcaaagtgt ttttgatggc gactatgatg agatagagat acgccaggag
     5161 tggcaggaag agaatacatt acatgaatgg gacgaggggg aatttcagct tgagccaccg
     5221 ctggataccg aggaaggacg cgcagcagct gatgagtggg atgaacgtta atcactcata
     5281 cgggccatga tgaatgtcga tcggcagcag taaagtatcg aaaaccaggg agaatggcag
     5341 atcaaggatc gtgacgtaac gccaggcgga gtcacgcacg tcccattgca caccgggata
     5401 atattggttg ccatgcccct gccccggtat agtgcgacta atgatgctgc cacagccact
     5461 cagcaaggtc accattatgc tcatcacaat taaacgcatt tgatacctct tccgtaaaaa
     5521 ataccggcat aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac
     5581 gctttacccg acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg
     5641 ataagcgtag cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa
     5701 tgccagtgga ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg
     5761 ggaattccag acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag
     5821 gcgacgatcg cgattcagct tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc
     5881 acgggcgatt tccagcacct tgctggcgcg gtgacgcgcg gtcgccattg cggttgccag
     5941 agcgttaaat gacgggttaa acaccgcgtg cataaaacca tcatcaaggg agcgttgacg
     6001 attcatctca aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg
     6061 gatcaggaac agtttccagc gtttggttcg cagaccaacg gtggcacggc tggaaatcac
     6121 cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt gccagccaga acagaaaacg
     6181 cagatccaac cacgccatcc caacagccca cactaaaccc aacagcagct gtgagccgtg
     6241 gcgtttgaat gcttcgcccc aggaagtgga gtcatcatca cgctgcggtg aattccacac
     6301 cacttcccag ccaaggaacg cgctgacgac gaagactgta tggaacaaca tgcgtaccgg
     6361 agccagcaac acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc
     6421 gccgtattct ttcgttcctt tgcaccagat aagcaaaatg ctcaatagct tcggcaggaa
     6481 caacagcacc atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca
     6541 taccgggaac aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac
     6601 ctgcaatgca gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat
     6661 cacgcccgtc aggaacaccg cacggtgaac cgggtgcata cccttcacca ggaacagacg
     6721 gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt
     6781 aggcggcaat tcttcataag aacccgggag atcgtaagca atccacaccc cccaacctgc
     6841 acggcgcatc aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaagaacc
     6901 ttcgcccggc agcggagcca gtgcacaatg ctcgataaac ggtttcacgc ggataatcgc
     6961 gttatgcccc cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag
     7021 tggcccatac acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga
     7081 cgctttcggc gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag
     7141 gccgcataaa caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta
     7201 ctggctgccc cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcgacg
     7261 gcggcggcgg cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca
     7321 ggctttttgc tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc
     7381 aaagtgtttg gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa
     7441 aacacggttc acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt
     7501 taatggttca tcgccaactg ttgacgcgga tatactgtat ttatcgcgac caataagcag
     7561 ttgcaggaag cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc
     7621 aaagaggatc aggataccgg tttgcagcat ataaggcaga agctgcataa aagaaaccca
     7681 cacatcctga ccaaccatat ccataggatt aatcagcgcc cacccctgat aaggaagaat
     7741 ggtcttcata taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta
     7801 acggcggatg gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa
     7861 acgagccaga tagcgcggcg tcacatcgcg tccacgcagg cgatcccaga aacggcctac
     7921 cgggttggta cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt
     7981 aagctgatcg cgcccttcgt catctttaat taactgtcca tcagcaagtg aatctggcca
     8041 ggcttgttcc agacgcgcct ttaccgagcc ttgcggggag tcatcctccc gcgcccaggt
     8101 gcggtgttcg gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc
     8161 tttctcgctt gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag
     8221 gtaactggta gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca
     8281 tttcagtggt tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg
     8341 taaccgggtt atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt
     8401 gcgctgtgac cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca
     8461 caaaggcgat agtaccgtca ggttggcgaa tcaggttcga ctgcttcaca tcccccgttg
     8521 aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc
     8581 tgaaggtgat ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg
     8641 tccagtaagc gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa
     8701 cgctgccttt gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac
     8761 gatcatcgag atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt
     8821 gcgggttttc catcgagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc
     8881 agatccattc gccattacca gcatggatag agagaccgtt agagtcgtgc aactccggac
     8941 gatagttatt tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg
     9001 caacccccag tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg
     9061 tgtcacgccc tggcataact acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca
     9121 atgcataaat ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga
     9181 agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgtgcagaaa
     9241 ggccataaac ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga
     9301 tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa
     9361 ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt
     9421 atttgattcg tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt
     9481 acataccctg atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt
     9541 tgtcatgatt aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg
     9601 gcaagttgct tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga
     9661 catcatcaat actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac
     9721 tcaaccaacg cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc
     9781 tttgtgtgct tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga
     9841 atcccggtat cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca
     9901 ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt
     9961 tatttatcaa ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc
    10021 ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc
    10081 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    10141 tccagtaccc gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt
    10201 agggatccct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    10261 cgaaccgtca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    10321 atttttcgtt atatccgggt acttttccta catgcttttt ttacgctatc ccttgaaaaa
    10381 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact
    10441 gacattaccg caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg
    10501 gctatcattg tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt
    10561 tttactggtg ttagtagtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa
    10621 taatttagaa aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat
    10681 ttttttgtgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    10741 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttgcc
    10801 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    10861 gccgtctcgt ggctgtaccc ttgcagcagc attggcattt gtcgcttatt tactcaacca
    10921 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    10981 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    11041 agcgcgggtg acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt
    11101 aacgctgttt ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct
    11161 ctgtggcctg atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat
    11221 cccgttactg aagtttttgt tctctggtaa accggttgtc aagcgtgaga acgataaagc
    11281 accagcccgt taagccacgc ttataataac cattccacgg gcaatatcga cgccagtctg
    11341 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt
    11401 gcatgacgat caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca
    11461 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    11521 acgaagccca tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata
    11581 aacaccgttt taccatcgat gctaaaggtt ttagcatgca agctggcacc ggaattaccg
    11641 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    11701 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    11761 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    11821 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    11881 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    11941 gccttgcctt cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc
    12001 ggcaacgttc catcaaccag atgatttata aacgggctgg attccatttt gcgtaaataa
    12061 cgatgcgtca tggcatcgtt gtgccaggat gctggtaact cgatgcgatc cgccatttca
    12121 ccttccggga catccagcac ctgctgtagg ggtgaaaccg atttgcaata ccagtagcgg
    12181 gcgaaatcat cggcaacgtc ctctacaact ggtcctattg ccatgacatc taaatccgaa
    12241 aaaagtggct cttcccctgc cccaaaatag gcatcaccaa tatttcgccc ccctaccagc
    12301 gtcaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag
    12361 tcggtgatat aaccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact
    12421 tcaatgcggg gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg
    12481 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaag
    12541 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct
    12601 gccatttcgg ccagacgata ccgggcggca aacgcatcca gacttttctc cagaggaaaa
    12661 atgccacact gaccgggatg ttgcgaacac agtggcagca ccgcactcgc cagccggggc
    12721 aaatcattca tctccttgtt gagtaaggag tctttcgtag aggtgggcgt tttcttcatc
    12781 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    12841 ttttactgcg atttcagccg ctgccgcacg agggtaacca taaactccag tactgattgc
    12901 aggaaaagcc actgacgtat agctgtttgc tgccaccagt cgcaggctat tgagataggc
    12961 atcctgcaaa agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg
    13021 cactacggct ttcgcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca
    13081 atcgccctgc tgctgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg
    13141 atgaatggcc ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat
    13201 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    13261 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    13321 ttctgctcag caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca
    13381 gctttttctg tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat
    13441 tgccgctcat taacgctttg ctggaatagg ttctgcccgg aacagagcca ttctgtgggc
    13501 agttcatttc tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc
    13561 cataaaaatg gatcacgccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa
    13621 acatactgcc gagcattaga gagtctaaaa aaggtctgaa catttattct atttcctcaa
    13681 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga tccgcagcag
    13741 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    13801 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    13861 tcacccgatc gtccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag
    13921 caggcaatga gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    13981 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    14041 tgtacatatc cccttgctgg gtcgtattaa aggttatctg actggaaagt gccgcaagga
    14101 gtaataacgc attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttcttta
    14161 atacagagga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtg
    14221 acgttgacgc tggagttaga tgcagtctgg tcaacagcag caccgttacc accaccgaac
    14281 tgtttaacag tcatagtaga atctttacca ttccactgat caagagtagc gctgttgccg
    14341 aaaccacgct gagtcagatc gattgagctg tcatcagaac cttggccaac atctgcgccg
    14401 ttaccgccgc cgtgctgggt aatggtcaga tcagagttac gggcgtcagc ttgcagagca
    14461 agagcagagt taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta
    14521 ttaccgccac caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg
    14581 ctaccagaga atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc
    14641 catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg
    14701 cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt
    14761 attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact
    14821 ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc
    14881 aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc
    14941 ctgccgtaac tgagcactat tattagtccc agcttgacca attatggctg cctgattaaa
    15001 tgaagactta ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc
    15061 tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt
    15121 tttcatgttg tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg
    15181 ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa
    15241 atatttttaa taactcaccc gcgcgttgta tttttctttt tttgttcata ccaaagtatt
    15301 aaaactcacg taaatacgct gttatctacg caaaaaatat tttgttttct tttaaatctc
    15361 cgttttccgc tatcaaaaat caccagacaa tcattcttct tgcccgtcgc tgattgctgc
    15421 gatatgtctt gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac
    15481 ggaggtgtgg agaaaaaaca agaacgtttt acatgacgaa aggactacac cgaaatattt
    15541 tttatatgca ttattagtaa gttagcacca tttgtatgat tttttaaaat tgtgcaataa
    15601 aaaccaaatg tacaactttt ctatcatttc taaacttaat aaaaccttaa gattaacatt
    15661 ttaatataac gagttacatt tagttacatg tttaacactt gatttaagat ttgtaatggc
    15721 tagattgaaa tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat
    15781 tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc
    15841 tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt
    15901 attcatggtc atacattatt gttgatcact aaaccttctt tgcaggcgac agctctcttg
    15961 cagcacctta agcaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg
    16021 gacgatatct cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
    16081 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
    16141 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
    16201 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
    16261 gaatgctact ttacgcaaaa gcttgccagt tacctgatta cgcattcagg taactatcgt
    16321 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataagctg
    16381 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
    16441 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
    16501 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
    16561 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
    16621 attgttaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
    16681 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
    16741 gggaagctgg atcacaataa cggtgaatca ggacgttatt ttccagactt ttttatttcc
    16801 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
    16861 aaatcgtcgc cagataaacc aagcgttatt aagtacgggc gatttggcgc atgatgaatt
    16921 ttaaataata aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
    16981 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
    17041 tggtggtaac ccaaataatg gcgctttttt gttaaatagc gctcaggccc aaaactctta
    17101 taaagatccg agctataacg atgactttgg tattgaaacg ccctcggcgt tagataactt
    17161 tactcaggcc atccagtcac aaattttggg tgggctactg tcgaatatta ataccggtaa
    17221 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
    17281 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
    17341 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
    17401 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
    17461 gccgccagac cgacattaat gcctcgtgct cagagctaca aagatttgac ccatctgcca
    17521 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
    17581 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
    17641 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgccaagg cttacaaaac
    17701 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacggt tgccattaat
    17761 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcaattatc
    17821 ggttatgaaa gcaacgttaa atcgggcggg gttggggcaa gatattttgg gatcggtgca
    17881 gacacgcaat accagcttga tcagattgcc gtgaacctgc gcgtcgtcaa tgtgagtacc
    17941 ggcgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcg
    18001 ggggtcttcc gctttattga ctaccagcgc ttgctggaag gggaagtagg ttacacctcg
    18061 aacgaacccg ttatgctgtg cctgatgtcg gctatcgaaa caggcgtcat tttcctgatt
    18121 aatgatggta tcgaccgcgg tctttgggat ttgcaaaata aagcagaacg gcagaatgac
    18181 attctagtga aataccgcca tatgtcggtt ccaccggaat cctgatagat aaaaatgccg
    18241 gaattatcat ccgacatttt acggtttaag cgaaagcgtg agtgcccctc acgctttatt
    18301 tttgatttgc tgctcacggt gagccgtctt tgcactacgc gccgccaacc acaggccgct
    18361 gtttttcatt gcgtaaccaa ataccagtcc aagcgccagc gaaggtaaca ccagtttcca
    18421 gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag
    18481 cagcagctgt ttggcctgga tacacatcag aaacgcgaca ataccggtta tggcataacc
    18541 gagtatttcc agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac
    18601 gccactaagc agggttgcag cggagatcgc cagccctttc agcccacctt gtgggcaggc
    18661 aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac
    18721 ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat
    18781 gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac
    18841 aacaaattac ccgttgcagt tatgagtgat gcagatcaca tttactcttc agaatcttct
    18901 tccagctcgt cccacattgc actaatagca tcgcgggtta gcggtgccat ggtgcgccag
    18961 aaaggcgtag ttgcatgggc ctccactttg ccaaggaacg caccacacca gggtaacaga
    19021 tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc
    19081 caggaagccg caagcagcaa tgtgccgatg tgatcggctg gcgtatccgc tagtggcatc
    19141 cctcgttcgg aaagaaaagc gcgcacttcc gcttccgttg cgccctcaac ccatgcgcta
    19201 cgatatggcg gcacagcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact
    19261 tgcgtcatat cacagctttt ctgtaaacgc gtcagtaact catcctgctc cagtggccag
    19321 ttcgcagcca gtttcccctc acgaatcagg gtaaacaacg gcaccagtaa aggatcttgc
    19381 ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc attcattaat
    19441 atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag
    19501 attcaaggaa gttaagtaag cggcgcggag aaacattcaa aatgcgctct ggcggaaaat
    19561 ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac gcagtgtgag
    19621 aatccgagcc taatgccacc caacctccag cgtcacgtac cgctgcggct accgcccgac
    19681 agttgtcttc actgccctta cgtgagtgta aaaatgagga attattgatt tccagcgcca
    19741 cctgatgttt cgcggctgct tcagcaacgg ctttaacatc tatttcatat ttcggatttc
    19801 cgggatggct tattatatgc acattgccgc tggcgatagt ggcgatcatc gcttgcgtat
    19861 tggtcgcttt gtcatgcggc gcaaacaccg gctcatgaaa accggcaata atcagatcca
    19921 gcgaatcaaa cattttgcca ctgcagtcaa tttcaccgtc aacattttta atgttagctt
    19981 cgatgccgcg caggatccca accccatcaa ccactcgcgg ccagatacgc atgttaatga
    20041 agtgccagtg atgcggcgca tcttccatat ccgggccatg atcggtgatc gcaaaaagtt
    20101 taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgcgtgc
    20161 tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc
    20221 cgccaaggat agcaggaaac ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc
    20281 cgcagaccct ctcccctttt tcgagctggg caatggtgcg cgaaatgtaa tccacagctt
    20341 cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga
    20401 gcggactttc cggcggtaag ggttcacgat taaaaacatc cagcattgcg cctttaactt
    20461 tgccgctatc cagcgccgcg agcaggtcat cttccacgac atgaacacca cgcgccaggt
    20521 tgaggagata cgcgccatcc ggtaattttt cgagtaattg ttgattaata atgccgacgg
    20581 tttcaggtgt attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaga
    20641 gttcttcccg tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc
    20701 aacgcagcgg gaagcgccag gtttgcagac tctgtgcaac tttactgccc agtacacctg
    20761 cacccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg
    20821 aactattttg ctggatgcgg taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga
    20881 cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag
    20941 aaggcttcag catttcaggg tgagcctgta gcttgctcaa aatagaatca acacctgccc
    21001 caagtgcaaa caccgcttta agatcgcgcc cagccagcat ttcaacagga ggatgccaga
    21061 ctaaagcata atcggctgat tcattgtctc cgcttttcca tgctctgact cttgcctgag
    21121 gaatagcttt gtgcagtgcc tcaatccacc attgggtatc gaacgttggg tgataaaaga
    21181 tgatatccat actgactccc gaagagtgtt ggcgaaattt attcgcactt atcgttatga
    21241 tctacaaaga ccagcagcat aacaaatccg tggtcggtgg caaaaaaagc aaatttcgct
    21301 tattaaaact gcatattgat tgaagtttga ataatcgagc gattttttaa aaaagtttgt
    21361 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
    21421 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
    21481 tcgggggttc gaatcccccc ctcaccgcca gattacaagc gcatgttatt gtttaacatg
    21541 cgctttttta tttatccttc cagacagtat gccaccgggt atgccatctg aaaccggttt
    21601 atatgcccca cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
    21661 tatactgcca gcatgtcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
    21721 tctggcttcc tgaacgtgat gtgcgcagag cccttcttaa agtatttaat aatgaacatc
    21781 tcatcttcat agcattcttt tccctgaaga gaccggatgt gatcgtccag atgaatagtt
    21841 atgttttccc tgttgtcggg gatcggtttg ccgctgaaca gatgcagcat tctttccagg
    21901 tcggcaagtc tatctgcctg ttgcccggtg ataagatgca atccccactg gtcccacctc
    21961 accagattgt tgacgataat tttattgcca aatttacagg ggcaattggt tttgtaattc
    22021 cagctcaacc ctctgaagac gttgatcact cctcgctcaa acacttcatc cttattctgg
    22081 tgtagctgct caaacgtact cagaatattc tcttcactga tttccggaaa attatcgtac
    22141 tccagtgacc ggtaccatgt atcacgggcc tgtgcatcca ttaaggaaag catcccggag
    22201 cattgcatca ggtcacgcca gatttctcga tcgagattgc aggtgatggc tttcattgcc
    22261 gtttcaggtt tttccatcag ccagcaaccg cagcgaaaat cctgtttcat tgcccagtcc
    22321 agtgcggttt taccgccaat actttctgtc attgctgata tttcagccag ttgccggata
    22381 agtgtgtcga tttgcagcag tgtactcttt cgtacagcca gaatgtggcg gatactggtt
    22441 gagcagataa cgtccgtatg cccggtgacc agttccggac gggagattgt gatgtctgac
    22501 attatcggtt tccctttggg tttataaacg aaaacgccag ccctgttcag gctgacgtca
    22561 gggtaatgaa gtctggtgag taagactcac cggttctgtt gcagaaactg cagttggcgc
    22621 agggtacgga tatcttccct ggcctgttca atgcagggca gcagtgctgc cgaatccgtt
    22681 ttatcctcat cgttgtcaaa gataattccc gattcgcagt cgatattgtc ctgcagccat
    22741 gcaatcagaa tatccagcgc tgtttccgtg gttaatgatt tcatgttgtt tctggttacc
    22801 agtcgaaagt ggggaaaccc ggcagacatc cggcactggc atccagatga atgagactga
    22861 cgccataacg tcggatgagt gtgacaacca gacggcggaa cgtttttgac agccccaggc
    22921 gtttaagccg cagaaccggg tatgaccacg catcggtacg taacagatat ccgctacctg
    22981 tgaaatgaat ccattctgat tcaccaacgt cactggtctg atgcgacagc gagtacagcc
    23041 aggcgttatc cttttccgtg atatgtgcgg tactgcagcg tatgccggca agggttgcaa
    23101 acggtggtag tgtgcaggtt gactgttggt cggattcctc caccacgcgg agtgaataac
    23161 cgttttcagc gaccttgtta atcagttcag tgagattaac accatcgacg tcaacgacaa
    23221 tttgccccat gttcagtgcc tgcacattaa ttctgtcggc ttccagcatc agggaaagtt
    23281 tcatcgtttc tccggatgct tacccagggt aatgttattt accgttctgt aattgtcgcg
    23341 ggtcatcaga cctgttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga
    23401 cgaagttcct gcgctgaatc ccggctggtc ggtacgcacc agcgcgtatt tttccacgag
    23461 aaaattcacc gcatcacaca gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc
    23521 atcagcgaac ggtgtgtcat tcagcgtaag gccatagtgc tggtccagca gtcgggtgag
    23581 cagtgtctgc cagatttcaa caggagatgg gcgtgaactg gccgactgcc cgggtaatac
    23641 aggtaatgtt ttcatactga agattttcct gatatgcaaa gatgaaaaat gggaaagtgg
    23701 tgtggtgaaa acaccaggcc ggagcagagg gttattctgg agagttactt tttcgtttcg
    23761 ggcgtcggat aaacagccat ataaacgtag ccacaactgc cgagggtgtc ggcttcgcag
    23821 gtcagccctc tggcatacag cgtgacggta tgctgatggc ggggattcag ttcaccgctg
    23881 gtgagcatga gttccagttg tttcatcagc agcggaaagg cctggtccag gtggtacgca
    23941 tctgcatcgc tgaacaggcc tctgatacca gcgcggtcgg caaggtaatg caaccggtta
    24001 ccctcctgca ccagacgggc gccgaaacag ggcgtcacgg tgcagggcag cccccaccag
    24061 gggcggtcgt gattgccgtc gggaagtgtg tctgacacga taaaatcctc acagaaaatc
    24121 ggttaagaat ggtccggtgg tggcgataat tctgctcatc agaatgccct gactcagttc
    24181 agggtgacgc tcatcagccg gacatacggg ccaaaactgt ccttacggcg ttcagcaaac
    24241 acggccagca caccgggaat atcctgtact tcacgaccgg tatacgcccc ggcactgccg
    24301 tgccagcggt acttaccggt gcagaacgga aaaagacggg aggccggatg ctgttggtga
    24361 atacgcatgg cttcaccacg ggtgatgatt ttcatgatgt gatacctctg aagacagaag
    24421 ataaaagtga aaacaggtat gatgtggttg tgacggtgac gggttaaagc agaccgtgtt
    24481 cggcaaagga gaaaacctgg ctgccaccga ctatcagatg gtccggcacc cggatatcca
    24541 ccaggcccag tgcctgtacc agacgttccg taataagccg gtctgcctta ctgggtgtga
    24601 cttcaccgga cgggtgattg tgtgccagta ccacggcagc ggcattgtgg tacagggcgc
    24661 gtttaatcac ttcccgggga tggacttccg tgcggttgat ggtgccggtg aagagggttt
    24721 caccggcaat caactgattc tggttgttca gatacagcac ccggaattct tcacgctcca
    24781 gtcccgccat gttcagaatc agccattccc gtgccgctcg ggtggaggtg aaggccacgc
    24841 cgggttcatg aagatggcgg tccagggttt tcagggcccg cagaatgaga ctgcgctcgc
    24901 cgggcgtcat ctctccgggc agaaaggaaa gttgttgcat tgtgcttctc tccattcagt
    24961 cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc cgcaaccggt
    25021 aataatggac cgtcatggca taacactccg tacgacaggc atgatgactg tacgtcatca
    25081 gacaggcagc aatgccggcg gcttccgggc tcatttcagc gcggttaccg ttcatggcat
    25141 tgaacagtac ccagttttcg ccatcatcgt catccggttc gggggccata aatgcgccac
    25201 cgttgttcag tgtgtacaga ttccagatac caccgcagta gtcttcgcaa agacggtcca
    25261 tccagccgaa gatacggggc tccagggtca cccactgtgg aatgaggcca aaatgctgcg
    25321 gccagaagct gatgcgctgt tcatcaggga ctatggtggc aaccagctga ggctggtcac
    25381 tccctgatgc agttgttacg gaaacagaag gtgtggtgga gctatgcgaa aaggttgtca
    25441 tgaaatgatt ccttataaaa agcaaatgaa tgaagaagac tcgggggcag ggacagacgt
    25501 gagtcaggac ggcgctttca ggtaaacggt atcagcgcat gctctccagc agcgtttccg
    25561 ccatcaccca caatgcgcga ttgagcttga tgtcggtgtc gatactgtga atggcacggg
    25621 tatggatacg ttttcctttt gcactacggc cggaaatccc gcctttcagc atattctcct
    25681 ggatggtctg atatgcgctc cacaggtcct taccgtaatc ctcccggcgt cgtggtgtca
    25741 gaatgtcggc agtggtgacg ggttgatgtt catcaccata acggtaagtc agcgccgcct
    25801 gtgccagtgc ctgacgtgcc ggtggcggca gaaccagaga ctgcatggca tcacgctttt
    25861 cctcaatccg gtcaaaaacg cccactacct cgtaagcccc ttcaatgact ctgtccacca
    25921 catttccccg gtggggcaca cggacttccc ccagagactg accgcagacg cagccgttct
    25981 ggcagacgaa tctgaagtaa cccggcagca tctggtagct ggaggtaccg tcatgggagt
    26041 tgagcagaat aatttcaggc acatgctgac cggttatctg tccggcccgc cgcagacgca
    26101 gcatgtgttt ggtatattcc cggcggcccg ggtcacgtac gcgggtctgg caggcgaaga
    26161 atggctgaaa gccttcccgc tgcaggcttt ccagcacggt gatggtggga atccaggtat
    26221 agttctcgct tctggatgtg tgtttctctt ccccgaatac gctggggacg acctgcatca
    26281 gttcttcacg agttaagggg cggtcacggc gtatctggtt cgcataacca aaacgactgg
    26341 ctaatcgcat gatttattcc ttatgaatga gttagcggta taaat
//
LOCUS       DAZGTJ010000074        26280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000074 DAZGTJ010000000
VERSION     DAZGTJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.074"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..727
                     /locus_tag="ACHK8E_003611"
     CDS             <1..727
                     /locus_tag="ACHK8E_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP0345826.1"
                     /translation="FADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSL
                     KPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLT
                     PYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQ
                     QTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQY
                     KHLFAHGEYIKGITSLIKYKLKK"
     gene            737..1429
                     /gene="rfaY"
                     /locus_tag="ACHK8E_003612"
     CDS             737..1429
                     /gene="rfaY"
                     /locus_tag="ACHK8E_003612"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFP0345827.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            1455..2483
                     /locus_tag="ACHK8E_003613"
     CDS             1455..2483
                     /locus_tag="ACHK8E_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFP0345828.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFGQ"
     gene            2565..3548
                     /locus_tag="ACHK8E_003614"
     CDS             2565..3548
                     /locus_tag="ACHK8E_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0345829.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTDFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEDNNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSKNRTYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELKDCNLLGKRDKRRAKILASKNYILWRMVRLVSRKKNKR"
     gene            3594..4847
                     /locus_tag="ACHK8E_003615"
     CDS             3594..4847
                     /locus_tag="ACHK8E_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP0345830.1"
                     /translation="MSFCWNEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLTRMNDLNRDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVALTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(4917..5897)
                     /gene="rfaC"
                     /locus_tag="ACHK8E_003616"
     CDS             complement(4917..5897)
                     /gene="rfaC"
                     /locus_tag="ACHK8E_003616"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP0345831.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
     gene            complement(5901..6947)
                     /gene="rfaF"
                     /locus_tag="ACHK8E_003617"
     CDS             complement(5901..6947)
                     /gene="rfaF"
                     /locus_tag="ACHK8E_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP0345832.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(6957..7889)
                     /gene="rfaD"
                     /locus_tag="ACHK8E_003618"
     CDS             complement(6957..7889)
                     /gene="rfaD"
                     /locus_tag="ACHK8E_003618"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP0345833.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            8103..9299
                     /gene="kbl"
                     /locus_tag="ACHK8E_003619"
     CDS             8103..9299
                     /gene="kbl"
                     /locus_tag="ACHK8E_003619"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP0345834.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            9309..10334
                     /gene="tdh"
                     /locus_tag="ACHK8E_003620"
     CDS             9309..10334
                     /gene="tdh"
                     /locus_tag="ACHK8E_003620"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP0345835.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            10573..11607
                     /gene="waaH"
                     /locus_tag="ACHK8E_003621"
     CDS             10573..11607
                     /gene="waaH"
                     /locus_tag="ACHK8E_003621"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0345836.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(11594..12553)
                     /locus_tag="ACHK8E_003622"
     CDS             complement(11594..12553)
                     /locus_tag="ACHK8E_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP0345837.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(12557..13816)
                     /gene="envC"
                     /locus_tag="ACHK8E_003623"
     CDS             complement(12557..13816)
                     /gene="envC"
                     /locus_tag="ACHK8E_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP0345838.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(13850..15394)
                     /gene="gpmM"
                     /locus_tag="ACHK8E_003624"
                     /gene_synonym="pgmI"
     CDS             complement(13850..15394)
                     /gene="gpmM"
                     /locus_tag="ACHK8E_003624"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP0345839.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15639..16070
                     /locus_tag="ACHK8E_003625"
     CDS             15639..16070
                     /locus_tag="ACHK8E_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0345840.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            16212..16463
                     /gene="grxC"
                     /locus_tag="ACHK8E_003626"
     CDS             16212..16463
                     /gene="grxC"
                     /locus_tag="ACHK8E_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP0345841.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            16526..16993
                     /gene="secB"
                     /locus_tag="ACHK8E_003627"
     CDS             16526..16993
                     /gene="secB"
                     /locus_tag="ACHK8E_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP0345842.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            16993..18012
                     /gene="gpsA"
                     /locus_tag="ACHK8E_003628"
     CDS             16993..18012
                     /gene="gpsA"
                     /locus_tag="ACHK8E_003628"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP0345843.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            18092..18913
                     /gene="cysE"
                     /locus_tag="ACHK8E_003629"
     CDS             18092..18913
                     /gene="cysE"
                     /locus_tag="ACHK8E_003629"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP0345844.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(18966..19439)
                     /gene="trmL"
                     /locus_tag="ACHK8E_003630"
     CDS             complement(18966..19439)
                     /gene="trmL"
                     /locus_tag="ACHK8E_003630"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP0345845.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(19512..20702)
                     /gene="lldD"
                     /locus_tag="ACHK8E_003631"
     CDS             complement(19512..20702)
                     /gene="lldD"
                     /locus_tag="ACHK8E_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP0345846.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            complement(20699..21475)
                     /gene="lldR"
                     /locus_tag="ACHK8E_003632"
     CDS             complement(20699..21475)
                     /gene="lldR"
                     /locus_tag="ACHK8E_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP0345847.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            complement(21475..23130)
                     /gene="lldP"
                     /locus_tag="ACHK8E_003633"
     CDS             complement(21475..23130)
                     /gene="lldP"
                     /locus_tag="ACHK8E_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0345848.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            complement(23542..>26280)
                     /locus_tag="ACHK8E_003634"
     CDS             complement(23542..>26280)
                     /locus_tag="ACHK8E_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFP0345849.1"
                     /translation="DSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGS
                     QLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDA
                     LLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDTSKYIADT
                     LGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATANDGNGAFSA
                     AHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAP
                     TYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNVADGNIGE
                     GSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFND
                     ASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVKGSRDTSV
                     SEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVAT
                     TPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSN
                     ARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGS
                     ADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVSTGSTKYF
                     KTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITN
                     VAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRISNVSAGV
                     NNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYT
                     PGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
BASE COUNT         6506 a         6416 c         6571 g         6787 t
ORIGIN      
        1 ctttgctgat ctgccgacgt cacagttttg gtcgtacgca acatacttca gggtattgtc
       61 ttttgaatat ctgagtgaaa gtatttctac actgctgtat ctggatgccg atgttgtttg
      121 taaaggaagc ctgaaacctc tcacagaaat tatatttaaa gatgagtttg ctgcggtcat
      181 tcctgacaat gatagtactc aggcggcatg tgcaaaacgc ctcaacattc ccgaaatgaa
      241 tggacgttat ttcaatgcag gcgttatcta tgtcaatctt aaaaaatggc atgaagcaaa
      301 tttgacaccg tatttactca cgcttttacg aggggaaact aaatatggct ctcttaaata
      361 tttagatcag gatgcgttga atatcgcatt taatatgaat aatatctacc tcgcgaagga
      421 ttttgatact atttataccc tgaaaaacga acttcatgat cgtagtcatc gaaagtatca
      481 gcaaaccatt accgataaaa cagtattgat tcactataca gggataacta aaccatggca
      541 tagctgggct ggatatccat ctgcatcata ctttaatatc gcgcgtgaac aatctccctg
      601 gaagaaatat cctcttaaag aggcgcggac tgttgcagaa atgcagaaac aatataagca
      661 tctgtttgcc catggtgagt atattaaagg tataacttca ttaattaagt acaagcttaa
      721 gaaataaatt actcttatga ttacaagtat acgctatcgc ggcttctcat tttattacaa
      781 agataacgat aataaatata aagaaatctt tgatgaaata ctggcctata attttaaaac
      841 agtaaaagta ttacgtaata ttgatgatac caaagtatcg ttgattgata cgaaatatgg
      901 tcgctatgtt tttaaagtct ttgctcccaa aacgaaaagg aatgaacgtt tttttaaatc
      961 ttttgtaaaa ggtgactact atcagaattt gatcgttgaa accgatcgtg tcagaagtgc
     1021 aggtcttact tttcctaatg atttttattt tctggctgag cgtaaaattt tcaactatgc
     1081 cagtgttttt atcatgctta tcgagtatgt cgagggcgta gaactcaatg atatgccgat
     1141 tattccagaa aatgttaaag cagaaattaa ggcttcaatg gaaaaattgc atgccttaaa
     1201 tatgctatcg ggcgaccctc accggggtaa ctttatcgtt agtaaagatg gtgtcaggat
     1261 aattgaccta tccggtaaaa gctgtacggc agagcgtaaa gctcgtgatc gtctggccat
     1321 ggaacgtcac ctgggtattg ccaacgaaat aaaagattat ggctactatt cagtaatcta
     1381 cagaaccaaa ctacgcaaat ttattaaaaa attaaaaggc aaagcgtaaa ccacacagtc
     1441 aaaacggaac caacatggat ttattagctg agagtattac tgaagtcgct gtctctgggg
     1501 aaattgctaa caccgatcgt gtgttaaata tcgcttacgg tattgaccgt aattttttat
     1561 ttggtgcggc agtatctatg caatcagttg ttatgcataa cccggacctt gcggttaagt
     1621 ttcatctctt taccgactac attgatgaag attatctaca acgtgttaac gcttttacca
     1681 gcaaaaatgc taacgttgaa gtaaaaattt ataaagtctc cagtgccttt attgatatct
     1741 tccccagcct gaaacagtgg tcttatgcaa cattcttccg tttagttgcg ttccagtatc
     1801 tgagtgaagt tattgaaaat ctgttatata tcgatgctga tgtcatctgt aaaggctcat
     1861 tagctggatt gcttgatatt aattttgatg gcgataagtt cgcagctgtt attaaagatg
     1921 tgccttttat gcaggaaaaa ccagcgaagc gtctggctat agagggactt ccaggtaatt
     1981 atttcaacgc cggtgtagta tatctgcagc ttgaagcatg ggcgaaaaat gattttatga
     2041 ataaagccat tgctatgctg gcaagtgacc cgcagcacac gaaatataaa tgccttgatc
     2101 aggatatttt aaatattctg ttctttggtc attgtatttt tattagcggc gattatgatt
     2161 gcttttatgg cattgactat gagttaaaaa ataaaagcga tgaagattat aaaaagacca
     2221 ttaccgatga tactaagctg attcattatg ttggcgtaac gaagccctgg aacgactgga
     2281 cgaattatcc ctgccagaag tattttaatg aggcttatca ggcttcttgc tggaatgatg
     2341 tggcgtttat tccagccacg aatgaaaagc agtatcaagt gaaataccaa catgcaaaga
     2401 aaaatggtga tacgtttaac gcttttattt acttcattaa attcaaatta aataagtata
     2461 aaagaaaact atttgggcaa taataaaaat catggatgtc tgagttacaa taataattaa
     2521 ggatgagtac tatcatattt tattagttca ggcaagagaa cacgatgagc aatgattacc
     2581 ctttagtatc cataataata ccgacgtata attcatctga ttacattact gaaactctaa
     2641 cgaaattaga aaaacaaact tacccaaatt ttgaaattgt tattgttaat gatggttcta
     2701 aagataacac atcaaatgtt ttgagagagt atgggttaac ccactctcga ttaattatta
     2761 tcaataaaga aaatggcggt gtttcgtctg ccaggaatac aggtatccgc aaggcgcaag
     2821 gacagtttat atgttttatg gatgatgatg atgagataga tcctaactat ctgctgaaga
     2881 tgtattccag acaacatgag acgggagggg atgcgattta ttgtgggctt tatggacatc
     2941 atataaaaaa tggtgttact tactcaccta taaatacaga ttttaatgaa gggtctttac
     3001 ttttcgattt tttttataaa aaggtcagat tccatatagg gtgcttgttt ataagaaaac
     3061 aactcctgga agataataat cttttttttg atgaagattt acgactagga gaagatctgg
     3121 attttatcta tcgactgcta attacatgcg atatgtatgc ggttccatat tatatgtata
     3181 agcataacta tagagaaaat tccttaatga actcatgtag aaccatcact cattatcgac
     3241 atgagtcatt tgcgcacgaa agaatctact cttctgtgat gcagttatac aaaggtaacc
     3301 ggaaagaaga aattcataca ttattgagta agaatagaac ttatcataaa actcgttatt
     3361 tatggaatgt tctacttaat ggcgattttg aattattaaa tcaattagtt gaaagcaatg
     3421 aaaaagaatt gaaagattgt aatcttcttg gcaagagaga taagaggcga gcaaaaatat
     3481 tagcatcaaa aaactatatt ctctggagga tggtaagact ggtaagtaga aaaaagaata
     3541 aacgttaggt cttgcattaa ataacccatc ctcgtagcat aggttgaaat tatatgtcgt
     3601 tttgttggaa tgaaattaac tctggtgtca agtctttaat tctcatatta tgtatgttgt
     3661 ctttaatgac tttgtctttg tgggatgatg ttgcaacaaa gtttcttcat gcagctggaa
     3721 ttatatctgc attatatttt ctggcgacac caaaaaaaac aataactaat aatcctactt
     3781 tgttaatttt catctcatta tgtcttttgg gtatcgttaa tatcatctgg tattcacatt
     3841 ataaagtttc aggctcggtt tataccaatg catatcgtgg cccaatggaa actggaaaaa
     3901 ttgccttgtg tagtgctttt attttcttag ttctttttgc taaagatgag atgagaacca
     3961 aaataaaatt tgggaaacta attctgttcg catccctggc aacgcagtta cttttttttg
     4021 cgcatgccat gtggcaacat ttctatttaa acgtcgaccg tgttgcatta tcagcgtccc
     4081 acgctacaac agcaggctac atcatccttt tcccttcttt actggcatca attctcattt
     4141 taaaatccga ctttagacat aaaacaacat tatatacaat taacttcatg cttagcttat
     4201 gtgctgttat agtaactgaa acgcgtgcag ccatattagt gtttccattc tttgcgttac
     4261 tattaatcgt aatggatagt tatattaata agcgaattaa ttataagtta tattgtttta
     4321 ttgcgattgc attattagca ggtgtatttt cttttaaaga tacattactt acgagaatga
     4381 atgacttaaa tagggattta gttaattatt cgcatgataa caccagaact tcagtcggtg
     4441 cccgtctggc aatgtatgaa gttggtttaa aaacatattc tccaatagga caatcactgg
     4501 aaaaacgtgc ggaaaaaata catgaactag aagagaaaga gcctagatta agtggcgctt
     4561 taccctttgt agattctcat ttgcataacg atctcataga tacgttatca acgcgtggta
     4621 ttcctggagt agcattaaca attttagcct tttcagcaat attcatatat gccttaagaa
     4681 ctgctaaaga accttatatt ttaatcttgc ttttttcact actggtagta ggcctaagtg
     4741 atgtaatact cttttctaaa ccggttccga ctgctgtgtt tgtcaccata atattgcttt
     4801 gtgcttattt taaagcacaa tcggaccaat gtttattaga taagtaatga atatgtgaaa
     4861 taaaatcagc aaggaagctg attttatttc aaaattatct attaaagagt tgtaatttaa
     4921 atcatggcag ctttttcagc gttttcttca atgaattgct taacagcatt tgctgtcaat
     4981 tgagacaact cattccccgg agccctacaa accatctgat tcttcccata cccaccaatt
     5041 aatcccggat cggtaggtcc ataaaccgtg atattgggtc tatccagtgc cgccgttaaa
     5101 tggcttaacc ccgtatccac cgacactaca aatttagccc cggccagcac gcgggcaacg
     5161 ccttccagac tcatcttcgg caatacttca acataagcaa atccttccgc cagtcgtttc
     5221 gcccgttctt cctcatgcgg cgcgccccac ggaagtttaa tccgtattcc tgaatcagcc
     5281 agtaaaccaa tcaattctcg ccagtgttct tccggccagt gtttatcatc acgggtcgtc
     5341 gcatgaagaa atacggcata ttcgccagca tctgtaggca ggttcgtcag gaaatgctgt
     5401 gcgatagcat aatcgccctg ggtttgcggt ttgctgtagc ccagactttt ggcaaacagt
     5461 tcgcgggtgc gttctacggc gtgctgctgt tttgcaatat gatgcttacg attgtaaaac
     5521 aggctggcta aaggttcgcg agcggtttgc cagtccatgc catgctttac gccatgcgcc
     5581 agacgcgtca ccagcgccgc gctttttacc agcccctgag cgtcgataac tgcgtcatag
     5641 ttctctgctt gtagcgcttc acgaaacgct ttgcgttcag cttttatggg ggccgagaac
     5701 caggctttac gccagcgacg tattgccaca ggaataactc gctcaacggc ggcgtgccag
     5761 gaaggaatct gtgcgaaccc ttcttccacc acccagtcaa acttaatccc tgggattgcc
     5821 tgctgggcat cagtgagtgc gggcaacgta tggagaacat cgcccatcga cgatgtttta
     5881 acgatcaaaa cccgcatccg tcaggcttcc tcttgtaaca atagcgcgtt gagttcttcc
     5941 agtacgcgct ggggagtaat gtcgatcaag ctctggtgat aaccctccgc agcgtcacct
     6001 ttacgcactt tgtgatagcc ggtaatcagg cggatcacgc gcgctttatg ggatagcggc
     6061 ggtgtgaagt ccgggctact cggaccatac agggcaacca gcggacgatt gagcgccgcc
     6121 gcaacgtgca tcaggccaga atcgttagta acaatggctt tacaggctgc aatcaggata
     6181 accgcttgat caagctgtgt ttcccccgcc aggttacgac accatgcctg ctgctcggta
     6241 ttcaaagcgg caagaatctc attgcccgct tcatgatctt tcgccgagcc aaacagaacc
     6301 acctgataac cttcatcaat cagttgcttt gccagctccg catagtggta gtgtggccag
     6361 cgttttgccg gaccaaactc cgcacccggg caaaaaccaa tcatcggacg ttctgatgaa
     6421 agcgaaaatt gattacaggt atatgatttt tcaccttcgc tcacctgcaa ctgcggccat
     6481 aacaatggct gcggcagatc ttgtgcggta cgcataatac ctttgtcata ggccagcgcc
     6541 acatagcgtt ccaccatcag cggccaggct tctttatcca gcacacgcac atcgttgagt
     6601 aaaccgtagc gcatttcgcc gcgccagccg gtgcgatgag gaatacccgc gaagaaaggc
     6661 actaatgcgg atttgaagga gttgggtaaa acgtaggcgc ggtcgtaacg tttttcgcgc
     6721 aggctatgac ccagtttgcg gcgttcgccg atttccagcg ctccgtgacc gagaggcata
     6781 ggaatagctt cgttaacttc cggcatccgc gataataatg gacggcacca tgccggtgcc
     6841 atcacgtcga ttatcgcctg gggatagcgc gcctggagcg tgcgatagag actttgcgac
     6901 atcatcatgt cgccaaccca agacgggccg atcaccagta ttttcatgca gagctcttat
     6961 gcgtcacgat tcagccaggc catgtattcc gttacgcctt cggcaacggt tttaaacggt
     7021 ttgtcgtaac ccgccgcgcg cagatttgtc agatctgcct gcgtgaacgc ctggtagcgg
     7081 cctttcagtt tatccgggaa cggaatgtat tcgatttggc ctttcttgtg ataagcaagc
     7141 gtagcatctg ctactgcctg gaaggattcc gcacgaccag taccgaggtt gaagatgccg
     7201 gaaacgccat tttccaggaa ccacaggttt acatctgcca catcgcccac atagacgaag
     7261 tcacgtttga agttctcgct accttcgaac agcttcggtg attcaccgtt attaagctgg
     7321 gtgttgagat ggaaagcgac gctcgccatg ctgcctttat ggccttcacg cggtccataa
     7381 acgttgaaat aacggaagcc aacaatctgt gagttcgctt caggcaggat ttggcgaaca
     7441 tattcatcaa acaggaattt tgagtaaccg tagacattca acggtttttc atattcgcgg
     7501 gattcaataa agtcggaggt gcgtccaccg taagtggctg cggaagaggc atacaggaac
     7561 gggatttcgc gctccagaca gtagtgcagc agctctttgg agtattgata gttgttatcc
     7621 atcatatact tgccgtccca ctcggtggtg gaagagcacg cgccttcgtg gaaaatcgct
     7681 tcgacatcgc cgaactcttc gccagccata atctggatca ggaagtcttc cttatccata
     7741 tagtccgcga tatccagatc caccaggttc acaaacttgg tgccgtcttt caggttgtcc
     7801 accaccagaa tatcggtgat gcctttatca ttcagggctt taacgatgtt gcttccgata
     7861 aagcccgcgc cgccggtaac gatgatcata actgtaacct tcgaattatg gagtcagaga
     7921 caatctcaga cacgaatact tctatcatat cactacatga cgttggcttc agccattcac
     7981 caaaagtgag ggggctggac gttatttatc ctgaattttg cagaagtgtt aacgcgtcat
     8041 ctcgtcgcaa cctataagtt tgggtaatat gtgctggaat ttgccctgtc tggagaatcg
     8101 caatgcgtgg agaattttat cagcagttaa ccaacgatct ggaaaccgca cgggcggaag
     8161 ggttgtttaa agaagagcgc attattacgt ctgcgcagca agcagatatc actgtggctg
     8221 atggaagcca cgtcattaac ttttgtgcca ataactatct cggactggcg aatcatccgg
     8281 atctgattgc ggcggcaaag gcgggaatgg attctcacgg tttcggcatg gcttcggtgc
     8341 gttttatttg cggcactcag gacagccata aagagcttga acaaaaactg gcggccttcc
     8401 tggggatgga agatgcgatt ctctactctt cctgctttga tgctaacggt ggcctgttcg
     8461 aaacgctgtt gggcgcggaa gacgccatta tctccgacgc actgaaccac gcgtctatta
     8521 ttgatggtgt gcgtctgtgc aaagctaaac gctatcgtta tgccaacaac gatatgcagg
     8581 agctggaagc acgtctgaaa gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca
     8641 ccgatggtgt gttctcgatg gacggcgtga ttgccaacct gaaaggcgtt tgcgatctgg
     8701 cagataaata tgatgccctg gtgatggtag atgactcaca cgcggtaggt tttgtcggtg
     8761 aaaacggtcg tggttcccat gaatactgcg atgtgatggg gcgggtcgat attatcaccg
     8821 gtacgcttgg taaagcgctg ggcggggctt ctggtggtta taccgcggcg cgcaaagaag
     8881 tggttgagtg gctgcgccag cgttctcgtc cgtacctgtt ctccaactcg ctggcaccgg
     8941 ccattgttgc cgcttccatc aaagtgctgg agatggttga agcgggtagc gagctgcgtg
     9001 accgtctgtg ggcgaacgcg cgtcagttcc gtgagcaaat gtcggcggcg ggctttaccc
     9061 tggcgggagc cgatcacgcc attattccgg tcatgcttgg cgatgcggta gtggcgcaga
     9121 aatttgcccg tgagctgcaa aaagagggga tttacgtcac cggtttcttc tatccggtcg
     9181 ttccgaaagg tcaggcgcgt attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa
     9241 ttacgcgtgc agtagaagca ttcacgcgta ttggtaaaca actgggcgtt atcgcctgag
     9301 gatgtgagat gaaagcgtta tccaaactga aagcggaaga gggcatctgg atgaccgacg
     9361 ttcctgtacc ggaactcggg cataacgatc tgctgattaa aatccgtaaa acagccatct
     9421 gcgggactga cgttcacatc tataactggg atgagtggtc gcaaaaaacc atcccggtgc
     9481 cgatggtcgt gggtcatgaa tatgtcggtg aagtggtagg tattggtcag gaagtgaaag
     9541 gcttcaagat tggcgatcgc gtttctggcg aaggccatat cacctgcggt cactgtcgca
     9601 actgtcgtgg cggtcgtacc catctgtgcc gcaacacgat cggcgtcggc gttaaccgtc
     9661 cgggctgctt cgccgagtat ctggtgattc cggcgttcaa cgctttcaaa atccccgaca
     9721 atatctccga tgatctggct tccatttttg atcccttcgg taacgccgtg cataccgcgc
     9781 tgtcgttcga tctggtgggc gaggatgtgc tggtctctgg tgcaggcccg attggtatta
     9841 tggcggcggc ggtggcgaaa cacgttggtg cacgttacgt ggtgattact gatgttaacg
     9901 aataccgcct tgagttggcg cgtaaaatgg gtatcacccg tgcggttaac gtcgctaaag
     9961 agaacctcaa tgatgtgatg gcagaactgg gcatgaccga aggctttgat gtcggtctgg
    10021 aaatgtccgg tgcgccgcca gcgtttcgta ccatgcttga caccatgaac cacggcggtc
    10081 gtattgcgat gctgggtatt ccaccttctg acatgtcgat agactggacc aaagtgatct
    10141 ttaaaggctt gttcattaaa ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga
    10201 tggcggcgct gattcagtct ggcctcgatc tttcgccgat cattactcat cgtttctcta
    10261 tcgatgattt ccagaagggc tttgacgcca tgcgttcggg ccagtccggg aaagttatcc
    10321 tgagctggga ttaacacaaa caagggctgg tattccagcc ctttttcctg aggataatcc
    10381 gttaaatatg taaaatcctg tcagtgtaat aaagagttcg taattgcgct gatctcttat
    10441 atagctgttc tcattatctc tctaccctga agtgactctc tcacctgtaa aaataatatc
    10501 tcacaggctt aatagtttct taatacaaag cctgtaaaac gtcaggataa cttcagaggt
    10561 cgtcggtaat ttatgatgaa cagcaccaat aaacttagtg ttattatccc gttatataat
    10621 gcgggcgatg atttccgcac gtgtatggaa tctttaatta cgcaaacctg gactgctctg
    10681 gaaatcatta ttattaacga tggttcaacg gataattctg ttgaaatagc aaagcattac
    10741 gcagaaaact atccgcacgt tcgtttgttg catcaggcga atgctggcgc atcggtagcg
    10801 cgtaatcgtg ggatcgaggt ggcgacgggc aaatatgtcg cttttgtcga tgctgacgat
    10861 gaggtctatc ccaccatgta cgaaacgctg atgaccatgg cgttagagga cgacctcgac
    10921 gtggcgcagt gcaacgctga ctggtgtttt cgtgaaacgg gagaaacctg gcaatccatc
    10981 cccaccgatc gccttcgctc aaccggcgta ttaaccggcc cggattggtt gcggatgggg
    11041 ctttcttccc gccgttggac tcacgttgtc tggatggggg tttatcgccg cgatgttatt
    11101 gttaaaaata acattaaatt tattgccgga ttacatcatc aggatattgt ctggacaaca
    11161 gaatttatgt ttaatgcgct gcgtgcgcga tataccgagc aatcgttata taaatattat
    11221 ctgcataata cgtcagtgag tcggttgaat agacaaggga ataaaaacct taattatcaa
    11281 cgtcactata ttaagattac ccgcctgcta gagaaattaa atcgaaatta tgccgacaaa
    11341 attacgattt atccggaatt tcatcagcaa ataacttacg aagcattgcg tgtttgccat
    11401 gcggtgcgca aagagccgga tattattacc cgccaacgga tgattgccga gatatttact
    11461 tccggtatgt ataagcgcct gattaccaat gtgcgcagcg tgaaggtggg ttatcaggcg
    11521 ttactgtggt ctttccgctt atggcaatgg cgcgacaaaa cgcggtcgca ccatcgcatt
    11581 acgcgtagcg cctttaattt acgctagcgt taagttttgc cgcttcaggc tgtttgcccc
    11641 agccttgcca ctgatgttgg aagtaaacga tcagcgtgct ctggctgatg ctttcgctta
    11701 atacttcaaa gaagcgatta gcataaacag gttctatcgg tttcttcggt ttgcagagct
    11761 tcacgccacg gaacggatta cgcggtgtgt cgggcacagt gtttttcggc ggtgtagacg
    11821 tatcaacctg cggttcattc agcaagctgc tggctttcac cagcgtaatg tctggcggca
    11881 ggttataaac catctgttgt agcacgcgta ccgttgaagg gtgaggatgc ccaatggcaa
    11941 tggttgaacc gttgcgacgc gccagatcaa ttgcgcgatt gaactgcaca cggatgtccg
    12001 cttcattttg cgaatcgtcg aggaacactt tccgtttgat caccttcacg ccagtgcctt
    12061 gcgctgcgcg catcgcctgg gtattaccga tggttacgct gtcgaggaag taaagattgt
    12121 aacgctccag cgcctgcatc actttctgca taccaaacag gttagaggtc atcttgctgc
    12181 ccatgtggtt gttgatcccc acggcatagg gcacgttatt gaccgcacta cgaataatgc
    12241 gctcaatttc gtcgctgctc atctccgggc gtagcgtatt tttctccagc ggctgtttac
    12301 tcagtggtgc catcggtaga tgaatcaaca cttcgtgacc gctgttatgc gctttggttg
    12361 ccatctctct ggcgtgcggt gaatcgggta atacagcgac ggagatagcg gaaggcatcg
    12421 ccagcacctg gttttcgttg tgcgggcgat acccaaaatc atcaatgacg atggcaagct
    12481 tgccagcaat tacgggggag gagagcgcca acagagcggc aaatgcaaga acgttacgac
    12541 gaaatggaaa caaaacttat cttcccaacc acggctgtgg attgaccgcc tgaccctggc
    12601 ggcgaatttc gaaatagagt gaaggccgac cctgaccgcc actgctgccc accagtgcaa
    12661 tcggctggcc cgcgcgaacc tgcgaaccaa cgctcaccag tgcgctctga ttatagccgt
    12721 aaagactcat gtcgccttta ccatgctcaa ccaccaccac cagaccgtag ccttgcagcc
    12781 agtcagccag aatcacccga ccgtcggcaa tggctttaac ttcagtacct tcagaggcac
    12841 cgataaccat tcctttccag cgtaattcac cctgtagctg ctcgccatag cgatgcagcg
    12901 tcggcccgcg aactggccag aatgcctgac cacgcggcgc acccaggcca ccagttcggg
    12961 acatcagcga tttttcgctt tcggtcggtt tgtaggtggt gcctttgcgc gtcgcttctt
    13021 tctggcggtc gcgaaccgcc tgggcctcac gcgcttctcg ttcagcacgc gctttcgccg
    13081 cggcttccgc acgggcaatg ctgttacgca gacgggattc gttggcgcgc agctcgctca
    13141 actgttgctg accttgctgg atggaagact ccagccctgc cagcgtcttt ttacgctcgt
    13201 tcagcgcctg ggtcagcttc gcctgttggg cgcgctgctc atacaacagc gtttgttgct
    13261 cgctctgttt ctcttccagc tcggcgcgtt gcatagcgac ttcttcacgc gtttgtttca
    13321 actgagcaat ggtttcttgt cgcgcctggt tgagatagcc gaaataagcc tgtaaacgct
    13381 gtccacgctg gctttcttca ccgctgagaa tcagctgaat accggtatgc tcgccctgac
    13441 ggaatgcggc atctagttgt gcggcgaggc tgcgctcctg ggcggctttt tgctgctcca
    13501 gtttggcaat cgacgcgttc atctcatcaa tctgtttatt cagttgattg agcgtgtttt
    13561 gcgtttcacg cagcttacgg gtggcttccg agatcgcttc ttcctgcttt ttcaattgtg
    13621 cgagcaggct tgcgcgttgt tgttgctttt ggcgtaccgc gcgctctttc gcggcgatat
    13681 cggcctgaat agatttgagt tggtcacgct catccgcgtg ggcggaaaag gcgcacaaca
    13741 atacgccagc gctaagaacg ctggcgtaga tgatgggcct gattgcaaac ctgcgcggtt
    13801 tcacggcccg tgtcatggta ttaatcgcct ttcccctcat ggggagggat tattccacga
    13861 tgaacagcgg cttaccagtc atctcttgcg ggatttccat acccatcagc gacaacatgg
    13921 tcggcgcgat atcagaaagt ttgccgcctt caaccgcttt cacgttctta tcgccaacgt
    13981 aaatcagcgg aactggcagg ttggtatgtg ccgtgtgtgc ctgacccgtt gccggatcgc
    14041 gcatctgctc agcgttaccg tggtcagcgg tgatcagcag ttgtccaccc acggactcaa
    14101 ccgctttcgc gacttcttcc acgcagtgat ccagcgcttc aaccgcttta accgccgctt
    14161 ccatcactcc ggtgtgacct accatgtcgc cgttcggata gttacagata atggtgtcgt
    14221 atttgccgct cttgattgcc gcaaccagtt tttcggtcag ctctgcggag ctcatttccg
    14281 gttgcagatc gtaggtagcc actttcggtg agttgatcag aatgcgatct tcgcctttga
    14341 acgactcttc tacgccaccg ttgaagaaga aagtaacgtg ggcatatttt tcggtttcgg
    14401 aaatacgcag ctgagttttg tcgtttttcg ccatccactc gccgaaggtg ttaaccaggg
    14461 aagcgggtgg gtaagccact gcggttttga tgtcggcagc gtattcggtc agcatcacga
    14521 aatcgacgtt aaccactttc ttacgcgcga agccgtcaaa atcggcattt acgaatgcac
    14581 gagtgatttc acgtgcacgg tcagcacgga agttcatgaa aatcagcgca tcgccgtctt
    14641 ccattgccgc gtctggctga ccttctgcac gaataacggt cgctttcacg aattcgtcgt
    14701 tttcgtcgcg agcataagca gcctgcaaac cagcaacggc ggtatcggcc tggaactcgc
    14761 cctgcgccag agtcagcagg tcataagctt tttcaacgcg atcccaacgg ttatcgcggt
    14821 ccatggcgta gtaacgacca atgatggacg ctacgcggcc tttgcccagt gcggcaaatt
    14881 tctcttcgaa ttttttcagc gaggattcag cactgcgcgg cggagtgtcg cgaccgtcaa
    14941 ggaatgcgtg caggtagatt ttttctgcgc cgcgttcagc tgccagttct accatcgcca
    15001 tgatgtgatc ttcgtggctg tgtacaccac ctgcggagag cagacccata atgtgaactg
    15061 ctttgcctgc gtttttcgct ttatctaccg cgccagtcag taccgggtta gcgaagaaag
    15121 cgcgatcttt gatttcaacg tccagacgag tcaggtcctg atacacgatg cggccggcac
    15181 ccaggttaac gtgacctact tcggagttac ccatctgacg gtcaggcaga ccgacttcca
    15241 gaccggaagc gtcgatcagg gtatgcggac gattggccca cagtgcatcc attaccgggg
    15301 ttttagcact aaaaatggcg ttatcctgct gttcttcacg atagccatag ccatccagaa
    15361 tcaccagtac cataggtttt ttagaaaccg acattgcgac aacctcatac tcaagagtca
    15421 aaatttgcgt aattttacta cagctgaatc gataaaatag cctctgaaga tcaaaatata
    15481 cgaccgctcg cagcaagccg atgccagatt tgcgcttttt tttcgtcgcg ccccgcaaaa
    15541 ttgcattcca gttaacgcgc tggctgtatt tgctgcaccg cgcaggtata ctcctttcct
    15601 ggttttttta atcactacgt cgggagttgt taccccccat gcaagaaatt atgcaatttg
    15661 ttggccgtca tcccatactg agtatcgcct ggatcgcgtt actggtggcg gttattgtga
    15721 ctacgtttaa gagcctgacc tcgaaagtga aggtgattac tcgtggtgaa gctacgcgtc
    15781 tgatcaacaa agaagatgct gtggttgtgg atttacgtca gcgtgatgac ttccgtaaag
    15841 gccatatcgc aggttctatc aacctgttgc cgagcgaaat caaagccaac aatgttggtg
    15901 agcttgagaa gcacaaagac aaaccggtta tcgtggtaga cggttctggc atgcagtgcc
    15961 aggagcctgc aaacgcgctg acgaaagctg gttttgcgca agtattcgtg ctgaaagagg
    16021 gcgttgctgg ctgggctggc gaaaacttac ctctggtgcg cggcaaataa tttacctgtc
    16081 gtctttcatg atgcaggccg ttagctgcaa ttaggccccc agcgacttat tgttataagc
    16141 ttctggggac actccatttt gcagccgccc tgcaacatga aatttcaggg taaaaaaaca
    16201 ggagctaagt tatggccaat gttgaaatct ataccaaaga aacctgcccg tattgccatc
    16261 gtgcgaaagc actgctgagc agcaagggcg tgagttttca ggagttgccg atcgatggca
    16321 acgccgctaa gcgtgaagag atgatcaaac gcagcggtcg caccacggtt ccccagattt
    16381 ttattgacgc acagcacatt ggcggctgtg atgacttgta tgcattggat gcacgtggtg
    16441 gactggatcc cctgctgaaa taacgtgtga acgttggcat tacattgcgc agtatttaag
    16501 gacaacactt aagggttttc tacacatgtc agaacaaaac aacaccgaaa tgactttcca
    16561 gatccaacgt atttatacca aggatatttc tttcgaagcg ccgaacgcgc cgcacgtttt
    16621 ccagaaagat tggcagccag aagttaaact tgatctggat acggcatctt ctcaactggc
    16681 agatgacgtt tacgaagtgg tactgcgtgt taccgtaacg gcctccctgg gcgaagaaac
    16741 cgcgttcctg tgtgaagttc agcagggcgg tattttctcc atcgcgggta tcgaaggcac
    16801 ccagatggca cattgcctgg gtgcatactg cccgaacatt ctgttcccgt atgctcgtga
    16861 gtgcatcacc agcatggtat cccgcggtac attcccgcaa ctgaaccttg cgccggttaa
    16921 cttcgatgcg ctgttcatga actatttgca gcagcaggct ggcgaaggta ctgaagaaca
    16981 tcaggatgcc tgatgaacca acgtaatgct tcaatgactg tgatcggtgc cggctcgtac
    17041 ggcaccgctc ttgccattac cctggcaaga aatggccacg aggttgtcct ctggggccat
    17101 gaccctgaac atatcgcaac gcttgaacgc gaccgctgta acgccgcgtt tctccccgat
    17161 gtgccttttc ccgatacgct ccatcttgaa agcgatctcg ccactgcgct ggcagccagc
    17221 cgtaatattc tcgtcgtcgt acccagccat gtctttggtg aagtgctgcg ccagattaaa
    17281 ccgctgatgc gtcctgatgc gcgtctggtg tgggcgacca aagggctgga agcagaaacc
    17341 ggacgtctgt tacaggacgt agcgcgtgag gccttaggcg atcaaattcc gctggcggtt
    17401 atctctggcc caacgtttgc gaaagaactg gcggcaggtt taccgacagc catttcgctg
    17461 gcctcgacgg accagacctt tgccgatgac ctccagcaat tgctgcactg tggcaaaagt
    17521 ttccgcgttt acagcaaccc ggatttcatc ggtgtgcagc ttggcggcgc ggtgaaaaac
    17581 gtcattgcca ttggcgcggg gatgtccgac ggtatcggtt ttggcgcgaa tgcgcgtacg
    17641 gcgctgatca cccgtgggct ggctgagatg tcgcgtcttg gcgcggcgct gggtgccgat
    17701 cctgccacct ttatgggcat ggcggggctg ggcgatctgg tgctgacctg taccgacaac
    17761 cagtcgcgta accgccgttt tggcatgatg ctcggtcagg gcatggatgt acaaagcgcg
    17821 caggagaaaa ttggtcaagt ggtggaaggc taccgcaata cgaaagaagt ccgcgaactg
    17881 gcgcatcgct tcggcgttga aatgccaata accgaggaaa tttatcaagt attatattgc
    17941 ggaaaaaacg cgcgcgaggc agcattgact ttactaggtc gtgcacgcaa ggacgagcgc
    18001 agcagccact aaacccaggg aacctttgtt accactatga cccggcccgc gcagaacggg
    18061 ccggtcatta tcttatcgtg tggagtaagc aatgtcgtgt gaagaactgg aaattgtctg
    18121 gaacaatatt aaagctgaag ccagaacgct ggcagactgt gagccaatgc tggccagttt
    18181 ttaccacgcg acgctactca agcacgaaaa ccttggcagt gcactgagct acatgctggc
    18241 gaacaagctg tcatcgccca ttatgccggc tattgctatc cgtgaagtgg tggaagaagc
    18301 ctacgccgcc gacccggaaa tgatcgcctc tgcggcctgt gatattcagg cggtgcgtac
    18361 ccgtgacccg gcggtggata aatactcaac cccgttgcta tacctgaagg gttttcatgc
    18421 tttgcaggcg tatcgcatcg gtcactggtt gtggaatcag ggccgtcgcg cgctggctat
    18481 ttttctacaa aaccaggtct ctgtaacgtt ccaggttgat attcacccgg cagcaaaaat
    18541 tggtcgcggt atcatgctcg accacgcgac gggcatcgtt gtgggtgaaa cggcggtgat
    18601 tgaaaacgac gtatcgattc tgcaatctgt gacgcttggc ggtacgggta aatctggtgg
    18661 tgaccgtcac ccgaaaattc gtgaaggcgt gatgattggc gcgggcgcga aaattctcgg
    18721 taatattgaa gttgggcgcg gcgcgaagat tggcgcaggt tccgtggtgc tgcaaccggt
    18781 gccgccgcac accactgccg ctggcgttcc ggcacgcatt gtcggcaagc cggatagcga
    18841 taaaccgtca atggatatgg atcagcattt caacggcatt aaccatacgt ttgagtatgg
    18901 tgatgggatc tgatgtcctg tgatcgtgcc ggatgcgatg taatcatcta tccggcctac
    18961 agtaactaat ctctcaacac cgctcccgga taccccaact gccgccaggc ttcatacacc
    19021 acgaccgaca ccgcattaga caggttcatg ctgcggctgt ccggcaccat tggaatacga
    19081 attttttgtt cagtgggcag ggcatcaaga atgctcgctg gcaggccgcg tgtttccggg
    19141 ccgaacatca gataatcgcc atcctgatag cttacggcgc tgtgagcagg cgtacctttc
    19201 gtggtgaggg caaacaggcg ctggggattt tctgcttcga ggaacgcgcg atagtcatga
    19261 tgacgcgtaa cggcggtaaa ctcgtgatag tccagccccg cgcggcgcag gcgcttatcg
    19321 tcccaggcaa atcccattgg ttcgatgata tgcagacgaa agccggtatt agcgcaaaga
    19381 cggatgatgt tgccagtatt tggcggaatt tctggttcgt aaagtacgat gtttagcata
    19441 ctgccccctt agtgcggggg caggatagca gaaaattggt gattggtacg gccttttccc
    19501 ctcaccccca actatgccgc attccctttc gccatcggag ccagtgccgc cggcaactct
    19561 ttacccagcc cctgcaccag cgaatcttgc gtaatttcgc taatcgattt cgcgccagtc
    19621 agcgtcattg ccactttcat ctctttttcg atcagattca gcagatttgc cacgcccgcc
    19681 tggcccgctg ttgccagcgc atacaggaaa gcacgaccca gcagtacggt gtcggcaccg
    19741 agcgcaatca tacgcacgac atcaagcccg ttacgtattc cgctatctgc cagaatggca
    19801 atatcacctt tcaccgcatc cgcaatagcg ggcaatgcac gagcggaaga gagtacaccg
    19861 tccagctggc ggccaccgtg gttagaaacc acaatcccat cagcaccaaa acgtactgca
    19921 tcgcgcgcat cttccggatc gaggatccct ttgatcacca tcggaccatc ccagaattcg
    19981 cggatccact caaggtcttt ccatgagatg gacggatcga agttattccc cagccagccg
    20041 atgtaatctt ccagtccggt cggtttgccg aggtaagccg agatattacc taaatcatgt
    20101 ggacgaccgt tcaggcccac atcccacgcc cattgcggat gcgtcaccgc ctgcaagtaa
    20161 cggcgcattg ccgcatttgg gccgctcata cccgagtgtg catcacggta acgcgcgccc
    20221 ggtgtcggca tatccacggt gaaaaccagt gtcgagcagc ctgccgcttt tgctcgctcc
    20281 agtgcgttac gcataaagcc gcgatcgcgc agcacataaa gctggaacca catcggacgt
    20341 ttaatggctg gagccacttc ttcaatcggg caaacggaaa ccgtcgagag agtaaacgga
    20401 atgccatgcg catccgcggc tttggcagcc tgaacttcgc cgcgtcgcgc atacatgcca
    20461 cacaaaccta ccggacctag cgccaccggc atcgacaatt tctcattaaa cagcgtcgtt
    20521 tccaggctta agtcagacat gtttttcaga atacgctggc gcagcgccac ttctgacaaa
    20581 tcttccacgt tgcggcgcag cgtgtattca gaatatgcac ccccatccat atagtggaac
    20641 aggaacggcg gcagaatgcg ttgtgctgcg gcgcgataat cgctggctgc ggaaataatc
    20701 atgcgttttt ctccctcgaa tgctcattat ggtcaccagg caggcgggta atgcgtgcat
    20761 ggcgagcttg atcttcatcg aatcgtttca tggtggtgtg aacaaagcta aggtgcgcca
    20821 tcattgcttt acgcgccccg tcagcatcac cggcaaaaat ggcgtcaatg actgcctgat
    20881 gttgttcggt cagctgtgaa aaaaccgggg gtaccagata catccgctgg cggctatgct
    20941 tcacagagga ttgcaggaca tcgaagaaac cgcgcatggt ttgcagcagc acgatgttat
    21001 gtgaggcttc ggcaatcgcc agatgaaaac gaacgtccgc ttgtgaggcg agatccgggt
    21061 cttcacttag cgttgcttca aagcaaagct gaattttctc tttttcgcct ggtgtggcgc
    21121 gcattgccgc atgccacgcg gtgctggctt caatggcgta gcgggcttcc agaatatcga
    21181 aactgtaatc cggatcatcg gccatcagcg ttttcagcgg ctggacgatg ttttgctccg
    21241 accatgtatc atgacgccag cgaataaacg tcccgccgcc acgtcgactg agcagcacac
    21301 cttcactgac cagtttcgcc agcgcctcgc gcagtgaatt tctcgacacg ccgagttgca
    21361 tcgccagttg acgttcagcg ggcaacttca tgcccgcttc caggtttttt tcatcaatca
    21421 gcgcccgcac acgatcggca acctcgtctg acaggcgtct gggtaaaaca atcattaagg
    21481 aatcatccac gttaagacat aagcctgaag cgtggtgatc acgcccacca tacaggtgaa
    21541 gatcaggctg tgtttgacag taaagcggaa caaatcagac tctttgccca ccaggcctac
    21601 cgccgcacag gcgatagcga tagattgcgg agagatcatc ttaccggtga caccaccggt
    21661 ggtgttggcg gcaaccaaca acaggtcaga aacgccaatt tgttgtgctg cagtggcttg
    21721 cagggcggcg aacagggcgt tagatgaggt atccgatccg gtcaggaaga caccaagcca
    21781 gccgaggaac ggcgagaaga aggtgaatgc atggccggtg tgcgccagtg ccagcgccag
    21841 cgttgatgat agtccggaat agttcgagat aaaggcgaac gccagcacca taccgatgga
    21901 gtagataggc agagccagtt ctttcagcgt gctgccgaag gtgctgatag cgtcagatgg
    21961 tttcatcttc agccagacaa ttgagagcag ggcagcaaaa agaatggcgg taccggtggc
    22021 agagaaccag tcaaacttaa acacggcggc atacgctgtc gcttcgctga ccactggcgg
    22081 catacgggca accagtttat cgaggtacgg caccggaatg ttgatcaccc actcatacag
    22141 cgcaccgcct gaagcgaaca gggctttaaa cggcgggata ctccacagcg ttacggtggc
    22201 ggtcaggaac aggaacggtg tccaggcgcg aagcacctga cctgcggtgt aaccggtgtg
    22261 agccagcgtc atatcaacct gtgacgcccc caagtcaccg aaacggaata cgcgcactgg
    22321 ttgccagcgt ttgaggaaca gcgtcaggca gagcagtgat accagcgaag agataatgtc
    22381 tggcagttcc ggcccaatga agttagagct gaggtattga gcaatggcaa acgaaccacc
    22441 tgccaccacg accgcaggcc acgtttcttt gattccgcgc caaccgtcca taatcgccat
    22501 gatccagaac agcacgataa tggtcataaa cggcaactga cgccccacca tctgaccaat
    22561 ctcaaagctg tcgatgcctg ttacctgccc ggcgaccaga atcggaatac ccatcgcacc
    22621 aaatgccact ggcgcggtgt taacaatcag gcacagtccg gcggcgtaga gtggtttaaa
    22681 gcccagaccg accagcaatg cggcggtaat tgctaccgga gcgccaaagc ctgcggctcc
    22741 ttcaaggaaa gcgccgaaac agaaaccgac gatcagcatt tgcaggcgct ggtcaggggt
    22801 tatcgaaaga atagacgagc ggatgatgtc aaactgcccg gttttcaccg agatcttata
    22861 gacgaacact gccgcaataa tgatccacgc gatgggccac aaaccgtaga agaagccata
    22921 aatcaccgag gccagcgcgt tagcgaccgg cattttatag aacagcaaag ccacggcaag
    22981 ggcgattgcc accgtccatg aggcggcgac gtatcctttc agtttgagct taatcagcgc
    23041 aaaaaagaaa aacaggatgg gaagcgatgc tatcaggctg gaaagccaga tattcccggc
    23101 gggatcgtag ttttgttgcc agagattcat tgcaggtctc ctgcagtcca cacacgccgt
    23161 gtaatgagtc tgttgctcat ctccttgtca cacgttgtgt aaaagtggca ctgccaattg
    23221 tgttgtgtag ggataatgac aatgaatagt taactagatg ttatgtattg gcaacggtga
    23281 tgtgagtttt aattggggaa ttgtgaacca gaggtcagat caatgaagaa ttggtagggc
    23341 caattctggg agagaggtaa tgaagaaaag ggcaattccc atcagagaat tgccctcaga
    23401 ttgttgatct ggtgcgcctt atcttgccgg atgcggcgtg aacgccttat ccggcctaca
    23461 gggtagtgct gaatcacccc gtaggcctga taagcgtagc gcatcaggca atgtggcgtt
    23521 tacccattcg ttaatggatg attaccactg aataccggca ccgagcgcgg cggagtattc
    23581 accctggcta ttggtactac cttgtaattt gtagacccaa cgaccattgg cgctcaccat
    23641 cgacacacct aaagcaacag ccgattcacc gttgtaagta ccgccaccaa tagaggccat
    23701 gctggcacct ggcgtgtaag cctgcggcag accggtcatc gccattgcag aagcgatacc
    23761 accgctcaac ttgctttcgg ttttggacag tttgttatcc atctcaacca tgcgctgatc
    23821 ggtgtattgc ttcgtttcct gcacgctttg cttcaactgc gcgtagttca ccgcgtcgtt
    23881 gttgttaacg ccagcggaaa cgttgctgat gcgagtcgtt ccgccattac caccgccgag
    23941 agtgatgttg ctgtaatcaa cagaaccgtc agctttggtg tcataccgca cgccgcctgc
    24001 ttcggaagac ttcaactgcg caacgttaac cgcgtcagta gcatttttac cagcggcaac
    24061 gttggtgata cggcgttggt tagtggaaga acctacggag atcgtatttt cttcgtttgc
    24121 cacagagcca gtacccagcg caacgctgtt atcagcggca gcaatagagc cagagccaat
    24181 cgcaacactg tctttaccct gcgcgttagc atctacgcca tcggtgttgg tcttgaagta
    24241 cttggtgcta ccggtgctta cgatatcgcc aataccgttt tcgatattgg tgacgcgggt
    24301 atccagattc gttacctgag tattcaggtt agtaatgctc tgggtattct gggaaacctg
    24361 cgcgtccgtt actttcaact gacccacgtt aaccgcatcg gtatccgccg accccgctgc
    24421 gacgttggtg atctgacgtt gaccgtcttc gctacctacg gagaattcac ccacagagtt
    24481 ctgcggtgca tccatgttgt aggcggtgta gccagtctga gcgccgcgag tggtctgaga
    24541 accattaccc attgcaatac tgtttgcatg gttggcgcgt gcgttagaac cgatagcaat
    24601 accgttgatg gcatctgcca gaaccagagt attcaggccg ataccaatac ccgcattacc
    24661 attgacgata gttttcgcgc ccatcgccag cgagtcttcc ccgacagcca gtgagtcttc
    24721 agccgttgag ttggcgtgga agtatttggt cggcgtggta gcgactgcac caatggcatt
    24781 ttgcaactgg cgaaccgtaa cggcgtcatg ggcttcggaa ccatcggcta cgttgatgat
    24841 ttgacgatat ttaccgtcat caccaataga cagcgcacca agtagttcgc catccgtggt
    24901 gtcataacca atcacaacac cttcttccga cacgctggtg tcacgagaac ctttaactat
    24961 tacacggctg gaaacggagc tgctacccag ggcaataccg gtatcaacgt cgctgtagct
    25021 gccctgaccg atggctacgc tgctatcgcc ttcggcaaca gagttataac ccacagccgt
    25081 agcgccaata cctgaagcgc tggcatcgtt gaacgctaag ccgttgtcgt tggtacgtac
    25141 atagttaata cccgcaccgt tatcttcgat gtaggtttcg ctggtgttac cggcaagctg
    25201 attgatgatt tcgctgttct gctggatcat catattggtg ttaaacagct gagaaccgtt
    25261 gatggcgtcg gtactgcctt cgccaatgtt accatcagca acatttgtga tgatgctggc
    25321 cttgccgtca tggctggcgt tgtaggcacc tgcgccgtca ttggccgttg catcccacag
    25381 cagagcgtta tcgtcgagcg catccagggc gtcaccgacg ttgttgtact cggcatttgc
    25441 aatggtgtaa gtcggcgcag tgatagaacc atcagcgtta acttctgcgt caccacccag
    25501 ggcatcggca atgtacttgt tggtggtata cagttgtgaa ccgttaatgg catcgctgct
    25561 ggtggcagag attgcaccgt tagcgacgtt agtgattaca ctggctgttt tatcttttcc
    25621 gtgagcggcg ctaaatgcac cattaccatc attggctgtg gcatcccaca acagagcgtc
    25681 atcgagcgtg gtatcgattg cttccagcgc gtcaccgacg ttgctgtaac tgccgccagc
    25741 gatagcataa gtcggtgcgg tgattgtgcc gtcagcattg acttctgcgt caccacccag
    25801 agtatcggca atgtacttac tggtgtcata gagctgtgag ccgttaatag cgtcgctgct
    25861 ggtagaggaa attgcaccgt ctgcaacatt agtaatgatg ctggccgtgt tatcttttcc
    25921 gcgaccggcg ctaaacgcac cgtcaccgcc cttggcggtc gcatcccaga gtagggcgtc
    25981 accgagtgat gtgctaaatg acgtgttaat ggctgccaga gcatcaccga cgttattgta
    26041 gtcagtacca ccaatagtgt aggttggccc ggttaataca cccgcatcgc tgatatcagc
    26101 accaccgcca aggtatgacg taaatgaatc agctaaacca taaagttgtg agccattaat
    26161 ggcgtcagtg ctatcagagg atacagtgcc tgccagtaag ttggtgattt tgctgtcagt
    26221 accgttggtg ccgtgtttgg cactgaatgc acccgccgta gcgttccaca gcagggaatc
//
LOCUS       DAZGTJ010000075        26007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000075 DAZGTJ010000000
VERSION     DAZGTJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.075"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHK8E_003635"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHK8E_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP0345850.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHK8E_003636"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHK8E_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP0345851.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHK8E_003637"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHK8E_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP0345852.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHK8E_003638"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHK8E_003638"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP0345853.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="ACHK8E_003639"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="ACHK8E_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP0345854.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="ACHK8E_003640"
     CDS             complement(8170..9063)
                     /locus_tag="ACHK8E_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP0345855.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHK8E_003641"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHK8E_003641"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0345856.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHK8E_003642"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHK8E_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP0345857.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHK8E_003643"
     CDS             complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHK8E_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP0345858.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHK8E_003644"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHK8E_003644"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP0345859.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHK8E_003645"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHK8E_003645"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP0345860.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="ACHK8E_003646"
     CDS             complement(14231..16495)
                     /locus_tag="ACHK8E_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP0345861.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHK8E_003647"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHK8E_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP0345862.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHK8E_003648"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHK8E_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP0345863.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHK8E_003649"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHK8E_003649"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP0345864.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHK8E_003650"
     CDS             complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHK8E_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP0345865.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHK8E_003651"
     CDS             complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHK8E_003651"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP0345866.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHK8E_003652"
     CDS             complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHK8E_003652"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP0345867.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23360..24052)
                     /locus_tag="ACHK8E_003653"
     CDS             complement(23360..24052)
                     /locus_tag="ACHK8E_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP0345868.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHK8E_003654"
     CDS             24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHK8E_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP0345869.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6304 a         7081 c         6325 g         6297 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg ccagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac ggatgtaata gtcagtaatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct ggtgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc ataatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat cagcaagccg tgcgcattgt agaacattaa
     6841 cgacaacaca ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgtga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccgcgctca gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cggttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctaatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc accaagctcg aatttaatca ataacagtct gccccgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc
     7801 gtagcaaaaa ccaatacggt gagtacagcc gaagtaggtg cccgagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcactctg gatttgccaa aagataccga agaagaagct
     7921 gcggcagtga aacaggcgat tggtgacgcc cctttcctgc tggtgacatc cgcgtctcat
     7981 ttgccgcggg cgatgatctt ttttcagcag gaagggttaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaac ccgtgggaac gggcaatccc ttctccggtc
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgtatctg gcagtggcta
     8161 aaaagttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 cgccagagaa gtatccatta gtcggaacag ttcgtattcc cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtgttcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatcaccc tgcacattaa cgatcacagt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaat cgttctgtac ctgactgatg atcggcgcgc gtcatacata cttcgccgcc
     9781 agcggcttca acagcgcggg caacatcctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtaacgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagctcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagcc ggcgcggcaa tcagtaaaga ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc acgccacgcc gccagtgccg gacgttttag ccggtagtaa
    10741 tctgccagcc actgttcgcg aaatataccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgcttgcga aacgagatcg cgctcatcat tccactcagg catgacacgg
    10861 tgagttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg tcgtttatgc ggcttccact cccaccagcc gctcgcccct
    10981 ttacgaggat gctcaaaatc ggctgaacgt actggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgccgc atgttcctgc atccaggcgt ctttatattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaaat cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatattg
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagcggacgc ataagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgtacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgccaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aactgacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc gcaaccatcg cggcgaggat caacggaacc gtgataaatg
    15301 tcaccagggg aattgcaaat agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacgatttg cacgggcata agcaggagtg tgattcccag ttgtaaatga atgagggaaa
    15421 caactgcacg caataccggc ggcagttgcc actccggaca gggaaaccac tggtaccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc gccagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cggtgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catgtcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagctg atcgcgctct gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcagcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgcg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc acggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagctgca
    19321 ttcgccactt cctgagtacg aatttcgcca ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg caccagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc
    20401 ttttgcatca gcatcgctta acgttgctgc tttataggca tttaaacctg agctgatagc
    20461 catattggta tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggccgc
    20521 aatcgccagc agtaatttag tattcttcat gtgaatcatc cgttgattaa tcatatataa
    20581 atattatgta tcaataactt acattgacaa gcgacgctac cattaatcaa aatgactcac
    20641 aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgttttg ataaagaaag atttggctat
    20701 ttattgcacg ttgttcattc aggctgcctg gctaatccgc gccagctgct cgaaataatt
    20761 cggaaatgtt ttggccgtgc atttgggatc aagaatcgtc actggtgtat ctgacaacgc
    20821 caccagcgag aaacacatcg ctatccggtg atcattgtat gtcgcgatct cggcaaagtt
    20881 cagcttttcc ggtggagtga tacgaatgta atcgtgcccc tcttctactt ccgcaccaac
    20941 tttacgcagt tctgttgcca tcgcaaacag gcgatcggtc tctttaacac gccagttata
    21001 gatattgcgc agcgtggtgg tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc
    21061 cgcatcggga atatggttca tatccatatc aatagcgttc agttcacccc gcgtgcagga
    21121 aatataatca tcgccccagc aaatggtcgc gcccattttt tccagcacat cagcaaagcg
    21181 aatatcaccc tgcatactgt tgcgtccaat gccggtcact tttacagtgc cgcctttgat
    21241 tgctgctgct gccaggaagt aagaagccga agatgcatcg ccttcgacca aataagtacc
    21301 cggagactga taagactgcc cgccttttac gacaaattgt tgatagtgct gattttcaat
    21361 ttcaacacca aacgtcttca tcatattgag tgtgatgtcg atataaggtt tagaaaccag
    21421 atcgccttta atacgaatca ccgtatcttc cggtgcaaga ggcgcagtca ttaatagtgc
    21481 ggtgaggaac tggctggaaa cggagccatc aacgtcaacg ttgccgccag taaagccgcc
    21541 ctgtaaacgc aatggcggat aattgtcttg ttccaggtaa gtgatcttcg ccccgcccag
    21601 gcgcagcgca tccaccagat gaccaatcgg gcgctctttc atacgcggct caccagtcag
    21661 cacaatatca ttgctaccca gacaaagagc tgccgccagc gggcgcattg ccgttccggc
    21721 gtttccgagg aacaactcca gggcaccttc tgcgtgtaat ggaccgccgt taccgataat
    21781 ttcgcaacgc gtacgatcgg ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt
    21841 cagcatatgg cgcacgtcat cgctatccag cagattggtt aatactgttt tgccgtgtgc
    21901 taatgccgcc agcaataaag cgcggttaga aacgctcttg gaaccaggca gattaatagt
    21961 gccatcgaca cgagcgatgg gttgtaacgt cagggattcc atgaactcaa ctctctacaa
    22021 cagaaataaa aaccccacag gctggctgtg gggattaagc aaaatttcgg cattagccgt
    22081 gacggcgttc gaactcaacc atgaagtctg tcagggcttt aacgccttcc agcggcatgg
    22141 cgttataaat agacgcgcgc attccgccga ccacacggtg acctttcagc gcatgaaggc
    22201 cagcagcaaa agactcttca aggaacaatt tgtcaagcgc actgtccgcc aactggaacg
    22261 gcacgttcat ccgcgaacgg ttagctttcg ccacgtcatt gcggtagaaa ttgctgttat
    22321 caatcacccc atacagcagt tctgcttttt gctgattgat tttatccatt gcagctacac
    22381 cgccgttcgc tttcagccat ttaaagacca gaccagacag ataccaggca aatgtcggcg
    22441 gcgtgttaaa catggagtcg ttatcgttga gaatggaata atccagaatc gacggacacg
    22501 cgatattcgc tttgcccagc aaatcttcac gaacgatgac gattgtcagg ccagccgggc
    22561 cgatattttt ctgcgcgcca gcgtaaatca cgccataacg gctgacgtca atcggacggg
    22621 aaagaatggt tgaagagaag tcggcggcga ccaccacatc tttgccgaag tctggcgttt
    22681 cgtcgatagc gataccgtcg atggtttcat tcgggcagta atgcatataa gcagcattat
    22741 cagagagttg ccattcgcgc attggcttaa ccgcgcgcag accatcaaca gtcactttgg
    22801 cgtcaaagac attaggcgtg cagtattttt tcgcttcttt aatggcactt gccgcccagt
    22861 aaccggcatc aacataatct gcggtggttt tatcaccgag aatattcagc ggaacagcag
    22921 caaactgacc gcgaccgccg ccatggcaga acaatacctt gtagttggag gggacattaa
    22981 gaagatcgcg aaaatccttc tcggcttcct ctgcaacctg aatgaactct ttgccacggt
    23041 gactcacttc catcaccgac gtaccaagac cgttccagtc gcgcagttcc tgttgagcct
    23101 gtttaagcac ctctgccggt agcattgccg gaccagaact aaaattgaag atttgagcca
    23161 tttcccctca ccacgttgcg ttgctattaa cccgcggtca atcgattgcg accacaggta
    23221 atacattatt cctgtggcta tcggttttat cattcagtga cacacatcgc aatgagtaaa
    23281 accgttgagg gcaaaaatgt ggcttgcatc acacaaaaga atctctcgga ttattagcaa
    23341 aaagccgatg tttttactgt cacgtcaccc gttttgcccg acttgccttc gtccattctg
    23401 gatttgcaca gcgcggacat aattgttttt cccccgcgtt catatgaatg atttcactgc
    23461 aacgaatgca ggcatagtcc accgactcgg gtaccacttt gtaacgctga gtacagtcac
    23521 tgggtaaaat aagtaagccc ggtttcacct tgtcatcttc aaagaaatag acactgattt
    23581 gcccgttggt ttcgagaatc gccagacgta cttgccccaa ctgctctaca ccgcgcaatc
    23641 gtagctccat aaagaactca aattccgtca tgttggagtt attgagtttc gaccaggcca
    23701 gctcgccatc ttcaataatg acaaccggct tgccttccag aagatcttcc agtttttcac
    23761 tgtgcgccat caaccacatt accaggcggt ataacaacgc cagaataata aagacgataa
    23821 gtaccgggac catcggcaca tcatcataaa acgccacatc tcccgccgct gatcccagcg
    23881 tcagaatgat taacacttca aaaagcgaca tctgccgcac accgcgtctt ccggtcattt
    23941 tgaggaacaa aaaaaccagt acaaaggtat aaagactacg tagtgccact tcgccaagga
    24001 aatcaaaagg cactttatca aatgccatac ggtggagatc gaacgccttc atttatatgc
    24061 tcttatcgtg gtttatccgc taaaagcata gtcaacagat catcggcatg gcggcggtga
    24121 tagcgccttt gagataaaac ccgtattatt gcgcgctttc cgtacgacta aagtgatttt
    24181 catgacgcaa acatttatcc ccggcaaaga tgccgcactg gaagattcca tcgctcgctt
    24241 ccagcaaaaa ctttcagacc tcggctttca gattgaagag gcctcctggc tgaatccggt
    24301 gcctaacgtc tggtctgtac atattcgcga caaagagtgc gcactgtgtt ttaccaacgg
    24361 taaaggcgca accaaaaaag cggcgctggc ttctgcactc ggtgaatatt tcgagcgtct
    24421 ctcaaccaac tacttttttg ctgatttctg gctgggcgaa accatcgcca acggtccgtt
    24481 cgtgcattat cccaacgaaa aatggttccc actgagcgaa aatgacgatg tgccagaagg
    24541 gctgctcgat gaccgtctgc gcgcatttta cgatccggag aatgaactgg ccggcagtat
    24601 gctgattgac ctgcaatccg gtaacgaaga tcgtggtatt tgcggtctgc cgtttacgcg
    24661 tcagtctgat aatcagaccg tttatattcc gatgaatatc attggtaacc tgtatgtttc
    24721 taacggtatg tccgctggca atacccgtaa cgaagcacgc gttcaggggt tgtccgaagt
    24781 tttcgaacgc tacgtgaaaa accgcattat tgctgaaagc atcagcttgc cggaaatccc
    24841 ggcagacgtg ctggcgcgtt acccagcggt ggttgaagcc atcgaaacgc tggaagcaga
    24901 aggtttccca atctttgctt atgatggttc gcttggcggc cagtatccgg tgatttgcgt
    24961 ggtactgttc aatcctgcta acggtacctg ctttgcctct ttcggtgcgc atcctgattt
    25021 tggcgtagca ctggaacgta ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt
    25081 ggatgtgttt actccgccaa ccttcgatga tgaagaagtt gctgaacata ccaacctcga
    25141 aacgcacttt atcgattcca gcggtttaat ctcctgggac ctgttcaagc aggatgccga
    25201 ttatccgttt gtggactgga atttctccgg cactacggaa gaagagtttg ctacgctgat
    25261 ggctatcttc aacaaagaag ataaagaagt ttatattgcc gattacgagc atctgggcgt
    25321 ttatgcttgc cgaattatcg tgcctggcat gtccgatatt tatccggctg aagatctgtg
    25381 gctggcgaat aacagtatgg gcagccattt acgtgaaacg attctttcgt taccaggcag
    25441 cgagtgggaa aaagaagatt acctgaacct catcgagcaa ctggatgaag aaggttttga
    25501 tgactttacc cgcgtgcgtg agctgttggg tctggcgacc gggtcggata acggttggta
    25561 caccctgcgt atcggtgaat taaaagccat gctggcgctg gctggtggcg atctggaaca
    25621 ggctctggtc tggaccgaat ggacgatgga gtttaactca tcggtattca gcccggaacg
    25681 cgccaactat tatcgctgcc tgcaaacgtt gttattactg gcgcaggaag aagatcgcca
    25741 gccgctgcaa tatctgaatg cgtttgttcg catgtacggc gcagatgccg tggaagccgc
    25801 cagtgcggca atgagcggcg aagcggcgtt ttatggtttg caaccagtag atagcgatct
    25861 gcacgcgttt gctgcacatc agtcgttgtt gaaggcctac gaaaagctgc agcgcgccaa
    25921 agcagcattc tgggcaaaat aaaatcaaat agcctacgca atgtaggctt aatgattcgt
    25981 ctgagttata ttacggggcg ttttttt
//
LOCUS       DAZGTJ010000076        25917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000076 DAZGTJ010000000
VERSION     DAZGTJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.076"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2784
                     /locus_tag="ACHK8E_003655"
     CDS             <1..2784
                     /locus_tag="ACHK8E_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0345870.1"
                     /translation="VNDGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDG
                     TVILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDI
                     AFNRLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFI
                     MDGGADISGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDW
                     ESRTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPV
                     KGTSAVGGASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRL
                     TSSALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLN
                     GSGEYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGG
                     EFHTLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNI
                     PLVTAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATR
                     QDAETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRS
                     TVNSSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGG
                     FSDNYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALF
                     DSGAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQA
                     ELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGY
                     QFDLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYN
                     VDNAVNASFRYSF"
     gene            complement(3352..6024)
                     /gene="rcsD"
                     /locus_tag="ACHK8E_003656"
     CDS             complement(3352..6024)
                     /gene="rcsD"
                     /locus_tag="ACHK8E_003656"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP0345871.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            6763..7893
                     /gene="ompC"
                     /locus_tag="ACHK8E_003657"
     CDS             6763..7893
                     /gene="ompC"
                     /locus_tag="ACHK8E_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0345872.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8005..9060
                     /gene="apbE"
                     /locus_tag="ACHK8E_003658"
     CDS             8005..9060
                     /gene="apbE"
                     /locus_tag="ACHK8E_003658"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP0345873.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            9134..10198
                     /gene="ada"
                     /locus_tag="ACHK8E_003659"
     CDS             9134..10198
                     /gene="ada"
                     /locus_tag="ACHK8E_003659"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP0345874.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            10198..10848
                     /gene="alkB"
                     /locus_tag="ACHK8E_003660"
     CDS             10198..10848
                     /gene="alkB"
                     /locus_tag="ACHK8E_003660"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP0345875.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            10924..12567
                     /gene="yojI"
                     /locus_tag="ACHK8E_003661"
     CDS             10924..12567
                     /gene="yojI"
                     /locus_tag="ACHK8E_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP0345876.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            12785..14431
                     /gene="mqo"
                     /locus_tag="ACHK8E_003662"
     CDS             12785..14431
                     /gene="mqo"
                     /locus_tag="ACHK8E_003662"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP0345877.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(14581..15090)
                     /gene="eco"
                     /locus_tag="ACHK8E_003663"
     CDS             complement(14581..15090)
                     /gene="eco"
                     /locus_tag="ACHK8E_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP0345878.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(15204..15368)
                     /locus_tag="ACHK8E_003664"
     CDS             complement(15204..15368)
                     /locus_tag="ACHK8E_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345879.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            15477..15971
                     /gene="napF"
                     /locus_tag="ACHK8E_003665"
     CDS             15477..15971
                     /gene="napF"
                     /locus_tag="ACHK8E_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP0345880.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            15961..16224
                     /gene="napD"
                     /locus_tag="ACHK8E_003666"
     CDS             15961..16224
                     /gene="napD"
                     /locus_tag="ACHK8E_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP0345881.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            16221..18707
                     /gene="napA"
                     /locus_tag="ACHK8E_003667"
     CDS             16221..18707
                     /gene="napA"
                     /locus_tag="ACHK8E_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP0345882.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            18714..19409
                     /gene="napG"
                     /locus_tag="ACHK8E_003668"
     CDS             18714..19409
                     /gene="napG"
                     /locus_tag="ACHK8E_003668"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP0345883.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            19396..20259
                     /gene="napH"
                     /locus_tag="ACHK8E_003669"
     CDS             19396..20259
                     /gene="napH"
                     /locus_tag="ACHK8E_003669"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP0345884.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            20256..20705
                     /gene="napB"
                     /locus_tag="ACHK8E_003670"
     CDS             20256..20705
                     /gene="napB"
                     /locus_tag="ACHK8E_003670"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP0345885.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            20715..21317
                     /gene="napC"
                     /locus_tag="ACHK8E_003671"
     CDS             20715..21317
                     /gene="napC"
                     /locus_tag="ACHK8E_003671"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP0345886.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            21330..21953
                     /gene="ccmA"
                     /locus_tag="ACHK8E_003672"
     CDS             21330..21953
                     /gene="ccmA"
                     /locus_tag="ACHK8E_003672"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP0345887.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            21950..22612
                     /gene="ccmB"
                     /locus_tag="ACHK8E_003673"
     CDS             21950..22612
                     /gene="ccmB"
                     /locus_tag="ACHK8E_003673"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP0345888.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            22654..23391
                     /gene="ccmC"
                     /locus_tag="ACHK8E_003674"
     CDS             22654..23391
                     /gene="ccmC"
                     /locus_tag="ACHK8E_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP0345889.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            23388..23597
                     /gene="ccmD"
                     /locus_tag="ACHK8E_003675"
     CDS             23388..23597
                     /gene="ccmD"
                     /locus_tag="ACHK8E_003675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP0345890.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            23594..24073
                     /gene="ccmE"
                     /locus_tag="ACHK8E_003676"
     CDS             23594..24073
                     /gene="ccmE"
                     /locus_tag="ACHK8E_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP0345891.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24070..>25917
                     /gene="ccmF"
                     /locus_tag="ACHK8E_003677"
     CDS             24070..>25917
                     /gene="ccmF"
                     /locus_tag="ACHK8E_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP0345892.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAG"
BASE COUNT         5887 a         6375 c         7346 g         6309 t
ORIGIN      
        1 gtgaacgacg ggcaccgtgt tgactggatg gtgaacggac ttgcgggaga cgcacttcat
       61 aaaattggta agggaacact ggtggtggcc ggcagtggtg aaaatcctgg caccctgaat
      121 acgggggacg gaacggtcat tctggcacag aaggctgatg ctgcaggcag ggtccgggca
      181 ttcagtgaag tcagtattgt cagcggacga ccagttgtgg tgctgcagga cagtcatcag
      241 attgaagggg accgcattcg ctggggatac cggggaggta cgcttgatat caacggaaat
      301 gacatagcct tcaaccgcct ggctgcggct gatgaggggg cagtactcac aagccgggcg
      361 cgtcctgcca cagtccgtct ggatttcagc cagtcagggc agaaggccgt gatgtggcat
      421 ggtcatttta ccggtaacct cagcgtcctg aacaacacgt catctgcggt ggattttatc
      481 atggatggcg gagcggatat atccggcagt tttacacagc agggcggggg actgtatatc
      541 caggggcatc cggttgttca tgctgtcagc agtgaggcgg ttgcgactgc attgcgaaag
      601 cagggtgata attctgttct gacgcagccg gtatcatttg ctcagaagga ctgggagagc
      661 agaacgttca gcatcggtca actgaaactg aagggggcgg cattcagcct ttcccgcaat
      721 gcgacactga cgggagatat tgatgctgat aatgccacca tggtgctggg aagtgactcg
      781 ctatatctgg atatgaagga cggcacgggg agcagcagtg cgccggttaa ggggacgtct
      841 gccgtgggcg gtgcatccgg tagcactaca tttcgcggga atgtgaatat gcgtcattct
      901 gccctgacgg tccgcgacca ttttactggc agtataacgg catcggacag ccggatagct
      961 gtgagttcag agaatgtgcg tctggaaggg aattcccgcc tgacctcttc tgcacttact
     1021 gtcagtgacg gcgggcgttt acatgtgaag gggggactgg agacagatgg tggtgtgaca
     1081 ctggacagag gaaccctttt ggtggacggg ggaagtgtca ggaatgatgt atatgaacgt
     1141 ctgctggcct ggtcagagga gcgtggcggg ctgaacggaa gcggtgagta tgattttatg
     1201 acgggggcag ctggtctcct gcggagttat gtcagaggga gtgcaggtaa tgtgaatctg
     1261 cagaatgcgg cctggatgat gacgggaaac tcatccgtga agcatcttga gtcatcggga
     1321 agtgctctgt atttcagcag gccgggcggg gagttccata ccctgacggc aggcagtatg
     1381 gatatcagcg acagtgttct ggtgatgcgg acggacctta accactcaga ccagcttcgg
     1441 gtgacagaat cgctgcgggg taagaataac ctgttgttgg ttgattttac tgagcgttct
     1501 gacgggcaga aggcgctgaa tatccctctg gtgacggcgc cggccggtac cggtgcggat
     1561 gtgttcagtg tgaaaacgcg tgataccggc ttcagtcata tcacacctgt tgtcagggct
     1621 gaacagggga cgggcggcac ggcctggcag ctgaatgttg tgcagccgga aactgccgca
     1681 gagcctgtgg cgacgcggca ggatgcagag acgccgaatc ctgtggaagc tgtatcaccg
     1741 ctcccttcac cggtttctgc tccgtcatcg gctccggagg cgtcagtgac ggatgtactg
     1801 accggagaga atgcggggga atcaggtgag taccgtgatg agacccggtg gctgctgaca
     1861 ggataccgga gtacggttaa tagctccgcg gtcagggatg cggggatgct gatgtcaatg
     1921 gggcaccgta acttcattaa tgaggtcaac aacctgaaca aacgtatggg tgacctgcgg
     1981 gacatcaacg gcgaggccgg tgcatgggcc cgtatcatga gcggaaccgg ctctgccggc
     2041 ggtggcttca gtgacaacta cacacacgtt caggtggggg cggataaaaa acacgaactg
     2101 gacggtctgg acctcttcac cggggtgacc atgacatata ccgacagcaa cgccggcagc
     2161 gacaccttca gtggtaaaac gaagtcagtg ggtgcgggtc tgtatgcttc agccctgttt
     2221 gattccgggg cctatatcga cctgattggt aagtatgttc accatgataa tgagtatacg
     2281 gcgtcctttg ccggactcgg cacaaaagac tacagctctc attcctggta tgccggtgct
     2341 gaagtgggtt accgctatca tgtcacagag gatgcctgga ttgagccaca ggcggaactg
     2401 gtctatggtg ctgtctccgg gaaacagttc agctggaagg accagggaat gaacctcacc
     2461 atgaaggata aggactttaa tccgctgatt ggccgtaccg gtgtggatgt gggtaaatct
     2521 ttctccggta aggactggaa agtgacggca cgtgctggtc ttggctatca gtttgacctg
     2581 ctggcgaacg gagaaacggt actgcgtgat gcatccggag agaagcgtat taagggcgag
     2641 aaagacggcc gtatgctgat gtctgtgggt ctgaacgcag aggtccgtga caacatccgt
     2701 tttggtctgg agtttgagaa atcagccttt ggtaagtaca acgttgataa tgctgtcaac
     2761 gccagcttcc gttattcatt ctgacggttg ttactgaagg ggattatggc ctgacggctc
     2821 cctgtaaata aagacaggca cagaagattg tgagttactc agtgttccac caaaaagtaa
     2881 ggtcgcaact ctgtgcctgc ttcgtaagtg tgcagtgagg gccatatggt tataactgaa
     2941 gcatccgacg acgtagtgtc taccagagtg tcagatatgc aggaaaatat gagttccgac
     3001 agaaatgcag taaaaaaagg agtctcgcac tcccctccat aaaactccag ggctgtaagt
     3061 tgtattaaaa agctgatttt tctttagcga tacagacctc aataagtata acacccttca
     3121 gttactgtgg ttatgcacag gtgttatttt taacagtgtg gataaacagt gtcgattatt
     3181 cagattggtt atggggcatg tatgggacat ttttaatcgg cgaaatacgt cgaagttcgt
     3241 cgatatggta aacgaatcat ctatccaacc cttgaaaaac ggcgctcctg gacgatcttc
     3301 gtcgattttt aaaatgcaac aagtacgttg ttcatgtaat aggctacctt gctacagcaa
     3361 gctcttgaca taactgtcaa tgtcgctgat gtatttttct attcctggaa catccttctc
     3421 acgaatcaga tgttccagcg tttcacataa ctgcttgccg ggtaccagat ttagcatggc
     3481 aaatacgcct ttaagacgat gagccgtttg ggctaacgca gcaaagtcac tggtcgctgc
     3541 ttcagtatac agcctcttaa catcatccgg tactgtgtct acaaagagcg cataatagcc
     3601 gctggcatgg agttgcgcat tttcatctcc gcccagaggc gattctgtca cctcttcctg
     3661 cgccagttgc acttcaatta attgtaagac cgcttcctgc atagcgttgc tcatattgaa
     3721 gttgacgcac aattgaccag gcccaatttc ccgtacgcca gactcatcat cgcttaaaag
     3781 caagccagag gcagtaagat tagacggatt atccgttaaa aagatatcat aatcttgact
     3841 aattaatctt tcatcgggtg tgatacaggt tgcaccccaa ttttctaact ggcgagtgac
     3901 aatattccga atttctgccg aagtaacatc caccattacg cagacatcat ccagtaaacg
     3961 ctcttcttcc tcttcaactt ccgggtcagc tgcgagcatt ttgatatgca cagagtagcg
     4021 agtaccaagc ccatcccgcg ttttgatgtt taaatgaccg cccagtttac gcgccagttg
     4081 atcgctcagc cagaatgcca gcgggtccgc cttgccatag cgatcgtttt gggtctggtt
     4141 gataaacggg aagtgcaaat tatccatttc atgaatgctt acgccttctc cagtgtccag
     4201 aatacggaac gtcaggcggt cttcggagga ctcatcctga tcaacctcaa gggtgatttt
     4261 tcccaattgc gttgaggtca cggcatattg catcagtaac agcaaaatac gtcgtaaggc
     4321 atcgcgatcg ccgcggcgca tgtcgtgtgc tttcagatga ttgttaatca gcagttgcag
     4381 acctttacgc ttgatggcag gcaacactga aggcacaact tcatcaatta aatcctgcac
     4441 ggagaacagc accgtctcac ttttccagct atcgtccgca agcatgttcg ctaactgtat
     4501 ttcatcgacc agccgcacca gcacatccgc ctgattcgtc agttgtttgc cttccggggc
     4561 gttgagttta gccgcgctct ccgccaggga ctgtgcgggt tctttcagcg catcgccaat
     4621 gtttttcata aaggtcatcc gcccctgctg gtttttctca tacagacgct gcgcctgctt
     4681 gagtttcttg tttaccagca cttcgcgatc ctgatcgcga ataatgaaaa tttgtgtgcg
     4741 cggcgcaacc tggctgcgga acatgcggat ctcatacagc tcgttattga tcgtcgcctg
     4801 aataatcccc tgatgctgtt ccgccatggt ggtgatgttt tgcagattca aatgcggcag
     4861 caaatgatcg gcaattttgt tacttatgac agtgcggttc gattcctgat cgtgaaccag
     4921 caggccgagc ggcagcagtg agactatctc ttcattgatt gcccgtaaaa tgcgcaattc
     4981 gttattgacc gccgtgttgg gtagactttc tgtactgcga ctggagaaat ggcgaaatgt
     5041 ggtatagcca aataacgcca gcgccagcaa accgatgttc agcagcagtg gcagcagaat
     5101 gttttgcaac gtatccagca ataaggtgcc ataaggaacc tgccagacca ggcgcatatc
     5161 ggtagagttg agtgccgagg agatttcaat cttcgtactg ttaaagtgga tactgacgct
     5221 atccgtccct tctttctcat tatcattgtc tcccgttgcc gtcgcgtctg gctcaaggcg
     5281 gaaactgtcc agcggcatac ccggtgggat caaatcatta atcggcagat caaaagccac
     5341 aaccgttgcc agatgtcccg gctggttgaa cgtggtacgc aaggtaaagt aatgaccgtt
     5401 ctgccaggcc aggcggcgca tgttagaaaa gctttcgcgt tcatcgaggg cgttggcctg
     5461 ttgcaacatc tctgcacgac gtgaatcaac aatgtcgctg acggtcgatt ctttaaatcc
     5521 ggaggtgaga tctttgaggg gcagggttga gatcagcacc agactgttat cctgaccatt
     5581 caggtaatac atcgaccacg gtacattttc tgcgccccac aatgtatcca gataagtgga
     5641 catccgttga gtcatctcaa gcgttgagtt gtcgtgagag ccaaagatta acgcttccgt
     5701 tttgcggcgt ggtttctcca gatagtagac atcctgtttc aggcgcgtct cttgtaaacc
     5761 ttcgccggag gagggggtgg tcgtcgcggc aatgttgtcg tagatctgcc aggtcacgta
     5821 acgccaggta tcgacgcgct tttggatagc gtgggtaatg tcgacaatct ggtaactttt
     5881 atctttcagc caggcgttaa cggcgctttg taccattaca cccatcgtca ccagtaacac
     5941 aatgatcaac agtaaaaaga agcgggtaat gctccccggt aggagtgaaa agcgggtcgt
     6001 ggccgttgtc tctttctgac gcattcgtgt ttatgacctg ttaaaacttc gcgaagtgat
     6061 agggcagtat aaagggtaca gtgtgaaatt ccagactctt accggattga tgattctcta
     6121 ttctcccgtg atatttatca tcctgcattg ggggaaatag atgtacaagc gccattttgc
     6181 tgtacaaata acctacaaaa agcctaacga aattaattgc aaataaacga agattgctgg
     6241 aaattatgct gatgttaatt atttgtgaaa tagttaacaa gcgttatagt ttttctgtgg
     6301 tagcacagaa taatgaaaag tgtgtaaaga agggtaaaaa aaaccggatg cgaggcatcc
     6361 ggttgaaata ggggtaaaca gacattcaga aatgaatgac ggtaataaat aaagttaatg
     6421 atgatagcgg aagatattct agttgcgagt gaaggttttg ttttgacatt cagtgctgtc
     6481 aaataattaa gaataagtta ttgattttaa ccttgaatta atattgcttg atgttaggtg
     6541 cttatttggt cattccgcaa taatcttaaa aagttccctt tcatttacat tctgaaacat
     6601 ctatagcgat aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt ggattattct
     6661 gcatttttgg ggagaatgga cttgccgact gattaatgag ggttaatcag tatgcagtgg
     6721 cataaaaaag caaataaagg catataacag agggttaata acatgaaagt taaagtactg
     6781 tccctcctgg tcccagctct gctggtagca ggcgcagcaa acgctgctga agtttacaac
     6841 aaagacggca acaaattaga tctgtacggt aaagtagacg gcctgcacta tttctctgac
     6901 gacaagtctg tagatggcga ccagacctac atgcgtcttg gcttcaaagg tgaaactcag
     6961 gttactgacc aactgaccgg ttacggccag tgggaatatc agatccaggg caacagcgct
     7021 gaaaacgaaa acaactcctg gacccgtgtg gcattcgcag gtctgaaatt ccaggatgtg
     7081 ggttctttcg actacggtcg taactacggc gttgtttatg acgtaacttc ctggaccgac
     7141 gtcctgccag aattcggtgg cgacacttac ggttctgaca acttcatgca gcagcgtggt
     7201 aacggcttcg cgacctaccg taacaccgac ttcttcggtc tggttgacgg cctgaacttt
     7261 gctgttcagt accagggtaa aaacggtagc gtagatggcg aaggcatgac caacaacggt
     7321 cgtggtgctc tgcgtcagaa cggcgacggc gttggcggtt ctatcactta tgattacgaa
     7381 ggcttcggta tcggtgctgc agtgtccagc tccaaacgta ctgacgctca gaatggtacc
     7441 tatgtaagcg acaataatgt ggtccgtaac tacatcggta ctggtgaccg tgctgaaacc
     7501 tacactggtg gtctgaaata cgacgctaac aacatctacc tggctgctca gtacacccag
     7561 acctacaacg caactcgcgt aggttccctg ggttgggcga acaaagcaca gaacttcgaa
     7621 gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt ccctggctta cctgcagtct
     7681 aaaggtaaaa acctgggtgt catcaatggt cgtaactacg acgacgaaga tatcctgaaa
     7741 tatgttgatg ttggtgcgac ctactacttc aacaaaaaca tgtccaccta tgttgactac
     7801 aaaatcaacc tgctggacga caaccagttc actcgtgacg ctggcatcaa cactgataac
     7861 atcgtagctc tgggtctggt ttaccagttc taatctcgat tgatatcgaa caaagggcct
     7921 gcgggccctt ttttcattgt tttcagcgta caaacacagt tttttggtgt actcttgcga
     7981 ccgttcgcat gaggataatc acgtatggaa ataagcttta cccgcgtggc actgctggct
     8041 gccgcgctct tctttgttgg ttgcgatcaa caaccacaac ccgccaaaac ccacgctact
     8101 gaagttaccg ttcttgaagg caaaaccatg ggtaccttct ggcgtgccag catcccgggc
     8161 attgacgcca aacgcagtgc cgaacttaaa gaaaagattc agacccaact ggacgctgac
     8221 gatcagctgc tttcgaccta taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa
     8281 agtttgtcgc cgtggccggt aagtgaagcg atggccgata tcgtcactac ctcgctgcgc
     8341 attggcgcga agaccgatgg cgcgatggat ataaccgtcg ggccgctggt gaatctgtgg
     8401 ggctttggtc cggaacaaca accggttcaa attccgagcc aggaacagat cgatgcgatg
     8461 aaagccaaaa ccggcttaca gcacctgacg gtcattaatc agtcgcatca gcaatatctg
     8521 caaaaagacc tgccggattt atatgtcgat ctctccaccg tcggcgaagg ttatgcggcg
     8581 gatcacctgg cacgcttgat ggagcaggaa gggatttccc gctatctggt gtcggtgggc
     8641 ggcgcgctga acagccgtgg tatgaacggt gctggcaagc cgtggcgggt agcgattcaa
     8701 aagccaaccg ataaagaaaa cgcggttcag gccgtggtgg atatcaacgg tcatgggatt
     8761 agtacctctg gcagctaccg taactattac gaactggacg gcaaacgtct ttctcatgtt
     8821 atcgatccac aaaccgggcg tcctatcgaa cacaatctgg tatccgtgac ggtgattgcc
     8881 ccgacggctc tggaagccga tgcctgggat actggcttaa tggtactcgg accggagaaa
     8941 gccaaagagg ttgttcgccg ggaaggactg gcggtctaca tgatcaccaa agagggcgat
     9001 aattttaaaa cctggatgtc gccgcagttt aaaagcttcc ttatcagcga aaaaaattaa
     9061 agcgcaagat tgttggtttt tgtgcgatgg tgaccgggca gactaaaggc tatccttaac
     9121 cagggagctg attatgaaat atgccacatg cttaactgac gatcaacgct ggcaatctgt
     9181 cttagcccgc gactcgaatg ccgacggcga attcgttttc gccgtgcgca ccacgggtat
     9241 cttttgccgt ccgtcttgcc gagccagaca tgccttgcgg gaaaatgtct ccttctacgc
     9301 aaatgccagc gaggcgcttg ccgctggctt tcgtccctgc aaacgttgtc agccagacaa
     9361 agccaatccc ctgcaacatc gccttgataa aattacccac gcctgccgac ttctggaaca
     9421 ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg gcgatgagtc catttcattt
     9481 gcatcggttg tttaaagcga ctaccggaat gacgcctaaa gcctggcaac aggcctggcg
     9541 cgctcgccgt ttgcgcgaat tgctggcgaa aggggagagc gtgactacat ctattattaa
     9601 cgccggattc cccgacagca gcagttacta ccgcaaagcc gacgaaacgc tgggcatgac
     9661 ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg
     9721 tgagctgggc cgttgcctgg tggcagaaag tgagcggggg atttgcgcga tattactggg
     9781 cgatgatgac gcgacactaa tcagcgagtt acagcagatg tttcccgctg ccgacagcgc
     9841 gcctgctgat cttacgtttc agcaacatgt gcgtgacgtg atcgccagcc tcaatcaacg
     9901 cgatacgccg ctgacgttac cgctggacat tcgcggcact gcttttcagc aacaagtctg
     9961 gcaggcactg cgcacgatac cttgcggtga aaccgtcagt tatcagcaac tggctaatgc
    10021 catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc tgtgccgcca acaagctggc
    10081 tatcgtaata ccctgtcatc gggtggtccg tggtgatggc acactttccg gttaccgctg
    10141 gggcgtgtcg cgcaaagcgc aactgctgcg ccgcgaagct gaaaatgagg aaaggtaatg
    10201 ttggatctgt ttgccgatgc tgaaccgtgg caagaaccac tggcggctgg agcggtgatt
    10261 ttgcaccgtt ttgcttttaa cgctgcggag caactgatcc gcgatattaa tgacgttgcc
    10321 agccagtcgc cgtttcgcca gatggtcact ccaggcggat ataccatgtc ggtggcgatg
    10381 accaactgtg ggcatctggg ctggacgacc catcggcaag gttatctcta ttcgcccatt
    10441 gatccgcaaa caaataaacc gtggcccgcc atgccgcaga gttttcatca tttatgtcaa
    10501 cgtgcggcta cggcggcggg ctatccagat ttccagccag atgcctgttt gatcaaccgc
    10561 tacgctcctg gcgcgaaact gtcgctgcat caggataaag acgaaccgga tctgcgcgcg
    10621 ccgattgttt ctgtttctct gggcttaccc gcgatttttc aatttggcgg cctgaaacga
    10681 aatgatccgc taaaaagatt gttgctggaa catggcgatg tggtggtatg gggcggtgaa
    10741 tcgcggctgt tttatcacgg tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc
    10801 gactgccgct acaacctgac attccgtcag gcaggtaaaa aagaataaaa ataagaatta
    10861 ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt aattcattgt ccgggttttc
    10921 tgcatggaac ttcttgtact tgtctggcgg cagtatcgct ggccatttat cagtgtgatg
    10981 gcgctaagcc tcgccagtgc ggcattaggc attggcttaa ttgcttttat caatcagcgc
    11041 cttatcgaaa cggcggatac cagtctgctg gtattgccgg agtttctggg gttattgctg
    11101 ctgttgatgg ccgtcactct cggatcgcaa ctggcgctca ccactttggg gcatcacttc
    11161 gtttaccgac tgcgtagtga atttatcaag cggattctcg atactcacgt cgagcgcatt
    11221 gaacaactcg gtagcgcctc gttgctggcg ggattaacca gcgatgtgcg caatatcacc
    11281 attgcttttg tgcgtctgcc ggaactggtg caggggatca ttctcactat cggttcagcg
    11341 gcgtatttgt ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg gatggcagtc
    11401 accatctggg gcggttttgt gctggtggcg cgggtgtaca aacatatggc gaccctgcgt
    11461 gaaaccgaag ataagctgta cacggatttt caaactgtac tcgaagggcg caaagagctg
    11521 actctgaacc gtgaacgcgc cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa
    11581 gagtatcgcc atcatattat tcgcgcagac accttccatc ttagtgccgt gaactggtca
    11641 aacatcatga tgctgggcgc tatcggcctg gtgttctgga tggcgaacag tctcggttgg
    11701 gctgatacca acgttgctgc gacctattcg ttgacgctgt tattcctgcg tacgccgctg
    11761 ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc aggtggcgtt taacaagctg
    11821 aacaaattcg cgctcgcgcc tttcaaagca gagtttccgc gcccgcaggc ttttcccaac
    11881 tggcaaacgc tggagctgcg taacgtgacg tttagctatc aggataacgc gttttccgtt
    11941 gggccaatta acctcaccat caaacgtggc gagctgctgt ttctgattgg tggcaacggt
    12001 agtggaaaat cgacgctggc gatgttgctg acgggcttgt accagccaca aaatggcgaa
    12061 atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg aagattatcg aaaactgttt
    12121 tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc tggggccgga ggggcaaccc
    12181 gctaacccgc aactggttga gaagtggctg gcgcagctga aaatggctca taagcttgag
    12241 ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg
    12301 ctgttgctgg cgctggcaga agaacgcgat attatcctgc tggatgaatg ggcggcggat
    12361 caggatccac acttccgtcg tgagttttat caggtgctgc tgccgctgat gcaggagatg
    12421 ggtaaaacta ttttcgccat cagtcatgat gatcattact ttatccacgc cgaccgcctg
    12481 ctggaaatgc gcaacgggca acttagcgag ctgacaggcg aagagcgcga tgctgcttcg
    12541 cgtgatgccg ttgcccggac ggcataatat tacgacgctg acctggcgga ctgctgccgc
    12601 caggttaata tgctaaaaat cccttcattt ctaataaccc tataattaat caacgaaatt
    12661 ataatgtttc taaaattaga atataattta taaacattat ttaaatgttg ttacttaagt
    12721 gttaccgttg atgccgcgca aatctcacct gcaataaagc gactaaaagt aaggcattaa
    12781 caagatgaaa aaagtgactg ccatgctctt ctcgatggcc gtggggctta atgccgtttc
    12841 gatggcggca aaagcgaaag catccgagga gcaggaaact gatgtactgt tgattggcgg
    12901 cggcattatg agcgccacgt tggggaccta tttacgcgag ctggagcctg aatggtcgat
    12961 gaccatggtg gagcgcctgg agggtgtcgc gcaggagagt tcgaacggct ggaacaacgc
    13021 cggaaccggg cattctgcac tgatggaact caattacact ccgcaaaacg ccgatggcag
    13081 catcagtatt gaaaaagcag tcgccattaa cgaagcattt cagatttcgc gccagttctg
    13141 ggcgcaccag gtcgagcgcg gcgtgctgcg tactccgcgt tcatttatca ataccgttcc
    13201 gcatatgagc tttgtctggg gcgaggataa cgtcaatttc ctgcgcgccc gttacgccgc
    13261 gttgcaacaa agctcgctgt ttcgcggtat gcgttactct gaagatcacg cgcagatcaa
    13321 agagtgggca ccgttagtga tggaagggcg cgatccgcaa cagaaagtgg cagccacgcg
    13381 tacggaaatt ggtactgatg tgaactatgg cgagatcacc cgccagttaa ttgcttcctt
    13441 gcagaagaaa tctaattttt cgctgcaact cagcagcgaa gtccgcgccc taaagcgtaa
    13501 tgacgataac acctggaccg tcactgttgc cgatctgaaa aatggcactg cacagaacat
    13561 ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg ctgaagctgt tacaggaatc
    13621 ggggattccg gaagcgaaag actacgccgg tttcccggtg ggcggacagt tccttgtttc
    13681 ggaaaacccg gacgtggtta atcaccatct ggcgaaggtt tacggtaaag catccgttgg
    13741 cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt ctggacggta aacgcgtcgt
    13801 gctgtttggg ccatttgcca ccttctcaac caaattcctc aaaaacggtt cattgtggga
    13861 tctgatgagt tccaccacca cctctaacgt gatgccgatg atgcacgtcg ggctggataa
    13921 tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg agtgaagagg atcgttttga
    13981 agcgttgaaa gagtactatc cgcaggcgaa aaaagaggac tggcgtttgt ggcaagcggg
    14041 gcagcgcgtg cagattatca agcgtgatgc cgagaaaggt ggcgtactgc gtctgggtac
    14101 cgaagtggtc agcgaccaac aaggaaccat tgccgcgctg ctgggggcat cgccaggggc
    14161 gtcaaccgcc gcgccgatta tgttggatct gctggaaaaa gtatttggcg atcgcgtttc
    14221 cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg tcttatggac gcaagctgaa
    14281 cggtgatgta gcggcaacag aacgcgagtt gcagtacacc agcgaagtgc tggggctgaa
    14341 atacgacaaa ccgcaggcag cagatagtac gccgaaaccg cagttgaaac cgcaacccgt
    14401 tcaaaaagaa gtggcggata ttgcgttgtg atgatgtgcc acatccggca tggtatgccg
    14461 gatgtggcgt atgcagataa gacgcgtcag cgtcgcatca ggcaaccggc tcgggcgtta
    14521 gatgtcggat gcggcgtaac cgccttatcc gacctggcgg ttgcagcact tcacagcagt
    14581 ttagcgaact accgcgttgt caattttctc ttccgccttc cagacgcggt acttcacatc
    14641 tacattgtct ggcgtataca ccacgatcgg cagcttgctg ttataacgca gcattccggc
    14701 atcgcccaga tacgcggtga caaatttctt ctctttcttg ccatccgggc aggccatcat
    14761 cgtcgaaacc ggggaactga ctttatcaaa gacataataa tcatagcccc agccttccag
    14821 cgttttgctt tccagcttcc cgccgagacg atgcaaattg caatcgactt ccagcgtctg
    14881 gccgattaac agttctactt tcagggtaga ttcatcttct tgcggggtta actgaatcac
    14941 ctggcgcttc atgccttttt cagcttgtgg ataaggtgcg attttttcca gtggctggac
    15001 gctttctgcc gcccaggcgg aagtggtggc gaaagcggca aacaatactg caggtagaat
    15061 ggtcttcatt tttttgcttt ccttcttcat ttataggttt tcaccgatga tgtcgctgaa
    15121 ggttaacata ttcatcagat ttattaattt gctttatgaa taacttaaag aacattcaga
    15181 taattttacc tgctctttta gtgttaaatt ctgatgagaa aacaaaccag cagaaatatc
    15241 tctggcgaat taatcgctat ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc
    15301 cgtttttgcc acgcttcctg ccactctttt gatcctgcta caggttttac cccgatcggg
    15361 gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta
    15421 tgcggtttat ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga
    15481 agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta
    15541 tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcact cgctgcgacg
    15601 cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggtggc tatccgagcg
    15661 ttaatttcaa aaataacgag tgcagcttct gttacgcctg cgcgcaggct tgccccgaat
    15721 cgctattttc tccgcgccac accagggcct gggatttgct gttcaccatc ggggaggcgt
    15781 gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa
    15841 ttatctttcg cccgacgctc tccgggatct accagccgca acttaatagc caactttgca
    15901 acgcatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc
    15961 atgtacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca
    16021 gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg
    16081 agcggccagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt
    16141 gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa
    16201 gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg
    16261 ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg
    16321 gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt acgggttgcg
    16381 gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac
    16441 cggttaaccg tggtctgaac tgcattaagg gctatttcct gcccaaaatc atgtacggta
    16501 aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg
    16561 aatttacccc aatcacctgg gatcaagcct tcgatgtgat ggaagagaaa ttcaaaaccg
    16621 ccctgaaaga aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct
    16681 gggaaggtta tgccgcgtcc aagctgttta aagcgggatt ccgttcgaac aacatcgacc
    16741 cgaacgcgcg tcactgtatg gcgtcggcgg tagttggctt tatgcgtacc tttggtatgg
    16801 atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggct
    16861 ccaacatggc ggagatgcac ccgatcctct ggtcgcgcat cactaaccgt cgtctctcca
    16921 accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg
    16981 ataacggcat catctttacg ccgcaatctg acctggtgat cctgaactat atcgccaact
    17041 atatcattca aaacaatgcg ataaatcagg acttcttcag caaacacgtt aacctgcgca
    17101 aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga
    17161 agaatccggg ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg
    17221 ctgaatatac gctggaaaaa accgccgaaa tgaccggcgt gccgaaagac cagttagaac
    17281 aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct
    17341 tcaaccagca tacccgtggt gtgtgggcca acaacctggt ctacaacctg cacctgctga
    17401 ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcag ccttccgcgt
    17461 gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga
    17521 ctaacgagaa acatcgtgat atttgcgaga agaagtggaa tatcccgagt ggcaccattc
    17581 cggcgaaaat cggtctgcac gcggtggcac aagaccgtgc gctgaaagac ggcaagctga
    17641 atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatt aatgaagagc
    17701 gtatgccagg ctggcgcgat ccgcgcaact ttatcatcgt ttccgatccg tatccgacag
    17761 tcagtgcgct ggcagcggac ttgatcctgc cgaccgcaat gtgggtagag aaagaggggg
    17821 cttacggtaa cgccgaacgc cgtactcaat tctggcgtca gcaggtacag gcaccgggcg
    17881 aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag
    17941 tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag
    18001 ttctgtatgc caccccggaa gtgagcaaat tcccgctatc cgaactggcg gaagatcagc
    18061 tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg
    18121 catggtttgg tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac cacaaagcac
    18181 gcggtctgcg ctggccggtg gtgaacggta aagaaacgca gtggcgttac agcgaaggta
    18241 acgacccgta cgtgaaagcg ggcgaaggct acaagttcta cggtaaaccg gatggcaaag
    18301 cggtgatctt cgcgctgccg ttcgaacctg cggcggaagc accggatgaa gagtacgacc
    18361 tgtggctctc taccggacgc gtcctggagc actggcatac cggcagtatg actcgccgtg
    18421 taccggaact gcaccgcgcc ttcccggaag cggtcctgtt tattcacccg ctggatgcga
    18481 aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga
    18541 tctcgattgt cgaaacgcgc ggtcgtaacc gtccgccgca gggcctggtg tacatgccgt
    18601 tcttcgatgc cgcacagctg gttaacaaac tgacgctgga tgcgaccgat ccgctctcga
    18661 aagagacgga cttcaagaag tgcgcggtca aactggagaa ggtgtaagcc attatgtccc
    18721 ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt cgcacagctg
    18781 gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg
    18841 gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc
    18901 gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggcct
    18961 ctggcctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg
    19021 aggacattcc gtgcgccaaa gtgtgcccga gcggtgcgct ggatcgtgag atcgaatcga
    19081 tcgacgacgc gcgaatgggg ctggcggtac tggtggacca ggaaaactgt ctcaactttc
    19141 aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc
    19201 tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca
    19261 gcgacgcctg taccggttgc ggtaagtgcg aaaaagtgtg cgtgctggaa caaccggcaa
    19321 tcaaggtgtt accgctgtca ctagcgaaag gggagttagg tcaccattac cgcttcggct
    19381 ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggagaaa
    19441 aaaggctggt ggcgcagtca ccgttggtta gtgttacgtc gcctttgtca gttcttcgtg
    19501 ctggggatgt ttttgagcgg tccgtggttt ggtgtgtgga tcttgcacgg caactacagc
    19561 agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt
    19621 gccagcggtc atctgccggc ctcggtggcg ctgaccggag cggtgattat caccgtgctc
    19681 tacgccctgg caggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc
    19741 gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac gatcccacgc
    19801 cacattcgct acgtgctgtt ggtggtcatt ctgctgggat cggccttaac cggcacgctc
    19861 atctgggaat ggatcaaccc cgtttccctg atggggcgca gtctggtaat gggattcggc
    19921 agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc
    19981 tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg
    20041 attaccgttg cggccaccga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt
    20101 tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc
    20161 accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt
    20221 acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag
    20281 cgctgtgtca atggacggcg atgctggccc tggtggtgag cggcgcggtt tgggcggcta
    20341 atggcgtcga ttttagccag tcgccggaag tatccgggac gcaggaaggg gccattcgga
    20401 tgccaaaaga gcaggatcgg atgccgctga actatgtgaa tcagccgcca atgatcccgc
    20461 atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg
    20521 tcgaaagcta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg
    20581 acggcaaagt gggcgcagaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac
    20641 cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga
    20701 aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga
    20761 aatggtggcg tacccccagc cgtctggcgc tggggacgct gctgttgatc ggttttgttg
    20821 gcggcatcgt cttctggggc ggctttaaca ccgggatgga aaaagccaat accgaagagt
    20881 tctgcattag ctgccacgaa atgcgtaaca cggtgtatca ggaatacatg gattccgtgc
    20941 actacaacaa ccgtagcggc gttcgtgcga cctgtccgga ttgtcacgtt ccgcacgagt
    21001 ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg
    21061 gcgttattga cacgccgcag aaatttgaag ctcatcgcct gacgatggca cagaatgagt
    21121 ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata
    21181 tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg
    21241 ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgcgaag
    21301 tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt
    21361 gcgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt
    21421 gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg
    21481 ggttgtctcg ccctgatgcg ggtgacgttc tctggcaagg gcagcccttg catcaggtac
    21541 gcgacagcta ccatcaaaac ctgttatgga tagggcatca gccggggatc aaaacccggc
    21601 tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg
    21661 aagccctggc gcaggccggg cttgccggat tcgaagatat tccggtaaat caactttctg
    21721 ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga
    21781 tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta
    21841 tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg
    21901 ttgctgaaaa taaaattcgc cgcatttcac tgacgcaaac gaggggcgaa taatgttctg
    21961 gcgcattttc cgtcttgagc tgcgcgtagc gtttcgccat agcgccgaaa tcgccaaccc
    22021 gctgtggttc ttcctgattg tcattaccct ttttccgctc agtatcggtc cggagccgca
    22081 actgctggcg cgtattgcgc cgggcattat ctgggttgct gcgctgcttt catccttgct
    22141 ggcactggag cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt
    22201 gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac
    22261 cggtctgccg ttactcatcc tttcgcccct ggtagcaatg ctactgggaa tggatgttta
    22321 tggctggcaa gtgatggcgt taacgctact gctgggaacc cctacgcttg gctttcttgg
    22381 tgcgccgggc gtggcgctga cagtgggact taagcgcggt ggtgtgctgc tcagcatact
    22441 ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg acgcggcttc
    22501 tatgcatttg cccgttgatg ggtatctggc aattttaggc gcgttgctgg caggcaccgc
    22561 gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat
    22621 tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg
    22681 atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt
    22741 gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggtt ttgctccggc tgattatcag
    22801 cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc
    22861 atttatgcat caatggcggt ggcggcgttt attggccttg tctggcagat gaaaatggcc
    22921 aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt
    22981 accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgcctg
    23041 acttctgaac tggtactgct gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc
    23101 gacgaccgcc gtctggcggg ccgtgcggca ggtattctgg tgctgattgg cgtggtgaat
    23161 ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg
    23221 atgcaacaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc
    23281 ctgctgctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa
    23341 aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat
    23401 ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg
    23461 tggtgatgac cgttattccg ctggtgattt tggtcgtgca ctcggtgatg cagcatcgcg
    23521 caattctgcg tggcgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac
    23581 agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgct
    23641 ggcggggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct
    23701 cttttatacg ccgggggaga ttctctacgg caagcgtgaa actcagcaga tgccggaagt
    23761 cggtcagcgt ctgcgcgttg gcgggatggt gatgccgggg agtgtgcagc gcgatcccaa
    23821 ttcgctaaaa gtaactttca ccatttacga cgctgaaggt tcagtagatg tctcttacga
    23881 aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga
    23941 aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgatgaaa actacacgcc
    24001 gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga
    24061 cccagcatca tgatgccaga aattggtaac gggctgctgt gtctggcgct aggaattgcg
    24121 ctgctgctgt ctgtgtatcc gctatggggc gtggcgcgcg gggacgcgcg catgatggcg
    24181 tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg
    24241 gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa ctccaatacc
    24301 cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctcctg
    24361 ctgtgggtgc tactgatgag cggctggacc tttgcggtag cgatttttag tcagcgtatt
    24421 ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagtgt cggctttttg
    24481 ctgttcattc tctttacctc taacccgttc tcacgcacgc tgccgaattt cccgattgaa
    24541 gggcgcgatc ttaacccgct gttgcaggat ccggggctga tcttccatcc gcctctgctc
    24601 tatatggggt acgtcggttt ctcggtggcg tttgctttcg ccattgcttc tttgttgagt
    24661 gggcgtctgg acagcaccta tgcgcgtttt acccgcccgt ggacgctggc ggcgtggatc
    24721 ttcttgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggc
    24781 ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact
    24841 gcgctgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta
    24901 ctgctggcaa tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt gcgttccggc
    24961 gtgctggtat cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt tatcctcgcc
    25021 tttatggtgt tggtgattgg cggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt
    25081 cgctcacgcg taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt
    25141 ttgctggtcg ccgcgatgct ggtggtatta ctggggacgc tgctgccgct ggtgcacaag
    25201 caactgggac tgggcagtat ttcgattggt gaaccgttct tcaacaccat gtttacctgg
    25261 ctgatggtgc cgtttgcgct gctgcttggt gtcggtcctc tggtgcgctg ggggcgcgat
    25321 cgcccacgta aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtcg
    25381 ctgctgctgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct cggtctggca
    25441 atggcctgct ggattgcgat gctggcaatt gcggaagctg cgctgcgtat ttcacgtggc
    25501 acgaaaacca ccttcagtta ttggggaatg gtggcggctc acctggggct ggcagtgaca
    25561 attgttggta ttgcctttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc
    25621 ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact
    25681 ggtccgaact ggcgtggcgg tgtggcgact atcggcgtaa cgcgaaatgg caagccggaa
    25741 acagtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg
    25801 gcaattgacg gcggcatcac gcgtgacctg tacgcggccc tcggtgaaga gctggaaaac
    25861 ggcgcgtggg ccgtgcgtct ttactacaaa ccgtttgttc gctggatttg ggcgggc
//
LOCUS       DAZGTJ010000077        25839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000077 DAZGTJ010000000
VERSION     DAZGTJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.077"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(56..751)
                     /gene="aqpZ"
                     /locus_tag="ACHK8E_003678"
     CDS             complement(56..751)
                     /gene="aqpZ"
                     /locus_tag="ACHK8E_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP0345893.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1177..2835
                     /gene="ybjD"
                     /locus_tag="ACHK8E_003679"
     CDS             1177..2835
                     /gene="ybjD"
                     /locus_tag="ACHK8E_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP0345894.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2832..3788)
                     /gene="ybjX"
                     /locus_tag="ACHK8E_003680"
     CDS             complement(2832..3788)
                     /gene="ybjX"
                     /locus_tag="ACHK8E_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP0345895.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3939..5054
                     /gene="macA"
                     /locus_tag="ACHK8E_003681"
     CDS             3939..5054
                     /gene="macA"
                     /locus_tag="ACHK8E_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP0345896.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5051..6997
                     /gene="macB"
                     /locus_tag="ACHK8E_003682"
     CDS             5051..6997
                     /gene="macB"
                     /locus_tag="ACHK8E_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP0345897.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEVRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7070..7294)
                     /gene="cspD"
                     /locus_tag="ACHK8E_003683"
     CDS             complement(7070..7294)
                     /gene="cspD"
                     /locus_tag="ACHK8E_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP0345898.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7444..7518
                     /gene="yljB"
                     /locus_tag="ACHK8E_003684"
     CDS             7444..7518
                     /gene="yljB"
                     /locus_tag="ACHK8E_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP0345899.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7617..7937
                     /gene="clpS"
                     /locus_tag="ACHK8E_003685"
     CDS             7617..7937
                     /gene="clpS"
                     /locus_tag="ACHK8E_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP0345900.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7968..10244
                     /gene="clpA"
                     /locus_tag="ACHK8E_003686"
     CDS             7968..10244
                     /gene="clpA"
                     /locus_tag="ACHK8E_003686"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP0345901.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10766..10853)
                     /locus_tag="ACHK8E_003687"
     tRNA            complement(10766..10853)
                     /locus_tag="ACHK8E_003687"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10817..10819),aa:Ser,seq:gga)
     gene            complement(11107..11325)
                     /gene="infA"
                     /locus_tag="ACHK8E_003688"
     CDS             complement(11107..11325)
                     /gene="infA"
                     /locus_tag="ACHK8E_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP0345902.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11610..12314)
                     /gene="aat"
                     /locus_tag="ACHK8E_003689"
     CDS             complement(11610..12314)
                     /gene="aat"
                     /locus_tag="ACHK8E_003689"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP0345903.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12356..14077)
                     /gene="cydC"
                     /locus_tag="ACHK8E_003690"
     CDS             complement(12356..14077)
                     /gene="cydC"
                     /locus_tag="ACHK8E_003690"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP0345904.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAALEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14078..15844)
                     /gene="cydD"
                     /locus_tag="ACHK8E_003691"
     CDS             complement(14078..15844)
                     /gene="cydD"
                     /locus_tag="ACHK8E_003691"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP0345905.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15967..16932)
                     /gene="trxB"
                     /locus_tag="ACHK8E_003692"
     CDS             complement(15967..16932)
                     /gene="trxB"
                     /locus_tag="ACHK8E_003692"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP0345906.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17476..17970
                     /gene="lrp"
                     /locus_tag="ACHK8E_003693"
     CDS             17476..17970
                     /gene="lrp"
                     /locus_tag="ACHK8E_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP0345907.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18105..22172
                     /gene="ftsK"
                     /locus_tag="ACHK8E_003694"
     CDS             18105..22172
                     /gene="ftsK"
                     /locus_tag="ACHK8E_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP0345908.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22331..22942
                     /gene="lolA"
                     /locus_tag="ACHK8E_003695"
     CDS             22331..22942
                     /gene="lolA"
                     /locus_tag="ACHK8E_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP0345909.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22953..24296
                     /gene="rarA"
                     /locus_tag="ACHK8E_003696"
                     /gene_synonym="mgsA"
     CDS             22953..24296
                     /gene="rarA"
                     /locus_tag="ACHK8E_003696"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP0345910.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24387..25679
                     /gene="serS"
                     /locus_tag="ACHK8E_003697"
     CDS             24387..25679
                     /gene="serS"
                     /locus_tag="ACHK8E_003697"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP0345911.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         6481 a         6395 c         6940 g         6023 t
ORIGIN      
        1 gcggtttgta acgtcgaaga taaaacaacc agggcagata aaatgccaat gctaattaat
       61 tacgcttttc cagcagggtc cggtaaatca gaccaccgat aataccgccg acaattggca
      121 ccacccagaa gaaccacagt tgttctaacg cccagccgcc ctggaagata gcagccgcgg
      181 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actgattaag tgaatcaggg
      241 ttaaggccag accaatagcg atcggcgcaa aaccagcagg agcgaatttg tcggttgcgc
      301 cgtggatcac cagcaggaaa cctgcactca ataccagttc aactaccagc gcggaaagca
      361 tggaataacc gcctggcgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg
      421 cgtcaaaacc tgttttacca ctggcaatta aatacagcag cgccgccgca acaataccgc
      481 cgacaacctg ggcaattacg tagccaacga cttcttttgc cgggaaacgt ccgccagccc
      541 ataaaccaat agtgaccgcc gggttaaaat gaccaccaga aatatgacca acagcaaagg
      601 ccatcgtcag aacggtcaga ccgaatgcca gcgccacgcc ggcaaaacca atgcctaatt
      661 ccgggaagcc tgcggccagt acggcactac cacagccacc aaaaacaagc cagaaagtac
      721 caaaacattc agctgctaat tttctgaaca tatccacctc aattaaaaat tgaccctgtg
      781 aaaaatatgg tcgttttata gggtcgtcgt aaaaaagtga cgacggaaat aatgcgcggc
      841 tattttaaaa acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttaa
      901 ggcaacagcg ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcgaaa
      961 gatttaatcc tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg
     1021 tttttgcaga cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca
     1081 tttgccatgc ttaaatgtga tgtcatcacg tattagcatg gcctttcccg ttatactgcc
     1141 agcgtaaagg ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt
     1201 gtgggttttc gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt
     1261 ggggagaacg cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca
     1321 gaatcagatc tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac
     1381 gggcgagaac atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat
     1441 cgggttcgcc gctatcggcc gctggaagcg tgctggacgc catgcaccga tggttatcac
     1501 cgtatttttt atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc
     1561 agttttctcg ataaagacgg acatccgatt gatgttgagg atattaacga tcaggctcgt
     1621 catctggtgc gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt
     1681 cgtaacggca cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc
     1741 ctcgcccgtg aattatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga
     1801 ctttccgcaa tggtgcaact tcttgagcat tatttttctg agcagggagc tggacaggcg
     1861 cgatatcgtt taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat
     1921 atcatcaacc ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc
     1981 ctgtttgcta ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca
     2041 ttgttgctga tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg
     2101 catctgttga atcttctgcc cttgcagcgc attgccacca ccaactcggg tgagttactt
     2161 tcgttaacgc cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc
     2221 tggcgtctgg ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt
     2281 cgttttaacc gtccgtcatc gctgtttgct cgctgctggc tgctggtgga aggggaaacg
     2341 gaaacctggg ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaagga
     2401 atcaaggtca ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga
     2461 atggggattg aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct
     2521 acggtacgca gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta
     2581 ccggcgctgg atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc
     2641 gtggcgcaaa tcccggaaaa tgtaccgatg aatctgcgca aaattatctc gaaagcgatc
     2701 catcgctctt ccaaacccga tcttgccatt gaagtggcaa tggaggccgg acgtcgtggt
     2761 gtggactccg taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc
     2821 ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc
     2881 tcatagcgcc gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt
     2941 gctatctgtg ccggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc
     3001 cagaacgcgt tgtaatcggc atggatcttg ccttctttat cacgataacg caggctgcgg
     3061 taaatatgcg tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac
     3121 agacaggccg cttccatcac aaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca
     3181 ttctggattt cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatt
     3241 gttcttttcc ccttatattc acacagggta aacgtgatct ctgccagagg aataccttcg
     3301 ctgttgcgga acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc
     3361 tcaagcgtga actgctcgcc gtttttgcct tccagtttag ccagttgcag cccgggggta
     3421 ttcaaatata agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa
     3481 cgtaacgcct ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga
     3541 cgcacaggca gtcgcggctg gcgcgttaac aacacattga gattgggcca gtgggaaagt
     3601 tcgttcatcc actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg
     3661 cgaaaactac ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga
     3721 aaaagtgaca ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac
     3781 atattcatga aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa
     3841 acatcaatag tgtgattact aatgtaaatt ttagggtttt gttgatattt cgttgaagtt
     3901 aatgacccgg attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga
     3961 agaagcgtta cgttattgcg ctggtgataa tcatcgtcgg gctgattacg ttatggagaa
     4021 ttcttaacgc acccgtgccg acttatcaga cgctgattgt gcgccccggt gatttacagc
     4081 aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa ggttgatgtg ggcgcgcagg
     4141 tcagcggtca gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc
     4201 ttttaggggt tattgatcct gaacaggctg aaaaccagat taaggaggtg gaagcaacgc
     4261 tgatggagct acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga
     4321 cgtattcccg tcagcaacgt cttgcacaaa cgcaagcggt ttcacagcag gatctcgata
     4381 acgccgcgac ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca
     4441 agcgcaatca ggcctctctc gatacggcta aaaccaatct cgattacacc cgcatcgttg
     4501 ccccgatggc cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg
     4561 cacaacaagc accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc
     4621 aggtttctga agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc
     4681 ttggcgatcc actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa
     4741 agattaacga cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttac
     4801 tgcggctgga tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga
     4861 cgatccctct gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt
     4921 tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg
     4981 ttgagattgt caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag
     5041 gagctgcgca atgacaccct tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg
     5101 tgatgagcag gttgaggtgc tgaagggcat cagcctcgat atttatgctg gtgagatggt
     5161 ggcgattgtc ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct
     5221 ggataaggcc accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc
     5281 cgatgcgctg gcgcaactac gccgcgagca tttcggcttt attttccagc gttaccattt
     5341 gctttcgcat ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga
     5401 gcggaaacag cggctgcttc gcgcccagga gttgctgcaa cgactggggc tggaagaccg
     5461 tacagagtat tacccggcac agctttcggg tggtcagcaa cagcgcgtca gcatagcgcg
     5521 ggcattgatg aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag
     5581 ccattctggc gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt
     5641 gattatcgtt acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaagttcg
     5701 cgacggtgaa attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac
     5761 ggaacctgtt gtcaacacgg cgtctggctg gcggcagttt gtcagcggtt ttaacgaggc
     5821 gctgacgatg gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct
     5881 ggggattatt atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa
     5941 acaaatggtg ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg
     6001 gaaagatttt ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat
     6061 cgccatccaa aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg
     6121 cctgcgttat aacaatgttg atgtggctgc cagtgccaat ggcgtgagcg gcgattattt
     6181 taatgtctat ggcatgacct tcagtgaagg gaacaccttt aatcaggagc agctgaacgg
     6241 tcgtgcgcag gtggtggtgc tcgacagtaa tactcgccgc cagcttttcc cccataaagc
     6301 agatgtggtt ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc
     6361 ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag
     6421 cacgatgtcc gggcgagtta tgggccagtc gtggctgaac tccattactg tcagggtgaa
     6481 agaaggcttt gacagcgctg aggcggaaca gcaactcacg cgtttacttt cactgcgcca
     6541 cggtaagaag gatttcttta cctggaacat ggacggcgtc ttgaaaaccg ttgaaaagac
     6601 cacacgtact ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg
     6661 tattggtgta atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat
     6721 tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga ttgaagccgt
     6781 actggtttgc ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac
     6841 cttacagctt ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc
     6901 gtttctctgc tcgacggtca ccgggatttt atttggctgg ttacccgcac gaaatgcggc
     6961 acgactggat ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc
     7021 gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg
     7081 ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga
     7141 catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg
     7201 aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg
     7261 cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat
     7321 acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct
     7381 agaaatacaa tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca
     7441 ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct
     7501 gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc
     7561 tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg
     7621 gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc
     7681 taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattatact ccgatggagt
     7741 ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga
     7801 tgctcgctgt tcactaccag gggaaggcca tttgtggagt ctttaccgct gaggttgcag
     7861 aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta
     7921 cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag
     7981 aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta
     8041 tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg
     8101 aagcgtgctc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa
     8161 ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc
     8221 agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg
     8281 gtgcaaacgt tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc
     8341 gcaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag
     8401 acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg
     8461 gggaggaacg tatggagaat ttcacgacga acctgaatca gcttgcgcga gtgggcggaa
     8521 tcgacccact gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc
     8581 gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg
     8641 aggggcttgc atggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga
     8701 tttactctct cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa
     8761 aacgttttaa agcgttgctc aagcagctgg agcaagacac taacagcatc ctgtttattg
     8821 atgagatcca caccattatc ggtgcgggtg cagcgtctgg tggccaggtc gatgcagcta
     8881 acctgatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc
     8941 aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg
     9001 atattactga accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt
     9061 atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcagcg gtagagctgg
     9121 cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgacgtt atcgacgaag
     9181 cgggcgctcg cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg
     9241 atattgagtc cgtggtggcc cgtattgcgc gcattccaga gaagagtgtt tcgcagagtg
     9301 accgcgatac cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata
     9361 aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac
     9421 ataaaccggt cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga
     9481 cggtacagct ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata
     9541 tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc
     9601 agggcggttt gctgactgat gcggtcatca agcatccaca tgcggtgctg ctgctggacg
     9661 aaatcgagaa agcgcacccg gacgtgttca atattctgtt gcaggtgatg gacaacggta
     9721 cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtattg gtgatgacca
     9781 ccaacgctgg ggtacgagaa actgagcgta aatcgattgg tcttatccac caggacaaca
     9841 gtaccgatgc gatggaagag atcaagaaga tctttacgcc ggagtttcgt aaccgtctcg
     9901 acaacattat ctggttcgat catctctcca ccgacgtgat ccatcaggta gtggataaat
     9961 tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg
    10021 aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg
    10081 cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgttg tttggttcgc
    10141 tggtggacgg cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttatg
    10201 gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag
    10261 gttggtgaag tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaggag
    10321 gttctgaccc tttttttgat gatgatggta atttgttgat aattagtgct gcgggaaggt
    10381 aaggataaaa aagggtggta gcaggaaaat gagatggttt tgctttatta acaacgggct
    10441 agacgtgtag tatttgagtt cactgccgta caggcagctt agaaagccaa ccgaaagaaa
    10501 aactcgattt ttataccgtt cactgccgta cagacagata aaatgcgaaa aaaaaacgta
    10561 ctttcgtacg agctcttctt tgaatatggc ggtgaggggg ggattgactc gcttcgctcg
    10621 ccctgcgggc agcccactca ctgtgttcgt ggtctgtcca actggctgcg ccagttgtcg
    10681 aaccccggtc ggggcttctc atcccccctg gagtgcaata tgcgaaaaaa aagctcgcac
    10741 tttcgtacga gctcttcttt gaatatggcg gtgagggggg gattcgaacc cccgatacgt
    10801 tgccgtatac acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt
    10861 tttgctacca aacctcatgg gtggcaacgg ggcgctacta tagggagttg gagtaaaacg
    10921 gtcaagaaga attttaatga taattattgt ttgctcatac tgtaaacaag ttgtgcagta
    10981 tatctacatc gagacaagtt atggacttat acttccaaag tacttcatac atatcacaaa
    11041 ataaaaaggc cggctaaacc gaccttttac tcgttctttc tcttcgccca tcaggcggta
    11101 aaacaatcag cgactacgga agacaatgcg gcctttgctc aggtcgtacg gggtcagttc
    11161 aacagtcact ttgtcgcccg tcaggatgcg gatgtagttt ttgcgcattt taccggagat
    11221 gtgtgcagta accacgtgac cgttttctaa ctctacgcgg aacatggtat taggcaacgt
    11281 ttcaagaacg gtaccttgca tttcaatatt gtcttctttg gccatctaat cctctggggt
    11341 atcactaccg taatttgaac cggcaagata atgccgaagt tctgtaaata agtaaagatt
    11401 tgcgcgctaa atcgcaacaa acaggttcgg cacattactc cgaaaacaca cggctaagcc
    11461 gcaccaaaag cgcaacgtat aagggagcgg tgagataaac gatgggcgtt acctgacgcg
    11521 aaaaattcct tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa
    11581 cactctgggg agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa gcaccgtggt
    11641 acccagaaat tattcggcaa tcgtccgagg cgcatttgat tgagataatt gaggtaatcc
    11701 cggcggggaa tttcgcaggc accaagcgac gctgtgtgat cgttaaggac ctggcagtcg
    11761 ataagtttac cgccatgacc aataaattcc tcacagaata ccagaagcgc cgttttagac
    11821 gcattttcca tccggctgaa catggactcg ccacaaaata gcgttccctg ggccacgccg
    11881 tacataccgc cgacaagctc atcttcacgc cagacttcaa tagagtgggc atgaccgagt
    11941 tcgtgaaggc gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga
    12001 tcgctggcac agccttcaat gacctgacca aaagcgtaat tcatcgtgac tcgatagggc
    12061 gagcgtttat gaaatcgctt catactacgg ctgatatgca gtgattcagg ccaaagcacc
    12121 gcgcggggat cgggcgacca ccagaggatg gggtcacctg gagaaaacca cggaaaaata
    12181 ccacgctggt aagccattaa caggcgcgca gggctaagat cgcctcccag tgccagcagg
    12241 ccgttaggct cacgtaatgc gccttccggg gaagggaagg ctattgaatg gcgagaaagc
    12301 tgaaccaggc gcatgaccgc aaaactccac gcaagtcgga tcgttcaata atagcttaca
    12361 aaccctgctt aaactggtaa taacgcccct gtctggcaag cagttctgcg tgagtacctt
    12421 gctcaataat ttgcccgttg tccatcacta ttatttgttg gaaacgagag agtccgcgaa
    12481 gtcgatgggt gaccattaac accgttttct catgcatcat ttctgcaagc aattcaagga
    12541 tctgactttc ggttgtggca tctaagcctt ctgtaggttc atccagcaat accagcggcg
    12601 cattatgtaa cagcgcacgg gcgatagcca gacggcgtag ttcaccaccg gagagctggc
    12661 gtccgccttc acccaaccaa ctgttgagac ctgcatcctc gagcagcttc tccagaccaa
    12721 cgcgacgtaa gatctccgcc agagcctcat cactactgcc aggcgaggcg agtaacagat
    12781 tatcacgcag cgtggcgcta aacagatgca ctcgctgagg aacaacgctg atggtctgtc
    12841 gtagagtcgc ttcattcagg ctggctatgg ggctaccgtt aagcaaaatc tcgccctgtt
    12901 gcgggtccca tgcgcgggtc agcagttgta acagtgttga tttgccgcat ccggttcgcc
    12961 cgagaatcgc tatatgttcc ccggcgttta cctgaagaga aatcccttta agtgcctgtt
    13021 gggattgctc cggataattg aattgaacat cccgtaacgt cagcgaaacg cgatcggcaa
    13081 cacgagtttg ggtatcaggg aaggtgactt ccggtttttg atccgttaag tcagtgatcc
    13141 gtacggcaga ggcaatgact tgccccagat gctgaaatgc acccgttact ggtgccagtg
    13201 cttcaagcgc ggctaacgcg cagaagacaa acagggcaat taacgcgccg ggttgagcat
    13261 tgccgccaac gccgccagac gccatccaca gcatcaggat caccgctaac gcgccaatga
    13321 gcagcattat cgcttgcgac aatgcggtga gttcagattg acgacgttgc gcttccagcc
    13381 actgaatttc tgtattctct aattgcgtgc gataacgatc gctggcacca aagatggtca
    13441 gctcagcttg cccttgcagc caggccgtca gttgttggcg atactgcccg cgaagatgag
    13501 tcagattttg cccggtgctt tttcccgcac gataaaacag tggtggcata aggaaaagcg
    13561 tcagtaacat aatgccgccc agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga
    13621 ttgtcaccac cataatcacc acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa
    13681 gatgatcgag cgtatcaaca tccgccacca cgcggttgag caattcgccc tgacgatagc
    13741 gcgccagtcc ggcaggggat aggggcagca atttgctgaa ggtgtaaatg cgcagatgct
    13801 gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc aaaatagcgc ccggcagtac
    13861 gggtgattgc tgcgccacgc acgcccgcag cgggtagcat atagttgaag ctgtacagtc
    13921 cggcaacccc cgctaccgct gaggcggaga ggaaccagcc ggaaagtgtc aacagaccga
    13981 tactggcgag cagcgtgaca attgccagca caataccaag acttaacatc catttatgac
    14041 gtttatacag tgccagatag ggtagcaaag cgcgcattta aatctcctcc tgacgatggg
    14101 ccagcaatgt ggcgaaagga ccaccagcca cgcttaattc cgcgtaacgt ccttgctcaa
    14161 taatctgacc atcctgcatt acccaaatga catcccagtc agcaagatct tctaactggt
    14221 gggtgaccat taacgttgtc tggcgcagag aggcggcatt aagcgcctcc attacgcgct
    14281 gttcactgtg agcatcaagg ctggcagcgg gttcatccaa cagtaatagc gaacagggat
    14341 ttagtaatgc tcgggccacc gccacgcgct gcgcctgccc cacggaaagg cgggcagcct
    14401 ggtcgccaac aggcgtatca acgccttgtg gcaggagcgg tagaaactcg ctgacccagg
    14461 cgttatccag cgctgtttgt aactcgtgtt cgctggcatc aggtcgtgcc agtagtacgt
    14521 tatcccgtag tgttgctgcc ggtaattgtg ggttttgccc aacccaggag agatgcttac
    14581 gccatgattc cggtgataaa tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat
    14641 atgagagaaa accagaaagc gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa
    14701 ccaacactgc acgttggcct gctggcaaag taaagttcag cggtccagcc agcgttttac
    14761 cttctggtga tgtaataaac agatcctcgg cttcaatggt caccggatcg gttgatgcta
    14821 attccgcctc accacgttgc ggatgggcta gcggggtttc cataaacgtt ttcagactgt
    14881 cagctgcgcc aacagcctgg gctttagcat gataaaacgt accgagatcg cgtaatggct
    14941 ggaaaaactc cggcgcaagg atcagggcca gaaaacccgc agccagcgtc acgccagtat
    15001 catagtgacc aaaatccagc tcgccgagat aggaaaaacc aaagtagacc gccaccagag
    15061 caattgacag cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc agccgtagca
    15121 cttccattgt ccgttggcgg aaatcttccg aagcagaacg aatactttca atttcagctt
    15181 caccacgacc aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac
    15241 ttaagcgagc aagagcgaga aagttacgtc ggttagcatc ggcagccccc attccaacca
    15301 gcgccataaa caacggaatt aacggtgcag tgcccagcag aataagcgcc gcagcccagt
    15361 tagacgggaa gatcgccacc acaatcagca aaggcacgga cactgccagc gccatttgcg
    15421 gcaggtagcg tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc
    15481 agctccccgc cggtttaccc tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa
    15541 cctgacggcg gatggcaaag cggatatgct gcccggcgtg ataacccacc cgttcgcgta
    15601 accagaccac ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag ggaagcagca
    15661 gggcttcacg gggaatattc tccataatca tatgttgcag aattcgcgcc atgaaccagg
    15721 cctgggcaat gatcaatatg ccgctcacaa agcccagcag acgagaaata ttcagccaac
    15781 gttgggagat gacgctttgc tgttttaacc agcgggttaa ctctttttga cgggatttat
    15841 tcattgcacg cttagcaggt gagttatcag aattatttgc agagcaatgt tacaacgagg
    15901 aaaaaataaa ggcgacccat agtcgcatgg tgtcgccttc tttacttttg ttactgattt
    15961 gtaaaattat tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat
    16021 gcagcctgta ccagcagatg taatagcctg gcgataaatg tgatccatca cgtcgcctgc
    16081 ggcaaagacg ccagggatgc tggtctgggt ggcattacca tgaatacccg actgtacttt
    16141 gatgtagccg ttttccagtt ccagctgccc ttcgaaaata gccgtattcg ggctgtgacc
    16201 gatagcaaca aacagaccgg caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc
    16261 gcgcagacga acgccagtga cgcccatctg atcgccggtc acctcttcca gcgtacggtt
    16321 ggtgtgtaga atgatgttgc cgttctccac tttatccatc agacgcttaa taaggatttt
    16381 ttccgcgcgg aaaccgtcac ggcggtgaat cagatgcact tccgaagcga tgttagacag
    16441 atacagcgcc tcttcaaccg cggtattgcc gccgccgatg accgcaactt tctggttgcg
    16501 atagaagaaa ccgtcgcagg tagcacaagc agaaacccca cggcctttaa aggcttcttc
    16561 agatggcagg cctagatagc gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt
    16621 gtattcgccg ctatcgccag tcagacggaa cggacgattc tgcaaatcaa ctttgttgat
    16681 gtgatcaaag atgatttcag tttcaaattt ggtggcatgt tcgtgcatgc gctccattaa
    16741 taacggaccg gtcagatcgt ttggatcgcc aggccagttt tccacttccg tggtggtggt
    16801 cagttggccg cctttttcca tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc
    16861 gtagacagca gcggtatatc ccgccgggcc tgaacccagg ataagcagtt tactgtgttt
    16921 ggtcgtgccc atgagatccc catagttgtt ggcagacaat gggcaggatt gtagggaatt
    16981 tacagacgta aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg
    17041 ataagcaggt caaattctcc cgtcattatc acctctgcta cttaaatttc ccgctttata
    17101 agccgattaa atgatgaata aacgcccctg ttaatgaata tctggcatgt tgtactaaaa
    17161 atcgatgttt tgctttgaca atcccctggt gttttgcgaa aacattcgag gaagaaaaaa
    17221 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
    17281 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
    17341 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
    17401 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
    17461 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
    17521 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
    17581 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
    17641 gggtttattc agggctatac ggcgctgctg aacccccatt atctggatgc atcacttctg
    17701 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
    17761 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatccggtga tttcgactac
    17821 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttactggg ggaaaccctg
    17881 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
    17941 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
    18001 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
    18061 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
    18121 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
    18181 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
    18241 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
    18301 tgcccggtgc gtggctggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
    18361 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
    18421 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
    18481 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
    18541 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
    18601 cgctgctctg cgtttgggct gcgggtctga cgctgttcac cggttggtca tgggtgacca
    18661 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
    18721 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagaa tatgaagatg
    18781 aaaatcacgg taaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgttagcgc
    18841 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gatgctgcgt
    18901 tgttctccgg taagcggatg gatgatgaag aagagattac ctacactgca cgcggtgtgg
    18961 ctgccgaccc tgacgacgtc ctcttttcgg gcaatcgtgc aacgcagcca gaatatgacg
    19021 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgttgctgta gcagctgctg
    19081 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
    19141 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
    19201 cacaaacggg agagccggtg attgctccgg caccggaagg ttacccacag cagccacaat
    19261 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca acctgtacag ccacagcagc
    19321 cgtattatgc gccagcagcg gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
    19381 aacaacctgt tcagcagccg tattatgcaa ctgcggcgga acagtctgcg caacagccgt
    19441 attacgcccc tgcgccagaa caaacggcgg caggtaacgc ctggcaagcc gaagagcagc
    19501 aatccacttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
    19561 ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcaccctgtt gtggagcctg
    19621 agcccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg
    19681 aagagaagcg tgcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attcctgaac
    19741 cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac
    19801 ctccagtgga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
    19861 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcgctg
    19921 gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
    19981 gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa gctgccctca cagcgtgcgg
    20041 cagaagaaaa agcccgtgaa gctcagcgca atcagtacga ttccggcgat cagtataacg
    20101 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
    20161 agcaacgcta tggcgaacag tatcaacatg atgtgcccgt gaacgcagaa gatgcagatg
    20221 ctgcggcaga ggctgaactg gctcgtcagt ttgctcaaac tcaacaacaa cgttattccg
    20281 gcgaacaacc tgctggggcg aatccgttca ctctggatga ttttgaattt tcgccaatga
    20341 aagcgttgct ggatgatggt ccacacgaac cgctgtttac gccaattgtt gaacctgtac
    20401 agcagccgca acaaccgatt gcaccgcagc aacagtatca gcagccgcag caaccagttg
    20461 cgccgcagcc gcagtatcag cagccgcaac agccggttgc accgcagcag caatatcagc
    20521 agccacaaca accggttgca ccgcagccgc aatatcagca gccgcaacag ccggttgcac
    20581 cacagccgca atatcagcag ccgcaacagc cagttgcgcc gcagccgcat gataccctgc
    20641 ttcatccgct gttgatgcgc aatggtgaca gccgtccgtt gcataaaccg acgacgccgc
    20701 tgccttctct ggatttgctg acgccgccgc cgagcgaagt ggagccggta gatacctttg
    20761 cgcttgaaca aatggcgcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
    20821 atgtcgtcaa ttattctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    20881 gagtaaaagc ggcgcgtatt tctaacttgt cacgtgacct tgcccgttca ctttcgacgg
    20941 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtagggctg gagttaccga
    21001 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    21061 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    21121 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aagtctgtcg
    21181 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    21241 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcatcccg catctgttaa
    21301 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    21361 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    21421 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    21481 gtgatagtat ggatgcgcat catccggtgc tgaaaaaaga accgtacata gtggtgttgg
    21541 tggacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    21601 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaacg cagcgtccat
    21661 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    21721 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    21781 gtatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    21841 cttttgtacg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    21901 cacagtatgt tgacggcatc acctccgaca gcgaaagcga aggtggtgcg ggtggtttcg
    21961 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    22021 gcaaagcgtc aatttcaggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    22081 gtattatcga acagatggaa gcgcaaggga ttgtcagcga acaggggcac aacggtaatc
    22141 gtgaagtgct ggccccaccg ccgtttgact aattaatgca tcgtaggccg gataaggcgc
    22201 ggtagcgtcg catccggcac tctaacaact gaaaattcag tattttcttc tttcctcaag
    22261 ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg
    22321 aggaataatg atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag
    22381 cagcgtttgg gctgatgccg caagcgatct taaaagccgc ctggataaag tcagcaactt
    22441 ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggcca
    22501 gggcgatctg tgggtgaaac gtccaaattt attcaactgg catatgactc aacctgatga
    22561 aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc
    22621 tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa
    22681 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac
    22741 gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac
    22801 aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc agctgaaatc
    22861 ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac
    22921 ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg
    22981 ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg
    23041 gccagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatt
    23101 tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga
    23161 ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc tgtcacctct ggcgtgaaag
    23221 agattcgcga ggcgattgag cgcgctcggc aaaaccgcaa tgcaggtcgc cgcactattc
    23281 tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata
    23341 ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg tttgaactta
    23401 actcggcact gctttcccgt gcccgtgtct atctgctgaa atccctgagt acagaggata
    23461 ttgagcaggt gcttactcag gcgatggaag acaaaacccg tggctatggt ggtcaggata
    23521 ttgttctgcc agatgaaaca cgacgtgcca ttgctgaact ggtgaatggc gacgcgcgcc
    23581 gggcgttaaa tacgctggaa atgatggcgg atatggccga agtcgatgat agcggtaagc
    23641 gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc cgctttgata
    23701 acaaaggaga tcgcttttac gatctgattt ccgcactgca taagtcggta cgtggtagcg
    23761 cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc gatccgttat
    23821 atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc gatccacggg
    23881 cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg
    23941 aacgcgccat tgctcaggcg attgtttacc tggcttgcgc gccaaaaagc aacgctgtct
    24001 acactgcgtt taaagccgcg ctggcggatg cacgcgaacg tccggattat gacgtgccgg
    24061 ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc
    24121 gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttatttc ccgccggaaa
    24181 tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa
    24241 agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt
    24301 atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca
    24361 tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag
    24421 acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg
    24481 ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta
    24541 actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac
    24601 gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt
    24661 tacaggctga aattcgcgat atcgcgctaa ccatccctaa cctgcctgca gatgaagtgc
    24721 cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggcacc ccgcgtgagt
    24781 ttgactttga agttcgtgac catgtgacgc tgggtgaaat gcactctggc ctcgactttg
    24841 cagccgcagt taagttgacc ggttcccgct ttgtggtaat gaaagggcag attgctcgca
    24901 tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg
    24961 agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga
    25021 aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact
    25081 atgcgctgat cccaacggca gaagttccgc tgaccaacct ggtgcgcggt gaaatcatcg
    25141 atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg
    25201 gctcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa
    25261 tggtgcagat cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg
    25321 cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg
    25381 acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca
    25441 cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg
    25501 cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg
    25561 gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc
    25621 gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat
    25681 acccaatttt tctgaatcta aaaagcgcct gcgggtggtt ttggtggtgt attaacatat
    25741 tgaaaataaa ggttttttat tttcatgtgt ccagataacg tccatctttg aaacaaaaag
    25801 cccgcagttt ttacgctgcg ggctttgttt ttctttgtg
//
LOCUS       DAZGTJ010000078        25830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000078 DAZGTJ010000000
VERSION     DAZGTJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.078"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            265..1563
                     /gene="kgtP"
                     /locus_tag="ACHK8E_003698"
     CDS             265..1563
                     /gene="kgtP"
                     /locus_tag="ACHK8E_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP0345912.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1560..1883)
                     /gene="yfiM"
                     /locus_tag="ACHK8E_003699"
     CDS             complement(1560..1883)
                     /gene="yfiM"
                     /locus_tag="ACHK8E_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP0345913.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1927..3282)
                     /gene="pssA"
                     /locus_tag="ACHK8E_003700"
     CDS             complement(1927..3282)
                     /gene="pssA"
                     /locus_tag="ACHK8E_003700"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP0345914.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3396..6056)
                     /gene="pat"
                     /locus_tag="ACHK8E_003701"
     CDS             complement(3396..6056)
                     /gene="pat"
                     /locus_tag="ACHK8E_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP0345915.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6088..6786)
                     /gene="tapT"
                     /locus_tag="ACHK8E_003702"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6088..6786)
                     /gene="tapT"
                     /locus_tag="ACHK8E_003702"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP0345916.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6855..7274)
                     /gene="trxC"
                     /locus_tag="ACHK8E_003703"
     CDS             complement(6855..7274)
                     /gene="trxC"
                     /locus_tag="ACHK8E_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP0345917.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7481..8518
                     /gene="yfiF"
                     /locus_tag="ACHK8E_003704"
     CDS             7481..8518
                     /gene="yfiF"
                     /locus_tag="ACHK8E_003704"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0345918.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8566..9255)
                     /gene="ung"
                     /locus_tag="ACHK8E_003705"
     CDS             complement(8566..9255)
                     /gene="ung"
                     /locus_tag="ACHK8E_003705"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP0345919.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            9560..9943
                     /gene="grcA"
                     /locus_tag="ACHK8E_003706"
     CDS             9560..9943
                     /gene="grcA"
                     /locus_tag="ACHK8E_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP0345920.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9999..10586)
                     /gene="eamB"
                     /locus_tag="ACHK8E_003707"
     CDS             complement(9999..10586)
                     /gene="eamB"
                     /locus_tag="ACHK8E_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP0345921.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10689..11570
                     /gene="yfiE"
                     /locus_tag="ACHK8E_003708"
     CDS             10689..11570
                     /gene="yfiE"
                     /locus_tag="ACHK8E_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0345922.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11603..12937)
                     /gene="srmB"
                     /locus_tag="ACHK8E_003709"
     CDS             complement(11603..12937)
                     /gene="srmB"
                     /locus_tag="ACHK8E_003709"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP0345923.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13069..13806
                     /gene="trmN"
                     /locus_tag="ACHK8E_003710"
     CDS             13069..13806
                     /gene="trmN"
                     /locus_tag="ACHK8E_003710"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP0345924.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13791..15413)
                     /gene="nadB"
                     /locus_tag="ACHK8E_003711"
     CDS             complement(13791..15413)
                     /gene="nadB"
                     /locus_tag="ACHK8E_003711"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP0345925.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15669..15824
                     /gene="rseD"
                     /locus_tag="ACHK8E_003712"
     CDS             15669..15824
                     /gene="rseD"
                     /locus_tag="ACHK8E_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP0345926.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15821..16396
                     /gene="rpoE"
                     /locus_tag="ACHK8E_003713"
     CDS             15821..16396
                     /gene="rpoE"
                     /locus_tag="ACHK8E_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP0345927.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16429..17079
                     /gene="rseA"
                     /locus_tag="ACHK8E_003714"
     CDS             16429..17079
                     /gene="rseA"
                     /locus_tag="ACHK8E_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP0345928.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17079..18035
                     /gene="rseB"
                     /locus_tag="ACHK8E_003715"
     CDS             17079..18035
                     /gene="rseB"
                     /locus_tag="ACHK8E_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP0345929.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18032..18511
                     /gene="rseC"
                     /locus_tag="ACHK8E_003716"
     CDS             18032..18511
                     /gene="rseC"
                     /locus_tag="ACHK8E_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP0345930.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18709..20508
                     /gene="lepA"
                     /locus_tag="ACHK8E_003717"
     CDS             18709..20508
                     /gene="lepA"
                     /locus_tag="ACHK8E_003717"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP0345931.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20524..21498
                     /gene="lepB"
                     /locus_tag="ACHK8E_003718"
     CDS             20524..21498
                     /gene="lepB"
                     /locus_tag="ACHK8E_003718"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP0345932.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21770..22450
                     /gene="rnc"
                     /locus_tag="ACHK8E_003719"
     CDS             21770..22450
                     /gene="rnc"
                     /locus_tag="ACHK8E_003719"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP0345933.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22447..23352
                     /gene="era"
                     /locus_tag="ACHK8E_003720"
     CDS             22447..23352
                     /gene="era"
                     /locus_tag="ACHK8E_003720"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP0345934.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23364..24092
                     /gene="recO"
                     /locus_tag="ACHK8E_003721"
     CDS             23364..24092
                     /gene="recO"
                     /locus_tag="ACHK8E_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP0345935.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24104..24835
                     /gene="pdxJ"
                     /locus_tag="ACHK8E_003722"
     CDS             24104..24835
                     /gene="pdxJ"
                     /locus_tag="ACHK8E_003722"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP0345936.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24835..25215
                     /gene="acpS"
                     /locus_tag="ACHK8E_003723"
     CDS             24835..25215
                     /gene="acpS"
                     /locus_tag="ACHK8E_003723"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0345937.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25637..25717
                     /gene="shoB"
                     /locus_tag="ACHK8E_003724"
     CDS             25637..25717
                     /gene="shoB"
                     /locus_tag="ACHK8E_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP0345938.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         6335 a         6425 c         6811 g         6259 t
ORIGIN      
        1 ccggatgcga cgctggcgcg tcttatactc ccacataagc tagattcagc agcgaatacg
       61 gcttccccaa cgtgcccacc tccatacgtg tcctccttac cagaaattta tccttaagct
      121 cctcaataac cattttcctg ctaactaaat tcgtggttaa ggttgcataa tgatatgcaa
      181 caaatgtata acattttctt taccaataga aataaacaaa agcgaccgac aaaagcatcg
      241 gattacggca ggagacataa tggcatggct gaaagtactg taacggcaga cagcaaactg
      301 acaagtagtg atactcgtcg ccgcatttgg gcaattgtgg gggcctcttc aggtaatctg
      361 gtcgagtggt tcgatttcta tgtctactcg ttctgttcac tctacttcgc ccacatcttc
      421 ttcccttccg ggaacacgac gactcaacta ctgcaaacag caggtgtttt tgctgcggga
      481 ttcctgatgc gcccaatagg cggttggcta tttggccgca tagccgataa acatggtcgc
      541 aaaaaatcga tgctgttatc ggtgtgtatg atgtgtttcg gatcgctggt tatcgcctgc
      601 ctcccaggtt atgaaactat aggtacgtgg gctccggcat tattgcttct cgctcgttta
      661 tttcagggat tatccgttgg cggagaatat ggcaccagcg ccacctatat gagtgaagtt
      721 gccgttgaag ggcgcaaagg tttttacgca tcatttcagt atgtgacgtt gatcggcggg
      781 caactgctag ctctactggt tgtcgtggtt ttacaacaca ccatggaaga cgctgcactt
      841 agagagtggg gatggcgtat tcctttcgcg ttaggagctg tgttagctgt tgtggcgttg
      901 tggttacgtc gtcagttaga tgaaacttca caacaagaaa cacgcgcttt aaaagaagct
      961 ggatctctga aaggattatg gcgcaatcgc cgtgcattca tcatggttct cggttttacc
     1021 gctgcgggct ccctttgttt ctataccttc accacttata tgcagaagta tctggtaaat
     1081 actgcgggaa tgcatgccaa cgtggcgagt ggcattatga ctgccgcatt gtttgtattc
     1141 atgcttattc aaccacttat tggcgcgctg tcggataaga ttggtcgtcg tacctcaatg
     1201 ttatgtttcg gttctctggc agccattttt accgttccta ttctctcagc attgcagaac
     1261 gtttcctcgc cttatgccgc ttttggtctg gtgatgtgcg ccctgctgat agtgagtttt
     1321 tatacttcaa tcagtggaat actgaaggct gagatgttcc cggcacaggt tcgcgcatta
     1381 ggcgttggtc tgtcatatgc ggtcgccaat gctatatttg gtgggtcggc ggagtacgta
     1441 gcgttgtcgc tgaaatcaat aggaatggaa acagccttct tctggtatgt gaccttgatg
     1501 gccgtggtgg catttctggt ttcactaaca ttgcaccgta aaggaaaagg gatgcggctt
     1561 tagtgacggg tcagttgcca gacggtatag ccggtgcttg caccggcgat atcccaggca
     1621 aaatccttcc agctccagcc gctcccctca gggcggctat cccaaagctc ttttgacgcc
     1681 cccagactga cagagaacat caatccaaac atggcactgc gatcccggct catcccctga
     1741 tgctgtgcat attcatttcc ggcggcggaa agcatcgccg aggcgataaa gtgttgagct
     1801 ttatcctgcc cgctccagct atcgttagcc atatggctgc atccagaaag taacaatagc
     1861 gaacagacaa aaagaatacg caaaacgtgg cctccaaaac aaaccccgta caggacgggg
     1921 ttgtgattac aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacgacggat
     1981 gagtttacga accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac
     2041 gatggtggta tgctcgcgga tcagctccag ttctttctct cgctgtggcg caagctccag
     2101 ttgcggatcg tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag
     2161 gttattaccg gtgatcaaca tccacttatc atcaacccac atccctttta ggtgataggt
     2221 gttgtcgtca tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa
     2281 acggctcagg aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt
     2341 gaaaggttca tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc
     2401 gacctttttc ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt
     2461 gaagtatggc gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt
     2521 cttgttcaac agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt
     2581 gtcggcatcg ccctggaaat gataagcggc atcacgcagc tcctggcgga acaggcgaat
     2641 gtcgttcttg atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg
     2701 gccattcata atattctgtg taacccattc aaacataatg tctgacatct tacggttacg
     2761 gattagatga taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag
     2821 gctggcaccg ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc
     2881 aagggcttca cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc
     2941 ctgcgccatg cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc
     3001 acgttgtgca cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt
     3061 agcctcatac aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc
     3121 aacaatgcaa atgcgctgct tcgcgctggc tattttttcc agcaacgtct cccggaagtc
     3181 ggcgggagcg taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc
     3241 aaggtgttgt tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc
     3301 tgttcattga agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag
     3361 tcccgattaa gtggtcaaca tttccagtac cttactcatg attcctcgcg ctgggcaaga
     3421 tttagcgtaa gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg
     3481 cgggccagcg ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt
     3541 ccgtgatctc gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct
     3601 ttgagatccg agcgaaccag cacggcaaat tcggcatcga tgttatcagg gtcggagatc
     3661 gcacgcgtga cgccgaggat ctcttctgtt tgatcaatac gtcgtaccgc cacaaaagcc
     3721 atttcccgat cgtagtcgat ctgcgtcatg ttggctaaat cttcatgggt aaattcgttg
     3781 atctcgctaa agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga
     3841 agctgtggct catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat
     3901 tctacccatt cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg
     3961 ccttcaaacg gcgcgatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc
     4021 agcaaaggat gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttagaaacc
     4081 tgcaccagaa gctggctcaa gcctgcaaca tctaatgggc gtagcgcact gcgcgcacga
     4141 atctttttac ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc
     4201 ggtagtgcga cgacggcttg atcttcagga cgccactcca caccgccttc gcccagcatg
     4261 atcaacggcc cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca
     4321 cggttagcca tactttgcac caacaggccg tggatccgcg cctgcggcca ggccattttt
     4381 acgcgatcga aaatagcgtt tgccgcttgc tggacttcat tagctgtacg caggtagagc
     4441 attacgccct gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc
     4501 ggataaccaa tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggtg
     4561 gggagcgtgt tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac
     4621 gtagcccctt cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attagaggtc
     4681 agattgctgg gcaacgccgg cgtttcgcgt agttgcttct gattacgtcg gtactccacc
     4741 atatgcataa aagcagtgat ggttccttcc ggcgtgcgat aggtcggcag cccggcttca
     4801 ctgaataatc gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcagagaa
     4861 acatacttgc tgcggggatg atgctttacc gcttcaatta atgcttgcgc actttctgtt
     4921 gcgggagcag cggcgctggg cgaatgaata accatcagcg cgtcaaaatc ctggctgtgg
     4981 agcagaatat ccagcgtttt aatatagtgc tcactgctgg cgtcatcgcg tagatcgagc
     5041 gggttagaga ttgccacatg ttctggcagt gcatcgcgca gtttctggca ggtttcttcg
     5101 cttagcgttg ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc
     5161 gcagcaccgt tgctgataat catcagccgg tcgccacgca gcgggcgcat atggctgagg
     5221 gtttccaccg ccgaaaacag ctcgtgagta tcctgtaccc gcaacaaacc ggcacgctga
     5281 atagccgcat cccacgccgg atccattcct gccgtggtgt tcaacagacg ttgagccgcc
     5341 gggctacgtc cgcttttaat caccagaatc ggtttgttgc gagaggcact acgggccgcc
     5401 gaaacaaagc gtcgtgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta
     5461 ctgtcgcggg ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc
     5521 gcaataaagt aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtattggag
     5581 acggcagccg attgcgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag
     5641 ctggcattca gaccttgcca gggagccagt aaccccagac tgtttggtcc tagcaggcgc
     5701 atgttatggc gcagggcgca ggcgcggaga tcttcatgtt gcgatgccgg ggcggaaaga
     5761 ataatgcagg ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg
     5821 gcattggtac ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag
     5881 gccaacacac ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag
     5941 cctcccgcca gcaggttacg catcatcagg taacctgctc gattggattt catcgatgcg
     6001 ccaattaccg caatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg
     6061 gtctcccgca gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct
     6121 acccagatgt tgcgtttttc ctgccagata gcgcgtttta aaacgagtga aatgctcgcc
     6181 taagcccgtt gcggcttcgg tatcgccggc catatctaac agtgcgatgg ctacctcggc
     6241 agtacaatat tggccttcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag
     6301 atcgacggaa atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc
     6361 ttcaggccag gtaccatcga gcatgataaa tagcggaggc ttaccggcag gtggtgtgaa
     6421 gatcacttcc cgttgctcat cagcatacga ggcgggaaag accaccattg gctgatagtc
     6481 cgggttttgt accagatcca gcaaatctag cgagggttcg gtacgcgacc attgaaacgc
     6541 aacggtatca ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc
     6601 ggtgtcgaac atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca
     6661 gagacataat gtctctggca aaagacagcg ttggcagcga cgaacgcgat taccgcgggc
     6721 aagaaaagga cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc
     6781 ggtcataaga gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat
     6841 gcgccccggt aagattaaag agattcgttc agccagctat caaacggcgc tttcggtact
     6901 gcgccattaa gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta
     6961 cgaattccaa agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc
     7021 actttaccgc tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg
     7081 ccgcaccacg gtgcccagaa gtcgatcacc acaggtagat catctttcag caatttgtcg
     7141 agcgtttcac cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg
     7201 cattttgccg catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta
     7261 caaacggtat tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg
     7321 tatgtttcta atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat
     7381 tcaataggta tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg
     7441 ggtaatctgc gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg
     7501 taaaagcggc aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac
     7561 ccacaacccg cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg
     7621 ccgtcgcccc gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagagtc
     7681 gccgtggcgc acagtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg
     7741 tggcatcagt ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga
     7801 aacccgcgtc tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcgat
     7861 tgttcgcgcc tggtttatcc agagtgtaac gccgcgtttt aaagaagcct tgcgctggat
     7921 ggcagcaaac cgcaaagcgt accatgtggt agatgaagcg gaactgacaa aagcgtcagg
     7981 cacggaacat cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgct
     8041 gcagtgggta agccaggcag gcgcgcagga ttgtgtactg gcgctggaaa acgaatctaa
     8101 cccgcataac ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggcgttgt
     8161 ggtgcaggat gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc
     8221 agagcacgtt cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca
     8281 ggcaggttac accgtagtga ccacttccag cgagcagggg aaaccgctgt tcaaaaccag
     8341 tctgccagcg aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc
     8401 acgcgatccg aacgatttgc gcgtgaagat tgatggtact ggcaacgttg ccgggctaaa
     8461 tatctctgtt gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata
     8521 aatcagccgg gtggcaacgc tgccatccgg cgttttcctg caaatttact cactttctgc
     8581 cggtaatact ggcatccagt caatcggttt ctcgccacgt tgttccagcc actgatttgc
     8641 cagcacaaaa tggttgcagc caaagaaccc acgatgcgca gaaagcggtg atggatgcgg
     8701 cgctttcagt atgtgatggc gttgcttatc aataatcgcc cctttctttt gcgcatgtga
     8761 gccccacaac aaaaacacca cgtcttcgcg gtgctggtta atcaggctga ttactttatc
     8821 ggtgaaggtc tcccagccga ggctggcgtg ggaatgtgcc tgacctgcgc gaaccgtcaa
     8881 cacagtattg agtagcagaa cgccctgacg cgcccagctt tcaagataac catgattagg
     8941 gcgggtgaaa ccgggaatag tattttccag ctctttatac atattcagca gtgacggcgg
     9001 tgtcgcaatg ccggggcgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata
     9061 aggatcctgg ccgagaatca ccactttaac gtcacccaac tctgtaaagc ggaacgcgtt
     9121 aaagacatct ttttgtggcg gatagatagt gacgccggac tgccgctcgc tggcgacggt
     9181 ctgtagggta ttaagaaaat agggttgctg cttctcttca gccagcacat catgccaggt
     9241 taattcgttt gccatctcgc tctcccgcga atcttcaatc cgcctagctt aactgcttct
     9301 ttctacagaa caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat
     9361 tggtaaaatt tcacacctgc ataagttgat gtaaaacaat aaaatccatt tgcaaccata
     9421 cttgatgtgt ggtttttatt gatttaaatc aaagattcaa gggtgtttga ggagtatata
     9481 tacactcaag caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat
     9541 tttgccgggg aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga
     9601 tctgctgaac tctttctggc tgctggacag cgaaaaaggc gaagcgcgtt gcatcgttgc
     9661 gaaagcaggt tttgcagaag acgaagtggt tgcagtaagc aaactgggtg acattgagta
     9721 ccgtgaagtt ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacatctgaa
     9781 cgtaaacgtt ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc
     9841 gcagctgacc atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca
     9901 gcagcgcgac gttattgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt
     9961 actaagcgga agagggcgac ttcttccgct ttttttcgtt aatagaaaat gcgtaccgcg
    10021 caatagacca gcagcagcgc gagcacgata tttaactggc gaccatactg gcgaaacaat
    10081 cgctgaaaca ggtgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc
    10141 agcaaaacgc tgacgccaat tatccagctt agcgcctgcg tttgcggcag gacaaacgtc
    10201 gacagtgctg taatgccata caaaatgatt ttgacgttca caaactgcaa agcaaagctg
    10261 gcccaaaaac tgattggttt tgtctgaagc ccatcttcct ttgttggact ggtagcgatt
    10321 ttccacgcca gccagacaat atatgccgcc cccgcccaac tcaacagatg cactgctgcc
    10381 gggtcgataa cagccagtga aaacgaaatt cccgcgcata gcaacatgac gattaaaaat
    10441 cccagactca tccctgccag cacgcgagta ctctgacgaa atccgtgcgt tgtggctgag
    10501 ctaagggcga gaatattgtt cggtcctggc gtcatggcgg taatcagggt gtaagtccaa
    10561 aaagcactta aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg
    10621 acacggaaca tttaaaaaaa tgaatgtttt tacattctaa attcgatatt ttcgataggt
    10681 ttggggttat ggatctgcgc cgttttatta cacttaaaac cgtggtggaa gagggttcct
    10741 ttttgcgagc ttcgcaaaaa ttgtgctgca cacaatcgac ggtgactttt catattcagc
    10801 agcttgagca ggagttctca gtccagttat ttgagaaaat tggtcgacga atgtgcctta
    10861 cccgcgaagg aaaaaagctg ctgccgcaca tttatgaact caccagagtg atggataccc
    10921 ttcgtgaggc tgccaaaaaa gagtcggatc cggacgggga actacgcgtt atttcaggcg
    10981 aaacgttgct ctcttatcga atgccgcagg tgttgcagcg ttttcggcaa cgcgctccaa
    11041 aagtgcgttt atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg
    11101 atgaggccga tgtcggggtt ttttatcgcg tagggaatga tgatgctttg aaccgacgag
    11161 agttgggtga acaaccgctg gtgctggtgg cttcaccgca aattgcagat gtcgatttta
    11221 ctgagccggg aagacataac gcctgtagct ttattatcaa cgaaccacaa tgtgtcttcc
    11281 ggcagatatt tgagagcacg ctgcgccagc gacggatcac ggtggaaaac accattgagc
    11341 ttataagtat cgaaagcatc aagcgttgtg tcgcggcaaa tatcggggtc agttacctgc
    11401 cgcgctttac agtggcaaat gaacttgcga gtggcgaatt aatcgaattg ccttttggcg
    11461 aacagtcgca gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg
    11521 cgatgcacac ttttattcag tgtgttgaag agtgtttttt gccgggataa aaacaatgcc
    11581 gggcttaaac ccggcattga gattactctt ctgtcgtttg cggtggcacg cccgttccgc
    11641 ttggtttacg gcgcttacca atatttttag tgtcgcgatg gcgttttttc acccgcggct
    11701 tctctttctc tttagctttt ttcttctcgg cacgtttagc caacactttc ttcgatggct
    11761 tgccggtctg cttctcgctt ggcgcacgcg ttttcggacg taactcatca attacgcgag
    11821 ctttaattgg ctcttcaata taacggccaa ctttgcccag cagcagatgg tcatgggctt
    11881 ccaccagcga aatcgcggtg cctttacgac cggcgcgcgc ggtacgtccg atacggtgca
    11941 aataagtatc gccactgcgc ggcatatcga agttaaagac gtggctgacg tcaggaatgt
    12001 cgataccgcg cgcggcaaca tcggtagcaa ccagtacgtt tacgcggcct tcggtcaagc
    12061 gtttaatcgc ttcgttacgc ttgccttgta ccatctcacc ttcgagatag cagttgttga
    12121 tgcctgcttc gcgcagccag tttgccagct catgcacacg ctcacgctta cgcacaaaca
    12181 caattgagcg ggtcgcttcc ggctgtttta acagatgcac cagcaacgcg gttttatgct
    12241 caagatcatc ggcgcggtaa taccactgat gaattttttt gcgctcacgg gtggacggat
    12301 tggcagaaac ctccaccgga tcttccagca gacgctcggc aaagtcctga atcgcatcgc
    12361 cttccagagt tgccgaaaag agcagggtct gtttacgcca gcgcgtttcg ccagcaatat
    12421 gttcgatatc ctgggcgaag cccatatcca gcatacggtc tgcttcgtcg aggatcagcg
    12481 tttcaaccgc gcggcaatcg aagttctctt cttttatgta ttgcagcaga cgtccggtcg
    12541 tggcgaccac gatgtcctga ttttcgctga acacttccgc gtggttcata taggctacgc
    12601 cgccggtgat ggtggcaata tccagatgcg tatgtttcgc cagttcgcgg gcatgatcgg
    12661 ccacctgcat cgccagctcg cgagttgggg tgaggatcaa aatacgcggc ggaccggatt
    12721 tcttacgtgg gaaatcgagc aggtgctgca acgctggcag cagatacgcc gccgttttac
    12781 cggtgcctgt cggcgcagaa ccgagtacat cacggccatc gagcgcaggc ggaatggcgg
    12841 cagcctgaat ggcggtcggg cgagtgaaac ctttatcctg gagggcttcc agcaggcttt
    12901 cgtcgagttc aagttcggaa aaagtcgtta cagtcatgtt ctacctctgt gtggggcgct
    12961 gattatagac gttacggctg caatcttcat ctgtttgtat ggatatcgct tctcgggtat
    13021 tgttctcacc cggtgctatc gccgtttttc ctgcaaggtt ttattttcat gtcacagtct
    13081 acatccgtgc ttcgtcgtaa tggatttact tttaaacagt tttttgttgc tcacgatcgt
    13141 tgtgcgatga aagtgggaac ggatggcatt ttgctgggcg catgggcacc ggtggctggg
    13201 gtaaaacgtt gccttgatat cggcgcgggt agcgggttgc tggcattaat gctggcgcag
    13261 cgaaccgatg acagcgtaat gattgatgca gttgaactgg aaagtgaagc tgccgcgcag
    13321 gcgcaggaaa atatcaacca gtccccgtgg gcagagcgga ttaacgtcca tacggcggat
    13381 attcagcagt ggatcacaca gcagacagta cgcttcgatt taatcatcag taacccacct
    13441 tactatcagc agggagtaga gtgcgcgaca cctcaacggg aacaggctcg ctataccact
    13501 acgcttgatc acccatcgtt gctgacctgt gcggcggagt gcattaccga agagggattt
    13561 ttctgcgtgg tgctgccaga gcagattggt aatggtttta ctgaactggc attaagtatg
    13621 ggctggcatt tacgtttgcg gacggatgtg gcggaaaacg aagcgcgact gccgcatcgg
    13681 gtgctgctgg cattctcccc gcaggcggga gaatgcttta gcgatcgctt agtcattcgt
    13741 gggccagacc agaactattc cgaagcgtat acggcgctga cccaggcttt ttatctgttt
    13801 atgtaatgat tgccggggga aaggatcgac ggaccggaat gggtgagcag ttccggataa
    13861 tccagcgtga aatgcaaccc ccgactctct ttacgcatca ttgcacagcg aacaatcaac
    13921 tcggcaacct gtaccagatt acgcagctcc agcaaattat ttgagacgcg gaaatgggcg
    13981 taatattcgt ctatttcttg ctggagcatg gttatccgcc gcagggcgcg ttccaggcgc
    14041 ttcgttgtgc gcacaatgcc gacgtaatcc cacataaaca gacgtagctc gtgccagtta
    14101 tgttgaatca ccacccgttc atcagggttc tcaacgcggc tttcatccca cggcggtaac
    14161 gtactgacgc cgtgggcata aggcatacat ctggtgatat cttctgccgc cgaccagcca
    14221 tagaccagac actccagcaa ggagttagag gccatgcggt tagcgccgtg taagccggta
    14281 taactcactt cgccaatggc atacaaaccc tcgacgtccg tacgcccatg atcatccacc
    14341 atcacaccac cgcaggtata gtgtgcagca ggcacaatcg gtactggttc ttgtgtgaga
    14401 tcaatcccca gcccgagcaa tttttcataa atcatcggga aatgctggcg aataaaatcg
    14461 gcgggcttat ggctgatgtc gaggaacata caatccgcgc cgaggcgttt catttcatgg
    14521 tcaatagcgc gggcgacaat atcgcgcggg gccagttcgc cgcgatcatc aaaatcgggc
    14581 ataaaacgcg taccatccgg gcgcttgaga taagcgcctt cgccgcgcag tgcttctgtt
    14641 aacaggaaat tgcgtgcctg tgggtgatac agtgcggtag ggtggaactg attaaattcg
    14701 agattggcaa cccggcagcc tgcgcgccac gccatagcaa tgccatcgcc agaagaaata
    14761 tccggattgg tggtgtactg ataaaccttc gacgcaccgc cggttgccag taccactgct
    14821 tttgcgtggc aggtttccac cgtttcttta tttcggttcc atacccacgc gccaacaacc
    14881 cgtcgcgtgc ccggcaggcc aattttgtca gaaacaatca gatcaaccgc gttgctgcgc
    14941 tccagcacgc gaatattcgg atggttcagc gccttgctca ccagcgtggt ttctacttct
    15001 ctaccggtgg cgtcggcggc atgaagaatt cgacggtgac tatgtccacc ttcacgggtc
    15061 agatggtagc tttctgcacc attcggttga atgtgggtat caaacaacac cccctggtcg
    15121 attagccatt gcacacagga tcgtgcattg ctggcgacaa attcaactgc atgacgatcg
    15181 caaataccag ccccggcaat caatgtgtct tccacatgcg agtcaatgct gtcagtctca
    15241 tcaaacacgg cggctatacc gccctgggca taaaatgttg aaccttccgt taccgggcct
    15301 ttacttagaa cgatgacctg atgctggtca gccaggcgta gcgccagtga aagtccagct
    15361 gcgccgctac cgataatcaa cacgtcacat gaatgttcag ggagagtatt cattttcttt
    15421 gtttaattta ctaaacatgg tttggtcagc atagcatcat gttgtgcgat taaacacctg
    15481 ctattttaat atttgttaca gtgactaaac acgctgactc agggcggcga gaaaaagaga
    15541 agttactggc tggtggagga ttaggtggtg aaataaaaag gccgttgggt tactcttcag
    15601 gcagttaaat gggcatttct acacagataa tgcgatgttc agattctgta gacttataat
    15661 gatagataat gatccgtcta cagcatgaca aacaaaaaca gatgcgttac ggaactttac
    15721 aaaaacgaga cactctaacc ctttgcttgc tcaaattgca gctaatggag tggcgtttcg
    15781 atagcgcgtg gaaatttggt ttggggagac tttacctcgg atgagcgagc agttaacgga
    15841 ccaggtcctg gttgaacggg tccagaaggg agatcagaaa gcctttaact tactggtagt
    15901 gcgctatcag cataaagtgg cgagtctggt ttcccgctat gtgccgtcgg gtgatgttcc
    15961 cgatgtggta caagaagctt ttattaaagc ctatcgtgcg ctggattcgt tccggggaga
    16021 tagcgctttt tatacatggc tgtatcggat tgctgtaaat acagcgaaaa attacctggt
    16081 tgctcagggg cgtcgtccac cttccagtga tgtggatgcc attgaagctg aaaacttcga
    16141 aagtggcggc gcgttgaaag aaatttcgaa ccctgagaac ttaatgttgt cagaagaact
    16201 gagacagata gttttccgaa ctattgagtc cctcccggaa gatttacgca tggcaataac
    16261 cttgcgggag ctggatggcc tgagctatga agagatagcc gctatcatgg attgtccggt
    16321 aggtacggtg cgttcacgta tcttccgagc gagggaagct attgataaca aagttcaacc
    16381 gcttatcagg cgttgacgat agcgggatac tggataaggg tattaggcat gcagaaagaa
    16441 caactttccg ctttaatgga tggcgaaacg ctggatagtg agctgcttaa cgaactggct
    16501 cataacccag aaatgcagaa aacctgggaa agctatcact taatccgtga ctcaatgcgg
    16561 ggtgatactc ccgaggtgct ccatttcgat atctcttcac gcgtgatggc cgccattgaa
    16621 gaagagccag tacgtcaacc ggcgacattg atcccggaag cccagcctgc gccgcatcaa
    16681 tggcagaaaa tgccattctg gcagaaagta cgtccgtggg cggcacagct tacccaaatg
    16741 ggcgtagccg catgcgtatc gcttgcagtt atcgttggcg tccagcacta taatggacaa
    16801 tctgaaacgt cccagcagcc cgaaacgccg gtatttaata cactgccgat gatgggtaaa
    16861 gccagcccgg taagcctggg agtaccttct gaagcgaccg caaacaatgg tcaacagcag
    16921 caggtacagg agcagcgtcg tcgcattaat gcaatgttgc aggattacga actgcaacgc
    16981 cgactccact ctgaacagct tcagtttgag caggcgcaaa ctcagcaagc cgctgtacag
    17041 gtgccaggaa ttcaaacttt aggaacgcaa tcgcagtaat gaagcaactt tggtttgcca
    17101 tgtcattagt gacaggtagc ctgttattct ctgctaacgc ctcggccact cccgcgtccg
    17161 gggcgttatt acagcagatg aacctggcca gtcagtcact gaattacgag ctgtcattca
    17221 tcagcatcaa taaacagggt gttgagtctc tgcgttatcg acatgcacgc ctcgataacc
    17281 gtcctcttgc acaattgttg caaatggatg gcccgcgccg ggaagtggta cagcgcggca
    17341 atgaaatcag ctattttgaa ccgggacttg aaccgttcac gcttaatggc gattacattg
    17401 ttgattctct accatcgctc atctataccg atttcaaacg cctttctcct tactacgact
    17461 ttatctccgt cgggcgcacg cgtattgctg atcgtctttg cgaagtcatt cgtgtggttg
    17521 cccgagatgg tacacgctac agctacatcg tgtggatgga caccgaatcg aaattaccga
    17581 tgcgggttga tctccttgat cgcgatggtg aaacgctgga acaatttcgc gtgattgctt
    17641 ttaatgtcaa tcaggatatc agcagcagta tgcagacgct ggcgaaggca aatttgccgc
    17701 cgttgctttc tgttcctgta ggtgaaaaag ctaaattcag ctggacgcca acctggttgc
    17761 cacagggttt tagcgaagtt tccagtagtc gacgtccgct accgacgatg gacaacatgc
    17821 ctatcgaatc acgtctctat tccgacggat tattcagctt ctcggtaaac gttaaccgcg
    17881 ctacgccatc gagcaccgat cagatgttgc gcaccgggcg cagaaccgtc agtacaagcg
    17941 tacgtgataa cgccgaaatc accattgtcg gtgaactgcc gccgcaaacg gcgaaacgca
    18001 ttgccgagaa tattaagttc ggggcagcgc aatgatcaaa gagtgggcta ccgtcgtctc
    18061 ctgggaaaac gggcaggcgc tggttagttg tgatgttaaa gcctcatgca gcagctgcgc
    18121 ttcacgtgcc ggttgcggta gccgcgtgtt gaataaactt ggcccgcaaa ccacgcatac
    18181 cattgtcgta ccctgtgatg agccgttagt gccggggcaa aaagtagaat taggaatcgc
    18241 cgaaggcagc ctgcttagct ccgcattact ggtttatatg tcgccgttgg tgggactatt
    18301 cctcatcgct tcactatttc agctactctt tgcttcagat attgcagcat tatgcggtgc
    18361 gattctcggt ggcgttggtg gattcctgat tgcacgtggc tgctctcgaa agtttgctgc
    18421 ccggtctgaa tggcaaccga tcatcttaag cgtggccttg ccgcctggcc ttgtgcgatt
    18481 tgaaacatct tcggaagacg cgagccagtg atttcactgg ctttgttgca ttttgcataa
    18541 caaatgcgag gacgtttcca gataccacaa tccatacggt atgaacattt cctcgcctca
    18601 atgttgtagt gtagaatgcg gcgtttctat taatacagac gttaagctca gaacagcgac
    18661 ttctaaaagc ctggttaacc gggcattaag gcacaataat catactttat gaagaatata
    18721 cgtaactttt cgatcatagc tcacattgac cacggtaaat cgacgctgtc tgaccgtatt
    18781 atccagatct gcggtggcct gtctgaccgt gaaatggagg cgcaggttct cgattccatg
    18841 gatcttgagc gtgagcgtgg cattaccatc aaagcgcaaa gcgtgacgct ggactacaaa
    18901 gcgtctgacg gcgaaaccta tcagcttaac tttatcgaca ccccgggcca cgtagacttc
    18961 tcctatgaag tttctcgttc gctggctgcc tgtgaaggtg cattgctggt ggtcgacgcc
    19021 gggcagggcg tagaagcgca aaccctggca aactgctaca ccgccatgga aatggatctc
    19081 gaagtggtgc cggtactgaa caagattgac ctgccggcag ccgatcctga acgcgtggcg
    19141 gaagaaattg aagatatcgt cggcatcgac gccaccgacg cggtgcgctg ttcagcgaaa
    19201 accggcgttg gcgtacagga cgttctcgaa cgtctggtgc gcgacattcc gccgccggaa
    19261 ggcgatccgg aaggcccgtt gcaggcactc attatcgact cctggttcga caactacctg
    19321 ggcgttgttt cacttatccg tattaaaaac ggcaccctgc gtaagggcga caaagtgaaa
    19381 gtcatgagta ccgggcagac ctataacgcc gaccgtctgg gcatcttcac gccgaagcag
    19441 gttgaccgta ctgaactgaa atgcggcgaa gtaggctggc tggtatgtgc gattaaagat
    19501 atccacggcg ctccagtagg cgatacctta acgctggcgc gtaatccggc agaaaaggcg
    19561 ctgcctggct ttaagaaagt caaaccgcag gtatacgccg gtctgttccc ggtaagttcc
    19621 gacgactatg aagccttccg tgacgcgctg ggcaaactca gcctgaacga tgcttcactg
    19681 ttctatgagc cggaaagctc cagcgcgctg ggctttggtt tccgctgcgg cttcctcggc
    19741 ctgctgcaca tggagatcat ccaggaacgt ctggaacgtg aatacgatct ggatctgatc
    19801 accactgcgc cgaccgtagt gtatgaagtt gaaaccacgt caagagaagt tatctacgtc
    19861 gacagcccat ccaagctgcc agcggtaaat aacatttacg aactgcgcga gccgattgca
    19921 gagtgtcaca tgctgctgcc gcaggcatat ctcggcaacg ttattacgct gtgcgtagaa
    19981 aaacgcggcg tgcagaccaa tatggtttac cacggtaatc aggtggcgct gacgtacgag
    20041 atcccgatgg cggaagtggt gctcgacttc ttcgatcgcc tgaaatctac ctcgcgtggt
    20101 tatgcgtctc tggattacaa cttcaaacgc ttccaggcgt ccgacatggt acgtgtagac
    20161 gtattaatca acggtgaacg tgttgatgcg ctggcgttga tcacccaccg tgataattcg
    20221 caaaaccgcg gtcgcgagct ggtggagaag atgaaagatc tgatcccacg ccagcagttt
    20281 gatatcgcca ttcaggcagc gattggtacg cacatcattg cgcgctccac cgtgaaacag
    20341 ctgcgtaaaa acgtactggc taaatgttat ggcggcgata tcagccgtaa gaaaaagctg
    20401 ctgcagaagc agaaagaagg taagaaacgc atgaagcaga tcggtaacgt cgagctgccg
    20461 caggaagcgt tcctcgccat tctgcacgtc ggcaaagaca acaaataacc cttaggagtt
    20521 ggcatggcga atatgtttgc cctgattctg gtgattgcca cactggtgac gggcatttta
    20581 tggtgcgtgg ataaattctt tttcgcacct aaacggcggg aacgtcaggc agcggcgcag
    20641 gcggctgccg gtgactcact ggataaagca acgctgaaga aagttgcgcc gaagcctggc
    20701 tggctggaaa ccggtgcttc tgtttttccg gtgctggcta tcgtattgat tgtgcgttcg
    20761 tttatttatg aaccgttcca gatcccgtca ggttcgatga tgccgactct gttaatcggt
    20821 gattttattc tggtagagaa gtttgcttat ggcattaaag atcctatcta ccagaaaacg
    20881 ctgatcgaaa ccggtcatcc gaaacgcggc gatatcgtgg tctttaaata tccggaagat
    20941 ccaaagcttg attacatcaa gcgcgcggtg ggtttgccgg gcgataaagt cacttatgat
    21001 ccggtctcaa aagagctgac gattcaaccg ggatgcagtt ccggccaggc gtgcgaaaac
    21061 gcgctaccgg tcacttactc caacgtggaa ccgagcgatt tcgttcagac cttctcacgc
    21121 cgtaatggtg gggaagcgac cagcggattc tttgaagtgc cgaaaaacga aaccaaagaa
    21181 aatggaattc gtctttccga gcgtaaagag acattgggtg atgtgacgca ccgcattctg
    21241 acagtgccga ttgcgcagga ccaggtgggg atgtattacc agcagccagg gcaacaactg
    21301 gcaacctgga ttgttccgcc gggacaatac ttcatgatgg gcgacaaccg cgacaacagc
    21361 gcggacagcc gttactgggg ctttgtgcct gaagcgaatc tggtcggtcg ggcaacggct
    21421 atctggatga gcttcgataa gcaagaaggc gaatggccga ctggtgtgcg cttaagtcgc
    21481 attggcggta tccattaata gccatcttcg ttcacgttgt cgccgttatg gcgacaacgt
    21541 gaattattta tgagataaat ctcccgtggc taacgacatc ccccgtcgtt gtgtatagaa
    21601 tattcccccg aagtttaagg ttggcacctc caggttgcca cggcacacga aacagcgttg
    21661 gtccccatat accggtaaac tgaaactgca gtgaagcagt tagccgagcc atgtatatca
    21721 ggtctgtttc gtgtgctgaa ttgttgacgc atttatttat tggtatcgca tgaaccccat
    21781 cgtaattaat cggcttcaac ggaagctggg ctacactttt aatcatcagg aactgttgca
    21841 gcaggcatta actcatcgta gtgccagcag taaacataac gagcgtttag aatttttagg
    21901 tgactctatt ctgagctacg ttatcgccaa tgcgctttat caccgtttcc ctcgtgtgga
    21961 tgaaggcgat atgagccgga tgcgcgccac gctggtccgt ggtaatacgc tggctgaact
    22021 ggcgcgcgaa tttgagttag gtgagtgctt acgtttaggg ccaggtgaac ttaaaagcgg
    22081 tggatttcgt cgtgagtcaa ttctcgctga caccgtcgag gcattaattg gtggcgtatt
    22141 cctcgacagt gatattcaaa ccgtcgagaa attaatcctc aactggtatc aaactcgtct
    22201 ggacgaaatt agcccaggcg ataaacaaaa agatccgaaa acgcgtctgc aggaatattt
    22261 gcagggtcgc catctgccgc tgccaactta tctggtagtc caggtacgtg gcgaagcgca
    22321 cgatcaggaa tttactatcc actgccaggt cagcggcctg agtgaaccgg tggttggcac
    22381 aggttcaagc cgtcgtaagg ctgagcaggc tgccgccgaa caggcgttga aaaaactgga
    22441 gctggaatga gcatcgataa aagttactgc ggatttattg ctatcgtcgg acgtccgaac
    22501 gttggcaaat ccacattgtt gaacaaactg ctggggcaga agatctccat cacctcccgc
    22561 aaggcgcaaa caactcgtca ccgcattgtg gggatccata ctgaaggcgc gtatcaggcg
    22621 atctacgttg ataccccagg cctgcatatg gaagaaaaac gcgccattaa ccgcctgatg
    22681 aacaaagcgg cgagcagctc tatcggcgac gttgagctgg tgatttttgt cgtagaaggc
    22741 acccgctgga cgccggacga tgaaatggtg ctcaacaaac tgcgcgatgg caaagcgccg
    22801 gtaatcctcg cggtgaacaa agtggataac gtgcaggaaa aagccgatct gctgccgcac
    22861 ttacagttcc tggcaagcca gatgaacttc ctggatattg tgccgatctc tgccgaaacc
    22921 gggctgaatg tcgacactat cgcagcaatc gtgcgtaagc atctgcctga agcgacccat
    22981 cacttcccgg aagattacat caccgatcgc tcacagcgtt ttatggcgtc tgaaatcatc
    23041 cgcgaaaaac tgatgcgttt cctcggcgct gaactgccgt actccgtgac cgtggagatc
    23101 gaacgtttcg tctctaacga acgcggcggt tatgacatca acggtttgat tctcgttgag
    23161 cgtgaagggc agaagaagat ggttattggc aacaaagggg ccaagatcaa aaccatcggg
    23221 attgaagcgc gtaaagacat gcaggaaatg tttgaagcgc ctgttcacct tgagctgtgg
    23281 gtaaaagtga aatccggttg ggccgacgac gaacgcgcac tgcgcagtct cggttatgtt
    23341 gacgatcttt aagagtaact ccgatggaag gctggcagcg cgcatttgtc ctgcatagtc
    23401 gcccgtggag cgaaaccagc ctgatgctgg acgtcttcac ggaggaatcg ggtcgcgtgc
    23461 gtctggttgc caaaggcgca cgctctaaac gctctactct gaaaggtgca ttacaacctt
    23521 tcacccctct cttgcttcgc ttcggtgggc gtggtgaagt caaaacactg cgcagtgctg
    23581 aagctgtctc gctggcgctg ccattaagcg gtatcacgct ttacagcggt ctgtacatca
    23641 acgaacttct ctcccgcgta ctggaatacg agacgcgctt ctctgaactc tttttcgatt
    23701 acttgcactg cattcagtca cttgcaggtg ccactggtac gccagaaccc gcgctgcgtc
    23761 gctttgaact ggcactgctc gggcatctgg gctatggcgt caattttacc cattgtgcgg
    23821 gtagcggcga gtcggtagat gacaccatga cgtatcgtta tcgcgaagaa aaagggttta
    23881 tcgcaagcgt cgttatcgac aataaaacgt tcaccggaag gcagttaaaa gcgttaaacg
    23941 cgcgggaatt tcctgacgca gacacactgc gcgccgcgaa acgctttacc cgcatggcgc
    24001 ttaagccgta tcttggcggt aaaccattaa agagcaggga actgttccgg cagtttatgc
    24061 ctaagcgaac ggtgaaaaca cattatgaat gatgaggatt gtcatggctg aattactgtt
    24121 aggcgtcaac attgaccata ttgcaacgct gcgtaacgca cgcggtactg cttacccgga
    24181 tccggtgcag gcggcgttta ttgctgagca ggcgggagcg gacggcatta ccgtgcattt
    24241 acgtgaagat cgccgccaca ttactgaccg cgatgtgcgc atcctgcgtc agacgctgga
    24301 tacccgcatg aatctggaga tggcggtgac cgaagagatg ctggcgatcg ccgttgagac
    24361 gaagccacat ttttgctgtc tggtaccgga aaagcgtcag gaagtaacaa ccgaaggcgg
    24421 cctggacgtc gcagggcagc gtgacaaaat gcgcgatgcc tgtaaacgtc tggcggaggc
    24481 cgggatcccg gtttctctgt ttatcgacgc cgatgaagag cagatcaaag ctgcggcaga
    24541 ggttggcgca ccgtttatcg agatccacac cggttgctat gctgatgcca aaactgacgc
    24601 cgaacaggcg caagagctgg cgcgtatcgc caaagccgcg acctttgccg ctagtctcgg
    24661 tctgaaagtt aacgctggtc acggtctgac ctatcacaac gtgaaagcta ttgccgccat
    24721 ccctgagatg catgaactga atatcggtca tgccattatt ggtcgtgcag tgatgaccgg
    24781 actgaaagat gcggtggcag aaatgaagcg tctgatgctg gaagcgcgtg gctaatggca
    24841 atattaggtt taggtacgga tattgtggag atcgctcgca tagaagcggt gatcgcccga
    24901 tccggtgagc gcctggcacg ccgcgtatta agcgataacg aatgggcaat ctggaaaacg
    24961 caccaccagc cggtgcgttt tctggcgaag cgttttgctg tgaaagaagc ggcggcaaaa
    25021 gcgtttggca ccgggatccg caatggtctg gcgtttaatc aatttgaagt attcaatgat
    25081 gagctcggca aaccacggct acggctatgg ggtgaggcat taaaactggc ggaaaagctc
    25141 ggcgttgtaa atatgcatgt aacgctggca gatgaacgcc actatgcttg tgccacggta
    25201 attattgaaa gttaaaaaaa taccgttctc tttataaagt aaaacccccg aactggtaag
    25261 gaacgggggt tttcacttta caactttaat tttgtgcagc acacgattcc aacagaaatg
    25321 attatggatc aatttcgtta tcctgtacaa gcaacttgca atctctgaca taaaaaacgc
    25381 gagcctttac gaagaaatcg acattgataa aaagattaac gtgaccgctt tttgataagt
    25441 acattaaaat cggtttcgtt attggcaatt ttgctgtgct ttacaccatg ccactgaatt
    25501 cccccattga aacgagtggt gtcgtcaaag ctctggtgtg gagtgcagca ctcgattcca
    25561 ataactcgca cgttcagttt tggggtgatg taagggctaa tctgaacggc tgcattcctt
    25621 gtttaaggaa aaacgaatga ctgattgccg atacctgatt aaacggatta tcaaaatcat
    25681 cattgctgtt ttacagctga tccttctgtt cttataacac aaggaaacgt acttaaggtg
    25741 cgtccggtga accagtcgga cgcgccttta ataactataa ataagtgtct gggcagatac
    25801 tatataaatt aacttagtga ataattatgc
//
LOCUS       DAZGTJ010000079        25521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000079 DAZGTJ010000000
VERSION     DAZGTJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.079"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(102..185)
                     /gene="yohP"
                     /locus_tag="ACHK8E_003725"
     CDS             complement(102..185)
                     /gene="yohP"
                     /locus_tag="ACHK8E_003725"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP0345939.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            409..1845
                     /gene="mdtQ"
                     /locus_tag="ACHK8E_003726"
     CDS             409..1845
                     /gene="mdtQ"
                     /locus_tag="ACHK8E_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP0345940.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1898..2659
                     /gene="yohF"
                     /locus_tag="ACHK8E_003727"
     CDS             1898..2659
                     /gene="yohF"
                     /locus_tag="ACHK8E_003727"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0345941.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2778..3356)
                     /gene="yohD"
                     /locus_tag="ACHK8E_003728"
     CDS             complement(2778..3356)
                     /gene="yohD"
                     /locus_tag="ACHK8E_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0345942.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3526..4113
                     /gene="yohC"
                     /locus_tag="ACHK8E_003729"
     CDS             3526..4113
                     /gene="yohC"
                     /locus_tag="ACHK8E_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP0345943.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4287..5219
                     /gene="pbpG"
                     /locus_tag="ACHK8E_003730"
     CDS             4287..5219
                     /gene="pbpG"
                     /locus_tag="ACHK8E_003730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP0345944.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5258..6973)
                     /gene="dld"
                     /locus_tag="ACHK8E_003731"
     CDS             complement(5258..6973)
                     /gene="dld"
                     /locus_tag="ACHK8E_003731"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0345945.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEYLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            7169..9466
                     /gene="bglX"
                     /locus_tag="ACHK8E_003732"
     CDS             7169..9466
                     /gene="bglX"
                     /locus_tag="ACHK8E_003732"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP0345946.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9615..10532
                     /gene="osmF"
                     /locus_tag="ACHK8E_003733"
     CDS             9615..10532
                     /gene="osmF"
                     /locus_tag="ACHK8E_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP0345947.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10539..11696
                     /gene="yehY"
                     /locus_tag="ACHK8E_003734"
     CDS             10539..11696
                     /gene="yehY"
                     /locus_tag="ACHK8E_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP0345948.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11689..12615
                     /gene="yehX"
                     /locus_tag="ACHK8E_003735"
     CDS             11689..12615
                     /gene="yehX"
                     /locus_tag="ACHK8E_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP0345949.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            12620..13351
                     /gene="yehW"
                     /locus_tag="ACHK8E_003736"
     CDS             12620..13351
                     /gene="yehW"
                     /locus_tag="ACHK8E_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP0345950.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13332..13439)
                     /gene="yohO"
                     /locus_tag="ACHK8E_003737"
     CDS             complement(13332..13439)
                     /gene="yohO"
                     /locus_tag="ACHK8E_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP0345951.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13499..14230)
                     /gene="mlrA"
                     /locus_tag="ACHK8E_003738"
     CDS             complement(13499..14230)
                     /gene="mlrA"
                     /locus_tag="ACHK8E_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP0345952.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14452..16137
                     /gene="btsS"
                     /locus_tag="ACHK8E_003739"
     CDS             14452..16137
                     /gene="btsS"
                     /locus_tag="ACHK8E_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP0345953.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            16134..16853
                     /gene="btsR"
                     /locus_tag="ACHK8E_003740"
                     /gene_synonym="yehT"
     CDS             16134..16853
                     /gene="btsR"
                     /locus_tag="ACHK8E_003740"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP0345954.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16900..17370
                     /gene="yehS"
                     /locus_tag="ACHK8E_003741"
     CDS             16900..17370
                     /gene="yehS"
                     /locus_tag="ACHK8E_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP0345955.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17412..17873)
                     /gene="yehR"
                     /locus_tag="ACHK8E_003742"
     CDS             complement(17412..17873)
                     /gene="yehR"
                     /locus_tag="ACHK8E_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP0345956.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17998..20001)
                     /gene="yehQ"
                     /locus_tag="ACHK8E_003743"
     CDS             complement(17998..20001)
                     /gene="yehQ"
                     /locus_tag="ACHK8E_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP0345957.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19998..21134)
                     /gene="yehP"
                     /locus_tag="ACHK8E_003744"
     CDS             complement(19998..21134)
                     /gene="yehP"
                     /locus_tag="ACHK8E_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0345958.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(21127..23406)
                     /gene="yehM"
                     /locus_tag="ACHK8E_003745"
     CDS             complement(21127..23406)
                     /gene="yehM"
                     /locus_tag="ACHK8E_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP0345959.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23417..24505)
                     /gene="yehL"
                     /locus_tag="ACHK8E_003746"
     CDS             complement(23417..24505)
                     /gene="yehL"
                     /locus_tag="ACHK8E_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP0345960.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5970 a         6464 c         6949 g         6138 t
ORIGIN      
        1 tgcgccagtg attatatgaa gcaatgcaca tgacaggagc gcaacaggat gagagactga
       61 aatattctcg ttattttcgg atctgatgac attaattaat tttaaaatat catcttaaat
      121 acgccagtca ccaccagtag cccaatcagg aaaataatca atacagccca gagtataatt
      181 ttcattggcg ttcctttatt tgtgagtgct gaataagtat aggaaaaaac gaagcccttt
      241 gccgctatcc ataaacgatt aagataaatc ggaagatatc attcacttgg tgtatatttt
      301 tctggatatg tcgttttatt gccactgatg tattgccacg cgaaactaaa aaatatatcg
      361 caacacacgg taatctccgc gcaccttttc ttacctgatt attcccccat gaatcgtgat
      421 tccttttatc cagccatcgc ctgttttccg ctgttactga tgctggccgg gtgtgcgcct
      481 atgcatgaaa cccgccaggc gttaagccag cacacgcccg ctgtgcaagt tgacaccgca
      541 ttacccacgg cgctgaaaaa tggttggcca gacagccaat ggtggctgga gtatcacgat
      601 aatcaactca cctcattaat taataacgca ctacagaacg caccggttat gcaggtggct
      661 gaacagcgca ttcagctggc tgaagcccag gcgaaggcaa ttgctactca ggatggtccg
      721 cagctcgact tttctgcgga tatggagcgg caaaaaatgt cggcagaagg cttaatgggg
      781 ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc cgtggtacac caacggtact
      841 tttggcttaa cggcgggctg gcatctcgat atctggggaa agaatcgggc ggaggtaact
      901 gctcgcctgg gtacggttaa agcacgagcg gcggaacgcg agcaaacccg ccaattgctg
      961 gctggcagcg tagcccgcct gtactgggag tggcaaaccc aggcggcgtt aaacacggtc
     1021 ttgcagcaaa tcgaaaaaga gcagaacacc attatcgcca ccgatcgcca gctatatcag
     1081 aacgggatta cttcttcagt tgaaggtgtg gaaaccgata ttaatgccag caaaacccgg
     1141 cagcagctca acgatgtcgc ggggaaaatg aaaattattg aggcacggtt aagtgcactt
     1201 acaaatcacc agacaaaggc attgaagctt aatccggtcg cgttgccgaa agtggcaagc
     1261 cagcttcctg atgaactggg gtactcctta ctggcccggc gggcagattt gcaggcggcg
     1321 cactggtacg ttgagtcatc gttaagcact attgatgcgg caaaagcggc attttatcct
     1381 gacatcaacc tgatggcctt cctgcaacag gatgcgttgc acttaagcga tctgttccgt
     1441 cattccgcgc agcaaatggg cgttacggca ggcctgacgc tacccatttt cgatagtggt
     1501 cgtcttaacg ccaacctcga tatcgcaaaa gccgaaagca acttgtctat cgccagctac
     1561 aacaaagcgg tggttgacgc ggtgaatgac gtggcgcggg cagccagtca ggttcagaca
     1621 ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg atgccttgcg tgtagtaggt
     1681 cttgcacagg cgcgctttaa cgcgggcatc attgccggtt cccgcgtcag cgaagccaga
     1741 atcccggcgc ttcgcgagcg ggccaatggc ctgttattgc aagggcagtg gctggatgcc
     1801 tccattcaac tcactggtgc gttgggcggg ggttacaaac gctgatgata tgcgccttct
     1861 atacttaacg tttattcagc gttaagtgga gaactcgatg gcacaggttg cgattattac
     1921 cgcctccgat tcggggatcg gcaaagagtg cgcgttatta ctggcgcagc aggggtttga
     1981 tattggcatt acctggcact ctgatgaaga aggggcaaaa gatactgcgc gtgaggtggt
     2041 tagccacggc gcacgtgcgg agatcgtgca gctggatctc ggtaaactgc cagaaggtgc
     2101 gcaggcgctg gagaaactca ttcaacgact ggggcgcatc gatgtgctgg tgaataatgc
     2161 gggtgcaatg accaaagcgc cgtttcttga tatggctttt gatgagtggc gcaagatttt
     2221 taccgttgat gtcgacggtg cattcttatg ctcgcaaatt gcggctcgtc agatggtgaa
     2281 acaagggcag ggcggtcgca tcatcaacat tacgtcggtg catgaacata cgccgctgcc
     2341 ggatgccagc gcttacacag cagctaaaca tgcgctcggc gggttaacca aagcaatggc
     2401 gctggagttg gtcaggcata aaattttggt gaacgcagtc gcacctgggg cgatcgccac
     2461 gccaatgaac ggcatggatg atagcgatgt gaggcccgac gcggagcctt cgattccctt
     2521 gcgacgtttt ggcacaacgc atgagattgc cagcctggtg gtatggcttt gttcggaggg
     2581 cgcaaattac accaccgggc agtcgttgat agtggatggc ggctttatgt tggcgaatcc
     2641 gcagttcaac ccagaatagc gcagtgaggt gcgtggttat ttcaatgggt taggccagat
     2701 aagacacgcc agcgtcgcat caggcatgga acatccggtg ccggatgtgg cgtgaatgct
     2761 tatctgtggc aactgcttta cgcctgatta tccggctttt tcttcccgcg tcgtttcagc
     2821 caccagcgca cgcctaccac cagaaccacg gccagaatca accagaccca gtgcttcaaa
     2881 tgctggtcga gattgtgcaa ccacggcgca atcacctgac caccagcata accgatagtg
     2941 gtaaaaatca acgcccaggc aaatgcgccg agaatattca gcggcagaaa gattttcggc
     3001 ggcagctggc tggcaccaat cagcgtcggg ccaatcaccc taaagccata cataaaacgc
     3061 gtaccaatga caaacagata cggatggcgt tggataagtt tctgcgcccg ctcaatttta
     3121 tcctgatgtt tcgagaaacg gcgtaatagc ttgccgccaa agcgccgccc gcacagataa
     3181 agcacctggt cgccaatcat gccgccaagc gccacagaaa gtaccaccag cgggaacttt
     3241 aatagcccct gatgtgccgc aacgcctccc agcaaagtca cggtttcacc ttccgccagg
     3301 ctaccgatca ccagcgcggc ataaccatat tgtgagatga gtgtattgag atccataggc
     3361 cagtctgtct ccctttgctt gcctgtaagc atacaccctg tgaatagttc acgctgaaaa
     3421 aggacgtaca tcagacggtg caaattacat gagcgaacaa aaaataatca caagctgact
     3481 tatacttgaa ctgtcaggtt catgaactca caacaagggg gcggtatgag ccatgtctgg
     3541 ggactgtttt cccatcccga tcgtgaaatg caggtgatta atcgcgaaaa cgaaacgatt
     3601 tctcaccatt acacccacca cgttttgctg atggcggcga ttccggtgat ttgcgccttc
     3661 attggcacta cacagattgg ctggaatttt ggcgatggca ctatcctgaa gttatcctgg
     3721 tttaccggac tggcgctggc tgtcttgttt tacggcgtta tgctggctgg ggttgcggtg
     3781 atggggcggg tcatctggtg gatggcgcgt aattatccgc agcgtccgtc acttgcgcac
     3841 tgcatggtct tcgcgggcta cgttgcaact ccgctgtttt taagcggtct ggtggcgctt
     3901 tacccgctgg tctggttgtg tgcgctggtc ggcacggtgg cgctgtttta caccggctac
     3961 ctgctgtatc tcggtattcc gagcttcctg aatatcaaca aagaggaagg acttagcttc
     4021 tccagttcga cactggcgat aggcgtgctg gtgctggaag tgctgctcgc ccttaccgtt
     4081 atactatggg gctacggtta tcgtttgttc tagaaagttc ttttgcgctg cattgctggc
     4141 gtaaatgtca gcaatgcagc atttcctcac gattcttctt tgacgatcgc actttttgct
     4201 cgttatgatg gcgtcgctag cctcagtaaa tccttactcc ggcggtgcgc aacccgtgcg
     4261 cgtgaaccac tatctgaatg ctcatcatgc cgaaatttcg agtttcttta tttagcctgg
     4321 ccctgatgct ggctgtgcct ttggcaccgc aggccgttgc gaaaacggca gccgctacca
     4381 ccgcttcaca accggaaatt gcctccggta gcgcgatgat tgttgatctg aataccaaca
     4441 aagtgatcta ttcgaaccac ccggatctgg tgcgtccgat tgcgtctatc agcaaattaa
     4501 tgaccgcgat ggttgtgctg gatgcacgac tgccgctgga tgaaaaacta aaagtggata
     4561 tcagccagac gccggagatg aaaggggtct attcgcgcgt acgactgaac agcgaaatca
     4621 gccgtaaaga tatgctgttg ctggcgctga tgtcttcaga aaaccgcgcg gcggcaagcc
     4681 ttgcgcacca ttatcccggt ggttacaaag cctttattaa ggcgatgaat gcgaaagcga
     4741 aatcgctcgg aatgaacaac acgcgctttg ttgaaccgac cggattgtcg gtgcataacg
     4801 tttcaactgc ccgtgactta accaaactgc tcattgccag caaacaatat ccgttgattg
     4861 gtcagttaag tactaccagg gaagatatgg caaccttctc taacccgacg tatacgctgc
     4921 cgttccgcaa tactaaccat ctggtgtatc gcgataactg gaatattcag ttaaccaaaa
     4981 cgggctttac caatgcggcg ggccattgtc tggtgatgcg tacggttatc aataataaac
     5041 cggtggcgct ggtagtgatg gacgcgtttg gcaaatatac ccattttgcc gatgccagcc
     5101 gcctgcgtac ctggattgag accggtaaag tgatgcctgt tccggcagca gcgttgagct
     5161 ataaaaaaca aaaagccgcc caaatggcgg cggcggggca gacggcacag aacgattaaa
     5221 tgaatgacgg atggcgatac tctgccatcc gtaattttta ctccacttcc tgccagtttt
     5281 tccgcttact ggttttacca acccccggat tcatactgtt ggtcggatcg ttctcgcgat
     5341 aaaacttctg caacgtttcc ggtgctttat acaaatgacc gacgttatgc tcggcagggt
     5401 attgcgcgcc gcgctgctgt agcagttcca gcatctgttc tttcaacgca tgcacatcca
     5461 cgcctttttt aacgatgtaa tcctgatgga agacatagca cataaaatgg ccgtaataga
     5521 gcttgtgcac cagctggctg tcgatctccg gcggtaaata ctcataccac tcggtgtcgt
     5581 tacgccgcag agcgatatcc agtgccagaa tgtcttcgac ttcatcggaa tgcaccgcct
     5641 gataacgaat tgctgcgccc gcagcggcga aacggtgtaa aaacgctttg ctgccttcct
     5701 ccggcgtaca gacaaagaaa tcgccttcgg cctgtttgaa ataatccacc agccacgatt
     5761 tggcttcacc gacgccatcg cccgccattt ttaacagcag atgatgttcg tatttatcgc
     5821 gccagttttt catgcgcggc ggtaaatggc tggggaacag gtgaccgaat ttttgcatcg
     5881 cacggtcggt aaaatgcgga cggaagaatt tcactttctc cagcatcgca tcggtgcgcc
     5941 ccttgagatt aaagaagaac ggcatcttgt cggtgcctaa cttatcaatc atcaggaagg
     6001 tgtctttgcc gtatttttcc gctatatcgt agatatcccg gtgcatatat tccccggcaa
     6061 ccggcagatt ttcgaaatta gccagaatat gacggcggat ttcggtcagt acttccggct
     6121 ggttggtgcc gatataaaac acctgctgat ttttttccgc ttcgaaggta tcaagacgta
     6181 ccgcaaagac ggccagcttc ccggcgcaac cagaagattc aaataaccga tccggatcgg
     6241 cgttataacg tgcgggcgtg tcggcttcaa tatcacgaac gcggtggaca tagtcataat
     6301 cgtgggcgtg acgaccatcg tggcgcacat cgtcatcttt gatgcgatcg tcatccagct
     6361 tgctaaggat ctgttccggc gtttcgccca gatcaatccc cagatggtta accagcgtca
     6421 gcttgccatc ttcatttata cgcgcgaata acgacatttc ggtatacgcc gggccacgtt
     6481 gcaccagcga gccgccggag ttgttacaaa taccgccgat gaccgatgcg cctatacacg
     6541 atgatccaat caccgagtgc ggttcgcgtc ccagcggttt gagggctttt tccagcgaat
     6601 aaagcgtggt gcccggatag gccagcacct gttcgccctt gccgagaacg tgcagcttgt
     6661 cgagacgcag ggtgctgatg atcacgatat cgcgatcata gtcgttaccg tttggcgtcg
     6721 atccttcggt caggcctgta ttggcagcct gcatcagaat aattttgtcg gcggtgacgc
     6781 aggctttcag cacccgccac aattccagta gtgagccagg gaaaacgaca gccagtgcgt
     6841 cgccctgacc agaacggaag cccttgcgat agcgggccgt ttttgcggga tcggtgagca
     6901 ggtgtgaatg accgaccaga cgggcaagtt cattcaaaaa ggctttatta tcagttgttg
     6961 tcatggaaga cattttccac tccttgtggt ggcgaaaaaa tatcacaaac tagaatagcg
     7021 ccttgtatga caattcagcg agtgaatatt ttgaaaattc agacaataaa ctgagcattc
     7081 tttttctctc tccatcatgc ttatggcaaa ctacggtttt tgcaccattt tcgctgccac
     7141 gtcgggcaac aaaggaagaa aaatccatat gaaatggcta tgttcagtag gaatcgcggt
     7201 aagtctggcc ctgcagccag cactggcgga tgatttattc ggtaaccatc ccttaacgcc
     7261 tgaagcgcgg gatgcgttcg tcaccgaact gcttaagaaa atgacggttg atgagaaaat
     7321 tggtcagctg cgcttaatca gcgtcggccc ggataatccg aaagaggcga tccgcgagat
     7381 gatcaaagac gggcaggttg gggcgatttt caacaccgta acccgtcagg atatccgcgc
     7441 catgcaggat caggtgatgg aattaagccg cctgaaaatt cctcttttct ttgcttacga
     7501 cgtgctgcac ggtcagcgca cggtgttccc gattagcctc ggtctggcct cgtcttttaa
     7561 cctcgatgcg gtgaaaacgg tcggacgtgt ctctgcttat gaagcggcag atgatggcct
     7621 gaatatgacc tgggcaccga tggtcgatgt ctcgcgcgat ccgcgctggg gacgtgcttc
     7681 cgaaggtttt ggcgaagata cgtatctcac ttcaacaatg ggtaaaacca tggtggaagc
     7741 gatgcagggt aaaagcccgg cagatcgtta ctcggtgatg accagcgtca aacactttgc
     7801 cgcatacggc gcggtagaag gcggtaaaga gtacaacacc gtcgatatga gtccgcagcg
     7861 cctgtttaat gattatatgc cgccgtacaa agcggggctg gacgcaggca gcggcgcggt
     7921 gatggtggcg ctgaactcgc tgaacggtac gccagccacc tccgactcct ggctgctgaa
     7981 agatgttctg cgcgaccagt ggggctttaa aggcatcacc gtttccgatc acggcgcaat
     8041 caaagagctg attaaacatg gcacggcggc agacccggaa gatgcggtgc gcgtggcgct
     8101 gaaatccggc atcaacatga gtatgagcga cgagtattac tcgaagtatc tgcctgggtt
     8161 gatcaaatcc ggcaaagtga cgatggaaga gctggatgac gctgcccgtc atgtactgaa
     8221 cgttaaatat gatatggggt tgtttaacga cccatacagc catttggggc cgaaagagtc
     8281 tgacccggtg gataccaatg ccgaaagccg cctgcaccgc aaagaagcgc gtgaagtggc
     8341 acgcgaaagc ctggtgttgc tgaaaaaccg tctcgaaacg ttaccgctga aaaaatcagc
     8401 caccattgcg gtggttggcc cgctggcaga cagcaagcgt gacgtgatgg gaagctggtc
     8461 cgcagccggt gtcgccgatc aatctgttac tgtgctaaca gggattaaaa acgccgtcgg
     8521 tgaaaacggt aaagtgctgt atgccaaagg ggcgaacgtt accagtgaca aaggcattat
     8581 cgatttcctg aatcagtatg aagaagcggt caaagtcgat ccgcgttcgc cgcaagagat
     8641 gattgatgaa gcggtgcaga ccgcgaaaca atctgatgtg gtggtggctg tagtcggtga
     8701 agcacagggg atggcgcacg aggcttccag ccgtaccgat atcactattc cgcaaagcca
     8761 acgtgacttg attgcggcgc tgaaagccac cgctaaaccg ctggtgctgg tgctgatgaa
     8821 cgggcgtccg ctggcgctgg tgaaagaaga tcagcaggcg gatgcgattc tggaaacctg
     8881 gtttgcgggg actgaaggcg gtaatgcaat tgccgatgta ttgtttggtg attacaaccc
     8941 gtccggcaag ctgccgatgt ccttcccgcg ttctgtcggg cagatcccgg tgtactacag
     9001 ccatctgaat accggtcgcc cgtataacgc cgataagccg aacaaataca cgtcgcgtta
     9061 ttttgatgaa gctaacgggg cgctgtatcc gttcggctat ggtctgagct acaccacttt
     9121 caccgtctct gatgtgaaac tttccgcgcc gaccatgaag cgtgacggca aagtgaccgc
     9181 cagtgtgcag gtgacgaaca ccggtaagcg cgaaggggcg acggtagtgc agatgtactt
     9241 gcaggatgtg acggcttcca tgagtcgtcc ggtgaagcag ctgaaaggct ttgagaaaat
     9301 caccctgaaa ccaggcgaaa cccagaccgt cagcttcccg attgatatcg aggcgctgaa
     9361 gttctggaat caacagatga aatatgacgc cgagcctggc aagttcaatg tctttatcgg
     9421 cactgattcc gcacgcgtta agaaaggcga gtttgagttg ctgtaatgct ttagtaaggg
     9481 cgcgacgtct gcgcccttta agtatttatc tgattctgca actgaatccg cccagacagg
     9541 ctaaccctgt cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
     9601 atgaggaaag catcatgcca ctctcaaagc tctgggcagg ttcattggtt ttgttggcag
     9661 ccgtgagcct gccgctacag gcggcttccc ccgttaaggt cggttcaaaa atcgataccg
     9721 aaggcgcgct gctcggcaat atcattttgc aggtgctgga aagtcacggt gttcctacgg
     9781 taaataaagt gcaactggga acgactcccg tggtgcgcgg ggcgattact tccggtgaac
     9841 tggatatcta tccggaatat accggcaatg gcgcgttttt ctttaaagat gaaaacgatg
     9901 cggcatggaa aaatgccggg caaggctacg agaaagtcaa aaaactcgat gcagagcaaa
     9961 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    10021 aggatgtggc agagaaaaac aaactcactt cgcttgccga cctgagtcgt tatctgaaag
    10081 agggcggcac cttcaaactg gcggcctcgg cagaatttat tgaacgcgcc gatgccttac
    10141 ccgcttttga aaaagcctac gactttaaac tcgatcagga tcagttgctg tcactggctg
    10201 gtggcgacac ggcggtaacg atcaaagccg ctgcccagca aacttctggc gttaatgccg
    10261 caatggctta cggcactgac ggtccggtcg cagcgctggg gttgcaaacc ttaagcgatc
    10321 cgcaaggcgt gcaacctatc tacgcgcctg caccagtggt tcgtgaatcg gtgttgaagg
    10381 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    10441 tgcagcaact gaatgccagc attgcagttg aaggactgga tgccaaaaaa gtggctgccg
    10501 actacctgaa acaaaaaggg tggacgaagt aatttcccgt gacttatcta cgtattaatc
    10561 ctgttctggc gctgctgctg ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt
    10621 acgcgcctaa tcgtttagtt tcgggtgagg ggcgtcatct ctggcagctg tggccgcaaa
    10681 cgatctggat gctggtgggc gttggttgcg cctggctgac cgcctgtgtt attcccggta
    10741 aaaaaggcag catttgtgca ctcattctgg cgcaattcgt cttcgtattg ctggtgtggg
    10801 gagctggaaa ggcggcgaca caactggcac aaaatggcag tgcgctggcg cgtaccagcc
    10861 tcggcagtgg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    10921 gccgaatctc cacgcatctg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    10981 cgctgtggtt gctgtactcc ggcacgctta acgatctctc gctaatgaaa gaatacgcca
    11041 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    11101 tgctgcctgc gttagtgatt ggtgtgccgt tgggcgtctg gtgctacttt tccactgcgc
    11161 ggcagggagc gattttttcg ctactcaatg tcattcagac cgtgccttcg gtggcgctct
    11221 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctgggtaagc
    11281 tcggcatatc aggaaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    11341 tgccgctggt gcgcggcgtg gtagtcggct tgaaccagat cccgcgcgat gtgctggaga
    11401 gcgccagagc gatgggcatg agcggggcgc agcgattcct gcatgttcag ttaccgctgg
    11461 cgttaccggt atttctgcgt agcctgcggg tggtgatggt gcaaaccgtc ggcatggcgg
    11521 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    11581 gcagtgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctga
    11641 tcgatgcgct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    11701 agccatgtca gcaaactgtt cggcgcacaa aaagccgtta acgatctcaa tctcaatttt
    11761 caggagggga gtttttcggt gctgattggt acatcaggct ccggtaaatc caccaccctg
    11821 aaaatgatta accgcctggt ggagcatgac agcggcgtga tccgctttgc cggagaagaa
    11881 attcgctcgc tgccagtgct ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    11941 ggcctgtttc cccactggag cgtggcgcaa aatattgcca ccgtgccgca attacaaaaa
    12001 tggtcacggg cgcggatcga cgatcgtatc gacgaattaa tggcgctact ggggctggag
    12061 ccaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    12121 gtggcgcgcg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgcg
    12181 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcataca ccgtttgctg
    12241 gggcgtacca ttgtgctggt cactcatgat attgatgagg cgctacgact ggcagaacat
    12301 ctggtgttga tggatcacgg tgaagtggtg cagcagggca atccgctgac gatgctgact
    12361 cgtccggcga atgattttgt ccgccagttt tttgggcata gtgaactggg tgtccgcctg
    12421 ctttcgttac gtagcgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggcg
    12481 ctggcagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggatgtgaa
    12541 gtgctgccgg tggtgaacac gcagggcgag ccttgcggca cgctgcattt tcaggatctg
    12601 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgcttt
    12661 gtttgtggca ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    12721 acaactgcca cgccccgttt atcagcaaga aagttttgct gctctggcac tggctcattt
    12781 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcactggtg caggaattat
    12841 tgttactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    12901 acagactttt ccgcccgtcg cggtgctggc gattgccgtt ccggtgattg ggtttggtct
    12961 gcaacccgcg attatcgcct tgatccttta tggtgtgctg cccgtcctgc aggcgacact
    13021 tgccgggctg ggagcgattg atgccagcgt gacagaagtt gcgaaaagta tgggaatgag
    13081 tcgaggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    13141 tgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggggc
    13201 cagcacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    13261 ccagggggcg ttactggtgg cactggcggc gatcattgca gaccgcctgt ttgaaaggct
    13321 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    13381 attccgccta acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    13441 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    13501 aaatgccgag tgggaaaata tcatggcctt gttcctgcca actggtgagt tgctgctgtt
    13561 gggcggaggt tcgattttca ccgcaccaca ccagcaatgt acggccttcg aacagttcag
    13621 ggcgtagttg attgagcgag tgggcgagga catcaatgcg ccatccttgt tgactggcaa
    13681 tccagccctc cagccacaga cgggtggtat cgtgaatatt ccagccaacc accagcgcat
    13741 ctttaccctg ttttttacgt gccgaagcca ggcaaatggc gatgtagttg atcagtacgc
    13801 cgtcgaggat cgccagcagc gcctggagag tcggttgttg gcactgaagt cgtcggcgca
    13861 gaggaataaa cagatgcgtg gtgagtgtct gggcggggta atcctgaccg cgctctttga
    13921 tccacgttcg caggctatgc agattgccgc tttgcaggta agtcagtaat gtttcttgct
    13981 gatcgcgcca gccgttctgc acatcaacat tttcattact gagcagcatt ttaactttgc
    14041 tgacctgcac gccgttgtcg atccagcgtt tgatctcgcg gatccggtca atatcggcat
    14101 cattgaacag ccgatgaccg ccgtctgtcc gttgcggttt cagcaatccg taacgcctct
    14161 gccacgcgcg taacgtgaca gggttaatat cacaaagcaa cgccacttca ccaattgtgt
    14221 aaagcgccat cgtctcaccc ttgctcgcga ggtcccggtt taactttaga cgcagttttg
    14281 cgaaccaggt agttttgccc gttttttgtg catctatagg gtgattttat ttttgccagg
    14341 cgattttgag tgatcgtact cacgaattct catttttctg caagagttca aagaaagtta
    14401 aacgcaggca atgtatgtta cgcgttttaa agggaagtgt ggtttgcggg tatgtacgat
    14461 tttaatctgg tgttgctgct gcttcagcag atgtgcgttt ttttagtcat tgcgtggtta
    14521 atgagtaaaa cgccattatt cataccgtta atgcaggtca cggttcgtct gccgcataaa
    14581 tttctctgct acatcgtctt ttccatcttc tgcatcatgg gcacctggtt tgggctgcat
    14641 attgacgatt ctattgccaa tacccgtgcg ataggcgcgg taatgggcgg cttactcggc
    14701 ggtccggtcg tcggtgggct ggttggtctg accggcgggt tacatcgata ttcgatgggg
    14761 ggcatgaccg cgctaagttg catgatctcg accatcgttg aagggttgct cggcggcctg
    14821 gtacacagca tcctgatccg tcgcgggcgc actgataaag tctttaaccc cattaccgcc
    14881 ggtgccgtca cgttcgtcgc tgaaatggtg caaatgttga tcatcctggc gatcgcccga
    14941 ccttatgaag atgcggtgcg tctggtgagt aatattgctg cgccaatgat ggttaccaat
    15001 accgtcggcg cggcgctgtt tatgcgtata ttgctcgata aacgcgcgat gtttgaaaaa
    15061 tacacttcgg ctttttctgc cactgcgcta aaagtggctg cctcgacgga aggcattttg
    15121 cgccaggggt ttaacgaagt gaacagcatg aaagtggcac aggtgctgta tcaggagctg
    15181 gatattggtg cagtcgcgat taccgatcga gagaaattgc tggcctttac cggaattggt
    15241 gacgaccacc atttacccgg caaaccgatt tcttcgactt acaccttaaa agcgattgaa
    15301 accggtgaag tggtctacgc tgatggcaac gaagtacctt atcgttgctc tttgcatccg
    15361 caatgcaaac tggggtcgac gctggtcatt ccgttgcgtg gcgaaaatca gcgggtgatg
    15421 ggcaccatca aattgtatga agccaaaaac cgtttattca gttcaatcaa ccgcacgttg
    15481 ggcgagggga ttgcgcaact gctttcggcg cagatcctcg ccgggcaata tgagcggcaa
    15541 aaagcgatgc tcacccagtc agagatcaaa ctgcttcacg cccaggtgaa cccccatttt
    15601 ttgtttaatg cgcttaacac cattaaagcg gtgatccgcc gcgacagcga acaggccagc
    15661 cagctggtcc agtatctttc cacttttttc cgcaaaaact taaagcggcc ttcggagttt
    15721 gttactctcg cagacgaaat tgaacatgtg aacgcttatc tgcaaattga aaaggcgcgc
    15781 ttccagtcgc ggttgcaggt caacattgct attccgcaag aattatccca gcagcaattg
    15841 cccgcgttta ccctgcaacc gatagtggaa aacgccatta aacatgggac atcacaactg
    15901 ctggatacag ggcgagtggc gatcagcgcc cgacgtgagg ggcaacattt gatgctggag
    15961 atcgaagaca atgccggttt gtatcaaccg gtaaccaatg ccagtgggct ggggatgaat
    16021 ctggtggata agcgtttacg tgaacggttt ggcgatgact atgggataag cgtcgcctgt
    16081 gagcctgatt gttacacccg aataacgtta cgactaccat ggagggacga ggcatgatta
    16141 aagtcttaat tgtcgatgat gaaccgcttg cacgggagaa cctgcgcgta tttttgcagg
    16201 agcagagcga tattgaaatc gttggagagt gttcaaacgc cgtagaaggg atcggcgcgg
    16261 tgcataaact gcgcccggat gtgctgtttc tcgatatcca gatgccgcgc atcagtggtc
    16321 tggaaatggt ggggatgctc gaccccgaac atcgaccgta tatcgttttt ctcaccgcgt
    16381 ttgacgaata cgctattaaa gcctttgaag aacatgcctt tgactatctg ctgaagccaa
    16441 ttgatgaagc gcgactggag aaaacgctgg cgcgtttgcg tcaggaacgc agtaagcagg
    16501 atgtttcgct gttaccggaa aatcaacagg cgctgaaatt tatcccttgt acggggcata
    16561 gtcggattta tttactgcaa atgaaagatg tggcatttgt cagcagtcgg atgagcggtg
    16621 tctacgttac cagccacgaa gggaaagagg gctttaccga attgacatta cgtaccctgg
    16681 aaagtcgtac accactactg cgctgccatc gtcagtatct ggttaacctc gcgcatttac
    16741 aggagattcg tctggaagat aacggccagg ccgagttgat tttgcgtaat ggcttaaccg
    16801 tgccggtcag ccgccgttat ctgaaaagct taaaagaggc gattggcctg taaaagactg
    16861 ctaaaatggc tttttgcctc atcaacacct gaaggcctca tgctaagtaa cgatattctg
    16921 cgcagcgtgc gctacatttt gaaagccaat aataatgacc tggtgcgtat tctggcgctg
    16981 ggtaatgtcg aagccaccgc ggaacagatc gccgtctggc tacgtaaaga agacgaagag
    17041 ggttttcagc gttgtccgga cattgttttg tcgtcattcc tcaatggcct gatttatgaa
    17101 aaacgcggca aggatgagtc tgctccggca ctggagccgg aacgtcgcat taataacaac
    17161 atcgtgctga aaaaattacg catcgcgttt tcgctgaaaa ccgatgacat tctggcgatc
    17221 ctcaccgaac agcagttccg cgtttcgatg ccggaaatta cagcgatgat gcgcgcaccg
    17281 gatcataaaa acttccgcga atgcggcgat caatttttac gttattttct gcgtggactg
    17341 gcagcgcgcc agcatgtgaa gaaaagctaa gacgggtatg gcggccatgc aaaaacatgg
    17401 ccgccgacag attatttcac ttctttaaaa ccagcggctt tcatcaccag ttccatttgc
    17461 gccatagtga tacctttttt ggcatcttca gcagaaacgt tgattcctga aataccctgc
    17521 agggctttga aatccacttt ttccatatcg atagtcacgt tttcctgcgc gtaagtatcg
    17581 gtataggtta atttttcttc aacacccgcg atgtttttgt atttggcgct taacggctca
    17641 agtgtcctgg cagcgtcttc tttggtggtt gcaccaatgg aggcaaattg aattttggtt
    17701 tcagaagatt gcttaagcac cttgtcacct ttgtagacat aggtaatggc aatttcagta
    17761 ccgttcagat tggcgctgaa ttttttcgat tcttctttgt caccgcagcc agcaagagag
    17821 aaaaccagaa cagatgcaac aacgagggaa aacagcttat tgaaagcctt catgtaaaac
    17881 tccattttat ttaatcaagg aactggtgac tctcaccagg ggctatatag gatatgccta
    17941 ataccgtggc gtgagcagtc cggaactgga gtagaactct tagtaaaaag cactatttca
    18001 tccttgttgc tgaagcatgg ggaataattg ttcgcacagc aaaacaccgt tattcattgc
    18061 ttctacccgt gcctcgcttt ctgtattacg aaattgtccc aacacatgtg ccagccgata
    18121 aaaacccacc gcggtgaggt cattcgccag caactctgcc tgaccaatag cactatgttc
    18181 ctgatagcgc cagccgttat ggagcagttg aataagtaac gcctggcagc gcatcagcaa
    18241 ctgatgagca gtagacggca caggcagaac gctggcagaa ggtagtgatg ccaccacagg
    18301 cgcagtttct gcgtccagcg cccaggcacg agtctttgtc atcattacct gtggttccag
    18361 tgtcaattgc ccctctacaa agctgacaaa gccagaaacc agacacacgg ggtcgtctgt
    18421 ttgttgcaaa agcgccgcca tgcgttcaac ggcataaggc gcgctggctg aggctggcag
    18481 tgataacgtc agcagattat cttcaccttc gccgctgatt acctgcgcat ccagcgtctg
    18541 gcggctgcta tcccaaccga gcgaaataca ctcagcgacc ggcagaataa ataagttatc
    18601 gacctgatta agaggccgta tgcaggcggg gggacgctgg cgtaaatatt cccgcaaagc
    18661 cacaatgccc ggctggcgta acggcgcgct caacatttgc caggcatcag gcgacagcgg
    18721 cacaacgctg cttaagcggt tgcgggtagc taacagcagc tcgccatcgg cactgcgttt
    18781 tgctgcttgt gaaacaattt gcccgcccgc cagtgcgcca gcctgaaaac taaacagccg
    18841 acgcgtcgct gccggtgagt tttcctgttc gcttctcggc caactacgcg aaagatgcaa
    18901 aatactgccg gtgtcgggat cggtaaacca gatgcgtaaa ccataatgct caatatcctg
    18961 ccagcaacgc atacctaaag acaccagccg cagatgatca agctttgctt ctccggcaat
    19021 gccagagcca acgaccgtgc gccacggcat gggcggtact tcaccgacac tgtcacgtcg
    19081 cgctatctct tgtgcactgt tcaagcgact ggttaacgcc gcaagctggc acaagcattt
    19141 tccggcatga tagtggctgg cgcgggcgtg gaaggcatca acgctggcgc gcagttgccg
    19201 tagcgattca ctcacccatc gccagttgca gcgttccgcc gcctgctgtg cgcggctgaa
    19261 agcggcctcg taatggattg gcggctggct gatgccgccc agccataatg cctgacttaa
    19321 ttgctgaaca tattgacgac acgcgttgcc ctcttcgctg gcaaacggat cgtcagatga
    19381 tgtgacgtgt tcgctgcgca tctgccagat taaatgagta aattccgctt gctgagtttt
    19441 ggcctcgacg aaggcctgca cagccagtac gacatgttcg caaagtgtgc cgtcaataca
    19501 atcacagcga gcgaaacgaa tgctgctgcg ggaataaaaa cgtacatcgc tcatcggtaa
    19561 gcgggcagag ggaatttcgc ccggcgtaca gaacaactca atggtgatgc ctttagcgac
    19621 cagcgcctgt gcgcgtttgc gggtggcatc gggcagggta gccagttctt ccagccagat
    19681 tgccggatcc cactcttctt ctttttccgt aggctgggtg gtggcgcaaa gtcgctgata
    19741 acttaaaacc agcatcacgc gatggcggca catgccgctg gccccgcagc tgcactgagc
    19801 ctctttcagt gcctggccgt tcgccagctg ggtgcggaca ccgtcactga aggtggcgat
    19861 taaagcgccg ttctcatgac tgatttccgg gacgttgcca ttttccagtt ctttaaggct
    19921 gcgcttaaca aaaccggcat tgcttaacgc cgtcagtgcc tgtggtgtca gttctaataa
    19981 ttccggacgt agtgaattca tgactgaaga ttctccgcaa gccaggtcgc cagttcgccc
    20041 ggcgtcatgg cggctatttg tgcaccgaca ttaaccagtg cctgggccat atcgcggtca
    20101 tagcaaggtg ttgcggtgct atcgagcgcc gccagtccca gcactttgat gccgctctgg
    20161 acacactttt tcacctgatg cgtcagcaat gatgatgaac caccttcata gaaatcgctc
    20221 acgaggataa tgacactttt tgcaggttgt tcaataagtt gccgaccata ctccacggca
    20281 ctggcgatat tggtcccgcc gcccagttgt actttcatta ataactctac cggatcggca
    20341 acgtctgccg tgagatcaac cacgctggtg tcaaacgcca ccagatgggt acgaatgccg
    20401 ggtaactgcc ataaacaggc cgccatcacc gctgagtgga tcaccgagtc aaccatcgat
    20461 ccgctttgat caaccagtaa gaccagttgc cattgatcgc tatggcgctt aatgcggctg
    20521 ttaaagcggg gggattcgat atacaacttg ccgtgttgcg gatgccagtg ttgcaggttg
    20581 gcacgcagcg tacttttgaa atcgaaatta cgcgccagtg gaataaatga gcgacggcgg
    20641 cgatcgcgga caccagaaaa agcctgacga acttcctttg ccagtcgcgc cataatttct
    20701 tcaacaacct ggcgcactat tcggcgggcg gcagccagca cttcgggatt catcagatgt
    20761 ttggtgtgca aaacggcgcg tagcaggctt tcagaaggct gcatacgttc cagcacgtcg
    20821 agattcgtca ccacatcttc aatgccgtag cgcagtacgg catcgctttc cagtcgctcg
    20881 atcacccgtt gcggaaacag cgtgtgaata ctgttgatcc actcaggggt ggtgagattt
    20941 gagtcaccta atccaccgga gcgttcacca cgctggagcc gttcaggatc gcgcccatac
    21001 agccactcca gcgcgtggtc tatctgccgg gcgttgtcat ccagcccaca aagcgtcgtt
    21061 tctgccgctt cgccaagaat taatcgccag cgttgtagct cacgggtggt cagaagatcg
    21121 ttcagttcag acatggaaaa ctccccagtg ttgcagcgat gccagtgcct gctgttcgag
    21181 ttgttgatga tgtgcaatgt cttgtggtgg acaatgcaac ggcatttgca gtgccgaaac
    21241 gggaagttgc accagttgat aatgctcaag cacctgatgc gccagcgtcc cgcgttctcg
    21301 tggtggtagc caggccatcg ccgcgcgtaa atcgggcagg gcattgataa aatcggcatc
    21361 actcagttga tttaaatgac tgctgaaacc ggcgataaat accggctgac aggccagttg
    21421 atgacgggct aatgccagca aaccgtgcag cgcctcgccg gattgtgtcg gggataattg
    21481 caccagcatt gtcagcgccg cttcggcact ggcgttggga tgttccagac gcatcagtgc
    21541 gccaagtgcc gcgccgcgat ccagcgccgg ggcatgaatc gcctgactgc gtcgctccag
    21601 taaggctacc gccgcagaaa gagaaacgcc gggtaaatta acgccgctat gtagatcgcg
    21661 caggatatgg caaagcgctt gccagctatg caggtgagcg tgaaactcct tttcatccgg
    21721 tctgccgttg gattcacata gccacagcgt gcgatcgatg gccgcgcata atgtcgtttg
    21781 taatatctgc gcgccctgca taccgctaat ttcatccagc cgccataagg catatagcac
    21841 ttccagcgcc tggcccattt cggcaaactg attttcctgg gcgattaata atgtgagttg
    21901 ctccagtaat tgttgattga aggacgccag acccgctaac gccgcctggc taagacatgt
    21961 ggtgatactg ccgattccac ccgcgtccag catatcggct tctaatttat tgcgtgcggc
    22021 ttcctggagt gtggcaccaa agcaggcggc ctcaattaat gccgcatgtt ggctaagcgg
    22081 gcgggttaat ttccagcgtt cttcaccgtt gcctgcaagt gtcagtgtac ttccctgctg
    22141 gcgcacaacc cctgggatct ccagtattac cagccgatgt aacacctgac tttgcgccag
    22201 tccatcgggg gtaaagcgat ttaaagtaag ctccgccgga aaagagattc ctgtacgttc
    22261 cagttcgcag gtgacatctt ttggcagagg cggctgtggc gtagaggggg caagtttacc
    22321 gaatccgtca cccgctaatg tgtctatcaa cgttagcaga atcggatcgg tatcgggatg
    22381 aataacgccg cgatagctcc acggcaacgg cgcattcagg gcttctttaa tcagcgagcc
    22441 tgctatggca tccagccagt cagtgcgtaa cggtagtgta tgaccacgca actgtgccag
    22501 tgccatcgca tgcagatgag cggcagccat atccgctgtt gaagcaggca agtggtgctg
    22561 gcgcaaacgg gtaagaaccg ttttcagcag ttgttcacct gcctgctgca agccccaccg
    22621 ccagcaccag ttttgccaga ccggggcagg cataccggaa aggtatcctg ccagcacatc
    22681 aaggcgcttt tcactgtagg gggtgagata acaacctgta acggcatctg ccagcgaggg
    22741 caattccggc gtgttaattt cctgcgggca ttcgcgccac atttttgcca gtgcagaagc
    22801 gtgccagcca ccgcagacga ctaacacgtc gccattgttt tgctgcatcg cccaaccaat
    22861 ccagcgggcc ataaagtctt cgcgctgacg attgagagca tctccggggg aatcgccccg
    22921 cagttgtgca aaatagtgcg ccagtgcaga gggtaatgca gtttgctggc tttcatcttc
    22981 gaacaagtga tcccacaggg tgtcgctgtt atccatgcgg gtggcacgca gcagcaaggt
    23041 ctggctttcc tcttgcgtat caggcgaatc gtcctcttct tcactctgcg cccagcaagg
    23101 caaatcgatg aagtaagttt gtgcctgaat acgacgagcg gcttgtagcg cctgccactc
    23161 cggcgaaaat tcagcatatg gcgtccaggc accacgcccg ggggctgcac cgtcctgata
    23221 ctggcaataa ctgtaaatag ctaccggaag ctggtgggct aaaaacagtt cgtctacccg
    23281 gtcattaaaa tcagccgggc cttcaatcaa cacgtatcgt ggccgctggc tttcgattaa
    23341 cgatttcacc agccgggcgc aggccggact atgatgccgg atgccgacaa ttaacggctc
    23401 gctcatgcat ctttcctcac ggcaggcggt ggcgagcttg ataataagcc tgccagtgag
    23461 cttctttatg tgtggcgact cgctgttcga agtaacggcg cagacgagcg cgatcttctt
    23521 cgttatcttt gacgatggtt ccggcaatac agtccaccag atcggctggc tcacctgcgc
    23581 gattcgctaa aaaccaggcg cggacgccca ctgcgtgggc cacgttcacg gcttcagcgg
    23641 tggacataat tgccgtcagg gtatccattg acgttttctt ttcgccgttg gcacgcagat
    23701 cgcggaaggt gcggaccagt aactccagta ccgcgtcagg cactttatgc ggaataccgc
    23761 tatgggccag caaacgcgcc gaagcactgg cgaccagttc cagctcctgg gcgaaatcca
    23821 taatcggaaa gacggtttca aaatcgaagc gacgctttag cgcggcactc atctcattga
    23881 cgccgcgatc gcgggtattg gcagtggcaa taatattaaa gccttctcgc gcatagagct
    23941 gactggcttc accggtgagt tccggcaccg tcattaccct gtcagaaagc atacctaaca
    24001 gacagtcttg tacttccagc ggtgtacgcg ttatctcttc aaaacggaca attttgccgt
    24061 cgcgcattcc ctggtaaagc ggggcaggga ccagcgcttc cgttgacgga ccatggttga
    24121 taagcagggc gtagttccag ccgtacttga tttgatcttc ggtggtagat gcgccgccct
    24181 ggatggttaa cccggcatcg ccgctaatag cggttgccag taattcagac aggagcgatt
    24241 ttgcggtgcc aggttccccc accaacatca aaccgcgccc ggtagcgaga gtgactaaca
    24301 tgcgttcgac agcggaggga ctgatcacga ctttacggct aatattttgc gcgctatcgc
    24361 caaggataaa agcgcgggcc gcaggcaaac ttaactgcca accgggcggg caaggtgcgt
    24421 gatcattttg ttttaatttt gccagttcat cggcgtataa cacagcggca ggcggacgct
    24481 gcagatgatt attctgcggt gacatatatt catccctgaa atgtattaat gaaataaacg
    24541 atattttacg cgcaataaat gtttgccgca ttagttgttt gagcgaaaaa tatctggaaa
    24601 gcagatcaca gtaaataaaa aaacctgctg gtgaggcagg ttcttttatt tactacaatg
    24661 acgctaactt gctgattgtg caacatttcg taccaacgaa taaattataa ccatatccac
    24721 actccattgc aattcagtga atgagattta taatcctgcc cgttgggagg ttgtgccgga
    24781 tcggaccaat tttaagtcct ggctgctgga cgggtggtgc cgcaggcggt gccctgatcc
    24841 ggagagcaac gtttcgtacc aacatacgta aaccagtatc gacaggcagt gtaagggtaa
    24901 ggtgctggtg ttgcgctcct taataaggag agcggatgga cacgctgaca caaaagttaa
    24961 ccgtgctcat tgctgtactg gagctattag tggctctgtt acggttgatt gatttgttga
    25021 agtaacgggc aaattgaaac gcatctaagg gggaataccg tttccccctt taataaaatg
    25081 attactgttt ttattaattc atcatataac tcatttttat ttataaattc atcgatttta
    25141 taatcaacgt actatttggc cgggatgtga atatttatgg tgggctattg gcgaaaaata
    25201 tctggaaagt agatcgcaga aaataaaaga acctgctggt gaggcaggtt cttttattta
    25261 ctactttgat gttaagttac tgatagtgca acatggcatc tcaacgaacg aattataacc
    25321 accgctggac cgtgttgcaa ttaagataat gagatttata atcctgactg ttgggaagtt
    25381 gcgccggatc gaacccattt caagtcctgg ctaccggacg ggtggtgccg caggtagtgc
    25441 tctgatccgg agtgcaacat ggcgactcaa caaacgtaaa ccagtgtcga caggcagtgt
    25501 aagggtaagg tgctggtgtt g
//
LOCUS       DAZGTJ010000080        24655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000080 DAZGTJ010000000
VERSION     DAZGTJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.080"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            193..963
                     /gene="yjjP"
                     /locus_tag="ACHK8E_003747"
     CDS             193..963
                     /gene="yjjP"
                     /locus_tag="ACHK8E_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP0345961.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            954..1427
                     /gene="yjjB"
                     /locus_tag="ACHK8E_003748"
     CDS             954..1427
                     /gene="yjjB"
                     /locus_tag="ACHK8E_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP0345962.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1535..2074
                     /gene="dnaT"
                     /locus_tag="ACHK8E_003749"
     CDS             1535..2074
                     /gene="dnaT"
                     /locus_tag="ACHK8E_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP0345963.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2077..2814
                     /gene="dnaC"
                     /locus_tag="ACHK8E_003750"
     CDS             2077..2814
                     /gene="dnaC"
                     /locus_tag="ACHK8E_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP0345964.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2863..3357
                     /gene="yjjA"
                     /locus_tag="ACHK8E_003751"
     CDS             2863..3357
                     /gene="yjjA"
                     /locus_tag="ACHK8E_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP0345965.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3611..5902
                     /gene="opgB"
                     /locus_tag="ACHK8E_003752"
     CDS             3611..5902
                     /gene="opgB"
                     /locus_tag="ACHK8E_003752"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP0345966.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6040..7062)
                     /gene="lgoD"
                     /locus_tag="ACHK8E_003753"
     CDS             complement(6040..7062)
                     /gene="lgoD"
                     /locus_tag="ACHK8E_003753"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP0345967.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7201..8115
                     /gene="lgoR"
                     /locus_tag="ACHK8E_003754"
     CDS             7201..8115
                     /gene="lgoR"
                     /locus_tag="ACHK8E_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP0345968.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8330..9691
                     /gene="lgoT"
                     /locus_tag="ACHK8E_003755"
     CDS             8330..9691
                     /gene="lgoT"
                     /locus_tag="ACHK8E_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0345969.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9740..11404)
                     /gene="tsr"
                     /locus_tag="ACHK8E_003756"
     CDS             complement(9740..11404)
                     /gene="tsr"
                     /locus_tag="ACHK8E_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP0345970.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11586..12449
                     /locus_tag="ACHK8E_003757"
     CDS             11586..12449
                     /locus_tag="ACHK8E_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0345971.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12459..13928)
                     /locus_tag="ACHK8E_003758"
     CDS             complement(12459..13928)
                     /locus_tag="ACHK8E_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFP0345972.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14031..14420)
                     /locus_tag="ACHK8E_003759"
     CDS             complement(14031..14420)
                     /locus_tag="ACHK8E_003759"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0345973.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14430..15872)
                     /locus_tag="ACHK8E_003760"
     CDS             complement(14430..15872)
                     /locus_tag="ACHK8E_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0345974.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            16073..17293
                     /locus_tag="ACHK8E_003761"
     CDS             16073..17293
                     /locus_tag="ACHK8E_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFP0345975.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            17561..19711
                     /gene="btsT"
                     /locus_tag="ACHK8E_003762"
     CDS             17561..19711
                     /gene="btsT"
                     /locus_tag="ACHK8E_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP0345976.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            19761..19964
                     /gene="yjiX"
                     /locus_tag="ACHK8E_003763"
     CDS             19761..19964
                     /gene="yjiX"
                     /locus_tag="ACHK8E_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0345977.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            19975..20931
                     /gene="yjiA"
                     /locus_tag="ACHK8E_003764"
     CDS             19975..20931
                     /gene="yjiA"
                     /locus_tag="ACHK8E_003764"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0345978.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(20965..21267)
                     /locus_tag="ACHK8E_003765"
     CDS             complement(20965..21267)
                     /locus_tag="ACHK8E_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFP0345979.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(21282..21383)
                     /locus_tag="ACHK8E_003766"
                     /pseudo
     CDS             complement(21282..21383)
                     /locus_tag="ACHK8E_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            21580..22746
                     /locus_tag="ACHK8E_003767"
     CDS             21580..22746
                     /locus_tag="ACHK8E_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0345980.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            22810..24429
                     /locus_tag="ACHK8E_003768"
     CDS             22810..24429
                     /locus_tag="ACHK8E_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0345981.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
     gene            24419..>24655
                     /locus_tag="ACHK8E_003769"
     CDS             24419..>24655
                     /locus_tag="ACHK8E_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0345982.1"
                     /translation="MGSKWIEVSLGEISEKIGDGIHGTPTYNNSGNYYFINGSNLIDN
                     SIKITETTKCVDHDEYLKHRKKLSNNTVLVSINGT"
BASE COUNT         6056 a         6489 c         6167 g         5943 t
ORIGIN      
        1 gtgataatat tcatgcgata atttgtttgc gacgagagat aattcgcttt ttaacaccga
       61 gcttctcccg ataaccagga ttaatcagcg caattttgat cttaacctgc cgcaaaacaa
      121 ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac
      181 aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt
      241 ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga
      301 ctgggtcggg cgttgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg
      361 ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc
      421 attaatatgc atgtggtgac tgaagtccag cacattgtga ttctggcgga gcaccatctg
      481 ctggattaca aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga
      541 tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt
      601 ggctgggatg gtgccgtcat caccttcttt gccagtacgg ccgcgatgta tatccgccag
      661 ctgctggcac aacgtcatct tcatccacag atcaactttt gcctcaccgc tttcgccgcc
      721 accaccattt ccggtttgct tttgcaactt cccactttca gcaatacccc caccatcgcg
      781 atggccgcca gcgttctgct gctggtgccg ggctttccgt tgattaatgc cgtcgccgat
      841 atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca
      901 ctggctacct gcgtcggcgt ggtgatggca ctgacgattt gggggctacg cggatgggtg
      961 tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg
     1021 gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ttgcttggct
     1081 cgataggtca tggttcccga atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa
     1141 cttttatggc ttctatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg
     1201 cgcatccgaa agtgtttacc gtggcggctg ttattcctat gttccctggc atatcggctt
     1261 ataccgcaat gatttcggcg gtaaaaatca gccagctagg ttacagcgaa ccgttgatga
     1321 ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc
     1381 tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcactt
     1441 aaagcacgcg gatgagagac agcctcctct cctccgtgtg ttactataaa agttatctcc
     1501 cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt tgaccccgga
     1561 cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa aagctgaagg
     1621 cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtta cgcctgcacg
     1681 cctggctgaa ctgctggcgc tggaagaaaa gctggcgcgt ccgggaagcg atgtcgctct
     1741 ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca tggggaaatt
     1801 cgccatgtat ccggactggc aacccgatgc cgactttatc cgcctggcgg cgctatgggg
     1861 cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg cctactggca
     1921 ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc gcagcctgca
     1981 aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg tcagcgaacc
     2041 tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg cgacctgatg
     2101 caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cgttcaaaac gggtgaagaa
     2161 ctactggcct ggcagaaaga acaaggagcg atccgctccg ccgctctcga acgtgaaaat
     2221 cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact gcatcagaac
     2281 tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt aagcaaagcg
     2341 cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc tggcaagccc
     2401 ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct acgcggtaaa
     2461 tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac cttcaggaat
     2521 agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct gctggtgatc
     2581 gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa ccagatcgtc
     2641 gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa tatggaagag
     2701 atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa cagtttgtgg
     2761 gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta ttaatccccg
     2821 tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa aactgttaaa
     2881 catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt gcatgctgcg
     2941 tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa cagcacgaca
     3001 caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg aaaccaggcc
     3061 ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc gaagcaaaag
     3121 ctggcttcgg taaccgacgc cgaaaacatc aagaatcagg tactggaaaa actgggcctg
     3181 aacagtgaag agcaaaaaga agacaccaac tatctggatg gcattcaggg tttgctgaaa
     3241 accaaagatg gtcagcaact caatctggat aatattggaa cgactccgct ggcagaaaag
     3301 gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat ttcctgaacc
     3361 gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc ccttttccct
     3421 gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt
     3481 ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc aatatggcga
     3541 cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc ggtttagtga
     3601 ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg tgctgattta
     3661 cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acattaacgg tactggggct
     3721 atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg atggtattaa
     3781 cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggtgtca gtaaatacat
     3841 cctgcctggt atcggcattg tgctggggct gacagcggtg ttcggtgcgc tgggctggat
     3901 cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg cactcttact
     3961 ggcgttgggt tcagtggatg ccagcccggc atttcgtcag ataacggaac tggtgaaatc
     4021 ccagtcacgc gacggcgacc cggactttgc ggcttattat aaagagccgt cgaaaactat
     4081 ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt
     4141 tgataacgag gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct
     4201 ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg gcatggtggc
     4261 ttctcagtgc ggaataccgc tgtttgcccc ctttgaaggc aacgcctccg cttctgtttc
     4321 cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa
     4381 ctatttcgtg cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc tgaagtcgca
     4441 tggcttcgac cacttatatg gctcagaaga gctgaaaagc gtggtggccg acccgcacta
     4501 tcgcaacgac tggggattct acgacgatac cgttctcgac gaagcgtgga aaaagtttga
     4561 agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca
     4621 cccggatggt tttatctcgc gtacctgtaa ccgcaaaaaa tatgattttg acggtaagcc
     4681 gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt ttatcaacaa
     4741 aatcaaagcg tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg accatttagc
     4801 gatgaacaac acggcgtgga aataccttaa taaacaggat cgcaataacc tgttttttgt
     4861 catccgtggc gacaagccgc agcaagagac gctggcggtg aagcgtaaca cgatggataa
     4921 cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg gtcgtagcag
     4981 tttatccggg cagtcaatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg
     5041 gaagccggat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga
     5101 ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc tactgttgcg
     5161 ggtttcagac aaacgcgtgg aaccgctgcc ggaaagtgaa tactcagcac cgctgcgttt
     5221 ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt gctacaagat
     5281 ggcacaactt tgggctccgg aactggcact ctccaccgac tggtgtgtct cgcaagggca
     5341 gcttggcggt cagcaaattg tccaacatgt tgacaaaaca acatggaagg gcaaaacggc
     5401 atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata cgctgaagat
     5461 tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg ccggtgcgcc
     5521 agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc gctggtccaa
     5581 cgctcagctg ggcgacgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga
     5641 cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt
     5701 aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca cgctgcattt
     5761 cgataaccca accgatgccg acacgctggt aattgtgccg ccggaacctg tctcaaccaa
     5821 cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg tggaaattaa
     5881 agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcataag gtgcgaccta
     5941 cacgccttct ccggcttaca acacactatt aagaaacaaa aaaaacagcg cccaacactt
     6001 ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt
     6061 aatcaactca cgattgttaa tcacatcacg ctcgtaggtt tctgccagcg tggcgaacgg
     6121 gtagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca gtcgaccgac
     6181 tttagcgaaa tcttccggcg tggcgttgcg gctacccatc atcgtcgttt cttttttatg
     6241 gaattctgga tcggagaact gcaactcacc tttaaacaga ccgacaaata ccaccgtgcc
     6301 gccgtgacga atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat
     6361 cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc
     6421 tgatgggtcc agcacaggca actccagacg cgttgccaca tgttcacggc gcgccggact
     6481 ggtatccgcc accgctacca gtgcgccatc ggctttagcg attgccgccg cgcccagacc
     6541 gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac
     6601 cgcatgagcg ctaatagcga aaggttcaat caatgctgcc gcctgcgggt caataccgtc
     6661 tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg
     6721 cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca
     6781 ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa gatcagcaat
     6841 atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaacgc gtggataact
     6901 aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc cgacagactt
     6961 aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa
     7021 ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt
     7081 ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt
     7141 gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat
     7201 atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg
     7261 cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc
     7321 agccgcacta cagtgcgtca tattctcagc cacttacgcg aatgcggcgt cttaacgcag
     7381 gttggaaacg actatgtgat tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact
     7441 accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gatgataaac
     7501 cagcgccagt tacgcccagg ggaaactttt tctgaactgc aactggctcg ggccgcgggt
     7561 gtcagcccgg ttgtagtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa
     7621 agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc tgagcagctg
     7681 tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat
     7741 catgatcccc gctggctgca ggcgaaaacc atgctcgaac gtcatcggtt actgcgtgac
     7801 aacatcggca atagctttcg catgttctcg caacttgaca gagattttca ctccttactg
     7861 ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt
     7921 cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat
     7981 gagcatatga ccattctcag tgctctgatt tgccgcagtg accttgatgc cacccttgcg
     8041 ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg
     8101 cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat
     8161 caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat
     8221 gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg
     8281 taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga
     8341 aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt
     8401 gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat
     8461 gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggttgc
     8521 aaatttaacg attcgtgaag aattgggatt aagtgctacc gaaatcggcg ctttgctctc
     8581 cgtgttttca ctcgcttacg ggattgcgca acttccttgc ggcccactat tggatcgtaa
     8641 aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc aggccatgtc
     8701 tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga
     8761 agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg
     8821 cggacgcccg atgggcttct tcaacgcagc ttctactatt ggcgttgccg taagcccacc
     8881 gattctggcg gcgatgatgc tggtgatggg ctggcgtggg atgtttatta ccattggtgt
     8941 gctggggatt tttctcgcca ttggctggta tatgctctat cgcaaccgcg agcacgtaga
     9001 actgactgcc gttgaacaag cttacctcaa tgctggtagc gtcaatgccc gccgagatcc
     9061 gctcagtttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgat
     9121 cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc ctggttacct
     9181 gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta tccctttcct
     9241 gtttggggct gccgggatgc tggtcaacgg ttacgttacc gactggctgg tcaaaggggg
     9301 aatggctccg attaaaagcc gtaagatctg cattattgcc gggatgttct gttctgccgc
     9361 ctttacgctg gtcgtaccgc aagcgacaac atccatgaca gcggttctgc tgattggcat
     9421 ggcactgttt tgtattcact ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt
     9481 tgcttctcgc atgacggcgt cggtgggcag tatccagaac tttgccagct tcatctgcgc
     9541 ctctttcgcg ccgatcatta ctggttttat tgttgatacc acccactcat tccgtctggc
     9601 actaatcatc tgcggttgcg tcaccgcagc gggggcactg gcgtacatct tcctggttcg
     9661 tcagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac attttgctta
     9721 tcgggcattt ttatggcgat taaaacgttt cccaattctc gccgctatct gccacggcca
     9781 ttttacgcgg cgtagctggc gtcacggttt ttactacagc cgatgtttca cgctgctgtt
     9841 gctgctgttg ctgaatccgg aacactgcca ctgcttcggt caggcgactg gcctgctctt
     9901 ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttttgt tgggttaccc
     9961 ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag
    10021 cagaagcaat ttcgcccata atgtccgtca cgcgggtcac ggcactgaca atctccgcca
    10081 ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt
    10141 caatcaggct tttaatttca cgagcagcct gagcgctacg ctgggccaga ttacgcactt
    10201 ctcccgcaac caccgcaaaa ccgcgccctt gctcacccgc gcgcgctgct tcaaccgccg
    10261 cgttcaaagc cagaatattg gtctggaagg caataccgtc aattacgctg ataatatcgg
    10321 cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta tccaccactt
    10381 tgccgccgcg ttgcgccgtt tcggaagcac ttaacgccag gtggctggcc tgacgcgcat
    10441 tttcggcgtt ctgcttcacc gttgcggtca gttgctccat gctggctgcc gtctcttcca
    10501 gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg gcgatttcgc
    10561 tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct
    10621 ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct
    10681 caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca
    10741 ttggcgcgac cagcgaggct ttaataccga accagacggc gaggatgacc gccagtacga
    10801 cgatcatcac gcctaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga
    10861 cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac
    10921 cgtcctgata tccctgggtc ggttgatcaa agaactcgtt gattttgcct gcacctaaca
    10981 gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgacg
    11041 ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt
    11101 ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac
    11161 caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct
    11221 gcaacaacgc aacccagctg ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa
    11281 cagtgaaatt ttctttgtca ttctttaagg cattaaagaa cagaccgcct gatgtcagtt
    11341 gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta
    11401 acatggtttc tctttccact agacagatac actggttgtc ggcctggtgg tggaaaactt
    11461 tatgctgtgg aaaagcgtcg gcactgcttt tgttgcagca aacttgcgtt ttttgtgagg
    11521 gtcacactct atagcggcag tttatggtac aaagtatcaa tcttcttcga accagtgata
    11581 ctcctatggc ttatagcatc gatatcatct cctgcattac cgaacggttt atggcgttaa
    11641 cagccactga aaagcgtatc gcacaattta ttcttgatga cgtctctgcc gcagtagagt
    11701 tgccaattgc tgagctggca cggttaacgc aaaccagtca ggcgtcggtg acgcgttttg
    11761 cccgtgcact ggggtgcaaa gatgtccgcg agttgaaagt caaactggcg cagtcactgg
    11821 ctgtcgggca gcggtttatt ctcgatgtac cggatcttga cggtgttcag gggatctacg
    11881 aagcgattat tagcgtgctg gagacgcatc gtcgttcgct ggatatgaat gccatcagcc
    11941 aggctgtaag ctggttaagt caggcgcggc aaattcttgc gttaggaact ggcggcggtt
    12001 ccactatctg ttcacaggag attcagtacc gtttattccg tctggggcta ccggtggtca
    12061 gtcagaatga tgccctgatg atgagaatga tgtgctcggc agtcatgccg caggatgtgg
    12121 tgattgtgct ttcgctgggc ggttatacac cggaaatcat cgaaagtgcg gcaatcgccc
    12181 gtcagtatgg cgcgcgtgtt attgccatta cgccagcaca aacaccgctg gcggaacagg
    12241 tggatctggt attgccgtta ctggttcagg aaagtgatta catctttaag cccacccctt
    12301 cccgctacgc gatgttagcc atggtcgatg tactggcaac agaactggca atggcaaata
    12361 aagcgcaggc gaaagatcgc ctgcggcgga taaaactcgt gttagacagc caccgtgggg
    12421 gcgctaaccg ccagccactt ggcgattagc attgtcggtt atgcttcttc aggtttggtg
    12481 atcacgacac tctggttttc acgctcattg gtgataatag ccaaagcttc ttcccggctg
    12541 acctgacttg ctggcgttac caacgtcacc agtacgccag cgaccaggct gcccagcact
    12601 gacggaatac acgggttacc ccagtaagcc agccagtcgg cattcatcag caccacaatt
    12661 gacatcacca taccgctcac taacgccgcc agcgcgccct gccagttaaa gcgtagccag
    12721 aagcgaccga gaatcgagca gataaacagc ccggacatca gcatcgaaat cattttggta
    12781 atgtagctga tgatgtcatt agaggtcaga gcaaagacca gcgccaggcc aataacaaag
    12841 gtcaacatcc agcgtgagta agtaatggcc ttgtcctccg gcggcattct gccggtaatc
    12901 atggtgtaga cgtcgcgcat cataatcgcc accgccgcga tggcatcgga gctggcagaa
    12961 gacatggtgg ctgaaagccc ggcaatcagc accaccagcc caagaatggc aggtaagaaa
    13021 ctggtcgcga acaagaaggc gtagttgctg ttttccagat ttgggttcat ggtgaacgcc
    13081 gccatgccga taatggcagg caaaatcgag aagaacagat ataacacccc agtaataaca
    13141 aacgaacggc gtacagaggg aacatcttta cccgagtaaa tacgctggcg ataagacggt
    13201 gttgccagca cacctacgcc aataaccagt gccagcgaaa gcgcaggcag cacgcccatt
    13261 ttatcaatcg caaacaggct catcgctttt gggtccatcg cctgctcaat cgcactccag
    13321 ccgccaacgt gaacaacggc aagaatagcc attaatataa agccaaagaa cagaattagg
    13381 gcctgaatag tatctgtcca cactaccgcc gaataaccgc cgataataac ataaatcgca
    13441 aaagctaagg cgataattaa ttttgccaca gtaagatcaa tacccgtcgc ccaggcaaga
    13501 tacatacttc cacctaatat atgcgccccc agccagccaa tagaggcaat aaatatcatc
    13561 aggccgacaa tatttttaat taaatgacta ccgccagtat aataagagag ttcttcactc
    13621 atggtcataa aacgtaattt acgtaccggt gcaaacagcc acgccaccag caatatacct
    13681 acggctccac cgacgccata aagcattccg gcccagccat tgctgtagcc aaagcccact
    13741 gcccccatac tggaacccgt gccaaccatc gtggcaacgg ttgaccccag cgtcagaaac
    13801 agcggcagcg aacgcccgcc cagcagaaaa tcttctccgc ttttctgatt acgagaaaca
    13861 aaccaaccaa gccaaatcat ggcgatggca taaataataa agccgactaa aaagatatga
    13921 ctgttcataa ttcacatcct gccagtagag agaaattaat gtaagagttc ccggagtgct
    13981 gaatgtattc agcactcaat tgcggtctgt ttttttaatt cttaaattta ttagcgggtc
    14041 gcgttataac aggtcacatc gacttcgact ttacaatcga caaccagatc agccacacaa
    14101 caaatacgtg caggcggatt ctcgccaaaa aattctctga atactttatt aaatgactgg
    14161 aaatagcggg cgtcggtcag gatcactttc acgtgaacca catccgccag gctataaccg
    14221 gcttcagtca tgatgtcgac acagttttgg atcgccagtc gtgactgttc aacgattccg
    14281 ccttctacca cttcaccgtt tttcatgggt gtttgcccgg agacgtacag ccagccgccc
    14341 gcctcaaccg ccctggcaaa aggcaaatgc tgcccgccag tgcctgttcc accttccgta
    14401 ccgtaacgtt taatgctcat attcactcct taatgtgagg tctgccggcg caggaagcgt
    14461 cccgcacggc ctgtaatatg tttatcggga ccatagctca taacgccgtt caccatcaca
    14521 gcctcaatgc catctgccgg gcgtttggga tcagaaaagc tggcaacatc acgaatggtt
    14581 tgtggatcga acaacaccag gtccgcaaaa taaccgactt tcaccagtcc acgctggggc
    14641 aggcgaaaac ggctggcaga aagcccggtc attttgtgga tagccgtggt cagtgggaat
    14701 aagccttcat cacggcaata gtggcccagc acgcgaggga aggcgcccca taaacgtgga
    14761 tgcggcatgg gatcattggg caggccgtca gagccaatca tactgaccgg ataactcaac
    14821 actcggcgga cgtcgcgctc gtccatgttg taatagatag ccccggcagg cattagcagc
    14881 ttcgcggcct ccagcatact catctgccag tcgatggcga tttgcgccag tgttttcccg
    14941 gcctgctgcg gatgtgtttg tgaccaggtg atcacaatat cgaattcgtc ggtgacttgt
    15001 ttgagatcca gcgttgaaga gctggctgag taagggtaga catcgcagcc aatttcctgg
    15061 tgttgacgca tttggtcaaa aaaggccaac gtttcttttg tccgccccca gtttttcgcc
    15121 ccggcgcatt tatggtggga aaccacgaca ggcactttgc catgacgccc aatacgaaac
    15181 gcctcatcca gggcatcgag aatcggttca aattcagaac gcagatgggt ggtataaacg
    15241 cccttctcac cggctaattc ttccgccagc gccatgactt cttcggtagt cgcctgaaat
    15301 gccgttgcat aggccagccc ggaacttaac cccagcgccc cctgactcaa cgccagacgt
    15361 aagtcggcac gcatagcggc aatttcatcc accgtcgccg ggcgaaacag gtcgtccata
    15421 tggttattac gcagcgccgt atgaccaatc agcgtgccaa cgttcagtga cggcctggct
    15481 gcttccactg cctgggcata gctttcaacg gtaggataaa taaagtgctc tgcttccccc
    15541 aacagattca tcgggtcagg aacttcacct ttcattttcg ccgacgccgc actaatcccg
    15601 cagttaccga caatcaccgt agtgatcccc tggctgatct ttggcagata ttccgggatg
    15661 cgaataacgt tgatatcgtc atgggtatga acgtcgataa acccgggtgc cagcacccgc
    15721 ccttccccgt caatgacctg ctgcgccgta acgttaagcg caggggcaat atcgacaatc
    15781 cgatcacctg ttattgccac gtcaccgcga aattcagggc cgccgctacc gtcgataacg
    15841 gtcacatttt tgattaacca gtcaacctgc atgttcgtca ctccatttct gcaatggtga
    15901 caagttaacc atttacaagg acgaaaaaca gtggaaaact acgcaaaacc cagatgattt
    15961 tatgatactt ttattcataa ataataagat aaataaacta aatatttata tttcatttag
    16021 ttacaaatga tactcacatc cgatacggtg aaaaacttaa aaggtaaatg ttatgaaata
    16081 ccactcagaa cctctggttc cccataaatc tgccctgatg caaatgcccg caaatctcct
    16141 tgccgaagat gtctgtttac ctgccgcgat cattaaaaag caggccctgg agaacaacat
    16201 tacgtggatg caacgctacg ctgacgcgcg cggcgtttca ctggcaccgc acggtaaaac
    16261 caccatgacg ccgtggattt ttcaggcgca gcaggcggct ggtgcctggg caataggtgt
    16321 cggtagcgca tggcaggctg gtgcggcaat ggcgagcggt attcagcgag tgctgatggt
    16381 taaccagctg gtcggcaagg cgaatatgca gttgattgcc cagttgcaac gacattaccc
    16441 gacggtcgat tttatctgct gtatcgacag cattgagaac gcccgcgctt tgtctgcctt
    16501 ttttgccagt cagcaacaga cactgaacgt gatgattgag ttgggtgtcc ccggcgggcg
    16561 ctgtggttgc cgctcaaggg atgccgcact gacactggct aaacaggcag cccaattacc
    16621 ggggttacgc ctgcgggggc ttgagttata tgaaggcgtt ttacacggtg acgatccaca
    16681 accgcaggta gaagctctgc tgcgggatgc cgcacaactg gcctgtgata tggcaagtct
    16741 ggttgatggc gagtttattc tcaccggagc aggcacggtc tggtatgacg tggtgtgcaa
    16801 tatctggctg gcggcagaaa agcctgataa ctgccgcatt gttattcgcc ctggctgcta
    16861 catcactcac gacaccggga tctacgacgc ggcacaacaa cagttgcttg cccgtgatcc
    16921 tgtggcctgc gatctggcag gcgatctcac atctgcgctg gaactggtcg ccatggtgca
    16981 atcggtgccg gaagctgatc gtgcggtggt aaattttggt aagcgtgatt gcgccttcga
    17041 cgccggactg ccgcaaccag tcgcccacta ccgcaacggc aaatcactgg catttgatcc
    17101 acaggcgatt cgcagtacag gtattatgga tcagcactgc atgttgcaac tgtcagctaa
    17161 tagcgacgtg caagtggggg atattctggt gtttggcaca tcgcatccgt gcctgacctt
    17221 cgacaaatgg aaaacgttgt tattgactga tgacgactac aacgtactgg cagaattaga
    17281 cactctcttc taagaacgcc ccttcggtta attatcgaag gggcattttt attgtgatct
    17341 caatcacata tccacccgac gaataattct tgcccaaccg ctcaccgcca ttcctgaccg
    17401 cttacatccc taaaataacc actcagttat ttaccttact ttacgcgcgc gtaactctgg
    17461 caacatcact gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa acctagaacg
    17521 gcttcggcca actattaatc aatacatgcc aggttttact atggatacta aaaagatatt
    17581 caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg cggtagttgc
    17641 attacgtcgg ggggagcacg tcagtgccct gtggatcgtg gtcgcctctg tgtcggtgta
    17701 tctggtggct tatcgctact acagtctgta catcgcccag aaggtgatga aactcgaccc
    17761 cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactatgttc cgaccaaccg
    17821 ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc tggttggtcc
    17881 ggttctcgcc gcgcagatgg gctacctgcc tggcacgctg tggctgctgg cgggggtagt
    17941 gctggccggt gcggtacagg actttatggt gctgtttatc tcctctcgcc gtaacggcgc
    18001 atctctcggt gagatgatca aagaagagat gggaccagta ccggggacta tcgcgctgtt
    18061 tggctgtttc ttaatcatga tcatcatcct tgccgtcctg gcgctgattg tggttaaagc
    18121 cctggccgaa agtccgtggg gtgtcttcac cgtttgctca accgtaccaa ttgcgctgtt
    18181 tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct ctgtcattgg
    18241 tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg atccgtactg
    18301 gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg gctatgcgtt
    18361 tgtttccgcg ctgctgccgg tgtggctgat cctcgcacca cgcgactatc tggcaacctt
    18421 cctgaaaatc ggcgttatcg tcggcctggc gctgggtatc gtggtgctga acccggaact
    18481 gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga aaggcgctct
    18541 gttcccgttc ctgttcatca ccatcgcctg tggtgcggta tctggcttcc acgcgctgat
    18601 ctcttccggt acgacgccga aactgctggc taacgaaacc gacgcgcgtt tcattggcta
    18661 cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgctg cgtccatcat
    18721 cgaaccgggt ctttacttcg cgatgaacac cccgcctgct ggccttggca tcaccatgcc
    18781 taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagt tgaaagacgt
    18841 taccgcacac gcggcggcga ccgtcagctc ctggggcttc gtgatttcgc cagagcagat
    18901 cctgcaaacc gcgaaagaca ttggtgaacc ttctgtcctg aaccgtgcag gtggtgcgcc
    18961 gacgctggcg gtaggtatcg cgcacgtgtt ccacaaagtg ctgccgatgg ctgacatggg
    19021 cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg cgctggatgc
    19081 gggtacccgt tctggccgct ttatgctgca agacctgctg ggtaacttca tcccgttcct
    19141 gaaaaaaacc gactctctgg ttgctggtat catcggtact gcgggctgtg tgggtctgtg
    19201 gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc
    19261 gctgttcggt atttccaacc agatgctggc agccgtagca ctggtactgg gcaccgttgt
    19321 gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg ctgtatggct
    19381 gcttatctgc accacctggg cgctgggcct gaaactgttc agcaccaacc cgcagatgga
    19441 aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta ctgacctgac
    19501 ggcacagcag attgccaaca tgaaccacat cgttgtgaac aactacacta acgcgggtct
    19561 gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca aaacctggct
    19621 tgcggtgcgt aacagcgaca aacgtactga caaagaaact ccgtacgttc caatcccgga
    19681 aggcggcgtg aagatctctt cgcaccacta accgtgttta gccccgcttc ggcggggctt
    19741 tgttcttatc agagtgaact atgtttggta acttaggaca ggcaaaaaaa tatctcggcc
    19801 aggcggcgaa gatgttgatt ggcattccag actacgacaa ctacgtcgag catatgaaga
    19861 ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa cgccagaacg
    19921 cgcgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga gagcatgaac
    19981 ccgattgcag ttaccctact caccggtttt ttaggcgcgg gcaaaaccac cctgctgcgc
    20041 catattctta atgaacagca cggctacaaa attgccgtga ttgaaaacga attcggcgaa
    20101 gtctccgttg atgatcaact gattggcgac cgcgccacgc agatcaaaac gctgaccaac
    20161 ggctgcatct gctgttcgcg ctccaacgag ctggaggacg cgctactcga cctgctggac
    20221 aatctcgaca agggcaatat tcagttcgac cgtctggtca ttgaatgcac cggcatggcc
    20281 gatcccggcc cgattattca gacctttttc tcccatgaga ttttatgcca gcgttacctg
    20341 ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat gaaccagttc
    20401 accatcgccc agtcccaggt gggctacgct gaccgtattc tgctgactaa aaccgacgtc
    20461 gcaggcgaag tagagaaact gcgtgaacgc ctggcgcgca tcaacgcccg cgcaccggtc
    20521 tacaccgtca cccacggtga catcgacctg ggcctgttgt tcaacaccaa cggttttatg
    20581 ctggaagaaa acgtcgtcag caccaaaccg cgtttccact ttatcgcgga taaacaaaac
    20641 gatatttcgt cgattgtggt ggaactggat tacccggtag atatcagcga agtttcccgc
    20701 gtgatggaaa acctgctgct ggaatcggcg gataaactac tgcgttacaa agggatgctg
    20761 tggattgacg gcgaacctag ccgcctgctg ttccagggcg tccaacgcct ctacagcgcc
    20821 gactgggacc gcccgtgggg cgatgaaaca ccgcatagta caatggtgtt tatcgggatt
    20881 cagctgccgg aggataagat tcgggcggcg tttgcggggt tgaggaagta attttagatg
    20941 atggccgcca tgtggcggcc tgaattattt tttattctga tcgcgcagca tcgcttcacg
    21001 taaaattgcg ttcaggcgag tttgatagcc tttacctggt cgcttcaacc attccatcac
    21061 atcagcatca atacgcaccg aagcctgagt tttcaatggc cgataaaact taccgcgttc
    21121 tgcgttagac cattgctcat cgctggaagg tgggatatca ctgtaatcaa tatcttcgtc
    21181 ggatttattc gccagcgcct tcagttcggc ctcatgctgc acatcgagtg tagaggaggt
    21241 ccctcgttta tgtttaacca tgctcatagc gattccgttc gttgtggatc atcaaacact
    21301 aacaccgcat cttcaaagcg gatgccatgt ttcacccgat tacctttggc cttatttgca
    21361 tcccattcaa actccattgg catagttgcc atccctaatt gtatatacaa aaatgtatgt
    21421 acaattattc tgcaattata gcgttgatgt tggaaatagc atcacgtttc gaataatcaa
    21481 ccatgaaatt gacaatgtgt gagttggact tgattcatcc aatagaattt ctcgcctgac
    21541 atggcacact aaacacctcg ctgtcgttaa gggattaccg tgaaaaacta caaagtcatt
    21601 accccgcttt ttcctaccta tgcgcaagtt aaggctatga tgaaagccgt ctcaggctat
    21661 tccctgaaag cggtgcgcaa tatgattact gccattcacg agcaaaccgg aacgccgcaa
    21721 aaacccgttg actggtcgga acctgacctg tggatcagcg aacgcttaac gggtgaagat
    21781 gccgacatcg cccgtcgtat ttgggatacc gataatcaca tattgaaccc gcgccacagc
    21841 tacggctgct acttattcct caactatccc cagtttgagc tgatggaaag cacgccagac
    21901 gatacctggc agcccacctc gcgcgggcaa aaattcctgc aagatgacga aaaaacgctg
    21961 cgttcactgg acgatcagga aggcattctg caacttcttg aactattagc tggccgcgaa
    22021 atgtcgcgcc gcgccgattt attgccggaa tggcaggcct ttttgcatca gcattcgaaa
    22081 ttcgcgtctg caagttcagt gaaaagtacg ctctactccc gtctctataa ccttatcgac
    22141 cgtgacatgg ttgaccgtga aggcatgagc tatcgcatca ccgataccgg gcgcgcctgg
    22201 cttggaaaag cgttgccggc acgaaaaagc gatccacgta aagagctgct ggaagcggtg
    22261 aaacgctaca acgagcagca aaaagagctg ctgcgcgaac aaatcagcac gatgaacccg
    22321 tacaaatttg aacaactggt tggtcaatta ctggaagcaa tgggctacga gcaggtcgaa
    22381 gtgaccaaag cctctggcga caaaggtgtg gacgtgattg gccaggtgca ggtcggcatc
    22441 accactatta ccgaagtggt gcaggtcaaa agaatgcaga acactatcac tcgcccatat
    22501 atcgaccagt tgcgcggggc gttgccttac cacaaagcca ttcgcggtac actcatcacc
    22561 accgggaaat ttgcggcgaa atgtgcagaa gcggcgctct ttccgggtgc cgcgcccatt
    22621 acgcttatcg acggcgatcg tttactggaa ctgttaatcg aaaataacgt cggcattcgc
    22681 cgcagcaatg cagtggaatt gctggatgtg gatttgcagt tgtttgatga gctggagatg
    22741 gattaaaggg cgaaatccgc cgcctctacc attacaatag cgacccaatt tatctgaaca
    22801 gagttcatca tggccaaagc accaactaaa aaagccaaag caaagaaagg ctttgaagaa
    22861 acattatggg atacagcaaa ccagcttcgc ggtagcgttg agtcctccga atacaagcac
    22921 gtggtgttga gcctcgtgtt cctgaaattc atcagcgata agtttgaagc acgccgcaaa
    22981 aaaatgattg ccgatgggca agcagacttc cttgagatgg aagtgttcta ccagcaggac
    23041 aacattttct acctgccgga agaagcgcgc tggtctttta tcaaacaaaa tgccaaacag
    23101 gacgatattg cggtccgtat tgacaccgcc ctctcgacca tagagaaacg taacccaacg
    23161 ctgaaaggcg cgctgccaga caactacttc agccgtcaga atctggaaac caaaaaactg
    23221 gcgtcactga ttgataccat cgacaacatc gaaacgctgg cacacgaaac tgacgttgaa
    23281 acgttatcga aagaagacct ggtcggacgc gtttacgaat acttcctcgg taagtttgcc
    23341 gcaactgaag gcaaaggcgg tggtgagttc tacacgccca aatgtgtggt tacgctgtta
    23401 actgaaatgc tcgaaccctt ccagggcaaa atttatgacc cgtgctgcgg ctcggcaggg
    23461 atgttcgtgc agtcggtgaa gtttgtcgag agccatcagg gtaaaagtcg tgatatcgcg
    23521 ctgtatggtc aggaactgac agccacgacg tataaactgg caaaaatgaa cctcgccatt
    23581 cgcggtcttt cagctaacct cggcgaacga ccggcagaca ccttctttag cgaccagcac
    23641 ccggacctga aagccgacta cattctggcg aacccgccct tcaacctgaa agactggcgt
    23701 aatgaagccg aattaaccaa agacccacgt tttgctgggt atcgtatgcc gccaaccggt
    23761 aacgccaact acggctggat tttgcatatg ctctccaagc tgtcggctaa cggcacagca
    23821 ggttttgtgc tggcaaacgg ttcgatgagt tctaacacca gcggtgaagg cgagatccgt
    23881 gcacagatga tcgaaaatga tctgattgac tgtatgatcg ccctacctgg acagttgttc
    23941 tacaccacgc agatcccggt gtgtttatgg tttatgacca aatcgaaggc tgccgatccg
    24001 gccaaaggtt atcgtgatcg tcagggcgag acgctgttta ttgatgcgcg taacctcggc
    24061 accatgatta gccgcacaac taaagagtta acggcggaag atattgccac catcgccgat
    24121 acctaccatg catggcgcag tacgccggaa gaactggctg cacggattgc gcgtggtgac
    24181 agcaagctgg aaaaatatga agaccaggct ggcttctgca aagttgcgac cctgcaagac
    24241 attaaagata acgactacgt tctgacaccg ggccgctatg taggtgcagc cgagcaggaa
    24301 gaggacggcg tggcatttga gacgaaaatg cgcgaattgt cgaagacgtt gtttgagcag
    24361 atgaagcagg cggaagaact ggatcgtgcg attcgccaga atctggaggt gctgggttat
    24421 ggggagtaag tggatagaag tatcactggg cgagatttct gaaaaaattg gagatggaat
    24481 ccacggaacg ccaacttata acaattcagg taactattac tttattaatg gaagtaattt
    24541 aattgataat tcaataaaga taactgagac aactaaatgt gttgatcatg atgagtattt
    24601 aaaacaccgt aagaaattga gtaataatac cgttttagtt tcaataaatg gaacc
//
LOCUS       DAZGTJ010000081        24429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000081 DAZGTJ010000000
VERSION     DAZGTJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.081"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..749)
                     /locus_tag="ACHK8E_003770"
     CDS             complement(<1..749)
                     /locus_tag="ACHK8E_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345983.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFITEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNV
                     TGQDLSGFVLTLSRFYFEDLLNINFTDANLG"
     gene            complement(1330..2331)
                     /locus_tag="ACHK8E_003771"
     CDS             complement(1330..2331)
                     /locus_tag="ACHK8E_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0345984.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2438..2734
                     /locus_tag="ACHK8E_003772"
     CDS             2438..2734
                     /locus_tag="ACHK8E_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0345985.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2857..3282)
                     /gene="arsC"
                     /locus_tag="ACHK8E_003773"
     CDS             complement(2857..3282)
                     /gene="arsC"
                     /locus_tag="ACHK8E_003773"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP0345986.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(3295..4584)
                     /gene="arsB"
                     /locus_tag="ACHK8E_003774"
     CDS             complement(3295..4584)
                     /gene="arsB"
                     /locus_tag="ACHK8E_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP0345987.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(4639..4992)
                     /gene="arsR"
                     /locus_tag="ACHK8E_003775"
     CDS             complement(4639..4992)
                     /gene="arsR"
                     /locus_tag="ACHK8E_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP0345988.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(5240..5455)
                     /locus_tag="ACHK8E_003776"
     CDS             complement(5240..5455)
                     /locus_tag="ACHK8E_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0345989.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            5734..5817
                     /gene="dinQ"
                     /locus_tag="ACHK8E_003777"
     CDS             5734..5817
                     /gene="dinQ"
                     /locus_tag="ACHK8E_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP0345990.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5871..7223)
                     /gene="gorA"
                     /locus_tag="ACHK8E_003778"
     CDS             complement(5871..7223)
                     /gene="gorA"
                     /locus_tag="ACHK8E_003778"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP0345991.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7295..8137)
                     /gene="rlmJ"
                     /locus_tag="ACHK8E_003779"
     CDS             complement(7295..8137)
                     /gene="rlmJ"
                     /locus_tag="ACHK8E_003779"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP0345992.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            8340..10382
                     /gene="prlC"
                     /locus_tag="ACHK8E_003780"
     CDS             8340..10382
                     /gene="prlC"
                     /locus_tag="ACHK8E_003780"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP0345993.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            10390..11142
                     /gene="rsmJ"
                     /locus_tag="ACHK8E_003781"
     CDS             10390..11142
                     /gene="rsmJ"
                     /locus_tag="ACHK8E_003781"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP0345994.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11191..12660)
                     /gene="dtpB"
                     /locus_tag="ACHK8E_003782"
     CDS             complement(11191..12660)
                     /gene="dtpB"
                     /locus_tag="ACHK8E_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP0345995.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12975..13409)
                     /gene="uspA"
                     /locus_tag="ACHK8E_003783"
     CDS             complement(12975..13409)
                     /gene="uspA"
                     /locus_tag="ACHK8E_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP0345996.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13800..14135
                     /gene="uspB"
                     /locus_tag="ACHK8E_003784"
     CDS             13800..14135
                     /gene="uspB"
                     /locus_tag="ACHK8E_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP0345997.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14206..15705)
                     /gene="pitA"
                     /locus_tag="ACHK8E_003785"
     CDS             complement(14206..15705)
                     /gene="pitA"
                     /locus_tag="ACHK8E_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP0345998.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15937..17130
                     /gene="yhiN"
                     /locus_tag="ACHK8E_003786"
     CDS             15937..17130
                     /gene="yhiN"
                     /locus_tag="ACHK8E_003786"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0345999.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(17217..18269)
                     /gene="yhiM"
                     /locus_tag="ACHK8E_003787"
     CDS             complement(17217..18269)
                     /gene="yhiM"
                     /locus_tag="ACHK8E_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFP0346000.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            18827..18940
                     /gene="yhiY"
                     /locus_tag="ACHK8E_003788"
     CDS             18827..18940
                     /gene="yhiY"
                     /locus_tag="ACHK8E_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP0346001.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            19003..20070
                     /gene="yhiI"
                     /locus_tag="ACHK8E_003789"
     CDS             19003..20070
                     /gene="yhiI"
                     /locus_tag="ACHK8E_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0346002.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            20067..22802
                     /gene="rbbA"
                     /locus_tag="ACHK8E_003790"
     CDS             20067..22802
                     /gene="rbbA"
                     /locus_tag="ACHK8E_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP0346003.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            22802..23926
                     /gene="yhhJ"
                     /locus_tag="ACHK8E_003791"
     CDS             22802..23926
                     /gene="yhhJ"
                     /locus_tag="ACHK8E_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0346004.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(24125..24429)
                     /locus_tag="ACHK8E_003792"
                     /pseudo
     CDS             complement(24125..24429)
                     /locus_tag="ACHK8E_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         6208 a         6078 c         6335 g         5808 t
ORIGIN      
        1 tcacctaaat ttgcgtcagt gaaatttata ttaagtagat cttcgaaata aaaccttgac
       61 aaggttagaa cgaacccgga aaggtcctgt cctgtcacat tgaaatcaat aaagttggtt
      121 ccagcaaaga ctttcatcct gtttttattt tcactgtgtt caatatataa cttcgtcatt
      181 ttttgataca ggctttgaat atcttttata ttagtcggta agtcagctcg ttgttcagta
      241 ataaaattag tatctgaaaa catggtttgg acactgaatc ttactggtat tccctcttct
      301 acgctaattt tagttatgat tgccttgtcg ggtttaggat tagcgacaac aatgattcga
      361 tattcagcac ttttgttttt ttcgtatcta atgttattaa tctgattaag tattgattgt
      421 gtctgttgcg taagttgtgt cgaacttatc gtttcaatgt tagtaataag acttacaagt
      481 tgagccacaa cttttgagta gtctgcattt gtatatgtag tgttcgtgtg gcacgcatta
      541 atttgttgaa gttggtattg cgctaaatca tattgatcag aggtatatcc ttctccagag
      601 aatgtggaat taatgggttg ttgttcttcc caattatcgc aaggccactt cggtgtgtct
      661 ggtgttttaa cattgtcggt atctgttgtg cggttataaa tatcgccgtg atatgtattt
      721 atcatacatg gggtaaagtc aattgacata acattcctta ttgttcatct atttcgctaa
      781 atttttgtaa atcggagaat tagagcagga atggattttc ttctacgcgg aaataaacac
      841 aatgcgtcat atcttcctga tataattacc tttaacttcc ctttcatttc cacccggtaa
      901 tatttgtgta taaaaaaagg gcagtgatag tatcgtaaag gaaaacgtta acaactatca
      961 aaaagcgctc attttaaaaa gttatgatgt tcaccgatag tgtgtgaagt gatatttttg
     1021 tgatgtttta gatgtgtaat aatgaaccag gttaaagtgt atttaaaaat gtgttgtatt
     1081 ggcgactgaa atctgccctt taaaaggggg gatagcactg aaacaaagag acattgcttc
     1141 tccgataaaa tgtcagagaa gcatgattgc gtggtgattg tcgattgata acttactgac
     1201 ggatccgtca tcattctcaa agcattagtt aagttatttg tgatttttaa ggattatcct
     1261 atctatgatt ttctactcag aaggataagg cactcacaat gctccttata gtttactgct
     1321 ggcatctatt taaaatagca tttgagtcaa atagcccatc aagatagcca tacctaatat
     1381 aaccgtcagg aatgcaacta tcatcggtat tctgaacatt gatttaagta atatcacttc
     1441 cgtcaggctt gcgccggcac tgccgatgat taatgccatt aatgctccca gacccattcc
     1501 ttttgtcatc agaacagatg ccagagggat aaccgcctct gcccggatat atagcggaat
     1561 accgacaacg gcgctcaatg ggatggcgaa gggattatct gcccctgcat gagcggcaat
     1621 ccactctgag ggaataaagc cataaataaa agagcctatt aataccccta acaaaagata
     1681 tggcagtaca tctttaaact gttgcaacgc atctttggtg gctagtttta tgggattaat
     1741 acttaccaca gctttggtac tgcaacagtt tactacaggt ttctcaatgg ctttagccgc
     1801 tggactacag caactcactt ttattggcgt atatgtcgtc cccggcgaag ttttggctgg
     1861 agcacaacaa tttgctgatg agcttttact ggcaatgata tgacgttcaa atcccagaga
     1921 atcgaggata atactggcaa gtacggagac gccggcggcg ataatcgcat acaacaaggt
     1981 aactttccag ccaaaggtca cccacattaa cccgacgata atgggattaa gtaatgggga
     2041 aacaaataaa aaagtgaggg tcggaccaaa cccggctttc gctgataaca atccacgtaa
     2101 catagggatt gtcgagcaac tacagaacgg ggtgacggct cccaacagag cagccaggag
     2161 atagcctttc ccttttcttg cccccatcat ctgctggatt ttatggtctg gcaccttttg
     2221 tcttatcagg ctcacaccgg cactaatcac tataaacagc aaagaaagct cgacggcgag
     2281 aaacacaaac atctctgtgg catcttgcag catcgcaagc caactactca tacataacct
     2341 cattgaataa atctatattt ccagaataat cgaaatgaaa ttcagatcaa gatatttctg
     2401 gtattatcga aatatcaacc agacagaggg ggatcatatg cagttggaag aagtcgctaa
     2461 agcgctgaaa gagctaggtc acccgacccg tttgttcata tttaaacatc ttgtcaaagc
     2521 cggggaacaa gggttgccgg tcggtgagtt gcaaaagcag cttgggatac ccggctctac
     2581 actcagtcac catatttcag cactggtatc ggtagggctg gtcaaacaaa accgtgagag
     2641 tcgaacgctg atgtgtgttt cgcaatacgc aatgctggaa gcgataattg aattcttacg
     2701 cgaggagtgc tgcgtaaata gcaaaattgc ctgatgcgct acgcgctaca gatttcatgc
     2761 catgcacggt tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc
     2821 gcactttacc aactatgggc agtggatatc gccgctttat ttcaagcgtt tacccgcttc
     2881 atcaaccact ttctcgccat cttccttggt gaacgcgcct tgttgcgcat ctggcagaat
     2941 ttccagcacg acttctgaag ggcggcacag gcgagttccc aacggcgtca ccacaatcgg
     3001 gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt
     3061 atcttctgca aggcccagtt cctcatacgg ctcgacgttt ttacgcagca gcgcgcgtac
     3121 ggaaatcccc atatcggcaa tgagtttgac cagttcatca cgcgtcggtg gggtttccag
     3181 ataatagaca atggtcggtt ctgtgccgct gttgcggatc atctccagcg tgttacgcga
     3241 cgtgccgcag gccgggttgt gataaatggt aatgttgctc atatcaatat ctcattacaa
     3301 agtgaaagag agacgtagcg ccagcgcagt cagcgtcaca aacagcacag gcagggtcat
     3361 gataatccct gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac
     3421 gtgcagccag agcagcgtcg ccaggctacc aatcggggta attttcggcc ccaaatcgca
     3481 gccaattacg ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc
     3541 aatggacaac gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc
     3601 ggtgaggaat ccggtgccga gcgtcgcggc ccacaggcca ttatccgcca gcacgttgag
     3661 tacgccagaa agatattccg ttagtccggc attgcgcagg ccataaacca ccagatacat
     3721 gccgagcgag aagatgacaa tctgccaggg cgcaccgcgt agcaccttac tcgtattaat
     3781 cgcatgaccg cgtttagcga cgacaaataa tatcaacgcg cccactgccg caatggcgct
     3841 gaccggaatg ccgagcggtt ccaggacgaa aaatcccacc agcagaaaca gtaaaacaac
     3901 ccagccagtt ttgaaggtgg caggatcttt aatcgcttct gcgggagatt tcagcagcgc
     3961 catatcgtag ttctgcggaa tatctttgcg gaaatagaga tgcaaaatca ccagcgtggc
     4021 aacaattgcg gcgatatcca ccggcaccat caccgatgcg tattcgcgaa agccgaggcc
     4081 aaagaaatct gcggaaacga tattcaccag gttggagaca ataagcggca ggctggcggt
     4141 atcggcaatg aatccggccg ccatcacgaa cgccagcgta gtgcctttac tgaaccctaa
     4201 agccagcaac atggcgatga caatcggtgt caaaataagc gccgcgccat cattggcaaa
     4261 cagagctgca acggcagcac cgagcaggac aatccaggtg aacagcaagc gaccgcgtcc
     4321 attgccccag cgtgagacgt gcagcgccgc ccattcaaaa aagccggact catccagcag
     4381 caggctgatg ataatgacgg caataaacgc cgccgtcgcg ttccagacga tattccacac
     4441 caccggaata tcacccggat ggaccacgcc cgtaactaac gccagtactg cgccgagcgt
     4501 tgcactccag ccgatgccta aacctttcgg ctgccagata accaatacaa tggtcaggac
     4561 aaagatagcg cctgccagta acataatgcc tcccggataa aacacatgcg aaaatacata
     4621 tgtgtttagc taaatttttt aactgcaaat gttcttactg tctgcggaac aattttgtcg
     4681 agccaggttg cggacaatca cctgaacctt ttcctgttca catcgccagg cctcatcaat
     4741 aattttcgcc gcccatgctg gaatatgcgg tgataagcgg taatgaaccc acttaccttg
     4801 cttgcgatcc agcaatagcc cactttcacg cagcaatgac agatggcggg atatcttggg
     4861 ctgcgactga tcgagggcag tgcagagatc gcagacgcat aactctccca gttcgctaag
     4921 cagtaaaacg atgcccagac gggtctcatc agcaagaatt ttgaacagtt gaatgggtaa
     4981 cagaaatgac atattgcgct cctgattgtt gcaggtagtg tctctcttcg aagcggataa
     5041 gtcaaaaaca tatatggttt aacgaatgtg taagtgcaga ggaaggtaat aggtgtgaat
     5101 tttgagttgg gtattcattt gaaaggaggt ctgaatcagg acgtaaaaaa accgactttg
     5161 cgtcggtttt ttactttcca gccctgagtt ggtggctctg gctggagtga gaatgcccac
     5221 atctaacggt tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca
     5281 gctggcggag ggacagtttg ctatacatgt tggtgaaaga ggatttatat actttcatcg
     5341 ggtcgctgcc gtaccaggcc ttgtccgaat ccggatgtaa aaaaccgact ttgcgtcggt
     5401 tttttacttt ccagccctga gttggtggct ctgactggag tgagatagcc atcatctaac
     5461 gcatcaatgc taataccata ctgaaactat tgcaaggaca tgctggtttt ataacccgca
     5521 agtactgtat gattatccag ttagctctga gacattttca ctctggcaat gcgcataaac
     5581 gctttcaaag tcctggtcag aagtacgggt ggtgccgtta actgatgctc tggccggagt
     5641 gagaatgccc acatctaaca atggggcatg cgccgtgaca ggcagtggat gagtaagcgg
     5701 atgcattctc actccatcgc atggagaaaa cgggtgattg ataaagcaat catcgttctt
     5761 ggggcgttaa ttgcgctgct ggaactgatc cgctttctgc ttcagcttct gaactgatag
     5821 cggaaacgta gctaagggct aagagcacac tactcttagc cctttaacat ttaacgcatt
     5881 gtcacgaact cttctgccgc tgtcgggtga atggcaaccg tgttgtcgaa gtcttttttg
     5941 gttgcgccca tcttcagcgc caccgcgaag ccctgcaaca tttcatccat accaaaacca
     6001 atgccgtgaa ttccgacaat cttctcttcc ggtccaacgc ataccagttt catgcggcac
     6061 ggctggcggt gagtggtgac ggcggtatac atcgcggtga aagaggattt atacactttc
     6121 acctgatcgt cgccgtactg ctcgcgcgcc tgcggttccg ttaaaccaac agtcccaatc
     6181 ggcggatggc tgaagaccac ggtcggaatg ttgctgtaat ccagatgctc atccggcttg
     6241 ttattaaaca ggcgttcaga gagacggcga cccgctgcaa ctgccaccgg tgtcagttcc
     6301 actgcacccg tgttatcgcc caccgcgtaa ataccttcaa cgttggtatt ttgatattta
     6361 tcgacgacga tatagccttt ttcgttagtt ttaacgccag cggcttccag gttgatgttg
     6421 tcattggcag gctcgcgacc aatcgcccaa atcaggcaat ccaccgtttc actgcgccca
     6481 tcttccagct ccagcgtcag gctaccatca gcatttttca ctactgcttt cgggatggcg
     6541 ttggtatgca actgcgggcc ttcggcgttc atcacttcga ccagcgtttc agagatcatc
     6601 gggtcgaagc tgcgcagcgg cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg
     6661 ccgttaatca cgcccgccag ttccacggcg atgtaacctg cgccaacaac cgccacgcgc
     6721 tctggcaaag caggaagggc gaagaagcca tcagaatcaa taccgtattc cacgcctgga
     6781 atatccgggt ggctcggacg accgccggtg gcgatcagaa tatgatcggc cgtgatggtt
     6841 tcgccgttta cctccagcgt tttggcatca acgaagcggg caaagccttt gattacatca
     6901 acgttatttt taccgagcac gttttcatag gaagtatgaa tacggtcgat ataggcggta
     6961 cggctggcga tcaacgtttc ccagttgaat ttattgatag tggtatcgaa accataatcc
     7021 ggaccgtaca tgtggatcgc ttcacggatt tgcgccgcgt gccacatcac ttttttcggc
     7081 acgcagccga cgttcacgca ggtgccgccc agttctttgg cttcaatcag cgcgcatttc
     7141 tgaccgtaca tagccgcgcg gttgatggag gcgataccgc cgctgccgcc gccgatggcg
     7201 atgtaatcat agtgtttagt catgacatat tgtccttatc gttgattacc gcgattgtag
     7261 cgcgagacgt aataggtgcc agcgatggct gcaattactc cggcacgatc cagcttgcgg
     7321 tggcgtgccc agtgccagct ggaaccagtt tactgtgcag ccacggcagc acgttattca
     7381 tctgctgttc cagcttccac ggcggattaa tcacaatcat gccggaagcg gtcatgccac
     7441 ggcgatcgct gtctggcagc accgccagtt caatttgcag aattttgcga atgccggtcg
     7501 cttccaggtc gtggatcatg cgcttaattt gctggcgcag caccactgga taccacaatg
     7561 cgtaagtgcc agtggcgaaa cgtttgtaac cttctgctat cccgctgacc accgcctgat
     7621 agtcggtttt catttcatac ggcgggtcga taaggattaa accacggcgg gaaaccggcg
     7681 gcagcttggc cttaagctgc tggaaaccgt cggctttttc gacgcgcgca cggctgtctt
     7741 tctgaaattc agaacgcagc aacgggtaat cgctcgggtg cagctcggtc agttgcaggc
     7801 tgtcctgttc acgcagtagc tggcgagcaa tcaacggcga accggggtaa taacgcaact
     7861 gcccgctacg gttgaagtgt tttaccacat tgatgtacgc ctccagctcc gcaggcaaat
     7921 cgtcctgctg ccagatacgg gcgatacctt cgagatattc gccggtacgc tcggcatgtt
     7981 cgctgcttaa ctgataacgc ccggcccctg cgtgggtgtc gagatagaga aacggtttat
     8041 ctttctcttt cagcgactcg atgatcaggc tctgaacggt atgtttaagg acgtcggcgt
     8101 ggttgccagc gtgaaagctg tggcgataac tgagcatggg taaaggtgtt ccggtaagta
     8161 aaaatcggcc cgtattcggg gcgcacaaaa gcctatcagg acagtatacc gaaaagaggc
     8221 cgccgccgcg aaagcgtaac gtttctcatt gaaattcact acacttaacc ccatgctaca
     8281 cacattatgt aaagcgcctg ttgagcgctt ccttaacctc tttaaccagg actgcgctaa
     8341 tgacgaatcc gttactgact ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac
     8401 atgtcgttcc agccgtgact aaggcgctga atgactgccg cgaaaacgtg gagcgcgtag
     8461 tagcgcaagg ggcaccgtac acctgggaaa atctctgcca gccgttggca gaagtagacg
     8521 atgtgctggg gcgtatcttc tccccggtca gccacctgaa ctcggtgaaa aatagcccgg
     8581 aactgcgtga agcgtacgaa caaaccctgc cgctgctgtc agaatacagc acctgggtag
     8641 ggcaacatga agggctgtat aaagcgtatc gcgacctgcg cgatggcgat cattacgcca
     8701 cgctgaacac ggcgcagaaa aaagcggttg ataacgcact gcgcgacttc gaactctctg
     8761 gcatcggtct gccaaaagag aaacaacaac gctacggtga gattgccacc cgtctttccg
     8821 agctgggcaa ccagtacagc aacaacgtcc tcgatgcgac aatgggctgg accaaactcg
     8881 ttaccgacga agcggaactg gcaggaatgc cagaaagcgc actggctgcg gcaaaagccc
     8941 aggctgaagc gaaagagctg gaaggttatt tgctgacgct ggatatccca agctacttgc
     9001 cggtaatgac ttactgcgac aaccaggcct tgcgtgaaga gatgtatcgt gcttacagca
     9061 cccgcgcttc cgatcaaggc ccgaacgccg gtaagtggga caacagcaag gtgatggaag
     9121 agatcctcgc gctgcgtcac gaactggcgc aactgctggg ttttgaaaac tacgccttta
     9181 aatcccttgc cactaaaatg gcagaaaatc cgcagcaggt gcttgatttc ttaaccgatc
     9241 tggcaaaacg cgcgcgtccg caaggtgaaa aagagctggc gcaactgcgt gccttcgcta
     9301 aagccgaatt tggcgtcgat gagttgcagc cgtgggatat cgcttactac agcgaaaaac
     9361 aaaaacagca tctctacagc atcagtgacg aacagctgcg tccgtacttc ccggaaaaca
     9421 aagcggttaa cggcttgttt gaagtggtga aacgtattta cggcatcacc gctaaagagc
     9481 gtaaagatgt tgatgtctgg catccggatg tacgtttctt cgaactgtat gacgagaaca
     9541 acgaactgcg cggcagcttc tacctcgacc tgtatgcccg tgaaaacaag cgcggcgggg
     9601 cgtggatgga tgactgcgta ggccagatgc gtaaagccga cggttcgctg caaaaaccgg
     9661 tcgcgtatct gacctgtaac ttcaaccgcc cggtaaatgg taaaccggca ctgtttaccc
     9721 atgacgaagt gatcaccctg ttccatgaat tcggtcacgg cctgcaccat atgctgaccc
     9781 gcattgaaac tgctggtgta tctggtatca gcggggtgcc gtgggatgcg gtcgaactgc
     9841 cgagtcagtt tatggaaaac tggtgctggg agccggaggc gctggcgttt atctccggtc
     9901 actatgaaac cggcgaaccg ctgccgaagg agctgctgga taaaatgctg gcggctaaga
     9961 actaccaggc ggcgctgttt attctgcgcc aactggagtt cggcctgttt gatttccgcc
    10021 tccatgccga gttccgcccg gatcagggag cgaaaatcct cgaaactctg gcagaaatca
    10081 agaaactggt tgccgtagta ccgtctccat cctggggccg tttcccgcac gctttcagcc
    10141 atattttcgc cggtggttat gccgcaggtt actacagcta cctgtgggcc gacgtgctgg
    10201 cggcagatgc cttctcgcgt tttgaggaag agggcatttt caaccgtgaa actgggcagt
    10261 cgttcctcga caacattctg atccgtggcg gttcagaaga gccgatggag ctgttcaaac
    10321 gcttccgtgg tcgtgaacca cagctggatg cgatgctgga gcattacggc attaagggct
    10381 gaacattcag tgaaaatctg cttaattgat gaaacaggcg ccggagacgg cgccttatct
    10441 gttctggcgg cccgctgggg gctggagcac gatgaagaca acctgatggc gctggtgtta
    10501 acaccggagc acctggaact gcgcaagcgt gatgagccga agctgggcgg catctttgtt
    10561 gattttgttg gcggggcgat ggcgcaccga cgcaaattcg gcggtggtcg cggagaggcg
    10621 gtggcgaaag cggtgggcat taaaggcgat tatttgccgg atgtggtgga cgccaccgca
    10681 gggctggggc gcgatgcctt tgtgctggct tcggtcggct gccgcgtgcg gatgctggag
    10741 cgtaatccgg tggttgctgc attgctcgac gacggcctgg cgcgtggtta tgcggatgcg
    10801 gaaatcggcg gctggttgca ggagcggttg cagttaattc acgcttccag cctgacggcg
    10861 ctgaccgata ttactccgcg cccgcaggtg gtctatctcg atccgatgtt cccgcataag
    10921 cagaaaagcg cgttggtgaa aaaagagatg cgtgtctttc agtcgctggt ggggccggat
    10981 cttgatgccg atggattgct ggagcctgca cgcttgctgg cgaccaaacg cgtggtggta
    11041 aagcgcccgg actacgcgcc gccgctggcg aatgtcgcca cgccaaacgc ggtagtgacc
    11101 aaagggcatc gctttgatat ttatgccgga acgccggtgt aaaagtaaag cagggtagcg
    11161 ttatcgctac cctgctttct gcaagaataa ttaatggctt tcgggcgtcg caatcatgcg
    11221 tttcagccac ggcaccatca gcagcatcac caccgcaacg cccagcgtaa ccaggccaat
    11281 cttaccaaac acgttggtat agacgggcaa cgtctcaagc ggatcggtaa tgttgtctgg
    11341 tactgcggta aatgttgcca catagccgcc cagcaagaac gcggcagcct gcgtcaggaa
    11401 ccacatcccg agaataaagc ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag
    11461 acccagggcg ctaataaaca attcacccag gctctggaac aagtaaacca gcacgataaa
    11521 ccacggcgat gtcagtcctt gtgcatcagc aaaccacatt cccgcagcgg cggctgtaag
    11581 aaagcccagc gagcacataa acatgccgag agtaaatttc atcggcatcg agaggtcttt
    11641 gcctttgtta cccagatgcg tgtaaatgcc tgccagtatt gggctggcga gtaccaccca
    11701 gaacgggtta agcgcctgga agctgactgg gttgatggaa aagccgagaa tttcatgatg
    11761 cacgttgttg atagcaaaga agttcagcga tgttggcatc tgggcgtaga gaatgtaaaa
    11821 caccaccgct tcgagcatca ggacaaaggc gacaaacatt ttattgcgcc cggttttatc
    11881 cagcttgaat gcctgacgaa agaaaatgat ggtgacgacg atggagagaa caatcagcac
    11941 cagattggcg acttctacgt tgtgcatcag ccatgcacag acgaagatca tcaccacgct
    12001 gccaagtaac acgtacaaca gtttgctgaa gctcatcggc cggaagtcgg gttcagaacc
    12061 aatgtctttc accattccgc gacaggcgat gtaaaccaat agcgcgataa ttaatcccgc
    12121 accgcacagg ttgtaggtga ctgaatagcc gaatctatca gcgatcacag gggccagcga
    12181 taacgctatc aacgagccga tgttgatcga catatagaac agggtaaatg cgccatcaag
    12241 ccgcggatct ttcggcggat agcacttcga aagcaagctg gctgggttag ctttaaacag
    12301 gccgttaccg acagcgatag tccccagggc gatgaaaatc aggtcaggct taagtagcga
    12361 caggccggtc atgaagtagc caatcgccag cacaagtgct ccgaggacaa tggtgcgttt
    12421 ggtccccagc aggtggtcgc cgacatagcc gccaatggaa atgaggccat agaccagcgc
    12481 agcaaaagca ccaaaagtga caaaagcctg ttcttgcgag aatccaagct gcttaacgaa
    12541 gaaaaccgcc agtacgccct gcacgccgta gtagccgaat cgctcccata actcgacaaa
    12601 aaagatcatg aaaaatgggc gaggttgctg cagcatcccc atgggtgttg ttgtattcat
    12661 atttattaac cttccaatac catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa
    12721 aaagtttaca tctgtcagag attcttaata ttgatgcggt gaatagatta atacactatt
    12781 acaacagaaa ataaccagct gatcacacta atgatggaaa atatgatggt gttaaatgtt
    12841 gtattggcag gggattttga ttgcgtacgc agaccgtggc cagataaggt gtttacgctg
    12901 caaggtaaca aggcgcgcca aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg
    12961 tcgtagaggg aagattattc ttcttcgtcg cgcagcggaa caatcagcat atcaacgtga
    13021 acggtgttga tcagctgacg tgcggaagac atcagtttgc tccagaagtc ctggtggtga
    13081 ccacaaacca ccaaatccat atcgtatttc ttgattgcat cgaccagaac ctggcccagg
    13141 tcgccgctgc cgctcagggt ttcagtgatt gggtagcctg cattagtgga aagctcggtc
    13201 agcgcatgat gtgtctcttc agagatgcgt ttctgcatat cacccagatt cacatcaata
    13261 agcccggtgt ataggtcaga gtagtttaca tctacgtgga tcagagaaac tttcgcattg
    13321 taggggcgag ccatagagac tgctttctct accagaactt tgctttccgg ggagagatcg
    13381 accgcgatga gaatgtgttt ataagccata gtgttactcc ttccataaag ttgtcgatga
    13441 ctggccagct agcgtttctt gtgccgttac gtcacccgcg tcctgcgaac tacgtgcgcg
    13501 gggctaaccc agccgtaaag attcagtgtt aagaccatcc ttaccttata gcgacccgga
    13561 tgatccgtca atccgccttg cttacccgtt agataaacaa attttcgaca tgaatatatt
    13621 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
    13681 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
    13741 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
    13801 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
    13861 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
    13921 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
    13981 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
    14041 gccgctgtga gcgggtgcgt cggcagttta ttctgaccag cgcattgtgt ggtctggtgg
    14101 tggtcagtct gattgcattg atgatttggc attaagcact tcagcaaaaa taacgcataa
    14161 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tgcgattaca ggaactgcaa
    14221 ggagagccag tacagcccgc cggaaagcag taccgcagcc ggaagggtaa acacccaggc
    14281 catcagaata ctggtcacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
    14341 cgcgacagaa gaggagagta cgtgggtagt ggaaaccggc atcccggtat aactcgccag
    14401 gccgatagac actgccgcag tcatctgggc agacattccc tgagcgtagg tcatgccttt
    14461 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
    14521 acctaacgcc agcgcgaccg ccatgatgat ccacaccggt gcatactcga tggtgctaag
    14581 catgtcagac ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc caggcatctt
    14641 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
    14701 atcaagcgac agcttatcgt agctttccac atcggtagtc aacatgcctt tcaggcggtt
    14761 gagcgcgtta atggtattcg acggatggca atggaactcc gcaggttgcg ttgcgccagc
    14821 ttccggagcc ggtactaact gatcagcacc ggtcgcttgt ttcagcagcg caggatgctg
    14881 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
    14941 ggcattcatg ttcaccacga agcctgctgg tgcgacacca atcaatacca acataaccag
    15001 accaatgcct ttctgaccat cgttcgcgcc atgcgaaaac gccacgccga tagcggaaag
    15061 gatcagcgca atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
    15121 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
    15181 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    15241 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    15301 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    15361 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    15421 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    15481 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    15541 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    15601 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    15661 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    15721 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    15781 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
    15841 attcaggtaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    15901 acaattcacg cccgtttttt cttagaggag cgcaaagtgg aaaggtttga tgccattatt
    15961 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggc aggacgccgg
    16021 gttctgctga tcgataatgg taaaaaacca gggcgcaaaa tcctcatgtc tggcggtggg
    16081 cgctgcaact ttaccaacct ttatgtcgaa ccgggcgctt atctgagcca gaatccgcat
    16141 ttttgtaagt ctgcactcgc gcgttttacc cagtgggatt tcattgatct ggtcaataaa
    16201 cacggcatca cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
    16261 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtgacctt cagattgcgt
    16321 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgaact gaacggcatg
    16381 actgtcggtt gcgaaaagct ggtcatcgcg accggtggac tgtcaatgcc agggctgggc
    16441 gcgtcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
    16501 gcgggcctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
    16561 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca tcgttttccg tgagaactta
    16621 ctcttcaccc accgcggctt atctggaccg gcggtgttgc aaatttccag ctactggcaa
    16681 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    16741 gagcagcgta atgcacatcc gaatcagagc ctgaaaaaca cactggcggt tcatctaccg
    16801 aagcggttgg ttgaacgttt acaacaactc gggcaaatcc cggatgtttc gctaaaacag
    16861 ctcaacgtgc gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
    16921 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
    16981 ctctcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg
    17041 atggacgtca ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    17101 gcttgtgcgc aggatttggt ggcagaataa gaagcaaaat gtgctctcga taccagaggg
    17161 attaacctga ataaaaccag ataaggcgtc caacgcctta tctgaagcct tttttattat
    17221 tttttcgctg aacccgcttc taatatagaa acgatggaaa acagtgtaaa acaaactgca
    17281 cctaagccaa ccagaactcg cgaaggaata aagtaagcca tatcggtttg cgcaatttca
    17341 gccaggaacg cagcaaggaa cagacaaaac agacaggtaa ataccggaat catgggtatt
    17401 ctgttggcta acgaacaggt gcgtcgccat accagcgcta acaaccacac tttggagaaa
    17461 atactgtagc agatcatccc caggcaaatg agaataatgc cgggggccag gcgggcgctt
    17521 gcatctgaac tgattaatac gtagatacca aaaataatgg taagtgagcc aagcaaaata
    17581 acccaatagc accacagcca atgttctttc actgagaacg tattccgcgt ttgatggaca
    17641 atagtggcga caaggccaat aagacaagcg caaattgccg ttaaccccat tagtacgtga
    17701 cctgcaacat aatgtggtgt gatatctgca ctacgtaaca gtatgactga ccaaatgaaa
    17761 ccaaagagag taagcagaac gggaacggca attaagcaat taccgattgt tgaagaatag
    17821 gcctgcaatg gcgtaccgtc actcttactg cccgaggcgt ttttggggat caacagaaaa
    17881 tgtccggaag atgccgctac ggttgataca caagcggcaa tcatgccaac gccgaaaata
    17941 acatgaccgg cgacaaattc atctgccatc acattattgc ttgccaacag tgaccatccc
    18001 caaatcatag ttgtagcaga accggcataa ccaataacgg gaaataacct gttgtagaat
    18061 ttattcatgc cgtgagttaa ctgagatata ataataaatg cggtagtgaa caatgccaga
    18121 catatcgcag aaagagaaat cagaacatgc cctgcaacga aatattcact ttgcccagaa
    18181 ccggttaata caaactcacc taatgcgatg catattatac ccatcagtaa aggaattaat
    18241 ttaaacaacc atccgatata tatattcatt ttaaacccca tatatatttt ctgctacact
    18301 tcgaaaaaca tatgattcgt cttttcagtt agttaaatat gtctataaga tgccatcaat
    18361 taaaaaaagt aacttcacta taatcggatt tagcgttatg gggatcaact tttagcggtg
    18421 agttaatgct attaaataaa tatttatcct aagataaact ctgaattaat atctttatct
    18481 tattttgttg ttttaaatga gtcttttagg gaatatttta ttaaacccac gagcctttat
    18541 catctatcat cttttcagaa taaattattt tatattcggt atttattcgc acatcaggcg
    18601 ataataacgc tatgtgatag tcatcaggtt tccctgttac ggaaacctgt gcaacagaat
    18661 ctgtgaacct gtacagaata tttttggaaa aaacaccagc gaacggaaga acatcgcgat
    18721 gtatcacaag ttgatggcaa tcaaatagac aacatgaata taactgttag tatttaattg
    18781 atttcactta tgagtatgcg tgtttcattt acggccagga atgctgatga tgacaacatt
    18841 actacccgtt ttcactaagc cttccccatt ggcgctcaat gctctgtgcg ctggtcgtat
    18901 ttgccatttc cttcttatcc cggatggaag aatccgctga acatcgttcg gtggcagttg
    18961 taacacgcgt cgattgctta cagggattat ggcggatcga ttatggataa gagtaagcgc
    19021 catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc gtggtggctg
    19081 ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat tgaagcgacg
    19141 gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt gaaagaaggc
    19201 cagtttgttc gagaaggtga agtgctggcg aagatggata ctcgcgtgtt gcaggaacag
    19261 cgactggaag ccatcgcgca aatcaaagaa gcacaaagtg ccgttgccgc cgcgcaggct
    19321 ttgctggagc aacgccaaag cgaaactcgt gccgcacagt cgctggttaa tcaacgccag
    19381 gcagaactgg actccgtagc aaaacgtcat acgcgttccc gctcactggc acaacggggg
    19441 gcaatttctg cgcaacagtt ggatgacgac cgcgccgccg ctgagagcgc ccgagctgcg
    19501 ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc ggcacgcacc
    19561 aatatcattc aggcgcaaac tcgcgtcgaa gcggcacaag ccactgaacg gcgcattgcc
    19621 gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt
    19681 gccgagccag gcgaagtgct ggcggcaggc ggtcgggtgc tgaatatggt cgatctcagc
    19741 gacgtctata tgaccttctt tctgccaacc gaacaggcgg gcacgctgaa actgggcggt
    19801 gaagcacgtt tgattcttga tgctgcacca gatctgcgta ttcccgcgac catcagtttt
    19861 gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga gcggctgaaa
    19921 ttgatgttcc gtgtcaaagc gcgtatccca ccggaattac tccagcagca tctggaatat
    19981 gtcaaaaccg gattgccggg cgtagcgtgg gtgcgggtga atgaagaact tccgtggcct
    20041 gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc gtcccgcctg
    20101 tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg aacaatatca
    20161 cactcgatat tccggcccgt tgcatggtcg ggctgattgg accggatggt gtcggtaaat
    20221 cgagcttgtt gtcgttgatt tccggtgccc gcgtcattga gcagggcagc gtgatggtgc
    20281 tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ttgcccgcgc atcgcctgga
    20341 tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa aacgtcgatt
    20401 ttttcgctcg cctgttcggt cacgacaaag cggagcggga agtgcgaatc aatgagctgc
    20461 tgaccagcac cgggttagca ccgtttcgtg atcgtccggc agggaaactc tccggcggga
    20521 tgaagcaaaa acttgggctg tgctgtgcgt taatccacga cccggaactg ttgattcttg
    20581 atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat ttgatcgaca
    20641 gcattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctac atggaagagg
    20701 ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca accggcagcg
    20761 ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt ataaatctgt
    20821 taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat caccctgaaa
    20881 acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc ttcgttgccg
    20941 ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctc ggttcgaacg
    21001 gctgcggtaa atccaccacc atgaaaatgc tcaccggact gctgcccgcc agcgaaggtg
    21061 aggcgtggct gtttgggcaa ccggttgatc caaaagatat cgatacccgc cgtcgggtag
    21121 gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa aaccttgagt
    21181 tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg gctgaaatga
    21241 gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg ccgctcggca
    21301 ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg ttaatcctcg
    21361 atgagcctac ttctggcgtc gatccggtgg cgagggatat gttctggcag ttgatggtcg
    21421 atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg aacgaagcgg
    21481 aacgttgcga ccgtatctca ctgatgcacg ccggaaaagt gctcgccagc gggacaccgc
    21541 aggaactggt tgagaaacgc ggagccgcaa gtctggaaga ggcatttatc gcctgtttgc
    21601 aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtgatacac gacaccaccc
    21661 acgcggcgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc cgcgaagcgc
    21721 tggaactgcg acgcgatccg gtacgttcga cgctggcgct gatgggaacg gtgatcctga
    21781 tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt gcggtgcttg
    21841 accgcgatca aaccgtcagt agccaggcgt ggacgctcaa tctctccggt tcccgttact
    21901 ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg cgtgcgggcg
    21961 atatcacggt ggcaattgag atccctccta atttcgggcg cgatatcgcg cgtggtacgc
    22021 ccgtggaact cggtgtctgg atcgacggtg cgatgccgag ccgcgccgaa acggtaaaag
    22081 gctacgtgca ggccatgcac cagagctggc tacaggatgt ggcgagccga cagtccacac
    22141 ccgccagcca aagcgggctg atgaatattg agacgcgcta tcgctataac ccggacgtaa
    22201 aaagcctgcc ggcgattgtt ccggcggtga tcccgcttct gctgatgatg gtcccgtcaa
    22261 tgctaagcgc ccttagcgtg gtgcgggaaa aagagctagg gtcgattatc aacctttacg
    22321 tgacccccac cacccgtagc gaatttttgc ttggcaaaca gctgccatac atcgcgctgg
    22381 ggatgctgaa ctttttcctg ctctgcgccc tgtcggtatt tgtatttggc gtaccgcata
    22441 aaggcagttt cctgactctc accctggcgg cgctgctgta tatcatcatc gccaccggaa
    22501 tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttt ggtacggcaa
    22561 ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta gcttcgctgg
    22621 aaggtccagg acgttggatc ggcgaggttt acccgaccag tcattttctg actatcgccc
    22681 gcggaacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt atcccgttgc
    22741 tgatagccat accgctggtg atgggcttaa gcatcctgct gctgaaaaaa caggagggat
    22801 gatgcgccat ttacgtaata tttttaatct gggtatcaaa gagttgcgca gtctgctcgg
    22861 tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt attcgtcagc
    22921 gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata tggatcaatc
    22981 gcagttatcg aaccggatcg ttaacagctt ctatcgcccg tggtttttgc caccggagat
    23041 gatcaccgcc gatgagatgg atgccgggct ggacgccgga cgttatacct tcgcgataaa
    23101 tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata ttcaggtgaa
    23161 cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc agaatattat
    23221 caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac cgttggtatc
    23281 gctggaaaca cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg gcggggtgat
    23341 ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg cgctgatccg
    23401 cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc cgtttgagat
    23461 catgatggca aaggtctggt cgatggggct ggtggtgctg gtggtgtcgg gattatcgct
    23521 ggtgctgatg gtaaaaggcg tgctgggcgt accgatagaa ggctcgatcc cgctgtttat
    23581 gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggtattttta tggggacgat
    23641 agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc cgctgcaaat
    23701 gctttccggt ggttccacgc cgcgcgaaag tatgccgcag atggtgcagg acattatgct
    23761 gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc gtggtgccgg
    23821 attcgaaatc gtctggccgc agttcctgac actgatggca attggcggcg cgtttttcac
    23881 catcgcactg ctgagattca ggaagacaat tgggacaatg gcgtaattac agttgcccga
    23941 tgcgtaagct tatagggtct acgcattggt gcaatttatt gaattttagt tttttttagg
    24001 ccggataagg cgtttatgaa ctgaccacca aaacctggac agtttcagtt aaccggcttt
    24061 tttatactca gtccggtatt cagggcaaga ttacgaaagc ccgctccccg caaggactga
    24121 cgcgagaaat gacatgaatc gctcccctgc ggcgactctt gtcataagag tgccggagcg
    24181 tttttccatc aattacaatg acgtctttat catctgaaga atggcagtca cgcatccagt
    24241 taataaagca ctcgtgaaat ttttcaggac tgatacagga tacaactctg gcaatggtat
    24301 cgtgaacagg aataccattt tcaaaatcac catattgctt caaaaaatcg agatgagttt
    24361 ccccaaaatc ctctatatct tcccaacctt ctgcaccaga aataacggca caaatagtca
    24421 acagtagaa
//
LOCUS       DAZGTJ010000082        24276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000082 DAZGTJ010000000
VERSION     DAZGTJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.082"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..250)
                     /locus_tag="ACHK8E_003793"
     CDS             complement(<1..250)
                     /locus_tag="ACHK8E_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP0346005.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLV"
     gene            complement(247..1917)
                     /gene="menD"
                     /locus_tag="ACHK8E_003794"
     CDS             complement(247..1917)
                     /gene="menD"
                     /locus_tag="ACHK8E_003794"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP0346006.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2006..3301)
                     /gene="menF"
                     /locus_tag="ACHK8E_003795"
     CDS             complement(2006..3301)
                     /gene="menF"
                     /locus_tag="ACHK8E_003795"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP0346007.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3380..3685)
                     /gene="elaB"
                     /locus_tag="ACHK8E_003796"
     CDS             complement(3380..3685)
                     /gene="elaB"
                     /locus_tag="ACHK8E_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP0346008.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3740..4201)
                     /gene="elaA"
                     /locus_tag="ACHK8E_003797"
     CDS             complement(3740..4201)
                     /gene="elaA"
                     /locus_tag="ACHK8E_003797"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0346009.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4266..5183
                     /gene="rbn"
                     /locus_tag="ACHK8E_003798"
     CDS             4266..5183
                     /gene="rbn"
                     /locus_tag="ACHK8E_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP0346010.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5371..5484
                     /locus_tag="ACHK8E_003799"
                     /pseudo
     CDS             5371..5484
                     /locus_tag="ACHK8E_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            5539..6510
                     /gene="yfbL"
                     /locus_tag="ACHK8E_003800"
     CDS             5539..6510
                     /gene="yfbL"
                     /locus_tag="ACHK8E_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFP0346011.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            6613..7116
                     /gene="yfbM"
                     /locus_tag="ACHK8E_003801"
     CDS             6613..7116
                     /gene="yfbM"
                     /locus_tag="ACHK8E_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP0346012.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(7183..8640)
                     /gene="nuoN"
                     /locus_tag="ACHK8E_003802"
     CDS             complement(7183..8640)
                     /gene="nuoN"
                     /locus_tag="ACHK8E_003802"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP0346013.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(8647..10176)
                     /gene="nuoM"
                     /locus_tag="ACHK8E_003803"
     CDS             complement(8647..10176)
                     /gene="nuoM"
                     /locus_tag="ACHK8E_003803"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP0346014.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(10340..12181)
                     /gene="nuoL"
                     /locus_tag="ACHK8E_003804"
     CDS             complement(10340..12181)
                     /gene="nuoL"
                     /locus_tag="ACHK8E_003804"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP0346015.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(12178..12480)
                     /gene="nuoK"
                     /locus_tag="ACHK8E_003805"
     CDS             complement(12178..12480)
                     /gene="nuoK"
                     /locus_tag="ACHK8E_003805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP0346016.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(12477..13031)
                     /gene="nuoJ"
                     /locus_tag="ACHK8E_003806"
     CDS             complement(12477..13031)
                     /gene="nuoJ"
                     /locus_tag="ACHK8E_003806"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP0346017.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(13043..13585)
                     /gene="nuoI"
                     /locus_tag="ACHK8E_003807"
     CDS             complement(13043..13585)
                     /gene="nuoI"
                     /locus_tag="ACHK8E_003807"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP0346018.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(13600..14577)
                     /gene="nuoH"
                     /locus_tag="ACHK8E_003808"
     CDS             complement(13600..14577)
                     /gene="nuoH"
                     /locus_tag="ACHK8E_003808"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP0346019.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(14574..17300)
                     /gene="nuoG"
                     /locus_tag="ACHK8E_003809"
     CDS             complement(14574..17300)
                     /gene="nuoG"
                     /locus_tag="ACHK8E_003809"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP0346020.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(17353..18690)
                     /gene="nuoF"
                     /locus_tag="ACHK8E_003810"
     CDS             complement(17353..18690)
                     /gene="nuoF"
                     /locus_tag="ACHK8E_003810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP0346021.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(18687..19187)
                     /gene="nuoE"
                     /locus_tag="ACHK8E_003811"
     CDS             complement(18687..19187)
                     /gene="nuoE"
                     /locus_tag="ACHK8E_003811"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP0346022.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(19190..20992)
                     /gene="nuoC"
                     /locus_tag="ACHK8E_003812"
     CDS             complement(19190..20992)
                     /gene="nuoC"
                     /locus_tag="ACHK8E_003812"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP0346023.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(21086..21748)
                     /gene="nuoB"
                     /locus_tag="ACHK8E_003813"
     CDS             complement(21086..21748)
                     /gene="nuoB"
                     /locus_tag="ACHK8E_003813"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP0346024.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(21764..22207)
                     /gene="nuoA"
                     /locus_tag="ACHK8E_003814"
     CDS             complement(21764..22207)
                     /gene="nuoA"
                     /locus_tag="ACHK8E_003814"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP0346025.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(22837..23775)
                     /gene="lrhA"
                     /locus_tag="ACHK8E_003815"
     CDS             complement(22837..23775)
                     /gene="lrhA"
                     /locus_tag="ACHK8E_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP0346026.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         6004 a         6696 c         6197 g         5379 t
ORIGIN      
        1 ccaccagcca gaagttaagg atgttgtaac taactaaggt tttacgcagt aagtcggtga
       61 catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc gtgacctggg agatcaacat
      121 acaaccgtga gtagtcggca aacgcctcgc ccacttcttg ccattcatgg caatcgccgg
      181 aaaaaccgtg caaaaacacc agccagggta aacctggttt accgtgtttt gcctgcgcgt
      241 gcaggatcat aaatggctta cctgcgccag cagttgctgg agggtttgcg caccatcggt
      301 gtcgttaacc accatttcaa ttaccgtggt ggtcggcgtg cgccaggcgt cggcaagtgc
      361 cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcgaaca tcgcggcggc
      421 gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcacgctcgt ttttcggcgt
      481 tggcaacagc gagaaaattt gcccgccgtt gttgttcacc acaattaata ccagcggcgc
      541 ggaaacctga cgtaataacg ccagcgcgtt gagatcgtaa agtgctgaga gatcgcccac
      601 aatcgccagc gtcggtttgc cactggcccg ctgaacgcca gcggcggtcg agagcagccc
      661 gtcgataccg ctggcaccac ggttgctgta caccgggtaa cctgccggaa gttgcgaaag
      721 cgcatcaatc agacgtacca ccaggctgtt accaacaaac aattgaccct gttcaggcag
      781 atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac gggcaataac
      841 cgcctgcatt gcctgttccg ccaggcgcgg aatttcaacg caccagggct ggcgtttttc
      901 tgccggatgc tgctccagcc agtcggcaat attggcaatt aagcgacgtc cacgatggtg
      961 tgccggatca agtcgccctt cgatgtcatc aacaatccag tactcttccg gttcacagct
     1021 tgcctgccat tgcaggagcc gtttgcccgt caggctgctt cccagttgca ccacaatttg
     1081 tgcctgttgc agttcgctgg tcgctttggc attgcctaac cagagatcgg cacacggcag
     1141 cggctgtccg gtttgcgaca gcacgtcgcc aatcagcggc cagccaagag tttgcgccca
     1201 cagtgcaact tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgctt
     1261 ttgccgccag aagaaccagt cgcgctgttt ttcactttcc agacgaggcg cttcacgcag
     1321 ccacggtttg tcgtcctgcc accagtcgcc cagccgctgt tgccagctaa gcccggtgtc
     1381 gtccatttcg ccatacagcg gttcagcaaa cgggcagttg atatggactc cgcccgcatg
     1441 aagcgtaccg agagcatggt cgatggtaga aaccagccaa cgtgcaggaa tatcctgagt
     1501 cgggcgcggc agtgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctgacgaat
     1561 cgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta acaggatcag
     1621 tttttctccg gttaacccag cttcaatcag tgccggatag agatttgcta ccgccgtgcc
     1681 ggaggtcaca atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccaaatg
     1741 ccccagtcca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc
     1801 taacgttaac ggtgtagaac gcgagccagg ggcgatacag atgtgtctga cgccgtgacg
     1861 cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag
     1921 tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat
     1981 gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc agccctgccg
     2041 ctttgttatc gatttcctgc cactcttgct cggggtcaga accacggaca atgcccgcgc
     2101 cagcatatag ccgcacgaca ttgccactga tttttgctga gcgcagggaa acgcagaatt
     2161 cgctttgttg taaagagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt
     2221 cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg
     2281 gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgcagtc cagatacagc
     2341 ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccaacgtct
     2401 gggtatcggc ctgcaatcgt tggcagatat cctccaccac caacatgttc tcgcgctggt
     2461 ttttatcatc cgccatcagc cactcaccta actgctgcgc ctgcttatca tcaggatgat
     2521 ttgctaccgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc
     2581 gttccggtga agagccaaga aaagcaattt cgccatcaaa ggccatgtaa aaatggtagc
     2641 aattcagatt cagtcgacga ctggcagcca tcatcgccgc cgcattgacc ggacttgcga
     2701 aatgcaggtc agttgcccga gcaagcacca ctttgtcgag ctcaccttcg gcgatggttt
     2761 tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcggg
     2821 tggtggttaa atgtaaccca ggcaagggtt taatactcac cagtgtggcg ataaattctt
     2881 ttgccttaat cgcatcatgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg
     2941 ctttaccgcc acagcgtcgc cattcgaggc gtggtaaaag taaattgccc tgcgacgggt
     3001 caaacgcatt tagcccccag atgcgtaagt cggcgtgttc cgggtgctga cgaagaaacg
     3061 tttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca gcttcttcat
     3121 caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc agccagctca
     3181 aggcatcaaa agcgtctttg agagggaaag gaatatcaat aacccgaatg ccgggtgtcg
     3241 ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca
     3301 cgcggacctc tccctgttaa aagcctcaca ttatacgggg tacgacaaaa aaaatgcagt
     3361 accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg
     3421 ccgcgcccac accaattcct tgccagggtt tttcgtggac gtagtcatca gcacgataaa
     3481 cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt tttttcacat
     3541 catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg gcgggatcgc
     3601 cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtca tcatcaatac
     3661 gtgtatcacc aaactgatta gacattctcg ttctccattt gtgtaaaacc tgtcactaac
     3721 tatagacaag ggttattgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga
     3781 taccatcttc ttcataaata tcggtcacag ggatgaaacc aaattgccca taaaagtttt
     3841 gtaaatgcgc ctgcgccccc agataaatcg gcttctccgg ccagcggcga gtacacgatt
     3901 ccagcgcctt actcatcaat tgctgaccga ttttttcacc gcgtaaggct tcgctgacaa
     3961 tcacccgacc aatcacaacg ggttgcagtt cgtcgtcact tttcagaatc ctcgcatacg
     4021 ccaccagtgt cccgttatgc caaccgagaa tgtggcgatt ttctccctcc agatcgtcac
     4081 catcgatatc ctggtaagga cagttttgtt ccactacaaa taccgcacaa cgcagttgta
     4141 ataaggcata aagctgagaa acggataaat cagaatgatg cagatcttgc cagtcgatca
     4201 tggaaggctc ctttgtgctg tttgctttat actagccgga atattttcag cagcgtgatg
     4261 aactcatgga attaattttt ttaggtactt cagccggtgt gccaacccgt acgcgcaatg
     4321 tcacggcaat attacttaac ctgcaacatc cgacccagag cggtctttgg ttgtttgact
     4381 gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga aaactggaca
     4441 agatttttat cagtcacctt catggcgatc atctctttgg tttacccggc ttgctgtgca
     4501 gtcgttctat gtcaggcatt atccaaccct taacgattta tggtccgcac ggtatccgtg
     4561 aatttgtgga gaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg
     4621 tcgaaattgg cgctggcgaa attttcgatg atggcctgcg caaagtgacc gcttatccgc
     4681 tggaacaccc actggaatgc tatggctatc gtattgaaga acatgataaa ccgggtgcat
     4741 taaatgctca ggcattaaaa gctgctggcg tgccgcctgg cccactgttt caggcattaa
     4801 aagcgggcaa aacaatcacg ctggaagacg gaaggcagat taacggcgca gattacttag
     4861 ccgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcaatgccg
     4921 cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca
     4981 tggaagccaa agccaatagt cgcggtcata gctctacacg ccaggctgcg acactggccc
     5041 gtgaggctgg agtcggtaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag
     5101 ggtgtcagca cctgttacgt gaatgcaggt caattttccc ggtgactgaa ctagcgaatg
     5161 atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggcga aacttgccct
     5221 gttatcgcaa cccgcgctac atatatccac ccacacgggt atattgagcg atttttgata
     5281 tttattcatc tcctcacgca ctatattcag agcaataagt tgcctctttt tatcttcatt
     5341 cagcacaacc tttgcaacta aaaggagcct atgatggtta cagttgtcag caattattgt
     5401 caattatctc aaacccaact ctgtcagaca tttgcagaaa aatttactgt gaccgaggaa
     5461 ttactgcagt ctttaaaaaa acacttataa ctcattacaa ataatgatga aataacgctt
     5521 tttattgata atttacatat gaaaaaaatg acatctcttt gttttatacc attactacta
     5581 tcgcttactt ttattatttt ttaccaaccc tgggtcaggg cattaccacc aacgccccga
     5641 cacgctagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca
     5701 cgtagcgccg acaatattga taatctgaat aggtccgcag agtatataaa agaggtcttt
     5761 atcagtagcg gtgccagagt taccgcgcag gatgtcccca ttacgggtgg cccctacaaa
     5821 aacattgttg ctgattatgg tcctgccgat ggaccgctga ttattattgg tgcgcactat
     5881 gactctgtca gtagttatga aaacgatcaa ttgacctata cgccgggcgc ggatgataac
     5941 gccagcggcg tggcaggatt actcgaactg gcgcgtttgt tacagctgca gactccaggg
     6001 attggcgtcc aactggtcgc ctatgcgtcg gaagaaccgc ccttctttcg tagcgatgaa
     6061 atggggagcg cggtacatgc agcttcgctt gagcgtccag tgaaattaat gatagcgctg
     6121 gagatgattg gttattacga ctcaacgcct ggtagccagg attacccatc cccggcaatg
     6181 tcctggcttt atcccgatcg gggagacttt attgccgtgg tcggcagaat gcaggatatc
     6241 aacgccgttc gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct
     6301 atgaatgccc caggttttat tcccggtatt gatttctctg atcaccttaa ttattggcaa
     6361 cacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac
     6421 ttgcccggtg atatcgcaga cagattaaat tatcagaaaa tggctcaggt agtagatgga
     6481 gttacaacct tgttatacaa cagtaaataa taaaaatttc tcgaggcgga tccagaaaaa
     6541 gcaacatgcg cattttattt tctttcgtct gttttaagat gcgaaacatg aaattcaacg
     6601 gaggaaaaat aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac
     6661 caactgcttg agtctactaa aaaacctttg atggataata tacatgacac gctttcagga
     6721 ttacgccgac tggatattga taaacgctgg gattttttac actttggcct gacgggtaca
     6781 tctgcatttg accccgcgaa gaatgacccg ctaagtcgcg cagtgcttgg tgaacacagc
     6841 ctagaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
     6901 atcgaccgac tggaagctct ggatcgaagt gagctgcgta aacagttttc cattaagcga
     6961 ctcaatgaaa tggagattta tccgggagta accttttcag aagagttaga ggggcaactc
     7021 tttgcttcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga
     7081 caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagggcac caaaaaaaac
     7141 cgccgaacat gtcggcggct ttctgactta caaactgact gcttacatca gcggcattgc
     7201 taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc
     7261 agagatcagc accacgatac cgcctgcgct gtactgccag tttgatggtg catcgcgacc
     7321 cggttgttcc ggagcgtgaa ggtataagct caccgccacg cgcaggtagt agtagaggcc
     7381 gattgccgaa ccgacaacta cggcacccac cagccaccac aagtgtgcct ggacaccgac
     7441 cgccagcacg tagaacttac cgataaagcc cagcgtcatt gggataccag ccagtgaaag
     7501 catcatcacc gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga
     7561 gaacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa
     7621 cgcgccgagg ctgctgaaca gataacctgc caggtaaacc cctaccgctt ccatcgacat
     7681 ctcgccagtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga
     7741 gtaaccgagc agacgtttga tattggtctg gctcagcgcc atcaggttac cgaagatgat
     7801 ggaggcaaag gcgataatcg ccagcaccac gcgaatcgct tcgctgtcac ccaccggtgc
     7861 gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa
     7921 agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac
     7981 cagagagagt ttgaagccga ggccgacaat catcaggccg aaacctgcca gcaacagcgg
     8041 ctcgttgagc ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga
     8101 ctgcgcatac accagcgcca taccgaacag caggaaagaa gacgctgcgg cagagagaat
     8161 ggtgtatttg atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc
     8221 aaacagcggc aaagagatca gttcgatacc gaggaacaga gacgccagat ggttggcatt
     8281 cgccagcagg atcccgccca gggcggcaat taacaccaac aggtagaact cgtccttgtt
     8341 gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa
     8401 taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagtggcg taacgtccat
     8461 agcgcccgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc caataaccga
     8521 gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca ccaccaccac
     8581 cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat
     8641 ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat
     8701 cgcggagtgc gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac
     8761 caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc
     8821 aatctggctt ttcgctttac cgaagtaagc gcgatgtaac atcgccagcg aataaacaga
     8881 tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc
     8941 gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac
     9001 cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat
     9061 gcgcatgtcg cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc
     9121 cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc
     9181 agtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg
     9241 tttgatatcg gtctgggcga aggccatcca cgcaccgtag aagatgccga taacacccag
     9301 ccacatagcg attggcgcga actctgccga cgcgttcggg aacaacggta gggagaaacg
     9361 cagcagaccg taagcggcgg ttttcagcaa gatccccgcg aggtcaacgg aaccggcggt
     9421 tggagcctgt gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttcac
     9481 cgcaaatgcg atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt
     9541 attcaacagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac
     9601 cagcgccagg atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt
     9661 ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag
     9721 gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc
     9781 aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg
     9841 atatttttcg atctctttcc acgaacagag taccgccagc acaccgagca gaccggtcag
     9901 cacgaccatc agcagcgaca gcccgtcaat ggcgagatgg atagagatac caaaacgcgg
     9961 gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga
    10021 ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag
    10081 cgcgatccag cgcggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc
    10141 gataaaggga attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag
    10201 tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg
    10261 gtagtgtcgg atgcaccgcg aatgcggctt atccgaccta cgttcttata tgcccagggg
    10321 caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac
    10381 cgatgctcat ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt
    10441 tacctgcaaa gcgggaaagg acagccggga tgttcatcat tgagttcagc ggatcgcgtt
    10501 tcagcaacca ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa
    10561 agccccaggc gttgtaccac caggtaccca gcagacggcc cggcgcactg ttggcgatgg
    10621 aggtcaccag agtacgttta cccagccaca gccaggcagc cagcagaatg ccgaccaccg
    10681 cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt
    10741 gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca
    10801 gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt
    10861 cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac
    10921 cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct
    10981 catccttact gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca
    11041 ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt
    11101 tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa
    11161 agaacgcgtg ggtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga
    11221 ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg atatcggtct
    11281 gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaatg cccaccagat
    11341 gcagaacttc cggcgtcatc aggaacaggc cgtgggtacg agcgatcagg tagacacccg
    11401 cggttaccat cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt
    11461 cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca
    11521 tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca
    11581 ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagc gcgaaagcga
    11641 ggaacacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg
    11701 gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga
    11761 ggtacatcaa cagtaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt
    11821 tggtgtaagc gaagaagcga gagtagccct cttctccgcg catataccag gaggcataca
    11881 tgtgaataag gaaacccaca ccagtgacca ctgagagcat ggtcagcgac aggccgtcca
    11941 gcaccagatt gaaaccaatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc
    12001 tgtatgcctg ctcaccgtta gcgaagaagt caacgccgat aaaggcggtt accagcgccg
    12061 ccaggcccac agagccaacg ccgacgatcg ccgagacgtt ttcagaccag cgcccacggg
    12121 agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca
    12181 tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag
    12241 cagcagcgca aggccgatac tcgcttccgc cgccgcgagg ctgatggcga gaatgtacat
    12301 cacctgaccg tcggtctggc cccagtagct tccggcgacc acaaaggcca gcgcagaggc
    12361 gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc
    12421 ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat
    12481 gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc
    12541 gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa
    12601 gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg
    12661 attggcgtac cgtcgatacc ctgatcatta acaccaagga tggcgtaaac aatcaccacc
    12721 agcatgatgg ccgacaaaat cgccggacca atccacacct gcggtttcag ccactggcgt
    12781 tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc
    12841 ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcccagtgag
    12901 aagaacaccc cagaaatcgc cagcagcgaa ataatcaggt acagcagcgc gtgtaccgga
    12961 ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa
    13021 gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt
    13081 cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt
    13141 tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat
    13201 cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac
    13261 aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg
    13321 gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg
    13381 ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccggatcg cgggtcagaa
    13441 cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt
    13501 cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac
    13561 ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat
    13621 gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa
    13681 ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat
    13741 cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca
    13801 gccaccgaag aacagcgtca ccatcagtgc agagatggtc acaatcccga tgtattcgcc
    13861 cacgaagaac agaccgaact tcatgccgga atattcaatg tggtaaccat ccgccagttc
    13921 ctgctcggct tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc
    13981 aaaggtaata aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac
    14041 gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag
    14101 gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt
    14161 gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagaccgg ccatcatcag
    14221 gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat
    14281 cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg
    14341 atccgagaat ttcgggatcc agtcttcttt aaagaacatc ttgatcatgt ccgcgaccag
    14401 ctggagcgaa ccgccccagc caacacggtt aggtccgtaa cggttctgga acagacccag
    14461 caggcgacgt tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatcac
    14521 caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt
    14581 tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata
    14641 cccatcggca agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg
    14701 accgtgttgc catcgtaact aaaggagacg cgtgtacccg cgttcacacc caacttcgcg
    14761 gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca
    14821 cgctgtgaca attcatcgct accaaacagg tggtagtacg gcgcgatacg ccatttcccg
    14881 tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt ttcgctggtt
    14941 tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat
    15001 ttgttccacg cctgcgggga gttccagcct ggcgcccagg caaacggcac ttgcgaacgg
    15061 tgcgcggtcg gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc
    15121 ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg
    15181 tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct
    15241 ttgatacccg ccagttccgg gattttcgcc acaacagcgt caatcacatg gtcgagctgc
    15301 gtccagtcca cttcacggct cagcaaggtg ctgtgcagcg agtgtaacca acgccagctt
    15361 tccagcatga cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg
    15421 gcacggcctt cgttgttaat caccgtaccg tcgctttcag caaagctggc agcagaaagt
    15481 accagatggg cgttttccat aatcgctgtg cgttgatggt caaccaccat caccagcggc
    15541 gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag atcgttttcc
    15601 agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgat
    15661 ccaccgccca taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgataccg
    15721 acgtcagcgc cgcgaccttt cagggctttc gccacgttag ccgcagcctg aatcacttca
    15781 gcgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc
    15841 acgatgacgt cgattttgct ttgcagctca ggttcaatac cgtcaaccgc tggcgcggag
    15901 ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg
    15961 taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga
    16021 tgcttcgcac gttggccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt
    16081 gctgccgcca tttcgcgcgc tttacctttc accgcctgac gaactgccag cgcgacgcgc
    16141 gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg
    16201 cgcagtgccg gcgtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt
    16261 tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag ctcacgcagc
    16321 gcaaagttgc tttccacgct ggcacgcgga gaaccaatgc cgatcacttt cttcgactga
    16381 cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg
    16441 ccacgacgct gtactggctg acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa
    16501 cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga
    16561 cgcagttcgc cgtaacgctc gccggggctg atgttacagc cgatggaaca ttgctggcag
    16621 atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg
    16681 gtgaatacgc cggtcgggca aatctcgacc agattaccgg aaaattcgct ttccagcgtg
    16741 ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc caggtccgtg
    16801 ccgtcagcgt aatctttgta gtaacgcacg cagcggtagc aggcgatgca gcggttcatt
    16861 tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag
    16921 cgacggaagc tgtgtccagt catcacagtc atatcctgaa gatggcagtt accgccctct
    16981 tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg
    17041 aactgtttcg cttcttcatc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata
    17101 caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac
    17161 tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg
    17221 cccagagaca gacaagcttc cagcaagttg tccgctccgt tgacctcgta ttctttgccg
    17281 tctacatgaa ttgtagccat tagcatgctt ccagttttct cagtcagaga ctgagcgtta
    17341 atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg
    17401 ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc
    17461 gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa
    17521 gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tgaccttcgc cacgctccag
    17581 cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccaca
    17641 ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg
    17701 gtcaaccgcc atcgccagcg ccgtacccag acggcttccc gctttaccga tactttcgaa
    17761 ttccatcggc agatcaaggt gcgcttcggt caggaagtcg gtgccagcgc cgcctggctg
    17821 ccaggctttg aatttcagac cgtcacgcat accaccggcg taatcttcga ggatctcgcg
    17881 tgcggtggtg ccgaatggca gttcccacag gcccggattt ttcacccgac cggagaagcc
    17941 catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt
    18001 agcgaggatc gccggaacgt tacacagggt ttcgacgtta ttgacacagg tcggtttgcc
    18061 ccatgcaccg gaggttgccg ggaatggagg cttcgagcga gggttagcgc gacgcccttc
    18121 cagggagttg attaacgctg tttcttcacc gcagatgtag cgcccagccc cggtatggac
    18181 gaacaattcg aagtcgaaac ctgttcccat aatgtttttg ccaagcagac ccacttcggt
    18241 ggcttcggca atggcacggc gcagattcac tgccgcttcg atatactcgc cacgcaggaa
    18301 gatgtagcca cggtacgctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag
    18361 gtgcggcagt tgttccatca gcaggcggtc tttataggtg cccggctcca tttcatcggc
    18421 gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccatttcag
    18481 gccggtagaa aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac
    18541 gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcg ccttcgtaac cgtttttgct
    18601 gcgatattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg
    18661 cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct
    18721 tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttgtcgcag
    18781 ttccccaggc agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt
    18841 ttgatgttca gctttttctc cagcgccgcc tgaataccct ggtaaccgtt gatgtgacag
    18901 accacgctgt cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctggcta
    18961 tagaacgttg ccacgccttc gacgtcgctc gccggaatac ccagcacatc ggcgatcgcg
    19021 tggatcgcgc catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg
    19081 gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc
    19141 gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca
    19201 catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc
    19261 cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtacgg atacgggtgc
    19321 gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct
    19381 caatcatctg gaaagattca ttggctggca tcaccggacc ccacgacact tgcaggaagt
    19441 gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg
    19501 gatgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca
    19561 gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac
    19621 cacccaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca
    19681 cgtcgaagtc gatcccggtg gcgcgcaggc ccgcgccagt ggtgccccac tccagcgcct
    19741 ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggtg ttttgcagcg
    19801 ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcaggc
    19861 gatcccagcc acgcggcagg tcgtgcgcta caccgccaat acggaaccac gccgggtgca
    19921 tacgaaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga
    19981 agaacactgg cgtcatcgcg ccgacgtcct gaataaaggt ggagatatac agcaggtgac
    20041 tgttgatgcg gaacagttcg gagagcatta cgcgaatgac gttaacgcga tccggcacgg
    20101 tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga
    20161 ggtattcgat acggtcggta tacggaatgt agctgtgcca ggactggcgt tcgcccattt
    20221 tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatt tcttcgccat
    20281 caagctgcaa aacgatgcgg aaagccccgt gcgccgacgg gtggttcggg ccgaggttga
    20341 ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg
    20401 tcagggcttc catctccagg tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg
    20461 tagcgcgcgc cggataatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga
    20521 tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct
    20581 cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg
    20641 ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa
    20701 cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt
    20761 gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg cccacttcca
    20821 gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa
    20881 aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca agatgatcgc
    20941 gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg
    21001 gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa
    21061 aaattccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg
    21121 caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct
    21181 gatcgccaac cacccaggag agcggacgac gttctttacc gatagattcc tgcaacagca
    21241 tcagtgcctg catgtacgct tcaggacgcg gagggcagcc cgggatatac acatcaaccg
    21301 ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacataccg ccggagttgg
    21361 cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa
    21421 taaccggtgc catttttgta aagcaggttc ctgcaacgac catcaggtca gcctgacgcg
    21481 gcgaagctcg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag
    21541 tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac
    21601 gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttatta acttcttgct
    21661 ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg
    21721 gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag
    21781 cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca
    21841 gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag
    21901 cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca
    21961 gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc
    22021 gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggaacgttt ttcgaccttg
    22081 cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg
    22141 caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca
    22201 tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac
    22261 gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt
    22321 atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa
    22381 agtttcttca cattagttta cataatatca acaccattag catttaatgc cctttcaccc
    22441 cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa
    22501 tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga
    22561 gtattttctt gatctgtgac acgcttttgt cattccataa caaaaacgca gcaacaaatt
    22621 tacgtatttt ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc
    22681 gggacgtgaa tctctggtga ttgaaaaatg aacagttttg tacgttctgc acgatacgag
    22741 gaaggctttt tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt
    22801 acactattgt ctcagaaatt atctatggtc cgtcgattac tcgatatccc tttcaatcaa
    22861 caatgagtca tcgccttccg gagcagacat tgggctgtat tgccacggat tatggtagct
    22921 ctccatcgcc tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag
    22981 caaatattcg gtatcgggta gcggcggtaa gccatctact ccactcagca cacgcaggtc
    23041 tggactcatc atctcgaccg gccttgccgt cacgccaaga ccggctttta ctgccgcacg
    23101 aactgccgga agcgtcgagg cgacataagc cagtcgccat ggaatatctg ctttattaag
    23161 cgtcgccagc accatatcgc gaaacgggct aggatcatcc agcaatacaa gagggatcgg
    23221 ctcgcctttt tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag
    23281 gttcaatgcc ttaaacgttg aaggacgatg ggtggttacc atcaaatcca cttcctggga
    23341 ttccagcatt tctgccatat aggcattacg tttaacgcgg acatccagcg ctagtttcgg
    23401 ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga
    23461 agcaccgata gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc
    23521 attaaaacgc agaatttttc tggcgtaacc aagaagttga atgccatgtt cagttaacag
    23581 tttgttgcga ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat
    23641 ttgctgactt acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt
    23701 cagatcggca acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg
    23761 atttgcactt atcatatatt atcacttact ggcggctcat actgagctgg ttaatgctgt
    23821 gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc atccggaacc
    23881 cggacgaaag taaaaatgca tatgagttgc actaaaaaag cgactcacat tgttccgtta
    23941 taatgcctga agtagatcac agaatatatc ttcaggaatc gcatatctat taagttactc
    24001 actcttttgt atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc
    24061 actctcgtgc agcatcgctg gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc
    24121 atcaagagca gggatccgtc atttatgcga gcattttacc ccaaaaacct tttatttata
    24181 agggtcattg cgaattatct gatgcaaagt tatgttatgt taggcaaagt aatcttctat
    24241 tattaataag cacaacaaaa cctttttgaa tattaa
//
LOCUS       DAZGTJ010000083        23477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000083 DAZGTJ010000000
VERSION     DAZGTJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.083"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(70..978)
                     /gene="melR"
                     /locus_tag="ACHK8E_003816"
     CDS             complement(70..978)
                     /gene="melR"
                     /locus_tag="ACHK8E_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP0346027.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1261..2616
                     /gene="melA"
                     /locus_tag="ACHK8E_003817"
     CDS             1261..2616
                     /gene="melA"
                     /locus_tag="ACHK8E_003817"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP0346028.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RQEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(2613..3263)
                     /gene="yjdF"
                     /locus_tag="ACHK8E_003818"
     CDS             complement(2613..3263)
                     /gene="yjdF"
                     /locus_tag="ACHK8E_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP0346029.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(3386..5032)
                     /gene="fumB"
                     /locus_tag="ACHK8E_003819"
     CDS             complement(3386..5032)
                     /gene="fumB"
                     /locus_tag="ACHK8E_003819"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0346030.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(5110..6450)
                     /gene="dcuB"
                     /locus_tag="ACHK8E_003820"
     CDS             complement(5110..6450)
                     /gene="dcuB"
                     /locus_tag="ACHK8E_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP0346031.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(7021..7740)
                     /gene="dcuR"
                     /locus_tag="ACHK8E_003821"
     CDS             complement(7021..7740)
                     /gene="dcuR"
                     /locus_tag="ACHK8E_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP0346032.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(7737..9368)
                     /gene="dcuS"
                     /locus_tag="ACHK8E_003822"
     CDS             complement(7737..9368)
                     /gene="dcuS"
                     /locus_tag="ACHK8E_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP0346033.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            9548..9778
                     /gene="yjdI"
                     /locus_tag="ACHK8E_003823"
     CDS             9548..9778
                     /gene="yjdI"
                     /locus_tag="ACHK8E_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP0346034.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            9790..10062
                     /gene="yjdJ"
                     /locus_tag="ACHK8E_003824"
     CDS             9790..10062
                     /gene="yjdJ"
                     /locus_tag="ACHK8E_003824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0346035.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            10289..10585
                     /gene="ghoS"
                     /locus_tag="ACHK8E_003825"
     CDS             10289..10585
                     /gene="ghoS"
                     /locus_tag="ACHK8E_003825"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0346036.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            10613..10786
                     /gene="ghoT"
                     /locus_tag="ACHK8E_003826"
     CDS             10613..10786
                     /gene="ghoT"
                     /locus_tag="ACHK8E_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP0346037.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(10905..12422)
                     /gene="lysS"
                     /locus_tag="ACHK8E_003827"
     CDS             complement(10905..12422)
                     /gene="lysS"
                     /locus_tag="ACHK8E_003827"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0346038.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(12659..14116)
                     /gene="dtpC"
                     /locus_tag="ACHK8E_003828"
     CDS             complement(12659..14116)
                     /gene="dtpC"
                     /locus_tag="ACHK8E_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP0346039.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(14175..16322)
                     /gene="cadA"
                     /locus_tag="ACHK8E_003829"
     CDS             complement(14175..16322)
                     /gene="cadA"
                     /locus_tag="ACHK8E_003829"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP0346040.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(16402..17736)
                     /gene="cadB"
                     /locus_tag="ACHK8E_003830"
     CDS             complement(16402..17736)
                     /gene="cadB"
                     /locus_tag="ACHK8E_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP0346041.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTAGTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAGSGAPFAISASTILGNWAAPMVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(18102..19640)
                     /gene="cadC"
                     /locus_tag="ACHK8E_003831"
     CDS             complement(18102..19640)
                     /gene="cadC"
                     /locus_tag="ACHK8E_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP0346042.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(19900..20011)
                     /locus_tag="ACHK8E_003832"
                     /pseudo
     CDS             complement(19900..20011)
                     /locus_tag="ACHK8E_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(20451..21293)
                     /locus_tag="ACHK8E_003833"
     CDS             complement(20451..21293)
                     /locus_tag="ACHK8E_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0346043.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(21378..21575)
                     /locus_tag="ACHK8E_003834"
     CDS             complement(21378..21575)
                     /locus_tag="ACHK8E_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0346044.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(21587..21826)
                     /locus_tag="ACHK8E_003835"
                     /pseudo
     CDS             complement(21587..21826)
                     /locus_tag="ACHK8E_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            21893..23055
                     /locus_tag="ACHK8E_003836"
     CDS             join(21893..22154,22154..23055)
                     /locus_tag="ACHK8E_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFP0346045.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT         6152 a         5568 c         5705 g         6052 t
ORIGIN      
        1 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
       61 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
      121 cggtgacatg ccgacatatt tgccgaacgt gctgtaaaaa cggctactcg aacgaaagcc
      181 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
      241 gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat
      301 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagtgc
      361 ctgatcatag ttttcggcaa taaagcccag catctggctg acataaaatt gcgcatggcg
      421 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga
      481 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
      541 gttcggactg ttcaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt
      601 cgccagtgat ttgatcacca tgccgtgagt aacgtggtta atcaggtctt tatccagcgg
      661 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
      721 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
      781 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
      841 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg
      901 ctggtattcc gaatatagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat
      961 aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta tggcaggagt
     1021 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccacag cggcgagggg
     1081 agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt cccataaact
     1141 cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagacg
     1201 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     1261 atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     1321 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     1381 attgatccca cccgccagga agagtcgcat attgtggtgc gcaagctgat ggattcagca
     1441 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat
     1501 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     1561 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt
     1621 atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc
     1681 tgccccgatg ccaccatgct caactacgtt aacccaatgg cgatgaatac ctgggcgatg
     1741 tatgcccgct atccgcatat caaacaggta gggctgtgcc attcggtgca gggaacggcg
     1801 gaagagctgg cgcgtgacct caatatcgac ccggctacgc tgcgttaccg ttgcgcaggt
     1861 atcaaccata tggcgtttta cctggaactg gagcgcaaaa ccgccgacgg cagttacgtg
     1921 aatctctacc cggaactgct ggcggcttat gaagcagggc aagcaccgaa gccgaatatt
     1981 catggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac
     2041 ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt
     2101 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     2161 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     2221 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     2281 agtgtgattt acggcaacgt ccgtaacgat ggattgattg ataacctgcc acaaggatgt
     2341 tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa agtcggtacg
     2401 ttaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgacc
     2461 gaagccattc ttacggaaaa tcgcgaccgt gtttaccacg ctgcgatgat ggacccgcat
     2521 actgccgccg tgctgggcat tgatgaaata tatgctcttg ttgacgacct gattgccgcc
     2581 cacggcgact ggctgccagg ctggttgcac cgttaagggg taatcgcttc cggcgctctg
     2641 gtgattaagc caaagcgccg tagctggcgg caatgaaagc gagcgaggaa tatcaccgtc
     2701 gttaatgcgc caagcagcgc gcagaacata tcggattgcg tgtcccactg gtcgccctgg
     2761 gtgccgagaa aatcatccgc tccctgaccc attgccagcg ccgcccacca ctcgattaat
     2821 tcatacatcg cgcttatcgc cagcgccacg cagcagacca gaaacgccac catcttacgt
     2881 ccgcgaacgt acatcccgcg tacaaggatc tctcgtgcca ccagtgcagg caccagcccc
     2941 tggaaaaaat gccccagttt gtcatacgga ttacggctca accccagcca ttcctgcacc
     3001 tcaaaaccaa cggggacttt cgcgtaggtg tattgcccgc cgaccatcag gatgatggcg
     3061 tgaaagaaaa tgagcgtata aagcagcgga gttaacggat aacgtttagc ggtggcaagc
     3121 agtagcggca cgacaataat caccggtgtc acttccatca gccaggtgag tttgtcatgg
     3181 gccgaaatgc cggtataaat caggattaac gttagcgcca gtgcgccggt gttaagaatt
     3241 aacggcttga gtgtgcgggt catggtgagt tcgcaagtca gggaaaatac tgactattga
     3301 ccagtaagcg tgaaattaca atcgcaaaag aggtggccca gggggatcac ctggcagcat
     3361 gctgccaggc gctgggccga agaggttact tagtgcagtt cgcgcactgt ttgttgacga
     3421 tttgctggaa gaagtcgttg cctttgtcat cgaccaggat aaacgccggg aaatcttcta
     3481 cttcgatttt ccagatagct tccataccca gctccggata agcgacgcat tccagatgct
     3541 tgatgctctg ctgcgccagt accgccgccg gaccgccgat actaccgagg tagaagccgc
     3601 cgtgtttatg acacgcgtcg gtaacctgct gactgcggtt acctttcgcc agcatgatca
     3661 tgctgccgcc gtgggattgc agcagatcca cgtaggagtc catacgacct gcggtggttg
     3721 ggccaagtga acctgatgga taaccggcag gggttttcgc cggacccgcg tagtagatcg
     3781 ggtgatcttt gatgtactgc ggcagttctt taccggcgtc aatcagctct ttcagcttgg
     3841 cgtgtgcaat atctcgaccc acaataatgg tgccggtgag cgacaaacgg gtggataccg
     3901 ggtattgcga aagctgggcg aggatctctt tcatcgggcg gttaaggtca actttcaccg
     3961 cttcgccttc accggcctgg cgcagttctt gtggaatgta ctggcctggg ttgtgttcca
     4021 gtttttcgat ccagatacct tcgcggttga ttttcgcttt aatgttacgg tctgcggagc
     4081 aggagacgcc catgcctacc gggcaggatg cgccgtgacg tggcagacgg ataacgcgaa
     4141 tgtcgtgcgc gaagtattta ccgccaaact gcgcgccaag accgagtttc tgggcctctt
     4201 ccagcagttc ctgttccagc tggacatcgc ggaacgcctg accatgttcg ttcccttccg
     4261 tcggcagttc atcgtaatag tgagcgcttg ctaacttgac ggttttcagg ttggtttccg
     4321 cagacgtacc gccaatcaca aacgcgatat ggtacggcgg gcaagctgcc gtaccgaggg
     4381 tacgcatttt ctcgacgagg aagtttttca gtttgccggg cgtcagcagg gctttggttt
     4441 cctggtagag atacgttttg ttggcagagc cgccgccttt agcgacacag aggaatttgt
     4501 actcatcgcc atctaccgcg tacaggtcga tttgcgcagg caggttcgtg ccggtgttga
     4561 cctctttata catgtccagc ggcgcgttct gtgaatagcg caggttatct tcgatatagg
     4621 tgttatagac gcctttcgac agcgcttctt catcaccgcc accggtccac acgcgctgac
     4681 cttttttacc gacgatgatc gcggtgccgg tatcctggca ggtcggcagc acgcctttgg
     4741 cggcgatttc ggagtttctt aagaattgca gcgccacgta cttgtcgttt tcgctggctt
     4801 ctggatcgtg aagaatagcc gcaacctgtt tctggtgtgc tgggcggagc atgaaagaag
     4861 cgtcgtgaaa ggcttgctgc gccagcaggg tcagggcttc tggttccact ttcaggatgg
     4921 tttcgccgtc gaagtcggca acgctaacgt aatcggaagt gagtagatag tattcggtat
     4981 tgtctttccc catcgggaaa ggtgcctggt agataaaggg tttgtttgac atagcttcca
     5041 gcctgtaact ctgtatttgt tattcgaaaa tggcgtgccg cgtagtcacg ggcggcacgc
     5101 aaagtgcatt tataagaacc cgtacatcgc ggcgaagatc cagccgaaga cgcacgatac
     5161 gctcacacca atcaaccccg gcagaataaa gctgtggttg atgacgaagc gaccgatgtg
     5221 ggtggtgccg gaacggtcaa actgaatcgc tgccagatcg ctcggataag tcggcaggat
     5281 gtaataaccg tagcaagccg gtgctgaagc cacgatgtat gccggatcaa cgccgatcgc
     5341 cagcgcgacc ggaacaatcg ccgccagcgc cgcagcctga gagtttacaa acttggaaac
     5401 cagcagcaga acaatggcat aggcccacgg atactctttc accatttcac ccagtacgcc
     5461 ctgaatttca gacatatgcg caccgaacat ggtttctgcc atccatgcga taccgtacac
     5521 cgccacgatg gcgatcatac cggaacggaa gacttcgttt tttgagatag acgcgggatt
     5581 ggttttggtc aggataataa tcagcgcccc ggtcagcagc ataaacatct gaataaccag
     5641 taccatcgac agcggtttgc cgccgaagga tggacgcagg tccgaatcag caccaagaag
     5701 ggcgactaca gcgattgccc cgaggaagat ccacattgcc agccagttgc ttttcggcag
     5761 ttttttatcc agcagcgtcg cggtatcacc gtaaacatac tcacggtttt ccggtacgga
     5821 gatgaatttc tggaactctt cgtctttatc cagatcttta ccgcggaacc agctgaagat
     5881 accgatcgcc aggataccga ttaacgtcga tggaatggtg attgccagca ggtcgaggaa
     5941 ctcaagatgg cgaccatcaa aggtgacatt acccagcatc gcaaccagag acacgactgc
     6001 aaccgacacc ggactggcga taatccccat ctgtgcaccg atagaacttg ccgccattgg
     6061 acgttccgga cggatgttgt tcttaatagc aacgtcgtag atgatcggca gaatggtgta
     6121 aaccacatga cccgtaccgc aaagaatggt cagcgtacag gtcacaaacg gcgcgacaat
     6181 tgagacatat ttcgggttgc ggcgcagcag cttctcggca atttgcagca tgacatcaag
     6241 accgcccgaa gcttgcaagg tcgccgatgc cgccaccacc gcaatgataa ccagcatgac
     6301 atcaaccggt ggtttacctg gctgaaggtg gaagacgaag accagaatga ccagaccgat
     6361 accgcctaat aaacccagcg cgataccacc ctttctggca ccataaaaca gacatatcag
     6421 tattatgata agttggatag taaataacat gtgtgaaccc tcgcgataat cctatttaaa
     6481 tttttgctga atagatcaca gtcacgttct gttttgtatg aactgttttc agagcctgac
     6541 taaatatccg tttggtcgtt accggcttta gcaaatacct cacagtgaat attggctgat
     6601 taaagcagcg gcaaatttat gcactgtgat aagcggcttt ttcagagaga aaaactctct
     6661 ggcgatgggt ttatagtttg tactgcgcag atgttaatgt tttgtttgct gttatcttat
     6721 tgatatttat cggtctattt tttgttctca gggcagatat cattaggttt atagattaat
     6781 ctgatctacc catttgtggg taaaaataca cattatgcgg gtgacataat agttaattaa
     6841 cttttgttag cgttttgaaa ttaaaaacac cgttcatctg aagagatatt aatttttaac
     6901 gatgatggag ggataattat atttgatctg gcacaagttt tactgatgaa gaatgtaact
     6961 tgtgccagga atattttgca ttacagtaat aattattact tacagataac cagcgatccg
     7021 ttattggcaa tattgtttca gcagtgagta gtgttctgcc tgaatacggt aacggtaaac
     7081 cggacgccct gtgacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca
     7141 gatgaggtat ttacggcagg aaacacgcga gatgttaacc tcgttggcca gctcgtcggt
     7201 tgaaaactca tagtcctgat gcgcgtcaat ccactggcac agcgtgcgta acgtctgcgg
     7261 cgtcaagcct tttggcaagc gacgaggatc ctgttcgttg gagctgctgc cgtggattag
     7321 ctgatcaagc tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg
     7381 ccagccggtg agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac
     7441 gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg agatgacaat
     7501 cacatcactt ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt
     7561 ttgcatatag atatcgagca atatcaggtc gataggcgta tcgctattga agataatctc
     7621 tttggctttc tccagcgtcg aagccgttcc acagcattga aagcctggga tttgtgccac
     7681 gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg ataattaata cattgatcat
     7741 ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt
     7801 tccgattcca cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa
     7861 ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgattttg
     7921 tcgggtgcga tccccggtcc atcatcgtta acttcacagt gcagccagcc gtgacggtag
     7981 tgcaatgtta cgctaatttc gccaccgggt tccggtccta atgcctccag cgcgttttct
     8041 atcagatttc ccagcgtggt aatcagcgtc gcgacctggt cttcactgcc gctgtctggc
     8101 aactggcttt cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg gttaatctta
     8161 ctgattaaaa aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct
     8221 tcttgatagt tattggctgt tttgagaatg taatcttcca actgcttata actcttcaga
     8281 tgcaataatc cgagaatcac gtgcaattta ttcataaatt cgtgggatcg ttcacgaagt
     8341 gcgtcagcat agttgaccag accatcgagt cgctgcatca gtttacgtac ttcagttttg
     8401 tccctgaagg ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg
     8461 atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc gccgcggggt accgtcgcgt
     8521 aacacttccg agacatctac cacctgtgac cacgagtggc ttagcgtcga cagcttctca
     8581 tcgtcctgcg acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgact
     8641 tcaccgcgat cgtccacggc aacgacgcct tctttgatgg actgcaacat agcctggcgt
     8701 tgctcaaaca gcgtggagat ttcgtagggt tccaggccga aaaggatttt tttcagtacc
     8761 ttaaccagaa tgcaggtgcc aatcagcccg accagcatgc caaataatac cgaccagatg
     8821 atgctccagc gactgtcatt gatctgttgg gtcacgcgac ttaactcaag gccgatcgcc
     8881 accacgccaa tttgtttatg attttcatcg tagatggggg taaatacgcg taaagcctgc
     8941 gccagaaaac cgcgattgat agcgacattt tcttcgccat tcagcgcttt aaggatgtca
     9001 tcacctttaa atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactttgc
     9061 atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc
     9121 tggattccac tctcctgtgg ttttttctgc aagccctgac ggatttccgg cgagtcggcg
     9181 agggtacgcg ctactgccag tgccttgtta gctagcccgt ctcgcgtcat atcactgatt
     9241 tgcgagaagt aaatcagatg caccaccaat agcaccgaga acagtaccgc gctgaccatt
     9301 aagatcactg tggtactcaa tttcatcgga cgtttgcgta acatgcggta gggcaatgaa
     9361 tgtctcatca gcttccttgt gtgacaaatt tcttaagcat tatctctgat gaggcgggta
     9421 attcaaaggg agtaagaatg attggcatat aggggaagag actctggcaa cggaaactgc
     9481 cagtgctgta tgaagattcc agggctatgc ttatagtgaa aaccatactg atgagagagg
     9541 gaaggtcatg gatcaggcgc tactggacag gggttatcgc tgttataccg gcgaaaagat
     9601 cgatgtctat ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg
     9661 caagttattt aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgccac
     9721 tgtggttaaa gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc
     9781 gagggtaaaa tggaaatacg cgaaggccac aataaatttt acattaatga cgaacagggc
     9841 aagcaaatcg ctgaaattgt ctttgtgccg accggagaga atttagcgat tatcgaacat
     9901 accgatgtcg atgaaagcct gaaagggcaa gggattggta aacagctggt tgcgaaagtc
     9961 gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac
    10021 gaatttgata aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca
    10081 cgatattttt catgctctcc gcggtgtgat gcaggagagc atctgaaggg tagggggatg
    10141 cacaaagaat gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa
    10201 aaaaagaata tctcctatat gagaatcatc aatcggggtt aataagtttt gcgtccccag
    10261 agcgtttaat attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt
    10321 tgtttctttt gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat
    10381 gtatgatatg agtttctcct ctatcgtggc tgatgaatat gggataccac gacaactgaa
    10441 tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag
    10501 gcttaaatgc ttagacttac cggatattga ctttgacctc aacattatga cagttgatga
    10561 ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact
    10621 attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg
    10681 gtttatctca catgagaaaa cacatattcg tttacttagt gcatttctgg tcggaataac
    10741 ctggccaatg agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag
    10801 tggacccgct ggttatcttg aaacgaagcg tctctcctca aaactggatt tgcggaatga
    10861 cgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg aaatttattt cagtgggcgc
    10921 atcgccggga agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt
    10981 cggtcgatac caatacccag accagcggtt ggcggcagac catattccag cgcagttacg
    11041 taatcttcgt catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc
    11101 tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accaatttca
    11161 cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc
    11221 gccagcggtg acacttctgc cggatattcc gtgataaagg ttggctgaat cagatgtgct
    11281 tctgccactt catcaaagat ctccgtgaca atgcgtccca acccccagct tttctctacc
    11341 gtaattccga tagattcagc taatgcttta gcagcatcaa aattatccag atccgccata
    11401 tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg
    11461 ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagtac ctcttgtgcc
    11521 agagtgcgga acagtgattc cgtcagttca atcaaatcgt ggtaatccgc atacgccatg
    11581 tagagttcca tcatcgtgaa ctcaggatta tgacgaacag aaataccttc attacggaag
    11641 ttacggttga tttcgaatac ccgttcaaaa ccacctacaa ccagacgttt cagatatagc
    11701 tccggcgcga tacgcaggta catatctaaa tccagagcgt tatgatgggt aataaacggg
    11761 cgagcagatg ctccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg
    11821 cgagcgacca tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga
    11881 cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc
    11941 tgcagaccat ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcagctca
    12001 gtacagtgaa tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata
    12061 atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct
    12121 cttgcaacgt acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc
    12181 cccataatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc
    12241 agttcctggt tatccttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg
    12301 cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga
    12361 tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc tcgttcagac
    12421 attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca
    12481 ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc
    12541 tcaagttgat atcactaata aataaccata aaaatggttt aaatgactta ttaatctcga
    12601 tttgctagtg tgataaactg aaagttaatt tattcataac caattgtttt tacgacagtt
    12661 aatcgttgct ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg gtagcaaagg
    12721 ccaataccac gatgatcgcg acacaagcca acgtccactc tcccatctga gaaaagaatc
    12781 gctggtaagc ggcaattgcc ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga
    12841 cgcctgccag ccagttggcg accgcgcctg ttgccagcat ataaataccg gttaatacgc
    12901 cagacatttt cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag agttcagcaa
    12961 agcccattag cgccagcccg gatatcatca cgcccattga cgcttgaccg ccagccgctg
    13021 catgtcgggc atcaaatgcc agcaacataa agccacaagc catcagcagt aggccaaagg
    13081 caaacttcag ccagacgcgc aatgctgaat tgccgcggct ttctggcgac gccagccagg
    13141 ccagaacaac cccagcgagc atcaccgcaa tagcattcac cgactggaat agtgctgtag
    13201 gtacttcaat attgaatgcc tgacgattca caaagcgatc gataaacaag ctgatggtac
    13261 tgccgccctg ttgtgccagt acccagaaca acgtcccgac aaacatcaac aatacaattt
    13321 gccaaagagc acggcggtgt tcggggaatt tgatcatcat gcgggcaatg atttgtgctg
    13381 caatgaggca aacgatcgcc agcaaatatc cggaccagtc gttctccagc agcagggtaa
    13441 aaaatactgg ggctaaacag agcatcacca ctaaccagct ccatactggt aaggcaaatt
    13501 tgacgctagt gagcgctttt ttatccatac tacgcgtgga ctggaaatga cgatgaccac
    13561 ttaagaaaat caacaaaccg ataaacatgc cgcctcccgc aagggcaaag ccaacatgcc
    13621 atccatacca ctgagcagcc aggccgcagg cgatgggcgc ggcgatagaa ccgatattgc
    13681 ccgcagcata tagcagcgaa aaaccgccat cacgtcgatg atcgttctcg tcgtagagct
    13741 cgccaagcaa acagctgatg tttgatttga ataaaccgta gccacaaata atgattgcca
    13801 gcgccagata caggctaaag gttgaatttg tatcaatgcc cagcaccaca tggccaaggg
    13861 tcattaacag cgcgccggca atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc
    13921 agccgccgag aataggggta acgtaaacca gagaagcata tgcgctgaac aggctgatgg
    13981 catggttatc atcaaaacca agctgatggg tgagatagag aatgagtaag gcacgcatgc
    14041 cgtaaaaact gaagtactcc cagatttgga tcgccacgat atagtatatc gcgcgcggct
    14101 gtgagggtgt tttcatgtgt tctccttatg agcaaaaaag ggaagtggca agccacttcc
    14161 cttgtacgag ctaattattt tttgctttct tctttcaata ccttaacggt atagcggcca
    14221 tcagcctgac ggtatgcacc gtgaatatcg gtttcaaagc ccggatagtg agcgccgatt
    14281 tcacacagca tctgcaggaa ctccagaacc ggacggcttt cttcggtgat catttcaccc
    14341 ggcattacca gaggaactcc cggcggatac ggaaggatca tattggcgtt aatacgacct
    14401 accatttcgt cgaggtaaac ttcttcggtc ataccgtgca gctctttctg gaacgccgca
    14461 tacggagtca taaccattgt cggcagaact tcaaatgcgc gatacatcag atccggcaga
    14521 ttgtggtgaa caatcagttt gtggatattc tgagccagtt cctgaatacg catatcttca
    14581 tagaattcag gatcttcacg atatagagac ggcagcatgt ttttcacacg caggttcagg
    14641 tcgaacgcac gtttgaagtc agtcagagca cgcagcaggc tcaatgcttt ggtcttatca
    14701 ataccgatgc tgaacaggaa cagcaggtta tacgggccgg ttttctcaac aacgatgcca
    14761 tgttcgtcga ggtatttcgc cacgatgctg gccggaatac caaagtcgct catggtgccg
    14821 tctttttcca tccccggggt cagcagggtg actttgatcg ggtcaagata catgtgctca
    14881 ttgtcgatgt tcttgaagcc gtgccaggag ctgtcagaac gcagcggcca gcattcagtg
    14941 gtatcgatat gatccggctg ccatacatca aagaaccagc catcagattc cgttctcaga
    15001 cgtttgatct ctttacggaa cttgatcgca cgttcaatgg aaccgttgat cagacgctta
    15061 ccagcattac ctttcatcat cgccgcagcg gtttcagtgg acgccacgat accgtagtgc
    15121 ggagaggtgg tggtgtgcat catgtaggct tcgttaaagg tttcttcgtt tacgtcacct
    15181 ttaacgtgga tcatggaagc ctgagagaac gccgccagca gtttgtgagt ggactgggtt
    15241 tcgtaaatca ctttcccttc tacacggcca ccgctcatac cgcatttacc ttcgtaaatc
    15301 ggcgagaagt tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagc
    15361 gttttcttga tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattacg
    15421 gcatgtaccg gccaggttgc gtttggtgtt tctttaacgc gcttagcaat ggttgcgtgc
    15481 tggaattcac tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa
    15541 tagattgggg taacatcgct catcatcatt aggtgggtta gcgatttgtg gcagttacgg
    15601 tcaatcagaa tggtgctgcc tgccggagca gagtacatac cgacaatttt gttcgcagtg
    15661 gaagtaccgt tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt
    15721 tccgcttctt tgtgtggacc gctgtgatcc agcagagaac ccaattcaga tactgaaatg
    15781 gaaatatcag atttcatggt gttcggacca aagaaatcat agaacaggct acctaccggg
    15841 cttttctgga atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca
    15901 cgaacatatt taaacagtgc tttggtcagc ggaggcagaa tagtgttgat atattcgtca
    15961 gtggtctgct tgatcttgtt agcaatatca tcagcagcac ccagcgcata ttcaaagaag
    16021 ctaatctgta aacgcagatc gttcaggctt acatcgagtg tggaatacgt attagcgaac
    16081 gcgtacaacg gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat
    16141 ttatcccagt caaaaataac accgcacaga cgcgcattgt tttcgatcag ttttaataag
    16201 tcgtcacggt cattcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt
    16261 tcacggatgg gttcttcttt aaaataaaca cccatgtgat tcaatattgc aataacgttc
    16321 atagtcatat ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg
    16381 agcctcggaa aatactttta attaatgtgc gttagatgct gtgtggttat ccattgagtg
    16441 gctctggcgc tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa
    16501 ggtaccagcc agctcgaagg agcttgcgcc catcagagcg atgaagcaga acacgcaacc
    16561 cagtacagag cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag
    16621 gtcaacgcaa gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc
    16681 accgaacagg tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag
    16741 ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt tgctgtcgac
    16801 ttcaccataa actttcggga agttaccgtc gttagcggca cgcacacctg cctggccgac
    16861 cagcatcatc caggagccca gagaagtcag gcacgcaaaa gcggtgaatg cagaaaccat
    16921 cggcgcagcc cagttaccca ggatagtaga agcactgatt gcaaacggag caccggagcc
    16981 tgccattaca gaagacggat acataccgga aagaacctga gtcgcagcaa tgtaaacaat
    17041 acctgctaaa ccagtcccca gcatggttgc cagtggaaca gtacgtttcg ggtttttaac
    17101 cataccagta cttacagctg cggattccac acccacgaag gcccacaggc agagcagaat
    17161 acttttaatg attgcatgac catcagtagt acctgcggta ttccagttag ctgcataagt
    17221 tgcagcatca aaccaatgcc agccaacaat agcagtcata accacaggaa taagaaccag
    17281 taccaggcca atagtggtta aacggcttac ccaggtaccg ccgagcatat ttacaaaggt
    17341 aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat ttaatactgg
    17401 gaagaaggtg gaaagataag atacagcggt aataccaatt gccaggttac caatccagtt
    17461 agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggagaaattt ctccggcata
    17521 cgcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata catatgccag
    17581 cgacattgca ccaataatag agataatcca accccagata gcaataccac cgatacttgc
    17641 taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa caacaccggt
    17701 acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat ttcatttttg
    17761 aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac tggcgctaag
    17821 attactcacg aaaaaaggat taatcctaaa gattaggtga aataacacaa aagtttctgt
    17881 aagtgagaac ttgaggtttt ttattaacac atcaggatcg caagttgata tcatgaaaag
    17941 ataaacattc aatgtttaca atgtgttatg tgacattttc tggttaaatt tatgtaataa
    18001 aaattatgcg gcaaataaat tgccgcaaca tattatacca acaggaacat acaaaaactc
    18061 aacaacaaat attcccgagc atgaatcaac ccgggagtta cttattctga agcgagaaat
    18121 ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt ttcaatccag
    18181 taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc agctgcttcc
    18241 cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag ccaggacatt
    18301 tcaagatcaa tgccagtatt tatcgcctga taagactcat ctgttttacc ttttaccagg
    18361 gcactgaccg cttttatttg atatataatg gacaggttgt tcaattccgg cagtgtaaca
    18421 atgttatcta tttctgtgtt aagtgctgct aattgttttt cgtctaaagg atgttgagaa
    18481 tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc tggggatgat
    18541 tgaacaatct cacctaataa ttcactggca cgatccaatg atttatcatc accatgcagt
    18601 aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgcgg caaaatttgc
    18661 tggagcatct cctgcattcg ttgtggccac ggttggttta acgcttttga taaactctcc
    18721 agtaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg tttatccagc
    18781 atggttgaac catctgcatt gtctaccaat tttaccgaca taaagcattg ttgagcacgg
    18841 tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct cggttcatca
    18901 atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag tgatgtcgcc
    18961 accaaatcac ccacgcctat cgcgtaagag agctgatacg gggaactcca gctgttacaa
    19021 cttttattta ccatattaat gtcaatatcg cgtggattga gcaaaatacg cgatttgctc
    19081 atcggaagac gtgtatcaag acttgaaaac gctaccagtg ctacacagat acctaacgac
    19141 aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac tggggattgt
    19201 tcaggtggcg ttgcggtgtt ttgaatgtta agactgtggg agggagaatc tgtggcagga
    19261 accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc ttcttcgctg
    19321 taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc gatatagaca
    19381 ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact ctgcgtcaca
    19441 acgtgattgg tgacaatact tctcttccag acattatcga taagttcatc cctgctaagt
    19501 acttcgccac tgtgttgagc aaagaaaacc agaaggtcga ttaatctcgg ctcaagggta
    19561 agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca ttcgccaacg
    19621 cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatggtg caatgataat
    19681 tggcataacc tatttttacc atctatagat gggtttattt acatattatt ggtgaatgca
    19741 agacgttatt tttaccagac ataaacttct tgattacata gtgttgagaa aggattttac
    19801 tgagaaccag aagtaatatt ccttaccatc aaaattcatc atctttgcca aagaaaaatg
    19861 ttcagaaaat aatccatgga aaattgtccg gagcgcttac tattttaatg gattgttagt
    19921 ctttgcatga gcaaacggac tgatacattt ctctttgttc tcattcagaa aatctcatca
    19981 gttggcgttc tgcccgatgt tgtgctttat gggtgcgtta cgctcctgat gacgtcattt
    20041 gacgttcaac agcatcacgg ggccgcacga catttcacgt cagtcagcgc tatagctcag
    20101 aaacaaattt tcccgaattg gaatatgccc gcaaattgct ggggatgtgg ggagaatctg
    20161 gttgagttcg gtagaattga tttggcggca ggacgcttaa atcgtggcgt cctgaaacga
    20221 aaaacggacc tccgtggagg tccgtttata tgaatttggt gccggactcg gaatcgaaga
    20281 tttcatttat catattgata ttaatctcgt ttatttggtt tgatgaaaaa tgtaccctct
    20341 cttgtaccct cggtcccttc tggtggtttt tatgagaaca agtagatcaa gatttttgtc
    20401 atcagaacac gtgtattttt gtgggtttta ttcctgagtt ttattccttt ttatatcgct
    20461 aacgctccgg gatagtgctt agcaataata tcattaagcc tgtcaaccag ctctggtttc
    20521 ctgaacgtga tgtgcgcgga gcccttctta aagtatctga tgctgaacat ctcatcttca
    20581 tagtcctctt taccctgaac agactggacg tgatcatcca gatgaatagt gatgttttcc
    20641 cggttgtcgg ggatcggttt gccgctgaac aggtgtagca ttatttccag atcagcaagt
    20701 cgatctgcct gttgcccggt gataagatga aagccccacc ggtcccacct caccaggttg
    20761 ttgacgataa ttttactgcc aaatttgcag gggcaattgg ttttgtaatt ccagctcaac
    20821 cctctgaaga cgttgatcac cccccgctca aacacctcat ccttattctg gtgcagttgt
    20881 tcaaatgtgc tcagaatgtt cgcttcactg atttccggaa aattatcgta ttccagtgac
    20941 cggtaccatg tatcacgggc ctgtgcatcc attaaggaaa gcatcccgga acgttgcatc
    21001 aggtcacgcc agatttcgcg atcgagattg cgtgtgatgg ctttcattgc ggtttcaggt
    21061 ttctccatca gccagcaacc gcagtgaaaa ttctgtttca tggcccagtc cagtgcggtt
    21121 ttaccgccaa tgctttctgt cattgctgag atttcagcca gttgccggat aagtgtgtcg
    21181 atttgcagca gtgtactctt tcgtacagcc agaatgtgga ggatactggt tgagcagata
    21241 acgtccgtat gccagttgac aacttccgga cgggagactg tgatatcaga cataatcgat
    21301 attccttttt gcagataaat gaaaacgcca gccctgttca ggctggcgtc agtggaatga
    21361 agtctggtga gtgagagtca ccggttctgg tgcagaagct gcaactggcg cagtgtacgg
    21421 atatcttccc tggcctgttc aatgcagggc agcagtgctg ccgaatccgt tttatcctca
    21481 tcgttgtcaa agataattcc cgattcgcag tcgatattgt cctgcagcca cgcaatcaga
    21541 atatccagcg ctgtttccgt ggttaatgat ttcatgttgt ttctggttac cagtcgaaag
    21601 tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg acgccataac
    21661 gtcgggtaag tgtgacaacc agacggcgga acgtttttga cagccccagg cgtttaagcc
    21721 gcagaaccgg gtatgaccac gcatcggtac gtaacagata tccgctacct gtgaaatgaa
    21781 tccattctga ttcgccggtg tcattcgtct gatgtgacag cgagtactga tcttacccag
    21841 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acatgacaaa
    21901 aacagtatca accagtaaaa aaccccgtaa acagcattcg cctgaatttc gcagtgaagc
    21961 cctgaagctt gctgaacgca tcggtgttac tgccgcagcc cgtgaactca gcctgtatga
    22021 atcacagctc tacaactggc gcagtaaaca gcaaaatcag cagacgtctt ctgaacgtga
    22081 actggagatg tctaccgaga ttgcacgtct caaacgccag ctggcagaac gggatgaaga
    22141 gctggctatc ctccaaaagg ccgcgacata cttcgcgaag cgcctgaaat gaagtatgtc
    22201 tttattgaaa aacatcaggc tgagttcagc atcaaagcaa tgtgccgcgt gctccgggtg
    22261 gcccgcagcg gctggtatac gtggtgtcag cggcggacaa ggataagcac gcgtcagcag
    22321 ttccgccaac actgcgacag cgttgtcctc gcggctttta cccggtcaaa acagcgttac
    22381 ggtgccccac gcctgacgga tgaactgcgt gctcagggtt acccctttaa cgtaaaaacc
    22441 gtggcggcaa gcctgcgccg tcagggactg agggcaaagg cctcccggaa gttcagcccg
    22501 gtcagctacc gcgcacacgg cctgcctgtg tcagaaaatc tgttggagca ggatttttac
    22561 gccagtggcc cgaaccagaa gtgggcagga gacatcacgt acttacgtac agatgaaggc
    22621 tggctgtatc tggcagtggt cattgacctg tggtcacgtg ccgttattgg ctggtcaatg
    22681 tcgccacgca tgacggcgca actggcctgc gatgccctgc agatggcgct gtggcggcgt
    22741 aagaggcccc ggaacgttat cgttcacacg gaccgtggag gccagtactg ttcagcagat
    22801 tatcaggcgc aactgaagcg gcataatctg cgtggaagta tgagcgcaaa aggttgctgc
    22861 tacgataatg cctgcgtgga aagcttcttt cattcgctga aagtggaatg tatccatgga
    22921 gaacacttta tcagccggga aataatgcgg gcaacggtgt ttaattatat cgaatgtgat
    22981 tacaatcggt ggcggcggca cagttggtgt ggcggcctca gtccggaaca atttgaaaac
    23041 aagaacctcg cttaggcctg tgtccatatt acgtgggtag gatcactact catactataa
    23101 gagggtccac ttccccccgg atatatgatg atggatggag tatccggagc ccagcatgct
    23161 ccattagcat caatatatgt tcccatatct tttccacgta gatctattac attagtagaa
    23221 ccaggtaatc cattaagatt catccctcct ttcacggaat caagcatgtt atctgatatt
    23281 tctctcattt taaaaacctc ctgctcattt atgttcagat attaatttgg tgttgcaacc
    23341 atttattatc acagaattca gaatatcatt cagttttatt tttgttaaaa tatcccctct
    23401 taagacagac acatattctt gtttatcatt ttcattaaac aatgactcta tattttctac
    23461 gtttaaataa tatagcc
//
LOCUS       DAZGTJ010000084        21785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000084 DAZGTJ010000000
VERSION     DAZGTJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.084"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..413)
                     /locus_tag="ACHK8E_003837"
     CDS             complement(<1..413)
                     /locus_tag="ACHK8E_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0346046.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSES"
     gene            complement(616..1017)
                     /gene="nikR"
                     /locus_tag="ACHK8E_003838"
     CDS             complement(616..1017)
                     /gene="nikR"
                     /locus_tag="ACHK8E_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP0346047.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1022..1828)
                     /gene="nikE"
                     /locus_tag="ACHK8E_003839"
     CDS             complement(1022..1828)
                     /gene="nikE"
                     /locus_tag="ACHK8E_003839"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP0346048.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1825..2589)
                     /gene="nikD"
                     /locus_tag="ACHK8E_003840"
     CDS             complement(1825..2589)
                     /gene="nikD"
                     /locus_tag="ACHK8E_003840"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP0346049.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2589..3422)
                     /gene="nikC"
                     /locus_tag="ACHK8E_003841"
     CDS             complement(2589..3422)
                     /gene="nikC"
                     /locus_tag="ACHK8E_003841"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP0346050.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3419..4363)
                     /gene="nikB"
                     /locus_tag="ACHK8E_003842"
     CDS             complement(3419..4363)
                     /gene="nikB"
                     /locus_tag="ACHK8E_003842"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP0346051.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4363..5937)
                     /gene="nikA"
                     /locus_tag="ACHK8E_003843"
     CDS             complement(4363..5937)
                     /gene="nikA"
                     /locus_tag="ACHK8E_003843"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0346052.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6048..6635)
                     /gene="acpT"
                     /locus_tag="ACHK8E_003844"
     CDS             complement(6048..6635)
                     /gene="acpT"
                     /locus_tag="ACHK8E_003844"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP0346053.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6690..7739)
                     /locus_tag="ACHK8E_003845"
     CDS             complement(6690..7739)
                     /locus_tag="ACHK8E_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0346054.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7871..9088
                     /locus_tag="ACHK8E_003846"
     CDS             7871..9088
                     /locus_tag="ACHK8E_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346055.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9092..9649)
                     /gene="dcrB"
                     /locus_tag="ACHK8E_003847"
     CDS             complement(9092..9649)
                     /gene="dcrB"
                     /locus_tag="ACHK8E_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP0346056.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(9722..10387)
                     /locus_tag="ACHK8E_003848"
     CDS             complement(9722..10387)
                     /locus_tag="ACHK8E_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP0346057.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10609..10854
                     /gene="tusA"
                     /locus_tag="ACHK8E_003849"
     CDS             10609..10854
                     /gene="tusA"
                     /locus_tag="ACHK8E_003849"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP0346058.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(10956..13154)
                     /gene="zntA"
                     /locus_tag="ACHK8E_003850"
     CDS             complement(10956..13154)
                     /gene="zntA"
                     /locus_tag="ACHK8E_003850"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP0346059.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13228..13854)
                     /locus_tag="ACHK8E_003851"
     CDS             complement(13228..13854)
                     /locus_tag="ACHK8E_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP0346060.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13995..14354
                     /locus_tag="ACHK8E_003852"
     CDS             13995..14354
                     /locus_tag="ACHK8E_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP0346061.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14357..14626)
                     /locus_tag="ACHK8E_003853"
     CDS             complement(14357..14626)
                     /locus_tag="ACHK8E_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP0346062.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14616..15212)
                     /gene="rsmD"
                     /locus_tag="ACHK8E_003854"
     CDS             complement(14616..15212)
                     /gene="rsmD"
                     /locus_tag="ACHK8E_003854"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP0346063.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15362..16858
                     /gene="ftsY"
                     /locus_tag="ACHK8E_003855"
     CDS             15362..16858
                     /gene="ftsY"
                     /locus_tag="ACHK8E_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP0346064.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16861..17529
                     /gene="ftsE"
                     /locus_tag="ACHK8E_003856"
     CDS             16861..17529
                     /gene="ftsE"
                     /locus_tag="ACHK8E_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP0346065.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17522..18580
                     /gene="ftsX"
                     /locus_tag="ACHK8E_003857"
     CDS             17522..18580
                     /gene="ftsX"
                     /locus_tag="ACHK8E_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP0346066.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18825..19679
                     /gene="rpoH"
                     /locus_tag="ACHK8E_003858"
     CDS             18825..19679
                     /gene="rpoH"
                     /locus_tag="ACHK8E_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP0346067.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19951..21054
                     /gene="livJ"
                     /locus_tag="ACHK8E_003859"
     CDS             19951..21054
                     /gene="livJ"
                     /locus_tag="ACHK8E_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP0346068.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21242..21625)
                     /gene="panM"
                     /locus_tag="ACHK8E_003860"
     CDS             complement(21242..21625)
                     /gene="panM"
                     /locus_tag="ACHK8E_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP0346069.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5189 a         5729 c         6029 g         4838 t
ORIGIN      
        1 gactcgctgc ggctgtaacc gtcctcaccg ggaaacaggt gctcaaaata caggcttctg
       61 ccgccgttat cactgagtat cagtgtgtta tcgcgcagct gtaagcggat atccgcaggc
      121 attttccagc cggggccgag gctccccacc ggcgcgggcg tttttgtccg gtaactgctg
      181 taggtgcggg agagaatgaa cggcagcggg ccgggcaggg caaggtcggt ttcgccggga
      241 aggaccttcg caccgagcag cggattgacc ggatgaccgg acgtcacccc gccggggcac
      301 accgaacagg ccacaccggt gggggcacca atacgtaccc cggcagaacc ctgaacaatg
      361 ctaccgccat actgcgtcat gtcgccctga cgcgccgccg gttttccgct catcgtaaaa
      421 tccttttcta ttattttaat gacagtatct tcatacgaaa aagcgaagaa aaacttgtgg
      481 tcacaatgaa ttgaagattt ttaggaatat atttagaacg ttacaaaaga gaaggttcgg
      541 atagggtatt tagagaggaa tcaggtgtgc tagtggccct ttgttgatcg ttcaccaaag
      601 gacccaataa taggcttaat cttccttcgg caagcactgc aaatgcccgt gccgcacgcc
      661 gcgctgggcg ataacgtcat cggcaaaatg ctgcacgtcg cccatatcgc ctttcagcac
      721 ggcgatttcc agacagtcgt cgtgattgat gtggacatgt agcgtcgcga cggagagatc
      781 gtggtgatgg tgctgggtgg agacaatacg gctggctaag tcgcgttttt cgtgttcata
      841 cacataggag agcaccgcga aaccttgcgt gccgtgctgc tgggtagctt cctgcgccag
      901 ggcgctacgc agaatatcgc ggatagcttc ggaacggttg ttatagccac gacgctggct
      961 caggctgtcc agcgtctcca gtaaatcgtc atcaagcgtg atggtaactc gttgcataga
     1021 cttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc gcgttttgta
     1081 gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga
     1141 tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg cgtaagtcgt
     1201 gggtgatgaa caggcaggcg gtgccaaact gttgttgcag ctttttcagc aggcgaatga
     1261 cgcccgcctg taacacgaga tcaaggttag aaacggcttc atccagaatc agtagtttcg
     1321 gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag ctgaccgccg cttaactgcg
     1381 gtgggcgttt gtctagaacg ctgtcatcga gatcgaccgc cttcagcatt tcgctggcac
     1441 gcgccagttg ttcggctttt ttcagcgata gcaggtggcg catcggttca cgcaggatct
     1501 cgcgcacggt ttttcgcgga ttcacggcgc tgatggagtc ctgaaatacc atctgaatat
     1561 cgcggcggaa cgctttacgc tgggcgcgat tgagctttgc caacggttcg ccgcgccagc
     1621 taatattccc ttgtgaggga gactctaagc ctaccagcag ccgcgcgaga gtgctttttc
     1681 cgcagccgct gcgccccagc agagcgacgg tttcgccgct tttcagggtc agggaaacgt
     1741 tattcagtac cgcctgatgc tgatgttttc cgctaaatcc accgtgcgca tagtgatgtg
     1801 aaaggtcgca gacgttaagt aaagtcatga tgccagctcc ataccgtaga gggcgagatg
     1861 agcggaaacc aggctgcgcg tcaccgtatg tttgggggcg ttaaacagcg tttctacatc
     1921 gccctgttcg acaattttac cgtgtgacat caccgccacg tcatccgcca gacgcgccac
     1981 aacgcccata tcatgggtga ccagcagcat tcccggtgct tgtttttgca taatgctttc
     2041 cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg tcggtggtcg gttcatcggc
     2101 gatgataaac ggcgattcac acaacaccgc catcgcaatc atcatgcgct gcaacatgcc
     2161 gccgctcatc tcgaacgggt acagcttcaa cacgcgcgcg gtgttttcca accccaccgc
     2221 ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cccagcgcca ggcaggtttc
     2281 acgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg ctgcgcgggt tctgcatgat
     2341 ggtggcaatt ttgatgccgc gcagggcaca gggagaaacc ggtttgccat cggctaaaat
     2401 ttcccccgcc gtctggcgaa cgcctgcggg cagaatgccc agcgtcgcgg cgcaggtcag
     2461 tgatttcccg ctgccgctac cgccgactaa cgccagcaca cgcccgcgtt taagggttaa
     2521 cgatacgccg tgcaccagcg gttgtgcggc ctgtagcgcg atattacgta gttcaatctg
     2581 ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat ccagatgatc gcgcagtgcg
     2641 tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt
     2701 tgcagcggct gggtccagat atactgacgc gcgtcgttaa tcatcacgcc ccattcggcg
     2761 gtcggcgcgg tcacgccgag accgaggaag gacatccccg cgacgtgtag catcatatga
     2821 ccgatatcca gcgtcgccag caccagcagc gaggggatca ccgcacctgc cagatgatca
     2881 ataaacaccc gcacatggcc cgcgccagaa agccgtgatg ccagcacaaa ctcgcgctgg
     2941 cgcagtgaaa tcaccaggct acgcaccatg cgtgcatacc acgcccagtg cgacagggcg
     3001 atggctataa tcacattggt caacccggtg ccgagcacgc cgaccataaa gaacgacaga
     3061 atcgaggttg ggaaggtcat aaacatatca gcgacgcgca tggtggcctg atcaacgcgc
     3121 ccgccaatca gcccggcgct gccgccaata atcagcccta atgtcagcac caacagcagg
     3181 caggccatta ccgagccgag cgacacgcgg gtcgccgcca tcagccgcga gaaaatatcg
     3241 cgccctaaat gatcggtacc cagccagtgc tgcgcatccg gtgaaagcag gcgcgacggc
     3301 agatcaatcg cctgggggtc atacggcagc caccactggc tggtgagcgc aatcagcgcc
     3361 agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag gaaaaagttc
     3421 acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg agtaaatcaa
     3481 caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg
     3541 gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga
     3601 tgttttcgat aatcatcgtt ccgccaatca gttcgccaat gtgcatcccc acggcggtga
     3661 tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg tcgttcaggc
     3721 cgcgtagccg agcccaggtg acgtgacgct gaccggcgac gtccagcata ctggcgcgca
     3781 gcagccgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc aaaatgatgt
     3841 gctgccagcc gccgtagccc atcgcgggta gccattgcag atataccgaa aacgccatca
     3901 ccagcaaaaa cgccagccag aagttgggca tcgacacgcc gagaaacgcg atgaaacgta
     3961 cggcgaaatc cggcagacga tcgcgatggc gcgccgccca gataccgagc ggtacggaag
     4021 tgagcagaat taacaccagc gcggcacctg caagttccag cgtggcgggc aggaagttca
     4081 gcatatcgtc cagcaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg
     4141 ccttccacaa ccaggtaccg tactggacgt acagcggctg atccagcccc agcatggtgc
     4201 gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaga taatcgagcg
     4261 ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg gcgagcacca
     4321 tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt
     4381 aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatgttac ccagctccgg
     4441 ttttgatacc accatcattg agatgtaact gataggcaga taaaccgcct cgtcatgcag
     4501 acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac
     4561 ttcgccgatc tctttatcaa tcagcggctt gtcggctaaa ccttgctgtg cctggaagtc
     4621 agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca
     4681 ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc
     4741 ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc
     4801 cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg
     4861 accatttttc tcgcggatgt ctttgcctgc aggcagcgtc caaccggctt tttccagcaa
     4921 cgctttcgct ttctgcggat cgtactggcg cggtttcagg ccgaggttgg cgtagggcac
     4981 agaaggggca aacagggtgt cggcgacctg ctgggtacca tacaacgcgt tatcaatcag
     5041 cgattttttg ttcaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc
     5101 tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gagtgtggta
     5161 agccgggttc tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta
     5221 cagcaggtcg atatcgccag tttcaaacgc caccgcacgg gtagtcgggt ccgggatcac
     5281 gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt tacggacgaa
     5341 gacatcgtac tgattcagtt tcgattcctg taaaacccac ggcccggtgc caatcggcgc
     5401 tttaattccg ttcatggttt cgtggttttt aaactgcgag ggtgcgataa aacggaaagg
     5461 acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg tgatttgcag
     5521 ctcgttttta ctgagtgctt tgacatcaac aatctggttt gccagctcca gccaggcgtg
     5581 acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctctg catcaaacgg
     5641 ttcgccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt taccatcttc
     5701 tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac
     5761 caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt
     5821 tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac
     5881 gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg agagcatggt
     5941 aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat
     6001 actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa
     6061 tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg taggggtgca gatcgccagg
     6121 cttaaattgt ctagctgaca atggctgact gacagtgatg agtgataggt gctgtctacg
     6181 ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag
     6241 atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatctc ggcatgttcc
     6301 ccaaggctga atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg
     6361 atatcgcagc caacttcgcc ttcatcgctc aacagcaggg cgatatcgtc accgctatgg
     6421 cttaagttga accatagcgg cgtttccggc gcgaatgccg gtttgccttg ttcgccatag
     6481 atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt
     6541 tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaagccg gtggcagtgg agctgcgctt
     6601 aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa
     6661 tgaaagtgcg tatcgtaaca cttgtcgcct catcccggta accgactttt cggtctgcct
     6721 ggccccagta aaatcgccag tttgctaccg cctttggtgg tttccatcca gattttacac
     6781 acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccaaatt
     6841 aacaatgaaa gaaataccac catggtggac atccccagac gatggcccat catccgtggt
     6901 tctaaaatat tgccgatgac catatggacc actaaaaaca atgcgccgac cagaatacat
     6961 tcgtaaatac cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgca
     7021 ccgatattgg gcacgtagtt gagcaaaaac gccagtaccg cccacatcag cgcaaactgt
     7081 acgcccataa gttccagccc cagccagacg ataacgcctg tccataaact tagcagtgtt
     7141 ttcaatgcca gataatgcga aacgccttta agtgcgcggt gtagtccggc gatgtgaatt
     7201 tgtggattat tcaacgcgaa acgcattttg taagggacgt ggcgcacttc aaacagcata
     7261 aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt
     7321 agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat tcgctccggt
     7381 gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc
     7441 tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg
     7501 accagcgcag ttagtgcgat cagcatcacc accaccacaa tcgtaatggc aacggggcgt
     7561 tgtactcccc gacgaataaa ccaggtgacg agcgggttga ggacgatggc aaaaaacagc
     7621 gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc
     7681 agcgaggcca gcttgagcag aatgtgcatg cccgttttat cgggttgagg ggtttccatt
     7741 ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga
     7801 ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg aaacactgtt gtaaaaatgt
     7861 ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt
     7921 gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct
     7981 cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgcttttt gggcaggatt
     8041 ggttatcagc ctgcaatatt tcgccacctt gctgagccgc cctcatgccg gacgttacgc
     8101 cgatttgctg ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttttgagcgg
     8161 tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact
     8221 gtgcctgggg cgcgtcatcc ttgggattgg gcaaagtttt gccggaacgg gatcgaccct
     8281 gtggggtgtt ggcgtggttg gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat
     8341 tgtcacttac ggggcgatgg cgatgggtgc gccgttaggt gtcgtgtttt atcactgggg
     8401 cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca ttttgttggc
     8461 gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct
     8521 tgggcgcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgttat
     8581 cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct
     8641 gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg
     8701 tatcggcggc ttaaacgtag cgatgatttg ctttagcgtt gagataatcg gcctgctact
     8761 ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggcagg
     8821 gttttcgctg gtgttcccgg cattgggcgt agtggcggta aaagcggttc cacagcaaaa
     8881 tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgaccgg
     8941 accactggct gggctggtga tgagttgggc gggcgtcccg gtgatttatc tggcggcggc
     9001 gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ctccggaaca
     9061 cgtccctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt
     9121 tttctgcggt ggtctgcgct ttttgctgat tgtcagctgg cagcgtaatt tgcatggtca
     9181 gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg
     9241 agataatgct gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta
     9301 ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccaacaccg
     9361 ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttttgc ccagtggcgt
     9421 cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat
     9481 ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtagcgt
     9541 tactttccgc gaccgacccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa
     9601 gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc
     9661 tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc
     9721 tttaactcgc ctgcaaagcg ttgatttcag atttatctgc cagtcttttc aacagcatat
     9781 tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt
     9841 aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga
     9901 aggcaatgaa gaagaaggcc agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg
     9961 gtgccagcca ccagtgacga ctctgacgca ggcggttaaa aacgtgcacg tcgaggatct
    10021 gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt
    10081 gggcgagcgc gccgaatccc tgccaggaac ccatatagaa cagcgacgag atgacgtagg
    10141 agatcagtaa cgccgggatc atcaccgcaa agatgatacg acgggccagc ggtgcgccaa
    10201 aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg aaagctaaat gcgccccagg
    10261 tggtatggaa accaaaaatg gagacgggaa gctgaaccag atagttactg gaggtgatca
    10321 ccagcagatg aaataacgat aaccagaaca acgccttata gcgttgagtt tgcgagaaaa
    10381 cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata cgtacgacac
    10441 gctattttac cttgattaaa taacgttctt accctttttt cgagccgccg catgatactg
    10501 ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg
    10561 actcacaggg cgtaaactag cgccgttttt ttatgtgatg agaagaaaat gaccgatctc
    10621 ttttccagcc ctgaccacac actcgacgca cttggcctgc gctgcccgga accagtaatg
    10681 atggtgcgca aaacagtgcg caatatgcag ccaggcgaaa cgttgctgat tatcgccgac
    10741 gatccggcca ctacccgcga tattcctggg ttttgtacct ttatggaaca cgaactggtt
    10801 gctaaagaga cggatgaact gccttatcgt tatttgattc gcaaaggcgg ttgatgggga
    10861 aagattggct tcgatgccgc cttttcccct caccctaacc ctctccccag aggagcgagg
    10921 ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac
    10981 gcattcgctg tcaccagcac cgtcgcaccc gtatctgcca gcaccgccag ccatagcccg
    11041 gtcattccta acagtgtggt gacgaggaag atccctttca gccccagcgc aatagtgatg
    11101 ttctggcgga tattggcgtg agtggcgcgt gccagttcaa tcatttgcac caggccgcgc
    11161 aggtggttat gggttaatgc tgcatcggcg gtttccagcg ccacgtctgt gccactgccc
    11221 attgcaatcc cgatggcggc ggctttcatc gctggcgcgt cgttaatacc gtcaccgacc
    11281 atcgccagcg gcgcatgttg attcagctcg gtcaccgctt tgactttatc ttccggcaac
    11341 aggcccgctt taaactctag ccccagttcc ccggcgattg ccgccgctgc gcgtggatta
    11401 tcgccggtga ggatcacccc tttgacgccc agcgcgttca gttcactgat ggcagttgca
    11461 gcatcggcgc gcagggtatc ctgtaacgca atgacaccaa gcacgtcatc gttacgtact
    11521 accagcacta ccgtttgccc ggcgctttcc agttcgttaa ttaaaccagc aaatgcatca
    11581 gcgggatgtt tcccggcagc gcatatcaac acgcgctcac cgttaacctg cgcttcaatg
    11641 ccagacccaa ccagcgcccg ctgtgattgg gcggcgggaa tggcgagttc agcaacctgt
    11701 gcttctcgca cgatggcttg cgccagtgga tgcgtcgcgc cttgctcgac cgccgctgcc
    11761 agtgtcagca gttcggattc actaataccc gttgccggat gaatcgcggt aacgcgcggt
    11821 ttaccgacgg tcagcgtacc ggttttatca aacgccacct gggtaacacg acccagttgt
    11881 tccagcgccg ctccgccttt aattaacgcc ccacgacgcg ctgctgctgc cagcccggag
    11941 gtaatcgccg caggcgttga gataactaac gcacacgggc agccaatcag cagcagcgtc
    12001 agccctttat aaatccactc ttgccagctg gcggcaaaca gcagtggtgg caccagcgtt
    12061 accagcagag cgacggccat aatcgctggc gtgtaaatac ggctgaaacg gtcgataaac
    12121 cgctcaatgg gagcgcgacg ctcttcggct tcttcgatca gtttcagaat ccggtcaatg
    12181 gcgctggcac ccggttctga cagcacttcc agcattacca gacggtctac gctggtggcg
    12241 cctgcaggaa ctttatcgcc cgtcgcgcgc tccacaggaa tggattctcc ggtcagggcg
    12301 ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgcccacct
    12361 gccgcaactt caatcacatc gccaggccgc aggctgttta ttgccacctc ttcacgctca
    12421 ccgttacgca gacgcgtggc ggtttctggc ttcagcgcca ttaacgcgct gaccccttga
    12481 cgcgcgcgac tggcggccca gccctccagt cgttcgccaa tcaaaaacag cagcaacacc
    12541 atcgcagctt cagccgttgc gccaataaac agcgcaccaa tagcggctac gctcattaac
    12601 gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg agcaatcggg
    12661 tacagcccaa ccagcgtggt cgcgataaac gccagttgcc cgaacggatg attgaactgc
    12721 tccagacccc agctgattgc catcatcacg attagcgtaa tcagcggcag attctctttc
    12781 aggcgtgatg cttgtggttc gtcggcggac tgttcatcgc gcagggaata gcctgctttt
    12841 tgcaccgcag attcaacctg ggcacggatg tcattgtcgg catcgaccac cagtttttcg
    12901 gtggcgaaca acacctgcac ctgattcacg cctgcaagct ggcgcacggc attttctacc
    12961 ttgcgcgcac aggcggcgca gtccatgccg ctgactttcc agctatagcg ggtgccggag
    13021 acgttttcag agagagttgg cgtgctggaa catgcgccgt cgcagcaaca gtcgttggcg
    13081 ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg
    13141 tcaggagtcg acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc
    13201 tggagtcgac tccagagtca agttttatca gagatacagc gagcggacga tgaggaaatg
    13261 accggcaaag tagcaggccg cagcaatcgc gttatctgcc cggaagcggc ggcgatagtg
    13321 gctacccagc cagacaaagt tactgataaa cagcaacgaa gcgccgacaa acgcagagag
    13381 cgccggagct gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt
    13441 catgccgata aacgtgcaga taggccaacg atactcttcc agacgcgtcc agataatcgc
    13501 cagtaacagc gcacccagaa ccagcaacac cagtggcaga ggccagaaga aagagagcgt
    13561 catctgactg gcaaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc
    13621 gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcgcacag
    13681 ccctgccagc accagatagc taatggcgtc gaacatcggc gcttgccagg ccagtaacag
    13741 caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga
    13801 tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt
    13861 tccttgaatt cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg
    13921 gcgagggtgg acggatgaca acaccataac tggcagggta tgcttatttc cgcattttcc
    13981 gatgagggat aaagatgagc aaaccacctc ttttctttat tgttatcatt ggcttaattg
    14041 tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat aatgatatgg
    14101 ctccgctcca gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc
    14161 gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctac gaggcaagct
    14221 tcaaaccgca aagcggagga atggagcaga cgtttcgcct cgacgctcag cagtaccatg
    14281 ccctgacagt gggcgatata ggtacgctga gctataaagg aacgcgcttt gttagctttg
    14341 taggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa
    14401 acacgccgaa aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttag
    14461 gtaaagtgga tttcaatagc gccagctgca taccatgcat cagcacggta aaaatcagcg
    14521 ccacgttaac gaaaatattc agcgggcgcg ggaagggatg caccagattg aggattaaaa
    14581 atccccaaac gcagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt
    14641 gcttcgcgtt gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaatgac
    14701 cagtttgccg gaacagtggg cagaccgttt tcgacttcgc tttcgacata aatcagggct
    14761 tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa caagccacgg
    14821 cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac
    14881 attgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt
    14941 aactgctgag aaaccgcgcg atccatctca atcaacgtgg cccccgcagc atagcgcgac
    15001 aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca
    15061 atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga
    15121 cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt
    15181 tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca
    15241 catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta
    15301 gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca
    15361 aatggcgaaa gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc aaaaagagca
    15421 gaccccggaa aaagagacag aagttcagaa tgaacaaccg gttgtagaag aaatcgttca
    15481 ggcgcaagag cctgcgaagg cttcggaaca agccgttgaa gagcagccgc aggcgcatac
    15541 tgaggccgaa gcggaaactt ttgctgccga cgttgtggaa gtcactgaac aggttgttga
    15601 aagtgaaaaa gcgcaacctg aagcggaagt tgttgcacag ccggaactgg tcgtagaaga
    15661 aacgccggag ccagtggcta tcgagcgtga agagctgccg ttgccggaag acgtcaacgc
    15721 cgaagaggtt tcgccagaag agtggcaggc cgaagcggaa accgtagaga ttgtcgaagc
    15781 ggcggaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag cacaggcgct
    15841 ggctgccgaa gcagcagaag aggcggtgat ggtggttcct ccggcagaag aagagcagcc
    15901 ggtagaagaa atcgctcagg agcaggaaaa accgaccaaa gaaggtttct tcgcgcgcct
    15961 gaaacgcagc ctgttaaaaa ctaaagaaaa tctcggttcc ggatttatca gcctgttccg
    16021 cggtaaaaaa atcgacgatg atctgtttga agagctggaa gaacagctgt tgatcgccga
    16081 tgtgggcgtg gaaaccacac gtaaaataat caccaatctg acggaaggcg catcccgcaa
    16141 gcagcttcgt gatgccgagg cgctttatgg cctgctgaaa gaagagatgg gcgagattct
    16201 ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt
    16261 gggcgtcaac ggtgtgggta aaaccacgac gattggtaag ctggcacgtc agtttgagca
    16321 gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcggcag cggttgaaca
    16381 gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata ccggggcgga
    16441 ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata tcgacgtcct
    16501 gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag agttgaagaa
    16561 aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat
    16621 tgatgccagc accgggcaga acgcggtaag ccaggccaaa ctgttccatg aagccgttgg
    16681 cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcggag taattttctc
    16741 ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga
    16801 tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca
    16861 atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc
    16921 gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg
    16981 aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg
    17041 tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag
    17101 attggtatga ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg
    17161 gcgatcccgc tgattatcgc cggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg
    17221 gcactggata aagtcgggct actggacaaa gcgaagaact ttcctattca gctttccggc
    17281 ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc ggtactgctg
    17341 gcggacgaac cgactggtaa cctggacgac gcgttgtcgg aaggcatttt acgtctgttt
    17401 gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat caacctgatt
    17461 tcgcggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc
    17521 catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggcgggcgtc tggatcgctt
    17581 ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc
    17641 ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt
    17701 ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt
    17761 tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca
    17821 ggcggcgacg caatattatc cgtcaccgca aatcactgtt tatctgcaaa aaacgctgga
    17881 cgatgacgcc gctgcgggtg tggtggcaca gttacaggcc gagcaaggcg tagagaaagt
    17941 gaattatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg
    18001 tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaaact
    18061 cgattttcag gggacggaat cactgaatac gctgcgtgat cgtatcaccc agatcaacgg
    18121 cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt taaccgggct
    18181 ggtcgggcgc gtttccgcga tgatcggcgt gttgatggtg gcagcagtgt tcctcgtcat
    18241 cggcaacagt gtgcgtctga gcatctttgc tcgccgtgac tccattaacg tgcagaaact
    18301 gattggtgcg acggatggtt tcatcctgcg cccgttcctg tacggaggcg cattgctggg
    18361 attttctggc gcattgctgt cattaatttt gtcagaaatt ctggtactgc gtttgtcatc
    18421 ggcggttgcg gaagtggcac aggttttcgg aacaaagttt gatatcaatg gcttatcgtt
    18481 cgatgaatgc ctgctgttgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct
    18541 tgccacggta caacatttac gccactttac gccggaataa taaaagcgtg ttatactctt
    18601 tccctgcaat gggttccgta gcagggaaag agaccccgtt gtctcttccc ggtatttcat
    18661 ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt gtggataaaa
    18721 tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctctg gcacagttgt
    18781 tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc
    18841 aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt
    18901 ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg
    18961 atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc
    19021 gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc
    19081 tgatgaaagc agtgcgccgt tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg
    19141 tccactggat caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag
    19201 ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc
    19261 tgggctggtt taatcaggat gaagttgaaa tggtggcccg tgaactgggc gtaaccagca
    19321 aagacgtacg tgagatggag tcgcgtatgg cggcacagga catgaccttt gacctgtctt
    19381 ccgatgacga ttccgacagc cagccgatgg ctccggtgct ctatctgcag gataaatcat
    19441 ctaactttgc cgacggcatt gaagatgata attgggaaga gcaagcggca aaccgtctga
    19501 ccgacgcgat gcaaggtctg gacgagcgta gccaggatat catccgcgcg cgctggctgg
    19561 atgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgagc
    19621 gtgtgcgcca gctggaaaag aatgcgatga aaaaacttcg cgctgccatt gaagcgtaat
    19681 ttccgctact cagcagagaa ccctggatga gaatccgggg tttttgtttt ttgggcctct
    19741 gcaataatca gtttccccct ccggcaaaac gccaatcccc acgcagattg ttaataaact
    19801 gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag tatgctgcta
    19861 aagcacgggt agtcatgcat aaaacgaaat aaagtgctga acaacaacat cacaacacac
    19921 gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc gttactggca
    19981 ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa agtcgccgtc
    20041 gtaggcgcaa tgtccggtcc ggtggcgcag tacggtgacc aggagtttac tggcgcagag
    20101 caggcggttg cggatatcaa tgctaaaggt ggtatcaaag gcaacaaact gcaaatcgta
    20161 aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac
    20221 gacggcatta aatacgtgat tggtcacctc tgctcttcat caacgcagcc agcgtctgac
    20281 atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcacc agagctgacc
    20341 gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca ggggccgacg
    20401 gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa
    20461 cagcaatacg gcgaaggtct ggcgcgagcg gtgcaggacg gcctgaagaa aggcaatgct
    20521 aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctcaac gctggtggcg
    20581 cgtctgaaga aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg
    20641 caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg gccggaaggc
    20701 gtggctaacg tctcgctgtc taacattgcg ggtgaatcag cggaaggact gctggtgacc
    20761 aagccgaaga actacgatca ggttccggcg aacaaaccca ttgttgacgc gatcaaagcg
    20821 aagaaacagg acccaagcgg cgcgttcgtc tggaccacct acgccgcgct gcaatctttg
    20881 caggtgggcc tgaatcagtc tgacgatccg gctgaaatag ccaaatacct gaaagcgaac
    20941 tccgtggata cggtgatggg gccgctgacc tgggatgaga aaggtgatct gaaaggcttt
    21001 gagttcggcg tatttgactg gcacgccaac ggcacggcga ctgatgcgaa gtaatcatta
    21061 atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt catgccggat
    21121 gcggcgtaaa cgccttatcc ggcctacaag attcaaagaa atcagtaaat tgcagcacac
    21181 attgtaggcc tgataagcgt agcgcatcag gcaatatcac ttgtgaaatc aaacttgacg
    21241 attaacgctt ctcccagccg ccctgttgtg ccgtaaatcc cagtgcctgc ataaacgctg
    21301 tcatcacgcc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acgccaggat
    21361 tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg gtgacttccc
    21421 tcacacgcag ggaatccagt gccccctcgg tgccgcttaa ggttacccgc acggccgcga
    21481 gaaggcgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg
    21541 aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt
    21601 ctaatcgaat gatggtcagc ttcatgggga acccgtgtaa atcacaaaag tggaaccagt
    21661 gtagcgaaat aatttaatcg gaggctttct cttttttaat tcttttggca ggtgattaat
    21721 tttttaacag caataattac aaagttaaaa cattagagaa tgaaaaatac ccagcataat
    21781 cccct
//
LOCUS       DAZGTJ010000085        21202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000085 DAZGTJ010000000
VERSION     DAZGTJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.085"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(372..1568)
                     /gene="hemY"
                     /locus_tag="ACHK8E_003861"
     CDS             complement(372..1568)
                     /gene="hemY"
                     /locus_tag="ACHK8E_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP0346070.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1571..2782)
                     /gene="hemX"
                     /locus_tag="ACHK8E_003862"
     CDS             complement(1571..2782)
                     /gene="hemX"
                     /locus_tag="ACHK8E_003862"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP0346071.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     ADAPAAAPQGE"
     gene            complement(2804..3544)
                     /gene="hemD"
                     /locus_tag="ACHK8E_003863"
     CDS             complement(2804..3544)
                     /gene="hemD"
                     /locus_tag="ACHK8E_003863"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP0346072.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3541..4482)
                     /gene="hemC"
                     /locus_tag="ACHK8E_003864"
     CDS             complement(3541..4482)
                     /gene="hemC"
                     /locus_tag="ACHK8E_003864"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP0346073.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4869..7415
                     /gene="cyaA"
                     /locus_tag="ACHK8E_003865"
     CDS             4869..7415
                     /gene="cyaA"
                     /locus_tag="ACHK8E_003865"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP0346074.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7455..7775)
                     /gene="cyaY"
                     /locus_tag="ACHK8E_003866"
     CDS             complement(7455..7775)
                     /gene="cyaY"
                     /locus_tag="ACHK8E_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP0346075.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7823..9880)
                     /locus_tag="ACHK8E_003867"
     CDS             complement(7823..9880)
                     /locus_tag="ACHK8E_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346076.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10222..10776)
                     /locus_tag="ACHK8E_003868"
     CDS             complement(10222..10776)
                     /locus_tag="ACHK8E_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP0346077.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11118..11321
                     /gene="yifL"
                     /locus_tag="ACHK8E_003869"
     CDS             11118..11321
                     /gene="yifL"
                     /locus_tag="ACHK8E_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0346078.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11358..12182
                     /gene="dapF"
                     /locus_tag="ACHK8E_003870"
     CDS             11358..12182
                     /gene="dapF"
                     /locus_tag="ACHK8E_003870"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP0346079.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12179..12886
                     /gene="yigA"
                     /locus_tag="ACHK8E_003871"
     CDS             12179..12886
                     /gene="yigA"
                     /locus_tag="ACHK8E_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP0346080.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12883..13779
                     /gene="xerC"
                     /locus_tag="ACHK8E_003872"
     CDS             12883..13779
                     /gene="xerC"
                     /locus_tag="ACHK8E_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP0346081.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13779..14495
                     /gene="yigB"
                     /locus_tag="ACHK8E_003873"
     CDS             13779..14495
                     /gene="yigB"
                     /locus_tag="ACHK8E_003873"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP0346082.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14579..16741
                     /gene="uvrD"
                     /locus_tag="ACHK8E_003874"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14579..16741
                     /gene="uvrD"
                     /locus_tag="ACHK8E_003874"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP0346083.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16786..17688)
                     /locus_tag="ACHK8E_003875"
     CDS             complement(16786..17688)
                     /locus_tag="ACHK8E_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFP0346084.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17771..18535)
                     /gene="yigE"
                     /locus_tag="ACHK8E_003876"
     CDS             complement(17771..18535)
                     /gene="yigE"
                     /locus_tag="ACHK8E_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP0346085.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18697..18753
                     /gene="ysgD"
                     /locus_tag="ACHK8E_003877"
     CDS             18697..18753
                     /gene="ysgD"
                     /locus_tag="ACHK8E_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP0346086.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18905..19855
                     /gene="corA"
                     /locus_tag="ACHK8E_003878"
     CDS             18905..19855
                     /gene="corA"
                     /locus_tag="ACHK8E_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP0346087.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19891..20388)
                     /locus_tag="ACHK8E_003879"
     CDS             complement(19891..20388)
                     /locus_tag="ACHK8E_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP0346088.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEK
                     DNFQQ"
     gene            complement(20385..>21202)
                     /locus_tag="ACHK8E_003880"
     CDS             complement(20385..>21202)
                     /locus_tag="ACHK8E_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP0346089.1"
                     /translation="PCTLFETITLFDDFSADDMQYGDMEEQDFLSLGLSDISAKVDPY
                     RLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFSFFGQYKTLIQD
                     LIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDISSTPTIGYRPLL
                     LQKIKTELLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVA
                     QDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         5045 a         5395 c         5437 g         5325 t
ORIGIN      
        1 gcaggagaaa tattcactca ggaagttatt actcaggaag caaagaggat tacagaatta
       61 tctcataaca agtgttaagg gatgttattt cccggttctc tgtggcataa taaacgagta
      121 gatgctcatt ccatctctta tgttcgcctt agtgcctcat aaactccgga atgacgcaga
      181 gccgtttacg gtgcttatcg tccactgaca gatgtcgctt ctgcctcatc agacaccatg
      241 gacacaacgt tgagtgaagc acccacttgt tgtcatacag acctgtttta acgcctgctc
      301 cgtaataaga gcaggcgttt ttttatgtat cagaaaggtc ccggaggagc ttgcctccgg
      361 gtgagaaaga gctactgtgg cgggttgttc tgcaacgtta acatcaaacc gtcgcgacgc
      421 atcgctgcgg cttcttccgg cttgtgcagt ctgtccagcg cgtcggcaag ccatgcgtaa
      481 tcgtaggcgt ccggacgttg tttcagcgct gcgcggaagg cgagcgatgc ttcctgccat
      541 tcgccgtgct tcatcagcga ctgacccagt gtgctccaca acagcgggcg atcgcccacg
      601 tttttgatct gttggcgcag cactttttca agctgttccg ggttattggt tttcagacgc
      661 gggatcggca gcagcaggcg atcgtcatac tggcgtttca ggccgtcgat gataatttgc
      721 tgggcagtat catgatcgtc acattcaata agatgttccg ccattgccac ctgtaacgcc
      781 acctgatgac gcgttttccg gctttggttt ttccaccagt tacgcaaacc ttcactaccg
      841 ttatcggcac gcgcctgatc catcaggcca atccatgcct gttgttccag cattgcacga
      901 tgttcttcat caccaacatg ggctttcgcc attgatggga tgatatccag cagcgaactc
      961 catgcaccag tgcggatata cgcctgttcc gccagacgta atacttccgg atggcgtggc
     1021 gtaacttcca gcagcttatc aacgccgtga cgtgctgcat ggttttcatt acgggccagt
     1081 tgcagacgta cacgggtgat ttctaccgga atggtgtcat tgccggccag ctccgctgcg
     1141 cgttccagat gttggttggc gcgtgcttca tcaccacgtt gttgcgcggc ttcggctgcc
     1201 agtagatagt tcaccaccgg ttgttcagcg tgatcggcat ttttcgccat cagcttttca
     1261 acttgctgat aatcgccttc cgccagtttc agcagcgcct gttcggtctg cttacgtgca
     1321 cggcgacgct tacgtccgac aaaccaccca cgggtgtgcg cgcctgtgcg gaagattcgc
     1381 cgcagtagcc actcaatggc aaacagcact accatcgcca gaatcaaaat aatcgcgagg
     1441 cccgtgacgc tagtttcaat attgtagttg tcggtctgga tcagcacgta accctgatgg
     1501 ccggcaatca tcgggccaac cacgatcccc gcaatcagca acacaaagag caataacact
     1561 tttagcatgg ttattctcct tgcggcgcgg ctgccggagc atcagcttgc ggtgcaggtg
     1621 caggtgcagg tgcaggtgca ggtgcaggtg caggtttagc ttccgttgtc cccgctgccg
     1681 gttgtgccag caggttacgc acgcgagttt gcatcagttt ttccagcatc gcctggcttt
     1741 gcagggtttc cggaagatcc atcgagatat tttgctggct taactggtcc acctcgtcga
     1801 ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc acgtacccag gtggagacgt
     1861 tctccagcgc ctggcgataa gtctcttcct ggtgacgcgg tacagcttgt gctgcgacca
     1921 gcaggcgaga gcgaatattt tcgcgcagat agatatcctg atttggcgct aacagcggta
     1981 cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc cataaagttc tgccagcttt
     2041 tttgcagatt gatacgccat tcgctgatgg aactggaaag ctcttcaccg tcggaatcca
     2101 tcggcgaacc atcgctgtca ttatcagcca gacgcagatt atctacctga tttgaaagct
     2161 gattaagctt aaggatgata ccgtcataat ccacctgcga taccgcagaa aggctggcga
     2221 tatcatcggt aattgcccga cgaacggtaa tcagactcgg gtcattcata tccgccaggc
     2281 tggcatctgc acttttcagc aacgctgcag cggtcgtgac gtcttgatcg ctccacagct
     2341 tccgtcctgc gagtttcacc aggaaatcag cctgagccag cagccaggtt ttagcatcgc
     2401 tgccggaaat agtcgcgacc ttttgttgga cttcatccag ctgttttgcc agcgtttctt
     2461 gctgacgatt cgcctgctca agctgtacag cttgttgctt gataatgcct tccagctcag
     2521 ctttttggct ctcctgggct ttttgcagtg ccgtaagttg gttagccagg gcatcgctgg
     2581 tggcagtctg attgacggcc tgttgtttac cccagccata caaaccgacg cccgccgcca
     2641 gagcaatagc gatagccacc gcgctgagaa tcaatgcggt attgttctta ctcttttttt
     2701 ctgttgcgac aggttgtgac gtggtgtcca cggcctccct ggtctcttca accacggcgg
     2761 aggttttttc ttgttccgtc attatggctt cctgttatga gagttattgt aatgcccgta
     2821 aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc ttgccagccc agttcccggg
     2881 cgagtttcgc caaacgctca ctgacgacca atagtcgaca gcgtagtaac cagtgctcac
     2941 gataccattg tgggatcagc gaccagagtt gctgcaacat ttcaccgctg gtaacaacga
     3001 ccgtcgtcac ctcgcgggat tgccagcgca tcgcttcttc tgcaccatcg taatggatta
     3061 cgcatcgttg ataacattca caaaaagtga cctcagcacc gcgcgccgtc agggtatccc
     3121 caattagctc acgaccgcca ttgccacgta atatcagcgc acgtttgccc gcaatatttt
     3181 gtaattcagg taattgtagc aagacttcgc tgatttcccg atcctgcggg tagagaatct
     3241 tctgtccgct tacggtatgt agtgccagtg cggtggtgcg tccaatggcg aaataagtag
     3301 gtagtcgggg ccatttacga tcttgctgat gcagctgtga ttgggcaaaa gcaaccgcgt
     3361 gttgcgagag ggcaaacaac agatcgctct cccccagcgc cgccagttga tcagcaagtt
     3421 gtggtaattg tcgacccgga gaaaactcaa tcagtggaaa atgccaggcc acctgcccca
     3481 gtgtgcgcag acggctcact aactcttctc cagcgggaga cgggcgggtg acaaggatac
     3541 tcatgccggg gcgtctccat tatagacttc agcgaggatc tcgcgcgcgc cgttattcag
     3601 tagctcttct gccagcgaaa tccccatttg ttcggcatct tgcggcgcac cgcggcgttc
     3661 accgcgaata atctgcgaac cgtccggcgc gccgaccagc gcacgcagcc agatttcacc
     3721 atcaataagc tcggcgtagc taccaattgg cacctgacat ccgccttcga gacgggtatt
     3781 catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg tgattcagcg cggcaagcag
     3841 ctcgcgagtg cgtgtatcat caaggcggca ttcaataccg accgcacctt gtcctaccgc
     3901 cggaagagaa atctcgggtg gtaacgcggc gcgaatacgt gactccagac ctaaacgttt
     3961 tagtccggct acggcaagaa tgatggcatc gtattcgccg ttatccagtt tactcaggcg
     4021 agtgccgacg ttgccgcgca gggagcggat aatcagatcc gggcggcgtt cagccagttg
     4081 gcactggcga cgtaaactgg acgtcccgac gatactgcct gccggtaacg catccagact
     4141 ttcatagtta ttggacacaa aggcatcgcg aggatcttca cgctcgcaaa tggtgaccag
     4201 ccccagacct tgcgggaatt caaccggcac atctttcatt gaatgtacgg cgatatcggc
     4261 gcgattctcc aggagcgcga cttccagctc tttaacaaat aagccttttc cgcctacttt
     4321 cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc atcggtacca gttcaacgac
     4381 caggcccgga tggctcgcca tcaacttgtc tttgacatag tgtgcctgcc agagtgcaag
     4441 tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac atgcttgtta ccgtcattat
     4501 catccgtggt ccatcctaac atccttgcca gagcgatgtc agtgttgtgg tgaaacgtag
     4561 acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc ctgacaggcg tttcacgccg
     4621 tcgtaataag gaatttacag agaataaacg gtgctacact tgtatgtagc gcatctttct
     4681 ttacggtcaa tcagcaaggt gttaaattga tcacgtttta gaccattttt tcgtcgtgaa
     4741 actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt tgacatacga aatatcccga
     4801 atgccgcgtg ttaccgttga tgttggcgga atcacagtca tgacgggtag caaatcaggc
     4861 gatacgtctt gtacctctat attgagactc tgaaacagag actggatgcc ataaatcaat
     4921 tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt ccaacaggtc tacagtctac
     4981 tgccgacatt gttgcactat caccatccgc taatgccggg ttaccttgat ggtaacgttc
     5041 ccaaaggcat ttgcctttac acgcctgatg aaactcaacg ccactacctg aacgagcttg
     5101 aactgtatcg tggaatgtca gtacaggatc cgccgaaagg tgagcttcca attactggtg
     5161 tatacaccat gggcagcacc tcgtccgtag ggcaaagttg ttcctctgac ctggatatct
     5221 gggtctgtca tcaatcctgg ctcgatagcg aagagcgcca gttactacaa cgtaaatgca
     5281 gcctgctgga aagctgggcc gcctcgctgg gcgtggaagt tagcttcttc ctgattgatg
     5341 aaaatcgctt ccgtcataat gaaagcggca gcctgggggg cgaagattgt ggctccaccc
     5401 agcatatact gctgcttgac gaattttacc gtaccgccgt gcgtctcgct ggtaagcgta
     5461 ttctgtggaa tatggtgccg tgcgacgaag aagagcatta cgacgactat gtgatgacgc
     5521 tttacgcgca gggcgtgctg acgccaaatg aatggctgga tctcggtggc ttaagctcgc
     5581 tttctgctga agagtacttt ggtgccagcc tttggcagct ctacaagagt atcgattccc
     5641 catacaaagc ggtgctgaaa acactgctgc tggaagccta ttcctgggaa tacccgaacc
     5701 cacgtctgct ggcgaaagat atcaaacagc gtttgcacga cggcgagatt gtgtcgtttg
     5761 gcctcgatcc ttactgcatg atgctggagc gtgttactga atacctgacg gcgattgaag
     5821 atttcactcg tctggattta gtacgccgct gcttctattt aaaagtgtgc gaaaaactca
     5881 gccgtgaacg cgcctgcgta ggctggcgtc gtgaagtgct aacccagtta gtgaaagagt
     5941 gggaatggga cgatgctcgt ctggcaatgc tcgataaccg cgctaactgg aagattgatc
     6001 aggtacgtga ggcgcacaac gagttgctcg acgcgatgat gcagagctac cgtaatctga
     6061 ttcgctttgc gcgccgcaat aaccttagcg tctccgccag cccacaggat attggcgtac
     6121 tgacgcgtaa actgtatgcc gcgtttgaag cgttaccggg taaagtgacg ctggtaaacc
     6181 cgcagatttc gcccgatctc tcggaaccga atctgacctt tatttatgtg ccgccgggcc
     6241 gggctaaccg ttcaggttgg tatctgtata accgcgcgcc aaatattgag tcgatcatca
     6301 gccatcagcc gctggaatat aaccgttacc tgaataaact ggtggcgtgg gcatggttta
     6361 acggcctgct gacctcgcgt acccgcttgt atattaaagg caacggcatt gtcgatttgc
     6421 ctaagttgca ggagatggtc gccgatgtgt cacaccattt cccgctgcgc ctgcctgcac
     6481 cgacgccaaa agcgctctac agcccgtgtg aaattcgcca tctggcgatt atcgttaacc
     6541 tggaatatga cccgacagct gcgttctgca atcaggtggt gcattttgat ttccgtaagc
     6601 tggacgtctt cagctttggc gagaatcaaa attgcctggt aggtagcgtg gacctgctgt
     6661 accgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa cggcgagcag tcgatgatcg
     6721 aagccctgaa aaccattctc ggtaaaatgc atcaggacgc cgcaccgcca gatagcgtgg
     6781 aagtcttctg ctacagccag catctgcgcg gattaatccg tactcgcgtg cagcaactgg
     6841 tttctgagtg cattgaactg cgtctttcca gcacccgcca ggaaaccggg cgcttcaagg
     6901 cgctgcgcgt ttctggtcaa acctggggct tattcttcga acgcctgaat gtatcggtac
     6961 agaaactaga aaatgccatc gagttttatg gcgctatttc gcataacaaa ctgcacggcc
     7021 tgtcagtgca ggttgaaacc aatcacgtca aattaccggc ggtggtggac ggctttgcca
     7081 gcgaagggat catccagttc ttcttcgaag aaacgcaaga cgagaatggc tttaatatct
     7141 acattctcga cgaaagcaac cgggttgagg tgtatcacca ctgcgaaggc agcaaagagg
     7201 agctggtgcg tgacgtcagt cgcttctact cgtcatcgca cgaccgtttt acctacggct
     7261 caagcttcat caacttcaac ctgccgcagt tctatcagat tgtgaaggtt gatggtcgtg
     7321 aacaggtgat tccgttccgc accaaatcga ttggcaacat gccgcctgcc aatcaggatc
     7381 acgacacgcc gctattacag cagtattttt cgtgatgagc gtgccggaaa gcgaggctta
     7441 tccggcatgc aatcttagcg gaaactgact gtttcacccg cttgctgcgt tgccgcctgt
     7501 tccagcaaat cccagaaggt ttcgccgcta cgatcgcaaa tccactcatc gcctttcaga
     7561 tcaaaatggt agccgccctg tttggttgcc agccatacct ggtgcagcgg ttcctggcgg
     7621 ttgataatga ttttgctccc gttttcaaag gtaatggtca gtacaccgcc gttgatttcg
     7681 cagtcgatat cgctatcgcc atcccagtcg tccagacgct cttcaatggt cagccacagt
     7741 tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg tatcctgttt ttaagtgatg
     7801 gcggcagtat agcggcatgg ggtcagagct tcaaagtttg ctcaatgtct ggtgttttgt
     7861 ggcactcttc ctccgtcgtc aagatgatga tgtcatcatt ggatgaaaac atgttctgat
     7921 ccagattacg tgtcctggcc ggacagggcg atgcggttcg ataaagttgg ctaccgatcc
     7981 aggtataaac atagcgattg tcataagcga aacgttcggt ggttttcaat ggtccatcaa
     8041 tagcaaaaat ttgaatatca tcgcgtaaac gggaagagcg cataaagtaa ccatggcctt
     8101 tcccggcgtt aatccgttgt ttcaggaaag gtgcagaaga gtcgagcgtt attatttcca
     8161 gggtgtcttc accaaaagga gacaatttcg caatgtacag atgatttttg tactgcgcga
     8221 cacgatcggt gatgggatga aatgctttcg ggtcaatatc ggggagtttc tcccattgac
     8281 tccattgctg gtctgtagag cgaagcttac gccagctaac atcggtttcg ccgacgagaa
     8341 aagagccttc gccatcagaa tattgagcaa tgttaacgga tgagttatag gggattcccg
     8401 ttggaataaa cgcaccatcc tcaagaagtc tgccgtcaag ctcaccacta cggtagtttg
     8461 cccaatagcc atatcctgaa tctttggttt tccatttcac ccgcacggct ttggtattgg
     8521 gagttgggta gcgccaaagc tgtgagccat cgaatgcgta aacataacga tcgtcgtgtg
     8581 cataccattt cttgtaatgg gggctatcgc taaggtgagc ctcatgctcg tcaggaacac
     8641 gataatccat caggataagc ggcccggaag aatcgaatac ctggaggcca tcttcttctt
     8701 caccatcagc tggtaatttg gtcaataaat agccgtgagt tcttcccgcg ttaaagcgtt
     8761 tatctatcac caaaccagga ttatccagtg tgacgatatt tagcctattt tcgccaaatc
     8821 ccgttttttt gatggtgtac agcttgtcct gatactgagc aacggtatta ctaagaggat
     8881 gaaattgttc tggatcgagg ccgggtagga cttcttgctg gcattctggc cctttgcgcc
     8941 atgaaacaga gtgttcctgc aaagtgaacg ccgcgcagct atttgcccta tctttgtcca
     9001 gatttccctg attcagcacc ttccggtgca ggccgctttt atctcgccag gttaataacg
     9061 aaccgggcat ccagcgtacg acagtaaatg tctctgggtc ggctttaagc tgttcgccat
     9121 tgattatgat tttggtccgt gtacgggcta acgtatccca ggggccaaat ggtacggcaa
     9181 cgcaggggtt aaacgtggtg gaaaactttc ctcgctgatc ccaggacttc tgcgccagca
     9241 cacggaaact ctcggggtca gcgaggcgat gtccatcgac atataagaaa ttagcgtcac
     9301 gtaatatcag ccccagaaag tcaggtttcc agggggaacg gaatttcacc tgatgcagcg
     9361 ttttaatatc tagactattt tctccgccat tgtcatcggt gcgcgtgcct tcaaaataga
     9421 gactgtgttt atctgcggca aaatcacccc atgccttgaa cgaggcgaca tcgacgggag
     9481 gtttgtctgg cgggttttgc acaacttcac ccgcgtagag gatatagcga ccatcgctat
     9541 gccaggagta atgaggatac caggcctgtg cgccttcata gaggtagtcg gttgtcagat
     9601 cagtcagagg aggtacttta tcccatttgt ctagtgttgt cgggtctatt aataaattgg
     9661 gtaaacggcg caaacttttg taattcacgt caggaagaga aagatttgtt tctttataag
     9721 ggcgcaggta taccacgcct tttccctgtt tctggtaaat attatgttca ggcgcggaaa
     9781 gccgacatcc ccatgccgac gataaaaaca acgaacaaga caacaaacaa accacattgc
     9841 gaaagtgcat aaagccatcc tggcgcgagg tgttgatcac gaaactgcta gcagaacata
     9901 aatcctcaca agtaagcgta actctcgcag caaatcgtca cttttcagaa tccataaagg
     9961 tcatcgctat tttctgatag ttgctggaat aaacgtgata ttttcattca cgatataaac
    10021 acaatcccgg aactgcccca tagtacggct aatagcataa aaatattccg gattgacccc
    10081 tggaagggag gcaatgtggg attccgcccc ccgttgcctt ctttattgtc tttcacgagt
    10141 tgtacccagg ttccaccacg ggtatctaac attcgccaca ttattacgct ttctgccgaa
    10201 cgtgattttt atagaaatct atcaattcat tttccctgta ccataaagtt tatccttcac
    10261 atcctgccga acgcgggtct tgtacccccc gttgtgaaga tcttcatacg gagaaaaaga
    10321 atctttactc caccccagca acactttatc gccaggcata aaatgcacgt gcaggtaatt
    10381 ttctccccat tctctttttg ggagaggtaa cgtcatattg cgtttctcca gacgcattcc
    10441 agcattatac tgtgtacgag tagtacttaa tgtccatata acctctgccg tatccccact
    10501 gacatcaccg caacaggtga ttgaaccgcc accattcgca aaagcattcg cgcccgccac
    10561 gccgttaaca ctgaatccca gtatcggacg gtcaatttca ctgtggattt ccagtatcac
    10621 tccacctctt ggtcctcccc atactgcctg tcctatattc cacaacacga ccagtactgg
    10681 aacgacaatt aacgtccgcc atacccagcg cccccagcgg gcataaccgc gcgtaagctt
    10741 ttggtcaaat ttttccagtt gggtaaatag ccccatcgat tttattccct cttctcacct
    10801 ggttggcaaa gttgcacgtc actggcgact aaggtcagac aacgaaattt cgtggtacgc
    10861 cactctctta aaagtcccat gcaggcgata agaacaagac aacaaacaaa ccacattgcg
    10921 atagtgcata aagccatcct ggcgcgaggt gccgatcacg aaactaccag caaaacataa
    10981 atcgccacgt gtaagcgtta tactcgcagc atttcctcac ttttcagact tcataaagag
    11041 tcgctaaacg cttgctttta cgccttctcc tgcgatgata gaaagcagaa agcgatgaac
    11101 tttacaggca atccataatg aaaaacgtgt ttaaggcact cactgtatta cttactctct
    11161 tcagcctgac gggctgcggt ctgaaaggtc cgctctattt cccgcctgca gataaaaacg
    11221 caccgccgcc gaccaaaccg gtagagatgc aaacgcaatc cacggtgccg gataaaaacg
    11281 accgcgccac tggcgatggt ccatcccagg tgaattacta aaagtcagtt tctgtacccg
    11341 cgtgattgga gtaaatgatg cagttctcga aaatgcatgg ccttggcaac gattttatgg
    11401 tcgtcgacgc ggtaacgcag aatgtctttt tttcaccgga gctgattcgt cgcctggcgg
    11461 atcggcacct gggggtaggg tttgaccagc tgctggtggt tgagccgccg tatgatcctg
    11521 aactggattt tcactatcgc attttcaatg ctgatggcag tgaagtggcg cagtgcggca
    11581 acggtgcgcg ctgctttgcc cgttttgtgc gcctgaaagg gctgactaat aagcgcgata
    11641 tccgcgtcag caccgccaac gggcggatgg ttctgaccgt caccgatgat gatctggtcc
    11701 gcgtaaatat gggcgaaccc aacttcgaac cttccgccgt gccgtttcgc gctaacaaag
    11761 cggaaaagac ctatattatg cgcgccgccg agcagacaat cttatgcggc gtggtgtcga
    11821 tgggaaatcc gcattgcgtg attcaggtcg atgatgtcga taccgcggcg gtagaaacgc
    11881 ttggtcctgt tctggaaagc cacgaacgtt tcccggagcg cgccaatatc ggttttatgc
    11941 aagtggttaa gcgcgagcat attcgtttac gcgtttatga gcgtggggca ggagaaaccc
    12001 aggcctgcgg cagcggcgcg tgtgcggcgg ttgcagtagg gattcagcaa ggtttgctgg
    12061 ccgaagaagt acgcgtggaa ctccccggcg gtcgtcttga tatcgcctgg aaaggtccgg
    12121 gtcacccgtt atatatgact ggcccggcgg tacatgtcta cgacggattt attcatctat
    12181 gaagcaacca ggggaagaac tgcaggaaac actcacggag cttgatgacc gggcggttgt
    12241 cgattatctg attaaaaatc ctgagttttt tatccgcaat gcgcgcgcag tagaagcgat
    12301 acgtgtgccg catccggtac gcggcaccgt ttcgttggtc gagtggcaca tggcccgcgc
    12361 acgtaatcac attcatgttc tggaagagaa catggcgttg ttgatggagc aggctatcgc
    12421 caacgaaggc ctgttttatc gcttactcta cttgcagcgt agtctcaccg ccgccagcag
    12481 tctcgacgat atgctgatgc gctttcaccg ctgggcgcgc gatctcggcc tggcgggtgc
    12541 gagcctgcgc ctgttcccgg attgctggcg tttaggtgcg ccgtcgaacc acactcatct
    12601 ggcattaagc cgtcagtctt tcgaaccgct gcgtattcag cgtttggggc aggaacagca
    12661 ctatcttggg ccgcttaacg gaccggagct gctggtggtg ctaccggaag cgaaagcggt
    12721 gggatcggtg gcgatgtcga tgctgggaag cgatgctgat ttgggtgtcg tgctgtttac
    12781 cagtcgcgat gccagtcact atcaacaagg gcagggaacg cagttacttc atgaaattgc
    12841 gctgatgttg ccggagcttc tggagcgttg gattgaacgc gtatgaccga tttacacacc
    12901 gatgtagaac gctacctacg ttatctgagc gtggagcgtc agcttagccc gataacccta
    12961 cttaactatc agcgtcagct tgaggcgatc atcaattttg ccagtgaaaa cggcctgcaa
    13021 agctggcagc aatgtgatgt gacgatggtg cgcaatttcg ctgtacgcag ccgccgtaaa
    13081 gggctgggag ctgcaagtct ggcgttacgg ctttctgcgc tacgtagctt ttttgactgg
    13141 ctggtcagcc agaacgaact caaagccaac ccggcgaaag gtgtttcggc accgaaagcg
    13201 ccgcgtcatc tgccgaaaaa catcgacgtc gatgatatga atcggctgct ggatattgat
    13261 atcaacgatc ccctcgccgt gcgcgatcgg gcaatgctgg aagtgatgta cggcgcgggt
    13321 ctgcgtcttt ccgaactggt ggggctcgat atcaaacacc tcgacctgga gtccggcgaa
    13381 gtgtgggtga tgggaaaagg cagcaaagag cgccgcctgc cgattggtcg caacgctgtg
    13441 gcgtggattg agcactggct tgatttgcgc gacctgtttg gtagcgaaga cgacgcgctt
    13501 tttctgtcga aactgggcaa gcgtatctcc gcgcgtaatg tgcagaaacg ctttgccgaa
    13561 tggggcatca aacaagggct gaataatcac gttcacccgc ataaattacg tcactcgttc
    13621 gctacgcata tgctggagtc gagcggcgat cttcgtggtg tgcaggagct gctgggtcat
    13681 gccaacctct ccaccacgca aatctatact catcttgatt ttcaacacct tgcctcggtg
    13741 tacgatgcgg cgcatccacg cgccaaacgg gggaaataat gcgtttttac cggcctttgg
    13801 ggcgcatctc ggcgctcacc tttgacctgg atgataccct ttacgataac cgtccggtga
    13861 ttttgcgcac cgagcgagag gcgcttacct ttgtgcaaaa ttatcatccg gcgctgcgca
    13921 gcttccagaa tgaagatctg caacgcctgc gccaggcggt acgggaagcg gaacccgaga
    13981 tttatcacga cgtgacgcgc tggcgttttc gttcgattga acaagccatg ctcgacgccg
    14041 ggctgagtgc cgaagaagcc agtgcaggcg cacacgcagc aatgatcaac tttgccaaat
    14101 ggcgcagccg aatcgatgtc ccgcagcaaa cccacgacac cttaaaacag ctggcgaaga
    14161 aatggccgct ggtggcgatc accaacggta acgcccagcc ggagctgttt ggtttagggg
    14221 attattttga gtttgtgttg cgcgctggcc cgcacgggcg ctcaaaaccg ttcagcgata
    14281 tgtacttcct ggcggcggaa aaactcaacg tgccgatcgg cgagatctta catgttgggg
    14341 acgatctcac cactgacgtg ggtggggcaa ttcgcagcgg aatgcaggcc tgttggatca
    14401 gaccggaaaa tggcgatctg atgcaaacct gggacagccg tttactgccg catctggaaa
    14461 tttcccggtt ggcatctctg acctcgctga tataatcagc aaatctgtat atatacccag
    14521 ctttttggcg gagggcgttg cgcttctccg cccaacctat ttttacgcgg cggtgccaat
    14581 ggacgtttct tacctgctcg acagccttaa tgacaaacag cgcgaagcgg tggccgcgcc
    14641 acgcagcaac cttctggtgc tggcgggcgc gggcagtggt aagacgcgcg tactggtgca
    14701 tcgtatcgcc tggctgatga gcgtggaaaa ctgctcgcca tactcgatta tggcggtgac
    14761 gtttaccaac aaagcggcgg cggagatgcg tcatcgtatc gggcaactga tgggcaccag
    14821 ccagggcggc atgtgggtcg gcaccttcca cgggctggcg caccgtctgc tgcgtgcgca
    14881 ccatatggac gccaatctgc cgcaggattt tcagatcctc gacagcgagg accagctacg
    14941 cctgctcaaa cgcctaatca aggcgatgaa cctcgacgag aagcagtggc cgccgcgcca
    15001 ggcaatgtgg tacatcaaca gccagaaaga tgaaggcctg cgtccacatc atattcaaag
    15061 ctacggtaat ccggtggagc agacctggca gaaggtgtat caggcgtatc aggaagcgtg
    15121 tgaccgcgcg ggcctggtgg acttcgccga gctgctgcta cgtgctcacg agttgtggct
    15181 taacaagccg catatccttc agcactatcg cgaacggttt accaatatcc tggtggatga
    15241 attccaggat accaacaaca ttcagtatgc gtggatccgc ctgctggcgg gcgataccgg
    15301 caaagtgatg atcgttggcg atgacgacca gtcaatctac ggctggcgtg gggcgcaggt
    15361 cgagaatatt cagcgtttcc tcaatgattt ccccggtgct gaaactatcc gtctggagca
    15421 gaactaccgc tctaccagca atattctgag cgccgctaac gccttgattg aaaacaataa
    15481 cgggcgtctg ggtaaaaaac tgtggaccga tggcgcggac ggtgagccta tttccctcta
    15541 ttgcgctttt aacgaactcg acgaagcgcg ttttgtggtt aaccgtatca agacctggca
    15601 ggacaatggc ggggcgcttg ccgagtgcgc cattctctac cgcagcaacg cccagtcgcg
    15661 tgtactggaa gaagcactgt tgcaggcgag tatgccgtac cgtatttacg gcgggatgcg
    15721 cttcttcgaa cgccaggaaa tcaaagatgc gctctcgtat ctgcgcctga ttgccaaccg
    15781 taatgacgac gcggcgtttg agcgcgtggt gaatacacca acgcggggta ttggtgaccg
    15841 gacgctggac gtggtacgtc agacatcgcg cgatcgccag ttaacactct ggcaggcatg
    15901 tcgtgaactg ttgcaggaaa aagccctcgc cggacgtgct gccagtgcct tacagcggtt
    15961 tatggaactg atcgacgcct tagcgcagga aactgccgat atgccgctgc atgtacagac
    16021 tgaccgggta attaaagact ccggcctgcg cacaatgtac gagcaggaga aaggcgaaaa
    16081 aggccagacg cgtatcgaaa acttagagga actggtaacg gcgacgcgcc agttcagcta
    16141 caacgaagaa gacgaagatt taatgccgct gcaggcgttc ctctcccatg cggcactgga
    16201 agcgggcgag gggcaggcgg atacctggca ggatgcggtg cagttgatga cgctacactc
    16261 ggcgaaaggt ctggaattcc cgcaggtgtt tatcgttggt atggaagagg gcatgttccc
    16321 aagccagatg tcgctggatg aaggcgggcg tctggaagaa gaacgccgtc tggcctacgt
    16381 tggcgtaacc cgcgcgatgc agaaactgac gctgacctac gcggaaaccc gccgtctata
    16441 tggcaaagag gtttaccatc gcccgtcgcg ctttatcggt gagttgccgg aagagtgtgt
    16501 ggaagaggtg cgcctgcgcg ccacggtaag ccgcccggtc agccatcaac ggatggggac
    16561 gccgatggtc gagaacgaca gcggctacaa gctcggccag cgcgtacgcc acgctaagtt
    16621 tggtgaaggc accatcgtca atatggaagg cagcggtgag catagccgtt tgcaggtggc
    16681 gttccagggc cagggtatta aatggctggt ggcggcttac gcccggttgg agacggtgtg
    16741 acgttgcggg atgcggtgcg ccacaccgca ttccgcatca gcaccttact tagcgcactt
    16801 cttcacatca atattccagt cagcaccaga cgcaacctga tgtttctgcg caaaattatc
    16861 catattcagc gccacggaaa cattcaataa gctgttcaga tacaccatcg gctggccttc
    16921 cggcacatcg ccgaagcttg cgacatacgg cattttccct gcgtattttt gctgggaacc
    16981 ttcggaaatg gtcacacaca acgtgtcgtt gagtttgatt cctgcctgtt tcagcaactc
    17041 gtcgctgata ttgctccaga cgttgccgta ctgaatatcg agaatcggga tattgccctt
    17101 cacttcacct tttgttgctt ttgctttctg ataagaaagt tcgacaactt ttgcaggaag
    17161 ctctggcccc acctgttcga acgtaatcgc tccggaagcc agacgcgcac cagtgtaggc
    17221 atatacatcg cgaccatgga aggtatatga cttttcagaa cctttcaggc ggttagcttt
    17281 ttcgtcgatt tcccggacgc tatcaatacc cagagtttgt gccaccaaag tgagcgtacc
    17341 gttatctggt gaaacgaagt attgaccgtt tttggttttc agcactaccg atttacggtc
    17401 agtgcctacg cccggatcga ccacgctaac aaacaccgaa ccttgcggcc agtaactggc
    17461 agtctgatac aggcgataag cgccttccca gatgttatac ggtggaattt cgtgtgttaa
    17521 atcaaagatt ttaagattat ggtcaacgcc gaatgcgacc cctttcatcg ccgagacggc
    17581 accatctttc aggctaaaat ccgtttgtaa aatcaaagcg ttatccgcat aagcggatga
    17641 tgaccccaga caggttaata aaaataatgt tgcgagcgtt tttttcattt ttgtttccct
    17701 tcatttatct gatgattttc cagctgtgta gccagcccca gtttgtctgg cctcctcgag
    17761 tgtacaatgt ttaacctttc tgctcgacgg aaatcatggt gacaaaagga tagcgttgcc
    17821 acgggattgc ctcgcctttc atatacatat gtgaaattgt gccatcaaga taaagcagct
    17881 gcccaacgtt cagtttcgct ttggcataac aggcaaaatc ataaaaattt gtcgcctgct
    17941 ggctcaacaa aaacacggcg ttcccatgtt tattaatccc aacaccgtta cgaattttac
    18001 gtgaggcgac gttgggatga atacgcggat taattacacc gttttccatc aacaatggcc
    18061 ctgactgcac cgcaaactga atctctttac tggttttaaa ggcatccaga cgaacgatgc
    18121 cgactttatc tcccgcgaca taaaacactc cgccaggacg aataaagaaa ttcccttcac
    18181 ctgaagcgag atttaacgcc accttctgct gaccgttttc gatgtacaaa ccgagcggcg
    18241 catagctttc atcatagatg ccgccgttca tcgccatctg cacctgaccc tgactattaa
    18301 tatccgccag cagagcatgt aacgttcccc acgcttcgcc attggctttt tgccagtaca
    18361 ttttcacccg ctctgtttga ggattaacgg tatacgcctg tacggtcagc gtcggatctg
    18421 agggtgcgca atcatcagcg gcaacagcaa acaagggaag taaggtgagg gtgagaaaaa
    18481 tccgtttgag attcaaggtg atcattcctt taccaatgaa tagccgatgc gccattatag
    18541 gccctggatg tgggattttt ttatcctgtt agcgaccttg acgagtacca aaaagcgcga
    18601 agttcaacta ttgttctgtg gtgttctgtt gcgtgttgac ggcaaaattt tgctggcgta
    18661 acatgcgcgc acgatcactc taagaggaca ttcgccttgg acacacccag tagatactgg
    18721 ctcactatcc tgtcatccag gatcaactcc taaggctatc cctttttgct gatagcctta
    18781 gcggttgtca gcgacctcaa tttttcccgt cgcgctgagt caggctgttt aatggtctga
    18841 aacccaattt gtttctgtgt gcccaccgaa ctgtccgata ttttaagcat tgggagtccc
    18901 ggtcatgctg agcgcatttc aactggaaaa taaccgactg acccggctgg aagtcgaaga
    18961 gtcacaaccc cttgtaaatg cagtatggat tgatcttgtc gaaccggacg acgacgagcg
    19021 actgcgcgta caatctgaac ttggtcagag cctggcaacc cgcccggaac tggaagacat
    19081 cgaagcatcg gcacgtttct ttgaagacga tgacggcctg catattcact ccttcttctt
    19141 ctttgaagat gcggaagatc acgccggtaa ctctactgtg gcatttacca tccgtgatgg
    19201 tcgtctgttt actctgcgtg agcgtgaact gcctgcattc cgtctgtatc gtatgcgcgc
    19261 ccgtagccag tcaatggtgg acggtaacgc ctacgagctg ctgttggatc tgttcgaaac
    19321 caaaatcgaa cagttggcgg atgaaattga aaacatctac agcgatctgg agcagttgag
    19381 ccgggtgatt atggaagggc atcagggcga tgagtacgac gaggcgctct ccacgctggc
    19441 ggaactggaa gatatcggct ggaaagttcg cctgtgtctg atggataccc agcgtgcgct
    19501 caacttcctg gtacgtaaag cacgcttacc gggtgggcaa ctggagcagg cgcgtgaaat
    19561 cctgcgagat atcgaatccc tgctgccgca taacgaatcc ctgttccaga aggtgaactt
    19621 cctgatgcag gcggcgatgg gctttatcaa tatcgagcag aaccgcatca tcaaaatctt
    19681 ctcggtggta tccgtggtgt tcctgccacc gacgctggtc gcttccagct acgggatgaa
    19741 ctttgagttt atgccggaac tgaagtggag cttcggctac ccaggcgcga ttatctttat
    19801 gatcctcgcg ggcctggcac cgtatctgta ctttaagcgg aagaactggt tgtaatataa
    19861 atgccgggta aagattgttc tttatccggc ttattgctga aaattgtctt tttcaaagtc
    19921 atgcgggttg aacactcgct ctatcttatt ttcctgggtt agttgatact cattgttatc
    19981 agcaataaaa aaatgagtat tacggtaagg atattcatta ataaccatta aatagttttt
    20041 tacgatgcaa ttatcattgc tcttaatcgt cgggaaacag tagagatctt catacggccc
    20101 ttcgatatat tttttatagt cagtgaagcc attaccgata tcaataacat accaggtatg
    20161 ccaactacct tctggtaggg gatgttgatt tttaatttca ttctgatgct tcagatacca
    20221 atttatttta tcgctgtcag tccatggcag atgatcaacg accagaaaga ctgaattgcc
    20281 agcatcagag tatgtataca aaatctgtac aggacgcaat gtccataaat tatagatgca
    20341 gcatagaaga aataaacata atataatttt ctggagtttg cttttcatag ataattttcg
    20401 attctttcta tagtgttaaa attggtgaaa aaaggtttga aagcaaagtc tttatgccgt
    20461 tgtaaaaaga accatatgcg aaaaaatcta tatttgctat aagtcttgtt tttgatatca
    20521 gttacatcaa gaccgaagtg gtcctgtgct acaaaatgaa ttgttgcact ccatcctata
    20581 gcgtattttt gaaaacttaa aagagatatt ttttgagctg aaatatcatg aacactgatt
    20641 cctaagccat taaaactgtc ctcgaagtca ttaaatttat ttagtcttga tgaaagtaat
    20701 tcggtcttaa tcttttgtag gagaagaggc cggtatccta tcgtaggtgt tgaagaaata
    20761 tcattttcga tacattcttt gattacacgt attgggctat tataatcata tttgtttatt
    20821 cgttcatgaa aagataagtt caattcctgg ctatggaaac tgttgccatt tccatatcta
    20881 aaatgatcaa tcagatcctg gatgagtgtt ttgtattgtc caaaaaagga aaacattttt
    20941 gccagatctt tcatttctgc aaataaaata tcaatacatt cactttgtga tatttttgtt
    21001 ccagaagttg gaacactaaa agcaccataa gtagaattcg gcccaggaaa atggtattta
    21061 atgaggcggt aaggatcaac ttttgcagag atgtcgctca agccgagtga gagaaaatct
    21121 tgttcttcca tatccccata ttgcatatca tcagcagaaa aatcatcgaa taaagtaata
    21181 gtttcgaaaa gtgtacaggg ca
//
LOCUS       DAZGTJ010000086        20684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000086 DAZGTJ010000000
VERSION     DAZGTJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.086"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            218..718
                     /gene="tssB"
                     /locus_tag="ACHK8E_003881"
     CDS             218..718
                     /gene="tssB"
                     /locus_tag="ACHK8E_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0346090.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            753..959
                     /locus_tag="ACHK8E_003882"
     CDS             753..959
                     /locus_tag="ACHK8E_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346091.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            1028..2503
                     /gene="tssC"
                     /locus_tag="ACHK8E_003883"
     CDS             1028..2503
                     /gene="tssC"
                     /locus_tag="ACHK8E_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0346092.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2510..2923
                     /gene="tssE"
                     /locus_tag="ACHK8E_003884"
     CDS             2510..2923
                     /gene="tssE"
                     /locus_tag="ACHK8E_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0346093.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2927..4777
                     /gene="tssF"
                     /locus_tag="ACHK8E_003885"
     CDS             2927..4777
                     /gene="tssF"
                     /locus_tag="ACHK8E_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0346094.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4741..5823
                     /gene="tssG"
                     /locus_tag="ACHK8E_003886"
     CDS             4741..5823
                     /gene="tssG"
                     /locus_tag="ACHK8E_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0346095.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5848..7128
                     /gene="tagH"
                     /locus_tag="ACHK8E_003887"
     CDS             5848..7128
                     /gene="tagH"
                     /locus_tag="ACHK8E_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP0346096.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHK8E_003888"
     CDS             7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHK8E_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0346097.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7652..9004
                     /gene="tssK"
                     /locus_tag="ACHK8E_003889"
     CDS             7652..9004
                     /gene="tssK"
                     /locus_tag="ACHK8E_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006688099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0346098.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDTTKKASATTISRFVATA
                     "
     gene            8947..9672
                     /gene="icmH"
                     /locus_tag="ACHK8E_003890"
                     /pseudo
     CDS             8947..9672
                     /gene="icmH"
                     /locus_tag="ACHK8E_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
     gene            9681..12464
                     /gene="tssH"
                     /locus_tag="ACHK8E_003891"
                     /gene_synonym="clpV"
     CDS             9681..12464
                     /gene="tssH"
                     /locus_tag="ACHK8E_003891"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0346099.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDA
                     QNDESVTKDETVL"
     gene            12461..13204
                     /gene="vasI"
                     /locus_tag="ACHK8E_003892"
     CDS             12461..13204
                     /gene="vasI"
                     /locus_tag="ACHK8E_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP0346100.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13209..14621
                     /gene="tssA"
                     /locus_tag="ACHK8E_003893"
     CDS             13209..14621
                     /gene="tssA"
                     /locus_tag="ACHK8E_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0346101.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14730..18164
                     /gene="tssM"
                     /locus_tag="ACHK8E_003894"
     CDS             14730..18164
                     /gene="tssM"
                     /locus_tag="ACHK8E_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP0346102.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18175..19585
                     /locus_tag="ACHK8E_003895"
                     /pseudo
     CDS             18175..19585
                     /locus_tag="ACHK8E_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19551..20030
                     /locus_tag="ACHK8E_003896"
     CDS             19551..20030
                     /locus_tag="ACHK8E_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0346103.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYK
                     IKLTGAKISDIHVSISHIVVDNSIQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20051..20269
                     /locus_tag="ACHK8E_003897"
                     /pseudo
     CDS             20051..20269
                     /locus_tag="ACHK8E_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20351..>20684
                     /locus_tag="ACHK8E_003898"
     CDS             20351..>20684
                     /locus_tag="ACHK8E_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP0346104.1"
                     /translation="MLWTENDAANTSQWNGYPLQIGRFRKDKAMPALISGEKSAALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSVIVQK"
BASE COUNT         4969 a         5606 c         5639 g         4470 t
ORIGIN      
        1 gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaga
       61 agtccttttc ttatttattt tgaagtgtag aatgcggggt gatgaaggag tggtaatttt
      121 attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa tatgccgtgt
      181 tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag
      241 cgtcgcgcct cgcgagcgta taaatatatc ctatgtcccg aagacggacg gccagacggc
      301 tgaagtagag cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggagac
      361 atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtaat
      421 ggctgaagca gccattggcc tcaattttac cgtccccgcc acgctgaagg gaagcacaac
      481 ggacgatgaa ctgaatgtgg cgctgaatat caagtcactg gatgattttt caccggacag
      541 cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc
      601 gctgaagggg ccgatgggca accttccggc attccgtacc cagctccagg cattgctgga
      661 aaatgaggag tcccgcgagc agttgcttaa agaaatcggc caggtgagtg ggaaataacc
      721 accactattt cattaaacag ggttatccag acatggcggc acacaaaaat atctccggga
      781 aacaccccct ctctgacgaa gacattttgc aggcggttta ccgggtatgt ttcagcccgt
      841 ccggtgaaat caaccgggat ggcgtggatg cgctgattac aacactggat cgccgtatca
      901 gtgatctatt ggatgagatt atccatcttt gtgagtttca gcagagcgcg ccgtactgac
      961 ggcgcgtact tcactgacgg cgaaaacccg gtttcagaaa cacagctaaa acagaaggat
     1021 tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc agactgatca
     1081 ggcgtcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcctg gttcggaagg
     1141 ttatgacgtt gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc
     1201 atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata tagatgaacg
     1261 catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt
     1321 ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa
     1381 cgtgcttcat gtcacgaagc aggaacttct ggaagacttt gaatttgccc cagaaattat
     1441 tcagtccgga ttttataaac acgtttattc gtccggattc ggacagtttg gtggtgaacc
     1501 catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gctcctgaca tgaaactgct
     1561 gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc
     1621 ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat
     1681 ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta
     1741 tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc
     1801 tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg
     1861 taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg
     1921 tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt
     1981 cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg
     2041 cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa
     2101 tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagacgc
     2161 agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc
     2221 gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt
     2281 agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc
     2341 agacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg
     2401 cgaacccggc tggtaccagg ttgcgctaag cgtgaggcct cattttaaat ttatgggggc
     2461 aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga
     2521 tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcag ccagaaaccg
     2581 gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct
     2641 taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg
     2701 ggctgaaaac ttccgcgaat caatgagtcg ggccatcgag caatgtatag aacgctatga
     2761 accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga
     2821 tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt
     2881 tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga
     2941 acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga
     3001 aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata ttgaacgcct
     3061 gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc
     3121 agaactgacg cacgggctta ttaagatgct ctggcctaat tacctgcgtc cggttccggc
     3181 aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc
     3241 ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc
     3301 tgatgctaaa aaggaggagc ctcctccctg taccttcacc ctctgccggg atatctggct
     3361 gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa
     3421 catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg
     3481 cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga
     3541 atacttgcag ggtgctgacc tgactgtggg tgaacagcat cttcgcctgc ctgagtttat
     3601 gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca
     3661 cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga
     3721 tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact
     3781 gcgtttttcg cacccattgc ccgtggacat ccggttgcgc cgcgactccc tgcgcctgta
     3841 ttgcgcgcct gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg
     3901 gcgggcagac tatccgctgg ttcccagccg ccattatccg gaacattacg atgtcttttc
     3961 cgttaacggc gtgataagcc aggtgcagga tatgttcagg aaaaaagatc tgggtcgtcc
     4021 tgtttcgacg caggccgcgc gccagtggcc tgcctttgaa agtttcagcc atcagatgga
     4081 atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca
     4141 tcgcggcttt gatcataccc ttgcttttat acatgctgat ggcagttatc cgtcagacga
     4201 atcgctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgaacttcc
     4261 gtcacaaatt cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc
     4321 atttcgcaac attacccgcc cgacgcaacc gctctggccg gtcattgatg gcagcctgca
     4381 ctggtccctg ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct
     4441 gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga cggcgcggct
     4501 gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccctgtcg atcgcctgtt
     4561 tacgggcatt cccgtccggg ggctggcctc cacgctgtat ctgcacccgg agccgtttgt
     4621 ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc
     4681 cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa
     4741 atggacggaa agaaccgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat
     4801 aaagagcaga atgatccgct ggcacaggtt tttcataacg cctgctctta caattttttt
     4861 gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc
     4921 ctgcgtgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc
     4981 ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag gatgaccacc
     5041 acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg
     5101 gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac
     5161 cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt
     5221 aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc
     5281 cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg
     5341 gcaaatccgg gacgatcccc ggaaataatc tgcggcctgg tgtcccactg ctttgatttg
     5401 tcagacgtga cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa
     5461 aacagtctgg ggagctattc acttaaaaat ggcgaaaaac tggcgggaag atcggtactg
     5521 ggcaactttg ttctgggtac acgggtgccc gatctcagcg gaaaattcca gctcagtatt
     5581 accagtctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca
     5641 ctgacgatgt ttgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg
     5701 ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc
     5761 tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg
     5821 taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg
     5881 caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtgt gttcacacag
     5941 cagggaaatg tcggccatgg ccccgaatgc caatggtcgg tacaggatcg tcagcagagc
     6001 attccggctc aggcttttac cgttatcctg cacgatggca cattttgtct gcgcccgcag
     6061 acggcacaac tgtggctgaa ccaggcaaaa gtcacagcaa catcagacct gatacagctg
     6121 cgccagggcg atgagatcca gatagggcgt ctgatggtga gggttcatct gaaccgggga
     6181 gatatccccc attacgatga ggaaatggcc actcccgaaa ccatcgtcac caatcgcgat
     6241 atgctcacgg ataccctgct gtcaacggag ggtgcgccac actatccggg aatgactcac
     6301 cggcatcagc ttgcagacac cgtggtaaac ggtttttcta ccgatccact ccaggcactt
     6361 cagtccgaaa gcctgattac cgcgggcgat ccgctttcag gcattgcggc tgtccggcca
     6421 tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg
     6481 ccgcccattt atgccagccc cggcgatcgt aatgatgacg tctctgtggc agaaatggcg
     6541 caacgccacc ttgcggtcac ccccttactg cgcggtctcg gcggttcgct taccgtgagc
     6601 aattccgacg atgcggatga ttttctggaa gaggccggac gcacgttaca ggccgcaata
     6661 aaaggtctgc tcgatttgca gcaacagcgt aacagcctct cagacaaaca tttgcgcccg
     6721 ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtgatgttt
     6781 gccgaaggta aaagtccggt acatctggcg gctcccgccg ctgtcagtga aagcctgcgc
     6841 aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcact tcgtgtcctg
     6901 cttgacgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat
     6961 gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa
     7021 gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag
     7081 gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt
     7141 ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca
     7201 gcgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa
     7261 taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggagggt gaatcagccg
     7321 ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg
     7381 accagatagc cagtacgccg ctaccggatg tgttgggtaa aaactatatc gaccaccagg
     7441 atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa
     7501 cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa
     7561 tcgagacggt ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca
     7621 gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg
     7681 gcaggaaggg atgctgatgc gcccacacca tttccagcaa cagcagcgtt acaacgacta
     7741 cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac
     7801 cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt caggcatact
     7861 gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc
     7921 gcaaaatttt ccggacgaaa gaagccgcaa tatttacctt gctctgccgg tcgccagtga
     7981 tgtgagaaat gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga
     8041 tgtccgggat ctgcattcag aagaaggtga cgcgcgaaca ctgacgctgg gtcaactgac
     8101 gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc cgctttgccg
     8161 tatcagcgat cgccatgccg acggctccct gacgctggat gacgatttta tcccctcctg
     8221 ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac aaggggccat
     8281 tggcggacgg gcaagcgatc tggcaaaccg cattggctct ccggcgcaga gcggcatcgc
     8341 ggatgtggcg gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca
     8401 tcgcgctcgt cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct
     8461 gggtgaactg atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga
     8521 tcatcatgac ctgaccaaaa catttaaaac gctgttaccg gaagtcaaac gggcgctgca
     8581 taccgtactg tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca
     8641 ggccgatatt catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc
     8701 aaacatgccg gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc
     8761 ggaaaaaatc cgcaatatgg tcagtgtgca gatacccggc attccattgc gtgccctgat
     8821 ggtggctccc cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaaaag
     8881 cggccaggcc tgggctgaaa tagctgccgc cggggccgtt gcactgcatg tttccggcag
     8941 tttcccggac acgacgaaaa aagccagcgc gactaccatc tcgcgcttcg tggcaacagc
     9001 ctgaatccga tgatcgacgc ggccacgccg ctactcggta tggtaatgcg cctttcaacc
     9061 atgaacagcc agacgatgcc ggagcatctt tttgctcagg tggtgacgga tgttcaggcc
     9121 gtggagcaac tgcttcagga gcagggctac gaaccgggcg tgattatctc gtttcgctac
     9181 atcctctgca cgtttattga tgaagcggcg ctcggtaacg gctggtcgaa caagaacgag
     9241 tggatcaaac agtcattgct ggtccatttc cacaacgaag cctggggtgg agagaaagtt
     9301 ttcatcctgc tggagcgcct gatccgtgaa cccaaacgtt accaggatct gctggaattt
     9361 ctctggctct gtttttctct gggatttcgc gggcgctaca aagtggcagc acaggatcag
     9421 ggggaatttg agcagatcta ccgccgcctg taccacgtcc tgcataaact acgcggcgat
     9481 gcccctttcc cgctgttaca ccaagacaaa aaaacccagg gcgggcgtta tcagctcatc
     9541 agtcgcctga ccattaaaca tattttttat ggtggcgttg tcgtgctggc gttgttttac
     9601 ctgttctatc tgttgcggct ggacagccag acgcaggaca tcctgcacca gctaaacaaa
     9661 ttacttcgat aaggacgttt atgatccaga ttgatcttcc cacgctggta aaacggctta
     9721 acctgttctc ccgccaggcg ctggagatgg ccgcctctga atgtatgagc cagcaggcag
     9781 cggaaattac cgtcagccat gtgcttattc agatgctcgc catgccacgc agtgacctgc
     9841 gggttattac ccgccagggc gatattggca tggaagagtt gcgccaggcg ctgacggtgg
     9901 agaactacac aactgcccgt tctgcggaca gctacccggc gttttccccg atgctggttg
     9961 agtggctgaa agagggctgg ctgctggcgt cggctgagat gcagcacagc gaattgcgtg
    10021 gcggcgtgtt actgctggcc ctgctgcatt cgccgctgcg ttatataccg cctgctgccg
    10081 cccggctgtt gaccggcatt aaccgtgacc gtctgcaaca ggactttgta cagtggacac
    10141 aggagtcggc ggaatcagtc gtgccggatg cagacggtaa aggcgcaggc acactgacgg
    10201 acgctgctga caccctgctt gcccgctacg ccaaaaacat gaccgcagac gcccgtaacg
    10261 gcaggcttga cccggtactg tgccgcgacc acgaaatcaa cctgatgatc gacattctct
    10321 gccgccgccg caagaacaac ccggtggtgg tgggcgaagc aggcgtgggt aaaagtgcct
    10381 taatcgaagg gctggcgctg cgcatcgtgg caggccaggt gccggacaaa ctgaaaaaca
    10441 ccgatatcat gacccttgac ctgggcgcat tgcaggccgg ggcgtcggtg aagggcgaat
    10501 tcgaaaaacg cttcaagggg ctgatggcgg aggtcatttc ctctccggtg ccggtcattc
    10561 tgtttattga cgaagcacat accctgattg gcgcgggcaa ccagcagggc gggctggata
    10621 tctccaacct gctcaaaccg gcgctggcgc gcggcgaact gaaaaccatc gccgccacca
    10681 cctggagcga atacaaaaaa tacttcgaga aagatgccgc tctgtcgcgc cgcttccagt
    10741 tagtgaaggt cagcgaaccc aacgccgctg aagccaccat tattctgcgt ggtctgtcgg
    10801 cggtctatga acagtctcac ggcgtgctga ttgatgatga cgccttgcag gccgcagcaa
    10861 cgctgagcga acgttatctc tccgggcgtc agttgccgga caaagccatt gatgtgctgg
    10921 ataccgcctg cgcccgcgtc gccattaacc tttcctcgcc gccgaagcaa atctcggcgc
    10981 tgaccactct gagccaccag caggaggcgg aaattcgcca gcttgagcgc gagcttcgca
    11041 tcgggctgcg taccgacaca tcacggatga ccgaggtgct ggtgcagtat gatgaaacgc
    11101 tgacggcgct ggatgaactg gaggccgcct ggcatcagca gcaggcgctg gtacaggaga
    11161 ttatcgcgct gcgccagcaa ttgctgggcg tggcagagga cgatgcggcg ccgttgccgg
    11221 acgcagatac cgtggaggat acgccgccag agtcagagtc agagtcagag tcagagtcag
    11281 aacaggataa taccggtgcc gtaccggctg atgaggccga cagagaacag ccggaagaga
    11341 ccgctgaaac agtttccccg gtacagcggc tggcccagct cactgccgaa ctggacgccc
    11401 tgcataacga ccggttgctg gtctccccgc acgtcgataa aaaacagatt gcggcggtga
    11461 ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca gaatgaaatg tcggtcatca
    11521 ccgacctgcc gaaatggctg ggcgacacca tcaaaggcca ggacctggcg attgccagcc
    11581 tgcataagca tctactgacc gcacgcgcag acctgcgtcg tccgggacgc ccgctcggtg
    11641 cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga aaccgtcctg caactggcag
    11701 agctgctcta cggcggtcgc cagtacctga ccaccatcaa tatgtccgaa ttccaggaga
    11761 aacacaccgt ctcgcggctg attggttcgc ctccgggcta cgttggctac ggtgaaggcg
    11821 gcgtactgac cgaagcgatt cgccagaaac cctactcggt agtactgctc gatgaagtgg
    11881 aaaaagcgca cccggatgtg ctcaacctgt tctaccagtc gttcgataag ggcgaaatgg
    11941 cagacggtga aggccgcctg attgactgca aaaatatcgt cttcttcctg acgtccaacc
    12001 tcggctacca ggtaatagtc gagcacgccg atgacccgga aaccatgcag gaagaactgt
    12061 atccggtgct ggccgacttc ttcaaacctg ccctgctggc gcgtatggaa gtggtgccgt
    12121 atctgccgct gtcgaaagag acgctcgcca ccattatcgc cggaaaactg gcccgtctgg
    12181 ataacgtgct gcgcagtcgc tttggtgcag aagtggtcat tgaaccggaa gtgacggacg
    12241 aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag gatgctggaa tcggtcatcg
    12301 atggcgacat gctaccgccg ctctcgctgc tgctgttgca gaaaatggcg gctaacacgg
    12361 cgattgcccg gattcggttg tcggcggtgg acgacgcatt tacggcagac gtggaagatg
    12421 ctcagaacga cgagtccgtc acaaaggatg aaacggtttt atgagcaggt tgcgcgtttt
    12481 ccggcagaca ggatggttta tcgcgggcct gatgacgggc ttacccgcca ctgccgcgcc
    12541 cgcagaaaca tcgtcaatgg ccggtgtggc ggtcgccgtc gccaccacgc ccccgccgga
    12601 tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg gagtccgcgg cgctggagcg
    12661 gcttgactgc tatgaccgcc tgctggcacc gctgtccccg tcagggtttg acggggcgct
    12721 ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc acagagcagg aaaaacgccg
    12781 cgagggcaac accacagaac tgctggtgac gcaggtgccg ggggagcgcc cgacggtggt
    12841 gataaccacc ccggctatcg gccatgttcc gccgcgcccg gtgctgatgt tcagttgcgt
    12901 ggacaatatc acccggatgc aggtggcgct gatgcaccct ctggatgtcc atgatatcgc
    12961 cgtcactctg aatgccgaca gccgggcatt gcgtagccac tggtttgtgc gcgagaacgg
    13021 caccctgctg gagtccagcc gggggctgtc cgggattgat gaaattaagc aactgttcgg
    13081 cgcaaaaacg ctgacagtgg ataccggcgc agataacgcc gcaggaaagc tgacctttaa
    13141 cattgacggg ctggcaaggg cgattgcccc cctgcgcgtt gcctgccact gggcgggaga
    13201 ataaaaatat ggcaatgaac ttacgcgatc cgaatgtctg gatatcgcac ctgctggaaa
    13261 acctgccgga agaaaaactg gcatcggcgc tgaaagatga caacccggac tgggagtata
    13321 tcgacggcga aatcgtcaaa ctgggatctc tggcccacag tcaactggat atcccggaac
    13381 tccagcgccg ggggctggtc attctggctt cggagagtaa ggactttcgt ctgctggcgc
    13441 atctgctgag aaccctgcaa cacgccggtg atcctcatct cgcattgcgc ctgctggcgc
    13501 tgtatgtgga gcattactgg tcggtggctg cgccgcagaa tatggcacat aaaaaacgct
    13561 ttgccagcca ggtcattaaa cgctttgaaa caggtattga aggcttttca cagaacgccg
    13621 ccacagcgca gcacgacgcg ctgtcagggg aactggcgaa actggcgcag tgctggcagt
    13681 catataacgc cccggaactg gcactggcta ccgatgatct ttttgccctg taccagcgtg
    13741 cgtttaatcg tgcggcaccg gctcctgccc ccactccggc ggcctccgat agttcacgac
    13801 agaccaccgc cacgtctgaa agcggcgtga cgcaacccag tgctccggct ccccaaatcg
    13861 ccatcgacag tcacgacgac aaagcctggc gcgacacgct gttaaaagtg gcggctattt
    13921 tatgtgaacg ccagccggac tcgccgcagg gctatcgcct gcgccgccat gccctgtggc
    13981 aatccatcac cagtacaccc caggcggaaa gcgatggacg taccccattg gctacggtct
    14041 ctgccgatat ggtggcggat taccagtccc ggcttgccag cgcggatatg gcgctgtggc
    14101 agcaggttga gaaaagcgta ttgctggctc cttactggct ggacggtcac tgtctttctg
    14161 cacagacggc actgcgtctg ggttacaaac aggtggcaga caccatccgc gatgaggtca
    14221 tccgcttcct tgagcgtctg ccccagctta ccgggctgct gtttaatgac cgcacaccgt
    14281 ttctcagtga gcagacgaaa caatggctgg cagcttcgcc cgacggcaaa gttgcaccgg
    14341 ttgcgcaaat cggtgaggaa tcgcaggcag ccagagcctg ttttgctggg cagggtctgg
    14401 aggcggcgct gcgatatctg gacatgctac ccgaaggcga tccccgcgat cagtttcacc
    14461 gccagtacct tgccgcacag ttgacggagg aggcggggct gatacagctt gcgcagcaac
    14521 agtaccggat gttgttgatg atagggagtc agatgatggt gtctgactgg gagccatcat
    14581 tacttacgca gcttgaacaa aaattcacgg cagaacaata aaagagaaca ggagtttttg
    14641 tgttcagatt accaacaccc cgactactca gcggactcaa atcagcccta cgcccggcga
    14701 tgccccggtt taaagtctcc gctttctggc tgctgatact ggcgtggatt tttctgctgg
    14761 tatggatctg gtggaaaggc ccgacatgga cgctgtatga agaacagtgg ctcaaaccac
    14821 tggcgaaccg ctggctggca acggcggcat gggggattat cgccctagtg tggcttaccg
    14881 tccgggtgat gaagcgcctg caacagcttg aaaaaatgca aaagcagcag cgcgaggagg
    14941 ccattgatcc gctcagtgtg gagctgaacg cccagcagcg ttaccttgac cgctggctgc
    15001 tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg gcagttgccg tggtatatgg
    15061 tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg cgagggtttt ccgtccgaca
    15121 ttatttatgc cccggagggc gcacgcggcg cagaacaacg cctgtacctc acgccccatg
    15181 tcggtaaaca ggcggtgatc tttgatatcg acggcacact ctgcgctccc gctgatgcgg
    15241 atatcctgca tcgccgtctg tgggaacatg ccctcggctg gctgaaagaa aagcgcgcgc
    15301 gccagccgct gaacgggatt attctgacac tggatttacc cgatctgctt accgcagaca
    15361 aacgccgccg cgagcatctg ttacagacgc tgcgcagccg cttgcaggat atacgccagc
    15421 atcttcactg ccagttaccg gtttacgtgg tactcacccg gcttgattta ttgcagggtt
    15481 ttgccgccct gttccagtca ctgaacagac aggatcgcga tgcaattcta ggggtcacgt
    15541 tcacccgccg cgcccatgaa aatgatgact ggcgaacaga gttgaatgct ttctggcaga
    15601 catgggtgga tcgaatgaac ctagcgttgc cggatctgat ggtcgctcag actcacaccc
    15661 gcgcgtcttt atttagtttt tcccgccaga tgcagggaag ccgtgaaccg ctggtgtcac
    15721 tgcttgaggg tctgcttgac ggcgaaaata tgaacgtgat gctgcgtggt atctatctca
    15781 cctcttcgct tcaacgcgga cagatggatg atattttcac ccagtctgcc gcccgccagt
    15841 accggctggg caataaccca ctggcgtcct ggcccctggt ggacaccgcg ccttatttca
    15901 cccgtggcct gttcccgcag gcattactcg cagagcctaa tctggcaaca gaaagccgca
    15961 cctggctgat gcgttcccgt cgccgcctaa cggttttctc taccacagga ggcgtggcgg
    16021 cactgctgct catcaccggc tggcatcact attacaacgg taactatcag tccggcatca
    16081 ccgtgcttaa gcaggcaaaa gcctttatgg atgtaccgcc tccgctgggg gaagatgatt
    16141 acggcaacct gcaactgccg ctgctgaacc cggtacgcga tgccacgctg gcctatggcg
    16201 actggggcga ccgcagccgt ctggccgata tgggactgta ccagggacgg cgtatcgggc
    16261 cttatgtgga acaaacctat ctgcaactgc tggagcagcg ttacctgcct tcgctgttta
    16321 acgggctggt caaagcgatg aacgccgcgc cgccggagag tgaggaaaaa ctcgccgtgc
    16381 tgcgtgtgat gcgaatgctg gaggacaaaa gcggacgcaa taatgaggtg gtgaagcagt
    16441 atatggcaaa acgctggagc gaaaaattcc acggccagcg cgatatccag gcacaactga
    16501 tgtcccatct tgactacgcg ctggctcata ctgactggca tgcagagcgt caggcgggcg
    16561 acggtgacgc catcagccgc tggacgccat atgacaagct cgtggtatca gcacagaaag
    16621 aactgagcaa actgcctgtc taccagcggg tttatcagag cctgaaaacg cgggcgctgg
    16681 gcgttcttcc tgctgacctc aatctgcgtg accaggtagg gccaaccttt gaccaggtgt
    16741 ttacatctgc cgatgacaac aaactggttg ttccacagtt tatcacccgc tatggcctgc
    16801 aaagctattt tgtaaaacag cgcgatgaac tggttgaact gacagcgatg gattcctggg
    16861 tacttaacct cacccgcagt gtgaaataca gtgacgccga ccgtgcggaa atccagcacc
    16921 agttgaccga gcagtatatc agcgactaca ccgccacctg gcgggccggg atggacaatc
    16981 tgaatatccg caattttgag tccatcggac aactgaccgg ggcgctggag caggttatca
    17041 gcggcgacca gcctttgcag cgggcgctga ccgtgctgcg tgacaacaca cagccaggcg
    17101 tcttttctga aaaactctct gccaaagaac gggaggaagc cctggcagag ccggattacc
    17161 agttactcac ccgcctcggg catgaattcg ccccggaaaa cagtaccctg gcagtacaga
    17221 aagacaaaga aagcacgatg caggccgtgt atcagcaact caccgagttg caccgctacc
    17281 tgctggcaat ccagaacgcg cctgtaccag ggaaatcggc gctgaaagcc gtgcagttac
    17341 ggcttgatca gaacagcagc gatccgatat tcgccacccg ccagatggca aaaacgctgc
    17401 ctgctccgct caaccgctgg gttggcagac tgactgacca ggcctggcat gtggtgatgg
    17461 tggaggctgt tcattatatg gaagtggact ggcgcgacag cgtggtgaaa ccgtttaacg
    17521 agcaactggc aaataactat ccgtttaatc cgcgttctgc acaggatgcc tcactggatg
    17581 ccttcgaacg cttctttaaa ccggatggca tactggatac cttctaccag cagaacctga
    17641 agctgtttat cgataatgac ctgagtctgg aggatggcga taacaacgtc attattcgcg
    17701 aagatattat tgcgcaactg gaaactgcgc agaaaatccg tgacatcttc ttcagcaaac
    17761 agaacggtct gggaacatcc tttgccgtgg aaacggtatc gctttccggc aataaacgcc
    17821 gcagtgtact gaaccttgac ggtcagttag tcgattacag ccagggccgt aactataccg
    17881 cccatctggt ctggcctaac aacatgcgcg aaggcaacga aagtaagctg acgctcatcg
    17941 gcaccagcgg caacgcgccg cgcagtatca gcttcagcgg gccgtgggcg cagttccgcc
    18001 tgttcggggc cggacaactg accggagtac aggatggcaa ctttaccgtg cgctttagcg
    18061 tggacggtgg cgcgatgacc taccgtgtgc ataccgacac cgaggataac ccgttcagcg
    18121 gtgggttgtt cagccagttt ggtctgtcag acacactgta ctgaggaggc agggatgaat
    18181 agtaacgtac tgacacaaac tatcgttacc ggcagtgacc cgcgcgggct gccggaattc
    18241 agcgccattc gcgaggaaat aaacaaagcc agccacccgt cacagcctga gctgaactgg
    18301 aaactggtgg agtcgctggc gctggcgatt tttaaagcta acggtgtgga tttacacacc
    18361 gccacctact atacgcttgc acggacacgg acacagggac tggcgggctt ttgcgaaggt
    18421 gcggaactgc tggcggcaat ggtaagccac gactgggata agttctggcc acagggcggt
    18481 ccggcgcgta ctgaaatgct ggactggttt aactcccgaa ccggcaatat tctgcgtcag
    18541 caaatctcct ttgcggaatc cgacctacca ctgatctacc gcacagagcg ggcattgcag
    18601 cttatctgcg acaagctcca gcaggtagag ctgaagcgcg ttccgcgcgt ggagaatcta
    18661 ctctatttta tgcagaacac gcgtaaacgg cttgaacccc agccgaagag taacgcagag
    18721 aacgccgcac agaccacggt cagaacgctg atttatgccc cggaaacaat ggcttcctcc
    18781 acgccagaag cggtagtgcc tcccctgccc gacctgcctg agatgaaagt ggaagtgcgc
    18841 agtctgtcag aaaatccccc acaggccagt gtgatgaagc aaggcagtac ggtaagaggg
    18901 tttatcgcag ggatcgtctg ttcagtggct atcgcctcag cattgtggtg gtggcaggtc
    18961 tatccggcgc agcagcaact gttacaggtt aacgacaccg cgcagggcgc agcaacggtg
    19021 tggatggcct cacctgaact tgaaaactat gagcgcaggc tgcatcagct tcttggcgtc
    19081 tccccggtac aaccgctgga aaccgggatg cagatgatgc gtgttgccga cagtcgctgg
    19141 ccggaaagcc tgcaacagca gcaggcctcg acacaatgga atgaggcact caaaacccgc
    19201 gcacagagta gcccacagtt gcgcggctgg ttgcagaccc gccaggactt acatgctttt
    19261 gctgatctcg tgatgcagcg cgagaaagag ggattaaccc tttcctatat caaaaatgtc
    19321 atctggcagg cggagcgggg actggggcag gaaacacctc tggaatccct gctgacgcag
    19381 taccaggatg cccgtgcgca gaagcagaat acagatgcgc tggaaaaaca gattaacgag
    19441 cggatggacg gcgtgttaag tcgctggctg ctgctgaaga ataatacgat accgacgata
    19501 aaaaaagaca ttgaatttca ataacattca tgaatacaaa ggagttttaa atggcgaatt
    19561 taatttattt aacactaaat ggtgataatc aagggctcat ttcaagtggt tgttcatcac
    19621 aaccatccat tggaaataaa gctcaaacag cacacattga tcaaattatg atttatgaat
    19681 tcatgcatgg attaaatcga gatcaaaacg tcaaccatca tcatttgaca ataaaaaaac
    19741 ctatagataa agcatcaccc ttattaggca aagcaatttg cgacaatgaa ttattgacat
    19801 gtgacttttc cttttatcgt acaaataaat ttggaattaa tgaactgtac tataaaatca
    19861 aattaactgg agcaaagata agcgacattc atgtctctat atcacatatt gttgttgata
    19921 acagcattca acccgaagag tcagtttctt tcagttatga gtcaattata tgggagcatt
    19981 gttccgctgg cactagtgca tacagtttat gggaagatcg tcttttttaa aaatatctgg
    20041 gattattata atggctaaca atattttaca ctcttcaaca tcagacagtg aaagcaaaaa
    20101 tacaagcact gacagtacgt tatttaatga aaaattccca tttcaactaa ggcaagattt
    20161 cgcatcaaga ataacagaag atgaaagtga tttttttaaa tggaaaaaaa taagagagtt
    20221 ggcatttgtg agcaatcata aagaatggaa taaatatgac ttattaattt taaaatctgt
    20281 taatgaaatc aacattcatt tgtccagcac tccatacttt cagccgttgg attggtatat
    20341 aataaaagcc atgctttgga ctgaaaacga tgcggcaaat acttctcagt ggaatggtta
    20401 tccattacaa attggtcgtt ttcggaaaga taaagcgatg cctgctttaa tctctggaga
    20461 aaaatccgca gcccttgtta ccccaccaca atggagaaac aaagcattca atgggttaaa
    20521 agatccagaa agaaattact gggcaaaaga acaaataact ggctctccag aagagaatat
    20581 caaagcagca attacttact taatgatgaa actttctaat acaaaggaag aatcaactat
    20641 cgatcaatat gactcaactc tatatagcgt catcgtccaa aaag
//
LOCUS       DAZGTJ010000087        20032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000087 DAZGTJ010000000
VERSION     DAZGTJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.087"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            307..870
                     /gene="ipbA"
                     /locus_tag="ACHK8E_003899"
                     /gene_synonym="fimX"
     CDS             307..870
                     /gene="ipbA"
                     /locus_tag="ACHK8E_003899"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFP0346105.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
     gene            1200..1994
                     /gene="hyxR"
                     /locus_tag="ACHK8E_003900"
     CDS             1200..1994
                     /gene="hyxR"
                     /locus_tag="ACHK8E_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFP0346106.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            2148..2909
                     /gene="hyxA"
                     /locus_tag="ACHK8E_003901"
     CDS             2148..2909
                     /gene="hyxA"
                     /locus_tag="ACHK8E_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP0346107.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            2951..5533
                     /locus_tag="ACHK8E_003902"
     CDS             2951..5533
                     /locus_tag="ACHK8E_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0346108.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAYAYAGVYADDDKASSNLNINLS
                     DGSVWKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTG
                     DVGNAKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGAS
                     TVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGS
                     LDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVI
                     QAMAAAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDV
                     NGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIF
                     IDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKL
                     SALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSD
                     GNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGI
                     NVTW"
     gene            5715..6383
                     /gene="ykgH"
                     /locus_tag="ACHK8E_003903"
     CDS             5715..6383
                     /gene="ykgH"
                     /locus_tag="ACHK8E_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP0346109.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTRPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(6624..7319)
                     /locus_tag="ACHK8E_003904"
     CDS             complement(6624..7319)
                     /locus_tag="ACHK8E_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0346110.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7312..8739)
                     /locus_tag="ACHK8E_003905"
     CDS             complement(7312..8739)
                     /locus_tag="ACHK8E_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0346111.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(8750..9469)
                     /locus_tag="ACHK8E_003906"
     CDS             complement(8750..9469)
                     /locus_tag="ACHK8E_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0346112.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
     gene            complement(9997..10851)
                     /gene="rclR"
                     /locus_tag="ACHK8E_003907"
     CDS             complement(9997..10851)
                     /gene="rclR"
                     /locus_tag="ACHK8E_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP0346113.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11077..12402
                     /gene="rclA"
                     /locus_tag="ACHK8E_003908"
     CDS             11077..12402
                     /gene="rclA"
                     /locus_tag="ACHK8E_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP0346114.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            12511..12747
                     /gene="rclB"
                     /locus_tag="ACHK8E_003909"
     CDS             12511..12747
                     /gene="rclB"
                     /locus_tag="ACHK8E_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP0346115.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            12759..13352
                     /gene="rclC"
                     /locus_tag="ACHK8E_003910"
     CDS             12759..13352
                     /gene="rclC"
                     /locus_tag="ACHK8E_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP0346116.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(13512..14381)
                     /locus_tag="ACHK8E_003911"
     CDS             complement(13512..14381)
                     /locus_tag="ACHK8E_003911"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0346117.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            14630..15487
                     /locus_tag="ACHK8E_003912"
     CDS             14630..15487
                     /locus_tag="ACHK8E_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0346118.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(15612..19862)
                     /gene="fdeC"
                     /locus_tag="ACHK8E_003913"
     CDS             complement(15612..19862)
                     /gene="fdeC"
                     /locus_tag="ACHK8E_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP0346119.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         5362 a         4803 c         4474 g         5393 t
ORIGIN      
        1 aaaataacat taaataaatc aagactattg aacatcacta aaacattaga cgtaatacac
       61 attcaccccc gctccttgac tcagggtttt aatatttttt gtttcacatc attgtagaaa
      121 aaatcgcgca ttaaaaatga gttgtagaaa aaccgcacaa taaatatttc atatttaatc
      181 tattgcgtag aactactatt caaaaaatct tattaatgag ataatgaaat tacatcgata
      241 acgttctgat tgcaggcaca cttatctggg tgtgcctctt ttttttacaa ctgtacacgg
      301 tggcgtatga cgagaaaata tctcacacag gatgaagtct acaggctgat ggatgcagcc
      361 cagagcatgt cctttcctga aagaaatcgc tgtctaatta tgatggcttt cattcacggt
      421 tttagggcca gtgaacttct tgatttacgt ttatccgata ttgatgcatc aggaaaacaa
      481 cttaatatcc ggcgtattaa aaatggcttc tcgacaacac atcctcttct tcctgatgaa
      541 tataatttga tcaagttgtg gcttaagcag aggaagttaa ttgaaaatgg agttgaagga
      601 gactggcttt ttctctcccg gaaatgccgc cctatcagca gacaacattt tttttcgatc
      661 attcgtgagg caggaagacg tgcaggatta gctgtaaaag cacatcctca tatgttacgt
      721 catgcctgtg gatttgctct ggctgacaat ggtgtcgata cccgactact tcaggattat
      781 ctgggtcatc gaaatattca gcatactgtc agatacacag ccagtaatgc tgctcgattt
      841 aaaggggtgt ggaaaaaaaa gcctcgttaa aacagtacct tctgattcaa aatgtcagat
      901 tcctacgttt gcctcaattc ttccataaaa aaacagctca ttcaattaaa taaaacacat
      961 aacatgttga tatttctcgc taaagtaaaa taaccccatg atttgaaatg attaaccaca
     1021 aaggtcatac cagtgatcag ttttcactcg cgttgttaaa taacacaaaa ttaaaaacaa
     1081 taaaaatcaa acaaattaga tttatattcc aataaaatta aatattcagg caattaaacc
     1141 aaacctgaca ttttgaatca aaatgtcagg ttttgtagca atggcgtgga cgagtctgaa
     1201 tgaagaagcc tctggttatt atcagtgcct gtcagttcac caggctggca ctggagtctc
     1261 tcatcccaac ggacaggtat atcgtgcggg tgtacagcaa cgtcactaca gaggttgaat
     1321 tcgttttaaa gacctcctgt ggctacctgt tagctgatat cccatcgctt ccttcgggag
     1381 agttggttct tctggcttgt cttgtccgct tgcggagtga tgccgggcaa tggcaggtct
     1441 gcctgaccgg tgactccgac tggttcgata atgctcctgc tgcaagtctg tataacggct
     1501 tcccacgcat cggcaccatg ctgacagcga gtcgttatca gaatcaaatt atccgctggt
     1561 tactacgtcc tttgcccggc gatatgagcg atttctggct cctgactgca cgggagttga
     1621 gcgtgcttaa gattctgatg cagggtatga gtttcagtga gattgcccgt tatgaacaac
     1681 gcagttcaaa gaccctgcat gcgattgcca cacgggcttt aatgaaactt ggactgggaa
     1741 cactgagcga tttcaggctg ctctatacgg gatgcggtga cagcagagtg aaacggaata
     1801 tacggctaca tgcaaaatat atcggacaac attccgctca tcgactcact cagtatgtgc
     1861 agaacatggt atccggaatt ctttatccag agaaagttgc cgttggtgaa tcaataaatt
     1921 ctgacgccat atccggaaaa gtactttccg gacaaagaaa aaatatgtct aaaaaaagac
     1981 caggattgtt atgacacagc agcgttgtta ctacccccca ctgcggcgca tacggactcg
     2041 ctacagtgta ggagccaaaa tatttctact gctctgggct gcaggattgg gaggcacgct
     2101 gtacagcctg tacagcgtgc tggcgtactg acatctgtga gggaaggatg cgtttcgttc
     2161 ttttctgtcc ctcaggaatt gtaccagcgc agttcgcagc tctatcaacc ggttctgtcg
     2221 gcaatgttac ctgcatcaga acagaagagg agttacgcaa taaattgcgg cacaggccac
     2281 agagtgtcgt gatatcggca gggcgacctg ccgaatgcgc tgaaatgtgg ttccgatttt
     2341 atcgggatca ctcattcgtg gtggtgttgt gcgtcgcacc cttttttctg ccaccagatg
     2401 tgagtatttc tggcgttctc aaaaacctga ggttactgaa accaggaatg tcagtagagc
     2461 gtgtcatcag tattgcaaat gcctctggtg ggttttcagg gctgaaacat gcagaaattt
     2521 tgccagttat ggacagttac tctgtcttta tgaaagaggt gaataaccgc acaaagacaa
     2581 tcgtcatgtc agaacgcttc ccggaaaagc aaaagaaggt gttgtcgttg cttcttgccg
     2641 gacacagctg ggaatacagt gcgcagtttc tcaaaacggg gataagacag atatggcttg
     2701 ccgagcagtc gctaaaaaag cgttggggaa taccggacag catgtcattg cgggaagccc
     2761 tgctgctgag cagcaataat tttcacggtg atggaaatgc attggaaaca acgaatgcca
     2821 tgacattgcg tgaaaacgga aataccaaca ggtattccgt ggtggcaaat gcagggacgc
     2881 aggctttatc tctgcataaa tataaataag tgggaaattg aaattagtta ccagaatgga
     2941 gaatttcttc atgaaaaaca gtaaggcatt ttaccgcagc gcattagcaa cagctattgt
     3001 tatggctctt tctgcaccag cattcgctgc tgataaagcg gtatcaactg attcggttac
     3061 gctgaataaa gataagacaa ctctggatca agatgttgtt attaacggtg ataacaagat
     3121 tacagccgta acaattgaaa cgtcagattc agataaagac cttaatgtta cttttggcgg
     3181 tcacgatatt accgccgcat caacggtaaa ccaaggtttc gttgaaggtg taaaagttag
     3241 cggtgacaaa aatgttgtga ttaatgctac aggctccacc atcacagctc aaggtgaagg
     3301 cacctatgtc cggtctgcaa tggtcatttc ttcaactggc gatgttgttg ttaatggcgg
     3361 taatttcgtt gcaaaaaatg aaaaaggtag tgcgacaggg atatctctgg aaggggccca
     3421 gggaaataat gtcacgctta atggcacaac cataaatgct caaggtaata agagttccag
     3481 caacgcctcc acggcaattt ttgctcaaaa gggtagcgta ttgaatggtt ttaacggtga
     3541 tgcaacagac aacattaccc ttgctggctc aaatattatt aatggtcgga ttgaaacaat
     3601 acttattgct caggagaata agggaactca tacagtcaac ctgaagatta aggatggctc
     3661 agtaattggg gcggctaata ataaacaaac aatttatgcc tcagcttcgg cacaaggaaa
     3721 aggttcagca acgcaaaatt taaatttgtc tgttgctgat tcaaccatct actctgatgt
     3781 cctggccctt tctgaaagcg agaattcagc cgctaccaca acaaatgtaa atatgaacgt
     3841 tgcccgctct tactgggaag gtaatgctta tgcttatgct tatgcttatg cttatgctgg
     3901 tgtttatgct gatgacgata aagcgagtag taatctgaat ataaatcttt ccgatggctc
     3961 agtctggaaa ggaaatgttg cgggtagtac gactactggc agcaccggta ctacagaaaa
     4021 ccagtcagca accgtaagtc tgtctgattc cacctggaaa ggtaacgtgg gtgacggtct
     4081 ggatggtggt gcgcgagtca cgctgaaaaa ctccagctgg actggtgatg tcggcaacgc
     4141 caaaactgct gtttttctga cggatggttc tgtgtggaat ggtgctgttt ccattaacgc
     4201 gccagtggac cgcgcatcag taccaatggg ctcggttcaa ccaaataaaa gcagtgtatc
     4261 tctgcaaaac gggtctgtct ggaatgttac gggagcctca actgttgatg ctctggcagt
     4321 aaaagacagt acggttaata tcacgaaggc tacagtcaat actggcacgt ttgcttctca
     4381 aaacggcact ctgattgttg atgcctcttc tgaaaacacc ctggatatca gcggtaaagc
     4441 gagcggtgac ttgcgtgttt acagtgcggg ttcattggat cttatcaatg aacaaacggc
     4501 atttatttct accggcaaag acagcactct aaaagccaca ggcacaacgg aaggtggtct
     4561 gtatcaatat gacctgacac agggagctga tggtaacttt tatttcgtaa aaaacacgca
     4621 taaagcatcc aacgccagct ccgtgattca ggcaatggca gctgctccgg ctaacgtcgc
     4681 taatctgcag gccgacacgc tctccgcccg tcaggatgct gtccgtctga gcgaaaatga
     4741 cgagggtggc gtatggattc agtactttgg cggtaaacag aaacatacca ccgcaggaaa
     4801 tgcatcctat gacctggatg taaacggtgt aatgctgggt ggtgataccc gcttcatgac
     4861 tgaagatggt agctggctgg ccggtgtggc gatgtcttct gcgaaaggtg acatgaccac
     4921 catgcagagc aaaggtgaca ctgaaggtta cagcttccac gcttacctga gccgccagta
     4981 taacaacggt atcttcattg atactgctgc acagtttggt cattacagca acacggcaga
     5041 tgttcgcctg atgaatggtg gcggtaccat caaagctgac tttaacacca atggttttgg
     5101 tgcgatggtt aaaggcggtt acacatggaa agacggtaat ggcctgttta ttcagccata
     5161 tgccaaactg tctgctctga cgctggaagg tgtggattat caactcaacg gcgtggacgt
     5221 tcattctgac agctataact ctgtgctggg tgaggccggt acgcgcgtgg gttatgactt
     5281 cgctgtgggc aactcgaccg ttaaacctta tctgaatctg gccgcactga acgaattctc
     5341 tgatggcaac aaagtccgtc tgggtgatga gtctgtcaat gccagcattg acggtgcagc
     5401 attccgcgtg ggtgcaggtg tacaggctga tatcaccaaa aacatgggag catatgcaag
     5461 ccttgactac accaaaggtg acgacattga gaatccgcta cagggtgtag ttggtatcaa
     5521 tgtgacctgg taagaataaa gcagtcggtt cccctgtcga ctgttatacc ccctgcaggc
     5581 agcagtatgc tgcctgcagg ctataaatat ccagtattca tgatgctgga tatttgctga
     5641 tatttaaccc gaacattcct gatacagact attaattcat ttatctgcgg cagattttat
     5701 tttctgaata tattatgaat gaacaaatca aacaggatat cgatctgatt gagattttat
     5761 tttatctgaa gaaaaagatt cgtgttatcc tttttattat agctatctgt atggcaatgg
     5821 tgctgttgtt tctgtatatc aataaagata atataaaagt gagttacagt ctaaaaataa
     5881 accagacaac accaggcata cttgttagct gtgatagcaa taataatttt gcctgtcaga
     5941 ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc agaccagatg
     6001 tcaagaacag agaaataaag ctggaatggt caggagataa gagagattta ccaactgctg
     6061 aagcggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca gaataccata
     6121 atggcaggca agttcttgat gagatacaaa cgccttcagc aattaacagt gagctttata
     6181 caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat ggctgtgtaa
     6241 ccataagctc accggaaata aaaagtaaat accctgctgc catttgtctg attctgggat
     6301 tttttctaag cattgtgatt tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat
     6361 accaacaaaa ctctgggcag taataaacag cattctgaca gagagtgcct gtataaaact
     6421 atagtgacaa tgaccgggaa gggagcccgt cttcaggaat ggattcctgt ctttttcact
     6481 gtaaatatcc tggcattatt aatgtcagga tatttacaga agagcagcat aaccatgacg
     6541 gaacggagtt aagtcaggtt gtcattagcg ctattcgcaa cctcgtgccg ccttcaggtt
     6601 tatgtccaga cttcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc
     6661 ggaccatgaa ccccgacgac tttgataagc tcaatgtccg ccgttgaact gggaccactg
     6721 atgatgttaa tacatgacgg cattcgttca ccggcctgcg ctttctgatg caatttttct
     6781 gcgagttgcg ctacacgcgg caggattgtg cttttacgca ggataaaaag agaagattcc
     6841 gggagcaggc tcaatgaacg cccgcgcccg gcggcggaaa aaagaaccac gcctcccgat
     6901 tcggttaaac catattcagc atatacgacg cccactttag cctgctctgc ttgtgatatg
     6961 ttctcaatac ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttcg
     7021 ctaatcccca attcctccaa cctcgtgtca ccactaatca cgaccgactg atctcccagc
     7081 tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag ttcacagcgc
     7141 gtcaacataa catcgctggc aaactggata aacgcgtcac agcgctgctg ttgggtaagt
     7201 tgggtaaacc gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt
     7261 cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa attcgctccg attatccatt
     7321 ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc
     7381 aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc
     7441 gccattgata aaccagcttg ccgcatgagc accagccatc atcccaactt tccacaatcc
     7501 tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat
     7561 ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac
     7621 cggacacacg ctgtcacaag cggtgcataa agagcaggcg tagggtaaat ctttaaaatc
     7681 tttatagccg ccaagtaacg gggaaatcac cgcaccaatt ggccctggat aaatagagcc
     7741 atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcaacg
     7801 aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac
     7861 aataaccaga tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag
     7921 ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa
     7981 tacatctacc tctgcaaacg tgggagcaat acgctccatt cccatcactg caatatgcgt
     8041 tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc
     8101 ggtctctgcc accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc
     8161 gcggattttt tgccgaatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc
     8221 cagacgttcg tgtagcaccc gacggatctg atggcgatct ttatgaattg ccgggaccac
     8281 aacatgagag ggcggatctt gatccagctg gagaatgtat tcacccagat cggtttcaat
     8341 cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga
     8401 tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt
     8461 agcgtcttct ttggtttttg caaaatagac gtgaccgccg ttttgcgtca ctttttctga
     8521 gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg
     8581 atcgcgccac tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccgat
     8641 acgctgctgc gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat
     8701 gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca
     8761 tcaacacttc agcaatatgc atcactttga ctttcagccc ttcccgttgt aatcgtccac
     8821 tgatgttcag caggcaactc acgtcagcac ctattaaata ctcagggcgg acttccatca
     8881 ggtgcgcaac cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg
     8941 tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca
     9001 catttttcag cagcgtaagt ggttcgtcct tcacccccag cttacgggcc aggctacaag
     9061 atgggtgata caccgctctc ccttgcaaac tggcacctac atcgactacc cctaatgtat
     9121 taacaataaa agaggtgaga tcctgcatac gcgcggcaac cttttcggca cgtgatgccc
     9181 attcaggttc atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag
     9241 ctggtgaaat aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg
     9301 gaatcgcttc tttgatataa ccactattga tcgcaggctg accgcagcat ccctgtttct
     9361 ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc
     9421 ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc
     9481 cattacttca tgcccattta tgcccgatgg ttcccccctt ccgggggatc aatcatcaat
     9541 aaatatggcg ttctttttta attggctagc aacataccac ccctcatttg tatgacaaat
     9601 gataatcttt catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc
     9661 acattttata gggaaatcga cgatcatttt tggcataaga aatccattta aaaaggctac
     9721 atcccttata cctattcagt gctacttatt caggggttat tgagatatac actgatggtg
     9781 tacaaacaaa atcaccaaca ataattacgt atcaagtaat agtcggcatt ttatttaatt
     9841 aaagcaattt agtttgcgtt tttatttaaa aactgagcta gagtccatca aaaaaaatat
     9901 ataaagccca aaaccaccgt tggaaataga aatagttgaa aggctcacaa ctcccggaat
     9961 gcggattatc tttaccctga tttctggcgg ttttattcag ggtgcaagct gtctcaccct
    10021 ttcccgatat tctcccggag tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga
    10081 ttcactggca tagcctactg actcagcgat caccacaaca gggagcattt cccgggaaaa
    10141 catctgggcc gctatttgta gacgcaactt tgttaataca gccagcggcg tggttccgga
    10201 aacatcacgg aaaagctggg caaaacttgc ccgggacatg tgggcgatgc tggccagcga
    10261 ttcgacggtc caggcgtgtc ctggcatttc cagcatttgc tgtattaccg caccaagacg
    10321 tggatgcaga agcaaactga gaatgttttt ctctgtatta acctgtgcaa tccactcacg
    10381 caccgcgagg gtaaagaatg tagcgcagat ctggctacac agagcatcca ctcccggcat
    10441 tgccgatctg gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt tatattccac
    10501 gctatggttc actggtgcta caaacagcgt ttccggcaaa gatgttaaaa aataacgcgc
    10561 cgaatgttgc agccgaagag tgccacagac aatacaggtc gattcattat cgacatgact
    10621 caggcgatga gctgaatttt gtggtagcag gaccacattt cccgggcgta atgtaaaaat
    10681 ctcccccgtc ggcatttcca gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac
    10741 cgacaattcc ccggcaccat gtggaagttg ccagtcactt cctaacacgc aattcttatc
    10801 gatcgttcct tgtggagcgt taagcatcaa caaacggcta agggcatcca tatcagactc
    10861 ctgagtggtt tttgattaca aacaccaaca atcttagaca ataattaatc taatatcatc
    10921 aaatggttaa tcatgatttt tgttttgtga ttctgattaa gaaaaagaaa ataccagacg
    10981 ataagacaaa atatcaagcg ttgtgaagaa atgttatttg ctcttttgcg tctatagtca
    11041 tgatgtcaaa tgaacgcgtt tcgacaggaa atcatcatga ataaatatca ggcagttatt
    11101 attggttttg gcaaggctgg aaaaacatta gccgtcacgc tggcaaaagc aggttggcgt
    11161 gtggctctca tcgaacaatc aaatgcaatg tatggcggga cctgtattaa tatcggctgt
    11221 atcccaacca aaacattggt tcatgacgca cagcagcaca cagattttgt ccgtgccata
    11281 cagcgtaaaa atgaagtggt taatttttta cgtaataaga attttcataa tcttgcggat
    11341 atacccaata tcgacgtgat cgacggccag gcggagttta tcaataatca tagcctgcgt
    11401 gttcatcggc ctgggggaaa tctggagatt catggcgaga aaatttttat taataccggt
    11461 gcacaagccg tggttccgcc aattcctgga attaccacca cgcctggtgt atatgacagc
    11521 accggattac ttaatctaaa agaattgcct gggcatttag gtattttggg cggcggatat
    11581 attggcgttg agttcgcctc tatgttcgct aattttggca gcaaagtaac cattttagag
    11641 gcagcttcgc tgtttttgcc tcgggaagat cgggatattg ctgataatat cgcgacgatt
    11701 ttacgcgatc agggtgtcga tataatcctc aatgcccatg tggagcgaat cagtcaccat
    11761 gaaaatcaag tgcaagtgca tagcgagcac gcccaactgg cggtggatgc actgttaata
    11821 gcttccggtc gtcaaccagc taccgcttcg ttacatccag aaaatgccgg tatcgcagta
    11881 aacgagcgcg gggcaattgt cgttgacaag cgattacata ccaccgcaga caatatttgg
    11941 gcgatgggag atgttaccgg cgggctgcaa tttacttaca tatcactaga tgattaccgc
    12001 attgtacgtg atgagttact gggtgaaggc aaacgtagta ctgatgatcg gaaaaatgtg
    12061 ccttattccg tatttatgac accgcccctg tccagggttg gtatgacaga agaacaagcc
    12121 agagagagtg gtgctgatat tcaggtggtg acattgcctg tagctgcaat tccgcgcgcc
    12181 agagtgatga acgatacccg tggggtatta aaagcgattg ttgataataa aacccaacgt
    12241 atattagggg catcactgct atgtgttgac tcccacgaga tgatcaatat agtgaaaatg
    12301 gtgatggatg ccgggctgcc ttatagcata ttacgcgatc agatatttac tcatccgtcg
    12361 atgagcgaat cactcaatga tctattttca ttagtcaaat aaactcaaaa tcagacgcca
    12421 gaacaaatat cctggcgtct cagagaaaag aatcttatta attctctgtt actctataaa
    12481 ttcttaatca cttcattgat ggtattttat atgtttaaaa aatctgtttt atttgcaaca
    12541 ctattatctg gcgttatggc attttccacc aatgcagatg ataaaataat tctgaaacat
    12601 atcagcgtct catcagtatc agcatcaccg acagttcttg agaatgccat tgctgatatg
    12661 gccagaaaat ataatgcttc atcctggaaa gttacatcga tgcgaattga taataattca
    12721 accgcaacag cagtattgta taaataagga tgtttacaat ggaaagatac ctgcacctgt
    12781 taagtcgggg agataaaatt ggcctgacat tgattcgtct gagtattgcg attattttta
    12841 tgtggattgg gttattaaag tttgtccctt acgaggcaga cagcattaca ccattcgtcg
    12901 caaacagtcc actaatgtcg ttcttttatg aacacccgga agactataaa cagcatctga
    12961 ctcacgaagg cgaatacaaa ccagaagcaa gagcatggca aacggctaat aatacctatg
    13021 gtttttccaa cggtcttggc gtcgtggagg tgattattgc tctgctggtt ttggctaatc
    13081 ctgtcaatcg ctggttaggt ttattgggtg ggctgatggc atttaccacg ccgctggtaa
    13141 cactctcatt tttaatcacc accccggagg catgggtacc cgcattgggt gacgctcatc
    13201 atggtttccc ttatttatcc ggtgctggtc gtctggtatt gaaagatact ctgatgctgg
    13261 caggtgctgt catgataatg gcagattcgg cgcgggatat tcttaaacaa cgcagtaatg
    13321 aatccagttc aacgttaaaa actgaatatt gataatccga acactgtctg ttgttcactt
    13381 tttcaggcgt caggccccct ttctgatgat gaagcctgac gccatattac cggagatatg
    13441 acagagtgaa gatcaatatc actcccctgt taaaagattc ttgtcgtctc aggtaaaata
    13501 agtgattaat atcaatcatg tatttttaca gcaagacaac cacgaacaaa ctcaggatta
    13561 aaatgtttta ccgattcccc aacataaccc aaatcaagcg aactaatttg cgccatttct
    13621 ttctctgtca gtgagaaatc ccagatagca aaattttctt cgatacgctg ttgatgtgtc
    13681 gatttcggaa taacgaccac tccccgttga atattccaac gcagaatgac ctgagaaatc
    13741 gatttttgat gggcatctgc aatactctga agcagattat tttcaaaggg tttatgtcgt
    13801 ccaccaccta acggagccca tgcttcaggc tgcacgttat aatacttcat ggttgccagc
    13861 gcctctggtt gggcaaaata gggatgcaac tcgacctggt tgaccatcgg tttaactctt
    13921 accgtttcac aaaagttcgc cagaacatga gggtagaaat tggaaacccc aatcgctttt
    13981 aatttgccct cttcataggc atcttcgagt gcacgccacg cgctaaaata atcgcgcatg
    14041 gcctggtgca ataaatagag atcgaagtat tctagtcccg atttttttaa tgatgcttca
    14101 atacctgctg ctgcaatatc ctgattcaac atatcctgca cccacaattt agacgttata
    14161 aataactctt ctcgggtaca taaaccttca gatatagctt cacgaacggc ttcaccgacg
    14221 gcatcttcat taccgtatac cgctgcagtg tcgatgagtc gataacccgt gcggatagcg
    14281 ttcaacactg actgtttgca cacgttttta tcggtaacct gaaaaacacc aaatcccatc
    14341 atcggcattt tcagattgtt acttaatacg gaaaattcca ctgtcagtcc ctccattagg
    14401 catgaacaat gagtctacgt taaaacgtaa cctcaaatta gtatgtggat tttgatatca
    14461 cttatgcaaa aaattcatta ataatgtagg actgaaacct ctctattttc ggggacaacg
    14521 aagcagacgc taccagtgct tttgccttcg cccttgctgt ttttgataca cttagggccc
    14581 agggcataac gaaaatgtgc gatatgacaa agaattaacg gagaatgtga tgatcaggca
    14641 gaagattcta cagcagctcc tggagtggat tgagtgcaat ctcgaacacc ctatttcaat
    14701 cgaagatatc gcacagaaat ctggctacag cagacgcaac atccagcttc tgttccgaaa
    14761 tttcatgcat gtgcctttgg gagaatacat tcgcaaacga aggctttgtc gtgccgccat
    14821 tcttgtccgg ctctccgcga aatctatgct tgatattgca ctctctttgc attttgactc
    14881 acagcagtca ttcagccgcg aatttaaaaa gttattcggc tgctctcccc gtgaataccg
    14941 ccaccgtgat tattgggatc tcgcaaatat cttcccttct tttttaatac gtcaacagca
    15001 aaagacggag tgtagattag tcaactttcc tgagacacct atttttggta attcatttaa
    15061 atatgatatt gaagtgtcga ataaatcacc ggatgaagaa gtcaaactgc gacgtcatca
    15121 tttagccaga tgtatgaaga gttttaagac ggatatctat ttcgtctcca cgttcgaacc
    15181 atcaacaaaa tcggtcgatt tgctcacagt tgaaactttt gccggtacgg tatgcaaaca
    15241 aaccgattca gagatgtcga aagagtggac aattaaccga ggactgtatg cctctttccg
    15301 ctatgaaggt gagtgggagc actaccctga atgggcgcga aacctctatc tgatggaatt
    15361 acctgccagg gggttagcca gaatgaacgg cagtgatatt gagcgatttt attacaatga
    15421 aaacttcgtc gaaattgata gcaataacat tgtttgcgaa atttttatcc ccgttcgccc
    15481 ggtttagcct gtaactccat cacataagat ttaccctacc ctgccaccgt ctaatcggtg
    15541 ccagggtagg tattcagtaa ataatggcgc tgcgcatgtc aacgccatta ttatttatta
    15601 catcgtcgtt tttatttctc ctcagcgcct tcagtatctg caggaacggc gttaattgtc
    15661 acagtcactg gtttcatttc ataccctcca tatgtcaaag agttgaatgt gaatgtgtat
    15721 gtacctggct tgttggtact aaatgtacgc gtgattttcc cgctcgcgaa atggtcattt
    15781 ttcgacggca ggaactgata atccgcctct gtcacccctt ccggagcctc aatgtcgacc
    15841 cacatggttc ctcctacggg gttgacaccc gacatcagag taaccgtcag tgttgcagta
    15901 gtcttcccgt ccgccaccag agaatctaca cgctctggag atgcagcgag caccgcgttt
    15961 ttatcatccg cgacgaattt agattcggcg gttttcgttg atacctgacc gttgttctga
    16021 ctcacttgcg ccgtgagtgt atatgtcgcc gcaacagttg tggtggcggt gaaaatagcc
    16081 tgcccttgct cattcgttgt cgccgtccca ttgggatcca gaactaactt cgccgggctg
    16141 gcagtcagcg tcacttcgct atctttcagc aggttgttat tggcatcagt caccgtaact
    16201 ttatacgtct gtttggtttt gccgtccgcc actgcacggt tgccaatcac ctcaatactg
    16261 gagatggtcg ccgtagtctt atcagccaca aacgttacag actgcgcatt cgaaggctgg
    16321 tcatttcctg ctgacgccgt gatctcatag gttccggcag tcacggaaac cactttcagt
    16381 tccgctttac cctccttgtc ggcgttcacc atgatattac cgtctgcaag cggtttgacg
    16441 ccccgaggca gattaaagac gaccacagcg ccaggaagaa ggttgccgta ttgatcctta
    16501 accgttgccg tcagcgtaaa ggcatctttg ccgtttgcca ctttttgagc agcggcgtct
    16561 acctccaggc tcgcctgacc ggtactgaaa tccgccttga atttcacctt gaccgtcttc
    16621 tgagagtttt tcaccaaggc ctcgatctca agttcccctg caaccgttga cataagctca
    16681 atgtccactt tacctgccgc attggttgtt actgtattcc ccgtcttgct agtcacaccc
    16741 tgcggtaaag tcagcgtaac ttcttgcccc tgcaacgggt taccatagct gtccacgacg
    16801 gtcagggtta tctggttagc agactgtcca ttagccagtt ggttattaac cttcactgtc
    16861 atatcacgaa tctcagcctt agatgcgtca cctgcaacat tcagcaccag tggctgagca
    16921 acggcatttt ggccgttcac tcgcggcatc acagacaact gacccgcggc agatcccagc
    16981 gttaaggtcg acacgtagac cccattacct ttctctgtcc agtttcctgc tgaaggacgc
    17041 tcactccccg tgctggcggc accactaaac actggtgcat ctggcttcag cccggtgacc
    17101 ggattcccgt acgcatcctt cacggtgaag gtgagttccg tcgtcgtttt aaccgttgga
    17161 gttttattgt ccgcaacaag cgtagagttc gctgatgaca tctctccggc aataacagtc
    17221 agctgcgcgg cttcggtggc tgcaggctgg ccgttgaaga gcggcatgac acgaagctcg
    17281 cccgtctttc cgcctgtagt taacgtagca acataggaac cgtcaccttt ttcggtccaa
    17341 ctggaaacgg tcgccccttc agaggcggtc cccgtcaaac ttgccgacaa cgaaagacca
    17401 ctgatagcgt tgccatgcgc atctttcgcc accagcgtta cggttgtgct ttcaccggct
    17461 tttacgtgat cttctgcgac agagactttc gactggtttg aagataatgc atcagccacc
    17521 acggttactt ttgccgcatt tgctgccgcg tcctgcccat tgagcttcgg cataacgtct
    17581 aattcacccg ccgtagtgcc gagagaaatc tgcgcagtcc aggtcccgtc gccattatcc
    17641 gtccagcctg atgccgtaga accagcagca gctgcgcccg ataatgacgg tgcatccgga
    17701 ttgaggccag ttacagggtt gtcattagca tcttttgccg tccagattgc cgtaactgta
    17761 ccgccaacca ccggtttatc aggattcagg gtgatggacg aatgtgctgc atcaagcggc
    17821 ccggcaacaa acttcagcgt ttgttgtaat gtgcccagct gatactgctc gctgaaggca
    17881 cgcacaacaa cgtttccggc acgggtcgaa gaaaccgtgg cgctgtaaac ccctggtttt
    17941 atttccgaaa tggcaccaac ggttatacca ttagtgtctt gcggaacaag tcgcaggcgg
    18001 ctggcttctc ccgtcaccgg attaccctcg gagtccaccg ctgtcagtgt cagcgtgtag
    18061 gcttgctgac catcagcaac cacatcacct gacggctcgt tagcgctcag ggtggagttt
    18121 gccacatcca tcatcgtggc ccgcagttct gcagtgacag ttttgctcat gtcatcaacg
    18181 ctaacagtaa tcgttgcctc acctgactgc gttccggtag taaagacaga ctgatacacc
    18241 cctgcttcag tttcggtgaa ctcacccagt gttggctgtg cctgtgattt agtggccttc
    18301 agggtacgag tcacaatatt tccagccggt ttgaaggtta gttcagtctt gatctgatct
    18361 ttcatgcccg tgactggctg accctcggcg tcgcgcagag acagcaccaa cggcttttgc
    18421 tcattaccgt tagcaagcat ttgaatacgg ctctgaccgt caagcgttaa cgccgtacga
    18481 tcggcgctca taccagctcc gctaataacc acttctgtct gcacacgttt cgaggcattg
    18541 cctttgttat cgtaggcaat cgctgaaatc gcataataat tgtctttgcc tgcctgataa
    18601 gccgggagcg tcacttgcca ctgattgccc tgccccgtaa tttttccgcc tgcggccagc
    18661 aaagacggcg cttcccattg cacatttttc agaccgtgag ttgctttgct gaccacaagc
    18721 cccagggaaa ccgtctggcc gcccttgcct tcaatacgct caggcagagc aatacggatc
    18781 acttcagatt tgcgatactc aagaacgata ttgttattac gctcaaccag gtcatagcgg
    18841 ctgcctgcca gcattcgacg ctcgcgaatg ctgtctgtat cgagttgttt ttccagaggt
    18901 tcgccaatcc gataattaac ttccaggcca aagcgagtgt cattctcacc actcttgccc
    18961 tgcttatgcc cggcactcag ggtcagaaga ggcactggcg tgtaattcac ttcagcggta
    19021 atcgcatgtg gatctttctg gcgtttatct ttaccaaaca gcccgacttc atcgccataa
    19081 tactgttcat acatcaggct tgcgccaagc tgcggccagg caggtaaata gccctcagca
    19141 cgaatatccc agccattcgc cgggcgttcc tgataatcct caacatccgg cgattttttc
    19201 cagccagaag cccggatata accattggcg ctcagtttca aataatcacg ccagtattcc
    19261 gcaccaacac caatgcgggt atgactacgg gataaatcat gatcgataaa agtattcacc
    19321 cccgccatcc agtcattttc tgaaaaatga cgccagccaa aaccaatatt tgactgagta
    19381 cgatcgtcgg tacgatgtat tgccccctga gtgaacaaca tatttgtcgg tgtatcataa
    19441 atcggataaa gcatttccag cgaagagtcc ttcagcgaga aatttttatc gacattcagt
    19501 ttgacgcgcg cagtaccgta tttcccgagc cactcctgaa tttcctggtt agctttagcg
    19561 gtggccattc cggtaataaa gttacgtgtc gcatcgctat ctggctgact gcttaaaaat
    19621 gtcccggcat tagcggcaag cgacgcgaca tttttctcca cgttattatc agcagttacc
    19681 gtagtatttt ccatgctcaa ccgtggctga accgcatgct gcgcacgtgc tgccattact
    19741 ggcgtaaagg tgactgcgag tggaaaaaga acctgaacag agatatttgc ccacgccacg
    19801 cagcgggcca gaactgaata acgaaatcgt ggctgtttat tgtctgtttt ataacgtgac
    19861 attgagataa tttcctgatg aacgaattaa cgaacatagg cagacacaaa taatggcgtc
    19921 tgcttgtcag aaaaaccggg gatttcccgg tttccttttc cacagaaaag gaaatacctt
    19981 tatgtaagaa agtacaccgc cacctaatat atccagtgtc tgtagccact ca
//
LOCUS       DAZGTJ010000088        19341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000088 DAZGTJ010000000
VERSION     DAZGTJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.088"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..1028
                     /locus_tag="ACHK8E_003914"
     CDS             126..1028
                     /locus_tag="ACHK8E_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0346120.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            1175..2395
                     /locus_tag="ACHK8E_003915"
     CDS             1175..2395
                     /locus_tag="ACHK8E_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0346121.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2368..2997
                     /locus_tag="ACHK8E_003916"
     CDS             2368..2997
                     /locus_tag="ACHK8E_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0346122.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2998..4158)
                     /gene="ybdL"
                     /locus_tag="ACHK8E_003917"
     CDS             complement(2998..4158)
                     /gene="ybdL"
                     /locus_tag="ACHK8E_003917"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP0346123.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4267..5355
                     /gene="hcxA"
                     /locus_tag="ACHK8E_003918"
     CDS             4267..5355
                     /gene="hcxA"
                     /locus_tag="ACHK8E_003918"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP0346124.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(5365..5562)
                     /locus_tag="ACHK8E_003919"
     CDS             complement(5365..5562)
                     /locus_tag="ACHK8E_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0346125.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5675..7780)
                     /gene="cstA"
                     /locus_tag="ACHK8E_003920"
     CDS             complement(5675..7780)
                     /gene="cstA"
                     /locus_tag="ACHK8E_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP0346126.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7961..8374)
                     /gene="entH"
                     /locus_tag="ACHK8E_003921"
     CDS             complement(7961..8374)
                     /gene="entH"
                     /locus_tag="ACHK8E_003921"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP0346127.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8377..9123)
                     /gene="entA"
                     /locus_tag="ACHK8E_003922"
     CDS             complement(8377..9123)
                     /gene="entA"
                     /locus_tag="ACHK8E_003922"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP0346128.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9123..9980)
                     /gene="entB"
                     /locus_tag="ACHK8E_003923"
     CDS             complement(9123..9980)
                     /gene="entB"
                     /locus_tag="ACHK8E_003923"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP0346129.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9994..11604)
                     /gene="entE"
                     /locus_tag="ACHK8E_003924"
     CDS             complement(9994..11604)
                     /gene="entE"
                     /locus_tag="ACHK8E_003924"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP0346130.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11614..12789)
                     /gene="entC"
                     /locus_tag="ACHK8E_003925"
     CDS             complement(11614..12789)
                     /gene="entC"
                     /locus_tag="ACHK8E_003925"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP0346131.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12978..13934
                     /gene="fepB"
                     /locus_tag="ACHK8E_003926"
     CDS             12978..13934
                     /gene="fepB"
                     /locus_tag="ACHK8E_003926"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0346132.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13938..15188)
                     /gene="entS"
                     /locus_tag="ACHK8E_003927"
     CDS             complement(13938..15188)
                     /gene="entS"
                     /locus_tag="ACHK8E_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP0346133.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15299..16303
                     /gene="fepD"
                     /locus_tag="ACHK8E_003928"
     CDS             15299..16303
                     /gene="fepD"
                     /locus_tag="ACHK8E_003928"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP0346134.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16300..17292
                     /gene="fepG"
                     /locus_tag="ACHK8E_003929"
     CDS             16300..17292
                     /gene="fepG"
                     /locus_tag="ACHK8E_003929"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP0346135.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17289..18104
                     /gene="fepC"
                     /locus_tag="ACHK8E_003930"
     CDS             17289..18104
                     /gene="fepC"
                     /locus_tag="ACHK8E_003930"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0346136.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(18101..19234)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK8E_003931"
     CDS             complement(18101..19234)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK8E_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP0346137.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
BASE COUNT         4678 a         5376 c         4865 g         4422 t
ORIGIN      
        1 gacagctatg ctattttaga aaatcttaat ccaaagttaa gcagaaaaac ataatacata
       61 taaatacaaa aacattattg atcaagtgaa tgatttatgg aagagacaat acaacggagt
      121 atgttatggc taatctttat gatctaaaaa aatttgacct caatttactg gtcatatttg
      181 aatgtattta tcaacatctc agcattagta aagcggcaga atcattatat ataacgccct
      241 ctgctgttag tcagtcgtta caacgtttac gtgcgcaatt taatgacccg ttattcattc
      301 gctcagggaa aggtatcgcg ccgactacaa cagggttaaa cctgcatcac catcttgaga
      361 aaaatctcag aggccttgaa caaacgatca atatcgttaa taagtctgaa ctaaagaaaa
      421 attttattat ctacggtcct cagcttattt cttgtactaa taacaacatg ctaatccgct
      481 gtcttagaca agatacttca atcgatatag aatgccatga catcctgctt tcagctgaaa
      541 atgccgaaga actgttggtt caacgcaaaa ctgacctggc aattacactg caaccagtaa
      601 ttagtcgctc tgtcatctgc atgccattac acactattcg caattccctt atatgtagca
      661 acaaacatcc tcgtattact gataccagta actatgaaca aattatcgcc gaagaattca
      721 ccctgttgat atctaaatca gcagggattg acgaaattca aatggacata gatgaacgtt
      781 ttataaaccg taaagtttca tttcgcagtt cttctttgtt gactataatt aatagcattt
      841 ctgttactga cttattaggc attgtgccca ccgaactgta cgatttacat cgtgattttt
      901 tgaagttaaa agaaataaaa ctagaacaac ctcttcctgc ggttaaactt tatatttcct
      961 ataacaaggc ttcgttgaac aaccttgtct tctcccgatt catagatcgt ttaaatgata
     1021 gcttctgatt cattaccact gacaaataat atattacgca tcaattaatt cattgtgtga
     1081 atataaaata atgtgctcta tcttgcttcg tttatcctat aattagcgga ttcatctcac
     1141 gagtcacccc gtaacgttca gagagcgttt ttagatgtct atttataaaa tcccgcttcc
     1201 actgaatatt ctggaagctg caaaagaacg tattacctgg acacttaata ccctaccccg
     1261 tatctgtgtt tctttttcgg gtggtaaaga ttccggatta atgctgcatc tgacagccga
     1321 aattgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aagcgcagtt
     1381 ctcatgcact attaactatg ttcagtcatt acgtgagttt tacgccgatg tcatcgaaga
     1441 gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttctcaat accaacccga
     1501 atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac
     1561 cgatcctgac tttttctcct tttaccagcc tggcatgacc ttcgaacaat ttgtgcgtga
     1621 gtttgctgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca tccgtgcaga
     1681 tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgatga
     1741 tatgccctgg accaccgccg caccaggcgg tcatagctgg tacatttacc ccatctacga
     1801 ctggaaagtg gctgatatct ggacctggta tgctaaccat caacagctct gtaacccact
     1861 gtataatata atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt
     1921 tggcccggag caacggcaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc
     1981 tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa
     2041 tcatttttat ggtcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc
     2101 attattgctg ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc
     2161 catttatttg cactggtatc agaaaaaagg catcgaagtt ccacaaaccc agcaagggga
     2221 cattggcgcg aaagatatcc cctcctggcg gcgaatatgc aaagttttac tcaataacga
     2281 ttactggtgc cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa
     2341 cgaacggata aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag
     2401 gatttaaccc aatttctcgc cagcttgccg gaagatgatc gcatcaaagc gattaatgaa
     2461 atccgcatgg cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg
     2521 tgggttaaaa acagccaact tatacctaat gattacaacc caaacaacgt ggcaccaccc
     2581 gaaaaaaaac tgctgaaaaa atccattgaa acagatggtt ttacccaacc cattgtggtc
     2641 acgcatacgg ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca tgaaattggt
     2701 aaaggctcat catcgttaaa attacgctta aaaggttatt tgccggtgac ctgtctggaa
     2761 ggtacgcgta atcaacgcat cgccgcgact attcgtcata accgcgctcg tggtcgccac
     2821 caaatcactg ccatgtccga aattgttcgt gaactcagcc agttgggatg ggacgataat
     2881 aaaattggca aagagctggg aatggacagc gacgaagtgc tgcgcctgaa acaaattaac
     2941 ggcctgcaag agttgtttgc cgaccgtcaa tattcccgtg cctggactgt caaataacta
     3001 cagctggcgc aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag
     3061 acgaatcagt ttatggggga agggatcggc gcaaaatacc gacagcggga tcgccgctac
     3121 accgtgctcc cgcgtcagcc actggcaaaa cttaacgtca tctagcgaag aaaccgcgct
     3181 gtaatccacc agcaaaaagt atgtaccttc gcacggtaaa atctccagcc gactctcact
     3241 taaggcattc accagaatat cgcgtttctg gcgataaaag tccggtaacg caagataatg
     3301 ctcaggttct gcacgtagca tatcggcaag cgccaactgt gccggggtat tcaccgaaaa
     3361 ggtcagatac tgatgcactt tgcggatctc ggcgctgatg ggcgcaggcg caacgcaata
     3421 acccactttc cagccggtca tatgataggt cttgccaaac gatgacaccg ccaccgctcg
     3481 ctcacgcaat tgcggatgcg ccagcacgct ggcatggccc tgttgtgaaa aattgatgtg
     3541 ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc
     3601 ggcgaaatca gcctgctgcc agacagttgc actggggtta tgcggggtgt tgaggatcac
     3661 cagtcgggtg cgctcgctta acaatgcggc aaattcctgc cagtccacgc gaaaatgcgg
     3721 tggttgcagt gcaatacgtt tcactattcc ccccgaaagc gcgatggcgg gcgcgtaact
     3781 gtcatagctg ggatcaaagc agatcacttc atcaccattg cgcaccaatg cggtaatcgc
     3841 tgcatataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catccggttg
     3901 atagccatac agacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt
     3961 catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata
     4021 gcgcggacca tcaaaatcag gaaagccttg tgaaaggtta atcgcctggt gttgctgcgc
     4081 cagcgcgctc atccgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat
     4141 cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa
     4201 cacgatgcta gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa
     4261 cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat
     4321 cccggaagtt tcaatcacct gcacgatttt ttcactgatg aacaactttc tcgcgcggtg
     4381 tggatctacg gcgaacgcgc cattgctgcg gcacaaacca aactgccgcc tgcgtttgaa
     4441 ctgccagggg taaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtgcaacaa
     4501 ctggcggcgg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcacta
     4561 ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc cgtttgttgc cgttccgacg
     4621 atcgccgcca cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag
     4681 gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga gccagagatt
     4741 atcctcaatg cgccgcaaga atatctgctg gcggggatcg gtgacacgct ggcgaaatgg
     4801 tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcggctgggg
     4861 atcaataatg cgcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcgctgagc
     4921 gatcagcaaa aacaacagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct
     4981 ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggcccatgcc
     5041 gtgcataacg gtctgaccgt tttgccgcaa accgagaagt ttctccacgg caccaaagtc
     5101 gcctacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta
     5161 acagaggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc
     5221 aataatcagg tggagatcga caaaatgatt gcccacaccc tacgtccggt ggaatccatt
     5281 cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaacgtggaa
     5341 tcgtttaaag cctgacgtac aaatttagca gcagcgcgcg ccgccttttc caccgtagcg
     5401 cgcgtcctgc cgctcccgga aaaactcctc gtaggtcatc ggtgtttgat cgggatggtt
     5461 aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca gtttcgccgc
     5521 ctgacctaaa tattttccgg cttttgccag tgaatcaaac atctaaacct ccggtaatac
     5581 aaatgccctc ctgaattaag agggcttaat tgctatcaac acgctcggga tgagagtaag
     5641 gggaacgctc agcacattcc ccctcactct gactttagtg cgcgcctttc gcctgcgcca
     5701 cgatctcctc gacattttcc ggcatcggct catatggcgt ttctttcgcc gttggcttcg
     5761 gctctttcaa tgccaccagt gccgtcttaa tcgagaacag cgccagaacc accacaacca
     5821 ccataaagaa gatggttaat ccggcatcca gacggttgtt gaacaccagt tgcgccagtt
     5881 gtgactcagt atactgcgac ggaatattgc cgctgtcgat cattgcctgg aacttgttag
     5941 caatcgccag gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca
     6001 gggtacaaat cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt
     6061 tcattttgaa cagcaccaca gcacagagca tcagcgccat ccctgccagc atctggttgg
     6121 caataccaaa cagcggccac aaggtgttaa taccgcccag cggatcgacc acgccctgat
     6181 gcaggaagta gccccacgcc agcacgcaca gcgccgttgc cagcaggtta gcaggcagtg
     6241 aatcggtccg tttcaggccc ggagacacca cgcccagcag atcctgcaac ataaagcgcg
     6301 cagcacgcgt acccgcatcc accgccgtca gaataaacag tgcttcaaac agaatggcga
     6361 agtgatacca gaacgccaca tccatcatgc cgcccagcgc accgtgcaga atgtaggcca
     6421 tccccaccgc cagggtcggc gcaccgcccg cacgagaaat gatcgactgt tcacccactt
     6481 cgctggcaat ctggtttagc gtatccgggg taatcgcaaa gccccagcta ctcaccacct
     6541 gcgcggcaga agcaaccaca tccgccgtcc ctgccggagc cagcaccgcc atcgggctgt
     6601 tcatggcgaa gtacacaccc ggatcgatga tacaggcgga aaccagcgcc ataatcgcca
     6661 cgaaggattc catcaacatc ccaccgtagc cgataaagca cgcctgccct tcgttcgcca
     6721 gcatcttcgg tgtagtgccg gaagagatca gtgcatggaa gccagacacc gcgccacagg
     6781 cgatggtgat aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccatcga
     6841 caaatttagt tagcgcaggc atggtcagcg tcgggcgcat aatcaaaatg cccaccgcca
     6901 gaccgacgat agtcccgatt ttcaggaagg ttgagaggta gtcacgcgga gccagcagca
     6961 accacaccgg cagcaccgcc gccacaaaac cgtaacccac cagcatccag gtcagctgca
     7021 cgccggtaaa gtcaaagtac ggtgcccacg tcgggctttc tgccacccag ccgccagaga
     7081 taatggcgaa aataaggaac accagaccaa tcaccgacac ttcgccaata cgccccggac
     7141 gcaaatagcg caggtagatc cccataaaca gcgccagcgg aatggtgaac gcaacggtat
     7201 acgttcccca cgggctatga gtcagggctt tcaccacgat catcgccagc acggcaagga
     7261 taatgaccat gatcataaag caggccacca gcgcaatcac cccggcggtt ggccccatct
     7321 cttctttgac caactcaccc agcgagcgac catcgcggcg cgtagaaaca aacagcacca
     7381 tgaaatcctg caccgcaccg gcgagaacca ccccagcgag cagccagatc atccctggca
     7441 ggtaacccat ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg
     7501 caaaatggtg accgaacagc acttttttgt ccgtcggcac atagtccagc ccgtcgttat
     7561 ggcgcaccgc tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca
     7621 gcccataaaa acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg
     7681 cgttgatctg ttccccacga tttaaagcaa tgtatcccag agcaaatgct cccattacag
     7741 agagcactgt ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg
     7801 tccatagaga tgttacattt tgtttctata acatctttgt acaatttaac aacacggcaa
     7861 ccatgtaaat cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt
     7921 taaccgagtt ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa actgccgtac
     7981 ccagccgaca agtgcagcaa cgccgcccct gttcatcgaa aacaacaatt tcccagctct
     8041 gattttgccg accaagatgc agcggctggc agacgccgcg tacctttccc tcagacaccg
     8101 ggcgatgatg tgtggcgtta agttccgtgc ccaccacaca ctgtccgtcg cgagtcatca
     8161 taaatccggc catcgatccc agcgtttccg ccagcgccgc cgacgcgccg ccatgtagta
     8221 aaccgaacgg ctgatgagta cgggtatcaa ccggcatttc ggcttccagc acatcatcgc
     8281 ccaggcgggt atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca
     8341 gctcgtcgag cgttaaatgg cgtttccaga tcatgcttat gcccccagcg tagagccgcc
     8401 atctaccaca atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat
     8461 cgtgttggcg atctcttgtg gacgggcgat tttccccagc ggaatgccaa gtttaaactg
     8521 ctcgccaaag ccgcgaatac gctgctcttc ggcgtcatcg ctcacccaca gcgtgcgttg
     8581 catatcggtg tcggtggagc ctggcgaaac cacattacag cgcacgccgc tacccgccag
     8641 ttccagccca acgctcaacg ccaggctttt cagcgccgct ttcgacgcgc cataagcact
     8701 cataccaatg cgcggcgtgt gtgcggcgtc ggacgcaaca gtaacaatcg ccccgccccg
     8761 ctgacggcga aactggttca tggtctgctg gaacaggtta aacgcaccgc cgacattaac
     8821 cgcaaaagtc tgctgccagt cctccttact gagctgatcg gtcgcgccca tgcgcaaaat
     8881 tcccgccgca ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac
     8941 ctgcgccacc tgcgcagcgt cggcaacatc catcacttcg gtcgcaaagg gatattgctc
     9001 ctgagtgaac gcttgatcaa aacctgtaac tttcgctccc gcctcaacaa acgccagcgc
     9061 cgtggcgtag ccgatacctt tccccgcgcc agttacccag acatttttac cgctgaaatc
     9121 cattatttca cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc
     9181 agcatgacaa agtcgatatc gccatgcact ttgcgccagc gcgccgccag cgccatcatc
     9241 cgcaccgaat ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg
     9301 tccagcaacg gcaggatcac ctcacgcagc gccgctttgc tggcggggat aggtgctggc
     9361 agtaattctt cggtcatcac cactcggcca gaacgtccgg ccacatattt cagcgacatc
     9421 aaatgctcgt cacggctgaa atcggccagc gcatctgcca ccataaacgg tttaatatcg
     9481 cgcataaatg cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata
     9541 atcagctggt tacgtccact ctctttcagc atttgctcca gcggggaacg atgaaacgcg
     9601 ctgtagcgcc acttcaccag caccgtgtcg tcggcatctg gcgtcaggct atccaccacc
     9661 ttttgctgtt ccggcgaacg ggtcaggcca ggcccccaca tatcattcaa cagcgcccga
     9721 tcttcatcgc tctgctcttt cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg
     9781 cagtagtcgc gtagtgccgc aatattcgcg atcacctgct ccatcatcgg gcagttctcg
     9841 ccccagaagc tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt
     9901 tcaaaggccc agtcgacttt attctgcgga atatcgtgag actccggcag tgcgtaagcc
     9961 tgtaattttg gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca
    10021 ctgacgtaat tgttttttat cgactttccc gaccgccgtc agcggaagtg aatctacaca
    10081 ctccacgcga tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac
    10141 ctgcaccgcg cgcagcggct cttttaccac cagataagca cagctttttt cgcccatcag
    10201 ctcatcttcc atgctcacca gtgcggcgta gatcaccgcc ggatggcgca gcagcaggtt
    10261 ttcgatctct tcggcagcga tcttctcgcc gccacggttg atctgatctt tctcgcgccc
    10321 ctgcacagtg atgtaaccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc
    10381 gttggcatca aaggcgctgg cattgtgctg cgggcttttg taatagccac ggaaggtata
    10441 cggaccgcgg gtcatcaggc gtccaacttc cccttgcggc agtggatttc cttcggcatc
    10501 ggcaacccac acttcatcgt ccggacacat tgggtaaccc tgggtatgga taattttctc
    10561 cgcgctatca tcaagtcggg tgtagttcac tagtccttcc gccatgccga atacctgctg
    10621 taactggcag ccaatctctg cgggaatacg cgccgcaagc gtggcagaaa gacgtgcacc
    10681 accgacctgt aacagtttca gcgaggcaag ctgcgcccgg ctttcgcctt cagccagtgc
    10741 ctgcaaccac aggctgactg cgggcggcac cagcgcggta acgttgacct gatgtttttc
    10801 aatcaatggg aagcaaagcg tggcgctggg gtcggcagcc agaacgacag tgccgccagc
    10861 gagaaatacc cccaacgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc
    10921 gcacaggtag cgcgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta
    10981 gtagtagtcg ttatgagtgc gcggtatcag tttcggagtg ccggtggtgc cgccggaaag
    11041 ctggaaatag gccacttcat cagcaggaga tggcgtggca gtaaaatcct cagccggatg
    11101 gttaatcgca tcctgcaagt tatgctcacc gctgtcgttg agcagctgca ccacgcgaat
    11161 ggaagaatgc tctgcaacaa atgtgttgag gaaatcatcc ccgctaaata gcgcatgttg
    11221 acgatcggca atcagtaacg ccggttcaat ctggctggca taggcgttca gttcactacg
    11281 ctgatggctg aacagcgcca gcaccggcgc aacgcccagc ttcagcagcg cgaaaaaggt
    11341 aatatacaat tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc
    11401 ctgacggcgc aaactacagg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa
    11461 ctgccgctcg ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc
    11521 ggtcagtggc aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg
    11581 ccagcgggtg aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat
    11641 ggtagaaagt ttgacgcctg tttcgcgcca ctcacccaac ggtgaggacg caggcacaat
    11701 ccccgctccg gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac
    11761 cacccattca ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg
    11821 gtcgaacggc tccagttcag caataacctg agtcgcggcc tgatgcggga aaccgctcag
    11881 cgcgggggtc ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt
    11941 accttcaaag ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga
    12001 gggaacgtgt aactcactac tgcgtttgcg cagtacctct ttcatcgcct gagtcaccag
    12061 ttcatgttca tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag
    12121 cacttcatcc ggctgacgac gcgcggaacc ggctaacgga atggagctaa aacgctcgcc
    12181 gtctttacgt agcagcagtt ccgggctggc ccccagcagg acgccgccat cagccagcgg
    12241 gacatggaag ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact
    12301 atcaatggcg gcgtcagtgg tgatatcaat caaccgtgac aacaccactt tgtcgacctg
    12361 cggcgtggcg gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgttccgg
    12421 aattgcctgg cgttccacca cattcagcga ctggctgcgg gtgaaacggc gtgcggaagc
    12481 ttgtttttcc tgacgggaga acgactgcca ggattcggga atatacagcg atgaaggctg
    12541 acgtggatcg aagggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc
    12601 accggcaaac agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac
    12661 agccggttca tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat
    12721 aaagaaaaag cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga
    12781 cgtatccata tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt
    12841 ttcggtcgct aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg
    12901 caactcgggg ttgcatctgg ttgtggttac aatgaaaatg agaagcatta acttcattaa
    12961 taacaggaag ctgatttgtg agactcgtcc cgctctaccg caacgccctt ctattaacag
    13021 gacttttgct ttcaggaata gccgtagttc aggccgccga ctggccgcgt cagattactg
    13081 acagccgggg cactcatacc ctggaaagcc agccgcagcg tattgtttcc accagcgtca
    13141 ccctgactgg ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc
    13201 cgaataaccg cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag
    13261 aacgcaaact gcaacggctc tatatcggcg agccgagtgc agaaaccgtt gccgcgcaaa
    13321 tgccggatct gattttaatt agcgcaaccg gcggtgattc agcgctggcg ctgtacgatc
    13381 agctttccac catcgcccca acattaatca tcaattacga cgacaaaagc tggcagtcgc
    13441 tgttaacgca gcttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcac
    13501 agtttgataa gcaactggcg gcggcgaaag agcaaatcaa attaccgccg cagccggtca
    13561 ctgccattgt ctataccgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac
    13621 aagggcagat gctggaacaa ctcggcttta cgttggcgaa attgcccgca gggttaaacg
    13681 ccagccaaag ccagggcaaa cgccatgaca tcattcagct tggtggggaa aatctggctt
    13741 cagggttaaa tggcgagtca ctattcctgt tcgccggtga tcagaaagat gccgatgcga
    13801 tttatgctaa tccgctgctc gcgcacctgc ctgcagtaca aaacaagcag gtttatgcgc
    13861 tgggaaccga gacgttccgt ctggattact acagcgccat gcaagtgctg gaacggctta
    13921 aggcgctgtt ttaagcatta actgtcggac gctgtcacct gcggcggcgt ctggcgaaaa
    13981 cgccgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    14041 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccgcccag cagcgccgcg
    14101 cctatggcat cgcccgtcac gttttgcgcc gtccacaaac cgttaatccg ccctaacatc
    14161 gcttccgggg tttgcgtttg cagcattgtg tattgcagca acgaactaac cgcgctcagc
    14221 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    14281 ccaatggcga ggaacgatcc cagcgtggaa agcagcatca ataaccctgg tcgcgcgcta
    14341 tgtgccagct tcccgctggt taacgcgcca atagccgcac cgagcgggat cgccgcatag
    14401 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacaggacc
    14461 cgcaccgcgc tcgccatcgt caataaacca cccaacagcg caatccctcc taccagcggg
    14521 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc acgcggctgc
    14581 ggtggtggtg gtaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    14641 gccgccagcc cgtagttcca ggccacaccg ccagtcgcca gcagtaaacc gccaatcatg
    14701 ggcgaaatca ccgatcccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    14761 ttttcacgcc ctaccagtgc tggtgtcgcc gccagtagcg ccgtaacgcc aagtgatgcg
    14821 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagt
    14881 gcattaaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14941 tttttgcgct catagcgatc cgccagcaca ccgccgacca tcaggccaac aaacatcgcg
    15001 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat tatctggatc
    15061 tgcaccggta ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    15121 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    15181 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    15241 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    15301 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    15361 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct
    15421 ggaggccctc tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcggct
    15481 gccgcgcacc cttgccggtt tactggcagg cggcgcgctt gggcttgccg gggcgttaat
    15541 gcaaaccctc acccgaaacc cacttgccga ccccggcttg cttggcgtaa acgccggagc
    15601 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    15661 ggcgatggcc ttcgccgggg cgctggtggc ctcgttgatt gttgccttta ccggcagcca
    15721 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15781 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15841 tttctggcaa gccggttcgc tggatattcg caatctatat accttaaaag ttgtgctgat
    15901 cccggtgctg atcgccggag caaccgcgct attactgagc cgcgcgctga acagtttaag
    15961 cctcggcagt gacaccgcga cagcgctggg cagccgcgtg gcgcgcacac agttgattgg
    16021 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    16081 tattggcctg atgatgccgc atatggcgcg ttggttggtt ggagcagatc atcgctggtc
    16141 gctgcccgtc acgctacttg ccacccctgc cctgctgctg tttgccgata tcatcgggcg
    16201 ggtgattgtg cccggcgaac tgcgagtttc cgtggtcagt gcgtttattg gtgcaccggt
    16261 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggcgca tgatttacgt ctctcgccga
    16321 ttaattatca cctgtttgct gctggtttcc gcctgtgtgg ttgcaggtat ctggggatta
    16381 cgcagcggtg ccatcacgct ggaaacctcg caggtattcg ccgcgctgat gggcgatgcg
    16441 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    16501 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    16561 ctcggcagcc ctgacgtaat gggctttaac accggggcat ggagcggcgt actggtggcg
    16621 atggtgctgt ttggtcagga tctgacggct atcgcgctgg cagcaatggc ggggggtatt
    16681 gtcacctcgc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    16741 attattatcg gtatcggtgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg
    16801 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaacggc
    16861 ctgacgtggg caaaaacctc accttccgcg ccaatcatta tattgatgct cattgccgcc
    16921 tccttgctgg tgcggcgaat gcgcttgctg gaaatgggcg atgataccgc atgtgcgctg
    16981 ggcgtcagcg tcgaacgttc gcggctgtta atgatgctgg tcgccgtagt gcttaccgct
    17041 gcggcaacag cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    17101 cgccgcatta gtggcaccgc tcgctggggg ctaacccagg cggcgctgtg cggggcgttg
    17161 ttactgctgg tggccgatct ctgcgcccaa ctactgttta tgccgtatca acttccggtt
    17221 ggcgtcgtta ccgtcagcct cggtggtatt taccttatcg tcttgttaat tcaggagtct
    17281 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctcggatatg
    17341 gcaaatatac cgttgcggaa aatctgactg tagaaatacc tgatggtcac ttcacggcaa
    17401 ttatcgggcc aaatggctgc ggtaaatcca cgttgctgcg taccttaagc cgcctgatga
    17461 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    17521 aggttgcacg ccggattggc ctgttggcgc aaaacgccac cacgccggga gatatcaccg
    17581 tgcaggagct ggtggcgcgt gggcgttacc cgcatcaacc gctgtttacc cgctggcgca
    17641 aagaagatga agacgcggta acgaaagcga tgcaggccac gggaatcact catctggcag
    17701 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggata gcaatggtgc
    17761 tggcccagga aacggcaatt atgctgctcg atgaacccac gacctggctg gatatcagtc
    17821 atcagattga tttgctggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17881 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17941 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgatcg
    18001 agcgcattta tggtctgcgc tgcatgatta ttgacgatcc ggttgccgga acgccgctcg
    18061 tggtgccgct cggacgaacg acaccttcaa ccgcgaagat ttaaactaag tggtctgcca
    18121 ttatggcatc ctgttttctg gatgccatcg catggcgcaa tagcacgcca ccacaagcca
    18181 ctataccgcc gatcaacgca gaaaggatca caataatcgc cttacccgga ccgtcttttt
    18241 ttacgggtaa tgacggacgc aactgatatt taaacggtgt aaaattcaca tcgttgacat
    18301 ttgtttttgt taattgctcg actagatact gacgattacg taattcaccg ttcaattccg
    18361 caacgtcagt gactgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    18421 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    18481 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    18541 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    18601 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    18661 tatacccggc caacactttc tgtgcctctt cactggtggg tgcggtgaaa cttagcgtcc
    18721 aggaagtata caatgcagac tcatcttttt tcttactggc attgtcatca accgctttca
    18781 ttttttcgct caatgcgaca attgcgcgat gcaaatccag ttcgtcgatt ttcgcctctt
    18841 ttaattggtc catcacataa ggtgttgagc gcaggtactc ttccagcaag ctaaccgact
    18901 gaaacttctt gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca
    18961 gcacacgaag cttagtgaat gttttctcaa gcgcctgcca ctgaacaggt tctggaggcg
    19021 tgacaaccgc cgcgctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    19081 aggcaaacgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcaatta
    19141 gattaagtaa atcaacttca ttattactgg gcgacgccag aggatactcg ggaaaatgag
    19201 cttcacttcc ctgtttaata ttcagtgatg acatggggta taacctgaaa atgagtccaa
    19261 taagagtaaa tctccacggc ccccactcta ccagttggtg agatttatcc gaagctgaag
    19321 tttgtaaatt actcccactg a
//
LOCUS       DAZGTJ010000089        19257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000089 DAZGTJ010000000
VERSION     DAZGTJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.089"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(133..2799)
                     /gene="cas3"
                     /locus_tag="ACHK8E_003932"
     CDS             complement(133..2799)
                     /gene="cas3"
                     /locus_tag="ACHK8E_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFP0346138.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            complement(3158..3892)
                     /gene="cysH"
                     /locus_tag="ACHK8E_003933"
     CDS             complement(3158..3892)
                     /gene="cysH"
                     /locus_tag="ACHK8E_003933"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0346139.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(3966..5678)
                     /gene="cysI"
                     /locus_tag="ACHK8E_003934"
     CDS             complement(3966..5678)
                     /gene="cysI"
                     /locus_tag="ACHK8E_003934"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP0346140.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5678..7477)
                     /gene="cysJ"
                     /locus_tag="ACHK8E_003935"
     CDS             complement(5678..7477)
                     /gene="cysJ"
                     /locus_tag="ACHK8E_003935"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP0346141.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7796..8158
                     /gene="queD"
                     /locus_tag="ACHK8E_003936"
     CDS             7796..8158
                     /gene="queD"
                     /locus_tag="ACHK8E_003936"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP0346142.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8236..9507
                     /gene="ygcN"
                     /locus_tag="ACHK8E_003937"
     CDS             8236..9507
                     /gene="ygcN"
                     /locus_tag="ACHK8E_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0346143.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            9498..9758
                     /gene="ygcO"
                     /locus_tag="ACHK8E_003938"
     CDS             9498..9758
                     /gene="ygcO"
                     /locus_tag="ACHK8E_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0346144.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            9775..10350
                     /gene="ygcP"
                     /locus_tag="ACHK8E_003939"
     CDS             9775..10350
                     /gene="ygcP"
                     /locus_tag="ACHK8E_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP0346145.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10497..11357)
                     /gene="ygcQ"
                     /locus_tag="ACHK8E_003940"
     CDS             complement(10497..11357)
                     /gene="ygcQ"
                     /locus_tag="ACHK8E_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0346146.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(11354..12133)
                     /gene="ygcR"
                     /locus_tag="ACHK8E_003941"
     CDS             complement(11354..12133)
                     /gene="ygcR"
                     /locus_tag="ACHK8E_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP0346147.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12111..13448)
                     /gene="ygcS"
                     /locus_tag="ACHK8E_003942"
     CDS             complement(12111..13448)
                     /gene="ygcS"
                     /locus_tag="ACHK8E_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346148.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            complement(13541..14995)
                     /gene="ygcU"
                     /locus_tag="ACHK8E_003943"
     CDS             complement(13541..14995)
                     /gene="ygcU"
                     /locus_tag="ACHK8E_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP0346149.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15065..15850)
                     /gene="ygcW"
                     /locus_tag="ACHK8E_003944"
     CDS             complement(15065..15850)
                     /gene="ygcW"
                     /locus_tag="ACHK8E_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0346150.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            16169..17446
                     /gene="yqcE"
                     /locus_tag="ACHK8E_003945"
     CDS             16169..17446
                     /gene="yqcE"
                     /locus_tag="ACHK8E_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP0346151.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17473..18951
                     /gene="ygcE"
                     /locus_tag="ACHK8E_003946"
     CDS             17473..18951
                     /gene="ygcE"
                     /locus_tag="ACHK8E_003946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP0346152.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4693 a         4977 c         4725 g         4862 t
ORIGIN      
        1 tattcatact gtgaatataa aatctcatac cgggaaatta aaagaagatg tacattgtgc
       61 actttcccta cttaagtagg gataaaccgt tattggtctt gttatcgtca ttgataccaa
      121 tcattcccga agttatttgg gatttgcagg gatgactctg gtcatccctt catcccgagt
      181 gtaggtaata acaatattat taccctgcca gatccatcca tcctgattct gtttcccttc
      241 cagccaaagt aacccatctt catctactac ttcagaaaaa ttacgtttcc aggtgaaggg
      301 tacattgacg cgattaagtg ctaacgcctc atactgctgt tcatgactta ggtcctcgta
      361 gacctggcca tcgagcagtt gtttacctga agacgtttgt aaataaggca ataatggcag
      421 gctcatttcc ccatccctcg ttaccgcaag aattgtttca tcgttatcct gcaagctata
      481 ttcttcagcc cactgcagaa ccttgcgagc cttgaacctc ttttcacact cagcgctttc
      541 aaacttatcc atgcctttga tgacccattc tggctcatcc atttcagcat catcgtaaat
      601 gctatccagc cattgccggt aggcatcggg gaaaaataag gatgctccat taagttcctc
      661 aatatgttgc tgcgtccgcc acatgactct aacgttgcta taaatatgct catgtcgtcc
      721 gtaaccttcg ccatcaggca gcaaaacggt ggcaagagga acctcaaaac cagcgggacg
      781 atatttgcga tgatggcgat gtaaacggcc caatcgttgg aaaagtaaat ctgcggggca
      841 atgctgagta attaaccaat caaaatcaac gtcgagtgat tgttccacga cctgggtggc
      901 gacaagtatc cgtccaacat ttcgcttccc atttttgccg aaattgctaa taactcgatt
      961 ctctttttca cgacgatcgt tcagcgtaaa gcgcgcatga aacaaatcta tatctacttg
     1021 cgtgttattt agttccttta accgttggtg gcatagctgt gcaacatcaa ccaaattgca
     1081 gataagacag acttgtgcac ccgcatttgc cgctacgatc attcgctcta acatcctaag
     1141 gtcaggtaac atgtcagcta aataaatagg ttctggccga atcgaaaagc ggggcgggag
     1201 ttgttctgaa tgagctaaca gatcaaaacg ttgtgcacca ttcacacctc gccagttaat
     1261 gagtggatat gcggagttat tttcctctgg atctgtatgc agaccataag tatccagaag
     1321 tttctgtttt tgtttcattg gtagagttgc ggaaagaaga ataacactcc cccccacatc
     1381 agcctgagcc ttgagcacag cctccagcaa gccgttcata tacgtgtcgt aagcatgaac
     1441 ttcatcaaca attaaaacac ttcgaccaat tcccaaacca cggataaagc ggtgtttaac
     1501 tggcaatacc gatatcaaca cctgatcaat cgtgcaaacg ccgatttgcc caagaaacac
     1561 tttcttattg ctttgtgaca accactgaca acactgaacc catgcttctt cttgcccctg
     1621 ctcagtaaat gcgcgtgatt ttattgattg aaaaaggtgg ttaaaacgtg aattaccatg
     1681 agcaagaata agatttgggg aggtaaataa acggctcgcg ttcgcttcca ttctgctgag
     1741 cattgcattt gcagtagctt gtgtagggag ggcaaaaata acactatccg caagttgttg
     1801 gtcaataagt ttccaggcat aggccagcgc agtttcagtt ttacctgaac ccgtaggtgc
     1861 ctctattacc gtcagcccgg gcgctactgg aagagcatca actaacactt gtaattgtct
     1921 gggttgatag ccattgtcca gtagagcatg aacaccttca taacatcgct tatttgatac
     1981 aaggccgctc aactccaata cccggctcgc atcctgctgt cggtcctgga aatacgttct
     2041 tagagcattt atgttggaag gtgcatcctc tttaaacaga aaggtattcg ttgtagtcca
     2101 ggagcctaac cagtcagcaa gcgagcaaaa acctgctaac agtgatgaac aatcaggtgg
     2161 tatatcgttt atagataacc cggctggcgt taaaaataat gcttcgagta cagatatcca
     2221 ctcctcacga gcctgtttat cttgcgcagc ataagatgcc agagaagctg gcatttccca
     2281 acgcgactta tcttgatcct gggaatgtaa tataaaacca tgatgtcctg taacagcctc
     2341 tacccatgga aaccaggact cataaggatg aggagcggca tcaaaaaaac tgaaaaaatc
     2401 cccgggagat tgctctgaaa gtgaatcctg gttaaaccaa tacagaccgg ctgcaccatg
     2461 attaaattta cggcacattt gtgttgatgg accattaagt gatggcgttg caggatttaa
     2521 tttcagccaa ctttctgctg atttatattg gaatcgtata tcaaactttc cgatatcatg
     2581 aagggcaatg aaaaataaca gccaggcttt caccttctgt tttgatagca tttcatttcg
     2641 gcaaaaagca ttttgtagta cgactgattg atcccaccag caatctgcaa cagctgcaac
     2701 atccaggcaa tgataaatca acagatgaat atcatttcct ttcgtcaggc tttttgagga
     2761 tttcccccag taatgacata tatatttaaa aggttccatt aatagcctcc ctgttttttt
     2821 agtattatcg atgatatcag tggactattt ttcgatgctg ctcataaata tcgaattaca
     2881 gtgatataca catgtcttgc ctccctgcaa agaagagact aacattcgaa ttgcttaggt
     2941 tcgaacgctg gcctcaggtt gatagaaata tcgcctgggg cttttgtcca tctggaacct
     3001 cgcgaatgct taacgccaga cagcctcaag cacccaactc cattctatac ctgttaattc
     3061 tccctgcgat gttcaccttt aaatgccgga atgctgctcg caaaataaaa acgcccccat
     3121 catttctgac agaggcgttt aatttatccg gcaatattta cccttcgtgt aacccacatt
     3181 ccctttttaa gccaaagaaa cgcgtttctt cttccgccat gccgggttcc catttacggg
     3241 ttgtatgggt atcaccgacc gataaatatc cttcgtccca taatgggtga tatttcaggc
     3301 catgtttttg caggtactga taaatagttc ggttatccca gtcgataatc ggcagcactt
     3361 taaatacgcc acgctgaatt gccagcaccg gtaaattggc gcggctgccg gattgttcac
     3421 ggcgcaggcc agcaaaccag gtttgcgcat tcagttcttt cagcgcccgg ttcatcggtt
     3481 cgactttgtt gatctcgttg tacttctcaa tcccctcaac accctgctcc cacagtttac
     3541 cgtagcgcgc ttcctgccag gcggcgcttt cggtagcgcg gtacactttc aggttgagct
     3601 tgagtttgtc cgttaactcg tcaataaagc ggtaggtttc tgggaacaga tagccggtat
     3661 cggtgaggat caccggaata tccgggcgaa tctgattcac cagatgcagg ctcaccgccg
     3721 cctgaatgcc aaagctggaa gaaagtacat attcaccggg cagattatcc agcgcccagg
     3781 caacgcggcc ttcagcgtcc agtttttcca gttcggcgtt agtttccgcc agcgccagaa
     3841 tgcgatctac cttcggcagt tcgttcaggg cgtttagatc gagtttggac atagattcct
     3901 cactgtttgc cttgcctgat gcgacgctca cgcgtcttat caggcctaca agaccgggct
     3961 aatggttaat cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc
     4021 accgtaaagt cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt
     4081 tcatcaagcg acgccaggat ttccggctcg gtgatgtttt ctttatacat ccgtgggata
     4141 cgtgtcccaa tgcggttgcc gccaagatgc aagttgtagc gacctggcgc tttacccacc
     4201 aggcccactt ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc
     4261 acgatatgct catcgctgac accatgtttc gccattaaat tatcgatgtt gtcgataaaa
     4321 gacggcagga aacgctcggc ttcggccatc gccagcgggc aagtcgggaa tgacacgcag
     4381 gccattgagt tttcacgctg cggcgtgacg gcattcatta acccgctctc tttggcgatc
     4441 ttctcgatct tcgctttctc gctttccggc acaccggcga tgatcagatt ctggttcgcc
     4501 gtaatgcgga aatcgccttt gtggatcttc gcgatctcca gcaggccggt tttcagcgga
     4561 cgcccaggat aatcaaggat gcgaccattt tcgataaaca gcgtcagatg ccagttatca
     4621 tcaatgccct taacccagcc aatacgatcg ccgcgtccgg taaactcata cggacggatc
     4681 ggttcaaatt tgatccccgc gcgacgctcc acttccgctt taaacgtctc aacccccacg
     4741 cgctccagcg tgtatttggt tttggcattt ttacgatcgg ttcggttacc ccagtcacgc
     4801 tgagttgtca cgacggcttc cgccactgcc aacgtatgct ccagcggcag atagccaaac
     4861 tcactcgcgg tgcgggcgta ggttttcttg ttgccgtgtt cgatggaaag cccaccgccc
     4921 accaacaggt taaagcccac cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg
     4981 ttggcgtgca gatcgatatc gttctgcggc gggatcacta ccgtggtttt gaacttacgc
     5041 ggcaggtagg tctgaccaag gatcggctct tcatcagtag tggcgacttt ttcctgatcg
     5101 agccagatct ccgcatacgc gcgggtacga ggcagcagat gttcagagat tttcttcgcc
     5161 cattcatatg cttccgcgtg cagttgcgac tcgtaagggt tcgaggtgca gagtacgtta
     5221 cggttcatgt cgttagcagt cgccagcgca tcaaggccga ccgagtgcag catctggtgc
     5281 accggtttga cgttcttttt cagaatgccg tggaactgaa acgtctggcg gttggtcagg
     5341 cgaatgctgc catagatggt gttttcaccg gcaaatttgt ctatcgcctg ccactgtttg
     5401 gtggtgatca cgccacccgg cagacggcag cgcaacagca tcgcgtggcg cggctccagc
     5461 ttctgttcag cacgttcggc gcggatgtcg cggtcatcct gctgatacat gccgtggaag
     5521 cggatcagca ggaagttgtc gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc
     5581 gcaatggtgc cgcgcaggta gttgctttca agcttcatgc gctcggcgtc tgtcagtttt
     5641 ccttcgacca ctaaaggccc tggatgtttt tcgctcatta gtagacatct cgctgataac
     5701 ggcgctctac gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa
     5761 attcggcaat cacttccaga agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc
     5821 cgcagacata aatgtgggca ccgtcattga tccagcgcca cagctccgcg ccctgttcgc
     5881 gcagtttgtc ttgtacgtaa attttttctt tttgatcgcg cgaccaggca agatcgatac
     5941 gcgtcagcac gccctctttg acgtagcgct gccattccac ctggtagagg aaatcttccg
     6001 taaagtgcgg gttgccaaag aacagccagt ttttccccgg cgcctcgtcg gcggcgcgtt
     6061 gctgcataaa ggcgcggaac ggcgcgatgc cggtgcctgg gccaatcata atcaccgacg
     6121 tttccggatt ggctggcagg cggaagttgt cgttatgttc gataaagacg cggacttcac
     6181 catcttcttc cacacgatct gcgaggaagc tggaggcacc accggcacgg gcgcggcctt
     6241 ccacgtcgta acgcaccaca ccaacggtaa cgtgcacttc gttctcgact tccgcctgcg
     6301 aggaggcgat ggaatacagg cgcggggtca gcgggcgcag caggttaatc agcgcttcgg
     6361 catccagttg cgccggagag aaacgcacca tgtcaacaat cggcgtggtc gcggcgtaat
     6421 gctgtaactt cgctttatcg cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg
     6481 cgtagttctc aacaatattc gcggtgttga cggtcagttc aaagtgccac tgtagcgctt
     6541 cgctcagggt cagcgttttg ccgtcaacgg tgacaggttc atcgcctttc agccacagca
     6601 gttcgacaag ttctttcacc agtgccggat cgttctgata ccagacgccc agcgcgtcac
     6661 ccggctggta acgcaggcca gagtcaccta agtcaatttc gatatggcga acgtcttttt
     6721 cagagttacg cccggtaatt ttctggttaa cagagaggct cgccaccagc ggcgcgtctt
     6781 tgctgtacgg gctggtgtgg atttcattta ccgcgccagt agcgacggat tgcgaaggtg
     6841 ccgcgacagg cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac tcgatggcag
     6901 cagcctggta ttcaacgtcg gcatcgacac ggtcgagcag gcgttcaccg cccagttccg
     6961 ccagcttact gtcaaaatct ttcccggact ggcagaaaaa ttcataagaa ctatcgccga
     7021 ggctaaacac ggcaaaagcg gtattttcca gcttcggcgc ttttttggag aacaggaact
     7081 tatgcagcgc gacggcttct tccggcggct ccccttcccc ttgcgttgac gtcactacga
     7141 tgagcagttt ttcgctggcg atttgtttga atttatagtc gcccgcgttc accagcttaa
     7201 cgttcagttt tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc
     7261 cggtttgcga ggcggagata atagttatac ccggcatttc tgcggctggc gctggcgtcg
     7321 ctgcaagcgc agcaggctgc tgattgagta cgccccagaa atagccagaa acccaggcaa
     7381 gctgggtagg agttaaatcg gtcgtggccg cctgaaggcg tgccagttgc tccgggttca
     7441 acggaagcaa cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca
     7501 aagctgtttc tgcgccctgt cagcgcccat aaaacagaag aggaggtaag gttaacgggg
     7561 tgaacggtgt ggattaaaga cgggatagcg ataactaata accaaatcga ctaacctgtt
     7621 ttagcaatag tctttaacaa caaaacagat taaccaaccc aatgaaaaac aaatgaattt
     7681 agccaatcat taagataaat cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa
     7741 aaaacagcct ttccggtact ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc
     7801 caccacgtta tttaaagatt tcaccttcga agccgctcac cgcttaccac acgtcccgga
     7861 agggcataaa tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg aaattaccgg
     7921 ggaagtcgat ccgcatacgg gctggattat cgatttcgct gaactgaaag cggcgtttaa
     7981 gccaacctac gagcgcctcg atcactatta tctcaatgat attccaggtc tggaaaaccc
     8041 gaccagcgag gttttagcaa aatggatttg ggatcaagtt aaacccgttg tgccgctgtt
     8101 aagtgcggtg atggtaaaag aaacctgcac cgcaggttgc atctatcgcg gcgaatgata
     8161 agagtgtgtc ggcggtcaat ttcccttaag taacgctatg ttagggtgtt gtgttctgga
     8221 tatctggggc atgacatgga agacgactgc gacattatta ttattggtgc cggtattgca
     8281 ggcaccgctt gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc
     8341 gctgaaatcc ccggcagcaa aaatctttcc ggcgggcggt tatataccca tgcactcgcg
     8401 gaactcctcc ctcagtttca cctgaccgcg cctcttgaac gacgcatcac tcacgaaagc
     8461 ctttccctgt taacgccgga tggcgcaacg acgttttcca gcttacagcc cggcggtgaa
     8521 tcctggagtg tattacgtgc acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa
     8581 ggtgtcgaat gcatccccgg tgcgacggtg gatgcgctgt atgaagaaaa cggcagggtg
     8641 tgtggtgtca tttgtggtga cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt
     8701 gccaacagcg tcctggctga acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg
     8761 gcgttgggga tcaaagaagt gctgtcgctg gaaacatccg ctattgaaga acgttttcat
     8821 ctggagaata acgaaggcgc agcgttgctg ttcagcggcg ggatctgtga tgacttaccc
     8881 ggcggcgcat ttctttatac taatcaacaa acgctctcgt tagggattgt ttgcccgctc
     8941 tcttccctta cgcaaagtcg tgttccggca agcgagctgc tggctcgctt taaaacgcat
     9001 ccggcagtgc gcccgcttat caaaaacacg gaatcactgg agtatggtgc gcatctggtg
     9061 ccagaaggtg gcttgcacag tatgccggtg caatacgccg gtaacggctg gctgctgctg
     9121 ggcgatgcgt tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg
     9181 ctgactggca cgcaggcggc ggcacaaacg ctgataagcg cctgccagca ccgcgagccg
     9241 caaaatctgt ttgcgcttta tcatcacaac gtagagcgca gcctgctatg ggatgttcta
     9301 caacgttatc agcatgttcc ggcgcttttg caacgccctg gatggtaccg tgcgtggcct
     9361 gcgttaatgc aggatatttc ccgcgattta tgggatcagg gtgataaacc tgttccaccg
     9421 ctgcgccagt tattctggcg tcatttacgt cgtcacggcc tgtggcatct ggcgggcgat
     9481 gttatcagga gtctgcgatg tctgtaaccc gtaatctctg gcgcgttgcc gatgcgccgc
     9541 acattgtacc ggctgactcc gttgagcgtc agacggtaca acggttgatt aacgcttgtc
     9601 cggctggtct tttttcgctc acaccggaag gtgacttacg cgttgattat cgcggctgcc
     9661 tggagtgtgg cacctgccgt ttgctgtgcg atgaaaaaac actacaacag tggcgctatc
     9721 cgccttccgg attcggcatc acctaccgtt ttggataaat aataaggata atttatgccc
     9781 ctcttacacc tgctccgcca gaatccggtg attgctgccg ttaaagataa tgccagcctg
     9841 caactggcaa tcgactctga atgccagttt atttccgtgt tgtacggcaa tatctgcacc
     9901 attagtcaaa tcgttaaaaa gattaaaaat gccggaaaat atgcttttat tcatgttgat
     9961 ttgctggaag gcgcgtcaaa taaagaagtg gtgattcagt ttttgaaact ggtgactgag
    10021 gcggacggca ttatcagtac caaagcctca atgttgaaag cggcaagagc ggaaggtttt
    10081 ttctgtattc atcgcctgtt tattgttgat tcgatttcgt ttcacaacat tgataagcaa
    10141 gttgcgcaat cgaatccgga ttgtattgag atcctgccag gctgtatgcc taaagtgctg
    10201 ggctgggtga cggagaaaat ccgccaaccg ctgattgccg gcgggctggt gtgcgatgaa
    10261 gaagatgcgc gtaatgcgat taacgcgggt gttgtggcgc tttccaccac gaataccggg
    10321 gtctggacgt tagcgaaaaa attgctttga cgggatactc gtaacgagtt gaatttgtgt
    10381 gatttgtagg ctgcacgcca catccgacat tcagcgcctg atacgacgct tgacgcgtct
    10441 tatcaggctt acaagtcccc agcccagaac cgtaggcagg atcatccggc agcacgttat
    10501 tggcaatctg cgtgaatggt tgtcaccagt gcctccagca ccgctttcca gtcatcaacc
    10561 acgccaacat ctgcctgtga aaataccgcc gcgctggcgt catgattaat cgccaccaca
    10621 aacttgctat tgcgtacgcc cgccattaac gctgctgcgc cggaagcgcc aacaacaatg
    10681 cacacctcag gcgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca
    10741 ccgttcatca ctcgcgcccg actgtagccc acttcggctc ccagcttttc taccagcata
    10801 gcgatttcct ggttatctgc ctctccgccc tgccccacca ccagcacccg tctggcttca
    10861 gctaatggat cgcgggtaac atttttcagg ttttctatgc taatcagcca gtcgggaagc
    10921 gcaccgggga caatgttcag ttgcagcata ccagagggca acatggtgtt tatctctgct
    10981 cccgcctgac gcgccaggga aagacatagc gggcgttttt ctgtttgcag cgtcgccgtt
    11041 agcgcatttc cccagtggga tttgcgcacg cttacggttg gaatatctaa cgaagtcacc
    11101 tggcagatgc ttgcaccatg taaacgccag gccagccgga tcgacagttc atcgccaaac
    11161 gcgcccggag gaaaaaggac aacctccgca ggcgttcgtt gccactgttc caccagcgca
    11221 tccagcacct gttccgcaac catcggctgt ggttctattt gccaatgcgt tagtgtgctg
    11281 ccgctgaaat cctgcgccgc cagccagccc gccattgcgg catgttcctg atttattgtc
    11341 acaattgcga tattcatggc tgcatccttt ggcgtaaata gtcctgccat agcttttgcg
    11401 ctttttctgc taccgtttgc ccatcaatca gcgccgctct gcgtcgttgc tctggccttg
    11461 ccagttgcag gcactgtatc gctggcgctt ccgcggcaac agtttcgcgg gtaatctccg
    11521 ctttccccgc tgccattcgc tggcgcattc ccggtactgg caaggccact tcaccacact
    11581 gacgcacggc aatcaccgca ggcaagcgaa cccggcaaca gcgcaaccca tgttcagtac
    11641 gctgttcgag ggtgataaac ggcgcgtcga gagtaaaacg ctccacctgg gtgaaacagg
    11701 gccagcccag catttccgcc agcaaaaatg gcgtttgtcc attctgccct tcgctgcttt
    11761 ggcagccggt aatgatcaga tccagcggat tctgacgctg ccattcggca atatggcgag
    11821 cgacaaattc cggcgcaaag cgcagatctg ccgccgtctc cagcaataca gcgtcctcaa
    11881 acccaagagc catgagatag cgcagccagt gcaacgcccg ttcatccccc atgctcaacg
    11941 cggttaaaga catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcggcagcct
    12001 gttcatcggc accgagtaaa cttcgcagaa gcgaaacatc cggtccgata ttaccctgag
    12061 ccgccgcctg ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta
    12121 acagaatgtt cattcgcacc tcctacattt cccgccgcca ccagcgggag tgctttggtt
    12181 tctggtgccc atagccaggt cacaacaaaa ccaaccaaca acactgccgc caggagcaat
    12241 aaggtggctt gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc
    12301 gcccccaacc gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga
    12361 aaactttccg caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag
    12421 agaacaaaaa gcagcagcgt taatgaactg ccggaaggca aacaggccat cacgaccagc
    12481 gttgccgcca gcagcaaaaa acttcccagt aaaaatctgc gatgcgctaa caagtgggtc
    12541 aacactaacc ctaataacgc gccaacaatt aacaacgcat taagcatcag gctggcagtc
    12601 agcgcatctt ccagaccaat agtttgggcg atggtcggca gccaggtata aatcacaaac
    12661 catgggatta cgaggcagac aaagaagacg ctgttaaacg ctgtgcgccg ccagtagcgc
    12721 gaagagaaca aggttttgat gtgtttatgg gtcgccgttg ccacttcatc gcccagtaaa
    12781 acatggggac caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc
    12841 agtagccagc gtggcgattc tggcgttccc cagcgtaata acgtaatcaa caatgcgggc
    12901 agagctgccg atgccagtag ccagcgccag gcctccgggc tttcgctaat aaagtgatgt
    12961 ccggcaatac ttgccagcac atagcctacg gtccacacca cgctgaatgc gcccagcaaa
    13021 acaccgcgat ggcggcgcgg agaaaattca gccagcaaag tgtgaccgac tgagtaatcg
    13081 cctcccagac caatgccgat caaaatgcgc agtccaataa gatgctctgg cgtggtggca
    13141 aaaaattgta agaacgaagc gagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc
    13201 cgaccaatat ggtcggagat ccaccccaaa accaggctac cgataaacag gccaagtagc
    13261 gccgagccgc cgatcattcc cgccataaac ggcgtcagtt gcatggcggg tgtaagctga
    13321 ataatggcgt aaccaatgac gccgaggaca taaccgtcgg tcaggtgtgc gccgaaagtg
    13381 agcgcagcaa tgcggcagtg aaaacggtta agtggtaaat catccattcg caccggtgaa
    13441 gtgttcatct ttaccccttc attgtccggt aataagaacg gcttctcgcc tgagaagccg
    13501 aaaaggcaac gcccgtcatc gcggaacgct gcctgacaca ttatttttca atcggataga
    13561 tagtacccgt attcataatg ccgttaggat cgaactgctt tttcagccct tccagcaacg
    13621 cccacgcgct gccgtgttcc agtttgctcc agtgaacgcg atgtttaccg ataccgtggt
    13681 ggtgcaccat cgaaccaccg aggcgaatgg tttcttcgca gatgattttg ttgagcggat
    13741 tgtggtactt gtcgatttcc tcttccggct tacagtcaac gacgttgtag tcgtagacga
    13801 agtacatgtt ggtgccgttc tgatagctat gagaggagtg accgcccagc atggtgatgt
    13861 cgtcggcatg cgggaactcg gtacgaatac ggttaataac gctttcgtag atttcgtgga
    13921 tgcagctcca gcagccggac acttcggtgg taaagcccat gttgccggtt ttgaggatct
    13981 gcacacgttc agcggccact ttatccggcc cccagttcag gtgattaaac caggtttcga
    14041 tcagcttgct gtccactcgc tggcattgcg ggtaacgtgc cacgatttca gcaatccctt
    14101 cgcctgtcgc cttcgcaatg cgagggttac cttcagccat aaagatcagc acacatttac
    14161 catcggcaaa atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg
    14221 acggacgata cccttccacc atgatttcac gcaggatgtt gaagccggtt ttcatgtctt
    14281 ccagcacata gccatagaag agattgtttt ccggcgtaaa tttgaagatt ttcactgtga
    14341 cttcagtgat atagcacaat gcaccttcgt tgccgatgat aatgtgacga atgtccgggc
    14401 ctgccgcgcg gcgtggtacg tttttaatac gtgtgacggt gccatcagcc aatactgctt
    14461 ccagaccaac gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc
    14521 gggttgctac caggccgccc atctgcgcca gcggttttga ttgcggtgaa tgccccgtgg
    14581 tgtaaccttt ttcacgcaac gtgttttcca gcacctccag cggaacacca cattgcgccg
    14641 tgacctgcat attctcaata tcaatattaa tgatttgatt catggcagag ccgtcgagca
    14701 ccaccgagtt ttccacaacg gtttccagtc caccctcggt agcggaagca ccggtacgcg
    14761 gcacaccgtt aattttgtgt gcattcataa aattcagtac acgagagact tgttctgtgg
    14821 aaccgagttt tacgaccgct gccggaatcg gcaaagtata aatgccatga atatccggaa
    14881 atttgcgaaa acggtcaata ctgtttttct ttaatactgt ttcatcggta attacgcgat
    14941 ctgcaccaac aatttccttt agctggtcga caatcgctgc gcgagataaa gacatagtaa
    15001 ttccttcctg ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta
    15061 tggattagcg cactaaataa ccgccatcga ccactaataa atgcccgttg acataattcg
    15121 atgcctgact tgcgagaaac acggctgcgc ccattaaatc ctgagtatcg ccccaacggt
    15181 ttgccggaat atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagcg
    15241 taatatctgt tgcataatag ccaggggcaa taccatttac ctgaatatta tattgaccta
    15301 gttcatcaca ataagctttg gtgaacccgg caagagcatg tttagtggca gaatatgcag
    15361 gtgaccattg tccacctaag taagagaaca atgaacagat attaataatt ttaccgcttt
    15421 tctgcgggat cataattttt gccgcttcat agcttaactc gaatgcggcg gtcaggttca
    15481 catcaatcat cgggtcccag tcggcacgac cgaaatccag caccttattc agcttacaaa
    15541 taccggcatt gttaaccaga atatcaactg ttccgaaacg ctcacagcaa gcagcgataa
    15601 tcttctgcgg cgcgccttct gcggtgatat ccacctgcat gaagtccatc tcaacaccct
    15661 gtttttcaat catttccttt gttttgccgt tatctttaac gaagcttgga ataaagatat
    15721 ttgcaccagc tttggccagc gccatggcaa atgcctggcc taaaccgcta ttcccgccgg
    15781 taacaattgc ggttttacct ttcagggaga aaaaatccat tgagaacgca ttgagagatt
    15841 cgattgacat agttagctcc aatttccgta aggcaaaaaa aaagaaaggt aacttccccc
    15901 gcgagaggga agttaccttt ctcattatct ctggtaactg gagataattt ttatcataag
    15961 ggcataatcg ctaatgtgat cgtaatcaca gtgttatatt cgaattgtca aataatttta
    16021 aaaagcagaa gcgtgattac tctcacacaa caccactgct ttccatgtta tttcttattg
    16081 caattactag agacctgaga aaagtaatcc acctgaaagg gcggcttact tttctctttt
    16141 ttttgtctat tacaaatggg aatacgatat gcaacacaac tcatatcgcc gttggataac
    16201 cctcgcgata attagtttta gcggcggcgt tagtttcgat ctggcttatt tacgttatat
    16261 ttatcaaatt cccatggcga aatttatggg attcagcaat accgagatag gtttaataat
    16321 gagtaccttt ggtattgcgg ctattattct ttatgccccc agcggcgtta ttgccgataa
    16381 attttcacac cgcaaaatga ttacttccgc gatgatcatt accggattac tgggtctgtt
    16441 aatggcaaca tatccaccgc tgtgggtaat gctctgtatt caggtcgcct ttgcgataac
    16501 gacgatttta atgctgtggt cggtgtcgat taaagccgcc tcattgctgg gcgatcatag
    16561 tgagcaaggg aaaattatgg gctggatgga agggctgcgc ggcgtcggtg taatgtcgct
    16621 ggcggtgttt accatgtggg tcttttctcg ctttgcgccg gatgacagcg ccagcctgaa
    16681 aacagtcatt attatctaca gtgtggttta catcttgttg gggattctgt gctggttttt
    16741 tgttagcgat aacaacaacc tgcgcagtac caataacgaa gaaaaacagt cattccagct
    16801 tagcgatatc ctggccgttt tgcgtatcag taccacctgg tattgcagca tggtgatttt
    16861 tggtgtcttc accatctacg ccattctgag ttactccacc aactatctga ccgaaatgta
    16921 cggcatgtcg ctggtggcgg cgagctacat ggggattgtg atcaacaaaa tcttccgcgc
    16981 gctgtgcggc ccacttggcg gcattatcac cacctacagt aaagtgaaat cccctacccg
    17041 cgtgatccaa atcctttccg tactcggcct gctggcgtta actgccctgc tcgtcacgaa
    17101 ctctaacccg caatcggtcg cgatggggat tggcctgatt ttactgctgg gattcacctg
    17161 ttacgcctca cgcgggctgt actgggcctg cccaggcgaa gcgagaacac cgtcttacat
    17221 tatgggcacc acggtaggta tttgttcggt gattggattc ctgccggatg tcttcgttta
    17281 cccaattatc ggccactggc aagacaccct gcccgcagca gaagcctacc gcaatatgtg
    17341 gctgatgggc atggcggcgc ttggcatggt gattgtcttt acctttttgc tgttccaaaa
    17401 aattcgtact gctgatagcg cccccgcaat ggctagcagc aagtaagcca ggaaaattac
    17461 aggagaaaaa tcatgtcgaa aaaatacatc atagggattg atggcggaag tcagagcaca
    17521 aaagtggtga tgtacgatct ggaaggtaat gtggtttgcg aaggtaaagg cttattgcag
    17581 ccgatgcata cgccagatgc cgatactgcc gaacatcctg acgacgattt atggacatcg
    17641 ttatgctttg ccggtcacga tttgatgagc cagtttgccg gaaataaaga agatattgtc
    17701 ggtattggtc tgggctccat ccgttgctgt cgtgcgttat tgaaagccga tggcacgccc
    17761 gctgcgccat tgattagctg gcaggatgca cgtgtaacac gtccctacga tcataccaat
    17821 ccagatgtgg cctatgtcac ctctttttcg ggttatctga cgcatcgctt aaccggcgag
    17881 tttaaagata atatcgccaa ctattttggt cagtggccgg tggattataa gagctggaca
    17941 tggagtgaag atgctgcggt aatggataag tttaatatcc cccgtcatat gctgtttgat
    18001 gtgcaaatgc ctggcaccat tcttgggcat atcacaccac aagctgcgct ggcgacacat
    18061 ttcccggctg ggctgtcggt tgtttgtacc accagtgata aaccggtaga agctctggga
    18121 gccgggttac tggatgatga aactgcggta atttctcttg gcacttacat cgcattgatg
    18181 atgaacggca aagccttgcc gaaagatccg gtggcgtact ggccgattat gtcttctatt
    18241 ccgcaaacat tgctgtatga aggttacggt attcgcaaag gcatgtggac ggtgagctgg
    18301 ctgcgcgaca tgttaggcga gtcgttaatt caggatgcca gggcgcagga tctttcaccg
    18361 gaagatttac tcaacaagaa agcctctagt gtgccacctg gctgtaatgg gctgatgacg
    18421 gtgctggact ggctgaccaa tccgtgggaa ccgtacaaac gcgggattat gatcggcttt
    18481 gattccagta tggattacgc atggatatat cgttcgatac tggaaagcgt ggcgctgacg
    18541 ctgaagaaca attacgacaa tatgtgtaat gaaatgaatc actttgcgaa gcatgtaatc
    18601 attactggcg gcggttcgaa cagcgatctg tttatgcaga tttttgccga cgtgttcaac
    18661 cttccggcac gacgtaacgc cattaacggt tgtgcaagtc tgggggcagc gattaatacc
    18721 gcggtaggtc tggggctata cccggattac gcaacggctg tcgacaaaat gattcgcgtg
    18781 aaagatatct ttataccgat tgagagcaat gccaaacgct acgacgcgat gaataaaggc
    18841 attttcaaag acctaaccaa acatactgat gtgatcctga aaaaatcgta tgaagtgatg
    18901 catggggaat tggggaatgc ggattcgatc cagagctggt cgaatgcgta agtgaggaag
    18961 gccgggcggg aaactgcccg gcctgaacat acctgaatgg ttatccccgc tgacgcgggg
    19021 aacataagtt cgacaactta aaaaatgata acaagtccgg tttatccccg ctgatgcagg
    19081 gaacataata aaaactttaa ctagttcggt ttatccctgt ttttacaagg aatattaact
    19141 acactcgatg tctttagaat aaaagacaat acgaatcgta ctttatcaat tttcatttaa
    19201 agtatgaact ggatatcttt attcataaga acaattcatc aacagcgtat agaggcg
//
LOCUS       DAZGTJ010000090        18781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000090 DAZGTJ010000000
VERSION     DAZGTJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.090"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            169..1227
                     /locus_tag="ACHK8E_003947"
     CDS             169..1227
                     /locus_tag="ACHK8E_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0346153.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTTHAPSKPESFLKRLFSAGQTDNTDNTDTSKPDTGEDAKMDEIKALLEKLITLVE
                     GGSAAAAGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTA
                     AKAKLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGI
                     TPRPAGTPAESEKPFDFV"
     gene            1239..2258
                     /locus_tag="ACHK8E_003948"
     CDS             1239..2258
                     /locus_tag="ACHK8E_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0346154.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEEA
                     EDVGA"
     gene            2276..2974
                     /gene="gpM"
                     /locus_tag="ACHK8E_003949"
     CDS             2276..2974
                     /gene="gpM"
                     /locus_tag="ACHK8E_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP0346155.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNVA"
     gene            3082..3555
                     /locus_tag="ACHK8E_003950"
     CDS             3082..3555
                     /locus_tag="ACHK8E_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0346156.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVDEVNTTLAPVVAHWKAQGHERAADVPGARMGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            3552..4025
                     /locus_tag="ACHK8E_003951"
     CDS             3552..4025
                     /locus_tag="ACHK8E_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0346157.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            4022..4732
                     /locus_tag="ACHK8E_003952"
     CDS             4022..4732
                     /locus_tag="ACHK8E_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346158.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            4744..5889
                     /locus_tag="ACHK8E_003953"
     CDS             4744..5889
                     /locus_tag="ACHK8E_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFP0346159.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            5891..6352
                     /locus_tag="ACHK8E_003954"
     CDS             5891..6352
                     /locus_tag="ACHK8E_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFP0346160.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            6357..6662
                     /locus_tag="ACHK8E_003955"
     CDS             6357..6662
                     /locus_tag="ACHK8E_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFP0346161.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            6652..7404
                     /locus_tag="ACHK8E_003956"
     CDS             6652..7404
                     /locus_tag="ACHK8E_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0346162.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            7404..7742
                     /locus_tag="ACHK8E_003957"
     CDS             7404..7742
                     /locus_tag="ACHK8E_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP0346163.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            7739..8125
                     /locus_tag="ACHK8E_003958"
     CDS             7739..8125
                     /locus_tag="ACHK8E_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346164.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            8225..8500
                     /locus_tag="ACHK8E_003959"
     CDS             8225..8500
                     /locus_tag="ACHK8E_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFP0346165.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            complement(8464..8691)
                     /locus_tag="ACHK8E_003960"
     CDS             complement(8464..8691)
                     /locus_tag="ACHK8E_003960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346166.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            8690..10876
                     /locus_tag="ACHK8E_003961"
     CDS             8690..10876
                     /locus_tag="ACHK8E_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0346167.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTLALTKVRSVIIATSLAARLAGVSFLT
                     MGAPVLLVVAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGDIARAL
                     APVWQGIKTLFGAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALGVVIS
                     VIRNLIDIFTIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETTVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            10873..11223
                     /locus_tag="ACHK8E_003962"
     CDS             10873..11223
                     /locus_tag="ACHK8E_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFP0346168.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            11201..12385
                     /locus_tag="ACHK8E_003963"
     CDS             11201..12385
                     /locus_tag="ACHK8E_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0346169.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLDVTAYIRSSTTLPPDGAEGLKQDIGDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            12378..12926
                     /locus_tag="ACHK8E_003964"
     CDS             12378..12926
                     /locus_tag="ACHK8E_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0346170.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAARAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     TPFIIHYGAYEGEYICWTARAE"
     gene            12949..15015
                     /locus_tag="ACHK8E_003965"
     CDS             12949..15015
                     /locus_tag="ACHK8E_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0346171.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLAAMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELTEDTPMVTPTSPSR
                     IWLNVWWTGTLTSEWTVEHEIIPADTDVTEERLRKTYPRDGIQQYFYAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLE
                     SPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPAGYAIMVGQTFDKARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDG
                     SSSGSHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHGLTINPTGNAENTVKNIAFN
                     YIVRLA"
     gene            15015..15611
                     /locus_tag="ACHK8E_003966"
     CDS             15015..15611
                     /locus_tag="ACHK8E_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0346172.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWQLMEDHRGKTVWRLESGSALLITESGPLPENVTIIKP
                     DGEFCRWDGSSWAKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            15623..16348
                     /locus_tag="ACHK8E_003967"
     CDS             15623..16348
                     /locus_tag="ACHK8E_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346173.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            16320..16862
                     /locus_tag="ACHK8E_003968"
     CDS             16320..16862
                     /locus_tag="ACHK8E_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346174.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARATPASSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            16865..18538
                     /locus_tag="ACHK8E_003969"
                     /pseudo
     CDS             16865..18538
                     /locus_tag="ACHK8E_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18575..>18781)
                     /locus_tag="ACHK8E_003970"
     CDS             complement(18575..>18781)
                     /locus_tag="ACHK8E_003970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346175.1"
                     /translation="CGDFGAQKSFSDVRGSNRAGGIISSLRGVTRRFTWGLRGAFRPS
                     FFLLRTVFSWRVMSGDEYFLLSGE"
BASE COUNT         4455 a         5498 c         5465 g         3363 t
ORIGIN      
        1 atgcgcccat tatggcggcg catccggccc gcattcaggc agaaaatttc ggctgtgttc
       61 ggctatccca cgttatccga agacagccga attcagccag tagccccccg ctgtaaaaat
      121 cccgacactc accggagttc ttcagatttt cacgtaaaag aggccacgat gccgcagcca
      181 acgttgatga ccgactggat atgtatcgcc acatcaggac cgaccgttga cggccgcgaa
      241 attgatccgc agtggctgac tgatgccgct gaaacctatt cccgcaacac ctacacggcg
      301 atgctgtggc cctggcatga ggaagaaatc ggacaccgcc agtacaccag taatctgggt
      361 gaagtggact cactgaaagc ggaaaccggc gaggacggta aaacccgcct ctacgcgcgg
      421 ctggtcccta accagtacct gattgaagcc aacagactgg ggcagaagct gttcacatcc
      481 gccgagatag tggagaactt cgcccggagc ggacgcgact atctcctggg cgtggcggtg
      541 acggatatcc ccgccagtct ggggacggaa aaaatacgct tcaccctgaa caacgaggaa
      601 tacacagcac agcgggcgaa ctgccagacg ttcacgctgg gcagcctgtc accggacacc
      661 acccatgccc ccagtaaacc tgaatcattc ctgaaacgcc tgttcagcgc aggacagacc
      721 gacaacaccg acaacaccga taccagtaag cctgatacag gcgaggacgc aaaaatggac
      781 gaaattaaag cactgcttga aaagctgatc accctggtgg aaggcggcag cgccgctgcc
      841 gctggcgagg gcacagcaga cacgccggaa gaagcaaaag ccgaagtcgc ggaaatcgcc
      901 gccgaaatcg ccgacgccgc cgcagaggtg gcagagctgg cagaagaagt gaaagccgcc
      961 ccggaagacg aggttaaggc cgaagccttt accgctgcaa aagcgaagct ggaagacgcc
     1021 atcaaagcct tcagcaccat cacaccggaa aaaggcgaac gttaccgccg ctttgccgcc
     1081 cgtcgccggg acgacaccga caaagaagaa atcaaagcca ttgccgaaaa acttaacgac
     1141 ctgaccacga agctgtcagc caccggcatc acgccacgcc cggctggcac accggcagaa
     1201 agcgaaaaac cgtttgactt tgtttaagga cacgcaacat gcaactgaac cagaaaaccg
     1261 aatcactgct tcgtaagtac gctgccgggc tgggcgcggc aaacggggtt gacgacgtca
     1321 cacgctattt tgcgctgtct gacccgaagg agaccgcgct gcgtgacgcc atcatgcact
     1381 ccgacgagtt tttatcaaaa ctgccgaacg tgctggatgt tgaacaggtg acaggccagg
     1441 tggttaccac cggcgttccg ggcctccata ccggacgtaa aaaaaatgcc cgcttcaccc
     1501 ttccgctggg tgttaccggg aatgaataca agctggctga aaccgattcc ggcgcccacc
     1561 tcctgtacac cacactggtc acctgggcga acgcaggaag tgaaaacgag ttctttaacc
     1621 gcattcagaa tttcgttaac agacagattg cgctggatat cctgcgcatc gcctttaacg
     1681 gtaccagcgt agccgaagat accgacccgg taaccaacaa aaacggcgag gacgtcaaca
     1741 ccggctggca tgccatcgtc aaaggccgca gcgcaaaaca gatcgtggag gctggcccga
     1801 cgctgggcaa gggtggcgac tttatcgggc tggatgcagc cgtggcagac ctcgtgcata
     1861 ccctcattga tgagtcgtat cgcggtcatc cggatctggt ggtactggtt gcgcccgatc
     1921 ttgtggcaca ggacaaggtg acgctgctta acgccatcga cgcccccacg gaaaaagtgg
     1981 ccgcgcagct tatcggtcgc cagatcgccg ggctgacagc atacaccccg ccgttcatgc
     2041 cggaagggcg cctgattgtc accacgctgg caaaccttca catctacacg cagcgcggca
     2101 cccgtcagcg ccgtgcggag tggaacgacg atcgcaagcg ttttgaaaat aactatctgc
     2161 gcatggaagg ttacgccgtg gagcacgacg aaatttacgc ggcctacgac aagctgacca
     2221 tcaagggcgc agaagaagcc gaagacgtcg gcgcataagc acagagggcg gcgcaatgac
     2281 accctgccag cgacaccggg cgaaaatccg cacacaggag gcactcgaac gccgggaggc
     2341 gctgacggcc tccccggtca gcttccacct gttacgcgcg gagcttgatc gcgatgtggc
     2401 acggctgcgc agtcttccgg tccgtgaaga gcggctggcg ttcaagcgcg acattctcct
     2461 gccgcgctgg ctgcccgtcg cggagcgcta tatcgctgac ggaaagcgcc acgcctgccc
     2521 ggtactggtg tactgcatca tctggctgtt cgacacgggc gacctgagtc gcgcccttga
     2581 ctgggcagac attgccatca gtgaaggcca ggccacgccg gacaacttcc gtagcaccct
     2641 gcccgccttc gtggccgaca cggtgctgca gtgggccacc gacaacagcg aggccggaca
     2701 cagcatagag ccgtattttt cgcgcacctt caggaacgtc accaccacgt ggcggctgca
     2761 tgaggaaatc cgcgcgaagt ggttcagatt tgccgggctg tatcttctgc gcgacgaggc
     2821 gggcaaaccg agagccaccg cgattaatga tactgccgta ctggaaaagg cagacgcata
     2881 cctggcacag gcggcagcac tgagccgtac cgctggcgta aaaagtatca gggcgcgcat
     2941 cagggcacgc atcagcgccc ttaatgtagc gtaaacgact acccgcaggg ccgggcgggc
     3001 gcgggaggag gcatcagtga cggaaactgt atggccgtgg aatcccggac agcccgcctt
     3061 tttattcata tgaggcacgt catgagcgaa ccgagcttca gcgtcagcgg taaaccgctg
     3121 aaatccacac cgcagacggt gacaaacggc gagccgttct ggccggacct tgatctggct
     3181 gagcttcagg ggtcacgcac gcttccggct gatttacccg acgacacggc ggccatggcg
     3241 ctgcttgccg cagtggacga ggtgaacacc acgctggcgc cggttgtggc gcactggaag
     3301 gcgcaggggc atgaacgcgc cgcagacgta ccgggggcca ggatgggcga tgaaacccag
     3361 ctggtcgcac agtacaaaaa ggccgtttat gcccgtgcca aggccgatct tatgggcgag
     3421 ttcgccacca ttggtcgccg tgaaacctat ccggggcagg aaagcgagga cacacgcgcc
     3481 accttactgg ctgaagcctc gctggtcatg cgtaacatgc tggggcacag acgcgcagga
     3541 gtgcacatca tatgaaccag ctggaaagcc tgaccgcctt cattaccgaa aatctgccgc
     3601 ctgacgccat gcagttcttc atgagcagtc aggaagacgc gcagcttatc cgcagtcaga
     3661 aggcgctggg taacgaccag atccgcatgg gcgtgatgac gtacaccggc gtgattgcat
     3721 gggataactt cccgttccgt aaatactcac cggggctggt ttacgcgctg gtcatggcct
     3781 ggctggaaga gcacgcggac ccactgaggg aggaattccg cccggaggat gtgaacgtcg
     3841 acccgcagtt taacgatgag gactcgtgcc ttctcgatat cacggtggga ctggctgacc
     3901 cgctgatcat ccgggtgcag gaagacggcc ccgttccgta cagggggaaa cgctggacac
     3961 tggttaaccc tgaaatctgg acggcggaaa ccatggagct gtttgtcagc aggagccgcg
     4021 aatgataagc ggccagctga acccgcagca gctggcagaa gcgcaggcga tgcttcagtc
     4081 gctggatctg ccaccaaaaa aacgccgtcg cctgttatgg cgtattgccc ggcacggcat
     4141 tatcgccgca gccagacgcc atcagcgcga tcagaccgcg ccggacggca cagcatgggc
     4201 accccggaaa cgcggcaagg ggaaaatgtt cagaaagctg ccgcgtcttc tggcggtccg
     4261 tgagatgccc ggacaggatg ccgtaaaggt ttatttccgt aacggcaatc acaccagtgg
     4321 caggagccgc atcagtgccg gtgccgtggg ctgggttcac cacaacggcg cagaaatccg
     4381 ggtcagtgct gacagcttca gcggtcagga ccagaacggg cagaccgcca cccgccgaca
     4441 ggcaaaacga ctgcgggagc tgggctttaa ggtacgccag aaggggcgct ggcggaaagc
     4501 atccatgaaa tacctgttaa cgctttcgcg ggcgcaggcc gggttgctga tcaccattat
     4561 ggaagatcag attatcgccg gaccacgcca gcagaaggcc cggacatgga ccattaccct
     4621 tccgtcgcgc gtctttctgg gggtaacaaa cgaagaattt tcacacatcc tggcacgcca
     4681 gatgaacgca atgggctacg gcctggacgt tgacgcacag gatatcagat aaggagtcac
     4741 accgtgagct ggccgaatgt aaccgtaagc catcagaacc gtttcaacgg tgccacccgt
     4801 gaagtggagc gcacgctgct ttttgtgggc tatggcaaaa agaacacagg taacaccctt
     4861 tccgtctccc ctgagaccga cctggacgac gtgctgggac cggatgaaag cctgcttaaa
     4921 tccacgctta ccgccgcaat cgccaacggt ggccagaact ggtttgccta tgttcacgtt
     4981 ctgtctgaac ccaaaccccc ggcaccggag ggcggcgacg ctaacgccgc atgggtggat
     5041 gcggtgaaaa aagcgcagac catcgcctct gccgaaggcg tggtgattgc cattgacatc
     5101 acggccaaag atgccgttaa ccgcgccacc gaaacccgcg ccctgcttca gtccgcttat
     5161 ggccgttttg tctggttcat gctgtgcgtg gccggaccgg gcaaggatga ggcatgggcg
     5221 gcatacgtca cccgcatcag tgccatacag gacggcgtgg cagcacctgg cgtgatggtg
     5281 gttccccgct tatggggaaa tgagcccggc gtactggcgg gccgtctctg taatccttcc
     5341 gtcaccgttg cggacagtcc ggcacgtgtg gcaaccggtg ccctggtggc gatgggtaac
     5401 gatgagatcc cgcaggacgg caagaaacag ccccttgagc tggccacgct gcgctcgctg
     5461 gaaggcaaac gcttcagcgt gcccatgtgg tatcacgatt tcgacgggct ctactggtca
     5521 gacggacgca cccttgatgt ggagggtggc gattaccagg tcatcgaaaa cgtgcgcgtg
     5581 gtggataagg tctcccgccg tgtacgtctg cgcgccattg cccgccttgc agatcgcgcg
     5641 ctcaacagca caccgggcag cattgccgcc agccagacat atttcagccg cacgctgcgt
     5701 gaaatggcgc tcgccacgga gattaacggc gtaccgttcc ccggggaggt taaaccgcca
     5761 caggacgggg atatcaccat cgtctggacc agcagtacac acgtgcagat ttacattgtg
     5821 gtacgcccct acgagagcgc aaaagccatt ggcgtaagta ttgaactcga cacagcactg
     5881 gagatctgac atggccggaa atgaacgtat cagcggcggc gcgtttgata tcaactacga
     5941 cgggatcatg atccacgtgg aaagcgccac agtcaccatc accgacaaca gcgccgtggc
     6001 acagacacgc ggcgtgccga acggctacac ccgtggttcc gtttctgccg atgtggaggt
     6061 ggaagtcgac gcgcagaact ttaaaaagtt tggtgctgtg gcccgctctg cggggtcatg
     6121 gcgtgccatt cctgaaaagg attttctctt ttatgccaac aacggcagcg aggaggaaaa
     6181 aatcgaggtg ttcggctgcg tccccatgtt gtcggacgta gtgaacatca gcgcaggtga
     6241 agccggaaag accacgaaaa aaatcaagtt catggtcaca agcccggact ttatcagcat
     6301 tgacggcgtg ccctacctgt cagcccgtga cacccgcgat ctgaaggggt gatgccatgc
     6361 cggacggtca gacttcactc ctggcaaaac tgattatcat cggtgctgcc atcgggctgg
     6421 ggcgtctcat ggtgagccgc gatcgcatca cagcccgtct gcttatcggg cgaatgatta
     6481 tgggctctgc cgtggcaccg gttgccgcca ttccgctgtt taaatttccg gacatgccgg
     6541 agctggtagt ggtcggtctg gcgtgcgcgc tgggcattat gggcagcgcc tttattgaag
     6601 aatgcttttg ccgtctggca gaccgctacc tgaagcagaa ggaacgcaga aatgacccat
     6661 aagccttaca tttcgccttc cctgagcctg tatcacgcgc aggcgcatga cgttctgcgg
     6721 atgctgccgg aaaacagtgt ggacgcgctc atcacagacc cgccatacag ttcaggtgcc
     6781 acacacaagg cggggcggac atgccagggc agccacgcca aatacatcaa cgatgaagat
     6841 cttcaccgct ttgaaggctt cgaaggcgag aacatggacg cccgttcatg ggaatactgg
     6901 acgcatctgt ggatgacaca ggcatggcgc acggtacgac ccggtggtta cgccctggtc
     6961 ttcaccgact ggcggcagtt gcccgcgctg acggacgcat tccaggccgc cggctttacc
     7021 tggcggggga tcgtggcatg gaacaaggga cgggcctcaa gaacaccgca taccggttat
     7081 tttcgtcacc agtgcgaata tgtggtctgg ggcagtaaag gcgcgctggg caaatcccct
     7141 tccgggccgt tcgacggctg catcacgcac cacacacgcc cacaggagaa acagcacccg
     7201 acaggtaaac cggaatcgtt gatggatgag cttgtgcgcg tgtgtgaacc cggagggctg
     7261 atcctcgatc cgtttatggg gtccggcacc acgggcgtgg ccgcccttcg ccggggctgc
     7321 cgttttacgg gtattgaact gaacgcccac tatttcagcg tggcacaggc gcgccttacg
     7381 gggacggaac agaggaatga ctgatgaaac tgagcgaaaa acaacaactg tttgccgtca
     7441 tgattgccga tctcatccac tgggcacagg agcatggcta tcgcctgacc ttcggcgagg
     7501 cataccgcac gccggagcag gccgcactga atgcgaaaag cggaaaaggc attcgcaaca
     7561 gccttcacac gctgcgcctc gcggtggact tcaacctgtt cattaacggc aaataccagg
     7621 ccgataccga cgcctacagg ccgctggggg aatactggga atccatcggc ggcacatggg
     7681 gcgggcgctt cagccgggca gacggcaatc atttcagcct tgagcacaac ggggtgaaat
     7741 gatgaaacag ctggcgggct gcctggtgat cctctttgcc gccatgtggg cgggctggct
     7801 ggcccatgac tggcatgacg ccagggtggc gcttgccgaa caggaagcca tggaagccac
     7861 gcgacgtgcg gtaacagaag ccgggcagca gtccgcccgc atactggaaa aacggctgga
     7921 ggcgttaaag ggcaatgaaa ttcacactga acgcgttatt cacacggaaa ccgtccgccc
     7981 ggtattccgc aatgtgtgcg ctactgatga ttacgtccgg ctgttcaacg cccgtgctga
     8041 agccgctgag cgtaccctgg caggaaaacc agctgacgga atgcccgaag acactgccga
     8101 gactggcagg cccgacgggc gctgattttg atgcggcact gcgaaccctc acgagccgct
     8161 acaccgaatg cgccgccagg cataacagcc tggtggaaac catacgaaac acggagaaac
     8221 tgcaatgacc acgaagaaca ccggcaccat catcctgtcc gttgcgggcg taaccctgag
     8281 ctttgaaccg aacaaaacgg catttaacaa cctcatcaac gaaatcacga tgaccaacaa
     8341 ggtggcgccg atgagcacct atcttaaccg catcgtgacg cctgccagcc gcgacgcact
     8401 ggctgacctg attgcgcgtt atccgggctg tgagatgcag atcgtcgaga agattaacga
     8461 ggtttactcc ccgaaactgg agattgagtt aaaaaactga ccgcgcgggt cgaaagcatc
     8521 cgccgcaatc cccttgagca gtatctggca ttgcggcgtc actacctccc cggagagccg
     8581 gacgacgaag agagcatcgc ccgtgccatc tggctggatg aatattttgc ccgtacccgc
     8641 gccaacaaga ccgctgaagg tatagcgatc gcatttaacg gaaggtgaca tggccagcca
     8701 gctggatttc attatcaatc tgacagacca gcttacccgc ccgctgcggc aggttcagga
     8761 atccgtgacg ggattcgccg aacgctcgca gggcgcgttc gcgcgtatcg gtgtgggtgt
     8821 ggccggactg tggggcgtgg ccgaaggtat ccgcgccgcg atggatccgg cgtgggagat
     8881 gcagaaggcg ctgggcgagc ttgaggccaa aggtgtcggg gcgcaggcgc tgaccgccct
     8941 caccacgcag gccatgaaac tggggaccgc attcggtcag tcttccacgg agatcatccg
     9001 ttccgcatgg catattcgcg gcgcactgga cgggctgagc gacggggatt taccccgtgt
     9061 gacggcagca accagtaccc tggccgtggc tgcccgcgtc agcagcgaag aggcgagcgg
     9121 ctaccttgcc gccatgggtg cccgtttccg cagtgagttt tctgccatgg gtgccgtggg
     9181 gtttgctgaa gatctggcag gtaaaaccgc gtggatggtt cagcagttcg gcgtggaaat
     9241 gagcaccatg caggagctta ttgagggcac caaaaacgcg ggcgcggatt ttggcgtcag
     9301 catggcagag cagtttgcca tactgggcac cctgtcacgg accatgggca ccgaggcatc
     9361 gggcatctat gaggaattcc tgcgcagtgc ccccgctgcc gcacaaaaaa tggggatgag
     9421 ttttgttgat gccaccggga agattttacc catgggtgac atcctcgacc gtctgcaggc
     9481 gaggttcggc aggaacatcg agggtaacgt ggcggcacaa caggcgctgg atgacgcctt
     9541 cggcagtggc gcggacgtga ttaaaaaact ctggggacag caggaaatgc ttaaccggag
     9601 catatcagcg ctggggcgta atgacggcat gaagcgtgcc acggaaatgg cggaagccat
     9661 gactgacccg tgggaccgcc ttgtggcctc cttctacaac atccgggccg ccatcggtaa
     9721 cgccctctac cccattctcg caccactggc tggccgtttt gccgatatcg gcgcgacgtt
     9781 cgtgcgctgg ctgaacatgt tcccgaacat cgcccgctgg ctgggttaca tcacgctgac
     9841 catgctggcg ctgggcgcag ccggtgcggt cacgaacatc gtgctgggtg tgttcagctt
     9901 catcatggaa ggccataagg ccatcataaa gggcgccagg gcagcctgga gtctgtacac
     9961 gctcgcactc acaaaagtac gcagcgtcat cattgccacc agtcttgccg cgcgtctggc
    10021 gggcgtgtcg tttctgacca tgggcgcccc ggtcctcctg gttgttgccg ccatcgccgc
    10081 tctggtggtg gtggccatta agttctggca gcccgtcaaa gcctttattc gcggctttat
    10141 tgatggtttt accggcgcac tggacgccat gagcccggtg actgccgcgt tcggcgacat
    10201 tgcccgggcg ctggccccgg tatggcaggg cataaaaacc ctgttcggtg ccttctccga
    10261 tctgtttgca ccggtacagc tgaccacggg acagatgaac agcgtcaccc gtgccgggga
    10321 aacatgcggt cgcgtggtgg ccgcagcggt aagcctgata tcccccgcgc tgggggtggt
    10381 tatcagcgtg atcagaaacc tgatcgacat cttcaccatc gtcatcagag ggtggcagga
    10441 cgtggtggct gcatttgacc cgaccgcccc ggtcgaatcc ttccggaaaa tggcggggat
    10501 catcggtaac gtcttcgggg acttatggaa cgtcattaaa aactcgttta accggacgtg
    10561 gaactggctt gcggaaaagc tgaacaaaat ccccggtatc agtatcgaca tgaagcccac
    10621 ggagcaggtg aacaccatga cgcggggcgc aacgggcagc accgtacctc cgcagattgc
    10681 agaaaccacc gtcccggcac tggcaggcaa ccgcatcagc gcagagctgc cacccggagg
    10741 catacgcaac cagatacgca cggacacctc aacccgtatc gacaacagcc ggacgttcgg
    10801 caatctgaca atcaacacgc agaacccgat gacgccgggg caactggatg aatggacgga
    10861 gctttacgcg ggatgaacac gccatcgctt tacatcgacc tgcttatcac tgacggcagt
    10921 tttacgctga acagcggaaa cgaaccacag cgatgtgata accgggtcag catcggccag
    10981 gacgtggtgc acagcattat cgaaagcggg atcgccgccc tgcttatcgg cgaacgcagc
    11041 ccgacgctgc gcagcgacat cattacccgg cttatcctcc tggttgagga tgatgaacgg
    11101 ctggttccgg ggacggtgtt cattaacgaa gaggcaccgg gcaggctgca tgtgacggca
    11161 gagacacagg attttggacg cataaacacc agggtgaact atgaaaggga aaccggacgt
    11221 tgattttaat caggtattcc gtgaaagcgg tatgccggaa acagaagaag agatcgccac
    11281 cctctttcat gaggatgtac agaaaaccgg actcatcacc aacacctcgc ccatgagtcc
    11341 cttctggcgg ctcattctga ccattgtcat acgacccgtt cagtggctgc gtgatgcgct
    11401 ggttgacgtt gtgctgcccg gtatgtttgt cgccacggct gaccggtcac ttcttctgct
    11461 tcatgcgcac gacgtcaaca tcaccccaaa accggccagt aaggccagtg gcgtgatctg
    11521 gtttgccaaa accagggcaa atgatgacgt cctaatcccc gaaggcacca tcgtccagac
    11581 cgccagaatt aacggcgtga tatacagcat ggccgtcacg gaagaaacgc ttatcagcag
    11641 cggtaaaagc ggcgcatccg ttcccgtgga agccaccacc gaaggcagcg ggcacaatct
    11701 cgcgccgggg tattttcgta tccttcccgt tgccattccc ggcattgcat ccgtggtgaa
    11761 ccgggaggac tggctgaccg taccgggtgc cgatgaggaa agcgacgacg aactgcgcga
    11821 cagaatacgc aaccagttta acctcgtgag cagttaccac acggacgccg tctaccgggg
    11881 acttatcgcc agcgtggcgg gcctgagcat cgaccggatc tactttcagc atgacgcccc
    11941 gagaggaccg ggaacggcca acgcctacct gttactggac agcggcgagg cctctgcccc
    12001 tttcattgaa gcagtcaacc gctacatcaa cgaagagggg caccacggac acggggatga
    12061 tttgcagtgt ttcgccatgc cggagacgcg tcaccgtctg gacgtcaccg catacatcag
    12121 aagttccacc accctgccac cggacggcgc ggaagggctg aaacaggata tcggcgacct
    12181 gatacgctgc gctttccggg agaacgcccg ctttaatgtg aaaaaagtct ggccctattc
    12241 gcgcttttcg ttttccacgc tgggcaagga aattcatcag accttcccgg tggtggcttc
    12301 ggttcagttt tcactggaag acatcatcag cgatctggcc gtgccacggc tcgaatccct
    12361 gacagtggag gtggtcattg aatgactttt tgcagcggct tcgctcgctg aaactgccct
    12421 catggatgga caggggagaa cccgacaaac tgctgcgggc cgcccgggcg ttctggtccc
    12481 gcgtatggga atggatcacc tggcccgtgc gccagtttga ccccatgaca tgtgcagagc
    12541 ctgtacttga tttgctggca tgggagcggg acatcacccg ctttaaaggg gaacccacgg
    12601 agatctaccg ccgccgcatc agttacgcct tcatcaatgc acaggaggct ggcgaggtgg
    12661 cgggctttat cgccattttt gaacggctgg gcatcggcta cgtggagtta ctcgagcgcc
    12721 agcccggcgt ggactgggat gtgatcctgg tgcgggtgac agacagccag atagccgaca
    12781 acagcgatct tcttctggag atcatccgca aatatggcag gacatgccgc cgttaccgtt
    12841 ttgaagtgga aaccctcacc cccttcatca tccattatgg cgcatacgaa ggggagtata
    12901 tctgctggac ggccagggca gagtgaatga ctgaaggagt atgactttat ggcgaaatct
    12961 gtaattacca gcgcctttat ccggctcaag gcacagcagg cggcggaagg cacccccgtt
    13021 gtgctggatg agtttatttt tgcaaacgtc ccggacctca acccggaaac gccggtatcc
    13081 cccgacgaaa ccctgccgcc tgaagatcag attgttcacc gccaggccgt tggtaaaacc
    13141 ggcgtggtga atgagaatgc cgtggtttat tcggtcaccc tgggggcaga gctgggtgat
    13201 tttgaattta actgggtggg actggtcagc aaagcaacca gcacgctggc catgattgtt
    13261 cacgccccca cacagaaaaa aatcagaacc cacaacggta acgaaggtaa tgtgctggtc
    13321 cgttccatgc tcatggagta cagcggtgcg aaggaagcca caggtatcac caccccggcg
    13381 cagacctggc agatagactt cacggcacgc ctggcagcca tggacgaacg ccagcgccgg
    13441 gaaaacgtcg atatctacgg gaaggcaact ttttttgatg acgggtggct ggttaagaaa
    13501 ggcgaaacct ggacagtcaa caaaggggtg ggttacgtgg aggggctacg cgcagagctg
    13561 accgaagaca caccaatggt cacgcccacc tccccgtccc gtatctggct gaatgtgtgg
    13621 tggaccggta cgctcaccag tgaatggacg gtggagcacg aaatcatacc agccgacacg
    13681 gacgttacgg aggagcgttt acggaaaaca tatcccaggg acggcattca gcaatatttt
    13741 tatgccatcg ctgaaattga tgaagacggc aacgtgactg acctgcgccc cagaggaacc
    13801 ccggaagagc gccgcagtct ggacgccctg aaaaaacacg agaaatcacg caaccacccg
    13861 gacgcaacgc tgaaagaaaa gggcttcaca cagttaagca gcgccaccac cagtacatca
    13921 gagacacagg cagccacgcc aaaggctata aagatcgcca tggacaacgc cgatggtcgg
    13981 cttgcgaaaa accgcaatgg tgaggacatc cccaacaaag agctgttcct gatcaacgtg
    14041 ggcgcaccac gcgtttatgc aaaagacggg tacgtgggtg aagcagggca acgctggaca
    14101 acagaacaat tcgtggaatg gctgaatgag cgtggcgcct tcagaccagg actctggata
    14161 ttcaaagcca atatttcact ttcccggcac agcgtgctca ctgatacggg ctatggtgat
    14221 atctgccttg gaggctgcct tatcgaagta tcaggcgaca tcaacggggg gatcatcagg
    14281 atcacaacag gtctcgaaag cccaaaccct gccgacatgt acctccgcac acagttcacc
    14341 ggggttgttt taaataaagg gaaaagcacc atctggtacc gcacttacac ggacaaaaac
    14401 aaacccacac cacaggacat cggcgcactg cctgcaagtg agttgttacc cgcaggcgtt
    14461 cccctgccgt ggccgtcaga caccccgcct gccggttacg ccatcatggt ggggcagaca
    14521 ttcgataaag cacgatatcc cctgctggca aaagcctatc cggatggtcg tatcccggat
    14581 atgcgtggct ggacaatcaa gggaaaacct gccagcggtc gtgcggtatt gtcacaggaa
    14641 caggacggca tcaaatccca cacccacggg gggagtgtct caaatactga ccttggcacg
    14701 aaaacgacct catcatttga ctacggaacc aaaacaacct ctacaaacgg acaacatgaa
    14761 cacagcgtcc ccctgagaag acaagatggc ggaggcataa actttgactg gctggatggt
    14821 tcatcgtccg gttctcatat tggtaatgga tacgttcctg ccgcaggtga ccaccaacat
    14881 accgtaccca ttggcgcaca cacccacacg ctcgcactgg gttcacatgg tcacggcctg
    14941 accattaacc ccaccggtaa tgccgaaaac accgttaaaa atattgcatt taactacatc
    15001 gtgaggcttg cataatgacc tttaaaatga gcgaacaccc acagacaatt aaaatctaca
    15061 acctgtcagc cggaacaaat gaatttatcg gcgaaggcga tgcctggatc cctccgcata
    15121 ccggactgcc tgcgcactca acggacattg ccccgcctga cattcctgaa ggacacgtgg
    15181 ctatctttga tgaggatgaa aacacctggc agctcatgga agatcaccgg ggtaaaaccg
    15241 tctggcgtct ggagtcggga agcgccttac tgattactga gtcgggcccg ttaccggaaa
    15301 acgtgaccat aataaaaccg gacggggagt tctgccgctg ggacgggtca tcatgggcta
    15361 aggatgaagc ggcagaacag gcttaccggg tgagcgaggc ggagaataaa aaacgggaat
    15421 taatacagga agcaacaaat acaataaaca tccttcagga tgccgtggat ttaaatatgg
    15481 cgaccgacga tgaaagcgcc ctgcttcccc tgtggaaaaa ataccgggta ttactgagcc
    15541 ggacagacac gggcaacgcg ccggatattg tctggcctga atccccttcg ccggatcatc
    15601 acgcaggctg accggaggca ccatgacgtg gacaagaaaa acaatcagcg tcagcgctga
    15661 catgcccccg ctgagctgtt ccgtcatccc cgtcacaccg tggacttacg ggctgggacg
    15721 atatgaagaa tcgggggtat atttaagccc tccgaacgcc ataaactggc tggccgggaa
    15781 aatggccgga agccgtgcat ccggcgacgt gaccattatc atgattgccg aaaacaccca
    15841 cgatcttttt atacagtcaa tggcggcact gaccgccgtt ctgcccgtgc cggtattcac
    15901 gcaggcacag cgcatggcac aggctgccgc cagtctgagc actgacaaaa tgcaaatccc
    15961 gatcaccacc gacaccctgc ccgcacctgt gcatctttcc gttgccacaa tgcgcagcgc
    16021 agtcagtgcc gcccgtgcag cacaggccag gcagctggca gcacaacgcc cggatgccgc
    16081 agggcttcgc cgccagataa gccgctttat cagcagacgg gctggcgcac ttgccggact
    16141 ggaagagggg atcagaacac tggcagaaca gaaggcgcag gcctgggtat ttcagtaccg
    16201 ggggtatcac cgcgccgcag cggcggccat ggtgaaggat atcccggcac cgaccgccgt
    16261 acataccgtt gccgcactcc tggcggcaga ctcattaact gaactgggga aaatgatcca
    16321 tgagccagac cgcactgctc gcccttgatg gtgagggtat tttcatgcag aacatggtga
    16381 tttcgccctc gatgcagttc caggaaaagg accagtccgg ccagacatcg agcaccgcga
    16441 acgccgagca gggcatcaag gccaaagagc ttcgcgtaac cgggatgatc acctttgaca
    16501 atgagcgggc ccttcagcgc attttccagc tggcctctgc caccaccggg gacggtgccc
    16561 tgaaagttta ccgtatcgca aacgccaccg ctgccgccat taactttcgt gaagggacat
    16621 tttccgggca gatagacgca cagcagcaga ccgaccgcct cgcctggcag gtcagcttca
    16681 cattacgcga aagacgaagc gtaccggaaa aacgccaggc acgcgccacc cccgccagca
    16741 gccgtaaaca gacgccgcag acgcccggcg caaaaggcgg gaccgtggcc gacgaaacac
    16801 cggagaaaat gacctggttc gaagaaaagg tcctcaaacc cgtcaatgat gcgctggagt
    16861 aaccatgaac cccactaaac gtctgttact gggtaacgac accattcagc tggtcagttg
    16921 ttccgtcatg cttgagctga acgcgtgcgg acgcggattt atcacagcca gaacagagca
    16981 gaactacacc gggcgccccg ttcgtctcga tatcggatac ggaaacgacc tggtgcgctg
    17041 gtttaccgga tacgttgaac gcagccagcc cgctgaaaac ggctcggtgc ggcttctgat
    17101 tcgtgaaatg gcggggatcc ttgatcaccc cttcccctgt tcatttcagc acccgacaat
    17161 gcgcacggtc acacagtggc tgagttagac cagcgggctg gaaatcatcc tgccggacaa
    17221 cgcggcttac actgacagac ccgttccgca tttcacacac agcggcacgg gctacgaact
    17281 gctggccagt ctggggcgta ttttctccgt accggattac atctggcatc cgttaccgga
    17341 cggcggcatt atgaccgctg ctgccgcgca ggggatgttt tccggtcgcc ccgtcaccat
    17401 cccgcacgag ttcagccagg catcaacggg cgggcacagt atgaccctcc cgatgattca
    17461 gacgctgcga ccgggtgtgg aggtgaacgg tcagcgcctg acgatggtca tgctggaaga
    17521 tgacaacatg accatcacgt ggacaccacg caataaagcc accggcgccc ccctccagaa
    17581 gtcccctttc cgtcgccagg ctgaaaaggc cttccctgaa ctggcatcag ggcttcacct
    17641 gcccaaactg gcacgtgttg aggcaccgac cgaagccgtc agcgcgggca atattgccga
    17701 cccgttcaga ccccgctacg cggtgaatct ccagctgctt gacgagaacg gcaacccggc
    17761 agcagatacg cccgtctata acgccgttcc cctccccgtt cccatggcgg gcagtgagtc
    17821 cggcatgttc cagttcccgc cacccggaac cctggtggaa gtgggatttg tggaaggccg
    17881 ggcggataag cccttcatcc gccagataat ggcagagggc cacaatctgc ccgacgtgaa
    17941 gccgggcgag cagttgcagc aacagcgcga cggcgtatcg cagcgcgtga cggtcgccgg
    18001 agactgggag cgccagacag accagcggat acaggagaca tcacgcgagc gcgtggtgag
    18061 cgcagacgag gaaaagcgga gcctgaccag ccgggagacc accatacagg caaccgacac
    18121 aaccaccgtc ctgggcgtat ccacgcttca ggctggcgcg gtcacccata tcactgaaga
    18181 gaactacagc atcagcacgg gcgggcacat gatggtggtt gccggtacct gtgagcgcga
    18241 cgtggaaggc gacacccagg acaccgtggg cggcagcctt accgaaagga ttaagggcgt
    18301 ccgtcacagt attgcgctgg cgcagaagct ggttggcgat acggtgaagc tgggaaacga
    18361 gggcattaat gtactgacca tgctgacgga ccttgccgat gtggtggaag agctggcaga
    18421 tattacggca tcacataccc accccaaaac gggcacgtct ccgcaggccg cacagttcag
    18481 ccaggtcgct caggagtgcc gccagcttaa aaataaatat tcaccaataa tcgagtaatt
    18541 attcacttta acaggccgcg caatgtggcc ttttttattc ccccgacaat aaaaaatatt
    18601 catcaccgga catcacccgc cacgaaaaaa ccgtccgcag cagaaaaaaa gatggacgaa
    18661 acgccccacg taatccccac gtaaaacgtc gcgtaacgcc acgtaacgaa gaaataatcc
    18721 cgccagcccg attcgatccc ctgacatcag aaaacgattt ctgcgcccca aaatcgccgc
    18781 a
//
LOCUS       DAZGTJ010000091        18481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000091 DAZGTJ010000000
VERSION     DAZGTJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.091"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..75
                     /locus_tag="ACHK8E_003971"
     CDS             <1..75
                     /locus_tag="ACHK8E_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346176.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            336..677
                     /gene="raiA"
                     /locus_tag="ACHK8E_003972"
     CDS             336..677
                     /gene="raiA"
                     /locus_tag="ACHK8E_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP0346177.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(1338..2009)
                     /locus_tag="ACHK8E_003973"
     CDS             complement(1338..2009)
                     /locus_tag="ACHK8E_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0346178.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(2051..3778)
                     /locus_tag="ACHK8E_003974"
     CDS             complement(2051..3778)
                     /locus_tag="ACHK8E_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0346179.1"
                     /translation="MTVRISGVLKDGTGKAVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGETGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAAT
                     PKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVA
                     WLKEKGAFDQPYWMMKASLPAVLNKVITDVGPGKLNLGGCVIEVMGTYNAAIVRVTIG
                     EYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDT
                     GSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGA
                     TGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQTYAPQT
                     DSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSG
                     ASGFEYFPL"
     gene            complement(3921..4061)
                     /locus_tag="ACHK8E_003975"
     CDS             complement(3921..4061)
                     /locus_tag="ACHK8E_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0346180.1"
                     /translation="MSQKPLKTTVICTTVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(4235..4825)
                     /locus_tag="ACHK8E_003976"
     CDS             complement(4235..4825)
                     /locus_tag="ACHK8E_003976"
                     /inference="COORDINATES: protein motif:HMM:NF025659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0346181.1"
                     /translation="MPEIVTEIRHARYDESGNIHAEMKLPSGECWMDIVVMPHDPDVI
                     WRELFAAIAGGQYGAVTPWRESQDMLEKEKEKACDRINIWRNEEENKEIVFEWQGRRW
                     DAGKRSLSRLEPVLAVTEHLPERFFWTDADNHDVAVDADELKALGVAMKQAMVAHGFR
                     IHERQRQMKQEIAGMTRYRDVLCYQPGWGSEEPVFT"
     gene            complement(5321..8389)
                     /locus_tag="ACHK8E_003977"
                     /pseudo
     CDS             complement(5321..8389)
                     /locus_tag="ACHK8E_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            complement(8811..9392)
                     /locus_tag="ACHK8E_003978"
     CDS             complement(8811..9392)
                     /locus_tag="ACHK8E_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0346182.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATMALWGAALSAGGL
                     TATTMLFSLGVSMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            complement(9389..10132)
                     /locus_tag="ACHK8E_003979"
     CDS             complement(9389..10132)
                     /locus_tag="ACHK8E_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0346183.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRITPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            complement(10143..10841)
                     /locus_tag="ACHK8E_003980"
     CDS             complement(10143..10841)
                     /locus_tag="ACHK8E_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0346184.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(10841..11182)
                     /locus_tag="ACHK8E_003981"
     CDS             complement(10841..11182)
                     /locus_tag="ACHK8E_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0346185.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVMTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(11175..14417)
                     /locus_tag="ACHK8E_003982"
     CDS             complement(11175..14417)
                     /locus_tag="ACHK8E_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0346186.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESAHQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSQLVMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRGGKSETEKAEASFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREETLKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGRMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(14481..14894)
                     /locus_tag="ACHK8E_003983"
     CDS             complement(14481..14894)
                     /locus_tag="ACHK8E_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346187.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(14958..15167)
                     /locus_tag="ACHK8E_003984"
     CDS             complement(14958..15167)
                     /locus_tag="ACHK8E_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP0346188.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(15263..15637)
                     /locus_tag="ACHK8E_003985"
     CDS             complement(15263..15637)
                     /locus_tag="ACHK8E_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP0346189.1"
                     /translation="MKKELKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(15652..16368)
                     /locus_tag="ACHK8E_003986"
     CDS             complement(15652..16368)
                     /locus_tag="ACHK8E_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0346190.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(16434..16778)
                     /locus_tag="ACHK8E_003987"
     CDS             complement(16434..16778)
                     /locus_tag="ACHK8E_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP0346191.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(16775..17221)
                     /locus_tag="ACHK8E_003988"
     CDS             complement(16775..17221)
                     /locus_tag="ACHK8E_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0346192.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(17218..17568)
                     /locus_tag="ACHK8E_003989"
     CDS             complement(17218..17568)
                     /locus_tag="ACHK8E_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0346193.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(17578..17904)
                     /locus_tag="ACHK8E_003990"
     CDS             complement(17578..17904)
                     /locus_tag="ACHK8E_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0346194.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(17901..>18481)
                     /locus_tag="ACHK8E_003991"
     CDS             complement(17901..>18481)
                     /locus_tag="ACHK8E_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0346195.1"
                     /translation="TPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLDAVSA
                     LPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVA
                     DIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLW
                     HCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
BASE COUNT         3851 a         5948 c         4273 g         4409 t
ORIGIN      
        1 gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc
       61 cagtcagttg cttaactgat cggcattaca gcccttgagc tgccgttttt ttattctgtt
      121 agttgtgaaa ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag
      181 atattcctca agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac
      241 aaaaagtgac aaaattatga gatttccatc acacattttg acatcaggaa cggtatgctg
      301 aattcaccaa gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac
      361 aaatggaaat tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa
      421 aatggcaaac acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg
      481 ttgctgacgc cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag
      541 atatgtacac cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc
      601 agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag
      661 aagttgaaga agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggtgcttt
      721 aaaactgaca tcaaccaatt gaaagttaaa ggaattaaac tttcaatgtc cataaaacgt
      781 ccatttttgt agccctaaag ccccttatca ggggcttatt ttattcgtga taaaaatcgt
      841 catgaccatc ccttcctctg aagcatacaa ccgggcccgt gcaaaatacg gacttccgga
      901 aatctgaaaa attactctta tcagagaaga gcgtcagaaa cacgatcggc atcactgttt
      961 ctgcaaactt ccctgtggca gtctttattc gccatataac gtagcactaa aggtcttcat
     1021 taatttttcc attgtagtta ctccttcctt gtaccggggg ttggtcatgc ccccgttttt
     1081 tattcccctt gttcccttaa tcgtactgcc aggcgccaca acacacatca actgtggctg
     1141 gtatggaaac accaaaaact ctcacttccg gaaatctgaa aaattatcca tatcaacagg
     1201 atgcgccgga aacatgattt ccatcacatc caggcctgca ctttccgtga cagtatttat
     1261 tcgcatagta aatacttcac aagtgtataa ttgactcagt tcatggggga ggtgatggtc
     1321 ccccatcttt ttgtctgtta ttcctgtgcc cagccaaccc tgtatgccag tatctcatcc
     1381 gcactggtca gcttttccag ctctttcttc atggtgcgct gccggacgtg gatttccatc
     1441 cctttggtga acatcgcctg ctctgccgct tcactcagcg ctataagctc ttctgctgtc
     1501 acctccacat cattgttttc cgcatccgtc cagaaaaacg cctccggcag tttccccgat
     1561 ttcgctgccg ccaccgacgg ctcaagacgt gtctgcgttg atttcccgta gtcccattta
     1621 cgcccattgt gctcaaacgt gtagttcgcc gcttccatcg cattacgcca ggcgttaatt
     1681 tcatcgccct tcatccctcg cgctttctcc gcggtcagca ggtccgtgat tttctccccg
     1741 tcaaaacccc agcgccccga gatgtcagct ttgcggtttt cttccgtatc cgccacctcc
     1801 accacactct ggcccactgg ccacagaact gatgccaccc tgccgatgcc ggtaatgact
     1861 ccggcactgt tataaaccac cttcagcgtc tctgctgaaa ataacgactg acactcatac
     1921 cagtcctgac catcctctga tttcaggtac atcgcacccg gcacatccgg attttccggc
     1981 gtgtattgtc tgaaattctt tatgtgcatc tgtaagtcct cccggttaca tgtctgaaag
     2041 atgatgcgct ttataatgga aaatactcaa accccgaagc tcctgacgac acatactcac
     2101 ccacgctgac tgtacgccac tgagaaccat caaatgtcac ctgaagcggc ctgtaagata
     2161 ccctgtcaat aagggttttg tttccgtcgt tgtaaccgcc aatccccgta atcattgcac
     2221 caatatccaa atccactgcc catgtagtgc tgtctgtttg cggcgcataa gtctgtttat
     2281 tacccagcct gatacccaca acccggctct tcccgtctga tgccccgggc ggccccatcg
     2341 gcccggtttc tccacgctca cctctgggac cggggtcgcc tttctcccct ttcggtccct
     2401 gcgctccccg cggaccagcg gggcctgttg ctcccgtttc acctttcgga ccctgtgctc
     2461 cggggtcgcc tttctctcct ttcggaccgg gaacaccacc tcctgctgca gcctcttctg
     2521 cctttgtttt cgcttcattt gccacatcca ttgccgcctt cacggccttc ggcgtggctg
     2581 cctttgtttc gtcattactg ccagtatcac tgcttaactg caccacacct ttctgcgtcg
     2641 tgctggcatc ccgctggctg accgtatccg gtttgttttt tgtgctgtaa tccacccgcc
     2701 agtggaaata ctgtgtgtcg ccgtaaaccg tacaggtaca gaccgtgcca ttaataaacc
     2761 ccgttgcacc gtattcgcca atggtgaccc ggactatggc tgcgttatac gtccccatca
     2821 cctcaatgac acagccaccc agattgagtt ttcccggtcc gacatccgtg atgactttat
     2881 tgagtaccgc aggcagtgat gccttcatca tccagtaagg ctggtcaaat gccccttttt
     2941 ctttcagcca ggcgacaaac tcgctcgtgg tccattcacc ggcccccgta tgaatgtccc
     3001 gcccgtatac tctggctgct cccacggtgt tcagaaactt caccttatcc gggatatcgt
     3061 caccgttttt cgctttctcc aggcatccgt ccgctttgtc cattgccgct ttcaccgctt
     3121 tcggggtggc tgccttcgtt tcatcatcac tgtccgttgc gctgcttaac tgcacaattc
     3181 ccttctgtgc cgtcgtcgca tccggtcctc ccggctcgcc tttttcgccc ttctctcccc
     3241 gttcaccttt cacaccctga agcccctgtg gtcctgtttc accacgctca ccctttggcc
     3301 cacgctcgcc ggtatcgcct tttggcccgg gaataccctg cggcccggtg tcccccttat
     3361 cccccttcgg tccccgcgca ttctctgccc gtttcttcgc ttcctctgca ctcgccgccg
     3421 acgcttccgc acgtttcagg atttccgctg ccaccgcttc cagctctgca agggctttcg
     3481 ggtaatactg tgcgtcttcc aggtccatca gaaatttatt cagcgttccc ggtgcagaat
     3541 ccgccttcac cagaatgtca cccacatatg acggcgcgta cccttccgtg ttcagcgtca
     3601 cccggtacaa ccccggctca acatccatac tgtaactgcc ggtttccccc ggctgaccat
     3661 acgccaccgt ggtgacaatc accgtctccg ttgtgcggcg cgctttcagc tctatcgtgc
     3721 atcccggtac cgcttttccc gtaccatcct tcagcacacc cgatattctg actgtcatgg
     3781 atttccccca taaaaaaacc gcagtaccgg tttcccggcc tgcggtaaaa tttgtggttt
     3841 gttggtgtta aaacggtgcc atccggctga ccaccctcag caaccggtcg gcgggggata
     3901 ttctcccccg ccacggtttc ttactgctta cactgtaaga acgccgcaat ctccgcgccc
     3961 gctatccgga accggaactc gcacagtgaa gtgtgggtga tccagataat gagcactacc
     4021 gtggtacaaa tcacggtggt ttttaacggt ttttgcgaca taacgcttgc cccttttacg
     4081 gagaggcgct aacctttcac ttgcttagga tgaatggtca ggcctcgggt taaacataaa
     4141 tgttttgtcc ggggcctttt cacatccggc cttcaggtgt tccctccggc catcagccaa
     4201 aggcacccgc gcataatgta cggtttttat tctgttacgt aaataccggc tcctcactcc
     4261 cccagccggg ctgatagcac agtacatcac ggtatcgcgt catccccgca atctcctgtt
     4321 tcatctgtcg ctgtctttcg tggatcctga aaccgtgcgc caccatcgcc tgtttcatgg
     4381 caacacccag cgccttcagt tcatcagcgt ctaccgccac atcatgatta tccgcatctg
     4441 tccagaaaaa acgttccggt aaatgttctg tcactgcaag cacgggctcc agacggctca
     4501 gtgagcgctt acctgcatcc cagcgtcgcc cctgccattc aaacacaatt tctttatttt
     4561 cttcctcatt tctccagatg ttaatcctgt cgcatgcctt ctctttttcc ttttccagca
     4621 tatcctgact ttcacgccac ggtgtcaccg ctccatactg accaccggca atggcggcaa
     4681 acagttcacg ccatatcaca tcgggatcat gcggcatcac aacgatatcc atccagcact
     4741 cccccgacgg taacttcatt tcagcatgaa tgtttccgct ttcgtcatac ctcgcatgac
     4801 ggatttctgt aacgatctcc ggcacaatcc ccccttaaag cacatgaacc ttctcacttc
     4861 ctgcccggaa taacgtgcca ccgttacata gaacgctgat cgtcacgcga cagttataat
     4921 tcagggtgtt atgaaaggcg cgcacgtaac gataccggac ataaatccgg ttgtggtttc
     4981 ccctcccctt tgccggaagc gtgaatctgt aaatccagcc ataattttcc cacaccttat
     5041 gcttcaaatt cccgggcccc cattctcctg acgaccagac agtggcaatt ccaccatctg
     5101 cactatcagt gccgacactg acttcccacc aggcagaaac aagaccagga ttgccctcag
     5161 ctctgaaggc tatcttcatg ttaacatcaa aaaaacgatc gaagttttcc ccgtcaacat
     5221 tcagaaaagg gatccattcg ttatctctct cctttacacc atcccgtgag ctgacaccac
     5281 tgaacacaac tttattgatt tcaaacaggg agcggtaata aacatcgcca agaatattac
     5341 cggcatccac tgttccttta acgacgcagt tttcttcaat ggtcacgtta ttcagcgtgc
     5401 ccgagttcgc actgatatgt ccgctgatgt ccgcattgcg ggccgtcagc ctgccctccg
     5461 gcgtcaggga gaacgtcggg ggattgccgg acgaggtgat gctcactgca aacaaccgct
     5521 tcaggaacac atcgttcatg aacagctgat tcccctgcgc cacaaacagc ggcgtggtgt
     5581 tgccgttctc cgggttaatc atcgcgatac ggtccgcctg cagcagtata ttgctcaggg
     5641 gctggccatc agcatcctca atccccgcac ctataccggc aacatacgga atgccgtttt
     5701 tcgttttctg caccttcagc atgtacagcg cagccaggtc gttatttgtg tctgtctgca
     5761 cccgctgtat ctgctgtatg gtggcgctct ggtcttccag ggttttactg accgtctgcg
     5821 tgatttcatt gcgggtttct gtgatggtgg tcttcatctc cgccatctca tcatcaagct
     5881 ggctgttatc aatcagctcc cacatcccct gtgccagatg tgtcttcccg atttctcctc
     5941 tgaaaaagtc cagataccct tctgcatcat tgctggcccg gccactggct tccacaaacg
     6001 cagatttccc caccaggttg acgctgcgca cataaaacca gaaatccttc cccggcttaa
     6061 tgtgcgggcc ggagacactc cactggctgc ctgtccccag ataacgggca gaggtttcca
     6121 cctgagatgt gtctgcgatt tttgcctccg agaaccagaa ctcaaactgt accgtcgggt
     6181 catacaccgc cagcaccggt actgctgtta tctgataata ccccggcgtc agctcaatgc
     6241 tggccggtgc tgcaggcgca ttaatccgga acgcggtggt ggcaggttcg ccctgctggc
     6301 cgtagctgtt tatcgccctg accgtcaggg tgtattcccc cagaggcagg ccactgaaac
     6361 ggtgctccgt gtcggcagtg atggcggtgg tcaccaggcg gctgttttca ccactgccac
     6421 tggtcaggcg cagactgaag cgcacgccct tcaccacccg cggcgtgtcc catttcgcca
     6481 gcgccagata ctggccgtct gaggcactca cctccaccgt gaggtgctgc acagccggcg
     6541 ggatgacgct gttcagtgaa ccggacatcg gctcaaagcg ggccccgtta tccacgatgg
     6601 cttctttttc cggtacgtgc tgcactgccg tgatggcaaa ggtgccgtcc gtgttttccc
     6661 ggatggaaac acagcggaac aggcgacgac gcagtgacgg cagggagagt ccccacacac
     6721 cgtatgtctc cacgccatcc ggcaggacgc tgacctgtat ccggtccggg gcggggtgtg
     6781 cggtgatgtc cacgcgcacc ggcttaccgc tgccgttaat caggttcacc gtcgatgtcc
     6841 ctgtctccgg cagtatcacc tcacggtcca gcgtcagggt acggctggcg gcatcgatgg
     6901 acaggatgcg tccgccggtc agggtcccgg cgtagtcgtt atcacagatt tcaatgatgt
     6961 caccgggcgt gtgccgcagc ccctgtgacc cgagcgtgaa atccaccgtc tgcgtttcca
     7021 gcagttctgt ctttatcacc cacagtccgg cacggtgggc ctgaccgcgg ctggtacagc
     7081 caaacgcatc catcttcagc agattgcgcc cgtagcgcag gatggcgtcc gggtcttcca
     7141 ccagttccgt ggaagtctgc cagccgttct gcgggtcggt gtaattcacc tccaccgccg
     7201 tgtgccggtc cttcagggca ctgaagctgt agcggaaccc cacgccgtta tcatccacca
     7261 ccacatcgct gttggtgtac ggccacacca catccgacgg acggtcctga acgaacgtca
     7321 gcgtctggcc gttccatacc ggcatacagc gcatcgccga gcagaaatcc cccagaacat
     7381 cccagacctt acgctgctgt gacaggtacg cattgaaggt catccgcggc tctgttcccc
     7441 cgaaaccatc cgggaccgtc tggtcacagt actgcccgat ggcatacagt gcccacttgt
     7501 ccacatccgc ggcccccagg cgttttccca tcccgtagcg cgggtgggtc agcatgtccc
     7561 acaggcacca ggccgggtta ttgctgtatg ccggtttcag actgccgtcc cagataccgc
     7621 tgtaggtgcg tttttccggg tcatagtttg acggcacctg aatgatgcga ccacggatat
     7681 ggtagttcac cgtcagctgc tggccaccaa actgctccgc atccacctgc agccccacaa
     7741 tagccgtgtt cgggtagcac tgtttcacat cgatgatttc ggtgtatgac gaccacagcg
     7801 ttctgttctg cagctggtcc gtggtgctgt ccgccgtctc cctgaccatc cggatgttaa
     7861 aggggcgctc agggagatta ttcagaatca ccgacgtcag gtactgcgag gtggtcttgc
     7921 cgttaatggt gacatccttc tccgtcaccc agtgcccgtt acgctcaagc tgaatcagca
     7981 ggcggacaga agagggatta cggtcaccct ttgaggtggt ctccaccagt gactgcaccc
     8041 cgaaggtgac ccgcagacgg tcaatgttcg ctgacgtgat ggtgcgcgtc accggctttg
     8101 ccttcgtcac ctccacgccc agtgcggttt ctgccccgga ggactcaaag ccttccggcg
     8161 gtgtctgctc ctgctccccg gcacgccaga cggcggtcac accgtgtatc acgggattac
     8221 cgtccgtgtc cgtcagcggg gttttgttca ccagaatact ctgcagtccc ttcaccggac
     8281 cttccaccgg tccctcacca atggcatcaa tcacgctcat catctgcgtg gatttgagat
     8341 tgtccttcgc ctcacgcggt gtgtgcccct tgccgccccc tttacccact ctgtccccct
     8401 ctcctgtctg atgtctgaat ctgtttatgc cagaaaacga caggcacccc ggagggtgcc
     8461 tgtgtcatga cggaataaaa tttctgaaat tcttcacatt tccggcaaat tcccgtggag
     8521 gaaaccttgc cggatgggta gattacctgc ggtcaataaa agcaattaat cgcaactcct
     8581 taaggtgact attcgttcct cccgttaata tccaggtgta ttgcgggagc gttttttatt
     8641 tcagctccgc aacagaaaat ttctgaaatt cttcacactt ccggcaattg cctgtagccg
     8701 caataatgac gctgcgttac tgttcttctg cccaaaaaaa taactccata acgttaatct
     8761 tcatcgttat ttcccgcagc tccgctaact ctgcgggatt ttttttattt ttatccccgc
     8821 ccgataacca ccactttccc gtctccgccc tcatcacggg tgctgatgtc ctgggatatc
     8881 cgtcgtgaac caaccagcat ttcaccataa ggcaccggca tagggttacc ctgggcaatc
     8941 atgttgtcca gtgacgaaaa atacgtgttc tgtttaccgt tatccgtact tttgtactcc
     9001 ggcgtctttg ccttcggggc cagcatctgg gccacaccac ccagtatcat gctgacaccc
     9061 agtgagaaca gcatcgtggt ggcagtcagc cctccggcac tcagggctgc gccccacaac
     9121 gccatcgttg cgccgccggt gaagaaagag cccacgatgg ctgctacccc cagcaccacc
     9181 tgaaaaacac catttccccc ggctccggcc agtcgcggta caatgtggat gaccgctccc
     9241 tcacccagtt gttcgtgaag acgggcgtac actgcctccg gtgcggtatc ctcaccggca
     9301 atacgtatct ggtaccagcc ttcgttcatc tgaccgcgga atcccggcac ctgcagcgac
     9361 agggcacgga tggcctccgc tgccgtgttc acatacaggc tgatgcggcg accaaatcgt
     9421 tgtaaatccc cgtgaaggca gatgcggacc agtggcggtg acgccagaca gaatgcgttc
     9481 gtcgttgcca tttttcagaa tacctctccc gtttactcag ttgttcaggt atatggtgaa
     9541 gcagttcacc gttgccacag taaatggcgg catgattcgg caccgatgcg ccgaagcagc
     9601 acagcaggat atcgcccgcc tgtgcacagg acggagacac ccggtaaaag ccgttttccg
     9661 ccaggttgtc caggtacagg ttctgaccgt tgcgccacca gtcatcctca cgcacaaaat
     9721 ccggcagcgt tatccccgcc agatggtatg catcccggaa cagggtgtaa cagtccgtca
     9781 caccgtgttc aaagcgccgt ccggtcaggt gcggcacaca gcggaaccgg tgaatttcac
     9841 cccggcagac cagccaccag ggcagggcac tctttatctg cagccgccgg tccgcctcgc
     9901 tcagccaggg cagaccaccg ggatgactgt ggaccagcgc cacaatctcc ccctgcatct
     9961 gtgcctgcag ccagtcttcc ggtgtaatac gaaaatacgc ctccggctct gcggagatat
    10021 tcatgcaggg ctggtaccgt tcgccctccg gggtgcttat cacgaagccg cacgactccg
    10081 ctggcgcaca ccgccgggca tgcaccagaa tcgctgattc agtctgtgtc ataaaacggg
    10141 atttactgcg aaagtttatt gatggaaagg aaaccgccaa aattgaccgc catgccgcgc
    10201 atctcacacc cgcgcatgca tttactgcat ctgtccttcc ggatatccgt ggtggggttg
    10261 tcgaactcat ccgccactgc cggaccgtta tacccgcact catcgccccg gtaatcccac
    10321 atacaggtgt tcgccagcat gatgcgaccg ggaaacagcg caccgtccgt ctccgtcggt
    10381 gtcgccagca caaacgaggc cgtcatggcc gtcagctctg ataactgctc caccacccac
    10441 cggtccgtca gctcctgctc cgggtctgcc tcaggattgc ctgccacaaa attcaccgca
    10501 tccagaaaac gcgcatacac ccggcgacgg accaccgtgg cacccaccag gctctgcaaa
    10561 tcctccgcca tcccggtgac cagcccgaaa agattggaga ccgtcagcga cgggcgggca
    10621 ctgctgccct ttccgttcat ctcaaagccg ctgccctcaa tcgggtacgc ctgatattca
    10681 cgcccctgcc aggtcaccgg ctcccctttt tcattcagct cattgcagaa aaaataccgc
    10741 tcaccgccct gcaccgtcag gtcgatttcc cagagcacca cccgcggtga ctgctctgac
    10801 ttaaccgact cgttcaggct ttcttcgtga atatcctgca tcagttcacc acctgctcta
    10861 tcgtgcaact gaaatcactg taccgggcgt tatccgtaat actccactcc cggcatacca
    10921 ccctgaccgt ccggttatgt ttcgggggct tccacaaaaa agcccggtaa ccgccatgcc
    10981 atgacagaaa cgcggacagt gcctcccgct cagcatccgt tgtcacccga aaaaccacct
    11041 gaaaggtctt cagttgcgca ttcagtcctg ccggacgacg ctgctgataa ccgtcgccaa
    11101 acttcaccgt catcaccgac ggtttctccg tcacctgcat cccttcccgg ggacaccagt
    11161 gaagggtctt aatctcatcc actcagcatt cctccgtcac gacgcatgga taacatcact
    11221 gcctgtaccc tctggtctat cagttgcaca agggtgcccg ccgcttccgg ccctatctgc
    11281 ccgttagcgc cgtcattctg aatggcgatg tggtacaccg gggaatacac cagacccgca
    11341 ctgccgttca tgcggcccac cgcacgcacg ccgagtgaac catccgctgc ccgggtcagg
    11401 ggcattatgg cttcaggtcc ggcctccccc atcagccctg ccccttttgc aaaggcaaag
    11461 tatgtgggcg tgtccacaat actgttactg tatgcactca ggtttgccga ggtataaacg
    11521 ccgccttttg catttgccac cgcgccaccc agccagttgc cgatactgcc gaaaaatcct
    11581 cctgcaccgg acatactgtt tgccgccatc ttaatgccgt tgacaatggc cgcattcata
    11641 aggacttttg atatttcctg caggattgat gcagcccagc tgcgccattc cactttattt
    11701 ccgttcagca tctccgtgat gttattcacc atccctgaga taccgtctgt cgcaagctgt
    11761 gctgcctgtg aagcgtaatc tgatgcgctg tccacccagt tacttagccc ttcctgcagc
    11821 cctttctgcc agtcagcacg ctgcgcatcc gattcggcat aaaaagcctc ctggtcttta
    11881 aggcgttcac tcagatactg cgcattctgc gccagcgcct gtctgtaaaa atcctcactg
    11941 atatcccctg tctgatactg agactgcagg tccgcatcct tctggcggta gccgtcacga
    12001 atctgctgca gctcccgcat ccgttcacgg acccgctcac cctgcccgta ccccagcagc
    12061 tcagcgtcat ttgatgccct ttcgtctgca ttatcatttt tcagggtctc ctcccgggcc
    12121 cgtaactgct cccggatttt cttctggtca atcagggcag cattgcgcag cagctcctgc
    12181 ttctgtatct ccgtcagggt tttcagctct ccctgcgagg tctggtactt cagcttcgcc
    12241 agttctgtat tcttcccggc cagtgccagt tgttccttct gctgcttcag cagacgggaa
    12301 aaactggctt ccgctttttc cgtctcagat tttccgcccc gggatttggg tttattcgcc
    12361 tcattattgc gccaggcttc catcgaatta cggatgtaac gctgcctcgc ctcctgatac
    12421 gtatccccca ccagaccaag gtcatccgcc gcatacccca gccgggcacg ctctttttct
    12481 tcccctttca gccgggacag ggccagttga cgctctgtgt tattcagggc gctctgctgt
    12541 ttatcatcca gagtggcctg tggcagtcgc agcggtacac tcgccagtcc ctgacgctgt
    12601 tgcagcagtt cattacccag ccccagcagg cggttgaatt ccgtatgctg accgttcatg
    12661 accagctgtg actgatacgc tttattctgc tctgccgcct gctgacgaat taacacaaca
    12721 cggcgctctt ccagtccggc aagcacatcc tgaatggact gcgctttttc ctgcatctgt
    12781 gccagacggg actgctcaac agcaaactgc tctgttgcct gagcaagccc ttccgttaca
    12841 gttttcaccg atgtcagatg gtttatcatg aagccgttac cggtcgtcca gcccggatta
    12901 gccagcacat actgataccc ggcgattttt tcctgcaggg atttcacccg gctggcctgc
    12961 tcatcaatca gccggttctg ctctgtcagc gcctcccgtg ttcgcccttc attatctgag
    13021 gcttcaggca gagacattga tggcgtttta tgcgcgattt catcaatcgt cagtgcatac
    13081 tggtgcgcag actccctggc ctgttcctga ttctggtaca gcgtgtacca tgcggcagct
    13141 cccagcatca ccagtccggg tacgcctcca accagtccca gcgcaccgct catcagacgt
    13201 gagcccaccg ccgttgtact gttcagcgca ttctgggcgg cggttctggc agcaatattt
    13261 ctgttcaggc gttcctgtgt gatcgccaga cgggcctctg ccgcaatctg catctcagtc
    13321 ccgcgggctg ccgccacagc ctgctgagcc cggtacacgg ctgcccttgc ccgcgccgtg
    13381 gcaatctgcg ttccccttaa ctgggcctca gccagtgcca cttcattacg tgcagcggtc
    13441 acaagtcctg ccgtggcaga catcgctccg gaagccagat taccaaagta ccgggccacc
    13501 ccgacagcaa ccagcacacc cgcagctgtt gccacggtat caatatttcc ggccacaccg
    13561 ttcagtgcgc cggagagcgt tttcgtcgct ccgctggcct cattcgcgcc gcccacccag
    13621 gccataaagg cgttttccac cttcgtgatc ccgtcagaca ccgtttccgg catggctgca
    13681 tattcatcac gcaatatccc cagctggctg attaacgcgg gaacgacttt atccgccgtc
    13741 agttgaccat cgtccgccat cgccttcaga tccttacggg ccacgcccat gcctgcagcc
    13801 agtgcgcgaa tgatccggtc cccactttca ttgaccgaat taaattcctc gccgcgcaac
    13861 accccctgtg ccagcgcctg gctgaactgg gtgatcaccg aacccgcctc tgccgcactg
    13921 gcaccggaaa ttttcagccc tgtcgaaatg gcctccgtca ccttcagcac atcatcagca
    13981 ctgtaaccat attcacgcat cgaggcagcc gaacgggcaa acagggccgc attatccgaa
    14041 aatgcggtgc ctgtccgctg gctgatgtcc atcagcactt tctgtgatga cgcaaattca
    14101 tccgatgact gcgacgcctg tttcagacgg gcattcacgg agctccattc atccgccagc
    14161 gaaatcaggt gtccggtggc aaaggcaccg gcaaacgcac cggtcattcc gacagccgaa
    14221 gcgcggattt ccgtcaactg gctgttcagc tcagccagag cccggcgctg ctccctggct
    14281 gccgcagcag cctgacgtcc gccattctgc agggtccggt aatattcact gcccatacgg
    14341 gacgcccgct ggatctccga ctggaatgac tgtgaatttg ccgaaatttt gataatcagt
    14401 tcacgtaacg tcgccattca cctttctccg ggcgaaaaaa aacctgccgc agcaggtttt
    14461 attcatcagc agattattga ttaagttttg ggtaccagcc cgggattcag actttccgga
    14521 ctcagtgtat acataccgcc attgaatggg tccacaagca accagccaac aataccacca
    14581 aagacaaagt taccaccaat gtaccatcca ttagccgatg ccttgatcgg cagcgttacg
    14641 ggctgataat tctctttact gaatgtgatc tgataactct ttttcccgaa gtaactgccg
    14701 tcagattttt caagtgttac attctgcggt gttttgcccg aagaaattgt tttccctgac
    14761 tcatctttaa taataaaatc cgcaccagac ggattactgt ttacctgtac agtctgcgtt
    14821 ttatctccga caatggttgc acaccccgtc agaaaaaagc tgacagagaa aatcaacagc
    14881 atgaatttct tcataaaatg gctccgttca gttaattttc cccgaaaata ctgtaataaa
    14941 gatctgcaaa gttaaaatta attttccgaa gcaaataaca gagcatcctc cagagcggcc
    15001 aaaggatctg cctccggctt atcctcatcc tcgccccagc agagcatggc gtccttcagt
    15061 gcaacattca tcccctgtgc cccgaaaacc gctttcacga tctgtgcatt acggatatcc
    15121 ccgcgctcat cacccagcgg ggataccctg tcgaactcca tccacatcat cgcctcgctc
    15181 gcactcaggc tgtggcgcag ttcggataag gtgcgcccca gacggagcgc aagtcgcatc
    15241 agaaagcgaa tttccgggcg ggctactttt ttctggccga ctctgcatca gcgatcagtt
    15301 ccagtgcctg acgcagcaag cgggcatgta ccggaccata gacggccagc acctgctcac
    15361 ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca caggacatcg cagaacagcg
    15421 tcacatccgc ttccaggtta cggcgggttt tcgccaccac cgacagggta tcgtcatcct
    15481 ctccatcacc attgagcacg tcctgccaca gataccaggc ctctgccgaa ggctcccgca
    15541 gcaccacgct gacattaccc cattccggca ccttcaccgt tttatgacga aaccctgaca
    15601 gtctggccag cgccagcgtt ttcagctcct ttttcatgat gacccatccc cttatccggc
    15661 ggctgcgctc actgtcactg tacattcaac agacgtcaca ctctgtgctt tctctgccga
    15721 atcagtcacc acgcaggtat atttccccgc atcagcggac tgcgcacctg gcttactgaa
    15781 ggtgtctgtc gtctgcccgt caaccggctg accatccttc ttccaggcgt atttatacgg
    15841 cggcgttccc ccgttggcac tgactgacat tgtcagcagc gcaccggtat tcacggtaag
    15901 tgtcttatcc agatttttca caaacgccag cggtaccaca aaggacaccg gtttgccttt
    15961 cagacgcagt gagaacgttg cagccaccac gccgttggta ccggatgacc aggtgtgctg
    16021 gcgcacttcc gccaggaatt taaagccctt accggacgga aactgcacct taaacgcata
    16081 caacgcgtca ttgtcataag catcacgcag ggcgttctgg gcctgattca gataaaaatt
    16141 acccgacatg gaaatctcgg acgacgcccc cagaccattg atattctcct gctctgtgga
    16201 gcagagcgtg gtcacatcaa tatcctgttt ctgaccggcg gtgaactgga cttccttgat
    16261 ggtgcagtcc aggcgcagat attccgcctt atccatagtt tcagcagtcg ccggggcaga
    16321 tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa gcagacattc tgcctcctga
    16381 taataaaaaa cccgcacgcg gcggggtatg ggttttgtag aaaaaagaaa aagtcacacc
    16441 gtgacctgaa actccagggt tgcacggtaa cagcggtttt ccggaatata gtcctgcatt
    16501 tcactgacgg atcccggggc cagcagcatt atggcttcac gggcgtcctg acgtatctga
    16561 cgcgcctgcg tcacagtccc ggcataaacg tctatctgca ccgccactga ggactccgcc
    16621 tgcccgccca tcacgtccgc agacaccgat gaaatcaggc tgaaaaccac ccacggaagc
    16681 gccaccgacg gcctgccatc cagcaggggg accacatacg ggtacacctg cccgccggca
    16741 agatgcgcca gatgaggata caaatccgcc tccgtcatcg tctcagtacc tcatcaatgg
    16801 cccggttcat ccgcgcaatc gccacctgcg ctgcctgttc actgcgcaca tcaaacgccg
    16861 ggcgcacaaa cgggtgcggt ggcatattca cggtccccat ttccacaaac cgccagtaga
    16921 aagcattgcg cgggttatcc gccttcatgg tgttatcgct gttaccggtg tccggattaa
    16981 caccacggat atggacaccg gattccatcc cgccatcgcg ggagcgccgg gaaaggacca
    17041 ccacattgcg gcgcagtttt cccctgcgca ccggtgcccg tgacaccact tcttctttca
    17101 gcacattcgc acccgcacgg gttgcctcac gcagcacccg gttattttcc gcaccactca
    17161 gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg gtttcgatca
    17221 tttttcacct cccagccgac agagaatttc cagacgtccg ccggtcgcat ccggcacggg
    17281 cagcccgaca acgttcagga tccggtcacg ccagggacca ctcagcacat gaagtcgtga
    17341 cgctgccgtg atttcccgac cggactgacc gcgcacccag atgcggattt ccgcctgcgc
    17401 catttccgca ccggactgca tccgctcccg gctgctcctg ccacggatat ccgcatgaat
    17461 tttcccgcat gacacccatt cttccgtcat ttctccggca gcattacggg ttaacaccgg
    17521 gttcagaaca cttatcatct gtgtcagacg acctgcagat attgccattc cccctcctca
    17581 taacaccgtc ggacaacgca aatcgtaaat cagcacggaa acagaaaacg gcagctcccc
    17641 ctgaatcagt tcttcccgct ccgcaagatc cggattccgg tacagcatcc cggtcagtcg
    17701 catggcagcc cccttcatcc gggttaatgc ctcgcccggg atcagttcac cgtcctcacg
    17761 aatcacctta tcccggctgc cctgaatgta ggccagcagc acggcggtag cctgacgaac
    17821 cttgtccatc agcatgtcat catccgcgtc atggtcgaca cgcagatgtg ccttgatctc
    17881 ttccagtgtc agtaatgccg tcattttccg cctccagcat cccgcccacg ttttgcagcc
    17941 agggtccagc ctgatgaatg agcttctccg ggtttatcac cggtcatact gttgcagtgc
    18001 cacagcgagc ccccccacgt cactgtatcg ccggggtggt aggtttcacc ggctctgaac
    18061 acaccgcggt agagcatcac cggcagggaa aatgtttttt ccgtacgctg gccactgctc
    18121 tgccggacca ccacagagaa caaccgttca cccgtcatgc tgacgtcaat atccgccacc
    18181 ccgtcaacca ggcattccca tccccgcatc ccgtgcgttt tttcatacgc ccgccagagt
    18241 cctccctggt gtgtggcata cgtgccccgg ggaaaggatt tttgatcgtc aatggcgggg
    18301 agtatttcca gtgccgtggc atcacgcccg tcctgcggag ccggcagggc actcaccgca
    18361 tccagaaccg ccttctgcag aacatcggga tcatagtcac gaccatcacg cggaacagga
    18421 atatggctta ccgcctcctt caccatctgt tcaagcatcg gacgcacatc atccggggtg
    18481 a
//
LOCUS       DAZGTJ010000092        18224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000092 DAZGTJ010000000
VERSION     DAZGTJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.092"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(20..778)
                     /gene="aroD"
                     /locus_tag="ACHK8E_003992"
     CDS             complement(20..778)
                     /gene="aroD"
                     /locus_tag="ACHK8E_003992"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP0346196.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(809..1675)
                     /gene="ydiB"
                     /locus_tag="ACHK8E_003993"
     CDS             complement(809..1675)
                     /gene="ydiB"
                     /locus_tag="ACHK8E_003993"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP0346197.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1687..2952)
                     /locus_tag="ACHK8E_003994"
     CDS             complement(1687..2952)
                     /locus_tag="ACHK8E_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346198.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(3179..4393)
                     /locus_tag="ACHK8E_003995"
     CDS             complement(3179..4393)
                     /locus_tag="ACHK8E_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346199.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(4493..4849)
                     /locus_tag="ACHK8E_003996"
     CDS             complement(4493..4849)
                     /locus_tag="ACHK8E_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP0346200.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4985..5092)
                     /gene="rprA"
                     /locus_tag="ACHK8E_003997"
     ncRNA           complement(4985..5092)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK8E_003997"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5278..6390)
                     /gene="ydiK"
                     /locus_tag="ACHK8E_003998"
     CDS             complement(5278..6390)
                     /gene="ydiK"
                     /locus_tag="ACHK8E_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP0346201.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            6777..9833
                     /locus_tag="ACHK8E_003999"
     CDS             6777..9833
                     /locus_tag="ACHK8E_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP0346202.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9830..10240
                     /gene="menI"
                     /locus_tag="ACHK8E_004000"
     CDS             9830..10240
                     /gene="menI"
                     /locus_tag="ACHK8E_004000"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP0346203.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10340..10528
                     /locus_tag="ACHK8E_004001"
     CDS             10340..10528
                     /locus_tag="ACHK8E_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP0346204.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11077..11445
                     /gene="sufA"
                     /locus_tag="ACHK8E_004002"
     CDS             11077..11445
                     /gene="sufA"
                     /locus_tag="ACHK8E_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP0346205.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11454..12941
                     /gene="sufB"
                     /locus_tag="ACHK8E_004003"
     CDS             11454..12941
                     /gene="sufB"
                     /locus_tag="ACHK8E_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP0346206.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12951..13697
                     /gene="sufC"
                     /locus_tag="ACHK8E_004004"
     CDS             12951..13697
                     /gene="sufC"
                     /locus_tag="ACHK8E_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP0346207.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLL
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13672..14943
                     /gene="sufD"
                     /locus_tag="ACHK8E_004005"
     CDS             13672..14943
                     /gene="sufD"
                     /locus_tag="ACHK8E_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP0346208.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14940..16160
                     /gene="sufS"
                     /locus_tag="ACHK8E_004006"
     CDS             14940..16160
                     /gene="sufS"
                     /locus_tag="ACHK8E_004006"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP0346209.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16173..16589
                     /gene="sufE"
                     /locus_tag="ACHK8E_004007"
     CDS             16173..16589
                     /gene="sufE"
                     /locus_tag="ACHK8E_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP0346210.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16738..17742
                     /gene="ldtE"
                     /locus_tag="ACHK8E_004008"
     CDS             16738..17742
                     /gene="ldtE"
                     /locus_tag="ACHK8E_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP0346211.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17806..18042)
                     /gene="lpp"
                     /locus_tag="ACHK8E_004009"
     CDS             complement(17806..18042)
                     /gene="lpp"
                     /locus_tag="ACHK8E_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP0346212.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4785 a         4386 c         4677 g         4376 t
ORIGIN      
        1 cccgccgaaa tattattgct tatgcctgat gtaaaatagt taataccgta cgcaaatcat
       61 ttaccgagat ttgccctggc gcagaggctt tttttaccgc accaaaagtt gccgccgagc
      121 caaatacttc accagccaga cgagaaatta cgccagtttt tgccatcgac atcgtgatga
      181 ttggacgatc ggcatactgc tcctgcatct ccagggtcgc agcaagcaac gtcagcacat
      241 cgctggtact ttgcggcatc agcgcaatct taggaatatc ggcatcgaag gactgcattt
      301 tgcgcagacg ggcaatgatt tcttcggctt ccggcgtttt atggaagtca tggttggaca
      361 tgacaacttt cacatcatgc gcgtgggcgt aggcgacggt ttctttaacc tgatcatcgc
      421 cggtaaataa ctccagatcg attatatcaa ccaggccgct gtcgatggct gcacgattga
      481 gtgcaatata agcctcggtg gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg
      541 taaacagcag cggtttttct ggcatggttt cacggagaat ttttgctgcc gctatgacag
      601 actccacatt ggagagatcg gcatagtggt ccacacgcca ttccagaata tcaaagtccg
      661 cttcacgata ggcgagagct tcggatttca cgctggcgat atctttcgcc atcagcgaga
      721 cgatgatttt aggtgcgccc gtaccaatga cgagatcttt tacagttacg gttttcattt
      781 tttacccttt ctgcacgcgg tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat
      841 attccagagg gaaatctttg ccagtccata acgtgaactg ttcagcccct tgccacaaca
      901 acatgccgta tccatcaatc gttttgcaac ccgcttgttg cgcctgctgc aataacttcg
      961 tcatatgagg gttatacacg cattcagtga ccagaagtcc cggatgtaac agactgatat
     1021 cattaaccaa tgattcattc tcaaggggtt tcatacccac tttggtgcca ttggttaaaa
     1081 tgtcggcgga agccagggct tctgcaaagg cttgctgatc ggcgagatcg gtgactgtca
     1141 cgacgcaatc ggtgttttcg ttaacccgct gcgcgaaggc gagagcttta tcgaagaact
     1201 catcccgacg gttaaagagt ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa
     1261 ttgccgttga ggcaccaccg gcccctaata gcaccatcgt tttgcctttg atatcaaaac
     1321 cgctctcttt aatggcgcga atatgacccg tgccgtcggt gttatagcca cgcagatagc
     1381 catcatcatt aacgatggtg ttgatggctc ccaccagttt ggccgctggc gttaattcat
     1441 caacatattc acacgccagt tgtttgttcg gcattgatac accagttccg cgcattttga
     1501 gggcttttaa tccttcaatt gctccgggaa aactatcgtt atccacttca aaggccatat
     1561 aggtaaatgg caatcccgct ttttctaagg ctttattctg catttcgggc gataaactgt
     1621 ggcggatggg ataggccatc aacccaatca attcgtattt tgcggtaaca tccataatta
     1681 actcctttaa cctctatgct taatcgtgtt taaccgggtg ctaaatttat gctcgccaaa
     1741 ccgcacatca ttttcaggaa taatgaaaac gcggtaatag cggataaaaa caataattgc
     1801 ggtaatcagg gccagcagcg cgaaagtaaa atcgagaaca atgatatatt gcaggccgat
     1861 gttcgacaga taaccggtaa tcagcggaat aacaaagtta gccagtccac ccatcatcat
     1921 atatatactg gtgactttgg ctttgctttt tgggaaaaac tctgacatga ccgaaacgcc
     1981 gagctgtaaa atgccgccag ctgctgagaa accgataaca aaggctccgg cattacacac
     2041 caatggagaa gggtacaggt agataatggc agctgttatt gttgccagtg cagagttaaa
     2101 tacattagcc cagatgggcc ggaccatttt tttcagcagg gcggcaaaaa taaagacaca
     2161 gaccagcgaa cccatactgt aataagagat ggtttttaat gcctcagctt ctgacatacc
     2221 agcaaaagcc atcgcatatt tgggcatcca caccacaatc acataaaagg tcgcgaatgc
     2281 ggctacaccg aacagtactg atgaaacacc ttccagccag actaacggtt tgctgttcat
     2341 ttgcggtaat tcattagcta cgctggcgtc caccaactgg ctggggaatt tgcttttcaa
     2401 cagcatcagc gtgatcagta caaacagaat acccggaata atcagcccat agccgtacca
     2461 gatgttattg agcaacatat agctcaccag cattgggtag aacatttgcc cgaatgacac
     2521 catcgcttta accagtatga ccgccgaacc agaggctttc ggaaagcatt ccataagcgc
     2581 ggggtagcca cccgtatcca gcgctgagtt agcgatacct acgcacactg ccagacagta
     2641 ggcgagagtt aaattcgggc aagcaggaat accaaagaag aatagcagat acattattac
     2701 tgccattaat atcaccgccc gacgaccaaa cttatcggag atcacaccga agaataaaat
     2761 actgatcaat cgccccaatc cgataccgga aattaaatag gcaatgcccg cgttgtcagt
     2821 ggaaaacttt tctgccaaag atgtcatgtt ttgggcaagc gtaataacac taatgccgtg
     2881 caggaaatag ctgaagtaaa tacaaagaac agccaggata aatggcgtgc tgaaagcctt
     2941 attttgagac attttcacag ctccttgctg aaagataatt tcgttaccgt acgtattgtt
     3001 ctccgacggt ttacataatt actatactga agataatttt ccctgggtac atggcataaa
     3061 gtttatgttt ccaggaatat tgcatgtcat agtttgtttt tgatcgcgat tttccttagt
     3121 tagcgcagaa agagttattt atccgtaaga tcttgcatga cctgaaaaga atattgcatt
     3181 acccacccgg agcgacattt tcctttagcg agtgatgccg cgtttctttg cgactgcgta
     3241 agccaataaa cagtgccagt ataaagccga tggcagcgat ggcggtatcg aaccacataa
     3301 tatcggcaat acttctttgc gaaagatgag ccgtaatcaa cggaatagta aaggttgcaa
     3361 tgctgcccgc actgtaatag atccccgttg cttttccttt agcgtaggga aaacgttccg
     3421 ccattaacgt caggccaatt tgtacaacac cgcctgcgga ggtaaaaccg atgacgaaag
     3481 caaatattat caccacataa aatgtgggat gcaggcagac ggtgagcagg gcgataaatg
     3541 agataaaggt gtacagcatc agtaatgttg ttgggcgaac ggtattacga atgagtggag
     3601 cggtaataaa tacacaaagc agcgaaccca cggtgtagat actgagtaat ttgatcgaca
     3661 tggtgtatga catgccggcg acaaattgtc cgtattgtgc cagccactgg ctaaccagat
     3721 aaaacgttgc cattgagata tagccatata gggaataact ggctaaatcg ataattgaac
     3781 agcgatgttc cgtagagctg gtggttttct ttatgacagg taagtgacga cccggatggg
     3841 gtgggaacgt acaacggtat aaaagcagag cgttaataaa cataatgcct gcggcaatca
     3901 taaaggacca accgaaccac agttcagccc acactaacag gctgataatt agcggtaata
     3961 aaaattgtcc gctggaaaca aatgctttaa ttaaaatatt ggctgtaccc ggtgagcgtg
     4021 gaaaagcttc catcaaactg ggataggtgc ctgcatcgag aaaactgttt gccattcccg
     4081 ccagaaagcc aaaaacataa gcgataatga tgttattggt gtgcaggatg ccaaaaaaga
     4141 aggccatata gcagcacatc ccgagcatga taaaagggcg gcgaccaaag cgatcggata
     4201 ataatcctgc aaaaagcaag acacttaatc gaccaatgcc cagtgatgag ataactatcg
     4261 agacgcccgc ggcattagtc tgccaaagcg tctccagcga ggccatattc aggctcatca
     4321 aaatgacgcc cataccatgc accaggtaat taaaatacaa cccaagtgcg gtagggtaat
     4381 agggattttt cataggttct acttagcaaa ggtgctttaa aaagtgtaga gatgttgtgt
     4441 cagtatccgt tcattattgt tgtaacaaag acaattcctt tgtgtggaga aattaacaaa
     4501 gccgctccag atcgtgtgca tataattcag cggctaccga ttgatagatc ttccagtcaa
     4561 taaaattgcc atcagggtag acttgttgaa acacggttaa gtcggggaaa aaacgcatgg
     4621 tgttgttgcc gatgcggtta tttattttct ctatgatggc atgaagatgt ttttttcgtt
     4681 gctgacgcat agccaacagt tgctcgacta cacgatcagg aatggcgcat ttgccatttt
     4741 cccactgacg ccagctttca ctgtcatcgg tctgggcaat ccaggtcgcg cattcatcaa
     4801 tagtcatggc aaaaatatgg cgaagtgcct ggagttcata agcgttcatc gtgtaatggc
     4861 tgcatgaaat aaacaataaa atataaataa cattatcagc ctgctgacgg cttgaagaga
     4921 gtcacagtat cttgtgcaac attattggaa agcgtgaggg gcgagatagc aaagcggaaa
     4981 aatgttaaaa aaaagcccat cgtgggagat gggcaaagac tacacacagc aattcgttgt
     5041 ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc gtgagctaat
     5101 agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttaa aaactgtaaa
     5161 gaatttgcga caggtactac cagctgaatg gcggtaagca ggaaaatatg gggaagagca
     5221 agcaaatatc aattagcagg ggttaacaat caggcggcag agccgcctga tgagaaatta
     5281 cttattcgat ttctcgattt cgccaagttc ttcgagaatt tcttccggtt ggtccgtcgg
     5341 cggtgggact tcttccaccc acgcggcaaa aagacgccag gaaacggcta acagaaccgg
     5401 accaataaac aggccgatca tcccgaaagc aatcaaacca ccaataacac cagagaggat
     5461 caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga cgttatccag
     5521 cgtgccaacc acaccgctcc acactaacaa taccgttccc caggtggtat caccggtcca
     5581 gtagagccag ataatcgccg gaatcagtac cggcaacggt ccaagctgga caaggcagga
     5641 gaggatcatt aacaccgtga gcaaagttgc ataaggtacg ccggatacgg cgaggccgat
     5701 accgccaagc actgcctgta ctaacgccgt caccaccaca cccagcgcca ccgcgcggat
     5761 agcctgtgcc gccagtagca cagcagcatc accgcgaacg cctgccagac gggttgcaaa
     5821 atggcgaatg ccttgtgcca cctgttcacc gcgccagtac agcagggcac tgaagagaag
     5881 catcagcgca caatgcacca taaagcgccc gatatgcgcc gcctgcccaa cgaaccaggt
     5941 ggtggtggtg ccaatataag gacggacttt cgccatgatc gccgtacccc ccatatccag
     6001 caagttgtgc cagcctgcat atagcttcgc gccaatcacc ggaatggtat taagccacgc
     6061 cagatcgggt aacgtcatgt caccgctgga aatggcttta attagcggac cgctgccatc
     6121 gacaatactg ttaaccaaca aggcaatagg gatgataaac accatcacta ataacagcgt
     6181 catcaccaga acggcgagag agcggcggcc aaacatgatc ttttgcaaac gtaacaatac
     6241 cggccaggtg gcgataacca ctgtaccggc ccatgcaaag ccgagaataa agggttgaac
     6301 aatccacaga catgccacaa tcatgatggc taaaaacagc accgaaagca gaatttgtgc
     6361 gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt gtgcgtcaaa
     6421 tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa tttcagcggt
     6481 ttttaacagg tcgattctgc ccttaattct gatgggcgtg taagaaaaaa atgtggtaca
     6541 acaactcagg caacacgcaa acgatttact cgtcgtattt caaattgcat gtgctgcggc
     6601 tacgttcgtt caccccagtc acttactttt gtaagctcct ggagttcact cgcttgccgc
     6661 cttcctgcaa ctcgaaatcc atagagtaaa caaacatcac ctttcagtgc gctattagca
     6721 gcctgaaaga acagtgtatc gatctacatc acagaccgca ggaaagggtc aatataatga
     6781 ttccacagat ttcccaggca cccggcgtcg ttcaattggt gcttaatttt ttgcaagagc
     6841 tggagcaaca gggttttact ggcgatacgg cgacaagtta tgccgatcgt ctgacaatgt
     6901 cgaccgacaa cagtatttac caacttctcc ccgatgcggt ggtatttccg cgttcaaccg
     6961 cagatgtggc gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta
     7021 ccccccgcgg cggcggcacc ggcactaacg gtcaggcgct caaccagggg attattgttg
     7081 atatgtcccg ccatatgaac cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg
     7141 ttgaggccgg ggtgataaaa gatcaactca atcagtacct gaaaccgttc ggctactttt
     7201 ttgcgccgga actttcgacc agcaaccggg caacgctcgg tgggatgatc aataccgatg
     7261 catccggtca gggatcgctg gtctatggca aaacgtcaga tcacgtactt ggcgtacgcg
     7321 cggtgttgtt ggggggcgat attctcgata cgcaaccttt acccgtcgaa ctggcggaaa
     7381 cgctgggtaa atccaatacc acaatcgggc gaatttataa cacggtttat caacgttgcc
     7441 gtcagcaacg ccagttaatc atcgacaact tccccaagct taaccgcttt cttaccggtt
     7501 acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt cgacctgacg cgcattctga
     7561 cgggttcaga agggacgctg gcctttatta ccgaagcgcg actggatatt acgcgcttgc
     7621 ctaaagtgcg ccgtctggtg aacgtcaaat atgactcttt tgactccgca ctgcgtaacg
     7681 cgccgtttat ggttgaggcg cgggcgcttt cggtagagac ggtggactca aaagtgctga
     7741 atctggcgcg ggaagatatt gtctggcatt ccgtcagcga gttgattacc gatgtgcctg
     7801 acaaagaaat gctcgggctg aacattgtgg aatttgctgg tgatgatgag acgctgattg
     7861 atgagcgggt aaatgcactc tgtgcgcggt tggatgaact gattgccagc caacaggcag
     7921 gtgtgattgg ctggcaggtg tgccgcgagc tggcgggtgt tgaacgtatc tatgcgatgc
     7981 gcaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca attccgtttg
     8041 ctgaggatac ctgcgtaccg ccggaacatc tggccgatta tattgctgaa tttcgcgcgc
     8101 tgctcgacag ccacggctta agctacggta tgttcggtca cgtcgacgca ggtgtcttgc
     8161 acgtccgtcc tgcgctggat atgtgcgatc cgcaacaaga gattttgatg aagcaaatct
     8221 ctgatgacgt agtggcgctt accgcgaaat acggtggttt gttgtggggc gaacacggca
     8281 aaggttttcg tgccgaatac agccctgcgt ttttcggtga ggaacttttt gcagaactgc
     8341 gcaaagtgaa agcggcattt gacccgcata accgactcaa cccagggaag atttgcccgc
     8401 cagaaggtct cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt acattcgatc
     8461 ggcagatccc cattgcggta cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg
     8521 gtttatgctt caactttgat gcccgtagtt cgatgtgtcc gtcgatgaag atcacccaga
     8581 accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg cgcttgttgg
     8641 cggatcgcgg cgttgaccca ctcaaactgg aacaagaact gcctgaatcc ggcgtcagtt
     8701 tgcggacgtt aattgcccgc acgcgcaata gctggcatgc gaataaaggc gaatacgact
     8761 tctcacacga agtcaaagag gcgatgtcgg gctgtcttgc ctgtaaagcg tgttcgaccc
     8821 agtgccccat caaaattgat gtgccggagt ttcgctctcg ttttctgcag ctctatcaca
     8881 cccgttattt acgcccgctt cgtgaccacc tcgttgctac ggtcgagagc tacgcaccgc
     8941 tgatggcacg cgcgccgaaa acctttaact tcttcattaa ccagccgctg gtgcgcaagc
     9001 tctcggaaaa acatatcggc atggttgatt tgccactgct gtcagtcccc tcgctacaac
     9061 aacaaatggt ggggcatcgc tcggcgaata tgacgctgga acagcttgaa gctctcaatg
     9121 cagagcagaa agcgcgcacg gtgttggtgg tgcaggaccc ctttaccagc tattacgatg
     9181 cgcaagtggt ggcggatttt gtccgtctgg ttgaaaaatt aggtttccag cctgtgttac
     9241 tgccattttc gccaaatggc aaagcccagc atatcaaagg ctttcttaat cgttttgcga
     9301 agacggcgaa aaagacggcg gatttcctca accgtatggc gaagctgggg atgccaatgg
     9361 tgggcgtcga tccggcgctg gtactttgtt atcgcgatga atataaactg gcgctgggcg
     9421 aggaacgtgg cgagtttaac gtcttactgg cgaatgaatg gctggcaagc gcacttgact
     9481 cacagccagt ggctacagtc agcggtgaat catggtattt ctttggtcac tgtaccgaag
     9541 ttaccgcttt gccgggcgcg ccagcacaat gggttgcgat atttgcccgt tttggcgcga
     9601 aactggaaaa tgtcagcgtg gggtgctgcg gcatggcagg gacttacgga catgaagcga
     9661 aaaaccataa aaattcgctc gggatctatg agttatcctg gcatcaggcg atgcagcgac
     9721 tgccgcgtaa tcgctgtctg gcgaccggat attcctgccg tagccaggta aaacgggtcg
     9781 aaggcacggg ggtacgccat cctgtgcagg ctttactgga gattattaaa tgatatggaa
     9841 acgaaaaatc accctggaag cactgaatgc catgggtgaa ggaaacatgg tggggttgct
     9901 ggatattcgc tttgaacata ttggtgatga cacccttgaa gcgacaatgc cagtagactc
     9961 gcggacaaag cagcctttcg ggttgctgca tggaggggca tccgtggtac tggccgaaag
    10021 tatcggttcc gttgccggtt atttatgtac cgaaggtgag caaaaggtgg ttggtctgga
    10081 aatcaatgct aaccacgtcc gctcggcacg agaagggcgg gtgcgcggcg tatgcaaacc
    10141 gttgcatctc ggttcgcgtc accaggtctg gcagattgaa atcttcgatg agaaagggcg
    10201 tttgtgctgt tcgtcacgat taacgaccgc cattttgtga cgttgtcacc agaaaagtgt
    10261 gacgcatatt gcttttggtc attatctgca cagtaaacgg tggtatactg tgcaggtctt
    10321 gtctaaaagc gaggatgcta tgtctactca acttgatccc acccagctgg ccattgaatt
    10381 tttacgccgt gaccagagca atctttcacc tgcccaatat ctcaaacgtc tgaaacaact
    10441 agaacttgaa tttgctgacc tgcttactct ctcttctgct gaattaaaag aagaaatcta
    10501 ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat gccgcaattt tgccaaatcg
    10561 acctggatta acctccgtcc aggttcgatg accttcttaa tactgcttat ctatccgcct
    10621 gatattgttc aaaaataata ctaatcgcag tttgtgttaa aacggcgggt tagctttatg
    10681 agattattct tatcgcccct tcaagagcta agccactgtg agtgccggag ataagcgccg
    10741 gatggggtag aaacccttaa gcctgtgtcg cacagactta agggttttct tatttctgaa
    10801 cgctgcactt ttttacctat cccttaaata gtcattttca atacaacttt ttctttttca
    10861 tcacagcctt aatgctcctt agataataat cattatctaa caatgagata cctaattctt
    10921 agcgtgcctg ttaacccaca catcagggtc tatgcttatt aaaagcaggt aaaaagcatg
    10981 ttttacgcta aagtccctgc gtttgctggg ttgaactgat aatcattatc actaacatgc
    11041 tgttatacgc tgaaagcgat gaagtgaggt aaatcgatgg acatgcattc aggaaccttt
    11101 aatccacagg atttcgcctg gcagggctta acgctgacac ccgcagccgc tgtccacatc
    11161 cgcgagctgg tggcaaagca gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg
    11221 ggctgcgcgg gctttggcta tgtgctcgac agtgttagcg agccggacaa agacgatctg
    11281 ctgtttgaac acgacggcgc gaagctgttt gtcccgctgc aagcgatgcc gtttattgat
    11341 ggcacggaag tcgatttcgt tcgtgaagga cttaatcaga tattcaaatt tcacaaccct
    11401 aaagcccaga atgaatgtgg ctgtggcgaa agctttgggg tataggcggt actatgtctc
    11461 gtaatactga agcaactgac gatgtcaaaa cctggaccgg cggcccgctg aattataaag
    11521 aaggattctt cacccagtta gccaccgatg agctggcaaa ggggataaac gaagaggtgg
    11581 tgcgcgcaat ttcggcgaag cgtaatgagc cggagtggat gctggagttt cgtctaaatg
    11641 cctatcgcgc atggctggag atggaagaac cgcactggtt gaaagcgcac tacgacaagc
    11701 tgaattatca ggattacagc tactattcgg caccatcgtg cggtaattgt gacgacactt
    11761 gtgcgtctga acccggcgcg gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg
    11821 tggaggcggc gtttgagcag ttgggcgttc ccgtgcggga aggcaaagag gtggcagtgg
    11881 atgccatttt cgactctgtt tcggttgcca ctacttatcg cgaaaaactg gcggagcagg
    11941 gaattatttt ttgttccttt ggcgaggcga tccacgatca cccggaactg gtgcgtaaat
    12001 atctcggcac cgtggtgccg gggaatgaca acttctttgc cgcacttaat gcggcggtag
    12061 cctctgacgg tacgtttatt tatgtgccta aaggcgtgcg ttgcccgatg gaactttcca
    12121 cctattttcg cattaacgcg gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg
    12181 acgaagacag ctacgttagc tacattgaag gctgttccgc tccggtgcgt gacagctatc
    12241 agttacacgc ggcggtggtg gaagtcatca tccataaaaa cgccgaggtg aaatattcca
    12301 cggtacaaaa ctggttccct ggcgataaca acaccggcgg tattctcaac ttcgtcacca
    12361 agcgtgcgtt gtgcgaaggc gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt
    12421 cagcgattac gtggaaatat cccagctgca ttttgcgcgg cgataactcc attggtgagt
    12481 tttactcagt ggcgctgacc agcggtcatc agcaagcgga taccggcacc aagatgatcc
    12541 acatcggtaa aaacaccaaa tcgaccatta tctcgaaagg gatctctgcc ggacatagtc
    12601 agaacagtta tcgcggctta gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca
    12661 ctcagtgcga ttcaatgctg attggcgcta attgtggggc gcataccttc ccgtatgtcg
    12721 aatgtcgcaa taacagcgct cagctggagc acgaggcaac gacatcacgt attggtgaag
    12781 atcaactgtt ttactgcctg caacgcggga tcagcgaaga agatgccatc tcgatgattg
    12841 ttaacggttt ttgcaaagac gtgttctcgg aactgccgct ggaatttgcc gttgaagcac
    12901 aaaaactcct cgccatcagt cttgaacaca gcgtcggata aggaataaac atgttaagta
    12961 ttaaagattt acacgtcagc gtggaagata aagctatcct gcgtggatta agcctcgacg
    13021 ttcgtcccgg cgaagttcac gccattatgg ggccaaacgg ttctggcaaa agtaccttat
    13081 tggcaacgct tgccgggcga gaagattatg aggtgacggg cggcacggtt gagttcaaag
    13141 gcaaagattt gcttgcgctg tcaccggaag atcgtgcagg tgaaggcatc tttatggcct
    13201 tccagtatcc ggtggaaatt cccggcgtca gtaaccagtt tttcctgcaa acggcactta
    13261 atgcggtgcg cagctatcgc ggccaggaaa cgctcgaccg ctttgatttt caggatttga
    13321 tggaagagaa aatcgctctc ctgaagatgc cggaagattt attaacccgt tcggtaaacg
    13381 ttggtttttc cggcggtgag aaaaagcgca acgatatttt gcaaatggcg gtgctggaac
    13441 cggagttatg cattcttgat gagtcagact ccgggctgga tattgacgca ttaaaagtgg
    13501 ttgccgatgg cgtgaactcg ctgcgtgacg gcaagcgttc attcatcatt gttacgcact
    13561 accaacgcat tctcgactac atcaagcctg attacgtcca tgtgctgtat cagggacgaa
    13621 ttgtgaaatc cggcgatttc acgttggtca aacaactgga ggagcagggt tatggctggc
    13681 ttaccgaaca gcagtaacgc actgcaacag tggcatcatt tgtttgaagc tgaaggggcg
    13741 aaacgctccc cgcaagcaca gcagcattta caacaattgc tgcgtaccgg actgccgaca
    13801 cgtaaacatg aaaactggaa atatacgccg ctggacgggc tgaccaatag ccagtttgtc
    13861 agcattgcgg gggagatatc cccacagcag cgtgatgcct tagcgttaac attagacgcc
    13921 gtgcggctgg tgtttgtcga tggacgttac gtgcccgcac tgagcgatgc aactgaaggc
    13981 agcggatatg aagtgagcat taacgacgac cgtcaggggt tacccgatgc tattcaggcg
    14041 gaagtgtttc tgcatttgac ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag
    14101 cgcggtcaac ggccggcaaa gccattgctg ttaatgcata tcacccaggg cgtggcaggt
    14161 gaagaggtga atactgccca ttaccggcat catctggatc tggcggaagg tgccgaagca
    14221 acggtgatcg aacattttgt cagccttaat gatgctcgtc acttcaccgg cgcacggttc
    14281 actatcaacg tcgcagcgaa cgcccatttg cagcatatca agctggcgtt tgaaaacccg
    14341 ctcagtcacc actttgctca taacgatttg ttgctggctg acgatgccac cgcatttagc
    14401 cacagtttcc tgctgggtgg cgcagtatta cgacacaaca ccagtacgca actcaatggc
    14461 gaaaacagca cgctgcggat caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat
    14521 actcgtacct ggttggaaca caataaaggt ttttgtaaca gccgacagtt gcacaaaact
    14581 atcgtcagcg acaaaggccg cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc
    14641 atcaaaacgg atggtcagat gaccaacaat aatctgctga tgggcaaact ggcggaagtg
    14701 gatacgaaac cgcagctgga aatctatgca gatgatgtga aatgcagcca cggcgcgacg
    14761 gtggggcgta ttgatgacga acagatgttt tatctgcgct cgcgcgggat caatcagcaa
    14821 gatgcccagc agatgatcat ttacgccttt gctgccgaac tgacggaagc actgcgtgat
    14881 gaggggctta aacagcaggt gctggcccga atcggtcaac gactgccagg aggtgcaaga
    14941 tgactttttc cgtcgacaaa gtgcgggccg actttccggt gctttctcgt gaggtaaacg
    15001 gtttgccgct ggcttatctc gacagcgccg ccagtgcgca gaaaccgagc caggtgattg
    15061 atgccgaggc tgagttttat cgtcatggct acgcggcggt gcatcgcggt attcatacct
    15121 taagcgccca ggcgaccgag aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta
    15181 atgcccgttc ggcggaagag ctggtgttcg tccgcggcac gacggaaggg atcaatctgg
    15241 tcgccaatag ctggggcaac agcaatgtgc gagcgggcga taacatcatc atcagtcaga
    15301 tggagcacca cgctaacatt gtcccctggc agatgctttg tgcacgcgtt ggcgcagagc
    15361 tgcgtgtgat cccgctcaac cccgacggta ctctgcaact ggagacgctg cctacgctgt
    15421 ttgatgagaa aactcgtctg ctggcaatta ctcatgtctc caacgtgctt gggacagaaa
    15481 atccgctggc ggagatgatc acacttgcgc atcaacatgg cgcaaaagtg ctggtggatg
    15541 gcgctcaggc ggtgatgcat catccggtgg atgttcaggc gctggattgc gatttttacg
    15601 tgttctccgg gcataaactg tatggcccta ccggaattgg cattctttat gtcaaagaag
    15661 ccttgttgca ggagatgccg ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc
    15721 tgagtgaagg cactacctgg accaaagcac catggcggtt tgaagccggt acacccaata
    15781 ccggaggcat cattggtctt ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata
    15841 acatagccga gtatgaacag aatctgatgc attacgcgtt atcacagttg gaatctgtac
    15901 cggatctcac tctctatggc ccacaaaaca ggcttggcgt tattgctttt aatctcggta
    15961 aacaccacgc ctatgatgtt ggcagttttc tcgataatta cggcattgct gtgcgtaccg
    16021 gacatcactg cgcaatgcca ttaatggcct attacaacgt ccctgcgatg tgtcgggcat
    16081 cgctggccat gtataacacc catgaagaag tggatcgtct ggtgaccggc ctgcaacgta
    16141 ttcaccgtct gctgggataa cagggaggca ctatggcttt attgccggat aaagaaaaat
    16201 tgctgcgtaa ttttttacgc tgcgccaact gggaagagaa atatctctac attattgagt
    16261 tgggtcagcg tctgccagaa ttacgtgacg aagacaaaag cccgcaaaat agcatccagg
    16321 gctgccagag tcaggtgtgg attgtgatgc gtcaaaatgc gcagggaatt attgaattac
    16381 agggcgacag cgatgcggcc attgtaaaag ggcttattgc ggtcgtcttt attctttacg
    16441 atcaaatgac gccgcaggat attgtcaatt tcgatgtgcg cccgtggttt gaaaaaatgg
    16501 cgctcaccca acatctcacc ccatcacgct cacaaggtct ggaagcgatg attcgcgcaa
    16561 ttcgcgccaa agccgctgca cttagctaaa ctaaagggac agctttcatc cggttggctc
    16621 acattgccgg gtggtcaatg tttacactgg cctgcacagg agtgttttgg tttcaggtga
    16681 acataagcgc catccgacgt ttcagcgtga gtctccggca aatacaggag gtttacaatg
    16741 aaatgcgcgt ctttgcttac actcacgctt atcggcgctt ttagcgccat ccaggctgcc
    16801 tgggcggttg attatccgct accaccaacc ggcagtcgac tggttgggca aaatcaaacg
    16861 tatacggtgc aagaagggga taaaaacctt caggccatcg cccgacgttt tgatactgcg
    16921 gcaatgttga tccttgaagc caataacact atcgccccgg tgccaaaacc cggtacgacg
    16981 ataactattc cttcacaact attattacct gatgcgccgc gtcaggggat tattgtaaac
    17041 cttgcagagc tgcgccttta taattatccg ccgggagaaa atatcgtgca ggtttatccg
    17101 ataggtattg gtttgcaggg gctggaaacg ccggtgatgg aaacgcgtgt ggggcagaaa
    17161 atccctaacc caacctggac gcctacggcg ggcattcgtc aacgttcgct ggaacgtggc
    17221 attaaattac caccagtcgt tcctgccggg cccaataacc cactaggacg ttacgcactg
    17281 cgcctcgcgc atggtaatgg cgaatacctc attcacggca ccagtgcgcc ggacagcgtc
    17341 ggtttgcgcg tcagttcagg gtgtattcgc atgaatgcac cggatattaa agccttattc
    17401 tccagcgtgc ggacgggaac gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg
    17461 gagcctaacg ggatgcgtta tgttgaagta catcgaccac tatcggcaga agaacagcag
    17521 aacgttcaga caatgccata cacactgcca gcaggcttta cgcaatttaa agacaataag
    17581 ggtgtagatc agaaattagt cgataaagcg ttgtatcgtc gggcagggta tccggttgcg
    17641 gtgagcagtg gagcaactcc cgcagccagc aatgcgcctt cagtagagtc agcgcagaat
    17701 ggtgaaccag agcaagggaa tatgttacgc gcgacgcagt agcggtaaat ggcaggcaaa
    17761 aaaaatggcg cacaatgtgc gccatttttc acttcacagg tactattact tgcggtattt
    17821 agtagccatg ttgtccagac gctggttagc acgagctgcg tcatctttag cagcctgaac
    17881 gtcggaacgc attgcgttca cgtcgttgct cagctggtca actttagcgt tcagagtctg
    17941 aacgtcagaa gacagctgat cgattttagc gttgctggag caacctgcca gcagagtaga
    18001 acccaggatt accgcgccca gtaccagttt agtagctttc attattaata ccctctagat
    18061 tgagttaatc tccatgtagc gttacaagta ttacacaaag ttttttatgt tgagaatatt
    18121 tttttgatgg gaaggcactt atttttgatc gttcgctcaa agaagcatcg aacgctgggt
    18181 tttagataaa aaaattgaga gaaaacagga tgcttccatc ggat
//
LOCUS       DAZGTJ010000093        18174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000093 DAZGTJ010000000
VERSION     DAZGTJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.093"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(142..1170)
                     /gene="epmB"
                     /locus_tag="ACHK8E_004010"
     CDS             complement(142..1170)
                     /gene="epmB"
                     /locus_tag="ACHK8E_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0346213.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1212..1778
                     /gene="efp"
                     /locus_tag="ACHK8E_004011"
     CDS             1212..1778
                     /gene="efp"
                     /locus_tag="ACHK8E_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0346214.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1830..1955
                     /gene="ecnA"
                     /locus_tag="ACHK8E_004012"
     CDS             1830..1955
                     /gene="ecnA"
                     /locus_tag="ACHK8E_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0346215.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2066..2212
                     /gene="ecnB"
                     /locus_tag="ACHK8E_004013"
     CDS             2066..2212
                     /gene="ecnB"
                     /locus_tag="ACHK8E_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0346216.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2387..2704
                     /gene="sugE"
                     /locus_tag="ACHK8E_004014"
     CDS             2387..2704
                     /gene="sugE"
                     /locus_tag="ACHK8E_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0346217.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2705..3238)
                     /gene="blc"
                     /locus_tag="ACHK8E_004015"
     CDS             complement(2705..3238)
                     /gene="blc"
                     /locus_tag="ACHK8E_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0346218.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3327..4460)
                     /locus_tag="ACHK8E_004016"
     CDS             complement(3327..4460)
                     /locus_tag="ACHK8E_004016"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0346219.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(4523..4882)
                     /gene="frdD"
                     /locus_tag="ACHK8E_004017"
     CDS             complement(4523..4882)
                     /gene="frdD"
                     /locus_tag="ACHK8E_004017"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0346220.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4893..5288)
                     /gene="frdC"
                     /locus_tag="ACHK8E_004018"
     CDS             complement(4893..5288)
                     /gene="frdC"
                     /locus_tag="ACHK8E_004018"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0346221.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5299..6033)
                     /gene="frdB"
                     /locus_tag="ACHK8E_004019"
     CDS             complement(5299..6033)
                     /gene="frdB"
                     /locus_tag="ACHK8E_004019"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0346222.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6026..7834)
                     /gene="frdA"
                     /locus_tag="ACHK8E_004020"
     CDS             complement(6026..7834)
                     /gene="frdA"
                     /locus_tag="ACHK8E_004020"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0346223.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8159..9136
                     /gene="epmA"
                     /locus_tag="ACHK8E_004021"
     CDS             8159..9136
                     /gene="epmA"
                     /locus_tag="ACHK8E_004021"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0346224.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9355..10857
                     /gene="yjeM"
                     /locus_tag="ACHK8E_004022"
     CDS             9355..10857
                     /gene="yjeM"
                     /locus_tag="ACHK8E_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0346225.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            10920..11234
                     /gene="yjeN"
                     /locus_tag="ACHK8E_004023"
     CDS             10920..11234
                     /gene="yjeN"
                     /locus_tag="ACHK8E_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP0346226.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFHGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            11231..11545
                     /locus_tag="ACHK8E_004024"
     CDS             11231..11545
                     /locus_tag="ACHK8E_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP0346227.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11576..14899)
                     /gene="mscM"
                     /locus_tag="ACHK8E_004025"
     CDS             complement(11576..14899)
                     /gene="mscM"
                     /locus_tag="ACHK8E_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0346228.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14921..15889)
                     /gene="asd"
                     /locus_tag="ACHK8E_004026"
     CDS             complement(14921..15889)
                     /gene="asd"
                     /locus_tag="ACHK8E_004026"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0346229.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            complement(15986..17038)
                     /gene="rsgA"
                     /locus_tag="ACHK8E_004027"
     CDS             complement(15986..17038)
                     /gene="rsgA"
                     /locus_tag="ACHK8E_004027"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0346230.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17133..17678
                     /gene="orn"
                     /locus_tag="ACHK8E_004028"
     CDS             17133..17678
                     /gene="orn"
                     /locus_tag="ACHK8E_004028"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0346231.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17889..17964
                     /locus_tag="ACHK8E_004029"
     tRNA            17889..17964
                     /locus_tag="ACHK8E_004029"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17922..17924,aa:Gly,seq:gcc)
BASE COUNT         4207 a         4647 c         4789 g         4531 t
ORIGIN      
        1 taaatagccg tgctcactct atataaacag aaaagaaagg ttaattattg gtgttggtta
       61 tataaaaagt aataacagca atgtatatga ataattaaag gttatgcaac gtgcaaagat
      121 taaacttccc tgtttaatca gttactgctg gcgtagctgg agatccagcg gcgttttgct
      181 gggttcgccg ccaatttctc gcgccagttt aggcaccaga tatcccgaca ccagtgtcag
      241 caactcacgc ataatctgcc gtgcttcgtc atcactcacc ataaaatgcg ccgcgccctg
      301 tactttatcg agcacatgca ggtaatacgg catcacgccc gcatcgaaca acgcattact
      361 caggtttgcc agcgtttgcg cgttatcgtt cacaccacgt aacagaacgc tctggttgag
      421 caaagtaacg cctacccggc gcaacttagc catcgcctga cggaatgttt catctacctc
      481 attggcatgg ttaatgtgat tcaccagcaa gatttgcagc gtagaatgag aaaagcgttc
      541 aaccagcgca tctgtgatgc gtgccgggat cacaatcggc agacggctgt gaatccgcag
      601 acgtttgata tgcgggatgg cttccagttg tgtgagcaac cagtccagct cgtgatcttt
      661 cgccatcagc ggatcgccgc cggagaaaat catctcgtcc agttccggat gcgcagcaac
      721 atactcaagt gcagtctgcc agttacgctt gttgccctga ttttcggcat aggggaagtg
      781 gcgacggaag caatagcggc aatttaccgc gcagccgcct ttgaccagca aaagcgcccg
      841 gttgtggtat ttatgcaaca aacctggcac tacgctgtgc tgttcttcca gcgggtcggt
      901 ggagaatccg ggcgcgacaa caaactcatc ttgcgaggta agtacctgac gcaaaagcgg
      961 atcgttcgga ttgcctttct ccatgcgatc gataaatgag cggggcacac gcagggcaaa
     1021 cagctttttg gcgctgcgcc cggccaacag tttttcgtcc gcgtctatat tcaaaagacg
     1081 cagaagttca tcaggatcgg tcacaacatc ggcaagttgc gttaaccaat cttctctgga
     1141 tggggtattt agggttacaa tatgcgccat tttgtggctt agctaccaat taacaaattt
     1201 cagagggcct tatggcaacg tactatagca acgattttcg tgctggtctt aaaatcatgt
     1261 tagacggcga accttacgcg gttgaagcga gtgaattcgt aaaaccgggt aaaggccagg
     1321 catttgctcg cgttaaactg cgtcgtctgc tgaccggtac tcgcgtagaa aaaaccttca
     1381 aatctactga ttccgctgaa ggtgctgatg ttgtcgatat gaacctgact tacctgtaca
     1441 acgacggtga gttctggcac ttcatgaaca acgaaacttt cgagcagctg tctgctgatg
     1501 caaaagcaat tggcgacaac gccaaatggt tgctggatca ggcagagtgt atcgtaactc
     1561 tgtggaatgg tcagccgatc tccgttactc cgccgaactt cgttgagctg gaaatcgttg
     1621 ataccgatcc gggcctgaaa ggtgataccg caggtactgg cggcaaacca gctaccctgt
     1681 ctactggcgc tgtggttaaa gttccgctgt tcgtacagat cggcgaagtc atcaaagtgg
     1741 atacccgctc tggtgaatac gtctctcgcg tgaagtaatg cggttgtggt gcggcctgca
     1801 ggctgcacca tcacttattc aggtcagaga tgatgaaacg ccttatcgtt cttgttttgc
     1861 ttgccagtgc gctgctcacg ggctgtaaca ccgctcgcgg ttttggtgaa gacattaaac
     1921 atctcggcaa ctccatctcc cgcgctgcca gctaattttt cttctcctcc gaaaaatcat
     1981 cagatttcca tcatttttgg cgatgttgtc tattattaat ttgctatagg caaacataaa
     2041 taacattacc taaaaggaag acgttatggt gaagaagaca attgcagcga tcttttctgt
     2101 tctggtgctt tcaacagtat taactgcttg caacaccacg cgtggcgttg gtgaagacat
     2161 ttctgatggc ggtaacgcga tttctggcgc agcaacgaaa gcgcagcagt aagcaataac
     2221 ggtacgacag ctgtgtcgtg ccgtttgttt ttttgcgata gtcacaaagg taatagttga
     2281 aattcccctg ccacctggca aaatatccgt tcaaccatca gctttgcagg acgacctgca
     2341 aacgcctctt ttcaccgggg acggccccaa ttctccggag cctgatatgt cctggattat
     2401 cttagttatt gctggcctgc tggaagtggt atgggccgtt ggcctgaaat atacccacgg
     2461 ctttagtcgt ttgacgccaa gtgttattac tgtgacggcg atgattgtca gtatggcgct
     2521 acttgcctgg gcgatgaaat cgttaccagt agggacggct tatgccgtgt ggacgggtat
     2581 tggcgcagtc ggcgcggcca tcaccggcat tgtgctgctc ggcgagtccg ctaacccgat
     2641 gcgcctggcg agtctggcgt taatcgtatt ggggattatt ggtctgaaac tcagcactca
     2701 ctaactaact accgggctgc tgtacccaaa taaacttact gacatcaaac ccttcccggg
     2761 tcgcgactgc cagcatctcc tgtttcactt cgtcagaaat ggttggcgtg cgggagagta
     2821 tccacaggta gtcgcggtcc ggaccgcaaa ccagcgcatg gcggtattcc cgatcgagtg
     2881 caataacgtt ataaccgcca tagaaaggac caaagaatga cactttcagc gcagcgcgag
     2941 ttggtgcgcc ggtaaagtac gctttccctt cactctgctg ccacatttct ctgtcagggt
     3001 tatagccttt attaatgaca ttcaggccgc cgtcatcacg caggctgtat gttgcggtga
     3061 ctttttccag tccacgttca aaacggtgat caaaacgggc aatctcatac caggtaccga
     3121 gatagcgttt agcatcgaaa ttattcacta cggtcacgcc acggggcggc gtaggagaac
     3181 tgcaggcaac gaccagaaat gcagctgtcg ctgcggcaac gagagggagc aggcgcataa
     3241 atgtttcctt actggttttt ttctaagtgt agatgacagc aaggaaaagc ggagaaaagg
     3301 tccaaaaatt cggacccgat gaaattttac tgtagagcgt tgagaatctg acaggcggcg
     3361 gcgactctcg ctggattggg atagtttttg ttagccagca tcacgatacc cagctctttt
     3421 tctggaataa acgcgacata gctaccaaat ccgccggtcg cccccgtttt atgtacccat
     3481 gatgcgcgta ctgcaggagt tgggggcgta atcgctttta cggggcgtgc tgccagtgca
     3541 attttattgt cactgccgtt aatgatgatg tcaggattga ccggccagtc cagcatttcc
     3601 cagcccaggc cctgatacat atcgccggtt tgccagtagc gagattgtgc cagttgtatc
     3661 ccttgttgaa gcgttttctc agtgatatca aggggtttta aattgctttg cacccagcgg
     3721 gccatatctt caatggtcga cttcacacca taagcctcag catctaacgc ccctggcgaa
     3781 acatgcactg ccttaccttc gcgatatccc caggcgtaat tcttttcttc tgcggacggc
     3841 acattaatcc acgtatgagt gagtttgagt ggctggaaaa cacgagtttg catcgcctgt
     3901 tcaaagctca aaccggatgg tttcaccgcc agtgcaccga acaacccaat actggcgttg
     3961 gcatacagac gttgtgttcc tggagcccat gcaggttgcc agttttgata gaagcgcagc
     4021 aagtcgcttg aggatttaac gtcatccggc acctgcaatg gcaggccgcc agcggtgtag
     4081 gtcgcgagat gtaatagtgt gatcccattc cattgtttag cggtaagttc aggccagtat
     4141 tttgtcgcgg gatcgcttaa cttgatttcc cctcgagcaa tagcgtcgcc accaagcacg
     4201 cccgtaaatg ttttgctgac cgaacctaac tcaaacaacg tttgctgtgt gacgggctgc
     4261 tttttggcga tgtccgcata gccccaggta aagtaataag gtttaccctg ataaattacc
     4321 gccaccgcta taccggggat cttttgttgc tctataagcg gggtaattgt gcgatgcaca
     4381 atatcgttga tttgttgagg ggcagcaaat gtggagcaag aggcggtaat taataaggcg
     4441 cagagcgtca ttttgaacat agggtctggt ttccatacaa aacggcccgc cataggcggg
     4501 ccggatttac attggcgatg cgttagattg taacgacacc aatcagcgtg acaactgtca
     4561 ggatagcagc cagaccgtag aaaacccatt tgcccgcagg tacgtggatt ttcagatcgt
     4621 gcatcgcgtg gtgcatacgg tgtaaaccac accacagcgg cagaacgatc atcaggaaca
     4681 ggaatacgcg accaatgaag ctctgcgcga acgccagaac gcgctcgtag ctcagtgcgt
     4741 cacccggaaa cagccccagt ggcagcagaa taccaaccag caggatcatc accggcgcaa
     4801 taattgcgct ccacatacca ccggccccga agaggcccca gaataccggt tcgtcagaac
     4861 gctttggatt tggattaatc atctcaggct ccttaccagt acagggcaac aaacaggatt
     4921 acgatggtgg caaccacagt taccgcccag aggcttttga taattggctc tggtcccatt
     4981 ttttcgtctt ttacaatgat attggccgct tttggtgcca gctcaaacca ggttttggtg
     5041 tgcagcaggg ctgccgccag agtgatcagg ttaatgatca cgataaccgg gttttgtaaa
     5101 aagtcgacga atcccgccca ggcttccggg ccatttttca gggcaaacag cccgaaaatc
     5161 agttcaatgc tgaaccacac agccggaacc gccgtgcctt cgcgcagcat gtagaagcga
     5221 taaaacggca attttttcca ccaggtggac gtcattggcc gtacatacgg tttacgttta
     5281 gtcgtcatgt tgcactcctt agcgtggttt cagggtcgcg ataagaaagt ctttcgaact
     5341 ttctactttg ccctgctgaa tggccgcagc cggatcgacg tgtttcgggc agacttcgga
     5401 gcagtagccc acgaaagtac agctccatac gccgttctgg ctgttcaact gcgccatacg
     5461 ctccttctta ccgtggtcgc ggctatcttc gttataacga tgcgccagcg taatggcagc
     5521 cggaccgatg aactctgggt tcaggccaaa ctgcggacac gcggcgtagc acagaccaca
     5581 gttgatgcaa ccggagaact ggtgatactt ggccatctgc gccggggtct ggatgttagt
     5641 accctgatcc gcggtgcggg agttgccgat gatgtacggt ttgatcgctt ccagactttc
     5701 gatgaagtgg gtcatatcga ccaccagatc gcgttcaatc gggaagttag ctaacgcttc
     5761 aaccttcata ccgtcggtgt aatcacgcag gaaggtttta catgccagtt ttggcacgtt
     5821 gttaaccatc atgccgcagg aaccacaaat cgccatacgg caggaccagc ggtagctcag
     5881 gtccggtgcc aggttgtctt tgatgtagcc cagcgcatcc agtaatgagg tagttgcgtc
     5941 ataaggcact tcatagaatg cgctatgcgg tgcggtatcg acttccgggt tatagcgcac
     6001 cacctcaatt ttcaggtttt tcatctcagc cattcgcctt ctccttctta ttggctgctt
     6061 ccgccttatc ggctgcatcc gcttcgccac cgtaaacgcg tttagctggc ggcagcgtag
     6121 taatcttcac gtcgctgtac tccaggcgag tcgtgccatc agcatcgcgg aaggcgaggg
     6181 tgtgtttgag gaagttgacg tcgtcacgct cggtgcaacc ttcgtccaga cgctggtgtg
     6241 cgccgcggga ctctttacgt gccattgcgg agtgcgccat acattcagca acgttcagac
     6301 cgtggcccag ttcaatggtg tagagcaggt cggtgttgaa cacgctggaa gtgtcggtga
     6361 tgcgcacgcg tttgaagcgt tcctgcagct ctgccagctt gtcgatggtt ttctgcatca
     6421 gttccggcgt acggtagata ccgcagcctt cttccatagc caggcccatt tcgtcgcgga
     6481 tcttcgccca gttttcgccg ccatcctggt taaccagatc tttcaaacgt tgttcaacgc
     6541 cagctgcctg cgcttcaatt accgcttcgt tgccattacc ggcagttgct gcacgctctg
     6601 tcgcttgttc accggccaga cggccgaaga ccaccagttc cgccagggag ttagaaccca
     6661 gacggtttgc accgtgcaga ccaacagagg aacattcacc cacggcgaac agacctttaa
     6721 tgcgggtttc acagttctga tcggtttcga taccgcccat ggtgtagtgt gcggtcggac
     6781 gtaccggaat cggttcttta accggatcga cgccaacgta cgctttcgcc agttcgcaga
     6841 tgaacggcag acgttcatgc agttttttct cgccgaggtg acgcaagtcg agataaacca
     6901 catcgccacg cggcgtggag atagtgttgc ctttacgcca ttcgtgccag aaggcctgag
     6961 aaactttgtc gcgtggaccc agttccatat atttgttttt cggctcgccc agcggagttt
     7021 ccgggcccat gccgtaatct tgcagataac ggtagccatt tttgttgacc agaataccgc
     7081 cttcaccgcg gcagccttcg gtcatcagga taccggaacc tggcagaccg gttgggtgat
     7141 actgaacgaa ttccatgtca cgcagcggaa cgccgtggct tagcgccata cccataccgt
     7201 caccggtaac gatgccgcca ttggtattgt aacgataaac gcgacccgca ccgccggtag
     7261 ccataacgac cgcgttagca cggatctgca ccagcgtgcc ttccatcatg ttcattgcta
     7321 ccaggccgcg aacatgacca tcatcaacca gaatatccag tacgaaatgt tcgtcaaaac
     7381 gctggatctg cgggaattgc agagaggtct ggaacagcgt gtgcagcata tggaagccgg
     7441 tcttatcggc ggcgaaccag gtgcgctcga ttttcatgcc gccgaagcga cgtacgttga
     7501 cgctaccatc cgggcgacgg ctccatgggc atccccacag ttccagttgg gtcatttcgg
     7561 tggggcagtg gtggacgaaa taatccacga catcctgctc acacaaccag tcgccacccg
     7621 ctactgtatc gtgaaagtga tattcgaagc tgtcatgatc ctgcgcgaca gcggcggagc
     7681 ccccttctgc agcaacggta tggctacgca tcgggtatac ttttgagatt agtgcgattt
     7741 ttgcatttgg atttgcctgc gcggcagcaa ttgcagcacg taatcccgcg ccaccggcgc
     7801 ctacaatggc aagatcggct tgaaaggttt gcacgacatt cctccagatt gtttttatcc
     7861 cacagccccg tacttcaggg taagtgcctg aaagttacgg tctgcgaacg ctattccact
     7921 gctcctttat aggtacaaca gtatagtctg atggataagt ctgaaatttg acgagatcga
     7981 tttttttagt gcgcaagagg gctaaattat cactgaagat gattaattta attactaaac
     8041 catcagatcg tgcttttttt agtcactgcc cacccttgtt tgattcctgc gtaaaatttg
     8101 tctcgccgtc gcgttgcgag tagacttcgt gcccttgtca aaaactggag atttaactat
     8161 gagcgaaacg gcatcctggc agccgagcgc atccattcct aacttattaa aacgcgcggc
     8221 gattatggcg gagatccgtc gtttctttgc cgatcgtgga gtgctggagg tggagacgcc
     8281 ttgtatgagc caggcgacgg taaccgatat tcatttggtc ccgtttgaga cacgtttcgt
     8341 aggccccggg cattcgcagg ggatgaatct ctggttaatg accagcccgg aataccatat
     8401 gaaacgcctg ctggttgctg gttgtgggcc ggtattccag ctgtgccgca gcttccgtaa
     8461 tgaagagatg gggcgttatc acaaccctga gttcactatg cttgagtggt atcgaccgca
     8521 ctatgatatg taccggttga tgaacgaggt ggacgatctc ttgcaacagg tgctggactg
     8581 tccggcagca gaaagccttt cttatcaaca agctttcttg cgttatctgg aaattgaccc
     8641 actctctgcc gacaaaacgc aactgcggga agtggcagcg aaactggatt tgagcaacgt
     8701 tgccgatact gaagaagacc gcgacacgct tctacaattg ctgtttacct ttggcgtaga
     8761 gccaaatatt ggcaaagaaa aaccgacctt tgtgtaccac tttccagcca gccaggcatc
     8821 actggcgcaa atcagtaccg aagatcatcg ggtcgctgaa cgctttgagg tttattataa
     8881 aggtattgag ctggcgaatg gtttccatga attgacggat gcccgtgagc agcaacaacg
     8941 ctttgaacaa gataaccgta agcgcgcggc gcgcggtttg ccgcagcacc ccattgacca
     9001 gaatctgatt gaagccttga aagtcggtat gcctgactgt tccggcgtgg cattaggcgt
     9061 tgatcgtctg gtgatgttgg cgctgggcgc ggagacactg gctgaagtca tcgcctttag
     9121 cgttgaccgg gcataattct gaaaaattac tgaattaacc gcgaagaatg gcgtgataag
     9181 cgccattctg tagcatatgt tttcattttt ccgctaaagg ccacaattta agactggata
     9241 tttgatatca tccaggtatc aattctgtat tggtttttac gctgcccgct caatgttggg
     9301 cagtgcagtt cagtgggcgc gttttataca cgcgctgaaa tgaaggatgt tttcatgcct
     9361 cacacgataa aaaagatgag tctgatagga ctcatattga tgatctttac ttccgtattt
     9421 ggatttgcca atagcccatc ggcttattac ttaatgggtt atagtgcgat tcccttttat
     9481 atattttctg cattgttatt ctttattcca ttcgccttaa tgatggctga aatgggagct
     9541 gcttatcgca aagaagaagg cggtatctat tcctggatga ataatagtgt cggaccacgt
     9601 tttgccttca ttggtacgtt tatgtggttt tcctcttata tcatctggat ggtgagtacc
     9661 tccgcgaaag tttgggtacc gttctcaaca ttcctctatg gtagcgacat gacccagcac
     9721 tggcgtattg ccggactgga gcctacgcag gtggttggtc tgctggcagt ggcatggatg
     9781 attctggtca ccgtcgttgc ttcaaaaggg attaataaaa ttgcccgcat tactgcggtg
     9841 ggcggtattg cagtaatgtg tctgaattta gtattgctgt tagtaagcat tactattttg
     9901 ttattaaatg gtgggcattt cgcgcaggat attaatttcc ttgcatcacc gaacccaggt
     9961 tatcagtccg gtctggcaat gctatcgttt gtggtatttg ctatttttgc ctatggtgga
    10021 attgaagcgg ttggtggtct ggtcgataaa acggaaaatc cagaaaagaa ctttgccaaa
    10081 ggtattgttt ttgccgctat tgttatttca atcggttatt cgctggcaat atttttatgg
    10141 ggcgtcagca caaactggca gcaggtatta agtaatggtt ccgttaacct cggcaatatt
    10201 acctatgtgc tgatgaagag cctcggggtg acgctgggta acgcactgca tttgtcacct
    10261 gaagcgtcat tgtcgctggg cgtatggttt gcgcgtatta ccggactttc gatgttcctc
    10321 gcttataccg gtgcgttctt tacgctttgc tattcaccgc tgaaagccat catccagggg
    10381 acgccgaaag cgttgtggcc ggaaccgatg acgcgcctga atgcgatggg gatgccgtct
    10441 atcgccatgt ggatgcagtg cgggttggtt actgtcttca tcctgctggt ttcgtttggt
    10501 ggcggtaccg catcggcgtt ctttaacaag ctgacgctga tggcgaacgt gtctatgacg
    10561 cttccttacc tgttcctcgc gctggctttc ccattcttta aagcacgtca ggatctcgac
    10621 agaccgtttg tgattttcaa aacgcatatg tcggcaatga ttgcaacagt ggttgtcgta
    10681 ctggtggtga catttgcgaa cgtcttcacc atcattcaac ctgtggttga agctggagac
    10741 tgggacagca cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg
    10801 atttaccaga actattgcag tcgcatggcg aacaaacctg agttagctct cgactgatca
    10861 tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt gtctggaaag gaagacgtta
    10921 tggacgacgc tagtcgggat ccagtgataa ccgaagatga aatcagggag ctgcaatttt
    10981 ctgccgggga tgtggcggaa atagagcaaa cggtcctctc ttttgtcgat acccgccaca
    11041 cccgcaaagt agcgatggtg gtgggtaaca ctattaatac cctcaaagag cgcgatggtc
    11101 cgcggtgggg aaacctgccg gatatctatt gcgcttatct tattagatgt ctggtttttc
    11161 acggagaatt agtgggatac ggcgatttgt ttcgtatgcg ctatagcgaa attaagcgac
    11221 cgattatctc atgagtgcgc gcatgtttat cttgtgctgc atctggttta ttgtggcgtt
    11281 cctctggata accatcactt ccgcgctgga caaagagtgg atgattgatg gacgcgggat
    11341 taataacgtc tgcgatgtgc tgatgtatct cgaagatgac gatacccgtg atgtcggtgt
    11401 gataatgacg ttaccgctct ttttcccttt tctctggttc gctttgtggc gaaaaaaacg
    11461 cggctggttt atgtacgcca ccgcgctggc tattttcggc tattggctgt ggcagttttt
    11521 tctgcgctat cagttttgct tataagccgg atgggttcat ccggccacaa agaccttaca
    11581 aacttcccgc ctgacgacct ttgcccgcag aagttaatgt tctccccgtt tgcttaccat
    11641 tcaggctttc caaacgcatc tggaagggcg ggaatggcat atcaataccg tgggcatgga
    11701 atcctgccag aattagctgg tgaatctcat ggcgtagtgg catacggtga cccatctcag
    11761 cggcgtaaat acgcagctcg aaaatttgaa tcccctgttg cagatccacc aggaagactt
    11821 ccggtgctgg gttatcgatt accagcgagc agcgacgcgc tgcggtgagc aggatttccg
    11881 tcacttcttc actgttggca tcggcagggg ccggaatcgt caacaccacg cgcgtgaccg
    11941 agtcagagag cgaccagttg ataaactgct cggtaataaa cgccttgttc ggcacgataa
    12001 tttctttgcg gtcccagtcg ctgatggtgg tggcgcgggt gttaattttc gtcacactac
    12061 cggtaagatc gcggatcgtt accgtatcgc caatgcgaat cggtttttcg aacaggataa
    12121 tcaggccaga gataaagttg gcgaaaattt cctgcaaacc aaaaccgaga ccaacaccga
    12181 gcgcggcgac cagccactgc aatttcgacc actcaatacc aatcatcgag aagccgacca
    12241 gcccgccaat cagcatcagc agatatttgg tgatggtggt gatggcgtag cccgtacccg
    12301 gcgttaaatc caggtgctgc agaatcgcca gttccagcag cgcgggcagg ttgcgcacca
    12361 gctgcgtggt gatgataaac accagaatgg caatcagcac cgcaccgagg gtaatcggct
    12421 ccaggctttc tacgccctgt accgtggaag tgacatccca cagcgaaata ttttcgagga
    12481 agccgaaagc ggaatggatt tctgaccaca gcacaatgac cgaaagcagg gcgatcaaca
    12541 tcaaaattga gcgcaccagc cgcaaggatt gcgcactgat ggcatcgaga tcgacttcgc
    12601 tttcatcgac ttcaattgct ccttccgggc tactgtgatg atgcgcttcc tcttcgccac
    12661 gcgcacgctg cgctaacatc tctgcccggc gatgcttcgc ccggtcaaac gccagcctgc
    12721 ggcgctggat cagcatccag cggcggataa cgtgataaac caccagtagc aggaaccaga
    12781 tggcaaccga ggtttcaagc ctcgctaaca gcgcctgcgc cgttgccaga taacccaccg
    12841 ccgacgccag aatggcaacc agcggtgcgc caatcatcat gttccacagc atatggttgg
    12901 taatgttgtc gccgctgcct tctttgttga gatacagcgg gatcccggcc tttttcagac
    12961 tgagagtgac caccgccagc gcaccgcaaa tgaggataaa gcaaagccgt cccagcgaac
    13021 cggagaattc acggtcgtcg aggttatcga acatcatcag cgccataatc agcggcacaa
    13081 taagcccgat gctcatcagg tagtagcgca tcccacggga aacccgttcg cgcggccagc
    13141 caaaatgagc gataaacaag ccgttcgggc gggcaaaggt ggcgcaaatc atcactaccc
    13201 acagcagcgg cacggtggct gttacgccat caccaatcgc gaccgccagc ggataaggcc
    13261 acgcctcgcg caagccgtaa cccagcgtca tccacagcac aggcagcggt gacgcgacga
    13321 gaatcgacca gaaaagagtg cgtaacgtca gccagaagtg atcctgagtc actttgccga
    13381 ctttcgccgc cgaacgttca agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac
    13441 cgaccagaat caacgcgcca agcagcggca aaatcgtctc tttactggtc agcatcatta
    13501 cactggcttt gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg
    13561 cgatttccag cggccaggcg atggtcatcg ggcgcacgtc agaggtccag aacagatagc
    13621 ggtgcgtcgc ttcattcacc tctttcagcg catcctccag ttgcccgttg gagactttca
    13681 gcttggtcag ttccagtagt agcgtgtctc caccctgcaa caatgagttc agcaactcac
    13741 gctgagtgcg tagctgtgct tccagaatac ggttttgctc ggcggtcagc ggctgaccgt
    13801 cggcctgatg gatttgccgt agcagcggct gtttatttag cagatcctca taacgtaacc
    13861 gttgcacacg caactgcgcc atttcggtat caagctgttg tggttttggc atttccggta
    13921 gccgtgccac ctgcgcccgc aacgcttcgc cgagcaggtt ggacgagccc agccattgcg
    13981 actgttcacg cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac
    14041 gctgttgcga ggcaacgaga tccatccgct gcgcctgttg attcagcgct gccgatagtt
    14101 cgcggttaat tttgaattgc gcgacgatat ctttcggcaa atcggcactg ttttctgcca
    14161 gtaattcggt actttccagc gcccgctccg cctcaagctg acgttggctg ttcaactgat
    14221 tacgtaaggc ctgcaaatac gcatccagtt gctggctctc tttttccgcc agctctgagc
    14281 gtaagcgcgc taattcctga cggttattgg cagacagctg cgccagttcc agttcatcga
    14341 cgagcgcttt caggcgtgcg gagtcagact gcaacgcgaa attttgtgcc tgattgagcg
    14401 gggaattgcc ggtaagcgtc cccaggcggc gctcgatttc atttaactga cggcgggcat
    14461 cggtttgctg ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg cgttcctgct
    14521 cttgctgggc ctgacggctt ttatccagca actggctgct gacctggaga atttcctgat
    14581 tcagcgcgtc ggtagacatt cccggtgaca cgctgcgcgg ttcgtcacgc atgttgttta
    14641 attgtgcgcg cagagtagcg gaaagtttcg ggtaattatc aatgacttct tgatattgct
    14701 tgatgcgctc aagggaacct tttcgttcct caagcgcatt taaggcggac tggagcgcct
    14761 ctacgacttc cggctgcgcg ggtttcgccg cttttgcctg ctccagttct tgtgagattt
    14821 gtttgctatc gggggccgtc gcggcgtacg ccccccaact gaggcaccag gccatcagaa
    14881 aagtgataat caggcgcacg tcagcgtttc ctttgatgga ttagacctgg tctttgttgt
    14941 cgtcaaccaa tgggttggcg tcgtgttctg cttcgatctc ttcagcagga agcggggccg
    15001 gttcagcgtc tggtgtaaca aaggtttcgg tagatactgc cagcggctgg ccaattttcg
    15061 tgacagacag gctttccagt tgctcaacca gattcacttt acccggtgca aacaggttga
    15121 taacggtgga accgagttta aagcgaccca tttcctggcc tttcagcagt gccacagaac
    15181 cgtcgttttc cccggcaggc caggtccagc gcttgatgat accttcgcgc ggcggcgtaa
    15241 tggtgcccgc ccagaccgtc tcaatgctgc ctacaatcgt cgccccgacc agaatctgcg
    15301 ccattgggcc aaattcggta tcgaacaggc agatcacgcg ttcgttacgg gcaaacagat
    15361 tcggcacgtt ctgagcggtg agatggttaa cggaaaagag atcgcccggc acgtagatca
    15421 tctcacgcag aataccgttg cacggcatgt gtacgcggtg gtagtcacgt ggggagaggt
    15481 aagtggtcac aaacgtaccg ttgcggaaca ggtccgccat cagatagttg ccagccagca
    15541 gggcttcgag gctgtagttg tggcctttgg cttgcaggat tttatcttct tcgattttac
    15601 ccaactggct gataacgcca tcggcaggca taaccagtac attcggatcg gtatcgattg
    15661 ggcgtacttc gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg
    15721 tgtccggctt ttgcgcctct ttcatatcga ccttgtagta tttaacgaac agatcgataa
    15781 ccagttttgt cagccatcct gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc
    15841 atagtttcgg cagaatgtac tgtagcgaaa gtttaaatga atttaacaag gtagcctcca
    15901 ggccattgtt ttgtcgttcc tgatccggcc tacatgccgg atcctgaaaa aaaggggacg
    15961 attctaacga cggttagctt aattgtcagt catccgtatc agaaaagttt ttacgcgttt
    16021 ttacctgcgc catgctttcc agaatacggt gatagttttc gaaacgggtt tccgcgattt
    16081 ttccttcctc aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc
    16141 gatatttaca cagacctaag tagtcatgga attcgacaaa gccctgagtg atttgttccg
    16201 gctccaggtg ccagaggccg aactcacgca ctcctgggga gtcaatcaca tcaccgccgt
    16261 gcgggaagtg atacagccga gcggcggtcg tggtgtgctg accgagaccc gagttgtcag
    16321 agacatcgtt ggtcaggatc tctttttgca gacccagcaa tgcgttcagc aggctggatt
    16381 tgccaacgcc agactgcccg gcaaaaatgc tgatgcgccc ggtcaacgcc tcttccagcg
    16441 gtttcagccc atcctgagta tgactggaaa ccatcaatac gcgataaccg atattgcggt
    16501 agatatccat ctgctcgttg acgaatgcca tgccttcgtc gtccagcagg tctatcttgt
    16561 tgagcacaat aatcggctca atctgcaagg tttcgcaggc caccaggtaa cggtcgataa
    16621 tattgagcga cagctccggc aaaatggcgg agacaatgac aatctggtcg atgttggcag
    16681 caataggttt cacaccgtcg taaaaatccg ggcgcgtcaa cactgaggtg cgctcatgca
    16741 ccgcttccac gatccctttg acatttacgc cttccgccgc tggtttaccc ggacgccaga
    16801 ctacgcggtc gccggtcacc aacgaacgga tggtacggcg aatattgcag cggtgaacgt
    16861 cgccatcggc ggattccaca tcagcgtgca taccaaagcg gctgatgacg ataccttcat
    16921 caggctcgcc aaacagattg tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac
    16981 gctggtgatt ggcgttcacg cggcgctgct ggcctttgga gagtttattt ttactcaatc
    17041 gtacagactc ctggtcgccc ctgatgggca aaacatctat gatacacgca attgtggatc
    17101 aatatagtca ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac aacctgattt
    17161 ggatcgatct tgagatgacc ggtctggatc ccgagcgcga tcgcattatt gagattgcca
    17221 cgctggtgac cgatgccaac ctgaatattc tggcagaagg gccgaccatt gcagtacacc
    17281 agtctgatga acagctggcg ctgatggatg actggaacgt gcgcacccat accgccagcg
    17341 ggctggtgga gcgcgtgaaa gcgagcacga tgggcgatcg tgaagctgaa ctggcaacgc
    17401 tcgaattctt aaaacagtgg gtgcctgcgg gaaaatcgcc gatttgcggt aacagcatcg
    17461 gccaggatcg tcgcttcctg tttaaatata tgccggagct ggaagcctac ttccactacc
    17521 gttatctcga tgtcagcacc ctgaaagagc tggcgcgccg ctggaagccg gaaattcttg
    17581 atggttttac caagcagggg acgcatcagg cgatggatga tatccgtgag tcggtggcag
    17641 aactggctta ctaccgcgag cattttatca agctgtaaaa tttttcctgg tcacgtaagc
    17701 gccggaatcc acttgcggcg gcgtgatttt gacgctaaat tgaacgtctt gctgataatt
    17761 tcagcacttg agataaaaac tcaaaaaaaa ttttttaggg gggttgcaga gggaaagatt
    17821 tctcgtataa tgcgcctccc gtaacgacgc agaaatgcga aaattacgaa agcaaaatta
    17881 agtagtacgc gggaatagct cagttggtag agcacgacct tgccaaggtc ggggtcgcga
    17941 gttcgagtct cgtttcccgc tccaaaattt gaaaagtgct gcaaagcaca gaccacccaa
    18001 tcgcagtatt tcgaattgcg acaaggcggc aactgaacga atccttggga acttacaaaa
    18061 gtaagtgact aaggtgagtg aaggcagcca aggcagtagc ggttcgaaag acgaagatta
    18121 agcgggaata gctcagttgg tagagcacga ccttgccaag gtcggggtcg cgag
//
LOCUS       DAZGTJ010000094        18099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000094 DAZGTJ010000000
VERSION     DAZGTJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.094"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            199..765
                     /locus_tag="ACHK8E_004030"
     CDS             199..765
                     /locus_tag="ACHK8E_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0346232.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
     gene            851..3490
                     /locus_tag="ACHK8E_004031"
     CDS             851..3490
                     /locus_tag="ACHK8E_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0346233.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3510..4259
                     /locus_tag="ACHK8E_004032"
     CDS             3510..4259
                     /locus_tag="ACHK8E_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0346234.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            4276..4782
                     /locus_tag="ACHK8E_004033"
     CDS             4276..4782
                     /locus_tag="ACHK8E_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0346235.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            4779..5249
                     /locus_tag="ACHK8E_004034"
     CDS             4779..5249
                     /locus_tag="ACHK8E_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0346236.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5246..5770
                     /locus_tag="ACHK8E_004035"
     CDS             5246..5770
                     /locus_tag="ACHK8E_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0346237.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            5772..6614
                     /locus_tag="ACHK8E_004036"
     CDS             5772..6614
                     /locus_tag="ACHK8E_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP0346238.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(6730..7281)
                     /gene="smrB"
                     /locus_tag="ACHK8E_004037"
     CDS             complement(6730..7281)
                     /gene="smrB"
                     /locus_tag="ACHK8E_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP0346239.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7447..8379
                     /gene="prmB"
                     /locus_tag="ACHK8E_004038"
     CDS             7447..8379
                     /gene="prmB"
                     /locus_tag="ACHK8E_004038"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0346240.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8414..9499
                     /gene="aroC"
                     /locus_tag="ACHK8E_004039"
     CDS             8414..9499
                     /gene="aroC"
                     /locus_tag="ACHK8E_004039"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP0346241.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9502..10326
                     /gene="mepA"
                     /locus_tag="ACHK8E_004040"
     CDS             9502..10326
                     /gene="mepA"
                     /locus_tag="ACHK8E_004040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP0346242.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10326..11135
                     /locus_tag="ACHK8E_004041"
     CDS             10326..11135
                     /locus_tag="ACHK8E_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0346243.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11135..11683
                     /gene="epmC"
                     /locus_tag="ACHK8E_004042"
     CDS             11135..11683
                     /gene="epmC"
                     /locus_tag="ACHK8E_004042"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP0346244.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11717..11995
                     /locus_tag="ACHK8E_004043"
     CDS             11717..11995
                     /locus_tag="ACHK8E_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP0346245.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(12051..14057)
                     /gene="mnmC"
                     /locus_tag="ACHK8E_004044"
     CDS             complement(12051..14057)
                     /gene="mnmC"
                     /locus_tag="ACHK8E_004044"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP0346246.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14216..15436
                     /gene="fabB"
                     /locus_tag="ACHK8E_004045"
     CDS             14216..15436
                     /gene="fabB"
                     /locus_tag="ACHK8E_004045"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP0346247.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15701..16879
                     /locus_tag="ACHK8E_004046"
     CDS             15701..16879
                     /locus_tag="ACHK8E_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346248.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16876..17871)
                     /gene="flk"
                     /locus_tag="ACHK8E_004047"
     CDS             complement(16876..17871)
                     /gene="flk"
                     /locus_tag="ACHK8E_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP0346249.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
BASE COUNT         4341 a         4523 c         4781 g         4454 t
ORIGIN      
        1 aaaatctaaa ttgcgtcagt catattaaaa tatcaatatg ttcggcacgc caaagaatat
       61 tatatcaagg aacctgtttt ttcaggagct gaaaatctgt tcattggctg cttaaaagtc
      121 gctgcaaaaa ttgaagaagt tctgtgtcaa tatttcagtt ggcttatatt ctaattattt
      181 atgatttaag gacaaataat gagtaagttt gtaaaagcag cagttgccac ggcaatgctc
      241 atgggggcgc tgacttcagc atcagtcgtt gccgcaggta acaacggtac agtgagattt
      301 tacggcacaa ttgaagactc tccgtgctcg attgttcctg acgatcataa actggaagtg
      361 gatatgggca gcattggtac aggttcttta accggtggca agactaccac acctaaagat
      421 ttccagattc gtttacagga ttgtaatttc aatactgaaa cgacaatgac cacgacattc
      481 actggtaatc cgtactccgg caactcaaaa aactattccc tgtctaacat ggataatgga
      541 actgaaattc caaatgtaag tctggtgatt ggcgaccaac gtggtactgg ttatgacctg
      601 ggggcaggca tcgatcagga catcgcgatg gacagttcaa caggaaaagg caaacagaaa
      661 caaacgttga actttaaagc ctggttagta ggtgaaactg cagcggtaac accaacccca
      721 gcgccgtttg aaacgctaac gacgttccag attacatatc tctaattatg ctgttccccc
      781 ataactgcaa tgttatgggg tttataaata aaagataact aaattataat aatcatggat
      841 ggatacgtag atgcctgaac attcttttct tcgtttacgg gggctttcat ggtggattgc
      901 tctggcaatt tccgggagtc cttttaatgt actggcagat gataccattc aatttgatgc
      961 tcgatttctg gagttaaaag gaaataccaa aattgatctg gggcgttttt ctcaaaaagg
     1021 gtatgttgaa ccagggaaat acaatttacg tgttcatgta aataatcacc cattacctga
     1081 tgaatacgat atctattggt atgtagctga aaacgatccg aataaatcat atgcctgctt
     1141 accacctgaa ctgatagccc agtttggctt caaagatgat tttgcgaaga gtttgcaatg
     1201 gggtcatgat ggacaatgtc tgaaaactga ccaaatagga ggtatggaaa ttaagggcga
     1261 tttaagtcaa tctgcgctct tagtttctgt gccacaggca tatctcgaat acactgacga
     1321 tgactgggac ccaccttcgc gttgggatga ggggattcct gggcttattg ccgattacag
     1381 tatcaacgca caaacccgac atgaaaatgg gggggatgac actaacgaca tcagcggtaa
     1441 tggtacggtc ggcgttaacg tggggccgtg gcgcttacga gccgactggc agagcgatta
     1501 ccaacatacc cgcagtaacg atgacggcga cactgatgat agcggtacgc aaaaaaactg
     1561 ggagtggagc cgttactacg cctggcgcgc cttgccgtca ttaaaagcca aactttcgct
     1621 gggtgaagat tatctcaact ccgatatttt tgatggcttc agttacattg gcggcagcat
     1681 cagtaccgat gatcaaatgt taccgccaaa catgcgcggc tacgcacccg atatttcagg
     1741 tgttgcacgc actaccgcta aagtgaccgt aacccaaatg ggccgtgtca tttacgaaac
     1801 ccaagtgccc gcaggtccat tccgcattca ggatattggc gattccgtat ctggcacgct
     1861 gcatgtccgc attgaagaac aaaacggtca ggtacaggaa tatgacgtca gcacggcgtc
     1921 catgccgttt ctgacccgcc ccggtcaggt gcgttacaaa gtgacaatgg ggcgtccaca
     1981 aaactgggac catcaggttg caggcagctt cttctcgggc ggtgaggcgt catgggggat
     2041 tgcgaatggc tggtcgctct acggcggggc gttggccgat gaaaactacc agtcggcagc
     2101 gttaggcctc ggtcgtgacc tcgcgctgtt cggagcgcta gcgtttgatg tcactcactc
     2161 tcgtgttcaa ctcgacgata acagcgtgta tggcaacaaa acgctggacg gcaactctta
     2221 tcgcgtcagc tatgccaaag atttcgatga actcaacagt cgggtgacgt ttgccggata
     2281 tcgcttctct gaaaaaaact acatgaccat gagtgagtat ctggatgcga acgacaccga
     2341 ccgtgcgcgc acgggtaacg acaaggaaat gtacacggtc acttacaacc agaactttag
     2401 ggatgcgcgc gtttcggtct atctcaatta ttctcaccat acctattggg atcgcgagga
     2461 tcagaccaac tacaacatga tgctttctca ttatttcaat ttaggaagtc ttcgcaacct
     2521 gagcgtttct ctcaccggtt atcgctacga atatgataaa agtgccgaca aaggtgttta
     2581 tctgtccttg agtttgccat ggggcgacaa cagcaccatc agttacaacg gcaattatgg
     2641 cagtggtgcc gacagcagcc aggcgagttt gtatcaccgc atcgatgacg ctagccacta
     2701 tacgttgagc gccggaacca gtgaaaatca caccagtctt gatggttatt acagtcatga
     2761 cagtacgctg gcaaaagttg acctgagtgc aaactaccat gaggggcaat acacatctgc
     2821 gggggtctcc ttacagggcg gtgtaacact gactgctcac ggcggggcgt tgcatcgtac
     2881 gcaaaatatg ggaggcacac gtctgctaat cgacgccgat ggcgttgcta atgtgccggt
     2941 cgaaggtaat ggttccgccg tttataccaa catgttcggg aaagcggtgg tggcagatgt
     3001 taatgattac taccgtaacc aggcttatat cgatttgaac aaactaccgg aaaacgccga
     3061 agctacccaa tcggtggtcc aggcaacgct gacggaaggc gctattggct accgtaaatt
     3121 tgcggtgatc agcggtgaga aggcgatggt agtgctgcgc ttgcaggatg gcagccatcc
     3181 gccatttggg gcggaagtga aaaacgataa tcaacaacaa gttgggctgg ttgatgacga
     3241 aggtaatgtt tatcttgcag gcgttaagcc gggtgaacat atgaccgttt tctgggaggg
     3301 agaatctcac tgcgatatca gcctgccaga tccgttaccg aatgacctgt tcaatggctt
     3361 gttgttgcca tgccagcaaa aagggggaag tgctcccgtc ataacgcatg atattcaacc
     3421 tgtaattcag gagcagacac aacaggtgac accaatggaa ccgccgatgt ctgtttcatc
     3481 gaaccaataa cgtgattaag gaatgatcca tgactaaaca tctctctgtc aaaacattgg
     3541 caacggtact gtcgttactg gctgcaacgg taagtttttc tgctctggcg tcagtaacgc
     3601 ccgatcgtac tcgcctgatt tttaatgaaa gcgataaatc catcagcgtc acgctgcgta
     3661 ataacgaccc gaaacagcct tatctggcac aaagctggat cgaagatgaa aagggcgtca
     3721 agattagttc gccgctggca gtattgcccc ccgtacagcg cattgatgca atgatgaatg
     3781 gtcaggtgaa aattcaggga ttgccagata tccataaatt gcctgccgat cgtgaatcga
     3841 tattttattt caatgtgcgt gaaattccgc cgaaatcgaa caaagccaat acgttacaaa
     3901 ttgcgctgca gacacgcatc aaacttttct ggcgacctaa agccctggaa aaagctagca
     3961 tgcaaaaccc atggcagcat aaagtgacct taacccgtaa cggtcaggaa tttaccgtca
     4021 ataaccctac tccgtattac gtcattttca gtaatgccag cgcgcaaaaa aatggcaatc
     4081 cggcagacgg tttctcgccg atagtgatgt cgccaaaaac ctccgcaccg ctcaaggtga
     4141 agatggctag cgtgccggtg ctgacctatg tcaacgacta tggcgcacgg atgccactct
     4201 ttttcacctg tagcggcaac agttgtcagg ttgatgaaga gctgagccgc aaaggttaat
     4261 gacggggaag taagtatgaa aagatcttgt tcagtctggg cgggaacatg gttactgatg
     4321 tgttgtattg gtagcgactt tgcagttgca gggagtaaat cagtaaattt gagtttacga
     4381 gtgctcgttg atgcccctcc tccttgcacg gttaatggtg cgacggttga gtttggtaat
     4441 gtattcatca ataagatcga tggggtaggt tataaacaac ccattgatta ctcgcttgtt
     4501 tgtaacaacc tggcaatgga tgacctgcgt ttacagatgc gggcaacaac ggttgtgatc
     4561 aacggtgaaa cagttatcag cacaggtatt tcagggtttg gtattcgggt tcagaaaagt
     4621 agtgatcact caacactcga cttaacgtct ggttcatggt tgccgtttaa tttttcctcg
     4681 ggcgtccctg cactcgaagc agtgccggtg aaacagagtg gtacaacctt atcggcagcg
     4741 gaatttaacg ccagtgccac aattgtggtg gattaccaat gaaacggatc tcacttctta
     4801 tgctgtgggg ctttagctcg atggcactct caaatgtctc ttttcacggc tatttagtgc
     4861 agccacctaa ctgcactatt agcaacggta aaaccattga aataaccttt caggatgttt
     4921 ttgttgatga cattaatggc agtaactatg agcaaaccgt gccgtattcc attacctgtg
     4981 atacggcggt tcgcgaccca tcaatggaaa tgaaattgag ctggagcggt acgcagagcg
     5041 actttgacga tgccgcggtt agcaccgata tctctggcct ggcgattcgg cttaaacagg
     5101 cagggcagag ctttaagctc aatacggcac tggtggtgaa tgaaacggcc ttaccggtgt
     5161 taaccgcagt accggtcaaa aagagcggtg tcgatttgcc tgaggcagac tttgaagcct
     5221 gggccacgtt acaggtggat tatcaatgaa gaaaaagcgt gcgttggttt ttatctgtag
     5281 cctgatgctg ttgtacagcg ttactgcccc ggcggtcgac aacctgcact ttaccggtaa
     5341 tttgctcggt aaatcctgta cgccagtgat caacggtagc ctgttggcgg aggtgcactt
     5401 tcccaccatt gccgccagcg atttaatgaa cctcgggcag tcagagcgag taccgttggt
     5461 atttcaattg aaggattgca aaagctccac gctgtttagc gtcagggtaa cgctgacggg
     5521 aacggaagat agcgaactgc ccggatttct ggcctttgac gcctcatctt ctgcatccgg
     5581 agctgggatt ggtattgaaa cggcggcagg cgcggcggtg ccagtcaaca gcacaacggg
     5641 tgtcacgttc ccgctaaacc agggcaataa cagtctcaat tttaatgcct ggctacaggc
     5701 gaagggcggg cgggatgtga cgtccggtga tttcagcgct acggcgacgg caacctttga
     5761 gtatttttaa catgaaaaaa tggacaatga tttttgcgag cctggtgttg ctggtgatga
     5821 gtgcgagcgt tccaaagagc tatgccgggg ataaactcac cagcgcgagt ttcgacagtg
     5881 tgaagattta ttatgggaag gggaaaatgt ctattcataa caccggaggt ttgtatgtca
     5941 cggtgttaac tcccaacgag gcggcctatg gggtgtacaa cgatgccata aggaagaacg
     6001 agcaagccag cgttatttcg tggacggggt cgggaccagc gccagtgttg atcttaactg
     6061 actacggtaa tgctgatagc tcgaattgtc ctgggataaa tacggcgaca tttcagtgtg
     6121 catatgagac gtttaagata accgttcagt ccgacagtta tggttgcccg tggctcgctt
     6181 ctttttatac gatcacagat caacccggat tcgggagata tactgggcca acgaaccatg
     6241 ccacagtatg tccgacgatc cctgtcgcgt cgtatgacat ctcgtggagt gagaattacg
     6301 tcagccacaa taaggcgtta cagttgcaat ccaatggctc gacaatcacc accacgcttt
     6361 cgacctacct gatggaagat ggcaaactct gtgatggcag cgtttttgat agcagaggtg
     6421 catactgccg cgtggtctcg gaactgttaa cctttacctc ctacggttgc gataaatcaa
     6481 cggtaacggt ttctcccaca cggcatccgg taaccgataa acagctacat gatatcgtgg
     6541 taaatgtgaa caccagtagc cgacaaccga ttgattctac gtgccgattc cagtatgtac
     6601 tgaatgaatt gtagtgacga tattttcaca agtcgacggg gccatctggc cctgtcgtca
     6661 tttccggcgt ctgttttatg atatgatggc taaggcgaac tttccctcta cagcattcga
     6721 tgccagcatt cagggcaatt caggcggcag ccactcttcc acttcaatca acaccaacaa
     6781 agccgcatcg ccaccatact cttttggtgc ctggtgaaat gccattacat gtggatgctg
     6841 cgccagccac agcggcgttt gctgcttcaa aatatgcttc ccgtgaccat gcatcacgca
     6901 ggcacaaaac acatgttcac ggcggcaggc ggcaatcaac gcccccagtt cctgtttggc
     6961 ttgcaactgc gtcagaccgt gcaaatcaag aaacaactcc ggtgaataat caccgcgccg
     7021 cagtttcttc gcttcaaaat ggctgacatc cgggcgaaca tatttcaccg gaccttcggt
     7081 atttaataat ggctgaaact catcggaaaa ataatggctg gcatcggcct gctcctgaat
     7141 caaccgcttg accggtactt cgctgatttt tttacgctgc ggtcggtgga caatcgtgtc
     7201 ctgcttaatc ttgcgagtcc ccgccatcaa ctggcgaaac agcgcctggt cctcctcgct
     7261 aagtgttgtt ttctttttca tttaactgtc tctatttcta attttcctac agtttacctg
     7321 actcgtagca caatcgatcg ttttaaacgt atttttcctc actcaccgcg cgtgaatgct
     7381 gatttatcgt catcttcatg gcaaactagc cgccgaaatt attgcgatca tgccctggag
     7441 gaatacgtgg ataaaatttt cgttgatgaa gcagtaaatg agctgcaaac cattcaggac
     7501 atgttgcgct ggtcggtgag ccgcttcagc gcggcgaata tctggtacgg ccacggcacc
     7561 gataacccgt gggatgaagc cgtgcaactg gtgttgcctt cactctacct gccgctggat
     7621 attccggaag atatgcgcac cgcgcgtctg acttccagcg aaaaacaccg tattgttgag
     7681 cgcgtgatcc gccgcgtcaa cgaacgcatt ccggtggctt acctgaccaa taaagcctgg
     7741 ttctgtggcc atgaatttta cgtcgatgaa cgcgtgctgg tgccgcgctc gccgattggt
     7801 gaactgatca acaataaatt tgccggactc atcagcaagc aaccgcagca cattttagat
     7861 atgtgtaccg gtagcggctg catcgccatt gcctgtgctt atgccttccc ggaagcggaa
     7921 gtcgacgcgg tagacatctc tcccgacgcg ctggcggttg ctgaacagaa catcgaagaa
     7981 cacggtttga tccacaacgt cattccgatt cgttccgact tgttccgtga tttgccgaaa
     8041 gtgcagtacg acctgattgt caccaacccg ccgtatgtcg atgcggaaga tatgtccgac
     8101 ctgccaaacg aataccgcca cgagccggaa ctcggtctgg catctggcac tgacggcctg
     8161 aaactgacgc gtcgcattct tggtaacgcg gcagattacc tcgctgatga tggcgtgctg
     8221 atttgtgaag tgggcaacag catggtacat cttatggaac aatatccgga tgttccgttc
     8281 acctggctgg agtttgataa cggcggcgat ggtgtgttta tgctcactaa agagcagctt
     8341 attgccgcac gagaacattt cgcgatttat aaagattaag taaacacgca aacacaacaa
     8401 taacggagcc gtgatggctg gaaacacaat tggacaactc tttcgcgtaa ccaccttcgg
     8461 cgaatcgcac gggctggcgc tcggctgcat cgtcgatggt gttccgccag gcattccgct
     8521 gacggaagcg gacttgcaac atgaccttga tcgtcgtcga cctgggacat cgcgctatac
     8581 cacccagcgc cgcgagccgg atcaggtcaa aattctctcc ggtgtttttg aaggtgttac
     8641 tactggcacc agcatcggat tgttgatcga aaataccgac cagcgttctc aggattatag
     8701 tgcgattaag gacgttttcc gtccaggtca tgccgattac acctatgaac aaaaatacgg
     8761 cctgcgcgat tatcgcggcg gtggacgttc ttccgcccgc gaaaccgcta tgcgcgttgc
     8821 agcaggggcg attgccaaaa aatatctcgc cgagaaattt ggcattgaga ttcgcggctg
     8881 cctgacccag atgggtgata ttccgctgga aatcaaagac tggtcgcagg tcgagcaaaa
     8941 tccgtttttc tgcccggacc cggacaaaat cgacgcgtta gatgaactga tgcgcgcgct
     9001 gaaaaaagag ggtgactcca tcggcgcgaa agtcaccgtc gttgccagtg gcgtacccgc
     9061 cggacttggc gagccggttt ttgaccgcct ggatgccgac atcgcccatg cgctaatgag
     9121 cattaacgcg gtgaaaggtg tggaaattgg tgatggtttt gacgtggtag cgctgcgtgg
     9181 cagccagaac cgtgacgaaa tcaccaaaga cggtttccag agcaaccatg cgggcggcat
     9241 tcttggtggt atcagcagcg ggcagcaaat cattgcccat atggcgctga aaccgacctc
     9301 aagcattact gtgccggggc gcacgattaa ccgctttggc gaagaagttg agatgatcac
     9361 caaaggccgt cacgatccgt gtgttgggat ccgcgcggtg ccgatcgctg aagcgatgct
     9421 ggcgatcgtt ttaatggatc acctgttacg gcaacgggcg caaaatgctg atgtgaagac
     9481 tgatattcca cgctggtaaa aatgaacaaa actgcgattg tgctgctggc cctgcttgcc
     9541 agtagcgcca gcctggcagc gacgccgtgg caaaaaataa cccaacctgt gccgggtagt
     9601 gcacaatcga taggcagttt ttcaaatggc tgtatcgtcg gtgctgacac gctgccgata
     9661 cagtccgaac attatcaggt catgcgtacc gatcagcgtc gctatttcgg acacccggat
     9721 ctggtgatgt ttatccagcg tctgagtcgc caggtgagca atctgggtat gggtacggtg
     9781 ctgattggcg atatggggat gcctgctggt ggtcgtttca acggcggtca cgccagccac
     9841 cagactggac tggatgtcga tatcttcctg caactgccaa aaacacgctg gacctccgcg
     9901 cagctcttgc gcccgcaagc gttagattta gtttcccgcg acggtaaaca cgttgtcccg
     9961 acgctgtgga agccggaaat tttcagcttg attaaactcg ccgcgcagga caaagacgtc
    10021 acgcgcattt ttgttaatcc ggcgattaaa caacaacttt gccttgatgc gggcaccgat
    10081 cgcgactggt tacgcaaagt gcggccctgg ttccagcatc gcgcccatat gcatgtacga
    10141 ctacgttgtc ctgccgatag cctggagtgt gaagatcaac ctttaccgcc accgggcgat
    10201 ggttgcgggg cagaactgca aagctggttt gaaccaccaa aaccgggaac aacaaagcct
    10261 gagaagaaga caccgcctcc gttgccgcct tcctgccagg cgctactgga tgagcacgtg
    10321 atctaatgga aacgtttaat agcctgttta tggtttcccc gctgctgctg ggagttctct
    10381 tttttgtcgc catgctggcg ggatttatcg actcgattgc cggtggcggt gggttactca
    10441 ccattccggc attgatggcg gcggggatgt ctccggctaa cgcgctggca accaataaac
    10501 tgcaagcctg cggcggctct atttccgcca ctatctactt tattcgccgc aaagtggtta
    10561 gtttaagcga tcagaaactc aatatcgcca tgacctttgt cggctcaatg agcggcgcat
    10621 tactggtgca gtatgttcag gctgatgtct tacggcagat tctgcccatt ctggtgattt
    10681 gtatcggcct ctatttcttg ctgatgccaa agctgggtga agaagatcgc cagcgccgga
    10741 tgtatggact gccatttgcg ctggtggcgg ggggctgcgt cggtttttac gatggattct
    10801 ttgggccagc tgccggatcg ttttacgcgc tggccttcgt tacgctgtgc ggattcaacc
    10861 tcgccaaagc cacggctcac gccaaattac tcaacgcaac gtcaaacatt ggcggtctgc
    10921 tgctgtttat tctcggcggc aaagtgattt gggcgacggg ctttgtgatg ctggtggggc
    10981 agttcctcgg cgcgcgcatg ggttcaagac tggtgttgag caaaggacaa aagctgatcc
    11041 gcccgatgat cgttatcgtc tcggcggtga tgagtgcaaa attactttac gatagccacg
    11101 gacaggagat cctccactgg ttggggatga actaatgaac agtacacacc actatgagca
    11161 gttgattgag atttttaata gctgctttgc cgatgaattt aatacccgtc tgattaaagg
    11221 cgacgacgaa ccgatctatc ttcctgctga tgcggaagta ccgtataacc gaatcgtctt
    11281 tgctcatggc ttttatgcca gcgcaattca tgagatttcg cactggtgta ttgccggaaa
    11341 agcgcgccgt gagcaggttg atttcggcta ctggtattgc ccggacggtc gcgatgccca
    11401 gacgcaaagc cagtttgaag atgttgaagt gaagccgcag gcgctggact ggttgttctg
    11461 cgtggcggcg ggatatccgt ttaatgttag ctgcgacaat ctggaagggg atttcgaacc
    11521 ggaccgcgtg gtgtttcaac ggcgggtaca tgcgcaggta atggattatc tggcaaacgg
    11581 cattcccgaa cgtccggcac gcttcattaa agcactacaa aattattatc acacgccgga
    11641 acttacggcg gaacagttcc cgtggccgga agcgctcaac tgaagccagc gccacgtttt
    11701 acaatgagga aagaagatga tcgcggagtt tgaatcacgc attctggcat taatcgacgg
    11761 tatggttgac catgccagtg atgatgagtt gtttgccagt gggtatttgc gtggccacct
    11821 gacattagcc atcgcagaac tggaaagtgg tgatgaccac tccgcccagg cggtacatac
    11881 aaccgttagc cagagtctgg aaaaagcgat tggtgccggt gagttgtcgc cgcgtgacca
    11941 ggcgctggtg accgatatgt gggaaaacct gtttcagaag gcgtcacagc agtaagtaat
    12001 gtgccggata aggcgttcac gccatatccg gcaagcaatg ccagagctga ttaccccgcc
    12061 ttaaccgctt tacctttcaa caacttccgc acccataacc gattcgggtt taacgccgcc
    12121 agcgtactgg catccatcgg aattggttcg ttgctcatct gcgccgccag aatctcggca
    12181 cacaacgggg cagagcacaa accgcgagaa cctaaagcag ctaacataaa aagatcgtca
    12241 taaattggcg cgcttactgc ctcatctttc tgctccgcca gcgaagcata ttccacgagc
    12301 gttacgtcat aatcgggcac gttgcctacc attggcagat gatcgcgggt ggcacaacgc
    12361 acaccgcagc gcgcctcttt ctcactgaca tcaacctctt ttgcccactg cgcctgcggg
    12421 aaacaatcaa tcaaccgctg gcgattctgc tgctgatcgt cctcactgta cgccgtatct
    12481 tcgctgccgc gatgataact ggcaccaata caatgatgtt gattcgccgg attttgtggc
    12541 gtgagataac cgtcatagca cagcacctgc ttcagctccg ctagttccgg cgttgtcgga
    12601 atatggctaa cctgcccggc aaccgaatac actggcagag acgatgtttg gctgaaacgg
    12661 ctgatttgat gcccgttcgc cagtaccacc acgctatgcg ttgcttgctg atctcctgca
    12721 aaattcagca accaacagtc atccttacgg gataaatcct ttaactggtg ttgataacga
    12781 atctgtaaac cttgctgttg cgccagtttc agcacattac aggtcagttc tgctggacac
    12841 agccatccac cttgcggata tgtaataccg ccacaatttg ttactacacc tgtaacgcct
    12901 tcaacctcat tcgccccgac cgcaacagcc agttctgtgg gtaaatccat cgacagcatc
    12961 tgtgcgattt tatgctggct tttctcatcc cagcctaact gcgtgacgcc gcaccagtca
    13021 tgatcaaatt tgacgggcaa taaatcgtaa agccgacgag caaaagtaaa cgcattagag
    13081 aaaaagcggt tcaacgcctc atcgtgttta cttaagagcg gatacagcgc cccctggcga
    13141 ttgccggaag cacccagcgc gggggcttca tccgcgcaat aaagcgttac ttgccagccg
    13201 cgccgtaata acgccagcga caacaacgcg ctggcaatac caccgccgat aatcgctact
    13261 tcccgtttgc tgctgcccgt acgattaaac cacggcgtgg agcaggggag gggtaatgtc
    13321 tgttccatca ccccacaaag catttcccgt ttgcgcccaa agcccttacg tttttgcatc
    13381 gtgaatccgg cgtcctgcaa accgcggcgg acaaaaccgg cagacgtaaa tgtcgccagc
    13441 gtgctgcccg gacgcgccag tcgtgccatg gcgttaaaca gattttgcgt ccacatatcc
    13501 gggtttttcg ctggcgcaaa gccgtccaga aaccaggcgt ccactttttg attcagcgag
    13561 tcgtccagtt ggctggtcag ttcgtttata tcgccaaacc ataaatccag tgtcacgtgg
    13621 ccttcatcga gcaataaacg atggcaaccg ggcaagggca ttggccactg cgcctgaagt
    13681 tgttctgccc acggagccag ttccggccag tgtagatgcg ctaaggctaa atccgcacgg
    13741 gtgaggggaa atttctcaaa actaatgaaa tgtaagcgtt gtaattgcgc ttgcggatgt
    13801 gcttcgcgaa actgatcaaa tgcctgccat agtgtcagga agtttaatcc ggtgccgaag
    13861 ccgctctctg ctaccacaaa cagaggatgt ggatgctcag gaaagcgtgc ctctaattgg
    13921 ttacctccga gaaaaacata acgcgtctct tccagtccgt tgtcattgga aaagtaaacg
    13981 tcgtcaaaat ctcgggaaac aggtgtaccc tcagcattaa attcgaggtt ggcaggttgt
    14041 atggcgtagt gtttcacgta agttactcgt ctgacgggca gtctgacgat cttaacgatg
    14101 tgtgtcaggc tgcgcaaatt tctctattaa ttggctgatc ggacttgttc ggcgtacaag
    14161 tgtacgctat tgtgcattcg aaacttactc tatgtgcgac ttacagaggt attgaatgaa
    14221 acgtgcagtg attactggcc tgggcattgt ttccagcatc ggtaataacc agcaggaagt
    14281 cctggcatct ctgcgtgaag gacgttcagg gatcactttc tctcaggagc tgaaggattc
    14341 cggcatgcgt agccacgtct ggggcaacgt aaaactggat accactggcc tcattgaccg
    14401 caaagttgtg cgctttatga gcgacgcatc catttatgca ttcctttcta tggagcaggc
    14461 aattgcagat gctggcctct ctccggaagc ttaccagaat aacccgcgcg ttggcctgat
    14521 tgcaggttcc ggcggcggtt ccccgcgttt tcaggtgttc ggcgctgacg caatgcgcgg
    14581 cccgcgcggc ctgaaagcgg ttggcccgta tgtggtcacc aaagcgatgg catccggcgt
    14641 ttctgcctgt ctcgccacgc cgtttaaaat tcacggtgta aactactcca tcagctccgc
    14701 gtgtgcgacc tccgcacact gtatcggtaa cgcagtagag cagatccaac tgggcaaaca
    14761 ggacatcgtg tttgctggtg gcggcgaaga gctgtgctgg gaaatggctt gtgaattcga
    14821 cgcgatgggt gcgctgtcta ccaaatacaa cgacaccccg gaaaaagcct cccgtactta
    14881 tgacgctcac cgtgacggtt tcgttatcgc tggcggcggc ggtatggtgg tggtcgaaga
    14941 actggaacac gcgttggcac gtggtgctca tatctacgct gaaatcgttg gctacggcgc
    15001 aacgtctgat ggcgcagaca tggttgctcc gtctggcgaa ggcgcagtgc gttgcatgaa
    15061 gatggcgatg cacggtgttg ataccccaat cgactacctg aactcccacg gtacttcgac
    15121 tccggttggc gacgtgaaag agctggctgc tatccgtgaa gtgttcggcg ataagagccc
    15181 ggcgatttct gcaaccaaag caatgaccgg tcactctctg ggcgctgctg gcgtacagga
    15241 agctatctac tctctgctga tgctggaaca cggctttatc gccccgagca tcaacattga
    15301 agagctggac gagcaggctg cgggtctgaa catcgtgacc gaaacgaccg atcgcgaact
    15361 gaccaccgtt atgtctaaca gcttcggctt cggcggcacc aacgccacgc tggtaatgcg
    15421 caagctgaaa gattaattcg gagtaggtcg gataggacgc gtcagcgtcg catccgacga
    15481 tatgcggcaa tgctgcctcc ggtactaaag aataagggaa ccctatggtt ccctttttca
    15541 catcattgac aatcgccgcc tgtaccaggc aaacttcccg ctttgtcgat ttccttctga
    15601 aaagacgtac gcgttaattc ctgccaacgc accgtaaccc tgaaaccaga gagatgagac
    15661 ggggatactc ctcgccttgc gctgcattct ggagtaatgc atgactgctg taagccaaac
    15721 cgaaacacga tcttctgcca atttttcgct cttccgcatc gcttttgcgg tttttctcac
    15781 ctacatgacc gtagggttgc cgttgccggt tatcccgctg tttgttcatc atgaactggg
    15841 ctatggcaac actatggtcg gcattgccgt cgggattcag tttctggcta cggtgctgac
    15901 gcgtggttac gccgggcgac tggccgatca atatggcgca aaacgttcgg cgcttcaggg
    15961 gatgttagct tgtggtctgg cggggggcgc gttgctgctg gcggcgattt tgcccgtccc
    16021 cgcaccgttc aaatttgccc tgttggtcgt cgggcgtttg attcttggct ttggtgaaag
    16081 ccagttactg acgggcgctc tgacctgggg attaggcatc gttgggccaa aacattccgg
    16141 caaagtgatg tcatggaacg gaatggcgat ttacggtgcc ctcgctgttg gtgcgccgct
    16201 tggcctattg attcatagcc attacggttt tgccgcactg gcgctcacca caatggcatt
    16261 acccttactg gcgtgggcct gtaacggcac agtgcgcaaa gtaccggccc tggcggggga
    16321 acgtccatcg ctgtggagcg ttgtcgggct tatctggaaa ccagggttag gtctggcact
    16381 acaaggcgtt ggttttgcgg ttatcgggac tttcgtttcg ctctactttg ccagcaaagg
    16441 atgggcgatg gcgggcttta cgcttaccgc gtttggcggc gcatttgtcg tgatgcgcgt
    16501 catgtttggc tggatgccgg accgttttgg cggcgtgaaa gtggcgatcg tctctctgct
    16561 tgtagaaacg gtgggcttgt tgctgctctg gcaagcccca ggtgcatggg tcgcattagc
    16621 gggcgcggcg ttaaccggag ccggatgttc gcttatcttt cctgcgctgg gcgtggaggt
    16681 ggttaaacgc gtaccgtcac acgttcgcgg caccgcactg ggcggttacg ccgcgtttca
    16741 ggatatcgcc ctcggcgtct ccgggccgct tgcgggaatg ctggcgacca cgtttggtta
    16801 ctcttcggta tttcttgccg gggcgatctc tgcggtgctg ggaattattg tcacgatact
    16861 gtcatttcgt cggggttaac gaaccagcca gaccagcatc aggataatcg cgactaacag
    16921 tatccacagc gcgggacgcg ttgccatatt ttgcaacgtg tccatcatcg gtggaaaagg
    16981 attaagcagc ggttgcatat cacgcggatc aatcccttta acccgccgtt gataaagctg
    17041 attaagaata tcctcggcct gggcagaaga aagcgatgat tgcgcagaaa ggccatattt
    17101 ttgctggata tatgccgata acgccgccag ttcactggca tctaaaggtt gtttcagcgc
    17161 catctgtagt gattcaagcg tcggcgtatt ttgctggctt agcgtctgac gcgcctgcag
    17221 ccaggtcacc agatggttaa acagtttcgc tggaattaac tcgccatctt tcaccccgga
    17281 aagttccagc atcgattgcc agatctgttt gctgggttct cccgttgccg ccgcaagttt
    17341 ggtcaccagc tgtttgagcg cattgtgctc cgccggtaat aacggacggt cggtcgcctc
    17401 gcgctgctgc ggttgcggaa taaccatctt cccttcctgc aacagggtta gaatggtttt
    17461 taattgctcc ggtgagagct gattcagcgg cgtctgacca aagttatgac ggatataatc
    17521 cgtgaccgcc tgacgattat tccccagacg taaatactcc cctaattgcg ctaaaagctg
    17581 gcgggcagaa tggctttttt gcgcggccag cagacgttgc gccagattgt gctcagcggc
    17641 agggaagtga cgcgaaagca gcggtgaatc tcccgacaga ccaatatcgt gcctgatacc
    17701 cgcccacagt tctgctcttt gctgttgcgt cagtgaggtc actttcgtca ttaagctttc
    17761 cagcgaagta cgttgctgac tggataaagg ctgattgccc gcgccagacg gcaggttatc
    17821 tccctgacct ggtggttgcc caggaggagg gccggaaata ggttgtatca ttacgtatcc
    17881 ttatacctga aatcttcgca agtatgcctg gccgcgagat tatggcacac ttgtccggtt
    17941 aactctcgtc tcatacaggt aacacaaacg tgaaaatcct tgttgatgaa aaatgaaaat
    18001 tatttaacat tttgatataa aaggatttat atcaattgtt gtccacgcaa tgaccacatt
    18061 ttcgagatat agaaaaagcc cgcagttttt acgctgcgg
//
LOCUS       DAZGTJ010000095        18075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000095 DAZGTJ010000000
VERSION     DAZGTJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.095"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHK8E_004048"
     CDS             complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHK8E_004048"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP0346250.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHK8E_004049"
     CDS             complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHK8E_004049"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP0346251.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHK8E_004050"
     CDS             complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHK8E_004050"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4048..4050),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0346252.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4867..5748
                     /gene="yddG"
                     /locus_tag="ACHK8E_004051"
     CDS             4867..5748
                     /gene="yddG"
                     /locus_tag="ACHK8E_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP0346253.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6021..7409
                     /gene="narU"
                     /locus_tag="ACHK8E_004052"
     CDS             6021..7409
                     /gene="narU"
                     /locus_tag="ACHK8E_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP0346254.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7504..11244
                     /gene="narZ"
                     /locus_tag="ACHK8E_004053"
     CDS             7504..11244
                     /gene="narZ"
                     /locus_tag="ACHK8E_004053"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP0346255.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11241..12785
                     /gene="narH"
                     /locus_tag="ACHK8E_004054"
     CDS             11241..12785
                     /gene="narH"
                     /locus_tag="ACHK8E_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0346256.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12785..13480
                     /gene="narW"
                     /locus_tag="ACHK8E_004055"
     CDS             12785..13480
                     /gene="narW"
                     /locus_tag="ACHK8E_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0346257.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13477..14157
                     /gene="narI"
                     /locus_tag="ACHK8E_004056"
     CDS             13477..14157
                     /gene="narI"
                     /locus_tag="ACHK8E_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0346258.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14237..15130
                     /gene="yddE"
                     /locus_tag="ACHK8E_004057"
     CDS             14237..15130
                     /gene="yddE"
                     /locus_tag="ACHK8E_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP0346259.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHK8E_004058"
     CDS             complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHK8E_004058"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP0346260.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16246..16815
                     /gene="yddH"
                     /locus_tag="ACHK8E_004059"
     CDS             16246..16815
                     /gene="yddH"
                     /locus_tag="ACHK8E_004059"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP0346261.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16969..17349)
                     /locus_tag="ACHK8E_004060"
     CDS             complement(16969..17349)
                     /locus_tag="ACHK8E_004060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0346262.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
     gene            complement(17931..>18075)
                     /locus_tag="ACHK8E_004061"
     CDS             complement(17931..>18075)
                     /locus_tag="ACHK8E_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0346263.1"
                     /translation="LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDKNYLASVLAGS
                     GLS"
BASE COUNT         4304 a         4730 c         4560 g         4481 t
ORIGIN      
        1 ggcgcttctt aaagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
       61 ctctcttttt tcgcttccgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc
      121 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat
      181 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtgaatc
      241 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
      301 atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttc
      361 tgcccggcgt tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg
      421 acaatgttca acagccacgg aatatccttc ttatccggga tattgtgatg tacaaaacgc
      481 acaaacataa acatcagcgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
      541 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
      601 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
      661 ataaacttgg tgcgcacaat cattttcgac ttactcatga tgctcctcct cgtcatcgtc
      721 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcgat
      781 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aagttcaccg atgtgtcgat
      841 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcaacacgta
      901 catgacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
      961 cgctttcagt ttcgctacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
     1021 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
     1081 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
     1141 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcaac
     1201 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg
     1261 atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc cgttctgttc
     1321 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
     1381 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
     1441 acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta
     1501 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
     1561 cgtttccata gccatctgtt cgccccctta cgccttctcg atgtttacta agaacgcttt
     1621 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
     1681 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
     1741 ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg cgcggataaa
     1801 gccacgcttg ctggagacag tgacacgatc gccattggca atgcctttcg ccgccgccag
     1861 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
     1921 ggtccaggtg tggaagtgct ccgtcagacg ataggtcgta cccacatacg ggaactgctc
     1981 ttttttaccc atccgcagtg cgtcttgttc atacagacga acaaccgggt tagacaccac
     2041 gttcggatgc aacgggttag tgcccagcgg cgtttcaatt ggctcatagt gttccgggaa
     2101 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
     2161 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt
     2221 ccacttgcta ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
     2281 atccgccgaa gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc
     2341 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
     2401 ctcggtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
     2461 atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata
     2521 gagatcttcc agcgcatagc cgttgttctc tttcgccact tcgtcagact gcggttcgtg
     2581 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccctcggc
     2641 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgtgc
     2701 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
     2761 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
     2821 tgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac
     2881 cagcggatcg ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt
     2941 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttaccttc
     3001 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
     3061 gtagccccag ttgttctctt tctgcgcggc atcgccgtag aaagatttca tcaggctaac
     3121 gaaaaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
     3181 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
     3241 tggcaggctg gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg
     3301 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
     3361 catcgtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa
     3421 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
     3481 ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc
     3541 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
     3601 gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta
     3661 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcattggt
     3721 gtaatgctta acgtattcgg cattgatttt gttgttttcg atcaggtagc gcaaaacgcc
     3781 agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc
     3841 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
     3901 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccattac
     3961 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
     4021 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ctgccagcat
     4081 cccgagggag cgggcaaatt tttgagtcag catcccggtt tcgttgctgg caccggaggc
     4141 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
     4201 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
     4261 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
     4321 ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
     4381 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc
     4441 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
     4501 ctctttcgcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
     4561 gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
     4621 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
     4681 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
     4741 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
     4801 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
     4861 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
     4921 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct
     4981 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
     5041 cagatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
     5101 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt ggggatggtg
     5161 aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
     5221 aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggt
     5281 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
     5341 tatttcctgt cgtttattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa
     5401 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg
     5461 gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa
     5521 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaacgt cggtatatta
     5581 catggcaacg tcaccattat ggcggtaggt tcgtatttta cgcccgtcct ttcctcagcg
     5641 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
     5701 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgtcgg
     5761 gtctacggcc cggcagttaa tcagataaat tctgacaata ttaaaaaata aatttcaaat
     5821 accagaaagc cattttagtt acatttagta acacatatct gtctgtttaa tccacattct
     5881 gcccccgcag ttacgctttc ttatctattc ttctaatcag cacttcacct gattcatcct
     5941 taattctaat aactggctat tgcgccttac ctcctgtttt gtcttttttt cattaccaat
     6001 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
     6061 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaagcat attgcacgaa
     6121 gaaacctctg gatatcagtc agttgcctac ttcttgcctt ctgtgtctgg atgctattta
     6181 gcgcagttac tgttaatctc aataaaatcg gttttaattt cactaccgat caactctttt
     6241 tattaaccgc attaccctcc gtttctggcg cattattgcg tgtcccctac tcctttatgg
     6301 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     6361 gcgtctggct cggaattgcc gtgcagaatc cgaatactcc ttttgggata tttatcgtta
     6421 tcgctttgct atgcggtttt gcaggtgcga actttgcttc gagcatgggc aatatcagtt
     6481 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     6541 taggtgtaag tgtgatgcag ctggttgcac cgctggtcat ttttgtcccc gtatttgcct
     6601 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     6661 catggatttg ggtgccatta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     6721 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     6781 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     6841 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     6901 ttggcccatt tatcggtgcc atcgcgcgat cggttggtgg tgctatttcc gataaattcg
     6961 gcggcgtgcg ggtgacgttg atcaacttca tttttatggc gattttcagc gccctgctgt
     7021 tccttacctt accgggcaca ggctccggta atttcatcgc attttacgcc gtatttatgg
     7081 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     7141 ttcgccagat aacaatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaaa
     7201 gagaagccat caccgaaacg gcagccgctc tgggctttat ctcagccatt ggcgcagtgg
     7261 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtgg
     7321 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctactgacc tggctggttt
     7381 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgacacgtc ttatcaggct
     7441 acaaccgtgt cgcatccgac aaggcatcag atcccaattc attcctggag caggagttat
     7501 gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga aacctttgcc
     7561 gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt
     7621 tggcagttcg acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc
     7681 tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg
     7741 cgcactcgcc ctgacctgcc aaatcatgaa cctcgcggct gcccgcgtgg cgcaagttac
     7801 tcctggtatc tctacagcgc taaccgcctg aaataccctc tcattcgtaa acgactgatt
     7861 gaactgtggc gcgaagccct caagcaacac agcgatccgg tactggcgtg ggcatcgatt
     7921 atgaacgatc cgcaaaagtg cctgagctac aaacaagtac gtggacgcgg cggctttatc
     7981 cgctctaact ggcaggagct aaatcagctg attgctgccg ctaacgtctg gaccatcaaa
     8041 acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct
     8101 tacgccgccg gaacacgtta tctttcacta ttaggtggca cctgtttaag cttctacgac
     8161 tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg
     8221 gaatctgccg actggtataa ctccagttac atcatcgcct gggggtctaa cgtaccgcag
     8281 acacgtacgc cggacgccca tttctttacc gaagtacgct acaaaggcac taaaaccatc
     8341 gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa
     8401 caaggcactg atagtgccct ggcaatggca atgggccatg tcattttaaa agagtttcat
     8461 ctcgataatc ccagcgatta ttttattaac tactgccgcc gctacagcga catgccgatg
     8521 ctggtaatgc tggaacctcg cgacgacggc agctacgttc ccgggcgcat ggtccgcgca
     8581 tctgacctgg tggatggact gggcgaaagc aataatccgc agtggaaaac cgtggcagtt
     8641 aataccgcag gtgaactggt agtgccgaac ggttcgattg gttttcgctg gggagaaaaa
     8701 ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccgaattgtt gttaactctg
     8761 ctcggtcaac atgatgccgt tgcaggcgtg gccttcccct actttggcgg cattgaaaat
     8821 ccgcattttc gcagcgtaaa acacaatccg gtcctggtgc gccaattgcc cgttaaaaac
     8881 ctgacattag ccgatggcaa cacctgtccg gtcgtcagcg tttatgattt ggtactggcg
     8941 aattatggcc tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta cgctgaaatc
     9001 aaaccgtaca ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa
     9061 accatcgccc gtgaatttgc cgataccgcc cataaaacgc atgggcgctc gatgattatc
     9121 ctcggtgctg gcgtcaacca ttggtatcac atggacatga actaccgtgg gatgatcaac
     9181 atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggt
     9241 caggaaaaac tgcgcccgca aaccggctgg ttgccgctgg cctttgcgct cgactggaac
     9301 cgaccaccgc gccagatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc
     9361 tatgaaaaag tctttgctca ggagttactt tcaccgctcg ccgatgccag taagtacagc
     9421 ggtcatctga ttgatttcaa cgttcgtgcc gaacgtatgg gctggctacc ttctgcgccg
     9481 cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggccgg attatccccc
     9541 acagaattta ccgcccaggc gctgaaatcg ggcgatttac gcatggcctg cgaacaacca
     9601 gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gttctaacct gcttggctcc
     9661 tccggcaaag gccacgagta tatgcagaag tatctgctgg gtacggaaag tggcattcag
     9721 ggggaagaac ttggaccgac ggagggaatc caaccggaag aagttgagtg gcaaactgca
     9781 gcgattgaag gcaagctcga cctgctggtg acgcttgact tccgcatgtc cagcacctgc
     9841 ctgttctccg atatcgttct gcccaccgcc acctggtacg aaaaagacga tatgaatacc
     9901 tcggatatgc atccgtttat tcatccgctt tctgcggcgg tcgatccggc gtgggaatca
     9961 cgcagcgact gggaaatcta caaaggtatc gctaaagcct tttcgcaagt gtgcgtgggg
    10021 catctcggca aagaaaccga catggtatta caaccactgc tgcatgactc cccggcagag
    10081 ctttcacagc cgtgtgaagt gctcgactgg cgcaaaggtg aatgcgatct gattccaggc
    10141 aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctaccta tgaacgcttt
    10201 acctcactcg ggccattgat ggacaaactt ggcaacggcg gtaaagggat ttcgtggaat
    10261 acccaggatg aaatcgattt cctcggtaaa ctcaattaca ccaagcgtga tggcccggca
    10321 caggggcgtc cgctgattga taccgcgatt gacgcatcag aagtgattct ggcgctggca
    10381 ccggaaacca acggccatgt tgcagtcaaa gcgtggcaag cgctgggcga gatcaccgga
    10441 cgcgaacata cccatctggc gcagcacaaa gaggacgaga agattcgctt tcgcgatatt
    10501 caggcgcagc cgcgtaaaat tatctccagc cccacatggt ctggtctgga aagcgatcac
    10561 gtttcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg
    10621 ggacgccagc aactctatca ggatcatccg tggatgcgtg cttttggtga aagtctggtg
    10681 gcttatcgcc cgcctatcga cacccgcagt gtcagtgaaa tgcgccagat accgccaaac
    10741 ggcttcccgg agaaagccct taacttcctg acaccgcacc agaaatgggg cattcactca
    10801 acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc
    10861 agcgaaaccg atgcccgtga actgaccatt gtcgataacg actgggtgga agtgtttaac
    10921 gccaatggcg cgctgacggc ccgcgcggtg gtcagccagc gtgtaccacc gggcatgacc
    10981 atgatgtatc acgctcagga acgcattatg aatattccag gttcggaagt aaccggcatg
    11041 cgcggcggca ttcataactc ggtcacccgc gtttgtccga aaccaacgca tatgattggc
    11101 ggttacgcgc agctggcctg gggctttaat tactacggca ccgtcggctc gaaccgtgac
    11161 gaattcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat
    11221 caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg cttaacctcg
    11281 ataaatgtat cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg
    11341 aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga
    11401 aaaactggga agatcaggaa gaatggcaag gcggctgggt gcgtgatgtg aatggcaaga
    11461 tccgcccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg
    11521 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg
    11581 caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga
    11641 tggacaaagt gatctggggg ccaaactggg aagaactgct gggcggcgaa tttgaaaaac
    11701 gcgctcgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca
    11761 ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga
    11821 cctgcccaag cggtgctatc tacaaacgtg aagaagacgg cattgtgctg attgatcagg
    11881 ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca
    11941 actggaaaag cggcaagtca gaaaaatgta tcttctgtta cccacgaatt gagtcaggcc
    12001 aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt
    12061 acgacgccga ccgcatcgag gaagcggcga gcaccgagcg cgaagttgac ctctatgaac
    12121 gtcagtgcga agtgttcctc gatccgcacg atccctcagt gatcgaagaa gccctgaaac
    12181 aaggtattcc acaaaacgtg attgaagctg cccagcgttc gccagtctac aaaatggcga
    12241 tggactggaa actggcgtta ccgctgcacc ctgaataccg caccctgccg atggtctggt
    12301 acgttcctcc gctgtcaccg attcagtctt acgctgatgc gggcggtttg ccgaaaagcg
    12361 aaggcgtgct gcctgctatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt
    12421 tgagtgctgg cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc
    12481 actatatgcg ttcacaaacc gtggaaggcg ttaccgatac tcgtgccatc gacgaagtag
    12541 gcctgaacgt cgcccaggtc gaagagatgt atcgctacct cgccattgcc aactatgaag
    12601 accgttttgt cattccaacc agtcatcggg aaatggcggg cgatgccttt gctgaacgca
    12661 acggctgcgg ttttaccttt ggcgacggtt gccacggctc ggacagcaaa ttcaacctgt
    12721 tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
    12781 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg
    12841 ggaatgtaag gaggacgcgc tggcgttgat ccgccgcgac gcaccgatgc tcaccgattt
    12901 tgcactcgac ctgctcaacg cgccgctgct ggataaacag gccgaatggt gtgaagtgtt
    12961 tgaccgcggg cgcaccacgt cgctgctgct gtttgaacat gttcatgccg agtcccgcga
    13021 tcgcggccag gcaatggtgg acctgctggc ggagtatgaa aaggtcggtc tgcaactgaa
    13081 ttgtagggaa ctgcccgatt atctaccgct gtatctggag tatttaagca tacttccgga
    13141 cgatcaggcg aaagaaggat tactcaacgt tgcgccgatc ctcgccctgc ttggcggacg
    13201 cttaaaacaa cgcgaggcac cgtggtacgc gttatttgac gccctgttgc aactggcagg
    13261 aagccctcta tcaagtgaca gtgtcacaaa acagattcgc agtgaagagc gtgacgacac
    13321 ccgccaggcg ctggatgcgg tgtgggaaga agaacaggtg aagtttattg aagataacgc
    13381 cacggcgtgt gacagctcgc cgcttaatca atatcagcga cgctttagcc aggatgtcgc
    13441 gccgcagtat gtcgacatca gtgcgggagg tgggaaatga ttcagtatct gaacgtcttt
    13501 ttttacgata tctacccgta cttgtgtggc acggttttta tccttggaag ctggctgcgc
    13561 tacgattacg ggcagtatac ctggcgtgcc gcctccagcc agatgctgga taaacgcggg
    13621 atggtgctat ggtcgaactt gttccatatc ggcattctgg ggatcttctt cggtcatctg
    13681 ttcggcatgt taacgccgca ctggatgtac gcgtggtttc tgccggtggc ggtaaaacag
    13741 cagatggcga tgatcctcgg cggaatatgc ggggtattga cgctgattgg cggtgcgggg
    13801 ctactggtgc gtcgtttaac aaatccgcgt atcagggcga cgtcttctgg tgcggatatc
    13861 ctgatcctcg ccattttgtt aattcagtgc attcttgggt taaccaccat tcctttttca
    13921 gcacaacatc cggacggcag tgaaatgatg aagctggtgg gctgggcgca aagcattgtc
    13981 actttccagg gcggtgcatc tacgcatctt gatggtgtcg cctttatatt ccgcgttcat
    14041 ctggtgcttg gaatgaccat cttcctgctc ttcccgttca cccgactggt gcatgtctgg
    14101 agcgcgccgt ttgagtactt tactcgtcgg tatcagattg tgcgttcgcg gcgttaattc
    14161 tcacgcgggc cggacagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
    14221 ccacaggaga acattaatga aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
    14281 gtttcgcggc aattctgccg gtgttgtttt ccccgccgat aatctcagtg aagcgcaaat
    14341 gcagcttatc gcccgcgagt taggtcattc ggaaactgct tttctgctgc acagcgacga
    14401 cagcgatgtg cgcatccgct actttacgcc aacggttgaa gttccgattt gtggtcacgc
    14461 gacagtagct gcgcactatg tacgtgcaaa ggtgttaggt ttaggaaatt gcacggtctg
    14521 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg atgattatcg
    14581 tatttcgctg gaacaaggta cgccgggctt tgagtcaccg ctggaaggtg aaacacgtgc
    14641 ggcgattatc aacgcgcttc atctcactga agacgatatt ctgccaggct tgccaattca
    14701 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
    14761 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
    14821 tttcttcccg ttccagatcc gcccagacaa aaacgaaacc gacggtcgta tgttctcgcc
    14881 tgccattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
    14941 gttagtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
    15001 ggggcgcgca ttagggcgtg acggcgtgat tgaagtgacg gtgacaattc gtgataacca
    15061 accggagaaa gtcaccattt ctggcgtggc ggtgattttg tttcatgccg aatgggcaat
    15121 tgaactctga aaccgttgcc tgatgcgctt cgcttatcag gtctacaacc gggctaatgt
    15181 aggccggata aggcgtttac gccacatccg gcaacctgac ataaattatt ttcccgcctc
    15241 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacagtaa agccatgttt
    15301 cgcatcatct acgcccagac caaactgttc ctgcatcacg gcatataacg acgccacatc
    15361 tggtagattt cgctgctcaa ctgcgtgccc attttcataa tgggtaaaat gaaaattggt
    15421 cagcgttagc ttgccaccgt ccggcaaatg gcgacacatc agcaaatgat ggcgaaaatg
    15481 cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt gttgctgctc
    15541 gcagagatca aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca
    15601 atcatcaccc tcttgcaaca accgatactc tccgtgtggc gtggtttgca tgatatccgg
    15661 cattaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt
    15721 ttccccttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt
    15781 tgataacact acgcgcccta acaagctgcg aacgttaaac cccagctcgc gtaacacccg
    15841 ctcaaacacg ccattctgct caaaacagta accaccacga cgggctatca ccagtttctc
    15901 ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcga ggttttcaaa
    15961 cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tggccgcagc
    16021 agctcccgac cagttaatac gggcaaaata atgatttaga atgggcgtca tcaggcgttt
    16081 cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcaccggct attttgtgcg
    16141 atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtt acttattgcg
    16201 ttacgagcta cgcttttaaa acacccttca tagcgaggcg atatcgtgag ccaattcatt
    16261 cccgtcgaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc
    16321 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    16381 gagtttgaac ctccacgcgt ggcgattgtg gtagataagt cgacatggac cagggagttg
    16441 attgagcgta acggtaaatt tggcatcgtt atcccaggcg ttgcagcgac taactggacg
    16501 tgggcagtgg gaagtgtatc tgggcgtgaa gaggataaat tttattgcta tggcattccg
    16561 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgtct ggcgtggatg
    16621 gagtgccgat tgctaccagc gacttccgcg caagagaaat acgacacgct gtttggcgaa
    16681 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    16741 gataagctca atacgttgca tcatttaggc gctggcacgt ttgttaccag cggcaagcga
    16801 gtcaccgcgg gctaaagaat catatgcagc aaccaagttg cttaagcaat gtttcaatat
    16861 gagttaaaag catattttat ctcacgattg catattttta gtgaagatta atttatcctc
    16921 accaacatca tagtaattgc gtattatagc ttcattgata aagccctgtt atgtataaaa
    16981 tgcgcgggcg gcactaaaat cgacatagct ggaggtctct actaaaagta gtcttgcctg
    17041 tttaattgtg agctcctttt ctatttcgtt aatcagcaga tgtcccactc cctggcgctg
    17101 atgggaagga ctgatccagt gcattaataa attccagaca tcatttgtca tcacttcagg
    17161 agcgcaatag gcaatccctg ccagtcctaa gtgttctgca ctaaaccata aatcggcaga
    17221 ttgacctgag aaataatgtg ttagtgtttt ttgtacatga tgaaggccat cttgattaaa
    17281 ttgtccactg gcaagcagca tatccagtaa tggttcaaca tctgaaggtt tgactggtcg
    17341 tataatcatg gaattcctta tctattgatg aatgtttgaa aattgtttgc gtggaacatg
    17401 gttaatctga gccaatattt ttgagaaaga atagatgggt atttatataa gcaaagaagc
    17461 catctcttta aatatcaatt aaagtataac attaacacca gacttcattg cgacaggaca
    17521 cgacaaagca cttttgcggt tttttcaggg tgcaattaca aaaattgaat cccttatgga
    17581 ctaatgtgag aaaattccgg ggcatgacta ttttgatata gtggacaatc aagcgtctcg
    17641 aattaacaat gacttttcag atgctaaacc atgcaattgg aagtatgtcg gatataggac
    17701 aacattagga aatcggacag aaaattcatc ataataaaat gacaacttat ctctttctct
    17761 cactttctga gaaatttcac aaaagtataa actgagccaa tatgccttta tatataaaaa
    17821 atactcatgc ttatttttat cggaaaaagc caagattttg tcaaggccat caaaaatatc
    17881 tttatcatga tctatatgta ctcttgatga tttcacaata cagggaaaga ttacgaaagc
    17941 ccgctccccg caaggactga cgccagatag tttttgtcca tggctgcttt tcgcatctta
    18001 cgtcttaacc ctgccttgaa taccttatca ttcgttaaaa tattaatagc gatgtgccgt
    18061 atccctgaaa ataat
//
LOCUS       DAZGTJ010000096        17707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000096 DAZGTJ010000000
VERSION     DAZGTJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.096"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..281)
                     /gene="yddM"
                     /locus_tag="ACHK8E_004062"
     CDS             complement(<1..281)
                     /gene="yddM"
                     /locus_tag="ACHK8E_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0346264.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
     gene            complement(281..559)
                     /locus_tag="ACHK8E_004063"
     CDS             complement(281..559)
                     /locus_tag="ACHK8E_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0346265.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            complement(745..1755)
                     /gene="adhP"
                     /locus_tag="ACHK8E_004064"
     CDS             complement(745..1755)
                     /gene="adhP"
                     /locus_tag="ACHK8E_004064"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP0346266.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            complement(1889..3586)
                     /gene="maeA"
                     /locus_tag="ACHK8E_004065"
     CDS             complement(1889..3586)
                     /gene="maeA"
                     /locus_tag="ACHK8E_004065"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0346267.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            complement(3743..3880)
                     /gene="sra"
                     /locus_tag="ACHK8E_004066"
     CDS             complement(3743..3880)
                     /gene="sra"
                     /locus_tag="ACHK8E_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP0346268.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(3982..4197)
                     /gene="bdm"
                     /locus_tag="ACHK8E_004067"
     CDS             complement(3982..4197)
                     /gene="bdm"
                     /locus_tag="ACHK8E_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP0346269.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            4542..4973
                     /gene="osmC"
                     /locus_tag="ACHK8E_004068"
     CDS             4542..4973
                     /gene="osmC"
                     /locus_tag="ACHK8E_004068"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP0346270.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(5029..5955)
                     /gene="ddpF"
                     /locus_tag="ACHK8E_004069"
     CDS             complement(5029..5955)
                     /gene="ddpF"
                     /locus_tag="ACHK8E_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP0346271.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(5948..6934)
                     /gene="ddpD"
                     /locus_tag="ACHK8E_004070"
     CDS             complement(5948..6934)
                     /gene="ddpD"
                     /locus_tag="ACHK8E_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0346272.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(6931..7827)
                     /gene="ddpC"
                     /locus_tag="ACHK8E_004071"
     CDS             complement(6931..7827)
                     /gene="ddpC"
                     /locus_tag="ACHK8E_004071"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP0346273.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(7824..8846)
                     /gene="ddpB"
                     /locus_tag="ACHK8E_004072"
     CDS             complement(7824..8846)
                     /gene="ddpB"
                     /locus_tag="ACHK8E_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0346274.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(8848..10398)
                     /gene="ddpA"
                     /locus_tag="ACHK8E_004073"
     CDS             complement(8848..10398)
                     /gene="ddpA"
                     /locus_tag="ACHK8E_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0346275.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(10412..10993)
                     /gene="ddpX"
                     /locus_tag="ACHK8E_004074"
     CDS             complement(10412..10993)
                     /gene="ddpX"
                     /locus_tag="ACHK8E_004074"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP0346276.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(11251..13650)
                     /gene="dosP"
                     /locus_tag="ACHK8E_004075"
                     /gene_synonym="pdeO"
     CDS             complement(11251..13650)
                     /gene="dosP"
                     /locus_tag="ACHK8E_004075"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP0346277.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(13675..13839)
                     /locus_tag="ACHK8E_004076"
                     /pseudo
     CDS             complement(13675..13839)
                     /locus_tag="ACHK8E_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(14216..15535)
                     /gene="digH"
                     /locus_tag="ACHK8E_004077"
     CDS             complement(14216..15535)
                     /gene="digH"
                     /locus_tag="ACHK8E_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP0346278.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(15666..17201)
                     /gene="gadC"
                     /locus_tag="ACHK8E_004078"
     CDS             complement(15666..17201)
                     /gene="gadC"
                     /locus_tag="ACHK8E_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP0346279.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(17357..>17707)
                     /locus_tag="ACHK8E_004079"
     CDS             complement(17357..>17707)
                     /locus_tag="ACHK8E_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP0346280.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT         4360 a         4552 c         4427 g         4368 t
ORIGIN      
        1 tgcgtaacca gacggcgcag tcgcgacaca tccaccgttt tttcagcctc tgccaggctc
       61 caggcgtttt gcagattcag ccacatttgc ggcgaactgc cgatcaccac ggaaagttta
      121 atcgccattt ccggagtgag cgccgctttt ccggtcagca atcggcttgc cgttgagggc
      181 gcaatttcca ttgctctggc aaactcgcgc aggctgacat taagttcgtc cagtgattcc
      241 tgaataatgt cccccgggcg gggatgattg gccattttca tcagtgatag tcctcgtaat
      301 ccagtatgta tgcatcgcca ttgacgaatt caaatgtgat tcgccagttg cccgagacag
      361 atatcgccca aattccatcg cgatcgccgc ttaacgggtg tagtcggtaa ccgggcatat
      421 cgatatcagc aagacagctt gctgcatcaa tcacagcgag tcggtggcgt aatcgtttga
      481 cttgcgtcgg tataacgccg gaggttttgc cgagaagaaa caagtcacgc aatcccttgt
      541 gtctgaaatt cattatcatc tcttcatcct tgttccttca aaaggaacac tatcactatt
      601 gttccgtgca gcgcaacaaa tttcgctacg tttttgcgaa ttgtgagggg aaatatgggg
      661 gaggaaaaga agaagagtga aattgcggaa agcgtctcgc aaaacaggct gcgacccgaa
      721 catggcagtc gcagcaaagg cctcttagcg gcggaaatca atcaccatac ggccacggat
      781 tttgccttct tccatctcgg taaagatggt gttgatgtcc gctaacggac gcagggcgac
      841 tttcggcacc actttgcctt cggcggcaaa ctggaaggct tcggttagat cctggcgcgt
      901 gccgaccagc gaaccgacca cttcaatacc atccagcaca aggcgtggga tgtccaggct
      961 catagactcc ggcggcagac cgacagccac aacacgaccg cctgctcgta cagcatcaac
     1021 cgccgagtta aacgcagctt tagctaccgc tgtcaccacc gcggcgtgag cgccaccggt
     1081 tttttcttgc acaattttgg cggcgtcttc agtgcgtgag ttaatcgcta aatcagcgcc
     1141 catttcggtt gccagtttta actgctcatc attgacatca atggcgatca ctttggcgtt
     1201 aaagacattc ttcgcgtatt gcagggcgag gttacccaga ccgccaagac cgtagatagc
     1261 aatccactga ccgggacgaa tttttgacag tttcaccgct ttataggtgg tgacccccgc
     1321 acaggtaatg ctgctggccg ccgccgagtc caggccatct ggcactttta ccgcgtaatc
     1381 ggcgaccacg atgcactctt ccgccatccc gccatcaacg ctgtatccgg catttttaac
     1441 tgaacggcag agcgtttcgt taccactgtt acagtattcg caatgaccgc atccttcgta
     1501 aaaccacgcc acgctggcac gatcgcctgg ttttaatgag gtgacacctg ggccaacttc
     1561 tgccaccaca ccgatgcctt catggcccag aattacgccg gttttgtcac caaaatcgcc
     1621 attcttaaca tgaagatcgg tatgacatac accacaacac tccattttca gcagggcttc
     1681 gccatgtttc agtgagcgca gtgttttatc cgtaacgtca acatgatgat ccttcgtaac
     1741 aactgcagcc ttcatagttc ctccttttcg gatgatgttc tgcatagcag gtgaggcaaa
     1801 tgagatttat tcgccactac ccagtatgga tgagatctga aaaagggaga ggaaaattgc
     1861 ccggcagctt tcaccaccgg gcgcaggctt agatggaggt acggcggtag tcgcggtatt
     1921 cggcgtgcca gaaattatca tcaatggcct gttgcagagc ttcggcagac gttttcaccg
     1981 ccacgccttg ctgctgcgcc attttgccaa ccgcaaaggc aattgcgcgg gagactttct
     2041 gaatatcttt cagttccggt agtaccagac cttcgccgtt caggaccagc ggcgaatact
     2101 gagcaagcgt ttcacttgcc gacatcagca tctcatcggt gatacgtgac gcgccggaag
     2161 caataacgcc cagcccgatg cccgggaaaa taaaggcgtt gttacactgg gcgatagggt
     2221 agattttatc tttccatacc actgggttaa acggactgcc agtggcgacc agcgcgttac
     2281 cttcagtcca ggcgataatg tcctgcggtg tggcttccac gcgtgaagtc gggttagaca
     2341 gcggcatcac gatcggacgc ggacagtgtt tatgcatctc acggataatc tcttccgtaa
     2401 acagcccggt ctgtcctgag acgccaatca gaatatctgg ttttacatta cgcaccacat
     2461 ccagcagtga cagcacatcg ctgtcggtat cccagtcact gaggttttcg cgcttctgca
     2521 ccagtttggt ctggaaaggc agcaggttcg gcatcttgtc ggtcagcagg ccaaagcgat
     2581 caaccataaa gactttctgc cgcgccgctt cctcgcttaa tccttcacgc tgagtctggg
     2641 cgatgatcat ttcggcaatg ccgcaccccg cagagcctgc gccgaggaag acaatctttt
     2701 tctcgctcag ttgaccacct gccgcgcggc tggctgcgat cagtgtgccg accgttaccg
     2761 ccgcggtgcc ctgaatgtca tcgttaaaag aacaaatttc attgcgatag cggttaagta
     2821 acggcatcgc atttttttgt gcgaagtctt caaactgcaa cagtacgtcc ggccagcgtt
     2881 gtttcacagc ctggataaat tcatcaacga attcatagta ttcgtcgtca gtgatacgcg
     2941 gattacgcca gcccatatac agcggatcgt taagcagctg ttggttgttc gtcccgacat
     3001 ccagcaccac cggaagggta tacgccgggc tgatgccgcc acaggcggta tagagcgaca
     3061 gtttaccgat cggaatgccc atcccgccga tgccctggtc accaagcccc agaatacgtt
     3121 caccgtcagt caccacaatc actttaatat tatggttcgg cacgttttgc agaatatcgt
     3181 ccatattgtg gcggttctgg taagagataa acacgccgcg tgaacggcgg tagatctcag
     3241 aaaaacgctc acaggctgcg ccgacggttg gggtataaat aacaggcatc atctcatcaa
     3301 gatgattgtt taccagacgg tagaagaggg tttcgttagt gtcctggatg ttacgcaggt
     3361 agatgtgttt gtcgatttcg gttttgaatc cctgatactg gatccatgct cgttccgctt
     3421 gttcttcgat ggtttcgacc acttccggca gtaaccccag caggttgaag ttacggcgtt
     3481 cttccatgct gaaggcgctg cctttattca acaacggaaa ttccagcagt acagggccag
     3541 cgtaaggaat ataaagcgaa cgctgtcttt ttgtttttgg ttccatgtca ctcactcttt
     3601 tttgaatatc catccctggc acgcttttat cgtctttgct ttaccgccag ggcgtcggcc
     3661 ccaagtataa agcagataaa aacaaaaaca ccattgcgca ggcaatggtg tttaatcgtc
     3721 attgaggact gatggttatg aattactttt cagcggggcg ttttctgcct gttgggttat
     3781 ttaccacgct ggatttgtca ccttcggtaa ctattttgcg ctggttagaa attttatggt
     3841 ccagtccaag aatatgacgt gcctgacggt tcgatttcat tttaactcct caatcctgtt
     3901 actagtttta aggacaacat cgccgtagcg aagaaacacg tgctaaaccc ctaaattagg
     3961 ttgccgatca agcatagcac cttaaagcgt agggtgctga ccactgacca cataattgat
     4021 cgtttgctgg tagatatcac tgaggatgtc gttatcagat gcttccaccc atttggtcag
     4081 ctccatgaga atgtcatctt cagtgacaac accgtgctct gcccgaaggc cttgctcaat
     4141 ggcattaacc agagcgggtt ctgctgttga attttctgcc tgataataag taaacatagt
     4201 gattctccgt gtctgtgtat ttatggtgtc tgctacggat cgcagattta taaagcacat
     4261 tcagcatggc aaatatttgc cgcttcgttg ttaagattag tcctggttga tgatttttat
     4321 attttaactc catgatattg ataggtatta ttattgtgat gagttgattg atattgatgc
     4381 aatatctctt gagttatata tttccggtta tctattgtgg aaatttaatt taagtgcagg
     4441 aattatattt tcgccggatt ttattcggat tatcctgctt atcctcgtgc tgtttctcac
     4501 gtagtctata atttcctttt taagcccaca ggagagcaac aatgacaatc cataagaaag
     4561 gtcaggcaca ctgggaaggc gatatcaaac gcgggaaggg aacagtatct actgagagcg
     4621 gcgtgctgaa ccaacagccg tatggattta acacgcgttt tgaaggcgaa aaaggaacca
     4681 accctgaaga actgattggc gcagcgcatg ccgcatgttt ctcaatggcg ctttcattaa
     4741 tgctggggga agcgggattc acgccaacat cgattgatac caccgcagat gtgtcgctgg
     4801 ataaagtgga tgccggtttt gcgattacta aaatcgcact gaaaagcgaa gttgcggtgc
     4861 cgggtattga tgcctccacc tttgacggca ttatccagaa agcaaaagca ggatgcccgg
     4921 tttctcaggt actgaaagcg gaaattacgc tggattacca gttgaaatcg taaagcgttg
     4981 ccggatgacg cgtcaggcgt gtgattgcct gatgcgttgt tagcgtctct acagcgcccg
     5041 ccagcaccgg atctcacgtt cgtcctccct tattgttaat gattgccgga cttcacatcc
     5101 gtgggttgtc agagggcagc gttcgcggaa aaaacagcct tgtggcaacg tgcggtttcc
     5161 tggtagatcc gttttacgta atgcccattc ttcctccagc ggtttatcaa tggcggggag
     5221 ggaatccagc aataatcggg tgtatggatg tgccggagct gtcagcacct gttgcgcgtc
     5281 tcctagttcc acaatctgtc cgagatacat caccgccacc cgatcgctca tatgacgtat
     5341 caccgagaca ttgtgtgaaa tcagcacata agtcagctcg cgattttcct gtagcgttac
     5401 cagtaaattg aggatctgcg cctgcacgga gatatccagc gccgaggttg gttcatcaag
     5461 cacaatcacg tcaggctgcg aagagagtgc tctggcaatg gcgatacgtt gccgctgccc
     5521 gccggaaaac gcatgaggca agcggtcgag atactccgga cgaataccca cctgcacagc
     5581 cagctcctct gccaacgctc gccgctgttg ttcactacta cgcttagcaa tccagagcgg
     5641 ttccgtgatg atgcgccaca ccggtaagcg cggattaagc gaagagagcg ggtcctgaaa
     5701 caccatctgc ataatgcgtt gtgatcctga acggatgtac tgcccgtggc tcggttgcag
     5761 catacccatt aaaagctgtg cgagggtgct tttgccgcag cctgactcac cgacgatccc
     5821 taaggtttca ccacgacgga tctgtaaatc aataccattg atggcatgaa catgttccgt
     5881 agttttacca agccagtttt tacgggccgg gaaattgata tggacgtcgc gtaacgttaa
     5941 taatgtatca gacactaatg acctcctgct gcggatacca acaggcgcag cggtggttgt
     6001 tgtcaccata cgctgttagc accgggacgt tttcacactg tgcacctgcc gcatagcaac
     6061 gatcgcgaaa agcgcagccg tcaggcaaat gggtgaggtt tggtaccgtc cccggaatag
     6121 cgggtaatgg ctggcgcggt attccatgtt ccggtgcgca ttgcagcaaa ccaatggtat
     6181 acggatgccg gggatgatgg ataacgtctg ccgtcacgcc gctctcaatc acgcttccgg
     6241 catacatcac gtaaacgcta tcgcacagtt gcgacaccac ggccatatca tggctgatga
     6301 acagtaccgc agttccactg gcgcgggctt tatgtttaag cagacgcagt acctgcaact
     6361 gtaccgtgac gtccagcgcc gtagtcggtt cgtcggcaat aatcaattgc ggctcgcagg
     6421 agaatgccag cgcaatcatt acccgctggc gcataccacc tgaaagctca aacggatagc
     6481 gcgacataac ttccacggca tccgggattt gcatctcttc cagcagggca atcgctttag
     6541 ctctggcttc ccgacgactt attggttgat gatggcggat cacgtccatc atctgaagac
     6601 ctattcgacg tgtcggatta agggcggtca tcggttcctg aaagatcatc gccactcgtg
     6661 cgccgcgcca ctgacgaagc tgcttttccc gagcgttaag aacatcttct cccagcagtg
     6721 aaatatgtcc ccgatgtacg caataactgc ccgtcggtag cagacgcata atcaacattg
     6781 cagtgactga tttacccgag ccggattctc ccaccagacc gacaatttca ccgcggttga
     6841 ttttcaatga cacattgttg agcgcgtgaa cgtcgccgtt aaaaccgggg aaactcaaat
     6901 gcagttgttg aatgtccaga acgggttggg tcatgactgc tttcctcctg ctttcgggtc
     6961 aagcagatcg cgaataccat cgccaaagag attaaacccg acggcggtaa gcaaaatcgc
     7021 tgcccccgga aatgcgcaat accaccattg atcgagcaca tagttgcgac cattcgccac
     7081 catcgccccc cattcagcac tcggttgttg agcaccaagg ccaataaatc ccaacgtggc
     7141 ggccattaaa atcgcgctac cgatatccag cgatgcctgc acaatcagcg gcggcaggga
     7201 gttacgtaaa atatgccagt tgatcagatg ccagcgtgac gcaccaaagg ttttcgccgc
     7261 ctgaacatag gtatactggc gaacaactaa tgtttgcccc cgcgccaggc gcacataaaa
     7321 gggaattcgc acaatagcaa tcgccagcat ggcgttaaac aaactcggcc cgagagcggc
     7381 tgccagtgcc attgtcagca ccagcgaagg aatcgacagc ataatgtcca tgatgcgcat
     7441 gataatcgcg tctgcgcgtc cgccaagcac accggataga cacccgagta gcgaccccat
     7501 catacctgca atggcgacca ccactaatcc ggcgagaatt gattgctgac tgccgaccag
     7561 tacgcggctg aacagatcgc gtcccacttc atcggtgcca aaccagtgcg ctgcagaagg
     7621 cggcaaaagg cgggcggtta aatcaatggc gttgggatca tgcgtcgcca gccacggtga
     7681 aaaaatcatc atcaatagca ttaatacaat gatgactgcg ccggtcacgg taagcgggct
     7741 gcctttcagc atccatacca gttttgcacc gttaaatcgc gtttgttttg gtgggcgcac
     7801 ggctgacgtt tcctcgctta gcatcattca ccacctccac gtccgatacg cggatcaatc
     7861 cacaaataga gcaaatccac caccaggttc accagcacat aagcaaacga aaccacgacg
     7921 gcaaagccca tcactgccgg gaagtcgagc gcctgtattg atgttactac ccatgcaccc
     7981 attccgggcc aggcaaaaac ggtttcggtg agcactgcgc catacaacaa atcgcccagc
     8041 gccagtccca atacggtaat cgaggggatc aacgcattgg gtagcgcata acataaaacg
     8101 atataccagc cgggcaagcc gctggcccgg gcggtacgaa tgtaatcttc actcaattgt
     8161 tccagcatag ctgagcggat ctggcgagcg acaattccca ggtgaacgaa cgccagcgtt
     8221 aatgccggta agatgagatg ttgcagcgca ttgaagaaga cttcaccgtt gccttcaagc
     8281 agcgcatcga gcagataaaa gccggtaacg tgcgttggtg gatccagcca gtcatcaagc
     8341 cttccgccgc cgggaagaat ttgcagatga ccataaaaca gcacaatgac gcccagccca
     8401 agccagaacg caggtgtgga gattccggta atggccatta ttcgcaccag atgatccagc
     8461 cagcgatttc gccagactgc agagagtatg cccagcggga tgccaatcag aagtgccagc
     8521 agcagggcac aaaaagccag ttccagcgtc gccgggaaaa atatgcgcaa ctcttccagc
     8581 accggacgcc cggtacgaat ggatgttccc aggtcaccct gaaacaggtc gctgacgtag
     8641 cggtaaaact gtacgtacag tggctggtcc agtcccagtt gctggcgaat attatccacg
     8701 atagcatcgc tggcgcggtc acccgccagt aaccgcgccg gatcgccggg gatcaggtgc
     8761 gagataataa acgttatcac acagacgccc gccaccacga gcaccagccc ccagcagcgt
     8821 tggcgtaaaa tactccagaa ggtcacgtta tttactcatg gtattgatat tgaagacctg
     8881 ttccagcatg ggattgaaca caaagccttt cacctctttg ttcatcgcca gttggtagtt
     8941 tttctggaac aggtacacat aagcagcgtc atcaatgacg attttctgtg cctgctggta
     9001 gtcccgggta cgctgcgtct ggtcggtggt cgctagcgca ttgcgcagta gcttatcgac
     9061 ctcactgttt tcatagaacg agcggttacc cggcagacct tttttgtctg actcaaacca
     9121 gtaattcata aacatatacg ggtcggcaaa atccggactc cagttgccaa tcgcgatgtc
     9181 gtaatcacct ttacccactc tgtcgcgcat ggtggcgttc gccagctttt ccagcttcac
     9241 attgatgccc agtttgttga gactggattg tgtcgccaga gcgatcggtt cccagttcgg
     9301 atcattatca gaatagagaa acgtcaggct ggtgggtttg ctcgtcactt tatcccattc
     9361 ggctttggct ttcgtttcgt catggttgta ttgcattgcc gtcgcatcgt atccccacat
     9421 gccttccgga atcgggccgc gcatttgttt tccgttacca ctcagaatgc cgttaaccat
     9481 gccctgataa tcggtagacc aggaaatggc ccgacgcaga tccgcctgat taagtggcgc
     9541 tttgctgtta ttcagataca gataggtgac gcgcagtgac ggatactctg ccacattgac
     9601 tttattttcc tgcttcaggg cgttgagttg atccaccggc agcgcatcgg caatgtcaat
     9661 gtcgccacgg gagagctgca ggcgacggga ggcactttca ccgataattt ttaccgatac
     9721 tcgtttaaag ttcggtttat tgccggggta atgcggattt ggcaccagaa ctaattgctg
     9781 acctttttgc cagcttttca gcataaacgg tccggagcca gcggtatttt gcgcgaggaa
     9841 accacgggca tcatccgccg catgctcctt taagaccgcc ggattgataa tggatgcacc
     9901 gtcattcgcc agcgtgtaga ggaacggtgc gaatgggtgg ctaagggtaa acttcaccgt
     9961 atgttcgtcg ggagcatcaa tctttaaatc tttgggaaat gcttctgctg gcccctggcc
    10021 gatttttagt aaccgctcaa aagagagttt tacggcttcg gcagtgacag gtgtgccatc
    10081 ggcaaattta gcattgtcct tcagggtgaa cgtccactct ttttgatcgt cagacgcttt
    10141 ccagctacta gccagatcgc cttcaacgtc ggttgagcct ttatcaccgt ccgttttgta
    10201 ctgaaccagt cgctgataag acgggtaggt cactgtccag tcgttattat caattgtcac
    10261 cgctgggtcg agggtttgtg ggtcggcagc tttaccgatc accagcatat ctttcggtac
    10321 ggcggcgtgc gcaaccggca ttgttgtggc aaggacgatc gcgagcaatg taggacgaaa
    10381 caaaatcgat ctcttcatga cagtttcctg attagctggc gtgctgtgtg ccaggggata
    10441 taaaacaggt gaattgatca gcgagtagtg ggtaactcgc tgccaggggt aattcgaagt
    10501 gccaccattc gctcgggata ccgacaaagc caccgccagt cataatcgca ttcagcaaca
    10561 ggcgattgcg ctgagcggcg ggcgggacgg aagggtgata agcatgggaa cgttcgtgca
    10621 tttcatcgaa ccctgcaccc atatcgagga tgttcccgtg ctcatcacga agcgtcaggt
    10681 cgatcgccgt gccacggctg tgattagaac cgaccgtcac atcaacaaca tattgcgggt
    10741 ctgggcaggc ttgccacaac atcgcctgtg cttgttgtgg gcgatacgca tcgtaaatca
    10801 ccagttgtaa gcctgacagc tgggcgatac tgatactttt cgccagcgca gtaatcgcat
    10861 ccttgtgtaa cagacaacgc gcttgctgat aaatagcttt acctgtgatg ttatcagcgc
    10921 aggcgtattt caattcgatc tccagatcag ggaagatcac ggctaaatca accagttcgg
    10981 tggtatccga cataaacgat tcctgtataa gacaaaaatt gctgcgcttt ccgcttatgc
    11041 agatctcatg ccacgccggg ataagcgcca gaatgctggc ttaaagttat attttttaac
    11101 ctttgatcaa catttgtgca gcgtagtgca gttttggtgc aagagaggaa gttaaggaag
    11161 gaatctcccg gaatcgtagc tgaaatcaca gtatttaagt gacagtgtca cgttaaatga
    11221 aaacccgcgt gtgcgggcga gaggaatttg tcagattttc agcggtaaca tactgctcat
    11281 ccagcctgga atttcttcgg cgggcagggg gcgggaaaag aaatatccct gaataacgcg
    11341 acagtggatc ttgcgtagca tctcaaattg ctctttggtt tcgacgcctt ccgccacgac
    11401 ggttaaatta aggctttgcc caatgctggt aatggcttca agtaaggcaa ggatgcgttt
    11461 ttcggtcaga caacgatcga caaaactttt gtcaattttg atttccgtta ccggaagact
    11521 gactaagcgg gataatccgg aaaagcccgt gccaaaatca tctaccgata aaccgatgcc
    11581 catatcgcgc aggatctgaa tgcgtttaaa gatttcggta tcgtgttcca tcatcatgct
    11641 ttccgtgatt tccaccgtca gctggtggcc gtcgataccc caggcttgca ttgcatcaga
    11701 cacctgatta ggcagctgat tactgcgaaa gtgcagcgcc gacaagttaa cggataacgc
    11761 cgggatatga atattctggc tacgccattc tgctaactga cggcaagctt ccgcgatgac
    11821 ccagcgccca atattttcga tttcaccgat ctcttctgcg agaggaataa accgtgaagg
    11881 gggcacatga ccatgcaggg gatcgtgcca gcgagcaagg gcttcgatgc cgtacagttc
    11941 acccgtttct gcgaagattt gcggctggta aaccagtttc agttggttat tgctaattgc
    12001 ttctttcagc gccgcgccta aaaccaaacg ctcttttacc atttcgttca tcgccgggct
    12061 gaagaactgc cagccgttac cgccattttt gcgaataaaa tccattgcat tgtgagcagt
    12121 ggagagcagg taatcgcggt ttttacccac gtcgtagctg atgccaatgc tcaaggtgag
    12181 cgggaatggt ttatcgtcaa tcattatcgg cttgctgacc acattccgta gctcatcggc
    12241 gatctgcgta atgttactga cgtcgttttc caggctcacg aggacaaact gcgtaccttc
    12301 gatacgacag agatattgat ccggtttgag tttttcacga aagcgattga ccacttccag
    12361 caatgcctga tcggcccacg catagccaag gctatcaatc acatcctgaa tatggtcaac
    12421 accgatgaga tacaccaccg gagagacggc tttgtcgacc aggtcatcga ggtaattgtg
    12481 caggttattg cgatttggca gaccggtcat cggatcaaat tggatgagtt gttcaatatg
    12541 ctgacggctt ttttcctgtt ccagcgccag cgcggccata tgctggctga tatctgccac
    12601 gcgttcgata aaggcgctgg tttctgctcc tgacgaggtt ttaatttgca ggatccccgc
    12661 aggcgcacca tcacgctgac gaatggtcgc tgaccagctt tgcgcatttt gaacttctgc
    12721 accgtgcgaa gatgacgccc agtgtatcgg catcccgttg cgcagcgcga acagcgaaac
    12781 atgcgattcg ttgagtacag attcgatgtt acgacaaatg atttccccca tttcatgaaa
    12841 tggcgggctg ctgcacatgg cggcgagaat attgccttca agctggcgga tttgcctttc
    12901 ttcggtgata tccgagaatg tcattaccag gttctgcaga tgcgcgagca cgtcataaac
    12961 cgggctgata gaggctttaa tccagatttt ttcaccggtg cgcgtcaaca gcagaaattc
    13021 gtcctgatcg cgggcggttt tccatagcaa ctgctgtaaa cgaatacggt tatcagcagg
    13081 gaattctgga atgttcagga gtgtatcggg ctgcataccg ctggcttcgt taatgcagta
    13141 accaaacatt tcggtaaatg cgcgattgca ctgcacaata tggcgttccg gatcgaggac
    13201 aatcaccggt cggtcgagat ggtcaacggc aataatcaat tgtcgggtct gttctttttg
    13261 cgccatttct acgctggcat cccgtaccag cgccaggtaa taaactttcc cctcggcgct
    13321 cactttcgat agcgcaaaac gggtccagat tttactgccg tcttttttct ccagctgcag
    13381 ctcccgactc atcccctcga cgcgagcttt accaccttca cggttgtgac gaatgtattg
    13441 aggatgcgca ggacgcaaat cccgcggaat cagcatatca atgttattgc caatgacttc
    13501 ttcacgtttg tacccccaga gcttctctgc ggcggggttg aaaaacatca cttcatcatt
    13561 ttcgttaatt aacaccgcgc ccatcatatt ttgctcaagg gcggggaaaa aaatgccatc
    13621 ggcggcatta tccgcatcgg ttagcttcat gattacctct gcatcctggc gcatctaaag
    13681 actggcttta cagagttcaa cacgggttct acctcgtctt ttggcgatat acagagcttc
    13741 atcggctatt tgaatgaggc gctcatagtc aggatgacca ttaaacatgg cggcaccgat
    13801 ggaaagtgag agggcaatat cctcgccgtt tgcggcttta aaataatgtc gacctttatg
    13861 gttaaaccgc aggccaaaat caaccaaaca caacgtgcga gatcgactaa ctgcaccata
    13921 ttctcctgaa atatgaagat atactgaaaa gaaataagcg acttaggaca gtttcaatct
    13981 acgctactgt tcttgagaag agtatagccc atcgtaatta tttttcggtg acagcgaata
    14041 tcgtatgttt tttcatattc atacattttt attaggggat ttatgactgt ttaactaagt
    14101 gtggttaatt gcgtagttaa tttgacttaa gtaagcatga ttactagtgg gatatttcaa
    14161 gagggtaaca agccgggggg aaagcaccgg cttgttacaa agtaagaatg ggagtttaac
    14221 tgccccaacg actttgcaga tagctgaccg cttgttgagt ctgtggctta ttcagatagt
    14281 cctcacggaa caagattgtg ccgctaattt ctggcacggc atcgttaaga tcgagctgct
    14341 ttttcagttc cggtacgccg ccgttaatca tccagtctgg ctctatcttt gaaggttcac
    14401 ccactttata gaaggcgata ccgatataca ggcgggtcct ggtcggttta acgacatccg
    14461 cccaccattt tgccaaaacg tcataacgtg cggcactccg tgagaacggc cagtaaattt
    14521 ggggagcaat gtaatccagc aatccttgtt ccacccatcg acgggtgtca gcgtaggatt
    14581 cgtcataggc tgccgcgcct cgggtatcgg aaccgagcgg atcgtgtgat cggttacgcc
    14641 acacgcctgc cgggctaacg ccaaattcga cttccggctt aatgctttta atagtgtgcg
    14701 atacctttgc aattaactgc tgagtattgt tgcgccgcca gtctgctttt gacgcaaatg
    14761 cgcctccgta tttgcggtac gtttcgttat catttagccg tgaacccggt gactcagtat
    14821 agaaatagtc gtcaaactgc acgccatcta ccggatagcg ggaaaccact tccgcgacta
    14881 ttgatgtgat ccagtcctga acttcaggga tgcccgggtc gaggacaaag cgatcgccgg
    14941 acgttctgat ccagtcgcgg tgttgcacat agacgctcgc tggttgttga gacagagtgc
    15001 tattcagttc cctgatagta ccgggcttcg tattaaccga tacgcgatag gggttaaacc
    15061 aggcgtgtac tttcatccca cgcttgtggg cttcgtcgag cataaattgc agcggatcgt
    15121 aacctggatt ttcaccaatc tttccggtca taagatcgga ccacggcaaa atttttgatg
    15181 gccacagggc ggtaccgtcc ggcttgacct ggaaaaagac cgtgttgatg ccgagacgtt
    15241 gcagatgatc cagtttgtcg accatcgcct gttgttgtac acgggcccgg ctggtggggt
    15301 tactaatgtt aaccgaggaa accggtggcc agtcgaggcg agacactgtg gccagccaga
    15361 tgccacgcat cggttgtgac gattgttgcg tcgtagctgg tggcttcgaa cctgttggtg
    15421 gtgtcaccat tgactctgga ggcgtgcttt tgcaactaca cagtaaaagt gccagtgcaa
    15481 ccagtatcgc tggtcttcta atcgctaatt tctcgtttcg ggagcagata tccatatgtg
    15541 ctggtttccg gtaaacagat gtgcgctcat tctcgtactt atcccggtta agtcaatacg
    15601 acaacaagca cgaaaaaggg agcgatgaat tatcgctccc ttgtcttata accattcaga
    15661 catggttagt gtttcttgtc attcatcaca atatagtgtg gtgaacgtgc acgcgggtgc
    15721 aggaagaagt gaccttttgg tgcgttctga ctgttgattg gctccagagt gacgccggta
    15781 ttcgctttgc ttttacgatc atgaacagca tagagaataa agggcagggc aagtaccacc
    15841 aggaaactca cgaccagtaa ttcaacatac atatcggtag agtcaccctg gatgttatcc
    15901 ggcggcagga aggaaacaat aaacgccata attgaagtca gcagaccgac aatagccacg
    15961 accagtttca cccctttacc accagggata ttaaatgtgc gttttaagtc aggatgttta
    16021 agaaccaaca caatgtagcc aataaacagc atgaaataag cacacagata aatcaccacc
    16081 gtcagcgcca gtgcgatcag gaaggacatg ttgttaccgc caccggtatt ggtgaggatg
    16141 atcaacgcga tagaagtaat caccaactgc gaaatgacca gcgttaccgg tacgccattt
    16201 ttgttcattt tagcgaatgc cgctggcagc aggtttttct gcgctgtgac atacatcccg
    16261 cgagaaggac caacaatcca ggaagcgatt tccgccagaa cacccagcaa cagcagtgcg
    16321 gagatcacgc gaaccgtcca ctcaatttcc ggtgccacat gtgacatcag aacggtaaag
    16381 gtttgcatta cccctgcgga gaggttgatt tcattacccg gaatgaccat cgcaatagac
    16441 aaaccgccaa cagagcttaa gcagattgcc gccaccatca gcagtaacat agccagtgga
    16501 tagtcgcgcc cgggattgct catttcattg acgtgggttg cggaagcttc aacgcccata
    16561 taactcaaaa tgaaggcaac aaatacaacc agggtgccca ctttagagaa gtcagggaag
    16621 aaggtcttcg aatccatttc gatagcaacg ggggcaccgg agtgcagata aatagccgct
    16681 aatgcgatca aaataaatgc aggtaacagg ataccggcga agaagccaac tttagcaatt
    16741 cgcgccgtgt atttcgtgcc accaaactgc gttaatgcca gcgcccaaag aatgatgagt
    16801 gctgcaatag ttttggtaat ggggtcttca ttcagcgctg gccatttcag gatgtaggag
    16861 agtgccccta acacgaaata gagcatcgga ataaaaccaa tggcgatttg cagatagcca
    16921 aatgagatcg ctgcaaatcc ccatctcggc cccagagtat ttgataccca ggcgaagacg
    16981 ccaccttctt cccagccgtc gacggtagcc atttccgcag cacaaagtcc cacgggaata
    17041 aaccataaaa tcccgcctaa tagcaggaag aagactaatg aaaagcccga tgttgcgaag
    17101 gtagggtatt cataaacagc cattaccatc gatgccgtta tggcaaaaaa tccgagtaat
    17161 gtgagctgct tagctttacc tgtctgtact gatgtagcca tattatcccc ctaaaacggt
    17221 attcctgtcg gaaccgcacc tgttttgttc tgaagcgtat tcagaacaat attttccgtt
    17281 gctaatgcca gtgaacagac tttggaaatt gtcccgaaac ggcttcgttt cgggacaccg
    17341 ttaccgttaa acgttatcag gtgtgtttaa agctgttctg ctgggcaata ccctgcagtt
    17401 tcgggtggtc gctgagatat ttcagggagg ctttgtagtc ttccagcaac agttcagcaa
    17461 agtccatttc gaagccgcga cggcacataa tgcgcatcac cacgatgtcg gtggcttcac
    17521 cgcctagagt gaaagccgga acctgccagc cgcgcagacg caaacgttca gagaggtcat
    17581 acagggtgta tcccggatct tcaccatctt tcagtttgaa gcaaaccgcc gggatgcctt
    17641 cgtccgggcg acccgtacag atgaactcat acggccccag tttggcgatt tcatccgcca
    17701 gataagc
//
LOCUS       DAZGTJ010000097        17404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000097 DAZGTJ010000000
VERSION     DAZGTJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.097"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..2285)
                     /locus_tag="ACHK8E_004080"
     CDS             complement(<1..2285)
                     /locus_tag="ACHK8E_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFP0346281.1"
                     /translation="MLVRFSGHNNGAKEYLEEGKKNGRELTRDELDTRITLYGDLELT
                     QKIYQSIPDNDQERYVSITHSYFEDDISVDVMKDIFYEYKNFLMCAYRDDEFNIYAEA
                     HIPKIKYSEDKKTGELIERKPHVHIIIPEVNLLSGNLINPRGDYRVNEKYFEAVQEYI
                     NQKYNLISPRERVRANPKNVSDILSRYKGDDFRSKNREFKEQLVRDIVEKNILSRKDF
                     YTHVSSFGDIRIRNQGKENEYIAVKLPGDKKFTNLKETIFNDDFIVRRELKKPPLEKS
                     VIQQLLHEWPQRAMELKYIDKANSDFRKKYYSSSQEEKIKILAQQETAFYQKYGERYD
                     VFPAEWKTNHKSGINEAQTRGTTFSPNGLQSMSGGEMATGWQTGNTGSSLFLSTDALV
                     FMGQSQSSGRDSGLRCDVHSGGRGRSGNQRGSGQPAITGTNATVHSRRSTRTQYGNRY
                     AGGLLAGDVSIPVYARNKPGGATIQDVIRRSEILLRPPPGKSNISVNRFNGDIPQMGK
                     NAAYVAAHFVRQYEQNKILPNQRQALWDIRQLYQSTRSFIWQDERLTRKERSQYLSVL
                     AFEHVKARQKIINSSQQENTMASEDIRKLIKKKPRLSNNEITGKDERKIPPAGKRFSR
                     IVHTLKNRIEGEDKENSYAKSVSAADLYTRKSKLSKNVHYLDKNSNRTLFVDTGNAIA
                     IRKDGMSPAALGIALELAKERFGSTLTIKGNEEFRNQVIDIVARNHPDLHFSDKAMNR
                     QLEERRAEIAAERDGMTIEGVS"
     gene            complement(2275..2631)
                     /locus_tag="ACHK8E_004081"
     CDS             complement(2275..2631)
                     /locus_tag="ACHK8E_004081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFP0346282.1"
                     /translation="MVTKKKDKINRDVVISLRLTEKEFGDFVDDIKKSQLSRSAFFRK
                     LILEKSSSVVINVNNTAPLVYHFNKAGNNLNQLAYKVNSAFLMGRISESLYVKVLNCL
                     VDIRELLMTGVNNNAR"
     gene            complement(3024..3653)
                     /locus_tag="ACHK8E_004082"
     CDS             complement(3024..3653)
                     /locus_tag="ACHK8E_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346283.1"
                     /translation="MSGNATNEIILAKINDILVDFNELNNQINNFPEKFSGKIDAINF
                     AIKQTPDALEESLKKLFSAAEELEKTVQKSTDECRVSMRAYAINELDNLKVKISEAIN
                     ESIKDSIDMPLDQAKERIVQINKMLQNSTGVEMSTRVAFGIGGLLGLSFLSLAMCISA
                     GYLYFQERSKAERYHNAYREQQIVIERLPPETQRQFRDELGRYLTRNQK"
     gene            complement(3663..4364)
                     /gene="stbB"
                     /locus_tag="ACHK8E_004083"
     CDS             complement(3663..4364)
                     /gene="stbB"
                     /locus_tag="ACHK8E_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HFP0346284.1"
                     /translation="MILKIVVLNNSGNVGKSTLCRSFLSPRIAGSKIINIESINTDGS
                     TSEKHRASDFIEIIKIIDSLPCVIVDVGSSNIEVFMNKLKSYDGAHEDFDYFIIPVTP
                     QNKQQQDTLVTISHLYDLNVPPEKIKIVFNQVDSSRNFDKQFSVMLESEIFKELGIVN
                     YSMVPDTELFRYLSDMGKDINSVLNDDRDFRTLMRETDDPDILELLSTERAIKRLATG
                     INKELDIAFNHLEII"
     gene            complement(4361..4783)
                     /locus_tag="ACHK8E_004084"
     CDS             complement(4361..4783)
                     /locus_tag="ACHK8E_004084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346285.1"
                     /translation="MEDWRTKPRTGASQFAGYEEEIRLLKEKKYTNKQIYDAISQKGI
                     TIGQKMFYIYMRRMFNDELYIKPSASKNKKEKTVLSDNIIKETTAWNNVNIRSLRLIK
                     DLESYGLTPEIVREWREPNEMAVRQHLTVLISKKGIIK"
     gene            complement(5052..5429)
                     /locus_tag="ACHK8E_004085"
     CDS             complement(5052..5429)
                     /locus_tag="ACHK8E_004085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346286.1"
                     /translation="MIKTLFDPETMSREDMLREIQLLRDSRDLALELVFGYPETKAAE
                     QIELNDDEITLLKVKLPEHHFQWLTGDGDEALTVGRLGDFADLADDFSVLAGEIDPDL
                     EEPQEEVISRGPCGVVFCLIEPD"
     gene            complement(5522..5986)
                     /locus_tag="ACHK8E_004086"
     CDS             complement(5522..5986)
                     /locus_tag="ACHK8E_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346287.1"
                     /translation="MKKVNVVGYTELIFGAISEKTEIKSKKLKRRCNNLIEKETPSRI
                     FKFTNFLNCGVQHLFVLHRMKTNIPYKTNDDMKKSTGGIITGSKFIGLIIVDLEEEKI
                     FVYQYSLNNRIKRKFRAYMKIKKDSPVKAITQFTKVNIWYWDEMLKQLMNKL"
     gene            complement(6133..6455)
                     /locus_tag="ACHK8E_004087"
                     /pseudo
     CDS             complement(6133..6455)
                     /locus_tag="ACHK8E_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6566..6793)
                     /locus_tag="ACHK8E_004088"
     CDS             complement(6566..6793)
                     /locus_tag="ACHK8E_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0346288.1"
                     /translation="MEIENTPENIKRLRKKIGLTQKECAEMFGMTARSWRRKEEPTGT
                     VSGSKLSPVEFEYILLLAGEHPEYVLSKRDA"
     gene            complement(6783..7043)
                     /locus_tag="ACHK8E_004089"
     CDS             complement(6783..7043)
                     /locus_tag="ACHK8E_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346289.1"
                     /translation="MKMRKGDRQYYLNKEGDTFRLVKRVKTFSKSATLGKTKATVKTV
                     ADIIFREEEFGSIDFTQDGLREGDKEIISIMLKNIKRDENGN"
     gene            complement(7234..9495)
                     /locus_tag="ACHK8E_004090"
     CDS             complement(7234..9495)
                     /locus_tag="ACHK8E_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFP0346290.1"
                     /translation="MRLWIAEKPELGKLIAQVLGNPQYFDGYIVCNNDTVSWAIGHLL
                     KLIPPELKTPEYKSWSKNTLPLQLRPLQYIVIPKTEKQFNVLSGLITRADMIIHAGDP
                     DEEGQLLIEEILSYCGNKAPVKRVLINDLNKDAVRQAMRNLQDNRAFYGLYQKALARS
                     AGDYLFGLNMTRAYTIAAREKGHNEILSVGRVQTPVLGLVVRRYEENRDYKKYDYWCV
                     QAEFIDTDTIISPMLVPPEDAPVDDKGRIISEQYCDEVIRSCKGQQASVSLAESEIKS
                     RRPPLPFSLLDLQVKMNRQYGFTAEKVLEITQKLREEYRAITYNRSDCRYLSEEQFSQ
                     SPATLAMLSSFFPELGKIFSHIDSGRKSRAFNDKKISAHTAIIPTPTPTGLDQMNEDE
                     MRVYKAIVAQYLVQFMPDKTFDSAVVEFNINGFLFRQRAIRPVSPGWSILLADRDEET
                     LPDCKLFDAITSLTKGQQLVCSSVNSRKETSTPPPLYTEASLLEDLRRVARYVEDPRI
                     RQLLLDRDEGKEEERGGIGTPATRGHILNVLQVRGFIAVKNKKVVPTELGLSFIHALP
                     EVMTRPDMTALWHEQQSMIETGEISIDDFLDELEQFILQQVSDVDISGLAVAEYQCTC
                     GGRYVRRRTDKGFFWGCSKYPDCRNTVPDRGGVPDFSRSEFERKVRCPVCGSGMKISP
                     KAYNCSNERCGFRLWGTQFGKELTLAQATDLLMKGHTREIKGLKKKDGAKFDAVLNLN
                     RDGSVVPTFKKKK"
     gene            complement(9536..9769)
                     /locus_tag="ACHK8E_004091"
     CDS             complement(9536..9769)
                     /locus_tag="ACHK8E_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP0346291.1"
                     /translation="MENKYRKYPKSSKQEYLFQLRKCSKLSTLDKIIQRKAYTMNDID
                     FVEFLTAADHRLAEIVTGQLFDKIPKNVWKLIY"
     gene            complement(9819..10175)
                     /gene="ssb"
                     /locus_tag="ACHK8E_004092"
                     /pseudo
     CDS             complement(9819..10175)
                     /gene="ssb"
                     /locus_tag="ACHK8E_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(10187..10723)
                     /locus_tag="ACHK8E_004093"
     CDS             complement(10187..10723)
                     /locus_tag="ACHK8E_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346292.1"
                     /translation="MGKLTLVEQALRLHKEYYGDECFGTIVKNYDYYPKVAEIFINRK
                     NKNTYSNLDIEPVASSGVVHTGSNPVMVKVGRTKDAASCAKMYLPEGININIKELGAV
                     LDRTTPAEIKEIINISIGMIRGTKKELSTEIIKNARAFLKGSLDLSIQEAEEDIDERE
                     DIYRQNGAQPDYLSYLED"
     gene            complement(10741..11253)
                     /locus_tag="ACHK8E_004094"
     CDS             complement(10741..11253)
                     /locus_tag="ACHK8E_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0346293.1"
                     /translation="MKITLTIFIFLLTAFQAANAGGIPGALAGILSTLNLEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGRVFTTNGTDASRFMVQSGAAWGYERYNTDKSLPALQREAQEQKRGLWADTNP
                     VPPWEWRKNN"
     gene            complement(11332..11907)
                     /locus_tag="ACHK8E_004095"
     CDS             complement(11332..11907)
                     /locus_tag="ACHK8E_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0346294.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKEYPVKELPDIDESAHPF
                     YVCYGGYLLNPSVSDSDISRKIKDQTGFWLAAIDDTHMDCHSIAYYDIHTLPLISCGR
                     QKIVPFAALIKADRCIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCEHR
                     FRQPVGEDNWKQAISEECAIRYANRLIQCEG"
     gene            complement(12024..12230)
                     /locus_tag="ACHK8E_004096"
     CDS             complement(12024..12230)
                     /locus_tag="ACHK8E_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346295.1"
                     /translation="MSEIRFVTLSSDTDINKIIQEGLTQKDATTTIVLGCASEGKEAP
                     LRLIETCFRTIPKHISIEEINEIK"
     gene            complement(12243..12632)
                     /locus_tag="ACHK8E_004097"
     CDS             complement(12243..12632)
                     /locus_tag="ACHK8E_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346296.1"
                     /translation="MDKSDEEKIGYILDEHERIFRKQASLYTVLGILNLIICLLCVVA
                     TFCVSVILPESHLKHLLFSCTILCMVCSFIIYYYTRHMVLNRQELLRLMRLTEGAPDV
                     KQELLNRLLSGKILTYWDEKDIRRLIF"
     gene            complement(12760..13071)
                     /locus_tag="ACHK8E_004098"
     CDS             complement(12760..13071)
                     /locus_tag="ACHK8E_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFP0346297.1"
                     /translation="MKYSVIAVIILFLLSSKSYAEEVSIDSGDPCTVFMCMAGKVYGE
                     NSSECKGPTKKFFNIIKKKKGRIRWSKTFDARKAFLMNCPGADPAHVSKIMSKFGRKL
                     F"
     gene            complement(13096..14724)
                     /locus_tag="ACHK8E_004099"
     CDS             complement(13096..14724)
                     /locus_tag="ACHK8E_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFP0346298.1"
                     /translation="MGKQKIPDGWVLENGKLIQKRVTHYPPIIFGKDPYSNNLLVSYG
                     QTYLMLAAPPGSGKGVGIVIPNLLLYPHSVVVNDCKFENWDLTAGFRKACGQKVVRFS
                     PERLETHHWNPLKVINQDPLYRLGDVRTLASSLYVPDNMKNASWFSKAADVFTAIVLY
                     LIETPSLPLSLPQCYEIASLGTRIGEWAQRVIQARVGTPDALSEETVRELGVIISESK
                     GREFSTLMSFVTGRLSLYGEKLVALALTESDNEEENIDFSKLREEPTSIYFCVTEGAL
                     QKFGPLMNLFFSQAIRENSKILPEQGGNLEDGSLRLKYQVLFLEDEFAVMGRIKVMET
                     APALSRGAGLRYIIVFQNKSQVCSDDCYGPAGGKAVMDPFHIEVVFAPGDISTAEEYS
                     KRLGTYTERVASSSRNISEGQRRSRGRNVTLHARPLMLPQEVNELPYPEELVFMQPTK
                     WNPALKGRVKKIMWYKEPELTARVDPVRYPVPDIPAGNISKLKGLVVPISQYITDAVK
                     ISSPEGDYLNEEQKKRLNIPQVESERESYEDNDY"
     gene            complement(14724..15086)
                     /locus_tag="ACHK8E_004100"
     CDS             complement(14724..15086)
                     /locus_tag="ACHK8E_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346299.1"
                     /translation="MEKLKLFIVIIALCCLFFVIGLYAGGWMWLELSGVDSSPSLMTL
                     ISQGGLPALNDRARMLLPWAWCVTVAITFFPAGVSLLAMTHGADGNRKDLHGNARFAG
                     KRELRRFWYEGPEKEDRN"
     gene            complement(15098..16147)
                     /gene="virB11"
                     /locus_tag="ACHK8E_004101"
     CDS             complement(15098..16147)
                     /gene="virB11"
                     /locus_tag="ACHK8E_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFP0346300.1"
                     /translation="MNTEFDLDKSELVLGWLDQSGVTERLSRQGVTEVAINRPFEIWT
                     ETSKDGWQREDAPWLTLNMCEHLAETLAVYNNRILGVKSPIKTVELPGGERGQIVIPP
                     ACEKSTVSMTFRKPSLQRFSLENYDSSGRFDNANRVRGRSKELLDWQKELKRLNAARE
                     YVEFFRLAVRHKLNIIIFGGTGSGKTTFAKAIVDVYPSNRRLLTIEELNEMKLPYHLN
                     HVHLLYGDYVTPKDLVACCMRMKGDHILLAELTGDETWDFMELLNTGHPGTVTTAHAN
                     SSLHGYARISGLIKQSAVGAGLDISYIDRRVRTSFDVVMYMEKTEILDITYEPEVQLA
                     LLNGASMESALSFAS"
     gene            complement(16358..>17404)
                     /locus_tag="ACHK8E_004102"
     CDS             complement(16358..>17404)
                     /locus_tag="ACHK8E_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFP0346301.1"
                     /translation="AFMEAHLPQDRDKTESDSRTVQSSGKQESNGPVSDKKNTQTSGG
                     QSTVNPVTPVTDIRVPETLFSPVARFDSSGISQGVGGSSDGVTSSSGYDVSAEEARLR
                     EFANADPAEIVNRALQSQADTVNGGISSDMRSNILGDLSSSRQYATAKAYKSPPRKYL
                     LKRRTNFQCVLYTAVKTDHSGFVGCRLTKPIFSADGSVILAEAGAELNGEQKIEVKQG
                     QSTVFTSWSELETTAGTEGGIRADLNGLGTGPMGSSGTDAYIDNHYGQRFGGAVMLSF
                     IQDAIATAANAAQKNKSTYSFDNSEDNAESMAEKALENSINIPPTGYVLPGTVINVIV
                     AQDIDFSSVYTVKY"
BASE COUNT         4868 a         4064 c         2824 g         5648 t
ORIGIN      
        1 gaaacgcctt ctattgtcat cccatcacgt tcagcggcaa tttctgcccg tcgttcttca
       61 agttgccgat tcatcgcttt gtcagaaaaa tgaagatctg ggtgatttct ggcgacaata
      121 tcaataacct ggttacgaaa ttcttcattc cccttgatag tcagtgtact gccaaaacgt
      181 tctttggcca gttcaagagc aatccccagc gccgccggag acataccatc ttttcttata
      241 gcaatggcat ttcctgtatc aacgaataat gtcctgttac tgtttttatc aagataatgc
      301 acattcttac tgagtttcga ttttctggta tataaatccg ctgcactaac agactttgca
      361 tatgaattct ctttatcttc accttcaatt cggtttttca gtgtatgaac tattcttgaa
      421 aaacgttttc ctgccggtgg tatcttccgc tcgtctttcc ctgtaatttc gttatttgac
      481 aggcgtggtt ttttcttaat aagtttgcgg atatcctcac ttgccatagt attctcctgc
      541 tgactactat taatgatttt ctgacgcgct tttacatgct caaaagcgag tactgacaga
      601 tactgtgagc gttctttcct agttaatcgt tcatcctgcc agataaaact ccgggttgac
      661 tgatagagct gccgaatgtc ccacaatgcc tgacgttgat taggtaatat cttattttgt
      721 tcatattgac gaacaaaatg agcggctaca taagctgcgt ttttccccat ttggggtata
      781 tcaccattaa acctgttcac actgatattt gattttcctg gaggtggtcg taaaagaatt
      841 tcactacggc gaattacatc ctgtattgtt gcccctccgg gtttattacg cgcatacaca
      901 ggaatactca catccccggc cagtaatcct ccggcatatc gatttccgta ctgagttctt
      961 gttgaccgtc tggagtgaac ggtggcattc gtcccggtaa tagcgggttg tccgcttcct
     1021 cgttgattac cagatcgtcc tcttcctcct gaatgtacat cgcatcgcag tccagaatct
     1081 cttccgctgg attgcgactg tcccatgaaa acaagtgcat cagtggacag aaacaacgaa
     1141 ctcccggtgt ttccagtttg ccaaccagtt gccatttcgc caccggacat actttgcaag
     1201 ccattgggag agaaggtagt tcctctggtc tgagcttcat taataccgga cttatgattt
     1261 gttttccatt cggcagggaa tacgtcatat ctttctccgt acttctgata aaatgctgtt
     1321 tcttgttgtg caagaatttt tattttctcc tcctgcgagg atgaataata tttttttcgg
     1381 aaatcactgt tagccttatc aatgtactta agctccatag ctcgctgtgg ccattcatgc
     1441 aaaagctgct gaatgacaga tttttccagt ggaggtttct tcagttctcg tctcacaata
     1501 aagtcatcat taaatattgt ctccttcagg ttggtaaact ttttatcacc cggtaattta
     1561 acggcgatat attcattttc tttaccctga ttacgaattc ggatatcacc aaatgaagat
     1621 acatgtgtat aaaaatcttt tcttgataaa atattttttt caacaatatc cctgaccagt
     1681 tgttctttaa attctctgtt cttacttctg aaatcatccc ccttataacg agacaaaata
     1741 tcagacacat tctttggatt ggctctgaca cgctctcggg gagaaatcag attatatttt
     1801 tggtttatgt attcctgaac agcctcaaaa tatttttcat ttaccctgta atctcccctg
     1861 gggtttatta aattaccaga aagaagatta acctctggaa taataatatg aacatgtggt
     1921 tttctttcta tcaattcacc ggtcttctta tcttccgagt attttatttt tggaatatgt
     1981 gcctctgcat atatattaaa ttcatcatcc ctatacgcgc acataagaaa gtttttatac
     2041 tcataaaaaa tatctttcat cacatcaaca gaaatatcat cctcaaaata cgaatgtgta
     2101 attgaaacat aacgctcctg atcattatca ggtatagact gataaatttt ttgggttaat
     2161 tccaaatcac catataaagt aattctggta tccagctcat cacgagtcaa ttctcgccca
     2221 ttttttttac cctcttcaag atattctttt gctccattat tatgaccaga aaacctaacg
     2281 agcattatta ttaactcctg tcattaataa ctccctaata tcaacaagac aatttaatac
     2341 ttttacatat aaggattcag aaatacgccc catcaaaaaa gccgagttaa ccttataagc
     2401 aagctggtta agattattac cagctttatt aaagtgatat actaaaggag ccgtattatt
     2461 aacattaatt acgacagatg atgatttttc aagaatcagt tttctgaaaa atgcagaacg
     2521 actgagttga gattttttta tatcatcaac aaaatcgcca aattctttct ctgtcaatct
     2581 taaggatata acgacatccc tgttaatttt gtcctttttt ttagtgacca caggatacct
     2641 cctgtaaata attaacaatg ccaccgccag gtcgtccgtc ccctgacggg gtttcaaagg
     2701 ggcgtcccct ttggcacgtc gacgtatcaa aatgcgccag cattttgtat acagtcggtc
     2761 acgtgcctcc agaatttacc ctcaatctcc ccctgtaact gacagctaac taatgtccat
     2821 ctagcgtcca tctaacgtcc atctaatatc catctaacgt ccatctaaat caaagatggc
     2881 caactaagat ccatctaatg tccatctaat gtccatctaa cgtccatcta atatccatct
     2941 aacgtccatc taaatcaaag atggccaact aagatccatc taatgtccat ctaatgtcca
     3001 tctaacgtcc atctaacgcc tatttacttc tgattacggg taagataacg ccccaattca
     3061 tcccggaact ggcgctgcgt ttctggcggc aaacgttcta tcactatctg ttgttcacgg
     3121 tacgcattat gataacgctc tgctttgctc cgctcctgaa aatacagata tcccgctgat
     3181 atacacattg ctaaagaaag aaatgaaagc cccagcaatc caccaatacc aaatgctacc
     3241 cttgtactca tttctacccc ggtactattt tgtagcatct tatttatctg aacaatccgt
     3301 tctttcgcct gatctaacgg catatcaata gaatctttaa tggattcatt tatagcttct
     3361 gatattttca ctttcagatt atctaactca tttatagcat acgccctcat tgatacccga
     3421 cattcatctg tacttttctg cactgttttt tctaactctt ctgccgcaga aaataatttt
     3481 tttaatgatt cctctagcgc atcaggtgtc tgttttatgg caaagttaat tgcatctatt
     3541 ttccctgaaa acttttcagg aaagttattt atttgattgt ttaactcatt aaaatcgaca
     3601 agaatatcgt ttattttcgc caaaataatt tcatttgttg catttccaga catattcaca
     3661 ccttatataa tttcgagatg attaaatgca atatccagct ctttatttat acctgtagca
     3721 agacgcttta tagctctttc agtagacaat agttcaagta tatctggatc atcagtttcc
     3781 ctcattaatg ttctaaaatc cctgtcatca ttcaggacag aatttatatc tttccccata
     3841 tcagaaagat atctaaataa ctctgtatca ggaaccattg aataatttac tataccaagc
     3901 tctttaaata tttcactctc cagcattaca gaaaactgtt tatcaaagtt cctcgatgag
     3961 tcaacctgat taaaaacaat ctttattttt tccgggggaa catttaaatc atataaatgg
     4021 ctgatagtaa ctagtgtatc ttgctgttgt ttattttgtg gagtcacagg tataatgaaa
     4081 taatcaaaat cctcatgagc tccatcataa gatttcagtt tattcataaa aacctcaata
     4141 ttagatgaac caacatcaac aattacacac ggtaacgaat caattatttt tataatttca
     4201 ataaagtcag atgctctatg cttttcactt gtagaaccat ctgtattaat actttcaata
     4261 ttaataattt ttgatcctgc aattcttgga gataaaaaag atctacacaa agtagattta
     4321 ccaacattac cagagttatt caatacaaca attttcagaa tcattttatt attcctttct
     4381 tactaattaa tactgtcaag tgttgtctta cagccatctc atttggttct ctccactctc
     4441 gaacaatttc aggggtaaga ccgtaagact caagatcttt tattaatcgt aaactcctta
     4501 tattaacatt attccacgct gtagtttcct ttattatatt atcactcaaa acagtttttt
     4561 ctttcttatt ttttgatgca cttggtttta tatataattc gtcattaaac atgcgtctca
     4621 tatatatata aaacattttt tgtcctattg ttattccctt ctgggatatt gcgtcatata
     4681 tttgtttgtt cgtatatttc ttttccttta ataatcttat ttcctcttca tacccagcaa
     4741 attgagacgc tcctgttctt ggcttagtac gccaatcttc catttcatca tctccataaa
     4801 gttcaagcct gttactgtta caattgcttg tcttcgacca ttaaggaacg tgttacctga
     4861 tatatggttg tctggcgcat tctgcgccag acacaaacac gtctggcagg gggaggaggt
     4921 gttcctgtga tttatttaca tccgggcggc gtagccgcgt cacggcgaca ctttacagcc
     4981 aattatattt aacttgtttt tgtcgcctcc catgccgcag acgcggcatt tactgcacct
     5041 cacacacaag atcagtcagg ctcaatcaaa caaaatacga caccacaagg cccacgggat
     5101 ataacttctt cctgtggttc ttccaggtct ggatctatct ctccggccag aacgctaaaa
     5161 tcgtcagcca gatctgcaaa atcacctaat cgcccaactg ttagcgcctc atctccatct
     5221 cctgttaacc attgaaaatg gtgttccggt agtttcactt tcagaagggt tatttcatca
     5281 tcattcaatt caatttgttc tgcggccttt gtttctggat agccgaacac cagttcaagc
     5341 gccagatcac gtgagtcgcg cagtagctga atttccctaa gcatatcctc cctgctcatg
     5401 gtttctggat cgaacaaggt tttaatcata aatatatctc gtttaattta ttaatgcctg
     5461 tttttaacta tgctaacaca agtaataata cagagtaagg cgggtttccc cgccttgtct
     5521 gtcataattt attcataagt tgctttaaca tttcatccca ataccatatg tttacctttg
     5581 taaattgagt tattgctttt acaggactgt ctttttttat tttcatatat gccctgaatt
     5641 ttcttttgat tctgttattc aggctatatt gatacacaaa aattttttct tcttccagat
     5701 caacaatgat tagtcctata aatttactac ctgtaattat ccctccggta gacttcttca
     5761 tatcatcatt agttttatat gggatatttg ttttcattct gtggagtaca aataaatgtt
     5821 gtactccaca atttaaaaag tttgtaaatt tgaatattct gcttggtgtt tccttttcta
     5881 tcaggttatt acatcttctt ttaagttttt tactctttat ttctgttttt tcagatattg
     5941 caccaaaaat taattcagta tatccaacaa catttacttt cttcattttt aatctcctgc
     6001 cgctgtgcgg ctctggtgat tggggccatc ccctctcgat gtattaatag taggtcataa
     6061 acgacctatt tgcaagagtt ttttctcatt tttaaggaat aaaatagcag gagttccccc
     6121 ctgctataat ccttaagaat atgaagtaaa ttcgtgttct tcttctggta aagttttata
     6181 cgctatttcg aattcttcaa ttacaattac tggaacacga taacaatcaa gaactcgacg
     6241 ttttagtggc tctcctctga aatcattcaa caacggacgt ttatacccca tcatatttcc
     6301 ggtaaagtga acactggcac aaatctcacg aacagtcacc gtttttttcc gtgaacagaa
     6361 atgacctgat agaaattaac attcgtttgt tcgtatcccc atgaagacac aaatatgtca
     6421 ccaacacaaa ctgtaccatt tttaaaagtt aacattttta atctcctgcc gctgtgcggc
     6481 tttggtgatt ggggaccatc ccctttcgat gtattaataa taggtcataa acgacctatt
     6541 tgcaagttat ttttcttctt ttttgctagg catctcgttt agaaaggacg tattcaggat
     6601 gctccccagc cagtaacaga atgtattcaa actctacagg tgaaagtttt gatccgctta
     6661 ccgttccagt gggttcttct ttgcgccgcc atgaacgggc tgtcatcccg aacatctctg
     6721 cacattcttt ctgcgttaat ccaattttct ttcgcagacg cttgatattt tctggtgtat
     6781 tttcaatttc cattttcatc tctttttatg tttttaagca taatagaaat tatttcttta
     6841 tcgccctctc ttaagccgtc ttgagtaaaa tcaatagagc caaactcttc ttcacgaaaa
     6901 ataatgtctg ccactgtttt taccgttgct tttgttttcc ctaaagtcgc agacttagaa
     6961 aaagttttta cccgcttcac cagacgaaac gtatcacctt ctttattcag ataatattgc
     7021 ctgtcacctt ttctcatttt catatctata tgattctctc taagagaagc ctcaagcgga
     7081 gccgaacgac tcgtacactg tacgcatggt ttccctattg caacagattt aacatacttt
     7141 tccatcttca aactcctgcc gctgtgcggc tttggtgaca gagaactatc ccttctcgat
     7201 gtaacggtag gtcataatcg acctgtttgc aagttatttt ttctttttaa atgtcggtac
     7261 aacgcttcca tcacggttaa gattcagcac ggcatcaaac ttagcaccat cttttttctt
     7321 aagaccttta atttcccgcg tatggccttt catcaacaaa tctgtagcct gtgctaaggt
     7381 cagttcttta ccaaactgag taccccacaa tcggaatcca catcgttcat ttgaacagtt
     7441 ataggctttt ggtgatatct tcatcccgga accacatacc gggcatctga cctttctctc
     7501 aaactcgcta cgcgaaaaat caggcacgcc gcctctgtct ggaacagtat tccgacaatc
     7561 aggatactta ctgcatcccc aaaaaaaacc tttgtcggtt ctccgacgaa cataacggcc
     7621 accacacgta cactgatact ctgctacagc caggccagaa atatccacat cagacacctg
     7681 ttgcaaaata aactgttcaa gttcatcaag aaaatcgtcg atactgattt cccctgtctc
     7741 aatcatagat tgctgctcat gccatagagc cgtcatatct ggtcgcgtca tgacctccgg
     7801 taaagcatga ataaacgata agcctaactc tgtaggaacc acttttttat tcttgacagc
     7861 aataaaaccg cgaacctgaa gtacgttaag aatatgcccc cttgttgccg gtgttccaat
     7921 tccccctcgt tcctcctctt taccttcgtc tctgtccagt aaaagttgtc taatcctggg
     7981 atcttcgaca taacgggcca cacgccgtag atcctcaagc aatgatgctt ctgtatataa
     8041 tggtggcggt gtggatgtct cttttcgaga attcacacta ctgcaaacca attgttgacc
     8101 ttttgtaagg ctggttatgg catcaaataa cttacagtcc ggcaaggttt cttcatctct
     8161 gtctgctagt aatatactcc agccagggga aacaggccgt atcgctcgtt gccggaacag
     8221 aaatccattg atattaaatt caactaccgc agaatcaaaa gttttatcag gcataaactg
     8281 cacaagatac tgtgccacaa ttgccttata aaccctcatt tcatcttcgt tcatttgatc
     8341 cagcccggta ggcgttggtg tcggaataat tgctgtatga gcacttattt tcttatcatt
     8401 aaatgccctg ctttttcgcc cactatctat atgagaaaaa atttttccca gttcaggaaa
     8461 aaaagaagaa agcattgcta atgtcgccgg agactgagaa aactgttctt ctgagagata
     8521 acggcaatcc gaacggttat aagtgatagc cctgtattcc tcccttaatt tttgcgttat
     8581 ctccaacact ttttcagcag tgaatccata ctgacgattc attttcacct gaagatcaag
     8641 aagcgaaaat ggtaatggtg gtctcctgct tttaatttca ctctctgcaa gagaaacact
     8701 cgcttgttgt cccttacaac tacgaattac ttcatcacaa tattgctcgc tgataattct
     8761 acctttatcg tccacaggtg catcttccgg tggaacaagc atcggtgaaa ttattgtatc
     8821 agtatcaata aattcggcct gaacacacca ataatcatat ttcttataat ccctgttttc
     8881 ttcataccgc cgaacgacca ggccaagaac tggtgtctga acccgtccta ctgaaagtat
     8941 ttcattatgc cctttttctc tggctgcaat agtatatgct ctggtcatat ttaaaccaaa
     9001 aagataatca cctgcactac gggcaagagc tttctgataa agcccgtaaa acgctctgtt
     9061 atcctgtaaa ttacgcatcg cctgacgcac tgcatcttta ttaagatcat taatcagaac
     9121 ccgtttaacg ggagctttat ttccacaata agaaagtatc tcttcaataa gtaactgccc
     9181 ttcctcatct ggatcccctg catgaataat catatctgca cgagtaatta atccagacaa
     9241 aacattaaat tgtttctctg tttttgggat aacgatatat tgcaacggtc gtaattgcag
     9301 tggtaatgta tttttagacc agcttttata ttcaggagtt ttcaattcag gaggtattag
     9361 ctttaacaaa tgaccaatag cccaagaaac cgtatcattg ttgcaaacta tatacccatc
     9421 aaaatactgt gggtttccca gaacctgagc aataagcttc ccaagctctg gtttttcagc
     9481 tatccagagt cgcataagaa tatctccgct tgaaaagtac gactggatgt atgatttaat
     9541 aaatcagctt ccagacattt ttaggaattt tatcaaataa ttgtccagtt acgatttcag
     9601 ccaaccggtg atctgcggct gtcaaaaact ctacaaaatc aatatcattc attgtatatg
     9661 ccttacgctg aataatttta tcaagagtag aaagctttga gcacttcctg agctgaaaca
     9721 aatactcctg cttactactt tttgggtact tcctgtattt gttctccatt taagttattc
     9781 ctcaaacgga ctcaatatct tcaagactgt cctgtttttg atttagtatt attaccaagc
     9841 atttgcatag ttcccttctg atcaacaaca atctctgttg tataatgctc ctccccatct
     9901 ttatctgtcc attttctgta acgtaatgtt ccctcaatat agacctgcat tcctttacat
     9961 aaaaaatctc tggatatctc tgccagtttg ttataaataa caactcgatg ccattctgta
    10021 ttctctttta tttcaccaga ctgtttatct ctccacgttt cgcttgttgc tatacttaaa
    10081 ttagcaacgg taactccatt ctgcatacac cgaatctcag gatcttgtcc aagattacct
    10141 attaatgtaa ctttatttat acctttagca gccatttaat accccactaa tcctcaagat
    10201 aacttaaata atctggttgt gccccgttct gtctgtatat atcttccctt tcatcaatat
    10261 cctcctccgc ttcctgtata cttagatcaa gagatccttt taaaaatgca cgagcatttt
    10321 taattatttc tgttgataac tcttttttag ttccgcgaat cattccaatt gatatattta
    10381 ttatttcttt aatttcagca ggtgtagttc tgtcaagtac cgcgccaagc tctttaatat
    10441 tgatgtttat accttctggt aaatacatct ttgcacaact agcagcatct ttagtccttc
    10501 ccactttaac cattactgga ttacttcctg tatgaacaac tccagaagaa gctacaggtt
    10561 cgatatcaag atttgaatat gtatttttat ttttcctgtt tataaatatt tctgctactt
    10621 taggataata atcataattt tttacaatag tgccaaaaca ttcatcacca taatattctt
    10681 tatgaagccg caaagcttgc tctaccaaag ttaatttccc catatgttac ctttaataaa
    10741 tcagttgttt ttccgccatt cccacggtgg aaccggattt gtatcagccc ataaaccacg
    10801 cttttgttcc tgcgcttccc gttgcaaagc cggtaatgat ttatctgtgt tgtaacgttc
    10861 atatccccat gcagcgccag actgaaccat aaagcggctg gcatccgtgc cgttcgtcgt
    10921 gaacacacga ccgataatgc gtccgtagcg gtctgtctgc gtgtacgtca cagtaacagg
    10981 ctgtgctgca agcagagctt taagctgatt tgctgaccag cgcccgaaag cctgtttttt
    11041 ctccggtgca tcgatgtttg ccaggcgaat cctgacgggc tttttgtcct gtaaaacttc
    11101 gatggtatcg ccatcgagaa cacgaataac tttcccctgg atttccaggt ttaatgtaga
    11161 aagtattcct gctaaagcac caggtattcc acctgcattt gcagcctgaa aggcagtaag
    11221 cagaaaaata aatattgtta atgtaatttt cacaaaatac taaccccctc cggttgttca
    11281 actccgcaaa tcccatatta aaatcaggaa tctgcggagt ttatgatttg cttatccttc
    11341 acattgaatt aatctgtttg catagcgaat ggcacattct tctgatattg cctgtttcca
    11401 gttatcttca cctacaggtt gtctgaacct gtgttcacag ccattaaaag caaaaatccc
    11461 ctcgcgatta agtatgttgg ttgcaatatc ccactcttta atccttaaaa atgccgttac
    11521 tgcaaaacca gaatatgatg caattttcga aatgatgcat ctgtcagctt ttattaacgc
    11581 ggcaaaagga actatctttt gacgtccaca cgaaatcaga gggagtgtgt gaatatcata
    11641 ataagctatt gaatggcaat ccatgtgagt atcatcaata gctgccagcc agaatcctgt
    11701 ctggtccttt atttttctgc taatatcaga atctgaaaca ctaggattta ataaatatcc
    11761 accataacag acatagaatg ggtgagcaga ttcatcaata tcaggaagct cttttaccgg
    11821 atattctttc cagtccttca gaacaccgaa ctcaagagca tccagcaaaa actttcgttc
    11881 aaatccatca aacaatgcat attgcatata ctacccctca cagaatgcct gatttatata
    11941 actaatgcgc tatcaataga tggctgtctc ttaaatataa tgagattcga taataccaga
    12001 ataacaacga ctaccgtcat cattcatttt atttcattga tttcttcaat tgaaatatgc
    12061 tttggtatag tcctgaaaca agtctctatg agcctgagcg gtgcttcttt accctcactc
    12121 gcgcaaccca acacaatcgt agttgtagca tctttttgag taagaccttc ctgaataatc
    12181 ttgttaatgt cagtgtcaga tgacagagtt acaaatctga tttcgctcat atgattacct
    12241 cttcaaaaaa ttagacgtcg gatatctttt tcatcccaat aagtcagtat tttcccggat
    12301 agcagacggt taagcagttc ctgtttcaca tctggtgcgc cctctgtcag acgcataagt
    12361 cgaagcagtt cctgacggtt caggaccata tggcgggtgt aataataaat aataaaactg
    12421 cataccatac aaaggatagt acaggaaaat aacaggtgtt tcagatgtga ttccggcaat
    12481 ataacagaaa cacagaacgt ggcaacaaca caaagaagac atataattaa attgagtata
    12541 cccagtaccg tatataaaga agcctgtttt ctgaatatcc gctcatgttc atccagaata
    12601 taacctattt tttcctcatc tgatttatcc atatatttca ttcccatagt tgatgtggaa
    12661 atagttattc ctctccgcaa atctaggata aacatatgtc atctgcggag aatagttcaa
    12721 acgcctctag tttagttcta aagcctttga ttattttatt caaaaaagtt ttcttccaaa
    12781 cttactcata attttactga cgtgagcagg atctgcccca gggcaattca ttaaaaatgc
    12841 ttttctcgca tcaaaagttt tactccaccg aatacgccct ttcttctttt ttataatatt
    12901 aaagaatttt tttgttggtc ccttacattc agatgaattt tccccataaa cttttccagc
    12961 catacacata aagacagtac agggatcccc cgaatctata cttacctctt cagcatagct
    13021 tttactggat aacaggaaaa gaataatcac cgctatgaca gaatatttca tattaacccc
    13081 ctttttatta aaaattcagt aatcattatc ttcataagat tccctttctg attccacttg
    13141 tgggatgttt aatcgttttt tctgctcctc atttaaataa tcaccttctg gtgaagatat
    13201 cttaactgca tccgtaatat actgacttat tggtacaaca agtcctttaa gtttcgatat
    13261 attcccggct ggtatatcag gcacgggata acgaacagga tccactcttg ctgttaattc
    13321 aggttccttg taccacatta tttttttcac ccggcctttc aaggcgggat tccattttgt
    13381 tggctgcata aacaccagtt cttcaggata tggaagttca ttaacctcct gtggcaacat
    13441 taaaggccta gcatgcagtg taacattacg tcctcgagaa cgacgctgtc cttccgatat
    13501 attcctgcta ctggacgcaa ctcgttctgt gtacgttccc agtcgtttac tgtactcctc
    13561 cgctgtactg atatctccgg gggcaaatac aacctcaata tggaatggat ccattacagc
    13621 tttcccccct gcgggtccat aacagtcatc agagcaaacc tgacttttat tctgaaaaac
    13681 aattatatag cgtaatcctg cccccctgct tagtgccggg gctgtttcca taaccttgat
    13741 acggcccatc acagcaaatt catcttccag aaaaagcacc tgatatttca atctgagaga
    13801 accgtcctcc agatttcccc cttgctccgg tagtatttta ctgttttccc ttattgcctg
    13861 agaaaaaaac aaattcatta gtggaccaaa tttctgtaaa gctccctcgg taacacaaaa
    13921 ataaatgctg gttggttctt cccttaactt gctgaagtca atattttctt cctcgttatc
    13981 actctccgtc agcgccagcg ccaccagttt ttcgccgtac aagcttagac gtcccgtgac
    14041 gaatgacatc aacgttgaaa attccctgcc tttcgactcg gaaataatca cccccaactc
    14101 tcggacggtt tcttctgaca gtgcatctgg cgttcctaca cgagcctgaa taacacgctg
    14161 cgcccattct ccaatacgag ttcccaacga tgcaatttcg taacattgcg gtaaactcaa
    14221 tggcaacgat ggtgtttcga tcaggtataa aactatcgcg gtaaacacat ctgcggcttt
    14281 agaaaaccaa ctggcattct tcatgttatc aggtacatac agagatgagg ctagtgttcg
    14341 aacatctccc aagcgataca gcggatcctg atttattacc tttaacggat tccagtgatg
    14401 ggtttccagt cgctctggcg aaaaacgcac gactttctga ccacatgctt ttctgaaacc
    14461 cgctgtcaaa tcccagtttt caaatttaca gtcattcact acaacagaat gcggatacaa
    14521 tagtagatta gggatcacaa tccccacccc tttaccactt cccggcggag cggccaacat
    14581 cagataggtc tgtccatagg atacaagtaa attattactg tacggatctt taccaaaaat
    14641 aattggcgga taatgagtta ctcttttttg gattaacttt ccattttcca gcacccatcc
    14701 gtctggtatt ttttgttttc ccattaattt ctgtcctcct tctctggccc ttcataccag
    14761 aaacgacgca attcccgttt tccggcaaac ctagcattac catgtaggtc ctttctgttg
    14821 ccgtccgctc catgagtcat ggccaaaaga gaaacacctg ccgggaaaaa ggtaattgca
    14881 actgtcacac accacgccca gggaagtaac attctggccc gatcattcag tgctggcagt
    14941 cctccctgac ttatcagggt catcaaagat ggagaggagt caacacctga taattcaagc
    15001 cacatccatc ctccagcgta aagcccaata acaaaaaaca aacaacaaag agctattatc
    15061 acgataaata gtttgagttt ttccatacag cactacctca cgatgcaaaa gaaagagcac
    15121 tttccatgct ggcaccattc agtaatgcca gttgcacctc tggctcatac gtaatatcca
    15181 agatttctgt tttctccata tacatcacaa catcgaaaga cgttctgaca cgtcgatcga
    15241 tataagagat atccagcccg gcaccaaccg cagactgctt tattagtcca gagatacgag
    15301 cataaccatg taaactgcta ttagcatgcg ctgtcgtaac cgttcctgga tgcccggtat
    15361 tcagcagttc cataaaatcc caagtttcat cgcctgtcag ttctgccaga agaatatgat
    15421 cccccttcat gcgcatacaa cacgctacca ggtcttttgg tgtcacataa tctccataca
    15481 ataaatgtac gtgattaaga tgataaggca gcttcatttc attcaattct tcaatagtca
    15541 gtaaccgacg atttgacggg taaacatcaa caattgcttt agcaaaagtt gtttttcccg
    15601 aaccggttcc accaaaaata ataatattta gtttatgtct cacagccaga cggaaaaatt
    15661 caacatattc acgagcagca ttaagacgtt ttaattcctt ttgccagtcc agtaactctt
    15721 tacttcttcc tcttacacga ttggcattat cgaaacgtcc actggagtca taattctcga
    15781 gagagaaacg ttgaagagac ggtttacgaa acgtcatcga cacagtagat ttctcacagg
    15841 cgggaggaat aacaatttgt cctcgttcgc ctcctggtag ttccactgtt tttatcggtg
    15901 acttaacacc taatatacgg ttattataaa cagccagtgt ttcagccagg tgttcacaca
    15961 tattcagcgt taaccacggt gcatcttccc tttgccagcc atcttttgat gtctcggtcc
    16021 agatctcaaa aggtctgtta atcgctacct ccgtcactcc ctgacgggac agacgttctg
    16081 taacaccaga ctgatccagc caccccaaaa caagttctga cttatcgagg tcaaattcgg
    16141 tattcattaa tagacgccct ttttatttac acttttatga gttacttttc tatctcatta
    16201 atgaaatgat aaatgcagca acgatcatta tgattgacca ccgtaaattt attgggatac
    16261 gataaaaata aatacacacc catttaaaaa atcccatcca acctccttca agcaattact
    16321 ttaatatata gccacccgtt aaggcggcta tatatcatta atatttaact gtatagactg
    16381 acgaaaaatc aatatcctga gcaactatta cattaataac tgttccaggt aatacatatc
    16441 ctgtaggtgg tatatttata gaattttcca gtgctttttc agccatactt tccgcattat
    16501 cctcactgtt atcaaaacta tacgttgatt tatttttttg tgccgcgttt gcagctgttg
    16561 ctatcgcgtc ctgaataaac gatagcatca cggctccacc aaacctctgc ccataatgat
    16621 tatcaatgta tgcatccgtg ccactgcttc ccatcggccc cgtccctaat ccatttaaat
    16681 ctgctcgtat acccccttct gtcccggcag tagtttcaag ctcactccat gaagtgaaca
    16741 ccgtagattg cccttgtttt acttcaattt tttgttcccc attcaattca gcacctgctt
    16801 cagccaaaat aacactaccg tccgctgaaa aaatgggttt tgttaaccga caaccaacga
    16861 atccagagtg atccgttttc accgccgtat aaagtacgca ctgaaaattt gttctccttt
    16921 tcaggagata cttacgtgga ggcgatttat aagctttagc tgttgcatac tggcgggatg
    16981 atgagagatc ccccaggatg ttagatctca tatccgaaga tattccccca ttcacagtat
    17041 ctgcctgact ctgtaaagca cggttaacta tctctgccgg atctgcattg gcaaactctc
    17101 gtagacgagc ctcttctgcc gaaacatcat aaccagaaga tgatgttaca ccgtcactac
    17161 taccaccaac tccttggcta atgccggaag aatcaaatcg tgcaacaggc gaaaacaacg
    17221 tttctggtac tctgatatcc gtaaccggtg taaccgggtt gaccgtagac tggccccctg
    17281 aagtttgagt attttttttg tctgaaacag gtccgttact ctcctgcttt cctgacgact
    17341 ggacggttct actgtcagat tccgttttgt ctctgtcttg tggtaaatga gcctccataa
    17401 acgc
//
LOCUS       DAZGTJ010000098        17383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000098 DAZGTJ010000000
VERSION     DAZGTJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.098"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1263
                     /locus_tag="ACHK8E_004103"
     CDS             <1..1263
                     /locus_tag="ACHK8E_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFP0346302.1"
                     /translation="KTSTSKEQEKEHEQAADTKNQTNVKESSEPHESPESRTVVKLEG
                     KLIDHGKAPLDNKKGAEQSYFVTVRGRDGKETTHWDSDLQNVMKGKRRGTEISLELKE
                     SNPVQVSTRDHLGNLSIREAVRQTWTLNKMSVGQEQKREQSLEKIQQNQSPIPARTQT
                     AVDLILANPVLRQMAMTAEQKEREFTALQADKESVREQTDQEIRNRLGSGDQSREPLA
                     VILSDDELRGMALSMEQKMREMATPVDKLDAVKAEINSEIAARVARNSSIPVPVSEVS
                     ATVAQKEIHREAESRGNKDKVHEGVLLDHGRAAYKFKPDISKPEDERDDSYFVKLKMD
                     NGKTRTLWGVGLESAVAGYSKGERLRLEDKGTEKVQWIERMKDGREIEKNGDRRVWDC
                     RSLTRESEVMKGFRVSGMPERDDGPDVA"
     gene            1667..1930
                     /gene="repA"
                     /locus_tag="ACHK8E_004104"
     CDS             1667..1930
                     /gene="repA"
                     /locus_tag="ACHK8E_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0346303.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            2153..2227
                     /gene="tap"
                     /locus_tag="ACHK8E_004105"
     CDS             2153..2227
                     /gene="tap"
                     /locus_tag="ACHK8E_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0346304.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCD"
     gene            2220..3077
                     /gene="repA"
                     /locus_tag="ACHK8E_004106"
     CDS             2220..3077
                     /gene="repA"
                     /locus_tag="ACHK8E_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0346305.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFDSLDI
                     SEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            4045..4317
                     /locus_tag="ACHK8E_004107"
     CDS             4045..4317
                     /locus_tag="ACHK8E_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP0346306.1"
                     /translation="METYIRTRVDDVLKSQFETILQDCGLSVSAALRLFAENVVRNEG
                     LPFEITRKPSARLRESIRQTEELMAQGRPGFENVGELIASMNNGEQ"
     gene            4304..4609
                     /locus_tag="ACHK8E_004108"
     CDS             4304..4609
                     /locus_tag="ACHK8E_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HFP0346307.1"
                     /translation="MGSNKRAPLPYRTDRTKNFEKAWKRYNKSGRYDMQAAAVAMNLV
                     TNRNPVPAEYRDHELTGEWAGFRELHIGGDFLLVYRVDEKNEIIYFTDLGTHAELFE"
     gene            5372..5830
                     /locus_tag="ACHK8E_004109"
     CDS             5372..5830
                     /locus_tag="ACHK8E_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346308.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSDDRWEAPQRAARLAAAV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            6258..6920
                     /locus_tag="ACHK8E_004110"
     CDS             6258..6920
                     /locus_tag="ACHK8E_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFP0346309.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            7019..7303
                     /locus_tag="ACHK8E_004111"
     CDS             7019..7303
                     /locus_tag="ACHK8E_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0346310.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LPAYFEDILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            7333..7560
                     /locus_tag="ACHK8E_004112"
     CDS             7333..7560
                     /locus_tag="ACHK8E_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346311.1"
                     /translation="MRKRDDYYDGLIAGGTVAIAVATLLTLLITVFVNNKENSEFHEW
                     INSHCEKENVENTSFKCDDGDGNIYIVKKEA"
     gene            8226..8681
                     /locus_tag="ACHK8E_004113"
     CDS             8226..8681
                     /locus_tag="ACHK8E_004113"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFP0346312.1"
                     /translation="MTTRKSGFNWYVLEFCSARTARVFSHLERLGVTFWCPLTCSHYR
                     RSDKNCFRRKEVPLFPGYVFIKIDFNKTHSTTVTSVPWVKSFVSFGGEPVPVCEEIIL
                     GLRRRETNYWGEYSPIRGIPHELAEILLENNPMKRSFLFFKYLSNTQNK"
     gene            8757..8936
                     /locus_tag="ACHK8E_004114"
     CDS             8757..8936
                     /locus_tag="ACHK8E_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346313.1"
                     /translation="MIYTKKECVWLGFLLIGVAFLGFMADDFTDNIYTFIWIMNSFFG
                     VFMLFMGVFISDDES"
     gene            9006..9251
                     /locus_tag="ACHK8E_004115"
     CDS             9006..9251
                     /locus_tag="ACHK8E_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346314.1"
                     /translation="MSTSNENLSNKKSPIYQIKQIYIKEDTKAQLMEFIKGINDECQL
                     LVKPTYIVHCLLNCALQSEGIRKEVINYVANAREKMR"
     gene            9251..9922
                     /locus_tag="ACHK8E_004116"
     CDS             9251..9922
                     /locus_tag="ACHK8E_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0346315.1"
                     /translation="MSILTTGAFLALAAQCAPNVHPDTSLDVVRVESGLHQYAIAEII
                     PKNERVAEGKSFISHYPESKDEAVKIIRRIEERKRNYSVGLMQINSSNFKKLRVTATE
                     LLEPCSNLKAYQYIITDCYLRGGSLTNALSCYYSGDFITGHKVESELNNTSYIRRIGL
                     LDNNYVVPGTREKINDIKDKTHDDIIFESWDILREYPKQLNGYGSKKEVLINERNNEK
                     DDRTV"
     gene            9885..10226
                     /locus_tag="ACHK8E_004117"
     CDS             9885..10226
                     /locus_tag="ACHK8E_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346316.1"
                     /translation="MKEIMKKMIGLFSIPSRYIALLFVFIPVYAHAAWYDPIVKFVKE
                     FQTGIIIVGGAVAVGSLVYVGVCWIISRMSGNMETTAMDYVKHIGVIGAVGGAVAAAT
                     WAYQLWGGTIS"
     gene            10226..10597
                     /locus_tag="ACHK8E_004118"
     CDS             10226..10597
                     /locus_tag="ACHK8E_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFP0346317.1"
                     /translation="MGETNEILEFDTNNAMNRVAMFAGIPVFALLFLVVIGCILTFSS
                     VWMWGWWGFIVTIPFILAIVGLRIICERDDKAFRRFRFQLRRWKMNRKYGRHLLLTSR
                     NPRWRDKYARRVIQKRILTGK"
     gene            10554..13088
                     /locus_tag="ACHK8E_004119"
     CDS             10554..13088
                     /locus_tag="ACHK8E_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFP0346318.1"
                     /translation="MQDALFKNAFSPESNAKVPWLGHHVAPEICTLGTSHILSLIRFS
                     GVPHDTRDQAMLNKEFMYLARCFKALGKQEGNNLKLQTYTFKRRIHLTHQYNLELPVL
                     QDLVDCWVKPFRNGTFRQVGYSMALILKFRDLDDGINRMRELLSLCREMLGDFGVSVM
                     ELEERDGKLYSQIGRFYSFILNGEEKDIPVTDTRLGDSIIDSETGFGAYDYVENRPSY
                     GKRRFASTYDLRDYPESKSYPGMWDEVLDEQYDFCMVQSFLFEDRNRMKRRLKTQMSD
                     LSSTEGQSSETEELADSVQEVTQGKKVFGEYYASLIVFGDTPEEAVDNGARMQSLLMA
                     RDTGFVRSRSTNYVTWLTLFPGYADVIYSMPKSTENLACGFSLHATPCGKADGNPPGD
                     GTALMPVMTENGGMYFLNAHASPLGKNCRGEKYPGHKTTIAMTGAGKTTVESLELVFF
                     SRWNPMIFSLDYNNSLENVLRALGTRYFNIKPGEFTWIQPFQMEDSVELRQYLFDVLV
                     QCAGGADSTEESKIQMSIDAVLKHKNVESRSFSLLLQSIPDTGPGGLRERLSKWCRDD
                     GNGRRGQYYWILDSPRNDFDPTLFRRLGFDCTKILNMEFATRHPQAMEVLLNTFFFLK
                     RRMHENEPGSLLINSIAEFWAPLMFESTANSIMEILHAGRTRGEMVMMDTQVPEYVLN
                     NERGPSVIQQTITQEWMANEKATSDSYSKFSVTSKEFSKIEEFSAYSRKVLVKQGGAS
                     VVLKVDMQDKLKYWLPLLSSDYANIPVAKRVREALGTDDPAVWVAPFLDEMVAIGVRK
                     ELAEKYPEQDVNDPQVWEPAFIEVMKQLERPVSMKLTIINNINSKS"
     gene            13158..13808
                     /locus_tag="ACHK8E_004120"
     CDS             13158..13808
                     /locus_tag="ACHK8E_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFP0346319.1"
                     /translation="MIRKSCIAACLSITLCSLSYAGGVPTVSIAELTQLVVNAQQQAQ
                     EALAQLNKAKEAIRQAKDQYDHYKSILQGNDKLGAFLNDPYINKLLPMKDWQDIYNQT
                     QDLTDLRKRYGITGYEPDVQKLFDKLLAQVDVYEKQYDATVTRVNHAEELRNKLNSVQ
                     TPKDREQLALRYQQEMLELENQRMQLENTRYLMEQKERIENKKNEEDFYNFMWGRS"
     gene            13834..14232
                     /locus_tag="ACHK8E_004121"
     CDS             13834..14232
                     /locus_tag="ACHK8E_004121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346320.1"
                     /translation="MKFILKLCFLMLLLSGCNDKAKDFIGGWTQESNEEYPVYITINY
                     DSGIYHVDVKKLDTYLVEKKKADAFDDFMWGKKSKNESENLTFDDCYKVFNFEAKAIS
                     SSVLEGKGISVRMDGDRLIFDGKRFVRKKS"
     gene            14258..15205
                     /locus_tag="ACHK8E_004122"
     CDS             14258..15205
                     /locus_tag="ACHK8E_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFP0346321.1"
                     /translation="MQVNVAETLFRTIDTATQSQMANMNTVMSVMGIVIGGIWMTYIL
                     MKSLYWYFEGLTTVIQDAFFTIFKAFAIMMASLTISWYTSTVIPPVLEFPLWLANTIA
                     GVNNDSSNLVDMVINSYISGLENLVDKMKFGFSVSSIKSMVTVGVAVVLYLLSGLPFL
                     GVAIATLLTLKVATNLILIIGPMFIACLAFPKVAHWFWGWVGVLGGFVLTQALFAITL
                     GMAISFINSNMINNGDFNISMVDIICMSLYFSAFTLLATEIPNYAASIMGGAPSGGVG
                     SMSRLLGKATGFGAASHMSKALGSKLVDRFHGRGKGRIS"
     gene            15422..16285
                     /locus_tag="ACHK8E_004123"
     CDS             15422..16285
                     /locus_tag="ACHK8E_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFP0346322.1"
                     /translation="MLFKSNKIKKETEQERLLHYGDGKLSGKSDEIGREIRAYTMAAR
                     WFEKRVAEDYRKKARNSRILAGLFGVIAIVAVIAVANLAPLKTVEPFVIRVDRNSGYM
                     DVVRPGWSKEDTKDISDDKHFISLYILSRETYNWASQKSNFAIVRQLSYSDVFSEYRN
                     FQLSGKGYVSTLNQSRQISVDIDSIVPLPVSTDKKLGERDDIKTYQVRFRQSLLDAEG
                     RPVPETGQILKLDENGKPIVKPKVVFWTAIISFDYLNTADTEGEAWLNPKGFGVLAYT
                     KTEEIRNEENN"
     gene            16269..17081
                     /locus_tag="ACHK8E_004124"
     CDS             16269..17081
                     /locus_tag="ACHK8E_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFP0346323.1"
                     /translation="MRKIIKYIVPVCLIFSVTGFVHAEIAGKGSVSDKRIQTALYSPD
                     NVFRIYAMKDRTTMIQLEQGETINTDAGAMNLGKPGTEGAPEWLLGANKAGSLIMIKP
                     SRYAEDPRTNLIVSTNRRTYLFELHLTQSPALATYLFRFNYPKPPEVGKNPFVGRNIN
                     ENPCDGIINKKYQKRGDMIISPYQVWDNGTFTCFRFPTNAPRPVIYEVLPDGTETMVN
                     ARPVNDILVVHGVSREFRIRLNRLVLAVRSKVENTGWYNYKGTTTGEVREVK"
     gene            17081..>17383
                     /locus_tag="ACHK8E_004125"
     CDS             17081..>17383
                     /locus_tag="ACHK8E_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HFP0346324.1"
                     /translation="MTENVVTPPENETKKETSAADENKPQGQGVGAFSLKPAGRKKPV
                     NWKIVGVIIGGIVIVLLFTIIFAAIVINNRADPEPEIDETGIKAEVVTRSIGVIDDP"
BASE COUNT         5240 a         3151 c         4115 g         4877 t
ORIGIN      
        1 aaaacgagta cgagtaagga acaagagaaa gagcatgaac aggcagcaga tactaaaaat
       61 caaacaaatg tgaaggagtc ctcagagcca cacgaatcac ctgaaagcag gacagtagtt
      121 aagctggagg gtaaattaat agatcacggc aaagcgcctc ttgataacaa gaagggtgct
      181 gaacaaagtt attttgttac ggtcaggggc agggacggta aagaaaccac acactgggat
      241 agtgatttgc aaaacgtaat gaaggggaaa cgccggggaa cagagatatc tcttgagttg
      301 aaagaatcta atccggttca ggtcagtaca cgcgatcatc tcggcaattt gtctatcagg
      361 gaagcggtcc ggcagacttg gacattaaac aagatgtcgg tgggacagga gcagaaaagg
      421 gaacagtcgc tggagaaaat acagcaaaat caaagcccca taccagccag aacgcagaca
      481 gctgttgacc ttattcttgc taatccagta ttacggcaga tggctatgac tgcggaacaa
      541 aaggaaagag aatttactgc attgcaggca gataaagaat ccgtcagaga acagactgat
      601 caggaaataa gaaatcgtct cggtagtgga gatcaaagca gggaaccgtt agccgtcatt
      661 ttgtcagatg atgaactacg tggaatggcg ttatcgatgg agcaaaaaat gcgggaaatg
      721 gctactccag tagataaatt ggatgcagtt aaagcagaaa ttaacagtga aattgcagcg
      781 agggttgcgc ggaacagcag tattcctgtg cctgtatcag aagttagcgc cactgtggca
      841 caaaaagaaa tccatcggga agcagagtcg agaggtaata aagataaagt acatgaaggt
      901 gtgctacttg atcatggccg ggcagcatac aaatttaagc ctgatataag caaaccagaa
      961 gatgaacgtg atgatagcta ttttgtgaaa ctgaagatgg ataacgggaa aacacgaaca
     1021 ctatggggag ttggtctgga gagtgctgta gcaggttata gtaaaggtga gcgtttgcga
     1081 ctggaggata aaggaacaga gaaagttcag tggattgaaa ggatgaagga tggaagagag
     1141 attgaaaaaa atggcgatcg cagggtttgg gactgtagat cgctgactcg tgaatctgaa
     1201 gtcatgaagg gattcagagt atcaggtatg ccggagcgtg atgatggtcc tgatgtagcg
     1261 taacttatac accgcgcctt atatccccgc ccttaagggc ggggttttaa aaccagcggg
     1321 tcgagcaagg caagttccga ccgttaacgt aatcccacat tcaagaactt acggtttcat
     1381 tctttgttca gcagttgaaa ccgtaagttt ctgttcaaat gccgtttcgt gagctgcccc
     1441 ctttttgatc cgggatttgt ggagggtgtt agttaagtaa tcacatgata tatcgacact
     1501 tgttccgttc ggagcaggat cttttctgaa tagcatcaga tctttatagc tgaactggcg
     1561 atctgtgcgc caacatggat ccgccagggt ataattaagt cgctgatttc aataaagtgt
     1621 agtatcctct gcaaaatgat ccttttttga tctatgagga ggctgtatgt ctgacgctgt
     1681 acgttcggca acttcctctg aagccagacc taaacgggca tatagaaaag gtgcaccgtt
     1741 aagcgttaat gagcgaaaga ggttgtctct caatcgaaag cgggatacac ataaagcgct
     1801 taatgttttc atccgtaatg aattgaaaga tattcttgag cgcttttgcc aggataagca
     1861 tttgaccttg ggtgaaggga tcgagctgct aatccagcag gagaaggaac gagctaaaaa
     1921 tggtgagtag tcacctttat ttgatccttt tctggcagag tgatagatta cggacagaaa
     1981 attcatggct ggccacgccg taaggtggca gggaactggt tctgatgtgg atttacagga
     2041 gccagaaaag caaaaacccc gataaccttt ctatgtttgg cgacgcagaa gattaccggg
     2101 gcccacttaa accgtatagc caacaattca gctatgcggg gagtatagtt atatgcccgg
     2161 aaaagttcaa tacttctttc tgtgctcact ccttctgcgc attgtaagtg caggatggtg
     2221 tgactgatct tcaacaaacg tattaccgcc aggtaaagaa cccgaatccg gtgttcacac
     2281 cccgtgaagg tgcaggaacg ctgaagttct gcgaaaaact gatggaaaag gcggtgggtt
     2341 tcacttcccg tttcgatttc gccgtgtatg tggcgtatgc ccgttcgcgc ggcctgcgcc
     2401 ggcgtatgcc gccggtgctg cgccgccggg cgattgatgc gctgctgcag gggctgtgtt
     2461 tccactatga cccgctggcc aaccgcgtgc agtgctccat caccacgctg gccattgagt
     2521 gcggactggc gacggagtct gaggccggga aactctccat cacccgtgcc acccgtgccc
     2581 tgaagttcct gtctgagctg gggctgataa cctaccagac ggaatacgac ccgacaattg
     2641 gctgtaacat cccgaccgac atcacgttca cgccggcact gtttgactca cttgatatct
     2701 cagaagaggc agtggcttcc gcacgccgca gccgcgtgga atgggaaaac aagctgcgca
     2761 aaaagcaggg gctggatgcg ctgggtatgg atgaactgat agcgagagcc tggcgttttg
     2821 tgcgcgagcg tttccgtagt tatcaggcag agcttaagtc ccacggcatg aaacgtgccc
     2881 gtgcgcgccg cgatgcaggc aggacccgtc aggacattgt caccctggtg aaacggcagc
     2941 tgacacgtga aatcgcggaa gggcgtttcc gcggaagcct ggaggccgtg aagcgcgaaa
     3001 tcgaccgtcg cgtgaaggaa cgcatgataa tgtcgcggaa caacaactac acacggctgg
     3061 ccacagcctc accctgagtt tgacctcatc tgaataatcc ggcctgcgcc ggaggcttcc
     3121 gcacgtctga agcccggcga cgcacaaaaa agcagcacag cacacaaaaa acagcctcat
     3181 catctgcctt ccggctcgcc ttgctccccc tgtttttcac gcaaaacacg tctcacagac
     3241 ggggaattct gtttatccac atttaactgc aagggatttc tccgtaaggt tacaaccgtt
     3301 catgctataa agcgccagcc gcaggcgtta cagggtgcaa tgtttctttt aaaccccagt
     3361 actgtatttc ctttaaacta cttaattact tccatttaaa aagaaaacct attcactgcc
     3421 tgtcctgtgg acagacatat atgcacctcc caccgcaagc ggcgggcccc gaccggagcc
     3481 actttagtta caacactcaa atacaagcac cgaaaaaaac taggtccaac gcagaactga
     3541 aaccacaacg cccctccctc ataactgaaa agcggccccg ccccggccca aagggccgga
     3601 acagagtcgc ttttaattat gaatgttgta actaaacatc atcatcgctg tcagtcttct
     3661 ggctggatgt tctcagtaca cgctcgtaag cggccctcac ggcccgctaa cgcggagata
     3721 cgccccgact tcgggtaaac ccttgtcggg accactccga ccgcgcacag aagctctgtc
     3781 atgactgaaa gcgggtatgg cttagcaggg ctggggatgg gtaaggtgaa atctgtcaat
     3841 cagttccggc ttacgccggg cttcggcggt tttactccgc tgccatatgc aacaacggag
     3901 taccgcctta catgcgctaa cgcgcataaa tccccgttcc tcagtggaac gaaaactcac
     3961 gctagctggg aaatatgcac cttgttttgt agtccattat ggactacatt agcaacacca
     4021 atatttgatt aatgaggtga tgagatggaa acatatatcc gtactcgtgt tgatgatgtt
     4081 cttaaaagcc aatttgaaac tatcctgcaa gattgcggat tatctgttag cgctgcattg
     4141 cggttatttg ctgaaaatgt tgtccgtaat gaagggttgc catttgaaat tacccgtaaa
     4201 ccatcagctc gtttgcgtga atccatccgt caaaccgaag aactgatggc acaagggcgg
     4261 ccaggttttg aaaatgttgg tgagctgatt gcgagtatga acaatgggga gcaataaacg
     4321 cgcaccgttg ccttaccgga cggatcgcac taagaacttt gaaaaggcat ggaaacgcta
     4381 taacaaaagc ggacgctatg atatgcaggc ggctgccgta gcaatgaacc ttgtgactaa
     4441 tcgtaatcct gttccggctg aatatcgaga ccatgaactt actggagagt gggctggttt
     4501 ccgagaattg catattgggg gcgattttct actggtttat cgcgtcgatg aaaaaaacga
     4561 gattatttat ttcactgatc ttggtactca tgctgaactc tttgaataaa aattgcgtca
     4621 cgcaatgact gccctccacg ctcacggtat caagaaatca gtcattgtcc tgtactgggg
     4681 gcagtttgga tattggaaat tattgtacgt taggagcatt ttttaatcga tactgataag
     4741 taactgattg atttatcgtg atcagctatc gcctttaagt tattgctttt ctcttatcgc
     4801 atttaggtta tctcatttct ctaaagatct gccaaagacc gtatagcgtt ggagtacacc
     4861 ctaccgcaat agcgcaaccg tacaaggggg ttcgcgccca tttcctctaa aagtctcacc
     4921 ttgcttaggg gtcagtttgg atatagagaa ttattgtacg ttcagcgcaa caaacttaat
     4981 gctaatgtat gcatacatta gcattaacca cacatttaag actggggttt gaggctcagt
     5041 ggaacaataa ccgccgttaa gcggtcattt taaatagcca aaaatcaacc agccaattta
     5101 catcgtgaat ctgcgttctt ggctatctgt tttggggggg ccaagttggc tttactcatc
     5161 ctggccgctt gcggaacaaa agacgttgtt gatctcaaca gattgattta cctgttattg
     5221 accattcaat tgaaccgctt aaccgccttt tgagttccac tactccccaa accccgtctt
     5281 cgccgcttgt tgctgtcaga tattaatgct aatgtatgca tacattagca ttaatgaggg
     5341 atgatatgtg agagacaaaa ctagggaggc catgcggctt tttttaggcg gcaggtgtta
     5401 cacggctgaa aaacttgaaa aggattatct ggctgaagtt gctaattaca gtgacgaccg
     5461 ctgggaagca ccgcaacgcg ccgcacgtct ggcagctgcc gtaaagcgtt acaagacatc
     5521 agaaatgctg cgctttattt ttgctaccat tgcatacgat cccgatccag atttaacccc
     5581 gctgactgtg agacgtttgt gcaaggcatt gttcggcaga accggaagcc agtggctggt
     5641 tgttgaagtt tttggggaga aggggcgtca gcaccgcagc gcggacagca atccggagat
     5701 ggtggaaaaa atggctgcca gatatcgtca tgccgccgaa ctacactggt ccgcaacgct
     5761 ggcagaaatt gaacgggtaa agcggcttta tcagacgaaa attaagaaat ccaaaaaaga
     5821 agtgggttga ctgccattgt tattcttttc cttcatcatt aatgctaatg tatgcataca
     5881 ttagcattaa tgatggtgcg ttgaacgtac aataatttcg aatatccaaa ctgacccctc
     5941 aaactcactc acactcaaaa agaaccagaa aacgtaccca taaccagaaa gtgacaatgt
     6001 catacacatg ttttacaaca taaagataat gtaaagacaa atgcagcttt accacgtaac
     6061 gcaaaaataa agacgcaaat acctttacaa agtaaaggta ttataaagat aatggtatct
     6121 ttatttgtgc tttacatggt aaagcttatg taatataaag acactgtaaa gacaaaatca
     6181 tctttacaaa aatcacacat tgtaaagaca atagagtctt tacacattct ttacatcata
     6241 aagagaggaa ttctgaaatg ggtaaaatct tgcttgtcgt atcagataag ggtggtgtag
     6301 gcaaaagcac ctacgtggct aatacaggct caatgctggt caacaaaggt aagtcggtaa
     6361 tcatcttgaa gacagataaa aaccacgatc tgttgagctg gaacgaaaag cggacagata
     6421 acggcttact cactatcccg gttcacgcag cttatgggaa cgtcagcaac gaaattaagc
     6481 gccttagtaa gctgtgtgaa gtgctcattg tcgattgccc cggccacgac agccaggagt
     6541 tccgtagcgc cctgacagta tcagatatcg tggtaacact agttaagccg tcctctgatt
     6601 tcgaaagcga aacactgacc agtgttacag agaaaatccg caccgctcag aaagctaacg
     6661 cagccctgga accgtgggtg ctgttcacaa gaatcaactc agctaaaccc cgccacagaa
     6721 aagcggccat tgatctggat aaattactgc gctcggacaa catctggatt caacctctga
     6781 aaacccggat atccgagctg gatgtgtatg aaagcgcctg taatgaaggg gcaggggtac
     6841 atgatgttag ccgggcatca agcctttcaa cagcgaaagc acagattgaa ctcgtggctc
     6901 aggaaattgg gattttgtag taatatcaat atgttaaatt attatcttta cattgtaaag
     6961 ataatgtaaa ggcggtatga tatttactat caatacatac cgtactaggg gaaatttcat
     7021 ggctttaaaa ctaaaccgtc ctaacatcga tgaatctcag caaccagcaa caaacgcaga
     7081 aaccgcgcgt tttatctccg gcgctaccag agcgcctgta gtgggtaaac caaagttgat
     7141 gaatttcaga ctgccagcgt actttgagga tatcctggaa gcggaggcgc agagaacagg
     7201 ccagagcaag acaaccgttc taaaggcagc gctggccgca ttcgataacc tggacgagaa
     7261 cctgaaaaat cattggctac ttgagtctgc aaagttaggt taacaagtaa ttcgcttatt
     7321 taggtttaaa aaatgagaaa gagagatgat tattatgacg ggttaattgc tggaggtact
     7381 gtggcaattg ctgttgcaac ccttctgaca ctgttaatta ctgtatttgt caacaataag
     7441 gaaaattccg agtttcatga atggataaac tcacactgtg aaaaagaaaa tgttgaaaac
     7501 acttcattta agtgtgatga cggggatggg aatatatata tcgtaaaaaa ggaagcgtaa
     7561 ttaagggcgg ggagggacca ttcctgttaa cgccctacag tcgtgacgaa tcagccagaa
     7621 gtgctggata tgtgtctgct gtttgccaga cgattttttc aagtaatacg cctgcaatcc
     7681 cgattagcgc aactacaacg cccgttctga taactgtttt cgctttcatt ttaacttaac
     7741 tgatcaagac gctgttaact ccatggtcgt acctgctgct ggggacagta catgtcagta
     7801 cttaccaaac aggcttcaaa aaagcaacag attcgctata tcggaagttt cgacggtttc
     7861 ggctctcctc cctgtatgac gactgcgtgt gcagtgctgg cttggttgtt caaaagttca
     7921 acgaaaacag tatgaaaaaa gaacatcaaa aaaaatattt atctggaaat tggtactaac
     7981 acgatctgtt attttgtttt tttatcaatc ggtagatcta cgatctattt gtgcggttgt
     8041 ttttagtgta aaattctgtg gaaatggatt aatatagatc taagatctac atattcgcat
     8101 atttacagat aaaagctttt atgtaatatg ttattacgct attaaaagtt gttttgattc
     8161 tgcgcaaaaa aacagggcta cggatacttc atgactgatc ggggatcccc gaggcggagg
     8221 tgtgcatgac gactaggaaa agcggcttca actggtatgt tctagagttt tgttctgcac
     8281 gtacagcacg agtgttttct catctggaaa ggttaggggt aacattctgg tgtcccctga
     8341 cttgttccca ttatcgtaga tcagataaaa attgtttcag acggaaagaa gtgccgctgt
     8401 ttcctggata tgtgttcata aagatcgatt tcaataagac ccatagcaca actgttacgt
     8461 cagttccttg ggtaaagtca tttgtgtcat ttggtggtga gcctgttcct gtttgcgagg
     8521 aaatcattct gggattgaga agaagagaga ctaattattg gggagaatat tctcctatac
     8581 gcggaatacc tcatgaattg gctgaaatct tgttagaaaa taatccgatg aaaagaagtt
     8641 ttttattttt taaatattta agcaatacgc agaataagta gttgaaaatc ttggtttatt
     8701 atcttttgtg tattcgcgga gagctataat aaaataataa attaaaagag gataaaatga
     8761 tatatacaaa gaaagaatgt gtttggcttg gttttctatt aattggtgtg gcgtttttgg
     8821 gttttatggc tgatgatttt acagataata tctatacatt tatttggatt atgaactctt
     8881 tttttggtgt ctttatgtta tttatggggg tgtttatatc tgatgatgag tcatgaatca
     8941 ataaatcata ggttatttga aatgttatgt tttaatatgc accaagtgat ttttcaggag
     9001 agtatatgag tacatctaac gaaaacttat caaataaaaa atcgccgata tatcagataa
     9061 aacagatata tattaaagaa gatacaaaag cgcagttaat ggaatttatt aaaggaataa
     9121 atgatgagtg tcaattactt gtaaagccaa catatattgt tcattgtctt ttaaactgcg
     9181 cattacagag cgaaggaatc aggaaagaag ttattaatta tgttgctaat gcacgagaaa
     9241 aaatgcgctg atgagtatac tgactaccgg ggcatttctc gctctggctg cacagtgtgc
     9301 cccaaacgtt catccagata catctcttga tgtggttcgt gttgagtcgg ggttacatca
     9361 atatgctata gcggaaatta tacctaaaaa tgaaagggtt gcagagggta aaagctttat
     9421 aagtcattac ccggagagta aagatgaagc agtgaaaata atcaggagga ttgaggaacg
     9481 gaaacgaaat tattctgttg gattaatgca gattaacagt tcaaatttca aaaaacttag
     9541 agtgacagct actgaattgt tggagccgtg ctctaatctg aaagcgtatc aatacattat
     9601 taccgattgt tatttgcgag gaggtagcct tactaacgcg ttaagttgtt attattctgg
     9661 agattttatt acaggccaca aggttgaatc agaattaaat aatacaagtt atattcgaag
     9721 gataggatta ttagataata attatgttgt tcctggaacg agggaaaaga ttaatgacat
     9781 aaaagataaa actcatgatg atattatttt tgaatcatgg gatattctca gggaatatcc
     9841 aaaacaatta aatggatatg gatcaaaaaa agaggtttta attaatgaaa gaaataatga
     9901 aaaagatgat aggactgttt agtataccat ctcgttatat tgcgttatta tttgttttta
     9961 ttcctgtata tgctcatgca gcatggtatg atccaatagt taaatttgtt aaggagtttc
    10021 agacaggaat tattattgtc gggggcgctg ttgctgttgg ttctcttgtc tacgtgggag
    10081 tctgttggat tatttctcgt atgtccggga atatggaaac aacggcaatg gattatgtga
    10141 aacatattgg tgttattggt gctgttggtg gcgctgttgc cgcagctacc tgggcgtacc
    10201 agttgtgggg aggaacaatt agctgatggg agagacaaac gaaattctcg agtttgacac
    10261 taataacgcg atgaacaggg tggctatgtt tgcagggatc cctgtttttg cacttctttt
    10321 tttggttgtt attggctgta ttttgacatt ttcttctgta tggatgtggg gatggtgggg
    10381 atttattgtc accatccctt ttattctggc aatcgtagga ttgcgtatta tttgtgaacg
    10441 tgatgacaaa gcgtttcgtc gttttcgttt tcaattacgc cgctggaaaa tgaatcgtaa
    10501 atatggtcgt cacttactgt tgacttccag aaatccccga tggagagata aatatgcaag
    10561 acgcgttatt caaaaacgca ttctcaccgg aaagtaatgc taaggtgccc tggttggggc
    10621 accatgttgc acctgaaatt tgtactctgg gcacgtctca tattctcagt ttgatccgtt
    10681 tttctggtgt tcctcatgat acacgagatc aggctatgct aaataaagag tttatgtatc
    10741 ttgccagatg ttttaaagct ttgggaaaac aggaagggaa taacctgaaa ttgcagacgt
    10801 acacttttaa gcgtcgtata catctcacac atcagtataa tcttgaatta cctgttcttc
    10861 aggatttggt tgattgctgg gtaaaaccat ttcgtaatgg gacattccgg caagttggat
    10921 attctatggc ccttatcctt aagtttcggg atctggacga tggtattaac cgaatgcggg
    10981 aattgttatc tttatgtaga gagatgctgg gggattttgg tgtttctgtt atggagctgg
    11041 aggagcggga cggaaaactg tattcgcaga ttggccgctt ttactcgttt attctgaacg
    11101 gtgaagagaa agatattcct gttactgaca cccgcctggg agactcgatt attgacagtg
    11161 aaacaggatt tggcgcttac gattatgtgg aaaaccgccc gtcgtatgga aagcgccgat
    11221 ttgcttctac ttatgatcta agggattatc cagaaagcaa atcctaccca ggaatgtggg
    11281 atgaagtgct ggatgaacag tatgactttt gtatggttca atcttttctt tttgaagatc
    11341 gtaaccgaat gaaacgtcgc ctgaaaactc agatgtcgga tttatcgtct acggaaggac
    11401 aatccagtga aactgaagaa cttgctgata gtgttcagga agttacccag gggaaaaaag
    11461 tttttggtga atattatgcg agcctgattg tatttggaga tacacctgaa gaagctgttg
    11521 ataacggagc aagaatgcaa tcgttgttaa tggcgcgcga taccggcttt gttcgttccc
    11581 gaagtacaaa ttatgtaacc tggctgacgc tatttcctgg ttatgcggat gtgatttatt
    11641 cgatgccaaa atctacagaa aacttggcct gtggattttc cctacacgcg actccgtgtg
    11701 ggaaagccga tgggaatcct cctggagacg gaacagcgtt gatgccagtg atgacggaaa
    11761 acgggggaat gtattttctg aatgcacacg ccagtccttt agggaagaac tgtagaggtg
    11821 agaagtatcc agggcataaa acgacaattg ctatgactgg ggcaggtaaa acaacggttg
    11881 agagccttga attagtgttt tttagtcgct ggaatcctat gatattttct ctggattaca
    11941 ataattcgct ggagaacgta ctccgtgctc ttggaacccg ttattttaat ataaaacctg
    12001 gtgaatttac ctggatacaa ccgtttcaaa tggaagattc ggttgaacta cgtcaatatc
    12061 tgtttgatgt tcttgttcag tgtgccggag gtgctgacag cactgaagaa tctaaaatac
    12121 agatgtcgat tgatgctgtt ctgaagcata aaaatgtaga aagtcgtagt ttttcactgt
    12181 tattacagtc tatacctgat accggtcctg gcggactcag ggaacgatta tcaaagtggt
    12241 gtcgtgatga tgggaatgga agacgtggac agtattactg gatcttggac agtcccagaa
    12301 atgatttcga ccctacgttg tttcgccgtc ttggatttga ttgtacaaaa attcttaaca
    12361 tggagtttgc tacacgtcat cctcaggcaa tggaagtatt attaaatacc ttctttttcc
    12421 tgaagagaag aatgcatgag aatgaaccag gcagcttact aataaacagt atagcggaat
    12481 tttgggcacc tctaatgttt gagagtacag ctaattccat tatggaaatt ttacatgcag
    12541 ggaggacccg tggtgagatg gttatgatgg atactcaggt tcctgaatat gttctgaata
    12601 acgagcgtgg tccttcagtt attcagcaga caattacgca ggaatggatg gcaaatgaaa
    12661 aagctacctc agacagttac agtaaatttt ctgtaacttc taaggagttc agtaaaatag
    12721 aggagttttc ggcttattca cgtaaggttc tggtaaaaca gggaggtgca tctgtcgttc
    12781 tgaaggtgga tatgcaggat aagctgaaat attggttgcc tttattgtca tctgactatg
    12841 ccaatatacc tgtagctaaa cgagttaggg aagcgttagg tacagatgat ccggctgtat
    12901 gggtggcccc atttctggat gagatggtgg ctattggagt aaggaaagag cttgcggaaa
    12961 agtatcctga acaagatgtc aatgatcctc aagtatggga gcctgcattt attgaagtga
    13021 tgaagcaact ggaacgtcca gtttctatga agttgacaat catcaataat ataaactcaa
    13081 agtcttgatt ttatgatatc aggaatgctt tgatgtttaa cgaaaacgtt cattcctgat
    13141 aaacacagga gttctatatg atacgtaaat catgtatagc cgcgtgtctc tctataacgt
    13201 tatgttctct aagttatgca ggtggagtgc caacggtaag cattgcagag ttaacgcaat
    13261 tagttgtaaa tgcacagcaa caggcgcagg aagcgttggc gcagcttaat aaagcaaagg
    13321 aggcaattcg acaggcaaaa gatcagtacg atcattataa atcgattctt cagggaaacg
    13381 ataaattggg ggcatttctg aatgatccat atataaataa attactgcca atgaaagatt
    13441 ggcaggatat atataatcaa acccaagact taactgattt aagaaagcgt tatggtatta
    13501 ccgggtatga acctgatgtt caaaaattgt ttgataagtt acttgctcaa gttgatgtat
    13561 atgaaaaaca atatgatgca acagtcacac gagtaaacca tgctgaagag ttgagaaata
    13621 aactaaattc agttcagact ccaaaggata gggagcagtt agcattaaga tatcaacagg
    13681 aaatgttaga gcttgaaaat cagagaatgc aactggaaaa tacgcgctat cttatggagc
    13741 aaaaagaacg aattgaaaat aaaaagaatg aggaggattt ttataatttt atgtggggtc
    13801 gctcataatc atattatata acgggaggaa tatatgaaat ttattttgaa attgtgcttt
    13861 ttaatgttgc ttttgtctgg atgtaatgat aaagcaaaag actttatagg agggtggact
    13921 caggaatcaa atgaagaata tccagtatat ataaccataa actatgatag cggaatttat
    13981 catgtggatg ttaaaaaact agatacttat ctggtagaaa agaaaaaagc tgatgcattt
    14041 gatgatttca tgtgggggaa aaaaagtaag aatgaatctg aaaatttaac atttgatgat
    14101 tgttataaag tgttcaattt tgaagctaaa gcaataagta gttcggtatt agaaggtaag
    14161 ggtatatctg taaggatgga tggtgataga ttaatttttg atggaaaaag atttgtaagg
    14221 aaaaaatcat aatgtgacat agctaaagga tttttatatg caggtaaatg ttgcggaaac
    14281 gctgtttaga acaattgata ccgcgacaca atctcagatg gcaaatatga atactgtcat
    14341 gtctgttatg gggattgtga taggcggtat atggatgaca tatatattaa tgaagtcatt
    14401 atattggtat tttgagggct taacaactgt aatacaggac gcatttttta caatatttaa
    14461 agcatttgca atcatgatgg catcattgac tatatcatgg tatacatcaa cagttattcc
    14521 tccagtttta gaattccctt tatggttggc taatacaatt gcaggagtta acaatgattc
    14581 gtcaaattta gttgatatgg taataaattc atatatatca ggactggaaa atctggttga
    14641 taaaatgaaa tttggatttt ctgtatcatc aataaaaagt atggttactg tcggtgttgc
    14701 tgttgtttta tatttactaa gtggattgcc ttttttaggg gtggctatag caacattgct
    14761 gacgcttaaa gtcgcaacaa atctaattct tatcattggt ccaatgttta ttgcatgttt
    14821 agcttttcct aaagtagctc attggttttg gggatgggtg ggagttttag gaggatttgt
    14881 tcttacacag gccctttttg ctattactct tggaatggct atttcattta ttaatagcaa
    14941 tatgattaat aatggggact ttaatatatc aatggttgat attatatgta tgtctttata
    15001 ttttagtgca tttactttac tggctacaga aattccaaat tatgctgcat ctattatggg
    15061 aggcgctcca tctggtgggg tagggagtat gtccaggctt ttaggaaaag caactggttt
    15121 tggtgctgca agtcacatga gtaaggcatt aggaagtaaa ttagttgatc gttttcatgg
    15181 tagagggaaa ggtcgtattt cctagtaatt tttctatctc tgcttataat ccattttctc
    15241 gtcttcttta caaaaacttc atttataaaa aggcttatca tgaaaaatat attcgctata
    15301 tgtgcggcgc tttgtgttgt gctgggatgc atatcatgta gttcaccgcc agagttgtcc
    15361 caaccatctg gtgaatggat tgatgtaaat ccagagtctg tgctgttacc gggagaaaaa
    15421 aatgttattt aaaagtaata agataaaaaa ggaaactgaa caggaacgtc tcttgcatta
    15481 tggtgacggc aaattatcag gtaagagtga tgaaattggg agggaaatac gggcttacac
    15541 aatggctgcc cgttggtttg aaaaaagggt ggctgaagat tacagaaaga aagctagaaa
    15601 tagtcgtatt ttggctggat tgtttggtgt tatagctatt gttgctgtaa ttgcagttgc
    15661 aaatttagct ccacttaaaa cagtagagcc ttttgttatc cgagtagatc gcaatagtgg
    15721 gtatatggat gttgttcgtc ccggatggag taaggaagat acaaaagata tttctgatga
    15781 taagcatttt atatcattat atattttatc tagagaaacg tataactggg ctagtcaaaa
    15841 atctaatttt gcaatagtaa ggcagttgtc ttattccgat gtgttttctg aatatcgtaa
    15901 ttttcagttg tcaggtaaag ggtacgtctc aacattaaat cagtctcgtc agatttctgt
    15961 tgatattgat tcaatagttc ctttacctgt cagtactgat aaaaaattag gtgagaggga
    16021 tgacatcaaa acttatcagg ttcgtttcag acaatcactg cttgatgcag aagggcggcc
    16081 tgttccagag acaggacaga tactgaagtt ggatgaaaac gggaaaccaa tagttaagcc
    16141 caaagtggtt ttctggacag caataatatc ttttgattat ttaaatacgg ctgatacgga
    16201 aggtgaagcc tggttaaatc ctaagggctt tggtgtgctt gcatatacaa aaacagagga
    16261 gatcaggaat gaggaaaata attaaatata tagttcctgt atgtcttata ttttcagtta
    16321 ctggttttgt tcatgcggaa atagccggaa aaggttcagt cagcgacaaa aggatccaga
    16381 cggcattgta tagtcctgat aacgttttca gaatttacgc catgaaggac agaacaacaa
    16441 tgatacaact ggaacaaggg gagacaataa atactgatgc gggtgcaatg aatctgggga
    16501 aaccaggaac agagggggca cctgaatggt tactcggtgc taataaggcc ggaagtttaa
    16561 taatgataaa accaagtcga tatgcggagg atcccagaac aaacttaatt gtaagtacga
    16621 atcgccggac atatttattt gaattacatc tgacacaatc accggcttta gcaacatatc
    16681 tgttcaggtt caattatcca aaaccgccag aagttggtaa aaatccattt gttgggagga
    16741 atataaacga aaacccgtgt gatggtatta ttaataaaaa ataccaaaaa cgtggtgata
    16801 tgattatttc gccttatcag gtatgggata atggtacatt tacctgtttt cgttttccga
    16861 caaatgcgcc acgtccagtt atttatgaag ttctacctga tggtacagaa actatggtta
    16921 atgcgcgtcc tgtaaacgat attttggttg tgcatggtgt tagtcgtgag ttcagaattc
    16981 gtcttaacag actggttctt gctgtacgtt ctaaagtaga aaatacagga tggtacaatt
    17041 ataagggaac aactacaggt gaagtcaggg aggtcaaata atgactgaaa atgtagtgac
    17101 accaccagag aatgaaacga aaaaggaaac tagcgctgct gatgaaaata agcctcaagg
    17161 tcaaggtgta ggcgcatttt ctttaaaacc agctggtcgt aagaagcctg ttaactggaa
    17221 aatagttggt gtaattattg gcggtattgt tattgttctt ttgtttacaa ttatatttgc
    17281 cgcaatagtt attaataatc gtgcagatcc agaaccagaa atagacgaaa cagggattaa
    17341 agcggaggtc gtaactcgct ctataggggt aattgatgat cca
//
LOCUS       DAZGTJ010000099        17357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000099 DAZGTJ010000000
VERSION     DAZGTJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.099"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(101..832)
                     /gene="rlmB"
                     /locus_tag="ACHK8E_004126"
     CDS             complement(101..832)
                     /gene="rlmB"
                     /locus_tag="ACHK8E_004126"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP0346325.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(923..3364)
                     /gene="rnr"
                     /locus_tag="ACHK8E_004127"
     CDS             complement(923..3364)
                     /gene="rnr"
                     /locus_tag="ACHK8E_004127"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP0346326.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3403..3828)
                     /gene="nsrR"
                     /locus_tag="ACHK8E_004128"
     CDS             complement(3403..3828)
                     /gene="nsrR"
                     /locus_tag="ACHK8E_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP0346327.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4033..5331)
                     /gene="purA"
                     /locus_tag="ACHK8E_004129"
     CDS             complement(4033..5331)
                     /gene="purA"
                     /locus_tag="ACHK8E_004129"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP0346328.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5435..5632)
                     /gene="yjeT"
                     /locus_tag="ACHK8E_004130"
     CDS             complement(5435..5632)
                     /gene="yjeT"
                     /locus_tag="ACHK8E_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP0346329.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5714..6718)
                     /gene="hflC"
                     /locus_tag="ACHK8E_004131"
     CDS             complement(5714..6718)
                     /gene="hflC"
                     /locus_tag="ACHK8E_004131"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP0346330.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6721..7980)
                     /gene="hflK"
                     /locus_tag="ACHK8E_004132"
     CDS             complement(6721..7980)
                     /gene="hflK"
                     /locus_tag="ACHK8E_004132"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP0346331.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8066..9346)
                     /gene="hflX"
                     /locus_tag="ACHK8E_004133"
     CDS             complement(8066..9346)
                     /gene="hflX"
                     /locus_tag="ACHK8E_004133"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP0346332.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9423..9731)
                     /gene="hfq"
                     /locus_tag="ACHK8E_004134"
     CDS             complement(9423..9731)
                     /gene="hfq"
                     /locus_tag="ACHK8E_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP0346333.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9817..10767)
                     /gene="miaA"
                     /locus_tag="ACHK8E_004135"
     CDS             complement(9817..10767)
                     /gene="miaA"
                     /locus_tag="ACHK8E_004135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP0346334.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10760..12607)
                     /gene="mutL"
                     /locus_tag="ACHK8E_004136"
     CDS             complement(10760..12607)
                     /gene="mutL"
                     /locus_tag="ACHK8E_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP0346335.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12617..13954)
                     /gene="amiB"
                     /locus_tag="ACHK8E_004137"
     CDS             complement(12617..13954)
                     /gene="amiB"
                     /locus_tag="ACHK8E_004137"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP0346336.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13973..14434)
                     /gene="tsaE"
                     /locus_tag="ACHK8E_004138"
     CDS             complement(13973..14434)
                     /gene="tsaE"
                     /locus_tag="ACHK8E_004138"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP0346337.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14406..15953)
                     /gene="nnr"
                     /locus_tag="ACHK8E_004139"
     CDS             complement(14406..15953)
                     /gene="nnr"
                     /locus_tag="ACHK8E_004139"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP0346338.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15952..17091
                     /gene="queG"
                     /locus_tag="ACHK8E_004140"
     CDS             15952..17091
                     /gene="queG"
                     /locus_tag="ACHK8E_004140"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP0346339.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17074..17127)
                     /locus_tag="ACHK8E_004141"
     CDS             complement(17074..17127)
                     /locus_tag="ACHK8E_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346340.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3944 a         4949 c         4350 g         4114 t
ORIGIN      
        1 aatattaata aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa
       61 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
      121 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      181 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      241 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      301 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      361 ccagattagt cacccgaatc agcggaacgc tttctgccgc accacaggcc acttttttcg
      421 ccgtggcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acaccagcgg
      481 cgtccgcgct acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      541 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgcc
      601 gtcctggctt cacacgagcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      661 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      721 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      781 cgcgctccag cagggcctgc actgcatgga tgccgtaaat catttcgctc attaatgtac
      841 tcgttattta cgttttgccg ggagcgccac gcttgcccgg cctacggata attcaccctc
      901 ttctgtttga agagggtaat gatcactctg ccactttttt cttcgccgca cgcttcgctt
      961 tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt
     1021 tcttcgctgc tttcggcttc ggttttttct cgccgcggaa ggcgctgtct ggctcaaagt
     1081 tgaccttttt gccgacctga cgccgcttgc cgcctttttt gcctgcatcg ccttttttcg
     1141 ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa
     1201 agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca
     1261 ggcgataagt ctggccgctg gattcgccca tcaggcgttg ccccacctgg tcaaagcgat
     1321 agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcatccaggc
     1381 gaacaaagaa gccaaaacca gtgacgctgg aaattacgcc tttaaagacg ttacctacct
     1441 ggtcgagcat gaagtcacac ttcagccagt cagccacatc gcgcgttgct tcgtcggcac
     1501 gacgttccgc catcgaacag tgctgaccca gttgcagcat ctcttccatc gaataatggt
     1561 agccgccggt ttcagtggta ttcccctgat gcccctgctc tttcgccaac agatacttaa
     1621 tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg
     1681 actgcaatgc cagaccgaag tgaccacggt tttccggatc gtaaatcgct tgtttcatcg
     1741 agcgcagcag catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca
     1801 gctctgcgta gtcgcgcggt tccggcttgt taccgcccgg cagttccagc cccagctccg
     1861 ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca
     1921 gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc
     1981 actcttcaat taacttgtgt gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc
     2041 gttcggcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt
     2101 cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg
     2161 gggcatactg ctcgcgcaga tcctgatcgc cttgcagtat atgccagact ttggtgtagg
     2221 tcagacgcgc gtgagaactc atcaccgctt cgtagaattt gtagccagtc aggcggcctt
     2281 tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgagc
     2341 acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg
     2401 acgtaccacg gttacgcgct tctctgtcca gcggtgttgg cggacgcaca tagtaactga
     2461 cgtcggcaat tgcgacccat aaacgccagc cgccgccgcg ttttttctca cagtaaactg
     2521 catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcagat
     2581 cgacacggcc cgcttttgct tcttccggca cttcttcttt cagcccggca acctgttgct
     2641 caacagcctg cggccagatg tacggaattt catgggtacg cagagcaata tcaaccgcca
     2701 tgccggtgcc catattatcg cccagcactt cgacgatttt acccaccgct ttggtacggc
     2761 gagtcgggcg ttgagtcagt tcaaccacca ccacaaagcc catccgcgcg cccatgatct
     2821 gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga
     2881 cgcccgcttc ggtgaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga
     2941 caatacgcgc ttcacgacga cctttacggt cagcgcccag cggctgagcc agcacctgat
     3001 cgccatgaat gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt
     3061 caactcgcag aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga
     3121 ggcgttccgg cagcgcatag cactgacggc gagtgaagac cagttgacca tcgcgctcca
     3181 tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta
     3241 ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt
     3301 cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg
     3361 acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa
     3421 tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt
     3481 aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg
     3541 gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc
     3601 aatacgtatt gcactcgccg gtttacccaa gcgaatgccg ccattttttc cacgaacagc
     3661 agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat tacgggagac
     3721 gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat
     3781 gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa
     3841 agggaaatcg gggaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct
     3901 aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta
     3961 atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca
     4021 ggcgtaccag aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg
     4081 accagtagag atgatatcga tcggcacacc ggtcagctct tcaatgcgct tgatgtagtt
     4141 cagtgccgcc tgcggcaggc cgctacgatc tttcacgccg aaggtggatt cagaccagcc
     4201 cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt
     4261 agtcacttca cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc
     4321 atccagaacg tccagtttag tcaggcagaa gccagacaga gagttcagct gtaccgcacg
     4381 acgaacggca acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcgccgaa
     4441 ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg tcgggaacgg
     4501 acctgcaccc acacgagtgg agtaagcttt gaggataccc agaacgtaat caacataacg
     4561 cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta
     4621 cggatatgta ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa acatgacgaa
     4681 atcgccacgc tgacgcgcct ggtcaagcag gtcagaaacg tcaacaacca tagaagtcag
     4741 gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt
     4801 gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa
     4861 ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata
     4921 agcaggcccg atacctcgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg
     4981 cgcgttatcc agcgcaacgt gataatcaag gatcagcgga catgcttcgg acagcagcag
     5041 acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcagc
     5101 cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga
     5161 tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg
     5221 accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc
     5281 tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa
     5341 tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc
     5401 actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt
     5461 agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg
     5521 gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta
     5581 aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc
     5641 cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac
     5701 cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc
     5761 tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca
     5821 ggctacggat aaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg
     5881 ctgcttctgc atcgccttca ccacgcatga tgcggccctg acgctcagct tcagccagcg
     5941 ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg
     6001 aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag
     6061 acacttcggt cggcaggttg atctgcttaa tacgcacatc gacaacttca atacccagcg
     6121 ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct
     6181 ctgccgcttc ggcaatggcg ttatctgctg ccggggtagt aacttcatct tctgtacccg
     6241 cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga
     6301 cgatgtcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt
     6361 tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag taacggctga
     6421 aatcgctgat gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca
     6481 caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt
     6541 caatgaacgg tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt
     6601 cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga
     6661 cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg
     6721 ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat
     6781 atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg
     6841 cggcagacgc agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc
     6901 tttcagcatc tggtctaacg gcagaaccat caagttgcca cctttatcgt taaccagcac
     6961 tttgcgggtg ttgcccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat
     7021 ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc
     7081 ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc
     7141 gttcgcacgc ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc
     7201 gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc
     7261 tgcctggaag ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc
     7321 cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat
     7381 ggtgtattta ccgataaccc cacgcagggc gctgtcggta gcctgacgca ggctgtcatc
     7441 cgggctggtc acgctataca gatatttttc cggatcggtg acgcggtact gcacgttcat
     7501 ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg
     7561 cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc
     7621 cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccacgtt cggcttcttt
     7681 aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac
     7741 gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt
     7801 gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa
     7861 atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg
     7921 tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat
     7981 gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg
     8041 actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt
     8101 tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct
     8161 cctccatcca ctctttttct attgcctgaa gctgataaaa acgacttctc agacgccctt
     8221 cctgcggtgg cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag
     8281 cctgaaaaag ctgtggtatc ccctctccgg tctgtgcgga aagccagaca cggatcggtt
     8341 tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca
     8401 tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt
     8461 cgatgttttc ttgtacacgc acatccgccg catcaatgac gtgcagcagc aatgtggctt
     8521 gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa
     8581 taaaccctac ggtatctgcc aacacggttt caccgacatc tgcgacgtca atacgccgca
     8641 acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc
     8701 gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt
     8761 cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg
     8821 actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac
     8881 ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca
     8941 ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac
     9001 gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc
     9061 acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg
     9121 aagcacccgt cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat
     9181 actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg
     9241 aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga
     9301 tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct
     9361 aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa
     9421 ccttattcgg tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga
     9481 tggtagttac tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagac
     9541 gggacaacag tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg
     9601 atcacgaact gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa
     9661 atagaaactg gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc
     9721 cccttagcca ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg
     9781 aaaaaattgc gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac
     9841 ctgtaatact tcgtcacgcg cttgttctgg tttttcactg tcaagccagt gaaccccttc
     9901 ccaaccacgc agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg
     9961 ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc
    10021 cacgcaacga atggaaggca aatccgtatg caaatctcct cgggcaaaaa gcgcccggac
    10081 ttctgcttca aaacctgaag ccaacatctg atgaaaacgc tgctcaattc gttgatggag
    10141 cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc
    10201 tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg
    10261 ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg
    10321 aagttgacga tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg
    10381 cacttccggg tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa
    10441 catcgtaccg cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc
    10501 cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag
    10561 caatcggtgc ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat
    10621 ccctttgtaa ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag
    10681 ctcaatggct aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg
    10741 caggctcgcc ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa
    10801 cagattgtaa cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt
    10861 ccgccagcag ggttatggcc tgcgccattg accactgcgc atgttcgctc atcagatttc
    10921 gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta
    10981 tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga
    11041 tggtcacatg ctgcgcatcg gactggaaat caatacccaa ttccgccagg gcagactgcg
    11101 ctttttctaa tgcagatttt tcttcgccag aaacttttag ccgcagcgga atcagcaacg
    11161 gctgggcgca aacgggcgct tcacccggcg tcaattgtgc ctgacgcagc caacgttctg
    11221 ccactggcaa ggctaaaagt gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat
    11281 ggacgatagt cagtacccga ccaaagcttt gactgttcgc cgcaagtgca ggttcctgcg
    11341 gttctggcgc ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt
    11401 caccttgctg tttctggtaa cctggctgcg cattcggcca gggggcagcc ggacgactgc
    11461 ctgaggcggg cgcaggagtg taaagcggag ctactggctc acgaactgcc ggttcagcaa
    11521 aatgattgcg ccccgccgcc acgcggtttt ccgggatggc gcgcggtgca ggttgtggtt
    11581 catcgtccag cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat
    11641 agataaagtc atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta
    11701 cgttgacgtc cacctgatgc gggtcgatct ccagatacag cacaaacgcc ggttgttgat
    11761 cggcccccag tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca
    11821 tcatgcgacc attcacgtag caatactgaa tttccgccag tgcgggcgtg gtgtgattcg
    11881 gatctgccac ccagccgcgc agcgtaagat cgccgtgttg ccattcaatc gccagcgctt
    11941 gttcgagaaa agcggtgccg caaatcgccc ctaaacggcg ctctttttgt ccaccttccg
    12001 gcacggcgcg gtactggcgg accattttcc cgttatgcga caggttgatc gtgacgtcga
    12061 aacgcgccag cgcaatgcgg cggatgatct catcaatgtg gttaaattcg gttttctcgg
    12121 tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtag
    12181 tccccacagg atgcgccgcc ggttttaccg tcacgtccat atcgcgccct tcggcatagg
    12241 cctgccaggc ttcctgctgt tctacggtgc gtgaagtgag cgtcaggcgg gaaaccgaac
    12301 tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca
    12361 gagaggcgat tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga
    12421 taccgcagcc gttatcacga atgcggataa gtttcgcccc gccacgttcg atatcaatat
    12481 caatgcgcgt cgcacctgca tcgaggctgt tttccaccag ttctttgact accgacgcag
    12541 gtcgctcgac cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgta
    12601 ttggcatcaa tcgtccttag tttggcagcg tacgatctgg cgtcgtcacc gtactggcag
    12661 tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca
    12721 gacctttata aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt
    12781 cttcgctgtt gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgaac
    12841 gcagaacgcc aaggctggcg tgttctggtc gacgtttatg tatttcgcca atgcgttgca
    12901 actggctgat catactggtc gctacatcat accctacacg ctgggaatga ccgaactgta
    12961 aatccagcac cgcctggctt aaatagggat cagactgact gttcgccagc acatcgcccg
    13021 ctccgcccag cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcgctgt
    13081 tggcgcgacg gttagagagc acccatacgg aagcgccagt cgcactgcgg tttggtgcgg
    13141 catcagcgtg aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcgctgcgcc
    13201 ccatcaccga gataaagtaa tccccgtcac gggttaaaac gcctttaaac attggatcgt
    13261 cattgagaaa agtacgcaat ttacgtgcga tggcgatggt gacatttttc tcccgcgtac
    13321 caccggggcc gatagcgcca gggtcctgac cgccgtgtcc tgcatcaata gcgatgataa
    13381 ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga
    13441 taacacctgt agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc
    13501 gcggtgcgac aaccgcaggc gtttcaacgc gtttagcaac cacaggcggc gaaggaggcg
    13561 gtggcggcac atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct
    13621 tcaccgcttc ggttttaccg ttttcggtta gatcgaccac cagccgtagc gtttgtgcat
    13681 ctttaggcgt tccggagcga atcgccttca ccagattatt gccgctaaac aacaacggca
    13741 gtccctgaat cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc
    13801 taaacgcata atcaggatcg ccaataaaac tcaacgttat ccgcgcctgc tgattgccgt
    13861 tagaaacctg aatatcagag agcgtcgcgg cacccaccgg cgcgcacagc agcagcaacg
    13921 tcgctaccaa ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg
    13981 ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc
    14041 cttgtgcctg ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt
    14101 gttgtggcca ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa
    14161 actccagctc ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc attaaattgt
    14221 cgagcgtata gggttcgacc agcgtataag tggggctttt gacattaccc tgatggccca
    14281 aagcctgcaa aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat
    14341 agattacggt tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct
    14401 gctcatcagg gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta
    14461 acaatacgct gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc
    14521 gccgccagta cgtcagctgc cgcaccgtgc gcgacacagc ccgcacaggc tgcatcatac
    14581 ggcgacagtt tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg
    14641 ccgctcgcca tgcctgcatt cccggcatca ataatgccta aagcgtcagg atgggcggcg
    14701 accacggttc cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg
    14761 gcgcaatgta agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc
    14821 tcgccaggat gtggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg
    14881 ttcaatgcat cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct
    14941 tttttccccc actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc
    15001 aggctttcgg taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc
    15061 agcagcggcg caatgttctc actgcgggtc agtactcgga ctaaaccagc accagcacgt
    15121 agcgccgctt cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatc
    15181 accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac gcggtatcag ccactgagaa
    15241 agttgttctg ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc
    15301 cccagtgagt caaaatgcag ttgtccggta acatcccgcg cttttccagt gagcaagcct
    15361 ggtttcagcg caataaaagt gatggtgtga tcggcgttga ttaccgcgcc tggcgtagcg
    15421 ccggtttcag ccagaaggcc ggaagggata tcaaccgcca caatcggcgc agggtgagta
    15481 ttagcgtggt cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtgccg
    15541 agcagcgcat caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc
    15601 tcgcctcccg cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg
    15661 tcactttcct gggccagcag cgtgacatca ataccgatcg ctttggccag ccgcgcgacc
    15721 acgtagccat cgccgccgtt attaccatga ccgcacagca ccagccagtg gcgggcgtca
    15781 ggataagccg aacgacacac ctggaatgcg gcttcgccag cgcgaagcat cagctcatag
    15841 agtgtgagcc ccagcgcatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac
    15901 cagacggtgt gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag
    15961 cccctcgatc tcaatcaatt agcgcaaaat attaaacagt gggggctgga actgggcttt
    16021 cagcaggtag gtattaccga taccgatctc agcgcgtccg agcccaaact gcaagcatgg
    16081 ctggacaaac aataccacgg cgaaatggac tggatggcgc gtcacggtat gctgcgtgct
    16141 cgtcctcacg aattattgcc cggtacgctg cgcgtgatca gcgttcggat gaattacctt
    16201 cctgctaacg ccgcatttgc cagcacgctg aaaaatccca aactcggcta tgttagccgt
    16261 tatgcgctgg gccgtgacta tcacaagctt ctgcgcaacc gactcaaaaa gctgggcgag
    16321 atgattcagc aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt
    16381 ctcgagcgcc cgttagctga aaaagcaggt cttggctgga caggtaagca ctcacttatc
    16441 ctcaatcgcg aggccggttc gttcttcttt ttaggcgaat tgctggtcga tattccgctg
    16501 cccgtggatc aaccagtcga ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc
    16561 ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc
    16621 accatcgaac tggaaggggc gatcccggaa gagttgcgac cgttaatggg aaaccgtatt
    16681 tacggttgcg atgactgcca gcttatctgc ccgtggaatc gctattcgca actcactaca
    16741 gaagacgatt tcagcccgcg taagccgcta cacgcaccgg aactcattga gttattcgcc
    16801 tggagtgaag agaagttttt aaaagtcacg gaaggttcgg cgattcgccg tataggtcac
    16861 ctgcgttggc tgcgtaatat cgccgtagca ttaggcaatg ccccctggga tgaaacgatt
    16921 ttgacagcgc tggaaagtcg taaaggtgag cacccacttc ttgatgagca catagcgtgg
    16981 gcgattgcgc agcaaatcga gaggcgaaat gcgtgcgtgg tcgaagtgca attacccaaa
    17041 aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac
    17101 agcttgtgaa taaaaataaa aacgtattgc aattcaagac gaataaaatc gtcaagtgat
    17161 cgactcgaca aaacgaaatc ttaattttta acttatctat caataggtta aaaaaatact
    17221 attcaccgtg cgaagtttct ttgaaattaa gattacggcc tgaggctgtg gataactctg
    17281 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat
    17341 attttattga gagaaga
//
LOCUS       DAZGTJ010000100        17185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000100 DAZGTJ010000000
VERSION     DAZGTJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.100"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..4515
                     /locus_tag="ACHK8E_004142"
     CDS             <1..4515
                     /locus_tag="ACHK8E_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP0346341.1"
                     /translation="LDNYFGRYDVSTDGKITSGGDFMTEAQRARRVFKDGRYVDAEYP
                     VRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARM
                     AGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVISGIKDSDLKVRVVKSQKEA
                     EALAGESFDGYGKVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPA
                     EYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMVEKQPNKFVAA
                     WERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGVGASSQYS
                     RSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRHLGAPDLPLVIS
                     RDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVV
                     SAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSRDNLLHRGLQLP
                     KGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQEN
                     IRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYL
                     YARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIIDDML
                     ARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQ
                     AMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDT
                     MRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKN
                     MGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKL
                     KGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGWFNMG
                     DLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAG
                     LSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGDGPLL
                     ERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIM
                     KSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPV
                     GSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRS
                     TPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKFWIDYISGGTGRFISKTTDAAVKS
                     LNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDE
                     NNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQVAMVKSVERDMKIAVDRFN
                     REYNKKVGVD"
     gene            complement(4605..4678)
                     /locus_tag="ACHK8E_004143"
                     /pseudo
     CDS             complement(4605..4678)
                     /locus_tag="ACHK8E_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            5447..5620
                     /gene="ymdF"
                     /locus_tag="ACHK8E_004144"
     CDS             5447..5620
                     /gene="ymdF"
                     /locus_tag="ACHK8E_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0346342.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(5703..7031)
                     /gene="rutG"
                     /locus_tag="ACHK8E_004145"
     CDS             complement(5703..7031)
                     /gene="rutG"
                     /locus_tag="ACHK8E_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP0346343.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7052..7546)
                     /gene="rutF"
                     /locus_tag="ACHK8E_004146"
     CDS             complement(7052..7546)
                     /gene="rutF"
                     /locus_tag="ACHK8E_004146"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP0346344.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(7557..8147)
                     /gene="rutE"
                     /locus_tag="ACHK8E_004147"
     CDS             complement(7557..8147)
                     /gene="rutE"
                     /locus_tag="ACHK8E_004147"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP0346345.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8157..8957)
                     /gene="rutD"
                     /locus_tag="ACHK8E_004148"
     CDS             complement(8157..8957)
                     /gene="rutD"
                     /locus_tag="ACHK8E_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP0346346.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(8965..9351)
                     /gene="rutC"
                     /locus_tag="ACHK8E_004149"
     CDS             complement(8965..9351)
                     /gene="rutC"
                     /locus_tag="ACHK8E_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP0346347.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9363..10055)
                     /gene="rutB"
                     /locus_tag="ACHK8E_004150"
     CDS             complement(9363..10055)
                     /gene="rutB"
                     /locus_tag="ACHK8E_004150"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP0346348.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(10055..11146)
                     /gene="rutA"
                     /locus_tag="ACHK8E_004151"
     CDS             complement(10055..11146)
                     /gene="rutA"
                     /locus_tag="ACHK8E_004151"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP0346349.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            11434..12072
                     /gene="rutR"
                     /locus_tag="ACHK8E_004152"
     CDS             11434..12072
                     /gene="rutR"
                     /locus_tag="ACHK8E_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP0346350.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(12112..16074)
                     /gene="putA"
                     /locus_tag="ACHK8E_004153"
     CDS             complement(12112..16074)
                     /gene="putA"
                     /locus_tag="ACHK8E_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0346351.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            16129..16338
                     /locus_tag="ACHK8E_004154"
     CDS             16129..16338
                     /locus_tag="ACHK8E_004154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346352.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            16497..>17185
                     /locus_tag="ACHK8E_004155"
     CDS             16497..>17185
                     /locus_tag="ACHK8E_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HFP0346353.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDML"
BASE COUNT         4165 a         4455 c         4728 g         3837 t
ORIGIN      
        1 ctggataatt attttggtcg ttatgacgtt tccactgacg ggaagattac gtcaggtggc
       61 gactttatga cggaagcaca gcgtgccagg cgcgtattta aagacggcag gtatgttgat
      121 gctgaatatc cggtacgtca ggaggtttac gacgctttta aaggtgtgat tcaggccatt
      181 aaaaacagtg acatgccgcg tcgttcagcg cttctcgatg aggtgcgctc aaaaccgtac
      241 tggtcaacgg atgttgaaat ggcggcacgt gcctttgagc gttatgttca ggataaggcg
      301 cgtatggctg gcgtggagaa tgattatctg gtcaatatcc gtaaggcacc tgagcacaac
      361 acagataaca cctacgctta tccgacgaat gcggaactgg atggcggtat tcgtgaggca
      421 ttcgatcacc tgttccgcac cctgaaaacc cgtgagacgg acaagggagt tgcgttttat
      481 tcccgtaagg gcgtgacccg cacacctgaa ggcaatctga tttcggatgt taaccgcagt
      541 gcggaagcca aaggcagtcc ggtcccgcag gttgaagctg ttgcccgtgg cgtgattagt
      601 ggcattaagg acagtgacct gaaggtccgc gtggtgaagt cacagaaaga ggctgaagca
      661 ctggcgggtg aatcattcga cggttacggc aaggtgcacg cgttctatcg cccggataaa
      721 cgagaaattg tcctggtggc ggataacatc cctgacgggc ggaccgttcg cgagaagtta
      781 cgtcacgaga ttattcacca tgcaatggag catgtagtca cgccagcgga atatcagacg
      841 attattaaga ccgtgctgaa aacccgtgac agtgataacg ccaccatccg tgaagcctgg
      901 cgtaaggttg atgcgtctta tggtaaggaa tcaccggaag tgcaggcggg tgaatttctg
      961 gcgcatatgg tagagaagca gccgaataaa ttcgtggcgg catgggagcg tgttgttgcc
     1021 ctggtcaaag gggtactgcg tcgtacgggg ttactgaagc cgacggaact gaacgatatc
     1081 agacttgttc gcgaaactat tcgcacgtta ggccagcgtg tgcgggaagg ttacacgccg
     1141 cgtgaggatg gcgtgggcgc atcgtctcag tactcccgta gtggtaagcc tgatccgttc
     1201 aaagtgccgg agggtgaggg tgaacgttat cgcgatgacc ttgccagaat gatgaaatct
     1261 ctgcgtacca cagatttaac ggtaaacatc gggcgtacgc cgccggtatt gcgtcacctt
     1321 ggcgcaccgg atttgccgct ggttatttcc cgcgatactg tgcggaaggc caccaatggt
     1381 gtgaaacatg tggtgccgat ggatgttatc gagagactac cggaactgat gcacgatccg
     1441 gatgcaattt accgctcagc gacagaaaga aatgcggttg tgatgctgct tgatgccgtg
     1501 gataaaaatg gtgatccggt ggtgtcagcg gtacacatga aggctgtccg gtcgcgtctg
     1561 gaaatcaaca aggtagcttc tgtttacggt acagaaaatg gaaaaaaact gaagagtatg
     1621 gaaatgaccg ggttaacgtt gtaccggaga gaaaaattaa gccgcgataa ccttctgcac
     1681 agagggctcc aattgcccaa aggggaacat tcttatcgcg gctctgcgga taaaatactc
     1741 tatcctgaag atattcgcaa ggggccgtat tactcccgta ccagcagtct gacaccggaa
     1801 gagacaattg catcgcgttt tgtgcgccag atgcaggata aattccaggt gctgaaagct
     1861 gttcaggaga atatccgtaa aactggcggc aaagtggacg acagtaacaa cgcttatatg
     1921 gcggaagaac tcttccacgg gaaggcggaa aacgacctga acgtgatgaa ggagcgctac
     1981 gttcagccac tggctaaatt actggcggac tacaaaattg cgcaggccga tctggatgag
     2041 tacctctacg cccgtcacgc gccggaacgt aacgcgcata tcgcgaaaat caacccgaaa
     2101 atgccggacg gcggttcggg gatgaccaac gcggaagcgg cggaaatcat gcagcgtgta
     2161 cgtaacagtg gcaaacaggc acagtatgac cgtctggcag ggattattga cgatatgctg
     2221 gcccgtcgcc gtgagcttat ccgtgaggcc ggacttgaag agaacggtgt ggtggatgcc
     2281 tggcagaacg cctaccgtta ctacgttccc ctgaaaggtc aggatgttga cggtgtggtg
     2341 tcactgcccc gtacaggtaa gggcttcacc atcggcggac gtgaaagcag gcaggccatg
     2401 gggcgtgcat cccgggcaca gtctccgtcc actcaggcga tacaggacct gagcgaatcg
     2461 ctgatccgcc atcgcaaaaa tgaagtgggt aacgccttcc tgaagctggt gcaggataat
     2521 cccgataagg attactggca ggtattcact gatgacagac cggataccat gcgtgtgatt
     2581 gcagagcgca aggaccagga aactggtgaa accattcgcg aagttgtcga gcgtccggtg
     2641 gcgatggcaa tgatggcaga ccggtacttc accaccaaaa agaacggcaa aacgtactac
     2701 atcaaactcc atgatccgcg cctgatgcgt gcgatgaaga atatgggacc ggaaaccagt
     2761 aacgccgtaa tccgcacgct ggggaaagtt aaccgcttcc tggcaacggt gaacacgtct
     2821 tataacccgg aattcctggt cagtaacttc atccgtgacg ttcagacggc ggtgatgaac
     2881 ctgaaggcgg agcagggaag gagcgacggc aaactgaaag ggctggataa cttatccgca
     2941 ctggctgtgg tgaaagacag ccggtctgcc atgtctgccg tatacgccag tctgcgtggt
     3001 aaaaccctca cgggcaacgg tgcgcagtgg cagaaggtgt ggaaagagtt tgttgaggac
     3061 ggaggtaaaa ccggctggtt taacatgggt gaccttgaag gccagcagaa ggaaatggat
     3121 cgccttgtat cgctggcgaa gggaggatgg aaaggccaga gtatcggtgc atggaattcg
     3181 ttccttaacc ttgtcgagga tgccaacggg gcggttgaaa acgctctgcg tctttctgcc
     3241 tataaacacg cccgtgatgc cggtttgtca cgccagcagg cggcgtctct tgccaaaaac
     3301 atgacggtga actttaaccg tcgtggtgag cagggagcgc tgatgaactc gctgtacatg
     3361 ttcgccaacg ccagcattca ggggaccgca aacctggtga gaacgctcgg acatcttaat
     3421 ggcgacgggc cgttactgga gcgccttcgc tggaagaatc tcaatgtacc gcagaaaatc
     3481 gcgcttgcag ctgtgggagc gggttatctg cttggctcgc ttaaccgcag tgttgcgggg
     3541 gaggatgatg acggggttaa ctggtatgac aaggtgccgt ctcatgtgaa agagcgtaac
     3601 ctcgtcatta tgaaatcggt gttcgggggc aaggccggag agtactggag tattcctctg
     3661 ccttacgggt acaacgtttt cttcctgctt ggtcacacag ccgaaggggt ggcagcgggc
     3721 gacctgaccg cttcacgtgc tgccggtaat gttgttggtg gtgtgcttgg ggcattcagt
     3781 cctgtgggca gtgaaacgtc ggaaacactg tccggggcat tgctgaaaaa tgcagcgccg
     3841 accattctgc gtccgtttgc gaaccttgcc atgaatgaaa acttcatggg ggcgcagatt
     3901 taccaggaga acatgccgtt tggtacacca aaacctgaca gtcagctggg aagacgttca
     3961 acgccagaag cgtacaaggc gtttgcatcc tggctgaatg cgttctcagg tggcagccag
     4021 taccgtcccg gcgcggtgga tgttacgccg gaatcactga aattctggat tgactatatc
     4081 tctggcggga ccgggcgctt catttccaaa accacggatg cggcggtgaa atcgctgaat
     4141 ggtattgata taccggagca gcaggtgcct ttcctgggga aaatttcagg tgaggtgatg
     4201 ccgtatgcag accagcagaa gatgtacgac cggatgacgg aggttgcgca gtaccacgca
     4261 gagctgaaga gtctgaccgg tgcagaaaga acggcgttca ttgacgagaa caacggaaaa
     4321 ttgtcgatga acgggcttat gcaggatacc cggaagagac tgaaggattt gcgtaaacag
     4381 cgcgatgcca tttatgccga cagttctctc agtctggcgc agcaggtggc gatggtgaaa
     4441 tcggtagagc gggatatgaa aattgccgtg gatcggttta accgcgagta caacaaaaaa
     4501 gtgggagtgg attaacagaa atggccccgt acggaagtgc ggggctaatg tgattgggga
     4561 gggtgaggat acctgacaca tcagaggtgg cgagggattc ctcccttgca gaggtctttc
     4621 ttcgttatca gtgccgtcac aatgacggta atacagagat gagcagggtg attaacatcg
     4681 ccttttgctg ctttcattgc atttctcccg cgatagctca ggcgtatttg tcaatatgca
     4741 gaaatattga tgaacgaatg acggtaaata ccctacccag aaaattaacg taaattcaca
     4801 tcgtaaactt gcgttaatta tctgaaatat atacttaaat ccccttaaat ctttggggcg
     4861 ctattggggc aaagtgtggt tgtttggggc atatttgggg caaaaaagaa cgtataaaga
     4921 acggtgtaaa atcgatttct ttatggtcaa aatgaggtct aacatattga aaagtatatg
     4981 ctcttggacg atcttcggta attctgtgtt tttaatgctt catggtttca atatgtccgt
     5041 acatggaata ataaagcacc agaactttag ccatttctaa ccactcctcg tgttatctct
     5101 attccgtagc gattcgctac cacttattta aagataagac gtccttttca gagtgcaaat
     5161 ttcacaacca cttatttgat ttataacaac tttcacaagc acgtaatttt gtcgcaaaat
     5221 gacacatttt tatctcatcg cgttttttta atcataagag cggcttatgg ataattattg
     5281 gagatgatat ctattctcgc taagaagctg ttgcaggata ttactaaacg cgggtctgcc
     5341 cgcgtcagtt cactaagctt agtcccacgt agcgaaaata tggcagccgc catacgccgc
     5401 gttaattcta tgcaatatga tgtctatacc cagacggagg tcagtaatgg caaaccatcg
     5461 aggcggttcc ggcaattttg cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg
     5521 tggacagcac agcgggggta atttcaaaaa tgatccgcag cgcgcatctg aagcaggtaa
     5581 aaaaggaggc aagagcagtc acggtaaaag cgacaactag ccgggctaat ccatgacgaa
     5641 tgtatttttg tctgaaactc gtcaaaaagt catcaccgcc ggttaccccg gcggttttct
     5701 atttatggct cctgatgaac aacttctggc ggtggaacgt caaccaacct tctgcttaac
     5761 aacgcattga gtaaaatcgc gccaaaggtg gctgtaccga tccctcccaa cgtaaaaccg
     5821 cccagtgtga gagcaaaatc acccgcaccc agcactaagg ttactgcgac cataatcaaa
     5881 ttaccgttct ggcttaaatc cacactgttt tgtacccaga tccgtgcgcc tgcgacggca
     5941 atcagcccga acacaacaat tgatgcacca ccaatcaccg cggccggaat agtatggatc
     6001 agcgcgccaa atttcggcga aaagcccaac agcatggcga tgacggcagc ggcaacaaac
     6061 accagcgtcg agtagacttt tgtcacggcc atcacaccaa tattctcggc gtaggtggtt
     6121 acgccgctac cgccgacaga gccagaaagc atcgtcgcca gcccatcgcc tacgaatgcc
     6181 cgccccatat acgggtccat attgcgtccg gtcatcccgg cgaccgcctt gagatgacct
     6241 aagttttccg ccaccagaat caccgccacg ggcgcaatca gcatcattgc ctgaccatta
     6301 aaagcaggag tggaaaaatg tggcagaccg aaccaggcag catggctgac gagagtaaaa
     6361 tcgacggctt ttcccagccc taaaacgttg gtcatcacgc catacagcag acaggcgaca
     6421 attaatccta cgagaatcaa taatcgctgg atcatgccac gggtaaatac ggccaccagc
     6481 ccaatacaca gcaccgtcat taccgccatc cagctatcaa aggccgaagc cgatacattt
     6541 ttcactgcga taggcgctaa gttcaggcca atcgccatca ctaccgcacc tgtcacaacc
     6601 ggtggcatta gtcgttcaat ccagcgcgta ccgattttca tcaccaccag gccaatgacg
     6661 gtataaacca gcccacaggc gataatcccg cccagcgcga tgctgatatt cggattaatt
     6721 ccctgaccat taaagcccgt cgcggcaatc accacgccga caaaagccgc gctggaaccg
     6781 agataactgg gcacgtgccc gccggtaata aagaaaaaca gtaacgtgcc gatccccgac
     6841 attaaaatgg aaagattggg atccagcccc atcagaattg gcattaacac cgtcgcgcca
     6901 aacatcgcca ccgcgtgttg aacgcccatt actgccgttt gagcaaacgg caatcgttca
     6961 tccggcgcga ccacgccgct ctctgtagag gtcgatttta actgccagtg aggaaaaccg
     7021 aacattgcca tcagctgtct ccttaaggag gttaacaagc agggcgcatc agcgcgtgat
     7081 aactgcgatc gaaccacacc agcccgtagg gtgtggcgtg acgatgaatc gcttcgatgg
     7141 cgcaaaacaa aatgtcgtgg gtgccgacgc tcaccacctg gctgatacgg cagtcaaacg
     7201 aaaccagtgc ctcttccagt tgcgggcatc cggtcacacc agtctgccag cgggcggcgg
     7261 caaagcggtg ttccatgggc gttttgccgc caaaaaggtt tgaaagcggc tcctgcccgg
     7321 cgctaagtgt atttacacac agcgttcgat tttcattgaa taccggccag acggacgccc
     7381 cacgattcag gcacaccagt aatgtgggcg gcgtgtcagt cacgctgcag acggcgctgg
     7441 cggtgaaccc ggcgcgcccg gctggaccgt ccgtagtgat gatattgacc gccgcgccca
     7501 tacaggacat cgcatcgcga aaggtttgtt gatcgacaat gttcattgtt tgctccttac
     7561 aacagcccgc aggcttcttc aaaggacaga cgtggcaggc gcgcaaaaag tttgctggaa
     7621 tcgccatagc cgatattaat cagcagattg ctcttcagcg tgctgcccgc aaaaaaggcg
     7681 tcgtcaacgt attgacggtc aaagcccgac atcgggccgg tatccagccc cagcgcacgg
     7741 caggcgacga tcagataggc cgcctgcatg gaactgttgc gaaacgctgt ttcttcggca
     7801 agttgtggac tggaggtaaa ccaactgcgg gcatcgccgt gaggaaacag ctgtggtaac
     7861 cgttcataaa attcactgtc ccaggcgacg atagcggtga cgggcgcggt cagggttttt
     7921 tgcagattgc cgctggaaag cgccgggcgc agacgttctt ttccttctgc agtgcggata
     7981 aacacgatcc gtgccggaga acagttagct gatgtcggcc cccatttcat cagggcataa
     8041 atctcccgta acgtctcatc gctgacgggt gtctcccgcc agccgttgtg agtgcgggca
     8101 tcggtgaaca gggtgctaag cgcacctggg ctaacggctt cgttcatagc aattccttac
     8161 agagcggctt cacggtgatg taacaggctg gcaagcccgt tgagtaacag agcattaaac
     8221 gtttcgggat cggtcacgtt gcaggcgtgt ccgccatagc gcatcaccat tttctggcta
     8281 tcgggcaggg cggcatgaag ttcactggaa catgctgttg gcaccagcag atcatcactg
     8341 gcgcagatga tttgcaccgg gcagcggata cgatccgcat ggtgactaaa gtcagcgcgt
     8401 ttgagggcgt taagtcgacg cagtaaatta tttttgcctt gaaaatgcgc cagtgccagc
     8461 gcgtcttctg cctcaaggcg aggtgcgcgg gccgccatcc agtcggcggg atagaggaac
     8521 aacggctgcg cttccaccca tgcctgcgcg ccgccgctat gtagtaactg ttcgcgaacc
     8581 tgaaaacagc ggcgcgtatg ggcgtttatt cgtagccagc cgttaacgct gaccagcaca
     8641 gttaccgacg cgggataatc cagcgccagc tgcattccca ccagcgcacc gagcgcatgg
     8701 ccgaccaccg cgtaacgctc aatccctgcg gctaccagcg cctgatgcag ttccgctgcc
     8761 atctgggcga tactgtaatc ttctgccagc gtgtcgggat tattgccggt gccgcgctgg
     8821 tcgtaacaga ctacctgata ctcctgaacc aacaccgcca gttgcggtaa ccagtaactg
     8881 ccgctaccgc caagacccga aatcaacacc actacgggcg catcagcata agggggaggt
     8941 gagagtgaaa gtttcatcgc ggcctcactt ggcgatatgc gcaattgtgg cgatttccac
     9001 cagcgcgtca ggttttacca gtccgcactg aatgcagaat cgcgccggtt tgtcacccgg
     9061 gaaaaattcg gcgtagattt cgttaatcgc ggcgtaattt ttccagtcgg taataaagat
     9121 gctgttgaag gtcacatccg ccattgtgcc acccgccgtc tcgatgacct tgcggatggt
     9181 ttccagaacg tggcgggttt gcgcctttgg gtcatcggca aacagcacgt tgttatgttg
     9241 atcaaaagcc agcgtaccgg agacatacac cacgccatca gccagcgtgc cgggaacgaa
     9301 gggggccagc ggtgcgctgc tgccagcagg aataattacg gattttggca tcgttaaact
     9361 ccttaagcga tacgagcaaa ggacgtggaa gaaagcgcgt cgcagaacgt ttcgacgtcg
     9421 ctgacccagc caaaaaaggt ttcgatattg aacaacgcgg ctttctgtgc aaattccggt
     9481 cccgcctggt gtgttgcgtc ttccagcacc acgccgaaat actccagaaa aaagccgtcg
     9541 cgtagcgtcg attcgacgca gacgttggta gcgataccgg tgaaaaccag atggcgtatt
     9601 ccacggctgc gcaaaatgct gtccagcggc gtattgaaga aaccgctgta gcgcggcttc
     9661 ggcagcacaa tatcgccagg ctgcggcatc agttcatcca ccagttgata atcccaggag
     9721 cctttcgcca gcaatttccc ctgcagctgc ggctgcttac gcatggtttt cagggcgttc
     9781 gatttatgaa aattcggtga gccggggccg ccagcctcga catactgttc atcccagcca
     9841 ttttgaaacc agatgatcaa catccctgcc gctcgcgctg cggtcacggc ggtttgaatg
     9901 ttggcaatga ccgggcgagt ggttgagaca tcaaacccgg cgagatctaa gtagccgcct
     9961 ggcgtggcat aagcgttttg catatccacc acgatcagcg cagtttgctg cggatcgaag
    10021 gtaatggctt ccggtcgtgc ggttaaggtc gtcatcatgc cacctcctga gtcagcgcag
    10081 ggagatgggc gcggcactgc atcagtggtt gaatgcgctc gccgaagttt tcgattcccg
    10141 acagaaaatc atcgaaggtc aacagcacgc cttcggcacc aggcacgctt gcgacttcat
    10201 ctaacatgcg cgcgacactg gcgtaagaac cgactaacgt ccccatattg atgtttaccg
    10261 ccgaagtggg atcggccatc tgacggacgt tggtgtcagt atcggagcgg gtatctttct
    10321 gactttgttc ggttagccag cttaacgcct cttcatccgc gcccgctttg tagtgttccc
    10381 attttgcatg agcggcatcg tcggtttcgt cggcaatcac cataaacaac acataagagc
    10441 caacgtcgcg tccggtttgc tctgcggcct gtttcatccg cgcagcggtc ggggcgaaag
    10501 ccgtgggtgt atttacgcct ttgccgaaac agaagttaaa atcggcatac cgggcggaga
    10561 acgccatgcc agcgtcgctt tgcccggcgc agatcacttt catggggaca ctcggttgcg
    10621 gactgacgcg acaatcatcc atagtgaaaa aatcgccttt aaaatcgctt ttccccgagc
    10681 cccacaggtc gcgcagcacc tgaacatatt cggtgagaga gtcgtaacga cgggagaaat
    10741 agtcatcgcc aggccagatc cccatctgct catactcggg cttctgccag ccagtcacga
    10801 ggttgacgcc aaaacgcccg ccagagatgg agtcgatggt tgcggccata cgggcgacga
    10861 ttgccggagg taacgttaag gtggcagctg tcgcgtatat ctgaatgcgt gaggtgacgg
    10921 ctgccagccc cgccatcaag gtgaacgact caaggttgtg atcccagaac tcagttttgc
    10981 cgccaaagcc acgcagtttg atcatcgaca gggcgaaatc gaaatggtag tgctccgctt
    11041 tttgcacaat ggctttattc agttcaaatg tcggcatgta ctgcggcgcg tgggtcgaaa
    11101 tgagccagcc gttgttgcca ataggtacga atacgccaat tttcatcatc aacctctctt
    11161 cgtctcgtaa agtgaaagtc agacggggcg ctgcatcctg catatccttt tcagccgcgt
    11221 aatggcttgt ttgcaaagcg gatgccagtt tttaaaaagt gaatgttatt aatgtgttaa
    11281 tgttaagttg tctaaaatgc ctgaaaaaaa gtggactaaa cggtcaaaac agttgcacat
    11341 aaaacatgca ttcgtgcgcg atgagagtgc agaaggtcga ggccgggcgg gggttttgct
    11401 atcctgttgc caatcaacaa gaggggagag cgcatgacgc aaggcgcagt gaaaacaacg
    11461 ggtaagcgtt cgcgcgcagt aagcgcgaag aaaaaagcga ttcttagcgc agcactggac
    11521 actttttcac aattcggttt tcacggcaca aggctggagc agatcgcgga gttggcgggt
    11581 gtttcaaaaa ccaatctgct gtattacttt ccgtcaaaag aggcgctgta tatagccgta
    11641 ctgcggcaga ttctcgatat ctggctggca ccgttaaaag cgtttcgaga agatttcgcc
    11701 ccgctggcgg cgatcaaaga atacatccgt ctgaagctgg aagtttcccg cgattatccg
    11761 caggcttcgc gcctgttctg tatggagatg ctggcaggtg caccgctgtt aatggatgaa
    11821 ctgacgggcg atttgaaggc attaattgat gagaaatcgg cgctcattgc cggttgggtt
    11881 aaaagcggca aactcgcgcc gattgacccg cagcatttga tttttatgat ttgggcttcc
    11941 actcaacatt acgccgattt tgcccctcag gtggaggcgg tgacaggcgc gacgttgcgc
    12001 gatgaggtgt ttttcaatca aacggttgaa aacgtgcagc ggattattat tgaggggatt
    12061 cgaccacgtt aaagatgccg gaggaggttg taacatcctc cggctacctg tttaaccgat
    12121 cgtcattaaa ctggcgttac cgccagcagc agcggtattc acactcagcg aacgctcgat
    12181 atacagccgt tccagaagga tattgctttc gccacgggca aaaccctgca ccgaaacaat
    12241 tgcaccatcc cgcgcggcaa cggcttcaca caatgcgcga agctgatccg aatcaccgtg
    12301 gaagatcacc gcatcaaatg gttgagcggt tatattttcc gctttcgcca gttgaatacg
    12361 ttcgctgact gccgatggca atgccttcac taactgacga tgcagcgcat catccggcca
    12421 cagcacctgg ctacccaccg ccagcacggc ggcgagctga gtcagcgcat cctgttcatc
    12481 atccgcaata cacaacacgc gctcacgcgg cagcagcgtc caggtgttgc gttcacccgt
    12541 cggccctggc aacaatcgtt gtgttcctgc ctgcgccaac tcgccatatt gcgtacataa
    12601 cgcctgcaat tctggacggt ttgccgccca ttcccgcagt gcatttagcg gctgagtcaa
    12661 tgcggctttc aactgcgcat cgaccggata ctccgcatcc tgacgcgcga gcgtcactgc
    12721 cagcgcactt tccgggcgat tcgccagcag acggtagaga tagagcggac cgcctgcttt
    12781 cggcccggta ccggacaacc cttcgccgcc gaacggttgc accccaacca ctgcgcccac
    12841 catattacgg ttaacgtaca ggttaccgac atgcgccgag ccagtgacct gggcgatggt
    12901 ttcatcaatg cgcgtatgga cgccaagcgt cagaccataa ccggaagcgt taatctgctc
    12961 gatcagcgcc ggcagctggt tacggttgta acgcaccaca tgcaacaccg gaccaaagac
    13021 ctctttttgt aattcggcaa agtcatccag ttcgatcagc gtcggagcga caaaggtgcc
    13081 gctttgccat tcacgggcat cttcgctgtt ttcccgcacc gcctggaaca cctgacggcc
    13141 tttactacgc atggtctgaa tatgacgctc aatattggct ttcgcttcgc tatcaatcac
    13201 cggaccgata tcggtggtca ggcgacccgg attacccatc cggcattcgg ccattgcgcc
    13261 gcgcagcatt ttcagcgtgt ggtcggcaat ctcatcttgc aggcacagca cgcgcagtgc
    13321 cgaacaacgc tgacctgcac tgtcgaacgc cgaggccagc acatctatta cgacttgttc
    13381 ggtcagtgct gaagaatcga caatcatcgc gttcatgccg ccggtttcag cgatgagcgg
    13441 aatagggcga ccctgagcgt ccaggcggct ggcgatattg cgctgcagta acgtagcgac
    13501 ttcggttgaa ccggtaaaca tcaccccgcg cacgcgatca tcacccgtca gttgcgcgcc
    13561 cacggtttca ccccgacctg gcagcaattg caccacgcct ggcggtacac ccgcttccag
    13621 caaaatggcg atcccttgcg cggcaatcag cggcgtttgt tctgccggtt ttgccagcac
    13681 gctgttacct gccgccagtg cggcagcgat ctgcccggtg aaaatagcca gcgggaagtt
    13741 ccacggactg atacatacca caggccctaa tggacggtgg gtttcgttag cgaaatcatc
    13801 ccgcacctgt ccggcatagt agtggagaaa atcgaccgct tcgcgcactt cggcaatggc
    13861 gttactgaag gtttttccgg cttcacgcac cagaatacca atcagttgct gcatctggct
    13921 ttccattagc acggcagcgc gatgcaaaat cgccgcgcgt tcagccggag gagtggcaaa
    13981 ccagattggc gcgttattaa ccgcactttc cagcgcctgt tctacttcac gcggcgtggc
    14041 ttcacgcaca aagcccacaa tatctttcgg ttccgcaggg ttaataacgg gcgacatctc
    14101 accagccgcg accgattgtt ccagcattgg caaggcctgc catttttgca gtgcactatt
    14161 gagcagggca gaagagagcg aggccagccg gtgttcattg gcgagatcca gccctgccga
    14221 gttgtcgcgc ccgtcaccgt aaagatcgcg cggcagggga attttcggat gcggtaatcc
    14281 agtttgccct tcctgttgcg ccagtttttc tacagcagtg accggatcgg caaccagttc
    14341 atccagtggc aaagaggtgt cagcaatacg gttaacaaac gaggtgttag caccgttttc
    14401 cagcaggcga cgcaccagat acgccagcag cgtttcatgg gtaccgacag gagcataaat
    14461 acgacacgga cggttaagtt tgccgtcggc aactttcccg gtgacctgct catacagtgg
    14521 ctcgcccata ccgtgcaggc actggaactc gtactgaccc gggtagtagt tctgacccgc
    14581 cagttgataa attgccgcca gcgtatgggc gttatgcgtt gcgaactgcg ggtagattag
    14641 attcggcacc gccagcagct ttttcgcaca ggcgagatat gaaacgtcgg tatacacctt
    14701 gcgggtataa accggataac cttcaaggcc gtccatctgc gcgcgcttga tttcactatc
    14761 ccagtacgcg cctttcacca ggcgaatcat cagacggcga cggctgcggg tggcgagatc
    14821 aatcaggtaa tcaatcaccg gtgggcagcg tttttgataa gcctgaataa caaaaccgat
    14881 gccgttccag cctgccagtt ccggctcgaa acagagtttt tccagcagat cgagggagat
    14941 ctccagacga tcggcctctt cggcatcgat gttaatacca atatcgtact gacgcgccag
    15001 cagggtgagt gatttcagac gtgggtaaag ctcttccatt acccggtcat actgggcgcg
    15061 gctgtaacgc ggatgcagcg ccgacagctt gattgaaatg cccggacctt catagatgcc
    15121 acgaccattg gacgctttac caatggcgtg aatcgcctgc tgataggaaa ccatatacgc
    15181 ctgtgcatct gcggcggtca gcgcggcttc gcccagcata tcgtaggagt aacggaaacc
    15241 tttctcttcc agcttgcgtg cattggctaa cgcttctgca atggtttcac ccgtgacgaa
    15301 ctgctcgccc atcaggcgca tcgccatatc cacacctttg cggatcagcg gttcaccgct
    15361 cttaccgata atgcggttca gcgagcggga gaggctggct tcgttatggg tggaaaccag
    15421 tttgccggta aacagcagcc cccaggtggc ggcattaaca aacaacgacg ggctacgacc
    15481 aatgtgtgac tgccagttac cgttgctgat tttgtcgcga attaacgcat cacgggtggc
    15541 tttgtcggga atacgcaaca acgcttctgc cagacacatc agcgccacgc cttcctgcga
    15601 tgacaacgaa aactcctgca ataacccctg aaccatacct gcgcgaccac tggcgttttt
    15661 ttgattacgc agtttatcgg ccagttgata cgccagtttg tgcgcctgtt cagcaactgg
    15721 ctgcggcagg cgagcttgtt ccagcagcat cgaaaccgct tcggtttccg gacggcgata
    15781 ggcagcggtg atcgcggcgc gggaaaccga ctggggcaaa atttgctcgg caaagtcgag
    15841 gaaaggctgg tgtggttcct ctgccggagt cggtgcttca tcgctctcat tggcagcgcc
    15901 agaaagcagc gcaggtagct ccggcagagt atcgctgttt tccagttgtt caagataaga
    15961 aaaaatcgcc tgtttaatta accagtgtgg cgtgcgatcg atacgtgtcg cggcagactt
    16021 aatacgctca cgcgtcgcgt cgtccagctt aacccccatg gtggtggttc ccatgccatt
    16081 actcctgttg ttctgaaagg tgcaacttaa cgttatcgtg aaatatccat gatgttgcaa
    16141 ctttgtgcaa ctgtgttaaa tgtgacctgc gtagcaagct taaaaatgaa tgaaatgtta
    16201 ataaacgaaa tcgatatgac agggattaaa aaaataactc agactttttc tctgcggcag
    16261 ttaacatttt tgaaaggtgc aaccgcaaaa aatgtgagag agtgcaacct gatgaaaaat
    16321 agtgtcgctg agcactaaaa tttaatgtaa atggtgtgtt aaatcgattg tgaataacca
    16381 gcgcttccgg caggatacgg tcgccctggt aaaacataaa ctctgttacc ccgttccggt
    16441 ggcagatata acggcaagtt tcgacattgc cgataataat tttttggaga ctttagatgg
    16501 ctattagcac accgatgttg gtgacatttt gtgtctatat ctttggcatg atattgattg
    16561 ggtttatcgc ctggcgatca acgaaaaact tcgacgacta tattctgggc ggtcgtagtc
    16621 ttgggccatt cgtgacggca ttatcggcgg gagcgtcgga tatgagcggc tggctgttaa
    16681 tggggttgcc gggcgcggtt tttctttccg ggatttccga aagctggatc gccattggcc
    16741 tgacattagg cgcgtggata aactggaagc tggtggccgg gcggttgcgt gtgcataccg
    16801 aatacaacaa taacgcctta acattgccgg attatttcac cgggcgcttt gaagataaaa
    16861 gccgcatttt gcgcattatc tccgcgctgg ttattttgct gttcttcacc atttattgcg
    16921 cttcgggcat tgtggcaggc gcgcgtctgt ttgaaagtac ctttggcatg agctacgaaa
    16981 cggctctgtg ggcgggggct gcggcgacga tcctttacac ctttattggc ggtttcctcg
    17041 cggtgagctg gactgacact gtacaggcca gcctgatgat ttttgccctg atcctgacgc
    17101 cggttatcgt gatcattagc gtcggtgggt ttggcgactc gctggaagta atcaaacaaa
    17161 agagcatcga aaacgtggat atgct
//
LOCUS       DAZGTJ010000101        16833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000101 DAZGTJ010000000
VERSION     DAZGTJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.101"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHK8E_004156"
     CDS             complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHK8E_004156"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0346354.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHK8E_004157"
     CDS             complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHK8E_004157"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP0346355.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHK8E_004158"
     CDS             complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHK8E_004158"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP0346356.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4471..5349
                     /gene="araC"
                     /locus_tag="ACHK8E_004159"
     CDS             4471..5349
                     /gene="araC"
                     /locus_tag="ACHK8E_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP0346357.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5435..6199
                     /gene="yabI"
                     /locus_tag="ACHK8E_004160"
     CDS             5435..6199
                     /gene="yabI"
                     /locus_tag="ACHK8E_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0346358.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHK8E_004161"
     CDS             complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHK8E_004161"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP0346359.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHK8E_004162"
     CDS             complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHK8E_004162"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP0346360.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHK8E_004163"
     CDS             complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHK8E_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP0346361.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9595..9694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHK8E_004164"
     CDS             complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHK8E_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP0346362.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHK8E_004165"
     CDS             11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHK8E_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP0346363.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHK8E_004166"
     CDS             complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHK8E_004166"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP0346364.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHK8E_004167"
     CDS             complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHK8E_004167"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP0346365.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHK8E_004168"
     CDS             complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHK8E_004168"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP0346366.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHK8E_004169"
     CDS             complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHK8E_004169"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP0346367.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHK8E_004170"
     CDS             complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHK8E_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP0346368.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3712 a         4584 c         4584 g         3953 t
ORIGIN      
        1 ttggctgtgg ttttatacag tcattactgc ccgtaatatg ccttcgcacc atgcttacgc
       61 agatagtgtt tatccagcag cgtttgctgc atatccggta actgcggcgc taactgacgg
      121 cagaatatcc ccatataagc gacctcttcc agcacgatgg cgttatgcac cgcatcttcg
      181 gcatttttgc cccatgcaaa tgggccgtga gaatggacca ggacgccggg catttgtgct
      241 gcgtcgattc cctgtttttc gaaggtttct acgatgacgt taccggtttc ccactcatat
      301 tcaccgttga tttctgcgtc ggtcattttg cgggtgcagg gaatggtgcc gtagaaatag
      361 tcggcgtggg tggtaccggt tgccggaatt gactggcccg cctgcgccca gatggtggcg
      421 tggcgcgagt gtgtgtgcac aatgccgcca atggacggga atgcctgata gagcagccgg
      481 tgagttggcg tgtcggagga gggctttttc gttccttcaa ccacttcacc ggtttcgatg
      541 ctgaccacga ccatatcgtc agcggtcatg acgttgtaat cgacgccgga aggtttgatc
      601 accaagacgc cgcgctcgcg atcaacggcg ctgacattgc cccatgtgag cgtgatcagg
      661 ttatgttttg gcagcgccaa attggcttct aatacctggc gtttgagatc ttctaacatg
      721 ttgactcctt cgtgccggat gcgctttgct tatccggcct acaaaatcgc agcgtgtagg
      781 cctgataaga cgcgtcagcg tcgcatcagg cggtgaatgc cggatgcgct ttgcttatcc
      841 ggcctacaaa atcacagcgt gtaggcctga taagacgcgt cagcgtcgca tcaggcgtta
      901 cacacctgat gcggctactt agcgacgaaa cccgtaatac acttcgttcc agcgcagcgc
      961 gtctttaaac gctggcaggc gggtatcgtt atcaatcacc gtgatttcaa tgtcgtgcat
     1021 ctcggcgaac tggcgcatat cgttgaggtt cagcgcatgg ctgaaaacgg tatggtgcgc
     1081 gccaccagca aggatccacg cttcggaagc agttggcaga tccggttgcg ctttccacag
     1141 cgcattcgcc accggcagtt tcggcaggga gtgcggtgtt ttcaccgtgt cgatgcagtt
     1201 aaccagcaga cggtaacgat cgccgagatc aatcaggctg gcgacaatcg ctggaccggt
     1261 ttgggtattg aagatcaggc gggcaggatc gtctttaccg ccaataccga ggtgctgaac
     1321 gtcgaggatc ggtttctctt ccacagcgat cgacggacag acttccagca tgtgggagcc
     1381 gagcaccagg tcattgcctt tctcgaagtg ataggtgtag tcctccataa aggaggtgcc
     1441 gccctgcaga ccggttgaca tcaccttcat gatgcgaagc agggcggcag ttttccagtc
     1501 gccttcgccc gcaaagccgt aaccctgctg catcagacgc tgtacggcca gaccaggaag
     1561 ctgtttcaga ccgtgcaaat cttcaaaggt ggtggtgaac gcgtggaagc caccttgttc
     1621 caggaaacgc ttaatcccca gctcaatacg cgccgcttcc agcacgttct ggcgtttttc
     1681 gccgtggatt tgtgtggcag gcgtcatggt gtagcagctt tcgtactcat cgaccagcgc
     1741 gttaacatcg ccatcgctga tggagttcac tacctgcacc agatcgccaa ccgcccaggt
     1801 attgacggag aaaccgaact tgatctgtgc ggcaacttta tcaccatcgg tgaccgccac
     1861 ttcacgcatg ttatcgccaa aacggcagac tttcagatga cgggtatcct gtttagagac
     1921 cgcctgacgc atccaggagc cgatacgctc atgggcttgt ttatcctgcc agtgaccggt
     1981 aacgacggca tgttgctgac gcatacgcgc gccaatgaag ccgaactcgc gaccgccatg
     2041 tgcagtctgg ttcaggttca taaagtccat atcgatgcta tcccacggca gcgccgcgtt
     2101 aaactgggtg tggaattgca gcaacggttt gttgagcata gtcaggccgt tgatccacat
     2161 tttggccggg gagaaagtgt gcagccacac taccaggcca gcgcaacgat cgtcgtaatt
     2221 cgcgtcacgg caaatagcgg tgatttcatc cggcgtggtg cccagcggtt tcagcaccag
     2281 tttgcagggc agtttcgctt ccgtattcag cgcattaacg acgtgctcgg catgttgggt
     2341 gacctgacgc agggtttccg ggccatacag atgctggctg ccaattacaa accacacttc
     2401 ataattatca aaaatcgtca ttgtcgtgtc cttatagagt cggaacggcc tgggcagcct
     2461 gtgccggggc ggaagttgga agatagtgtt gttcggcgct catcgcccat tgctgatagc
     2521 ggcgataaag ctgttcaaag cgttgtgcct gttcgctgcg cggttgcagg gttttctcta
     2581 ccgcactggc cattttttgc tgggctgagg ggatgtctgc gtgcactttc gcggcgacgg
     2641 cagcgaaaat cgccgcaccg agcgcacagc actggtcaga ggcaacaatt tgcagcgggc
     2701 gattcagcac gtcgcagcag gcctgcataa tgacctggtt tttccgcgcg atgccgccca
     2761 gcgccatcac gttattgacg gcgatcccct gatcggtaaa gcactccatg attgcgcgtg
     2821 cgccaaaggc ggtggcagca atcaatccgc cgaacagcag cggagcgtcg gtagcgaggt
     2881 tcagatcggt aatcacccct ttcaggcgtt ggttagcgtt tggcgtgcgg cggccgttaa
     2941 accagtcgag caccaccggc aggtgatcca gagacggatt tttggcccat gcttcggtca
     3001 gcgccggaag cagttgtttc tggctggcgt tgatttgctc tttcagttcc ggatgctggg
     3061 cggcaagctg ttccagcggc cagccgagta cgcgaccgaa ccaggcgtag atatcaccaa
     3121 acgccgattg gcctgcttcc agaccgataa atccaggcac cacgctgcca tcaacctgac
     3181 cgcaaatacc tttcactgcc cgctcgccaa cgctctgttt gttggcaatc agaatgtcgc
     3241 aggtggaagt accgataact tttaccagag cgttaggctg tgcgcctgcg ccaactgcgc
     3301 ccatatggca gtcaaacgcg ccgccggaaa tcaccacgct ttcaggtagg ccgagacgct
     3361 gcgcccattc cgggcataag gtgcccaccg gaatatcggc agtccaggtg tcagtgaata
     3421 gcggggaagg caaatggcga ttgaggatcg ggtccagctc atcaaagaaa ctggctggcg
     3481 gcaggccgcc ccagctttca tgccatagcg acttatgccc ggcgctgcaa cgtccgcgac
     3541 gaatatcctg cgggcgggtg gtaccggaaa gcagagctgg cacccagtcg cacagctcaa
     3601 tccacgatgc ggcagattgc gccacggcgc tgtcctggcg agtcacatgc agtatttttg
     3661 cccagaacca ttcgctggaa taaataccgc caatatagcg ggagtagtca acgttgcccg
     3721 gcgtgtgaca taagcgggtt atctcttccg cttcttcaac cgcggtgtgg tctttccaca
     3781 atacgaacat cgcgttcggg ttttcggcaa actccgggcg cagcgccagg acattaccgt
     3841 ccgcgtcaat cggtgcgggc gtcgagccgg ttgtgtcaac gccaatcccg accacagctg
     3901 cgcgctgctc ggcgctaagc tctgcaagca cggttttcag cgccgcttcc attgactcaa
     3961 tgtagtcacg cggatgatga cggaactggt tatttggggc atcacaaaat tgccctttct
     4021 gccaacgggg ataccactct acgctggtgg cgatctcttc accggtggcg cagtccaccg
     4081 ccaaagctcg cacagaatca ctgccaaaat cgaggccaat tgcaatcgcc atcgtttcac
     4141 tccatccaaa aaaacgggta tggagaaaca gtagagagtt gcgaaaaaaa gcgtcaggta
     4201 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
     4261 taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg
     4321 gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg ttggattagc
     4381 gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt
     4441 tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg
     4501 ccgggatact cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt
     4561 tatctcgatt tttttatcga ccgaccactg ggaatgaagg gttatattct caatctcacc
     4621 atacgcggcc agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat
     4681 attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa
     4741 tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg
     4801 ccgtcaatat ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc
     4861 agcgacctgt ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg
     4921 ctggcgataa atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg
     4981 ctccatccac cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg
     5041 gcagacagca attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt
     5101 ctgtcgcatc tgttccgcca gcagttagga attagcgtct taagctggcg cgaggaccaa
     5161 cgcattagtc aggcgaagct gcttttgagc accacccgga tgcctatcgc caccgtcggt
     5221 cgcaatgttg gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg
     5281 gccagcccga gtgaattccg tgccggttgt gaagaaaaag tgaatgatgt agccgccaag
     5341 ttgtcataat tggtaacgaa tcagataatt gacggcttga cggagtagca tagggtttgc
     5401 agaatccctg cttcgtccat ttgacaggca cattatgcaa gcattgctgg aacactttat
     5461 tacccaatcc accgtgtatt cattgatggc ggtggtgttg gtagcctttc tggagtcgct
     5521 ggcgctggtc ggtttgattc tacccggtac ggtgctaatg gcgggactgg gggcgctgat
     5581 tggcagtggc gagttaagtt tctggcacgc ctggctggca gggatcgtcg gttgtctgct
     5641 gggcgactgg atctccttct ggctgggctg gcgttttaaa aagccgttgc atcgctggtc
     5701 atttctgaag aagaacaaag cattactcga taaaaccgaa catgcgctgc atcagcacag
     5761 catgttcacc attctggtcg gtcgctttgt tggcccgacg cgtccgctgg tgccaatggt
     5821 ggcgggaatg ctggatctgc cggtggcaaa attcattacg ccgaatatta tcggctgcct
     5881 gctgtggcca ccgttttact tcctgccagg gattctggcg ggtgcggcga tcgatattcc
     5941 agccggaatg cagagcggcg agtttaaatg gttgctgctg gcgacggcgg tatttttgtg
     6001 ggttggtggc tggctgtgct ggcggttatg gcgcagcggt aaagcgactg accgtttgag
     6061 tcattatttg tcgcgcggtc gtttgttgtg gctggcaccg ttaatttctg ccgtcggtgt
     6121 agtggcgctg gtggtgttaa ttcgccaccc gttgatgccg gtgtatatcg atattttgcg
     6181 taaagtggtt ggggtttagg ggatagcctt gtgcgggttg cctgatgcga cgcttgccgc
     6241 gtcttatcag gcctacaaaa cgcactgtcc gtaggtcgga taaggcgttc acgccgcatc
     6301 cgacagtgca tactaacccg taatccccaa tattgccgaa gcactcgcct taccgctcaa
     6361 caactcatcg gtcataccct gccaggcgat gcgcccgtcg gcgaccacca ccgagcgcgt
     6421 ggcgatccgc gccgcatctt ccacactgtg cgacaccatc aatagcgtca ttttttgctg
     6481 ttggcagctc gtgctcacca gcgtcaacat ctcctgacgt aacgccggat cgagcgcaga
     6541 gaacggttca tcgagcaata aaatcggctg ttcgcgcacc agacaacgcg ctaacgccac
     6601 gcgctgtcgc tgaccgccag aaagctcgcc cggtaagcgc gccattaaat tatcaatccc
     6661 catctgacgg gcaatcgcgt gcattttctc ctgttgtgcc gcgttcagct tcaatcctgg
     6721 attaagcccc agcccgatgt tctgcgcgac cgtcaggtga ctgaacaggt tgttttcctg
     6781 aaacagcatc gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccgtcgat
     6841 agtcagcgaa ccgctggctg gcgtcagaaa accggcgatc aaattcagca gggtactttt
     6901 acccgcgccg cttggcccga ggatcgccac ctgctcgctg cgttccaccg ttaagctaaa
     6961 acgcatcggc aaatggtggt aaagccaggt gatatcagtc agttttaaca tttcgccccg
     7021 gtagtttttc aatcacggta aacagcagaa aacagagtag cagcagaatt aacgcggtga
     7081 ccgcgccgtc ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc
     7141 ggaaatcatc gttaccaaac aacgccacca cgccaaaatc accaatcgat agcacacaag
     7201 caaaggccag tgcctgcgcc agtgggcgtt taagggcgcg cagctccacc acttttaagc
     7261 gtgaccagcc ttcaatgccc agcgactgac acaacatact gtaacgcgcg gtgatatcgc
     7321 gcatcgggtt ttccagcact ttcagcgcat aagggatcgc cattaacgca ttggtgaaaa
     7381 tcacaatgcc gtcagcagat tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc
     7441 cggtagccag cacaatcccc ggcatcgcga ggatcaacat gccgctcatc tccagcgcct
     7501 gacccgccag cattttctgc cgcgcccgca gttcgcgact gctccatagc agcatcatgg
     7561 tcagcactac gcataatacg cctgccgcca gcgcaatacg caacgaggtc cacagcgcct
     7621 gccacagcac cggctgcgcc agcacttccg gcaactggcg atttatccca tcgacgagca
     7681 ccgccagtaa cggtggcagc aacagcaaca gcgccagcac aattaacacc gtgtcgcaaa
     7741 tgcggctatg cagacgatcg tccgggtcgc gccagccttg cagcagcgta gtgccgggcg
     7801 caatggcctt actcaatcgc tgactcaaca gcaccagccc aaggcaacac accatctgga
     7861 tcagcgccag catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgataaatag
     7921 ccagctcgat agtcgtcgct tgcggaccgc cgcccagcga caacacggtg gcgaagctgg
     7981 cgaaacagag cataaagata agcgcggcaa ccggcgggat ttgtcgccgt aaccacggcc
     8041 attcgacgaa gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt
     8101 gttccccggg aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggcagattaa
     8161 aaaatacatg cgccagcaaa atgccttgca ggccgtaggg cgaaaacgtc cactccagac
     8221 caagcgattg ccagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga
     8281 aaacagcaac cagcaccggg aggatcaagg tcatggcaca cagacgcaac agcgccagcc
     8341 gtcctggaaa gcgcctgcgg tagagggcgc gggcaaggaa tatcgcgggc acgacagaga
     8401 gcagcgccga gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt
     8461 cctgccagat tgccacccag tcaccctgcg gcgcgttcca ccacagggcg agaaacgcag
     8521 ccagtgcaac cgccaccacc agcgtggcgg cgcttattcc tggaattaac cagccgggaa
     8581 ttaacggctg acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc
     8641 ggctggcgtg aactccagcg tggttgccgg tttggtcaat tgttcaaaac cggcaggcag
     8701 cgtgacgttt gccaccggat acatccagtt gccggttggg atcgcattct gaaaagccgg
     8761 agaaaccata aactggagaa atttttgcgc cagctccggc tgcttgctgg cagcggtgcg
     8821 ggcggcgact tccacctgca gatagtgacc ttcgctgaag tttgcggcgg cgtagttatc
     8881 tttcttctct tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct
     8941 ttcacctttt aaaaacaggc cgtaggcttc gctccagcct ttggtgaccg tgaccgtttt
     9001 cttcgccagt ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccacaa
     9061 cagcaggccc agccccggcg tactggtgcg cggatcctga taaatcaccc gccagttttg
     9121 atcgctctca acaagctctt tcaggctttg cggcggattt ttcagtttgt tcttgtcata
     9181 gacgaaggcg aaatagccgt aatcaaacgg tacgaaagtg tcatttttcc agccgccggg
     9241 aacgttaacg gcatccgctg ccacgccgct tttggcaaat agtccggttt tactggcggc
     9301 gtctaacagg ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat
     9361 ccgcagacgg ttgagcagcg aaacgccatc ttccagcgcc accagtttca gttcgcagtt
     9421 acagtcggct tcaaaagctt ttttgatctt tggaccaggc ccccagtcgg cggcgaagga
     9481 atcgtaggta taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag
     9541 caaggggaga cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga
     9601 aggagcctca aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag
     9661 cgcacgcgtc tgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa
     9721 ccagcaatca tggatccggc ggcgcaaacc acgctgattt aaaatcgaac cagccgaggg
     9781 tattcatacg caggccgcgc atactgcgtt gcccctgaat gatcagccag tggtggataa
     9841 gcggcaccat tgctttgctg gcgaccagtt gctggcacca gttcgccagg tttatttcgc
     9901 cattgcgcca gcgggcggcg tcggcttgcc agtcgatcgg aatacagtgt tgtagcagcg
     9961 gcacctcgca caggtgcgcg aatagcgaaa aatccagcgg cagcgtaaag ttggcgctgt
    10021 taagccagat atcgctctcg atctctcctt catgccactg atcgtagctg atctctttga
    10081 tttccagcgt gacctggtga cttgccagaa tctgctgcat gatcccggca atcaccctat
    10141 gctcactgtg atcctgataa aaggtcaggg tgaggctttc cagaccagcc ggtttttcgc
    10201 tctttatggt gcgggtatgg tgccaacggg gaagcagccc ataggccgga aaccacaact
    10261 gctggtactg ttcctcggcg aaataaacca gattggtcgg cgaaagcaca tagcttatcc
    10321 agtctctgac ttgctgattc gccccgcgat gggtacggct gtcgaacagt aaatagtaac
    10381 aaccttcttc aaggcggctt tcaatctctt tttcctcgtc ttgcggtcct ttcagcatca
    10441 ggcctcccgc ggggtcatca gcaatttccg gcaagaccca gacgttaact tcgtcgatta
    10501 atgcccgata accaaagaag tcgtcgaatg cctgaatttt cagttgattg ctgctgttgc
    10561 ggatcaccgc ataaggaccc gttccgatgg gatggctggc aaagttgctg agggtttccc
    10621 attcgcgcgg caggatcatc gccggaactt gccccagcag taacggtaac cagcggtccg
    10681 gttgcgtgag atggatatcc agcgtccagg gcgtcggcga cacaatgtca gcaatatgcg
    10741 aatagagcgg cagcgtattg attcgtttta aagaggcgat cacgtcgtcc atttccagtt
    10801 cacgaccatg atgaaaatgg actcctggac gcaaaaagaa acgccagtga agcggtaaaa
    10861 tttgctgcca gtggtgggcg atgtccgctt ccagttcccc attttcctca tttatgcgcg
    10921 ttagcgaact gaagatttgc cgggcgatat gggtttcgga acgacgcaat gcactgccgg
    10981 gcagcagatt acgcaacgga cgatagtaga gaacgcgcag gatgtgccgc ccctggcgga
    11041 agctgcgtcc cagatgagaa accaacattt gccgcacagc cgctttgtcg ccaaccagtt
    11101 gcaccagttg atcgatacga tcctgctcca gcaggtcctc tgctcgctgt tgctgaagcg
    11161 ccagcccggt atagaggaat gtcagacgcg agcgtttgcc gcgcccgact tccgcttccc
    11221 acgtcagcca gccgcgatcc tgcatggtgt tgagcagggt gcgcatatga cgacgcgagc
    11281 agctcaataa cgctgccagt tcgttgagcg tcgtgtcctg cgatttaccc tcgcagcatt
    11341 gccacaggcg gatgaactgt tgttgcagac gagcagatgg cataaaaggg gaactcctgc
    11401 gcaaaagaca gcaattttat tttccctata ttaagtcaat aattcctaac gatgaagcaa
    11461 ggaggtgcct catgcgtcag ttttatcagc actattttac cgcgacagcg aagttgtgct
    11521 ggttgcgttg gttaagcgtc ccacaacgat taaccatgct tgaagaactg atgcaatggg
    11581 aggggagtca ttctgactac tgatttgcgg acatcatgtg tgactgagta ttggtgttaa
    11641 tcgccacgcc agcagtgatt atctgctggc ttttttcttt ttggcgattg agtgccggac
    11701 ggtgtacaaa ctaacgcata aacgccggtt gcttctcttc ataagacgca attgcgtcgt
    11761 cgtgctgcag agtcaggccg atgctgtcca gaccgttcat catgcagtgg cggcggaagg
    11821 catcgatggt aaagcgataa gttttctctc ccgctttcac ctcttgcgct tccaggtcta
    11881 catcaaaatg aatccccgga ttggcctgca ccagcgcaaa cagttcgtcc acttctgcgt
    11941 cgcttaattt caccggcagc agctggttgt taaagctgtt gccgtagaag atgtcagcaa
    12001 aactcggtgc aatcaccact ttaaaaccgt aatcggtcaa tgcccacggc gcgtgctcac
    12061 gcgaggaacc gcagccgaag ttttctcgtg ccagcaaaat ggaagcgccc tgatactgcg
    12121 ggaagttcag cacgaagtcc gggtttggct gttggccttt ttcatccaga aaacgccagt
    12181 cgttaaacag atgcgcgcca aaacccgtac gggtcacttt ctgcaaaaac tgtttcggga
    12241 tgattgcatc ggtatcgaca ttggcggcat ccagcggaac caccaggcct gtgtgtttga
    12301 taaatttctc tgccatggtg tgctccttat ttaatgttgc gaatgtcggc gaaatgtccg
    12361 gtcacagcag cagcggcagc cattgccggg ctgaccagat gggtgcgccc tccacgcccc
    12421 tggcggcctt caaagttacg gttgctggtg gaggcacaac gctcacccgg attcaggcga
    12481 tcgttgttca tcgccagaca cattgagcaa cctggcaagc gccattcaaa accggcttca
    12541 ataaagattt tgtccagacc ttccgcttcc gcctgggctt ttaccgggcc agagccggga
    12601 accaccagtg cctgcacgcc tggcgcgact ttccgtcctt tggcgatttc agctgccgcg
    12661 cgtaaatctt caatacgtga gttagtgcag gaaccgataa acactttgtc gatagccact
    12721 tctgtcagcg gaatacccgg tttcagcccc atataggcca gcgctttttc tgccgacgcg
    12781 cgttcaaccg gatcggcaaa cgaagccgga tcgggaatgt tgtcgttcac ggaaatcacc
    12841 tgacctggat tggtgcccca ggtgacctgc ggtgaaattt cttctgcctg caaagtcaca
    12901 acggtatcga aagttgcgcc ttcgtcggtt tgcagagttt tccagtaggc aacggcgtcg
    12961 tcgaaatctt tgcctttcgg cgcatgcaga cggcctttga catagttaaa ggtagtttcg
    13021 tccggtgcaa ccagaccggc tttcgcgccc atttcgattg ccatattgca cagggtcata
    13081 cgaccttcca tgcttaaatc acggattgct tcgccgcaaa actccaccac atgcccggtg
    13141 ccgcctgcgc taccagtttt accgataatc gccagcacga tatctttcgc tgtaatgccc
    13201 ggcgcggctt tgccctggac ttcaattttc atggtcttcg cacgaccctg tttcagggtt
    13261 tgcgttgcca gtacgtgttc aacttcggaa gtgccgatac caaaggctag tgcgccaaac
    13321 gcgccgtggg tggcggtatg cgagtcgccg cagacaatgg tcatccccgg caatgtgacg
    13381 ccctgttccg gccccattac gtggacgatc ccctgatacg ggtgattcag gtcatacagc
    13441 tcgacgccaa attctttgca gtttttgatc agctcctgca tctggatgcg cgccatttca
    13501 ccgcaggcat taatgtcttt ggtctgagta gagacgttgt gatccatagt ggcgaaggtt
    13561 ttgcctggct gacgtaccgg gcgaccgtgg gcgcgcagac catcgaacgc ctgcggtgag
    13621 gtcacttcat gcaccaggtg gcggtcgata tataacagcg gggtttcgtt ttcggcttcg
    13681 tacactacgt gagcgtcgaa caatttttcg tataacgttt tagccatgat tacacccctt
    13741 ctaccacata gcgggcaatg atatcgccca tttcattggt actaacggcg gcagcgccac
    13801 gggctaaatc cccggtgcga atgccttctt ctaatgcgcg gttaatggcg cgttcaatgg
    13861 cgcaagccgc atcatctgca tccaggctgt agcgcagcag cagtgccagc gacagaattt
    13921 gcgcaatcgg gttggcgatg tttttgcctg cgatatctgg tgctgagccg cctgccggtt
    13981 catacaaacc aaaaccttgc tcgttcaggc tggcggaagg caacatcccc atcgagccag
    14041 tgatcattgc gcactcgtca gacagaatgt cgccaaacag gttggaacac agcaggacgt
    14101 caaactgtga tggatcttta atcagctgca tggtggcgtt gtcgatgtac atatgcgcca
    14161 gttcgacatc cgggtattcc gtggcgatct cgttgacgat ttcacgccat aaaatagagg
    14221 attgcagcac gttggcttta tcgatcgacg tcactttatg gcgacgttta cgagcagatt
    14281 caaacgcgat gcgggcaata cgctcgatct caaaacggtg atacacctcg gtatcaaacg
    14341 ctttttcata ttgtccgcta ccttcgcggc cttttggctg accgaaatag atgccgccgg
    14401 tcagttcacg cacacacagg atgtcgaagc cgttagcggc aatgtcagca cgcagtggac
    14461 agaatgcttc cagcccctga tacagttttg ccggacgcag gttgctgaat aatttgaagt
    14521 gcttacgcag aggcagcagc gcgccgcgtt ctggttgctg gtctggtggt aaatgttccc
    14581 acttcggacc gcctaccgag ccaaacagca cggcatcggc ttgctcacaa ccttcaaccg
    14641 tcgcaggcgg cagtggttgc ccgtggttat caatggctgc gccgcctaca tcgtaatggc
    14701 tggtggtgat acgcatcgca aatcggttgc gcacggcatc cagcactttc agcgcctgag
    14761 tcatcacttc cggaccaata ccgtcccccg gcaatacggc aatatggtaa ttcttcgaca
    14821 tcacacggtt tccttgttgt tttcgttgtg ttgagctttg cgttgcaact ctttttcgac
    14881 ttctgcggca cgccagatat tgttcagtac gtgcaccatg gctttggcgg aggactcgac
    14941 aatatcggtg gccaggccga cgccgtggaa gcggcgaccg ttgtagttgg cgacaatatc
    15001 cacctgaccc agcgcatctt taccgtgacc tttggcggtc aggctgtatt tcaccagttc
    15061 gacgttatag tcagtgatgc ggtttatcgc ctggtagacg gcatcgaccg gaccgttacc
    15121 gttggcggct tctgctttga cttcttcgcc gcaggccagt ttgacggcgg cagtggcaat
    15181 atcgttagaa cctgactgca cgctgaagta atccagacgg aaatgctccg gctcttcttg
    15241 ctgcttaccg atgaaggcca gcgcctccag atcgtaatca aacacctgac cttttttgtc
    15301 cgccagcttc aggaaggcat cgtacaaatt gtctaaatta tattcacttt ctttataccc
    15361 catctcatcc atgcggtgtt ttactgccgc acgccccgaa cgagaggtca gattcagctg
    15421 gatttggttc aaaccaatag attctggtgt catgatttcg tagttttcgc ggtttttcag
    15481 cacgccatcc tggtggatac cggaggagtg tgcgaatgcg ccgctgccaa caatggcttt
    15541 gtttgccggg atcggcatat tacaaatctg gctaactaac tggctggtgc gccatatctc
    15601 ctggtgatta atagcggtgt ggacgttgag gatatcctta cgaactttga tcgccatgat
    15661 gacttcttcc agcgaacagt taccggcacg ctcgccgatc ccgttcattg caccttccac
    15721 ctggcgtgca ccggcatgta ccgccgccag tgagttgccg accgccaggc ccagatcgtc
    15781 gtgggtatgt acggaaataa tggctttgtc gatgttgggt acgcgttcat acagcccgct
    15841 gatgattccg gcgaactcaa atggcatggt gtagcccacg gtgtccggaa tgttgatggt
    15901 ggtggcaccg gcattaatcg ccgcttcgac cactcgcgcc agatcggcaa ttggggtgcg
    15961 tccggcatct tcgcaagaaa attcaacatc atcggtgtaa ttacgggcgc gtttcaccat
    16021 atagatagcg cgttcgatca cttcgtccag cgtgctgcgc agcttggtgg cgatgtgcat
    16081 tggcgaagtg gcaataaagg tatgaatacg gaaggcttcg gcgactttca gggattcggc
    16141 tgccacgtca atatcttttt ccacgcagcg agctaacgcg catacacggc tatttttaac
    16201 ctggcgagcg atggtctgca ctgattcaaa atcacccggc gaagagacgg ggaaaccgac
    16261 ttccatcacg tcaacaccca tacgctcaag ggccagcgca atttgcagtt tttctttcac
    16321 actcaagctt gcctgtaacg cctgttcacc gtcgcgcaat gtggtatcga aaataatgac
    16381 ttgctggctc atggtttggg tccttgtctc tttttgagcg cctcgcttcg ggcataaaaa
    16441 aacccgcgca atggcgcggg ttttttgttt gactgagtgc tgacttaatt ctgaatgccg
    16501 ctcaccagtc taccgcgcaa agaagatgcg tttagtagta gtagaccgat aaagcgaacg
    16561 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggatacc
    16621 aaaacggatg tcaaccctga tgcaacaaaa acgtccagcc agcgtgagtt ctgcatccgt
    16681 caaattagct aattgtgctg cggtggttaa aacaagcgat attaatttct gcttaaccac
    16741 cgccgctttt catcggttga catatttcag aataaatttt tgcatctaat cgacgaggga
    16801 aagggggata aaatgcacgc gttacaaaac cta
//
LOCUS       DAZGTJ010000102        16735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000102 DAZGTJ010000000
VERSION     DAZGTJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.102"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(280..1488)
                     /locus_tag="ACHK8E_004171"
     CDS             complement(280..1488)
                     /locus_tag="ACHK8E_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP0346369.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPGTNSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTLITG
                     MDNQIFSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPSDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELPGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPGAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRN
                     LKAIYQAPTEEAGQQAQEAFAASWDSRYPHISRSWQANWPNLATFFAYATGIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     DFGDRLGGHF"
     gene            complement(1537..2831)
                     /gene="insG"
                     /locus_tag="ACHK8E_004172"
                     /pseudo
     CDS             complement(1537..2831)
                     /gene="insG"
                     /locus_tag="ACHK8E_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2945..3520)
                     /locus_tag="ACHK8E_004173"
     CDS             complement(2945..3520)
                     /locus_tag="ACHK8E_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0346370.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(3588..4166)
                     /gene="terD"
                     /locus_tag="ACHK8E_004174"
     CDS             complement(3588..4166)
                     /gene="terD"
                     /locus_tag="ACHK8E_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP0346371.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4215..5255)
                     /gene="terC"
                     /locus_tag="ACHK8E_004175"
     CDS             complement(4215..5255)
                     /gene="terC"
                     /locus_tag="ACHK8E_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP0346372.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            complement(5274..5729)
                     /locus_tag="ACHK8E_004176"
     CDS             complement(5274..5729)
                     /locus_tag="ACHK8E_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0346373.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            complement(5752..6909)
                     /locus_tag="ACHK8E_004177"
     CDS             complement(5752..6909)
                     /locus_tag="ACHK8E_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0346374.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6909..7490)
                     /locus_tag="ACHK8E_004178"
     CDS             complement(6909..7490)
                     /locus_tag="ACHK8E_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0346375.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            7810..8868
                     /locus_tag="ACHK8E_004179"
     CDS             7810..8868
                     /locus_tag="ACHK8E_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP0346376.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            8878..10020
                     /locus_tag="ACHK8E_004180"
     CDS             8878..10020
                     /locus_tag="ACHK8E_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP0346377.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            10013..10786
                     /locus_tag="ACHK8E_004181"
     CDS             10013..10786
                     /locus_tag="ACHK8E_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346378.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            10788..11867
                     /locus_tag="ACHK8E_004182"
     CDS             10788..11867
                     /locus_tag="ACHK8E_004182"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP0346379.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            11867..12823
                     /locus_tag="ACHK8E_004183"
     CDS             11867..12823
                     /locus_tag="ACHK8E_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP0346380.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            12834..13929
                     /locus_tag="ACHK8E_004184"
                     /pseudo
     CDS             12834..13929
                     /locus_tag="ACHK8E_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13947..14414
                     /gene="terW"
                     /locus_tag="ACHK8E_004185"
     CDS             13947..14414
                     /gene="terW"
                     /locus_tag="ACHK8E_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP0346381.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            14783..15436
                     /locus_tag="ACHK8E_004186"
                     /pseudo
     CDS             14783..15436
                     /locus_tag="ACHK8E_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(15497..>16735)
                     /gene="fabV"
                     /locus_tag="ACHK8E_004187"
     CDS             complement(15497..>16735)
                     /gene="fabV"
                     /locus_tag="ACHK8E_004187"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010177.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HFP0346382.1"
                     /translation="NLNLFNYQGPYMIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQS
                     HLQQINNGPKRVLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSEKSIGTAGWY
                     NNIFFKEFAEAEGRIAHNVIGDAFSDDVKNKVLNLINKDFDGEVDLIIYSLATGQRSD
                     PATGNIWRSCLKTTGSAFTGKTVNIEDNKLESTIIDVASEAEIADTIKVMGGDDWESW
                     ILWLKENNVLTKKTKTIAFSYIGPEMTYPIYHTGTLGKAKQHLHETSDRLNKLISTDK
                     HSGAYVVVCKALVTKASVYIPCFSPYILCLYKVMKSMGLHETCIGQMHRLFTEYLYNV
                     DNVVVDSSRLIRIDDLELREDVQQKVIDLMSKMNDENFTSVGDYIGYKKEFLQLNGFG
                     FESVDYNHPVDIDYLMNLKP"
BASE COUNT         4279 a         4253 c         4018 g         4185 t
ORIGIN      
        1 tggtttattg cccggggttt cagggctgaa aggatacatg caacgggccg tggatatgat
       61 gcaccagtgg tgttctgccc ggatgtggag ggcgaagcac tgaaggaatg tctgcgcccc
      121 aaccgcaggg ttgaaataac cggggaaaca tcagtgactg attaacaata aagtgcccca
      181 ggagccaatg ccagtcagtt cagcaactga ctggctcttt ttcggggctg tggggtattt
      241 ccagggagcc tgtttaggaa tctgtgtaaa tgccctttct cagaagtgac cacccaggcg
      301 gtcaccgaag tcgataataa agcggctcat tgccatacgc cagtccttca acggcatcgt
      361 ccatttctgg gacgctgact ggattgccag ccataccact tttttcaccg agtcgtctgt
      421 cgggaacact ttacgctttt tgatcgcatg gcggatcacg ctgtttagcg actcgatggc
      481 attcgtcgta tagatcactt tgcggatgcc cgtcgcataa gcgaagaacg tggcaagatt
      541 cggccagtta gcctgccagc ttcggcttat atgaggatag cgactgtccc aggacgcagc
      601 gaacgcttcc tgtgcctgct ggccggcctc ttccgtggga gcctgataaa tcgctttcag
      661 gtttcgagtg acggctttgt agtccttcca tgacacgaag cgcaggctgt tgcgcaccat
      721 atgcacgatg cataactgga tgcgggcctt cggatatact gtgttgatgg cccccgggaa
      781 gcctttcagg ccatccacac aggcgatgag gatatcgttc agaccgcggt ttttcagttc
      841 agtcagcaca ttgagccaga acttcgcccc ttcattttcg gccagccaca tacctggcag
      901 ttctttctga ccttcgatat tgatgcccag tgccaggaac accgatttgt taatgacgcg
      961 actgtcctgc cgaactttaa ggacgataca gtcaagataa acaatggggt aaacagcatc
     1021 cgacggtcgg ttttgccatt ctacaacctg ctccatcacg gcatcggtga cctttgatat
     1081 cagtgccggt gaaacatctg cgtcatacag ctctttgaac gcagcagcga tctcacgggt
     1141 ggtcatccct ttggcataca acgagaagat ctggttatcc atcccagtaa taagggtctg
     1201 atttttcttt accagttgtg gttcgaaggt accgtcacga tcgcgcggag tacgcagttc
     1261 cagtggaccg tcgcctgtga taacggtctt tgtggaataa ccgttgcggg agttagttcc
     1321 cggtttggac tgatttttct catacccaag atggtgtgtc atctctgcat tgagagcggc
     1381 ttcaacgctg atctttttca gtagacggtc aaactggctg aggtcttcag gggttttgag
     1441 gtttttggcc agttcgttag ccagagcctg taactgtttt tcgtccataa attaaccttc
     1501 atttgatgct tgattgaaca tatcaaaatc aggcaattac acaaatctat gtacaggctc
     1561 tttccaaggc ctctccttta ccactctcgg gaagcccctt tcccttcttg tcggtaattt
     1621 cacaagttgt cccatacttg caagatcgcg catcagcccc ggtatacgtc ccggtgaagc
     1681 gccctgcaat gtcatcacca ttccgcatga ttctgagaaa ctcagttgat tcggccagta
     1741 acctttcaga tgttccacca ttttaatcat ctgacatctt accagattat aagccagtaa
     1801 gacaccccac agctcttgct ccacaagctc cggcttttta cagcgtcagc ctgctcagtt
     1861 gcatcgtctg ttttatctcc ctgtatccca gttcaatttc ccggcgatga ctgtacagat
     1921 ccgccatttc tcccccgggg aagcgcatgg cgtccgtcat cgacgtcagc agatggcaga
     1981 cttctccttt gcgcgtcacg gtcagcaggc gggctgtcac ctcatttccc agtcccggcc
     2041 acttttttcg tgcctgcgga ctggttttca gcttcaccag atgatcgcct ttacccagtt
     2101 ttctgatctc ttcatattgc gctccctttc tgagagggat catccagtgg cggtgttctc
     2161 ccaccaggct ccaggcattt aacagtccca gtgagtaata acctttatcc attaacgtca
     2221 gggtgttatc gccggtttgt tctataagtt gctcagcaag ctcattttcg ctgttcttca
     2281 tcgtgccgaa ggctcagccg taagcagatg gctggtcagt tccatctggc agactatttt
     2341 gacctgcggg tagagcgccg ggttgccggc atgtatctgg cgggggaagg ctgcatcgtt
     2401 ctctggtgta tccggtgtgc gccagaacat accatcgatg gccagcaggg tcaggccgca
     2461 tcagtgcgga tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga acacgcggcg
     2521 gacagcctca cttcccaggt gctggcgggc ctgaataacg gcactggggg caacgaaggg
     2581 gcgattgccg gcagcatgat gtccaggcga ttcacaatct ggtgaagggg ttctttacgc
     2641 tcaagcgcca tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt
     2701 agcgttaccg tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc
     2761 cccagagaag ccagtgggtt acgcagagaa tcgtaacggg ataccagatc aagggcctgt
     2821 ccagtgtgca taaaaaatcc ggaaacgagt gagcgtttcc ggattcttac acaaccactg
     2881 gatcggtcaa ctgatcctta accgatcggc attacggcat tgccggggtc agactaagca
     2941 tcttttagac gttgacgccg tgctgggaag caagcgctga caggccaccg gcaaaaccct
     3001 gaccgacagc tttaaacttc cactcagcgc catggcgata cagttcaccg aagaccattg
     3061 cggtttcagt tgaggcgtct tcagacagat cgaaacgggc aatttccgcg ccgttatcgt
     3121 tgttgtaaac gcgcatgaag ctgttgctca ccatgccgaa attttgttta cgcgcttctg
     3181 catcatagat ggtaacggca aataccagtt ttttgacgtc aactgagact tttgccagat
     3241 cgattttaat ttgctcatcg tcaccatcgc cttcaccggt acggttgtca ccctggtgct
     3301 ctactgcgcc atcagggctg gttttattat tgaagaaaat gaaatgggca tctgacagca
     3361 ctttaccgtc ttcacctact gcgaatacgg aagcgtccag atcaaaagcc tgaccgtcag
     3421 ttacacgggc atcccagcca agaccaacca tagcaacatt catggtcggt gcttctttgg
     3481 tcagagatat gttgccgcct tttacgagag aaactgccat ttttagctcc tgcaaacagt
     3541 tgaatgaggg ctgaatcaac agcccggaat gaaaagttag ttttcgatca agacgcgtta
     3601 atgccgtact gagcacatac agatgccagc ccccccgcat aaccctgacc tacagcacgg
     3661 aatttccact caccattgtg acgatacagt tcaccgaaca gcatggcggt ttcagtggac
     3721 gcatcttcag tcagatcgta gcgagcaact tcagtctggt tatcgtcatt aaccaggcga
     3781 ataaacgccc cggaaacctg accaaagttc tgacggcgag cctgagcatc atggatcgtc
     3841 acaacgaaga tgatcctgtc gatgtcagac gggacagtat caagtttaat tttcagcgat
     3901 tcatcatcac catccccctc accagtacgg ttatcgccgg tgtgcgttac agaaccgtcg
     3961 gatgacgtca ggttgttata gaagatgaaa tctgaatcgc cacgcacttt gccgtttgcg
     4021 gtcagcagga atgctgaagc atccaagtca aagtcctgac cgtcagttga acgtgcatcc
     4081 cagccaagac ccaccaggac gtttttcatt gacggagctg ctttactcag ggagacgttc
     4141 ccgcctttgg aaagagaaac actcataaaa taacctcttc gattagtaat tgctccggtt
     4201 aacacttaag gggatcagtt ccccttttcc tcagattcag gtttgccggg gaaaataacg
     4261 cttgcgagaa tgcccagcac cagaaccccc agtacaacat acaggctggt tgtcgcagcg
     4321 atgctgtaac catgatgcca gatgtgatcg gtcgcgttca ggcccagttt taccgcaatg
     4381 aagaacaaca acacgataac agccttttcc agatgaatca ggtactgttt cagtgcttca
     4441 aggacaaagt acagagtacg cagccccagg atagcaaaca tcatggcact atagacgata
     4501 agcggttcac gactgacagc aatgattgcc ggtaccgagt caaacgcaaa cataacatcc
     4561 gagagttcaa ccacggccac acacaggaac agaggggtcg catacagcgc cgccttttta
     4621 ccacggtcaa tggttacatc actgttttct ggtttcgcca gttcagcatc cacttccttc
     4681 tggttaagca ggaaggcatg cccacgtagc ttcggccaga cagggaagaa acgtttaacc
     4741 atgcggtaag ccagatgctg ggagtaatct tcaatttcat catcatcatc accgctttta
     4801 agcatcatga ccgctgtcca ggcaacgatg atagcgaaga cgacttcaac gtacggcccc
     4861 agactcagca ggctcgtacc gatggcgaca aagattcccc tgaagacgat cgctccaatg
     4921 atcccccagt aaagcacacg gtgccgataa cgatccggaa cagcgaacca ggaaaagatg
     4981 gccatcatga caaacaggtt gtcgaccgac agtactttct ccagcgcata ccccgtgaca
     5041 aacaaactgg caacttcagc accgtggtgg atatagagga aaccggcaaa tgccatcgca
     5101 acaacaaccc agaatacgga ccagagcgct gcactcttca gggagattgg cttgtcatca
     5161 cggtgcataa acaggtcaat aaagatggct ccgactgaaa gagcaataaa aacaatgacc
     5221 gtttcagtcg ggaagccgat gtgtgtggaa accatatttt acccttaggg atgtcagagg
     5281 ccaaattcag ccggctcgaa gcccaacgcg atagcgatct cgcgggaggc cagtttctcg
     5341 tcctgatcga agttaccgtc gcttttcgca acagcaatac caacacgtaa ggccagttga
     5401 gcggcctcag gctgatcttt cagcgccagg atgtatttca tggtctcgcc cttgccgatt
     5461 tcaacatcga aatcgaagct tgaaaccagt ttattgaaga actcgatcac ctcattggta
     5521 tcgaagacct tcagctcttc tgaagagcgc agaaagccca tcattttctg cttttcttcc
     5581 gaactcacgc cgtcactcga tacggcaata cgggcacata cagcaacggt gccctgcatg
     5641 aattttttgt ttttgaaacg accaacctgg cgggtcagtt cgtcacggcc agagttaatg
     5701 gcacctttaa ctttgtcgaa aaagctcata ccagtttcct ttattgctgt gttatttaga
     5761 accggcgctc cagcgaaatc cccagccaaa tgcccggtcc atttcgtcct ggcctttgaa
     5821 gtactggttg atacgctcga ctttaatcgc accgttttcg tttacgagtc tggcaatggc
     5881 gcacaatgtg cgacggtttt caccttcagt caggcgggtc tcaatgggcg gttgatccgg
     5941 cacataaatg gtgaccacac catcagtctt gtcccaactg ggaacacctt cataaataaa
     6001 ggcgtaaatc agcacttcgc ggatatgctt ccattcacgt ccattgatat gcagccactc
     6061 gccgtctgat acatcacccg tccggtcatc gcccttgagc tgaacataag gttcatcgcg
     6121 ataatcaccg aatgcattac cgagcgcctg aatgaccgac ttatacccgt cctgaagctc
     6181 aacaaaggct cccagatcca ggtcgatgcc tttggagccg aacatgcctg caaagccgga
     6241 tttgccgctg ccccggtgcc agttcaggtt gatcctgatt tcaccgaagt tatcccgttt
     6301 ggtcagactg attgcaggca tctcttttgt cagagatact ttactcaggc tgatttttgt
     6361 ctcagctggc agagggggta caaccggagg agtcggggct gcgggagccg gttcgtcggc
     6421 aatatttacg ccgaaatgct ctgccagcgg tttaagtcca ccgttgaacc cctgcgcaac
     6481 gaagcggaat ttccagccat tattacgacg gtaaaattca cccagaatta aggctgcttc
     6541 ctgacggccg ctcagttcga cacagccgct caccaggccg gtagcccctt gctccacgtc
     6601 gatggaaaga ttacgaagac cggacaccgt ctgcccaccg tcacaggtca cggtgaacgc
     6661 aattttctgg acatcaggtt tcagccggtt gagcgcgaca gtaaaggttg agtactgacc
     6721 ttcactgacc aggctgacag taccatcatc gttacgtggc tgaccataaa acaccatgtc
     6781 cgcatccccc tgcaccttcc cggcggcata caggcgaaac gctgaagcat ccacctggac
     6841 gcccgaggtt atccggaccc gcaactcttg ggctggaacg ggggcattgc cccccggcat
     6901 caggttcatt agcgaataac cgcagaaacg atatcggagt gcatatcgtc aattgtgcga
     6961 ccgcgacagg catgaccaag ggcggtaaag tcccagttgc cgttattacg gcgcagcgaa
     7021 gaaataacga tcccggtatg tgagccctgc tcagtcagct tgtagcaagc cagctctttg
     7081 ccggtttgat cgaccacacg acagaacgca ttttcgacgt cattgaacga ctgcccacgg
     7141 aagctgttaa ccgtaaacgc caggtattca acgtttgcag gcagacggga gaggttgacc
     7201 ttaatcactt catcgtcgcc gtcaccctca ccagtcaggt tatcaccgct atgcacaaca
     7261 gctttgcagg tggattccag tttgcggaac cagatggtgt caattgtttt accggtgctg
     7321 tccattaata cacagccagc atcgagatcg attgagtcgt tacctccaaa caatcctcca
     7381 agaaaacctt ttttcttaac cggatcccaa ccgagaccga aatgaagctg gcttaatgca
     7441 ggtgattctt tgctgagtga taccgtctga ttcttaacta atgaaaccat gttttattcc
     7501 ttaaaatgct gataactgag acaatcaaaa gtatatttag taactggcga agaaaatcat
     7561 atcatgatga agttctctga caagggaaat ttggtagctt tctgtaaaaa cacaaagaga
     7621 aatccttttc atgatgtcct ttcatctaaa taccaattaa atatcaacac attaacaggt
     7681 aacacaaaaa cactgataca ctccataacc tcaggtgtta ttaatcatat tatgattgac
     7741 atgtagcata tacccccata aactcacttc tcgacaataa attttcttgc cgttccggat
     7801 aataaaaaaa tgaacaaaaa aatttggttt atggaaggtt tgtcctctca gagagatatt
     7861 attcaggggg ttaaattatt tgcacaaaaa aataattttg ccattaccgt ttttgcctcc
     7921 caccgtaacg aaagaaatga aatcctttcc gttgccgatt attctttgac tgaacctgaa
     7981 gatcctcaaa aacgtcttca gtttattcag gaaaccattc agacctacgg catccaccat
     8041 attcatactg gtcgtaacaa ccagtggttt gaagaacacc gaaccgccat tgaatcgacc
     8101 ggtgccaccc ttactaccgg tgcaacgggc atcgactggt taactctggc tgacgaaaaa
     8161 gttacttttg ctcagtttat ggagcaaaag gggctcccgg tcgtaccatc ctggcgggtg
     8221 aatacgctgg cagaattaaa gacacgcctc gcggctccgc cgttcactga cagtccggta
     8281 tgcgtgaagc cggtgacggg tatctacggc atgggattct ggcgctttga tgacagtgct
     8341 ttgcctatgg ccgtctttaa tcaccccgaa catcgtctgg tcagcccgca actgtatatt
     8401 gctgcagcgt cggcttctga gtcgtttaaa ccccttgttt tgatgccgta cctgcctggc
     8461 ccggaatttt ccgtcgatat cctcgcggat aagggcgaaa tactcgcagc cgtgggacgc
     8521 cgtaaggaag gggctatcca gtatctggta aaccaaggaa gcgcctggga actggcgtgt
     8581 gactgcgccc gggttatgaa ggccgacggg ctggtgaatg ttcagacgcg aaacgatgtg
     8641 aatggcaacc cggtgctgct tgaaaccaac atgcgtccgt caggcggggt gggttatacc
     8701 cttcacagcg gggttaacct tcccgggttg tttgctgctt ttaagctcgg tctgatgtct
     8761 gaagatgcgg tgatccagag cgccagaaat accttttctc cggttgcagt gagatccatc
     8821 acagatgtca tcgtttaccc agaatcactc tctaaccttc tgaattaagg cgtgtttatg
     8881 tctgataccc tcgaagatat gatttatcgc cgtaccctct catgcggtac gattcaggta
     8941 acccgcgacc agggtgaagt ttcgctcgat gacctgtttg atatcgctga aagacgtaac
     9001 ccgaaacgcg cctttctgtt tgtcagcaaa gtactcggca ggcacattcc cgtgccaccc
     9061 tcagtcatgc ggcaggccta cagacagctt gccagccagt tcccctcaac gctgacaggg
     9121 cccatactgt ttatcggtat ggcagaaacc gccgttgggc ttggggctgg tgtgtttgat
     9181 gaagtgcgcc accagcatcg tgaatctgtc tatctgacct ctacccgtca cccggttgat
     9241 ggcacattgc tttgcgagtt caaggaagaa cacagccacg ccaccgatca tctgatctat
     9301 ctgccagacg atgaagagaa aagacgtcgt gttacaaacg cgcgaactct ggttttgatt
     9361 gatgacgaag caaccaccgg taataccttt attaacctgc tttctgccct gcgtaatacc
     9421 ggcaagcttc aacatattga acaggttatc gctgttacgc ttacggactg gagcgaaaat
     9481 gccctgtcca ggcgcagcac tttacccgtc acttcggttt ctcttgtaag tggtcagtgg
     9541 ggatggactc cattaccaga tgcacctgtc ccggacatgc cgaaggtcaa cgtaacttca
     9601 cgtggaaaat gggacatcca gggaaaacag tcctggggtc gactggggat gctcgcacct
     9661 gcggccgatc tcggtcatga ggtatccttc cgcaaggggg agcgtgttct ggttctcggg
     9721 acaggggaat tcgtctggga gccgttcctg cttgctgaac ggctcgaagc tgccggagca
     9781 caggcatttt atggtgcgac cacccgctcc cctatcgccg ttggttatgc cattaagtcc
     9841 gcaatttcct ttacggataa ctacggcctg ggcatcccca attttgtcta taacgtcgcc
     9901 catcagcaat ttgaccgcat tcttgtgtgt actgagacac ctgcagaaag tattgacacg
     9961 cggcttctta aagcgctggc tgaggttgca cccgtcgtgg agattgttac ctatgaataa
    10021 acccgttgtt ctgagcgacc ttgacgacac gctctttcag acccgtcgta aaatggtaga
    10081 cgagctggcc ctggagccat tccgtaccgg tgccgttgat cgtactctga atccacgaag
    10141 ctttatgaca gaagaacagt ccatgttagt ggactggctc ctagagcagg ctgaactcat
    10201 tcccgttacc gcacggggaa ctgaagaaat cagccgcgta aggatccctt tccactcctg
    10261 ggcaatcacc acgcacgggg ccgtcattct cacgcctgaa ggcaaacccg acgaggagtg
    10321 gaaggcccat atgctcggcc agctaactcc ctaccaggaa aagctggcat cgatgcagcg
    10381 tctgatcact gaaatgatgg acgcaaaagg gatcaatgcc tgggcaagac tgaacttcga
    10441 atacggtgaa acggcggttt atatggtgat gaaacaccgc gacagcactc gccttgacga
    10501 actcaatgcc attgcagatg agatagaaac ggtgttttcg accgagggct tctacatcca
    10561 ccgcaacagt aacaacgtag cctggcttcc caccccggtt gagaaagggc tggccgtcag
    10621 ctggctgctt gaaaaacttc gggctgaacg cggagttttc cccgtaattg gtctgggcga
    10681 cagcctgagc gatcatcgtt ttatgaaact gtgcagctgg tttggcatcc cgcgtcagag
    10741 tcagtttgca gatgccattt cacagcgaat ttttggagaa aattaaaatg cagccacatg
    10801 aaacatttac cggctcatac cagcccggtg acgtggaatt tctgctaaag ccggttgtca
    10861 ttgaaatgac gccagttgat caaaaagaag agctgattca gtccgggaag aaacattatt
    10921 cggacatgct cagccaggag ccagcgccga cacaatggca tcttgatttg tttcaccggg
    10981 cgctggatcg gggcgcagag agacttgcaa aagaagtcac acagctcgct attgctctgg
    11041 ccgaacgctt cggtgatgag cccatagtac tggccagtct tgtcagggct ggcgtaccgc
    11101 tcggcgttat gctacaccag gctctgcgtg acatgggaaa aacctcatgg cattacggca
    11161 tcagcatcat ccgggatcgt ggaattgacg gtacagcact tgatatcatt gaagagcggc
    11221 atggtaccag cggcattgtc tttgttgacg gatggacagg taaaggcgca attaccggag
    11281 agcttgtacg cgcactgaga gatcgcccgg gctatcctga gcagccgcga ctggttgtcc
    11341 tggccgatcc ctgtggctgt tcctggcttg cagcaagtga tgatgactgg ctcattcctt
    11401 ttggcatcat gggggctccg gtgtcaggcc tgctctcccg gtcagtatgg tcctctgaag
    11461 gattacatgg gtgcatggtt tgcgagcatc tcagtgaatt cgaatgcagc cggatgcttg
    11521 tcgataccgt cgctcatttc cgtaagaagt taacaccgtc atccctcgct cctctgagct
    11581 ggaatacgga gtcaacccgg gtcttatggc agacaagtcg cgacgttatc gcgttcctgg
    11641 ccgatgaatt taaagtggac agcgtcaacc gtattaaacc cggtattgct gaagcaaccc
    11701 gggctgtatt acgtcgggta ccggaccatg tatttgtgcg ttcgattgac gacccggacg
    11761 ttgccttact tgtggggctt gctcgtgaaa agggaataat tgttacagaa atggggggaa
    11821 ccctcggcca gtatcgggct gtaaccatta tcaaaaaggt attctaatga aaaatcgtct
    11881 ttctccctgg aatcttggtg ccactctata catgcctgca acacgggaag atattgctga
    11941 tgccgttctg cacgagaaaa tcccgggttt gcgctctctg gtgatttgcc tggaggatgc
    12001 tgtcagtgaa gcagacatcc ccgtcgccct caaaaatcta gagcacctgc tgcacgaact
    12061 cagtaacagc atgcgtagcc tgggtaaaaa taactggccc ctgttgttca ttcgcccccg
    12121 gcatgccgaa atgggcaaat ggttaaaaga acattatgat ctttccgctg tcgacgggtt
    12181 tgtgttaccg aaatttaccc taagttcgtt agctgaatgg tgggatatca tgggcggaac
    12241 tcacctgagc atgatgccaa cgctggaaac agaaaacgtc tttgatgtga ttcagatgcg
    12301 cgagctggcc acccggctgt tggaacatcc ctgccacaag cgcattattg cgcttcgaat
    12361 cggcggcaac gatcttatga acgtcgtgtc gcttcgccgt ccccgagccc tgacgctgta
    12421 tgacagtccg atgggctacg tcatcaaaat gctcgtttca gtcttcagcc cgcgtgattt
    12481 tgccctgacc gcgcccgtat gtgagcatat tgacgatcat gccgttatgg ccagagagct
    12541 ggctcttgat atggcacatg gacttgttgg aaaaacggcc attcatccag atcagataga
    12601 ggtcattcaa aacgcgctga tggtcactca gggtgagtac tctgacgctc tgcggatcct
    12661 gaactctacc caggccgtgt ttaagtcgca gggggcgatg tgtgaacctg ccacacatcg
    12721 ccgctgggcg gctggcattc tggacagagc ccggttttac gggttacata acgagcaaag
    12781 cgctgatgga atcagattac ttactataac ccagcatcat taaggaaatc ataatggggt
    12841 tcaggttcag aggtacttcc gtttctttct gtagccgggg ggcctatgcc aatctggggt
    12901 tacccggtac cgagctgagt taccgtaccc ggtttgagct ggccgcgcct tccggaggtg
    12961 gaaacaggac agcaaccgtc ccggatctca gacagtcgct tgagcaggaa gcagttgaac
    13021 tcatgtcagc ggtgaccgca attcgtaaca ttcacgagtt gacgctggat ccaaaaacag
    13081 gcatcagttg ggcagagctg gaagcagtat acctgcatag cagaacatcg ctttttcagg
    13141 ttccggcacc agtgcgtcct gaaaaaccag aataccatgc attgtcggaa aatcctgcag
    13201 ggaacaaagg cattgttttt ctgggtaaat ggtttgaatc ggaatcagct aaagctgagc
    13261 gtcaagcagg gaatcttcgc cgatggcggc aagaactgat tgatgtgaag cgtgagaatc
    13321 cccttcgaca gcaccggtac cagcaacggc agacgaccag ggccgaacag tattcaaact
    13381 ggaagtgtga tgctgaagaa catgaaaaaa ggctcgccac gactcaggca gatgcccggc
    13441 agttgttccg ggcagacgca gcgtttttcg aatcatacct tgcgggtgtg ctttcagaaa
    13501 ctgaatggcc gcgtgaaacg cttgttgcat ttgaagtaaa gccggagata tcaacagtcc
    13561 tgccggatgt tggtttagct gagattgaag atttccctga taagatttac ggcgtcaatg
    13621 cccggggcat ggagctgacg caaaaagcca tacgcgaaaa ctatgcccgc cacagccatg
    13681 gctgcttgtt ccgactggtc ggtatcgtgt tacatacgct acctttcgac aaggtgatcg
    13741 catcaggatt tacacaacga gtaagcggaa cgactatctg gaggatgttt atatcctgtc
    13801 ctgcaaatgc actcgcagcc agaaggcata gaacatattg atccggttga agcgttaggc
    13861 tatcaccctg ttattcgaaa gatgagaaac acattcattt ttcagcctat tgaaccacta
    13921 accctttaag caaactaaat attacgatgc aattaaatac cagacaggcc agaattttta
    13981 agcttgccaa cttactgggc tccggcaagc ctgtttccgc taccgacatt ataacctatc
    14041 tggactgctc agagccgaca ttaacacggg ccctcaaaga gctacgaaag tcatattctg
    14101 cagaaatcaa atacagtaag gcaggtcatt cctatcatct cgttaatccg ggtcaactgg
    14161 ataaaaagac acttcgccgg atgaatgagg cgcttgcaca gaatgctgat ctgaaaaccg
    14221 gtgagtctac aggaaaggtc gtactcgata aggataaaaa aacagccgta tcattatcgc
    14281 tacgcatgcg aattttaaga aaaattgacc gtctggctgc actgagtgga tcgacccgaa
    14341 gtgaagctgt tgaaaaactg gcactgcatt ccgttgacga actgataaaa gaatacagcg
    14401 ctaaaaaatc ctgatgacaa atctcagctc ggggtgaata ccccggtttt ttcatatttc
    14461 aactctgtct tggcgtgacg tatttttgat ctattctcct cccgcaatgt cagcagctcc
    14521 ttacagccta caaaacctag cgtcttgaca tcctcttgtg ctacgcatca atctttgggc
    14581 gatttacact ccactcatgc aaatctgtga tgtatgatga ttttcagtaa gtgtaaactg
    14641 actgccgtat gtagccatta agccagtatt ggtaacgtag atgcccacct tttcacttat
    14701 ttggtggaca ccactttgtc taactcgtca gattctgacc aaacgtttca ggctgtacgc
    14761 ttactctgaa ggggggagaa tagtagcccg tgatagcatt gagctaactg acagccagat
    14821 cgcagcacta aagcgtaaag ccagtgatga cgaggcctgt ggcgaaagcg aaacggctca
    14881 tccgggatat ctgggttcgc aggacacgtt ctatgtaggc aacctgaaag gtgtcgggcg
    14941 tatttatcag cagacgttcg ttgatacgta ctcgaaagtg gctcactgta agctttacgt
    15001 caccaaaaca ccgattaccg cggctgactt actgaatgat cgtgtactgc cgttttatac
    15061 gtctcagggc ctgccgatgc tgagaatact gaccgacaga ggcacggagt actgtggcaa
    15121 agtagagcag catgattatc agctttatct ggcaataaac gatatagaac acacgaaaac
    15181 aaaggcgatg tccccgcaga ccaacggcat ctgcgagcgg ttccataaaa caatcctgaa
    15241 cgaattttat caggtaacgt tccgtaaaaa actgtacggc gatcctgata cattacagtc
    15301 ggatcttgat gaatggctgg ctcactataa taatgagcga agccatcaga gaaaaatgtg
    15361 caatgggcgg acgccaatgg aaacgttact tgatggaaag cgcatctggg ccgagaaaaa
    15421 tttaagccag atgtaatctg acagatacct gtataaataa ccggtaactg tcagatcagg
    15481 tctgagctaa tacaaattaa ggttttaaat tcattaagta atcaatatca actggatgat
    15541 tataatccac tgactcaaat ccaaaaccat ttaattgtaa aaattctttt ttgtatccaa
    15601 tataatcacc tacagaagta aagttttcat cattcatttt cgacatcaaa tcaattactt
    15661 tttgttgtac atcctctctt aattcaagat cgtctatgcg aattagtctt gatgaatcaa
    15721 caacaacatt atcgacattg tataaatatt cagtaaaaag acgatgcatt tgtccaatac
    15781 atgtttcatg caatcccata gacttcatta ccttatataa gcagagtata taaggagaga
    15841 aacagggaat atacacacta gccttcgtaa ctaaagcttt acaaactact acataagcgc
    15901 cactatgttt atctgtggaa attaatttat ttagacgatc agatgtttcg tgtaaatgtt
    15961 gtttggcttt tcctaatgtc cctgtatgat aaatcggata agtcatttct ggaccgatat
    16021 atgaaaaagc aattgttttg gtcttcttcg taagaacatt attttccttt aaccaaagta
    16081 tccatgactc ccagtcatcc ccccccataa cttttatagt atctgcaatt tcagcctctg
    16141 aagcaacatc aataattgtt gattcaagtt tattatcttc gatattaacc gttttccctg
    16201 taaaagcact tccagtcgtt tttaaacagg aacgccaaat gttacctgtt gctggatctg
    16261 acctttgccc tgtggccaga ctatatataa taagatcgac ctcgccatca aaatccttat
    16321 ttattaagtt aagaacttta ttttttacat catcagagaa agcatcgcca ataacattat
    16381 gagcaattct gccttcagct tcagcaaatt ccttaaagaa tatattatta taccatcctg
    16441 cagttcctat agatttctca gagggacctt tttcaaacga aactccgata gaatcgcact
    16501 ttgcgccgcc aaaagtgaga gaaatccttg cagcaagacc aaatcctgaa gatgccccga
    16561 gaatcagaac tcgtttcggg ccattattaa tttgctgaag atgggactgg caatacttaa
    16621 tctgttccat tatcgcatga cgacatccct ctggatgagc acttttcgag acaacacctt
    16681 taattacagg ttttataatc atataaggtc cttgatagtt aaataaatta agatt
//
LOCUS       DAZGTJ010000103        16601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000103 DAZGTJ010000000
VERSION     DAZGTJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.103"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..351
                     /locus_tag="ACHK8E_004188"
     CDS             <1..351
                     /locus_tag="ACHK8E_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP0346383.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
     gene            719..1543
                     /gene="gadX"
                     /locus_tag="ACHK8E_004189"
     CDS             719..1543
                     /gene="gadX"
                     /locus_tag="ACHK8E_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP0346384.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1912..2640
                     /gene="gadW"
                     /locus_tag="ACHK8E_004190"
     CDS             1912..2640
                     /gene="gadW"
                     /locus_tag="ACHK8E_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP0346385.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLAADFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(3003..6116)
                     /gene="mdtF"
                     /locus_tag="ACHK8E_004191"
     CDS             complement(3003..6116)
                     /gene="mdtF"
                     /locus_tag="ACHK8E_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP0346386.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6141..7250)
                     /gene="mdtE"
                     /locus_tag="ACHK8E_004192"
     CDS             complement(6141..7250)
                     /gene="mdtE"
                     /locus_tag="ACHK8E_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP0346387.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7358..7636
                     /locus_tag="ACHK8E_004193"
     CDS             7358..7636
                     /locus_tag="ACHK8E_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346388.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7637..8164)
                     /gene="gadE"
                     /locus_tag="ACHK8E_004194"
     CDS             complement(7637..8164)
                     /gene="gadE"
                     /locus_tag="ACHK8E_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0346389.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNNPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8963..9535)
                     /gene="hdeD"
                     /locus_tag="ACHK8E_004195"
     CDS             complement(8963..9535)
                     /gene="hdeD"
                     /locus_tag="ACHK8E_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP0346390.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            9790..10122
                     /gene="hdeA"
                     /locus_tag="ACHK8E_004196"
     CDS             9790..10122
                     /gene="hdeA"
                     /locus_tag="ACHK8E_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP0346391.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            10238..10564
                     /gene="hdeB"
                     /locus_tag="ACHK8E_004197"
     CDS             10238..10564
                     /gene="hdeB"
                     /locus_tag="ACHK8E_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP0346392.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10628..11275
                     /gene="yhiD"
                     /locus_tag="ACHK8E_004198"
     CDS             10628..11275
                     /gene="yhiD"
                     /locus_tag="ACHK8E_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP0346393.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11327..12097)
                     /locus_tag="ACHK8E_004199"
     CDS             complement(11327..12097)
                     /locus_tag="ACHK8E_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFP0346394.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(12094..13086)
                     /gene="shuU"
                     /locus_tag="ACHK8E_004200"
                     /gene_synonym="chuU"
     CDS             complement(12094..13086)
                     /gene="shuU"
                     /locus_tag="ACHK8E_004200"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP0346395.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(13135..13758)
                     /gene="chuY"
                     /locus_tag="ACHK8E_004201"
     CDS             complement(13135..13758)
                     /gene="chuY"
                     /locus_tag="ACHK8E_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP0346396.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13758..14252)
                     /gene="hutX"
                     /locus_tag="ACHK8E_004202"
     CDS             complement(13758..14252)
                     /gene="hutX"
                     /locus_tag="ACHK8E_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP0346397.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(14265..15602)
                     /gene="hutW"
                     /locus_tag="ACHK8E_004203"
     CDS             complement(14265..15602)
                     /gene="hutW"
                     /locus_tag="ACHK8E_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP0346398.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(15622..16536)
                     /gene="shuT"
                     /locus_tag="ACHK8E_004204"
     CDS             complement(15622..16536)
                     /gene="shuT"
                     /locus_tag="ACHK8E_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFP0346399.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         4362 a         3927 c         3969 g         4343 t
ORIGIN      
        1 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg taccggtcgc
       61 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccaggatac
      121 accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
      181 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgccgtcg cggcttcgaa
      241 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgac
      301 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacatcac
      361 gttgtaaaaa ccgaatgccc aacctttaaa aacaggtggg tattcggcta cttattaatt
      421 gcaataatat attacctgtc tattcatcgt gttgataaga tatatgttat atgaatgtta
      481 tggagcatag cgatactgct taatattacg atgatgaatt atttgcttac tattaattcc
      541 tctgcgttct taagtcccct ttttatataa taaatccagt catccagccc gagtatcatc
      601 gctcattatc atcctgtttt cccgcttcgt ttaaatttat ttatcaatca atttgactta
      661 agagggcggc gtgctacatt aataaacagt aatatgttta tgtaatatta agtcaactat
      721 gcaaccacta catggtaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
      781 ggttaatggt gaatatcgct attttaatgg cggtgatctg gtttttgcgg atgcaagcca
      841 aattcaagtg gataagtgtg ttgagaattt cgtattagtt tcaagagaca cgctttcatt
      901 atttctcccg atgctcaagg aggaggcatt aaaacttcat gtacataaaa aagttccttc
      961 attactcgtt catcactgta ccagagatat tcctgttttt caggaagttg cgcaactatc
     1021 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agggcattaa tctttgcgtt
     1081 gttatctgtt tttcttgagg atacgcagtt tataccgtta cttctgaacg tgttgcaacc
     1141 gaatatgcga acacgagttt gtacggtcat caataataat atcgcccatg agtggacatt
     1201 agcccgaatt gctagcgaat tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
     1261 agaaggtacg tcatattcac agttgcttac tgagtgtagg atgcagcgtg ctttacaact
     1321 tattgttata tatggggttt caattaagcg agttgcagta tcctgtggat atcacagcgt
     1381 gtcgtatttc atttacgttt ttcgaaatta ttatgggatg acgcccacag agtatcagga
     1441 gcgatcggcg caggaattac cgaactgtgg ctcggcggca agtatggctg cgcaagggat
     1501 tttttacggc actgaccgtt ctgcggaagg aataagatta tagagtttta ctcagacata
     1561 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
     1621 cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact
     1681 tttactggaa ataagatcag ccattttttt ataaacataa gctatacgct gtgtgaaaat
     1741 ataatcgtgt tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata
     1801 aaatttggtc ctaccaaata tggcagtttt tgcacacaga aacagtctgg cgtcatttca
     1861 ttagtatact gacattgaaa taatcgcagt aatgaaatat aagggatagt catggctcat
     1921 gtctgctcgg tgatcctcgt tcgtcgttca ttcgatattc atcatgaaca gcaaaaaata
     1981 tcgttgcata acgagagtat cctgctgctg gataaaaatt tggcagccga ttttgcgttt
     2041 tgttcactgg atacgcgacg gctggatatc gaagagctga cagtttgcca ttacttacaa
     2101 aatattcgtc agttgccacg caatttagga ttgcatagca aagaccgttt gttaattaac
     2161 cagtcacccc ccatacagct ggtgacggcg atttttgata gtttcaatga cccccgggtc
     2221 aattcgccga tactgagcaa aatgctctat ctttcctgtt tatcaatgtt ttctcataag
     2281 aaagaactga tccccttact tttcaatagt atcagtactg tttcaggaaa agttgaacgc
     2341 cttattagct ttgatatcgc taaacgttgg tatctacgcg atatcgcaga aagaatgtac
     2401 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
     2461 ttactcgcct ccaggatgtc gatggccaga cgattactcg agttacgtca aatacctctg
     2521 catactattg cggaaaaatg tggctatagc agtacgtcat actttataaa tacatttaga
     2581 caatattatg gtgtaacgcc acatcagttt tcgcaacatt cgccgggtac cttttcctga
     2641 catatttcgc atttgaatat tggtcaggat ctcacgtctg cttcatgtga aactctttcc
     2701 tgatgatttc tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt
     2761 attttcagga ggaaaagact cggcatgttt gggattatta agctaacaat tcatacgatt
     2821 acgggaatgt gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc
     2881 tactttcagt gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt
     2941 gaatggttag caggaaagag taaggctgaa ccctcacgtt caaccttact ctcatttaca
     3001 cgttacgctt ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg
     3061 aagtaaattg ccagcactgt tgcggcaaac atcccgccca ttacgccggt acctaccgcg
     3121 ttttgcgcgc cagagccggc accatgactg ataaccagcg gcagcacgcc gagaataaag
     3181 gccagagaag tcatcaggat tgggcgtaaa cgcatacgcg ctgcttcgat gattgcctct
     3241 accggcgttt tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg
     3301 ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct ggaagtaaac gtcattactt
     3361 aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc
     3421 accgagaacg gaattgacca gctttcataa agtgcggcga gggcgaggaa caccacgacc
     3481 agtgaaatgg catatagcgc gggagcctgg tttgaggata acgcttcctg atacgacagc
     3541 ccggtccatg agtagccgac gcctgccgga agttttgcga ccaggtctgc cataaatttc
     3601 atggcgtcac cggtactttt cccggcagcc gcttcaccta aaatctccat tgacgggatg
     3661 ccgttgtagc gttccagtcg cggtgaacca taggtccatt cggtagacga ataggcagaa
     3721 agcggtgcca tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac
     3781 atacggaacg gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag
     3841 tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc
     3901 acacccatag cctcggcttt cgcagcgttg acgttcactt tgaacatcgg cgtatcttcc
     3961 aggccgttcg ggcgtacccc gatgacctga ttcggtgatt gcgctgccag tgataacagc
     4021 tcgtttcgcg cctgggtcag cttttcgtgc cccagattgc cgttgtccag cagttccata
     4081 tcaaaacctg acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg
     4141 gctttattga tactgcttag cgcaatcatt gcccgctgaa taatcgcggt aaccgagttt
     4201 tcctcgccga cacgttcaga ccacggcttg aggctgataa acgccagacc gttgttttgc
     4261 ccctgaccac tgaagccaaa gccaccaacg gtaaacaccg actgaacatt atttttctct
     4321 tttgtcagat aataatccgt cacctgttgt agcactttcg tggtgttaac catggttgca
     4381 ccggaaggta actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag
     4441 gtcggcgtac gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg
     4501 tagcgaccgg tacaacgcaa cagcgagcgg gtgctgtcgg tatagtgctg agttgatttt
     4561 tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt tgtgatcgcc ctccggcgcg
     4621 gctttcagaa tggtggcgca cagggcaggg gtcaggctca ttgctacaaa tactgaaagc
     4681 agcatggagg agatcagcgt gatggagaac tggcggtaga tctccccggt cgcaccgctc
     4741 ataaaggcca tcggcataaa cactgcggaa agaacaacgg caatcccgac cagcgcacgt
     4801 tggatctgcc ccatcgattt atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg
     4861 acacgctcga cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc
     4921 accatcccga acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac
     4981 gtcccgagaa taaccaccgg tacggcaatc gtcgggatga ttgtggcgcg gaaattctgc
     5041 aaaaacagat acatgaccag gaagacgagg atgatagcct caaccagcgt tttgaaaact
     5101 tcctgaatag aaattttgat aaacggtgtg gtgtcgtaag gataaaccgt cttcaggctt
     5161 gcagggaaat aagccgataa acggttcagc tcctctttca ccgctcgcga ggtatccagg
     5221 gcgtttgctc cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc
     5281 gccacggtgg aataatcttc cgccccaagt tcgacgcggg cgacatcacg cagcagtact
     5341 tgcgaaccat cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga
     5401 cgcgtctgca caatgatcga ggcgttcagt tgctggtctg ccgcctgtgg catgccaccc
     5461 agttgaccac cggaaatctg gttattttgc accttaatct gggaaataac atcggaaggt
     5521 accaggttat atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa
     5581 ccaaagagct gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac
     5641 gctacatagt ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaatgccgct
     5701 accatcagga tattactgct cgacttatcg acgctaatcc cctgctgctg caccgcttca
     5761 ggtaatgaag gcatagcgag ctgcagttta ttttgcacct gaacctgtgc gatatcagga
     5821 gatgtcccag tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa
     5881 gacatgtaca tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag
     5941 tcttctaccg tttgcgcatc ggcacccgga taggttgcgc tgacggtaat ggtcggtggc
     6001 gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc
     6061 ataataatgg caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa
     6121 gtccccttaa gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag
     6181 gaaattgctc gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga
     6241 tcgccagcct gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca
     6301 atttcgcgta gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg
     6361 gcgttgtggg tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc
     6421 actaatgccg tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc
     6481 cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg
     6541 gtctggctgt aacgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc
     6601 tgtttgattt gcccactggc gacctcttct ttcatgcgca agaaatcctg caccgactgc
     6661 gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta
     6721 gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag
     6781 gtgacatttg cgtattgcag gttgatagtc gcctgttcaa cagccgcttt ggcgacggtg
     6841 acattggctt ctgcttcatt caactgggtg cgcgcggtgt cgtaatcctg acggctaacg
     6901 tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta
     6961 gagagcgctt tcgccagcga gcctttggcg gagttcagct ctgcctgtaa aggcgcagga
     7021 tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta
     7081 atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctacca
     7141 ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc
     7201 gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag
     7261 aataacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga
     7321 gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg
     7381 catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt
     7441 caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa
     7501 gggttctact ggtggataca tataccaggg gaataaagtc gttttcgcct ctcctttagt
     7561 aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca
     7621 gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac
     7681 gtcttagttt cagtttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa
     7741 tattgagcgt cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt
     7801 tgaactgatt atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag
     7861 taatctcaag aagatttttg atactttctt tgcggctgac aaaataaata ggcgctttag
     7921 cttttagttt actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct
     7981 ccagaaattt aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct
     8041 tgaaggggtt attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt
     8101 gattatcttt caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa
     8161 tcataacttg ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat
     8221 ccttgtccga atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac
     8281 taatacgtct attaacacta atcaatactt cagatttatc catcacgcac tccatatatt
     8341 ggtcctaagt aagattcctg gttgttatca gtttgtaact agtaaacaaa aggttaactt
     8401 ttgcggatta ctgcccaaga ataagttatc aataaccagt ttgtgatctc tgaagaatat
     8461 tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct ccttgtttcg acttaggctg
     8521 gcaattggat tgccagcttt cttttaacgc attgaaatta cctgattaaa taactcattc
     8581 atgtcttcag gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg
     8641 ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac
     8701 ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac cacggaaaat
     8761 atcagccatg ataatgtttg aaatggctaa tttgccatgg aacgaaaaaa atcagattaa
     8821 attcagtagg ttgaaataat caacagcagg caattgtttc aatgatagtg cgtaattgaa
     8881 ctgaaacact gtatagctaa gagagtatta actggatcat agtcacttaa tgtgactatg
     8941 atccaggtgg aaatagggat aattattgct ttttaacgaa caaactggcg aagctgaaca
     9001 gactggcggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttaccgaca
     9061 ccatcggcgt agcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga
     9121 gctgtagcca actgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc
     9181 ctgccacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctctggtg
     9241 cgcggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata
     9301 ccggccagaa attatgactg cggttgctga atagcccgac aataagcgca atgcccgagc
     9361 agattaataa tgcacccact actgtgctta aaatgtcgcc agagacgaac ggaaaactga
     9421 tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgtt
     9481 ttttaagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaac
     9541 caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtgtaa
     9601 gaatgcagtc gatttaataa aaatttccta attacagtat ctgatgcgtc tgtaactcat
     9661 tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa
     9721 ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt tatagttgag
     9781 gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagta
     9841 gtgagcaatg cagcggatgc acaaaaagca gctgataaca aaaaaccggt caactcctgg
     9901 acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct
     9961 gaagcactga acaacaaaga taaaccagaa gatgcggttt tagatgttca gggtattgca
    10021 accgtaaccc cagctatcgt tcaggcttgt actcaggata aaaaagccag ctttaaagat
    10081 aaagttaaag gcgaatggga caaaattaag aaagatatgt aattccggga atgcgttaca
    10141 tcgtatttcc ttgcatattg aacaggccgg aatatcttct ttaaaagcag ctattcctcc
    10201 tgtttgtata taatctctat attgaatggg ttacaaaatg aacatttcat ctctccgtaa
    10261 agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct
    10321 ggcagccaat gaatccgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa
    10381 agcaatgaca ccagttgcgt ggtggatgct gcatgaagaa acagtatata aaggtggcga
    10441 taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgcaa
    10501 gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa
    10561 ttaatgaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca
    10621 tttttatatg acagcagaat ttattatacg acttatactt gcggtaattg cctgtggcgc
    10681 tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac atgtattaat
    10741 tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc
    10801 tttaggtcac gtcggactgg accccagccg tattgctgct caggttgtga cgggcgtcgg
    10861 gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc tgacgacggc
    10921 agcggatatc tgggtgaccg ccgccattgg tatggttatt ggcagcggta tgtacgaact
    10981 gggtatttat ggttcagtga tgaccttgct ggtgctggaa gtcttccatc aattaacctt
    11041 ccgcctgatg aacaaaaatt accatttaca gctgacatta gtgaacggta atacggtatc
    11101 aatgctcgac tggtttaagc agcaaaaaat aaaaacggat ttagtttcgt tgcaggaaaa
    11161 tgaagatcat gaagtggtcg ccattgatat ccagcttcat gccacgacgt cgatagaaga
    11221 tctgttgcga ttattaaaag gtatggcagg ggtgaaaggc gtttcaatta gctaatctgt
    11281 atagttattt attcgcagga gtaccagtgc ggtactcctg ttggttttaa tgatcgagga
    11341 cgatcagggg tgaatggttg gcaggatctt taagtacggt aatatccgcg ccgtaaagca
    11401 tggtcagcgc ctgctgtgtc aatacgtcct gtggtttacc atttgcgata acctttcctt
    11461 tttgcattaa tacaatacgg tctgcataac gcgcggcaag atttagatcg tgcaatacgc
    11521 agcaaacatt gaactgccgt tcgtgaacta actggcgtaa taaacggaac aagtgctgct
    11581 gatggtgaat atccagcgcc gaagttggtt catcaagaaa taaccatttc ggtgagggag
    11641 ttggttccca gagttgcacc agcaggcgcg ctaattgtac gcgctgttgt tcaccaccgg
    11701 ataattgccg atagtcgcgg tttgctaacg cctgacagtc acataacgcc attatttgtg
    11761 ctgtttcatt atcctgatta ccagtgcgat gcggatgccg ccccatctgg ataacttctt
    11821 gtacgctgaa tggaaacgcc atgtggctat tttgacgcat cacggcccgg tgtttcgcca
    11881 gttcagtaat cgaccattca tttaatggct tattaaacaa ccgacattcg ccgctatcag
    11941 gttgaagata gccggttaac tggcgaagca gcgtagattt tcccgcacca ttagggccga
    12001 gaatggcaac aatttcccca ccgggaaaag tcagcgagac attatcagtc agacgccgcc
    12061 cctgaaggct ataaaccaga ttctgggcgc tgatcatgtg atcctccggt tgcgcagaat
    12121 caaccacata aaataaggac caccaagcag gctggttaat aatcccacgg gcatttctgc
    12181 tggctgtacc agcgtgcggg caagcgtgtc tgccatcagc aataaacagg ctccggcgag
    12241 ggcggagcaa gggattagcc agcggtgatt tgctccggta gtcatgcgaa tcagatgcgg
    12301 gatcaccagg ccaataaagc cgataatgcc gcttaccgat acggcggcac caacgagcag
    12361 tgagctcact aacagtaatt gctggcgttt acgcttaacg ttcacgccga ggtaatgggc
    12421 ttcttcatca ccgagctgca gtaaattcag cgttccggcg agacaagttg ttgcgataat
    12481 ggccggtagg atgaatgaac tggcaaccaa taacgtcggc cattgcgcct gtccaagatt
    12541 gcccatcatc cacaatgtga gttgtcgtag ctgctgctca tcgccgatat agctcaggat
    12601 gccgatcgct gcgccgcaca gggcattgat ggcgataccg gcgagtaata attgcatcat
    12661 gctgccatcg cgatggcgct gcgtgatgag aaaaatgatg gtgcagacca ctaaacttcc
    12721 ggcaatggca aacaccattt gctcatagag caccagcacc actgggaagg agaagggcag
    12781 cacaatagca acgccaacca ttaatgcaga accgctactg acgccaagta atccagggtc
    12841 agccattggg ttgcgaaaaa gcccctgcat aattaccccg gcggttgcca acgctgcacc
    12901 taccagcacc gccagcaaaa cccgtggtag acgaatattg agccagatat cgcggtattc
    12961 ctcatcaaaa agggcatcaa aactgatctt taacgctccc tgactggcac caaacagcac
    13021 cagagctagc aataatagtg atagccccat aaagaccctg gcggaagaga gtgaatcctt
    13081 gagcacgcaa aactccgtta cgacattacg gcgcggacat gccgcgccgg gggatcacgc
    13141 cggtttcagg tccggttcaa tcagactgta gacctgctgg ttaagtgctg gcgcatttgc
    13201 cagttcatgg atatgtgcgg cgacatcagc acggttaata aaaccatgac actcctgatt
    13261 ttgtatccgt tgtgctttgc ccgttgccgc accatcaagc agaccgccag ggcggagaat
    13321 ggcataatca agctggctgg tttgcaacca gctttccgcc agcgtttttt cccgtaccgc
    13381 ctgaccaaac gcagcctttg cccgctctga taaaaacggc cagctatcgc cgcatcccag
    13441 tgacgtcacc agaatcatgc gtgtgatgcc tgctttctcg gcctcgtcaa tcactgttct
    13501 gtgcgccaga taatcctgag ctccgcccat tgttgagatg ataagcgcat ccgggccagc
    13561 tgcgcggcaa gcagccgcga ctacgctggc atcacaggcg tcgcctgtaa aaacctgtac
    13621 gccttgttgc gccagtttcg tggcggcatc ggcatgacga atgacagcaa cgaccggacg
    13681 ttgttccgcc agcgccagtt caagcgttct ggcacccaca ccttttccgc ctgcgccaaa
    13741 gagtagccac ggtgtcatca ggcgtgttcc tttaacgatg ctgccagagc atggaaggca
    13801 ctgacttgtt cgctcaggag ctgacgatga tcatccctac cgaggaagat tttcagcatt
    13861 gcgctgcctt ccttattgaa aaagagaata gaggcggtat ccatgcccat aaatttacgt
    13921 tcgatcaagg caatatgcgt gcagttttct gctttgatat gcccggacat gccgtgcttt
    13981 ccgcgtaggt tgaaatagcc gtggcgatgg aaacccgaag gcagttcgcc gctaaattcg
    14041 agtattacat cggtagtatg tactaacgtg gtgacgttgc cccattcgca gacggtatcc
    14101 cagacagtat cgaatttatc acctgacaca acagtcggag agggcagatt tttcactacc
    14161 tccagcagcg tggtgttgta ttgttcggct atcgcctcca gcgtaccgtc tggctccgtt
    14221 ttcaaaaatt cctgtaacga gacatggctc ataaatcact cctgttatgg tttttcaagc
    14281 gcaattgctg gcgcattaag tacctgaatc agttcattaa gagactgcaa aatgttgctt
    14341 gcccagaaac gaccttcatt ggtcaaccgc agacacgttg acgcatcgcg gcttaagcct
    14401 ttttggtgcc actgggccag taacggcgcg agtttttcgg catgtggcgt aagttcgtcc
    14461 agcggtacac gcgccgtttc aatgcccgac tgcaaggtat gacgccactg cgcgttacgt
    14521 tcggcgttac gcatgatcat catcagcggt tttttacctg ctgcgacgga ttcatgccag
    14581 gtctgaagat tgcgttcgtt catccaggag taaccattaa tcgaaccgcc ggctccggaa
    14641 ccaaaggcca gacaatcggc accttgttta atcagcaggt tatagagatt gcgttcgcgt
    14701 gtggtacggc cccagtggct gttactgatg cagcgccaac cggcatcgtc cataaaatca
    14761 cacccttgca ggtaaagatc gcgacgttct gccggagagg gcacggtagt acgcccattt
    14821 tccacggctt tgcccagcgg tgtattgggc aggacattga gcgcatagag atcgacgccg
    14881 tcgagaccga tatcgcgggc aatagccaga tcttctcccc aggtttgcgc atcctgaccc
    14941 ggcagaccaa acagcaggtc acagaccacc gcggcacggt cgcgtttgac caggctttcc
    15001 ataaacgcaa tagcggttgg gccatccgag gtgcgggcca ttttcttgcg gattttgctg
    15061 ttaaacgact gaataccaat tgagaagcgg tttgctccag catcaaggca agcatcgatt
    15121 cgctcagcgt caaaattcag tacccggcct tcaatagtga tttcacaatc aggggccagt
    15181 ggcagctttt ctcgtaatgt ggtgatgatt ctggccaaat cgtgtgctga aagcgcggaa
    15241 ggcgtaccgc caccgaaata gaccgcgtgg atgggggcag actgatgcaa tacgctgtct
    15301 gcttccattt ctatttcacg aatcagggcg tcggtataat gagcacacgc atcgtcatta
    15361 aaacggttct gataaaaacc acaaaacgtg cagtgtgtcg cacaaaaagg aatgtgtaga
    15421 taaaccagtc gcttacgtgg tgataccgtt tgattgagca tctcttgcca ggtctgtgtc
    15481 agttgctctt tggcaactgg aatggcccca cgaaacggca tcatggcgcg cctgtcttta
    15541 aatggctgat caccgtctaa agcaaaatgt ggcgtgagat ccagggtgtt atttgtgttc
    15601 attcgtctga tttaaatcca tttattgcgg ccaaagctgc tggtgtaaag actcaacgac
    15661 atcagcaata cgcggaccca ttcccagaat caggttttga tcaacggtga taatgcgctg
    15721 gtttttccat gcggcagtgt gggtaattcc ggcaatagaa cgtagccgat taatatcgcc
    15781 atcgaccatt tgtgaggtga cgacaatcac ttcagggtta gccgcaatca gcgattccgc
    15841 gctgtagctt ttgtactgct ggtgggtagc gacgttttct gctccggcaa gcgaaagaat
    15901 ggcgtccgcg acgctgcctt taccggcaac ctgtggcgca cttccgcctg cggagagaat
    15961 aaacatggct ttaaccgggg cttttttggc cgccacgttt tgctgtactc gctccaggcg
    16021 ttggttgatc tgtgtcacca gcgcctcgcc ttgttcagga acctgtaacg tttttgccag
    16081 ttggcgaatg ttggcgtaca tctgctcaag ggtggctggt acacgcggca gagtgacgac
    16141 gttgactttt tgcgcccgca gttggtcgag cacaatttgc ggtcctgcat cctgccaggt
    16201 aattacgcta tccgggcgaa gtgacaaaat gccttcgctg ctgagttgtt tccagtaacc
    16261 aatatgcggc agtttggcgg tttctggtgg ataagatgtc gtttcatcga caccaaccac
    16321 gcgttcgcca gcgcccatcg cgtagattag ctccgtcagc gatcctcctg cgaccacgat
    16381 acgttcggta gccgttacgc taaaagccca gctaagagcc agtactgccg taaacaattt
    16441 ttttcgtttt accgcggtct tcgcggcaga ggccacggct gaaatacata acgtcagtgg
    16501 ggaaaaaaga aacggcctgg tgatgatcct tggcataata tgagaactct tatcaaattg
    16561 agaatgatca tgattatcat ttgagttata tatgaaatac a
//
LOCUS       DAZGTJ010000104        16458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000104 DAZGTJ010000000
VERSION     DAZGTJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.104"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            61..1134
                     /locus_tag="ACHK8E_004205"
     CDS             61..1134
                     /locus_tag="ACHK8E_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0346400.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
     gene            1726..2577
                     /gene="hchA"
                     /locus_tag="ACHK8E_004206"
     CDS             1726..2577
                     /gene="hchA"
                     /locus_tag="ACHK8E_004206"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP0346401.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2684..4042)
                     /gene="hprS"
                     /locus_tag="ACHK8E_004207"
     CDS             complement(2684..4042)
                     /gene="hprS"
                     /locus_tag="ACHK8E_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP0346402.1"
                     /translation="MKKLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4042..4713)
                     /gene="hprR"
                     /locus_tag="ACHK8E_004208"
     CDS             complement(4042..4713)
                     /gene="hprR"
                     /locus_tag="ACHK8E_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP0346403.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4846..5259
                     /gene="hiuH"
                     /locus_tag="ACHK8E_004209"
     CDS             4846..5259
                     /gene="hiuH"
                     /locus_tag="ACHK8E_004209"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP0346404.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5368..6372
                     /gene="msrP"
                     /locus_tag="ACHK8E_004210"
     CDS             5368..6372
                     /gene="msrP"
                     /locus_tag="ACHK8E_004210"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP0346405.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            6373..7008
                     /gene="msrQ"
                     /locus_tag="ACHK8E_004211"
     CDS             6373..7008
                     /gene="msrQ"
                     /locus_tag="ACHK8E_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP0346406.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            7265..7915
                     /gene="zinT"
                     /locus_tag="ACHK8E_004212"
     CDS             7265..7915
                     /gene="zinT"
                     /locus_tag="ACHK8E_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP0346407.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(8585..8674)
                     /locus_tag="ACHK8E_004213"
     tRNA            complement(8585..8674)
                     /locus_tag="ACHK8E_004213"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8638..8640),aa:Ser,seq:cga)
     gene            8768..9565
                     /gene="mtfA"
                     /locus_tag="ACHK8E_004214"
     CDS             8768..9565
                     /gene="mtfA"
                     /locus_tag="ACHK8E_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP0346408.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9666..9741
                     /locus_tag="ACHK8E_004215"
     tRNA            9666..9741
                     /locus_tag="ACHK8E_004215"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9699..9701,aa:Asn,seq:gtt)
     gene            10054..10827
                     /locus_tag="ACHK8E_004216"
                     /pseudo
     CDS             10054..10827
                     /locus_tag="ACHK8E_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            10877..11812
                     /locus_tag="ACHK8E_004217"
     CDS             10877..11812
                     /locus_tag="ACHK8E_004217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFP0346409.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQTYEFFYGACYKQI"
     gene            complement(12074..13126)
                     /locus_tag="ACHK8E_004218"
     CDS             complement(12074..13126)
                     /locus_tag="ACHK8E_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP0346410.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKSASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPKYELPLSKDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEEKARVLVDCYNNVFRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLLRI"
     gene            13441..14757
                     /gene="shiA"
                     /locus_tag="ACHK8E_004219"
     CDS             13441..14757
                     /gene="shiA"
                     /locus_tag="ACHK8E_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP0346411.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            14859..16313
                     /gene="amn"
                     /locus_tag="ACHK8E_004220"
     CDS             14859..16313
                     /gene="amn"
                     /locus_tag="ACHK8E_004220"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP0346412.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4440 a         3780 c         3620 g         4618 t
ORIGIN      
        1 ttttggataa cggcacttat tgatatattc atgaagatta taatcacaag ggaatacata
       61 atgaaaagaa aagttctggc aatgctggtc ccggcgttat tagttgctgg cgcagcaaat
      121 gcggctgaaa tttataataa agatggcaat aaagttgatt tctacggcaa gatggttggc
      181 gaacgcatct ggtcaaatac tgatgataat aacagcgaaa acgaagatac ctcctatgct
      241 cgtttcggtg ttaaaggtga aacccaaatc accagcgaac tgactggttt tggtcagttt
      301 gaatacaacc tcgacgccag caagcccgaa ggctctaatc aggaaaaaac ccgtttaacc
      361 ttcgcaggtt tgaaatataa cgagttagga tctttcgact atggtcgtaa ctacggtgtt
      421 gcttatgatg ccgcagctta taccgatatg ttagttgaat ggggtggtga ttcctgggcg
      481 tccgctgaca acttcatgaa cggtcgtacc aacggtgttg caacctaccg taactctgat
      541 ttctttggtc tggttgatgg tctgaatttt gctgtgcaat atcaaggtaa gaacagcaat
      601 cgtgacgtta cgaaacaaaa cggtgatggc tatgcgttgt ctgtagacta caacatcgaa
      661 ggttttggtt ttgtaggtgc atatagcaaa tctgatcgta ctaatgaaca agctggtgac
      721 ggctacggtg ataacgctga agtgtggtca ttagcagcca agtatgatgc aaataatatc
      781 tatgcagcaa tgatgtacgg tgaaacccgc aacatgaccg ttttggctaa tgatcatttt
      841 gcaaataaaa cccaaaactt tgaagctgtt gtacagtatc agttcgactt cggtttacgt
      901 ccgtctttag gctacgtata ttccaaaggc aaagatcttt atgctcgtga tggacataaa
      961 ggtgttgatg ctgaccgcgt aaattatatc gaagttggta cctggtacta cttcaataag
     1021 aacatgaacg tctacacagc atacaaattt aacctgctgg ataaagacga tgcagcgatt
     1081 accgatgccg caactgatga ccagtttgca gttggtatcg tctaccagtt ctaatcagcc
     1141 aacgcgccgc ggcgtgttac tgtaaaactt aacaccagcc tgagttcgtc cctcatcgaa
     1201 ctcaggcttt tttattggca tttcatctcc atctgcggca cacgcaattg ccaaagcata
     1261 tcgatacagc accttacccc acccctcctg gctcaataaa atctaccagc gtctcttccg
     1321 gccaacatcc aaatgcattt ctttatgaat taatccgcaa caaaaccgcc gtccatatag
     1381 aaatatttgc aaaaatagtg catgaaaaaa gctttttctc taccaatccg cgatataaat
     1441 attcatcagc accacaatat tccacagcac taaatctctc cctgccgccc cggacctctg
     1501 ataatggtct aaaatcattg aggccacttg cgacgacgta gctcaataag gaaaatttgg
     1561 cgtgaataat attcaacacc agtgcagcat catcattaca tacttatgcc cgccaactac
     1621 cgctttcgat atattgactc aatttttcag cctggttaaa aatagcgacc agctcagtcg
     1681 caaatatagt gactacccta actaagcaac aataaggaat acactatgac tgttcaaaca
     1741 agtaaaaacc cgcaggtcga tattgctgaa gataatgcat tcttcccttc agaatattcg
     1801 cttagccaat ataccagtcc cgtctctgat cttgatggcg tggactatcc aaaaccgtat
     1861 cgcggtaaac ataaaattct ggtgatcgcc gcggacgaac gttatttgcc gaccgataac
     1921 gggaaactat tctcgaccgg taaccatccg attgaaacgt tgctgccgtt gtatcatctc
     1981 catgctgcag gtttcgaatt cgaagtggcg accatttccg gtctgatgac caagtttgaa
     2041 tactgggcta tgccacacaa agatgaaaaa gtaatgccat tctttgagca gcataaatcg
     2101 ttgttccgca atccgaagaa actcgcggat gttgtcgcaa gcctcaacgc tgatagcgaa
     2161 tatgcagcaa tctttgttcc tggtggtcat ggcgcactta ttggtttacc tgaaagccag
     2221 gacgtagctg ccgctttaca gtgggcaatc aaaaatgacc gttttgttat ctccctttgc
     2281 cacggcccgg cggcttttct ggcgcttcgc cacggcgata acccactgaa tggttattcc
     2341 atttgcgcat tcccagacgc cgcagacaaa caaacgccag agataggcta tatgccgggt
     2401 catctcacct ggtacttcgg cgaagagctg aagaaaatgg gcatgaacat cattaatgac
     2461 gacatcaccg ggcgagtaca taaggaccgt aaagttctca ccggcgacag tcctttcgca
     2521 gcgaatgcgt tgggtaaact ggcggcgcag gaaatgctgg cagcttacgc gggttaatcc
     2581 ctgttacatt gtaatgtaag cgcattatca atcgcagcca cgttaatact cacgacgtga
     2641 ctgcgttttt tcacggttaa ttttggcgta ctgaagccct atattaattt ctttgcggta
     2701 acgtaattcg gaacacgtta tgcttattga gatagtgata cgtagcactt cccccatgca
     2761 attcggcaat cgctttgact aaagaaagac caagtccctg acctacggaa tgacgcgaat
     2821 tatctccccg ccaaaatcta cggaagagtt tttcaggctc atgaattttc gttccaggac
     2881 tggcgatatc aatattaaga gagccgttgg tatcaagaaa actggttatc tgaatgcgcg
     2941 atttttctgg tgaatatcga atagcgttaa caataagatt cgataacatt cgttgcagta
     3001 aaattttatc cgcaaagatt tgctgattgc actcgacctt aaggcaaatc tctttctcgt
     3061 ctgaaaggta ttcaagatag tccaacaaat tttcgacttc cttattgaga gaaagcgaat
     3121 ccagtttcac caaaacattg tttttatctg ccctggcaag aaacagtatg ttctctgtta
     3181 accgcgaaat attctccagc tcttcaatgt ttccggcaat tgttttttga tattcagcga
     3241 tacttctggt ttgactgagc gtaacctgat tctgacccag taatgcatta attggtgttc
     3301 taagttcatg agcgagatcg tcagcaaact gacttagacg ctcaaaatct ttgactaaag
     3361 catgatgcat tttattcaac gcctgcccaa gaggttttag ttctctcggt aatgcgctaa
     3421 cctcaacagg ctcccggtta tcgttataat tcagcgcttc cgttacacca ctcaactttt
     3481 tgatctctcg taaccccgtt ctgattaaca gcggacttaa tactgagcaa agtacaatgg
     3541 cgacaatgca aattataatg ctattaattt tatactgttc aagcatgtta tgtctggctg
     3601 aagccaattt agccacagta accgttaatg atggcgaaac ttcatcaata ttaattcgta
     3661 aagcatctat ctctgtgtca ttaataatgc ttctgtaaat gccagctgcg ctgattgtct
     3721 cactagcagg tatgttattt aacatgccat cactgacatt tgtccggtta acaattttat
     3781 tgatgccatg accatgaatg atcaagatat cctgactaac atccatcatc cggttaaaat
     3841 agacaggtaa cgtatctgga tttaccccat caattaacaa ctgcttgatc tgcgctgtcc
     3901 ggttaatgag tgttgtatca tcgcgccatt tcaactcact tgccaggcca ttatagagag
     3961 tccatacaat tccagcgcca gcaacagaca gcaccaatat aaaaagcaag gttaaacgga
     4021 cggttataga taattttttc attatttttt taccgctacg aatgaatatc ccatcccacg
     4081 gattgtggca atgagctttt caggaaaagg atcatcaact tttgcgcgga gcctgcgaat
     4141 ggcgacatcc accgtattgg tatcactatc aaagttgatt ccccaaattt cactcgcaat
     4201 aaccgttctg ggtataattt cgccagctct ggaggccagt agccaaagta actgaaactc
     4261 cttgcgcgtc agtgtaatac tgatattgtt cctgctcaca ctttgactaa cagagtccat
     4321 tcttaagcca ctgatttcta atgttgaatt caaagcgtga tgttgcctta attgtgcccg
     4381 aacccttgcc agcaactcag aaaatgaaaa aggttttacc agataatcat ttgctccact
     4441 gtccagccct ctgactctgt catcgacaga atcccttgct gtaaggcaaa taacaggagt
     4501 ttgctttgcc gttcttaacg tttgtaagat ctgccagcca tccatacccg gaagcataat
     4561 atccagaatg atcaatgcat aatcatcctt cagcgcaaga taaagcccat ctctgccatc
     4621 agaaacggca tcaatgacat aacccgcttc ggaaagcccc tgcgttaccc actcctgggt
     4681 cctttgatta tcttcaataa gtaaaatctt catgaaaata atatgccata aatcatatgt
     4741 tatagaaaca gcctggttca ttacaaaatt gtaatgctgc tgtaaggtta ccctggacgc
     4801 tttttcgcta tcctcaaaac tcattcacat gacaaggata taaacatgtt aaagcgttat
     4861 ttagtactct ccgtagtaac ggctgcattt tcattacctt ctttggtcta tgctgcacaa
     4921 caaaacattc ttagcgtgca cattttgaac cagcaaacag gaaaacccgc tgccgacgtg
     4981 acagtcactc ttgaaaagaa agcggataac ggctggttac aacttaatac cgccaaaaca
     5041 gataaggatg gacgaattaa ggcactgtgg cccgagcaaa ctgcgactac gggcgattac
     5101 cgtgtcgtat ttaaaaccgg ggactatttc aagaaacaaa atcttgaaag tttcttccct
     5161 gagatccccg ttgaatttca tattaataaa gtgaacgagc attatcatgt gcctttatta
     5221 cttagccaat atgggtattc aacctatcgt ggcagttaat gcataaatat ataaaagcag
     5281 atctctgcta ctgttaggga ggtgactccc tgacagatta acagtaaacg gctcttgctg
     5341 gctaacgaca aaaaagtgtg atggcttatg aaaaagaatc aatttttaaa agaatcagat
     5401 gttacggccg agtcggtatt ctttatgaag cgtcggcagg tgttaaaagc actgggcatc
     5461 agcgcggctg cactatcttt gcctcacgct gcgcatgccg atctgcttag ctggtttaaa
     5521 gggaacgatc gtccacccgc ccccgccgga aaaccgctgg agttcagcaa gcctgccgcc
     5581 tggcaaaata acctgccact gacgccagca gataaagttt ccggttataa caacttctat
     5641 gaattcgggc tggataaagc cgatcccgcc gctaatgctg gtagcctgaa aaccgatcca
     5701 tggacactga aaatcagcgg cgaagtagca aaaccattaa ccctcgatca cgatgattta
     5761 acccgtcgct tcccgctgga agagcgtatt tatcgtatgc gctgcgtgga agcatggtcg
     5821 atggtggtgc cgtggattgg ttttccgctg cacaaattgc tggcgcttgc cgaacccacc
     5881 agcaatgcga agtatgtcgc tttcgaaaca atttatgcac cggaacagat gccaggccag
     5941 caggaccgct ttatcggcgg cgggctgaaa tatccttatg tcgaaggatt gcgtctcgac
     6001 gaagcaatgc atccgctcac actgatgacc gtaggtgttt atggcaaggc gttaccgcca
     6061 caaaatggcg cgccggtgcg actgattgtg ccgtggaaat atggctttaa agggattaaa
     6121 tcgatcgtca gtattaagct gacccacgag cgtccgccaa ccacctggaa tctggcagcg
     6181 cctgacgaat acggttttta cgccaacgtt aatccgcatg ttgatcaccc gcgctggtca
     6241 caggctaccg aacgatttat tggttcaggc ggcatcctcg atgtacagcg ccagccaacg
     6301 ctactgttta atggttacgc cgagcaggtg gcatcgctgt atcgtggcct ggatttgcgg
     6361 gagaatttct aaatgcgtct gacagcaaaa caggtgacat ggctgaaagt ttgcctgcat
     6421 cttgccggat tgttgccgtt tctctggctg gtctgggcga ttaatcacgg tggattgggt
     6481 gccgatccgg tgaaagatat tcagcatttt accggtcgca ctgcactgaa atttttgctt
     6541 gcggccttgt taatcacacc tctggcacgc tacgcaaaac agccgttatt gatacgcact
     6601 cgccgcctgt taggcttatg gtgctttgcc tgggcgacac tgcatttaac cagttacgca
     6661 ttactggagc tgggcgtgaa taatctggcg ttattaggta aggagttaat tacccggcct
     6721 tatttaacgt taggaattat cagctgggtt attttgcttg ctttagcgtt tacttcaacc
     6781 caggcgatgc agcgaaaact cggcaagcac tggcaacagt tgcataattt cgtctatctt
     6841 gtcgcgatcc tggccccaat acattatctg tggtcagtga agattatttc accgcagcca
     6901 ctcatctacg ctgggctggc ggtactgctt ttagccttac ggtataagaa gttgctttcc
     6961 cttttcaacc ggttacgcaa gcaggttcac aacaaattat ctctataact tgatattgcc
     7021 gacaaagtac gcgttatccc tgccggatgc ggcgcgagcg ccttatccgg catacacaga
     7081 tcgcattagg ttatgtttcg tttgtcatca acctcaaatc atcgacataa tttattttat
     7141 ttcaataaga tagataagca cacctgagag aactattctc ttgtctccta agagttgttg
     7201 cattttgtta tatgttacaa tataacatta cacatcatat acattaactc tggaggaaac
     7261 tgttttggcg attcgtcttc ataaactggc tgttgcttta ggtgtcttta ttgttagcgc
     7321 tcctgccttt tcgcatggtc atcactcaca cggcaaaccc ttaacagagg tcgaacaaaa
     7381 agctgctaat ggtgtttttg atgatgccaa tgtacaaaac cgaacgctaa gtgactggga
     7441 tggagtctgg caatccgttt atcctttatt gcaaagtggc aaacttgacc ccgtctttca
     7501 gaagaaagcg gatgcagata aaactaaaac atttgctgaa attaaagatt attatcacaa
     7561 aggttatgca acagatatcg agatgattgg cattgaggac ggcattgttg aattccatag
     7621 aaataatgaa acaacatcct gtaaatatga ttacgatgga tacaaaatac tcacctataa
     7681 atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa gatcctgaaa gcaaagcccc
     7741 taaatatata caatttagcg atcatataat tgcaccacga aaatcatctc attttcacat
     7801 ttttatgggt aatgattccc agcaatcttt attgaatgaa atggaaaact ggccaacgta
     7861 ttatccctat cagttgagta gcgaagaagt ggtcgaggag atgatgtctc attgattttt
     7921 ttgcgctgac ttcagttatt taacagaaca gtgcctggta gtaacaacaa taatttacca
     7981 agacaaataa gtaagacaaa ctcagacacg ctcatacttg agtcgtgtct tttttattat
     8041 gtctgtagaa aaaaactgcg aaaaaggcga acaataaata cacaactcag ccatacagac
     8101 tggcagcgca aatgtttcca ggagcaagtc atctgtcatg gtaaagctaa ccagaaatat
     8161 aggttaattt tggtgtttaa acctttttta cttgttagta tgatacaaag gctttcaaaa
     8221 aagctgggaa attcaacaac tcttggaaat tatggacttt gtcccgcggc tttaatctga
     8281 cggccgcgtt ccttttttgg ctcaattatt gaccccttct ctacgcaact tcagttcccg
     8341 ccagcaactt tgcggcagtt tcataggatc aatgtctaaa agaataatgg tgatcgataa
     8401 gaaaacgact gaataactgt agattttcgc tcgaaacctt cctgtcagct ccatagcgaa
     8461 tcaagtgctg aatgtcacag tatcgaacag aaaacagtga cgatctaacc cttcaagaat
     8521 attctacgat tgttctgttt aggaaaagca aggcgggaag tcgggagata agtcattgat
     8581 aaagtggcgg agagaggggg atttgaaccc ccggtagagt tgcccctact ccggttttcg
     8641 agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg
     8701 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
     8761 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
     8821 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acggaacagg aacaaagcaa
     8881 attagtcact cttgccgaac gttttttaca gcaaaagcgg cttgttcctt tacagggctt
     8941 tgaactggat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
     9001 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
     9061 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
     9121 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
     9181 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
     9241 cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca
     9301 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc
     9361 ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     9421 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     9481 ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     9541 gttttcggcg acgaatgttc attaattaac aactttgcag attaattaac caattgaaat
     9601 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     9661 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     9721 cgagtccagt cagaggagcc aaattctaaa aattcgcttt ttagcgcaat gtcactgacc
     9781 ttagttgagc attgcttttt aacggatagc gggtttttga catcttaagc gccctcgacc
     9841 tttatggttg agggcgtttt gctatgaacg ccatcaccat tttcccctcg attacaaaac
     9901 ttgagtaatt cagtagtctc ccctcttgca actcacaccc aaaactgctt aacgaaaagt
     9961 tattaatttt caatcatatt gctatcagga tttacatttt ttcgctgtgc tagaaagggc
    10021 gcatttatgt tagctcgttc agggaaggta agcatggcta cgaagaagag aagtggagaa
    10081 gaaataaatg accaacaaat cttatgcggg atgggtatta aactacgccg cttaactgcg
    10141 ggtatctgcc tggtaactca acttgtgttc cctatggctg cggcagcaca aggtgtggta
    10201 aacgccgcaa cccaacaacc agttcctgca caaatcgcca ttgcaaatgc caatacgatg
    10261 ccctataccc ttggagcgct ggaatcggcc caaagcgttg ccgaacgttt cggtatttcg
    10321 ttggctgagt tacgcaaact caaccagttt cgtacgtttg ctcgaggttt tgataatgtc
    10381 cgccagggtg atgaactgga tgtcccggca caagttagtg aaaaaaaatt aaccccgccg
    10441 ccgggtaata gcagtgacaa ccttgagcaa cagatagcca gtacttcaca gcaaatcggg
    10501 tctctgctcg ccgaagatat gaacagcgag caagcggcca atatggcgcg tggatgggcc
    10561 tcttctcagg catctggcgc attgacagac tggttaagcc gcttcggtac cgcaagaatc
    10621 acgctgggcg tggatgaaga ttttagcctg aagaattccc agttcgattt tctccatcca
    10681 tggtatgaaa cgcctgataa tctctttttc agccagcata ccctccaccg tactgacgaa
    10741 cgtacgcaga ttaacaacgg cttgggttgg cgtcatttca ctcccacatg gatgtcgggc
    10801 atcaacttct ttttcgacca cgatcttaag cgcagaaacg agctatggtg gggcgcaaac
    10861 agtagatata acgctggtgg caggcccggc agacgcctcg cagtccgtcc ttaaaaacaa
    10921 ccggtcatca ctgaaagggg actttaccga aagtgctgaa ctatatcttg ttttacacga
    10981 tctatcaggt catccgatca atgtttctga agggctggaa tttgtgcaat caggtaccaa
    11041 cgtgtcctat gtgcaaatca gcacaattga ttacactcaa aatttatacg gtgagtataa
    11101 agccactgtt acaggcggcg gagagggtat cgcaacgctg atccccgtgt tgaatggtgt
    11161 tcaccaggcg ggtctgagta ccacgataga attcattagc gcgggaacca gaccaatgaa
    11221 cggtacagta tcagtgaatg gtgcaaacct acctgcagct tcattccctt cgcagggatt
    11281 taccggggcg tattatcagt tgaataatga caactttgca ccaggaaaaa cggcggctga
    11341 ttatgcgttt tcaagcactg cctcctgggt tgatgttgat acttccggta aggtgacatt
    11401 caaaaatgtt ggagatagaa atgctgtaat aatcacggcg accccaagat caggtggcgc
    11461 tatttaccaa acgcaagtac gggtgaaggg ctggtgggtg aatcacggta ataacctcat
    11521 gcaattaagc caggctgaaa actactgcag caaccaggtc ggtaatggct acccccttcc
    11581 ccgcgcggac cttttaagca atgggcacat gcggcgcgaa atagggtcac tgtatggtga
    11641 gtggggagat atgggaaatt acatgaagga agctgatttt tattcaatgg tatattggtc
    11701 ttctaattcc gcaggcgcag gccaacaata cattgtatcc ctggaaacag ggacgcaaaa
    11761 cacttatcaa acgtatgaat ttttttatgg ggcgtgttat aaacagattt aatttttcgt
    11821 tataatttct tacagagtgc tccattcggg gcgctctttt ttacattctc tctacagaac
    11881 ttgcccttct ctcacagaaa atgaataaat aaaaatgcga taccgccaga ttcgcgttcg
    11941 gtgtgactat ggttgtatcc ggtgagtaca gctttgatcc aaaggctggc tttctgtaat
    12001 gattgaccag ccacaaagta ttgatgacac caccgttcat aacagatcgg cactactatg
    12061 cataagtact gcctcatatt ctcaacaaaa cactttttaa tgcctttgcg aattcatcca
    12121 gacttatatt gtcaattttt tgcccaacga cgactgactc cgagacatcg gccattgcgg
    12181 gccatcgcgt tttgtaatca gcataaattg caacaacggg ggtattatag gcacttgcca
    12241 tatgtaatat tgaggtgtca ggagttattg cgatataagc atcttttatg attgctgccg
    12301 aacgtttaat cgagggtgat aaggataaac ggaagacatt attataacaa tccactaaca
    12361 ccctggcttt ttcttcgcct tttggtccat gaacgatcac tattggtata tctgtttcac
    12421 tttgaatttt ttctattaga ttttctgcaa tcgataatga aaatgtacgt tcctgcgagc
    12481 taccttctaa attaagcgca atgtaagatc ctaacgatcg catttcctcg cgcacctcat
    12541 cgagtacatc cttacttagt ggcaattcat attttggcct gactgctgga aaacccgcct
    12601 cacgcatcat aaatgcccat gtcatgggta caggagcccg gagattctga tccatccggg
    12661 acgcgctctt acacaaaggg gaatagcact tcatggttaa accaacaacc tgaaaattcg
    12721 tttttgcttt caactgactg ataaatatca tcgttttcag gttcttttta cgcatcgcct
    12781 ctatacaaag atccggcgta ccgtattgct gtgttatgtt cttcgctaga tcttttattt
    12841 cttttaatgt tgcgtgatcc tgcatagtca ttgtaactaa tgttaattta gtctgttcaa
    12901 gtttgagcgc attaaagact tctaaattaa ttgtcgacgt tacaattaaa agatgcttaa
    12961 ttttatgcaa ttcaagcgcc cgaataacag gaaagatggc catagcatcg ccaatctgat
    13021 cgggaatatg gatgacaaca aagtctgttt tttcaatatt gaaattataa gctttataat
    13081 cgtagtaact aaatgcaata cgtctcaaca atgatgctaa aaacatacct aacctcgcct
    13141 ccctactggt tataatgcaa tgcagtctat cagactcatc agggtgccat tttgtgcata
    13201 tgcggacttt tatgtttcat atctcgaacc tgtgaggcct ctgcttaatc cttaaacaac
    13261 accagcaact cctgcgcttt catcttccag cgaatttttc atgttgctgc tcatcagcca
    13321 taaaatcatt tgcagatgcg ctctgtcgag gtagtctcat aaggttcgtt tatagatcga
    13381 cggcaatgtg agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt
    13441 atggactcca cgctcatctc cactcgtccc gatgaaggga cgctttcgtt aagtcgcgcc
    13501 cgacgagctg cgttaggcag tttcgctggt gccgtcgtcg actggtatga ttttttactc
    13561 tatggcatca ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggct
    13621 atgggaacgc tcgccgcatt tgccaccttt ggcgtcggat tcctgttccg tccgctcggc
    13681 ggtgtcattt tcggtcactt tggcgaccga ctgggacgta agcgcatgtt aatgctgacc
    13741 gtctggatga tgggcatcgc gacagccttg attggtattc ttccttcatt ctcgaccatt
    13801 gggtggtggg cacctatttt gctggtgaca ctgcgtgcca ttcagggatt tgccgtcggc
    13861 ggcgaatggg gaggcgcggc gttgctttcc gttgaaagtg cgccgaaaaa taaaaaagcc
    13921 ttttacagta gcggtgtaca agttggctac ggtgtaggtt tactgctttc aaccggactg
    13981 gtttcattga tcagtatgat gacaactgac gaacagtttt taagctgggg ctggcgcatt
    14041 cctttcctgt ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggag
    14101 gagtccgcgg aatttgaaca acagcaacat aatcaagcgg ccgcgaaaaa acgcatcccg
    14161 gttatcgaag ctctgttacg acatcccggt gctttcctga agattattgc actacgactg
    14221 tgcgagttgc tgacgatgta cattgttact gcctttgcac ttaattattc aacccagaat
    14281 atggggttac cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc
    14341 ctgacaattc cctgttttgc ctggcttgcc gatcgttttg ggcggcgcag ggtttatatc
    14401 acaggtgcgt taatcggaac gttgagcgca tttcctttct ttatggcgct tgaagcacaa
    14461 tctattttct ggatagtttt cttctccatc atgctggcaa acattgcgca tgatatggtg
    14521 gtgtgtgtac aacaaccgat gtttaccgaa atgtttggtg ccagttatcg ctatagtggc
    14581 gctggagtcg gttatcaggt tgccagtgtg gttggcggtg gatttacacc ttttattgcc
    14641 gctgcactca tcacttactt tgccgggaac tggcatagcg tcgccattta tttgctggct
    14701 ggatgcctga tttccgcaat gaccgctttg ttgatgaaag acaatcaacg cgcttaatag
    14761 cctggcgaag atcatccgat cttcgcctta cacttttgtt tcacattcct gtgacatact
    14821 atcggatgtg cggtaattgt atggaacagg agacacacat gaataataag ggctccggtc
    14881 tgaccccagc tcaggcactg gataaactcg acgcgctgta tgagcaatct gtagtcgctt
    14941 tacgcaacgc cattggcaaa tatattacaa gtggcgaatt acctgatgaa aacgcccgca
    15001 aacaaggtct ttttgtctat ccatcactga ccgtaacctg ggacggtagc acaaccaatc
    15061 cccccaaaac gcgcgcattt ggtcgtttta cccacgcagg cagctacacc accacgatta
    15121 ctcgccctac tctctttcgt tcgtatctca atgaacaact tacgttgctg tatcaggatt
    15181 atggtgcgca tatctcggtg caaccctcgc agcatgaaat cccttatcct tatgtcatcg
    15241 atggctctga attgacactt gatcgctcaa tgagcgctgg gttaactcgc tacttcccga
    15301 caacagaact ggcgcaaatt ggcgatgaaa ctgcagacgg catttatcat ccaacggaat
    15361 tctccccgct atcgcatttt gatgcgcgcc gcgtcgattt ttccctcgcg cggttgcgcc
    15421 attataccgg tacgccagtt gaacattttc agccgttcgt cttgtttacc aactacacac
    15481 gttatgtgga tgaattcgtt cgttggggat gcagccagat cctcgaccct gatagtccct
    15541 acattgccct ttcttgtgct ggcgggaact ggatcaccgc cgaaaccgaa gcgccagaag
    15601 aagccatttc cgaccttgca tggaaaaaac accagatgcc agcatggcat ttaattaccg
    15661 ccgatggtca gggtattact ttggtgaata ttggcgtggg accgtcaaat gctaaaacca
    15721 tctgcgatca tctggcagtg ctacgcccgg atgtctggtt gatgattggt cactgtggcg
    15781 gattacgtga aagtcaggcc attggcgatt atgtacttgc acacgcttat ttacgcgatg
    15841 accacgttct tgatgcagtt ctcccgcccg atattcctat tccgagcatt gcggaagtgc
    15901 aacgtgcgct ttatgacgct accaagttag tcagcggcag gcctggtgaa gaagtcaaac
    15961 agcggttacg tactggcacg gtggtaacca cagatgacag gaactgggag ttacgttact
    16021 cagcttctgc acttcgtttt aacttaagcc gggccgtagc aattgatatg gaaagtgcaa
    16081 ccattgccgc gcaaggatat cgtttccgcg tgccatacgg gacactactg tgtgtttcag
    16141 ataaaccgtt acatggtgag attaaacttc ccggccaggc taaccgtttt tatgaaggcg
    16201 ctatttccga acacctgcaa attggcattc gggcgatcga tttgctgcgc gcagaaggcg
    16261 accgactgca ttcacgtaaa ttacgaacct ttaatgagcc gccgttccga taataagtta
    16321 tgaaccttct tatcagaaat agatgtgaag gattaagtaa gacatctggc aaatagcctg
    16381 caataaacgc tggctgtttg cttccttggg cagatgcgag ttttattatc gtcttaatga
    16441 tttccacata ttaaaagc
//
LOCUS       DAZGTJ010000105        16215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000105 DAZGTJ010000000
VERSION     DAZGTJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.105"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3104)
                     /locus_tag="ACHK8E_004221"
     CDS             complement(<1..3104)
                     /locus_tag="ACHK8E_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0346413.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEY"
     gene            complement(3124..3585)
                     /locus_tag="ACHK8E_004222"
     CDS             complement(3124..3585)
                     /locus_tag="ACHK8E_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP0346414.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(3613..5514)
                     /locus_tag="ACHK8E_004223"
     CDS             complement(3613..5514)
                     /locus_tag="ACHK8E_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0346415.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            complement(6258..7706)
                     /gene="cusS"
                     /locus_tag="ACHK8E_004224"
     CDS             complement(6258..7706)
                     /gene="cusS"
                     /locus_tag="ACHK8E_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP0346416.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
     gene            complement(7696..8379)
                     /gene="cusR"
                     /locus_tag="ACHK8E_004225"
     CDS             complement(7696..8379)
                     /gene="cusR"
                     /locus_tag="ACHK8E_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP0346417.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            8536..9918
                     /gene="cusC"
                     /locus_tag="ACHK8E_004226"
     CDS             8536..9918
                     /gene="cusC"
                     /locus_tag="ACHK8E_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP0346418.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            9942..10274
                     /gene="cusF"
                     /locus_tag="ACHK8E_004227"
     CDS             9942..10274
                     /gene="cusF"
                     /locus_tag="ACHK8E_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP0346419.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            10290..11513
                     /gene="cusB"
                     /locus_tag="ACHK8E_004228"
     CDS             10290..11513
                     /gene="cusB"
                     /locus_tag="ACHK8E_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP0346420.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            11525..14668
                     /gene="cusA"
                     /locus_tag="ACHK8E_004229"
     CDS             11525..14668
                     /gene="cusA"
                     /locus_tag="ACHK8E_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP0346421.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            14770..16146
                     /gene="pheP"
                     /locus_tag="ACHK8E_004230"
     CDS             14770..16146
                     /gene="pheP"
                     /locus_tag="ACHK8E_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP0346422.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
BASE COUNT         3533 a         4290 c         4475 g         3917 t
ORIGIN      
        1 tggtactcaa tggcccgtcg gccctgagtg gtgacattca cccgcccgcg ttcgtcatag
       61 ccggtgcgct gtgatgtgcc gtccgggctg aagatacccg tcagcctgcc ggatttgtca
      121 tacgcatact gcgttttgcc ttccggcgtg atgatttctg ccaggcggcc tgcggcgtcc
      181 cggctgtaga ggatggtttc ctccaggccg ttaaccctgg cggtacacag gccatcggcg
      241 tcatagcggt aacgccaggt cacgccgccc cagtcatgct cttccgtcac ccgcccacag
      301 ttgtcgagaa gataacggta tgactcaccc tgcgggttga tgacttctgt cagttgcagc
      361 gtgtctttat catagcggta ctgccagcgg tggccttcac cgtccgttcg cgccaccggc
      421 aaatcaaacg ggccgtattc cattgtccag tccacaccgt taccgtccgt ccaggccagc
      481 aggttgccgt ggcggtcata gcggaatcct tctgcgccgg ttcctgcccg ctcaaggccg
      541 ctcagtttat ggtgctgctg ccactgcaac cgcactgact gaccgccggg gccgcgcatc
      601 ttcaccggca ggtcgtgacc actgtattcg gtgtggtgtt cacggcccat cacgtcggtg
      661 agtgtttccg gccggttgtg gtggtcgtac tctatgccca gacgccgttc atcctggcgg
      721 aatatccccg acagcagccc ctgagcggtg tacgccagcc gggtctcttc gccctgtggc
      781 gataccagac tcaccggcag gccgcgttca tcgcactgct gtgtccacgt ctgcccggca
      841 gggtcggtga tgttgctgag caggccgcgc tcatcatagc ggtacaacca caccgcgccg
      901 tctgccgccg tataacggct taactgaccg tgtacggcgt tgtattcaaa tttctccacc
      961 cgtccggcag gggagtggcg ggccagcagg tgatggaatt catcccattc atacgtggtg
     1021 gtgctgccgt ccggcgcttc ttcccgcaga atattgccgt caggatcata gtaataacgg
     1081 tgaatgccct tgccgtccgt cacggtggtg cagccgtcac tatagtccag cgtggcgttg
     1141 taatagccgt ccgccccggt gacattcacg caccggccct gttcatcgta ggtgaaacgg
     1201 ctccacgtct ggtcgttatc ggaccagcgg atgatccggt ccgcagcgtc gtactcataa
     1261 aacaggtggt agtccagccg cgcatccgct tcggtcagcc gcccccgcgc atcctgttcg
     1321 taggtcgcca gcgtctgtat gccgttatcc gtgcggtgaa tggctttcag gtagccgctg
     1381 gcgtggtacg tcagtttaag ccggatcccg tcggtgtgac tgactcccgt gatgcggtac
     1441 tggctgtcgc gctcaaacgt gatgcggttg ctggcggtgt cgctcatccc gcccagcatg
     1501 tacatccggt caccggggct gtgtgccagg cggtcatcat tggtatcgtc agtcaccggg
     1561 tggcgcaggc gcattcccgg aaaggcgtca taaaagaagc ggctgctcag ggcatcacgg
     1621 tcgaacagct ccaggtaatc gtcgcggcgg cgcaggatat actgggcgtg atacgggtta
     1681 accgccgtat cactgtctcc gtcgaaggca aagtacagcg tggctccctg gctgatgata
     1741 tccacccggt ttccctgttc acgcacccag gcgtattcgc tccaggagtc gctccagccc
     1801 acgcccgtca ggccatgcag tggcagtaca gaactgtgga aacgggtgaa ggagaggggg
     1861 agtgtctggc ccagcgtaaa gtcggtacgt tcgtcgcagt atgcgccggt taccggatcg
     1921 acaggatctg ttacccatct gatgacttta ttggcaatac tttttaacga cttacctgtt
     1981 ttccccagca acgcgccgag ctttgccccc agcagcttcc cgcttttccc cagcaggttt
     2041 cccagtttgc cgagtttcgc cagattcccc agttttccca gcagtcccgc gggcggcaga
     2101 agatagctgg ctatcgttat tccccccagg atcattctct gccacggcgg gaactccggc
     2161 tggatatcca gataggtcac ctgttcaccg ccaataaaga cgtccggcga accttccacg
     2221 attgccgcgc tgcactccag tttgtcgccc ttgcgcgcgg caggctgatt gttgataaag
     2281 acgttgctgc tgccctgggc tatcctcggt ggtttgttct ccttgtcgca gagcgccgtg
     2341 tcctcaccgg cttccgggaa atccacgtct tttttgattt cgccacgccc gcccaccatg
     2401 gtggcaaact ccattaatac cggaccggcg ggaaacagaa gcgatgcgcc ggcgctgacg
     2461 cgatccataa taccggcatt gctgtccgtc agggtgataa cagcgttccc ccaggccttc
     2521 gccagtggca ctgctttttc cgctgccgat tcaagcaggt cagtggcaat atccccccat
     2581 gaaggttccc cctgtggtgg agagggtatt gcttcagtca ggggaggagg gggaagggtg
     2641 acggcggccc tggctgctgg cttaccgtta atttttacgt tttcagaacc ggtattgatg
     2701 gcgccgtccg tgtcacaaaa actgtttatc catgactcaa gctggttctg tttctcctcc
     2761 acataatcac tcgccttata gctggcatac atcagcgcag gcaccagaac cagcgcataa
     2821 gggccggtgg cgcaggcggc cataatcagg caaccttctg cgatagttac tatcgccccc
     2881 agcacggccc ctgccagtgc ccccagaaaa cttttatgct gaatttcatc gcccagcctg
     2941 gcggcaggtg ggccggactt ctttgctgcc agcatctgtg caatggcttt gtcgttggct
     3001 gcccgtttca tctggctggc gaccatcgcc tgctgtgaca gcattcgcat tgccagcgta
     3061 ccgcctgcgg tcgctccctg tgggccgcct ggtccttcac tcatgtgtta tccctcgttt
     3121 atgtcagcgc ggctggtcct tttggatgtc attctccggt ggcaacgggg tgaagctctt
     3181 tatcacctcc agcagcatcg tcttttgact gttcttcagg gataaggagg tgctgaaagt
     3241 gaaggtcagc agcgtatctc cacgccgctg gaatacggct atctggtcaa tccgtccctg
     3301 gttcccctca tcttccggac cgttccagga ataatccagc agccaggccg gggcctctgc
     3361 cagagtgatt tcccggcagg catgttcggt gtacccatca agtttgccct ggtacagcgc
     3421 cagcgtttcc tggtacagct ctggtgtcag ggttccctgt ggaacggctt cgcgggtaat
     3481 gaccaggttg atgccactgt tatctgtgga caccaggacc tgcattgact gatcctgcca
     3541 ttgcgatggc agctgaatat gaccttcatt aaaagtgaac tgcataaaat actccctgat
     3601 aatctgaatg aatcagttga tgaaaacaga accgcccacg atggtgatgc catccttcga
     3661 cagcctgatt accgtcgatt ccccgacctg tagctgaatg ccatccggcg tcagcgcaat
     3721 tttgctttcc tccacggcca gcataatttg cttaattgcc gtataagcct gtataccgtc
     3781 gccttcgcca ccattgttta cagcgtcaac atgcactgaa ccggcaatcg tgcctctgtc
     3841 ctgaaaaaca tcctcagata aagacccttt tagtacgcct gtgattttgt ggcctttttc
     3901 caccgtaaga aggcggttac cttcctggat catgaccgtc tggtcatttt ttacggtttc
     3961 gcggtgatcg tgttttacgg tggtggtacg gtcgttgagt acctcggtat ccatgttctt
     4021 ctgggcgtgg atgtagacct gttctttccc tgtcgcatcg tcaaacttca gctcgttaaa
     4081 cccgctgccc atatatgttt tggagcggat ggtcatctgc gtcttcgttc ccggcaggct
     4141 gcccggcgag cggttatctt cgtggtaggt gcgccccata atgatcggct ggtccggatc
     4201 gccgttgagg aagtccacaa tcacctcctg acccacgcgc ggtatcgcca ggttgccaaa
     4261 tcccggcccc gcccacgcct gcgccacccg tatccagcac gaactgtcct gattggccgg
     4321 gttatagcgg tcccagttga atttcacccg cacccggcca tgctcgtcgc agaagatttc
     4381 ctctcccgca ggccccgtca cgacggcgct ctgcggaccg tccactgacg gctttggctt
     4441 cggctgaggt cgccacgtcc ggtctgccgg tattaccgca aagtggttat tcagcgtggt
     4501 acctgacccc ctgcgtcccg gcaccgcctg cggctgttcg ccgtgcagtt cgctcgccac
     4561 cacctgccat tcccggttca ggttcgcctg cggatgctcc gtcagcacaa ttcgccgtcc
     4621 aggccatatc tccggcgagc ggctcattcc ccgcgcggtt tcggcgttgt ttcgccagcc
     4681 gtccatctgc cagcgggcaa agttctgccc gtgggcgctc ttgaaacgcc cgggccagtc
     4741 atacacttca tactgtgtgc gctggtaatc ctgatgctgg ccttcctggt caaaacgtcc
     4801 ggcccagccg gggcgtttaa aggtgtagtc tttggtcacc acagaggaag gacggatttg
     4861 tgcgctgtag cggaactggc tgatgcagag ggtgctcacc tcggtacggg tgttcgggtt
     4921 ccaggggatt tcaaaggatt ccggcagata gcggaccgtg tcgcacagca ccagactctg
     4981 gtcggtactt ttctgcgcgt gctcctcata gaagaagatg ccttcctccg ctgctatccg
     5041 gcagagaaag tcgtaatcgg tttcaccgta ctggacacaa aactcacggg aaggatgcgg
     5101 ctcactgaac agcgggctcc actccgtgac gtcattttcc tgcagtatgg tgccgaggat
     5161 gcttctgatg tcctcgttct ggaatatacg gaagttctga cgcagtcctg cccgccacag
     5221 cggcgggcgc accttcatgc tgtacagcat ctggtttttg tcgttctccc cgagttcaaa
     5281 ccaggtcacc acgcctttca cccggcgctg tacgtcatcg ccctgccata ccgtcaggta
     5341 ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctgac tgaccagaga
     5401 aaggtcaagg gaaaaaagtg aagagagtga ctgggtgaga tgaaaggaga ccaccgcaaa
     5461 agcgtccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg acatagcaac
     5521 ctccgtgaaa atgaataata atccgcatat attgcatgta caacatgtcg ggtaatgaga
     5581 taagcgggcc ggcttcaggc gttaccgggg aatatcagaa aagtgtcatg gttatgtgat
     5641 gattttgtcc gggattttac ttcagagaaa cggggggctg ttgactcaac aaaataatca
     5701 caaactcaac tgacgggttc acgatgttat taaggacagg ctcgtatatt acatcgagta
     5761 acaaaaaaac acctgatatg caaagtcaaa ccaatatttg taatgtcttt ttattcacaa
     5821 taataaataa ctcaataaac attattgaaa tgaatgatgg tttgtataag gaatatacgc
     5881 taccgcccca ccctgataac atgatctgca cattgctatc ttaacgtcgt tagacgctgg
     5941 cttaatagtg aatattttaa attaggaatt tatcaagttc acttgataaa tagaccgcgt
     6001 gaataatata gagattaatg atgcctgact gcgctttata ttatagccga ttcttttata
     6061 gtgatgaaaa tttagtaata gcattgtgct ctacaattgg tagaaacacg aatttaatta
     6121 aattgcatat ctaaatatct cgtaaatatc cgaaaatgaa taaccttcga tttcagacga
     6181 ccaataaggt cttccctgtt ttattgataa ataataacag aaggcgatac atcactcttt
     6241 gagtttgaac taactaatta acctttctcc ggtagtttga tcataaacct tgtcccccga
     6301 gcatctgacg ttactgcaac cgtgccttta tgcgcgacaa caatcgattt cactatcgcc
     6361 aggccaatac cgctaccttc gccttttcgc tgacgggagg ggtcaacgcg atagaaacgg
     6421 tcaaacaatc gcggtaagtg ctcgggcgca atgggcgtac cggggttttc gacggtaact
     6481 tgcacctggt gatcgaccgt ttggcagcgc acaacaattg cctctccggg tggcgtataa
     6541 cgcagggcgt tcgaaagcag gttgcttaac gcccgacgca gcatcagcgg atcgcccgca
     6601 acctgacact cgtcaccaac aaaccgcaac tccacgccgc gatcttccgc taatgcctcg
     6661 aaaaaatcga acactttgcc gacttcatcc gccaggttga gcattttctt ttcggggatt
     6721 aactggttgt tatcggcctg ggcgagaaac agcatatcgc tgaccatttt cgccattcgc
     6781 gtcagctctt cgagattaga gtagagcaca tcttccagct ccttctggct gcgtgactgg
     6841 ctgagggcga tttccgtttg cgttatgaga ttcgtaattg gtgtacgaat ttcgtgggcg
     6901 atgtccgctg agaaattgga ctggcgggta aagacatcct caatacgctc gatcatatgg
     6961 ttgaacgaca gtaccagctg ttccagctca atgggcacgg tctgcgggtc aaggcgaacg
     7021 tcgagatctt tcgaggtaat gttctggatt tgacggctga cgctgcggat cggcgcgtga
     7081 cctttatgta ccgccaacag cacgataaag acgatcagga tgctgatgat cgatgcggtc
     7141 ataataagtt tattcatcaa atcatttatg taatgaagat gaaaatcgat cgaaagcgca
     7201 atgtagagcg tataaatcgg tttgccgtcc accaacgggc caaccggcaa gctaatcatc
     7261 cgccagttgc tgtgttccat atgcccgtga ccgtggcccg gcatcatcat cgtcgggccg
     7321 gaaaggagat acacctcacc gccccgcgtg tctttatcgg gtatggcgtc acgcgtaaac
     7381 tcgcggatat ccggcgcacc gggggagtga tacaccgttt taccgtggct atctgccagg
     7441 gaaatcaaca cattggaata accactgacg atatcttcca gcgtcattaa gcgttgggct
     7501 tgcgtttcgt cagggtgatt aagtacccgt tcaagagtgg cgctaatctc ttttaaatca
     7561 ttaatatcct gctcggcaaa atgcactttt actgagtgga tcatgatcca tgcaaaggcg
     7621 aaaaacgccg cgatggtggc caggctaata aaaaaggtca ggcgggttgc cagcgaaaac
     7681 gggcgctgaa acggcttact gaccatccgg cacctcaagc atgtaaccca cgccgcgcac
     7741 ggtttgaatc agcttcggct caaagtcgtt gtcgattttg ccacgcagcc gcttcaccgc
     7801 cacatcaatg gcgttagtgt cgctgtcgaa attcatgtcc cacacctgtg aggcaataag
     7861 tgagcggggc agtacttcgc cctgatggcg aaggaaaaac tccagcagag taaattcttt
     7921 actggtcaga gtgatgcgcg tgccactgcg ggtgactttg cggctgacga gatcgaccat
     7981 cagatcggca acctgaaact gactttcgat aatcaccgcc gccccgcgcc gcagtagagt
     8041 gcgcactcgc gccagcagtt cagcaaaagc gaacggcttc accagatagt catccgcccc
     8101 caactccagc cccttgacgc gatgttcaat ggtgccaagc gcggtaagca acagaatcgg
     8161 catcccctta ttggcggaac gcaacatgcg cacgatatcc cagccgttca cgtccggcag
     8221 cataatatcg aggattatca gatcataatc accggtcatc gccagatggt agccattcag
     8281 cccgttgtcg gccaaatcga ccacaaaacc ggcttcggtt aaccctttgg tcaagtattc
     8341 tccggttttc ttttcatctt cgacaatcaa cagtttcata tttcctccgc atgttgcccg
     8401 ggcaattcta gagtagcggg atcagatggc aatcgcttat tggcaaaatg acaattttgt
     8461 cattttcctg tcaccggaaa atcagagcct ggcgagtaaa gttggcggca taaaatcacc
     8521 agaaattatg agcctatgtc tccttgtaaa cttctgccat tttgtgtggc ccttgcgcta
     8581 accggttgtt cactggcacc ggattatcag cgcccggcaa tgcccgtgcc gcagcagttc
     8641 tcactcaacc agaacggcct ggttaacgca gcagataact accagaacgc gggctggcgc
     8701 accttttttg ttgataatca ggtgaagacg ctgattagcg aggcgctggt gaataaccgg
     8761 gatttgcgca tggcgacgct gaaagtgcag gaagcgcggg cgcaatatcg tctgaccgat
     8821 gccgaccgct acccacagct caatggcgag ggcagcggca gctggagcaa caatcttaaa
     8881 ggcgattcag ccacgacgcg ggagttctcg actggcctta acgccagctt tgatctcgat
     8941 tttttcgggc gcttaaagaa catgagcgaa gccgagcgcc aaaattattt agccactgag
     9001 gaagctcagc gcgcggtgca tattttgctg gtttctaatg tcgcgcaaag ctatttcaat
     9061 cagcagctgg cgtatgcgca attgcaaata gccgaagaaa cgctgcgtaa ttatcagcag
     9121 tcatatgcgt ttgtcgaaaa acaactgttg acaggtagca gtaatgttct ggcgctggaa
     9181 caggctcgcg gggtgattga aagtacccgc agcgacatcg ctaaacgtca gggggaactg
     9241 gcgcaggcga ataatgcatt gcaactgtta ttgggaagct acggcaagct gccgcaagcg
     9301 cagacagtaa acagcgacag cctgcaaagc gttaaattac cggcgggctt gtcgtcgcaa
     9361 atcttattgc agcgcccgga tattatggaa gctgaacatg cgttaatggc ggctaatgcc
     9421 aatattggtg ctgcacgcgc ggcatttttc ccgtctatca gcctgaccag cggaatatca
     9481 accgccagca gcgatctgtc gtcattattt aacgccagca gcgggatgtg gaattttata
     9541 cccaaaattg agatccccat ttttaatgcc ggacgtaacc aggccaatct ggatatcgcc
     9601 gaaattcgcc agcagcagtc ggtggtgaat tatgaacaga aaatccagaa cgcctttaaa
     9661 gaagtggcag atgcgctggc attacgtcaa agcctgaacg atcaaattag cgcccagcag
     9721 cgttatctgg cgtcgctgca aattactttg caacgggcgc gggcattata tcagcacggc
     9781 gcggtaagtt atctggaagt gctggatgct gagcgttctt tatttgcaac ccgacaaact
     9841 ttacttgacc tgaattatgc ccgtcaggtt aatgaaattt ctttatatac cgccttaggc
     9901 ggcggttggc agcaataatt tttaactcca ggagagaata aatgaaaaaa gcactgcaag
     9961 tcgcaatgtt tagtctgttt accgttattg gctttaatgc ccaggctaac gaacatcatc
    10021 atgaaaccat gagcgaggca caaccacagg tcattagcgc caccggtgtg gtaaaaacca
    10081 ttgatctgga aagcaaaaaa atcatcatcc accacgatcc aattaccgcg gtgaactggc
    10141 cggagatgac catgcgcttt accatcaccc cgcagacgaa aatgagtgaa attaaaaccg
    10201 gcgacaaagt ggcgtttaat tttgtccagc agggcaacct ttctttatta caggatatta
    10261 aagtcagcca gtaacccagg tttaatgaga tgaaaaaaat cgcgcttatt atcggcagca
    10321 tgatcgcggg cggtattatt tctgcggcag gttttacctg gtttgcaaag gcggaaccgc
    10381 ccgcagaaaa aacgtcgacc gcagaacgta aaatcttatt ctggtacgac ccgatgtatc
    10441 ccaatacgcg gttcgataaa ccaggtaaat cgccgtttat ggatatggat ctggtgccga
    10501 aatatgccga tgaagagagt tctgcgtctg gtgtacgcat tgacccgacc cagacgcaga
    10561 atctgggggt gaaaacggcg accgtaacgc gcgggccgct gacttttgcc cagagtttcc
    10621 cggcgaatgt cagttacaac gagtatcagt atgccattgt gcaggctcgc gctgccgggt
    10681 ttatcgataa ggtgtatccg cttaccgtgg gcgataaagt acaaaagggc acaccgcttc
    10741 tcgacctgac cattcccgac tgggtggaag cgcagagtga gtatttactg ctgcgcgaaa
    10801 ccggcggtac ggcgacccag actgaaggca ttcttgagcg actgcgactg gcgggaatgc
    10861 cggaggcgga tattcgccgc ctgattgcca cgcaaaaaat ccagactcgc tttacgctca
    10921 aagcacccat tgatggcgtg atcaccgcgt ttgatctgcg cgcgggaatg aatatcgcca
    10981 aagataacgt ggtcgcgaaa attcagggta tggacccggt gtggatcact gccgcgatcc
    11041 cggagtccat cgcatggctg gtgaaagatg cctcgcagtt tacgctcacc gttccggcgc
    11101 gacctgataa aacactcacc attcgcaaat ggaccctgct acccggcgtg gatgccgcga
    11161 cccgcacgct gcagctgcgt ctggaagtcg acaacgccga cgagtcgcta aaaccgggca
    11221 tgaacgcctg gttgcaactc aataccgcca gcgaaccgat gctgctcatt ccgtcacagg
    11281 cgctgattga taccggcagt gaacagcggg tgattaccgt tgatgccgac ggacgctttg
    11341 taccgaaacg cgttgccgtt ttccaggcat cgcaaggcgt caccgcgtta cgctctggtc
    11401 tggcggaagg tgaaaaggtg gtttccagcg gcctgttcct gattgattcc gaagccaata
    11461 tttctggcgc actggagcgg atgcgctctg aaagtgctac ccatgcgcat tgagggaata
    11521 accaatgatt gaatggatta ttcgtcgctc ggtggcgaac cgttttctgg tgctgatggg
    11581 cgcgttgttt ctgagtatct ggggcacctg gaccatcatt aacacgccag tggatgcact
    11641 gccggatctc tctgatgtgc aggtgattat taaaaccagc tatcccggtc aggcaccgca
    11701 aatcgttgaa aatcaggtga cttatccgct aaccaccacc atgttgtcgg tgcctggcgc
    11761 gaagactgtg cgcggtttct cgcagtttgg cgactcttat gtgtatgtca ttttcgaaga
    11821 tggcaccgat ccgtactggg cgcgctcgcg ggtgctggag tacctcaacc aggtacaggg
    11881 taagctgcct gcgggagtca gcgccgagct ggggccagat gccacgggtg ttggctggat
    11941 ctatgaatat gcactggtgg atcgcagcgg taagcacgat cttgccgatt tacgctcatt
    12001 gcaggactgg tttctcaaat atgagctgaa aaccattcct gacgttgcgg aagtggcgtc
    12061 ggtaggcggt gtggtgaaag agtatcaggt ggttatcgat cctcagcgcc tggcgcaata
    12121 tggcatcagt ctcgccgaag taaaaagcgc gctggatgct tcaaaccagg aagcgggtgg
    12181 ttcgtcgatc gaactggcgg aagcggaata tatggtgcga gccagcggct atctgcaaac
    12241 gctcgacgac tttaatcaca tcgttttaaa agccagtgaa aatggcgtgc ccgtttatct
    12301 gcgtgatgtt gcgaaggtcc agattggccc ggagatgcgc cggggcattg ccgaactgaa
    12361 cggcgaaggc gaagtggcgg gcggggtggt gatcctgcgc tccggcaaaa acgcccgaga
    12421 agtaatcgcc gccgtgaagg acaaactgga aacgctgaaa agtagtctgc cggaaggcgt
    12481 ggagatagtt acaacatacg atcgcagcca gctcattgat cgcgccatcg acaacctcag
    12541 cggcaagttg ctggaagagt ttattgtggt ggcggtagtc tgtgcgctgt ttctctggca
    12601 tgtgcgctcg gcgctggtgg cgattatttc gttgccgctg gggttgtgta ttgcttttat
    12661 tgtcatgcac tttcagggac tgaacgccaa tattatgtcg ttgggtggca ttgcgattgc
    12721 cgtcggggcg atggtcgatg ccgctatcgt catgatcgag aatgcgcata aacggctgga
    12781 agagtggcag caccagcatc ctgacgccac gttggataat aaaacgcgct ggcaggtgat
    12841 taccgatgca tctgttgaag tggggccggc gctgtttatc agtctgctga ttatcacgct
    12901 gtcgtttatc ccgatcttca ccctggaagg gcaggaaggg cgtctgtttg gcccgctggc
    12961 gttcaccaaa acctatgcga tggcgggtgc ggcgctactg gcgatcgtag tgatcccgat
    13021 cctgatgggc tactggatcc gtggcaaaat tccgccggaa agcagtaacc cgctcaatcg
    13081 ctttttgatt cgtgtttatc atccgctgtt gctgaaagta ctgcactggc cgaaaaccac
    13141 gctgttggtg gcagcgcttt cggtgctgac ggttctctgg ccgctcaata aagttggcgg
    13201 ggaattttta ccgcagatca atgaaggcga cttgttgtat atgccatcga cgctgccggg
    13261 gatttccgca gcggaggcgg cgagtatgct gcaaaaaacc gacaagctga ttatgagcgt
    13321 accggaagtg gcgcgggtat ttggccaaac cgggaaagcg gaaaccgcca ccgattctgc
    13381 tccgctggag atggtagaaa cgaccattca gcttaagccg caggatcagt ggcggcctgg
    13441 catgacgatg gacaaaatca ttgaggaact ggataacacc gtgcgtttgc cggggctggc
    13501 gaatctgtgg gtgccgccaa ttcgtaaccg tatcgatatg ctctcgactg gcattaaaag
    13561 tcccatcggt attaaagttt ccggcaccgt gctggcggat atcgacgcga tggcggagca
    13621 aattgaagaa gtggcgcgaa cggtgccagg cgtggcttct gcgcttgctg agcgtctgga
    13681 aggtgggcgt tatatcaacg ttgagattaa ccgtgaaaaa gccgcgcgtt acggtatgac
    13741 ggtggcggat gtgcagttgt ttgtgacttc tgcggtgggc ggggcgatgg ttggcgaaac
    13801 ggtggaaggg attgctcgtt atccaattaa tctgcgttat ccgcaaagct ggcgcgatag
    13861 cccgcaggca ctgcgccagt tgccgatcct cacgccgatg aagcagcaaa tcaccctggc
    13921 agacgtggct gacgttaaag tctctaccgg accgtcgatg ctgaaaaccg agaatgcgcg
    13981 cccgacgagc tggatttata tcgatgcccg cgatcgtgac atggtgtcgg tggttcacga
    14041 tctgcaaaaa gcgatagctg aaaaagtgca gttaaaaccg ggcaccagcg tggcattctc
    14101 cgggcagttc gagctactgg agcgcgccaa ccataagctg aaactcatgg tgccgatgac
    14161 gctgatgatc atcttcgtgc tgttatatct ggcgttccgt cgggtgggcg aagcgttgct
    14221 gattatcagc agcgtaccgt ttgcgctggt gggcggcatc tggttgctgt ggtggatggg
    14281 ctttcatctt tccgtggcga cgggcaccgg ttttattgcc cttgccgggg ttgccgccga
    14341 atttggcgtg gtgatgctga tgtatttacg tcacgccata gaggccgagc cgtcgttgaa
    14401 taatccgcaa acattcagcg agcagaagct ggatgaggcg ttgtatcacg gcgcggtcct
    14461 gcgcgtgcgc ccgaaagcga tgacggtggc agtgattatc gccggtctgc tgccgattct
    14521 gtggggaacg ggggctggtt cagaggtgat gagccggatt gccgcaccga tgattggcgg
    14581 catgatcacc gcacctttgc tgtcgctgtt tattatcccg gcggcgtata agctgatgtg
    14641 gctgcaccga catcgggtac ggaaataaaa gcaggatacc ccgtttaacc gtgtggattg
    14701 tgtcttgcga cgatgggcac taaatgttaa aaggtgcccc tcaacaaaaa agacacacag
    14761 gggaaaggcg tgaaaaacgc gtcaaccgta tcggaagata ctgcgtcgaa tcaagagccg
    14821 acgcttcatc gcggattaca taaccgtcat attcaactga ttgcgctggg tggcgcaatt
    14881 ggtactggtc tgtttcttgg cattggtccg gcgattcaga tggcgggtcc ggctgtattg
    14941 ctgggctacg gcgtcgccgg gatcatcgct ttcctgatta tgcgccagct tggcgaaatg
    15001 gtggttgagg agccggtatc cggttcattt gcccactttg cctataaata ctggggaccg
    15061 tttgcgggct tcctctctgg ctggaactac tgggtaatgt tcgtgctggt gggaatggca
    15121 gagctgaccg ctgcgggcat ctatatgcag tactggttcc cggatgttcc aacgtggatt
    15181 tgggctgccg ccttctttat tatcatcaac gccgttaacc tggtgaacgt gcgcttatat
    15241 ggcgaaaccg aattctggtt tgcgctgatt aaagtgctgg cgatcatcgg tatgatcggc
    15301 tttggcctgt ggctgctgtt ttctggtcac ggcggcgaga aagccagtat cgataacctc
    15361 tggcgttacg gtggtttttt tgccaccggc tggaatgggc tgattttgtc gctggcggta
    15421 attatgttct ctttcggcgg tctggagctg attgggatta ctgccgctga agcgcgcgat
    15481 ccggaaaaaa gcattccgaa agcagtgaat caggtggtgt atcgcattct gctgttttat
    15541 atcggttcac tggtggtttt actggcactc tatccgtggg tggaagtgaa atccaacagt
    15601 agcccgtttg tgatgatttt ccataatctc gacagcaacg tggtagcttc tgcgctgaac
    15661 ttcgtcattc tggtggcatc gctgtcggtg tataacagcg gggtttactc taacagccgc
    15721 atgctgtttg gcctttctgt gcagggtaat gcaccgaagt ttttgactcg cgtcagccgt
    15781 cgcggcgtgc cgattaactc gctgatgctt tccggagcca tcacttcgct ggtggtgctg
    15841 attaactatc tgctgccgca aaaagcgttt ggtctgctga tggcgctggt ggtagcaacg
    15901 ctgctgttga actggattat gatctgcctg gcgcatctgc gttttcgcgc ggcgatgcga
    15961 cgtcagggac gtgaaacaca gtttaaggcg ctgctctatc cgttcggcaa ctatctttgc
    16021 atcgccttcc tcggcatgat tttgctgctg atgtgcacga tggatgatat gcgcttgtca
    16081 gcgattctgc tgccagtgtg gattgtattc ctgtttgtag cgtttaaaac gctgcgtcgg
    16141 aaataaggca ttcgcgccgt atccgacaaa atgatgtcaa ccatccgaaa ccgctctcat
    16201 ccattcgatg agagc
//
LOCUS       DAZGTJ010000106        15518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000106 DAZGTJ010000000
VERSION     DAZGTJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.106"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            194..1051
                     /gene="focA"
                     /locus_tag="ACHK8E_004231"
     CDS             194..1051
                     /gene="focA"
                     /locus_tag="ACHK8E_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP0346423.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1106..3388
                     /gene="pflB"
                     /locus_tag="ACHK8E_004232"
     CDS             1106..3388
                     /gene="pflB"
                     /locus_tag="ACHK8E_004232"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0346424.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3580..4320
                     /gene="pflA"
                     /locus_tag="ACHK8E_004233"
     CDS             3580..4320
                     /gene="pflA"
                     /locus_tag="ACHK8E_004233"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP0346425.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4419..4529
                     /locus_tag="ACHK8E_004234"
                     /pseudo
     CDS             4419..4529
                     /locus_tag="ACHK8E_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4530..5960)
                     /gene="ycaM"
                     /locus_tag="ACHK8E_004235"
     CDS             complement(4530..5960)
                     /gene="ycaM"
                     /locus_tag="ACHK8E_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0346426.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(6170..7318)
                     /gene="ycaD"
                     /locus_tag="ACHK8E_004236"
     CDS             complement(6170..7318)
                     /gene="ycaD"
                     /locus_tag="ACHK8E_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346427.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7633..8259
                     /gene="ycaC"
                     /locus_tag="ACHK8E_004237"
     CDS             7633..8259
                     /gene="ycaC"
                     /locus_tag="ACHK8E_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP0346428.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(8295..9158)
                     /gene="dmsC"
                     /locus_tag="ACHK8E_004238"
     CDS             complement(8295..9158)
                     /gene="dmsC"
                     /locus_tag="ACHK8E_004238"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP0346429.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9160..9777)
                     /gene="dmsB"
                     /locus_tag="ACHK8E_004239"
     CDS             complement(9160..9777)
                     /gene="dmsB"
                     /locus_tag="ACHK8E_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP0346430.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(9788..12232)
                     /gene="dmsA"
                     /locus_tag="ACHK8E_004240"
     CDS             complement(9788..12232)
                     /gene="dmsA"
                     /locus_tag="ACHK8E_004240"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP0346431.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(12532..13524)
                     /locus_tag="ACHK8E_004241"
     CDS             complement(12532..13524)
                     /locus_tag="ACHK8E_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0346432.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIEAWWLLDGRNQDYGDTYRVRLEKINREMGDPRASQMT
                     RKFMLGYRSDKLQAGLMPSSINRDLCALSAMFSSLIEGEVFHNENPVRGIRKLKVRNT
                     EMAFLSDEEIERLLERLDGDARRVAILCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDAVLALIKTRRTGLLYQADYLRFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLAESVHKLSID"
     gene            complement(13594..13935)
                     /locus_tag="ACHK8E_004242"
     CDS             complement(13594..13935)
                     /locus_tag="ACHK8E_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0346433.1"
                     /translation="MVILSIDYAEKLRQIRKAEGLTQKQFAEITGLSLATVRNYESGQ
                     KSARAKIVESVLQADRFEKYMFWLLKDKTLPAAGQIAPALSLDGSSQSEGDLASIDVT
                     QKSSRSGRNAG"
     gene            14039..14566
                     /locus_tag="ACHK8E_004243"
     CDS             14039..14566
                     /locus_tag="ACHK8E_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP0346434.1"
                     /translation="MRDFIADKELSDTDHTTGAAYINADTSQQEATDNNDEQKPQRTY
                     KKGATLRLDGPIAGLCSLEKGAAYIGLTKTALRAALQRNQMPGHKTRSNPEDENSDGV
                     WWFNVREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFTPADKEDCQTVNGKRVYKG
                     RKSKLEQSQDVNERG"
     gene            14570..14965
                     /locus_tag="ACHK8E_004244"
     CDS             14570..14965
                     /locus_tag="ACHK8E_004244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346435.1"
                     /translation="MSGKNKSEKTATVLKQLSLLKEEHKDATILMHLESSPKITITTT
                     YSELHKELEGLINFCSKLKANENIDDLILKPEAEFGHFCENRENGCIGIMLYATRGIM
                     INLPVEQAVDIFGEIALNKTTIYKRKMPE"
     gene            15269..>15518
                     /locus_tag="ACHK8E_004245"
     CDS             15269..>15518
                     /locus_tag="ACHK8E_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0346436.1"
                     /translation="MTQRRSLRTGTERHAARFSTSASRSNSHYSLQETHATPDGHPVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQF"
BASE COUNT         4205 a         4011 c         3605 g         3697 t
ORIGIN      
        1 cggaaaaaat tcaactcatt tgttaatttt taaaatttat ttttatttgg ataatcaaat
       61 atttactccg tatttgcata aaaaccatgc gagttacggg cctataagcc aggcgagata
      121 tgatctatat caatttctca tctataatgc tttgttagta tctcgtcgcc gacttaataa
      181 agagagagtt agtgtgaaag ctgacaaccc ttttgatctt ttacttcctg cagcaatggc
      241 caaagtggcc gaagaggcag gtgtctataa agcaacgaaa catccgctta agactttcta
      301 tctggcgatt accgccggtg ttttcatctc aatcgcattc gtcttctata tcacagcaac
      361 cactggcaca ggaacaatgc ccttcggcat ggcaaaactg gttggcggca tttgcttctc
      421 tctggggctg attctttgtg ttgtctgcgg agccgatctc tttacttcca cagtgttgat
      481 tgttgttgct aaggcgagtg ggcgcatcac ctggggtcag ttagcgaaaa actggctaaa
      541 tgtctatttt ggcaacctgg tcggcgcact gctgtttgta cttttaatgt ggctttccgg
      601 cgagtatatg accgcgaatg gtcaatgggg actaaacgtc ctacaaaccg ccgaccacaa
      661 agtgcaccat acttttattg aggccgtctg tcttggtatc ctggcaaacc tgatggtatg
      721 tctggcagta tggatgagtt attctggccg cagcctgatg gataaagcgt tcattatggt
      781 gctgccggtc gcgatgtttg ttgccagcgg ttttgagcac agtatcgcaa acatgtttat
      841 gatcccgatg ggtattgtaa tccgcgactt cgcatctccg gaattctgga ccgctgtcgg
      901 ttctgcaccg gaaaattttt ctcacctgac cgtgatgaac ttcatcactg ataacctgat
      961 tccggttacg atcggtaata ttatcggcgg tggtttgttg gttgggttga catactgggt
     1021 catttatctg cgtgaaaacg atcaccatta atggttgtcg aagtacgcag taaataaaaa
     1081 atccacttaa gaaggtaggt gttacatgtc cgagcttaat gaaaagttag ccacagcctg
     1141 ggaaggtttt accaaaggtg actggcagaa tgaagtaaac gtccgtgact tcattcagaa
     1201 aaactacact ccgtacgagg gtgacgagtc cttcctggct ggcgctactg aagcgaccac
     1261 caccctgtgg gacaaagtaa tggaaggcgt taaactggaa aaccgcactc acgcgccagt
     1321 tgactttgac accgctgttg cttccaccat cacctctcac gacgctggct acatcaacaa
     1381 agcgttggaa aaagttgttg gtctgcagac tgaagctccg ctgaaacgtg ctcttatccc
     1441 gttcggtggt atcaaaatga tcgaaggttc ctgcaaagcg tacaaccgcg aactggaccc
     1501 gatgatcaaa aaaatcttca ctgaataccg taaaactcac aaccagggcg tgttcgacgt
     1561 ttacactccg gacatcctgc gttgccgtaa atccggtgtt ctgaccggtc tgccagatgc
     1621 ttatggccgt ggtcgtatca tcggtgacta ccgtcgcgtt gcgctgtacg gtatcgacta
     1681 tctgatgaaa gacaaatacg ctcagttcac ctctctgcag gctgatctgg aaaacggcgt
     1741 aaacctggaa cagactatcc gtctgcgcga agaaatcgct gaacagcacc gcgctctggg
     1801 tcagatgaaa gaaatggctg cgaaatacgg ctacgacatc tctggtccgg ctaccaacgc
     1861 tcaggaagct atccagtgga cttacttcgg ctacctggct gctgttaagt ctcagaacgg
     1921 tgctgcaatg tccttcggtc gtacctccac cttcctggat gtgtacatcg aacgtgacct
     1981 gaaagctggc aagatcaccg aacaagaagc gcaggaaatg gttgaccacc tggtcatgaa
     2041 actgcgtatg gttcgcttcc tgcgtactcc ggaatacgat gaactgttct ctggcgaccc
     2101 gatctgggca accgaatcta tcggtggtat gggcctcgac ggtcgtaccc tggttaccaa
     2161 aaacagcttc cgtttcctga acaccctgta caccatgggt ccgtctccgg aaccgaacat
     2221 gaccattctg tggtccgaaa aactgccgct gaacttcaag aaattcgctg ctaaagtgtc
     2281 catcgacacc tcttctctgc agtatgagaa cgatgacctg atgcgtccgg acttcaacaa
     2341 cgatgactac gctatcgctt gctgcgtaag cccgatgatc gttggtaaac aaatgcagtt
     2401 cttcggtgcg cgtgcaaacc tggcgaaaac catgctgtac gcaatcaacg gcggcgttga
     2461 cgaaaaactg aaaatgcagg ttggtccgaa gtctgagccg atcaaaggcg atctcctgaa
     2521 ctacgatgaa gtgatggagc gcatggatca cttcatggac tggctggcta aacagtacat
     2581 cactgcactg aacatcatcc actacatgca cgacaagtac agctacgaag cctctctgat
     2641 ggcgctgcac gaccgtgacg ttatccgcac catggcgtgt ggtatcgctg gtctgtccgt
     2701 tgctgctgac tccctgtctg caatcaaata tgcgaaagtt aaaccgattc gtgacgaaga
     2761 cggtctggct atcgacttcg aaatcgaagg cgaatacccg cagtttggta acaacgatcc
     2821 gcgtgtagat gacctggctg ttgacctggt agaacgtttc atgaagaaaa ttcagaaact
     2881 gcacacctac cgtgacgcta tcccgactca gtctgttctg accatcactt ctaacgttgt
     2941 gtatggtaag aaaactggta acaccccaga cggtcgtcgt gctggcgcgc cgttcggacc
     3001 gggtgctaac ccgatgcacg gtcgtgacca gaaaggtgca gtagcctctc tgacttccgt
     3061 tgctaaactg ccgtttgctt acgctaaaga tggtatctcc tacaccttct ctatcgttcc
     3121 gaacgcactg ggtaaagacg acgaagttcg taagaccaac ctggctggtc tgatggatgg
     3181 ttacttccac cacgaagcgt ccatcgaagg tggtcagcac ctgaacgtta acgtgatgaa
     3241 ccgtgaaatg ctgctcgacg cgatggaaaa cccggaaaaa tatccgcagc tgaccatccg
     3301 tgtatctggc tacgcagtac gtttcaactc gctgactaaa gaacagcagc aggacgttat
     3361 tactcgtacc ttcactcaat ctatgtaatt agatttgact gaaatcgtac agtaaaaagc
     3421 gtacaataaa ggctccacga aagtggggcc ttttttagca cgagagcctt ttttgtcagc
     3481 tatctatact ttaaggtgac tgccaaaaca gactcgacgt agccttcgag ctgcgcacca
     3541 acacggcctc agatgggcca catctggaga aacaccgcaa tgtcagttat tggtcgcatt
     3601 cactcctttg aatcctgtgg aaccgtagac ggtccgggta ttcgctttat cacctttttc
     3661 cagggctgcc tgatgcgctg cctgtattgt cataaccgcg acacctggga cacgcatggc
     3721 ggtaaagaag ttaccgttga agatttaatg agagaggtgg tgacctatcg ccactttatg
     3781 aacgcttccg gcggcggcgt taccgcatcc ggcggtgaag caatcctgca agctgagttt
     3841 gttcgtgact ggttccgcgc ctgcaaaaaa gaaggcattc atacctgtct ggacaccaac
     3901 ggttttgttc gtcgttacga tccggtgatt gatgaactgc tggaagtaac cgacctggta
     3961 atgctcgatc tcaaacagat gaacgacgag atccaccaaa atctggttgg agtttccaac
     4021 caccgcacgc tggagttcgc caaatatctg gcgaacaaaa atgtgaaggt gtggatccgc
     4081 tacgttgttg tcccaggctg gtctgacgat gacgattcag cgcatcgcct cggtgaattt
     4141 acccgtgata tgggcaacgt tgagaaaatc gagcttctcc cctaccacga actgggcaaa
     4201 cacaaatggg tggcgatggg tgaagaatac aaacttgatg gtgttaaacc accgaagaaa
     4261 gagaccatgg aacgcgtgaa aggcattctt gagcagtacg gtcataaggt aatgttctaa
     4321 tttccccgta aagcggcaac ttttttgagt tgccgctttt ttattccgcg ctgcataaat
     4381 aattcagagc tctatttcta acgcccttac agcacagctt ccaaatgtat tacccaaacc
     4441 gccagtatat gtcctgcggc ttgcgcgcat ttctcgatta tgtaaaaacg ggtcagatct
     4501 gccagagtca gcatcaccga ccacaataat tatttgctat tcgctttacg cgccagcagc
     4561 gggaatatca gccctaatcc taccagcaca aacggcgttg caacattcag cgccaactgg
     4621 aatgtccact ctgcggtgaa ggcttccatt ttcgggaaaa taccagtcag gcaggcaaaa
     4681 gcggtaaagg caaaacacca aattccgacg gtcattgcca gcggcttatt acgaataaag
     4741 acatattccg gcttatattt ctgcgccagg cgaacaactg caataaatgc aacaaatacc
     4801 cataaataac gcagcggcat cactaccgag ttaagattca acagccattt atagagattg
     4861 ttcatatcac caatgccgag agtcggtagc attatcagga tcgccaccag caccagggtc
     4921 agaaaatagc cattaacggg cgtaccagaa gcgttggtac gacataaact ggctggaata
     4981 tatttgctgt cagcatcgcc taatagcact ttaagcgggg catcaatcga gaataccagc
     5041 gcagctactt gtcccagggt attcgcaatg gcgtaaatca ccattaaagt attacccatg
     5101 ttgtaatact cgcccagctt ctgaaaggcg taatactgac cgttagtcat taagtcatcc
     5161 gggatattac gcgagtcaaa catcatcccc atcgccagtg aacccagaat ggcacaaacc
     5221 gcaaccatca ccgccaggca taacatccct tttggaaatt cttttcctgg gttgcgcgtt
     5281 tgattaacgt aaggagaaat cttctctgct ccgccaaccg cgaaaaccag cattgaaata
     5341 gtggtaatgt aggtaaagtc gatatgagga atgaacgttt cccaggtaat gtttgtggtc
     5401 gcaatatgca cttcagtaat tgcaggcgcg gttaccgcca tcgccacata caggagcgac
     5461 ataacaaaca tcgcaatccc tgccacagaa ccgacgattt tcagcgattt cataccgcgt
     5521 gaagcaaccc acataaagaa gataaacagc accagcgtta acccctgtaa cgctacgact
     5581 gaatattctt tgattaacga accgtcgcct ttcatcgccc aaccgagcgc aatcagtatt
     5641 gcctggggtt tttgtgccag atagggaata tgcactaccc agtaggtcca cgcggcgagg
     5701 taagccagtc cgggtcccat cgtgtgttta atccaggtac tgaccccgcc cttcccatct
     5761 ttgaaggtcg agcctaactg accaacaatt agcgcataag gtgtgaaata gagcgcaaag
     5821 ataaacaccc atgaaaaaac aaccaccagc ccctggttgg cgtagttatt aacaacattg
     5881 ccaaaacccc agacagtgat aaaagacatc agcgcaatgt tgtaccatcg cagctgtttt
     5941 tcctgaacat tcccagccat agacaaatcc ttcgccaaaa ttaaaggggc atctgtaaaa
     6001 cgagggatta tgcgcggcaa ataagaagtg cggaaatagc gaaatgcaaa attataacct
     6061 gctgcacatt ttatttttaa acgaataaaa tttggaataa agcaagtcag atagaagaaa
     6121 caaaaaagca gagtgtgaaa cagtaagttc acactctgct tgaattcatt tacacgtgag
     6181 caacgggttt cggcgtatga ccggcgttgc gaagcagcat cagcaaatag ataaacgata
     6241 cgctggcgat catgataaac aataaattat cggagaaatt ctgcattagc atagcggtaa
     6301 atgacgggcc aagcagactt cccacggtat agctcaacag taaggcctgg ttcatcgcca
     6361 ccagttgatg atgttcaact ttctcacaag cccaggccat cgccaccgga tatagcgtaa
     6421 agccagcggc accgaggatg aataacgctg gggccatcgc cgcctggcta agcatcgcga
     6481 tactgccgag aatgacgaca aagacctgaa cacgcaacac cagcagtcga ccaaacttat
     6541 ccgccagacg tccgatcggc cattgtccga ggatacccgc actgaccagt accgccatcc
     6601 agaaaccaat gctggcattg ctcacccctt tgtgattgag gtacagcggc atcaggccat
     6661 atagagatcc cagcacgatt cctgagataa tgcagccatt cacgccaagc cgcgcctgac
     6721 ggagttttag cattgacgta attgacgtcg aatcatggtt ttcagcctgc tgattcagca
     6781 cacgcgtaaa caacagcggt aagatccctg ccaacgtcaa acctgtaacc cacggcaata
     6841 cggacatcag ctcggttgaa actttgctga ccagtaactg gcctaaaaac gttcccacgt
     6901 aataaaccat catatacgca gcaagcaaac gcccacggtt acgtgacgtc ccactgcaca
     6961 tcagcgcgct ctcaacaacc acccaaatca tggcacaacc gacaccggcg acaaaacgcc
     7021 aagccaacca gctccagaat ccaatcatca ggccaaggcc ggcacagcca gcggcaaaaa
     7081 tgaaggaggc cagataatag ctgcggttaa agccaatgcg cttaatgaca taccctgtca
     7141 gcaatgtacc gaccaggttg ccggtaaaat aggatgagct gacaacgcct acctgccatg
     7201 tggacatgtg ttcctgggcg agccaaagcg gcacgagtgt atttaacact gcgatcgcca
     7261 gagtcaacaa aagcaggcca gacagcaaaa gcatgacagg ccgggtatac gtggacatga
     7321 attaaaaccg tgaggaagtt caaatttcat gcgcatcatg ccaccgccaa aaacattgtc
     7381 aatgggtgaa aatacgcgca tatcgcagcg gaggtattat cgatttatat tactcattca
     7441 taacgctaat gctggagaga cgattaatct catctgattg tttttattga tctaacacaa
     7501 atttcatacg cttcgttcag tcgaaaaatt tcatggtcac agcaaactca tatttcccgt
     7561 ctatgctttc agagcaatgc atcgcgtctg accgatttca gacgtaccac tgactctcga
     7621 aaggagaaga ggatgaccaa accgtatgtt cgtcttgata aaaatgatgc tgccgttttg
     7681 cttgttgatc accaggctgg tttactttcc cttgtacggg atatcgaacc cgataagttt
     7741 aaaaataacg tgctggcgct gggtgacctg gctaagtatt tcaacttacc aactattctt
     7801 accaccagtt ttgaaaccgg tcctaacggc ccattagttc cagaactgaa agcacaattc
     7861 cccgatgcac cttacattgc tcgcccggga aatattaacg cctgggataa cgaagatttt
     7921 gtaaaagctg tcaaagcgac aggtaaaaaa cagttaatta ttgccggtgt ggtaaccgaa
     7981 gtttgcgtgg cattcccggc gctttcagcc attgaagaag ggtttgacgt ctttgtcgtg
     8041 actgacgcgt cagggacctt taatgaaatt acccggcatt cggcatggga tcgcatgtcg
     8101 caagctggtg cgcaattgat gacatggttc ggcgtggcat gcgagctgca tcgcgactgg
     8161 cgtaatgata ttgaaggact ggcgacgttg ttctctaacc acattcctga ttatcgtaac
     8221 ttgatgacca gttatgacac gttaacgaag cagaaataaa agcgaaccgg ggccgaaacc
     8281 ccggttgtta ctgattaact tgcgacagcc atccccacgg tcatatgcaa accatagaat
     8341 acgccacgac cgattaattc ccctgccaga agcagaatga atgatacaga aagtagcgga
     8401 accgcaggct gataaccttt tagctgtggt gcaatccaca agcacagggc aacagccaaa
     8461 agcacgatcc gccaggacat cagcgcgcca tagtccggca ccagcgctgc ggcctgctgc
     8521 acagagctat gaatagtcgc cagctctgcg ccctgcatca ctgacatcac gccactcact
     8581 accagtgcca gtacagaaat ggctggcagc agacgcatcg cccagccatc gacgccagcc
     8641 agactcagca acaggtaacc cagtaacggg ccgcccatga acatcgtcag gaagaagccc
     8701 atcggcgtcc agatgctgta ccaggtcgga acggtatcaa tgctgttata cacacgcacc
     8761 atcatccaga caaagatgac gccaagaacc atcgtcacta tcagccacag cgtacgcaat
     8821 gccggtgaca gctttttcag cattgccagc aaccagccga tgccaccaac agcaaaaaag
     8881 atcgaaccgc tggcgatttc gttactgagt gctgaagccc ctacccggtt gagcgagtta
     8941 aaggcgcgca tcggtgaacc aagatgaagc atagaggcga taaagccaat gcccatcagc
     9001 acccataaac caaacatgca ggcaataaca cgctgctggg cttctgctcg caggtcgcct
     9061 ttgagcagcg ccaaagccag aacgataaaa ccacccgcta cacattgccc gaagaccgtg
     9121 aagatcatca gcggccattc atgccatcca cttcccatct cacacctcct tcgggtttgc
     9181 cagatagcca gtggtatccc cggtcgggcg gctgttggca ttgggtttga tcacaatatt
     9241 cggtttggta aagtgagctc gcggcaacgg cgcaacggcc gccagatcgc catgtttttt
     9301 acgcagctcg tcgatagggc cgaaatccag cgcccgcagc gggcaagatt caacacagat
     9361 cggctttttc ccctccgcaa cacggtcata acaaccatcg catttggtca tatggccttt
     9421 cgtttcgtta tattgcggtg cgccgtacgg gcaagccata tggcagtaac ggcagccaat
     9481 gcacacatct tcatcgacca caacaaaacc atcttcacgt ttatgcatcg caccgctcgg
     9541 gcagacttta gtacaagccg gatcttcgca gtggttacat gaaatcgaca gatagtaggc
     9601 aaacacgttc tggtgccaga caccgttatc ttcctgccag tcgccgccag catattcata
     9661 aatgcggcgg aaactgactt ctggcgtcaa atctttgtag tctttacaag ccagctcgca
     9721 ggttttgcaa ccggtgcaac ggctggaatc aataaaaaat ccatactggg ttgtcatcgg
     9781 ttactcctta caccttttca acctgaacca ggtttgtatg tgacggattc cccttagcga
     9841 gaggagacgg acgttgagtg gtcagtacgt tgatacaacc gcccttatcg acacgttttg
     9901 tatccgggtc ataccaggca ccttcaccca gtgcgaccac acccggcatc attcgtggcg
     9961 tcactttcgc ctcaatatga acctcaccac gatcgttaaa gatcctgact ttatcgccgt
    10021 tgttgatacc gcgtttttgc gcatcaagcg ggttgatcca catttcctga cggcaagctg
    10081 ctttcagcac atcaacgttg ccgtaagttg agtgaacgcg agatttatag tggaaacccg
    10141 taagctgcag cggatactgt ttgttcagcg gatcctgata actttcaaag cccggcgtgt
    10201 agatcggcag tggatcgatc acatcacctt ctggcaattc ccaggtagcg gcaatgtcag
    10261 ccagcgcctg cgaataaatc tcaattttcc ccgatggcgt ggtcagtgga tttgcctgcg
    10321 gatcttcacg gaaagcttta taagcaacgt gatgcccttg tgggtcgcgc tttttaaaga
    10381 tcccctgctt acggaactct tcaaacgttg gcagttcagg aatcgcttcc cgcgactggg
    10441 catacagatg ccgcatccac tcttcctggg tacggccctc agtaaactgt tgctcaacgc
    10501 caagacgttt tgccagttcg ctggtcattt catagatggt cttacattca aagcgcggtt
    10561 taatcacctg atcgttgaaa atcacgtaag acatattccc gcaggatgca tccagtgcaa
    10621 agtccatctg ttctgaagcg gtgcagtcag gcagcaggat gtcagcatat ttcgccgatg
    10681 aggtcatgtg gcagtcgata accacaatca gctcgcactt cttatcatcc tgaaggattt
    10741 catgggtacg gttgatttca gaatgctgat taatcaggca gttaccggca tagttccaga
    10801 tcattttgat cggcacatcc agcttatctt tcccgcgcac accatcacgc agcgctgtca
    10861 tttccgggcc acgttcaatg gcatcggtcc acataaacat cgaaatgctg gtctggatcg
    10921 ggttttcgag ggtcggcata cggacaaacg gtaagctgta tgaaccttca cgcgcgccgc
    10981 tgttgcctcc gttaatacca acgttaccgg tcagaatcgc cagcatcgag atagcacggg
    11041 ttgcgatttc accgttagcg tgacgctgcg ggccccatcc ctggctgata aacgccggtt
    11101 tggtactacc gatttcacga gccagtttga tgattttgtc tgccggaaca ccagtgattt
    11161 gcgaggccca ttccggcgtt ttagccacgc catctggccc ttcacccaga atataagctt
    11221 tatagtgacc atttttcggc gcactggctg gcagtgtttt ctcgtcgtag ccaacgcaat
    11281 atttatcgag gaatgcctga tccaccaggt tttcggtgat catgacatac gccagaccgt
    11341 taaccagtgc ggcatctgtt cccggacgaa tagggatcca ctcatcttcg cgcccggcac
    11401 cggtgtcggt atagcgcgga tcgatgatga tcatgcgagc attagatttc tggcgcgcct
    11461 gttcaagata gtaagtcacc ccaccgccac tcattcgcgt ttcgccaggg ttattaccaa
    11521 acagcactac cagtttactg ttttcgatat ccgacgggct gttaccatct gcccagccgc
    11581 cgtaggtata gttcaaacct tccgcaattt gcgcggaaga gtagtcaccg taatggttca
    11641 gataaccgcc gcagcagttc atcaggcgag cgaccagggt atttcccggc ggccaggagc
    11701 gggtcatggt gccgcccagc gttccggtac catagttcag atagatagac tcgttgccgt
    11761 actctttgat caggcgctgc atattggtcg cgatgatgtc gtaggcttct tcccagctaa
    11821 tgcgctcgaa tttgccttca ccgcgcgccc cgactcgttt catcggatat ttcaggcggt
    11881 ccggattgta gacacggcga cgcatggaac gaccacgcag acaggcgcgc acctggtgca
    11941 ggccgtcata gttgtcatcg ccggtattat ccgtttcaac atatttaatt tcaccgtcca
    12001 caacgtgcat acgtagcggg cagcgactac cacagttaac tgtacaggcg ctccagataa
    12061 ccttttcgtc tgattttgcc ggaagggcac tatcaacagc gtgcgcaatc cgactaaaag
    12121 gtaatgttaa cgcgctgctt gccattgcca ggccgccgat cgctgtcgtt tttaccaaac
    12181 cacggcgact cacctcagca gccaataccg catcagggat tttcgttttc ataatggctc
    12241 actcaagctt gctcacgatt gattccagta acaaaccgta ttgtgtaatt atttctatag
    12301 cagttcgcta tataggcttg tatacatcga ataatttgaa aatcaaagag ataggtagat
    12361 tttcactcga aagtagtatt acgtgtaagt gaggagtaat tattgttcgg tatcaaaggg
    12421 agacaaaaaa agcgcccgca ggaggtttaa cgctacttac tccccttgat gatttgctta
    12481 tttttcaatg agtaaatcaa aacaagggga gcaaataagc acaagttaca actaatcgat
    12541 ggacagttta tggacacttt cagccagcgg attaaagctg attgcatcct gtaaaaagtc
    12601 cggcgaaaaa tgcgcatacg tcatcgtttg ttgaatcgtg gcatggccca ggattcgttg
    12661 tagcgtaacg atatttccgc cattcatcat gaaatgtgta gcgaatgtat gacgcatgac
    12721 atgagtggcc tgccctttcg gcaagtccgg cttcacctcc tgaaggatct cccgaaatct
    12781 gaggtaatca gcctggtaca acaatccggt tctgcgggtt ttaattaacg caagtaccgc
    12841 atcagaaacg ggaacggcgc gagattttcc gttcttggtg ttaaagaaca tcacgcggtt
    12901 acctacaata tgttccccac gtaaaccagc tgcttcgccc caccttgccc ccgtggaaag
    12961 acacagaatg gcaacacgcc gcgcatctcc atcgagtcgt tcaagcagtc gttcaatctc
    13021 ctcatctgaa agaaaggcca tctctgtgtt cctgactttc agcttacgta ttccccgaac
    13081 cgggttttcg ttatggaaca cctccccctc aatcagcgag ctgaacattg cagacaaggc
    13141 acacaaatcg cggttaatgc tggaaggcat taacccggcc tgtaatttgt cggaccggta
    13201 cccgagcata aattttcgcg tcatctgact ggcacgagga tcacccatct cacggtttat
    13261 cttttccagt ctgaccctgt aggtatcccc gtaatcctgg ttacgaccat caagcagcca
    13321 ccaggcctct atcagttcgg ataaccgacg ctgatctgct ggcttagcct gccatggctt
    13381 atcatgaaaa ttctgcaaaa catacctttc aaactcctgc gcctttgact tcagggtaaa
    13441 aatcttacga atccgctttc cttccgtccc ttgtggcctt acatcaacct tataacgtcc
    13501 gtctttgagt tgcttaatcg acatgattag cccctccaac ggataaatta accaaacact
    13561 ccctgcggga tgaaacccgc tcgatgtgta acgtcagcca gcattgcggc ctgatcggga
    13621 tgatttttgg gtaacgtcga ttgaagccag atcgccctcc gactgagaag atccatcaag
    13681 agagagagcc ggggcaattt gcccggcggc aggtagcgtc ttatccttta acagccaaaa
    13741 catatacttt tcaaatcgat ctgcctgaag aaccgactct acgattttag ctctggcgct
    13801 cttttgccct gattcataat tccttacggt ggccagagat aaccctgtaa tttcagcaaa
    13861 ttgtttttgc gttaagccct ctgccttacg tatctggcgt aatttttccg cgtagtctat
    13921 tgacaagata accatttgag tattaacctc tcgaaaaaga taacctttcg attatcttta
    13981 caacccaaaa caaaccttca taaggtgcaa taggcgcacc tgtgcgagag gataacaaat
    14041 gcgggacttc attgccgata aagagttatc tgacaccgat cacacaactg gagcggcgta
    14101 catcaacgcc gataccagtc aacaagaagc aacagataac aacgacgaac aaaagccaca
    14161 gcgcacatat aaaaaagggg caaccctgcg gctggatggc ccaatagccg ggctttgctc
    14221 acttgaaaaa ggggccgcct acatcggatt aacaaaaaca gcattacgcg cagctcttca
    14281 gcgtaaccag atgccagggc ataaaacccg ctcaaaccct gaagatgaga attcggatgg
    14341 tgtttggtgg tttaacgtca gagagtggga caagctggcc gacgaactcc cggaatgcga
    14401 gccaccagaa tggcacaact ggaaaagcta ctggacatac gaccgccaga aaagaaagtt
    14461 taccccggct gataaggaag attgccagac cgtcaatgga aaacgggttt acaaaggcag
    14521 aaagtcaaag ctggaacaga gccaggatgt aaacgaacgg gggtgaacga tgagcggcaa
    14581 aaataaatca gaaaaaaccg caaccgtcct taagcagtta agcctcctga aagaggaaca
    14641 caaagatgca acgatactga tgcatctgga aagctcaccg aaaataacaa tcacaacaac
    14701 ctatagcgag ttacacaaag agcttgaagg attaattaac ttttgttcaa agctaaaagc
    14761 gaatgaaaac attgatgacc tgatacttaa accagaagca gagttcgggc acttttgcga
    14821 aaatagagaa aatggatgca tcggaataat gctatacgca acccgtggaa taatgattaa
    14881 tctgcccgtt gagcaggctg tggatatatt cggagaaata gcacttaata aaaccactat
    14941 ttacaaaaga aaaatgccgg agtaacccgg catgttggtc ccttagaaaa gttccaggaa
    15001 ctcttgcgag ggtatttatg aagtactttt tcgcggacat cgttagccag atctctgatt
    15061 tgatcgtact tatcatcaga agacggagga acttagggga ttttatccac gatttcaatt
    15121 actgatttaa gcatttttat taattcatcg tcgtaagaca ttgctatctc ctttttctgg
    15181 ttgtgtggaa acaccagaat accacgcgcc gggcgtggtt aaacatcccg gcactgattt
    15241 tcatttaact taaacggggt tttccgatat gacacagaga cgcagcctcc gcactggcac
    15301 agaacgccac gcagcccgtt tctcaaccag cgcatcacgc agcaactcgc actacagcct
    15361 gcaggaaacg cacgccacac ctgacgggca tccggttaaa caaaccggta aacacacctg
    15421 gctgattaat gatgccggaa ttgtcattca ccggggcgca cgcaacccga ttaccggaaa
    15481 tacctgttac tccctgactc gcggagatga gcagttcg
//
LOCUS       DAZGTJ010000107        15073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000107 DAZGTJ010000000
VERSION     DAZGTJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.107"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            289..870
                     /gene="mdaB"
                     /locus_tag="ACHK8E_004246"
     CDS             289..870
                     /gene="mdaB"
                     /locus_tag="ACHK8E_004246"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP0346437.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            901..1215
                     /gene="ygiN"
                     /locus_tag="ACHK8E_004247"
     CDS             901..1215
                     /gene="ygiN"
                     /locus_tag="ACHK8E_004247"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP0346438.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1260..2147)
                     /locus_tag="ACHK8E_004248"
     CDS             complement(1260..2147)
                     /locus_tag="ACHK8E_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0346439.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2144..3091)
                     /locus_tag="ACHK8E_004249"
     CDS             complement(2144..3091)
                     /locus_tag="ACHK8E_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0346440.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3102..4139)
                     /locus_tag="ACHK8E_004250"
     CDS             complement(3102..4139)
                     /locus_tag="ACHK8E_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0346441.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4148..5131)
                     /locus_tag="ACHK8E_004251"
     CDS             complement(4148..5131)
                     /locus_tag="ACHK8E_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0346442.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5128..5937)
                     /locus_tag="ACHK8E_004252"
     CDS             complement(5128..5937)
                     /locus_tag="ACHK8E_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0346443.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            6311..8452
                     /locus_tag="ACHK8E_004253"
     CDS             6311..8452
                     /locus_tag="ACHK8E_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP0346444.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(8560..9891)
                     /locus_tag="ACHK8E_004254"
     CDS             complement(8560..9891)
                     /locus_tag="ACHK8E_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346445.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINNEYYEQIKVCLQPKNI"
     gene            10178..10930
                     /locus_tag="ACHK8E_004255"
     CDS             10178..10930
                     /locus_tag="ACHK8E_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0346446.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            10944..12020
                     /locus_tag="ACHK8E_004256"
     CDS             10944..12020
                     /locus_tag="ACHK8E_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0346447.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            12020..13018
                     /locus_tag="ACHK8E_004257"
     CDS             12020..13018
                     /locus_tag="ACHK8E_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP0346448.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            13023..13937
                     /locus_tag="ACHK8E_004258"
     CDS             13023..13937
                     /locus_tag="ACHK8E_004258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0346449.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            13965..>15073
                     /locus_tag="ACHK8E_004259"
     CDS             13965..>15073
                     /locus_tag="ACHK8E_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFP0346450.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDGHKSLIFVSDNNFQPHQQTKFYLFIDKE"
BASE COUNT         4426 a         3606 c         3340 g         3701 t
ORIGIN      
        1 gtgtttttcc tttaattgat aagtatatga tatttgacat atatattctc catgattatt
       61 tttccgaaaa actactttat catttacata atacatctct cctgtgggaa cttataggaa
      121 gttgatttca gtattgtcat tttcagaaaa tatttatccc gcatcggtga atcagagcaa
      181 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg
      241 ctctgtgacg tactatagtc gacaaacgtc tcaccttgag gttaaaaaat gagcaacatc
      301 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
      361 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc
      421 gagagcgact acgatgtcaa agccgaagta caaaactttc tctgggctga tgtggtgatc
      481 tggcagatgc ctggctggtg gatgggcgcg ccgtggacag tgaaaaaata cattgatgat
      541 gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg caaagatccg
      601 tcgaaaaaat acggttctgg cggtctggta caaggtaaaa aatatatgct ttccctgacc
      661 tggaacgcac caatggaagc tttcaccgaa aaagatcagt tcttccacgg ggttggcgtt
      721 gacggtgtgt atctgccgtt ccataaagca aaccaattcc ttggtatgga accgctgccg
      781 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
      841 cgcaagcatc ttgtggaaat ttttggttaa ctagagctct ggctttagaa ggagttaacc
      901 atgattacag taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcagtg
      961 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
     1021 gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc
     1081 gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac
     1141 atgaaagcat atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat tcgtatcctg
     1201 cagccggggc tttgacattt ctaaaaagag gccggtgctg ctaccggtct cagcccagat
     1261 tagccattct ttcttcccgt tttcagtcct cgatggaaat cgttaattcg cttaatggtt
     1321 ttaaccgcac gctgcggcac tgcggaagct accgaccaga caatcatttc cagacacaac
     1381 aataccgtac catgtagcgg cgttttccct ttttcatccc cgcgcggaac atgaatcacg
     1441 atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat aacagggatc
     1501 cccagccgcc tcgcctcacg aagcattgtc agcccttccc ggtgcgcaga tttttgcgcc
     1561 atcatgatca gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag gccaataccc
     1621 gtacggttta gtgccgtggc aggcaaacct atgcggttga atagtcgtgc ggtatagtcc
     1681 gccagaatac ccgaagcgcc aatgccaaaa atagcgactt gccgtgcctg caccaacaac
     1741 gctacagctt gcgcaagcgc gtggcggttt tcaggctctg acaggacatt acaggtatac
     1801 aaatgccctt caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa gttactcacc
     1861 gtcgtagaca tcttttcgct ggaactaagg gctggaccaa gccactgttc cagagtgcgt
     1921 ttcagatcac gcagcccggc aaaccccagg gcctgtatag ccctgataac tgtggcatcg
     1981 gaagtgttca acgtggccgc aatttccatc gccgtttgct cgagtaccac ttcacgatgc
     2041 tcattaatgt agctggcgac aactcttaac ccaggcgtta actgactcgc gcgagtgcga
     2101 aaacgctcac caaaaatatc aacccgcact ttatctttct ggatcattta gcctccggta
     2161 gaggtcgccc tgcaatttgt gattgtatct gcgcagccat cagccccaga gatgcaaagg
     2221 agttagaaat tgcctcttca cgagaaatca gcacataacg cccggaacga caggcagtca
     2281 gaaactgaca ccatcccggc atgacttttt ccattgctgc cagctcatcc tgaggtttac
     2341 caccggtgtc accgcgccag gttgcaaaaa caaaatcggc atccagttca ggtaaacgct
     2401 ccgcgctcac atccattcgc ccaccttcag gaatgctctc aatcagcggc ggaaaacgaa
     2461 aacccgcatc gcgtaatacc cgccccagcg aatgatagct atgcatcaca ttaatttttc
     2521 cctggtttgc ctgaatcacc gataccgtta ttttttggct gtccagcgtc gccttcagcg
     2581 cattgatttg tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc gttcctgtaa
     2641 gtcctgccag cttgcgatag atttctggcg cacccccttt gagatgatca atgctcaccg
     2701 tgggcgcaat tttttcaagt tgttcaatgg gcgtattacg ggtcggttct gtgatgatca
     2761 ggtcaggctt tgccgccaca atcgcttcaa tatcaatatc agcggtacca ataaaggcga
     2821 tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc agatcgaatg aaatggctac
     2881 cgtcaggccg agttcgcccg tggctggcga caggaggtac accgagctca atcagaggaa
     2941 tagtgatatc aagatcgtgt aaagagacaa ttctctttgg atgagcagga acagtgactt
     3001 tgcgccctaa atcatcagta aatacctgaa caggttctgt tgcacgggaa aaaaatgaca
     3061 acagtatcag cagagaaaaa aataaacgca tataactccc gttaaaaact gttcctgcgc
     3121 tgccagagca gtagcaaaaa aaatggtcca ccaatcagtg agataatgat tccggcaggc
     3181 agttgcaaag gcaaaaaagc caaacgacca atgctatccg ccagaataac taacaacgcc
     3241 cccgataccg cgctccccag cagtaatgtc gtctgcccgc cacgtagaat aaaacgtgat
     3301 atatggggtg ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac gcagacggct
     3361 gtcaggatta ttggtgcggc gacacgtaac aacgccagac gagaactatt cacccctaac
     3421 ccggttgcaa cctgatgacc aagcagagcg atatccgccg tcctggcggt gaacagcaat
     3481 aaaagagcgg ctggcaacat ccaacaggct gaaataccga ctaacatcca gttagctgca
     3541 tgcaggcttc ccgataacca catcaatgca gtttgtacat ctcgaacatc tgcagtggtc
     3601 ataaatacac cgataccggc ggcgaaaaac caggaaacac caataccaat cagaacaaat
     3661 cgcgggcggg aaatatcgcg cgcacaaaaa atcacgatta aagccaccaa caagccacca
     3721 gcaagaccag ccactggacg ccagaacatt cccagcattg gaaactggaa aatcagccac
     3781 agcaccgcca cgctacatcc ttctttgacg ccgattaaac caggatcggc aaggccgtta
     3841 cgcgcaatgg attgcatcgc ggcccccgcc atacctaaca tggctccgca tagcaatgcc
     3901 atgatgactc tcggcaagcg aatatcctgc acaatataac gtgcgtctgt gcttacgttt
     3961 tctggagcaa acaacgctcg tcctacttcc gatgccggga cagaaaaaga accatgcatc
     4021 agaccgaata tcagtattcc aaccgcaaac agaaacagcc caccaagcaa caccagattt
     4081 ttagggcgta ccagtgtcga aaacttacca acacgtagtg ggcgtagccc gacgttcatt
     4141 atccgactca tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca ccaactaacg
     4201 ccgtcatgac accggtcgct aactcatggg gcgtgaaaag cgtccgagca ataatatctg
     4261 ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg cgcagatccg
     4321 ctgataccag acgccggaca atctgcggta ctagcaggcc gacaaaacca atggggccag
     4381 caatcgatac ggctgcgcca cagagcaacg cgatcgcaag cagggtgaat gttcgcgtgc
     4441 ggagtaccga gacgcccagc ccctgcgcca tgcgatcgcc cagtgccaac atgttaagag
     4501 agggcgcaag gtaaatggct aacacaaatc cccccagaga aaaccaggcg gacgtaccca
     4561 gcgtcaccca atcctgcccc gctaaatcac ctgctagcca tgtccgcatc gccagcagtg
     4621 tttgttcgtc aaaaatcagg attgcagccg ttattgagga cacgaacgcc gatagcgcca
     4681 cgccgcataa agtaactttc attggcgtta accctgaacg ccctgcggag gaaagcagca
     4741 atatcaacag gaacaataac gcgccccccg tagatgccag cagtgggcgt ccaaccggaa
     4801 aggccagtcc aagcgcactg gcggcaacga cagcaagagc ggctccggca tttaagccta
     4861 aaatatgtgg ctcccccaat gggttacgaa tgacagcctg caacaaagca ccagcaacac
     4921 caagagaagc accggtaagc aatgctgccg ctaaacgcgg caaacggagt cttacaatta
     4981 cgttgtgatc gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc gtttgtggtg
     5041 caataaatcg ggcaccaatc cctggatata ccacgcatcc caccagcagt aaggcgagga
     5101 aaattacagt aaccagccct gtgcgcatca tactttttct acaccacgaa acggcatgaa
     5161 gaacggcttt cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa agacagcttt
     5221 taccagttca ggcgtgcaat gctccccctc attcaacaca cgcaccactt tcccctggcg
     5281 taaaaatatc aacgtatcgc cataattgac ggcaaaattc agatcatgca acaccacgac
     5341 gactgtacgc ccgtgatgac gcgttaaatc atgtaataac tcaagaattt caacctgata
     5401 acgtaaatca agccaggttg tgggttcatc cagcaggata taaggcgtct tttgcgccaa
     5461 caccatcgca atccagcaac gctggcgctg cccaccagag agtttttcaa caggtaaatg
     5521 tgcaaactcc tgcgtaccgg ttaatttcag agcctcttct accgcctgct cgtcggcgtc
     5581 gctccattga cgaatgaaat tctgccaggg aaaacgccca cgcgacacca gttcataaac
     5641 ggttaagccc tccggcaata atggcgactg cggcaagatc ccaagtctac gggataacgc
     5701 tttcgttggt tgctcgtgaa tcgctttgcc atcaagcaat atcgaaccgc ccataggttg
     5761 caagatacgt gcaatggtgc tgagcaacgt cgactttcca cagccgtttg ccccaaccag
     5821 aacggtcatt ttttctgtgg gaacggagaa agaaacacca tcaacaatga tcttcttgtg
     5881 atacccggca gaaacattgt cgaggatcag cccctgccct tttgcgttat gtgccacgtg
     5941 tacgccaact taatcaaaga caaacaaaaa taaaactcat tctcctttga gagtttgacg
     6001 ccacaaaatg tagtagtcaa gcagttaaaa gccacacaaa aacaaggtaa ataacaggta
     6061 aactattttt atacataaaa ataaaaacat taaatatcaa taaattaaaa gcaatcaaat
     6121 tgtataaact tttcataaaa acacatgtag caattttttg ttatttctca tttactacta
     6181 cattactgcg tgattgaatg attctcaatt acatatccga tggcgaaata acaaacggat
     6241 agtgcttagt gagcaataag aaacaatttt cactcgcgac aatccgcgag tttttgggat
     6301 aacagactaa atggctatgt tcacaccttc attctcagga ctcaaaggtc gggcgctctt
     6361 ttcactgctt tttgcggcac cgatgattca tgcaacagac tctgtaacga ccaaagatgg
     6421 cgaaacaatc actgttacag cagatgcaaa taccgcaact gaggcgaccg atggttatca
     6481 acctctgagc acttccacgg cgacattaac cgatatgccg atgctggata tcccgcaggt
     6541 ggtcaatacg gttagcgatc aggttctgga aaaccagaat gcgacaacgc tggatgaggc
     6601 gctttataac gtcagtaacg tggtacagac caatacatta ggcgggactc aggatgcctt
     6661 tgtacgccgt gggtttggcg caaaccggga tggctccatc atgaccaacg gtctgcgaac
     6721 cgtacttcct cgcagtttca acgccgccac agaacgtgtg gaagtgctaa aaggcccggc
     6781 ctccacgctg tatggcattc tcgatcctgg cggactgatt aacgtcgtga ccaagcgccc
     6841 ggaaaaaaca ttccatggtt cggtttcagc cacctcctcc agttttggtg gcggcactgg
     6901 gcaacttgat atcacaggtc ccattgaagg cactcagctg gcgtatcgcc ttaccgggga
     6961 agtgcaggat gaagattact ggcgaaactt cggtaaagag cgcagtacat ttattgcccc
     7021 gtcactcacc tggtttggtg ataatgcaac agtaaccatg ctctattccc atcgggacta
     7081 taaaactccg ttcgatcgtg gaacgatttt cgaccttacg acgaaacagc ccgtaaacgt
     7141 tgatcgaaaa atacgttttg acgaaccgtt taatattaca gatggtcagt ccgatctggc
     7201 gcaactcaac gcagaatatc atctcaatag ccagtggaca gcgcgctttg attacagcta
     7261 cagccaggat aaatacagcg ataatcaggc gcgtgttacc gcgtatgatg caacgacagg
     7321 aacactgaca cggcgtgttg atgcaactca gggatctacc cagcgtatgc atgctactcg
     7381 tgcggatctg caagggaatg ttgatattgc cggattctat aatgagattc tgggtggggt
     7441 gtcatatgaa tattatgatc ttctgcgtac agatatgatt cgctgtaaaa aagctaaaga
     7501 tttcaatatc tacaaccctg tttatggtaa taccagcaaa tgtacaacgg tttcggcgtc
     7561 ggacagtgat cagacgatca aacaggagag ctactcagcg tatgcacagg acgcactcta
     7621 tctgaccgat aactggattg ccgtcgccgg gatccgctat cagtattaca cgcagtatgc
     7681 gggtaaaggc cgtcctttta atgtcaatac tgacagccgc gatgaacaat ggactcccaa
     7741 actggggttg gtctacaaac tgacgccatc ggtatcctta tttgccaatt attcgcaaac
     7801 atttatgccg cagtcgtcaa ttgccagcta cattggcgat cttccaccgg aatcatctaa
     7861 tgcttacgaa gtcggggcaa aattcgagct attcgatggt atcaccgcag atattgcgct
     7921 gtttgatatc cataaacgta atgtgttgta taccgaaagt gttggtgatg aaaccattgc
     7981 caaaacggca ggccgtgttc gttcaagagg ggtagaagtc gaccttgcgg gagcattaac
     8041 tgaaaacatt aatatcattg ccagctacgg ctataccgat gctaaggttc tggaagatcc
     8101 tgattatgca ggtaaaccat tgccgaatgt tcctcgtcat accggttcgc tattcctgac
     8161 ctatgacatt cataacatgc ctggcaataa cacactgacg tttggcggtg gtggacatgg
     8221 cgtaagccgt cgttcggcaa ccaatggggc tgactattat ctgccaggct attttgttgc
     8281 cgatgccttc gccgcataca aaatgaaatt gcagtatccg gtcacactgc aattaaacgt
     8341 caaaaacctg tttgataaaa cgtattacac ctcttccatc gccacaaata atctggggaa
     8401 tcagattggc gatccgcgtg aagtgcaatt cacggtgaaa atggaatttt gatcaaaaaa
     8461 gcccggtatg tttaccgggc tgagactgat gacaaactcc aaattgtctg atgcgctacg
     8521 cccatcagat ctaccataga aattcaatat attaaatttt catatatttt taggctgtaa
     8581 acacacctta atctgttcgt aatattcatt attaatcaga taaaatctca tgatgagtcc
     8641 ggtaatcagg ttaagtatcg cgggaatgat cgtcattaac aggcagatcc cccacaacgc
     8701 ctgtggtgtt tgttctacgc caggttgata gtccataaac gctaaagcga ttccgataaa
     8761 ccctcccgcc agtcccatac caaacttttg aaaaaagaga atcccgccaa atgccagtcc
     8821 agaaacacgt tgccccgttt tcatttcacc ataatccact gactcagcaa tggatgccca
     8881 ataaatggga agctgaatat cagtcagaaa ggtcaccaca atataaaatg taaaagctaa
     8941 cactatagaa tttggttgca cgaaatacat cgcaccaccc accacgaacg ccagtaattg
     9001 ggtataacga aatattttta ttttgtcgta gagtcgagta agccaggtgc aagcgatcat
     9061 agccagaaca gaggcgatca cgcctgaagt taaaaacggt gaaattaaag catccccacc
     9121 attcaggtaa tattttgcat aatatgcagc gacagagtta cgaataatcc cgccaaacat
     9181 aatgatcgca attacagcgc ccaaaatgag ccattgatca tttttgatca gtaaccgaaa
     9241 ctgcgcggcc ataccgagac tgggttttgg atggtagatg cgctctctca cgctaaaaaa
     9301 acaatacagg cataaacacg ttgccagaat tcccattgcc cccattgcta tttgataacc
     9361 cagttgtacg ttattcttcc caagatacat tgcagataaa ggaacaataa ttgttacggc
     9421 gaacatggca atttttgtca taacaaaacg ataaccattg gcgctcaatc gctcttgtgg
     9481 atcatccgtg ataacgctga ttaatgaaac ataaggaata gcgattaacg tataggtcaa
     9541 ggtaagtaac acataggtag accaggccca taaaagctta actgaataat cggtattagg
     9601 tgtggtgaac atcaaccaaa tgcttatccc aaacggtatt gacataaaaa gcaaccacgg
     9661 ccgatatttt ccccaacgtg tattcgttct gtctgtcatt gttcccacaa ctggatcaat
     9721 cacagcatca aggatacgta cagaaaataa cagaatacct aagtcgacgg gtttcagacc
     9781 aaatacatcc gtgtaaaaat aagtgatgat catggccaaa gacatcatta ctatcgcgat
     9841 agccatatca ccagcaccaa aacctatctt ttcatttatt gtaagtttca tacacagcac
     9901 tcattgatta cgtgaactta tattaaaaac aaatatatct gaactcattc agcacataac
     9961 tttacgagtg agcataaatg cattggttga taccacaaca aaccattgag tcaatattga
    10021 gcaacatcac ataaaaacca atttcactta atctcatgat ttatatattt aattgtggtt
    10081 ttttctgttt tatgtcacag atagtgaaca agcaccatgc gtaataaaag ttaaagaatg
    10141 tagagttggt atcaaccact tgaaatgagg ctttgtcatg ataaaaattt ataaagccag
    10201 taaaggtcag ctaaagcatc gtgaaatctt tgatgaaata aagaaaaaag tagataacgg
    10261 tgaatggaaa gaaggcaaag ccataccgac agaaagggaa ttgtcagaat attatttaag
    10321 tagcagaaca actattcgaa aggcaataga aagtcttaaa caacgcggct acctgcatag
    10381 tgtccacggg caaggaacat ttgtattgcc ttctcgtagt agagaaaatc atttattgca
    10441 tagttttacc gacgatatta aagctcgagg tggtatacca gcacagaata ttcttgagat
    10501 aggctatgtt cctgtcagtg atgttatcag aaaaaatctc gacctggata tccatgtcca
    10561 aaccgtattc tgtataaaac gcgttcgcta tatgggaagt acccccctgg gtattcagac
    10621 atcatggctt gcattaaatg acaggcaaga aataacccaa gaagatctgc tggcgacggg
    10681 ttcgctctat attttactgg aagaaaaact cgctattaaa cctttagaag caacagaaat
    10741 tatctctgcc cggcttccct cgccccttga gcgtcaatta ctggaactga ctgccgatga
    10801 tgtcgtatta agttgtaccc gcgtcacgtt atccgtggaa cgcaaaccaa tggagtatgt
    10861 agagatggtg tatcccgcaa gccggtactc ctacaaagca cgaattacaa aagacagttt
    10921 tagcgtgtaa cagagagaat attgtgaaca aaatagaaca agctgtaatt tataacaaaa
    10981 tattaggttc tttagcctgc gcaggtatgg gcgatgcatt aggcgcagcg actgaactct
    11041 acagcattga tgaaataaaa gctcaatggg gaggtttttt aaatgccttt gtttctcctc
    11101 ctgccgatac attcgctggc tcattaaatg gtattgcggg acttatcacc gacgattcca
    11161 gccaaatgta tgtgttttca gaagggttaa ttgaggctgg atttgataac ttcaccaata
    11221 atgactggtt agcttgtctt ctgcgttggg ccgatatgca gccctacgca aattataaag
    11281 gccccacaac agaacaaatc gtcaaggcac taaaagaagg tagaccaaca aacaccattg
    11341 ggcgtattgg aacatcttct cgtcaggcac ccaatgtcgg caccacgaat ggagcaggaa
    11401 tgcgtgtagc ccctgcagga ttgatctggt caggcaagaa agaaaaagcg tgtcacctgg
    11461 cgctaataac ttgcctacct tcgcatgaca ccaatattgc gattgccagc gcttgcgcca
    11521 tcgctgcggc gacatcacaa gctatgttac cagaagcttc gctcacatca ttactggacg
    11581 ccgcaatctg gggagcgaat tacggcgaac gcctggccaa acaatatgca cgatgtgtcg
    11641 cagggccatc tatcgccatg cgcatccaac ttgcagcgga catcgccaga cgagccaatg
    11701 acctggagtc ttgtctgcga gaaatggaag gtttggttgg taactctgtt gccgcccatg
    11761 aatccattcc tgcggcaatc ggtctattgc tgtactgcaa aggagaaccc tgggaaacca
    11821 ttcatgcctg cgccaatatt ggcaatgaca ccgatagcat cgctacgatg gcaggcgcaa
    11881 ttgcaggggc atggcgcggt tttgatgcgc tccctgaaga taaatatgcc tttttccgcg
    11941 ccgtaaataa taaagatttc gatattgaag cgatcgcatc gggtctgaca ttgcttgcat
    12001 tagaggcaca ggagaaataa tgaacatgcc agcccgtact cctgttatgc cattagcgca
    12061 aactcgtgag gagataaatg ctacggcggc atcgttacgc catttggcag ctatctgggg
    12121 tgaatgtttt tctacttttc agctcgataa acgtgctgtt gaacgcgtct gtatagtggg
    12181 atcgggcgac tcctggacgg tggcactatg tgccgccgcc tggctgggga aatacacaca
    12241 tctgttttgt tacgcattgc aaacgtggga ttttcttcag actgatttaa cccgttatca
    12301 gaaagaaacg ttaatcatta ttctcagtgc aagtggcaga ccatcaatga ccgttgacgc
    12361 cctctgccac gcagtatgct caaacgcgca agtgctgggt gtgacgaatt gccctggaac
    12421 accattttgc gcaataacag aaaacatgct ctatacatgg gcgaataagc agggtattcc
    12481 aacgcaaagt agtagcgtca cactttatgc cctcctgcgc ttagcgcaaa aattatgtcc
    12541 aggcttaaca ccactgcaga ttgaagatga tttggaaggg aaatttagcc agattaatca
    12601 gcactggcag caaaagaagc gccattttta ccaacaaaaa gagataacgt ttcttgggag
    12661 tggcttaagt tggggactgg caatatcggg gagcaattta ctttcttgcg gcccacagat
    12721 tcgtgcgaca gcccttccac tggaagaatt ttatcattca ttacgcttac atcaggctgg
    12781 tgtcggtcag cattatattt tactaccagc cacatcctgc gagtccccat tttaccttgc
    12841 aacccaaaaa gcaattgttg aacagggagc gacagcagaa ttaatcagtt ttatacctga
    12901 cgccagtgag tccaacaatt tatttctggt catgcagtgg ttatatgaaa tgtgctggca
    12961 tttaagttgt gattatgtcg acgcaggcgg gcaacgcgtt agccataggg agaaataaga
    13021 ctatgaagaa atttgatgtc gccgctttag gcagtggaaa tatagatatt tttttatcta
    13081 tcccgtcact ccccactcgt ggcggtaaag ttgtcgggac acgtctgggt gaacaagctg
    13141 gcggcacagt cgccaacagt gcctgtgcga tgggacaact gggtctcaat gtggtatctg
    13201 tttcatgtgt gggcaatgat cattcagcct cgatcattct tgatggattt aaaaaatatc
    13261 atgtaaattg tgatttcgtt caggtaattc cggaattgat tgccaatacc gcgattatct
    13321 ttattgatga attaggtgaa aaaacgttgg tttactcgcc tggtagtgat catgagtggg
    13381 ataaagaaaa agccctgcag gcaattgcgc aaagccgtta tttttatacg atgccagcag
    13441 atatcgagaa atttcgtatg ctggccgaat attcccgtag ccagatgaca aaagttgtcg
    13501 ttgatattga gcctcatatc attgcaacac ctgaacacct tgccagaatt ttacaccttg
    13561 ctgatattgc gatatttaat tatgacggtt ttattcgtgg ctatgcaaca gagccagatt
    13621 ttacacttct gcatgatata caagacgagt atcaacttga cgctgtcgtg gttacgctgg
    13681 atgttagggg agtaattgca gtcaaaggca atgagcaagt aaaaataggt agttatacaa
    13741 ttcccgttat tgataccaca ggcgctggag atacttttaa tggtgcattt gtttatagct
    13801 taataaaaaa tatgccctta attgaagcac taaaatttgc ttctgccaca gcagcaataa
    13861 atattactgc attaggtgcc agaggtcatc tgccgacacc atcggaggtt aatctttttc
    13921 tttcacaaca cgactaaaat catatatata caaaggtaaa tatcatgaaa ataagatatg
    13981 ccgctatttt tctggcatta ccgctgttat gcacaacagc gcaggcaaac agttttcttc
    14041 attatgctgg ggagtttaac ttccggacgg gagaaaagca gaacaatgtc acaatcgcgg
    14101 ggctatcagc catcacattt gatgcaaaaa aaaatatttt ctatgctatt aacgatagca
    14161 gaaacaataa taaagaagga gatgccagtc tttatacact aaaaatacag gtatcatcca
    14221 ggggcattga gcaagtgaat tttctcaacc agcgtccact gcttgatgca aaacagcaac
    14281 ctttcgtgac caatacggtg gatgccgaag gtctggccct gacacacaat ggtaaatcat
    14341 tactctggag ttcggagtta ggtgctccac tgcgtctgag cactctcgat ggcgtaatgg
    14401 agaaagattt tacgtcttta tttcctgctc gctttaatat tagttctgac aaagagagca
    14461 gcaatggtat tcgcagcggc aacgcctggg agggcctgac agttacacca gatggaaaat
    14521 cactttttat cgccgttgaa agcagcctta aacaggatgg gcctattgca agtccaataa
    14581 attcaggcac atcacgctta ctgcaatttt ctatagatgc agacggtcga ccttcaaagc
    14641 aattgcatga atatctctat attactgatc ctgttccgca agtgagtaag ttcggtatta
    14701 atgataatgg cgtatccgag gtcatggcgc tgaatgatca ccaattgttg gtcatcgaac
    14761 gttccggcag aaacgtcagt gctgggttca atgactggga ttactctgtc agagtttata
    14821 tggtcgattt aactgctgcc agcgatatca agaatattga cagtctgcaa gattggtcca
    14881 ataaatccac gttacaaccc gtcagtaaaa aattattaat tgattttgct gattacactt
    14941 cctctgcgga ttgtattgaa ggggtaacct tcggtcctct gattgatggg cataaatcgt
    15001 taatatttgt atctgataat aatttccagc cccatcaaca gacgaaattt tatctattca
    15061 ttgataagga aaa
//
LOCUS       DAZGTJ010000108        14960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000108 DAZGTJ010000000
VERSION     DAZGTJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.108"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(177..884)
                     /gene="yqgA"
                     /locus_tag="ACHK8E_004260"
     CDS             complement(177..884)
                     /gene="yqgA"
                     /locus_tag="ACHK8E_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP0346451.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1037..1114
                     /locus_tag="ACHK8E_004261"
     CDS             1037..1114
                     /locus_tag="ACHK8E_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346452.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1277..3412
                     /gene="speC"
                     /locus_tag="ACHK8E_004262"
     CDS             1277..3412
                     /gene="speC"
                     /locus_tag="ACHK8E_004262"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP0346453.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3462..4718)
                     /gene="nupG"
                     /locus_tag="ACHK8E_004263"
     CDS             complement(3462..4718)
                     /gene="nupG"
                     /locus_tag="ACHK8E_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP0346454.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4920..5999)
                     /gene="mltC"
                     /locus_tag="ACHK8E_004264"
     CDS             complement(4920..5999)
                     /gene="mltC"
                     /locus_tag="ACHK8E_004264"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP0346455.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6064..6339)
                     /gene="yggX"
                     /locus_tag="ACHK8E_004265"
     CDS             complement(6064..6339)
                     /gene="yggX"
                     /locus_tag="ACHK8E_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP0346456.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6367..7419)
                     /gene="mutY"
                     /locus_tag="ACHK8E_004266"
     CDS             complement(6367..7419)
                     /gene="mutY"
                     /locus_tag="ACHK8E_004266"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP0346457.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7580..8299
                     /gene="trmB"
                     /locus_tag="ACHK8E_004267"
     CDS             7580..8299
                     /gene="trmB"
                     /locus_tag="ACHK8E_004267"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP0346458.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8299..8625
                     /gene="yggL"
                     /locus_tag="ACHK8E_004268"
     CDS             8299..8625
                     /gene="yggL"
                     /locus_tag="ACHK8E_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP0346459.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            8809..9528
                     /gene="yggN"
                     /locus_tag="ACHK8E_004269"
     CDS             8809..9528
                     /gene="yggN"
                     /locus_tag="ACHK8E_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP0346460.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9704..10750
                     /gene="ansB"
                     /locus_tag="ACHK8E_004270"
     CDS             9704..10750
                     /gene="ansB"
                     /locus_tag="ACHK8E_004270"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP0346461.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10867..11874
                     /gene="yggM"
                     /locus_tag="ACHK8E_004271"
     CDS             10867..11874
                     /gene="yggM"
                     /locus_tag="ACHK8E_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP0346462.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(11930..13231)
                     /locus_tag="ACHK8E_004272"
     CDS             complement(11930..13231)
                     /locus_tag="ACHK8E_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP0346463.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13231..13734)
                     /locus_tag="ACHK8E_004273"
     CDS             complement(13231..13734)
                     /locus_tag="ACHK8E_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP0346464.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(13779..14765)
                     /locus_tag="ACHK8E_004274"
                     /pseudo
     CDS             complement(13779..14765)
                     /locus_tag="ACHK8E_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         3957 a         3582 c         3793 g         3628 t
ORIGIN      
        1 cccggaggcg gaatcgaacc accgacacgg ggattttcaa tcccctgctc taccgactga
       61 gctatccggg caacggggcg cattaaacct gattcgcctc gtctcgtcaa tcaaatttct
      121 tcaatttact gcagactgca caatctatca tcactttgcc gctattgcac gcattgtcag
      181 gcaaaccagg cggtccaggc ggcggaaagc ggcattgcca gaaggagtgc tggaagcatg
      241 ttgaccaccg ggaacatttt aatgccacag atgcgtaatc cggttgccag cagcaataaa
      301 ccgcctacgg cgctgaagtc tgccatcatc gacggtgtgg tcagcggtaa tatcagcgcg
      361 gcagcccacg ccagcgttaa ctggatgatc agtaatggga tactaattac cgacaccgca
      421 atacccaacg agcaggcgaa gatcatcgcc gtaaagaaat cgagaaatga cttggcgatt
      481 aaaatactcg gatcgccggt catcccttcg ttcatcgccc cgaagatccc ggtgccgctg
      541 gcgcaaaaca ggacaataat cgcgacataa ttctgaataa aagattcatg cgctggcttc
      601 ttacgtgagt ggcgaaacag attttgtgct ttggtgaccg cggtattgac gcctttttcc
      661 agcagacaaa tttcgccgat taatgcaccg agtaaggttg ctaaaaccat cgccggaagg
      721 ttggcgcatt tcaccaccag taaaatacca atccccagcg atgccagacc aaaaatagat
      781 gtcatggaga cgcggatacg ttccggtaaa cgttggctga gcagtgcgcc gagaacgcca
      841 cccagtaaga ctgcactagc gttgataaaa gggccgatga ccacaagagt tcctgttaac
      901 tgtattgtct gattttctta ttatgaactt tttgccttgt tgatggcttg tctggtcgca
      961 aagtcctggc ttgcacgctt tagcgaaagg tgccatgatt gcgcgaattt tctcctctct
     1021 gtacggagtt tgcccgatgc acgccacctc cttacattct ctcgcttatc gccgtttcgc
     1081 gcgaaactcc tccctttttc tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca
     1141 acagtaaaat cagagcgttt ctgcttttac tgatgtctgg cggtcggagc tggtgaccag
     1201 tttgacccac atctcatggg gcaggtttgt ccatcctgtc cggtactctt acttccccga
     1261 aacgggtttt gcgcttatga aatcaatgaa tattgccgcc agtagtgaac tggtatcccg
     1321 actttctact catcgccgcg tggtggcgtt gggagatact gattttacgg acgtcgcggc
     1381 agtcgtcatt accgctgcgg atagtcgcag tggcattctt gcgttgctta agcgcaccgg
     1441 ttttcatcta ccggtgtttt tgtattccga acatgttgtt gaattacctg cgggcgttac
     1501 ggcggtaatc aacggcaacg agcagcagtg gctggagctg gaatccgcag cctgtcagta
     1561 tgaagagaat ttgctgccac cgttttatga cacgctgacg cagtacgttg agatgggcaa
     1621 cagcaccttt gcttgccctg gacatcaaca tggtgcgttt tttaaaaagc atcctgccgg
     1681 acgccatttt tacgatttct ttggtgagaa cgtctttcgc gccgatatgt gtaacgctga
     1741 cgtaaaattg ggcgatctgc ttattcatga aggttcggcg aaggatgcac agaaattcgc
     1801 agccaaagtc tttcatgccg ataaaaccta ttttgtgctg aacggcacat cggcagcgaa
     1861 taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg gtgctcttcg accgtaacaa
     1921 ccataagtcg aatcatcatg gcgcgttgat tcaggcgggg gcgacgccgg tctatctgga
     1981 agcttcacgc aacccgtttg gtttcattgg tggtattgat gcgcactgtt ttaatgaaga
     2041 gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa gcagacctgc cgcgtccgtt
     2101 tcgtctggcg attattcagc tgggaaccta tgacggcact gtctataacg cccgtcaggt
     2161 gatcgatacc gttgggcatc tgtgtgatta cattctgttt gattccgcgt gggtcggtta
     2221 cgaacagttt atcccgatga tggcggatag ctcgccgctg ctgttagaac ttaacgaaaa
     2281 cgatccgggg atctttgtga cgcaatcggt gcataaacag caggcgggat tctcacagac
     2341 gtcgcagatc cataaaaaag ataaccatat tcgcgggcag gcgcgttttt gcccgcataa
     2401 gcggttaaat aatgccttta tgctccatgc ttctaccagc ccgttctatc cgctgtttgc
     2461 cgcgctggac gttaacgcca aaattcatga aggggagagt gggcgtcggc tgtgggcgga
     2521 gtgcgttgcg ttggggattg aagcccggaa ggcgattctt gcgcgctgta agctgttccg
     2581 cccgtttatt ccacccgttg ttgatggcaa attgtggcag gattatccga cgtcagtgtt
     2641 agccagcgac cgtcgttttt tcagttttga gccgggggcg aagtggcacg gctttgaagg
     2701 atatgccgcg gagcagtatt ttgtcgatcc gtgcaagctg ttactgacca caccaggtat
     2761 tgatgctgaa accggtgaat atagcgactt tggcgttccg gcgacgattc tggcgcatta
     2821 tctgcgtgag aacggtattg tgccggagaa gtgcgatctc aactccattc tgttcttatt
     2881 aacgccggcg gaaagccacg agaagctggc acaactggtg gcgatgctgg cgcagtttga
     2941 acagcatatt gaggatgact cgccgctggc ggaggtgttg ccgagcgttt ataacaaata
     3001 tccggtgcgc tatcgcgact acactttgcg ccagttgtgt caggagatgc acgatctgta
     3061 tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc cgccagcaga gtttcccgtc
     3121 agtggtgatg aacccccagg atgcgcatag cgcttatatt cgcggtgaag tggagttggt
     3181 gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg gcgttgcctt atccaccagg
     3241 cgtgctttgc gtggtacccg gagaagtctg gggtggggcg gttcagcgtt atttccttgc
     3301 gctggaagaa ggggtgaatt tattgccggg attttcgccg gagctgcaag gtgtttatag
     3361 cgaaaccgat gcggatggca tgaaacggtt gttcggttat gtgttgaagt aagaataaaa
     3421 aaaacgggtc accttctggc gacccgtttt tctttgcgta attagtggct aaccgtctgt
     3481 gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg ccatgaacgc gaaggccaga
     3541 accacggagt aaccagcgaa aatcagccat acggtctgcc agtcggtaat gccgttttgg
     3601 gtgtacatct caacaacttt accgctcacg atgccgccga ggatacagcc gaagccgtta
     3661 gtcatcatca ggaacatccc ctgcgcactg gcgcgaattg ccgggctaac ttctttttcg
     3721 acaaacaccg aaccagagat gttgaagaag tcgaatgcgc aaccgtaaac gatcatcgac
     3781 agtaccagca gtacagtacc gaacggagtc gggtcgccgt aagcaaacag cgcaaaacgc
     3841 aggatccacg ccacaatact gatcatcatt acgttcttaa taccgtagcg gcttaagaag
     3901 aacgggatgg tcagaatgaa cagggtttca gagatctgcg aaatcgacat gatgattgac
     3961 gcatgctgca cgatgaagct gctggcaaac atcggatctt tgtcgaagct gtgcaggaag
     4021 gtgttaccga acatgttggt aatctgcagt tccgcgccca gcagcattga gaagatgaag
     4081 aagattgcca tacgcttgtt tttaaacagc gcgaatgcat cgaggcccag cagggttgtc
     4141 cagctctgat tcgcttgctg tttagcaacc ggaatatgcg gcagagtcag ggtaaacaga
     4201 accagaatgg cggaaagtgc tgcgccaata tacagctgca tgtggcttaa ttcgaagcca
     4261 gacaggctca ccacccacat tgccatgata aagccgatgg tgccccagat acggattggc
     4321 gggaagtcag taacgatatc catcccggca ttttgcaggc gatagtaaga gatggtgttg
     4381 attaacccaa gcgttggcat ataagcaaac gagttaatca atatcacaag gaacatcgct
     4441 tccggcgtcg tgacctgtgc cgccatgaac agcgtgatag cgccaatggt gtggcaaatg
     4501 gcatataccc atttcgcact taaccatttg tcggccacaa tccccagcag cgcaggcata
     4561 aataccgcgg cgatacccag tgagctataa actgcgccaa tagaagcacc gtcaaacttc
     4621 agggtaacaa acatatagga gccgagggtc gtcagccaac ttccccacag acagaactgc
     4681 agaaaagaga ggattttcag ctgcagctta agattcatgt taatttcctc acatcgtgat
     4741 gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga tgatgggatt ctatcaactt
     4801 cccggactgg ttttttgtta cctgtggcaa ataattgcaa acattcccaa tttgcaaaac
     4861 gaaattgtga tgtggataac atttttaccc ctgagcatcg tcaggggcgg ttagtggaat
     4921 tatcggcggc ggtaggattt ttgcgcggta ttcactttat aaagataacg gcgagattcc
     4981 gcagagggat ggcgggtcgt cagcgtttga taaacatcgc ccggcgtcat ggtgttaata
     5041 atattggccg cctgaatctt atcattcgaa aagactcgca gcacgctgcc tgcgccgccg
     5101 ttataggcgg tgatgacggc ataacgccgg gatgtagggt tatcaattcc gccgagataa
     5161 acgttgttca gcatcgccag atacgcggtg ccggtatcaa tattgctggc aggatcaaac
     5221 aagaaactgc ggctcggcgt gccggatttc ccctgcgaac ggaacacatc tttcccggca
     5281 gtatgttgta ccacctgcat taaccccagc gcatcggaac ggctgaccgc atacgggtta
     5341 aaggatgact cggtctgcat aatcgccaga atcagcgact catcaacgcc atatttccgt
     5401 gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt tatcaaggtg gttcggcacc
     5461 atgttaatgg tgacgctgta gatgatacgc agcccgttgc tgcggctctt cagacggttt
     5521 ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc agcgaatcgg ctgcccggtg
     5581 ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa tcgtaatatc atcaacgtcg
     5641 gaatagagat cgaccgaact cggatcgtcc cccatcagca acgttttgat aattgcccgg
     5701 cgcagatgcg cggcaggttc tgtcccggcg atggtttcga tagtaatcgt accgtcatcg
     5761 aagttgatgt ggctgcgggt ctgatattga tcggtgtatt tcacgtagtc cttaggaccg
     5821 gcgatcacca cctctttgaa gccccagatg ttctcaatat tgtgggcaaa ttgccccatc
     5881 agaatatcaa aaccgttggt atctttgacc caggcttcgt tataggtatc gccttttttg
     5941 gtcgtcgaac aggagatgag caacggcgca atcaaagcca gcgcgagata ttttttcatc
     6001 attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc ggaggcgtgc tccggcactg
     6061 tttttatttt ttatcttccg gcgtatagcc ctcaatatgc acctctttac cctcgaacag
     6121 gaagttgacc atctcctgtt ccagcagctt gcggtgctcg gcattcatca tgttgagttt
     6181 cttttcatta atcagcatgg tttgtttgtg ctgccactgc gcccaggctt ctttggagat
     6241 ctcgttatag atgcgttttc ccagctcgcc ggggtacagc tgaaaatcct gaccttctgc
     6301 ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata aatcatcctc tttatcgact
     6361 cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac aaacgctcca cgggagccgc
     6421 caggccaact gacggcggtt gcgctaagtt ataccagagc gcattgcctt catccatgca
     6481 gccggtgaat gacgacacgg gaagccacat aggcacaata tctaagtgga aatggctgaa
     6541 ggtatgccga aacgcggtca gctgcgtcag gttatcggca gcaatctgcc gttgcgccag
     6601 ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa cagtataaac cgccccacaa
     6661 tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg tgctgtaaca ggagaaagta
     6721 gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc ggataaagcg accagctatt
     6781 gttggtggcg gcaatacatc cgttttgtag cggacagagc gagcatttcg gcttcgagcg
     6841 cgtgcaaatc atcgcaccca aatccatcat cgcctgatta aaccgctcca cgccgaccgc
     6901 gggcgtcacc tgttcgctca aactccacag tttattctcg acctcttttt tcccaggcca
     6961 gccgcttaca gcatagcagc gcgccagcac ccgtttgacg ttaccgtcga gaatcggaaa
     7021 gtgcttaccc agagaaagcg agagaattgc gcctgcggtg gaacgcccga cgcccggtaa
     7081 cgccgcgact tcttcaaagg tttccgggaa tttaccgccg tgtaaggtcg ccacttgttg
     7141 tgccgcctta tgcagattgc gcgcgcgggc gtaatagcca agcccggtcc acaagtggag
     7201 aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc gggaagcgcg ccataaagcg
     7261 ttcaaaatag gggataacgg tcgcaacctg agtttgttgc aacatcactt ctgagagcca
     7321 tactttgtag ggcgtcttgt caatttgcca gggcagcgtt ttccgcccgt atttatcgta
     7381 ccagtccaga acctgggctg aaaattgcga cgcttgcatg gtcaccgaat tcactgttgt
     7441 tgggggcaag attgcagcac agcgacggca gggtgtaaac cggaactttc cgcagccacg
     7501 gccttatcat gcttgcatcc ggcaactaac tttggataat gcccgttttc agaacacttt
     7561 cacaagcgac taaatcttta tgaaaaacga cgtcatctca ccggaatttg atgaaaacgg
     7621 ccgcccactg cgccgtatcc gtagttttgt gcgccgccag gggcgactga ccaaaggcca
     7681 ggaacatgcg ctggaaaact actggccggt gatgggcgtt gagttcagcg aagatatgct
     7741 ggatttcccc gcgctttttg gccgagaagc gccggtgacg cttgagattg gtttcggcat
     7801 gggggcgtcg ctggtggcaa tggctaaaga tcgccccgag caggacttcc tcggcattga
     7861 agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg catgaagagg gcttaagcaa
     7921 cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat aaaatgattc ctgacaattc
     7981 attgcgcatg gtgcagctct ttttccctga cccgtggcac aaagcgcgcc ataataaacg
     8041 ccgtatcgtt caggtgccgt ttgccgaact ggtaaaaagc aaactgcaac tggggggcgt
     8101 attccatatg gcgaccgact gggaacctta tgcggaacat atgcttgaag tgatgtcttc
     8161 tattgacggt tataaaaacc tgtcagagag caatgattac gtaccgcgtc cggcatcacg
     8221 tccggtgacg aaatttgaac aacgtggtca tcgtcttggt cacggagtat gggacttaat
     8281 gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc gtctgcgtaa aaaaatgcac
     8341 atcgacgaat tccaggaatt aggattttcg gtggcatggc gattcccgga aggtacatcg
     8401 gaagaacaga ttgataaaac cgttgatgac tttattaacg aggttatcga accgaacaaa
     8461 ctggcctttg acggcagcgg ttatctggcc tgggaaggtc tgatctgcat gcaggaaatc
     8521 ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt ggctggaaga gcgcaaactg
     8581 ggcgaggtac gcaccagcga acttttcgac gtttggtggg actaagaaag catacgggcg
     8641 atgacaaatg caaaactgcc tgatgtgcta cgcttatcag gcctataaag atgcacgatc
     8701 gagtaggctg gataaggcgt ttacgccgca tccggcatgg aaaacgcgca ctttgttatc
     8761 aatctggggc cagcaaatgc tggcctgatt tgttcttgag ggaagactat gatgcgcaaa
     8821 atgctgctgg cggcagcact ttcagtgacg gcaatgaccg ctcacgccga ctaccagtgc
     8881 agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa ccgtgcaggt gaagggcgaa
     8941 aacggcaatc tggtgatcac gccagacggc aacgtgatgt ataacggtaa gcaatattcc
     9001 ctgaatgccg cccagcgcga gcaggcgaag gattatcagg ctgaactccg tagcaccctg
     9061 ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag cccgtattgc gctggataaa
     9121 attatcgttc aggagatggg cgaaagcagc aaaatgcgca gccgtctgac caaacttgat
     9181 gcgcagctga aagagcagat gaaccgcatt atcgaaacgc gcagcgatgg cctgacgttt
     9241 cactataaag ccattgatca ggttcgcgcc gaaggccagc aattagtgaa tcaggcaatg
     9301 ggtggaattt tacaggacag cattaatgaa atgggcgcga aagcggtgct gaaaagcggc
     9361 ggtaacccat tacagaatgt gctgggaagc ctgggcggtc tgcaatcctc aatccaaacc
     9421 gagtggaaaa agcaggaaaa agatttccag cagtttggca aagatgtttg tagccgcgtt
     9481 gtgactctgg aagatagccg caaagccctg gtcgggaatt taaaataatc ctctatttta
     9541 agacggcata atactttttt atgccgttta attcttcgtt ttgttacctg cctctaactt
     9601 tgtagatctc caaaatatat tcacgttgta aattgtttaa cgtcaaattt cccatacaaa
     9661 gctaagggat aatgcgtagc gttcacgtaa ctggaggaat gaaatggagt ttttcaaaaa
     9721 gacggcactt gccgcactgg ttatgggttt tagtggtgca gcattggcat tacccaatat
     9781 caccatttta gcaactggcg ggaccattgc cggtggtggt gactccgcaa ccaaatctaa
     9841 ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat gcggtgccgc aactgaagga
     9901 cattgcgaac gttaaaggcg agcaggtagt gaatattggc tcccaggaca tgaacgatga
     9961 tgtctggctg acactggcga aaaaaattaa caccgactgc gataaaaccg acggcttcgt
    10021 cattacccac ggtaccgaca cgatggaaga aaccgcttac ttcctcgacc tgacggtgaa
    10081 atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg tccacgtcca tgagcgcaga
    10141 cggtccattc aacctgtata acgcggtagt gaccgcagct gataaagcct ccgctaatcg
    10201 tggcgtgctg gtggtaatga acgacaccgt actggacggt cgcgatgtca ccaaaaccaa
    10261 taccaccgac gtagcgacct tcaagtctgt taactacggt cctctgggat acattcacaa
    10321 cggtaagatt gactaccaac gtaccccggc acgtaagcac accagcgata cgccgttcga
    10381 tatctctaag ctgaatgagc tgccgaaagt cggcatcgtt tataactacg ctaacgcatc
    10441 cgatcttccg gctaaagcac tggtagatgc gggctatgat ggcattgtta gcgctggtgt
    10501 gggtaacggc aacctgtata aaaccgtatt tgacactctg gcgaccgccg cgaaaaacgg
    10561 cactgcagtc gtgcgttctt cccgcgtacc gacgggcgct accactcagg atgccgaagt
    10621 ggatgatgcg aaatatggct tcgtggcctc tggcacgctg aacccgcaaa aagcgcgcgt
    10681 tctgctgcaa ctggctctga cgcaaaccaa agatccgcag cagatccagc agatcttcaa
    10741 tcagtactaa tcacctcgcc ccggtaacgt gccggggctt tttcacttca gacttacgtc
    10801 cattgccaat tttcattacc ctgatgataa tcactggaat aaattattcc gcgcgagggt
    10861 tttcgggtga aaaagcaatg gattgttggc acggcgctgc ttatgttgat gactggtaat
    10921 gtctgggcag atggcgaacc gccaactgaa aatatcttaa aagatcaatt caaaaagcag
    10981 tatcacggca ttctcaagct tgatgtcatc accttaaaaa atcttgatgc taagggtaat
    11041 caggccacct ggtcagcgga aggcgatgtc tcttccagtg acgatctcta tacctgggtc
    11101 ggtcagttgg cagattacga actgctcgaa cagacctgga cgaaagataa accgataaaa
    11161 ttctcggcga tgttaaccag taaaggaacg cccgcgtctg gctggtcggt gaacttttac
    11221 tcttttcagg cggcagccag cgatcgtgga cgggtggtcg acgatatcaa aacgaataat
    11281 aaatatctga tcgtgaatag cgaagatttc aattatcgct ttagtcagct tgaatcagcg
    11341 ttgaataacc agaagaactc gattcctgcg ctggaaaagg aagtgaaagc gcttgataag
    11401 caaatggtgg cggcgcagaa agcagcggat gcatattggg gaaaagatgc aaacggtaag
    11461 caaatgaccc gcgaagacgc ctttaaaaaa attcaccaac agcgtgatga gtttaataaa
    11521 cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag aggtttatca accggcgatt
    11581 gcggcatgtc ataaacagag tgcagattgt tatgaagtgc cgattcagca gaagcgagat
    11641 ttcgatatca acgaacaacg gcgacagact tttctgcaat cacaaaaact tagccgtaaa
    11701 ttgcaggatg actggataac gctcgaaaaa gggcaatatc cgttaacgat gaaagtttca
    11761 gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg atgatatcaa ccagactaat
    11821 gaacgctgga aaaaagatac cgaacaattg cggcgcaatg gagtgattaa gtaactcatg
    11881 ttaagtacct ttgccggata atataaaaag ttatccggca aattattttt tacattagcc
    11941 ctagtagctg tggaatgaac aaggatattt gcgggatgta agtgatcatc atcaacgcga
    12001 ttaacaatgc ggcataaaat ggcagcaatg gtttgatcaa atgttgaatc ttcacacctg
    12061 aaatagaaca accaacaaat agcgcactac ccaccggcgg tgttaacagc ccaatacaaa
    12121 ggttagccac catcataata ccgaaatgta ccggatccat tcccagctct tgtgcaatcg
    12181 gcaagaagat tggtgtgaag attagtacag ccggtgtcat gtccataaag atgccgacaa
    12241 ttaataagac gatattaatc agcagcaata tgaccagtgg gttatcagaa atccccatca
    12301 acgcgtcgct gatcatataa ggaatatcag cattggtcat tgcccaggac atgcccacgg
    12361 aaaaaccgat caacagtaat acaatcgaag tggtaacaac cgattccaga atcagtttgg
    12421 gtaaatcgcg ccattttact tcccggtaaa tcagcaccga gagaataaat gtgtagacaa
    12481 cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc cagaatgccg cccattacaa
    12541 tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc tttacatgcc tgagcaaagg
    12601 ttgctctttc tgaaagtggg tacccacggc gtttagcaat aatgccgcag actatcatta
    12661 ccgcgagacc cattaagatc cctggcaaat atcccgccat aaatagcgat gctacagaaa
    12721 cgccgccagc tgtcagggaa aacacaatta acacgtttga gggcggtatc agtaatccag
    12781 taatacaaga tgagacgttt acagcggtag aaaatgccgg atcataacct tctttggtct
    12841 gaatgggatt cagtgtgcca ccaacggcag cagctgccgc aacggctgaa ccagaaatgg
    12901 aaccaaacat catattcgcc aggacgttca catgaccaag cgaaccagga acgcggccaa
    12961 ccattacctg ggcgaggtta atcaaacgta tcgcaatacc gccgctgttc atcagcgttc
    13021 cggcaaagat gaaaaagggg attgccaata gggcaaagtt atccagtcca ttagccagtc
    13081 gctgcgaaac agtgattgcc gcgatatccc agggaaacat caacagcatt gaagctacag
    13141 tggcaatacc gattgagaac gaaatgggca caccaataaa gaccagcaca aaaaaacagc
    13201 caaacagtgt aagcgcgata taactatcca ttaactgacc tcctgcgcag gttgtttcag
    13261 ttgttgaatg ctttgattaa taaaacacaa ggcataaaac atcatcacca atccacttaa
    13321 tggcagaatg atatatacgt atcccatcgg tatttgcatc gcggcagata cctgtgatgt
    13381 tgccagtgtt ttatcaatta atttcaggcc accagtcaca atgaccgaac cggcaaaacc
    13441 taaaataaga acgttaatga ctatactaag aagtaattgc ttgcgcttat tcagggcgag
    13501 agcaaataaa tcaatagaga gatgtctctg cgcgccgacg gtatatgcag cacctaataa
    13561 tcctacccag atcatcaaaa agcgtgccag ctcatcggtt aatgtacttg gctggttaag
    13621 tacgtagcgg ctaaatactt gccagactac gcatacaacc agagcaagca ttacggcgac
    13681 agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg cggttaaata tcattgtaag
    13741 catcctggaa aatcagggcc agagactggc ccgaaatatt attgagcagc gttgtcgaat
    13801 ttctctaaca aagcagcttg ttttggatct tttttgaagt cgtcaaacag aggctgaact
    13861 gcggcacgga atggggcttt atcaacctta acgatttcac cacccatggc tttcgcctga
    13921 gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt gataagcttc ggatttcttc
    13981 gccgctgttt cgaggatttg ttgctgttct ggggttaatt tattccatgt tttggtggag
    14041 ataaccaaaa agtcaggaat tgaggcatgt tcatcttcag aaaagacttt ggcaatttcg
    14101 atatgtcgag tctgcaccca ggaaggcacg ttattttctg cgccatctac tacaccttgc
    14161 tgcatcgcag tataaacttc gccaaatgaa attggagtgg gtgagccacc catcagttca
    14221 atcattttga tggtggtcgg gcttggctga acacgaattt tcagcccttt aagatcttct
    14281 ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac ccgctacata agctgataaa
    14341 gcaaaaaagc ctttatcttt tgttgaatcc ataatttctt taccaacttc accaaaaacc
    14401 actttgttga agtgcgcctg atctttaaat aagaacggca aattatagat agcataaata
    14461 ttatcaaaag actccagatc actggcggaa cctttggtca tatccagtgc accattttgc
    14521 aacaactaca tagtttctcg cgcattaccc atctggctgc tggggtaaat acggatcacc
    14581 atattacctt ttgacagctg ttttacttct ttcgccagct cttcaaaact ctgatgtaca
    14641 acatgacttc tttccaggtt gtgtgctaac tttaacgtca ctttttctgc ggcgtttacg
    14701 ccagtgctaa ccagagccag caaggatgcg cagactaatg agcgggtaaa tgacctcatt
    14761 ttcataaaaa aactccctgt tgaagaatgg aaattggttg atatctaatt gtgcttatca
    14821 gtgctttgtg cgacaaattg gttaacctgt ctgacaactt gtctatgttt atcacaactt
    14881 gaaataaagt cagaaattaa atattaattt tgtgcgccag gttgtacatg tttatactca
    14941 ataaatgatg agtaaatatt
//
LOCUS       DAZGTJ010000109        14825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000109 DAZGTJ010000000
VERSION     DAZGTJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.109"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..626)
                     /locus_tag="ACHK8E_004275"
     CDS             complement(<1..626)
                     /locus_tag="ACHK8E_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346465.1"
                     /translation="MNFEELINDIERLLIGKELQSINPNTPPLFLLKIDRDIGKYYVS
                     VSLNAKPTARSISEFEYIFNDLLRKGFCNVDQSLYGSSSSRNHPETIFANLPYIQFFK
                     FKKKKHILLRNKNVHEAGQLSELQGAESRAIRKQIENYLSLNLSEIAEQQLKSLEKMF
                     DAYDAILKKYPGDIPVIMAEQGLLGLKNISEVLINSYVSLDFDSSGDK"
     gene            842..1732
                     /locus_tag="ACHK8E_004276"
     CDS             842..1732
                     /locus_tag="ACHK8E_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346466.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1871..2065
                     /locus_tag="ACHK8E_004277"
     CDS             1871..2065
                     /locus_tag="ACHK8E_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0346467.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            2082..3365
                     /locus_tag="ACHK8E_004278"
     CDS             2082..3365
                     /locus_tag="ACHK8E_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP0346468.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            3349..3906
                     /locus_tag="ACHK8E_004279"
     CDS             3349..3906
                     /locus_tag="ACHK8E_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP0346469.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3896..4768
                     /locus_tag="ACHK8E_004280"
     CDS             3896..4768
                     /locus_tag="ACHK8E_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP0346470.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4928..5539
                     /locus_tag="ACHK8E_004281"
     CDS             4928..5539
                     /locus_tag="ACHK8E_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP0346471.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            5529..5954
                     /locus_tag="ACHK8E_004282"
     CDS             5529..5954
                     /locus_tag="ACHK8E_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFP0346472.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            6146..7951
                     /locus_tag="ACHK8E_004283"
                     /pseudo
     CDS             6146..7951
                     /locus_tag="ACHK8E_004283"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            8059..8241
                     /locus_tag="ACHK8E_004284"
     CDS             8059..8241
                     /locus_tag="ACHK8E_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346473.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            8525..8968
                     /locus_tag="ACHK8E_004285"
     CDS             8525..8968
                     /locus_tag="ACHK8E_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP0346474.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            9042..9422
                     /locus_tag="ACHK8E_004286"
     CDS             9042..9422
                     /locus_tag="ACHK8E_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346475.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            9458..10192
                     /locus_tag="ACHK8E_004287"
     CDS             9458..10192
                     /locus_tag="ACHK8E_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFP0346476.1"
                     /translation="MTQQTVVGSPQPSHPARSCKSTVEDAMKRLLTALLLFIFNGVAA
                     SPATPEVVRYDRYLLVSTSPQQPPLEQLTTLSVPPGFHPTVGEALQYLLRDSGWSLCL
                     SDARRAQLYGLPLPLNQYHTGPLRLKAALQLLAGPAWRMTADENRREVCFTSDHSLPQ
                     TVAYRHPVSLHPLGPLTAPFFLAGTVQRGDIRLAVIVPPEALYLHQVVWLRIGEQWHG
                     WRLARFDERRAVFRHGKRQLTLKVPE"
     gene            10196..10870
                     /locus_tag="ACHK8E_004288"
     CDS             10196..10870
                     /locus_tag="ACHK8E_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP0346477.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10849..11367
                     /locus_tag="ACHK8E_004289"
     CDS             10849..11367
                     /locus_tag="ACHK8E_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0346478.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            11364..13382
                     /gene="traD"
                     /locus_tag="ACHK8E_004290"
     CDS             11364..13382
                     /gene="traD"
                     /locus_tag="ACHK8E_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0346479.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            13379..14026
                     /locus_tag="ACHK8E_004291"
     CDS             13379..14026
                     /locus_tag="ACHK8E_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFP0346480.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            14092..14337
                     /locus_tag="ACHK8E_004292"
     CDS             14092..14337
                     /locus_tag="ACHK8E_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP0346481.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            14327..14611
                     /locus_tag="ACHK8E_004293"
     CDS             14327..14611
                     /locus_tag="ACHK8E_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0346482.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
BASE COUNT         3298 a         4486 c         4010 g         3031 t
ORIGIN      
        1 attttatcgc cactactgtc aaaatccaag gaaacataag aattaatcag tacttcgcta
       61 atgtttttta atcccaacaa gccttgctca gccattataa cagggatatc ccctgggtat
      121 ttttttaaaa tagcatcata cgcatcgaac attttttcca acgattttaa ctgctgctct
      181 gcaatttcac ttaaatttag agataaataa ttttctattt gcttccttat agcacgagac
      241 tcagcccctt gtaattcaga aagttgacct gcttcgtgta catttttatt acgtaataat
      301 atatgtttct ttttcttgaa cttaaagaat tgaatataag gcaagtttgc aaatattgtt
      361 tccgggtgat tacggcttga agagctacca tataatgact gatctacatt acaaaaccct
      421 ttacgcagta agtcattaaa tatatattca aattcagaaa ttgaccttgc cgtaggctta
      481 gcatttaaag aaacagacac gtaatattta ccaatatccc tgtctatttt taaaaggaat
      541 aatggggggg tgttaggatt tatagactgc aactctttac caattaagag tctttcaata
      601 tcatttataa gctcttcaaa attcataaca gaagccttta tccattgata ttatactaat
      661 ggatatcata ccatatttat gcctattcgt aatcattgat ttcctaaata aaacagtaaa
      721 aaacctaaaa aaaacaccaa tagatcacat acaaaacaac cagttaaaag aactcacctt
      781 acctcgcttt agttggcttt tttagatagt actcatcata tgtaactaat aggagttacc
      841 catgccccca cacaaaattc gccaagagtg gctgacgctg gatgaggctg cagaagtcat
      901 ccggcagcac aacatcacgt tgctgcccgc cgacctgatc cgccatgccc tgcacggcga
      961 tctgtcgctc tcggtctact tcccttcgcc cgttctgctg agaccactca atccgccttg
     1021 ccgctttgcc tgccgattca gtaagcctcc ttttccacct tcgcagtgca acaaacccgg
     1081 caaccgcaaa gtgatggcaa actcgccgcc gtgcagacgc gacccaactc tgcaactgac
     1141 caccagccat ctgtggaatt tgccgttaat cggcattgag cgcctgctgc tccaccagca
     1201 actggcgaaa acgttgaatc gcccttgccc caatgatacc cattgctgca accagattgg
     1261 catcgtggtc tgcgatgaaa acgacagcta ctaccaactc tgttgccgca aatcgttgcg
     1321 ggagctttta ctggaactgc tcgccacgca aaacaacctc caggaagagc ttcatctgct
     1381 gattggccag ctatatggta atggccacaa tgacctgaaa tcccggctca gtctgtccgt
     1441 ctgctacctg caggacacgc tgcctcctga cgcactgttc atcatccgcc gcactcatct
     1501 cgaagccttc ctgacccgtc atcagccaga atcaaaggag cggatcacct ccgcgctttc
     1561 gcgtttgctg tggctggcct gcaagcacaa cccgcaactg gacgatgaaa tgctggagca
     1621 tccctacaaa ctgctgaata ttttcgaatg ctgggcgcgt gaggaaggta tgcacctgcc
     1681 gctgagcccc gagacgttga aaaccgcact ccagcgcggc gcaccgccgt aacgcgatca
     1741 actaaccctt tcaactaaca actctcacta acccagataa ggatttttac tgcctggcag
     1801 ggacgctgct gctttgataa ccacgcctat cgtgctgatg tgaacgaatc tggacacagg
     1861 aggaatggtt atgaatagcg acagattttt acgcttacgc caggtggaag ataaaatcgg
     1921 cttcggtaaa tcgtggattt atcgacaaat ccagctgcaa cagtttccgc cgtccattcg
     1981 actcaatagc cgccacgtcg cctggctgga aagcgaggtg gacgcgtgga tccaccagcg
     2041 catccggctt acccgcgatg tctgaatgcc ggaggaagat tatgtgcgct tcacacctgc
     2101 tcaaccgctg gcttcacggc gccagcaata ccggagaaca ggaaatggtc caggtcagcc
     2161 tggagcaaat ccagccgttc tccctcaatc cccgcgtcac ccgcaacccc ggctatgacg
     2221 tgctgaaggc gtcaattctt gcccgtgggc tggataaccc gccggtactg acccgccgcc
     2281 ccggtgatga aaaatatatg ctcgccagcg gcggaaatac gcgcctcgcc atcctcaatg
     2341 aactgtggca ggagacgcag gatgagcgtt accaccgcgt ctgctggccg ttcagaccgt
     2401 ggccggaaac gctgtcaccg cagcaaggcg aagtccagtg cctgatcggc catctggccg
     2461 aaagcgacct gcataacgga ttgatgttta tcgaacgggc ggaaggggtc ctgcacctgc
     2521 gcgatcttta cctcgaggca ggcattgaat gcccgacgca gctggcgctg gcggaacaac
     2581 tcacccgcga cggctatccc gtcagccagt cgcaaatcag ccggatgcta cagaccgtag
     2641 actggctgct gccctgcatc ccaaacgcgc tatatggcgg tcttacccgc aaggttatcg
     2701 atcgcctgct gtcactacgc agcgcagcag aacaggtctg ggtaaagctg atgccgctgg
     2761 aacgccagcc cgaatttgcc gacctgttca gcaccgccct cgccatcttc aacggcgagc
     2821 cggaagccgt ggtaccgcaa ctggtgcagg acgaactgct cggtgagatg agctgtcgca
     2881 gcggcatgag ctggaatacg ttgctggcgg aactgactga cggtcagagc aagcggcagg
     2941 tgctgctggg gcctccggcc gaaaaggtcc tctggccgcc accgccggaa gtggaaaaag
     3001 caccactacc agagcctgag ccaccgactg aacccgccgc gcccgatgag ccaaagccct
     3061 caccgctggt gcatacggca cagtcgctgt gtgcctgctg gggactggac gcctgtatcc
     3121 agccggacga cagcgctccg gccggtttca ccctcgcgcc ttgccacgat tttccccacc
     3181 cggcggcaca gcgctgctat caggtgctgg cggccctgaa tggcgacacc agtacgccga
     3241 tgcccgccct cgaattgttg ctgtcgccgg actacaacaa cgctcagatc cagcaactga
     3301 tgcagcttat tagcctgtgc cgccagcggc ggctgcggga ggtgcgccat gacaccctca
     3361 gttaaccagg cagtactcac ccacatcgtc caggcgctga aggaggggca gatccgctac
     3421 tgcgaatcgc tcggcttcag cccgcaggag ctgtgcgagc tgacgcgcct taccgccgac
     3481 gacatcctgt ttctcagcaa cagcgcggtg cagttcatca ccacccacat tgatcacgag
     3541 atgctgggcc ggatgctcgc gaggatggag caggagcggc tgttccagca gcgtctggag
     3601 gaagcgctgt cgctcggcgc gtccatcgag ttcattaacc actattttgg cctttccacg
     3661 ccggaggtct gtgcccggcg gcggctgatt ggcctgtttg tccgccaggg gcgcaacaat
     3721 gccccggatg agcaggtgga gacgctggtg tggaacgagt ggcagaagac gggcgtgaaa
     3781 aagctcgact cacccgaagc gctaaccgcc atgatggcaa tggcccgcga acacgatctg
     3841 tcgctgacgg tcatctggaa cctgctgcgc cagtggactc aggagaagct gctgcatgaa
     3901 gagtgacgat cggctgctgt ttctcggcaa cccgcagcag agcgtgccga agcgcctgct
     3961 gctggcgcgc aacctctccc cgcgcgacaa gttcgcgtgg caactgctgc gcctgcatgc
     4021 ccgcgacgac ggcagcggcg tcttcccctc ctacaacgaa ctgcagggct ggctctctga
     4081 ccagcctgaa ggcgagaagg cctcgcgcag taccgtcagc aatgcactga tgatgctgcg
     4141 gatcacccgc tggctgagcc tgtgccagcg gctgcgcgac gagcaaagcg gcaggatcac
     4201 cggcaacctc tacgccctgc atgatgcgcc actgactcat gccgatgcct gtcggatgga
     4261 cagtgaatac ctgtcgctgc tcaccgcttg ttcccgccac cgcaaccgac gcatccgaaa
     4321 catcgctatc gggctgctgc tcaccctgca acaacctgtg gacggtggac gaacctgcac
     4381 tgagtctcca ttgaagaccg cccctggtct taatcgagga ctcagtaaaa aatgcccgag
     4441 tcttcaatta agactcagta atgtggcggg ggctggcggg cagccggagc cgcattgcgt
     4501 acatactggt acagatttaa acaataaaca tcaaaagcgt acgccagacc tgcactggcc
     4561 tgtggataat cccttcagcg ccagcgagcg ccgcgccgca gaacgcgcga tgcgcaatct
     4621 ggatcccgct ctctgccagc aagtgctgga cgactgcctg tggcggatcg ccgcccacga
     4681 tatccaccgc cccctcgcct atctgctggc aacgctggag aaagccagac ggggcgagtt
     4741 caatcagctt cggcgcaggc gcagatagcc ctgattttat gcgcaccgca taaagccggt
     4801 gtaccaacta tcaatcaaac ctcattcatc ggcgaaatta actttctgcg gaccagatgg
     4861 gtttcccgtc gcctgactca ttgtgcggac cgacactgcg ggcatctgaa tccatggaga
     4921 atttccgatg cctggcacaa ccctgttcag cgatatccgc atcaccctgc atacccgcgt
     4981 cgccgcccgg ctgtggcagg cgcatcccac tggcatgctg ctctgtctgg cgttactcag
     5041 gcgtctgctg cgcgccgaag aggccgacga tccgtgggcc gcccactggc tgaagcaact
     5101 gcgcatccgc ctgaacctga tcgcccacct actgaagcaa aaaaaccgtc gtctggacca
     5161 tgctttcgcc agcctgccag gcgctatcca caccacgcag gtcagtaacc cgtccccgac
     5221 cgaattcatc ctgccgctgg cgctattctc accgcccggt tcgcgcctgc tgcaacagtt
     5281 gattgattac gatctgctgg tccgccgcac cctgcttgcc tggcatctcg gccttatcac
     5341 ccaggcggag aagcgcgatt tcattgccac catcccacgc ctgatgctcc aggtgttcag
     5401 cttcgtgaac cgctttcgca ccactggcgt gactcgcgcc gacgtgcggg cgaacagtcc
     5461 gctggcgcag tcgatggcgc ataagctcgg aaatttgccc aaaaagatgc ttacggagat
     5521 acagcgcgat gttcaatgac gatgatgatg aacgctccgc ccgcaaaagc tcgctaaagg
     5581 gccttttaaa ggcctttaag ggtaagggag agctgcgcgc gctggtacag gaattacagg
     5641 cgctgcgctt gcagctgcaa caaaatcagc cagtgctgaa acaggcggtg tatgaactgc
     5701 cggggcgtaa tgtaccgctg attctgtgca gcaacccggc gcgctccggc gtcagctatc
     5761 tgcgctggcg caatctggac aacaaccgtt cggggagcgt ggcgttgcaa gaggccattc
     5821 aacagccgga cgcgagcgat gaactgcgcg acgcgctgat ggcgctcgaa tggcggcggc
     5881 atgtgctgaa tacgcagctt tcagtggtga tgtttatgct gcggcggata caaccgttaa
     5941 ccaccctggt ttaaaatttt gcgcaaagtt aaaccctgag tgtcacttta tcgattattt
     6001 taaacttaac tcatgcacct taaaacaggc actgtgcgct taatgcacgt tttaaggtgc
     6061 aatttttacc ttctcctacc agtttcccct gtcgccttat ccctccggca caccatgctg
     6121 aactcccttt caccggagac agcccatgca actttacctg tgcgaaaaac cctctcaggc
     6181 acgcgacatc gcccgaattc ttgacgccca taaccgcagc gacggctacc tgcataacgc
     6241 caccgtaacc atcacctggt gtttcggcca tctgctgagc atggccgcgc cggaggatta
     6301 cgatccggcg ctgaaacact ggcggacgga acagctgccg tttattcccc cggcatggcg
     6361 gttggtgatc cgcccggagg tgcataagca gttccgtatc atcgaaaagc tgctgaaaca
     6421 ggcggatgca gtggtgattg ccaccgacgc cgatcgcgaa ggcgaaacca tcgcccgcga
     6481 aattctggaa caatgccgct ggcgcggtcc ggtccagcgg ctgtggctgt cggcgctgga
     6541 tgaggccagt atccacaagg cgctcgccac tctgcgtccc ggcgacagca cctttccgct
     6601 gtatcaggcc ggactggcgc gcgcccgcac cgactggctg gtcggcatca acatgacccg
     6661 cgcctgcacg ctaatgaacc ggcggcacaa gggcgtgctc tccgtgggcc gggtgcagac
     6721 gccgaccctg cgtctggtgg tggagcgcga ccgggagatc gcccgcttca cgccccgccc
     6781 ctggtggcgg gtggatgccc gtttgcatgc ggagggttgt tcctttctgg cgcagtggca
     6841 gccggatagt gcgtactgtg acgacgaaag ccgctgcatc cgcgaatccg ccgcccgtga
     6901 ggccacagca cgcctgcaac aagccggaac cgcgctggta atggacgtga caacccggcg
     6961 ggagaaaacg ccgccaccgc aggccttcac tctcagcgcc ctgcagcagg cctgctcaaa
     7021 acagtggggc atgggcgcac aggaggttct ggatatcgcc cagctcctgt atgaaaccca
     7081 taaagccacc acctacccgc gcaccgactg cggctatctg ccgctctcca tgcacgatga
     7141 agcaccacaa gttctggcgg cgctggtcgc cagcgacgct tcactccagc ccctcgccac
     7201 gcagcttaat ctgcagcaac gctcccggtt gtgggacgac agcaaaatca ccgcccacca
     7261 tggcatcatc ccgacccgta aaacggtaga tctgacgcag atgaacgaca gcgagcgcaa
     7321 ggtctacggc ctggtacgca gccattttct ggcgcagttt ctgccagacc acgaagtgga
     7381 taaaacggac ctgcgcctga actgcgccgg ggaaacgctg actgcccgtg gctcagtggt
     7441 gattgtcagc ggctggcgac aactgttttt gcgcgaaatg gcaacgcact caccggaaga
     7501 aacgcaggca cttcccgccc tgcaacaggg acaaacctgc taggtggaac aggtggacgt
     7561 tcgcgcccgc cagacccagc caccggagca ctacaccgaa gggacgctga ttgccgcgat
     7621 gaaaaatgcc gcccgttttg tcagcgatga acgcctgaaa cagcggctga aagaaaacgc
     7681 gggcatcggc accgaagcca cccgcgccgg gattatccag acgctcctta agcgcggcta
     7741 tctgattaag cagcgtcgtt tcctgctggc aacggatacc gccagcacgc tgattgacgc
     7801 cctgccggaa acgctgaaag atccaggcac cactgcactg tgggagcaaa tgctggatga
     7861 catcgcgacg gggaaaatgg gactggaggc gttcctggtg cagcaacagc agaacgtgac
     7921 agagctgatt aagtcgttac ggaatgagta aataacctgt ttcctgcatc acacttttac
     7981 ttttccctga ttgctcgttc aaattggcag tctgatgatc ctctctgagg aatattcctc
     8041 atttttcgga gtgttaagat ggccaagaat accggagacg gttatcgcaa gggctctgtt
     8101 acagaccgca cacaggttca gaatccgcag aatggcaact acaccaaacg cgatacaacg
     8161 acaggtcgct ttatcgacca gaagcaggac ggcactccct ttaaaggtgt cgccaaagaa
     8221 gtcgatgacc gccggaaata atatttagct aactttcagg gccttagtta ttaaggcctt
     8281 tttgcattct gtacttccaa ctttcacgcc gtaaaaacaa ttccaccttt cgtttctccc
     8341 taccgaagat tcaactaaac aggtcaaccc caaaacccgc catgcattcc tgaaacgcgg
     8401 aggcgcacag cgcggaatgc ccgtactgcc agtaactcag aggccttgca gccaatgagc
     8461 caaacccgaa gcggggcgtt cccgcgcatg gggcgctccg caccttcaac acggagatac
     8521 caccatgaac gatacccact acttcaacct gtacaccagc ggtctgggct acctcaaccg
     8581 gatccgggaa gtgaccccga aaaaaggcga accgttcctg tgttgcgaca ttgccgcact
     8641 acggggcgaa gccagcaacg cggagcacac ctggttcgac tgccgcgtca gcggcaagga
     8701 agcgcagaaa ctggtgcgcc gctgtatcga cgcctgcgag cagaaacgca aggtgctgat
     8761 ccagttccgc ctcggcgatc tgtacgccga cacctttgtc tacagcaaag gcgaccgtaa
     8821 aggcgaaacc ggcgtcagcc tgaaggccca tctgctgcac atcgggctca tcaaaattga
     8881 cggtgaagag gtttaccggg cagagcgcga agctgaacct cagccagcgc cagccgcact
     8941 gccagcagag cctgtagcgc agttctgacg cacagcattc accctgccgg ggaaattccc
     9001 cgtcggggcg tttccctggc cttttaccgg agagacgcac gatgaatacc gtattgaact
     9061 cacgcgaaga agcccttgac cggggcaaac tgattaacct tgatgacgag gcgctggacg
     9121 ctggcttgcc actggatctg gccattaccc ttccggccca caagcactgt cttgaagacc
     9181 ggaatgacaa cgccatcagg gacttgctga aaggtgttga aatggcagcg gtagtggcct
     9241 ttatctccca ccaaccgccg cagcgcacca ttcgtatcga ttaccgctcg gtgctgacgc
     9301 aaaaaaccaa cggcagcacg cagcagcaaa ccgtcagatt gacgttacgc gttcattttt
     9361 ctgagcaagg catccccatc gccgccacgc tggggctgat gggggaaaga ttcccttact
     9421 gatctgattt tgcgcccgga ataacgggcg ctattttttg acgcagcaaa cagttgtcgg
     9481 tagtccccag ccctctcatc cggcacgctc ctgtaaaagc actgtggagg atgcgatgaa
     9541 acgactgctg acagctctgc tcctgtttat tttcaacggc gtggccgcct cccctgcaac
     9601 gccagaagtc gtccgctacg accgctatct gctggtcagc acgtcaccgc aacaaccgcc
     9661 actcgaacag cttaccaccc tgtccgttcc gcccggtttt cacccgacag tcggtgaagc
     9721 attgcagtac ctgctgcgcg acagcggctg gtctttgtgt ctctccgatg cgcgccgggc
     9781 gcagctttat ggcctcccgt taccgttgaa ccagtaccac accggcccgc tgcgcctgaa
     9841 ggccgccctg caactgctgg caggcccggc atggcgaatg accgcagacg aaaaccgccg
     9901 tgaagtctgt tttaccagcg accattccct cccccagacg gtggcttacc gtcatcccgt
     9961 gtccctccac cctttggggc cgttaacggc cccgtttttt ctcgccggaa ccgtacagcg
    10021 cggcgatatc cgactggcag tgatcgtgcc gccggaggcc ctttatctcc atcaggtagt
    10081 gtggctgcgt atcggggagc agtggcacgg ctggcggctg gcgcggtttg atgagcgccg
    10141 ggcggtattc cgccacggta aacgccagct gacgctgaaa gtaccggagt aagcgatgaa
    10201 acgcctgatt ctcctgttac tgttcaccgc cagcacgtgg gcgcaaccgc ttcaggtgca
    10261 gaccagccag aacgtgggtt cagccctgca accactgacc acgcagcagc aggccaccgt
    10321 ctggggcatc acgccagagg agtggcagcg ctatctcacg ctgaaacagc aggcgcgcgg
    10381 cgtctggtcg ccggggctcg atccactgac cacactcggc gtagaagccg acactgacca
    10441 cgaacgccag cgctttgccg aactgctggt caggcaggag taccagcggc tggagaaaga
    10501 actcgccttc cagcgtgctt atgatgccgc ctggcagcgc ctgtatccgg gcctgacgcc
    10561 gctgaaaacc acctctgcca gtacttcccg cgtttcgcta tttgttaaag atgactgccc
    10621 gccctgcgac agcctgctgc gtaccctgct ggcgcaacag cgcccgctgg atatctggct
    10681 ggttgggagt gaaggcgatg ataaccgggt gcgccgatgg gcgatggcgc atggcatcga
    10741 cgcaaaactg gtgggtgaac acagcatcac gcttaaccac gacgcgggcc gctggctcct
    10801 gttggggcag agcaaaatcc cggtggcgct ggaacgtaag ggggagcaat ggcgcgaggt
    10861 cctgccctga tcctgctgct accctgtctg gcgctggcac agccgctgcc agaagcgtac
    10921 ctgcgcaccg cggagcattt ccgcgtgccg ccggagctgc tgtgggccgt cacacaaacc
    10981 gaaagcggca cgaacctgag cggcagacat cttccgtggc cgtggacgct caatatcagc
    11041 gggaccggct accgctatgc cagccgggag caggcctgcc aggcgctgct ggttgccatc
    11101 cgccgcacca gcccaaagcg cattgacgtg ggactcgggc aaatcaacat cggctggcac
    11161 caggcatatt ttgactctcc ctgcgacgcc ctgtatcccc ttcaaaacct gtcgctcacc
    11221 gcgcaactgc tgcgtcagcg ctacgatgaa cgtcccggaa gctggctcaa tgccgccgcc
    11281 cgctatcatc gtcccgctgg cggcgcgcct gccgcgcgat accgcaagct ggtcagccgc
    11341 catctgcaac aggcctcacc gccatgagcc gctacgcgct ggaggcgctg ctgcgcccac
    11401 ccgtggagtt ttacagcacc gtcatcgctg gctgtggcgc attaatctgc ctgacgcagg
    11461 ccccgctgct gatgctgacg ccgtggcagg cccgcatgat ggccagtgcc ctgatgcttc
    11521 tggccctgcg gcgcggctgg cagggatggc aaatcgtacg ctaccagcgt aacctcctgc
    11581 gcctgccgcg ctacgcgctc accagccgga aaatccccgt cagccgccag aggctgtttc
    11641 tcggtaaagg ctttcgctgg cagcccatcc acacacagcg tctgtacgac tgcctgcaac
    11701 cggaagccgc acgctggctg cgccccggat ggtgttaccg cctggcaaga cacatcgaaa
    11761 aacgcagcga gcataccctg cccggccttg cccgactgct cagcgccgat cgctggctga
    11821 acccagtacg tccgctgcca cccgtaggcg gcaatccgtg gttgcatggg gtggaactaa
    11881 aagagtgcga cgtcactctg ccgctcagtg aacgccccgg ccatacgctg gtgctcggca
    11941 ccacccgcgt tggcaaaacc cggctggcgg aactgcttgc cactcaggac atccgccgtg
    12001 gcgaggtggt gatcgttttt gatccgaaag gcgacgcgga tctgctgaag cgtatgtgga
    12061 cagaagcgca gcgcgccgga cgcggcgacc gctttatcct gttccatctg gcgtacccgg
    12121 agttctccgc ccgctacaac gctatcgggc gatttggccg catttcggag gtggcgtctc
    12181 gcctcactgg tcagctttcc ggcgaaggca actcggcggc ctttcgcgag ttcgcctggc
    12241 gctttgtcaa catcatcgcc cgcgccctgg tggcgctcgg ccagcgcccc gactatcagt
    12301 taatttcccg ccacgtcgtc aatatcgacg ccctgtttat cgactatgcc cgcatcgtgc
    12361 tgccgcgcca caacacgcgg tcatgggaag tggtggtgca actggcggag cgcgttaacg
    12421 acaaaaacac gccgcgccac atgctgggcc gcgatcctca cgtggtggcg ctggagcagt
    12481 acctcaacgc ggaaaaatta tacgatccga tcctcgatgg cctgctctcg gcggtgcgct
    12541 atgacaaaac ctatttcgac aagattgtcg cctcactgct gccactgctg gaaaagctga
    12601 ccaccggcac gcttgccgcc ctgctggccc cggattacca cgatctggac gacgcgcgtc
    12661 cgatcctcga ctggcaacag gcgatccgcc agaaggcggt agtctatgtc gggttggacg
    12721 cgctctccga ttcggtggtc gctgccgccg tcggcaattc gatgtttgcc gatctggtga
    12781 gcgtcgccgg gcatctgtat aaatttggcc tcaatgatgg actgcccggc aatagcgaaa
    12841 aaccgccgcc tatcaatctg cactgcgatg agtttaatga actgatgggc gaggagttta
    12901 ttcccctggt caacaagggc ggcggtgcgg gcattcaggt caccgcctac acgcagacgt
    12961 tgagtgatat tcaggcccgt atcggcagcg caccaaagac aggccaggtg gtgggtaact
    13021 tcaacaacct gattatgctg cgggtacgcg aaaccgccac cgccgaactg ctgacgaagc
    13081 aactgccaca ggtggaagtg cgcagccgcc aggtcagttc aggtgtcacc gacacgccgg
    13141 gcggcatcaa cggctttacc agcaacacct gcgagcaggt gaccacccgc aatgtaccgc
    13201 tgctggatac cgccgcgctg atccagctcc ccaaaggcca ggcttttgcc ctgctggaag
    13261 gcgggcagtt gtggaaaatt cgtctgccgc tgcccgtagc ggataagcaa tgcccactac
    13321 cagattctgt cgcgcaactg gcggcgtgga tgcaggcgcg ccagcagggg ctaacggaat
    13381 gagtgagctg cgaccgcctc cgtcacgccc accacaggga ccgattggct ggctattgtc
    13441 actggccgga cggattattg ggctggttat cggtacgctg gtgctgcgag tggtgctgga
    13501 actagtgggg ctatatttct ggtggccgca ggaagggagc aggcatgttt ttcaggtgat
    13561 gaggcaggag aaggtggaac tgattattgc ggtacagcca catcctttgg ggaaagagat
    13621 acgagtactg ctggagcatg gaataacccg cgttaatgat gtcagacata aggccgtgag
    13681 tcggttgttc cacgaacccg ttaccgtcct ggcttatacc atgatcagct ttgcgctgcg
    13741 cctgacgtgg ctggttatca tgttgccact gctgtgcctc agtgctgtaa ccgggctgac
    13801 agaaggcctg gtgcaccggg atttaagacg gtttggctcc gggctggaat ccatttttct
    13861 ccaccgttat gtcatcaggt gcggcagttc agtcaccacc acgctttggg tggtttatct
    13921 ggcgcagcca ttttctctgc ccgcgatgct ggttttactt cctgccgcct tatggtccgg
    13981 gatagcggta tggatggcag caggaagctt taaacgttgg ttataagttg ctcaaaaaat
    14041 cgcacctcac tacaatgtat acaatgtgta catgtgtatg aggccaccaa tatgggcgta
    14101 atgtcagtcc gtttaaacaa caatctttcc acgcaactgg aagcattatc aaaagcgacc
    14161 ggacgctcaa agtcctggct ggcaaatcag gcaattgaag attatgttgc ccgggaggcc
    14221 tggcaagttg cagagattga gcaggccatt caggaagctg atgccggtga ttttgccact
    14281 tcagatgagg tggataagct gtttcaacgt ctgggagtaa agccagatgg aaattaagtg
    14341 gctacgtaaa gccattgcca atctcgaagc tgagttccgg tatattgctg aagacgatcc
    14401 gcaggctgcg gaacggtttg tgaacgaagt ctggcgactt acgcaactgc tcaaagagca
    14461 acccgctatg ggccgggaag gaagggtacc tgaaacacgt gagctcattc ttcataatta
    14521 cccttatatc attccctatc gagtacgaaa taatataatt caggttttac gcgtgtttca
    14581 tacccatcgg cgcttacctg taaattggtg ataaaaaaca caagacaaga aattttttag
    14641 agatggaatt caaaccatgg acaaacctat ttttaatatc ataaacaaaa aattcattat
    14701 ttcagtagta acctcatcta ccttcatcac tggattcacg atagcagtta tatggtttta
    14761 cctatcaaac attgatagat tagatatttt tttcgatgct gtaaacctca gttcaacctt
    14821 aggga
//
LOCUS       DAZGTJ010000110        14391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000110 DAZGTJ010000000
VERSION     DAZGTJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.110"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1911
                     /locus_tag="ACHK8E_004294"
     CDS             <1..1911
                     /locus_tag="ACHK8E_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0346483.1"
                     /translation="RLTQKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVVVKPSD
                     GEGTVISATSDPEYYFVVVLAGQSNGMAYGEGLPLPETYDRPDPRIKQLARRSTVTPG
                     GAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANA
                     GILLVPCCRGASAFTTGADGTYSESAGASENSLRWGVGKPLYQDLVSRTKAALAKNPK
                     NRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELAGQASQSTGGSASAVPWVCG
                     DTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDENGANVQTNDPAEDPDLEAVG
                     YYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAILVHAGRTAEFITGKQPDTV
                     KPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWRYYHKPASGDSVDKNIAEAV
                     VSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELLKKGGRVSVRFKVPGALVPNQFA
                     LGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAMYHKKQNAKLDTFGVFDNGW
                     HTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKLQLTGISKAATYTLLIDS
                     VKVEVNNADAAA"
     gene            2062..2244
                     /locus_tag="ACHK8E_004295"
     CDS             2062..2244
                     /locus_tag="ACHK8E_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0346484.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            2282..2551
                     /locus_tag="ACHK8E_004296"
     CDS             2282..2551
                     /locus_tag="ACHK8E_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0346485.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEEPQPDGEMMM"
     gene            2627..2842
                     /locus_tag="ACHK8E_004297"
     CDS             2627..2842
                     /locus_tag="ACHK8E_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0346486.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2846..3073
                     /locus_tag="ACHK8E_004298"
                     /pseudo
     CDS             2846..3073
                     /locus_tag="ACHK8E_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            4149..4682
                     /gene="rrrQ"
                     /locus_tag="ACHK8E_004299"
     CDS             4149..4682
                     /gene="rrrQ"
                     /locus_tag="ACHK8E_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0346487.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            4901..5014
                     /locus_tag="ACHK8E_004300"
     CDS             4901..5014
                     /locus_tag="ACHK8E_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346488.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            5016..5783
                     /locus_tag="ACHK8E_004301"
     CDS             5016..5783
                     /locus_tag="ACHK8E_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP0346489.1"
                     /translation="MNNNSAKPFLPWLGGKRRLAKHILPLFPEHTLYVEPFCGGAALF
                     FMKKPSCAEIINDINSDIVNLYRVVKYHQEEFSRQFQWMLASREMWNEVINQHTEYMT
                     DIQRAVRFFFIQKCAYGGRAYSRAFGVKTHRPQIIPGNTEQLLQETRDRLARATIEHL
                     DWEVCIARYDRPTTLFYCDPPYWLDGGYGIDFSFCNYERMAELARTIKGKMIISLNDT
                     QEMRRLFEGMNIHHVAHRYTVPRGKTSSVSELIICNF"
     gene            6021..6206
                     /locus_tag="ACHK8E_004302"
     CDS             6021..6206
                     /locus_tag="ACHK8E_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0346490.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            6292..6516
                     /locus_tag="ACHK8E_004303"
     CDS             6292..6516
                     /locus_tag="ACHK8E_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346491.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            6861..7187
                     /locus_tag="ACHK8E_004304"
     CDS             6861..7187
                     /locus_tag="ACHK8E_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP0346492.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAVANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(7319..7519)
                     /locus_tag="ACHK8E_004305"
     CDS             complement(7319..7519)
                     /locus_tag="ACHK8E_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0346493.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            7561..7926
                     /locus_tag="ACHK8E_004306"
     CDS             7561..7926
                     /locus_tag="ACHK8E_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0346494.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            8215..8778
                     /locus_tag="ACHK8E_004307"
     CDS             8215..8778
                     /locus_tag="ACHK8E_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0346495.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            8775..10436
                     /locus_tag="ACHK8E_004308"
     CDS             8775..10436
                     /locus_tag="ACHK8E_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0346496.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            10500..12437
                     /locus_tag="ACHK8E_004309"
     CDS             10500..12437
                     /locus_tag="ACHK8E_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0346497.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AAPGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            12482..12703
                     /locus_tag="ACHK8E_004310"
     CDS             12482..12703
                     /locus_tag="ACHK8E_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346498.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            12649..14391
                     /locus_tag="ACHK8E_004311"
                     /pseudo
     CDS             12649..14391
                     /locus_tag="ACHK8E_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         3437 a         3370 c         4375 g         3209 t
ORIGIN      
        1 cgactgactc aaaaactgaa ggaggggtgg cagccatttg gcagccctgt cgccatcacg
       61 ccttataccc tgatgcaggc cattgcggcg gaaggtgatg tcaccacacc tgtggtggtg
      121 aagccgtcgg atggagaagg cacagttatc agcgccacca gcgacccgga gtattacttt
      181 gttgtggttc tggcagggca gtcaaacggc atggcatatg gtgaaggcct tccgctgccg
      241 gagacatatg accgtccgga cccgcgtatt aagcagctgg cgcgccgcag tacggtgaca
      301 ccgggaggtg ccgcctgtaa gtataacgac attattccgg cggaccactg tctgcatgat
      361 gtgcaggata tgagccgtct taaccatccg aaagcggacc tgtcaaaggg gcagtacgga
      421 accgtggggc aggggctgca tatcgccaaa aaactgctgc cgtttatacc ggcgaatgcg
      481 ggcattctgc tggttccgtg ctgccgtggt gcttcggcat tcacaacggg tgcagacggc
      541 acatacagcg aatcagccgg tgcatcggaa aattcactgc gctggggtgt gggtaagccg
      601 ctgtatcagg atttggtgag ccggactaaa gccgcactgg cgaagaaccc gaaaaaccgt
      661 ctgcttgcgg tggtgtggat gcagggagaa ggtgatgcgg cagtggggac gcatgcgcag
      721 catccggggc tgtttagtgc gatggtgaat cagttcagaa cggagcttgc cggtcaggca
      781 tcacagagca caggaggcag cgcatcagcg gtaccgtggg tttgcgggga cacaacgtat
      841 ttctggaagc agcgtcatgc ggaagggtat gcatcagtat acggagggta taagggtaag
      901 gagtcgcaga acattttctt tgtaccgtta atgacggatg agaatggtgc gaatgtgcag
      961 accaatgacc cggcagaaga cccggacctg gaagccgtcg gttattacgg ttcaaagtgg
     1021 cgtaatgacc agaagacctg gacatctgtg gacagggcca gccatttcag ttcatgggct
     1081 cgccgtggga ttatttccga ccgtctggca acggcgattc tggtgcatgc cgggagaacc
     1141 gctgaattca ttaccggaaa acagcctgat acggtgaagc ccaccggacc ttccggtgaa
     1201 ggcccggaga gagagccgga agccccggtc agtaaccgaa ccctgatgag tctgctggcg
     1261 tccggtgaag acctggcatc acagggctgg cgctattatc acaaaccggc gagcggagac
     1321 agtgttgata aaaacatcgc tgaggctgtg gtcagtgatg agggtgccac cggtggtaag
     1381 gccctgcaac tgaacaaacc ggaaagccac atctggtttc tggagcatga tgccgccggg
     1441 cagggggcag agttgctgaa gaaaggcgga cgtgtgagcg tgcggtttaa ggtgccgggc
     1501 gcactggtgc cgaatcagtt tgccctgggt atttactggc agttgtcgtc cctgccggag
     1561 ggagtgacgc tgtcagagga aggcaacgac atgctgatgt ccttcttcct gcagacggat
     1621 gcgacgaacc tgaacgcgat gtaccacaag aagcagaatg cgaagctgga tacgttcggg
     1681 gtctttgata acggatggca cacactggct tttgagtttg ccggaaacaa cagcattcag
     1741 gtgacgccgg tactggatga gaaacggggt acaccgttca cactggtgaa atctccggca
     1801 tcaggggcgg cggacaaact gcaactgaca ggcatatcaa aggcggcgac atatacgctg
     1861 ctgattgaca gtgtgaaggt ggaagtgaac aacgcggatg ccgcggcatg ataaaaaagg
     1921 ccgccagcaa tgaaggaaag ggatgaactt tgtccactac atgaatgctg gcggctggta
     1981 aaacgaccat gatgacaccg aagtctgcgg tgtttttcta caaaaataag taaatcatgt
     2041 caactatcac ggtgagaaac catgacattt ctgaaccagt taatgctgta cttctgtacg
     2101 gcggtctgtg tgctgtatct tctttcgggt gggtacaggg ctgtgcgcga tttctggcgc
     2161 aggcagattg ataaaagggc cgcagagaaa atcagcgcca gtcagtcagc cggaagcaaa
     2221 cccgaagagc cgctcattcc gtagtcactt tcttaacaac acctttcaac gagaaaatcc
     2281 tatgtcagaa ataaaatcgc tggtcactgc tgaagcagtg aaggaagtcc tgcgctctga
     2341 agaagtcaga agcgcactga aacagcaact tcggcagaac cttgaggcgc gtcttgatgc
     2401 agaagtggat gccattctgg atgaactgct gggcgcaccg actgttccgg agccggaagg
     2461 cattgcggat gacagtgctg tttcagatgg cgtcgggtct cagcctgacg gtagtgaaga
     2521 gcctcagcct gacggcgaaa tgatgatgta accatgcgca gggggtgtcg gtgtgagctg
     2581 atgccccact tgttgttgtg agcttccgga ttgcgggaga cggggtatgt accagatgga
     2641 aaaaatcaca acaggtgtgt catacaccac gtcagcggtg ggaacgggct actggttcct
     2701 gcagttgctg gacagggttt ccccgtctca gtgggcggca ataggcgtgc tggggagtct
     2761 gctgtttggg ctgctgacat acctgactaa cctgtatttt aaaatcagag aagaccgtcg
     2821 taaggcggcg cggggagagt agacgatgaa taaacaatac gaactggttg ttaaagggat
     2881 aaataattac ccggataaga ttactgttac tgtggcactg gaaattgccg ggcagccgtc
     2941 gttgttgtcg ccatatgtgg cgattagtct tgaccgtact gaaggtgcca cgctggaatt
     3001 ttacgaagct gaggcgaaaa agaaggcgaa gcagtttttc atggatattg ctgccgcttt
     3061 atgtgaaggg gatgaacagt cacgggaaaa gcgcctctgt tcagaagagc ggtataccat
     3121 acaaattaac aatgcatata acacgataca gtcagaaaaa gatgatatcg aatcacgcat
     3181 tgaaaaactg gagaacagcg tcgttgaact gaataaaaaa ctgtcggtgc agatcccttc
     3241 cgaaggtgaa aaaaaacgcc gtgatgagca gttcgcggcg ttttacgatt attgccggaa
     3301 agtgatgaac agaaatctcg cagagtgttt ccgtattcaa aaaaacagag agcgctctgc
     3361 aaatagtgag attctggata attttttaaa ggggacgcat ctcgccagtg attgtttcat
     3421 tgctcattct tttggtggct ttcatgaggc catcaaacat gatgttgaga gaagtccagg
     3481 tatcctctgt tccattcccg taagcatcga aatagacaca aatacgataa ccggtgaact
     3541 cgttaccgcc ggagtcggta tattccgtca tgaggggaac gatgccaaag gttcctttac
     3601 aaaaatcggg ccgttctttc tgaaggtaga gcgatagttt gttaagtacc catccgacag
     3661 ctcgctcatt ttggtttatt ttcgtatcgc gaaacaaaaa ttcgaagcgg ccagatgacg
     3721 catgggtgca accaaatacc ggggcgtatt catcaacctt tggtgttcct gaaaacatgc
     3781 agtccgtgct ctcgaatgca gatgaattgc tgttgatcca cttaacaaaa gaaaaaaagg
     3841 tctgaatcgc ttgtctgtct gaatattcaa caaaatcctc aaggtgtgtc tcgatcagtt
     3901 ccgggtgttt acgaaagtca taatattgac cgggacgata tttatgagtg gttgttttat
     3961 cccatggttg tgctctgcgt ccctgtaaac ggaggtctgc cgctggatag ataagcataa
     4021 gtaatctcct ggtggaatgt aatccacgat catccgggga aattaagaac ccgccagtgc
     4081 ccaccactgg tggggtgaag gcttaacata tccagggatt cggaaccgat aaatcctgat
     4141 aaatatccat gaacaccaaa atcaaatacg gcctgtcggc tgccgttctg gcgctgattg
     4201 ccgcaggtgc gcctgcgcct gaaatcctcg accagtttct ggatgaaaaa gaaggcaacc
     4261 acaccacggc ataccgtgat ggcgcgggta tctggaccat ctgccggggt gccatcatgg
     4321 tggatggtaa gcctgtgatt cctggcatga agctgtcgaa ggggaaatgc gaccgggtta
     4381 acgccattga gcgtgataag gcgctggcat gggtggagaa aaacatcaaa gtgccactga
     4441 ccgaacccca gaaagcgggg atcgcgtcat tctgtcctta caacatcggc ccaggtaagt
     4501 gttttccgtc gacgttttat aaacgaatta atgcaggcga tcgcagggga gcgtgtgagg
     4561 cgattcgctg gtggattaag gacggtggca gggactgccg tatccgctca aataattgct
     4621 acggtcaggt atcccggcga gatcaggaaa gtgcgctggc gtgctggggt atcgacagat
     4681 aagcagaata ttttgctgaa aaatgacgtt tgctcacgcg gacggataac acgaaatcct
     4741 gcgaactgac aaaaactaag tgaataaaag taaaaacccc gtttgttggt cgcaagcggg
     4801 gttgtgtttc tgaccttgga taaggcaagg gagaaattat gggtagggag gtgctttccc
     4861 tgtgaggaag tataaaagat tctttctgag gttgtccatt atgaaaggca ttgaagtgga
     4921 gacgccagcc agtctggatt taacaagagc ggcagctttt gccattcgta ttgtggccat
     4981 tgctgttctg gtctgggcaa tccgttggtg gtgatatgaa taataactct gcaaaaccgt
     5041 ttctcccgtg gttaggaggg aagcgtcgtc tggcaaaaca tattctgccg ttatttcctg
     5101 agcacactct gtatgtggaa ccgttctgtg gcggtgcggc actgttcttt atgaaaaaac
     5161 cgagctgcgc tgaaatcatc aacgatataa acagtgatat cgttaatctc taccgtgtgg
     5221 tgaagtacca ccaggaggaa ttttcccgcc agtttcagtg gatgctcgcc agtcgtgaga
     5281 tgtggaatga ggtgataaat cagcacacgg agtacatgac ggatatccag cgggctgtcc
     5341 gtttcttttt tatccagaag tgcgcgtatg gcggaagagc atacagtcgg gcatttggag
     5401 ttaaaacgca cagaccgcag ataatccccg ggaatacaga gcagttattg caggaaaccc
     5461 gtgatcggct ggcgcgggca actattgaac accttgactg ggaggtctgt atcgcacggt
     5521 atgaccgccc gacaacgctg ttttactgcg atcctccgta ttggttggat ggtggttacg
     5581 gcattgattt ttcattctgc aattatgagc gcatggcaga acttgcccgc acaataaaag
     5641 gcaaaatgat tatatccctg aacgatacgc aggaaatgag acgattgttt gaggggatga
     5701 atattcatca tgttgctcat cgttatactg taccacgtgg taagacaagc agtgtcagcg
     5761 agctgataat ctgcaatttc tgacaggggg catggtgagc ataaaccgaa tgctttccgc
     5821 gtttaccgtt attctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg
     5881 cgataacgcc atcacctaca aagcgcagcg cgataaaaaa gccagagagc tggagctagc
     5941 aaacgcaacc attactgata tgcaggtgcg ccagcgcgat gttgctgcgc tcgatgcaaa
     6001 atactcgagg gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc
     6061 cgctggtcgt aagcgcctgc ggatcaacgc cacctgccac ggtaccgtgc gtgaagccac
     6121 cggcacctcc ggcgtggata atgcaaccgg cccccgactg gcagacaccg ctgaacggga
     6181 ttatttcatc ctcagagaac ggttgatgac aatgcagaag cagctggaag gggcgcagga
     6241 atatatccgc actcagtgta ttaactagta tttttgttat ccggagaatg catgaagaaa
     6301 ttactggtaa ccgtaaagcc ttttcaggga acaattccgt tccgtatttt gcagcgtggt
     6361 cgtgttcttg ttgaaggttc gttcagtggt aaatgtacgc aattacactc ccggaccttt
     6421 caggtgaatg ccacgaatga agagctaacc gttgagtgta cgatgaatgc agctaaatgc
     6481 cgcatggtat cggctgcatt acagccagtg tgttgagcga ccttattatc catgcgcggt
     6541 attgtcgccg tattcctgca ttaacagaga ccgcagcccg acagggagac tcctctgcgc
     6601 gagtgtgcgg ggataatcaa aaacgataca caccggggtt taccgcgtta acggagcgcg
     6661 gagttgtccc ctcatggtcg ctgctccggt gcgatggtgg aagaaaccgg acgatgtgtt
     6721 acctcgcaag ctctgttatg tcatgtgtct gatttgtgat ttaagtcgga taattgtcgt
     6781 tgccattaag cagaggattg atgaccgaca gggtggcatt gttagaataa gacttattct
     6841 tatctgtgcc gggaatgaaa atgaaaagaa atcttccgtt aattattttg ttgtcttctc
     6901 tggttatggg ctgtacgcaa cataaaacag atatgccccg acagttggtt aaggcattac
     6961 cacaatatcc ggcctatgca gtggcaaatt atataaaggg acgggttgat gtgaagtttg
     7021 atattggtgc tgatggtact gtcacccgaa ttgagtttat ccgttcagag ccgcaccatc
     7081 tgtttgatga gcaggttgta aaggcgatgg caaaatggcg atttgagaag gacaggccgc
     7141 gtaaaggcgt gaagaaaacg tttatcttta gtccttctgc accctgatta tttcatcaga
     7201 aattaattat cactctgttg ttattctgta catcccggct gggtaagtct tgttccaccg
     7261 ggtatgaaga tgaaatattg ttggaggaca gtgggtacct gctcctgtaa ccgaacgttc
     7321 atttctcgtt atttgtcatg ctggccggac gcagatgcgt tgcatctgtt gccagccttc
     7381 tcctgcaggc ttcaataacc cacgctgaaa agttaccgga ccctttatgc tcaagggcga
     7441 tgttgatctg ttcaatcatg tgattgggga aacggatatt gcgggttgtg gttctgcggg
     7501 tccggttttt cgatgacata tttatttcct ttactgattg ccatatgacg gggattttac
     7561 atggctgagc ttcgtacact ccagagcaga atcaaaacac tgaatacccg acgggtgaat
     7621 attctgaagg gcgaacagcg tcgtgtcagt ggcagtgcac gtgtttccct caagcgtcat
     7681 atctggctca gggatgccgg gcagtgctgt ctctgtggtc gtgtggttga cctctgtgac
     7741 agtgaactcg atcaccgaat tgcacttcag ttcggtggtg gtaatgagga gacgaatctc
     7801 tggacgctct gtaccgaatg ccatcgacaa aagtctgctc gtgaagcggc gggtggtatg
     7861 ccggacccga cgctgccgga ggtgtccgga ggtagtggca gagcggacga catcatcgga
     7921 ctgtaacccg accggggggg gatcatccgg cgtaaaaaac gatcgctccg gacaccgcgc
     7981 cccctctcac gcagagaaaa aattcccgtt tcagggcagt taacatgtta actggctgtc
     8041 cgggcatttt tgcggttttt atctttatta ttcagtttgt tgcgcggaaa aaatgttaac
     8101 tggctttttc agcaaatgtt aaccaggcag cagttaacat ttgcggcatg agacgccggg
     8161 aaaaatgggc tgaaccatac ccggctgagt gcgttctgga cccgggagga ggctgtgctg
     8221 acaacgcaaa aacgaaaatt tgcgctggcg ctcatgtccg ggaaaaacaa aacagcgtca
     8281 gccattgccg ccggttattc ggcgaagaca gccagggtta aaggctcgca gctggcaaaa
     8341 gatccggagg tgcttgcgtt tatagcccgt aaacagtgcg agacggtgga ggtggatgag
     8401 gttcctgttt accggcagaa aaaatcagag caggaggata aaccccgtcg ccgtgaggcg
     8461 gctgcaatac cacagccgga cgaaacaaat ccggagatgc caccgcccgt ggtgatatct
     8521 cctggtattg agtatatgga ggacggtctt cccgatccgg tgaaagcgat ggggcgtctt
     8581 ctggtggaga acattaatac cgaccccagg ctggcgctgg atgcggctta taagctggcg
     8641 cagttcacgc accacaaaaa aggggatgcc ggtaaaaaat cggcaaaagg tgacgcggcg
     8701 aaaaaagcgg ctaaccgttt tgcggtgcca ccaccacccc gcctggtggt gaataatgat
     8761 aatgagggca acggatgata cctgtgtgga gcacggcctg cccggactgg gcagagcgcc
     8821 tgaaaaaggg gctgtcgatt attccggctc cgatttaccc ggagcaggcc gcacatgccc
     8881 tggcgatttt taaacaactg cggattgtgg atgcaccggg cagcccgacg ttcggggagt
     8941 cctgtgcaca gtgggtgttt gacctggtgg cggccctgtt tggctcctac gatgcgcaga
     9001 ccggtgtacg ccatatcaag gaagttttta tccttatccc gaaaaaaaac agtaagtcca
     9061 cgctggccgc ggggatcatg atgacggcgc tgttactgaa ctggcggcag gcggcgggct
     9121 acaccattct ggccccgacc gtggaggtgg cggctaacgc cttcaaccct gccagggata
     9181 tggtacgacg ggacgatgat ctggatgacc tctgtcaggt gcagacacat atccggacca
     9241 tcacccatcg ggtgacggac accaccctga aggtggtggc agccgatccg aatacggtgt
     9301 ccggtatcaa gtccgtgggg acactgattg atgaactgtg gctgtttggc aagcagtaca
     9361 aagcggaaga catgttacgt gaagccatcg gcgggcttgc ctcccgtccg gaaggatttg
     9421 tggtgtacac aaccacccag tcgaatgaac cgcctgccgg ggtgttcaga cagaaactgc
     9481 agtatgcccg ggatgtgcgc gacggcaaaa ttcatgatcc gcactttctg ccggtgatat
     9541 ttgagcatcc gccggagatg gtggagcgcg gtgagcacct gctgatggaa aacctcgcca
     9601 tggttaaccc gaatctcggt tattcggtgg atgaggcctt tctgtaccgg gagtaccgta
     9661 aagcccggga ggctggtgag gaggcatttc gtggcttcat gtcaaaacac gccaatgtgg
     9721 aaattggtct tgccctgcgt tctgaccgct gggcgggtgc ggatttctgg gagcagcagg
     9781 gcaggcgcgt cagcctggac gatatcctgc agcgcgctga tgtggtgacg gtggggattg
     9841 acggcggggg cctggatgat ctgctgggaa tgtacgtgat tggccgtgac agggaaaccc
     9901 gcgaatggct gggctggggc catgcctggg cgcatgaaac cgcggtggtc agacggaaga
     9961 gtgaggcatc ccggtttcag gattttgtgg cctgtggaga catgacgatt gtccgtcggg
    10021 tcggggatga cacggcggaa gtggcggagt atgtgcgtcg tattcatgag gctgagttac
    10081 tggatcatat cggtattgac ccgtcaggtg tggggcagat tctggattca ctggcggaag
    10141 ccgggatccc cgacggaatt gttgtgggga taagccaggg ctggaagctg ggcggggcca
    10201 tcaaaaccac cgagcgcaaa ctggctgagg gagtgctggt gcatggtggt cagccactga
    10261 tggcctggtg cgttggcaat gcccgggtgg agcctaaagg taacgccatc cttattacca
    10321 aacaggccag cggacggggg aaaattgacc cgctgatggc gttgttcaat gcggtatccc
    10381 tgatgtccct gaatccggag cccaaaaaga aagaatatgc ggtttttttc atataaccct
    10441 gttcaccctg taaccatcat ggaccgctgc ggcggttttt ttattttcag gaggctgatg
    10501 tgactcttaa acgggcctgc tccctgctga cggtgaaatc cttcagtgag gatgagcggg
    10561 tgatcaccgg gattgcgtca acgccttctc cggatcggga tggtgacatc ctggagccgg
    10621 agggcgcgga gtttggcagt gcgatcccgt ttctctggca gcatgaccat tcccgcccgg
    10681 tggggcagtg tacggtgcgc cgggtcagcg aagggctgga aatcacggca acactggtga
    10741 aacccgtacc ggatatgccg tcgcaactgg ctgcccggct ggatgaggtc tgggcggcca
    10801 ttaagaccgg gctggtcagg gggctgtccg tgggcttccg tccccatgaa tacacctttc
    10861 tggacggagg tggactgcat tttctgcgct gggagctgat ggaggtgtct gccgttaccg
    10921 tgcccgcgaa tgcggaatgc accatccgga ccattaaatc ttacgaccgc ccgttttctg
    10981 ccgcgcccgg caaccggaaa ccggtggtga aaatcgcatc ttctgccggc gctgcggcac
    11041 agtcaacaac cgtttttcat aaggaaaaga ccataatgaa tattggcgaa cagattaaaa
    11101 gttttgaaaa caagcgtgca gcactggcag cctcccttga ggaggtcatg accaaagccg
    11161 cagaggaagg gcgcacgctg gatgtggagg aggaagagca ttacgacaac accgcagcgg
    11221 aaatccgtca ggtggatgcg cacctgaagc gcctgcgtga actggaagcc ggtaaggccg
    11281 ccacggcgca gccggtgaaa caggccggta acgggaatgt ggccgcggtg gcttctgcgc
    11341 cggtgatccg tgtggagcag aagctggata aggggattgg tttcgcacgt tttgccaaat
    11401 cgctggctgc ggctaaaggt gtccgctctg aagccctgga agtggcccgt cgtcagtatc
    11461 cggatgacag tcgtctgcat catgtcctga aatcggcagt gggcgcgggg accaccacgg
    11521 atccgcagtg ggcaggcagc ctgtctgaat atcaggaata cgcacaggac tttattgatt
    11581 acctgcgtcc tcagaccatt atcgggcgat ttggtcaggg cgggatccct gcacttcgtc
    11641 aggtgccgtt caatatccgt gtgcacgccc aggtgtccgg cggtgctgcc ggctgggtgg
    11701 gggagggtaa ggcaaaaccc ctgacgaagt ttgattttga atccatcacc ttcagtcatg
    11761 cgaaggtgtc ggccattgcg gtactgacgg aagaattgat ccgtttttcc agtccggctg
    11821 ctgatgcact ggtccgtaat gcgctggcgg aagcggtggt ggcgcgtctg gatacagact
    11881 ttgtggaccc gaaaaaagcg gcggtggcag atgtctcccc ggcgtccatc acccatgatg
    11941 tgaagggcac ggcatcaagc ggtaacccgg atgcggatgc agaggctgcg tttggacagt
    12001 ttgtggcagc aaacctgcag cccaccggtg cggtctggct gatgtccagc accaatgccc
    12061 tggcactgtc catgcgtaaa aatgcgctgg gtcagaaaga atacccggac atgaccctgc
    12121 tgggtggctc cttccagggg ctgccggtga ttgtctccca gtacgtgggt gaccagctgg
    12181 tgctggtgaa tgccccggat atttatctgg cggatgacgg cggcgtggca gtggatatgt
    12241 cccgcgaggc atcactggaa atgcagtctg agccgaccgg cgacagtacc acgccgtcgc
    12301 cggtggagct ggtttccatg ttccagacag gcagtgtggc catccgtgcg gagcgctgga
    12361 tcaactggcg tcgtcgccgt accgcggcgg tggcggtgat caccggtgtg aactacggca
    12421 gtgcgtccgg cggctgagtc tgataaggag gacgggaggc gtgcgcctcc cgtaacaggt
    12481 tatggcaaag atccgatacc tgcagggcac gcatgatgcc cgggccgggg atatccggga
    12541 tgtggcacag ccgtgtgcgg aggtgctggt tcgcctggga aaggcggagt acatcacggt
    12601 gcgacgtccg gcaggtcaga aaaagaaacg tgatgcggag catggcgaat gtggaacctt
    12661 ttacggcgaa cccgaaaaaa ccagaaatca ggacgtgacg taagagaggc gggctggacc
    12721 agcctgtttc aggcggtggc tgagcccttt tccggtgcct ggcagcaggg cgtgaaagcc
    12781 gatcctgaag ccgtcctctc ctttcatgcg gtgtttgcat gtatttcgct gatatcccag
    12841 gatatcgcca aaatgcggct gcgtcttatg cagacggatg cgcaggggat acgcagggaa
    12901 acgcgccggg gggatattgc ccgcctctgt cgtcgcccca atgcgcagca gaaccgcatt
    12961 cagttttttg aactgtggct gaacgccaaa ctgcgtcacg gcaatacggt ggtgctgaaa
    13021 atccgtaatg cccgggggca gatcaaagaa ctgcgcattc tggactggag ccgggttgaa
    13081 cctctggtgg cggatgacgg cgaggtgttc taccgcatca cgccggaccg gaactgcggg
    13141 atcactgagg cggtgacggt gcctgcccgg gaagtgatcc acgaccggtt taactgtttt
    13201 tttcatccgc ttatagggtt gccgccggtg tatgccgccg ggctggcggc cacgcagggg
    13261 catcatattc aggaaaattc gacgtctttt ttcagaaatg gcggcaggcc gtccggggtg
    13321 attgagatcc ccggcagtat tacggaagaa aatgcgaaaa aactgaagag caactgggac
    13381 agcgggtata caggcgaaaa tgcggggaaa acggccattc tgagcaacgg ggcaaaatac
    13441 aaccccacga cgttttcacc ggtggatgcg cagacggtgg aacaactgaa gatgaccgct
    13501 gaaattgtct gttcggtgtt ccgtgtcccg gcctacaaga ttggcgtggg acaaccgcct
    13561 tccagtgaca acgtggaggc gctggagcag cagtattatt cccagtgcct gcagacgctg
    13621 attgagtcca ttgaactgtt actggatgag gcgctggaaa cgggggaaaa cgagagtaca
    13681 gaatttgatg tcaccacgct gctgagaatg gacagtgagc ggcgcatgaa aacgctgggg
    13741 gatgcggtga aaaatacgct tctcacgccc aatgaagccc gtaaacggga gaacctgccg
    13801 cccctggcag gaggcgatgc actgtatctt cagcagcaga actacagtct ggaagcgttg
    13861 tcccggcgtg atgcccgtga agatccgttc gcgtcgtccg gtaaaacagc ttccgtgcct
    13921 caggcggtcg ctgcatctga cggtaataag gcaatcactg aaacagagca tgacgcggtg
    13981 aaggcgatgt tcagggggat tctgaaaaaa tgaatgaacg tgaactgtcc attatccgtg
    14041 cgctgggtga agaattttcc gcggtgctgg cggatttaca gcgcacattt gaggagaaga
    14101 tgagcgcgca ggcacaagcg tttgaagaga aactggcttc cctgtctgtg gtattacaga
    14161 agcatgtgac ggtggatgag gtgcgtccgg ttctgcaggc gatggtggat gacgctgtgg
    14221 gggccatccc ggtaccgcgt gatggtcgtg actacgatcc ggaagtactg cagaaggcgg
    14281 tgaatgatgc ggtcgcaaat attcctgtac cggcggacgg taaaagtctc accccggatg
    14341 atgtgcgtcc gatgcttgaa cagatggtga cagaggcagt gagccatatt c
//
LOCUS       DAZGTJ010000111        14361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000111 DAZGTJ010000000
VERSION     DAZGTJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.111"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..339)
                     /locus_tag="ACHK8E_004312"
     CDS             complement(<1..339)
                     /locus_tag="ACHK8E_004312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346499.1"
                     /translation="MVTTSFRAAETGDTGQAVEAACQPARKPLADWRLPEGMRATLAR
                     RPLPFPFVMCPECFFLMLCFCGYCSSSSSSFRSSPVYGFPGRPPGGAGCRERSQRSCL
                     RPGGLPSLTQK"
     gene            complement(302..493)
                     /locus_tag="ACHK8E_004313"
     CDS             complement(302..493)
                     /locus_tag="ACHK8E_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346500.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(542..967)
                     /locus_tag="ACHK8E_004314"
     CDS             complement(542..967)
                     /locus_tag="ACHK8E_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0346501.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1382..2152)
                     /locus_tag="ACHK8E_004315"
     CDS             complement(1382..2152)
                     /locus_tag="ACHK8E_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346502.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLNAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            complement(2197..2686)
                     /locus_tag="ACHK8E_004316"
                     /pseudo
     CDS             complement(2197..2686)
                     /locus_tag="ACHK8E_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2646..2867)
                     /locus_tag="ACHK8E_004317"
     CDS             complement(2646..2867)
                     /locus_tag="ACHK8E_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346503.1"
                     /translation="MTNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPER
                     LVRQTLFRASYLFMKAYNEILELDYCFKD"
     gene            complement(2864..3547)
                     /locus_tag="ACHK8E_004318"
     CDS             complement(2864..3547)
                     /locus_tag="ACHK8E_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP0346504.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(3645..3956)
                     /locus_tag="ACHK8E_004319"
     CDS             complement(3645..3956)
                     /locus_tag="ACHK8E_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346505.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASQSPFSDERPSSRFRGITLPSERRRLRYSTVGLTRYRT
                     R"
     gene            complement(3953..4855)
                     /locus_tag="ACHK8E_004320"
     CDS             complement(3953..4855)
                     /locus_tag="ACHK8E_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0346506.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRCATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNICSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(4963..5247)
                     /locus_tag="ACHK8E_004321"
     CDS             complement(4963..5247)
                     /locus_tag="ACHK8E_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346507.1"
                     /translation="MLRPFFFSDKGCRRAPLFTAPADAARQERCLWSVSRVRPARDGR
                     FYRSRLASVYASQSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRTR"
     gene            complement(5271..5693)
                     /locus_tag="ACHK8E_004322"
     CDS             complement(5271..5693)
                     /locus_tag="ACHK8E_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0346508.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKAILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            complement(5696..6676)
                     /locus_tag="ACHK8E_004323"
     CDS             complement(5696..6676)
                     /locus_tag="ACHK8E_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0346509.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ANIPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRAVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQREQILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            7630..8265
                     /locus_tag="ACHK8E_004324"
     CDS             7630..8265
                     /locus_tag="ACHK8E_004324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0346510.1"
                     /translation="MTYLSTVGHRFIMDHARLNPVLDWVGIPIDGIDSLVNSEIRSDK
                     MSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLSDIVCWVDSLPYKIQAWRFY
                     QFCPLGVGKEKRDKLEINTDIFKEKMVMLKKQHPNMPISWATFEERDKANVVMEPNFD
                     IIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKNNRFWLDKMEFTV"
     gene            8262..8969
                     /locus_tag="ACHK8E_004325"
     CDS             8262..8969
                     /locus_tag="ACHK8E_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP0346511.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYEAYMVLWSRINELLTEPDIIAFINYPVAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            8971..9396
                     /gene="nudI"
                     /locus_tag="ACHK8E_004326"
     CDS             8971..9396
                     /gene="nudI"
                     /locus_tag="ACHK8E_004326"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0346512.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(9890..10015)
                     /locus_tag="ACHK8E_004327"
                     /pseudo
     CDS             complement(9890..10015)
                     /locus_tag="ACHK8E_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10015..10302)
                     /locus_tag="ACHK8E_004328"
     CDS             complement(10015..10302)
                     /locus_tag="ACHK8E_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP0346513.1"
                     /translation="MCKIKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQ
                     ALSAWLAQEEERNRLTLEALDDVTYGQVIDHQAVQARADSLSTDNPLPVPR"
     gene            complement(11257..11571)
                     /gene="pspE"
                     /locus_tag="ACHK8E_004329"
     CDS             complement(11257..11571)
                     /gene="pspE"
                     /locus_tag="ACHK8E_004329"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0346514.1"
                     /translation="MFKKGLLALTLMFSLPVFAAEYWIDVRIPKEYQKEHVQGAINIP
                     LKEMKERITTAVPDKNDTIKVYCNVGRQSEQAKEILNEMGYIHVENAGGMKDIEMPKV
                     KG"
     gene            complement(11739..11933)
                     /locus_tag="ACHK8E_004330"
     CDS             complement(11739..11933)
                     /locus_tag="ACHK8E_004330"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0346515.1"
                     /translation="MNQDKANYLANKYFSPANEAVRLEREGDYIEAWKKWSEALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(12632..13360)
                     /locus_tag="ACHK8E_004331"
     CDS             complement(12632..13360)
                     /locus_tag="ACHK8E_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346516.1"
                     /translation="MKNTLLPLTLAFILSGCSNWVNGNKILADKNITEQQINSQIENN
                     KTSINEVNMLFGDKKQEGRSVIKKTFPNGTYNIVSYQGHLNDLGGTYAHRVLFVVYDK
                     DGVVINHDIAINNFREKNKFEQRPDELRLTAFNELNKNDSKSKVLNLLGEPRTLSFSD
                     SGNVLWVYSNTEISRDASSYIPVFNMIKGTESGMSERVYIELKDNKVENIYLASFKIV
                     QGRGATNAADYKEDVIDIRKKYEL"
     gene            complement(13684..14151)
                     /locus_tag="ACHK8E_004332"
     CDS             complement(13684..14151)
                     /locus_tag="ACHK8E_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346517.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRSGSQWLVVEVF
                     GEKGRQHRSADSNPDMVEKMAARYRHAAGQHWSATLAEIERVKRIYQARIKAS"
BASE COUNT         3823 a         3598 c         3298 g         3642 t
ORIGIN      
        1 tttttgcgtc agggatggaa gcccgccagg gcggagacag ctacgctggc tccgttcacg
       61 acagcccgcc ccgccagggg gacgcccggg aaagccataa acaggagagg aacggaaaga
      121 ggaagaagaa gaagaacagt aaccacagaa acataacatc aggaaaaaac attccggaca
      181 catcacaaac gggaagggta acgggcgacg cgccagcgtg gcccgcatcc cttccggcag
      241 gcgccagtct gccaggggtt ttcgcgcggg ctgacaggcg gcctccaccg cctgccctgt
      301 atcacctgtt tctgctgccc tgaaggatgt agtgaccacc gaggaaaacc gcctgctgat
      361 tcgccacaaa atcgcagatt tcatcaacaa tctgtgctgt ggtgagcggt ttctgcccca
      421 tttcacgacg ccgcgcatta atcacggccc tgtaattacg cagaatttcg cgggtttctg
      481 tgctgatgtt catttttctt cgcctccttt acaggggcgg catcgccgcc ccgtccgtta
      541 atcagtccag tgctgcgtaa atcgcattgc attcggggtg aaattcgatg tggctccaca
      601 actgcgcatc acgcatccgg tacaggtgtg tcagtcccgc atccccgctg tcgtggtaca
      661 gccacaactg gcggttaatc acaagggaag tcaggatgat acctgcggca tcagcactca
      721 cagtgcggtc aaaccagttt tcaccgttga ccagatgaaa acggtcgcca tccggcatca
      781 tgtaaccgcc accgtccggc aggcggatga aatgccagaa gcctccctga tattccggca
      841 tcattttttg cgccagcgcg tacacatacg cctcggcata cagaaaatcg ttgccaaaca
      901 ggtaaggcag aaaagaaagg cggtcgcact cttcaacatt cagagtgaca ggtttcgcat
      961 actgcatggg atttcctccg ttgttcacat attccgcgac accgggcggt gccgtgcctc
     1021 cataaaagcc gggcgataag gcggggatgc acagggcgca agggaagccg cagcctgacg
     1081 cacacggctg acgggagttg gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga
     1141 aaatcacggc agacgggaag gagggaccgg gtgaccgccg cgtgcatttt acccttgcat
     1201 ccccgccagc ccggctaaga tggaggcacg gcaccgcccg catacaacgc tgaggccaca
     1261 taactgaaaa cccacaaggc catgccccgc atggccgggt aaagcggagc ctgcggcgac
     1321 atccttttgc tctgtccggg gaccggcagg aaaaggtgga gatttttccc cgccagcgtc
     1381 atcatttccg gattttcgcc agtgcatccg ccatgctgcg gatttcatcc cgtgcctcca
     1441 gaacaacctc ccgcagaata tcccggtcaa accagcgacg ggacggttca tccggtatac
     1501 gcagcacatc acagaccaga ccactggcca ggatatgctc cacacactca cagaccaggt
     1561 acatttccac agaggaaaaa cgggcgccac ggttcagaat ggcattgagc accacgggca
     1621 gattttcgtc atcttcatca agccagcagg ggtaatcgct ttccttcaca accagacgaa
     1681 agcggcgcgt ctggcgggtc agcaacgtct gaccggtggt ggtcagcgcc gcccggatac
     1741 gccgcgcatc gtctgccacc tggtaacaga tgcttcgtac tgcattgcgc accgcatcaa
     1801 gaactgccct gatttcttcc tccgtcagcc attcaccggc atacgggaaa gacacctgcg
     1861 ttccgtctgc cggaagtacc ggcagcgcct gccactggct gccggacagt acaagttccg
     1921 ccacggttcc ggtcagtttt tgcatggcag acgccaggac cggcggcagc acgccatcat
     1981 ggcggctgat aagtaacgtc gccagccggg atacatccag tcgcacataa atgctgatcc
     2041 agcccggata gtgcggattc agttgttcaa tatccgtctc ttccggccag ccctgcgcca
     2101 gcagcaggtt acgggcgtgg cgacaaacca gtgcattcag cgttttgttc atgattgatg
     2161 gtctccctga atcgccgccc gaaggcggcg ataacatcag tcgttttcgt gtgtgttgtt
     2221 tttcagcagg atgcgggcct gattaaccac cgcctgacgg cgggtaacgc attccgggac
     2281 ggcgaggccg taatcacggg cagcccattc acgtttccac agtgcctgtt cggcggtctg
     2341 aatcagggac gccagcagtt cagccgggac ggtcacctgg cggttttctc tcacgttatt
     2401 gatgatctcc atcgccacgc cggaagaaat accggactca gtccgctggt cttccgggat
     2461 gtcctccagg cgcgccagga cggtcctgat ttcatgatcg gacagggaaa tatccattcc
     2521 gtcagcgagg gcctgaatct cttccgggga ccagataacc agcatcaacg gcgtgtctcc
     2581 cggatacttt gctgccagcg cccggcatag cgtttcgcat gttccgtaca tactcttctc
     2641 tcccctcaat ctttgaagca gtaatccagt tccagaattt cgttatatgc cttcataaac
     2701 aggtaactgg cacgaaagag tgtctggcgc accagacgtt ccgggagggt gtccgcatcg
     2761 ctgtaccgct gctccatctc attcatggtt gtacgcaggt cgcacatggt attggccagt
     2821 tccgccacct gttcacgcat atcttcgaat ccgctgtaat tcgtcatgca gcctccggtt
     2881 caaaccagtt atcattcgcg gccccctgct gcatggcccg ttgcacggcg gccagtcgtt
     2941 gctgtccggc acggtgatac tgttcaagca gctcgatacc gatataccgg cgtccggact
     3001 gaagggcggc cacgcaggtt gaaccgctgc ctgcaaacgg atccagcaca attgcgttcg
     3061 gatgtgtgaa gctctcaatc agcggttgca ggctggtaac gggcttttcc gtcgggtgat
     3121 ggcggttgcc cgaatatttc cagcccagca catccggcag cgggttacgg ggccgggccg
     3181 gactgccctt tgccaggatg taagcgcatt cgtggcggta cgcgacatac gcagatttgg
     3241 atgtgtagtt tttggtgaac acgaggtgcc ctgccacgct gaatccggcg cgtttccagg
     3301 ccgccataaa gcggtcaata cggtgccagc cgtagaagct gaccatcagc gcgtcttttt
     3361 tgagtacgcg gtacatctca ttgctggcag gttgcagcca gtcatcatta acgtcaccag
     3421 caatggtgcg cccggaacgg tcacggaaac cgacgagata tggcggatcg gtgaggatga
     3481 aatcgacagc attgtccgga aacgtggcca taactcgcac gcagtcaccc aggataaaac
     3541 gggacataaa agcctccttc tcttacagag ggcaccggga gggcaccggg agggcaccgg
     3601 gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg ctgttcagcg cgtccggtaa
     3661 cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt ctgacggcaa ggttatgccg
     3721 cgaaagcggc ttgaagggcg ttcatcagaa aagggcgact ggctggcgta cactgtcacc
     3781 gacgccagcc gggagcggta aaagcgcccg tcccgtgccc gccggacgcg ggacactgac
     3841 cacaggcagc gtttatgccg cgccgcgtca gccggcgcag taaaaagagg agcacggcga
     3901 catcctttac cggaaaagaa acgggcggtt gcccgcccgt ctccgtcata cttcatccgc
     3961 cataatgcgc caccttgtcg actatccacg caggtccggt gacttccggc aactcgtcat
     4021 catctgtcgg atgtgaccat tccagctccc cccacagcac atcaacaagc tctccacgct
     4081 catatcgctg ccagccacag aaattgcagc cctgttcggc ataccagtgc tccagtgtgc
     4141 agctgaaacg gcggcttaat tctgcaataa cgtcgctttc cggctgacac cacggcgtgt
     4201 cgaaatcaac ctgaatgaag ttgtctccct gactacagat attcaggtca tacccacaag
     4261 gccacttcac gccataccgt tctgtatacc agtgatatgc agaaggaacg ccgttcagaa
     4321 caccaccgtt aaagccattc acttcaacat ccaggcgggt cggcagaacc gtcagcaggt
     4381 caaacggcat ggttttttcc ggcactacgt tgtcacacag acggttccac cacaccgata
     4441 catcctcgct accccagatg tcacaccagt cacctctttt tgcggtgaaa tgcacagtga
     4501 tggtttccct gacatcatcc ggtaatcctt cccagcgacg ctgtccagta ccgctctgca
     4561 gccagagttc atgcagcatc aggcagtttt gctcatccag cagtacaccg ttctgcaggt
     4621 gtgtcagcca gcgggtgaac gcgatatttt ccgtcgatac agcaccacgt ccggcagccg
     4681 tcactccggg gcactgctca gagcggatat tttcagtgat ctgcagaagt ccggcacttc
     4741 cggccagaaa cagctgaata ccttcatttg tggcgcagcg ataaaacggt gtgacggcac
     4801 cactggccag tcgtttaatc tcagcgatct gtgccggttc accagaaaaa tgcatacggt
     4861 tactgcacca gtttggcatg ggaattctcc tctcacttct gcttcagcag agagcaccgg
     4921 gagggcgccc tgtgcaccag ttccccgctg cctgtgggct gttcaccgcg tccggtaacg
     4981 ggtcaggccg accgtggaat accgcagccg tcgtcttttt gacggcaagg ttatgccgcg
     5041 aaagcggctt gaagggcgct catcagaaaa gggcgactgg ctggcgtaca ccgacgccag
     5101 ccgggagcgg taaaagcgcc cgtcccgtgc cggccggacg cgggacactg accacaggca
     5161 gcgttcctgc cgcgccgcgt cagccggcgc agtaaaaaga ggagcccggc gacatccttt
     5221 gtcggaaaag aaaaaaggac gcaacagcgt cctttaacag agttcagttc ttattcatca
     5281 tccggaaaca agttcttaat gttgtttctt gtttccagga gattctgatc gactggagca
     5341 gatatttctt gcgtagcaga atcaaccgca ggcaggatcg cttttgcatt attaccggca
     5401 gactgcagaa atgcctccag caaacgaaca acttcaccat tttcctgttg tggtagcgta
     5461 ctactgataa ttcgttgaat atcctgcata gacgtgctgt ctgtcaggaa ttcagctatc
     5521 aggaacggca atcgttcatc ctgtttcatc aatgcagcac cacacaacat catggcccgc
     5581 attgcccgac cacgttcttt ctgaggcaat ttttcaagaa acctgtcagc cacccgatca
     5641 gctgcatttc gttccggata aatataaaaa gagcacttac gattatcagc catactcatc
     5701 ccttcattgc catcattccc aacaccagat cgaactgagg attgtcactg acaaagaaac
     5761 ggttatcacc cactcctgtc gcttttttta ctgccccagc cacaaggctg gctccaccgc
     5821 caacaaccat aacatgagta aaaccagcaa agcgtcctac tgaatccgcc acacgatgct
     5881 gcagtttttt ttcccggtca cgtaatacag tcaggatctg ctcacgctgt tctgcattcg
     5941 ggatataacg agtcaggaat tcattgtcat cccggccctg gattagacga tctgcaaaat
     6001 aagaactggt tcgtgtactc acattcacat ccatcgcatg cttaactgct tcagtaatga
     6061 tggaaacgcc tgtcttggga tcacaataag tttttgtgaa acctgacatt ttgctgcgga
     6121 catgtgcaat atcaagggtc gttccaccga tatcaacgat caacagcgac tccagatcat
     6181 tcagatcttt cagcacgtca aaaccggcag gcacggactc aggcaagaca ctgactttac
     6241 gaatggtaaa gttttcacgc ccctgaactg ttacagctcg tctgacatta tttttcttac
     6301 gttcgatatt agccagattt ggctgatcgt tctcatccag atactcacct aaaggtaaag
     6361 tcacaacaat atcaacaggt tgcggagcaa tacctgactg catcaacgca tgatgaatag
     6421 caaccacatt aacgtcgctg tactgataac gcgtttctgt ggtgcgcact gcatccggac
     6481 tcaattgatc aaagctgtat ttctctccat caatctcata gtttgcggaa gcgggtatat
     6541 tggcatcagt cagtccctgc agcgtcatag accattccgg cttaaaacta ttcggactaa
     6601 ttaacgtttt cactgcaccg tcttcaaccc aggccagttt gatgttggta gaaccgtcat
     6661 ctacaaagat acgcatccag aacctcacag aataaaatgg atttttttag aaaaatatgg
     6721 tgtcgaaaac gattatcacc taaaaacacg catcagtaaa gacctgatat cggccacaaa
     6781 cagaaaaata aagaaaaata aagacaaaaa ccgactcaaa cagcatacca gggaacaaac
     6841 aagaacaaca aaagataaat acagttaaaa tcaagaaaaa actgaacagg tgaccggaga
     6901 aggggttctc atccagcaca tgctgtatct ggggtcagtt tggaattcgg aaaataaacc
     6961 acgttaagac tattttttag tcaaataact ttcaggaatc gccagaatgg cgggacaaca
     7021 gcggttttgg tgtccgaaat cgtacaatta tgtacatcta cgcaggggaa tgtacggaag
     7081 taagccaaac agttatcgga cggtcggaac atttcgtaca gcctttacgt tcggtgagtt
     7141 agctggcttg gatgtcacct ctcgttcaac tcaattctct tagcgtggtt tattttccga
     7201 attccaagcg gcccaatcat gggcttccag taatgcgatt accggaactc atcattcatg
     7261 agttccttgc gcaacccact gatctgatta ctggcagata ttatatccct ggtaagccga
     7321 aggttgcgct ttgtcatggt ctgcaataac cattatgttt taatggttat tgatgtatca
     7381 tagtcagagg acttgttatg gatattagca cccttctttt agatttcaga ataacttcac
     7441 attgtaacct aggatgtgat ctgtgttttc gtacacctgg aattcaggac tcttctcttg
     7501 ctagtgctcg taatgtaatt gagcggatgt ataaaatggg attccgtcgt atcggtttta
     7561 ccggagggga acctacatcc cggatgtgat ttcattgatc tgattgaata cgctaagcaa
     7621 ctgggtttta tgacatatct ctccacggtc gggcatcgat ttattatgga tcatgctcgg
     7681 ttaaatccgg tgttagactg ggtaggaata ccaattgatg gaattgacag tcttgtcaat
     7741 tcagaaattc gcagtgataa aatgagtaac caacacaggg ttatcaaaaa tatattaaca
     7801 tggttacccc aaaataataa tcgaattaat ataaaactca cgaccgtcgt atctcagtct
     7861 aacattaatc atctcagtga tattgtttgc tgggttgatt ctctgcccta taagatccaa
     7921 gcttggcgat tttaccagtt ctgtcctcta ggagtcggaa aagaaaaaag agataaactg
     7981 gaaatcaata cggatatctt taaagagaaa atggtaatgc taaaaaaaca acaccctaac
     8041 atgcccatta gttgggcaac tttcgaagaa cgggacaaag ccaatgttgt tatggaaccc
     8101 aattttgaca ttattatccc tgatggagaa aactatactc tgttgggaaa tatgctgaca
     8161 gattcacaag atcatataat cagtgcaatt ttcggaaata atgaaatcat gcaaaaatgt
     8221 aagaataacc gcttttggtt ggacaaaatg gagtttacag tatgagatat aattacttgt
     8281 atatggatcc ttggtcagca gataaaaaag agaagaataa aactagaaat cctctgtata
     8341 tatgtctgtc gggtaattcc ggtgtaggta aaagtactct tctgagacgc atgtcaaagg
     8401 agttatttaa ggtagatcca gacactgtcg cgatagatga aaaaagtgtc caccacactc
     8461 tccttcctta cctttttgac gataccagta actacggtta tctaatccag cttaatttta
     8521 tgatacaacg agcattactg ataaaatctt ggctggataa agggtttaac cttattatgg
     8581 agagatccca tattgaggat tacattttta tcaacttcat gctgaagtcc gaatatataa
     8641 ataaagcaca atatgaagca tacatggtgc tctggagtag aataaatgaa ttacttacag
     8701 agccagacat tatagcattc attaattatc ccgtcgcaca ttcactcatg catctcaatg
     8761 atgatgaaat tcatggtgta cggcctgaag aatttccaga tgaggaaatg aagataaaat
     8821 ggatcacagg atggcatgcg gaatatcaac attttactga taatcttccg ttacaccttc
     8881 gtgagcggat tatggtatgt gctcacccgg atgacattga tgatttttct gataaggtta
     8941 tccaaaaagc catgatgttg aggacataaa atgcgccaga gagtaatagt gtgccctgtg
     9001 atacaaaatg aaggtgagtt tctactttgt aaaatggcct ctgatcgtgg agtattcccg
     9061 ggccaatggg cactgcccgg cggaggaatg gaacccggag agactatgga aacagcattg
     9121 aggcgtgaaa taaaggaaga actgggaaaa gaactgcata tcactgacgt aaaaccctgg
     9181 gcctttcgtg atgatatccg tacaaaaacg tacccggatg gtacccacga ggacatttac
     9241 atgatttacc tcatattcga ctgtatcagt acaaaccgta cagttacatt taatgaggag
     9301 tttcaggata tagtctgggc atctcctgac accctaaaat atatggattt aaatgaagca
     9361 acccggatca cattttctca gaaaggtatc ctttaaactc caatgctaat cgtgcaactg
     9421 ataacggctt ccacaacagg gagccgtttt cttatgccac tactcaagga tggggtttga
     9481 gggccaaccg tacgaaaacg tacggtaaga ggaaaattat cgtcttaaaa atcgattagt
     9541 agacaatgcc gtccgttaag tgccaatttt cgattaaaaa gacacagttt tgatggcgtt
     9601 tttctatgtc cattatgttt tgatatatca gacagttact tcactaacgt acgtgggctg
     9661 cgtttgggaa acgtacaata ttgtacggtt gcgctattgc ggtagggtgt actcccgtgc
     9721 tatacggcgt tttcagagga tttgacttta atcggatcac ctaaatgcga taccgaaaga
     9781 gcgacgactt aaaagcgata gccgctgact caaaatcaat ccgttacgct tctcgattga
     9841 ttaaataatg cgcttaacgt acaaaatttt ccgatatcca aactgacccc agaatgaccg
     9901 acgcctgtgt cagggactgc actgttctgg ctgcaactgg tttactagcc agcgccagaa
     9961 aatcatataa ccgtgccaaa tcagaaagcg ccttactggt ccacttcagt tccatcagcg
    10021 aggcaccggt aacggattgt cagtactgag gctgtctgcc cgggcctgta cagcctgatg
    10081 gtcgataacc tgtccgtatg tcacatcgtc caaggcttcc agagtcaggc gattacgctc
    10141 ctcctcctgg gcaagccatg cagaaagagc ctgtttgata atccagcccc gggagcgttc
    10201 cagtctggcg gccatctggt cgactttatc agccataggt aatggcacat gcgcggtaat
    10261 tacttttgtt gcctgtgcag tattgttttt aattttgcat atccgattag ttgttaatca
    10321 catttaatca tattgattat attcagacag aacaaggcaa ataatgcgcg tcagcgcatg
    10381 taaagcagca cgcagtggtt atgtgtgaca ccggagtaaa accgccgcag cctagcgtaa
    10441 gccgggatgt cagtagttct ccggtgctga cgcgggacat agaacgggtg ctggagtgaa
    10501 acggggaagc tcaggtatac gggccacgcc tctgggcaga ttctgctccg caacagtatc
    10561 ggcgtgcctg acctcctgag agctgtaacg aattttctcg cctcccatgc cctgacgcca
    10621 ttgagcgcgt tacagaacga ttgaggtcat ttaacgggac tccaacggct cgaccgaaat
    10681 agataacatc ttgatcgtca acgatatacg agaggaaccc ccgctcgccg caagtcgatg
    10741 accgaacgta gtgagcggat cgacgaggaa gcggaagaga acctgtacga aaatggagca
    10801 cttacgcacc accgtaaata attaagaaat ggatctcctg caacctacat gaatgcgaag
    10861 tgcgagtatg gcgtggtccg tcaatgggga tgtgttttca ttgagttata tatccgtcgg
    10921 ctatcttatc accagtttcc gtctgtgttc attatcttaa aaatattctg ccggaacagg
    10981 attacggtta gccacaagct tcattgcaac ggcagcaacc tgaacaaaat gacgacggtg
    11041 ggtatgcctg aaagcgtgcg aattgcctga aaacccaacc agatacagag agacttaccc
    11101 gaaatctgac ttattcaaca aaacctcaac ctttctaacg ttccagcgaa aaaggattcg
    11161 gcggtaaaac cgccgaaatt tccggtataa agaattaatc taattgttaa gcacttttgg
    11221 cgtgtactga tagtgaaatt ttttaacctt taacctttaa cctttaacct ttggcatttc
    11281 gatgtctttc atccctccgg cattctcaac gtggatatat cccatctcat tgagaatttc
    11341 ttttgcctgc tcagactggc gtccaacgtt gcaataaact ttaatagtat catttttatc
    11401 aggaactgcg gtagtaatac gctctttcat ttccttcaga ggaatattta tagccccctg
    11461 aacgtgctct ttctgatact cttttggaat acggacatcg atccaatatt cagcagcgaa
    11521 tacgggaagt gaaaacatca gtgtcagagc aagtaagcct tttttaaaca tgtaactctc
    11581 ctttaattga tgattatcat ctataatatg cttgatgttt aataattttt gcgaatcact
    11641 tctcacattt tctacttaag gcgtattcgg gttttcaaaa gggtgcccct ttgatacgtc
    11701 gaccggccaa agggaagcac ctttgaatcc cgtcagaatt aaaaccgacc tgttttttta
    11761 caatattcag ctctggctat acaccaacct atattttctc ctttggcata atgaagtgct
    11821 tcactccatt ttttccatgc ttcaatgtaa tcaccttcac gctccagcct gactgcttcg
    11881 tttgcgggtg aaaaatattt atttgctaaa taatttgctt tatcctgatt catacacgca
    11941 tttatattta cggagttaaa aagagagagc cagtcgggtt aatacatatt ggcctgatgt
    12001 tgatatatca gcccaacagc acgctcgtaa ttgttttaac gtatttttca caacatggca
    12061 atatggatgc atctgttcgg taatcaataa aaaaccgtac gaaaaacggg acaggatgcg
    12121 tgttcgccgg accgcgcacg cacaatcaga caaacgccac caccctgaca ccctcgacag
    12181 cgttcctgca atacgatata acatcagagc gcgtttccgg ataaacacga gttaatgacc
    12241 gggaattcat tgattaggga ccggagacgt gcttaaaacg tttatggcgg cctgaaaatt
    12301 aaatacttgc ctgaagcaaa aaatcgcgtt acaaagcgtc tgagcgcgtt tctggcgtct
    12361 ttttttgttg ctgtgtcgtt gtacttacct tgttgtcaga ttcgcgctca atcatcactg
    12421 atcaatctgc gcttatcctg ctggtatttt tttacaacca acgcccacga aaatgccaga
    12481 cgggaaccat tatccatgtc ctctgccatg ccaactacag ggggattaca ggcaaaaaat
    12541 cccccggaac tgatgaaggc cagaccaggg agagcaaatg gccaaataat ttttgtataa
    12601 aaaacacgat tatattttac ttggtaaaaa tctataattc gtatttcttc ctgatatcta
    12661 ttacatcttc tttataatct gctgcatttg tagccccacg gccctggact attttaaatg
    12721 atgccaaata aatattttca actttgttat ctttcaactc aatgtaaacc cgctctgaca
    12781 ttccagattc agtacctttt atcatattaa aaactggaat gtaggaagat gcatctcttg
    12841 atatttctgt attgctgtaa acccacaaaa catttccaga atcagagaat gataaagttc
    12901 ttggctcccc aaggagattt aatacctttg atttgctatc atttttattt aattcattaa
    12961 aagcggttaa acgtagctca tctggtcttt gttcaaattt attcttctct ctgaagttgt
    13021 ttattgcaat atcatggttg ataactacac catccttatc ataaacaaca aagagaacac
    13081 gatgagcata agtaccacct aaatcattca aatgtccttg ataggatacg atgttataag
    13141 taccgttggg aaacgtcttt ttaattacac ttctaccttc ttgtttttta tcaccaaata
    13201 gcatattaac ttcatttatt gaagttttat tgttttcaat ttgtgagttt atttgctgct
    13261 cagttatgtt tttatctgca agtattttgt tcccattaac ccaatttgaa caaccagata
    13321 aaataaaggc aagtgttaat ggtaataacg tatttttcat aaaaatatcc tttttaaata
    13381 gagaaaactc aataaagtga acaaacatca ccactttaat gttatctgta atcacttaca
    13441 attaaaaggg gttagtttgg agatcggaat gttttgtacg ctaagagcat tataaccaat
    13501 cgcgatgcgt aacagactga tttttcgcca actgatatca attttaaatt gtcgctcttg
    13561 ctgtatcgca tttaggttat ccgatgaagt tccagcgtag aacctcatcc aagatgtggc
    13621 cattaatgct aatgtatgca tacattagca ttaatggccc agacaaatac caggcatccg
    13681 gtctcaggat gccttgattc ttgcctggta gatgcgcttt acccgttcaa tttcagccag
    13741 cgttgcggac cagtgctgac cggcggcatg acgatatctg gctgccattt tttccaccat
    13801 gtccgggtta ctgtccgcgc tgcggtgctg acgccccttc tcaccaaaaa cttcaacgac
    13861 cagccactgg cttccgcttc tgccgaacaa tgccttacac aaacgtctca cggtcagcgg
    13921 ggttaaatct ggatcgggat catatgcaat ggtagcaaaa ataaagcgca gcatttctga
    13981 tgtcttgtaa cgctttacgg cagctgccag acgtgcggcg cgttgaggtg cttcccagcg
    14041 gtcgtcactg taattagcaa cttcagccag ataatccttt tcaaggtttt cagccgtgta
    14101 acacctgccg cctaaaaaaa accgcatggc ctcccgggtt ttgtctctca tatatcatcc
    14161 ctcattaatg ctaatgtatg catacattag cattaatatc tgacagcaac aagcgacgga
    14221 taattgccag aattgcaggg ctggagatac atttggataa cgtaatgatc gcagtataca
    14281 ggctaggctg tcaggttttc agctgtatct gttcctgcag cagctccaga tgatatacag
    14341 caatagcatt taccacccct g
//
LOCUS       DAZGTJ010000112        14186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000112 DAZGTJ010000000
VERSION     DAZGTJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.112"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1854
                     /locus_tag="ACHK8E_004333"
     CDS             <1..1854
                     /locus_tag="ACHK8E_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0346518.1"
                     /translation="RITQKLKEGWQPYGSALISTAGYGAEFIQPVVSEGSISSPEEPG
                     NRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTFDSPDPRIKQLARRSTVTPG
                     GAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGCVGQGLHIAKKLLPFIPANA
                     GILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGRPLYKDLIGRTKAALAKNPK
                     NVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLADMAGQCVGGSADSVPWICGD
                     TTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPDIGY
                     YGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAILSCAGKSSAFVNGTAGVVVP
                     DRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVDDSGNKALRIEKNNSAKSWS
                     MYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKYAFGLYWRIAEWPGEGGEGY
                     LSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHTLAFKFKGSNSINVTPVLDG
                     VDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDTVEVKANKAGAAA"
     gene            1994..2176
                     /locus_tag="ACHK8E_004334"
     CDS             1994..2176
                     /locus_tag="ACHK8E_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0346519.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            2202..2474
                     /locus_tag="ACHK8E_004335"
     CDS             2202..2474
                     /locus_tag="ACHK8E_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0346520.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            2551..2766
                     /locus_tag="ACHK8E_004336"
     CDS             2551..2766
                     /locus_tag="ACHK8E_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0346521.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            2771..3304
                     /locus_tag="ACHK8E_004337"
     CDS             2771..3304
                     /locus_tag="ACHK8E_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0346522.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            3301..3768
                     /locus_tag="ACHK8E_004338"
     CDS             3301..3768
                     /locus_tag="ACHK8E_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0346523.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            4071..4877
                     /locus_tag="ACHK8E_004339"
     CDS             4071..4877
                     /locus_tag="ACHK8E_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0346524.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            4858..6564
                     /locus_tag="ACHK8E_004340"
     CDS             4858..6564
                     /locus_tag="ACHK8E_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0346525.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            6564..8708
                     /locus_tag="ACHK8E_004341"
     CDS             6564..8708
                     /locus_tag="ACHK8E_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0346526.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            8866..9873
                     /locus_tag="ACHK8E_004342"
     CDS             8866..9873
                     /locus_tag="ACHK8E_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346527.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            9897..11111
                     /locus_tag="ACHK8E_004343"
     CDS             9897..11111
                     /locus_tag="ACHK8E_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP0346528.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            11167..11556
                     /locus_tag="ACHK8E_004344"
     CDS             11167..11556
                     /locus_tag="ACHK8E_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346529.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            11606..12067
                     /locus_tag="ACHK8E_004345"
     CDS             11606..12067
                     /locus_tag="ACHK8E_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346530.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQESVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            12051..12614
                     /locus_tag="ACHK8E_004346"
     CDS             12051..12614
                     /locus_tag="ACHK8E_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346531.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            12614..13264
                     /locus_tag="ACHK8E_004347"
     CDS             12614..13264
                     /locus_tag="ACHK8E_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP0346532.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            13261..>14186
                     /locus_tag="ACHK8E_004348"
     CDS             13261..>14186
                     /locus_tag="ACHK8E_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP0346533.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAITAAGNAEESSTRAGESEKAAGADAEKARLHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIP"
BASE COUNT         3662 a         3276 c         4065 g         3183 t
ORIGIN      
        1 cgaataactc aaaaactgaa ggaagggtgg cagccttatg gtagtgcgct gatttcgaca
       61 gctggttatg gtgcggagtt catccagcca gttgtgagtg aggggagcat ctcatcacca
      121 gaggagccag gcaaccgtcc gacgacctca gcgccttctg ttgcgccaga atattactat
      181 gtgatcgcgc ttgctggtca gtccaatggt atgtcatacg gtgagggact gccattaccg
      241 gatacattcg acagccctga tccacgtatt aaacagttag cgcgtcgcag tacggtgaca
      301 ccgggaggtg ctgcatgtat ctataacgat attatcccgg cagatcactg tctccatgat
      361 gtggaggata tgacagggct taatcatccc aaagcggatt tatcgaaagg tcagtacggc
      421 tgtgtgggac aggggctgca tatcgccaaa aagcttctgc catttatacc ggcgaatgcg
      481 ggcattcttc ttgttccgtg ctgccgtggt ggttcagctt tcaccaccgg aaccgatggc
      541 acatacagtg acactactgg cgcctcagag agttccaccc gctggggggt gggcaggccg
      601 ctgtataagg acctcattgg tcgtacaaaa gccgcgctgg cgaagaatcc gaaaaatgtg
      661 ctgcttgccg tggtatggat gcaaggggaa tttgatttta acggaacgcc agcgaatcat
      721 gcagcccgtt ttacagaagt ggtggatcaa taccgtgcgg accttgcaga tatggcgggg
      781 cagtgtgtgg gtggctcagc tgacagtgtt ccctggattt gtggagacac aacatatttc
      841 tggaagcaga agaacgaaac ggcgtaccag acggtgtatg gtagttacaa aaataaaacg
      901 gaaaagaata tccatttcgt gccgttcatg accgatgaga acggggtgaa tgtgccgacg
      961 aacaaaccgg aagaagaccc ggatattccg gatatcgggt attacggttc aaaatggcga
     1021 acggacagaa gcacctggac atctcaggac agggccagtc atttcagttc atgggctcgc
     1081 cgtgggatta tttccgaccg tctggcaacg gcgattctga gctgcgcggg taagtcttct
     1141 gcgtttgtta atggtactgc cggggtggtt gttccagaca gaccggttac cacctcagag
     1201 tctgtaattt tttacgatgc caaaaaagct tcagacaatc agctgaaacc ttatggctgg
     1261 gacggtatgg atggcagacg cacactggtt gatgacagcg gcaataaagc tctgcgaatt
     1321 gagaaaaata acagcgcgaa atcctggtca atgtactgtg atattgctgc agacaaggca
     1381 aaacttttac tggaaaaagg cggggaaatt gctgtccggt ttaaaatccc cgaaaacgtc
     1441 aatcttgaga caaccagaaa caagtatgcc tttggtttgt actggcgaat agcggaatgg
     1501 ccgggtgagg gtggtgaagg ctatctgagt tctttctttg tccagacaga taaagccagt
     1561 attgatgttg cataccatca tacagttaat caacaaaaag aacttggcac gtttggcgca
     1621 ttcgaccatg actggcatac gcttgcattt aaatttaagg gcagtaacag cattaatgtt
     1681 actccggtgc ttgatggtgt ggatggacag gcgtttgacc tggtgaaatg ggccaatact
     1741 gctaatggac tcaacaggtt tgtcattacg gatattacag gtagtgcaga aacctaccct
     1801 gtacttattg atacggtgga agttaaagca aacaaagctg gagcagccgc ataattgcta
     1861 aaaaaagccg ccagcggcag gaatggaagc tggcggaggt aatcccaatg gagaatgtaa
     1921 agaaaagatg ctttcgtaca ttggtttttt aaatgaaaac agttctcatt gtcaaccata
     1981 acggtaagaa attatgatat ttattcatca ggtgatgctg tacttctgta cggcggtctg
     2041 tgtgctgtat cttctttcgg gtgggtacag ggctgtgcgc gatttctggt gcaggcagat
     2101 tgataaaagg gccgctgaga aaatcagcgc cagtcagtca gccggaagca aacccgaaga
     2161 gcccgttact ccttaacaac ccctttcagc gagaaaatcc catgtcagaa atcacatccc
     2221 tggtcactgc agaggcagtg aaggaagtcc tgcgctctga agaagtccgg agcgcactga
     2281 aacagaaact tcgccagaat cttgaggcgc gtcttgatgc agaagttgat gccattctgg
     2341 atgagctgct tggtgtacag gcagagccac cgactgaagc gggagatacc accgcagaga
     2401 gcggtgaagt tcagcctgaa tcaccggtcg ccgatgcgac tgaacctcaa cccgaatcgg
     2461 tcatgatgct gtaacgagga gtcagggcca tcagtaaacg gctgctggcc tttttcatgt
     2521 tgtgagcttc cggattgcgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt
     2581 gtgtcataca ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg
     2641 gtttccccgt ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tgggctgctg
     2701 acatatctga cgaacctgta tttcaaaatt aaagaagacc ggcgtaaggc ggcgcgggga
     2761 gagtaagctg atgagcagga aattccgcta tggtttatca gcggtcgttc tggcgatgat
     2821 tgctgcaggt gcttctgcgc ctgaaatcct cgaccagttt cttgacgaaa aagaaggtaa
     2881 tcgcaccaca gcataccgtg atggtgcggg tatctggacc atctgccgtg gtgccacccg
     2941 ggtggatggt aagcccgtcg ttcccggcat gaagctgtcg aaggaaaaat gcgcacaggt
     3001 taatgccatt gagcgtgata aggcgctggc atgggtggag aaaaacatca aagtgccact
     3061 gaccgaaccc cagaaagcgg gtattgcgtc attctgcccg tataacattg gccccggtaa
     3121 gtgtctcccg tcgacgtttt ataagcggct taatgccggt gatcgtaaag gtgcctgtga
     3181 gtcgattcgc tggtggatta aggacggtgg gcgtgattgc cgcacacgtt caaataactg
     3241 ctacggacag gttattcgtc gtgaccagga aagcgcatta gcttgttggg ggatagatca
     3301 gtgagaagag taaccgcgat tatttacgct ctggttatct gcatcatcgt ctgtctgtta
     3361 tgggctgtta atcattaccg cgataacgcc atcacctaca aagcgcagcg cgataaaaaa
     3421 gccagagagc tggagctggc aaacgcaacc attactgaca tgcagcagcg ccagcgtgat
     3481 gttgctgcgc ttgatgccag atactcaagg gaattagccg atgcgagagc tgaaaatgaa
     3541 actctgcgcg ctgatgttgc cgctggtcgt aagcgcctgc ggatcaacgc cacctgtcca
     3601 ggctccgtgc gtgaagcccc caccacctcc ggcgtggata atgcaaccgg cccccgactg
     3661 gcagacacag ttgaacggga ttatttcacc ctcagagagc ggttgatgat gatgcagaag
     3721 cagctggaag gggcacagga atatatccgc actcagtgcc tgaaataagt tttgctgatg
     3781 cgccgtatcg tcgccgtatt cccgtattaa cagcgactgc agccctgacg ggtacctcct
     3841 gcgtgagtgt gcggggataa tcaaaaacga tacacaccgg gtttccctca tttttcagga
     3901 gacgtgagca tttacccgcg aagccgcctg tccggtgcgg tggtggaaga aaccggataa
     3961 aacaacctca ttgtacaaat atcgatcaag tatggcgctg ctgtgtgaat tctgaaaaat
     4021 cacagcggtc attatgcgtc agtttttaac agaggacgtc agaaagtgac atggcaaagc
     4081 tggactggaa aaagctggag caggcattcc gacgcgaaca tgccgaaacg ggaataacat
     4141 tactggactg gtgccggaag aaaaagatta attacaacac cgccagaacc cgtataaaaa
     4201 tgggcaaaat cgatcatgaa attgatcata aaaccgatca tgaaatcgat catgacatct
     4261 cagatgaaga acccagcaat gacgcgtgtt ccgacgatga aaaatgcgca aaaaactctg
     4321 aaaaaaactg cgcaaattcg gcagaaacga aacggattcg tggttcccga cttttacctc
     4381 cttcaaacgc tttttctcag cgaaacaccc acgccgtaag acaccgtgga tatgcgaagt
     4441 atcttgaggc agataacctc atggatgatg cgtccgacat ggtgctgttc gatgaactgg
     4501 tgttcacccg ggcccgcgca ctttcagtaa ctaaggcact taaagggatg ttcgccgacc
     4561 tggaagaggc aactgacgtg gaaacccgcg ttgctcttta cgacaaaata ctcaaagctg
     4621 aacaggccct tgaccggaat attgcccgta tcgagtcaat tgaacgctca ttgctgacgc
     4681 tggacgtcct ggctgagaca gcaccaaaac ttcgtgctga ccgggaaaga atcaacgccg
     4741 ccagagataa actcagagcg gaaaccgata ttctgaccag ccagcgccgg ggggttatta
     4801 cgccagtcag tgacatcgtg tcatcgctgc atgaaatgag taattcgggg agactggatg
     4861 acattccgga agaataaaac gcgatgtgat gaacctgcag aaatgactga gaccgaacaa
     4921 cgtctgttca ttatgacaaa actgagcaat ccctggtggc gcctcaatca tctctacaaa
     4981 atacagaacg aaaaaggtga actggtcacc ttcagaatgc gaccggcgca acgtcagttg
     5041 tttcgtaaca tgcataataa aaacattatc ctgaaagcgc gccagctggg attttccaca
     5101 gccattgata tttatcttct cgaccaggca ttattcattc cgcatctcaa atgcgggatc
     5161 gtcgctcagg ataaacaggc tgccagtgaa attttccgca caaaaattgc tgtaccgttt
     5221 gatcatctcc ctgactggct gagagcctca ttcaccatcg ttgaacgtcg tagcggtgcc
     5281 agcggtggct atatcctgtt tggtcacggc tcgagtattc aggtggcaac ctcatttcgc
     5341 tcaggtacgg tgcagcgcct gcatatctca gagcacggca aaatttgcgc gaaatatccg
     5401 gctaaggcga aagagctgcg aaccggtacg cttaatgccg tctctgatga atgcattatt
     5461 tttgatgaat ccacggctga aggcgtgggt ggtgattttt acgagatgag taaccgtgca
     5521 caggagagca ctgcatcagg cttattgctg acggcacagg attataaatt ccatttttac
     5581 gcatggtggc aggatcctaa atacagcgcc agagtgcctg aaagcgggct gaagctgtca
     5641 cgggaaaaaa tgacgtattt ttctgcggtt gagaaggcaa tgaacatcac gcttaccgat
     5701 gagcagaagc agtggtacat cagtaaggaa actgaacagc gtgaggaaat gaagcaggag
     5761 tttccctcaa cgccacagga ggcgtttctg acgtccggac gacgtgtgtt cagtgccgaa
     5821 agtacattgc aggcagaatc attctgttcg ccaccgatga ttgtttatga cattgaacct
     5881 gttacaggag cgaagactaa agctcagtct ctgcgtgaag gaaataaaaa cgagttgcag
     5941 cggacgctga tgaattatct gctggtatgg gaactgccgg atccggatga agagtatgtt
     6001 tgtggggcag atactgccga agggctggag cacggagacc gctcatcgct ggatgttgtc
     6061 aaacgcagta atggcgagca ggtggctcac tggttcgggc atctcgatgc tgaacttttt
     6121 gctcatctca tttcgcaggt ctgtcgtatg tataacaacg cgtttgtggg gccggagcgt
     6181 aataatcacg gacatgcagt tatcctgaaa ctccgggaac tctatccgac acgttatatc
     6241 tacaacgaac agcatcttga ccaggcatat gacgacgata cgccccgcct tggctggctg
     6301 acaacccgtc agagcaaacc tgttctgacc gaaggaatga aaacgctcct gaataatgga
     6361 atatcaggga tccgctggtc aggcacatta tcggaaatga acacctacgt ttatgacgcg
     6421 aaaggctcca tgaatgcaca ggaaggctgc tttgatgatc agctcatgag ctacatgatt
     6481 gcccaggaga tgcgcgccag aatgccggtg agggtaaaac agaaaacgga taaacgcaga
     6541 accacacact ggatggcaca ctgatgaaaa atgaaattaa caccacagcg atgaaaaacg
     6601 atcatggatc cacgccgcgt ttttctcagc gtcagttact gtctctctgt tctgatattg
     6661 acagtcagcc tctctggcgt gatgccgcaa acaaggcctg tgcgtattat gatggcgacc
     6721 agctggcacc ggaagttatc caggtactga aagatcgcgg tcagcccatg accatccata
     6781 acctcattgc ccccacggta gatggtgtac tgggaatgga ggcaaaaaca agaacggacc
     6841 tgatagtgat gtcagacgat ccgaacgatg aaacagagaa actggcagag gccattaatg
     6901 cggagtttgc tgatgcgtgc cgtcttggca acatgaataa ggcccgctcc gatgcctatg
     6961 cggaacaaat caaggcgggg ctcagttggg tggaggtcag gcgcaacagc gatccgttcg
     7021 gacctgaatt cagggtatcc acagtcagca gaaatgaggt tttctgggac tggctcagcc
     7081 gggaatccga cctgagtgac tgccggtggc tgatgcgtcg ccgctggatg gataccgatg
     7141 aggcaaaagc cacgttcccg ggaatggctc aggttatcga ttatgctatt gatgactggc
     7201 gtggttttgt cgataccacg attactgaag gccagcccag tccgttgatg agtgcatggg
     7261 aagagtatca gtcatgggat cgcagggaga atgaatggct tcagcgtgaa cgccgtcgtg
     7321 tgctgcttca ggtggtttat taccgtacat tcgaacgtct tccggtgatt gaactcagta
     7381 atggacgggt ggtggctttt gataaaaata atctgatgca ggcggtggct gtggcatccg
     7441 ggcgggttca ggtgaaagtc gggcgggtaa gtcgtattcg tgaagcctgg tttgtgggcc
     7501 cacactttat tgtggatcgc ccctgtagtg ctccgcaggg gatgtttccg ctggttcctt
     7561 tctggggata ccgaaaggat aaaaccgggg agccatacgg gttaatttcc cgcgccattc
     7621 cggcacagga tgaggtgaat tttcgtcgta tcaagctgac ctggctgctt caggccaaac
     7681 gcgtgattat ggacgaggat gccacccagt tgtcagacag cgacctgatg gagcagatcg
     7741 aacgtccgga tggcattatt aaactgaatc cggcccgaaa aaatcagaaa agtgtcgcag
     7801 atgtttttcg ggttgagcag gattttcagg ttgccagcca gcagtttcag gtcatgcagg
     7861 aatcggaaaa acttatccag gataccatgg gagtgtattc cgcatttctc gggcaggatt
     7921 caggtgcgac gtcaggcgtg gctatcagta acctggtgga gcagggggcc acaactcttg
     7981 cggaaatcaa cgataactac cagtttgcct gccagcaggt gggaagactg ttgctggctt
     8041 atcttctcga tgacctgaaa aaacgccgta atcatgcagt ggtgattaat cgcgatgatc
     8101 gccagcgtcg ccagaccatt gtcctcaatg ctgaaggtga taatggtgaa ctgaccaatg
     8161 atatttcaag gttaaataca catattgcgc tggcgcctgt tcagcagaca ccggcgttta
     8221 aggcacagct tgcacagaga atgtcagagg ttattcaggg gctgccgcct caggtgcagg
     8281 ctgttgtgct cgacctgtgg gttaatcttc tggatgtgcc gcagaaacag gagtttgttg
     8341 agcgtattcg tgctgcgctg gggacgccaa aatcaccgga tgaaatgacg ccggaagaac
     8401 aggaagtagc ggcacaacaa caggcacttc agcaacaaca ggcagaactc cagatgcgcg
     8461 agatggctgg cagagtggca aaactggaag ctgacgccgc cagggcacat gcagctgcac
     8521 aacgggataa tgccagtgca cagcgggaag tcgccctgac acaggggcag cgttatgtgg
     8581 atgcgcttaa ccaggcacat acggcagaaa tcattaccgg cgtacagaat atggaacagg
     8641 agcaggacgt tcttcagcaa cagatgctgt atacgttaca acagcggatg aatgaaatgt
     8701 cgctctgaaa actctggctt caactgaacc ccgtcatcgt acggggtttt ttgtttccgg
     8761 aggtaagcgt tccgggagcg gtgcgcttat tcgcgggggc agcgataagc cttatttact
     8821 caaccattcg gatctgtccg ataaacagac catgcggagt tatttatgga ttttgaattt
     8881 acgggtgaag aaaccccgga acaactggaa aaaatgctgg aaggacttgg ggatgtggat
     8941 attgacagtc acgcacagga cgtcgtgacg gaagatacca cggaaaaaca tgcggatgag
     9001 gaagcacaga ctcagacggg cgataacaat gtggcaccga cgccggatgc cagtgtggag
     9061 cagacgcagg acgtgaagga gccggaagcg aagggggtgc tcacccgcga cggtaaacac
     9121 gtcattccct atgaagtcct tgaggctgaa cgttccggta agcaacgggc cgaacaggaa
     9181 gccgcacttc ttcgtgggca gatagctgaa gaaaaacgca gggtggaact gctgacgtct
     9241 cagatccacc aggccggtat gaagcccaca ccgttaccgg aaaacgaaaa aatttctgat
     9301 gagcagattg cccgtatcag ggagatgtat ccggaaattg gtgacgcggt ggcttcgctc
     9361 atccgtaaaa ataactatct ccagtcccgt gttcagcaat caacacagca ggcagaaggt
     9421 aatggtggtg aggatttatc accggttctt gatgcgatga atgccgtgcc ggtgctgaaa
     9481 acgtggcagg agtccgatcc ggatcgcttc tcggttgctg tatccatcga cgggaagctc
     9541 cagaatgacc ccgcatggaa agacaaaacg ctcactgaac gtttcgctga agtggcccgt
     9601 cgtacgcagg ttgctttcgg tgaagtcagt gagtcgtctg ctgacaacca ggcagacaaa
     9661 acggatatcc ggaaaacggc ggaagaaaaa gtgaagaccg ctgaacagga gcaggcagta
     9721 cctgcttccc cgtcagattt aggcaccacg gcctccgtcg gaaccggtga taattttgaa
     9781 cggttacttg gtgcttctca ttcagaggct gaggcgatta tgcgtggtat gacgaatgct
     9841 gaaatagacg cacttctgga gaagctcggg taacttactg aaggagtact gaagtaatga
     9901 cgactgtaac atcagcccag gcgaataagc tgtatcaggt ggcgcttttt actgctgcca
     9961 accgcaaccg ctcgatggtt aatatcctta ctgaacagca ggaagcgcca aaagcggttt
    10021 cgccggacaa gaaaagcacg aagcagacca gcgcaggtgc gccggttgtc cgtatcacag
    10081 accttaacaa acaggccggt gatgaagtga ccttcagcat catgcacaaa ctctcaaaac
    10141 gtccgacgat gggagatgag cgtgttgaag gtcgtggtga ggatctcagc catgctgact
    10201 tctccctgaa aatcaatcag ggacgtcacc tggtggatgc aggcggacgt atgagtcagc
    10261 agcgcacgaa gtttaacctg gcatcctctg ccagaacgct tctggggacg tactttaatg
    10321 acctgcagga ccagtgtgcg atagtgcatc ttgccggagc acgtggtgat tttgttgctg
    10381 acgacactat tctgccgaca gcggagcacc ctgaattcaa aaaaatcatg atcaacgatg
    10441 tactgcctcc gacacatgac cgtcactttt ttggcggtga tgcgacaagc tttgagcaga
    10501 ttgaagcggc agatattttt tctattggcc tggtggacaa tctctccctg ttcattgacg
    10561 aaatggcgca tccgttacag ccggttcgtc tgtccggtga tgaacttcac ggagaagatc
    10621 catattacgt cctgtacgtc acgccgcgtc agtggaatga ctggtacacc tcgacgtccg
    10681 gtaaggactg gaaccagatg atggttcgtg ccgtgaaccg tgcaaaaggt tttaatcatc
    10741 cgctgttcaa aggtgaatgt gcgatgtggc gcaatatcct ggttcgtaag tatgcgggta
    10801 tgccgatccg tttctatcag gggtcaaagg ttctggtatc agagaataac ctgacggcaa
    10861 ccacgaaaga ggtcgctgct gcaaccaata ttgaccgcgc catgttactg ggggctcagg
    10921 cgctggcaaa tgcttacggt cagaaggcgg gcggtcactt caacatggtt gagaagaaaa
    10981 cggatatgga taaccgtact gagatagcaa tcagctggat caacggtctg aaaaaaatcc
    11041 gtttccccga gaagagcggc aagatgcagg atcacggcgt gattgccgtt gatacagcag
    11101 tgaagctctg attttttcct ttccccatgc cgggttttcg cccggctttt tcaggagtca
    11161 ttaattatgg caaagactat ccttgccccg tcactgagtg aacgggtcta tacgggtacg
    11221 cacggtaatg agtcggtggc agaaggcgta tttacggtga atgctgcgga agcggacagt
    11281 gttattcatc ttctctcact gccagtgggc atccgtatca actcactcca gctggtttca
    11341 acgggtggtc tgggtactgc aaccgtcagc attaagtccg gtgagcatgc tctcatcgat
    11401 aacagcgaag ctgtttctgc aaaatttgcc agatatgtgc cagtggagcc gtacaccaca
    11461 cagcgtgacg gggagctggt tactgtcacc attaagactg ccgctgcaac cggcaccctg
    11521 aatgttctgc tgcgttatac cgtggtggga tactgattaa aaccttccgg cccgcgtcat
    11581 gcgggctttt tatccgggga attatatgag tgagaaaatt gccgttgtct atatcggccc
    11641 aaaacccgtg aaaaaggaca ccattaccgg aagccgcaca ttgttcccac gtcttgagcc
    11701 ggtgcatgtt gacagtgcga tggcctggca actgctgggg tttccggatg tctgggttcg
    11761 tcatgaagag cttgatgatg ttctgaaaaa gcaacaacag aatgagcagt tgcggcaggc
    11821 acagcaggcg caggaaagcg tgcttgctgc gcgggcagaa gcggagaaca gttttgttgt
    11881 ttctgttaac gggcaggagg tggatttaag taagctcacc tcagcacggc tggcgacgct
    11941 gtgtgaggca gaagagctgg atattcacaa agacccgaaa gaaacggctg aggcattccg
    12001 tatccgggtg cgtgaggcat ttcgccgtcg tgttgcggag actgaacagc atggcggaac
    12061 tgagtgattt tttaccgtat gtccgtcgtc atatcagcgg tccactgaac attatgatga
    12121 cggatgctct gtcaatggct gccgtggcat tcagccgcca gtcgttggtg tgccgtcggg
    12181 aggttactgt tgtaccggtg gcaggaaaag aaatcgtgct tccgtatgac aaagatgatg
    12241 aggagtgcgt tcatatcatc cgtatctctg acgataatca tgagcttttt gtcggtcggg
    12301 atgtggatat cagctccgga cgctccctgc gatttgcctg ttctcccggt gaggtgagcg
    12361 tgctttatgc cgtcgctccg aaagccggac gcagccagat accggatgaa ctcctcacat
    12421 ggcctgaaga agtggctgcg ggggcacttg agcggttgtt catgcagact ggtgtttcat
    12481 ggtcagatcc gttacgcgca cagtattttt ctgtgcagtt ttctgagggg atccgtcggg
    12541 catatcgtca tacactggcg acaagcccgt actcttcata ccgcaaccct gtacgcaggc
    12601 agaggtttta ctgatgacga cgattacaga aatcatcgga cgtgtgaata cacaactggt
    12661 tgacccgatg atggttcgct ggcccctgca ggaattgtgc gattattaca atgatgctgt
    12721 gagggcagtg attctggcga gaccggatgc tggcgcaagc ctggaaacaa tcagttgtgt
    12781 tcctggcgcc cgtcaggttt tgcccgatgg tgtaatacaa cttcttgacg tgatatgcct
    12841 cagtgatggt agtgcagtca gaccattatc ccgggaggtg ctggatgcgc agtatcctga
    12901 gtggcccaca atgaagggca ttcctgaatg ttttatcagc aacgacctgt ccccgcgcgt
    12961 attctggctg tttcctgtcc ctgacaaaga gataagtatt gatgcagtgg taagccggat
    13021 accggaggca gtgtatgttc tgacgcagga cgatgatacg ccagttccac tggaagaggc
    13081 ttatgttaac ccactggtgg actggatgtt gtttcgtgct ttcagtaagg atgctgccgg
    13141 tggtgcagaa tcggggctgg ctgcgcagca ttatcagagt tttgttgagc aacttgggat
    13201 caaacagggg gcagacagtg cattgtctgc ccgtaaaaaa gtgtttaacg gaggtggagt
    13261 gtgagtgttg ttgtttcggg gacgctgaaa tctcctgatg gtgaggcgat atcaggagca
    13321 aatattaccc tgacggcgct gacagtttca ccggatgcgc tcagcggcac cagtgcgtcg
    13381 gcagtgaccc gtgaaggtgg atattacgga atgacgatgg atccggggga gtatgcggtt
    13441 tcggtgacgg tgaaagggaa gactgctgtc tacggacgtg tgcgtattga ggggaccgaa
    13501 agtacggtga cgctcaatat gctgttacgc cgcagtcttg ttgaggtgag catacccgga
    13561 gagctgctga cagatttccg gcagatacag aataatgtgg ctgatgacct tgctactatt
    13621 cgtcgcctga atgaagacac ggcgacaaaa aacactcagg ccacacagtc aaaagaaagt
    13681 gcagcagcca gtgcgaagag tgcatctgac agtgcaaaga cggcaaccag cagggcggct
    13741 gaagccggac aaaaagccag tgaagctgca gaagcagcga cccgtgcaat cacagcagcg
    13801 gggaatgcag aggaaagctc gacccgtgcc ggagagtctg aaaaagccgc cggagctgat
    13861 gcagaaaaag ccagactgca tgctgaaaag gccaggctgg cgcaggagag cgccggagag
    13921 atccttaagc gggcagaggc tgccactgtc agtgctgaag aggccagacg tatggctgag
    13981 aatgcacggg ggccccgggg gcctcaggga gaaactggtc cgaaggggga tgtcggtcct
    14041 aaaggcgaaa caggtccagt gggccctcaa gggcccgcag ggccgaaagg tgagcgtggt
    14101 gacgttggtg ctcagggggc agtagggccc gctggtccgc ggggtgagaa gggcgaacag
    14161 ggggagcgag gaccgcaggg aatacc
//
LOCUS       DAZGTJ010000113        14064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000113 DAZGTJ010000000
VERSION     DAZGTJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.113"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            204..1313
                     /gene="livK"
                     /locus_tag="ACHK8E_004349"
     CDS             204..1313
                     /gene="livK"
                     /locus_tag="ACHK8E_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP0346534.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1361..2287
                     /gene="livH"
                     /locus_tag="ACHK8E_004350"
     CDS             1361..2287
                     /gene="livH"
                     /locus_tag="ACHK8E_004350"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP0346535.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2284..3561
                     /gene="livM"
                     /locus_tag="ACHK8E_004351"
     CDS             2284..3561
                     /gene="livM"
                     /locus_tag="ACHK8E_004351"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP0346536.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3558..4325
                     /gene="livG"
                     /locus_tag="ACHK8E_004352"
     CDS             3558..4325
                     /gene="livG"
                     /locus_tag="ACHK8E_004352"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP0346537.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4327..5040
                     /gene="livF"
                     /locus_tag="ACHK8E_004353"
     CDS             4327..5040
                     /gene="livF"
                     /locus_tag="ACHK8E_004353"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP0346538.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5128..5349
                     /locus_tag="ACHK8E_004354"
     CDS             5128..5349
                     /locus_tag="ACHK8E_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0346539.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            5346..5723
                     /locus_tag="ACHK8E_004355"
     CDS             5346..5723
                     /locus_tag="ACHK8E_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP0346540.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            5932..7248
                     /gene="ugpB"
                     /locus_tag="ACHK8E_004356"
     CDS             5932..7248
                     /gene="ugpB"
                     /locus_tag="ACHK8E_004356"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP0346541.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            7424..8311
                     /gene="ugpA"
                     /locus_tag="ACHK8E_004357"
     CDS             7424..8311
                     /gene="ugpA"
                     /locus_tag="ACHK8E_004357"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP0346542.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8308..9153
                     /gene="ugpE"
                     /locus_tag="ACHK8E_004358"
     CDS             8308..9153
                     /gene="ugpE"
                     /locus_tag="ACHK8E_004358"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP0346543.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9155..10225
                     /gene="ugpC"
                     /locus_tag="ACHK8E_004359"
     CDS             9155..10225
                     /gene="ugpC"
                     /locus_tag="ACHK8E_004359"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP0346544.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10222..10965
                     /gene="ugpQ"
                     /locus_tag="ACHK8E_004360"
     CDS             10222..10965
                     /gene="ugpQ"
                     /locus_tag="ACHK8E_004360"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0346545.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10952..11392)
                     /gene="yhhA"
                     /locus_tag="ACHK8E_004361"
     CDS             complement(10952..11392)
                     /gene="yhhA"
                     /locus_tag="ACHK8E_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP0346546.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11512..13257
                     /gene="ggt"
                     /locus_tag="ACHK8E_004362"
     CDS             11512..13257
                     /gene="ggt"
                     /locus_tag="ACHK8E_004362"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP0346547.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(13295..13579)
                     /gene="yrhB"
                     /locus_tag="ACHK8E_004363"
     CDS             complement(13295..13579)
                     /gene="yrhB"
                     /locus_tag="ACHK8E_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP0346548.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(13821..13934)
                     /locus_tag="ACHK8E_004364"
                     /pseudo
     CDS             complement(13821..13934)
                     /locus_tag="ACHK8E_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3184 a         3483 c         3970 g         3427 t
ORIGIN      
        1 ttctgtttat ctatcaataa attcagaata ttatctgttc ttaatcgact gaaaaatagg
       61 gattttaatc gctatcatca caaaatactg tgctaaaccc ttaatcagac aggcaaaaac
      121 agtgcagtat aaaaaaagaa cagtctgatt tgttaacaca taagaacaaa gcaacacaac
      181 atcacgaatg gggatttttg actatgaaac ggaatgcgaa aactatcatc gcagggatga
      241 ttgcactggc aatttcacac accgctatgg ctgacgatat taaagtcgcc gttgtcggcg
      301 cgatgtccgg cccgattgcc cagtggggcg atatggaatt taacggcgcg cgtcaggcga
      361 ttaaagacat taatgccaaa gggggaatta aaggcgataa gctggttggc gtggaatatg
      421 acgacgcctg cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt aacgacggca
      481 ttaaatacgt tattgggcac ctgtgttcct cctctaccca acctgcatca gatatctacg
      541 aagacgaagg tattctgatg atttcgccgg gagcgaccaa tccggagctg acccaacgcg
      601 gttatcaata catcatgcgt actgccggtc tggactcttc ccaggggcca acggcggcaa
      661 aatacattct tgagacggtg aagccccagc gcatcgccat cattcacgac aaacaacagt
      721 atggcgaagg gctggcgcgt tcggtgcagg acggattgaa agcagccaat gccaacgtcg
      781 tcttcttcga cggcattacc gctggtgaga aagatttctc cgcgctgatc gcccgcctga
      841 aaaaagaaaa catcgacttc gtttactacg gcggttacta cccggaaatg gggcagatgc
      901 tgcgccaggc ccgttccgtt ggcctgaaaa ctcagtttat ggggccggaa ggggtgggta
      961 atgcgtcgct gtcgaacatt gctggtgatg ccgccgaagg tatgttggtc actatgccaa
     1021 aacgctatga ccaggacccg gcaaacaaag gcatcgttga tgcgctgaaa gcagacaaga
     1081 aagatccgtc cggaccttat gtctggatca cctacgcggc ggtgcaatct ctggcgactg
     1141 cccttgagcg taccggcagc gatgagccgc tggcgctggt gaaagattta aaagctaacg
     1201 gtgcaaacac cgtgattggg ccgctgaact gggatgaaaa aggcgatctt aagggatttg
     1261 attttggtgt cttccagtgg cacgccgacg gttcatcgac ggcagccaag tgatcatccc
     1321 accgcccgtg aaatgcgggc ggttttagaa aggttacctt atgtctgagc agtttttgta
     1381 tttcttgcag cagatgttta acggcgtcac gctgggcagt acctacgcgc tgatagccat
     1441 cggctacacc atggtttacg gcattatcgg catgatcaac ttcgcccacg gcgaggttta
     1501 tatgattggt agctatgtct cctttatgat catcgccgca ctgatgatga tgggcattga
     1561 taccggctgg ctgctggtgg cggcgggatt cgtcggcgca atcgtcattg ccagcgccta
     1621 cggctggagt atcgaacggg tagcctatcg tccggtgcgt aactctaagc gcctgattgc
     1681 gcttatttcc gcaatcggca tgtctatctt cctgcaaaac tacgtcagcc tgaccgaagg
     1741 ttcgcgcgac gtggcgctgc ccagcctgtt taacggtcag tgggtggtgg gccatagcga
     1801 aaacttctct gcctctatta ccaccatgca ggcggtgata tggattgtta ctttcctcgc
     1861 catgctggcg ctgacgattt tcattcgcta ttcccgtatg gggcgcgcct gccgtgcctg
     1921 cgcggaagat ctgaaaatgg cgagcctgct tggcatcaac accgaccggg tgattgcgct
     1981 gacctttgtg attggcgcgg cgatggcggc agtggcgggc gtgctgctcg gtcagttcta
     2041 cggcgtaatt aacccttaca tcggctttat ggccgggatg aaagccttta ccgctgcggt
     2101 gctcggtggg attggcagca ttccgggggc gatgattggc gggctgatcc tggggattgc
     2161 ggaggcgctc tcttctgcct atctgagtac ggaatataaa gatgtggtct cattcgccct
     2221 gctgatcctg gtgctgctgg tgatgccgac cggtattctg ggtcgcccgg aggtagagaa
     2281 agtatgaaac cgatgcatat tgcaatggcg ctgctctctg ccgctatgtt ctttgtgctg
     2341 gcgggcgtct ttatgggcgt gcaactggag ctggatggca ccaaactggt ggtcgacacg
     2401 gcttcagaga tccgttggca gtgggtgttt atcggcacgg cggtggtctt tttcttccag
     2461 cttgtgcggc ctgctttcca gaaagggcta aaaagcgttt ccgggccgaa gtttattctg
     2521 cccgcgattg atggctccac ggtgaagcag aaactgttcc tcgtggcgct gctggtgctt
     2581 gcagtggcgt ggccgtttat ggtgtcgcgt gggacggtgg atatcgccac tctgaccatg
     2641 atctacatta ttctcggtct cgggctgaac gtggttgttg gtctttctgg tctgctggtg
     2701 ctgggatacg gcagctttta cgccatcggc gcttacactt ttgcgctgct caatcactat
     2761 tacggtctgg gcttctggac ctgtctgccg attgccgggt tgatggcggc ggcggcgggc
     2821 ttcctgctcg gttttccggt gctgcgtctg cgcggtgact atctggcgat cgttaccctc
     2881 gggttcggcg aaattgtgcg catattgctg ctcaataaca ccgaaattac cggcggaccg
     2941 aacggtatca gtcagattcc gaaaccgacg ctcttcggac tcgagttcag ccgtaccgcc
     3001 cgcgaaggcg gctgggacac gttcagtaat ttctttggcc tgaaatacga tccctccgat
     3061 cgcgtcatct tcctctacct ggtggcgttg ctgctggtgg tgttaagcct gtttgtcatt
     3121 aaccgcctgc tgcggatgcc gctgggccgt gcgtgggaag cgttgcgtga agacgagatc
     3181 gcctgccgtt cgctgggctt aagcccgcgt cgtatcaagc tgactgcctt taccatcagc
     3241 gccgcgtttg ctggttttgc cggaacgctg tttgctgcgc gtcagggctt tgtcagcccg
     3301 gaatccttca cttttgccga atcggcgttt gtgctggcga tagtggtgct cggtggtatg
     3361 ggttcgcaat ttgcggtgat tctggcggca attttgctgg tggtgtcgcg agagttgatg
     3421 cgtgatttca acgaatacag catgttaatg ctcggtggtt tgatggtgct gatgatgatc
     3481 tggcgtccgc agggcttgct gcccatgacg cgcccgcaac tcaagctgaa aaacggcgca
     3541 gcgaaaggag agcaggcatg agtcagccat tattatctgt taacggcctg atgatgcgct
     3601 tcggcggcct gctggcggtg aataacgtca atcttgaact gtacccgcag gagatcgtct
     3661 cgttaatcgg ccctaacggt gccggaaaaa ccacggtttt taactgcctg accggtttct
     3721 acaaacccac cggtggcact attttactgc gcgatcagca tctggaaggt ttgcccggcc
     3781 agcaaatcgc ccgcatgggc gtggtgcgca ccttccagca tgtgcgtctg tttcgtgaaa
     3841 tgacggtgat tgaaaacttg ctggtggcgc agcatcagca actgaaaacc gggctgttct
     3901 ccggcctgtt gaaaacgcct tctttccgcc gcgcccagag cgaagcgctc gaccgtgccg
     3961 caacctggct tgagcgcatt ggtttgctcg aacacgctaa ccgtcaggcg agtaacctgg
     4021 cctacggtga ccagcgccgt ctggagatcg cccgctgcat ggtaacgcag ccggagattt
     4081 taatgctcga cgaaccggca gcaggtctta acccgaaaga gacgaaagag ctggatgagc
     4141 tgattgccga actgcgtaac catcacaaca ccactatctt gttgattgaa cacgatatga
     4201 agctggtgat ggggatttca gacagaattt acgtggtcaa tcaggggacg ccgctggcaa
     4261 acggtacgcc ggagcagatc cgtaataacc cggacgtgat ccgtgcctat ttaggtgagg
     4321 cataagatgg aaaaagtcat gttgtccttt gacaaagtta gcgcccacta cggcaaaatc
     4381 caggcgctgc atgaggtgag cctgcatatc aatcagggcg aaattgtcac gctgattggc
     4441 gcgaacgggg cggggaaaac caccttgctc ggcacgttat gcggcgaccc acgcgccacc
     4501 agcgggcgaa ttgtgtttga tgataaagac attaccgact ggcagacggc gaaaatcatg
     4561 cgcgaagcgg tggcgattgt cccggaagga cgtcgcgtct tctcacgcat gacggtggaa
     4621 gagaatctgg cgatgggcgg cttttttgct gaacgcgacc agttccagga gcgcataaag
     4681 tgggtgtatg aactgtttcc acgtctgcat gaacgccgtg ttcagcgggc aggcaccatg
     4741 tccggcggtg aacagcagat gctggcgatt ggtcgcgctt tgatgagtaa cccacgtttg
     4801 ttattgcttg atgaaccctc gctcggactg gcaccgatta tcatccagca aattttcgac
     4861 accatcgagc aactgcgcga gcaggggatg accatcttcc tcgtcgagca gaacgccaac
     4921 caggcgctga agctcgctga tcgcggctac gtgctggaaa acggtcatgt ggtgctctcc
     4981 gatactggcg atgcgctgct ggcgaacgaa gcggtgagaa gtgcgtattt aggcgggtaa
     5041 taatattttg agtaaaaagg agtcgaatga ctcctttttt gtacttgtta gttgtacaat
     5101 ttaattgtac ttaatatgga ggatatcatg cataccatga actacagcga agcgcggcag
     5161 aaccttgcat tagttctgga aacagcggcg acaggtactc cggtaacgat cacgaggcgt
     5221 gggcataagg cagctgtgat tatcagcgca gaagagtttg aacgttacca gaaggctaaa
     5281 ctggacgcag aatttgacgc catcatggct attcatggca gcgaaattaa ggagttagcg
     5341 gataaatgac cattcagttt atctcagcgg aggagataat ttatttccac gataaattgc
     5401 ttagcgttac accaggtgta acaggaatgt ccgatccagg gcgagcagaa gcgcttttgt
     5461 accgggtact gaataagtat gagtatgaag gcgtttctga tctttggctg ctggcggcga
     5521 tgcatctatt ggcaatctcg cgcggtcata tctttaatga tggtaataag cgcaccgcac
     5581 tgtttatcac gctattattc cttaaacgta acggcatcat acttgctgca aacccagagt
     5641 ttgtagaatt gacggttgat gtggcggcgg gaaaagtatc gttagaagat atcgttaccc
     5701 gcttgcgtca acgcggtacc tgacaaggtt cttagccatt ctgcttttta tccgtgcccc
     5761 atctcccatt ttccctcacc cacgccgtca ccgccttgtc atctttctga caccttacta
     5821 tcttacaaat gtaacaaaaa agttattttt ctgtaattcg agcatgtcat gttaccccgc
     5881 gagcataaaa agcgtgaatt cgcgcatccg gtacaacaag agagataaac gatgaaaccg
     5941 ttacattata cagcttcagc actggcgctc ggactggcgt taatggggaa tgcacaggca
     6001 gtgacgacca ttccgttctg gcattctatg gaaggggaac tgggtaaaga ggtggattct
     6061 ctggcccaac gttttaacgc cgaaaacccg gattacaaaa ttgtaccgac ctataaaggc
     6121 aactacgaac agaatttaag cgcggggatt gccgcatttc gtaccggcaa cgcgccggct
     6181 attttgcagg tttatgaagt tggcaccgcc accatgatgg cgtcgaaagc cattaaaccg
     6241 gtatatgacg tgtttaaaga ggcggggatt cagttcgatg agtcgcagtt tgtgccgacg
     6301 gtttcaggct actactccga cagcaaaacc ggccacttac tctcccagcc gttcaatagc
     6361 tcgactcctg ttctctatta caacaaagac gccttcaaga aagccggttt agacccggaa
     6421 cagccgccga aaacctggca ggatctggcg gactatgccg cgaaactgaa agcctccggt
     6481 atgaagtgcg gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc
     6541 tggaacggtc tgccgtttgc cagcaaaaac aacggctttg acggcacaga cgcggtgctg
     6601 gagttcaaca agccggagca ggtgaaacac atcgctatgc tcgaagagat gaacaagaag
     6661 ggcgatttca gctacgtcgg gcgtaaggat gaatccactg agaagttcta taacggtgat
     6721 tgcgcgatga ccaccgcctc ttccggttcg cttgccaaca ttcgtgagta cgccaaattt
     6781 aactacggcg taggcatgat gccttacgac gccgatgcga aagatgcacc gcaaaacgcc
     6841 attatcggcg gggccagcct gtgggtgatg cagggcaaag ataaagaaac gtataccggc
     6901 gtggcgaagt tcctcgactt cctcgcgaag ccagaaaacg ctgccgagtg gcatcagaaa
     6961 accggttatc tgccaatcac caaagcggcg tatgacctga cccgtgagca gggcttttat
     7021 gagaaaaacc caggggcgga taccgcgacg cgtcagatgc tgaataagcc gccgttaccg
     7081 ttcaccaaag ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag
     7141 ctggagagcg tgtggaccgg taagaagaca ccgcagcagg cgctggatac cgccgttgag
     7201 cgtgggaacc agttactgcg ccgctttgag cagtctacga agtcttaatt ctcttaccct
     7261 caccctaacc ctctcccaaa gggagagggg accgatcggc gtggtatcct ggtgccgtgt
     7321 cggtttgcaa cttcgctcgg tgggaaagta tgcaccgcct ttctctctcg cccctctggg
     7381 gagagggccg gggtgagggg aacacaacac gaagagttta actatgtcat catcccgtcc
     7441 ggtgttccgc tcgcgctggc tgccctattt gctggtcgcg ccgcagctca tcatcaccgt
     7501 tatctttttt atctggcctg cgggcgaagc gttgtggtac tcgctgcaaa gcgtcgatcc
     7561 gtttggtttc tccagccagt ttgtcggcct ggataacttc gtcacgctgt ttcatgacag
     7621 ctactatctc gactccttct ggacgacgat aaaattcagc acctttgtca ccgtcagcgg
     7681 tttgctggtg tcgctgttct ttgcggcgct ggtggagtac atcgtgcgcg gcagccgttt
     7741 ctatcaaacc ttaatgctgc tgccttacgc tgtggctccc gccgttgccg ccgtattgtg
     7801 gatcttcctg tttaaccccg gtcgcgggct gatcacccat tttctcgctg agttcggcta
     7861 cgactggaac cacgcgcaaa acagcggtca ggcgatgttt ctggtggtgt ttgcctcggt
     7921 gtggaagcaa atcagctaca actttctgtt cttctatgcc gcgctgcaat ccattccccg
     7981 ttcgttgatc gaagccgcag ccatcgacgg tgcaggcccg attcgccgct tctttaagat
     8041 tgcgctgccg cttatcgctc cggtgagttt cttcctgctg gtggtgaacc tggtgtacgc
     8101 cttcttcgat accttcccgg tgatcgacgc cgccacgtcc ggcgggccgg ttcaggccac
     8161 cacgacgctg atttataaga tttaccgcga aggctttacc ggactggatc tggcttcgtc
     8221 tgccgcgcag tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg tgcagttccg
     8281 ctatgttgaa agcaaggtgc gttaccaatg attgagaacc gtccgtggct gacaatattc
     8341 agccatacca tgctgatcct cgggatcgcg gtgatcctct tcccgctgta cgtggcgttt
     8401 gtcgcggcga cactggataa acaagccgtc tacgccgcgc cgatgacgct aatccccggc
     8461 acgcatctgc tggaaaacat ccacaacatc tgggtgaacg gggtaggcac gaatagcgcg
     8521 ccgttctggc ggatgttgct taacagcttt gtgatggcgt tcagcattac gctcggcaaa
     8581 attaccgtct cgatgctctc ggcctttgcc attgtctggt ttcgttttcc actacgtaac
     8641 ctcttcttct ggatgatttt tatcaccctg atgctgccgg ttgaagtgcg tatcttcccg
     8701 acggtggaag tcatcgccaa cctgaaaatg ctcgacagct acgcaggttt aacgctgccg
     8761 ctgatggcct cggcgaccgc cacgtttctg tttcgccagt tctttatgac cctaccggat
     8821 gaactggtgg aagccgcgcg gatcgacggc gcgtcaccaa tgcgtttctt ttgcgacatc
     8881 gtttttccgc tgtccaaaac caatctggcg gcgctgtttg tgatcacctt tatctacggc
     8941 tggaaccagt atttgtggcc gttgttgatt attaccgacg ttgatctcgg caccaccgtg
     9001 gcagggatta aagggatgat cgccacaggc gaaggcacaa cggaatggaa ctcggtgatg
     9061 gcggcgatgt tgttaaccct gatcccacca gtagtgattg ttttagtgat gcagcgtgcc
     9121 ttcgtgcgcg gcctggtcga tagtgagaaa taagatggca ggactgaaat tacaggcagt
     9181 aaccaaaagc tgggatggca aaacccaggt cattaaaccg ctgacccttg atgtggcgga
     9241 tggcgaattt atcgtgatgg tcgggccgtc tggctgcggg aaatcgacgt tattgcgaat
     9301 ggttgccgga ctggagcggg taacgacggg cgatatctgg atcgaccgca agcgtgtcac
     9361 cgaaatggag ccgaaagatc gcgggattgc gatggtgttc cagaactacg cgctttatcc
     9421 gcatatgagc gtcgaagaaa acatggcgtg ggggctgaaa attcgcggca tgggcaagca
     9481 gcaaattgcc gagcgcgtta aagaagcggc gcgcattctg gagctggacg gcctgcttaa
     9541 gcgccgcccg cgtgagctat ctggcggtca gcgtcagcgt gtggcgatgg ggcgtgcgat
     9601 tgtgcgtgag ccggcagtgt tcctgtttga tgagccgctc tctaacctcg atgccaagtt
     9661 gcgtgtgcag atgcgtcttg aactgcaaca gctgcaccgt cgcctgaaaa cgacttcact
     9721 ctacgttacc cacgatcagg ttgaggcgat gacgctcgcc cagcgagtaa tggtgatgaa
     9781 cggcggcgtt gccgaacaga ttggcacgcc agttgaagtg tacgaaaagc ccgccagcct
     9841 gtttgtggcg agttttatcg gcagtccggc gatgaatctg ctgaaagggc gtgtgaataa
     9901 cgaaggtacg catttcgaac tggacggcgg tatcgcgctg ccgctaaacg gtggctaccg
     9961 tcagtatgct ggacgtaaaa tgactctcgg cattcgcccg gaacatatta cgctacgttc
    10021 gcaggctgaa ggtggcgtgc cgctggtgat ggacacgctg gagatcctcg gcgcagataa
    10081 cctggcgcac ggacgctggg gcgaacagaa gctggtgttg cgactggcgc atcaggagcg
    10141 cccgacggca ggcagcacgc tgtggctgca tctgccggaa aatcagctac atctttttga
    10201 tggtgaaaca ggacaacgag tatgagtaac tggccttatc cccgcatcgt cgcccatcgt
    10261 ggcggcggta aactggcccc ggaaaacacc ctggcggcaa tcgatgtggg ggcaagatac
    10321 ggtcataaga tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat cttcttgctt
    10381 catgacgaca atctcgaacg caccagcaac ggctggggcg tcgcgggtga actgaactgg
    10441 caggatttac tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa aggtgagccg
    10501 ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat ggcgaatatc
    10561 gaaatcaaac ccaccactgg caccgggcca ttaacgggca aaacggtggc gctggcggca
    10621 cgcgaactgt ggaccggtat gacgccgccg ctgctgtcat cgtttgagat tgatgcttta
    10681 gaagccgcgc aacaggcagc accggaactg ccgcgtggtt tattgcttga tgaatggcgc
    10741 gacgactggc gagaattgac cgcgcggctg ggctgcgtct ctattcatct caatcataag
    10801 ttgctcgata aagcgcgagt gatgcagttg aaagacgccg gattacggat tctggtttat
    10861 accgtcaata aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga ttgcatctgt
    10921 accgatgcga ttgacgtgat tggtccgaac tttacggccc aatagttttc aacggaatgt
    10981 caggctgcgg cgtgctgggc tgattcagca tgtcgccgtt tctcctttcc ggcagcatat
    11041 gctgctgatt caacgaacta tccggattac ggttgctgtt taacatcccg ccattggtgt
    11101 tgggcagcat ttgctgtcgg gcgggatttc gctcccccgg ctgtgactgc aacacccgct
    11161 gagaattatt gtttatctgg ttttctaaat gctgctgttg cagctgcgtt tgcgttttca
    11221 gttgctgatt cagcatccct ttttgctgga tttgctgcgt ttgcatttgg gtttgcatcc
    11281 gctgctgact ggggatctga taccccggct ggttagggtt attcagagta ttaatgggct
    11341 gtgcaaagcc gacaaacggc aggagtgccg taagaatcag aagtcgtttc atcgcgtatc
    11401 ctcctctgaa gatattcttt aagtttactc ctttcctgca atcatcagga ttaattcaga
    11461 gttatatacc aggcttagct ggggttgccc cttaatctct ggagaataac gatgataaaa
    11521 ccgacgtttt tacgccgggt ggccattgct gctctgttca caggaagttg ttttagtacg
    11581 gtagccgcgc cacctgcgcc gtctcctgtt tcttacggcg tggaggaaga tgtctttcat
    11641 ccggtacgcg cgaaacaggg gatggtggcg tctgtagatg ctactgccac tcaggtgggg
    11701 gtggatatcc tcaaggaggg cgggaatgcc gttgatgccg ccgtggctgt gggctacgcg
    11761 ctggcggtaa cgcatccgca ggcagggaat ctgggcggtg gtggttttat gctgatccgc
    11821 tcgaaaaatg gcaataccac cgctatcgat ttccgcgaaa tggctcccgc caaagcgacc
    11881 cgcgatatgt tccttgatga tcagggtaac ccggacagca aaaaatcact cacttcgcat
    11941 ctggcttctg gtacgcccgg tacggtagca ggtttctcgc tggcgttgga taaatacggc
    12001 accatgccgt tgaacaaagt ggtgcaaccg gcgtttaaac tggcacgcga tggttttatc
    12061 gttaacgacg cgttggctga cgatctcaaa acctacggta gcgaagtgtt gccgaatcac
    12121 gaaaacagca aggctatctt ctggaaagag ggcgcgccgc tgaaaaaggg cgacaagctg
    12181 gtgcaggcga acctggcaaa gagcctggag atgattgccg aaaacggccc ggacgaattc
    12241 tacaaaggca cgatagcgga acagattgcc caggagatgc agaaaaacgg tggcttgatc
    12301 actaaagaag atttagcggc gtataaagcg gtcgaacgta caccgattag cggcgaatat
    12361 cgcgggtatc aggtttactc gatgccaccg ccatcttccg gtgggattca tatcgtgcag
    12421 atcctcaata tcctggaaaa cttcgatatg cagaaatacg gctttggcag cgccgatgcg
    12481 atgcaaatca tggcagaagc ggagaaatac gcctacgccg accgctcgga atatcttggc
    12541 gatccggatt ttgtcaaagt cccgtggcag gcgctgacca ataaagccta tgccaaatct
    12601 attgccgatc aaatcgatat caataaagcg aagccatcca gcgaaattcg ccctggcaag
    12661 cttgcgcctt atgagagtaa tcaaactacc cattactcag tggtggataa agatggtaac
    12721 gcggtggcgg tgacctatac gctgaacacc accttcggta cgggcattgt cgcgggcgag
    12781 agcggtattc tgcttaataa ccaaatggat gatttctccg ccaaaccggg cgtgccgaac
    12841 gtttacgggc tggtgggtgg tgacgctaac gcggtcgggc cgaataaacg tccgctgtcg
    12901 tcgatgtcac caaccattgt ggtgaaagac ggtaaaacct ggctggtcac cggaagccca
    12961 ggcggtagcc ggatcatcac taccgtgctg caaatggtgg tgaatagcat cgattatggt
    13021 atgaatgtcg ccgaagcgac caatgcaccg cgtttccacc atcagtggtt gccggatgag
    13081 ctgcgtgtcg agaaaggatt tagcccggat acgctcaagc tgctggaagc aaaaggtcag
    13141 aaagtggcgc tgaaagaggc gatgggcagt acacaaagca ttatggttgg gcctgacggt
    13201 gagttgtacg gcgcatccga cccgcgctca gtagatgatt taacggcggg gtattaaggt
    13261 tagcggccct cttcgtggga agagggccgt attgtcaggg caagccgaag gtagcctttt
    13321 ttatttcata atcctgtaga tattcttcca gcggctttgc cgttccaagg gaatgaattt
    13381 caccactgtc tttatcaata ataaaaggtg cgttaccggc taagcgcgcg gcctcatcac
    13441 cagattcaag aaattcttgt gattcgaaac agaaatacca gccctggata aaacgtccat
    13501 gcagagtgat gacgacaggg agatctgcat catcaaggta atggttcgct ttcgcgaatg
    13561 cgtcgtgata agtaatcata attataataa atattattgt tgagcgttat attattattc
    13621 ttgcgttata ctttgagaaa aggcgtgttc atcttcgtca aagatattat taaatgctgg
    13681 ttttgaaaaa agttcatcgg ataatataca gtcacgaaat aagtatctgc taatattttg
    13741 tgatttcttt ttcagaacgt cgaacgggat tttcttattt aagatatcag aatcttatga
    13801 ttcgggtgaa attctaacta aatcaacatg tcactaaaat catcatagcc attagctatt
    13861 tcggctaaaa tagagattac atgtcttcgg cccatcttac ttaaggagtg tagttccgtt
    13921 gtaagttttt ccatagcttg catttgctaa gtttcgaaca aggaattttc tgttggtaat
    13981 ctctaaaaag atggcatggt ttacaatgat ttttgttacc ttttgattat tatgaacaac
    14041 tgtccatgat ttcgtttaag aatg
//
LOCUS       DAZGTJ010000114        13945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000114 DAZGTJ010000000
VERSION     DAZGTJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.114"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHK8E_004365"
     CDS             complement(<1..242)
                     /locus_tag="ACHK8E_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0346549.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSG"
     gene            1583..3073
                     /locus_tag="ACHK8E_004366"
     CDS             1583..3073
                     /locus_tag="ACHK8E_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0346550.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(3195..3767)
                     /locus_tag="ACHK8E_004367"
     CDS             complement(3195..3767)
                     /locus_tag="ACHK8E_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346551.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(3853..4059)
                     /locus_tag="ACHK8E_004368"
     CDS             complement(3853..4059)
                     /locus_tag="ACHK8E_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0346552.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            4916..5140
                     /gene="hha"
                     /locus_tag="ACHK8E_004369"
     CDS             4916..5140
                     /gene="hha"
                     /locus_tag="ACHK8E_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP0346553.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(5472..5669)
                     /locus_tag="ACHK8E_004370"
     CDS             complement(5472..5669)
                     /locus_tag="ACHK8E_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346554.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            5868..6437
                     /locus_tag="ACHK8E_004371"
     CDS             5868..6437
                     /locus_tag="ACHK8E_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0346555.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            6698..7099
                     /locus_tag="ACHK8E_004372"
     CDS             6698..7099
                     /locus_tag="ACHK8E_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0346556.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            7087..7521
                     /locus_tag="ACHK8E_004373"
     CDS             7087..7521
                     /locus_tag="ACHK8E_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP0346557.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            8296..8522
                     /locus_tag="ACHK8E_004374"
                     /pseudo
     CDS             8296..8522
                     /locus_tag="ACHK8E_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(8524..9306)
                     /locus_tag="ACHK8E_004375"
     CDS             complement(8524..9306)
                     /locus_tag="ACHK8E_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFP0346558.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            9609..10529
                     /gene="rbsK"
                     /locus_tag="ACHK8E_004376"
     CDS             9609..10529
                     /gene="rbsK"
                     /locus_tag="ACHK8E_004376"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0346559.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            10557..11873
                     /gene="fucP"
                     /locus_tag="ACHK8E_004377"
     CDS             10557..11873
                     /gene="fucP"
                     /locus_tag="ACHK8E_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0346560.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            11885..12898
                     /locus_tag="ACHK8E_004378"
     CDS             11885..12898
                     /locus_tag="ACHK8E_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP0346561.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            13320..13604
                     /locus_tag="ACHK8E_004379"
     CDS             13320..13604
                     /locus_tag="ACHK8E_004379"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0346562.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(13820..>13945)
                     /locus_tag="ACHK8E_004380"
     CDS             complement(13820..>13945)
                     /locus_tag="ACHK8E_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0346563.1"
                     /translation="LLITFLPVFLLPVLGDIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT         3633 a         3054 c         3489 g         3769 t
ORIGIN      
        1 ccactcagac ggaagcgccg tacttcccgg gtgccggcgt gaacatcact gaccggggtg
       61 acctccccct gaaacagtgc attacagagt gaggatttac cggccccgct tttacccata
      121 atgccaatca cgggttcgtg actggtgagt ttacgcagat gttccaggat gtgacgggaa
      181 agtgagtaag gcagggagga aagcggtttt tcaattgcct caatggcatc agacggattc
      241 atacatgtat tcccggtgaa aaaagacaaa accccgcgca ccggaaaggt ggcgggggat
      301 ccacggagat aatatctgtc tgaaattgtt ttgaatcgat gggattgttg cgtattaaca
      361 tgaaggagat caggacttat ccgttactgg gtgatctcct tccatgaaaa tacgcggtta
      421 ttgttaaaca gcaggctgat tactccgtaa aggagaaaaa tattcatctt gcagcctgat
      481 tatcaggtgt gtttatgttt actgaggtga gtatttttca gtatattgtc ggggcagtca
      541 gtgggagata ttaaatttac gtttagagtt atcttgcttc tgctgtgtat ttttataaga
      601 gtgcgcacgt aatgatatct gttgatacca tgttgttaag tgtttccctt ccgggtcatg
      661 cgtacttgca aaatggcatc tgaatcgatc acggtttggc caaaccgtga tcgaatagag
      721 aggaatacgc acgtaatgat taaatgcttt tgttacacca ttcttccggc atgaatattt
      781 atccagttcc cttttttatt tgtgtagtga tactgtcgga atagcgttca gtaatgtgaa
      841 gtgtcctgtt aaaaatcttt aataaatgtg tcaccctggt caggaaatat ctgctgagat
      901 attattagtt ggtggtgttt ttactgagta atagctggct tttaattcct gaactgaaat
      961 gctgtcgctc gtcagggttt aattaaaact ggatgatgat gtgaaaatga aggctggttt
     1021 ttgtgtttgt cgaggaagtg ttgtttttat ccattctcat cattggctgg ctgcgattat
     1081 acagaagtat ttgaagaaaa aatggtgcat tttatctgtg ccggcaggga ggaggatttt
     1141 cctgatatgt cagtattaaa tggtttttgt tatttataaa aatgtcatcc tgtccataga
     1201 atattgtact gagtactcag cattcagcgc aggaactgtc atcttcttta ttttattctt
     1261 cttcaggagg gcagttttct gaaagcgtta ctccggggaa actgtttatg tttattcccg
     1321 gcaggaaacc cgtacctgca ataccctgga tgatgttttt tgctggtacg ttgttgtgtg
     1381 aaaattaatc aacatgtgtg ctccggagag cgcccgtaga cgcacagatt cattctgaca
     1441 ttctttagcg acaaaatacc gggtggttat tatttaacct gaaaatcgac agaaattttt
     1501 atcctgtatt gattctgctg ctatttttgc tattagtagc aatagttgcg gtactttatg
     1561 ttctgtgtgt tgtgaggtca ttatgccata ctttcagtat cctgatgagt ttcccctgag
     1621 ctcgctgcct ccactcattc gtgatgccgt tattgaagct cagcaaatca cgcaggctcc
     1681 cctggggctt gtggcagcat ctgccctggg cgctgtttca cttgtctgcc agaatctgat
     1741 tgatgtctgc agactgaata cactgcgggg gccggtttct cttttcttac tgacgcttgc
     1801 tgagtccgga gaacgaaaaa ccgcagtgga caaattatta atggagcccc tgtaccagca
     1861 ggagatgctg ttatattcca gacacaaaaa tgaactgacg acatggaaaa ataaggaaga
     1921 gcttttaaag gcgcagaaaa aagcattgtt gtcaaaactg aataaagaac tccgcaaagg
     1981 cgcggatgag tcagaaacgc tcagacaact ggaagcgctt cagaaaaaca gaggggaaaa
     2041 acctgtaaga tataaattca tttttaatga tgccacgaca gcagcaatta aagatcagct
     2101 ttgtggtcag tggcgttcag tgggtatcat gtctgatgaa gccggtataa tatttgatgg
     2161 gtataccctg tctgagctgc cttttattaa caaaatgtgg gatggctccg tattgtcggt
     2221 ggacagaaag aatgaacccg aacagatgat tgaaaatgcc agaatgacat tatccctcat
     2281 ggtacaaccc ggtctttttg accgatatat ggagcgaaaa ggttctgttg ccagggacag
     2341 tggttttctt gcccgttgtc taatcagtaa acctgccacc acgcagggga aacgctttat
     2401 taacggagca gtgaccccgg gagaatcatt aacggcattt catgaacgat taatggaact
     2461 ggccagaggc agtattgaaa aatccagtaa ggatgaacgt tattgtctgc atttttctcc
     2521 agaagcgcaa aaaatattta ttgatcacta taatgttctg gagcaggatt tgtctccttc
     2581 cggaccctta agccagtttc gggggcatgt ttcaaaaaaa acagaaaaca ttgcgagaat
     2641 tgctgcgtta ttgcagtatt tcagtcaggg tgaagggaaa atctctgccg atatcatgac
     2701 gtctgctgtc gtaataagtt cgtggtacac agatgaatat aaaaaattat ttgcgttacc
     2761 tgatgaatct gaactgcagc tgcaggacgc ccgggagctg ctcgactggc tcattgagga
     2821 gtgccgcgga gaatgcccgc cccgtgtcag gaaaaattat attctccagt gtggcccggg
     2881 ccggttcagg aacaggaaaa agctgaacgt cctgctgaac attcttgcat ctcaactcag
     2941 gctctctgtc gtgcaggaag gaaaaaccac ctatgtcctg ctttccgaaa taaacaatat
     3001 cacgctgcct gaactgaatg cgtgctacag ccagaaactg ataagatggc atgagcaatc
     3061 ctttggctta taatgttccc tgttgctgag acggattatt cagtggtggt atcgcagtat
     3121 gcccgtcacc ccccgcttct gcatgagggt gacggaggaa aagtaagtgt gcggaggccc
     3181 gggagcgtta tgaatcaaca ggcgtataac aggtactcgt caatcatcag ttccagctcg
     3241 cgcttttccc gtttgcgcgc cacagcccag ttttcaagct ctgcatgtga tttatgcttt
     3301 aactcttcag tccagggctg catggagtgg gcacacatca gatcatacca gcacagccat
     3361 atcagcgtgt ggtagagatc acgatcgtcg aaacgccgga gcaggctgtt cagcctgttt
     3421 gccagccggt tttcacccag acgatgtagc tcactgagta aaacgccgcc cagattaaaa
     3481 tgtgtaagca gcatgatttc atgggtctga atgctggtca ttaacccttc ctgaatggca
     3541 ggaatgaacg gtggtgttcc cgggttgacg acctgataga gtccctgtgg tgccactgag
     3601 atgatagtgc ctttttccgg cagaaaaaag tgggccaggg tcaggcgatg ataaccatga
     3661 agatgcggag accccattcc atgagcgacg ataagcctcg tcacgcttgt catccatttc
     3721 tgatgctgga tgagcagcag ctcacaccgg ttttcaagtt tttgcataat attctctcct
     3781 ttagcgcgca gcaaaatgcc tccgggggcc ggatcatgcc tttgctgcgg ttgaacggat
     3841 aacggtcagg aattacggac gggactgtgc aatacgcgcc tgcagccagg tttccacttc
     3901 acttttcagc catcgggagc tacggcccag tttgatgggg gcaggaaagg caccatcctt
     3961 gatgagcttg taaaaccatt tatcggtcag gccggtcagt tgagtgataa atgccatgtc
     4021 gaccatctgg tcatccatca gtgaaactgg ggtattcatg agaatgttcc ttcggatgtt
     4081 gttaatctca accggcgttg gtgatgaaaa caacgcacca ccttcaccgg cataacaacc
     4141 ggggagaaac gtaaaaaata atcgggaatg tgaccgtcag gagataaacg gggggattgc
     4201 ggtggctgag ccgtaccggt atgtcgggtg attgttgcga aacgatgctt ccgcacgcgc
     4261 acgggaggac tttcgtgaga caaggggaaa actggcgttc tgataattgc agagttgttt
     4321 ttgaaggcgg ttgtggtgca tcagcctgtg acctgtgggc aggcgacagg tatcactggt
     4381 gggataagac tgtctgattg gtattataaa tatataagag agctcaaaca catgagttca
     4441 tatatgtgaa ataaatctgt gagtgtgatc agcagaccag tctacccagt agcaacagat
     4501 tgcgttgtac tcacagggtt atccctgata actggcgtat aacctgcgta cataaaacgt
     4561 acctgcaatg cacctgaaca gagtgcatta agcatccgcc tgagcggtaa acgtatccga
     4621 tctggtgagt gacagtctga gagcattctc agtaatcgcc cccttgtatc ctggaaattg
     4681 gttaagagcg attagatttg gcaaggtgta tgcatccagc agcttaacac ctcagctaac
     4741 tggctggtta ccttcaggtt gtcgcaatgg catcggacat ccggtgtcgg ataactaaac
     4801 gtagcgttaa ggcgatattg ctgagagacc ggttaagtgg tggcacggga gtaatgagtt
     4861 tggggagggt accagaatgc cagtcaggtg cggtatgctg gcagtatcac gttgattgca
     4921 gcataaagta acgagagacg aatggaagtt aaaaccagag cagttccgtc gttgttcatc
     4981 ccgggagacg ctggaaaaag tgattacccg cacacgttat aaacttaccc ctgcggagct
     5041 ggaagccttt aactctgcgg tcgatcaccg gctggcagaa ctgacaatga acaaacttta
     5101 cgatcgcgtg ccggcttccg tctggaaata tgtcacctga atcttgaatc cgcgctacga
     5161 tgactctggt gttatctgtg caccggaaga tcgggaacat taagcagtat aacggcaaca
     5221 aagaattcga aatcaaaagg gaatggtgta cagaggggca ggcgttcagg cattaccggc
     5281 ctgtatcttc tgaaggggac tcgtatttgt cggggctgag tattccgctc gattcgatca
     5341 cggattggca gaaacctgac tgtgattgat tcaggtacca ttttgcaggg atccggtgcg
     5401 ttactgtctg gcacaacaaa cgcagcgggt cgctctgccc cgccgattcc tgataattcc
     5461 tcccctgtaa cctactgcaa cagtacaaat acactgacgc ctgaaacggt aaccagaatc
     5521 agcaccagca gtaacgtcag taaacagagc agacgatcgg tcttccggta ataaccgaag
     5581 gggtcgacag cgccacaata cgggcaacac actgtattat gagtaattgc gtttccacag
     5641 tccctgcact tctttttgcg tatcacgatc gctccctctc cacgccttat ccgtaacctg
     5701 ttttttacat taaaaaaaca acgccgggaa ttcaatcgtc agtccggaga cggccgttcg
     5761 ggttatcaca gagtgcctga gacagtgtcc tgccggaggt cacctcaggg gctctgcgca
     5821 ttttttacga cgtggctatt ccgtatgaac catacggaga tttaaccatg acctacaaat
     5881 acaacccctt ctggcagcaa cgtattcgtg agacggtgcg gcacgcactg gatgttcatc
     5941 cccgcctgac ggcacttcgg gttgatctgc gtctcccgga tgtgccggca gcaacggacg
     6001 cggctgtgat atcccgcttc atcaatgccc tgaaagcccg aatcgacgct tatcagaaac
     6061 gtaagcatcg ggaaggtaaa cgcgtgcatt cctcgaccct gcattacgcc tgggcccggg
     6121 agttcgggga gcttaaaggc aaaaaacact atcacctgct gctgctggtc aaccgggata
     6181 cctggtgtcg tgccggcgat taccgcgctc cggggtcact ggccgggatg attaaacagg
     6241 cctggtgcag tgccctggga gtggatgccg gacgttatga cacgctggca cattttcctg
     6301 acaagccagc ggtgtggctg gaacgcgatg atgaaaccgg ctttcagcag gtgctggaac
     6361 gttctggcta tctggcgaag gagcatacca aagctcgcgg caccggtgag cgcaattttg
     6421 gctgtagccg gagttgaaca cgacaggcgc accaataccc ggcaaaccaa aagtatgggt
     6481 tgttgtctgg cggcaacgat gtatccactc gcccactgac tgttttttac gtcagtgggc
     6541 cattgtgtga caaccacagt gctgtaacag caaccagcct gctttaccca aactccagtc
     6601 cagattttaa ctgaaccgcc attcacaccc tgaccacgct gcccgtaccg ggacggctgt
     6661 tgcctgcgtg tcgtctggcg gctgaggaac atatactatg tacgcaaaat cctttctcgc
     6721 tcttgatggc aacggacgtc tgacgggggc ccgtaccgca cagactgcgc cttacgaccg
     6781 ctacacctgc cacctttgcg gcagtgcact caggtaccac ccgcaatacg acactgaacg
     6841 tccctggttt gaacacactg acgacgggct gactaagcac ggtcatgagt gcccttatgt
     6901 cagaccggaa cgcagggagg ttcgcttgat taagcgtctg cagcaattcg taccggatgc
     6961 cttacccgtg gtgcgtaaag ccagctggca ctgcagacaa tgtcaccacg attattatgg
     7021 ggagcagtac tgcacaaact gccagaccgg aggattcagt attccccgga caactcagga
     7081 ggaaatatgc gaattctgaa ctgttatatg gcgaatgaca gcaaaggcca ttttgttacg
     7141 gcgaaagaag ctgcgaagca caaccgacag gacgttttgt gctgtgtgtc ctgtggatgc
     7201 ccgttaacac ttcagcgggg caatgacgga caaccaccgt ggtttgaaca tgaccagatg
     7261 actgtcgctg aaaaaatcct gctgcgatgt acctggcttg acccggcaga gaaagaggcc
     7321 cgtcgtttgc atctgcaggg catgacggtt ccggattata cggtgaaggt gagaaagtgg
     7381 ttttgtgtga tgtgtgacga agattatgag ggggaaaagt gctgcccacg ctgcggtact
     7441 ggggtataca gcagggcgtg ggggcggcag gaggtgccgt cggaagatgc cagggctgat
     7501 aatccgttac agaggctgta atggttgcct ccggagcagt ttgcggtaat gccgttcccg
     7561 ggaatggatt gccagtgcgg ggactgtgga gcttaattcc ggtgttggtg gcctttccgt
     7621 tgattttgag ccaacagccc tcctgtgact gacaggctgc ctcgtcattc cattcgtatc
     7681 tccagtaaca gggggttgta ttgtatttta atgtgccggt tcaggcgtgc gtttcccggc
     7741 gcgtctgcac cggcttaacc aaattcaaca gggataaaat agtgtaaata accgaagtat
     7801 tacttgcaga atcgggggaa gcatccgggg ttttattcaa caggcatggg aggctccggc
     7861 tctgacagac attgtactgt ggaatgttgt tacaggttgg ggcggcaacc gtgccaggag
     7921 accatgagcc gttcacggca ttaaaggcca cacttcggtc gtttacattc cctgtggtgg
     7981 caggcatctt ccgttaaggc gtcagagcgt taccaacgtt atgttgtcat ggctcacctt
     8041 tcatagtcga tgcttatctg gtgactgaaa ctagccgcag ataaagagtg tgaagttgat
     8101 accatggagt ggagtgacgt gcaggcagga gattcgctct atgcagggtt gtgagatgaa
     8161 ttcaaaccgg ctgtcagcgc cagtaatatt tgaagattcg tccggttgtt atccagtgtg
     8221 cattaaaaac ccagacatta tggacaatac aacggcttcc ataacaggga ggagcctgtt
     8281 tattatccgg gcgtaatgac taacctttgt gggctttttc ccgtcgtgct tttaacgttg
     8341 aaggcatttt ctgcctgccg attcaggaca gcgacaaaag ccatatcaga caattggtca
     8401 atgatgacca gcgtctggag tagatgataa gccaaattga taaactggaa tatgtgatga
     8461 aagtgcaacg taatcagtcc aatccgacga tgtttaacaa gagtacggtg ttttttcagt
     8521 aagtcattcg tcttctgaat caggtgtaat gatctttaca gaagcattcc ggcagtgcgt
     8581 aacataatct gccggtaacg gacgctcgct aatcagaaca tcaaatgcag agagcggtgc
     8641 gaggctggca gaggctactt catcgaacaa ggcgtggcgg gcgagcaaaa ttttccttag
     8701 tccacgggcc atcgctttac gcttagtggc aagatcttcg ggattaaacc agctgacgcc
     8761 aaagtgatca tgtacaccgc tggcggaaat aaatattttt cgcggattga gagagtctaa
     8821 tggcgaagag ttactggcat cgtaaaaagc atcacttctg gcacgatacg taccaccaca
     8881 aagtattgct gtcacattag gtttttcatt caacgcaaca aagacacgat gtgagtaaca
     8941 gatgccagtg aaggtgattg catccgggat catgcttata acgagcggta tctcctggcc
     9001 attatcaaag aagatcagat cattttcatt aaccattccg gcggccagaa ttgcaatagg
     9061 taagtcatca cgatgatttc tcggaacgtc ctggattact ggcatggatg gtgcgggttt
     9121 atgcaccatt acaatatagc cacccagtag ggtcagtggc agaggttcat cttcctgatg
     9181 gagatcgcga cgaatagtca ttacagaaac ttccagcatt cgtgccgcgt ctttcaaatg
     9241 gattctgtcg gttttcttaa gtatttcaat caaacgtcgg atacgctctt tttgcttcgt
     9301 ttccatcaac tctctccgta aaatgttata aaaataacat tatggcatta gtaaacattg
     9361 ttttagttac catactatac gaccgaccct ttgaaatgaa aagggtatag actgctgaat
     9421 tttcggtgtt aatcctgtta ggttgcttat gattagatag tgttggcctg agtgataaga
     9481 ctggaagtct attaaataaa catcgggttg atgtgctaat cgcgatctca gtcacaaatg
     9541 atgcaaaagt aacatgctaa tgttatttaa ataccattgt tatgcgaaag tttataagag
     9601 gtagtgaaat ggacattgca gttattggtt ccaatatggt ggatctcatt acttacacta
     9661 atcagatgcc taaagaagga gaaactctgg aagcacctgc cttcaaaatc ggatgtggtg
     9721 ggaaaggtgc taatcaagcg gtggcggcgg ctaaattgaa ttcaaaagtc ctgatgctga
     9781 ctaaagtagg tgatgatatt tttgcggata atactattcg taatctcgaa tcatggggaa
     9841 ttaataccac ttatgttgag aaagtcccct gtaccagcag tggtgttgca ccgatctttg
     9901 ttaatgccaa ctcgagcaat agtatattaa taattaaggg agcgaataaa tttctttccc
     9961 cggaagatat cgatcgagct gcagaagatc tgaaaaaatg caaattgatc gttttacaac
    10021 tggaagttca actggaaaca gtttaccacg cgattgagtt tggtaagaaa aatggtattg
    10081 aagtgttatt aaatccggct ccggcattgc gtgagttaga tatgtcttat gcctgcaaat
    10141 gtgatttctt tatcccgaat gaaacagaac tggaaatatt aaccggtatg tcggttgata
    10201 cttatgatca tattcgtttg gccgcacgtt cgttagttga taaagggctg aacaatatta
    10261 ttgttaccat gagcgaaaaa ggcgcattat ggatgacccg cgatcaggaa gtccatgttc
    10321 ctgcattcaa agtaaatgct gttgatacta gtggcgctgg tgacgcattt attggttgct
    10381 tttcccatta ttatgttcaa agcggtgatg ttgaagccgc tttgaaaaaa gccgctctct
    10441 ttgccgcttt cagcgtcaca gggaaaggta cgcaatcatc ttatccgagt attgaacaat
    10501 ttaatgaatt tttaacctta aacgaataat catcctgcac agttaaaggt agcactatga
    10561 acgataaaaa catcattcag atgcctgatg ggtatctgaa taagactccc ttgttccagt
    10621 ttattttgtt atcgtgttta ttcccgctat ggggatgcgc agctgcatta aatgacatat
    10681 taatcactca attcaaaagt gtattttcgc taagtaactt tgcttcagca ttggtgcaga
    10741 gcgcatttta tggaggttat tttttaattg caataccagc atcccttgtg attaaaaaga
    10801 ccagttataa ggtggcaatt ttaattggcc tgacgctgta tattggtggt tgtacacttt
    10861 ttttccctgc ctcgcatatg gcaacctaca ctatgttcct tgcagctatt tttgcgattg
    10921 cgattggcct gagtttcctt gaaactgcag cgaacacata cagttcaatg atcggtccaa
    10981 aagcctatgc cacattgcgg ctcaatatca gccagacctt ttatccgatt ggtgctgcgt
    11041 ctggtatttt attgggcaaa tatctggttt tctccgaggg ggaaagcctt gaaaagcaga
    11101 tgtcaggaat gaacgccggg caaattcaca actttaaggt gctgatgtta gagaacaccc
    11161 ttgaaccgta taagtatatg attatgattc tggttgtggt catggtgttg ttccttttaa
    11221 cccgcttccc aacctgtaaa gtggcacaga caagtcacca taaacgcccg tcagcaatgg
    11281 atacgctgcg ctatctggct agaaatccgc gctttcgtcg cggtattgtg gcgcagttcc
    11341 tttatgtggg tatgcaggtc gcagtatggt catttaccat ccgtctggcg ctggagttgg
    11401 gcgatatcaa cgaacgtgat gcttcaaact tcatggtcta tagctttgcc tgcttcttta
    11461 ttggcaagtt tatagccaat atccttatga cgcgttttaa tccggaaaaa gtattgattc
    11521 tctattccgt tataggggcg ctgttcctgg catatgttgc actggctcca agtttcagcg
    11581 cggtctatgt cgccgtcctg gtaagtgttt tgtttggccc ttgctgggcg acgatctatg
    11641 caggaacact ggatacagtt gataacgaac atacagaaat ggcaggagca gtcattgtta
    11701 tggctattgt cggtgccgcg gtggttccgg caattcaggg atatattgca gatatgttcc
    11761 actcactaca gctttcattc ctggtttcta tgctctgctt tgtctatgtc ggagtctact
    11821 tctggcgtga aagcaaagta cgtaccgcac tggctgaagt cacagcgtcc tgaggagaac
    11881 cgttatgagt acgcgtatta atttgtggcg ggcgcttttt ggcgagaaac ctcgtatctt
    11941 gctggaaaat agtgatttta ctgtcacgtc ttttcgatat gacagcggtg tcgaggggct
    12001 taaaattgca aacagtcgtg gacatttgat tattcttccc tggatggggc agatgatttg
    12061 ggatgcgcaa tttgatggac acggcctcac catgtgcaat atgtttcgcc agcctaaacc
    12121 ggcgacagag gtgatagaga cctatggctg ttttgctttt cattcaggtt tgctggctaa
    12181 tggctgccca tctgcggaag atactcatct gctacacggt gaaatggcct gtgccgcgat
    12241 ggatgaagca tggatggaat tagagggaga tatgttacgc cttaccgggc gttacgagta
    12301 tgtaaagggg ttcgggcatc attatctggc tcagccaaca gtggtccttc acaaatcctc
    12361 tacactgttc gatattaaga tggccgtaac caatctggct tctgtagata tgccactaca
    12421 atatatgtgc cacatgaatt atgcatatat accaaatgct acgttcagtc agaatatacc
    12481 ggacgaaata ttaaggttgc gtgaatcagt accttcgcat gttaatccca ccactcagtg
    12541 gctggcgttt aatcaacgga ttatgcaggg agaagcctct ctgtcgacgc tgaatcaacc
    12601 ggagttttat gatccagaaa ttgtcttctt tgcagacaaa ctggacgctt acacagatca
    12661 gccagagttt cgcatgatcg ctcccgatgg cactacgttt gttactcgtt tctccagtgc
    12721 cgaactaaat tatgtgacac gctggatcct ttataacgga gagcaacagg tagccgcctt
    12781 tgcattacct gcaacctgtc ggcctgaggg gtatcttgcc gcgcaacgta atggcacgct
    12841 gattcaggtt gcaccgcagc aaacgcgcac ttttacagtc accacaggaa ttgaataacc
    12901 actcaaggcc tgaacagcgg cgcgcttatc gttaaggtaa gcgcgttttt ttacccgcca
    12961 ggacaagact atgatagcgt tgcccttata gacgatcaca ttatcgtcca ctccaaattt
    13021 gcgcaactac tgtagagtgg tgtactccac agcttctttg aggagcttaa cctccattgt
    13081 tttcttaccg agcaagcact gcaactcttt tatttatttg tagtggtcaa caaaaactgg
    13141 tcacaatttc agtttttttc cagtatctat tttccgattc gttcggcggt aatccaccgt
    13201 tatattcatg aggccggagt gcactataaa agtgtatgcg taaaccgact gctgtatgta
    13261 gccattaagc cagtattggt aacataggta cccacctttt caactagtgg acacctgtta
    13321 tggaacaaaa aacattatca gctgaacccc gcaggtcatt ttcaaatgag tttaaacttc
    13381 aaatggttaa actggcttta caaccaaggg gttctgttgc ccgtattgcc cgggagcatg
    13441 atatcaatga taatcttctg ttcaaatggc tcaggctctc gctgatcaaa gggcgtatat
    13501 cacggcgtct tccggtaacg aactcttctg gcattggcgt tgaattatta cctgtggaga
    13561 tgacgctgga tgagcggcct taccccgttt tttacgcgag gtaattatga agatcaatga
    13621 ctagccgtcg aaccgtgtgc atgaactgct gcactgaaac ttctcagctg taaaataatc
    13681 cttacgctac gtacttcccg gagcgcttac gattaatcgg atgcagtcct gactggaaga
    13741 aagaaaaatg gagaaaataa gaggaattaa tatcctccac gttcctgaaa tcctggagga
    13801 tattaatgtc atcaaaatat tagcgaacga taatccctaa caaaataccc agtgcgccaa
    13861 agtcagcatc actgaaggtg gtgttagcaa aaccaatatc acccagcacc ggcaataaga
    13921 atactggcag gaaggtaata agcaa
//
LOCUS       DAZGTJ010000115        13216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000115 DAZGTJ010000000
VERSION     DAZGTJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.115"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            90..1814
                     /locus_tag="ACHK8E_004381"
     CDS             90..1814
                     /locus_tag="ACHK8E_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP0346564.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            1811..2725
                     /locus_tag="ACHK8E_004382"
     CDS             1811..2725
                     /locus_tag="ACHK8E_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0346565.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2757..3005
                     /locus_tag="ACHK8E_004383"
     CDS             2757..3005
                     /locus_tag="ACHK8E_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0346566.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3005..4177
                     /locus_tag="ACHK8E_004384"
     CDS             3005..4177
                     /locus_tag="ACHK8E_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0346567.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4212..5291)
                     /gene="lptG"
                     /locus_tag="ACHK8E_004385"
     CDS             complement(4212..5291)
                     /gene="lptG"
                     /locus_tag="ACHK8E_004385"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0346568.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5288..6358)
                     /gene="lptF"
                     /locus_tag="ACHK8E_004386"
     CDS             complement(5288..6358)
                     /gene="lptF"
                     /locus_tag="ACHK8E_004386"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0346569.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6388..6948)
                     /locus_tag="ACHK8E_004387"
     CDS             complement(6388..6948)
                     /locus_tag="ACHK8E_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0346570.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6960..7784)
                     /locus_tag="ACHK8E_004388"
     CDS             complement(6960..7784)
                     /locus_tag="ACHK8E_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346571.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7784..9133)
                     /locus_tag="ACHK8E_004389"
     CDS             complement(7784..9133)
                     /locus_tag="ACHK8E_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP0346572.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9179..9937)
                     /locus_tag="ACHK8E_004390"
     CDS             complement(9179..9937)
                     /locus_tag="ACHK8E_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346573.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9969..10682)
                     /gene="yghS"
                     /locus_tag="ACHK8E_004391"
     CDS             complement(9969..10682)
                     /gene="yghS"
                     /locus_tag="ACHK8E_004391"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0346574.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10856..11548
                     /gene="yghT"
                     /locus_tag="ACHK8E_004392"
     CDS             10856..11548
                     /gene="yghT"
                     /locus_tag="ACHK8E_004392"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0346575.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11597..13096)
                     /gene="pitB"
                     /locus_tag="ACHK8E_004393"
     CDS             complement(11597..13096)
                     /gene="pitB"
                     /locus_tag="ACHK8E_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP0346576.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            13033..>13216
                     /locus_tag="ACHK8E_004394"
     CDS             13033..>13216
                     /locus_tag="ACHK8E_004394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346577.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYLFLTIKRVLFLVYQ"
BASE COUNT         3351 a         3425 c         3257 g         3183 t
ORIGIN      
        1 aaaatactat ctcatttatc ttttatgcct atataatcca ccgcattatt gtcattttat
       61 tttggatatc caattttttc gtggtgtata tgtctaataa aatctttacg cattccctac
      121 ctatgcgcta tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc gctctgagta
      181 gcgccggaat gaatttttat gacagacgtt gccaacttga agatcaactg gaatatcaga
      241 cgttaaaagc gcgttccgaa gctggtgcga aaaggttgtt atcgctgaac ctgaaaaaag
      301 gcgatcgcgt ggcgctgatt gccgaaacga gtagcgggtt tgtagaggct ttttttgcct
      361 gccagtatgc cggcttagtc gccgtcccgt tggcgattcc aatgggcgtc ggtcagcggg
      421 attcctggag cgccaaactg cagggtttac tggcaagttg ccagcccgca gccattatca
      481 ctggtgatga gtggttgcca ctggtcaatg ccgcgacgca taacaacaac cccgaattac
      541 atgttttaag ccacgcctgg tttaaggcat taccggaagc cgatgttgcg ctccagcgtc
      601 cagttccaaa cgatatcgcc tacctccagt acacctccgg cagcacccgt tttccccgtg
      661 gcgtcattat cacccatcac gaagtgatgg ctaatctacg tgctataagc cacgatggca
      721 ttaaattacg ccctggcgac cgctgcgtct cctggctgcc tttctaccat gatatgggac
      781 tggtcggctt tctcctgacc cccgtcgcca cgcagctttc agtagattat ttgcgcactc
      841 aggattttgc catgcgtcct ctgcaatggc ttaaattgat cagtaaaaat cgcggcaccg
      901 tttccgttgc accgccgttt ggctatgaat tgtgccagcg ccgcgtgaat gaaaaagatc
      961 tcgctgaact ggatctttcc tgctggcgcg tcgctggtat tggagcagaa ccgatctccg
     1021 cagaacaact ccatcaattc gctgaatgtt ttcgtcaggt taactttgac gataaaactt
     1081 tcatgccgtg ctacggactg gcagaaaatg cgctggctgt cagcttctct gatgaagcct
     1141 gcggggttgt ggttaacgaa gtggatcgcg acatcctcga ataccagggt aaagccgtcg
     1201 cgccgggtgc tgaaacacgc gccgtatcga ctttcgtcaa ctgcggtaaa gcgttgccgg
     1261 aacatggcat tgaaatccgc aatgaagcag atatacctgt cgcggaacgt gtggtaggcc
     1321 atatttgcat ctccggcccc agcctgatga gcgggtactt tggcgaccag gtttcgcaag
     1381 acgagattgc cgcgacgggc tggttagaca ccggcgacct tggttatctg ctggacggtt
     1441 atctgtatgt caccggacgc attaaagatc tgattattat tcgtggccgt aatatctggc
     1501 cgcaggatat tgaatacatt gcggaacagg aaccggaaat tcattctggc gatgcgattg
     1561 cttttgttac cgcccaggaa aaaatcattt tgcagatcca gtgtcggatc agcgacgaag
     1621 aacgtcgcgg gcagcttatc cacgcgctgg cagctcggat ccaaagcgaa tttggcgtta
     1681 ccgcggatat cgatctgttg ccgccccaca gtattccccg aacatcctcc ggcaagcctg
     1741 cccgtgcgga agcgaaaaaa cgttatcaga aggcttatgc tgccagtctt catgtgcagg
     1801 aatccctggc atgaatcaaa ctgtcgcggt gacgggcgca accgggttta tcggtaaata
     1861 tattattgat aacctgctcg cccgcggctt tcacgttcgc gcattaacgc gtactgcccg
     1921 cgctcacgtc aatgataatc tcatctgggt gcgcggttcg ctggaagata cacattcgct
     1981 tagcgaactg gttgccggag ccagcgcggt ggtccattgc gccggacaag tgcgcggaca
     2041 caaagaagag atttttaccc gctgtaacgt tgacggcagc ctgcgcctga tgcaagcggc
     2101 aaaagagagc ggcttttgcc aacgttttct gtttatctct tcgctggcgg cgcgccatcc
     2161 cgaactctcc tggtacgcaa attccaaaca cgtcgccgaa caacgactga tcgcaatggc
     2221 tgacgaaatt acgctgggca tttttcgccc gacagccgtg tatggtcccg gcgataaaga
     2281 gttaaaaccg ctgtttgact ggatgctgcg cggcctgctg ccacgacttg gtgcgccaga
     2341 tacacaactc tctttcctgc acgtcaccga ttttgcgcaa gcagtgggtc agtggttaaa
     2401 cgccgaaact gtacagacgc aaacctatga attatgcgat ggcgtcgctg gcggctatga
     2461 ctggcaacgc gtacgacagc ttgccgccga agcccgttgt ggttctgtgc ggatggttgg
     2521 cattcctctg ccgctactca cctgccttgc ggatatcagt actgcgttga gtcgcctggc
     2581 aggtaaagaa cctatgctga cccgctcgaa aattcgtgaa ttaacccacg ccgactggtc
     2641 ggcaaataat aaccgtattt ctgaagatat taattggttt cccgggatta gcctggaaca
     2701 cgcattacgc aacgggctat tttgattgtc ttgtttaaag gtgtgttaac gtttctatgg
     2761 taaatcgtga aatagtaatg gattacatcc tttcctgttt acaggatttg gtcgaaaacg
     2821 gtgtggaaat taagcccgac agcgatctcg tcaacgatct tggcctggag tcgatcaaag
     2881 tgatggatct cttaatgatg ctggaagaca gattcgatat ttccattcct attaatattc
     2941 tgctggatgt caaaacccct gcgcaattaa tggaaactct acttccatgg ctggagaata
     3001 aataatgggg ctatacgata aatatgcgcg ccttgctggc gagcgcctgc aatttagtga
     3061 caacgggtta acaccatttg gcacctgtat tgatgaagtg tattccgcca ccgaaggccg
     3121 gatcggcaat aaaaaagtga ttctggccgg gaccaataat tatctcggtt taacctttaa
     3181 tcatgatgct atccgcgaag gtcaggcagc gttagcggcc caaggcactg gcaccaccgg
     3241 ttctcgtatg gcaaacggca gttatgcgcc gcatctggcg ttagagaaag agattgccga
     3301 gtttttcaat cgccctaccg ctatcgtttt ttcaaccagt tataccgcta atttaggtgt
     3361 catcagcgca cttgccgacc ataacgccgt ggtgttactg gatgccgaca gccacgccag
     3421 tatttatgac gcctgttctc tcggcggcgc agagattatt cgtttccgcc ataatgacgt
     3481 caacgatctg gaacggcgca tggtccgcct cggtgaacgg gcgaaagagg cgattattat
     3541 cgttgaaggc atctacagta tgctcggtga cgttgcgccg ctggcagaaa ttgttgatat
     3601 caaacgtcgt ctcggtggtt atttgattgt cgatgaagca cattcgtttg gcgtgttagg
     3661 cgcaacaggc cgcggactgg ttgaagccgt tggcgttgaa gacgacgtgg atattatcgt
     3721 cggcaccttc agtaaaagtt tggcttccat tggtggtttt gccgtgggtt cagaagcgat
     3781 ggaagtgctg cgctacggca gccgcccgta tatttttacc gcctctcctt ctccgtcctg
     3841 cattgcgacc gtgcgctcgt cgttaagaac catttccaca caaccagagc tgcggcaaaa
     3901 actgatggat aacgccaaca atctgtatga cggtctgcaa aaactgggtt atgagttaag
     3961 ttcacatatt agcccggtag tgccagtaat aatcggttcg aaagaggaag gtttacgcat
     4021 ctggcgcgag cttatttcgc tgggcgttta tgtgaatctt atcctgccac ctgccgcgcc
     4081 tgcaggcatc acgctgctgc gctgtagcgt aaacgctgcc cacagtcgtg agcaaatcga
     4141 tgccatcatt caggcatttg ctactctgaa gcagtaatgt gagcccgctg tggtgttgcg
     4201 ccacggcggg tttaatgttg tctgtcatac caatacacca ggctcaacgc caccagcaca
     4261 aacgctaccg gtaaaccgag cgcggtaaca ggtgcgctca acgcaaataa caatcccaga
     4321 ttgaccatga tttgatagct gatccaggtg agtaagccaa cgatgacgcc tacagcgaga
     4381 cggctaccca tcccaggcga acgcggggcg ctaaaggtaa acggcacagc cagcaaaatc
     4441 atcgccaggg tcaagatggg ttgtcccagt ttttcccaca gtgcaaggcg gtattcgctg
     4501 ctgggttgcc cggtttcctg caaataatgg atgtaatgat taagttgacg gacagaaaaa
     4561 gtatttcccg gcatcgataa ctcttcaaga ttcatactgg tgaaggccga ttgccaggga
     4621 agattatcag atgtttcctg cgtttctttg ccattcagcc attttttatg attcacacca
     4681 tgcaggatcc acgttttgtc gtcttcaatg gtagcactgc gcgcataaat gtaggattcg
     4741 agcgagagat catcgcggta atgaaatatc tccacgtcca ccggctggcc ctgctcgttc
     4801 agcgatttca ccgtcacaaa ttcattacca cgcctggccc acagcatatt gccggtaatg
     4861 tcatcgtcct cccccaacgc agtggcagtc gatttgattt gcagcgcctg ttgctgcaat
     4921 ggcgacgcca cccactcatc tatcgcgcct aatgaaacag tccacaatat cccggcaacc
     4981 agcgccacca gtgcaatacg gaagatagaa aaccccgtgc tgcgaatggc ggtaagctca
     5041 ctgtttttcg ataactgccc caggccgaca atccctccca ataaggcgat aaacggcgaa
     5101 agttcgacca gtgtgcgcgg taaggtcatc agcaccacca gcaccgcctg agtccagcga
     5161 taaccacccg ggctgacatc atccagttcg ttaatcaggt tgaaggtggt aaaaagcggc
     5221 agcaatagcc ctgcggcggc ggcaaaaccg agaaagagat gacggattaa atagcgactg
     5281 aaaacattca tcgggaaaat ttctgcagca aagagaagtc gcgtgcgacc aacatcagca
     5341 ggcccattag catgagtccg ggtactaacc acaaaccagg aatgagggga atcgcgccgt
     5401 tagccaccag cgtacggcag acgtcgccgc cataaaaaat ggcaacaaac agcaacgtca
     5461 gtggcaataa cgttgaaaat cgcccttgcc gcggttttac ccgacttaat gaaatggcca
     5521 gcagcgccat caacaatgct gtcaggccac ggctttgtcg ccattgcagt tcggcatgat
     5581 cggcaggaaa ggcggagcgc gccagctccg tgaccgattt tgctttgcgt ttgacgttag
     5641 ggctttgatc cagcggattc agatgtaatt gcagattacg gtagatctgt tcgttgtcgt
     5701 cacgcccctg atgatcgaga agataggcgg tcccgttatg caacataacg gtcggtttct
     5761 caggtgatgg gtcaaccaca tcaaccgaac gggcgcggaa aatgcgggtt cgattagcag
     5821 tagaagtata aatcagcgca tcagtcaggc gattattatc ctgatcaacc gtctgcgaaa
     5881 ggatcattcg tccgttatcg ttagtgttaa atttctttgc ccgcaactgg cgaacatcca
     5941 gctccgactg tgactgttgc tccagttgat aaatctgcgc ataagcccac ggacgaccat
     6001 acatcgacag aagggtgaca aaaatactta gcggtatcgc cagataaaga acggctttgt
     6061 acaagcgtcc cggactgccc cccgccgcag agatagcggt aatttccgag tcggtataca
     6121 tctgccctag cgtcacgcca actgacacat acagtccaac aggtaacaac atctccagtg
     6181 caatcagcac tttgtagaaa acgatatcca gcacaacatc aagagctaat gtcccatttg
     6241 ccgcttcggt cagataacgc tgtgcggagt agctggcgaa aataaagatc agaaaaccga
     6301 cgataatcag caccagacgg cgcgtaccac gcatgatgta gtgttcaacc agcttcatct
     6361 cactaacgcc ctcaatcctt tgaattttta ttcattgatc agcttctgcg ccagcgcaat
     6421 gacatgatct acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggt
     6481 tttattcagc accagaatgg ttgaactctg ctcctgcaag tgccaggcaa cgcgtccctg
     6541 tccattgccg tcaataacga attgcgccca tggatagcgg cgtttatttt tttcgatttt
     6601 gccaacgaca aaatagccgg taccaaagat ggcatcatca gtgttcacaa tggtggtggg
     6661 ctgaaaacga tcctgcggaa agtttgccgc ttcaactgct ttaattaaca gtgagttctt
     6721 tttcttcgcc gacttacgcc cggcgatata ctgaataatg cgaacttttc ccgccaattg
     6781 ggtgctgttc cacttttggt agtgaatttc gtcgtcgcta ttccgaagca gctcgccgcc
     6841 ttcctggata taaacggggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc
     6901 cggggctaag agcatgataa tcagcgcaat aatcaaacga gatgacatca ttgctccggt
     6961 tataccggaa aacgcgaaat cagcagttct aagcgctggc gaatatcgct atccagcgcc
     7021 atcggtgcat catacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta
     7081 tcccccatca ggccattaat ggcggcgagg aagcgggaaa tctgtaacgc ttctttattt
     7141 tcaggatcaa gcgatcttag ggtgtcgtaa taagaaacct gctggctgat gatttttcgc
     7201 acatcatgaa attccctgcc ggttagtcgc ggtttggcaa ttatctcttt cagggtatgc
     7261 atttgctttt gccgataggc aataaaaccg cttacgcttt ctaactggct atggcgtaat
     7321 gaatagttca ccaacgacca gaccggagag cttaaataaa cccgtaacag gttgccgtaa
     7381 tacgacacaa tatttttctt accgttacga aagccgtcct gaaaacgccc caaaaaaacg
     7441 gtcgttttgc cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa
     7501 cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc
     7561 tcatcgcctt tggcctgttt gcatattaaa cgcagtaact cctggcgatt gacgccattg
     7621 agccagaatt gcaggcataa cgcgtaatta tcatggatga tttcggtggt acaacttagt
     7681 gcgatagtcg gcggcaaact gacctgtgca tcaacgacat catcttcttc aatcgcaaca
     7741 aatagacgct cgctcgcctc tagcgggaaa tattttctca tcatcaggat ttctctccgg
     7801 ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt taaggtcgga attagcgaaa
     7861 gcaccacatc cagacattga ccaccgaaat aagcaaacgc tgctcccagc acgccaaaca
     7921 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
     7981 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgagggaag cccagcatcg
     8041 agatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
     8101 caaacaccag cgaaatgagc ggcttgccga caatcaacac cgcgagcgcc accagaatac
     8161 cgattccgcc cgccagtaaa cctgacttca cgcctaacaa ccacggtctg gtggtacgcg
     8221 gatctaaacg catcacctct gggtaaaaac ttttgcccag taaacccgct ggcgttccgg
     8281 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
     8341 tccccaccaa cactgtgctg cacgaattac gcgccgacca gatggaatgg gcaatgttgg
     8401 ttgaccagac aaaactccac gcgcctttga tgtgtcgggc agactcaaac agattcaatt
     8461 tgaacgcgtt atggatattt ctgcggcgta attcacgtgc ggcaaaccac cagtacatgg
     8521 tgccgccaac aagattcgag gcgtaccagg caataacaaa acccgcaaaa ccaaagtcaa
     8581 aataccaggc aacgacgctc cccgctgcgc gcagaaaagg tttcgtcgct tgctgtacag
     8641 caattagatc gaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
     8701 aaggaatgag cgtgcaatac agcgctgcca gccaaaagct ttggtcatcc agacctaatg
     8761 aatgggaaag aaaagggagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
     8821 tatccagcga gaaggaaaat gagacgacat tgcggaattg ctgcggatta ttgttggtta
     8881 atgctggtgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
     8941 tcgacttggc gtacgattgt acaatcacca gtacgccaaa catggcgggc gtcatccctt
     9001 taccggcaca cgagagcgcc aacagaccca gcaaagcgct cacgacatta ctggagccta
     9061 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
     9121 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaagtatcc cgccagcctt
     9181 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtgcctgtt
     9241 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
     9301 caatcgtcga catgacgccg attttgtcct gcagctcggc atagtcatgg tcaggcttgc
     9361 gggagatggc agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg
     9421 ccatttgttg gtataaacgc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
     9481 cacgttcgac gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga
     9541 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
     9601 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgct ggagatttgg
     9661 ttttcatgct ttgggttttc gaggatttgg ccgccagtcg ccgttcgagc cagacgccaa
     9721 ccaacggcaa tcgtttgatt ttgtcgccgt cttcgcccga gagcagcccc agatagcgcc
     9781 gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagt gtggatttac
     9841 cggtgccatc acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg
     9901 ttgtactatt aacagtttgt gtttgtagtg cgtccattcg ttaaatcatc ccctatccat
     9961 tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcag aatttcatcg
    10021 gcgggttgcc gaccatccag ttcaaggatc tttgcgccat taaatgtcag ttgcggcgta
    10081 acggcgattt tttcctgtaa cgccgccagt tggtggtcag gtttacgcgc aaacgcggtt
    10141 tgttcatcaa tgccaagacg aatcaacaat acgggcaaat aagacgccat ccattggtac
    10201 agcttcagct cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
    10261 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
    10321 agcagatagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
    10381 gaaagcagat agataactag cgcagtaata ttgccaggtg gtgtggaggg cttttcgtgc
    10441 acatgtgccg ctttacttcg cagataacgc ccaaaaggtg cgccaataac agggagctgt
    10501 gaaatccatt cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt
    10561 gttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
    10621 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
    10681 acgaaaaacc cggcagcaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
    10741 gttcaacctt attgtgacaa tggttaattg tgacattaac ctgaattacg ctttcataaa
    10801 aacatattaa tcaaataaat attttggatg gatatttaaa tttaaggata tattcatgca
    10861 gtcaataaca cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt
    10921 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
    10981 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta ccgttgatgg gaaagtctct
    11041 gcacaagact attgaaaaaa atgaagtgaa aacggcaaaa aaattgccgg gtccggttcc
    11101 ggccctggtg atcaccgctt tcgtcgcccg ccgactgtta cgctttcgtc atatgctcgc
    11161 ctgtcgtcgt cgcgggctga ttgtcttaac cgatcgttat cctcaggacc aaattcctgg
    11221 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggc ggccggtttg tctcatggct
    11281 ggcaagccag gaacgtaaag catttcactg gatggcgagc cataagcctg atctggtcat
    11341 caaactcaat gttgaccttg aagttgcctg cgcgcgtaaa cccgaccata aacgggaatc
    11401 gctggcgagg aagattgcca taacgccaca gttaaccttt ggcggtgcac aactggttga
    11461 tatcgacgcc aatcagccac tggaaaaggt gctggttgat gcagaaaaag cgattacgga
    11521 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
    11581 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacaaccca ccagaaagaa
    11641 aaatcgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
    11701 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
    11761 tggagacggg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
    11821 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
    11881 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagagctact gccatgatga
    11941 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
    12001 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcagagatgc
    12061 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
    12121 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
    12181 agtgaaactc cgttacttgc gtgccatcag tcgatgccgc aggcaatgga ggttccatcg
    12241 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtgacgg
    12301 catcgcgggt acgggtaatt tcatagccgg acgcattcat attgacgacg aaaccagcag
    12361 gggcaatccc caccagtacc agcatcacca ggccgatccc tttctgcccg tcgttagcac
    12421 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggtg
    12481 gcttacgttt gccttttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
    12541 ttttcgtccc gctccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc
    12601 cgacgatagg ggaaacaatc agcgaggaga aaattttggt cacttcacgc aggtttaacg
    12661 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
    12721 caatcaaggt gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga
    12781 taatcgcggc cagcagcatg gaaaagacca tcgccaggcc gtgagttgac cccatattca
    12841 gcaacaaatc ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcctagca
    12901 atacgccaaa aaagttaaaa aatgccgcca tcaccacggc aagctgtggc tgcatggctc
    12961 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
    13021 agaacaacac aaatgccaga gcaagtaata acaaaagccc tgtgtatata tcaaggccaa
    13081 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
    13141 agtgacttta acggcatggg caaagtgaaa tatctttttt taacgataaa aagagtgtta
    13201 tttcttgtgt atcaga
//
LOCUS       DAZGTJ010000116        12489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000116 DAZGTJ010000000
VERSION     DAZGTJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.116"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(148..450)
                     /locus_tag="ACHK8E_004395"
     CDS             complement(148..450)
                     /locus_tag="ACHK8E_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346578.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(797..1801)
                     /locus_tag="ACHK8E_004396"
     CDS             complement(797..1801)
                     /locus_tag="ACHK8E_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0346579.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(2059..2490)
                     /gene="tssE"
                     /locus_tag="ACHK8E_004397"
     CDS             complement(2059..2490)
                     /gene="tssE"
                     /locus_tag="ACHK8E_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0346580.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(2493..3029)
                     /gene="tssJ"
                     /locus_tag="ACHK8E_004398"
     CDS             complement(2493..3029)
                     /gene="tssJ"
                     /locus_tag="ACHK8E_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0346581.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(3010..4110)
                     /gene="tssG"
                     /locus_tag="ACHK8E_004399"
     CDS             complement(3010..4110)
                     /gene="tssG"
                     /locus_tag="ACHK8E_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0346582.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(4065..5828)
                     /gene="tssF"
                     /locus_tag="ACHK8E_004400"
     CDS             complement(4065..5828)
                     /gene="tssF"
                     /locus_tag="ACHK8E_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0346583.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(5962..7560)
                     /gene="tssA"
                     /locus_tag="ACHK8E_004401"
     CDS             complement(5962..7560)
                     /gene="tssA"
                     /locus_tag="ACHK8E_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0346584.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(7560..10949)
                     /locus_tag="ACHK8E_004402"
     CDS             complement(7560..10949)
                     /locus_tag="ACHK8E_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP0346585.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(10942..12090)
                     /locus_tag="ACHK8E_004403"
     CDS             complement(10942..12090)
                     /locus_tag="ACHK8E_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP0346586.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(12094..12360)
                     /locus_tag="ACHK8E_004404"
     CDS             complement(12094..12360)
                     /locus_tag="ACHK8E_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0346587.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(12391..>12489)
                     /locus_tag="ACHK8E_004405"
     CDS             complement(12391..>12489)
                     /locus_tag="ACHK8E_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013035836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP0346588.1"
                     /translation="MCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
BASE COUNT         3137 a         3526 c         2915 g         2911 t
ORIGIN      
        1 tttattttcc ttctcagtaa ctgaatatct ttgattaacg ccctccggaa atatttatac
       61 ccggaaggct gtccagcttt atgatacccg ccagtacaga attaactgca ggctgagcac
      121 agatttctct gctatttcag tgtcagatta atactgatta ccggaacaaa tgggcagatg
      181 tgtaaatcca ggtttgctgg ccatataaag cggcgcacag gtgggtttac ggggttcagt
      241 gaccggtaca acctcatttt tcccggccac cgacggttgt gacaccttcc ggacagccgc
      301 tgattttttc ttctgaacca gcttcgtggt gaccggtgat ttcacctgac gtttggcggg
      361 tgccacgcca taaccggaac aaaagcgctt cgactgactc agacttccgt cattgcagac
      421 aaaacgtccg tctgacgtgc agtgtgaaat accaccttta gcacccgagc agggttttct
      481 tccccgcgca gcatctgtct gaaccgaaaa taatctatgg tcgcccccgt ttttgcaaca
      541 ctgatttttt aattaatggt gggcttgctt aaatctatcc ggcgtctctg taggcaagga
      601 tgcctgcgcc ccgatgagtt tcgcacctga taatcctaaa aagcccctcg gcttcagtga
      661 gccattttat ttattaggtt tttaatcagg tcgatttgcc gttcatgtca tcaataatca
      721 gtcttcgcaa aaccagacag gcaactttgt atcttattgt ttacaataag ccaattgagt
      781 tatatcagca tttatatcaa gcctgatatt ctttttgctg agtagtgatt acccaggcaa
      841 ttcgagccag tttatttgcc agagcgcagg caaccacatt cgaatgcttc ttgttgagtt
      901 gctcccgaac ccattcggca agttttccat gctggcgttc aagccgcatc atgaatgagc
      961 gggcacactg aaccagtaac cggcgcaaat ttttatcgcc tcgcttactg atacccaaca
     1021 gagtcgattt tcctcccgtg ctatactgcc ggggaacgag ccctgtggaa gccgcaaaat
     1081 ctctgctgca tgaaaactgt ttcccgtcac caggttgcga cgatagtaag ctggcagtaa
     1141 tcggcccaac accaggaatt gtcataatgc gctgagcggt atcatcagcg ttgattgact
     1201 gacttaattc tgattccagc tctgctattt gctcaacaag gtaaaggtag tgggtatgca
     1261 acctcaccaa aaggcggctc agatattcgg ggatttcgtg ttcagccaac acgagagaca
     1321 accgttttat tacagcatca cctgtcggca ggctgattcc aaattccagc aagaaaccgt
     1381 ggatctggtt ggttgttttc accttatctc tgatgagtga ttctcggacc cggtgtaacg
     1441 ccctcatggc ctgcaggggc tcagttcttg gctggacaaa acgcattgat ggtcgtgaag
     1501 ccgcttcgca tatggcttct gcatcaacaa aatcattctt attacttttg acaaacgggc
     1561 gaacgaactg tggagaaatt agcttagttt catgcccaaa atcagagata cgacgagcca
     1621 taaagtgtgc acccgcacag gcttccatta caacaactga gggcggacat gtcgccagaa
     1681 actgcatcag tttagtacgg gtaaattttt tacgcagaag agccttacca aacttgtctt
     1741 gacaatgaat gtggaaggag tgctttccga gatcgatacc aataagtgtg atgttctgca
     1801 tgatggtctc tccgttaaag ggattcacct taacagcgta ctgctgttaa ggtgggggtg
     1861 accatctcat tagtcccacg aacagaaaac agataatttt cttcatttaa aaactcatac
     1921 attgttattg ctgatatgcc agagttatca ggatgatgaa acattccgcc agcgttgtca
     1981 taagccggaa taatgtcaca ttcacacctg acatatatcc atcactgttt atttctgttc
     2041 tgccctccct gctccggctc acgtctgaac gtaccgctgc tgtttcagat ggcgcaacaa
     2101 caccctgccc tccggtataa atgttgttcc gtaacgaacc agcccggcat ctttaagttc
     2161 tgcatcaatg acataatgca actcaccagg ctgagtctgc tcctgcatcg tcacgttgac
     2221 acgcttaata cgtggctcat acttcagcag aacatctgaa agcacacgca tcagttgatg
     2281 cgccgttccc ggcattccct gcagaacggt tgtcatatcc ggcagaccat aatccggcag
     2341 atgtttaagg cttccggccc gggtattcag aatacgctgc atgttatcga gcaccgaaag
     2401 aataacctgc tcttccgcac tgacctggtt taactccagc ccgccagcga aattgccata
     2461 gagaatttca taaagggaag gacgcggcat cactatttat ccttcaccgg taacagcgtc
     2521 agggtattac cggatacttc aataagacgg ggcgtgtccg gctcaagctc atcccggctc
     2581 agcaccaccc gccaggtatt tttcttccgg tcaggctcca gaaacattgc tgccacaccg
     2641 gtaaatttcg ccgcatcatc cagaggcata tccacggtta ctgagccacc tggctgcatc
     2701 catacatctt tctgtgcaat gatatcgcct gccagaattt cactgtcccc cgtaaaaagc
     2761 gcctgataat ccgcgctgtc aaaattccgg ttgtctttaa gctgataaat ccgcaccaca
     2821 accgacaggg gaataccggc agcactggtg ttaatggcct ccctggcacg aatatcaagg
     2881 tgaagtgttt ttatctgtcg gtaaaacagg gatttcgtgg cagataccgt accgtctgcc
     2941 actctttgcg tcaggccaca ccctgataac gacagggaaa acaacgcaat aatcagaccg
     3001 taaccagcct taccagcgat aatcgccatt ttcctgagcc tccttacgtt gataatgttc
     3061 ctgaacccgc tgataacgcc ccagacggat ggtcattacg cgtgccgctg aagtcgttat
     3121 ctttgcctgt gttctcatta cagccgtgcg ccccagttgc ggactaccgg ctgccggtcg
     3181 gcaactgagc cggacatccg gcaacagtga acgctcaaca caaagttgca gacgtacatc
     3241 cagacggctt cccagataaa cgtgcaacaa cgccagcaaa tcagaataca gctgcccgcc
     3301 cggtaaccag ccctgtacct cgctgcccgt ctgtgttgaa agctgtaata acacctgtcc
     3361 gttcacatcc gtagcatgat cgcccatcac cgggcgactc ttcagactga ccggatgatg
     3421 aacacgcatg gccagcggtg ttttcagcgg gatccgtcgt cggtcatgat gccagacacg
     3481 ggcctgtgtt ccgggagcca gcagcgttac cagtgatgtc agcccttcag ctgttcttcc
     3541 gggtaacaac atcaggggca ataacgccag aaaacgcgat accggcgtgg caatattctg
     3601 tgcacatccg gggatcccca gtctggccag accgagcaga tactgggatg ttttatcctg
     3661 cccccccgcc tcaaaggtgg ccgggtacga atatttgcgc cagatacggt aatactgggt
     3721 gatcagacga tgactgaata tatcgagaaa atccgctgcg gcttcatggc cttcccgctg
     3781 ctgtgcaata tcactgatat agtgagccgg caacggagac gtgaccccgt aaagtcccat
     3841 aaacgtcaca tgtacagtgg gtggcagatg ggattcttca ggaattacag catcttttat
     3901 ttcactcgca ggaaacccca ttcccgtgta tggacaaaag cgaaccgctt cctgacgggc
     3961 ctgccagcca ctgccaatgg gaggctgatc tggctggctt tgttccagta actgacagaa
     4021 gcgataaaaa ttaatatacg gaagctgttt ccggtaacgg gtaattaacc gggcaggcgc
     4081 ggattgtgat tttctttcca ccggatgcat tttccttcag gctgaacaat cagtgtgagc
     4141 tgattgaact gattcatatc ggcgtacagg gccaggaaac ggttgagcat ttcgccaaac
     4201 aggtgaatat ctccctctcc ggcaaacccg ttgccatcaa gagttacctc cacatcaagc
     4261 ccccggagca gaaaaccctt ttcaaaacgc tgaatacgat gatgctgtac agccagaatg
     4321 gcatcaagcc gacggtgatt aagttcatcg tcccgccagt tataaagcgc cagcgtgcca
     4381 cgcagcactt ccgcactgct cagcatgtta agaaatcctg tccccagatg actcatcacc
     4441 cgccagtgaa aacgatcttc tgtcggcgga taaaccggca gcgtgggttt acaaagatta
     4501 catacactga tcggggtctg cagtacctgt tcacaccgat caagcagcgt actctgcaac
     4561 gcacgacggg gaagctgacc attggtacct gtaatacgta atgaaagcgt ttcccgctcc
     4621 ggcatccgat ccgcctccca ccgctgaccg ccaaggataa gccatgtgtc atacatcccc
     4681 gtgacacccc gcttcacccg ggtatgataa taacgttcgg gtgcatcatg gcgcagcatt
     4741 cccccacgat gacggaagct ggtaaaaggc acgtactctg cactgccggt tcgcccggag
     4801 cccgccaccg catccactga atatatctcc gtatatccat cctgcagacg cttcgggcgc
     4861 agcagatatt cgctctccag cccattgatg atgagtggat cggcttcaag cgtaaaaagg
     4921 ttaatcaccg gcacacaatg cagacacagg gcatcatctg tcacaggtaa atctgccggc
     4981 caggactgac tcagcaccac ctcaatatca aatccggaaa tacctgccgg caaagaaata
     5041 ttctccagtc cgttcagatg aacaaacata aatttttccc gaaaggtgaa atattccagc
     5101 aataactgat acccactgaa agcactgtca cctttcggcc acagccgatc ttcttccgca
     5161 aaaccaccgg gtgaaaacca tccgtcaatg cggatccgct catcatttcc cggcagcctc
     5221 agatacagcg cagccagacg ctttgtcatc atcagatgaa gggtgctgct gaccggggct
     5281 tctgctgcca gatacaggct cagacgatgc aaatcgacct gagaccagtc agccagttca
     5341 ctgcaggcaa aacctatttt cagcactgag cgcccgtccg gctccgtagt cataacgacc
     5401 ttctccactg ccagtggatt cagtggtatg gcccgggtgg tacggtaacg acagactgta
     5461 tttccggggc caacagggcg ggaagtcact tccagtccgg ctggtacggt ttcagccatt
     5521 ttcatgacgg ataaccgggg agtcagtgcc accaccgaca gggacgggat agtacgcagg
     5581 taatgaggcc agagcatact gaccagactt tcagtaagct ccggcaaatc atcatcgatt
     5641 ttctggcgca gccgtcccat ggaaaaggcg aaaccttcaa acaggcgttc cacgcaggga
     5701 tcaggcgtac ccgctttatc gagatccagc atcgccgccc gatcgggatg agcctgtgca
     5761 aacgcctttc cggcttcacg cagataacgc atttcagcgt caaaataacg cagggttaaa
     5821 tcgtccatta tcttctcaga ataaagctaa ctgttccgga gactcgcgct ctgacttcag
     5881 ccatatttca aacaatcgcc cgaactgaac tgcaggcagg atttcattaa tcagattaca
     5941 catgaatata attcagacat ctcatgaact tcccgaattg cacaacacag cagctctggc
     6001 agcatcaatg gcgatgagcc cggcaagaag atgttccata tcaggctgca gacgcaccct
     6061 gtcactttca gtttttgcgg acttcatacg taacagtttc aggcggcggg cttttacctc
     6121 aaataccagt tcaggttccc attgtgacag ggtcagccgg gttgcacgtt catccagctc
     6181 cacaagcaga tgcagggcaa ggtcattttt tccagtctgt tcggcaacac gggccatcaa
     6241 caggcgcagt agccagttgc tgcggtcaga ctgaataccg ggacgtgcct gtaaccagct
     6301 taaagcggct tctgttcctt cattatccgc aatctgcagg gcttccggct caagagataa
     6361 aatgtcgtta tctccgggag ctgccgttat cgttacagaa ggttcttccg cccagcgttc
     6421 accacactcc atcacctgct gctgtatcca gctcagcgtc acgtcatcgg caaacggcat
     6481 gccgtcgtta aaagccagtg tctcaagccc cggcaggcgc agaagcaatc ctttcaggtc
     6541 atactgaata atggcagcat aattttcttt tccggtctgc aacagcgcct gatgaatata
     6601 ccactgcagg tccagccaca gatgactggc tccccgggca aacatatcgt cacactgttc
     6661 aagcagtgac aaccagttct gctgcagata caaccgtttc agtgacgcac gccggtccgg
     6721 ccccggtggg gcaatacggg tccgtccatc agcagataac ggcggtaact gatgcagcgt
     6781 gtcatgccgg acgcttttca tcagtcgatg agccgccagc cagccctcag gctggtcacg
     6841 cagatatttt gccagaacac gggcctgtga cagcagttcc tgtccggagg tgatggcaga
     6901 taatgccatg acgctgccgg atgttaccgt cggtgccggc gcggcagcct cctgaggcac
     6961 cactgcatca acaccaccgg ccttcatcag gcgattttca agtgcctgat ataacccgtt
     7021 cagcaccggg gcggaagcct catccgtaaa ggtctgttct gccagccaca gcgccccggc
     7081 aatgcgcagg gtatccgcct tcaccacctc cggatacaga gacagactgt ccagtatgcg
     7141 ggcgctgcat aaccactcca gcgccgcctt cctggcccgt gaccgttgtg gatgcaggtg
     7201 ctctccaaaa cgctgcaaca aaccggtcag cagctccaga ccgtcggcca gccctttttc
     7261 accatcctgc cgcagacggg cccagatata ccaggtcgcc acccggatat ttttggttct
     7321 ggtggtcagt aatttttctg caagctggct gacaattccg gcatcggctc cggagagttt
     7381 attgacctct tcacgcatct gctgaaaatc atcatcgtac cccgcatcat cacctgcggg
     7441 attatctggc gtcacgggag caagccattt atcccacagc gcaatacgca cctgagccgg
     7501 ttcagccaca tcccggggtg tcgtctggca tgcactgagg agcgaatgaa tggaagccat
     7561 cagtcagtct cctccacgga atccccggca tcttcgtcat tgcctgtaaa cgctgacgta
     7621 ccggagagtt cgaaaaccgt ttccggcaga acaaaattgc ggagtttcag caatacaagc
     7681 ggcccttccc ccgcttcagt tcgcagtgtg taattcagca tacgaccgtc ctgcgcctgc
     7741 cagctcaggc tccagccact ggcaacgccc ggtgcggctt tccgtcgggc catctcaagc
     7801 agacgtatca gcccccagct tcccggcagg tctgcatact gacgggtacc ggcctgagtg
     7861 cttacccagc ttaaactggc gccaggtgct tccgtatcag ccggccaggt aaatcgcttc
     7921 cataccggca tctgattaac ataaatcagt ttctgattat cggttatcag cgtcgtctgc
     7981 atcacaccgg cagcagttcc cgggcgcagc tcaaaatgca gccccgcgtt acctgtggtg
     8041 aacgccacat ccgctatttc actcagggta ttgatggctt tcagaaacgc agggttgaat
     8101 gtcagtcccc gggtattaat ggtatcaggt acccagcgac tgccttcact gtgcaacaca
     8161 ccactcaggt tattctgcag gaaacgggca atacgtcccg tgtccgtatt caggtatttt
     8221 gccagcaggg gaagcgaagc atcgctgctg acatttttaa acggataacg cccggaaaat
     8281 gcattattcc agccatccac caccgcagta cgccaccggg cattcagact ttctgccgcg
     8341 ggcgtaagaa cctgctgcca cgcctgctcc atcgggcgaa caaagaccgt ctgtccgaag
     8401 ccataccact cctgtccgag tccggcagcg gtcaggctac cgtaatcccg ggtatcggtt
     8461 aaatcaaccg atttcccctg caatactgtc tgtgccagca tctgcatcat ggcctgcggg
     8521 tcagaagagc cggcaatctg ttgcagacgc agacgaacct gtgtcacccg ggtcagatat
     8581 gtctgcagat tcagcatatc cgcactgttg ttctgattat ccatcagtgc aagcaccggc
     8641 ccgaaagtcg tcgccagagg accgtgaagc cggctttccg gtactgccac tggctgtttt
     8701 tcctgtgaaa gcaggtttct ggcagattta accagtgaat cagtaaccgc ttcccggggc
     8761 tggccagtac gcccctgaac cgccagcgta ttcatcagag caaccagggg ggactggcgc
     8821 acatcagcca tcagcgtcag ttgctctgtc acatcagaca gcgtctgagc tttacgcaag
     8881 tgcagactgt tcagaaaatt cagccaggca ttaccaaaat cagcaaaata gcgcgttgtc
     8941 aggcgctgtc tgagtgcttc cggcgatacc ggtgacggtg ccttctgcct gccatccgtc
     9001 agtacccagt ccatttcttc acgacgctca ttaatcacgg tgtctatcga cggcaatacg
     9061 gcctcttccc aggcctggcg ggtaaacata ccgggaacca cttcatccgt aaaaaagagg
     9121 cgggagacat cagtatcacc ggtcatatcc gtcagacgca tatccgcaaa ctgatgtgcc
     9181 acccgtgcca gcattttctg atacagcatg gcatcgctgt tctgtgttcc cagatggcga
     9241 agcagcagag ttctgctctg gctgaccagc tcttcatcag ccgtcagctt ccactgagga
     9301 tgagtaataa tcccggaggc ataatacgcc agcaacgtcg ggccattagc ctgccagaaa
     9361 acggcactca caccgtcacg ctgtggccat tcccgcatga gcgtacgact gaacctggcg
     9421 ggctccatat gttgcggttg cgccagcatc agatacagtc tgagctggtc atatgcggct
     9481 tttgccattt tcccccgctg cgggctgtcc ggaggaagac gaataaatgt cctgagctgc
     9541 tgctcaagat gagcagcagc ggcatcacgc agtaaaagat gtgcattttg tgcataaagg
     9601 ggcattacga ccgcgagcag gtctgcattc tgattcatac cggcccgcag gtaccatggc
     9661 gccccgtgct cctcccggta ctgaaggcgg gccagggatt tctgcagatt cagcaacgcc
     9721 tgcagacgtt ctgcaggaga cagttgctgg ttctgagccg gacgaatctg ttcctgtact
     9781 tcagcaacca gactccggtt tgccagaaag gaaaccacca ttcctgttcc ctgagccatc
     9841 atcagtatgg ccgccataac ggccagactt ctgcgccagt tatgacctgt gcgggaaggc
     9901 tgtagccgga caggtaattg ctgcactgtc tccggcaacg cctcccagcg gttatccatt
     9961 cgccagcgat gtctgacact tcgttcaccg ccgacagttg ccggactgaa aacaataccc
    10021 gccagcaaca actgacggta tggacggagt aatacggata atggctccac cacagcatca
    10081 atatttcgtc tgaatcgctc tgccagcgac aacaaaaaat aataccgagg ggcacagcat
    10141 atctgctgta tcccctgagc aacaagcccg gggagcatgg cctgtagttg tgctttcagt
    10201 ttgtcagaac tacattctgc tggcagaagg catcccacgg gctgagtaat acgccctcgc
    10261 tcatccggac tctcctgcag tgaccagaca tacagcggca gacgccagcc aaggcgctca
    10321 caacatgaac tgaccgcacg ggaaaccctg tcaagggcat cttctgtcag tggtgcggat
    10381 aatgtttcac tcagtccgga agttacccag acaatgccat cagcaggccg gtaacgcaga
    10441 cggcgtaacg cagccagcca gtctgcgttt tccggctgcg aggggtctcc tccccagaga
    10501 agcaatgtcc cctgctcttc ctgccagaac tgttctgtca ggcccggggt cagcaactca
    10561 acctcagagg cagtacctgt tatcaacaga atgcgtattt tacgtcccca gaatctgcca
    10621 tgctggcgcc gcagagtatg acgaatatcc tgaacagtaa catagcgcgg tgacgtctga
    10681 gccagtcgct cttcatcgtc aggtaacctt tgctccttac gggcccgacg tcgatatgtc
    10741 agttcctgaa catatttttt catcacaggc aggtaacaga caaagagcaa ccccagactc
    10801 agcaatatca gcagtagtat ttgatcccgg cgtgtttctg cccccagaaa tgctccatat
    10861 cgggttatca gccaccagag cgcagccaca ccaataaata caatccccgg tcgtccaaag
    10921 cgaccagaca gacaggccag tttattcatt ctggtttcct gatgtaacag tactgataaa
    10981 atgttcttct ccggatgaga ataaagtcag gagagaatca ttaccaaaac agaccagatc
    11041 ggctgccaga gcagtaagca gccacggagc agcaggtccc gggataccgc accatttttc
    11101 cagtaacata cgtccggacg gttcccatcc ggcaccatac tggtttccaa tagtcatcag
    11161 cggtgcagca atcttttccc agtgataaca gtcccccagt acacgagcgg tacgacaggc
    11221 agctgtctgg gtttcaagga aaagtgtgaa ttcatcatta aacttgttga tatccagcga
    11281 catcgggcgt agcagacggg ctggatgcgg caggttatat ttttgcgcaa catcatcgct
    11341 ggtcaacaaa agtgcagcca gtccgtcaga gtatgcattt cccccattaa gctgtattac
    11401 cagtatcaaa tccacagtca gcactggttg tttcagacgc tcatcaaccc accccatgga
    11461 aaagtcggga gtaacctcga tattgtcagg aacaacacgt tggggaaata acacttccca
    11521 tgcagaaaca aaagcagatc tgatttcaga atcaggctga tcggttatca gcgtcacatt
    11581 tacagggaga acggcaggta actgacaaaa tttatccatt atctccctaa gcaagacacg
    11641 taaagaggct tcaaccttat gcccactgtc agaaagataa tctattttct ttacaaggcc
    11701 gtactgcaga ggcaattcat cacaaagagt agctaccgtt attttatcag gcaaataaac
    11761 acaactggca gtaataacaa gatacctttc ggcccaggat tcccactgtt tctgagcata
    11821 ctccccctct tttttaaaga acaaatgttc atccatctgg ttgctccact gccagtgtcg
    11881 caggcaaaac aggaaaaacc agccccccgg aggagtcaat gcaagccacc agatactgaa
    11941 atcatttaat gctttcacat attctgatgc ccggataata aacaacagag caccaacaac
    12001 agccataaat gcccctgtca taaaccacaa catcattgat ggtttatgag gtaattctgt
    12061 tgataatgat tttgttctgc tccatcccat gggttatcct attgcacatt caggtgcact
    12121 ggatataacc ttacacccac acagacattt acatccatca atcacaacag ctcttccatc
    12181 agatatccat tcaggagagc cctccacaat aggattagta ccatgtccgg aaacaggaca
    12241 actaaccata tctccgacaa gagctacttt tttaccatcg acaatcatgg ttgaagatgc
    12301 cgaaataact ttaccaccat gtgtggtatt atcaccaagc aatacaaaac ctttagacat
    12361 aaaactctca aaaataaatt ttactttgag tcaccagtta ccgtaggggt aaattttccc
    12421 tttgataaaa tctggtgtat caccgtaata aacatatcca tctggatgtt tagtaacccc
    12481 cttacacat
//
LOCUS       DAZGTJ010000117        12447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000117 DAZGTJ010000000
VERSION     DAZGTJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.117"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..280
                     /locus_tag="ACHK8E_004406"
     CDS             <1..280
                     /locus_tag="ACHK8E_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFP0346589.1"
                     /translation="VSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISES
                     GEVVDMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            280..5550
                     /gene="traI"
                     /locus_tag="ACHK8E_004407"
     CDS             280..5550
                     /gene="traI"
                     /locus_tag="ACHK8E_004407"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP0346590.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMTFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PVTIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLESGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSASVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLSTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGSGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGLEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPCAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAQEKPDQPDDKTEQVVRAIAGQEQDRASSSERETALPESVLREPQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5570..6316
                     /gene="traX"
                     /locus_tag="ACHK8E_004408"
     CDS             5570..6316
                     /gene="traX"
                     /locus_tag="ACHK8E_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFP0346591.1"
                     /translation="MTTDNTNTTRNDSLAARTGTRLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNQSRHAHIRQSAINRLWGWAVIAQF
                     AYYVAGFPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVG
                     IILLMFCHRIYRTTHLSVRSMLALCLFPVVALVNLQDSVTAAVSGVLVTYMVVRMVSG
                     IRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            6399..6767
                     /locus_tag="ACHK8E_004409"
     CDS             6399..6767
                     /locus_tag="ACHK8E_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFP0346592.1"
                     /translation="MKKILLVAAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGMT
                     EGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEVD
                     ISDEGNRKATVDVLTLERLR"
     gene            6803..7360
                     /locus_tag="ACHK8E_004410"
     CDS             6803..7360
                     /locus_tag="ACHK8E_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0346593.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            7512..7715
                     /locus_tag="ACHK8E_004411"
     CDS             7512..7715
                     /locus_tag="ACHK8E_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346594.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            8403..10397
                     /gene="cirA"
                     /locus_tag="ACHK8E_004412"
     CDS             8403..10397
                     /gene="cirA"
                     /locus_tag="ACHK8E_004412"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP0346595.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSAGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
     gene            10820..11281
                     /locus_tag="ACHK8E_004413"
                     /pseudo
     CDS             10820..11281
                     /locus_tag="ACHK8E_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            11647..12030
                     /locus_tag="ACHK8E_004414"
     CDS             11647..12030
                     /locus_tag="ACHK8E_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346596.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     RQVYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVVVREQDMKKMEAEFGRIIKIH"
     gene            12310..>12447
                     /locus_tag="ACHK8E_004415"
     CDS             12310..>12447
                     /locus_tag="ACHK8E_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0346597.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YE"
BASE COUNT         3213 a         2752 c         3722 g         2760 t
ORIGIN      
        1 ggtgtctcct gccatcaacg ataagaagtc agatgcaggt gtgaatgttc cggcaggggg
       61 gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac tgccacccgg
      121 gatcagtgaa tccggtgaag tggtggatat ggccgtttat gaggcatggc aacgggagca
      181 gaatccggat atccagcagc agatgcagcg ccgtgaagag gtgaatatca acgtgcaccg
      241 ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt cagcgttgtt
      301 aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta tgtgctgggt
      361 agcatggggg aacgctgggc gggcaagggg gctgaacagc ttgggctgca gggcagtgtt
      421 gataaggatg tctttacccg tcttctggag gggaagctgc cggacggagc ggatttaagc
      481 cgtatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt ctccgccccc
      541 aaaagtgtct ccatgatggc catgttaggt ggcgataagc gcctgattga tgcacataat
      601 caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg ggtgatgacg
      661 gacggacagt cagaaacggt actgaccggt aatctggtga tggcactctt taaccacgac
      721 accagtcgcg atcaggaccc acagttacac acgcatgcgg tggtggctaa tgtaacgcag
      781 cataacggcg aatggaagac actgagcagt gataaagtgg ggaaaaccgg tttttccgag
      841 aacgtgctcg cgaaccgtat tgcgttcggg aaaatatatc agagtgagtt acgtcagcgc
      901 gttgaggcgc tgggctatga aactgaagtg gtgggtaagc acggtatgtg ggagatgccg
      961 ggcgtaccgg tggaggcctt ttccggacgc tcacaggcta tccgggcggc cgtcggggaa
     1021 gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc caaacagcac
     1081 gtcgatcctg aagtcagaat ggctgaatgg atgcagacgc tgaaggaaac cgggttcgac
     1141 atccgggcgt atcgtgacgc ggcggaccaa cgggcggaga cccgcacgca gacacccgga
     1201 cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat tgccggatta
     1261 agtgaacgaa aagtgcagtt cacgtacacg gacgtgctgg ccaggacggt cggcatactg
     1321 ccgccggaag ccggtgtgat tgagcgggcg cgtgccggta tcgatgaggc catcagccgt
     1381 gagcagctta tccccctcga ccgtgagaag gggctgttca cgtccgggat tcatgtgctc
     1441 gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg ggtgactgta
     1501 catccggaga aaagtgttcc ccggacggcc ggttacagcg atgcggtcag tgttctggca
     1561 caggatcgcc cgtcgctggc cattgtgtcc gggcagggtg gtgcagccgg gcagcgtgag
     1621 cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca gattattgcc
     1681 gctgaccgtc gctcacagat gaacctgaag caggatgaac ggctgtccgg tgaactgata
     1741 accggacgtc gtcagctgct ggagggcatg accttcacgc cgggcagtac cgttatcgtc
     1801 gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg tgccgcacgt
     1861 cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggcacagg cagtgcactg
     1921 atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga gcagcgaccg
     1981 gtaaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc cggagatttt
     2041 gcggtcagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt acgggaacag
     2101 gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct cggacacccg
     2161 gaggtgacca tgacggcact gtcaccggtc tggctggaca gccggagccg ttatctgcgg
     2221 gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg cagtcatgac
     2281 cgctatgtta tcgaccgggt gacagcgcag agtcacagcc tgaccctgcg ggatgcgcag
     2341 ggcgaaacgc aggtggtgcg tatatcctcc ctggacagca gctggtcgct gttccggccg
     2401 gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat tcccggactc
     2461 cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc gatgacggtt
     2521 gttgtgccgg gacgggctga accggcctcc ctgcctgtga gcgattcacc gttcacggca
     2581 ctgaagctgg agagcggctg ggtggaaacg cccgggcatt ccgtcagtga cagtgctacg
     2641 gtttttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg tctggcccgc
     2701 agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc ggaaaaactc
     2761 gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc cggtgagaca
     2821 ttgctggaaa gtgccatcag tcatcagaag tccgcacttc acacgccggc acagcaggcc
     2881 attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat ggtggacctg
     2941 ctgacagagg cgaagtcgtt tgctgcagaa ggaaccagtt ttactgagct gggaggggaa
     3001 atcaatgcgc agataaaacg cggtgattta ctgtatgtgg atgtggcaaa aggctatggc
     3061 acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct tcgccatatt
     3121 ctcgaaggta aggaggcggt cacgccgctg atggagaggg tacccggcga actcatggag
     3181 acactgacgt cggggcagcg tgccgccacc cgcatgatac tggaaacgtc cgaccgtttc
     3241 acggtggtgc agggttatgc cggcgtgggt aagaccacac agttccgggc ggtgatgtca
     3301 gccgtgaaga tgctgccgga gagtgagcgt ccccgtgtcg tggggctggg gcccacgcac
     3361 cgtgcggtcg gggagatgcg cagcgccagc gtggatgcgc agacactggc gtcttttctg
     3421 catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa cacgctgttc
     3481 ctgctcgatg agagctctat ggtgggcaat accgacatgg cacgggcata cgccctgatt
     3541 gcggccggtg gtggtcgtgc cgtggccagc ggtgacacgg accagctgca ggccatcgcg
     3601 cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc catcatgaaa
     3661 gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat taaccgggat
     3721 gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc acgtctggag
     3781 ggtgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga agcgaaactg
     3841 gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatgt ccccatgaca
     3901 ctgtatgaag ccattgtccg cgactatacc ggcaggacac cggaagtacg ggagcagacg
     3961 ctgattgtta cgcacctgaa tgaagacagg cgcgtactga acagcatgat tcatgatgca
     4021 cgggaaaagg ccggtgagct ggggaaagag caggtcatgg tgcctgtcct gagcacagcg
     4081 aatatacgtg acggagagct gcgtcgtctc tccacctggg agaataaccc ggatgccctt
     4141 gccctggtgg ataatgtgta tcaccggatt gccggtatca gcaaggatga cgggctgata
     4201 accctggagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc ggtggctgaa
     4261 ggcgtcacac tgtacactcc ggacaccatc cgggtgggga gcggtgaccg gatgcgcttc
     4321 acgaagagtg accgggagcg cggctatgtg gccaacagcg tctggacggt gacagcggtt
     4381 tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg ccccggcctg
     4441 gagcaggcag agcaacatat tgacctggcc tatgccatca ccgctcacgg tgcgcagggg
     4501 gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa actgatggcc
     4561 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
     4621 gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac
     4681 gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg
     4741 gcacgggagc tgcgggatgt ggcagctggc cgtgctgttc tccgccaggc gggactggcc
     4801 gggggagaca gtcctgcacg gtttattgca ccggggcgca aatatccgca gccgtatgtg
     4861 gccctgccgg cttttgaccg taacggcaag tccgccggta tctggctgaa cccactgacc
     4921 acggatgacg gaaacgggct gcggggattc agtggtgaag gccgggtgaa gggcagtgaa
     4981 gatgcacagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct ggcggataat
     5041 atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt ggtgagaatc
     5101 gccggtgaag gtcgtccgtg gaatccctgt gccatcaccg gtggtcgcgt gtggggggat
     5161 atcccggaca acagcgtcca gccgggggcc ggaaacggtg agccggtcac ggcagaggtg
     5221 ctggcacagc ggcaggctga agaggccatc cgtcgtgaaa cggaacgccg tgcggatgag
     5281 attgtcagaa aaatggcaca ggaaaaacct gaccagccgg atgataaaac agagcaggtt
     5341 gtcagggcta ttgccgggca ggagcaggac agggcttcgt catcagaacg ggaaactgcg
     5401 ctgccggaga gtgtgctgcg tgagcctcaa cgggtgcagg acgctgtccg ggaggttgcc
     5461 cgggaaaatc tgctgcagga gcgacttcag cagatggagc gggatatggt ccgtgacctg
     5521 cagaaagaga aaaccctggg cggagactga tacaggaaga caaaggctga tgacaacgga
     5581 taacacgaac acgacacgta acgattcact ggctgcccgg accggtacca ggctgcagtc
     5641 gttgctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
     5701 ggtgctggac catgcaaacc gcattctgca tctggagcag acgtggatgt ttctggccgg
     5761 gaggggcgcg tttccgctgt ttgcgctggt ctggggactg aatcagtccc gtcatgcgca
     5821 tatccggcag tcggccatta accggctgtg gggatgggct gtgattgccc agttcgcgta
     5881 ttacgttgcc ggctttccct ggtatgaggg gaatatcctt tttgcctttg cggtggcagc
     5941 acagatgctg aaatggtgtg aatatcactc gctggtatgg tcagtcagtg cacttacact
     6001 tctggttctc tggttcccgt tatcttccac cagttacggt gctgtcggga tcatattact
     6061 gatgttctgt cacaggatat acaggacgac gcacctttct gtacggagca tgctggcgct
     6121 ctgtcttttc ccggtcgttg ctctcgttaa cctgcaggac agcgtgactg ccgctgtcag
     6181 tggtgtgtta gtgacataca tggtcgtcag gatggtatcc ggtatccggt ggcctgctgc
     6241 ccgtttctgg ccggcagact ttttcccggt attttatgtc tgtcatctcg ctgtgctggg
     6301 agtgatggcc ctgtagcagg aagcgcaaga aagcggatga actgtgaaag tctttttgtt
     6361 taaatcagaa cgcattcagg aaaaaacagg agcagatgat gaaaaaaata ttactggtgg
     6421 cagccattac gggaatgttc gttcaggggg tatgggcaaa atcagatcag caaatccggg
     6481 atgaggctaa aaataatgtg atcactgttt ctcaggtgag tggcatgacg gaggggaccg
     6541 gtgtgacgct gaatggtcag atagtgaagc aactggcagc cgaagaggat cattttgaac
     6601 tcagtgatgc atcaggaagt attgtcattg atgtcgatga tgaagtatgg cgcccgctgg
     6661 ccctgaaagc cggagataaa gttcaggttg tgggagaagt ggatatttct gatgagggaa
     6721 accggaaagc cacggtggat gtactgactc ttgagcgtct tcgctgatat gaacaacgga
     6781 taccggtgag ggcaggtgag ttatgacaga gcagaaacgg ccagtactga cactgaaacg
     6841 aagaaccgac gtggaaacac cgacccggag ccggaaaacc atcatcaatg tcaccacgcc
     6901 accaaaatgg aaggtgaaaa agcagaaact ggcggagaag gctgcccggg aagcagagct
     6961 ggcggcgaag aaagcacagg ccagacaggc gctgtccatt tatctgacgc tgccctcgct
     7021 ggatgaggcg gtgaacactc tgaaaccctg gtggccggga ttgtttgacg gtaacacgcc
     7081 caggcttctt gcctgtggta tccgggaggt tttactggat gaggtgtttc agcggaatat
     7141 cccgctctct cataaaaaac tcagccgggc gctgaaagcc atcacccgtt cggaaagtta
     7201 tctgtgcgca atgaaagccg gcgcctgtcg ctatgatacg gaaggctatg tgacagaaca
     7261 tatcacacag gaagaagaac agtatgcgca ggcgcgcctg gaaaaggtgc gcagacagaa
     7321 ccggatcaag gatgagctgc gggcgatcct ggcggaataa aagagccctc tgtatgacag
     7381 agggcaacag cgagaaaaaa tcgggaaaac agaaatccag agttttgatg gagaaaacac
     7441 ccaaccgttc agggatgttt cagaatgttt ctgacataat ccgggagccg ggtcaagagg
     7501 ggggataaaa aatggctgta tccagtggat tatctgagca cagcggtatt tatcgctatc
     7561 agcgcgtggg gcgtcatgcc gtccgtcagg agattcgtgg ttttttcagg gaagccgctg
     7621 aggcctggca gaatgcggcc cggctggcac cctgtgaagc atggcgggat tttgccctga
     7681 agagggcatc tgcatgccgg tgtaagtgcc gataaaaata acccccgtcg cggagctccg
     7741 ggggatcagg tggcagatcg ttattattat tatagtggtg taagaaggag ttgccctgtc
     7801 tttcaaacgg acatcacaga gttaacatat ttcacatccc gggtaagcca gcagggataa
     7861 aaaatgtgat gaatttcaag aagcaggagg tgacagtgga atgcataaca gcagggttat
     7921 ccggtgaatc tgacagacag ccgggcagta ttcatggcag gataaagaaa aaccgggaga
     7981 tgactctccc ggtccgtctt atcgctaatc ccctgagcag ggcatgtatg acttatctat
     8041 gaccgttata ttcagcgcca catccggtag cctgcgatat aacacagtta tcctatcagc
     8101 aagagcagga atgtttaata gtcaggggtg aaaaatataa tctgtatcac aaagtagtat
     8161 gtagcagggg gaagccgata gggtacgaac ggggaacggg aatacatgac cttgcgaaca
     8221 ggaaagtatg tatagtaaag tagagaagaa tggtattgac ataaaaatat caataaataa
     8281 attttaatca aagaagtata tcaatattct taatataaga tggggcaaac tccatcatat
     8341 ttatggaagt cagtgtgggt aaatcttgca acattgaata caaattatgg atataatcag
     8401 aaatgttcag atttaaccct tttattcgtc atcttctttc aaccagcata ggtgcatcgt
     8461 tattttcatt tcctgtgttc acttctgcac attcgacact tccggacgct gctgaagata
     8521 ctctggtagt tacagcatct gctgttgaac agaatctgaa agacgcacct gccagtatca
     8581 gtgttattag ccaggatgat ttatcccgta agccagtcca taatctggtg gatgttttaa
     8641 gggatgtgcc cggggtgcag attaccaccg atgataacag aaaactcgta aatataaggg
     8701 gactgggcgg aggatacacc ctcattcttg tggatggtaa acgggtcagt tcccgcacag
     8761 ctatgttccg gaataatgac tttgatctga gctggatccc tgtttccact attgaacgtg
     8821 tagaggtcgt gcgtggccct atgtcatcac tgtatggtgc ggatgcgatg gggggcgtca
     8881 ttaatatcat tactaaaaaa acaggtaagg aatggtcagg tgtcatctcg ggggatatga
     8941 cgttacagga acacagagac cgtggcgata cttatggggg acagttcagt gcaggtgggc
     9001 ctttggttga aaacctttta ggaattaaag tttacggagc tttaagtggc cgggataaag
     9061 ataccggaaa acaaaaatcg gcagatggac aggaaaccac acgtgctgaa ggttttacaa
     9121 cccgtaatgg tggcgttgac tttacgctta ctcctctgga gaatcacgag ttcagcgcag
     9181 ggtattcttt tgaccgtcag gaccgggagt ctgaaggtag tagtgataaa aaccggatgg
     9241 aacgacagaa ttattctgtt ggccataatg gacaatgggg attagggaac agtgaactcc
     9301 gattttacag tgaaaaagta aataatctga atacaggtaa tacacatccg ataacttcat
     9361 ggaatgaggt tattgatgga aaatatgtat tcccattcac tgattataat catatcgtga
     9421 tattaggtgg tgattatcgc agggagaaaa taagtgatcc tgtccacctt accggagaga
     9481 aaggttcaga aaattcgaca agtcagtatt cactttttgc agaagacgaa tggcgtttgt
     9541 tcgcgcccct ggctttgaca acaggggttc gtatggataa gcacgaaaat tatggaacca
     9601 actggagtcc aagagcatat cttgtatgga atgcaaccga ttctgttact gtaaaaggag
     9661 gatgggccag agcatttaaa gctccgaccc tgattcagca aagtgaagca tggctgacga
     9721 actcatgcaa agggggatgt cagatcatcg gtagccccga cctgaaaccg gaaacaagtg
     9781 aaagtattga gggtggtgtt tattacagcg gacaggaagg actcctggaa ggtgtggaag
     9841 caagtgtcac tgtgtatcgt aatgaaattg atgacatgat tgcgaacaaa cgcagtaaga
     9901 aactgaaaga ggcaatgacg ctgcctaact ttgtcgggat aacggatgat ggacggcctg
     9961 ttttcagata ctttaatata aataaagcta ggatacaggg gcttgaatca gaactgaaaa
    10021 tcccgctggg agagcaggtg aaactgtctc tcacctatac ctatacagat ggacgggatt
    10081 tgagtagcgg agaagataag ccactgaatg acagtccgtt tcataatgca aacggaagaa
    10141 ttgactggac tcctgtggat gactggtctt tttacgtatc agctaattac catggggaaa
    10201 cccgggcaca atcttccggc acagaaacgc agggggggta tacattatgg gatgccggag
    10261 cttcctggaa ggctacgaaa aatataactg tccgttcagg tatattaaat gttatggata
    10321 aagatttaga acggaatgat tacagttata ccgttgacgg gagaagatat tttctgtcaa
    10381 tggattatcg tttttaatag taaaccgaag gctccaagag gagcctttat aagataatgt
    10441 tctgaaaata aacaagacga taagaatgaa gtaagatgcg gatgaataaa gaaagtaaat
    10501 tatatttacg gaatcaggta aagttcattt ttataagaat tcattttctg cggtctttca
    10561 ttatgaagtt atcccatata tctgaaaggt aattaaggct actattaact aaaaaacata
    10621 cgttaacctt cgtacagaat gcatgattaa ttcatgagac cttagtaaca catggctata
    10681 tgtgtattca gccactgaat cttattttac tgaatagacc acaatttaac ggtttatttt
    10741 tggcgtgatg agtgaggcat tattcatcac ggaatggaat tcctgcaatc acaaaaagta
    10801 atcagtgacg gagagtaaaa tgaagaaata tataccagtg gtattgtttg ttttttcata
    10861 tcaggttttt tctgctgata ttcatggacg ggtcgtgcgt gttctggatg gcgataccat
    10921 tgacgtcatg ttatctcagc atccggtgcg tgtccgtctt gttaatattg atgcacctga
    10981 aaagaaacag gactatggac ggtggactga aaaaataatg taatctctgg ttgcgggaaa
    11041 aacggttact gtcacatatt ttcagcgaga tcgttatggt cgtattctcg ggcaggttta
    11101 tgcaccagac ggaatgaatg ttaatcagtt tatggtccgt gcaggcgctg catgggttta
    11161 tgagcagtac aacaccgatc ctgttcttcc cgtcctgcag gaagaagcac gacagcagaa
    11221 acgtggtctg tgggcagaca gcgcgcctgt tccgccctgg gtatggcgct cacggaaata
    11281 atgatatttc tgtggctatt gagttctcat tgtaatgcga tatttaaaag catgaagcat
    11341 atttcgttat taactggggt cagtttggat atagagaatt attgtacggt aagcctgttt
    11401 tttgaacagg cttacctata gcaatagggt gtactcccgc gctatacggt gttttcagga
    11461 gaattggtct taatcggata acctaaatgc gatacagcaa gagcgacaac ttaaaagcga
    11521 taactgctga cgtaaaatca atccgttaca catcgggatt ggttaaataa tgcgcttaac
    11581 gtacaaaaat ttccgatctc caaactgacc ccttaactta tctctctgtt aatgaaaggt
    11641 gaacgaatga actatcaggc agtatcagaa ttaataacat cgtctaatca taatgttctt
    11701 atagtggggg actcagcttc tgaagttgat agttttttaa ataaactgaa tatcactgat
    11761 tataagtatt atgatttcag gcaggtttac agttgttctg acagaacatt aaatgattat
    11821 gcggttattt ttatcaggga tgcactgaac gccagtgagc acattattat ttttaattgt
    11881 acaggatggc ctgatttgaa taatgaatct gccgtcatgc agtttgccag agtggcacga
    11941 aaatcaggaa aacaactgat agtcgtagtg agagaacagg atatgaaaaa aatggaagca
    12001 gagtttggaa ggatcataaa aatccactga cgccttcgcg ttgctcagtt gtccaacccc
    12061 ggagacggga aaaagcaagt tttccccgtc cccggcgttt ctgtagtgaa aagcgggaaa
    12121 tggagggaat ggtcagtaac agggaatgtg gtattatcgc ggcgggtgtc tgagcctttc
    12181 tggttcaggc aagacgcagg taccagaaat gcgaagaccc cacttgttaa tccattaact
    12241 cgtgaggcct gcatgaagta tctcaacact actgattgta gcctcttcct tgcagagagg
    12301 tcaaagttta tgacgaaata taccctaatt gggttgctcg ccgtgtgcgc cacggtgttg
    12361 tgtttttcac tgatattcag ggaacggtta tgtgagctga atattcacag gggaaataca
    12421 gtggtgcagg taactctggc ctacgaa
//
LOCUS       DAZGTJ010000118        12322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000118 DAZGTJ010000000
VERSION     DAZGTJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.118"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(362..1672)
                     /locus_tag="ACHK8E_004416"
     CDS             complement(362..1672)
                     /locus_tag="ACHK8E_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFP0346598.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(1685..2956)
                     /gene="dcuC"
                     /locus_tag="ACHK8E_004417"
     CDS             complement(1685..2956)
                     /gene="dcuC"
                     /locus_tag="ACHK8E_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0346599.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            3092..4009
                     /locus_tag="ACHK8E_004418"
     CDS             3092..4009
                     /locus_tag="ACHK8E_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0346600.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNCTSIDELATARWVFPETQMGYYHYISRLIPFGHPD
                     EPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLI
                     YPRKRLMTAPAQIMIEYFQQEIQRYDWNG"
     gene            complement(4258..4425)
                     /locus_tag="ACHK8E_004419"
     CDS             complement(4258..4425)
                     /locus_tag="ACHK8E_004419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346601.1"
                     /translation="MHASPVMTMVVCPTVLLCQNGHQKDQYRESLLSQMIASGHKELT
                     RVWQMNEIQAM"
     gene            4574..5407
                     /gene="iolR"
                     /locus_tag="ACHK8E_004420"
     CDS             4574..5407
                     /gene="iolR"
                     /locus_tag="ACHK8E_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFP0346602.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(5460..6893)
                     /gene="iolT1"
                     /locus_tag="ACHK8E_004421"
     CDS             complement(5460..6893)
                     /gene="iolT1"
                     /locus_tag="ACHK8E_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFP0346603.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
     gene            7332..8756
                     /locus_tag="ACHK8E_004422"
     CDS             7332..8756
                     /locus_tag="ACHK8E_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP0346604.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
     gene            complement(9000..9310)
                     /locus_tag="ACHK8E_004423"
                     /pseudo
     CDS             complement(9000..9310)
                     /locus_tag="ACHK8E_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9313..9480)
                     /locus_tag="ACHK8E_004424"
     CDS             complement(9313..9480)
                     /locus_tag="ACHK8E_004424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346605.1"
                     /translation="MPYTAACYSGFLTGSIRWAVYREIIRFSVEVEVEVEVEVEVEVE
                     VEVEVEGVYRS"
     gene            complement(9712..10521)
                     /gene="iolB"
                     /locus_tag="ACHK8E_004425"
     CDS             complement(9712..10521)
                     /gene="iolB"
                     /locus_tag="ACHK8E_004425"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFP0346606.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(10546..12051)
                     /gene="iolA"
                     /locus_tag="ACHK8E_004426"
     CDS             complement(10546..12051)
                     /gene="iolA"
                     /locus_tag="ACHK8E_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFP0346607.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQALGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT         3492 a         2601 c         2687 g         3542 t
ORIGIN      
        1 cagagtccag ccagtttgct catccgtcaa ttcaggtaga tgcgtcaggc tttgtggctg
       61 gcggaaaatg gctcgttcgc tgatattgtt gcgtcataca aaaacagtac gtgatgagta
      121 tgaggctttt ttatctgtat ataatctctt attctgtaaa ttaggatagg gtaatctcgc
      181 aatcatcctc ttcccatatc accagtatat gctgagccgt gtttgtctat ttggttaaaa
      241 ttcgctacaa tcctattctc tcttttggga atataaaagt ctccattttc tctttattcg
      301 ttaatggaga ctgtatttaa aggcagaaat caggagtgat tgacaggata cctgtcagca
      361 atcaatacaa ttcagattcc agttttccgt tccctacaat tttatttagt ccgggagagt
      421 tcaatctctc cagacataac gaaaacagtt taacgcctgt atccagcaca ttctcatcaa
      481 aatcaaaatc aacttcgtga tgaccagctt ttaaatcact gccaagtatt atatatgctg
      541 cttttcctcc tatcttctgt acacggcgaa taaggagcgt tgcatcttca ctgcccccaa
      601 atgatttctc tgtcaaagac atgttcatat cttcttgtgc tatatagtgt atcagattga
      661 caagtaaagg gtcattatgt aaatccgttg cttcaccaac cactttgtac tcaaaacgta
      721 aaccaaatat ggaagaaata ccttgtgcta ttttcagagc atgttcacgc atgtattgat
      781 ttattgtttc cgtttctcca cgaacttcaa gttgcatcct tgcaaaagac gggattatgt
      841 tccttccttc acctgcgttt agcgtgccaa tatttatccg ggacatacct tcaccatggc
      901 gtggaatggc aagtaattgc gttgcacagt gacaagcagc tgccagagca ttatttccca
      961 ggtgaggatt tacaccagca tgtgccggag taccatagaa aaagaaatcc atttttgtcg
     1021 tacacaacaa atctgtaggt gaagcgacaa catttcctgt aggaactccc ataccaatat
     1081 gtgaagaaag gaagtagtcg cagtcatcaa gaataccacc ttctgcaata ggttttgcac
     1141 cgcgaacgcc ttcttctgca gcctgaaaaa ttagcttaat tttcccgcat aacttatggt
     1201 tattttcaga tacccattgt gccaatccta aaccaatcga tatatgacca tcatgtccac
     1261 atgcatgcat taagccagag tgtatggatg caaatcccat tttttcagga atatgatctt
     1321 tcgattttga ttcacttacc tgtacacaat caatgtcaaa acgaaacgca gtgacagctc
     1381 cattttttcc tgtatcccat aaagcaacac accctgtaaa gccatccatt tcatccagca
     1441 attcatgggg aacgcctgaa tcaactgcac gttttatact atctgcaact tcagttgcat
     1501 tacggcccat aatatactct gttgacaggt atttattccc cgtaataacc tgccatccca
     1561 taccacgcag aatagtaatt atctttgacg ttgtgataaa ttcgcaccac ccactttccg
     1621 gaatatggtg aaattctctc cgccattttt gtaactgatt cagttcagac atatacgcct
     1681 cctgttaaag gaataataat gcaataaaca ataccgtcac aatcattccg ggggcagtgc
     1741 gttttgcaat ttcaactgga ttaactccag ccagaccggc acagataata gttgcaccag
     1801 ccaacggaga catgtttctc cctagcgcag aagccagcat aactgcagca cccagagtat
     1861 ttgtatcgta accaaaattg ttcgcatgtg gtgtgacaac ttcgttgaat gcgaatgcag
     1921 tcgcatcccc tgtgccggtt ataagtccca tcaggaatgg ccccaatgta ccgccccagc
     1981 gggcaaattc tggtgagtca gtaagaaatc tgataaacac atcaatgata ccaacagatc
     2041 ttaaaccttc aatgaaaaca gctgcggaaa ttatgatccc aagaatattt gcatatgatg
     2101 tccccatacc ctcaaagaag ctggaaacta attctttggg attggattta ctgacaatca
     2161 gggcataaag acaaccgaga atcattgcgg ctggaacggt tattttagtc tctccaagaa
     2221 aaccaaattc agccagcata agtaatgcta cgggaagaaa tggtgctaca gatttcagat
     2281 aaaaacagat atcacggtca gtgaactcaa cctgtgtttg ttctgatatg tttcctgcag
     2341 aatcacattt tgtttctttt cttacaatag caacaactgt aagaataaca gcacatatta
     2401 caccacatgt tattgtagtc ggagtatgga atacgacgaa ttccattaat ggtgtttgtg
     2461 atattttaga tataaatgaa ttatgtgcaa caccgggatt gaggtaagca ccgatagttc
     2521 ctgaaaggat agctgctccg gctattgcag gatgaatacg tgctgaaata agtaatggta
     2581 tcagcgttgc accaacagca gctgatgctc cagcagcaga tggaatcgcg atgcaaacaa
     2641 taaaactgac ggtaaccgca gcaggaataa gaaagatacc aagacgagat aatcctttcg
     2701 ttagaaaatg aaccaggcat gtatcacaac cagtgaattt cataacacag gcaaagccca
     2761 tactggtaca aatggcctga atgagaccgg actcagtcat tctgcttgca aatgccgaaa
     2821 atgcgtttaa aggtgacatg gcaactatac atagcagaat acctgcacca attagtgtcg
     2881 ttcgtgtgtc gtatcttttt atcaaaagca caactgtgat tatcagaatc acacatccag
     2941 ttatcacgtt aaacatgaga tcctccttct tcaaagaagg ataatcttta ctttatgtga
     3001 tttgctcaaa tattgatcat gaacaaaaaa atcaattaac cggggatact attatgatgg
     3061 aggtgataac agaaaattat caggcgaaaa aatgaaatat atgcctaaac ttcaacaaat
     3121 taaatttttc aatgaaatag tcctgtgcgg aagtttaaga gctgctgccc gtaaaatgaa
     3181 tatatctcag ccagcattaa gcagaacgat aaaagaactg gagaatgtat taggatgtaa
     3241 ccttctgatt cgaacaaggg atggtatcgt tttaacagaa gctggaaaga tattctcggt
     3301 ccattcacgt atgattcttg aagagattga aactgcagct cttgaagttg cgaaagtaaa
     3361 tatagataac acaggaaaag tagcttttgg aatttcttcg ctgtttggcg taacaatact
     3421 gaataatgta cttgataact tccggatgaa atatccgaaa aagcgtgtga tgataagaga
     3481 gggacagttg tcctccctta tccctttcct gagagaaggt gttctggatt ttgcaatggg
     3541 tacattaaca aaagacatgc cagttggaga ttttattgtg gttgaattgt ttaacgcacc
     3601 gttcactata gtggcaagaa aacaccatcc tttaaggaac tgtacatcaa tcgatgaact
     3661 tgcaactgca agatgggtat tcccggaaac tcaaatggga tactatcatt acatatcccg
     3721 tcttattccc tttggacatc cggatgaacc atatgctcca atatttaccg attcggtagt
     3781 ttgtattatg aatcttgttc tctatgatga ctatataacg gttctctcca gagcaaggtt
     3841 acaggaagca agattcaaag aaatattgtg ttcaattcct gttaagtatc aactacccgt
     3901 tagccagtac gggcttattt atccacgaaa aaggctaatg acggcaccag cacagataat
     3961 gattgaatat tttcaacaag agatacaacg atatgactgg aatggatgag caatacccgg
     4021 aatgatggtg tacaccggga taacaattta ttcggatgtg ccaatcccac ggttgtaatg
     4081 ggcaatataa ttgttagccg attcgctaat ggcagggaaa agcgcggcat aaatataagc
     4141 ccacacacat gtaacgtact gcagatactc gcttggcaga gttgttactt cttgggaggc
     4201 agtaccagcc atcagatatt cccgttccat gccaaaacct gagttacccg gataccatta
     4261 cattgcctgt atttcattca tctgccacac acgtgtcagc tctttgtgac ctgacgcaat
     4321 catctgactc agcagcgatt cacggtactg gtctttttgg tgaccgttct gacagagcaa
     4381 tacagtggga caaacgacca tcgtcatcac tggagaggcg tgcatggaaa ctcgtcaaca
     4441 actcaagttc gccggaacgg aacgttaaca atagaacttt actgtataaa tttgaatact
     4501 gctaaatgta tgaatggttc attcatgtta atattgtgat ttgcaaaaat ttcattttat
     4561 tagagtaaaa tacatgtcta aacaccaaac acagctttcc ttactgcagg acgacatccg
     4621 cagtcgctat gacagtctca gcaaacgtct taaacaagtt gctcagtata ttctggataa
     4681 cagtaatagc gttgcttttg atacggttgc ttctattgcg cagcacgcgg aggtacctcc
     4741 ttcgacatta attcgttttg ccaatgcgtt tggttttagc ggattcaatg aaatgaaaca
     4801 agtatttcgc cagcatttga tggaggaaac cgttagctat acagaacggg ctcgtctgtt
     4861 cagacaaaaa aacatcaatg atggggaacc cacaccggaa aaaccgggcg aaattctgaa
     4921 gctattttcc atggtaaatg cacaggtact acaacaatta ccgatgcaga ttaaaaatga
     4981 gcaactggat gctgcagtaa gtttattgtc aaaagccgat aatatttatg tgattggatt
     5041 acgtcgttca tttagcgtgg cctgttattt aacctatgca ttgcgccatc ttgagaggcg
     5101 tgcattttta attgatggta ttggaggcat gttttctgag caactgagta tgatcagctc
     5161 taacgatgta gtcatcgcca tcagttattc gccatacgct caggaggttg ttgaactggt
     5221 tgagttgggg gcaaaacgtg atgcacatca tatcgtcatt accgacagtc aggtcagtcc
     5281 gttggctgcg ttcagtgagg tctgttttgt aatacgtgaa gcgcaggtgg atggcttccg
     5341 ttcgcaggta gcgtcgatgt gtctggcgca gacgttggcc gtctcactgg ctctggctgc
     5401 tgaataagag gaagatgccg ataatctgtt tttcatgatc atcggcattt tggctaagct
     5461 taatctgaag agcgttcagc ctgaataggt tgtggcttgt tcctgcgttt tgtcagcacg
     5521 atatcctcca ttttctctag tgagacacct ttggtttccg gcaggtagcg gcaaataaag
     5581 aaatagctaa agacacagca ggctgcgaat atccacattg gaaatgcacc gtggaaatgg
     5641 gccagtaacc agggattttc gttaattata ggaaagaact gtgaaaccat aaagtttgct
     5701 atccacataa atcctacaga aatactcatt ccctgtgaac gcatgcggtt ggggaatatt
     5761 tctgatatga gcacccatgc tccaacgccc caggaaagtg catagaaaat cataaagaac
     5821 agcataccaa agagagcata atagccggtt gtttggctgt acagagccca tgaagttaat
     5881 aataaaccaa tgatgctacc aatggtgcct tttcgcatta gcgataaacg ccccattttg
     5941 tccatgatca ttgcgccaat aattgatccg acgagttgaa ttacgccaat ccagatggtc
     6001 tgaaacagcg cttcctgggc gctaccggta acatctctca atacaatagg tgcgtaatac
     6061 atcataacgt taacgccggt tacttgctgt aacattgcga tcatgcaacc gaggaaaagg
     6121 ataaaacgta cgttactgtc atggtaattg agcttttggt gggccgtcag ccggtcattt
     6181 tgcagagagg tttttatatc tgttagcaaa tgtcgtgcat gttcctcgtt agatatcttt
     6241 gtaagaattt ttagcgtttc ttcttcacga ccagccatca tcatccagcg gggggattcc
     6301 ggtataaaga atactaaaat acagaacaga atgcatgggg caatgccggc agcaaacata
     6361 taacgccagc caatctcaat cagccatgta ttggtcgcaa tcgaggcgat tttatagttg
     6421 acgtaaaata tgaggatctg accaaaaacg atggcaaact gttgcatact caatgccctg
     6481 ccacgcatgt ccttaggtga aacctccgac atatacattg gagatactgt tgctgcgagg
     6541 ccgacagcaa gtccacctac gatacgataa ataataaagt gcaggaaggt gtaggaaaga
     6601 gatgttccca ctgctgagat tgtgaacagc agggcggaaa tcatgagtga tttcttgcga
     6661 ccaaaacgtt tggataagta acctgagctg aatgatccca ccacacaacc tacaacgacg
     6721 ctggaaaccg cccagccggt ttcggccgga gataggtgaa aataactggt gagagaactg
     6781 attgcacctg aaataacggc agtatcgtag ccaaataata tcccgcctaa tgctgcaata
     6841 gaacagatac gtaatatata gcctgtattg taacgattca ctgatatggg catagctgat
     6901 tatctcctga gcagaaatca tctatctatt tgacctataa tgaaaaaatt gtgtttggtc
     6961 atttccgtag ctctgcagtt gtacttcttg ctttaccatc ggtaattaca tttcaatatt
     7021 ctgattggta attttcacta agatcaataa tgaaacattt attttgttat gtgaatcaca
     7081 ttttaaatta catgatctag gtgtttctcg gtgaatatat actcactcat caacaggtaa
     7141 cttttatcaa tatgaaaaat aatagttttt tattgttctg tattttcaat atgattattg
     7201 tgctacaaac tatttttcac agttatgaaa tttttatatt ttgtgatctt tgttgtggaa
     7261 tatttgagct gatttgttta agtatggaat aaatgttcgt atgtcatttc ctcaacaggc
     7321 agggaggggg tatgtttcag agaagcaagt atgattcagc ctatgtgtac gcattgtgct
     7381 gcgttgctgc actggctgga ttgatgtttg gctattcgac ggcagtgata acgggggtga
     7441 tactgccttt acagcaatat taccagctca cttcaactga aactgggtgg gcagtatcga
     7501 gcattgtgat tggttgtatc attggtgcgc tggtcggggg gaaaattgcc gataagttgg
     7561 ggcgtaagcc tgcgcttctg attattgcag ttatttttat cgtctcgtca ctgtgggggg
     7621 cgatgagtga atcttttatg gttttctcga tttcgcgcat cgtcagtggt tttgctgttg
     7681 ggatggcggg gactgcatct accatgtata tgtctgaact ggcaccagct gacattcgtg
     7741 gtaaagctct gggtatttac aatatatcag tagtttctgg tcaggtgatt gtttttattg
     7801 ttaattatgt gattgctaag gggatgccct ctgatgtgct ggtcgaagaa ggctggaaga
     7861 gcatgttatt cgctcaggtg ataccttctg ttacaatgtt agtgataact ttatttttgc
     7921 ctgaatcgcc ggcatggtgt gcccgtaata acaaggggga aaaacgttct ctgaaagtgc
     7981 ttgccagaat ctaccggggg gttagcgcta ccgatgtagc cgctattttt gatagtatga
     8041 gggaaacgga ggcccgggag aatgctgcag gcagggaatt cgttatcctg aaaagctctc
     8101 ctgtgttgcg ctatattctg ctggttggct gttgtatcgc tgttttacaa cagtttactg
     8161 gtgtcaatgt gatgaactac tacgcacctc tggtgctaca aaacagcagt acagaggaag
     8221 tgatgttcca gacgattttt attgctgttt gtaatgtgat cggtagtttt atcgggatga
     8281 ttttgttcga tcgctatgga cgaataccaa tcatgaagat tggcactatc gggtcaatta
     8341 ttggtttgtt gatcgcatca tatggtttgt atacccatga tactggctat atcaccattt
     8401 ttgggatttt attttttatg ctgttgtttg ctgtcagctg gagcgtagga gcatgggtac
     8461 tgatttctga ggtgtttccc gaaaagatta aaggattcgg catggggctg gcggttagtt
     8521 taatgtggat tgcgaatttt ctaatatcac tgttgtttcc ggtaataaat gataatacct
     8581 ggctgcagga aacctttggc ggagcgtttt ctatgtggat ttttgttgtc tttaatctgg
     8641 tttgttatgt ttttatttct cgctatgttc ctgaaacaaa ggggatcccg ctaacacaaa
     8701 ttgagcagct tgccggaaat aaactgcgtg aaattcaggg taaagctatg atgtaatagt
     8761 ctgattgtgt cacaatcagc agtaatgccc ggtaatgttg agcatcttac cgggattttc
     8821 cttgtgttaa tataggtaac aaaaggagat aataaatatc cagaaggttt tttataacca
     8881 actataaaat atatttctga ttgcatttga tacacgtatt ttatttggta tttgtgttaa
     8941 atttcattaa tttctattca taaacaggtt actctgatat gggagcaaca accattcggc
     9001 tatattatct ttttcatgtt ggtgctttta ttctgtgagg tgtgctggca tggtggacac
     9061 aaccatttgt tctttttagc tgccatttga taatattttc ctgaagcttt ccggggatat
     9121 attccccaga cgggagtggc ggcgctgacg gttgtagaat atctcaatgt atacccagta
     9181 ttactgagat gacttcattc cggctactaa aatggtagtg gttcagactt tcattcttca
     9241 gagttcccca aaagctttcc ataggggttt tgtcgtaaca gttcccttta cgagacattg
     9301 atgttttcag agctaactgc gatagactcc ttcaacttca acttcaactt caacttcaac
     9361 ttcaacttca acttcaactt caacttcaac ttcaactgag aaacggatga tttcacgata
     9421 gactgcccaa cgtattgagc ccgttaggaa gccagagtaa caagccgccg tgtacggcat
     9481 ttctctccta attcggcaga accaaaaata ttgtcagctc aggagtaaga atcagaaaaa
     9541 accataacag aacaccagaa acaggatatt atcagacaga gaaaaaattc aaaaaaatcc
     9601 accgattctg ttgcctgtgg atttgtgtat aagaggcaga accctgttgg ggactgtgca
     9661 caaaaaagcc agctgcagag gacaactggc aaaggaatat ttaacgtatt gttaacgcgg
     9721 atactctgac gaattaatcc atgcgtgatc agcttcccat gtgaattgcc atttgcgtaa
     9781 tggcccggcc atcacattca gataatagtt atcgtaacct gcgatagtgg ccaccggatg
     9841 atagcctttc gggactttta ccacatcgcg gttatatact gccatacatt cgtccagact
     9901 gcgatcggcg gtgtagactc gctgtaagct gaatccctgg ggtggatcga aacggtggta
     9961 gtaggtttct tccagatagg tttcctgccc ttcaactgct gtgtcatgct tatgggcagg
    10021 ccaggaactt gtatcgcctt cccttgtata tacttccact accagcagac tgtcagcagg
    10081 ttgagtgtct gggaggatat tatgaaccaa gcgctgattg cgcccttttc ccctatgttc
    10141 caccccaata tcttgtggtg agatcagtct taccggaagt tcaccgaatc caggagcact
    10201 gcagacagcc agttccagat cagtttcggc agtgactttt gcttctgtat ggtgcgggag
    10261 gtaaacagaa taagccggaa ttcgctcaaa aggactcatg cgctggccaa tccgataaaa
    10321 aaagtggtca gcggccttta cgctggcaag tccggcaacc agaaccaggc aacgttcgcg
    10381 atcatcggat ggcaaggtta aagattcgcc ggctttcaac agccagacat caaaaccgac
    10441 atactcccag cctgcgtttt gcggagaaat gtgttgaata tttccagccc tggtggtgca
    10501 ggtactcagc aagttggtca taatcttctc cttcctgaac gtttattaac ctagcgtagg
    10561 catactgaac tcggatgcga tctgctgacc gttcggccag cgtgcggtaa cggttttcat
    10621 ccgggtataa aaacgcaccc catccggtcc gtggacattc agcgccccaa acaccgagcg
    10681 tttccagccg ccgaaagagt gaaacgccat tggaaccggt accggaacat tgataccgac
    10741 catcccggct tcaaccgatt gagcaaattc gcgggcataa tggccgttgg aggtgaagat
    10801 cacgctacca ttgccaaatt catgagagtt taccgtacga atggcggtgg cataatctgt
    10861 tacgcgtacg atgccgagca cgggaccaaa aatttcctca cgccagatag tcatttccgg
    10921 cgtgacgtta tcaaacaagg taccgccgac ataaaatcct tcaggatagc ccttcacgca
    10981 ataatttcgc ccatccatca ccagtgttgc tccttcgcta acgcctttgt ctatatatgc
    11041 aaggactttt tgttgatgca tacgtgaaac aactggcccc atttcatttt cgtctttttc
    11101 tcgtaactca ccggggccaa tacgcagcgc tgcaataagt ggttttaggc gttcaataag
    11161 acggttggct gtttcatcac cgacagcgac caccacaggt aatgccatac aacgttcgcc
    11221 tgcggaacca aaagccccac ccataatagc gttaacggta gcatcaaggt cagcatccgg
    11281 catgacaata gcgtggtttt ttgcggcacc gaaagcttgg actcgcttac cgtgtgcact
    11341 ggcggtggtg tagatatgct cagcgacgga agaagatccg acaaaactga cggcctgaat
    11401 gcgtggatca ctggtcaaca ggctggctgc gtcattgcca cagtgaacta cattgaatac
    11461 accatccggt agtccggcct cctgtagtag ttgcgccata cgtaacgatg cggagggcac
    11521 cagcgctgga ggcttcagga cgaaggtatt gccgcaggcg agagcgatag ggaacatcca
    11581 cattggcacc attgcaggga agttaaaggg tgtgatgcca gctacaacac caagtgcttg
    11641 cattaatgaa aagctgtcaa cgcctgtgcc aacattaaaa gagaactctc ctttaatgag
    11701 atgcggaatg ccgcaggcga attcaacaac ttcgattccg cgagtcagtt caccaagcgc
    11761 gtcggaccat actttgccat gttcactgac gatcagagcc gccagttcat ctcggtgttt
    11821 ttccagcaac actttaaatt caaaaaggat tcttgcacgg cgcaacggtg ccattttact
    11881 ccatgcagga aaagcttcat gagcactctg aatcgcctcc agcatatctt gccgggtgct
    11941 ctgggtaacc aggcgaatca cttctccggt tgcagggtta gtcaccggta gtgtttcacc
    12001 actgttgctc agtctcgttt taccctgaat aaaatttcca actgtttcca tcactgttta
    12061 ccttcttgaa tcaatgttaa cgacgttccg actgagtgtt cacaggcaaa gcctgatcat
    12121 ctacagtcta aaatcacaca gtgtttaatg tatagcttcg gattataaat ttcaaattta
    12181 aatttatgaa aaaataagtt cactgcatgg ggggtgatgg ttataggttg cttgttgtta
    12241 actatttgaa tatcaaagaa aataaattgt gattctgtat tgttttattc gatgatctta
    12301 tccgtgttag atgagtttca cc
//
LOCUS       DAZGTJ010000119        12179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000119 DAZGTJ010000000
VERSION     DAZGTJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.119"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHK8E_004427"
     CDS             complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHK8E_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0346608.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQA"
     gene            complement(1913..2644)
                     /locus_tag="ACHK8E_004428"
     CDS             complement(1913..2644)
                     /locus_tag="ACHK8E_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346609.1"
                     /translation="MTSDNIAEYVGENNKFTERPVTEYPPMIQDNTATTKDYLYSNNI
                     DADLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEDCC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(2812..3072)
                     /locus_tag="ACHK8E_004429"
     CDS             complement(2812..3072)
                     /locus_tag="ACHK8E_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346610.1"
                     /translation="MNRAESRLSQVLFLLGYDREKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(3132..3863)
                     /gene="traT"
                     /locus_tag="ACHK8E_004430"
     CDS             complement(3132..3863)
                     /gene="traT"
                     /locus_tag="ACHK8E_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP0346611.1"
                     /translation="MKMKKLMIVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3912..4391)
                     /locus_tag="ACHK8E_004431"
     CDS             complement(3912..4391)
                     /locus_tag="ACHK8E_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFP0346612.1"
                     /translation="MKNLAHIALITVIQLIACNLADWGNAETVFILCFIVLWQGLFIW
                     LFSQIRKKRHVSNEFKFSKGIWYILMPASSLLSPLLSLMIFTIGTLYELRRVCGCVSV
                     REWMQSQVNEKTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYDE"
     gene            complement(4413..7256)
                     /gene="traG"
                     /locus_tag="ACHK8E_004432"
     CDS             complement(4413..7256)
                     /gene="traG"
                     /locus_tag="ACHK8E_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFP0346613.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVRVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLHSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVAIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLAATAGYISAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRST
                     RASAGMYGDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWEDNDAHTASSNTQSSHNAR
                     HDIDARATQDFKKASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEEKSAQEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            complement(7253..8614)
                     /gene="traH"
                     /locus_tag="ACHK8E_004433"
     CDS             complement(7253..8614)
                     /gene="traH"
                     /locus_tag="ACHK8E_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFP0346614.1"
                     /translation="MPALLLCSCLSFIPFTAVADVNSDMNQFFNKLGFATNTTQAGVW
                     QGQAAGYAYGGSLYARSQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQLQRFV
                     KQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGGLFPQ
                     TQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNINIIW
                     DSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGGTAKV
                     YHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGFISST
                     TVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYPQTVL
                     DSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNYHFGG
                     GTL"
     gene            complement(9059..9577)
                     /locus_tag="ACHK8E_004434"
     CDS             complement(9059..9577)
                     /locus_tag="ACHK8E_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346615.1"
                     /translation="MAGQQIFKRSVSCISLLSAVASGFYLLWSEWSAVKCQFDGMIFL
                     SFRNAGLFLLFFIILLIVVLFSVSMGVSGRYAVNPQIVTPGIIISLLLQCSAIMMVQA
                     WRWSPYALAGTFLLVQFWSGYLTGWVQRDLHEGRVKTGFRNTVSEFWLLIPLVLSGWG
                     ISWGYYTTLFTS"
     gene            complement(9593..10135)
                     /gene="trbB"
                     /locus_tag="ACHK8E_004435"
     CDS             complement(9593..10135)
                     /gene="trbB"
                     /locus_tag="ACHK8E_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFP0346616.1"
                     /translation="MFPVKSLSVVLLFLASCTQASTRAEIERLWHPEGMAARTTQPAA
                     DAQARQEKTATRWFRLSNGTQVNLADWKVVLFMQGRCPYCHQFDPVLKQLALQYGFSV
                     FPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGNKVSQ"
     gene            complement(10122..10412)
                     /gene="traQ"
                     /locus_tag="ACHK8E_004436"
     CDS             complement(10122..10412)
                     /gene="traQ"
                     /locus_tag="ACHK8E_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFP0346617.1"
                     /translation="MISKQRFSLPRLDITGMWVFSLGVWFHIVARLVNSKPWMAYILA
                     QVISAVLVLWGAYRVLDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            10494..10829
                     /locus_tag="ACHK8E_004437"
     CDS             10494..10829
                     /locus_tag="ACHK8E_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFP0346618.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSFPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFVIYDK
                     LAPVISLMK"
     gene            complement(10810..11154)
                     /gene="trbA"
                     /locus_tag="ACHK8E_004438"
     CDS             complement(10810..11154)
                     /gene="trbA"
                     /locus_tag="ACHK8E_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFP0346619.1"
                     /translation="MSEDSLNIVTGIVLLIFIIIVGYFFSERNDRKIFFLSSLVFLVI
                     NIVCLYVLTARFWFLCGAVMNQCAVPVVSMAVGTMPVITKFDRFRRIFVCIMLSSAWS
                     GVMWFFIISLVK"
     gene            complement(11286..11651)
                     /locus_tag="ACHK8E_004439"
     CDS             complement(11286..11651)
                     /locus_tag="ACHK8E_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346620.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCTLSMM
                     MAEGIVIMKYELWHIPPALLGLAGVLSWLLLLLHTGYFSGMSEGVNKRLWLTVATVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(11665..>12179)
                     /gene="traF"
                     /locus_tag="ACHK8E_004440"
     CDS             complement(11665..>12179)
                     /gene="traF"
                     /locus_tag="ACHK8E_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFP0346621.1"
                     /translation="FVKYFRLQNYWTQQAGLFTMSAKKAMLAHPELDYNLQYSHYNGT
                     VRNQLAADQAQQRQAIAKLAEQYGIMFFYRGQDPIDGQLAQVINGFRDTYGLSVIPVS
                     VDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQDDLAKQFL
                     NVSEDFKPNF"
BASE COUNT         3192 a         3154 c         2701 g         3132 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaaacaac ctccgggaca tccggttcga agagactggc
       61 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
      121 aggattgatg tcacgcggaa taaactccgg agcaaccttc ggtcgtgcct gatatttcag
      181 ggagagtttt actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag
      241 agactgaatg tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac
      301 cccgtcacgt accggatcag caccgtagga atactgctcg ctggctttca ggtgctcttt
      361 ctcaccaatt tcaccggcag cgaactctgc aatcttatgg ctgggggaac ggaaaaaggc
      421 acgggtgttc atgacgtcaa acagcgaggc tgccgatttc tcaccgtaga tatcttccag
      481 ctgggcatag gactggatac cgaacacata acagccaccg aacttacggg cttccggcag
      541 gatctccacc aggtccggca gtttgtgcag cgtgggtaac tcatcacaga agaaccacac
      601 acgacggtta cggttctccc ccatcgccat caggccacga atggcgatgg acagccacat
      661 ggagatcacc ggtttcaggg aagcatgggt gtcggcattc gacgaaataa acagccagcc
      721 gtttttctta tcttcacgga caccccgcat ccagtcacga atggtgaagg actcaccatt
      781 atgctcaatc ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat
      841 ggaaatcgcc gttttctcga ttttctcttc caccaggttg gccgccggtg agttacgcag
      901 gaaggtacgc agtttttcaa ttttgatgga aagcagtgtg tccaccagtt tgctgtagct
      961 gcggttgggg tcattgcgca tcaggtacgc agcttccgca aaaatggtgc gtcctgaacc
     1021 ctgccagaac gggtcctctt ttgtgcccat cgggatcagg gtatttgccg tattatcaaa
     1081 atccggctgt gtcaggcact ccttccacaa atcccaggcg gcacagcggg catccagcgg
     1141 attcaggata ttatcgatgg acgggtcata gtaactttta acaaattccc ctgatcggtc
     1201 ataaatcacc accatatcac cgcgctgacg ggcgtagttg gccagacgac ggataacttc
     1261 cgacttaccg gccccgaccg tgccgtgcag acagaagttc tggatttcag aatcccggat
     1321 aatcggcaga tcgccaatcc ggatatcgga gtccttgccg tcttttttca gcatcctggc
     1381 aacctctttc ggattgtcag tcagctgacg accacctgtg acttcatttt cactctgctg
     1441 tttaccctga cgtcccagaa tccaggagac aacaaagaag gtgatcaggc aaataaccag
     1501 ggccacgaca gacgccagaa cgaatgcaga ccatagctgc tcaccgcacc agatcatgta
     1561 tttatcatgc agtacctgag ccgcgttcat ccggaaggtt ttgccgtaat actggatctc
     1621 atagaccggc tgggacctga ttaaatcccg catgccttcc agcgtggtgc accaccagta
     1681 aatacagccg ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag
     1741 gatccagaaa aaaataaaca ggcagtaaag catgatattg gcgatctgac tgaacatgcg
     1801 gatacgcatg gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat
     1861 gaaactccgg gaaagtcagg aacagagcag aaaagagtgg tgtggaatac attcacagaa
     1921 caaaccatgt ataccctgta taccggtggt gatatccgcc tgctggcctg ccggaagaaa
     1981 ctttttccag cacgatatca gtcagacgca atatttcaga ccagtcactc tcgtgtgaat
     2041 actggatctg aacaaaaagg cgtacccgct cacgataatc cagagccgtg cccggttcat
     2101 aaaagtggcc actgtcagga gaaacacggg acgttttatt gatgagatcg tatttatcgg
     2161 catgagtctg tgacgacaga gaccaggtaa tattttcttc ctgctcacca tcaatacggc
     2221 tactcccggg cgtccaggca atttcctgcg cactggtcac gatgatctgt ctggagactt
     2281 ctgccagctt tttgggtgta cagaataccg gggccagcac acacagggac aacaaattac
     2341 agcagtcctc ttccggctta agcggattgc tcccggattt ctcaggaaaa gatatttttg
     2401 tcagtatttc agttaattcc ttatgcctga gagaattaat aaagtacatg acaagcatca
     2461 tgagaaacaa tataacaata agccctccga taaccactaa aaggtcagca tcaatattat
     2521 tgctgtacag atagtcttta gtggtcgctg tattatcctg tatcatcggg gggtattcag
     2581 ttacaggcct ctcagtaaac ttattattct caccaacata ttcagcgata ttgtctgatg
     2641 tcataatgac tttctccgga agcttccggc ttcgccggca acacttcacg gtcgcacaaa
     2701 gaatttgcac aaccgttcag tgttctcata tttatttcgg aaacgccggg aagtgacaaa
     2761 atttattttg ttactttccg gggttggaca acgagcaacg cgaggcgtca gctattttct
     2821 tctgcgacgt ccggtcggat attcactcac aggaagatat tctgttgcgc ttacgccgat
     2881 ctcatatgca gagaacggtt ccagtgctgt caggtatctc tggcaggcta tggccagttc
     2941 agaatctccg gttgcatcag caaaccggta aagttctttt aataactccc gacatacatg
     3001 ctcaggatac ttttctctgt catagcccag cagaaagagt acctgcgaga gacgggattc
     3061 tgcacggttc atgtattcat ccggggcagg aatatcggcc cggtacaatc tgtccgggcc
     3121 ggatgggtaa tttacaggat gttcgcgatg gatttagcca gctgatcttc cagtacaggc
     3181 ttcgcttctt caaatttcag gttaaccttg ttggcattag ataccacacg ggtctggtat
     3241 ttatgttgat ttccggttga tgtgctggtc tgcacttttg caccggaggt tccctgacgg
     3301 agagctgcaa cattatcagt tctgacttta gatgttgtcc gctctgcaat ctgcacatcc
     3361 gtgatcatgg tgtagttgat atcctccacc attgcatcgg cagccatacc aatcaaccct
     3421 gtggcaatcc ctacacccag cgccgcaccg gcagagctgg agttatatcc tgtgatgccg
     3481 gtgcccaatg ctgcaccggc aatggcacct tcataaccgc tgttcagcca ggtctgggac
     3541 tcccgcaaat ccattttgtc tgccttcagg acattggcct ggatccagta gtatgccttg
     3601 tcaggtgaag tcacaacctg gtagcctttg gctttaacgg catcactgat ttttgcctgc
     3661 aatccgctca tatccttgtc agatgtgttt ttgatttgca ggaaaacggt gcgttcactg
     3721 gaaggctcca gccagatggt ttcactcatc tgagttttga cttcaagatt acgcttcttg
     3781 attgctgtgc tcatcgcacc acaccctgaa aggaccagag tggaactgac cagggtaact
     3841 atcatcaatt ttttcatttt catcaatgtt ccttcatatt gaataaaaca ccataatata
     3901 caaattaaga ttcactcatc atatcgtttt gaactataac catatgaatt accaccgata
     3961 tccacaccaa tacctgacat aggtaaccct gttgcaggat tagtacgatg aaaatccata
     4021 ttatcaaaat ccagatgcaa atcttcatta gttttttcat ttacctgaga ttgcatccat
     4081 tctcttacac ttacacaacc acaaactcgc cttaattcgt atagtgtacc aatggtaaaa
     4141 atcatcaggc ttaataacgg cgagagtaag gaagatgctg gcatcagtat ataccagatg
     4201 cctttactga atttaaactc attcgacaca tgccgttttt ttcgtatttg cgaaaacaac
     4261 catataaaca atccctgcca tagtacaata aagcacagga taaaaactgt ttcagcgttc
     4321 ccccaatcag ccagattaca ggcaatgagc tgtatcacag ttatcagagc aatatgagct
     4381 aaatttttca ttatcatctc ccaaacccag cattattgct tatacttatc ctgatactct
     4441 tttgccctgt tcataagttc ctgtgggctg tcagcaccgg gcattccttc ctgagctgat
     4501 ttttcttcat tatatttatt attctgagat aatgcctctg ttttatggtg attttgcagt
     4561 tcagaatact gccctcttac aatattttcc tcgccgtgga tgctgttctg agtatctccg
     4621 atattgcccc gatattctgt gaccatatta tcaacctgct gcttcacatc gttacggata
     4681 ttactgtcct gcgttctttg ctcaataata ccctgatgcc cctgatggtc tgcaataata
     4741 tcctggctgc caccgcccga aggtacgctc tccattcctt taccgatatc gccacgggat
     4801 tcacgccagg cgttatcaac accaggctgt acctgttcct gaacaaaaga ccaggccatt
     4861 gcccgacgac gttctgcaat ctccggtgaa ctggtgtttg tcagaatagc ttctgcatcc
     4921 tgcggcgcgt gtttcatcac atactgtgca aactgttgtg acaggtcttc actcatctgc
     4981 ccactcatgc tttcagtacg ggaggccatt tcagcatatt catggctgcg ggtcatattc
     5041 gtcgtgtact ggtcgtaact ctgtttcgct gagttcaggg ctgcagacag ctggtccacc
     5101 ctggaatcgg cattattgtc agtatggctg ccggattcac tgaccttgcg actggtaaag
     5161 taatcgctgg cctttttaaa gtcttgtgta gctctggcat cgatatcatg acgagcatta
     5221 tggctcgact gagtattact acttgccgta tgcgcatcat tatcttccca atcaatacca
     5281 gcccgaccac cacctctgac acctgctcct ccaccaacac caagaatttt aggtttaatc
     5341 ccccattcag catgagcatc tccatacatc ccagcagaag ctctcgtact tctggatgcc
     5401 agctcctgag tcgcctgttc attactgata ttatgcgcct tcgcatagct ttccaccgca
     5461 ctgcgcatac ggctggccat catggaatcc tgcgcgctca tcgtactgtc agcgccactg
     5521 gtgactgaat cactgcttcc ccggttagag ccaaactggc tgagcgtgtt ccaggcactg
     5581 gcaatactgc tgctgaaacc atgcagggca ctttccgcct tattcatcga ctcccgggcc
     5641 atttcctgtt gcgctgctgc aatctgacgc gttgcattaa tgccaaccgg caaacgggac
     5701 attgccccac tggcgtccat caccatactg ccgtcacggg tctgcgtggc ggttgctcca
     5761 ctaccggtct ggtgcgtcat ctgaccaaat gacgtggtgc tgttggtgct ccagctgaag
     5821 ccgttcacgt tatttgtctg catgttgccg taggagtaat taccgtcaac cacactgcct
     5881 gccgcactgg cggttggcgt gatggaagaa gacgcaaagt gactgtatac actggagaat
     5941 ccggcaccaa ggcctcgtac catcatccat gacaacggag gtatcatggc agagatatat
     6001 ccggcagtgg ccgccaggtc agagtatttc agctgtatct gagagagttc agagagcacc
     6061 accggagccc cgttctgctt cgcatagaat gtcatggcac tgttcagaat ggcatacagc
     6121 aacggccagg tctggaacca catcagggca aacacatagc ctttcaggac ggacagcgtc
     6181 agtttgttga agaccgcagc cagcaccagg agcgggaaga taccaatagt aatgcccacc
     6241 agaatggtct gcaccatcgg caggtttcgc atcagcacat gaccaatggc tacatgcgcc
     6301 agacgctgtt tctccatcga tgaggtggtg gccagattca ccaggctggc cgtatcaccg
     6361 ttacgggcag cattactggt gataccctct ttcagggcat tcatggtgac gttctggcgc
     6421 ataatctggc tggcagactg gctggagccg tagaaatagc tgtaactgtc actcaccagt
     6481 tgtctgaata acaggtcagg gtccggtctg ccgccaaata tctgctgcac ataataatgc
     6541 caggtcttgc ctccggtatt cgtatcaaaa ttcagcttat ccttcagtcc gacggatgcg
     6601 tccttacagg acacaaacgg ctctgagctg tgcagaaaac tgtaattatt attcggcacc
     6661 tgacgtaaag ggctgggctg agaaaagatt atggtatacg ggtcagagga ggccatcagg
     6721 tcttccagcg tgtatttgtg gttcaggtaa atatcaccca gcacgcagtt ctggacgtag
     6781 tcctgaaaca gattgatgat ttccggattc cgggacagga aatcggtgct tttcacaatc
     6841 aggttcgccc cgaacagcat gcctgtcttg ctgtaggtgg cgctgtccgg ctgagcgaaa
     6901 accatctcat agccggccac cattgcatgc ccgatacggg ttgtcagtga aagtggcatc
     6961 gccagtccga ccggcacatt atccacccgg tgaaccctaa ccaggtcact gttatcaata
     7021 atctgcaccg atgtgcggac attaaccagc agactgataa gcacaaacac ggccacccag
     7081 cccagcaaat ccatcacgtt gtgccgctgt acccacatca cggccactgc gagaacagac
     7141 aatgtgagcg caatcttttc gatggaatcc cacgtccggg tccccataaa agcggcaatg
     7201 gcgttcaggt tattgcgcaa ccactcacca ccggcaatca catagacttc attcacagcg
     7261 tgcctccccc gaagtgataa ttgttctggt aacggctgag catgcgggcg gacaactgct
     7321 ggcgcatgta gctcatctga cggtcaacaa ccagcagtgc atcctgctgg acctttacct
     7381 gcccctggaa aaccgcaatc tgttgctgtg cctggttcag attctccagc acactgtcca
     7441 gcacggtctg tggatagttc ccggtggcaa tcatggcccg ggcctgctga agcagttcct
     7501 gaatgtactg gagcatgatg tcgtagccga tatagtccgt cagctggtaa accacgctgc
     7561 tggacacacc gagcatctgc gggtcaataa gatatttaaa gacggggacg gtggtgctgg
     7621 aaatgaagcc tttttccctg tcatccagcg gagtgtcact caccgcttta ttctgaatac
     7681 tggtgagcag ttttgtaatc tgtgatttca gggctttatc ccgggcgatg gtcacatttg
     7741 catccgccac caccttcaga cacttgtccg aatcgttgca gtgataaact tttgccgttc
     7801 ccccctccat aatcgcccgg aggatatcgc ggtcagtggt acgcgccggc aggggggtaa
     7861 tctcgctgtc ttccccaaaa atcagcgtac cggtgagcgt cataatgaac tctttcagct
     7921 ctttgttacc atcaagcatc ctgtttttgg acagcgagtc ccagatgata ttgatgttct
     7981 tcagcacccg ttccttatct ttgtcactgg ccttgtcacg aacagacgat gactgtcctc
     8041 cgaccgtgca gccctgacgg gacgcggccc agtcagcaaa aatattgctc tcaccggcaa
     8101 tgtcctgaca gaccttctgc tgtgacacct gagtctgagg gaataatcct ccgacaatcc
     8161 cctgtgccac ctgacaggag ctgaggttca tgctgttaat gtcgcttgcc atcttctgca
     8221 ggaaatcttt tgccgttttg atttccggca ccgttgtctg cagggcaagg tcaaaaaagt
     8281 aacccatcgc gttactcata atctgcttaa caaaacgctg cagctgctcg ccattaataa
     8341 aactgaatga gccgagatag gcatcgatgc caccacaccc tgcattgatg tccggcagtg
     8401 tcatggagat aagctggata tttttgacct ggctgcgggc atagagggag ccaccatagg
     8461 catacccggc agcctgcccc tgccagactc cggcctgggt ggtattggtg gcaaagccca
     8521 gcttgttaaa gaactggttc atgtcgctgt tgacgtctgc aactgcagta aagggtataa
     8581 agctcaggca actgcataac aataaagctg gaatgccacc tcgatttctg cataatttat
     8641 gtttaagcgt cggggataaa aacagattca taaacggtcc tctgtcagaa aacactgcgt
     8701 ttattaaaat taattaaaag ccatgaaatt attgttaaaa aagcatctga ttacctgaac
     8761 aataaagaca ttcaggataa tttacgaaac atattcagag atatattaac aggtatatca
     8821 ctgatagctc tcattacagc atccgcaata tggacaatta aggaacacgg cattaccggt
     8881 tgttttctca ttatccctca tgttcttttt tgtcctgttg tttttatatg gtgctgtttc
     8941 tgtattcagt caacatcaga aaagttattt tcgttaaaga agaaaagaaa ccgactttgt
     9001 gttgcactct gcattctgtt ttttcagttg accacaatct acagcatatt aaatataatc
     9061 atgaagtaaa taacgtggtg taatatcccc atgagatacc ccacccactt aataccagag
     9121 gaataagtaa ccagaactct gacacagtat tccggaagcc cgttttcacc cgcccctcat
     9181 gtaaatccct ctgaacccat ccggtcagat agccactcca gaactgaaca agaaggaacg
     9241 tgcctgccag tgcataagga gaccagcgcc atgcctgcac catcataatt gctgagcact
     9301 gaagaaggag agaaattata atccccggag taacaatctg agggtttact gcataacgcc
     9361 ccgacacacc cattgagaca gaaaaaagaa caacgattaa cagtataata aaaaacaaca
     9421 aaaacaatcc ggcattcctg aatgaaagaa aaatcatacc gtcgaactga catttcaccg
     9481 cagaccactc gctccagaga agataaaatc cagaagcaac agcactcagt aaagaaatac
     9541 aacttacact tcgtttaaat atctgctgac cagccataaa ttctccctga tgttattgcg
     9601 aaaccttatt tccgtacatc tgcagaacgg tatccatccg cgccataaaa cctgcagcat
     9661 ccgttgcccc ctgtaaaagc ggtaatgcct caagcgtatt gacgttaacc aggaaggtgg
     9721 tcggtgtggc caccgggata ttcgggaaga atgttttcag tacatccggt ggtgcaggta
     9781 acgcctgcgg gaaggccgta tcaccctgac catccagggt atagggaaat acggaaaatc
     9841 cgtactgcag cgccagttgt ttcagtaccg ggtcaaactg gtgacagtac ggacatcgcc
     9901 cctgcatgaa cagcaccact ttccagtcag caagattgac ctgagttccg ttgctgaggc
     9961 ggaaccagcg ggttgctgtt ttctcctgtc gcgcctgtgc atctgctgcc ggctgagttg
    10021 tccgtgctgc cattccctcc ggatgccaga gtcgctcgat ttccgcacgt gtggatgcct
    10081 gtgtacagct ggccagaaaa agcaggacaa cggacagcga tttaacggga aacatgttca
    10141 tcatcattat ccttccgttc tctcagtaac aactgctgtc tgacttccag tgcctcacgc
    10201 tcttcctgac tgacacgggc tatccacgta tcaagcacac gatatgctcc ccacaggacc
    10261 agaacagccg aaataacctg agccaggata tatgccatcc agggcttgct gttaaccaga
    10321 cgggcaacaa tatggaacca gacacccagg gaaaataccc acatccccgt aatatccagc
    10381 cggggtaaag agaatctttg tttacttatc atttgaatat cctgttgtgg tttacatgaa
    10441 tttattcacg catataatct gcttaacttt cgtgaaatta atcagtgtgt tatatgaaaa
    10501 agttatcttt caaagaaaaa ctggaaatag tgagaaacat cataagagag tcattatcag
    10561 gtaatgcggc ttttgcttta ctggtgtatt cattattgca ttccttcccc attaaaacct
    10621 ttgctgattt atttgtgacg atactgttat ttatagcaat tatcgctgtt gtgatatggc
    10681 tatttctcat catctatgtt tatttttgtg aattattcag aagtcactgg atagcagtct
    10741 ggtttatcct ctggtctgtg gtaatcaacc tcactattgg atttgtcatt tatgacaaat
    10801 tagcaccagt catttcacta atgaaataat aaaaaaccac attactcctg accacgctga
    10861 tgacaacata atacagacaa atattcttct gaacctgtcg aacttcgtga taaccggcat
    10921 ggtaccgact gccattgaaa caacgggcac agcacactga ttcataaccg caccacaaag
    10981 aaaccagaat ctggcagtca gcacatataa acacacaata ttaataacaa gaaaaaccag
    11041 cgaactaagg aaaaatatct ttctgtcatt acgctcagaa aagaaataac caacaataat
    11101 gatgaatatt aacaaaacaa tacctgtaac aatatttaaa ctgtcttcac tcataaatta
    11161 aacccgcgtt ctaaaattcg gtttaaaatc ttcagatacg ttcagaaact gtttagccag
    11221 gccgccctgt gaaaatttgt aacatttatt ctctggttat atctctttct cgcattggcc
    11281 attcattatt tcattatggt ttctgatggc tggagtaacc agtagaatat aatcattcct
    11341 gcaccagaaa acactgtggc aacagtgagc cacagacgct tattcacgcc ttctgacatg
    11401 cctgaaaaat atccagtatg gagtagcagc agcaaccatg acaacacgcc tgccagcccc
    11461 agcagggcag gagggatatg ccacagttca tatttcataa taacaatacc ttctgccatc
    11521 atcatggaca gggtacaaac aacaaaacag tcccatgaaa aaaaacgcct gtcagcagca
    11581 aggaaaatca ctgtcagtga catcactgtc acctgggcca gcaataaagg ccataatgaa
    11641 ataaaattca tttaaacgac ctctttaaaa attcggttta aaatcttcag aaacgttcag
    11701 gaactgtttt gccaggtcat cctgcgaaat aaaaccgtat gataacgggc gaacactgcc
    11761 ctgtttcggg tctaccagca tcatggccgg gaaatatttc acaccgaggc gctgcgcctg
    11821 cccctggtca gtccgggagt ccggcaacag tggattaatc acgccatcca cggaaacggg
    11881 aataacactc agaccatacg tatcccggaa gccattaatg acctgcgcca gctgcccgtc
    11941 gatggggtcc tgcccccggt aaaaaaacat aatgccgtat tgttcagcca gtttcgcaat
    12001 ggcctgtcgc tgctgtgcct ggtctgccgc cagctggttc cgtaccgtgc cgttgtaatg
    12061 gctgtactgc aggttatagt ccagttcagg atgtgccagc atggcctttt tggcgctcat
    12121 ggtgaaaagc ccggcctgct gagtccagta attctgcaga cggaaatatt tcacaaaat
//
LOCUS       DAZGTJ010000120        12135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000120 DAZGTJ010000000
VERSION     DAZGTJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.120"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(88..825)
                     /locus_tag="ACHK8E_004441"
     CDS             complement(88..825)
                     /locus_tag="ACHK8E_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0346622.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
     gene            complement(923..1116)
                     /locus_tag="ACHK8E_004442"
                     /pseudo
     CDS             complement(923..1116)
                     /locus_tag="ACHK8E_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(1536..1718)
                     /locus_tag="ACHK8E_004443"
     CDS             complement(1536..1718)
                     /locus_tag="ACHK8E_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346623.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            1657..3744
                     /gene="iha"
                     /locus_tag="ACHK8E_004444"
     CDS             1657..3744
                     /gene="iha"
                     /locus_tag="ACHK8E_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP0346624.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            complement(3962..4112)
                     /locus_tag="ACHK8E_004445"
                     /pseudo
     CDS             complement(3962..4112)
                     /locus_tag="ACHK8E_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4606..4848
                     /locus_tag="ACHK8E_004446"
     CDS             4606..4848
                     /locus_tag="ACHK8E_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346625.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            5139..5507
                     /locus_tag="ACHK8E_004447"
     CDS             5139..5507
                     /locus_tag="ACHK8E_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346626.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            5511..5723
                     /locus_tag="ACHK8E_004448"
     CDS             5511..5723
                     /locus_tag="ACHK8E_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346627.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            5720..5992
                     /locus_tag="ACHK8E_004449"
     CDS             5720..5992
                     /locus_tag="ACHK8E_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFP0346628.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(6301..7132)
                     /locus_tag="ACHK8E_004450"
                     /pseudo
     CDS             complement(6301..7132)
                     /locus_tag="ACHK8E_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7190..7378
                     /locus_tag="ACHK8E_004451"
                     /pseudo
     CDS             7190..7378
                     /locus_tag="ACHK8E_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7447..8673)
                     /locus_tag="ACHK8E_004452"
     CDS             complement(7447..8673)
                     /locus_tag="ACHK8E_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346629.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(8746..9768)
                     /gene="iolG2"
                     /locus_tag="ACHK8E_004453"
     CDS             complement(8746..9768)
                     /gene="iolG2"
                     /locus_tag="ACHK8E_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFP0346630.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(9941..11881)
                     /gene="iolD"
                     /locus_tag="ACHK8E_004454"
     CDS             complement(9941..11881)
                     /gene="iolD"
                     /locus_tag="ACHK8E_004454"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFP0346631.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         3399 a         2996 c         2677 g         3063 t
ORIGIN      
        1 ccgtagttac tacagtgctc cggagtgata aacagaaact gataatgaag cataagaata
       61 cttttattat gcgctgactg acatacttta cttaattgcg attctgctct caataaatcc
      121 ggactgatta atcatcatct gatgaggtgt tataccaaaa tattctctga aaacgctgat
      181 aaaatacggt gtgctggcat aaccgcaacg tattgcgatt tgattcactg acagtttact
      241 ggaaataagt tgtcgggcgt tatgcatgcg tatatccagc aggatttggg agaaagaaac
      301 tttttcctcc tgaagttttc ttttcagtaa actctcactc atgtacagag aattactgat
      361 atctttaact ctccatgccc gtgatggact ggaacagata atatacctga ctttaagcgc
      421 aacagagttt atacatctga atagcagaaa catgaaacct ctgtgtgtgg aaaaaatgga
      481 aaggcaggca aaaaacatct gctcagaaaa ttgttgtggc aatgtctttt gcagactgag
      541 attctgcatg acattatgta acagagcgat gtgagagaag gttgttttaa tgaacggaac
      601 agaaacagca ctatcagagt gctggatcct gtcggcactt tccatatatt ttctgacaat
      661 gcattcgtca atccggagct cagtaacatt accgggatgg cgcaggaaaa aggactggct
      721 gatgttatct gtcaacagga cgtcattttt ctgaaaaaat agctttttcc cggcaatctc
      781 tgtttcaaac ggacggttaa caagaataac gtaacagaca atcatcacac atactccctg
      841 attagcataa aaacaacgat atcaaaagac tagacgcttt ttcaggaaaa atcttatttg
      901 tccaggatta ttcttacgag accggcgctt caacggtacc gtcacatttt ttacaggctt
      961 tttttacccg ttctgtgctg atcactttca gggagctgct caccagttcc agttgttcag
     1021 cgctgacttc cccagataat ccagctcacc gccacactcc gggcaacagc tttcttcagg
     1081 ctccagccgg tgtatttcac ggggaaggtg tgccggtgtc agcgctagtg gtataagacg
     1141 gttctccctc atcgtttgtc cttagtattg tcagcgccaa caatgtgggc tggcattata
     1201 aatgccgtcg aggcaatcgt tcgcctcccc agtgggcaat ccaaatggtg attgtcacgg
     1261 ttagacgttt attttgaagg tcgactgaat aaggtgataa cgctgtagta ttttcacgtg
     1321 acacagaatt ctgaacgttc tctcttacaa tatttcaatg tttctgtccg tattcaccgg
     1381 aagaagtcat cgaccaaatc ataccagtcg tctcactcca ctgaccattc atggttgaca
     1441 ggtggtgaaa atccgcttct gcagtaacca ttttttattc gcaaaaccga acacgccatt
     1501 gcggatggct gttaactggc atgccgaggc agtcattatt tatatttagt tttgtcagta
     1561 atattcattt tttgtaaaag gtaaatataa attattctca ttattgtttg tatttgtgta
     1621 ttgtcttgcc gattaacatg atcggagatt agtaatatgc gaataaccac tctggcttcc
     1681 gttgtcattc cctgtctcgg gttttcagcc agcagcatgg cagcagagga tgtgatgatt
     1741 gtctcggcat ccggctatga gaaaaaactg accaacgcag ccgccagtgt ttccgtaatc
     1801 agtcaggagg aattgcagtc cagccagtac cacgatctgg cggaggcact gagagctgtg
     1861 gagggtgtgg atgttgaaag tggtacgggt aaaaccggtg ggctggaaat cagcatccgt
     1921 ggaatgcccg ccagttacac gctgatactg attgatggtg tccgtcaggg cgggagcggg
     1981 gacgtgacgc ctaacggttt ttctgccatg aatacagggt tcatgcctcc tctggccgcg
     2041 attgagcgta ttgaggttat cagggggccg atgtccacac tgtatggctc tgatgcgatg
     2101 ggtggtgtgg tgaatatcat taccagaaag aatgcagaca aatggctctc ttccgtcaac
     2161 gccgggctga atctgcagga gagtaacaaa tggggcaaca gcagccagtt taatttctgg
     2221 agcagtggcc ctcttgtgga tgattctgtc agtctgcaga tccgcggcag cacacagcag
     2281 cgtcagggct catcggtcac atcactgagc gacacatcag ccacgcgtat tccttatccc
     2341 acggagtcac agaattataa tcttggtgcg cgtattgact ggaaggctac ggagcaggat
     2401 gtgctctggt ttgatatgga taccacccga cagcgttacg ataaccagga tggtcaactg
     2461 ggtagcctga cagggggata tgaccatact ctgcgctatg agcgaaacaa aatttcggct
     2521 ggctatgatc atactttcac cttcggtacc tggaaatcgt atctgaactg gaacgagacg
     2581 gaaaataaag gtcgtgagct tgtacgtagt gttctgaaga gcgataaatg ggggcttgcc
     2641 ggtaaaccgc gggagcttaa ggaatctaac cttatcctga attcattgct gcttacccct
     2701 ctgggagaat ctcatctgct tactgtgggg ggagagtatc agagttcgtc catgaaagat
     2761 ggggttgtcc ttgccagcac aggtgaaact ttccgacaga aaagctggtc agtatttgct
     2821 gaggatgagt ggcatctcac ggatgtactt gcgctgactg caggcagtcg ctatgaacat
     2881 catgatcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga cgtgaccgat
     2941 gcctggacgc tgaaaggcgg tgtgaccacg ggatataaag cccccaccat gggacagtta
     3001 cataagggga tcagcggtgt atccgggcag ggacgtacta atctactcgg taaccccgaa
     3061 ctgaagccgg aagagagtgt aagttatgag gcgggggtat attacgataa cctctccggt
     3121 ctgaatgcca atataacagg ctttataaca gacttctcca acaagattgt ctcttattcc
     3181 attgatgata acaccaatag ctatacaaac agcggaaagg cccgcttgca tggtgtggaa
     3241 ttcgccggca cattgccgct gtggtcagag gatgtcactc tgtcgttgaa ttacacctgg
     3301 acccaaagtg aacaacgtga tggtgataac aaaggtgcac cgctgagtta tacccctgaa
     3361 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
     3421 gcccgttatc gcgggaaaac accacgtttc acccggaatt attcgtcact gagcgctgta
     3481 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
     3541 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     3601 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagttcgct gtatgccggt
     3661 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     3721 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     3781 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     3841 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     3901 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     3961 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     4021 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     4081 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     4141 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccgggggc ccggtcagat
     4201 aagtcccttt gtctatcgct gactcctggc tcataacccg gttagcagaa tgcagggtca
     4261 ccacctcgcc acgaccgaat ccggataagt caattgcacc ttctcaatcg ccattttgtc
     4321 acaattaagt ttgataaagc aggtcacctc tgcactgaat ctgctccagt gcctcattgg
     4381 tctccgtccc cccctttaaa ctgccggata agactgctgc acagcgcctt ccccggaatc
     4441 aaatcacttt ccctgtccgc cacaatccgc ttcatgctac ctcgtatttt gtttcagaga
     4501 ccggaatctg ccagaaagtg actacgcgtg cacatatcgg ttttctgggt tacatgcatc
     4561 gtgttatttt ctgaaatgca aaataataaa aaacaaggac acataatgaa ggatgaaata
     4621 cacagtacca gcatcaaccc ggatgttgtt gagcagtttc tggcccttat gaacacacat
     4681 tgtaaatcct cagaagaggc gctcgccatg ttgagtacgc tttgttacaa cctgatggtg
     4741 acatgtggca tccattccat ggacagccag atgctcaatg ggcatgggat atccatgcaa
     4801 cttcagggga ttgatattgc cgaaaggcca ggtaagaata ttcactaacc tgaacagggc
     4861 ctgaaaacgc tgtcttttca ggcctctgca ggcaaaaact gatgacaaca aaatttctgc
     4921 gtttgtaccc caccgatgaa aaagacaaaa actcacaccg ggcttctagc cagtaaagat
     4981 aaaacccgac gtgtcagcct ctacgaaacg ccaacggcat ggtgtatcag ggggcaggag
     5041 tgctacagca aaagtacagg acgccgctgt ggcagccacg acagtctgag cagactgcgc
     5101 ctagacagca ttaagcctgt tgaatgagtg gtggcaccat gaaagataac aacacaaccg
     5161 ccaccccaga agcctggaaa atgcgttttc tggccaacgg gacatccggc acatggtttt
     5221 acactgacga cattgcagtc actgacgcgt ggcttcgcgc gcacccggat gatgtggagg
     5281 ttatccctct ctgccggata ccaccccgtc agcattccca tgaacaacat attgaagaac
     5341 tccgcgcagc cctggcccgg atgctgcata tgcatcatat gatgctggaa accacgcatt
     5401 acagggaaaa catcaccgat gcccgttgta tccgtgaaat gcatgaagct ccattacagg
     5461 cgctcaatat tctggccaga tacccgcata aaaaggacga ggtgtgacgc atgtggctac
     5521 tgattttctg gctttcatct ccctttaatg tctcttccgg tgatgcccgg cagcccacac
     5581 tggcggtgca cagccagaca tttcataccg aacgggcctg tcgcgatgca ttgcgcgcca
     5641 ttaaaaagga gaatgttggc gaattttctc tgcggggcgt ttgtgtttct gacggaaaca
     5701 cgaaggaggc agagagctca tgacggatat tactgacaga catacacttc gccgcatgtc
     5761 ctggtcggaa cttaccacgg cagcgcagga agcagaatta aaccgtgatt acgagcgtgc
     5821 cagaatagtg tggtcattcg ccttacatgt tgcaacaacc acgattaaca aaaatctggc
     5881 catagctcat attcgacgct gcgatacttt gttgcataaa tcaaaaacag tacccggaaa
     5941 caacccgaga ggccggtcag tgtgcctgag acctcaacat ccccggagat gactgacatt
     6001 ccggactgac gcagttgctg catatctgtg gcaatacgtt gtatccagcc agacaggcta
     6061 tcccgcgtag tgacaattaa cccgctttcc tgcaccagct tcagccgggc cgtcggggat
     6121 tttaccagcc ggaaatatca gtaaaacaac atatcattat gccatggtgt acgcggccag
     6181 tgtatgctta cttaaggaat gagggataaa ttggatatta ttacttcact ttgctttttt
     6241 atgagattca tcacataagt ggggttttac tgttaaataa tctgaattta tttgatatcc
     6301 gtaaagcaga tgtctcagtt cactgtttcc aacttttgtt attcctctgg ggcgaatgct
     6361 gtttccgctg gaatattcac ctggcaccag tcccagaaaa gccatgagct gttcaggatt
     6421 ggaaaatcgc gaaaaatcac ccagttcagc aaccagggtt attgcgatta gctgaccaac
     6481 acctttaaga gcctgcaact ggcagaccag attgcacaga gatcactcag gtaacaggcg
     6541 agatatttcc tgatcgagtt gaagtatgcg atcttcaatc tgctccagat tctgacaata
     6601 atgttgaaag caatctgttg agatagcaga ccaaatgatt gattaactaa ccaggttcga
     6661 tgtttgccgg ttcagtgctt cctggcataa cgtttgtctg ttcgtgtcac gttttgcagc
     6721 ggcacgggct ctgattaaat cccgcatagc ttcatgagtc agctcaggga gccagactgg
     6781 gggaacctca ccatccctca gtaacctggc gagagaaatt acgtcccgat ggtcattttt
     6841 gatcctgtct gccggtgatt tgggtatacg ggaagggggc aataaccagg cattttatat
     6901 tcatcactgt gagttgatga tatagcccaa acccacaggg accaccttca tagcaggaaa
     6961 gaatgtttcc gtaaggtccc tgcaatttct gacataatcg atgtacagcc aaggcttcgt
     7021 tgttaatcgt accataataa cgaacttcgc caagccattc aggtgtagca atcgccaagg
     7081 tttctttatg aacatccagc ccgacgtaag ctgcaatatt ttccgaatcc atgatgacct
     7141 ctggagtatc ccaccaatgc tatagtgagt atataaaggt catatcatct acgtgttctt
     7201 cggcaacgat cacagtggag agcgtggagc gctactgtac gggctgattg gcacctgccg
     7261 actgaacagt atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg
     7321 gccttccaac cgtgttgacg aactgctgcc atggaacgta gttctcaccg ataaataagc
     7381 gtcaatacgg cattcgatta atgcacttac agctaaatgg agtttttgaa caatatttga
     7441 cgttatttat ttctcaccag gaactttaag aaactgaacc aggatgccac taaataaata
     7501 gagtcctgcc atagtataca taaccacgcc tgtaccaaaa cctgtcagaa tggataccaa
     7561 cgcaggtccg acaaaatttc ccaggccaga accgaaattc accgctgagt ttgctgcccc
     7621 cttcttatcc ggtgccatca tcggtaccag cggcgtcatc ggcacaaatc ccgccagccc
     7681 cgctccccac aggcacattg ccagcataaa agcaacgtaa ttatgccccc acactatcgg
     7741 cacccagtag acaatcagcg tggcaaaccc ggtcagaaga ctgccaaaaa tctggatagt
     7801 acggcgaaaa cctattttat cccccatcca gccgaaaata atgttgaaaa tctgattaga
     7861 aataaaggtt ataccccaga tactcgccca ctcctcaagg gtaaactgtt tatccagcaa
     7921 aaacagcggc aacaccacta caaaaccata ggtgccgagt ccattgattg ttttaacaat
     7981 cactgccaga ccaattcggg gtcgttgata cataatggaa atacctgccg acaactcttt
     8041 aaagactgac gtagttcttg gcttcagaat gaacgcattg cgctcaaaga ccagcatgaa
     8101 aatcccccca agcagtgcaa ccccaatccc ggcccacata acattaatat gcccaaggac
     8161 aggaattaac gaagcagcta ctaccggacc aatgatggta aaacctaagt tatatactac
     8221 ccaaaacagc gacgttccaa ttccctgttg atgattatcg agttgaatgg tcagtcggac
     8281 aagaaaggaa tagcagacca acggataacc tactccccgc agcatatagc taatgagaat
     8341 tgtactaaaa tcatggctgg gaatcgcaaa attaagaaac gcgatagaag cgatcaggta
     8401 aacggagaaa ccagcaagca taacctttct ggggccaaac atatccacac agacaccgga
     8461 taaccaggaa gttacggcaa ccacaattcc gtaggcggca aatatctgtc cgatctgaga
     8521 ctgatcaaat ccctgagtat tcagccaggc agatagccaa ctattttcaa tacagccgcc
     8581 caacataaat aaaaatgttc ccaggtagct ccagataaac tgacctggct ttatataaaa
     8641 tgtctgaggc aacattgcct gcatatcttt cattttataa ccctcagtac ggataatctc
     8701 tcgctcgtaa tagtgcgaga gacccttcgc tatttacaaa tgcaactaaa gttgaatatc
     8761 agtttttatc ttcactggca gtccactttt taatgattgt tttgcagcaa tggcaatacg
     8821 ctgcgccaat aatgcatctt caccagtaca gacaatgggt gtatgattca gaacagaatc
     8881 aataaagtgt aatatttccg ttctataagc ttcacgatag cgtagcgcaa aatcaggcaa
     8941 aggatttttc atgatacagt tctgactgtt atataccttc accgccgatt cactgacatt
     9001 gtcggcatac gccatccctg ctgaaccaaa gacctcaacc cgttggtcgt agccataaac
     9061 cgcccgacga ctattatcaa ttagcgcata accaccgttt ctgaaggtca gcatcaccac
     9121 gagagtatct acgtcatcaa tatttttcag agtaggatca atcaatgtat tgcctttaac
     9181 ataaacctcg ctaacttcat cctgcataat aaagcgagcc atatcaaagt catgcatagt
     9241 aaaatcaaat atgagtccac ctatcctctt gatcaaatcg tgcggcaaca actctggatc
     9301 acgtgaggtg atctttatca tctgaggctg accaatcttg cctctgcgca ccagttcata
     9361 cacctcccga aactggggat caaaacgacg attaaaagca atctgcagtt tgactccttt
     9421 ctctttaacc gcccttagca ccgcaaggga agctttctca tcttctctca tgctgagagg
     9481 tttttcgcag aaaatatgtt tacccgcctg gactgctgcc gtaacgatct cttcgtgcat
     9541 atctgtcgag gtgaaaataa acaccgcctc aatatcatca cgctgtaaca tttcctgata
     9601 atctttatat cctgaggtta tatttaccga actcagccag tcgctcattt cctcaataaa
     9661 actgtcagca gcacagacaa tatctaattg aggaagtaaa tacatattct caacatgcat
     9721 tttccctacc cggccaagac caataatccc acatcgaagc ttttgcattt atacctcgtc
     9781 atatcatgat atagtaaata taaaacgaca aaagaaaatc ccctttatcc aacacggtac
     9841 gaaagacttt attttgtagt ttcaacgaac aaatactttt accgtaaaaa acgtcaggtt
     9901 gcagaaaggc gccctcctgc aaccttaaaa actgaattaa ttagtactgt cgcgcttgtc
     9961 caatatgctc atcaagcata cgagctactg cctggatgcg ttcgctacgc gacacgcgag
    10021 cgccaccaac acgccaccag ctcagatatt tatgcgccat tgttttaggc agtactttaa
    10081 tgtctatcaa cgtactcacc gtctgtttac gtgcatcctc cagagcttca agcagttgtc
    10141 caatgctggt gacgcgatag cttttacagc catatccttc ggcaatccgg gcaaaatcca
    10201 caggaatgag tcccccctcc tgacgaccgt tatcctgttt atggaaacga aactcggtaa
    10261 agaaactgtc cataccgtgt tccatttgaa gattatttat acaaccatta gtcatgttat
    10321 ccagcaaaat aacattaatt ttcttcccca tctggaccga agttaccagc tcagaatgca
    10381 gcatcataaa tgagccgtcg ccaacgaagc tatatacctc ccgctccggt tgcgccaact
    10441 tcgcccccaa tgcggcattg acctcatagc ccatacagga atagccatat tctacgtggt
    10501 aatcattatc gccacggctt tgccagatgc gctgcagatc gcctggcaga cttcccgccg
    10561 cagcaaccac cagcgcgttt tctggcagca ttcgatttag tactccaaga acgcgcgtct
    10621 gtgtcagcca tgaatgagta atttcattga actcttcaaa cactttttga gtatccatat
    10681 gatccacaat ttccggtttg aaatccttac cgctgtattc cgctgcatag acgcgatcca
    10741 cttccgccag atattgagaa cgaacacgat ggatcgtttt cccccattgt gatatgtaac
    10801 cttccggagc cagtcgttcg caaagctgcg tcagggcaac acgggcatca gcctgcaatt
    10861 gcactccatc cagtttgaag acgtcaaaac ggctgacgtt tatattcaga tagcgcacat
    10921 caggattctg gaaaatccat ttagaagagg ttgtgaaatc ggtataacgc gtacccacac
    10981 caataatcag atcggcctcc tgagccagca aattcgccgc cagcgatccg gtttcaccaa
    11041 tcccaccaac attcagcgga tgcgcagaac tgagcgcacc tttaccagcc tgcgtttcag
    11101 caatcggcag atgatattgt tcggcaaatc gcagcagttc ctgttcagac tgcgaatact
    11161 tcacaccccc accgcaaata ataattggtc tctggtggcg aagaataaga gcaactgcat
    11221 cattcagcat tagctctgtt ggcacgtggc gctcaagacg atagacccga cgtgcaaaaa
    11281 aatagtctgg ataatcccag gcttctccct gaacatcctg cggcaaacaa agcgtcacag
    11341 ctcccgtatc agccggatcg gtaagcaccc gcatcgcgct aatgcaggcg ctcattagct
    11401 gttccggacg gataatgcga tcccaatagc gactcactgc acgaaaagca tcattggtgc
    11461 tcagcgtcag gtcatgctcc tgttcaatct gctgcaacac aggatcgggt tgacgcgatg
    11521 catacacatc tcctggtagc agaagcaacg ggatacggtt cgccgtcgca gtcgccgccg
    11581 ccgtgatcat attagcggca ccaggcccta cggaggaggt gcaggcataa atctgctgac
    11641 gacgcatctg acgcgcaaat cctgttgcta tatgtgccat accctgttca ttgcgtcctt
    11701 gatgaacgct aagttgacca cactcttctt ccagagcctg ccctaatccc acgacattcc
    11761 catggccaaa aattgcgaaa atacctttta caaacttgat ttcgcgaccg tccacttcaa
    11821 tgtactgatt atcaagaaag cgcaccaaag cctgcgccat agtcaacctg attgttttca
    11881 tgcactccac ctatcttatc aaccgtttga ctgtcgttac ctacgtacgc actgctacag
    11941 ccccgggcat aaaacactca ccacaagaaa aaaattccgc aaacgcaatt atatgaaaca
    12001 tatttttcat ttgtgagaag aagatcaaaa aacgattaaa aatttcgaga atagctaaaa
    12061 tacgaacaaa gaagaatgaa cattatgctt ccaaaattaa ctatacttat caacaaatta
    12121 atagcttttt agttg
//
LOCUS       DAZGTJ010000121        11800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000121 DAZGTJ010000000
VERSION     DAZGTJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.121"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..319
                     /locus_tag="ACHK8E_004455"
     CDS             <1..319
                     /locus_tag="ACHK8E_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein acyltransferase"
                     /protein_id="HFP0346632.1"
                     /translation="KNNNTFKLHGLSYRIYICYEALFPEKYVHNGVVITQSDYIRLNN
                     GRGYKTTLVNGSLLAKFSVAPNTKLINVQNYGGTIVFNNDWEIDWDIYNKSKKEHFFV
                     VTL"
     gene            complement(467..1402)
                     /locus_tag="ACHK8E_004456"
                     /pseudo
     CDS             complement(467..1402)
                     /locus_tag="ACHK8E_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1746..2366)
                     /gene="entD"
                     /locus_tag="ACHK8E_004457"
     CDS             complement(1746..2366)
                     /gene="entD"
                     /locus_tag="ACHK8E_004457"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP0346633.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            complement(2520..3868)
                     /locus_tag="ACHK8E_004458"
     CDS             complement(join(2520..3388,3388..3868))
                     /locus_tag="ACHK8E_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0346634.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            complement(3978..6218)
                     /gene="fepA"
                     /locus_tag="ACHK8E_004459"
                     /pseudo
     CDS             complement(3978..6218)
                     /gene="fepA"
                     /locus_tag="ACHK8E_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            6461..7663
                     /gene="fes"
                     /locus_tag="ACHK8E_004460"
     CDS             6461..7663
                     /gene="fes"
                     /locus_tag="ACHK8E_004460"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP0346635.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            7666..7884
                     /gene="ybdZ"
                     /locus_tag="ACHK8E_004461"
     CDS             7666..7884
                     /gene="ybdZ"
                     /locus_tag="ACHK8E_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP0346636.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            7881..11762
                     /gene="entF"
                     /locus_tag="ACHK8E_004462"
     CDS             7881..11762
                     /gene="entF"
                     /locus_tag="ACHK8E_004462"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP0346637.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT         2657 a         3078 c         3072 g         2993 t
ORIGIN      
        1 aaaaaataat aatacattta agcttcatgg cttaagttat agaatatata tatgctatga
       61 agcactgttt cctgaaaaat atgtacacaa cggggtcgta ataacacaat ctgattacat
      121 aagacttaat aacggtaggg gatataagac tacattggtt aacggtagct tacttgcaaa
      181 gttttccgtt gcaccaaaca ccaagttaat aaatgtccag aattatggtg gaactattgt
      241 ttttaataat gattgggaaa ttgactggga tatttataac aaatccaaaa aagaacattt
      301 ttttgtggtt actttataat acagcgaggg agtgttgttg aaatttttat gagctattta
      361 cgcgcccaca tattattcaa gtcttgcata tatatgctga gtagttaaat tcccctgtgc
      421 tatgccagtt actccattcg cttctaagta gctgatccta ataccgctac actttagcca
      481 gcccatgctt tccaccgggg aatgggctga cggtatccat aaagtgacga acctttttca
      541 acagttgcca cattgaacgg cactggtgat ttcgggttat tgtttcatga agcgcctgcc
      601 acaaccgttc gacatgattg acccatggcg agtaaattgg ctgataaatc accctgaact
      661 ttgggtttgc tttcagccag cgcaacgttt cacagctttt gtgggtgatg tagttatcga
      721 caatgagcgt aatctttttt gcccgtcagt atgtctcctt cagatgtttt aacagcctga
      781 taaataagga tgaaccttta ctgtttccac ccacgtagct gactttgccc gtaccactgt
      841 gcagcgcacc ggcaagatag tacttttcat tccggcccgg cgtaacaacg cgcttttgct
      901 gcccgcgtaa ctgccagtct gcgccgattt tgggattgag gtggatctcc acttcatctt
      961 cgtaaaacac agggtgttct atgctacaac tgactaacgc tacattaatc gctgccattt
     1021 tttcttcctt attcgggccc cggatacgca gtgttggtgc tgctctgcgc cataccagtt
     1081 cggctgcagg taaccatcgc cgggagaatg cttaacaagc tcacgcagca atgcgcaaat
     1141 atgctcgaac ggccagcgcc tctcacgtcc tgacggcaat gacttcaggc cttcaacacc
     1201 cgacagcgtg aaccagttaa tccagcgacc aatcgatgag cgagcacagc agagtgttct
     1261 ggcagcatga ctgacggtgt caccacgatg cagcatcagc atggctgtga gccgccgggc
     1321 gtggtttttt gtcctttgtt ttatggatcg ttttctggat caggcgtcgt tcagttctgg
     1381 gtattggtgc tatgatcggc actgctccgt ccggttggtg atttgggata ttcagcgatt
     1441 gatcagatcg cataatcgac tctgtaacaa cctgaacggt gtcggggtgg taacataggt
     1501 taggaaacag ggtgcggatg ccatcatggc gcgggtcatc agtccactaa cgacgccgga
     1561 tatatggcag caaatcagtg gttgtatatt gaacaactat aatagcgtca gatctcacca
     1621 ttataacggt gggctgacac cgaaaaaatt agagaacaga taacgttctt agcgtaaaac
     1681 ggtggtcaat attacttgac tactacaagc aatggttgct ggctaaccac agtaagggtt
     1741 atccattaat cgtgttgaca aagcgttatg agttgttttc ctcttatctg ccattgcaca
     1801 gcaaatatct cgttctccca acaaataatg atccgctgtt tattccagtt cactatctga
     1861 taatccagaa aaccttgatc tgtctgtaac ttgcttgcct taaacgcact ctctttcgct
     1921 gaaaacgcca gtgtaagcac cacagaaaag ggtaaattgc tgaccacaag cagctcgtat
     1981 tctgctggtg tcataatgct gtctttcaac tcttccgctg cttgtgcaga gaagatctct
     2041 tcgatatcaa tacccgttgg ttgatgggat accaccgcta atgccgtagc ctggcagtga
     2101 ctaatactgc cgtataaccc ggcaggccaa agtggctggc gacgttcacc gacagggggg
     2161 gcgtacttaa agccaaattc ccgtaaagca tgaacagcag caatccgtcc ggctaaatgt
     2221 tcggctttgc gtttccgtcc ggcatgttgc agttgcgcat aatgcggcag ccagagtaaa
     2281 tcctgttcgt gaaaacttgc tggctcgaac tcaacaaaat gcagcgtatg tccggcaaag
     2341 gggagggagg tatgcgtagt tttcatatcg accatattag agactgatga caaacgcaaa
     2401 actgcctgat gcgctacgct tatcaggcct acactatcct gcaatatatt gaattggcaa
     2461 gatttttgta ggccggataa ggcgttctga actgaacccc aaaacctgga cagtttcagt
     2521 taaccggctt tttgatactc agtccggtat tctaacgggc tgagtccccc aagactgagt
     2581 tttattcttt tttgattcca gtaagttatg tattcgttaa cggcattttc cagctcttct
     2641 acacttctgt agtcccggcg atagtacatt tcttctttca ggtgacctaa gaaattctcc
     2701 attaccgcat tatcaagaca gttgcccttg cgtgacatgc tctgcactaa ccctttgtct
     2761 gccagggcag actgataact ttttatccgg taatgccacc cttgatcatt gtggagcagc
     2821 ggtttttctc cttctgcaag gctttccagc cccttattta acatgcgctt caccagctcc
     2881 tctgtaggcc tgcaggcagt ttcccacgcc acgatttccc cgttaaacaa atcaagtatc
     2941 ggggacaggt acagtttttg tccgccagcc ctgaattcgg tgatatctgt cacccatttc
     3001 tcacagggag cttccgcttt gaactgccgc tgaaggatat tttctgcagc gagtcccata
     3061 tttccccggt aagaccggta tttcttcagg cgcacaggag acttcaggcc gagctgttgc
     3121 atcagttttc gcaccgtttt gccactgaac ttaagacctc ttttctgaag ctcacaatgg
     3181 atccgccggt acccataaca tccccggtgt tcatggaaga tggaggctat gagctgtttc
     3241 acatcagcat acttgtcctt agccttctgc agtgacagct ggtagtacag cgtgcttctg
     3301 gccagccctg cagcctttaa caggtcagac tggcagtgtc cgcacttcag gctccggatg
     3361 actttcgttt tttctccagc tcctggcgct ttttttccag gagatgctcc tgcatggctt
     3421 tcagatatgc attttctgcg cgaagataac gcagctcatt cttcatctct tccggcgtca
     3481 tgtttgcctc gttttcatgg gtatcatcag aacgggtcat gcttttctcc ttcctgcgcc
     3541 tgataaaggc ctcattacca gatttgcggt agccttttat ccagttttta accatgttgt
     3601 ggctgggaat gttaaaccgg gcagctaccc ggggtagaga ctcagaatgc ccgagagcat
     3661 aaagaaccac gcaaagttta tcttcaggag aatagctacg cttgtgtctg caatccagtg
     3721 cccgaggacc gtgaagaaga aaaagattaa tccagtgtga gaggctggtg tgagaaagtt
     3781 gaaagagttt tgccgtagaa atgatgcctg catgaccagc caggtaatga tttacagcgg
     3841 caagttttac ttcaaatgaa tgtttcataa actgcacctt cagatgtcag attatgtcca
     3901 acatttgggg tgcagttcag tttcacgccg catccggcat gaattacgcg cactttgtca
     3961 acaatctgac gttagcatca gaagtgagta ttaatgctca tataccacgt acgtcccggc
     4021 tcgttatagg tatacgcccc ggcaccggcg atatagttgg ctcctgccag atcgcccgtg
     4081 gtctgggcat tacccgcacg ccacaaacgt ttgtcgaaca ggttgtccac gccgccgatc
     4141 agactgacat tcttcgtcac atcccaggtc gcgctcagac ccacaatgct gtaaggactg
     4201 atttctttgg tttccggtcc aaccgctggc tgacctttat agttgtactt cttcggctgc
     4261 tgcttgccgt accaggtgaa ggttgtttgc atcgacaaat cttcccgtgc ctgccagctc
     4321 agcgttgagt tcaacgtata ctccgggatg atcgacaaac ggtcgcccgt ggttttgttt
     4381 tcactcttca gcatataagt gatgttattg gtccacatca ccgtttcgct aaccggtacg
     4441 tttaacgatc cttccagacc ttcaaccacc gctttcggta cgttatccca ctgatagaga
     4501 tcggtgccga ctgcgttttg ccctacagcc acatagcccg cttcaatctt attgcgataa
     4561 tcgttacgga accaggtgac gcccgccagc caaccgtcgc gtttgaactc cagaccaatc
     4621 tctttgttga tgctggtttc cgctttcagg tcatcattac cttgcagata gcagccgccc
     4681 gcgctggcat agcagccctg acctttactg tagagaatgt agtttgggtt agtctggtac
     4741 agactcggcg ctttataagc gcgagcaatg cccattttca gcgtgaagtc atcgcctaaa
     4801 ccttgcgata tgttcagcgc cggactccag ttattgccga caatactgtg atgatcgaaa
     4861 cgcagccccg gcgttacgat ggtgctgtca gttagctcca tattgttttc ggcaaacagc
     4921 gagaaaattt ctgcttttga atacgggcta cggtcggtgg cactcacgcc atcaatagcg
     4981 ccgccggtat tcgtcccggt gagcgcttgg gtgttggaac tcaagtcctt catccgctgc
     5041 tgattccact ccgtacccag cgtcagcgtc tggttaacga ggaaatcaat cggcaggtta
     5101 acttcgctgt gcagcatcac gtcatcgaga tcgatatcga cgaaatcctg tgtcgctttt
     5161 tcgttaaatt tcccttcggt accgcccgcc agaccttccg gaatacgcga gttacgggtg
     5221 tgttcgtact gcacccagtt gctggtggtc acgccgttat cccagccgcc gttccatgtc
     5281 agcgagtagt tctggcgata cagacggttg gtttcatcgc catatttcga gcgggtatag
     5341 gagtcagagt tggtgttctg ggtatcgccc gcatacaggt taccctggcg gctgtaaccc
     5401 gcttccagtt ccagcgattg caatggtgcg aagtcccagc gcaccacgcc gttaatatct
     5461 ttgttgatca ccccttcgcg cccggctggt aacgtcgtgg catacgttcc ggcacgcgcg
     5521 gactgatggc cctggttgat atcccacgcg tcagcctgag ttttgtcgag gttgccatac
     5581 aagcggaagc tgaattcgtc gcccagcgga ccggtgaggc taaagttagt gcgtttggtg
     5641 gcaccttcct ctttatgttc tggcgcattg aaataagcgt cccacgagcc gtgctactca
     5701 ccactgcctt ttttggtaat gatgttaacc acgccgcccg ccgcgccgtt gccataacgc
     5761 gcagctgccg gaccacgcag aacttcaata cgttcaatca tttcaggtgg cacccaggag
     5821 gtatcaccac gggtatcgcg ctcgccacgc cagccctgac gcaccgagtt acggctgctt
     5881 accggcttgc cgtcaatcaa aatcagcgtg ttttccggac ccataccgcg aatatcaatc
     5941 tggcggttat tcccacgctg accactggtg gagttaccgg tcaggttaac gcctggcatg
     6001 gtacggatga tctccgacac atcgcgggca accgggtttt tgcggatttc atctgcggtg
     6061 atggtcgaaa cgccaggtgc ctgcaagttc tgctcggcgg cggtaacgac aatggtatcg
     6121 tcatgtgaaa caggagtatc ggtcggctct tgtgcctgcg ctaccccata aatccccaga
     6181 ttgaccaaca aggccaggga atgaatcttc ttgttcattg ttttattcct gcatttttgc
     6241 cacgaattgc aactgtcggg catggtcgtc atcaacacga cgcatcccgc taccgcgaaa
     6301 acctttgatc ctgaaagaca cgcagtgcag ttggttaatt aatgtccgcg cttcccacgg
     6361 cgcgccatta cgctattgca aatgcaaata gttatcaata atattatcaa tatatttctg
     6421 caatcaatga aaaattgcac agtaaacatg gggttatggt gtgacggcgt taaaagtagg
     6481 aagtgagagc tggtggcagt cgaaacatgg cccggaatgg cagcgtctga atgacgaaat
     6541 gtttgaggtc actttccggt ggcgtgatcc ccagggttct gaagaatact cgacgataaa
     6601 gcgcgtatgg gtctacatca ctggtgtgac cgatcaccat cagaacagcc agccccagtc
     6661 gatgcagcga attgcaggca ccaacgtctg gcagtggacg acacaactta atgccaactg
     6721 gcgcggcagc tactgtttta ttcccaccga acgcgatgac attttttctg caccatcccc
     6781 cgatcgcctc gaattacgtg aaggctggcg aaaactatta ccccaggcga tagccgatcc
     6841 gctgaatcca caaagctgga aaggcgggcg agggcacgct gtttctgcac tcgaaatgcc
     6901 gcaagcgccc ctacaaccgg gatgggattg tccgcaagcg ccagaaacac ctgccaaaga
     6961 aattatctgg aaaagtgagc ggttgaaaaa ttcacgccgt gtatggattt ttaccactgg
     7021 cgatgcaaca gcaggagaac gtccgctggc agttttgctc gatggcgaat tttgggcgca
     7081 gagtatgcct gtctggcctg tgctgacttc gttgacccat cgtcagcaac ttcctccagc
     7141 cgtgtatgtg ttgatcgacg ctattgacac cacgcaccgc gcccacgaac tgccgtgtaa
     7201 tgcggatttc tggctggcag tacagcaaga gttattaccc ctggtgaaag ctattgcccc
     7261 ttttagcgat cgcgccgatc gcaccgtggt cgccgggcag agttttggtg ggctttccgc
     7321 gctgtatgcc ggactgcact ggcctgaacg ctttggctgt gtattaagcc agtcaggatc
     7381 gtactggtgg ccgcatcggg gcgggcagca agaaggtgtt ttacttgagc aacttaaagc
     7441 tggtcaagtt agtgccgaag gtctgcgcat tgtgctggaa gcgggtattc gcgagccgat
     7501 gatcatgcag gccaatcagg cgctgtatgc gcaattacac cccataaaag aatccatttt
     7561 ctggcgtcag gttgacggcg gacatgatgc gctttgttgg cgcggtggct tgatgcaggg
     7621 gctaatcgac ctctggcaac cacttttcca tgacaggagt tgaatatggc attcagtaat
     7681 cccttcgatg atccgcaggg agcgttttac attttgcgca atgcgcaggg gcaattcagt
     7741 ctgtggccgc aacaatgcgc cttaccggca ggctgggacg ttgtgtgtca gccgcagtca
     7801 caggtgtcct gccagcagtg gctggaagcc cactggcgta ctctgacacc ggcgaatttc
     7861 acccagttgc aggaggcaca atgagccagc atttaccttt ggtcgccgca cagcccggca
     7921 tctggatggc agaaaaactg tcggatttac cctccgcctg gagcgtggcg cattacgttg
     7981 agttaaccgg agaggttgat gcgccattac tggcccgcgc ggtggttgcc ggattagcgc
     8041 aagcagatac gctgcggatg cgttttacgg aagataacgg cgaagtctgg cagtgggtcg
     8101 atgatgcgct gacgttcgaa ctgccagaaa ctatcgacct gcgaaccaac attgatccgc
     8161 acggtactgc gcagtcatta atgcaggcgg atttgcaaca agatctgcgc gtcgatagcg
     8221 gtaaaccact ggtctttcat cagttgatac aggtggcgga taaccgctgg tactggtatc
     8281 agcgttatca ccatttgctg gtcgatggct tcagtttccc ggccattacc cgccagatcg
     8341 ccaatattta ctgcgcatgg ctgcgtggcg aaccaacgcc tgcttcgccg tttacgcctt
     8401 tcgctgatgt agtggaagag taccagcaat accgcgaaag cgaagcctgg cagcgtgatg
     8461 cggcattctg ggcggaacag cgtcgtcaac tgccaccgcc cgcgtcactt tctccggcac
     8521 ctttaccggg gcgcagcgct tcggcagata ttttgcgcct gaaactggaa tttaccgacg
     8581 gggaattccg ccagctggct acgcaacttt caggtgtgca gcgtaccgat ttagcccttg
     8641 cgctgacggc tttgtggctg gggcgattgt gcaaccgcat ggactacgct gccggattta
     8701 tctttatgcg tcgactgggc tcggcggcgc tgacggctac cggacccgtg ctcaacgttt
     8761 taccgttggg tattcacatt gcggcgcaag aaacgctgcc gcaactggca acccgactgg
     8821 cagcacaact gaaaaaaatg cgccgccatc aacgctacga cgccgaacaa attgtccgtg
     8881 acagtgggcg agcggcagga gaggaaccgc tgttcggtcc ggtactcaat atcaaggtat
     8941 ttgattacca actggatatt cctggtgttc aggcgcaaac ccataccctg gcaaccggtc
     9001 cggttaatga ccttgaactg gccctgttcc cggatgaaca cggagatttg agtattgaaa
     9061 tcctcgccaa taaacagcgt tacgctgagc caacgttaat ccagcatgct gaacgcctga
     9121 aaatgctgat tgcccagttc gccgcggatc ctgcgctgtt gtgcggcgat gtcgatatta
     9181 tgctgccagg tgaatatgcg cagctggcgc agatcaacgc cactcaggtt gagattccag
     9241 aaaccacgct gagcgcgctg gtggcagaac aagcggcaaa aacaccgcat gctccggcgc
     9301 tggcagatgc gcgttacctg ttcagctatc gggaaatgcg cgagcaggtg gtggcgctgg
     9361 cgaatctgct gcgtgagcgc ggcgttaaac caggcgacag cgtggcggtg gcgctaccgc
     9421 gctcggtctt tttgactctg gcgctacatg caattgttga ggctggtgcg gcctggttac
     9481 cgctggatac cggctatccg gacgatcgcc tgaaaatgat gctggaagat gcgcgtccgt
     9541 cgctgttaat caccaccgac gatcaactgc cgcgctttgc cgatgttcca gatttaacca
     9601 gcctttgcta taacgccccg cttacaccgc agggcagtgc gccgctgaaa ctttcacaac
     9661 cgcaccacac ggcttatatc atctttacct ctggctccac cggcaggccg aaaggggtaa
     9721 tggtcgggca gacggctatc gttaaccgtc tgctttggat gcaaaaccac tacccgctta
     9781 caggcgaaga tgtcgttgcc caaaaaacgc cgtgcagttt tgatgtctcg gtgtgggagt
     9841 ttttctggcc gtttattgcc ggggctaaac tggtgatggc tgaaccggaa gcgcaccgcg
     9901 acccgctcgc tatgcagcaa ttctttgccg aatatggtgt aacgaccacg cactttgtgc
     9961 cgtcgatgct ggcggcattt gttgcatcgc tgactccgca aaccgctcgc cagaattgcg
    10021 cgacgttgaa acaggttttc tgtagtggtg aggccttacc cgctgattta tgccgcgaat
    10081 ggcaacagtt aaccagcgtg ccgttgcata atctatatgg cccgacggaa gcggcggtag
    10141 atgtcagctg gtatccggct tttggcgagg aactggcaca ggtgcgcggc agcagtgtgc
    10201 cgattggtta tccggtgtgg aatacgggcc tgcgtattct tgatgcgatg atgcatccgg
    10261 tgccgccggg tgtggcggga gatctctatc tcaccggtat tcaactggcg caggggtatc
    10321 ttggacgacc cgatctgacc gccagccgct ttattgccga tccttttgcc cctggcgaac
    10381 ggatgtaccg taccggagac gttgcccgct ggctggataa cggcgcggtg gagtacctcg
    10441 ggcgcagtga cgatcagcta aaaattcgcg ggcagcgtat cgaactgggc gaaatcgatc
    10501 gcgtgatgca ggcgctgccg gatgtcgaac aagccgttac ccacgcctgt gtgattaacc
    10561 aggcggcagc cactggtggt gatgcgcgtc agttggtggg ctatctggtg tcgcaatcgg
    10621 gcctgccgtt ggataccagc gcattacagg ctcagcttcg cgaaacattg ccaccacata
    10681 tggtgcccgt ggttctgctg caacttccac agttacctct tagcgccaac ggcaagctgg
    10741 atcgcaaagc cttaccgttg ccggaactga aagcacaagc gccggggcgt gcgccgaaag
    10801 cgggcagtga aacgattatc gccgcggcat tctcgtcgtt gctgggttgt gacgtgcagg
    10861 atgccgatgc tgatttcttt gcgcttggcg gtcattcgct actggcaatg aaactggcag
    10921 cgcagttaag tcgacagttt gttcgtcagg tgacgccggg gcaggtgatg gtcgcgtcaa
    10981 ctgtcgccaa actggcaacg attattgatg gtgaagaaga cagcacccag cgcatgggat
    11041 tcgaaaccat tctgccgttg cgtgaaggta atggcccgac gctgttttgt ttccatccgg
    11101 cctccggttt tgcctggcag tttagcgtgc tctcgcgtta tctcgattca caatggtcga
    11161 ttatcggcat tcagtcgccg cgccctcatg gccccatgca gacggcggca aacctggatg
    11221 aagtctgcga agcgcatctg gcaacgttac ttgaacaaca accgcacggt ccttattacc
    11281 tgctgggtta ttcacttggc ggtacgctgg cgcagggcat tgcggcgcga ctgcgtgccc
    11341 gtggcgaaca ggtggcattt cttggcttgc tggatacctg gccgccagaa acgcaaaact
    11401 ggcaggaaaa agaagccaat ggtctggacc cggaagtgct ggcggagatt aaccgcgagc
    11461 gcgaggcctt cctggcggca cagcagggaa gtacttcaac ggagttgttt accaccattg
    11521 aaggcaacta cgctgatgct gtgcgcctgc tgacgactgc tcatagtgta ccgtttgacg
    11581 gtaaagcgac gctgtttgtt gctgaacgcg cgcttcagga aggtatgaat cccgaacgcg
    11641 cctggtcgcc gtggatagcc gagctggata tctatcgtca ggattgtgcg catgtggata
    11701 ttatctctcc aggggcgttt gaaaaaattg ggccgattat tcgcgctacg ctaaacaggt
    11761 aaattaatat tatctataaa cccataatta caggaaataa
//
LOCUS       DAZGTJ010000122        11644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000122 DAZGTJ010000000
VERSION     DAZGTJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.122"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..249
                     /locus_tag="ACHK8E_004463"
     CDS             <1..249
                     /locus_tag="ACHK8E_004463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346638.1"
                     /translation="IRECSSAAFHQDFRKIRNYASEFTDLIILILIYFEKGIGIYAVF
                     LKILIIGMDFRARKGFYMDNLLIISSSHHQQFTCLFRV"
     gene            342..2765
                     /gene="pgaA"
                     /locus_tag="ACHK8E_004464"
     CDS             342..2765
                     /gene="pgaA"
                     /locus_tag="ACHK8E_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP0346639.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2774..4792
                     /gene="pgaB"
                     /locus_tag="ACHK8E_004465"
     CDS             2774..4792
                     /gene="pgaB"
                     /locus_tag="ACHK8E_004465"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP0346640.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4785..6110
                     /gene="pgaC"
                     /locus_tag="ACHK8E_004466"
     CDS             4785..6110
                     /gene="pgaC"
                     /locus_tag="ACHK8E_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP0346641.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6112..6525
                     /gene="pgaD"
                     /locus_tag="ACHK8E_004467"
     CDS             6112..6525
                     /gene="pgaD"
                     /locus_tag="ACHK8E_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP0346642.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6575..7363)
                     /gene="phoH"
                     /locus_tag="ACHK8E_004468"
     CDS             complement(6575..7363)
                     /gene="phoH"
                     /locus_tag="ACHK8E_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP0346643.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7989..9260)
                     /gene="efeB"
                     /locus_tag="ACHK8E_004469"
     CDS             complement(7989..9260)
                     /gene="efeB"
                     /locus_tag="ACHK8E_004469"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP0346644.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9266..10393)
                     /gene="efeO"
                     /locus_tag="ACHK8E_004470"
     CDS             complement(9266..10393)
                     /gene="efeO"
                     /locus_tag="ACHK8E_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP0346645.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10451..11281)
                     /gene="efeU"
                     /locus_tag="ACHK8E_004471"
     CDS             complement(10451..11281)
                     /gene="efeU"
                     /locus_tag="ACHK8E_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP0346646.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3089 a         2852 c         2718 g         2985 t
ORIGIN      
        1 attcgtgagt gcagtagcgc tgcctttcat caggactttc gaaaaatccg aaattatgca
       61 tcggaattta ctgatttaat tattttaatc ctaatttatt ttgaaaaagg cattgggatt
      121 tatgccgtat tcctgaagat cctcatcatt ggaatggatt ttcgggcgag aaaaggattt
      181 tatatggaca atctgctcat catttcttct tctcatcatc aacaattcac gtgtctcttc
      241 cgcgtttaat aacggattat gagatgcaaa aatatctttc ttttcagtta cctgtaatta
      301 gatatagaga gagattttgg caatacatgg agtaatacag gatgtattca agtagcagaa
      361 aaaggtgccc gaaaaccaaa tgggctttga aacttcttac tgccgcattt ttagcagcga
      421 gtcccgcggc gaagagtgct gttaataacg cctatgatgc attgattatt gaagctcgca
      481 aaggaaatac tcagccagct ttgtcatggt ttgcacaaaa atcaacactc agtaataacc
      541 aaattgctga ctggttacaa attgccttat gggccgggca agataaacag gtcattaccg
      601 tttacagccg ctaccgtcat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg
      661 cttatcgtaa cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcgctctctc
      721 tggagccgca aaataaggat tatcaacggg gacaaatttt aaccctggca gatgcgggtc
      781 actatgatac tgcgctggtt aaacttaagc agcttaaatc tggagcgccg gacaaagcca
      841 atttactcgc agaagcctat atctataaac tgacggggcg tcatcaggat gaattacggg
      901 cgatgacaga gtcattacct gaaaatgcat ctaagcaaca atatcccaca gaatacgtgc
      961 aggcattacg taataatcaa ctagctgccg cgattgacga tgccaattta acgccagata
     1021 ttcgcgctga tattcatgcc gaactgatca gactgtcgtt tatgcctacg cgcagtgaaa
     1081 gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata cgctgcatta gaaattctgt
     1141 ggcacgataa ctcagaccgc actgcccagt accagcgtat tcaggttgat catcttggcg
     1201 cgttattaac tcgcgatcgt tataaagacg ttatttctca ctatcagcgt ttaaaaaata
     1261 cgaggcaaat tattccgccc tgggcgcaat attgggttgc gtcggcttat ctcaaagaac
     1321 agcagccaaa aaaagcacag tcaataatga cagagctctt ttatcacaag gagaccattg
     1381 ccccggattt atccgatgaa gaacttgcgg atctctttta cagccacctg gagagtgaaa
     1441 attatcccgg cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc
     1501 ggttaatggg cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc
     1561 tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc tgaaatgata gccagagagc
     1621 ttgcttataa cgcaccagga aatcagggac tgcgcattga ttacgcgagt gtgttacaag
     1681 cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa agcagaagtg atcgagccac
     1741 gtaatattaa tctggaggtt gaacaagcat ggacagcatt aacgttacaa gaatggcagc
     1801 aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac
     1861 gtttaaaacg tgcggttgat gtacataatc ttgtagagct tcgtatagct ggttcaacag
     1921 gaattgatgc cgaaggcccg gatagtggta aacatgatgt cgacttaacc accatcgttt
     1981 attcaccacc gctgaaggat aactggcgcg gttttgctgg attcggttat gccgatggac
     2041 aatttagcga aggaaaaggg attgttcgcg actggctggc gggtgttgag tggcggtcac
     2101 gtaatatctg gctcgaggcg gagtacgctg aacgcgtttt caatcatgag cataaacccg
     2161 gcgcgcgcct gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg
     2221 aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg
     2281 ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt
     2341 tcactgattt ttccgacagt aaccagcgtc aggaagtctc acttgagggt caggaacgca
     2401 tctggtcttc accatatttg attgtcgatt tcctacccaa tctgtattac gaacaaaata
     2461 cagaacacga cactccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg
     2521 aggcaagtca tttgttatgg cgaagctatg aaaatagctg ggagcaaatc ttcagcgcag
     2581 gtgttggtgc ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg
     2641 ggcaacgtat tagctggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac
     2701 gaccttatga cggtgacaga gaacacaact tatacgttga attcgatatg acattcagat
     2761 tttaaggata aatatgttac gtaatggaaa taaatatatc ctgatgctgg tgagtataat
     2821 tatgctcact gcgtgcatta gccagtcaag aacatcattt ataccgccac aggatcgcga
     2881 atctttactc gccgagcaac cgtggccgca taatggtttt gtggcgattt catggcataa
     2941 cgttgaagac gaagctgccg accagcgttt tatgtcagtg cggacatcag cactgcgtga
     3001 acaatttgcc tggctgcgcg agaacggtta tcaaccggtc agtattgctc aaattcgtga
     3061 agcacatcga ggaggaaaac cgctaccgga aaaagcagta gtgctgactt ttgatgacgg
     3121 ctaccagagt ttttataccc gcgtcttccc aattcttcag gccttccagt ggcctgctgt
     3181 atgggccccc gtcggcagtt gggtcgatac gccagcggat gaacaagtaa aatttggcga
     3241 tgagatggtc agtcgagaat attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc
     3301 ccggctcgtt gaggtcgctt ctcatacatg gaattctcac tacggtattc aggctaatgc
     3361 caccggcagc ttattgcctg tatatgtaaa tcgtgcatat tttactgacc acgcacggta
     3421 tgaaaccgca gcagaatacc gggaaagaat tcgtctggat gctgtaaaaa tgacggaata
     3481 cctgcgtaca aaggctgagg taaatccaca cgtttttgtt tggccttatg gcgaagcgaa
     3541 tggcatagca atcgcggaat taaaaaaact cggttatgac atgttcttca cccttgaatc
     3601 aggtttggca aatgcgtcgc aattagattc cattccgcga gtattaatcg ccaataatcc
     3661 ctcattaaaa gagtttgccc agcaaattat taccgtacag gaaaaaccac tacaacgggt
     3721 aatgcatatc gatcttgatt acatttatga cgaaaaccgc cagcaaatgg atcgcaatat
     3781 tgatgtgcta attcagaggg taaaagatat gcaaatatct accgtgtatt tacaagcatt
     3841 tgctgatccc gatggtgatg ggctggttaa agaggtctgg tttccaaatc gtttgctacc
     3901 aatgaaagca gatattttta gtcgggttgc ctggcaattg cgtacccgct caggtgtaaa
     3961 catctatgcg tggatgccgg tattaagctg ggatttagat cccacattaa cgcgagtaaa
     4021 atacttacca actggggaga aaaaagcaca aattcatcct gaacaatacc gccgtctctc
     4081 ccctttcgat gacagggtca gagcacaagt tggcatgtta tatgatgatc ttgccggaca
     4141 tgctgctttt gatggcatat tgttccacga tgatgctttg ctttcagatt atgaagatgc
     4201 cagtgcagcg gctatcgcgg cttatcagca agctggcttt agcgggagtc tgagcgaaat
     4261 tcgacaaaac ccggagcaat tcaaacagtg gacccgcttt aaaagtcgtg cgttaactga
     4321 cttcacttta gaacttagtg cgcgcgtaaa agccattcgt ggtccacatg ttaaaactgc
     4381 acgaaatatt tttgcacttc cggtaataca acctgaaagt gaagcttggt ttgcacagaa
     4441 ttatgctgat ttcctaaaaa gctatgactg gaccgctatt atggctatgc cttatatgga
     4501 aggcgtcaca gaaaaatcgg ctgatcaatg gttaacacaa ttgaccaatc agatgaaaag
     4561 catcccgcag gctaaagaca aatcaatttt agaattacag gcacaaaact ggcagaaaaa
     4621 tggtcagcat caggctattt cttcacaaca actcgctcac tggatgagcc tattacaact
     4681 gaatggagtg aaaaattatg gttattatcc cgacaatttt ctgcataacc aacctgaaat
     4741 agacgttatt cgtcctgagt tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg
     4801 tatcgttttt tatattatgt ctggtgttat gcatacctct atgcgtagcg tactttcact
     4861 ctggtgaact gatgatgagg ttcgttttct tctggccgtt ttttatgtcc attatgtgga
     4921 ttattggcgg cgtctatttc tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg
     4981 caccagcccc ccagttgaaa gataatccat ctatctccat tatcattccc tgttttaatg
     5041 aggagaaaaa cgttgaggaa accatacatg ccgctttagc acagcgttat gagaacattg
     5101 aagttattgc cgtaaatgat ggttcaacag acaaaacccg tgccatcctg gatcgcatgg
     5161 ctgcacaaat tccccgtctg cgggtcattc atctggcgca aaaccagggg aaagccattg
     5221 cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgacg
     5281 cgttattaga ccgcgatgca gcggcatata ttgtggaacc gatgttgtac aacccacgtg
     5341 tgggtgccgt aaccggtaat cctcgtattc gcacacgttc taccctagtg ggtaaaattc
     5401 aggttggcga gtattcctca attattggtt tgatcaagcg gacccagcgt atctatggaa
     5461 atgtatttac cgtttccggt gttattgccg catttcgtcg cagcgccctg gcagaagtgg
     5521 gttactggag tgacgatatg atcaccgaag atattgatat tagctggaag ctgcagttga
     5581 atcagtggac gattttttac gagccacggg cgctgtgctg gatattaatg cctgaaacgt
     5641 taaaagggct gtggaaacag cgcctgcgct gggctcaggg cggtgcagaa gtattcctca
     5701 aaaatatgac aaggttgtgg cgaaaagaaa actttcgaat gtggccactg ttttttgaat
     5761 actgcctgac gacaatatgg gcctttacct gcctggtcgg tttcattatt tacgcagtcc
     5821 aacttgcagg tgtaccgtta aatattgaat tgacacatat cgcagcgaca catactgccg
     5881 gaatattatt gtgcacgtta tgtttactgc aatttattgt cagtctggta attgagaatc
     5941 gctatgagca caatctggct tcatcgcttt tctggattat ttggtttccg gttattttct
     6001 ggatgctaag cctggcaacg acattggtat catttacacg agtcatgttg atgcctaaaa
     6061 aacaacgcgc tcgttgggta agtcccgatc gtgggattct gagaggttaa tatgaacaat
     6121 ctaattatta cgacccgaca atcgccagta cgtttactgg ttgattatgt tgccacaacc
     6181 atcttgtgga cattatttgc tttgttcata ttcttattcg ccattgatct gctgacgggt
     6241 tattactggc aaagcgaggc cagaagccga cttcagttct attttttgct ggcagtggcg
     6301 aatgccgtcg tgttaatcgt ctgggcattg tacaataagc tgcgttttaa aaaacagcag
     6361 catcatgcag cataccaata tacgccgcaa gaatatgcag agagcttagc aatacctgat
     6421 gagctttatc agcaactaca aaaaagccac aggatgagcg tacacttcac cagtcaggga
     6481 caaataaaaa tggtggtttc agaaaaaacg ctagtccggg tataaacacc caaaacaaag
     6541 cccggttcgc ccgggctctg caccgataat acacttaact gtatgcatgt agcgtacgtt
     6601 ggcaaagtgc cgaacgtacg caatcctctt taccgaatcg gatgatcccg accatttcat
     6661 cttcttcgaa acgttccagc gcgtcactta atccggagcg cacaccgcga ggtaaatcgc
     6721 attgcgtgat atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca
     6781 ttttcatttg cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa
     6841 aggtacgtcc acgcatatag gcgaacggcg cgatttcgac cttccctatt tctggtcgca
     6901 ggcagtactg cataaaggaa gcccctaagc gccggaccag cacgtcgtaa accgggcgaa
     6961 aatagggagc aaacttttct gcgatatctc caggtaaaaa gccaagatct tcatcggctt
     7021 gcagaactgg acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg
     7081 cttttgctgc gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct
     7141 gcttactctc aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg
     7201 aggtatcgcg actgtcgcgg gccataccaa tggcttctac gccgctcatt tgcacaagcg
     7261 aggtgaccga ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt
     7321 ttgcctcgcg acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt
     7381 gtttgtattc atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt
     7441 ggcttccttg taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac
     7501 cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa
     7561 gttgctaccg gaggagaaat ctccgcgagt ggtggagcct tgcttaacaa aacattgtcc
     7621 agaacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc
     7681 cgctgctact taataagtac aacagatctc gcattcattg caacaatata ttcacttata
     7741 tttaaccata aaacaccatt tcagtgacat tagtttctac tggaaagatg acaaagtgat
     7801 gacagtgata aaaaagctgt gtgctttcag caggattcgc agtactggta aaagcttgcc
     7861 ggatgcggcg tgaacgcctt atccggccta ccgaacccgc accgattgta ggcatgataa
     7921 gacgcgcaag cgtcgcatca gcgcacgaac gcctggtgcg gcgtaaacgc cttatccgcc
     7981 taaaaacatt aaacccgcaa taatgcgctt ccgagataat cgttcgcgtc cttcacaccc
     8041 ggcagcgcaa agaaatagcc gccaccgata ggtttgacat attcctccag cgcctctcca
     8101 ttcaaccttt tttgtactgt caggaagccc ttttccagat cgtgttggta gcagacaaac
     8161 agtagcccca tatcgagttg cccggaattg gtgacgccca acgaataact gtagccacga
     8221 cgcagcatca gacttgactc actctctggc gtgcggggat tcgccagccg gatatggctg
     8281 tccagcgcga tcaccttccc ctccggatcg ctggcgtaat caggcacatc atgctcatgc
     8341 tgcattccca gcggcgcgcc ggtttgctta tcacgcccaa atatcgtctg ctgttctttc
     8401 agcggcgttc tgtcccaaaa ttccacccga aactgaatca accgcaccgc ctggtagcta
     8461 ccaccgattg tccacgcagg ctcctgctga tctgccgtca cccacaccac tttttgcatc
     8521 aacttcgcat tctggctatc gggattggca gtgccgtctt tgaaacccag caaattaatc
     8581 ggtgtctctt tacctttact acgcgccgcg tggtcggaaa taaccccttc ccgcttccag
     8641 cgcacgctga gcaaatccgg cgtgtgtttg atgatatcgc gcagcgcatg gatcaccgtg
     8701 tcctgggtgt tggcgcaaat ttgtagcaac acatcaccat gacataacgc cgcatccagc
     8761 gagtcgttgg ggaaacgcgt catcttctgc aactttttcg gcatctgtgg cgcaaggcca
     8821 aagcgctcat caaacaatga gtggcccacc gataacgtta tggtgagatt atcgggcgca
     8881 atgtagccgc caagaatgcc ggaatcgagt ggtggcaggc gcggatttgg cgtttctggt
     8941 gccgctccgc cctgagtcag aaaagcaaaa cgctgagtca acaagcgaaa caaccgttca
     9001 agatcggctt tatcgctggc aagcacatca aacgccacca gcatcattgc agcctgttgt
     9061 ggcgtcagga tccctgcctg atgctcacca taaaacggct gtttctcatt gcgtgcatcc
     9121 ggtgaaagcg tacccggcgc actttgcgtt ttttgtgcat gagcgaccgg acaacttccc
     9181 gccagcgcca gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg
     9241 ttttcatctt catactgcat aacgcttagt ccagccccag cacaccgcga agttgcgcca
     9301 gatcttccgc cagcgcagta atcggtcctt tcagtgcatt ccggtcagca tcggtcagtt
     9361 tgtcgtaatt ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgaccttta
     9421 tgaagttggc atcgactttt gccaacagtt ccggattcac tttttgtagt tgtggacgca
     9481 gcagatcgac aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt
     9541 ggctgtagcg atcttcttca ccgctgattt tgctggccgc cacttcctca atcagtccgg
     9601 ctgcaccgcc gaccactttt gaaggaggga aagccagttc actgatgcgt ttttgcaaat
     9661 cgaccacatc ggtataaagc tggtcggcat acttatccat ccctttggtg gtgttatcgc
     9721 caaacaatgc tttttccaga cggtggaaac cggtgaattt cggatcggcg gctttttgct
     9781 cgtaatcatc ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg
     9841 gttcaatgcg ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat
     9901 cgcctgcttt aatagcatcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg
     9961 ccatgacata cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt
    10021 gcgccgcatc cgccgttgcc tcacctttga cgatcaactt ccctttcggg tttgtcagca
    10081 gaccgcaggt catatcgtat tcaccaggct gtaaatttgc cgtcattttc tggctaaaac
    10141 caggggcgat attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca
    10201 gtgctttctg gctgtggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg
    10261 tcatcggttc gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt
    10321 tagccataaa agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga
    10381 agttaatggt catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc
    10441 gtcgtatttg ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg
    10501 ccaccagcgc cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggcgctt
    10561 cctgatagcc aaaaatccct tccatcagcg tgccaaacac cgagtgagtt gagagcaccg
    10621 cactcatatc gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc
    10681 gaatggcacc agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa
    10741 aaaatgcacc aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg
    10801 ctgtagcaag accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaacg
    10861 ccgccagcag gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca
    10921 tcaccagcgc ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgctcca
    10981 gttgtacttt gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca
    11041 ccacggcgat caccgccacg ataccttcaa acagttcctg ttctttttgc ggaaattcgc
    11101 cggtggtttc gttaatgaag atacccaagc ccaggcacaa cgcagcggca agcaacacgc
    11161 caatccacat cacaccaatc catcggcctc gctgggtacg cttaagatag ctggcaatca
    11221 aactgacaat cagcgcggct tcaagtcctt cgcgcaacat aatgagaaac ggaacaaaca
    11281 tgccacccac ccttaaacgt ttttctgagt caatctatgc aaagaaaagt aaatcgcagt
    11341 gatagcgatt atcattacgg taaagaaaaa tacaagcaga atgtttataa aatgatgttg
    11401 gaatgtaaac aaagaaaggt aatgccaggg gatataaacg attattccag gtacaaagat
    11461 gggattgttt acaacgagcc atgtgttctt gccagatgcc gcgtaaatgc cttatccagc
    11521 cgacaaaact gtccagactc aatgcatcag agcttccatg taaaactgat aagcgggtgt
    11581 atcaagcaat ttcgtccatc cgcgattatc aaatctaact ttttgcccgg aacataataa
    11641 cagc
//
LOCUS       DAZGTJ010000123        11568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000123 DAZGTJ010000000
VERSION     DAZGTJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.123"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            42..911
                     /locus_tag="ACHK8E_004472"
     CDS             42..911
                     /locus_tag="ACHK8E_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0346647.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(961..1314)
                     /locus_tag="ACHK8E_004473"
     CDS             complement(961..1314)
                     /locus_tag="ACHK8E_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0346648.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1452..3098)
                     /gene="groL"
                     /locus_tag="ACHK8E_004474"
     CDS             complement(1452..3098)
                     /gene="groL"
                     /locus_tag="ACHK8E_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0346649.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3142..3435)
                     /locus_tag="ACHK8E_004475"
     CDS             complement(3142..3435)
                     /locus_tag="ACHK8E_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0346650.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3711..4967
                     /gene="yjeH"
                     /locus_tag="ACHK8E_004476"
     CDS             3711..4967
                     /gene="yjeH"
                     /locus_tag="ACHK8E_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0346651.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4983..5459)
                     /locus_tag="ACHK8E_004477"
     CDS             complement(4983..5459)
                     /locus_tag="ACHK8E_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0346652.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5796..7232
                     /gene="aspA"
                     /locus_tag="ACHK8E_004478"
     CDS             5796..7232
                     /gene="aspA"
                     /locus_tag="ACHK8E_004478"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0346653.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7351..8652
                     /gene="dcuA"
                     /locus_tag="ACHK8E_004479"
     CDS             7351..8652
                     /gene="dcuA"
                     /locus_tag="ACHK8E_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0346654.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8768..9106
                     /gene="cutA"
                     /locus_tag="ACHK8E_004480"
     CDS             8768..9106
                     /gene="cutA"
                     /locus_tag="ACHK8E_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0346655.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9082..10779
                     /gene="dsbD"
                     /locus_tag="ACHK8E_004481"
     CDS             9082..10779
                     /gene="dsbD"
                     /locus_tag="ACHK8E_004481"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0346656.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10816..11391
                     /locus_tag="ACHK8E_004482"
     CDS             10816..11391
                     /locus_tag="ACHK8E_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0346657.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2762 a         3004 c         2861 g         2941 t
ORIGIN      
        1 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
       61 gtgttaatac cggtgttatt cgcaagagca ataatttcat tgcactggcg ctgaaaatta
      121 aagagcctcg caataaagaa tcgttgttct ttctgtctgt catggaactg cgcgaccttc
      181 tgattgcgct ggaaagccgt ctgcatcaaa agcataaact ggatgctgct gcacgccttc
      241 agtatgagca agctcgcgat aaagtcatta agaaaatggt agaaaatatc ccagaaattc
      301 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
      361 ataatcaggg cgaaaatctt accttcgtat taacactaca tgacggtagc aaatgtgagt
      421 tagtcatcaa tgaattacag attgaaatgc tggcgcgagc aatcattcat gcaattaata
      481 atgcggaaat gcgtgaactg gcattacgta ttacttcact gttagatttc ctgccgttat
      541 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
      601 ggaaacataa cttgttcaac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
      661 gcaaagaaca atttagcggc gcagtggtga aaacgcgtga agcaacgccg ggtaaagaag
      721 tggaggcgat cactcgccga atgcttgatt tcagcccccg actgaaaaaa ctcgcgggtg
      781 tgccatgtca ggtctatgtt cgcaccgtag cggcaaataa cgcccaaccg ctaacccagg
      841 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
      901 aggcgaaata atctcatctg ttttactacg aggctggaag tatccagcct ctggatcaca
      961 tcagttcggg cacttataaa cctgaccgat aatctggcta tccgtcggga caaaactgga
     1021 caacattccc tgcgacgggc tgctgatgcc ataaatcacg ttaccgccca ttgcagccgc
     1081 ctggttgcgc agatcgtttg ctgcgccgcg catagaaccg ccctcttctc cgtgttgccc
     1141 ggaaagccag ttgctttgct tacctgtcgc agtaccaatc agctggcact ctgcgcctgg
     1201 ctgctcgtcc acaatgcgta cactctgacc ggcagcactc aattcgttgc tggagctaca
     1261 acccgccatc agtagcgcgg caccgacaat ccctgctaag tattttacgt gcatgttatt
     1321 ccccataatc aatgagctgg acgccactcg cgtcgtccgt gtctgaatct tatactagaa
     1381 agatgaccaa aagaaaaacc cccagacatt tctgcccggg ggtttgttta tttctgcgag
     1441 gtgcagggca attacatcat gccgcccatg ccacccatgc cgcccatacc gccagcagcg
     1501 cctaagtcag ctgcatcgtt tttcggcagg tcggtaacca tgcattcggt ggtgatcatc
     1561 aggccagcca cagaagctgc gtactgcaga gcagaacgag ttactttggt tggatccagg
     1621 atacccatgt cgatcatgtt gccgtattct tcggttgctg cgttgtaacc gtagttgccg
     1681 tcgccgcctt taacggtgtt agcaacaaca gacggttctt cgccgcagtt caatacgatc
     1741 tgacgcagcg gagcttccat tgcacgcagt gcaactttga tacccacgtt ctggtcttcg
     1801 ttctgaccac gcaggtcagc cagtttagac gctacgcgga tcagcgcaac accaccacca
     1861 gcaaccacgc cttcttctac cgcagcacgg gtcgcgtgca gggcatcttc aacgcgtgct
     1921 tttttctctt tcatttcaac ttcggtagca gcacccactt tgataactgc aacgccgcct
     1981 gccagtttcg ctacgcgctc ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca
     2041 atctgctgac ggatctgagc aacacggccc tggattgcag cttcttcacc cacgccatca
     2101 atgatggtgg tggtgtcttt gttgatcaca acacgtttag cctgacccag gtcttccagg
     2161 gttgcttttt ccagctccat accgatctct tcagagatta cggtaccgcc agtcagggtt
     2221 gcgatatcct gcagcatagc tttacgacga tcgccgaagc ccggagcttt aaccgcagca
     2281 actttcacga tgccacgcat ggtgttaaca accagagttg ccagcgcttc gccttctaca
     2341 tcttcagcga tgatcagcag cggtttgcct gctttggcaa cagcttccag aaccggcagc
     2401 atttcgcgga tgttggagat tttcttgtca gccagcagga tgaacgggct ttccagttct
     2461 actgcgccag tttccggctt gttgataaag taaggagaca ggtagccacg gtcgaactgc
     2521 ataccttcaa ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg
     2581 ccttctttac cgactttgtc catcgcttca gcgatcagtt tacctacggt ttcgtcggag
     2641 ttagcggaga tggtaccaac ctgagcaatc gctttagagt cagagcatgg tacggacagc
     2701 gctttcagtt cttcaactgc agcggtaacc gctttgtcga taccacgttt caggtccatc
     2761 gggttcatgc ccgcagcaac agctttcaga ccttcagtga tgatagcctg agccagtacg
     2821 gttgcagtgg tggtaccgtc gcctgcagcg tcgttcgctt tggaggcaac ttctttcacc
     2881 atctgcgcac ccatgttttc gaacttgtct tccagttcga tttcacgagc aacggaaaca
     2941 ccatctttgg tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt acggcctttc
     3001 ggaccgaggg taactttcac tgcatctgcc agtacgttta cgccgcgcag cattttcaca
     3061 cgagcgtcgt taccgaattt tacgtcttta gctgccatta tctttattcc ttaaattcgt
     3121 atgttcagtg tcgtgcgcgg attacgcttc aacaattgcc agaatgtcgc tttcggacat
     3181 gatcaacact tcttcattgt cgatcttctc ggatttcaca ccgtagccat cgttgaaaat
     3241 aacgatgtcg ccaactttca cgtccagcgg cttcacttcg ccattttcaa ggatacggcc
     3301 attgccgaca gccagcactt cgccgcgggt ggatttagcc gctgcagagc cggtcagaac
     3361 gatgccgcca gcagatttag tttcaacttc tttacgcttg acgatcacgc gatcatgcaa
     3421 tggacgaata ttcattgata actctccttt gagaaagtcc gtatctgtta tgggtgacgc
     3481 cggagcttac gtggtttccc ggctggtgac cagagaaatg gggatgaggc ttcgcccctt
     3541 caagggggaa aaagaaaaaa aattctgatc agcacaaaat cgggtgaaaa ccctgattca
     3601 cctcacattt catcgcaatt tcttcatcgc cgtcataagc gaatctgatt gtgctaccat
     3661 cgaaaatcta cgcagttgcc caaaatttgg gcgcaatcgg acatcaaccc atgagtggac
     3721 tcaaacaaga actggggctg gcccagggca ttggcctgct atcgacgtca ttattaggca
     3781 ctggcgtgtt tgccgttcct gcgttagctg cactggtagc gggcaataac agcctgtggg
     3841 cgtggcccgt tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc
     3901 gccactatcc cagcgcaggc ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc
     3961 ttgagcgagt caccggctgg ctgtttttat cggtcattcc cgtgggtttg cctgccgcac
     4021 tacaaattgc cgccgggttc ggccaggcaa tgtttggctg gcatagctgg caactgttgt
     4081 tggcagaact cggtacgctg gcgttggtgt ggtatatcgg tactcgcggt gccagttcca
     4141 gtgcaaatct acaaaccgtt attgccggac ttatcgtcgc gctgattgtc gctatctggt
     4201 gggcgggcga tatcaaacct gcgaatatcc ccttcccggc acctggtaat atcgaactta
     4261 ccgggttatt tgctgcgtta tcagtgatgt tctggtgttt tgtcggtctg gaggcatttg
     4321 cccatctcgc ctcggaattt aaaaatccag agcgtgattt tcctcgtgct ttgatgattg
     4381 gtctgctgct ggcaggatta gtctactggg gatgtacggt agtcgtctta cacttcgacg
     4441 cctatggtga aaaaatggcg gcggcagcat cgcttccaaa aattgtagtg cagttgttcg
     4501 gtgtaggagc gttatggatt gcctgcgtga ttggctatct ggcctgcttt gccagtctca
     4561 acatttatat acagagcttc gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc
     4621 actacctgtc acgcctctct tctcgccata tcccgaataa tgccctcaat gcggtgctcg
     4681 gctgctgcgt ggtgagcact ttggtgattc atgctttaga gatcaatctg gacgctctta
     4741 ttatttatgc caatggcatc tttattatga tttatctgtt atgcatgctg gcaggctgta
     4801 aattattgca aggacgttat cgactactgg cggtggttgg cgggctgtta tgcgttctgt
     4861 tactgacaat ggtcggctgg aaaagtctct atgcgctgat catgctggcg gggttatggc
     4921 tgtttctgcc aaaacgaaaa acgccggaaa atggcataac cacataatcc ggcgtttcga
     4981 cattaatcct ggcgatcgtc tttatgatca aggcggtcgc ggtcatcatc ctttcgctgg
     5041 tactcaccat caaaagtatt accgccaccg gtcccggcgc taaaaccgcc gcctggcatg
     5101 cgagaaaagc gcaaatgcgg catcaacttc actgtcagat gcttttgcac cggcggcaac
     5161 aaaagcagaa gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag caaaccggca
     5221 atgatcagcg aaacactttt aatcatctcc gccgctgggt tttcacccgc cgccattttt
     5281 tgctgcatca gcacgaaatt cttaaagccc tggttacgta ccagtgacat accgataact
     5341 gacgtgaata taaccagcac gagagtcagc aataccccca atacatgggc aacctgaata
     5401 aagattgaaa tctcaatata gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa
     5461 ggtttctcct gtaatagcag ccggttaaac ccggctacct gaatgggttg cgaatcgcgt
     5521 tcagcttata ttgtggtcat tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag
     5581 taatcccaaa gcggtgatct atttcacaaa ttaataatta aggggtaaaa accgacactt
     5641 aaagtgatcc agattacggt agaaatcctc aagcagcata tgatctcggg tattcggtcg
     5701 atgcagggga taatcgtcgg tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa
     5761 agcaaatcat tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag
     5821 aagatctgtt gggtaccagg gaagttccag ctgatgccta ctatggtgtt cacactctga
     5881 gagcgattga aaacttctat atcagcaaca acaaaatcag tgatattcct gaatttgttc
     5941 gcggtatggt aatggttaaa aaagccgcag ctatggcaaa caaagagctg caaaccattc
     6001 ctaaaagtgt agcgaatgcc atcattgccg catgtgatga agtcctgaac aacggaaaat
     6061 gcatggatca gttcccggta gacgtctacc agggcggcgc aggtacttcc gtaaacatga
     6121 acaccaacga agtgctggcc aatatcggtc tggaactgat gggtcaccag aaaggtgaat
     6181 atcagtacct gaacccgaac gaccatgtta acaaatgtca gtccactaac gacgcctacc
     6241 cgaccggttt ccgtatcgca gtttactctt ccctgattaa gctggtagat gcgattaacc
     6301 aactgcgtga aggctttgaa cgtaaagctg tcgaattcca ggacatcctg aaaatgggtc
     6361 gtacccagct gcaggacgca gtaccgatga ccctcggtca ggaattccgc gctttcagca
     6421 tcctgctgaa agaagaagtg aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta
     6481 accttggcgc aacagcaatc ggtactggtc tgaacacgcc gaaagagtac tctccgctgg
     6541 cagtgaaaaa actggctgaa gtcactggct tcccatgcgt accggctgaa gacctgatcg
     6601 aagcgacctc tgactgcggc gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg
     6661 tgaagatgtc caaaatctgt aacgacctgc gcttgctctc ttcaggccca cgtgccggcc
     6721 tgaacgagat caacctgccg gaactgcagg cgggctcttc catcatgcca gctaaagtaa
     6781 acccggttgt tccggaagtg gttaaccagg tatgcttcaa agtcatcggt aacgacacca
     6841 ctgttaccat ggcagcagaa gcaggtcagc tgcagttgaa cgttatggag ccggtcattg
     6901 gccaggccat gttcgaatcc gttcacattc tgaccaacgc ttgctacaac ctgctggaaa
     6961 aatgcattaa cggcatcact gctaacaaag aagtgtgcga aggttacgtt tacaactcta
     7021 tcggtatcgt tacttacctg aacccgttca tcggtcacca caacggtgac atcgtgggta
     7081 aaatctgtgc cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga
     7141 ctgaagcgga acttgacgat attttctccg tacagaatct gatgcacccg gcttacaaag
     7201 caaaacgcta tactgatgaa agcgaacagt aatcgtacag ggtagtacaa ataaaaaagg
     7261 cacgtcagat gacgtgcctt ttttcttgtg agcagtaact taaaaataac aatctaatat
     7321 caacttgtta aaaaaacaag gaaggctaat atgctagttg tagaactcat catagttttg
     7381 ctggcgatct tcttgggcgc cagattgggg ggaataggta ttggttttgc aggcggattg
     7441 ggggtgctgg ttcttgccgc tattggcgtt aaacccggta acatcccgtt cgatgtcatt
     7501 tccattatca tggcggttat cgccgctatt tctgccatgc aggttgctgg cggtctggac
     7561 tatctggttc atcagacaga aaagctgctg cgccgtaacc cgaaatacat cacgatcctc
     7621 gcaccgatcg tgacctattt cctgacaatc tttgctggta ctggcaacat ctctctggcg
     7681 acactgccag ttatcgctga agttgcgaag gaacaaggcg ttaaaccttg ccgtccgctg
     7741 tctactgcag tggtatccgc gcagattgcg atcaccgcat cgccaatctc agcggcagtg
     7801 gtttacatgt cttccgtaat ggaaggtcat ggcatcagct acctccatct gctctccgtg
     7861 gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct tcctggtcac tatgctgttc
     7921 aactccaaac tctctgacga tccgatttat cgcaagcgtc tggaagaagg cctggttgaa
     7981 ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa aaacgtccgt ctggctgttc
     8041 ctgctgggcg tagttggcgt tgttatctat gcaatcatca acagcccaag catgggtctg
     8101 gttgaaaaac cgctgatgaa caccaccaac gcaatcctga tcatcatgct cagcgttgca
     8161 actctgacca ccgttatctg taaagtcgat accgacaaca tcctcaactc cagcaccttc
     8221 aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg cgtggctggg cgatactttc
     8281 gtttccaaca acatcgactg gatcaaagat accgctggtg aagtgattca gggtcatccg
     8341 tggctgctgg ccgtcatctt cttctttgct tctgctctgc tgtactctca ggctgcaacc
     8401 gcaaaagcac tgatgccgat ggctctggca ctgaacgttt ctccgctgac cgctgttgct
     8461 tctttcgctg cggtgtctgg tctgttcatt ctgccgacct acccgacgct ggttgctgcg
     8521 gtacagatgg atgacacggg tactacccgt atcggtaaat tcgtcttcaa ccatccgttc
     8581 ttcatcccgg gtactctggg tgttgccctg gccgtttgct tcggcttcgt gctgggtagc
     8641 ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg ctttctttcc cgccgactaa
     8701 catccttccc ccgtccgttg tatagtgacc tctctcttgc ggttccatct gttcttgcga
     8761 ggtgtttatg cttgatgaaa aaagttcgaa taccgcgtct gtcgtggtgc tatgtaccgc
     8821 accggatgaa gcgacagccc aggatttagc cgccaaagtg ctggcggaaa aactggcggc
     8881 ctgcgcgacc ttgatccccg gcgctacttc tctctattac tgggaaggta agctggagca
     8941 agaatacgaa gtgcagatga ttttaaaaac taccgtatct caccagcagg cactgctgga
     9001 atgcctgaag tctcatcatc catatcaaac cccggaactt ctggttttac ctgttacaca
     9061 cggagacaca gattacctct catggctcaa cgcatcttta cgctgatcct gctactttgc
     9121 agcacttccg tttttgccgg attattcgac gcgccgggac gttcacaatt tgtccccgcg
     9181 gatcaagcct ttgcttttga ttttcagcaa aaccaacatg acctgaatct gacctggcag
     9241 atcaaagacg gttattacct ctaccgtaaa cagatccgca ttacgccgga acacgcgaaa
     9301 attgccaacg tgcagctgcc acaaggcgtc tggcatgaag atgagtttta cggcaaaagc
     9361 gagatttacc gcgatcggct gacgcttccc gtaaccatca accaggcgag tgcgggagcg
     9421 acgttaactg tcacctacca gggctgtgct gatgccggtt tctgttatcc gccagaaacc
     9481 aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag cgtcacagcc tgtgtctgtt
     9541 ccgcagcaag agcagcccac cgcgcaattg cccttttccg cgctctgggc gttgttgatc
     9601 ggtattggta tcgcctttac gccatgcgtg ctgccaatgt acccactgat ttctggtatc
     9661 gtgctgggtg gtaaacagcg gctctccact gccagagcat tgttgctgac ctttatttat
     9721 gtgcagggga tggcactgac ttacacggcg ctgggtctgg tagttgccgc cgcagggtta
     9781 cagttccagg cggcgctaca gcacccatac gtgctcattg gcctcgccat cgtctttacc
     9841 ttgctggcga tgtcaatgtt tggtttgttt accctgcaac tcccctcttc gctgcaaacg
     9901 cgcctcacgc tgatgagcaa tcgccaacag ggcggctcac ctggcggtgt gtttgttatg
     9961 ggagcgattg ccggactgat ctgttcacca tgcaccaccg caccgcttag cgcgattctg
    10021 ttgtatatcg cccaaagcgg gaacatgtgg ctggggggcg gcacgcttta tctctatgcg
    10081 ttgggcatgg gcctgccgct gatgctaata accgtctttg gtaaccgcct gctgccgaaa
    10141 agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt ttgtgatcct cgcactgccg
    10201 gtcttcctgc tggagcgagt gattggcgat atatggggat tacgcttgtg gtcggcgctt
    10261 ggtgtcgcat tctttggctg ggcctttatc accagcctac aggccaaacg cggctggatg
    10321 cgcgtggtgc aaataatcct gctggcagcg gcattggtta gcgtgcgccc acttcaggat
    10381 tgggcatttg gtgcaacaca taccgcgcaa actcagacgc atctcaactt tacacaaatc
    10441 aaaacggtag atgagttaaa tcaggcgctc gttgaagcca aaggcaaacc agtgatgtta
    10501 gatctctatg ccgactggtg cgtcgcctgt aaagagtttg agaaatacac cttcagcgac
    10561 ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc aggctaacgt aactgccaac
    10621 gacgcacaag atgtggcgct gttaaagcat cttaatgtcc ttggcctacc gacaattctc
    10681 ttttttgacg gacaaggcca ggagcatcca caagcacgcg tcacgggctt tatggatgct
    10741 gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa cgacacttgc agtgggaaag
    10801 acggaggaaa atagcgtgca acgtgaagat gtactgggag aagccctgaa attattagaa
    10861 ttacaaggga ttgccaacac cacgcttgag atggttgctg aacgtgtgga ttacccactg
    10921 gacgagctac gccgcttctg gccagacaaa gaggcaatcc tctacgatgc gctgcgttat
    10981 cttagccaac agatagatgc ctggcgtcgt cagctgatgc tggacgaaac gcaaaccgcc
    11041 gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt gcgttaaaaa caaccgctat
    11101 ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg atcctggcca ccctattcat
    11161 caactggccg atcagcaaaa aagcgcggcc tacgatttca cccacgaact gttaaccaca
    11221 ctggaagttg acgatccggc gatggtagca aagcagatgg aactggtgct ggaaggctgt
    11281 ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg acaccgcaca tcggctggcg
    11341 gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg ctttgacctg acagaaacac
    11401 agaaaagaag cgatttgccg caatcttaag cagttgaatc gcttttactg aaattaggtt
    11461 gacgagatgt gcagattacg gtttaatgcg ccccgttgcc cggatagctc agtcggtaga
    11521 gcaggggatt gaaaatcccc gtgtcggtgg ttcgattccg cctccggg
//
LOCUS       DAZGTJ010000124        11499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000124 DAZGTJ010000000
VERSION     DAZGTJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.124"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            252..551
                     /gene="yoaC"
                     /locus_tag="ACHK8E_004483"
     CDS             252..551
                     /gene="yoaC"
                     /locus_tag="ACHK8E_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP0346658.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(671..850)
                     /gene="yoaH"
                     /locus_tag="ACHK8E_004484"
     CDS             complement(671..850)
                     /gene="yoaH"
                     /locus_tag="ACHK8E_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP0346659.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            924..2285
                     /gene="pabB"
                     /locus_tag="ACHK8E_004485"
     CDS             924..2285
                     /gene="pabB"
                     /locus_tag="ACHK8E_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP0346660.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2288..2866
                     /gene="nudL"
                     /locus_tag="ACHK8E_004486"
     CDS             2288..2866
                     /gene="nudL"
                     /locus_tag="ACHK8E_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP0346661.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3050..4414
                     /gene="sdaA"
                     /locus_tag="ACHK8E_004487"
     CDS             3050..4414
                     /gene="sdaA"
                     /locus_tag="ACHK8E_004487"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0346662.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4545..6143
                     /gene="pdeD"
                     /locus_tag="ACHK8E_004488"
     CDS             4545..6143
                     /gene="pdeD"
                     /locus_tag="ACHK8E_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0346663.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6147..7703)
                     /gene="yoaE"
                     /locus_tag="ACHK8E_004489"
     CDS             complement(6147..7703)
                     /gene="yoaE"
                     /locus_tag="ACHK8E_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP0346664.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7691..7849)
                     /gene="yoaL"
                     /locus_tag="ACHK8E_004490"
     CDS             complement(7691..7849)
                     /gene="yoaL"
                     /locus_tag="ACHK8E_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP0346665.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8166..9137
                     /gene="manX"
                     /locus_tag="ACHK8E_004491"
     CDS             8166..9137
                     /gene="manX"
                     /locus_tag="ACHK8E_004491"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP0346666.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9200..10000
                     /gene="manY"
                     /locus_tag="ACHK8E_004492"
     CDS             9200..10000
                     /gene="manY"
                     /locus_tag="ACHK8E_004492"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP0346667.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10013..10864
                     /gene="manZ"
                     /locus_tag="ACHK8E_004493"
     CDS             10013..10864
                     /gene="manZ"
                     /locus_tag="ACHK8E_004493"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP0346668.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10919..11377
                     /gene="yobD"
                     /locus_tag="ACHK8E_004494"
     CDS             10919..11377
                     /gene="yobD"
                     /locus_tag="ACHK8E_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP0346669.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2822 a         2894 c         2869 g         2914 t
ORIGIN      
        1 tgccgttatg ttcgcccttc gattagcatc tctggttttc tttcttacct gaacaatctg
       61 gttatagcgt gaggactgac cattaatttt ctccatctta ctggataaca cactatgagc
      121 tgctaccttt aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc
      181 tttgatatta gatacaaatt aaggtcatat atagccttat cgataacatg gttaatttta
      241 aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg
      301 tatcagcgcc aacaccaagt tcgatgaatg aaagtacggc gaagggaatc tttaaatatt
      361 taaaagaact gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag
      421 gctggaaccc tggtttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagtctggtg
      481 aacgctctgt gattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag
      541 cactggtttg agttaaattt acatcagcat aaatgggtca ggacgctttt aatcacatat
      601 aaaaagcgtc ctttttctca caatcaaata atcatcggct tctataacga aagcgatacg
      661 gattagtgtg ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggcatggt
      721 tggcacgcag ctcttctgcc accaacgcga ttgcctggcc gctgctcatc ccttgagcca
      781 tcagttcctg gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac
      841 ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca
      901 catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct
      961 ggcgtcagga cgccgctgaa ttttatttct cacgcttaag ccacctgccg tgggcgatgc
     1021 ttttacactc cggctatgct gatcatccat atagccgctt tgatattgtg gtcgccgagc
     1081 cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaaac gaaaaacgca
     1141 caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc
     1201 gcccagcgca taacgaagat ttgccatttc agggcggcgc gctggggttg tttggctacg
     1261 atctgggccg ccgttttgag tcactgccag aaattgcgca gcaagatatc gttctgccgg
     1321 atatggcagt gggtatctac gactgggcgc tggttgttga ccaccagcgt caaacagttt
     1381 ctttgctgag tcataatgat gtcaatgctc gtcgggcctg gctggaaagc cagcaattct
     1441 cgccgcagga aggtttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt
     1501 acggcgaaaa atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga
     1561 atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc
     1621 ttaatcaggc caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt
     1681 taagcctttc gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga
     1741 ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac
     1801 tggcgaactc agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg
     1861 atatcggtcg cgttgccgta gccggttcgg taaaagtacc agagctgttc gtggtcgaac
     1921 ccttccctgc cgtgcatcat ctggtcagca ccataacggc gcggctacca gaacagttac
     1981 acaccagcga tctgctgcgc gcagcttttc ctggtggctc aataactggg gctccgaaag
     2041 tacgggctat ggaaattatc gacgaactgg aaccgcaccg acgcaatgcc tggtgcggca
     2101 gcattggcta tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctaa
     2161 ctgccattaa cggacaaata tactgctctg cgggcggtgg aattgtcgcc gatagccagg
     2221 aagaagcgga atatcaggaa acttttgata aagttaataa gatcctacgt caactggaga
     2281 aataaacgtg gaacaccgta gcctgacgct tgatgatttt ttatcgcgct ttcaactttt
     2341 gcgtccgcaa atgaaccggg aaacgttaaa tcatcgtcag gctgccgtgt taatccccat
     2401 cgtccgtcga ccgcaaccgg ggttgttgct gactcagcgt tcgattcatc tgcgtaaaca
     2461 cgctggacaa gtggcattcc ctggaggcgc agtcgatgac acggacgcat cagttatcgc
     2521 cgccgcgctg cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat
     2581 cggcgtgctg ccgcccgtcg atagcgtaac tggctaccag gtaaccccag tggtcggcat
     2641 tatcccgccc gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat
     2701 gccgctcgcc caggcattac atctgggtcg ttatcatcct ttagatatct accgccgtgg
     2761 cgattcacat cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc
     2821 aggcataatt cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt
     2881 acatctacaa aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga
     2941 ttagtttaat tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct
     3001 gttattagtt cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct
     3061 attcgacatg tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa
     3121 ggcgggtaaa cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg
     3181 cgttgccgtg gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat
     3241 cgccattatt atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc
     3301 cggttttatt cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga
     3361 agtggatttc ccgcgcgaca acgggatgcg ttttcataac ggtaaccttc cgctgcatga
     3421 aaacggtatg caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta
     3481 ttccatcggc ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga
     3541 agtgagcgtg ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac
     3601 cggctattcg ctttctggtc tcgctatgca aaacgaactg gcgctgcaca gcaagaaaga
     3661 gatcgacgag tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat
     3721 gaataccgaa ggcgtactgc caggcccgtt gcgcgtgcca cgtcgtgcgt cggccctgcg
     3781 ccggatgctg gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt
     3841 aaacatgttt gcgctggcag ttaacgaaga aaacgccgcc ggtggccgtg tggtaactgc
     3901 gccaactaac ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat
     3961 tgaatcagtt agcccagaca tctatacccg ttactttatg gcagcgggcg cgattggtgc
     4021 actgtataaa atgaacgcgt ctatttccgg tgcggaagtc ggttgccagg gcgaagtagg
     4081 tgttgcctgt tcaatggctg ctgcgggcct tgccgagctg ttgggcggta gcccggaaca
     4141 ggtttgtgtg gcagcggaaa ttggcatgga gcacaacctc ggtctgacct gcgaccccgt
     4201 tgcagggcaa gttcaggtgc cgtgcattga acgtaatgcc attgcctctg tgaaggcgat
     4261 taacgccgca cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt
     4321 catcgaaacg atgtacgaaa ccggtaagga catgaacgct aaataccgcg aaacctcacg
     4381 cggtggtctg gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg
     4441 gatgggcgaa tttattcccg ctttctcgtc tgctgtaatt ttccccacta cacttccact
     4501 gttgcgtcag gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac
     4561 ggatcattaa aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta
     4621 cgctcgcttc gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg
     4681 tagtacaatt cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg
     4741 caggtagcga agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat
     4801 tacgtaaaca ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca
     4861 cactttattg ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg
     4921 aacttcctgc accgcaacca cttttacgcc tgacgaccga ccatgccctg attaaaggca
     4981 gtccggtttt gattcaatgg acgccagcag cgggcagtag caaagctggg gtcatggaga
     5041 tgattaacat cgacttactg gcggcaatgc tgcttgagcc acaactgccg caaatcagta
     5101 gcgccagcct gacggtggac gatcggcatt tgctctatgg taatgggctg gtagattccc
     5161 ttccgcaacc tgaaaacaat gaaaactatc aggtttcttc gcaacgcttt ccttttacca
     5221 ttaacgttaa tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac
     5281 cgctggcggt gctgctaagt ttacttgtgg gctatatcgc ctggctggcg accgcttacc
     5341 ggatgagttt ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct
     5401 gccagccttt gcttaatgcg cgcagccagc aatgtattgg cgtagagatt ttgctgcgct
     5461 ggaacaatcc gcgtcagggc tggatttcgc cggatgtgtt tattcctatc gcggaagaac
     5521 atcatttaat tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg
     5581 ttttcccgat gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc
     5641 gtggtgtgct gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac
     5701 tgatcctcga aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg
     5761 agctacatcg taaaaacgtc aaactggcga ttgatgactt cggcaccggc aatagttcat
     5821 tttcctggct tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag
     5881 ctataggttc tgacgcggtt aactcgacgg tgaccgatat catcatcgca ctggggcaaa
     5941 gactgaatat tgaactggtg gcggagggtg tggaaacgca agaacaggcg aaatatttgc
     6001 tccgtcatgg cgtccatatt ttgcaagggt atttgtacgc acagccgatg ccactacgtg
     6061 attttcccaa atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca
     6121 cacccattat gccgttacgt taacgcttac tcatcttcat cgtgcgccgg ttgctcttta
     6181 acaatgcgaa ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc
     6241 cctacatcaa tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc
     6301 gcgaccgtcg cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag
     6361 gcatgcaaat ctgtaccgcc ttttaccagc cagccgtcac cgtcggtaat gatttccggc
     6421 gtttcgtcag cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc
     6481 aagccttgta ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcacgacgc
     6541 agcacgccca gcaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc
     6601 gaagcaatcg ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt
     6661 acgattccga tgatttcatc cagttcaccg cgacataccg ggaacaggct gtgcggtgag
     6721 gagagcagct gctcgcggat ttcatctacc ccgagattag cgtcaaccca gcttatttca
     6781 ccgcgcggcg tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc
     6841 atgtaacgtt cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg
     6901 gcatcgtgct gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca
     6961 gtacgcgctc gcagcggcaa agtcgactgg tgacgaataa agttgcgacg cgcaatctgg
     7021 ttaaacactt cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg
     7081 tggaaaccga aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag
     7141 agcaccacca ccgtggggtg ctggttaaca aatcgcgtca gcggtttcga tgccagcagc
     7201 ataactgcca tcgcaatcac taccgccgcc atcatcaccg gcaaatggtt aaccatccct
     7261 actgcagtaa ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg
     7321 accacccaga aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc
     7381 cgttcatgca gttcggttgt tgctttgaac agcaagaata tgcccccgaa caacataatc
     7441 aggtcgcgtc cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc
     7501 atccatgaaa tcagcgacag cagccccaga cgcataatca gcgccagtga taatcccagc
     7561 aaacgcgctt tatcgcgttg ttttggcggc agtttgtcag caagaatggc aatgaagacc
     7621 aggttatcga tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt
     7681 gaggggtcca ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca
     7741 gaaataatgc aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gtgccagaaa
     7801 ggcaggcgtt aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta
     7861 ctgccctata ctccatggtt gttaaacggg agttaaacat atcagagacg cctctgattt
     7921 ggcaaagatt taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt
     7981 tctgctaatc gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct
     8041 aaaataaatc gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt
     8101 gtggacgacg attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt
     8161 agcaagtgac cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta
     8221 aaacggcaga aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag
     8281 gtgaaaatgc cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca
     8341 ctaaaggcgt gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc
     8401 gcattgtcgt cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg
     8461 tggaaacgtt aatggcccgt gatgatgacc caagctttga tgaactggtg gcgctggcag
     8521 tagaaacagg tcgtgaaggc gtgaaagcac tgaaagccaa accggttgaa aaagccgcgc
     8581 cagcacccgc tgccgcagca ccaaaagcga ctccaactcc ggcaaaacca atgggaccaa
     8641 acgactacat ggttattggc cttgcgcgta tcgacgaccg tctgatacac ggtcaggtcg
     8701 ccacccgctg gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg
     8761 ctgcggatac cgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac
     8821 acgtagttga tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac
     8881 gcgtaatgct gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga
     8941 aaatcacctc tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc caggtgaata
     9001 acgcggtttc ggttgatgaa aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta
     9061 ttgagctgga agtccgtaag gtttccaccg atccgaaact gaaaatgatg gatctgatca
     9121 gcaaaatcga taagtaacgt attgtgttga ttatcactca gttttcacac ttaagtctta
     9181 cgtaaacagg agaagtacaa tggagattac cactcttcaa attgtgctgg tatttatcgt
     9241 agcctgtatc gcaggtatgg gatcaatcct cgatgaattt cagtttcacc gtccgctaat
     9301 cgcgtgtacc ctggtgggta tcgttcttgg cgatatgaaa accggtatta ttatcggtgg
     9361 tacgctggaa atgatcgcgc tgggctggat gaacatcggt gctgcagttg cgcctgacgc
     9421 cgctctggct tccatcattt ctactattct ggttatcgca ggtcatcaga gcattggtgc
     9481 aggtatcgca ctggcaatcc ctctggccgc tgcgggccag gtactgacca tcatcgttcg
     9541 tactattacc gttgccttcc agcacgctgc ggataaggct gctgataacg gcaacctgac
     9601 agcgatttcc tggatccacg tttcttctct gttcctgcaa gcaatgcgtg tggctattcc
     9661 ggccgtcatc gttgcgctgt ctgttggtac cagcgaagtc cagaacatgc tgaatgcgat
     9721 tccggaagtg gtgaccaatg gtctgaatat cgccggtggc atgatcgtgg tggttggtta
     9781 tgcgatggtt atcaacatga tgcgtgctgg ctacctgatg ccgttcttct acctcggctt
     9841 cgtaaccgca gcattcacca actttaacct ggttgctctg ggtgtgattg gtactgttat
     9901 ggcagtgctc tacatccaac ttagcccgaa atacaaccgc gtagccggtg cgcctgctca
     9961 ggcagctggt aacaacgatc tcgataacga actggactaa caggtgagcg aaatggttga
    10021 tacaactcaa actaccaccg agaaaaaact cactcaaagt gatattcgtg gcgtcttcct
    10081 gcgttctaac ctcttccagg gttcatggaa cttcgaacgt atgcaggcac tgggtttctg
    10141 cttctctatg gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca
    10201 agctattcgc cgtcacctgg agttctttaa cacccagccg ttcgtggctg cgccgattct
    10261 cggcgtaacc ctggcgctgg aagaacagcg tgctaatggc gcagagatcg atgacggtgc
    10321 tatcaacggt atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg acccgatctt
    10381 ctggggaacc gtacgtccgg tatttgcagc actgggggcc ggtatcgcaa tgagcggcag
    10441 cctgttaggt ccgctgctgt tcttcatctt gtttaacctg gtgcgtctgg caacccgtta
    10501 ctacggcgta gcgtatggtt actccaaagg tatcgatatc gttaaagata tgggtggtgg
    10561 cttcctgcaa aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt
    10621 agttaacaag tggacgcatg tcaacatccc gctggttgtc tctcgcatta ctgaccagac
    10681 gggcaaagaa cacgttacca ctgtccagac tattcttgac cagttaatgc caggcctggt
    10741 accactgctg ctgacctttg cttgtatgtg gctgttgcgc aaaaaagtta acccgctgtg
    10801 gatcatcgtt ggcttcttcg tcatcggtat cgctggttac gcttgcggcc tgctgggact
    10861 gtaagactgt tgtacactac cggggccttt tggccccgtt tttttatctg gaggattaat
    10921 gacaatcacg gacctggtac tgattctttt catcgccgca ctcctggcct tcgcgatcta
    10981 cgatcagttc atcatgcccc gccgtaacgg ccccaccctg ctggcaattc ctttgctccg
    11041 gcgtggtcgc atcgatagcg ttatcttcgt cggattgatt gtcattctta tctataacaa
    11101 cgtcacgaat catggcgcgc taataacaac atggttatta agtgcactgg ctctgatggg
    11161 tttttatata ttctggatcc gcgttccgaa gatcatcttt aaacaaaaag gttttttctt
    11221 cgccaatgtc tggattgaat atagccgaat caaagcgatg aacttgtcgg aagatggcgt
    11281 gttggtgatg caattagaac agcgccggct gttaatccgc gttcgaaata tcgacgatct
    11341 ggaaaaaatt tataagcttc tcgtttcaac tcaataagtt atgaatttag ccaaagctat
    11401 gtttagtgta tttttaataa tcagacatag cttaggctat attacctctt cccttatttg
    11461 ttatttattt taacgtttca ttgatatata aatctaaat
//
LOCUS       DAZGTJ010000125        11486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000125 DAZGTJ010000000
VERSION     DAZGTJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.125"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(125..604)
                     /locus_tag="ACHK8E_004495"
     CDS             complement(125..604)
                     /locus_tag="ACHK8E_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346670.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(716..1960)
                     /locus_tag="ACHK8E_004496"
     CDS             complement(716..1960)
                     /locus_tag="ACHK8E_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP0346671.1"
                     /translation="MTLPVDSLIAYTLEKMQERIEQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEDRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQQILSSDSELSEKSDGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            complement(1978..2445)
                     /locus_tag="ACHK8E_004497"
     CDS             complement(1978..2445)
                     /locus_tag="ACHK8E_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346672.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGFLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(2449..2862)
                     /locus_tag="ACHK8E_004498"
     CDS             complement(2449..2862)
                     /locus_tag="ACHK8E_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFP0346673.1"
                     /translation="MAEINFQVLREAEQLTGVATAGRPVARHFDDGLTLEVNRTCTTG
                     ERNANSALYGAVWRAAKAMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAESS
                     VALKSTRDPVGSGGVPRVLREIRRMSTTVIHIKGE"
     gene            complement(2877..3485)
                     /locus_tag="ACHK8E_004499"
     CDS             complement(2877..3485)
                     /locus_tag="ACHK8E_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP0346674.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(3482..4207)
                     /locus_tag="ACHK8E_004500"
     CDS             complement(3482..4207)
                     /locus_tag="ACHK8E_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFP0346675.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGDRY
                     RRCVTFYGFSERPQVAAYAFDVLCRHLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(4200..5933)
                     /locus_tag="ACHK8E_004501"
     CDS             complement(4200..5933)
                     /locus_tag="ACHK8E_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP0346676.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTERKQHGITRPLKAVSDADTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(5933..7300)
                     /gene="dnaB-PI"
                     /locus_tag="ACHK8E_004502"
     CDS             complement(5933..7300)
                     /gene="dnaB-PI"
                     /locus_tag="ACHK8E_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFP0346677.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(7648..8550)
                     /locus_tag="ACHK8E_004503"
     CDS             complement(7648..8550)
                     /locus_tag="ACHK8E_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346678.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMFSASQAALFSQQTRQCASEQCTGRIQASCHHSGSHPDVSVYAERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            complement(8786..8995)
                     /locus_tag="ACHK8E_004504"
     CDS             complement(8786..8995)
                     /locus_tag="ACHK8E_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346679.1"
                     /translation="MCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDAFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(9328..10215)
                     /locus_tag="ACHK8E_004505"
     CDS             complement(9328..10215)
                     /locus_tag="ACHK8E_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0346680.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQIYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     REFFDALLENGGKSLWAEN"
     gene            10531..>11486
                     /locus_tag="ACHK8E_004506"
     CDS             10531..>11486
                     /locus_tag="ACHK8E_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0346681.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVL"
BASE COUNT         2709 a         3448 c         2685 g         2644 t
ORIGIN      
        1 cagcactgtt cactttttaa cctcttctgc cactggcagc actgttcact ttttgacctc
       61 tgctgccact ggcagcactg ctcatttttt aacctctgtt gccactggca acaccgctca
      121 ttttttaatc actgttgcca ctggcaacat ggttaatgat gactactacc ttaacgttca
      181 gagacaaaaa cagcattaat gtcgttatcc ccatcacgag caactgaatg tcggttatat
      241 ccagatgaga caatgaccag aaaatcacaa aaaatgaaaa gcattctgcc agcagactca
      301 ggaggagcat aaattgcagg ccccctccga ctgtactgcc tgtacgcgct ttataaagac
      361 tgtatgtgac ccacagccag ttacttaaca ccaggaaaaa ccaccctgtg gcacatacga
      421 tataacacat actgttatcg tactgttcgt gcattgtttt cagcgcagtg agcagggtct
      481 ctgtgcctgt caccagcccc cagataacca ccactccggc aatcacgcag gcacaaattt
      541 tagcgatcaa caggccactt tctttcatgg ctgcactcag actttcctgt ttgtatttct
      601 gcatgctatt gaccaccttg tttcacctga ccagttcccg attctgtttt ctttttaacc
      661 agtcgctggc ggcactggtt gatctttgtt cccgcgaaca tagcccgtat tgcaatcact
      721 cagatctcat cgcagaacat ctgcgcagcg aggcccgtag tccagccaca atgccactca
      781 gacgttctgc tgaaatcggt tccgtacgtt tccccgatga aactgattgc gcagtagctc
      841 gccctctttc tcctgccagt acggaggctg gcacaacaac acgttgtttc tgcttcggcg
      901 gataaaaatt tccctcccgg gctctcttca gcactgcaag caaccagccc acaggattct
      961 gcagcgagtg gctgcccagc atccctgaca gactttccag aaccccctgc gccagttctt
     1021 ccggcagcgc ctgcaactga ctggtcagca taatggcctc ctcctccctg atttgccctg
     1081 tcagcgcttc aggcaatacg tacgtgtttt taatcacact gtgtgtgata ttacgtacgt
     1141 aatggttcgg tttccgaact ctgtgataac catctgattt ttctgatagt tcggaatccg
     1201 aactcagtat ttgttgcccc tgtttttcac tgagttcgga atccgaactc agcattttca
     1261 ataccgaact ctgtgtttct acccccggtt cggtttccga acccggctgc attctgtcct
     1321 cagtcactcg ctgacgggcc gccatctgtc ccggtgtctg ggcactccct atccgggctt
     1381 ccagttgact gatatggctg tgataatgac gcattcccgg atcatttttt atttcgcaca
     1441 gaatatcccg ggccgtctga cttatggtcc ggttccggct catacaggca tctgccacca
     1501 tatccagata acgtggatcc agtatttcag catcccggca cgtcaccggt tcatcgtgct
     1561 gggcataaat attgccccgt acccgccctt tctcatcacg cacccgtttg cacagactca
     1621 gccagcccgt gatccgcagc atcagcagca cccggctgac agtctccctg gaggcttttc
     1681 ctttccccgg ggaagccagc tgcagttgca gttcgtcata tgtggggaaa actgcacctt
     1741 cattattctg tgcatacagc cgaatcatca tccacgccat cttatccagc ggagaaagtc
     1801 gagtatccag aagcagtcga cgcggaatgg catcatgcac atttccggta aacagcagtc
     1861 cgctttgtat ttttccgtcc tgttgtcggc ctttttgctc tatccgctct tgcatttttt
     1921 ccagcgtgta cgcaatcagg ctgtctacgg gtaatgtcat tacacttttc ctttttgtta
     1981 tacgtcagac atccgtcggc tggtcacggg tatatcgccc atattcacca gacagttacg
     2041 ccggagatat gccagcgctt catcaggaac ggtacattct ggtctggctt ttttcaggtt
     2101 actgattaat cgcttcatca gcattactgg ttcatgtgca cggatccacc gtccccctgt
     2161 ttgatcttcc agacagcgaa ttaacatctc agctggcaaa agaaacccgt ggttctcaca
     2221 ctcctttttc ttctctgccg cagttaattt ctgttgcaat tcaacaattg ctgccagcgc
     2281 agatttaatc gtttcactcg tcacccgaca tggataagca cgattcctgc cttctttcag
     2341 aagcactgca taaccatcag agcggtagtg ataacagttt gctcccaacc agatttcgta
     2401 agtatcgccg cgcgaggaga tacccgttct ctctgcaata gccatatctc attccccttt
     2461 gatgtgaatg acagtagtgc tcattctcct gatttcccgg agcacacgag gaacaccacc
     2521 gcttccaacc ggatcgcgtg tactcttcag ggcgacactt gattccgccc agctttttcc
     2581 tgaaggaagt tctttcacac gaacaaaacc agctgcccga agagaggctc cggattcatc
     2641 agccctggtg tatgtaatgc aacggcgata tcccatagct tttgccgccc tccagacagc
     2701 gccataaagt gcgctgttag cgttacgttc accagtagta caggtacgat tgacctccag
     2761 tgtcagccca tcgtcaaagt ggcgtgcaac aggtctgccg gctgtcgcca cacctgtcaa
     2821 ttgttcagcc tcccgcagta cctgaaagtt aatttcggcc atgtcatttg tccttgtcat
     2881 tctgcttttt cccggttcag tttccggcga ctggccgata catcattaaa ccccgcagtt
     2941 ccctgatgcc atcccctcac ggcatgccat accgccgtca gcgggatatt catttgctca
     3001 gctgccagca tcatgacatc cagtccgtcc gcactctcca catccgtgat gccgctcttc
     3061 tgccataaac gccacagctc tgcactttcc tcatcactca gtgtgatgcc acgtcccggg
     3121 cgaacatcaa taccggcaat gcgacggcgc gccgccacat ccacactgga aagtccaaag
     3181 taaaacgcca tcagttcaat ggagcctccc agcgccagtg cccggtcaat gcgctgcaga
     3241 cgtttctgct ccgtccgggc ctgctgcagc atacgcagca ggttttcatg attaatgtcc
     3301 agccggataa ccgacacttc agagtttgac aggtaatgaa tttcctcaat ggtcagtccc
     3361 tgcagcatct gcatttcctc ccgggccagc cccagagatt cgcagcgacg caggtagccg
     3421 ctcttgaggt ccatcaccag ttgcatcagc agaccgttgg ttgcctgaga caagctgtta
     3481 ttcatgacgc ctcccgtatc atcagacccg gcaccacctg tcgggcactc tcccccacac
     3541 catgatgcag tgaggcattt ttcccggcct catacccctg ccagcgggcc aggtcaccac
     3601 cgcgacaggc tttcatctca cgggagtcca ccgtcaccat atcctgctgc tccatccaga
     3661 gacgcatcag ccggctttcc tcttctgtca cactgaatgg catcaccacc tgacgaatcc
     3721 cctgaaccca gccatcacga aactgagccg cccgggcacg acgggtgctc agtttcagtc
     3781 tccggctctg tgtacgaaga tacgcatccg ttgcttccct tagatgacgg cacagaacat
     3841 caaacgcata agccgccacc tgaggacgtt cactgaaacc gtaaaacgtc acacaccggc
     3901 gataccggtc tccgggcacc gcccgccagt cgtaatacgc ccggcagcca aatgtctgtg
     3961 tcaccaccat gaccagacga aacatccacg ccggaacttt ttcagcatca gaaggtgccc
     4021 cctgtgatga agaggtctgc accgaagaaa gtccggcatc ctgctccgtg ataccgtgtt
     4081 tcgccatcag ctgttgcgcc cttgcaagcg ccagcccggc ctcatgggca ttgctgttat
     4141 tacggctcag ctccagcagt ttcctgaccc gcctgatcag acgttcctga tgcgacatat
     4201 cactcatcat tctcatcccc ctcatcgtcg tcgggtaaca cactgcgctg cagggctcgc
     4261 agacggcgca gcacactcag aagacgcagc agcttcacgg catggtcgtc atccagcagt
     4321 ggccacgctt ccggtgcact gccccctgta agcaggtctt tcagactcac ggggaggact
     4381 gtccgcggtg catcaccggc cagccccgcc agaaacgcca ctacaggaga acatttctcc
     4441 actgctgccc cgaaaccggc agacaggtcg ctttccctgt ccatggcaat ctcttcagca
     4501 caaccggctg attcagccag ttcccaggcc agacggaacg cttcattctg cagatgttcg
     4561 atatcatccc gccccacagc aaagtgcgtc accgacgcgg gaagagcatc agtggttccc
     4621 ggggcagtat ctcctgtcat aactgatgcc agtactgctc cggcagcacc atcttccata
     4681 acatcaccac tgaagactgg cttaccaccg tacatatccg gctgtatctc aggacgcagg
     4741 gcattttcag cagaagacgc cccactgtcc cccctgcctg cgctctcttc ctgagaaggt
     4801 cgactgcatt tttcagtgcg ctctgtgtca gcatcagaaa cggctttcag tggccgggta
     4861 ataccatgtt gcttgcgctc agtaccgggg aatatggaga ctgtgctttc cggcatcgcc
     4921 tctgtatccg tatccggttg acactccggt acttcagacc ggatcccgga taccggtgcc
     4981 ccgggttgta ccgtactgct gccacggact gaaggaggca ccaccaccgg ttccggctca
     5041 ccaaaatgat gacgacggtt tcgctctttc ggatccagtt ccagcaacca ccggtcatag
     5101 tccagggagg gatgtggcag ggcctgcaac aaatctccga taagctcatc gcggaacatc
     5161 tccagtgacc acagttccgg cgaattaaac ttccggcagc tttccccgaa cacgcggtca
     5221 aattccgcat ccgtatcggc tgcaacagca aactgccccc atacacgttc tgcgtcctga
     5281 cgaagtgcga ggagtgctgt aacctgtgga cgccccagtc ctgactccat caggtcgggg
     5341 atccaggggt acagatactt cagggcatct tccattctgc tgatcgtgga gttatgtatc
     5401 ggcaaccctt catgggttaa caaatcagcc agttcacgaa gagacactgt tctgcccagt
     5461 tgttcctcat ggatttgtct tgccttgtgg accccctgcg ccttctcaat aaaagtcagt
     5521 tcaccgcgaa cttcattttc agccagatga ccaatcacac actggagccg acccggccag
     5581 ggtttaaaca gcacatgaat acggtaaaaa cgcggatccc ctgtttcttt ccagagttcc
     5641 gtcagtatct ggtaacgggt gtttcccccg tcactgaaga tatacacatc cggctcactg
     5701 tccggcatac gggtgacttt gggcaccgta tccagcccgc gggcatgaat ggaatttttg
     5761 atatcgtcat aacgcggatt gcgcgtggtg cgcgggttat ccggattcgg acgtaactgg
     5821 tccagcgtca gaaccatggg cgtttccccc attgccacca cattgccggc accagtggtc
     5881 tgacgaccgg gctgcataat agccgccccg agatccagtg gtttatgtgc catcacgcat
     5941 atcctcccgg cccgtcattc tcagagggcg taaacagtgt gtaacgaccg tcgaacctga
     6001 cccgtaccgt acccgtgggc ccctgacgct gcttgctgac aatgatttct gcctccccct
     6061 ttgccagtga gtcggggtta tagacctcgt cgcggtaaat aaacataatc aggtcagcat
     6121 cctgctccag cgccccggaa tcacgcaggt caccgttatt ggggcgttta tcagcacgct
     6181 gctccacctg ccggttaagc tgggagaggg ccagcaccgg gcagcccagc tccttcccca
     6241 gcgctttcag ggaacgggaa atttcagcaa tttcctgtgt gcggttttcc agctccggcg
     6301 cacgaatcag ttgcagataa tccaccataa tcagggaggg tttaccgtac agacgtgtat
     6361 aacgacgggc gcctgcacgc agtgatgaag gcgtctgctc acagcagtca tcaattatta
     6421 tgcgctcacc cagagagccc attcctccgt caccaagcgc cataccgacc gcagatgaca
     6481 ccctcagcca gtcctcatca tccatgttgc cactgcgcaa atgattcagt tccacacgcc
     6541 ccagcatgga aagcatgcgc agaatcagtt gctccttcgg catctcaatg ctgaatacca
     6601 gaacgctctc ttccgttcgc cgcagtgcgg cgctgagaca catggataac gccagagagg
     6661 ttttccccat tgacggacgg gcagccagca gaaccaggtc accaggctgc aggccacagg
     6721 tcatttcatc cagacagtca aatcccgtgg gggtaccggt aacaccaccg gagccgagca
     6781 cactttccag ttgctggaga accgtatcaa gcgcggatga aagagaggct tccgtgcgct
     6841 cccggagcgt cctgttctgc gcaagcgcaa acagacgccg ctctgcagca tccagcacat
     6901 cccgtgacgt ggcatttggt gccgccacat cagcaatcag ggtgtttccg gtggcctgta
     6961 actgacgcag aacgcttttc tccaccacaa tgcctgcata ggccatgatg ttggccgcag
     7021 aaggtgtgtt cttgctgagc tcggccagat aagcaaagcc gcccagtacg tccagcctgc
     7081 cctgatgttc caggtgctct gacagtgtaa tcaggtccag tggctggcca ttcaccgcaa
     7141 gttcagacat ggcacggaaa atggcccggt gctgagccac ataaaaatcg tcaggactca
     7201 gcatcagcac cacttcatcc cagcgttcat tctccagcat cagaccgccc agaacggcct
     7261 gctctgcttc gtatgcgcag agaacagaca tttcagccat ggcgtaccct ccctctctga
     7321 ctgccggaaa cctggctacg tgaaaaaagg aaaaagggtg ccggacgtac agctgtcggg
     7381 aataaaatcc cggtaactgt ctgtacgttt tcagaccagt aagtctggca accccggtct
     7441 gaaagtacag acagcattcg ttcattcata acaagttgtt ttaaaggaaa aagtttatga
     7501 atacagaaaa acaactgccg tccggcattg aatctgaggt gaaatacacc ctgcagttct
     7561 ggctgctgag taacggaaag gtcatgacgg aaaaagacgg gctctgtaac ccctttcccc
     7621 tgaactgtat tgaatcggct catcctttta ctccgtttca gcagaatcag tgggttgaac
     7681 tggacactgt cgggccgaat ggaccggtga aagcgacatt ccttgtgcac agcgttctgc
     7741 ataaactgaa acgtcagggt ggctccctga atgatggcaa ctggcctgaa tacggccggt
     7801 gcattgctca ctggcacact gacgtgtttg ttgagaaaaa agcgctgcct gactggctga
     7861 gaacattcca cactgacgtt tcgcactcgt aaaaacacgt tcagcccgac gggtcatatt
     7921 ttccagccac ccgtcgggga aaatgtcagc accatcacgg ggaaacaaaa tcgctttcca
     7981 ttcagggtat gagcgcggaa tacctccgga agcattccgg ctcagacagc gactcacagt
     8041 gcaggcagca cggaatgcat atgttgcgat gctctcatca tatgttgcga tgttctcatt
     8101 attgcctttc tgccgttcca cccgctcttc agcatcaaat gccagtgcag caaggcttgc
     8161 ccagcacatg cagttcactg aattccccgg aacgctctgc agctcccggc gcaccatgtt
     8221 gacctcagca ctgaaaaaag cgttctcatc gccttcaagg gcagccagat gcagcggaag
     8281 ccagacctga ctgaaccatt cacacatccg ttcccggtta ctgacggtca tcactgtctc
     8341 cgggcgggaa ccgtcctgaa aaacatgtcc ggtgcgtgga gaactgtatg aaacataacc
     8401 accgtgcacc gcaatgaccg gacgacctga cgccggaagc cccacacgac acccactggc
     8461 aatccgggtc atcagcaggt tcagttcagc cagcgattca gggcgggtta agaccgccgg
     8521 aaccggagaa tatttcactg aggaagacat actttcacct ccatatctct cagcccgcca
     8581 ggtgcgtcac ttccccgcag ataaccggaa acctggctat atgaaaaaag ggaaaagggt
     8641 gccggacgaa cagctgtcgg gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg
     8701 taagtttggc gaccctggtc agaaggtacg gacagcatct ttgcgctcat aactgtaccg
     8761 accgggatat gcattacaaa gactgtcata ccgccccctc cgtctttcgc tgtttgcagg
     8821 aaagaccaca tgaaaaaagc tggtcatgcc gctgacgacg ctggcgttcc tgcaaaagaa
     8881 aggcgtcaca accggaacac agccccagac cactctcagg attcaccttc acccagaccg
     8941 gagcattctt accggcacca tcccgtaccc cggaataacc acagcccacg cacaccgtcc
     9001 acttctgcag tacgatgagt gcagtttcga gtgatgcgcg gagacgccgg gaggcaataa
     9061 ggagcaacgg ctcatggtca taaaaaaccc cgccgttacc ccctccggat tcaagcagcg
     9121 ggagtcctgc aggctcccgg ggacctgttg acatttcccg taattcgttt tcattccaca
     9181 tattgtggaa agttatccgg ttcactgaac aacgccattc ctccgccaga cgctgataca
     9241 aatgcttttt cgcttctgcg cggtcagatg catcaacaaa cacatgcact tcctcgtgtc
     9301 cgggtgctga agccacggca cgccatatca gttttctgcc cacaggcttt tccctccgtt
     9361 ttccagcaga gcatcaaaaa actcacgcca ctccggcaac agttcacagg ccagactgtg
     9421 catggtgacc agcgcagaat cgctgcaacg ggcagacact ttttccagac gatgcaccgg
     9481 ctgaccggct gcgtgcccct gtttataaat attcaggtcg taaatctgtg ttgaaagaag
     9541 ggtgaccgca ccttcaggca gcactttgct gtagcgaccg gcgttgataa tgcctgtcag
     9601 ctctgccagt gtctgtctgg ccagcgccgt ataatccatg cagttaacca gggtgcggac
     9661 tttcggcagg ggaataccca gttcacggta gggcataagg ttttccatga cattgaccgt
     9721 gccgcggatg aactcacgca catccggcag aaccggattc accatcccca ccacagactc
     9781 cgtggcggac agcacaatca tttccagcat gacagaacgt gcgccctgtg aatccaccac
     9841 aatgacgtca tagatctgaa aaagcggatg ctgaagcaca ttacgcagac gaaggcgacc
     9901 atccggcgca tgaagcatgg ccgttttcag ttgctcatgg gggtcgttgg agacgatgag
     9961 gtcaagcccc ctgacagccg tacgggaaat gatgttgtca ggatgactga ggtcagccgt
    10021 gcgcatcagt aattcaaaaa gaccggccgg cgcttcatac gtcagcggaa agatattgct
    10081 tgccgtgggc tgtgcatggt ctccgtctat cagcagagta cggaggccgg catctgccag
    10141 aaaaccggca agattagcag actgggttga ttttccttcg ccacctttag tggatataac
    10201 cggtaaaatt ttcatatagc acactcttta aagtatgcaa ccaatcactt acactacgga
    10261 aaccagccag aatattcact tcactacctg aatactcgca acacggggat tgaaaatccc
    10321 cgtgtccttg gttcgattcc gagtccgggc accactttca cttccagatt catctcccga
    10381 actccacgaa actcaagtat aatcacatcg ttacaagcaa tctgaacatc ctgaactcca
    10441 ccgaactcta ctgaactctt tctcaatcaa gctttcattg ggggcaccgg tgggggctca
    10501 ttatgttcag tcagtttcag gagcccccat atggcactta cagccagaca ggtcgaaaca
    10561 gccagaccta aagaaaaaga ctataaactc tctgacgaac gtggtttata tctgctggta
    10621 aaaaccacgg gagcccgcta ctggcggctt aaataccgga tagcaggaaa agagaaaaaa
    10681 ctggccctcg gcgtctatcc cgacgtctcc cttgctgagg ccagaatcaa acgtgacgat
    10741 gcccgaaaaa tcatctccga aggtggtgac ccgggcgaaa agaagcgaaa ggaaaaactc
    10801 actcagaaaa tctctgccac caatacgttc catgccctcg ctacggaatg gcaccagcat
    10861 aaatctttgt catggtctga aagttacgcc agaagcgtac tggaagcgct ggataaagat
    10921 attttcccgt atctgggcaa acgaagcgtt acggatatcc tcccgctgga aatgctggaa
    10981 attctgcgcc gcatagaaaa acgtggctcg ctggaaaaac tgcgtaaggt acgtcaatac
    11041 tgtaatcaga tttttcgtta tgccatcgcc accggacgag ccactgtcaa tccggcatct
    11101 gaactgacca gtacgctggc ggcgccaaaa gctgcacact tcccccacct gagagcagat
    11161 gagctccctg tttttctcag gaagctcgct gaatatcatg gcagtcctgt tacccgcatg
    11221 gcgacaaatc tgctgcttct gacaggcctc agaacgattg agctacggtc cgctgaatgg
    11281 tcagaaattg attttgataa tgccctgtgg acaatccctg aaagccgcat gaaaatgcga
    11341 cgtaaacatg tcgtaccact gtcacgacag gtcactgaca ttctgcaaca actcaaaact
    11401 ttctccggac aataccggct ggttttcccg ggacgttgtg atatcaacaa gccaatgagc
    11461 gaagccagca tcaatatggt gctcaa
//
LOCUS       DAZGTJ010000126        11198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000126 DAZGTJ010000000
VERSION     DAZGTJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.126"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(62..343)
                     /locus_tag="ACHK8E_004507"
     CDS             complement(62..343)
                     /locus_tag="ACHK8E_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP0346682.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHK8E_004508"
     CDS             complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHK8E_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0346683.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1847..3880
                     /gene="metG"
                     /locus_tag="ACHK8E_004509"
     CDS             1847..3880
                     /gene="metG"
                     /locus_tag="ACHK8E_004509"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0346684.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4022..7810
                     /locus_tag="ACHK8E_004510"
     CDS             4022..7810
                     /locus_tag="ACHK8E_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFP0346685.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7820..>11198
                     /locus_tag="ACHK8E_004511"
     CDS             7820..>11198
                     /locus_tag="ACHK8E_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFP0346686.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSAT"
BASE COUNT         2908 a         2894 c         2743 g         2653 t
ORIGIN      
        1 cttaaatcat tcatgtgaat gatttaagct ttctaaatcc gattaaaacc agggtggcaa
       61 attaatcgta gcgatttaac acgattcgac gtttgctatc gctatctgca gcaatcactt
      121 ccgtcgttac acctttaact ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta
      181 actgaccaca atgctgttga ggttgaccat taaccatagc catactgcat gtcgggggac
      241 tgatcctacc attaatcgtt aatgtggcag tttctgaaga gaacgcggga gaggccaatc
      301 cataggcaag aaaaataatt cctgacagcc aatacttatt cattgaacgt tatccctgta
      361 gtaaaggtta tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca
      421 cagccacgaa ggatgataat gaagcattgc ctgtacgatc aatcgacttt gtagagtttc
      481 ggacgaaggt ccgcagaata ttcgcagcat taaataagtg ttcattaatg cggcaaaaac
      541 aaatacaccg catcaatgta acatctctgc ggtgtatgaa gaataaccag aatggttaat
      601 acgcgttaga ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtaaagc
      661 tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctcaggacgg
      721 ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc
      781 tgccccagca gctgtgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa
      841 atcggttcat ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg
      901 agcaccggaa cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg
      961 atgtcctgcg gggtggtgac cacgactgca ccggttactg gaatgttttg tgccaatgtc
     1021 aattgaatgt caccggtacc tggcggcata tcaagcacca gatagtcgag atccggccac
     1081 aaggtttcct gcaacatttg cattaacgcc ttgctcgcca ttgggccacg ccacaccatc
     1141 gcattgtcgt ctgtcaccag ataaccaatt gaattagttg ccaggccatg agacataatc
     1201 ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat tttctgcacc cagcatgtta
     1261 gggattgatg ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc
     1321 agtgccaacg ccaggtttac cgccgtggag gatttaccca cgccaccttt accggaactg
     1381 accgcgataa tgttcttcac gccgttaatt cccggctgat tcttcacgcg tttcagagtg
     1441 gctatgttat gcgacagttt ccagtcgata gctttcgctc cggtgatgcg cagcagttcg
     1501 gcgctacatt gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt
     1561 tccacatgca gcgtgtcgtc catccaggcg acatggtgca acgctttcag cgttgtgagg
     1621 ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag
     1681 gcttctggtg atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa
     1741 accatcagtt gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat
     1801 ttggtactat ctaagccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc
     1861 gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca
     1921 tatgctggag cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga
     1981 ggtcaacttc atctgcgccg acgatgccca cggtacacca atcatgttga aagctcagca
     2041 gcttggtatc actccggagc agatgattgg cgaaatgagt caggagcatc agactgattt
     2101 cgcaggcttt aacatcagct atgacaacta tcactcgacg cacagcgaag agaaccgcca
     2161 gttgtcagaa cttatctact ctcgcctgaa agaaaacggt tttattaaaa accgcaccat
     2221 ctctcagctg tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggcac
     2281 ctgcccgaaa tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac
     2341 ctacagcccg actgaactga tcgagccgaa atcggtggtt tctggcgcta ccccggtaat
     2401 gcgtgattct gaacacttct tctttgatct gccctctttc agcgaaatgc tgcaggcatg
     2461 gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc
     2521 tggcctgcaa cagtgggata tctcccgcga cgccccttat ttcggttttg aaattccgaa
     2581 cgcgccgggc aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt
     2641 caagaatctg tgtgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga
     2701 ctccactgcc gagctgtacc acttcatcgg taaagatatt gtttacttcc acagcctgtt
     2761 ctggcctgcc atgttggaag gtagcaactt ccgcaagccg accaacctgt ttgttcacgg
     2821 ctatgtaacg gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag
     2881 cacctggctg aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc
     2941 ttcgcgcatt gatgatatcg atctcaacct cgaggatttc gttcagcgtg tgaatgctga
     3001 tatcgtcaac aaagtggtta atctggcgtc ccgtaacgcg ggctttatca acaaacgttt
     3061 tgacggcgtg ctggcaagcg aactggctga cccgcaactg tacaaaacct tcactgatgc
     3121 cgctgaagag attggcgaag cgtgggaaag ccgtgaattt ggtaaagcag tgcgcgaaat
     3181 catggcactg gctgacctgg ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc
     3241 gaagcaggaa ggtcgcgatg ctgacctgca ggcgatttgc tctatgggca ttaacctgtt
     3301 ccgcgtactg atgacttacc tgaagccggt actgccgaaa ctgactgaac gagcagaagc
     3361 attcctgaat acggaactga cctgggatgg tatccagcaa ccgctgctgg gccataaagt
     3421 gaatccgttc aaagcactgt ataaccgtat cgacatgaag caggttgaag cactggtgga
     3481 agcgtctaaa gaagaagtga aagccactac cgcgccagtt actggcccac tggcagatga
     3541 tccgattcag gaaaccatca cctttgacga cttcgctaaa gttgacctgc gcgtggcgct
     3601 gattgaaaac gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cgctggatct
     3661 cggcggtgaa aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaggc
     3721 acttattggt cgtcacacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg
     3781 tatctccgaa ggcatggtga tggctgctgg tcctggcggg aaagatatct tcctgctaag
     3841 cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa tccccccttc aaggcgctgc
     3901 atcgacagcg ccttttcttt ataaattcct aaatttgttt tcttgcgatt ttgtctctct
     3961 ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag
     4021 aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcgg tggagcagca
     4081 gggaaacgaa ctgcacatca actggggtaa agtcggcacc aacggacaaa gccaggtaaa
     4141 aagttttgcg gatgcagcag cggcggaaaa agcgggactg aagctgattg cagagaaggt
     4201 gaagaagggg tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac
     4261 ggactctctc aaggtagctg acttatccac cattattcag gaacaaccct ctttcgtagc
     4321 agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat
     4381 tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg
     4441 agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc
     4501 gcaacgggat aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa
     4561 caccgtcgcc caggcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc
     4621 cgtaatggct gtactcactg cacttgaaat gaaatgcaca agatataaag agcgtgagga
     4681 tgttatggat cagattattc aggaaggcgg tctggaatat gctactgatg taataataca
     4741 tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt tcctgtcgtc
     4801 tggcatttca cctgattact tgcagcaata ttccagcttt gaattacgcc tacgtaaaca
     4861 tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta ttgccgcaat
     4921 tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg aaaaaccaga
     4981 aattgcacat gaaatttccc agcgtctact ggggcaaaag aaattaccct cgcttgagtg
     5041 gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc
     5101 atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga
     5161 gcaaggtgtt acagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga
     5221 tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca
     5281 tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac acgcagcact
     5341 ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca ttatgctaat
     5401 gacgatgctt atctcgcatc cgacactagc agaacaggtc attccctggc tttcgacacc
     5461 cgcagttgca gtgctgaaat catgtcagca acaactgaca cagccctcaa accatgccag
     5521 cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa agaaaaaatc
     5581 gccgattccg gtgctggatt tagcaccgct caacctcgaa tccatatgca caataaccga
     5641 cacagaagct aaagagtttc aaactcattg ggactgggaa ccgcataagc caagtgaagg
     5701 tgctaaaaat ttcctttact cgctggggta tcgccgctgg gattttgaca catataaata
     5761 tatcggcgct tctgactcgg caattgacgc atgggaacgt gaggacttcg ctacactcat
     5821 ccaaatgttt aaagcccacc atgcaccgta tcagggggag tggcatctca actcacttcc
     5881 ctttttacct atgcaaaaag cgattaagct atgggaattt ctcagcaaag agccgcatac
     5941 cgcgataaaa ccagtcatgc tttatttgcg actggcaggt atgagtggtt ttctacactc
     6001 gttttcacgc taccctcaag aaggttttgc ggttgctaat tattttgccg caacggaact
     6061 ggctcctgcc gtcgcccgcg ccttcaacaa actcaaaaca ttacgacaag acgccagtag
     6121 ctggctgctg aaatacccgg aacacgccat taccggcctg ctgcctgcag cgctcggcaa
     6181 agccagtgaa gcacaggaca acgcccgtgc tgccttgcgt atgcttaccg aaaacggtca
     6241 tcagccattg ctgcaagaaa tcgcccggcg ttataaccag ccggaagtaa ccgatgcggt
     6301 gaacgctcta cttgcgctcg atgccttaga taatcacccg acaaaaatcc ccactcttcc
     6361 gaccttttat cagccatcgc tctggacgcg cccgttatta aaagcaaacg cccaatcact
     6421 gcctgatagc gcactcctcc acctcggtga aatgctccgc ttccctcagg aagaggcact
     6481 gtatccggga ttattgcagg tgaaagatgc ctgtaccacc gactcactgg ctgaatttgc
     6541 ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt
     6601 cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc ctttgattcg
     6661 cgcctggcct ggtgaatccc agcataaacg cgccaccgtc gggttggata ttctcgctgc
     6721 tatcggtagt gatatcgccc tgatgcagct taacggcatc gcccagaaac tgaaattcaa
     6781 agcattacag gagcgggcaa aagaaaaaat tgccgacatt gccgagagcc gcgaactcac
     6841 ggtggcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata atggttcgtt
     6901 gttgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt
     6961 tgtgcgtgac gcttccggca gtcgtctgaa agacctgccc aaaccaaaca aaagcgatga
     7021 tgaattacag gcgaacaatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac
     7081 cgtcgccgcc cagcaggtag caaggctgga atccgccatg tgcctgcgtc gccgctggtc
     7141 accggaaaac tttcagctct tcctggttga gcatccgctg gtccgccact taacccgccg
     7201 tctgatttgg ggcgtttata gcgccgaaaa ccagctactg gcttgctttc gcgtagcgga
     7261 agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc
     7321 tattggtatt cctcacgttc tggaaatatc accgacggat gctgccgcct tcgggcagct
     7381 ttttgccgac tacgaactgt taccaccgtt ccgccagctt gatcgtaaca gttacgcgct
     7441 gacagaagcc gagcgcaatg ccagcgaact gacacgctgg gcaggcagaa aatgcccgag
     7501 tggtcgggta atggggctgg caaataaagg ctggatgcgt ggtgaaccgc aggacggtgg
     7561 ctggatcggc tggatgatca aacctctggg ccgctggtcg ttaatcatgg aaatcgatga
     7621 aggttttgcg gtcggcatgt cgccagccga actcagtgcc gagcaactct taagcaagct
     7681 gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa cacaggaagc
     7741 gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata ttgaagccct
     7801 gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata acgcccaact
     7861 ggaactgaaa tataaaaaag gcaaaacacc actcagccat cgcaattggc caggcgaacc
     7921 ggtacctgtt atcactgaaa acatcattca gacactgggt gatgaattgc tacaaaaagc
     7981 agagaagaaa aaaaacattg tctggcgtta tgaaaacttt tcactggagt ggcagtccgc
     8041 catcacgcag gccatcaact tgatcggcga acacaaaccc tcaatcacgg cccggacaat
     8101 ggcggcgcta gcctgtatcg cgcaaaatga tagccaacag ttgctcgacg aaatcgtcca
     8161 acacgagggg ctggaatatg caactgaggt ggtgattgca cgccagttta ttgtacggtg
     8221 ttatgagagt gatcctctgg tagtaacatt gcaatatcag aacgaggact atggctatgg
     8281 ttatcgctca gaaacctata acgaattcga tctccgattg cgtaagcatc tctctctggc
     8341 agaggaaagc tgctggcagc gttgcgccga caaactcatt gccgcactgc caggaataac
     8401 caaagttcgc cgccctttta ttgcgctgaa cctcccggaa aaacccgaaa tcgccaacga
     8461 gctggtaagc cttgaatgtt cgcaaactca ttttcgctca aaagagtggt taaaagttgt
     8521 tgctgatgac cctaaggcgg tgaaagaact cgcacgttac tggagccagg atatatttag
     8581 cgatcgagaa gccagctaca tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc
     8641 ccttttgcgc gaacaaggac tggcagccgt tccgcgcctc gcgatgtatg cacataaaga
     8701 agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca ccttgctact
     8761 ggtggctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta aaaacttccc
     8821 ccatgcgacg ctcgccgcac tggcagaact gctggcgtta aaagcccccc ctgcgcgccc
     8881 tggttatccc atcatcgaag accaaaagct gcctgcacag caaaaagcac gtgatgaata
     8941 ctggcatacg ctgttacaaa cgctgatggc atcgcagcca caactggcag aagaggtgat
     9001 gccgtggtta agtactcaag cccgggcagt gctgaatagt tatttatcgg caccgcccaa
     9061 aacggttatt gatagcaccg ataacagcca aatgcctgaa atcctcgttt caccaccgtg
     9121 gcgtagtaag aaaaaaatga cagctccacg tcttgatttg gcaccgctcg aattaacccc
     9181 gcaagtttac tggcaaccag gcgaacaaga gaggcttgcc tccactgagt ctgcccgtta
     9241 tttcagcacg gaatctcttg ctgaacgcat ggaacaaaaa agtggacgag ttgtattaca
     9301 ggaactgggt tttggggatg atgtatggct gtttctgaat tatatactcc ccggaaaact
     9361 ggatgctgca cgcaattcac tcattgttca gtggcattac tacccagggc gggttgaaga
     9421 gatcatgaat ggctggagct ccccggaagc tcaattagca gaacaggcgc ttcgcagcgg
     9481 tcacgtagaa gtgttaatta acatatggga aaatgacagc tactcacgtt atcgtccgga
     9541 aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg ctttgccttt
     9601 ctggctacgt atcaatgaga aaaagcatct gtctgcgggt gaggactatt ttctcagtat
     9661 cttcggattg gatgctctac caggtctgct gttggctttt tcacatcgtc caaaagaaac
     9721 atttccgtta attttaaatt ttggcgcaac agaactggcc ctgcccgttg cccgcgtctg
     9781 gcgacgtttc gcggcgcagc gtgatctggc ccgccagtgg attttacaat ggccggaaca
     9841 tacggctact gcacttatcc ctcttgtctt caccaaaccc agcgataata gcgaagctgc
     9901 attacttgcc ctacgtttac tttacgaaca gggacatggt gaattgctgc aaaccgcggc
     9961 aaaccgctgg cagcgtacag atgtatggcc tgccctggag catttactta aacagagtcc
    10021 aatagagatt tacccggcac gcatgccaaa agcccctgat ttctggcatc cgctaatgtg
    10081 gtccaggccg cgcctaatca ttaatcatca acttattacc gatgacgctc tggaaattat
    10141 cggcgaaatg ctgcgcttta cccagggggg acgttttcat agcgggctgg aacaactgaa
    10201 aacgttctgc cagccgcaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca
    10261 acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga
    10321 cgaaagcacg gcacgggatc taacgacaca gatcctcgcc tggccacaag aaggcaaatc
    10381 tacccgtgct gtcagtggcc tgaacatcct taccctgatg aataatgata tggcgctgat
    10441 acagctgcat catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga
    10501 atttcttcag gtggtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt
    10561 agtcccaaca ctgggccttg atgatccgca ggcgttgagt tttgattttg gtccccggca
    10621 gtttaccgtt cgcttcgatg aaaaccttaa cccggttatc tttgatcagc aaaacgttcg
    10681 ccagaaaagc gttccccggt tgcgcaccga tgacgatcaa ctgaaagcgc ccgaggcact
    10741 ggcccgatta aaagggctaa aaaaagatac gactcaggtg agcaaaaacc tgctcccccg
    10801 tcttgaagct gccctgcgta ccacccgacg ctggtcgctg gcagattttc attctctgtt
    10861 tgttaatcat ccctttaccc gtctggttac ccagcgatta atatggggcg tgtatccggc
    10921 aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa
    10981 tgcgcaagat gagcccattg acctgcctgc ggatgctctg attggcattg cccacccgtt
    11041 agaaatgaca gaaggaatgc gcagtgaatt tgcacagctt tttgccgatt acgaaattat
    11101 gccgcctttt cgccagttgg cgcggcgcac ggtgctgctc acacctgacg agtcaaccag
    11161 taacagcctg actcgctggg aaggtaaatc cgctaccg
//
LOCUS       DAZGTJ010000127        11159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000127 DAZGTJ010000000
VERSION     DAZGTJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.127"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            183..287
                     /locus_tag="ACHK8E_004512"
     CDS             183..287
                     /locus_tag="ACHK8E_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346687.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            545..2743
                     /gene="speF"
                     /locus_tag="ACHK8E_004513"
     CDS             545..2743
                     /gene="speF"
                     /locus_tag="ACHK8E_004513"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP0346688.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2740..4059
                     /gene="potE"
                     /locus_tag="ACHK8E_004514"
     CDS             2740..4059
                     /gene="potE"
                     /locus_tag="ACHK8E_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP0346689.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4116..5756)
                     /gene="pgm"
                     /locus_tag="ACHK8E_004515"
     CDS             complement(4116..5756)
                     /gene="pgm"
                     /locus_tag="ACHK8E_004515"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP0346690.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5782..6327)
                     /gene="seqA"
                     /locus_tag="ACHK8E_004516"
     CDS             complement(5782..6327)
                     /gene="seqA"
                     /locus_tag="ACHK8E_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP0346691.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6512..7276
                     /gene="ybfF"
                     /locus_tag="ACHK8E_004517"
     CDS             6512..7276
                     /gene="ybfF"
                     /locus_tag="ACHK8E_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0346692.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7416..7709
                     /gene="ybfE"
                     /locus_tag="ACHK8E_004518"
     CDS             7416..7709
                     /gene="ybfE"
                     /locus_tag="ACHK8E_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP0346693.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7849..8379
                     /gene="fldA"
                     /locus_tag="ACHK8E_004519"
     CDS             7849..8379
                     /gene="fldA"
                     /locus_tag="ACHK8E_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP0346694.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8589..8675
                     /gene="uof"
                     /locus_tag="ACHK8E_004520"
     CDS             8589..8675
                     /gene="uof"
                     /locus_tag="ACHK8E_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP0346695.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            8668..9114
                     /gene="fur"
                     /locus_tag="ACHK8E_004521"
     CDS             8668..9114
                     /gene="fur"
                     /locus_tag="ACHK8E_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP0346696.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9198..9524)
                     /gene="chiQ"
                     /locus_tag="ACHK8E_004522"
     CDS             complement(9198..9524)
                     /gene="chiQ"
                     /locus_tag="ACHK8E_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP0346697.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9574..10980)
                     /gene="chiP"
                     /locus_tag="ACHK8E_004523"
     CDS             complement(9574..10980)
                     /gene="chiP"
                     /locus_tag="ACHK8E_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP0346698.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2731 a         2799 c         2738 g         2891 t
ORIGIN      
        1 atacggcttt ttatcttaaa caacacacaa aaataacaat tcaatatttt atattactga
       61 gtaaaaagct catcattaaa taaattaaga ttgatcattt tttattgatc accttcacag
      121 ttcaaccgta tttcctggat agaatacctt caccttcatt cacatcagga aaggtaaatt
      181 aaatggaaaa taacagccgc actatgcccc atataaggcg gacaactcat attatgaagt
      241 ttgctcatcg caatagcttc gactttcact tcttcaatgc ccgttagtct accgactaag
      301 ggcacttcag cgtacaggtc ttcctgactc tctgtattac aggcatatgc ctgtattccg
      361 cagtgctcat tctctgttaa cccttgtatt atcagccacc ggactgataa gggtttctgc
      421 attctgaaaa aagcaaactt ctgattaatt tatcagcggg gtaactttgc tttttttccg
      481 gcacgatcga tttctcattc gagaaattga ggacctgcta ttacccaaaa taaagagatg
      541 gaaaatgtca aaattaaaaa ttgcggttag tgattcttgc ccggactgtt ttaccacgca
      601 gcgagaatgt atctatatta atgaaaacag taatatcgat gtggcggcaa tagttttatc
      661 gctcaacgat gttacacgcg gaaaactcga tgaaatcgat gccacgggtt atggcatccc
      721 ggtatttatt gctactgaaa atcaagaacg tgtacccgca gagtatttgc cccgtatttc
      781 gggtgtcttt gagaattgcg aatcgcgacg agaattttat ggtcgtcagt tagaaaccgc
      841 agccagccat tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt
      901 caatcagggt aacagcgcgt ttgattgccc cggtcatcag ggcggcgaat ttttccgtcg
      961 ccatcccgcg gggaatcagt ttgtggaata ctttggtgag gcgctgttcc gtgctgactt
     1021 gtgcaacgcc gacgtagcga tgggcgatct gctgattcac gaaggcgcgc catgcattgc
     1081 acagcaacat gcggcaaaag tgtttaatgc cgataaaaca tacttcgttt taaatggcac
     1141 ttcatcttct aacaaagtgg ttttaaacgc cctgctgaca ccgggtgatc tggtgctgtt
     1201 tgaccgcaat aaccacaaat ctaaccacca cggagcgttg ctacaggctg gcgcgacacc
     1261 ggtttatctg gaaaccgcac gtaacccgta tggttttatc ggtggtattg acgctcactg
     1321 ttttgaagaa agttacctgc gtgaactgat cgcggaattc gcaccgcagc gggcaaaaga
     1381 ggctcgtcct ttccgcctcg ccgttattca gctaggcact tacgacggta cgatttataa
     1441 cgcccgtcag gtggtggata aaattggtca tctgtgtgac tacatcctct ttgactccgc
     1501 atgggtcggc tatgagcagt ttatcccgat gatggcggac tgttcaccac tgttgctgga
     1561 tcttaatgag aacgatccgg gtattctggt tacgcaatct gtgcataaac agcaggctgg
     1621 tttttcccag acttcacaaa tccataaaaa agacagccat atcaaagggc aacagcgtta
     1681 tgtaccgcac aaacgcatga acaacgcctt tatgatgcat gcctccacca gcccgttcta
     1741 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     1801 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactaatcc tcgataactg
     1861 tcagcatatt cgtccattcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
     1921 aacggcgcaa attgcggttg atctgcgctt cttccagttt gtaccaggtg agcactggca
     1981 ttcttttgaa ggctatgcag agaatcaata ctttgtcgat ccgtgcaaac tgttgctgac
     2041 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
     2101 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2161 cctctttctg ctgactccgg cagaagatat ggccaaactt cagcaacttg ttgctctgct
     2221 ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta tcagaagtac tgccttccat
     2281 ctacaaacag catgaagagc gctacaccgg ttataccctg cgtcagttgt gtcaggaaat
     2341 gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     2401 acacttccca cgcgtaagca tgaatccaca agaagccaac tacgcctatt tacgcggtga
     2461 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     2521 ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg ctgttctgcg
     2581 ttacttcagc gctctggaag aagggatcaa cctgctgcca ggttttgcac cagagctgca
     2641 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2701 tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaat
     2761 aagatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     2821 atcatgctgc cgacaaagct tgccgaagtt gggacaatct caattatctc ctggctggtg
     2881 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     2941 aaatcaggcg gtatgggcgg ttatgcggag tatgctttcg gtaaatccgg gaactttatg
     3001 gcgaactaca cctacggtgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     3061 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3121 accatcggag tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3181 caaatcagta gcattactgt gtggggggtc attattccgg tcgtcggtct gtgcattatc
     3241 ggctggttct ggtttagccc gacgctatac gctaactcat ggaacccgca ccatgtgccg
     3301 ttctttagcg ctgtaggttc ttccatcgcc atgacgttgt gggctttcct cggtctggag
     3361 tcagcctgtg ccaacgccga agtagttgaa aacccggaac gtaacgtgcc gattgcagtt
     3421 cttggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgccggg
     3481 attgtgccaa atatggagct ggcaaattca acggcaccat ttggtctggc cttcgcgcag
     3541 atgttcacgc cggaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3601 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttctgc cgatgaaggg
     3661 tacttcccga aaattttctc ccgtgtgagc aaagtcgatg caccagtaca gggaatgctg
     3721 accattgtca tcatccagag cggattatca ctgatgacca ttagcccgtc gctgaacagt
     3781 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     3841 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     3901 gcaaactttg ttgcttttgt tggcgcgatg tacagctttt atgcgctgta ctcatccggg
     3961 gaagaagcca tgctatacgg ttccatcgtg accttcctcg gttggacact gtatggtctg
     4021 gtctcaccac gctttgaact gaaaaataaa cacggttaat taagcaaagc aaaaaagggc
     4081 gatcttccga ccgccctttt tcttatttga tatgtttacg cgtttttcag aacttcgcta
     4141 acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccaag gaagctttcg
     4201 cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca
     4261 gtcatcactt tcagaccgcc gatagaagcg ccattacccg gtgccgccgt cagacgcgcg
     4321 gtgatcgggt cacctgccag ggtgctggcg ctcaccattt ccggagacag cttagacaac
     4381 gccgcttttt gtgcggaagt cgctgctgcc tgcaaacggt tatagctcgg cgcaccaaag
     4441 cgttttgcca gttcgttata gtgttcctgc gggttcttac cggtgacagc ggtgatttcc
     4501 gccgccagca gacacataat gataccgtct ttatcggtgg accacggcgt gccgtcgaaa
     4561 cgcaggaagg aagcgcctgc actctcttcg ccgccaaagc cgaagctgcc atcgaacaaa
     4621 ccatcaacaa accatttgaa acctaccggt acttctacca gcttacggcc caaatcattg
     4681 accacacggt cgatcatcgc agatgaaacc agcgttttac cgacagcaac atctttgccc
     4741 cactgcggac gatgctggaa caggtagttg atcgccaccg ccaggtagtg gttcggattc
     4801 atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc
     4861 agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc
     4921 atacggatcg caccgtcttt atcgaggtgc ataaagcgga aggtttgatc gacctgatcg
     4981 ttaacgatgg tcaggttgag gttgtaatac tcgccaatgc gcttccagta ttcgataccg
     5041 gaaccaccca gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc cgccatatca
     5101 acgatatcgg ccagcccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg
     5161 gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc agccagcagt
     5221 gcgttggctc tgtcttccac cactttagtg acgttggtat cagccgggcc accatttggc
     5281 ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat cacgataccg
     5341 tctgccagtg ggccaccttt tttattgtga accagaatgg cattggaaat ggcaggtgtc
     5401 ggagtgaagc cattgttttc ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc
     5461 agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat ctttacccac atagcaaggg
     5521 ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc
     5581 ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc
     5641 gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg
     5701 acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct
     5761 ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg
     5821 gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt
     5881 ggtgatcacc caatacggcg taccaggcac atgtttcggc ttggtctgat taccattttt
     5941 cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt
     6001 tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg
     6061 attgaccgct cgcttttgct ctgcgtattc gtccgaaagc agaagttcac gcattgcgcg
     6121 aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg
     6181 aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg
     6241 taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag
     6301 ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa
     6361 gtcgccagga gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa
     6421 aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct
     6481 aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc
     6541 acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gcagcctcga
     6601 caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat
     6661 gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga
     6721 tcttgttgat accctggatg cacagcagat cgacaaagca acatttatcg gtcactccat
     6781 gggcggtaaa gcggtaatgg ctcttactgc actagccccc gatcgcattg acaagctggt
     6841 ggcgattgat attgcgccgg tcgattatca cgttcgccgt catgatgaga ttttcgcggc
     6901 catcaacgca gtcagtgaat cagacgcaca aacacgccag caagcagcgg caataatgcg
     6961 ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga
     7021 gtggcgcttt aacgtgccag tattgtggga tcagtatccg catattgtgg gttgggagaa
     7081 aatcccggca tgggatcatc ctaccctgtt tatccctggc ggcaattctc cgtatgttag
     7141 cgagcagtac cgtgatgatt tactggcaca atttccacag gctcgggcgc atgtgattgc
     7201 aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg
     7261 ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt
     7321 ggacgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcaggcg
     7381 cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta
     7441 cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaact aatccgcttt
     7501 cgcgcgatga gcagctgcgt attaataaac gcaaccaact aaaacgcgac aaagtacgtg
     7561 gccttaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcactg aacgagctgg
     7621 cggaatcgcg caatatgagc cgtagcgagc tgatcgaaga gatgttaatg cagcaactgg
     7681 cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt
     7741 cctgctcgtt tgctgattcc gcaagactgc ctgttctgct atgattgcct ttatccgtgg
     7801 gcaattttcc acccccattt caataagttt caagaggtta tttcactcat ggctatcact
     7861 ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa
     7921 cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg
     7981 gaagcatatg acattctgct gctgggcatc ccaacctggt attacggcga agcgcagtgt
     8041 gactgggatg acttcttccc gactctcgaa gagattgatt tcaatggtaa actggttgcg
     8101 ctgtttggtt gtggcgacca ggaagattac gcagaatact tctgcgatgc attaggcacc
     8161 atccgcgaca tcattgaacc gcgcggcgca actatcgttg gtcactggcc aaccgcgggc
     8221 taccacttcg aagcgtcaaa aggtctggct gatgatgacc actttgtcgg tctggctatc
     8281 gacgaagatc gtcagccgga actgactgct gaacgtgtag aaaaatgggt taaacaaatt
     8341 tctgaagagc tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat
     8401 gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt
     8461 gctgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct
     8521 gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca
     8581 attctataat gatacgcatt atctcaagag caaattctgt cacttctcct aatgaagtga
     8641 accgcttagt aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct
     8701 ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag ttcttcagga gccggacaac
     8761 catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt
     8821 ctggctacgg tatatcgcgt actgaaccag tttgacgatg ctggtatcgt cacccgccac
     8881 aattttgaag gcggtaaatc cgtatttgaa ctgacacagc aacatcacca cgatcacctg
     8941 atctgcctcg actgcggcaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag
     9001 cgtgaaatcg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt
     9061 cactgtgccg aaggcgattg ccgcgaagat gagcacgcgc acgaaggcaa ataagccagc
     9121 ctgaacgaga aaagccaacc tgcaggttgg cttttttata caagggaaat aagaaaagcg
     9181 agagttacag ctctcactta tttgttatta ctgcgaattt cctgccagac cttatcacaa
     9241 tcggctttca ccgcctgatc atttccggtt tgcgttgcct gaatacactg ctgataatcc
     9301 agtacgcgga cactttcctg ctcggcaaac tgctgatgtt gtttctcttt cttcagcacg
     9361 tttaacacgc tctggcaggc ttcaattttt tccggcgaac cttgcgcggt gttgatacag
     9421 gcgctgtaag cctctttcag acgactgtct tcctgcggtg cggttgattg cgcacaagct
     9481 accagccccg atgccatcat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaat
     9541 gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
     9601 cataaatttc acgtcgcgct catcctggaa gatgttaccg taaccgccgc cccagcttgg
     9661 gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg
     9721 accgtcctga atggtgtaca ccgcatccag gctgtaggca gactcttcaa tagtccggtt
     9781 tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat cccatgcgta
     9841 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
     9901 accgaagaag accgcttttt cgccgttggc gttgaagtca gaacggttat cccaccagat
     9961 atccaggcga ccgttcgagg aagcgtaggt tggagtcata cgttgcagga agtatccctg
    10021 ctgaccgtcg gctttaaccc agctgccttc cagacgcaaa tccactacat cagccgcccg
    10081 gtaaccaaag gtcaacgctt gcagccaggc agtgccgtcg taaatgtcgt tgacgctgcg
    10141 atcgtcaact ttatcgcgcg taccgtagaa ctggtagctg gtggttaacg ggctaccggc
    10201 gatatcaaat ttgtagctgg ctttggcaaa atattgatct atataccctt ccgcctgacc
    10261 aaatgccgct tccagtacaa agttattttt gaagtcgtac ttcgcaccga tggagtgcag
    10321 ataatcaact ttggtagttt tatcgttctg ataaaactca tccatttcga gatgccacgg
    10381 tgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtaatc
    10441 aaaattcgcc ccggcttccg caccctgata agtacccggc ataaagctcc agtgcggcgc
    10501 taacagcgtc tgaccagttg gctgaatgta acctgccctc gcccacaccg gaccgtattt
    10561 aaatttggcc gctgctttat acaggcttat accgcttttg tcgccggacc agtcttcgtc
    10621 ataggcttta ttgctttttg aaaacgcgat ttcgttcggg tgagagctgt caccgttttc
    10681 cgccatttca atcgccgtaa acgcagcaat atcaaggccg aacatatcag cagcataacc
    10741 ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt tgtatttgtc
    10801 gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac cgccggttaa
    10861 ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa agcccgacat
    10921 tgctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa acgtacgcat
    10981 gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaaataaa
    11041 aattaaaaaa gtagcgttga gaaaagtctc gctgactaca ggaacagact atttttcgcg
    11101 acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa ccgcacata
//
LOCUS       DAZGTJ010000128        11140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000128 DAZGTJ010000000
VERSION     DAZGTJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.128"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            230..1372
                     /gene="yghO"
                     /locus_tag="ACHK8E_004524"
     CDS             230..1372
                     /gene="yghO"
                     /locus_tag="ACHK8E_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP0346699.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1379..2143)
                     /gene="glcC"
                     /locus_tag="ACHK8E_004525"
     CDS             complement(1379..2143)
                     /gene="glcC"
                     /locus_tag="ACHK8E_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP0346700.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2394..3893
                     /gene="glcD"
                     /locus_tag="ACHK8E_004526"
     CDS             2394..3893
                     /gene="glcD"
                     /locus_tag="ACHK8E_004526"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP0346701.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3893..4945
                     /gene="glcE"
                     /locus_tag="ACHK8E_004527"
     CDS             3893..4945
                     /gene="glcE"
                     /locus_tag="ACHK8E_004527"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP0346702.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFSPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4956..6179
                     /gene="glcF"
                     /locus_tag="ACHK8E_004528"
     CDS             4956..6179
                     /gene="glcF"
                     /locus_tag="ACHK8E_004528"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP0346703.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASADRTS
                     VRHWIEIVEQALEKE"
     gene            6184..6588
                     /locus_tag="ACHK8E_004529"
     CDS             6184..6588
                     /locus_tag="ACHK8E_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP0346704.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6610..8781
                     /gene="glcB"
                     /locus_tag="ACHK8E_004530"
     CDS             6610..8781
                     /gene="glcB"
                     /locus_tag="ACHK8E_004530"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP0346705.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            9137..10819
                     /gene="glcA"
                     /locus_tag="ACHK8E_004531"
     CDS             9137..10819
                     /gene="glcA"
                     /locus_tag="ACHK8E_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP0346706.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(10847..>11140)
                     /locus_tag="ACHK8E_004532"
     CDS             complement(10847..>11140)
                     /locus_tag="ACHK8E_004532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346707.1"
                     /translation="VFIKRFNKQKSNNYSQCNYPKLLIVLTIAIAKATDSTVFAHQAN
                     IISTNLFCVIKNRYSHVLNYLAGYLNAKHYCCMDNKEGNNFQLIRQNNVNVAG"
BASE COUNT         2602 a         2759 c         3102 g         2677 t
ORIGIN      
        1 tttattgtga atgatattta atttatgaat atattaagaa ggagccttgt ccggtcgcct
       61 taaatcgaca ggaggcattg tggcgcttct gtgtagtctc tgaccttatc gtaatcatta
      121 ataaatacta tttattcttt gtttgtattc attgggttga ttattaagat gccgttcgtt
      181 gctgttgcaa cctgtttttc accgttaatt atttgttatc cacagagata tggaatgcga
      241 tctgctgatg attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc
      301 ttttccgtca tcactttatc ccgatgatcc aaactggata ccccctttat tcattgaacg
      361 cagcgagcat ttgtctgcga agaatccggg gacggaccat atcatctggc aggcgtgggt
      421 ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg caaatcgata ccttgcaccg
      481 tgagcgttac ggtgaagata ccggtcatta cggaatgatt gatgccattg atgatccgca
      541 ggtttttgcc gcgttgtttg gcgcagcgga agcgtggtta aagtcacaag gtgcaaataa
      601 gatcagcggg cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt
      661 tgacactccg ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga
      721 acaactgggt tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct
      781 cacgttctct ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaaag tgaccattcg
      841 ctgcatcaat cgccagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaattc
      901 tggctggcag cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg
      961 cgatcaactt aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc
     1021 accctgcgcg tttattgttg gcttgccgaa catcaacgag gcgattgccg atctgaacgg
     1081 atcgctcttt cccttcggct gggcaaaatt gctgtggcgt ttgaaagtca gcggtgtgcg
     1141 aactgcgcga gtgccgctga tgggcgtgcg ggacgagtat cagttcagcc gcatcggccc
     1201 ggtaattgcg ctgttattga ttgaagcctt acgcgatccg tttgcccgtc ggaagatcga
     1261 tgcgctggag atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg
     1321 cattggggcg gagccgtaca agcgttaccg attgtatgaa aagcagattt gatggcagtc
     1381 acccctgctt catctccagc ggcaccgccg agcgaatcaa atggtggcct tccagctcga
     1441 tatcgtggag attctttttc acggtccgca catgatcacg tgctgcgcgc tgggcgacgt
     1501 gcggcagccg ctgcaacacc gcgttgtaga tccgcgcatg ctggcgatcg atctgctttt
     1561 tttgctgtgg tcgatggtag agattattta cgctggcaaa cactgaatta aacatcagat
     1621 cggtcaatga ttgcagcgta aacaccagca cctgattgtg agaagcctga caaatggcga
     1681 gatggaaagc gtgatccaac tgcgcatgtt cgatcagcga aatctctttg ttgttctcac
     1741 tggcggcgag cattttttca taacagcggg ttatcacaac aaaatcagcc tgggttccca
     1801 gcgttgccgc cagccttgcc gattcgccct ccagtaatgc gcgaacgtcg agcagatcgt
     1861 acagcgttcg cggctgcgta ctgaacagat ggatcagcgg gctggcgtcc tgcacccgat
     1921 taagccgtgc gacacgagaa tcgcgaccct gcgccgtttc aataatcccg cgcccgcgca
     1981 gcacggtcag cccttcacgc agtgcggagc gtgagaagcc gagcttttca cacagtcgac
     2041 gttccgaggg aagcggctga ccgaccttca acacgccgtc gataattaac cgttcgatac
     2101 tctctgcaac cacttcgcaa atagggcgac gttcatcttt cattcccgga cctcgtgcac
     2161 aggtaggacc aatttttctg ttcttaactc gcaaaacacg cacatcacgc aagtgattat
     2221 gttacatcaa tttaacatta agttaaccaa ggcaaggtca cagagctgga aaaaaaatgg
     2281 tctgaccggt agctaaagag atagacgaaa acgaaaagcc cgcttaataa ctgttcacag
     2341 aagcagcgcg caaaaatcag ctgccacaca acacaacaaa gcgaagccta ttcatgagca
     2401 tcttgtacga agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga
     2461 tggcactgcg tgagcatgtc cctggacttg agatcctgca taccgatgag gagatcattc
     2521 cttacgagtg tgacgggttg agcgcgtatc gcacgcgtcc attactggtt gttttgccaa
     2581 agcagatgga acaggtgacg gcgattctgg ctgtctgcca ccgcctgcgt gtaccggtgg
     2641 tgacccgtgg tgcaggcacc gggctttctg gtggcgcgct gccactggaa aaaggtgtgt
     2701 tgttggtgat ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt cgccgcgcgc
     2761 gcgtgcagcc gggcgtgcgt aatctggcga tctcccaggc cgttgcgccg cataatctct
     2821 attacgcgcc ggatccttcc tcacaaatcg cctgttcaat tggcggcaat gtggcggaaa
     2881 atgccggtgg cgttcactgc ctgaaatatg gtctgaccgt acataacctg ctgaaaattg
     2941 aagtgcaaac gctggacggc gaggcgctga cgctgggatc ggatgcgctg gattcgcctg
     3001 gttttgacct gctggcgctg ttcaccggat cggaaggtat gctcggcgtg accaccgaag
     3061 tgacggtaaa actgctgccg aagccgcccg tggcgcgggt tctgttagcc agctttgact
     3121 cggtagaaaa agccggactt gcggttggtg acatcatcgc caatggcatt atccccggcg
     3181 ggctggagat gatggataac ctgtcgatcc gcgcagcgga agattttatt catgccggtt
     3241 atcccgtcga cgccgaagcg attttgttat gcgagctgga cggcgtggag tctgacgtac
     3301 aggaagactg cgagcgggtt aacgacatct tgttgaaagc gggcgcgact gacgtccgtc
     3361 tggcacagga cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat gcgttcccgg
     3421 cggtaggacg tatctccccg gattactact gcatggatgg caccatcccg cgccgcgccc
     3481 tgcctggcgt actggaaggc attgcccgtt tatcgcagca atatgattta cgcgttgcca
     3541 acgtctttca tgccggagac ggcaacatgc acccgttaat ccttttcgat gccaacgaac
     3601 ccggtgaatt tgcccgcgcg gaagagctgg gcgggaagat cctcgaactc tgcgttgaag
     3661 ttggcggcag catcagtggg gaacatggca tcgggcgcga aaaaatcaat caaatgtgcg
     3721 cccagttcaa cagcgatgaa atcacgacct tccatgcggt caaggcggcg tttgatcccg
     3781 atggtttgct gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg
     3841 ccatgcatgt gcatcacggt catttacctt tccctgaact ggagcgtttc tgatgctacg
     3901 cgagtgtgat tacagtcagg cgctgctgga gcaggtgaat caggcgatta gcgataaaac
     3961 gccgctggtg attcagggaa gcaatagcaa agccttttta ggccgccctg tcaccgggca
     4021 aacgctggat gtgcgttgtc atcgaggcat tgttaattac gacccgaccg agctggtgat
     4081 aaccgcgcgt gccggaacgc cgctggtagc cattgaagcg gcgctggaga gcgccgggca
     4141 aatgctcccc tgcgagccgc cgcattatgg tgaagaagcc acctggggcg ggatggtcgc
     4201 ctgcgggctg gcggggccgc gtcgcccgtg gagcggttcg gtccgcgatt ttgtcctcgg
     4261 cacgcgcatc attaccggca ctggaaaaca tctgcgtttt ggtggcgaag tgatgaaaaa
     4321 cgttgccgga tacgatctct cacggttaat ggccggaagc tacggttgtc tgggtgtgct
     4381 cactgaaatc tcgatgaaag tgttaccgcg accacgcgcc accctgagtc tgcgtcggga
     4441 aatcagcctg caagaagcca tgaatgaaat cgcccagtgg caacttcagc cattacccat
     4501 tagtggctta tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc
     4561 ggtaaaagca gcgcgtgaac tgctgggggg cgaagaggtt gcgggtcagt tctggcagca
     4621 attgcgtgaa caacaactgc cgttcttctc gttaccaggt accttatggc gcatttcatt
     4681 acccagcgat gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggggc
     4741 gttacgctgg ctgaaatcga cagccgatga caatcaaatc catcgcatcg cccgcaacgc
     4801 tggcggtcat gcgacccgct ttagtgccgg agatggtggc ttttccccgc taccggctcc
     4861 tttattccgc tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaaccc
     4921 cggtcgcatg tacgcggaac tttgaggagc aggctatgca aacccgatta acagaagaga
     4981 tgcggcagaa cgcgcgcgcg ctggaagccg acagcatcct gcgcgcctgt gttcactgcg
     5041 gattttgtac cgcaacctgc ccaacctatc agcttctggg cgatgaactg gacgggccgc
     5101 gcgggcgcat ctatctgatt aaacaggtac tggaaggcaa cgaagtcacg cttaaaacac
     5161 aggagcatct cgatcgctgt ctcacttgcc gcaattgtga aaccacctgt ccttctggtg
     5221 tgcgctatca caatttgctg gatatcgggc gtgatattgt cgagcagaaa gtgaaacgcc
     5281 cactgccgga gcgaatgctg cgcgaaggat tgcgccaggt agtgccgcgt ccggcggtct
     5341 tccgtgcgct gacgcaggta gggctggtgc tgcgaccgtt tttaccggaa caggtcagag
     5401 caaaactgcc tgctgaaacg gtgaaagcta aaccgcgtcc gccgctgcgc cataagcgtc
     5461 gggttttaat gctggaaggc tgcgcccagc ctacgctttc gcccaacacc aacgcggcaa
     5521 ctgcgcgagt gctggatcgt ctggggatca gcgtgacgcc agctaacgaa gcaggctgtt
     5581 gtggcgcggt ggactatcat cttaatgcgc aagagaaagg gctggcgcgg gcgcgcaata
     5641 atattgatgc ctggtggccc gcgattgaag caggtgtcga ggcaattttg caaaccgcca
     5701 gcggttgcgg cgcgttcgtt aaagagtatg ggcagatgct gaaaaacgat gcgttgtatg
     5761 ccgataaagc gcgtctggtc agtgaactgg cggtcgattt agtcgaactt ctgcgcgagg
     5821 aaccgctgga aaaactggca attcgcggcg ataaaaaact ggccttccac tgcccgtgta
     5881 ccctacaaca tgcgcaaaag ctgaacggcg aagtggaaaa agtgttactt cgtcttggat
     5941 ttaccttaac ggacgttccc gacagccatc tgtgctgcgg ttcagcggga acatatgcgt
     6001 taacgcatcc cgatctggca cgccagctgc gggataacaa aatgaatgcg ctggaaagcg
     6061 gcaaaccgga aatgatcgtc accgccaaca ttggttgcca gacgcatctg gcgagcgccg
     6121 atcgtacctc tgtgcgtcac tggattgaaa ttgtagaaca agcccttgaa aaggaataac
     6181 aaaatgaaaa ctaaagtcat tcttagccag caaatggcga gtgcaattat tgccgcaggt
     6241 caggaagagg cgcagaaaaa taactggtct gtttccattg ctgttgccga tgacggcggt
     6301 catctgctgg cgttaagtcg catggacgat tgcgcgccga ttgcggctta catctcccag
     6361 gagaaagcgc gtaccgccgc gctggggcgt cgtgaaacta agggctatga agagatggtg
     6421 aacaacggac gtaccgcgtt cgtgactgcg ccgttactga cgtcgctgga aggtggcgta
     6481 ccggttgttg tggatgggca aattattggt gccgtgggcg tttctggttt aaccggagca
     6541 caggatgcgc aggtcgcgaa agcggcagca gcggtgttgg cgaaataagc gaaaacgagg
     6601 agataaacaa tgagtcaaac cataacccag ggccgtttac gcattaacgc caattttaaa
     6661 cgttttgtgg atgaagaagt tttaccggga acagggttgg acactgcggc gttctggcgc
     6721 aattttgatg agatcgttca cgatctggca ccagaaaatc gtcagttgct ggcagaacgc
     6781 gatcgcattc aggcggcgct tgatgagtgg catcgcagca atccggggcc ggtaaaagat
     6841 aaagcggcct ataaatcttt cctgcgtgaa ctgggctacc tggtgccgca accggagcgc
     6901 gtgacggtgg aaaccacggg cattgacagc gaaatcacca gccaggcggg gccacagctg
     6961 gtggttccgg caatgaacgc ccgctacgcg ctgaacgcgg cgaacgctcg ctggggctca
     7021 ctgtacgatg cgttatacgg cagcgacatc atcccgcagg aaggggcgat ggtcagcggc
     7081 tacgatccgc aacgcggtga gcaggttatc gcctgggttc ggcgtttcct cgatgaatca
     7141 ctaccgctgg aaaacggcag ctatcaggat gtggtggcgt ttaaggtggt cgataaacaa
     7201 ttacgcatcc agttgaaaaa tggtaaagaa accacgttac gtaccccggc gcagtttgtc
     7261 ggttaccgtg gcgatgccgc tgcgccgtcc tgcattttgc tgaaaaataa cggcctgcat
     7321 attgagctac aaatcgatgc caacgggcgg attggcaaag acgattcggc gcacatcaac
     7381 gatgttatcg tcgaagcggc catcagtacc attctcgact gcgaagattc ggttgcggcg
     7441 gttgatgcgg aagataaaat cctgctgtac cgcaatctgc tgggtctgat gcaggggact
     7501 ctgcaagaga aaatggagaa aaacggtcgg caaatcgtgc gtaaactgaa tgacgatcgt
     7561 cagtacaccg ctgccgacgg ctctgaaatt tctctgcacg ggcgttcgct gctgtttatc
     7621 cgcaacgtgg gtcatttgat gaccattcct gtgatttggg acagcgaagg caatgaaatc
     7681 ccggaaggca ttcttgatgg cgtcatgact ggcgcgattg ccctctatga tttaaaagtg
     7741 cagaaaaact cgcgcactgg cagcgtctat attgtgaaac cgaaaatgca cggtccgcat
     7801 gaagtggcgt tcgccaacaa actgtttacc cgcattgaga caatgctcgg tatggcaccg
     7861 aataccctga aaatgggcat tatggatgaa gaacgtcgga cctcgctgaa cttgcgtagc
     7921 tgtatcgctc aggcgcgtaa ccgcgtggcg ttcatcaata ccggtttcct cgaccgtacc
     7981 ggcgatgaaa tgcattcggt gatggaagct ggcccgatgc tgcgtaaaaa ccagatgaaa
     8041 tcgacgccgt ggatcaaagc ctacgaacgt aataacgtgc tttccggtct gttctgtggg
     8101 ctgcgcggta aagcgcaaat cggtaaaggc atgtgggcaa tgccggacct gatggcagac
     8161 atgtacagcc agaagggcga ccagctgcgt gccggggcaa acacggcctg ggttccgtcg
     8221 ccaactgccg ctacgctcca tgcgctgcac taccaccaaa ccaacgtaca gagcgtgcaa
     8281 gccaacattg cccagaccga gtttaatgct gaatttgaac cgctgctgga cgatctgctg
     8341 actattccgg ttgctgaaaa cgctaattgg tcggcgcaag agatccaaca agagctggat
     8401 aacaacgtgc aggggattct ggggtacgtg gtgcgttggg tggagcaggg gattggttgt
     8461 tcaaaagtgc cggatattca caacgtggcg ttgatggaag accgcgcaac gctgcgtatt
     8521 tccagccagc atatcgccaa ctggttacgt cacggtattc tgaccaaaga tcaggtgcag
     8581 gcgtcgctgg agaatatggc gaaagtggtt gatcagcaaa acgctggcga tccggcttat
     8641 cgtccaatgg tggagaattt cgctaactcg tgtgctttta aagctgcctg cgatttgatc
     8701 ttcctcggag tgaaacagcc aaacggctat accgaaccgt tattacacgc atggcgttta
     8761 cgcgaaaaag aaaatcatta atagcgtcaa aaaaagcccc ggcaagattt attgtcgggg
     8821 ctatgatagt aactgcggta gtacatttat cccggaaata accggagcga ttatcagggg
     8881 ttaacaatat taaatacccg tagcgcatca ctagcgggat taaaacaaca atatggacat
     8941 cttaacgata ctctgcgcct gcgtgtgcgg aacagaaacc cttacccgat atacttgtta
     9001 tgccagaggt agcaattgcg ttagcggcgt actgttgctg taaagtataa cgcctatatt
     9061 ttacagttca gtcctctttt gcttattaca accttaagta tatcaagcat ataaagataa
     9121 taagagactg aacaatatgg ttacctggac ccaaatgtat atgccgatgg gaggactggg
     9181 gctatccgct ctggtcgccc tgatcccaat aatattcttc ttcgttgcac tcgcggtatt
     9241 acgtctgaaa ggacatgtcg ctggagcaat aacccttata ttatctattc tgattgcgat
     9301 attcgccttt aaaatgccga ttgatatggc atttgctgct gcgggctatg gctttattta
     9361 tggtttatgg cctattgcgt ggattattgt cgcggcggta ttcctgtata aattaaccgt
     9421 tgccagcggc cagttcgata ttatccgcag ctcggttatc tccatcaccg acgatcagcg
     9481 tttgcaggtg ttactgattg gtttctcctt tggtgcgctg ctggaagggg cggctggctt
     9541 tggcgcaccg gtggcgatta ccggtgctct actggtgggc ctgggcttca aaccgctata
     9601 cgcggcgggg ctgtgtctga ttgccaacac tgcgccggtg gcgtttggtg cgttgggtgt
     9661 gccaattctg gtcgccggtc aggtaacggg aatcgatccg ttcgacattg gcgcaatggc
     9721 gggacgtcag ttaccgttcc tgtcggttct tgtgccgttc tggctggtag caatgatgga
     9781 cggctggaaa ggggtgaaag agacgtggcc agcggcgctg gttgcagggg gaagcttcgc
     9841 tgtcactcag ttctttacct ctaactatat tggtccggaa ctgccggata ttacttcggc
     9901 gctggtgagt atcgtctcac tttctttatt tcttaaagtc tggcggccaa aaaataccga
     9961 aacggcaatc agtatgggac aatccgcagg tgcgatggtg gtgaataaac cgtcttctgg
    10021 cggtcccgtg ccttcagaat atcgtctggg gcaaatcatt cgtgcgtggt caccgttttt
    10081 aatcttaacg gtgttggtca ccatctggac catgaagccg tttaaagcgt tatttgctcc
    10141 gggcggcgcg ttttattcgc tggtgattaa tttccagatc cctcatttgc atcaacaagt
    10201 gttgaaagcg gcccccattg tcgcccagcc gacgccaatg gacgcggtgt ttaaattcga
    10261 ccccctctct gctggtggca ccgctatttt tattgcggcg attatctcga tcttcatcct
    10321 cggcgtgggg atcaagaaag gtattggtgt ctttgccgaa acgctaatta gtctgaagtg
    10381 gccgatactg tcgattggca tggtgctggc atttgccttc gtgaccaact attccggcat
    10441 gtccaccacg ctggcgctgg tactggctgg tacaggcgta atgttcccgt ttttctcacc
    10501 gtttctcggc tggctgggcg tattccttac cggctcggac acctcctcta acgccctgtt
    10561 tggttcactg caatcgacca cggcgcagca aatcaacgtc tctgacaccc tgctggtggc
    10621 agcaaatacc agcggcggcg taactggcaa gatgatctcc ccgcaatcta tcgccgtggc
    10681 ctgcgccgcg acgggcatgg tgggccgaga atctgaactg ttccgctaca ccgtgaagca
    10741 cagtctgatt tttgccagcg ttatcggcat tatcacgctg ttgcaggcgt atgtgtttac
    10801 cgggatgtta gtttcgtaag tcaataatgc cggatgatcg ttaatatcat ccggctacat
    10861 ttacattatt ctgtcttatt aattgaaaat tatttccctc tttattatcc atgcagcaat
    10921 aatgttttgc gttcagataa cctgctaaat aattaagaac gtgagaatat ctatttttta
    10981 tcacgcaaaa caagtttgta ctaattatgt tcgcttgatg agcaaacacc gtgctatctg
    11041 ttgcttttgc aatcgcaatc gttaaaacta tcaacaactt gggatagtta cattgtgaat
    11101 aattgttact tttttgtttg ttgaatcttt ttataaatac
//
LOCUS       DAZGTJ010000129        11034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000129 DAZGTJ010000000
VERSION     DAZGTJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.129"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(137..1624)
                     /gene="yeeO"
                     /locus_tag="ACHK8E_004533"
     CDS             complement(137..1624)
                     /gene="yeeO"
                     /locus_tag="ACHK8E_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP0346708.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1785..1860
                     /locus_tag="ACHK8E_004534"
     tRNA            1785..1860
                     /locus_tag="ACHK8E_004534"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Asn,seq:gtt)
     gene            complement(1898..2848)
                     /gene="cbl"
                     /locus_tag="ACHK8E_004535"
     CDS             complement(1898..2848)
                     /gene="cbl"
                     /locus_tag="ACHK8E_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP0346709.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2950..3867)
                     /gene="nac"
                     /locus_tag="ACHK8E_004536"
     CDS             complement(2950..3867)
                     /gene="nac"
                     /locus_tag="ACHK8E_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP0346710.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4194..4269
                     /locus_tag="ACHK8E_004537"
     tRNA            4194..4269
                     /locus_tag="ACHK8E_004537"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4227..4229,aa:Asn,seq:gtt)
     gene            complement(4324..5259)
                     /gene="ldtA"
                     /locus_tag="ACHK8E_004538"
     CDS             complement(4324..5259)
                     /gene="ldtA"
                     /locus_tag="ACHK8E_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0346711.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5321..6400)
                     /gene="cobT"
                     /locus_tag="ACHK8E_004539"
     CDS             complement(5321..6400)
                     /gene="cobT"
                     /locus_tag="ACHK8E_004539"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP0346712.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6412..7155)
                     /gene="cobS"
                     /locus_tag="ACHK8E_004540"
     CDS             complement(6412..7155)
                     /gene="cobS"
                     /locus_tag="ACHK8E_004540"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP0346713.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7152..7697)
                     /gene="cobU"
                     /locus_tag="ACHK8E_004541"
     CDS             complement(7152..7697)
                     /gene="cobU"
                     /locus_tag="ACHK8E_004541"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP0346714.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7831..8268)
                     /locus_tag="ACHK8E_004542"
     CDS             complement(7831..8268)
                     /locus_tag="ACHK8E_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP0346715.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(8486..8661)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8750..9664)
                     /gene="pocR"
                     /locus_tag="ACHK8E_004543"
     CDS             complement(8750..9664)
                     /gene="pocR"
                     /locus_tag="ACHK8E_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFP0346716.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(9867..10676)
                     /gene="pduF"
                     /locus_tag="ACHK8E_004544"
     CDS             complement(9867..10676)
                     /gene="pduF"
                     /locus_tag="ACHK8E_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFP0346717.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
BASE COUNT         3122 a         2925 c         2331 g         2656 t
ORIGIN      
        1 aacagtccgc cgttctaccg actgaactac agaggaatcg tgagaacgag gcgaatatta
       61 gcgatgccca tccacaatgt caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata
      121 atctgcattt tgtcgtttat tccgacacaa ctggcttttt ttcacacttt tgcggctcgg
      181 gtcgagggta tttccacagc caacgtccag taaccattcg ccagtaaaac agcacggccc
      241 gcacagccca gtcggcaaac attcccatcc agacaccaac cacaccccag ccaagcatga
      301 ttcccagcac ataaccgact acaacccggc aaccccacat gctcaacata gaaacccaca
      361 tggcgtaacg tgcatcgcga gcccccttaa atccagcggg tagcacccat gaggcggacc
      421 aaataggcat aaataaagca tttagccaaa tcagaatcac aacgacgtgt ttaacctgtg
      481 gatcctgggt gtaaaacgat gccataaccc cggcaaaggg agccgttagc caggcgatgg
      541 ccgttaatcc aagagtggac agccagaaca catgccgcaa ctgaatctct gcttgcgcta
      601 tctgccctac tcccaacctt cggcctgtaa tgatcgtaga ggcagaaccg agcgcacttc
      661 cgggtaagtt gataagagcc gcaattgaga acgcgataaa atttccggca ataacgctgg
      721 tccccatccc ggcaacgaac atttgagtta ataaccgacc actggtaaat aacactgatt
      781 cgacactcgc tggaatacca atccccatga cttcccagat aatgctaaaa ttcagcggtt
      841 taaaatagct ctttaacgaa atccttagcg caggattaaa accaatcgcc agcacccaca
      901 aaattgcaac tgcgccaata taacgagaaa tggttaaacc caacccagcc ccgacaaatc
      961 ccagtcccgg ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa
     1021 tattcaggct accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc
     1081 cgctaccaat aagagtgatg gcagctgctg gataactgag tacggtcagc tccagataag
     1141 tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt
     1201 cgccaaaatg atgaattagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg
     1261 actgccgcgt cgccaccctc gctcgtcgtc gatctcgctt accgagacta aatgctacaa
     1321 cgacagtagt accaagatcg atagctgcaa aaaaagccat aacgaccata ttgaagctgt
     1381 ccgccaaccc cacgccggcc atcgcatctt ttcccagcca gctgaccaga aaagtgctca
     1441 ggacccccat caacaggaca caggcgttct ccatgaagat aggaacagca agcggggtta
     1501 tctcgcgcca gaacaacact ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa
     1561 caacctggcg taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc
     1621 acatttcaaa tgatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc
     1681 tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt
     1741 ttgacaaaat atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt
     1801 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     1861 aatttctgaa aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct
     1921 cttcactgtt ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa
     1981 gttccagaaa gcgccagaca tagttacgct gaagttgccc tcgcttcagt cccaaccaga
     2041 cagtattagc atcgaaaaga tgccgcgtat ccaggcggat taaattcttt tcctcttgtt
     2101 cgccgctgga ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa
     2161 tgacatcaga atcctgtgca cttaatacaa tatctgccgg caaacctttg cgggcaaatg
     2221 cgtcatcaat gcgtgagcgc cccgtaattc cctgtcggta agtaattaac ggccacttcg
     2281 ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca
     2341 aactatggtg ccagcgaaac cacgggaagg cgacgagctg cgggtcatta ctcaaacgct
     2401 cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg
     2461 taccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct
     2521 ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat
     2581 cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg
     2641 caatgaccag taatgctttg cccggttcag tcatgcccag cagtcgctta cctcgtcgaa
     2701 caaatatttc gatgccaagt tcatcctcca gttcccgaat atgacggcta acgcctgact
     2761 gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc
     2821 ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta
     2881 ttgttaaagt ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg
     2941 atataacgct tagctcacca attgccactg ccttttttcc atcactggag aactaatcac
     3001 tgacattaac aactctttca ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa
     3061 atttaaggac aaagagagac tcattgaagg agtggtaatg cgtgacatcc acccatttac
     3121 tgcgccacat aacgaacgcg cagccgattc aggtaatact gcaacgccca tgccgctggc
     3181 aatcgctgcg gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg
     3241 ccgtagggaa aaagcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa
     3301 aaggttcatt tgcgcaatag cattaacatc aacgctttgc cccgggcaat cttgagttcc
     3361 taccaggaaa agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg
     3421 ctcataaatc accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc
     3481 actattttca tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa
     3541 gggcatggtg atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga
     3601 taatgactga ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc
     3661 ccgtgcatgg gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg
     3721 aatcaaaagc tgttgattta actcaccttc cagtgtggca acctgctggc tgagcgctgg
     3781 ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta ccaatatcta caatttttac
     3841 gaagtatttc aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg
     3901 ccgccattac ttacaaccag aatgcaagat gcttgccagt tctattttag cgttgatgcg
     3961 caagctaacc aactgtcaaa taagagatta tgatagattc gtcattggcc ctttttatca
     4021 gcagtcgcat tcacctgccc tataaaagga gggtatgcac cacgatggtt cattacccaa
     4081 taagattgaa agctcaccac tttgttgaaa ttggcagcaa acaaacaaaa aaatgcattt
     4141 caccctttga catcaccatg cactgccatt aatatgcgcc ccgttcacac gattcctctg
     4201 tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca
     4261 gaggagccaa attcaaaaaa gcctgcttcg agcaggcttt ttgctttcta attaccaacg
     4321 ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgatgttg
     4381 caacgcagca tttgcgcgat ttacatcaac ttcttgcccg ttgataaacg cccgcaaaga
     4441 tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacag
     4501 cggctcatga acttccagcc actttgaacc atccggttcc gttgtgtatt tcactggctg
     4561 atcaataatt tgtacacgag tgcctacagg aacattatca aacagatatt tgatatcgtc
     4621 attgcgcaga cgaatacagc cctgacttac ccggagccca ataccaaaat tggcattggt
     4681 accgtggatg gcatacaacc tgccaatata aatcgcgtac agccccatgg ggttatcggg
     4741 ccccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt
     4801 cggcgttggc gtccaggttg gtgcttcttg tttacgttca acggtagtca cccagttacg
     4861 cggggtttct cgcccagcct ggccgatacc aataggaaag acttccactg tattactgtc
     4921 tggcggatag taataaagac gcatttcagc gacgttaacc acaatccctt tacggacagt
     4981 ggcgggcaaa atcagttgct gcggaatggt gagttgtgag ccagacttcg gcaaaaaaac
     5041 atcagcgccc ggattcgctt ccagcatatt acttaaccct tgcccgtatt gtgcggcaaa
     5101 agtctccagc ggctgagtat tgtgatcagg aacagttaca gtaagcgact gccccactaa
     5161 acggctaccc tctggaggta atggataagt taccgccagg ctattttggc tggcaaaaag
     5221 cagagcaaat gagcaaagaa tatttacacg acgcatcatg tccctttcct aagtcgcgaa
     5281 tactatccga taagtatagc ttttatcaga cttttcgttt ttagctgttc aaatcagaag
     5341 tcgtattccc cggtagaaca atattactgg cagcaagttc gcccatattg ttgtatatcg
     5401 cacaagcagc ttcgatgatg ggcatcgcca gagctgcacc actcccctca cctaaacgca
     5461 tatccatatt gagataaggc tccagcccca aatgcgacag cgctatacgc gcgccttttt
     5521 ctgccgacaa gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg
     5581 cgagcgcagc agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag
     5641 cacctaacat cactccagct attccgacca aatcaaatcc acccactttt gccaggacat
     5701 caacaccatc ccgaggattt ggttgattca acgtaatcgc ccgacgcaca acatcaattt
     5761 tattagccag tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc
     5821 ggccagtgat tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac
     5881 ctacaccaaa cagcgtgaca ccgttttttg ccagctcccg cgtataacat atgacgtcca
     5941 aaagcaactt ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac
     6001 ctcgtgcgac acgcatgttg ataagcccgg agataggctc agcagtatca ataccaacat
     6061 caattacgtg gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccgc
     6121 gggtcatatt ttcagcctgt atggctgtca cttcttttgg agaaatagcg accccttcct
     6181 cccagacgcc gtgatcggca cacataacca gtatcgcttt tttgcccaca tgcggtatgc
     6241 cattcaaccc cggcattcct gccagttgta tggcaagcgc ctccagcttt cccaggctac
     6301 caacaggttt gagtaacccg tcaatatgcc gttgtgcacg cgacatagcg gcaggatcga
     6361 tggctgggat cgtattcagt aaatcggcaa gtatttgcat gtcacgtctc tttatagcag
     6421 tgccagcagg aaaaccagtt caccaagctc tatggctgct cccagcgtat cgcccgtttg
     6481 cccgcccagc gtacgtttga gaagctgacc gaggatgaaa atcaccacca tcgtcaccac
     6541 catagcagcc acaccatgca tacccggcaa taatacggca gcaaaaatag cggctaagcc
     6601 gagtgtgacg caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc
     6661 ttcccgcgca taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca
     6721 tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gtcaactcgc ttaacaccag
     6781 aatctttacc agtaccacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc
     6841 acgcatgatt tccaacatgc gatcgcggct acgtgcagaa aatacgccat cacaggtatc
     6901 agcgaggccg tccagatgga atcccccggt catcagcgcc agcacaagta cgctaaacag
     6961 tgccgccagt ggtacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc
     7021 gccaagcaat aatccaatca aaggaaaagt aataataccg cgagaataat gctcgaaatc
     7081 cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaatgaga gcatcgccca
     7141 aaataattta ctcatttgat ttttactcca atacccgaaa ccaccagcca tacttcattt
     7201 gccgcagcgg ccaactgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga
     7261 cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg
     7321 caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc
     7381 tgataattcc attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc
     7441 attgtggtaa cgcattccag caacacagcc tcattcgggt taatgtctgc atgaattaat
     7501 tcatcaagat gttgccaacg ctccactgtg cgccagtgct ccgggcggcc ttgccgatga
     7561 tgttctatcc gtgcagccat ctcatcatca aggatttgcg aggtggcgat atacagaacc
     7621 tgtgaagagt ccccaataag cgcctctgcg tggcgactct tcccgctccg tgcgcccccc
     7681 gtcaccaaaa tcatcatacc gactcctgat tcaattgtat gatggtattc attgaacaga
     7741 acaatggatc ccccaccgtt gctgaccgca aaacatttac gcgattaata ctgcgcgtaa
     7801 tataattaaa aatccagcat tctcaatcca ttacgaaaga agatggcatt gcggaatagc
     7861 cagacttcct cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt ctctggcatg
     7921 acagatttca ttcagataat ggatcaatgc cacagcccca ccagccggac cagtcagcaa
     7981 acgggcattg tgttctgcaa tcacaattac cgtcgttccc tcagaatgag ggagccattc
     8041 tggtacggat atctcgcttt cagctgctat caatagcagc actgaataat cgctatacgc
     8101 atctgataat gattgcacag acccaccgtt aagaggctgg aaatcttgct gccagtcctc
     8161 accaacaaaa caggttattg gcaatatttc agccagatgt ttcgccaggt ccacatctcc
     8221 agataagagg caaaaaagag ctatcgattc gggttttatc gttttcatgc ctctcgccct
     8281 ccacttagtt tcctgtaatg tcggtctggt tgatgagcca tgaaacaaga ttgccacacc
     8341 ttaagtgcag ccctggtgcc tgtcggcact gcgatgaaag aactaaatga tgccgccata
     8401 ccctgatcct ttcagggtgg taggggtata aagacgcgcc ggtactctac cggacgcttc
     8461 cgggcaaccc acttcccacc gaagttgttg gtattgactg acagactggt cttctggctt
     8521 agcgtcatcc tcccccgacc ttcccaatct cgcgatcagt ggtattttcg ggtttgtcag
     8581 cattacagca gcgggggctg cgagggattt tcaccctctt cccaggcaca aatgtgccac
     8641 acctgtcagc gtatgttatg agctgatttc acttttccca taacacgtaa ataacgttaa
     8701 ttattcattc cagcgtttat ttaaaccttc ccttttttta gcatttactc tataccgttt
     8761 ccatcgccgc attttcactc acttgctgtc gatacacctg cggcgtaacc tgataagtct
     8821 gacggaaaac tttgcaaaaa taactggttt gcgaaaaacc taaattacgc gcaatgctgg
     8881 cgatgctcca gtcactatgg cacagtaact cacgagcgct ggccatccgt tgttggttta
     8941 cccacgcatt aaaaccgatt ccatggtatt tcttgaataa cttactgaaa taataaggac
     9001 ttagataaac gtgcgaggcg acatcttcca gacgcaaatc gtctgataaa tgcgcatcaa
     9061 tatagcgtaa agcttttttc attttattgt cgtggggatt aggcgcacga gtctggcgca
     9121 ccgaatctga ctgagatttg ttctctttaa taaccacaaa attgagttgt tttttgaggc
     9181 aattttccac aatcaacttc aacaaatcag cagaggccat aactcttgag taatccatct
     9241 caggaacttt gcggaattca ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat
     9301 tcagaatatc gaccaaatcg acatcatcat tacgcagacg aacttgcccg cacaatacaa
     9361 aaccaactaa atgtccggcg attaccagtg gaatagagaa atcggttaat ccggcatgac
     9421 agcgataaat acaaggctga tcggatttag aggcttccag acccccacaa cgatcgctca
     9481 tgcgacatcg agtgctcagc tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt
     9541 taaacagctc ggaaatttca tcgccgtgaa tattgaccac gacaaccgca aggctggtcg
     9601 cctgcgcaaa atcctgcgcg attttattaa tgagttctga attaagtgtc gccgcagaaa
     9661 tcatgataaa acctcccagt taatttattg ttatagcaaa acaattgttt tacatttaat
     9721 cttcacatca tccttgatga tttactgacg agaagcacaa aggacaataa caaaagcctc
     9781 cgtgaagcgg gagacttttt aataagttcg gaaagcagat cacaaacagt aaaaaaataa
     9841 acgtttataa ctgccagatt atttcgttat tcgctgattt ctttaggcgt tacactattg
     9901 acaggtaaat tctttgcaat gagatagcga taaatcgccg caccaacaca tgccccaatg
     9961 atcggtgcaa taatgggtac aatgaaataa ggaatattgc gtccaccagt catcgcaatt
    10021 tccccccaac ccgcaaacca ggtgaaaatt ttaggtccaa aatcacgcgc tggattcatg
    10081 gcaaaaccgg ttaatggtcc ggtagaagca ccaataacag caaccagaat accaatgagt
    10141 aaaggcgcaa gcggtccttt gggtacgcca ttaccgtcat cggtcaacgc cataatcaac
    10201 cccattaaca tggacgttat gaccacttcg accaacgctg cttgccatac attaatcgcc
    10261 gctgctggat aagtactgaa gatactggcg agttgtaaac tttctgcgct accacgcact
    10321 atattgtgag cagattcaaa atcggtaaac aaactgctgt agagagaata tgccagaacc
    10381 gcaccaccaa aggcacccgc taattgcgcc acacaaaatg gcagcacttt acgggctggg
    10441 aaagaggcaa acagccacag agcaatggta actgccgggt tgagatgagc gccggaaata
    10501 cctgccgtaa ggtaaacagc aagcgaaatc cctaatcccc aaatgataca aatttcccac
    10561 agtcccagac ttgccccggt aaccttcagg gcgcacaaac aaccaatgcc aaaaaagagg
    10621 aacagtccgg tgcccaaaaa ctcggcaacg cattgcgcct tgagtgaatc attcatagtg
    10681 acaccttctt tacggagttt ttctttatca gccaactatg cgctgattgt tgtgtatacc
    10741 gagcgcaact ataaaaaatt gctctgcaga aaatattgcc aacatgcatg acagactcgt
    10801 gtaaaaatcg tgctaacata acaaattatt gtcattgccg aatcatgcaa cacagcgtta
    10861 atcagtgttg aaagtaataa tttccaccaa acttttacaa taaaaaattg ccatcatgac
    10921 ataataaaat tgaaacaaat atcacagttc cttccttcga accaaactca acgaaatatt
    10981 actcgcgatt aaactcacta gccaacgcaa ttaattcaca ttgaaatatc taca
//
LOCUS       DAZGTJ010000130        10923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000130 DAZGTJ010000000
VERSION     DAZGTJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.130"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            93..854
                     /gene="adiY"
                     /locus_tag="ACHK8E_004545"
     CDS             93..854
                     /gene="adiY"
                     /locus_tag="ACHK8E_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP0346718.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            991..2328
                     /gene="adiC"
                     /locus_tag="ACHK8E_004546"
     CDS             991..2328
                     /gene="adiC"
                     /locus_tag="ACHK8E_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP0346719.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGLFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2432..4075
                     /gene="eptA"
                     /locus_tag="ACHK8E_004547"
     CDS             2432..4075
                     /gene="eptA"
                     /locus_tag="ACHK8E_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP0346720.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4072..4740
                     /gene="basR"
                     /locus_tag="ACHK8E_004548"
     CDS             4072..4740
                     /gene="basR"
                     /locus_tag="ACHK8E_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP0346721.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4741..5841
                     /gene="pmrB"
                     /locus_tag="ACHK8E_004549"
     CDS             4741..5841
                     /gene="pmrB"
                     /locus_tag="ACHK8E_004549"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP0346722.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5817..5906)
                     /gene="pmrR"
                     /locus_tag="ACHK8E_004550"
     CDS             complement(5817..5906)
                     /gene="pmrR"
                     /locus_tag="ACHK8E_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP0346723.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6018..7520)
                     /gene="proP"
                     /locus_tag="ACHK8E_004551"
     CDS             complement(6018..7520)
                     /gene="proP"
                     /locus_tag="ACHK8E_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP0346724.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7784..8662)
                     /gene="rdcB"
                     /locus_tag="ACHK8E_004552"
     CDS             complement(7784..8662)
                     /gene="rdcB"
                     /locus_tag="ACHK8E_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP0346725.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8659..10888)
                     /gene="crfC"
                     /locus_tag="ACHK8E_004553"
                     /pseudo
     CDS             complement(8659..10888)
                     /gene="crfC"
                     /locus_tag="ACHK8E_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
BASE COUNT         2750 a         2857 c         2666 g         2650 t
ORIGIN      
        1 gtttatattc atcatacaat tatataacca tttcccggta tcgctttgct ttagcgagaa
       61 ctggtctttt attcgcactc aggagtacat gtatgaggat ttgcagcgac caaccttgta
      121 ttgttttatt gactgaaaaa gatgtctgga taagggtgaa tgggaaagaa cctattagcc
      181 ttaaagctaa ccatatggcg ttattaaatt gtgaaaataa tattatcgac gtctcctctc
      241 ttaataacac tttggttgct catattagtc acgacatcat caaagattac ctccggtttc
      301 tgaataaaga tctctcgcaa ataccagtat ggcaacgtag cgctacgccc atcctcaccc
      361 ttccatgcct gacgccagac gtctttcgcg ttgccgcgca acatagcatg atgcccgctg
      421 aaactgagtc agaaaaggaa cgaacacgtg cattattatt cactgtgcta tcccgttttc
      481 tcgacagtaa aaaattcctt tcactaatga tgtatatgtt acgtaattgt gtaagtgaca
      541 gcgtttatca aattattgaa agcgatatcc acaaagactg gaatcttagt atggtagcca
      601 gttgtttatg tcttagccca agtctgttaa agaaaaagct gaaaagcgaa aacaccagtt
      661 atagccaaat aatcaccacc tgccgcatgc gctatgccgt aaatgaatta atgatggacg
      721 gtaaaaatat ctctcaggta tcacagtcct gcggctacaa cagtacgtcg tactttattt
      781 ctgtctttaa agacttctac ggtatgacgc cgctgcatta tgttagtcag cacagagaac
      841 gcactgtcgc ctgattttta accttaacga agagctatat taataacggc atcagcgata
      901 acccggccga taataattca actatcgaat gcaggcgtat gatacgacgt aatttattgt
      961 cacgaagctc gccttcgcag gagtttaatt atgtcttcgg atgctgatgc tcacaaagtg
     1021 ggcttaatcc ccgtcaccct gatggtgtcg gggaatatta tggggtcagg tgtttttctg
     1081 ttacctgcaa acctggcctc cactggcggg attgccattt atggatggtt ggtgacgatt
     1141 atcggtgcgc tggggctctc gatggtatac gccaaaatgt cgttcctcga cccaagtcct
     1201 ggtggttctt acgcttacgc ccgccgctgc tttggcctgt ttctcggtta tcaaaccaac
     1261 gtcctctact ggctggcctg ctggatcggc aatatcgcca tggtggtcat tggcgtagga
     1321 tatttaagtt acttcttccc gattctgaaa gacccattgg tattaaccat cacctgcgtc
     1381 gtggtgctgt ggatcttcgt cctgctgaac attgtcggtc cgaaaatgat cacccgcgta
     1441 caggccgttg ctaccgtgct ggcgctgatc cccatcgtcg ggattgccgt atttggctgg
     1501 ttctggttcc gtggtgaaac ctatatggcg gcatggaacg tcagcggtct gggcaccttc
     1561 ggtgcgattc aaagtaccct taacgttacg ctgtggtcgt tcatcggtgt ggaaagtgcc
     1621 tccgttgccg caggtgtggt gaaaaacccg aaacgcaacg tccctatcgc caccattggc
     1681 ggggtattga ttgctgccgt ttgctatgta ctttctacca ccgcgattat ggggatgatc
     1741 cctaatgccg cactgcgcgt ttctgcttcg ccattcggtg atgccgcacg gatggcgttg
     1801 ggtgacaccg ccggggccat tgtttccttc tgcgcagctg cgggttgctt aggttcactg
     1861 ggcggctgga cgttgctggc gggtcaaacg gcgaaagccg ctgccgatga cggactgttc
     1921 ccaccgattt ttgcccgtgt taataaagcg ggcacgccag tggcggggct gattatcgtc
     1981 ggtattttga tgaccatctt ccagctcagt agcatttcgc caaacgcgac caaagaattc
     2041 ggtctggttt cttccgtgtc ggtcatcttt acactggtgc catatcttta cacctgtgcg
     2101 gcgttactac tgctcggcca cggtcacttt ggtaaagcac gcccggcata cctggcagtg
     2161 actaccattg ccttcctcta ctgcatctgg gccgtggtag ggtccggcgc gaaagaggtt
     2221 atgtggtcat ttgtcactct gatggtaatc accgccatgt atgcgctgaa ttacaaccgg
     2281 ctgcataaaa acccgtatcc cttagatgca ccaataagca aagattaatt ccccttaatc
     2341 aagcaaacat aaaagccaac cttaagaact taaggttggc ttaattttgc tttgcgagca
     2401 tatgcgcact ttgttcgatg gaaacaccgt gatgttgaag cgcctactaa aaagaccctc
     2461 tttaaattta ttctcctggt tattacttgc tactttttat atctcagtct gcctgaatat
     2521 cgcctttttt aaacaggtgc tacaggtgtt accgctggat tcactgcata acgtgctggt
     2581 tttcttgtcg atgccggtcg tcgcattcag cgtgattaat attgtcctga cactaggctc
     2641 tttcttatgg cttaatcgtc cactcgcctg cctgtttatt ctggtcggtg cggctgcaca
     2701 atatttcatc atgacctatg gcatcgtcat cgaccgttca atgatcacca atatcattga
     2761 tactacgccg gcggaaagtt atgccctgat gacgccacga atgttattaa cgctggggtt
     2821 aagtggcgtg ttagccgcga ttattgcctg ctggatcaaa atcaccccca ccacctcacg
     2881 cctgcgcagc gttcttttcc gcggggccaa catcctgatt tctgtactgc tgattctgct
     2941 ggttgccgca ctgttctaca aagactatgc ctcgctgttt cgcaacaaca aagagctggt
     3001 gaaatcctta agcccgtcca acagcattgt tgctagctgg tcgtggtact ctcaccagcg
     3061 gtcagccaac ctgccactga tacgcattgg tgaagatgct caccgcaacc cattaatgca
     3121 aaatgggaaa cgtaaaaacc tgaccatttt gatcgtcgga gaaacgtcgc gcgcagagaa
     3181 tttctcgctg aatggttatc cacgcgaaac caatcctcgt ctggcaaaag ataacgtggt
     3241 ctatttccct aataccgcct cttgcggcac ggcaacggca gtttcagtac catgcatgtt
     3301 ctcggatatg ccgcgtgagc actacaaaga agagctggca cagcaccagg aaggcgtgct
     3361 ggatatcatt cagcgagcgg gcatcaacgt gctgtggaat gacaacgatg gcggctgtaa
     3421 aggcgtttgc gatcgcgtac ctcaccagaa cgtcaccgcg ctgaatctgc caggtcagtg
     3481 catcaacggc gaatgctatg acgaagtgct gttccacggg ctggaagagt acatcaataa
     3541 cctgcagggt gatggcgtga ttgtcttaca caccatcggc agccacggtc cgacctatta
     3601 caaccgctat ccgccgcagt tcaggaaatt taccccaacc tgtgacacta acgagatcca
     3661 gacctgtacc aaagagcaac tggtgaacac ttacgacaac acgctggttt acgtcgacta
     3721 tattgttgat aaagcgatta atctgctgaa agaacatcag gataaattta ccaccagcct
     3781 ggtttatctt tctgaccacg gtgagtcgtt aggtgaaaat ggcatctatc tgcacggtct
     3841 gccttatgcc atcgccccgg atagccaaaa acaggtgccg atgctgctgt ggctgtcgga
     3901 ggattatcaa aaacggtatc aggttgacca gaactgcctg caaaaacagg cgcaaacgca
     3961 acactattca caagataatt tattctccac gctattggga ttaactggcg ttgagacgaa
     4021 gtattaccag gctgcggatg atattctgca aacttgcagg agagtgagtg aatgaaaatt
     4081 ctgattgttg aagacgatac gctgttattg cagggattaa ttctggcggc gcaaaccgaa
     4141 ggctacgcgt gcgatggcgt gacaaccgcg cggatggcgg aacaaagcct tgaggccggt
     4201 cattacagcc tggtggtact ggatttaggg ttacccgacg aagatggact gcattttctc
     4261 gcccgtatcc ggcagaaaaa atacaccctg ccggtactga tcctcaccgc tcgcgatacg
     4321 ctgaccgaca aaatcgccgg gctggatgtc ggtgccgatg actatctggt gaagcctttt
     4381 gcgctggaag agttacacgc ccgtatccgc gccctgctac gacgccataa taatcagggc
     4441 gaaagtgagc tgattgttgg caatctgacg ctgaatatgg gtcgccgtca ggtatggatg
     4501 ggcggtgaag agttgattct gacgcccaaa gaatatgctc tgctgtcacg gttaatgctc
     4561 aaagcaggca gtccggtgca tcgggaaatt ctctacaacg acatctataa ctgggacaat
     4621 gaaccctcga ccaacaccct ggaagtgcat atccacaatc tgcgcgacaa agtgggcaaa
     4681 gcccgtatcc gcaccgtgcg cggctttggc tatatgctgg tcgcaaatga ggaaaactaa
     4741 ttgaatctga tgcgttttct gcgccgacca atatcgctgc gccaacggct gatattgacc
     4801 attggggcca ttttgttggt gtttgagctg atcagcgtct tctggctatg gcatgaaagt
     4861 accgagcaga ttcagctgtt tgagcaggcg ctacgcgaca atcgcaacaa cgatcgccac
     4921 atcatgcgtg agatccgcga ggcggtcgcc agcctgatcg tccccggcgt ctttatggtc
     4981 agcctgacgc tatttatctg ctatcaggcg gtacgccgca tcacccggcc gctggcggag
     5041 ctgcaaaaag agctggaagc gcgcaccgcc gacaacttaa cgcctattgc cattcacagc
     5101 gccaccctcg aaatcgaagc ggtggtttcg gcgttgaacg atctggtcag tcgcctgacc
     5161 agcacgctgg ataacgaaag gttgtttacc gctgacgtcg cgcacgaact gcgaacgcca
     5221 ctggcggggg tgcgtttgca tctggaactg ctggcgaaaa cgcatcacat tgatgtagca
     5281 ccgttagtgg ctcgacttga tcagatgatg gagagtgtct cccagctgct gcaactggcg
     5341 cgtgccggac agtcattttc ttccggtaat tatcaacatg taaaactgct ggaagatgtc
     5401 attctcccct cgtatgacga actcagtacc atgctcgacc agcgacagca aaacctgcta
     5461 ttgccagaga gcgccgccga catcaccgtt cagggtgatg caaccctgct gcggatgtta
     5521 ttgcgaaacc tggtagaaaa cgcccatcgt tacagcccgc aaggcagcaa cattatgatt
     5581 aagctgcaag aagatggcgg agcggtcatg gccgttgaag atgaaggacc gggtattgat
     5641 gagagtaaat gcggggagtt gagtaaagcg tttgtacgta tggacagccg ttatggcggg
     5701 attggtctgg ggttaagtat tgtcagccgc attacacagt tgcatcacgg gcagtttttc
     5761 ctgcaaaacc ggcaagagac ttccggcacg cgggcctggg tacggctgaa gaaagatcag
     5821 aacgtggcaa accagatata aagaaagctg ctgaccacca gcacgctgaa cacggtagtt
     5881 aaactttcat aaacacggtt tttcattgca ctctcctccg ttaacctgga ggagagtatg
     5941 cgcgtcagag attaagtgaa ccttaagagt tcaattggcc tgataagaca gcgtcacatc
     6001 aggccatccg tttcagctta ttcatcaatt cgcggatgtt gctgcaccag gcgagtacgt
     6061 ttcgcctgca aatcggcaat ctcgtggtca atatcatcga ttttctgctc gatattatcg
     6121 taatgctcga cgagaatttc cttcgcttcc tgtatatctg acgccgccgg tgtcgcacct
     6181 ttcaacggac gatttgccgt ctctttcatg gttacgccgg tgattaaacc aaccaccgcc
     6241 actaccatca ggtaataggc aggcatcatc agattctgcg agctttcgac cagccaggcc
     6301 gccagtgttg gcgtcagacc ggcaaccagc accgaaatat taaatgccgc cgccagcgcg
     6361 ctgtaacgga tatgcgtcgg aaacatcgct ggcaaggtag aagccataac gcccgtaaag
     6421 cagttaagga tcaccgccag catcagtaac ccggcaaaaa tcaggccgat gacgttactg
     6481 ttaatcagaa taaacgccgg gatcgccaac acaaacagtg caacactacc aagtagcaca
     6541 aacggacgac ggccaaaacg gtcactcagc aagcccatca ccggctggac aaacagcata
     6601 ccgatcataa tggcgataat aatcagcacc ccgtggtctt cggaataatg caggttatgc
     6661 gacaaataac tcggcatata ggtcagcaac atgtagtaag tcacgttggt ggcaattacc
     6721 agaccaatac atgtcaacag gctgcgccag tgtttggtgg caatctcttt aaacgagact
     6781 ttcgggccat cctgcaaacc ttcgcggtcg ccctgctcca gtttatcaac atgctgctgg
     6841 aacgccgggg tctcttccag cgcatggcgc aggtaaagcc cgataatccc taacggcaga
     6901 gcaataaaga acggaatacg ccagccccag tcgaggaagt tcgcttcgcc gacaatggtc
     6961 gaaattaaca ccaccacgcc cgcacccagc acaaacccgg caatagaacc gaagtccagc
     7021 cagctgccca taaagccacg tttacggtca ggggagtatt ccgcaacaaa tatcgacgcc
     7081 ccggtatatt caccgccgac cgagaaacct tgtgccatct tacagatcaa cagcagaatc
     7141 ggtgcccaaa taccaatcgt gtcgtaggac ggtattaagc caatacagaa cgtactgatc
     7201 gacataatca caatagtgat agcgaggatc ttctggcgac catatttatc gcccaacata
     7261 ccaaagaaga gtccaccaag cggtcgaatc agaaagggaa cggagaaagt ggcaagtgca
     7321 gcaaccatct gcacgctggg gtcagccccc gggaaaaaaa ctttacctaa tgcgtaagca
     7381 acaaaaccat aaacaccaaa atcaaaccat tccattgcat tacccagtga tgctgcggta
     7441 atggctttac gcagtttacc gtcatcaata atggtgacat cacgaagggt aattggtttt
     7501 acttttttcc ttttcagcat agctttcctc gcagagttgg cccatttccg caaacgccag
     7561 aaaaatcagc gcgacggccg caaaagggga gcgggaattg caggatgcaa tctctgtaac
     7621 aagcgtagcg ggtttgctta cactctccgg ttaaggaatg tacaatcaaa ccagttgacg
     7681 cctgaattgg gcagttaatg acctttttac cctaccagcg tttaaatttg tgatcaactt
     7741 cacacatgga tttcgtttag gactcattga tgtaactgat tatttacacc aaatttacag
     7801 agaagatttc ctcgaccatt gcccccgcca gacgctgcgc tgaacagagt cgtggcatcc
     7861 ccagtgcgac agttcgccag cgttgggtca ctgaccagca aacgggttgt ttatctgccg
     7921 tggaccactt tcccagccaa cttaaaaaat cagcatgatc cccgcccgtc ggaatggtcg
     7981 aagaggcaaa ccactgatgg taatagtgac gcgtggtcgg ggccgcattg atactctctg
     8041 ccagatgttg tgtcgctttc acgcgcaaat cacccagcaa caacgccacc gcttgcgggc
     8101 ttcccagacg aaaggcatcg ccaaatgccc cccaacgcaa ctcctccaga acgataaaac
     8161 agcgcccagg caacgaccag gcgttgtacg ccccagcacg ccagcgggta aaaatttgct
     8221 cgctctgttc acccgcctgc accagtaggc ctcgttgcgt taacgccttt tccttccagc
     8281 gacaacgggc tgaaaaccac tcatacaagg cggcaatttg ctgggggagc tcagggaaag
     8341 cagagtgctg gcggtgatcc attttttgca gcagcgccag atgttgcgcg atctgcgtca
     8401 gcgccagatg accgggatcc agcatcgacg ccagtttttc caccaatgtc agacgcaggc
     8461 taagcggcgc atttaacatc ccgccgttcg tccatgcctg aagctgcaca cgcgcaagaa
     8521 gttcctgcat cagtcgttca cgaaactgct gctgatgggc ttgtggatgg cgggcatcat
     8581 cgccctgcgc cagatccact aaaaagcggg gatgaatcga ctccagcacg cgaccggggc
     8641 cgtccagtaa cgtcttggtc aatatcgttc tgccgtgaaa agtgtttgaa tatcatcgcg
     8701 taacagctga gcgtcggtgt aaatccagcc atgagtcatc acagtctgct gcaattgctg
     8761 ctgcatcagc ctgaccaccg attcattttg ttgacgcaga gccagacttt cgcgtaaacg
     8821 cgtctgtaat tccgtcaaac atgaagcgaa ctcagcgaaa aaagtattca tgcctgccgt
     8881 aacagattca tcgacctgct ctgccagaac tttagccatt tgttggcaat aaagatcgac
     8941 ttctgcgctt aatgctcgtt gcaacgcact gtaatcaacc gtctctatcg gggatttctc
     9001 atttccccgt ccccagtcgg gctgattcaa ccagcgcgaa aaagtctcac gcacaacgcc
     9061 taaacgcgtg ctctgctcgt ccattgcgtc ctggcgcgaa atgacttcac tgaacagctg
     9121 gcgagtgttg aagtggggaa ctacgccgtg aaaaacagga aaatgaaacc caggacgaaa
     9181 ccctgactcg ctcaattcca ttttgatttg ttgctcaatg gggcgaataa catcggttaa
     9241 cacccgggca aagggtggat tccagctcgg caaaacgcag cgtaaagtcg cgactaatgg
     9301 tgttctgcgc catctgtaac agtgtctcac agcaggtacg aatctcgctt aatgaccctg
     9361 aatcatcctg aaacaaggcg gctaactgcg cattcagcgc atcttgctgt tgacgcagaa
     9421 agtggttggc ggaggtcagg gccagctcga tttcatgttt aatctcgccg ctcacctgcg
     9481 cctgattgag ttgcaatagc tgcaaacttt cttcgatctg atggatattt tgccgcaatt
     9541 gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc gcctgctgcg
     9601 cgtaattcaa cagtttatgc gcggcagatc gcagagcata caacgaggcg ttagcgtaag
     9661 cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac aactgatcgg
     9721 cagcatgacg aatatgttcg aggtccgcca gatcggcatg acgccagcgc ctgccgagcg
     9781 cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cggtggtaac ttaccgttgt
     9841 tggctaactc atggcgcacc cgattcgcca ggtagcccca catcgacgac accggaaata
     9901 tctgctgtgg cgtaatacaa cctttcatca gcgtcccgga aatcagtgcc cgcacctggt
     9961 cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca tacaacggca
    10021 ccgattgccc caccgccaaa attgcctcac ggacctcttc atcggagatc gatttcagtt
    10081 gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg ttaagcattt
    10141 tttgcagatg cggttgcccg gcttcatttg gcccgggggt atccagtaac gtcaactgac
    10201 cgggataact ctccagcccc gccagatgga caaactccac ttcaatcacg ggaatatgct
    10261 caatggcggc gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc agtcgcacca
    10321 aatcattcaa acttttcaga caatgaaaaa taggttgagc acccagataa tatttttcga
    10381 aagcgatgcc attttcaatc cgctgcataa gcgcacgcat atctttatct atttccagca
    10441 catcggtcag atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt tgaattaaat
    10501 ggtcgattgg cgcgacatgt gaaaaatgca gtaccggttc tttttgcccg ggcgtatggc
    10561 gaataagcgt tggcagcgca gtcattgggc gattacgatt aggcagaacc tccgtaccaa
    10621 caatggcatt aatggtggtt gatttccctg ctttcatggt accgacaatc gccagcacca
    10681 tttccagccg ggaaatttta cgcaactcat tattcagcat cgcgtgacgt tcggcgatat
    10741 taggctgact ccagggtaaa gccagttgtg gcgtatcgtc tccgggtaca gagagaggta
    10801 ttttttccag taactgcaac tgttggcgag aaagctgtaa caggcgttca gcctcctgac
    10861 ttaactcata cagggtctgt gtatacatag aatattcttc cttaaagcaa attttattac
    10921 ttt
//
LOCUS       DAZGTJ010000131        10892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000131 DAZGTJ010000000
VERSION     DAZGTJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.131"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(138..1802)
                     /gene="glnS"
                     /locus_tag="ACHK8E_004554"
     CDS             complement(138..1802)
                     /gene="glnS"
                     /locus_tag="ACHK8E_004554"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP0346726.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2005..3951)
                     /gene="nagE"
                     /locus_tag="ACHK8E_004555"
     CDS             complement(2005..3951)
                     /gene="nagE"
                     /locus_tag="ACHK8E_004555"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP0346727.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4284..5084
                     /gene="nagB"
                     /locus_tag="ACHK8E_004556"
     CDS             4284..5084
                     /gene="nagB"
                     /locus_tag="ACHK8E_004556"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0346728.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5144..6292
                     /gene="nagA"
                     /locus_tag="ACHK8E_004557"
     CDS             5144..6292
                     /gene="nagA"
                     /locus_tag="ACHK8E_004557"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0346729.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            6301..7521
                     /gene="nagC"
                     /locus_tag="ACHK8E_004558"
                     /gene_synonym="nagR"
     CDS             6301..7521
                     /gene="nagC"
                     /locus_tag="ACHK8E_004558"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP0346730.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7569..8321
                     /gene="nagD"
                     /locus_tag="ACHK8E_004559"
     CDS             7569..8321
                     /gene="nagD"
                     /locus_tag="ACHK8E_004559"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP0346731.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8577..10241
                     /gene="asnB"
                     /locus_tag="ACHK8E_004560"
     CDS             8577..10241
                     /gene="asnB"
                     /locus_tag="ACHK8E_004560"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP0346732.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10622..10698
                     /locus_tag="ACHK8E_004561"
     tRNA            10622..10698
                     /locus_tag="ACHK8E_004561"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10656..10658,aa:Met,seq:cat)
     gene            10707..10791
                     /locus_tag="ACHK8E_004562"
     tRNA            10707..10791
                     /locus_tag="ACHK8E_004562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10741..10743,aa:Leu,seq:tag)
     gene            10815..10889
                     /locus_tag="ACHK8E_004563"
     tRNA            10815..10889
                     /locus_tag="ACHK8E_004563"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10847..10849,aa:Gln,seq:ttg)
BASE COUNT         2703 a         2804 c         2779 g         2606 t
ORIGIN      
        1 atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaaa cgccgcagag
       61 cgcggcgttt gggattgttt gcatatttct tgccggatgt ggcgtaaatg cctcacccgg
      121 cctggtagaa cttaaaacta ctcgcctact ttcgcccagg tatcacgcag ccctacggtg
      181 cggttaaata ccggtttttc ggcagtggaa tggcggctgt cgaggcagaa ataaccttca
      241 cgctcaaact ggaatgcttt acccgctacc gcgtctttca gcgacggttc agcgaaaccc
      301 tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg
      361 ttaggcacgc tgaacagacg gtcatacaaa cggatttcaa ccggcagcgc atgtgccgcg
      421 ctcacccagt gaataacacc tttcacttta cgaccatctg ccggatcttt gcttaaggtg
      481 tcggcgtcat aagtacagaa gatggtggtg atattacctt cggcatcttt ctcgacgcgt
      541 tctgccttaa tcacgtaagc attacgcaga cgcacttctt tacccaacac cagacgtttg
      601 tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc acagctaaac
      661 ggcacctgac ggctacccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg
      721 ccttcgccct ggtagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc
      781 ggggcatttt cgttgagatc ttcacggatg caggattcca gtgacgccat ctcaatggtg
      841 ttgtcctgct tggtcacacc aatgcgtttg cagaactcac gaatagaagc cgcagtgtaa
      901 ccacgacgac gcagaccaga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc
      961 ttatcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg
     1021 cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga tgttgtccag tacccagtcg
     1081 tacagacggc ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc
     1141 gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc
     1201 tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc
     1261 gacgccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaact accggtacgc
     1321 attttttcga acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta
     1381 cccggctgcg tcagggtgcc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg
     1441 tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg
     1501 gaggagtaac ggacgttacc agaccagtga aaacctaacc actcaacgtc gtttttgatc
     1561 gactcaacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac
     1621 tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata
     1681 tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg
     1741 gccagatctt catcgatgat ctgacgaata aagttagtcg ggcgggcttc tgcctcactc
     1801 atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca
     1861 ggctgacaac tgttagttag tgaaaatcga aaatctcact gagattatgg ggatgattga
     1921 tgaaaaaaaa agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctcaa
     1981 gcgtcgcatc aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc
     2041 cacaacatgg ccctgagctt taatgatcag gccgctgaaa tcgtcgatat tgctgcaaac
     2101 caccgggcta atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat
     2161 cggttgtcct gcgcttacct gcgcgccctc ttccaccaga cgtttaaagc ctttaccttc
     2221 cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag
     2281 acagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac
     2341 gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc
     2401 gtcaggaacc tgatccagtg ccacaacatc accggtaatc ggcgatacca gctccgcgat
     2461 agacaccgcg tttggtacag cctgcggttt tgctacaggc gcggcagttg caggcgcagt
     2521 ttcagctgac gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat
     2581 ggattctgct ttcgcgccca caatcacctg aatagtctgt ttgttcagtt ttactacccc
     2641 agaagccccc agacgtttac acatcacatc gttgacgcga gctgagtcaa ccacggtaag
     2701 gcgcagacga gtaatacagg cgtcaatggc tttcaggttg tcagtaccgc caaccgcagc
     2761 aatatagttg gttgccagtt gattgagacc ttcttccgtg ttgctgttgg cttcttcagt
     2821 aacgatctcg tcttctttat cttcacggcc cggcgttttc aggttgaaca tgcggataac
     2881 caaactgaac accacgaagt agatagcgaa gaagacaaca cccatcacca gcagcatcca
     2941 gacgttctgg ctggcggccg gcaggttata catcaacgcg tagtcgatag cccccgcaga
     3001 gaaggagaag cccgcgtgga tacccagcag cgttgccaca aacaggctga taccggtcag
     3061 cagtgcgtgc aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc
     3121 agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcataccgc caaccatcgg
     3181 acgacgctct ttcggtgctg caaagtacat cgccagcgcc gcacccggca gaccgaacat
     3241 catgatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccgg catagaagcg
     3301 gttaatgtcg ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca
     3361 ggcgatggtg ttcagtacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc
     3421 aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc
     3481 gtgctgtacc ggcggccaga cgtaaccaaa aatggccgcc agcaccaggc agaagaagcc
     3541 ggtggcaatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat
     3601 atcggaccaa cggttatagg ctgcaccacc gaccagaccg gtaatgatac ccgccagtac
     3661 acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac
     3721 cgcacccgcc agtgccgccg cacctgcgct gtctttcgac cagctggatg ccacgccgat
     3781 ggcgaagatt aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa taaacgcaac
     3841 gttaagtaaa tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat
     3901 agggagctgt aacgccctac ctagtcgctg gaaaaaacct aaaatattca tcttattccc
     3961 cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattgtac
     4021 tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat
     4081 tcgtatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc
     4141 actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat caggtcggat
     4201 tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc caacggctta
     4261 cattttactt attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg
     4321 gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg actgccgatc
     4381 gtccgtttgt actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag
     4441 tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg
     4501 aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt
     4561 tcttcgatca cgttgatatt ccagcagaaa atatcaacct tctcaacggc aacgccccgg
     4621 atatcgacgc cgaatgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc
     4681 tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc
     4741 tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt
     4801 tctttgataa cgatgttaat caggtgccaa aatatgccct gactgtcggt gttggtacat
     4861 tgctggatgc cgaagaagtg atgattctgg tgctgggtag ccagaaagca ctggcactgc
     4921 aggccgccgt tgaaggttgc gtgaaccaca tgtggaccat cagctgtctg caactgcatc
     4981 caaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa
     5041 gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattatta tccctgccct
     5101 ggctccttgc tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca
     5161 gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg
     5221 cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact
     5281 gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt
     5341 gcagtttaac gatactgctg aagcagtcag cgtagaaacg ctggaaatca tgcagaaagc
     5401 caatgagaaa tcaggctgta ctaactattt gccgacgctt atcaccacca gcgacgagct
     5461 gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca aaacatccga atcaggcgtt
     5521 aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa
     5581 ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgagaacg ccgacgtgat
     5641 taccaaagtg actctggcac cggaaatggt tccagcggaa gtcatcagca aactggcaaa
     5701 tgccgggatt gtggtttctg ctggtcactc caacgcgacg ttgaaagaag cgaaagccgg
     5761 tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg
     5821 tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat
     5881 tgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaagcgtc tgaaaggcga
     5941 caaattgtgt ctggttaccg atgccaccgc gccagcaggt gccaacattg aacagttcat
     6001 ttttgcgggt aaaacaatat actaccgtaa tggactttgt gtggatgaga acggtacgtt
     6061 aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgtggtat
     6121 cgcactggat gaagtgctgc gtatggcgac gctctatccg gcacgtgcga ttggcgttga
     6181 gaaacgtctc ggcacgctcg ccgcaggtaa agtagccaac ctgactgcat tcacacctga
     6241 ttttaaaatc accaggacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt
     6301 atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc
     6361 gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag
     6421 caaagtcagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg
     6481 ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc
     6541 accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga cgccaccatc
     6601 accttgtttg atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt
     6661 acccagcaga cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac
     6721 cagcgcaagc tacgcgagct gatcgcgatt tcggtgatcc tgccagggct tgtagacccg
     6781 gacagcggca aaattcatta tatgccgcat attcaggtag aaaactgggg gctggttgaa
     6841 gcactggaag agcgttttaa agtgacctgt tttgtcggcc acgatatccg cagtctggcg
     6901 ctggcagagc actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc
     6961 catcgcggaa ccggggccgg gattatctct aacgggcgaa tttttatcgg gcgcaacggc
     7021 aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc
     7081 aactttggct gcctggaaac tatcgccgcc aatgctgcca ttgaacaacg ggtgttgaat
     7141 ctgttaaagc agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc
     7201 tgcaaagccg cgaacaaagg cgatagcctg gcctcggaag tgattgaata tgtcggtcgt
     7261 catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt
     7321 gctggagaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat
     7381 acccaggcgc tgaaggcgtt tcgtactaat ctaccggtgg tacgttctga gttggatcac
     7441 cgctcagcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc
     7501 cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg
     7561 ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata
     7621 acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc
     7681 tggtgttgct gaccaactat ccttcgcaga ccgggcaaga tctggcgaat cgctttgcca
     7741 ctgccggtgt cgatgtaccg gatagcgtgt tttatacctc cgcgatggcg accgccgatt
     7801 tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg
     7861 aactgtacaa agccggtttc actattaccg atgtgaaccc tgattttgtg attgttggcg
     7921 aaacgcgttc ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggcg
     7981 cacgctttat cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcctgtggcg
     8041 cgttgtgtgc agggatcgag aaaatctccg ggcgcaaacc gttctatgtt ggaaagccga
     8101 gtccgtggat catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacggtga
     8161 ttgtcggcga taacttgcgt accgatattc tggcgggctt ccaggccggt ctggagacga
     8221 ttctggtgct ttctggtgtt tcgtcactcg acgatatcga cagtatgccg ttccgcccaa
     8281 gttggattta cccatcggtc gctgaaatcg acgttatctg aatagcaacc acgcctcagg
     8341 gcgtggtttc ttatttttac ccatcattaa aatcacccca ttcaataaaa cccgtcaaaa
     8401 attatcgatc catcaataaa tatacccaaa agccctcccc tttttcatca atcagcaatc
     8461 gccattacgt tttttatttt tccactgcca aatcgcttgc gcaccgtgct gctttgcggc
     8521 atttttttaa caagtaaaca caacacgcag taaacaccag gttaacggag aaggttatgt
     8581 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
     8641 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
     8701 ataacgccat cctcgcccac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
     8761 ctctctacaa ccaacaaaaa actcatgtgc tggcagtaaa cggtgaaatc tacaaccacc
     8821 aggcattgcg cgctgaatat ggcgatcgtt accagttcca gaccgggtct gactgtgaag
     8881 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
     8941 ttgcctttgc cctgtacgac agcgaaaaag atgcatatct gattggtcgc gaccatctgg
     9001 ggatcatccc actgtatatg ggctatgacg aacacggtca gctgtatgtg gcctcagaaa
     9061 tgaaagccct cgtgccagtt tgccgcacga ttaaagagtt tccggcgggg agctatttgt
     9121 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tacgatgcgg
     9181 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
     9241 gccatctgat gtctgatgtg ccttacggtg tgctgctttc tggtggtctg gattcctcaa
     9301 ttatttccgc tatcaccaag aaatacgccg cccgtcgcgt ggaagatcag gaacgctctg
     9361 aagcctggtg gccgcagttg cactcctttg ctgtaggtct gccgggctca ccggatctga
     9421 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
     9481 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
     9541 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
     9601 ttaaaatggt gctgtccggt gaaggttctg acgaagtgtt tggcggttat ctatacttcc
     9661 acaaagcgcc gaacgctaaa gagttgcatg aagagacggt gcgtaaactg ctggccctgc
     9721 atatgtatga ctgcgcgcgt gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
     9781 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaaccca caggataaaa
     9841 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgaa gcgtatctgc
     9901 ctgcaagcgt ggcctggcgg caaaaagagc agttctccga tggcgtcggt tacagttgga
     9961 tcgacaccct gaaagaagtg gcagcgcagc aggtgtctga tcaacagctg gaaactgccc
    10021 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgggagatct
    10081 ttgaagaatt gttcccgctt ccgagcgccg ctgagtgcgt gccgggcggt ccttccgtcg
    10141 cttgttcttc cgctaaagcg attgagtggg atgaagcgtt caagaaaatg gacgatccgt
    10201 ctggtcgcgc ggttggcgtt caccagtcgg catataaata agacgtgtaa tattgccccg
    10261 gagaaatccg gggcaatttc agttactggg gcgcttgttg attctgatat gcagtaagga
    10321 aatgttccgg gtttgccaca tcgctgtttg acaactcatt actgtaggcc agtatttcat
    10381 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtatga caaaagaatg
    10441 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccatgctg
    10501 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    10561 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaaga
    10621 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
    10681 atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac gcaccagatt
    10741 taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga
    10801 aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt
    10861 ccctggttcg aatccaggta ccccagccat cg
//
LOCUS       DAZGTJ010000132        10753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000132 DAZGTJ010000000
VERSION     DAZGTJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.132"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1064
                     /locus_tag="ACHK8E_004564"
     CDS             <1..1064
                     /locus_tag="ACHK8E_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0346733.1"
                     /translation="DMGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGP
                     RGEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETK
                     AATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSE
                     FVAWLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRV
                     TIGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRL
                     RQWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSV
                     TLITVRGCYGKFSWEAIGY"
     gene            1074..1298
                     /locus_tag="ACHK8E_004565"
     CDS             1074..1298
                     /locus_tag="ACHK8E_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0346734.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            1298..1969
                     /locus_tag="ACHK8E_004566"
     CDS             1298..1969
                     /locus_tag="ACHK8E_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0346735.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            2507..4132
                     /locus_tag="ACHK8E_004567"
     CDS             2507..4132
                     /locus_tag="ACHK8E_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346736.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            4129..5397
                     /locus_tag="ACHK8E_004568"
     CDS             4129..5397
                     /locus_tag="ACHK8E_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0346737.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            5412..5690
                     /locus_tag="ACHK8E_004569"
     CDS             5412..5690
                     /locus_tag="ACHK8E_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346738.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            5696..6313
                     /locus_tag="ACHK8E_004570"
     CDS             5696..6313
                     /locus_tag="ACHK8E_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346739.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            6404..7138
                     /locus_tag="ACHK8E_004571"
     CDS             6404..7138
                     /locus_tag="ACHK8E_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0346740.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFINRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGIGVAKVTADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            7371..7511
                     /locus_tag="ACHK8E_004572"
     CDS             7371..7511
                     /locus_tag="ACHK8E_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0346741.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            7568..7969
                     /locus_tag="ACHK8E_004573"
     CDS             7568..7969
                     /locus_tag="ACHK8E_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346742.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            8063..8719
                     /locus_tag="ACHK8E_004574"
     CDS             8063..8719
                     /locus_tag="ACHK8E_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346743.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            8722..9168
                     /locus_tag="ACHK8E_004575"
     CDS             8722..9168
                     /locus_tag="ACHK8E_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346744.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAV"
     gene            9178..9429
                     /locus_tag="ACHK8E_004576"
     CDS             9178..9429
                     /locus_tag="ACHK8E_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP0346745.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            9440..10702
                     /locus_tag="ACHK8E_004577"
     CDS             9440..10702
                     /locus_tag="ACHK8E_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346746.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
BASE COUNT         2809 a         2259 c         3153 g         2532 t
ORIGIN      
        1 gggatatggg gccaaaaggc gagaaaggtg atccgggagg tcctgcaggc ccgcaaggtc
       61 ctaaaggtga acgaggagaa gccggaccac agggaccgat gggagcacga ggtgagcgtg
      121 gggagactgg cccccgaggt gaacctggtc ctgcaggtcc gagaggcgaa cgaggagaga
      181 ccggacctca gggacctcgt ggagagccag gtccggcagg cagcgctgca aatgtggctg
      241 atgcgacgac ggcacagaag ggaattgtgc agttaagcag cgcaacggac agtgatgatg
      301 aaacgaaggc agccaccccg aaagcggtga aagcggcaat ggacaaagcg gacggatgcc
      361 tggagaaagc gaaaaacggt gacgatatcc cggataaggt gaagtttctg aacaccgtgg
      421 gggcagccag agtatacggg cgggacattc atacgggggc cggtgaatgg accacgagcg
      481 agtttgtggc ctggctgaaa gaaaaggggg catttgacca gccttactgg atgatgaagg
      541 catcactgct tgcggaattt aataaggtca tcacggatgt cggaccggga aaactcaatc
      601 tgggaggctg cgccattgag gtgatgggga cgtataacgc agccatagtc cgggtcacca
      661 tcggcgaata cggtggcgat ggttttctga acggcacggt ctgtacctgt acagtttacg
      721 gagacacaca acgttttcac tggcgggtgg attacagcac aaaaaacaag cccacaacag
      781 caagcctgac agtaaatggc tgggagcgtg atgaagtaac cggtcgtttg cggcaatggg
      841 gcagtattga ggtaagtgag gatgatggta agcttatgac acagcactct atccgttttc
      901 ctgtcgcttt tccggttgct gctctgaatg ctcaggtgtc tgcagtggga gacccccacg
      961 ccattaagat gtataccctg tcgaatccgt cattagtgtc tgtaactctg ataacagtca
     1021 ggggatgcta cggcaagttt tcatgggaag cgatcggata ctgagggcat tgtatggaac
     1081 agtatgtttt ttcaccgtca gagaatatgt tctatcccct gtccctgcgg ccggtttatg
     1141 aggcagcagg gcgctggccg gaagacggta ttgtggtgga ttatgtggtg tataaagtct
     1201 ttgcagcgga tgctgcaccg gcaggtatga agcggggcgt cggtgcggaa aaaatgccgg
     1261 tctgggtgcc ggtatcggag gagggcacgg aaacatgatg catatcagaa atttttcgta
     1321 ttacaccccg gcggagccgg atgttgcagg cgcgatgtac ctgaagtctg aggatggtca
     1381 ggactggtat gagtgtcagg cgttgttttc gccggagacg ctgaaggtgg tgtatgacag
     1441 ccggggtgtg attacgggat acggtaagga cactgcgtta ctgtggccgg tgaaccagag
     1501 tgtggcagaa gtgccggata ccccggagag ccggaaaatt gacctgagcg ggcgctgggg
     1561 ttttgacggg gagaaaatca cggacctgct gaccgcggag aaagcgcgag ggatgaaggg
     1621 cgatgaaata aacgcgtggc gtaatgcgat ggaagcggcg aactacacgt ttgagcacaa
     1681 tgggcgtaaa tgggactacg ggaagtcaac gcagacgcgt cttgagccgt cggtggcggc
     1741 agcgaaatcg gggaaactgc cggaggcgtt tttctggacg gatgcggaaa acaatgatgt
     1801 ggaggtgaca gcagaagagc ttatagcgct gagtgaagcg gcagagcagg cgatgttcac
     1861 caaagggatg gaaatccacg ttcgccagcg caccatgaag aaagagctgg aaaagctgac
     1921 cagtgcggat gagatactgg catacagggt tggctgggca caggaataac agacaaaaag
     1981 atgggggacc atcacctccc ccatgaactg agtcaattat acacttgtga agtatttact
     2041 atgcgaataa atactgtcac ggaaagtgca ggcctggatg tgatggaaat catgtttccg
     2101 gcgcatcctg ttgatatgga taatttttca gatttccgga agtgagagtt tttggtgttt
     2161 ccataccagc cacagttgat gtgtgttgtg gcacctggca gtacaattaa gggaacaggg
     2221 gtaataaaaa acgggggcat gaccaacccc cggtacaagg aaggagtaac tacaatggaa
     2281 aaatcaatga agacattcag tgctatgtta tatggcgaat aaagactgcc acagggaagt
     2341 ttgcagaaac agtgatgccg atcgtgtttc tgacgctctt ctctgataag agtaattttt
     2401 cagatttctg gaaatctgta ttctgcacag gcgcaattgt atgccgcctt taaaacttca
     2461 attcagtgac tcacctgcca ttcaaatttt cggataccag acaaccatgc cttatatcga
     2521 tataacaact atgcgcggga tgatgccagg cgttattgca tctatgctgc cagatcattc
     2581 tgctgtactg gcagaaaact gtcattttcg ctatggagtg atcacgcctg aacaccagat
     2641 gtcagaggct gagaaaacat ttgcgattaa gccgaaaacc atttttcatt accgtgacga
     2701 tttctggttt gcatggacgg atgtggtgga tgtgatccgc agtccggtcg ctcaggacgc
     2761 ccacgggcgt atttactaca ctgacgggcg ttttcctaaa gtgacggatg cgaccattgc
     2821 cacaaaaggg gacgggaatc acccgacatc atcgtatcgt ctggggattc ccgcgccgac
     2881 aacagcacct gtctgtactg ttcagcaggg cggtgatgtt tctgacgata acccgaatga
     2941 tgacgaaacc cggttttata cggaaacctt tgtctcagat tatggtgaag aaggtccgcc
     3001 aggtccggcg tctctggagg taacactccg tactccgggg actgcggtac agctgacgct
     3061 gtctccggtg ccattgcaga atgccagtat taaacgccgc cggatttatc gctctgcatc
     3121 aggtggagga gaagcggatt ttttacttgt ggctgaactg gatgcatccg tgctcagtta
     3181 cacggacaaa ataccgggga aaaaccttgg accttctctg gctacatggg attacctgcc
     3241 gccgccagag aatatgacag gcctttgcct gatggctaat ggtattgccg ccgggtttgc
     3301 cggtaatgaa gtgatgtttt cggaagcgta tctgccgtat gcatggccgg aagtgaatcg
     3361 tcacacgacg gcagaagata ttgtggctat ctgtccgctg ggaacgtcac tggtggtggc
     3421 gacaaagggg gagccttatc tgttcagtgg tgtatcgcct tccacaattt ctggctccag
     3481 aattccttcc atgcaggcat gcctgagccg aagaagtatg gtggcgatgg agggattcgt
     3541 actctatgcc gggacaaacg gtctggtatc tgttgatgta aacggtaata cagcactggc
     3601 aacggaaaag attatttcac ctgaacagtg gcagagtcag tttaacccgg cgtccattgt
     3661 ggcttattcc tggcgtggtg aatacattgc ctgttacacg aaaccggatg gtaagcagga
     3721 tgtgtttgta ttcagtccgg tgaacatgga tatccgttat ctcagtacac cgtttgactg
     3781 cgcatgggtt gatctcgcga aagatatgat gcgtgtggtg acaggagaca aaatgtcagt
     3841 gcttgccggg ggctctctgc cctccacgat aaggtggcat tcaaaaattt tttcattacc
     3901 tgaaagaacc tctttttcct gtatcagggt gaaatctccg gcgcctgagc gggtggggat
     3961 caccattatg gctgatgatg ttcctgtgat tcattttgcg ccgggtacgt ttaagggaag
     4021 tgtggtgaga cttccggcag caaccgggca aaactggcag gtgatggtat ccggattcgg
     4081 gcaggtggaa cgaataaccc tgagtacatc gatgtcggag atgccggtat gaccagaaaa
     4141 ccgtggcgtg cggggaagga tttatccaca gttgtggaga acatggaaat tggcaccggg
     4201 cagcgtggtg acggacgcca cgcatttgtg acccgtgagg aactggttgg tcttaaactc
     4261 gcccggcgtc gaacatcggg tggtgcctca tatgcactga atccgggtat tgagattgac
     4321 agtactttaa tgactgttga ttttcccaca aaaccgctga attttaaggc gacaggagga
     4381 tttggctcgg ttcttcttga atgggatatg cctaattatc gcggacattc actgactgaa
     4441 atctggcggg gtacggagga tgaccttgct gatgcagtgc tggttgccac gacgccgggg
     4501 caggtttacg gcgatccggt tgaccctggc tggtcgggat tttactggat acgttttgtt
     4561 aacgcggcag gagtgaaagg tccatggaat gctgaaaaag gcactcaggc acaaacacag
     4621 atcggcgtga aggccatcat tgaccagatc cgcgatgagg ctgcaaagtc gccggttgtg
     4681 tccgagctgc gtaaagaaat aaaaaacgcg caggggcagg ctgtaaagga tgctgcaatt
     4741 aagacaaccg aagttgtggg gactctcagg gaagaaacga caagaacgat tggtggtatt
     4801 gaaacccgca ttagcacact ggattcgtca accagtgaat cgcttaatga ggtcgacaag
     4861 cgcatcacta aactggataa agaaggcggt gaggcgtttc tggcaatgtg gtcaaaaaaa
     4921 gcgggagttg atggtatcac tgcggggatc gggattgtcg ccggaaaaga cagtgaaggc
     4981 aggcctgtaa gtcaggttgc aatttctgcg tcgcagttgt ttgtctttga cccgaacaac
     5041 ccggataaca ccgcctatcc gtttgcggta tcaggtggca aggttgtgat cccgaaagcg
     5101 atgatttatg acgcggtgat tgaaacactg gtgtcgcgga aggttgtggc ggatgaggta
     5161 aaagccgggg taagtatcac ttcgccagtt atccggagtg ccgttattca gaacggaaac
     5221 tttcaggttg attctcaggg taacctgaat attggaggcc ttttcagtgt tacgtcacaa
     5281 gggcaactga caattcgtta ctctaatcag aatgtaggac tggtgatccg caatgataaa
     5341 attgaggttt atgatcagaa tggacgactg gctgttcgca taggcagatt acgctgatca
     5401 ggaggtgagt attggaatac ggttttgcca tttataacag aaataacgtt aatgttacgg
     5461 gcgtgctgac tccggtattt ttcctggaca gatttacagc ggagtctggc tcaaagacgt
     5521 acacgaataa acccgacggg aaatcattgc aggctgtatg ttgtttattt ccctggaata
     5581 atgtatttgc ggatcggaaa gtaccgaaga taaccattaa tggcaatacg gtgacgtggt
     5641 cgaatcttga gcagggtatg ggatcttata tttatacatt ctggggataa gtgtcatgta
     5701 tggtttgagc attatgaagc cggatggcag cgtatggata agtccaggtt ttacgccgca
     5761 gtgtctgatc aacaaaggca ccataccggc gactgaaaag tcttttttta aaacatcaat
     5821 cccgtcaggc aaaagttgtt ttttctttat cagaacagag aagaaggccg atgtcatgta
     5881 cacgcatgaa cagattgatg gatatcatgc actaaggctt catgtaattg tcaggggaac
     5941 gaaccctggt gttacgacgg tttatgcttt cgcgaatatg gttactccac cttctgagta
     6001 tggtatcgcc atgtataacc cggacggtga gatgatttat catggcgaaa tgatgctgct
     6061 tgacgcgaag ttaatacctg ttgatatcaa atttgaaaag gaccttggat atccatgcgc
     6121 aatcatgcct gcactggtcg ggtattataa ctggaaaaga actccttatg atcgaccgat
     6181 ttataccaca tccactggtg ctacaggaaa taaaatatat tcctgtgagc attattccgg
     6241 tggtgcaaca tgggatattc gaaagccgta tatagataag gtcctggtta ttaatacatc
     6301 agtatatgat tagttgaagc gagtctttaa tattcattta aaatgtctaa aaagatgtat
     6361 tattaaaaag tttagcgtgt tatctgaata caggatatct taaatgaaga gtatagcaac
     6421 actggttgtg tgtgcaatct ccgggattgc ctgtgtaaat ttatctgcac atgcagcaga
     6481 aggagagcat acaatttctc tggggtatgc gcactttcag tttccgggac tgaaggattt
     6541 tgtaaaggat gcgactgctc ataacaggga gactttcagt catttcatca acagaaacta
     6601 cttttcttca ttgggcgaat atacagatgg tcgggtcagt ggatatgaag gcaaggataa
     6661 aaatccacag ggcattaata tcaggtatcg ctacgagata acggatgatt ttggcgttat
     6721 cacctctttt acatggacgc gttctctcac taactcacag acatttattg atgtgcagtc
     6781 agccgatcat accaggaaga ttaagaatcc ggcagcttct gccagaacgg atatcagggc
     6841 gaattactgg agtctgttag cggggccttc atggcgggtt aatcagtaca tgagtttata
     6901 tgcgatggca gggataggcg tggctaaagt taccgctgac ctgaaaatta aggacaatat
     6961 taacagtagt ggcggatttt ctgaaagcaa cagcacgaaa aaaacctccc ttgcgtgggc
     7021 tgcaggtgca cagtttaacc tgaatgagag tgttacactg gatgtggctt acgaaggttc
     7081 cggctctggc gactggcgca cgagtggcgt tactgctggc attggcctga aattctgacc
     7141 tgtatccggt aaccatttac tacccgctgt gatggcgggt tttttattgc ccgtacaggg
     7201 caaaaatcgt aaattatgcg tgggtgcctt tcggctgatg gctggagggt gaacctgaag
     7261 gcctgatgtg gaaaggcccc gagtcaactt aacgttaacc cgaggcccta acacttcgta
     7321 ccttaagcaa gtagaaggtt agcgcctctc tgtaaaagga gtcaagcgct atgtcgcaaa
     7381 aatcgcttat caccgtcaca atttgtatga cggttatctt caccatctgg atgttacacg
     7441 gctcgttgtg tgagttccgg ctgaatttgt ggggagcgga gtttgcggcg ttcttacagt
     7501 gtaagcagta ggaaaaccgc ggcggggacg aaagtccccg tcaactggtt gctgaggttc
     7561 agccgatatg gcacccgttt caggtgagag aatgaacgat aaaattctcc ggtatatgca
     7621 gcgtgtggtg agaaattccc gcaaccctga atttatgaat gaagttaaag acgcctgcct
     7681 taaaaagcag gcgttttgtt ttgaggctcc tgatggcttt ctggtgctgc gttctgtgct
     7741 cagtgacgat ggtatccctt atgttctggt gttgctgggc gtgtgtacgg ggagtaacag
     7801 cgttgagcgt tatctgccgg aggtgaagac attaacccgt ctggctggcg gacgctgggc
     7861 tgaatttcat acggcaaggc ggggatttat ccggctggga aaacgtctgg gctttgagcg
     7921 aatgccggat gatgaggatg gcttcatggt gttcaggata gcggtctgac tgctacagtt
     7981 ttcatcattg tgtttaaacc aacattgtaa ttcacattct gaccctgctc cggcagggtt
     8041 ttttgttatc cagggggcca ttatgggtgg aagtaaaggc ggtggtgata ccaaagtaaa
     8101 accaacagca gcgcaaatag cacaggaaga agtggcctgg aaagggtggc aggattatca
     8161 aaaaatcctc cgcccggctg aagataactt catggaaaag gtcgatgatc tgaacagtga
     8221 gcagcagtac gacaatattg ctggcaccac aaatctgggg tatcagaaac agtttggcga
     8281 agcacggaag gagctggcgg gtaatcttgc tcagtccggt gttgacccgt ccagtggtcg
     8341 ttttaacgcg gtaatgaatg cgaaccagag tgatcaggta accgggcaga ttgacacaac
     8401 cacacggggg caggtatcgc aggcagataa gtatgttgcc gggctacagg atgttgcagc
     8461 tctcggttct ggtcagaagg cggatgcgtt acagagtttt aactcgctgg cagacagcag
     8521 tctggcaaaa gctaaatcgg acgcacaggc tgcgtttacg aaacagcagg ggcgagcctc
     8581 tcttgttggc gctggtttgg gtgcggcagg tgcatatgcg atgcataagg ctggcggtag
     8641 cggaggaagt ggcggtgcta aaacacctgg caccggcgct aatgccattc agcatcaggc
     8701 tcagaactgg agactgtgat tatggagtac ggtaaatacg aaacacttgc gagatacggt
     8761 tataccggag ctgcccgtcc acagggtgac tggcagacgt cagcagcgct gacacgccaa
     8821 caatacgacg actggcgaac cagatatttg ccccgcgtgg caaggctggc tgaccttggc
     8881 gagaacaaca gcctgatgaa tgcacagctt gcccgggtgg gcggccttgc cacttccagt
     8941 ctccgtacag cgcagatggc gcaggataac cagatggcga gatacggggt aagccgcccg
     9001 gataatcccg acagtaatac gctggggtta cgtaatgccc tggcaattgc tggcgcgaaa
     9061 aatggtatcc gtgaagccga acaggatcgc cagatgaata ttctgacggg ggcttctgca
     9121 ccggcaagac agaaactgag tgttggcgga caactggtgg cagtgtaagg gggcaatatg
     9181 ggatacggtt tactggatat tgcaaatcag tcgcggcgtg aggcattaca gggaataagt
     9241 gacgcagaca gacgacgcac agagattgag gcatcaaata aacaactggc tgctcaacag
     9301 aaagctcaga aaaaacagaa tatcggtacg ggcattggta cgggggcggc tattggcgca
     9361 tccgttggtg gtcctgttgg cgctgttgct ggtgcagtaa ttggcggcat tgctggttct
     9421 ttgttttaag gagtggtgaa tgagcggatt tgcacagggg ttacttgccg gattcagcac
     9481 tgttgaccag gcaatgaccc gtcgtaagga gcttggtttg cgagaagcac agcttgctca
     9541 gcaacagaaa aataacgagc gcgattttga atttgcgcag tctcagtttg aacataataa
     9601 agacgttgat cagcggaact ttgattacag agccaaagtt gatgaccgca attacacact
     9661 gcaggaaagg gagtttaacg ccaaccagaa ttaccggaat gcgtcgctgg ggatggaaca
     9721 gcaacgactc cagttgcaga aatacaacca gcgacggctt gagtataacg atatgctggc
     9781 gcgcgatcag cctgtgatgg cggcgctggg aaaagcgatt gatgcgggtg accatgatgc
     9841 ggcttctcat ctgttcggta aattatcgga cgccaatccg cttaagatga tgtcaacggt
     9901 aggctatgct gcgaaagcgg gtcaggccgt gaacaacctg cagaaaatct ttgatgataa
     9961 gccggacagg gcgattgctt cgctgaatac cccggaaaat ctcgatgtgc tttccggcgt
    10021 gtttgccccg gaactacaac agcgtattgg catgcctgat tcaaccgggg agaaaacgat
    10081 aaaagaggcc aggattggca gtatcgtacc ggcgcagcag gaagggtacg tacttattgg
    10141 ccttgatctc acatacagcg atggctccac cgcgcataaa cctgtaacag aatacggcag
    10201 tgcgcaccct gatgatcaaa ccgtgctggc gatacccgtt gataaggcta tcgctcaggt
    10261 cagggatcgc agcaaatttg cagagatatc gaaaaattat ggttatttta tgccgaagca
    10321 gcagggactt tctctggcga aacttcagga agtagccagc aacgtagcgg cggatgcgat
    10381 caagaatggc cgtgatgctc aggctgaggt ggatgaattt tatgctgcga atggtttaca
    10441 gccgcatcaa cagaaagttc agcaacggaa aacgcaacag cttttacaga agtggcagac
    10501 gtgggcggcg ggcgatccgg ttaaacagga gtttgtaaaa gaggctgccg ccaatgcgcc
    10561 tgctttgctg gaacctggtc gtgaaaagga ggtggaaatt tattaccaga atcacctccg
    10621 ggataaaaag gcagagcatg aaaaggcgct ggattatagc gcttccgcag ctgccgagaa
    10681 gatcaaaggc tggatgaagt aaagatatgc ggcagtaacg cgctccccgt taatatcgtt
    10741 cacttacgcc cga
//
LOCUS       DAZGTJ010000133        10740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000133 DAZGTJ010000000
VERSION     DAZGTJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.133"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHK8E_004578"
     CDS             complement(<1..209)
                     /locus_tag="ACHK8E_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346747.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGG"
     gene            complement(289..762)
                     /locus_tag="ACHK8E_004579"
     CDS             complement(289..762)
                     /locus_tag="ACHK8E_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346748.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAFCACIAWIPFIIA
                     AFGVDGIYLKILFAVLACCGAVCFFICFFTLLPESLMVLSRQSLVQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQENVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(755..1168)
                     /locus_tag="ACHK8E_004580"
     CDS             complement(755..1168)
                     /locus_tag="ACHK8E_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346749.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(1161..1382)
                     /gene="traR"
                     /locus_tag="ACHK8E_004581"
     CDS             complement(1161..1382)
                     /gene="traR"
                     /locus_tag="ACHK8E_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFP0346750.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(1517..2032)
                     /gene="traV"
                     /locus_tag="ACHK8E_004582"
     CDS             complement(1517..2032)
                     /gene="traV"
                     /locus_tag="ACHK8E_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP0346751.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(2029..2280)
                     /gene="trbG"
                     /locus_tag="ACHK8E_004583"
     CDS             complement(2029..2280)
                     /gene="trbG"
                     /locus_tag="ACHK8E_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFP0346752.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            complement(2292..2486)
                     /locus_tag="ACHK8E_004584"
     CDS             complement(2292..2486)
                     /locus_tag="ACHK8E_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFP0346753.1"
                     /translation="MNMRNIHVITARSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            complement(2473..3057)
                     /gene="traP"
                     /locus_tag="ACHK8E_004585"
     CDS             complement(2473..3057)
                     /gene="traP"
                     /locus_tag="ACHK8E_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP0346754.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            complement(3047..4477)
                     /gene="traB"
                     /locus_tag="ACHK8E_004586"
     CDS             complement(3047..4477)
                     /gene="traB"
                     /locus_tag="ACHK8E_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP0346755.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTFSYDAGTKQTPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            complement(4477..5205)
                     /gene="traK"
                     /locus_tag="ACHK8E_004587"
     CDS             complement(4477..5205)
                     /gene="traK"
                     /locus_tag="ACHK8E_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFP0346756.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGKLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLAAARMDVYVIRDGEGN"
     gene            complement(5192..5758)
                     /gene="traE"
                     /locus_tag="ACHK8E_004588"
     CDS             complement(5192..5758)
                     /gene="traE"
                     /locus_tag="ACHK8E_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP0346757.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(5780..6091)
                     /gene="traL"
                     /locus_tag="ACHK8E_004589"
     CDS             complement(5780..6091)
                     /gene="traL"
                     /locus_tag="ACHK8E_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP0346758.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(6106..6459)
                     /gene="traA"
                     /locus_tag="ACHK8E_004590"
     CDS             complement(6106..6459)
                     /gene="traA"
                     /locus_tag="ACHK8E_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP0346759.1"
                     /translation="MNTSVSVPGAFAPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            complement(6494..6721)
                     /gene="traY"
                     /locus_tag="ACHK8E_004591"
     CDS             complement(6494..6721)
                     /gene="traY"
                     /locus_tag="ACHK8E_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFP0346760.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
     gene            complement(6840..7487)
                     /locus_tag="ACHK8E_004592"
     CDS             complement(6840..7487)
                     /locus_tag="ACHK8E_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFP0346761.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFIWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQRM
                     DVSIDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINNLHCKET"
     gene            complement(7681..8064)
                     /gene="traM"
                     /locus_tag="ACHK8E_004593"
     CDS             complement(7681..8064)
                     /gene="traM"
                     /locus_tag="ACHK8E_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP0346762.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            8397..8987
                     /locus_tag="ACHK8E_004594"
     CDS             8397..8987
                     /locus_tag="ACHK8E_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0346763.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLLFINVTC
                     EAGDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQQRLA
                     YASDVYRIYSGIKNSKGLQLRASEKPLSKMNSSQKN"
     gene            complement(9283..10104)
                     /locus_tag="ACHK8E_004595"
     CDS             complement(9283..10104)
                     /locus_tag="ACHK8E_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0346764.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREEHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEKFRELKS"
     gene            complement(10215..10511)
                     /locus_tag="ACHK8E_004596"
     CDS             complement(10215..10511)
                     /locus_tag="ACHK8E_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346765.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNNAVKASLAYQSNQDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(10538..10738)
                     /locus_tag="ACHK8E_004597"
     CDS             complement(10538..10738)
                     /locus_tag="ACHK8E_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0346766.1"
                     /translation="MPQNFLPKSRQKLLRSAPGQSFPAVAVMPHAAKVLKQVMVNTGG
                     NKEQVPAKRQNRGSRNSTDDGG"
BASE COUNT         2859 a         2750 c         2257 g         2874 t
ORIGIN      
        1 ccaccattca gtgtttccat cacgttattc aaaggttcat tagttttaac ttcaatacac
       61 tcccctgtat ttccaaatcc cctgcctcca tgtgcttcga ttgtccgaag aacctgcacg
      121 catacgtccg tatttggcat gggggtaaca ttccagcttc cgccaccgtt aatatttttt
      181 atcagcacaa gaaaaataaa ggtatgcatt ctttattacc tccgcttgta tattaatcac
      241 tgaatatccc ttcagttaac ttatgaggtc ataaaacact gcagattctc attcggattt
      301 attcccttca gtaaacttcc ggatggtatc ctgttcacgc tgacgggtgg cagactcctg
      361 cattgcatcc acgttctcct gccagagacg ccggatatcc ttttcatccc gaccagtcag
      421 ttttttaccg gacagcagac ggttaagcag ttcctgtctc gcatccggta cgtcttcagt
      481 cagacgcata agctgaacca gactctgacg gctgaggacc atgagtgatt ccggcaggag
      541 ggtgaagaag caaataaaaa agcataccgc accacagcag gcaaggaccg caaacaggat
      601 tttcagatat ataccatcaa cgccgaacgc agcaataata aagggaatcc atgcaataca
      661 cgcacaaaac gccaggatgt tatctattcg gttatgtctg cgatatatct gttgatgttc
      721 atccagaata gaatctattt tttcagcttc tgatttatgc atagtgtttc ctctgcgttt
      781 cctgccagag acgccggata tcctgttcat cccgaccagt cagtttttta ccggacaaca
      841 aacggttaag cagttcctgt ctcacatctg gtacgtcttc agtcagacgc ataagccgga
      901 gtaattcctg acgactgagt accatgcgtg attcctccgg tacgctgtaa taattaaaaa
      961 tacgactgaa taccccacaa cagcaggcaa cggacaggca caaggatttc agatacaggc
     1021 cagaaacaca gaatgtggca acaccacaaa gaatagctat aactaaaaag cccttaccca
     1081 gtgccataca caaagaaccc tgttttctga atatccgctc atgctcatcc agaataaaat
     1141 ctattttttc attttctgat ttatgcatag tgtttcctct gcctttcctg atacgcctga
     1201 cattcaacgc acaacgtcac accgggaaat attttccgcc gggcttccgg aataggattt
     1261 ccgcatgctt cacagagata aacaggaata ccatgaacat ttattttctg gcgtatccgg
     1321 tttattcctg tcatggtcag ttgttctgtt actgaatatg cttcatcggc ttcatcactc
     1381 acggaatatt cctcccgaca tgttctgaca cgctaccgcc catccctttt cacgcacagg
     1441 gcactcgttt tacaggcaga ttttgaatac agaaagcccg ccggcattgc cggctgatat
     1501 acaacttcag ggccagttaa ttaatacgtg gttttcccca cgcagacggt ttaataacaa
     1561 aaaacacact ggatggctga tgataaacat cctgattatc aatataaggc gctatccata
     1621 atgctgccgt ctgctcaccc gttcttagcg gacggggagg tattaccggt gcaactgaac
     1681 tgaccggaac aaccgttttc acttcccggg cagcggtaaa cagcggacgt ggtgccagca
     1741 gtttctgctc cggaagtgcc gtcaccgcag gtctgctgcc ggacggagag gtcgcggtga
     1801 cggtctgcac cggcattgtc cggaagttcc cttcagccag gcgctgcagt gatgccgcaa
     1861 ccggctttgc ttctgaggac tgctccagtt ttttggcctt ctcattggcc tgctccatcg
     1921 tcatacaggt atcggatgtg gtggcatcac actcaaagtc ggagtttgtt ccggcacagc
     1981 cggaaagtaa taacgtcccc agcagcggaa taaataaaga aatctgtttc attgtccctc
     2041 acctttctca tcgcagttca caaaatcatt cttcaaatcc ctgaccgtac aggtttttac
     2101 aatgtcagga ttttcaaaaa ccgtattccg tgatgattta taaacatatg ccacaatgcg
     2161 tttcccaccc gcatcaggtt ctggtgcact gacttcacat aacggaggtg ttattccgga
     2221 ctcatacagc accggataat ttattttttt gacgctgcca tcagaaacca gcttattcat
     2281 cgaagggttc cttatatttc tgaattcttc atcacaccga cagcagtcat agtaaaattg
     2341 tccggcaatg actcagaacc gaatttctcc ggtgcaggaa gtactcctgt ggtgcagtta
     2401 ctgagaacag catgcataaa atcatctgat atgcgtttat ccggtacaga gcgggcagta
     2461 ataacatgaa tattacgcat gttcattttt atctccgttg tttctgtttg ttctgttatc
     2521 agggaaataa gcatggcatt tcctgatttt tcccggcagg gaagcgtctt ccgtataaac
     2581 ttccttcaga tgttcatctg cggttaccac ccggatacat ttcatttttc cgggtcgcac
     2641 cagatatttc ccggtgattg ctgccagcga gacatacatc agtgccagtg ttgcccataa
     2701 tgcggtgacc agttgcatca gtgaccggtc agtctccctg acataatctg cagcatcagc
     2761 agcacggtaa cggcagattt tctcttctgc tcctggaaga tctggcagag aaggcaaaca
     2821 ttctcttaca ggaaactgcc cggcaggggc tgtttgtctt atgaagtcaa tttcctgaac
     2881 cagtaatttc cctggtgtgg tgttgtcctt ccataaaaca aataacgcca tcagggccat
     2941 tgtggccagt ggcagaatga cggtgtattt cagacaccag aacagtccac gcagaacccg
     3001 ggcgacaaca taccgggcgg tatggcacgc ctgactggac gacgtattat ttgccatcgt
     3061 tgcccccttc ctgaccaccg gccataaact gctgcggatt caggtcgccc agctgcttca
     3121 gcatctggtc ggtattcaga ccattcaggt gactctctgc ggatggcgga ttcggcacgg
     3181 gacttcccgg gttactctct tccacctgcc cctgagcctg ctccagcgcc atctcttcca
     3241 cggttttcag ctggaaaccg tcctggaaca ccacggtcac ttcattaccc gccccgatgg
     3301 gtatcaccgg gtgatactgc tcagcgcgtt tgatgtaata gtcactgagt gtctgtgccg
     3361 cttttgatgc accgccaccg atacccattt tcaggacatc tccggccccg taagcggcag
     3421 tagctcccag cccgacagct ggctgtgaag ctcgctccat tccctgaccg ataccgtcaa
     3481 caaatcccgc gccccatgcc cagccgagga ttttgccgtt acgcatcacc acttcaccct
     3541 tgataccgtt tttaccccgg aagctgacat gccctttaac cggcatatcg atggttttac
     3601 cctccttcag gcagctgata ttgcgggtac gcacgatggc acgctcactg gacacatccc
     3661 cccaggcttc cagcccaaca aagcatcccg ttgcgtcata ggttttgctg ttcggcattt
     3721 ccaccagacc ggtgatacgc agctgcatcg gcaccgtgga ttcattaccg gtgacagaag
     3781 cattggcatc cgccccttca atcagaatgg ctttcgcaaa actgcctgac ggaatgtacg
     3841 gcagggacgg agtttgcttt gtgccagcat catagctgaa cgttttacgc tgtatccggt
     3901 tcggcaccgg cacagactgg tacgtcacct gtggcggcgg cgtggctccg ttgcccggat
     3961 agaacgcggt gggcggtggc actgcacctg ccccctgcgg cggaaaagca acgggcgcat
     4021 taccgggctg aggctccccc tccggaccgg gaggggatgc cggcatctgt ggcacaggct
     4081 ctccggtcac tgtaacggga ttcgcgccca gggctttcac ctgctctgcc agcgcagcat
     4141 tctcctgccc cagcttttca atgcgacgct ggtcatcacc acgctgtttg ttcagaacat
     4201 ccatctcacg acggatttcc tcatactgct tctgcatctg tgccgccgtc acctgcatct
     4261 ctgtggtggc atgctgacgc accttgtcat caaaggtggt atccacaaca ccggtcatat
     4321 ccggcacagg ctcctgccct gcaacggcat cagtgttccc ggacatatcc atatcagaca
     4381 gataaagcgc accaccaatt gctgataccg atccgacaat cacaatcccc agccacagga
     4441 actgcttgcg tttcacaatg gtgttgatac tggccatcag ttgccctccc cgtcacgaat
     4501 gacgtacaca tccatacggg cagcggccag taactggctg acaggctggc tgaacatcac
     4561 cgcccgggtt cccggctgcc agaaatcact ttcccggata ttcagcgcag acagcgtttt
     4621 attttccacg gtaaagcgca ccatcttcag gtgattcccc gtccatacgg cttcagcttt
     4681 tgcggacatt ccggcagggg cctgcagggt ttcctttgtc acggggatct gataccagcc
     4741 ttccggcagt tttccggcac ggaccgcctg actgatggaa accagcaggg attcgtaagg
     4801 cgtggatgtt tcccatgcac cggcagtttc tcctgttccg cgcaaatcgc tgaccagctg
     4861 aatggtacgc ccggctcctt cacggggaac ggcctgaatg gaaagattaa gaccacgttc
     4921 tgtttccaga atgaacgtaa aaggcttttt gttgacggtg gccaccacca caccaccgga
     4981 tgcagtctgc tcattattgg tcagcgcacc atccagactg ttcacggcga taatccggtc
     5041 accggggacg gtaaacagat tcgggctggt attactgatg gcaatacttg cctgaccgcc
     5101 attgaccatc ggaaccactg taggtgccag tgtgccgttt cctgccagca cgctgccgga
     5161 aaaaagcagg caggcggata ttattactgt cttatttttt ctcatcgtct gtttccccga
     5221 aattatccag ccaggtcacc ccgttttccc gtttcagaat aagaatgtaa tgtttgatat
     5281 cagtgaaagg ttcggagtta ccaatccatg ttttccgaat cccacggatt tccacacgac
     5341 catattgtgg ccagacacga acactggtct ggaaaaaagc agaattcaca ttatcttttt
     5401 tgatgagctt tgcttcttcg gccagaatca ctttcatctt attctgcgcg cccggacgga
     5461 tatactgcag aagagcctgg tgagaagcat cgacgatttc cggtgagaca ttcaggcgca
     5521 gggcaataaa cgccagtgcc atctgctgca aaaaagaggc atcagcactg ttctgtgata
     5581 ccgcaaaggg catattaaat cccatcggtg tcacggcagt acgctgttca ttctgaaggc
     5641 ggtaattatt caccccctga atcacattaa cagtcaggct gagaacaatg agcagggaca
     5701 taaatataaa agcaatggcc agtacacgac tggtacttaa acgggcaccg tgttccatat
     5761 aacaatccag gtatcagttc tatttaatcc actgccggaa acacgaatca ggaacattat
     5821 gaaaaatacc gcgcagcagg actgttggca tataccagta aataagatcc cgtaaccagg
     5881 agctgccccg cccttttttc agttttttaa tcccgaaata aaccagaacc gccgcaccaa
     5941 taccgaacag atatttcgat gttgtgatac cccagccaat acagatcgct gcggggatca
     6001 gttcatccag tggcaggcca aaccagcggc tctggttggt cagtgtttcc gggaaacgat
     6061 atttcttaag tttattctcg tctcccgaca tcgttttatt tcctgtcaga ggccaacgac
     6121 ggtcatacct acagcaataa atacagatat gatggcaaaa ccggccagga acttgacgtt
     6181 tttggtcatc atgtacatga ccgccccgac cagcacttca gccagaacga cccatttaac
     6241 aacactggag tccttaccaa aggtggcttt gacggtgtca ttcccggact gcatgaggtc
     6301 ctgaccgcca gcagccaggg ccatctgagg gaagaacatc atcagcacag cagccgggat
     6361 cacagcacga accaggcgaa gcaggttcag tttagagaaa aaagatgatt tctttttaac
     6421 aggcgcaaaa gcccccggaa cactaacaga agtattcatc aattacctcg gattgtatgt
     6481 aaaaagatta aaactaaagt tctttaaaag ttgattctct ctcttctgtc acctccctga
     6541 atatttcttc gttataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg
     6601 cttcatttgt tttcgttctt ccactgcgct ctctggctcc aagtaataat ttattggttg
     6661 cctcatcaag ggtcagtaaa acctttctac ttggagcagc tcttaaagca cctttgatca
     6721 ccacacctcc cgtacataaa agagatttag tcaaaatatt cacataaagc gcacacaaaa
     6781 caaacaacaa aatagtaaat tacgttaaca aaaaccggca cttttagtgc cgggaatact
     6841 tacgtttctt tacagtgaag gttgttaata ttactagcga agtattgata tagataaaat
     6901 aacacatcag aaattatagc atatcgcaac cactcatccc tgtctgagaa accatagtgt
     6961 tttttaaaat tcataaatat ttttttagac tggtcaatag agacatccat tctctgagca
     7021 gaatactcat gactataacc caaaagatac ggtattatct tattaaagcg atatttgtca
     7081 gattcacaac aaaactcctt gccctttata aaaaagctac taactacgta tctttcgaaa
     7141 aatctccaga taaacaaatt caagttattc acttcggtac tttcaaccac cacatcccat
     7201 gatacatcat tcaatattac accattaata atgctcactc catttgagga cgaaaaagca
     7261 tcaacttcag cagtctgaaa taaaatttgc gtatcaatat caataccaga aaaccatatt
     7321 tcagaatcac aattctgaca ctttattgag tgtaaaaaaa cagagtttga gtctatgaat
     7381 tttcctgact ctgtgcggac acagactgga tattttgcgg ataaaataat ttccggcagg
     7441 cctgattgac gtatgagtgc tctttctcta gtatccatcg cacacatagg aacctcctca
     7501 aaggattcta tggacagtcg atgcagggag ctagtccctc cctgcatcgg cgattttcaa
     7561 gttcaaattt taacgtggca ttaaccacat aattaatata ctcgttatgt ggttaattgt
     7621 catcaaatag aaccagatca atattctgtt atttgaataa aattaacttt taattctttt
     7681 ttattcctca tcattttttg gaaaaaatcg ttccatctca gatgacacct tctcccggat
     7741 atcttcaacc atattggcat attcaaactt tggatttccg gagacatgag gactgagaga
     7801 ctcaataccc aaaattttcg ctactgatga ttgtgttttt acaacgcatt caagaagtaa
     7861 tttattaaac tcaatctgat taaacgcgga ctctttacgc tccatctgag cttcatatac
     7921 acgaagcccc agttcaagaa gcattgaagc tgttgctgaa aaactgacat ctttttctct
     7981 tgccccttcc tgtcgacgct tctcaataat cgcatttatt ttttcataag catcattgct
     8041 gatatacagg ttcaccttag ccattagaga ttaccttttt tgaacataaa ataaagttaa
     8101 tttcgaaaca ccagagttca taatgcaaac agggacgcac cgctagcagc gcccctggcg
     8161 gtatcctata aaaaaataca ccgcgccgct agcagcaccc ctaatataaa acaatgtttt
     8221 ttataaaaat agtcagtacc acccctacaa agcggtgtcg gcgcgttgct gtagcggcgt
     8281 taacgacgct gctttaaata aatcagattt aaacaatata aatccacaaa tacaactcaa
     8341 tgatattaaa cgcaaatcag caaaaacttg tttttgcgta gtgtgtggtg cttttggtgg
     8401 tgagaaccac caacctgttg agcctttttg tggagtgggt taaattattt cctgatgacg
     8461 tcaccagagg tgagaaaatg agaaaatggc ttctgacgat ctgcctgttg tttataaatg
     8521 tgacctgcga ggctggtgat tgctttgatc tggctggcag agattataaa attgaccctg
     8581 acttgctgag agcaatatca tggaaagaat cccgttaccg ggttaatgcc atcggtatta
     8641 atccggtaac gggatatggc agcggattga tgcaggtaga ctcccagcat tttaacgaac
     8701 tggcccgcta cggaattaaa ccggaacatc ttacagcaga tccctgtctg aacatttata
     8761 ccggtgctta ttatctggct atagccttta aaaaatgggg agtctcctgg gaggctgttg
     8821 gtgcatacaa cgcaggattc aggaaaaccg aacgccagaa ccagcagcgt cttgcctatg
     8881 catcagatgt ttaccggatt tacagcggga taaaaaacag taaggggctc cagcttcgtg
     8941 catctgaaaa accgctttca aaaatgaaca gctcgcagaa gaattaattt gatatacatt
     9001 ttaaaaaacc ggttatgccg caaatgcggc atggaaggcc acacacaaaa atatcatatg
     9061 agtgataatg ctgactggcc gaagccccgc aaagcgggaa cccatcttat atatatccgg
     9121 taactgcgca gcctcctgcc agaccatttt tcgcgggcac aatatttaca gactaccgaa
     9181 aagccagttg atgaaattga gacatatcag acgacatatc aatgccgcat aatgataata
     9241 taagtaaagc cccgaaaatt tttcggggct ttacttatat tatcatgact tcaactccct
     9301 gaacttttcg gcaatcatcc ataatgcttt gttgattcgt atatcaccgt caatacctgt
     9361 aatagctctt gtccttgtat ttttaccgga ggcacttctt cccgataaac ctccctttat
     9421 catattctcc tgaacccgct gccaggttgt ccagagatcg ttctgtttat cttcccaacg
     9481 gcggggagta attatttgtt caggcgtcac tggcgtttta ttttcatctt catacctgac
     9541 catcagtgca gctctgccaa ataaatgttg ttcgtcactg ttaagatgta tctctttcat
     9601 tgcctccata ttttcagtga ccttatcaaa gacaccgagc acttcatacg caccctcaat
     9661 aacctgcccg acaatatcgc ctttatgtgg aacgcggatt tcgccaaaat tatttccgca
     9721 caccaggcca tttgtgcaga caaaacgaaa aattccgggg atcatctgat aactggatga
     9781 accatcatgt gaattaagca ggataatttc aggaacttcc tgcccgttaa tatgctcttc
     9841 cctgcgaaga cgtaacatat gtttactgta ttcgcggcgt cccagatcgc gaacccgact
     9901 ctgacaggca aagaacggct ggaaaccttc ttcacgtaac ttattgatga tattgattgt
     9961 tggaatatat gtataacgct cactccggga ttcatgttta tcaccggaaa ataccgaagg
    10021 cacaaactgc attaattcat catccgttaa aggacgctca cggcgaatgg aattataccg
    10081 accaaaacgg gaagctaatc gcataacagt aactccttta tgaaggtata aaattaataa
    10141 aaattttttc gcgtaatatc atcctccctc ggttactgtt aacaataaga taacaattcc
    10201 gagggaaagg accatcaaat caggtcagtt cgggactgac caacctgccg ggtgaaaagc
    10261 agtaacagcg cgatcagctc atcagcgttt tttctgacaa gaggggtatg attctccagg
    10321 ctatccagta agtcatgcag ctcattattg acctggtgga gcaccttaaa aagcgcccct
    10381 gaatcctggt tgctctgata ggcgagactg gccttaacag cgttattctg catgatcccg
    10441 atttgtgcga ttgcgtcttt ctgctgatcg cctgttggct gataagtggt gttaaaagaa
    10501 aatgcgctca tgtttcattc tcctgtgtct gatgttttca gcccccatca tctgttgagt
    10561 ttcgggagcc gcggttttga cgttttgcgg gaacctgctc tttattccct ccggtattca
    10621 ccatcacctg tttgagaacc ttcgccgcat ggggcatcac tgccacggcc ggaaacgact
    10681 gtccgggggc agaccgcaaa agtttttgcc tgcttttggg caaaaagttt tgcgggatgt
//
LOCUS       DAZGTJ010000134        10657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000134 DAZGTJ010000000
VERSION     DAZGTJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.134"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            248..748
                     /gene="tssB"
                     /locus_tag="ACHK8E_004598"
     CDS             248..748
                     /gene="tssB"
                     /locus_tag="ACHK8E_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0346767.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            807..2345
                     /gene="tssC"
                     /locus_tag="ACHK8E_004599"
     CDS             807..2345
                     /gene="tssC"
                     /locus_tag="ACHK8E_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0346768.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            2365..3702
                     /gene="tssK"
                     /locus_tag="ACHK8E_004600"
     CDS             2365..3702
                     /gene="tssK"
                     /locus_tag="ACHK8E_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0346769.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            3699..4364
                     /gene="tssL"
                     /locus_tag="ACHK8E_004601"
     CDS             3699..4364
                     /gene="tssL"
                     /locus_tag="ACHK8E_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP0346770.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4377..6029
                     /locus_tag="ACHK8E_004602"
     CDS             4377..6029
                     /locus_tag="ACHK8E_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0346771.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            6087..6578
                     /locus_tag="ACHK8E_004603"
     CDS             6087..6578
                     /locus_tag="ACHK8E_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0346772.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6770..9415
                     /gene="tssH"
                     /locus_tag="ACHK8E_004604"
                     /gene_synonym="clpV"
     CDS             6770..9415
                     /gene="tssH"
                     /locus_tag="ACHK8E_004604"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0346773.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9427..>10657
                     /locus_tag="ACHK8E_004605"
     CDS             9427..>10657
                     /locus_tag="ACHK8E_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0346774.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFD"
BASE COUNT         2422 a         2987 c         3057 g         2191 t
ORIGIN      
        1 agcttaccct gttattattt taagtttcat gccaaattct ctcaccagat aatgccaccc
       61 tctcccgaaa aataatcaag aggccaaaca atatctaaaa tgatacaact gtatctattc
      121 ccctgaaaaa tacattattc atttgtatat tttcctcatc attgcttttt atttaaatca
      181 tccgataatc ccctgaatat aattatgtca ataaccatca gaaaaagtgg atgatgagga
      241 aaaggatatg gccgacagtt tccagaatga agttcccgcc gctcgtgtaa atatcaagct
      301 tgatctgcat acaggcaatg ctaaaaagaa agttgaactc cccctcaagc ttcttgccgt
      361 aggcgattac agtaacggaa aagagcaacg tccgctgtcc gaacgggaca aggttgatat
      421 caataaaaac aacttcaaca gcgtcatggc tgagttttcg cctgcggtta atttaacagt
      481 agaagatacg ctaaacggaa acggtaatga acaaaatatc gcgcttgaat ttaaaagcct
      541 gaaggatttt gaacccgaac aggtagcaaa aaatattccg caacttcgtg tattactcgc
      601 tatgcgcaat ttactacgtg acctgaaatc caatctgctg gataacgcaa cgtttcgccg
      661 tgaactggaa aatatcctga aagacccgac gctcagttca gaactgcgtg atgaactggc
      721 gaaaattgcg ccgcaggaga acgtttaacc cgaacgcgta ttcaggttaa accggagaag
      781 aaaggatccc taaaaaggaa atgctgatgt ctgtacaaca agaatatgcc acctctgaaa
      841 ctgcaacact caccaccact gagtccggcg gcgtttatca gtccctgttt gataaaatta
      901 atttaacccc ggtctcttcc attcaggaaa tcgatttatg gcaaaacagc gaaacgctgg
      961 ccgatgcttc acccgatgag cgcgtgacgg cggcgatcca cgttctgctt tcctgtctgg
     1021 cgaaatcagg cgaggacgtg gttaaactcg acaagagcct gctggatttt catatcgacg
     1081 atttggatca gaaaatcagt aaacagcttg atgcagtcat gcaccatccg gaattccaga
     1141 aagtcgagtc gctctggcgc ggcacatggt tcgtcgtaca gcgcaccgat tttcgcaaaa
     1201 atgtcaaaat tgaactgctg gatatcagta aagaacatct gcggcaggac tttgatgatt
     1261 ctccggaaat cattcaaagt ggtttatatc gccatacata cattcaggaa tatgatacgc
     1321 cgggtggcga acctgtagcc tcattaatat ccagctatga attcgataac agcccgcagg
     1381 atattgccct gctgcgtaat atttccagag tctctgctgc atcacatatg ccctttatcg
     1441 gttccgtcgg accgaagttc ttccttaaaa actcgatgga agaagtcgcc gcaattaaag
     1501 atatcggcaa ctattttgac cgcgcggaat atatcaaatg gaagtcgttc cgcgataccg
     1561 acgacagccg ctacgtggga ttagtgatgc cgcgcgtgct gggccgtctg ccctatggcc
     1621 cggacacggt gccggtacgc agctttaact atgtggaaga ggtcaaaggc ccggatcacg
     1681 aaaagtacct ctggacaaac gcctcgtttg cctttgccgc caatatggtg aagagttttg
     1741 tgaataatgg ctggtgtgtg cagatccgtg gtccgcaggc gggtggcgca gtagccgatc
     1801 tgccgatcca tctttacgat ctcggcaccg gcaatcaggt caaaattccg tccgaagtga
     1861 tgatcccgga aacccgcgag tttgagttcg ccaaccttgg ctttatcccg ctctcttatt
     1921 ataagaatcg cgattacgcc tgcttcttct cagcgaactc tgcccagaaa ccggcgctgt
     1981 atgataccgc tgacgccacc gccaacagcc gtatcaacgc ccgtttgccc tatatcttcc
     2041 tgctgtcccg cattgctcac tacctgaaaa tcattcagcg cgagaatatc ggcaccacca
     2101 aagacagacg cgtgctggaa ctggagctga acacctggat ccgcacgctg gtgacggaaa
     2161 tgaccgatcc gggcgatgaa cttcaggctt cgcatccact gcgcgacggg aaagttatcg
     2221 tcgaggacat agaggacaat ccgggctttt tccgcgtcag actctttgcc gtgccgcatt
     2281 tccagatcga agggatggac gtcaaccttt ctctggtttc ccagatgcca aaagcaaaag
     2341 cctgatcggg cggtggtgtg atgtatgaaa atttgtcgac cactgtgggc tgaggggaca
     2401 ttcctgtctt ctcagcagtt tcagcagcag gcccgctggg aagcgttcag caatgacagc
     2461 attgcgcagc tttgtattcg ccatccgtgg gggatctcaa acgtgctctt tgaccgggat
     2521 gccctgacgc tcggaaagct gaaaacgcag gcagtacgcc tgcgttttgc tgatggtacg
     2581 ctgatcgatt cagacgtggc ggatattctg ccgcctgcct gtgacctgcg cacgctggag
     2641 ggcggcagcg cgattgtttt gctggcgctg ccgcttgcgc atggtaacgg cggtaatctc
     2701 ggtcagggcg agcaggctga aaggccgctg cgctatcgtc aggaatggca aaaggtgcag
     2761 gatatctacg gcagtgacag tgaagatatg gccgttgagc gccatgcact gtccctgcgc
     2821 tttgcccatg ataacaatca ggattacatc acctgtccgc tggcgcgtct ggtgcgggat
     2881 gttcagggca actggacgca ggatgagagc tatattccgc cgttactgac gttaaacgcc
     2941 catgacgggc tggtacagcg cctggacacg ctgctgttgc agctacgggc gaagtgccag
     3001 cgcctgatgg cgatgcgccg ggaaagcaac cagcgcatgg cggacttcgc cgtcgccgat
     3061 gtctctctgt tctggctgct taacgccctg aattccgccg agccggtgct gagcgatttc
     3121 ctgcgctacc cggctgtcca ccccgaactg gtctggcggg agctggcgag gcttgccggt
     3181 gcgctgctga cgttttccct tgagcataac gtcagcgccg ttccgcccta tgtccacgaa
     3241 tcgccgtcca ccgtctttcc gccactgttc tccctgctca gcgagctgct ggaagccagc
     3301 ctgccgtcgc gggtgatcgc gctggatctg gcgtccctgc cgggcaaccg ctggaaagcc
     3361 gacctgcacg atccgcgcct gcaggaagaa gccgattttt acctgtccgt tcgttcctca
     3421 ctgcctgcgc atcaggtact gcaccagcta ccgctggtct gcaaaatcgg tgcgcctgat
     3481 gacgtcacgc tgcttatcaa cgtggcgctg aacggtgttc cactggtgcc actcacctcg
     3541 gttccggcgg cgctgccgct gcggctggag aaccagtatt ttgcgctgga tatgcacagt
     3601 gacgcggcta aatcgatgct ggagtccggg tgctgcatga tttatgcgcc gggaacaatg
     3661 ggcgacctga aacctgaact gtttgcggtg ctgcgctcat gaaaaaagag acggatatca
     3721 atatcgacgt gctgctgcgc gacacgtttc tgacggtggt ggaactgcgt cagggaacca
     3781 ccgtgcgtca cggtatcgag ctgtatcgcc actgtcagcg gcaggtggaa ctggtccgcg
     3841 aacgtctgaa agatgccgga ttcagtcggg agaacgtgga gcacataact tacgcgcagt
     3901 gcgccctgct ggacgaaacg gtgctcagcc gtagtggcat ggacgacggt caggccatct
     3961 ggatgaaaaa cccactccag tcgcatttct tcaacactct gcaggcaggc gagctgctgt
     4021 acgaacggat gaagcaggtg ttgcaggagc ccgcccccgc gcaggcagtg ctgacctgct
     4081 ttcaccgtgt cctgctgctg ggatttcgcg ggcgttatca ggatccggtg gccccggagc
     4141 gtgaccagct tatttcgacg ctcaacgggc tggtcgcgcc atttggcgtg ctgccggaaa
     4201 cggcggtact gaacgtgccg ttatcccccc gtcagcatcc tttgctgcat tcgccgtttt
     4261 tctggctggt aacgctgacg ctgctactgg ccggtgtctg gtggggactg caccactggc
     4321 tgaacgtgct ggtggatgaa ttgctgccgc aaagcctgcg gtaaagggag ccgtgagtga
     4381 ggaacacgct gaaacaggcc atcgtgctgt ggggaatggt gttactgctg gtgctgtggt
     4441 gggtgtttgt cagtccgtct ggcgtactga gatgggccgg tgcagcagtt atcgttctgg
     4501 cggttgccgc attgttgatt taccggcgca ggcagacgtg gacggagatg accggcgatg
     4561 ccgggttgtt atcgctgccg ccggaaacgt accgacaacc ggtagtgctg gtctgtggcg
     4621 atatgtcagc gcatctgttc actgacagcc cggtccggca ggtttcagaa gggctgtatc
     4681 tgcatgttcc cgatgaagaa cagcttgtgg cgcaggtgga gcgattactg acccttcgcc
     4741 cggcgtgggc atcgcagctt gccgtggcgt ataccgtcat gcccggcatg taccgggata
     4801 cggcggttct gaccggacaa ctgcgacggt tcgctcacag tatggcgata gtgcgtcgcc
     4861 gggcgggcgt aaacgtaccc tggcttctct ggagcgggct gtccggctcg ccgttgccgg
     4921 agagagcgaa ttcaccgtgg tttatctgta ccggcggcga agttcaggta gcaacatccg
     4981 cagagaccac catgcccgcg cagtggattg cacaatccgg cgcacaggaa cgcagtcagc
     5041 gactctctta tctgctgaaa gccgaaagcc tgatgcaatg gctggatctg aatgtgctgg
     5101 cggaactgaa cggcccggag gcgaaatgtc caccactggc gatggccgtg gggctggtcc
     5161 cctcgttgcc tgcggtggat aacaacctat ggcagttgtg gatcaccgcc agaaccggcc
     5221 tgacgccgga tatcgcggac accggcacag acgatgcgct gccattcccg gatgccctgt
     5281 tacggcggtt gccgcgtcag tcgggcttta ccccgctgcg acgggcctgc gtgaccatgc
     5341 tgggcgtcac caccgtggcg ggtatcgccg cgctgtgcct gtcagccacg gcaaatcgcc
     5401 agttattacg gcaggtcggt gacgatctgc accggtttta tgccgtcccg gtggaggaat
     5461 ttatcaccaa agcccgtcac ctgtcggtgc tgaaagacga tgcgaccatg ctcgatgggt
     5521 attaccggga aggagaacca ctgcgcctcg gtctggggtt ataccccggc gaacgcatcc
     5581 gccagccggt attacgcacc attcgcgact ggcgtccgcc tgaacaaaaa atggaggtga
     5641 cggcttcgct tcaggttcag accgtgcgtc ttgacagtat gtcgctgttt gacgtcggac
     5701 aggcccgcct gaaagacggc tcgacaaaag tgctggtgga cgcactggtg aacatccggg
     5761 caaaaccggg ctggctgatc ctcgtggccg gatataccga cgccaccggc gacgaaaaaa
     5821 gcaatcagca gttatcgctg cgacgtgccg aagcggtgcg taactggatg ctgcagacca
     5881 gcgacatccc ggccacctgt tttgccgtac agggactggg tgagagccag cctgcggcga
     5941 ccaacgatac gccactgggc agggcagtca accggcgcgt cgaaatcagt cttgttccgc
     6001 gttctgacgc ctgtcaggac gtgaaataaa acataccgcc ggaagaaggc ggtgcttcaa
     6061 tcacactaac aaggagagta attctcatgg ctattcctgc ttatctctgg ctgaaagatg
     6121 acggcggcgc agatatcaaa ggttccgtgg acgttcaggg gcgcgaaggc agcatcgaag
     6181 tggtggcgtt ggatcacgat gtgtacatcc cgaccgacaa caacaccggc aaactgaccg
     6241 gtacccgtac tcacaagccc tttacgttta ccaaagaaat cgatgcgtcc agtccgtatc
     6301 tctacaaagc tgtgcccacc ggtcagaccc tgaaaacggc agaatttaag ttttaccgca
     6361 tcaacgatgc cggtcaggaa gtggagtact tcaacatcac gcttgataac gtcaagctgg
     6421 tcagagtcgc tccgctgatg cacgacatca aggatccttc cagagagaag cataaccacc
     6481 tggaacgtat tgagttccgc tacgagaaaa tcacctggac ttacaaagac ggcaacatca
     6541 ttcattccga ctcgtggaat gagcgtcctt ccgcctgata actgcggcag acaggaaact
     6601 gtctgccttt ttgttgtgtg ctttgtccag tgccgcaggc accggtactg agcaaagcat
     6661 acccacaatc tggtgaaacc tgatgggcct gtgtgaggta acggctatgg gcggtagcgt
     6721 gcctgaacgc ggatcgcagg aatacagaac acgataagga aacccttcta tgacaggaaa
     6781 tcaccccgcc gcgctgctgc gtcgccttaa cccatactgt gcacgggcgt tggacgctgc
     6841 cgcctcactg tgtcagaccc gcgcccatgc ggagataacc attgaacact ggctgctgaa
     6901 actgctggag cagggagaag gcgatatcac ggtgattgcc cgtcgctatg aatgggatat
     6961 cgacacgctc tggcagtctc tgctggcgca tctggacacc ttaccccgct cggtccgcga
     7021 acgtcctcaa ctttctgaac cgctggcagc gcttatcaga caggcgtggc tgatagcgtc
     7081 actggaaggt gacgatccac aaatccgcag ccagcacctg ctgatggcgc tgacagaaaa
     7141 accgatgctg cccgcctgta atgacctgtg ggtattgctg agtctgagcc gcgtgcagct
     7201 tgagcggctg catcccctgc tggatgcgca gtcggatgaa tgtccggcac gtcagccaca
     7261 ggtcaccgaa ccgctgacat ccgcgcaacc agagtcggca acgacggacg taccggcaaa
     7321 aacgctgacg gggaaacagg atgacgccct gctggcggtg cttaaccgct ttaccgaaga
     7381 cgtgacggaa aaagcccgta gcgggcgaat cgacccagta ttcgggcgcg acaccgaaat
     7441 tcgccagatg gtcgatatcc tctcccgtcg ccgcaaaaac aacccgattc tggtgggaga
     7501 accgggggtg ggcaaaaccg cactggtgga agggctggcg ctgcgtatcg ccgaaggcaa
     7561 cgtgcccgac agcctgaaaa cggtgcatat ccgcacgctg gacctcggtc tgttacaggc
     7621 tggcgcgggt gttaaaggtg aatttgagca gcgactgaaa aacgtgattg atgcagcaca
     7681 gaaatcgccg gagccggtac tgctgtttat tgatgaagcc cataccatta tcggcgcggg
     7741 taaccaggct ggcggcgcgg atgcggcgaa cctgctgaaa ccggcactgg caaggggcga
     7801 actgcgcacc atcgcggcga ccacgtggag cgaatacaaa cagtattttg agcgcgacgc
     7861 cgcgctggag cgccgcttcc agatggttaa ggttgacgag ccggatgatg acaccgcctg
     7921 tctgatgctg cggggactga aggcccgtta tgcacagcac cacggcgtgc atatgctgga
     7981 cagcgccatt cagaccgccg tgcgcctgtc gcgccgctat ctgaccggac gccagctgcc
     8041 ggacaaggcg gttgatttgc tggataccgc cggagcaaga gtccgcatga gcctcgacac
     8101 cctgccggag ccgctgacgc agcttcatgc gcgactggcg gcgctggata tcgagcggga
     8161 ggcgattgag caggacagcg tgttttatcc cgaaaccagc ccggagcggc tggcagaact
     8221 gaccgatttg cgtgatgagc tgcaggcaga agccgggcat ctggaagcgc agtatcagca
     8281 ggaaaaggca ctggtgcagc agattatgac gttgcgtcag gaaggaacag acagctctga
     8341 actgcaacag caactgcgga cgcatcaggg ctttgcaccg ctgctggcgc tggatgtgga
     8401 cgcccgcgcc gtcgccacgg tggtggcgga ctggactggc atcccgctct cttccctgct
     8461 caaggacgag cagagcgacc tgctcagtat ggaacagagc cttgaaaacc gcgttgtcgg
     8521 gcaacgcccg gcgctctgcg ccatcgcaca gcggttgcgg gcggctaaaa ccggcctcac
     8581 gccggagaac ggcccacagg gggtattcct gctgaccggc cccagcggca ccggtaaaac
     8641 cgaaactgcg ctcacactgg ccgacaccct gtttggcggt gaaaaatctc ttatcaccat
     8701 caacctctcc gaatatcagg agccgcatac cgtctcccag cttaaggggt ctcctccggg
     8761 ctacgttggc tacggtcagg gcggcgtact taccgaagcc gttcgcaaac gcccgtacag
     8821 cgtggtgttg ctcgacgaag tggaaaaggc gcaccgcgac gtaatgaacc tgttctatca
     8881 ggtatttgac cggggtttta tgcgcgacgg cgaaggccgg gaaattgact tccgcaacac
     8941 cgtgattctg atgacggcta atctgggcag cgaccacatc atgcaactgc tggaggaaaa
     9001 accggacgcc acggacgcag acctgcatga actgctgtat cccctgctgc gcgagcactt
     9061 ccagcctgca ctgatggcgc gttttcagac ggtgatttac cgcccgctgg gacaggaggc
     9121 gatgcgcacc attgtggaaa tgaaactggc gcaggtggtt cgtcgtcttc atcagcacta
     9181 tgggctggaa acggaaatca acgacagcct gtatgacgcg ctgaccgccg cctgcctgct
     9241 gccggacacc ggtgcacgca atatcgacag cctgttgaac cagcaaatcc tgccggtact
     9301 aagtcagcag ttgctggcac agcaggccgc gcaccgtaaa ccagcccaac tacggcttgg
     9361 ctgggatgag gaagacggga ttgtactgga gttcgctacg gaagagatgc aataacggag
     9421 gcttttatgg gtattcagaa tttcgaccac tcacaccaca agctcaaaat tcgcggcctg
     9481 aaaagcccgg tggacgtgct gacgtttacg ggccgggaac aactcagcgc acccttccgc
     9541 tacgacattg aatttaccag taccgataaa accatcgagc cggagtcggt gctgatgcag
     9601 gatggcgcat tcagcctgac ggcaccgccg gtacagggaa tgccggtaca gactgccctg
     9661 cgcaccctgc acggcgtcat taccggcttt aaacatctct catcgtcaca ggacgaagcc
     9721 cgttacgaag tgcgccttga accccgtatg gcgctgctta cccgcagccg ccagaacgcc
     9781 atctaccaga accagacggt gccgcaaatc gtcgaaaaaa tcctgcgcga gcggcaccag
     9841 atgcgcggtc aggacttcgt gtttaacctc aaaagcgaat acccgtcccg tgaacaggtg
     9901 atgcagtacg gtgaggacga cctgacgttt atttcacgcc tgctgtcaga agtgggtatc
     9961 tggttccgct ttgccaccga cgcccggttg aaaatcgaag ttatcgaatt ttacgacgac
    10021 cagagcggct acgagcgcgg gctgacgctg ccgcttcgcc atccttctgg gctgtttgat
    10081 ggtgaaacgg aagccgtatg ggggctgaac accgcgtaca gcgtggtgga aaaaagtgtc
    10141 tccacgcggg actacaacta ccgtgaagcc acggcggaaa tgaccacagg ccagcatgac
    10201 gccaccgggg gcgataacac cacttacggc gaagcctacc actatgccga caacttcctg
    10261 caacaaggcg ataaagaggc agcggaaagc ggcgcgtttt atgcccgtat ccgtcacgaa
    10321 cgctacctga atgaacaggc cattctcaaa ggccagagca ccagttcact gctgatgccg
    10381 gggctggaga taaaagttca gggcgacgac gcgcctgcgg tgttccgcaa gggcgtgctg
    10441 attaccggcg tgacgaccag cgcggcgcgt gaccgcagct atgagctgac gttcacggcg
    10501 ataccgtact cagagcgcta cggctaccgt ccggcgctta tcccccgccc cgtaatggcc
    10561 ggaacgctcc cggcgcgggt caccagtacg gtgaagaacg acatctacgc ccatatcgac
    10621 aaggacgggc gctaccgggt caacctcgac tttgacc
//
LOCUS       DAZGTJ010000135        10270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000135 DAZGTJ010000000
VERSION     DAZGTJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.135"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(427..1800)
                     /locus_tag="ACHK8E_004606"
                     /pseudo
     CDS             complement(427..1800)
                     /locus_tag="ACHK8E_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1999..3045)
                     /gene="fbpC"
                     /locus_tag="ACHK8E_004607"
     CDS             complement(1999..3045)
                     /gene="fbpC"
                     /locus_tag="ACHK8E_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP0346775.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(3057..5135)
                     /locus_tag="ACHK8E_004608"
     CDS             complement(3057..5135)
                     /locus_tag="ACHK8E_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0346776.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(5204..6235)
                     /locus_tag="ACHK8E_004609"
     CDS             complement(5204..6235)
                     /locus_tag="ACHK8E_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0346777.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(6232..7548)
                     /gene="uhpC"
                     /locus_tag="ACHK8E_004610"
     CDS             complement(6232..7548)
                     /gene="uhpC"
                     /locus_tag="ACHK8E_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0346778.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7621..9162)
                     /locus_tag="ACHK8E_004611"
     CDS             complement(7621..9162)
                     /locus_tag="ACHK8E_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0346779.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(9162..9791)
                     /locus_tag="ACHK8E_004612"
     CDS             complement(9162..9791)
                     /locus_tag="ACHK8E_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP0346780.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            complement(10067..>10270)
                     /locus_tag="ACHK8E_004613"
     CDS             complement(10067..>10270)
                     /locus_tag="ACHK8E_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0346781.1"
                     /translation="ATGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAL
                     YIDGRREMMQWYADYIDSLSEQT"
BASE COUNT         2566 a         2746 c         2663 g         2295 t
ORIGIN      
        1 ccgctcgtgg acagagagcc ggggccggtt atggtgttta atggcgagaa aaatgttcct
       61 gcagacagcc ttaagtcaca gagacttatt tatcctgaca gtaataatca tacaggtaag
      121 tgaatggtat ctgacgatga ctggtaaatg atatattcct gctttttata ataattttat
      181 taaagttgag ttgtacaaca atgtgggaga gaaataatct gaccggatta cagctatttg
      241 atgagtttga gtacgatatt gatattggtt tcagttattg atttatttat gtcataactt
      301 ttctgaaaga tagctggtaa ttgccttgag aaagttttta tatgttattc tgtaaaacag
      361 gataacagaa aataattatg tctgaaataa gtagatgatg tcccctctga gctggctgtc
      421 tcttgatcag atatcctgat caagagactt catcaccagg taaccctcaa ccatatcctg
      481 aagtctgaac cagtcatccc acataactac ccaaccgggg cggcctgtgc gtttgctgtc
      541 atgtcatcgc cccagtttag ccagtttcag actggctcat ttcaggatcg gtgcctgcgc
      601 cggaagcggc tctccttcca gcttaaccca cagcagtttc cactctgtcg gcgtcagtat
      661 tttctcacaa ctgtcatttt gtgtttcttc actgacacct ccctgccgca ggcccggcac
      721 ccgtaccgcg ataaacgcct tgataaccac catgcgctca aggttatccc gggtctgaat
      781 tcgcagcgat tccacacatg taccaccact tttccacgcc ttgtggtatt cctctatcag
      841 ccagcgtcgc tcgtaatggc tgacgatacg tcgcgcatcg gcggcactcg ccactttttc
      901 tgacgtcagc agatgccagc aggcactgtc ctctgcctgc tcccggcaac agacatacgt
      961 gagcgagagc gcctggccgc tgttgtcggg attttttatg ctgacttcgc tgtaactgat
     1021 gaacatccgg gcctggcggg ctgcccgccc gcctttttgc atcacattca gcgtgtggct
     1081 tcccgcggtt gccaggactt ccggcagttc gaagagcttg ccgggtgctt cttccagccg
     1141 gctattctgt gcagcacgca ccacgaagcg ctgtccgtgg ctgactttat aatgcaggta
     1201 atgccagata tccgcttccc ggtcacagac agtgattacc cgtttctgta tctcccccag
     1261 ccgttcggcc atgcgctccg aagcctgctg ccagcggtaa ctttcttttt cttcacaggg
     1321 acgttctttt cgctggtgct taacaccgta ggtgtccgtg gcccgactcc agctctgctg
     1381 ttcgataaga gcgactggca gggcgctgtc gggggcgtat atcaggacag agtgagccag
     1441 cagcccgcgc gtcttcgggt tagtggtggt attccccagg tcatcagatg ccgtactgtg
     1501 gctgaagtta atggtggagg tgtcttccag tgcggggagc agcggatgtg cctcacatgc
     1561 ccttgcagtg gcggtaaatc cggctccgga aatggcttcc ggggacactg acgggttacg
     1621 catcaggcgg tacgcacctt caacctgagc agtggagtgg gattatttca caatagaaag
     1681 acctgcatgc tgagcgagag aagaggccag tgacacaagg cgtcgtgtac gacgcggatc
     1741 accgagacgg gcatgtccag actgctcgtt agcccatgaa taacaatcag aaagtaccat
     1801 aacagacccg aagaaaatga aatataagag aagatcaacg ggtgaagaaa aagttcaaaa
     1861 aatcgctgcc ggggaggaag gaaactaccg gatggaaaga gtccccccta aagcagactg
     1921 acagacatca caaatcccca ggtggtgatt tgtgtataag agacagctga gctggagggg
     1981 accgcttatg cgctattttt atgccgcatc cgctaaaaca aacatcccgt aagggtggat
     2041 ttcgaggtaa tagtgctcac caatatcggg ctgtaaacgg gtggcgttga cttgcaataa
     2101 aatctcctga ccatgccatt ctacggtcac ttcgtactgc ggccccatgt aagcgacatg
     2161 gcgaataaca caacgctgac tctcttcgcc gtgattgttt aaggtgatgg cttccgggcg
     2221 aacgccggcg gtgccgctac cttgcgccgg gaaatgcgct gcgcgcggca ggcgataacc
     2281 gtagatatcg acatactctt cgctaaagtt cgccgggaac aggttggcgt cgcccataaa
     2341 acttgccata aatcgggagg ctggctgacg atagagatcc tgcggtgagc caatctgcat
     2401 gatgtgacct ttattcatca ccagcacagt gtcggacacc gcgaaagctt cactttgatc
     2461 gtgagtcacg tagagtgacg tgatattaaa ctgcttttgc agctcgcgga tcttatcgcg
     2521 catgctgcgg cgcaggttgg catcgaggtt acttaacggc tcatcaaaca gcagcacctt
     2581 tggcttgagg atcaacgcac gggccagagc cacacgctgt tgctgaccac cggaaatctg
     2641 gtcgacatag cggtcttcga atccttccag atccaccatt gtcagcgcat ctttcacccg
     2701 ctgcttcact tcactacgcg acacgccaag cattttcagc ccatagccaa cgttttcgcc
     2761 cagcgacata tgcgggaaca gagcgtatga ctgaaacacc atgcagatat cgcgttgctg
     2821 aatggaacga tgcgtgacat cttcgccatc tataaaaatt tgcccttcgc tcggtttttc
     2881 cagcccggca accagacgca gaatggtggt tttgccgcat ccggaaggac cgagcagcgt
     2941 caccatttgc ccttgtggga tggtgagatt gatattgtcg ataaccctat tactgccgaa
     3001 tcgtttagtg acgttgcgca gttccacaaa atttttctga ctcatagtgc gctccattac
     3061 gcctggtttt tggctttaga acgggaaata cgtgcttcac cgatcagcca gtcaaagagg
     3121 aaaataatcg ccagcatcac cacgatcaga atcgaaccgt aggcgatggc tacgccatat
     3181 tcgccgtctt ctacgcggtt aaggatgtag gcggttgcca cgcgtgtgtc cggtgtgacg
     3241 agaaagacga tggcgctgac tgtagtgatg gcccgcacaa agctgtaaat cagggccgag
     3301 agaatggccg ggcgtaacag cggcagtaaa atgtgcgtaa tggttcgcag ggaacccgca
     3361 cgcaggctaa gtgaggcttc atccagcgat ttgtcgatct gccccagacc tgcaatcccg
     3421 gcgcgaatac ccaccggaac gttacgcatc accatcgaga taataacaat tgctgccgtt
     3481 ccggcgagat agagtggggc gctattaaag gcaagaatat acgagacacc cgccactgta
     3541 cccggtacgg caaagcacag catggtggtg aactcgatgg tctttttgcc tttgaattgt
     3601 tgacgcacca ccacccaggc gatgagcagg ccaaagatag cggtaatggg ggctgcaatc
     3661 ccggcgtaga gcagggtatc cagcagtgaa ggccacgcgc cgtcgctcat cccctgacca
     3721 aataacttga taaagttatc cagcgtcagg gtgtagtcaa cgccccagtt caccgtgaag
     3781 ctgccgtaga aaatgctgcc gtagagcagg gcgttaaagg caatccatac cgtaagtatg
     3841 gcgaccacgc tccacaccag ggtgaccggt agcggctgga cgtcgccacg gtaggatttg
     3901 ccggaaaccg tgacgtaaga gcgtttacca atccacatat actggacgca gaacaccagc
     3961 agcgagaaca gcagcaggaa agcaccaagc gtgctggcgg cctgataatc cagctgcgag
     4021 ccggtaatgt agaagtaaat ttgcgtcgcc agtacgtcga agttgccgcc aagcaccagc
     4081 gggttactga aatcagccag tgactgcacc acgacgatta aaaaggcatt ggcgagcgcg
     4141 ggctttagca atggcacaaa cacgccgtta aaggtttgcc agcggctggc gcgcagggta
     4201 taggaggctt cttccagcga gggatgaatg gttttaatgg ccccgtcgag gatcataaaa
     4261 gccattggcg tgaatgccag aacctgcgcc agccagatcc cggtaaaacc atacagccag
     4321 ttggtgttgg tcagcccaaa ccactccacc ataaactccg ttatgtagcc ggagcgcccc
     4381 atcatgagcg tcacgcctaa gccgaccaca aaaggtggcg taacgatggg cagaatggaa
     4441 aaaatgcggc caataatggc gctgcgtttg gcgatgcgcg tggtataaat cgccagcacc
     4501 aggccgaaga aggtacagcc aatacccacc gctatcgaca agacgataga gttgacgatc
     4561 acctgcacaa tatgtgcctg cgagagcacg ttcataaacg ccagcggggc gaactctccc
     4621 gcgtcgttgg tgaacatcgg gataaaaatg gcgatgctcg gccagacgat aaaaatgcca
     4681 atcagcgcca caatcgcaat cagtgagccg atcacgaagc ggtcgccgcc cagccattcg
     4741 aggcgggtta aggccagcgt cataatcgcg cctaattcaa caaacagcac gatggcggcg
     4801 tagcctaaac cgcgtccagc cacggtggcg ctgatgacga taaacgccat acagagcaat
     4861 gcccagctgg catcaaaata atgacgacta cgctgttcgc gtgtggcctc acgaaacgga
     4921 cgaaccagca acagactcgg taataaatac cacagccagc tgatattaaa atgcgaccag
     4981 ccataagcct cgaggatctc atcactcgtg gactccagca ggccataatc aaggctccag
     5041 ctcggcaaca ggataaatgc cagccagccc agcaaaaccc aaaggaaaat cgcatcccgc
     5101 tttttcacgg gatggagtgc aagtgtctgt gacatagcag ttccggtatg gttggccggg
     5161 gcaggcgttt gtgctgcacc cggcaaatgc gcctaatgcg ggtttattta cccatcttca
     5221 cttcgctgac ccatttatta atcaatgcct tacgaacatc cgtggaacca tatttatcca
     5281 tgtcgtagtt gattaatttc aggtcgtcga gcttcagcga atttggcgac gtttcggcgg
     5341 tggtattggt caggatctga taagacttcc ctttcttcca cgccagttcc tgagcttctt
     5401 ttgataatac ccagtccaca aacagtttgg cgttgtcgag gttgcgcgcg cctttcagaa
     5461 tgctgacgcc gccaatttcg tagcccgtac cttcacacgg ggaaatcaac tccagcggcg
     5521 cgccctgttc tttttccagc gagtagtcat gcaggaagcc aataccaatc gccgtttcgc
     5581 cacgggcggc gttacgtgcc ggggctatgc ctgatttggt gtactgggag acgttggcgt
     5641 tcagttgttt tagataatca aaggcctgat cttcccccca cagctgcgca aaagtagcca
     5701 gggcggtgta ggcagtaccg gaactttgcg gatcggcaat ttgaatttcg cctttatatt
     5761 ccggtttggt gagatctttc cagcacttcg gcaccggcag gtttttctct ttcagacgat
     5821 cggtgtttac gccaaaaccg aggatcccca catatacggc agaggacaga ttccctttca
     5881 cttttgccgg atcgcggaat ttctccatga tctgctcaag gttaggggat ttatacgcct
     5941 gtagcaggcc catctcgcca gcctgcgatt gcgggtctaa agtgccgccg taccagacgt
     6001 cagcctgggg gtttttcttt tcagcatcga cttttgccag ggtgcttccg gaaccgttgc
     6061 ggataaacga ggtttttaca tcatatttct cgccaaacgc tttggtttcg gcctcacaca
     6121 tttcattggt ggcgctgcaa tagaccacca gccggccttt cgcctgcgcg gcacccgtta
     6181 aagtcgccag cgcaacggca gaagcaatca gagtagaggt aaaggtcatt ttcattgttt
     6241 tatccttcga gcgtgagtgg tggtgatgcc cgccatcagt aacggcatca gcagtaaacc
     6301 cataagtact gcggcgaccg agagtaaact gaacattccc gaccagccgt agcgttcgat
     6361 gaccagcgac agtggccagc ccgcaagcgc cgcgccaagg taagcgaaca ggccgagaaa
     6421 tccggtgatt gaacccgccg cagctttgtg cccacactcc accgccgcaa gtccaatcag
     6481 catttgtgga ccaaagacga aaaagcccac ggtaaaaaag cacactgcca gcagagcgta
     6541 gtgatgaacc ggtgccagcc acagtgcggc gactgacacc atcaacccca gcgtgaacag
     6601 caaaatcatt ggcgctcgtt gcccgctaaa cagcaaatcc gatccccatc cggcaaataa
     6661 cgcgccgagt aagccgccca cttcaaacag cattaccgtg gcgttggcgt tgagcaggtt
     6721 gacgccgtgg ctttccgtca gccagatatt gccccagtcg ttaagcgcaa tccggatcac
     6781 ataaaccagt acataagaga cgcccagtag ccagatcagc ggattttgca gcatggtggt
     6841 gcgcaacatt tgccacagcc ccatcggcgg actttgttgt tcctgacgta actccagcgg
     6901 atcgtgacgc cagtgaccaa ctgtcggtaa tccctcttcc tggggcgtgc ctttgagttg
     6961 tagcgttaac cagatcccta atgccatgct gattatcccc ggtgtcagca tcgcggcctg
     7021 ccagccccac cagtgggcgg caaaggcgct aatcagcgga ataatcgctc cgccaatgtt
     7081 gatcgacata ttccagcagc cccaccagaa accgcgctca ttgcgtgagt accagtgggt
     7141 cagcaagcgg gcacacggtg gccatcccca gccctgaaaa aaaccgttca gtgtccagac
     7201 cacaagcaga agcgtaagtg attcgccaaa ggcaaacacc acgttcaata acccggtggc
     7261 gaacaagccg acgcccataa aaccgcgttg tccgtggctg tcatgccaca gtccggcggt
     7321 aaatttcgac aatccgtagc tgagataaaa cagcgaaccc agcaggccaa tgtcgccttt
     7381 atccagtccg aggtcggttt gcagcgccgg aaggacgtaa ttcacgcttt ttcgcgtcag
     7441 ataaaatgcg gcgtagccaa taatcatgca catcagcaat cgcggacgta atgtccggta
     7501 gcattgatta atttctctcg ctgaacgcgc gtgcatggtt gtctccatgt tgctgactat
     7561 atgaaaatga aagtgaaagg ggatgagaga aaatccggga tacctaagac tttttcctgg
     7621 ttatccggcg atttgttgca aaaatgtggg caagttaaca attacccgcg tgccgtgctg
     7681 tttttcgagc gttaagtcgc cgcccagggc gctgacgcgc tcacgcatcc cctgaatgcc
     7741 aaatccggcc attttcccgc tggcgatccc cacaccgttg tcgctgacct ccagatgcaa
     7801 tacctctccc tgctggcgca ggataatcgt cacttcgctg gcttcggcgt gtttacagac
     7861 gttattgagc aactcctgca ataaccgata caaggtaaag cgcaccgttt cgttttctgg
     7921 tgtggagttc agttgatagg caaactggca atgaatgccg cgttcactaa aggcaaattc
     7981 attaatcaga tgcagcaacg cttcgcggaa tgtcagttca tcaagcgcgg gaggccgcaa
     8041 ctggcgcagc aactggcggg tggagagatg aatgcggcgg gcgagttcat tgatttggct
     8101 ggctgcggat tgaatttgcg ccggatcgcg cgcccgcttc accagttgcg actggatctg
     8161 aatggcggtg atgttctgac cgatttcgtc gtgcagctct cgcgccaggt ttttgcgcgt
     8221 gtcctcctcg gtgtggatca gcttttccgc caatgcccgc cgcgctgcca gctcggcctc
     8281 cagccgttgt cggtaatggt gcaggtttag cgccagatgt tgctggcgac tgatggcaat
     8341 gcccaggcca attcccagca atgcctgggt cgcgaggaaa atctccagct ccaccaggtt
     8401 actaaagccc acgccaatct ggcgggcgat ggtgatcatc atgctgccca aaagcccgga
     8461 gagtacgcca ccttgccagc caaatttcca cgccatcacc acattgggta gaaaaactac
     8521 gatcaacagc aggcgttcaa tttcaggtga cagcaccatc tgagtgccga taccaatgat
     8581 aaaaaacagg ctacaccaga tgattaaaga ggtacgcaag gggggattat gcgtgtcaag
     8641 ccccaggagc tggtagcgat gctgctggcg cagaaactca aagatgagat agacaaacgg
     8701 cgtcagtaat acgccgccgg taaatgaggt caaccccggc agcattattg ggcctgacat
     8761 aaaaggggag agcagggcgg tattcaacaa tgaagctgcc gtgacagtgg ctaacagcaa
     8821 cgtcagacgc tgccagtaaa gtggaaaacg gtgccagaag ttttgcgcaa ggatggcagg
     8881 aaacaggcta ataaccggcg ttgccagcat gagataaccg ttcaatagct gttcactatg
     8941 gagccagaac atcatcagca aaggtgggag caccagcgca ggccagtagc ggcgagagag
     9001 caaaataaga agggcgaggt aaaccccctg cgggagaaac aacaccgcct gttgaccgtt
     9061 attggtcaga taaaagctga gcgtccacag catcagccag ccagttcccc aggccagcac
     9121 aataaacaga gaaatcaccc aatgacgaag gttgcgcgac attaatgccc cgccagcaga
     9181 tggtggtcga gcgcaaaatg caccagctca atggtactat ggcagttcag ttttcccagc
     9241 acattggcgc gatgcacatg taccgtttta tgactgagtt cgagcttaaa ggcgatctct
     9301 ttaacgctgt cacctttgac cagtaaatca aacacttcac gttctcgtgg ggtgagaatt
     9361 tccagcgcct gggcgggttg cccgccgccg cgtaaagccc gaattgcatc ggcacagaga
     9421 taatgcccgc ccagcccgac cgagcgcacc gcctgtacta actcttccgg cccacagcgt
     9481 ttggtcaaat agccgctggc accagcatcc agcgcgcttt gcacaaaggt tggcgcgtca
     9541 taaatgctaa gaataatggc gcgaaattgc ggtttttgtg cacgcaggcg cttaagcaaa
     9601 ctgaggccgt tttcgtccgg catagcaata tcaatgacgg caacattgac gtcatcacgg
     9661 attaatgccg gccatgcctg tgcggcacta ctgtactgac caatgacctc cagatcgtct
     9721 tccagagaga gtaattgtgc gaagccagag cgcaccacca catggtcatc caccagcaca
     9781 acccgaatca tacttttctt ctcttattat ctcattcttc tatcatgcat cacggcgcgt
     9841 cgctggcaat caaccagcga cagatatgga atggatgtaa caaaaaatgt aatttgttat
     9901 tcaatataat tatgctttat ttcatgagtg gctctatatc tggtgttcag attattgtct
     9961 gcaggggcag ttcagaagat gtgagcaaat aaaaaagaaa acataaataa aaccatccgg
    10021 ctttcggggc cagatggtta ttcattattt ttatgtcact gattcatcag gtttgctcag
    10081 aaagcgaatc aatgtagtca gcataccact gcatcatttc acggcgacca tcgatataca
    10141 gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac tgcatttcaa
    10201 tccaggcaga attaaacccc tgttcgtgca gaatggtact catggtgtga cgaaaaccat
    10261 gaccggttgc
//
LOCUS       DAZGTJ010000136        10135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000136 DAZGTJ010000000
VERSION     DAZGTJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.136"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(348..770)
                     /locus_tag="ACHK8E_004614"
     CDS             complement(348..770)
                     /locus_tag="ACHK8E_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0346782.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFHTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            891..1169
                     /locus_tag="ACHK8E_004615"
     CDS             891..1169
                     /locus_tag="ACHK8E_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346783.1"
                     /translation="MKKCVTVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLEQQKKSLR"
     gene            complement(1261..1506)
                     /locus_tag="ACHK8E_004616"
     CDS             complement(1261..1506)
                     /locus_tag="ACHK8E_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346784.1"
                     /translation="MKRRKLTPAEEKRLADAFVATAIAMGLKGNKKLPQEIKRRVRAQ
                     VLSAIENERKAQAAHDARRQEFEAGENTFTWQPLRRR"
     gene            1627..3678
                     /locus_tag="ACHK8E_004617"
     CDS             1627..3678
                     /locus_tag="ACHK8E_004617"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HFP0346785.1"
                     /translation="MGIEIIGDNTTITSIKPKGKVVYCRGESHKYWLELQFVDENNVP
                     VTGLNVWLEYHPLASAQEVAMWKKYGNDYNPTPPPNPPAGVTDNQGVVRFDNLYWIAV
                     DVKTDAQPLTDEMEKRPLGLRRNPNSQPVSNNRFRPETRDPEWRSDVQEKAEAAGYIH
                     HYVTIGELCDQLPEIEGWTEPEPPKFHFPHGTLLKGTEITREALDKRHVIEICPFRAW
                     SLVLHDTKDYSLANGYNLGVMADMAYADKVLIEDFFRRKCLDLSTTPRHEVHPFYPHA
                     LVVDVPFRERYTTVEFLDSRSQKDKAPSRLLIDSTQLFYVENASHVIVAWRGTQEVPD
                     WVTDGSYSSQPCPPELAGAGRIHGGFWDAYHLVKVRFEDDFKKIKLTLANANGAKKLF
                     IAGHSLGGALGLIYAAEMKVVEPILYTYGMPRTFTADAVGNLYQITHYRHVNDSDVIA
                     TVPFDANLDNWLYGKFGPLGSTLGFFWTLLVELPVQMTGAYTGEHFWHHGKPVVFFRA
                     TQTGSYEECKTMNSHTCRRLSFSQQYKIKLFLVPSLAEVENEEAKEAQEAFVREFPVE
                     DIQRIFPRNTNPRMDHMPSPFRHSMAGEYLPFLNNQLLESAGAEQELQRKIYREDFRQ
                     QMKQFGDSAPPEEMKRNRIFLALQDMVGNTLDQTRKMPGGENALIRFKSVAEEEVESS
                     R"
     gene            3662..4453
                     /locus_tag="ACHK8E_004618"
     CDS             3662..4453
                     /locus_tag="ACHK8E_004618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346786.1"
                     /translation="MSRLVNIVAGMLPFLALLPLSGCASRPDYALSPSGNTEWVTVAV
                     KLPPETEALPVDVLYRSEICRRKDYDPTTESHVRTIRGFNPVQIPLSPPDNSGFRKVR
                     IALDGGTRCGWTLSGVRVGIQMSRSSALAQGKDVTAANYVFDFDDEGYGSAFGKGKPL
                     SVSGDLFFKTEFFPLVYINHMFNETYLELFAGNAKYEKWNRYYRVRETENIAIEPVLY
                     VNKVVRVESAKGKENGPGMIVSYPDASTEKIKDIYPDYTKLLSMK"
     gene            complement(4955..5911)
                     /gene="iroE"
                     /locus_tag="ACHK8E_004619"
     CDS             complement(4955..5911)
                     /gene="iroE"
                     /locus_tag="ACHK8E_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HFP0346787.1"
                     /translation="MYASEYRSTRPRKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDDTRHYRVWTAVPNKTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAEGRKTDPHSGHFSRKSGGSNDF
                     RHLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDTWLSSSYFRLYYSASPSLGRG
                     NDALLSRVTAVEPLQFCAKHLAIMEGSATPGDNRETHAVGVLPKIHTTLTILKDKGVN
                     TAFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            6364..6615
                     /locus_tag="ACHK8E_004620"
     CDS             6364..6615
                     /locus_tag="ACHK8E_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0346788.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            6612..6899
                     /locus_tag="ACHK8E_004621"
     CDS             6612..6899
                     /locus_tag="ACHK8E_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0346789.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            7067..7471
                     /locus_tag="ACHK8E_004622"
     CDS             7067..7471
                     /locus_tag="ACHK8E_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFP0346790.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHAWDDYVDNFFR"
     gene            7780..9264
                     /locus_tag="ACHK8E_004623"
     CDS             7780..9264
                     /locus_tag="ACHK8E_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0346791.1"
                     /translation="MRKIIVHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGEDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(9485..10093)
                     /locus_tag="ACHK8E_004624"
     CDS             complement(9485..10093)
                     /locus_tag="ACHK8E_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0346792.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATKS
                     VSSGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEG
                     GGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNL
                     DGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         2680 a         2166 c         2483 g         2806 t
ORIGIN      
        1 accattgaaa tgttttatat tttgagatag cactgcgata gtatttgatc agaaactaat
       61 gggtactccc agatgcatat tcaggttgat ggaaggggat aaaaaatccc cgtgcagaat
      121 cttccatccg gtgggagaaa acttgtcatt ttgacctgtt cgcccttcgg aacggtcgaa
      181 accgatcgcg catcgctttc atgcatagtt atgcaggccc ctgaaaacga ttctgacgcg
      241 ttttttctgt ttagcctggt ttttttgtct ttttgcgttt tttgcgtcag aacgcgtctg
      301 agagcgtttt aaggggtacg tacaattaag ggattatggt aaatccctta ctgtctgccc
      361 tcgtatccat cgagataaac cgcagaaatg gtgtctgcca ttagtttcat ttttttcggc
      421 gaaggcatat cgctgtttgt gtattttctg ccagcaaagg ttgttgtgct ccggagtgag
      481 tgaaaaaatt tttctgagtg gaattctttt acacgttgct gtgccgtttc tgctgatact
      541 ccggctgctt tgtctttcat ccccatatcc cggaactgct gaaagaacgg gagcatcata
      601 cgtatggtgt ctgctttctg ggtgtggaag ttaggatcaa atccatcatc tgaagcataa
      661 acataatgcc gggtatgtcg ttccaggctg gtctttggtg tgcatcctgt aagagctatt
      721 gttgctgtta tcagaggtat cagaagcagc ctggcagcgg cagatctcat aaacatctcc
      781 ttgcgttttt aatcttgata ctcagggagt tatatttccg aagagtatct cttatcatac
      841 gtcggtttac cccaatgtga tttgatggtc aatgagtcag gaataatccc gtgaaaaaat
      901 gtgtgacagt cctgcttttt agtgttcttt ctgcttcggt tgccagtcat gcggacagca
      961 atgtttttca acaaaaggcc gagaagacaa aaaacggcat tgttgcggat gttaatgcca
     1021 gacagaaaat gttcagtaac agactgaaga tacaacttca gatcgatgag ttgagagcag
     1081 agctggctgg caccagagat gcccaaaaaa gagaggattt gcgtaaaaaa ctggagtctc
     1141 tggagcagca aaagaaatcc ttacgctgat gggggcgtat tttccccggg catcatggtc
     1201 ttgataccat ttatttttat gtggtttttt tggtgggttt tcaagcggtg aagatgtttt
     1261 ttatctacga cgtaatggct gccaggtaaa ggtgttttct ccggcttcaa attcctgtct
     1321 gcgtgcatca tgtgctgcct gtgcttttcg ttcgttctca atggcactga gaacctgtgc
     1381 ccgtacccgc cgtttgattt cctggggtaa ttttttattg cctttcaggc ccattgcgat
     1441 agctgttgca acaaatgcat ctgcaagacg tttctcctct gccggagtca gttttctgcg
     1501 tttcatgaag gcctcctgtt tgcacagatt aacgtaacat tatgtgccat atgaggaaat
     1561 tcaggttaag cgcggtcccg ggtatgatgg actttgttta accattttta gtgcagggat
     1621 aatgtcatgg ggatagaaat tatcggtgac aatacgacca tcacctccat taagcccaaa
     1681 ggtaaagttg tttactgtcg cggagaatcg cataaatact ggcttgaact ccagttcgtg
     1741 gatgagaaca acgtccctgt taccgggctt aatgtatggc tcgagtatca tccgctggcc
     1801 tcagctcagg aagtggcgat gtggaaaaaa tatggcaatg actacaaccc cacaccgccg
     1861 cccaatccac cggcgggtgt tacagacaat cagggggtgg tgcggttcga taacctgtac
     1921 tggatagcgg ttgacgtgaa gactgatgcc cagccactga ccgacgagat ggaaaagcgc
     1981 ccgctgggct tgcggcgaaa cccaaacagc cagccggtaa gcaataacag attccgaccg
     2041 gaaacccgcg accctgaatg gcgctcagac gtgcaggaga aagccgaagc tgccgggtat
     2101 attcatcact atgtgaccat tggagagctt tgcgaccaac tgccggagat tgagggctgg
     2161 actgagccgg agccgccgaa gtttcatttt cctcatggta cgttgctgaa aggtacggaa
     2221 atcacccggg aggctcttga taaaaggcat gtgatagaaa tatgcccgtt ccgggcatgg
     2281 tcactggtgc tgcatgatac gaaagactat tcactggcaa acggctataa cctcggagtg
     2341 atggctgata tggcctatgc ggataaagtg ctgattgagg attttttcag aagaaaatgc
     2401 cttgatttat ccaccacgcc ccgacatgaa gtacatccgt tctatcctca cgcactggtt
     2461 gtggacgtgc cattccgtga acgctatacc acagtggagt ttctggactc ccgaagccag
     2521 aaagataaag cgccatcccg tctgttgatt gacagcacac agctttttta cgtggaaaat
     2581 gcgtcccatg tgatagtggc atggagggga acacaggaag tacctgactg ggttactgat
     2641 ggttcttatt cttcacagcc ctgtcctccc gaactggctg gagccgggcg tattcatggt
     2701 gggttttggg atgcatatca tctggtgaaa gtgcgattcg aggatgactt taagaaaatt
     2761 aaattaacat tagctaatgc taatggtgct aaaaaattgt ttattgccgg gcacagcttg
     2821 ggcggagcat tggggttgat ttatgcagca gaaatgaaag ttgttgagcc aatactctac
     2881 acctacggga tgccgcgcac ctttactgca gatgccgtcg ggaatctgta ccagataacc
     2941 cattaccgac acgtaaatga cagtgatgtg atcgccacgg tgccgtttga tgcgaatctg
     3001 gataactggc tgtacggtaa gtttggccct cttggcagta cgctgggatt tttctggaca
     3061 ctgcttgttg aattaccggt gcagatgaca ggggcgtata caggagaaca tttctggcac
     3121 catggcaagc cggtggtgtt cttccgggcc actcagactg gttcttatga agaatgcaaa
     3181 acgatgaaca gtcatacctg ccgtcgtctc agtttcagtc agcaatacaa aatcaaacta
     3241 ttccttgttc cgtcactggc tgaggttgaa aacgaagaag cgaaagaagc tcaggaggca
     3301 tttgtccggg aatttccagt tgaagatatc cagcggattt ttccgcgaaa tactaatcca
     3361 cggatggatc acatgcccag tcccttccgc cattcgatgg ctggtgaata tctgccgttc
     3421 ctgaataatc agttactgga atcagccgga gcggagcagg aattgcagcg gaagatatac
     3481 cgggaagatt tccggcaaca gatgaagcag tttggagaca gtgcaccacc ggaagagatg
     3541 aagcgtaacc ggatattcct ggcactccaa gatatggtag gaaatacgct ggatcaaacc
     3601 cgaaagatgc cgggtggaga gaatgcactg attcgtttta aatctgtcgc ggaggaagaa
     3661 gttgagtcgt ctcgttaata ttgtggctgg aatgctgccg tttcttgcat tgttgcccct
     3721 cagtggttgc gccagccgtc cggattacgc cctgagtcca tcgggtaata cggagtgggt
     3781 gacagtggcg gtgaaactgc ctccggagac ggaggctctg ccagtggatg tactgtatcg
     3841 ctcggaaatt tgcaggcgta aggattatga ccctacgacc gaatcacatg ttcgtaccat
     3901 tcggggattt aatccggtac agatcccact tagccccccg gataatagcg gttttcgtaa
     3961 ggtgcgaatt gctctggatg ggggtacacg atgtggctgg actctcagcg gtgtccgggt
     4021 ggggattcag atgtccagaa gctcagctct ggcgcagggt aaagatgtta cagcagcaaa
     4081 ctatgtcttt gattttgatg atgagggata tggaagcgca ttcggtaaag ggaagcctct
     4141 ctctgtgagc ggtgatttgt tttttaaaac tgaatttttc ccccttgtat atattaatca
     4201 tatgtttaat gaaacatatc ttgaactatt tgctggcaat gcaaaatatg aaaaatggaa
     4261 tcgatattat agggttcgtg agactgagaa tattgcaata gaacctgtgc tatatgtaaa
     4321 taaagttgtt agggttgaga gtgctaaagg taaagagaat ggtccgggga tgattgtttc
     4381 ttatccagat gcttcaacgg aaaagattaa ggatatttat cctgattaca ccaagttact
     4441 ttcgatgaag taaaggatct tcttgagatc ctttttatct gcgcgtaatc tcttgccttg
     4501 taaacgaaaa aaccacttgg ggaggcggtt tagtcgaaga ttaagtcagt tggggaactg
     4561 cttaaccggg taactggctt aagtggagca cagataccaa aagctgtcct tctagtgtag
     4621 tcgttataag gcttcaactt caagagttct gcgtatccaa atccactatc ccgttaccaa
     4681 tggctgctgc cagtggcgtt aagtcgtgtc ttacggggtt tgaagcccaa ccgtacgaaa
     4741 acgtactata agggcttccc cggcatgtca acgccagttt ttatgttttt gcctgcttgt
     4801 cattaaaaat aaagggaaga gactgcggta ctataaacgg tcataatgaa tctctttgcg
     4861 tacaaaaata tccgtttcgt gagctgaccc cagttaccgc atcattctga tcgtagtgtt
     4921 tcaggcgtac tgtccgcgta aaagcgggca gtgtttagtg gcttaactca tgacaacctg
     4981 ctgtataatt tgcgttttca ccactgatat ctaacagtgc ctggcgaaag gaggcattga
     5041 acatcggccc gtgtcctagg ttggggaaat cccaaaatgc ggtattgacg cctttatctt
     5101 tcagtatagt tagggtggta tgaattttcg gcagcacacc gacagcatgc gtttcccggt
     5161 tatcacccgg tgtcgccgag ccttccatta tcgccaggtg tttggcgcag aattgcagtg
     5221 gctcaaccgc cgtaacgcgg cttagcagag catcattacc tctgcccaac gatgggctgg
     5281 cgctgtagta caaccggaag taagaagagg agagccaggt atccagcacg aagaggccgc
     5341 cgtaggagtg cccccataag ccgcggcgct gccgatcgat attcaatccc tgttccactt
     5401 ttggggcaat acgcgtttct agtaagtggc ggaagtcgtt gctgccacca ctcttacggc
     5461 taaaatgccc tgagtgggga tctgtttttc tgccttctgc tgctggcgta tagtcgtaag
     5521 ccctgctgtt gagatcgaaa gggaggttgg tctgataccc gacagccacg atcactggcg
     5581 gtgttttttc tgacaattgt ttgagcagtt cgtcatccag gcggtccata actgcgttac
     5641 cgtcaagcat atataaaatc gggtaacccg atgccggtgc ggttttattc ggcacggccg
     5701 tccatacccg ataatggcgt gtgtcatcga cagagtcgaa agaggtgacg ctgaaatggt
     5761 aaaacacgga gcctttatcg gctatattcg gccccagtgg tcgcatatcc ggctttgcat
     5821 aaacttgcgc actacagata agggtaagac aggaaagatg aaagaaaatc gctttacgcg
     5881 ggcgtgttga gcgatactcg ctggcataca tctgatgatt attccttttc cgcaggtgac
     5941 aatttgattt attcaggacg tctgtaccgt ttcctgaaag tgtttgtggg tcaactcaac
     6001 cctgtagtaa accaatcccg tcaatcagtg caccgtgcca ccacgtgtaa tcgtgaccac
     6061 cggtgtagat tgcgcacgat aatttttatc cccatgacat tatccctgca ctggaaatgg
     6121 ttaaataaaa cctatcgtat actaggctga gatagcctta cggggttcag gactgtttat
     6181 taaatttaat ggacgaataa tatgtatgta aaccacagcg tgctaccttc catgcgctgg
     6241 tgcggcatca gagtaaaaaa tcagcattaa atttatttgc aaatgtatga gtaatacaga
     6301 cttgttaacg ggtcctttca gatgtatctt taagacttat taaacagtct taaaaggtgc
     6361 attatgccta acatcattct gtctgacatc agtgcaagtg tcagtgaact gaaaaaaaat
     6421 ccgatggcga cagtcggtgc tggtgatggt tatccggtcg ccattctgaa tcgcaatcag
     6481 ccggcattct actgtgttcc tgctgaactg tatgaaagga tgcttgatgc tctggatgac
     6541 caggagctgg tgaaactggt tactgaacgt agcagtcaac ctctgcatga tgttgacctg
     6601 gacagttatt catgacttat acgataaaat tcagggatga tgcgcttaaa gaatggctga
     6661 aactggataa gaccattcaa caacagtttg tgaaaaaact aaaaaaatgt agcaaaaatc
     6721 ctcatatacc gtctgcaaaa ctacgtgggc taaaggactg ctacaaaatt aaacttcgtg
     6781 catcgggatt tcgtctggtt tatcaggtga ttgatgatat gttaattatt gcggttgttg
     6841 cggtaggtaa acgagaacgt agtaacgttt attatctcgc cagtgagaga atgagatagt
     6901 aacataaata gcctttcccg cagataactc tcagaattgt tattctctct gtggcctcgc
     6961 ctgaggcgga aaaagaaaag ttttgttgag agaatgattt tacctgccgg gatgagtctg
     7021 gggttattta tatcaccaga caaattgaaa cggagggaca tgtttgatga caatacttgt
     7081 gacggcacag gctcaactca tagttggtga ggctatggcg ataaaaagtt tagctcctga
     7141 tggaatgttg gccgctgtat ttgaagacga cgggaatacc ggatatttct atgcacttga
     7201 tgagtccgtt gaaggtaacc ctgtcaggga tgctgtgcat atatataacg ttgaagacat
     7261 ttcagatgct cacatcccct ctgatgtgaa gattggctgg tctgaggatt gcctgaagtg
     7321 tgttctgctg ataaacggat atccacatgg ggtattcgat tttgagggga agaacggcta
     7381 ctgtcgaagt ggattcccgc caccggttaa tcatgaatgg tctgttcagg ggcatgcatg
     7441 ggatgattac gtcgataatt tcttccggtg actgaaccga aaaattacct gacggacaag
     7501 tctggggccg aatcgtgatg attcatcatg gccgtttata gttcagcagt cttgttcttt
     7561 tgctgtcagt gggagaccag gaaaataacc gattgttacc agacagaata accatcataa
     7621 gacgtcacaa gacggtgacg cttacatagt tccggggagt taaacaatat accctgactt
     7681 tgtaatcatt tgaattatat ccgtaacgtg taacttcctg ccgtaacacc ggatttaaga
     7741 tattcaaact ttacctttat accgatatta agagacacaa tgcgaaaaat aattgtccat
     7801 ttcaagatag ttttaacctt acttctgccc gcaacagtat ctgcccagtc gatagagtgg
     7861 caatcctgta tgactactcc attcagtaac tggtttggtg aggaaaaacc gtctcctgac
     7921 ttactatgtg gttatttgtc tgttccatta aaatatacag acacaggcga agatagttct
     7981 tctgtaaaaa gacagcaggt aaaactggcg ctgacaaaat tgccagcaaa aagcaggcat
     8041 aaagggagta tcattataat aagtggtgga ccaggattac ctggcattaa tccttatatt
     8101 aactttgact ggccggtaac aaatcttcgc gaatcatggg atattattgg ctttgatcca
     8161 cggggagtcg gccagtccat tcctgcaata aactgccagc aatcagatac agggattcag
     8221 gaaaacataa ctgaaaagca acgggtatta aataaaatta acagctgtat ccataatacc
     8281 ggaactgaag ttattcgcca tattggttct aacgaggcgg tatatgatat tgatcgtatc
     8341 aggcaagccc tgggtgacga gcaactgaca gctgtggcat attcgtatgg aacacaaatt
     8401 gcagccttat atgcagaacg tttcccatcc agcatcagat ctatcgttct tgatggggtc
     8461 gtcgatattg atgacctggg ggacaacttc acatggcaac tcaagcaagc acagagttat
     8521 caggaaacgt tcgatcgctt tgcatcctgg tgtgcacgta caaaaagttg cccactttct
     8581 tcagtcagag ataaggcaat aactcagttc catgagctgt tatcaaaatt gcatcacaag
     8641 cctttattag acagcaaggg agaaaatata tcttcagatg aactcatatt attaacaaca
     8701 gaccttttgt tatggcgttc atcatggcca acccttgcaa ctgccatacg tcagttctct
     8761 caggggattg tcagtaatga aattgaaact gcgctcagtg ctcctatagc ctcagaagag
     8821 tcaagtgatg ctctgggggt aatcctttgt gttgatcagg ggggggagca attaacccca
     8881 gaagagcgaa aatcccgaaa agacgctctt gcgaatgcct tcccggctat taactttgac
     8941 aatggacgtt ccgattcacc tgatttttgt gaattatggc caatacatag caacctgaat
     9001 aaaacccggc tgaaaaatac agttctgccc tctggtttac tgtttgtagc acacaaatac
     9061 gatccaacaa caccatggat taatgctcgt aagatggcag agaaattttc cagcccgttg
     9121 ctgacaataa atggtgatgg gcatacatta gctcttaccg gagtcaattt atgtgtagat
     9181 aaggccgttg ttcgtcatct tatcacccct atgaaaatgg gaaatatatc ctgtccagga
     9241 aatgctgaaa tagatataaa atagtttaaa aattgcccgc catttgctcc tgatacagga
     9301 catattttgc attgggagca aaatgcgttc agtgcgatca cggcaccaga aacgtcaaac
     9361 tggatacacg taaaaaatgt cgcgatcgcg caaaatcaag ccattactct cgggagtgct
     9421 taaataatac gcacaatata caacgctctc ttctctacag tctgtttcct gtaatatcct
     9481 gcggttattt tgatttctca gaaatatttt cctgccgggc cttcatactc agccacctct
     9541 gctctttcgc cgccagccag cgcctggaat tttgagcatc acattccgat acttccccct
     9601 ctggttgacc atccaggtta taacggggag ttcctgcaac agaggttctc tgcaggtaga
     9661 tccggcgact ggtaatgcag gatactgcac aattccattc gtccagggtc agcgctgttt
     9721 ccggatgtgc tgccagatat gtagtaatgt cacgggttat gcctgttttc agaggcagaa
     9781 aaccgccccc ttcggcacga agttgaggaa acagcgcttt caccttctcc agacaatcgt
     9841 gagtgaactc actggtccag tgggatggtc ttggaaagcg ttgtcttttc tttttcttct
     9901 tcctggctgg cgcttcgggg atatgagttt cgacggcagg tgaactgatt ccggaggaga
     9961 ctgattttgt cgcctgttca gtgagtgcag gcgcgataac atcttttttt tcggctaagg
    10021 aaggcggtga aaaagagcgc cgttttttca ccacaatgac gggatttttc ttttcttcca
    10081 gatcttttcc cataaatacc tctgcttcag aatttatttt ttaataatat cacaa
//
LOCUS       DAZGTJ010000137         9988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000137 DAZGTJ010000000
VERSION     DAZGTJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.137"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            86..1666
                     /locus_tag="ACHK8E_004625"
     CDS             86..1666
                     /locus_tag="ACHK8E_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP0346793.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGMR"
     gene            1666..2841
                     /locus_tag="ACHK8E_004626"
     CDS             1666..2841
                     /locus_tag="ACHK8E_004626"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP0346794.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATI
                     VAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK"
     gene            2844..3440
                     /locus_tag="ACHK8E_004627"
     CDS             2844..3440
                     /locus_tag="ACHK8E_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP0346795.1"
                     /translation="MNSTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TTEILATLPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3511..4458
                     /locus_tag="ACHK8E_004628"
     CDS             3511..4458
                     /locus_tag="ACHK8E_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0346796.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(4760..6136)
                     /locus_tag="ACHK8E_004629"
     CDS             complement(4760..6136)
                     /locus_tag="ACHK8E_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP0346797.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(6136..6564)
                     /gene="tssE"
                     /locus_tag="ACHK8E_004630"
     CDS             complement(6136..6564)
                     /gene="tssE"
                     /locus_tag="ACHK8E_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0346798.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(6568..7104)
                     /gene="tssJ"
                     /locus_tag="ACHK8E_004631"
     CDS             complement(6568..7104)
                     /gene="tssJ"
                     /locus_tag="ACHK8E_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0346799.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(7085..8164)
                     /gene="tssG"
                     /locus_tag="ACHK8E_004632"
     CDS             complement(7085..8164)
                     /gene="tssG"
                     /locus_tag="ACHK8E_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0346800.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(8128..9888)
                     /gene="tssF"
                     /locus_tag="ACHK8E_004633"
     CDS             complement(8128..9888)
                     /gene="tssF"
                     /locus_tag="ACHK8E_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0346801.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         2484 a         2380 c         2520 g         2604 t
ORIGIN      
        1 attttagaag aaaaactata tctctctatt cgtaccaaag tattaatgga ctaattatat
       61 ataacattta tatgagataa cccacatgaa acaaaagaaa gcctggagtt tttttcagag
      121 cctggggaag gcatttatgt atcccattgc tctgctaagt gtatgtggca tgatgctagg
      181 gctgggaagt ggtttagcca gtgatgatat ggcaaagtta attccatttc tggctattcc
      241 aataattaaa accatacttg atttcattgt tagtcttggt ttgtttgcct ttgttaattt
      301 acctgtattg tttgcgatag cgattccctt aggattatta aaagataaag aggataaagc
      361 ctatggtgct ttttctggct taattggttt tatggcgatg catctgggaa cgaactttta
      421 tcttaaacag cacgacttat tggtcgttgc tgaccaaatg tcgacacatg ggcaaaacat
      481 cattctgggg atccagtcct acaataccag cgtgttaggg ggaattgttg ctgggttatt
      541 agtcgccagc atgtataaaa agatcgttaa tttacgcatt ccagaatcat taggttttta
      601 tagcggccca cgtctggtgc ctatcattac actgattgtg atgagtggat ttggtctgat
      661 cattcctttt atctggccgc catttttcaa tctcttcatg ctcattggcc actggatatc
      721 aacttccggt cctgttggtt atttcttcta tgcagttgcc gaacgtgtga cgattccttt
      781 tggcttaaac catctggtga cgtcagtgtt ccgctttacg ccaatcggcg gttcggctgt
      841 gattggcggc gaagagtatt acggcaccct gaacatgttt atggcgtacg tcaaagagaa
      901 tgcggtcatt ccgctggatt tggcggggaa aatggagcag ggcaaactga tgattcagta
      961 cggtctggct ggtgctgcac tggccatgta tcgcactgct catgctcaaa acagaaaggc
     1021 tatcaaagca ttgcttattt ccggtgtgct tacggttatt attggtggcg tcagcgaacc
     1081 gattgaattt ctgttcttat ttgtcagtcc gctgctgttt gtcttccatg cctttatgaa
     1141 tggattcgcc aacatggtct tgccatatat gggcgtgaag atgggattta ctggcgatct
     1201 gattcaattt attagctttg gcgtattgcg tggcacaaga acaggttggc cgatcgcggt
     1261 gtgtgttgaa gtggcctatt tcttcattta ttactttgtg ttccgttgga ccattctcaa
     1321 atttaacctg atgaccgtag gacgtgaaga gtccagtcct gtcacgctga acgctcacga
     1381 agatacggct atagcggata ccccaactct tgataaatca gagttgcaag cagcggagca
     1441 gatggttaag gcacttggtg gtaaagagaa tattaagtca ctggataatt gcgtaactcg
     1501 tttacgttta acaatcgcag atatgggatt gattgatgaa gttgcaataa aaagagctgg
     1561 cgggattgcg gttgttaaac ttgatcaaaa taccctacaa gtcattatcg gcactaaagt
     1621 catcgccctg cgtagggata tggataacta catggggatg cgctgatgga tatttttaat
     1681 actcctattt cccgtaaagg aacatattgc acccaatggg acttttgcga ggacagattt
     1741 ggtgtaaaag atgtattacc cttctctatt tcagatatgg atcttccgat accagacgct
     1801 atcaccagag cattaaaaaa acgcctggaa cacccgattt tagggtacag tcgttggcaa
     1861 catggtgaat atctgaatgc gatagtaaac tggtatcatc aacaatatca aacagacatt
     1921 aaacctgaat ggataaccta tagccccagt gtgatgtact ctattgcaaa ggcaatagag
     1981 ttattgacat cgcatggtga taatattctc gtttttacac ctgtctataa tgcatttttt
     2041 gatgtaatta aacatagcga gcgaaatatt ctgacagcct ctttgattat aaaaaatgag
     2101 gggggctacg ctatcaactg gcaagatttt gatcttaaga ttaaaagcgc aaagatggtg
     2161 ttattatgta atcctcataa tccgacagga acagtctggt cggaagaaga actgcttaaa
     2221 atcgctacaa gctgtacaag gcataatgtc tggttatgtt cagatgaaat tcatagtgat
     2281 tttgttttta atcgtagctt tacatccgcg cttaaaataa aaaaagaaaa agtagtggta
     2341 tttaactcga tatcgaaaac atttaatgtt cctgcgctaa caggatcgta tctgatttca
     2401 actgacgagg attataatca taagttcaga acgatatcga ggtatcgcga tttcgtaaac
     2461 tccccatcag tactgaatat aattgcaacc attgttgcct ataatgaatg tgagggatgg
     2521 ttaaagtcat taaaggcaca tatcgcctct aacattcaat tcacccagca gtatctgaac
     2581 gaaaatactc cagaattgat cgtaaggcca gctgatggtt gctatttttc gtggatagat
     2641 tgctctgcta ttggttattc ctttgatgaa ttctacagtc gattaattca tgaaggcaaa
     2701 gtgggcatta tggcggggaa tgtctatgga acggaggggg agggctacct tcgtctgaac
     2761 ctggcctgcg gtcgagaaaa attatatatg gggcttacgc gtcttgcgag tgtaattaaa
     2821 aatattaatc aaggaaaata aaaatgaaca gtacggatct tatccatctc attaaacatt
     2881 ttatgcgtaa cgaacataaa gcagttgaag aagttattga ttcccccctt tcggaggtcg
     2941 caaatttaat taaagtatta cagtcttgtc aggggaaagt tgtttttatt ggtgtcggta
     3001 agtccggtat tattgcccga aaactcgccg caacatttgc cagcactgga actccctcgt
     3061 tttttgttca cggtactgaa gccgtacacg gcgatcttgg gatggtggcg aaagatgatg
     3121 ttgttattct tatttcaaac agtggtgaga cgacagaaat cctggcaaca ctacctagtc
     3181 tgaaaaaaat gggtaattat ttgatctcct ttacccgcag tcatcgctca tcgcttgcga
     3241 taagttgtga tttatccgtt gaaattcccg taaaaagtga ggcggataat ttaggtttag
     3301 cgccgtcatg ctcatcaaca gtcgttctgg ttgttggtga tgcggtggcg cttgcgctct
     3361 ccgaattgaa aaagtttact cgtgctgatt tcggcttata tcatccagga ggtgcgctcg
     3421 gtattaaagc aaactcataa ttttagaata taaaacagta accggtcacg ccatcttttg
     3481 gggtggcgtt cattgatgtg agtgtcagct atgaaaaacg ttctggtcac cgttcccagc
     3541 ttttcagcgc gttgtgtttc tgccagcaag ctgctaagag aaaatcattt taaccttatt
     3601 atcaagaata atgtggagca tctggttaaa tcagaatcca cagtattacg agaatctatt
     3661 tgcgcagtca ttgccggaaa agattgttat caagcggata cgttatcgcg actaccagga
     3721 gtgaaaataa tatcgagatt cgggacaggg attgataata ttgatcttca tacggctcga
     3781 caatcaggta ttgtggtcaa taatgctgtt gggattaatt caaatgcagt cgcggaattt
     3841 atcatcggtc ttatttttgc gagcatgaga aatatccccg gtagctatca tgcaatgcaa
     3901 aatggttatt ggggtgagtc gcatggctgc gagttacaag gtaaacgtat tggcctgctt
     3961 ggctacggca atattggcaa aacactggcg aaaaggttat ctggttttga tgttgagctt
     4021 ttagcttttg acaaacaacc cgactatcag gttgctgaca aagctggagt tcagtttgta
     4081 tcaattgaag atatctttat gcagtcgcat gtcatcattg ttcttttgcc tttttcttct
     4141 gagctggaga acttcattag tcataaatat ttgtcgatga tgcgcaatgg tgctctgatc
     4201 atcaatgccg caaggggaaa gttgctggat gagggcgctt tacttcaggt gattgaagaa
     4261 cgaaatgtgt ttgcggcgct ggatgtgttt agctctgaac cgttggcgca atttagccca
     4321 ctgctacatg ccaaaaatat cattacaacg ccgcatatcg cggccgccac cgttgagtca
     4381 taccagcaaa ccgggataca cgttgcccag tcgataattg attacttcgc aggaagggag
     4441 ataaaaaacg tgttgtgagt tcagcgaaca aagattttaa atacaaaaaa accgcaattt
     4501 gtagaacaca ttgcggtttg tcagcattct aataaattgg ttgcgggggc cacagtcaag
     4561 aggttaatcc aatcttttga aacttaaggt attcactgtt cccgaaccca agctatctac
     4621 cgaaatatac actcattatc tgatgtgaat caacgagtag atcgaatggc ctgttcctct
     4681 ctttttttgt acacagagac tccattttaa aaaatggcaa ggtcacatat cccttattgg
     4741 tacataaatc ccccgatgtt tactgacttt gttccacctt cgtatccagt aaaatgacat
     4801 agcgattcaa caactgattt aaccgggtat caatttgcgt caacagcgtt ggcgaaacct
     4861 ctccggtctg tttttgctct tccagttgcc gaagcagttc ttccagcggc ggctccttta
     4921 acgactgccg gatgccgtac accgccgttt ttagttccga accggtcaga tatctcccct
     4981 tacgttcatc cagtgcatcc agctgcgccg acaggcgctg tagctggctc tgcgcctgag
     5041 catagccatt cagtgcctca gcagacaggg cgctcagctc ccgctgtttg cgccactgcg
     5101 cgttcagcgc cctgacctga ggattatccg gccacagggc atcaagctgg cgtagctggc
     5161 gatatccctg ctccagtggc cagcgggcag gcaatttgtt taactgctcc agttgcatct
     5221 gacttgccgc aattgttctg tcctgagcca gcagcgcctt atcctttaca tccagctttg
     5281 ccagttcgcc gctctccagc gactgcggca acggcatgac agacagttgt attcgctgga
     5341 ttagcgcgtc cggctgatta agctgcgacc agccccataa tcctgttccg ccgagaccgg
     5401 ccatcacaac cattccggcc ataaatatcg gccatgtccg ctgccgggag atttcagggg
     5461 atggcacggg tggactggca tccttatcgt tttgctgaac ggatctggtt gcaaaaaccg
     5521 gctgtggact ttcctctgac tttttcggtt gattcatttc ccggcggggt aattcagggg
     5581 gagtgataat ggtttccgct ccctgaggcg ccagccgttc cagacgcaga gccgcattgc
     5641 gaaactgccc ggacaatacg tccagcttcg tcatatgcca cagttcacac tgttgcagcg
     5701 tggtttcgat ggcggaaaga tgctgtacac aacggtagat ctgcggcaga tcctgatact
     5761 gaatatccag cgttctcagt gcctgctgca ttttttctgt gacccagctg agcagtgata
     5821 ttctggaatg tacctgtacc ggccagaagt cagcccagcg ctggagcagt atatccagtg
     5881 aaccgagtcc gtctgccatc cctgccagcc cctgccgtct ggttattgcc agcacgtaac
     5941 agaccagcgt ctgtagttcc acgccatttt gccggaacag cgcgaggcag agtttttcca
     6001 cctgacgcca gtccacatcc ggtcgggccg ggtgagtcag tttactcatt tcagcctgca
     6061 gttcaccgga ttccttataa gtacagggat cgccgcccat gacgaactgg ctgataaaat
     6121 tcgcgttact tgccatcagt acgcccgctc ctgttgtttg agatgacgca gaacaattcg
     6181 tccttccgga gaaaatacgc catcaaagcg tatccagccg gtatcgggaa gctgcgcgtg
     6241 gatcaggtaa ttaagatgcc ccggaccggg ctggggcagt aactccacct gtatctgatt
     6301 caggcgtggt tcataacgca gcagcgtttc ttcaatctgg cgcatcaggc tatgaatacc
     6361 cgcagccatc ccctgatgga tcaggctgag atccggcaga ccgtaatccg gcaaatgcgc
     6421 cagtgtgcct gcccgacagt tgataatgcg ttgcatattg tccatcacgg aaaggataag
     6481 ctgatcccgc tcactgacct gttccagcgg taactcgccg ctgaaactga acgtcagcat
     6541 ttcatataag gaaggtgtcg ccatcggtta tttatccttg agaggcagca gtcgcaggtc
     6601 gcttctcatc agttcaatcg agcgcggctt gtcggcctcc agttcgtccc ttttgatcac
     6661 cagacgccag ctgtcgcttt tttcatccgg gtgataaaac tgcccaataa tggcgacaaa
     6721 ctgcgtctct ttatccagcg gcacattcag ggatgccgtg ccttcaggtt ttacccagac
     6781 ggtgtgtttt gccagtacgt ccttctccag cgtcttctct tcctgcataa acagggtgtc
     6841 ataatccgcc ttatcaaaat cttcgcgggt tttcagttgc cagacatgca ccatcgtgga
     6901 aagcggcgta tcttccgcat ccgtattcag ggcgctgcgg gagaagaaat cgagatgcag
     6961 tattttgatt tgtttgtaga aaacggctga ggccacgctt ttcgattcat ccaccacttt
     7021 ctgaatcagc ccgcagccac tcagaacaac cgtcagcatc gccatacaga gcaacgccgg
     7081 acgtttaaaa cgaataggaa acatgagtca tactctccct gttacgaatg gaaaaagcgg
     7141 gtattaatcc ctgataacgc cccagactga ccgtcactgt tccggtcgcg ggcgcggcaa
     7201 aggaggcgcg aaggatgccg gtacggctaa tctgtacacg ctgttttccc agccgggcag
     7261 caggcaacag gctgacgggc agcgtcagtt gcagacgggc ctgacagcgc cagcccagat
     7321 aaacgcgcag cagcaccagc aaatcgctgt gcagttgccc gccgggcagc cagccacgtg
     7381 cttctgcggc atcttcggta tacagtttca gcagcaactg gctgttgacg tcggtaccgg
     7441 tacgccccag cagcgttcgc gttttcaggc tgatgcgtga attttttgac agacccgcag
     7501 gggccgggag aattacaggc tgcggatcgt gaggcaccac cgtcgctttg gtcagcgggg
     7561 caagcagccc taccagtgcc gtgacgcctt ccgccgtgcg cgtcggcagg cgcatcacgc
     7621 tgagcagggc cagaaaacgg gataccggcg tggcgatatg gttctcgctg ccgggaatac
     7681 ccagcccgat gagtcccagc agacaacggg aaatgtcatc cacggcacct gcttcaaacg
     7741 acgccgggta gttatatttg cgccagatgc ggtagtactg agtaataaag cgatggttaa
     7801 aaatatcgag aaacgccatc actgcgtcat gtccgtcatg acgctgggta atgtagtcca
     7861 gataggcggt tggcagcggc gaatccacgc cgtacaggcc gagaaaagtg gtgcgcaccg
     7921 tggggggcgc gtcgggattg cccacgtcac gttcaacgtt ttttaactcg ctgaccggaa
     7981 agcccatacc tggatgagga cgaaagcgca ccgcatcggt ggcggggctg tcggtactgc
     8041 ccagcggcgc gttctgctgg ctctgttcca gcaactggca aaaacggtaa aaattgatcc
     8101 gcgcaatatc cggggccagc cggtcggtca gcccggtacg cgctggctgt gatgctctgt
     8161 ccattgcagg cactttcctg tgggttgcag aattaaggtc agttgagtga ataagtggat
     8221 atcggcatac agtgcaaaga agcggtgcag cagttcgcca aacagcgtga tatcaccctc
     8281 tccggcaaag ccggtgctgt ccagcgtcac ctgaatatcc acgccacgca gcagaaagcc
     8341 tctttcaaaa cgctgtatca ggctgtgctg aacgtcgaca atcgccgaca gacggcggcg
     8401 gttcatctcg ctttcggtcc agtcgtacag cgccagcgtg ccgcgcagaa tttcggcgtt
     8461 atccatcatt gaaagaaagt tagaaccgag gtggctgagc acccgccagt ggaagcggtc
     8521 acgcgccggg ggataacagg gctgcgtcgg ggcgcacagg ttgcgcaccc gcaggacgtt
     8581 ctgtgaggca tgaactggcg tatcaagcag cgtgctctgt aaagccttac ggggaagctg
     8641 tccgttagtg ccggtcaggc ttaaagagag cgtttcatcc tccagcatcc ggtcggaatc
     8701 aaacgcatcg ccgcccagaa tcagccaggt atcatgcagg ccggaagggc cgcgcttaac
     8761 ccgcgtatgg taatagcgtt ccggtgcatc gtggcgcagc atcccgcccc ggtgacgaaa
     8821 gctggaaaac gggacatacg cctgactgcc ggtatggcgc gaagaaataa tgttgtcgac
     8881 ggaataaatc tcggtatgcc cgtcctgaat acgcatcggg cgcagcagaa attcgttttc
     8941 cagtggcgaa aggtgtaacg gatcggcttc cagcgggaac aggttgatga ccggcacgca
     9001 gtgcagacgg atattatcgc tgtcaaacgg caggtcgtga ggccagttct cgctcagcac
     9061 cacgtcaatc tcaaagccgc tcatcccttc cggccagatg acgttctcca gcccgttcag
     9121 cgccacaaac ataaactttt cgcggaaggt gaaatactcc agcagcaact ggtagccgct
     9181 gaacgccgac tcgccttttg gccacagtcg gtcattgtca tcaaaaccca tcggggaaaa
     9241 gtgcgcgtcc agcacacggc gttcctgccc cggcagacgc agccagaact gctgggtgcc
     9301 aagcgttaaa gcccggtgca gcgcacaggc caccgggctg tcggcattga gatagagcgg
     9361 caaacgggat agatcgatct gtgaccagtc accaaccagc gggccgcatt caaagcgcag
     9421 acggataacc gagcgcccgt ccggttcata ttgcaatgac acgtccggca gcgccagcgg
     9481 atgcagcgtg atatcccgcg tagcactgta gacacagcgg gttctgcgct cgccaacagg
     9541 acgcgacaat acctgaaaat tactcagtgt ttcgctctgc ttcatctgcc ggtgatctgt
     9601 cgacagttca accacggaaa gtgacgggat ggttcgcagg tagtgaggcc acagcaggct
     9661 gaccagcccc tcggtcagtt cgggcaggtc gtcatccagc ttttcacgca ggcgacccat
     9721 taaaaaagcg aagccttcga acaggcgctc cacataggga tcgcgtgcgc cgggtttatc
     9781 aagattgagc atcgcggcgc ggtcggggta agcctgcgca aattctttcc ccgcttcacg
     9841 caggtagcgc atttcggctt cgtagtagcg ttgggttaga tcgtccatcg gagggattat
     9901 cttcctttca ttgaaagtga atatcatgca ggtgtagaag taacatcata gttaaattta
     9961 ttctatgtga atatattatg gatagttg
//
LOCUS       DAZGTJ010000138         9951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000138 DAZGTJ010000000
VERSION     DAZGTJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.138"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(172..1332)
                     /locus_tag="ACHK8E_004634"
     CDS             complement(172..1332)
                     /locus_tag="ACHK8E_004634"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0346802.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1338..2009)
                     /locus_tag="ACHK8E_004635"
     CDS             complement(1338..2009)
                     /locus_tag="ACHK8E_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP0346803.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2157..3638)
                     /gene="tolC"
                     /locus_tag="ACHK8E_004636"
     CDS             complement(2157..3638)
                     /gene="tolC"
                     /locus_tag="ACHK8E_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP0346804.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3843..4472
                     /gene="nudF"
                     /locus_tag="ACHK8E_004637"
     CDS             3843..4472
                     /gene="nudF"
                     /locus_tag="ACHK8E_004637"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP0346805.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4473..4895
                     /locus_tag="ACHK8E_004638"
     CDS             4473..4895
                     /locus_tag="ACHK8E_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP0346806.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4920..5747
                     /gene="cpdA"
                     /locus_tag="ACHK8E_004639"
     CDS             4920..5747
                     /gene="cpdA"
                     /locus_tag="ACHK8E_004639"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP0346807.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5747..6328
                     /gene="yqiA"
                     /locus_tag="ACHK8E_004640"
     CDS             5747..6328
                     /gene="yqiA"
                     /locus_tag="ACHK8E_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP0346808.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6357..8249
                     /gene="parE"
                     /locus_tag="ACHK8E_004641"
     CDS             6357..8249
                     /gene="parE"
                     /locus_tag="ACHK8E_004641"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP0346809.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8294..9163)
                     /locus_tag="ACHK8E_004642"
     CDS             complement(8294..9163)
                     /locus_tag="ACHK8E_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFP0346810.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9177..>9951)
                     /locus_tag="ACHK8E_004643"
     CDS             complement(9177..>9951)
                     /locus_tag="ACHK8E_004643"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP0346811.1"
                     /translation="RSPKIYFILKGNIMNSYLKISLAGITMLSCLSPTLVCAEQPHGF
                     IDYRHEYLDDTRTHADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTGL
                     SVVYRFKPLDDKKFWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKA
                     TDKSKGYGNDDRNNRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHKNYTASFK
                     VGYKMGSWIPYMSIADYKGVDKTSSNRQIRWRWGLTYTF"
BASE COUNT         2391 a         2394 c         2580 g         2586 t
ORIGIN      
        1 gatgtattca cttactttaa ttacaatatc gcttgcatat gacgatttaa cattaaattg
       61 agtattaatg ttaaataaag gttggatatg tatgtgttta tatattgatg ggaattatga
      121 atggtggatt ataagagatt gccccgctta tcggggcaat attaagctgt attactcaac
      181 aaaaatatgc ggataaaacc gagacatatc ctgggtgatc aatgcacggt cttcacgaat
      241 gccaattccg gcaggttgat cgttcaccag ccagctacca atcagcatat agctgtcgcc
      301 gaatttcggt aacgggtgga attgctgaac aatcatccct tcttcgccat acggaccttc
      361 cgctgcttca atggttttgc cgttctcaat gatcgacacg tttgcgcctt cacgggagaa
      421 gatcggttta accacatatt tttccatttg cggatgatca tcttccgcaa aataagcagg
      481 cagcaggttc gggtgattcg ggaacatctc ccacagtagc ggcagaagcg ctttgttgga
      541 gataatgctc ttccacgccg gttccagcca gcgtacgcct gcatcctcca gcttggtgga
      601 gaacatctcg cgcagcataa attcccacgg atacagcttg aacaggttgg aaattacctg
      661 atcctgtaaa tccgtgaact gacctttttc gcctaaaccg atatcgtcga tgtagaggaa
      721 ttcggtagcg atttcagctt ccgtcgcgca gtcctgcaaa tactgaatgg ttccgcgatc
      781 ttccaccgtg tcgcgacagc aggtgagatg cagcaactgg aagccatact gttcacgcaa
      841 ctcgacaaag cgatcgatta gcttttcttg cagactgtta aactggtcgc tgccctccgg
      901 caagttaccg gcgttaagct gatcttccag ccagatccac tgaaagaacg ccgcctcgta
      961 tagtgacgtt ggtgtatcgg cgttattttc cagcagttta ggttcgccag tgccatccca
     1021 cgccagatca agacgcgaat aaagcgatgg ctggtgagtc agccatgact gacgcacaaa
     1081 actccaggtg tgttttggaa tgcggaattt ggtcatcagc tcatcgctgg cgatcacttt
     1141 ttccaccact ttcaggcaca tctggtgcag ttcggcggtg acttcttcca gcttttcaac
     1201 ctgggcgagg gtcaacttgt agtaagcatc ttcacaccag tacggctcgc cgtacatggt
     1261 gtgaaaattg aaaccgtatt cgtgggcttt ctcacgccag tccgggcgct cggtaatact
     1321 gactctttcc atcggtatca gccacccatt gaacgagaag aggtgccggt tgcgctacgc
     1381 tgcatagtgc tttgtttggc aacagattca ccaaaaccgc cacgggtaac ggtagtggtg
     1441 gtcgccggtt ttggtgccat ggctgtcttc ggtacggtca tggtgcggcc tggctgggct
     1501 gcgccatagt ttttacccgt cgcgtcggta tatttaccgt aagccgggct ggctgggttt
     1561 ttcgaggaga acagcggctg ctgtgcaaat cccgcaccgc cgcccatcag acgacccatc
     1621 atgtaaccgg ccatcagcgg catccagaaa ctcccgctgg attgctgggc ctgcgcctgg
     1681 ttttccggtg ccatgccagc ctgggctggt gcctgctggc actgaccttc gccaaattca
     1741 gcaatacagt cttcacgagt ggcgtatttc ggcgcagtac gttcggcttc tttcagcgca
     1801 ttgttgtacg cggtggtaca ttcggcgctt ttgcctgggt ttgcagctga acagtcgtca
     1861 gcattttgat agagagacac tgtttcatca ctcttttcac agccagccag cataaaaaca
     1921 gtggcaaccg cgagagcgac tggtgtcaga tggcgtgcgc tccagttttt gcggaacgat
     1981 gcgtggcgta tggattttgt ccgtttcatt tttgtcttcc gggaccagtg gtaaataccc
     2041 atcagaatag aggatggctg gtcgaaattg aagcgagaag ggggaagaat gcggcagata
     2101 acccgtatct ttacgttgcc ttacgttcag acggggccga agccccgtcg tcgtcatcag
     2161 ttacggaaag ggttatgacc gttactggtg gtagtgcgtg cggatgtttg ctgaacgacg
     2221 ggtgccgggc tatcaggcgc ataaccatca gcaatagcat tctgttccgg cgtttgcggg
     2281 gcaacgtttt ccggattagt ggaaaccggt ttgctcagcg cattgttcag tgccagaaga
     2341 tcctgctcgt tcaacgtacc cagagctgat ttaatattca actggttaat caggtagtta
     2401 taacgcgcat tcgccagctc ttgcttggcg ttgtacagcg tggtggtcgc atccaacaca
     2461 tcaacaatgg tacgcgtacc gaccgagtaa cccgcttcca tcgcgtctaa tgagctttga
     2521 gcggaaacta cggcttgttt gtaggcgtta atgctactga tagaagcatt aatgttgttg
     2581 aaggaggaac gtacggtttg cacgacgcta cgatgcgcgc tttccagttg ctcgctggca
     2641 ccaacaaagt tgtactgggc ctgtttcacc tgcgagttaa ccattccgcc ctgataaatc
     2701 ggcagcgaga agctcaggcc aactttgttc tggcccatat tgctgtcgtc atactgggta
     2761 ccagcggcac cacgggtttt cgaaccgcta taagaggtgt cagaaatccc ggtagaagcc
     2821 gttaaatcca gagtcggtaa gtgaccatcc tgcgcctggc gaatttgctc gcgcgccagg
     2881 tcctggctca agcgcgcctg taacagcgac aggttgcgtt tttcggcttc tttcagcagc
     2941 gtgttaaccg gctgtggttt gtcggtttta aagttttcga cattcagcgc cgccagttct
     3001 ggatagtagt taccggtgat ctggcgcagc tgctctaccg cgttatcaag gttattacgt
     3061 gcggtcactt cgttcgccag cacggtatcg tactgtgcgc gggcgttctg cacgtcagtg
     3121 atcgctacca ggcccacgtt aaaacgttgg gtggtttgat ctaattgacg gtagatcgct
     3181 tctttttgcg cctgtgtata ggaaagaacg tcaatagcat tcaacacgtt gaaataagcg
     3241 gtcgcggtgt tgaggatcaa ggtttgttga tcggtctgat acgtgacgtc ctgaatccca
     3301 gctgcttttt cctgcagcgt taacgcacgc catttcgaca tatcaaaaat ggattgagtt
     3361 aactgcaggg acgcactggt cgcgttagag ttgatgccgt tcgcgtcgcg gtagccgttg
     3421 ctataggtgt aatctgcacc taaacctagc tgtggcagta atggactgcg cgcttcatta
     3481 attttttcaa aggcagcatc acgatcggcg gcagacttac gcaattccgg gttactaagg
     3541 cgtgcttgct gataaacttg catcaggttc tcggcctggc tcaacgaact gaaaccagaa
     3601 aggctcaggc cgataagaat ggggagcaat ttcttcattt gcattccttg tggtgaagca
     3661 gtatttagcg cgatcaaact gtaaaattat tgcggattct agcagaagcc gctaccgcaa
     3721 aaggttggcg ttacgtgcca tagggcgtta atgtgcctca atttaacaca tgactgctca
     3781 aacggcagtc aaacgtcgct gaaattcaca tttaattcac tattagtgcc aggacattaa
     3841 caatgcttaa gccagacaac ctgcccgtta catttggcaa aaacgatgta gaaattattg
     3901 cacgagaaac actttatcgc ggcttttttt cattagatct ttatagattt cgtcatcgtc
     3961 tattcaacgg gcaaatgagt catgaggtac ggcgggaaat ttttgagcgc ggtcacgccg
     4021 cagtcttgct accctttgac ccagtgcgtg atgaagttgt gctgattgag cagattcgaa
     4081 ttgccgcgta cgacactagc gaaaccccct ggctactgga gatggttgcc gggatgattg
     4141 aagagggtga aagtgtggaa gatgttgccc gtcgcgaagc gattgaagag gcgggactga
     4201 tagtcaaacg gaccaaaccg gtgttaagtt tcctggcaag cccggggggc accagtgagc
     4261 gttcgtcaat tatggtgggc gaagtggacg ccacgaccgc aagcggtatt cacggtctgg
     4321 ctgatgaaaa cgaagatatt cgcgttcatg tggtaagccg ggaacaggca taccagtggg
     4381 tagaagaggg gaaaatcgac aacgcagcgt cggtcatcgc tttgcaatgg ctgcagctgc
     4441 atcatcaagc gttaaaaaat gagtgggcat aaatgaagcg ttacacacct gactttcctg
     4501 aaatgatgcg cctgtgcgag atgaactttt cacaattgcg ccgtttgtta ccgcgcaatg
     4561 acgcacccgg cgaaactgta agctatcagg tggcaaacgc acaatatcgg ctgacgattg
     4621 tggaatcgac ccgatacact accctggtga caatagaaca gactgcgccc gcgatcagtt
     4681 actggagcct tccgtcaatg acggtgcgtc tgtatcatga cgcgatggtg gctgaagtgt
     4741 gttcaagcca gcagattttt cgcttcaaag cgcggtatga ttatcctaat aaaaagttgc
     4801 atcaacgcga cgaaaagcat caaattaacc agtttttagc ggactggttg cgatactgtt
     4861 tagcacatgg agcgatggcg attccggttt actagcgtcg tgaaacctaa ggacaccatt
     4921 tggaaagcct gttaaccctt cctctggctg gtgaggccag agtcaggatt ttacaaatta
     4981 ccgacactca cctgtttgca caaaagcacg aagccctgtt aggggtaaac acctgggaaa
     5041 gctaccaggc ggtgctggag gcgattcgtc cacaccagca cgaattcgac ctgattgtcg
     5101 cgacaggtga tttagcgcag gatcaatcct ctgcggccta tcagcatttc gctgaaggca
     5161 tcgcaagttt tcgtgcgccc tgcgtctggc tgccgggcaa ccacgatttc cagcccgcga
     5221 tgtacagcgc gttacaggat gcgggtatct ccccggcgaa gcgggtgttt attggtgagc
     5281 aatggcaaat cctgttgctg gatagccagg tgtttggcgt gccgcacggt gagctgagcg
     5341 agtttcagct tgagtggctg gaacgtaaac tggccgatgc gccagaacgc catacgttgc
     5401 tgctgctgca tcatcatccg ctacctgcgg gttgtagttg gcttgatcaa cacagcctgc
     5461 gtaacgcggg cgaactggat accgtgctgg cgaagtttcc gcacgtcaaa tacttgctgt
     5521 gcggtcatat tcatcaggag ctggatctcg actggaacgg tcgccgcctg ctggcaacgc
     5581 cgtcgacctg tgtgcagttt aagccgcact gttccaactt tacgctggac accatcgcgc
     5641 ccggctggcg tactctcgag ttacatgctg atggcacgct gaccaccgag gtgcatcgcc
     5701 tggcggacac acgtttccaa cctgataccg cttcagaagg ctactgatgt ctacgcttct
     5761 ttatttacac ggtttcaaca gctcgccgcg ctctgcaaaa gcgagcttgt taaaaaactg
     5821 gcttgcggaa catcaccctg acgttgagat gatcatcccg cagttgccgc cgtatccttc
     5881 cgatgcggca gaactgctgg aatccattgt cctggaacat ggcggcgatt cgctgggtat
     5941 tgtcggttcg tcactggggg gatattacgc cacctggttg tcgcaatgtt ttatgctgcc
     6001 cgcagtggtg gtaaacccgg cggtacgccc gtttgaactg ctgacggact atctcggtca
     6061 gaacgagaac ccctacaccg ggcagcaata tgtgctagag tcacgccata tttacgatct
     6121 caaagtcatg cagattgacc cgctggaagc gccggatttg atctggctgc tgcaacagac
     6181 gggagatgaa gtgctggatt accgccaggc ggtggcgtac tacgcttcct gccgccagac
     6241 tgtcatagaa ggcggcaatc acgcattcac gggcttcgaa gattatttca acccgatcgt
     6301 cgattttctt ggtctgcacc atctctgacg atcaactcga attgctaact taaaccatga
     6361 cgcaaactta taacgctgat gccattgagg tactcaccgg gcttgagcca gttcgccgcc
     6421 gtccggggat gtataccgat accactcgcc ctaaccattt ggggcaagaa gttattgata
     6481 acagtgtcga tgaagcactg gcgggtcacg caaaacgcgt ggacgtaatc ttacatgctg
     6541 accagtcgtt agaagtgatt gacgatgggc gcgggatgcc ggtggatatt cacccggaag
     6601 agggtgtacc ggcggttgaa ctgattcttt gccgtctgca tgcaggcggt aaattctcta
     6661 acaaaaatta ccagttctct ggcggcctgc atggcgtggg gatttcggtg gttaacgccc
     6721 tgtcgaagcg cgtagaagtt aacgtacgcc gcgatggtca ggtttataac atcgcctttg
     6781 aaaatggcga aaaggtgcag gatttacagg ttgtcggcac ttgcggtaag cgcaataccg
     6841 gtaccagcgt gcacttctgg ccggatgaaa ccttctttga cagcccgcgt ttttctgttt
     6901 cacgcctgac gcatgtgctg aaggccaaag cagtactgtg ccctggtgtt gagatcacct
     6961 ttaaagatga gatcaacaac accgaacagc gctggtgcta tcaggacggt ctgaatgatt
     7021 acctggcgga agcggtaaac ggtttaccga cgctgccaga aaaaccgttt atcggtaatt
     7081 tcgctggcga tactgaagcg gtggactggg cgctactgtg gctaccggaa ggcggtgaac
     7141 tgctgaccga aagctacgtc aacctgatcc caacgatgca gggcggtacc catgttaatg
     7201 gcctgcgtca gggcctgctg gacgcgatgc gtgaattctg tgaataccgc aacattctgc
     7261 cgcgcggtgt aaagctgtcg gcggaagata tctgggatcg ctgcgcctat gtgctgtcag
     7321 taaaaatgca ggatccgcag tttgccgggc agaccaaaga acgtctctct tcgcgtcagt
     7381 gtgcggcatt tgtttctggc gtggtgaaag atgccttcac cttgtggctg aaccagaacg
     7441 ttcaggcagc tgaactgctg gcggagatgg cgatttccag cgcccagcgt cgtatgcgtg
     7501 cggctaaaaa agtggtgcgt aaaaagctaa ccagcggccc ggcgctgcct ggtaaattgg
     7561 ctgactgtac cgcgcaggat cttaaccgta ccgaactatt ccttgtggaa ggtgactccg
     7621 caggcggatc tgccaagcag gcgcgcgatc gcgaatatca ggcgatcatg ccgctgaaag
     7681 gtaagatcct taacacctgg gaagtctctt ccgacgaagt gctggcttcg caggaagtgc
     7741 acgatatttc ggtagcgatc ggtatcgatc ctgacagtga cgatctgagc caacttcgtt
     7801 acggcaagat ctgtatcctg gcggatgctg actccgatgg tctgcacatt gccacgctgc
     7861 tctgcgcttt gtttgtaaaa cacttccgcg cattggtgaa acacggtcac gtttacgttg
     7921 cactgccacc gctctaccgt attgacctcg ggaaagaggt ttattacgcg ctgacggaag
     7981 aagagaaaga gggcgtactt gagcaattaa aacgcaagaa aggcaagcca aacgtccagc
     8041 gttttaaagg tctcggggaa atgaacccga tgcaattgcg cgaaaccacg cttgatccga
     8101 acactcgccg tctggtgcag ttgactatcg atgatgaaga cgatcagcgt actgacgcga
     8161 tgatggatat gctgctggcg aagaaacgct cggaagatcg ccgcaactgg ttgcaagaaa
     8221 aaggcgacat ggcggagatt gaggtctgat aatcttaacc cggcaaacat gtgttttgcc
     8281 gggttgtcgt tgattatttt attgggcaca gtttattacg atctggaaat agtgttgcca
     8341 gcgaaccagc cagagttttc cagtccgtag ccattttttc tgcttgtgca gttgttatta
     8401 ctgcaccttg ttgtttaata agctcatcct ccactctggt atagtgaata atggtaaaat
     8461 ccgtagagga ccatttatcc ttagagaaca tcttcgtcat cgcatgaatg tttgcttgcc
     8521 cgtcatcgat aaaaacaatc gcgtcatatt tgttccccat tttatccagc atataatcga
     8581 gaattacgcc tttatcttgc ccggaagaaa aaaatacccc ctggctgtac agccatgacc
     8641 gtttgagttt ccctttttca aagggtggca aagtatcctc tttcttcctg agtgcggaag
     8701 acgaaaaatc aatgccgttt cgctttaatt cccgcaatgt cggaaagaga gtatccggtg
     8761 cacgggatgt cagagcaaaa gcggtatgct tttgttgcca ctgttttaac attgtcggta
     8821 actgtggctc ggtaagctgc atagggctaa gttcgtaaag catactgatt gtattttcat
     8881 acaggcaagg aactttttgc gagtcatccg gctttaaagg caatttatcg gtttgccatt
     8941 gataccagat atcaccgcca atgcgagtgc cactcgttaa taacgtgtta tcaatgtcaa
     9001 aaaccaataa tatcttatcg gatggataga gagtttcctt ttctgtgata attttactga
     9061 ccgcactata ttcgccagta actatatttt ttacctgtgc gtaagataaa gcaggcagag
     9121 cagagagcat catccagaga atgattttaa atttattatt cattttttat tccagattag
     9181 aaagtataag tcagacccca gcgccagcgg atttggcgat tacttgatgt tttatccaca
     9241 cctttataat ccgcaatact catatagggt atccacgatc ccattttgta ccctacttta
     9301 aatgatgcgg tatagttttt atggccatca ttccaggtta aataaccatt attgtaatag
     9361 accaggttta actgatattg gaagttggtt ttttgtggtc gatagtcgat ccacacatcg
     9421 taacggcggt tattacgatc atcgttgcca taacctttgg atttatcggt ggctttatcc
     9481 atgtcataac gaaaccgacc tgaaactttc cattctttgc tgaagtcata acccgcagaa
     9541 agaccatatt cgtaagttga ccaatattgc gtggtatcaa gccagaacat gggttctaac
     9601 cagaattttt tgtcatccag cggcttaaag cggtaaacaa cagataatcc tgttccgtta
     9661 ttgccaaatt gtgaggggtc ccacttatct ttatcacctt catcggtgtt atagcgtaat
     9721 tctcccatca aaccgatacc attactaaaa aatgtaccaa attcaacgcg gtccgcgtgg
     9781 gtgcgagtat catcaagata ctcatggcga taatcaataa atccatgcgg ctgttctgca
     9841 cagactagtg taggtgacag gcaggataac attgttattc ctgcaagtga tattttaaga
     9901 taagaattca taatattccc tttcagaata aaatatattt ttggtgatcg a
//
LOCUS       DAZGTJ010000139         9865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000139 DAZGTJ010000000
VERSION     DAZGTJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.139"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1498
                     /locus_tag="ACHK8E_004644"
     CDS             <1..1498
                     /locus_tag="ACHK8E_004644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346812.1"
                     /translation="GENNTQVMIKYKTERGEEKNIKVVVDDTGKYEINLNGWDIKDAD
                     GAEVKIVDSAGNVKSGNLYNIARLYVDMNTNKAITLDSAYNIIGSQKEGTDPAKALIM
                     SDDNDWVYIGGGISPHIGDQNPGSDNNIDMAGGDDILSSVGAVLDGANINMGDGDDKL
                     YTQDGFASSGTRNIIMGDGNDVISVDNSFGGKNTISLGEGNNLFIVGNYVNSIAENDI
                     TAGSGDDRIEIGTNLDGKNKIDLGDGDNTIQVGGYITNSHTITGNSGDDIIYVATNID
                     GSGSFNLGEGNNNFIVGGYIQGKNTIEMGSGDDTVSVSTRIADNVKIQLNAGDDSVYA
                     GGLLNKAIVDLGDGDDVVTLSGISDNGKRNNMEELVSTNAMLTGGEGNDTLKINGSFK
                     LLNMKNISGFETIDLGESSENHLDVGIKSDMLDISSSSGVKIFTIMGGAGNTVDLGKA
                     NITSHNSVEQGNYADSWYKGDTVDGYTTYTPVGDKSVELHIQQDILVI"
     gene            1844..2827
                     /locus_tag="ACHK8E_004645"
     CDS             1844..2827
                     /locus_tag="ACHK8E_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFP0346813.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            2899..4047
                     /locus_tag="ACHK8E_004646"
     CDS             2899..4047
                     /locus_tag="ACHK8E_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFP0346814.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            4071..5747
                     /locus_tag="ACHK8E_004647"
     CDS             4071..5747
                     /locus_tag="ACHK8E_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFP0346815.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            5757..6497
                     /gene="kdsB"
                     /locus_tag="ACHK8E_004648"
     CDS             5757..6497
                     /gene="kdsB"
                     /locus_tag="ACHK8E_004648"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0346816.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            6494..8521
                     /locus_tag="ACHK8E_004649"
     CDS             6494..8521
                     /locus_tag="ACHK8E_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFP0346817.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            8556..9755
                     /locus_tag="ACHK8E_004650"
     CDS             8556..9755
                     /locus_tag="ACHK8E_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0346818.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMKTQLNAVYYGIF
                     NFRKITN"
BASE COUNT         2674 a         2125 c         2495 g         2571 t
ORIGIN      
        1 aggtgaaaat aatacgcaag tgatgattaa atataaaaca gaacgtgggg aggaaaaaaa
       61 tataaaagta gttgttgatg acacaggaaa gtatgaaata aatctaaatg gctgggacat
      121 caaggatgca gatggcgctg aagtaaaaat tgttgattct gctggtaacg ttaaatcagg
      181 aaacttatat aatatcgcgc gtttgtatgt ggatatgaat acaaacaaag ctataacgct
      241 ggattcggct tataatataa ttggctctca gaaagaagga accgatcctg ctaaagctct
      301 tatcatgtca gatgataatg actgggtgta tattggtgga gggatatctc cccatatagg
      361 ggatcagaat ccaggctctg acaacaacat tgatatggct ggcggtgatg atattctgtc
      421 atctgttggt gcagttttgg atggagccaa tattaatatg ggtgatggag atgataaact
      481 atatacgcag gatggtttcg cttcttcagg tacccgaaat attattatgg gagacgggaa
      541 tgacgttatt tctgttgata attcatttgg tggaaaaaat accatttctt taggagaggg
      601 aaacaactta tttattgttg gtaactatgt aaatagtatt gctgaaaatg atattacggc
      661 tggtagtggt gatgacagaa tagaaattgg tacaaatctg gatggtaaaa acaaaattga
      721 tcttggggat ggtgacaaca ctattcaggt tggaggatac ataaccaact cacataccat
      781 tactgggaat agtggtgatg atattatcta tgtcgctaca aatatagatg gttctggctc
      841 atttaattta ggtgaaggta ataacaactt tatagtcgga ggatatatcc aggggaaaaa
      901 cacaattgag atgggttccg gtgatgatac ggtttctgtt tccactcgta ttgcagataa
      961 tgttaaaatc caacttaatg ccggggatga cagtgtgtat gcaggtggat tgttaaacaa
     1021 ggcaattgtt gacctcgggg atggggatga tgtcgtcaca ttgagtggaa tatcagataa
     1081 tggtaagcgt aataatatgg aggagttagt atccactaat gcaatgctta caggagggga
     1141 aggaaacgat actctgaaga ttaatggttc tttcaaattg ttaaatatga aaaatatatc
     1201 tgggtttgaa actattgatc ttggggaaag ttcagagaat catctggatg tcggtataaa
     1261 atcggatatg ctggatattt catcgtcatc tggggtgaaa atttttacta tcatgggtgg
     1321 ggcgggaaat acagttgatt taggtaaggc aaatataacg tctcacaatt cagtagaaca
     1381 aggaaattat gctgattcat ggtataaagg agatactgtg gacggataca caacgtatac
     1441 tcctgtaggt gataaatctg ttgaactcca tatccaacag gatatattag taatataatt
     1501 ttaatagcat aaaagtggtc gcatatgtga ccacttttat ttgttggctt tagttatttc
     1561 aggttttatt aagaaagaag ctaataatat acatcatgtt gttatttgtt ggtttaatgt
     1621 taacatgtat gataaaaatc atgtgggacg ggcatgtgcc cctccctttc ctttattatg
     1681 tctgataatg aaatgctaaa attatttttg tttgtagact gtgttatatg ctttatggaa
     1741 aagtagtatt ttgatttatg tgaagatgag ctgaactcga tttatcagag gcaagttgtc
     1801 tttatgttat aagtgtttta accttatcgg aagtataatt tatatgccag aaaaatattc
     1861 acctgatgat cagagcagta ctattgatcc atatttaatt acttctgttc gtcagacctt
     1921 ggaagaacaa ggtgcagcat tacaaaactt gtctaaacaa ctggattccg ggcagtacca
     1981 gtgtgtcctt aatttgataa tgaactgtaa agggcacgtt attctttcgg gaatgggtaa
     2041 atcagggcat gtcggtcgta aaatgtcagc gacgctggcc tctaccggta ccccgagttt
     2101 ctttattcat cctgcagaag cgttccatgg cgatctgggt atgattacgc cctgcgatct
     2161 tttgatcctt atttctgcca gcggtgaaac ggatgaaatc ctcaagctag ttccttcact
     2221 gaaaaatttc ggcaaccgaa ttatcgccat taccaataat ggaaattcca cgctggcgaa
     2281 aaatgctgat gccgtgctgg aactccatat ggcgaatgaa acctgcccga ataatcttgc
     2341 accaacaacg tctaccaccc tgaccatggc gatcggcgat gcgctggcga ttgccatgat
     2401 ccaccaacgc aaatttatgc cgaatgattt tgcgcgctat cacccgggcg gttcattagg
     2461 tcgtcgcctg ctgacccgcg ttgctgatgt catgcagcat gatgttcctg cggtacagct
     2521 ggatgcgtca tttaaaaccg tgattcaacg tatcaccagc ggatgccagg gaatggtgat
     2581 ggtagaagac gcagaaggtg ggctggcggg cattataacc gacggtgacc tgcgtcgctt
     2641 tatggaaaaa gaggattctc tgacatccgc tacggctgcg cagatgatga cacgtgaacc
     2701 gctgacgcta ccggaagaca ccatgatcat tgaagcggaa gaaaaaatgc aaaagcaccg
     2761 cgtctcaacg ttattggtga ccaacaaggc aaataaagtc actggccttg tgcgcatttt
     2821 cgactaatta agcaacgggg tttgagaggt tagtctctca ttgagtttgt gccccactgg
     2881 aactttgata atcgttacat gttgataaaa gtgaagtctg ccgtatcctg gatgcgtgct
     2941 catctgtctg ccatctcact gtcagatatc caaaaacacc tggtgaaaat catcattctg
     3001 gcaccgatgg cggtgctgct gatctatctg gctatcttca gccagcctcg ctatatgagc
     3061 gagtcgaaag tcgccattaa acgctcggat gatttaaaca gcggcagcct gaattttggt
     3121 ctgcttctgg gtgcctctaa ccccagttcc gcagaagatg cgttgtatct gaaagagtac
     3181 atcaactcgc cggatatgct ggcggcgctg gataaacaac taaattttcg tgaagcgttc
     3241 agccacagcg ggctcgattt tcttaatcat cttagcaagg atgaaaccgc agaaggcttc
     3301 ctgaagtact acaaggaccg tatcaacgtc tcgtatgacg ataaaaccgg attactgaat
     3361 attcagacgc agggctttag cccggagttt gcgcttaagt ttaaccagac cgtgctgaaa
     3421 gagtcagagc gctttatcaa tgagatgtca catcgcatcg cgcgtgacca gcttgccttt
     3481 gcagaaacgg agatggaaaa ggcacgccag cgtctggacg ccagcaaagc ggaattgctc
     3541 tcttatcagg acaacaacaa cgttctggat ccacaggcac aggcacaggc ggcgagcacg
     3601 ttagtcaata cgctgatggg ccagaagatc cagatggaag cggatctgcg taacttgctg
     3661 acgtatctgc gtgaggacgc cccgcaagtg gtgagtgcgc gtaatgcgat tcagtcatta
     3721 caggcacaaa tcgatgaaga aaaaagcaaa atcactgcac cacagggtga caagctaaac
     3781 cgtatggcgg tggattttga agaaatcaaa tcaaaagttg agttcaacac cgagctctac
     3841 aaactgaccc tgacctccat tgaaaagacc cgtgtagaag cagctcgtaa gctcaaggtg
     3901 ctgtcagtga tcagttcgcc acagttgccg caggaatcgt cttttccaaa tatcccttat
     3961 ttgatcgcct gctggttact ggtgtgctgc ctgctgttcg gcaccctgaa actgttgctg
     4021 gctgtcattg aagatcaccg agactaacgc tgtcgctgaa tgagtttgtg atgaaattat
     4081 ttaaatcaat tttactgatt gccgcctgtc acgcggcgca ggccagcgcg gccattgata
     4141 ttagcgctga cccaaacctg acaggagccg cgccgcttac cggtattctg aacggacaga
     4201 agtcggatac gcaaaacatg agcggcttcg acaatacccc accgcccgca ccgccggtgg
     4261 taatgagccg tatgtttggt gctcaacttt tcaacggcac cagcgcggat agcggtgcga
     4321 cggtaggatt caaccctgac tatattctga atccgggtga tagcattcag gttcgcttgt
     4381 ggggtgcgtt cacctttgat ggtgcgttac aggttgatcc caaaggtaat attttcctgc
     4441 cgaacgttgg tccggtgaaa gttgctggcg tcagtaatag tcagctaaat gccctggtca
     4501 catccaaagt gaaggaagta taccagtcca acgtcaacgt ctacgcctcc ttattacagg
     4561 cgcagccagt aaaagtgtac gtgaccggat ttgtgcgtaa tcctggtctg tatggcggtg
     4621 tgacgtctga ttcgttactc aattatctga tcaaggctgg cggcgttgat ccagagcgcg
     4681 gaagttacgt tgatattgtg gtcaagcgcg gtaaccgcgt gcgctccaac gtcaacctgt
     4741 acgacttcct gctgaacggc aaactggggc tttcgcagtt cgccgatggt gacaccatca
     4801 tcgtcgggcc gcgtcagcat actttcagcg ttcagggcga tgtctttaac agctacgact
     4861 ttgagttccg cgaaagcagc attcccgtaa cggaagcttt aagctgggcg cgccctaagc
     4921 ctggcgcgac tcacattacg attatgcgta aacaggggct gcaaaaacgc agcgaatact
     4981 atccgatcag ttctgcgcca ggtcgtatgt tgcaaaatgg cgatacctta atcgtgagca
     5041 ctgaccgcta tgccggtacc attcaggttc gggttgaagg cgcgcactcc ggtgaacatg
     5101 ccatggtact gccttatggt tccactatgc gtgcggttct ggaaaaagtc cgcccgaaca
     5161 gcatgtcgca gatgaacgcg gttcagcttt atcgcccatc agtggctcag cgtcagaaag
     5221 agatgctgaa tctctcgctg caaaaactgg aggaagcatc actttctgcc cagtcctcca
     5281 ccaaagaaga agccagcctg cgaatgcagg aagcacaact gatcagccgc tttgtggcga
     5341 aagcacgcac cgtggttccg aaaggtgaag tgatcctcaa cgaatccaat attgattctg
     5401 ttctgcttga agatggcgac gtcatcaata ttccggagaa aacatcgctg gttatggttc
     5461 atggcgaagt gctgttcccg aacgcggtga gctggcagaa gggtatgacc accgaggatt
     5521 acatcgagaa atgtggtggc ctgacgcaaa aatcgggtaa cgccagaatt atcgtcattc
     5581 gtcagaacgg tgctgccgtc aacgcggaag acgtggattc actcaagccg ggcgatgaga
     5641 ttatggttct gccgaaatat gaatcgaaaa atattgaagt tacccgtggt atttccacaa
     5701 tcctctatca gctggcggtg ggtgcaaaag tgattctgtc tttgtaagga gttgagatga
     5761 gcaaagcagt tattgtcatc ccggctcgct atggctcctc gcgcctgccg ggtaagccac
     5821 tgctcgatat tgttggtaaa ccgatgatcc agcatgttta cgagcgtgcg ttacaggtgg
     5881 cgggcgtcgc agaagtttgg gtggcaacag acgatcagcg tgttgaaaag gccgtgcagg
     5941 catttggcgg gaaagccatc atgacgcgca acgatcatga atccggcacc gatcggctgg
     6001 tcgaggtgat gcataaagtc gaggcagata tctacattaa cctgcagggc gacgaaccaa
     6061 tgattcgccc gcgggatgta gaaacgctgt tacaaggaat gcgtgatgac cccgcgttgc
     6121 cagtggcaac gctatgccac gcgatttctg ccgaagaagc ggctgagcca agcacggtaa
     6181 aagtggtggt aaatacccac caggatgcgc tttatttcag ccgttcgccg attccgtatc
     6241 cgcgtaatgc tgaaaaagcg cgctacctga aacacgttgg tatttacgct tatcgtcgcg
     6301 atgtgctgca aaactacagc cagttaccgg agtccatgcc agagcaggca gaatcactgg
     6361 agcagctgcg gttaatgagc gcggggatca acatccgcac atttgaggtt gccgcaaccg
     6421 gtccgggcgt cgacacccca gcatgcctgg aaaaagtgcg cgccctgatg gcacaggaac
     6481 tggctgaaaa cgcatgattg gcattttctc gtccggtatc tggcgtattc cgcatctgga
     6541 gaaatttctg gcgcaaccgt gccagaaact ttctctgctg cgccctgttc cgcaagaagt
     6601 tgatgctatc gccgtgtggg gacatcgtcc cagcgcggca aaaccggtcg ccatcgccaa
     6661 agcagcggga aaacccgtca tccgtctgga agatggattt gtgcgttcgc tggatcttag
     6721 cgtcaatggt gcgccgccgc tttctctcgt ggtggatgat tgtggcattt actacgatgc
     6781 cagcaagcct tcagcactgg agaaactggt acaggataaa gccggaaata cagctctgat
     6841 aagtaaggcc agagaggcga tgcacaccat cgtgaccggg gatttgtcga aatataacct
     6901 ggcgcctgcg tttgtggctg atgagtcaga atgttcagac atcgttctgg ttgtcgatca
     6961 gacatttaat gatatgtcag tgacgtatgg taatgctggt cagaatgagt tttctgccat
     7021 gctggaggcc gcgatggcgg aaaatcctca agcgacaatt tgggtgaagg tgcacccaga
     7081 tgtactgaaa ggaaagaaaa caggttattt cgccgatctg cgcgccacgc aacgagtacg
     7141 tttgattacg gagaatatca gtccgcagtc gctgttgcga catatttcca gagtttacgt
     7201 cgtgacctcc caatacggct ttgaggcctt actggcagga aaaccagtga catgcttcgg
     7261 tcaaccctgg tatgcaggct ggggcttaac ggacgatcgt catccgcagt cggctttgtt
     7321 atctgcccga cgcggttctg tcacgctgga agaacttttt gccgcagcat acctgcgtta
     7381 ctgtcgctat atcgacccac taacgggggc agcaagtgac ctgtttaccg tactgcaatg
     7441 gctgcaatta ctgcgcaacc atcagcaaca gcgtaatggc tatttatggg cgccaggctt
     7501 aacgctgtgg aagtcagcaa tcctgaaacc tttcttgcgt accgcgacaa acaggctgag
     7561 ttattcacgt cgctgtactg ctgccaccgc ctgcgtggta tggggtgtaa aaggagaaca
     7621 gcaatggcag gtcgaagcgc agcgaaaatc actgccatta tggcgaatgg aagatggttt
     7681 tctgcgttca tccggacttg gctcggacct gctgccatcg ctatcgctgg tgctggataa
     7741 acgcggaatt tactatgacg ccacgcgccc cagcgacctg gaagtgctgc ttaatcacag
     7801 ccagttaacg ctggcgcagc agatgcgagc tgagaagttg cggcaacgac tggttgaaag
     7861 caaactgagt aagtacaacc tgggagccga tttctctctg cctgccgaag ccaaaggtaa
     7921 aaaagttatc ctggtgccgg gtcaggtaga ggacgattcc tctattaaaa caggcactat
     7981 ctcaattaag agcaaccttg agttattacg cacagtacgc gagcgtaatc cgcacgccta
     8041 cattatttat aaaccgcacc cggatgtact ggtggggaat cgcaagggcg atattccggc
     8101 agaactgatt gctgaacttg ctgattatca ggcactggac gcagatatta ttcaatgcat
     8161 tcagcacgca gatgaagtgc ataccatgac gtcgctgtcg gggtttgaag cgttattaca
     8221 tggtaagcac gtacattgtt acggcctgcc cttctatgct ggttggggtt taaccttcga
     8281 tgaacatcac tgcgggcgtc gtgagcgaga attaacgtta gcggatttgc tatatcaggc
     8341 gctgattgtt tatccaactt atatccaccc aacacggcta caacctatta ctgttgaaga
     8401 ggcagcggaa tatttgatcc agacgtcgcg caagccgatg tttattaccc gaaaaaaagc
     8461 gggatgggta atacgctatt accgcaaatt aattatgttc tgcaaggtta gatttggcta
     8521 aacaatttca tagttgacta ttacgcaaat tatttatgca aggtaatgca ctaaccgttt
     8581 tattatccgg taaaaaatat ctgctattgc agggaccgat gggacccttt ttcagtgatg
     8641 tagccgagtg gctggagtca ttaggccgta acgctgtgaa tgttgtattc aatggagggg
     8701 attgttttta ctgtcgccac cgacattatc aggcttacta ccagacaccg aaagagtttc
     8761 ccggttggtt acgggatctc caccggcaat atgactttga taccatcctc tgctttggtg
     8821 actgccgccc attacacaag gaagcaaaat attgggcaaa gtcgaaaggt atccgctttc
     8881 tggcatttga ggaagggtat ttacgcccgc agtttattac cgttgaagaa ggcggagtta
     8941 acgcatattc atcgctaccg cgcgatccgg atttttatcg taagttacca gatatgcctg
     9001 cgccgcacgt tgagaactta aaaccttcaa cgatgaaacg tataggtcat gcgatgtggt
     9061 attacctgat gggctggcat taccgccatg agttccctcg ctaccgccac cataaatcgt
     9121 tttccccctg gtatgaagca cgttgctggg ttcgttcata ctggcgcaag caactttaca
     9181 aggtaacaca gcgtaaggta ttgccgaggt taatgaacga gttggaccaa ctttattatc
     9241 ttgccgtttt gcaggtatat aacgatagcc agattcgtaa ccacagtaat tataacgatg
     9301 tgcgtgacta tattaatgaa gtcatgtact cattttcacg taaagcgccg aaagaaagtt
     9361 atttggtgat caaacatcat ccgatggatc gtggtcacag actctatcga ccattaatta
     9421 agcggttgag taaggaatat ggcttaggtg ggagagtcat ttatgtgcac gatctcccga
     9481 tgccgggact attacgccat gcaaaagcgg tggtgacaat taacagtacg gtgggaatct
     9541 ctgcactgat tcataacaaa ccacttaaag tgatgggcaa tgccctctac gacatcaagg
     9601 gcttgacata tcaagggcat ttacaccagt tctggcaggc tgattttaaa ccagatatga
     9661 aactgtttaa gaagtttcgt ggatatatat tgatgaagac acagcttaat gcagtttatt
     9721 atgggatttt taattttaga aaaataacga attaataaaa catctataat acaaacaacg
     9781 gttgttgtat attatttatt gaattcaatc atttttatac ttaatgtttt tgtaaataat
     9841 aagttgactg tgttgtacta tatta
//
LOCUS       DAZGTJ010000140         9777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000140 DAZGTJ010000000
VERSION     DAZGTJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.140"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(25..627)
                     /gene="fimB"
                     /locus_tag="ACHK8E_004651"
     CDS             complement(25..627)
                     /gene="fimB"
                     /locus_tag="ACHK8E_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP0346819.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2079..2795
                     /gene="nanC"
                     /locus_tag="ACHK8E_004652"
     CDS             2079..2795
                     /gene="nanC"
                     /locus_tag="ACHK8E_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP0346820.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2815..3921
                     /gene="nanM"
                     /locus_tag="ACHK8E_004653"
     CDS             2815..3921
                     /gene="nanM"
                     /locus_tag="ACHK8E_004653"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0346821.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3986..4966
                     /gene="nanS"
                     /locus_tag="ACHK8E_004654"
     CDS             3986..4966
                     /gene="nanS"
                     /locus_tag="ACHK8E_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0346822.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(4974..5624)
                     /locus_tag="ACHK8E_004655"
     CDS             complement(4974..5624)
                     /locus_tag="ACHK8E_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0346823.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            5761..5904
                     /locus_tag="ACHK8E_004656"
                     /pseudo
     CDS             5761..5904
                     /locus_tag="ACHK8E_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(6612..6695)
                     /locus_tag="ACHK8E_004657"
                     /pseudo
     CDS             complement(6612..6695)
                     /locus_tag="ACHK8E_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(6878..7333)
                     /locus_tag="ACHK8E_004658"
     CDS             complement(6878..7333)
                     /locus_tag="ACHK8E_004658"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFP0346824.1"
                     /translation="MPDMRLFKSKNTKLELRIRKPLFARGFRYRLHNSGLPGRPDMYF
                     PKYNAVIFIHGCFWHGHDNGCPISHTPKNNESYWQNKINKNQERDVKNQYVLASKNIR
                     VLIIWECAIKLKSIDFVDFIDYIDEWLRGGANSTQMSAKEPMPTFEFPK"
     gene            7559..8698
                     /locus_tag="ACHK8E_004659"
     CDS             7559..8698
                     /locus_tag="ACHK8E_004659"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP0346825.1"
                     /translation="MSTMKPAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYK
                     HSRKKLGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGK
                     NKGKDGENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYI
                     LHERLINSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYE
                     EDSSMECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKT
                     VGNGVPYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            8634..9707
                     /locus_tag="ACHK8E_004660"
     CDS             8634..9707
                     /locus_tag="ACHK8E_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0346826.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYSSVEELYSL
                     CDRRKPKGINDLFLDSFMEDCVI"
BASE COUNT         3009 a         1998 c         2050 g         2720 t
ORIGIN      
        1 ccatccctgg tatctcaact atctctataa aacagcgtga cgctgtctcc ctctggctct
       61 atcccagatg ccgtaaaaac gccctgcatt gctggcggta taccagacag tatgacgaat
      121 attgcgatgc ccaagataat cctggataag tcgcgtatct attcccatat tcgccaaagc
      181 aaaaccacac gaatggcgta acatgtgcgg atgaatctcc agtgacaacc cggcattacc
      241 accggaagtc gagataatat ggtaaaactg ttgccgagaa agtggattcc ccttacgtga
      301 taagaatacc cactcgctct cagcatgcgg ataagaagta cggatactca accagttttt
      361 taaagcctga atttctttgt tcaatagcgg gtgcgttgtt gaaaagcctt tttttaatcg
      421 atggatatat atacactttg ccttaagatc aatatccgaa atcctcaatc ggcaaatttc
      481 actcgcccgg aaaccatgaa taaaacaaag caaagtcaga caataattac gtgctgcatg
      541 aggcccggta tttgctgctt taaggagtga ttcgatttca ctatgggtca ggaagttcct
      601 ttttttgtta tcagccttat tcttcatcgt tttcccttat aattacagac gcgcactagc
      661 tgtgctgggt tagcaataat ccaacatttt tatcctgatt atgtttataa aagcgaacgt
      721 ttgcttaata actaacgtaa gtggaccagt tcttctgagc gaacttaaat ggagtagcga
      781 tagttactta taataacttt gccatacaaa cgccatgctc ttgcatgcta tgtaccttta
      841 tatatttatc aatctgatac gaatcgaata ataaccctca tcattaatgc aactaatctt
      901 atctatatat catgtgatat gttctgtaac aagtaaaatc aacataaaaa attcagcaaa
      961 atttaatatt cgttcaacta gccgtctcac atatgacatt attagtcaac tgcatttgcc
     1021 acaaaatggc tatttcatgg aacgcagtaa tttcttagtg gctattatca tgctaattct
     1081 tagatcccta gcgattattc ctgcctggtt cgtcagctta ggccatgaga ttgttacatt
     1141 cccttttatc ctggagatat gggggaacgc aggcgacgct ttttcatcta catccgttgg
     1201 taactatggc tttggtcact acgacttaaa ccttcctcaa tcacagctat tcgccgggcg
     1261 atacggcaaa caaactaagt gaaacatcat cctgaaaaca ccaacatcaa caagcctctc
     1321 cagatcgaca tcaggagtga ccagttacaa gccacaaacc aagaaacata tttcatgatg
     1381 agaattatgc tcagcctgat ggcgggtaaa cacttatttt caccaagctc actgagttag
     1441 cttgcaaagc tttctttgtt tagattctaa ataaattttt tataaaacag acagttaaaa
     1501 taaaatcttt aatttttgaa gaccaccgca agtgttcgtc tggcttcaca tggcatctgc
     1561 ctcttagaaa aagatcgaca tattttgtga cacgaattgc aaatctcgtt ttgttgtatg
     1621 gattgcgtga tttttgatct ggtataacag gtataaaggt gcaccgcgat agtcaatgag
     1681 acagggcatc tcgcaatcta tggcaaacat cacttcagtt ctttctcatc gggtgatgaa
     1741 aacgcacttc agtctgaaag gaatatgaaa atgagatcaa cagacactct attttatgac
     1801 tctgggtaaa atggattgag taagtgatat agcttacgaa cattcaaatc aattaaacat
     1861 cagaagagat tttatactca ggtatttaat ctggttctct gtttatttaa ataatgtgga
     1921 aagagaattt tcacaggaga ccttatacaa aaaatataaa ataagctacc cgttgccaaa
     1981 gacactataa gcctggcaaa aaatattaca caacataaat gctaattgtt tatgcgggct
     2041 ttgtattgct ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct aaaatacttt
     2101 ctggcgtatt attactgtgc ttttcgtcac cattaatttc tcaggctgcg acactggacg
     2161 tacgtggtgg atatcgtagc ggaagccatg cctatgagac tcgactcaaa gtcagtgagg
     2221 gatggcaaaa tggatggtgg gcaagcatgg aaagtaatac ctggaatacc attcatgata
     2281 ataaaaagga aaatgccgca ctcaatgatg ttcaggttga agttaattac gcgattaaac
     2341 ttgatgatca atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac
     2401 gctacggacc ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc
     2461 gctatcgtta cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg
     2521 ataacgttca tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg
     2581 catggcaaac gacgctatac agcaaacaga atgattatcg ctatgcaaac cacaagaaat
     2641 gggcgacgga aaatgccttt gttctacaat accatatgac gcccgatatt acgccataca
     2701 tagaatatga ctaccttgac cgtcagggtg tttacaacgg cagagataat ttatcggaaa
     2761 acagttatcg cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat
     2821 aaaacaataa cggcgcttac tatcataatg gcttcatttg ccgcaaacgc gtctgtatta
     2881 ccggaaactc ctgtgccatt taaaagtggt accggagcaa ttgataacga cactgtctac
     2941 attggtttag gtagcgcagg tacggcatgg tacaagctgg atacacaggc caaagataaa
     3001 aaatggacag cgttagctgc attccctggc ggaccgagag atcaagcaac ctctgcattt
     3061 attgatggca atctgtatgt gtttggcggc attggcaaaa acagcgaggg cttgactcaa
     3121 gtatttaatg acgtacacaa atacaacccc aaaaccaata gctgggttaa attgatgtcg
     3181 cacgcgccga tgggcatggc gggccatgtg acttttgtac acaacggcaa ggcttatgtt
     3241 actggcggtg ttaaccagaa tatcttcaat ggctattttg aagatctcaa cgaagctgga
     3301 aaagattcaa ccgctataga taaaatcaac gcccactatt ttgacaaaaa agcagaagat
     3361 tatttcttca ataagtttct gttgtctttt gatccctcaa cacagcaatg gagttacgct
     3421 ggcgaatctc cctggtacgg aacggctggt gcggcggttg tgaataaagg tgataaaacc
     3481 tggcttatta atggcgaagc caaaccagga ttgcgaacgg atgccgtatt tgaacttgac
     3541 ttcaccggtg ataatttaaa atggaataag cttgctcccg tcgcatcacc agatggcgtc
     3601 gctggcggtt ttgcggggat aagcaatgat tctcttatat ttgccggagg ggccggattc
     3661 aaaggttcgc gggaaaatta ccaaaacggt aagaactatg cgcatgaagg cctgaaaaaa
     3721 tcatatagca ctgatattca tctttggcat aacgggaaat gggataaatc tggtgaatta
     3781 tcgcaaggtc gggtctacgg agtatcattg ccctggaata atagtttatt aattattggc
     3841 ggtgaaactg caggcggcaa agcggtgacg gattcagttt taatttctgt gaaggataat
     3901 aaagtcacag tacaaaacta acgcttcagg gcctcggtaa aggggccctg ctatcaattt
     3961 gtcatttgta ttacgcgaaa attatatgaa cgcaataata tcgcccgatt attactatgt
     4021 tcttaccgtt gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga
     4081 cagggaagat gcgcctcatc ccagaattaa acaattagcg agatttgcgc atacgcatcc
     4141 cggaggcccg tcatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt
     4201 tcaggatatg cagggttatc accatcctct ggcaacaaat catcaaacac agtacggcac
     4261 cgttggccag gcactgcata ttgcacggaa attactgccc tttattcctg ataacgcagg
     4321 ggttctcatc gttccgtgtt gccgtggcgg atcggctttt accgcgggca gcgaagggac
     4381 atattcagaa cggcacggag ccagccatga tgcttgtcgt tggggaacgg atactccgct
     4441 gtaccaggat ttagtcagca gaacgcgagc cgcactggca aaaaatccgc agaacaaatt
     4501 cctcggcgta tgctggatgc aaggcgaatt tgacttaatg acctgtgact actcgtcaca
     4561 ccctcaacac tttaatcata tggttgaagc ctttcgtagg gatctaaaac aataccattc
     4621 tcagcttaat aatattactg acgcaccgtg gttttgcggc gataccacct ggtactggaa
     4681 agaaaatttc cctcatgcgt atgaagcgat ttatggcaat tatcaaaata atgttttagc
     4741 caatattatt ttcgtcgact tccagcaaca aggtgaaaga ggactgacga acgcgcctga
     4801 tgaagatccg gacgatttaa gcacgggata ttacggttca gcgtaccggt caccggagaa
     4861 ctggacgacg gcactgcgaa gcagtcattt cagcgcggca gcccgtcggg ggattatttc
     4921 tgacaggttt gtagaagcaa ttttgcggtt ttggcgcgaa aggtgagcgt tcattaattt
     4981 gatttatttg catccatcaa cgcttttaac tcctccggtg aaaacggtgg tttgcgtcgg
     5041 tgcagcatcg catggcaatt agggcacaca ggcagcaaat cattaactgg atccagctgg
     5101 taatcctctt taatggctga aagcggcacc aggtgatgca tatggataaa gccttcagcg
     5161 atatcaccat agatcttcac cagatcaacg ccgcatacgt tgcactggca tccatgatgt
     5221 tcgaccgctt tatctcgggc ctttttatcg cgttcatagc ggtttactgt cacctttatc
     5281 gccgcacctt caaggtatgt ctcctgcgac ggcaactcat ccgggaacag aacgtcggct
     5341 atgggagccg cagcggcttt atcctgatga atatgaaatc cctgctctat gagtagtttg
     5401 acgcatttag attttattcc gccggtgaaa tccgcagggg taaattgcgt ccccgtcatc
     5461 aatgtcgctg cgatgccagc aatcgccttc gaggggtaac gtcggccatg atattcaatc
     5521 tcatacaacc gcgcaggctg gaactgatga gtttctcccg catcccataa atggatagct
     5581 tcgatgatat accgccgcgg aagatgatca gggagtttat tcatggagaa tgtccttata
     5641 cgttctcaga acaggcagca aaaaaacaaa tgagatcaag gtaatatatc ttcacttgca
     5701 ggcttgaaca gcagaaaatt tgcgagcatg atcgcaatta tttcactgcc agggatatca
     5761 atggcttcca gtaaatcgct acagcaggca attgccaata taaaaatctg gcacaagggt
     5821 gaacagcgcg cgccgcataa gccattgtta ttgctatacg tattagcggg atacctgaat
     5881 ggacatccgc gccttttcga ttattgtagt ggaacagtct acgaggatgc agttcgaagt
     5941 gagtaagtaa aggttgctgg aaacgatcaa tgccgccata caatgatttt tgaggatcaa
     6001 caatgagtaa taaaaactac gaaagtcacc gcaaagcgat tgttagtaaa ggtataccac
     6061 ccgctctatt aaataggctc accaattcgg atgttcaggt gatcaatacc ttcttaacac
     6121 gagtgagcaa actggaacta tctcaacaag agaaagactg gatcataaaa atcatctcta
     6181 tggtttagac aatttcagcc ttgtagcctt tcaagggttt tgtgtgggta caaacattat
     6241 cgaatcggga tatagccgca gctgtagctg tatccacatc agtaagttga tgtaggttga
     6301 cttaggttga gcgaaaaata gcgaaaagcc ttgtgtggcg cggatttcag tcataaaaaa
     6361 acgtccgttg acgtctattg atgttccgat ggtgcgaagg tcgaaggccg gactcgcctt
     6421 tgcgtttaat tcgttgaaaa ataatggttt gtgagatttt attttgagga tttgccccca
     6481 aaatgggaca taaaattgaa tactgggtgg aagatcacaa agttgcgtaa ttattttcca
     6541 ggtccgaaac gcattcaaga gcaaggcctc tcccggcact gcgtcagtcg ggatgctaaa
     6601 tctcccacat gtagccctag taaatcgcgc cagaatgtca ggctagtttg caaattactg
     6661 acggcgagcg ttaagtggtt cagagattga agcataggat gcctattgat ggttaataat
     6721 ttaaccaaac actaatagct aataaaaccc cacatggaaa gagatttttt gcgggtcttg
     6781 ttttgtaata aatttcttgg caattacttt atttcatata tatattagca aataacgatg
     6841 ttagatatta ccactcaagg gaacttgtca taataattca tttcggaaac tcaaaagtcg
     6901 gcattggttc ctttgcgctc atctgtgttg aatttgcccc acctctaagc cattcatcga
     6961 tgtaatcaat aaaatcgaca aaatcaatac ttttaagttt tattgcacac tcccatataa
     7021 tcagaacgcg gatgttttta gatgcaagaa catattgatt tttgacatct ctttcttgat
     7081 ttttattgat tttattttgc cagtacgatt cattattttt aggagtgtga gatatagggc
     7141 agccgttatc atgcccatgc caaaaacagc catggataaa aatgactgca ttgtatttag
     7201 ggaaatacat atctggtcta ccaggcaaac cactattatg caatcgataa cgaaaccctc
     7261 tggcaaacaa cggcttcctg attcttaact caagtttggt gttttttgat ttaaataacc
     7321 tcatatcagg catataagtt ccttcggttt ctaaatctat aatagcagct tatactatta
     7381 atcaatatat taacacctaa ctttatagca atgaatacta tctaatctga agtagtgcca
     7441 ggtcttattt gctaatatat taaaaagtaa attgataatt aacaagttat catgtaaaat
     7501 agtaggatcg tacagcgaga tatttagtaa aaatttaagc taacttgata ggtataaaat
     7561 gtccacaatg aaacccgcta tattttcatt cttttcagga tcaggttttc ttgacctagg
     7621 cttcgaaaat gccgggtatg aggtggtttt cgtcaatgaa ttccatcctc ccttcatgga
     7681 cgcgtataaa cactcaagaa aaaaacttgg caaacagccg ccacgctttg ggtatgccca
     7741 agaaagcatt gaaactattg agcattcagt gattaagcat agcatgaaaa cactgaaagg
     7801 tgaaaaccgc ctagtgggct tcattggcgg accaccatgc cctgactttt ctgttgctgg
     7861 caaaaacaaa ggcaaagatg gtgaaaatgg caaattatca caaagttaca ttgacctgat
     7921 aattgaaatg atgcctgatt tcttcctttt tgaaaacgtt aaaggcttat accggactct
     7981 taaacataga gaatttttcg aacaaattaa gaatgaacta gcttcagtag gatatatcct
     8041 gcatgaaaga ttgattaatt caattgaata cggtgcgcct caagatcgag aaaggattat
     8101 tttagttggt tttcttgata agtctttagc caaaaacttc gaatggagtg gccaaatata
     8161 ccccaataag gaagctttcc agttcccttg gccaacaaca aacacatacg aagaagactc
     8221 aagtatggaa tgcccgcaag gtattccaga agaacttact atagaatact ggttcgagaa
     8281 aaacgatgtt accaatcatc caaacgctaa acatgtattt acggcccgag ccggtttagc
     8341 aagattccag tcagtagaag aaggcgacga tagtaaaaaa tcatataaaa ggcttcatcg
     8401 ctggagatac tctccaacag cagcatatgg taataatgaa gttcaccttc acccatacaa
     8461 acctcgtcgt attacggtcg ctgaagcatt ggcaatccag tctttgccaa aaaattttga
     8521 gctaccaggg gatatgactt tgtcgaatat gtttaaaaca gtcggaaacg gagttcccta
     8581 tatcgccgcg ctcggtattg cccaatcaat tttgcaacat ttaggataca gtaatgagga
     8641 taacagcatc gaatctggtt caggcgattt cgagcttacc caaaaatacg ctgtttaact
     8701 atataaacga taggaatagc gggaagattg agattgtacg tatccaacat cccgaaggtc
     8761 ctatcgaaat aaagagattt gatcctaaga aaggtcaaac tcaagcaaca gctaaaacag
     8821 agagtatttc gactcaaatg ctttggcgac ttgcaaatgc actcgaagaa aacagacctg
     8881 tcaacgttga aagggttttc ggtgccagtt acaacacccg ctcggtattg gagagcttgc
     8941 tcgcacatac tccagagttc tattggtgta ggccagccag actggaaatc atgaatgcac
     9001 aaaagtctat aaaaccagga cataagcatc tgatttttct gccagatatg ccacatacta
     9061 atggtttgtt aattgagcac cagactaata ttgttgtctc tgaaatgagc ttcgatgttg
     9121 ttcaccaaag cgttgatatt gagacaatta aaccgactaa aggcatgact attgaagaaa
     9181 aaagacgtca tgctcagata caaattgcct tagtaaaaat aggttattgt ttaggtttag
     9241 atacttgggt agcagccaat gatcgtagct tacaatataa tggtaaagct atagcgcaaa
     9301 tggacggtgt aataagttca ttatctgatg agcaagtact ccaatcgtat aatgatgcga
     9361 ttaaagatgc tcgtcttata gactgcattt ggtttaggga aggccgcatg atgccagcag
     9421 taatggagat tgaacacagt actggtataa aaagtggtct gactcgcatg aaacaattct
     9481 atgatcatgc accgagatta caagatatcc gttggactat cgttgctcct gatgaatatc
     9541 gagcaaaggt gatagaattt gcaaacatgc cccaattcat tgaattaaaa gctaaattct
     9601 tcccttactc ttcagttgaa gaattatact ctctatgcga ccgaagaaaa cctaaaggaa
     9661 taaatgattt atttctagat tcttttatgg aagattgtgt tatatagcta agaacatcaa
     9721 attaacatga acctactgat atatactatc agtaggttta aaatgatttc aaatgtg
//
LOCUS       DAZGTJ010000141         9434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000141 DAZGTJ010000000
VERSION     DAZGTJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.141"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            113..475
                     /locus_tag="ACHK8E_004661"
     CDS             113..475
                     /locus_tag="ACHK8E_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346827.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(578..1036)
                     /gene="ybbJ"
                     /locus_tag="ACHK8E_004662"
     CDS             complement(578..1036)
                     /gene="ybbJ"
                     /locus_tag="ACHK8E_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP0346828.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1033..1950)
                     /gene="qmcA"
                     /locus_tag="ACHK8E_004663"
     CDS             complement(1033..1950)
                     /gene="qmcA"
                     /locus_tag="ACHK8E_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0346829.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2096..2773
                     /gene="fetA"
                     /locus_tag="ACHK8E_004664"
     CDS             2096..2773
                     /gene="fetA"
                     /locus_tag="ACHK8E_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP0346830.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2760..3539
                     /gene="fetB"
                     /locus_tag="ACHK8E_004665"
     CDS             2760..3539
                     /gene="fetB"
                     /locus_tag="ACHK8E_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP0346831.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3602..4456)
                     /gene="cnoX"
                     /locus_tag="ACHK8E_004666"
     CDS             complement(3602..4456)
                     /gene="cnoX"
                     /locus_tag="ACHK8E_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP0346832.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4517..5326)
                     /gene="ybbO"
                     /locus_tag="ACHK8E_004667"
     CDS             complement(4517..5326)
                     /gene="ybbO"
                     /locus_tag="ACHK8E_004667"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0346833.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5316..5939)
                     /gene="tesA"
                     /locus_tag="ACHK8E_004668"
     CDS             complement(5316..5939)
                     /gene="tesA"
                     /locus_tag="ACHK8E_004668"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP0346834.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5910..6596
                     /gene="ybbA"
                     /locus_tag="ACHK8E_004669"
     CDS             5910..6596
                     /gene="ybbA"
                     /locus_tag="ACHK8E_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP0346835.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6593..9007
                     /gene="ybbP"
                     /locus_tag="ACHK8E_004670"
     CDS             6593..9007
                     /gene="ybbP"
                     /locus_tag="ACHK8E_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP0346836.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2190 a         2371 c         2558 g         2315 t
ORIGIN      
        1 aaataaattc tgtttctgga ctaaacgtga agcggttcct gatgtgtgct aaaatgcgtt
       61 tctttgataa atctcaattt ttaagaggtt tacgccaggc aggaaaattt aaatgaatga
      121 atttgagaag atttttaatg aaatgaatct ggaccgggca ttgttaccga tcctttttcg
      181 ttcgaaccgg tcgacagtgt ggaagtattt aagcggcgat agtacggctc ctgcctctgc
      241 catgtcgctg atcatgctgt tgcagttgat ccagaaacga aaccccgatc tgttagccga
      301 atggttaacg ctttctgatt tcaccattcc gccagaagtt tacctggacc aacctgatta
      361 ctggaaaggg tgggtgtata cccaacacaa ggtcaataaa aacgtcctgg aatatctaaa
      421 aaaacactat ccggatgaag accaaaaaag tatgggtaaa ggccgggaag agtaattttg
      481 cctctactcg gctatatgcc ggatgcggcg caagcgcctt atctggccta cgattcgggc
      541 atattttgta ggcatgataa gacgcgccag cgtcgcatca ggcgatgacc gcacggataa
      601 tcagcgttat cccttctatc gcaatcacct caacatgcgt acctgcactg agatcctcgc
      661 tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accacgcccg ttgaccagcg
      721 gagattccag aacaaaacgt cggccaatca actgctgccc gcgctggttt aaatgactgt
      781 cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc caggcggcga
      841 gcagcgtcag gacggcaaac attacccctt gccactccca acccagcggc agcaaccaga
      901 ccaccagacc ggtaatcacc gctgccacgc cgctccacaa cagataacca ttgccgccca
      961 acatctcggc tgccagcagc aaaccgccga gacttagcca gaaaatatgt ggatgaacga
     1021 ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca gctcagcaat
     1081 cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacca ctttgctgtt
     1141 actggacgaa ccgatctgct gtaacgcttc ggtgtatttc tgtgcgacga agtagttcac
     1201 cgcctgaata tcaccggagg caatggcttc agacaccatt ttagtggcgc gggcttctgc
     1261 ttcagcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt cgccttccgc
     1321 tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct gacggatccc
     1381 ttccgcttca aggatgtaag cgcgtttggt acgttccgct ttcatctgcg cgttcattga
     1441 agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga ctttaatccc
     1501 ccacgggttg gtggcctcat cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg
     1561 agagagcatt tcgtcaagtt ccattgaacc cagcacggta cggatgttag tcatggtcag
     1621 gttgatgatc gccagctcca gattgctgac ttcataagcc gcgcgcggcg cgtcaatcac
     1681 ctgaataaag cacacggcgt cgatggtaac gttggcgtta tctttcgaga taacttcctg
     1741 ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc gatccataaa
     1801 cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa aacgttctac
     1861 tgtccactga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac
     1921 aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta ccgtccataa
     1981 tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga gagttccccc
     2041 ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa cagtgatgca
     2101 ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg atgcgaagat
     2161 tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta ccggtccttc
     2221 tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc caaccagcgg
     2281 aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct accgccagca
     2341 agtctcttat tgcgcccaga cgccgacgct gtttggcgac acggtatacg ataatctgat
     2401 ctttccctgg cagatccgta accagcagcc tgacccagcc atttttctcg attttctcga
     2461 acgcttcgcc ttaccggaca ccattttgac gaagaatatc gccgagctat ctggtggtga
     2521 aaaacaacgc atctcattga ttcgtaacct gcaatttatg ccgaaggttt tattgctgga
     2581 tgaaataacc agtgcgctgg acgaaagtaa taaacataac gttaatgaga tgatccaccg
     2641 ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag acgaaattaa
     2701 tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg aagcacgcta
     2761 tgaactcgca taatattact aacgaatcat tagcactggc attgatgctg gtggtggtgg
     2821 caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattctc tggagcgtcg
     2881 ggcgcgcgat aatccagctg attattgtcg gctatgtgct gaagtatatt ttcagcgtgg
     2941 atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg
     3001 cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt gccattacgg
     3061 tcggggcggg aattaccctg gcggtgctga ttctctcagg gtcgattgaa tttatcccga
     3121 tgcaggtgat ccctatcgcc gggatgatag ccggcaacgc catggtagcg gtggggttgt
     3181 gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag gagaaactga
     3241 gtcttggcgc gacgccgaaa caggcttcag cgatattgat tcgcgacagt attcgcgcgg
     3301 ctttaattcc gacggtcgat tcagcaaaaa cggtaggctt agtgagttta ccaggaatga
     3361 tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat cagattatgg
     3421 tgacctttat gctgctctca accgccagct tgtcgaccat tattgcctgc tatttaacct
     3481 atcgtaagtt ttacaattcg cgccaccaat tggtggtgac gcaattgaag aagaaatgat
     3541 gcgatgccgg acgcgcagca tcgcatccgg cgttgtggtt catgtgccga tcaacggaaa
     3601 ctcaatacaa caacgcgtac agctggcggc gatacttcga cgccagtgca tcacccgtac
     3661 ccagcgcagc gaggatctcc tggaatgttt tacgtgtctg accatctgcg gcggtgagat
     3721 ctttacgcag atgcccaaac agtaactcca gcgcctcttc attgcgccca acctggtgca
     3781 gttgcagtgc cagttgtgtc gccagtgcgg catcttctgg attctccgcc acctgctgtt
     3841 gcaactgttg aatttccggc gtatcagccg cctgcttcag cagttcgatt tgcgccacca
     3901 gcccctgata gcgggtgtcc tgatcctgca acggaatggt tttcagcacg gcttctgcat
     3961 cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc tccccgttct
     4021 gattcgacaa ctgccaggcg tctttcagca atggcaaggc gtcggtgtaa ttgccttcct
     4081 gcatcagctg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc actttatcca
     4141 gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc ggttgcccgt
     4201 tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct
     4261 ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc gcgaggcttt
     4321 ccaggatcgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa tagaacagca
     4381 ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta atgttgacaa
     4441 tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac atgggggctt
     4501 aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgcccc ggcagcaggc
     4561 gcttaagcac cattaccgcc caggtcacca gcgttaccgg atagcgcatc ttcggcttct
     4621 cgctaataaa agcatggcgt actttgtcca ccaccgcttc cggtcccaac gtaaagcggg
     4681 cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg ttgtcggtga
     4741 agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccacag tggcgtagct
     4801 ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg taagcaccac
     4861 gacccggcgt ggagattaat cccatcaccg atgaggtcat cacaatacgc ccttcaccgt
     4921 gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg
     4981 aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac atgccgaatc
     5041 cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc tcgtcggctg
     5101 cgcgatcaac actttctggt gaatccagat cgatcaacac gccggtaaat cccatgctgt
     5161 tcatgcgctc aacatcatcc ggtttccggc aacctgccag cacatgaaaa ccctggcgtt
     5221 ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt aagaccgatt
     5281 tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt tactaaaggc
     5341 tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt gggatgaata
     5401 ccgtcatcct gcatccattg tggtttgagg tagacctctt ccataaaaaa gggcagcagc
     5461 ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc attataacgg
     5521 cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt ggcggctttg
     5581 acatcctgca atatctggcg cagcgtttgc tcggtttgtt gtggctgaaa accacgcaaa
     5641 ccgtcattgc cgcccagttc taccagcacc cagcgcggct gatgctgttt cagcagagcc
     5701 ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt gactaccgac
     5761 gttttactct gccacttatc gttcaacaag gcaggccagg ccgcgctggc agacattcga
     5821 tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc ggcacggaag
     5881 gttaacagga ccaggaacag gaagggcaaa tgccagcgga aaacattgtt gaagttcatc
     5941 atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc ggagttgagc
     6001 tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg ggtaagtcaa
     6061 ctttgctggc gatcctcgcc gggctggatg acggcagcag tggcgaagtg agtctggtgg
     6121 gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg
     6181 gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa aacgtcgagc
     6241 ttccggcgct gctgcgcggt gagagtagcg cggaaagtcg taacggggcg aaagcgttgc
     6301 tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt tccggcggtg
     6361 aacagcaacg agtggcgctg gcacgagcct ttaacggtcg acctgatgtg ctgtttgccg
     6421 acgaacccac cggcaatctt gaccgacaga cgggcgataa aattgccgac ctgctatttt
     6481 ccctcaaccg tgagcatggc accacgttga ttatggtgac ccacgacctg caactggcgg
     6541 cacgctgcga ccgctgctta cggctggtga acgggcaatt gcaggaggaa gcatgattgc
     6601 acgttggttc tggcgcgaat ggcgctcgcc gtcgctatta attgtctggc tggcgctaag
     6661 cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg agaagggctt
     6721 aagccagcaa agccgggagt ttatggcggg cgatcgggcg ttgcgcagtt cacgcgaagt
     6781 gccacaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac
     6841 tttcgccaca atgacctttg caggcgacac accgcagctg gcgaacgtca aagcggtgga
     6901 tgatatctac ccgatgtatg gcgatctgca aactaatccc cctggcctga aaccgcaggc
     6961 gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa caggcgacac
     7021 catcgacgtg ggtgatgcca ccttgcggat tgccggagaa gtgattcagg aaccggattc
     7081 cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg atgtcgataa
     7141 aaccggagcc gtgcagccgg ggagtcgggt cacctggcgc tataaattcg gcggcagcga
     7201 gaaccagctc gacggctatg agaaatggtt gttaccccag cttaaacctg aacaacgctg
     7261 gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atagaacgtt cgcaacagtt
     7321 cctgctgctt tcggcgcttc tgaccttgct gctggcggtg gcggcggttg cggtggcgat
     7381 gaatcattac tgtcgcagtc gctacgactt ggtggcgatc ctcaagacgc tgggggcagg
     7441 gcgagcgcaa ctgcgtaagt taatcgtcgg tcagtggttg atggtgctgg cgctttcagc
     7501 cgttaccggt ggtgcgatag gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc
     7561 cgttttgcct gctgcactac cgccagccag cctctggccg tggttgtggg cgctgggcac
     7621 catgacggtc atctcgctgc tggtggggct acgaccgtac cgcttgttgc tggcaacgca
     7681 gcctttacgc gtattacgta atgatgtggt cgcgaacgtc tggccgctga agttttatct
     7741 gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct
     7801 gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg tgctgggctg
     7861 gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctacctctgc gcctggcggt
     7921 tagccgcctg ttacgtcagc cgtggtcaac gttaagccag ctttcggcat tttcgctctc
     7981 ctttatgctg ctggcgctgt tgctggtatt gcgtggcgat ctgctcgacc gctggcagca
     8041 gcagctacct ccagaaagcc cgaactactt tttaattaac atcgccacag aacaggtagc
     8101 accgctaaaa gcgttcctcg cggaacatca gatagtcccg gaatcgtttt atccggtggt
     8161 gcgggcgcgg ctgacggcga ttaacgataa gccgacagaa ggcaatgaag atgaggcgct
     8221 caatcgcgaa ctcaatctta cctggcaaaa tacgcggccc gatcataatc cgattgtcgc
     8281 cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa gaggggctgg cgaaacgctt
     8341 aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat acccaggagt tccgcgctaa
     8401 agtgaccagc ctgcgcaaag tggactggga aagtctgcgg cctaatttct attttatttt
     8461 ccctgaaggg gcattagacg ggcaaccgca gagctggctt accagtttcc gctgggagaa
     8521 tggcaacggc atgttgacgc aactcaaccg ccagttccct actattagcc tgttagacat
     8581 tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg cgctggaggt
     8641 gatggtggtg ctggtcaccg cctgcggtat gttgctgttg ctggcacagg tgcaggtggg
     8701 aatgcgtcag cgtcatcagg agctggtggt gtggcgcaca ctcggtgcgg ggaaaaaact
     8761 gctgcgtacc acgttgtggt gtgagttcgc catgcttggg tttgtttctg gcctggtagc
     8821 cgcaattggt gcggaaacgg cgctggcagt gttgcaggcg aaagtgtttg attttccgtg
     8881 ggagccagac tggcgattgt ggattgtgct gccgtgcagc ggtgcactgc tgctgtcgct
     8941 tttcggcggc tggctgggtg cgcgactggt taagggtaag gcgctgttca ggcagtttgc
     9001 gggatgatga gagtaaataa ggggcgttct ggttgaatgg aacgccttgt ttaaattaat
     9061 tctacaatat gtttcattga tgatgttatt gaattggtga tttcctatct ttctattgct
     9121 gatattaatc tgaatcggtg gagtttaatg attttaaata tagccctaca aaaataactc
     9181 agactaataa aatcataaat catatgcgtt gaatggatat tatccatata gtgaatttgt
     9241 tgataatgaa ttcatctgtg ctaaaaatgt tagtttaata aaatattgaa agtgacctgt
     9301 aataacagtt gttgttgatt gagaacaaat aagtttatgt gaaaaatata taaatacatt
     9361 agctggtctt gtgtgtcatt ttattttttt gttgctaaca cagggatatg aacaataact
     9421 aaaagggcac ttta
//
LOCUS       DAZGTJ010000142         9371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000142 DAZGTJ010000000
VERSION     DAZGTJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.142"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..468
                     /locus_tag="ACHK8E_004671"
     CDS             <1..468
                     /locus_tag="ACHK8E_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0346837.1"
                     /translation="KGGWNFYQYPLNPVSGFDPLGLKVSFQGDESTQKTLKEAYKAVA
                     ETKFGHKITEELESSEHEYIFRGLRKGINQTCYDDTEYSFYIDIDNDHSSCVYQGKNK
                     ACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVDKYENPFRKELGLPARMKY"
     gene            526..936
                     /locus_tag="ACHK8E_004672"
     CDS             526..936
                     /locus_tag="ACHK8E_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346838.1"
                     /translation="MKVCLYFISVFFSSCCIADVFHYRMPFRIQTYSPVTKSKIENFA
                     IKDGAQKLLQINREKLLMLLNRSSNECYHFDDKNVRIYIIDGKDRYYIDKNGVVSYGV
                     QDNVICSNNKINEDSIEKILLEGNIDFWKKLKIK"
     gene            1305..1826
                     /locus_tag="ACHK8E_004673"
     CDS             1305..1826
                     /locus_tag="ACHK8E_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP0346839.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYKIDAGLSESYTCYLL
                     SKGKIIRPYLKNLNPHQLVADCIETVNKIKDKNKKIIDIDSVNICNDNKNINWRVNST
                     IVAIDDSIKCIDE"
     gene            1989..2194
                     /locus_tag="ACHK8E_004674"
                     /pseudo
     CDS             1989..2194
                     /locus_tag="ACHK8E_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(2283..3419)
                     /gene="yibH"
                     /locus_tag="ACHK8E_004675"
     CDS             complement(2283..3419)
                     /gene="yibH"
                     /locus_tag="ACHK8E_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0346840.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(3422..3784)
                     /gene="yibI"
                     /locus_tag="ACHK8E_004676"
     CDS             complement(3422..3784)
                     /gene="yibI"
                     /locus_tag="ACHK8E_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0346841.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            4321..6234
                     /gene="mtlA"
                     /locus_tag="ACHK8E_004677"
     CDS             4321..6234
                     /gene="mtlA"
                     /locus_tag="ACHK8E_004677"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP0346842.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            6599..7747
                     /gene="mtlD"
                     /locus_tag="ACHK8E_004678"
     CDS             6599..7747
                     /gene="mtlD"
                     /locus_tag="ACHK8E_004678"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0346843.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            7747..8334
                     /gene="mtlR"
                     /locus_tag="ACHK8E_004679"
     CDS             7747..8334
                     /gene="mtlR"
                     /locus_tag="ACHK8E_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP0346844.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(8345..8554)
                     /gene="yibT"
                     /locus_tag="ACHK8E_004680"
     CDS             complement(8345..8554)
                     /gene="yibT"
                     /locus_tag="ACHK8E_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP0346845.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            8838..9200
                     /gene="yibL"
                     /locus_tag="ACHK8E_004681"
     CDS             8838..9200
                     /gene="yibL"
                     /locus_tag="ACHK8E_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP0346846.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         2612 a         2134 c         2226 g         2399 t
ORIGIN      
        1 aaggggggat ggaattttta tcagtatccg ttgaatccgg tatctggttt cgatccatta
       61 gggttaaaag tgtcttttca gggagatgaa agtacacaaa aaactttaaa agaagcatat
      121 aaagctgtag cagaaacaaa gtttggacat aaaataacag aagaattaga gtcatcagag
      181 catgaatata tatttagagg attaagaaaa ggcataaacc aaacatgcta cgatgatact
      241 gaatatagtt tttacattga tattgataac gaccatagct catgtgtata tcaaggaaaa
      301 aacaaagcat gtgccatgaa acccactctg ttgagtgtgg ttcttgcgca tgaaatgggg
      361 catgcaaagg gaatgaaaga cgatggaact gactcgatgg ctaatgttga taaatatgaa
      421 aacccatttc gtaaagagtt gggcttgcca gcaagaatga aatattaaac atttttatat
      481 tgcttttggg tatgttttta atatatcacc agtttaggag atggtatgaa agtatgtctg
      541 tattttattt ccgtattttt ttcatcttgc tgtatagctg atgttttcca ttataggatg
      601 ccttttcgca tccaaacata ttcccctgtc actaaaagta aaattgaaaa ttttgcaata
      661 aaagatggtg cacaaaaatt attacagata aatcgtgaga agttattaat gttactgaac
      721 aggtctagta atgagtgtta ccattttgat gataaaaatg ttaggattta tattattgat
      781 ggtaaagaca gatattatat tgataaaaac ggggtggtgt catatggcgt tcaagataat
      841 gtcatatgct ctaataataa aattaatgaa gattccatag aaaaaatttt attagaaggg
      901 aatattgact tttggaaaaa actgaaaatt aagtgaaatg aacgaaagag aaaattaaca
      961 atgagcggaa aaccagcggt atactaaggc gatgccatgt tgaaagatag tccaatagtc
     1021 taaagtggag aactcaatga agcaaaccgg acgaggcttg cttaacagtc ctggagcaac
     1081 caatagaaaa cctaatggta tacgtttagt accgtcagca aatacagaaa cgtataatcg
     1141 tgatggattt ttaacacata gttgtttaaa cgcttttggt ccatctttag gacctcgatt
     1201 ctgttccgag ggttgtataa ctggttcatc taatgacatg caaaaattaa atgagctaat
     1261 attttcagag ccagatagca cattaacagt gacggactag aaatatggca aggttattaa
     1321 caattgtgct ttttttctct atttctgcga atgcagttac tgtaaaccaa tttcagagag
     1381 agttggattt ggcagacaag acaatattat gggcactaat atcagccagt acccaagagg
     1441 gacgaaaagc gtgttcactt agctattttt catgtaaagc cgcagaggct gaactcggac
     1501 tggcatatat ggctgctaat gataacaagg catttctcac atccttatct agaataatga
     1561 tgtacaaaat agatgctgga ctttccgaat cctatacatg ctatttatta agcaagggaa
     1621 agatcataag accatatctg aagaatctaa atccacacca acttgtcgct gattgcattg
     1681 aaactgtaaa taaaataaaa gataaaaata aaaaaatcat tgatatagat tcagttaata
     1741 tttgtaatga taataaaaat ataaattgga gagttaatag tactattgta gccattgacg
     1801 attctataaa atgcatcgat gaataacaac ttaattatat ttaaaaacat attaataact
     1861 cactctccag gagtttttta acttagtgtt ataatgaata cgataattaa aagtgaagag
     1921 aaaaatagtt gtatacatat attccatgaa cctacattgt gatctataac ttaaatattt
     1981 ttaaatgtga ctgtcatcct tcagataata aagccatcat cgcaatagat ggaaaagcac
     2041 tcctgctctt ttttgattag agccgtggca gaggaacaac tcatatcatc attgcgatct
     2101 cgacaatgta tagtctgatc cttcggcaga tcaagacgga taaaaaatcc aatgagttca
     2161 cactcatccc gaacttctta acataatgga tattaacgaa aaatcatcac agccgatgaa
     2221 ataatatgca aaaataatta gtccttacaa ggagcaaagt tccataatct cccccctccc
     2281 cctcaatgat ccaaataaag ataatgcatc caactggtca ttctcagcag cactttccgc
     2341 atcaccgaaa catggctgaa atggtcggag taaactgcca cttgggcgta gatgccgtcg
     2401 ggtaaggcat cgatatcatc gttagggtcc agttcaatgg ttcccagcac accgtccgtg
     2461 ccaggcacga ccgttaatga ttgcaatacc ccctgcgcct gataagaacc gcctggcacg
     2521 acaggtaaaa tactagtcag tttgccgtga aacacctgcc caggtagcgc gttaaacacc
     2581 acttccgcat catcgccggg tttcagacgt aacagcgagt tttgccgaaa ttgggcgaca
     2641 atttgccgtt tttgctcggg gatgaaaacc atcaccggac gcaacggcaa ggcagctgcg
     2701 tatgtacctg ggcggatcag tacctgagta acgtagccat tgcttggtgc gcgaatgaca
     2761 gtctgctcaa ggttatattt tgcttcagta agttgcgctc ttaagctcac aatctgcgat
     2821 tgctcgccgt taaccatact gtcgagctgg ctctggatct gcgcctgctc cgccaccgag
     2881 cctttcacca gcgcgtcctg cgcgaggaaa ttttgccgcg catcgtcgat gtcacgttcc
     2941 gagaacggat tcaccgccgc ctggctgcct ttcaggtaac gttgataatt tttaaacaga
     3001 cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg cttcagtgag ctgcgcgcgc
     3061 agcgtcttta tattatgcgt cgccgtcatc aggtcagcct gaagtctgtc aactcgcgcc
     3121 tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct tttgaataag ctgattattt
     3181 ttgtcggtga cttcagtaac aattcccgtc acctgtggcg tgatggggat cgctatcact
     3241 gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat caaaccactc
     3301 accagaaaca cgcctcccag cgccgcggtc gccagcgtcc actgattcac cggaatgcgg
     3361 aagattttaa agaccgccca cgccagcgcc acgtaagtta aaacaatcaa tagatccata
     3421 tttagatctc cgggaacgtg gaatagtcag tctttttctc ggcggcgagt tgatgctcca
     3481 gccgggaaat tcggtcagaa agtgcggcga tttccgggtc agtcgctttc tcctgcgacg
     3541 caacatgtga ctgcataccc cagccacgct ccggttgata gagcgtcgcc cagatccaca
     3601 ggaacggcca gataacatgc agagtaaaca ggctcaccca gcccgccgta tgaatagcgt
     3661 cggcatgggg atgattgcgc tttttggcaa tcagatatgg gatgtcatgt atcgcgatga
     3721 ttccataaaa aatcaccaga aagacgaaga tcagcactcc caacgcgaaa tagtttagaa
     3781 acatatctgc ctcggattca cgtttatcag tgttgttttt tggctggcag ccagaaggga
     3841 gtcaggctga tattttgaca ataatccggg ttcgcgattc tcgccataac accaaagaat
     3901 aatttttaga ggtgatgagt tgcttagtta cataacgatt ttatgacgaa ggcataacat
     3961 gctgtagatc acatcaggtg aacgccgtaa gaaaatatct tgtgattcag atcacaaaga
     4021 tccaacaaac caccaaaaca aaaatgtgac attagtcaca tttaaatgcc atttttagcg
     4081 aaaatcgccg tcttgttgct tttttacaca agcattttgt gatgagcatc acgtcaatta
     4141 cctctctacc ccctatattt atgtgaccca tatcacacaa aaggccgtcg actggacagt
     4201 taaccgattc agtgccagat ttcgcagtat ctacaaggtc cggctacctc tgccgccaca
     4261 ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt
     4321 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg
     4381 atgccaaata tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca
     4441 gggtggttac cgaacgagac gctggcgaag ctggtcgggc cgatgatcac ttatctcctg
     4501 ccgctgctga tcggttatac cggtggtaag ctggtaggcg gcgaacgtgg cggcgtagtc
     4561 ggtgccatca ccaccatggg cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct
     4621 atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg ggtagacggt
     4681 aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg
     4741 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg
     4801 ctggctgcgg gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt
     4861 gttgaaccgg cgaaaatcct gttcctcaac aacgccatta accacggtat cttctcgccg
     4921 ctgggcattc agcagtccca tgaattgggt aaatccatct tcttcctgat tgaagctaac
     4981 ccaggtccag gtatgggcgt gctgctggcg tacatgttct ttggtcgtgg cagtgctaaa
     5041 caatctgcgg gcggtgcggc aatcatccac ttcctgggtg gtatccacga aatttacttc
     5101 ccgtatgtgt tgatgaatcc gcgtctgatc ctggccgtta tcctcggcgg tatgactggc
     5161 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc
     5221 cttgctgtac tggcgatgac accaaaaggt gcttatttcg ctaacatcgc aggtgtgtgt
     5281 gcggcgatgg ctgtctcctt cgttgtctct gccattctgc tgaaaaccag caaagtgaaa
     5341 gaagaagatg atattgaagc agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa
     5401 ggtgcgtctc cgctgtctgc gggcgatgtg actaacgacc tgagccacgt acgtaaaatc
     5461 atcgttgcct gtgacgccgg tatgggttcc agtgcgatgg gcgcaggtgt gctgcgtaag
     5521 aaaattcagg atgcaggtct gtcgcagatt tcggtcacta acagcgcgat caacaacctg
     5581 ccgccagatg tggacctggt catcactcac cgtgacctga ccgaacgcgc tatgcgccag
     5641 gttccgcagg cacagcatat ttcgctgacc aacttcctcg acagcggctt gtacaccagc
     5701 ctgaccgaac gtctggttgc tgcccagcgc catactgaaa acgaagtaaa agtgaaagac
     5761 agtctgaaag acagctttga cgattccagt gctaacctgt tcaagctagg cgcggagaac
     5821 atcttcctcg gtcgcaaagc ggccaccaaa gaagaagcga ttcgttttgc tggcgagcag
     5881 ctggtgaaag gcggttacgt tgagccagaa tacgttcagg cgatgctgga tcgtgaaaaa
     5941 ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcaaaa
     6001 gatcgcgtac tgaaaacggg cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt
     6061 gaagaagaag atgacattgc ccgtctggtg attggtattg ctgcccgtaa caacgagcac
     6121 attcaggtta tcaccagcct gaccaatgca ctggatgatg aatccgtcat cgagcgtctg
     6181 gcacacacca ccagcgtgga tgaagtgctg gaactgctgg caggtcgtaa gtaatacaat
     6241 ctcaccctct ccacatggag aaggtggggc ttattgcctg atgcgctacg cttatcaggc
     6301 ctacaaaaca aagcacagtt tgttgaattt gcacgttctt gtaggccgga taaggcgttt
     6361 acgccgcatc cggcgctgcc cctctccaca tggagaagat gggctaattg cctgatgcgc
     6421 tacgcttatc aggcctacaa aaacaaagca caatttgttg aatttgcacg ttcttgtagg
     6481 ccggataagg cgtttacgcc gcatccggcg cggcccctct ccacatggag agggtttggg
     6541 tgagggaaaa gcctcacccc agccctctcg ggtaaaaaca ttgatgaagg ttaatactat
     6601 gaaagcatta cattttggcg caggtaatat cggtcgtggc tttatcggta aactgctggc
     6661 agacgcgggt atccaactga cgtttgccga tgtcaatcag gtggtacttg atgccctgaa
     6721 tgcccgtcat agctatcagg ttcatgtggt cggtgaaacc gagcaggtag ataccgtttc
     6781 cggcgtcaat gccgtcagca gcattggtga tgatgtcgtt gatctgattg ctcaggttga
     6841 tttagtcact accgccgttg gcccggttgt gctggaacgt attgctccgg caatcgccaa
     6901 agggctggtg aaacgtaaag aacaaggtaa tgaatccccg ctgaacatca tcgcttgtga
     6961 aaacatggtc cgcggcacca cacagctgaa aggccatgtg atgaacgccc tgccagaaga
     7021 tgccaaagcg tgggtagaag aacacgttgg ctttgtcgat tccgccgttg accgcatcgt
     7081 gccgccttcg gcttctgcaa ccaacgatcc gctggaagtg acggtagaaa cattcagcga
     7141 atggattgtc gataaaacgc agttcaaagg cgcgctgcca aacatccctg gcatggaatt
     7201 aaccgacaat ctgatggcat ttgtcgaacg taaactcttc accctgaata caggtcatgc
     7261 tataaccgcg tacctcggaa aactggccgg tcatcagacc attcgtgatg cgattctcga
     7321 cgagaaaatc cgcgcggtgg taaaaggtgc gatggaagaa agtggtgcag tattgatcaa
     7381 gcgctacggc tttgacgcag acaaacatgc ggcgtacatc cggaaaatcc tcggtcgttt
     7441 cgagaacccg tacctgaaag atgatgtaga gcgcgtaggc cgtcagccgc tgcgtaaact
     7501 gagtgctggc gaccgtctaa tcaagccact gctcggaacg ctggaatatg gtctgccaca
     7561 taaaaacctg attgaaggta ttgccgctgc aatgcacttc cgcagtgaag atgatccgca
     7621 ggctcaggaa ctggcagcac tgatcgctga caaaggtccg caggcggcgc tggcacagat
     7681 ttccggtctt gatgccaaca gcgaggttgt atccgaggcg gtaaccgctt ataaagcaat
     7741 gcaataatgg tggaccaggc gcaggacacc ctgcgcccga ataacagatt gtcagatatg
     7801 caggcaacaa tggaacaaac ccaggccttt gaaaaccgtg tgcttgagcg tctgaatgct
     7861 ggcaaaaccg tgcgaagctt tctgatcacc gccgtcgagc ttttgaccga ggcggtaaat
     7921 cttctggtgc ttcaggtatt ccgcaaagac gattacgcgg tgaagtatgc tgtagaaccg
     7981 ttactcgacg gcgacggtcc gctgggcgat ctttctgtgc gtttaaaact catttacggg
     8041 ttgggcgtca ttaaccgcca ggaatacgaa gatgcggaac tgctgatggc attgcgtgaa
     8101 gagctaaatc acgacggcaa cgagtacgcg tttaccgacg acgaaatcct tggacccttt
     8161 ggtgaactgc attgcgtggc ggcgttacca ccgccgccac agtttgaacc tgcagactcc
     8221 agtttgtatg caatgcaaat tcagcgctat caacaggctg tgcgatcaac aatggtcctt
     8281 tcactgacag agctgatttc caaaatcagc ttaaaaaaag cctttcaaaa gtaagtactt
     8341 tcgcttactt ctcctctacc tgtttcggac gataaagctg acgataatac tccagacgtt
     8401 gtagatataa cggcacgctt tcatcaggta ttccggacgg aatcgcgtta cggggaggta
     8461 gttttttcag atactcccgg aacgcctggc tcgatgccat gaaatctacg gctttatcga
     8521 taagaagcgg aacgttttca cctaatttgc ccatgatgat atctccgtat taccccgcgc
     8581 cgggaatgcg cggccgccaa ttttagttta gatcccggta taaatgacat taaggaaaac
     8641 gtgctcaatc ctcaataatc acctgcttct gttgactatc ttttttcttt aaatttctta
     8701 attaatcaat aaattgcgta gtttttaagc atgttaatgt ttacgttata gaactattgc
     8761 aatgtcatca gttgtgaaca aagcacctgg tcgcgcatac taggggctat aaatttatct
     8821 ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc
     8881 gtctggacgc catccgccac cagcaggccg atctgtcgct ggttgaagcc gcagacaaat
     8941 atgccgaact ggaaaaagag aaagccactc tggaagcaga aattgctcgc ctgcgcgaag
     9001 ttcacagcca gaaactcagt aaggaagcac aaaagctgat gaagatgcca ttccagcgcg
     9061 cgattaccaa aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg
     9121 tcgttgtgca cccaatgacc gcgctgggcc gcgaaatggg cctgcaagag atgacagggt
     9181 tctcaaagac tgcgttttaa gttcctttct cgtcgctgta atcacagcct ctcaccattt
     9241 actcgctcct gctggcacat gcaggagcct gtttcagaga tcgaatattt ataacgttct
     9301 taacattata tattcaatct cctctattct ttttagcgca catgccaggc taaattctga
     9361 aaatcaggta c
//
LOCUS       DAZGTJ010000143         9357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000143 DAZGTJ010000000
VERSION     DAZGTJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.143"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..747
                     /locus_tag="ACHK8E_004682"
     CDS             <1..747
                     /locus_tag="ACHK8E_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0346847.1"
                     /translation="VTAGIYGAAGHSSVDVKDDDASRAGTVRDDAGSLGGYLNLTHTS
                     SGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETALPFSITDNLMLEPQLQYTW
                     QGLSLDDGQDNAGYVKFGYGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVS
                     ELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENI
                     TLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            868..3384
                     /locus_tag="ACHK8E_004683"
     CDS             868..3384
                     /locus_tag="ACHK8E_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346848.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDATLQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRQEEALLPELPA"
     gene            3460..3921
                     /locus_tag="ACHK8E_004684"
     CDS             3460..3921
                     /locus_tag="ACHK8E_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFP0346849.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            4057..4146
                     /locus_tag="ACHK8E_004685"
     CDS             4057..4146
                     /locus_tag="ACHK8E_004685"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0346850.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            4324..5145
                     /locus_tag="ACHK8E_004686"
     CDS             4324..5145
                     /locus_tag="ACHK8E_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0346851.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            5145..5345
                     /locus_tag="ACHK8E_004687"
     CDS             5145..5345
                     /locus_tag="ACHK8E_004687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346852.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            5479..5952
                     /locus_tag="ACHK8E_004688"
     CDS             5479..5952
                     /locus_tag="ACHK8E_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0346853.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            5967..6443
                     /gene="radC"
                     /locus_tag="ACHK8E_004689"
     CDS             5967..6443
                     /gene="radC"
                     /locus_tag="ACHK8E_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0346854.1"
                     /translation="MQQLSFLPGAMTPGELSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            6512..6733
                     /gene="yeeT"
                     /locus_tag="ACHK8E_004690"
     CDS             6512..6733
                     /gene="yeeT"
                     /locus_tag="ACHK8E_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0346855.1"
                     /translation="MKIVTRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            6809..7177
                     /gene="cbeA"
                     /locus_tag="ACHK8E_004691"
     CDS             6809..7177
                     /gene="cbeA"
                     /locus_tag="ACHK8E_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP0346856.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            7266..7643
                     /gene="cbtA"
                     /locus_tag="ACHK8E_004692"
     CDS             7266..7643
                     /gene="cbtA"
                     /locus_tag="ACHK8E_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFP0346857.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRVTG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            7640..8128
                     /locus_tag="ACHK8E_004693"
     CDS             7640..8128
                     /locus_tag="ACHK8E_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0346858.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAGCLPGLPTF
                     DW"
     gene            8140..8337
                     /locus_tag="ACHK8E_004694"
     CDS             8140..8337
                     /locus_tag="ACHK8E_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0346859.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            8422..9267
                     /locus_tag="ACHK8E_004695"
     CDS             8422..9267
                     /locus_tag="ACHK8E_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0346860.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKNEVFERGVINVFRGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLNSGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     QLSAI"
BASE COUNT         2255 a         2501 c         2468 g         2133 t
ORIGIN      
        1 gtgaccgcag ggatatatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
       61 gcctcccgtg ccggcacggt ccgggatgat gccggcagcc ttggcggata cctgaatctg
      121 acacatacat cctccggcct gtgggctgac attgtggccc tgggaacccg ccacagcatg
      181 aaagcgtcaa cggacaataa cgacttccgc gcccgggggt ggggctggct gggttcactg
      241 gaaaccgctc tgcccttcag tatcactgac aatctgatgc tggagccgca actgcagtac
      301 acctggcagg gactctccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      361 tatggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa cgatatgacc
      421 tttggtgaag gcacctcatc ccgtgacacc ctgcgcgaca gtacaaaaca cggtgtgagt
      481 gaactgcctg tgaactggtg ggtacaacct tctgttatcc gcaccttcag ctcccggggg
      541 gacatgagca tgggtacagc tgcagccggc agtaacatga cgttctcacc gtcccggaat
      601 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
      661 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg gtataacggt
      721 caggccacac tgaatgtgac cttctgacag aaccatcgcc tctctgtggt cccggtcatc
      781 atgaccggga cccggaccgg cgcaacggat cttcaacgcc acattcgctg gcattaacaa
      841 taacatgata ttcatcacgg agtgactatg ttacagatag tcggcgcgct gatcctgctg
      901 atcgcaggat ttgccattct tcgccttttg ttcagagcat taatcagcac ggcttctgcg
      961 ctggcagggc tcatattgct gtgtctgttc ggcccggcct tactggctgg ctatatcacc
     1021 gaacgcataa cccggttgtt ccatattcgc tggctggcag gcgtatttct gacgattgcc
     1081 ggaatgatca tcagcttcat gtggggactt gatggtaaac atatcgcgct ggaggctcac
     1141 acctttgact ctgtgaaatt tattctgacc accgctctcg ccggtggtct gctggctgtt
     1201 cccctgcaga tcaaaaacat tcagcagaac gggatcacac cagaagatat cagcaaggaa
     1261 attaacgggt attactgctg tttttatact gcctttttcc ttatggcgtg ttctgcatgc
     1321 gcaccattga tcgcgttaca gtacgatatt tcaccgtcac tgatgtggtg gggcgggttg
     1381 ttgtactggc tggctgcatt agtgacgctg ctatgggcgg ccagccagat ccaggcgctg
     1441 aaaaaactga cctgtgccat cagccagaca ctggaagaac aaccggtgct caacagtaaa
     1501 tcgtggctga ccagtttgca aaacgattac agccttcctg actcactgac ggagcgcatc
     1561 tggctgacgc tcatttctca acggatttcc cggggagagc tgagggaatt tgaactggca
     1621 gacggaaact ggttactgaa caatgcctgg tatgaaagaa acatggcagg gtttaacgaa
     1681 cagttgaaag agaacctgtc attcacacct gatgaactga aaacgctctt ccggaaccgc
     1741 ctgaatttat caccggaagc gaatgacgat tttctcgatc gttgcctgaa cggcggtgac
     1801 tggtatccct tttcagaagg tcgccgtttt gtatcattcc atcacgtgga tgagcttcgt
     1861 atctgtgcct cctgcgggct gacagaagta catcatgccc cggaaaatca taagccggat
     1921 ccggaatggt actgctcctc tctttgtcgc gaaacagaaa cactgtgtca ggaaatttat
     1981 gaacgccctt acaacagctt tatttccgat gcaacggcga atggtctgat tctcatgaaa
     2041 ctgccggaaa cctggagtac aaatgagaaa atgtttgctt ccggagggca gggacatggg
     2101 tttgccgctg aacggggaaa ccatattgtc gacagagtcc gtctgaaaaa cgcacggatc
     2161 ctcggtgata ataatgccag aaatggagca gacagactgg tcagcggaac agaaatccag
     2221 acgaaatatt gttcaactgc agcccgtagc gtcggtgcgg cattcgacgg acaaaacgga
     2281 cagtatcgtt acatgggaaa taatggcccc atgcaactgg aagtcccccg tgatcagtat
     2341 gccggcgctg ttgaaaccat gaggaataag atccgcgaag gtaaagtacc cggtgtaacc
     2401 gatcccgcag aagcgtcccg gctgattcgt cggggacatc tgacttatac tcaggcccgt
     2461 aatatcaccc ggttcgggac catcgaatcg gtcacttatg atattgccga gggttcggtt
     2521 gtcagtctgg cggccggagg gatcagtttt gccctgacgg catcggtctt ctggctcagc
     2581 accggcgatc gcgatgctac cctgcagaca gctgctgtcc aggcaggaaa aaccttcacc
     2641 cgcacactgg ctgtctacgt cacaacccag caactccacc ggctcagtgt tgttcagggt
     2701 atgctgaagc atattgattt ttcgacggcc agcccgactg tccggcaggc gcttcagaag
     2761 gggaccggtg caggaaatat cagtgccctg aacaaagtga tgaagggtac gctggtgaca
     2821 tctctggtac tggtcgctgt cacaaccggc cctgacatga tcaaaatgtt gcggggacgg
     2881 atctccggtg cgcagttcat caggaatctt gccgtggcat cttccggtgt ggcaggtggt
     2941 gctgtcgggt cagtggcggg cgggatactg ttcagtccac tgggaccatt tggtgcactg
     3001 acagggcgtg tggctggcgg tgttctgggg ggaatgattg cctccgctgt atcaggaaaa
     3061 attgccggag cgctggttga agaagatcgc gtcaaaattc tggcaatgat tcaggagcag
     3121 gtgacatggc ttgccggcag tttcctgctg accggacatg agattgaaaa tctgaacgcg
     3181 aatctggccc gtgttatcga tcagaatgct ctggagatca ttttcgccgc cggtatacaa
     3241 caacgggccg cgaccaatat gttaatcaaa ccactggtgg tcagtatcat caggcaacgc
     3301 cccgtcatgg aatatgatgc atcccatctc ggcaagatgg ttaaccgaca ggaagaagca
     3361 ttactcccgg agttaccggc ataacggcta ttctgcgcct tttacgtttt atcacctgat
     3421 ttgtcagtgt catcgtcatg actcagagag gtaaatacca tgattcacct gttcaaaacc
     3481 tgcatgatta ccgccttcat tctggggtta acgtggtctg ccccactccg ggcacaggat
     3541 caacgttaca tcagtatacg caatacagat acgatatggc tcccgggaaa tatttgtgct
     3601 taccagttcc ggctggataa tggcggaaac gatgaaggat ttggccccct caccatcact
     3661 ctgcaactca aagacaaata tggtcagacg ctggtgacca gaaaaatgga aacggaagcc
     3721 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
     3781 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
     3841 ctgccgttat cggttttcga tccccaggac taccatccac tgctgattac cgtttccgga
     3901 aaaatgttaa ctgatccctg aagtcagttt catgcttcag cctctgccac aacgtatggc
     3961 agaggctatt tatcaggaga aaccagatat gccgggttac acacagagta tgtcctggct
     4021 gaaggatctt tctcatacgg gcaggcggtt gctgtcataa ccgcttaccg caatgtgttt
     4081 atccaggatg acccgggaat gcatttccgt cgggttatcc gcaacgcgga aggtcagcga
     4141 cgctgacgct gccggaactc tgaagctgat gccggaaaac agcttaatgc gtggctcgcc
     4201 agtggagggc ttctcagaca ataaacgtca ttgatttcgt ccatcaggcc gcgtcttctc
     4261 cgggagacgc ggctttttca tttattgcac cagtaaatct taaccaccga taaggagcaa
     4321 agtatgcgat tagtcagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
     4381 ctgacacatg aagagctgat gcaccatgtg ccggggattt tcggggaaga gaaacacaca
     4441 tcccgaagcc agaactatac gtacatcccc accatcaccg tactggaaag tctgcagcgg
     4501 gaaggcttcc agcccttttt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga
     4561 tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct
     4621 gaaattattc tgctcaactc ccatgacggt acctccagct accagatgct gccgggttac
     4681 ttccggttcg tctgccagaa cgggtgcgtc tgtggtcagt ctctggggga agtgcgtgtt
     4741 ccacaccggg gaaatgtggt gcagaaagtc attgaagggg cttacgaggt ggtgggcgtt
     4801 tttgaccgga ttgaagagaa acgtgatgcc atgcagtcgc tggtcctgcc gccaccggca
     4861 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc
     4921 accaccgccg acattctgac gccacgacgt cgggaggatt acggtaaaga cctgtggagc
     4981 gcatatcaga ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa
     5041 ggaaaacgta tccacacccg tgccattcac agcatcgaca ccgatattaa gctcaaccgc
     5101 gcattgtggg tgatggcaga aacgctgctg gagagcatgc gctgatgaaa catatccctg
     5161 aaggcgtgat tcaggcaccc gtatcacccg acgccccgga ggcgctgccg ggatgtcgtt
     5221 ccatttctga tgatgacctg tgcgcctggt gtacccgtct gctttaccgt cccggagaac
     5281 tcagtctgtg cagactgagt acaggcaatg gcatctggcc atccgtctgt gaccggaacg
     5341 gttaacggct gtccggaatt tcagccgaat atcattcatc cctgatattg ccgggtctca
     5401 cggtcagtga tgaacgtgat gttgtcagcc cccggccttt atttcctctc atttattcac
     5461 attacaagga tttcacacat gaaaacattg tctcagaaca ccacctcttc agcctgtgca
     5521 ccggagactg gcctgcaaca actggttgcc accatagtcc ctgatgaaca gcgcatcagc
     5581 ttctggccgc agcattttgg cctcattcca cagtgggtga ccctggagcc ccgtgttttc
     5641 ggctggatgg accgtctttg cgaagactac tgtggtggta tctggaatct gtataccctg
     5701 aacaacggcg gggcatttat ggcacccgaa ccggatgacg atgatgacga aacatgggta
     5761 ctgttcaatg ccatgaacgg taaccgcgct gaaatgagcc cggaagccgc cggcattgcc
     5821 gcctgtctga tgacgtacag tcatcacgcc tgtcgtacgg agtgttatgc catgacggtc
     5881 cattattacc ggctgcggga ttacgccctg cagcatccgg aatgcagcgc cattatgcgc
     5941 atcatcgact gagcaggaga agtacaatgc aacaactttc ctttctgccc ggcgcgatga
     6001 cgcccggcga gctcagcctc attctgcggg ccctgaaaac cctggaccgc catcttcatg
     6061 aacccggcgt ggccttcacc tccacccgag cggcacggga atggctgatt ctgaacatgg
     6121 cgggactgga gcgtgaagaa ttccgggtgc tgtatctgaa caaccagaat cagctgattg
     6181 ccggtgaaac cctctttacc ggcaccatca accgcacgga agtccatccc cgggaagtga
     6241 ttaaacgcgc cctgtaccac aatgccgctg ccgtggtgct ggcacacaat cacccgtccg
     6301 gtgaagtcac acccagtaag gcagaccggc ttatcaccga acgtctggta caggcgctgg
     6361 ccctggttga catccgggtg ccggaccatc tgatagtcgg tggcagccag gttttctcct
     6421 ttgcggaaca cggtctgctt taacctgtca ccgtcacaac cacatcatac ctgttttcgc
     6481 ttttatcttc tgtcttcaga ggtatcacac tataaaaatc gtcacccgtg gtgaagccat
     6541 gcgtattcac caacaacatc cgacatcccg tctttttccg ttctgcaccg gcaagtaccg
     6601 ctggcacggc agtgccgggg cgtataccgg tcgtgaagtg caggatattc ccggtgtgct
     6661 ggccgtgttt gctgaacgcc ataaggacag ttttggcccg tatgtccggc tgatgagcgt
     6721 caccctgaac tgaatcaggg cattctgatg agcagaatta tcgccaccac cggaccattc
     6781 ttaaccgatt ttttgtgagg actttatcgt gtcagacaca ctccccggga caacgcatcc
     6841 cgacgacaat cacgaccgcc cctggtgggg gctgccctgc accgtgacgc cctgtttcgg
     6901 ggcccgactg gtacaggagg gtaaccggtt gcattacctt gctgaccgcg ccggtatcag
     6961 aggccggttc agcgacgcgg atgcgtacca tctggaccag gcctttccgc tgctgatgaa
     7021 acaactggag ctcatgctca ccagcggtga actgaatccc cgccatcagc ataccgtgac
     7081 actgtatgcg aaggggctga cctgtgaagc cgacaccctc ggctcctgtg gttacgttta
     7141 tctggctgtt tatccgacgc ccgaaatgaa aaattaactc tccagaatag ccttctgcta
     7201 cggcccggtg ttttcaccac gccacttttc catttttata tctgcatatc aggaaaatct
     7261 tcagtatgaa aacattacct gtattacccg ggcaggcggc cagttctcgc ccgtctcctg
     7321 ttgaaatctg gcagacactg ctcacccgac tgctggacca gcactatggc cttacgctga
     7381 atgacacacc gttcgctgat gaacgtgtga ttgagcagca tattgaggcc ggcatttcac
     7441 tgtgcgatgc agtgaacttt cttgttgaaa aatacgcgct ggtgcgtacc gaccagccgg
     7501 aattcagtgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc cgggctcgcc
     7561 gggtgactgg cctgatgacc cgcgacaatt accgaacggt aaataacatt accctgggta
     7621 agtatcagga ggcgaaacga tgaaactggc cctgacgctg gaagctgaca gcgttaacgt
     7681 gcaggcactg aacatggggc gcattgtcgt tgacgtcgat ggtgttaatc tcactgaact
     7741 gattaataac gtcgctgaaa acggttattc actccgtgtg gtggaggaat ccgaccaaca
     7801 gtcaacctgc acacctccgc catttgcaac ccttgccggc atacgctgca gtaccgcaca
     7861 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacgttgg
     7921 tgaatcagaa tggattcatt tcacaggtag cggctatctg ttacgtaccg atgcgtggtc
     7981 atacccggtt ctgcggctta agcgcctggg cctgtcaaaa acgttccgcc gtctggttgt
     8041 cacacttacc cgacgttatg gcgtcagtct cattcatctg gatgccagtg ccggatgtct
     8101 gccgggttta cccactttcg actggtaacc agaaacaaca tgaaatcatt aaccacggaa
     8161 acagcgctgg atattctgat tgcatggctg caggacaata tcgactgcga atcgggaatt
     8221 atctttgaca acgatgaaga taaaacggat tcggcagcac tgttgccctg tattgaacag
     8281 gccagggagg atatccgtac cctgcgccag ctgcagcttc tgcaccagaa ccggtgaatc
     8341 tcactcacca gacttcattc cactgacgtc agcctgaaca gggctggcgt tttcgtttat
     8401 aaacccaaag ggaaaccgat aatgtcagac atcacaatct cccgtccgga actggtcacc
     8461 gggcatacgg acgttatctg ctcaaccagt atccgccaca ttctggctgt acgaaagagt
     8521 acactgctgc aaatcgacac acttatccgg caactggctg aaatatcagc aatgacagaa
     8581 agtattggcg gtaaaaccgc actggactgg gcaatgaaac aggattttcg ctgcggttgc
     8641 tggctgatgg aaaaacctga aacggcaatg aaagccatca cccgcaatct tgatcgagaa
     8701 atctggcgtg acctgatgca acgttcaggg atgctttcct taatggatgc acaggcccgt
     8761 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagtga agcgaacatt
     8821 ctgagcacat ttgaacaact gcaccagaat aagaatgagg tgtttgagcg aggggtgatc
     8881 aacgtcttca ggggactgag ctgggattac aaaaccaact caccctgtaa atttggcagt
     8941 aaaattatcg tcaacaatct ggtgaggtgg gacaggtggg gatttcatct gaacagcggg
     9001 caacaggccg atcgacttgc cgatctggaa agaatgctac acctgttcag cggcaaaccg
     9061 atccccgaca accgggaaaa catcaccatt catctggatg atcacatcca gtctgttcag
     9121 ggtaaagagg actatgaaga tgagatgttc agtatcagat actttaagaa gggctccgcg
     9181 catatcacgt tcagaaaacc agaactggtt gatcggctaa atgacatcat tgcgaagcat
     9241 tatccggggc aactatcagc catctgagtc agatggagaa aaagcttacc tgacccatta
     9301 cctgacccaa ttcaaaggca aaagaaaaag ggttagctga tttactagct aaccctt
//
LOCUS       DAZGTJ010000144         9236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000144 DAZGTJ010000000
VERSION     DAZGTJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.144"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1739
                     /gene="adeD"
                     /locus_tag="ACHK8E_004696"
     CDS             <1..1739
                     /gene="adeD"
                     /locus_tag="ACHK8E_004696"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP0346861.1"
                     /translation="HISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVI
                     KGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGL
                     TTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQ
                     MLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNTYIA
                     AGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDR
                     NPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIV
                     LLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQPVSASDFALQF
                     TPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGF
                     GLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNGQVQSHLPLPI
                     AGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDG
                     EKFAFTTLEVTE"
     gene            complement(1786..3177)
                     /gene="uhpT"
                     /locus_tag="ACHK8E_004697"
     CDS             complement(1786..3177)
                     /gene="uhpT"
                     /locus_tag="ACHK8E_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP0346862.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3433..4752)
                     /gene="uhpC"
                     /locus_tag="ACHK8E_004698"
     CDS             complement(3433..4752)
                     /gene="uhpC"
                     /locus_tag="ACHK8E_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0346863.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(4762..6264)
                     /gene="uhpB"
                     /locus_tag="ACHK8E_004699"
     CDS             complement(4762..6264)
                     /gene="uhpB"
                     /locus_tag="ACHK8E_004699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP0346864.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6264..6854)
                     /gene="uhpA"
                     /locus_tag="ACHK8E_004700"
     CDS             complement(6264..6854)
                     /gene="uhpA"
                     /locus_tag="ACHK8E_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP0346865.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6927..7217)
                     /gene="ilvN"
                     /locus_tag="ACHK8E_004701"
     CDS             complement(6927..7217)
                     /gene="ilvN"
                     /locus_tag="ACHK8E_004701"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0346866.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7221..8909)
                     /gene="ilvB"
                     /locus_tag="ACHK8E_004702"
     CDS             complement(7221..8909)
                     /gene="ilvB"
                     /locus_tag="ACHK8E_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP0346867.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9015..9113)
                     /gene="ivbL"
                     /locus_tag="ACHK8E_004703"
     CDS             complement(9015..9113)
                     /gene="ivbL"
                     /locus_tag="ACHK8E_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP0346868.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
BASE COUNT         2230 a         2652 c         2424 g         1930 t
ORIGIN      
        1 atcacattag ccgggctgaa taccaggaat tgttagccgt ttcccgtgga gacgctgttg
       61 ccgattatat tattgataat gtctctattc tcgacctgat caatggcgga gaaatttccg
      121 gcccaattgt gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatgctc
      181 cggctttgca gcggattgat gcccgcggcg caacggcggt gccagggttt attgatgcgc
      241 acctgcatat tgaatccagc atgatgacgc cggtcacctt tgaaaccgct accctgccgc
      301 gcggcctgac gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggcgaagccg
      361 gtttcgcctg gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca
      421 gctcttgcgt acccgccctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac
      481 agatgctcgc ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactatc
      541 ctggcgtaat tagcgggcag aatgcgctgc tcgataaact ggacgcattt cgccacctga
      601 cgctggacgg tcactgcccg ggtttgggtg gtaaagaact taacacctat attgctgcgg
      661 gtattgaaaa ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg
      721 gtatgtcgtt gatgatccgc gaagggtccg cagcccgcaa tctcaacgcg ctggcaccgt
      781 tgatcaacga atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg
      841 agatcgccca tgaaggacat atcgatgcct taattcgccg cctgatcgaa caacacaatg
      901 tgccgctgca tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga
      961 atcacctcgg cttactggca cccggtaagc aggccgatat cgtcctgttg agcgatgcgc
     1021 gtaaggtcac ggtgcaacag gtactggtga aaggcgagcc gattgatgcg caaaccttac
     1081 aggcggaaga gtcggcgaga ctggcacaat ccgccccgcc atatggcaat accatttccc
     1141 gccagccagt ttccgccagc gactttgccc tgcaatttac ccccggaaaa cgctatcgcg
     1201 ttattgaggt catccataac gaattgatta cccactcccg ctccagcgtc tacagcgaaa
     1261 atggttttga tcgcgatgat gtgtgcttta ttgccgtact tgagcgttac gggcaacggc
     1321 tggctccggc ctgtggtttg ctcggcggct ttggcctgaa tgaaggtgcg ctggcggcga
     1381 cggtcagcca tgacagccat aatattgtgg tgatcggtcg tagcgcagaa gagatggcgc
     1441 tggcggtcaa tcaggtgatt caggatggcg gcgggctgtg cgtgatccgt aacggtcagg
     1501 tacaaagtca tctaccgttg cccattgccg ggctaatgag caccgacacg gcgcagtcac
     1561 tggcggagca aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttaccggatg
     1621 agccgtttat tcagatggcg tttctttccc tgccagtgat ccccgcgctg aaactaacca
     1681 gtcaggggct gtttgatggc gagaagtttg ccttcactac actggaagtc acggaataat
     1741 aaaaaaaagc ccggcgtcat gccgggcaaa agtcaccagt taagtttatg ccactgtcaa
     1801 ctgctgaatt tttttctcgc ggcggatttt gcgttcttcc atcaccgcca ctatcgccat
     1861 caggcagata caaccaatcg cggcgatatc cagcgcggcg aaggtgcctg cccagccggt
     1921 aaggccaaat accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc
     1981 accaatcagg taagcgaagg tgcctttaat accatcggca gcgccaatcg cttttttagg
     2041 tacaaagcca acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa
     2101 cgcaaagaga gaagccaggt agatatattc gttgctggcg tgttgataca caccgagcgt
     2161 ggcgataatc agcgccagcg cgatacaggc caccaggcca cggcgaccgt tcgccaggtc
     2221 agagagccag ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagct
     2281 ctgaatcgcc accgctttag agagtttcag ttcctggaac gcatacacgg ttgaccactg
     2341 gtcgatacca atacgcacca catagaggaa aatgttggcg aagcacagca gccagatcac
     2401 tttgtttttc agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt
     2461 ctctttgtcc tcttcgctga tctcctcgcc gaacagctct tcagctttgc cgaggccata
     2521 agattccggg gagtcgctgc cgtaacgcag gccgataaaa ccgacaatca gcgcgataat
     2581 cgacgggaaa ataaacatgc cgatgacatg accatcgaac aggtaatttg cgccgaacag
     2641 cgccacacct gctgcgcctg caccaccaag gttgtgagaa atgttccaga aaccgaggaa
     2701 tgtcccgcgt ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc
     2761 ggtactctgg aaaaagccgc tcaaggcgta gaaggcaatc atcaggaaca ggctaaccga
     2821 gccgctgccc atactggcac tgaagcccag catacaaatg gcggacagga tcagcatgaa
     2881 cggcaggaat tgtttggtgt ttttgccgtc ggcgtagtaa gaaaccagcg ttttacccac
     2941 gccataagtg atggagaagc ccaggccgat catccccagc tgcgtcatgc tcaacccgta
     3001 ggtcgagatc atgtcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtaag
     3061 gtagccgata aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg
     3121 cacttcgagc ggaaggtcca gggtcggctt gcgaacctgg tttaggaaag ccagcatggg
     3181 ttactcctga tttcccttca tacttcaagc cgcaggtgcc ttagctgcac gcattcctcc
     3241 cggtcacata gttcactatg ctcccgggac tcacgcgttt gctgcgttcc tgcaacttga
     3301 attattttgg gaaagaatta atgaatgatg gcattgtaaa aatcagcagc aaatcaggcc
     3361 tgagatagcg tccaggcaaa acctggaaaa ttgcttagtt ttgccggata tgaagtgaaa
     3421 aggtgatatg catcacgctt cgcgcggtgt ctgggcgttc aaaaagggca gtaacagcag
     3481 tgcggaaacc ccggcggcaa tggcgataac cacaaaaaat ccgctccagt gccaggtatc
     3541 gagcactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa acaagccgac
     3601 aaaccccgtc gccgcccctg ccgcctcttt gtgggaacat tctgccgccg ccatgccgat
     3661 taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg cctgcatcac
     3721 gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc cggcggcgaa
     3781 aatcaaattc atcggcccgc ggttgccgtt aaacaatttg tccgagcccc aacccgctac
     3841 cagcgcaccg ataaatccac ccagttcaaa catcgtcact gccgtattcg ccgtgaccag
     3901 atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgattg ccgcccggac
     3961 cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca gtaacacata
     4021 tttggtgagg atctctttac gcgtcagccc tgccccttct tgttgttgag ctatctccag
     4081 cgcgtcgtgc cgccattcac caaccgccgg taaacctaac gcctgcgggc gatcgcgcag
     4141 ccgccagcag agaaaaatcc ccacgactat cgccatacaa cccgcaatca tcatcccggc
     4201 acgccagccg taatgcagcg cgctcgctgc catcacaatg ggaatgagtg cgccgccgac
     4261 gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgag aataccaggc
     4321 ggttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt tcaacaccca
     4381 aagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttga taatgcccgt
     4441 ggcaataagc cctatcccca taaaataacg ggcatttgag cgatcgctga caatgccgga
     4501 gacaaacttc gacacgccgt aggtaatgta aaacagggtc gctaacaggc caatatcgct
     4561 acggctgagc acgccgttag caaggatttc tggtacggcg gcgttaaaac ttttacgcgt
     4621 gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac gccagtagcg
     4681 atagcgggca tcaatttcat gtttatcagt cagtaatggc gcatcggcag gcgctttcag
     4741 aaacggcaac atcatcgaac cttagacata acgttgaggt agagaaacgc tgacacgcgt
     4801 gccgtgcaga caggaaatgt gtaatgtgcc acccagcgcc gttacgcgct cgcgcattcc
     4861 ggtaaggcca aaaccgtgtt gcccggaatc tggcggtaaa ccgctgccat cgtcttcaat
     4921 caccagcatt aaccgctcat catgctgcca gccttgcagc gtgaccgcgc tggcatcggc
     4981 atgtttcaca atgttgttca acccttcctg gcagacgcga aatagcgtca cgcgctggtt
     5041 ttcgcttaac gctgattcat cgattcgcca ttcgagatga ctgacaatgc cgcgcccttc
     5101 cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat catccagctg
     5161 gcgcggacgt aaccgcccta acaagcggcg caccgcgtcg taaacgccca gcgatagttg
     5221 ttcgatgagc tgcccgctct gtttcacgct ggcgttatcc gccgcgagcc gctggacaat
     5281 gcccgcttga gttcgaatag cagtaatggt ctgaccgata tcatcgtgca gctcacgtgc
     5341 cacatcacgg cgcacgctct cttcggtttc cagcaaccgt tcagcgagat gctgattgcg
     5401 cgccagttcc ttttgcagcg actggttaag ttcacgcaac cgctggatgc ctgcgcccag
     5461 taacaaccct gtcagacttt gcaccagcag cgagagcaat aaatccaccg gatgatcgcg
     5521 ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcaatta gcgccccttg
     5581 ccagccatag tgccaggcca gcgcgataat cggcagcgcc aggcagaatg gcgtaaagcg
     5641 cgacagttcg tccggcaatc ccaactggag ccagagactg ataacaaaca gcagcaagta
     5701 ccagaccaga tgccgcccac gccagttgat cggttgagaa actaacgacg ggccgagcgg
     5761 cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg ccagcgtcag
     5821 gccgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc aaagccaggg
     5881 cagcgactgc aacaacgccg ccgccgttaa tgccgcccct tgtagcagca aggtgcgcca
     5941 gtcacgttga tgtcgatagc gcgagatcag cgccacgggc agtaacgtca gtaaactacc
     6001 gatcatcaat aaggagaaat gggttaaacc gaccgcctgc gttaaccagt aaatcagcag
     6061 ccactccgcg cccagcaaca ccggccagta tccgcgcggg cattgcagca ttagccccag
     6121 acgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca ggtgcaggct
     6181 gatactccac aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa taacggtaat
     6241 taagcgggag aacaacgtct tcatcaccaa ccatcaaaca tgcggcgcgc cagttcaacg
     6301 tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac cgttttcggc
     6361 gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag tttttctgcc
     6421 acctggcgtt cgcgtttggt tagcgggtcc tgacgaccgg atgccagttt aatggcaata
     6481 tccggcgtca gataacagcc gcccgtggca accgtatgca ccgcagcaat cagttcgtca
     6541 gggctacaac gcttggagag aaagccgcgc gcccccgcgt taagcgcctg ctcaaccagc
     6601 gccggactgt catgaacaga gagcataatc gtcgccatac ctttcggcag ctggcttagc
     6661 agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg cacaccgcgc
     6721 cccggcaacc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac ctgcaaatca
     6781 ggttccagcc ccagcagctg cgcaaagccg gagcggacga taaggtgatc gtctataagg
     6841 gcaacggtga tcatgatctt gtcctggcgg gtaataaaac gcgcttacct taacgataag
     6901 cgcgatgttg ttcaagcctt gagcggttac tgaaaaaaca ccgcaatctt gttaaacatt
     6961 gtcggatcgg actgattacg ctgcactttc accacatctt ccagcttatc gatttggctt
     7021 atcatctgct ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg
     7081 tcctgaatcg gcaggcagag aatgccttca acgttaaaag cgcgacgggc aaaaaggcca
     7141 caaacgtggg tcattacgcc cggatggttg cgaacggtga gttcgagaat cacgttgtca
     7201 tgagttgtgt tttgcatggc ttattccccc accatttcag tattcgccgc acctggcggc
     7261 accatcgggt acactttttc ttcggcatca atgcgcacgt ggatcagcgc cggaccaggg
     7321 cgattgatga tttcctgcaa tgcagcctgc ggatcggttt cgttattcaa atcacaggtt
     7381 tcgaggccga atccggcggc aatctgcata aagttgattt ttcccggata ggtagcggca
     7441 aaaacgccct gcttgtagaa cagactctgt tgctgatgta ccagccccag cgcttcgttg
     7501 ttcatcagaa tgattttgac atccagctga ttttcgctag cggtcgccat ctcctgaata
     7561 ttcatcatca ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgcaagc
     7621 gctgcgccaa tcgccgcagg caggccaaag cccatcgtgc ccagcccacc ggaggtcagc
     7681 cactggcgtg ggcgattgag cggataagcc tgcgccgtcc acatctgatg ctgacccacg
     7741 tcggtggtga taatcgcgct gtcatcgaca caggcggcaa cagcgttgat caggccgtaa
     7801 tgcgttaacg gatcgcacgc tttcgggatt ggacacggga attcacgctg caaatccgcc
     7861 accaactggt gccactctgc acgcggttgc gcttccacca gcgggatcaa ctgcgccaac
     7921 acgtcatcaa catccgcctg aattgccaca tgcggttgct tgattttgcc cagctctgcg
     7981 cggtcgatat cgacatgaat aattttggca ttcgggcaga actgctcggt tttgccaatc
     8041 gcccggtcat caaaacgcgc gccgagaaca atcagcagat ccgcctcctg caaaatatag
     8101 ttggtgctgc gcacgccgtg catccctagc atacccagcg acagcggatg cgcttttggc
     8161 aacataccca gcgccattaa tgtcattgtg gtaggcagct gcgctttctc cgccagttca
     8221 cgcactctcg caggcgcatt gatcacacca ccgcccagat aaagcaccgg gcgtttggca
     8281 gcattaatca ttgccgctgc gtcacgaatg ctttcttcgc taaaggcggg cgcgatggct
     8341 ttttctgcca ccgcgggctg tgcttcaatc tcaaaaacgg ccgtttgcac atccttagga
     8401 atgtctatcc acaccgggcc agggcggcct gattgcgcaa tacggaaggc atcgctcatg
     8461 acctgcggga gttcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag
     8521 atgccgtagg tgtcgacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga
     8581 ccagtgatgc aaatcagcgg gatggagtcc agccgcgcat cggcaatggc ggtcaccagg
     8641 ttagtcgccc ccggtccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc
     8701 attccctgag cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc
     8761 gtgctttggc ttaaggcatc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca
     8821 atcttaatgc cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta
     8881 cgcgtcgatg ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc
     8941 cgggaggtgc ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg
     9001 tgttgttcca gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact
     9061 gcggcggatg gcgcagttgg tagtagtttt gcgttgagca tggaagtagt cattggggac
     9121 cttgttggtt ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg
     9181 aaaatttttc agcaagggaa aattgagggg ttgatcacgt tttgtactga attgca
//
LOCUS       DAZGTJ010000145         9215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000145 DAZGTJ010000000
VERSION     DAZGTJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.145"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..223)
                     /locus_tag="ACHK8E_004704"
     CDS             complement(<1..223)
                     /locus_tag="ACHK8E_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="type IV secretion system pilus assembly protein;
                     similar to F plasmid TraF; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFP0346869.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPG"
     gene            complement(216..473)
                     /locus_tag="ACHK8E_004705"
     CDS             complement(216..473)
                     /locus_tag="ACHK8E_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFP0346870.1"
                     /translation="MKVIFTSNRFVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(487..2391)
                     /gene="traN"
                     /locus_tag="ACHK8E_004706"
     CDS             complement(487..2391)
                     /gene="traN"
                     /locus_tag="ACHK8E_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFP0346871.1"
                     /translation="MKEKINTFFRSLNFNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSVHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGLVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGGTKTGVTEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGSCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDSGWGQDVGLA
                     ECNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(2485..2865)
                     /locus_tag="ACHK8E_004707"
     CDS             complement(2485..2865)
                     /locus_tag="ACHK8E_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346872.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(2862..3482)
                     /gene="trbC"
                     /locus_tag="ACHK8E_004708"
     CDS             complement(2862..3482)
                     /gene="trbC"
                     /locus_tag="ACHK8E_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFP0346873.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQLREKPDT
                     QLQAWAEHQVRANPLQHADNQFLDELVRKQQVAQKDKPQQGVVYFVSFSIPEEGLKRM
                     LGETRHYGIPATLRGMVNNDLKTTAGAVLSLVKDGATDGVQIDPTLFSQYGIRSVPAL
                     VVFCGQGHDIIRGNLRVRQALEKVAATGDCQQVAQGLLDGTGDKPQ"
     gene            complement(3711..4043)
                     /locus_tag="ACHK8E_004709"
     CDS             complement(3711..4043)
                     /locus_tag="ACHK8E_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346874.1"
                     /translation="MEKKLSSKYIHMYMSISCVFIRLTGKALKLFPFALVMLFTVLFY
                     FMPENVISDIISEWQRSDVSDRISVIRLYLGTTFLCTWAAVFLECLCSDEFLPGKRLN
                     KNEEENNG"
     gene            complement(4070..5062)
                     /gene="traU"
                     /locus_tag="ACHK8E_004710"
     CDS             complement(4070..5062)
                     /gene="traU"
                     /locus_tag="ACHK8E_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFP0346875.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(5059..5691)
                     /gene="traW"
                     /locus_tag="ACHK8E_004711"
     CDS             complement(5059..5691)
                     /gene="traW"
                     /locus_tag="ACHK8E_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFP0346876.1"
                     /translation="MKYRGLIALLIWGQSVSAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIARTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(5688..6074)
                     /gene="trbI"
                     /locus_tag="ACHK8E_004712"
     CDS             complement(5688..6074)
                     /gene="trbI"
                     /locus_tag="ACHK8E_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFP0346877.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(6071..8701)
                     /gene="traC"
                     /locus_tag="ACHK8E_004713"
     CDS             complement(6071..8701)
                     /gene="traC"
                     /locus_tag="ACHK8E_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFP0346878.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRHLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFRQLKEAGVVQRAESFNVVNLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERIRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGRYFNSDKPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(8832..9179)
                     /locus_tag="ACHK8E_004714"
     CDS             complement(8832..9179)
                     /locus_tag="ACHK8E_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346879.1"
                     /translation="MKSIYLKYFLSFVSVGVGAMFICGLLYNDYLGQQPATPEQLTEI
                     LQETPCAAEAFQVTLNYQSEPLTLGKANKIASECRKRNEMEEARRIRENERNKIREKQ
                     LQALSDADSAKER"
BASE COUNT         2164 a         2584 c         2149 g         2318 t
ORIGIN      
        1 ctccgggata cagaatggct tcgtacagcg cccgctttgt tgccgtctgc agcgcggtca
       61 gtttctgcat aatatccggc tcctgacggg gagctgctgg tactggttta ttttcttttt
      121 ccttcggatt tattttctcg ttataccact gccagcctgc atctttccct gacgccggaa
      181 aaataaagca gcagagtaac agtggcagta atgctttatt catcttcatt cctttcatca
      241 ctttttcttt catcaggttt ctgcaggtca gccagtgcag aaaaacaacc atacagaata
      301 aacaggagca caccggagta aaggataatg aaagcagcag caagcacccc gcctgccgtg
      361 gtttctttta ccgtatccca tgaccagata attaccgggg aaatgacaat tgctgtaata
      421 acaagccgga taagaaaatc aacaaaacga ttcgacgtga atatgacttt cattattttc
      481 ccgaccttac tgccccacct gtttcagctg accggcaatc tgctctttca ctttttcggt
      541 cagcgcacca ttatccggga ttttctggtt attcatcagg tcttcgtaga agttagtgaa
      601 gtccagctgg tcgaacttaa tacgctgcag ttcatccacg gttattcccc ggcagtcagg
      661 actttttgcg ctgccaaacc cgatacgcaa ctgcccgtta cgcccctgct gctggactat
      721 ctgcgccagc ttcgaatcaa actgacagta gctgcgcttt ttctgcaggc agacgcccag
      781 cactttccgg gagcagaact cacccacact caccgtcagt ttattgtcct tcgcctttcc
      841 cagcgccttt tcttcactgt tacattcagc cagtcccaca tcctgtcccc agccgctgtc
      901 cttacaacag ttactgaagc ctgcggcggc cttacggcag aatttcgcct cgcccgtgaa
      961 agccctgaca tccacaccat tcagggcggc aacatcctta cctgcggcgg caagcgcagc
     1021 cagctgtgat actgcctcac caaaatcact gttttttccg ctctgcgcct tatcgcattc
     1081 accgtcaagg cagaagacat caccaccaca gaccatcact tttcctgatg ttctggactc
     1141 gcaggagtac gtggcatact catgcagaca gcttccctca tcggaataaa acgcgcactg
     1201 acgtgaggcc agcgtacagg ccggattatc gacatatttc tgacaggtgc cattatctgc
     1261 cgactgcgtg acatatttat cccggtatgc ccagcacgcc tgtgtgatgc tccagggctt
     1321 accctccgtc acaccggttt tcgtcccccc cggctctgaa cactcagtgc ctttcagcac
     1381 gccatccgct ttattaaacg gacaactctc cacccactcc acccgtggaa cccattcctt
     1441 atcacggacc ttcatcctga gtttcagcgt aaaggtggaa gcaccactga caagcgattc
     1501 atagactttc ctgtcaccgt tcccgtgcgg gaggcaatta ccgtttgcag tacagccact
     1561 accggtcaga accagcccct gtgtgacaga aaacccggag ggcacaggta tggtgaaagt
     1621 cccgctctgt cctgtaacca gttgcacatt aaaggctgtg ttcataaagt cgtaacggga
     1681 gttaaggaaa tatagccctg catgaaccga cagcgaggca ctttccactg tcccggcaac
     1741 aggtacggta acgctgaaca ccagtttcag cccgttcata ctgaaccgga actgtgagtg
     1801 cggtatcgtc acttcacgat attcatccgt gtacttccag tctccggtga tactggcagt
     1861 ccgggagcag tattcctcca ccatcgtgtc acgttcacag gtatagtttg tgaattcact
     1921 gcggtttatc tgctgcgcac tgcactgctg tccggtattc cccacgatac tgtcagcccg
     1981 gttcaccaca tccctgccgg tctgaataaa cggcgcatcc ggtgacagaa tgtctttcgg
     2041 cttgttcatg aatgactctg tgatggtttt gccggtttca ctggttgccc attgtgtggt
     2101 gccgttattt ttcagaccgc catccccgcc ggcagtcacc ccaccgtaat actgcgtctc
     2161 atccgggttc gcgttatacc cggggatact ctcctgcgga ttaaaactct gaatactgcc
     2221 aataccctgc ccctggatct gacgggcaaa atcggaacct gcccggtaat cactgttgct
     2281 gtcggcaaac acagaaaagc tcaggaaaaa aacaattgca caaatattgc gcggtcttcc
     2341 ggagtcatta aaattcagag acctgaaaaa ggtatttatc ttttctttca taacctgatc
     2401 catactggag aatgaagtga tgtcatctca acttcagaac ggtgttgatt ttggtgtatg
     2461 gagcttcctg gcgtaccggg attactactc agataacgac ttcagtaaca atatcagcgt
     2521 cctgaagtct gcacgcagtg aacgacgaca gtaccggtga taccagcaat gaggaccttc
     2581 cggctgaagc cggaaacaga gctgaaggtc actgttaaaa gcccccttca ccgaccatcc
     2641 ccatgtcaga cgtggtattg ctgattcagc atcctgccgc agtcgctgct caagaagggc
     2701 agccattttt accggacagg caaaggcaat ataaggcagc agctttgtaa ccgggaaacc
     2761 atgtccctga caggaggcat atgtacggaa tccgtgaacg ttcatcgcgg ccaccagtgc
     2821 acggatgtcc gggtcaatcc ggtaataccc ggcaggtaaa ctcattgcgg cttatccccg
     2881 gtgccgtcca gcagtccctg agccacctgc tggcagtctc ccgtggcggc cactttttcc
     2941 agcgcctgcc tgacgcgcag atttccccgg atgatgtcgt gcccctggcc gcagaacacc
     3001 accagggccg gcacgctgcg gatgccgtac tgtgaaaaca acgtcgggtc gatctgaaca
     3061 ccatcggtcg cgccatcttt taccagagac aacacggctc cagcggtagt cttcaggtca
     3121 ttgttcacca tgccccgcag tgtggccgga ataccgtagt gccgggtttc gcccagcatc
     3181 cgtttcagtc cctcttcggg aatggagaac gacacaaaat acacgacccc ctgctgtggt
     3241 ttgtctttct gagcaacctg ctgtttacgg accagttcat cgagaaactg gttatcggca
     3301 tgctgaaggg gattcgccct gacctggtgt tctgcccatg cctgcagttg tgtgtccggc
     3361 ttttcacgca actgctgctt caggaactgg cggttttcgg gcgtattcac gttttccgaa
     3421 gcatgggcag aaagaaacaa tcctgctgtc agggcgaata aaatgtatat ttttttgttc
     3481 atagaattct cctgcaaaca gacagtaatt aatcatcata atgaggcgct caatagagcg
     3541 agtctggaga taactgacgc catgttaaac acaacgaaga tcgttgatta acttctttct
     3601 ccatttatct tttttctgag actggcagca attagataaa cgataccgga tattcactct
     3661 gttaaatcat taattcattt ctgaccgatg cacataaaat aagtcagata ttacccgtta
     3721 ttctcttctt catttttgtt taatctcttt cccggcagaa actcatcaga gcagagacat
     3781 tccaaaaaaa cagccgccca ggtgcataaa aaagttgttc ccagatatag cctgataacg
     3841 ctgatcctgt ctgacacatc acttctctgc cattcactga taatgtcact gataacattt
     3901 tcaggcataa aataaaacaa caccgtgaat agcatcacca gtgcaaaagg aaacaatttt
     3961 aatgctttgc ctgtcaaacg aatgaacaca caggatatac tcatatacat atgaatgtat
     4021 ttactgctta atttcttttc catttaaaat attcctcttt ttgtttaagt tacagaaata
     4081 cacagttacg tttacgccac atcaggtagc cgaagttttt ctttgtgttg ggcggatttt
     4141 ttcccgtctc ccagcgcatc acactgcgtc cgaacggatg gcactgtccg ctgtccgggt
     4201 acatattcac catctgataa cgccagcgct ctttgggaag tatgggcgac ggatattcat
     4261 aacagacagc cacatccacg ccgatgctgt tcattatcat tccctgacgg tgcaacttga
     4321 acgccatccg ttcactgacc agcagggatg actgcaacgg actggattca ttgctcaccc
     4381 agccattaaa cggatacata ctgccctgcg aaccggcaca ccagaacagg atatccagcg
     4441 gcttgttaaa ggcactggca atggcatccg ccgcacaggc tccctgtgct atcggatttg
     4501 caaagatgac cgcttccggg ttgagaatgg tggtcagact gctgtctgtc caggtagggt
     4561 caatttctga gatgtacccg atatccatat cgccgcccat cagacatcct gctgaggtga
     4621 taatactcag ccagtatgtc agcgggtatt tgtaccagtg aacatggtaa aatgccccgt
     4681 tcacctgctt gtcgtctttt ttggccgttc cctgaccggt tttccccagc tccgggattg
     4741 ttattccaag atttaccata catcccggac tgcgggtcac atccgtcagt gccatcggct
     4801 cccagtaacc aatagccagc ccgatacgtt taaatacagg cggtggcgca ggacagaact
     4861 ggatcggcat tgacgggttt attgtatccg gcaccttccc ctgactcact tttgtgctgc
     4921 ccagcgacag tggaaaaatg cagttccagc agatatccgt gatcgggtta acaaaccgcc
     4981 cttcacaggc agcgtccgct gacgctgcgg gaacatggcc agtcagcagc aaaaccacac
     5041 acaaccacat acaccgtttc atttccctcc ctccccgacc ggaataaact ccaccttcag
     5101 gaaacgatcg ccactgacag cactcacccg tgccggcacc tgatcaatgc cgagacgctg
     5161 gcagatcacg ccgttctgat caaagtaaac acgactgtcc agagccttct gcatctccgg
     5221 gatactgccc tgcaccagga taattttgct ctccagtgtg ggaggcgtct ggcgcttcat
     5281 ccattcaatc tgctcaggat catcaccgtt gatgaaatac agcgtctggt tgaaaggcac
     5341 atactgcagc gggttcatca cctccccctg gcgggcgaag atacgacctt cgttatcccg
     5401 gatatccgca gccagtctga cagacgggtc aaacagccgg ctgctgtatt tctccgtccg
     5461 ggcgataccg ggcacggcag gaggccgcag gctgttgcgg atcacccgct ccttaaacgc
     5521 atccattttc cggcccatct caccggactg ctccagtgcc gtcaggcgct gcatgatcac
     5581 cgtcagcatg tccggctcct ttaccggcca caaatccccc caggtgccaa gatcggctgc
     5641 ggacacactc tgcccccata tcagcagggc aatcagcccc cggtatttca tggttctgcc
     5701 ctcattcgcc gggcaatatc ctgctggatt tcacgggtga tatccggtgc cccctgtacc
     5761 acggctggcg acaccagaat gacagcgtgg tgcttctgct gccacgtctg cagactggcc
     5821 tccagtgccg cgttaaaacg gtcagccagc gccttcgact gcgcttctga cagttttttc
     5881 tggctggcgc tgtcaaaaaa cgcatccact gtctgtttca tgttgaaggc gacggttacc
     5941 ggcgtgttca gtctgacaat gccgtaactg acggctgcgt tcagtaacac cattgccagg
     6001 catcccggca ccgtccacca ccagtggctg cgtcgacggg ctgtcacatc agtcgttttt
     6061 tgcgttgtgg tcatgcaaca ctcctgtatt tctcatgttc ttccagccag gcctccagcg
     6121 aagccatctc cgggcctgac ttcttccacg ccagctgcca caccgcctca tgaatgctca
     6181 gcccctcttt acgtttctgc tgcacaaact caaaatccgg gccgtcagag ctgtacatgg
     6241 cacggcttag cgggtccaca aacagacggt gccaggagga atggttttcc acctgcagca
     6301 ggaaggaact gaaccactgg tctttggccg caccaaactt gccaatcatg tcacgctgca
     6361 gtggcaggaa ctggtccggg aataactggt tatatttcgc gaactccttc gcactctgct
     6421 tgaggataat tttgtaggag gagttacccc atgcagcacg ggccgcactg gaggccttgt
     6481 cagagtcaaa gtcgacgatg ttctgtgtaa tggtgatata ggcaccggta tgacggcggg
     6541 cagtacggta gcctttttga ataaactcac cgactttacg atttttgaag tccagcaggc
     6601 gccagccttc atcaatgacg ttcagttttt tgagagtacg cggcgtgcgg tacatcctgt
     6661 tctcgatata gataatcagg gagaacatca ccgcaaccag cagtgacgga cggtcttcca
     6721 gtccacccag ctccagaaca accattttcg catcatcccg cagggacggc ttatcagagt
     6781 taaaataccg gccgtaggtg ccgttggcag tgtactggtc aagcagcaca atcatttcat
     6841 ccaggcggct gcggatggtc ggtgactcgg catactgctc gctgtcactg gcgtttttca
     6901 ggaaatccac cacgtcatca atacgtgccc tgttctcttt ggccagccat gatgccctga
     6961 ccgcctgcag cagcagacct tcatgcactt catccaggtt accgttgggg ctggccatca
     7021 ccgacagctg gtcacggata cgctccgctg actggtcaat atcggtgatg ttagcaaacg
     7081 ggttaaagcg cagggtttca ccgtccaggt atacgccccc catgttttca cacagggact
     7141 tgtagccatc ccccatgtcg aacaccacgg caaagccccc ggagtccagc acgctgcgga
     7201 taagtggctg tatcagcccg gttttaccgg ccccggaagt gccacagacc gccatgttgt
     7261 agttggtgtt attcatcccc ttaaagaaga tatcgataaa cgccagctgg ttacggtagg
     7321 tgggtgccag cagacctgcc ggtgtcaggg ggttatctgc cactaacggc atcaggttga
     7381 ccacattaaa gctctctgca cgctgtacca cgccggcctc tttcagctgt ctgaacagcc
     7441 ctttcccggc cataaagggc aggcaggtca ggaaattgcg catgtggtta aagcgcggtg
     7501 aaatcagctc aaaaccgttt ttacggaagc tgttcaggat gtcctgctcc acttccagcg
     7561 ccgtttcatt gttgtccttg cagaacgctg tgatgttgag gaagtaggac accacggagg
     7621 actgacctga gcccagccgc tgacgcagtt ccccccactc cttcgcttct ttctccacgg
     7681 acggaaacca tttggcatag gaggtcttcg acttcttctc caggtccatg tacttcaggt
     7741 tggcttcgct gtgggttttc acctggtctt ccaccaccag ggtcagcgtc aggatgaacg
     7801 ggcaggaaat ggacatttcc gggttcagca ggttgctgta gttgtcggcc atgttccaca
     7861 ggaaggcgat ttccgggtta cgggccagat ggaaattcag aatacgggcc gtactgttcc
     7921 tgccgttctc acgcagcccc agcgtcaggt aatccgcccg gaccttgagg tcaaaactgt
     7981 cctccacaca ctgataattc agatcagaat acgggtccag atgacgtctt ttcgggtaca
     8041 gggagtccgg attatggtta atcatctccc cgacaatatc gataaaggcc tgtgcatcca
     8101 ctgtctgtgt ggtgattttt gcgccgtgaa aagaggcacg aatgattttc accagatttt
     8161 ccatttccag aatgtctgcc cggctttttt tctttgaagg agaacagtac gagatgaaca
     8221 cccggtagtg acgcagggtc agcggcagat tcatcccctc cggcagcgga aacagtgttt
     8281 ctgcggcttt catgtaatag gcccgggtaa tggcgttaaa ccgttctgcc tgttcacctg
     8341 accaggagaa ctcacgcagc ccgtattcaa tcctgtcacc aaccagctga ctggacatca
     8401 gatggataca cagcggaata ccgcgcggta gtttggtgcg cagcatatga tcgagcgcct
     8461 ccacaatggt ctcattcgca ccattgatgg gaatagcttc cagcataaag cccattgtgg
     8521 agtcattcat gaacagaccg gattcctggt tgtaatcacg gtatggcagc agacggctga
     8581 actgcgggaa gttcatctca ccgaggatct gattagcctg agcagattca tccggtaatt
     8641 taagtgcggt cagcagtgaa ttgaccgcct gagtgacgac ctcaagtggg ttattactca
     8701 tatgagtatt ccttagtacg ttttaaaaag gaaaatatca gtctgtggcg tggatatatc
     8761 acgcagtgcc attcttaaaa ccacagcaca aataagagat ggaggctttt aacaggaaac
     8821 caggattaca attaacgttc ctttgctgaa tcagcatcac ttaatgcctg aagttgtttt
     8881 tcgcgtattt tattgcgttc attttctctt atccgtcgcg cctcttccat ttcatttctt
     8941 tttctgcatt ctgatgcaat tttatttgcc ttaccaaggg ttaaaggttc agactgataa
     9001 ttaagtgtca cctgaaatgc ttctgctgca cagggagttt cctgtaaaat ctctgtcagt
     9061 tgttctggtg ttgcaggttg ttgcccaaga taatcattgt aaagaagacc acatataaac
     9121 atagccccaa caccaactga tacaaaagac agaaaatatt ttaaatatat cgacttcatt
     9181 tcattttcct tcgaagcagt aagcactcat agccc
//
LOCUS       DAZGTJ010000146         9179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000146 DAZGTJ010000000
VERSION     DAZGTJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.146"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(30..455)
                     /locus_tag="ACHK8E_004715"
     CDS             complement(30..455)
                     /locus_tag="ACHK8E_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346880.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKYEGAFMIYKDSRGELDYPFTISLSG
                     REKKFGKNDLIRLSRDISTKDDRKYGVIRYIIDSQEICP"
     gene            complement(448..1854)
                     /locus_tag="ACHK8E_004716"
     CDS             complement(448..1854)
                     /locus_tag="ACHK8E_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0346881.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRTFDLISSLDPDMQSSVSLLGFEKIYHNYGGYYNNSKDEIKALLYSNERYKWDSAH
                     EMGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDIMKYADSYAPDRYKRLVA
                     ADEDVQALIWLSRVSFND"
     gene            complement(1860..3470)
                     /gene="tssA"
                     /locus_tag="ACHK8E_004717"
     CDS             complement(1860..3470)
                     /gene="tssA"
                     /locus_tag="ACHK8E_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0346882.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            complement(3463..6789)
                     /locus_tag="ACHK8E_004718"
     CDS             complement(3463..6789)
                     /locus_tag="ACHK8E_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP0346883.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(6846..8060)
                     /locus_tag="ACHK8E_004719"
     CDS             complement(6846..8060)
                     /locus_tag="ACHK8E_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346884.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            complement(8166..8555)
                     /locus_tag="ACHK8E_004720"
     CDS             complement(8166..8555)
                     /locus_tag="ACHK8E_004720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346885.1"
                     /translation="MLINFFFCSNSYSEEKIEIININKPIYIGTNQSDKVLITACNHW
                     VLNSRDIIDIFNLSIKHDDYGEINRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWM
                     SNDKEIYWGCNKKACDNFFLLPYDGMR"
     gene            complement(8545..>9179)
                     /locus_tag="ACHK8E_004721"
     CDS             complement(8545..>9179)
                     /locus_tag="ACHK8E_004721"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP0346886.1"
                     /translation="LSEEKKEDEKCTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPK
                     INAIILHRTDSSTINGTLASFSKGIGTHFIVEKDGNIYQAASLFVYTSHVGKIKSRCK
                     EEKTWSSEEKKKTEAFGWNAKKIHDHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKW
                     ESATDSQKKSIEKLVDIIKNIYELEDRDVYEHDKISYKTEGEGADLYSVN"
BASE COUNT         2035 a         2587 c         2219 g         2338 t
ORIGIN      
        1 taataattgt gaacgaataa cttaaaacat cacgggcaaa tttcctgact gtcgattata
       61 tatcttataa ctccgtattt tctgtcgtct tttgttgaaa tatctcgtga taatctaatt
      121 aagtcattct tcccaaattt tttttcgcgc cctgacaatg aaatggtgaa tggataatcc
      181 aattcaccgc ggctatcttt atatatcatg aatgccccct catatttaaa accatcatca
      241 atatctgccg tggaaaaata tactaataca tcactatcac caactggcaa gtgtatatta
      301 tgctgataaa tcagcgtaga accggaaaca tatccctgaa taaagacatc gatatctgag
      361 gagcatctat tctcaatcct gactatattg gaatactctt tttgtgaagt acacccactc
      421 aaaaaaaaca ataaaaaaac aattattcta atcattaaaa ctcaccctgc tcaaccagat
      481 caatgcctga acatcttcat ctgccgcgac caatctctta tatctatcag gtgcatagct
      541 atctgcatat ttcattatat caatctctcc tgaagatggc attggagtat tagggatggt
      601 actttgaccc cgcgtggatg tccccttatg tgtccatgat tcagaaccat atttatccat
      661 aactttatgt cccatctcat gagcactatc ccatttatat ctttcattac tatatagaag
      721 agcctttatt tcatccttgc tgttattata atagccacca tagttatggt atattttttc
      781 aaaccccaaa agacttaccg acgactgcat atctggatct aacgatgata tcaaatcaaa
      841 agttcgcata gatggagtaa tatttatctc agcctttaca tttactttaa aaaccccaat
      901 cgctgtaacg atattaccac caattccccc gccgcgactt ccatttcgtg accagtaaaa
      961 ctccacgcca tttttagtaa gactaactaa atcgctataa ggtattggcg ataactcagg
     1021 gttactgcct tttgtaccgc tatccttaac agataatctc catgttatat cgaccacttt
     1081 attatttctg tcaactttta catcaaccca ctcttcttct ttcgcagcat ttttcaatgg
     1141 ataatcaact tttatcatca tatcaccaca taaagcaatc agtcttacga gtagtgattt
     1201 gctttttatc ttagttgtat ccagtatgcc ttgattactg tagccatccc attgccaggc
     1261 atgctctccc ttatcacaat atttaccggt ctgccgttca ctataaatca cttcattacc
     1321 atcaagcact tccagagaaa gcgcttcagc ctcatcgcct ttatttgtga tggtaaaatc
     1381 tattttttct gtattcttcg tactaccacc gctaatcatt tctttataat caggtatatt
     1441 cagtggcgtc agtatcttat tacctgataa tttaattctg taatccagtt ttacatcatc
     1501 agaggatgaa tcagaaccag aagtcagatc ctttttcgcc gactgcgcat gttgttccgc
     1561 ttcactttcc ccgtcatccg tctcatacgt atcatccatc attgacgcaa taaaccgtgc
     1621 cttacacggg caactgctct gactgtccag cgtccccgcc atcagcctgc cgtgaatatc
     1681 tgtttcagga acgccgccca caataataaa cattcccgga tatctgccgc aggtgacccg
     1741 gtccatatcc cgcgcctgcg ggatacccat gatcgtatgg tcgtccgcgc cttcaataat
     1801 tttgccgccg caggtggttt tgtcccccct gaccagaaag tagcctgttc ccattgccat
     1861 cagtccttat gttgcgtatc acacaatacc gctgcccgta ccggatctat tgtcaccagc
     1921 gttcccagca ggatttccat ttttcggttc agcagggctt tatccgcata gcggtgcgcc
     1981 cgcaggcgca gcagtttcag ctgtcgggct tttacctcaa acagcaggtc cggctcccac
     2041 tgcgtcagcg tcagcccctg tgcggcgata tccagttctt ccagcagcag gagcgccatc
     2101 tcgtttttac cgtactgttc cgccacccgc gccatcagca gtcgcaataa caggcgctgg
     2161 cgtgccgtgg tgatgcccgg acgggtctgt atccagccca gcgccgcttc cacgccctga
     2221 ctgtctgcca tctccagcgc ttccggctcc agcgccagta cgtcgtttgt ggcgtcgtct
     2281 gtcggtacag cggctttccc cgcaagccat gcgccgtctt cccgcatcat gacctgctgc
     2341 gctatccagt tgcgcgtcaa ctcatcggcg aacggcgtgc cgtcgttcca tgccagattt
     2401 tccagccccg gcaggcgttc cagtagtaac gccaggtcct gacggataac cgctgtccac
     2461 ctgtcccacg gtgcgcccgc cttagtcagc gcctgatgca gataccactg aatgtccagc
     2521 cagaaatggc tgacgccggt gctgaacatc aggtcggcct gctccagcag ttccgtccag
     2581 ttctgctgag catacagccg cttaagctgg ttgcgtgatt ctgtgcgcgg cggggccagc
     2641 cgggtttttc cgtccacatc cggcggcagt tcatgcaccg tgtcccagcg cagggttttc
     2701 atcagccggt gtgctgaaag ccagccctgc ggctgttcgt tcagataacg ggccagcact
     2761 ttcgcctggt cgagcaggtc gcgcccggaa gtgatggtct gtacctgtgg cgcatccacc
     2821 ggggaagacg gtgccggaac gccgctgctg ttctgcggca ccaccgcgtt catcctaccc
     2881 gactgcgcca gacgtgattc cagcgcgtta atcagcggca tcagggaggg ggaatgctgg
     2941 tcttcgggcc atgcagtgaa ggaaaccgtc agttgattaa gagcggcaac aatgttcgca
     3001 aaatcctctt ttgccacttc cgggtagcgg gcaaggctgt cgagcatctt ttcccctgcc
     3061 agccactcca gcgccatttt tcggcttgcc gggcgcgagg gcagcagttg cgcaccgaag
     3121 cgttccacca gtccggctaa cagcgccagc ccctcagcca gtccgcgttc gccatcgcgg
     3181 tgcagtcgcg cccagatata gtaagtggcg atgcgcacgt cttttgcctg cgtcagcagc
     3241 agggattcgg caagctggca gataaggtcc gtatccgcgc cggaaagttt gttcatctcg
     3301 tcgcggatac gcaggaaatc gtcgtggtag ccggggtctt ccccgaccgg cgtatctgcc
     3361 gtcaccggca gcagccatgt ctgccacagc gccgcctgtg ttcgcgccga gtccagtgcg
     3421 tcccggtcgg caaaacagct acgggtcagc ttttgcaaca gttcactcat cggcgtcttc
     3481 cccgtattct tccgcatcct tcatactcct gccggtcagg aacacctgtc ccggcaggcg
     3541 gaagtttttc agttccagca gcgccagcgg ccctttaccc tgttcaaccc gcagcaggta
     3601 attcagcggc aggccgtcct gcgctttcca caccacttta aaggtactgc tgtccaccgg
     3661 tgtcacctgc gcctgttcca gcaggcgaat aaggctccag cttccccgat aatccgccag
     3721 tatgcgttcc atcgcctgcg tactggtcca gctcagcgat gcgccgggtt gccactgttc
     3781 atccggccag ttaaaacgct gccagcgttc cttctggttg aaatattcca gctcctgccc
     3841 gtcaatcacc agatgcgtct tcatcacgtc gcgcgccggt tttgcccgca gttcaaaatg
     3901 tatccctgca tcgccgctgg caaaggcggt atcggccaca tcgcgcaggc ggttcagggc
     3961 gcgaataaag tccggattga ctgtcagccc ctgcgtgttc atggcgtcag ccacccagta
     4021 gcggccttca cgtttcagta cgccggaaag attggcggcg ataaactggt tgatgcgccc
     4081 gtcatcgcgc aggtactgcg ccagcagggg gagggaggca tcgttttgtg aggctttgaa
     4141 cgggtagcga ccagcgaagt cctgatccca gtggctgaca atcgcccgct gccactggcg
     4201 gttcaggctg tctgccgcag gcgtcagcac ctgccgccat gactgctcca ccgggcggac
     4261 gaacagcgcc tgaccgaagc cactccactc ttcacccaga ctggcggcta tcaggctgcc
     4321 atagtcgcgg gtgtcggtaa ggtcaacggt tttgccttgt aagaccgtct gcgccagttg
     4381 ctgggtcatc tcctgcgggt caggggcgtt ggtcacctgt tgcagtttga ggcgcacgcg
     4441 ggtgactcgg gtgaggtagg tctgcaggct cagctggctg tcgccgcctc ctgccttatc
     4501 gccaagcaac cgcagcagcg gcgcgaaggt tttatccagc ggcccgacag gcacgtcgcc
     4561 ttcacgggat tgctgcggcg ttttctcctt gccgttaaac agttcctgtg ccgatttcgc
     4621 cagcgagtct gacaggccac ggttttccct gccagccgcc gcctgccacg cgagggtgtc
     4681 tgtcagcgca atcagcggtg actggcgggc atcggccatc agcgtcaact ggtcgagaat
     4741 gccggagagc gagtcttcct ttttccagcg aatgctgttg agaaacgcca gccagcttcc
     4801 ggcaaagtcg gtaaagtagc gtgaagtgag acggttacgc agcatatccg gcgagatatc
     4861 cgcagaggta tcctgctggc ggtcgctgag tacccagtcg atttcctcgc gccgcgccgt
     4921 caccacctgc tcgatggctt ccctgacctg tccttcccac gcctgacggg taaacatccc
     4981 cggcaccgtc tgctccgtac tgaaaagaga ttcggtgagc gtatccccgg tcatgtccgc
     5041 cagcgtcata tcggcgtagt tgcgggacac ctgttgcagt acgttctggt agagagtgtt
     5101 ttcggcattg cgcacaccaa tctggcgcag caggattttg cgcactgcgc cggtcagttc
     5161 cggtggcggc gaagttttcc actgtgggtg ttccggcaga ttcgccatcc agaaggtcag
     5221 cagtgacggc aacacgctct gacgcacacc ttccggtaca cctgcatagc gcaggtcatc
     5281 tgccatcagc gtggtactga aaaaggcagc gtcggccttt tccgggcggg aagtcatcag
     5341 caatgcctta agctgcttat agcgctgttc gccggtggcg gtacgctgcg gactattggg
     5401 cggcagcgcg acaaaggcat tcagttgctg ttgcagatgg tcaacggcca cgtcgcgcac
     5461 cagccggttt gccgcctgcg catagccggg aaaggcggcg gcgagcagtt gctggttacg
     5521 ttcaaggcca aaacgctgat accacggcgc accctcccgg atacggtact gcaggcgctc
     5581 cagttcaccc tgcagggtat gcagcgccac cagttgttca gacagcggca gacggatatc
     5641 gagtgcacgc gccgtctgga tgctggtttc ctgaacaagt gcgcggttgg cgaagaacga
     5701 ggtcgtcatt ccggcccccc atatcgccag tacgcagaca gcagacatca gcgtgatacg
     5761 cagccacgga aagcccaccg tgcgaccccg cgcgttatcg ccggtcacgc ccgcccagac
     5821 cggtgacggc agccacgcat gaggtgcggc ttccggtacg gcggtaagcg gcgggctgaa
     5881 catcaggcca cgcaggcgca gggaggaaaa tgcgccgcct gccagcagcg gtttcaggat
     5941 actttgccag tgagcgatac cccgctctgc aagggtatgg gccagacgca gcaggaaatc
     6001 atggcggtta tcggtaagca gctgcgacat gccctgttcc gtcagcggca gggtttgtgc
     6061 ttccagcatt gcagccagtt gttccggggt acagcgttcc gacagcaggc agccgacgct
     6121 ttgcaggggg cgtccggtct gataatcacc gttgtcacag acctgccaca aatagagtgg
     6181 agcctgaaaa ccgagtcgct taccgccgtt tatcagttcg cgccagcctt tgtcgagttg
     6241 cgccgtctgg cggctctgtt cttctgtcag cggccagatg atgccgtcca gcggacggct
     6301 gcggcgcagt ttttttaagg cggtcagtag cgcgacatca ggctctgtag atggcctgcc
     6361 gccatatatc agcaccgtgt ggtcgccctc cagccagtgt tgcccggtca gccccggcgc
     6421 gatggcctct gcctgttcag gctcgccggt caccagcagc aggcggattt tacgccgcca
     6481 gaatgggccg tagcggtcgc gcaggaaggc ggcgagttcc accattgccg gatgacggct
     6541 gtcggctttc tttgtcgtca ccgcccgata gtcatctccg gcggcaagct gtctgtggag
     6601 ttcaaagctc tgtacggcag cgtggcggtt cagcagatga taaaccccca tgataatcag
     6661 aaagaccaca cctgcggcaa tcacgccagc catccagtac atttgctgtg aactacccgg
     6721 ttcaacgccc agcaatgccg gtttacggtg aataaacgtg caaagcgcaa ccacaacggc
     6781 cacggtcatg acgatggcag gccctgaaac cgaacggggc cggagacggt ttaatcccgg
     6841 aaccactatg atgcctcctg aggggaaacg aatggcgaca ccatggtcgc ccatgaataa
     6901 tgcccgttct gaacgccgct gagcgtcagt tgggggtgtt gtgattgtat tgccgccagc
     6961 gttgccgcag cgacggacag ccacggcgct gccggtccgg agcaccccag aaaggcatcc
     7021 agctcgtaca tcagggcttc ctgagtaaac ggctggttag atgacagcag cgaggcgcgc
     7081 tgttccctgt tcagttcgac tgtccacatg ccggatatgg catctggctg caccggcatc
     7141 cagtccagcg cctgtgcaat gcctgacgcc atcatctccg tatctgtgat acgttcagga
     7201 cgatgcagca gggccagcgg cgtcagcgcg gtttgcgtca gtcggtttgc cagcaacagc
     7261 gcggtggcgg attctgccgt ccgttccggg ttcttcggtt ccagacgcac ggaaatcacc
     7321 agcagtacgg ccttttcctg aataaagttg tccagccagt cgtccacggc ggccagcccc
     7381 ggctccgggg cagaaaacag acgggcttcg gggagtccgg ctttttgcca ttcgtcctgc
     7441 cagatagttc tcacggaggc ccctgaaagc ggcgcatcaa tttccagtag tacgttgata
     7501 tgaaaatcag ggggcagcag aaacaattgc ggtctgatat caataaaaag gcgggaaata
     7561 atttcggata aacgcccggt aaccggttta ttggttaata cgggaatgac actgcgacgc
     7621 acggtatctt ctccgcgcaa atcagcgtaa gtattcaaca cctgatgacg cgccagaaat
     7681 gctgttgtag tttctgcgac ggaatcacca ttcagcgcgg tttgtacgga aaaactgagt
     7741 atctgcaacg cccgtcgtcc ccgccgtgtt tcctgcaaaa tgacttcttc gcgacgttga
     7801 ttccaggcat cggcacgaat atgttgagtg acaaatacca gccagcgcac cgaaccgacc
     7861 agtgaccaga taaaagcagg cacgccgcag gccagccccc agaaatatgg gccgtttttt
     7921 gccagttcat cgctggcgaa taatgaggtt attgccacac caacagacag catcagtaat
     7981 aaggctgaca gccatttaat gcgggctggc ggtgcgggat agggttctgc gtcgggaatg
     8041 gtattaagtc ggatgggcat tgtaataatc cgttattctg aaatatgaat acaatgaatc
     8101 tcaaggtgct tgaactatct gattaattca cttgtccata atgtttttgc tttattaact
     8161 gtgatttacc tcatcccatc atatggtaat aaaaagaaat tatcgcatgc ttttttattg
     8221 catccccagt aaatctcttt atcattgctc atccacattg ctgttgcagc agcattgata
     8281 acaaaactcc attctattcc gtcatacatt agtctccctc taatttcaca aggtaaccaa
     8341 taatacattc tgtttatttc gccataatca tcatgcttta tacttagatt gaatatgtct
     8401 ataatatctc tagaattaag cacccaatga ttacacgcag taataagcac cttatctgat
     8461 tgattggtac ctatatatat tggtttatta atgtttatta tctctatttt ttcttcggag
     8521 taactatttg aacaaaaaaa gaaattaatt aacactatag agatcagcgc cttcaccttc
     8581 tgttttataa ctaattttat catgttcata aacatcccta tcttctaact cataaatatt
     8641 ttttattata tctacaagtt tctcaattga ttttttttga gagtctgtag cagactccca
     8701 ttttttattt tttttatcat ataatgcaat aacctcaatg ccaacactgt ccatattata
     8761 tggatatcta taaggatatt gtttaatttt ttcatgatca tgtatcttct tagcattcca
     8821 tccgaatgcc tctgttttct ttttttcttc agacgaccat gttttttcct ccttacatcg
     8881 agactttatc ttccccacat ggctggtata tacaaatagg cttgcagcct ggtatatatt
     8941 accatctttt tcaacaataa aatgagttcc aatgccttta gaaaaactag caagtgtacc
     9001 atttattgtt gaagaatctg ttctatgcaa tattattgca ttaattttag gcatgcgacc
     9061 ttcttcaagt tcagggactg tgtgttttat aattccagca tttcttatat acccctcatg
     9121 atcaataaat acaactgata cagtacattt ttcgtcttct tttttctctt cactaagta
//
LOCUS       DAZGTJ010000147         9146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000147 DAZGTJ010000000
VERSION     DAZGTJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.147"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            228..518
                     /locus_tag="ACHK8E_004722"
     CDS             228..518
                     /locus_tag="ACHK8E_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0346887.1"
                     /translation="MSTTASIRKAPRENQINIRATDEERAVIDYAASLVNKNRTDFII
                     ELAYQEAKNIILDQRLFVLDDERYHSFIRQLEAPVQNAEGRERLMAVKPEWK"
     gene            509..991
                     /locus_tag="ACHK8E_004723"
     CDS             509..991
                     /locus_tag="ACHK8E_004723"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0346888.1"
                     /translation="MEINVTAPVLLTDEHILLPFDCGNEVLSNWLRSRAMKNQMFNAS
                     RTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGY
                     GFGKWLLSDAIHRVASLAEQVGIKAVMVHAIDDDARGFYERFGFVQSVVASDTLFYKI
                     "
     gene            1275..1532
                     /locus_tag="ACHK8E_004724"
     CDS             1275..1532
                     /locus_tag="ACHK8E_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0346889.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            complement(1568..1687)
                     /locus_tag="ACHK8E_004725"
     CDS             complement(1568..1687)
                     /locus_tag="ACHK8E_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFP0346890.1"
                     /translation="MSKIIGVFAFLAPVHPHQNQFPATLWRGQPQNSLKRSAI"
     gene            1766..1840
                     /gene="tap"
                     /locus_tag="ACHK8E_004726"
     CDS             1766..1840
                     /gene="tap"
                     /locus_tag="ACHK8E_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0346891.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCG"
     gene            1833..2690
                     /gene="repA"
                     /locus_tag="ACHK8E_004727"
     CDS             1833..2690
                     /gene="repA"
                     /locus_tag="ACHK8E_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0346892.1"
                     /translation="MADLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDI
                     SEEAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            3590..3820
                     /locus_tag="ACHK8E_004728"
     CDS             3590..3820
                     /locus_tag="ACHK8E_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFP0346893.1"
                     /translation="MHKRMTITLDEAVYEGLYRTVGKRRMSQFIEDLLRPHVVDSSLD
                     DGYKAMAEDTERETLAMEWCNALAGDMADAAR"
     gene            3807..4133
                     /locus_tag="ACHK8E_004729"
     CDS             3807..4133
                     /locus_tag="ACHK8E_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP0346894.1"
                     /translation="MRRGEAWWVEFEPAIGSEIRKTRPAVIVSNDAANRNLARVVVVP
                     LTSNTERLYPGEAVVTVGGRRSKAMADQIMAADKSRLKAHIDTLSKADMLLVEDAIKI
                     HLALPR"
     gene            4915..5169
                     /locus_tag="ACHK8E_004730"
     CDS             4915..5169
                     /locus_tag="ACHK8E_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFP0346895.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            5169..5585
                     /locus_tag="ACHK8E_004731"
     CDS             5169..5585
                     /locus_tag="ACHK8E_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0346896.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCARLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            5972..6160
                     /locus_tag="ACHK8E_004732"
                     /pseudo
     CDS             5972..6160
                     /locus_tag="ACHK8E_004732"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(6231..7112)
                     /locus_tag="ACHK8E_004733"
     CDS             complement(6231..7112)
                     /locus_tag="ACHK8E_004733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP0346897.1"
                     /translation="MDELTLKVVGLFFISCIGFVYGSLTKAPAKSIADILIKIISPVV
                     VVVSIVRSPADFLYLKFSVGAFLFCSLLCLIAYFLSSPLFKGKERNLFAFTAGTGNTG
                     YFALPIVLSLFNENQVAIAIFIIIGVNLYEFSVGYYVMSMNGGIREAIQKTLKLPVIH
                     AAILGIVIKYSGVHLNNDILYVLDNFKGAYSVMGMIIIGMVLSVNAKSIPDFKFLLVA
                     LSWKHLLVPVASCVFIFIPHEQFLVVFLMALTPLAGNTVVFSSSLDLHPEKAASAVLL
                     SSLLSIVLIPVSMKIIS"
     gene            complement(7119..7985)
                     /locus_tag="ACHK8E_004734"
     CDS             complement(7119..7985)
                     /locus_tag="ACHK8E_004734"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFP0346898.1"
                     /translation="MNKWYDEINNIADRIDKEAVAHAIEITREYISNINDISYELEKG
                     CGYYIIKNVPVDDYMPEPPSNGLRPYNKRYVSELSLAGITGALGYNVFSYAQEKKGAF
                     FHDITPVSGKEETRSSNGTVDFSFHTDAAYLKRDIRPEILSLLCLNNDSKTATQIISL
                     DRLLDNIPESTINVLKTNEFVISSPDSFDIREENRTSILYEHNGELEIQLSIEVTRAL
                     NTDAEKALKLISEAANAASSSLMWDKGDFLIFNNRKTLHGRREIKGKRWLQRCYGSKN
                     IPLYKTINLEGL"
     gene            complement(8456..8654)
                     /locus_tag="ACHK8E_004735"
                     /pseudo
     CDS             complement(8456..8654)
                     /locus_tag="ACHK8E_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8644..8754)
                     /locus_tag="ACHK8E_004736"
                     /pseudo
     CDS             complement(8644..8754)
                     /locus_tag="ACHK8E_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089474.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
     gene            complement(8950..>9146)
                     /locus_tag="ACHK8E_004737"
     CDS             complement(8950..>9146)
                     /locus_tag="ACHK8E_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346899.1"
                     /translation="FLRELAESLPRILPEGSTDKSALLQRLANEELARAEYGEQVRGV
                     VRRKRRNFREAQILCSATCT"
BASE COUNT         2604 a         2049 c         1982 g         2511 t
ORIGIN      
        1 acaggttaag gcatttcctg tttttcagcc atttcgcaac aaaaatcaca ttaaacaaca
       61 gtaatcagac atgatttgtg cgccgacacg tatcatcgtt acaactttca ggtatctatt
      121 attattttta ttagggagca ctttccccgt gagtgatttt gtatagcgaa cagttactat
      181 ttgcggtaat atttattgta tagcagtttg ttaccaatgg gggaattatg tccactacag
      241 caagcatccg taaagcaccg cgagaaaatc agattaacat ccgggcaacg gatgaggaac
      301 gagccgttat tgactatgct gcgagtctgg tcaataagaa ccgtaccgat ttcatcattg
      361 aactggctta ccaggaagct aaaaacatca tcctggacca acgtttattt gtgctggatg
      421 atgaacgtta tcattcattt attaggcaac tggaagctcc tgtgcagaat gcagaagggc
      481 gtgaacgtct gatggcggta aagcctgaat ggaaataaat gttaccgcgc cggtattgct
      541 gacagatgag catatacttc tgccatttga ctgtgggaat gaggtgttaa gtaactggtt
      601 gcgtagtcgg gctatgaaaa accagatgtt caatgcttct cgcacgttcg ttatttgcct
      661 ggagggtact ttacgtgttg tagggtatta ctcattagct actggttctg tcacacatgc
      721 cgagcttggg cgcagtttgc ggcataatat gcccaatcct gtacctgttg ttcttttagg
      781 gcgcctggct gtcgatgttt gtacccaggg atatggtttc ggtaaatggc tgttgagtga
      841 tgccattcat cgggttgcca gtctggctga acaagttggc attaaggctg ttatggttca
      901 tgcaattgat gatgacgcca ggggttttta tgagcgtttt ggctttgttc agtcagttgt
      961 ggcgtctgat accttgtttt ataaaatttg atgttacgca attttattag gcgtagcttt
     1021 ataatgatgt aaatcgatag tccctcggac tggcgttcac atttcagaaa tcatcggtcc
     1081 cacccggggc cggcacacag gttaaggcat ttcctgtttt cagctatttc gcaacaaaat
     1141 tcacattaaa caacagtaat cacagttgat ttgtgcgcca acacagatct tcgtcacaat
     1201 tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc ctgtctgaga
     1261 agagaggagg ctagatgtcg cagacagtaa atgcagagac ttcctcatcg ggaaaaaaac
     1321 gaccttacag aaggggtaat cctgttcctg cgagagaacg acaaaaagcg tctcttgcaa
     1381 gaaggagtgc cactcataaa gcgtttcatg ccgtcattca gcttcgactg aaagaaaagt
     1441 taagtgagct tgctgatgaa gacgggatca ctcaggcaca gatgcttgag tggctgatag
     1501 agtcagaggt taagcgcaga aaatctttgt gaatatttgc gtttcttgct gtatcagtga
     1561 tgagatgtta gattgctgat cgttttaagg aattttgtgg ctggccacgc cataaggtgg
     1621 cagggaactg gttctgatgt ggatgtacag gagccagaaa agcaaaaacc ccgataatct
     1681 tcgacaagtt tggcgactaa gaagattacc ggggcccact taaaccgtat agccaacaat
     1741 tcagctatgc ggggagtata gttatatgcc cggaaaagtt caagacttct ttctgtgctc
     1801 actccttctg cgcattgtaa gtgcgggatg gtgtggctga tcttcaacaa acgtattacc
     1861 gccaggtaaa gaacccgaat ccggtattca caccccgtga aggtgccggg acgctgaagt
     1921 tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat tttgccgcgc
     1981 atgttgcgta tgcacgttcg cgtggcctgc gccggcgtat gccgccggtg ctgcgccgca
     2041 gggcgattga tgcgttgctg caggggctgt gtttccacta tgacccgctg gccaaccgcg
     2101 tccagtgttc catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg
     2161 gaaaactctc catcacccgg gccacccggg ccctgacgtt ccttgcagag ctgggactga
     2221 tcacctacca gacggaatat gacccgctta ttggctgtta tattccgact gacatcacgt
     2281 tcacaccggc tctgtttgcc gccctcgata tttccgaaga ggcggtggcc tccgcacggc
     2341 gcagtcgcgt ggaatgggaa aacaggcagc gcaaaaagca ggggctggat gcgttgggta
     2401 tggatgaact gatagcgaag gcctggcgtt ttgtgcgcga gcgtttccgc agttatcagg
     2461 cagagcttaa gtcccacggc atgaaacgtg cccgtgcgcg ccgcgatgca ggcagaaccc
     2521 gccaggacat tgtcaccctg gtgaaacggc agctgacgcg tgaaatcgcg gaagggcgtt
     2581 tccggggaag cctggaggcc gtgaagcgcg aaatcgaccg tcgcgtgaag gaacgcatga
     2641 taatgtcgcg gaacaacaac tacacacggc tggccactgc ctccccctga gttttacctc
     2701 atctgaataa tccggcctgt gccggaggct tccgcacgtc tgaaggccgg cgacgcacaa
     2761 aaaagcagcg cagcacacaa aaaacagcct catcatctgc tttccggctc gccatgctcc
     2821 cactgttttt cacgcaaaac acgtcttact gacggggaat tccgcttatc cacatttaac
     2881 tgcaagggaa ttctccataa ggttacaatc gttcatggca taaagcgcca gccgtcagcg
     2941 ttacagggtg caatgtttct tttaaacccc agtactgtat ttcctttaaa ctacttaatt
     3001 acttccattt aaaaagaaaa cctgttcact gcctgtcctg tggacagaca gatatgcacc
     3061 tcccaccgca agcggcgggc cccgaccgga gccactttag ttacaacact caaatacaag
     3121 caccggaaaa aacccggccc agcgcagaac tgaagccaca aagcccctcc ctcataactg
     3181 aaaagcgccg ccgcccccgc ccgaagggcg ggaacagagt cgcttttaat catgaatgtt
     3241 gtaactacat tatcatcgct gtcagtcttc tggctggaag ccctcagaac acgctcgtaa
     3301 gcggccctga cggcccgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg
     3361 gaccactccg accgcgcaca gaaactctgt catggctgaa agcgggtatg gcttagcagg
     3421 atgaggatgg gtaaggtgaa atctatcaat cagtaccggc ttacgccggg cttcggcggt
     3481 tttactcctg tgccatatgc aacaactgag taccgccttc catgccgctg acgcggcatc
     3541 aaaatatgct aatatacgtt atacgtatta cttgtcagga atgtgaatta tgcataaacg
     3601 tatgacgatt acacttgatg aagctgttta tgagggactt taccggaccg tgggaaaacg
     3661 ccggatgagt cagttcattg aagatttgtt gcgtccacat gtggtggatt catcgcttga
     3721 tgatggttat aaggctatgg ctgaagatac agagcgggaa acgcttgcaa tggaatggtg
     3781 taatgcactg gctggagata tggctgatgc ggcgcggtga agcatggtgg gttgaatttg
     3841 agcctgctat tggcagtgaa atacgcaaaa ctcgtccggc tgtgattgtg agtaatgatg
     3901 cggccaacag gaacttagcc agggtcgttg tggttcctct gaccagcaac actgagcgac
     3961 tgtatcctgg tgaggcggtt gttactgtgg gagggcgacg cagtaaagct atggctgacc
     4021 agattatggc tgcggataag tcccgactca aagcgcatat tgatacgctt tcaaaagccg
     4081 atatgctgct ggtcgaggat gcgattaaaa tccatctggc tttacccagg taggggttct
     4141 gcgcccattt cctccaaaag tctcactctt agcataaaaa ggggtccgct tggaaaacgg
     4201 aaaatatccc acgctaagac tgttttttgt acgattcgat atcgacactg cgtaatggtc
     4261 ggtacttccc tgtacccagt ttaagattat tacgccagac ataatcccca ttaagattga
     4321 tatgctccca gcccaacggc gaaaggtgag agagcagttg gacccgcggg ggcatttgaa
     4381 cagaaactta cggtttcaac tgctgaacaa aggatgaaac cgtaatttct tgaatgtgag
     4441 tttatccggc tctggctgct gcaatatatt tatacggggt tccaggccgg aaacctggat
     4501 ttttccgcga ccagtaacgg cgtgggttgt cacataaacg accgattgag tgactgtcac
     4561 tgaaacaacg atttctgcat cagatttcgt cacataaccc gtcacctatt caacgtcatg
     4621 caaatctgtc aattactggc tttggttacg gaattttcta ggtttccggc ttagaacccc
     4681 cttttcaagg ttttcagccg tgtaacacct gccgcctaaa aaaagccgca tggcctccct
     4741 ggttttgttt ctcacatatc atccctcatt aatgctaatg tatgcataca ttagcattaa
     4801 tatctgatag caacaagagg cgaagactac ccctaatcaa gtacatttct tgcattgtac
     4861 tatttcatgg cctacaatgt agtaacgtac ctatatgcat caaaaggcaa caacatgaag
     4921 attacaaccc tatccagtcg ggaacttaat caggatgtaa ctcgagccaa acgcgccacg
     4981 aattatggtc ccgtatttat tactgatcgg ggtaaaaccg cacatgtact tcttagtatc
     5041 gaagaatacc gaaaattgac gcaacaacga cgaactctca ttgacatcct ggcgatgccc
     5101 gatgctgatg cagttgaatt tgaaccgcct cgtattacgg ttagtttaaa acctgcggag
     5161 tttttatagt gtatattctt gatactaatg ttatctctga gctacggaag gctcgaacag
     5221 gacgtataga taataatgtt gcaaaatggg cattgcatac agatgcggca gatttcttta
     5281 tctctgttat ttccataatg gaacttgaac agggaatatt acaagttgaa cgtcgagata
     5341 tgcgacaagg gattatgcta cgttcctgga tggatcagca tgtgttacca gcgttttctg
     5401 ataagatact tcctgtagat actgcggttg cattacgttg tgctcgttta catgttcctg
     5461 acaggcgcag tgaacgagac gcattgatcg cagcaacggc gctggtacat gggatgacgg
     5521 ttgtaaccag aaatgttggt gattttgaat taaccggagt atcaattatt aatccctggg
     5581 cttgatatcg cttcccttct tctagggctc cgtttgggaa acggaatcgt actataagag
     5641 cgtaaggtgt caggttgaag tcacagactc cgcgtcagga gtgcccagaa aattcgaaat
     5701 agaccctatt ctaacgtgtt tcgcccaaac aacctgacgt cagactgaag tacactccaa
     5761 cctgacagca gtatgagcaa caacaagaat ggtgcagctt tggcttcgat agctgtctct
     5821 tagcgtacaa aaatatccgt ttcgtgagtt gaccccctat acggcgtttt cagaggattt
     5881 gactttaatc agatcaccta aatgcgatac cgaaagatga tgctgagcaa cggggattgc
     5941 cttttgatgc aaaaatactg aaaaagcgtt aaggctttac accagtcgtt acgcatacca
     6001 gaaggcgttg cgaaagatga aagaacgcat agaccttgac ggaagagcag caggagaagt
     6061 cacgccagaa gaaaaggaac aggcaaagat ccatataatg ggaatagatg aaaaacagaa
     6121 gcgaagggca gaagaaaaaa gacgtcaaca aacaaagtag aactgttaaa tatagattat
     6181 ggtaagcctg ttctaaacac aggcttacca ttgacaaatg gtattcactc ctagcttatt
     6241 atcttcatac ttacaggaat taacacgata gataataacg aactaagtaa cacagcagat
     6301 gcagcctttt caggatgtaa atcaagacta ctactaaaaa caactgtatt accagccaat
     6361 ggtgttagcg ccatcaagaa tacaactaaa aattgttcat gtggaataaa aataaaaaca
     6421 catgaagcca ctggtactaa taaatgtttc catgataatg caactaaaag gaatttgaag
     6481 tcaggaattg attttgcatt tacagataga accatcccta tgattatcat tcccatcaca
     6541 gaatacgcgc ctttaaaatt atcaagcaca tatagtatgt cgttatttaa atgaacacct
     6601 gaatatttta ttacaatacc aagaatagct gcatgtataa caggtagttt taatgttttt
     6661 tgtattgcct ctctaatgcc tccattcatg ctcatgacat aatagccaac actaaactca
     6721 tacaagttaa caccaataat aataaatata gcaatggcca cttgattttc attaaataga
     6781 ctcaacacta taggaagtgc aaagtaacct gtatttcctg ttcccgctgt aaacgcaaac
     6841 agatttcttt ccttaccttt aaataaaggt gatgataaaa aataagcaat taaacaaagc
     6901 agtgagcaaa acaaaaatgc cccgacagag aattttagat ataaaaaatc agccggagat
     6961 ctaacaatac taacaacaac aacaacaggc gatattattt ttattaatat atcagcaatg
     7021 ctttttgctg gggcctttgt caaggaccca tacacaaaac caatacacga tataaaaaac
     7081 aaaccaacta cttttaacgt taattcatcc ataaataatt ataatccttc taaatttatt
     7141 gttttatata gcggtatatt ttttgaacca taacaacgtt gcagccatcg tttcccttta
     7201 atttctctcc gtccatgtaa agtttttctg ttattgaaaa ttaaaaaatc gcccttatcc
     7261 cacatcaaac tagatgatgc tgcatttgcc gcttcagaaa ttaatttcaa agccttctct
     7321 gcatcagtat tcaacgctct agtaacctct attgataatt gaatctccaa ttctccgtta
     7381 tgttcgtata atatagatgt tctattttct tctcttatat caaatgaatc aggagaacta
     7441 atcacaaact cattagtttt caaaacattt attgttgatt ctggaatatt atccagcaat
     7501 cgatccaatg aaataatttg cgtcgctgtt ttactatcat tattaagaca taacaatgat
     7561 aaaatttcag gtcttatatc tcttttaaga taagcggcat cagtatggaa cgaaaaatca
     7621 acagtcccat tactcgatct tgtctcctct ttaccgctaa ctggggttat atcatggaaa
     7681 aatgcccctt tcttctcttg agcataagag aatacattat atcctaatgc ccctgtaatc
     7741 cctgcaagag atagttcgct gacatatctt ttattatatg gtcgtaatcc attgctggga
     7801 ggttcaggca tataatcatc aacaggaaca ttttttataa tgtaataacc acaccctttc
     7861 tctaattcat aagaaatatc atttatatta ctaatatact ccctggtaat ctcaatggca
     7921 tgagcaacag cttccttatc aatacgatcg gctatattat taatttcatc ataccattta
     7981 ttcatgttca ctctccttta acttgataac aaacattatt taaaaaatat ttgctatcat
     8041 tttttattct ttttaactca atgtttttat gcacaacaaa aatttctgcg cttaatatag
     8101 ataacagcaa ggcgcttatt aatatacttt taaatatcat taacacacct cacaaaacaa
     8161 caatcccaca ttcttcttac aacacaaaaa acaaaaaatt aacacactgt gattgtttta
     8221 ttgaacttca tcacacttta attattcaca tcacgataac cccataatga atttataact
     8281 tttgaaactt aaaaaaataa taaaactaaa aatataaatt ctcatttttt ataaaaatac
     8341 atgagatcag attggacagc ctttacgttc agtgagttag ctggcttgga tgtaaccact
     8401 cgttcaactc aattctctta gcgtgggaaa atttccgaat tccaagcgga cccctaatac
     8461 ggcgaacccc ttcaccatag cgttcatccg tttcatacag cctggggtaa tctgccaggc
     8521 gatcggttgc agtgatcaga cgctctgtca cgttccttgc atcccaaaga gatgcattct
     8581 ttctcggcca gatattcaaa caacgccgtt atatcctcaa tcgcacctgg tcgccattct
     8641 atttcataac tcattgcgca gttcctgata acgtccctgt agttgctggc gcagttgcgc
     8701 ggaacttatt cccggtcgct tatcggctcg agcagcggca attttcgctc gtaggggtta
     8761 tacgccggaa acgccgaaat ttccgataca gcgaatttgt tgatttttga agcatgtttg
     8821 gcggttgttg acgagtacgt ttatggtgca gtaagtgaga atttatccgt accagctatg
     8881 tgatacgtta cgggcagtga ttctttcgct ctaaacagct atttctttgc tgtccggtta
     8941 acggcggtct tacgtacagg ttgccgagca taaaatttgt gcctcccgga aatttcggcg
     9001 tttccggcgt acaacccctc gtacctgttc gccgtattcc gcccgtgcca gttcttcatt
     9061 agcgaggcgt tgcaatagtg cagacttgtc tgtactgcct tctggcaaaa tacgaggcag
     9121 gctttcggcc agctcgcgca ggaaat
//
LOCUS       DAZGTJ010000148         8949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000148 DAZGTJ010000000
VERSION     DAZGTJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.148"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..531)
                     /locus_tag="ACHK8E_004738"
                     /pseudo
     CDS             complement(1..531)
                     /locus_tag="ACHK8E_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            867..1229
                     /locus_tag="ACHK8E_004739"
                     /pseudo
     CDS             867..1229
                     /locus_tag="ACHK8E_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            1230..1588
                     /locus_tag="ACHK8E_004740"
                     /pseudo
     CDS             1230..1588
                     /locus_tag="ACHK8E_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766597.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            1871..3010
                     /locus_tag="ACHK8E_004741"
     CDS             1871..3010
                     /locus_tag="ACHK8E_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFP0346900.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDHDALHNLMEYFEYRACDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            3130..4398
                     /locus_tag="ACHK8E_004742"
     CDS             3130..4398
                     /locus_tag="ACHK8E_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFP0346901.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEVISHDNHGKQ
                     PGFLAAISAPLVAIVLLTLRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLIFGVFGLAG"
     gene            4406..5551
                     /locus_tag="ACHK8E_004743"
     CDS             4406..5551
                     /locus_tag="ACHK8E_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP0346902.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTRIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            complement(5969..6778)
                     /locus_tag="ACHK8E_004744"
     CDS             complement(5969..6778)
                     /locus_tag="ACHK8E_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0346903.1"
                     /translation="MSGSVMHSQSAAMVPAAYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPLERRNKR
                     TGRTEKARIWEVTDRTARTWIREAVAAAAADGVTFSVPVMPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPEVDAVAMLKNRQV"
     gene            complement(6779..7084)
                     /gene="ccdB"
                     /locus_tag="ACHK8E_004745"
     CDS             complement(6779..7084)
                     /gene="ccdB"
                     /locus_tag="ACHK8E_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP0346904.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGNESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(7086..7304)
                     /gene="ccdA"
                     /locus_tag="ACHK8E_004746"
     CDS             complement(7086..7304)
                     /gene="ccdA"
                     /locus_tag="ACHK8E_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP0346905.1"
                     /translation="MKQRITVTVDSDSYQLLRAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            7901..8158
                     /locus_tag="ACHK8E_004747"
     CDS             7901..8158
                     /locus_tag="ACHK8E_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346906.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADSQRLRNLIWPEDDR"
     gene            8158..8748
                     /locus_tag="ACHK8E_004748"
     CDS             8158..8748
                     /locus_tag="ACHK8E_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346907.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESTAP"
     gene            complement(8768..>8949)
                     /locus_tag="ACHK8E_004749"
     CDS             complement(8768..>8949)
                     /locus_tag="ACHK8E_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP0346908.1"
                     /translation="LWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFH
                     WWRRKVNRLPPWDDV"
BASE COUNT         2180 a         2350 c         2328 g         2091 t
ORIGIN      
        1 tcttccagcc agtcagccgg atcgctatgt gcggcagcag tctgtttcag aatgcgggtg
       61 tcgaaccggg cattaaagat aattactggt tggtctccga tcgcatgacg cagttgccga
      121 gccacatctg tccatgatgg tgcgccgcgg agagcctgct cactgatacc atgcacggcg
      181 gcggcctgcg ccccgatgga gaccgagggc ttcagtctgg tttcaaatac cacctgaccg
      241 gccgcatcag taaggccgat ttccagggct tccgcggtat tatccagtcc tgtcgtttca
      301 gtatccagaa agagcggtgc cagactcagc cagtcatatg cctggtgcca tcctgccact
      361 ggtgctgttt agtctggata aaacggaaag ccgctggcca gccagttgtt gtttactcgt
      421 cagtgggcgt ttctcacgca tcggaacaca gtctgcaatt cggtatacag cgaacctgcc
      481 actataagtg gttttatacc acttaaccgg ctcagcaccg ggagctggct tcgtaccctg
      541 cgtgcatctg atctgtggct ggccgtggca cggctacgga cagattctgc tccgcaacag
      601 tatcggcgtg cctgacctcc tgaaagtccc gtaacgcatt ttctctcctc ccatgccctg
      661 acgctattga gcgcgttaca gaacaattta ggtcatttaa cgagcctcca acggctcgat
      721 cgaaataaat caaatcgtaa tcgtcaacga tatatgagag gagaccccgc tcgccgcaaa
      781 tcgatgaccg aacgtagtga gcgaatcgac gaggaagcgg aagagaacct gtacgaaaat
      841 ggagcaatta cgccccaccg tcaagaaatg agctggctct ccgcggcgcg ccatcatata
      901 cagatgtggc tcggcaactg cgccatgcga tcggagacca accagtaatt atctttaatg
      961 cccggttcga cacccgcatt ctgaaacaga ctgctgccgc acatagcgat ccggctgact
     1021 ggctggaaga aatgacggta tattgtgcga tggagctggc tgcaggatat tatggggcca
     1081 ccaaccgcta tggcacgatt tcactggcca gtgctgccag ccaggccgga ctgacatggg
     1141 aagggctggc acactcagcg atcgccgaca cacggatgac gtcaggggtg gtaaacgcta
     1201 ttactgcata tcatcaggaa ttgctgcagc ttatgtactg cgataaccct gaactcaact
     1261 atgcaaacct gaaatccctg caaaaacagc tggatgaaat gtatacctgc atccgtaata
     1321 atcatatgat gctcccaaac tacggaaaaa tgcgccggta tggtgaaccg gtttcaacag
     1381 cgtttgtaga gtcgacaatc aacgaagtga tcgccagaca atgacccaaa agcaacagat
     1441 gcagtgggat agggaaggta cgcattattt gttacagacc agaaccgctg tcctgaataa
     1501 ttaactgcag gacaaattcg ccagttggta cccgggttgc acgatagacg ctcagagtgg
     1561 aggaaagtta tccgcgatgg cagcataact acttccccag gttttttcat gctctccctt
     1621 accaatatcc tatgacgatc acatcgaaaa aaaacacgga acaggcgaac agaaatataa
     1681 ttaattattt gagtttaaag gaaaaaagag gatacaagct ttttgtggca tttttatttg
     1741 tataaaccga caaaaaagag gccgtgcttc ccgctttctt tgtctgttta cacggaacat
     1801 ttcaccactg gatacgctaa tagatcggat atgagcacat attgcacccg cctttcatgc
     1861 agagcataaa atgaacaaga ttcacgctaa ttattacctg caagattcta ccgcacgcca
     1921 gatagttcaa cgcacaatgg ggattattcc gtactcggta aatgtgatga acgagcatgg
     1981 gatgatcatt gcttccgggg aagcctcacg tttacatcaa cgccatgaag gtgcggtact
     2041 cgcattgacc gaaaaccggg tcgtcgaaat tgattccgcg accgccaata agcttaaagg
     2101 cgttaagccc ggaatcaatt taccgattaa ttttcgcgac cagctgattg gtgtactggg
     2161 cattaccggc gagcctggtg aagtgaaggc atatgcacag ttggtgaaga tggcttcgga
     2221 attaatcatt gaacaaatgg ctctcctcga gcaaaaacag tgggataagc gttaccgcga
     2281 agagctgatt aatcagctga ttttacgcga tccgccaccg gactcactga atgcggtggt
     2341 ctcatggttg ggaattaatc tccagcttcc gcgggtggtg gtgattatgg aactgcaaca
     2401 acctgatcac gacgcactgc ataatctgat ggagtatttt gaatatcgcg cctgcgacca
     2461 tttggtcacg ttttccgact ttaatgatct gataatctta aaacctatct cgctaaaaaa
     2521 cggcgtctgg gataaatcgt tggaattaag cgatctgcag agacttaaac attacgccca
     2581 gtcctgtggt ttttcccgac tgattgtcgg cggctatttc tctggagaaa acggcctgca
     2641 tcgctctttt cagaccgcga aatccaccca gtccatggcg aaaagactga agttgaagca
     2701 tcagttcatt tttcatgacg actatgcgct ggcatcatta ttagatgggc tttctggtac
     2761 ctggcaagcg gaagaattat ccaaaatctg gcgcaatctg gttgccaagg acaccaaagg
     2821 tatgctgcaa aaaacgctgc agaattattt tgatcataat tgtgatcttt cgcaaacagc
     2881 gactggcttg catattcatg tcaatacatt acgctatcga ctgcagagag tggaagatat
     2941 tacaggaata caaatcaata acttaaagca gttattctgg ctgtatatcg gcatggagtt
     3001 acaacgctga actgtatttc cctccaaatt tttcgccgtc tgacgtcgta tttttagccg
     3061 atacacacaa gtctaacgcg gctgctttct ccatactgcc ccctgttatt ttcatggaga
     3121 aagcagaaga tggtttcaat aacagcaact ggcgccatca tcgcactcgt ggtggcgatc
     3181 gttttaattt tacgcaaagt ctcgccggct tacggcatga ttgtcggtgc gctggtaggc
     3241 ggtctggtcg gcggcgccga tctggtgcaa accgtgacgc taatggtcac tggcgcgcaa
     3301 ggcattacca atgccgtgct gcgcattctc gcagcaggtg tactggccgg cgtgttgata
     3361 gaatccggcg cggcaaacac catcgctgaa actgtggtga aaaaagtggg ggaaagccgg
     3421 gcgcttttag ccttggctat tgcgacgatg attctgaccg ccgtcggagt atttatcgac
     3481 gtggcggtta tcactgttgc gccgattgcc ctgtctattg cccgtcgtgc cggtttgtcg
     3541 aaactcgcca ttctgctggc gatgattggc ggcggtaaag cgggtaatgt tatgtcgcct
     3601 aaccccaatg ctatcgccgt ttcggacacc tttcatgttc ccctcacctc agtgatgatg
     3661 accgggataa tccccggtct gttcggccta atcgtggctt atttcctggc aaaatgtctc
     3721 agccagaaag gcctgatggt gatgcccgac gaagtgattt cacacgacaa ccacggcaag
     3781 cagccgggat ttcttgccgc tatcagcgcg ccgctcgtcg ccattgtgct gctgacgcta
     3841 cgacctgttg cgggtatcgt catcgatccg ctgattgccc tgcccgtcgg tgggttagcc
     3901 ggtgcgatcc ttatgggcag gtttaagcaa agtaacgcct ttatgatgtc cgggcttacg
     3961 cgcatggcgc cggtagccat catgctgctc ggtaccggta cgctcgccgg gattatcgcc
     4021 aactcggaac tgaaagatgt gctcattcat ggactgacct cttccggtct gccttcttat
     4081 cttctggccc ctgtttccgg cgcgatcatg tccatggcaa cagcctccac cacggcggga
     4141 accgccgttg ccgccggggt attcagccag acgctgctgg ggcttggcgt atcagccctt
     4201 gccggttccg ccatgattca tgctggcgca accgtacttg atcacctgcc tcacggcagc
     4261 ttcttccacg ccacgggcgg cagtgtcggc atgctgatcc atgaacggtt aaaactgctg
     4321 ccttatgaaa cccttgtcgg ttttgtggtc gccctgattt ccacactgat atttggcgtc
     4381 ttcggacttg ccgggtaaag acacgatgaa aatagtcatt gcaccagatt catttaaaga
     4441 aagtttatcg gccatgcagg tggccaccgc tattgagcag ggatttcgtg aaatttatcc
     4501 cgaggccgag tatgtcaaat tgccgatggc tgacggcggt gagggtaccg tcgaatcaat
     4561 ggttgaagca accgaaggcc attatctgtt ccagaatgtc accggaccgc tggggacgcc
     4621 agtacaggcg cgctggggta tgctgggaga tgggaaaacg gcgatcgttg aaatggcctc
     4681 cgcatccgga ctacatcatg ttcctccaga atgccgtaac cctatgctta ccaccagtta
     4741 tggcacaggc gaactgatcc ttgccgcact ggaccacggc gttgagcgaa ttattctcgg
     4801 gattggcggt agcgccacca atgacggcgg cgcaggtatg ctacaggcac ttggtgtggc
     4861 gatgcttgat acaaacggga aatcgcttcc tttcggcggc ggcgcgctaa gcaacctagc
     4921 gctcatcgat gcctcaggct gcgacgctcg actgaaaaac gtttctgtca ccgtcgcctg
     4981 cgacgttaac aatccactct gtggcgcaac aggagcgtca gccatcttcg gcccgcaaaa
     5041 aggagcaaca ccggaaatgg ttgcctcctt agaagctgcg ctgaccaact tcggtatacg
     5101 acttcaggac gcaaccggac gaaacgtgat gacgacgcct ggcgcaggtg ccgctggcgg
     5161 catgggcgcg gcgctgttag ggattctgaa tgcggaatta aagccgggag tggagattgt
     5221 tattgacgcg ctgcatctcg atagcctagt cgcggatgcc agcctggtaa tcaccggtga
     5281 aggtcgtctt gatagccaga gcatcagtgg taaaacaccg attggtgtcg cacgctgcgc
     5341 taagcggtat cagaaaccag tgatcgcact ggcgggcggc ttaacgaccg atcaccagat
     5401 agtgcatgag cacgggctgg acgcggtgtt ctccgtgctg acgcggatcg ccactctgcc
     5461 ggaagcgcta caggatgcag aatacaacct ccgcgtttcg gcacgcaata ttgccagcgt
     5521 ctggaaatta tcacagcagc agatgcgcta attttcagtc aggccggcaa gcagttgctg
     5581 gcctggatat tctttctcac caggtccatg ctaaaacagc ctgcttcagt catcccacgc
     5641 aaggcactat catggcaaaa aaaggaggag agtagtgacc cccaaaagac ggttaagcgg
     5701 ttaaattgaa tggtcaataa gggggctgtt tgggaaacgg aaatttttgt actgtaaggg
     5761 cattatttaa ccaatttaag atcctgacgt attgatttaa cgtcagtggc tgtcgcctgt
     5821 attgctatat cgcatttagg ttatccgatt aaggccaaat tccctgaaaa cgccgtatag
     5881 cacgggagta caccctattg cgacaggcag gtctattcaa aaaacaggct tgccgtacaa
     5941 taattctcta tatccaaact gcccccaatt atacctgcct gtttttcagc atggctaccg
     6001 catccacttc aggcatcgca aactgcaccc ggtgccgtgc agccacatcc agtgcaaaca
     6061 ccttcgtgta gacttcagtt gaactgatgg acttatgtcc catcaggctc tgcaggacct
     6121 tcagtggtat accggcatac agcatgtgca tcgcatagga atggcggaat gtatgtggca
     6181 tgaccgggac agagaacgtc acaccatcag cggcagcggc ggcaaccgct tccctaatcc
     6241 aggtcctggc cgttctgtcc gtcacttccc agatccgcgc tttctctgtc ctgcctgttc
     6301 gtttattacg ccgttccaga ggaattttca gtgttgccac catcgtctgc agctggctga
     6361 cgtaccaggt atcggagagc ggaaccagcc ggtgagtctg ctggccggca ggcattcttc
     6421 ctgccgtcct ggcagctttt tccgtccgtt gtttcagggt cgcaagctgc acaaacggat
     6481 acggaggcgc aagcgaaaaa tccccgcgcg tcagtgccag tgcttcatta atgcgcgcac
     6541 cggtgttcca cagtgtggcc agcagcatct tgcggcgcag atccgggacg taatggagca
     6601 gggcactcac ttcgggggcc agcagatatt tcggcagttc atcatgaacc atcgacatct
     6661 ggcggagtgc cagagctgcc ggataatcaa tggcaacagg aagttgtgca gactgtccgg
     6721 cagaatacgc tgccggtacc atggctgcag actggctgtg cataacggag cctgacattt
     6781 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcccgg tggctgagat
     6841 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
     6901 gccagctttc attcccgaca tgcaccaccg ggtaaagctc acgggagacc ttatctgaca
     6961 acagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatgtcac
     7021 tctggacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
     7081 gcatttcacc agtctctgtt ctcatcggca aaagagccgt tcatttcaat aaacctggcg
     7141 acctctgcca tcccttcctg attttcagcc tgccagcgtt cagcgcgcag tcgccgggct
     7201 tcgttctgca tagtcgtact gaccagaccg gagatattca cgtcatacgc cctgagcagc
     7261 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcaca cctctttctg
     7321 acatacttct ggtatgcata taagtatata ttctcatgca gcaaaaatca gcgcgttaat
     7381 acacatactg ttatctggct tttagtaagc cttatgtatt ttaccgtgcg ttatgttaac
     7441 cagtaaaaat taaaacctga cttataaaaa caaagcgtaa ttaccgcatt cccgtttcgt
     7501 atggatccga ctttatttga ataaacgact ttccggaagt gtaccacgct gaggaataaa
     7561 tcatattgct gttttccgcc cctgcgcgac gcatcacagg tcagggagat gacacaacat
     7621 atagtgtcat cagcagctga atccggcacc acatgttgtg cttacaggat gtggcgttgt
     7681 gtggagggag aggaagatcc tgaacagccg gaaaccggat ctgcgcggcg gggaacgggc
     7741 atgcaatatg ataatcaagc aattcacata aaatcatatc agatatgatt ttcatcatta
     7801 aaaataaaac aatatctgat atcatcagaa atcaggagta cacaaatcat acaggatatg
     7861 atttttacct gtcagaacac tatcagatgg agcgtgcagg atggatcagg aaatgacgtt
     7921 ttcactcagc tatgaacaac tgacccggtt tgcggaacga cgcatccggg aatgcaatct
     7981 ggacagccag ggcgcgattt atctctgcga gtcagccaaa gccggagccg tgctgatctt
     8041 ctggcatgaa ctggccataa atggctatgc ctcaatgaat gccataaaga gacaggagct
     8101 cattgacgcc gactctcagc gcctcagaaa cctcatctgg ccggaggatg acaggtaatg
     8161 acccagagcc gccgaccgtc gccactgcag cggagagtgc tgattgtgct ggctgccctt
     8221 gatgaaaaac ggccaggccc cgtgctgacg cgggacatag aacgggtgct ggagcgaagc
     8281 ggggaagccc cggtctacgg gccaaacctg cgcgcctcct gccgtcgtct ggaggatgcc
     8341 ggctggctgc gtaccctgcg cgctccgaac ctgcagctgg cagtggagct gacggacgca
     8401 ggccgggccg tggcacagcc tctgctgctg gcagaacagg accgcctccg ggcagaacag
     8461 cgtgcggcag aagtcgtcgt actgccgctg atcccggcag caggtctgcc cgcagacggc
     8521 acttctgcca ctgatctggc ggtggagctg aacggcataa cgtatcaggc ctgccgtggg
     8581 gattttgtgg tacgtctcga cggcagcacc tgtctgcagt tatggaataa agaaggcagg
     8641 gtggtacgcc gggagggcga tccgctggaa gtggcgcagt ggctgcaggc ctgtcacgat
     8701 gcaggaatgg aggttcgcgt acagataaat gaaagtaccg ctccgtaaga acacgcctgt
     8761 gcacggttta cacatcatcc catggtggca gccggttaac tttcctgcgc caccagtgga
     8821 aagaggccat gagcagtccg aacaaaaaac cacccgtgat acttataata atggcctcac
     8881 ctgccaccat tcctgacggc ccccagtaga taaaccacat tgcacatccc caggaaatgc
     8941 cccataagc
//
LOCUS       DAZGTJ010000149         8942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000149 DAZGTJ010000000
VERSION     DAZGTJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.149"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            222..725
                     /gene="ftnB"
                     /locus_tag="ACHK8E_004750"
     CDS             222..725
                     /gene="ftnB"
                     /locus_tag="ACHK8E_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP0346909.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(803..1054)
                     /gene="yecJ"
                     /locus_tag="ACHK8E_004751"
     CDS             complement(803..1054)
                     /gene="yecJ"
                     /locus_tag="ACHK8E_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP0346910.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1169..1255)
                     /gene="azuC"
                     /locus_tag="ACHK8E_004752"
     CDS             complement(1169..1255)
                     /gene="azuC"
                     /locus_tag="ACHK8E_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP0346911.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1518..1841
                     /gene="yecR"
                     /locus_tag="ACHK8E_004753"
     CDS             1518..1841
                     /gene="yecR"
                     /locus_tag="ACHK8E_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP0346912.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2013..2510
                     /gene="ftnA"
                     /locus_tag="ACHK8E_004754"
     CDS             2013..2510
                     /gene="ftnA"
                     /locus_tag="ACHK8E_004754"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP0346913.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2548..2787)
                     /gene="yecH"
                     /locus_tag="ACHK8E_004755"
     CDS             complement(2548..2787)
                     /gene="yecH"
                     /locus_tag="ACHK8E_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP0346914.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2977..4188
                     /gene="tyrP"
                     /locus_tag="ACHK8E_004756"
     CDS             2977..4188
                     /gene="tyrP"
                     /locus_tag="ACHK8E_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP0346915.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4239..4904)
                     /gene="yecA"
                     /locus_tag="ACHK8E_004757"
     CDS             complement(4239..4904)
                     /gene="yecA"
                     /locus_tag="ACHK8E_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP0346916.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5100..5186)
                     /locus_tag="ACHK8E_004758"
     tRNA            complement(5100..5186)
                     /locus_tag="ACHK8E_004758"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5150..5152),aa:Leu,seq:taa)
     gene            complement(5199..5272)
                     /locus_tag="ACHK8E_004759"
     tRNA            complement(5199..5272)
                     /locus_tag="ACHK8E_004759"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5238..5240),aa:Cys,seq:gca)
     gene            complement(5327..5402)
                     /locus_tag="ACHK8E_004760"
     tRNA            complement(5327..5402)
                     /locus_tag="ACHK8E_004760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5367..5369),aa:Gly,seq:gcc)
     gene            complement(5554..6102)
                     /gene="pgsA"
                     /locus_tag="ACHK8E_004761"
     CDS             complement(5554..6102)
                     /gene="pgsA"
                     /locus_tag="ACHK8E_004761"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP0346917.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6159..7991)
                     /gene="uvrC"
                     /locus_tag="ACHK8E_004762"
     CDS             complement(6159..7991)
                     /gene="uvrC"
                     /locus_tag="ACHK8E_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP0346918.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7988..8644)
                     /gene="uvrY"
                     /locus_tag="ACHK8E_004763"
     CDS             complement(7988..8644)
                     /gene="uvrY"
                     /locus_tag="ACHK8E_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP0346919.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2210 a         2256 c         2075 g         2401 t
ORIGIN      
        1 cagttacatt gatttcatca atgaaatgta aaaatatata aacttgatga tttaagcatt
       61 ttcttatacc cgttcagacg ttattcttat ttcagatcat cgtcagaatt gactccacga
      121 tcacatttcg gaccggcaga aaggaattat tctgcaaaca gtaattatgg tgttttgatt
      181 tatcttgcac ctctccactt ctggatataa ggatattagg tatggcaacc gctggaatgc
      241 ttctcaaact caactctcaa atgaaccgcg agttttacgc atccaatctc taccttcacc
      301 tgagtaactg gtgttctgaa cagagtctga acggcaccgc cactttcctt cgcgcccagg
      361 cacagagtaa tgtgacccaa atgatgcgca tgtttaactt tatgaagagt gtcggcgcta
      421 cccccatcgt taaagccatt gatgttcccg gtgaaaaact gaactctctg gaagaactgt
      481 tccaaaaaac gatggaagaa tacgagcaac gttctagtac gttggcacag ttagccgatg
      541 aagcgaaaga actgaatgat gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac
      601 agcagcatga tggcctgttg ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg
      661 cgggtatgtg ccctgtgcag accgaccaac atgttctgaa tgtcgtgtcg catcagctac
      721 attgatcatc atcggcgcta atgcattgcg ccgatgaagt ttttgagaaa ccgctgcctc
      781 atttttttga agcagcggtt ttttaatggg attcgccctg tggggtaaac ttgagttcaa
      841 tcaacgcgat ggctttttgg attgcccgca tggtgaccgg gtctgcggcg gcgggatggt
      901 tagtaaagtc gatattcttt agctggctgg acattttttc gcgaacttca acgggtgcga
      961 tcacatcgag aacatccaga atttgtttga taaccaactg gcaagcaacc acatcagaaa
     1021 ccagttcctg atcggcattc agcggctggg acatcgtaaa ctcctgatag cattttgaaa
     1081 gccgttatag tagcggcttc acatcttcag cgatagtcac atccaccgtc atcaggacac
     1141 aaaaaaacct gccggagcag gttttttgtt atcggaacat attgcctggc ggtacgtctt
     1201 tgaacgtctt gcaatagtta ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg
     1261 cactccgacc atttgttata caggtgttat tgtctttgcg cttataatgt gacaaccatc
     1321 acaaaaatca atctttatgt gatgcaaatc acataaatac ccctttaatg ttataaaaat
     1381 gataatcaaa aagcagctac cccatttctg acacctacag atggcaagaa atagcgcctg
     1441 ccaggcgtct tttccggcca ttgtcgcagc gctgtagcgc gtaaaatagt gctttctttt
     1501 actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata acgctggcgg
     1561 ggtgtacggt tactcgccag gcccatgtga gcgaagttga cgcggcaaca ggtattgttc
     1621 ggttggttta tgatcaggct tttttgcagc atgcccgtac cgatcgttat gtcagtcgcg
     1681 ggatcgccga tcgcgcatgc cagcaggcag gctataccca cgctatcccc tttggtcagc
     1741 cagtaggcaa ctgtagcctc tttgcgggtt ccttatgttt gaatacggaa ttcactttgt
     1801 catatcagtg ccatcattcc gccttccctg tctttctcta aacgttttaa tcagcgaggg
     1861 ggatcttcgc tgattaaata aatagcggta atgcgtttta ttcccattcg tatttttaat
     1921 aaatgatgtt tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac
     1981 agcaacaaat ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac
     2041 ttaatgagca gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct
     2101 ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag
     2161 agatgacgca tatgcagcgt ctgtttgatt acctgactga taccggcaac ttaccgcgta
     2221 ttaataccgt tgaatctccg tttgctgaat attcctcact tgatgaatta ttccaggaaa
     2281 cctataaaca cgaacaatta atcacgcaga aaattaacga actggctcat gctgcaatga
     2341 ccaatcagga ctacccaaca tttaatttcc tgcaatggta tgtttctgag cagcatgaag
     2401 aagagaaact gttcaaatcg attattgata aattaagcct ggcaggcaaa agtggcgaag
     2461 gtctgtattt tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg
     2521 cggcaggttt tctgccgccg tttcaaatca gtgacggcaa atcttactct gatcggttga
     2581 aaaaccttct tctgaaggta taaatttgcc ttttgctgcc agaaacgcca ccaactctcc
     2641 cgcagtcatc ccttctgccg agcaggtgtg aaaacgtgcc tgttcaccaa aacgcgcttt
     2701 aattgctgct tccagactgg catgcgtata ttgctcgcct gattcaatca tcatatttaa
     2761 cacttcgtga ccgtgaatag agtccatcgt tcctcctcaa aaaaagccta gcgtagcgat
     2821 tgccgcttat gaagactttg cgccagcgca gaactgaacg cttttattgt acatttatat
     2881 ttacatcata tgtaacgccg gtttgacgaa tcagccgtta tgccttaacc tgcgccgcag
     2941 atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac tctgggaagt
     3001 gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg
     3061 gcaggtgttg gttttagcgt cacgttaatc ttgttaattg ggctttgggc gttgatgtgc
     3121 tacacggcgc tattactgct ggaggtgtac cagcatgtcc cggcagatac cggtctgggc
     3181 acgctggcaa aacgctatct gggacgctac ggtcagtggc tgacgggctt cagtatgatg
     3241 ttcttaatgt atgctctgac tgcggcatac atcagcggtg ccggtgaatt gttggcctcc
     3301 agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact
     3361 tttgttgccg gtggcgtggt ttgtgtcgga acttcgctgg tcgatttatt taaccgtttt
     3421 ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt
     3481 cacaaagtga atcttttaac cctgccgttg caacagggac tggcactgtc tgcaatcccg
     3541 gtgattttta cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat
     3601 ggcaacattc gtaagctacg ctgggtgttt ataatcggta gtgcgatccc cctggtggca
     3661 tatattttct ggcagctggc gacccttggc agcattgatt caacaacctt tatgggattg
     3721 ctggctaatc atgccggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct
     3781 ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac gtccttcctc
     3841 ggcgtagcgt taggcttatt tgattatctg gcagatttgt ttcagcgttc aaataccgtt
     3901 ggtggacgat tgcaaactgg tgcaattacc tttctgccgc cactggcgtt tgcactgttt
     3961 tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca
     4021 ttgattatcc cttcgctgtt gacctggcaa agcaggaagc acaatcctca ggcgggttac
     4081 cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt
     4141 ggcgtgcaat ttttgattgc ggcaggcttg ttaccagaag tggggtgatt agattcttat
     4201 tgggtgccag aattaacgct gacacccaat ttgacctctt aatgcaggca gcactgctta
     4261 aatttcttac cactaccgca cggacaagga tcgttacgcc ccggtttctc ttctgctttg
     4321 ataggttgct gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg taaatcaagc
     4381 gccgccagtc gaatagcatc tacgctctct tcaaacgctt ctggcgacat cttctctacc
     4441 cgctcgaagt tttcctcagt accgtgcagc gcaatcgcat ccagcgctgg ttttaacgaa
     4501 tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc
     4561 tcaacaatcg tcagctcgct gccatcgact tctcgcaagc cgaataacgg ctcaaactgc
     4621 tccgggaatt cgttcagacg ctctgcggta tcagccatat gttgaaaagc caggttcata
     4681 aagcgcgtca tctctttctc tgacgcccag cgcggcacat agtcagcccc accccacacg
     4741 gcaaccagcc actgtgccga ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa
     4801 ccgtccagct ccgccacatc aagaatggcg tggtcagtgt tgtatttggt cagaatatcg
     4861 tccagccatt ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg
     4921 atctcaactc gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg
     4981 gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac
     5041 cacccgaagg tggtttcacg acactgctta ttgctttgat tttattctta tctttcccat
     5101 ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt taaatccctt
     5161 gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga
     5221 accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg ccaaattctt
     5281 caggcctttc agccagacat ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg
     5341 agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc
     5401 gcattcatca agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc
     5461 cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact
     5521 tttgatcgtt tgctgaaaat tgcgccgaaa cgatcactga tcaagcaaat ctgcacgcgc
     5581 agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc
     5641 aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcca gcgccaccat
     5701 ctgggcagtg gttttcactt tcccaatcca ggagacagcc acgctactgc gtttacccaa
     5761 ctccgccatc cattcgcgta acgcagaaat aataatttca cgggcgatca tcgttgctgc
     5821 cggtaaggtc acccaccagc tgtgataatg ctcggttacc agcaccatgg cgatagccac
     5881 gagaacttta tctgctacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg
     5941 gcgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc
     6001 ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat
     6061 gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct
     6121 gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag
     6181 atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg
     6241 ttacgtaaac cttgcaaacc gcccatatat ttcaacaaca tttgccgacg ttttggcccg
     6301 acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc
     6361 ccgccaatcg cgtgatcgtg tgattcatcg cgaatatgct ggataacatg cagtgcggga
     6421 gaatctggcg gcaaactgaa tccctcacct tccggctcaa agaacagcgt ttccagccca
     6481 gccttacgat ctgctccttt ggcaacgcct agtagcagcg gatgattttt atcccatgag
     6541 acatccagtt cggcgaaaac atttttcgcc tgcgcaagtt ggcctttgcc gccgtcgata
     6601 aggatcacat ccgggatctt actctcgtcg atggctttac cataacgccg acgcagcacc
     6661 tgattcatcg ccgcataatc atcgcccggc gtgatgccag taatgttata gcgtcgatac
     6721 tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttccccc
     6781 atggtatggc tgatgtcaaa gcattccatc cgcttcactt ccggcaattt caacacgctg
     6841 gcaagcgcgg tcagtcgctg gtgaacggta gattgctgcg aaagtttgct ggttaaggcc
     6901 gtcgccgcat tggtgcgcgc gagtttcaga taacgcgccc gatcgccgcg aggtttggtt
     6961 tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg
     7021 ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa
     7081 tagaactgac ctacgaaggt ttccaccacc tcgctcagtt ccgtaccgcc aggcactttc
     7141 gggaaatagc tgcggctgcc gagcacttta ccctgacgaa tgaacaatac gtggacacaa
     7201 gccatgcccg catcgaacgc cacaccaata acgtcgaggt cgtcgccggt attggacaca
     7261 aactgttttt cggtgacgcg tcgcaccgcc tgaatttggt cgcgaatacg tgccgcttct
     7321 tcaaactcca gattctggct ggcagtttcc atacgactaa tgagttgcgt aagcacctga
     7381 tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta ttcttcttca
     7441 ctcaccagtc cttcaacgca cggtcccaga cagcgcccta tctgatattg cagacacgga
     7501 cgcgagcgat tgcgataaac actattttcg cactggcgaa tggggaatat cttttgcagt
     7561 agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcaccttta
     7621 gcatgcttcg caccacgatg catcgccaga cgcggatggg tatcgccact caggaagata
     7681 aagggataag atttatcatc gcgtagcaaa acgttgtaac gcggctgata gagtttgatg
     7741 tagttgtgtt ccagcaacag cgcttcggtt tctgtatgag taaccgttac atcaatttgc
     7801 tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctacg gaaatagctg
     7861 gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg
     7921 tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt tgcgtcaaac
     7981 tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt
     8041 gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt
     8101 tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttgg
     8161 tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt
     8221 tttctggttc gatctggctt aacgccattt gttgagcgat gtcagaagca atgtaacgct
     8281 gccctgaata gacagaacgg atcgcactca caacttcctg cggagccgcg cctttgctga
     8341 ggtagcccgc agcaccggcc tgcatgactt tcgctggtaa agggttttct gtatggacgg
     8401 taagcatgat gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaaggc
     8461 cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt gcccggcacc
     8521 atttaacggc gtcttcaccg cacgatgcct ctccgacgac ttttataccc tttatatctt
     8581 ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga
     8641 tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta
     8701 gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt
     8761 tttgtaaatt cgattgtcca cagttatggt cacgcccgtc accaacggct tccgggatcg
     8821 tttcactgta agccctgcga tgaaatgtga caaaaattga ctttattcag caaaaatgaa
     8881 aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaaacattca
     8941 tg
//
LOCUS       DAZGTJ010000150         8392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000150 DAZGTJ010000000
VERSION     DAZGTJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.150"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(116..322)
                     /locus_tag="ACHK8E_004764"
     CDS             complement(116..322)
                     /locus_tag="ACHK8E_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP0346920.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            635..724
                     /gene="kdpF"
                     /locus_tag="ACHK8E_004765"
     CDS             635..724
                     /gene="kdpF"
                     /locus_tag="ACHK8E_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP0346921.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            724..2397
                     /gene="kdpA"
                     /locus_tag="ACHK8E_004766"
     CDS             724..2397
                     /gene="kdpA"
                     /locus_tag="ACHK8E_004766"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP0346922.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2420..4468
                     /gene="kdpB"
                     /locus_tag="ACHK8E_004767"
     CDS             2420..4468
                     /gene="kdpB"
                     /locus_tag="ACHK8E_004767"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP0346923.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4477..5049
                     /gene="kdpC"
                     /locus_tag="ACHK8E_004768"
     CDS             4477..5049
                     /gene="kdpC"
                     /locus_tag="ACHK8E_004768"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP0346924.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5042..7726
                     /gene="kdpD"
                     /locus_tag="ACHK8E_004769"
     CDS             5042..7726
                     /gene="kdpD"
                     /locus_tag="ACHK8E_004769"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP0346925.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7723..>8392
                     /gene="kdpE"
                     /locus_tag="ACHK8E_004770"
     CDS             7723..>8392
                     /gene="kdpE"
                     /locus_tag="ACHK8E_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP0346926.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRF"
BASE COUNT         1736 a         2185 c         2462 g         2009 t
ORIGIN      
        1 atatatgttt cgattggtat aacggtgtct aaatattcct ctgaacatct tcctgataca
       61 aaaaaaaccg ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt
      121 aaaaatcacc agtcgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg
      181 accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag
      241 gcagcgccag aacgcccgct gcaaccgcat aagtacggcg taaaaagata agccatgcag
      301 gatattctct gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca
      361 ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta
      421 ctcatccgac cacttatttt tgcttattga tggtttattt acattcatac cgtaattaag
      481 ttacacaaag gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat
      541 acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga attctactct
      601 tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt
      661 gttgctggtg tttttattac tgggttatct ggtttatgcc ctgatcaatg cggaggcgtt
      721 ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtgtt aatggtgctg
      781 gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg acattcctct tcccggtacg
      841 acgggcgttg agcgcgtact ttttcgcgcg cttggcgtct ctgaccgcga gatgaactgg
      901 aagcagtatc tctcagccat tcttggcctg aatatactgg ggctggcggt gctgtttttt
      961 atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccagg actgtcgtgg
     1021 gatctggcgc tgaataccgc cgtcagcttt gtcacaaata ccaactggca atcttacagc
     1081 ggtgaaacca cgttgagcta tttcagccag atggcgggct taacggtgca aaactttctt
     1141 tctgccgcca gcgggattgc ggtgattttt gccctcatac gtgcatttac ccgccagagc
     1201 atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc
     1261 cctgtggcgt tgttgatcgc attgtttttt attcaacaag gtgcgctgca aaactttctg
     1321 ccctatcagg cggtgaatac cgttgaagga gcgcaacagc tgttacccat ggggcctgta
     1381 gcttctcagg aagcgatcaa gatgctcggc actaacggcg gtggcttctt taatgccaac
     1441 tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc
     1501 ttcttgatcc caacagcgct gtgctttgcc tttggcgaag tggcgggcga tcgccgtcag
     1561 gggcgcatgt tgctgtgggc gatgtcagtg atttttatca tctgcacagg cgtggtgatg
     1621 tgggcagaag ttcagggtaa tccgcatctg ctggcactgg gcgcggacag cagcatcaat
     1681 atggaaggta aagagagtcg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact
     1741 acggcggctt cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tcttggtggc
     1801 atggtgccga tgtggctgat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt
     1861 ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgctgggct gatgattggt
     1921 cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact gaccgcgctg
     1981 gcgattctgg tcaccccaac gctggcgctg atgggcgcgg cgctggcaat gatgaccgac
     2041 gccggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc
     2101 gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctcca
     2161 ttctggaact gtttgctggc gttctgcatg tttgtcggtc gcttcggggt aattatcccg
     2221 gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc agcccgccag ctccggcacg
     2281 ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc
     2341 gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga
     2401 tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt
     2461 cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgtaatcc
     2521 agtgatgttt atcgtctgga tcggcagtct gttaaccacc tttattagca ttgcaatggc
     2581 aagcggcgcg atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt
     2641 caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaagc
     2701 caatagtctg aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata
     2761 tggcgctgcg gcggacaaag ttcccgcaga tcaactgcgt aaaggcgata tcgtactggt
     2821 agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga
     2881 tgaaagcgcc atcaccgggg aatcggctcc ggtgatccgt gaatccggcg gcgattttgc
     2941 ctccgtcact ggcggcacgc gtattctttc tgactggctg gtgattgagt gcagcgttaa
     3001 ccccggcgag acatttctgg atcggatgat cgcgatggtg gaaggcgcgc agcgacgcaa
     3061 aacgccgaac gagattgccc tgaccatcct gctgattgcc ctgactatcg tctttttact
     3121 ggcaaccgcc acgctgtggc cgttttccgc atggggtggt aatgcagtca gcgtaacggt
     3181 actggtggcg ctgttggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat
     3241 cggcgtcgcc gggatgagcc ggatgttggg cgcgaatgtg attgccacca gcgggcgtgc
     3301 agttgaagcg gcgggcgacg tggacgtcct gctgctggat aaaactggca ccatcaccct
     3361 cggtaaccgt caggcatcag agtttatccc tgcgcagggc gtagaggaaa aaacgctggc
     3421 cgacgcagca caactggctt cgctggccga cgaaacgccg gaaggccgca gtattgtgat
     3481 cctcgccaag cagcgcttta acctgcgcga gcgcgatgtg cagtcgcttc acgccacttt
     3541 tgtaccgttt actgcgcaaa gtcgaatgag cgggatcaat atcgacaacc gcatgatccg
     3601 taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggtggtc acttccctgt
     3661 cgatgttgat caaaaagtcg atcaggttgc gcgtcagggg gccacgccgc tggtggtggt
     3721 ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa
     3781 agagcgcttc gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa
     3841 ccgtctgact gccgccgcca ttgctgcgga agcgggtgtc gatgattttc tcgccgaagc
     3901 gacaccggag gccaagctgg cgttaattcg tcagtatcag gcggaaggcc gtttggtggc
     3961 gatgaccggc gacggcacca atgatgctcc ggcgctggcg caggcagatg tcgcggtagc
     4021 gatgaactcc ggtactcagg cggcgaaaga agcgggcaat atggtggatc tcgactctaa
     4081 cccgaccaag ttgatcgagg tggtgcatat tggcaaacag atgctgatga cccgtggctc
     4141 gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc
     4201 gttcgcggca acgtatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga
     4261 ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcccct
     4321 ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt
     4381 atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt
     4441 actgctgacc gtttgcggtc tggtgtgagg tttaccatgc gtggattacg tccggcatta
     4501 tcaacatttc tctttttgtt actgattact ggcggtgttt acccgctgct gaccaccgcg
     4561 ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgattcgcga aggtgatacg
     4621 gtgcgcggct cggcattgat cgggcagaat tttactggca acggctattt tcatggtcgc
     4681 ccgtcggcaa cggcagaaat gccctataat ccacaggctt ctggcgggag caatctggcg
     4741 gttagtaacc cggagctgga taaacaaatc gctgcacgcg ttgccgcatt acgggccgct
     4801 aacccggatg ccagcgcgaa cgttccggtt gaactggtga cggcgtcggc aagcgggctg
     4861 gacaataaca tcaccccgca agcggcggcc tggcagatcc cacgcgtggc gaaagcgcgt
     4921 aatctcagcg ttgaacagct cacgcagctg atcgcaaaat atagccaaca accgctggtg
     4981 aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcgct ggataagctt
     5041 gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc
     5101 gcatcggggg aagctgaaag ttttcttcgg tgcctgcgca ggcgtcggca agacctgggc
     5161 aatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt
     5221 agaaacccac gggcggaaag ataccgccgc aatgctggaa gggctggctg ttctgccgtt
     5281 aaaacgccag gcgtaccgtg ggcggcacat cagcgagttt gatctcgatg ccgccctcgc
     5341 ccgccgtccg gcactgatct taatggacga actggcgcac agtaatgcgc caggttctcg
     5401 tcatcccaaa cgctggcagg acatcgaaga gctgctggaa gctggcattg atgttttcac
     5461 taccgtcaac gttcaacatc tggaaagcct gaatgatgtg gtcagcggcg tcaccggaat
     5521 tcaggttcgg gaaaccgtgc ccgatccttt ttttgatgcc gccgacgacg tagtgctggt
     5581 ggacttgccc ccggacgatc tgcgccagcg actgaaagaa ggcaaagtct atattgccgg
     5641 gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga
     5701 actggcactg cgccgtactg ccgatcgcgt agatgagcaa atgcgcgcct ggcgggggca
     5761 tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc cttttatgca tcggccataa
     5821 cactggtagt gagaaactgg tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt
     5881 ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg
     5941 ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg caacactttc
     6001 tgatccagcg gaagagaaag cggtagtgcg ttacgcccgt gagcataatc tcggcaagat
     6061 tattctcggt cgcccagcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact
     6121 ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg
     6181 cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca
     6241 gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct
     6301 gatggcgttt gatgccgcca acctggtgat gctgtatcta cttggcgtgg tggtggtggc
     6361 gctattttat ggacgctggc cttcggtggt tgctaccgtc attaatgtgg tgagtttcga
     6421 tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac
     6481 cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta
     6541 tcaggcgcgg gtagcccgtt accgcgaaca acgcacacgg cacttatatg aaatgtccaa
     6601 agctctggct gtgggccgca gttcgcagga tatcgccgcc accagcgaac agtttattgc
     6661 ctccacgttt catgcccgta gccaggtgtt gctgcctgat gacaacggta aattgcagcc
     6721 gttaacacat ccgcaaggaa tgacgccgtg ggacgatgcc atcgcgcagt ggagttttga
     6781 taaaggtctg cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt
     6841 gccgctaaaa agcggcgaga aaacctacgg actggtggtg gtggaaccgg ggaatctgcg
     6901 ccagttgatg atcccggaac agcagcgcct gctggagacg tttacgctgt tagtcgccaa
     6961 tgcgcttgag cggctgacgc tgaccgccag cgaagaacag gcgcggatgg caagcgaacg
     7021 tgaacagatc cgcaacgctc tgctggcggc gctttcgcat gatttacgca cgccgctcac
     7081 ggtgctgttc ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcgcccca
     7141 cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac acgacccgac tggtgaataa
     7201 tctgctcgat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac
     7261 gctggaagaa gtggtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcctat
     7321 caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccgc tctttgaacg
     7381 ggtgctgatt aatctgctgg agaacgcggt gaaatacgcg ggtgcgcagg ccgaaattgg
     7441 tatcgatgcc cacgttgagg gcgagaagct acaactggat gtctgggata acggccccgg
     7501 tcttccgcca ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc
     7561 ggcggtaccg ggtgtagggc ttgggctggc aatttgtcgg gcgattgtgg atgtacacgg
     7621 cggcaccatt accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc
     7681 ccagcaaacc gctcctgaac ttgaagaatt tcatgaggat atgtgacaaa cgttctgatt
     7741 gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaagg cgacggtatg
     7801 cgcgtctttg aggccgaaac gctgcaacgc ggcttgctgg aagcggctac ccgtaagcca
     7861 gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac
     7921 ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgtagcga agagagcgac
     7981 aaaatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc
     8041 gaattgcagg cccgtctgcg cgtcgcttta cgtcgccact ccgccaccgc cgcgcccgat
     8101 ccgctggtga aattttccga tgttaccgtc gatttagcag ctcgtgtgat ccatcggggc
     8161 gaggaagagg tgcatctcac cccgattgag ttccgcctgc tggcagtgtt gcttaataat
     8221 gcgggaaaag tgctcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc
     8281 gaacacagtc attatttgcg tatttatatg gggcatctgc gacaaaaact ggaacaggac
     8341 ccggcccggc ctcgccattt cattactgaa accggtattg ggtatcggtt ta
//
LOCUS       DAZGTJ010000151         8229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000151 DAZGTJ010000000
VERSION     DAZGTJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.151"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..4524
                     /locus_tag="ACHK8E_004771"
     CDS             <1..4524
                     /locus_tag="ACHK8E_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP0346927.1"
                     /translation="LDNYFGRYDVSNDGKITSGGDFMTEAQRVRRIFKDGRYVDAEYP
                     VRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARM
                     AGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKE
                     AEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKIRHEIIHHAMEHVVTP
                     AEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVA
                     AWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQY
                     SRSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAPNKELV
                     MPAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEAKDRRG
                     APVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWLLSAGL
                     QLPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVRQMQDKFQVLKAV
                     QENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLANYKISQADLD
                     EYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVD
                     DMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRE
                     SRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDR
                     PDTMRTIAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRA
                     MKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSD
                     GKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWF
                     NMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHAR
                     DAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEG
                     PLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNL
                     VIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAF
                     SPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLG
                     RRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTGRFISKTTDAA
                     VKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAF
                     IDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVD
                     RFNREYNKKVGVE"
     gene            4884..6128
                     /locus_tag="ACHK8E_004772"
     CDS             4884..6128
                     /locus_tag="ACHK8E_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0346928.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
     gene            6215..6688
                     /locus_tag="ACHK8E_004773"
     CDS             6215..6688
                     /locus_tag="ACHK8E_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346929.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            7059..>8229
                     /locus_tag="ACHK8E_004774"
     CDS             7059..>8229
                     /locus_tag="ACHK8E_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0346930.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVR"
BASE COUNT         2121 a         1734 c         2453 g         1921 t
ORIGIN      
        1 ctggataatt attttggtcg ttatgacgtt tccaatgacg ggaaaattac gtcaggtggc
       61 gactttatga cggaagcaca gcgtgtcagg cgcatattta aagacggcag gtatgttgat
      121 gctgaatatc cggtacgtca ggaggtttac gacgctttta aaggtgtgat tcaggccatt
      181 aaaaacagtg acatgccgcg tcgttcagcg cttcttgatg aggtgcgctc aaaaccgtac
      241 tggtcaacgg atgttgaaat ggcggcgcgt gcctttgagc gttatgttca ggataaggcg
      301 cgtatggctg gcgtggagaa tgattatctg gtcaatatcc gtaaggcacc tgagcacaac
      361 acagataaca cctgggctta tccgacgaat gcggaactgg atggcggtat tcgtgatgca
      421 ttcgatcacc tgttcagtac gctgaaaacc cgtgagacgg acaagggcgt tgcgttttat
      481 tcccgtaagg gcgtaacccg cacacctgaa ggcaatctca tttcggatgt taaccgtagt
      541 gcggcggaag ccaaaggcag cccggtcccg caggttgaag cggttgcccg tggcgtgatg
      601 agcggcatta aggacagtga cctgaaggtc cgtgtggtga agtcacagaa agaggctgaa
      661 gcgctggcgg gtgaatcgtt cgacggttac ggcagggtgc acgcgttcta tcgtccggat
      721 aaacgtgaaa ttgtcctggt ggcggataac atccctgacg ggcggaccgt acgcgagaag
      781 atacgtcacg agataattca ccatgccatg gagcatgttg tcacgccagc ggaatatcag
      841 acgattatca aaaccgtgct gaaaacccgc gacagtgata acgccaccat ccgtgaagcc
      901 tggcgtaagg ttgatgcgtc ctatggtaag gaatcaccgg aagtacaggc gggtgaattt
      961 ctggcacata tggcggagaa acagccgaat aaattcgtgg cggcatggga gcgtgttgtt
     1021 gccctggtca aaggggtact gcgtcgtacg gggttactga agccgacgga actgaacgat
     1081 atcagacttg ttcgcgagac catccgtacg ttaggccagc gtgtgcggga aggttacacg
     1141 ccgcgtgagg atggcgcggg cgcatcgtct cagtactccc gtagtggtaa gccggatccg
     1201 tttaaggctg acgcctatga tgcaaagcag tttgcttcag aagtgcgtcg cattgccgga
     1261 ttacaatcgg ttccacgagg tggagaggtg cgggttggtg acactccgcc agtgttaaga
     1321 gcacttggcg caccaaataa agagctggtt atgccagctc atgtggttca caaggccacc
     1381 agaccagaat tacgtgagca ctatgtttct gttgaaacga tggctgactt accgcaattg
     1441 cttgccgacc cggttgcggt gatgacatca aagacggaac atgactcact ggtaactttg
     1501 attgaggcta aggacagaag aggtgcacct gtcattgttg caattcacat gcgtgctaaa
     1561 ggggcggtag ctgaggttaa taagattgcc agtgtctacg gaaaggacaa caggatcgct
     1621 ttgcaggaac agttggatac ttccttgctg tatctgaata aagaaaaagc cgctgactgg
     1681 cttctttcag ccgggctcca attgcccggg gcgaacacca tcaacggctc ttctggattt
     1741 aaagtactac atcctgaaga tattcgcaag ggggcgtatt actcccgcac cagcagtctg
     1801 acaccggaag agaccattgc atcgcgtttt gttcgccaga tgcaggataa attccaggtg
     1861 ctgaaggcgg tgcaggagaa tatccgtaaa actggcggaa aaatagatga cagcaataac
     1921 gcctatatgg ctgaagaact cttccacggg aaggcggaaa acgacctgaa cgtaatgaag
     1981 gagcgctacg ttcagccgct ggccaaattg ctggcgaact acaaaatctc tcaggccgat
     2041 ctggatgagt acctctacgc ccgtcacgcg ccggaacgta acgcacatat tgcgaaaatc
     2101 aaccctaaaa tgccggacgg cggttcgggg atgaccaacg cggaggcggc ggaaatcatg
     2161 cagcgtgtgc gtaacagcgg caaacaggca cagtatgacc gtctggcagg gattgttgac
     2221 gatatgctgg cccgtcgccg tgagcttatc cgtgaggccg gacttgaaga gaacggtgtg
     2281 gtggatgcct ggcagaacgc ctaccgttac tacgttcccc tgaaaggtca ggatgttgac
     2341 ggtgtggtgt cacttccccg tacaggtaag ggcttcacca tcggcggacg tgaaagcagg
     2401 caggccatgg ggcgtgcatc ccgtgcacag tctccgtcca ctcaggcgat acaggacctg
     2461 agcgaatcgc tgatccgcca tcgcaaaaac gaagtgggta acgccttcct gaaactggtg
     2521 caggataatc ccgacaagga ttactggcag gtattcaccg atgacagacc ggataccatg
     2581 cggacgattg cagagcgcaa ggaccaggaa actggtgaaa cccgtcgcga agttgtcgaa
     2641 cgccctgtac cgatggcaat gatggcagac cgctacttca ccaccaaaaa gaacggcaaa
     2701 acgtactaca tcaaactcca tgacccgcgc ctgatgcgtg cgatgaagaa tatgggaccg
     2761 gaaaccagta acgccgtaat ccgtacgctg gggaaagtta accgcttcct ggcaacggtg
     2821 aacacgtcgt ataacccgga attcctggtc agtaacttca tccgtgacgt gcagacggcg
     2881 gtgatgaacc tgaaggctga gcagggaagg agcgacggta aactgaaagg gctggataac
     2941 ttatccgccc tggctgtagt caaagacagc cgttctgcca tgtcagccgt atacgccagt
     3001 ctgcgtggta aaaccctcac gggcaaaggt gcacagtggc agaaggtgtg gaaagagttt
     3061 gttgaggacg gaggtaaaac cggttggttt aacatgggtg accttgaagg ccagcagaag
     3121 gaaatggatc gccttgtttc gctggcgaag ggaggatgga aaggccagag tatcggtgca
     3181 tggaattcgt tccttaacct tgtcgaggat gccaacggcg cggttgaaaa cgcactgcgt
     3241 ctgtcagctt ataaacacgc ccgtgatgcc ggtttgtcac gccagcaggc ggcgtctctt
     3301 gccaaaaaca tgacggtgaa ctttaaccgt cgtggtgagc agggggcgct gatgaactcg
     3361 ctgtacatgt tcgccaacgc cagtattcag ggaacggcaa atctggtgag aacgctcgga
     3421 catcttaatg gtgagggacc tttactggag cgccttcgct ggaagaatct gaatgttccg
     3481 cagaaaatcg cgcttgccgc tgtgggagca ggttatctgc ttggctcgct taaccgcagt
     3541 gtggcgggtg aggatgatga cggggttaac tggtatgaca aggtgccgtc tcatgtgaaa
     3601 gagcgtaacc tcgtcattat gaaatcggtg ttcgggggca aggccggaga gtactggagt
     3661 attcctctgc cttacgggta caacgttttc ttcctgctcg ggcataccgc tgaaggtgtg
     3721 gcggcgggtg acctgacggc gtcccgtgct gccggtaatg ttgttggtgg tgtgcttggt
     3781 gcattcagcc cgattggcag tgagacgtcg gaaacactgt ccggggcatt gctgaaaaat
     3841 gcagcgccga ccattctgcg tccgtttgcg aaccttgcca tgaatgaaaa cttcatgggg
     3901 gcgcagattt accaggagaa catgccgttt ggtacaccaa aacctgacag ccagctggga
     3961 agacgttcaa cgccagaagc gtacaaggcg tttgcatcct ggctgaatgc gttctcaggt
     4021 ggcagccagt accgtcccgg cgcggtggat gtcacgccgg aatcactgaa atactggatt
     4081 gactatatct ctggtggtac agggcgcttc atttccaaaa ccacggatgc ggcggtgaaa
     4141 tcgctgaatg gtattgatat accggagcag caggtgcctt tcctggggaa aatttcaggt
     4201 gaggtgatgc cgtatgctga ccagcagaag atgtacgacc ggatgacaga ggttgcgcag
     4261 taccacgcag agctgaagag tctgaccggt gcagaaagaa cggcgttcat tgacgagaac
     4321 aacggaaaat tgtcgatgaa cgggcttatg caggataccc ggaagagact gaaggatttg
     4381 cgtaaacagc gtgatgccat ttacgccgac agtactctca gtctggcgca acagtcggcg
     4441 atggtgaaat cggtagagcg ggatatgaaa attgccgtgg atcggtttaa ccgcgagtac
     4501 aacaaaaaag tgggagtgga gtaaagaaca tgctccgtac ggaagtgcgg ggctgatata
     4561 aagtcacgca gggaaatatt gtagatgaac tgaacagtta aattcggata atgttgtgat
     4621 attgatcaga aattacagtg taagcagatg tgaaacagaa tgcctgatga gagtaagttt
     4681 tttctgatgc ctgttccggt ggtgtagtcg tggttctgag tgactacacc aagaataatg
     4741 taagacaaat cctaaaacat taaattaata ttttggggtt attttcggaa tatcctgaat
     4801 aaagccgcaa ttaatcatat ttatttatga gtatgaggtg tagtggctga tttgtataca
     4861 gataatttaa caggatatcc ataatgagta aaaaatttac aaagacactt ctgtcatctt
     4921 ctctggttgt aggtctgttg agtgttagtt cttttacaag tgcaagtgaa tatagctaca
     4981 ctgccttgga tattgtcggt agacacggaa ctacttacac cattgtttat aatagtgatt
     5041 ctgacaccgc ttatgttaaa tggaaaacag tacctgccgg tgctgaaggg attgaaagtg
     5101 gaagtattgt acaaggatta tcaaaaatta atagagatgg gactgtagaa gttactacca
     5161 aggatggcac tagtacatat cgtgtgtatg accgggagtt tcatgaccta atgaattcac
     5221 tggtcaagca gaaaagagaa ggttctcttg gaaagaacga gggtactaaa acagagcttg
     5281 tatctgatgc tgatattcgg aatctttttg gcagaattgc tgcgatctct gaaatggaaa
     5341 ctaatgttgg tctggatgaa tatgggcgag tggcaactag tgcagagagt attaaagaac
     5401 gcgtgtcttt gcagagttat ctggctacag aggctgcaaa tagcgcaatt ggcctagata
     5461 aaaacggtaa gctaactctg aatgagacag atattgtaac gccgttacgt atcaatgacg
     5521 gcgttgtcac tatagcgaaa gatgtggaag aacagaaaga agggctggcg atccagaaga
     5581 aaggactgtc tgagctttca tcccgcacag atatcattga tgcagcaacg gctgcgaata
     5641 cgaataaaat tgattcccga accaccgttg gtgtgaatgc ggacggaacc ttgaccagcg
     5701 ctgatggcgc tacaaaaacg attgctgtta atgacggtct cgttgctctg tccggcagaa
     5761 cagaccgtat tgatgctact gttggtagcg ttgaccgccg ggttactaaa aatacacagg
     5821 ctattcagtc caatactcgc cagttgcagg aacataatgc acgcctgaac agccagcagc
     5881 gtcaaattcg tgagaatcac gaagagatga agcgtgcagc agcacagagt gcagcgctgg
     5941 cggggctttt ccagccgtac agtgtgggga aattcaacgc cactgctgcc ctgggggggt
     6001 acagtgataa acaggctgtt gccgtgggtg ttggttaccg tttcaatgag cagacagcag
     6061 cgaaagcagg tgtggcagcg agtgatggcg atgtgtccta caacgtaggt gtgaactttg
     6121 agttttaatt gctgacagtg acatgaagca tgacggcagg cgtggagttt tgcctgccgt
     6181 tttttctcat gtgagcatga aacaggtgac agcgatgaaa gtgattctgg cgacccgcaa
     6241 ccgttatctg gagtatggcc tgcagcagat gcttgagggg tacaggataa tactggcgag
     6301 ggagtttttt acaccagaga acaggaagag tgtaccggca catgatgagt catgggtgat
     6361 aatctgtgat gccctccttg gcaggctgat gtgctgcatg tttcagggac gccgttatct
     6421 tcagattgat gcggaagatg ttacggggcg cctggagacg tacaggaaaa tccggaatgg
     6481 tgagtgggta cataatacgt atgcgcgacc tctgacgatg tccgagatgg tggtgatgtt
     6541 tggatacgtg taccgggagt caaagccctg ccatcttgcc cgtgaaatgg ggataaacac
     6601 gaagacggtg aacacctttc tgtatatggg acttggtaaa aatggtctta agtacaggag
     6661 tgtgaagcac ctggttggac gtgcgtagcc ggaatgcata ttcgtgagtc gggtaatgag
     6721 agtggaacgg tggcagtgag aatggaacag aaaaaaatca gatgcggaaa gcggaaatac
     6781 acagcgggtg cgatgtgctt catgctgttt gtatggctgg ggggtgtggt gacattctgt
     6841 gtgctggccg ctctgataat gaggtgaaaa cggaagagag tttacttcct taattcgaac
     6901 tttctgataa ctgagaactt gttaaatgcc tgtgggttta tgtatttaaa tcaattggtt
     6961 aatggtgatt ttatctttat cagtcaaagt tcgttttagt atgatgattg tgcccgctgt
     7021 ttattgttgt ccgggagaaa atacagggaa tccaggggat gaacaaaata tactcactca
     7081 gagttaatga cagaaaagaa ctggttgctg taccggaaac ggtggggggc aggaaaaagt
     7141 cgtccggcat tcccggtgcg gggatgcttt cccgtcttct tctggcctca ggagccgttg
     7201 ccggcgttct gttttcttct cctgcgctgg catccgttgt cgggaatact ctgccatggc
     7261 agacctacag ggattttgct gaaaacaaag gggcgtttca tgccggggcc acaaatattc
     7321 ctctttatgg taagaatggg gctgtcagtg ggcggctgga caaggctccg atgatggatt
     7381 tcagtgttgt ggaccagata ctgggggtgg caacactgat ttctccgcaa tatgtggcgg
     7441 gggttaagca taacggcagc tacaatacgg tgcgttttgg ttatgcggac gatacgacat
     7501 atcgccttgt tgaccgtaat gagcactggc gtgattttca tactccccgg ctgaacaaac
     7561 tggtgacaga agttgccccg gtgagcgtca cggatgccgg aaccggcaag ggggtatacc
     7621 agaaccgcag tcgttatccg gtgttttatc gtatggggag tggaacgcag tacaccggag
     7681 ctgcttccgg tgcgctgacc agaatagcgg atgcttacgt ctggaggact ggcgggacgg
     7741 tgggaagtcc ggtcatttct gactggagtc tggtgtcaaa cccgggaaat ctttaccagg
     7801 gtgttaatgg tccgctggcg tcttacggga catcaggaga cagtggctca ccgctttttg
     7861 cctgggatgc ggtgaagaag cagtgggtat ttgttgctgt acttcatggt tatgccggtg
     7921 agaacgggaa gacgaactgg tttacggtga ttcctgcagg ggatgtgaat aacacgataa
     7981 agcaggacag ttcaggcacc gtcgtgccgg cagttgccgg tggtgacatt gtgtggaatt
     8041 acagtaaggg aagcggagag ggaacccttt ctcaggatgg gaaggtatgg aagatgaacg
     8101 gcttcagggg agggagtctg aatgacggga aagatatcac ctttgggggt aagggcacgg
     8161 ttgttctgaa ggatgatgtt gtccaggggg cgggttcgct cacgtttaat ggtgattata
     8221 cggtcagac
//
LOCUS       DAZGTJ010000152         8083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000152 DAZGTJ010000000
VERSION     DAZGTJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.152"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..810
                     /locus_tag="ACHK8E_004775"
     CDS             <1..810
                     /locus_tag="ACHK8E_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0346931.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTVMGYSD
                     NEGRSVPVYTRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESQS"
     gene            810..2093
                     /locus_tag="ACHK8E_004776"
     CDS             810..2093
                     /locus_tag="ACHK8E_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP0346932.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            2106..3320
                     /locus_tag="ACHK8E_004777"
     CDS             2106..3320
                     /locus_tag="ACHK8E_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFP0346933.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNLGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIRSIRYDGRNGLHFYSEYEGLQFEEASSMAWFARFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            3334..3687
                     /locus_tag="ACHK8E_004778"
     CDS             3334..3687
                     /locus_tag="ACHK8E_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0346934.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            4016..4471
                     /gene="tssE"
                     /locus_tag="ACHK8E_004779"
     CDS             4016..4471
                     /gene="tssE"
                     /locus_tag="ACHK8E_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0346935.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            4539..4844
                     /locus_tag="ACHK8E_004780"
     CDS             4539..4844
                     /locus_tag="ACHK8E_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346936.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            4899..5213
                     /locus_tag="ACHK8E_004781"
                     /pseudo
     CDS             4899..5213
                     /locus_tag="ACHK8E_004781"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            5538..5687
                     /locus_tag="ACHK8E_004782"
                     /pseudo
     CDS             5538..5687
                     /locus_tag="ACHK8E_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5862..6263
                     /locus_tag="ACHK8E_004783"
                     /pseudo
     CDS             5862..6263
                     /locus_tag="ACHK8E_004783"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            6245..6469
                     /locus_tag="ACHK8E_004784"
                     /pseudo
     CDS             6245..6469
                     /locus_tag="ACHK8E_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            6472..6758
                     /locus_tag="ACHK8E_004785"
                     /pseudo
     CDS             6472..6758
                     /locus_tag="ACHK8E_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6780..>8083
                     /locus_tag="ACHK8E_004786"
     CDS             6780..>8083
                     /locus_tag="ACHK8E_004786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346937.1"
                     /translation="MKRISVNVLDLYLDSKNPRHDPINNRMEIINYLIQNEKIKVLAK
                     SIAENGTSPLETLAVMENENGHFIALEGNRRVCALTLLNDPDLCPENERAYFRKLSDN
                     SDVIPDAVECVLFENREDANKWIELRHNGEQDGAGLLTWNATQKARHFGSSDNTLALK
                     LLDYAETKGFISKQKRDGILTTASRFLGNPVFRSTMGIRTGRSESNVMIDVSRDEFDK
                     VLERFINDLINGENNVTSRSKKEDWENYAKQLRSEGIAPKGHCEPFALFSTEHSNASG
                     KGSTISKIVKNNQSPNNRKYIIPSDFKVNIPDPILKRVFDELRTLECSEYTLASSVVC
                     RAFLENIYSLAYEKFYGNYPEGMAEHVVMEKIINKIEEDKNSLTKKEKKALEALKRVK
                     TDTTNPLSPKTLGANAHAGFYPDPTALKVHWDNISHILLFML"
BASE COUNT         2213 a         1783 c         2143 g         1944 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa actgctcaat gaagcagtgc ttttctcggc accggaagga
      121 atggcgctga ccagcggtga agatatgcag atgagtgcag cacgaaatgt ggctgtcaac
      181 gccggggggg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagaag
      241 ctggggcttt ttgcccggac cggtcaactt agcctgaaat ccggggaagg ggcagtggat
      301 gttcaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac catttcgtca
      361 gaaagtgata tctcgtttac cggcaagaaa cgcatcacgc tgattggcgg ggggagttac
      421 ctgcggcttg aagccgggaa ggtcgagtat ggtacgatgg caacctgtat gcgtcgggtg
      481 aagcggacga tgacggaggc tgcgcagagt atgccgatga cattcccccg tatgagttct
      541 tcgcatatct actcggctat ttatcggttg ctggacaaac acggcaatat tctggccaat
      601 accccctgtc gcctcaccac tccatctggt cagacagtaa tggggtatag cgataacgaa
      661 ggccgctccg tgccggttta cacccgccag caggaggatg tggaactgca tttttttgtt
      721 aaaaaaccac aacccgaaga aacgatgtgg tttgtggggg aaaccgatac gcagcaactg
      781 gaaaccgaac tcagggagag ccagtcataa tggaaatgcc cgatttaccc gagacaccga
      841 catctgatat tcccactggg gcgaacaacc agccgtggga ctgcaccacg acgggtgtcg
      901 aaatggtcga aaagcagtcg cgtctgccaa ccacggaaaa ccgtcagtac ctgcatataa
      961 aggtggaata tgccatgcgt tttccgcaac tgtcggcaca aatccgccgg aaaggtaaaa
     1021 cttaccagat gagccttaag cagaccgtta tgagtggtct tattcgcgtg gatgaaatcg
     1081 cccgcaatta ctgctggtat tataaggcgg aggtggcgtt tgatatgcgc accacgccgc
     1141 cacgcccttt tctgagcagt acgctgctgg aaaaagacgg tgctggccgt cgccacacga
     1201 ccaatccttt ccctgtgccc ggcaggggca tttaccgccg cccggacgtg attattgtta
     1261 aaaataagga agaccgctgg cccggactgg cggggccgga taccgaaggc aatatgcaca
     1321 gcgataacct ggcccgcctg gtggaggtga agtttccggg agataggctg tatgaagacc
     1381 aatatgaaga gtatatgcag attgtgggca ggaaccgaac ccgcatgacc attctggaga
     1441 ttcacgactg tcgcccggaa gagcaccagt gggttgacca ggtggtcaac gtgacgatga
     1501 aagcctggca gatcagcggc gcgaaatact ggccgctggc tctttacccg ccagtcttta
     1561 tgccccgtcc gccatcaacc cccgttgctg ccagaataga gccctggact tacgctggta
     1621 aagtagtatc tgacctcagt gagggggcgg tgcgtggtgt ggctgaaggc tggaatgccc
     1681 tgtcaaaaga gacgcaactg gtcctgaatt cagcagccag ctggcttaac gacagcggtg
     1741 agtgggttta ccggcaggga agtgaggtct gggtctgggc cagccaaacc ggtaagtcgg
     1801 tgaaatcctg gaccaacacc cagattaagg ccgcatggac agaaatacag cacgtcaccg
     1861 atatgacgct tgatatgctg aaacaggtgg actgggtaca ggttttaacc aacgtcgcga
     1921 aaacggtcgg cgttattctg gttgctgtgg gtgtcggcgg actggtcgta acgctgggta
     1981 tcccggaagc cattattggc gcgtttttac tgattgttcg tctggcaata agcgcctggg
     2041 gaacactggc gacgattctc ggtacgggta cggcggtact ggcagttagt taaacaggag
     2101 caggtatgga attgcatgat tttgagctga actacatcga gaaatggctg ccggaagcct
     2161 ccgtgaaata caaagacggc agtccggcag tcaatctcgg tctgattatc accgtctttt
     2221 ttaaggacgg acatacgcca gaagtccgcc gcaggatggt ggagtgcgtg gatcgcttct
     2281 atggggagtt taaaccctgg ctgaaaaaaa cgatcaccaa aaactgggtt ggcatcactg
     2341 ataataacta cgccaaaaag cggcaggaga tcctcgattc aacgcctgaa gagatttttt
     2401 cctggtatct gagtagcgca gagaaggatt ttttagcccc cgattacagt attttaatta
     2461 tggggaaaag aatttttcat aatgacaata atcgttcagt cattaagtta acactcccgt
     2521 tgtcactgct gaaagaaact gatggaaagc agcgttatga agcctggctg atgtggttgt
     2581 gtgacacctt ttcggtggaa agtggttatg cagggttgtc gtttgcactt ccttatgccc
     2641 gtgagcgaat gttcccctgt gaattcgccc tggcgcagcg tttttccggt gtgatggtgg
     2701 attctcttgg gacgcttgaa ggcggtgaag ccgttgaggg gctgaaaggt gcctgctggt
     2761 acaccattct cggtacgccg tggctggaaa aactgggggg ggcagagaga gtaaaacatc
     2821 gtttagctaa aacgcctgaa attagcctgc taccctataa caatggtgtc gtacttaaag
     2881 ccggtgaact ccccccgcca ctgggagaga tgaaaacaga aggtttatcg ccgctgctgg
     2941 tgaaggtgaa ccaggtcatt cgttccattc gctatgatgg acgcaatggt ctgcattttt
     3001 actcagaata tgaaggactt cagtttgaag aagcgtcttc aatggcatgg tttgcccgtt
     3061 ttgacgaagc cagtgccttg ctcgataaag aagaaatggg gaaatcttgc gcccccgaac
     3121 gcattaaatg ctggaccgat gaaatagcgc cccatgccgg acaatgggcc gcgatggtta
     3181 atggcaccac cgagtacatc cagacccgcg aagggcagaa aatgcccccg tttgaggata
     3241 agcacggtaa gcagcacagg gcttgctgga aactactgaa acgagatgac aatggtagcg
     3301 tttatacact acctgagtaa ggtcgtgtgc tgaatgaccg tgcaggccgg agagaggctg
     3361 gggctttttg cccgcaccgg tcaacttagc ctgaaatccg gggaaggggc agtggatgtt
     3421 caggcgcaaa acggcagcat gcggttgttt gctgagaaga agctgacgct gagctcagcg
     3481 agtgacatct cctttgccgg gaaaaaacgc atcacgctga ttggcggggg aagttaccta
     3541 cggctggagg cggggaaaat agagtacggt acgatggcga cgtatctgcg gcggacgaaa
     3601 cggacgatgg tagcagcccc ggcaacaatg ccgctcccca taccgcttct cctggacctg
     3661 tatccaccac ttaatggcca aatttgaatt gaatgtctcc tcaatgccat caaagtcaac
     3721 gatgctatcg ttcagggagc gtagtcaatg gatgtcacaa aaatgatgca ggtgctacag
     3781 gatttgtttt tatgctgcta aatgtacagc gacttcgtga tatggggttg ttggtgtttt
     3841 ttataataat gctttttacg gaatcaaaaa aacagacaac atattaatta tttgagaaag
     3901 cagcgtcaaa aaggaaaagt taaaatgtcg aatcgatttt ttactccggt aatcttcttt
     3961 ccagtgtgtt gattgaacta tttccaagat taaatgatta tgagaagaag tcgtgatgtc
     4021 tcatcatagc ccctcccttt acgaaacctt atacggaaat tttaccggcg gcctcgacct
     4081 ccatcaggtc tgtgaagaaa atcaggtcat cgtgtcggtg ctcgataaca tgcagcgtat
     4141 tctgaatacc cgggccggaa gccttaaaca tcttccggac tatgggctgc cggatatgac
     4201 aaccattctg cagggaatgc cgggtacggc gcatcaactg atgcgcgtgc tttcagatgt
     4261 tctgctgaag tatgagccac gtattaagcg tgtcgacgtg acgatgcagg aacagactca
     4321 gcctggtgag ttgcattatg tcattgatgc agaacttaaa gatgccgggc tggttcgtta
     4381 cggaacaaca tttatgccgg agggcagagt gttgctgcgc catcttaagc aacagcagtt
     4441 tgtggacaat tcatcttatt atcatgtctg atttttttat ccgggctgcc cctgtgtggg
     4501 tggtccggat gaaaaatata ttttttgtga ttttgattat gaacccgtta tttaacgata
     4561 ttcagatgcg tcttttttat ctgaaccact ctccgtactc ctggcactgg aatgtcagat
     4621 tcagacctca ggaagccgta tatattggca gcgatacctg tcatatcacc attacctgta
     4681 atcagtccgg ttttcattta acccgggacg gacaacgggt attcactgag cgttatatca
     4741 gaaaccttaa tgagctgtta ccagttctga aacggcggtg ggatgtgaca ccggcaatca
     4801 tccgtgcagt ggaatacctt tcccgggtcc cggtatcaca ctgacattct ttccgtaact
     4861 gttaatcatg atttaataca gcaacactga atctgccgat gacttctgaa gtgaaactga
     4921 aaacaggtgg tgacccccgg tcattcccgg attatgctgc cctgcgcgat gaaatcagca
     4981 aactgaccca cccggcacgc ccggatgtgg actggcgtta tgtggaaacg ctctgcctgc
     5041 gcctgtatga acacaatggc gtggaattac agacagcatc ctggtacacc attgcccgga
     5101 tgcatacaac agggttaagt ggcctgaatg aagggctggc tctgattgtg gcgctgaccc
     5161 ggcatcactg gtcagtgatg tggccgctga atacccacgc ccggctggaa attattaccg
     5221 gtctgttcaa ccgtctgcaa aaaacgctcc gggccatgcc accggacgac cgtgacaacc
     5281 tgcccctgct gtatcagacc gaaacattcc tgaaggcact gagtgataca ctggcccggc
     5341 acgaacttaa acagagcagt aaagtggctt taccggaaac catggtgaag ggatatatca
     5401 cccgtctgga aaatcagccg atgcagggcg aagtgtcgtc tccggtaacc cttcctgctc
     5461 gggcactccg gtctgatgct ccggatgtac agggacatca gcccctgccg cactcccgtc
     5521 tggtgtatat tgtttctgaa acaaaagcag agcccacacc ctcccttcaa aaatccccgc
     5581 ccacgtttct gaaaccgttt gtggctggtg tctgtgcggc attgctggcg gtcagcgtgg
     5641 ccattccggg atggcagttt ctgacacagc catcaccggc ggagcagcaa ctcagggcgc
     5701 tgctggcacc actaccggat atgctttcag cagaccagat ggcacagctc agcagaacac
     5761 cctcactgtt aaatcatgcg tcagactgga taacactttc aggtcagcag ataacgcgac
     5821 tgacagaact accgctgaca tacaacctgc agcgtagtgc gcagctgttg cagcaactga
     5881 tggtactttt ccccgataat cctcgtgtac aggaaatggt ggataactgg cagaagagcg
     5941 taaggagtcg tgcgctcccg gaagaggcga tgacgggctg gaatgaaggc atgacccgct
     6001 tacagcagct ggctgaacgc ctgaaccgcc tggatgaaca gcggggaaaa tacatgacgg
     6061 tcagtgaact gaaaacggag gtgtttggca tcatgcaggc ttttaaccgg cacataccgg
     6121 cagaagagca gttacgtcgc tatgatgaag tccgtaatca gaatggcagc gaatctcagc
     6181 agaaactggc gcaggatgct ctgatggaac agttgaaccg ctactggctt ctgagacatg
     6241 gagatgctgg gaatccggcg tgacttgggt tcgccaacca ccggaagacg ccattacgga
     6301 tatggcgtat tttccctttt accggtatgc catggccttt gaaggattca ttccggcatt
     6361 ttcatcttgg tttgcaggta accggtgtgg agtggcaata ctgaccggtg tccgggcgga
     6421 tgaatccctc aactgcttta tggggctggt gtctcagcgc aaactgagag aattacgtgg
     6481 gtaggatcag atgatggtat tggaactgaa ggtgatatgg gaaaatggtt gttattattt
     6541 tgtctgctgt agctcagtct gaatgacaac atatacttga aagaacaaat gaaggccgtc
     6601 tcgaagccga agtaaagggt atttaatttg ggcgaaaaag aacggtcaac agggaacaag
     6661 tacaaacctt acgaagtctg ggtatcggcg ctacagaaat ttcgcgaaag atgaaattta
     6721 cacgttctac tgtgtataaa atattagagg gtaaataata ttttcatatg gagggttaca
     6781 tgaaacgtat ttcagtaaat gttttagatt tatatttaga tagcaaaaat cctcgacacg
     6841 atcctattaa taaccgcatg gagataatta actatctgat tcaaaatgaa aaaattaaag
     6901 tattggctaa aagtattgct gagaatggaa caagtccatt agaaacactt gcggtgatgg
     6961 agaacgaaaa tggacacttt attgcccttg aggggaatag gagagtttgt gcattaactc
     7021 tgcttaatga tcctgattta tgtccagaaa atgagagggc ttatttcaga aaactatctg
     7081 ataatagtga cgtcattcct gatgcagttg aatgcgtttt atttgaaaat agagaggatg
     7141 caaataaatg gattgaattg cgccataatg gagaacaaga cggtgcgggg cttcttactt
     7201 ggaatgcaac tcaaaaagca agacatttcg gttcttctga taatacatta gcgttaaaat
     7261 tattagatta tgctgaaact aaaggattta tttcgaagca aaaacgagat ggcattttga
     7321 caacagcatc gagattctta ggtaatcctg tattcaggtc aactatggga attagaacag
     7381 ggcgttcaga atctaatgtg atgattgatg tttccagaga tgaatttgat aaagttcttg
     7441 aaaggttcat caatgactta attaatggtg aaaataatgt cacatcaaga tcaaagaaag
     7501 aagactggga gaattatgca aaacagctaa gatcggaagg tattgccccc aaaggacact
     7561 gtgaaccatt tgctcttttt agtacggaac atagcaatgc ttctggtaag ggaagtacta
     7621 taagtaaaat agttaaaaat aaccagagcc ccaataatag aaaatatatt attccttctg
     7681 attttaaagt taatatacct gatcctatat taaaaagagt atttgatgag ctcagaacac
     7741 ttgaatgttc agaatacaca ttggcttctt cagttgtatg tagggctttt ctggaaaaca
     7801 tctattcatt agcatacgaa aaattttatg gaaactatcc tgaagggatg gctgaacatg
     7861 tagtaatgga gaaaataata aataagatag aagaagacaa gaatagcctc actaaaaaag
     7921 aaaagaaagc tctggaggct ctgaaaaggg ttaaaactga tacgaccaat ccactatctc
     7981 caaaaacgct aggagcaaat gcccatgcag gcttctaccc agatccaacg gcgttaaaag
     8041 tccactggga taacatttca catatcttat tgtttatgtt gaa
//
LOCUS       DAZGTJ010000153         7857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000153 DAZGTJ010000000
VERSION     DAZGTJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.153"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(292..504)
                     /locus_tag="ACHK8E_004787"
     CDS             complement(292..504)
                     /locus_tag="ACHK8E_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFP0346938.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFQLLSQAFYNLK
                     MFLMMDMLGVGDAININTNKNIRQVC"
     gene            complement(580..1197)
                     /locus_tag="ACHK8E_004788"
     CDS             complement(580..1197)
                     /locus_tag="ACHK8E_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP0346939.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISSIAEAVCQLPKLQEVLKRNEII"
     gene            1464..2963
                     /gene="ansP"
                     /locus_tag="ACHK8E_004789"
     CDS             1464..2963
                     /gene="ansP"
                     /locus_tag="ACHK8E_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP0346940.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3076..4137)
                     /locus_tag="ACHK8E_004790"
     CDS             complement(3076..4137)
                     /locus_tag="ACHK8E_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP0346941.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4379..6481
                     /gene="pqqU"
                     /locus_tag="ACHK8E_004791"
     CDS             4379..6481
                     /gene="pqqU"
                     /locus_tag="ACHK8E_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP0346942.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(6517..7182)
                     /gene="mcbR"
                     /locus_tag="ACHK8E_004792"
     CDS             complement(6517..7182)
                     /gene="mcbR"
                     /locus_tag="ACHK8E_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP0346943.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7380..>7857)
                     /gene="curA"
                     /locus_tag="ACHK8E_004793"
     CDS             complement(7380..>7857)
                     /gene="curA"
                     /locus_tag="ACHK8E_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0346944.1"
                     /translation="RHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKVF
                     DAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYG
                     HRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD"
BASE COUNT         1937 a         1882 c         1887 g         2151 t
ORIGIN      
        1 caaagcactg caccaccacc tggtgcccgg atgatcttgc ttcagtggta agagtacctg
       61 attcattgtt cccaaaatgg tgttttcccc tgcccctcca ataatgtgca gctctcacgt
      121 gataatctat tgattaggtt atattaagaa tcaatatttg aaatacatcc attttcttga
      181 ataaatcaac atgtttattt atcaattgct gttttagctc tgactcatca agcgctccat
      241 atttgtccta cagataatcg cgaataaata cagagaacgc catttatcct tttagcatac
      301 ctgccggata tttttatttg tattaatatt aattgcatct ccaactccga gcatatccat
      361 cattaaaaac attttgagat tatagaatgc ctgtgataac aactgaaaca tctgcacaat
      421 gtgaatagtt agtttctttt cagaggaaat gaaataaaca agctgaatga aagggagagt
      481 gatataaatt aaaaaacata acatgaatct aattaattac gtccatcatt gtggataaag
      541 ttactgaata ataaataaca taatcttctg gtatcccatt caaataattt cattcctttt
      601 taatacttct tgtaatttcg gtaactgaca tacagcttca gcgatcgagc tgatattctg
      661 cgcattatcg tgaaaccatt catgccccgg cccccatgtg cgcatggtgc aaagataaca
      721 gtccaccagc gttagttgct caccaaacgc ataaggttca gcggtcaatt gtgaattcag
      781 ccagatataa agcgatttac gatattcaat aacattattc tttaactgtt cgggtgcgtc
      841 aggagcccaa cgctcagggt aatcggcgaa agtgaatgtt ggatagacat tagcaaccag
      901 ccagaccaat agccgttgaa actgctgacg ttcggcgcgc ccaaccggtg gcgcaaggtc
      961 cgggcgacga tcgagaacca tcaatgcaat cgccgctgtc tccgtcataa tctcgtcatt
     1021 ttccagcgcc agtgtcggaa tctgacacaa cgggttcagg gttttcaata actcgcgtga
     1081 agttccctcg tggtcaaaac cactgacatc aacgaactga taaggaatat cagccagtgt
     1141 tagcatcatc tcactgattg tagtgcccca tccgggtacg ccatgcacct taatcatatg
     1201 cccctcacaa tcttttaaag attaagtcta ggacagcaca caggttaacc agcaaaagta
     1261 tttgtcattt tttcgtcagt tgtatgaatc cccttcagaa ttgtaagggg atgttttgca
     1321 tcgtttatcg ataacgggta ggatgctgcg ccgatagttt tatacccata aagaataatg
     1381 gtgataacta tcatcgccag gatgaataaa cattgttcat ggcaacttat atgacttttt
     1441 cattaaagca atcagggaga gcaatgagta aacacgacac cgacacttca gatcagcacg
     1501 ccgcgaaacg ccgctggctt aatgcccacg aagaggggta tcacaaagcg atgggcaatc
     1561 gccaggtgca gatgatcgcc attggcggcg cgattggtac gggtttgttt ttaggcgcgg
     1621 gagcccgact gcaaatggcg gggccagcac tggcactggt ctatttaatc tgcggcttgt
     1681 tttcgttttt tattctgcgt gcattgggtg agctggtgct acaccgccct tccagtggca
     1741 gttttgtctc ttatgcccgt gagtttttgg gtgagaaagc cgcttatgtt gcaggctgga
     1801 tgtacttcat caactgggcg atgacgggga ttgtcgatat taccgccgtt gcactgtata
     1861 tgcattactg gggcgcattt ggtggtgtac cgcagtgggt ctttgcgctc gctgcgctga
     1921 ccatcgttgg caccatgaat atgattggtg tgaaatggtt tgccgagatg gagttctggt
     1981 ttgcgcttat taaagtgctc gccattgtga cctttttggt cgtgggtaca gtgttcctcg
     2041 gtagtggtca gccgctggat ggcaacacca ctggctttca tttaattacc gataacggcg
     2101 gcttcttccc ccacggtttg ctacctgcgc tggtgttgat tcagggcgta gtgtttgctt
     2161 ttgcctccat tgaaatggtg ggtacagctg ccggagaatg taaagatccg cagaccatgg
     2221 tgcctaaagc gattaacagt gtgatttggc gtattggcct gttttacgtc ggctccgtgg
     2281 tgttgctggt tatgttattg ccgtggagcg cgtatcaggc ggggcaaagt ccgttcgtga
     2341 cgtttttctc taaactgggt gtgccgtaca tcggcagcat tatgaacatt gtggtgctga
     2401 ccgctgccct ctccagcctg aactcaggtc tgtactgcac cggacgtatt ctgcgctcga
     2461 tggcgatggg cggttccgca ccgagtttta tggcgaaaat gagtcgtcag catgtgccgt
     2521 atgccgggat tctggcgaca ctggttgtgt atgtcgtcgg cgtattcctc aactatctgg
     2581 tgccgtcgcg cgtatttgag attgtgttga acttcgcgtc gctgggaatc atcgcttcat
     2641 gggcgtttat catcgtgtgc cagatgcgcc tgcgtaaagc gattaaagaa ggcaaagcag
     2701 cggatgtcag ttttaaactg cctggcgcgc ccttcacttc ctggctgaca ttactgtttt
     2761 tgctgagcgt ccttgtgctg atggcgttcg attacccgaa cgggacttat actatcgcgg
     2821 cgctgccgat tatcggtatt ctgctggtta taggctggtt tggtgtgcgc aaacgcgttg
     2881 ctgaaattca cagcactgcg ccagtcgtcg aggaagatga agaaaaacag gaaattgtgt
     2941 ttaagcctga aacggcgagc taattgcaaa attgcccggc tacgtgccgg gcaaattcaa
     3001 attactgacg aaggtgccgg atacggcgtg ataaaagtca ggcaagtctc tccattttca
     3061 ccctcgccat atctattaca gcgcaatacg aatcacatcg tccggctggg tagcttcctg
     3121 ctgtttagtg gatttttgtt tcacactgac atacagcgtt ttgccatcgg cagacagtgc
     3181 caggctgttc ggatgagtcg gcgtatcgaa cgttttcacc actttatagc ttttcgcgtc
     3241 aatcacactg actttacctg cctgacgatg cgtcacgtag gcttcattac gcgcggggtt
     3301 aaacagcaca gccagtgatt ccggtgccgc aaccttcgcc agaatattgc cattacgggt
     3361 atcgaccact aacacttctg ccgctttaga gtcggtgata aatgcacgct gccttgcggt
     3421 atcaaggctg atgttgataa agaagtgctc tttgccgtca tccagcagct ttttacggct
     3481 gaggatttta ttgtcggcgg tgtcgatggt aatcaattcg ccgtcagcgt tagtggtgta
     3541 aagacgtttg cctttgctat ccagcgctaa accggtactc attttaccgg tgttctggat
     3601 ggcggttttc agtttgatat tctcgccatc aacgacccaa atcacgctct ctttaccaat
     3661 accactgatg taaacggtgt tcgtggcatc gtctgccacc agctcacgcg gttgcagcgg
     3721 gcgcacctct tcagtgcgct tacgatcatc cagcaccaga cggcctttca cttcgcccgt
     3781 tttggcatct atcgccgtga ccgcgctgtt tacagtgtta ccaaaccaca acgtctgagt
     3841 cgtgttattg atggtagcac caaacggctt gagatcgtta tggatcgcct gcgtcacttc
     3901 cagagtaacc ggatcaagac gataaaccac gccgccttta tccagtttgc ggctttgcga
     3961 agtggcgagc cacagcgcgt tttcttgctg gctataagcc atttcgtagg cacctttacc
     4021 taccgcttta cgcagcattt cttctgcggc ctgcgtactg aatgatgaag caacaagcaa
     4081 tgaacctaac agtaatgaac cacgcaggcg cgatgaaaac agatgacgta aatgcatgac
     4141 gactcccttt gatgaaccga tatttttgct gcttcacaat gctccgctaa gcgtaatcat
     4201 cgttacgctc ttgttataaa tgggaatggt aatcattatt ttctcaaatg tggagcattt
     4261 cttgccgtta cgactacaat tttctcaatt acttaacata tcgtgccgtt tcgttttcca
     4321 taaatttaca attttattac catgacggtg ttttcccatt cttaacctgg cttttttcat
     4381 gaagattttt tccgtccgac agaccgtttt tcccgcgctg cttgtccttt cccccaccgt
     4441 ttttgccgct gatgaacaga ccatgattgt cagtgccgcg ccgcaggtga tttcagaact
     4501 ggatacccca gcagccgtga gcgtggtgga tggcgaggag atgcgcctgg caacaccgcg
     4561 cattaacttg tctgaatcac tgactggcgt gcctggtttg caggtacaaa accggcagaa
     4621 ctatgcgcaa gatttacagc tgtcgattcg cggatttggc tcccgctcca cttacggaat
     4681 tcgcggtatt cgcctgtatg ttgacggtat tcccgctacc atgcccgacg gacaagggca
     4741 gacatccaat atcgatttaa gcagtgtgca aaatgtggaa gtgctgcgtg gccccttctc
     4801 tgctctgtat ggcaacgcgt ctggcggtgt aatgaatgtc atcacccaga ccggacaaca
     4861 gccaccaacc attgaagcca gtagttacta cggcagtttt ggcagctggc gctatgggct
     4921 gaaagcaacg ggcgcaacgg gagacggcac acagccaggc gatgtcgatt acaccgtctc
     4981 aaccacgcgc tttacgaccc acggctatcg tgatcatagt ggcgcacaga aaaatttagc
     5041 caatgccaaa ctgggcgttc gcattgatga cgccagcaaa ttaagcctga ttttcaatag
     5101 tgtggatatc aaagcagatg acccaggtgg gctaaccgaa gcagaatgga aggcgaatcc
     5161 acaacaagcg ccccgtgcag aacagtacga cacgcgaaaa accatcaagc aaactcaggc
     5221 tgggttgcgc tatgaacgta gcctgagcgc gcaagatgat atgagtgtaa tgatgtatgc
     5281 cggagagcga gaaacgaccc agtaccagtc aatccccatg tccccgcaac ttaacccgtc
     5341 acatgcgggc ggcgtgatta ccctgcaacg ccattaccag gggatagaca gccgctggac
     5401 acaccgtggt gaattgggcg ttccagtcac gttcacaacc ggcctgaact acgaaaacat
     5461 gagtgaaaac cgcaagggtt acaataactt ccgcctgaat cgcggcgtgc cggaatacgg
     5521 gcaaaaaggt gagttgcgcc gcgacgaacg caatttgatg tggaacgtcg atccctattt
     5581 acaaacccag tggcaactga ccgacaaact ttcgctggat gctggcgtgc gctacagctc
     5641 tgtgtggttt gattccaatg atcattacgt tactccgggt aacggcgacg acagcggtga
     5701 tgccagttat cacaaatggc taccagccgg atcgttaaaa tatgcaatga ccgatgcctg
     5761 gaatgtctat ctggcagccg ggcgcggttt tgaaacgccg acgattaatg agctgtctta
     5821 tcgcgctgat gggcaaagcg gtatgaactt tggtttaaaa ccctccacca acgatacaat
     5881 tgagatcggc agtaaaacgc gtattggtga tgggctgctt agtctcgcat tgttccagac
     5941 cgacaccgat gatgaaattg ttgtcgatag cagtagcggt gggcgtacga cttacaaaaa
     6001 tgccggaaag acccgtcgtc aaggcgctga actggcatgg gatcaacgtt tcgcgggaga
     6061 ttttcgcgta aaagcgtcct ggacctggct tgatgcgacc tatcgcagca atgtttgcaa
     6121 tgaacaggat tgtaacggta accggatgcc agggatcgcc cgtaatatgg gctttgcgtc
     6181 gataggttat gtaccggaag atggctggta tgcaggtacg gaagcgcgtt atatgggcga
     6241 tattatggca gatgatgaaa atacggccaa agcgccgtct tatactctcg tcggcttatt
     6301 caccgggtat aaatacaatt accacaattt aactgtggat ttatttggtc gtgtcgataa
     6361 tttattcgat aaagaatacg ttggctctgt cattgtcaat gagtcaaacg ggcgatatta
     6421 cgaacctgcg cccgggcgaa attatggtgt cggcatgaat attgcgtggc gatttgagta
     6481 attaccttga tgcccggtat ttgccgggca tttaatttaa cgattgtatt gctggtataa
     6541 aatagcaata ttttgttgca taatttcagc aagacaatgt ctgcatccct ctttgtcttt
     6601 tgcttttaac gcggcgagta atagacgata gttttctata cgctcccgta attccgctgg
     6661 attaatcgct tcatagagat aatgtaaacc aggtcccatc ctgacccaca gttgctcaat
     6721 catctcacac aggatgggca tatttgaacg atgataaatc gctaagcgaa atagcctgtt
     6781 cgcattaatg atttgttcca tgtcgccctt ttcttgcgcc tgctgtaatt tctcgagcaa
     6841 ttcctcgagt tccgccaggt cttgcggggt gaggttttca acagccagag taactgccat
     6901 taattccagc tcgtagcgga tccgattgat ttcgtctaat tgacgtttcc ccacttccgg
     6961 aactgtaaat gcttgcgctg gtgcgacaga aagcgcattc accgaaacca gacgtaataa
     7021 tgcttcacgc acaggtgtaa tactcatacc taattgctcc gccagatttt tagtaatcag
     7081 gcgtgcgcca ggttttaatg cgccaatact aagctgatgt ttcaggtcgt tctcaacctg
     7141 cagagtcaaa ctgacatgtt tcattttttc cgttccaggc attttaattc cttcgcgtgt
     7201 tttctgtcca accctgcctg catggcagaa ggagttcacc tggtgtatta aagtgaataa
     7261 tcataaacca ctcacctttg ttgttattag cagcagaaca atgacgtggt tagttaatta
     7321 aagctgaaaa taagcttttt gcctggattt ttatggcggc ttacgccgcc gttgaaactt
     7381 taatcatcaa ccgccacgcg gatcaccact ttgccgaagt ttttaccctt cagcaggccg
     7441 ataaacgtct gtggcgcatt ctctaaaccg tcagtaattt cttcgcggta gtggatttta
     7501 tcctctttca cccattgccc catctccttc tgaaactctt ggatgcggtg accataatcc
     7561 tgagcgataa taaatccttg caagcgaata cgttttttca gcaccgtagc catcagcaga
     7621 ggtaaacgat ccggaccggg tggtagctct gtagcgttat agctgctcac taagccacaa
     7681 acgggaatgc gcgcagatgt attaagtaaa ggtagcactg catcaaatac cttaccgccc
     7741 acgttttcat aatagatatc aatacctttt gggcacgctt tcgccagttg ttcggcaaaa
     7801 tcatccgcgt gatggtcaag acaaacatcg aagcccagca cctcagtagc atggcgg
//
LOCUS       DAZGTJ010000154         7841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000154 DAZGTJ010000000
VERSION     DAZGTJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.154"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..194
                     /locus_tag="ACHK8E_004794"
     CDS             <1..194
                     /locus_tag="ACHK8E_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346945.1"
                     /translation="YSEPVHGIQGYNCYDGGEHTDSDLQAEWPGRETLPQPRLYLVEE
                     CAEEQTKAAPLPVPLVSAQ"
     gene            306..992
                     /locus_tag="ACHK8E_004795"
     CDS             306..992
                     /locus_tag="ACHK8E_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0346946.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(1226..1778)
                     /locus_tag="ACHK8E_004796"
                     /pseudo
     CDS             complement(1226..1778)
                     /locus_tag="ACHK8E_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1896..2813
                     /locus_tag="ACHK8E_004797"
     CDS             1896..2813
                     /locus_tag="ACHK8E_004797"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0346947.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            2846..3721
                     /locus_tag="ACHK8E_004798"
     CDS             2846..3721
                     /locus_tag="ACHK8E_004798"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0346948.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            3770..5242
                     /locus_tag="ACHK8E_004799"
     CDS             3770..5242
                     /locus_tag="ACHK8E_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346949.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            5246..6076
                     /locus_tag="ACHK8E_004800"
     CDS             5246..6076
                     /locus_tag="ACHK8E_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0346950.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            6122..6832
                     /locus_tag="ACHK8E_004801"
     CDS             6122..6832
                     /locus_tag="ACHK8E_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP0346951.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            6845..>7841
                     /locus_tag="ACHK8E_004802"
     CDS             6845..>7841
                     /locus_tag="ACHK8E_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0346952.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVS"
BASE COUNT         2183 a         1356 c         1938 g         2364 t
ORIGIN      
        1 ggtacagcga gccggtacac ggtatacagg gttacaactg ttacgatggc ggagagcata
       61 cggacagtga tctgcaggcc gaatggccgg gcagggaaac acttccacag ccccgactgt
      121 atctggtgga ggagtgcgct gaagaacaga cgaaagccgc acctttaccg gttcctctgg
      181 tctcagccca gtaattaacc tccgggtatc atgtccgtga tgcccgtttt atcaacattt
      241 caacccgaag gggaacacct gttcccctgt ccgggccatg tgttccctgt ttttacggag
      301 aaaacatggc caccatgact gaccatcaga aggagttcat ttcgttgttt aaccggacag
      361 cctgttatca caaacgtcat aaggtgtttg aagactttgt cagctgtagt gtgattgccc
      421 tggaaaaccg gcttcagttc agtgaggccc gggaacagaa gtatctgcga atcgtcagtg
      481 gttatgaaaa gcaggatgtt atccacattg cgcatttgct tgctcatgtc attgagggac
      541 tggagcaggg attctgtgat tttctgggga gtgtgttcat gcaactggaa ctgggggata
      601 aataccggag tcagtttttc acgccctgga gtattgccag catgatggca cagatgcaga
      661 tgggagatgt gaaggcgttg tttgagaaca aaccctttat cacgctcagt gaacctgcct
      721 gtggtgccgg cagtatgatt ctggcaatgg ctgacatcct gaaccgttcg gggtatccgg
      781 cataccacag gatgtgggtg tcggcaacgg atattgaccc gctggcagcc ggcatggcgt
      841 atatacaact gtcgttgtgt ggtgtggccg gagaagtggt gattggtaac tcgctctgta
      901 atgagcgacg ccgcgtactg ctgactcccg ggcattacct gggaaactgg gaccatcgtc
      961 tgcgtcatct tcaggagcag aatgtggcat gatgcagaat gttttatcac tgcgccagtc
     1021 ttcggactgg cgttttgttc aactgccagt tccctgaatg tttgatttgt tcaataaagc
     1081 ctgtttatca ataaattctc tgacgacagg gaatggtatg tttttattag tttttatgtg
     1141 ttggacaaca agacagaata atgatgtctg ttttatgtat acgatatagc cactgattca
     1201 acgcattatc ggctatattg ccagtcttat ttagtgccag attcattgta tcgaaggccg
     1261 ctgccgtcag tagatggctg gtcagttcta tcagacagac catttttacc tgaggataaa
     1321 gtgtctggtt tccactatgt atttggcgag tgaagcttta tcgtgttctt gcgtgtcctg
     1381 tgttcgtcag aacacgccat caatggtcag cagattcaga tcgcaccaga gcggatatgg
     1441 cgtggtgtta tgctagagct gcgctgtttg agtgaacacg cagcgtatgg ctccgcttcc
     1501 tggccgctgg cgggcatgga gcacggcgct actgcctggc agcatgatgt ccagccggtt
     1561 cacaatctga tgaaggttcc ttacgctcat gggccatact gataattcac cagaccatca
     1621 tttccagagg aagacgccgt ttgtgtagcg tgacagtgtc ggactcttca gggctgtggg
     1681 taatcagttc cagggcaaga tagtcgcaga gggttacgca gagaatagta actggacact
     1741 ggatcgatca accgatcctt aactgagcgg catgacagct ttctgatggc ttttttttga
     1801 taatcttcac attgtgcttc ttttctgttg gataatcttt gtacttgatg gtgtactagg
     1861 tatcccatta tgcagttaag tgaaggtgat taaaaatgca atgtgaattt aaaggggtta
     1921 tatcagcatt acccactcct tatgaccaat cccagcaaat cgatatggag agtcttcgaa
     1981 aactgatccg ttttaatatc gaacaaaata ttaagggatt gtatgttgga ggctctactg
     2041 gagaagcatt tcttcagaat gttgcagagc gagaaaaaat tcttgaaaca gtagccgatg
     2101 aatcagatgg aagactaaca cttatagcgc atgtgggcgg aatcagtacg gcagaaagcg
     2161 aggtacttgc aaaagctgca aaaaaatacg gatatcatgc tatatctgct gttactccat
     2221 tttattatcc tttttcattt gaagagcatt gtatccatta caggaagatt attgattctg
     2281 cagatggact tccgatggta gtttataata taccggctct tagcggagtg cgtttttctt
     2341 tagatcagat aaatgagctt gtcacaatac ccggggtttg tgcattaaaa cagacatccg
     2401 gtgatttatt ccagatggaa caaatcaaaa ggaatcatcc agagcttgtg ttatataatg
     2461 gttacgatga aatatttgca tcagggttaa ttgcaggtgc agatggggga atcggtagta
     2521 catataatat aatggggtgg cgttatcttg aaatatttga agcagtcaaa aataatgatg
     2581 tgattaaggc gaaggaaatg caggtggcct gtaaccaggt tattgatact cttattcaga
     2641 gtggtgtcct ggcaggtata aaaactcttc tttactatat ggggattata aatactcctg
     2701 tatgcagaag tcctttttct ccggtaaagg aaaagaatct ggatgtatta tcaaaacttg
     2761 cagagcgact gctcgaagaa catgacagaa ataaaaaaat gaaaataatc tgaataattg
     2821 caagattgtt atataggtaa aatgaatgat aacactggct gttgatatag gaggaacgaa
     2881 aatttcagcg gcgttgatat ctgatgacgg ttcatttttg ctgaaaaaac agatatcaac
     2941 acctcatgaa agatgtcctg acgaaatgac aggggcttta cgtctactgg tttctgaaat
     3001 gaagggaaca gcggagcgat ttgcagtagc atcaacaggg attattaata atggagtatt
     3061 gacagctttg aatccagaca atcttggagg gctgaaagag tatcctctga aaaatataat
     3121 ggaagatata actggtttaa atggaagtgt gataaatgat gcccaggcag ccgcatgggc
     3181 agaatataca gttttgccaa aagaaatttg tgatatggtt tttataaccg tttctacggg
     3241 ggttggaggg ggaattgtag ttaacagaaa attattaaca ggtgtttctg gattagccgg
     3301 acatgttggg catatattgt ccggagtaac ggatacagaa tgtggatgtg gtcgtcgggg
     3361 atgcgttgaa gcggtttcat ctggtcgggc catcatgggg gcagcaaaaa atgaacttgc
     3421 aggatatagc accaaatata tttttgaatt agcgagacag ggatataagg aagccgaatt
     3481 tttgacagag cgttctgcca gtactattgc tgaattaata gtaagtttaa aattactgct
     3541 tgattgtcag gttgttgttg tcggaggaag tgttggactt gctgatgggt atattcagaa
     3601 agtgtcgaaa catctttcca tatattctga aatttgtaat gtaatgctgt ttccggcata
     3661 tttcagaagt gactctggtc ttattggtgc aactctttgg gaccgggatt gtattactta
     3721 aagtaatact ccagtgtatt ataaatccgc tattattgtg aggtttaata tggactcctg
     3781 tattcagaaa aagcaggttt ggtataagca tctttcaccc agacagtgga agatattcgg
     3841 tgcggcatgg acaggttatt tacttgatgg ttttgatttc gttctgatat ctctggttct
     3901 tacagaggtt cagcatgaat tcggcttgac gacaatagaa gcggcaagcc ttatttccgc
     3961 tgcttttatt tcccgatggt ttggggggct tgctattggc gctttatcgg acaagatggg
     4021 gcgcaggatg gcaatggttt taagtatagt gctattctct ctcggaaccc ttgcctgtgg
     4081 tctggctcct ggatatgcag tgatgtttat tgctcgcatt gttattgggc ttggtatggc
     4141 aggtgaatat ggctcaagtg taacatatgt tatcgaaagc tggcctgttc atctcagaaa
     4201 caaggcaagt ggttttttaa tttctggatt ttctattggg ggaggtttag ctgcacaggt
     4261 ttatagcatt gtagtaccac tatggggctg gcgttctctt ttctttgtcg gcatgcttcc
     4321 aatattattt gctttttatc ttcgcaagaa tcttccggag tctgatgact ggcaaaaaag
     4381 gcaacaagag aataaacccg tcagaacaat ggtggatatt ctgtacagag aaaagaataa
     4441 atacataaat attcttctgt cttgtattgc atttgcatgt ctatatgttt gtttttcagg
     4501 cgtcactgca aacgctgctt taatcacagt tatggcattg tgctgtgcgg ctgtttttat
     4561 cagttttata tatcagggta tggggaaaag atggccaaca ggcattatgt tgatgcttgt
     4621 ggttatgttc tgtttcctct acggatggcc actgcaggct tttcttccta catggcttaa
     4681 ggttgatatg cagtattcac cagaaactgt tgctctcata tttatgctgg caggttttgg
     4741 ctctgcagct ggaagttgta taggtggttt tatgggggac tggcttggaa cccgaaaagc
     4801 atatgttata tcacttctta tcggtcagct tgttattatc cccgtttttc ttgtggaccg
     4861 ggactatgta tggttacttg gccttctgat ctttactcag caggtttttg gtcaggggat
     4921 tggtgctctg gtgccgaaaa taatcagtgg ttattttaat gtggagcagc gtgcggcagg
     4981 actggggttt atttataatg taggttcact tggtggagca tgtgcgccta ttcttggtgc
     5041 agttgttgcg tctcatacaa gtcttggtac agcaatgtgt tctcttgctt ttatcttaac
     5101 atttgttgtt cttgtcctta ttggttttga tatgccatca cgtgtacaga gatggatcca
     5161 tccagaagct gcgcttgaat atgatactgt cgatggaaaa cctttttatg gagcaagaaa
     5221 aaagaatgtt gcaggagaat aaaggatgct atcagggaat aaaaaaatga ttcattacag
     5281 ggagaatata tattctcttg ataattatga gcgttacata actgacaatt cgctatttga
     5341 gaatactgac gatggcataa tatatacagg tattgtgccc cttattacag ttgttgagcc
     5401 tgttgtatct aatcaacata gtgtgctgat tatacctgga ggtggataca ggaggattgt
     5461 ttatgaaaga gagggacttg atgtaggcag atggatagca ggtcagggat atacggcatt
     5521 tattctttct tacagaatgc cctccgttaa tggttattat tcctttgcgg atgcgaagaa
     5581 tgccctgaac tttatttctt actgggccgg aatgtcggag agaacattta agagtgttgg
     5641 atgtatgggg ttttcggcag gtggtcatct tgctggatat attactcatc tccattatga
     5701 ggaagagact tctgtgcgtc ctgactggat ggctctgata tatccagtta tatctcttga
     5761 caggagtatt gctcatcagg gatgccggaa acgtcttctg gatgcaggtg aaagtgaata
     5821 ttcagccagt cttgaaaata gtgttacaga gtatatgcca ccagtcttta ttgtgcatgc
     5881 aacagatgat aaggatgttc cagttattca ctctctgaga tattttaact ctatccggga
     5941 taagggacat catgcagaag ctcatttttt tgataaggga gggcatggat ttggtgtatc
     6001 cggaaaaaat ggatatccga tagaaaaatg gcaaagcctg cttctggaat ggcttgcgga
     6061 acataagttt cattgagaaa tcagattaca agtgataaat atatttaatg atgaggcatt
     6121 tatgaaaaaa tattatccac tattaactgt gattcttctt ggtttacctt tgtgctctca
     6181 ggcattaact cttaatctac gtggaggata cagaagcgcc agtcacgctt atgaaacgtt
     6241 gattcaggtt agtcaggggt ggaataatgg ttggtgggca agttttgagt caaatacctg
     6301 gaatacaatt cacaacaatg aaaatgaatt gtttgcacta aattataata aaatagatat
     6361 aaataaaaca tataaaatag atgacaaatg ggctctaatc ccagggattc agacacaatg
     6421 gatgtcagca ggaagtcagt ataaccctta tctgaaaatt tcgtatcagg caacgccaga
     6481 tcttaatctg gccttacgct atcgctatga ctggaaagct ttccgacaga cggatttaga
     6541 tggcaacagg gcgcgtaatg atcagcatca gtttgatggt tatgtgacat acaaagttaa
     6601 tgattcatgg ttgtttgcat ggcagaccac ggtatatacg aaggttaata atttcaaata
     6661 tgggaatcat aaaaaaacag caactgaaaa tgcatttgtt ctgcaatata aaatgtcacc
     6721 ggtgtttacc ccatatatag aatatgatta cctggataaa caaggggtgt ataaagggaa
     6781 agataataaa catgaaaata gttaccgtgt cggtgttttg attaattttt aaaaggtggt
     6841 tacaatgaaa gtgaaaaact ttatatatct tcccttctgc ttgtttattg gaacgtcggt
     6901 agccggagct ttaccggaaa ttcctgaacc atttaaatat ggtgtaggag ggatcgataa
     6961 tggtaaaata tatataggac tgggaagtct gggaaataac tggtatatga tagatacaaa
     7021 ccagagtgaa aaaaaatgga ctaagatagc acaatggccg acagttccca gagagcaggc
     7081 aacagctact attattgatg gtaagatcta tgtatttgga ggaattggaa aagataaaag
     7141 tggagtcata accttacaaa aagatgtcta ttcctatgat attgctaaag ataaatggga
     7201 aaaattgatg acccgtcccc ctgtatctct tgccggtcat gtttctttta tccataatgg
     7261 tcatgcggta tctactggtg gtgtaaatga gaatattttt aatggatact tttcagatgt
     7321 cgagttatca aaagggaatt ccgcgctgac agagaaagtc aacagggatt attttagtaa
     7381 acctgttgat gattattttt taaataatca tatgatttct tatgacccgt caaaaaacca
     7441 gtggaagaac ctgggaacga ctccatttcc gggaactgct ggttcatcgg tgatttttgc
     7501 agaacagcaa atttatatac tgggagggga gagaaaaccg ggccttcgtt ctgttagatc
     7561 ctggacgggg gagctttcgc atgatcgaat taaatggtct gaacttccac cagtagcttc
     7621 acccgagggg gtgtcaggag catatgcctc tgttattgat ggaaatatct ttctggccgg
     7681 tggtgcatat ttcccagggg cggcagaaaa gtatagcaat ggtgaatact ggtctcataa
     7741 aggactggat aaagcctatt caaaagagat atatcagcta ataaaaaatg actggaaaaa
     7801 agttggttct ttgccggaag gacttgctta tggtgtaagc c
//
LOCUS       DAZGTJ010000155         7563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000155 DAZGTJ010000000
VERSION     DAZGTJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.155"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(31..107)
                     /locus_tag="ACHK8E_004803"
     tRNA            complement(31..107)
                     /locus_tag="ACHK8E_004803"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Arg,seq:acg)
     gene            complement(306..382)
                     /locus_tag="ACHK8E_004804"
     tRNA            complement(306..382)
                     /locus_tag="ACHK8E_004804"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(346..348),aa:Arg,seq:acg)
     gene            complement(386..478)
                     /locus_tag="ACHK8E_004805"
     tRNA            complement(386..478)
                     /locus_tag="ACHK8E_004805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Ser,seq:gct)
     gene            complement(794..979)
                     /gene="csrA"
                     /locus_tag="ACHK8E_004806"
     CDS             complement(794..979)
                     /gene="csrA"
                     /locus_tag="ACHK8E_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP0346953.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1214..3844)
                     /gene="alaS"
                     /locus_tag="ACHK8E_004807"
     CDS             complement(1214..3844)
                     /gene="alaS"
                     /locus_tag="ACHK8E_004807"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP0346954.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3972..4472)
                     /gene="recX"
                     /locus_tag="ACHK8E_004808"
     CDS             complement(3972..4472)
                     /gene="recX"
                     /locus_tag="ACHK8E_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP0346955.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4541..5602)
                     /gene="recA"
                     /locus_tag="ACHK8E_004809"
     CDS             complement(4541..5602)
                     /gene="recA"
                     /locus_tag="ACHK8E_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0346956.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5682..6179)
                     /gene="pncC"
                     /locus_tag="ACHK8E_004810"
     CDS             complement(5682..6179)
                     /gene="pncC"
                     /locus_tag="ACHK8E_004810"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP0346957.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6324..7409)
                     /gene="mltB"
                     /locus_tag="ACHK8E_004811"
     CDS             complement(6324..7409)
                     /gene="mltB"
                     /locus_tag="ACHK8E_004811"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP0346958.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1767 a         2124 c         1812 g         1860 t
ORIGIN      
        1 accgctacta aagccatatc aagtaagaga tggtgcatcc gggaggattc gaacctccga
       61 ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaactta
      121 ctttactgca gattttttga taccgctact aaagccgtat caagtaagag atggtgcatc
      181 cgggaggatt actcgccttg cggctcgccc ttcgggccgt tgctaaagca acgttatcct
      241 ccctggtgcc tgcggttatt accgccacct cttccatacc ttcatcgctg aaggtacgta
      301 gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc
      361 tatccagctg agctacggat gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag
      421 cttttgaccg catactccct tagcagggga gcgccttcag cctctcggcc acctcaccac
      481 acgcctctta cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac
      541 atattacttt ctgagactta taagtcaaac aatttttccc acacttttat cgtttgcaca
      601 cttcacgctc aattagtctg taaaaacgtc aaaaagagtg ttttatcaac agaaaaacgg
      661 aggtctgtca gatagcagta atgcaaaaaa atggagactt aagttgaatg aacgggagta
      721 aagcgaaaag acaatggagt gaagaagaaa ttttgagggt gcgtctcacc gataaagatg
      781 agacgcggaa agattagtaa ctggactgct gggatttttc agcctggata cgctggtaga
      841 tctcttcacg gtgaacagaa acttccttcg gggcatttac gccaatacgt acctggttgc
      901 cctttacccc taaaactgtc acggtgacct catccccaat catgagggtc tcaccaactc
      961 gacgagtcag aatcagcatt ctttgctcct tgaaagatta aaagagtcgg gtctctctgt
     1021 atcccggcat tatccatcat ataacgccaa aaagtaagca atgacaaaca cattacatct
     1081 aagcagtcat ggcattacat tctgttaaac ctaagtttag ccgatataca caacttcaac
     1141 ctgactttat cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc cacggcattg
     1201 cctgacgctt atattattgc aatttcgcgc tgacccagcc tttcacactg gctaacgctg
     1261 caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct
     1321 tgccgcccac ctgctgagcg accataccaa tcagttcccc tgctttcaca cgatctgtga
     1381 cgtccttaga tacgcctgca atcagagaaa ccttaccttc ggctaccgtt gccagcacga
     1441 taattgtcga ccccagctga ttttttaaat cgtcaaccat ggtacgcaac attttcggct
     1501 caacaccgct aagctcgcta accaacagct taacaccatt aacatcaatt gccttactgg
     1561 aaagatttgc gctctcctgt gcggcagctt gttctttaag ctgttgtaac tctttttcca
     1621 gctgacgcgt acgttccagt actgagcgca ctttatcagc cagattattg ctatcgcctt
     1681 tcagcagatg cgcgacttcg tttaagcgat cgctgtctgc atgaacggtg gcgatagcgc
     1741 cttctccggt taccgcttcg atacgacgaa cgcctgcagc agtacccgat tcagagatga
     1801 tgcggaacag accaatatca ccagtgcgac tggcgtgagt accgccacac agctcggtgg
     1861 agaaatcgcc catgctcagc acacgtacac gttcatcata cttctcgccg aacagcgcca
     1921 tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt ggtttcaatc ggcaagttac
     1981 ggcgaatctg tgcgttcacc aggtcttcga ccgcacgaat ctcttctggt ttcatcgctt
     2041 cgttgtgtga gaagtcgaag cgcagcactt tgtcgttaac cagtgaacct ttctgcgata
     2101 catgagtacc cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca
     2161 gacgaatacg ggcgcgacga gcttcatcaa catcagcctg caccgcgtcg cccactttca
     2221 gagaacccgc agcaagttta ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt
     2281 ccaccgcaaa ggagaagtta gcgcctttca gttcgccttt atcaccaacc tgaccgccgg
     2341 attccgcata gaatggcgtt tgatccagca cgaccacagc ttcctggcct gcattgatgg
     2401 catcaaccgc tttaccatca acaaacagcg cggtcacttt gccgttcagt tccagatggt
     2461 catagccttt aaattcagat gcactgttaa cacggatcat tgcgttgtaa tcggcaccaa
     2521 agccgctggc ttcgcgcgca cgacgacgct gctcttccat tgctgcttca aatccagctt
     2581 cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt caggtcaacc gggaagccat
     2641 aggtgtcgta cagacggaaa gccgtttcac catccagcgt atcaccagaa agttttgcca
     2701 gctcttcgtc cagcagcgcc agaccgcgct ccagagtacg ggcaaactgc tcttcttcag
     2761 tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca
     2821 ttacgtcgat cagcggacca accagtttgt agaagaaggt ttctttcgcg cccagcatat
     2881 tgccgtgacg cactgcgcga cgaatgatac gacgcagtac ataaccacgg ttttcgttgg
     2941 acggcattac gccatccgcg atcaggaacg cacaagaacg aatgtggtca gcgattacgc
     3001 gcagcgattt gttgctcaaa tcggtcgcgc cagtgacttt cgctaccgcc tggatcagcg
     3061 tacggaacag gtcgatgtca tagttagagt taacgtgttg cagcaccgca gcaatacgct
     3121 ccagacccat accggtatct acagacggct tcggcagcgg ttccatcgtg ccatctgcct
     3181 ggcggttgaa ctgcatgaag acgatgttcc agatctcaat gtagcggtcg ccgtcttctt
     3241 ccggacttcc cggagggcca ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc
     3301 acgggccgca cggaccagtg tcacccatct gccagaagtt gtcagatgcg tatggcgcac
     3361 ctttgttatc gccgatgcga ataatacgtt cgcgcgggat cccgacttct ttttcccaga
     3421 tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca cagacgctct ttcggcaggg
     3481 caaaccattt ttcgctggtc agcagttccc atgcaaactg aatggcatcg tgtttgaaat
     3541 agtcgccgaa gctgaagttg cccagcattt cgaagaaggt atggtgacgc gcggtgtaac
     3601 cgacgttttc caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag
     3661 cgcgggaata attacgcttg tcgagcccaa ggaacacatc cttgaactgg ttcatcccgg
     3721 cgttggtaaa caacaaagtt gggtcgttat ggggtaccag ggagctgctg gcaactacct
     3781 gatgtccctt actatggaaa aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc
     3841 tcataattat cctgaaatca agctaacgaa atatcgccac cagctacagc gtgttttaac
     3901 cgccgggctg gtaactgaaa agtgggaata agataagttt tcttgactgg gaagtaaaat
     3961 cccgtatgcg ttcagtcggc aaaatttcgc caaatatcct ggatatcttc catcagatag
     4021 ccacgataga gcagaaaacg ctggatctta actttttctg aaaagacagt tggcaaaggt
     4081 tcgccatatt ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat gtcacattca
     4141 cgcatcgctt tttctgtcgc ttcgcgggaa atacctttct gattcagttc ctgacgaata
     4201 cgcgcaggtc cataaccttt gcggctacgg ctggcgataa agcgcgcaac aaatcggctg
     4261 tcatcgagat agccatgttc atggcaccag gcgataacgc gctcgtaatc ttctgccgtg
     4321 gcatcaatct cttccgggcc atttttgccc atgatcggtg ccgcgagttt acgtcgcaat
     4381 tcttgctcac tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa caggcgagca
     4441 tatgccgggc gacgggacgt tgattctgtc atggcatatc cttacaactt aaaaaagcaa
     4501 aagggccgca gatgcgaccc ttgtgtatca aacaagacga ttaaaaatct tcgttagttt
     4561 ctgctacgcc ttcgctatca tctacagaga aatccggcgt tgagttcggg ttgctcagca
     4621 gcaactcacg tactttcttc tcgatctctt tcgcggtttc cgggttatct ttcagccagg
     4681 cagttgcatt cgctttacct tgaccgatct tctcaccttt gtagctgtac cacgcgcctg
     4741 ctttctcgat cagcttctct ttcacgccca ggtcaaccag ttcgccgtag aagttgatac
     4801 cttcgccgta gaggatctgg aattcagcct gtttaaacgg cgcagcgatt ttgttcttca
     4861 ccactttcac acgggtttcg ctccccacca cgttttcgcc ctctttcacc gcgccgatac
     4921 gacggatgtc gagacgaaca gaggcgtaga atttcagcgc gttaccaccg gtagtggttt
     4981 ccgggttacc gaacattaca ccaattttca tacggatctg gttgatgaag atcagcagcg
     5041 tgttggactg cttcaggtta cccgccagct tacgcatcgc ctggctcatc atacgtgccg
     5101 caaggcccat gtgagagtcg ccgatttcgc cttcgatttc cgctttcggc gtcagtgccg
     5161 ccacggagtc aacgacgata acgtctactg cgccagaacg cgccagggcg tcacagattt
     5221 ccagtgcctg ctcgccggta tccggctggg agcacagcag gttgtcgata tcaacgccca
     5281 gtttacgtgc gtagattggg tccagcgcgt gttcagcatc gataaacgca caggttttac
     5341 cttcgcgctg cgctgcggcg atcacctgca acgtcagcgt ggttttaccg gaagattctg
     5401 gtccgtagat ttcgacgata cggcccatcg gcagaccacc tgccccaagc gcgatatcca
     5461 gtgaaagcga accggtagag atggtttcca catccatgga acggtcttca cccaggcgca
     5521 tgatggagcc tttaccaaat tgtttctcaa tctggcccag tgctgccgcc aacgctttct
     5581 gtttgttttc gtcgatagcc atttttactc ctgtcatgcc gggtaatacc ggatagtcaa
     5641 tatgttctgt tgaagcaatt atactgtatg ctcatacagt atcaagtgtt ttgtagaaat
     5701 tgttgccaca aggtctgcaa tgcatacgca gtagcctgac gacgcaccgc atcacggtca
     5761 ccgctgaaac attcccgccg ggtaatgcct tcaccacggg cagtggcaaa agcaaaccag
     5821 acggtgccga caggtttctc ttcactgccg ccatccggcc cggcgatacc actaatagac
     5881 acggcgtaat cagcacgagc cgctttcagt gcgcctatca ccatttccac cacgacaggt
     5941 tcgctcaccg cgccatgctg tgccagcgtc tcttcgcgta caccgatcat ttgcgctttt
     6001 gcttcgttac tgtaggtgac aaatccgcgt tcaaaccagg cggaactgcc ggcaatatcg
     6061 gtaatcactt tcgctaccca accgccggta caagactcag cagttgttac ggttgcgcca
     6121 cgggctttca gcgactgccc aacctgttcg cttaactgca tcagttcact gtcagtcatc
     6181 acaacctctg tcagtcaaaa atttatgccg gacaagataa cactttcgtc aggaagatgg
     6241 tgttggcgtt gcaattttac gaggaggatt cagaaaaaag ctgattagcc agagggaagc
     6301 tcacgccccc ctcttacaaa tagttactgt acgcgcgcca gcgccacggc ttgtcctaac
     6361 tgccagaccg ccattgcgta atgggtgctg tggttataac gggtaatggt gtagaagttc
     6421 ggcagaccgt accagtactg gtagccggtg ccaacatcca gacgcagcag gctggcttgt
     6481 tgatggttgc ccagcggctg ctgtggcgtt aaacctgccg cggcaagctg cgaaatgctg
     6541 tacttggttt tgaagccatt tggcaagcct ggagcctgac cgtttgccat caccgcaacc
     6601 tgatcacctt tcacccagcc gtgcgctttg aaatagttcg ccacactacc aatggcatca
     6661 accggatccc acaggttgat atgcccgtcg ccgctgaaat ctaccgcata ttgtttgtaa
     6721 gacgacggca taaactgtcc gtagcccatc gccccggcaa aggaaccttt cagattgagc
     6781 ggatcgtcct gctcgtcgcg cgccatcagc aggaaggttt ccagctcgcc agaaaaatac
     6841 tccgcgcggc gtgggtagtt aaatgacagc gttgccagcg catcgaggat gcgagttttc
     6901 cccatcacgc gcccccagcg ggtttcaacg ccgataatcc cgacgataat ttccggcggt
     6961 acaccataca cctgccacgc gcgattcaac gcatcttcat actgattcca gaaaaccaca
     7021 ccgttctgca cgttgtccgg cgtaataaat tttttgcgat aacgcagcca tgcgccgttc
     7081 ggacctgatg gcggtttcac cgatgtggtt ggtgcctggt tatccatcag ccgcagtacc
     7141 gaatccagac gtttcgcctg ggagagaatt tcctgcaact gctgacgatc gaaaccgtgt
     7201 ttgttcacca ttttgtcgat gaactgctgg gcattcggat tattggcgaa gtcaccgccc
     7261 atctgcatca cattgtgctg cggctcaagc aggaagccgc cagacggcgt tccggtggtc
     7321 gtttcagtct cagtaggttt tggcttgctg ctacaggcgg caagcaacac aaaaagggga
     7381 agcaatgtta cataacgacg cttgaacatg aggggtccat ttaacagatt caaccagggg
     7441 caagtatggt aaagcatcac gccccgcaca aggaagcggt agtcactgcc cgatacggac
     7501 tttacataac tcaactcatt ctcctcgcta tccttttatt caaactttca aattaaaata
     7561 ttt
//
LOCUS       DAZGTJ010000156         7410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000156 DAZGTJ010000000
VERSION     DAZGTJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.156"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..675)
                     /locus_tag="ACHK8E_004812"
     CDS             complement(<1..675)
                     /locus_tag="ACHK8E_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0346959.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAV"
     gene            complement(686..1687)
                     /gene="ltaE"
                     /locus_tag="ACHK8E_004813"
     CDS             complement(686..1687)
                     /gene="ltaE"
                     /locus_tag="ACHK8E_004813"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP0346960.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1724..3442)
                     /gene="poxB"
                     /locus_tag="ACHK8E_004814"
     CDS             complement(1724..3442)
                     /gene="poxB"
                     /locus_tag="ACHK8E_004814"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP0346961.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3575..4543)
                     /gene="hcr"
                     /locus_tag="ACHK8E_004815"
     CDS             complement(3575..4543)
                     /gene="hcr"
                     /locus_tag="ACHK8E_004815"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP0346962.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4555..6207)
                     /gene="hcp"
                     /locus_tag="ACHK8E_004816"
                     /gene_synonym="priS"
     CDS             complement(4555..6207)
                     /gene="hcp"
                     /locus_tag="ACHK8E_004816"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP0346963.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6351..7250)
                     /gene="lysO"
                     /locus_tag="ACHK8E_004817"
     CDS             complement(6351..7250)
                     /gene="lysO"
                     /locus_tag="ACHK8E_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP0346964.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1797 a         2020 c         1931 g         1662 t
ORIGIN      
        1 caccgccata aaatgttcaa actgttgctg ataactgagc acttctggtc cggcggcttc
       61 gaagatgcga tgttcgctgg ccggatgatc taacagcgcc accagatagt gcagcaagtt
      121 ttccagcgcg atgggcgtgg tgcgtgaacg aacccagcgt ggcggcgtta acaccggcag
      181 gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcaccaa cgattattcc
      241 ggcacgtagt tcggtcaccg ggacacccgc ttcacgaaga atatccgccg tagcctgacg
      301 ggcgcgcaga tggtcagact gctcatgtgc cggggcctgc aacgaactga ggaagattaa
      361 ttgcttaaca gggacttcgc gtagcgcatc gcggacgttg agagccacct ggcgctcctg
      421 agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagaccg tatcgatatc
      481 ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc aactgacgtt
      541 tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga tctgatgccc
      601 ttgctggctg agagttcgca ccagatgctg accaatgtag ccactggcac cgagaactaa
      661 aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa cgcacgccag tgggcggcaa
      721 cttccgccag ttgttcacgc gagacgtcaa gatgggtcac caggcggaca atcggcgagg
      781 cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca ttttcttccc
      841 cgacgcgaac aaacagcata ttggtgtcct gacgcatcac atccgcgcct gcttcacgca
      901 actgctccgc catccaggcg gcgttatcgt ggtcttcctg caaacgtgcg acgttatttt
      961 tcagggcata catcccggcg gcagccagaa tgccggactg acgcatcccg ccacctgtca
     1021 ttttccgcca gcgaatggca cgtttaatgt aatcacgatt accgacgagt aatgaaccga
     1081 ccggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat tgcgtgatct
     1141 ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg tcaacatgca
     1201 gcgccagatt gcgctcgcga gtaaattccc atgcttcttt caggtattcc cgcggcaaca
     1261 ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa tggatatcgt
     1321 cgggtttgat cttcatcgcc actttatcca gaggtagcgt gccgtcggca gccgcgtcga
     1381 tgggttgcgg ctgaatactg cccagcactg ctgcgccacc ggcttcaaac agatagttat
     1441 gcgcggcctg accgacgata tactcttcgc cgcgttcgca gtgactgagc agagcgacca
     1501 ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa agctctgctg
     1561 catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg
     1621 cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat
     1681 caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt agccagttcg
     1741 ttttcgccag ctcgatcact tcatcgccgc gtccgctgat aattgcgcgc agcatataca
     1801 ggctgaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaac tcttctttgg
     1861 cgaccaccac atccaccaac accggaccgt cgatggagaa ggcgcgttgc agggcttcat
     1921 cgacttcaga ggctttttct acacgaatac ccgtaatgcc gcacgcttcg gcaatgcggg
     1981 caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta gcctccggct ttcatctcca
     2041 tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcaccggc agtttcatct
     2101 gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg cacatggcga
     2161 ccacctgacg ttccggctcg gttgcctgcg cacccagcgc ctgcggcatg gcgttagcca
     2221 tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt agataacgtg
     2281 ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg gcggcaaaat
     2341 gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt ttagctaaat
     2401 cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga aacttgcgat
     2461 cggctttttc ttccaccaat ggcagcaatg cacgcagagt cgacttgata tcgccgacca
     2521 gtgccatatc caccttgctg tgagcgccga tgctggctgg gttgatatca atctgaataa
     2581 ttttggcatc ggtcgggtag aacgcacggt agggaaactg agtaccgagc agcactaacg
     2641 tgtcggcgtt catcatggta tggaaacctg acgaaaagcc gattaatccc gtcattccaa
     2701 catcatatgg attatcgtat tcgacatgtt ctttaccgcg cagggcatgg actataggcg
     2761 ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcacag ccgctgccac
     2821 acatcagggc gatattgctg gaataacgca gcagctgcgc cagtttgcgt aactcttctt
     2881 cttccggtgt tactaccggc tgtggcgcat gataccagtg ggtagttgcc ccttctggcg
     2941 caggttttaa cgccacgtcg cccggtaaca caacaacgga aacgccacgg ttaagcaccg
     3001 ctttgcgcat agcaattgcc agcacttgtg ggatctgctc tgggctggaa accagctcgc
     3061 aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa tagccgctgc
     3121 caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc
     3181 aatcgaacag gccgttgatt aagtgcaggt tgccgggacc gcacgatccg gcacagaccg
     3241 ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa tgccgccact tcttcatgcc
     3301 gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga ccgttcagag
     3361 agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt ttggcgatat
     3421 aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg gtaacaagtt
     3481 tagttcatct gacggagggg gaagggatgg gagaaaaagg aggcactaac ggttaaatag
     3541 cccgatgaaa ggaatatcat cgggcatcag gcgattatgc gagaaccaaa tccccctgcg
     3601 gatggcagga gcaggccagt acataacctt cagcgatttc ggcgtcggtc agcgtcattg
     3661 tgctgctcac cgtatattca ccggaaacca ctttcgtctt acagcagccg caaacacctg
     3721 cgcggcaggc ggcgacaacc ggaacgttat tgctttccag cgcctccagt agcgtggtgc
     3781 caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg
     3841 ccgcttccgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt
     3901 tcacttcctg ctctacccaa tccatatatg gagcagggcc acaggtcatc acggtacgtg
     3961 aggctaaatc agggacacct gccagcagtt cgcgagtgag acgaccagcg ataaagcctt
     4021 cggtaacgtt attttccgcc accagcgtta ccggatagtt acgccactca tcggcgaaaa
     4081 tcacatcctg cggcgtacgc acgttgtaga tcacctgcac atcggcctgt ggacggttct
     4141 tcgccagcca gcgacgcatc gacataatcg gcgtaacgcc gcagcctgcc gccagcaaca
     4201 ggaatttatc ttctgcttta tcatcgcagg taaattcccc catcgcgtcc gaaagccaga
     4261 gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg tcatcaatcc
     4321 gccgcacagt cagggtgatg tattcactca cgcctggcgt ggaggaaatg gtgtaagcac
     4381 gcagcgtttc cgctgagtta cgcacgctga ccagtgcata ttgcccggcg cgatatgggt
     4441 agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat
     4501 gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa ctccttacgc
     4561 gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc cgaatttctc
     4621 gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat
     4681 atttttcaca cccagagaaa gcagcgtcag cagaatgaca atcgctttct gttcaaacca
     4741 ggagagcacc agcgacagcg gcagatcgtt gactccgcag cccagtttct ctgccagcgt
     4801 taccgccaga ataattgctg agtaagcatc gttacattga ccagcatcta ccagacgcgg
     4861 cagaccttcg atatcgccaa actcaagttt attaaagcga tatttaccgc aagcgagggt
     4921 caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaaatagt gacgctcgcc
     4981 gcgtgcgccg tcacagccgc caagcaggaa gatatgacgc agtttttcac ggctcaccag
     5041 atcaatcagc gtatcggctg cgccaagcag tgtctggcga ccaaagccca cggtgatcag
     5101 gtgcggaatt tcgctgtacg ggaagcccgc catctgttgc gcctgggcaa tgactgcaga
     5161 gaaatcttcg ccatccagat gacgcacacc cggccagcca acgatgctgc gggtccagat
     5221 acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca tcacgatggg
     5281 gcctgggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt agttaccgac
     5341 cagatgcttg aatttacgca actccgggta gccatgcgca ggcagcattt cgccgtgggt
     5401 gtaaacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat ctttgagatc
     5461 gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct gagttggcgt
     5521 cgggtgaccg tatttaccgg tttcgcctgc gtccagaatg ctcatcactt tgaagttcat
     5581 ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc aggtccccag
     5641 ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac cgagaacatg
     5701 cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca gacgcaggcc
     5761 gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg ctgcctgacg
     5821 ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg
     5881 ggcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaggg caatcgcttc
     5941 acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa
     6001 ggcacgtggc gcaaagctgt ctacatcgtg gttgataatg ccgtattcac gtgctttcac
     6061 tgcccaggcc gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt cagaggtttc
     6121 tgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg gagtacggat
     6181 agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat ttaatataca
     6241 tgtttaaggt taagacgctt aacgcgggga taaaagagat ttttaatgca actttaaggg
     6301 agattgattt agcgcaattt tggcggcaga gatctaccgc cagagaggta ttatgcagag
     6361 aaaaaggcga tgagaatcgg cactaacaga ctaagaataa aaccgtgaac aatcgccgcc
     6421 gggaccatat ccagcccgcc tgtacgttga agaacgggca gggtgaaatc catcgacgtc
     6481 gcaccgcata agcccagtgc agtagagcgg ctacggcgaa tcagcccagg gattaacata
     6541 atagcaatca gctcacgggc cagatcatta aaaaacgccg cgctcccgat taccggacca
     6601 aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga ggccattgcc
     6661 agcgcggtat tgatgggaag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa
     6721 ctggcaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag
     6781 gtcatgccat tattgcgcaa ctgaataccg acgaggaaaa gtagcagaat taaggtgtat
     6841 tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca
     6901 atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg cgacgggagt
     6961 ttttcttgat gatggtggtt gcgccatggc aggccgcgct ccagccacat cagggcggca
     7021 atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg
     7081 ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag aataaggtaa
     7141 accatccagc ttaatagctg attaataact cttaatgcag ctttttggcg aagcggaatg
     7201 aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaacccag
     7261 tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt
     7321 aaagttgcaa ttaaaaggaa atgttatgca tcagagggag atttttaatt ctatatgagt
     7381 gtttagcatt gttgcgctcc tttcatgtgc
//
LOCUS       DAZGTJ010000157         7318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000157 DAZGTJ010000000
VERSION     DAZGTJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.157"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..1046
                     /locus_tag="ACHK8E_004818"
     CDS             45..1046
                     /locus_tag="ACHK8E_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346965.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNSGKIGKTVDENWVRLIKNMTVLRNDIYTKGDKIEN
                     YTFANVHNNMIYYNAVSKNSKNTEFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEA
                     LNAFIDKGDYLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
     gene            1057..1365
                     /locus_tag="ACHK8E_004819"
     CDS             1057..1365
                     /locus_tag="ACHK8E_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0346966.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(1417..2007)
                     /gene="cobO"
                     /locus_tag="ACHK8E_004820"
     CDS             complement(1417..2007)
                     /gene="cobO"
                     /locus_tag="ACHK8E_004820"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0346967.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(2004..2762)
                     /locus_tag="ACHK8E_004821"
     CDS             complement(2004..2762)
                     /locus_tag="ACHK8E_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP0346968.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2982..4031
                     /gene="sohB"
                     /locus_tag="ACHK8E_004822"
     CDS             2982..4031
                     /gene="sohB"
                     /locus_tag="ACHK8E_004822"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP0346969.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(4067..4318)
                     /locus_tag="ACHK8E_004823"
     CDS             complement(4067..4318)
                     /locus_tag="ACHK8E_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP0346970.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4698..7295
                     /gene="topA"
                     /locus_tag="ACHK8E_004824"
     CDS             4698..7295
                     /gene="topA"
                     /locus_tag="ACHK8E_004824"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP0346971.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1993 a         1654 c         1876 g         1795 t
ORIGIN      
        1 actatttaat tatctgagat aaactcaacg tcaagaggtg ggttatgatt agatatagtg
       61 gaatatttct atcgctgtta atgctttcag gttgtgcaac tgaagcattg aaaaacgaac
      121 aagctgaaac tgttattact gcgggtaaca aagcagtagc ggcgaatgca agtttcatta
      181 aacaatacta tgcttctcgt gaaaacttcc ttgttaattt ctacgcgact aatccggatt
      241 gtgccctgcg acctgattat accattgata taggcggtga tgggaatggc ggaaaattat
      301 gccagcgttc agggccaaat acaaaacaag tacaattagt ccctttcgag aaagaaaaat
      361 tactggcctc aatggaagtc attaatagca tgagcgctta tgtggaactt ctgacaaagg
      421 atatggcaac tacggaaagt cagcaagtgg cattacttaa acaggctctg gatcacgcaa
      481 acaacgcaca aacactactt ggtgtttcac atagcaaaga tttagctcaa tctgcagcag
      541 cggttaaaga tttggtaaat tatttcaatg agttatatat aattaaagtc aattctggaa
      601 aaataggcaa aacggtagat gaaaactggg taagacttat aaaaaacatg actgttttgc
      661 gtaatgatat ttatacaaaa ggcgataaga ttgaaaatta tacctttgcg aatgtacata
      721 ataatatgat ttattataat gcggtatcta aaaactctaa gaatacagag tttaacactc
      781 tgaaaaacag gaaggagttt ctaagttcag taatcaatgt gaatgaaacc agactacagg
      841 catcggcacc tgccatgcct gcgattgagg ctttgaatgc ctttatagat aagggtgatt
      901 atctgaaaag cttatacgtt aataaaacac gcacggcagc tgaacaaaaa agattaaatg
      961 aaatattacg cgcacaggtc attagtgggt taaaggcgtc tgaggggctg gcaaaattac
     1021 ttattgccag cggaataatg ttataggagg ctttttatga acgatgtgca aaaccgtaga
     1081 atactggcat tacttgatat gctgtataat actttgggta ataaaaccac atgtaatgtt
     1141 tgtcataaat attttatcct tatcctcacg gtgatgaaca atctgacgat taactacggt
     1201 actgaaactt cgccagagtt aagcggaatc agttatgaag atgccaatgc gttaattact
     1261 gaactgatat tatttattga agatgttgac gctgagaatc atgaagcata tcagggacat
     1321 ttgaatgaga ttacacgtat tctgaatttc gctattgccg cttgaatata atcatgatta
     1381 aagcacctca agaggcttct tttgaggtgc tttcacttag tagtcaatcc ctatctgcgc
     1441 ttttacaccg gcatcaaacg catgtttcac ggggcgtaat tcactcaccg tatccgccag
     1501 ttcaagaata tcccgatgac agcctcgacc cgtgataatc accgtctgtt gatgtggacg
     1561 ttcatttaac gcctgcacca cttcttccag tggcaaatag tcatacgcca ccatatacgt
     1621 cagttcatcg agcaaaacca tatccagtga aggatcggcc agcattcgtt tggcatgttg
     1681 ccagacttca cggcaggccg cggtatcaga ttcgcgattt tgtgtatccc aggtaaagcc
     1741 cgttgccatc acctgaaact caacgccatg tggctccagt agattgcgct cgccattagg
     1801 ccaggtgcct ttaataaact gcacgatacc cacttttttg ccatggccta ctgcacgagt
     1861 tgccgtacca aatgccgcgg tggttttgcc ttttccattg ccggtaaaga cgatgataat
     1921 accgcgctca tcctgggcct gggccacacg agcatcgact ttttctttca ctcgctgctg
     1981 acgctgttgg tagcgttcat cactcattgg gaaattcctg gtttacggcc cggttgggcg
     2041 tcaaaggtca tgccggtttt acggcggctg tcatcgccca tcagccagag gtagagcggc
     2101 atgatatcag cgggtgtttt aagtttctgt ggatcttcgg tcgggaaggc gctggcacgc
     2161 attgcggtgc gcgtaccgcc tgggttaatg cagttgacgc gcaggcgctg ctgatattca
     2221 tcggccagta cctgcatcat cccttcggtg gcaaatttcg acgctgcata tgcgccccag
     2281 ttggctcgtc cctgacgtcc aacgcttgat gaagtaaaga ccagtgaacc ggcgtccgat
     2341 ttgagtaata aaggaagcag tgcctgggtg agcataaagg tggcattaac gttgacctgc
     2401 atgacgtcct gccagacctg cggattttgt tcgctcattg ggcaaacatc gccgagcaat
     2461 ccggcattat gcaaaacacc atccagacgc ggataattaa cggcaatgcg ctgtgccagt
     2521 tgttggcaat cttcggacgt gcaggtcagc aaatcgagaa taaaccactg tggctgacgt
     2581 ccagtttctt cgtttatgtg gctggctacc tgacgtaatt tttcttcatt acggcccaac
     2641 agaatcactg tcgcaccata gcgtgcatac gtcatcgcgg cttcacggcc aataccatcg
     2701 ctggctcccg tcaccaggat aatgcgatca ttgagtaaat cttgttttgg ctggtaatgc
     2761 atggctactc ctcaacgacg ttgtctgtcg cgctgccagt gtactttatg actttggggc
     2821 tttatgcctg aaacagaagg gcatttcaat cagcctgggg cgataacacc gcaaaatgga
     2881 tactttgtca tactttcgct gcaataacat ctctgcgaga cggcttaaca tgcctgttgt
     2941 aaactgtgag ccaaagcgtt gtttaaccaa ggtggggact cgtggaattg ttgtctgaat
     3001 atggtttgtt tttggcgaaa atcgttaccg ttgtgctagc gattgcggcg attgccgcca
     3061 ttattgtcaa tgttgctcaa cgtaataaac gccagcgtgg cgagttacgg gtcaacaatc
     3121 tcagcgaaca gtataaggag atgaaagaag aactggccgc ggcgctgatg gactcacatc
     3181 agcaaaaaca gtggcacaaa gcgcagaaga aaaagcacaa gcaagaagcg aaagcagcaa
     3241 aagcgaaagc caaactgggc gaggtggcaa ctgacagtaa acctcgcgtc tgggtgctgg
     3301 attttaaagg cagcatggac gcccatgaag tgaactcgct acgtgaagag ataacggcgg
     3361 tactcgcagc attcaaaccg caggatcagg ttgtgctccg tctggaaagc cctggtggca
     3421 tggtgcatgg ttacgggttg gcggcttcgc agctgcagcg tctgcgtgat aaaaacattc
     3481 ctttaactgt aacggtagac aaagtcgctg ccagcggcgg ttacatgatg gcctgtgtgg
     3541 cggacaaaat tgtttccgca ccgtttgcta ttgtgggttc cattggcgtg gtggcgcaaa
     3601 tgcccaactt taaccgcttc ctgaaaagca aagatattga tatcgaactg cacaccgccg
     3661 ggcagtataa gcgcacgctg accttgctgg gtgaaaacac cgaagaaggg cgggagaaat
     3721 tccgcgaaga gctgaacgaa acgcatcagt tatttaaaga ttttgtgaag cgtatgcgtc
     3781 cgtctctgga tattgaacag gtggcaacgg gtgaacactg gtacggacaa caggcggtag
     3841 agaaaggcct ggttgatgaa atcaacacca gtgatgaagt tattcttagc ctgatggaag
     3901 gccgtgaagt ggtaaatgta cgctatatgc agcgtaaacg actcattgac cgattcaccg
     3961 gcagcgcggc agagagcgcc gatcgattgt tgctacgctg gtggcagcgg ggtcaaaagc
     4021 cattgatgta aaagacaaac gcgagggtaa gacctcgcgt tttgctttaa tcaaccaggt
     4081 gatatttttc tgaaagcaca tgggccaggt gtttgaacat attaaacacc gcggtgcttt
     4141 tggctgttgg caatccttgt tcatctaaaa agtagtcgcc ggtaaatacc agcacgccat
     4201 tacgctgcgt gacgccggtg gcttcaatcc ctgcgagcgt atcctcatgc tcacgaatga
     4261 ttttgttggc ctctttcaac agcgtctcgc ggtcgatggg ttgtgtctct ttgttcatta
     4321 tttactcctt aaacaaggac attagtctac gccaggcatg gcttgcagac aaatatacca
     4381 cgctggtggc aagagcgcct tactggcaac tttggatttt gcatgctaat aaagttgcgt
     4441 atcggatttt atcaggtaca gtgtgacgct ttcgtcaatc tggcaataga tttgcttgac
     4501 attcgaccaa aattccgtcg tgctatagcg cctgtaggcc aagacctgtt aactcagtca
     4561 cctgaatttt cgtgaacaga gtcacgacaa ggggttgata tccgcagaga gcgagtccat
     4621 atcggtaact cgttgccagt ggaaggttta tcaacgtgcg acgcattcct ggaagaatca
     4681 aattaggtaa ggtgaatatg ggtaaagctc ttgtcatcgt tgagtccccg gcaaaagcca
     4741 aaacgatcaa caagtatctg ggtagtgact acgtggtgaa atccagcgtc ggtcacatcc
     4801 gcgatttgcc gaccagtggc tcagctgcca aaaagagtgc cgactctacc tccaccaaga
     4861 cggctaaaaa gcctaaaaag gatgaacgtg gcgctctcgt caaccgtatg ggggttgacc
     4921 cgtggcacaa ttgggaggcg cactatgaag tgttgcctgg taaagagaag gtcgtctctg
     4981 aactgaaaca actggctgaa aaagccgacc acatctatct cgcaaccgac cttgaccgcg
     5041 aaggggaagc cattgcatgg cacctgcggg aagtgattgg tggtgatgat gcgcgctata
     5101 gccgagtggt gtttaacgaa attactaaaa acgcgatccg ccaggcattt aacaaaccgg
     5161 gtgagctgaa tattgatcgt gttaatgccc agcaggcgcg tcgctttatg gaccgcgtgg
     5221 tggggtatat ggtttcgccg cttctatgga aaaagatcgc tcgtggtctg tctgccggtc
     5281 gtgtgcagtc ggtggcggtt cgcctggtgg tcgagcgtga gcgtgaaatt aaagcgttcg
     5341 taccggaaga gttctgggaa gtcgatgcca gcacgaccac gccatctggt gaagcgttgg
     5401 cgttgcaggt gactcatcag aacgacaaac cgttccgtcc ggtcaacaaa gaacaaactc
     5461 aggctgcggt aagtctgctg gaaaaagcgc gctacagcgt gctggaacgt gaagacaaac
     5521 cgacaaccag taaacctggc gctcctttta ttacctctac gctgcaacaa gctgccagca
     5581 cccgtcttgg atttggcgtg aaaaaaacca tgatgatggc gcagcgtttg tatgaagcag
     5641 gctatatcac ttacatgcgt accgactcca ctaacctgag tcaggacgcg gtaaatatgg
     5701 ttcgcggtta tatcagcgat aattttggta agaaatatct gccggaaagt ccgaatcagt
     5761 acgccagcaa agaaaactca caggaagcgc acgaagcgat tcgtccttct gacgtcaatg
     5821 tgatggcgga atcgttgaag gatatggaag cagatgcgca aaaattgtac cagttaatct
     5881 ggcgtcagtt cgttgcctgc cagatgaccc cagcgaaata tgactccacg acgctgaccg
     5941 ttggtgcggg cgatttccgc ctgaaagcac gcggtcgtat tttgcgcttt gatggctgga
     6001 caaaagtgat gcctgcactg cgtaaaggcg atgaagatcg tatcttaccg gcagtcgata
     6061 aaggcgatgc tctgacgctc gttgaactga caccagccca gcactttacc aaaccgccag
     6121 cccgtttcag tgaagcatcg ctggttaaag aactggaaaa acgcggtatc ggtcgtccgt
     6181 ctacctatgc gtcgatcatt tcgaccattc aggatcgtgg ctatgtgcgt gtagaaaacc
     6241 gtcgtttcta tgcggaaaaa atgggcgaaa tcgtcaccga tcgccttgaa gaaaatttcc
     6301 gcgagttaat gaactacgac tttaccgcgc agatggaaaa cagccttgac caggtggcaa
     6361 atcacgaagc agagtggaaa gctgtactgg atcacttctt ctcggatttc acccagcagt
     6421 tagataaagc tgaaaaagat ccggaagagg gggggatgcg cccgaaccag atggttctga
     6481 ccagcatcga ctgcccgacc tgtggtcgca aaatggggat tcgcacagcg agcaccgggg
     6541 tattccttgg ctgttccggc tacgctctgc caccgaaaga gcgttgcaaa accaccatca
     6601 acctggtgcc ggaaaacgaa gtgctgaacg tgctggaagg cgaagatgct gaaaccaacg
     6661 cgctgcgcgc aaaacgtcgt tgcccgaaat gcggcacggc gatggacagc tatctcatcg
     6721 atccgaaacg taagttgcat gtctgtggta ataacccaac ctgcgacggt tacgagatcg
     6781 aagagggcga attccgcatt aaaggttatg acggcccgat cgttgagtgt gaaaaatgtg
     6841 gctctgaaat gcacctgaaa atggggcgtt tcggtaaata catggcctgc actaacgaag
     6901 agtgtaaaaa cacgcgtaag attttacgta acggcgaagt ggcaccaccg aaagaagatc
     6961 cggtgccatt accggagctg ccgtgcgaaa aatcagatgc ttatttcgtg ctgcgtgacg
     7021 gtgctgccgg tgtgttcctg gcggccaata ccttcccgaa atcgcgtgaa acacgtgcgc
     7081 cactggtgga agagctttat cgcttccgcg accgtctgcc ggaaaaactg cgttatctgg
     7141 ccgatgcgcc acagcaggat ccggaaggta ataagaccat ggttcgcttt agccgtaaaa
     7201 ccaaacagca atatgtctct tcggaaaaag acggaaaggc gactggctgg tcagcatttt
     7261 atgttgatgg caaatgggtt gaagggaaaa aataaccttt aattctgtca ggttttta
//
LOCUS       DAZGTJ010000158         7315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000158 DAZGTJ010000000
VERSION     DAZGTJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.158"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..872
                     /locus_tag="ACHK8E_004825"
     CDS             <1..872
                     /locus_tag="ACHK8E_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0346972.1"
                     /translation="GYIPNRTAGALASGHSDTVAVLVPSLTDKTSSRFMQSLQQVLNK
                     NEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVV
                     GSPFSPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAML
                     SYYQNSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLL
                     KIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRI
                     VQFNYQIELRQST"
     gene            1075..1755
                     /locus_tag="ACHK8E_004826"
                     /pseudo
     CDS             1075..1755
                     /locus_tag="ACHK8E_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1772..2080
                     /locus_tag="ACHK8E_004827"
                     /pseudo
     CDS             1772..2080
                     /locus_tag="ACHK8E_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2183..3289)
                     /locus_tag="ACHK8E_004828"
                     /pseudo
     CDS             complement(2183..3289)
                     /locus_tag="ACHK8E_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4056..6104
                     /gene="ireA"
                     /locus_tag="ACHK8E_004829"
     CDS             4056..6104
                     /gene="ireA"
                     /locus_tag="ACHK8E_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFP0346973.1"
                     /translation="MKNKYIIAPGIAVICSTVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
     gene            6778..>7315
                     /locus_tag="ACHK8E_004830"
     CDS             6778..>7315
                     /locus_tag="ACHK8E_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0346974.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDESSQGYDVHG
                     DNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAI
                     AIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSSEAS
                     GDGSIVVGSRSTAAGDLSV"
BASE COUNT         2096 a         1568 c         1668 g         1983 t
ORIGIN      
        1 tgggttatat tcccaacaga actgctggcg cccttgcatc tggtcacagt gatacagtcg
       61 ccgtactggt tccttccctg acagataaaa ccagctcccg ctttatgcag tcgttgcagc
      121 aggtgcttaa taaaaatgaa ttccagctac tgcttgggtg tcatgagtat aatcagctca
      181 aagaagctga aatcctgatg acgcttcttc aaggtaaccc ggcggcgtta gttatttttg
      241 gttctcagtt ggctgataaa actcaccaga tactcgagaa aacaaatata cctaccatta
      301 acgttgtcgg ttcccctttc agtccggctc aaattaccat cgaaacagca ttttttgaag
      361 catcacataa actgaccgaa catctgctgg aacagggtta taagaatatc ggatttatcg
      421 gtgcgcatat ggacaaccgc ctgcaacgtc agcaactgaa cggctggcat aaagcaatgc
      481 tgagctatta ccagaactct gatcttgtta tcaccatgcc gggtgccgca agtttgcaat
      541 tggggcgtta cgcattaaac gagatattac tgcgacaacc agagctggat gctgtgattt
      601 gcagccacga agagattgcg ctgggtatca tgtttgagtg ccagcggcgt ttgttaaaaa
      661 tccctggaaa tatcgctgta gcttgtcttg acggttcaga cagttgcgat caaacacacc
      721 caaccttgac atccatccgt attgactaca aaaaaatggg gacagagacg ggtaagctgc
      781 tgattggtct attgaataat aatcatgatg aatcagaaga gtcgcgtatc gtgcaattca
      841 actaccagat agaattacgt cagagtacat gagctcaaaa ggggattatt gcaaagttag
      901 ctattgtggt agggatagcc gtttccggct attccccgcg cagagccgga tgtgtgctgc
      961 taacgcatcc tgtcaacgac ggatgaaaag ttatccactt atatctccac caacggccca
     1021 atattgtatc gttcgtgaca catccatttc tacttcaggt atttggcgta gtagcgggtg
     1081 ctaaaaattt gccgcaaaac cgcaatatca gcccgcagag gcgctctcca gattatccat
     1141 tcctatatta tctctgcacc ggatgaatta ctcgaacaac tcagaaatat gacacatatg
     1201 caacttatca ggactctggg atcctggagg cctgatgcca gtgaatacag taatgttacc
     1261 aacgtttatc gcattacatt gaaatccctt gttcgacgct atttcgagtt tcatgatgaa
     1321 atcaccaact tggatgtcat gattgcggca attgttgatg aactggcacc tgaactgatt
     1381 aaacgtaatg ctgttggata cgaaagcgct ttgcagttgt tgatctctgc cggagacaat
     1441 ccctaacggt taagaaccga atcaggtttt gcggcgttgc gcggtgtcag ccagtttccc
     1501 ctatcttctg gaaaaacgaa ccattaccga cttaatcggg gtggggagcg tgttgcaaat
     1561 agtgcacttt acatcatcac tataggatgt ttacaaactg acgataaaac aaaggaatat
     1621 gtctccaggc gagtagccga aggacataca aaaatggaag caatacgctg tctgaagcgc
     1681 tatatctcac gcgaagttta tacattacta cgaaatcaaa acaggcatgt taactgcatc
     1741 ccgataacgt cttgactctt agaagggcgt ccaaggcagt cattattcga gcaggcagtt
     1801 ccggcagtta ttgtgtcgat accagatcag gcagagtatg agccggcgcg gaaaatgctg
     1861 ggataacagc tcaatggaac gcttcttcag gagtctgaag aacgaatgga tgccggtggt
     1921 gggttacgta agcttcagcg aggcagctca cgccataacg gacgatatcg ttggatatta
     1981 cagcgcacta agaccgcacg aatataacag tgggttatcc ccaaacgaat cggaaaatcg
     2041 atactggaaa aactctaact cggtggccag tttttgttga ccacttcagc catcgccaga
     2101 cgctaacatg agttactctt gttctgggct acgtcacctt cagggaactg gttatcctca
     2161 ttctttattt gtgacattgt ggttattgtg cgacaaacat ggctatcccg ccccatatcc
     2221 cagataaaac cacctgctca cgtgcgacag cgacaattgt aatattgaca ttctttcctt
     2281 tggcctgaag ttttcgagac ctcttgcaaa gcctgagttg agcatcccat gctctgtcaa
     2341 tgacggggcg gggtaaattt tcctgatttt ttgtattgcc ggactgatgc gtgtcggatg
     2401 ccggtaactc cacgctgcct caaccaatag ctttctggca tagctgtttc cggctttggt
     2461 aatgctgccc tgatgtctgc ttccacccga tgaatattct gatggcgtga taccaaacca
     2521 tgacatcagt tgtcgtggat gttcaaaacg ggtcagatca cccagttcag aaattaatcc
     2581 aaccgcagta ataaactgaa tccctcgcat ggcttgctgc gcctctacta ccggatacag
     2641 cctccattcg gtaacggcct ccttcgggcg gattccagcc gttcacattg tgcctgccgg
     2701 tcttctattg tacaacggtg ttcgtcaaaa gctaattgtc gccagggact ttcgaatgag
     2761 tatttgctga gccagtgtcg gtgtgcaggg ccccaatctg ctcgcccgac ataatggacc
     2821 ccatgaacca gaagaaacga tttcagacgt tgccttgcat gccgtaaatc atcgcgggca
     2881 gatgcccatg cccgggccaa atcccggaat gcctcatctt caacgccgga tacgtagaca
     2941 gcagaaagat ctcctgcacg cagtgaacgc acaagtctga tggcatcacg gtgtcggttt
     3001 taccccgctc ccccggtttt ttcgggataa gtgagggggc gcagaccatg cagtcaaaac
     3061 cggatttcac cagccgacga taaagtccat aaccacaggg tcccgcttta taaacgatac
     3121 tgacctgcga tgactttgac ctgatacgtt gataaagatt atgaatgtca gtaggtgatg
     3181 tgctaatttt acccatcagt tcaacaggtt ctgaattgat agcataggag acagtaatcg
     3241 attctttgtg aacatctaat tcgacgtaca acatgttatt gttgttcatg ctggcctccg
     3301 tggtggaaat tccagggggg tagcaacagc tcacccatgc ggctctgaca acgatgttaa
     3361 cccgcattaa atatcccacg ttggacagca tctgtctcac ggaaagtcat actgccttac
     3421 gtgcatattt caccttacgc cttcactgtc tgtcatcgga agtactgatt tatattgggg
     3481 ctttgcactt actgtcattc acgggaaaag atggtggtga acagtacgat actgtcttgg
     3541 ctgttgccga gggaagattc aggagtggta agggggttag tggtgtaaga tgagtctgga
     3601 gctctgactg gtcagtgacg cgaaactcaa tctgccagga taaactcccc cttgcttttg
     3661 cgcagaagca gtgggccttg gttcgcttat gtcctgcatc tacaagcgaa tgactgaccg
     3721 gagactccca tatctggtca gtcgcccatt tttattttcc accacatcgg acaagcatac
     3781 cgtaataaac agcaccagtc tcctgttcac tcttgcttga tttactggaa tgctgtttat
     3841 ggagatatcc ggttgaaaga ttttacctct tcgacaggac tataaattat acagtaagga
     3901 gtccctcgta gaaaggggct gattctcatc aagtcggaca ggttcaaagt gttacctgaa
     3961 tgagctaaat gttatttacc ttcctttctg ttcattgtaa attttttctt gctaacgcaa
     4021 atcattatca ttacgatttt agaaaaggga aatttatgaa gaacaaatat atcattgctc
     4081 cgggcattgc cgtgatatgt tctacagtca tatcatcagg ttatgccagt tctgataaaa
     4141 aagaagatac gcttgttgtt actgcctccg gcttcactca gcaactcaga aatgcaccgg
     4201 ccagtgtttc agtcattact tcagagcaac tgcaaaaaaa accggtttca gacctggtcg
     4261 atgcggtaaa agatgttgaa gggattagta tcaccggtgg gaatgaaaaa ccggatatca
     4321 gtatacgtgg gttaagtggc gattatacat tgattctggt cgatggacga cgtcagagtg
     4381 gtcgggaatc cagaccaaac ggcagcggcg gttttgaagc cggatttatc cctcctgtgg
     4441 aagcaattga gcgtattgaa gttatacgtg gccctatgtc ttccctgtat ggttccgatg
     4501 ctatcggagg agttattaat atcataacca aacctgttaa taaccagaca tgggatggcg
     4561 tacttgggct tgggggaatt attcaggaac atgggaaatt tggtaactca accacgaatg
     4621 acttctacct gtcaggccca ttggttaagg ataaacttgg tcttcagtta tatggaggaa
     4681 tgaactatcg caaggaagat agtatctctc agggaacacc agcaaaagat aataagaata
     4741 taacggcaac actccagttt actccgactg aaagccagaa gtttgttttt gaatacggaa
     4801 aaaataacca ggaacataca ttaacacctg gtgattctct tgatgcctgg actatgcggg
     4861 gaaatcttaa acaaccaaac agtaaaagag aaacacataa ctcacgtagt cactgggtag
     4921 cagcatggaa tgcccagggc gaaatactgc atcctgaaat tgctgtttat caggagaaag
     4981 ttattcgtga ggttaaatct ggtaaaaaag ataaatataa tcattgggat cttaattacg
     5041 agtcaagaaa accggaaata accaacacga tcatagatgc aaaagtgacg gcatttctgc
     5101 cggaaaatgt gctgaccatc ggaggtcaat ttcagcatgc agaactccgt gatgactcag
     5161 ccacaggtaa aaaaacgaca gaaacacagt ctgtttcagt taaacagaaa gctgttttta
     5221 tagaaaatga atatgctgca acggattctc tcgccctgac tggagggctg cgcctcgata
     5281 accatgaaat ctatggcagt tactggaatc caagattgta tgctgtttat aacctgaccg
     5341 ataatctcac tctcaaagga gggatcgcaa aagcatttcg ggccccttcg attcgtgagg
     5401 tcagtcctgg atttggaaca ctgacgcagg gtggtgcctc tattatgtat ggaaacaggg
     5461 acctgaaacc ggagaccagt gtaaccgaag agatcggtat tatttataat aatgacagtg
     5521 gtttttcagc aagcgcgact ctgtttaata cagattttaa aaataagctg accagttacg
     5581 atataggcac aaaagatcca gtcactgggc taaacacttt tatttatgat aatgtgggtg
     5641 aggcaaatat cagaggtgtg gagcttgcaa ctcagattcc tgtgtatgat aaatggcgtg
     5701 tatctgcaaa ctatacattc actgactccc gtcgagaaag cgatgacgaa agtctcaatg
     5761 gcaagtcgct gaaaggggaa cctctggaaa gaactcccag acatgcagcc aatgcaaaac
     5821 tggaatggga ttacactcag gatattacat tttattcatc tctgaattat acaggaaaac
     5881 aaatctgggc agcacaaaga aatggtgcca aggttccccg tgttcgtaat ggattcactt
     5941 ctatggatat tggtatgaat taccagttcc tgccaggcac gctgattaat tttgccgtac
     6001 ttaacgtcac agacagaaag agcgaagata tcgataccat tgatggtaac tggcaggtcg
     6061 atgaaggacg tcgttactgg gctaatgtaa gaatatcctt ctgagagaat atagataacc
     6121 tgaggccgtg cgcctcaggt accagtatag taatcagtaa cgtcagcttc caggttgtaa
     6181 tgcttttaat tttgataaaa ataaacttaa taaattacat ttgttaaata ttgataattt
     6241 taatttggtt ttattactcc agttatactc acactctatc tttacacaat tcatcaccat
     6301 tgcacctcca tagcctgttg atttaaaaga tttaatttcc tgtgaaacaa tcaggcctgt
     6361 atgaacctga ttataccaca taatctttta agttatttaa atttaactct cgggcttttc
     6421 tgtcagtaaa atcagaaaaa ttgcaggatt tcgttttttt gctgcgaagt gaaatgtatc
     6481 tcatttttac tgccttcgcc agtgcgtcag gcaatggcag gtagtgtacc tgcattctgc
     6541 tgtaacgcgt aatgtcgggt agtttactta aacttaatat gttttatgtt cgcattatta
     6601 taaatcactt tattgccttt tgtctgcttt ttaatggttc ccgttttgtt cacttctggc
     6661 tttataaaaa atggatgctg aagaaatgaa ggcagtccgc cttacataat attcatgcat
     6721 gatttttatg tcattccgac tgagtggaat gaaaataaag gagaaaagag ggtatatatg
     6781 aacctcagaa aaaaacatat atgcatagcc atatcacttg ttcttaatcc gttccctgca
     6841 tcactggcag cacaaaacac taatgtagtt gaagttgatg aaagcagtca ggggtatgat
     6901 gtgcatggag ataactggaa gagcattgaa atagggacaa cgcaggaagt taaagcatcc
     6961 cccgataaaa atattaattt cgatcataat tctcctgttg cagaaggacg taactctatc
     7021 gcgattggta ccagtgcaga ggctggtaaa gaaatgacgg ataaaatggc aggggaagcc
     7081 atagctattg gtgccaaggc gaaagcaact aaagaacagt caattgcaat aggtggcgat
     7141 accaaagcta ccggatgggg ggctattgtt attggtggcg atgatttaac ccctttgcat
     7201 ggtgacagat tcggtgataa agaaattccg aataattact cgtcatctga agcatcagga
     7261 gacggaagta ttgttgtggg ctcaaggagc acagctgcag gagacctgtc tgtgt
//
LOCUS       DAZGTJ010000159         7299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000159 DAZGTJ010000000
VERSION     DAZGTJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.159"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            155..1036
                     /gene="htpX"
                     /locus_tag="ACHK8E_004831"
     CDS             155..1036
                     /gene="htpX"
                     /locus_tag="ACHK8E_004831"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP0346975.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(1082..2455)
                     /locus_tag="ACHK8E_004832"
     CDS             complement(1082..2455)
                     /locus_tag="ACHK8E_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0346976.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            2632..3423
                     /gene="kdgR"
                     /locus_tag="ACHK8E_004833"
     CDS             2632..3423
                     /gene="kdgR"
                     /locus_tag="ACHK8E_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP0346977.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(3567..3806)
                     /locus_tag="ACHK8E_004834"
     CDS             complement(3567..3806)
                     /locus_tag="ACHK8E_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP0346978.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            3965..4108
                     /gene="mgrB"
                     /locus_tag="ACHK8E_004835"
     CDS             3965..4108
                     /gene="mgrB"
                     /locus_tag="ACHK8E_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP0346979.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            4183..4470
                     /locus_tag="ACHK8E_004836"
     CDS             4183..4470
                     /locus_tag="ACHK8E_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP0346980.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            5140..5283
                     /locus_tag="ACHK8E_004837"
     CDS             5140..5283
                     /locus_tag="ACHK8E_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP0346981.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            5296..5505
                     /gene="cspE"
                     /locus_tag="ACHK8E_004838"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             5296..5505
                     /gene="cspE"
                     /locus_tag="ACHK8E_004838"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0346982.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            5671..6480
                     /gene="rlmA"
                     /locus_tag="ACHK8E_004839"
     CDS             5671..6480
                     /gene="rlmA"
                     /locus_tag="ACHK8E_004839"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP0346983.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(6477..7043)
                     /gene="mntP"
                     /locus_tag="ACHK8E_004840"
     CDS             complement(6477..7043)
                     /gene="mntP"
                     /locus_tag="ACHK8E_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP0346984.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDKEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(7175..7265)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT         1976 a         1642 c         1826 g         1855 t
ORIGIN      
        1 acagcagcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga
       61 cttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata
      121 ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct
      181 aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc
      241 gagcagcgtt caggggctga tgatcatggc cttgctgttc ggttttggtg gttccttcgt
      301 ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca
      361 accgcgtaac gaaagggaac gttggctggt caatacggta gcaacccagg ctcgtcaggc
      421 ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaatg cttttgcaac
      481 cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag
      541 cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat
      601 ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat
      661 tctggcgcag cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa
      721 cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct
      781 ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccacgccg atgcgggttc
      841 agcaaaactg gttggtcgcg agaaaatgat tgctgcattg cagcgcctga aaaccagcta
      901 tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacgggaagt cgaaatcgct
      961 cagtgagttg ttcatgactc acccgccact ggataaacgt attgaagctc tgcgtacggg
     1021 tgaatacctg aagtaatatg aagtaaatac taaaaagcgc gtcctgatcg acgcgctttt
     1081 tttatgccct ggatcgtggc tgagtgatac gtaaaccact gacacaggct gcaatcaccg
     1141 ccagaatagc cgcagctatc agcgagacgt gcgtaccatt atcaccaaac tgatttagca
     1201 tcagcgccac cagagccgcg ccgctactct gacccagaag acgagccgtc cctaacatgc
     1261 cactggctcc accgctacgt tcgcgaggag cggaggtaat aatggtgtgg ttattgggtg
     1321 actggaacaa gccaaatcca gcaccacata agatcatcgg ccagataata ttgatatccg
     1381 caggtgacgc tggcagcaga actagggaaa aaagccccgc agccatgatg aataacccta
     1441 aagcacccag caatcctgca tgtacgcgtt caatcaaata gcctgccagt ggagccatca
     1501 ccattgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga
     1561 gcacggtttg caggtaaaag ggaagggaaa ccattgccag catttgtgcg cagaaagagc
     1621 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca
     1681 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacgaca accattacca
     1741 ccagttccgc accaatcaat gtcagcgatt gcccctgagc gaaaccactc aatgcagtaa
     1801 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
     1861 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccca
     1921 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
     1981 ttgtcggccc ggcagcagaa gaaacggcaa caataaacga gtttatgccc atccctctac
     2041 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
     2101 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
     2161 cgcagaatag tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc
     2221 caaacatatc gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg
     2281 cgttcactac ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca
     2341 aggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta
     2401 atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa
     2461 ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg
     2521 atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt
     2581 ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac
     2641 gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg
     2701 caggcgctgg gtgaagagcg cgaaataggg ataaccgagt tgtcgcagcg cgtcatgatg
     2761 tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag
     2821 gaaggggagt cggagaaata ttcgctgacc ctgaaattgt ttgaactggg cgctcgcgcg
     2881 ttacaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat ctcccgcctg
     2941 accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa
     3001 attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac
     3061 agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gaagcaaatt
     3121 cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta
     3181 ttacccgttc tggaccaggt gcgcgagcag gggtatggcg aagataacga agagcaggaa
     3241 gaagggctgc gatgcattgc ggttccggta tttgatcgct ttggcgtggt cattgccggt
     3301 ttgagcatct ccttcccgac gttacgtttc tctgaagagc gtttacagga atatgtcgca
     3361 atgttgcata ccgcggcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc
     3421 tgatgagagt aagaacctgt ctgaatatca aacagacagg ttctttattt agcatgagaa
     3481 aaataaagtt gaaggtggcg ttatattaaa cgcacttgcg ataagaatat tttactcatg
     3541 agtgagaatc tggttattta ttacccttaa ccattatcga ccacaatatt gcttttacgt
     3601 aacaacgggc aatctgttat ccccagaaaa ccacttttag tgtgcaggta ttgtaccgtg
     3661 ctggtgcctc tggcggtcag ataggtacat tgcaaaccta atcccgcggc attctctttg
     3721 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
     3781 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
     3841 tagcggcaat atgcgctgaa gcaagcgact cattctgaaa aagcacgaat atcgacatag
     3901 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aaacggagat
     3961 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
     4021 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
     4081 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
     4141 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
     4201 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttctttt tatcgggctt
     4261 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
     4321 gaggcgttgc tggatcagca aaaacgtcaa aatgctctgt tacgtcgttt gtgcgaagca
     4381 aacgaaccag agaaagcaga taaaaagacc gttgagagtc aaaaatcggt tgaagacgaa
     4441 gatattattc gtctggtcgc cgaacgataa tctttcctca atcttgagta accccgtcgt
     4501 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcgtcaaatt
     4561 aaaactctgg tctgacttca aataattatc atattattta ttttcgtaac gggatatcag
     4621 aaaaagatat ttagcccatt aatttattag gatgtttaca tcggatttgt gattaagcgt
     4681 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgcgcattca tcagtttttc
     4741 ttattttaaa tttacaatac cattggggat tgacttctct ttggggtaat tggtggccgt
     4801 taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcagaaaat
     4861 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
     4921 gaactatgtt agaaaatacg tcagtttaag tatctgcctg aactggcaag gttaagcaca
     4981 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
     5041 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
     5101 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
     5161 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
     5221 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
     5281 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
     5341 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctctgct
     5401 atccagggta atggtttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
     5461 gacggtcaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
     5521 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
     5581 ttgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
     5641 tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact ttgccatcag
     5701 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
     5761 aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac
     5821 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
     5881 cgtgatgcaa ttgtcgccca actgcgggaa cggcttgatg aaaaggccac agcggtgctg
     5941 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgct gcccgaaatc
     6001 accacgtttg gcctggatgt ctcgaaggta gcgataaaag cggcggcgaa acgctatccg
     6061 caggtcactt tttgtgttgc ttccagccac cgtttgccgt tttccgatac tagtatggac
     6121 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcgcgagt agtgaagcct
     6181 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
     6241 atttacaatg aagtacatct tcatgcacca catgcagaac aactggaagg ttttacatta
     6301 cagcagagtg atgagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
     6361 ttgcagatga cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca
     6421 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
     6481 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagcaccag cccgccgaga
     6541 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtc
     6601 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
     6661 aaggcgagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
     6721 agtagccaga aaccgtgccg acggcgcggc tctttatctt catcatctgc gccacgaaaa
     6781 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtgattc
     6841 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggtgtcagg
     6901 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
     6961 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
     7021 agaacagtag cagtgatatt catgacaacg tcctgaccgg ggtatccata aaacacatca
     7081 tcgcccccag caaacatgca tacatgaaca acaagccgcg atgggttttc gcgttcatgc
     7141 aggcatagcg attgatgtgc atatggtctc gcctgactga aagtgttcag tccgtacgtg
     7201 ccacgttttg caacgagtat gttgacacgt acatttccgg aatctggaaa tcggctactc
     7261 cccaaatgag ggcgcaacct taacatattt tgagaatat
//
LOCUS       DAZGTJ010000160         7150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000160 DAZGTJ010000000
VERSION     DAZGTJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.160"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(171..2954)
                     /locus_tag="ACHK8E_004841"
     CDS             complement(171..2954)
                     /locus_tag="ACHK8E_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0346985.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3011..5383)
                     /locus_tag="ACHK8E_004842"
     CDS             complement(3011..5383)
                     /locus_tag="ACHK8E_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP0346986.1"
                     /translation="MKRVLIPGVILCGADIAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5421..7106)
                     /locus_tag="ACHK8E_004843"
     CDS             complement(5421..7106)
                     /locus_tag="ACHK8E_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP0346987.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1862 a         1629 c         1594 g         2065 t
ORIGIN      
        1 ttgttatttt tatgcacttt ctacgcctcc tgaattatca cgtaaaaatc agaccttaaa
       61 atatcactat tagtacttgg ttattatttt gaacgcattt ataaaattat tacataaaaa
      121 tagcgaatat tgataaaatc cccgccaacg atgtgttgac ggggctgtta ttattttggc
      181 aataatactc cggtataggt attcaccgga tgagaaagat attgtttaac ggcagtgtta
      241 acattctcta ccgtcatttg tttcaataat tgctcctgct cagtccatgc tgcaggatcg
      301 tcatattgaa taagactatt tacaatggtg ttcgctaatt gttgaacgct acgctgttgg
      361 atatcgaggc tgcgctgaac gttttgctgg tattcgttca gttcttgctc actgatccct
      421 ttagccagac gcttaaccat cacttcattc gctaacgtta acagttcatc atgtcgttcc
      481 ggttgacaag taaaagccag caaatgactg atatctttgg cctgaggatc aaccgagagg
      541 cgagaagaaa cgctgtatgc cccagaggcc tgttcacgaa tattaacgcg tagatctttt
      601 gccagtgcga cgttaaaagc atcgagcgcc atacgcgtct ccagattaac cggtgtccgg
      661 gaatcataac gcttccactg tgaaacctgt gccacaggtt cattttgttc ttttacagta
      721 accgatgcgt tgtccgtcgc gcgagttaat ggtttacctg cggctaatgg cgaatcagag
      781 tgtttgattg atcctaagta aagcgtaatt aacggcagga gtttgtcttc tgcgacatta
      841 ccgacaatga caaacgtgat atccgctgga gatgaaaaca actggcgatc ggcagccagc
      901 gcatctgcgg cagtaaactg tgcaatctga ttttcttgca gtaatttcgt gcggtcatca
      961 gcatagcgcg tctcatacat ctgctgggcg aatttctccg ctggacgctg gtcgagcgtt
     1021 ttcaacgcct gaatttgagc attttgtagc gatgcccaaa tattatcgtt aatcgtgctg
     1081 tgggtgattc gctggttaat caactggaaa ccaggttcag ggttattagt ccgcgcgcta
     1141 acagagagca atgtattcat gccactgact ttactgctca tggttaccga gttttccgca
     1201 ctccagcgtt tcaggctgga agaggagagt tcaccaacgc cgcttccgct tactgcttta
     1261 tttgccagcg cgataagtga tttttgctgc gcagggaaac ttaaatcgcc tttattagat
     1321 acggcagtaa tttgtagctt ttgctcttca cccgcggatt ttgccagaat aaccctggca
     1381 ccattggaaa gtgttaatga tgtcagattc tcagccagcg tttctttgct gctaatttcc
     1441 gcctgtgggt cagcgtctac tgttaacgat aaatttctgc ctgggaagat gtaagccgcc
     1501 agctttttgt tggcgtactc tttttccagc gccaggatag ccgcaggaga caatgctttt
     1561 ttggcggcaa cttcattgtt taccatttgc tcccaaaatg cgtcctggtt ctttcttaac
     1621 tgctgccatt tttccgccag actttgcacg gtaatttgct gccacagacg tttcgaaagt
     1681 tgatatgtct cttccggcga caagaacggc gtattattta atgagctgga tgccaggcga
     1741 ctggtcagca tacgtaaatc acgctcagct tgctgatcaa ccgcattttt cagccaggta
     1801 aggcgggtag atttaacatc atcgagttct tcagcagaaa agccatgctg atcaatggtt
     1861 gccaactctg ccattaatgc attcgcagca tcctgcatat tatcgtcttg tgcatttaca
     1921 cggaaaaaca aagactgata atcgggtgca attttgacgc tgcgcgcagt gccgccagaa
     1981 atagtcttca actcgcccga ctgtatgcgt tcttgcagac gttggttgaa cagctgaacc
     2041 aacatgctcc attcagcttg ttcgataaag ctttgctcat cgttcacttg taccattggc
     2101 aggcgataat agagtgcgat gccgttcacc cggttttctt tatcattgat gatattaaag
     2161 cgcaggtggt tttcggcttt tgtcggccag acgcgatttt cagctgcttt gttagacgga
     2221 agcttactta aattatcctt aatcagcgcc agcgcttctt tactgtcaat atcgccgacc
     2281 acgataaagg tcatattatt tggttgatac cagcgttgat aaaattggcg caattgtgcc
     2341 ggtgtgaccg tagcgacagt atccatcagg ccgataggtt cacggtctaa attacgggta
     2401 tttgccagca ggaaagggcg gcgcgcctga gaggtgcgcc atttcgcatc ctgatgggca
     2461 cgccattcct cagtaattac gccgcgttca gcgtctactt cgagtttttc aaaggttgcg
     2521 gcattactcc attcactgaa gattgccatc acttgttgca gattttgttt ctgcgtagtc
     2581 ggcaaactca cctgatacac tgtttcgtca tagctggtat aggcattaac atcgcgacca
     2641 aaacgcaggc ccattgactc aaatgtttcg atgactttat tacccggcca tgtttttgtg
     2701 ccgttaaaca tcatatgttc tacaaaatga gccacgccgc gctcattgtc ctcttcctgc
     2761 aatgaaccgg tatgaatttg cagccataaa tttacctgat cctttggttg agcatgcgga
     2821 taaatcatat atcgcaagcc attgtccagt tgcccggtaa ttaacttttc atcctgcggt
     2881 aaggcggcgg caatcagtcg cccggggagc aacagagttg ccactaacat cagtaagaaa
     2941 cagaggtttc tcataattat ctccatgcga aaaccgggca aatttacccg gtaaagtaaa
     3001 atccgaacta ttaaaatttc atgctgacat caagccagaa agtacggcca ctggcgtagg
     3061 tcgccacgcc ggtattcgtt gtatcaaccg cggttttgct atccagtaca ttgagaatat
     3121 cggcactgat cgtcaggttt tgttgtttca gaaattgtgg cgtccaggac aagcgggtgt
     3181 cccaggtcag gctgctatcc agtttctcgt caacatactg cttgtaatct gaatattcgt
     3241 tgatgtattg cgcattcgtc ttacccagga taatgcgagc tttacgcgct tcttgccagg
     3301 tcagcgtatt cgcccacacc aacccgctcg gttgatgcgt gaaatccatg tttaaggaga
     3361 tctttaatgg attattaaaa tccgccactg gaacgctatc gtaagagacc agattaccgt
     3421 tataaaccac ccggttatca tcggtattgc tttcctcata accattgttt aacgacaagt
     3481 tgcccttgct cttgatatag ctaaagacaa tttgcgggtt aacatctacc tggctgatat
     3541 gcaggggttc ggccagttca aaactgaggt tgaacgaatg cgttttggtt ttgccgtcgt
     3601 tgttatattc agtaatggtg gttttagtcg cgctgtcggt acgactgctt ttgctgattt
     3661 gatcatgcgc ttcacggtaa acatagtttg cgcgcgcaat aacgttctta ccgattttct
     3721 gctgcaaccc cattgccagt tcatcgttat aaggcgtttt caaatcctga taacgcgtca
     3781 gggttttatt gcctgatacc gattccgtcc agctattgcg gatatcacgt aatcccatat
     3841 caagaatatt cccgccatag taacggttat aacctgcggt aatcattgag gtttgatcag
     3901 caaaaatatc ccattccgtc ataaagcgcg gggagatatt gtggtttgac agatagttgt
     3961 catagtcata ccgcacaccg ggcattaacg acacattccg ccagctaatg cgatccgcca
     4021 tatacagcgt gtagttgtca attcccagac tgcctttacc tttatggtaa atggtatggt
     4081 tagttttctt tcctgcagca ttaatcacat aggattcaga ctggttatgg cgttcagtcc
     4141 atgcatcgga atagatgtat tccgcgccga agtagggttg atgcgaaaca tcacccacgg
     4201 cgaatttttg ccagtccagg cgtgttttga aggtgtaatt atctacagcc tgggaaatgt
     4261 gtcctaatcc accacgggtg caacgacctg aaatatcacc atatgtacat gaaagttcgg
     4321 tgtaccagat gtcatgatcg tgacgggtat aatcactaat atgatcccaa ccaacggtag
     4381 tacgtagttt ggcccatgcg agctgcgtat ccatatccca tgccagaccg taggatttat
     4441 tacccatttc gcgatcagac tgcgggaagg tgctggtatt ataatcacga ctggagccgg
     4501 tatattttaa ggtaaaatcg tgggtaaagc ggtcgctggc aaaccaagta aatttgctca
     4561 atgcggtatc gataacgttt ttatactgtg cccgaccggc gacaatgccg tcattcgaaa
     4621 cataatccgc gcgggtgata tcagactggc ggcgcgataa accggcggta acgccaaagt
     4681 tatcagcgag ttcctgatta aacgacaagg tataaaagtt ctttttaaaa tctggagagt
     4741 aataagtact tcctgaagaa ccttgattaa atgcgctctt gttattctca tcgatatgcg
     4801 atgttaacca gtccgaacgc gtagtgcgat aacccagttt cacgctgcta tcatcagcgt
     4861 tgaagcgttt gatctttgca tcaattaccc cgccattgaa gcgaccgaat tcaaccggca
     4921 caaaactgtc ataaagcgtc acattgtcca gtaagctgac atcaagataa tagccctgtg
     4981 acatcccgct aatattggtt gcactactgg catcggactc attcgctggg ttcaggttat
     5041 tagttgcact aataccatca atcaaatagg cattctggta gggcgacgca ccgtgaatag
     5101 agattttctc cgggcgaata tctccctggt tcaatgaggt actttgcgtt gaatccatgc
     5161 gcaccgcagg gttggttctc agcagatcgc tgatattgcc attcccggtc ggtaaacgct
     5221 cgatgctttc actggtgtaa tgcgtgttgc cgtttacggg tacagggaca ggagcataga
     5281 ccgtcatctc ttcaaaaaca tgcatgtaca tgtttttgtc atcgacggcc tgcgctatat
     5341 cagcgccaca taaaatgacg ccaggaataa gaactcgctt catttattta tatctccgta
     5401 cagcagggct tttattgttt ttatataacc gcgctaatgt cacaaatatc atcagccagg
     5461 ttccagacac cgggttgatg tgtaaccata atgacgccgc ttgtgggtag tttttcacgc
     5521 actaaacgca gtaagcggat agcctcttgt tcctcaagat gagaggtagt ttcgtcaaga
     5581 aatatccatt tcggacgtcg taaaattaat cgtgccaggg cgatacgttg tttttcgccg
     5641 ctggaaagaa tatcgcccca gcgatcgtgg tcatgaatac gcgcagccaa tttccccaga
     5701 ccaacctgat gcagtacttc gctcaacgat ttatcgtcta cggacagggg aagtgcttta
     5761 caaattatct ctttcagtaa gccggttttg attaacggtg tttgtgacac ataccaactg
     5821 tcagcaggag aagaaatatc acctttaaac cacggccagc agtgggataa tgttttaagc
     5881 agtgtggttt ttcccgcacc agagtaacct ttcagtaata gccatttgcc tggcgaaaca
     5941 tgaaagttca ggttctctaa tatgatctta ttatcaggag tacgaatact cgcgtcagcc
     6001 acttgtaccg catgttggca atttttaggc ttattcgtag ggcgctgttc ggtgagttga
     6061 tggaactcat acaaacgatc gataaccgca gccagttcag caagttcgtc atatttataa
     6121 ataaaccagc ttaaattgtt cgataccagc ataaatgcct ggcgcgattt catcagtccg
     6181 cccagattga tctgtccgct aataaactgc ggcaataaca gaaagtaggg aagaacgcta
     6241 agcgagcgcg aataaatatt ctgccagtaa tcaagccacc gctgacgatt cattaaacga
     6301 tgccagttct ctttaatggt atgaaaattg tcgcttaact cctggcgttg tagatactca
     6361 gcattgctta gcgcaatcag ttctgcctgc ttattatgct gcacaagatt agttcgaaat
     6421 gtcgcttcgc tacgttgttt ttccacatta agcggacgaa tacgtttacc aaccttatgg
     6481 gtaaataaag ttccaccgat cacaatgagc acaacggtat agaccatata cccctggata
     6541 ttccattctg ttccaccaac agtgaatgag agcgtacccg cgctttgcca aagaataacg
     6601 gtaaaggtga tcaacatgct aagtgactgg atgaagccaa atgaaaggct gagcgtttta
     6661 ctgatcagta agagaatgtc ctcagcgata cgttggtcag ggttatctgt atttttatgc
     6721 tcaccgtaga tctgcgtgaa gtagtagttt ttatctgcaa accagcgatt aaggtagtaa
     6781 tctgttaacc attcgcgcca gcgaatagtc agtagtttaa ttagccatgt tttgtttacg
     6841 gatattaata caaagatccc cagcagggca gggaaccaaa ggacaagctg ccaaagctta
     6901 tcggtttctt tctggcttag cgcattgaaa aaatcattat tccagtcgtt taaccagacc
     6961 tgaattttaa ccacgccaag gatcatggcg aggataataa tgattaacaa cactgaagtt
     7021 ttattattct tacgcagcca aaatggcttc agcagacaga gatactttgc tatcaacata
     7081 caaaacgtaa tgggaatggt tatcattagc gaaaattgat tgtgccatcc aatgattatc
     7141 tatggcaatg
//
LOCUS       DAZGTJ010000161         7068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000161 DAZGTJ010000000
VERSION     DAZGTJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.161"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..131
                     /locus_tag="ACHK8E_004844"
     CDS             <1..131
                     /locus_tag="ACHK8E_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0346988.1"
                     /translation="RHIAINILTNDKVFKAGLRRKLRKAAMDRNYLASVLAGSGLS"
     gene            329..2566
                     /gene="nrfB"
                     /locus_tag="ACHK8E_004845"
     CDS             329..2566
                     /gene="nrfB"
                     /locus_tag="ACHK8E_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP0346989.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            2553..5525
                     /gene="nfrA"
                     /locus_tag="ACHK8E_004846"
     CDS             2553..5525
                     /gene="nfrA"
                     /locus_tag="ACHK8E_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP0346990.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5526..6416
                     /gene="ybcH"
                     /locus_tag="ACHK8E_004847"
     CDS             5526..6416
                     /gene="ybcH"
                     /locus_tag="ACHK8E_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP0346991.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         1774 a         1820 c         1810 g         1664 t
ORIGIN      
        1 tacggcacat cgctattaat attttgacga atgataaggt attcaaggca gggttaagac
       61 gtaagttgcg aaaagcagcc atggacagaa actatctcgc gtcagtcctt gctgggagcg
      121 ggctttcgta atctttccct gcgttaacct cccctccttg acaccacgct tttgtctaca
      181 ttctacgcaa acggcaactt ttataaccag tgtaaaaata acgtgccgca atagtcatac
      241 ctattaatgg taaaaagctg tcacaattca taaaaaacct taatatacgc caccctaaac
      301 ataaccagcg ttaatgtaag gtttttgtgt ggactggctt cttgatgttt ttgctacctg
      361 gctctacggc ttaaaagtaa tcgcgataac gttagcggtc atcatgttca tcagcgggct
      421 ggacgatttt tttattgatg tcgtctactg ggtacgccgc attaaacgca agttgagtgt
      481 ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat aaaccagatg aaaaaccgtt
      541 agcgattatg gttccggcgt ggaatgaaac gggcgtcatc ggcaatatgg ccgagctggc
      601 ggcgaccacg ctcgactacg aaaactatca tatctttgtt ggcacctacc ccaacgaccc
      661 cgatactcag cgtgatgttg acgaagtgtg cgctcgcttc ccgaacgtgc ataaggtagt
      721 ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg aacaacgtgc tggacgccat
      781 cacccaattt gaacgtagcg ccaatttcgc ttttgctggt tttattctgc atgacgccga
      841 agatgtgatt tcaccgatgg aattgcgtct gttcaactat ctggtcgagc gtaaagatct
      901 gattcagatc ccagtgtatc cgttcgaacg cgaatggacg cacttcacca gcatgaccta
      961 cattgatgag ttttcagaac tgcatggcaa agatgttccg gtgcgtgaag ccctcgccgg
     1021 acaggtgccc agcgcaggcg tcggcacctg tttcagccgc cgcgccgtga ccgcactgtt
     1081 agctgacggt gacggtattg ctttcgacgt gcagagtctg actgaagatt acgacattgg
     1141 tttccgcctg aaagaaaaag gtatgacgga aatttttgtc cgttttccgg tggtggacga
     1201 agccaaagaa cgcgagcagc gtaaattttt acagcacgcg cggacgtcaa acatgatctg
     1261 cgtgcgcgaa tatttccccg ataccttttc gactgcggtt cgacaaaaat ctcgctggat
     1321 catcggcatt gttttccagg gctttaaaac ccacaaatgg acctccagcc tgacgctgaa
     1381 ctactttctc tggcgcgacc gcaaaggggc aatcagtaac tttgtcagct tcctcgcgat
     1441 gctggtgatg atccagcttt tgctgttact ggcgtatgaa agtttgtggc ccgatgcctg
     1501 gcatttcctt tctattttca gcggcagcgc atggttaatg accctgctgt ggctaaactt
     1561 tggtttgatg gttaaccgca tcgtgcagcg ggtgattttt gtcactggct actacggtct
     1621 gacgcagggg ctgctatctg tcctgcgtct tttctggggc aacctgatta acttcatggc
     1681 caactggcgc gcgttaaaac aggtacttca acacggcgat ccacgtcgcg tcgcgtggga
     1741 taaaacaacg catgacttcc ccagcgtgac tggcgatacc cgctcgttgc gcccgttagg
     1801 ccaaatcctg ctggaaaatc aggtcatcac tgaagaacaa ctcgatacag cactgcgtaa
     1861 tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg caggggctga ttagcgccga
     1921 acagctggca caggcgctgg cagagcaaaa cggcgtggcg tgggagtcga tcgacgcctg
     1981 gcagatccct tcatcactga ttgccgaaat gccggcttcc gtggcgctgc attatgcagt
     2041 gctgccgctg cgtctggaaa atgatgagtt aattgtcggc agtgaagatg gtattgaccc
     2101 ggtttcgctg gcagccctga cgcgtaaagt cggacgcaaa gtgcgttacg tcattgttct
     2161 gcgcggacaa attgtcacgg ggttacgtca ctggtatgca cgccgacgcg gtcacgaccc
     2221 gcgggcaatg ttgtacaatg cggttcagca tcagtggctc acggaacagc aggccggtga
     2281 aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc gaaatactga ccacgctcgg
     2341 tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc catgaacgca gttctctgcc
     2401 gctcggcaag tttttggtca ccgaaggcgt tatcagccag gaaacgttgg atcgcgtcct
     2461 gacaattcaa cgcgaattac aagtttcgat gcaatcacta ttactcaaag caggtttaaa
     2521 cccagaacag gttgcgcaac tggagtccga aaatgaagga gaataacctt aatcgcgtca
     2581 tcggatggtc tggtttactg ctgacgtctt tattgagcac cagcgcactc gcagacaata
     2641 tcggcaccag cgcagaagag ctggggctga gcgattatcg ccattttgtt atttatcccc
     2701 gtctcgataa ggcgctgaag gcacagaaaa ataacgacga agcaaccgcc atccgcgaat
     2761 ttgaatatat acaccaacag gtgccggata atattccgct gactttatac cttgcggaag
     2821 cctatcgcca ttttggtcat gatgaccggg cgcggctgtt gcttgaggat caactgaaac
     2881 gtcacccagg agatgcccga cttgagcgca gtctggcggc tattccagtt gaagtgaaaa
     2941 gcgttacgac tgttgaagaa ctgcttgccc agcaaaaagc gtgcgatgct gcgccgaccc
     3001 tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct ggcacaatta cctgtcgcca
     3061 gagcgcaact gaacgatgcg acgtttgctg catcgccgga aggaaaaacg ctgcgaaccg
     3121 atctgctgca acgggcaatc tacctgaaac aatggtccca ggcagatacg ctatacaatg
     3181 aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg tcagtggttt gatgtgcttc
     3241 ttgccgggca gctggacgat cggatcctgg cgctgcaatc acaggggatc ttcaccgatc
     3301 ctcagtcata tattacttac gcgaccgcgc tggcttatcg tggcgaaaaa gcacgcctcc
     3361 agcattatct cattgaaaat acgccgctat ttaccacgga cgcacaagag aaaagttggc
     3421 tctatctgtt atctaaatac agcggcaacc ccgttcaggc gttggcgaat tatacggtac
     3481 agtttgccga caaccgccag tatgttgttg gcgcgacgct accggtgctg ttaaaagaag
     3541 gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc cgccaatgaa atgcttgagg
     3601 agcgttatac cgttagcgtg gcgacccgta acaaggctga agctctgcgt ctggcacgat
     3661 tgctgtatca gcaagaaccg gcaaatctta cccgcctgga tcaactgacc tggcaactga
     3721 tgcagaacga gcagtcacgt gaagctgccg atttattgct gcaacgctat cctttccagg
     3781 gcgatgcgcg tgtcagccag actttaatgg cgcgactggc gtctctgctg gaaagtcatc
     3841 cttacctggc aactccggcg aaggtggcga ttttatcgaa acccttaccg ctggcggagc
     3901 aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa ttgcccggca atagttcgct
     3961 tgctgggcga tatgtcgcct tcctacgatg ctgccgcctg gaaccgtctg gcaaagtgtt
     4021 atcgggacac gctacccggt gtggcgttgt atgcatggct tcaggccgaa caacgacaac
     4081 cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta tcaggttgag gactacgcca
     4141 ccgcactggc ggcctggcag aaaatcagtc ttcacggcat gagcaatgag gatctgcttg
     4201 ctgctgccaa taccgcccag gcggcaggaa atggtgcggc tcgcgatcgc tggcttcagc
     4261 aggcagaaca acgtgggctg ggaaacaatg ccctctactg gtggctgcat gcgcaacgtt
     4321 acattcctgg tcagccggaa ctcgcactga gcgatctcac gcgctcaatc aatattgcgc
     4381 cttctgccaa cgcttacgtt gcgcgggcga caatttatcg ccaacgtcat aatgtcccgg
     4441 cggcggtgag tgatttgcgc gccgctctgg aactggaacc gaataatagc aacacccagg
     4501 cagcgctcgg ttacgccttg tgggatagcg gtgatatcgc acagtcgcgg gaaatgctcg
     4561 aacaggcgca taaagggcta ccggacgatc cggcacttat ccgacaactg gcctacgtga
     4621 accagcgtct ggatgacatg cctgcgacgc agcactacgc ccgactggtg attgatgaca
     4681 ttgataatca ggcgctgata accccactga ccccagagca aaatcagcaa cgcttcaatt
     4741 tccgccgtct gcatgaggag gtcggtcgcc actggacgtt cagtttcgat tcttctattg
     4801 gcttgcgttc cggcgcaatg agtaccgcta acaataatgt cggcggcgca gcgccaagga
     4861 aaagctatcg tagctacggg caactggaag ccgagtaccg catcggacgc aatatgctgc
     4921 tggaaggcga cctgctctca gtttatagcc gcgtctttgc cgataccggt gaaaacgggg
     4981 tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct gcgctggaag ccgctgcgcg
     5041 atcagatctt tttcctcgcc gtcgaacagc agttgccgct gaacggacaa aatggcgcat
     5101 ccgataccat gctgcgcgcc agcgcctcat tctttaatgg cggcaaatac agcgacgaat
     5161 ggcacccgaa cggttcaggc tggtttgccc aaaacctgta cctcgatgcg gcgcaatata
     5221 tccgccagga tattcaggcg tggacggcag attatcgagt cagctggcat cagaaggtgg
     5281 caaacgggca gactattgag ccttatgctc acgttcagga caacggttat cgtgataaag
     5341 gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg gaatatctgg accggcgaga
     5401 cgcactacga cgcctggccg cacaaagtca gtctcggcat cgaatatcaa cataccttta
     5461 aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt tctcaccatt ggagtgcact
     5521 ggtaaatgcg taagttcatt ttcgtattgt tgacactgct tttggtcagc cctttttcct
     5581 ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga tagtcaggtt accgataccc
     5641 agtggcaggg gctgatgagt cagttacgtt tgcaaggctt cgataccctg gttttgcaat
     5701 ggacccgtta cggcgatgca ttcacccagc cagaacagcg cgcgttattg tttaagcgtg
     5761 ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg gctgaacgcc gatccggaat
     5821 tttttataca ccagaaacag tcctccgcag cgctggaaag ctatcttaat cgcctgctgg
     5881 ctgccgatct ccagcaagcc agattatgga gcgccgtgcc tggcgtaacg ccggatggct
     5941 ggtacatcag cgcggaaatt gacgacctga actggcgcag cgaagccgcc cgtcaacctt
     6001 tgctaacatg gttaaacaac gcgcagcggt tgattagcga tgtttcagca aaaccggttt
     6061 atatcagtag ttttttcgcc ggaaacatgt cgccagatgg ctatcgccaa ctgctggaac
     6121 aagttaaagc aaccggtgtt aatgtctggg tacaggatgg cagcggcgtg gataaactga
     6181 ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga ttgccaaagt tccgccccag
     6241 caagcggcat tgtttatgaa ctgtttgtcg ctggtaaagg taaaacgttt acagcgaaac
     6301 cgaaaccgga tgcagagatc gcctcgctgt tagcgaaacg ttcctcttgc ggtaaagaca
     6361 cgctctattt ctctctgcgc tatttgcccg tcgcgcagag tatccttgag tattaatgat
     6421 taaagggagc aacttagctt gctcctttcc ttttcacttt cctaatcaaa atcctggcaa
     6481 gatgaattgt ttagcagaat ttctgttttc attttattcg tctgacgtgt tccaattatt
     6541 tagcaaaacc gcgacaacaa agtgatggtg taatttgtaa aagggaacaa ataaatggcg
     6601 aggaaatggc tcgccctgca ggattcgaac ctgtagccca cgacttagaa actccaggaa
     6661 cgacatttta aatcaacaaa ttaccgcgcc atctctgcgt tcacacgttc caccacctca
     6721 aaatatgtaa aaccttgcaa gccattgcga ggccttatgt gtctcagttt tgttcctctt
     6781 ttttatacta aaaaacacag caattgagga taaacctcat gctattatcg ctaatatgca
     6841 tctaaaggca tggtacttga atagataaaa actccacaaa gaaaaaaata attagcccat
     6901 aaaaccacaa tgcaaaacac aaacagacaa ttaaactaaa aaactgacag tgcattgtga
     6961 caaaaattca atgttaaaca aaatataaac agtggggcaa tatacaattg actcattaag
     7021 ttagatataa aaaatacata ctcaatcatt aaaacgattg aatggaga
//
LOCUS       DAZGTJ010000162         6778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000162 DAZGTJ010000000
VERSION     DAZGTJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.162"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..867
                     /locus_tag="ACHK8E_004848"
     CDS             <1..867
                     /locus_tag="ACHK8E_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0346992.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLITEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMNFGKGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAE
                     GYNGQATLNVTF"
     gene            938..1171
                     /locus_tag="ACHK8E_004849"
     CDS             938..1171
                     /locus_tag="ACHK8E_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0346993.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            1261..2079
                     /locus_tag="ACHK8E_004850"
     CDS             1261..2079
                     /locus_tag="ACHK8E_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0346994.1"
                     /translation="MRLASRFGYANQIRRDRPLAREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            2134..2619
                     /locus_tag="ACHK8E_004851"
     CDS             2134..2619
                     /locus_tag="ACHK8E_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0346995.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIVACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2635..3111
                     /locus_tag="ACHK8E_004852"
     CDS             2635..3111
                     /locus_tag="ACHK8E_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0346996.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3174..3395
                     /locus_tag="ACHK8E_004853"
     CDS             3174..3395
                     /locus_tag="ACHK8E_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0346997.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            3558..3932
                     /locus_tag="ACHK8E_004854"
     CDS             3558..3932
                     /locus_tag="ACHK8E_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0346998.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            3979..4356
                     /locus_tag="ACHK8E_004855"
     CDS             3979..4356
                     /locus_tag="ACHK8E_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0346999.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            4353..4841
                     /locus_tag="ACHK8E_004856"
     CDS             4353..4841
                     /locus_tag="ACHK8E_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0347000.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            4853..5050
                     /locus_tag="ACHK8E_004857"
     CDS             4853..5050
                     /locus_tag="ACHK8E_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0347001.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            5135..5980
                     /locus_tag="ACHK8E_004858"
     CDS             5135..5980
                     /locus_tag="ACHK8E_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0347002.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
     gene            6573..>6778
                     /locus_tag="ACHK8E_004859"
     CDS             6573..>6778
                     /locus_tag="ACHK8E_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347003.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNVIYLFEN"
BASE COUNT         1673 a         1823 c         1699 g         1583 t
ORIGIN      
        1 catctcggtc acgataacaa cggcggtatt gcccgtgggg ccacgccgga aagcagcggc
       61 agctatggct tcgtccgtct ggagggtgac ctgctcataa cagaggtcgc cggtatgtca
      121 ctgacgactg gggtgtatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
      181 ggctcccgcg ccggcacggt ccgggatgat gccggcagcc tgggcggata tatgaacctg
      241 acacacacct cctccggcct gtgggctgac atagtggcac agggaacccg ccacagtatg
      301 aaagcgtcat cgggcaataa cgacttccgc gcacggggct ggggctggct gggctcactg
      361 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtac
      421 acctggcagg gactttccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      481 catggcagtg cacaacatgt gcgtgccggc ttccgtctgg gcagccacaa cgatatgaac
      541 tttggtaaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca cagtgtgcgt
      601 gaactgccgg tgaactggtg ggtacagcct tccgttatcc gcaccttcag ctcccggggt
      661 gatatgcgtg tggggacctc caccgcaggc agcgggatga cgttctcacc gtcccagaat
      721 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
      781 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg ctataacggt
      841 caggccacgc tgaatgtgac tttctgataa tcaggtcact ccccttcagg ccccggtcat
      901 catgaccggg gctttttact ttcagggagc aacagatatg ccgggttgta cagcacgttt
      961 attgcccgaa ggccctttct cacgcggaca ggctgttgcc gtcacaacag cttaccgcaa
     1021 tgtgtttact gaagatgacc agggaacata tttccggctg gttatccgta atgccgaagg
     1081 gcagctacgc tggcggtgct ggaattttga gtctgacgcc ggaaaacagc ttaatgtgtg
     1141 gctctccagt gaagggcttc tcaggcaata aacgtcttca tttcatccat caggccgcat
     1201 cttctccggg agacgcggcc ttttcattta taccgccaac tcattcataa ggagcaaatc
     1261 atgcgattag caagtcgttt tggttatgcg aaccagatac gccgtgaccg tccactggca
     1321 cgcgaagaac tgatgtacca cgtcccgagt atttttggag aagaccggca cacctcccgc
     1381 agtgaacggt atgcgtacat tcccaccatc accgtcctgg aaaatctgca gcgggaaggc
     1441 tttcagccgt tcttcgcctg ccagacccgt gtgcgcgacc agagccgccg ggaatatacc
     1501 aaacacatgc tgcgtctgcg gcgggccgga cagataaccg gtcagcatgt gcctgaaatt
     1561 attctgctca actcccatga cggtacctcc agctaccaga tgttacccgg atattttcgt
     1621 gccatttgta ccaatggcct ggtctgcggt cagtcgctgg gagaagtccg ggtgccacac
     1681 cggggaaacg tggtggacag ggtcatagaa ggcgcttacg aagtggtggg cgtgtttgac
     1741 cggattgagg aaaagcgtga tgccatgcag tcgctggtcc tgccgccacc ggcacgccag
     1801 gcgctggcac aggcggcact gacttaccgt tatggtgacg aacatcagcc cgtcaccacc
     1861 gccgacattc tgacgccacg acgccgggag gattacggta aggacctgtg gagtgcttat
     1921 cagaccattc aggagaatat gctgaaaggt ggaatttcag gtcgcagcgc taaaggaaaa
     1981 cgtatccata cccatgccat tcacagcatt gacactgaca ttaagctcaa ccgcgcattg
     2041 tgggtgatgg cggaaacgct gctggagagc ctgcgctgat tcagccatta ccctgaaaac
     2101 cacgcattta tttgtccttt aaggagtcat ttcatgaaaa ccatttcaca taattccacc
     2161 acatcttctg tttccgtaac cgctgcatca gggaatgacc agtctcagct ggttgccact
     2221 cccgtccctg atgaacagcg catcagcttc tggccgcagc attttggcct cattccacag
     2281 tgggtgaccc tggagccccg tatcttcggc tggatggacc gtctttgcga agactactgc
     2341 ggtggtatct ggaatctgta cacactgaac aacggtggcg catttatggc ccccgaacca
     2401 gatgacgatg atggcgaaaa ctgggtactg ttcaatgcca tgaacggtaa ccgcgctgaa
     2461 atgagcccgg aagccgccgg cattgtcgcc tgtctgatga cgtacagtca tcatgcctgt
     2521 cgtacggagt gttatgccat gacggtccat tattaccggt tgcgggatta cgccctgcag
     2581 catccggaat gcagcgccat tatgcgcatc atcgactgaa tggagagaag cacaatgcaa
     2641 caactttcct ttctgcccgg agagatgacg cccggcgagc gcagtctcat tctgcgggcc
     2701 ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg ccttcacctc cacccgtgcg
     2761 gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagaatt ccgggtgctg
     2821 tatctgaata accagaatca gttgattgcc ggtgaaaccc tcttcaccgg caccatcaac
     2881 cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc
     2941 gtggtgctgg cgcacaatca cccgtccggt gaagtcacac ccagcaaggc agaccgcctt
     3001 atcacggaac gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg
     3061 atagtcggtg gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccgtcaca
     3121 accacatcac gcttgttttc acttttatct tctgtcttca gaggtatcac actatgaaaa
     3181 tcatcacccg tggtgaagcc atgcgtattc accaacaaca tccggcatcc cgtctttttc
     3241 ctttctgtac cggcaaatac cgctggcacg gcagcactga agcgtatacc ggtcgtgaag
     3301 tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg ccataaggac agttttggcc
     3361 cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg aaaacgagta
     3421 cgaccagatg gactcagacg ccgctgtgga tgaggccctg ctcatcaacg aatacacctt
     3481 taccgaagca ggtcagcgcg ccggctgacc tgtcatcgtt aaaccattct taaccacttt
     3541 tcagagagga ttttatcgtg tcagacaaac tctccgggat aactcatccc gacgacaatc
     3601 acgaccgccc ctggtggggg cttccctgca cagtgaggcc ctgttttggt gcccgtctgg
     3661 tgcaggaggg taaccgcctg cattaccttg ccgaccgcgc cggtatcaga ggccgcttca
     3721 gcgacgcaga tgcataccat ctggaccagg cctttccgct gctgatgaaa caactggagc
     3781 tcatgctcac cagcggtgaa ctgaatcccc gaaatcagca taccgtcacg ctgtatgcaa
     3841 aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg ttacgtttat ctggctgttt
     3901 atccggcaca ggcacccgca accacctcat aaccccgaag ctctcacctt tagcactacc
     3961 agttacagag aacttaagat gaaaacatta tccgacacac atgtacggga ggtatctcgc
     4021 tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc gactgctgga ccagcattat
     4081 ggcctcacac tgaatgacac accgtttgcc gatgaacgtg tgattgagca gcatattgaa
     4141 gctggcattt cactgtgcga tgcagtgaac tttcttgttg aaaaatacgc actggtgcgt
     4201 accgaccagc cggaattcag tgcctgtacc cgttctcagt taataaacag catcgatatc
     4261 ctccgggctc gcagggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac
     4321 attacccagg ggaagcatcc ggaggcgaaa caatgaaact gactctgacg ctggaagcag
     4381 acaacgttaa cgtgcaggcg ctgaacatgg gacgaattgt cgttgacgtc gatggtgtta
     4441 atctcgctga actgattaac gtggtctgcg acaatggcca ctcccttcgt gttgttgatg
     4501 aatctgaccg ggtctcaaca gactgcacgc caccatttgc tgcccttacc ggcatacgct
     4561 gcagtaccgc acatatcacg gaaacggaca acgcctggct gtactcgctg tcacaccaga
     4621 ctaatgacga ctgcgaaaca gaatggattc attttacagg cagcggatat ctgttacgta
     4681 ccgatgcgtg gtcatacccg gttctgcggc ttaagcgcct gggcctgtca aaaacgttcc
     4741 gtcgtctggt tgtcacactt acccgacgtt atggcgtcag tctcattcat ctggatgcca
     4801 gcgctgaatg cctgccgggt ttacccactt tcaactggta accaggaaca acatgaaatc
     4861 attaaccacg gaaacagcac tggatattct gattgcgtgg ctgcaggaca atatcgactg
     4921 cgaatcggga attatctttg acaacgatga ggataaaacg gattcggcag cactgctgcc
     4981 ctgcattgaa caggcgaggg aggatatcca taccctgcgc caggtgcagc ttctgcacca
     5041 gaaccggtga gtcttactca ccagacttca ttccactgac gccagcctga acagggctgg
     5101 cgttttcatt tatctgcgaa aaggaatatc gattatgtct gcaatcacag tctcccgtcc
     5161 ggaagtggtc accgggcata cggacgttat ctgctccacc tcagtcagtc acattctggc
     5221 tgtgcgaaag actacactgc tgcaaatcga cacacttatc cggcaactgg ctgaaatctc
     5281 ggtactgaca gaaagtattg gaggtaaaac tgctctggac tgggccatga aacaggattt
     5341 tcgctgcggc tgctggctga tggagaaacc tgaaaccgca atgaaagcta tcacacgcaa
     5401 tctcgatcgt gaaatctggc gtgacctgat gcaacgttcc gggatgcttt ccttaatgga
     5461 tgcacaggcc cgtgatacat ggtaccggtc actggaatat gataattttc cggaaatcag
     5521 cgaggcgaat atcctgagca catttgaaca actgcaccag aataaggatg aggtgtttga
     5581 gcgaggggtg atcaacgtct tcaggggact gagctggaat tacaaaacca attgcccctg
     5641 caaatttggc agtaaaatta tcgtcaacaa cctggtgagg tgggactggt gggggtttca
     5701 tcttatcacc gggcaacagg cagatcgact tgccgatctg gaaagaatgc tacacctgtt
     5761 cagcggcaaa ccgatccccg acaaccggga aaacatcacc cttcgtctgg atgatcacat
     5821 ccagtctgtt cagggtaaag agtgctatga agatgagatg ttcagcatca gatactttaa
     5881 gaagggctcc gcgcacatca cgttcaaaaa tcaagaactg gttgacaggc tgaatgacat
     5941 tattgcaaga cactatccga gaatgttacc ttcattataa agttctaaga atgaattcaa
     6001 ttttgcattt tagcttcatt ccctcacgca aaattatttg ggggttctta tgggggttac
     6061 tcatttatcc aacacaaatt aatcatttaa aattaatata ttaatactca tattcgactc
     6121 ctgtgatcgc caaaatgcct cttcagtgat ccccatcctg cttatttctg agcaacctga
     6181 ccctgctgat gtatctggta gtatgacaga gtgattcatt tttcttgccg agtgtacgaa
     6241 aacgtcttcc ttgccaccga ctatcaaccg tcactcaaaa aaacctctct ttttttcaag
     6301 ctatcaagaa taagttgcat tttatctgca ccggacacca gccatccata tatggctttt
     6361 acctgctaca aagatgaaga agtgcataaa tgtgatgtta aaacaatcaa agaaaaatta
     6421 catgtgcaat aatttcacta taacattata atccatggtg aaaataacac tgcacaagga
     6481 ttgtcatcat caaactcatc ggaactattt tttctgtcgt cattggtgta atactgattg
     6541 taagtatcct ttcaggagat tatttttgcc gtgtggggca tggaacttgt aaagcagcca
     6601 aaagcgctag gcatttaatt gaaagtaaag agaaaaacag aagtagtcgc gtcaaagtct
     6661 ttagcacgca tattaatttg agtcatccag cggcatcctg gctagcaact caaacttgcg
     6721 ctaacaatat attcaatcta cttcaaaaca ataatgtcat ttatttattc gaaaacga
//
LOCUS       DAZGTJ010000163         6696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000163 DAZGTJ010000000
VERSION     DAZGTJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.163"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..160)
                     /locus_tag="ACHK8E_004860"
     CDS             complement(<1..160)
                     /locus_tag="ACHK8E_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP0347004.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVL"
     gene            280..480
                     /locus_tag="ACHK8E_004861"
     CDS             280..480
                     /locus_tag="ACHK8E_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347005.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            520..702
                     /locus_tag="ACHK8E_004862"
     CDS             520..702
                     /locus_tag="ACHK8E_004862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347006.1"
                     /translation="MFFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEANFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            695..1561
                     /locus_tag="ACHK8E_004863"
     CDS             695..1561
                     /locus_tag="ACHK8E_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0347007.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLKNMDAGELFFWQVGSAAM
                     LSWFVLAISRKKIPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITSVIFSLESV
                     FILILSVIFFSVKINSLLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFTLACFFIVLTDTSLQLPIESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
     gene            2046..2651
                     /locus_tag="ACHK8E_004864"
     CDS             2046..2651
                     /locus_tag="ACHK8E_004864"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0347008.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            2648..3565
                     /locus_tag="ACHK8E_004865"
     CDS             2648..3565
                     /locus_tag="ACHK8E_004865"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0347009.1"
                     /translation="MTLLEKSLVVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            3569..4210
                     /locus_tag="ACHK8E_004866"
     CDS             3569..4210
                     /locus_tag="ACHK8E_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP0347010.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLTQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQKLTALLQPSQGNHPHD"
     gene            4203..5963
                     /locus_tag="ACHK8E_004867"
     CDS             4203..5963
                     /locus_tag="ACHK8E_004867"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP0347011.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPLITRPIAVNILLDLYKTTERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPGADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSAVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            5960..6231
                     /locus_tag="ACHK8E_004868"
                     /pseudo
     CDS             5960..6231
                     /locus_tag="ACHK8E_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6253..6509)
                     /locus_tag="ACHK8E_004869"
                     /pseudo
     CDS             complement(6253..6509)
                     /locus_tag="ACHK8E_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1550 a         1565 c         1690 g         1891 t
ORIGIN      
        1 tcagcaccgt gacctgccag aacggcataa agggtaatgg cggaagccgg atacccagcc
       61 gccagagaat acgcagaaga ggaggggcat aattgctccg ccacatcttt ttgctgtcca
      121 tcagggcaat ggcccgggct tttttctgct caaaggtcac aggagcacct cacgttccat
      181 gatgtaaacg tgacacagct tattgttatt gattattatt atcatgctgt gattatgtta
      241 tcatgcaccg gtacacgata acaataaagg agaaaccaga tgaaactgga tatatctgta
      301 aagtattttc tgaatattat gatagcagat ttcatcattc ccgcaccaga aaaaatatca
      361 accacatggc actcagatac taaaatgaat cgctttgctt gccctataca ttctactaat
      421 agtgattact attcaggttt ttatcataca gatgtattaa ttcccggaga actggcttag
      481 gacgccatca taatccatga tgttgatagt aatgatccta tgttttttaa ctggaatgta
      541 ttgttttatg tctcactgga aaagtttcct gttggcaatc cttattatgg agaaattcca
      601 ggtattcctg tggatgaaga agcaaatttt tttaatccat ttccaccaag agcgtttgaa
      661 aataaacctc tttatactga ggagataact aaatatgatt aagggaatta tgtttgcatt
      721 tcttgctgct ttttcatggg gagcagcaat agtgatgagc aaaaaaggtc tcaaaaacat
      781 ggatgccgga gagttgtttt tctggcaagt tggtagtgct gcaatgttat catggtttgt
      841 tctcgcaatt agcagaaaaa aaatcccggt aacaaaaaaa tcaatacttg cctattcaac
      901 aggaattttt gagccatttc tggcgtatac attcactctc tatggcctaa aatttatttc
      961 tgctggtata acatccgtta ttttttcact tgagtcagta tttattctta tactatctgt
     1021 tatattcttt tccgttaaaa ttaactctct attacacttt atattattgc ttactggagc
     1081 aatggcaggc agtataatgg tcgccctacc tgatatcaat gaccgcattg ataatattac
     1141 aggatatata cttattgttg caggtgtact ctcagcatcc ttttatgttg ttatctcatc
     1201 acgacttatt gtgaattttg agcctgttac gttgttaacc gggcagttga ctttctcttt
     1261 tactcttgct tgttttttta ttgttctaac tgacacatcc ctgcaactgc caatagaatc
     1321 tgtatttctt gtgtttttgt caggaatttt acaatatttt ttggcttttt gtttctatct
     1381 ttattcccta cgctggattc ccgttcacat agcaggagca atgctctatt ttattcctct
     1441 ggtagcattg ctgttgtcct ggttcttcct gcaggaagat atatcattta ttcagggact
     1501 aggcgttttt ataactattt tatgcgtata tattctcaat aagaaatacg ccatcgaatg
     1561 actttattag ggttgcgtta tcgcggtagg gtacaccctg acaccataac caaaaacgtg
     1621 atgccagacg atgaatgaca taaccttaat gcgataaagt ggtaaaaaat gttcatcaga
     1681 gctgatttaa aagaagtaaa gcaatagccc ggaccagcga caaggcatca tgcaacaaat
     1741 gtaacggata ttttgaatat ccttgcctcc gcacatgact gggcataatt aaagaaaagc
     1801 gtatgctgac acagctgcac agagtgctgt accgttgtcc aggacactat gtttatggtt
     1861 cagccacaca gtctaccggc acaggtactg gctctcaggc ctcccgtaat accagtcagt
     1921 cgcctttacc ggcgcagcag gcaggatttc cgcgcccgga actgtattca ctttcagtgt
     1981 gccagtgaca gccccaataa aaggccagac tcaaccccgt caaatatgct tattaaggag
     2041 tgatgatgac cctttaccag ataaagccct cgtttcaggc gctgttaaga ccgctgatgt
     2101 tctggctgaa caggcgcggc gtcaccgcta atcacattac actgtgcgcg atggtgcttt
     2161 cccttatcac cggcgtggtg ctcattattc atcctcagcc cggattattc gtccttctgc
     2221 ccgtggtgtt gtttgtacga atggcgctca atgcgctcga tggtatgctg gcgcgtgaat
     2281 gtaatcaaag atcgcgtctg ggggccatcc tcaatgagac gggtgatgta atttccgaca
     2341 tctcgcttta tctgcccttt atgctactcc cgcagagcag tataccgctg gtgctgatcg
     2401 tggtgttttg tgcgctgatg accgagtttt gcggcgtgct ggcgcaaacg attaacggcg
     2461 tgcgcagcta tgccggtccg ctgggcaaaa gcgacagagc gctggtgttt ggtgcatggg
     2521 ggctgtcact gtcgatctgg ccgcaaattg tcgcctggaa taatgtcata tggggtgtcg
     2581 caacactgtt gttgttatgg acgattttta atcgctgtcg cagcgcattg cgtgaagagg
     2641 gtgtgaaatg acgctacttg aaaaatcact ggtcgttatt ttttccctgc tgttgattgc
     2701 caccgtaatc aacgggttgc tggtgtggct gcgtccaggt aaagactggc gcgagctgac
     2761 attacgtatt cgcacctggt gggtgattat cgtcctgttt tccttcgcac tattaagtcc
     2821 tagctggctg gcactgacgt tcttcgcgct ggtcagcttt atggcgttaa aagagttttt
     2881 gacgctggtg ccttcccgcc agtccgaccg catgccgctg ttatggatgt ttattgcaat
     2941 tccccttaac tactggctga taggtatcgg ctggtacggc atgtttgtgg tgtttatccc
     3001 ggtctacgtg ttcctttttt taccggcacg aatggtgatt gctggggata cacagggctt
     3061 tttacgtacc gtttcccagt tgcactggag tctgatgacc accgtatttg ccttcagcca
     3121 tgtcgcgttt ttactggtgt taccggcgga tggaatgcaa accagcgcgc tgttggtgct
     3181 gtttttggtc ggcctgaccg aatttaacga catcgcgcaa tacctgtggg gcaagtcgct
     3241 tggtcgtata aaagtcatac cgaaggtcag cccaaacaaa accctggagg gattgctggg
     3301 tggggtggca acgacggcgc tggcggcaac agtcctgggg ccactgttga ctcccctgag
     3361 ctggccgatg gcactgttag ccggggttat tatcggcgtt actggctttt gcggcgacgt
     3421 ggtgatgtcg gcaatcaagc gtgatatcgg cgtcaaagac agtggtacgc tgctacccgg
     3481 ccatggaggc atccttgatc gactggattc gctgattttt accgcaccgg tcttcttcca
     3541 ttttgttcgc tatttctgct actaacctat gatgacacga ctactccgta ccctgtttac
     3601 gctgtgtatc gtctggccgg tcatctggct gtggcttggt ctgcgtgtcc gccaccgcga
     3661 gcgactgccg aagcacgggc cttcgataat cgtggcaaat cacaacagcc atatggatgt
     3721 tttcgccttg ctttcgcttt ttccgctgcg ttaccaggct tcagttcatc cggttgctgc
     3781 cgcggattat tttctgcggg ataaacgcat ggcttggttt gcgctgaaga tccttaacat
     3841 cattcctatt tcaagaaagg gcggttatac cgatccgctg acgcaatgtg aacaggcgct
     3901 gcgtgaaggt aaaacactga tcctgtttcc ggaagggacg cgcggtgagc caggcgtact
     3961 tgcgccattg aaatccggag tgtggcattt gagtcagcgc atgccggagg tgcccattat
     4021 ccccgttggg ttgcgcggta ctgagcgtgt gatggcgaaa ggtaatcgta tccctttgcc
     4081 gctgtttatt gatgtaaata ttggcgatgc gctaagtacg catccggaga agaaaacgtt
     4141 tatggacgac ctggagcaaa aactgacggc gcttttgcaa ccctcgcaag gaaatcatcc
     4201 ccatgactaa ttcccgcata cccgatgaac ggcagtttct gaccagtgat aatacagagc
     4261 tgttttatcg ccactggcca gcacaagaag ccagttcaac gccaaaagtg gtggtcctgt
     4321 ttcatcgtgg gcatgagcac tccgggcgtt tgcaacacat tgttgatgag ctggcgatgc
     4381 cggatacagc gttttatgcc atggatgccc gaggtcatgg tcgctcaccg ggtctgcgtg
     4441 gttacagtcc gtcgctcgtg cgttcggtgc aggatgttga cgagtttgtg cgtttcgccg
     4501 ccgcagatag ccaggccggg atgacggatg tggtcgtcgt ggcgcaaagc gtcggggcgg
     4561 ttctggcggc aacctgggcg catgattatg ccccggacat tcgtggcctg gtgttggcat
     4621 caccggcgtt taaggtcaaa ttgtatgtgc cgtttgctcg ccaggggctg gaattgatgt
     4681 atcgcctgcg cggtcttttc ttcgtcaact cgtatgtgaa aggcaagtat ctgacgcacg
     4741 atcccgagcg ggtggcgagc ttcaataagg atccgctgat tacccgacca attgctgtga
     4801 atattctgct tgatctctac aaaacgactg agcgagtcgt cagcgatgcc agcgccatta
     4861 cgctacctgt gcagttgctg gtctccgggg aagattacgt tgtgcaccgc cagccgcagc
     4921 ttgattttta tcagaacctg cgaaatccgc agaaagaact gcatgtactg ccgggatttt
     4981 atcatgacac cttaggcgaa aaagagcggc atctggcatt tgacaggatg cgcagtttta
     5041 ttgatacctt gtatgcccgt cccgtgcagc gttttgatta cagccaggaa gatcgctgga
     5101 gccccggcgc cgatacctgg cgcatactga gcggtggccc tgcgctgtat tcgctggacg
     5161 atatctctta ccgctgtttg cgctacggga tgaaagtgct gggcacgcag tccacggggg
     5221 tgcgtctggg atttgagact ggtttcgatt ccggcagtac cctagactac atctatcgca
     5281 accagcctca gggcagcacc tcattggggc gcctcattga taaaaactac ctgaatagcg
     5341 tgggctggaa ggggatccgc cagcgtaaaa ttcatctgca gatgttaatt cagcaggctg
     5401 ttgctcggtt aacagagcag ggtagcgcgg tccggatcgt ggatattgcc gccgggcatg
     5461 gacgctatgt gcttgatgcc ctgcaaggcg aaacggcggt aagcgagatc cttctgcgcg
     5521 attacagcga attaaacgtg gcgaaaggtc aggagatgat agcctctcgc ggtatggcgg
     5581 gccgtgcgcg atttgagcag ggggatgcct ttaatcgtaa tgaactggcg gcactggaac
     5641 cgcgtccgac gctggggatc gtgtccggtc tgtatgaact gttcccggaa aacgggccgg
     5701 tacgagattc gcttgccggg ctggctgcag cgattgaacc gggaggcatt ctgatctaca
     5761 cctgtcagcc ctggcaccct cagttaaaaa ccatcgcctg gtcactcacc agtcacaaaa
     5821 atggcaaagc ctgggtaatg cgcgtacgta ctcagggcga gatggacgcg ctggtccgtg
     5881 acgccggttt tgagaaatgc acgcaactaa ttgatgaaat gggcatcttc acggtctcta
     5941 tggcggtacg acgtcaacca tgacaacgcg gcgtgcggtg attattcagg gaatcggctg
     6001 gctaatggtg ctggccccgt tcttctttct tacttatggc atgacgaatc agtttaccgc
     6061 cgggagagaa aacatcagca gcctggtgtt tgactgggag caccggatcc cgttaatacc
     6121 gctgacgatt attccgtact ggagtctgga tttgctttac tgtttatctg cgtttcactg
     6181 aatgagcagc ggcgtctggt ctgtcggctg gtgttggctt cccttattag tacaataatt
     6241 atctatatcc aaactggaca gataaaaagt tcccgtgttg tttttaagag cagcaaaatt
     6301 catggtcggc gaatatcccg gaatacagtc acactgtaac catgcatcat actcacgcat
     6361 ttcccggaga agatcattgg ccagagcatg tagtgccgta gttgctgttt tttcagtccg
     6421 cagaccgcct ttactgccat ctcttgctca cagctaagct cccgaatcac tttgctccgg
     6481 ggattcttct ttacaagctt cattggcata atgaatgcac tccttatata catacttaaa
     6541 cagcaagtaa aatatacaaa gaacatccca tgaagcaaag gcagagatag actgtgcgcc
     6601 ccgatatatt cttctgatat tttgctttat ttacttcaaa ttaatttact tctgtcagaa
     6661 ccacaacaaa gtggatacaa tatatttttt agaggc
//
LOCUS       DAZGTJ010000164         6668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000164 DAZGTJ010000000
VERSION     DAZGTJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.164"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            705..875
                     /locus_tag="ACHK8E_004870"
                     /pseudo
     CDS             705..875
                     /locus_tag="ACHK8E_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1091..1924)
                     /locus_tag="ACHK8E_004871"
     CDS             complement(1091..1924)
                     /locus_tag="ACHK8E_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0347012.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1931..3268)
                     /locus_tag="ACHK8E_004872"
     CDS             complement(1931..3268)
                     /locus_tag="ACHK8E_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFP0347013.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(3366..4376)
                     /gene="iolG1"
                     /locus_tag="ACHK8E_004873"
     CDS             complement(3366..4376)
                     /gene="iolG1"
                     /locus_tag="ACHK8E_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFP0347014.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4394..5299)
                     /gene="iolE"
                     /locus_tag="ACHK8E_004874"
     CDS             complement(4394..5299)
                     /gene="iolE"
                     /locus_tag="ACHK8E_004874"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFP0347015.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
     gene            complement(5787..6604)
                     /gene="reiD"
                     /locus_tag="ACHK8E_004875"
                     /pseudo
     CDS             complement(5787..6604)
                     /gene="reiD"
                     /locus_tag="ACHK8E_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
BASE COUNT         1955 a         1610 c         1245 g         1858 t
ORIGIN      
        1 gcttaccttt aactggttgc tgatataagt ttttcaccac gcataaacac tgcccatgcg
       61 atcctggcaa tcttatttgc cagctctatg atgacgatat tcgttggtcg acgtgcctgc
      121 tgggcttaaa tctattcccc cggggatccg gctttattgc aaccataaag ctgcgagccc
      181 ctatgtaatt cgctgactca cacctcattt actgcgcggg attcaagccc aaaaccccat
      241 ttaggtttag tgagtccctg tcttacctgt ctttgtcatc acgttgacca ccagagcaac
      301 ctttctgcat tcacccattt aagtaaccaa aatgagtatc aggcctgata tatacagaac
      361 tgaattcgtt aattaattat taatcagtta aattttccgt tactgggatg aggtgtacac
      421 ttggcctctc ccaaaatccg cggaaccgcc cctggcccct attcagcatt tcaggctcta
      481 agttgaggac tgaccggatt gacatagggc ctgctgacgc cattcattga ctaccaacag
      541 atctttcttg ttatttatag aaaaccacca gccagcctag tggttcagta aaacttccaa
      601 ctttgaggct gttgtcaaaa cccctttttg atttgttaaa acaccttgca gtctaccaac
      661 tgtaagcgtc aaacaagaaa tcaaaagggg attccaatgg gtgaatgaaa aagggcttaa
      721 cgaacacccg atggaactgt aaatatcaca tagtttttgc gccaaaatac cgaagacaga
      781 tgttctacag agaaaagcga agtgcaagag caccaggcaa tattttgaga aaactgtgtg
      841 agtggaaaag tgtacggatt ctggaagctg aatgctgagc ggatcatatc catatgcttg
      901 tgaatttacg gggcgtaagt aaggaagttg aatgaaaatg tcagatcata tgcgcctgtc
      961 agccagggcg aggcagaaaa cgaatcttta agacacatct gaaaaacctc cggctatgcc
     1021 ggaggagatt tattctgatc acctgcttat taccgggcac agcgacagtt catttgcagt
     1081 tacgtcagcg ttaatccgct aagaaaggtc gcgttttttc cgctccgata gcaaagacct
     1141 cgctggctgc catttgccag taacctgggt taaacaactc cagtgaacag atccctgtat
     1201 aacctttttt caccagttca tgggtgatca tgtacagtgg tatcgcaccg ttgcctggaa
     1261 acagacgatg acaatgatcc aacacagcta caggtaaacg gtcagcatca tcaatatgat
     1321 agacaaagat cttttccacc ggaacctgtc gcagcgtact cagatcctgc cattgatcgt
     1381 acagataaag gttaaaggca tctattgcca gaccaacgtt actccgatca actgcgtcca
     1441 cgatctccat cgccatcgcc agactgcgta cgcaacatcc cgcagagccg ataggctcaa
     1501 aggccagtcg catctggctc tctgccgcta atgctgacat ctcacgcaaa cgtttcactg
     1561 aatctcttac cgtgtcacga tctgaaaaat gagcaccttc acgaatggtc ggcactacaa
     1621 ccaggcaatc accacctacg acttcccctg cataacaggc taaaaccagt tgttgccgta
     1681 tttccgacca gcgcccggca tcacaaaatg tgatgtcttc cagcgagttg tagccccatg
     1741 gcttaagatg gcttacagaa aaaatatcgg ccagttcaac cagagtgtgt tcctgcagcc
     1801 acgtctgcaa catatccaga cggatctcaa tatagtcata tccaaaacgt tccgctagga
     1861 ccagatcctt ctccaggctg gaattacgca gacaggtcgc ttcattgaat cccagtttca
     1921 tcattcagac ttactcctgc cgccatacca atgtactggc tccagaaggc ggcagcgtta
     1981 atgcgagttc tctgtcgcca tcgaccaccc ggaaatgcct ttctcgcgta tcgcgattat
     2041 ataccactaa aactcgctca ccatcaggat taacaaaacc cacatcttcc agttgcgtat
     2101 cataactgga agtaagcata actttcgcac cgggatgcac aaagcggcaa aagtgtccaa
     2161 ttccgtacca agagtggtta caccagagaa tgtcattcaa tgcatcgtac tgcacaggag
     2221 cttcgcacaa attactctgg tgatttgggc ctccatcact gtctaacagc aggttccagt
     2281 cgataaatcc gctacagccg gatttaaaat taccaatcat gtcatgaata taggtatgcc
     2341 agtgacggat ctggccacct tcatcattct ccattggtac acatccttcg gaaaacagca
     2401 gctttttatt cggcaacttc cgggccagat actgaatttg cgaaaaatgg tcaccggtat
     2461 accagtggaa agccatcccg ttgatgctat cgccatcctt ttcgtcagca aaggcacgtt
     2521 cagcccagtc aatcagacca tctttatcat gatcccagat atagatctct atttcattca
     2581 ttccatagcg ggcgagtcgt gggcgcagat agtgaacagc aaaagtagtc tcttcttcta
     2641 cactatacag acaggaatcc cagcttttta tcgcaactgg ttcattctgc actgatagtg
     2701 ctttaacact gataccatgg cgtctgtatt cgagcagata gtcgataata atatctgccc
     2761 agtcagcata gtattcatac cgcaatttcc cacctccgtt catatcataa ttagtcttca
     2821 tgaatgccgg tggactccac ggggaagcca ttaattttat ttgtggattg atccgcaatg
     2881 ccgcggaaat tagcggcatt aaatgagatt catcacggga aaaggaaatc cttccctgac
     2941 gcaggtcttc actggaatca acataggcat aattgctcag agagaaatca cagctttgga
     3001 tcggcatccg ccccaaacta tagttatgtt cctgaggcga aaaatagaga ttcaaaaatt
     3061 gcgcctttgt cttatcactc atgctgttga aaacaacacc agcactttca gtgaagctgc
     3121 cgccaaatcc atcaatctgt tgtaacgagt ggtttggaaa gacgctgatt aattcactac
     3181 acgctggttg acgaggcgaa aaaaaaacct ccagctcaac cataaattgc tgattccagg
     3241 gatcggaagt gattagtctg cctttcatag agggattcca gataatatag gatgataaat
     3301 tgtgatatac cggacaggag catacaccta gctatccggt atgaaattat cacatcgcag
     3361 aaagactatt tgtagaagtc aggttttgct ggtaattcca caaactcggt gtttcctgtt
     3421 tcctgggact tcacacaggc atcagccgtt accgcagcca gataaccatc ccatgatgtt
     3481 gggcctgcca gaggtaatcc cgcattcaga cggtcaaaga agtcctggaa ctcaatatca
     3541 taggcatcaa taaagcgttg tttccagtcc accaaaatgt cagtactgta ttttgcctct
     3601 ttacgcaccg cagcagtagc cacggtaggt aattccacca tccctttctc acaggtcaca
     3661 tcacaatgaa tatcatagcc atactggcaa ttcacaaaca cctcaaccac tatattaata
     3721 ccggaggtcg tttccatcac caccacctgc ggatctcgta gagtagtcac caagctggac
     3781 tgacgtggga agtagacctt aactgttcta taatcttcgt tgagtaacca gtgcataaca
     3841 tcgatttcat gaatcagcgt ttcataaatt gcttgtggtg ttttgtaatc cgataccgtg
     3901 cttacattat aatgacgacc atgaaccatt aatggttgac caatttctcc gctatcgatg
     3961 atgtttttca gctgtacgta gcctttatcg tagcgacgca taaacccaac ttgcaccatt
     4021 cgttttccat ttttacgttc agctccgatc acacgctgac aatcatctgc tgtaaccgca
     4081 agcggctttt cacagaaaac atatttattg gcatttaacg ctgcaacggc tacatcggca
     4141 tgtacttcat tcgaggcagt aattaccact acttcgacat cttcatcatt aatcagatcg
     4201 tgatagtctt tatagtcttt tgctttgatc tcatacttat ccaaagctgc ttgcgctctg
     4261 ccttcgacaa tatcgcacac agcaacaact tctactccag aaatagtgtt agccagacgt
     4321 cttaagtgat cggcaccaat tttcccgatc cccacaatac ctgcttttaa agtcatctta
     4381 ttttcctttt ctatcagata taattaccca ataaaccatc aatatgcttt ttaccttttt
     4441 gtgcatattc caaaggatta tatatattcg ggtcctgctc agcttcaacg acaatccagc
     4501 cggaataatg attctcaacc aaataagcca atacagattt aaaatcaata ttcccatcac
     4561 caggaacagt aaacattcca tcaagaaaag aattgagaaa tgattttttt ccagcccgac
     4621 atgcttttag tttttcatcc cgaacatctt taaagtgaac atgtttaata cgggaaaaat
     4681 gcttgcacaa cagtgacatc acatctccat cagaaacata aatatgacca gtatcaaaca
     4741 gcaaattcac taattgtgga tctgtgtttt ccataagtcg atccacttcc tgtaaagttt
     4801 gtacgccagt gcccatatga tggtggaaag caagtttaag cccatatgta ttggcgatca
     4861 tgcctaactc gttcaatcct gagcataatt gttgccattc gatatcattg aagtaaggtt
     4921 tttccgtata gacacattta tcaataattc cttgaatact atacgtttgc tcagacacaa
     4981 cagcgatata agcatggaca gcctgcaaat aagcgcaatg ttgttcaaaa gctttcttca
     5041 ctgattcaat accatcacga ataataaaag atgagaacca ctttccggga atttccagat
     5101 ggcgaagtgc taactctata tttaattcag tcgggtccag aggataacat ccccccactt
     5161 ctgttccaga aaaaccagta agtgctgcat cacttaatat ctgcttatag gtattgtctt
     5221 taccaatttc aggcaaatca tcatttcgcc atccgattgg cgcaatcccc catttaatac
     5281 tctcttttac actatacata ttccagactc ctgcttaata aaactttctc acatcctgtt
     5341 gcataagtaa taatctttgg ctgacaatat gctactttcc acgtggctaa aaaactttat
     5401 ctatcatgag tacagaagtg ggaaaaatgt gtcaggaatc acaaaaaaaa cacaaccctt
     5461 taatgatttt gtctatgttt tagtcatgct gaaaaatcac acaatgaccg taaaataaaa
     5521 ccgtcacttt cccactaact ctggcgtgga cagaatcatc ccgtatgatt cccaagtttg
     5581 gtaaatatta ttaaatcagg atgtgcaagc tatgattcag tcatactagg aaaaaagttt
     5641 ttttgcagat tcggttggta tcaatgaaga cgatatataa taatatgcag gataggaaaa
     5701 gttcagcatc cttttagatg ttatacaaaa tccacctgct acgcaggtga attatttact
     5761 atgcgttaat ttatgttgca ttaatattaa aaaaataagc aattagcttt acgataatct
     5821 ttacgatatt gtccaggcgt tttcctactt tatttttaaa atagttctga aaatgactca
     5881 aattactgaa accaacacga taggcaattg aggtaatttt ttcacaccca ccttcatcag
     5941 tggtaagtaa agattgtgcc tcccctaacc gacgtttaat caaatagtcc attggcgaat
     6001 atccatagcg ccgtttaaat tcatgagaaa catagtaagg gctgacatta aacttagtcg
     6061 ccagagcatc cagacggatg ggttcataga aattatggtc aatataagct tttattgaat
     6121 ccagtaaatt tttatggcgc cgtgttatat cattacctgt gcgcattaat acatttccct
     6181 cgacgtattt aagcagcacc ctggtcagca actgcgccat tgttggtcct tctactgtat
     6241 gctgatttat aacattaaat attgaactca tcaatttctc gatcgtcgca taatgatgat
     6301 atagtggaat tacgggcaca gtatcatctg gaatcagagc attctcccgc agacccgggc
     6361 acacaatatt gtttatccca cagcaaatag tacccacagg ccgtccgctt tcaagatact
     6421 catcatgcac tacttcgctg ttataaataa ccaaatcacc ttttctgact gggtaaatgc
     6481 gatcaccgat gaaatactcc ccctttccct cggtgataag gattagctcc acggtcctgg
     6541 gatgcatatg catgaaccgt acaaacgggc cattgctttc gctaacctga ctgacatacg
     6601 ccaactgagc ccgggactta ttatcttgtt ctgaaggaca gaagcaaaaa cgcatgattt
     6661 ttcctcca
//
LOCUS       DAZGTJ010000165         6502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000165 DAZGTJ010000000
VERSION     DAZGTJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.165"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            54..1256
                     /locus_tag="ACHK8E_004876"
     CDS             54..1256
                     /locus_tag="ACHK8E_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP0347016.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
     gene            1491..3236
                     /locus_tag="ACHK8E_004877"
     CDS             1491..3236
                     /locus_tag="ACHK8E_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0347017.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            3302..3607
                     /locus_tag="ACHK8E_004878"
     CDS             3302..3607
                     /locus_tag="ACHK8E_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347018.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            3662..3976
                     /locus_tag="ACHK8E_004879"
                     /pseudo
     CDS             3662..3976
                     /locus_tag="ACHK8E_004879"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            4301..4450
                     /locus_tag="ACHK8E_004880"
                     /pseudo
     CDS             4301..4450
                     /locus_tag="ACHK8E_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4625..5059
                     /locus_tag="ACHK8E_004881"
                     /pseudo
     CDS             4625..5059
                     /locus_tag="ACHK8E_004881"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            5202..5291
                     /locus_tag="ACHK8E_004882"
                     /pseudo
     CDS             5202..5291
                     /locus_tag="ACHK8E_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5696..>6502
                     /locus_tag="ACHK8E_004883"
     CDS             5696..>6502
                     /locus_tag="ACHK8E_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HFP0347019.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWL
                     RLTHEQIKVIELLSEYQRVVVTGWPGTGKTLIGIEFARRLVKGQKKVLFIT"
BASE COUNT         1755 a         1381 c         1735 g         1631 t
ORIGIN      
        1 taaaggaaag tcatggcagg aagaaaaaag atgataatgc agcgggattt gtcctgattt
       61 tagtttgcgt tattctctgg gggatttatg tggcggtcag ggcgctgata aatcttaatg
      121 agcgctttat tgatgcagta tcgagtccgg caggtattat tggtttgttt tttggtttgc
      181 ttactgtatt tgcaatttta cttcgttttt ttatttaccg gagactcaga aagaaaacag
      241 cagcctttga gcaggccgtt tcagagctgg ttcagagaga gcggggtttt aatgaaactg
      301 tcaatgccgt gatagcccgg ggaatacgac aggaaaagga gcaactggcc agacgtcgtg
      361 aggaatttca taccgccaga cagaaggcca gccgtgcaat gcaacggatt gttgattccg
      421 cctggaaatt taaggcgaag actctgctgg ctggtgtaac gattaataac tggcaaagca
      481 aatatgacca gcttcgtaag gaaagagagg cttacgctgc tgtcagtgag aaaatagcct
      541 ttcttaacct ggaagataat tctgactggg agagtgtcag gcaacagttt ctggacaaag
      601 tggcattgct ggaaaaagcg caggaagaga aggaatatca ggcagagctt aagcgtcaga
      661 tacgtgaaga aaaagagcgt caggatgaac ttgaccggcg ccagcgggaa gcggaagagg
      721 aagaacggcg tcttgctgag cagcagaaac tgattgagga ggcgctgcgc gcagcagaag
      781 gggctcacag ggaggagtta gagaaacaac gcctggagct ggaacagaaa attcaggaag
      841 cacatgcgca gtatgaacgt gctaaatcca tggctcagct tacgaaacag gggcatgttt
      901 atatcatttc gaatattggt tcgtttggtg aggatgtctt taaaattggc atgacccgac
      961 ggctggaacc catggacagg gtcaaagagt taagtggtgc ttctgttccg tttgattttg
     1021 atgttcacgc catgatttcc tgtgatgatg cgcctgcgct ggaaaaaaca cttcatgaca
     1081 gtctggaaaa ataccgcatt aacaggatta acctgcgtaa agaatttttc cgcgtgaagc
     1141 ttgagaaaat cattaatgaa gtggagcgtc accacgggca gatggaatat gtggctgacc
     1201 cggcggcgct gcagtacctg cagagtctgg aatacgcaga aaatgaagca gcctgacagt
     1261 cagtgttctg attttacccc ggccggatat aacagaaggg atatctgcag ataccctgca
     1321 gcatggttca cagtattctg gtcatgttaa tgattctgtt ttttatttct gaataaagcg
     1381 acacccacga tataacatcc gtggatgtcg gtggtgatta ttttttgttc acagattaag
     1441 gtgcgtttat ggcgaaactg ataaatgtat cggcctccac tgaggagaga atgacgcctg
     1501 gggagaggag ggtggcttca cgactggaga gctttctgaa tgatgactgc cttgtctggt
     1561 acgatattcc ggtgggacgg aaaaaccggc atccggattt tgtcattatt gacccggaga
     1621 acggactggt atttctggag gtcaaggact ggacggtttc cacgctgcgc caggtaaatc
     1681 aggagcaggt cacgctggaa accgacggtc ttctgaaatc agaaatcaat ccgctggtac
     1741 aggttcgtcg ttatgcctgt gatacggtta atgccttacc tgcaaacccg tgcttacggc
     1801 agaatgacgg tcagtataag ggacggctga acctggcctg ggcgtatggt gtggtgttta
     1861 cccgcattac ccgtcagcaa ctgaagacac tggcagggaa tgatgagaat gcggtggaaa
     1921 aaatttttcc ttcagcacag acaatctgcc aggatgaaat gacgcagtct gttttacctg
     1981 aggtattcca gcaaaaaatt gccgggatgt ttaccacggg attcagaacc cgggtgacac
     2041 cccggatgag agatattctg cgcgcccatc tgtttcctga agtgaccgtg aaacagaact
     2101 cacaaatcaa aatcatggat attcagcagg agattctggc cagaaatatt ggtgacgggc
     2161 atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat gattcttttg ttccgttgcc
     2221 tttacctggc tgaaaccaca cccggaaaga ttctggtgtt gtgctacaac attaccctgg
     2281 ccagctatct gcgggaatgc attgaagcca gagggttggc gtcacgggtt acggtatccc
     2341 attttcacag ctggtgtgcc tcaatggtaa aacgccacgg tattaaggtg acggccgaca
     2401 gaaaagaata tcctgataaa tgcttcagtg cactggaaga ggccgtgaac agcggaaaaa
     2461 tcacccgaac cggatatgat gctgtactgg tggatgaggg gcatgatttt gacagtcgct
     2521 ggctggccct gattgcccgt ctgtttgata acgcatcccg ttcactgctc ctgatgtacg
     2581 atgacgccca gtccatttac cggcgggagc gggccctgaa cttttctctg gccagtgtgg
     2641 ggattcaggc acagggacgc acttctgtcc tgccggtcaa ttatcgtaat ccgaaacgca
     2701 ttctgcactt tgcttatgca ttctcgcgcg agtattttga aaaacatcag aaccgtgaaa
     2761 taccgctcgt tcagccacag gcgggcggtg aagagggtac ggagccggaa atcctgcgtt
     2821 gtgcttctga acgtgatgaa gccagacaag ttgcagactg gctggagaag cgccatgccc
     2881 tgtgtggtca ctggagtgat atggctgtgc tgtgtccgac agagcgttca gcgcaacaat
     2941 tgcaggacgt tatgacacta cgcggggtgc cttttgccac ctgttttgac agggaaggca
     3001 agaagacata ctcgcgacgt aaggatgtgg tgcatttact gatgtaccag agcagcaagg
     3061 ggctggagtt cccgtatgtg gcggttatca atgcgtcttt cgttcactct ggtgcagcgg
     3121 atgagtctga agtgattccg gtgctttatg tggcgttcac ccgggcaacc cgtgaattac
     3181 tggtgacctg ttaccgtgaa aacagtatca gtcgtcacct tgaggatttt gcataatacc
     3241 ggattatttc tttattccgg actttcatca tactgttatt atcaggttta tttaattcgc
     3301 catgaacccg ttatttaatg atattcagat gcgtcttttt tatctgaacc actctccgta
     3361 ctcctggcac tggaatgtca gattcagacc ccaggaagct gtctatattg gcagcgatac
     3421 ctgtcatatc accattacct gtaatcagtc cggttttcat ttaaccaggg acggacaacg
     3481 gctgttcact gaacgttata tcagaaacct taatgagctg ttaccggttc tgaaacggcg
     3541 gtgggatgtg acaccggcaa tcatccgtgc cgtggaatac ctttcccggg tcccggtatt
     3601 acactgacat tcttttcgtc actgttaatc atgctttaat acagtaacac cgaatctgcc
     3661 gatgacttct gaagtgaaac tgaaaaccgg tggtgacccc aggtcattac cggattatgc
     3721 tgccctgcgt gatgaaatca gcaaactgac ccacccggca cgtccggatg tggactggcg
     3781 ttacgtggaa acgctctgcc tgcgtctgta tgaacacaat ggcgtggaat tacagacggc
     3841 atcctggtac accatcgccc ggatgcatac agcagggtta agtggactga atgaagggct
     3901 ggctctgatt gtggcgctga cccggcatca ctggtcagtg atgtggccgc tgaataccca
     3961 cgcccggctg gaaattatta ccggtctgtt caaccgtctg caaaaaacgc ttcgggccat
     4021 gcctccggac gaccgtgaca acctgcccct gctgtatcag accgaaacat tcctgaaggc
     4081 actgagtgac acacttgccc ggcacgaact taaacagggc agtaaagtgg ctttaccgga
     4141 agccatggtg aagggatata tcacccgcct ggaaaatcag cctgtgcagg gcgaagcgtc
     4201 gtctccggta acgcttccgg ctcaggcact ccggtctgat gctccggatg tgcaggaaca
     4261 tcagaccctg ccgcactccc gtctggtgta tgttgtttct gaaacaaaag cagagcccac
     4321 accctccctt cagaaatccc cgcccatgtt tctgaagccg tttttggctg gtgtctgtgc
     4381 ggcattgctg gctgtcagcg tggccattcc gggatggcag tttctgacac agccatcacc
     4441 ggcggagcag caactcaggg cgctgctgac acccccaccg gatgtgcttt cggcagacca
     4501 gatagcacag ctcagcagaa cacgctcact gttaaatcat acgtcagagt ggatacccct
     4561 ttccggtcaa cagataacgc gactgacaga attacccctg gcatacaacc tgcagcgcag
     4621 tgcgcagctg ctgcagcaac tgagggtact tttccccgat aatccccggg tacaggaaat
     4681 ggtggataac tggcagaaga gtgtaaggag ccgtgcgctc ccggaagagg cgatgacggg
     4741 ctggaacgaa ggcatgaccc gcctacagca gctggctgag cgcttgaacc gtctggatga
     4801 acagcgggga aaatacatga cagtcagtga actgaaaaca gaggtgtttg gcatcatgca
     4861 ggcttttaac cggcatatcc cggcagaaga gcagttacgt cgctataatg aagtccgcaa
     4921 ccagaatggc agtgaacaac agcaaaaact gacgcaggat gccctgacgg aactgttgaa
     4981 ccgctactgg tttctgagac atggagatgc aggtaagaag acgataaata ataaacagga
     5041 taaccagatt aatatctgac agattttatt gccattattt atccagaagc gatgatgcca
     5101 ttaatgcaaa atgacatctg aattgatcac agattggacc aatctgtgat caaatagaac
     5161 agaatacgca gatgataaag cggctttccc tcgaagattc acaacgtcat ctggcagaat
     5221 atgggtgaga gttgcaggct gatgcgtaca aagcttacga tgcgttgtat gaaactggcc
     5281 gggtgaaaga tgtcatccgt acggtgcgcg ccagactaaa caggcatcat gatggaaaag
     5341 tcgggtatca ttcacaggca tatttgtcag aatggtagcg tcagcgggtt ttgattacac
     5401 cagttttgaa gctcatactt cctatgaggc actgcagaaa acgaaagtgt cgtttaggcg
     5461 ctgggggagg ggttaaaaaa gtaacggcag aagtcaccct gaagttgagg cagtcaacta
     5521 ctgttagctg gctgctgcat gagagtgtgg agggagattg tatatattgg ttagtcagag
     5581 tctgcttcgg aataagtatg agtaattttt aaaatgggcg gaagttattt ttaattactg
     5641 ggctatgtaa caaatgcaga ttcctgtcag aaacatgtta taaggaaata gtaatatggc
     5701 caaaatgatt ccggcatttg gacccattga taataatagt tatggggaaa aaacagtcta
     5761 tacactgtta aaagaaggtc tgtccgatga tttcacagtg attcatagtc tcccgtggtt
     5821 aagtgcggcg acacgaaatt tagggatgaa attacctccg agtggagaaa ttgattttct
     5881 tgttctccat cctgtatatg gtgttttggc gttagaggta aagagtggaa tatatcgcgt
     5941 agaaggtact gtttttgtac atataaaaac aaaaaaacat gtagatatag tgaagcaaac
     6001 acgtaacaat gtccatggtt tagcccgttg gttaggggga gcaacaacgt tacggttgcg
     6061 tattggttat ggttttattt ttcctgatag ttatttcgat gataaaatta taaatgcagc
     6121 aatggtagat gcgtctgtta aaccattcag gggaatattt gttgataaaa gccaactccc
     6181 ggaaatggca aagcatacta ttgatataat gcaatactgg aaacatgcat tgggaaataa
     6241 tgagttgggg gatgaacggg ttcaactaat aataaaatct gtatgtcctg agtttgatgg
     6301 tactcctgac tggggaatcc gtattattta tgataataaa ttatggttaa ggctcacaca
     6361 tgaacaaatc aaggtcattg aacttctgtc agaatatcaa agagttgttg taaccgggtg
     6421 gccaggaaca ggaaagaccc tgattggaat tgagtttgca cggcggcttg ttaaaggcca
     6481 aaaaaaggtt ttatttatta ct
//
LOCUS       DAZGTJ010000166         6172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000166 DAZGTJ010000000
VERSION     DAZGTJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.166"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            94..1645
                     /locus_tag="ACHK8E_004884"
                     /pseudo
     CDS             94..1645
                     /locus_tag="ACHK8E_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
     gene            1671..2123
                     /locus_tag="ACHK8E_004885"
     CDS             1671..2123
                     /locus_tag="ACHK8E_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP0347020.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            2276..3550
                     /locus_tag="ACHK8E_004886"
     CDS             2276..3550
                     /locus_tag="ACHK8E_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0347021.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3543..5644
                     /gene="mchF"
                     /locus_tag="ACHK8E_004887"
                     /pseudo
     CDS             3543..5644
                     /gene="mchF"
                     /locus_tag="ACHK8E_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
     gene            complement(5705..6028)
                     /locus_tag="ACHK8E_004888"
     CDS             complement(5705..6028)
                     /locus_tag="ACHK8E_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347022.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPSADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT         1789 a         1165 c         1467 g         1751 t
ORIGIN      
        1 agttttactg taataaggaa gaagctgtca ggacacttac ttgctctttt atgaacactc
       61 ttttatgaaa attgaaacgg agaatactgc cagatgagtc atcagtgttc actttctgaa
      121 ctgaatgaaa acctggtgcc tttcactgcc aggcagatca aggcctcatt aatctggtgt
      181 gcagaggatg tcagaaatcc agacgagctg caaaatgcct gcagttatat tatcgatcct
      241 ggcagtacag cttctgccaa agtgttccat gcagagcgct atggtggcag tggtattcag
      301 cgtaatggag gtggtgcacg ttgtgggttt gatggtaact accaggttaa aggaatagga
      361 agtaatccgt tggttggcga aggtactgac gaacgtcatt ctaatggtgc actcggcgct
      421 gttcatgcaa tattatgagg ctttgtgggg agaagtactg gctcaaatat taccttatag
      481 tgctgtgcgg gttcgggcgg ttttacttac agatctctat actgaaaagg catttgagcg
      541 ctccggtagg aaatcacgaa gagccctgtt ggtacgtgag cctgttgttc gtccggcgca
      601 ttttgaacgg gcaccttact tccaagtaaa accggagtat tccagtctgc taattcacga
      661 tgcctgtcgg gttagatctg tgatccacaa gctgccagga tatctacctg taccaccgga
      721 agaaattgat gctgaagcac gaactgatcc ccggatttat tgcattgagg ggttatgtga
      781 actggcacgt cgtgaggcct ggcaaatggc attttgtcga acacgtttcc tgagattgac
      841 aacttctcct tctaatattg caatggatgg cagattaatg gattttaacg gactcagttg
      901 tctgtttccg ggagattccc cagctgattt tgggtataaa ctaagattag ctgaactggc
      961 aaaagaaccg atggtactta tgcaagggct gtctgatctc tgcttgtata tcggaaaata
     1021 tatgtttgac cctgacttca ctcttgcagc ccgtttgaag gttgaggaga tatttcagaa
     1081 aactttccat gaagcatgtt attactgtta tctagaactg ttgggtattc ctggagaatt
     1141 tataacacaa aaagagatac ctgatatatt gaaagaactg gttaacagtt ttgttgcatt
     1201 actcaatcaa tactgcgaga aatcacatgc ccaagatatt gtcaatcagg atggttcacc
     1261 attgcaaaag ttggttgtga cgctaatcca tcataggcat aatcaaaagc aggcactgaa
     1321 tagtagcatc aagaatgatg tttatttcac cgttgcacaa cagtgttttt cccaagccat
     1381 ccactggctg acgcaaggca gtaccagacg tcagataaat gcctcattac tcctgaaaga
     1441 aattgaacat cataccatga aaaggctgca acccagggaa gagctgagga aagaaaatat
     1501 gtgcgaaaaa attgccatcc tgctggataa tcatggcgat gatccccttt ttttacaaga
     1561 agcaatttct gatatgaaaa attttatgct taagttttcc agagatgcat ttggatatct
     1621 tgaaccgata agaaacacag tgtaaaaaat atataaggga aatagtaatc atgtcttata
     1681 taagggaaac catcagagga aaagatgaat ggactgttta tgaacagatc ggttttgcgg
     1741 tcagttgtat gctctacaat cgtaattaca gtctgtatcc ggtgttaacc attcaatact
     1801 ggactgaata tgcgatacag cataatcaga ttaaattcct gtttgattca cgaggttttc
     1861 cactggcgta tattacctgg gcatatcttg aggctgatac agaagcgcgc ctgctcaggg
     1921 atccagaatt caggttgcat ccgtctgaat ggaatgaaga tggaaggatt tggattctgg
     1981 atttctgttg taaaccaggc tttggtcgaa aagttattga ctatctcata cagcttcagc
     2041 catggggaga aggagaagta cgatggttaa gcaggcgaaa gaaaattgtg acattcatcc
     2101 ctgagcggct gcataaaacg tagtacctct gaagatacag agataattgt aaattacggg
     2161 gtaaatgcat cgctgatact attttgacag gactctgtat tttctggctt tgattatagc
     2221 atttcgctta accagtgaag gtattacagg aaaagtgtga gtaagaggag ctattttgtt
     2281 tcgtcaggat gctttagaaa acagaaaaat gaagtggcag ggacgggcaa tattacttcc
     2341 cggaatacca ctatggttaa tcatgctggg aagcatagtg tttattacgg catttctgat
     2401 gttcattatt gttggtacct atagccgccg tgttaatgtc agtggtgagg tcacaacctg
     2461 gccaagagct gtcaatatat attcaggtgt acagggattt gttgtcaggc agtttgttca
     2521 tgaaggacag ttgataaaaa aaggggaccc tgtttatctg attgacatca gtaaaagtac
     2581 acgtagtggt attgtcactg ataatcatcg tcgggatata gaaaaccagc tggttcgtgt
     2641 ggacaacatt atttcccgtc tggaagaaag taaaaaaata acgctagata ccctggaaaa
     2701 acaacgcctg caatacacag atgcgtttcg tcgctcatca gatattatac agcgtgcaga
     2761 ggaagggata aaaataatga aaaacaatat ggagaattac agaaactatc aggcaaaagg
     2821 gctgattaat aaagatcagt taactaacca ggtggcatta tattatcagc aacaaaacaa
     2881 tcttctcagc ctgagcggac agaacgaaca gaatgctctg cagataacca ctctggagag
     2941 tcagattcag actcaggctg cagattttga taaccgtatc taccagatgg aactgcaacg
     3001 gtacgagtta cagaaagaac tggttaacac tgatgtggag ggcgaaatca ttatccgggc
     3061 gttgactgac gggaaagttg actccctgag tgtcactgtc gggcaaatgg tcaataccgg
     3121 agacagcctt ctgcaggtta ttcctgagaa cattgaaaac tattatctta ttctctgggt
     3181 cccgaatgat gctgttcctt atatttcggc tggtgacaaa gtgaatattc gttatgaagc
     3241 ctttccggca gaaaaatttg ggcagttctc tgctacggtt aaaactatat ccaggactcc
     3301 tgcgtcaaca caggaaatgt tgacctataa gggagcacca cagaatacgc cgggcgactc
     3361 tgttccctgg tataaagtca ttgcgatgcc tgaaaagcag attatcagat atgacgaaaa
     3421 atacctccct ctggaaaatg gaatgaaagc cgaaagtaca ctatttctgg aaaaaaggcg
     3481 tatttaccag tggatgcttt ctcctttcta tgacatgaaa cacagtgcaa caggaccgct
     3541 caatgactaa cgggaatttc agacaaatta taaatcagct tgatatgcgc tggcgacgtc
     3601 gtgttccggt tattcatcag acggagaccg ctgaatgtgg actggcctgc ctggcaatga
     3661 tatgcggtca ttttggtaag aatattgacc tgatatctct tcgccggaag tttaatctct
     3721 cggcccgtgg agcaaacctt gcaggaatca atggaatagc ggagcagctg gggatggtca
     3781 cccgggctct ttcactggag ctggatgaac ttggtgccct caaaatgccg tgtattctcc
     3841 actgggattt cagtcacttc gtcgtgctgg tcagcgtaaa gcgtaaccgt tatgtactgc
     3901 atgatccggc cagaggcaga agatatctcg gtcgggagga aatgagccgg tattttacgg
     3961 gcattgcact tgaggtctgg cccggaagtg aattcctggc ggaaacccag cagacccgca
     4021 taagtctccg ttcactgatt aacagtattt acggtattaa aagaacactg gcgaaaattt
     4081 tctgtctgtc agttgtaatt gaagcaatca atctggtaat gccggtggga actcagctgg
     4141 ttatggatca tgcgattccg gcgggggaca gagggctgct gacgcttatt tctgctggcc
     4201 tgatgttctt tatattgctc agggccgcgg tgagtatgct gcgtgcatgg tcctcactgg
     4261 ttatgagcac gctcatcaat atacagtggc agtcgggtct gtttaaccat cttctcagac
     4321 tgccgctggc gttttttgaa cgccgtaaat taggtgatat ccagtcgcgt tttggctccc
     4381 ttgacacttt gagggccacc tttaccacct gtgttgttgg ggcaatcatg gacagtatta
     4441 tggttgtggg tgtttttgtg atgatgctgt tatatggagg atatcttacc tggatagtgc
     4501 tcggttttac catggtttac gttcttattc gtctggtgac atacggctat taccggcaaa
     4561 tatcggaaga aactcttgtc agggggcccg ggccagctcc tattttatgg aaagcctgta
     4621 tggtattgcc acggtaaaaa tccagggtat ggctgggatt cggggaacac actggcttaa
     4681 cctgaaaata gatgcgatca attcaggtat taagttaacc aggatggatt tgctcttcgg
     4741 ggggataaat acttttgttg ccgcctgtga tcaggtggcg attttatggc tgggtgcaag
     4801 ccttgtgatc gataatcaga tgacaatagg aatgtttgtg gcatttggtt cttttcgtgg
     4861 gcagttttcg gatcgggttg cttcgctgac cagttttctt cttcaactga gaataatgag
     4921 tctgcataat gagctcattg cagatattgc actacatgaa aaggaagaaa agaaaccgga
     4981 aattgaaatc gttgctgaca tgagcccggt ttcactggaa accactgatt taagctaccg
     5041 gtatgacagc cagtcagcac aggtattcag tggtctgaat ttgtctgtgg ctccgggaga
     5101 aagtgtggct ataactggtg cctccggtgc cggaaaaacc acattaatga aagtattatg
     5161 tggactgttt gaaccagata gtggaaaagt actggttaat ggcacggata tacgtcaact
     5221 tggaataaat aattatcacc gtatgatagc ctgtgttatg caggacgacc gactattttc
     5281 aggatcaatt cgtgaaaata tctgtgggtt tgcagaagaa acagacgacg aatggatgac
     5341 agaatgtgcc aaagcaagtc atattcatga tgtgataatg aaaatgccaa tggggtatga
     5401 aacgttaata ggtgaactgg gggaaggtct ttccggcggt caaaaacagc gtatattcat
     5461 tgcccgagct ttataccgga aacctggaat attatttatg gatgaggcta caagttctct
     5521 tgatacagaa agtgaacgtt tcgtgaatgc tgctataaaa aagatgaata tcacccgggt
     5581 gattattgca catagagaga caactttaag aacagttgat cggattttta gaattacaaa
     5641 ttaggataat atatcactaa aagcagcatt gctgcataat aagctatgct gctttagaaa
     5701 ttaatcaact tccactacta gaagaggatg ttgatgagtt aaatcctgag tttaataaac
     5761 ctccattgta gtttggatta aaacttccaa gtcctatact cggattatag ttaataccgt
     5821 ttgatggtat tgttattgtc ctatttgaac catcagcaat aactcctcca atatatcctc
     5881 caattgcact tcctgctata tccgcactag ggatttttgt tgctttacct aaaaaaccac
     5941 cagccgcagt acctattgca acggatgcat ctttatctaa tcctgcacca tgtattaaag
     6001 caagttcatc ggttgttata tttctcatgt tatttccttt ttttattaaa gaaagataaa
     6061 acaaataaaa acagtacact cgcaagtaac gaaagaatta ttttctttat gaaaagataa
     6121 agggttaatg tgtctaagtc tgaatttata agttgtatta taaagaacaa cg
//
LOCUS       DAZGTJ010000167         6126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000167 DAZGTJ010000000
VERSION     DAZGTJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.167"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(224..1920)
                     /locus_tag="ACHK8E_004889"
                     /pseudo
     CDS             complement(224..1920)
                     /locus_tag="ACHK8E_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            complement(2016..3152)
                     /locus_tag="ACHK8E_004890"
     CDS             complement(2016..3152)
                     /locus_tag="ACHK8E_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0347023.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            3828..4036
                     /locus_tag="ACHK8E_004891"
                     /pseudo
     CDS             3828..4036
                     /locus_tag="ACHK8E_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            4023..4212
                     /gene="tnpB"
                     /locus_tag="ACHK8E_004892"
                     /pseudo
     CDS             4023..4212
                     /gene="tnpB"
                     /locus_tag="ACHK8E_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(4215..4403)
                     /locus_tag="ACHK8E_004893"
     CDS             complement(4215..4403)
                     /locus_tag="ACHK8E_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0347024.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            4938..5079
                     /locus_tag="ACHK8E_004894"
                     /pseudo
     CDS             4938..5079
                     /locus_tag="ACHK8E_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            5302..6039
                     /locus_tag="ACHK8E_004895"
     CDS             5302..6039
                     /locus_tag="ACHK8E_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0347025.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT         1688 a         1343 c         1210 g         1885 t
ORIGIN      
        1 atcgtctctg ataaatactg ttgagctata tctgtactct gtttttctaa atcagaagct
       61 ttctttgttt caagaacaga tttgattaac tccttacgtt gtgtacacca aatctcgtca
      121 gatgaacagt attttttatt ttcgtgtttg ttcattattt attgctctaa ctgatttatt
      181 tccctatatc cagggagtag atatatctac tccctgctta ttatcatttc acgttagtta
      241 aagatttttt tatattctca aatttggctt ggttgatatc actgatcggt ttttgacttg
      301 acgtgatgaa gtcaatcata acagccttca tattcttaac tacatcagga tgggatgcag
      361 agagatcttt agtttgttgc agatcgttga tatcgtacag agctagttta ttctctgcat
      421 aggtgtaaac cagagaataa tcgttgcttc ttacagtgta tgcaaactgg cttaagtctt
      481 ctgtatgtgg gttgtgcgga taatcatcag actcttttct cacgaattta tgatatccgt
      541 tccagaatgc agtgttctct tcgtcccacc agtgagaata agaggtaatc caggacagat
      601 atttgtgagg ttcgccagct gcagatttct tatcagctcc tttctgtaca tatggcaata
      661 atgatacacc atctagtttc agagaagcag ggatcttgat atctgcggca tctaaagcgg
      721 tcggatagaa gtccattgat gaaaccagtt tgtcataatc acccgttttc agcttgccat
      781 tccaccacat gaacataggt gtatgcatac caccggatac ggttggcctt tgtaaccagc
      841 ctgcgcacca ttcagtggca gtggaccatc aattacagca ccgttatcag aggtgaacag
      901 aatgatggtg ttgtcgtact gacctttttc cttaagtgta ttcaggatct ttttaacccc
      961 ctggtcaacg gagtaaattg acgcataata gttatcagca gtcttgctac cagtgttgaa
     1021 tttctcctga tagtttttag gcgcaggctg atcgttaggc aggtgtggtg cactgtatgc
     1081 cagataaatc ataaatggct gttccagttt gcttgcacgg tcaattacac caattgcttc
     1141 atcagttaat tgatcactaa tgtacccttc agctttaatg ttttcacggt tacggaacaa
     1201 tgacggggag ttataaagtg aaacgccgga tgcgtgataa cccatgaagt agttgaagcc
     1261 acgattctga ggttgccatt cttctgcgga gaaggtggtg aagttatcgt ggtagtcacg
     1321 ggtttgtttt tcttcaggca cacctacgtt gctgattttt gataagtgcc acttaccgat
     1381 tgctgcagtg taataaccat gattctgaag taattcagac aggaatgttt cttccagagg
     1441 gataccatcc tgtgcatcag aatttgaata tacaccgaag cgagcaggag ctcgcgccgt
     1501 caggattgcg gcacgtgaag ggccagagac accgtgtgca acatagccgt tagtgaattt
     1561 tacaccttcg tccatcaggg ccgctaatgt tggagtggat ttttttgcag cttcaattgc
     1621 tttatcaata cctattttgt atgtatcaac aacatttcgg ttatccatcg ttttagggtc
     1681 aaatgaacta tcatcaaaag gcaattgtcc ataccctaaa tcatccattg ttaaaacaat
     1741 gatatttggc ttacctttag tgctgtattc cgtcggcgtg aagtccgcaa aggcaacatt
     1801 ggtttttgtt gctttgagct taacatcatc tgctgcattt gcagtactca tcatcagacc
     1861 agatgctaat ataagtgata ttgatgagtt aagcagtttt ttatttaact gatgttccat
     1921 atggtattcc ttctgagttg catcacgaac atttattaat aataaaagcc tctgatataa
     1981 ataaaaacta tgtaaatcgt ttaaatggat acctgttaaa actgatacat cataccaaca
     2041 gcaagtatat tatcggttgc aactccagcc tttttggtaa attcgctttc atcaataagg
     2101 ttaattttgt aatcaataag aagtgacatg tttttgttca gaaagtagtt tgcgccaaca
     2161 tcaatatact caacaagaga ctgatcgcca tagtttacta ttccaccttt aacatccatc
     2221 cctttggact gaacgtaagc cagtgaagga cgcaggccga aatcaaacat atactgagcc
     2281 acaacttcaa tattccgtgc tttatctgcg atgcctgcgc taccaaatgg agtcatattt
     2341 cgggtttcag agtagtcagc tgccagatac aggtcattat cattatactt gaccccaaca
     2401 cctgcaaact ccgcatgttt tcccccggca atccagcgag gcgtaataac ttcttttcct
     2461 tctgaatctt tcgatgtcat gaaacgttta cggccttctt tttcctgcag actggttctg
     2521 tcggcattag tgtaagcggc aacgattcca aaaccttcat agtcatagct tacggagaaa
     2581 ccatatccat caccattagc ttcaagtaaa ttatcacggt catttttacc ctgatattga
     2641 gcagaaaaag taagtccatc aacaagcccg aagaaatctg tattgcgcca ggtcagaact
     2701 ccggttgttc tgccggtcat atagttatca gagtaagtcc agctgtcacc accaaagtcc
     2761 ggaagtacgt cagtcagagc ccccacatca taggcaacac catagtttcg cccataatca
     2821 agtgaaccga gtatattgtg gcgaatacct gcaaatgcca gacgattttt atcgttgcgt
     2881 acttcacttt ctgtcttaaa ccccaggaac tggtattccc actgaccaaa gccagtcagg
     2941 ctctcattaa tttgtgtttc gcctttgaaa ccaagacgtg caaatgttct gtcgccatcc
     3001 atagaaccat cagagatgta atgacgggct ttaacgcgac cataaaaatc cagtttatta
     3061 ccgtctttat tatagatttc tgctgcctgt gttgcctcac acagcattaa tgcagaaacg
     3121 acagagcaca caaccatact taattttcgc ataaaaaaat cccctgtgca catttttagt
     3181 atggttttaa aaattaaact atggcgaaaa aattatcact atgcaaagat gtaacaaaat
     3241 attgctcagt gatctatatt gttgttttta tttgaggttt tatattttct agtgtatttg
     3301 acttttcttt ctcaagtgtt atttcttgac ctttatcaca gaaccatctc cctggatatc
     3361 cccatcatat tgcatgaaaa gcaagtttta ccaatcttga ttttcagtgt taaataagat
     3421 taataactgt aattctgcaa aaaaaagaac accatatgta gtatgactac gtattccact
     3481 ctgttcgttc acggactggc atcgaagtct gaggtgtact ggcaatagcg gatacagtca
     3541 tttgttcttt ttttaagcat tcatccgatg atacttttcc tgaatgctac cggagtggta
     3601 ttcccctgcc gtgagggagg gcgctgacgg ttataaaaat cctgatatat acccattccc
     3661 gtatgacagc gatggcgtca catctctgtt ctaccatctt cacagaaata tttcgttcat
     3721 tagcattccg gtattcgtat aaccaccttc ggtggagaaa gaataaacaa tccctggaat
     3781 gcgatgagcg cttgaccaga aaagcatcat attgcttcat ggcgcatcag cgaccacagg
     3841 atgccgcaaa agccgaaaaa cggaccagag cgcctgcaat cattcctcgc ctcagtatct
     3901 gttgcccatg gagtggccga taacagatca ttgtcggatg agccttatcc tcttccttcc
     3961 cggtggtatc cggatgtgct gccagccttc tcagttaacc gatgttttcc gggctttttg
     4021 ccatactgag gactgataac gtattcctgg tcgtaaaacc cgtcaatatg cgccggggca
     4081 tcgacaaaac tgacacagta cgtccggaaa gaacttaacg ctgcatggca cgacaatgct
     4141 gcctttgtct ttaccaacaa ggctcgttcc cgcataaaag ttctacgccg ggataaacat
     4201 ggcgtatggc tgcgtcaggc cttcactact tcacaccgga agtcttccac cctgacacga
     4261 atataccgtc cgggctcttt cgcaggtgca aacagttttt cacattcggc gaatgtcatt
     4321 tctgaatcct ggtaacgcaa ccactgaacc ggcatattac cgggtttgat tattgtcgca
     4381 gttattttat atcgtttccc catatcgcct ctcatcctga caacaaaatt accggctgag
     4441 ccatcactcc ggtaaagcat gcgaaaaaaa gtgagcgtct cagcaatggc tcatgcgatg
     4501 attctgtacg ggcaccgatg gcacctcaga atacaacaaa gatatcagag taacatgtct
     4561 ctctcgttat ttttcagagt tcttgacacc ctccttctgc cacattgtca acgccagtgg
     4621 agacttctgc tgtcagtttt ttcatccata cagagtgaca ttaattatcc tgaacaaaac
     4681 gaaatatcct gaatatattt attttctcct ggtgccggag acatgatgat gtacaggctc
     4741 aggtgcggtg tgaatgtaaa atggtatgtt aattattcat ttctgaatgc aaactgaatg
     4801 caaacttatt ccgaattgaa ttctcctgac accacatgat aaaaatgagg atataataaa
     4861 atacttctct tctggatgtg ccattacctg ccgttgctgt aatacggcgg gtatttttta
     4921 ctgacatatc gggaagccag cagacgttcg tagctacatc cccgaaagtg gcacactgca
     4981 aactataata cgagtaaaac atcgatcacc gtagctgatg tgttcaacga tcgtgcacta
     5041 gcgttctgcg attctcaggg actgcctgtt ctgagaatcg gcgagtccgc gatcggatac
     5101 gatgcgattt cgcagtgact cagggattgc atctgaaaac cctccggcta tgtcggtaaa
     5161 tatttatttc tatgaaccgt acgattcaga tagcattcgc ccggccccgt aagaataatc
     5221 tccggacaaa taagattttt cctgaaaaag cgtctagtct tttgatatcg ttgtttttat
     5281 gctaatcagg gagtatgtgt gatgattgtc tgttacgtta ttcttgttaa ccgtccgttt
     5341 gaaacagaga ttgccgggaa aaagatattt tttcagaaaa atgacgtcct gttgacagat
     5401 aacatcagcc agtccttttt cctgcgccat cccggtaatg ttactgagct ccggattgat
     5461 gaatgtattg tcagaaaata tatggaaagt gccgacagga tccagcactc tgatagtgct
     5521 gtttctgttc cgttcattaa aacaaccttc tctcacatcg ctctgttaca taatgtcatg
     5581 cagaatctca gtctgcaaaa gacattgcca caacaatttt ctgagcagat gttttttgcc
     5641 tgcctttcca ttttttccac acacagaggt ttcatgtttc tgctattcag atgcataaac
     5701 tctgttgcgc ttaaagtcag gtatattatc tgttccagtc catcacgggc atggagagtt
     5761 aaagatatca gtaattctct gtacatgagc gagagtttac tgaaaagaaa acttcaggag
     5821 gaaaaagttt ctttctccca aatcctgctg gatatacgca tgcataacgc ccgacaactt
     5881 atttccagta aactgtcagt gaatcaaatc gcaatacggt gcggttatgc cagcacaccg
     5941 tattttatca gcgttttcag agaatatttt ggcataacac ctcatcagat gatgattaat
     6001 cagtccggat ttattgagag cagaatcgca attaagtaaa gtatgtcagt cagcgcataa
     6061 taaaagtatt cttatgcttc attatcagtt tctgtttatc actccggagc actgtagtaa
     6121 ctacgg
//
LOCUS       DAZGTJ010000168         6125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000168 DAZGTJ010000000
VERSION     DAZGTJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.168"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(125..604)
                     /locus_tag="ACHK8E_004896"
     CDS             complement(125..604)
                     /locus_tag="ACHK8E_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347026.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(687..1079)
                     /locus_tag="ACHK8E_004897"
     CDS             complement(687..1079)
                     /locus_tag="ACHK8E_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347027.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
     gene            complement(1545..1868)
                     /locus_tag="ACHK8E_004898"
     CDS             complement(1545..1868)
                     /locus_tag="ACHK8E_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347028.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(1901..2803)
                     /locus_tag="ACHK8E_004899"
     CDS             complement(1901..2803)
                     /locus_tag="ACHK8E_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0347029.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            4117..5073
                     /locus_tag="ACHK8E_004900"
     CDS             4117..5073
                     /locus_tag="ACHK8E_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347030.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNN
                     NPELFPHGFDIIIEDTTFQMYSPDREQQIKHVKKTLKYDGIFIFTEKFSTSTKEYQIR
                     EYQKDFSFKQRYFSQDEIDAKKENVLNIMNLNEVTLERMITAIKSHFKYYSVYWNSGN
                     FYSIAASNSQKNISDFIMLLPEAAIPMEYVYFKVPTRITGVI"
     gene            5078..5653
                     /locus_tag="ACHK8E_004901"
     CDS             5078..5653
                     /locus_tag="ACHK8E_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFP0347031.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKAN
                     IPFLIPMLNPKAFLFFAAFMPSFIKNNSNFNTDFLLLCIIFIIVSLLTDLIYICICLT
                     IRGRINSSFSKYVNFISSLFILLTGAYLVFL"
     gene            complement(5802..>6125)
                     /locus_tag="ACHK8E_004902"
     CDS             complement(5802..>6125)
                     /locus_tag="ACHK8E_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347032.1"
                     /translation="LKRYYKFYSNEEVDAIRNNGHANGKWAAAAISQNGHMWDAMPGS
                     YYKMGSDWDGGEFDDHLTLELEKNGKGSRIYITYASPAPGHLKDKFIEPLVKRVKDVA
                     EGRVR"
BASE COUNT         1821 a         1467 c         1140 g         1697 t
ORIGIN      
        1 caaaaacacg gtgcagtaag taaaagaaaa accgttacgg gagcaaaaca gccatcattg
       61 caaaatggca gttgcatcgc tcacgatttg acatccttcc tgtgactgac tggcatcctg
      121 cccgtcagtc tgcgtcggaa aattcaatac aggcctgctt caggagcccg gctatacgtt
      181 gattcagaca ttccgtccag ccatcgttcc atccacgctc ccatggattg cagacatcct
      241 cccatggatt gcagacatcg ttacagccat tcttccatgt cttccatgcc tccatctcca
      301 tgacagcccc cagcctgtgg cctttcacac gtatatgcca cagaaacggc cggaaaagtc
      361 ctgatttcag ttggtcacca ccgcttttgt agcccagatg acgtgcaata ctttcagtat
      421 attcaaatgc atcattctgc attccagccg ccttagccag cgagctgtac gcatcacgaa
      481 gtatcgtcat accttccagt tgatactcac accacgcacg gacagccgac actggtgcga
      541 ttacccgaag aatggtgcag gcacgccata caacaacgta aaggagtatt aacagactat
      601 gcatgtgttc acctcagata caaaaaataa cgggaacaca cctgccctgc gggaagcgtg
      661 ttcccgacag ggtaataaat gataaatcag cagggctgtg cgacgattcc atcaccctgc
      721 agaccaaacc ggtaacgcag ggtggtcagt tgctccgcca tacggcaggc agcatgccat
      781 gtcctgcgac gggatatccg tccggtaaac cggttcagat aaatgacctc acgcggtaac
      841 ggctgatacc atcctgtgta ctcgttgcga tacattccct gccggtaata caggcagggt
      901 gtccagacac cgccacgacg gaacatacag acccgcacgc tcagattgcc ggccggatta
      961 gtgtaccagc gactgtaata tgacaggtta ttgcgggaaa aaaacacccg caactgagct
     1021 gtcattcgcc ggaaaaaaga agagatgccc ggttcagggt acaaaataaa cgttgacata
     1081 aagactcctg taaaaaaacg ggagccttcg ccccgacgga caaaaactcc cgtcagggtt
     1141 aaaacaccac cagtacagca tggtgttgct gtcagaaaaa gcccgttaat aaagggcgca
     1201 gttataaaga aaccttcatc gtcatgactg acgatatctg ctctcaaagg tgggcagatt
     1261 taccaccacc agaaacatcc tggctgcaca tcccctttca ggttacgggg gtgttgatca
     1321 cccgaaggcg ttcctctcag atcaacggaa cattggtgcc caccgaagcg ggatgtggtc
     1381 acccgaaggc gctcctctca gaccacttat ggagcattgg tggtcgccga agcgacatgt
     1441 agccacccga aggcgctcct ctcaggccac ttgtggagca ttggtggtcg ccgaagcgac
     1501 atataagcct gtatattttt aaatcacaaa agatgaatgt caagttattc aacatcaatc
     1561 caaagaggaa tattatgtat tcccttaacg ggttctccca gaggaaaaac acaggtccct
     1621 ttaatcagta tttttcttcc cgaccatctg acatcacagt taaaatctcc caccacatca
     1681 aaataatccg caatggattc atagatatcc cattcatcat agatatccca tttatctgct
     1741 gttccttcac attcaaactc ggcctgatat ccggaatcaa catatcgcgc cagatagtgt
     1801 gaccacaaag gcgaattttt tgtaatctct tttacagaga ttattttcag caaaatagta
     1861 caatccataa tatttacccg tacgaaatcg ccatgagtta ttactcatag cctaacaact
     1921 gacaataaac ctgttcaata tatcgtcctc tgccatcacc atgaccaaga tcaattgatg
     1981 tcagtgcaag tgcctcctta taactgtaac cgttcataat atgatatttc atggcacttg
     2041 cagcgtaact gtaccgaaaa ctgtgtgcag tctcatctcc gcctttcagt tctccataac
     2101 gacgtaatgt attaagataa taatccattg ccgacttaag attatctctg tctataagac
     2161 gtccattatg ttcactggca tatttgagtg cataattaac agcatcaaga acgttatccc
     2221 ggtcaacaac cgttgtcatt ctttcccgtc cccctttcgt accaaaaatc actcttattt
     2281 tttcatctcc ccgttgtaac gcttttttcc aggttcgtaa tgatttggcc gactggatag
     2341 cttcttcatt tctcagaccg aggtaacgtg acagtttaac aacagcagcc acccctttat
     2401 cttttgcttc cacacgctga agaatctccc gaaaacgctc atccgtgatt aatttttttg
     2461 ttcccttacg actggagccg gaaatgccta acgctttatt gctgagacga ggatgttcgg
     2521 gatcagccat cttatattta cctgcctgac gtaacataac ccgtattgca gacatttcat
     2581 tttcccttgt tcgcccagaa acattactcc ggcttctcat atatagctca atatgccgga
     2641 cttttacctg cgcggcagaa ctgatctgta cattcatttt tgccagactt tcagcaaagc
     2701 ggtgcatgat cctggtgtaa tcactcagct tcttgaagct gcctttgtct tttcgaatgt
     2761 gagcaaccag atcctgtttc agtaattttt cacgtctgga catcaacttc tcctgcacat
     2821 aataattttt accttaaaca gctgtacgtt cggcagatga gtttttctgt tgtgccgtga
     2881 acacaacaga aaaactcatc tgcatttctc cctgacgaca cggttaaacg ttgcgatcct
     2941 tcgggtagtg actgcctgtg cgccagggta gcgcagattg tttaggtatt gaggggtaac
     3001 aaatcggtgt tgcacaccga ctgcagcatt tgctgtacct gtctccaggt cgcccccgac
     3061 accacctcta ataagtggta tcgccgattc gacatctcac tacataaaac tccttaaaaa
     3121 acaattgatt ctgcaaaaaa ccatttatgg acgttattca caaaatcgtc cgtaaatttg
     3181 cagaagaggt tgaaaaaaca ctctcacgcc gggtcacttc ttcaaatgtt aaaaaacatc
     3241 acgtgaaacg gcagttcgtg cgggttcgtt aagcagacgc gcgtgcgtca ctttgtcatc
     3301 gtgacacgct gacgaaagtg acgccgcgca gcttcagacg ccagatacac gcgtaagcga
     3361 ccaaatttca aatggtctta tacagcgcgc acagtcgcag gaagtgctgg caaacaaatg
     3421 aaaagaataa aagaagtcaa taccgccatt acgcagtaag aacattccgg catcaccgga
     3481 tggataagga tcacacaacc actgctgtgt attgcccgta ataaagggcg cggttaaaag
     3541 aaaccttcat cgtcatgact gacgatatct gctctcaaag gtgggcagat ttaccaccac
     3601 cagaaacatc ctggctgcac atcccctttc aggttacggg ggtgttgatc acccgaaggc
     3661 gttcctctca gatcaacgga acattggtgc ccaccgaagc gggatgtggt cacccgaagg
     3721 cgctcctctc agaccactta tggagcattg gtggtcgccg aagcgacaga taatgcattc
     3781 tgagcatatc agagcgcata aaaggcgtca attatccatg ctccctcacg aaacagaagc
     3841 tgaactctta acacctgcta tcggaactca ctgaataaac aactaaaaag gacattgtga
     3901 aaattaacat caacagtatg taaagtgatt tgttttgtct taaatgggtg aatgtaaaaa
     3961 aatattctat acaatccaca catccttgta tcatatatca agtaaacatc cacagagata
     4021 acagcttatt tcaaataagc agattctgtt tgatacttca gaaaaatagt atatgctcct
     4081 gatttactga tgttataagt tatacaggat agttaaatgt gtgattttat aaaatcatgt
     4141 tatgcagcac caagaattaa caaaatggct gattttttct catcaacagc atctggagta
     4201 aaacataaac ctttgaagca tgtaaaaatt gataacaggc ttttcccatc tcctgagtat
     4261 ataatttttt ctgactatct tacgaaaaac agtggtccat ttgttaacca ctacttttgc
     4321 agtattccat acagcaaaga agaagaatgc aggctgggtg tgactattat gaaatatgca
     4381 agccatcgaa ataagccatt gagcttatgg tgtcttggaa tggcagaagg agccatggcc
     4441 agaacaattt ctcaactatc aaaaggacag gttttatcat taacaaccag cccaacaaaa
     4501 gaaaatgaac ctgtattctt tagtcatggt tctcatattc actcttactt tatttgctcc
     4561 ccattctttc ttgttcatga agaaataaaa aataacaacc ctgaactatt tcctcatggt
     4621 tttgatatta taatagaaga tacaacgttt cagatgtatt ctcccgacag agaacaacaa
     4681 ataaaacatg taaaaaaaac attaaaatac gatggtattt tcattttcac agagaaattt
     4741 tccacctcca caaaagaata tcagattaga gaatatcaaa aagatttttc atttaaacaa
     4801 agatattttt cacaagatga aatagacgca aaaaaagaaa acgttcttaa tatcatgaat
     4861 ttaaatgaag taactctcga aagaatgatt acagccatta agtcacattt taaatattat
     4921 tctgtctact ggaatagtgg taatttctat tcaatagctg ccagcaattc gcaaaaaaac
     4981 atttctgact ttataatgtt gcttcctgag gcagccatac ctatggagta tgtttatttt
     5041 aaggttccaa ccagaataac aggagttata taatcccatg gacttctcaa cattaataac
     5101 attctctatt ataaccttat cacaaacagt atcgattggt ccgggggtgg cattaatcat
     5161 atctgaaagt ctgacatatg gtgcaaaaaa agcaataacg acaagtatat atataagatt
     5221 tggcgaaatt gttgtcatgc tagtcacact attttctata gcactggcaa tcaatttaga
     5281 aaaacatctt gacacaataa aaacttgtgg tggtggatat ctgatttttc ttggtattac
     5341 tcgtctcatc aagagaaaag gatctaaagg aaacaaagca aatatccctt ttttaatacc
     5401 gatgctcaat ccaaaggcct ttttgttctt tgctgcattt atgccatcat tcataaaaaa
     5461 caacagtaac tttaatactg actttttgtt actttgtatc atttttataa ttgtgtcatt
     5521 actcactgac ttaatatata tatgtatatg tttaactata agagggcgta taaattcatc
     5581 attttcaaaa tatgttaatt ttatcagctc tttatttata cttttaactg gcgcatatct
     5641 ggttttttta tgatatgccg tcagggtgtg cttccttaca tatcacgccc tcttccagac
     5701 gtcgctggtc gtcgccctct gcgtccctta ctttcagccc ctctgagcat aatcgcccct
     5761 ccagaggagc ccatcacaac gacaatgcca gcgggttaat tttatctgac ccggccttca
     5821 gccacatcct tcacacgttt aactaatggc tcaataaatt tatctttcag atgccccggt
     5881 gccggagatg catacgttat atatatccga cttcctttgc catttttttc aagttcaagc
     5941 gtcagatgat catcaaattc gccaccatcc cagtcactgc ccattttata ataactaccg
     6001 ggcatggcat cccacatatg cccgttctga cttattgctg ctgcggccca cttcccgttc
     6061 gcatgtccgt tattacgaat cgcatcgact tcttcatttg aataaaactt atagtaccgt
     6121 ttcag
//
LOCUS       DAZGTJ010000169         6106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000169 DAZGTJ010000000
VERSION     DAZGTJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.169"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..1540
                     /locus_tag="ACHK8E_004903"
     CDS             173..1540
                     /locus_tag="ACHK8E_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0347033.1"
                     /translation="MEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVTLCITLFFVLG
                     GPIVAHIFSSHYESYDLHIAELTNKNGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKKPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            1817..3025
                     /locus_tag="ACHK8E_004904"
     CDS             1817..3025
                     /locus_tag="ACHK8E_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0347034.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
     gene            complement(3249..4814)
                     /locus_tag="ACHK8E_004905"
     CDS             complement(3249..4814)
                     /locus_tag="ACHK8E_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFP0347035.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKYRN
                     EEQTKIWNITTKN"
     gene            complement(4830..5078)
                     /locus_tag="ACHK8E_004906"
     CDS             complement(4830..5078)
                     /locus_tag="ACHK8E_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0347036.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            5179..>6106
                     /locus_tag="ACHK8E_004907"
     CDS             5179..>6106
                     /locus_tag="ACHK8E_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347037.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVYSTLKSEYIRNYFLKSFGVVF
                     IVLDIYNTYRPENETGFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGF
                     NREDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELD
                     RILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLY
                     DFNIFDTTPYARALLERFHEFESGPKGALTRYI"
BASE COUNT         1689 a         1127 c         1417 g         1873 t
ORIGIN      
        1 caagcctgcc ttgcagcaaa aaatgtataa agcattaaag tgataataat tacattctgc
       61 aactatcatc atcggtattg tttgtgggtg gattttcata ttgacaacag tacagttctt
      121 atatctatta ataataaaaa gggatctaca acctacaggt tggtgtagct ttatggaaaa
      181 agactatttg ggaattagta gtactgtatt agtgagctta ttgtttgggc ttgcattggt
      241 tttggttaat agttggttca atcagccaac cgttgaggaa gttgttccac gatcaaccta
      301 tctgatggtt atgattgctt tattttttat cgatactgtt gcatttattt ttatgcagtt
      361 gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg cttttctaag
      421 ttgtttgatt tactttatta aaaccgtcat tatcattcag caaattattg aggggcgttt
      481 gacaagcagt gttgttcaaa atgacattgc aatttattat ttgtttcgtc agatgagttt
      541 gtgcgtatta atatttctgg cattggtaaa taaagttagt gaaaacacaa aacagcgcaa
      601 tttgttttcc aaaaaagtga ctttgtgcat aactttgttt tttgttcttg gggggccgat
      661 agttgctcac atattttcta gccattatga gagctacgat ttacacattg ccgagttaac
      721 caataaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga ttttcatgtg
      781 gttaaccttg ctttcggtta atctatactt taatggttta cgctgtgata tctggaacgg
      841 ggtgtcggtg attgcttttt gtgccgtgct gtacaatatc tctctgttat ttatgagcag
      901 gtatagcgtg tccatttggt atattagtcg cactattgaa gtagtcagta agttaaccgt
      961 tttggtaatt tttatgtgcc atatattcag tgcgctacga gtaacaaagg acattgcaca
     1021 tcgcgatccc ttaaccaata tatttaacag aaattatttt tttaatgaac tgacggttca
     1081 atcagcatcg gccaaaaaaa aaccttattg cgtcatgatt atggatattg accacttcaa
     1141 aaaagtcaac gacacctggg gacatccggt tggcgatcaa gtgataaaga cagtggtgaa
     1201 tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgttggcg gcgaagagtt
     1261 tggcgtcttg ctgacggaga tcgatactga atgtgcgaaa gctttagcgg aaaggattcg
     1321 ggaaaatgtt gaacgtttaa ctggcgataa tcctgaatac gccataccac aaaaagtgac
     1381 aattagtatt ggcgctgttg taacgcagaa aaatgagtta aaccctaagg agatctatca
     1441 actggctgat aacgcacttt acgaggcgaa agagaccggg cgtaataagg tggtagtaaa
     1501 agaggcagaa attcttatca accgcaagga tgatgaatga gtctgtcgct agttgtcgac
     1561 ctccgcctgg ggcttctcat cccccagtgt gtgaaatata cgaaaaaaag ctcgcacttt
     1621 cgtacgagct cttctttaaa tatggcggtg agggggaccg cctgaaataa atctgactta
     1681 atccgggagt gttatataac ttcattataa acaatgcatt acaatttttg ctgtccgatg
     1741 ttatctaatg tattcttagt gaagtgagag actaatggca tactggatgg cataccagag
     1801 taacaggaat tagcgtatgg cagtattgac ggatactaaa gcaaggcata tcaaacctga
     1861 tgacaaacca ttgccccatg ggggaattac aggactgact cttcatcctt cttcagtaaa
     1921 ggggcggggg aaatgggttt ttcgttatgt aagtccggtg acacaaaaaa ggcgtaatgc
     1981 tggattgggt acttaccctg aggtcagtat tgctgaagct gcacgtactg cccggataat
     2041 gcgagagcaa cttgctgcag gtgatgatcc tctggagatt aaaaaggctg aagctgaaaa
     2101 agttgttatc ccaacatttg ccgatgcagc ccggcgtgta catgcagaat tgtcaccggg
     2161 atgggaaaac ccaaagcatg tcaggcagtg gttgtcgacg cttgaaaatt acgtgtttcc
     2221 tcaactggga gcaaaaacgc tggatacgat tacggctgcg gacgtggcag agacactgcg
     2281 tccagtctgg ttgaccttat cagaaacggc aagccgggtt aaacagcgca ttcatgttgt
     2341 tatgcagtgg ggctgggcgc atggtttttg tgtggcgaat cctgttgatg tggttgatca
     2401 tttgcttcca cagcaatcaa gaggacgtga tgaacaccag ccggcaatgc cctggaggca
     2461 gttaccgctt tttgtggcga ccagtgtgta tacagatgaa ccttataatg ttacccgggc
     2521 actgttatta atggtgatac tgacagcaac ccgctcgggc gaagcaaggg gaatgcgctg
     2581 ggctgaaatt gattttcata agcggatatg gacgataccc gcagaaagaa tgaaagccag
     2641 gatacagcat cgtgttcctt tatcccgaca ggccattcac gttctggaaa atatacgtgg
     2701 tctgcatgac gaactggtgt ttccttctcc cagaaagcag cagatccttt cagatatggt
     2761 gttgacgagt tttctacgta aaaagaaggc catcagtgat atacccggac gagtggctac
     2821 agcacatggt tttcgttcaa cattcaggga ctggtgtagc gaacagggat attcgcggga
     2881 tttggcggaa agggcgcttg cccacacgct gaaaaataag gttgaggcgg catatcaccg
     2941 gactgatctg ctggatcagc gtataccgat gatgcaggca tgggcggatt atgtgatgtc
     3001 tcagattatg gaaaaccagc gatgaaatca catctcacag atttgcagat gtcgtctgta
     3061 atcacgaaaa atatacttat tgagttataa tgcttgtttg tctgatattt gcaatgacat
     3121 aaatgtaaaa ataatacgta atattatagt ttataaattg aatgataaaa acaatgccag
     3181 tctgtaagtg atgtctggca ttgtttcaga tattcaggtt ggtaactgaa tgataaaaga
     3241 cgggaacttt aattttttgt agttatgttc catattttag tctgttcttc atttctgtac
     3301 ttattcagct tatggtccca acagaccata aacattccac gctccattat cgggatgttt
     3361 gcctcatttg ctaattcaca aggtgtaatc gcgtccccga tataaatgat tttttggccg
     3421 attgaataat caattacgat ttttttctgt tctgcaagta tatcaagtgc ttcatcaaga
     3481 acacatttat tcctgagcgc atatggacca tattgtagta tatggctttt ttgatattcg
     3541 tatcctttta cccggatgat attttctctg agccatttat ccagtttttc tgcaggagat
     3601 atttcccgaa gtgattccag tttggatata agatgctgaa aataaaaatc aacgagataa
     3661 atagcactat tgatactttc tcgtgttatt actgtaccat gtggattgtt ctccagctcc
     3721 agaagtgcgg ctattcgaac agcctgttct cttttcctga cgataatgtc ttcgaaagtg
     3781 gtcatacgtg ggttctgcct gactgcactc atattttctt tgtgaaagat ttccagatcg
     3841 tgggatgctg cgtttgataa tgtcatgcag atgcgttgct caccttttgc acgcctttcc
     3901 cgggacgctc tcagagagtc tctgatctga ctgccaaaac ttttctcatg aaaacaggtt
     3961 gcacattgct gcgttacaac aggagtggca gtatgcggtt ctgtcgtcat tattttattt
     4021 gctccgggtt cgcaaaatag cacccgggcc agaaagccat tgccccggat ctggtcgcca
     4081 gtgagagtaa aaaacttatc aaatattacc ggttgagtga gtataagcat agtgagacgg
     4141 gaatccttta cccggaaccc gggtctggat gcccgttcta cggtgactgg ttgcccactc
     4201 cataatgtgt tgtacagcgc cgatttgcgt tcgaaacgct tatccagaat agttcctcct
     4261 tcatcagaca gcaacagaag agaaggtgac tggctgagtt tcaaagctat cccttctggt
     4321 gtcgcgtcat tcacgatgag aacctgttgt acgggaggta cgggtttatt tgctatacac
     4381 ttttcaagcg ctgctcgtgc agcagcagca ttttccgggg atctttccat ttctttttta
     4441 tatctccgtt ccaggatttt tttatctgtt gtccacattt ccatcatgcg atcatagttt
     4501 tttttatcgt cgatatatgc ctgatgtagt tcttcttctg tttcatgaac aggggcgatt
     4561 aattttgtgc aggcagaggt ttttccactg cctgatgctg atgaaagaca catatatatc
     4621 tgaagactgg tacgctgccc tccagaaagt tcaacttcat gcgtatcctg gaccgaggca
     4681 gccttgaccg tcagcataat agacagcagt atttcacggc ttattcccgt ttcttcatgc
     4741 atttcatcaa gtaatgcctg gcacctgtca ggcagtttag ttatgtcaag ttcagggggg
     4801 gataattttg tcatggcgta cagttttcct tatttatcct tagttacgga tctggttggt
     4861 gttgtatgta aagttaacca ctcatcaatg gcagatgccc gccagcctac agccgaactc
     4921 ccaagccgga caggccgggg aaaggtggca tcgtagtatt ttgatgccgg attcattttt
     4981 tcgtagatgg tcgatcttga tataccaagg agccgggtaa gttcagccat acgcaggatg
     5041 cgtgaggggg tataaaaatg tccggtctgg gttgacatat tgttctccat gtgagttgaa
     5101 ggctatgtca ttaatttgct aggttaaggc cagaatgtcc cctgtgataa aacggaaacc
     5161 aaccggagat cagagcagat gcgcatgttt acaaatcttt tgtatgatat ttgtacagtt
     5221 tttgagttgt ttaaggaagg agagagtccg agagataagc ggaagagtac tgattttgga
     5281 gcgcatcaaa gattctggga tcaacgttat aatgagctca gtcacattat tgatgctgaa
     5341 ggggtgtata gtctggaaca acggcgaatt atattttccc ggtatgaata tttttattac
     5401 atgatgaatt cttaccctgt gtattcaaca ttaaagtccg aatatatccg taactatttt
     5461 ctgaaaagct ttggagttgt ttttattgtg ctggatatat ataacacata tcgtccggag
     5521 aatgaaacag ggttttatta tcatatctat aattttttgc aaaaatcata ttgtccctgc
     5581 ctggattact ctggtacaga aagcgatgaa gctgccgtta aacgttatct ccgggagtat
     5641 cttgctgaac tgggatttaa cagagaagat tttcgggaaa acggaaaaat gtatgagctg
     5701 ggaaaatacc agggaactat tcgaaaaggg tacggtaaaa ggaagagcct tatgaaacaa
     5761 tatataaagg cctgtaagaa tgaatacaag aaggattaca gagagaagaa gcttgataag
     5821 agtgagctgg acagaatact caacaacatt gacaagttct attatgcatt ttattcatta
     5881 agtattttgc ttgatatgca acggaaggta aagatacttg acagtattgc atattatctc
     5941 agagtgctga taagagaagg gctttgggta catgggttgt atggttacgc tgcacgctat
     6001 ctttatgatt ttaatatttt tgacacaacg ccttatgcaa gagcattact tgaacggttt
     6061 catgagtttg agagtggccc caaaggagca ttaactcgtt atattg
//
LOCUS       DAZGTJ010000170         6095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000170 DAZGTJ010000000
VERSION     DAZGTJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.170"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            142..1476
                     /gene="adeQ"
                     /locus_tag="ACHK8E_004908"
                     /gene_synonym="yicO"
     CDS             142..1476
                     /gene="adeQ"
                     /locus_tag="ACHK8E_004908"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP0347038.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1529..1981
                     /locus_tag="ACHK8E_004909"
     CDS             1529..1981
                     /locus_tag="ACHK8E_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP0347039.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2192..3382
                     /gene="nepI"
                     /locus_tag="ACHK8E_004910"
     CDS             2192..3382
                     /gene="nepI"
                     /locus_tag="ACHK8E_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP0347040.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3423..3716)
                     /gene="yicS"
                     /locus_tag="ACHK8E_004911"
     CDS             complement(3423..3716)
                     /gene="yicS"
                     /locus_tag="ACHK8E_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP0347041.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3938..4756
                     /gene="nlpA"
                     /locus_tag="ACHK8E_004912"
     CDS             3938..4756
                     /gene="nlpA"
                     /locus_tag="ACHK8E_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP0347042.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4760..5683)
                     /locus_tag="ACHK8E_004913"
     CDS             complement(4760..5683)
                     /locus_tag="ACHK8E_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP0347043.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5850..>6095)
                     /locus_tag="ACHK8E_004914"
     CDS             complement(5850..>6095)
                     /locus_tag="ACHK8E_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347044.1"
                     /translation="ETIDKINKENIKEIKLKNEKTYVSLPNPTILITTTSTPERQFSI
                     SVLDDDNKENLPTDTAPCFTIGIPDEYTKAIELLNIK"
BASE COUNT         1477 a         1340 c         1545 g         1733 t
ORIGIN      
        1 ttatttatca taaagaaacg tcgcgagatt attaaagaat atccattaat gtgcaattga
       61 aatgtgataa ttatcactga accgttgttg tcatttgttt tacaaaagca agggattaaa
      121 tctcctccaa tggacaaaaa aatgaataat gacaataccg attacgtgag taatgaatca
      181 gggacgcttt cgcgattatt taaactacct cagcatggga ccaccgtccg cacagaattg
      241 attgcgggga tgaccacttt tttaaccatg gtatacatcg tttttgtgaa tccgcaaatc
      301 ctcggcgcgg cacaaatgga cccgaaagtg gtgtttgtta ccacctgttt gattgccggt
      361 atcggcagta ttgcgatggg gatatttgct aacttacccg tggcgctggc tccggcaatg
      421 gggctgaacg ccttctttgc gttcgtggtc gtgggggcga tgggcatctc ctggcagacc
      481 gggatgggcg cgatattctg gggcgcagtt gggctatttc tgctcacgct gtttcgtatc
      541 cggtactgga tgatctccaa cattccatta agtttacgta ttggtatcac cagcggaatc
      601 ggattattta tcgccttaat gggattaaaa aatactggcg ttattgtcgc caataaagac
      661 accctggtga tgattggcga tttaagttct cacggcgtgt tgttaggtat tttagggttt
      721 tttattataa ccgtgttgtc atcacgtcat tttcatgccg cggtgcttgt ttctattgtg
      781 gtgacgtctt gctgtggatt atttttcggt gatgttcatt ttagcggcgt ctattccatt
      841 ccgcctgata ttagcagcgt cattggtgaa gtggatttga gcggcgcgtt atcacttgaa
      901 ctcgccggta tcattttctc ctttatgctg atcaacctat ttgattcatc aggaacatta
      961 attggtgtaa ctgataaagc tggcttaata gatagtaacg gtaaattccc taatatgaat
     1021 aaggcgctgt atgttgatag cgtcagttcg gtggcgggtg cgtttatcgg cacctcgtct
     1081 gttaccgcct atattgaaag tacttctggt gtggcagtcg gtggtcgcac ggggctgact
     1141 gcggttgtgg tcggcgttat gttcctgttg gttatgttct tctcaccgct ggtggcgatg
     1201 gttcctcctt acgcaaccgc cggagcgtta atctttgttg gcgtgttgat gacctcaagt
     1261 ctggcgcgcg ttaactggga tgattttacc gaatcggtac ctgcgtttat taccacggtg
     1321 atgatgccct ttactttctc gatcaccgaa gggattgcac tcggctttat gtcgtactgc
     1381 atcatgaaag tgtgcaccgg gcgctggcgc gatctgaacc tgtgtgtggt ggtggtcgca
     1441 gctctgtttg cactgaagat tattctggtg gattagtaac tggcgagggt ttccagatat
     1501 catgagttct gattacgcag gagaactcat gatctggata atgctcgcca cgctggcggt
     1561 agtgtttgtg gttggttttc gggtgctgac atccggggcc agaaaagcga ttcgccgtct
     1621 cagcgatcgg ctgaacatcg atgtcgtacc cgtggagtcg atggtcgatc aaatgggaaa
     1681 gtcagccggt gacgaatttt tacgttattt gcatcgtccg gatgagtcgc acctgcaaaa
     1741 cgccgcgcaa gtgttgctca tctggcaaat tgtcattgtc gatggtagcg aacagaactt
     1801 gctgcaatgg catcggattt tacaaaaagc ccgccttgcc gctcccatta ccgatgctca
     1861 ggtcaggctg gcgctgggtt ttctgcgcga aaccgaacct gaaatgcagg atattaatgc
     1921 ttttcagatg cgctataacg cgttctttca gcctgccgag ggcgttcact ggctgcatta
     1981 aaaccgcttc gtcttatcag cctacagacc gcgcctgacc tgtagggtgg ataaggcgtt
     2041 cacgccgcat ccggtaatct ttgccctgtt ccgtttcgat cttaactcca gctcaagcaa
     2101 caccgtaaaa gttgcaaaaa cgttaaatac gtcacacatt tcaatgttaa agtggtcggc
     2161 tttccctctg aaacatgcca cgggtaacac catgagtgaa tttattgctg aaaaccgcgg
     2221 cgcggatgcc atcacccgac cgaactggtc agccgttttc tcggtggcgt tttgcgtcgc
     2281 ctgtctgatt atcgttgagt ttttgcctgt cagtttgttg acgccaatgg cccaggattt
     2341 aggcatttcg gaaggggttg ccgggcaatc ggtgaccgtg accgcctttg tggcgatgtt
     2401 tgccagtttg tttattaccc agaccattca ggccactgac cgccgctacg ttgtcattct
     2461 gttcgccgtt ttgctgacgc tttcctgttt gctggtttcc tttgccaact cattcagtct
     2521 gctgttaatc ggtcgcgcct gtctggggct ggcgctgggc gggttctggg cgatgtcggc
     2581 gtcgctgacc atgcgtctgg taccgccgcg tacggtgccg aaggcgctgt cggtgatctt
     2641 cggcgcggtt tctattgcgc tggtgattgc cgcgccgttg ggcagttttt taggcgagct
     2701 tatcggttgg cgcaatgtct ttaatgcggc ggcggcgatg ggtgtgctgt gtattttctg
     2761 gattatcaaa tcactgcctt cgctgccagg tgaaccttcg catcagaaac aaaatacttt
     2821 ccgcttatta caacgtccgg gagtgatggc cgggatgatc gccatcttta tgtcctttgc
     2881 cggacagttt gcttttttca cttatattcg cccagtgtat atgaatctgg cgggattcgg
     2941 tgttgatggc ttaaccctgg tgctgctgag ttttggtatc gccagctttg tcgggacatc
     3001 gctttcgtcg tttattctca aacgttcggt gaaactggcc ttagcgggcg caccgtttgt
     3061 gctcgccctg agcgcgctgg tactgacgct gtggggaagc gataaaattg ttgctactgg
     3121 tgtggcgatt atctgggggc tgacctttgc tttaattccc gttggttggt caacgtggat
     3181 cactcgctca ttggccgatc aggcagaaaa ggctggatct attcaggtgg ccgttattca
     3241 gcttgccaat acctgcggtg cggcaatcgg cggttatgcg ttggataaca ttggtctgac
     3301 ttcgccgctg atgttgtccg gcacattgat gttgctgact gcattgttgg tgactgcaaa
     3361 ggtgaaaatg aagaaatcct gatatcaggt aaagaagcgt tggtcagtgg ttgttgccgt
     3421 tcttacatat ccgggcattc taccttaccc aacgcttccc aataattgct ctgattatta
     3481 cgttccatcg ccacgatggc ctcgcgtata tgttgtttat tgttttcatc tgacaacatt
     3541 aacttttccc acgcttcatc tgataatgac gcctgtggcg tgcaaatttt acgtaaatct
     3601 tgcaataccg tggttttctc ttttgccgac tgtagagaac tgaatggcga ctcggcctga
     3661 gaaatggctg gaaaaatcag gaatacggta atcatgagta gcatcgttgg cttcatagcg
     3721 gcaaccttct gcaaatgtgc atcgaatgaa gcataagtgt agtttgcttt tgccgctctc
     3781 atcatgctgt acgaaaggtg tttgcgtggt cagtaagaag tgccagactt tatattccac
     3841 ttttattcct ttttattctt atcgatagcg tttcgttttt taaactgcag cgaccttacc
     3901 gctatagtca ggtaatcatt aataaaagga taaaaaaatg aaactgacaa cacatcatct
     3961 acgggcgggg gccgcattat tactggcagg aattctgctg gcaggttgcg accagagcag
     4021 cagcgatgaa aagcacatta aagtgggcgt tattaatggc gcagaacaag atgtcgcgga
     4081 agtcgctaaa aaggtggcaa aagagaaata tggcctcgat gttgaactgg taggatttag
     4141 cggttcgttg ctaccgaacg atgcgacaaa tcatggcgag cttgatgcga acgtcttcca
     4201 gcatcgaccc tttcttgaac aagataatca ggcgcatggt tataaactgg tagcggtggg
     4261 aaatacgttt gtcttcccca tggcgggtta ttccaaaaaa atcaaaaccg tggcgcaaat
     4321 aaaagaaggc gcgacagtgg cgatccccaa cgaccctacc aaccttggtc gcgcgctttt
     4381 actgttgcaa aaagagaagc taattactct gaaagaggga aaagggttac tacctaccgc
     4441 gctggatata accgataatc cacgccattt gcagataatg gagctggaag gggcacagtt
     4501 gccacgtgta ctggacgacc cgaaagtgga tgtagcgatt atcagcacca cttacatcca
     4561 gcaaacagga ctttctccgg ttaacgacag cgtatttatt gaagataaaa attcgccgta
     4621 tgtgaatatt ctggtggcac gggaagataa taagaatgca gaaaacgtga aggaatttct
     4681 gcaatcttat caatcacccg aagtcgctaa agcggcagaa accatcttta acggaggcgc
     4741 ggtgcctggc tggtaattct cactcatgcc gcgccggacg atttattttt ctggcgcggc
     4801 ggcgggaatc cattgaaatc aaaatcaccg atgacagaat cagcagcgtt cccagccagt
     4861 ccggcaatgt gaaggtgatc cccagcaaca gcaaagagag cagcgcgcta cttaatggtt
     4921 ctgcacagct taaaatgctg gcttttggac cgccaattaa ttgtgctcct ttcaggtaca
     4981 gactaaatgt cagggacgta ccaatgacca ccaaataaaa aaacgccaga atcaaactgc
     5041 cgttaacgac aaagtttgtc ccttgtctgg cataaaaggg cagcaggatc agaccgccaa
     5101 tcagcatact ccagccgacg accggtaacg tgccatagcg ggcaatcagc gttgagggat
     5161 aggtggtata gaatgcagca gcaaatgccg aggcaatgcc ccagaataac gcggcaggag
     5221 agatcgataa tgacgtcgga ttaccgtgtg tcaccaataa aaaagtcccg atcagcgatg
     5281 tcaaaatagc gcagaaaacc agaatgcccg ggcgggattt acgcaccagt gagaaccagg
     5341 cgacgataat agtcggtgag aggaattgca gcaccgttgc cgtggctgcg ttcgattttt
     5401 ctatggttag caaaaaagtg agctgtacgg ttagcgcgcc aaccacggaa aaaatcagta
     5461 ggctaatggc atctttgtga ttgttaataa tagaaaagat tttatcacca tgaacaaatg
     5521 atagcgtcag cagaatcaaa ccggcgaata tcaaacgcgt catagtcaaa aactgcgacg
     5581 acatctggct ttgctccatg atgtattgcg cgcagacccc tgaacttccc cacaacacgg
     5641 cggcaatcag aacgttcagc atcccctttc tggtggaacc catttttccc tctgcatact
     5701 gttttgtctt ttcatcaata tacgttaagg ataccactac cgatgacggt gagggagtag
     5761 agtaatcaat cagttttagt gaatggagga aataatgaca tataacaatt attcatatgg
     5821 tatttaaaaa aactcaatat gaaatttaat tatttaatgt tcaataactc tatggcttta
     5881 gtatattcat caggaatacc aattgtaaaa caaggtgcgg tatcggtagg tagattttct
     5941 ttgttatcat catccagaac agaaatagaa aactgtcgtt caggtgttga tgtcgtggtt
     6001 attaaaatgg ttggattagg aagagaaaca tatgttttct cattttttag ttttatttct
     6061 ttgatgtttt ctttgttaat tttatcaatc gtttc
//
LOCUS       DAZGTJ010000171         6053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000171 DAZGTJ010000000
VERSION     DAZGTJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.171"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..906
                     /locus_tag="ACHK8E_004915"
     CDS             <1..906
                     /locus_tag="ACHK8E_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0347045.1"
                     /translation="SIAQRLRAANTGLTSGNGPQGVFLLVGPSGVGKTETALALADVM
                     YGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVE
                     KAHRDVLNLFYQMFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTES
                     ELHELIRPLLRDHFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLST
                     SVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWN
                     DEDGIVIAFDGINSDYPQESIYGQC"
     gene            893..1534
                     /locus_tag="ACHK8E_004916"
     CDS             893..1534
                     /locus_tag="ACHK8E_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347046.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            1531..1758
                     /locus_tag="ACHK8E_004917"
     CDS             1531..1758
                     /locus_tag="ACHK8E_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347047.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            1837..4356
                     /locus_tag="ACHK8E_004918"
     CDS             1837..4356
                     /locus_tag="ACHK8E_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0347048.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            4353..6035
                     /gene="tle1"
                     /locus_tag="ACHK8E_004919"
     CDS             4353..6035
                     /gene="tle1"
                     /locus_tag="ACHK8E_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFP0347049.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
BASE COUNT         1605 a         1236 c         1641 g         1571 t
ORIGIN      
        1 tccattgcgc agcgtctgcg tgcggcgaat acgggtctga catccggtaa cggcccccag
       61 ggcgtgttcc tgctggtcgg tcccagtggt gtgggaaaaa ccgaaacggc actggcgctg
      121 gcagatgtga tgtacggcgg cgaaaaatca ctgattacca tcaatctgtc ggaataccag
      181 gagccccata cggtttccca gcttaagggg tcaccgcccg ggtacgtggg atacggtcag
      241 ggcggtattc tgacggaggc tgtgcgtaaa cgcccttaca gcgtggtgct gctggatgaa
      301 gtggaaaagg cccaccggga tgtgctgaat ctgttctacc agatgtttga ccggggcttt
      361 atgcgcgacg gtgaggggcg tgaaattgac ttccgtaata cggtcattct gatgacctcc
      421 aatctgggca gtgaccttct gatgcagcaa ctgagcgaaa agccggagac aacggaatcg
      481 gagctgcatg agcttatccg tccactgctg cgcgaccact tccagccagc actgcttgcc
      541 cgtttccaga ccgtgattta ccgtccgctg acgcagtctg ccatgcgcat cattgtggag
      601 atgaagcttg cacaggtctg tgaacgcctg cactgccatt atggactgag cacatcggtt
      661 gatgaacgtg tgtacgatgc cctgacatct gcctgcctgc tgccggatac gggtgcccgg
      721 aatgtggaga gtctgctgaa ccagcaactg ctgccggtac tgagccggca gttgctcagc
      781 tatcttgctg ccagacagca tccgacgtac ctgtctctga cgtggaatga tgaggatggt
      841 attgtgatag cgtttgatgg aatcaatagc gattatccgc aggaaagcat atatggacag
      901 tgctaaagaa attgcccggg atatgctgga ggccgtaaat attaatgggg atgggtttct
      961 gggggccgtc gggaagggac tcatatcttt gccagtcagt ctcggttata tgggatatga
     1021 ttttatagat accaggcata ggcgggaaaa ccagaatgac aaattcagaa tggccagact
     1081 ggtagaaaaa ggcattttca gccgtgatat tattgggaaa attatcagta tttgtcttga
     1141 agactttgta tcccggatca atatggaaaa aacaggctcc attgtgaaga acgtttcagg
     1201 agatctggca ggtagaatgg catttgctga attgaccggg gttaaactgg gagaggttct
     1261 ggtatccaga gggctggctg ctttttttgc agggagtttt gccgggttgt tattatctgt
     1321 tggcgcagag acatcgcgtg cgatttacac atcccgtttt ctgaaagaca gaaatccggt
     1381 gttgcatgat aagctgaaag ctcaggggga tttggatctt ttgtactttc ttgttgaaga
     1441 tattgtgcgc ccgtttgaac gagcctgtat tgtcgcagac cgaaattcgg ctgagtttga
     1501 ccggatatgc gaatattttt tcggtggttt atgaagataa aaagaacatt acttataatg
     1561 ctttcccgat ttatccgtgg cgcaggtatg gggttaggtg cgtcgggtat tgtatttaca
     1621 gtctggttct tttttctctc atctgctgaa tttaaatatc tgtggggagg gctctctgtt
     1681 gcgttatttt ttgtcggtta tctgatgtat cgatttgcat atgcatatat ttacgatgaa
     1741 tgggatgatt atcagtaatt ctctctgaaa ataaagctta acggtcatca ttatataatt
     1801 gcagttcaat accggtacag acatggagtt aacaatatga gtctgacaga tacactttca
     1861 aaaactatct ctgcttccct gaatcgttac catttaatta ttccctcctg cccgtcacca
     1921 ctggatgttg agcatttcag cggcagggag gagatgagtc agacctatta ttacatcatc
     1981 aattttacca gtactgacaa cgacctggat gccgggctgc tgctgcgtaa atcagccaca
     2041 tttacaatgg gtacgggagg gcttaaggag caggtgagcc agaaagtagt acatggtgtc
     2101 attacagatt ttcgccgtat atcgggctcc gccgaccagg caaaatacca ggtgatactg
     2161 gaaccattta ttaaactact ggacaggcag catcgttctc accgattttt cgttaacaaa
     2221 tcggtgccgg aggtggtgac agaggttctc caggagcacg gcctgaaagg ctgggagttt
     2281 gagttccgtc tcaaaaaaac gtatccgaag cgtgaacaga ttaaccagta tcaggaaagt
     2341 gacctgcaat ttattcagcg tttgctggct gaagtgggga ttttttattt tttcaccctg
     2401 caaccggaca cgcagacgga agtagtgcat tttggtgaca gccaggctgc gctgacgttt
     2461 gataagacgc tggcgataaa cagtccgtcc ggaatgaatg ataacagagc ggattcagtg
     2521 tgggggctga atgtgacgca caatgtggca gaggccagtg tcaccacaaa ggcttataac
     2581 catcgtgaag cacaatatct gttgcagtcc gccccggcag acatgacgca tggtgatggt
     2641 gacggaatta actatggtga ggtgtaccac taccatttcc gtcatctgga acggggggat
     2701 aaaatcgacc cggtgccgga gacggccaat ttttatgccc gccttggtca tgaacgttat
     2761 ctggcggagc aggtgcggat aaccggcaac agtacagacg ccacgctggc acctgctcag
     2821 gttctgacta tcaccgacag tcttccgccg acgttgcctg cgctgctgcg caatccggtg
     2881 ttgcttacct gtgtcggttt ttctgcaagt cgcaaggatg ccctgcaggt tgtactgaag
     2941 ggcgttcctt acagtgaagt tatctgctgg cgccctcctt tactgccgcg tccgaaagtt
     3001 accggtacca tgacggcccg tgtgaccagt gcaaaagaag gggatatcta cgcctggcag
     3061 gatgcatcag gtatgtaccg ggtgaaattt gatgctgacc gtgatgataa aaatccggga
     3121 caggaaagca tgccggtacg tctggccaaa ccctacagtg gtgatgctta tggttttcat
     3181 tttccgctga ttcagggaac ggaagtggcc attgcctttg aggaaggtga tccggatcgc
     3241 ccctatatcg cgcatgccct gcatgattca cgtcatgttg atcacgttac cgataagaac
     3301 ggtacgcgca atgttatccg tacgccggca aataacaagt tgcggatgga ggacaaacgc
     3361 ggtgaggagc atatcaagat cagtaccgag tttggcggta agacacaact taatcttggt
     3421 cataacgtta atgcctcccg gaaactcagg ggagaaggcg ctgagctgag gactgatgac
     3481 tggataagta tccggggagg aaaaggaata tttatcagtg cagatatgca gcctcaggca
     3541 caaggaaaga tgctggatat gaatgaagct atccgccaac tggagcaggc attatctctg
     3601 gcccgaagta tggcgaaggc cgcaactgct gcaaatgcta ctcagggaga tataagttgt
     3661 cagcaacggt tgaattcatc gctaacagat ctcactgcgc cgggaatgct gttgcatgct
     3721 cctgatggga ttggcatggt tagtgcacgg gcgttacgaa ttgcttcggg tagtgaaagc
     3781 gtgggcataa tgtcgggaga taacactgat attagtgccg ggcagtcatt tacagttgta
     3841 gcagaaggtg ctgttagcct gttgagcaga aaccagggga tgcaactgtt agcagcaaaa
     3901 gggcgggtaa atattcaggc tcaaagtgac gatttgtcaa tgagttccca acagaatctt
     3961 gatattcaaa gttctgaggg caaagtgact gtttctgcta atcaggaact gatactggca
     4021 tgtggtggag cttatatcaa actaagcggt ggaaatattg agctgggatg cccgggacag
     4081 atcctgttaa aaagcacgaa tatgcagaag atgggaccaa caagtcttga tattgcgtct
     4141 gttgaaatgc cgcgagggtt tggtggcggt tttattctta ccgatgaagc gggagtgccg
     4201 caaccttcga cgccttatcg attaacaacg gctgaaggtg atattctaca agggatcacc
     4261 gatgaaaatg gtaaaacagc gccagtcaac acctccattc cgtcagtagt aaaagttgag
     4321 tttgggaagg taaaaattca tggagaaaca gaatgacaaa atatcaggga tatgacgtaa
     4381 cagatgccac ccataaaacc agtattcata atgactggaa agttgttgtt gcaaagaaaa
     4441 aaccggcgcg gggtgtgact ttaaccattg gtatattttt cgatggtacc ggaaacaatc
     4501 gggagaatac agcatctcgc ctgatgaaat ttaatgagtg tagtgcagcc agacaggggg
     4561 taaatcaaaa agatgcacaa agctgtgagg actttcttga agaaattaac aagaacagta
     4621 tctcgaatgg cagttatcga ggatattatt ccaatattca ctggctaaat tcactttacg
     4681 tagcagatca gactttgttg gaaagtcagg ttgaagcaca aattcagact tacatttccg
     4741 ggatagggac tgcggctggt gaagcagatt cagtcattgg catgggatta ggcaccagta
     4801 ttctggatat atttgaaggt gtggtgacga aaaccgacga ggcaatggaa cgaatcaccc
     4861 aggcgttatc agaatttatg gggttcaatt taaatcctga tttttgtatc gcaaaaatcc
     4921 agtttgatgt ttttggtttt agccggggag cagcggcggc aaggcacttt gccaatcggg
     4981 ttatggaaca ggatcctgct atagccagag ctattgctaa agggctcaga ggcgattttt
     5041 atgatggtaa accttctggt gaagtccgtt ttctggggct ttttgatact gtcgcagcta
     5101 ttggtggaat cagtaatttc ttcgatatta atggacgtag caacccggga gtaaagctgg
     5161 aattgcgtcc ttcagtcgtg aagaaagttt tccagattac tgcaatgaat gaataccgat
     5221 ataatttcag cctgaacagt attaaaggta tgtggcccga actggcttta ccaggggcgc
     5281 attcagatat tggaggtggt tacaatccgg taggttcacc tctgcaggaa aatgaaagtc
     5341 tgtttctgag ttgcccggaa tttgaaattg tcagtgacga taccagagaa acggatacgc
     5401 gagtataccg taatgcagag caagccagaa aaatgttgat gactctgcca gccttaaaac
     5461 atattttgcc tcatggaaaa ctaaccacca aaattcgttc cgtaggtgtg aacaacagca
     5521 atcaacgtcg ggctggtgtg atacaaaagc aggtgggagc cgcagtattt tttgagcgta
     5581 tggcagtacc taatgactgg gctaatgttt gcctgcgtgt gatgctggat gctgctcagg
     5641 aagcaggggt attgtttgaa ccaattcgac aaacaaatac tgagttacaa cttccatctg
     5701 aactaatatt tcttgctgac aaagccattg ctcagggaaa agcggtacgt ttggggcaag
     5761 agcctcaggc ttttactgag gaagagttgt atattatcgg aaaatacacc cattgttctg
     5821 ccaactggaa tattgagtct gatggtaatt tatgggtgga tcctacaacg ggagagattt
     5881 ttatccatcg ctttggtcct aaaggtaata aggcgttcgt ttttccgaat aaaccaaatg
     5941 atcgctggat tcgttctgta tggtacatgg atgatcagca gcgattgaat gataacgcag
     6001 taaaaaatac taaagttatg atgtctggtg tttaacacca cagggtggca ata
//
LOCUS       DAZGTJ010000172         6007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000172 DAZGTJ010000000
VERSION     DAZGTJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.172"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            137..1555
                     /gene="araE"
                     /locus_tag="ACHK8E_004920"
     CDS             137..1555
                     /gene="araE"
                     /locus_tag="ACHK8E_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP0347050.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            1684..2376
                     /gene="ygeA"
                     /locus_tag="ACHK8E_004921"
     CDS             1684..2376
                     /gene="ygeA"
                     /locus_tag="ACHK8E_004921"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP0347051.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(2363..3298)
                     /gene="lysR"
                     /locus_tag="ACHK8E_004922"
     CDS             complement(2363..3298)
                     /gene="lysR"
                     /locus_tag="ACHK8E_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP0347052.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            3420..4682
                     /gene="lysA"
                     /locus_tag="ACHK8E_004923"
     CDS             3420..4682
                     /gene="lysA"
                     /locus_tag="ACHK8E_004923"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP0347053.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            complement(4689..5720)
                     /gene="galR"
                     /locus_tag="ACHK8E_004924"
     CDS             complement(4689..5720)
                     /gene="galR"
                     /locus_tag="ACHK8E_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP0347054.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
BASE COUNT         1394 a         1432 c         1651 g         1530 t
ORIGIN      
        1 ttgaaaattg gaatatccat cacataacga catgtcgcag caatttaatc catatttatg
       61 ctatttccga cctgacacct gtgtgaattg ttcacgtatt ttttcactat gtcttactct
      121 ctgctggcag gaaaaaatgg ttactatcaa tacggaatct gctttaacgc cacgttcttt
      181 gcgggatacg cggcgtatga atatgtttgt ttcggtagct gctgcggtcg caggattgtt
      241 atttggtctt gatatcggcg taatcgccgg agcgttgccg ttcattaccg atcactttgt
      301 gctgaccagc cgtttgcagg aatgggtggt cagtagcatg atgctcggcg cagcaattgg
      361 tgcgctgttt aatgggtggc tgtcgttccg cctggggcgt aaatacagcc tgatggcggg
      421 ggctatcctt tttgtgctcg gttcgatagg gtccgcattt gcgaccagcg tagagatgtt
      481 aatcgccgct cgtgtggtgc tgggcattgc tgtcgggatc gcgtcttaca ccgctcctct
      541 gtatctttct gaaatggcaa gtgaaaacgt tcgcggtaag atgatcagta tgtaccagtt
      601 gatggtcaca ctcggcatcg tgctggcgtt tttatccgat acagcgttca gttatagcgg
      661 taactggcgc gcaatgttgg gggttcttgc tttaccagca gtcctgctga ttattctggt
      721 ggtcttcctg ccaaatagcc cgcgctggct ggcggaaaaa gggcgtcata ttgaggcgga
      781 agaagtgttg cgtatgctgc gcgatacgtc ggaaaaagcg cgagaagaac tcaacgaaat
      841 tcgtgaaagc ctgaagttaa aacagggcgg ttgggcactg tttaagatca accgtaacgt
      901 ccgtcgtgct gtgtttctcg gtatgttgtt gcaggcgatg cagcagttta ccggtatgaa
      961 catcatcatg tactacgcgc cacgtatctt caaaatggcg ggctttacga ccacagaaca
     1021 acagatgatt gcgactctgg tcgtggggct gacctttatg ttcgctacct tcattgcggt
     1081 ctttacggta gataaagcag ggcgtaagcc ggctctgaaa attggtttca gcgtgatggc
     1141 gttaggcact ctggtgctgg gttactgcct gatgcagttt gataacggta cggcttccag
     1201 tggcttgtcc tggctctctg ttggcatgac gatgatgtgt attgccggtt atgcgatgag
     1261 cgccgcgcca gtggtgtgga tcttgtgctc tgaaattcag ccgctgaaat gccgcgattt
     1321 tggcatcacc tgctcaacga cgacaaactg ggtgtcgaat atgattatcg gcgcgacctt
     1381 cctgacactg cttgatagca ttggcgctgc cggtacgttc tggctctaca ctgcgctgaa
     1441 cattgcgttt gtgggcatca ctttctggct cattccggaa accaaaaatg tcacgctgga
     1501 acatatcgag cgcaaactga tggcaggcga aaagttgaga aatatcggcg tctgatttca
     1561 cgggccggat gtgctgtaca tccggccctt ttttcgttaa tagagattag gcacctggcc
     1621 gttgaggcgt ttgtctcgtt ccttattcag ccttgttgcg gtaacacaca tcaggagaga
     1681 ggaatgaaaa caattggttt gctgggagga atgagctggg aatccactat tccttactat
     1741 cgtctgataa atgaaggcat taaacagcgg cttggtgggc ttcactctgc gcaagtgctg
     1801 ctacatagcg tcgattttca tgaaatagaa gagtgccagc gtcgcggtga gtgggacaaa
     1861 accggggata ttctggctga ggcggcgctt ggcttacagc gggcgggcgc agaaggtatt
     1921 gtgttatgca ccaatacgat gcataaagtg gcggatgcca ttgagtctcg ttgctctctg
     1981 cctttcttac acatagcgga tgccactgga cgtgcaatta tccgggctgg aatgactcgt
     2041 gtggcgctgc tgggtacgcg ttacaccatg gaacaggatt tttatcgcgg gcggctgacg
     2101 gaacaatttt ccatcaactg tctcattcct gaagcggata aacgggcgaa aattaatcag
     2161 attatttttg aagaactgtg tctggggcaa tttaccgaag cgtcacgcac ttattatgcg
     2221 caagtgattg ctcgccttgc agaacagggc gcacagggtg tcatttttgg ctgtacggaa
     2281 attggtttac tggtgccaga agagcgcagt gttctgcctg tgtttgatac tgcggcgatc
     2341 catgccgagg atgctgtcgc ttttatgctg tcgtagctga cgacaaaata gcgtcaagag
     2401 aagtgaccag tttcggtaat cccgcttgta aatgctcact aaacgcctga accagcgctg
     2461 atgacgggcg gtgcaggggg cggatcaggc taacggtgaa cggaaccgca atactgaacc
     2521 gccgtaccac taaaccgctt gccgcataat ccagtgcggt gagcgggtta accaccgaaa
     2581 cacctacccc cgcccgtacc attgcgcaga ctgacgcggc gctgtgggtt tctacgatca
     2641 tgcgtcgttt aacctgattc tcagtaaaca attgatccag caactggcga tagctgtcag
     2701 tacgggaaag gctgatgtag ttttcaccgt gaaaatcatc cggcgttaat acctttttta
     2761 ccgccagcgg atggcccgga ggtaacacac acacttcatc taaagagagt aattcggtac
     2821 gttctgttcc cgcaggcgta tggagtgtct ccgtgagtcc taaatcatga cgctgggccg
     2881 agagccactc ttcaagtagc ggtgattcct ggggcacgat atttaagctg acatcgggat
     2941 aacgtgccag aaagggttgc aggagctgcg gtaaaaaaga ttgcgaaaag accggcaggc
     3001 aggcaataga cagttctccc tggcgaaact cgcgcagact ttctgcggcg ctgacaatgc
     3061 gatccagtcc gtaccaggat cgttgcactt cttcaaacag acgcagtcct tgcacggtag
     3121 gatgcaatcg tccacgtacg cgctcaaaca atttcagccc gatcaccttc tcaaagcgcg
     3181 ccagttcgcg gctgacagtt ggctgtgagg tgtgtagcag gtgtgccgcc tcagtcaggc
     3241 ttccggcggt cattaccgca tgaaaaattt caatatgacg taagttaacg gcggccatta
     3301 gcgctctctc gcaatccggt aatccatatc atttttgcat agactcgaca taaatcgata
     3361 ttttttattc tttttatgat gtggcgtaat cataaaaaag cacttatctg gagtttgtta
     3421 tgccacattc actgttcagc actgataccg atctcaccgc cgaaaatctg ctgcgtttgc
     3481 ccgcagaatt tggctgcccg gtgtgggtct acgatgcgca aattattcgt cggcagattg
     3541 cagcgctgaa acagtttgat gtggtgcgct tcgcacagaa agcctgttcc aatattcata
     3601 ttttgcgctt aatgcgtgag cagggcgtga aagtggattc cgtctcgtta ggcgaaatag
     3661 agcgtgcact ggcggcgggt tacaatccgc aaacgcaccc tgatgatatt gtttttacgg
     3721 cagatgttat cgatcaggcg acgcttgaac gcgtcagtga attgcaaatt ccggtgaatg
     3781 cgggttctgt tgatatgctc gaccaactgg gtcaggtttc gccagggcat cgggtatggc
     3841 tgcgtgttaa tccggggttt ggtcacgggc atagccaaaa aaccaatacc ggtggcgaaa
     3901 acagcaagca cggtatctgg tacaccgatc tgcccgccgc actggacgtg atacaacgtc
     3961 atcatctaca gctggtcggc attcacatgc acattggttc tggcgtcgat tatgcccatc
     4021 tggaacaggt atgtggtgct atggtgcgtc aggtcctcga attcggtcag gatttacagg
     4081 ctatttctgc gggcggtggg ctttctgttc cttatcaaca gggtgaagag gcggttgata
     4141 ccgagcatta ttatggtctg tggaatgccg cgcgtcagca aatcgcccgc catttgggcc
     4201 accctgtgaa actggaaatt gaaccgggtc gctttctggt agcgcagtct ggcgtattaa
     4261 ttactcaagt gcggagcgtc aaacaaatgg gtagccgcca ctttgtgctg gttgatgccg
     4321 ggttcaacga tctgatgcgc ccggcaatgt acggtagtta ccaccatatc agtgccctgg
     4381 cagctgatgg tcgttctctg gaacacgcac caacggtgga aaccgtcgtc gccgggccgt
     4441 tatgtgaatc gggcgatgtc tttacccagc aggaaggggg aaatgtcgaa acccgcgcct
     4501 tgccggaagt gaaggcaggt gattatctgg tactgcatga tacaggggca tatggcgcat
     4561 caatgtcatc caactacaac agccgtccgc tgttaccaga agttctgttt gataatggtc
     4621 aggcgcggtt gattcgccgt cgccagacca ttgaagaatt actggcgctg gaattgcttt
     4681 aactgcggtt agtcgctggt tgcatgatga cttgcctcca gcgacggagt tgacactgaa
     4741 tggcgacgta ccagcgtcgg actaaagaca ttagtgattt ccgggagagg gcgattatcc
     4801 gccagcgcca aagccagttc ggcagcctgg gtcgccatcg tcacgattgg gtaacgcacg
     4861 gtggtcagac gcgggcgcac atagcgtgac accagcacat catcaaagcc aattaacgaa
     4921 atctcacccg gtacatcaat accattatca ttgagcacgc ccatcgcgcc cgccgccatt
     4981 gaatcgttat aacaggctac cgcagtgaaa tttctgcctc gtcccaaaag ctcggtcatt
     5041 gcctgttcgc cgccgctttc gtctggttcg ccaaatgtca ccagccggtc attggccgga
     5101 ataccacttt cagcaagggc atcgtaatac ccttgcagac gatcttcggc gtcagaaata
     5161 gagtggttag agcacagata accaatgcgg gtatgacctt gctgaattaa atgacgcgta
     5221 gccagccagg caccgtaacg atcgtccaga gcaatacaac ggttttcaaa gccaggcagg
     5281 atacggttga tcagcaccat accgggcatt tgtttcatta atgaggctaa atcggcatcc
     5341 gggatcattt tggcatggac gaccaacgca gcacagcgat ggcggatcag ttgctcaatg
     5401 gcctgacgct ctttttgttc gttgtggtaa ccgttgccaa tcaataaaaa attaccagtg
     5461 tgataagcca cctgttcgac cgctttcacc attgcaccga aaaacggatc ggaaacatca
     5521 ccaacgacca gaccgaccgt ttcagtggtc tgctgcgcca gcgcacgggc gttggcgttc
     5581 gggtgatagc taagagactc cattgcacta tgcacagcaa gccgggaagc ttcgctggct
     5641 ttgggtgaat tattaatgac gcgggaaacg gtggcgactg aaacgcctgc cagtcgggct
     5701 acatccttta tggtcgccat gaaaatacct tagtgggtaa acgcttacat tctttcagtg
     5761 ttacggaaaa cgcccgttgg ttcaaggggc ggcgtgtttt cattgtggga atgtgagcca
     5821 aacgcaaata agtaagacaa aaaaagagat tgcaaacctt tggttacact ttgcgaaacg
     5881 ctgttgcgat tgaccgctgg tggcgtttgg cttcaggttg ctaaagtggt gatcccagag
     5941 gtattgatag gtgaagtcaa cttcgggttg agcacatgaa ttacaccagc ctgcgcagat
     6001 gcgcagg
//
LOCUS       DAZGTJ010000173         5901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000173 DAZGTJ010000000
VERSION     DAZGTJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.173"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..1058
                     /locus_tag="ACHK8E_004925"
     CDS             126..1058
                     /locus_tag="ACHK8E_004925"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0347055.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1099..2529)
                     /locus_tag="ACHK8E_004926"
     CDS             complement(1099..2529)
                     /locus_tag="ACHK8E_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFP0347056.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(2683..3309)
                     /gene="dsbA"
                     /locus_tag="ACHK8E_004927"
     CDS             complement(2683..3309)
                     /gene="dsbA"
                     /locus_tag="ACHK8E_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP0347057.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(3326..4312)
                     /gene="srkA"
                     /locus_tag="ACHK8E_004928"
     CDS             complement(3326..4312)
                     /gene="srkA"
                     /locus_tag="ACHK8E_004928"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP0347058.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(4389..4658)
                     /locus_tag="ACHK8E_004929"
     CDS             complement(4389..4658)
                     /locus_tag="ACHK8E_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP0347059.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            4728..5312
                     /gene="mobA"
                     /locus_tag="ACHK8E_004930"
     CDS             4728..5312
                     /gene="mobA"
                     /locus_tag="ACHK8E_004930"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP0347060.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            5294..5821
                     /gene="mobB"
                     /locus_tag="ACHK8E_004931"
     CDS             5294..5821
                     /gene="mobB"
                     /locus_tag="ACHK8E_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP0347061.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
BASE COUNT         1565 a         1362 c         1325 g         1649 t
ORIGIN      
        1 aattgtgata atgggatttt ttctaatgtg gatatatatg aattattttt ctccttaagg
       61 atcatccgtt atttggggcg ttttctgcta aaatcggcgc cttgcgcttc tttagcgcac
      121 tgtctatggc taaccttttg aataaattca ttatgacgag aatactcgct gcgataaccc
      181 ttttgctgag tatcgtattg accattttgg tcactatttt ttgctccgtc ccgatcatca
      241 ttgccgggat tgtaaaacta ttgctacctg tgccagtcat ttggcgaaag gtttcgcgtt
      301 tttgtgattt tatgatgtat tgctggtgtg aaagtctggc ggtattactg catctgaacc
      361 cacacctgca atgggaagtt cacgggctgg aagggttaag taagaagaac tggtatctgc
      421 ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact gtgcgtgctg tttcgtaagc
      481 acattccgat gaataaatat ttcctcaagc agcagcttgc ctgggtgcca tttcttggcc
      541 tggcatgctg ggcgctggat atgccgttta tgaagcgtta ttcccgcgct tatttattac
      601 gtcatccgga acgacgtggt aaagatgttg aaaccacccg gcgttcttgt gaaaagtttc
      661 gcctgcaccc caccaccatt gtcaatttcg ttgaaggctc ccgtttcacg caagaaaaac
      721 atcagcaaac gcactccact tttcaaaact tgttgccacc aaaggcggca ggcattgcga
      781 tggcgctaaa tgtactgggt aaacaattcg ataaactgct taatgttacg ctgtgttatc
      841 cggacaataa ccgtcagccg ttcttcgata tgttaagcgg taaactgacg cgaattgtcg
      901 tacatgtgga tttgcagcct atttccgatg agttacatgg cgactacatc aatgacaaaa
      961 gctttaagcg ccattttcag caatggctga actcactgtg gcaggaaaaa gaccggcttc
     1021 tgacgtcgtt gatgtcatca cagcggcagg acaagtaaat tttgctcgcc tctgaatgag
     1081 cagaggcgag tgagtatttt aatgaacaaa acgtccggca cgagacataa attcttcttc
     1141 gctcatctcc tgtccgttaa aaacaacttt acccggtgta taacgcagtt gcagcgaggc
     1201 ggtattgttg tcaatcgtga ttaactgaaa catctgacct aatgtcatca tcccgctgat
     1261 ttgtttatca gcccgctttt gcgctttttc agcatccatt ccttcagata aattaagctg
     1321 ttttgcggtt tctgtgacaa cattaagcgg taacttcaca tcaaaaatga gcgatttaat
     1381 atctttgttt gtggatgatg aggatttggc tgggtcggca atactgatat ccagattggc
     1441 atttagttcg ccgagtgcat tcttccatct taccggttgt ttaatggtcg gctcactttt
     1501 ttgtaataac ggtaaatact ctttgaacaa tgcagcattc acttcttgca gagcaacttc
     1561 atcgtttgct aactcagggt gtgcagcaag ctgcttctgc atcgcaatgt tatactggat
     1621 gacaaattgg cgcaccgctg agggatcaat agattcagca atcaatgacc actcaccact
     1681 accgagttgc tgattccctt ttgttaactt atcaagttcg taggtcagat tgacattgat
     1741 ataatctttg tctttaacgc gatccagcct ggtttttgca acgaaagcat cgatttgggc
     1801 aacgtcttct ccctgattga taatgttaat ctgattgaat ttcgactcgc tttcgccaac
     1861 cggaaatttc ttttcttcta atcgcgttag agaatcaatg gtaaaacttt tcgccgttac
     1921 tttattcaat ggcgataatt ccagaacaag atcttcgact ttgccttcaa tatccagatt
     1981 agacaatgaa ctaccatcac cattaaaagt aaactggccc tcaccaaaag aaaactttcc
     2041 atattttgca ggggcaacat ctaatgtcgt ggagaatgag ccaccaaacg cgaatcgcaa
     2101 tgtcgcttcc ataggagatt tattttttgc ggcgataaat agcggttgtg tcagttcatt
     2161 atccactaat ttcacttttg ccgctgccag tgcggggatg acattaccac gcattaacat
     2221 tgtgatgggt aacggaccgt gctcaacatc cacatcaaac gcaactttct ggccaggttt
     2281 gatattgaga tcaggtgcac cgttatcaaa tgtcatttgc atttgaaaac gagaattaaa
     2341 aaagcctttg tcaaaatttt tataacttaa cgtcatatcg taggcatgtt cacctttctt
     2401 tttagcatcg ttaaaatctt taataaactt ttcgatacca ggctgaattt gcgtacctgt
     2461 gtaccatgtg ccaccacccc agattacggc taacgcaaca ataacacctg tagctgactt
     2521 acgtatcata taaatattcc ctgataaaac agcaaaactt tgaatatcca cttatgctga
     2581 aataatgtcc agcggcagga cgcattatca gaatgtattt atatttatta aataataaaa
     2641 aagcccgtga atattcacgg gctttatgta atttacattg aattattttt tctcggacag
     2701 atatttcaca gtatcagcat actgctgaac aaaaacatcc atattgctgg tatccatacc
     2761 ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacgccac gcaattgcac
     2821 gtcagctgca gctttttcct gctgagcgac cagggatttc accacgaagc tgttccacgc
     2881 tgcgtcgtac tcttcacctt taatacctgc gttgataaat acatcgcgga tatcagatgc
     2941 tgaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtga ctttgtcttc
     3001 cacgcccagt gccatcgcca ctgcccatgc ctgagtcaga tctttgccca ggtcaccccc
     3061 catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt tcttcacgtt
     3121 atcagaaata tgcagaactt cttcaaactg atagcagtgc gggcagaaga aagagaaaaa
     3181 ctccagcact tgcggcgcgc cagcaactgg tttttccagg gtagtgtact gtttaccatc
     3241 ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg ccagccaaat
     3301 ctttttcatg atctactctc tccgattaat acataggtgt taattgcaaa gggggttctt
     3361 gtagaacttt tgcctgttct ataaaagtcg ccgtctgtcg cagccagtaa tcttccccgg
     3421 ttaaccacgg gaaatttttc gggaacgcgg gatcagccca acgccgcatt agccaggcaa
     3481 gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca gcggtgtcga
     3541 actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc tgctcggctt
     3601 tatcgccatt gagcaacatc cacaaatcct gaatagccgg accattacgt gcatcatcca
     3661 gatcaacaaa cattggacca tcgcgccaga gaatattccc ggcgtggcag tctccatgta
     3721 gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc agctcatctg
     3781 tcgctttcag aaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc tcaaacagct
     3841 tgcgaggctc aatgagatat tcattcagac cgatggtcgg gcgatggata aaaagctgtt
     3901 tgcgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccatc tgatcgatat
     3961 tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa aatccctgat
     4021 gattcaataa agtctgaccg ttaaaggcca caggtgctgc gaccggaact tcatcattta
     4081 ccaactgcaa cgcaaattga tgttcttcga ggatttgatc gggtgtccaa cgttcaggac
     4141 gataaaattt gacgacaaaa cgtcgacgat cttcgtcctg aaattgatag acacggtttt
     4201 catagctgtt aagcggggta agaccggaat ccacccggat cccttgctca aacagagcat
     4261 ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc atcccatcat
     4321 ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt ggcaccgctt
     4381 acaagctttt actctttaat tacgccgcga gcgcgtaaca gcgccgtttt gaagtcttct
     4441 tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg cattttcagg
     4501 tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc tgactctttc
     4561 gccagtttct gcaaaaattg cagcaggtta aggtctggct ctttttgcca ggctggctgg
     4621 aggagttcaa taacttcatt cagacgttta catttcatag taatgctcct tactcttgag
     4681 acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg aatctgatga
     4741 cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc ggcgtagata
     4801 aaggattgct tgaattaaac ggcaaaccat tatggcaaca tgtcgctgac gcgcttatga
     4861 cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat caggcaagcg
     4921 gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca ggaatgcttt
     4981 cagtaatgca gcaggaagcg ggtgagtggt ttttgttttg tccgtgcgat acgccttaca
     5041 ttccccatga tttagccgcc cggcttaatc atcagcgcaa agatgcgcct gtcgtgtgga
     5101 tccacgacgg tgaacgcgat cacccgacta ttgctctggt aaaccgcgca attgagcctt
     5161 tattactgga atatctgcaa gcaggagaac gccgggttat ggcatttatg cgtctggctg
     5221 gcggtcatgc ggttgatttc agcgatcgta aagaggcatt tataaacgtg aatacgccag
     5281 aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg ccgcgtggag
     5341 tggtactgga aaaactacac tgctgaaaaa actgatcccg gtattatgcg ccagagggat
     5401 ccgtccggga ctgattaagc atacgcacca tgatatggat gttgataaac cgggcaaaga
     5461 tagctatgag ttgcgcaagg ctggcgcggc acaaaccatc gttgccagcc agcaacgatg
     5521 ggccttgatg acggaaacac cagacgaaga agagctggat ctacattttc tcgcaagtcg
     5581 aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg aagagatcgc
     5641 aaagattgtg ctgtttcgcg atggagccgg acatcgaccg gaagaattag tgatagacag
     5701 acatgttatt gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcgt taatggatat
     5761 taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaagc aggacggata
     5821 agcaaaagta ggctggataa gacgcgccag cgtcgcatcc ggtatttatt ctgctccagc
     5881 gcaaaaaggc catcctgacg g
//
LOCUS       DAZGTJ010000174         5856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000174 DAZGTJ010000000
VERSION     DAZGTJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.174"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            506..731
                     /gene="ykgL"
                     /locus_tag="ACHK8E_004932"
                     /pseudo
     CDS             506..731
                     /gene="ykgL"
                     /locus_tag="ACHK8E_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(766..906)
                     /gene="ykgO"
                     /locus_tag="ACHK8E_004933"
     CDS             complement(766..906)
                     /gene="ykgO"
                     /locus_tag="ACHK8E_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP0347062.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(906..1169)
                     /gene="ykgM"
                     /locus_tag="ACHK8E_004934"
     CDS             complement(906..1169)
                     /gene="ykgM"
                     /locus_tag="ACHK8E_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0347063.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1534..1635)
                     /gene="ykgR"
                     /locus_tag="ACHK8E_004935"
     CDS             complement(1534..1635)
                     /gene="ykgR"
                     /locus_tag="ACHK8E_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP0347064.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2158..2358)
                     /locus_tag="ACHK8E_004936"
                     /pseudo
     CDS             complement(2158..2358)
                     /locus_tag="ACHK8E_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
     gene            2718..3611
                     /locus_tag="ACHK8E_004937"
     CDS             2718..3611
                     /locus_tag="ACHK8E_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0347065.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3642..4631)
                     /locus_tag="ACHK8E_004938"
     CDS             complement(3642..4631)
                     /locus_tag="ACHK8E_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0347066.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(4658..5509)
                     /locus_tag="ACHK8E_004939"
     CDS             complement(4658..5509)
                     /locus_tag="ACHK8E_004939"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0347067.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT         1570 a         1248 c         1371 g         1667 t
ORIGIN      
        1 taacgttcaa ttcttacttg cattttatat tagccaggaa ctttgccctg aggtgttagg
       61 aatagtctta atgtgttgtg taatggacta gtaaaaaaac aagtttaaat gataagaaat
      121 ggaatataaa actatcataa gtcataatta aagatgattt gccaatcagt aaggtgtttt
      181 aatggattaa catgccgcac aaaatggttg tgggtgttca tcccaataag tgatgaaaaa
      241 ttaaacaggt gtaagtacgt catctgtccg gtaagccccc caagagccaa attaagtgaa
      301 gttaaacgaa gactgccagt tgtcacagga acgatggcct ctttgccagg gcactgcatt
      361 cagtcatctg tacgcgttac tctaactggc actcgaacgt cggcgatggg gttaccgatg
      421 gatctggccg ttcctgtggc gtaaacgttt ttatgtcagt accaacagat ttaccacatt
      481 tatcatctta acagtttgag tgtaaatgca ggctgcgtcg caaagggagg aaactgagtg
      541 gcgtgttcag tctaaaatag agttgatgcc agcatacagg gcgcagctgg tcaacaagtc
      601 aacattaata taatggaata ttcggagaga aacgtcaggc agttgacctt aaattaacag
      661 gaagatacat ccccaggaaa attaatgctg gtgtaataaa tacaccaaca ttaatccggt
      721 atgattatta atcagacaaa tatcgttaat tcgtcgaatt ttgaatcaac gttttttctt
      781 acgaccctga acggccttaa aacgtggatt agatttacaa atcacataca gccgtccttt
      841 tcgcttcaca atctgacagt ctggatggcg ttcttttgcg gtacgcagag agttaaggac
      901 tttcatcatg cccccttttt cgtgctaaca aaacgaccaa aacgttgggt gaaccgtgca
      961 acatttcctt ctgatgccac tgttctcagc ttccctgtat agaacgggtg cgatttcgaa
     1021 gagacatcaa ttgttacgta cggatacgtt acgccatcca gctcaatctc acggtctgtt
     1081 ttgatagtcg agccgatttt aaagtactca tcaacgctgg tgtcgtggaa caccacagta
     1141 cgatactcag gatggatatt gggtttcatc atttttacct gttatgttat aacataaccg
     1201 taaagataca tgctcatccg gatcatttca acacccaatc actgtgacta tttgtgggat
     1261 gtggaatttc cgcaacgggg aagtctggca ttgttagcag ccattggcga cgctcccata
     1321 aacgcctgga tgagtgattg atgatggttt cgctgagagt atggattttt cgagaatgga
     1381 ggctatctcc actttactgg ccgatgattt attgcagcat gattgattac ctgacaatgt
     1441 gtattcctct cccacggata taataattac ttacaaaaaa gggagaggat gcatatttta
     1501 aatatcactg aagtgaacag tttattgccg ttattaatag aaatggagaa ataaataggc
     1561 gtattctaca attgcgacaa aaacaacgat attaatcagt ttatgactga tttgctgtac
     1621 tttattctct ttcattggta cttcctcgct ttaaaaaaga gtgcacttcg taagtgccct
     1681 tatataaata acgagtttgg tcaaccaatt ttttgacatg tatcacaaat ttgaatagat
     1741 gtattacatc ggctatcttt tattgcccca acgtcattga tatatgtcgc ctgaaatcag
     1801 ttacgggaat gagtctgatc tcaagactgg cccagtcctg gcgttgattg gcgctgagga
     1861 gcatattgca tctcatcgta acgtcgtatc tcctggggtg ttatacaaga tatcgttgta
     1921 ggtaaccagg gagaggaatt gagtctatta aaccgtcaac tatgccggat acatactgga
     1981 ttacactgca ggcacgcctt acgagagaac gtgccgcagt gacgggttaa ttatctgaaa
     2041 gaatttgcga ggctgtatcg gttactcatt gatttgatag ttttactctc gggagagtaa
     2101 tcgatattta atccattaac ggaaaccagc cagttccttt cgatgtctga atttccttgg
     2161 cgtgacatag gtatatcctg taattactaa accaatattt tgtgagcgac gtttataata
     2221 ttcgagttct tgctcagaga tggttccatc aggattagct gaccaggtcg tcataggagc
     2281 cataacgata cgattacatg gctttatttt ttctgtcagc ataaaaggag aaaacagtga
     2341 taggtgttta tttgtcatga caatacctca aatgtttatc tgattttgat gtgtatattt
     2401 aaactggtta gttgttacca gctttggaac caagattttt ggctttgttt ttatttataa
     2461 ttactgttag tgcttttatt gattttatgg tggagaaaag agtaaaaata gaaagttaat
     2521 gatgtgtgtc agaatatcgg taagaagaat ggcacaatcg tgagggtacg tttattcata
     2581 ataaatctcc tgaaagttta gaattatttt cgatacagcc agattatcat tgttactaat
     2641 aatgtggtta aaataatagc atctatgccc ccttcatgag tgtgtgtgaa gaggatgctt
     2701 ctacaaggaa atcgccaatg ctcaaagaaa acttcaacga actgcaaatc tttcttgtgg
     2761 tggcaagaga gcgaagtttt accaaagcag cgggcaaact cggcgtctct cagtctgcac
     2821 ttagccatgc aataaaggct ctggaggaaa ggctcaatat ccgcctcctg acacggacta
     2881 cccgcagtgt ggctcccaca gaagcggggg agagaatcat tgcctgcctt gaaccgcgta
     2941 ttgacgagct tgaacaggaa ctggaatcac ttattcaatt gaacggcacg ccctccggga
     3001 atattcggtt atctgccggg gaacatgccg cgcgaagtct ggtctggccg aagctaaaac
     3061 cttttctcag agagtatccc gaaatcaatg tcgaactggt ggtggataac ggttttgtcg
     3121 atattgttga aggccgtttt gatgccggga tacgactggg tgaaaatgtt gataaagata
     3181 tggttgcagt caggattgga ccagatatgc ggatggctgt ggtgggagct ccggcgtatt
     3241 ttgctgcaaa ccctgcgcct gaaacgcctc acgagctaca aaatcatcgc tgcatcaaca
     3301 tgcgtttgcc aactgccggt gggctttacc actgggagtt cgagcgagaa gggaaaccgc
     3361 ttcgcgttaa ggttgatggg caactgacgt gtagtccatt gccagaaaga attgatgccg
     3421 cgttatcagg gtttgggatc gcttgtgtcc ctgaagacat ggttcaggag tatatagagt
     3481 caggcaagct tattcaggtg ttacaggagt ggtgtccaac ttttcctggg tattatcttt
     3541 actaccccag ccgcaagcag catccgccag cttttgcgct gttgatcgat gcgcttcgct
     3601 acaccgaata acgggtcctt tatcctggac ccgtttgggt attagcggcc tacgcgtgcc
     3661 tgatgttcag gcgagtaacg ttcaccgacg attttaattg tttccagcgc ctcgcttatc
     3721 tgctgcgtat ctttctgcga aagaacaatg tcagcagcgg ccaggttttc ttccagccgg
     3781 tgcagtttag tggtgccagg gatgggaaca atccacggct tttgtgctaa tagccatgcc
     3841 agtgcgattt gtgcagacgt tacccctttc tccgcagcca gttcacctaa caaggtgacc
     3901 agtttttcat tggcttcaat ggattgtgct gcgaaacgcg gtacggtgct gcggtaatca
     3961 tctttgccga aagtggttcc cggcttaatc gcccctgtca ggaaaccttt acctagtggg
     4021 ctgaagggga caaaaccaat tcccagttcc tccagcaacg gcagaatttc ctgttcaggc
     4081 tcacgccacc acatggaata ttcgctttgc agggcggtga caggttgtac cgcatgtgca
     4141 cgacgaatgg tttgtgctcc tgcttcagac aggccgaaat gtttgacctt accttctgcg
     4201 atgaggtctt tcaccgttcc tgcaacatct tcaataggaa catctggatc aacacggtgt
     4261 tgatacagca ggtcaataac atctgttttc agacgacgta atgatccttc aacggcttca
     4321 cggatatgtt ccggacggct gtttaaaatc tgctgcttat tatcatcgcc gaaggtaaaa
     4381 ccgaatttag tggcaattac cacgcggtca cgaaacggtt ttaaggcttc gccgaccact
     4441 tcttcattca gatacggacc atacacttcg gcggtatcga agaaggtgac gccacgttca
     4501 accgcagcac gaataagttc gatagcctga cgggtatcgg tcgccgggcc gtagccgtgg
     4561 cttaagccca tgcaaccgag cccaagggcg gagacttcga gtccggattt acccagataa
     4621 cgtttttgca ttgaatgaat acctctttta tcgcggctta aacatcaagt ttgcggccag
     4681 tcagccattc caccattgcg ggatcgcggt gagagaagaa cgcgctggtt gcggtatcca
     4741 gggcggtaat ttgcagcata tcttcagggc tgagttcaaa atcgagaatg ttgatgttct
     4801 cttccatgcg ttcttttcgc actgattttg ccagtgaaac aatgcctcgt tggtagatcc
     4861 agcgcagaac aacctggccc acgcttttgc catacttctc gccaattgcc gtgagaacag
     4921 gatgctggaa cagaccattt ttcccttccg caaacggtgc ccaggcttct ggctgaatgc
     4981 cacgactttg catccaggga acggcctgta gctgctgatt gaaagggtta acttcaacct
     5041 ggtttaccgc cggaacgacg ttattaaaag cgataaggtc tgccagtcgg tcaggatgga
     5101 agttactgac gccaatagcg cgaattttac cggcctgttg cagctcttcc atggcacgcc
     5161 aggctccgtg gacatcgcca tatggctggt gaatcaagta caggtcaacg taatcaagtt
     5221 gcagtcgatt cagagagcgt tcgaactggg ctttggcccc ttcgtaactg gtatcctgca
     5281 accagagttt cgtggtgaca aagagttcgt tacgggcgat accgctttgt ttcagtgcgt
     5341 tcccgacctg gatttcattt tgataagagg cggcggtgtc aatcagtcga taaccgctgt
     5401 taatagcgtc aatgaccgct ctttcgcatt cggcggcatc ggtcatctga aatacaccaa
     5461 agcccaacag gggcatttca ataccgttgt tcagttttac tttttgcatg gtgttatcct
     5521 tccgttatcg ttcatgagaa gcataacgta aagggaagag ctcgattaga cggcaggatt
     5581 tgtcaggggt tatgaacaaa attcataaat ctgtttgagg gttgagatta gtcgtgcaag
     5641 ttcgatatcc ccggaattta cagtccgatg aataaaaatg aatgtccata aaaaatatat
     5701 tggtttatcc cagcgtatat acttacttcg acgtactccc aatagcagtt aattaaagac
     5761 gggtcattat tattcactct gaataatgaa ttattcactg cccgcagagt aagaaatata
     5821 acttaagtat ctatttaatg acttgcacaa aaagct
//
LOCUS       DAZGTJ010000175         5745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000175 DAZGTJ010000000
VERSION     DAZGTJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.175"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..112
                     /locus_tag="ACHK8E_004940"
                     /pseudo
     CDS             1..112
                     /locus_tag="ACHK8E_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            260..1279
                     /locus_tag="ACHK8E_004941"
     CDS             260..1279
                     /locus_tag="ACHK8E_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0347068.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2000..2215
                     /locus_tag="ACHK8E_004942"
                     /pseudo
     CDS             2000..2215
                     /locus_tag="ACHK8E_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2390..4360
                     /locus_tag="ACHK8E_004943"
     CDS             2390..4360
                     /locus_tag="ACHK8E_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFP0347069.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            4379..5170
                     /locus_tag="ACHK8E_004944"
     CDS             4379..5170
                     /locus_tag="ACHK8E_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFP0347070.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1760 a         1255 c         1353 g         1377 t
ORIGIN      
        1 tggaaaacag tttagacgag gaagagatgc atctgcgact ctgggactgg ttccccgaca
       61 gttcagcaca ggtgggaagc aggttcttct gggtataaca aaatttggag aaatgtaatg
      121 aacacatccc accccattaa gtcaggtgct ctgtgatgga tgaagggaaa tactggaagg
      181 tgtggagcct cgcagatttc cggcatcata gtgcccaata caggatgcat aaagctccct
      241 cagactggag actccacaaa tgaaatatac accggttggc gttgatatcg caaaacatct
      301 gattcaggtt catttcatcg acgagcacac aggtgaagtg attgataagc aactacgtcg
      361 tcaggatttt ctgatgttct tctgtaatcg ggaaccctgc ctgattggta tggaggcctg
      421 tagaggctct cagcactggg ggcgtgaact gacaaagctg gggcataaag tccggttgtt
      481 acaggcccac tttgttaagg cattcgtcat gggaaataag aatgatgtga tggatgcccg
      541 ggctatctgg atggctgttc agcagccagg taaggacatc gcggttaaaa cagaagaaca
      601 acagtcagtg ctggttttgc accgttcccg cagacaattg gttaagttcc gcaccatgca
      661 gattaattct ctgcacggca cgctcctaga atttggtaaa accattcaca aaggtcgggc
      721 agcgatggag cgagagctcc ctgaagcact ggagcggatg aaagagagac tgccgccgta
      781 tctcattact gtgctggaaa accagtacaa ccggctgaat gagctggact cgctgataga
      841 ggatattgaa aaaaagctta ccagcgtggc gagacagaat gaaacctgta agcggttgct
      901 ggatattccc ggcgttgggc cacttattgc aacggcagcg gtggccacca tgagggaagc
      961 atcagcgttt aaatcagggc gagagttcgc ggcttatgtt ggtctggtgc ccaaacagac
     1021 tggctcagga gggaaaatcc gcctgctgga gataagcaaa cgtggtaata catatctgcg
     1081 agccttgttt attcatagcg caagggcggc agcattactg cccaaagaac ctggtccgtg
     1141 gataaccgaa ctgaaaaaac gtcgtccaac cagcgtggca atcgtcgcca tggcaaacaa
     1201 actagcacga acagtatggg cgatagcagc tcatggaagt aaatacgaca agaatcatgt
     1261 cagtatcaga tcatattaat cgctgatacc gataaatatt aacttttaat gaaaggatga
     1321 atactgaaag gtaactctgg cggccatagt gatgacaacg acaggtaaga ccgggactca
     1381 ctaaacctga atattgtgtt gagctaaaag ctcgctggag aaatgaggtg tgagtcagcg
     1441 aattacatag gggctcgcag cttcatggct gcaaaaaagc cggatataaa gctgcaacct
     1501 acccgtcact gtcaaggcaa tggatgcctt gcaacgggat gtgttcatat aatcaatatg
     1561 tcagatctat gctgatacag ggtgccagag cggtattgtt cagagcctca gagaaaactg
     1621 attcacttcg tgtgtgggtt aattgtattc gggaaaagcg tggcttcaac caggcagttg
     1681 ttgctcttgc aaataaactc cttcggatcg cctgggcaat tatcgcccgc aatgagatgt
     1741 atttgcccgc agcaaaacaa aatcaataat ctacgtaaaa tcagtaggag taaagcttac
     1801 cctgaaattg caaagaatgc agaatgatga aaaacaggtc agaccgacgt tacgaaaacc
     1861 tgcaagatga gccaggccag taaggccgag taagcgataa ggtcggagcg cgcgaatgtc
     1921 tcatcaaggc cagaagcaat aacttcatga acaaaccgga tatacgaaag taaacctgtc
     1981 cctatcatag ttgctgtact cttgcaatac gggggaagac catctcatcg cttacgaaca
     2041 attcgtgaaa acgatgaaga aagactacat cgcgtgcatg ccgaaatcta atgtaagaac
     2101 ggcattgcat aatcttgcag tggagatcgg gtattacaat gaaaaccatc cgcatagtgc
     2161 gttaggttat ctcccaccaa gggaatatca acgtcagcga ataacgtcac cttagatgca
     2221 gtagttgtca ggaaatagtg actcaagggc aggcttacca tatacactct tagtggttcg
     2281 actgggtatt agaatagcta ttaacagatt agcactgaac actgcgtaat agtggttgca
     2341 caaacaaaat gttatgttat aacattgctg tattgacata tattttcgaa tgtatatgaa
     2401 cgtaatcaga actgtcattt gtacattaat catacttccg gtggggttac aggcagcgac
     2461 aagtcattct tctatggcta aagatacaat caccgttgtc gcgacaggaa atcagaacac
     2521 ggtatttgaa acgccgtcga tggtcagtgt catcacgaat gacacgccat ggagtcagaa
     2581 tgcggtcaca tcagccggta tgctgaaagg tgttgcgggt ctcagccaga ctggtgcagg
     2641 acggaccaat gggcagacct ttaatctacg cggctatgac aaaagcgggg tacttgttct
     2701 tgttgacggc gtccgtcaac tcagtgacat ggcaaaaagc agtggcactt atctggatcc
     2761 ggcactcatc aaacgcatcg aagttgtccg cggaccaaac tccagtctgt acggcagtgg
     2821 cgggctgggg ggggtggtgg acttcagaac tgccgatgct gcagattttc ttccccccgg
     2881 agagacaaat ggtgtaagtc tgtggggaaa tatcgccagt ggtgaccaca gcacaggctc
     2941 ggggctcacc tggttcggta aaaccggaaa aacagatgcg cttctttctg tcattatgcg
     3001 taaaagagga aatatctatc agagtgatgg tgagcgtgca cctaacaagg aaaaacctgc
     3061 agccctgttt gcgaaaggct ctgtcagtat aacagacagt aacaaagcag gtgccagctt
     3121 gcgtctctac cagaataaca ccactgaacc gggcaatccc actctgacac atggtgacaa
     3181 cggcctgcgt gacagaaaaa cagcacaaaa tgacgtgcag ttctggtacc agtactcccc
     3241 tgcggataac agcctcatca atgtaaagtc aacgttatat ctcagtgata tcactatcaa
     3301 gacaaacggt cacaacaaaa cagcagaatg gcgaaacaac agaacctccg gtgttaatgt
     3361 tgtcaacagg agccatactc tgatttttcc gggagcccat cagttaagtt atggcgccga
     3421 atattaccgt cagcagcaga agccggaagg ctctgccaca ctatatccgg aaggaaacat
     3481 tgactttaca tcgttgtatt tccaggatga aatgacaatg aaaagcaacc cggttaacat
     3541 tatcgtcggt tcccgttatg accggtacaa gagcttcaat ccccgagccg gaaaactgaa
     3601 agccgaacgc ctgtccccca gggcggcgat ttcagtctca ccaacagact ggctgatgat
     3661 gtacggctcc atatcctctg cattccgtgc ccccacaatg gcagaaatgt acagggatga
     3721 tgtacatttt taccgcaagg gtaaacccaa ttactgggta cctaacctta atctgaaacc
     3781 agaaaataac attacccgtg agattggcgc aggtattcaa ctggacggcc tgcttacaga
     3841 caatgaccgg ctgcagttga aaggcggata tttcggaacg gatgccagaa actatattgc
     3901 cacgcgcgtg gatatgaaac ggatgcgttc ttattcttat aatgtatccc gggcccgtat
     3961 ctggggatgg gatatgcagg gtaattacca gtctgattat tttgactgga tgctttctta
     4021 taaccgcacg gaaagtatgg atgccagtag cagggaatgg ctgggctccg gcaatcctga
     4081 cacactcatc agtgacatca gtatacctgt tggtcatgga ggagtttctg ccggatggcg
     4141 tgctgaactt tcggcagcag ccacgcatgt gaaaaaaggc gatccccatc aggctggcta
     4201 tgccgtacat tccttttcac tgtcttataa gcctgtaagt gttaaaagct ttgaggcatc
     4261 agtgactctg gataatgcct tcaacaagct tgccatgaat ggcaaaggtg tgccgctttc
     4321 aggcagaact gtcagtcttt atacccgtta tcagtggtaa cctcgcagga aataaatcat
     4381 gatgaaaaat acaggctata tcttagctct ttgtctgaca gcatcggagc atgccctggc
     4441 ccacgatgtc tggattaccg gtaaacaggc agagaacaac gttaccgcag agattggtta
     4501 tggtcataat ttcccctcaa agggcacaat tcctgacaga aggaatttct ttgaaaatcc
     4561 ccggctttat aacgggaaag agataataac gctgaagcca acgtccacgg attatgtcta
     4621 taaaactgag tctgcagcca acggtaatag ctacgttctg tcaacgaaaa tgaaaccggg
     4681 atactggtcg agaacctcgt caggatggaa gcctgtcagt cgggagggca gaaatgatgt
     4741 ggcatactgt gagtttgtga ctaaatatgc aaaatctttt attcctggtg aacagcagat
     4801 gccagcacaa ctctatcagt ctccaacagg ggatgagctt gaaatcattc cattatccga
     4861 tatcagtcgt ttcggtgaag atgtgaagct gaaagttctg tataaaacat ccccgcttgc
     4921 cggagctact ctggagcttg actctgtcag ttatctgaaa tcatcccgtc atacccatgc
     4981 agctgagcat aaacattcgg ctcataaagc agaacttacc tttgtaagta atgaggacgg
     5041 tatcatcact gtaccttccc ttcatgtcgg gcaatggctg gctaaagtca aaaataagaa
     5101 agtatttccg gacaagagtc tgtgtgatga aactgtcgat gtggcaacct taagcttctc
     5161 ccgaaattaa taatactatt cctgccctga tttatgggca gggtattatc aatacgagaa
     5221 tagcctaccg acatccgtgt aaaaaaccac ataatgattt ttcatatcca tttccagata
     5281 tacttatgta aagatcacct ccaaaaaagc gtaatagtaa taaaaatccc tgaagcaccc
     5341 tgttcaaacc agaaggaata cataacaaga caataaaatg tgaaatccat cacagcaaag
     5401 aactctttgt ttataatata atgaaaatta gctaatattc tttaacaaga actgttggtc
     5461 atataaaatt aaatattttt atgctaaatg gttccatacg tgaagttata tcagaataag
     5521 gagacatagc cagaaaactt tcttttcata aactctttaa tattcaaaag aaaatattat
     5581 acccagcatt atttatttga aaatcacatc gtttatcatg aggagcatat tatctcctcc
     5641 agcaccaaaa ataaaaacac aaaacaacct tacaacttat atattaacac aaagtagaga
     5701 aagaagaaca actcatctta aaacaagaaa aatatacaaa ttaaa
//
LOCUS       DAZGTJ010000176         5476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000176 DAZGTJ010000000
VERSION     DAZGTJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.176"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(81..818)
                     /gene="bamD"
                     /locus_tag="ACHK8E_004945"
     CDS             complement(81..818)
                     /gene="bamD"
                     /locus_tag="ACHK8E_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP0347071.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            953..1933
                     /gene="rluD"
                     /locus_tag="ACHK8E_004946"
     CDS             953..1933
                     /gene="rluD"
                     /locus_tag="ACHK8E_004946"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP0347072.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1930..2661
                     /gene="yfiH"
                     /locus_tag="ACHK8E_004947"
     CDS             1930..2661
                     /gene="yfiH"
                     /locus_tag="ACHK8E_004947"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP0347073.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2791..5364
                     /gene="clpB"
                     /locus_tag="ACHK8E_004948"
     CDS             2791..5364
                     /gene="clpB"
                     /locus_tag="ACHK8E_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP0347074.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1350 a         1310 c         1485 g         1331 t
ORIGIN      
        1 cagccactgg atcggtcaac tgatccttaa ctgatcggca ttacagctca agggctgccg
       61 ttttgtgttt caggtttctg ttatgtattg ctgctgtttg cggcgatgat tttcgctact
      121 ttttcagctt gcgcattcaa ctgcatctga cggtatgcat tttccatcag cggcagcgca
      181 tcacgcgtag cctgggtatc cgggtagtcg cgcaacatgc cttctacgcg gttaacgacg
      241 gcaacccatg caccacgttc tgtatagtac tcggcaacgg agtattcata tttcgccaga
      301 cgatctttca ggaataccag acgtttggtg gcatcggtgg tgtactgact gttcggatag
      361 ccgcgcacca gtttggaaaa gtcactaaac gcagctcgtg catgttgagg atcgcgatcg
      421 ctacggtcaa cgccaaagaa cccttgcagc gcactgtcat ccagcgccat attggtcagg
      481 ccacgcatgt acatgacata atcgatattc ggatgggtcg ggttaaggcg aataaaacga
      541 tcgatggcag cctgtgctaa cggcaaatcg gcgtttttat agtaggcgta gatgagatcc
      601 agctgcacct gctgcgaata cggaccaaac ggatagcgat tatctaacgc ttccagttgc
      661 gttattgcct gtctccagtt accgtcctgc agcttttgtt gtgcagtcgc gtaaatttca
      721 tttggcggat tatcaggtac ttcttccttt gaccccgagc aacccgccaa aaacaggctt
      781 agtgtggcgg ctgccaccag atatttcatg cgcgtcatga cgttttgact ttcctcaaaa
      841 tgtaatacgg gagattctct gttcctgctc ccggttaaga ccagctacaa tagcacacta
      901 tattaaacgg caaagccgta aaaccccaac gataaacgaa gaagcagtat atatggcaca
      961 acgagtacag ctcactgcaa cggtgtccga aaaccaactc ggtcaacgct tagatcaggc
     1021 tttggccgaa atgttcccgg attattcacg ttcgcgaata aaagaatgga tcctcgacca
     1081 gcgcgtgctg gttaacggca aagtttgtga taagccgaaa gaaaaagtat tgggtggcga
     1141 gcaggtggcc atcaacgctg agattgaaga agaagcgcgt tttgaaccgc aggatatccc
     1201 gctggatatc gtctatgaag atgaagatat catcgtcatt aataaaccac gcgacctggt
     1261 ggtacatcct ggcgcgggta acccggatgg tacggtactg aatgcgttgc ttcattatta
     1321 tccgcccatt gccgatgtac cgcgtgcggg catcgtccat cgtctggata aagacaccac
     1381 cggcctgatg gttgtggcaa aaaccgttcc ggctcagacg cgtttagtcg aatctttgca
     1441 acggcgtgaa attactcgtg agtatgaagc ggtggcaatt ggtcatatga ccgctggcgg
     1501 tacagtagat gaaccaatca gccgccaccc gaccaaacgt actcacatgg cggtgcatcc
     1561 gatgggtaaa ccggcggtca ctcactatcg catcatggaa cacttccgtg tgcacacgcg
     1621 tctgcggttg cgtctggaaa ccggacgtac gcaccagatc cgcgtgcata tggcccatat
     1681 cactcatccg ctggtgggcg atccggttta tggtggccgt ccgcgtccgc caaaaggtgc
     1741 ttcggaagcg tttatctcca cgctgcgtaa gtttgaccgc caggcgcttc atgcaaccat
     1801 gctgcgtctt tatcacccga tctccggtat cgaaatggaa tggcatgcgc ctattccaga
     1861 agatatggtg gagctgattg aggtgatgcg cgccgatttc gaagagcata aggatgaagt
     1921 ggactggtta tgagtaagct gattgtcccg cagtggccgc tgccaaaagg tgttgcggcc
     1981 tgtagctcca ctcgtatcgg cggcgtgagc ttgcccccgt atgattcgct caacctcggt
     2041 gctcattgtg gcgataaccc ggatcacgtt gaagagaatc gtaagcgact ttttgctgcg
     2101 ggcaatttgc cttcaaaacc ggtctggctt gagcaggtac acggcaaaga tgtgcttaag
     2161 cttactggcg aaccttatgc ctcaaaacga gcagatgcct cttatagcaa cacgccgggc
     2221 acggtttgcg cagtgatgac tgccgactgc ctccccgtgc tgttttgcaa tcgagcggga
     2281 acggaagtcg ccgccgctca tgctggctgg cgtggactgt gctcaggcgt gctggaagag
     2341 acggtttcct gttttgctga taatccggaa aatattctcg cctggttagg gccggcaatt
     2401 ggtccacgcg cgtttgaagt gggggcggag gttcgcgagg cgtttatggc agtagacgcc
     2461 aaagcgagta cagctttcat tcagcatggt gataagtatc tggcggatat ttatctcctt
     2521 gcccggcagc gtctggcgaa cgtcggtgtc gaacaaattt tcggcggtga ccgttgtaca
     2581 tatacggaaa atgagacttt cttctcttat cgtcgcgaca agaccaccgg tcgtatggca
     2641 agtttcattt ggctgatata acctaaagaa tcaagacgat ccggtacgcg tgattttctt
     2701 ttcacattaa tctggtcaat aaccttgaat aattgaggga tgacctcatt taatctccag
     2761 tagcaacttt gatccgttat gggaggagtt atgcgtctgg atcgtcttac taataaattc
     2821 cagcttgctc ttgccgatgc ccaatcactt gcactcgggc acgacaacca attcatcgaa
     2881 ccacttcatt taatgagcgc cctgctgaat caggaagggg gttcggttag tcctttatta
     2941 acatccgctg gcattaatgc tggccagttg cgcacagata tcaatcaggc attaaatcgt
     3001 ttaccacagg ttgaaggtac tggtggtgat gtccagccat cacaggatct ggtgcgcgtt
     3061 cttaatcttt gcgacaagct ggcgcaaaaa cgtggtgata actttatctc gtcagaactg
     3121 ttcgttctgg cggcacttga gtctcgcggc acgctggccg acatcctgaa agcagcaggg
     3181 gcgaccaccg ccaacattac tcaagcgatt gaacaaatgc gtggaggtga aagcgtgaac
     3241 gatcaaggtg ctgaagacca acgtcaggct ttgaaaaaat ataccatcga ccttaccgaa
     3301 cgagccgaac agggcaaact cgatccggtg attggtcgtg atgaagaaat tcgccgtacc
     3361 attcaggtgc tgcaacgtcg tactaaaaat aacccggtac tgattggtga acccggtgtc
     3421 ggtaaaactg ccatcgttga aggtctggcg cagcgtatta tcaacggcga agtgccggaa
     3481 gggttgaaag gccgccgcgt actggcgctg gatatgggcg cgctggtggc tggggcgaaa
     3541 tatcgcggtg agtttgaaga acgtttaaaa ggcgtgctta acgatcttgc caaacaggaa
     3601 ggcaacgtca tcctgtttat cgacgaatta cataccatgg tcggtgcggg taaagccgat
     3661 ggcgcaatgg acgccggaaa catgctgaaa ccggcgctgg cgcgtggtga attgcactgc
     3721 gtaggtgcca cgacgcttga cgaatatcgc cagtacatcg aaaaagatgc tgcgctggag
     3781 cgtcgtttcc agaaagtgtt tgttgccgag ccttctgttg aagacaccat tgctattctg
     3841 cgtggcttaa aagaacgtta cgaactgcac caccatgtgc aaattactga cccggcaatt
     3901 gttgcagcgg cgacgttgtc tcatcgctac attgctgacc gtcagctgcc agataaagcc
     3961 atcgacctga tcgatgaggc agcatccagc attcgtatgc agattgactc aaaaccagaa
     4021 gaactcgacc gactcgatcg ccgtatcatt cagctcaaac tggaacaaca ggcgttaatg
     4081 aaagagtctg atgaagccag taaaaaacgc ctggatatgc tcaacgaaga actgagcgac
     4141 aaagaacgtc agtactccga gttagaagaa gagtggaaag cagagaaagc atcgctttct
     4201 ggtacacaga ccattaaagc ggaactggaa caggcgaaaa tcgctattga acaggctcgc
     4261 cgtgtggggg acctggcgcg gatgtctgaa ctgcaatacg gcaaaatccc ggaactggaa
     4321 aagcaactgg aagccgcaac gcagctcgaa ggcaaaacta tgcgtctgtt gcgtaataaa
     4381 gtgaccgatg ccgaaattgc tgaagtgctg gcgcgttgga cggggattcc ggtttctcgc
     4441 atgatggaaa gcgagcgcga aaaactgctg cgtatggagc aagaactgca ccatcgcgtt
     4501 attggtcaga acgaagcggt tgatgcggta tctaacgcta ttcgtcgtag ccgtgcgggg
     4561 ctggcggatc caaatcgccc gattggttca ttcctgttcc tcggcccaac cggtgtgggg
     4621 aaaactgagc tttgtaaggc gctagcgaac tttatgtttg atagcgacga ggcgatggtc
     4681 cgtatcgata tgtccgagtt tatggagaaa cactcggtgt ctcgtctggt tggtgcgcct
     4741 ccgggatatg tcggttatga agaaggtggc tacctgaccg aagctgtgcg tcgtcgtccg
     4801 tattccgtca tcctgctgga tgaagtggaa aaagcgcatc cggatgtctt caacattctg
     4861 ttgcaggtac tggatgatgg gcgtctgact gacgggcagg ggagaacggt cgacttccgt
     4921 aatacggttg tcattatgac ctctaacctc ggttccgatt tgattcagga acgcttcggt
     4981 gaactggatt atgcgcacat gaaagagctg gtgctcggtg tggtaagcca taacttccgt
     5041 ccggaattca ttaaccgtat cgatgaagtg gtggtcttcc atccgctggg tgaacagcac
     5101 attgcgtcga ttgcgcagat tcagttgaaa cgtctgtaca aacgtctgga agaacgtggt
     5161 tatgaaatcc acatttctga cgaggcgctg aaactgctga gcgagaacgg ttacgatccg
     5221 gtctatggtg cacgtccgct gaaacgtgct atccagcagc agatcgaaaa cccgctggca
     5281 cagcaaatac tgtctggtga attggttccg ggtaaagtta ttcgcctgga agttaatgaa
     5341 gaccggattg tcgccgtcca gtaatgataa aacgagccct tcggggctcg tttttgtcta
     5401 taaattagac ggaaaagact atgtttaaga tgttttgcct gaaaaatgag cgaacgataa
     5461 agtttttata tttttt
//
LOCUS       DAZGTJ010000177         5471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000177 DAZGTJ010000000
VERSION     DAZGTJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.177"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            526..4617
                     /locus_tag="ACHK8E_004949"
     CDS             526..4617
                     /locus_tag="ACHK8E_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0347075.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTDRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMIDFS
                     SVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFS
                     KLITEAAPADINIFGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGT
                     PWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKWSLAGVTLHNYG
                     VNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQF
                     VMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFADDYTVTTSNDSSWSGGG
                     IIVNYGTTVKWGINGVFGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNND
                     STAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKDTEFSRLQAVDYGAA
                     IINSSIDKSLLTLNLSPLKKDEIAVSVKVLDMNAIFQGGHGTAGDLYKTTFYGPTQYY
                     LLKKPKFGSVLMGALKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNM
                     DIVIPELTGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAI
                     HAPSASATVNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSE
                     QAIGFSNVTPVITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGT
                     RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            5150..5470
                     /locus_tag="ACHK8E_004950"
                     /pseudo
     CDS             5150..5470
                     /locus_tag="ACHK8E_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1600 a         1138 c         1304 g         1429 t
ORIGIN      
        1 ctgtgtactc atccacgcca tccgtttttc agtctcagtg tgtgaataat aaattacact
       61 ttgaactgaa cgccagtcag gaccatgtcg tatttccggg tggatgagca tatatcagaa
      121 actacaataa ctacagataa cggttttcag taattctgtc ataacttgcc ggaatgcaat
      181 gtttttgtga catggttctt attttttata cacaggcagg ttttgtctgg atggaatgga
      241 taatgaactc ttatcattat aaggcggtaa cctctttttt atgtgattaa ctttcattat
      301 taccacttct gtttatcgat tcagctctgg agatacagaa aaattcagtg caggtgtata
      361 aatcatcacc atgcggaata cataaaatat gttaatattt gttttccgca ggaaaatctt
      421 aagtgtggac aggtatttat gataccattt aaaatagatt cagcactcac tctttccgga
      481 cagataatac atttcaatac catataatta tataacagga attatatgaa taaaatatac
      541 gcgcttaaat atagctccct tactggtggg cttatagctg tgtcagaatt aagtaagaag
      601 gtcacaggaa aaaccgatag aagattaatg acggtttccc tggtattatc agtgactctt
      661 tctgctttac cgggtaaagc atcaacggtc agtgcagaaa taccatatca gacttttcgt
      721 gactttgctg aaaataaagg tgtgtttacc cccggagtga caggaattga aataaaagat
      781 aataatggaa atgcagttgg tacgcttgat gtgccaatga ttgacttcag cagcgtgtca
      841 cggcggggct cactgaccct gttgtcgcag ggatatggtg tatcagcaaa acacggagac
      901 cttggtgatg taaacaatgc cagttttggc tatgataaaa ataattatac tgtagtaaag
      961 aacaacaaac attcgggcct tgatttttct ctgcatcggt tcagcaaact catcacagag
     1021 gctgcacctg ctgatataaa tatattcgga caacttagtg attcgtcaca gtatactgct
     1081 ttttacagag caggcgctgg cacacagtat attaaagaac gctcaggtaa acagactcat
     1141 atcccgggaa catttttaac tggtggaaca gtaggcactc catggtattc aggaaataat
     1201 ttaatatcaa gctctccagg tgacacctat aataaatcac agggaccatt agccagttat
     1261 gggcagatgg gtgacagcgg gtcgccatta tttgcttatg attctctcag tgaaaaatgg
     1321 tctctggctg gagtcaccct gcataactat ggggtgaacg gttacagaaa taactggttg
     1381 ttattacctg aggattatat cagaaatatt attgtagccg atttcgatcc cattatttca
     1441 tttaataaaa acagtaaaga acatatgtca tggacatatg atgcagccaa aggcgtgggc
     1501 cgtattcagc aggatgacca acagtttgtg atgcatggta acctgaatgg taatctgaat
     1561 gcaggtaaaa atctttattt tacaggcgaa aatggcatta ttgatttaaa agataatgtt
     1621 aatcagggtg ccggatatct tcagtttgct gatgattata cagtaacaac gagcaatgac
     1681 agttcatggt ccggaggagg cattattgtc aattatggta caactgtaaa atggggtata
     1741 aacggggttt tcggagatga ccttcataaa gttggggacg gaacgcttat tattaatgga
     1801 accgggaaaa atgaaggggg actaaagatt ggggctggta ctgtaatcct tgaacaaaaa
     1861 gcgaaaaaca acgattcaac ggctttttct tccattaaca tcagtggggg aaactcgcgc
     1921 gttaaacttt caggagataa tcagattata cctgataatg tttcctgggg attccggggg
     1981 ggatatttag atataaacgg aaaagacact gagttctccc ggttacaggc tgttgattat
     2041 ggggctgcaa ttattaacag cagcatagac aagtcattat tgactttaaa tctgtcacca
     2101 ttgaaaaaag atgaaatagc tgtaagcgtg aaggtgttag atatgaacgc catctttcag
     2161 gggggacacg ggacagccgg agatttgtat aaaacaactt tttacggccc gacacagtat
     2221 tatctgctga aaaaacctaa atttggcagt gtgctgatgg gggcactgaa aaacaccagc
     2281 gaatggcaat ttgcaggaac agacctgaat caggccgttg atatggcaaa gaataataaa
     2341 ctgacatcaa gtgcacaggc ttcatatctt tatcacggta agctcctggg gaatatggat
     2401 attgtcattc cggagcttac aggcaatgat attttaacgc ttgatggctc tgtcagtata
     2461 tcaggagata tgtcaaaaca ggacggtgct cttatcttcc agggacaccc ggttattcat
     2521 gcagggcaaa ctgtttctgc atcgcagagt gactgggaga acagggagtt ctcactcaac
     2581 aatctgaatc ttaataatgc ggacttcagt ctgtcccgta atgcatttat gaacgggaat
     2641 atcagggccg ttaaccagag cactgttatt atcggcggag atacagtctt tactgataaa
     2701 aatgacggaa caggtaatga tgtcatcagt gttgaaggga aatctgctgc cgcaggaaca
     2761 tcctcctata cagggcatat cactctggag caaaaatcag cactggatat ccgcgataat
     2821 tttcgtggcg gggttacgtc tgaagacagt catatcaatg tttcttcatc ttcagtcctg
     2881 ttctcagatg catcgtcatt tataaacagc tccctgaata ttcataaagg aggtgcgctg
     2941 accgctcagg gagggctgtt tacaagtgga agcattgata ttggtgacgc ttcccttctg
     3001 cttaccggta caccagtgaa ttcagatgat gctgcttttt taccgaccat caatatggct
     3061 gatggcggat ttaaactgat gtctgattca tcagtactga aagccagaga ccaggcatct
     3121 gttgttggtg atattatttc tgataaacag gccacaatca gcttcggaac tgaatcaggt
     3181 aaagagggca tattgtctga gaaggcatcc cggggactcg cggtaggatt actgagtggt
     3241 tttaatacgg cataccgcgg tgcaattcat gccccgtcag catctgccac tgtgaacaac
     3301 acctggtggc aattgacagg agactcctca cttcgctcgt taaaaaatac cggaagcatg
     3361 acatatttta caggaagtgc agcgaataaa gcattccata cactgacggt tgatgagctg
     3421 acgacgaatg gcactgcgta tgccatgcgt acggacctga aaaatgcgga taagctggta
     3481 gtaaaccaaa agctgtcagg taaggacaat attctgctgg ttgattttct gaacaaaccc
     3541 accggagaaa aactggatat tgaactggtg agtgcaccgg ggaacagcag taaggatgtt
     3601 tttaaaggaa gtgaacaggc aataggtttc agcaatgtca cgcctgttat tacagctata
     3661 gacgccggag ataaaacaac atggaacctg accgggtaca ggatggcaga aaatcctgcc
     3721 gcaacccaaa gtgcctcagg ccttgcatct gtggggtaca aatcattttt gagtgaggtc
     3781 aacaacctga ataaacgtat gggtgacctg cgtgacatca atggtgaagc tggcgcatgg
     3841 gcacgtatca tgagcggaac cggctctgcc ggtggtggtt tcagtgacaa ccacacacat
     3901 gttcaggtcg gtgtcgacaa aaaacatgag ctggacggac tggatttgtt taccggcttc
     3961 actgtcacac atactgacag cagtgcctct gctgatgctt tcaaaggtaa aacaaaatct
     4021 gtgggggccg gactctatgc ttccgccatg tttgattccg gtgcctatat cgacctgatt
     4081 ggtaagtatg ttcatcatga taatgagtac accgcaacct ttgccggact cggaacccgt
     4141 gattacagta cgcattcatg gtatgccggt gctgaagcag gctaccgctg tcatgtcact
     4201 gaggatacct ggattgagcc acaggcagaa ctggtttacg gtgctgtatc cggtaaacag
     4261 tttgcatgga aggaccaggg gatgcatctg tccatgaagg acagggacta caatccgctg
     4321 attggtcgta ccggtgtgga tgtgggtaaa tccttctcag gtaaggactg gaaagtgaca
     4381 gcccgtgccg gtctgggcta ccagttcgac ctgctggcta acggcgaaac cgtattgcgg
     4441 gacgcatcag gtgaaaaacg tatcaaaggt gaaaaagaca gccgtatgct gatgtccgtt
     4501 ggcctgaatg cagaaatcag ggacaacgtc cgctttggac tggagtttga gaaatccgcc
     4561 tttggtaagt acaacgttga taatgctgtc aacgctaact tccgttactc gttctgatac
     4621 tccgtattca aaagggcctg agggcccttt atgctcagga cctgccgccc gggtatacat
     4681 ttccggagta tcagtcgatt tgtttactta accgccatga ccaaagccgg cagtaatgtc
     4741 ggctttttac gtccatatat tccactcatc cggaactgtg acacagaacc tgaatacagg
     4801 attgatcacc ggtgcccaca aatggcacat tttcagtaag attctgaatg ttacattccc
     4861 ccttcatgtg tgacacagct gtcacagaga acgaaacgga aagcacatga aaaacataaa
     4921 aactcacacc ggactcctta tccacaaaga acaaacacgg cgcgttcgtc ttcatgaaac
     4981 accggccgcc tggtgccaca cacgccggga atgctacagc aaaacgaccg gtcgtcgctg
     5041 cggcagccct gacagtctca gcaggctgat tctgagcagc attcagccca tagaatgaga
     5101 taacccgtat gaatgatgac agacgtctac ggtacccgat gtatggaaca tttgctttct
     5161 ggctaacggc atgcccgaca catggtttta caccgatgac gcctccgtca caaaagagtg
     5221 ggggcgggaa tattgtgaca atgtggaagt gttccctctg tgctatatgc atgacggtcc
     5281 cgttttccgg gggcgctata ttgatgagct cagcgccgca cggaacagaa tgctccatat
     5341 gcaccacata ctgccggaat gaacccggta cagagaaaat tttcatgacg cagaatgtat
     5401 ccgtgaaatt cacgaagcgc ccctgcaggc tcccggcatt ctgcaaaaat acaaacgcac
     5461 aaaatcataa g
//
LOCUS       DAZGTJ010000178         5399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000178 DAZGTJ010000000
VERSION     DAZGTJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.178"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            138..1220
                     /locus_tag="ACHK8E_004951"
     CDS             138..1220
                     /locus_tag="ACHK8E_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFP0347076.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1409..1792)
                     /gene="quuD"
                     /locus_tag="ACHK8E_004952"
     CDS             complement(1409..1792)
                     /gene="quuD"
                     /locus_tag="ACHK8E_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFP0347077.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1878..2018)
                     /locus_tag="ACHK8E_004953"
     CDS             complement(1878..2018)
                     /locus_tag="ACHK8E_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0347078.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2015..2377)
                     /gene="rusA"
                     /locus_tag="ACHK8E_004954"
     CDS             complement(2015..2377)
                     /gene="rusA"
                     /locus_tag="ACHK8E_004954"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP0347079.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2374..2664)
                     /locus_tag="ACHK8E_004955"
     CDS             complement(2374..2664)
                     /locus_tag="ACHK8E_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0347080.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2657..2827)
                     /locus_tag="ACHK8E_004956"
     CDS             complement(2657..2827)
                     /locus_tag="ACHK8E_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0347081.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2827..3282)
                     /gene="ybcN"
                     /locus_tag="ACHK8E_004957"
     CDS             complement(2827..3282)
                     /gene="ybcN"
                     /locus_tag="ACHK8E_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP0347082.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3279..3380)
                     /locus_tag="ACHK8E_004958"
     CDS             complement(3279..3380)
                     /locus_tag="ACHK8E_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347083.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            complement(3497..4294)
                     /locus_tag="ACHK8E_004959"
     CDS             complement(3497..4294)
                     /locus_tag="ACHK8E_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0347084.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            complement(4304..4855)
                     /gene="ybcL"
                     /locus_tag="ACHK8E_004960"
     CDS             complement(4304..4855)
                     /gene="ybcL"
                     /locus_tag="ACHK8E_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP0347085.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
BASE COUNT         1538 a         1228 c         1077 g         1556 t
ORIGIN      
        1 agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta catcttgaaa
       61 taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta ataatagttc
      121 aaaaaaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag
      181 tactgatggc gatgtctgct caggcagctg aaatttataa taaagacagt aacaagctgg
      241 atctatatgg gaaagttaat gccaagcact acttctcctc taatgatgca gatgatggtg
      301 atactactta tgcccgtctt ggcttcaaag gtgaaaccca aatcaacgat caactgactg
      361 gtttcggtca gtgggaatat gaattcaaag gcaaccgcgc tgaatctcaa ggctcctcca
      421 aagacaaaac ccgtcttgca tttgcaggcc tgaaattcgg ggactacggc tcaatcgatt
      481 acggccgtaa ctacggtgta gcatatgaca tcggtgcgtg gactgacgtt ctgccagaat
      541 tcggtggcga tacctggacc caaacagatg tgttcatgac tggtcgcacc actggtgttg
      601 caacttatcg taacaacgac ttctttggtc tggttgatgg tctgaacttt gctgctcagt
      661 atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa tggtgatggt ttcggtttct
      721 ccactactta tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa tctgaccgca
      781 ctaataatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa aatgctgaag
      841 tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctacc acctattctg
      901 aaacccagaa catgactgtt tttggtaata accatattgc caacaaagca caaaacttcg
      961 aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct tacctgcaat
     1021 ctaaaggaaa agacttgggt gcgtggggtg atcaggacct ggttgaatat attgatgtag
     1081 gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgattacaaa atcaacctga
     1141 ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc gttgctgtag
     1201 gtatggttta ccagttctaa tttgattact aaaagatatg ttgcgggagg ctttgcctcc
     1261 ccaacatata agtggctccc tcaagccact tcctttagga gcacaacctt gcttctaact
     1321 atataaacct tctgttatat attacccttt atttttgggg gcgttgcaac gccccatttt
     1381 taataatttt tagtaaacaa ttggcatatt aattagagtt attaacaacg atatccatct
     1441 ctaaccggat atctaatgcc attaacatcc cttcaattat gccctcagcc ttctgtaacc
     1501 ttttcccgat ataaccatca gagcagcaat gcttacctgc cagtgacatg aatgtcatac
     1561 cgactacata ataatctact aataaatcgt gcaaatcgct gttgttcttt ttcagacggg
     1621 ccatgcaccc gcaaataatc atcgcgtcat cgtcacaaca ttgcgggcga gattttactt
     1681 ttgaagtaat taatccctta aaaccggcgg caatggacga ccaggtcaca tcttcatgat
     1741 tattagccgc ccacgctccc caacgctcaa gaaccatctg aatatcacgc atcaacttac
     1801 tccacaaaaa tcagaccaga acgccaatta caagcaaaaa tcaacaaaac agtattagtt
     1861 gattgttatc tctgacttca tactcctgct cctgtcaggg ttttggcgta attcttcagt
     1921 attcggtaat cggtcaaaac agaaccgggg aaacgatata agcgcagatg cccccagcgg
     1981 tggcgaagaa gttctgccat ataaaactca aacatcattc attccccatt tcggtgatgg
     2041 tcagttccag cctgccacct ttggtaacag gcatcttcac aacgcggtaa tcaacgacct
     2101 gagcatcatc cagccagaaa cctgctttgg tgagtgcgtc aaaagcggct ttttgcagat
     2161 tatccaggtc acggcgacgg cgatccggca tgtggcactc aatgcggatt ttcacaggca
     2221 tagccaggcc gatatccagc attgcgtttt taatgattcg ggcgacgtta tcgcggtatg
     2281 cctgcccctc tgcactgacg tgcgtgcgcc cgcgattatg gcggtaatag cgattattgc
     2341 tcggaggcca gggtaatgtg atactgtagg tattcacgcc ttaataaccc cctctttcag
     2401 ccagataacc tgtgttctcg ccataccttc cagcgcgcat tcttttgcat atgcagcatc
     2461 gacaaaatgt gtgcggcggt cgatttcgtc gtggcaggca gaacatgcaa tggtggcaat
     2521 caggtctggc ggtttgatac cggtaccgca caatccagcc agccggatat gtgccagtac
     2581 agacgtttca gaattgccat tacatacgcc agggattctt acctggcatt cccgaccacg
     2641 cgctgctttt ctcaaatcag ccatgattcc tccttgctgc cagtcgcaac cattttttat
     2701 caaccaggct agcggtatat ccgagcagtg ttggtatttc ggatggcttc agctcaggct
     2761 tacgcttacg acgatttgat actctgtaga tgtgtccgtt catgacacga ataagcggtg
     2821 tagccattac gcctcctgct tgtcgcggag cagctggaac tcgcagctct gcggaatagt
     2881 caggtggcag ccaatattca ccgcccaggc ttcaacctta cacaggaaga catacatctc
     2941 tccggtatca agatcggagg tatggcgtaa cgactggata gtggtgatat caccggttac
     3001 gacatcaacc aggtctttgg tttcataacc gagatatgtg tgtttgagag catcttttac
     3061 ccaagctgga gtggcgaacg ttttacccct gctgatgagg tattcactga tttcgctgta
     3121 ccacatgtgg ctgagtgcat tctgggaaag actgcgtttc tcacgccacg gtttaagcac
     3181 catgcgaaag catttgccct cctccagata aggctggatc tgccgaccga tagcggtgaa
     3241 gttgccgcga tgtaatttga tgccgtcttg tgagaggttc acgcttcacc tccgcagagg
     3301 tcaaacgcta gatgcaaaga attgcaggtg catttctgca tctgtgaagg gagaagagag
     3361 tttggattgt atgtgcgcat aaacgtcccc gtttagcgca gaagtcaccg gagttgttca
     3421 ggctccggtg acataattat gccgtgttga tttcccaaaa tcaaaatcga tagaattgct
     3481 ccttcttaaa acacttttac tctctggaag cttttcttat ctctcttggt gttatattaa
     3541 aacgattatg aaatctttca gtaaaacgag aaggacactt ataaccattt tctctggcaa
     3601 tctcgcttat aggttttacc gtcgtttgta tagcagacaa cgcattattt aacctcacat
     3661 cgtccagtat actttggaaa cttaccccct cgcttgctag acggcgatgt aatgtagaaa
     3721 cagaaatgta gagatatcga gcaaccttgt ttgctgtcca ttttgtgccg ggttcggata
     3781 gcagcaggtt ataacaacga cttatcaatg attgtttact atatgataaa agtaaatgat
     3841 taacatgatt cactcctaac gaaagtagaa cgcccattgc taagtgctcc tgaattttag
     3901 ttgagaagcc tcgggaaaca gatgttttta gttgctccca acaatatatt aactcaggat
     3961 tctgaggtaa aaagaaactt gttttgttac gtatttgatc agttaccgta taaagttttt
     4021 ggaaactctc aattaaatca atgggtaagt aaagcatttc tgcaagataa agccctgctt
     4081 caggataatt ctcaatataa aattcataac cacaaggaaa taatattatt tgattattat
     4141 caacagttaa agtatgcgtc tcccaattga taactttctt tccctgacgg atacgacaca
     4201 aagctggcat aagaggctta accctatgaa tctcatgatg tttatgcatc cgtatttctt
     4261 cgatctttaa gttagtctta cctcttgcca gcatactctc accctacttt atctcataaa
     4321 ctggtgttat ctcagcggtt gcgattttat tagcattaag catataacca actaacgctc
     4381 cgctggagtt agaatctaca ggaatctttt cagtttttag agcccatact ttaaactggt
     4441 aatgatgtgg tttatctcct ttaggaggac atgcgccacc aaacccagca tagccaaaat
     4501 catttcggcc ttgaacagca ccagtcggca gttttgttcc atcacgtctc cctgcatcaa
     4561 cgggcaaata tgttactgtt gctggaatat taacaacagt ccaatgccac caaccactgc
     4621 ctgtaggtgc atctggatca tatacagtta cggcaaagct tttggtacct tcaggaacac
     4681 cagaccaggt taatgagggc gatgtattac caccttcaca cccaaatcca gaaaagacat
     4741 gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga aatgctgcag
     4801 cctgcgcaga aaatgttatg aatgccaata cagttgaaac gataagtgtt ttcataaaaa
     4861 cctctttgtt atgacctatc gttattttat ttgatattcc tttatctcat tatgcataaa
     4921 ggcgcaatgt tcatgcaaaa gcaatcacaa ttgtaccccc aacccaatta tttgccacaa
     4981 tatacacaaa gcacattgat actatctaaa aactctgctt tattattagt aatacctacg
     5041 aaagtcggtg ttatttttta acctaccatt caaaatacgt gacatacacc attttgctca
     5101 taataatttg tcacgtattt tcagtatttg aatctgcgac caagagttct cacctaacaa
     5161 atgattaaga ttgtatagct catttactac cccaatacag ccgtacaaaa ctcgcttgtg
     5221 ggagcaaaca aagtaattac ccattaagtt tcgtcaaaga taattaattc tgtcttgcac
     5281 tttatcacca tagcataact taaaatccga gatcattatt tagaaataaa tctcaccatc
     5341 aaccatatat ttgagagcac ttatcgcctg ctgggcggat attactttca ttaaaggat
//
LOCUS       DAZGTJ010000179         5355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000179 DAZGTJ010000000
VERSION     DAZGTJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.179"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..207
                     /locus_tag="ACHK8E_004961"
     CDS             <1..207
                     /locus_tag="ACHK8E_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0347086.1"
                     /translation="ATGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAL
                     YIDGRREMMQWYADYIDSLSEQVS"
     gene            536..1117
                     /locus_tag="ACHK8E_004962"
                     /pseudo
     CDS             536..1117
                     /locus_tag="ACHK8E_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            1204..1482
                     /locus_tag="ACHK8E_004963"
                     /pseudo
     CDS             1204..1482
                     /locus_tag="ACHK8E_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1750..2754)
                     /locus_tag="ACHK8E_004964"
     CDS             complement(1750..2754)
                     /locus_tag="ACHK8E_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0347087.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTTNQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            2803..3758
                     /locus_tag="ACHK8E_004965"
                     /pseudo
     CDS             2803..3758
                     /locus_tag="ACHK8E_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3798..4277
                     /locus_tag="ACHK8E_004966"
                     /pseudo
     CDS             3798..4277
                     /locus_tag="ACHK8E_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            4420..4649
                     /locus_tag="ACHK8E_004967"
                     /pseudo
     CDS             4420..4649
                     /locus_tag="ACHK8E_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4636..>5355)
                     /locus_tag="ACHK8E_004968"
     CDS             complement(4636..>5355)
                     /locus_tag="ACHK8E_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0347088.1"
                     /translation="ALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSF
                     ASRKTDDMLLLQETERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLEQYPRKIA
                     CYLASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYSV
                     PLIITASDITSHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFNG
                     ERNVPADSLKPQPLILPVRGK"
BASE COUNT         1463 a         1228 c         1278 g         1386 t
ORIGIN      
        1 gcaaccggtc atggttttcg tcacaccatg agtaccattc tgcacgaaca ggggtttaat
       61 tctgcctgga ttgaaatgca gttagctcat gtggataaaa attccatcag gggtacctat
      121 aatcatgccc tgtatattga tggtcgccgt gaaatgatgc agtggtatgc tgactacatt
      181 gattcgcttt ctgagcaggt aagttaagct gagaacacgt tattctgatt ttcactcact
      241 tcacgattat tcctccggcc tgcaggttcg gaggattctt atttttatgt tttattttca
      301 gataaagttt ttgacaaaac gtttattctg catcagcaaa attatcacaa aacaacgcaa
      361 tcacagacaa attaacattt ttcagacggg tatattgagt gcccacaatg taagacaggc
      421 aggcatccgg tgacatggta aaatattaat ccagggcaaa aacaaggctt gatacacaac
      481 atcatgactt ttatgctctc agactatgcc accacagatt ttacaggctc agaaaatgaa
      541 tgacagatta cgctttgaag tgaacgataa ccagggctgt ttcgtgttcc cggacgcctg
      601 gtttggctcg ttgctggatg agtttgacga actaattgat gcatacgaca gagatgaaat
      661 cagcgaaaca agttatatca acaaactcag acgccttgcc cgacaggagc ctgactttat
      721 tgatgtacac gcttacctgg catgcgcatt tctggagcag aatgccccgc gtaaggcact
      781 gaacgctgct cttaaaggac tggcagcagg taatcgtctc atcccggaaa gtttcagtgg
      841 tgaaatcatc tggatgcatc ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat
      901 tctcgcaaat gtgcacttac agcgtcatca ggatgcagct ctgctcactg ataaaatact
      961 ggcatacaat cctgaagaca atcagggagc acgctggcta ctgggaccgg aactgttacg
     1021 tactggcgac catgaacagg cacgccatgt actgaaagcg catgcagatg aattttcacc
     1081 ttactggtat gaactcggcc ttctgcattt tctgaaccgg ccccttgaaa cgccagacag
     1141 tggatgtatc ttaaaataag agataggctt ggatatatgt ctaacactag ttctaatttt
     1201 gagatgactg gaattttgct ggggcaggag cttcgtaaac gtaaaactcc tcaggaaaaa
     1261 atcgccatta ttcagcagac aatggaaccc ggtatgacgg tgtcccatgt ggcgcgtttg
     1321 catggtattc agcccagcct gttgtttaag tggaaaaaac aatatctgga aggaagtctc
     1381 actgctgttg ctgccggaga ggatgtcgtt cctgcctcag aactggctgc cgctataaaa
     1441 caaataaaag agttacagcg cttgctgggt aagaaatcta tggtccccac catttttgca
     1501 acactgattt tgattgatgt tggcttgcct aaatctatcc ggcgtcatta tgggcatgga
     1561 tgcccgcgcc tcgatgagtt ccgcacctga tgagcccaaa aaaccatccg gcttcagtga
     1621 gccattttct tgctgaggct tttcatcagg ccgatatgcc gttcatgtca tcaataatca
     1681 gtcttcgcaa aaccagattg ggaactactt taattcatga ttaacctgcc agccagactg
     1741 gcgccatcgt tacctttcga acacagtctg ctgtgttgtg attgcccacg ctatccgggc
     1801 taatttgttg gccagtgcgc aacatactac gctggagtgt tttcttgcca acaacgccct
     1861 gacccaatcc gccaaccttc ctgactgatt ctcgattctc tgaataaagg atcttgcgca
     1921 ctgaacgagt aaccgtcgca gatttttatc tccacgttta cttatcccta aaagtgtatt
     1981 tttaccacct gtgctgtact gccggggtac cagaccggtt gaagcagcaa agtcccgact
     2041 gcagctatat tgtttaccgt ctcccagctg cgatgacagt acgctggcgg ttatcggacc
     2101 aataccgggt attgtctgaa gacgttgtcc tacctcatca cgactcagtt cctgctccag
     2161 tgcgatttca agctcggtga tctgtccgac aagataaata taatgggcat gtaagcgcat
     2221 cagtaactgc gccagatagg gaggaagttc gttctctgca agtactgttg aaagtcgttt
     2281 taccactgcg actcctgtcg gcatactgat gccaaattcc agcaaaaaag catgcatctg
     2341 attggtcgtt ttgactttat cactgaccaa tgattccctg acccggtgaa gcgcccgcat
     2401 ggcctgctga gcttctgttc gtggttgcac aaatcgcatg gagggacgag atgccgcttc
     2461 gcatatggct tctgcatcga caaaatcgtt cttgttgctc ttaacaaaag ggcgaacgaa
     2521 ctgcggagaa atcaattttg cttcatgacc taaatcagca atccggcgag ccataaaatg
     2581 agctccggcg caggcttcca tcacaacggt agacgaggca cagcctgcca gaaactcaat
     2641 aagtttggta cgggtaaact ttttacgcag caataccttg cctgacttat cttggcagtg
     2701 aatgtgaaag gaatgcttgc caagatctat accaataagc gtaatgtttt gcatgatggt
     2761 cttctccatg aaagaaaaac actctactag ggtacagctc acagggtggt ggggaccatc
     2821 tcattaacca tggaagttga gatcctcaaa gaagcggtgg agtatggtca gtcgcgaaaa
     2881 tggatagcgc atgcgccctt gttgccaacg gacaaggaat aaccctggta agccgtattc
     2941 tgggcgtgtc gcgtgcgcaa ctggcacaac ggatgaaaac atctggtaat aagccgaata
     3001 aacgtcgtcg cagatgtgat gaggctgcgg atgcggaagt gttagcacgt atccttgata
     3061 tcatcagcga tatgccgaca tatggctatc gtcgtgtctg ggccattctc agacgacagt
     3121 cccgtaacaa aggagtgccg ttcgtgaatg caaagcgcgt ttatcggata atgagcgaaa
     3181 ataacctgtt gttacatgac aaaccttcac ggccgcagcg ggaacataaa ggccgaatat
     3241 cggtgaagga aagcgaccag cgctggtgtt ccgatggctt tgagttcggt tgtgatgatg
     3301 gcgcaaaact gcgtgtcacg ttcgcactgg actgttgcga tcgtgaagcc atagactggg
     3361 ccgcaagcac gggaggttat gacaaagcaa cggtgcagga tgtcatgtta ggagcaatag
     3421 aaaaacgctt tggcgataaa ggagcggaac ggccaatcca gtggctgact gataacggtt
     3481 cggcttatac agctcatgaa acacggcagt tcgccagaga gttgaatctg gagccctgca
     3541 tgacggcagt cagtagccct cagagcaatg gaatggcaga aaggttcgtg aaaacgatga
     3601 aagaagacta catcgcgttc atgccgaaac cgaatgtaag aacggcattg cataaccttg
     3661 cagtggcgat cgagcattac aatgaaaccc atccgcatag tgcgttaggt tatctctcac
     3721 caagggaata tcgacgtcag cgggtaacgt caacttaaga tacaacgact gtctgaatat
     3781 cgtgggtcaa ggacagcctt ctgcattttc ttaatgggga actggtaaaa gctgcaactg
     3841 catttcgtcg cggctttgcg gcaaatactt acatcgcaga aatactgtgc ggaaatctgc
     3901 atccatttcc acttgctgta tggcataact tttcaggcgg tccggatact gctgaagatt
     3961 attatgccac ataccacccg ttatgggggc aatatccgga agcattactg tttgtaaact
     4021 ggctctataa tcattcctct gtgctgcatg aacgggcaga aatcattaaa tgcgccgaaa
     4081 tgctgatgca ggaagatgat ttcgaaatat gcgaaagtat tttaagacag caggagaagt
     4141 tgcgtgaaag aattgatgag acgctttctg agaaaattgt acagaaatgc agaaatatga
     4201 atggtgaata tgtctggccc tggatattgc cgttttcagc ggcaggcatg aaacatactg
     4261 gcatacagta tcagtagata ttgcattagt gtatcctgca cacaagtaat aatttatcca
     4321 ccaataataa cactgttaat gtccccttcc cctggttgtc agccaggggt tatcttctga
     4381 atatttcttt tgaaaaggat aacacagtaa attattttta tgaattatcc catggactca
     4441 ttaacaccct ttcataatgc tttattgtca aacacgttat ggctgacatc aaaaaaaccg
     4501 gatttcctct gccagcgggt aatcacctcc ccggtgtttt cggttggtct ggttactcca
     4561 gtctggtaat tagcaagata atcgatataa acagtggaaa actcatcgta cataatctgg
     4621 tgatgaacat tacgcttatt ttcccctgac cggaagaatc agaggctgcg gtttcagact
     4681 gtctgccggt acattcctct ccccgttaaa aaccataacg ggttcattat cttcgtctgt
     4741 cagcggattg aatggcggta tattttcagt acgaatgccg gtcagccact gaaaaatacc
     4801 tgcgaaatga cgggcactga ttttcctgct gacggactga tgagacgtga tgtcactggc
     4861 ggtaataatc aggggaacgc tgtagcctcc ctgcacatga ccatcatgat gaacaggatt
     4921 ggcactatcg ctgaccgaca gcccatggtc agaaaagtaa agcatggcga aatgacggga
     4981 atgccggcga aggataccat caagctgacc gagaaagtta tccagtttac tgatgctggc
     5041 gaggtaacag gcaatctttc ggggatactg ctccaggtaa tgattcggcc aggagttaag
     5101 tcggtcacac ggattcggat gagagccaat catgtgcagg aaaatcacct tcggagagga
     5161 tttatccgcc agcgcacgtt ctgtttcctg taacaacaac atgtcatccg ttttacggga
     5221 agcaaagctg cctttcttga ggaaaacggt atgctccgca tcagaagcaa taacagagat
     5281 gcgtgtatca tgctccccca gttttccctg attggatatc caccatgtgc tgtatcctgc
     5341 ttttgctgcc agcgc
//
LOCUS       DAZGTJ010000180         5347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000180 DAZGTJ010000000
VERSION     DAZGTJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.180"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            88..807
                     /gene="fabG"
                     /locus_tag="ACHK8E_004969"
     CDS             88..807
                     /gene="fabG"
                     /locus_tag="ACHK8E_004969"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0347089.1"
                     /translation="MRNKVCIVTGAAQGIGRKIVEDFSKSEVKRVYACDIKHTDKSYP
                     ENVTPVILDVCDRDSINTVISKIYSDEGRIDVLVNNAGVTRDNLINNMTEQDWDIVID
                     INLKGVFNITQAVAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKGWA
                     KEFSRKGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPEDISNGVLFL
                     ASEKSSFITGQTLKIDGGLVI"
     gene            817..2025
                     /locus_tag="ACHK8E_004970"
     CDS             817..2025
                     /locus_tag="ACHK8E_004970"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008913448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0347090.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFAFESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILASEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIALGHPLGASGSRIVVSLLYQMIKNNKKRGLASLCIGGGM
                     GTAVIIELCS"
     gene            2095..3432
                     /locus_tag="ACHK8E_004971"
     CDS             2095..3432
                     /locus_tag="ACHK8E_004971"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFP0347091.1"
                     /translation="MIGLIISLILLIYLIYRGFPVLIVSPLMAMLAVFMGSDIPVFYS
                     WTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASGAALTIAHFISDKMGEKRAIIAVII
                     ASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQRLLPSAICAGCLPAYVAPGSAQFI
                     NAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYMSAKIKNAKIKGEGYGTEVSNDCNL
                     ETGKVTLAFIPILIVIIGNAFLTYFFQIDEVVDYYRDFGGVQGTWAILISIAIAIVVT
                     ICIYWNTFKSDIKKTLTLGATESLLPIFNTSTQVGYGGVIKVLPVFTLIQTTIFSIPG
                     VALFPAVIGTAILSGIVGSTSGGVGLVMVTFGQDLLELSQAQNISPGLMHRIIVFSAS
                     TLDTLPHSGFIITLLGVCGLSHKQSYKELFIVTCVFPAFAVLSMLVYAFLFLI"
     gene            3445..5010
                     /locus_tag="ACHK8E_004972"
     CDS             3445..5010
                     /locus_tag="ACHK8E_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0347092.1"
                     /translation="MSIYSRKQCTLEDAVNMIHDNDCVILGGFIGAVVPECIEKGIED
                     RFLKTGKPRNLKIIFAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGK
                     ITGYNLPQGVICHLFRDSAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEI
                     NGVENLFYKKIDANIAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQV
                     KQIVRDGTLDPLQVRIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETR
                     PLDIKKIIARRAAMELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTP
                     AGGLSFGASSFPQAIITQDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAGC
                     GGFINITQNAKKVYFCGSFTAGKNKIYVSNGKLHIKEDGSVKKFIKKVQQITFSSDTA
                     RKNNKEVFYITERAVFRLSQKSIELIEIAPGVDLQKNIIECMEFVPEISPSLKIMDER
                     IFLEQPMGLELKD"
     gene            5013..>5347
                     /locus_tag="ACHK8E_004973"
     CDS             5013..>5347
                     /locus_tag="ACHK8E_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFP0347093.1"
                     /translation="MVYTIDKLHLGQKASFEKTITEADVLIFSGISGDINSVHINEEA
                     AKKSIFKKRVAHGVLVSSLTSAVLGMKLPGEGTIYLRQENNFRKPVFIGDTVKATCEV
                     VDINKEKNIV"
BASE COUNT         1699 a          774 c         1175 g         1699 t
ORIGIN      
        1 tttactttat gaacgagaat ggttaaaaga tgttttgttg caaaacgaaa ggcatcctgt
       61 tctatattca aaaatgaggg aataaaaatg cgtaataaag tatgtatagt tactggagca
      121 gcacaaggta taggaagaaa aattgtggag gatttttcta aatcagaggt aaaaagagta
      181 tatgcatgtg atataaaaca tactgataaa tcctatccag agaatgtcac tccggtaatt
      241 cttgatgttt gcgatcggga ttcgattaat acagtaatta gtaagatata ttctgatgaa
      301 ggtagaatag atgttttagt taataacgca ggagttacaa gagataatct gatcaataat
      361 atgacagagc aagactggga tattgtaatt gatataaacc ttaaaggtgt ttttaatata
      421 actcaggcag ttgcacctat tatgatggaa caaaatcatg gggcaatcat aacaatgtct
      481 tctgttgtag gaactgatgg taatataggt cagagtaatt atgccgcaac taaaggtggt
      541 gttattgcaa tgtctaaagg ttgggctaaa gaattttccc gaaaaggtgc taaagtacgc
      601 gtgaattgtg tagctccagg attcgttatt accccgatga cagaaaatct gccagagaaa
      661 gtcattaacc atatgatagc taaaacccct ttagggcgaa tggcaacacc tgaggatatt
      721 tcgaatggtg ttcttttttt agctagtgag aaatcatctt ttattactgg tcaaacatta
      781 aaaattgatg gtggattagt tatttaagga ggaggaatgg aaaaagtata cattacaggg
      841 gcaaaaagaa cgcctatagg atgttttggc ggttcgttat caacagtgtc agcttcagat
      901 cttggagcaa ttgcgatatc tgcagcaatt aaacagagca gaataaacgt tgaatgtatt
      961 gatgaagtta ttgtcgggaa tgttttgtct gctggacaag gaatggggcc aggtagacaa
     1021 gctgctgtta aagccggtat accatggaaa actccggctc tgactttgaa tatgatttgt
     1081 ggtagtggta tgaaaactat tatcgatgct gcaatgaaaa ttcgtagtgg agatatggat
     1141 gttgttgtta cggcaggcat agagaatatg agtcaggcac cttacttgat tgatcatcat
     1201 gcaagaagtg gtgtacgaat gggtaatcag gtaatgagtg actctcttat tacagatggt
     1261 cttactgatg cttttaatca ctatcatatg gggattacag cagaaaatat agcccgacag
     1321 ttcaatatta caagatatga acaggataca tttgcgtttg aaagtcagaa gagagcctta
     1381 aaggccattg acaatggaga atttgaggat gaaattactc cggtacaagt acatacccga
     1441 aaaggaaata ttgaagttac tacagatgag tatcctcgta gagactcctc attagagaaa
     1501 ctttctgtgt tacgtccagc atttgaaaaa aatggtaccg tgactgcagg aaattcatct
     1561 ggaataaacg atggtgcagc agctcttatt cttgcttcag agaaagcaat tcagacattg
     1621 gaattacaac ctttggctga aattcgctca tttggacaag gaggcgttga tgcatcagtg
     1681 atggggctgg ggcctgttcc agcaatagat aatgcattaa aaaaagcaaa actggatatt
     1741 aaagatattg atctttttga aattaacgag gcgtttgcgg ctcaggcaat aggggtctta
     1801 aaaagtattt ctgaaaatca ctcagttagt attgattggc taaatcaaag aacaaatgtt
     1861 aatggaggag cgatagctct aggccatcct ttgggagcct ctggctcaag aattgttgtt
     1921 tctttattat atcaaatgat aaaaaataat aaaaaaagag gtctcgcttc cctttgtatt
     1981 ggtggaggaa tggggacagc tgttattatc gaattgtgca gttaactttg aaataagtat
     2041 ctcttgcttg tcaggagata cttaaataat aatattaata ttgagagtta taatatgata
     2101 ggcttgataa tttcacttat tttactaata tatctcatat atcgtggttt tccagttctg
     2161 atagtatccc ctttgatggc aatgctggct gtctttatgg gaagtgatat tcctgtcttt
     2221 tattcatgga ccggtccatt tatggactcg gttgtgagtt ttatatccgc atacttccct
     2281 atatttcttg ttggtgctgt ttttggaaca ttaatgaatg caagtggagc agcattaact
     2341 attgcccatt ttatatcaga taaaatgggt gagaagaggg ctattattgc agttattatt
     2401 gcatcatttt tattaactta tggaggaata tcagtttttg ttgtggtttt tgcagtttta
     2461 cctattgcga gaactatatt tataaaaagt aattaccctc aaagattatt acccagtgct
     2521 atctgtgctg gttgcttacc agcatatgta gctcctggtt ctgcccagtt tataaatgca
     2581 ataccaattc ctgtgttcaa tacaaccata tattcaggtt tagtaattgg tatcgttggc
     2641 actgctgtat ggttattgtt cgctgtatgg tatatgtctg caaaaattaa aaatgctaag
     2701 ataaaaggag aaggatatgg tacagaagta agtaatgact gtaatcttga gactggaaaa
     2761 gttactttgg catttatacc tatactgata gttattatag gaaatgcatt tttaacatat
     2821 tttttccaga ttgacgaagt ggttgattat tacagagatt ttggaggtgt tcagggaaca
     2881 tgggcaattt taatttccat agcaattgct attgtcgtca ctatttgtat ttactggaat
     2941 acttttaaaa gtgatataaa gaaaacactt acattaggtg ctacagagtc tcttttacca
     3001 atatttaata catctaccca agttggttat ggtggggtaa ttaaagtcct tcctgtgttt
     3061 acccttattc aaacaacaat tttttctatt cctggagtag cactatttcc tgcagttata
     3121 ggtacagcaa ttctttcggg aatagtgggc tcaacatcag gaggggtggg attagttatg
     3181 gtaacgtttg gtcaagactt attagaacta tctcaagctc agaatatttc gccaggttta
     3241 atgcatcgta ttattgtttt tagtgcatca acgttggata cgcttcctca ttctggcttt
     3301 attatcacat tgctaggagt ttgtggctta tctcataagc aaagttataa agagcttttt
     3361 attgttactt gtgtgtttcc cgcatttgcg gttctctcca tgttggtata tgcattctta
     3421 tttttaattt aaaagaggtt taatatgtct atttattcaa gaaaacaatg tacactagaa
     3481 gatgccgtaa atatgattca tgataatgat tgtgttattc ttggcggatt tattggtgca
     3541 gttgttcctg agtgtataga aaaaggcata gaagatcgtt ttctgaaaac gggaaaacca
     3601 agaaatctca agattatttt tgctgctggt caaggggatg caggaacaaa agctgtcaat
     3661 catttatctc atgaggggct tgtcactagt gccattggcg ggcattgggg actaatacct
     3721 aaattgcaga agttagctaa tgagggaaaa attacgggtt ataatcttcc tcaaggggtt
     3781 atttgccatt tattccgaga tagtgctgct gggaagttag ggacattaac tcatgttggt
     3841 ttaggtacat ttgttgatcc ccgcgtagag ggcgggaaaa ttaatgataa aacacaagag
     3901 gatattgtgt ccgtcacaga aattaatggt gtggaaaacc tattttataa gaagattgat
     3961 gctaacatag caatcctcag aggtactaca tctgatcaga gtgggaatat aacaatggag
     4021 gatgaatgtt tgattcttga aaacctggct gctgcccagt tagtccataa taatggaggg
     4081 aaagttattg ttcaggtaaa acaaatagta cgtgacggta cattagatcc tttacaagtc
     4141 agaattccag gtatttatgt tgatgctgtt gttgtttctg aagatgaata tcatatgcaa
     4201 acttttgcgg agtatatgaa cttcagctat gtcgggaaag gtgtaaaaga ggtaaaagac
     4261 aaagaaacac gccctcttga tataaaaaaa attatagctc gacgagccgc aatggaactg
     4321 aaaaaaaatg cagttgtaaa ttatggcata ggggcacctg aagttatagc tgaagtaaca
     4381 gatgaagagg ggattacctc cgaacttatc gcaactgttg aaccaggtgc aattggagga
     4441 acgcctgctg gaggtctgtc atttggagct tcttcgttcc ctcaggcgat aattactcag
     4501 gatcagatgt ttgatttcta tgacggtggt gggcttgatc aagcgtttct tggattagct
     4561 gaaacagatg aaaaaggaga tttaaatgtt tctaaatttg gaacaaaaat tgcagggtgc
     4621 ggaggattta ttaacataac tcaaaatgct aaaaaagtat atttctgcgg ttctttcact
     4681 gctggaaaaa ataagattta tgtcagcaat gggaaattgc atataaaaga ggatgggagt
     4741 gtaaaaaaat tcattaagaa ggttcaacaa atcactttct cgtcagatac tgcaaggaaa
     4801 aataataaag aagtgtttta tattacagag cgtgctgttt ttagactttc tcaaaaatcc
     4861 attgagttga ttgaaatagc gcctggagtc gatttgcaaa aaaatattat tgaatgcatg
     4921 gagtttgttc cagaaatttc tccgtctttg aaaattatgg atgagcgtat ttttttggaa
     4981 cagccgatgg gattagaact aaaggattaa tcatggtata cactattgat aaactacact
     5041 taggtcagaa ggcgagcttt gaaaaaacaa taacagaggc agatgtttta attttttctg
     5101 gtatttctgg cgatattaat tctgttcata ttaatgagga agctgcaaag aagagtattt
     5161 tcaagaaacg agtagctcat ggtgttcttg tatcatctct aacatcagca gtattaggta
     5221 tgaaactccc aggtgaaggc acaatatatc ttcgtcagga aaacaatttt cgcaaacctg
     5281 tttttattgg tgacactgtt aaagcaactt gcgaggtagt tgatattaat aaagaaaaaa
     5341 atattgt
//
LOCUS       DAZGTJ010000181         5300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000181 DAZGTJ010000000
VERSION     DAZGTJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.181"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(55..2541)
                     /locus_tag="ACHK8E_004974"
     CDS             complement(55..2541)
                     /locus_tag="ACHK8E_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0347094.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTK
                     KEGVTDDEGCTETFFSDKEEEIEIEILLGV"
     gene            complement(2589..3206)
                     /locus_tag="ACHK8E_004975"
     CDS             complement(2589..3206)
                     /locus_tag="ACHK8E_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347095.1"
                     /translation="MKTNWIEHMKNLLVKKPEVKDFFSKDKFPLNLVDGSYPIFISLK
                     DRGIYAAHSIFICSDDELHAIKEHLSNIFNVNKDTIGNEGVIYDSGIVHHPLFRDAIE
                     IQKTYSLIYTFTKSFKGNLIDLVTNSSFFVETIKSAGIKEPVPWDVFPELEPDTFGSL
                     QGELEFWWESIWSPFWHSLSPDERNDFLVKNNATPQWREFIEFHC"
     gene            complement(3196..4086)
                     /locus_tag="ACHK8E_004976"
     CDS             complement(3196..4086)
                     /locus_tag="ACHK8E_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFP0347096.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
     gene            complement(4080..>5300)
                     /locus_tag="ACHK8E_004977"
     CDS             complement(4080..>5300)
                     /locus_tag="ACHK8E_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0347097.1"
                     /translation="RQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDS
                     AHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQ
                     QRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAPAMAILNGAQSQMKSLST
                     DAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAIASGEHLQLAAAKNVIAN
                     AGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAQKSIEI
                     TSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNVKAGYYGRKPKAKLTPEL
                     MAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKGFTDSAGCCERIFTEEAD
                     NLNVLLGIKALEKW"
BASE COUNT         1165 a         1434 c         1203 g         1498 t
ORIGIN      
        1 ttaatattac atttttgctc atttttttct ctttaataat taagggtggt atttttaaac
       61 accaagtaaa atctcaattt caatttcttc ttctttatca ctaaaaaatg tttcggtgca
      121 tccctcatca tcagtgacac cttccttttt tgttccatca ataaaattgg ctatatactt
      181 aacattacga tatgggatat tttcgtcatc aaaaaattta aaacagcgag aatagtctcc
      241 accctcaatc accggaaacg ccatcagctc cggcgtcagc ttcgccttcg gcttacgccc
      301 ataatatccc gccttaacgt tatattctcc cggcgttccc gcttctatcc cacaggcatc
      361 cagccggatg tagctgccac cgccgttaag ggtgatcttt ttcttcgccg tgattttaat
      421 ttcatcttcg gtactggtaa tgatgatttc cttcttcgcc aacagttcca tcaagtcgtt
      481 ctgcgcctgc accgagattg cgcccttatt ggcaaacagc ttgatgcccg ctttacgcac
      541 aaacacgctc agcgcctgtc ccacgccgat aaacatattt ttcaccacgc cgacatcggc
      601 gttgttgcct gcgttggcta tcacattctt tgccgccgcc agttgcaggt gctcgccgct
      661 ggcgatcgcc acgcctttgg gtgcgcttag caggatcacc gcctgcgtca gatcttttac
      721 actctgctgc aacagcgcta tctgtgatga cagatctgcc ggatcggcat tcgccgtctg
      781 cgcatcagtg gacagcgact tcatctgtga ctgtgcgcca tccagtattg ccattgccgg
      841 agccatttcc cgcacctgac cctgagcctt gtcctgcgca tcggcgctga taaagatgcc
      901 tttcttcgcc cgcactgcgc cccacatatc ggtacgcagt tcaaagcctt cgccgcgctg
      961 ctgtttcccg gcatccacca gatggccgag gtttagctgg ctcttgccgc cgtactcggt
     1021 actgaccttg atatgctctt tgccgcgctc atcgtcgagg cggattttgt tgtttgctgg
     1081 ggtgcgcagc acgttacgct tgtagttctg gatggtcaca tggtcggtgt gcgccgagtc
     1141 gtgctttacc ccggcgatat acggtcggtc cgggttgcct tcctcaaacg cgatgctgac
     1201 ctccgttccc gccagcagcg gcagatgcag gccgtaggtg tctcccgcat acgggcgcga
     1261 ctggcgtacc cacaggctct cataacccgg tttccacgta tcgcggtcaa aatcgaggtt
     1321 gacccggtag cgcccgtctt tgtcgatatg ggcatagata tcgttcttca ccgtgctggt
     1381 gacccgcgcc gggagcgttc cggccatgac cgggcggggg ataaacgccg ggcggtagcc
     1441 gtagcgttct gagtacggga tagcggtaaa cgtcagttca tagctgcggt cacgcgccgc
     1501 gctggccgtc acgccggtaa tcagtacgcc cttgcggaac accgctggcg cgtcgtcgcc
     1561 ctgaaccctg atttccagcc ccggcatcag cagtgaactg gtgctctggc ctttgagaat
     1621 ggcctgttca ttcaggtagc gttcgtggcg gatacgggca taaaacgcgc cgctttccgc
     1681 tgcctcttta tcgccttttt gcaggaagtt gtcggcgtag tgataggctt cgccgtaggt
     1741 ggtgttatcg cccccggtgg cgtcatgctg ctctgtcatc atctccgctg tggcggtgcg
     1801 gtagttgtag tcccgcgtgg tgacgctttt ttccaccacg ctgtacgcgg tgttcagccc
     1861 ccatacggct tccgtttcac catcaaacag ccctgaagga tgacgaagcg gcagcgtcag
     1921 cccgcgctca tagccgctct ggtcgtcata aaactcgaca acctcaattt tcagccgggc
     1981 gtcggtggcg aagcggaacc agatgcccac ttccgacagc aggcgtgaaa taaacgtcag
     2041 gtcgtcctcg ccatactgca tcacctgttc gcgggccggg tattcgcttt tgaggttaaa
     2101 cacgaagtcc tgaccgcgca tctggtgccg ctcgcgcagg attttttcga cgatttgcgg
     2161 caccgtcaga ttctggtaga tggcattctg gcggctgcgg gccagcagcg ccatacgcgg
     2221 ctccagccgt acttcgtaac gggcttcgtc ctgtgacgat gacagatgtt taaagccggt
     2281 aatgacgcca tacagggtgc gcaggggggc ctgcaccggc attccctgta ccggcggtgc
     2341 cgtcaggctg aatgcgccgt cctgcatcag caccaactcc ggcgtgatgg ctttatcggt
     2401 actggtaaac tgaatgtcgt agcggaacgg ggtactcagt tgttcgcggc cttcgaaggt
     2461 cagcacatcc accggacttt gcaggccgcg gattttgagt ttgtggtgtg aatggtcaaa
     2521 gtttagggca ggaatactca tcattatttc cctgtataga tagaaatatt ttcgtcatta
     2581 aaaataaatc aacaatgaaa ctcaatgaac tctcgccatt gtggtgtcgc attatttttc
     2641 actaagaaat cgtttctttc atcaggtgat agactgtgcc agaacggaga ccagatactc
     2701 tcccaccaga actcaagttc tccttgtaag ctaccgaaag tatcgggttc caactcagga
     2761 aaaacatccc atggtacagg ttcttttatt ccagcagatt taatagtttc aacaaaaaaa
     2821 gaagagttcg tcactaaatc aatcaaattc cctttgaatg atttagtgaa tgtgtaaatt
     2881 aaagagtatg ttttttgtat ctcaatagca tctctaaaga gggggtggtg tacaatgcca
     2941 ctatcgtaaa tgaccccttc atttccgata gtatctttat ttacattaaa tatattagat
     3001 aaatgttcct ttattgcatg aagctcatca tccgagcaaa taaatattga gtgcgcagcg
     3061 tatatccctc gatctttcag tgaaataaat attggataag acccatcaac taaattgagt
     3121 gggaatttat cttttgaaaa aaaatcttta acttcaggtt ttttaacgag caaattcttc
     3181 atgtgctcta tccaattagt tttcatctat ataatccgct acccaatctg caccaaaata
     3241 accagctgca ccaaaaacaa tcccgccaat aatcccggta gccactgctc ctggcccagt
     3301 agtaacaccc agggacgcgc ctatcactgt tccaatttta gcaccagcta ctgctcctcc
     3361 ccatcctcca atttgcctta ttgcttctgc acctataggt tttatgcttt ttgtttcaaa
     3421 cgatttttca gttgcctgtt ctaaatcata tgcagtgaaa aaaatactaa aaacttgtac
     3481 gacacgcccg gccttaacaa tgttttgagt cgtttttaga gactctggtg taaaaattgt
     3541 ttgtgctggt acttttctac cttcgatcaa gacctcttta tcaatatcat caacatagcc
     3601 tgaaattttt tcaacacgct tagccaaatg agggtattgt ttcttatact cctcaagagc
     3661 agtaataatc tctgcggtcg agattaactt caacccggca ttttttgctt tgtgaacatc
     3721 aataaatatg gttttcccct caaatctcgg agagccatca ggaaatatag agcttgtaga
     3781 aatcaacgag gatttgttgt tccccataac atgttctgct acagtagtgg gtgctgaata
     3841 atcttttggc atcaacccat agcgttcagg ctctgtttct gataataatc taagaggatc
     3901 cagattcatc tttgtcaggt aggggctatt tggtgaagca tatatttttc tgacaagaaa
     3961 gccatctttt cccggctcat cgataactat aaaaaagaaa cctcttgcag gcattaaggg
     4021 tcctaatata tcgtgatttg aatcaggtat atcatttgtt cctactgtat tctcagatat
     4081 caccattttt ccagcgcctt aataccaaga agtacgttta ggttatcagc ttcttccgtg
     4141 aaaattcttt cacagcaccc ggcagaatca gtaaacccct tatagatatt ccctatgctg
     4201 tcttctatat agtaaggtac attagcaatg aattttccgt catcatcaat tactctaact
     4261 tgctcatcaa atttcgcaga tattacagga aacgccatca gctccggcgt cagcttcgcc
     4321 tttggtttac gcccgtaata tccggcctta acgttatatt ctccctgcgt tcccgcttct
     4381 atcccacagg catccagccg gatgtagctg ccaccgccgt taagggtgat tttcttcttc
     4441 gcggtgatct tgatctcatc ttccgtactg gtgatctcta tcgatttctg tgccaacagt
     4501 tccatcagat cgttctgtgc ctgtaccgag atcgccccct tgttggcaaa cagcttgatg
     4561 cccgctttac gcacaaacac gcttagcgcc tgtcccacgc cgataaacat atttttcacc
     4621 gcgccgacat cggcgttgtt gcctgcgttg gctatcacat tctttgccgc cgccagttgc
     4681 aggtgctcgc cgctggcgat cgccacgccc ttgggtgcgc ttagcaggat cgcagcctgg
     4741 ttcagatctt ttacgctctg ttgcaacaac gcaatctgcg atgacagatc tgccggatcg
     4801 gcattcgccg tctgcgcatc agtggacaga gacttcatct gtgactgtgc gccattcagt
     4861 atcgccattg caggagccat ttcgcgcacc tgcccctgcg cattatcctg cgcatcggcg
     4921 ctgataaaga tgcctttctt tgcccgcacc acgccccaca tatcggtacg cagttcaaag
     4981 ccttcgccgc gttgctgttt cccggcatcc accagatgac cgaggtttag ctggctcttg
     5041 ccgccgtact cggtactgac cttgatatgt tctttgccgc gctcatcgtc gaggcggatt
     5101 ttgttgttcg ccggggttct gaggacgtta cgcttatagt tctgaatggt cacatggtca
     5161 gtgtgtgccg agtcgtgttt tacgcctgca atataaggtc ggtccgggtt gccttcctca
     5221 aaggcgatac tgacttccgt tcccgccagc agcggcaggt gcaggccgta ggtgtctccc
     5281 gcatacgggc gcgactggcg
//
LOCUS       DAZGTJ010000182         5285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000182 DAZGTJ010000000
VERSION     DAZGTJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.182"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            118..1476
                     /gene="mltD"
                     /locus_tag="ACHK8E_004978"
     CDS             118..1476
                     /gene="mltD"
                     /locus_tag="ACHK8E_004978"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP0347098.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1523..2293)
                     /locus_tag="ACHK8E_004979"
     CDS             complement(1523..2293)
                     /locus_tag="ACHK8E_004979"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0347099.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2370..3170)
                     /locus_tag="ACHK8E_004980"
     CDS             complement(2370..3170)
                     /locus_tag="ACHK8E_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0347100.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3411..4325
                     /gene="yafC"
                     /locus_tag="ACHK8E_004981"
     CDS             3411..4325
                     /gene="yafC"
                     /locus_tag="ACHK8E_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP0347101.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4322..5125)
                     /gene="dkgB"
                     /locus_tag="ACHK8E_004982"
     CDS             complement(4322..5125)
                     /gene="dkgB"
                     /locus_tag="ACHK8E_004982"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP0347102.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1383 a         1278 c         1316 g         1308 t
ORIGIN      
        1 tgaagaacgt tttgcatggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt
       61 catcggctcc gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg
      121 aaggcaaaag cgatattact cgcctctgtc ctgctcgtgg gttgccagag taccggcaac
      181 gttcaacagc acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca
      241 agtcaggcac gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct
      301 ttcattggcg acgagctaaa gatgggaatt ccggaaaatg accggattcg cgaacagaaa
      361 cagaaatatt tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat
      421 atgtactgga tagccgggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta
      481 cccatagtgg agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcaggcatc
      541 tggcagatca ttccgagcac ggggcgcaat tatggtttga aacagacccg caattatgac
      601 gcgcgtcgcg atgttgttgc ttcaacaact gccgcgctga acatgatgca gcgtctgaac
      661 aaaatgtttg acggcgactg gcttctgacc gtagctgctt ataacagcgg cgaaggtcga
      721 gtcatgaagg caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta
      781 ccgttgccgc aggaaacgaa gcagtacgtg cctaagatgc tggcattgag tgatattctc
      841 aaaaacagca agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggca
      901 cgtgtgcacc tgagcagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc
      961 gtcagcaagc tgaagacatt caacgcaggc gtgaaaggct ccacgctggg cgcaagtggt
     1021 ccgcagtacg tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctggcttca
     1081 ggcgaaattg ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt
     1141 gtttacaccg tacgctctgg cgacacgctt tcaagtatcg cttcacgtct cggcgtaagc
     1201 accaaagatt tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagt
     1261 ttgacgattg gtgcaggcag tagcgcacag cgactggcaa acaacagcga tagcattacg
     1321 tatcgtgtgc gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc
     1381 aaagatgtaa tgcgctggaa cagcgacact gcgaatctgc aaccaggcga taagctgacg
     1441 ttgtttgtga aaaacaacaa catgccagat tcctgacaaa ccagataata aaaggcaccg
     1501 attcccccgg tgcctttttt atttatgccg ctttatgtgc ttctaccatg atgatatcac
     1561 tggtgaaaga gccatcattc tgcaaggcaa aatacgtttt cacctctgtc gatgcgctct
     1621 gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc gcgacccatg
     1681 aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcat
     1741 tgattaacgt caaccactca ccgctggcgt agtttcgtac gtgagaggta tcgcgtaatg
     1801 cttctaccgt ctgtaaccag acgtcgcgca ctgggtgacc cggagacatt acgtccatca
     1861 caatcagcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct gcaccaacat
     1921 catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg
     1981 gcagactttc ggcatatccc tggcgggtgg cgatattttt caggtgccgg gcttcggcag
     2041 cttgtgccac gacatccagc atttgggcag ataagtcata cgccaccacc gcgctcacgt
     2101 tctgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt acacttgcat
     2161 caggataatc agccagacgc accgccaggc gctgtaaatc tctcccggat gcatgtacgg
     2221 tactggttaa atactcactg gcctgcgagc taaactgctt ttctacgtgg tcatggtggg
     2281 attgtgttgt cattgcgctg tccttttatt gtttgattaa aaagggcagc gccaaacgtt
     2341 ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg actgaattca
     2401 acgagtagcg gattgtgatc ggaagcgcgc gtaaccagta cagaagcttc gctgacgttc
     2461 agaccacggt agaaaacaaa atcgagcggg cggccaaacg cccgacggcg ctgatcatcg
     2521 gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc gttcatcctt
     2581 ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccgctgtg gtgagctatc
     2641 tgatcgccaa taggaagtaa ctgcttacta tagacatcca cgcccaaact gaaattgacg
     2701 gcgtgtatat taaccaccat caacaggcgg gtgtcaggca atggatagac cgtcaccagt
     2761 gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca atacactgga
     2821 tgcgccgccg aaagggtcat tacaccggaa ggatgttgtg gcagcacgaa agcgggtacc
     2881 tgatcggcgg caagatagtt agcggtcgca aactgcacta actctggcgt tgtctgcgct
     2941 tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga caaccattca
     3001 gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc ggtacttaac
     3061 ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg taagatcctt
     3121 tccgcaggtt gtccagcaac atagcgcatg gcataggtgt tttttcgcac ttttgacttt
     3181 gaaacctcta ttacgtcaac ccttccgcac tcgggagggt tctactgtta tagggatttt
     3241 agctcactct ttcaacgagc aattgtatat tgctatgtaa gcaagtgctt acaccggtca
     3301 gaaatttgct cattattgag aacattaaat cggatgacat atctcgcatc ctgatggttt
     3361 aatgacaatt aaattcaaaa tatctcttct attttgcaac aggagcaaaa atgaaagcca
     3421 cgtcggaaga actcgccatt tttgtttcag tcgtcgaaag cggcagcttt agccgggcag
     3481 cggaacaatt agggcaagca aactcagcgg taagccgggc ggtgaaaaag ctggagatga
     3541 aacttggcgt tagcctgctt aatcggacca cgcggcaact tagcctgacg gaagaaggcg
     3601 agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca gaatcagaaa
     3661 ttatggagac gcgtaataca ccgcgtggac tgttacggat cgatgccgca actccagtgg
     3721 tgctgcactt tctgatgccg ttgattaagc cttttcgtga acgctatccg gaagtcactt
     3781 tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg gatgtcgcga
     3841 tacgcgctgg tacgttaacg gattccagct tacgtgccag gccgttattt aacagttatc
     3901 gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa acgatcgacg
     3961 atttaaagca acatgtttgc ctgggattca ctgaacccgc ttccctcaat acctggccga
     4021 tagcctgtag tgatggacaa ttacatgagg tgaagtacgg tttgtcatcc aatagtgggg
     4081 aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc gactacatga
     4141 tcgacagaga aatcgctcgc ggagaattgg tggagttaat ggcagataaa gtattgccag
     4201 tggaaatgcc attcagtgcc gtatattaca gcgaccgtgc ggtaagtacg cgcatccggg
     4261 cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct gtcagagagg
     4321 cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg caatccagtg
     4381 cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta agattacttt
     4441 ccaggttttt acgtttagta gaagaaggaa ttacggagta accttccccc atagcccacg
     4501 ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc gatacgagca ataacctcat
     4561 ctttcagggc cttaccatac gccagcgtca tataggaagt aatatggatg ccgtgctgtt
     4621 tagcccaggc gaccacttta cggttttgca gataaggaga gagttcaatc tggttagtag
     4681 cgatgttttc agcgccaaca gcagcaatcg ccttttccat caatgggatc gtgaagttgg
     4741 aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc tgcataaact
     4801 cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga tcaacataat
     4861 cggtacgcaa tttttgcagg ctctctttca gactcgggat caatttgtct ttgctgagat
     4921 tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca ctttctgcaa
     4981 tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt gcgcgataac
     5041 caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga cggaaagtac
     5101 ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt cgttaactga
     5161 ggtgattatg ggcttcagac tgttgcggaa aaagagcgta aaacgcagag gatttttgcg
     5221 attctggcaa taatagaaat acagacgaaa aaaacctcga gcgttaactc gaggcttctt
     5281 aataa
//
LOCUS       DAZGTJ010000183         5282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000183 DAZGTJ010000000
VERSION     DAZGTJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.183"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1248)
                     /gene="ybdG"
                     /locus_tag="ACHK8E_004983"
     CDS             complement(1..1248)
                     /gene="ybdG"
                     /locus_tag="ACHK8E_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP0347103.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(1356..2009)
                     /gene="nfsB"
                     /locus_tag="ACHK8E_004984"
     CDS             complement(1356..2009)
                     /gene="nfsB"
                     /locus_tag="ACHK8E_004984"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP0347104.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(2103..2471)
                     /gene="ybdF"
                     /locus_tag="ACHK8E_004985"
     CDS             complement(2103..2471)
                     /gene="ybdF"
                     /locus_tag="ACHK8E_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0347105.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            complement(2536..2784)
                     /gene="ybdJ"
                     /locus_tag="ACHK8E_004986"
     CDS             complement(2536..2784)
                     /gene="ybdJ"
                     /locus_tag="ACHK8E_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP0347106.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(2850..3968)
                     /gene="ybdK"
                     /locus_tag="ACHK8E_004987"
     CDS             complement(2850..3968)
                     /gene="ybdK"
                     /locus_tag="ACHK8E_004987"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP0347107.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            complement(4173..4441)
                     /locus_tag="ACHK8E_004988"
                     /pseudo
     CDS             complement(4173..4441)
                     /locus_tag="ACHK8E_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4485..4930)
                     /locus_tag="ACHK8E_004989"
                     /pseudo
     CDS             complement(4485..4930)
                     /locus_tag="ACHK8E_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
BASE COUNT         1437 a         1249 c         1277 g         1319 t
ORIGIN      
        1 ttactgctta aatgcacccg ccagagagcg aatatcattg ccagttggcg actgatgaag
       61 tcgcagacca aactcttcga caatcgcaaa tatgtggtcg aatatatcag cctgaatgct
      121 ttcatattcc agccacacca cggtgttggt aaacgcatag atctcgagcg gtaaaccgtt
      181 atcgccagga gccagttggc gtaccattaa ggtcatatct ttacgaatcc gcggatggtt
      241 acgtagatat tcgttcagat aggcacgaaa ggttccaata ttggtcattc ggcgcaggtt
      301 taataccgac tccgtagaac cttgctggcg attccactca ttaatttcct gatggcggct
      361 ggttaaataa ggctttaaca aatgcgcttt attcagacgt tgcatttcgt cttcatcaag
      421 aaaacgaata ctggtgacat caatactgat actgcgctta atacgtcgcc cgccagatgc
      481 tgacatcccg ctccagtttt taaaggagtc agaaaccaga gaccaggtgg gaatagtggt
      541 aatggtattg tcccagttac gtactttgac ggtggttaac ccaatatcga tcaccgcgcc
      601 atccgcgccg tatttcggca tctccagcca gtcgcccagt ttcagcatat cgttcgcgga
      661 aagctgaata cctgccacca gaccaagaat cggatcttta aataccaaca tcagcacggc
      721 agccattgca ccaagaccgc tgatcagaat cgctggcgac tgaccaatca gcagcgagat
      781 catcaaaatg ccgaccagaa tcgcgccgat cagtttaatc ccctgaaata tccctttcag
      841 cggtaactga gatgccgccg ggaatttctg cgccagattc aaaatcacat ccagcaacga
      901 gaagactgaa agcagcgcat acatcatgat ccacaactgc gcgcaggtag tcagaatatc
      961 tgccgcttcg gtgccttttt gcagccagaa taccgcctga atattgacga taatcccctg
     1021 cagggtaaaa gctaaacggt ggaagagttt attctgggta atgatttgca accaaagccg
     1081 tgaactggcg atggcgcgtt tttcgaaggt ccgcagtacc acccagtgca aaaaaatatg
     1141 caccacgacg gcggtcagaa aaataatacc gaaaatcatc accatcgagg tggtgtgatc
     1201 gatctcaata cccgctaaat cttcaacctg ggatattaaa tcctgcataa cgtctccttt
     1261 atacaacagc agcctatgat gacggctgaa acagggttat gcaaatcagg agaatctgag
     1321 gggaaatagc cgggcagatg cccggcaagg gagaattaca cttcggttaa ggtaatgttt
     1381 tgcggcagac gagatttcgg cagcgtagcg ttaaaatctt caacgctgtg atgacccacc
     1441 ggaacaacca ccagactggt gtagcctttc tcttccagac caaattcttc atcgaggatg
     1501 gcggcgtcaa aaccttcgat gggcaccgcg tccagaccca gcgccgccac gccgagcagg
     1561 aagttaccga cgttgagata aacctgtttt gccatccact ctgcatcatc gtgcagatct
     1621 ttacggtgca tatcggcgaa gaacttgcga cctttatcgt tcgcggcttt cgcttccggc
     1681 gtggcaaagc ggccatcggc atcttcctgg tcaacaacca gcttcagcca ggcatcgtcc
     1741 atcgcggttt ttgcacagaa caccacgacg tgcgaggcat caagcatttt acgctcgttg
     1801 aacacgtaat taccggcagc ggatttggca acacgcgctt taccttcttc cgtgctggca
     1861 acaataaaat gccacggctg ggagttggta ctggatgggc tgtattgcag gagagttttg
     1921 acctgctcgg cctgttccgg ggttagtttt ttgctggcat caaatgcctt agtggaatga
     1981 cgctttaagg cgacagaaat gatatccata aagactccat gtgaaagtaa ttttgcgtgc
     2041 cagcagatta caaggttcaa cgagaaatgg taagcgagaa aaatgcgcta tagatttccg
     2101 ctttagcctg gacgcactct tttttgatcg cgtttagcca gaccatcaac caccagattc
     2161 cacgaatcgt tgatcagatc gcgaagtaac gcttcggaga tttcctcgcc gggatacact
     2221 gaaatccagt gctttttatt catgtgatac cctggcttga tgctcgggta tatttgctga
     2281 tttaacaggg atttttgtgg atcggacttc agattgataa aggggacgcc gcgcagctcc
     2341 gacgacagca taaaaatctt gccgccaatt ttaaaaacat cgaactccgg gccaaaaggc
     2401 cagcaaagct cgacaaaggg taactcaagg gccaggcgtt tcgccgtttc gtgcagtgat
     2461 tgcttatcca taaacgttcc tttaggcgaa ggagaataag caaagtatgc cgcgaagtac
     2521 ggcgataatc gacgtttaat ccgccagcga gaaccagcgt cgccagataa agcgcagaac
     2581 aaaatactca atagcgccca gcactaaaaa ccacagacaa aacaataaag tgtaaagctg
     2641 gctaagatcc atcagatgga acatggtcac cagtttttgt gccagcgtca gccccagtgc
     2701 gggggcgggc agcagcaggc aagaaaggaa agccatcagc aaaatgcctg ctgcggtggt
     2761 caaggtttct aaagggtgtt tcataacatt gttaaatgta agttaaaaca ccattgtcag
     2821 ggatattctt ctgtaaggca attcccggct tagtcaccag cccagatctc acaatgcttt
     2881 ttcaccagcc caatcagcga gccgccatcg gcgacgaaat cgcgcatcag ctgcgcttca
     2941 ttcagaccgc tgacgacctg gcggtgcagt gcttctattg cgctcgatgc gccaatttta
     3001 tgtgcagaag gggcgatttt ttccagtaaa cgcaaggtgt cttccattag tgaccgacga
     3061 tcgccagtat gcggatcggt aatgacgcct tccagcccat agcgacaggc ctggaaacgg
     3121 ttgaatttat acagcaggta atctttctcc tgatgtttga acgggcgttc tgtcagtaac
     3181 cagtgggcag tagcctgaat taatcccgcc atatttaccg catggctaag ggttaacggg
     3241 gtatccatta cccgaacctc caccgtgcca aaatgaggac tggggcgaat atcccagtgc
     3301 agatctttaa tgctgtcgat catcgtggtg taactcaagc agcgaaacag ggcttcaaat
     3361 tgttgccagt tactgaccca cggcatcggg ccattatcgg gaaaggcgga aaaaatattc
     3421 ggtcgtgagg aggcaaaacg cgtatccgtt ccttgcatat atggcgacgc ggcggaaagg
     3481 gcgataaagt gcggcacaaa ccgtgacagg ccgtgcagca aataaatggc atcatcgcca
     3541 ctggcgcagc caacatggac atgctgacca aaaacggtcg cctgctggat gagatagcca
     3601 aagttttcca gcgttcgttg atagcgttcg ttatcgcata cctcctgacg ctgccatttc
     3661 tgaaacgggt gcgtgccacc gccgcaaatt tccagatgat ggtctgcggc tgcctgcaat
     3721 acgactttct gcatcgctga aaattgcccg gcagcctggt tgatatcacg gcaaacatcc
     3781 gtcgccagct ccagcatact ttcggtgata tcgtgcttta cctctccggc cgtgatctta
     3841 tttttaaccg cgtcaatcag cattgaagag tcctggctta agtcatagcc cggcggatta
     3901 accacctgca tttccagttc aataccgagg gtaaaaggtt cagagacttg aaaatcgggt
     3961 attggcatag gtttctctta tgttggcgtt ttctattcag tatagaagtc ggagcggctg
     4021 ggcgagatgc ggaagttctg gaatgtttct ttttttgatg atggtgactg aagcaaattg
     4081 gctacatttt gcaatatgac aagttatggc agtgcggact ggtggcgaag aattttgacg
     4141 attgaggcat gcagaataaa acgggttcag cttcagttga tcaaatgagg attgagaagt
     4201 ccgaaacagg acttactgta taaataaaca gccattttgc taaggaaggg taagataacg
     4261 gcgggtgcct gaagctttcc ggtttcaggt ttactctgag gtctggaaag atgaagcccc
     4321 aggagatatt tctatcaatc ctggggctgc cactccaaac ccaaacaatt tggatggtag
     4381 tcccttcttt gcatggaggc aatataaaca tgctgacgaa atatgccctt gtggcggtca
     4441 tggtgctgtg tttaaaggta atttttatcc ggtttattcg gagcaatagt gattgcaagc
     4501 gtatcaagaa aattcttata gaactgagca atttgttgcc agaatgacga tacaacctga
     4561 tccagaagta atatagaacg cccgtcgcaa acttggggag tatgcagaac atggaatgcc
     4621 tgagaaggat aaccactcca gtcaacggca atgacgcaca acgtaaggtc tcggggctga
     4681 aaccgggtaa tattatgaaa aatagccggg atatcgcggt gtaaagcgtc attgcctggc
     4741 aggcgatcga tgggcttaat cttgttttaa cctgagcatt gcctggcata taccgtccaa
     4801 tgctggtaag gatgagggat gcgtcccgac taagcgcagc agtggcgtca aggatggcat
     4861 tctggcggta ctgatgaaat ggagtcaaat catcacggaa aaacttttgg catgatgagc
     4921 gaacgggcat agaggtgatc tcctgaattg ttagcacaac cagtcgacca caaaactcta
     4981 tgcccgtctt ttttacccgc ctattactga ggatttctcc gtaccattac cggcatttgt
     5041 tatctttcat tgctacatac gtcatccccc aacaaagcaa tgctttataa tattatcgta
     5101 atgtcttatt ttttattatt gcgatggctt tctgaacatt ttatagtgtt aggtgtaaat
     5161 catttatatt gattcacgat aggaggatat ggacaccaca cttattttat gtcactcaag
     5221 aaacacttaa aattcgttgc acgtgttatt attctattaa ataattgcac ataatagatt
     5281 tt
//
LOCUS       DAZGTJ010000184         5212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000184 DAZGTJ010000000
VERSION     DAZGTJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.184"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            211..699
                     /locus_tag="ACHK8E_004990"
     CDS             211..699
                     /locus_tag="ACHK8E_004990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347108.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTKKTLTEITNALDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISGLYMKNLRLERELAELRAQMKTDESPTEEINARPE
                     HTNAHITMITRKAERTHICELLRELIETAKETHTCPQETCAAIERLIAMLTNPDELKG
                     RA"
     gene            699..1280
                     /locus_tag="ACHK8E_004991"
     CDS             699..1280
                     /locus_tag="ACHK8E_004991"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0347109.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNERCMRPLIDMAESTGWRMPGRQG
                     KETIAHTALNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            1318..2292
                     /locus_tag="ACHK8E_004992"
     CDS             1318..2292
                     /locus_tag="ACHK8E_004992"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP0347110.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRTARDNTDSLIDEAKDLFREHNNRTDYLAIRNTFNA
                     ENHAFLSTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPFNARDNFTAYG
                     KPFTKDEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCDACGRHGGGYCPDCGPVTGYETYSAIFATANT"
     gene            2328..2564
                     /locus_tag="ACHK8E_004993"
     CDS             2328..2564
                     /locus_tag="ACHK8E_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347111.1"
                     /translation="MNSERNKTLLQGLRHIAHAHEETAKLATAVGKPTVVISSAISQG
                     ITIEAIQVIQDQEREIAALKKLLHVKSAGQGVKQ"
     gene            2561..2767
                     /locus_tag="ACHK8E_004994"
     CDS             2561..2767
                     /locus_tag="ACHK8E_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347112.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSHFRATNVEEVRVLTTSCRRAL"
     gene            2793..3173
                     /locus_tag="ACHK8E_004995"
                     /pseudo
     CDS             2793..3173
                     /locus_tag="ACHK8E_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3104..3331
                     /locus_tag="ACHK8E_004996"
     CDS             3104..3331
                     /locus_tag="ACHK8E_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0347113.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            3400..3816
                     /locus_tag="ACHK8E_004997"
     CDS             3400..3816
                     /locus_tag="ACHK8E_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347114.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMALVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            3813..>5212
                     /locus_tag="ACHK8E_004998"
     CDS             3813..>5212
                     /locus_tag="ACHK8E_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0347115.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEGKRINSHFHHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERTCDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDSRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRAINKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSPENEARITE"
BASE COUNT         1377 a         1487 c         1346 g         1002 t
ORIGIN      
        1 gcagcatgag catatttctt tcatgggtga ttctgattat ttccgtgctt atggcgattg
       61 gcattatgcg aattatcgaa gcgatgaaaa atatcgaatc tttatttact ggcgaagaac
      121 gccgtaaata aataaccaac cggacagaaa acattaaaac catccgcatc tgcggagggc
      181 ttcactcaat ccagaaacgg agaaacagct atgagcacca atacacctac agaaataaac
      241 aaactattta ccgacccagc gcatattgaa ttgacgaaaa aaacgctgac tgaaataacc
      301 aatgcactgg atgaaagaat atcagaaata gataaagaca agcattttgc cacctacatg
      361 gccctgcaaa tgcaaagcat ggcaatggtg acagcaaaac agaccatcag tggactctat
      421 atgaaaaacc tgcgcctcga gcgcgaactg gcagaactcc gggcacaaat gaaaacagac
      481 gaatccccca ccgaagaaat aaacgccaga ccggaacaca cgaacgcgca tatcaccatg
      541 atcacaagaa aagccgagcg tactcatatt tgcgaattac tgcgcgagct gattgagaca
      601 gcgaaagaga cacacacctg cccacaggaa acctgtgcag ccattgaacg actgattgca
      661 atgctcacca acccggacga actgaaggga cgcgcataat gaaccatctc atgattgaca
      721 ccgaaacact gggtaacgga ccggacgcag ccatattcgc gatcggggca gtgtttttcg
      781 acccgttcac cggaaagctg ggaaaacagt ttgagcgatt cattgacccg gtggattcag
      841 agcgcaacgg tggaaccgtc aatgcagcaa ccgctgtatg gtgggccgga caatccgtgg
      901 acgccagagc ctgcctgcgc aacgcagatg gaacagagct ggcagcagtg accgaattcc
      961 tggcgttcat ttcacgcaat atgcatgacg aaagcccggg caagacactg accgtatggt
     1021 gcaagggcgc ttcgtttgac ttcccgatcc tgaaatccgc tatcacgcga acaaccggag
     1081 ataaaagcat cccctggtgt tactggaacg agcgctgtat gcgtccgctt atcgacatgg
     1141 ctgaatcaac aggctggaga atgccaggcc gacaaggaaa agagaccatt gctcataccg
     1201 cactgaacga cgccatttac caggcgaagg ttgtttcaga aatctggcag cgcttcactg
     1261 cgccattcct gagcgtatga agcggacaca cgaaacctgt aacaaagagg ccgcgttatg
     1321 agcaaagaac tgttatcagg cacaccactg aaatgggtgg gcggaaaaag ccgcatcatg
     1381 aacacactga aggcgcacct gccagaagcc gactgtctgg ttgagccgtt cgtgggcggc
     1441 gcatcggtat tcatgaacac cagttacagc cgctacatcc ttggagattc caatggtgcg
     1501 ctgattaatt tctggcggac agcacgggac aacaccgaca gtctgatcga cgaggcaaaa
     1561 gacctgttcc gggaacacaa caacaggact gactatctcg ccatccgaaa cacctttaat
     1621 gcagagaatc atgcattcct gagcacggga ataagcgacc gtcgggaact gcgactggcc
     1681 gccatgtttt tgtatctcaa ccggcactgc tttaatggcc tgtaccgggt gaacggaaaa
     1741 ggtgacttta acgttccttt cggtggctac cgaactccct acttccctga acgggaaatc
     1801 cgcgcatttg cagacaaggc aaacagcacc cgaacactgc ttgttcatgg cgattttcga
     1861 aaaacactga gctgtgccag ccacctgttc agcatgggta acacgctttg cgtgtactgc
     1921 gatccccctt acctgccttt caatgcccgg gacaatttca ccgcctacgg aaaacccttc
     1981 acgaaggacg aacacgttcg cctgagagca gcgcttgacc gtctttcaca ggaaacgggc
     2041 ggcatgacca gcatcaccat atccaattcc gacacaccgg aaaccagacg tatttatcag
     2101 ggttaccgga tgaactgcat acaggcacca cgaagcgttg gcgcaagaac ccgcgaacca
     2161 gcaccggagg ttatcgccac cctgaaacag tgcgacgcat gcgggcgcca tggcggcggc
     2221 tactgcccgg actgcgggcc ggtgacggga tatgaaacgt acagcgcaat atttgccact
     2281 gccaatacgt gaataaacag atgaatcctt aacaccggag tcaaccaatg aatagcgaaa
     2341 gaaacaaaac tcttttacag gggttacggc acatcgccca tgcacacgag gaaacagcca
     2401 aacttgcaac tgccgttgga aaaccaacgg tcgttatctc ttcagcgatt tcacagggga
     2461 tcaccataga agcaattcag gtaattcagg atcaggaaag agaaatcgcc gcccttaaaa
     2521 agctactcca cgttaaatct gccgggcaag gagtcaagca atgatcaagg agtcaaccgt
     2581 tatcagattc acaacccacg gccatcagta cgaagtcgac gaaagcctga ttgatcaggg
     2641 catgacacgc cgggattcac gcaatcccga tatatatcac atccgcctta tcaacggttc
     2701 gcacttccgc gccacgaacg tggaagaagt acgcgtgctt accacaagct gccgtcgtgc
     2761 tctgtgaact gttaccggac tgagtgatca tcatgcacac acaaaaaaac cgcttgccag
     2821 cgttcgcctg gtcgggttac agttttcccg cacctcataa aacgggtgcc gggatttcca
     2881 ccccgccgat aaccaaagcg cacaaccgcg ccagcggttt ttttgtgcgt actgtattgc
     2941 cacgtctttt tcgcgtcaga attatggcgg ggcgtacggg gccgacttcg gtcgggccgg
     3001 gttctttggt taccggttgt ggaaaccctg tacgcctcgc caccccgagt tttccacctc
     3061 tggatggtga gttttcaaaa ttcacaacca aagaggccac accatggcaa accgcaaaca
     3121 acagcgcgcc cgcgctgagc gtcttcacat ccgcactgaa attaaccgca gactccatcg
     3181 ggctgcatct gtcaccttca tcatgcagtc aaacgcatta catcgactta acgatggtat
     3241 cgctgccggg tattacgcgg cagtcttcag ctatctggcc gaagacctgg cagaactgca
     3301 ggaccttatc aaccagcaac accaccacta aacaacatcc tgaagccatc ccgcacccgt
     3361 gcgcgggagg cttccgcacg tcggttaccg gaggttctca tgaagagcgt taagggactt
     3421 tcagatattc aggatttttt taactggatg tgggcagtgg atccccggct ggcttcccgc
     3481 ctgaacgact ggcacgacta ttaccgccgc gcctgtaaac aggccgccag acgcgtcacc
     3541 acatacgggg atttctcctt caccgttgac gaccgttacc gcgtggacgt gaaaggcgat
     3601 ggcgatggct ttggtttatt tcacatcaac ggcaccgaca gcaccgccat cagctggtcg
     3661 tcgtcagaga tggcgcttgt gctggaaatg acaacgcaca gcgtggccgt gaaaggcata
     3721 tacaccgcag aagactttat cggtgaatac cagcgccttc tcgtgctgta ccgcgcggcg
     3781 tttcaggcgg cgactgccgg ggagtgcgac gcatgagtga ccacaatgca cagctggcgt
     3841 acccgtggaa cgccccgcta ccggttatcg atcctgaaac gttcggcaga gcgcaaccaa
     3901 ccgcattacg ccaggccatt cagagctaca tcaacgaaga cattcgcatt gatgcaagac
     3961 tggatgaaga gaccgtcgac ttcctgacaa acacagacga aggcaagcga attaattcgc
     4021 attttcacca cgacgaagag cgtcggatca ggctggaaaa actggcgcgg cacaaacacg
     4081 agaatccgcc aacactggta tctgaagcca tggcagagct gcgcgccctt ccgccgtttc
     4141 ttgccggtcc gctcatccgt gacctggcac gcctgagccg caaacaggag agcgcacgcc
     4201 gggaagggct taagaacaac agcgcatacg cggcggataa cttcgttcgt cacgggctgc
     4261 gtgcccgcct gaaacgtatc gcccgtgtga atgaacgctt tgccaccccg gcctttaagg
     4321 ccaccgccgc gcgcgaacgg ctggatgaac tgcttatgct gccgcagctg agccgtgacg
     4381 aaatccagcg ccttgccacc ctgaccgcca ccgcgttttc cgccgcactg gagcgcacat
     4441 gtgatgaggt catcgaaaag accggcaggg gtgacgatga cctgttcacc tggttactga
     4501 cctaccagcc actggcacac atggcgttaa aactgggtat cacgccaccc tgctggccgt
     4561 cgctggaaat caggcccgac agccgcaccg cccccgaccc ggaacaggtg ccgggtgcgg
     4621 tcatgcgcct ttcctgtgcg acatggtgga acagtcagtt aagacacctc cgggacatct
     4681 ggcgtgaaga gctgttacgc gccgcaggcc tggtgtcacg taagacgtcg gtatatatca
     4741 gccatgaagc gttactggac tggcgcgaaa aacgccagcg cacccgcgat tttctgaagg
     4801 cctgggacat cgagaacgaa gaaggagaac ggttcagcct tgaggatatc tactgggcag
     4861 gtctctctaa tccgcgtaac cgccgtaacg agatgatggc ctgcgtccgg ggaatggagc
     4921 aggtcgccga agcacgcggc gacgtggcgt ttttcgtcac cgtcacagcc ccttcccgtt
     4981 ttcacagcgt gaacgatgac ggcagtctga acccgaaata ctgcggggca acggtacgtg
     5041 atgccagtga ttatctggtt taccgctttt tcgccgccgt ccgcagggcc atcaacaagg
     5101 ccgggctggg ctggtacggc gtgcgcaccg tggagcccca ccacgacggt acaccgcact
     5161 ggcacatgct ggtattcacc agcccggaaa acgaagcccg tatcactgaa at
//
LOCUS       DAZGTJ010000185         5209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000185 DAZGTJ010000000
VERSION     DAZGTJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.185"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..129
                     /locus_tag="ACHK8E_004999"
     CDS             <1..129
                     /locus_tag="ACHK8E_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0347116.1"
                     /translation="VAVMPHAAKVLKQVMVNTGGNKEQVPAKRQNRGSRNSTDDGG"
     gene            155..676
                     /locus_tag="ACHK8E_005000"
     CDS             155..676
                     /locus_tag="ACHK8E_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0347117.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            734..967
                     /locus_tag="ACHK8E_005001"
     CDS             734..967
                     /locus_tag="ACHK8E_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0347118.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1028..2986
                     /locus_tag="ACHK8E_005002"
     CDS             1028..2986
                     /locus_tag="ACHK8E_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0347119.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQTVAEHIREAEGWEWCAGRMEPVGFCREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQSEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMLQPE
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            3041..3475
                     /gene="psiB"
                     /locus_tag="ACHK8E_005003"
     CDS             3041..3475
                     /gene="psiB"
                     /locus_tag="ACHK8E_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0347120.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGESFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVSDIIHILMVEGGQV"
     gene            3472..4191
                     /locus_tag="ACHK8E_005004"
     CDS             3472..4191
                     /locus_tag="ACHK8E_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0347121.1"
                     /translation="MSVRSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4188..4502
                     /locus_tag="ACHK8E_005005"
     CDS             4188..4502
                     /locus_tag="ACHK8E_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFP0347122.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVCRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4651..4800
                     /gene="mok"
                     /locus_tag="ACHK8E_005006"
                     /pseudo
     CDS             4651..4800
                     /gene="mok"
                     /locus_tag="ACHK8E_005006"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4742..4867
                     /locus_tag="ACHK8E_005007"
     CDS             4742..4867
                     /locus_tag="ACHK8E_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP0347123.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGDREVAAFLAYESGK"
BASE COUNT         1169 a         1351 c         1631 g         1058 t
ORIGIN      
        1 gtggcagtga tgccccatgc ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg
       61 aacaaagagc aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacagatgat
      121 gggggctgaa aacatcaaca gaaggagaca catcatggca gttcgtggca ttaacaaggt
      181 cattcttgtc ggtcgtctgg gaaaagatcc ggaagtccgt tacatcccca acgggggcgc
      241 agtggcaaac ctgcaggtgg ccacgtctga aagctggcgc gacaaacaga cgggggaaat
      301 gaaggaacag acggaatggc accgcgtggt gctgttcggc aagctcgcgg aagtggcagg
      361 tgaatatctg cgtaagggcg cgcaggtcta catcgagggg caactccgta cccgtagctg
      421 ggaagataac ggtatcaccc gttacgtcac tgaaatcctt gtgaaaacca cgggcaccat
      481 gcagatgctg gggagtgcac cacagcagaa cgctcaggca caacctcagc ctcagcagaa
      541 tggtcagcca cagagtgctg acgcgacgaa aaaaggtggc gcgaaaacga aaggccgtgg
      601 acgtaaggcc gcgcagccgg agcctcagca gccgtctgaa cccgcgtatg acttcgatga
      661 cgatattccg ttctgaacgg gctgactgtg ataaccgccc cgtcctgcgc ggggcatcac
      721 cggagagatg aggatgagcg aatatttcag aatacttcag ggactgccgg acggtccttt
      781 tacccgcaaa caggccgaag ccgttgccgc acagtaccgg aacgtcttta tcgaggatga
      841 ccacggcgaa cagtttcgcc tggttgtccg taataacgga acaatggtct ggcgtacctg
      901 gaattttgag gacggtgcag ggtactggat gaaccatgtc atccgtgatt tcgggatcct
      961 taagtaagag aggtgccgga cggggatcac gtccggcaga cattaactac acaataaggg
     1021 gatagtcatg tcagtaacaa agtctgaacc aaaatctacc cgtaaagcaa gccgtaaacc
     1081 tgcaaaaacg caggaaactg tcctgtctgc cctgctggcg cagacggagg aagtgagcgt
     1141 gccgctggcc tcgctgatta aatcacctct gaatgtgcgc acggtgccgt attctgcgga
     1201 gtccgtcagc gaactggcgg agtcaattaa aggtgtcggc ctgctgcaga atctggttgt
     1261 tcatgccctg ccaggtgacc gccacggtgt cgccgcaggc ggtcgccgac tggcagcact
     1321 caacatgctg gcagagcgtg gcatcattcc ggctgactgg cctgtccgcg taaaaattat
     1381 tccgcaggaa ctggcgacgg ccgcatcgat gaccgagaac ggtcatcgtc gggatatgca
     1441 ccctgccgaa cagattgccg gattccgcgc aatggcgcag gaaggcaaaa cacctgcaca
     1501 aatcggtgat ttgctgggct attctccccg ccatgtccag cgaatgctga aactggcaga
     1561 ccttgcgcct gtcatcctcg atgcgctggc agaagaccgc atcaccaccg aacactgtca
     1621 ggcgctggcg ctggagaacg acaccgcgcg tcaggtgcag gtgtttgaag ccgcctgcca
     1681 gtcaggatgg ggcggtaaac cggaagtgca gaccattcgt cgtctggtga ccgaaagtga
     1741 agtggcggtg gcggggaaca gtaaattccg cttcgtgggg gctgatgcct tctcgccaga
     1801 tgaactgcgc actgacctgt tcagcgacga cgagggtggc tatgtggact gcgtggcgct
     1861 cgatgccgcc ctgctggaaa aactccagac tgtcgctgaa cacattcggg aagccgaagg
     1921 ctgggaatgg tgcgccggac gtatggagcc tgtcggtttc tgccgtgagg atgccggaac
     1981 ataccgttgt ctgccggagc cggaagcggt gctgacggag gcagaagaag aacgcctgaa
     2041 cgaactgatg acgcgttacg acgcgctgga aaaccagagt gaggaatccg acctgctgga
     2101 agcagaaatg aagctgatgc gttgcatggc gaaggtcagg gcgtggacgc cggagatgcg
     2161 tgccggaagc ggtgtggtgg tgtcctggcg ttacggtaat gtgtgtgtcc agcgtggtgt
     2221 gcagttgcgc agtgaggatg acgtggctgc cgacgctgac cgcacggaac aggtgcagga
     2281 gaaagcgtca gtggaggaaa tcagtctgcc actgctgacg aaaatgtctt ccgaacgcac
     2341 gctggcagtc caggcggcac tgatgctgca accggaaaaa tctctggcac tgctggcatg
     2401 gacgctctgc ctgaatgtgt ttggcagcgg agcgtacagt aaaccagcac aaatcagcct
     2461 ggaatgtaaa cattattcgc tgaccagcga tgcgccatcg gggaaggaag gtgccgcatt
     2521 catggcgatg atggcagaaa aagcccgtct tgcagccctg ctgccggagg gatggtcacg
     2581 ggacatgacg acgttcctgt cactcagtca ggaggtgctg ttatccctgc tcagtttctg
     2641 caccgcgtgc agcatccacg gcgttcagac ccgcgagtat ggtcacacgt cacgcagtcc
     2701 gctggactcg ctggagagcg ccatcggctt tcacatgcgt gactggtggc agccgacaaa
     2761 agcaaacttc ttcggacacc tgaaaaagcc gcagattatc gatgccctga atgatgccgg
     2821 actgtccggt gctgcgcggg atgcggagaa gatgaagaaa ggtgatgcgg ctgaacatgc
     2881 agagcaccat atgaaagaca accgctgggt tccaggctgg atgtgtgcac cacatccaca
     2941 gacagatgcc actgaacgca ccgataacct ggctgatgcc gcctgatgaa caaccacacc
     3001 gccccgccgg agacggggcg gcagcaaggg agatactgtg atgaaaactg aactgaccct
     3061 gaatgtatta cagaccatga gtgcacagga atatgaagat atccgggctg ccggaagtga
     3121 tgaacgtcgt gaactcacac atgccgtgat gcgggagctg gatgcgccgg ataactggac
     3181 gatgaatggc gagtatggca gcgagtttgg cggctttttc cccgtccagg tccgtttcac
     3241 acctgcccac gaacgttttc acctggcgtt atgttcgccg ggtgatgtct cccaggtctg
     3301 ggtgctggtt ctggtgaatg ccggtggtga gtcgtttgcg gtggttcagg tgcagcggcg
     3361 gtttgcaccg gaagccgtca gccattcact ggcactggcg gcgtcactgg atacgcaggg
     3421 gtacagcgtc agtgacatca tccatatcct gatggtggaa ggaggtcagg tatgagcgta
     3481 cgttcacagg cactggttcc cctcagcaca gagcaacagg ccgcatggcg ggcggtggcg
     3541 gagacagaaa aacgccgcca tcagggtaac acactggcgg aatatccgta tgcgggggcg
     3601 tttttccgct gtctgaacgg aagccgccgg atttcgctgt ctgacctgcg atttttcatg
     3661 ccgtccctga ccgcagaaga actgcacggc aaccgtcttc agtggctgta tgccatcgat
     3721 gtgctgattg aaacacaggg cgaggtctgc ctcctccccc ttcccggcga tgcagcagag
     3781 cggttgttcc cgtcggtgcg gttccgtgtc agggaacgcc gtcgccataa atctgcgctg
     3841 gttatgcaga agtacagccg ccagcaggca cgggaggcag agcagaaagc ccgtgcgtac
     3901 caggcgctgg tcgctcaggc tgaaatcgaa ctggcgttcc attcgccgga aacagtcgga
     3961 agctggcatg cacgctggag tgaccgggtg gcagagcatg acctggaaac cctgttctgg
     4021 cagtggggtg agcgttttcc gtcactggcc ggaatggaac gctggcagtg gcaggatatg
     4081 ccgttctggc aggtcatcgc agaggcaggt atggcagcaa gagaggcggg tcacgctgtc
     4141 cgtgagatgg agcggtggat ggtgccgaac aaactccggg aggcggcgtg aagtggcgtt
     4201 actcactgcg ctggaaactg ccttctccct gtcctggcga gcatgaactg gtttcagaag
     4261 tggtggaggc ggggcagcct gcaccggcat ccgttatgtc acgctgggtt gccggagcag
     4321 ggtatgcggt ctgtctggat tttatttctg accgcccggt gtgccgctgg agcgaagaac
     4381 gtaaggctgc ggtacggcgg cggaatctgg agaaacgtat caacaggcat gcaccactgt
     4441 ttgcggatga actgattgcc cgtgaactgg ctgaacgacc ggattatttt cagggaaagt
     4501 gagtgtggtc agcgcgcagg gatatgggct atgatgtgcc cggcgcttga ggctttctgc
     4561 ctcatgacgt gaaggtggtt tgttaccgtg ttgtgtggca gaaggacaaa agccccgtag
     4621 ttaattttca ttaacccacg aggcccctgc atgcctagac aacatcagga tagcctctta
     4681 ccgcgctttg cgcaaggaga agaaggccat gaaactacca cgaagttccc ttatctggtg
     4741 tgtgttgata gtgtgtctca cactgttgat attcacttat ctgacacgaa aatcgctgtg
     4801 cgagattcgt tacagagacg gagacaggga ggtggcggct ttcctggctt acgaatccgg
     4861 taagtagcaa cctggaggcg ggcgcaggcc cgccttttca gggctgatgc tggtctgact
     4921 gcactgaagc gcctttataa aggggctgct ggttcgccgg tggccccttt ctccttgctg
     4981 acattgtagc agcatgaaca accagactgc caggaaggcc acttccctcc gggaacggga
     5041 atgaacgtgc gcctgcggcg ctcgttcttt tcccccgcct tctctgttta tggccagttg
     5101 tcagaaatga cattaccttt cccgtgatta ttctcttttt cctcctgttc tgactctgat
     5161 tactgctgcc ctgattattg tctgttttgt tgttctcctg cttatgctt
//
LOCUS       DAZGTJ010000186         5191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000186 DAZGTJ010000000
VERSION     DAZGTJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.186"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            169..741
                     /gene="lpfA-O113"
                     /locus_tag="ACHK8E_005008"
     CDS             169..741
                     /gene="lpfA-O113"
                     /locus_tag="ACHK8E_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP0347124.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            789..1519
                     /gene="lpfB-O113"
                     /locus_tag="ACHK8E_005009"
                     /pseudo
     CDS             789..1519
                     /gene="lpfB-O113"
                     /locus_tag="ACHK8E_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            1544..4066
                     /gene="lpfC-O113"
                     /locus_tag="ACHK8E_005010"
     CDS             1544..4066
                     /gene="lpfC-O113"
                     /locus_tag="ACHK8E_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP0347125.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            4077..5147
                     /locus_tag="ACHK8E_005011"
     CDS             4077..5147
                     /locus_tag="ACHK8E_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0347126.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
BASE COUNT         1438 a         1111 c         1217 g         1425 t
ORIGIN      
        1 tgctgtttta ataatattta aataataatt tatatttaag attgagtgat taatattatt
       61 tttggataaa gttgtctctt tggtgtacta ttcctttgga tcaaaaatac acttattaat
      121 ataagtgatg attcatggta aaggatatat tatatcaatg gagtttttat gaagcgtaat
      181 attataggcg gtgcattcac tctggcatct ttaatgctgg ccggacatgc actggcagaa
      241 gatggtgttg ttcacttcgt cggtgaaatt gtcgacacga cttgtgaagt tacctccgat
      301 acagccgatc aaaccgtccc actgggtaaa gtcagtaaaa atgcattttc aggtgtaggt
      361 agtctggcgt caccacagca gttcagtatt aaacttgaaa agtgcccggc aacctacact
      421 caggcagccg ttcgttttga tggtacagaa gcgcctggcg gcgacggcga cctgaaagtg
      481 ggtacgccgc ttacagcggg caaccctggc gattttaccg gtacaggaca agcgattgcg
      541 gcaacgggtg tcggtattcg tatttttaac cagtcagata actcgcaggt taaactttat
      601 aacgattctg cctataccgc tatcgacgct gaaggcaagg ctgaaatgaa gtttattgca
      661 cgctatgtgg caaccaatgc aaccgtaacg gcgggtacgg cgaacgccga ttcacaattt
      721 actgtcgaat ataagaaata atttatcaag ggccttttta ttcagttggc tttggccagg
      781 gagtcgttat gtccagaaaa agttgtttaa tcaggctctt taaaacaacc gtttcatgta
      841 agttatttac cgcgattata ctcagtgttt tttagtccaa ccagcattaa cctatgcagg
      901 cgttgtcatt ggtggtacac gggtagttta cttaagtaat aacccagata aatctatttc
      961 tgtatttagc aaggaagaaa aaattcctta tctcatccag gcatgggtag atccgtttaa
     1021 taaagaggat aaaagcaaag caccgtttac cgttattcct ccggtatcac gattagaacc
     1081 cgcgcaagaa aaagtattac gcattattca taccaaaggt gtgtcattgc ctgatgatcg
     1141 cgagtcagtc ttctggctga atattaaaaa tattccgccc tctgcgagca acaaagcggc
     1201 gaactcactg gaaatcgcag ttaaaacgcg tatcaagctg ttctggcgac cggcaagcat
     1261 tcgcctgatt cccgaagatg ctgcaccgaa ggtgaagtgg caccgggaag ggcgaaacct
     1321 gattgcagag aatcccaacc cgattcatat cagtgtgatg gatgtcatcg ttgatggtca
     1381 tgatgtgccc ctcaacatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
     1441 ttctgcggga ggtcaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
     1501 gataaaaatg acgctataag caagggataa ggataggcgt attatgatga cgaccagaat
     1561 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaagcctga tggccgaggt
     1621 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
     1681 gtttaatcgg gcaaacctga tgccccctgg tgtttatagc gttgatattt ttatcaacgg
     1741 taaaatgttt gaacgtcagg atgtgacctt tgttcaggat aatcccgatg ctgatttaca
     1801 cgcctgcttt gtcgctatta aaaaaacgct aaccaccttt ggcgtaaaag ttgatgcgct
     1861 gaaatcgctc aatgatgtag atgaaacagt ctgtattgat cctgggccac gcattgaagg
     1921 ctcatcatgg cagttcgaca gcgataaact gcaactgaat atctccattc cccaaatcta
     1981 tatggatgcg atggcttatg attacatcag cccctcgcgt tgggatgagg ggattaacgc
     2041 gctcaccatc aactacgatt tttctggttc acatactcta cgttcggatt atggttcaca
     2101 agagacagat accagttatc tcaatctgcg caatggactg aatattgggc cgtggcgatt
     2161 acgcaattac agcaccttaa atacgaccga tggcagtgca gaatacaact ctataagcac
     2221 ctggatacaa cgtgatatag ctgcgttaag aagccagatt atgataggtg atacgtggac
     2281 ggcgagcgat atcttcgaca gtacgcaaat tcgtggcgca cgcttgtata ccgataacga
     2341 tatgttgcct gccagccaaa acggatttgc ccccgtagtg cgtgggattg caaagtctaa
     2401 cgccactgtc attattcgcc agaatggcta cgtgatttat cagtcagccg ttccacaagg
     2461 tgcttttgaa attaccgacc tcaacaccgc cagtacaggt ggtgatctgg acgtaaccat
     2521 caaagaagaa gacggtagcg aacaacgatt tactcaacct tatgcgtcgt tagccatcct
     2581 caaacgtgaa ggtcaaactg atgttgatgt cagtgtggga gaattacgcg atgaagatgg
     2641 ctttacccct gacgttcttc aggtgcagat ccttcatggt tttccctacg gtttcacctt
     2701 gtatggaggt atgcaggctg ctgaaaagta tggttctgcc gctttgggtg tcggtaaaga
     2761 gcttggcgca ctgggtgcaa tttctttcga tgtgacacat gcccgtgcga aatttagcca
     2821 tgctgatacg gaaaccggtc agtcttatcg ctttctgtat tcgaaacgct ttgacgatac
     2881 ggataccagc ttgcgcctgg ttggctatcg ttactccacc gaaggttact atactctcaa
     2941 tgaatgggcg tcgcggcgta ataatcctga agatttctgg gaaaccggta accgccgcag
     3001 ccgtgtggaa ggaacgttaa cgcaatcact gggcagagac tatggcaact tatatctgac
     3061 attaagtcgc cagcagtact ggcataccga tgatgttgaa cgattaatgc aatttggcta
     3121 cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca atactgcccg
     3181 gcaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct acatgctctc
     3241 tttatctgtt cctttatcgg gctggtgggg aaatagttac gccacctatt ctgtttcaca
     3301 aaacgataat tccggtagct cacatcaact tggactcagc ggtacggcgc tggaaagaaa
     3361 taacctttcc tggaatttaa tgcagtccta taacagccat gatgatgagg ttggcggtaa
     3421 tatgtccctg acctatgatg gcacttatgg cacggtgaac ggcagctata actacagcca
     3481 aaattctcag aggctgaatt atggtatcag agggggaatt ctggcgcaca gcgaaggcgt
     3541 aacgttaagc caggagttgg gtgaaaccat agctcttgtt aaagcaccag gggctgccgg
     3601 gttagaaata gacaatatgc gcggtgctgc gacggactgg cgcggctata cggtcaagac
     3661 acagctaaac ccttatgatg aaaaccgggt agcaatcagc gataactatt tctcgaagtc
     3721 gaatatagaa cttgataata ccgtcgttac gatggttccc acccgtggtg cagtggttaa
     3781 ggccgaattt gttactcgtg ttggttatcg tgtgcttttc agggttgcgg gtacaaaagg
     3841 taaaccagca ccttttggcg ctattgctac agtacaaaat acaagctccg ctgattcagg
     3901 aattgtcggt gaccaggggg agctttatct ctcgggcctt cctgaaaaag ggcaggtgat
     3961 gctctcctgg ggagaaaatg ccgccactac atgcaccttc gattattcac tttcaatacc
     4021 agaaagtgaa agcggcttaa ttgaacaagg tgtgacatgt cattaaaagg atttttatga
     4081 acaagtatat tataccatgg tgctttattg tttttatctt ctcctttagt ggcgcaacat
     4141 ttgcggcccc aaaaggtatc tgcacttcag ataatgggtc ctttcatagc acacttgatt
     4201 tttccggcta tctgattacg gcagacgaga acagagtggg aaagaccttt aataaaaccg
     4261 tgacaaatgg ggactcttat cctgctcact gccattgtga tacagggaac gtgggagagt
     4321 ttccttatat ttattataca gcgagaataa acgagtcctt aggttatgca ggtgttcgtt
     4381 ctaatgtaaa ctactttaat ttgaatccta atctggatgt tggaatatcg atagaccttc
     4441 tcggtgtagg atacattaat gcaccttttg aataccatgc taacaggcct actggaggtt
     4501 catataaatg tagtcgcacg gacccattaa gtatttccag tggtgcaaaa gcaataattt
     4561 atttttatat taagaagact tttgctggaa agttaattat tcctgaaact ctcgtggcga
     4621 aattgtacgg aaccataagc cgtgacactc cggttgatta ctcacaacct atggcggatg
     4681 tttatattcg tggcgatatc actgcaccgc aaagctgtga aattaacagt ttaagaccca
     4741 ttaattttga ttttaaagaa attcctgcgg cagatttttc ttcggtagtt ggaagtaccg
     4801 tgacaacgca taaaattacc aaaaccgtca ccattgagtg tgaaaattta ggaatactaa
     4861 atactgatga tatcagtacc tctttttatg ctaccgaacc cagtactgac aactcaatgg
     4921 tcgtgacatc aaacccgaac gttgggatta aaatttatga taaaaataat aaggaaatca
     4981 atgttaacgg tggtgaactg ccgacagata tgggtaaatc aaccgtcttt ggtgaaaaag
     5041 ccggtagcgt cactttttct gctgctcctg caagccttac gggggctcgc cccgcgccag
     5101 gaacatttac cgcgactgca acgataacaa ttgagattgt acgctaaacg aactgacagc
     5161 ggtgttcctc acaccgttgt catcttacgc a
//
LOCUS       DAZGTJ010000187         5171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000187 DAZGTJ010000000
VERSION     DAZGTJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.187"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..1409
                     /gene="alaC"
                     /locus_tag="ACHK8E_005012"
     CDS             171..1409
                     /gene="alaC"
                     /locus_tag="ACHK8E_005012"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP0347127.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1474..1545)
                     /gene="ypdK"
                     /locus_tag="ACHK8E_005013"
     CDS             complement(1474..1545)
                     /gene="ypdK"
                     /locus_tag="ACHK8E_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP0347128.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1901..2821)
                     /gene="lpxP"
                     /locus_tag="ACHK8E_005014"
     CDS             complement(1901..2821)
                     /gene="lpxP"
                     /locus_tag="ACHK8E_005014"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP0347129.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3174..3416
                     /gene="yfdY"
                     /locus_tag="ACHK8E_005015"
     CDS             3174..3416
                     /gene="yfdY"
                     /locus_tag="ACHK8E_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP0347130.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3493..3768)
                     /gene="ypdI"
                     /locus_tag="ACHK8E_005016"
     CDS             complement(3493..3768)
                     /gene="ypdI"
                     /locus_tag="ACHK8E_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP0347131.1"
                     /translation="MKVNLMIFSLFLLVSIMACNVFAFSISGGRSETSYKETEKTSVM
                     TTTHSTKLQPSQAILFKLREDAPPLNLTEDITPPFSTKANYLIHPVR"
     gene            4064..4696
                     /gene="yfdX"
                     /locus_tag="ACHK8E_005017"
     CDS             4064..4696
                     /gene="yfdX"
                     /locus_tag="ACHK8E_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP0347132.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         1408 a         1164 c         1245 g         1354 t
ORIGIN      
        1 tagcgcagat aaatttcctg tttttaaagc aaagttcctc atttaacgtc attaataaaa
       61 atgttctaaa acgctggaca gacagaaatt aatcaggcta tttcaaaaca gccaccatcg
      121 tggcagcgtc gctcggacgg tccgggcgct aacgttaatc tgaggaaatt atggctgaca
      181 ctcgccctga acgtcgcttt acgcgcattg atcgtctccc gccctatgtt tttaacatta
      241 ccgctgaact gaaaatggct gcgcgtcggc gcggcgaaga tattattgat ttcagtatgg
      301 gtaacccgga cggtgcgact ccgccgcata tcgtcgaaaa attatgtacg gtggcccagc
      361 gcccggacac gcatggttac tccacgtcac gcggcattcc gcggttacgt cgcgccattt
      421 cccgctggta tcaggatcgc tacggcattg agatcgaccc ggaatcagaa gccatcgtca
      481 ctattggttc gaaagaggga ctggcgcatc tgatgctggc gacgctggat catggtgaca
      541 ctgtactggt gcctaatcca agctacccga ttcatattta cggcgcggtg attgccgggg
      601 cgcaggtacg ctcagtgccg ctggtggaag gtgtcgattt cttcaacgaa ctggaacgcg
      661 ccattcgcga aagttatccg aaaccgaaga tgatgatcct cggcttcccg tcgaatccaa
      721 ccgcgcaatg cgtggagctg gagttcttcg aaaaggttgt ggcgctggcg aaacgctacg
      781 atgtgctggt ggtccatgac ctggcctatg ccgatatcgt ctacgatggc tggaaagcgc
      841 cgtcaatcat gcaggtgcca ggtgcacgcg atgtggcggt cgagttcttt acgctgtcga
      901 aaagctacaa catggcgggc tggcgtatcg gctttatggt tggcaacaaa acgctggtca
      961 gcgcgctggc acgtattaaa agctatcacg attacggcac ctttacgccg ttgcaggtgg
     1021 cagcgattgc ggcgctggag ggcgatcaac agtgcgtgcg cgacattgct gaacagtaca
     1081 aacgccgccg tgatgtactg gtaaaagggc tgcatgaagc gggctggatg gtcgaaatgc
     1141 cgaaggcttc gatgtatgtc tgggcgaaaa tcccggaacc gtatgctgcc atgggctcgc
     1201 tggaatttgc caagaagctg ctgaacgaag cgaaggtctg tgtctcgccg gggattggct
     1261 ttggcgacta cggcgacacg catgttcgct ttgcactgat tgaaaaccgc gaccgtattc
     1321 gtcaggcgat tcgtggaatt aaagcgatgt tccgtgccga cggtttacta cccgccagca
     1381 gcaaacatat tcacgaaaac gcggaataaa caccatcgcc agaaatgcaa acaggagcca
     1441 gatggctcct gtttttcatt gcacagtttg cttttagatc atcagggcaa aagtaccggc
     1501 ccaaacgatg accataaaag aaatgcccat aaagaaatat ttcactttac atctctccgc
     1561 gtaattactg ttacgcctga atgacttcat tacacctgat acagtgagtt gacaccgggc
     1621 aaaacgacct taaaagtcct gtattttacc gtgggcatca ccccagaatt ctgagctaaa
     1681 ctgctccaga cggcgctaat gtacgcctaa aactgaaacg agacaagagg cattttctta
     1741 tttactttta gtaacttaca ggtgtctcat atcggcgaca gtttaatttc tgacggtaat
     1801 aattagagat tcagcgaagg aaaaaacctt aagaaaaaag ccataaaaac catgaggtta
     1861 ttatggctga tttgaggagg gaaagagtaa gagcagtttg ttaaatgtat agcgacgatt
     1921 ctcccaccgg gcgcgtttta aagcgacggt ggatccagag gtactgctcc ggtgcgcgca
     1981 tgatctcttt ttcgataatc ttgttcatat aggcagcggc ttgattttca tctgccgggt
     2041 agccttccat ctctggggtg atgtacaaac gatatccgct gtaatctgct tttcttacca
     2101 tcgttacggt taacatggct gcgccagaga gacgtgaaag gacatacgta ccattggttg
     2161 tggcgacatt ttccaccgca aagaacggcg cgaaggagct gcctttacga ccataatcct
     2221 gatcgggagc aaaccatacc gcttcacctt tcttcagtgc accgacaatg ccgcgcagat
     2281 tatttctgcc gatcatcgct ttgttagagc gcatgcgccc acgggtctgc acccattcca
     2341 tcagctgatt attatgtgga cgataggtag ccatcattgg ttggcacagt cccatcacgc
     2401 ggccgcccag ttccagcgac ataaaatgga cgccgacaac cattacgccg cgattttgca
     2461 tttgtgcgcg tttaaggtta tccaaccctt caacatcaaa ccatttacgt acgcgactgt
     2521 cgggccagaa ccacgccatg ccggtttcta ccagcgccat gccgagtgaa cgaaagtttt
     2581 cggcaatcat cttctcgcgt tcttccgcag aatgctgtgg gaaacaaagt tccaggtttt
     2641 tacgggcgat agattcacgc cgtttcagga atggtcgcgc cattgcgcca atacgcgtgc
     2701 cgagaaagca gagaacagga taaggaagct gtacccaaag ccagagtaca ccaagcccaa
     2761 accatgtgag ccagtagcga ggatgtagaa actcgcggga aaatttgcat tgtggaaaca
     2821 tagttacctt ctgtagatac gaattgttgt atcgcagaag attccatgtc gacagcggta
     2881 cactcaattg gttgggccat catgtacgac aatggttcct gtaaagagtg taaagactag
     2941 tcaggacata accacgagga gcatgaatgc ggtggctcta ctttatcact ggctgactgc
     3001 tttcagctac tgattattaa tcgtgcaaag taaaaaattt tccgcacgca atagcgttgc
     3061 atttaacgtg ctgttttcat tgatgcttag caaaaattgc cagaggttaa ggattgtctt
     3121 actattaaag tgactgaagt tacaggtttt ttagcgcggc aggaggttga gctatgattt
     3181 atttatggat gtttctcgcc ctgtgtattg tctgcgtaag cggatacatc ggtcaggtac
     3241 tgaacgtggt ctcggcggtc tcttctttct tcggcatggt gatcctcgct gcactcattt
     3301 actacttcac catgtggtta actggcggta atgaactagt gacggggata tttatgtttc
     3361 ttgccccggc ttgtggcttg atgattcgct ttatggtggg gtatggcagg cgctagtggg
     3421 tcaggatggt tcatttgggc aggtgttcac atcagcatca ggcatgattg ttcacctgcc
     3481 aggtgacaga cgctatcgca caggatgaat aagataattc gcctttgtcg aaaaaggggg
     3541 cgtaatgtct tctgtgagat ttaatggtgg cgcatcttct ctcaacttaa acaaaatcgc
     3601 ctgtgatggc tgaagttttg tagaatgagt tgtcgtcatc actgacgttt tttctgtctc
     3661 tttatagctc gtctcacttc taccaccaga aatggaaaat gcaaaaacat tgcatgccat
     3721 aatagagacc aataaaaata agctaaaaat cattaagttt actttcatag aaagttatcc
     3781 tgaaaatggt tcactgagaa cctacgatgt tatggctgta tttacattta attaatcagt
     3841 atttacatcg atataataaa tgacatctct ttgtggtata taagaatagt tctctgtgac
     3901 aggaagcata ttcctacaat tgtaagacta aaatacttct tgcgataata actacaactg
     3961 taagataacc ctttcaaaat gaccgttgct ctctgatttc tcatttcatg ctcacccaat
     4021 atgatggcgg cgttttctaa aactgttaaa gaatgaggta agtatgaaac gtttaattat
     4081 ggctacgatg gtcacagcaa ttctggcatc ttcaactgta tgggctgctg ataatgcacc
     4141 ggtagcggca caacagcaaa ctcagcaagt tcagcaaacc cagaaaacgg cagcagcaga
     4201 gcgtatctct gaacaaggtt tatatgcgat gcgtgacgtg caggtagcgc gcctggctct
     4261 gtttcatggc gatccggaaa aggcaaaaga actgaccaat gaagcttcag ctttgttgtc
     4321 agatgacagt accgaatggg caaagttcgc taagccaggt aaaaagacca atctcaatga
     4381 tgaccaatat attgtcatta atgcatctgt cggaatttct gagagttatg tagcgacacc
     4441 cgaaaaagaa gccgcgataa aaattgccaa cgaaaaaatg gcgaaagggg ataaaaaggg
     4501 cgcaatggaa gagcttcgcc tggccggtgt tggtgtaatg gaaaaccaat atctgatgcc
     4561 gctcaaacag actcgtaatg cacttgctga tgcgcagaaa ctgcttgata aaaagcaata
     4621 ctacgaggca aacctggcgc ttaaaggtgc ggaagatgga atcatcgtcg atagcgaagc
     4681 actgtttgtg aactaaagct gacaggcaat atattgaggt aaaggatttt actccctcca
     4741 gccgacaatt ttttatggcc acaatacgac aggttgtggc cataaaatta tttggcgctc
     4801 gcggtgctat aaactcgcat tttatattct tcaaagtaaa ccgcaataat cccgtgtcat
     4861 cataaatcat ttattatgtt atgttttatg gtacgcttta aaaagcgtaa tcataccctt
     4921 acttgtacat caccggcgct tcttacagtt gtaagaataa catcacaaat gtcgcggccc
     4981 tctctgatta ccgcgaacca atatttatca tattcttctt ttcgtcctca ctacttacca
     5041 tgcagaaagg aattggaagt ggcgatgaaa tgattatcag cacttgatac aggaatttaa
     5101 aaatatcgag tgcagctacg acgatatttt gcggattatt tatagagagc attatgcctt
     5161 aaataaacgc t
//
LOCUS       DAZGTJ010000188         5144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000188 DAZGTJ010000000
VERSION     DAZGTJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.188"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1104
                     /locus_tag="ACHK8E_005018"
     CDS             <1..1104
                     /locus_tag="ACHK8E_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0347133.1"
                     /translation="RQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDS
                     AHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQ
                     QRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMADIISELNGLSDKIQKLSD
                     DATTANVDPADMAAQIALITSRINDLTASVILMHAPKGVAVASGEHLQLAAVKNLQIN
                     AGNNADIGVVKNMFIGVGRALSVFVRKAGIKLIANKGAVSVQAQHDLMELLAKKSIEI
                     VSTEDEIRISAKKKITINGGGSYIRIDSGGIEPGTPGDYNVKAVHYGRQPKASEKVPM
                     PEFPILSAVDSSDFCLECLLNAIKNDDAVVEGA"
     gene            1107..1928
                     /locus_tag="ACHK8E_005019"
     CDS             1107..1928
                     /locus_tag="ACHK8E_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFP0347134.1"
                     /translation="MTSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYMPRVLKQLAARPDMEWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTAANNSDETVEIITLDEPLWQKIADREDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQAVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            1972..2526
                     /locus_tag="ACHK8E_005020"
     CDS             1972..2526
                     /locus_tag="ACHK8E_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP0347135.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSVTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNKNLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            2567..4516
                     /locus_tag="ACHK8E_005021"
     CDS             2567..4516
                     /locus_tag="ACHK8E_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFP0347136.1"
                     /translation="MWGMLDTGGGIRAQLAEDNKVGNCGREWHVGFFFDGVGRNIDQD
                     ASEQRLSNVARLFRAYPDEDSNTDFVCHNKFYFSGMGTPYNEELISKLYTIMDQSLES
                     MKEDLNDFAKETHEEISKEIRNRRGSWNETLKKIQDKLLDPKELRKKRNDIFKNMAKK
                     SIVESTQWIRDNEIISAFFLTGEVTRINAAKVLFANAFVENSTNKNDSTAIKIKSISV
                     SLYGYDTGAALARKFLDELLEEFCEKEGEDKYLFKKVPVNIVFAGFFDCSRHSPASNN
                     NGLDYFLSLPGEITKNNKLKTAGKIAKVAFGEKAIELDTILPGTVKNALHLVAAYERR
                     LWRSLYQLGGMNAEHKEILLPGCSEDVGGGLKPDEQKPSAELCRVALQKMYEAAYDAG
                     VPYTDFSVLDENDSKVSRYFLMNDAVEGKSVKEWMKSYEMEVGQCQKETQSASESKVN
                     DTDNKNDLPFDFYLDIYFKWLANQYYLYCTELYQLDEKLSLAHRKQISGHGPLAGTRI
                     NPNPEADEINAQIAELKSHWGWLDDVRRVATGLSNDFNYGRPMDTRMLNHEDIFRPAW
                     KRAELFLDYYHKAWNGEELTEISWLGIDTIHSYFTHDLQTVDTGASINESFFLRRMAE
                     YPKAKEKPEEKSEEQSPSPDLSGDD"
     gene            4525..5061
                     /locus_tag="ACHK8E_005022"
     CDS             4525..5061
                     /locus_tag="ACHK8E_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347137.1"
                     /translation="MSLYNKINLNDESEVFMILKIISRLFFFIIVVVYFLVFILSLDG
                     GGRLSILNILAAIGGNTFWCVLVVISLAGSAGICVYMLKKNCLTNSAVTILSTSFIFT
                     TSIFVILFLKAEFVLDWLYCIYIEIFFNGDFYSGQERLLQTVPFMIRTYFIFLMANLF
                     LFWLSFFFLIKNKTLHKA"
BASE COUNT         1514 a          979 c         1327 g         1324 t
ORIGIN      
        1 cgccagtcgc gcccgtatgc gggagacacc tacggcctgc acctgccgct gctggcagga
       61 acggaagtca gtatcgcctt tgaggaaggc aacccggacc gaccttatat tgcaggcgta
      121 aaacacgact cggcgcacac cgaccatgtg accattcaga actacaagcg caacgtactg
      181 cgtaccccgg cgaacaacaa aatccgcctc gacgatgagc gcgggaaaga gcatatcaag
      241 gtcagcaccg aatatggcgg caagagccag ctaaacctcg gtcatctggt ggatgccggg
      301 aaacagcagc gcggggaagg ctttgaactg cgtaccgata tgtggggcgc ggtgcgggcg
      361 aagaaaggga tttttatcag cgccgatgcg caggacaagg cgcagggtca ggtcagagag
      421 atggccgaca ttatcagcga actgaacggt ctgagcgata aaatccagaa actgtccgat
      481 gacgcgacga cggcgaacgt cgacccggcg gatatggccg cgcagatagc attgataacc
      541 agccggataa acgacctgac cgcctcggtg attctgatgc acgcgccgaa aggtgtggcg
      601 gtcgccagcg gcgagcacct gcaactggcc gcagtgaaaa acctccagat aaatgcggga
      661 aacaacgccg atatcggcgt ggtgaagaat atgtttatcg gcgtggggcg ggcgctgagc
      721 gtgtttgtgc gcaaggcggg tatcaagctg attgccaata aaggcgcagt atcggtacag
      781 gcgcagcacg atttgatgga gttactggcg aagaagtcga tagagatcgt cagtaccgaa
      841 gatgagatca ggatttcggc gaagaaaaaa atcacgatta atggcggcgg gagttatata
      901 cggattgata gcggcggcat tgagccggga acgccggggg attataacgt caaagcggtg
      961 cattacgggc gccagccgaa agcctcggag aaggttccta tgccagaatt tcctatcctt
     1021 tcagccgtgg actcatccga tttttgtctt gaatgcctgc tgaatgccat caaaaacgat
     1081 gatgctgttg ttgaaggagc gtaatcatga cttcagaaca acaactggca gaccactggc
     1141 ttaagcgaaa cgaaacgtta aacgccttta ttttatttga aggtggagcg ctctctcagg
     1201 aaatgcttca gatcatgatg gagcattata aataccgacg aatttccctg tatcaactgc
     1261 ccgatcttaa aaaggtagct gcttacggtc cctggttggt tcgtattgaa agtgaaaagg
     1321 atctggcgat ggtgcttgtc gggaaactac ctgtcgcagg cattactttc tctgaattgc
     1381 agctggagcc actggcgtcc cggcttgctg tgggatgtat ggcaaaaacg ccggatggtc
     1441 gtgaagttct gctgaggtac tacatgcccc gagtgctgaa acaactggca gcccggcctg
     1501 atatggagtg gcatccggtg atgttctctc agatagaatc atggtggatg ctggaagata
     1561 cgcggtggca aaggctgaac atgccgctta ctgcggcaaa taacagcgat gagacagtgg
     1621 aaattataac gcttgatgaa ccgctctggc agaaaatagc ggatcgggaa gatgtatctg
     1681 cgcttttaag agagtggcgc accatgaaaa cgagtgagca cttcccgccc tgtattcagc
     1741 gaaatatggt ggagaaggca ttgaataagg ctcaggcggt cggattgaca aagccactgg
     1801 atcagaaaat ttatgcgttg agctatctga atggaaagaa agcgtatctt gagtcagccg
     1861 aatttcaggt catgctggaa aaggtgaaac gaaatgaaat atcgctgtct gaccttggtc
     1921 gccagtgaca tacatctttg ccaaccaatt gataaaaggg aataaaatct gatggggcta
     1981 tttacccgac tggaaaaatt tgaccaaaag cttacgcgtg gttatgcccg ctgggggcgt
     2041 tgggtgtggc ggatgctgat cgccgtgcca gtactggtcg tattgtggaa tatagggcag
     2101 gcagtatggg gaggtccaag aggtggagtg attctggaaa tccacagtga aattgaccgt
     2161 ccgatactgg gattcagtgt taacggcgtg gcgggcgcga atgcttttgc aaatggtggc
     2221 ggttcagtca cctgttgcgg tgatgtcagt ggggatacgg cagaggttat ctggacatta
     2281 agtactactc gtacacagta taatgctgga atgcgtctgg agaaacgtaa caagaatttg
     2341 cctctcccaa aaagagaatg gggagaaaat tatctgcacg tgcattttat gcctggcgat
     2401 aaagtgttgc tggggtggag taaagattct ttttctccgt atgaagatct tcacaacggg
     2461 gggtacaaga cccgcgttcg gcaggatgtg aaagataaac tttatggcac agggaaaatg
     2521 aattgataga tttctgtaaa aatcacgttc agcaggaggc gtaataatgt ggggaatgtt
     2581 agataccggt ggtggaattc gggcacaact cgcggaagac aacaaagtag gaaactgtgg
     2641 gcgggagtgg cacgttggtt ttttctttga tggcgtgggg cgcaatattg atcaggatgc
     2701 ttcagaacaa cgcttaagta atgtggctcg tttatttcga gcttatcctg atgaagattc
     2761 caatactgat tttgtttgtc acaataaatt ttatttttcc ggtatgggaa caccctataa
     2821 cgaagaattg atctcaaaat tatatacaat catggatcag tctctggaaa gtatgaagga
     2881 ggatttaaac gatttcgcaa aagagacaca tgaagaaatc agcaaggaaa tccgtaacag
     2941 aaggggatcg tggaatgaaa ccttaaagaa aatccaggat aaacttctgg accctaaaga
     3001 acttagaaaa aaaagaaatg atatctttaa aaacatggct aaaaaatcca ttgttgagtc
     3061 aacgcaatgg atccgcgaca atgaaattat tagtgctttc ttcttaacgg gggaggtcac
     3121 gagaattaat gcagcaaagg tgttgtttgc gaatgctttt gttgaaaaca gcacgaataa
     3181 aaatgattca actgcgatta aaataaaatc aatatctgtt tcgttgtatg gctatgacac
     3241 cggagctgca ctggcacgaa aatttctgga tgagttactc gaagagtttt gtgaaaaaga
     3301 aggtgaggat aaatacctgt ttaaaaaggt accagtaaat atcgtctttg ccggcttctt
     3361 tgactgttca cgtcattcac cagccagtaa taataatggg ctggactatt ttttaagtct
     3421 tccgggggaa attaccaaga ataataagct gaaaacagct ggcaaaatcg ccaaagtcgc
     3481 atttggcgag aaggccatcg aactggatac catcttaccc ggtacagtta agaacgcgtt
     3541 acatctggta gcggcttatg agcgacgtct atggcgcagt ctttaccagt tggggggtat
     3601 gaatgcggaa cataaggaaa ttttactgcc tggttgtagc gaggatgtgg gcggtggtct
     3661 gaagccagat gaacagaaac caagcgcaga gttatgtcgt gtggccctgc aaaagatgta
     3721 tgaagcggca tatgatgctg gggtacctta tactgatttc tctgttctgg atgagaacga
     3781 tagtaaagtg tctcgctatt tcctcatgaa tgatgctgta gaaggcaaat ccgttaaaga
     3841 gtggatgaaa agctatgaaa tggaagtggg acagtgccaa aaagaaacgc aaagcgcctc
     3901 tgaaagtaaa gtaaatgata cggataataa aaatgacctg ccctttgatt tttatcttga
     3961 tatatatttc aaatggttgg caaatcaata ctatctttac tgtactgaac tttatcaatt
     4021 ggatgaaaaa ctatcacttg cacatcgcaa acaaataagc gggcatggcc ctcttgccgg
     4081 aacgagaatt aaccctaatc ctgaagctga tgaaatcaat gcacagatcg cagaacttaa
     4141 atctcactgg ggatggctgg atgatgtaag gcgtgtcgct acaggattat cgaatgattt
     4201 taattacgga aggccaatgg atacccgcat gctaaatcat gaagatattt ttcgcccggc
     4261 atggaagcga gctgaactgt ttcttgacta ttatcataaa gcatggaacg gagaagaact
     4321 cacggagata tcatggctag gcatagatac tattcattct tattttactc atgatttaca
     4381 aactgttgat accggggctt ctattaacga aagttttttc cttcgtcgta tggctgaata
     4441 tcccaaagca aaagaaaaac cagaagaaaa gtcagaagaa cagtcaccat ctccagattt
     4501 gtcaggtgat gattaaatca ggtgatgtct ctctacaata aaataaattt aaatgatgaa
     4561 tctgaggtgt ttatgatact taaaataatt tcaaggttat tcttttttat tatagtagta
     4621 gtttattttt tagtatttat actttctctt gatggcggtg gacgacttag tattcttaat
     4681 atattagccg ccattggtgg taacacgttc tggtgtgtgc tggtggtgat atccctggca
     4741 gggagcgcag gaatatgcgt ttatatgctc aagaaaaact gtctgactaa ttctgcggtg
     4801 acaatattgt ctacttcatt tatctttaca acaagtatat ttgtcatcct ttttcttaag
     4861 gcagagtttg tactggactg gttgtattgc atatatattg agatattttt taatggggat
     4921 ttttattcag gtcaagaaag attattacag acagttccgt ttatgattag aacatatttc
     4981 atttttctaa tggctaacct ttttttattc tggctatcct tcttttttct tattaaaaat
     5041 aaaacattgc acaaagcgta gtatcaaatg aggtaagtat atgaagtact aaccaataaa
     5101 ttaaggaaaa taaaatggtt actcctactc tgaatttcga ccac
//
LOCUS       DAZGTJ010000189         5137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000189 DAZGTJ010000000
VERSION     DAZGTJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.189"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            567..2549
                     /gene="chuA"
                     /locus_tag="ACHK8E_005023"
                     /gene_synonym="shuA"
     CDS             567..2549
                     /gene="chuA"
                     /locus_tag="ACHK8E_005023"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP0347138.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            2598..3626
                     /gene="chuS"
                     /locus_tag="ACHK8E_005024"
     CDS             2598..3626
                     /gene="chuS"
                     /locus_tag="ACHK8E_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP0347139.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(3687..4217)
                     /gene="dctR"
                     /locus_tag="ACHK8E_005025"
     CDS             complement(3687..4217)
                     /gene="dctR"
                     /locus_tag="ACHK8E_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0347140.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4370..4936)
                     /gene="slp"
                     /locus_tag="ACHK8E_005026"
     CDS             complement(4370..4936)
                     /gene="slp"
                     /locus_tag="ACHK8E_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP0347141.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1379 a         1140 c         1246 g         1372 t
ORIGIN      
        1 tcaagatgct tcgtcagcta acgttcttgt taccgactaa atttcagcgc gggaaaaagc
       61 tgagaaccca gagatatcgc ggcttgcaga gcaaagagaa gagaaaatta tcactgttaa
      121 cgacaaagtt tagtgatgag gtcatggggg aataaccata aaaacaaagt ggtttccagg
      181 gcgcaggata agtttcataa ccccctgttt attgtgttaa tggcggttaa aaaaaagctt
      241 gatcataaaa aattgatcga taataattct cattatcacg tttgcattta acaaaatacg
      301 gccatatgga taacaaagcc atatcattta caaggggtaa cgcacagcgc tcttcactcc
      361 tgatggggca tatcggtttg cctgacaaag agggcgtgct tttgcgtatt tgccgagata
      421 ccaggcaatt tcatgggata taaacgcctg taaactcacc cggcgaatat tatcgctctg
      481 aattttaaat aaatttattt ttcgccagcc accccggtat taacccaggc agccagcatt
      541 ttttattttc ctcatggaga atcgctatgt cacgtccgca atttacctcg ttgcgtttga
      601 gtttgttggc tttggctgtt tctgccacct tgccaacgtt tgcttttgct actgaaacca
      661 tgaccgttac ggcaacgggg aatgcccgta gttccttcga agcgcctatg atggtcagcg
      721 tcatcgacac ttccgctcct gaaaatcaaa cggctacttc agccaccgat ctgctgcgtc
      781 atgttcctgg aattactctt gatggtaccg gacgaaccaa cggtcaggat gtaaatatgc
      841 gtggctatga tcatcgcggc gtgctggttc ttgtcgatgg tgttcgccag ggaacggata
      901 ccggacacct gaatggcact tttctcgatc cggcgctgat caagcgtgtt gagattgttc
      961 gcggaccttc agcattactg tatggcagtg gcgcgctggg tggagtgatc tcctacgata
     1021 cggtcgatgc aaaagattta ttgcaggaag gacaaagcag tggttttcgt gtctttggta
     1081 ctggcggcac gggggaccat agcctgggat taggcgcgag cgcgtttggg cgaactgaaa
     1141 atctggatgg tattgtggcc tggtccagtc gcgatcgggg tgatttacgc cagagcaatg
     1201 gtgaaaccgc gccgaatgac gaatccatta ataacatgct ggcgaaaggg acctggcaaa
     1261 ttgattcagc tcagtctctg agcggtttag tgcgttacta caacaacgac gcgcgtgaac
     1321 caaaaaatcc gcagaccgtt gaagcttctg atagcagcaa cccgatggtc gatcgttcaa
     1381 caattcaacg cgatgcgcag ctttcttata aactcgcccc gcagggtaac gactggttaa
     1441 atgcagatgc aaaaatttac tggtcggaag tccgtattaa tgcgcaaaac acggggagtt
     1501 ccggcgagta tcgtgaacag ataacaaaag gagcaaggct ggagaaccgt tccactctat
     1561 ttgccgacag tttcgcttct cacttactga catatggcgg tgagtattat cgtcaggaac
     1621 aacatccggg tggcgcgacg acgggcttcc cgcaagcaaa aatcgatttt agctctggct
     1681 ggctacaaga tgagatcacc ttacgcgatc tgccgattac cctgcttggc ggaacccgct
     1741 atgacagtta tcgcggtagc agcgacggct acaaagatgt tgatgccgac aaatggtcat
     1801 ctcgcgcggg gatgactatc aacccgacta actggctgat gttatttggc tcatatgctc
     1861 aggcattccg cgccccgacg atgggcgaaa tgtataacga ttctaagcac ttctcgattg
     1921 gtcgcttcta taccaactat tgggtgccaa acccgaactt acgtccggaa actaacgaaa
     1981 ctcaggagta cggttttggg ctgcgttttg atgacctgat gttgtccaat gatgctctgg
     2041 aatttaaagc cagctacttt gataccaaag cgaaggatta catctccacg accgtcgatt
     2101 tcgcggcggc gacaactatg tcgtataacg tcccgaacgc caaaatctgg ggttgggatg
     2161 tgatgacgaa atataccact gatctgttta gccttgatgt ggcctataac cgtacccgcg
     2221 gcaaagacac cgataccggc gaatatatct ccagtattaa cccggatacc gttaccagca
     2281 ccctgaatat tccgatcgct cacagtggct tctctgttgg gtgggttggt acgtttgccg
     2341 atcgctcaac acatatcagc agcagttaca gcaaacaacc aggctatggc gtgaatgatt
     2401 tctacgtcag ttatcaagga caacaggcgc tcaaaggtat gaccactact ttggtgttgg
     2461 gtaacgcttt cgacaaagag tactggtcgc cacaaggcat cccacaggat ggtcgtaatg
     2521 gaaaaatttt cgtgagttat caatggtaat catctgcccc gatatttcgg ggcatttatc
     2581 tggaaggaag agagacaatg aaccactaca cacgctggct tgagttaaaa gaacaaaatc
     2641 ccggaaagta cgcgcgtgac atcgcaggtt taatgaatat tagcgaagca gaactggcat
     2701 ttgcgcgcgt cacgcacgat gcgtggcgga tgcgcggcga tatccgtgaa attctggcgg
     2761 cgctcgaaag tgttggcgag accaaatgta tttgtcgtaa tgaatatgca gtccatgagc
     2821 aagttggtgc gttcacaaac cagcatttga acggacatgc cggattgatc ctcaatccac
     2881 gcgcgctgga tttgcgtctg tttctcaatc aatgggccag tgtttttcac attaaagaaa
     2941 atacgactcg tggcgaacgc cagagtattc agttctttga tcatcagggc gatgcattac
     3001 taaaagttta tgccaccgac aataccgata tggcggcatg gagtgagctt ctggcacggt
     3061 ttatcgccga tgagaatatg ccgcttgagt taaaagccgt tgatgcgcca gttgttcaaa
     3121 cgcgagccga tgccagtgtg gtcgagcaag agtggcgggc gatgaccgac gttcatcagt
     3181 tttttacgtt gctcaagcgc cacaacctga cgcgccaaca ggcgttcaat ctggtggcag
     3241 atgatttggc ctgcaaagta tccaacagtg cgttggcgca aattcttgaa tctgcacagc
     3301 aggatggcaa tgaaatcatg gtgtttgttg gcaaccgtgg ctgcgtacag attttcactg
     3361 gtgtggtaga aaaagtggtg ccaatgaaag gttggctgaa tattttcaac ccgacgttta
     3421 ctcttcatct attagaagag agcattgctg aagcctgggt tacccgtaaa ccgaccagcg
     3481 atggctacgt aaccagtctg gaattgtttg cccatgatgg tacgcagata gcgcaacttt
     3541 atggtcaacg tacagaaggc gaacaggagc aagcgcaatg gcgtaagcaa attgcttcgc
     3601 tgataccgga agtcgttact gcataacgta taattaacgg gaggtctggc tatttattgc
     3661 tgggcctctt tatattgatc aatcgttcac accagataat caatatgctg atggcgtacc
     3721 aactcattga tccgcttaag attgaggatc atcatgatat tgtgtttatg gcagtagaac
     3781 gtttttagcg atattttcag tttctccaga atatcttccg ttgtcatttg attcgcaatg
     3841 cagctcagaa cctgatgctg cttgcgcgaa agggacacat ccgcttcagg cacagagcag
     3901 taactgccat tgatgagtgc gacaaagtcg atgatggtga tagttcgcgg aacaaaggtc
     3961 accggtactt tccccaggca gcgtttaata ttaaagggag agaaaataat cacatggaca
     4021 ggttttaatc gttcaatctg agtgagcaaa ttagaatgaa atacattatt cattaatgta
     4081 tcaataatga cgaaggcatg ctgatgcaac attacgtcga tctcttcttc gttctgtata
     4141 tgaacgacat tacctttact cagaatgttt ttcatcgcag tgaagaacat cgtatccctg
     4201 gtaattataa gaaacataac cgactcctgg tgcggacatt aactttataa tcagtttgcc
     4261 aaaggtaatt aacaggacag ggcttataat aatagttagc tggctgtgta ttaatttgat
     4321 gtaatgcatg atgcagatgc tgagaaagca tctgcatctc tcggtggtgt tatttgacca
     4381 gctcaggtgt tacttgactc accgcattgg tgtagtaagg cgcgccccag cccggttccg
     4441 gccagaatgc accatagccg taatcccaca ggttataggt ggtattaacc acttctctca
     4501 aatgccacac ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga
     4561 taaagccagg ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt
     4621 tcactggatc aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa
     4681 tatcaggctt cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt
     4741 tgccattgat aacgttgata accttcccgc caaagcgcgc ttgttgacca acatataacc
     4801 ccggctggtt atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga
     4861 tattttgcgg aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac
     4921 cttttgtcat gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt
     4981 ctttatagtt ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga
     5041 tgtttatatc ccttctgttt agtttaattc tgttaatttt ggggcgtgac atgagtatcc
     5101 atcgccccat aaaatagcct ttttttaaag gtacata
//
LOCUS       DAZGTJ010000190         5134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000190 DAZGTJ010000000
VERSION     DAZGTJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.190"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            226..1071
                     /gene="mscS"
                     /locus_tag="ACHK8E_005027"
     CDS             226..1071
                     /gene="mscS"
                     /locus_tag="ACHK8E_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0347142.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1197..2417
                     /locus_tag="ACHK8E_005028"
     CDS             1197..2417
                     /locus_tag="ACHK8E_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFP0347143.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            2647..4392
                     /locus_tag="ACHK8E_005029"
     CDS             2647..4392
                     /locus_tag="ACHK8E_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0347144.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(4574..>5134)
                     /locus_tag="ACHK8E_005030"
     CDS             complement(4574..>5134)
                     /locus_tag="ACHK8E_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0347145.1"
                     /translation="IDAINKIKIELHRSSPFYAEPVDCVIWIKNNLIEANDYNPNVMA
                     PSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVT
                     CIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDE
                     VLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT         1296 a          994 c         1336 g         1508 t
ORIGIN      
        1 gataccggca acaatatggg ccgtgtatgc gctttcgcag tataagacgg aacagaaaat
       61 ccgcgaagcc acggggaaat gatgactgca gaagagcagg ttgtcatacc ggtgtatatg
      121 atgcgggata aaggcccgga gattcctgga aataaaatgt tccggggaat ataaaacagg
      181 gaaaaaccgg atgtgatgga cgcttctggc gaggcggttt catgaattgt ccgtaatcag
      241 gatatgttgc ccggttacgc cgcgaatctt gtggcagcag tggttattat tatcgggggt
      301 atcgctgcac ggatgatttc tggtgtgctg aataatctga tgctgggcag gaatattgat
      361 gccacagtgg cgctgttcct ttccgcgctg atacgtcatg ccattatagc ctttacgctg
      421 attgctgccc tgggacgtgt gggggttcag acagcttccg taattgcagt cctgggggct
      481 gcaggtcttg ctgttggact tgccctgcag gggtcattgt caaaccttgc agctggtatt
      541 cttctcgtga tgttccgtcc tttcagggct ggtgaatatg ctgacctggg tggtgttgcc
      601 gggactgtgc agaatgtaca tatattctca accacgatgc gtacactgga tggtaaaatt
      661 gttgtcattc cgaacgggaa gattattgcc ggagagattg ttaatttctc cagggagcct
      721 gaacgacgaa atgagtttat catcagtgtt tcatatgatg cagatattga ctgtgtgaaa
      781 caagtcctga cagatattgt gatgtcagaa gagagggttc tgaagaacag ggaaatcact
      841 gttcgcctga atgagcctgg ggagtcatcc atgaattttg tagtacgggt atggagcagg
      901 cgtgatgacc ttcagtctgt atactgggat gtactggaga gaattaagcg ggagtttgat
      961 tcagaaggga taagttttcc atatccacaa atggatatcc atcttgttcg ctccgggaag
     1021 caggcagagt cggtaaccca taacagtcgc tcagtcagtt ccagacagta aaatgaaaag
     1081 cgcattttat ttgtgaaaga tgaacagcct gaatctgtca ggctgttttc attgtgtttt
     1141 tatggtcatt tttggtatat gtactgtata taatgtcatc tgtacaagga tataaaatgc
     1201 caaagaaaat aatgacaggg attgatgttt ttgacgctgc catggagcgt attgaatggg
     1261 tgtttgatac gttttcatca gtttgtcttt ctttttccgg cggaaaagac tccactgtcc
     1321 tgtttcacct gatggcgaat attgcccgcc gaaaaaaacg ccgtttttct gtactgtttg
     1381 ttgactggga ggctcagtat cagtgtacca ttgagcatgt caccaggttg agggaaatgt
     1441 acgatgatat cacagatact tttttctggg tggcgctgcc tctgacgacc gttaatggag
     1501 tgtctcaggt acagcctgag tgggtatgtt gggaggacgg ggctgaatgg gttcgccagc
     1561 caccagaaga tgccataacc agagcgtctt tttttccttt ttatcagaat gcgatgacat
     1621 ttgaagagtt tattccggca tttaatcatt ggtttgcccg ggaaaaaggg atggttgctg
     1681 cgattcttac tggtgttcgc gctgatgagt cactgaatcg ttttatggga ctggtatccg
     1741 gacgtaagct ccgttatgcg gaggataaac cctggaccac ggcatcggct gagggatttt
     1801 actacaccat gtatcctttg tacgactgga aaacaagaga tgtatggatt taccatgcga
     1861 gaacacgtgc tgcctataac tctctttatg acctgatgta cagggctgga gttcctcttc
     1921 gcaatatgcg ggtgtgtgag ccttttggac cggagcaacg taaggggttg tggctttatc
     1981 atgtactgga gccggataca tggggcagaa tgtgtgctcg cgtatcaggt gctgtagggg
     2041 gagcgctcta tgcaaatgaa agtggtgctt attttgcttt gaggaaacag ataacaaaac
     2101 cagttcacca tacatggcag agttatgcaa tgtttctgct ggagtcaatg cctgagaaaa
     2161 cggcggagca ttacaagaat aagattgctg tttatcttcg gtggtggcag tctcatggat
     2221 ggccggatgg catccctgat gagcaggata atgaccaggg atgtaaagat attccctcct
     2281 ggcgtcgcat ctgtaagacg ttactgaaaa atgatttctg gtgtcggatg ctggcgttta
     2341 gtccgaataa gccccgacat tatgagcgct acctggccag aatgcgtgaa aaaaggaaag
     2401 agtggggaat attgtaaatt atatttaaac atcagaaata tgacggttaa tccaggatag
     2461 tagctaaaaa aataagattc cttgctgatt tatccctgtt tgatatgtaa aatctgaggg
     2521 gcttataagc aacggatata cgagtgctac aatatatgat aagttatata tattaaatgt
     2581 tcatcatctg tcaggggctg ttttatggct aaactgataa atatatcagg tgttaccgag
     2641 gagcggatga ctgccgggga aaagcgggtt gcatcccgtc ttgaggcatt tatgagtaat
     2701 gagtgtctgg tctggtacga tattccggtg ggacggaaaa accgacatcc tgattttgtc
     2761 attattgacc cggagaatgg tctggtattt cttgaagtca aggactggac cgttcacacg
     2821 ttgcatcagg taaatcacga acaggtcatt cttgaaacta acggtattct taaatcagaa
     2881 ataaatccgc tgctacaggt tcgtcgttat gcctgtgata ccgtaaatgc tttacctgca
     2941 gactcacgtt tgcgacagaa tgatggtcag tataaggggc gtctgaatct ggcctgggcg
     3001 tatggtgttg tgtttacccg tattacccgc cagcaactga aggctctggc tgggaatgat
     3061 gaaaatgctg tggaaaggat tttcccttca gcgcagacaa tctgtcagga tgatatgact
     3121 cagtctgttt tacctgatat attccgccaa aaaattgccg ggatgtttac gatgggattc
     3181 agaacccggg tgacaccaca ggttagagat attctgcgtg ctcatctgtt tcctgaaatg
     3241 accgtcagac agcactcaca aatcaaaata atggatattc agcaggagat tctggccaga
     3301 aatattggtg atgggcatcg tgttattcat ggggtagccg gctctggaaa aacaatgatt
     3361 cttttgttcc gttgtcttta cttggctgaa actacccctg gtaagattct ggtgctatgt
     3421 tacaacatta ctctggccag ctacctgcgg gaatgtattg agtccagggg actgaagtca
     3481 cgggttacgg tattccattt tcatagttgg tgcgcctcaa tgataaagcg ccatggaatt
     3541 caggtgactg ctggaggaaa ggattacccg gagaaatgct tcagtgcgct ggaagatgcc
     3601 gtgaacagcg gaacaattac tgatactgga tacgatgccg tacttgtgga tgaggggcat
     3661 gattttgaga gtcgctggct ggctctgata gcccgcctgt ttgataacgc gtcccgttca
     3721 ctgctcctga tgtacgatga cgcacagtcc atttacagac gggaacgggc tctgaacttt
     3781 tcgcttgcca gtgtggggat tcaggcgcag ggacgtactt ccgtcctgcc ggtaaattac
     3841 cgtaatacga aacgcattct gcactttgct tatgcattct cccgtgagta ttttgagaag
     3901 catcagaacc gtgaaatacc gtttgttcag ccacaggcgg gcggtgaaga gggcacagag
     3961 ccggaaattc tgcgttgtgc ttctgagagt gatgaggcag tgcaggttgt gggctggctg
     4021 gagaaacgct atacactgtg cggtcactgg agtgatatgg ctgttttgtg tcctgcagag
     4081 ttttcagtga aacatctgaa agaggtcatg acacaacgcg ggatacctta tgccacctgc
     4141 tttgacagtg aaggtaagaa gaaatattcg cgacgcaagg atgttgtgca tttactgacc
     4201 taccagagca gtaagggact ggagtttccg tatgttgcgg ttatcaatgc ttctttcgtt
     4261 cactcaggtg ctgcggatga gtctgaagtt attccggcgc tttatgttgc gttcacccgg
     4321 gctacacggg aattactggt gacctgttat cgtgaaaaca gtatcagtcg tcatcttgag
     4381 gattttgtat aagttgtgtt ttattattgc tgatatgtaa tcattcgttt tttgataatc
     4441 taagatgggt atttagcgag tattcagagt tgaattaata tcccccatgt ttttgatctg
     4501 tcaatagctg tccattacat ggggtgatca tatatcagtc ggtaattact ggataaagtc
     4561 accccctaaa tgattacttt attgtccatg ccggactaaa gttttcctcc tcaaatagtt
     4621 ctgttaaccc acttatttgt tttaatctca gtacctcatc tgtatccatg cccagttcac
     4681 tacctattcg gagatcatcc catcccattc gggaaagatc tcggacgata tcggagagcg
     4741 cgtctatctg gtgctttcct ctggccctgt tatgcctgat ggtcgaggcg atagagtaag
     4801 ctttaaagtt attatcatgc ttaatgcatg tgacaggtaa atatcctttt agttttttct
     4861 ctagttttgt tgtgcttttc ccaagcattt gtctgtgaaa tccgtcaata acggtgtatt
     4921 tccccttttg agctaaggtg acaacaggtt gagtgaatcc atcaaccaac agggagtgtg
     4981 ttaataacct tttttcggag ggagccatga cgttaggatt atagtcgttt gcttcaatta
     5041 gattgttttt tatccatata acacaatcaa caggttctgc gtaaaaagga ctactcctat
     5101 gtaactcaat ctttatttta ttaattgcat caat
//
LOCUS       DAZGTJ010000191         5122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000191 DAZGTJ010000000
VERSION     DAZGTJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.191"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            104..2251
                     /locus_tag="ACHK8E_005031"
     CDS             104..2251
                     /locus_tag="ACHK8E_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP0347146.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGVLIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            2311..3558
                     /locus_tag="ACHK8E_005032"
     CDS             2311..3558
                     /locus_tag="ACHK8E_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP0347147.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            3844..>5122
                     /locus_tag="ACHK8E_005033"
     CDS             3844..>5122
                     /locus_tag="ACHK8E_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFP0347148.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGGSKHRHENHEA
                     DTTAPSAPELTAGDNGSVSVELPGDANKGDTVEITFEDENGDEQKVTLEKGDNGWTSS
                     NPALIPDSQGDTATIAPDTVKDNSEVTAVAKDPAGNESTPVTVTSKTDGVADAPVLTI
                     PEVADSVANAAELKDGLQAEVKLPAGTVEGAVITLTVTHPDQSTENVTHNVTGDEVTA
                     GKVSMDIPEDAVVDGQNSVSVSLTQGSNPAKAGNKVEFVVDGQVPGDTNGDGVADVTP
                     AVAIPEATDGVNAKELKDGVQAEVTVPAGSAEGD"
BASE COUNT         1305 a         1051 c         1521 g         1245 t
ORIGIN      
        1 atgctggatt atctgtattc ccagggaatg ctcctgaaat ggagcggagt gaatctttct
       61 ggtgcagaag aaaaagtaac agggcagaag gacagataaa taagtgaaga gtatttttga
      121 aggattatca caggtaacgg cattactggg tacacctgta tctgctgaga cgctggctgc
      181 cggtacagtc cggacagatg tctccgggat tgattttcgt tctgttggcg agtttcttcg
      241 cagtgaaggg tttgataatc atctttcccg gcgggctccg gaagatattc cttcacttgc
      301 ggttcctgtg cttcttttac tgaatgcaca ggaagccatt gtggtggtgc gtattgaggg
      361 tgccggcctg gcccggcagt atcatgtact ccagcccggg ggactccgta agacactttc
      421 tcacgatgaa ttaatggctg tttattcagg ttactgctgg tttgttcagc cacaggcaac
      481 acaggatcgc cgttcggaac tgccggagta tgacatgacc ggggcatggt tctggcgggt
      541 actgtggcgg ttcaggccat attacggaca ggtcattctt gcgagtttta tcattaactt
      601 tcttgccctg gtcagttccc tgtatgtcat gaacgtttat gaccgggtca tacccaatcg
      661 ctcctgggaa acgttatgga tgctgagtat cggggtgatt gcagccattc tgtttgagtg
      721 tgctgcaaag ttaatccgga gctatctgac ggatattgcg ggtaagaagg cagacctgat
      781 tatcagctca gcactgttaa ggcgggtgat ggcagtgaaa atgtcggaca ggccggcgtc
      841 ttccggttca tacgccagca atttgcggga atatgagtct gtcagggagt ttatgaccag
      901 cgccacgctg ctggcttttg tggatttgcc gtttatcctg cttttcatcg gtgtcatcgg
      961 gcttgtgggg ggctggcttg ctgttgttcc tcttacactg gttccgctgg tgattctggc
     1021 aggaatatta ctgcagcgac cgctttcgcg tcatattaat gcctccatgc gggaatcatc
     1081 gcagcgccag ggattacttg tggaggcact ggacggaata gaaaccctga agctgaataa
     1141 tgcagttaac tgggtccagc gccgctggga tggatataca gccagtgtcg cccgctcgtc
     1201 catacgggta aaaaatacca gtaatgtcat aatctgcttt tccacagcag ttcagcaact
     1261 gaatactgtt ctgctggtca ttctggggac gtaccttatt cacagtgatg acccacataa
     1321 ccgaatcacc atgggggtac tgattgccgc agttattctt tccggtcgtg cgctggcacc
     1381 gcttggtcag atagcatcac tggccacccg ttttcagcag gcccgcctgg ccatgcaggg
     1441 ggtgaatgac attgtccgcc gtccggtgga gcgggaaagt cagacacatt atctgacagt
     1501 gaaggatatc cagggagagt ttcgtttcag tgatgtctcc ttttcttata agcccggatt
     1561 acccgacgca ttgtgcggtc tgtcgctctg tatccggccc ggagagcggg tgggtattat
     1621 tggctcaata ggttgtggta aatcgacgct gctgaaaatg atggtggggc tgtaccgtca
     1681 gaacagggga caaattactc ttgacggggt tgcgatgcag caactggacc cctgttttgt
     1741 ccgggataaa gtgtttcttc tggaacagaa tccccggctt tttctgggaa ctctgcgtga
     1801 aaatctgcag ctttcgcgtc tgccgggaag cctgtcggat gtggatatga ttaatgcagc
     1861 cggacgtttc gggtttgacc gttttatccg gcatcatccc cgcggacttg atatgcagac
     1921 aggggaagga ggtcaggggc tgtcaggagg acagaggcag atggttgccc tgacgagaat
     1981 ggtactgggt tctccccggg tggtactgct tgatgagccc acgacaggtc tggacctgca
     2041 gactgagtct gttgttctga aaggtctgag ccactggagc cgtggacgca caatggtggt
     2101 ggtgacacat cgtctgcagg ttctgaatat tgttgaccgt attgtcgtga tgaatgaagg
     2161 gcgggtaatg atggacggtc cccgtgatga aatactgaaa aaactgtcag ggaaacagca
     2221 gagtggggag agcagagcga taacttcatg agcgtcaacc ggataagaaa aaagagcaga
     2281 tatgtttaac agtttgtgga ccgcagggat atgacagaaa aaacggaacg gaataaagcg
     2341 tcgtcagagg tggtcaggca gtcagacctg gcgctgttga cagatgtaca ggcagccctt
     2401 cagcaggaaa agcatcatgc ccttttcctg atggtgattt ttctgctggt actggtagtg
     2461 acgtttgtta tctgggcatg gaacagtcct ctggatgaag tgacccgagg tcaggggagt
     2521 attattcccg gcagcaggga gcaggttata cagacccttg acccgggtat actgaaatca
     2581 cttgaggtca gggaaggcga tattgttgag aaaggtcagg tactgctgac actggatgat
     2641 acccggagct ctgccatgct gagggaaagt gaagcccggg tgaataacct ggaggccgtc
     2701 agggcaagac tccgtgcaga agcttattcc gaatcactga catttccgga taacgttcct
     2761 gaggatttgc gggagcggga aagtacggtc tattttctcc ggaagacgga gctggcgcag
     2821 agcattgccg gtctgaagca gagtaaggcg ctgctggaca gggaaatagc aatgacgcgg
     2881 ccaattgtgc gtgaaggggc aatgtcggag gtcgaactgt tacgtatgca gcgtcagtca
     2941 gcggagctgc agttacagat ggatgagaaa cagaataaat acctgacgga agccggtgct
     3001 gaactggtga agacagaagc agagcttgct caggcaaaag agaatatggc tggcagagcc
     3061 gacccggttg agcgttcacg tctcagggcc cccctgagag gtatcgtaaa aaatatacgg
     3121 gtaaacacac tgggaggggt ggtttctgca ggacaggaca taatggaaat tattccgctg
     3181 gaagaccagt tgctgattga agcttatata aatccacggg atgttgccta tgtcagaaca
     3241 gggatgccgg cacttgtaaa actgacggct tatgactatg ccatttatgg tggtcttgac
     3301 ggcgtggtga cactggtaag tccggatact ttacgtgacc agaaacggcc gggagattta
     3361 aaactggacc cgaatgaagc ttattaccgg gtactggtta ccaccagtaa taattatctg
     3421 acagacagga acggaaaaag attgccagtt attcctggca tgatagccag cgtggatata
     3481 aaaaccgggc gtaaaacggt atttcagtat ctgattaaac cgataaccag aatgaagcag
     3541 gcacttcagg aaagataata aaaaggtctt ccggggaaag tattttaaat caattttaaa
     3601 cgggcagaat gatttcactt tattctgccc gggtcattgc gcatgcctgt atgtgatttg
     3661 gggctgaaga taatataaat gttatatctg tcaggggaaa ataaatctga aaaaagcgga
     3721 atgattaagg aggtccggtc agagaaataa attatctgta atctgattcg tttttattta
     3781 atgactccct ttagcggata aaacataaga gcatactggt tttattaacc tgaggatact
     3841 tatatgaaaa tgattttcac cggcaaggtc agtggagaaa aaacagtact gacagccgga
     3901 gcccgtcata cagtaaaggc acaggccggg gaacagtatg gactggttga tgaggtgaca
     3961 ggtctggtcc cggatggtgt ggaagcggac cgttctggtg atgatttaat tctgcgtaaa
     4021 aaggaagatg atacagaaat ccggattgag ggattctggg aagaatgcca gccgggtgaa
     4081 acacagtgta cagcagtatt taatgttgtg ggtgaaaatg gtcaggtaac tgaagctgtc
     4141 ctgacccagg atggccctgt actggagaat ttcactgcag gacaatccgg gaccttgtca
     4201 gatgatgatc gtggcggttt tatatggttg ggcggtctgg catttggtgg ggggcttgct
     4261 gctatggctt tggctgcagg tggtggtggc ggcagcaagc acagacatga aaaccatgag
     4321 gcagatacaa cagcaccgtc agcaccggaa ttaacggccg gagataatgg ttcagtttct
     4381 gttgaactgc cgggtgatgc aaataaaggc gatactgttg aaatcacctt tgaagatgaa
     4441 aatggtgatg aacagaaagt cacactggag aaaggggata acggctggac atccagcaat
     4501 cctgcactga tcccggacag ccagggtgat acagccacaa tcgccccaga cactgttaaa
     4561 gataacagtg aagtcacagc agttgctaaa gatccggctg gtaatgaaag tactccggtc
     4621 actgtgacgt caaaaactga tggtgtggca gatgctccgg tcctgaccat tccagaagtg
     4681 gctgatagtg tggccaatgc ggctgagctg aaagatggtt tacaggcaga agtcaagttg
     4741 ccggcaggaa cagtggaagg tgcggtcatt acactgacag tgactcatcc ggatcaaagt
     4801 accgaaaatg tgacacataa cgtcaccgga gatgaggtta ctgccggtaa ggtatccatg
     4861 gatattcctg aggatgctgt tgttgacggt cagaacagtg tgagtgtcag cctgacccag
     4921 ggcagtaatc cggctaaagc ggggaataaa gtggagtttg tggttgacgg gcaggttccg
     4981 ggtgacacca atggtgatgg tgtggcggat gtcacaccgg cagtggccat tccggaagcg
     5041 acggacggtg tgaatgccaa agaactgaag gatggcgtac aggcagaagt gacggttccg
     5101 gcaggcagtg ctgaaggtga ta
//
LOCUS       DAZGTJ010000192         4984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000192 DAZGTJ010000000
VERSION     DAZGTJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.193"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(690..1133)
                     /locus_tag="ACHK8E_005034"
     CDS             complement(690..1133)
                     /locus_tag="ACHK8E_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347149.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNYEDDRK
                     SSGVSVTSLNSKKESVYYCKSIGIDRLSDLSAKMACDKYSALGCD"
     gene            complement(1120..3264)
                     /locus_tag="ACHK8E_005035"
     CDS             complement(1120..3264)
                     /locus_tag="ACHK8E_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFP0347150.1"
                     /translation="MLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQS
                     GTFVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSR
                     HDDSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAG
                     ELFWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFL
                     GAQDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLY
                     LRNGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQ
                     LSQHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYD
                     SLIHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTR
                     WSSVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFP
                     RTPINGVLTPIEFIHFYNGDKIDDTDYEEAAKELGCEVAAIKAVAKTETGSYGSYFKF
                     EDNDDYVPAILFERHHFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKK
                     AALKSASWGKFQILASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSI
                     RTKDWLSFAQRYNGPRQKGYDLKMERNYNASL"
     gene            complement(3773..3987)
                     /locus_tag="ACHK8E_005036"
                     /pseudo
     CDS             complement(3773..3987)
                     /locus_tag="ACHK8E_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3993..4679)
                     /locus_tag="ACHK8E_005037"
     CDS             complement(3993..4679)
                     /locus_tag="ACHK8E_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0347151.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAMVDTLNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(4791..>4984)
                     /locus_tag="ACHK8E_005038"
     CDS             complement(4791..>4984)
                     /locus_tag="ACHK8E_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347152.1"
                     /translation="YSEPVHGIQGYNCYDGGEHTDSDLQAEWPGRETLPQPRLYLVEE
                     CTEEQTKAAPLPVPLVSAQ"
BASE COUNT         1314 a         1231 c         1036 g         1403 t
ORIGIN      
        1 atccaaacag ccccatactt caggttaaaa agaacaataa aaaatgatgc taaaattgca
       61 acattctcta aataaattca attaattatc taaacaaggt gaaactttca gaggaaatga
      121 gcgcggcccc ccctaataat cttaagctgg gtacagggaa gtatcgacca ttacgcagtg
      181 tcgatatcga atcgtacaaa aaacagtctt ggcgtgggat atttacacat aattattagc
      241 tcacaatctc cctatacctg tcgtgttttc tggggattcc tcagggagtg accatagatt
      301 ccgttttcca ggtgcccccc tttttctcca aaaattgaaa aataactctg tagatgccgc
      361 taacaatcaa ttcgagttga tattttaaag cgttctgtgc agaaagaaat aaatttcaaa
      421 aatgaatcag gggagaaaag tgcgcttttt aacctgttca gtgtccagaa cacatgaaac
      481 cgatcgccat tcagtttcat gcatagtcat gcaacgcact gaaaatgatt ctgacgcatt
      541 tttccggttt cccccgagga aaacatccct ttttgcgtca gaatcgcgct cagcgcgttt
      601 tagcggcacg tacaattaca ggattataaa tcgtcaccag cgactggcgt aaaaaagccc
      661 ctctctgcag gcgagattta gatgttttat taatcacacc ccaaagctga atatttatca
      721 caagccattt ttgccgacaa atcactaagg cgatcaatgc caatggactt acaataataa
      781 acagattctt ttttgctatt caggctcgta acactaactc cgctgctttt tctatcatcc
      841 tcataattac taaatatcgt gtacttatac tctgagttta caaacgatac attgaaataa
      901 tcggtttgaa atcgtgagta atggttgtat ttaaaccctg aataatcact acctttcgat
      961 tcataattaa attcagtttt gtggtctttc gataaggtgt accgaagtac acctttgttt
     1021 tcatctagtt tgattgttcc tttttccgtg ttgcataaaa aatacacatc acttgcaaac
     1081 gatgccccac acagaagcat tgcaatcaac gcaaagaact tataatgatg cattgtaatt
     1141 tctctccatt ttcaaatcat atcctttctg ccttggtccg ttatatcttt gagcaaatga
     1201 aagccaatct ttagtccgaa tagagcgcag taatactcta tcagccttaa tgaagctaac
     1261 gaatgctttt aactgatttt tctcagactt acttaatgca aaaacgaaat cctcagggct
     1321 cgcgtagcct gctgtggcgt agttcgacgc taaaatctga aacttacccc aggatgctga
     1381 ttttaaagct gctttcttgt ctaaagtata cgctttaact agctttgcat actgtattga
     1441 tgttgcacca tacccacccg caacagggtt tgatatatct tcaaactgat catacttccc
     1501 atttgtatat ttatgaaaat gatgcctttc aaataatatc gctggaacat aatcatcgtt
     1561 atcttcaaat ttaaaatagg agccataaga accagtttct gtttttgcta cagctttaat
     1621 tgctgcaact tcgcatccta attcttttgc tgcctcttca taatcagtat catcaatttt
     1681 gtctccatta tagaagtgaa taaattctat aggagttaat actccattaa taggagttct
     1741 gggaaactct gcacttcgta atatttccgg aaactcaagc ggatgaaaat gccatacaga
     1801 ccgctgactg gcgaacggag gcaccttatc catccagtgt gttgcatcca gaaatccccc
     1861 gttagccatc ttcacaaccg ggctttcatc cctggactta cataccgatg accagcgtgt
     1921 atcatcgcgg gtactgtgcc agcagctgtc gtgtttcact atcaggcgtc gcgcatattc
     1981 cgggatgttc atctgattgg tatgcagcgc accaaacaaa tactggtcca gagatttatc
     2041 gggatacggg tttggcgctc ttctgttttc agcgttgtgg atcaggctgt cataaaacat
     2101 ttttgcctgc gcgaactgcg gtcctttttc accgtcaaag tgcgagctga tgcttttcag
     2161 ggcatcaatg agccagcgtt catccagcgt gcgggtaaaa tctgttgtgt gttcagcttc
     2221 aatacagtga aaatttagcc ctgctaaatc gtgctgactg agctgctcca catcgttctg
     2281 gtgcatccac gtatgtgggc gaatctgaaa gtatgtgatc ccggttttat ccgtaaacgg
     2341 gtacgtggca tcacgtggga taatttttcc ggcatcaatt cgggttatgg cgctcatttg
     2401 ttcaaatgta ctttcttcac cctctccatt tcgcaggtac agcgggcgtt tcagcttcag
     2461 cttaatgaac tgtttgcctg ttttaatccc tttcatgttt gccaccacat ctggcacatg
     2521 ctcatcgaga ctcagtagct cgatatgggc tttatagtcc gtggtaataa tttgcggata
     2581 gttatcttcg tcagccagat cctgagcgcc gaggaatccg accgcatccc ctgctttcac
     2641 catcactttc agtggcacag gcggcaccac cacatcatca aacacgccgt gattcagcgc
     2701 actgtgcatc cagtcaggca ggcacacgta acattggcca tcaggttcca gatattccgg
     2761 acgcatcgag acccagaaaa gttctccggc gggtttaccg tcctgaagac gttgcaccag
     2821 agcaaacgac tccggccgct gtttcagtaa aaacgatttt tgttgcagta cggcaagcgt
     2881 gtcaccctgc gtcagtgttc tggggtggcc gtgtttgttt gccagtacgt ccggcgcgat
     2941 ttcacggctg tcatcatggc gggaatattt acgcatccgc acaccgtggc ctttttgtgt
     3001 aacccggtaa agcgggcgtt tcggaaattc tgacaatggc gcgatgtgca tgtaaagctg
     3061 gtacagcgtc agccaggatg attcatcctg ctcgtctggc ttgtacactg attttaccag
     3121 cacaaaggta ccggactggc gcagggggcg ttcctgataa cattcgatag ccgcgtaatc
     3181 gcggttaacc cgccatgcca ccacttcgcc atccatcatg aactggagcg gcaccgattt
     3241 ttcaggtgtt tcctgattca gcaccgaggc cggggacgat gacctcccga catgaatacc
     3301 tccgtgccag taacgattta cgccaagtag ccaccaaccg tgggcgtttt tgcggatgtc
     3361 ggttagcagc tcgtcatagc tcttatagtc tcgtccatcg gctttccgta ccggataacg
     3421 tatgttcatc atcttttctt aagtgaaatc acatatttcg atggattctc gcgcgtattt
     3481 tgggtttggg gtgtagtgga accaaaaaga cggttaagcg gttaaattga atggtcaata
     3541 acaggtaaat caatctgttg agatcaacga cgtcttttgc tccgcaaacg gctaagatga
     3601 gtacagataa tttggttatc gtaaaaaagg tggctaaaaa tttcaacctg caatgtaaat
     3661 tagccggttg atttttggct atttaaattg acctcttaac ggcgtttatt gttccattgc
     3721 tccccaaccc cctatggatt ataagccgca catacattac ttcgttctgc atatcaccac
     3781 taatctgctt aagatgtact aacaacatca tcgcctgttg gctaaacgga actataactc
     3841 ctctttcatt ttcatgccgc gatacaagta atgcacacct ttaatttctt cacgttttgc
     3901 cggaatgcgc caaatagcgt tatcaaaatc aaattctttt taacgagcaa aacgccagtc
     3961 cgaagactgg cgcagtgata aaacattctg catcatgcca cattctgctc ctgaagatga
     4021 cgcagacgat ggtcccagtt tcccaggtaa tgcccgggag tcagcagtac gcggcgtcgc
     4081 tcattacaga gcgagttacc aatcaccact tctccggcca caccacacag cgacagttgt
     4141 atatacgcca tgccggctgc cagcgggtca atatccgttg ccgacaccca catcctgtgg
     4201 tatgccggat accccgaacg gttcagggtg tcaaccattg ccagaatcat actgccggca
     4261 ccacaggcag gttcactgag cgtgataaag ggtttactct caaacaacgc cttcacattt
     4321 cccagttgca tctgtgccat catgctggca acactccagg gcgtgaaaaa ctgactccgg
     4381 tatttatccc ccagttccag ttgcatgaac acactaccca gaaaatcaca gaatccctgc
     4441 tccagtccct caatgacatg agcaagcaaa tgcgcaatgt ggataacatc ctgcttttca
     4501 taaccactga cgattcgcag atacttctgt tcccgggcct cactgaactg aagccggttt
     4561 tccagggcaa tcacactaca gctgacaaag tcttcaaaca ccttatgacg tttgtgataa
     4621 caggctgtcc ggttaaacaa cgaaatgaat tccttctgat ggtcagtcat ggtggccatg
     4681 ttttctccgt aaaaacaggg aacacatggc ccggacaggg gaacaggtgt tccccttcgg
     4741 gttgaaatgt tgataaaacg ggcatcacgg acataatacc cggaggttaa ttactgggct
     4801 gagaccagag gaaccggtaa aggtgcggct ttcgtctgtt cttcagtgca ctcctccacc
     4861 agatacagtc ggggctgtgg aagtgtttcc ctgcccggcc attcggcctg cagatcactg
     4921 tccgtatgct ctccgccatc gtaacagttg taaccctgta taccgtgtac cggctcgctg
     4981 tacc
//
LOCUS       DAZGTJ010000193         4842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000193 DAZGTJ010000000
VERSION     DAZGTJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.194"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            57..776
                     /locus_tag="ACHK8E_005039"
     CDS             57..776
                     /locus_tag="ACHK8E_005039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347153.1"
                     /translation="MGCFATYITRQGGVLIPRNTYLVRTAYETAEEANDYGEFPQRIY
                     GVRTPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFDVECGFAAPWTRGNNCPPDQN
                     LTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTERKQLTRQLKNPPPDTETVKNG
                     GISAPAGGVRVSVALTAEPHITAQITRELDALGVQLHESAVVSVRHGARVRLADGRTA
                     YWNDSTRRIDITPAPNTEAPQ"
     gene            773..1177
                     /locus_tag="ACHK8E_005040"
     CDS             773..1177
                     /locus_tag="ACHK8E_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347154.1"
                     /translation="MTRFYCAMQRQLFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSAASRQSRR"
     gene            1226..1381
                     /locus_tag="ACHK8E_005041"
     CDS             1226..1381
                     /locus_tag="ACHK8E_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347155.1"
                     /translation="MNDTRRISDRELTKMIESAQQLLSRQNPVVEETWWEDLLAALTE
                     LRERRQQ"
     gene            1455..1619
                     /locus_tag="ACHK8E_005042"
     CDS             1455..1619
                     /locus_tag="ACHK8E_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347156.1"
                     /translation="MNELFIAVKGALIFTIAWIALMAFLTWENPFRHFTPGFIARIYA
                     ATVAVIWMVC"
     gene            complement(1714..1977)
                     /locus_tag="ACHK8E_005043"
     CDS             complement(1714..1977)
                     /locus_tag="ACHK8E_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0347157.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTLELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(2015..3067)
                     /locus_tag="ACHK8E_005044"
     CDS             complement(2015..3067)
                     /locus_tag="ACHK8E_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0347158.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVDAGHVAEEKAVPVG"
     gene            complement(3064..>4842)
                     /locus_tag="ACHK8E_005045"
     CDS             complement(3064..>4842)
                     /locus_tag="ACHK8E_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0347159.1"
                     /translation="KEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQWAALLPAE
                     SVEEVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAEIAATRPAV
                     CDDGVAATSGGGEGKKRRYRKNNVSGLTAEMLDAWARKNLYAYQLHCRENRHREWRFI
                     LKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFALQFGITLTGK
                     HIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMATHDKYRTTY
                     FSTPSAKTHQAYLVWNGDEWRGDDPARRAVEFPKESAMRVGCECPDGIWRYIIRLEEA
                     VAGGLSARVNIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEVEMETWGDYD
                     PTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPLHKGERFRVLAVWQWQGFNFSWQAE
                     QIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNRLVMKMIDVV
                     AHKRIEWAKDAIDEASRERTEIPASFMAIRRTTTKSGNALTFVAERSDVTGHADVFFA
                     ISHAVINEPVDYDYERPSTWAFGKAA"
BASE COUNT         1112 a         1487 c         1272 g          971 t
ORIGIN      
        1 ctgccccgca gcgtctggac catgacgaca ttgacgccat actggccgcc gccgacgtgg
       61 gatgttttgc cacctatatc acccgccagg gtggtgtgct cattccccgc aacacgtacc
      121 tggtccgcac cgcatacgaa actgccgaag aggccaacga ctacggcgaa ttcccgcaac
      181 gcatttacgg cgtccgcacc ccgtcactcg gggagcgcta caccatatgc acccacccgg
      241 acacctggaa actggtcaga cgtaagccgg aaaacgaaga ccgcacggac gagggttttg
      301 acgttgagtg cggctttgcc gcaccttgga ctcgtggcaa taactgtccc cctgaccaga
      361 atctgaccat aaacgacccg ccagaaaggc agccggacgg acgcagggag cagagactta
      421 ccctgccgga aggtgatttg ggtgaatggt taaggtcact gacccctaca gagcgaaaac
      481 aactcaccag gcagctgaaa aacccgccac ctgacacgga aaccgtgaaa aacggcggca
      541 tcagtgcgcc cgctggcggc gttcgcgtga gtgtcgcact gaccgctgag ccacacatca
      601 ccgcacaaat cacccgcgag cttgatgcgc tgggcgtgca gctgcatgaa agcgccgtcg
      661 tttccgtccg tcacggtgcc cgcgtgcgcc tggctgacgg acgcaccgcg tactggaatg
      721 acagcacccg ccgcattgat atcacccccg caccgaatac ggaggcgcca caatgacccg
      781 cttttattgc gccatgcagc gccagctttt tgacgaaatg atgcgccagc gcacgccctt
      841 actgaccctg aacaacattg ccacggggcc acaaccgggc gatgagctgg aaatcacggt
      901 cgtcagtccc gatcgcacca ccacggccat catcaccgac atactcaacg gacgaccgga
      961 cagcaccaac cgcagctggc ttgtcgccgt tcgtccgcgc cgtccgcccg gagagccgga
     1021 gccgctcacg cctgccggaa aaatcatgcg gcagtaccac gaagagcagg cacacaaaca
     1081 caacgcagaa ctcaccagaa cactggtcac gattaaccgc aaagcggcaa ttgcccgcta
     1141 tgtgcattca gccgccagca gacaatcacg caggtaaaca aagaccatga acatgaccag
     1201 aaacagccaa ctaaaggagc gcacaatgaa cgacaccagg aggatcagcg acagggagct
     1261 tacgaaaatg attgagtcgg cacagcagct actttcacga caaaacccgg tcgtggaaga
     1321 aacgtggtgg gaggatttac tggcagcact gaccgaactc cgggaacgcc gtcagcagta
     1381 aggctgcgct ggcgcacctg attttttcac cgcaacgcaa tttattcgcc tgacacacaa
     1441 aggaggacac cacaatgaac gaactgttta tcgccgttaa gggcgcactg atttttacca
     1501 ttgcatggat tgctttaatg gcattcctga cgtgggaaaa cccgtttcga cacttcacac
     1561 cgggctttat cgccagaatt tacgcggcaa ccgttgcggt tatctggatg gtatgctgaa
     1621 agaaaaaacg aatgatatga agaaccactg gagctggtga gtcggggcaa aaattctttt
     1681 tgccccggcg aagcagcaga caagcgaagc gcgtcaggaa cgccgcgaca gaagttcaag
     1741 cgcataccgg cgatcttccg ggcgcatggc atccaccagg aacttcacca tattgcgacc
     1801 ggagcgggca ccggggctga gggtatggga aaaggtcatc gtacagacga aggtgtgccc
     1861 gcattcgaca tcactgcatg cacagtaaag atccgcaagt tcaagggttt tccagtctgt
     1921 cttccggatg acagcaggtg cgccacagtc cggacaccgg gctttaaaaa tccgcatctt
     1981 acctcccgat aacacgtatc ccggggagga cattttaccc gaccggcacc gctttttctt
     2041 ccgccacatg cccggcatcc acatcaaaac aaagataaag gtgttccggt atatcgggat
     2101 cgctgttcac ggcgttcatg attttacgct gaagcggtat cacctcatcc ttgcggtacg
     2161 tttcacgcgt cgtctccggg ttagggatgg tggccccgtt cgtggggatg atccccgcaa
     2221 gaccggcagg aaaacggtgt gccgtaaaca catcctgggc gctgatcccc ttgatgtttg
     2281 aaaattcatc cttggcgctc acctcaccaa tcggaataat tttcacccca tccggcgaac
     2341 ctttggggat gttgatgtac agattgcgga aatttcccag ccccttagac tccgccacct
     2401 tctcgcggat ctcatcctcc atctccgtgg tgatcccagg gtcgttcgta tacagcaaaa
     2461 aacccatatg cgccccgttg ttgtagtaac ggcgacgaaa aaggctggcc tcgctgttca
     2521 gcagcgccga gtggatgcca ccgatataat ccggcagccc gtaaatttgc tgacgcggat
     2581 catacatccg cagaaagata atatcttccg gcgtatagac cagcgcgggg ccttcctgaa
     2641 gcaccacgaa ctcaccggtt ttgcggcgcc ggagatacag cgacggaagc ggctcaagcg
     2701 ccacgacgtc gccgaatacg ttacgcacct tgagcagggc gagatcgccg aacaacaggt
     2761 agtcaaatac cgcctgttcc atctggtccg ttgtgaggcc accaccggta tagccccccg
     2821 ccaccatgtt acggcgggca tacaacaccc caccgtgctg ggcgttaagg ttcgggagct
     2881 gcgccagtgc caggcggtta atcggcaact gccagtggtt gtgttcgttg tcataccaga
     2941 tgttatggta gtccgtgccg gttgtcagca ccggttccgg ctggccaaag gtgattgtgc
     3001 tgccacgtgc gggcgtgaat gcttttttcg tcacgcgtga cgttttgttt ctgcgtttct
     3061 tgctcatgcg gctttcccga aggcccatgt ggacgggcgt tcatagtcat aatctacagg
     3121 ctcattaatc acggcgtgcg aaatggcaaa aaacacatcc gcatgccccg taacatctga
     3181 ccgttccgcc acgaacgtca gcgcgttacc gctttttgtg gtggtgcggc gtatggccat
     3241 aaacgacgcg gggatctccg tgcgttcacg gctggcctca tcaatggcat ctttcgccca
     3301 ctcgatacgc ttatgggcca ccacgtctat catcttcatc accaggcggt ttttgctctc
     3361 cacgctgtaa agaatggcgt tagcctcacg cggcgcaaag cgactgacga tatcaaacac
     3421 ccctttcccg atgccggtaa tatcgatgcc gatataggta atgttaaagc gctgcatcag
     3481 ttcgcggatc tgttcggcct gccagctgaa attaaatccc tgccactgcc agactgcgag
     3541 cacccggaaa cgctcgccct tatgaagcgg cggagccaca atcacgaacg tggaattatc
     3601 cccggacctt gacgggtcaa agcccgccca cacctcacga ttgccgaacg gacgtgctgc
     3661 ggtggggtcg tagtcccccc acgtctccat ctccacctcg cacctgacca gttcggagaa
     3721 cttaaatacc gcatccttgc tgtcaacaaa ctcgcagccg tacagcatcg cgtaggtggt
     3781 cgggttatag cgattcctga tacgttcaat attgacccgc gcggacaggc cacccgccac
     3841 cgcctcttcc agacggataa tgtagcgcca gatcccgtcc ggacattcgc accccacacg
     3901 catcgcactt tctttcggaa actcaaccgc acgacgcgcc ggatcatcgc cgcgccactc
     3961 gtcaccgttc cacaccagat aagcctggtg ggttttggcg ctgggcgtgg aaaagtacgt
     4021 cgtgcggtat ttgtcgtggg ttgccattgc cgaggccact tcatgcagtc gcgtgaattt
     4081 tgggatccag aacacctcat cgccgtaaag gtggccgtta aatccctggg cggtactggc
     4141 attggtggac aggaaacgca gcattgcgcc attgcttaaa cggatatgct tacccgtgag
     4201 cgtgatcccg aactgcaggg cgaaattaat aatatattcg cggaagattt ccgcctgaac
     4261 ccgtgacgca gaaaagaata tctggttatc accagtcacc accgcatctt cgaacgcctc
     4321 ccatgcaaaa taccaggtca tgcctatctg gcgactcttg aggatgaaac gccactcacg
     4381 gtgccggttc tcgcggcagt gcagctggta ggcataaaga tttttacgcg cccatgcatc
     4441 cagcatttca gcagtcagcc cggaaacgtt gtttttccgg tagcgccgct ttttcccttc
     4501 ccccccgccg ctggtcgccg ccacaccgtc gtcgcacact gccggacgcg tggcggcaat
     4561 ttccgccatc ctttcagcgt gtttgttttt ctgggcgata atcttcacgt gctgtgcgat
     4621 aagctcgcgc agttcggcaa gttcgcactc cgtcttaccc tcgcgcccgg tcagcacatc
     4681 aatacgccgg gcgatcacct cctcaacgga ttcagcgggc agcagggcgg cccactgccc
     4741 cacatcacac cagtggtaaa ccgtgcgcgg cggaataccg agctccgccg caatatctcg
     4801 gggatgccac cgcttcagat aaagcgagcg ggccgcctct tt
//
LOCUS       DAZGTJ010000194         4812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000194 DAZGTJ010000000
VERSION     DAZGTJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.195"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1123
                     /locus_tag="ACHK8E_005046"
     CDS             <1..1123
                     /locus_tag="ACHK8E_005046"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0347160.1"
                     /translation="IMAKLYLYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLS
                     ISGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANNLNMLLLGRILQ
                     AIGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVI
                     FIVMGAFSILTILLSPKESNKQEKKTHQDNVNFVTKDFICGTILVSLMLSSLFCYISI
                     SPALFMSDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISNPWILFPLNAISVTPFLI
                     LNDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISF
                     LSVKISDNGVSLGTTLSISCLILALLSIFVTIIIGNNKK"
     gene            1120..1950
                     /locus_tag="ACHK8E_005047"
     CDS             1120..1950
                     /locus_tag="ACHK8E_005047"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0347161.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEERLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKIPYFILEKTAHATLQINRGLLQQFGEI
                     IRIDFQHKNDIEKAFKYASINNMIPISISDSVGSMGGVAPVEFIVRMANRFDGYAYID
                     DAHGTSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGLVLMVALLHAIILTP
                     "
     gene            1978..2331
                     /locus_tag="ACHK8E_005048"
     CDS             1978..2331
                     /locus_tag="ACHK8E_005048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347162.1"
                     /translation="MLLVVHHHYQELLHVSQQQIYTSMAQYPCVKPCVKMTYNNINKG
                     TTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENI
                     DSLHKNFIECINELG"
     gene            complement(2401..2583)
                     /locus_tag="ACHK8E_005049"
     CDS             complement(2401..2583)
                     /locus_tag="ACHK8E_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347163.1"
                     /translation="MQFRLKGCQDTLFAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQ
                     HRQQQRRPGHYRGMSM"
     gene            complement(2857..3351)
                     /locus_tag="ACHK8E_005050"
     CDS             complement(2857..3351)
                     /locus_tag="ACHK8E_005050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0347164.1"
                     /translation="MSEFYSRAATVADMPFIMGEFEDGARKGHFYEEILTSKVGDTFE
                     QQLRLEIKANEQGQYTGHFIYILLRRSDDKKVGLIWFCDVRDPTGVPRLELRAVSIVK
                     ELRGKGYGSMLVSDMLASNPSRPMMARCYVKSSQMADMLKRRGFYLFGTSPKGTQMLF
                     RDPK"
     gene            complement(3501..3779)
                     /gene="yacB"
                     /locus_tag="ACHK8E_005051"
     CDS             complement(3501..3779)
                     /gene="yacB"
                     /locus_tag="ACHK8E_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFP0347165.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(3779..4048)
                     /gene="yacA"
                     /locus_tag="ACHK8E_005052"
     CDS             complement(3779..4048)
                     /gene="yacA"
                     /locus_tag="ACHK8E_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFP0347166.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEVGRQQLERGDVLSHDMVESSATAWRDEMSRKVAGK"
BASE COUNT         1389 a         1033 c          984 g         1406 t
ORIGIN      
        1 aattatggca aaattatatc tttaccttgt tcttgctgca tcattatcgg cattatcaac
       61 tgacatgatt gtgcctgcgt taccagaact acagaaagca tttaatgctg actactcatt
      121 aattcaacta agtattagtg gatttcttat tgtatttgca tttagtcaac tgatatcagg
      181 aaccataggt gattattttg gaaaagtacg cgtaatgact atatgcttaa ttatttttct
      241 tattggtagc attgtatgtt ttatggccaa taatttaaat atgcttcttt tgggtagaat
      301 tctacaggca ataggtgcag gtgccggacc tgtaatatca aaagcaattg ctaaagagtc
      361 ttttccacca ctaaagctaa aaagagcact ttctgacata tcatcaacaa gtgcgataat
      421 accattaata gcacctctgg ccggagcatt aatacttgat tattataact ggaatgtaat
      481 atttattgta atgggtgcat ttagcattct aaccatactc ctgtcaccta aagagtctaa
      541 taaacaggag aaaaaaacac atcaagataa tgtaaatttt gtgactaaag atttcatctg
      601 cgggacaata ctcgtttcac taatgctaag ctcactattt tgctacatat caatatcacc
      661 tgcactattt atgagcgatt atggattaaa tacatttaac tactctgttg ttttttccct
      721 gtcagttttg tttttcatca ttggaaacca attatcaaaa atcgaaaaaa tatcaaaccc
      781 atggatctta tttcccctaa acgcaatatc agtcacacct tttcttatat taaatgatgc
      841 ttttataata tgtgttattg gagcaatgat tttcaattta actcttggcg catactatcc
      901 aatagctaat ttcatctcat tacagataaa cggttcacga actggtttgg cagcatcaat
      961 tacaggcttt actcaaacag taagcgcagg gacaattagt ttcctttcag taaaaataag
     1021 cgataatgga gtttctttgg gaaccacttt aagtattagc tgtcttattc ttgcattatt
     1081 gtcaatattc gtaaccatta ttatagggaa taataaaaaa tgaattggca aaagaaaaag
     1141 atagaacgtt caatcaaata ccaaacaaga gtatttaatg aacatataaa tgaactagtg
     1201 atcaccagaa gaaatggtaa ggtaatcact gttaatgatg ggagggagat gattgagttt
     1261 gtgtcatgct cttatcttgg actggaaaca catgaaaatc taaaacatgc aatcgctaat
     1321 gcaattgaaa gatttggagt ccaactttcg gtcgccagaa cgcgcgtaaa agtagattta
     1381 ttcgagcaac tggaagagag attaaataaa ataatgaatg gggcgcacac attaacattt
     1441 aatgcggtta ctccatgcca tttagctgta ctcccacttc ttgcatcagg aacactacct
     1501 aattacaagt ttaataaaat accatatttt attttagaga aaacagcgca tgcaacattg
     1561 caaataaata gagggctatt acaacagttc ggtgaaataa tcaggattga ctttcaacat
     1621 aaaaatgata tagaaaaagc gtttaaatat gcttcaataa ataacatgat accaatatct
     1681 atatctgata gtgtcggttc gatgggtggg gtagctcctg tcgaatttat tgttagaatg
     1741 gctaacagat ttgatgggta tgcttacatt gatgatgctc atggcacgtc aatatatggg
     1801 aaaaatggct ccggatatgt aatgtcttgt ctggataaca aacttgatga cagaattatt
     1861 atcgctggtt cattatctaa agcgtttggt tctcatggtg gtttggttct catggtggct
     1921 ttattgcatg caattatcct gacaccataa actttataaa aacgtatgga actacctatg
     1981 cttttggtgg tccaccatca ttaccaggaa ttgctgcatg tatcgcagca gcagatttac
     2041 acctcgatgg cacagtatcc ttgcgtcaaa ccttgcgtca aaatgactta caacaacata
     2101 aacaaaggga caacaatgcc aatacgtgga ttgttaattg gcaatgaaga aaaagctata
     2161 acgatatgca aaaaaatgca tgatcgtggt tttgctacaa ccgttgctat gtatcctaca
     2221 gtggaagagg gaaaggcaat tctacgttgt gcaatatcag ctattcatac ccgagaaaat
     2281 atagatagcc tacataaaaa ctttatagaa tgtattaacg aactgggtta gtaaatatca
     2341 tggcctcttt atagagaggc catctatttc attttttatg gcgtatagct gtccctgaca
     2401 ttacatactc atgccccggt agtgtccggg gcgtcgttgt tgttgcctgt gctgttcgtc
     2461 ccgcttccat tcatgccagg tctgattaaa tccgagttga cggaagagat aatccggcat
     2521 ctcttccggt ttgttgatgg cagcaaaaag cgtgtcctga cagcccttaa gccggaactg
     2581 catcaggtgc ggttccggtg ggtacggagt atccgcatca tggtttaggg gtttgagggc
     2641 caaccgtacg aaaacgtacg gtaagaggaa aattatcatc ttaaaaatcg attagtagac
     2701 aatgccgtcc gttaagtgcc aattttcgat taaaaagaca ccgttttaat ggcgtttttc
     2761 tatgtccatt atgtttgata tatcagacag ttacttcact aacgtacgtt ttcgttccat
     2821 tggacttcag accccgccag catgggccag tatcttttac tttgggtcgc gaaacagcat
     2881 ctgcgtgcct ttgggtgacg tgccaaaaag gtaaaaacct ctgcgtttta gcatgtcggc
     2941 catttgcgag gatttgacgt agcacctcgc catcattggc cggctagggt ttgatgcgag
     3001 catatcggac acaagcattg acccatagcc tttcccgcga agctctttga caatactgac
     3061 ggcacggagt tcaagtcgtg gcacaccagt tggatcgcgt acgtcacaga accagattag
     3121 tcctactttt ttgtcatcag aacgtcgcag caggatgtag ataaaatgcc cggtgtattg
     3181 gccctgctcg ttcgccttga tttccagcct gagctgctgc tcaaaggtgt caccaacctt
     3241 tgatgtgagg atctcttcgt agaagtgacc tttgcgtgcg ccgtcttcga actcacccat
     3301 gataaatggc atgtctgcca cagttgctgc ccttgaataa aattcactca tgtattacct
     3361 cagtatcttt ctaagacacc atattttatg ccccggttaa aactatgagc aaaccagacg
     3421 gccgcgactg ttcagaacat atcgctctta ctgggggagc agtaaactaa aaatgaaaag
     3481 tattattcct gacaggaatt ttatggccac ttctgtgcgg tatgcaacac gcgcaggata
     3541 tacacttttc cccactggct gtcgacctca taaaccagaa caaaatgcgg gtgaataacc
     3601 agttcccggg taccttccac acgaccgttg cggcctttat acggctgccc ggccaggctg
     3661 ctggctgaat aaccaatccg ttcgtccagt tcaatggcag ccatcaggtt ctgctccgca
     3721 atgtactcac gaatgcgctt cctgtcctgg ctggccagca tggtccagaa tatctccatc
     3781 atttaccggc aaccttcctg ctcatttcat cccgccatgc ggtagctgag gattcgacca
     3841 tgtcatggga aagcacatca ccgcgctcaa gttgctggcg tcctacctca acctggtcac
     3901 ggaaccaggt attatgctgc cgttcaacgg tctgacgcat aaaatcccgg attaactgag
     3961 agccattacg gtccatgctt tttgcagcgg ccataaaagc atccttcagt tccgcgtcga
     4021 ttcttaaact catattaacc tgtgccattt gccacctcgt cattaccacg catataccca
     4081 gtatataacg attcagatat cgttaccaga atatgccgcg tcagcggcat ggaaggcggc
     4141 actctgttgt ttcatatgat accggagtaa aaccgccgaa gcccggcgta agccggtact
     4201 gattgataga tttcacctta cccatccccg cccttggcca tatctgcttt cagccatgat
     4261 aaatcttctg tgcgcggtcg gagtggtccc gacgagggtt tacccgaagt cggggcgtat
     4321 ctccgcgtta gcgggccgtc agggccgctt acgagcgtgt tctgagggct tccagccaga
     4381 agaggggttt gaagcccaat ggagcgggaa cgtacgctaa gaagctaact atatgatttt
     4441 aagtcaataa agtcgtggtg ttgttaggca ggtttacgtt gatttgatta ccgagtttat
     4501 taacactgaa agtccctgaa accgcatggc tactggcatt gacgggaaga gtaaacaaaa
     4561 ccctcgtttt ctttactcac ctcttctgct caaaaaatga acttagcgta cgatctcgta
     4621 cggttgggcc tcaaacccct ctactaatcg atttttaaga cgataatttt cctcttaccg
     4681 tacgttttcg tacggttggc cctcaaaccc cttatactca tgcgtgcggt atcaaaaaat
     4741 ctgcaattac cctttttgaa tgaagggggg agtcaactat gaatagcacc acacattacg
     4801 aaaacgccaa tt
//
LOCUS       DAZGTJ010000195         4809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000195 DAZGTJ010000000
VERSION     DAZGTJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.196"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1039
                     /locus_tag="ACHK8E_005053"
     CDS             <1..1039
                     /locus_tag="ACHK8E_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0347167.1"
                     /translation="ANATTLKPFSVALGAGSVADRGAGIEAYMPETKRWNPGDVVKSR
                     MSDKQLNRLMEIDKLISAYNSQETLSAEETKQLEEVKKERADMFATYIATHDAVSVGV
                     GATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVTEKADAALDKRITNNTSAIT
                     KNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSKQISTNTLGIREN
                     TQRVQDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRKRADAG
                     IAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAWDTENG
                     AGVSAGMALGW"
     gene            complement(1469..2044)
                     /gene="dolP"
                     /locus_tag="ACHK8E_005054"
     CDS             complement(1469..2044)
                     /gene="dolP"
                     /locus_tag="ACHK8E_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0347168.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
     gene            complement(2390..2617)
                     /locus_tag="ACHK8E_005055"
     CDS             complement(2390..2617)
                     /locus_tag="ACHK8E_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFP0347169.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            complement(3289..4380)
                     /locus_tag="ACHK8E_005056"
                     /pseudo
     CDS             complement(3289..4380)
                     /locus_tag="ACHK8E_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            complement(4643..>4809)
                     /locus_tag="ACHK8E_005057"
     CDS             complement(4643..>4809)
                     /locus_tag="ACHK8E_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0347170.1"
                     /translation="LPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAVYALSQYKT
                     EQKIREATGK"
BASE COUNT         1419 a         1113 c         1016 g         1261 t
ORIGIN      
        1 tgcaaacgca acaacattga aaccattctc tgtcgctctg ggcgcaggaa gtgttgctga
       61 tcgtggcgct ggtattgaag catatatgcc agagacaaag cgctggaatc caggggatgt
      121 agtaaaatcc aggatgtctg acaagcagct taacaggctt atggaaatcg ataagctaat
      181 atccgcttat aattcacaag agacgctgtc tgctgaagag actaaacaac tggaggaagt
      241 gaaaaaagag cgggctgata tgtttgcgac atatatagca acgcacgatg ctgtatctgt
      301 aggtgttggt gcaacgacaa caggacgaca tgctgttaca agacagataa ctagcgttgc
      361 agcaggttct gtcgatacgg atgctgttaa cgttgcacag ctcagggatt tgcagactgt
      421 gacagaaaag gcagatgcag cgcttgataa acgcataaca aacaacacca gtgcgataac
      481 taaaaatctg caggtggtta acagtaataa agaggaaatc agtaacatta ataaaacgac
      541 aaaaattctg gatgaaagag tgacagccaa tacacagaca gcagaagaac ataagctgct
      601 tatttcggag aacagcaaac aaatcagtac caacactctt ggtatcagag agaatacaca
      661 acgggtccag gatgaaggac aggctcgaat ccggggagac agggaaacac tgcgtgcagc
      721 gaccggttat accgacatgc gggtaaacgg gctggagcgt aacacgaata aacgttttgc
      781 acagcttaaa gaggatatag acaaaaacag aaaacgtgca gatgcaggta ttgcaggtgc
      841 gatggcaatg acggggatac cgtccgtccc cgggaaaaag gtctcatttg gtatggccat
      901 ggccggatac cggagtgaag gtgctgttgc tgccggcttc cattttaata caagtgaaaa
      961 cagtgctgtt aaagtgaatg cagcctggga cactgaaaat ggagctggcg tttcagccgg
     1021 gatggctctg ggatggtaat gcttccgggc cagaagccgt tcgcagatat tgttatccag
     1081 gggacactgc accaccattc aggaagcgat tcagtttatc gcatcggctc aggatgtaat
     1141 taatacggac aatctggctc ctgcaccgga gccagacgta cacgtgtcaa cgccacgatc
     1201 tttgacctgt atggataaaa gctcagatta tttaccagca gcgacatccg gagagcaccg
     1261 ggattttatc tgtgagtgaa catacccggg caaaaaaatc ccccgaattc aggggggtgt
     1321 caaagaggaa ttatgtatat tggttattat aatgagcacg tctccggtat tccgcgacga
     1381 atgttcacgt ggtggcatga acggcccgga aacgtgctct tctgattttt aacgtaaatt
     1441 tgcactaaag gcaactcaga tacggccttc accgtataca ggagaatgcc gtcgtgaccc
     1501 gcttcacgcc actcacccta ctggcaatat ctgctgccgc tcgcccttcc tgctctgtaa
     1561 ccaggcccag caaaaagact tcgctgtttt ctgtcaccac cttgacactg gatgatttga
     1621 cacgggcact tgtgagtaac tgagaacgaa tttttgtcgt gagccaggca tcacctgcca
     1681 ttatgaccgg actgacggga gtcccctgcc ggatttcatt atagacctcc tgaacaccat
     1741 cgacaccgag tgctatctga cgggcacggg cagataattc acggtccgga gactgaccga
     1801 tcagcagcac gcgtccctga tatgcaatct cactgatgcg tgcttctttt ctgatttgtt
     1861 catcttttga aagtgcactg ctgaccctca gctccagcac ttcatcatcc acctgcgttc
     1921 cgacactgcg tgggtcggtt gctgcctttg tcccgacagc ggctgtaccc accacagttg
     1981 cagcaacgca tccctgtaac aataaggctg aaatcaggac aacagcagaa tataataaac
     2041 gcatatctcc cccggaattt atcgtacaaa aagtttacct tcagtgaaac aaaacaagct
     2101 acggactgtt attctcttcc ggcacagccg gaaacagcgg atacgttacg tgacatatct
     2161 gtatctgcca gacggaaatg tttatgacgg atatggatgt actgaaattt aatattgttc
     2221 atgtttttgc acgaaaacgt tgatagcgta gtaaagattc tgttttttaa ctacctggtt
     2281 tgctgagcag gatctccttt tcgttataca caggctaaaa ggcaccgtgg ccccccggtg
     2341 cctttttcat aaaacagaga aaacacggct ttgaatatac cgtttatgat taccgttcgc
     2401 ccttttctga ctcatcttca aggattttct ggatttctct tttgatctca agtttttcaa
     2461 gcttcagcct gaccactttc gtcgaatacg ctgaaccaaa ttcatcttca attcttttta
     2521 tttcgtgatc aagtttatta tgcttttcaa ataaagaatc aaagcgttta tgatttcctt
     2581 tcaatgctga aatcagttct ctgtactccg ggaacattgt ttttatctcc tttcatcaac
     2641 actgtaacaa tacatataaa ctttaaattt attaaagatt ctcaccatca ttcgcagtga
     2701 ataatgccgc atctcatatt catatcctgc agagaaaacc cctccgaaga ggggcgagtg
     2761 tagcgttaat ggagtgttat gtagcatggt gccgggtgcc tcccggtgaa cagactgttc
     2821 gtgataccag tcagcaacat aggaaattaa tggtatcacc ccaccgcaca gggggattca
     2881 ccatgcctgc cttttttagc aaattcatca gaaaccggac aacatgctta tttatgaaat
     2941 gaggtgcttt tcacatttat ataaaatcat tataacgata atcctaaaaa tcaatgtaat
     3001 tatcatcttc agtaactttc tgtattacac catacggaaa ttgatttttg acggtaaaga
     3061 tacaagggga agtatgtctg taaggagtac cctgaataat ggacaggcat gcatcgtggc
     3121 tgttcccctg aaattatcag tacaaccagt cagctcataa tccctgtctc tttttccggg
     3181 gagccctcag ggggactttc ccttatcccc ccagtttctg gagagacgta aaaaagaatt
     3241 aatcatatgt caggtatctg aatatgtccc gtaattaata tatttacgtc aggatttttt
     3301 ctgcccgcca ggagtatgaa catcttccgc cgttgagtct gtcatatcca tttttttctg
     3361 tgccagtact tcatatgatt tttctttttc cagactctcc tgctgcatcc cgtgagccgg
     3421 agcatataca agctcagcca tcactggaaa atgatctgaa cccatatccg gtaaacggcg
     3481 taaactttta agcagaaaat gctggctgtg aaatacatgg tcaagcggcc atcgcaggaa
     3541 aaaataatgg ctgtgaaacg tgctaaaaaa accacgtccc cggcgtggat ccagcagtcc
     3601 acttattttc atgaataaac gagtggtcgc agaccacgca acatcattca gatcccccgc
     3661 taatattaca ggtaattcag aatctgcaac acttttcccg accattaaaa gttctgagtc
     3721 acgatcagtt gactcatcac tttcagtcgg gctcggtgga acaggatgaa gacaatgtaa
     3781 tcttattctc tgaccttccg gcatatgaac tatcatatgc atggagggaa tttccttgtc
     3841 cactaaatac tgtatttctg catcacttaa aatcatcctt gaatatacat gcatgccata
     3901 taaattttct ttcgggcatt tcagggagta tggaaacact tcatccagag gtgatagttt
     3961 ctgttcccac cataagtttg tttcaagaag aacaagtata tcaggttttt ctttatttat
     4021 aagttcaagc aaaggcatat attttttatt ggtcataagg acattgaata ccagtatact
     4081 taaacccgga aatccatcag agtcctttat ttactttacc tgcttcttgc caattactgt
     4141 ataaggaatt atccatacca actggtaagc gacacaaatt aaacttatta tcacaacaaa
     4201 caactctgca aatgagtcaa gaaaaacaac agacagaaaa acattcaaag tacacagcaa
     4261 aagtatctgt agtcggggaa aatcccatac cctgacaacc catgatgtat taccggaaac
     4321 agggataaaa gttatgactg ccagaaggat agcagtaaaa ataaaaacac aagttatcac
     4381 aaatcactcc ttgttctgaa ccggcacaca aaactgtcat atacgtttca aaagtaaaaa
     4441 cacactgctg ccacaagatt cacgacgtaa ccgggcagca tatcctgatt acggacaatc
     4501 catgaaaccg cctcgccaga agcgtccatc acatccggtt tttccctgtt ttatattcca
     4561 cgaaccattt tattttcagg aatctccggg cctttatccc gcatcatata cacagctatg
     4621 acaacccgct cttctgcagt catcatttcc ccgtggcttc gcggattttc tgttccgtct
     4681 tatactgcga aagcgcatac acggcccata ttgttgccgg tatccagccc agcagcgtta
     4741 tctgtaacac cagacaaaaa atccccgtca gaggccggcc gatggtgaaa aaagtcagcc
     4801 agggaagaa
//
LOCUS       DAZGTJ010000196         4787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000196 DAZGTJ010000000
VERSION     DAZGTJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.197"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(123..884)
                     /gene="kduD"
                     /locus_tag="ACHK8E_005058"
     CDS             complement(123..884)
                     /gene="kduD"
                     /locus_tag="ACHK8E_005058"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP0347171.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(914..1750)
                     /gene="kduI"
                     /locus_tag="ACHK8E_005059"
     CDS             complement(914..1750)
                     /gene="kduI"
                     /locus_tag="ACHK8E_005059"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP0347172.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2037..3218)
                     /gene="yqeF"
                     /locus_tag="ACHK8E_005060"
     CDS             complement(2037..3218)
                     /gene="yqeF"
                     /locus_tag="ACHK8E_005060"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0347173.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3473..4702
                     /gene="yqeG"
                     /locus_tag="ACHK8E_005061"
     CDS             3473..4702
                     /gene="yqeG"
                     /locus_tag="ACHK8E_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0347174.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1245 a         1207 c         1061 g         1274 t
ORIGIN      
        1 tcataaagtt cattaattga taattgatat ggattatttc ataaccatga tatggattat
       61 gatgatctac aggtataaaa aaccctgcca tgcggcaggg tcataaaagt aagagaaatg
      121 aattaacgag ccagccaacc gccatccacg gcaatggtat aaccattcac ataatctgaa
      181 gcgctggagg caaggaacac taccggcccc atcaggtcac tcggcagtcc ccaacggcca
      241 gctggaatgc ggtcgagaat ttccgcgcta cgttgttcat ctgcccgtag ttgttgagta
      301 ttgttggttg ccatgtaacc cggagctatc gcattaacat taatgttgtg tttagcccat
      361 tcattcgcca tcaatcgcgt cacgcccatc acgccgcttt ttgatgcggt ataagaaggc
      421 acacggatcc cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg
      481 ccttgcgcga taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc
      541 aggttcatga catcgtccca gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc
      601 aatccggcgt tattcaccag gatatcaata tgaccaaact cagctaccgc gcgatccagc
      661 agtgctggaa tgccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc
      721 agcgccgtga cctgcttgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca
      781 cagcctgctt gcgccagccc caacgccatc ccctggccca gcccagtatc acaaccagtg
      841 acgaccgcaa ctttaccttc gagagaaaat gcacttaaaa tcataacaat accttattcg
      901 tttatgccca caactagcgc aaatctttaa cggccacatg gtccatatca tcaaaaacct
      961 ggttttcacc gaccatgccc cagataaagg tataagcttt ggttccgacg ccggaatgga
     1021 tcgaccagct cggggagatc accgcctgct cgttatgcat cacaatgtga cgcgtttctt
     1081 gcggttgccc catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa
     1141 cttccatccg gcgctcgtgg gtgtgacacg gcatggtgtt ccacaagtta cctggagcca
     1201 gctccgtcag ccccatactc aattggcagg tttccagcac atccgggaca aaatatttgt
     1261 taatcgtgcg acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg
     1321 gtgtgacttt tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg
     1381 ccggagtggc ggtatcaata ctggcaaaga caacttcttt tgcaccttta ccaacataca
     1441 gggcttcgcg gtgaccgatt tcatagcatt gaccatcgac agtaatcgta ccggcaccgc
     1501 caatattgat aatacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac
     1561 caacttcccc gccaacggaa accgttttag ttaccggcat aatgccgcca acaataatac
     1621 ggtcaatgtg gctgtaaacc atggtgtact catcggcgac aaataccttt tcaaccaaaa
     1681 attcattgcg cagcccctgg gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc
     1741 ttacgtccac atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa
     1801 cgctgtttcg ttttctctat aaggggatga tatgcccacg ggatagggtt ttcaattaaa
     1861 aataaaacaa tgtttcattt ttaaggtaag gactgaaaaa agtgcagtgt cgatcacgaa
     1921 tatgtcatga gaatggaaaa atcagggagc cctgaaaagg tatgtcgact cgccagccac
     1981 cattattcaa cagattaatg acggctgata tttatcggta aggaggaaat gcagagctat
     2041 tcgtcacgtt caatggtcaa tgccacgccc tggcccccgc cgatacaaag cgtagccagt
     2101 cctttgcggg cattacgttt caccatttca tgaaccagag aaacgaggat tcggcaaccg
     2161 gaagcgccta ttgggtgacc gagtgcgatc gcgccaccat tgacattgac acgacgctca
     2221 tcccattcaa gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg
     2281 ataagatcga catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac
     2341 accggcgcaa ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcggatgcgg
     2401 gccagcacgg gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat tactgcagct
     2461 gcgccatcgt ttatggatga tgcattaccc gctgtcacag aaccgagact atcaaatgaa
     2521 ggatttaaac gggctaagcc ttctgcactg gcgtcagtgc gtggctgctc atcggtatca
     2581 acaaccaacg tctgaccgtt actttgggtc attaccggga cgatctcatc tttaaatcgt
     2641 ccagcgtcaa tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac
     2701 tgacggctga tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca
     2761 ttgaaggcat cccacaaccc atcgtgcaca agactatcaa ccaactggct attgccaagc
     2821 tgtgcaccgg tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca
     2881 ccggcgatga caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct
     2941 ttaagcccgg aaccgcaaac gtcattaata gtgattgcag aaacgctatt aggaagacca
     3001 cctttaatag ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca
     3061 agaattactt catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg
     3121 accaggctgc caagttccac ggcggaatga cccgctaacg caccacggaa gcagccgatg
     3181 ggtgtccgta acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca
     3241 gtgcaatagt aaatgaatgt taattacagt tatcttaatt gtttaaaaaa agtgattttt
     3301 atcacaaagg aaatatgcct gagtagcaat cagagacata actggcacgt aaggtttgca
     3361 accactaacc caccaataga gaggtagaaa gggcgttaat ctcccatact taacctggtt
     3421 tatggtaaat tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa
     3481 tatttggtca aaagaagaaa ctctgtggag tttcgcgctc tacggcacag ccgttggtgc
     3541 aggcacgctc ttccttccta ttcagttagg ttcggcgggg gctgtggtcc tgtttattac
     3601 tgctctggtc gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct
     3661 ctcatcgaaa acatcagcag gtgaagggat aacgggcgcg gtaacacact actatggcaa
     3721 gaagattggt aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat
     3781 atatgcagtg gcaattacca actcacttac agaacagctg gcaaagcata tggttattga
     3841 tcttcgcatc cgtatgttgg tgagtctggg ggttgtatta atcctgaatc tcatttttct
     3901 gatgggacgc catgccacta ttcgggtaat gggatttttg gtattcccat tgattgccta
     3961 tttcttattt ctttctattt acctggtcgg tagttggcaa cctgatctat taacaaccca
     4021 ggtagagttc aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt
     4081 cgcttttagc catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg
     4141 cgaacacgct atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat
     4201 aagtgtattg ttctttgtct ttagctgcct gctttctatt ccaccttcgt atattgaagc
     4261 ggctaaagaa gaaggggtta ccattttatc ggcgctttct atgctgccga acgccccagc
     4321 atggttgtca atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg
     4381 tacgtacttt ggcgttattg aaggtgccac agaggtcgtc aaaacaacat tacagcaggt
     4441 tggtgtaaag aaaagtcgtg catttaaccg cgcactatca attatgttgg tatcgctgat
     4501 taccttcatt gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg
     4561 cccgctcatt gccatgatac ttttcatcat gcctacgctg tcaacgtatc tcatcccggc
     4621 gcttaaaccc tggcgttcca tcggaaatct gattaccctg atcgtgggta tcctgtgcgt
     4681 atcggtaatg ttctttagct aatatccgtt gtggatggtt caaaattgct tcaattttga
     4741 accatcccag ttttcataat gttcatttgt tattattccg ctctatc
//
LOCUS       DAZGTJ010000197         4766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000197 DAZGTJ010000000
VERSION     DAZGTJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.198"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHK8E_005062"
     CDS             complement(<1..266)
                     /locus_tag="ACHK8E_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0347175.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIV"
     gene            complement(418..1158)
                     /locus_tag="ACHK8E_005063"
     CDS             complement(418..1158)
                     /locus_tag="ACHK8E_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP0347176.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1382..1567
                     /locus_tag="ACHK8E_005064"
                     /pseudo
     CDS             1382..1567
                     /locus_tag="ACHK8E_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1796..4105
                     /locus_tag="ACHK8E_005065"
     CDS             1796..4105
                     /locus_tag="ACHK8E_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFP0347177.1"
                     /translation="MELWIFIVAIYLIYILFFKKKKTKSAGSYTSAPVKAPPKEWLAN
                     NKKNQSVMQNEDSEDDNLATFTLSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMI
                     TVSGIEVTGGHFYLGQRMKPAGHNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGY
                     WPSFSSLSPEARGAYLSWLASDRCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEF
                     YSLFKEICRLRSVFNENRSFRNYSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRL
                     ILAKTVDTGAPVPADLALTWVTNHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVV
                     KPNKARLRLDYTPASPSLRGIRLPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRY
                     LGRKGTSANDTAAILLLPSEIVNESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMN
                     ATCPAKINKKEADLMQAFAQKMGYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYP
                     EFITAVLTLRLGSVVALIDNSLDQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHT
                     PANMTGMKSRIELLGAAEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSV
                     SSNIHQHSATETALVSSVPANQSTAGFTLDANVLARHESATDDVRKLLNSIFTEEEPE
                     QPESAPASLTGEGGLDSAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDW
                     SYAVVDAPVLEDADDHIWIDLEIAKELEG"
     gene            4109..4510
                     /locus_tag="ACHK8E_005066"
                     /pseudo
     CDS             4109..4510
                     /locus_tag="ACHK8E_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(4481..4689)
                     /locus_tag="ACHK8E_005067"
                     /pseudo
     CDS             complement(4481..4689)
                     /locus_tag="ACHK8E_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1356 a         1082 c         1081 g         1247 t
ORIGIN      
        1 acaataaaac tgttgttgat tttaccgtac gtcatacccg caggcgcata cagtgcacaa
       61 cagaacagaa ataacagagc tgtaatggat gtaagggtat ttctgtgtgc aaggagcaga
      121 agaaggaaca gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt
      181 gtggttatga cagaaataac aaaaaagaat aaaaacaata aaatccgtgg tgtgttgtcc
      241 ggacaaaaca gttttctgat attcatcgga gtatatcgac aacattattg tgaagagaat
      301 aaaaatcaga agtctcctgt gaaacagata acaaacaacc ctgcagtata attactgcag
      361 ggagtcttcg tttaattaca gaagatgggt aattattatc tttcaggagc agttttgtca
      421 gaaattataa gtcacaccaa gcatgatgtc atgactttta acatcaactt ctgatttata
      481 tttatcgccc cactcgtcct tgtaactcac actgctgtca cctgcatcaa gatagcgata
      541 gctgaggtcc agagcgatat ccggggttac gtcatagcga acacccgcgc caaggctcca
      601 tgcgaagttg tcagcagagc ctgaacgtga caacgattcg cgaccactgc ttccgtaccc
      661 ataatcccag gtactgatac cggttgtttt ctggtgaatt ctggcgtagc caatccctgc
      721 agatacccat ggtgtgaatg cactgtcatt ccggaagtca tagtacgcat tcagcatcag
      781 tgtgttgact gacacctcat tcttcaggtc atcacgccag taaccgcctg accagctgtc
      841 tttatctacg ttatacttcg aatcagcttt tccacgagcg taaaactcca gttccgtacg
      901 aaccggaata ctgaactgcg ggtaaaaatc ataaccggcc gcgataccgc cactgaatac
      961 cgtatcatgg ccatcaccgc ctttatattt tgatgtttcc tctccatcac ccgacaggaa
     1021 acgctggtct gcaagtgaca taacagaagc acctgcttta ccggtcacat aaaagcctgt
     1081 tttgctctca tcagccagag cctgggtcga aaacatgcct gccatggcaa gcgctgaaac
     1141 cgcaataacc ttattcattt caatcatctc cgctatcact ttccccgaac gataaatggg
     1201 gttgctcatt ctaaaaccat gcaaaagaca gggtaaaagg aatgttttcg atcgaatcga
     1261 tcgataaaat cgatcgtgcc cgataaagtt ttcttatcgt ccccataaaa atggtaaggg
     1321 ctggtttaag aggtaatcgc agcggcatgg aagtattaag accaaaacag cacgagataa
     1381 agcggctgaa cgtcagatga gccacatgga acgtaattca ttaagggcgg cttatatcca
     1441 taaaacagaa cacctggaat agcgtcggtt gatgctacag tggtgggggg attttctgga
     1501 tacaaactcg aaagaaaata ccagcccctt tgagtatgcg aagattaaca atccattaaa
     1561 atattaagca ctccgggcaa tctgcccgga gtgctttagc tggcataacg ggctactgat
     1621 ctcctctggt tacagataac gacttcagcc ctctgaaaga gaaaagcgga agctcaggaa
     1681 ggttgtgatt tgcttttaac ctttttaatt tagaagtatt attttgagat taatccactt
     1741 cccatcgtca tcctttttgg tgtgcgtgaa aagctatttt taaggttact gcctcatgga
     1801 gttatggata tttatagtag ctatttattt gatttatatt ctttttttca aaaagaagaa
     1861 aactaagtcc gcgggctctt atacttccgc tcctgtgaaa gcaccaccta aagaatggct
     1921 tgctaacaac aaaaaaaatc agtctgtaat gcaaaacgag gacagcgaag atgacaatct
     1981 ggctacgttt acactaagtg gcgggcggaa tgtcgaatac aggataacag ccactcaacg
     2041 ccagaatttc tcaaaaacta ctggtgcttt ggctcgttgg atttttccgg gagagatgat
     2101 cacagtcagt gggatagagg ttaccggcgg tcacttctat cttgggcagc gtatgaaacc
     2161 tgccgggcat aactcaggcg gttattatga tggcaataac gaggcatcgc tagttgacga
     2221 cacgcttaaa atatacccca caccttacat ttacgaagat agttcattag ggtattggcc
     2281 cagtttttcc tcactttcac ctgaagctcg tggtgcgtac ctgagttggc ttgccagcga
     2341 cagatgcgac acttcctgcc caattggtta cgttttcatt tatctgtatg gtctggaaag
     2401 aagggcgctt gttgacagca gggacaacaa tatatccgac gatgagttct attctctttt
     2461 caaagagatc tgccgtctca gatcggtttt caatgaaaac cgatctttcc ggaattactc
     2521 atcccagtta cttgaagcga tgtccattct tcgtccgaac gaaaatcttg cagcagctct
     2581 caacgatgat tcggagttca gcaatagcat gcagttcagg ttaatcctgg caaagactgt
     2641 tgatacagga gccccggttc cagcggatct ggctctgacg tgggtgacaa accatgctga
     2701 atactcatta cgaacccccg cccgccgctg tgcgaaagaa tttgctgcac tatttaaacg
     2761 gcgttacacc cttaaatacg gtgaagggat ggtagtgaag ccgaataaag cgcgtctgcg
     2821 tttagattac acgcctgcaa gtcccagttt gcgaggtatt cggcttcctg ttcccgactt
     2881 gcctgatcca ggtgcattga aaggcccagt tcagaagctt atggccattg ccgatatatg
     2941 cacaggtgaa ctggatgctt acagtcgtta ccttggtaga aaaggtactt cagcaaatga
     3001 taccgctgca attttgttgc tgccttcaga aatcgttaac gaaagcgcag aaaaaatatt
     3061 aagtacattc aaacactggg ctgatgacgc aatccgcagt aagggtggga tagtttcagt
     3121 tgctgactac tggtcacata tgaatgccac ctgtccggcg aagatcaata aaaaagaggc
     3181 cgacttaatg caagcctttg cacagaaaat gggatactgt ctggctcctg acccctatca
     3241 ccaccacgta aaggcagatg tcgatggtgt acttgtctta ttccctgctg gtgaaagagg
     3301 acgtttctca ccttatcctg aatttatcac agctgtattg acgctccgat taggttcggt
     3361 agtcgccttg attgacaatt ctctcgatca agccgaacag aaagtgcttg agaatgccat
     3421 caacaacaat gccagcttca gcgatgatga aaagcgctct ctgcacgcat atttaacgtg
     3481 gcagcttcat actccggcga atatgacggg aatgaaaagc cggattgaat tactcggtgc
     3541 agcggaaaaa tcagctgtgg gtaaggttat cgttagtgtc gcttgctctg acggcagaat
     3601 tacgcctgcc gaaatcaaac agcttgagaa gatttataca agcctgggac tggatccctc
     3661 atccgtatca agcaatatcc atcagcattc cgcaactgaa actgctctcg tttcatctgt
     3721 accagctaat cagtcgacgg cagggtttac gctggatgcc aacgtacttg cccgccacga
     3781 atctgccact gatgatgttc gtaaattact gaactcaatt ttcactgaag aagaaccaga
     3841 acaaccagaa agcgctccag cctcattaac cggggaaggg ggacttgact ccgctcatag
     3901 ccaactttat cgcaggttgc tggagaaaga acaatggtcc agaaaagagg cgacagagtt
     3961 gtgtagtaac tggaacctga tgctcggcgg tgcgcttgag gtgattaacg attggtccta
     4021 tgcggtggtt gatgcaccag tgctggaaga tgctgatgat cacatttgga ttgacctgga
     4081 aatagccaaa gaattagagg gttagtgaat gtctgtaaca cgtataagag tgaaagagcg
     4141 cgacgctatc attcagtcac ttaagtcggg agtaacgcca agaataggta ttcagcatat
     4201 ccaggttggc cgcgtgaatg agataaccgc cctccatcag gatattgaac ggattgctga
     4261 tggcggggca agcttcagac ttatcattgg tgagtacggt tccgggaaaa cattcttttt
     4321 aagtgttgta cggtctattg cgttagagaa gaagctggtc acggtcagtg cagacctttc
     4381 tcccgacaga cgtatccact catcgggagg tcaggctcgt aatctctact cagagcttat
     4441 gaaaaatatg tccaccagaa acaaaccaga tggcaatgca ttacttagcg ttgtggaaag
     4501 gtttgttaca gtcaaggcag tcaccaaact cgataataaa gcggctcatt tccatacgtc
     4561 tgtccttaag gggcattgtt catttctgtg accaagccgc accacctttt ttactgattc
     4621 gtctgtcggg aacactttgc gtttcttgat gacataacgg atcacactat tcaacgactc
     4681 tatggcattt tgcacatact tccatagcaa ctgtcatcgg tagctactga accagcaagt
     4741 taagtaagtt actacgtgaa aatttt
//
LOCUS       DAZGTJ010000198         4763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000198 DAZGTJ010000000
VERSION     DAZGTJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.199"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(179..1216)
                     /locus_tag="ACHK8E_005068"
     CDS             complement(179..1216)
                     /locus_tag="ACHK8E_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0347178.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(1280..2764)
                     /locus_tag="ACHK8E_005069"
     CDS             complement(1280..2764)
                     /locus_tag="ACHK8E_005069"
                     /inference="COORDINATES: protein motif:HMM:NF019153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFP0347179.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESRIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     RPVFIVTQNIPDVTKWFRDIWLRAGFCTGILRFPETARLLFSGE"
     gene            complement(2757..4247)
                     /locus_tag="ACHK8E_005070"
     CDS             complement(2757..4247)
                     /locus_tag="ACHK8E_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP0347180.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGYRGTGYDTSGLVQENGHA"
     gene            4479..>4763
                     /locus_tag="ACHK8E_005071"
     CDS             4479..>4763
                     /locus_tag="ACHK8E_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347181.1"
                     /translation="MSEIKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIR"
BASE COUNT         1167 a         1423 c         1013 g         1160 t
ORIGIN      
        1 gttaagggat gagatccttg acgggcggtt aatacaggat gcggcatcaa agggtctccg
       61 tttgtgtaag ttgtggttac actgattgag ttcggtatcg tatcctcaaa cagggtgtct
      121 tttgcagaga cgccctgttc tcgttaacac tgtatgctta cattgctgat tcagcctgtc
      181 aagatttatt cccccctttg caaatcttgc tcactgtgcg ctcatctgta tacaaactca
      241 gggcatcttc cagtgtctgc gctgcagact ccatacagac atcaacatac cccatggtcg
      301 ttgtaagact cctgtgtccc agtatggatt tgaccagatg taaattacgt tcaggatatt
      361 tcatcagtct ggttgccagc gtatgtctga aacgatgtgg tgaaacagga aaaccacatt
      421 ctcgcgaaag tcgccggaaa aatgaacgta cggtctgatg cgcccgaacg ggacagtact
      481 gatatctgtc ggcagagatc ggctcactga aacgacagac gtcaaaaaga tagtcatcat
      541 cacctgcccc gcactgacgg gcccgctcca gcagtttttc catcgatacc ttcaggggag
      601 ccaccagagg aacgccccat tccctgtgag ttttacttcc ctccagctta agatcaatta
      661 tgctttcatc cggacagaca tccctcacac ggatatgctt cagttgatta aagcgcattc
      721 cggtataacg cagaacatcc agaactgtca gccagtacca ggcagggtat atagcattgc
      781 gtcgacgact attcaccatg ttctgctctt ccgcttctgc aaacctctgc acaaccaggt
      841 aaaccctgag catctgattg tcactcagtg tctttttctt tttttcagta ctcgcaactg
      901 atgcttccac caacgggttt tctgtctgag gcagcagttt catcttgatc ccgaatccca
      961 taattgcccg caagtgggcc accttgttat tccaggtcac ctccctgcgc ttctgctctt
     1021 tcaggacata acgccggtat cgctgaacat cacggacagt cacctccccc ggcaaaatat
     1081 caccgataaa ttccctgaat accctgacaa ctttgcagta gctccactct gtatccggcc
     1141 gaagattctt tgaaaaaaag tactcttcca gcagttgttc ccaggtgatt tttgtgacgg
     1201 aatcgtcatt ctgcattata tattcccctt gtttactgtg ctcccgcact ctccggtcaa
     1261 attctgcaca tcatcgcctt tactcacccg aaaacaataa gcgggctgtc tccgggaacc
     1321 gtaatattcc cgtacagaaa cctgccctta accagatatc ccgaaaccat tttgtaacgt
     1381 ccggaatgtt ctgcgtcact ataaagacgg gcctgaaaaa acgctctcct gaacgtacct
     1441 tatggatacc tgcccgttca aagagcgact gaacataacg acgatcctgc ccggaaccac
     1501 tctccttcag aaaacgataa aaaatccccg gggcacacag aaaaacatat ccggaaacaa
     1561 catgaacatt tgaatcaacg ctgttaaccg aaagtgaacc ctccctcagc ccctctttta
     1621 accatctgat aaattcactc tctaccctgc tatcctctgt cagggaaaac tctgccgttt
     1681 tttccactgt ttccgggata cggtttccct gattttctgt tacacatccg ctctcttccg
     1741 gtaactcaat acggctttct ttatccggcg gcacgcctga cgctatctgc cctccctcat
     1801 ccgacaaatc agcagccgaa aacagcgtaa ggagcattcc ggtatcagca tcagtttcgt
     1861 tcccctcatc cacctcctgt tccgctgtcc ggccctcatt ttcggcatcc tccactggcg
     1921 actgtacaac cggcaacggc tgaatgacgc tttctgctga aattgctgct gactgcagca
     1981 gtaccggaac aacctcagcc accggaactt cggccacatc tcctgcagcg aggggactca
     2041 cgacttcctc catagcttcc tgtaatactg tacgtatcag cgccatttca ggatgctgat
     2101 gccagactgc tccggcaaga ttctgtaatg cccccgggac agtggcaagc caggctcccg
     2161 cctctccggg cagcagttgt cccgccatca gggctgccag ttgacgtccc tccgttccct
     2221 ggcagggcat cttcctgaac cggaaccggt atacgccctc cggtacagat atccccggct
     2281 gccagcatct gccgtcggcc tgttcgcctt ccagttccgc cagcaacggc aggtgataaa
     2341 acagagccga ccagaacacc accatgttcc acacactccc ctgcgcggcc tgctcttctg
     2401 ctgtcgctcc cggtggaaac attctgcctc tggccagccg gacagcatac gtcgtgaact
     2461 gcagggtcag gtcaccaaaa ccaccggtgt aaccccagtg accttctgct gtggccggca
     2521 gattctgact gacagacaac aactgatgca caggatcaag acaaaggcgc tgatacatat
     2581 ccaccggcat ggaactgttt tcccataact gtttcagaca ccttttcctg aacggtgtgg
     2641 ataccagtac atcagccggc aatggtgtaa aatacccctc cggcaccgtt tccccgggaa
     2701 tatccgtccg gacatcagag cattctgtct tcacaggaaa taaccttttc aggcgttcaa
     2761 gcatgcccat tctcctgaac cagacctgaa gtgtcgtacc cggtcccccg gtaaccttca
     2821 gttttctgct gttcttctgc cagttggtgc atcaggtcat tcagtaccag cgtcctgagg
     2881 gcccgggtga cagccacata taacagacag gtttcatcat gacgctcggc ctccgtcatc
     2941 agcggatccg tgatatcctg gaaatcctca tccagcatta caaccggcca ctccagcccc
     3001 ttgctccggt gtgccgttga taccgtgata tctgcatcct gctcattcgt taccgtatac
     3061 tgccggagaa gttccagttt tttcggtaac ggaaaacaca actccagaag ccgaagggac
     3121 tgattcatct ccacatcctt cgtggatttt gcaatatatt tatattcttc aaaattcctg
     3181 tagtcccggc gcagcctgtc tgaatgcatc cgttccggca tatcaacctg gaaccagtag
     3241 agatcctcaa gttccccggt cctgtaacct tcgattcccc ccacccagta aaccttttgc
     3301 cctttcattg ccgccatcag cgcagtacca atcacacccg cgacagtacg actgagtacc
     3361 gtgtaatgcc cctgcagatg ttcacgcgca tggcatttca gcaatacctc atcatcacca
     3421 ccacaacctt tcacctctcg tgtttcacct tcacgctgca gaagcagatt tgccatacga
     3481 gccaccgccg gaccaaaacg gaaactctgt gtcagccaca accggtccgc attcttcagt
     3541 gcaggatggg aaagcgcatt attcgcaccc cgaaagcggt aaatctgttg ataacaatct
     3601 ccaacaagga ccacccgaca cttctgcccc agaaccagag cagtggtcac cggattcgca
     3661 tcctgcgcct catcaaacag gatggtgtcc catcggtgag acaggtctgc accggacaac
     3721 tggaagagtt tcagataagt atcgtgcgtc accgggaacg tccccccggg gttgctcatt
     3781 tcgttccaga gcaactgcac cgcccgaagc gcatctgctg gcggaacacc ccgcatctca
     3841 tccttatccg gcaggtgatg caggcccggt accgcatcgg cagaacagat aaagtgattc
     3901 agccccgtcc ctgcaacccg tgccagagcc cagtatcgtg tattcagctt tctcgccaca
     3961 tcagtaatac gtaatcccgg aaccagccga tgacggtagt gtcttccaac ccgggcatac
     4021 gccagctgat gtgacgttct gcattccacg ttaaagggaa atttacgctc tgcctcatca
     4081 cgtattgccc ggttataggc aagatagagc atacggctgt ccggactggc ccgggcaaac
     4141 tgcaccagcg tactcgtctt ccctgtgccg gaaaacgcat tcacaaccag ttgctgcccc
     4201 tgccagttaa taatggcgta ctgttcatct gtgggattaa aagacatgca ttctcttctc
     4261 cggaaaaaca tttccgtaat ctgatgcatc aggggtatgc ccaccacaaa taactctgta
     4321 cagcaacaga aaaatttccc tggtcatatg atgaattaat catcgccatc acaccggatg
     4381 cttctcatcc tgtcctcaga aaggcaggtg gtctcctgtc tgccgcgctt atccaggata
     4441 ccgggacctg aaaggtcata atctggcagg aaaaaataat gtccgaaata aaaaaacacg
     4501 tttcttattc attaacagtt gcagctatgc tgtcagccgc catcgtctgt cagacagcac
     4561 atgctgatgt caacacaccg aacatcaccg gagaaaaaaa caatacaaca acacgattct
     4621 ccggaaatat ttacggcagt gaaaatactg tcacatcaga gtcaaacagt ctggttgtgg
     4681 gtaacagtaa ttccgtggag ggagggtata acaacattgt tattggtaat tcctccacgg
     4741 tcaaagcttc cggtttgatc aga
//
LOCUS       DAZGTJ010000199         4596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000199 DAZGTJ010000000
VERSION     DAZGTJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.200"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHK8E_005072"
     CDS             complement(<1..593)
                     /locus_tag="ACHK8E_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0347182.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHK8E_005073"
     tRNA            complement(708..783)
                     /locus_tag="ACHK8E_005073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHK8E_005074"
     tRNA            complement(790..864)
                     /locus_tag="ACHK8E_005074"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHK8E_005075"
     tRNA            complement(981..1065)
                     /locus_tag="ACHK8E_005075"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHK8E_005076"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHK8E_005076"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHK8E_005077"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHK8E_005077"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP0347183.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHK8E_005078"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHK8E_005078"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP0347184.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHK8E_005079"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHK8E_005079"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP0347185.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1181 a         1154 c         1044 g         1217 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactg
//
LOCUS       DAZGTJ010000200         4488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000200 DAZGTJ010000000
VERSION     DAZGTJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.201"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..546
                     /locus_tag="ACHK8E_005080"
     CDS             <1..546
                     /locus_tag="ACHK8E_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0347186.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..2886
                     /locus_tag="ACHK8E_005081"
     CDS             562..2886
                     /locus_tag="ACHK8E_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347187.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGSNKRKYGLVTL
                     KGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAMVFDKTVSLSS
                     PFAIKVGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDAFTGTFTKDARVTTRTGILPLKQVQTEVDKSTKQEYWQLRPENAY
                     VPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDYLDGKKQPTGFFRSLINSLYEAANDD
                     TRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFK
                     KDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPLLWHMHPIMFLGCM
                     KKSSGIWPLGKTSERYESGGRGPGVISSGKGDHGGVSYGMYQLSSKMGTVNKYIIMSK
                     YKDLFEGKKPATPEFNEIWKKIALIHKEEFAQDQHLFIKKTHYQVQLDYLASKGLNFS
                     HSLAAINDMIWSTSVQYGPTTGLISRALKGKNLETLTDADVITIVQDYKINHVEDLFP
                     SSPTWWDDLKTRALTEKKDLLELNELNLELKNED"
     gene            2876..3232
                     /locus_tag="ACHK8E_005082"
     CDS             2876..3232
                     /locus_tag="ACHK8E_005082"
                     /inference="COORDINATES: protein motif:HMM:NF045244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347188.1"
                     /translation="MKIKLFALLIFLPIISFAKEPHVCNSSMQSHPDIIDCIQISTRK
                     IEDSIKEIIAKSGNEYSIDMAFYNSQRKSISERCSLYYSMEGQRIDLIENQCEYDGVE
                     QLNKFIKTYMKTVDNY"
     gene            4033..4290
                     /locus_tag="ACHK8E_005083"
     CDS             4033..4290
                     /locus_tag="ACHK8E_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0347189.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            4293..>4488
                     /locus_tag="ACHK8E_005084"
     CDS             4293..>4488
                     /locus_tag="ACHK8E_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347190.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGL"
BASE COUNT         1374 a          868 c         1059 g         1187 t
ORIGIN      
        1 attaggtttg tgaccgacaa aatcagggaa cttcagtctg cggtcctgct gggtagtgcg
       61 ccgcaggggg tggccctgac ctcaggtgaa caccttcagc tctgcagtac ccgtaacacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caaccaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tctctgaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaactctgac gctgaacggt
      361 ggtgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg tagatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt ggggggagaa
      661 tctaccgggc tgtacatgat aggtcgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccggttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgatt gcatgggaat ccggtccgct gaatttttcc
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagtggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtgga acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaacagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cgccgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctcagttctc catttgccat taaggtcggt gacccgattg ggcacatggg ctactatcag
     1441 gccccaaagg atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg
     1501 gatgataatc tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc
     1561 tggctgaagt acgccccggg gcttgcgttg tacaagaaag atgcttttac aggaacattc
     1621 acgaaggacg cgagagtgac gaccaggacg ggaattctgc cactgaaaca ggtgcaaaca
     1681 gaagtcgata agtcgaccaa acaggaatac tggcaattgc ggccagaaaa cgcgtacgtg
     1741 ccaaaaggtc aggtggaacc tcaacttttg tcacagtatg atctggcaaa gctggggttc
     1801 agaactgaaa tcgcagagcc taccagcttt gattacctgg atggaaaaaa acagcccact
     1861 ggattcttcc gcagtcttat caactcactc tatgaggctg cgaacgatga tacgcgaacc
     1921 agtcatgcgc tggtgaaaca taattatcag cgcctgctgg ataaaattga tagtggaagc
     1981 gaccgttact ctccaatgga atactggaga gcacttcata accccgacta tcgtgatgtt
     2041 cttcagaaaa caatcgttaa acatcccagc gactggtact tcaaaaaaga cgatgcaatc
     2101 tggcaaccat ttctgaatgc gctgaaaaaa gatgctccgg aatggaagaa atacagcgaa
     2161 gattttcttg ataagatggc ctggatgcag gatgtgacca cggagaaact gggccctttg
     2221 ttgtggcata tgcatccgat tatgttctta ggctgtatga aaaaatcgtc aggaatatgg
     2281 ccacttggga aaacttcaga gcgttatgag tctggaggaa gagggccagg tgtaatttca
     2341 tctggaaaag gagatcatgg cggtgtgtca tatggaatgt atcaattatc ttctaagatg
     2401 ggaacggtta ataaatatat tataatgtca aaatacaaag atttatttga gggtaaaaaa
     2461 cctgctacac ctgaattcaa tgaaatatgg aaaaaaattg ctctcattca caaagaagaa
     2521 ttcgcacaag atcagcatct ttttataaaa aaaacacact atcaagtaca attagactat
     2581 ttggcatcca aagggctaaa tttctctcat tctcttgccg caatcaatga tatgatttgg
     2641 tcgacttccg ttcaatatgg ccctacgact ggtttaattt cgagagcgtt gaaaggaaag
     2701 aatttagaaa cattaacaga tgcagatgtg ataactattg ttcaggacta caaaataaat
     2761 catgttgaag atctttttcc atcatccccg acttggtggg atgatttaaa gacacgagcg
     2821 ctaacagaaa agaaagattt attggaatta aatgaattga atctggagtt aaagaatgaa
     2881 gattaaatta tttgcattgc ttattttctt gccgattatc tcttttgcga aagaaccaca
     2941 cgtttgcaat tcctcgatgc agtctcatcc agatattatc gattgcatac aaattagcac
     3001 aaggaaaata gaggattcca ttaaagaaat aattgcgaaa tctggcaacg agtattcaat
     3061 tgatatggcg ttttataata gccagagaaa gtctatttct gaacgatgca gcttatatta
     3121 ttcgatggaa ggtcaacgaa ttgatttgat agaaaatcaa tgcgaatatg atggtgtaga
     3181 acaactaaat aaatttatta aaacctatat gaagacggtt gataattatt aaaatcaaac
     3241 tgtctttggt tatgttaatt gttaatggta ttaacaatat atgctcaatg ctctcaaaga
     3301 aaaatattga cagttctaat cctcaatttt ctgactactt ggtttccgta attaacaggt
     3361 catttagaag tatctcaata agcaagagtt ataggtctgt atgtgaagtg gccttattta
     3421 attttaatta tagtaatggt gtaaaattaa taggattgct ggacggggtt aataattaat
     3481 gactgtctca taaatgttca tcataacgag aaaacggcag tagtataatt cggatgcaac
     3541 atgcatctgg agacgttaag ggttgagtta atagatttga ctctaaatcc ttgataaata
     3601 ttaacttaga ctaaacaaat gggcttgaaa aaacattgct attgctctgc actttatttg
     3661 catttctcat atgtgataat tttgctgtta caaatgaaat gccaagggaa aatattccct
     3721 taggaaaaat gtttgtggta taaaaattaa tgacgaactt gtagagatgt agatagttct
     3781 aaatgtgaat tttattgcgc actatctgta gtatcgcatt catatttgta tgaaattggc
     3841 aaaaaataat agtatttatt gataactcaa tggtaaatac atgcgatggg gaacactatc
     3901 tatcaataat cagtgacccg gaaacattca taataaaaat ccagacaatc actatcgatg
     3961 gcagagccca gcataaacat gggaattaaa gagtgatgaa ttagttaaga gataagaaag
     4021 aggaatttaa taatgcttca tattattcgt aaaggcgata aaactactca cggaggttcc
     4081 gttctgaccg cttctgaaac gatgaaattc ggtgggatag gcgtcgcccg taaaggcgat
     4141 aaagtgtcat gtccgggtca tggtcctacc atcatcatag aaggcaatcc tgattatctt
     4201 gaccatggta tcccggtggc atttcacggt cataaatgcg cctgtggttg cacattgatc
     4261 agttcatttg ccgcagcaaa ggttgcctga ttatgcctgt ctggctggat gccatccctg
     4321 aaaaggcccc aaagatagcg cgccccaata ctcgccgctg gctgttgttc ctggcattta
     4381 tcatgctggg ggggatggcg ctcacattat ggtgctggac gtcagaacga acaggatttg
     4441 tcttctggtt caccgctctg gggcttccat tttgcacgtg ggggctta
//
LOCUS       DAZGTJ010000201         4432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000201 DAZGTJ010000000
VERSION     DAZGTJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.202"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..853
                     /gene="ompT"
                     /locus_tag="ACHK8E_005085"
     CDS             <1..853
                     /gene="ompT"
                     /locus_tag="ACHK8E_005085"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0347191.1"
                     /translation="DISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINW
                     DLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNI
                     KGWFLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQ
                     RFKMPYIGLTGSYRYEDFELGGTFKYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYS
                     VAVNAGYYVTPNAKVYIEGAWNRVTNKKGDTSLYDHNDNTSDYSKNGAGIENYNFITT
                     AGLKYTF"
     gene            1085..1753
                     /locus_tag="ACHK8E_005086"
     CDS             1085..1753
                     /locus_tag="ACHK8E_005086"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0347192.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1835..2177)
                     /locus_tag="ACHK8E_005087"
                     /pseudo
     CDS             complement(1835..2177)
                     /locus_tag="ACHK8E_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2190..2785
                     /locus_tag="ACHK8E_005088"
                     /pseudo
     CDS             2190..2785
                     /locus_tag="ACHK8E_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3269..4222
                     /gene="ompT"
                     /locus_tag="ACHK8E_005089"
     CDS             3269..4222
                     /gene="ompT"
                     /locus_tag="ACHK8E_005089"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0347193.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT         1415 a          960 c          937 g         1120 t
ORIGIN      
        1 ggacattagt cttggaactc tgagcggaaa aacaaaagag cgtgtttatc tagccgaaga
       61 aggaggccga aaagtcagtc aacttgactg gaaattcaat aacgctgcaa ttattaaagg
      121 tgcaattaat tgggatttga tgccccagat atctatcggg gctgctggct ggacaacact
      181 cggcagccga ggtggcaata tggtcgatca ggactggatg gattccagta accccggaac
      241 ctggacggat gaaagtagac accctgatac acaactcaat tatgccaacg aatttgatct
      301 gaatatcaaa ggctggttcc tcaacgaacc caattaccgc ctgggactca tggccggata
      361 tcaggaaagc cgttatagct ttacagccag aggtggttcc tatatctaca gttctgagga
      421 gggattcaga gatgatatcg gctccttccc gaatggagaa agagcaatcg gctacaaaca
      481 acgttttaaa atgccctaca ttggcttgac tggaagttat cgttatgaag attttgagct
      541 aggtggcaca tttaaataca gcggctgggt ggaagcatct gataacgatg aacactatga
      601 cccaggaaaa agaatcactt atcgcagtaa agtcaaagac caaaattact attctgttgc
      661 agtcaatgca ggttattacg taacgcctaa tgcaaaagtt tatattgaag gcgcatggaa
      721 tcgggttacg aataaaaaag gtgatacttc actttatgat cacaatgata acacttctga
      781 ctacagcaaa aatggtgcag gcatagaaaa ctataacttc atcactactg ctggtcttaa
      841 gtacaccttt taacaacgtt aaatagattt tctccccggt ggtattgtat agccccgggg
      901 aatttgttta ccgttaaaag caaacaatat cccctaatct tgtaagcatt taacctgcgt
      961 cctggcgtgt aacctcttac cgcaacacaa gactcaatat attcaaattc gttatgccgt
     1021 ggcatcattt aaccccttgt aattcatcgc tataattgat ttaattcaca aataaaacta
     1081 taacatggtg aaactaatga aaaaaaacac agatgatggg gctaaaattt acacaccact
     1141 taccctaaag ctttatgact ggtgggtttt gggtgtatca aatcggcttg catggggatg
     1201 tcctacaaag gaacaccttc ttccacactt tctggaacat ttaggtaaca accatctgga
     1261 tattggcgtt ggaactgggt tttaccttac tcacgtacct gagagtagtc tgatatcttt
     1321 aatggatttg aacgaagcta gcctgaacgc ggcatcgaca agggctgggg aatcaaaaat
     1381 taaacataaa attagccatg atgtttttga tccttatccc gcggcgttac atggtcaatt
     1441 tgattccatt tccatgtttt accttcttca ctgcctgcct ggaaatatat ctacaaaaag
     1501 ctgtgtaata cgcaatgcgg cgcaggcctt aactgacgat ggaactctat acggagccac
     1561 aattcttggt gacggcgttg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa
     1621 tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc
     1681 agagcatttc gagaatgtta aaaccaaggt taaaggtact gtagtaatgt tttccgcttc
     1741 agggaaaaaa tagcatccag ccgcagcacg ttcttgcgca cgacgttctg cggcataatc
     1801 ccaatgatta ctctctgaca gggttcacag gccactaaat atcaggggca gttgatgtat
     1861 caactcggtt cagcaacacc cgatacatct tccaggcttc cagcaacgag gtttcttcct
     1921 ccgtatcctt tatccatgat atgccgttcc acttctgaaa ctcccctgcc ggggatacct
     1981 ggtgacattt tccggtaacg gaccgagttc agaaataaat aatgcgtccc ctgatgccac
     2041 gtcataaacc gttttacccc gatggtcttc aacgagatgc cacgatgcct catcactgtt
     2101 gaaaacaacc acgaagccag ccggaatatc tgggcaatat cagtactgtt tgcaggcaaa
     2161 cctgtatgag gtggaatggc acaagagact tacaaaagaa tcaggccaaa cgcctgattg
     2221 atgagagccc cgaaccactg aaatctgtcg tcaaatttgc gctggtaact gttctgagaa
     2281 agtcgaacat cataaatctg gagtggcaac agattgacat gcagcgacaa gttgccaggg
     2341 tgaatccaga agatagcaaa tcaaactgcg ccattggtgt ggcgctgaac gataccgcct
     2401 ctaaagtgtt gcgtgatcaa acaggcaagc atcacaaatg tgggtgtaca taccagggcg
     2461 gctaagcgag cagatggaac atcaatgcct gcgctcagga agatgcgcat tgacagcaag
     2521 acagcatggc tatcagcttg tcgtcgtgca ggaattgaaa atttccgttt ccatgacctc
     2581 tggcatacct gggcaagctg gttgattccg tcaggcgtcc cattatcagt gcttcaggaa
     2641 atgggcggat gggagtccag agaaatggtt cgtagatatg ctcaccttgc gcctaatcat
     2701 ttgactgagc acgcgaggaa aatagacgac atttttggtg ataatgtccc actctggaat
     2761 tatagaagaa ataaagaagg cgtaactgat tgaattttaa tggcgcgccc tgcaggattc
     2821 gaacctgcgg cccacgactt agaagttcct agaacgacat tttaagtcaa caacttaccg
     2881 cgccatctct gcgctcacac gtcccactac ctcaaaacat gtaaagcctt gcaagccatt
     2941 gcgaggcctt atgtgtctca gttttgtccc tcttttttgt actaaaaaac atagtaattg
     3001 aggataaacc tcatgctatt ttcgcttata tgcctctaaa ggcatggcac ttaaatagat
     3061 aaaagcacca caaaagcata aaaaaaccac acagtaaaac cgcaatatga aacaataaca
     3121 gataattaaa ccaaaaacag atagcgcatt gtgataatca ttcaatacta aacaaaatat
     3181 aaacagtgga gcaatatgta attgactcat taagttagat ataaaaaata catattcaat
     3241 cattaaaacg attgaatgga gaacttttat gcgggcgaaa cttctgggaa tagtcctgac
     3301 aacccctatt gcgatcagct cttttgcttc taccgagact ttatcgttta ctcctgacaa
     3361 cataaatgcg gacattagtc ttggaactct gagcggaaaa acaaaagagc gtgtttatct
     3421 agccgaagaa ggaggccgaa aagtcagtca actcgactgg aaattcaata gcgctgcaat
     3481 tattaaaggt gcaattaatt gggatttgat gccccagata tctatcgggg ctgctggctg
     3541 gacaactctc ggcagccgag gtggcaatat ggtcgatcag gactggatgg attccagtaa
     3601 ccccggaacc tggacggatg aaagtagaca ccctgataca caactcaatt atgccaacga
     3661 atttgatctg aatatcaaag gctggctcct caacgaaccc aattaccgcc tgggactcat
     3721 ggccggatat caggaaagcc gttatagctt tacagccaga ggtggttcct atatctacag
     3781 ttctgaggag ggattcagag atgatatcgg ctccttcccg aatggagaaa gggcaatcgg
     3841 ctacaaacaa cgttttaaaa tgccctacat tggcttgact ggaagttatc gttatgaaga
     3901 ttttgaactc ggtggcacat ttaaatacag cggctgggtg gaatcatctg ataacgatga
     3961 acactatgac ccgggaaaaa gaatcactta tcgcagtaag gtcaaagacc aaaattacta
     4021 ttctgttgca gtcaatgcag gttattacgt cacacctaac gcaaaagttt atgttgaagg
     4081 cgcatggaat cgggttacga ataaaaaagg taatacttca ctttatgatc acaataataa
     4141 cacttcagac tacagcaaaa atggagcagg tatagaaaac tataacttca tcactactgc
     4201 tggtcttaag tacacatttt aagaacgcca actaaaattt ccccgaggtg aaaatcgccc
     4261 cggggaataa ctagccattt caatgtaaca attaaccctt aaaataaacc cagaaggtta
     4321 ttaactaaat cacatagaaa accatcaatt atagtatgta taaaataggc gacagcaacc
     4381 caattacaaa ttaatggttc cagaatatca catcagaaaa aacgctgtat aa
//
LOCUS       DAZGTJ010000202         4265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000202 DAZGTJ010000000
VERSION     DAZGTJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.203"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(35..232)
                     /locus_tag="ACHK8E_005090"
     CDS             complement(35..232)
                     /locus_tag="ACHK8E_005090"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0347194.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            complement(480..905)
                     /locus_tag="ACHK8E_005091"
     CDS             complement(480..905)
                     /locus_tag="ACHK8E_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0347195.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(902..1285)
                     /locus_tag="ACHK8E_005092"
     CDS             complement(902..1285)
                     /locus_tag="ACHK8E_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0347196.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(1657..2250)
                     /locus_tag="ACHK8E_005093"
     CDS             complement(1657..2250)
                     /locus_tag="ACHK8E_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0347197.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            complement(3482..>4265)
                     /locus_tag="ACHK8E_005094"
     CDS             complement(3482..>4265)
                     /locus_tag="ACHK8E_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347198.1"
                     /translation="FEQLQNARGYVTRCYMLLAVFIYLIRRNKLHKALRFYDESQKYE
                     LPFGYLPGAFSVLRIALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNE
                     DEIQYDANNFTLMRVIKMYNSMLANISTKSDIQPPYITGLLDNVERALDKINILIDKE
                     RVYDGETLAELITENKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDYVISPAD
                     DIKNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
BASE COUNT         1287 a         1004 c          854 g         1120 t
ORIGIN      
        1 tatacataat aaatcacctt acagttacaa caggtcaaaa accgctgtag ccagagttac
       61 gctggcctga tgctttagta ccgggcttcg tcagataatc cagacgctcc aataagcgct
      121 gatactgctc agggaaatca ggatcatgaa tatccaggat gtaacgtcca ttagcaggga
      181 aatgaataat gcagccccct ggattaacaa tgcagaaatc gtcctgaggc actgatcaat
      241 acggagagga ctctcgcgtg tggtttattg acaccacagt gcagattcgg cgaatccgcg
      301 atcacggtgc aatttcgttc cacagcacac aatcataacc ccgggtttta ttcaggtaag
      361 caggactgcg gatatccggt gtcgcgcctt tgtgtcacga acggggtagg tgcgaaacac
      421 cggataaaat gcaggctggc aatacctctg aacgccctgc gcagagcgga tattttggat
      481 taagtactcg cacctccgca gtcctggcac aaatctggct ggtacctgta aacagacttc
      541 gtacatgttg ctctgcaata aatccccgta ccacaggcct cacagaattt cttcccatga
      601 aaaaactgct cacacatcac acaatgccac tccagcacga ccggtaatgg cgatagaaac
      661 atcgccatat cctcaatgta agggtgggac ttttccggat tcagcaccac gcaggtcgcc
      721 ttctgttgcg cgctcagggc atgtgaatca tgctcaaacc acgccccctg agcatctgtc
      781 tgcaaaatca accgaccacg acaggaaagg cagaaacact gccggatatt tctgctgagc
      841 gcagagtcgg cactgataat gtgttcaccc ggcgtgatcc ccagccccgt ttttataccg
      901 ttcattcagc cactccctcc tcactgaagt gccctgtatg gcagtgtgtg cagtaccgct
      961 ccccataata atcgtggtga cattgtctgc agcgccagct ggctttacgc accacgggta
     1021 aggcatccgg gacgaattgc tgcagacgtt taatcaaccg tatttctctg cgttccggcc
     1081 tgacataagg acattgctgc gcgtgctccg tcagcccttc gtctgcgtgt tcaaaccagg
     1141 gacgctcagt gtcgtattgc gggtggtatc tgagcgcact gccgcaaagg tggcaagtgt
     1201 agcgatcgta aggtgcagtc tgtgcggtac gggcacccgt cagacgcccg ttgccatcaa
     1261 atgcaataaa ggattttgcg tacatcgtca tattcctcag ccgccagacg acacgcaggc
     1321 aacagctgtc ccggtacggg cagtgtggtc agggtgtgaa tggcggttca gttaaaatct
     1381 ggactggagt ttgggtaaaa caggctggtt gctgttaccg cactgtggtt gtcacacaat
     1441 ggcccacctg cgtaaaaaac tgtcagcagg cagggatgga catcggggga tgtgctgtat
     1501 cggttgccgg aaggaatggt aaagaatgtg ccatggttaa atctccgtat ggttcatacg
     1561 gaatagccat gtcgtaaaaa atgcgcagag tccctgaagt ggcctccgga accgactgta
     1621 acaattagcg ccagcccgta gttgtcgttc aggggcttac accagagaac atatctccga
     1681 cagggtactg tccagtcgcg gtcggccccg gttacttttt tcgggaagcc tgctacgccc
     1741 cagaataata ccgtgttctt tctgatcctg tttagccagg tagctgaggg cgtaacgtaa
     1801 gtcgtctacc gcagatttat cactatagtg aatcacgtga tcgatacgca ccgggtattt
     1861 gtctttagcc cggcacagat gaaaataacc ctccccttcc gtgatccgcc tccagatatc
     1921 tcccagttgc ctggatatct gataagagtt cttgtgacgt tgtccatcca gatagccgat
     1981 aaaatgaata tgaagccctt tgtctggtgt atattccatt acccagtaat atcctgctaa
     2041 catcgtctgg gtttcgctaa gtaaacggta tatttccata cacatactgt gcttacagga
     2101 atgcccgaaa ctgggcgtgt ctttcctgta ggcaaaatca attctgaaag gtaacagttt
     2161 agaaaaacgt tgaaacatac cgtccatgtg ttcattcaca tctttcagaa tcgcgaaatt
     2221 catttcgtaa ttgggattgg cattatacat tttatagtcc ttactttata aaactcacag
     2281 gaagaggaaa ttatgtaaat gctgagggta cagaaacgtc gtactacggc attaataaaa
     2341 gagataacca gcagcacaga caacctgata aaactgtttt gtattacctc attacagcaa
     2401 tgaggtaata tttcagaata cagtgagact ctcttaaact gaggaccagt acaaattaca
     2461 atggtaaaaa caccacctca tagaattgag tttaagaaaa caccattcac tcaacctgac
     2521 attcacaaaa gtcagtacca ggtagcctca ggtaacatcg ctattaatat tatcctgcca
     2581 ttaagtaaaa acagaaactc tgtgaggaag cctcaccaat atttgaccag tagcataaag
     2641 aataacgata tgagagatac gcccacacca gagatgatta gcccatacaa aaagataata
     2701 tttattaata ccaccgaacc tcaatagagt aataacacac taatagcgtt ttagtacaca
     2761 cttaacacta actatgcacc agacacatac tcaggattaa cattctcaac atttatggta
     2821 ctctcaaaga gtataattca ccacacaggt aaactctcac accagtagta tggagataat
     2881 gctgtacaca tacttacaga gtaaagtgta cagctagcat acagtggcga gcagacatta
     2941 caacataaag actcaagata gcataataaa gcataataag aacacaacaa aactcccact
     3001 aataatacta attaacctta attctttttt gaacaggaaa tcaacatagc tccagacctc
     3061 ttcaggtcat acacttttgt attggatgtt caatgtgtac ttctgcacag acaaaaaaat
     3121 aaaacccaca aaataaaata tcaatcccaa taaaaaattt taaaatacag cgtgaaacac
     3181 cacgttatgg atatgttttc atatcaccag aagcatattt cacacataca atacaggaac
     3241 aattcatcat ataatcaggg ttactgttca ctcctcacgg ttatccgcga gacgataaaa
     3301 catgacacta atcacaaaaa cgagagatag tatttgcaaa atgcaaacaa gataaaaatc
     3361 aatgtgttgc gattttttat tttttcagac atgcatgccc cctctgctat tgttgctagc
     3421 cgactagcaa caatagcagg gatatacaaa catcggatgc caaaaacacg ttccgataat
     3481 atcatctagg gtctgactgg ttgtttttag tatcatcacc acaaatgatg gttaatgcgt
     3541 tgtagatcag tctgcgtctg ttcttgtttt cagcattatt tccatacaac atcatgacgt
     3601 ttttaatatc atcagccggt gatattacat aatcaaccaa tacgccaagc ttctcaatac
     3661 attgcagtaa agtatatttg tgtctgcccg taaataatcc tatcaggttt tctttacttt
     3721 cacgactgga taatattttg ttctctgtaa ttaattcagc caacgtttca ccatcgtaaa
     3781 cacgttcttt atctatcaga atatttatct tatctaacgc cctctctaca ttatcgagaa
     3841 gacctgtaat atacggaggc tgaatatctg actttgtact aatatttgca agcatcgaat
     3901 tatacatttt aatgacacgc atcagagtaa aattattcgc atcatattgt atttcatctt
     3961 cattatacgc aggatccgtc acgaccctta agtccataaa tgcatcctga taagctttta
     4021 cataatcaag taattcgaac agtgaaccat gctttatttt ctccctgttt actttgatct
     4081 ccagcgctat cctgagaact gaaaaagcgc cgggaagata cccaaaaggt aactcgtatt
     4141 tttgggattc atcataaaac cgaagagctt tgtgaagttt gttccgccga atcagataaa
     4201 taaaaacggc cagtaacatg tagcacctgg tcacataacc acgggcattc tgcaactgtt
     4261 caaaa
//
LOCUS       DAZGTJ010000203         4018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000203 DAZGTJ010000000
VERSION     DAZGTJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.204"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHK8E_005095"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHK8E_005095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP0347199.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHK8E_005096"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHK8E_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP0347200.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHK8E_005097"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHK8E_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP0347201.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHK8E_005098"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHK8E_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP0347202.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1051 a          980 c         1017 g          970 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag gggtgaaact ctgcggcctt tttcgttctt attgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggaga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcgatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacg cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaat gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcggtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gacaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gcggatgcga catcaacccg
     1021 gcggcttcaa tatccgacca ggaaagcgaa cagatattaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattta gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgaccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagaatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac gaataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa agaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca ttggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggtg cgattagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacagcggga gagtggcgca ttccacggga taaccatatt
     2581 tgcaccgcag gccaataata aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 tacgaggatt ttcatcgcac taccgcgctg gcgacgcaga gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ttccgcctcc tggtgttgaa ctaatgtcca gcaaccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaagca atatccagca aatgttggtc
     3001 gccctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gtaacaatga
     3061 aaattgttta tcgttattat ttccggaatt aattaccaaa agcgaacaat ggtttagccg
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata atgttatttt
     3301 tcccggccct tgagaatcat ctaattgaaa gattttcgct ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcaac
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagcaggtc acaagacatg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag cacattcccg
     3721 tctcgccgta aacaataaac cgacgaaatg ctcacagtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agaaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttttggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactg
//
LOCUS       DAZGTJ010000204         3992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000204 DAZGTJ010000000
VERSION     DAZGTJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.205"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            100..708
                     /locus_tag="ACHK8E_005099"
     CDS             100..708
                     /locus_tag="ACHK8E_005099"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP0347203.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            806..2197
                     /gene="selA"
                     /locus_tag="ACHK8E_005100"
     CDS             806..2197
                     /gene="selA"
                     /locus_tag="ACHK8E_005100"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP0347204.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2194..>3992
                     /gene="selB"
                     /locus_tag="ACHK8E_005101"
     CDS             2194..>3992
                     /gene="selB"
                     /locus_tag="ACHK8E_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP0347205.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
BASE COUNT          883 a         1020 c         1194 g          895 t
ORIGIN      
        1 atatgtgatt tattgggaag taacaaacca tcaacatttt ctgccctttt tcctatcacc
       61 aggccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt cggtagctac
      121 accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat aactttcgaa
      181 tttattaatg aactgcccta taacgcggat aacggcgtgg cgcaatttaa cccgttaggg
      241 aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc gatcatcgct
      301 gaatatattg aattaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg
      361 ttgcgggtgc gcaaaattga ggcactggcg gatggcatta tggatgccgg gctggtatcg
      421 gtgcgtgaac aggcgcgtcc agcggcgcag cagtctgaag atgaattgtt acgccagcgc
      481 gagaaaatca accgcagtct ggatgtgctg gaaggatatc tggtcgatgg cacgctcaaa
      541 accgatacgg tcaatctggc gactatcgcc attgcctgtg ccgtcggata tctcaatttc
      601 cgccgcgttg cgccaggctg gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac
      661 ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt
      721 cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc tccgtgtgag
      781 agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct atagtcaact
      841 tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg atacttatgg
      901 tcacacccgc gtggtggaat tgctgcgtca gatgcttgac gaagcgcgag aagtgattcg
      961 tgatagccag acgctgcccg catggtgtga aaactgggcg caagaagtcg atgcccggtt
     1021 gacgaaagag gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca
     1081 taccaatctt gggcgagctt tacaggcaga agccgcggtg gaagccgttg cgcaggctat
     1141 gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac atcgcgatcg
     1201 ggcgctggcg cagctgctgt gccgcattac gggagcggaa gatgcctgta tcgttaataa
     1261 caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcgggaaag aggtggtggt
     1321 atctcgcggc gaactggtgg agattggcgg cgcgtttcgt attcctgatg tcatgcgtca
     1381 ggcaggctgc accctacacg aagtggggac caccaaccgc acgcacgcta atgattatcg
     1441 tcaggcggtg aatgaaaata ccgcgctgtt gatgaaagta cataccagta actacagcat
     1501 tcagggattc accaaagcga tagatgaagc ggaactggtg gcgctcggca aagagctgga
     1561 tgttcccgta gtgactgatt taggcagtgg ctcgctggtt gatcttagcc agtacggttt
     1621 gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg tgagtttctc
     1681 cggtgacaag ttgttaggcg ggccgcaggc aggaattatt gttggtaaaa aagagatgat
     1741 cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc
     1801 ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg aaaaattacc
     1861 gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac aacgtttaca
     1921 ggctcccctt gccgcacatt acggcgcgga gtttgcagtc caggttatgc catgtctttc
     1981 gcagattggc agtggttcac tgccggttga tcgcctgccg agcgcggcat taacgtttac
     2041 accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc gtgaattgcc
     2101 agtgccggtg attggtcgta tttatgacgg acgattgtgg ctggatttac gctgccttga
     2161 agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc cggacacgtt
     2221 gaccacggca aaacaacctt attgcaggcg attactggcg taaatgccga tcgtctgccg
     2281 gaagaaaaaa agcgcggcat gactatcgat ctcggctatg cctactggcc gcagccggat
     2341 ggccgcgtgc ctggttttat cgacgttccc ggtcatgaaa agtttctttc caacatgctg
     2401 gcgggcgtcg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga cggtgtgatg
     2461 gcacagaccc gtgagcatct ggcgattttg cagctgactg gtaacccgat gctgacagtg
     2521 gcgctgacca aagccgatcg cgtggacgaa gcgcgtgttg atgaggttga acgccaggta
     2581 aaggaggtgc tgcgggaata cggttttgct gaggcaaaac tgtttatcac cgcagcaacc
     2641 gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttaccaga acgcgaacac
     2701 gccagccaac atagtttccg cctcgcgatc gaccgtgcat ttaccgtaaa aggtgccggg
     2761 ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc actctggctg
     2821 actggtgtaa ataaaccgat gcgcgtgcgt gcgctgcatg cgcaaaatca gccaacagaa
     2881 actgcccatg ccgggcagcg tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag
     2941 attaaccgtg gcgactggct gcttgccgat gcgccgccgg agccgttcac acgggtgatt
     3001 gtcgagcttc aaacccatac accgctgacc cagtggcagc cgctgcatat tcaccacgcc
     3061 gccagccacg tcacgggacg cgtttcgctg ctggaagata accttgccga gctggtgttc
     3121 gacacgccgc tgtggctggc ggataacgat cgcttagtgt tgcgcgatat ctcggcccgc
     3181 aacacgctgg cgggggcgcg tgtggtgatg cttaacccgc cgcgtcgcgg taagcgtaag
     3241 ccggaatatc tgcaatggct ggcgtctctt gcacgggcgc agagcgatgc cgatgcgtta
     3301 tctgttcatc tggaacgcgg agcggttaac cttgcggatt tcgcctgggc acggcagctc
     3361 aacggcgaag ggatgcgcga actgctgcaa cagcctggct atattcaggc tggttatagc
     3421 ttgttgaatg cgccggttgc cgctcgctgg cagcggaaaa ttctcgacac attagcgact
     3481 tatcatgagc aacatcgcga tgaacctggt cctgggcgag aacgtctgcg acgtatggcg
     3541 ttgccaatgg aagatgaagc gctggtactg ttgctgattg aaaagatgcg cgaaagcggc
     3601 gacatcctca gtcatcacgg ctggctgcat ctgccggatc acaaagcggg cttcagcgaa
     3661 gagcagcagg ccatctggca aaaagcagag ccactgtttg gtgacgaacc gtggtgggtg
     3721 cgagacctgg caaaagaaac gggaaccgac gagcaggcaa tgcgcctgac gctacgccag
     3781 gcggcgcaac aggggataat tactgcgatc gtcaaagatc gttattaccg taacgatcgg
     3841 attgtcgaat ttgccaatat gatccgcgat ctcgatcagg aatgtggttc aacctgcgcg
     3901 gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcaaat cctggaatat
     3961 tttgaccgca ttggctttac gcgtcgtcgt gg
//
LOCUS       DAZGTJ010000205         3989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000205 DAZGTJ010000000
VERSION     DAZGTJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.206"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..250
                     /locus_tag="ACHK8E_005102"
     CDS             <1..250
                     /locus_tag="ACHK8E_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347206.1"
                     /translation="IITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYH
                     EEQAHKHNAELTRTLASINRKAAIARYVHSAAGRQSRR"
     gene            299..469
                     /locus_tag="ACHK8E_005103"
     CDS             299..469
                     /locus_tag="ACHK8E_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347207.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            466..792
                     /locus_tag="ACHK8E_005104"
     CDS             466..792
                     /locus_tag="ACHK8E_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0347208.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTNKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(858..1121)
                     /locus_tag="ACHK8E_005105"
     CDS             complement(858..1121)
                     /locus_tag="ACHK8E_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0347209.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(1159..2211)
                     /locus_tag="ACHK8E_005106"
     CDS             complement(1159..2211)
                     /locus_tag="ACHK8E_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0347210.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVDAGHVAEEKAVPVG"
     gene            complement(2208..>3989)
                     /locus_tag="ACHK8E_005107"
     CDS             complement(2208..>3989)
                     /locus_tag="ACHK8E_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0347211.1"
                     /translation="KEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQWATLLPAE
                     SVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAEIAALRPAG
                     YEDGAAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRENRHRERRF
                     ILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFALQFGITLTG
                     KHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMATHDKYRTT
                     YFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEFPKESAMRVGCECPDGIWRYIIRLEE
                     AVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEVEMETWGDY
                     DPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQGFNFTWQA
                     DQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNRLVMKMIDV
                     VAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDVTGHADVFF
                     AISHAVINEPVDYDYERPSTWAFGKAA"
BASE COUNT          942 a         1181 c         1052 g          814 t
ORIGIN      
        1 catcatcacc gacatactca acggacgacc ggacagcacc aaccgcagct ggcttgtcgc
       61 cgttcgtccg cgccgtccgc ccggagagcc ggagccgctc acgcctgccg gaaaaatcat
      121 gcggcagtac cacgaagagc aggcacacaa acacaacgca gaactcacca gaacactggc
      181 atcgattaac cgcaaagcgg caattgcccg ctatgtgcat tcagccgctg gcagacaatc
      241 acgcaggtaa acaaagacca tgaacataac cagaaacagc caactaaagg agcgcaccat
      301 gaacagcacc aggaggatca gcgacagaga gcttacgaaa atgattgagt cggcacagca
      361 gctgctttca cgacaaaacc cggtcgtgga agaaacatgg tgggagaatt tactggcagc
      421 actgaccgaa ctccgggaac gccggaacaa cgaaggagcc gccaaatgaa gaccccagga
      481 atgaccggaa aggaaatcat tttacgctac ctggaaacgc acgcacgttt cagcgcccca
      541 gaactggtag aaaaactggg cgtgaaaaga accgtcatta atcaggccgc cggaaaaatg
      601 gtacgggaag ggttgctggt tgtggacggg tacacgaaca agataacgca ttaccggaag
      661 ccaacggaaa aggaacgaca ggaactggcg caacgggctg aacggcagca ggaaaaatca
      721 gtcatcgaag cctgcaagcg cagcgaaatc atgaagcgaa ttctgtttat ctacggcgct
      781 gcattccgtt aaaggagctg gtgagtcggg gcaaaaattc tttttgcccc ggcgaagcag
      841 cagacaagcg aagcgcgtca ggaacgccgc gacagtagtt caagcgcata ccggcgatct
      901 tccgggcgca tggcatccac caggaacttc accatattgc gaccggagcg ggcaccgggg
      961 ctgagggtat gggaaaaggt catcgtacag acgaaggtgt gcccgcattc gacatcactg
     1021 catgcacagt acagatccgc aagttcaggg gttttccagt ctgtcttccg gatgacagca
     1081 ggtgcgccac agtcaggaca ccgggcttta aaaatccgca tcttacctcc cgataacacg
     1141 tattccgggg aggacatttt acccgaccgg caccgctttt tcttccgcca catgcccggc
     1201 atccacatca aaacaaagat aaaggtgttc cggtatatcg ggatcgctgt tcacggcgtt
     1261 catgatttta cgctgaagcg gtatcacctc atccttgcgg tacgtttcac gcgtcgtttc
     1321 cgggttaggg atggtggccc cgttcgtggg gataatcccc gcaagaccgg caggaaagcg
     1381 gtgtgccgta aacacatcct gggcgctgat ccccttgatg tttgaaaatt catccttggc
     1441 gctcacctca ccaatcggaa taattttcac cccatccggc gaacctttgg ggatgttgat
     1501 gtacagattg cggaaatttc ccagcccctt agactccgcc accttctcgc ggatctcatc
     1561 ctccatctcc gtggtgatcc cagggtcgtt cgtatacagc aaaaagccca tatgcgcccc
     1621 gttgttgtag taacggcgac gaaaaaggct ggcctcgctg ttcagcagcg ccgagtggat
     1681 gccaccgata taatccggca gcccgtaaat ttgctgacgc ggatcataca tccgcagaaa
     1741 gataatatct tccggcgtat agaccagcgc gggaccttcc tgaagcacca cgaactcacc
     1801 ggttttgcgg cgccggagat acagcgacgg aagcggctca agtgccacga cgtcgccgaa
     1861 tacgttacgc accttgagca gggcgagatc gccgaacaac aggtagtcaa ataccgcctg
     1921 ttccatctgg tccgttgtga gaccaccacc ggtatagccc cccgccacca tgttacggcg
     1981 ggcatacagc accccaccgt gctgggcgtt aaggttcggg agctgcgcca gtgccaggcg
     2041 gttaatcggc agctgccagt ggttgtgttc gttgtcatac cagatgttat ggtagtccgt
     2101 gccggtggtc agcaccggtt ccggctgacc aaacgtgatt gtgctgccac gtgcgggcgt
     2161 gaatgctttt ttcgtcacgc gtgacgtttt gtttctgcgt ttcttgctca tgcggctttc
     2221 ccgaaggccc atgtggacgg gcgttcatag tcataatcta caggctcatt aatcacggcg
     2281 tgcgaaatgg caaaaaacac atccgcatgc cccgtaacat ccgaccgttc cgccacgaac
     2341 gtcagcgcgt taccgctttt tgtggtggtg cggcgtatgg acatgaacga cgcggggatc
     2401 tccgtgcgtt cacggctggc ctcatcaatg gcgtctttcg cccactcgat acgcttatgg
     2461 gccaccacgt ctatcatttt catcaccagg cggtttttgc tctccacgct gtaaagaatg
     2521 gcgttagcct cacgtggcgc aaagcgactg acgatatcaa acaccccttt cccgataccg
     2581 gtgatatcta tgccgatata ggtaatgtta aagcgctgca tcagttcgcg gatctgatcg
     2641 gcctgccagg tgaagttaaa tccctgccac tgccagacgg cgagcacacg gaaccgctcg
     2701 ccctcatgga caggcggagc cacaattaca aacgtggaat tatccccgga ccttgacggg
     2761 tcaaagcccg cccacacctc acgattgccg aacggacgtg ccgccgtggg atcgtagtca
     2821 ccccacgtct ccatctccac ctcacacctg accagttcgg agaacttaaa taccgcatcc
     2881 ttgctgtcaa caaactcgca gccgtacagc atcgcatagg tggtcgggtt gtagcggttc
     2941 ctgatacgtt caatatcaac ccgtgcagac aggccacccg ccaccgcctc ttccagccgg
     3001 ataatgtagc gccagatccc gtcagggcat tcacagccca cacgcatcgc gctttctttc
     3061 ggaaactcaa cagcacgacg cgccggatca tccccgcgcc agtcgtcacc attccagacc
     3121 agataagcct ggtgggtttt ggcactgggt gtggaaaaat acgtcgtgcg gtatttgtcg
     3181 tgggtcgcca ttgccgaggc cacttcatgc agtcgcgtga attttgggat ccagaacacc
     3241 tcatcgccgt aaagatggcc gttaaatccc tgggcggtac tggcattggt ggacaggaaa
     3301 cgcagcattg caccattgct cagacggata tgcttacccg tgagcgtgat cccgaactgc
     3361 agggcgaagt taataatata ttcacggaag atttccgcct gtacccgtga cgcagaaaag
     3421 aaaatctggt tatcacctgt caccaccgca tcttcgaacg cctcccatgc aaaataccag
     3481 gtcatgccga tctggcggct cttgaggatg aaacggcgct cacggtgccg gttctcgcgg
     3541 cagtgcagct ggtaggcata aagattttta cgcgcccatt catccagcat ttcagcagtc
     3601 agtccggaaa cgtcgttttt ccggtagcgc cgctttttcc cttctccccc gccgcaggtc
     3661 cccgcagccg cgccgtcctc ataccctgcc ggacgcaggg cggcaatttc cgccatcctt
     3721 tcagcgtgtt tgtttttctg ggcgatgatc ttcacgtgct gtgcgataag ttcacgcagt
     3781 tcggcaagtt cacactctgt cttgccctcc cgtccggtca gcacatcaat acgccgggcg
     3841 atcacgtcct caacggattc agcgggcagc agggtagccc actgccccac atcacaccag
     3901 tggtaaaccg tgcgcggcgg aataccgagt tccgccgcaa tatcgcgggg gtgccaccgc
     3961 ttcaggtaaa gcgatcgcgc cgcctcttt
//
LOCUS       DAZGTJ010000206         3825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000206 DAZGTJ010000000
VERSION     DAZGTJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.207"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..480
                     /locus_tag="ACHK8E_005108"
     CDS             <1..480
                     /locus_tag="ACHK8E_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0347212.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            496..1833
                     /gene="tssK"
                     /locus_tag="ACHK8E_005109"
     CDS             496..1833
                     /gene="tssK"
                     /locus_tag="ACHK8E_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0347213.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1830..2483
                     /gene="tssL"
                     /locus_tag="ACHK8E_005110"
     CDS             1830..2483
                     /gene="tssL"
                     /locus_tag="ACHK8E_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP0347214.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            2486..>3825
                     /locus_tag="ACHK8E_005111"
     CDS             2486..>3825
                     /locus_tag="ACHK8E_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0347215.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLA"
BASE COUNT          756 a          981 c         1154 g          934 t
ORIGIN      
        1 ccggaaactc gggagtttga attcgctaat ctgggcttta ttccgctctc ttactacaag
       61 aaccgggatt atgcctgctt cttctccgct aactccaccc agaaaccggc actgtacgat
      121 acgccggatg caacggcgaa cagccgtatt aatgcccgtc ttccctatat cttcctgctg
      181 tcccgcattg cgcattacct gaagatcatt cagagggaga atatcggtac caccaaagat
      241 cgccgtctgc ttgaactgga actgaataac tggatccggg gtctggtgac ggagatgacc
      301 gatccgggtg atgaactgca ggcctcacat ccgctgcgtg acggaaaagt ggtggtggaa
      361 gacatcgaag acaacccggg atttttccgc gtgaaactct atgctgtacc acatttccag
      421 gtggaaggta tggacgtcag tctgtcactg gtttcgcaga tgccgaaggc aaaagcgtaa
      481 ggcaggcgtc gggaaatgaa gatttatcgt ccactgtggg aagacggggc ttttctgatg
      541 ccccagcagt ttcagcagca ggctgcctgg gatgttcatc tggcagacag cgttgcccga
      601 atggggctgg cgcatccctg gggcgtggtg gctgcagagt ttgatgactc tctgttaccg
      661 ctttcacggt tgaatgccac ccgtctgatt gttcgtttcc cggacggaac gcttattgat
      721 acggaacgtg cggataatct gcctccggtc tgtgatttat cgacggtttc tgagcgttcc
      781 ctggtggata ttgtgctggc gctaccgtta ctgaatgcta acggtggcaa ccttgatgat
      841 ggcagtgaga gcgagcgtcc gcgtcgctgg aaatcagaaa gggtgaatgt gcaggagctg
      901 gccgggcatg agcaaagcga agtggcggta ttgcggcata acctgactct gcgcatggct
      961 catcaggaaa atgctgcctg gctgacctgt cctgttaccc gtcttgttcg agatgcacag
     1021 ggacagtggt gcaaggaccc gcgttttatt ccccctctgc tgacgctgtc tgccagtcct
     1081 tcgctgatga ctgagcttgc ggagttgctt catcacctgc aggcgcgtcg ccagcgactg
     1141 atgtccatgc gtcgggagaa caatgcccgt cttgcggatt ttgcagtggc ggacgtgtcg
     1201 ttgttctggt tgctgaatgc cctgaacagt gcggagcctg ttctgaaaga gttgcttgac
     1261 atgccttacc gccacccgga actgttgtac agggagcttg cccggctggc aggctccctg
     1321 ctcacattct cgcttgagca taatgtggat gcggttccgg cataccgtca tgaaacacct
     1381 gaaaatgtgt ttccaccctt gctttctttg ctgaaccagc tgctggaggc aagcctgcct
     1441 tcccgtgtgg ttttcattga actgaaacaa aatggtgtga tgtgggaggg ggcattacat
     1501 gatgcgcgtt tgcgcgaagg ggcggatttc tggctttcag tgcgctcttc catacccggg
     1561 catgaactgc agacgaagtt tccgcagcta tgcaaagccg gaagccccga tgatgtgtct
     1621 gaggtggtga atgtggcgct gagcggggtc attatccgtc cggttacgca tgtaccggcg
     1681 gccattccgc tccggctgga aaaccagtat ttcgcgctgg atttaagtac ggatgcagcc
     1741 cgggcgatgc tggacgcggg gcgttgtact ttctacaccc cggcatcgct gggggatgtg
     1801 aaactggaac tttttgcggt actgcggaca tgaataaacc tgttatctcc cgggctgaac
     1861 agatttttta tcccggctgg ctgatggtca gccagctgcg ctgcggacaa ccggttgagg
     1921 atggtaaggc cctgtatcgc cgtgcctgtc agctggtgac gcaggcgcgt gaagagctgg
     1981 ctgaagcaga attcagtcag gaaagcagtg aaatcatgtt gtatgccttc tgcgccctgc
     2041 tggacgagag tgtgctgaac cgtgaaaaaa cagacgatgg ctggcacacc tggcagcagg
     2101 acccgctgca ggctcatttt tttggtacgc tcaatgccgg agaagagctc tgggagcgta
     2161 ttcgtgagca actgaaatta cctgcgccgg atgtggcggt actgacatgc ctctgccgta
     2221 cgcttcagct cggttttacc ggtcagtacc ggtcgcagga tgatgagcgt cgcgaagatg
     2281 taattcgcgc actgacagcg cgtgttcctg catttacttt cgcacaggat gcgccggtgg
     2341 tggtgcgtgc accgggttac cgtgctggca gaacgatgta ctggttgtca tgggtggcgg
     2401 gtattgtgac gctggctggt ctctggtgtg tgctttcttc tgtgctcgca gaccaggtgg
     2461 cacggcttac cgggcaggga taaccatgca cgtacgttcc cgtctctggc tgacaatact
     2521 gagtctgttc ctgacactgt ggctgatttc gggattcctg ccactgtctg cagtaaacca
     2581 gataatactg accctggttt cagttgtggc ggcgggactg gtgctgtggt accagtggta
     2641 ttgtctgtct tcccgtcgtg atgccccgga gaaggctggc gatgacggtc tgccgcctga
     2701 atattttcag ggacatgtgc tgcttgttgc cggaaactca gatgcgtggt tcgaacccgg
     2761 tcaggcatac cgggaaacag catcgggatg gtatcttcgt gtggatacgc cggagcagat
     2821 gagaatactg gcagagcggt tgtcgtacag ccgtccggcc ctgtgtggcc aggtcgtggt
     2881 catgctggga cttttaccgg agcagcatac ctcagatgaa gctttcctgt cgtggttgcg
     2941 gaactggcag cgtgcagtca tgcagtgccg gcagtggctg aataccatgc cgccggtgct
     3001 ggtgacattg atggtttcag ggccgtcacc acaaaacaca tccttaaata agcactctgc
     3061 acactggttt acactgacac cggacctgtc gggaatgcac atacgcctga cggaagccgg
     3121 agccattccg gcagaggaat ggctccggga gtgtgatgca gcgtccaggc tccggtgcgt
     3181 cagtgaactg ttatggctga atgccatgtt gtcctggtac aaccgggtta ctgatgccct
     3241 gacagatatt tgccggcagg agcactttta tttccggccg gttgccgttg gattctgtct
     3301 gacgtcggtt gcggcccggc ccggtaatct ctggcaacaa caactgacgt cactcactgt
     3361 actgccaccg cctgccggcg tgtcatcaca ggcactgccg ttgccggata ttctgctgtc
     3421 cggtattccc cgtcatcgtg gactcagtca ctggcacaga ctttgtctcc agacgggggc
     3481 gattgtcggc gtttttcttc tgctggctat gctggcgtct tttatgaata accagcgact
     3541 ggttcgcagc gtgggggaac acctgacggc ttggcaccgg atgcgcggat attcatctga
     3601 atcgaaaaaa actgcactgc aacgtcttca ggctgacagc cagttactga acgactggca
     3661 gcaacgtggt gtgccgcagc gttacggact gggattgtat cagggaatgt ggctgatacc
     3721 gccggttgag cgagcgactg gtgagtggat tgcaccactg cctccccgtc cggtaataca
     3781 gaaagttact cagacgccga aaatggttcg ccttaacagc ctggc
//
LOCUS       DAZGTJ010000207         3798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000207 DAZGTJ010000000
VERSION     DAZGTJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.208"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(5..586)
                     /locus_tag="ACHK8E_005112"
     CDS             complement(5..586)
                     /locus_tag="ACHK8E_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0347216.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            743..3781
                     /locus_tag="ACHK8E_005113"
     CDS             743..3781
                     /locus_tag="ACHK8E_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP0347217.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
BASE COUNT         1307 a          667 c          703 g         1121 t
ORIGIN      
        1 gaatttattt ataaaacgtt gcaattttag catcattttt tattgttctt tttaacctga
       61 agtacgtcga tcgagagata cctgtttttt ctgtcacact gcccagagat tctccctgtg
      121 atataagctc caacgctgta ggcgttcgtg gatgcttctt cctgccaaat tttacgcctg
      181 cggcaagtgc cgctattctt ccgtcgtttg tccgctccag aatacgctca cgttccgctt
      241 cagctacagc agcaaggatt tggataacca ttttgcccat tgtcccttct gtactgagac
      301 cgttctccag gaaccggatg gcgatcccct ttttgtaaca gctatcaacc acacgaatca
      361 tgtcagcagt gttacgcccc agtcggtcca tttttgtaca gatgattacg tcgtctttct
      421 ctgcacgggc caacaaccgc tgtagtcctt cccgttcatc agttgcgcca gtcatcatat
      481 ccgtaaaaat acgatcttcc cgtaccccgg cattttttag ctccgagatt tgatgtgtta
      541 gcttctgatg actggttgat acccgtgcat accccaataa cgccattttt agtctcactt
      601 atcgtttttg acactgcatg gtatcataat gtacaaaata cgatttttga aacctgcttt
      661 tatgctgttt tagacaggta tcagatatct taatatttga tactagccat atttaacaaa
      721 tatttagcat tttggtaaat gtatgcagaa gtacaagaaa agaattagta tattaacaaa
      781 aagtgaaata aatgaattat atcaagtccc ctcttttaat cctgttgaaa gggatgagtt
      841 tttttcgctg gatatcagcc ttaaaagaga aattgataaa ataataaata tagaaagcag
      901 ggtgtatctg atattaataa cagggtattt cagatataaa cctgtgattc ctgaatttac
      961 attaaaagac gtaaaaaggg atgtgaaata tattatccag acatattata aaaacgaaag
     1021 tactaatctc aatttaaaca tacctaaaag tacaaagtac cgccttgtta aacgaatgct
     1081 ttctactcta ggatttgaac aactcacccc atctataaaa aatgagttaa gtgaacgtct
     1141 gaatgatgtt gctactatat gtatggaacc aaaatatatt tttgatgaat tacttgcttt
     1201 tttaggacag cggcgtatag ctcttcctgg atacagtact gtccagaacc tcgtttcaga
     1261 tgctttaaac tttgaaagga aaaggacaat taatattttg tcaaagagaa tttatcctgc
     1321 cactgcacaa aagattacat ccatcatcca tgatgatgga atattaaact ccatcagggg
     1381 atataattat agcacgagag acttctcagc atcagaaata gaaaatgaat taagaactca
     1441 caatgtgata agtagtattt actatgaaat aaaagatata attaattcat taggtatatc
     1501 ccagggaaac atggagtact atgcaactat cgtaaaacac agtacaacat tcagacttaa
     1561 gcgacaccct aaatggcaag gaatacttta tttgtgctgc tacttgtact tcagatacag
     1621 agagataaat gataaaataa tcacagcctt cagatattta gtaaaaaaac ataaagacgc
     1681 ttcagttttg tttgcaaaag ctcgggcaac agaggaaata gatctaataa ataaaaagct
     1741 taagtttgcg agtaaaatat ttaacttctt cgttgatagt aacattgaag acagcatccc
     1801 ttttggatat gttcgtgaaa aggttttttc acttatatcg aaagatgata tgcatcttgt
     1861 gagtgaattt tttgacaaaa ataaaatcga tgtaattgaa tatcaatggc agtatattga
     1921 cagccatcac cttaagatcg ccaattcaat ccgaaagctt tttctggcta tagatatagt
     1981 atatgacaac gagaagtctg ttattggcca gcaaattact gctgccaagg atgatctcag
     2041 aggaaaagga aagctggatg ctaaggatct gagggtaatt catccatccg agaaaaatta
     2101 cattgtaaaa gaaggagaaa ttaacactca acgattcgaa tttcatatgt acagcagggt
     2161 atctaaccat ctggaaaacg gtgtctttta tgctgcggag agtgaacaaa acaagagact
     2221 ggaagatgat ctcatagaac gaagttactg gcataagaat aaatcgattt tgatagagaa
     2281 aacaggcctt gaacgactta tcacaccaat aacgcagact cttaatgagc tggagaatac
     2341 ctttagcgat aaactggctc gtataaccat tggaataaac tcagatgcta atgagtttgt
     2401 gaaaaaacaa ccacactcca acagattaca gtggagtctg gccagtaaaa aatggaagac
     2461 cagcatcgat aatccgattt acaatcagat aaaaaatatc ggaatagttg acgtaatgaa
     2521 gtatgtaaac aatgagacga attttttgtc tgtgtttaat gccatatcat ccaggaaaaa
     2581 taatcttgaa gctgactata atgaccttct tgcctgtata tttggtaatg gcgcaaatta
     2641 tggtgttcac agaatggcat ctgcatcaga ccgtagtatt ggaatactac gtgatgtgaa
     2701 tgacaaattc attcgtccag aaacaacagg aatggccaac acagtaatat ctgatgcaat
     2761 tgcaaatctt tctgtatata aatactggac gataaatgaa gattctccat ttggaagtgt
     2821 tgatgggcag aagcacactt gcagaataaa tacatttaaa gcccgttatt ctgccaagta
     2881 tttcagaaag gggaaagggg tttctgcgat gacactggta tctaatcatg tgccattatc
     2941 aactgtggta atttctccaa atgaatatga aggtcatttt gcatttgact tgttatataa
     3001 caacaccagt gaaatacaac caaagtcatt agcgactgac aatcatggta taaacaacgt
     3061 taattttgcg attcttgaca tttttggcta tcagttttca ccacgttatg caaaattcaa
     3121 gaaaatattt aatgaattat ttgatgttat actggagagt gataaactca ttataaaact
     3181 taaaaaacaa tttaacttta agctcattga acatgaatgg gaaaacatcc aacatattat
     3241 ttgttcttta agcagaaaaa caaccattca aagtacggtt gttaagaagt tatctaatac
     3301 aaagcgtagc aacaagacat tagcagcact tcgggagtat gaccgagtta taaaatgcat
     3361 atatgtcctg gattatgctg acaataaaac cttaaggcaa tttgtgcaac aagcactgaa
     3421 cagaggtgaa gcttatcatc aactaagaag agcaatagca tcagtaaatg gaaatcaatt
     3481 tcgcggtggt aatgactacc aaatagatca atggaacgac tgtgcgaggt taatagctaa
     3541 ttgtgtaatc tattataatt cagcaatatt atccaggctg gtcgagaaat ttgaaaaaga
     3601 aaataataaa aaagcgatag atgttctggc taacttatcc ccagtagcat ggagacacat
     3661 tctccttgga gggaattatt cttttgaaga tcaagttgcg atcacaaatt tggacagtct
     3721 tttggaggga gtagacccac taagtgatat cggagatatt gagtccgaat atgacggtta
     3781 acaacatgat aaataatg
//
LOCUS       DAZGTJ010000208         3743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000208 DAZGTJ010000000
VERSION     DAZGTJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.209"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            96..332
                     /gene="ycgZ"
                     /locus_tag="ACHK8E_005114"
     CDS             96..332
                     /gene="ycgZ"
                     /locus_tag="ACHK8E_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP0347218.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            375..647
                     /locus_tag="ACHK8E_005115"
     CDS             375..647
                     /locus_tag="ACHK8E_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP0347219.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAITDNKSIFNGKKINRIH"
     gene            676..942
                     /gene="ariR"
                     /locus_tag="ACHK8E_005116"
                     /gene_synonym="ymgB"
     CDS             676..942
                     /gene="ariR"
                     /locus_tag="ACHK8E_005116"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP0347220.1"
                     /translation="MLEDTTIHNAIADKALSNYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            1055..1303
                     /gene="ymgC"
                     /locus_tag="ACHK8E_005117"
     CDS             1055..1303
                     /gene="ymgC"
                     /locus_tag="ACHK8E_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP0347221.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
     gene            1639..3162
                     /locus_tag="ACHK8E_005118"
     CDS             1639..3162
                     /locus_tag="ACHK8E_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0347222.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            3294..3512
                     /gene="ymgF"
                     /locus_tag="ACHK8E_005119"
     CDS             3294..3512
                     /gene="ymgF"
                     /locus_tag="ACHK8E_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP0347223.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
BASE COUNT         1089 a          713 c          799 g         1142 t
ORIGIN      
        1 aaaaaattaa tttatcattc tgtacacata tttcgtacaa gtttgctatt gttacctcac
       61 ttaacattga ttaacatttt taacagaggc gtagcatgca tcaaaattca gtgactttag
      121 attctgcggg agcaatcact cgttactttg caaaagctaa cttgcctact cagcaggaaa
      181 ctctcgggga aattgtgact gagattttga aagatggacg tattctgagt cgaaagtcgc
      241 tttgtgccaa acttctatgc cgactggaac aagcgacagg ggaagaggaa cagaaacatt
      301 ataatgcact aattgggttg ctttttgaat aatgcaacag cctgattatt taacaggtta
      361 gctatgaagt cgttatgaag acatctgata atgaaagtac aaaatatgca attactggcc
      421 aggcggtgct ccagatactg cgcatgaaga taaatttttc gttgcagact cttattaagc
      481 aattactcgt aatgaaatct gttgaggaaa atgctttccg acgagattta attgacagca
      541 taattcgtga ttttagcaac agtgatacag gagggccaaa cagaagaaca gcgataactg
      601 acaataaaag tattttcaat ggtaagaaaa taaacagaat acattaaaat ttcataagta
      661 agatgagagg ttactatgct tgaagatact actattcata atgcaatagc tgataaagcg
      721 ttatcaaatt actttcgcag ttcgggtaat ttgttagaag aagagtcagc tgtgttaggg
      781 caggctgtca ccaatttaat gctttcaggt gataatgtta ataataaaaa tattatccta
      841 agtctgatac actccctgga aacaacaagt gatattctca aagctgatgt gattagaaaa
      901 acactggaaa tcgtgttgcg atacacagcg gatgatatgt gacttttaat tattaagtat
      961 aagtttatag aaaactcatt catcttattt ttgtctgtcg ctttagacta tacacagcat
     1021 aattttattg ggttaatatt tctacgaggc tgacatgaat aattcaatcc cagagagatt
     1081 tattttgcaa tgtgctttgt ttaaaaatct cgagagggag gtgttcatga cgcatgggta
     1141 tgttgatagt tatattattg atcaggcttt acgcttgcgt ttaaaagatg aaaccagtgt
     1201 gattctttct gatctctatc tgcaaatatt gcagtacatt gaaatgcata agactacact
     1261 aacggatatc attattaatg acagggaatc cgtgatctct tagaacttga gtaaggttat
     1321 agaataaaca tcagctgtat caccatgctg gtgcaaagtg agcatttcag gcgttatgct
     1381 ttcttaatat atccgcaata tcagggggca aacaggaatg gagagcgctc gtttttcatt
     1441 cttgacgcct gatatcccac ctaatttgtt taattctgcc tacattgtgc tcaataatta
     1501 ataggttaga tgaagttgga gaaaatttta tcttcggcgt ggatatttgt gatgctctct
     1561 accatgctct ctgtagacgc cagcgcgagc gaatgaacgt cattttcatg tattaaaccc
     1621 tttgtccggg acaagcgaat gcgcaataca ctcataccca tcctcgttgc gatatgcttg
     1681 tttataaccg gtgtcgctat tttgaacatc cagctctggt attccgcgaa agcagagtac
     1741 ctggcgggag cgagatatgc cgccaacaat atcaatcata tacttgaaga agcgtcacag
     1801 gcgactcaaa cggcggttaa cattgccggg aaggaatgcg acctcgagga gcaatatcag
     1861 cttggcactg aagcagctct gaagcctcac ctgcgcacaa tcatcattct caaacaggga
     1921 acggtctggt gtacatcctt accggggaat cgggtcctac tgtctcgtat tcctgttttc
     1981 ccggacagta atttactgct ggctccggca atcgacaccg ttaatagatt acctatcctg
     2041 ctctatcaga gccaatttgc agatacgcgc attttggtta cgataagtga tcagcatatt
     2101 cgcgaggcgc ttaatgtacc cttgaaaggg gtaaggtatg tattacgcgt ggcagatgac
     2161 attattggac ctacgggtga tgtgatgacg cttaatggac actatcccta taccgagaag
     2221 gttcactcta caaaatatca tttcactatt atctttaacc cgccgccact ctttagcttc
     2281 tacagactta tcgataaagg ctttgggatt ttgatattta ttctgttaat cgcctgcgcc
     2341 gctgccttcc tgcttgatag gtatttcaat aaaagcgcaa cgcctgaaga gatcctgcga
     2401 cgggctataa ataatgggga gatcgtccct ttttaccaac ctgtggtaaa tggtcgggaa
     2461 gggacattgc ggggagttga ggtgttagcc cgctggaaac aacctcacgg tggatatata
     2521 tcacctgcgg catttattcc acttgctgaa aaatccggat taatcgttcc gcttacgcga
     2581 agcctgatga atcaggttgc cagacagatg aacgctatcg cgagtaaatt gccggaaggt
     2641 tttcatattg gaattaattt tagcgcctcg catattattt cgccgacgtt tgtcgacgag
     2701 tgcttaaatt accgtgacag ttttacccgc cgcgatttaa acctcgttct ggaagttacc
     2761 gagcgtgagc cattaaatgt tgatgaaagt ctggttcagc ggttgaacat tctgcatgaa
     2821 aatggttttg tcatcgcgct ggatgatttc ggtactggct actcagggct ttcttatctg
     2881 catgacctgc atattgatta tatcaaaatt gatcacagtt tcgttggccg cgtcaacgca
     2941 gacccagaat caacccgaat tctggattgt gtattggatc tggcgcgtaa actttcgatc
     3001 agtatcgtcg ctgaaggtgt cgaaacgaaa gaacaacttg actatctgaa tcaaaataat
     3061 atcacatttc agcagggtta ttatttctat aaacctgtta catacatcga cctggtcaaa
     3121 attatccttt ctaaaccgaa ggtgaaggtt gtggttgagt gaaaatcgat cagtaaggcc
     3181 atagtgcggt gtaattatag acagctaatt agctcgttgc ctcttgttac tattgttcat
     3241 tattttgttt gctataattg tttgaaagtt ttgacaggat tgccattagt agcatgaaca
     3301 atagtaataa tctggattat ttcactctct atatcatctt ttccattgca tttatgctga
     3361 tcactctcct ggtcatcctt attgcaaaac ccagtaccgg gctgggagaa gtgcttgtga
     3421 cgataaattt gcttaatgcc cttgtttggc tggcgatcaa tctgattaat cgattaaggg
     3481 aaagactcgt aagccacagg gatcagcact aatctttcag tttctcactg tcagtatgcg
     3541 gctgaatggg ttgctggcag tgaacgcctg gatcattgaa gaaaggaatt attgcgcaaa
     3601 tagttgtcaa ctctggtgtt atcacagttg ttttatatat cattgaaata atttctatgg
     3661 caactattaa tggttttgat gtcgaagagt tatttgttaa acaaaatcgt caccccaaag
     3721 tgatcaatgt catgaaaata agg
//
LOCUS       DAZGTJ010000209         3601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000209 DAZGTJ010000000
VERSION     DAZGTJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.210"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..218
                     /locus_tag="ACHK8E_005120"
     CDS             <1..218
                     /locus_tag="ACHK8E_005120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347224.1"
                     /translation="KNNTSKPPDQQVSHRLSEQQNRFAGGWSHCVTPTTITSVFSRTV
                     VNHKIGTHAGGAGPHDFLFDESENGER"
     gene            509..703
                     /locus_tag="ACHK8E_005121"
     CDS             509..703
                     /locus_tag="ACHK8E_005121"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0347225.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(2259..3275)
                     /locus_tag="ACHK8E_005122"
     CDS             complement(2259..3275)
                     /locus_tag="ACHK8E_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP0347226.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT         1016 a          907 c          771 g          907 t
ORIGIN      
        1 aaaaaaacaa cacatccaaa ccacctgatc agcaggtttc tcaccgactt tctgaacaac
       61 aaaaccgctt tgcaggtggt tggtcccact gcgtgacacc aaccaccatc accagtgttt
      121 tttctcgtac cgtcgtcaat cacaaaatcg gaacccacgc cggaggcgcg ggaccccacg
      181 attttctgtt tgacgagtca gaaaacggcg aacgctgaca gatttcaatc tggctgcgtg
      241 agacgttggc ggggtgtcgg ggcgcagccc tgaccaggcg gcatttgtct gattgcgtgt
      301 gcgcggtccg acaaatacac atcctgtccc gtttttcgca cggtttttat tgattaccgg
      361 acagatgaac ccggattgcc atgttgcgaa aatcacgtta aaacgattac gagcgtgcag
      421 ttgggctgat atatcaacag tcatgccaat atgtattaac ccgacaggcc gctctttttt
      481 aattcaaata aaaatataaa ttacgagtat gaatcaggac aaagcaattt atttagaaaa
      541 taaatatttt tcacccgcaa acgaagctgt cagactggag cgtgaaggcg attacattgg
      601 agcatggaaa aaatggaatg atgcgcttca ttatgccaaa ggagaaaata tagactggtg
      661 tatagccaga gctgaatatt gtaaaaagac aggtcggttt taattctgac ggggtttcaa
      721 aggggcgtcc cctttggcac gtcgacgtat caaaatgcgc cagcattttg tatacagtcg
      781 gtcacgtgcc tccaggattt accatcaaaa acgcccctgt agttaatcct gaatcaagat
      841 ataactaaga tataattagc gtataactaa ggcataacta aagtataact aacttagtgt
      901 gcatataatt aagatataac taatatataa ctaatgtata actaacggtt caccaacgga
      961 tcgcatgtag tagacgctac ataaaaaatc ctgaaatgga ggattcgtca taaagagatt
     1021 cctgttttta gcgttctgat gcaggatagg gcaaacaccg caaaatgacg tctttggtat
     1081 gttttgtgat gtttgcttct ggcatctgtt atctgtttta ttgggtgttt ttatgagtgt
     1141 tgatgtaatt aaacaaattc agagtataca ggacaaagga attattattt attcaaaata
     1201 cagagctaac gaatttaaca gggatgatgt atacagagag agttacggcc tgattattga
     1261 gtttaatcat cttattgctg aacatgccgt tcatgatgaa aaactgatta accatactgt
     1321 ctgtattctg aatgaattac gaagcatagc gataaattaa aaaatccccc tggattacag
     1381 ggggaagcca tcagagtttg agtaatgaga aatattattt tggagagagc accacaaatg
     1441 cgctcttttc caatctgccg taaccatcgg taaaagacaa tcaggacagg cgaaaaaatc
     1501 cccctatatg gcaagggggt gtcaatgagg acatggcaat tgtgagtttt gtgtgtttta
     1561 tgtgttgcag tttgcaacgt cagaaatttg ccgtagttat acgtgaaaaa caatcctgat
     1621 ttctggacag gatgtgtaat cgtataaccg cgcacgcacg ccgatacgat taccacctgg
     1681 tcaggtcttt gcccgacacc ctcgacaccg ttcccgcgat acgatagaac atcagggcgc
     1741 gtttccgggt aaacgcatgt taatgaccgg gaattcattg attagggacc ggagacgcgc
     1801 tacaaaactc ctgcgcgttt ctggcatcaa ctgaccatgg ggatggtttg gcagtagcag
     1861 caagcgccag aaacgcgctc aaaatcatca gatgtacagc gaacaaaaaa ttgtcatcgg
     1921 caaaaaatca cgttaaaaca atcgcgagca ccttcctgtc tccttcaatt acggtcaaac
     1981 attttgcatc tgtcgggaag ttgctcacct ccataattca atttctcatt gacaataatt
     2041 ccggtgatat cccctctttt gaggggcttt ttttacccac acaccacaaa cagcagccat
     2101 accatcgtca aaaacgcgct caaaacgtct ctgacgtcct gaaccaacca aaacatacaa
     2161 aaccattgcc caatgcaaaa aagtgcgtta gaaagcgtct gagcgcgttt ctgacgtctt
     2221 ttttgttgct gtgtcgctac tcgttgtcag attcgcgctc aatccttcat cctgtcagct
     2281 tcaggattcc gcttgtaacg tttttccgta cgtccccagt caaacgccag ccagaaggct
     2341 tgctcctcga actcgtccag ttctgccgga atatctgccc cgatcaaatc ctcgtccgtc
     2401 tccggtttat cgactttcag aacctctttg agacgaccgc cggttgccac catgcgaagc
     2461 ccataggtct gccgcgccag ctccagaaac cacgccggat cacgcacaac ttccgaagat
     2521 ttcaccgtgt atttcatcgt ctcaatgacg cctgactcca gcgccgcacg atgcatatca
     2581 ggcagggtta caccatccgg ccgtttccag ccctgtttcg gttttaccac ccgaatatcc
     2641 acacccgcct cataatcgac acgaaggcat tcactccaga tctccaccca gcgagcatgt
     2701 ttcacataac cattaccgga caacatcgaa ggggggacca tcatcagaac gtgaaaatgc
     2761 ggatggcaac atcctggcac cttcccgtaa gtaatttccg tcgtacgtaa ccagccaaga
     2821 actgacctga attccttacg atcactcaag cgtttccatg ctgcattcat ccgaatgagt
     2881 gtttcgcggg tattttccac agggggattt ttgacagtca gcgtcatgaa caaccagcgg
     2941 gccgcaggac gttcaaccag caactttggc aaggcctccc agaggcgacg catccagcgg
     3001 agagagcgac gacgactaca caccggacaa aacggaacat gacaaaactc ccccttccga
     3061 agggccagct tcatttcgcc cgtcaggcgg gacaccttcg gcgcaaacaa caaccgacgg
     3121 gaacaatcaa acatccgttc tccacgagag gaaaacatcg aatcgctttc gtaaatctgc
     3181 gccaccctgt cagccatcac tcgatcactg tcccattttg tatcccgcgt gctgtaatcg
     3241 cgtaacgaga gtaccggact gtctggattt ttcatttgca ccccccaaga actgggcaga
     3301 tgcggaaatt ttgtgttagc atttttctgt cacctgtgac tctgttgccg aacaacgaat
     3361 tccaaactgg tgatcgcccc ctggagtgtg accgcactcg caggggaact ccctcaaaac
     3421 tgttcatttt ttaaccagaa gtttttatct cttttccacc cttttttacc actcaaagcc
     3481 gcgccaccac tggactaagc caaaataaca cagaccgcag ttgttgccta tagtataaat
     3541 acaaggatcg cgcgcgcgat cgcccaccca aaatgcgcgt tttttgagcg ttcccgagaa
     3601 c
//
LOCUS       DAZGTJ010000210         3461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000210 DAZGTJ010000000
VERSION     DAZGTJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.212"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..475
                     /locus_tag="ACHK8E_005123"
     CDS             <1..475
                     /locus_tag="ACHK8E_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0347227.1"
                     /translation="CLLNVFVSTVKQLKNSIIAISAIVAMATVMDVCGLIGTLAQSLV
                     DLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHVDPLWLAAANTSGA
                     TGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLKVGLVYHLFMA"
     gene            525..1244
                     /locus_tag="ACHK8E_005124"
     CDS             525..1244
                     /locus_tag="ACHK8E_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0347228.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            1255..2670
                     /locus_tag="ACHK8E_005125"
     CDS             1255..2670
                     /locus_tag="ACHK8E_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0347229.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            2675..3370
                     /locus_tag="ACHK8E_005126"
     CDS             2675..3370
                     /locus_tag="ACHK8E_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0347230.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
BASE COUNT          934 a          722 c          885 g          920 t
ORIGIN      
        1 atgcctgtta aatgtatttg tcagtacggt aaaacagctc aaaaactcca ttattgccat
       61 ttctgcgatt gttgctatgg caacggtaat ggatgtttgt gggttgattg gcactctggc
      121 acagagcctt gtagatctca ctggtggggc ctacctcttc attgctccgg ttatcggcgc
      181 tcttggtaca tttgttactg gcagtgacac aaactccaac attctgtttg gtaaattgca
      241 gaccactgcg gctgaaaaat tgcatgtgga cccactctgg ctggctgccg ctaacacatc
      301 tggcgcaaca ggcggcaaaa tgatttctcc tcagagtatt gctatcgctg tttcagccac
      361 ccgtatggat ggtcagggga gcgtaatcat gtcagggaca ctgaagtact gctttgctta
      421 tattgttatt cttggactga aagtaggcct tgtctaccac ctgttcatgg cctgagaaaa
      481 aataacaaac ggacgtggaa tattaatcaa caggagaaat atctgtgaat gttaactttt
      541 tcgtcacctg cattggtgat gcatttaaat cccgtatggc acaaaacagc gtgctgttac
      601 tggagcaatt gggttgcaat gtccatttcc ccgaaaaaca gggatgttgc ggtcagccag
      661 cgataaatgg cggttatgtt cgtgatgcca ttcctggtat gaaaaatctc atatcttcgc
      721 tggaagagaa tgactggccg gtagtatctc ctgccgggtc atgcacttac acgataaaaa
      781 attacgctcg ctatctcagt gaagaacctg agtgggcggc acgagctgaa aaagtagctg
      841 aacgcctgca tgatttgacg tcatttatag taaatcatct tggcatcaca gatgttgggg
      901 caagactcaa tggtcgcgca gtgtaccatc cttcatgcag cctttcccgt aaactgggtg
      961 taacccgaga accgcttttg ttgcttgaga atgtacgggg acttgagctg ctgccgtttc
     1021 acgctcagga aacgtgctgc gggtttggcg gcacgttctc tgtaaaaatg gcggagatct
     1081 ctggtgaaat ggtgaaagag aaagtcgggc atattatgga tgttaaacct gattttctta
     1141 tcggggctga cgccagttgc ctgctcaaca tcggcggtcg gcttagccgt gaagggcatc
     1201 ctgtaaaagt gatgcatatt gctgaagtcc tgatgcacag atgaggtaaa cagaatgtct
     1261 ttgaaaacaa gtgatatcag gtttaaagag cgtatccgta ttcagattca ggacccgatc
     1321 atgcgaaaag ctgtggctaa cgcacaggag cgcataggga ctaatcgaca gaaaatggtt
     1381 gatgaactgg ggcactggga agaatggcgt tcacttgctg aacagattcg tcggcatgtt
     1441 ctggaaaatc ttgatgctta tttgtatcaa ttgtccgaaa aagttactgc gaatggcgga
     1501 catgtatatt ttgcccgtac cagagaggag gcaaccagtt acatactgaa ggtggcgaaa
     1561 gaaaaaaacg ccaggaaggt tgttaaatcc aaatcaatgg tgactgagga aattggtatg
     1621 aacggcgttc ttcagaaagc cgggatacca gtgatagaaa cagatcttgg tgaatatatt
     1681 cttcagctgg cgcatgagcc gccatcgcat gttgttgtgc cggcaataca taaagatcgc
     1741 catcaaattc gcagagtttt gcatgaacat ctggggtatg acggccctga aactccagaa
     1801 gcaatgacat tgttcatccg tgagaaaatc agacaggatt ttctgagtgc tgaagttggc
     1861 gtaacagggt gtaactttgc cgttgcagaa acaggctcag tttgccttgt gacaaacgaa
     1921 ggtaacgcac gtatgtgcac cacgttacct aaaacacata ttgctgtgat gggtatggag
     1981 cgtattgcac caacattccg ggaggtggat gttcttatca ccatgcttgc aaggagtgct
     2041 gtgggagcaa gactgacagg ttataacacc tggcttacag gtccaagaga agaaggacat
     2101 gttgacggcc cggaagagtt ccatctcgtc attgttgaca atgggcgttc agaggttctc
     2161 ggctcagaat tcagcgatgt attgcgctgt attcgctgcg gtgcctgtat taatacctgt
     2221 cccgcttatc gccagattgg tggacatggc tatggctcaa tctacccggg cccaatcgga
     2281 gcagttattt ccccgttgct tggtggatat gaagacttta aggaattacc ttatgcctgc
     2341 tcattgtgca ctgcctgcaa tggggtatgt ccggtaaaaa tacctctggc acaactaata
     2401 caaaaacatc ggcaggttat ggcagagtcc gaaataacac catcaacaga acgccgtata
     2461 accagaatat tcaattacgt aaacagtcat ccggcattgt ggaaagttgg catgattgct
     2521 ggcgcgaaaa cagccagcct gtgcatcagg aacggaaaaa cgccgattag cccaggtgct
     2581 attaaagaat ggtctgtggc cagagactta ccttctcctg atggagaaag cttccgtagt
     2641 tggttcaaaa gacataaaaa aggagggtaa ctttatggaa aacagagagg aatttctcgt
     2701 tgctatcgcc agaaagcttg gcaggccagt aagacgtatt cccgaagcaa tgccggagcc
     2761 tgtaagtacg ctggctacga cacgtttaac cgagttgaca atggaccaac gctgtgaggc
     2821 ttttattaaa tttgcctcag aagtgatgct tgcgaagtgc gttctggttt cgcctgaaga
     2881 cgcaccatca aaagcattag acatctgttg gacgttcggt tccgggccag tcattatcag
     2941 caacgatcaa cgactgtttg atacggggat tgcaccattg ctacaaaaag agatgggcgc
     3001 tgtcctgtgg accccggaga agggtgagga aaatattcat cttgcagaaa aagcaaaaac
     3061 aggcgtcgtt ttcgctgagt atggactgac agaatctggt ggtgttgttt tattctccgc
     3121 ccctgaacga gggcgttcag taagtctttt acctgaagcg tctgtgtttg ttttacgcaa
     3181 aagtaccatt cttcccagag tggctcagct tgcgcaaaaa cttcatgata tggctcagga
     3241 aggacaacgt atgccttcct gtatcaatct gattggaggc ccaagttcta ctgcagatat
     3301 agaactgatc aaagttgttg gtgtgcatgg acctgtacat gcaacttact tgattattga
     3361 tgactgctaa tacattttag tatgtatata tgattaacag ctccgtttag ctgagctgtt
     3421 aatcattcat ttagttgtat tagtcgtgac ttgagcgtaa g
//
LOCUS       DAZGTJ010000211         3291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000211 DAZGTJ010000000
VERSION     DAZGTJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.213"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            187..1869
                     /gene="ydeN"
                     /locus_tag="ACHK8E_005127"
     CDS             187..1869
                     /gene="ydeN"
                     /locus_tag="ACHK8E_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0347231.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1921..3078
                     /gene="ydeM"
                     /locus_tag="ACHK8E_005128"
     CDS             1921..3078
                     /gene="ydeM"
                     /locus_tag="ACHK8E_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0347232.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3044..3166)
                     /locus_tag="ACHK8E_005129"
     CDS             complement(3044..3166)
                     /locus_tag="ACHK8E_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347233.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          999 a          705 c          658 g          929 t
ORIGIN      
        1 gattagttgt ttatctgcga gaaattactt agtagaatag aaagtaatgt caacagttta
       61 tggactgtta tttctctttt tttagcttaa tctgctatct ctttatgtga ctaacttcac
      121 ttacatccac ttatttctct tcgtaaaatt actttggaat taagtacaat aagaagagga
      181 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
      241 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
      301 aaaacaaacg ttgctttctc agactttacg ccgactgaat acagtaccaa gggaaagcca
      361 aatattatcg tactgactat ggatgatctt ggttatggac aacttccttt tgataaggga
      421 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
      481 gataaagcca ttgaagctgc gcaaaaatca acgccgacgc tcctgtcatt aatggatgaa
      541 ggcgtacgtt ttactaacgg ctatgtggcg cacggtgttt ccggcccctc ccgcgccgca
      601 ataatgaccg gtcgagcgcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
      661 ggtattccgc taacagaaac tttcctgcct gaattattcc agaatcatgg ttattacact
      721 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
      781 caaacgcgtg actatcatga caacttcacc acctattctg cggaagaatg gcaaccgcaa
      841 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
      901 ccttcactgt tcaaaaaccg tgaacgcgtc cccgcaaaag gttatatcag cgatcagtta
      961 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     1021 tacctggctt ataatgctcc gcatctgcca aatgataatc ctgcaccgga gcaatatcag
     1081 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     1141 gatcagggtg taaaacgcat tctcgaaaaa ctgaagaaaa acggacagta tgacaatact
     1201 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     1261 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     1321 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     1381 tacccgacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggc
     1441 gtttccttgc tgccctggtt gcaagataag aaacaaggcg agccacataa aaatctgacc
     1501 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     1561 cacaaatttg tccgccatca gtcagacgat tacccgcata accccaacac cgaggactta
     1621 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaat
     1681 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     1741 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga caacagccag
     1801 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     1861 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     1921 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     1981 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     2041 gagttcatca aacaatatat cgcagcgtct ggcaatcagg tctattttac ctggcaaggc
     2101 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     2161 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     2221 gaatggtgtg cctttctcaa agaacatgag tttctggttg gtatctcgat cgatggcccc
     2281 caagagctac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     2341 atagcagcca tcgagcgtct gaaatcatat caaatagagt ttaatacgtt aaccgttatt
     2401 aataacatta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     2461 aaacatatgc aatttatcga attgctcgaa accgggacgc cgaatattga tttcagtggt
     2521 catactgaga atacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     2581 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcatccgt
     2641 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc ataccagttg tattttccag
     2701 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     2761 tttgtctatc cacagtacaa aattggaaac attaataaat cggaactcaa aacgatgaac
     2821 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgtatat
     2881 aaacctatct gcaatggtgg ttgccctaag catcgtatta ctaaagtaaa caatgagact
     2941 atttcctatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     3001 gccatggtgg agttagccaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     3061 caaatggaga ataattaata acgctccttc tttgctccag tcttagcctc cgtcttatga
     3121 ccttaacaaa gcacccgaca taaattcctg ttgggtgttt cgtcatcatc atcttccctt
     3181 gcgaaaattc tggcttacgc ggcatgcgtc agggcattag caataccctt gattatcaat
     3241 agcataacca taaccactga aaatgaaata tttctttata tttcatattg t
//
LOCUS       DAZGTJ010000212         3269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000212 DAZGTJ010000000
VERSION     DAZGTJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.214"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            214..1734
                     /locus_tag="ACHK8E_005130"
     CDS             214..1734
                     /locus_tag="ACHK8E_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP0347234.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1760..2098)
                     /gene="maoP"
                     /locus_tag="ACHK8E_005131"
     CDS             complement(1760..2098)
                     /gene="maoP"
                     /locus_tag="ACHK8E_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP0347235.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2217..3056
                     /gene="hdfR"
                     /locus_tag="ACHK8E_005132"
     CDS             2217..3056
                     /gene="hdfR"
                     /locus_tag="ACHK8E_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP0347236.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3151..3226)
                     /locus_tag="ACHK8E_005133"
     tRNA            complement(3151..3226)
                     /locus_tag="ACHK8E_005133"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3191..3193),aa:Trp,seq:cca)
BASE COUNT          880 a          801 c          803 g          785 t
ORIGIN      
        1 atagtgagtg aaaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc
       61 ctttgcccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
      121 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
      181 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
      241 gcagccctgg gagtaaatgc gcccccaatc actgttgagg tacatatcag taaaggtcta
      301 ccaggcttaa cgatggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg
      361 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
      421 gctccagccg atctgccaaa agaaggggga cgatatgatt tgcctatcgc tattgcgtta
      481 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
      541 ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtgc aactgaagcc
      601 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aggatgaagt ggggctaatt
      661 aacggtgaag gatgcctgat agccgatcat ctgcaggctg tctgtgcgtt tctggagggt
      721 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
      781 ctcagtgatg ttgtcggtca ggagcaagga aagcgaggac tggaaattac cgccgctggc
      841 gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
      901 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
      961 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccatt ccgctcacct
     1021 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
     1081 atttcgctgg cgcataacgg cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt
     1141 acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga
     1201 gcaaaaataa cctatccagc ccgtttccaa cttgttgcgg cgatgaatcc cagccctacc
     1261 ggacattatc agggaaacca taaccgctgc acaccagaac agacattacg ttatctcaac
     1321 cggctctcgg ggccctttct cgaccgcttc gatctctcac tagagatccc attaccgccc
     1381 cccggcattt tgagtaaaac ggtagtgcag ggagaaaaca gcaccaccgt taaacaacgt
     1441 gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaacgc ctggctggat
     1501 aatccggaaa tacgccaatt ctgcaagctt gagagcgaag atgcgcagtg gctggaagaa
     1561 acgctgatcc atctggggtt atcgattcgt gcctggcagc ggttattgaa agttgcacga
     1621 accattgctg atattgatca gtctgacatt atcacacgtc agcatttgca ggaggcagtt
     1681 agctatcgtg cgattgaccg tttgctcatc catctgcaaa aactactgac ataaaaaaaa
     1741 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
     1801 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttgatacgc gtcatatact
     1861 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
     1921 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
     1981 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa gtcaccatga cgagagaatc
     2041 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
     2101 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
     2161 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
     2221 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcag
     2281 ctgaatcgct ctatctgacc caatcagccg tgagctttcg aatcagacaa ctggaaaatc
     2341 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgctggtg
     2401 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgtaaggagg
     2461 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
     2521 gtatgcttaa tcagtggctg ggacgcttgt atcaaaatca ggatgtccat acaggcttac
     2581 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
     2641 ttgatcttct tattactact gaagcgccca aaatggacga atttagcagt cagttgctcg
     2701 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
     2761 atctgcgact ggaatggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
     2821 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgacgc
     2881 ttaatggttg cacctggcta cccgtcagtt gggcgcgtaa aaagggcggc ctgcataccg
     2941 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
     3001 aaaatgcgtt gattcgcgat ctattgaaaa ttaacgtgct ggatgaagtg tattaatctg
     3061 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggttattt cagataaaaa
     3121 aaatccttag ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa
     3181 cacccggttt tggagaccgg tgctctacca attgaactac gcccctaatt agggtggcgg
     3241 aacggacggg actcgaaccc gcgaccccc
//
LOCUS       DAZGTJ010000213         3256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000213 DAZGTJ010000000
VERSION     DAZGTJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.215"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1329
                     /locus_tag="ACHK8E_005134"
     CDS             <1..1329
                     /locus_tag="ACHK8E_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP0347237.1"
                     /translation="SFEKGTNYADLQRIINPQFSFNLSVVLRCPRAVTDYLIAVSPAS
                     YQITSPRNNEPDTLKELVVTDPLSTLLNEILQLKESSVLPDEITVLVPTDLVKNSLQN
                     DMRTLGVNIETVSRFRGMESSVVITLYSESMEDAQLFCAYSRATTAFIAIYDSEKLAW
                     REKNKFLHKLVNRKEVQRAINEAKHNSSSSVLMAGFFREGELKLSTVKLAWSKHFSSW
                     LVEFDERNSPEEMWIDYLYSYLPYPIIYWFRDSCRAVNYIKNKQFELNELGSGIHLVI
                     KNCSICKKMVPFYSDDNCLLCNKKNEELSPTRDEINKIREFDVTISQDNLNSVTQRLL
                     PTPLAAVRARRVAFENPYRNKVLSESLPAGNLLYRSALAFIQALIALVPSKKILDLDK
                     ITDALRRRYVHLEQLEYAELRNALACALSTCYNRKYLIKVAKGKYLPVDD"
     gene            1411..1758
                     /locus_tag="ACHK8E_005135"
     CDS             1411..1758
                     /locus_tag="ACHK8E_005135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0347238.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            1931..2095
                     /locus_tag="ACHK8E_005136"
     CDS             1931..2095
                     /locus_tag="ACHK8E_005136"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0347239.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(2099..2566)
                     /locus_tag="ACHK8E_005137"
                     /pseudo
     CDS             complement(2099..2566)
                     /locus_tag="ACHK8E_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2630..2914
                     /locus_tag="ACHK8E_005138"
     CDS             2630..2914
                     /locus_tag="ACHK8E_005138"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0347240.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(3131..>3256)
                     /locus_tag="ACHK8E_005139"
     CDS             complement(3131..>3256)
                     /locus_tag="ACHK8E_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0347241.1"
                     /translation="LLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          985 a          664 c          711 g          896 t
ORIGIN      
        1 tcctttgaaa aaggaacaaa ttacgctgat cttcagcgaa taataaatcc ccaattttct
       61 tttaacttat ctgttgtgtt aagatgtcct agagccgtta ctgattattt aatagctgtc
      121 agccctgcct cataccaaat tacttcccct aggaataacg aaccggatac gctaaaagaa
      181 ttagttgtta cagatccact ctccacattg cttaatgaaa ttttacaatt gaaagaaagt
      241 tctgtactgc ctgatgagat tactgtcctt gttcctacgg atttagtgaa aaattccctc
      301 cagaatgata tgagaacttt gggggtgaat attgaaacag tatctcgttt tcgaggtatg
      361 gaatcatctg tcgtgattac attgtattcc gaaagcatgg aggatgcaca gttattttgt
      421 gcttattcaa gagctacaac tgcttttatt gcaatttatg attcggagaa attagcatgg
      481 agagaaaaaa ataaattttt gcataaactg gttaacagaa aggaggttca gagagcaatt
      541 aatgaagcaa agcataattc ttcctccagt gttcttatgg ccggtttttt ccgcgaagga
      601 gagttaaaac ttagtactgt aaaattagca tggtcgaagc acttttcttc gtggcttgtt
      661 gaatttgatg aaagaaattc tccagaagaa atgtggatag attatttgta ttcctatttg
      721 ccttacccga ttatatattg gttcagagat tcatgtagag ctgttaatta cataaaaaat
      781 aaacaatttg aattaaatga attaggctca gggatacatc tggtaataaa aaattgtagc
      841 atatgtaaaa agatggtacc tttttattct gatgataatt gtttgctttg taataagaag
      901 aatgaagagt tgtcaccaac cagagatgaa attaataaaa ttcgcgagtt tgatgtaaca
      961 atatcccagg acaatcttaa ttcagttact caacgattgt tacccacacc attagctgcg
     1021 gtaagagccc gaagagttgc atttgaaaat ccatatagaa ataaagtact aagtgaatca
     1081 ttacctgctg gaaaccttct ttacagaagt gcattagctt ttatacaggc cttgattgca
     1141 ttagtcccat caaagaaaat actggacttg gataagataa cagatgctct gcgtaggcgg
     1201 tatgttcatc ttgagcaatt agaatatgcc gaattaagaa atgctttagc ttgtgcattg
     1261 tcaacttgtt ataatagaaa atatctcatt aaagttgcaa aaggaaaata tttacctgtt
     1321 gacgactagg tgcgtattac cgtgaaggag atcggtgagt aaccccctgt gtcagcatag
     1381 ttgtcgcctc aattttttaa ggagataaca atgccaggac aaccaatacc aagtgagctc
     1441 aaagagaaag gactgatatg gcttttacca ccatactgct ggtcacatcg tcagatagcc
     1501 cgtaagctaa agatcagtgc ctcagttgta tcaaaatgga gatatgagtt agtggagcaa
     1561 gggcttttac ctgaaaatga aaagattacc tccgttgatg ttgaagactg gacaccggag
     1621 cgtcgattct cagttgttct tgagacggct acgctgtcag aaatggagct tgctgattat
     1681 taccgacgca tgggattgtt tgttgaacag gttaaagaat ggcgggctat ctcaattcag
     1741 gcgcatgaaa aaaattgacg gaaaatattc gaacagacag ggcattgcgt gacgcaaaaa
     1801 ataagttacg agaactgtag aaagagctta agcgtaaaga taaagctctg gcggaagctg
     1861 cggccctgct tatattgagg gaaaagttca atgccctctg ggacaacagc gaggaagact
     1921 gacccctctc ctggagcggc gtaaaattat tgcccttgtc cgcgaggccg tctccaacgg
     1981 cgcccggaaa agtcaggctt gtaggtgtat cggtatttca attcggacac ttcagcgctg
     2041 gactgatggc aacgttatta ccacggatgt aagcgtcaac ggagcaccgt attgacgctt
     2101 atttattggt gagtactacg ttccatggca ggagttcatc aactcggttg gaaggccatt
     2161 ccggcagtac gctcaggata aggcgcagat acgcttccgg atcgataccg ttcagacggc
     2221 aggtgccgat cagcccgtac aacagtgctc cacgctcgcc gccgtgatcg ctgccaaaga
     2281 acataaagtt tttctttccg agacagactg cacgaagcgc tctttccgca gcattattat
     2341 ccgcctccgc cagaccgtca tcactgtaat aacagagggc atcccactga ttcagtacat
     2401 agctgaacgc ttcgcccagt ctggattttt tcgacagcgt accattcttc tccaccatcc
     2461 attcatgcag cgacgtcagt aacactttgc ttcgctgctg cctgacggaa agacgctctg
     2521 actccggtaa tccccgtatt tcgtaagcgt aaaccgaccg ccgtatgtag ccattaagcc
     2581 agtattggta acgtaggtgc ccaccttttc aaactagtgg acgcctgtta tggagcaaaa
     2641 aacattatcc gcagaacccc gcagatcatt ttcaaataat tttaaacttc aaatggttaa
     2701 actggcttta caaccaaggg tttctgttgc ccgtattgcc cggaagcatg atatcaatga
     2761 taatcttctg ttcaaatggc tcaggctctg gctgatcaaa gggcgtatat cacggcgtct
     2821 tccggtaagg aactcttctg gcattggcgt tgaattatta cctgtggaga tgacgctgga
     2881 tgagcggcct taccccgctt tttacgcgag gtgattatga agatcaatga ctagccgtcg
     2941 aaccgtgtgc atgaactgct gcactgaaac ttctcagctg taaaataatc cttacgctac
     3001 gtacttcccg gagcgcttac gattaatcgg atgcagtcct gactggaaga aagaaaaatg
     3061 ggaaaaaata agaggaatta atatcctcca cgttcctgaa atcctggagg atattaatgt
     3121 catcaaaata ttagcgaacg ataatcccta acaaaatacc cagtgcgcca aagtcagcat
     3181 cactgaaggt ggtgttagca aaaccaatgt tacccagcac aggcaataag aatactggca
     3241 ggaaggtaat aagcaa
//
LOCUS       DAZGTJ010000214         3256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000214 DAZGTJ010000000
VERSION     DAZGTJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.216"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            664..1440
                     /locus_tag="ACHK8E_005140"
     CDS             664..1440
                     /locus_tag="ACHK8E_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0347242.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            1437..2108
                     /locus_tag="ACHK8E_005141"
     CDS             1437..2108
                     /locus_tag="ACHK8E_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0347243.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLNSLKEYCDVVIYIKSFNEVKYYSVVTDALAEYIID
                     TKIQV"
     gene            2134..3069
                     /locus_tag="ACHK8E_005142"
     CDS             2134..3069
                     /locus_tag="ACHK8E_005142"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0347244.1"
                     /translation="MSIIVNHVMSSDIVSGIFTDLLSYYKRYCDRDISIVESSNAIDN
                     ADVYHYHRPHLESRLKENSVVTIHHDLYDTDPWLDYDKFHPRYMEAKKILCLNSSQQN
                     YLNNKGLMNTEIIPHGYNSDIFCSVGAQKKIKNKVCIGFISKRYGRKVKGEAYLYEIF
                     KRLDSKRYRFIFVGVDRTITAYKASRFGFEVKCYERLPYYCYGDLYKQLNFLLVTSLY
                     EGGPANIPEAIASGTPIISTPVGMVNDYVRDGINGLILTGDIDNDIDSINNFSQKSKY
                     QKLSSNALEMSGNIMSWQDVMQKTSLVYKELAGCA"
     gene            3070..>3256
                     /locus_tag="ACHK8E_005143"
     CDS             3070..>3256
                     /locus_tag="ACHK8E_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0347245.1"
                     /translation="MKNISILFADSIHFHVRNFKSLFDLIDRHKIHHTFIHERNKWVS
                     AYGNYEEFFNELQPLYNK"
BASE COUNT         1045 a          523 c          663 g         1025 t
ORIGIN      
        1 tgtaaattac tttggtcagg tccttcacag cagaaacaga atgtcatgaa agataaattt
       61 acgcagtcac ttgatcaact gcttagtttt gcgtcttaaa ggattgtaac tatggcaact
      121 gtggagcgaa aaatcattgg gcacgggaaa agtgcggtca tatagaaaag tgctctctat
      181 tcagcatctc atggtttatc agcgaagaca gaaaaaaatt ataacgatgc gcaggaattt
      241 gtggaggttt gctaatatag agcgattcga gcatccctcg ttaaatctct cgaaccgcag
      301 tatattcaga catttttaaa atacgccaga tgattacgac agaaagaaca caacatataa
      361 ttcatgttcg ctgtcctctt gctgaatacg gaattgtctt caataaaggt gctacacatt
      421 atggtgagag ctaccacggt ttcttgagga tgcagaaaat ggatttaccg atatcgtgca
      481 attttgtatc ggtagtatgt ccgactcatg tctcatggat aatgaaccat atattataac
      541 tgcgggtaga aaaatgcgca tctaacgact ttgcctggac gtgtcgatta actgttcctg
      601 ggtttgctac tcttgtcagt cagacgataa aagtgagcat aaatatatag gattacatat
      661 caagtgaaaa gaagtggatt tgaagttcag aaggtgacag tagaggcatt atttctacga
      721 gaaatacgaa cacgttttgg taagtttcgt ctgggatatc tgtgggcaat cctggaaccc
      781 gctgctcatt tgctggtgct gttaggtatt tttggctata ttatgcatcg tacgatgcca
      841 ggtatctcgt tcccggtatt tttacttaat ggtttgattc ccttttttat ctttaatagt
      901 atcagtaatc ggtctgtgga agctattgat gcaaaccagg ggctttttaa ttatcggcca
      961 gtaaagccca tggatacaat aatagccaga accctgcttg agacactaat ttacattata
     1021 gtttatgtgt tgcttatgct tattgtctgg atgtcaggag aatattttga aataataagt
     1081 ttattacaac tggtacttgt atggactctt ctgattatta tttcatgtag tgtcggatta
     1141 atatttatgg tcgttggtaa aactttccct gaaatgcaaa agattctacc gatatttctt
     1201 aaaccgctgt atttcatttc ctgtatcatg ttccctctac attcgatccc acagcaatac
     1261 tggacatatc tactctggaa cccattagtc catgtcgtag agttaagtcg tgaggcagtt
     1321 atgccgggct atatcagcga aggcgtgagt ttgaactatc tggcaatgtt cactctggtc
     1381 accctgttca tcggtctggc gctataccga acgcgtgaag aggcaatgct gacatcatga
     1441 ttaagattga gaatctgacg aagtcatatc gcacgtcaac aggtcgacac tatgtgttta
     1501 aggatttaaa tatcgaactc ccttcaggaa aaagtgttgc ctttattggt cgtaatggtg
     1561 caggtaaatc aactttactg agaatgattg gcggtattga ccgccccgat agaggaaaga
     1621 tcatcaccaa taaaacgata tcatggccca ttgggctcgc cggtggattc cagggcagtt
     1681 taaccgggcg agaaaacgta aaatttgttg cacggttata tgcgaagcca gaagaactga
     1741 aagataaaat tgagtttgtc gaagaatttg ccgaactcgg taagtacttt gatatgccga
     1801 tcaaaactta ctcatctggt atgcgatctc gcctgggttt tggtttaagc atggcattta
     1861 agtttgatta ttatattgtc gatgaagtaa cggcagtcgg tgatgccagg tttaaagaaa
     1921 aatgtgcaca ttactttaaa gaaaggcata aagagtctag ttttttaatg gtttctcata
     1981 gtttaaattc tttgaaagag tattgcgatg tggttatata tattaaaagt tttaatgaag
     2041 taaaatacta cagtgtagta actgatgcgc tggcagaata tattatcgac actaaaatac
     2101 aagtataaac tttccaagta acaaggcgtt attatgtcta taattgtaaa tcatgttatg
     2161 tcttctgata tagtaagtgg aatatttaca gatcttctct cttattacaa aagatattgt
     2221 gatagagata tatcaattgt tgaaagtagc aacgctattg ataatgctga tgtctatcac
     2281 tatcatcggc ctcatttaga aagcagactt aaggaaaact cagtagtaac aatacatcat
     2341 gacttgtatg atacagaccc atggttggat tatgataagt ttcatccccg ttatatggaa
     2401 gcaaaaaaaa tactgtgcct taactcctct cagcaaaatt atttaaacaa taaaggctta
     2461 atgaatactg aaattatacc acatggttac aattctgaca tcttttgctc agtaggggcg
     2521 caaaaaaaaa taaaaaataa agtttgcatt ggttttatat caaagagata tggacgaaaa
     2581 gtgaaaggag aagcttatct ttatgagatt tttaaaagat tagattctaa aagatacaga
     2641 ttcatctttg taggagtcga tagaacaata acagcatata aagcatcaag atttggtttt
     2701 gaagtaaaat gttatgaaag gcttccctat tattgttacg gtgatttata taaacaactt
     2761 aattttcttt tagtaacgag tttgtacgaa ggtggaccag caaatatacc tgaagcaatt
     2821 gcatccggta cgccaataat atcaactcct gtgggtatgg taaatgatta tgtcagggat
     2881 ggtattaatg gtcttatatt aactggagat attgataatg atatcgactc tataaacaat
     2941 ttctctcaaa aaagtaaata tcaaaaatta tcaagtaatg ctcttgaaat gtctgggaac
     3001 ataatgtctt ggcaggacgt tatgcaaaaa acatcattgg tatacaaaga gttagcaggg
     3061 tgcgcttgaa tgaaaaatat aagtatatta tttgcggaca gtatacattt tcatgtgcgt
     3121 aattttaaaa gtctattcga tttaatcgac aggcataaaa ttcaccatac gttcatacat
     3181 gaacgaaata aatgggtgag tgcttatggt aattatgagg aattctttaa tgaattacag
     3241 ccattatata ataaat
//
LOCUS       DAZGTJ010000215         3226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000215 DAZGTJ010000000
VERSION     DAZGTJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.217"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            193..711
                     /locus_tag="ACHK8E_005144"
     CDS             193..711
                     /locus_tag="ACHK8E_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0347246.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            921..3029
                     /locus_tag="ACHK8E_005145"
     CDS             921..3029
                     /locus_tag="ACHK8E_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0347247.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          817 a          810 c          922 g          677 t
ORIGIN      
        1 aacttattgc tgaataagag atttaatgat tttaaaaata gtttttacga caaccgtaaa
       61 ttctagctcg gtttgtatgt aaaaaatact gaaaaatgat gcggttccgt gaagagagct
      121 gcatctgcct gtctgggatt aacggcaggt cattgtaagt agtaattaac cacgttaaca
      181 aggagtttac aaatgccaac accgtgttat atctccatca ctggtcagac ccagggaaat
      241 attactgcag gtgcatttac cgctgattct gtcggcaaca tctatgtgca ggggcacgaa
      301 gacgagatgc tggtgcagga attcctgcat aacgttactg tcccgaccga tcctcagtcc
      361 ggccagcctg cgggtcagcg tgctcacaag ccgttcattt ttaccgtggc gctgaacaaa
      421 gctgttccgc tgatgtacaa cgcactggcc tccggcgaaa tgctgccaac cactgaactt
      481 cactggtggc gtacctctgt ggaaggcaaa caggagcatt atttcaccac ccgcctgacc
      541 gattcgacga ttgtcgatat gaaactgcat atgccgcatt gccaggatcc agcgaaacgc
      601 gagttcaccc agttgctcga agtttcactg gcctaccgca aaatagagtg ggaacacgtc
      661 aagtctggca cctccggtgc cgacgactgg cgcgcaccgc tggaagcata agttaagcca
      721 acagcatccg gctatcaggc cggatgctgt ttttgtatta cgccgtaaaa agaaaggttg
      781 tcagtaaggg attacgttca cgggagatgg cggatgccgg aaacttcggc ggtagcgtgc
      841 ctgaacgcaa gcgtaacact gtactgttac agccgcttta acagcgaact gtctgttttt
      901 ccttaaaccc ggaggttgct atgtcaaccg gattacgttt cacgctggaa gtggacggtc
      961 tgccaccgga cgcgtttgcg gtggtctcct ttcatctgaa ccagtcactc tcttcgctct
     1021 tttccctcga tctctctctg gtcagccagc agtttctctc ccttgaattt gcgcaggtgc
     1081 tggacaaaat ggcctacctg acggtatggc agggcgatga cgtacagcgc cgggtgaaag
     1141 gtgtggtgac ctggtttgaa ctgggggaga acgacaaaaa ccagatgctg tacagcatga
     1201 aggtgtgccc accgctgtgg cgcacagggc tgcgccagaa cttccgtatc ttccagaatg
     1261 aggacatcga aagcatactc gcgacgatcc tgaaagaaaa cggggtgacc gagtggagtc
     1321 cgctgttcag cgagccgcat ccttcccgtg agttttgtgt ccagtacggc gaaaccgatt
     1381 acgatttcct gtgccggatg gcggcggagg aaggcatctt cttttatgag gagcacgcgc
     1441 aaaaaagtac cgaccagagc ctggtcctgt gcgacaccgt gcgttatctg ccggagtcct
     1501 ttgagatccc ctggaatccg aacacccgta ccgaggtgag caccctatgc atcagccagt
     1561 tccgctacag cgcacaaatc cgcccatctt ccgtggtgac caaagactac acctttaaac
     1621 gacccggctg ggcagggcgt tttgatcagg aaggccagca ccaggattac cagcgcacac
     1681 agtatgaagt gtatgactac cccggacgtt tcaagggtgc ccacgggcag aactttgccc
     1741 gctggcagat ggatggctgg cgaaacaacg cagaagtggc gcgcggaaca agccgttcgc
     1801 cggagatatg gccgggacgg cgaattgcgc tgacggggca tccgcaggcg aacctgaacc
     1861 gggaatggca ggtggtggca agtgaactgc acggcgagca gccgcaggcg gtgccgggac
     1921 gcaggggttc aggtaccacg ctgaataacc actttgcggt aataccggca gacagaacat
     1981 ggcggccaca gccgttgctg aaaccgctgg tggacggccc gcagagcgcc gtcgtgacgg
     2041 gaccggcagg agaagaaatc ttctgtgatg aacatggccg cgtgcgggtg aaatttaact
     2101 gggaccgcta taacccgtca aaccaggaca gttcatgctg gatccgtgtg gcacaggcgt
     2161 gggcaggcac cggctttggc aacctggcga taccgcgtgt gggtcaggaa gtcattgtgg
     2221 acttcctcaa cggcgatccg gaccagccga ttattatggg gcgtacctac caccaggaaa
     2281 accgcacccc cggcagcctg ccgggaacaa agacgcagat gaccattcgt tcgaaaacct
     2341 ataagggcag cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct
     2401 acatccacgc gcaaaagaac atgaacaccg aggtgctgaa taaccggacc acggatgtga
     2461 taaacaacca tgctgagacc atcggcaaca atcaggtgat tgcggttacc aacaatcaga
     2521 tacagacggt gggcgttaac cagatagaga cggtgggcag taaccagatc attaaagtgg
     2581 gctctgttca ggtggaaacg attggacttg ttcgtgcgct gaccgtgggc gtggcgtatc
     2641 agacgacggt aggtggcatt atgaacacct cggtggccct gatgcagtcc tcgcagatcg
     2701 gtttgcataa atcgttgatg gtcgggctgg gttatgacgt caaagtcgga aataacgtta
     2761 ccttcaccgt tggtaaaacg aaaaaggatg ataccggaca gaccgcgatt tactccgccg
     2821 gtgagcatct ggaactctgc tgtggtaagg caaggctggt gctgactaag gacggacaaa
     2881 tttttctcaa cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac
     2941 tgttgattaa ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc
     3001 aggatacgcc ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac
     3061 aagcattaaa aataaacaga agaaggaatt gaatcatgag cggaaaacca gcggcgcgtc
     3121 agggagatat gactcagtat ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg
     3181 gcgcgcccac cggcgtggcg tgctcggtgt gtccgggcgg gatgac
//
LOCUS       DAZGTJ010000216         3192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000216 DAZGTJ010000000
VERSION     DAZGTJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.218"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..82
                     /locus_tag="ACHK8E_005146"
     CDS             <1..82
                     /locus_tag="ACHK8E_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFP0347248.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(397..1383)
                     /locus_tag="ACHK8E_005147"
     CDS             complement(397..1383)
                     /locus_tag="ACHK8E_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0347249.1"
                     /translation="MYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1484..2218
                     /gene="nanR"
                     /locus_tag="ACHK8E_005148"
     CDS             1484..2218
                     /gene="nanR"
                     /locus_tag="ACHK8E_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0347250.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(2254..>3192)
                     /locus_tag="ACHK8E_005149"
     CDS             complement(2254..>3192)
                     /locus_tag="ACHK8E_005149"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0347251.1"
                     /translation="LMELIMNNDKSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIY
                     QDPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYRAVMLDKEGTALAP
                     EFLSQGYTLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGF
                     SAGGHLASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPDSRKQLL
                     GDNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMH
                     LYEKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT         1008 a          712 c          566 g          906 t
ORIGIN      
        1 attaacgtcc aatgcccttg ttgggttatc gacctgcgcc acatcgactg aatgttttgg
       61 ccctgacaga aaaaagaact aataggtgat gtttccgtcc agagatactc tggatggagt
      121 gtcggcgtca gtgatataag actcgagtga acactgagcc gacctcgaaa tgacgttgta
      181 ggtgcagaca tgccataatg ggactgtcga tgatattaca gggactcacg tccctggtgc
      241 gtgccttcgt gacacactac cgtccacata gtatcagaac cagtgatatc aaacggtaac
      301 gaccagctat cttggtgttg ataccgacaa ctccatacca ctccgtcatg tctctcagag
      361 aatcatgtca gagctgtaga tatatcctga acttgtctac aaataatttc ttattttttg
      421 cgcataacat tcagcaataa ctccccttct ggccattgaa ttaaagtgtg tacttctctg
      481 tgacgttgtc cagcttaatt ctgtcctcca tgatgacccc agatacccga cagataaatc
      541 atcggcatct tcttctggct cattagttaa ttcatgtcgg ttactatcct gaaagctgag
      601 aaaatgtatt tcatcatcag tccgttgtct gtaatgtcca tatataaaat catattcttt
      661 ttgataagat tccttccagt accaggttgt atcaccacac aaccatggaa ctctttctga
      721 cgaattacca acacactgag atgagtattc agataactca gacctgaaag aagttaccat
      781 ctgataaaac aagtcaggat gcttttcata atctggtgac ataagatcaa actccccctg
      841 catccagcag acagatagca gtttattctt tctgttatat tccagagcta ccttaacccg
      901 ggctactaag tcctcataga gtgctgttcc ggtgccccac cgcattgcat cagctgttgc
      961 ccctgtatca ggtacataca taccttcagc tccaacagta aaggcggcac ctcctctgca
     1021 acaaggaacc agtaatatcc ctgcttgctc aggtatgtat ggcagtaact ttttagctat
     1081 atgcaatccc tgtccgacac aaccatattc ccctttatgc aaatcagcag ccggatggtg
     1141 atatccactc atatcctgaa catcatgcag acagtgatct gccggaataa tctcattgaa
     1201 tttacactct tttcctcctg gtgtgattgt ttttcgacga gccagttgtt ttacacggga
     1261 ctcgggtttg tcgagtgtat ctggcagggg aataccttca ccgtaggcca taccattaga
     1321 ctgtcctgcg agggctatca cataccagaa atctattttt tcattaaaat cataacgata
     1381 catcactccc ccctttacat aatagattta attaaatatt ttcattatta aaagaaagtg
     1441 ttaaattaaa aactcatttg ctctaatcca tataaatcaa tatatgtccg acaacaaaaa
     1501 aaccgtgtta cctgggctaa caacgaccag aaagcccatt gttacaaaaa aactttcaga
     1561 aatcgttgcc gaagagcttg aaagaatgat ccgccaggag gagataaggg aaggtgaata
     1621 cctgccttct gaacgtgaac ttatggcatt ctttggcgtt ggaagaccat ctgtcagaga
     1681 ggctttatca tctctcagac ataaagggct aatccggtat aacaagggag aaaaagccag
     1741 tgtctgccgt ccatcaccag aaaccatcat taatgggtta tcaggcattg ttaaagattt
     1801 tttatccaca gctgatggca tcaaggattt tgaaagacta cgcatctttt ttgaaaccaa
     1861 ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat attcacaggc ttgagaaggt
     1921 gctcagtatc catcataact cactgagaac ccccgaagtg tatgctcaat atgatattga
     1981 atttcatagt atacttgctg atatacctga caataaagta ttaaaaacaa tacactcagc
     2041 attttctgaa tggctgataa tggccaaacc tctggaaaag aataaaatga catatgaaaa
     2101 taacaaaaaa acccatcagc agcatattga aatacttacg gccataaaag agcatgattg
     2161 tgataaagct gaacagctaa tgtataatca cctttataaa cgaattgaag catcatagtt
     2221 actcgacgct tcaccaggta gtcaccataa atattattta aatccatgag aatacatcca
     2281 gtccataaac agttttatcc atcctccaac aggaagatct gtggcccact gaacactaaa
     2341 gccatgccct cctttctcat agagatgcat ttcagcagaa acgcctttct ctattaggcc
     2401 acggtacatg agcagactgt tttcaattga cacaagatca tcatcacaac aatggagtaa
     2461 aaacacagga ggaaaacatg atgtgacatt aagatccgca ctatattgta ttatattttc
     2521 tgaggtacta ttgtccccca gtaattgctt cctggagtct ggatgtgtca catcatcttt
     2581 cattgatatt acaggataaa ttaacccgac aaaatcggga atagcgaaaa tctcttgttc
     2641 tttactgtca gaagaacgtt tgaaatcaca aatctgattc acaaacgttg aggcgagatg
     2701 ccccccagca gaaaatccta atacaccaat acgatgacgc tgtagatgcc atttatcagc
     2761 attcttacga atataaagca ttgcagatat tgcatcaata aaagttttgt ttatattttc
     2821 ttcctcagct ggcgatggta atctgtaact aagaacaaat aatgtatagc cctgagataa
     2881 aaactctgga gcaagagctg taccttcctt atctaacata acagcccggt agcccccccc
     2941 gggaataatc aatattcctg ttccatttgg tttatctggt aaaaaaataa ttaactgagg
     3001 agcataaata tctgtcaata cacgatcaag ttcaaattcc ccgttatgct caggatcctg
     3061 ataaatacgg cctgaaagag atttactctc agatattgtc tcacttgaca agtcaacaat
     3121 agttgattta ttaaacaagc ttaacatcat ttcatttaaa gatttatcat tattcattat
     3181 aagctccatt aa
//
LOCUS       DAZGTJ010000217         3167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000217 DAZGTJ010000000
VERSION     DAZGTJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.219"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1925
                     /locus_tag="ACHK8E_005150"
     CDS             <1..1925
                     /locus_tag="ACHK8E_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFP0347252.1"
                     /translation="HSAIHDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNM
                     MRKAVKEHFKLEKEYLTQRPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEA
                     NELLKTEKDEFYRNYLEKTVKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLS
                     LENPRRFIFSKWTLREGWDNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVK
                     NRDFTLNYYVDFTEKDFVDSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTL
                     MNEIFDAEIIDDNENFKDSDAYSRLKAKYPAAFPAGVKPSKIKKATDGKKRTKMRIGK
                     FSELKELWDLINQKVVIEYKINSENEFLSIFKSFMIEETDRFTKSGVHTRIDRIYIHN
                     DTAMSKSIVSDDDNFAKLNTMSYREFLDNLSQTIFAKHDTLHKVFCDIKDTINITDYL
                     NIQTIRKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKFTNADGNHLHEVLSGDLGV
                     LQDNTKAPLDSYLFEEVFYDSELERLNITDGEIHSVAVFTKIPKNSIKIPVAGGYTYS
                     PDFAYVVKTTKGDYLNFIIETKNVEGKESLRKEELDKIKHAQKLFEQVSKSVKVTFIT
                     QFSGDRIHDLIKNVIQ"
     gene            2009..2707
                     /locus_tag="ACHK8E_005151"
     CDS             2009..2707
                     /locus_tag="ACHK8E_005151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347253.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     AVSLTDKGFTESSACNIVNLDLMLLPEENWLLYKENYNTGYQDALYKSVWRMHTYEYP
                     TATISCLTKLNNENILNIITGIEVILLAAKKPACKDRTDKIAEMLKIPFSVVKEFIDE
                     QALLNKSNIQGTGS"
     gene            complement(2754..2906)
                     /locus_tag="ACHK8E_005152"
                     /pseudo
     CDS             complement(2754..2906)
                     /locus_tag="ACHK8E_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
BASE COUNT         1110 a          565 c          586 g          906 t
ORIGIN      
        1 cgcatagtgc gatccatgat ttaactcttg atgctttaaa taagaataca gcagtgttga
       61 gtaatggtat tgagctaaaa attggtagct cgataaaccc ctattcttac gatcagacac
      121 ttgctgataa catgatgcgt aaagctgtca aagaacactt taagttagaa aaagagtatc
      181 tgacacaaag gccacgcata aaacctctta ctcttttctt cattgatgac attgaaggct
      241 atcgtgatgg taatgatatt gcaggaagcc tgaaaactaa gttcgaagaa tacgttttgg
      301 cagaagcaaa tgaactgtta aaaacagaga aagatgagtt ttacagaaac taccttgaaa
      361 aaactgttaa ggatatatct tctgttcatg gtggttattt ttcaaaagat aatagtgata
      421 aagatgataa aattgagcag gaaatcaacg aaatccttca tgataaagag cttctattgt
      481 ctttagaaaa tcctcgccgt ttcattttct ctaaatggac gttgcgtgag ggatgggata
      541 acccaaatgt ctttcagatt tgtaagctcc gttcgagcgg tagcaccaca tctaaacttc
      601 aggaggtagg gcgtggttta cgtcttccag tgaatgaata catgtgccgt gtgaaaaacc
      661 gcgattttac tctcaactat tatgttgatt ttacggaaaa agattttgtt gattctcttg
      721 taaaagaggt taatgatagt tctttcaaag aaactgttcc aggtaagttt actcaagaac
      781 ttaaggataa aatcttatct cagtatcctg agctttcatc cagaacactc atgaatgaga
      841 tctttgatgc tgaaattatt gatgataatg aaaacttcaa agattccgat gcctatagcc
      901 gtttaaaagc aaaatatccg gctgcattcc ctgccggggt gaaaccaagc aaaataaaaa
      961 aagccacgga tggaaaaaag cgtactaaaa tgcgtattgg taagttcagt gagttgaaag
     1021 aactatggga tttgatcaat caaaaagtag taattgagta taaaataaat agtgaaaatg
     1081 aattcttatc cattttcaaa tcattcatga ttgaagaaac agatagattt actaaatcag
     1141 gtgttcatac tcgcattgat agaatttata ttcataatga tacggcgatg tcgaaaagta
     1201 tcgtcagtga cgatgataac tttgctaaac taaacacaat gagctatcgg gaatttcttg
     1261 ataacttatc acaaactatt tttgctaaac acgatacctt acataaggtt ttttgtgata
     1321 taaaagacac tatcaatatc accgactacc tgaacattca gacaatcaga aagattaaat
     1381 ctgggtttag caagtatttg ttgaacaatt catttaacaa attcagcctt ggatataatg
     1441 tgatttcagg ctcaattcat cctacaaaat tcactaatgc agacggtaat cacctgcatg
     1501 aggtattatc aggtgatctt ggagtattac aggataatac aaaagccccc ctggactctt
     1561 atctttttga agaagttttt tatgattcag agcttgaaag actcaatata acagacggag
     1621 agatccactc tgttgctgtt tttacaaaga taccgaaaaa ctcaattaaa attccggtgg
     1681 caggtggata cacatattct cctgactttg cttatgttgt aaaaacaaca aagggtgact
     1741 accttaactt tattattgaa acaaaaaatg tcgaaggtaa agaaagttta cgtaaagaag
     1801 aactggataa aataaagcat gcacaaaaac ttttcgaaca ggtaagtaaa tctgtaaaag
     1861 taactttcat aactcaattc tcaggtgaca gaatccatga tctgataaaa aatgtgattc
     1921 agtaaacaca tgcatgataa caaagtggag gtggtttcac ccccacttta taattataca
     1981 cacatcacaa aattcgcagg tgagttcaat gacaacatgt tctgaactga aggaaagagc
     2041 ccgttttgat ggtatgcatg atgcttctct tgaagtggca tggcaattac ttatgacgaa
     2101 aagtgtatcc actatcacta ctgagataat atccggtgca gacatgtcca atttttcagt
     2161 gattatcagg gaacgatata aacatgcaat agcaaaatct cttctcaatc atttatctcc
     2221 cgatgttgtt tctgacatca tcggtttatc tcatgatgag ttcaatcaac aatataaagg
     2281 taatttaacc agacaacaac acctgagaga tattgctgta agtttaacag ataaaggatt
     2341 cacagaatca tctgcatgta atattgtcaa cctggaccta atgttacttc ctgaagaaaa
     2401 ctggttactt tataaagaaa attacaatac aggatatcag gatgcacttt ataaatccgt
     2461 atggagaatg cacacatacg aatatccaac agcaaccatc agttgtttga caaaattaaa
     2521 taatgaaaat atattaaata ttattacagg tatagaagta atactcctcg cagcaaaaaa
     2581 acctgcatgt aaagacagaa cagataaaat tgctgaaatg ctgaaaatac cattctctgt
     2641 cgtcaaagaa tttattgatg aacaggcttt attgaacaaa tcaaacattc agggaactgg
     2701 cagttgaaca aaacgccagt ccgaagactg gcgcagtgat aaaacattct gcatcatacc
     2761 acattctgct cctgaagatg atgcagacga tgctcccagt ttcccgggta atacccgggc
     2821 gtcagcagta cgcggcgtcg ctcattacag agcgagttac caatcactac ttctccggcc
     2881 acgccacaca gcgacagttg tatatagggg tttggggagc aatgaaacag taaacgccat
     2941 taagaggtta ataaaaatag ccaattattt attaatcaat aaattatatc tattcattga
     3001 acgctctcag ctccccctta ctggggtccg cctggaattc agaaaataaa ccacgttaag
     3061 actgtttttt agccaaatga ctttcaggga tcgcctgaat ggcgggacaa cagcggtttt
     3121 ggtgtccgaa atcgtacaat tctgtacatc tacacaaggg aattatc
//
LOCUS       DAZGTJ010000218         3090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000218 DAZGTJ010000000
VERSION     DAZGTJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.220"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(229..305)
                     /locus_tag="ACHK8E_005153"
     tRNA            complement(229..305)
                     /locus_tag="ACHK8E_005153"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Asp,seq:gtc)
     gene            complement(438..1169)
                     /gene="dnaQ"
                     /locus_tag="ACHK8E_005154"
     CDS             complement(438..1169)
                     /gene="dnaQ"
                     /locus_tag="ACHK8E_005154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP0347254.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1234..1701
                     /gene="rnhA"
                     /locus_tag="ACHK8E_005155"
     CDS             1234..1701
                     /gene="rnhA"
                     /locus_tag="ACHK8E_005155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP0347255.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1698..2420)
                     /locus_tag="ACHK8E_005156"
     CDS             complement(1698..2420)
                     /locus_tag="ACHK8E_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0347256.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2454..>3090
                     /gene="gloB"
                     /locus_tag="ACHK8E_005157"
     CDS             2454..>3090
                     /gene="gloB"
                     /locus_tag="ACHK8E_005157"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP0347257.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQ"
BASE COUNT          848 a          738 c          718 g          786 t
ORIGIN      
        1 catgaaatat acattcattt aatcaatata aaattatcct ctaaaaatat atttaagttg
       61 aatggttcga tatcattatt gaattttaat aaatgagaca acaagcgaga gttacgcact
      121 tacgccagag taaaatcatt gaacagagaa atggcgggat tcaaatacaa aaaaaccgca
      181 aaaataaaaa tttttgcggt tctctgaact cattttcatg agtgaatttg gcggaacgga
      241 cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc
      301 gctccgcgtt ttgttccgtt gggaacgagg cgaatattac ggattgcctc acccttcgtc
      361 aacggttttt ctcatctttt gaatcgtttg ctgcaaaaat cgcccaagtc gctattttta
      421 gcgcctttta caggtattta tgcccgccag aggcagcttc cccccttctt ctgcaccaga
      481 tcgagacggg cttcatgtgc tgcaagctct tcatctgtcg caaaaacaac gcgtaactta
      541 cttgcctgac gcacaatgcg ctgaattgtt gtttcatctt gttgctgttg tgtctctcct
      601 tccatcgcaa aagccatcga cgtttgccca ccagtcatcg ccagataaac ttccgcaagg
      661 atctgggcat cgagtaatgc cccgtgcagc gttcgtttac tgttatctat ttcgtagcgg
      721 gcacataacg catcgaggct gttgcgctta ccgggaaaca ttttcctcgc caccgcaagg
      781 ctatcggtga ccttacagaa ggtattggtc ttcggaatat cgcgcttaag caacgaaaac
      841 tcgtagtcca taaagccgat atcgaacgct gcgttatgga tcaccagctc cgcgccgcgg
      901 atatagtcca tgaactcatc ggctacctct gcaaacgtgg gcttatcgag caaaaattca
      961 tcggcaatac catgtacgcc aaaggcttcc ggatccacca gccgatcggg tttgagataa
     1021 acatggaagt tattgcccgt caggcgacgg ttcaccactt caacggcacc aatctcaatg
     1081 atcttgtggc cttcatagtg cgcaccaatc tggttcatac cggtagtttc ggtatcgaga
     1141 acgatctggc gtgtaattgc agtgctcata gcggtcattt atgtcagact tgtcgtttta
     1201 cagttcgatt caattacagg aagtctacca gagatgctta aacaggtaga aattttcacc
     1261 gatggttcgt gtctgggtaa tcctggacct gggggttacg gcgctatttt acgctatcgc
     1321 ggacgcgaga aaacctttag cgcaggctac acccgcacca ccaacaaccg tatggagttg
     1381 atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt gcgaagtcat tttgagtacc
     1441 gacagccagt atgtccgcca gggtatcacc cagtggatcc ataactggaa aaaacgtggc
     1501 tggaaaacag cagacaaaaa accagtaaaa aatgtcgatc tctggcaacg tcttgatgct
     1561 gcgctggggc agcatcaaat caaatgggaa tgggttaaag gccatgccgg acacccggaa
     1621 aacgaacgtt gcgatgaact ggctcgtgcc gcagcgatga atcccacact ggaagatacg
     1681 ggctatcaag tcgaagttta agcctgtggt ttacgacatt gccgggtggc tccaaccgcc
     1741 tggcgaattc gtggcttgtt tttactctgt ttcatcggat ttagcgttaa aggaatggtc
     1801 cgtttccggg caacaataag ttgtaagcaa ccaagcgcag gaatatgcgc attcaatagt
     1861 tttcctccgt gtttgttcca cgggagaacg tggaaacggc tggcgtgtag cacttcaaaa
     1921 ttcaacaaag agagccagtc cagctgccgc atcagagtaa acatccggct gttatagggc
     1981 gaggttttgc gcaataccgg cacaagtttg cgtaatccca ttaaactgat gggattgaag
     2041 ccactaatca ccagccagcc atcatcaatc aatacccgat cggcttcacg caataaacga
     2101 tgcggatcgg tgcaccacgg caatgtatgt gccagtagac aaacatcaac ggatttatcg
     2161 gcaaaaggaa gatgaagtgg gtccgcctgc acctggacgg gcagtccttg cgcagaaaca
     2221 ttcacttgat gagaaaccgc gcacgcttcg caattgattt ctgcgcttaa attgccaatc
     2281 ttaagcagat gaaaaccata cattttagtg aaccacgggt tgagctggcg ctccagcgcc
     2341 ttgcgataaa gctctcccca gggcaaatcg ccccagcaat caggagccac gacagtttga
     2401 gggactcttg ccggtttcat cacaaccttc cgtttcacac tgagaggtaa tctatgaatc
     2461 ttaacagtat tcccgccttt gatgacaatt acatctgggt tttgaatgat gaagcaggtc
     2521 gctgcctgat tgtcgatccc ggagacgcag agccagtatt aaacgccatt gccgccaata
     2581 actggcaacc ggaggccata tttctcaccc accatcacca cgatcacgtt ggcggcgtaa
     2641 aagaactggt gaaaaagttt ccgcaaattg tggtgtatgg tccacaagag acacaagata
     2701 agggaacaac acaggtagtc aaagatggcg aaactgcctt cgttttgggg catgaattta
     2761 gtgtaattgc cacgccgggt cacactttag gacatatctg ttacttcagt aaaccttatc
     2821 tattttgcgg cgacacgctg ttttctggtg ggtgtggtcg gttgtttgaa gggacagcat
     2881 cacaaatgta tcaatcactt aaaaagttaa gtgcgttacc tgacgataca ttggtatgtt
     2941 gtgctcatga atatacctta tcaaatatga agtttgcttt gagtattctt ccgcacgatt
     3001 tgtccataaa tgattattat cgtaaagtta aggagttacg ggcaaaaaat caaataacac
     3061 tacccgtaat tctgaaaaat gagcggcaaa
//
LOCUS       DAZGTJ010000219         3024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000219 DAZGTJ010000000
VERSION     DAZGTJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.221"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            63..2099
                     /locus_tag="ACHK8E_005158"
     CDS             63..2099
                     /locus_tag="ACHK8E_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0347258.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFISIDENELNQLKTI
                     CDEIFGEANFIENIVWNKRIPKNDKGIGNIHEYILAYAKNNEISKEFLMRKDGIDDVY
                     QFIEKLKKEKVPLDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADS
                     FGPTYEVLHPLTNNPVKIPDRGWRWKEGTFNHIAKRIDGKYADIKKLHDGSVICGGIW
                     FARTEKTQPSSVKFLDEVEEFLLRSIISTKSDGGVEVENLFGGKGYFSYPKPTSLIKT
                     LFGSVKTEDKDIYLDFFAGSGTTAHGILELNIEDGRKRNFICVQLDEETDKKKQAYKE
                     GYKSIFNITKDRIIKAGKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTLSNLSFF
                     DDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPNFTSEAL
                     KALLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            2102..>3024
                     /locus_tag="ACHK8E_005159"
     CDS             2102..>3024
                     /locus_tag="ACHK8E_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0347259.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDGKEAT"
BASE COUNT         1046 a          515 c          581 g          882 t
ORIGIN      
        1 atatgctcgc ctgaactctc tccgtcaagt acctctacct ccgtgctttc tggaggtgct
       61 gtatgaatat acgcaacaat catctaccgg gaacagaaac tcttttttct gaaactgaaa
      121 ctgcgaacag taaacaactt gctgttttga aaactaactt tccacagtgt ttcgataaaa
      181 acggagcttt cattcaggaa aagctgcttg agatcatcag agcatcaaat gttgaaatct
      241 ctaaagagtc atactgctta aactggcttg gcaaatccta tgctcgcctg ctggctaacc
      301 taccaccaac gacattgctg tcagaagata aagaccataa cagacgtgaa gagaacaaga
      361 acagtcaaaa tctgttaatc aaaggggata accttgaggt attaaagcat atggttaatg
      421 cttattctga aaaggttaaa atgatttata tcgatccgcc atataacacg ggttctgatg
      481 ggttcgccta taatgatgat cgcaaattta caccagaaca gctttctgat ctggcaggta
      541 tcgatcttga tgaggctaaa cgtatacttg aatttaccac gaatggttca agcagccata
      601 gcgcatggtt aacgttcatt tatcctcgtt tatatattgc tcgtgagctt ctacgtgaag
      661 atggtgtgat atttatttcg attgacgaaa atgaattaaa tcaacttaaa actatttgtg
      721 atgaaatttt tggcgaagca aatttcatag aaaatattgt atggaataaa cgtatcccta
      781 aaaatgataa gggtattggt aatatccatg agtacatcct tgcttatgcg aagaataacg
      841 aaataagtaa agaattttta atgcggaaag atggtattga tgatgtatat cagtttattg
      901 aaaagttaaa aaaggaaaag gtgccattag ataaagcaga gcagcaactc aaaaaattct
      961 acagctccaa cggatatgat cgtggaatta ctctttacaa cggtttgaac gaggattata
     1021 ggccatgggg taaaattaat atgagttggc ctaatgcaga ttcatttgga cccacgtatg
     1081 aagtattgca ccctttgact aataaccctg tcaaaatccc agacagaggc tggcgttgga
     1141 aagaaggcac ttttaatcat attgcaaaaa gaatagatgg aaagtatgca gatatcaaaa
     1201 aactacatga tggtagcgtt atttgtggtg gaatttggtt tgcccgtaca gaaaaaaccc
     1261 aacccagttc ggttaaattt ttagatgaag ttgaagagtt tttactaaga tcaattattt
     1321 caactaaaag tgatggtggt gttgaggttg aaaatctttt tggtgggaaa ggatatttct
     1381 cctacccgaa gccaacaagt ttaataaaaa cactatttgg ttcagtaaaa accgaagata
     1441 aagacattta tcttgacttt tttgctggct ctggaactac agctcatggt attctggagt
     1501 taaatattga agacggcagg aaaagaaact tcatctgtgt tcaattagat gaagaaacag
     1561 ataaaaagaa acaagcctat aaagaagggt ataaatccat cttcaatata acaaaagaca
     1621 gaattattaa ggcaggaaaa aagctgaaaa aagataatcc taattataat ggagatttgg
     1681 gatttaaaat atttgaaaca gttcatgatt ttcgagcgaa agatgagtct gaacttacgc
     1741 tttctaatct tagtttcttt gacgatgctg tattgacacc agagcagtat gacactctat
     1801 tgacgacatg gtgcgtgtat gatggcagct tgctaacaac accaattgaa gatattgacc
     1861 ttaatggcta caaagcacac ttgtgcgatg gtcggctata tctaattgcg cctaatttca
     1921 caagtgaggc tttgaaagct ttacttcaaa agctggatgc agacaaagac tttgccccta
     1981 acaaagttgt attttatggc agcaacttcg aaagtgcgaa acagatggaa ctcaatgaag
     2041 cactaaaaag ttatgctaat aaaaaatcta ttgatttaga tttggtggta aggaactaat
     2101 catgtctaaa gggttcacat tcgaaaaaaa tttacctcat caaaaggctg gcgttgattc
     2161 ggtaatgaat gtctttgtat ccgccatacc tcatcagacg gatcatgttg caattcgcct
     2221 gttagctaat ccagaactga atctatctga acaacaatac tacaacaaca taaaaaatgt
     2281 tcaggagttc aatggtattg agcactcaaa agataattac gatgctaaaa gcaacgtaat
     2341 tgatgtttct atggagacag gtacaggtaa aacttatact tacaccaaaa caatttttga
     2401 cctgaacaaa tcatttggta ttaacaaatt tatcatcatt gttcctaccc tatcgatcaa
     2461 agccggaacc gttaactttt taaaaagcga tgctataaaa gaacactttc gagatgatta
     2521 cgagcgtgaa ctaaagactt acgttgttga aagtcagaaa agttcgggta aaaatacaaa
     2581 atcgtatatg cctcaagcta tccatgattt tgttgaagca agtaatttca ataagaaata
     2641 tatacacatt cttgtgatca actcagggat gattaactct aagtctttaa ctgacactta
     2701 tgatgttggc ttgcttgata atcagtttga taccccattt tctgcactta gtgcgataaa
     2761 accattcatt atcattgatg agcctcataa atttcctact ggtaaaaaaa catgggaaca
     2821 tattcaaaaa tttaatgccc aatatatcat ccgctatggc gcaaccttta gtgaaggcta
     2881 taagaactta gtttatcgtc ttacagccgt agatgccttc aatgaagatc ttgttaaagg
     2941 tattgatgca tacattgagg atattgttgg tgatggtaat gccaacctca agttggtcaa
     3001 atctgacgga aaagaagcta catt
//
LOCUS       DAZGTJ010000220         2813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000220 DAZGTJ010000000
VERSION     DAZGTJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.222"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..971
                     /locus_tag="ACHK8E_005160"
     CDS             <1..971
                     /locus_tag="ACHK8E_005160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347260.1"
                     /translation="LFAPAAIKLAKSTAQWATILEKNAGAQMSQDQNAGGETRYIYNG
                     ISGSDVITVGKSLGGTGLNMTATRNDMKVMTGDGDDIIITGQDYGRLASAGQWDYKYL
                     TEMGNGNDTLIVGASNSNLNVIMFNDGSIAAVKKDGAQLGSVIPFDSAYDTADGGNIS
                     GTTIDMGSGNDTVLALGHENGGTAIINSTIKLGAGNDTIQINGDVKGGKSPSVITGDA
                     GMDTLIISNGSVYSEYFSGFENIELGSKGEVKIVAADLVGKDSNSIQGGMLKIIGNSD
                     SKVDLDGSDWIKGEIKNEGDITYNVYTHASAPNISVLIEDKITQVI"
     gene            1050..>2813
                     /locus_tag="ACHK8E_005161"
     CDS             1050..>2813
                     /locus_tag="ACHK8E_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347261.1"
                     /translation="MGMFNYKIYNAAHVTYLVSSSYKLAIYSNTGKVLGLGSFLASKL
                     FAKHPDKIDYSPDALNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGFTNIMARLSESLADGFYANSDYIAWASPLIFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLG
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGASGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKG
                     EKDFLFLSKDIVTSTDEFISCL"
BASE COUNT          806 a          437 c          736 g          834 t
ORIGIN      
        1 cattattcgc acctgcggca ataaaattgg ctaaatccac ggcccaatgg gcaacaatcc
       61 ttgagaagaa tgctggagct cagatgtctc aagatcagaa tgcaggggga gaaacacgat
      121 acatatataa cggtatttcc ggaagtgatg ttattacagt aggcaaaagc ttgggtggca
      181 caggcttaaa tatgacagcg acgcgtaatg acatgaaggt aatgactggt gatggcgatg
      241 atataattat cactggtcag gattatggtc gtctggcaag tgctggtcag tgggattata
      301 aatatctcac agagatgggg aatggaaacg atactcttat tgtcggggca tcaaactcta
      361 atctgaatgt aattatgttc aatgatggta gtattgctgc agttaaaaaa gatggtgccc
      421 aattagggag tgtaattcct tttgattctg cttatgacac agctgatgga ggtaatatat
      481 caggaactac aattgatatg ggaagtggta atgatactgt tttagccttg gggcatgaga
      541 atggtggaac tgctattatt aactcaacaa taaaacttgg agcaggtaat gacactatcc
      601 agatcaatgg ggatgttaag ggaggtaaat ctccttctgt catcactggt gatgctggta
      661 tggatacatt gattattagt aatggtagtg tatactctga atatttttct ggctttgaga
      721 atattgagtt aggtagtaag ggtgaagtaa aaatagtagc agcagatctt gtaggaaagg
      781 atagtaactc cattcaggga ggaatgttga aaatcatagg taattcagac tctaaggttg
      841 acttggatgg aagtgactgg attaaaggtg aaatcaagaa cgagggcgat ataacttaca
      901 atgtttatac tcatgcatct gcacctaata tcagcgtgtt aatcgaagat aagataactc
      961 aggttatttg atttgagcta aatagtgaca aggccgcgct gtatgcgtgg cctcttaata
     1021 tatagatttg gtaatttaaa ggagtctgta tggggatgtt taattataaa atttataatg
     1081 ctgcgcatgt aacatacctt gtttcttctt cttataagct tgctatatat agcaacacag
     1141 gaaaggttct tggattgggc agttttctgg catctaaact atttgcgaag catccagaca
     1201 aaattgatta ctctcctgat gctcttaatg aaggctggcg tgatattaca gtggaagaac
     1261 tcaggttatc ttcggaatat gtcgattcaa gaggttttta tcgctttccc agtccggtta
     1321 caggaaaccc tgcgtcagga tttggtcctc agttgaaggt tgttgggttg tttgatgctc
     1381 aacagaatct acagaaaatt gcaatatcat tctgtggaac aaatgatatc tgcgatattg
     1441 ttgattatac acagctcaat aatggaaaag cgctgtctga agcagaaaac ctgcttcaaa
     1501 gtatcagaga ctttgcaatc agtaatggtt taagtggtga agatgttatt gtcacaggct
     1561 atagcctggg gggcgggttt actaacatta tggccagact cagtgagtct ctggctgatg
     1621 gtttttatgc taattctgac tatatagcct gggcttcccc gctgatattt gacggggatg
     1681 ggaaggtatt aaatatcggt tttgaaaacg atccggtatt tcgtgtgctg ggagatgaat
     1741 ccgggttctg ggaggcagtt aaagcaatgg ggccgggctt gacaaccccg gatcgtgaat
     1801 atgactcttc tcctgataac attgttttat acacagacct ttatggttca ttcctctgga
     1861 atattatccc cttttctgtg cttgctctcc cgctgggaag ctggagtgcg cattttgcgg
     1921 gttcaaaaac tgacgccttt gaaagaattt tagggtctca gttttacgac tacatgtcac
     1981 cggacagtac agtcattgtg gatgagcaga gtggctggac atcctggtat tcatgggttc
     2041 atgacaaggg ggatgacagt cgtgaaagac ctgtattcat catcggtaat gatggcaata
     2101 atctgctggc aggtgggcag ggtggtgact atatagaggg ggcaggaggg aatgataaga
     2161 ttgaaccagg taagggagca aacagggttg atggtggcag tgggtatgac acgcttcttc
     2221 ttggcgatgt aatggatgac tggaatatat acagggtcag tgacgggaca ctgtttttcc
     2281 actcagaacg gggagccggt cttactgagg caacaggtat tgaaggtatc tctttttctg
     2341 gtgactggag gagtaaacta agcccttatc agatagaaga agagcagatt acagaccaca
     2401 ggtatcttct gaagtggcgt aataaaaatg ttgattacgg aaaacataca gaaggtacaa
     2461 acggagatga tagtctgttc ggaaatatta tttttggtct tgacggaaat gatatattac
     2521 atagtgccgg gggagaagga gtaagtcttt tacacggagg tcgcgggaat gattatcttc
     2581 atggggcttc tggtaatgat gagttgtacg gagcagaagg taacgactgg ttatatggtg
     2641 ggggaggtaa tgattatctg tatggtggta ctggtcatga tgtcttcgtc tttgatgctg
     2701 cttgtgaagg gaattcattt atcatggatt ttaataaaca taagggtgaa aaagattttc
     2761 tgtttttatc aaaggacatt gttacatcga cagatgagtt tatttcatgt ctg
//
LOCUS       DAZGTJ010000221         2813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000221 DAZGTJ010000000
VERSION     DAZGTJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.223"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..840
                     /locus_tag="ACHK8E_005162"
     CDS             <1..840
                     /locus_tag="ACHK8E_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0347262.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHK8E_005163"
     CDS             837..2381
                     /locus_tag="ACHK8E_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347263.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..>2813
                     /locus_tag="ACHK8E_005164"
     CDS             2378..>2813
                     /locus_tag="ACHK8E_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347264.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHG"
BASE COUNT          738 a          564 c          890 g          621 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtg
//
LOCUS       DAZGTJ010000222         2757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000222 DAZGTJ010000000
VERSION     DAZGTJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.224"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(737..2674)
                     /gene="iolC"
                     /locus_tag="ACHK8E_005165"
     CDS             complement(737..2674)
                     /gene="iolC"
                     /locus_tag="ACHK8E_005165"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFP0347265.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT          719 a          680 c          704 g          654 t
ORIGIN      
        1 ttaaagtatg ttgtagagaa aagtttgtct gaagtggcct actgaaatga attgcgcagg
       61 aaagtgagac ggaagatcgg tcagaccgac accagcaaaa tatgatatat ccgacgacca
      121 tcaaaggtca taaaggcgat aaggaatagg tgcgcagata tccgtcatgg ctctgcaaaa
      181 aactgtaaaa tgaagccgaa tgtacggggg cagtctgaac cccaactgaa gcatcttttt
      241 tgcacaaaat cgggagtcca tgtacggata taaagctgca acctaccgtc aatgtcagaa
      301 caatggaagc tgtgaaaaac gggatgcgtt aatataaact ccatgacagt agtggaacgg
      361 gttaatcaag caggattacc agcaagatga gggagctgat tcagcacagg cgacctgacc
      421 ctggccacgg ataaccgtct gtcgcccgtc tgaggtgtcg ttttttcgtt ctgccacccc
      481 tcggttgagt aaaatggaaa ttaaccgtca agctgcctgt agcgtaccga tcagaagatt
      541 gctcagatgg tacagctgtt gtacgttctg cggcaacgtg acgagggaac taccaatagc
      601 cagacagttc agaaatatca ttaatgcaga tacgaaacag cactgtcggg atgatgagga
      661 aagtaggacg gtcatgaaac atctttgaat acgccccgag caagaataca gacgcgggac
      721 gtaatgcaga cggatactaa agcgccggac gatattcgcg ccagtagtcg atcaatgtgc
      781 ggtatttttg tttcactgct gcgatgagct gttcatcgtt aatctggccg ctgaaccact
      841 gccgtgatgg ctcgccaaag atggtgcgac ctacggcgaa gcctttaacc catggtatat
      901 ccgcagcggc ggcaaagcca gatttcagct cgctttccgg tgcatccagt ccaagtatta
      961 aaataccgcg gcattcggga tcgtagcgct caatatgctg gctgatttct tgccaacgag
     1021 ccggacttaa cggtggaagc ttccaccagt caggctggat ccccagcagg taaaaacgtt
     1081 ccacaatctg tgtatagtaa tgctcgcttt gatctgctgc gtcgcgtggc aagatgacct
     1141 ccagcagcag atcgtgtcca gactggcagc aggcccggta tatctcatca atgagtgctt
     1201 cttgcttaag gcggagggat tctgcatcca gtgggtgata gaagacaagg catttcacga
     1261 cgtgctcttt cggccaactg acaagttgtg aaccgatatt accgtgctcc agcttcagag
     1321 gatatgaccc cggcatttcg ataggacgtc caatccacca tccctgacca gtcacgtcgt
     1381 taagtgcttt ctgaccaaaa gtagtatccg ccaggatgcc gcagttatgc agtaatccag
     1441 cctcgtgcgc cgcctgacgt gcgccttcca gtagtagcat cttcagctca gggatagtga
     1501 actcgctggc acctgtcgct ctggcgatat ccaccagttg attgcgatgg tcaaaggcga
     1561 atacacacag ttccggccag tgctgttttc gggttgtcgt tcggtgaaga tggttaaggt
     1621 gagggtcttt gtcgggtctg gtgatagcgt gctcacggga gagatagtca tccagttcct
     1681 gtttggttgg catcgcgggg gcgcagccgt ggcgcgatac aactaatgcg ccacatgcgt
     1741 tagcataacg acaagcttgc tcccaacctt catcgttcag atagcctcga agcagtcccg
     1801 acataaaggc gtctcctgca cccagcacat ttaggatgtc cacacgtaca ccgctgtgca
     1861 tctgcacctg agaccagtcg tcggcgatgt ttccttcaaa tactgaacac cctaatgcgc
     1921 cgcatttgca taccaatacc gcagaggtta gctgacgcac acggcgcagg gcggtcaacg
     1981 tatcagtact gccgccggcg atatgaaatt cctcttcggt gccaactatc agatcaaaat
     2041 ggtgcaatac ctgttgaagc tgttcagtca cctgtggtga agcgatgaaa cgtgtttcac
     2101 catcccccag cgatgtcaac ccccatagta ccggacgata atcaatatcc agcactctac
     2161 gcagaccgtg tttttgagca taggtcagcg ctttaagcac tgcggcgcgg gtattgatgt
     2221 gagaaagatg agtgccggtg acggccaggg cacgtgaaga ggcaatgaat gtttcgtgga
     2281 tatcctcagg cgccagcgcc atatccgcac agttatctcg ataaaagatc agcgggaagg
     2341 tgtcctgatc tttaatacca aggatcacca gggctgtcag acgttcagga tcagtcaaca
     2401 ggcattgtgt attgacgcca acacggttta attcttcgcg gatgaaacgt cccatatgtt
     2461 catcgcccac acgtgccagc atggcggaac gtagtccctg aattgctgtg ccgtaagcga
     2521 cgttgcctga tgagcctcct aaatattttg caaaggttgt catgtcttcc agacgtgacc
     2581 cgatttgttg accatataaa tcaacggcga cacgccccag acagataatg tccagcgttt
     2641 tttgctcact gctgactgtg ttgttcatag agatatcctc ctacagaaga ccgtgtgatt
     2701 tttgtttaca ctaaggttat ataatttcga tttcaacaaa aaggaaaaaa taattcc
//
LOCUS       DAZGTJ010000223         2689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000223 DAZGTJ010000000
VERSION     DAZGTJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.225"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            311..994
                     /locus_tag="ACHK8E_005166"
     CDS             311..994
                     /locus_tag="ACHK8E_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0347266.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            1038..>2689
                     /locus_tag="ACHK8E_005167"
     CDS             1038..>2689
                     /locus_tag="ACHK8E_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP0347267.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKIT"
BASE COUNT          793 a          589 c          651 g          656 t
ORIGIN      
        1 ttttctatca gcatcgcaaa cttcgcaata aaacatcact caactgaata tttacctttc
       61 cctgccctgg gcctttatcc ggtgaaacat ttgacattga ttgacaccga tcattaaaat
      121 ccgcgcgatt aatattattg gctacaaacc gaaattgttt gacacttaat aacatattcg
      181 cacaaccgat taatacaaaa attctctaac aaatttattt gtcggatatg ctccactcta
      241 atcattcata aaaaaaatga ttgtgccgta attatcaact gtatcgggat tatgaaaagg
      301 aattcatcct atgaacctaa agaaaacact gttaagcgtg ttaataatag tgcctctctg
      361 cttattggca gggtgtgact atattgaaaa agcgagtaag gtcgacgacc tcgttacaca
      421 gcaagagttg caaaaaagca aaattgaggc gcttgagaaa caacaggaac tcgataagcg
      481 aaagatagaa cattttgaaa aacaacaaac tactatcata aatagtacca agacgctcgc
      541 tggcgtggtg aaggcggtta aagacaaaca ggacgaattt gtctttacag aatttaaccc
      601 ggcacaaacc caatacttca ttttaaataa cggctctgtt ggtttggcag gccgggtgct
      661 ggctattgat gcagttgaaa atggcagcgt tattcgtatt tcgctggtta atttattaag
      721 cgttcccgta tcaaatattg gctttcatgc gacatggggc aatgaaaggc ccaccgatgc
      781 caaagctctg gcaaagtggc aacaattact tttcaacacc acactgaatt ccactctgca
      841 attaatgcct ggtcaatggc aagacattaa tctgacatta aaaggcgttt cgcctaataa
      901 cctgaagtat ctgaagttgt ccatcaatat ggccaatctc cagttcgaca ctgttcaatc
      961 tgctgaaact cggcagcgaa aaaacaaaaa ataaatgcag cttctgcgct tatatcaagg
     1021 aatagagagc atcaataatg aacaaaattt ttaaagttat ctggaatcca gcgacgggca
     1081 gttacagcgt tgccagcgaa acggcgaaga gccgtggtaa aaaaagcggg cgcagtaagc
     1141 tgttaatttc tgtactagtt gcgagtgggt tgttgtcgtc gtttggagcg ttagctaata
     1201 actacgacgg gcagccaact gattatggcg atggctcagc aggtgacggc tgggttgcta
     1261 tcggtaaagg ggcaaaagca aataccttta tgaacactag tggcgcgagt acagctttag
     1321 gatatgacgc gatagccgaa ggtgagtaca gttctgccat cgggtcaaaa acccttgcaa
     1381 ctggtggagc atccatggcg ttcggggtta gtgcaagagc aatgggtgac agaagtgtcg
     1441 cgctgggtgc atcgtcagta gcaaatggcg atcgttcgat ggcttttggt cgttacgcaa
     1501 agacgaatgg ttatacatct cttgctattg gggactcctc ccttgccgat ggtgaaaaaa
     1561 ctattgcgtt aggaaatacg gctaaagctt acgaaattat gagcatcgcc ctcggtgatc
     1621 atgccaatgc gtcaaaagaa tattcaatgg cgctgggtgc aagtagtgcc gcatctgcgg
     1681 ctaacgcgat tgcagtaggc cgcaacagtg ctgcagctgg cgtagacagc ctcgcattcg
     1741 gtagactatc cgccgccaac gccgcaaacg cgattgctat gggtgcagag agcaaagctg
     1801 ctgaaaatgc gacggcagtt ggtactaacg cggaggcaaa tggacttaat agtatcgcgt
     1861 tgggctccgg cagtattgca gatgtcgaca acactattgc tctgggcaat caaagtcagg
     1921 cagtagcggc tggcgcaatt gccatcggtc aagggaataa agctgacggc gcaaatgcta
     1981 tcgcgctggg taatggtagt attacaggtg gtgtaaatgc tattgctctt ggacaaggca
     2041 gttatgccgg tttagaaaat ggcactgcaa ttggtgctca agccagtgct caggggaaaa
     2101 attcagttgc tctgggtgct ggttctgtag cgactgacgc ggatactgtt tctgtgggta
     2161 acacaacagc tcagcgacaa attgtcaata tggcagcagg tgatatcagc actaccagta
     2221 ctgatgccat caatggatca cagctttatg ctatcagtaa gtcagtagcg gacaatcttg
     2281 gtgggggggc taccgtcaat gcgcaaggcg tcgttacttc cccaaattac aggctgaaaa
     2341 gtggtatttt cggcactgtt ggcgacgcct taacgggcct ggacaataat acgttacaat
     2401 gggactccct taaaaaggca tatagtgcgg cacatggtac agatactacc agtaccatca
     2461 ccaacgttaa agacggcgct atttctgata ccagtaagga tgcggttaac ggttctcagc
     2521 tgaaaaccac caacgataac gtagcgacca atactgccaa tatcaccacc aacactaaca
     2581 gtatcaatac cctgacggat tccgttggcg accttaaaga cgatgccctg ctgtggaatg
     2641 gcaccgcgtt cagcgccgcg cacggcaccg aagccaccag caaaatcac
//
LOCUS       DAZGTJ010000224         2670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000224 DAZGTJ010000000
VERSION     DAZGTJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.226"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..2669)
                     /gene="chiA"
                     /locus_tag="ACHK8E_005168"
     CDS             complement(<1..2669)
                     /gene="chiA"
                     /locus_tag="ACHK8E_005168"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFP0347268.1"
                     /translation="MIGMGLVCSALPALAMEVWNNQQGGNKYQVIFDGKIYENAWWVS
                     STNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPAN
                     GGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDA
                     SNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNY
                     AVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEI
                     SQYGNPGSCSVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADSSNDYSLQAW
                     SGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTATAAEMSQYGN
                     PTTCEIDNGGVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKY
                     EPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKA
                     QSYSNEELNNAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNPWRVFVDWTG
                     TKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLP
                     TWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVD
                     NLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTP
                     EGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLK
                     GLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIAR
                     DLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFT"
BASE COUNT          618 a          712 c          637 g          703 t
ORIGIN      
        1 ttagtaaacg gcgcgaagat ttcgctaaat gcgtacttcg gtgcctgttc tttagtcagg
       61 ccgtggaatt ccggagacag gttagtgccg cctggcaggt cgcgcgcgat tgaccagata
      121 ccaaccatac cgagattacg cttctgcgca tcctgcatga ccagacgagc atcagagaga
      181 tagaacacct cgccctgaac gtcgttcacg ccaaccatcg gcgtggtacc catcatagcg
      241 tcaatttctg catcgctctt attgggatgg aggcctttca attgtgaatg caggttggca
      301 atcgcagacg ttgcacactt accgtgaatg ttctggcctt cggtatttgc agactgacaa
      361 atcgcgttac cgtagtccat tgtcatcacg ttcacacccg ccagctcaac acctttcgct
      421 ttggcatcgc tcaggacatt catcccttcc ggcgtcaggc cagtcggcag aattggcaag
      481 gtgtaccaga tagcaatatc tttgccctct gacttccatt tatcctgcac tttcttcaca
      541 gcaaggttac gacgttcaat agatgcctga tccgcaaccc aggtgccttc gatatcgaag
      601 tccaggactc tgaggttcag gttatcaacg atgtcataat aatgctgcat cagatcgtct
      661 acgttcttac aggaagcagc cagcggagcg ttgttagcac caccgataga cagcatcaca
      721 tcgccgccag cttcacgcag agctttgatt ttgctgtact gagcgtaatt ctgcataccg
      781 taagcggtac cccatgtcgg cagacaggtg ttcgcatctt tactcaccac aaacgccagc
      841 gtgaagtggt tgacgttatg attcttagcc agcgcagcca gatccgggat cgtattcagc
      901 gtaaagtcaa catacggtgc ataaacgtgt ttcggccacg ctttcttcgg cgtacctaca
      961 cgttctttgg ttccggtcca gtcaacaaaa acacgccacg ggttgctgtc agaaggagaa
     1021 actttctgca ctttactctg agaaatgtag ttcgcaccgt taaagcgaat cagcgtatcg
     1081 ctggcataaa gcgtttctgg attaaactgc ggcgcattat tcagctcttc gttgctatag
     1141 ctctgagcct tacctaacgg cttccacggg cggctattgc taccggtggc gttttggttc
     1201 gcaaccagag ctgggttgtc gctttgcgtc cagaacagcg cttcataagc ctgcccatca
     1261 acaacaacgc gatcaccttt cacgtacacg gtggaagcgg accatgcttt cgccggttcg
     1321 tatttcttcc acgggttatc gccgcccggg acaaagcccc atgcgtcttg atcgacagag
     1381 gttttataat gtgcatcgtt ataatctacg atgctgttcg cgctgtacgc tttgctggcc
     1441 tggaaaccat ccgcaacaat gacgccgcca ttatcaattt cacaggtagt tgggttaccg
     1501 tactgactca tttccgcagc ggtagctgta cgcacgagac gccatgggtt attggagttt
     1561 tctgcactag tgccacgtgg gcaatcttca gaaccaaccc accaggcatt ctgataaaca
     1621 ttaccattga agataacatg gtaaatttcg ctaccttcct ggccactcca cgcctgcagt
     1681 gagtaatcgt tactgctatc cgctggtgtt gatggctcgt tattatccgg agtaggagta
     1741 actggagtta ccggtgtttc cggagttgga tcaaccggag tcacagcacc gctattatta
     1801 tccggcttaa cggaacagga acccggatta ccatactggc tgatttctgt tgccgttgcc
     1861 gcgcgaacat aacgccatgg gttgctggca tcgttttctt tcgcatcgcc tgggcaattt
     1921 gccgcgccta cccaccaggc gttcttataa atgccgccgt tgtagataac gtaccatgta
     1981 gaagaacctt cctgaccttt ccaggctacg acagcatagt tgtcgtttga cttatcagct
     2041 ggagctggct gaacatcagg agccggttta acgtccggtg ccggtttcac atccggcgca
     2101 ggctgtggtg ctgaagtaca ggactgtgga ttactggttt ctgagatttc cgttgccgta
     2161 gcggcacgaa cataacgcca tgggttgctg gcatcattgc ctttcgcatc acccgggcaa
     2221 ttagaagagg ctacccacca ggcattttta tataccgcac cattaaagac gacataccag
     2281 gtctgaccgc cctgctgttt attccaggca actacagaag aattagacgg aacagtgccc
     2341 tgtgctggtg tagccgaacc gccattagcc ggcgtattgg atgcaggcgt atttgaattt
     2401 gaagaagatg agctgccgct cttttcgcag gaaagtgtat tgccaaactg actaatttca
     2461 gcagctgtcg cggtacgctt taaacgccat gggttagttg catcatttgc ttttgctttt
     2521 cccgggcaat ttgtagaaga aacccaccag gcattttcat aaattttgcc atcgaaaata
     2581 acctgatatt tattaccacc ttgttggtta ttccatactt ccattgccaa tgctggcaga
     2641 gcggaacaca ccagccccat accaatcata
//
LOCUS       DAZGTJ010000225         2655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000225 DAZGTJ010000000
VERSION     DAZGTJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.227"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(149..910)
                     /locus_tag="ACHK8E_005169"
     CDS             complement(149..910)
                     /locus_tag="ACHK8E_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP0347269.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1700..>2655
                     /gene="subA"
                     /locus_tag="ACHK8E_005170"
     CDS             1700..>2655
                     /gene="subA"
                     /locus_tag="ACHK8E_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP0347270.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKMSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          760 a          606 c          604 g          685 t
ORIGIN      
        1 caacattatt atgaagagaa taaaataata agaaaaacag aaatatcctg taaagcagat
       61 aacagacaac cctgcagtat aattacggca gggagtttcc gtttaattac agaagacagg
      121 taattattat ctttcaggag tagtttcgtc agaagttata agtcacacca agcatgatgt
      181 catgactttt aacatcaact tctgatttat atttatcgcc ccactcatcc ttgtaactca
      241 cactggcatc acctgcatca agatagcgat agctgaggtc cagagcgata tccggggtta
      301 cgtcatagcg gacacctgcc ccaaaactcc atgcaaaatt gttatcatat cccgggtaat
      361 gcaacgcaga aaaattgctc acctcaccag atttattcca ggaggtatca gtatatgttg
      421 ccttatgatg tatccgtgca taacccagtc caacagacac ccatggagtg aatgcactgc
      481 tggtgaatgc actgctgttt ctgaagtcat aatacgtatt caccatcaga gtattcacac
      541 tgagcctgtt ctgtgtgtct tcccgaccac caccgaccat cggagagtac catgtatcca
      601 gtgtataacg ggagtccgca gctccgcggc catagaattc cacttccgtg cgcacaggaa
      661 cattgtaatg ctgataaaaa tcatagccca ccgcaaggcc ggcaccgaat accgtatcgt
      721 tcttatcgcc gcccttatac ttataaacat cgtccccaaa gtcctgacga aagcgctggt
      781 cagtctgagt cacaaccgaa gcaccagctt taccggtcat ataaaagcct gttttgcctc
      841 tatcagccag ggccaggcct gaaaacatgc ccaccacagc aagcgctgaa accgcaataa
      901 tctttttcat ttcagttatc tccgctatca ctttccccga acgataaacg tggttgctta
      961 ttctaaaccc atgcaaaaga cagggtaaaa agagaaaaat cgatcgaatc gatcgacaaa
     1021 atcgatcatg catgatgaag atttcttatc gaatacataa aaatagtgat ggcaatcgtg
     1081 gcaggagctt acgtcaggcc ctgaagtact gacacaggca ttgatggcag gtgatataag
     1141 gagtgctcca gggaatgact cccgactgac ccaggcactg tcccgttgtc aacccgtatg
     1201 gttactgctc tgactcattt cctgtcgtca gacgttcaac tctggaccac tggttatgac
     1261 gacaggtgtc gctcatttgt cagcactata acggtcaaag atcgtggcat tgacacattc
     1321 tccagaggta aatttctcaa gaggtaacaa ctctgccagg aggatatctg cagagagtta
     1381 cattcgtttg ggttcatatc tgtgccgcgc gttttccttc catcagtgaa caaacattca
     1441 gtcccatgac gaccgggcaa atagcgtgtt cacagatatt gctgtccagg tacgaagtac
     1501 cataatggat attggtaagt tattgggtga cccccctcca tgaaaaaacc aaatataaaa
     1561 atgaatgttt cgggagagac ttcactcgct ttattgatag tgattgttta tcttttactg
     1621 aagacaggat atttcagatg tgatatccct gtaacatatt gaccagcaat actttttatt
     1681 tgtaccactg gaggatattg tgctgaaaaa tttacggctg catattctgt tcctgctttt
     1741 tatctcagtg tcagtcaggg cggaaaaacc atggtatttt gatgcaattg gtctgacaga
     1801 aacaaccatg tcgctcacag acaaaaatac cccggttgtc gtatctgttg ttgattccgg
     1861 agtggcattt gtaggaggcc tcagcgacag cgaatttgcc aaattcagtt ttactcagga
     1921 tggttcgcca ttcccggtaa aagagcctga agctttatat attcatggta ctgccatggc
     1981 ttcactcatt gcctcacgtc atgaggttta tggtgtttat cctcatgcac tgatatccag
     2041 cagaagggtt attcctgacg gtgtacagga ctcatggatt agagcaactg aaagcattat
     2101 gtcaaatgtt tttcttgcac caggagaaga gaaaatcatt aatatatcgg gaggccagaa
     2161 ggggatatct tccgcatcgg tctggtccga actgctttcc cgtatgggcc ggaataatga
     2221 gcgattaatt gttgcggcag tgggtaatga tggcgcagat atacgaaaaa tgagtgctca
     2281 gcagagaata tggcctgcgg cttaccatcc ggtcagcagt gtgaataaaa agcaggatcc
     2341 tgtgataaga gtggcagctc tggcacagta ccggaaagga gaaacacctg tattgcatgg
     2401 tggaggggtc accggaagtc ggttcggaaa cggctgggtt gatattgccg caccgggaca
     2461 gaatattaca ttcctcagac cagatggaaa aacggggatc ggtagtggaa cctcggaagc
     2521 aacagctata gtttccggcg ttctggcagc gatggtctca tgtaatcccc gtgcaacagc
     2581 gacagaactg aagcgtacac tgctggagtc tgcagacaaa tacccctcac ttgcagataa
     2641 ggtgacggaa gggag
//
LOCUS       DAZGTJ010000226         2530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000226 DAZGTJ010000000
VERSION     DAZGTJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.228"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            15..320
                     /locus_tag="ACHK8E_005171"
     CDS             15..320
                     /locus_tag="ACHK8E_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347271.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            375..1739
                     /locus_tag="ACHK8E_005172"
     CDS             375..1739
                     /locus_tag="ACHK8E_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP0347272.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PRVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELK
                     TEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMENAGKP
                     KSQP"
     gene            1755..2387
                     /locus_tag="ACHK8E_005173"
     CDS             1755..2387
                     /locus_tag="ACHK8E_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP0347273.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          672 a          603 c          701 g          554 t
ORIGIN      
        1 acgagggctg aatgatgaac ccgttattta atgacatcca gatgcgcctt ttttatctga
       61 atcatgctcc gtattcctgg cactggaatg tcagattcag gccacaggaa gccgtatata
      121 ttggtaatga tgcatgccat attaccatta cctgtaatca gtccggtttt catttaacca
      181 gggacgggca gcgactgttc actgaacgtt atatcagaac actcagtgag ttattagcgg
      241 ttctgaagcg gcggtgggat gtgacaccgg caatcatccg tgcggtggaa tacctttccc
      301 gggtaccggt atcacactga tgtcctttcc gtaactgtta atcatgattt aatacagcaa
      361 caccgaatct gccgatgact tctgaagtga aactgaaaac cggtggtgac ccccggtcat
      421 taccggatta tgctgccctg cgtgatgaaa tcagcaaact gacccacccg gcacgtccgg
      481 atgtggactg gcgttacgtg gaaacgctct gcctgcgtct gtatgaacac aatggcgtgg
      541 aattacagac ggtatcctgg tacaccattg cccggatgca tacagcaggg ttaagtggtc
      601 tgaatgaagg gctggctctg attgtggcgc tgacccggca tcactggtca gtgatgtggc
      661 cgctgaatac ccacgcccgg ctggaaatta ttaccggtct gttcagtcgt ctgcaaaaaa
      721 cgctccgggc catgccaccg gatgagcgtg acaacctgcc cctgctgtat cagacagaaa
      781 catgcctgaa aacgctgagt gacatactgg cccggcatga acttaaacag agcagtaaag
      841 tggctttacc ggaagccatg gtgaagggat atatcacccg tctggagaat cagcctgtgc
      901 agggtgaagt atcgtcgccg gtaacccttc cggctcaggc actccggtct gatgctccgg
      961 atgtacagga acatcagccc ctgccgtact cccgtctggt gtatgttgtt tctgaaacaa
     1021 aaacagagcc cacactcttc cttcaaaaat ccccgctcac gtttttgaaa acgtttgtta
     1081 caggtgtctg tacggcattg ctggcggtct gcgtggccat tccgggatgg cagtttctga
     1141 cacagccatc accggcggag cagcaactca gggcgctgct ggcaccacta ccggatgtgc
     1201 tttcagcaga ccagatggcg caactcagca gaacaccctc actgctaaat catacgtcag
     1261 agtggataac cctttccggt cagcagataa cgcgactgac agaactaccg ccggcataca
     1321 acctgcaacg cagtgcgcag ctgctgcagc aactgagggg acttttcccc gacaatcccc
     1381 gtgttcagga aatggtggat aactggcata agagcgtaag gagccgcgcg ctcccggaag
     1441 aggcgatgac gggctggaac gaaggcatgg cccgtttaca gcaactggct gagcgcctga
     1501 accgtctgga tgaacagcgg ggaaaataca tgacggtcag tgaactgaaa acggaggtgt
     1561 ttggcatcat gcaggctttt aaccggcaca taccggcaga agagcagtta cgtcgttatg
     1621 atgaagcccg taaccagaat ggcagtgaac agcagcaaaa acaggctgaa atggcgctga
     1681 atcagttgat taatcgttat cagatggaga acgcaggcaa accgaagagc cagccataat
     1741 ggctgagata aaacatggaa atggaaaata cccccggaag agcgcgcaca ccatggacaa
     1801 tcgaagatgt tcgctacctt gaaacgcatt atggcgtgaa gaaaacccgc gaaatagcgg
     1861 catacctgaa cagaagtgcg acagcagttc gtgccatggc gtatctggtg gggataacaa
     1921 caccagtggc tgcggactgg tcagagacag agattgagat tattcgcagg gactatccgg
     1981 caggcgttgc gcttgctgat atttgtgccc gtctgaacgg acgagcgccg aaatccgtgt
     2041 gctggatggc ccgcaggttt ggtctgaaac gcctgggtta ctggagtgca gaagaagatg
     2101 aaatcctgcg gcaatattat ccttcagaag gtgtgaaagt gagccgacgc ctttccggac
     2161 gtacagaaaa tgcagtgaaa ctgagagcgc gggagctggg tgtaaagttc acgggaaacg
     2221 ggcgattacg tctctggacg gcagaggagt ggaatgtgtt gtccggaaat atggcatcgg
     2281 ggctatcggc actgatgaag ttactgccgg ggcgttcccg tagttcagtt cagggggcta
     2341 tgaggcgttt acagaaaatg agtgcgcaga aatctgtcag gcagtagcca aaatattcct
     2401 gcatcaggag aacggcagtg gcaataataa gcagcaaact gatacataat gcagcttctg
     2461 taaacggtaa tgatcaagta gggatatttt tatgttgact ggttgtgaaa aaatgatcaa
     2521 aatttaaatt
//
LOCUS       DAZGTJ010000227         2510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000227 DAZGTJ010000000
VERSION     DAZGTJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.229"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..534
                     /locus_tag="ACHK8E_005174"
     CDS             <1..534
                     /locus_tag="ACHK8E_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0347274.1"
                     /translation="IGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            531..1100
                     /locus_tag="ACHK8E_005175"
     CDS             531..1100
                     /locus_tag="ACHK8E_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347275.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKVQHELLRSEEK"
     gene            1344..1571
                     /locus_tag="ACHK8E_005176"
     CDS             1344..1571
                     /locus_tag="ACHK8E_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFP0347276.1"
                     /translation="MEILVNSREMALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWK
                     IEGKQPKTLGGNFTILIAKKDGQVLNVIHTK"
     gene            2123..>2510
                     /locus_tag="ACHK8E_005177"
     CDS             2123..>2510
                     /locus_tag="ACHK8E_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFP0347277.1"
                     /translation="MFDRIYLGDRAIKKIEFYLWTKQIRIQVNLISRIAYGTTEWNFY
                     DNEDLEDGYLVFSDVSFFNITPNGSIPDDYIISIITDNVNVEYFESTIVAVGQIPNTN
                     SGDIDNIGECQIFIRYKHSWIENKFKE"
BASE COUNT          877 a          414 c          506 g          713 t
ORIGIN      
        1 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
       61 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      121 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      181 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      241 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      301 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      361 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      421 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      481 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      541 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      601 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      661 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      721 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      781 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      841 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      901 taagagcggg tatctatcaa aaaaagagca gtgatccagc tcagttgttg gcagtccttg
      961 ataattcaat catggttaaa tattttaaag atgagaagcc aacttatgct cgaatgacag
     1021 cacatctccc gaacaaagat gagtcttacg aatgtttaac aaaagtacag catgaactgc
     1081 ttcgtagtga agaaaaataa aatctcttcc tgagaaaata aattaactat tcgaaaaaat
     1141 atgttcttaa tgggtatcca ggtacaccag aaggtcgatt tggaaaaatg gattccgatg
     1201 gtaagaatat catatattct gaatgtaatg ttttacccac aacttttgag attcgatagg
     1261 tgtagagcat gagaaactca ctaatattac ttattatgat attgtttaca atatcacact
     1321 cagtatttgg aaaagaaaat tcagtggaaa ttttagtaaa tagtcgtgaa atggctttgg
     1381 aattatcata tgtttacatt aaatatgtat acgggaaaga aaaagcagaa tttcagaaac
     1441 catatagtat tacagatgac aataattgtt ggaaaatcga gggaaaacaa ccgaagacgt
     1501 taggaggaaa ttttacaata ctaatagcga aaaaagatgg gcaagtgttg aatgtaatac
     1561 acacaaaata atccactgta cagtcgattc aaatatttta ccaaggtgtg ttttgtaaca
     1621 attttgtgta caatatgact ctacatatcg acgaaaaaac cacttgattt gacaggaaca
     1681 cattcaaaaa aatttcttta ttcatttcag gagcgatttt catctgttac ttgaagtatc
     1741 tgtgagatta agtgactccc cctaacgtcg tactaaatag taattactgt ccataccggg
     1801 agcagtattc ggcgctcccc acatgcagca gaataaccgc atcagcgaag atgtattgta
     1861 tttttactat cacaatgtgt acggacggtt tatggaaaag aatgaaaatt agatacgcga
     1921 tggggcggca ggttacacgg ttatcactac gatagccaat actggctggt gcattaccgc
     1981 cgtgaacaat aggacgccgt actgctggaa atttcttacc tttatgactc cccgcgccgc
     2041 cgtattggga aacgggaagc aatcaagatg aggctgatat aatgtgcgga atgcctgagt
     2101 atttttagaa taggggggta atatgtttga tagaatatat cttggtgatc gagcaataaa
     2161 aaaaattgaa ttttatttat ggactaaaca aattagaatt caggttaatt taatctcaag
     2221 gatagcctat ggtacaacag aatggaattt ttatgataat gaagacttag aagatggtta
     2281 tttggtattt tcagatgtta gtttcttcaa tataactcct aatggctcta ttcctgatga
     2341 ttatataatt tcaataatta cagataatgt caatgttgaa tattttgaat caactatagt
     2401 tgcagttgga caaattccaa atacaaattc tggagacatt gataatattg gtgagtgtca
     2461 aattttcatc aggtataaac acagctggat tgagaataaa ttcaaagaaa
//
LOCUS       DAZGTJ010000228         2493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000228 DAZGTJ010000000
VERSION     DAZGTJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.230"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(87..500)
                     /gene="hns"
                     /locus_tag="ACHK8E_005178"
     CDS             complement(87..500)
                     /gene="hns"
                     /locus_tag="ACHK8E_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP0347278.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1104..1721
                     /gene="tdk"
                     /locus_tag="ACHK8E_005179"
     CDS             1104..1721
                     /gene="tdk"
                     /locus_tag="ACHK8E_005179"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP0347279.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(2004..>2493)
                     /locus_tag="ACHK8E_005180"
     CDS             complement(2004..>2493)
                     /locus_tag="ACHK8E_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0347280.1"
                     /translation="RKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTM
                     QLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM
                     RMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRESAFPRVVKERPWKYPTAPKKSQS
                     VA"
BASE COUNT          640 a          551 c          553 g          749 t
ORIGIN      
        1 gcttattctt attaaattgt cttaaaccgg acaataaaaa atcccgccga tggcgggatt
       61 tttaagcaag tgcaatctac aaaagattat tgcttgatca ggaaatcgtc gagggattta
      121 ccttgctcat ccattgcttt tttgattaca gctggagtac ggccctggcc agtccaggtt
      181 ttagtttcgc cgttttcgtc aacgtagcta tattttgccg gacgctgagc acgtttagct
      241 ttggtgccag atttaacggc agcaaggcta ttcagcagtt cgttcgggtc aataccgtca
      301 gcgatcagca tttcgcgata ttgctgcagt ttacgagtgc gctcttcaac ttcagcagca
      361 gccgcgcttt cttcttcgcg acgttcgtta acaacaactt ctaatttttc cagcatttct
      421 tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa gagtacggat gttgttcaga
      481 attttaagtg cttcgctcat tgtagtaatc tcaaacttat attggggtgg tttgttgagg
      541 tgataataga gccttaaatt cagttgtgca atagccagga atgtaaggaa ttcaaaattg
      601 ttctttattt tgtgccgcca ataaatatct tttcataaaa ttagccagaa aagacgcggc
      661 atatagccct atttgcgccg atgatttcgc agcacgtgag gttaaaactt cctgattcat
      721 gtcacatttt atggggagat tatcgtaggc tgacgacctt tcagtcttct gtattagtcg
      781 tgtttacaag aattccctat taagcgaatg atgaaaagta gaacagtcgc aataagagca
      841 gggacttagt attgcactat ctcctggagg tcaacagagg gctattactt gcgcaacagg
      901 ttaaagattg tgaatagtta ccagcagtca tttacccgct tataacaagc gaggcagttg
      961 taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg agaacgcata taccacttct
     1021 gtgcatactg ttgagctgaa aaactgacga attatgataa actccagcca actttaattc
     1081 atatcattga gggcctgtgg ctgatggcac agctatattt ctactattcc gcaatgaatg
     1141 cgggtaagtc tacagcattg ttgcaatctt catacaatta ccaggaacgc ggcatgcgca
     1201 ctgtcgtata tacggcagaa attgatgatc gctttggtgc cgggaaagtc agctcgcgta
     1261 taggtttgtc atcgcctgca aaattattta accaaaattc atcattattt gatgagattc
     1321 gtgcggaaca tgaacagcag gcaattcatt gcgtactggt tgatgaatgt cagtttttaa
     1381 ccagacaaca agtatatgaa ttatcggagg ttgtcgatca actcgatata cccgtacttt
     1441 gttatggttt acgtaccgat tttcgaggtg aattatttat tggcagccaa tacttactgg
     1501 catggtccga caaactggtt gaattaaaaa ccatctgttt ttgtggccgc aaagcaagca
     1561 tggtgctgcg tcttgatcaa gcaggcagac cttataacga aggtgagcag gtggttattg
     1621 gtggtaatga acgctacgtt tctgtatgcc gtaaacacta taaagaagcg ttagaagtcg
     1681 gctcattaac ggctattcag gaaaggcatc gccacgatta ataagaattt ctttactgac
     1741 agggtgagca gggcgctttt atcctgtcag ttcgttttac tcactttatc cggtgtatat
     1801 acccttctcg acagttcttt aacgccgcta tacccctcac agagcgcttt tacagtgacg
     1861 ttgacttgtg acctgtaaag tacaatatcg ctgtgtgtag gcgttataca tcgccgtaaa
     1921 tatgatgcag gcaaatgctg tcggtttatg ggaaagttgc tttgggtaaa aaaaacggcc
     1981 ccagaaggga taatgccagt cagttaagca actgactggc tctttttcgg ggctgtgggg
     2041 tatttccagg gcctctcctt taccactctc gggaaggcac tttcccttct tgtcggtaat
     2101 ttcacaagtt gtcccatact tgcaagatcg cgcatcagct ccggtatacg tcccggtgaa
     2161 gcgccctgca atgtcatcag cattctcatc accattccgc atgattctga gaaactcagt
     2221 tgattcggcc agtaaccttt cagatgttcc gccattttaa tcatctgata tctcaccaga
     2281 ttataagcca gtaagacacc ccacagctct tgctccacaa gctccggctt tttacttctc
     2341 agcgtcagcc tgctcagttg catcgtctgt tttatctccc tgtatcccag ttcgatttcc
     2401 cagcgatgac tgtacagatc cgccatttct cctccgggga agcgcatggc gtccgtcatc
     2461 gacgtcagca gatggcagac ttttcctttg cgc
//
LOCUS       DAZGTJ010000229         2484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000229 DAZGTJ010000000
VERSION     DAZGTJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.231"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(104..1393)
                     /locus_tag="ACHK8E_005181"
     CDS             complement(104..1393)
                     /locus_tag="ACHK8E_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP0347281.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIGRLAISAWGTLATILGTGTAVLAVG"
     gene            complement(1393..>2484)
                     /locus_tag="ACHK8E_005182"
     CDS             complement(1393..>2484)
                     /locus_tag="ACHK8E_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0347282.1"
                     /translation="FANSKLRMEDRRGEEHIKLATEYGKTQLNSGHLVDSQGQLRGKG
                     TELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLNQQLQQLENAAERA
                     QALKADIDSQIQMFAQRLKPLNEAVLFSAPEGIALTSGEDMQMSAARNVAVNAGGDIS
                     TGTMGNLTALAGERLGLFARTGQLSVKSGEGPVEVQAQNGSMRLFAEKKLTISSESDI
                     SFTGKKRITLIGGGSYLKLEAGKVEYGTMATCMRRVKRTMTAAAQSMPMAFPRMSSSH
                     IYSAIYRLLDKHGNILVNTPCRLTTPSGQTVTGYSDNEGRSVPVYTRQQEDVELHFFV
                     KKPQPEETMWFVGETDTQQLETELRESLP"
BASE COUNT          493 a          760 c          606 g          625 t
ORIGIN      
        1 gattgactgc cggactgccg tctttgtatt tcacggaggc ttccggcagc catttctcaa
       61 tgtagttcag ctcaaaatca tgcaattcca tacctgctcc tgtttaacca actgccagta
      121 ccgccgtacc cgtaccgaga atcgtcgcca gtgttcccca ggcgcttatt gccagacgac
      181 caatcagtaa aaacgcgcca ataatggctt ccgggatacc cagcgttacg accagtccgc
      241 cgacacccac agcaaccaga ataacgccga ccgttttcgc gacactggtt aaaacctgta
      301 cccagtccac ctgtttcagc atatcaagcg tcatatcggt ggcgtgctgt atttctgtcc
      361 atgcggcctt aatctgggtg ttggtccagg atttcaccga cttaccggtt tggctggccc
      421 agacccagac ctcacttccc tgccggtaaa cccactcacc gctgtcgtta agccagctgg
      481 ctgctgaatt caggaccagt tgcgtctctt ttgacagggc attccagcct tcagccacac
      541 cacgtaccac accctcactg agatcagata ctgctttacc ggcgtaagtc cagggctcta
      601 ttctggcagc aacgggggtt gatggcggac ggggcataaa gaccggcggg tagagagcca
      661 gcggccagta tttcgcgccg ctggtctgcc aggctttcat cgtcacgttg atcacctgat
      721 caacccactg gtgctcttcc gggcgacagt cgtggatctc cagaatggtc atgcgatcct
      781 catgaccaat tccaccgaca atctgcatat actcttcata ttggtcttca tacagcctat
      841 ctcccggaaa cttcacctcc accaggcggg ccaggttatc gctgtgcata ttgccttcgg
      901 tatccggccc cgccagtccg ggccagcggt cctccttatt tttaacaata atcacgtccg
      961 ggcggcggta aatgcccctg ccgggcacag ggaaaggatt ggtcgtgtgg cgacggccag
     1021 caccgtcttt ttccagcagc gtactgctca gaaaagggcg tggtggcgtg gtgcgcatat
     1081 caaacgccac ctccgcctta taataccagc agtaattgcg ggcgatttca tccacacgaa
     1141 taagaccact cataacggtc tgcttaaggc tcatctggta agttttacct ttccggcgga
     1201 tttgtgccga cagttgcgga aaacgcatgg cgtattccac ctttatatgc aggtactgac
     1261 ggttttccgt ggttggcaga cgcgactgct tttcgaccat ttcgacaccc gtcgtagtgc
     1321 agtcccacgg ctggttgttc gccccggtgg gaatatcaga tgtcggtgtc tcgggtaaat
     1381 cgggcatttc cattatggca ggctctccct gagttcggtt tccagttgct gcgtatcggt
     1441 ttcccccaca aaccacatcg tttcttcggg ttgtggtttt ttaacaaaaa aatgcagttc
     1501 cacatcctcc tgctggcggg tgtaaaccgg cacggagcgg ccttcgttat cgctataccc
     1561 cgttactgtc tgacctgatg gagtggtgag gcgacagggg gtattgacca gaatattgcc
     1621 gtgtttgtcc agcaacctat agatagccga gtagatatgc gaagaactca tacgggggaa
     1681 tgccatcggc atactctgcg cagccgccgt catcgtccgc ttcacccgac gcatacaggt
     1741 tgccatcgtg ccatattcaa ccttcccggc ttccagtttc aggtaactgc caccgccaat
     1801 cagcgtaatg cgtttcttgc cggtaaacga gatatcactt tctgacgaaa tggtcagctt
     1861 cttctcagca aacaaccgca tgctgccgtt ttgcgcctgt acctctactg gcccctcccc
     1921 ggatttgacg ctcagttgtc cggtcctggc aaaaagcccc agcctttctc cggccagcgc
     1981 ggtcaggttg cccattgttc cggtactgat atcacctccg gcgttgacag ccacatttcg
     2041 tgctgcactc atctgcatat cttcaccgct ggtcagggca atgccttccg gagccgagaa
     2101 aagcacggct tcattgagcg gtttcagccg ctgcgcaaac atctgtatct gactgtcaat
     2161 atcagccttc agcgcctgtg cccgttctgc ggcattttcc agttgctgca gttgctgatt
     2221 aagcctgtcg atttccttca gcgcggcatc catgtccagt gcctcgccct gtgctttcgc
     2281 ctgcgcatcc gcagcaacaa aaagtccctt ccccgctctc agggttcccc actcatcggt
     2341 acgcagctct gtgcctttac cccgcagttg cccctggctg tccaccagat gtccgctgtt
     2401 aagctgggtc ttaccgtact ccgtggcgag tttgatatgc tcctcgccgc gccggtcctc
     2461 catccgcagt tttgagttag cgaa
//
LOCUS       DAZGTJ010000230         2471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000230 DAZGTJ010000000
VERSION     DAZGTJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.232"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(157..2424)
                     /gene="adiA"
                     /locus_tag="ACHK8E_005183"
     CDS             complement(157..2424)
                     /gene="adiA"
                     /locus_tag="ACHK8E_005183"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP0347283.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          566 a          611 c          677 g          617 t
ORIGIN      
        1 taaataacag gcaaaaaaaa gcgcggggtg ggaccgcgca aaaagcatga cattttttgt
       61 ttgcagcaat ttttatgaaa acgttgccat acttaaaagg cctcaagcca ggcggcgtaa
      121 tgttatttaa caattacgcc ttcagcggaa tagtggttac gctttcacac acataacgtg
      181 gtaaataccg tcaataattt cagtcccttc agtttcgtgt tcaaatccag ggaaatgatg
      241 gtcccaggat tgcagcgagc gtaaataact tacttgcgga ctgtttttat cgccgaagtt
      301 ttcaccagac agcagcatcg ggattcctgg cggatacggg ataactgagt ttgccgcgat
      361 gcgtcctggt aggttttcaa tggataccag ttcgacattg ttgtcgacaa tcgcgttgta
      421 cgcttctcgc ggggtgattt ccgccaccgg caggccggaa taggcttcgt tcaaccgtgc
      481 gccagggttg ttttctttca gccaggcaaa catggtgtca cccagatcgt gaatccccat
      541 gttcgcgtaa gtgtcaggat attgttcaac aagttccggc atcacctgcg ccagcggggt
      601 gttggcgtca tagtggcgtt tgaaggagca aagggtgtta accagagtcc cccatttccc
      661 acgggtcacg cccatagaga acaggaacat aatctggaag tcagtggtac gggtaggcac
      721 aatgccgtgg cgaccaagcc aggctgtgac cagcgccgcc ggaacaccgg tttcttccag
      781 ttcaccatct tcacccattc ccggagcaag gatgctgact ttaatcgggt cgagcatact
      841 ccagttatcc ggaatatctt tgaagccgtg ccagctttcg cccggatgca tgacccagca
      901 gtcctgaacg gtggtcagca gtttggttgg tgcgtcagca aagtcatagg ttttgccggt
      961 ttgtgggtcg gtgacgactt ctttgttcca cggtttgaag aaccagctac cgtcagcggt
     1021 gaactcttta tacagccgcg ccatcgcctg acggaaatca accgcttcgt cgatcacttc
     1081 ctgcgtcagt gacaggccgc tgttgccgtc catcatcgac accgccacgt cgttggatgc
     1141 gcagatggca tacagcgggg aggtggtggc atgcatcatg taggcctggt tgaagcggga
     1201 gaagttaatc gccccacgac cttcacgtac atgaatataa gaggcctgtg acagcgcatt
     1261 cagcagtttg tgggtggagt gggtggcgaa aacggtaggg ccgttgtgat cgccaggttc
     1321 gccgcgcatg gcatagtgat cggcatagat tgggttgaaa cgtgcatagc cgtaccaggc
     1381 ttcgtcaaag tgcagacgat cggaggtttt ttccagcaga tcctgcgctt ctttagcgtt
     1441 ataacacacg ccgtcatagg tacagttggt caccacgcag taagacggtt tttgcccggc
     1501 tttgtctttg gtcagcgggc tttcactgat tttcttctgc aaggtttcag gttgcatttc
     1561 ctgcggatag attggcccga taatgccgta gcggttgcgg cttggcacca tgtagaccgg
     1621 tttcgcgcct gtcagcatca agccttgttc gatggattta tggcagttac ggtcaacgac
     1681 cacgacatcg ttatcggtca tgcaagcctg catgatggtg cggttagagc cggaagtacc
     1741 gacgactacc gaccaggagc gatcggcacc aaatacgcgt gcagcatatt tttcgctttc
     1801 gccaaatgcg ccagtatggt caagcaaaga accgagggaa gttcgttcga tgcccatgtc
     1861 ggtgcggaac agattttcac catagtagtc atggtagaaa cgtccggcgg gtgttttggt
     1921 aaaaccaacg ccgccctggt ggcctggcgc tgcccaggaa tattcatgga tgtcgctata
     1981 tttcatcagc gcgctgaaca gtggcggcaa cagttgctgg cggtagcggg tcatcgcggc
     2041 aacggcgcgt ccggcgataa agtcggcggt atcttccaga atccaggcga attcatcgac
     2101 aagctccagc agatcccggt ccattgcggc gagggctttt tcccgatcgc ccaacaggaa
     2161 gaccggcacg ttttgttgac gctcatgaag cttaccgatc aattgtctga cgttttgatg
     2221 ttcgtccgga tgttccattt gatagctgaa catcaggcag tcaatggctt cgtttgaaga
     2281 gagaatggca aaaccatcat caaaggaggt ggatttaatc acggtaacat tttgctggct
     2341 taaagcatct gccagacgct caacggcgtt accgacccag gtgtcttgat ggagaaactc
     2401 gctttcaaca attaatactt tcatcattgc ttacccggtt atgaaggaat cttcgttgcg
     2461 ggcaagtatc t
//
LOCUS       DAZGTJ010000231         2405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000231 DAZGTJ010000000
VERSION     DAZGTJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.233"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            197..511
                     /locus_tag="ACHK8E_005184"
     CDS             197..511
                     /locus_tag="ACHK8E_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0347284.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            524..886
                     /locus_tag="ACHK8E_005185"
     CDS             524..886
                     /locus_tag="ACHK8E_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP0347285.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            883..1545
                     /locus_tag="ACHK8E_005186"
     CDS             883..1545
                     /locus_tag="ACHK8E_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347286.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPASGKGD
                     NQ"
     gene            1545..>2405
                     /locus_tag="ACHK8E_005187"
     CDS             1545..>2405
                     /locus_tag="ACHK8E_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347287.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEP"
BASE COUNT          480 a          589 c          775 g          561 t
ORIGIN      
        1 agtctgacgg atgcctgggc aggctggcgt agcggtgatg tccgggatgg tgtgttcggc
       61 tggtttatgt tccgactcgt cctgctgctg atctgcagta tctggatgtt tgcgggttga
      121 tttcagtgtc ttgtttaagt ttcaggagga aataatgcat aaaaaaatgc gcatctgtcg
      181 ccaggcctgg tatgccatgg cggtgtttct gtgtaccggg atcacacctg ctctggctga
      241 tttgcctcag atagagccgc caacatccgg tggaggtggt ggacttttcg gacagatcaa
      301 aggctatctc caggatggtg ttgtgctgtt tggtcttgtg ctcagtgcag ttgcttttgt
      361 gaaggtggca gaagcggcgc tttccacctt ctcagaggtt cgtgacggga aggccggatg
      421 ggggcagttt ggtgccatcg tggtggttgg tgttgttctg ctggtcgcca ttatctggct
      481 gctgggcaag tcggcctcca tcattctgta aggagacatc cccatgcaga ccattcgttt
      541 tctgcccgat cgtctgaacg tggagcctgc ggtttttcgt ggttttacca caccggaact
      601 ggggctggcg gccctgacag gcgctgtggc aggggtgatc tggccgttac cgctgattcc
      661 tctacttggc tgggtgatta tcccgacagg gatgttgctg actcccctgc tgctggtctg
      721 gttcggtggc gtctggatca gccggctgaa gcggggaaaa cctgacaact ggctctggca
      781 gcatctggcg aagaaaagca gtgcatgggg tggtggccgg aaaacattta tccgggaggc
      841 gcatggctgg tccctgcgcc gtacccgtag cagggggaaa aaatgagtcg ttttcgtcat
      901 gctctgcggg atcgcgacca gcatatcctg acactgcgta ttttcagcgg tgtgctggcc
      961 ggaattctga tgctggtctg tatcgggtgg atgacggcac cggacagact gacaattcac
     1021 aatccgccgg atctgcgttc cggcagtacc cgtccctggt gggaagtccc gccgtcaacg
     1081 gtttattcct ttgcctttta tatctttcag caactgaatg cctggccgaa gaacggagag
     1141 gtggactacc cggcgaaaat tcatgcgctc tccgcctacc tgacgccatc ctgtcgggag
     1201 attctggaga gtgatgcccg ccgtcgcagt gatgcggggg agctgctgga gcgtgtgcgc
     1261 gtggtgtatg aaattccgga acatggctac gggggcaggt acaacaaaaa tccggtgacg
     1321 gtggaagggc gggataactg gcaggtacag ctggatctgg tggcagatga gtattttcat
     1381 gctgagccgg taaaacgggc acttgtccgt tatccgataa aggtggtgcg catggagggg
     1441 gatcctgaac gtaatccgtt cgggctggcg ctggactgct acagcagtac accacagcgg
     1501 ctggaagcag cctccccggc atccgggaag ggggataatc agtgatgaac ggagctgaac
     1561 gttgtttata ccgtagtatt gtgatggtcc tgtcactgct ggttttacca ctgagcgcac
     1621 gggctgatga gctgatgaaa tgggagcgca ttcccttacc cgttaccctg atggcaggac
     1681 aggagcgggt tatttttgca gataaaaatg tgcgggtggg cttcccgcca gcactgaacg
     1741 gaaagttgag ggtacagagt accggtgggg cagtctatct gaaagcagag agtgtttttc
     1801 cgcagacgcg tctgcaactg caggatgtgg agagcggaga aattattctg ctggatgtga
     1861 cagcagcaga aaaagggccc acagagcccg tacggattgt gtatgaggga agcgtcagta
     1921 cggtcagtca ggctggcaca gcggcgtctg cacgctctgc agaacacaaa aaggtggtac
     1981 ccggcgggga tgacggctcg caggccctcc gtcagccggt gaagtatgca gagcctttac
     2041 ccgttctgtt gacccgctat gcggcacagt cgctgtacgc accgttacgg acgctggaag
     2101 cggtaccggg tatccggccg gttaatacac atctgcccgg gaaaatcacc acactctatc
     2161 cgtcagagcc actgacagtc acgccactgg cagcctgggc tgcgggtggg cgtgcagttg
     2221 ttgcactgaa actgacgagt acggtaagcc gcaaaattat ccttgatccg cgggcacttc
     2281 agggaaaatt cattgccgcg actttccagc accgctggct gggtgcagca ggtacccctg
     2341 aggataccac cacgctctat gtggtgacgg cagaacggcc ggaacaggcg tttatcgcgg
     2401 agcct
//
LOCUS       DAZGTJ010000232         2398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000232 DAZGTJ010000000
VERSION     DAZGTJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.234"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1461)
                     /locus_tag="ACHK8E_005188"
     CDS             complement(<1..1461)
                     /locus_tag="ACHK8E_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFP0347288.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEISTFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVP"
     gene            complement(1464..2168)
                     /locus_tag="ACHK8E_005189"
     CDS             complement(1464..2168)
                     /locus_tag="ACHK8E_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347289.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            2214..>2398
                     /locus_tag="ACHK8E_005190"
     CDS             2214..>2398
                     /locus_tag="ACHK8E_005190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347290.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGSTVH
                     FSTSAATGSTVRFLTSAA"
BASE COUNT          580 a          705 c          504 g          609 t
ORIGIN      
        1 aggtacggtt ttcagctcct tctgttcagc accatccaga gcacagagtt ctccctttat
       61 caaagggggc aatatgtttc cactttcatc actgtcaggc ttttcttcat ctgcatgatt
      121 ttccggtgga aataactgtt tccacccgat aatcacatta actcttccgc gggaacggaa
      181 taactgcccc ccaatcacaa atgttgcctc agtaacatca gcttcctgct ccggcagaaa
      241 ctgtgccaga tagtgctgac gaatcagttg atataccttc agttcatcac cgttcagtga
      301 gctgaggtca aaaacctgcc gggtcggaat aatggcatgg tgagccgtaa tttttttatc
      361 attccagata cgggataccc gtcctgattc cagttcagcc agttgtggaa gcacggacgg
      421 atctgaacgc ccgacagcct caagcacttc agaactctcc tcatgcatcg aggcaggcag
      481 ataaccacag tctgtcctgg gataagttgt tgctttgtga gtctcataaa gggactgtgc
      541 gatatccaga acggtctgag cccccatgcc aaacttccgg gaacaggctt cctgcaacgt
      601 tcccagcgaa aaacacaaag gtggcggtgt tttctccctc ttctgtacaa catcctgtac
      661 aacagcagag gactgctgac ggcacagacc gagaacagca ccggccgcct gcacattcac
      721 acaacgtccc tcatcatcgc agtactgctc agctgccacc cagttactgc ggaaacggag
      781 cccctccttt tccagccaga caatcacctg ccaccaggaa accggcacaa atgtggagat
      841 ctcctgatcc cgtctgacca ccattgccag cgtgggtgtc tgcacccggc ccactgacaa
      901 aaccccagaa attccacgtt cccgtgcctt cagggtatac aaccgggtca gattcatccc
      961 tgtcagccag tcagcccggg cacgcccctt accggcttca ctgagaagcg cagtctgttc
     1021 gcccgggaga atattattca gcgccttcct gatactggcc tcatccagtg cagataacca
     1081 cagacgcctg acacgccctc tgtactgaca gtactccagc aactctctgg ctatcacttc
     1141 tccttcacga tcggcatctg tggcaataac cacttcagaa gcgcgtttta acagcccccg
     1201 gatcacaata agttgttttt tcaccttcgg cttcaccacc atctgccatt gttccggtat
     1261 cagcggcaac acatcagaac gccatgggga accgaactgt tcaccgtaag cttccggctc
     1321 agccagttcc agcaggtgtc ctatcgccca ggtgacagca atattgtcat ctccgatgta
     1381 tccgtcgccc cgcttactga gcgccagttc acgggcgata tctttcgcct gagaaggttt
     1441 ttcacacaga aagagttgca tactcacccc gctttaaccg gtggtgaata aacagaacgt
     1501 ttcttaccgc tcatcacgtc aggatccggc tcccccatgc ggttaaccgc ctcctttcca
     1561 cgggctgtgc cggacaaaat atcagcacgt gtaacactga ctgatttata attttgaata
     1621 atcccgtaaa tcctgcgtac cgcataccct ccctgattca gcaattcatc ccgttttgtg
     1681 cgggaaataa gtccgtaatg aaatacctga aataccttca tgaccagtcg atcatatccg
     1741 atcagaagcc atacacatcg ataaccgacc ggagaactac tgtacacgcc aatatttacc
     1801 ggcgaagagg atttaatatc agaaagggag atctgtcccg gcaggttttt aaggatcccg
     1861 tcaagccacg caagatgctt ttcaattgtt tcagttgcct tcgtcagggc attttccagc
     1921 ctgactaaca tcatatctgc ataaggatta tccctggctg aatctctggt tgcctgaccc
     1981 gcaaacgcaa tagccctggg catactgatg atgtcaggcc gcttttcctg tttttcttcc
     2041 ttctgattat tcctgcgtcc ctcccagaga cggatcgcat atcgggtatg aagggcaata
     2101 gtcagcgatg actgcagagc accaacttgt tctgattttc tgacttctcc ggttttctcc
     2161 tggggcattg tgtttactcc gaatgatttc atgaccagaa gatagtccgc aggttgcgta
     2221 ttaccgacaa caaaaaaaca aatcaggcag aatatacatt taacgccgct tattttttaa
     2281 tcgctgttgc cactggcaac accgctcact ttttaacctc tgttgccact ggcagcactg
     2341 ttcacttttc aacctctgct gccactggca gcactgttcg ctttttaacc tctgctgc
//
LOCUS       DAZGTJ010000233         2389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000233 DAZGTJ010000000
VERSION     DAZGTJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.235"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1434..>2389
                     /gene="subA"
                     /locus_tag="ACHK8E_005191"
     CDS             1434..>2389
                     /gene="subA"
                     /locus_tag="ACHK8E_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP0347291.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          701 a          500 c          527 g          661 t
ORIGIN      
        1 gagtaaaatt gaaatgcaga gacaccggaa ttgatccaag ctaccgccat tatcttccgc
       61 gctgacagaa gataataaaa atgtgagata cttcaatttt attgaaaacc tgaaaaaccc
      121 ttacaatcct tgcagtttat gaagaaaatt ttcacaaagt aaatttaaaa ataaacaaat
      181 taaatgtaat ataatcgggg gaggattaac catcgatgat tgccggaaca aaaaacaata
      241 taaaccaaca agatagaaaa tcatcacaac aaagggaaag gtctgtggat aagaggggga
      301 ggaactttct gcttcatgtc acataattga tatactttat cccgcaacct ccagttgcat
      361 tatatataat tcaacctcag gctcccatca caggccaaaa ataatatatc gcaagccgca
      421 tcaaatccgg atttttatat aataaataat acctgttttt tccatcacca gtctgttaat
      481 caacttaata tatttcaggg ttattttaca ttagcccggc aaaagttatc aggcatgcag
      541 gatacattat cagggagagc ggtagtgaac gccggtacct gcttccccaa agttatttaa
      601 ttaacccgta tcaacagggt aacagcatgg caaaatccag caattacgca cctgttaagg
      661 tcattattta cgttgatatc ggtaaagatc cctgccacac tgttatcatt aatcgttctg
      721 ataattagtt cgacaaaata ttagcaaacg tcgaaaacaa gctcaggaaa cagatatccg
      781 gcctcaggaa atatgggcat atactgctga ttgctgacca gcctgctacc atcgttgtat
      841 tacccgtcca cattgcacgc tcagaaggtg tccttgtcag atacctgtaa gtggactgac
      901 tgccccgtat tgatatctat tgaccagtca gttcgacttt cccggggagc agctcgcatc
      961 cccggtttaa cggtcattca tgaatatact cagtgacctg atatccgggg cttaccaatg
     1021 aaccacacca ccattcactg attgccccgg taaaacggcg ctgtgatatc ctgttcgcca
     1081 tgatgcctga cggaactttt tattgtcata aacatgccag gcaacttact tgcctcctct
     1141 tattttttct ggttaagttt tatccatcat ggctgtattt acttctgctc tgaaggagga
     1201 ggcagagcat cttatcgctg acctgttgca gaaatccaga tgcatattac cgtgaaggtg
     1261 agcgttaatt cattggatac ataaatataa aaattaatgt ttttgagacg ggcttcactc
     1321 gctttattgg ttgtgattgt ttatcttttg tcgaaaacag gatatttcag atgtgatgtt
     1381 cctgtaagat attgacctgc aatactgttt atttgtactg actggaggat attgtgctta
     1441 agattttatg gccgcatatt ctgttcctgc tttttatatc tgtgtctgtc agggcagaaa
     1501 aaccctggta ttttgatgca attggcctga cagaaacaac catgtcgctc acagacaaga
     1561 atacccctgt tgtcgtatct gttgttgatt ccggagtagc atttgtagga ggcctcagcg
     1621 acagcgaatt tgccaaattc agttttactc aggatggttc gccattcccg gtaaaagagt
     1681 ctgaagcttt atatattcat ggtactgcca tggcttcact cattgcctca cgtcatgggg
     1741 tttatggtgt ttatcctcat gcactgatat ccagcagaag ggttattcct gacggtgtac
     1801 aggactcatg gattagagca actgaaagca ttatgtcaaa tgtttttctt gcaccaggag
     1861 aagagaaaat cattaatata tcgggaggcc agaaggggat atcttccgca tcggtctgga
     1921 ccgaactgct ttcccgtatg ggccggaata atgagcgatt aattgttgcg gcagtgggta
     1981 atgatggcgc agatatacga aaactgagtg ctcagcagag aatatggcct gcggcttacc
     2041 atccggtcag cagtatgaat aaaaagcggg atcctgtgat aagagtggca gctctggcac
     2101 agtaccggaa aggagaaaca cctgtattgc atggtggagg gatcaccgga agtcggttcg
     2161 gaaacggctg ggttgatatt gctgcaccgg gacagaatat tacattcctc agaccagatg
     2221 gaaaaacggg gacaggtagt ggaacctcgg aagcaacagc tatagtttcc ggcgttctgg
     2281 cagcgatggt ctcatgtaat ccccgtgcaa cagcgacaga actgaagcgt acactgctgg
     2341 agtctgcaga caaatacccc tcacttgcag ataaggtgac ggaagggag
//
LOCUS       DAZGTJ010000234         2288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000234 DAZGTJ010000000
VERSION     DAZGTJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.236"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            111..320
                     /locus_tag="ACHK8E_005192"
     CDS             111..320
                     /locus_tag="ACHK8E_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0347292.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(340..573)
                     /locus_tag="ACHK8E_005193"
     CDS             complement(340..573)
                     /locus_tag="ACHK8E_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347293.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(662..1285)
                     /locus_tag="ACHK8E_005194"
     CDS             complement(662..1285)
                     /locus_tag="ACHK8E_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0347294.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1374..1883)
                     /locus_tag="ACHK8E_005195"
     CDS             complement(1374..1883)
                     /locus_tag="ACHK8E_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347295.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          637 a          601 c          488 g          562 t
ORIGIN      
        1 gagaaaatat ttttaaaaac agaaaattaa caacgcacat caggaaacgt cgtctcctga
       61 ttcagtgata ccacatgaaa atttagtgaa cggttaccgt tctgaagtct atgtcagaag
      121 atattccatt accaaaaatc aatcagcgat acagagacga tcatggcgcg cttgtcaccg
      181 tgaccagtgt tgaggaaacc cgggtggtgt ttatgcggga cggctatccg catccctgca
      241 tgcggccgat gtacaacttc ctgggaaaat tcaaacccga accccggaaa gaaccccctg
      301 ccggcaatca cactgcctga tggcagcata atccacttat taattctcat ccccggagac
      361 aagcccagaa aaccactccg ggtgttcact gtatgccgtg ttcagcgcat cccaagaaac
      421 gttgatataa aagctgtcct gcaatttgcg ggtaacaatt tctgccaccc ggttacggtt
      481 atccgcctgt ccggcataac ctctctctgc cagccactca ttcagttcat gttccggaga
      541 cagattgata atatcgtaat cggaccttgc cattcccgtt tccttagcac atgttttcag
      601 gctcggggat aaagaatgtc gtactgctgc tgccagagca atattccccc tgatggtcga
      661 atcagagatg gtaggccggt actttgcggg ttgttcccgt cagattatca tgcacctccg
      721 cggtcgttgt caccaccggc attccgctct ttctcagcct caggacatca gccgcaatac
      781 gctgactacc gaaccagaac aggccgtcca gtgcagtcac cagcaacccc gcctccagcg
      841 catgcttcag ccgttcacgg ggcgctttca cttcccgggc aatctgctgg tatggcgatg
      901 atgtgttttc attcccaatc acccggcgaa tacgatgaga cagatgatac cggtatgtat
      961 ccggtacacc ggaaaggctg gccttcaggc tgtacacgca gccaaatcgt ttatcattga
     1021 acaccacatt tttctggctg atgccccatt cttcacgcag cgcggcaatc agttgtggtg
     1081 tccgggtaag caacaagcga aaaggcagtt caaaactggt gacgtaatcc acattcagca
     1141 gtgcaatacg cagccgttct tccggcccgg cctctgtctg ccggcactcc tccagaacat
     1201 cctgccactg ctggtgaagg cgtggaagtt cattcagttc agtaaaactg tatttatccg
     1261 caagatagtc aattcgtgta cgcatactga agagtattcc gtataaagat tcagctggca
     1321 aaactttatc agtctgtaaa aactaacgga agagtcgata tttctcccgt ccatcaccgg
     1381 atgattgttg caataccccg tggcatcaga gactgaacag ccgtttttaa cgccacgtat
     1441 tgccctgatg aaccaggccg ggcaacccga aaacatcttt ccacccggca ttgtccgcca
     1501 gcacttatca ccggccaggt ctgttgcagt aaatccacca gttgcgcaca ttcttcatca
     1561 gccatgacgc cgactcgcgg atggcaaata ctcgtctggc tgagcagcaa cagacatcgt
     1621 atctgtgctc cttcatgttg ttcccgcaac cagcgtaata ccaccggcgc tgatgggctg
     1681 accgtgtgat tatgctccgt aatacgttcg tgtacccgtc ggtgaaagga actacaaaat
     1741 gtctgaatct gttgaccatt gattccttct gccgaataga gcgtgaagtg aaatgccaac
     1801 agatttggcc agtggtccac cgcctgctga acaaaacgcc ggatttcccc cggctctgaa
     1861 attaaggctt cggttatttg cacgaaatgt tccttttcaa cccctgttga attttgtcaa
     1921 gccggtgcag gcacagtaaa gcgcgcatca acacctgaaa ggtacgatac aaacacaagt
     1981 tacaaacaat aagaacggaa ggacaattaa tcagtgagga ataataacta aattaaccaa
     2041 aagaatgtta ctgacaatac taccaatact taaatagcaa tatattaatt aatgtaagaa
     2101 cagatgcccg gtaagacata tacacaatac atccacaaac acactctttt cattaaatca
     2161 atcacctgat caaaccatga aagtgtgatg taagtcaaaa ttactgacac tatcaattga
     2221 taatgattat ctctatcatt atcgtgaaat tcgagaatga aaggagaagt agtcattata
     2281 agcctttt
//
LOCUS       DAZGTJ010000235         2263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000235 DAZGTJ010000000
VERSION     DAZGTJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.237"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2047
                     /locus_tag="ACHK8E_005196"
     CDS             <1..2047
                     /locus_tag="ACHK8E_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0347296.1"
                     /translation="KSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
     gene            2080..>2263
                     /locus_tag="ACHK8E_005197"
     CDS             2080..>2263
                     /locus_tag="ACHK8E_005197"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0347297.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNP"
BASE COUNT          710 a          428 c          544 g          581 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacagctg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggtag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctggcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaaag attgttcagt attcagacac ctaaaaagaa atttaatgcc
     2161 aataaattaa catttaatgg caactatcct tatgttgcca gaggaagtaa caataacggc
     2221 ataaaaggat atatagatga agatgaatta tatctcaacc cgg
//
LOCUS       DAZGTJ010000236         2255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000236 DAZGTJ010000000
VERSION     DAZGTJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.238"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(332..1157)
                     /locus_tag="ACHK8E_005198"
                     /pseudo
     CDS             complement(332..1157)
                     /locus_tag="ACHK8E_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1308..2081)
                     /locus_tag="ACHK8E_005199"
     CDS             complement(1308..2081)
                     /locus_tag="ACHK8E_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0347298.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            complement(2106..2255)
                     /locus_tag="ACHK8E_005200"
     CDS             complement(2106..2255)
                     /locus_tag="ACHK8E_005200"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0347299.1"
                     /translation="MGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAG
                     VGWQW"
BASE COUNT          551 a          623 c          460 g          621 t
ORIGIN      
        1 ataaagagtg ggatggactt cttacttcat atcacataat tgatattttt tatcctgcaa
       61 actccaacgg cattatgtat gacttagcct caggcaatta tcacaggcaa aaaaacaata
      121 tcttgtaagc ttcatagaat ttggaacttt atataataaa taatgctgag ttttttatca
      181 ccaatacgtt aacaaaatta atgtattcca gagatttcac attagtctcc aatttttcag
      241 aagaatctat ttacccggca ggaaaagtca tctggtgttt cgccggagtg aggggtacag
      301 gcaatagcgg acataaccat ttgttctttt tctaagcagc catctgataa tattttatcc
      361 tgaaggctgt cggggagata ttccccagac gagagtgacg acgctgacga ttgtagaaaa
      421 tctcaatgta ttcccgtatt actgagatgg cctcatcccg gcttttaaaa cgatagtggc
      481 tcagactttc atttttcagc gttccccaga agctttccat cggagcattg tcgtaacagt
      541 taactttacg cgacattgat attttcagac caaactgctc ctgtatggcc ctgtaatcgt
      601 atgcgcagta ctgtgaacca cgatcggagt ggtggattag cccggtaggt gagcgctggt
      661 tcctgagcgc cataaacagg gctttacctg tcagctcttt tgtcatgcgc tctcccatgg
      721 cgtagccgac aatttcgcac gtataaacat ctttgacgcc agcgatatac agccatcctt
      781 ccggggggca acatacgtta tatctgcaac caagacctga ttaggtgctg taggcgtaaa
      841 gttctgattc aggagattcg gcgcaaccgg cagattgtga ccagagttcg tgttcgccct
      901 gaacttgcgt ttcagcttac agtgcagcct cagctcctta cgaagacgtg ccagtctgtc
      961 acgaccgacg ataattccgt tatctgccag ctccgtctgg agcagctagg ttccatatgt
     1021 ttcgcgtgtg cggatatgtg ccaccttaat ctccagtttt agccgctcat cactttgttt
     1081 tctgtctgag ggtcatgctg tactcagtcg taataaccga tcctgtatac accaagtacc
     1141 cgacacatcg cttcaataca taatcgcatc tgaatcgttc accgattcgc ggcatttttc
     1201 aaggtagttg tcgccattgt tcgactaact gatcgacatt acagcctaga gcctagctgg
     1261 gggctgtgca caaaaaagcc ccggacattc cggggctaca ctgcatatta acgaacgctc
     1321 tgtccggtta tttcaaccct gcggttgggg cgcagacatt ccttcagtgc ttcgccctcc
     1381 acatccgggc agaacaccac tggtgcatca tatccacggc ccgttgcatg tatcctttca
     1441 gccctgaatc cccgggcaat aaaccacccc ctgactgatt ccgcccgttt ctctgacagg
     1501 agccggttat acgcctgccc gccaagatgg tccgtaaatc cttctatctt caccccgaca
     1561 acatgacccg cctgaaactt ctctgccaca cgggaaagtg catcacgccc cctctctgtc
     1621 agagtccatg aatcaaaatc aaataatata tcaccgggga ctgactccct gtattctgct
     1681 gtctctgcct ccggcagaca gttttccggc ttccagtaga aagagcgggc atccatgttc
     1741 cggtcaaaca gtatcttgta ctggcaggta taatccccct ccggtccggt aaagttgaac
     1801 aggtaatccc attcccttac accagacatc ccttcagcaa agtgtggtct ccccgccagc
     1861 tgataaatct ggtccttatt catacccgcc tttatctgac gtaacttttc tggcagtggc
     1921 cacgaacctt tctgtgttcc gtcatgatta aacatcacct tctccggaga aggccatacc
     1981 acttcttccg tcgttccttc atccgtgaca tgactcagat ttccacatcc tgcaagcatc
     2041 agggacagca cacccgtcat catcattcga ctaaataaca ttacatttct ccttgctttt
     2101 ccctgttacc actgccagcc cacaccggca ccgacggaga aattaccccg ggtgttctga
     2161 cctgcattca gtttcattat catcttaccg ttatcggaga ttcgggacac acctattgca
     2221 atggcgcttt caccaccata tccaccgaca cccag
//
LOCUS       DAZGTJ010000237         2225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000237 DAZGTJ010000000
VERSION     DAZGTJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.239"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(110..1108)
                     /locus_tag="ACHK8E_005201"
     CDS             complement(110..1108)
                     /locus_tag="ACHK8E_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP0347300.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPAARWLFLTLTVRNCSIDALGETLTSMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSVHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
BASE COUNT          546 a          647 c          543 g          489 t
ORIGIN      
        1 aaaaaaaacg ggcaaaacct tgcccgtttc tgaaaatcac gttagaacgc ttctgagcgc
       61 gtttctgaat attctcccag tgcatgaata gttcgcgcct gaaacgcgct caatcctcgc
      121 cacttagctg acgtttatct ttcgcccgat cgcgctggta ttttttgaca accggcaccc
      181 acgaaaacgc cagacgggaa ccgtcatctg taccctctgc catgccatcc gccatgatta
      241 aatcctcgtt cgtttcccgc tccagttgca gaacgttctt aagcgctccc cccgtagcaa
      301 caaaacgacg cttgtgtacc tgccgtgtta attcgaggaa ccattcagga tcagccagca
      361 tatcagcagg ctttgtggaa tatttcagag tttcaaccac cgcgccgcgc agctgttccg
      421 caacaacaca aggtaaagat tcatcgtctt ttggtttccg tgacttcacc actctgatat
      481 ccacattcgg gtcatacgcc acccgcagac aatcacgcca cagttccacc caacgggcat
      541 gtttcacata ctcacggccg gacaacatcg aagggggaac catcagcaga gtgtgaaaat
      601 ggggatgtac gctaccatcc ttgccgcgcg ttacttccgt ggtacgaacc cagcccaaca
      661 catggcgaaa ttccttacgc tctttcaacc gcttaaaccc cgcattcatc gaggtaagcg
      721 tctcaccaag cgcgtcaatg gaacagttgc gcactgtcag tgtgagaaac aaccagcgcg
      781 cggcgggatg ttccgtcaca atctttggca gggactgata gaaacgggct tgccacatca
      841 tggaacgcct ccactgacag atcggacagt gccggacgcg acagaaccgt gcatcacgca
      901 accgcaggcg cgtttctccg gtctctgtgt ttgttgacca cccaaaccgc aaaataccac
      961 tacaagcccc gacgcgttcg ccgtagtgct caaatccggc agcacgcaga taaataccgc
     1021 ccacatcgtc ggaaaagctt cggtgttcat cccacggttt atctgctgga gagtaatccg
     1081 tcagattctt aagttcagga tttttcatgt ctgccgtccc cttgcgagcg acagacgaaa
     1141 ttgcgtgtta tcattcctgt atcacctgta ccaattgtcg tcaaatcagt tcatgttcta
     1201 ggtggtcgcg cccctggagt ggtggaagac tcgcaggggc taatttataa ccactcccga
     1261 caaaatactc tcaaacctat ttacacgcaa atccgcgctc aggccagata tggcaaggga
     1321 tagaggcgat ccaaaaaatc gcatttatcc cttaagtata aatacaagga aacgcgcgcg
     1381 aagatagcag caaaatcgcc tgttttttgt tcgattcccc gagggcaaaa acaacaaccg
     1441 cgaaagcgtt ttcccgacaa aacacacaca ggcttttcca ccgattttgt gaacagcaaa
     1501 aaccgtcttg caggtggttg gtctcactcc gtgacaccaa ccaccatccc cagtgttttt
     1561 cccgtgccgt cgtcaatcac aaaatcggaa ccccggaggg gcccccgcga ttttctgttt
     1621 gacgagttcg tgaacggtga gcgggacgcg gaattttttc gcgtgccgtg aggcgttggc
     1681 ggggtgtcgg ggtgcagccc tgaccaggtg gggaatgtct gagtgcgcgt gcgcggtccg
     1741 acattcccac atcctgtccc gaaaattccc gccgtttctc ggttttttga gcgtcgcaga
     1801 ccgcacgaca aacgaaacga agtgtagttt gtatgagtgc gcacttcgtg agattccccg
     1861 actgccagtt tgtcgatcgc gcggaacagc tgtggcacga aaacggcaga atgatttgct
     1921 gtccggttgc cagaactgcg cgcttacgtg gagggcggcg cggcgcaaaa actgccgaca
     1981 ttgcgccagt gttccggtga cacaaacccg cagaaatcgg gacaggatgt gtaatcgtat
     2041 aaccgcgcac gcacgccgat acgattacct cctggtcagg gctgcacccc gacaccctcg
     2101 acaacctgcc cgggacacgt taaaacgaca tctgcgcgtt tctggtgagc gaaaagctgt
     2161 cagccgtgga ttgccgtagc agagacccgg aaacgcgctt aaaacgcttc tgagcgcaga
     2221 gaaac
//
LOCUS       DAZGTJ010000238         2212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000238 DAZGTJ010000000
VERSION     DAZGTJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.240"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..701)
                     /gene="tssG"
                     /locus_tag="ACHK8E_005202"
     CDS             complement(<1..701)
                     /gene="tssG"
                     /locus_tag="ACHK8E_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0347301.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGKR"
     gene            complement(665..>2212)
                     /gene="tssF"
                     /locus_tag="ACHK8E_005203"
     CDS             complement(665..>2212)
                     /gene="tssF"
                     /locus_tag="ACHK8E_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0347302.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIVLSEAWSSCLPFET
                     ENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HNLHVPG"
BASE COUNT          489 a          620 c          646 g          457 t
ORIGIN      
        1 atgcgttttc cggcacccag cccactgcga ttatcgatat ggaccttaac cggatcgggt
       61 tcggtgataa gtgcgcgggt atccggtgcc agcagactca ccagagcccg gatgccttca
      121 gcattgcggg tgggtaatcg catggcaccc agtaaggcca ggaaacggga aaccggcgtt
      181 gccacctgtt cagcggtgcc gggtataccg agcccgacca gccccagcag acactgcgat
      241 gtggcatcgc gccccccctc ctcaaaagtg gccgggtacg catattttcg ccagatccgg
      301 tagtactgcg tggtgatgcg gtggctgaaa atatccagaa atgaggtgag cgcctcatgt
      361 ccttcacgac gctgcgtaat atcatccagc caggacgtcg gcagaggtga gtccacccca
      421 tacattccga gaaatgtcgt gcggattgtt gggggtaatg tcggacggtc ttcgtctatc
      481 tccaccgctt tcagggtgct gacagggaat cccatcccgg gccagggcct gaatcgcacc
      541 ggatcatcgc ccggatggct ggtcgtgccc agttttggcc cgtccggatt ttcctgctcc
      601 agcagctggc agaaacgata aaaactggcc cgccagatat cgttgttaag ggccagggcc
      661 tgcgtcagcc cggtacgtgc aggttgtgat tctctttcca tctcagtcgt ttccctgtgg
      721 gttgcagtac cagtgtgagc tggttgaaaa ggtggacatc agcgtagagc gcaaagaaac
      781 ggtgcagcat ctcgccgaag agattcacat cgccctcgcc ggcaaaatta tccatattca
      841 gcgtgacctc aatgtccacg ccacggagca taaagccctt ttcaaaacgg cggatcaggg
      901 tgtgcttcac ggcaacgatg gcttcaagcc ggcggcggtt catttcatcg tcggtccagt
      961 catagagcgc cagcgtcccc cgcagcacct ccggattgtc catcatgctg agaaagtttg
     1021 aaccgaggtg actcatcacc cgccagtgaa accggtcatt tgccggggga tacagtggca
     1081 tcgtcggtgc ggtcaggttc aggactctga cggggacttt gccggttttg accaccctgt
     1141 ccaggagcgt actttccagc gccctgcgcg gaagctgccc gttggtcgcg gtgattcgca
     1201 tcgagaggga ctccggttta tcagacaact gctccagttc gaacgaacgt cccccgagga
     1261 ctaaccatgt gtcatacagg ccggagggtc cccgtttcac gcgggtgtgg taataacgtt
     1321 caggagcatc gtggcgcatc ataccgcccc ggtgccggaa gctggtgaac gggacataag
     1381 ggtgtctgcc attcttcacc gagccgtgaa tgttatcgac gctgtaaatt tcggtgtgac
     1441 catcctgaag acgcagcggc ctgagcaggt actcgttatc cagcgggtta aggagcagcg
     1501 ggtcggcttc cagggtaaag agattgataa ccggcgcaca atgcaggcgg aagttttctg
     1561 tctcaaaagg caaacaggat gaccaggctt cactgagcac aatgtcgatt tcaaaccagg
     1621 tactggccgc agtgagcccg atggtgtcga ggccgcgcag ttcaacaaac ataaacttcg
     1681 gacggaagct gaaatactcc agcagcaact ggtaaccact gaaggctgta tcggctttct
     1741 tccacaggca gtcattgtcg tcaaagccca tgggtctgca ccagccgtca aactgatgac
     1801 gtccggtata tgtcccggca tgacgcgcat acatggcatg tacacgccgg gtcattgcca
     1861 gatgcagagc agcgcttacc ggcgcctctg cattaagaaa aatggcgact ttgtcgatcc
     1921 cggctttact ccagtccact ttttcagggc attcgaagcg caggcggatg gcagagcggc
     1981 catcggattc agtatggaga cgggcatctg caaggtgcag cggttgtagc aacacatcgc
     2041 gggtcgtccg gtactgacag gcggttttat gcggcccgac aggacgggac aaaacggaga
     2101 agccttcagc cagtatttcg gcctgacgca gactacgcca gtcaggcgag aactcaacga
     2161 tggccagcga cgggatagtc cgcatatagt gcggccacag cagactcacc ag
//
LOCUS       DAZGTJ010000239         2153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000239 DAZGTJ010000000
VERSION     DAZGTJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.241"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..560)
                     /locus_tag="ACHK8E_005204"
     CDS             complement(72..560)
                     /locus_tag="ACHK8E_005204"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0347303.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(566..847)
                     /locus_tag="ACHK8E_005205"
     CDS             complement(566..847)
                     /locus_tag="ACHK8E_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0347304.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            complement(1265..1840)
                     /gene="dolP"
                     /locus_tag="ACHK8E_005206"
     CDS             complement(1265..1840)
                     /gene="dolP"
                     /locus_tag="ACHK8E_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0347305.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPGRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
BASE COUNT          557 a          524 c          476 g          596 t
ORIGIN      
        1 tgttgcccgt taacatttaa cttcgtgggc tgacgttgcc acaagcaccg tatttccctg
       61 atttattctg tttattttat tgtggataat gatgccaatg gcatgaataa agttcttccg
      121 gactccggga acgccataaa gccataatgt ttgtaaaatg cgactgccgt gtcatctttt
      181 gcatcaacaa taagcgcata tgctgcaatt tcagatgtgt atgcacgata tagcgcatca
      241 gctaaaagtg ctcccccaag ccccgttcct tttgatgaca gacttactgc cagtcgtccc
      301 atacgaatag ccggaatagt gggatatcgc ggaagctttt ttctcaaatg ctcaggtaag
      361 tcagttaata atatactggc cgctgataac gtatagtatc cgatgatatg atgatcgtta
      421 tctacagcga caaaacaggc cgcgatgcgt cgtcgaatat cctgtgtgac atgctctttt
      481 atgtaacggt ccagagcatc agagccacat aaaaatgagg ttctgtcata tgtgctgtta
      541 aatggtgtca gttgaaacat tatatttatt ttgtgtacag catattatta cggcgtttaa
      601 aggcccgttt aagcgcatca ttcggctctg ttggtgacag taatgcctga gcaaaaattt
      661 cctgatctgt ccgggacagt cggattatat cggtttgttc gatagcgcgt tgggccgcat
      721 cctgaacggc agcaatgata aaatcggtca tagtgcgccc ctggatttct gcagcgcgct
      781 tcagcatggc atgtagttct gtgctgatcc ttgcttccag tcgggcagtt tgtgttgcag
      841 atggcatgat ttgtacctcc tgaatacact gtacggcaaa ttgccgtatt gtgcaaatta
      901 cactctgaca ccgggacggc agacagacat tctgcctgaa aagaaaaatc agattcagac
      961 aaaactatcc gggggatgtt aacgccacga tctttgacca ttgcatacag ataaaagctc
     1021 agattattta ccagcagtga catccggagt acaccgggat tttatctgtg agtgaacata
     1081 cccgggcaaa aaaatccccc gaattcaggg gggtgtcaaa gaggaattat gtatattggt
     1141 tattataatg agcacgcctc cggtattccg cgacgaatgt tcacgtggtg gcatgaacgg
     1201 cccggaaacg tgctcttctg atttttaacg taaatttaca ctaaaggcaa ctctgatact
     1261 gccttcaccg gatataggag aaggccgtcg tgacccgctt cactccgctc accctactgg
     1321 caacatctgc tgccgctcgc ccttcctgct ctgtaaccag gcccagtaaa aagacttcgc
     1381 tgttttctgt caccaccttg acactggatg atttgacacg ggcacttgtg agtaactgag
     1441 aacgaatttt tgtcgtaagc caggcatcac ctgccattat gaccggactg acgggtgtcc
     1501 cctgccggat ttcattatag acctcctgaa caccatcgac accgagtgct atctgacggg
     1561 cacgggcaga taattcacgg cccggagact gaccgatcag cagcacgcgt ccctgatatg
     1621 caatctcact gatgcgtgct tcttttctga tttgttcatc ttttgaaagt gcactgctga
     1681 ccctcagctc cagcacttca tcatccacct gcgctccgac actgcgtggg tcggttgctg
     1741 cctttgtccc gacagcagct gtacccacca cagcggcagc cacgcatccc tgtaacaata
     1801 aggctgaagt aaggacaaca gcagaatata ataaacgcat atctcccccg gaattgtttt
     1861 gatttttact tcatattccc ggaatttatc gtacaaaaag tttaccttca gtgaaacaaa
     1921 acaagctaca gactgttatt atcctccggc acagccggaa atagcggatg cgttacgtgg
     1981 catatctgta tctgtttttt gcacgaaaac tttgataccg tcgtaaagat tcttttttta
     2041 actatctggt tcgctgaaca ggatctcctt ttcgttatac acaggctaaa aggcaccgtg
     2101 gagcccccgg tgcctttttc ataaaacaga gaaaacacgt ctttgaatat acc
//
LOCUS       DAZGTJ010000240         2072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000240 DAZGTJ010000000
VERSION     DAZGTJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.242"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            341..472
                     /locus_tag="ACHK8E_005207"
     CDS             341..472
                     /locus_tag="ACHK8E_005207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347306.1"
                     /translation="MDSENKIQVHKSIDYCIGVVGINNDDVKVKISIQDGNDSKNSE"
     gene            679..924
                     /locus_tag="ACHK8E_005208"
     CDS             679..924
                     /locus_tag="ACHK8E_005208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347307.1"
                     /translation="MEEMQDKMAWMQNLITEKLGPSLWHMHPIAFPGALIKKNTGWAH
                     SKFANLLGSVESENDYSAYNVTKTGKSVPHYNINLTK"
     gene            1095..1673
                     /locus_tag="ACHK8E_005209"
     CDS             1095..1673
                     /locus_tag="ACHK8E_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347308.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSISVS
                     KSDNPVCKTTLFNFNNNEYVKLIESLDGCNNQ"
BASE COUNT          732 a          293 c          409 g          638 t
ORIGIN      
        1 acaaaatatg aagcaagtat taaagtaaga gaaaggctaa aattaggaat aaaagaaaga
       61 tatagaagtt aacatgagaa aaactctatc tttgatagtt gcacttttac ctatgtatag
      121 catagacaac agcttcgatt ttttacagtt cattcaataa aaatggaaca caatgtgaaa
      181 cctcttttaa tgataaagtt gttttgaagc ataggtgtga gtatgaatat ccttcctgtt
      241 tgatatctta ttctctcctt gcttactctt ggacagaagt ttgaatattc cattccccat
      301 gggaaatact tgcgaggttg gagcaaaaag aattatctct atggattccg aaaataaaat
      361 tcaggtgcat aagtcaatag attactgtat tggagttgtt gggattaaca atgatgatgt
      421 taaagtaaaa attagcattc aagatggtaa tgactctaaa aattctgagt gaacaacaaa
      481 ttagcaaagt taaaatagtt gcgtttctta tctggtcggt ttgatatttt tgttagtatt
      541 gccatacttg taaagttaat caaatatcat caattgattt agtgtaattt caaaatatta
      601 atatatcaaa atacaatgag ttaaacgact ttataacgaa ttcaaaacta atgaaaaact
      661 gaaaaaagac gtaccggaat ggaagaaatg caggataaga tggcctggat gcagaatctg
      721 atcacggaga aactgggccc ttcgttgtgg catatgcatc cgattgcctt tcctggagca
      781 ttaataaaga aaaatactgg gtgggcgcat agcaagtttg caaacctttt gggaagtgtc
      841 gagtcagaaa atgattattc ggcatataat gttactaaga caggtaaatc tgtcccgcac
      901 tataatatta atctcacaaa atgacattat ctgaggtaat tgatgcccca agagaacgaa
      961 aaatggtaaa aagaaataca ggttagtacc tagggtggca gaagttgagg gggagtcgta
     1021 ctattctggt gatggattga ataaagctca cctattacca gatacgatga ttaaagctct
     1081 tgaggattct aaaaatggga tgcaataaaa aatcaaacaa atttttgctt tttgtgtcat
     1141 gcatgctctt gtcgtttgta acgtactcac aagacagttc taatcctcag ttttctgact
     1201 acctggttcc tgtaagcaac ggaccatttg aaaagaatat tcactttaat aaagagcagg
     1261 aaaattactc gcaacactgg aagaatgcag tccaagagga actaaaaaag cctgttaatt
     1321 ttgcagggca ctttagaatc tatacagcct ttggcggtca tggtaaagaa tgccttcgtg
     1381 ataattgggt atgtggttgg gttattgata aactatcagg ggaagttgta gcaacacttc
     1441 catcagataa taatggaagt tataactatg ctgatgtgtc tgataatggt acccctgttg
     1501 gtcttccatt tgagattgat acttataaaa atagttcaat gattgcaata accggtcaat
     1561 ctatctcagt aagcaagagt gataatcctg tatgtaaaac gactttattt aattttaaca
     1621 ataatgaata tgtgaaatta attgaatcat tggacgggtg taataatcaa taaatctctc
     1681 atgaatattc atcataatga gaaaacgact gtagtaaaat tcggatgcga catgtatcag
     1741 tgaacgttca gggttgagtt aatagattcg actctaaatc cgtgataaat gttaatttaa
     1801 gctaagcaaa tggacttaag aaaaacatgg cttatttgca ttctcacatg taataatttt
     1861 gctgttgtag gtgaaatacc aagggtaatg atttcctcag aaaagaatgt ttgtggtata
     1921 aaaattaatg acgagtttgt aaatatgtag gtggttacaa atgtgaattt tattgcgcac
     1981 tatctgtagt atcgcatcca tatttgtatg aaattggcaa aaaataatag tatttattga
     2041 taacccaatg gtaaatgcat gcgatggaga ac
//
LOCUS       DAZGTJ010000241         2065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000241 DAZGTJ010000000
VERSION     DAZGTJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.243"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            180..647
                     /locus_tag="ACHK8E_005210"
     CDS             180..647
                     /locus_tag="ACHK8E_005210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347309.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
     gene            complement(770..1051)
                     /locus_tag="ACHK8E_005211"
     CDS             complement(770..1051)
                     /locus_tag="ACHK8E_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347310.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
BASE COUNT          560 a          368 c          438 g          699 t
ORIGIN      
        1 tcatggcagc ctcatgtatt tatgtggaga ttatttcatc ggatattatt ttatccagga
       61 atgttgtttt gttaaagtga gtaagtctta tagattatgt ttctttactg aattccgggt
      121 atgtagacaa agctggttct gtatatgtga acttctttgt tgttcctgag ggagataata
      181 tgaaaggcaa gattatacca gaaaaaaata agataaaaaa cttaatcaac caggagcgtg
      241 acgccattgc actctggatt aaggatggaa tgagactgcc agatatttac agattactga
      301 aggaaaaaca cccggaactg acgttttcac agaatggttt tgtattttca ctgagacgtg
      361 atgcaagtga tttacatgaa aatgcgcttt ttaatcgcag tactgttgct gtatttatgc
      421 agaaacaaca ttctgagatg actctcatgg ttaattctgg ttgtttgctg aaaaatgtgc
      481 atgaggcgtt attcagtcat atatcttact ccgtgtttct tcgatatatt gtgaagttat
      541 atccagattt acactatcag gcaaaattaa acaggaaagg ggctgtctgt ggtgaattac
      601 atcatgagga gaatgcttct gtttatcaga atgacgaggt ttgttaaagg tgttttataa
      661 aaatagtggg gtagtgctgg cacagagatg tcttacaggc attctcccgc aaaaaatgaa
      721 cccgttgctt gttatagtgc cttgcttaat acattgcctg aatccggagt tactttttaa
      781 taattctgtt aatggttatt agctcaccta atatttcgaa gttatcaggt gggcagttaa
      841 tccatttgtt agagccagcg atgatttccc tgtcccagcg ttttattctt tcgccatttt
      901 tttctatgca tatcatgtaa tttgatgggg tcattaattc tttggatact gaaagacatt
      961 cccgaaatga tggtatttca agcaaaactt ccgcgcctga attttcacgg taaaaaatgt
     1021 gccatgttgt gcctttttta ttagttttca cgtgcgtatt ctcataggat aaatataaaa
     1081 ccttgtaaca ttcaggaggt aacaggattt attctgtaag aacaacagac agaacaatga
     1141 tatctgtttt atgtatacga tgtagccagt gatttagtac attgctggct atatcacttt
     1201 tcccattttg tgataagtct gctttgaaac gatggtgtcc ctcctcctgt attgtgaaag
     1261 ttgccacatt tttttcttca tatccctgaa catccccgtc gtgccttata taattgactc
     1321 cattaagaag ttgtcagaat aattatttct gattgcaacg gagttaattt cagtgatggt
     1381 aaagatatcc gtggcaccat aacgcatttt tcatcatcag tatagtggta tgcgtgaaca
     1441 tatatggtgg cattttgcct gatgttgtta aggcttttca gcaggtctac agtcaactcc
     1501 atcgaggtat ccttcaggtt gttatcattc gttgatgatg cgatctgttg aaggcgttgg
     1561 cgacaattaa ttctttttct cttctgaaaa atttacggaa tacaaaaaag atctgttttt
     1621 aactgattgt tactttctga agatattctc tgctccattt gctttccggg taagacgtat
     1681 ggctggttat ggtatggtcg gactgcagcc acagggggat gactgtaata cctctgatca
     1741 ccacagtcag tcatctgtaa atgttaaata cctcacagtt cagtcagtgg attattgtcg
     1801 gtcttacggg atatttgtta aaaaaatact gcatggtgaa tccccctgag cggcggggca
     1861 tatcagtatc gtaggtgttt ggtcacatcc cttatctgtg cgggttcagg tgctgatact
     1921 gaactcaccg ggaggcaccc ggcaccatgc tacataacac tccattacgc tacactcgcc
     1981 cctctccgga ggggttttct ctgcaggatg tgaatatgag atgctgcatc attcactgcg
     2041 aatattgatg gtgagaatct ttaat
//
LOCUS       DAZGTJ010000242         2022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000242 DAZGTJ010000000
VERSION     DAZGTJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.244"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..777
                     /locus_tag="ACHK8E_005212"
     CDS             <1..777
                     /locus_tag="ACHK8E_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0347311.1"
                     /translation="PSVTPQWVTRTNYAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTGYPDPRGELDPEADPGLLYAGQWQDTESGLCYNRFRYYEPETGMYLVSDP
                     LGLLGGEQTYRYVPNPCGYVDPLGLAITSIINKTVEFSRGPQTYVSDLGHVTGSTARA
                     RNKAITAILKEDFAYLKLTYIPQYNPFMRTGIAKLWEGTQFGKNSFSSRAALRDVIIH
                     EELHHRWWKRGLNDHHPKGTEMEQKFYETIRRYKRMRGWL"
     gene            777..1190
                     /locus_tag="ACHK8E_005213"
     CDS             777..1190
                     /locus_tag="ACHK8E_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347312.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWNVRLENIKSQMETIKVIRKAFSIDTKEALRII
                     RNESKVIYSGTREEAIWLMSLLKHVGETPLIQIAR"
     gene            1245..1406
                     /locus_tag="ACHK8E_005214"
                     /pseudo
     CDS             1245..1406
                     /locus_tag="ACHK8E_005214"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            1437..>2022
                     /locus_tag="ACHK8E_005215"
     CDS             1437..>2022
                     /locus_tag="ACHK8E_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP0347313.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
BASE COUNT          539 a          464 c          578 g          441 t
ORIGIN      
        1 ccgtccgtga ccccgcagtg ggtgacgcgg acgaattatg cggtgagcga cctgacaggc
       61 cgcccgctga tgctctttaa cagtgaaggt aaaaccgtct ggcggccggg acagaccagc
      121 ctgtggggac tggcactcag cctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gatccgctgg ggttgctggg gggtgagcag acttaccggt atgtgcctaa tccgtgtggg
      361 tatgttgatc cgctgggatt agctataact agtataatta ataaaacagt cgagttttct
      421 agagggccac agacttatgt ttctgatctc ggtcatgtta caggttctac ggccagagcc
      481 agaaataagg caattacagc catacttaaa gaagattttg catatcttaa actaacatat
      541 atccctcaat acaatccgtt tatgcgaaca gggattgcta aactttggga aggaactcag
      601 tttggtaaaa atagtttttc gtctcgtgca gcattaagag atgttattat tcatgaagaa
      661 ttacatcata ggtggtggaa gcgaggattg aatgatcatc atcccaaagg tactgagatg
      721 gaacagaaat tttacgagac gattcgaaga tataagcgga tgagagggtg gttatagtgt
      781 ttggtgatga atttaaaacg actattgagt gtccttgctg tggaaaaata tgttctatag
      841 ctatagggca gacgagtcac aatggtatac tcgcatggta tgaatcaatt atatgccata
      901 attgcggatt acgtagtgaa tcagatgata taggctttcc cccagaaaaa attcgtctac
      961 aattgattga ttttgagggt ttatggaatg ttagacttga aaatataaaa tcacagatgg
     1021 aaactataaa agtcattcgt aaggcttttt cgattgatac aaaagaagca cttcgaatta
     1081 ttagaaatga atcgaaggta atatactccg gaacgagaga agaggccatc tggttaatga
     1141 gtttattaaa acatgtgggt gaaaccccct taatacaaat tgctagataa attctatgag
     1201 ctgggtacaa cggagtagcc tggcgttacc gctatgacgc cgatggcctg tgtaccgcca
     1261 gggttaacgg tctggaggaa accatcctct acagccggga cgccgcaggc cgcctggcag
     1321 aagtcatcac gccggaaggc aaaacgcagt atgcgtatga caaatccggc aggctgacgg
     1381 gtatcttcag cccggacggc acatcacagc gcaccggcta tgacgaacgc gggcgggtga
     1441 atgtcaccac tcagggccga cgggccattg aataccacta ccccgatgaa cacaccgtca
     1501 tccgctgtat cctgccaccg gaagatgaac gcgacagaca tcccggtgaa tccctgctga
     1561 aaaccaccta ccgctacaac gccgccggag aactgacgga ggttatcctg ccgggggatg
     1621 agacgctgac gttcagccgt gatgaggcag gacgtgaagt gctccggcac agtaaccggg
     1681 gttttgcctg tgagcagggc tggaatgcag ccagccagct tgtcacccag cgcgccggat
     1741 ttttcccgga ggaaaccaca tggggcgggc tgctcccctc actggtacgg gagtaccgtt
     1801 acgacagcgc gggcaatgtg tcggctgtca ccagccggga agattacgga cgggaaacac
     1861 ggcgggaata ccggctggac cggaacggtc aggtcacggc ggtgacagcc tcaggcaccg
     1921 ggctgggcta tggcgaaggc gatgagtcct atggctatga cagttgtggc tacctgaagg
     1981 cgcagtctgc gggcaggcac cggataagtg aagagactga cc
//
LOCUS       DAZGTJ010000243         1937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000243 DAZGTJ010000000
VERSION     DAZGTJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.245"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..312)
                     /locus_tag="ACHK8E_005216"
     CDS             complement(<1..312)
                     /locus_tag="ACHK8E_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347314.1"
                     /translation="MLAISSNISKMVIFIFAIIIVVFLCVTTYLYLHKDESLVSKHYI
                     NYMAIPESDGVFTWLPDFFPHVAVDISISTNVEDDYFFSYFSLTIDDGGRFKKTLTVR
                     AR"
     gene            complement(293..>1937)
                     /locus_tag="ACHK8E_005217"
     CDS             complement(293..>1937)
                     /locus_tag="ACHK8E_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0347315.1"
                     /translation="LQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGYSATGRLA
                     SVRTLAPDLDISIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGR
                     LTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRTG
                     KRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDSTRIQTVYQPGSFTPLIRIE
                     TDVTELEKAQRRSLAEKLQQEGSEDGHGVVFPAKLVRLLDRLEEEIRTDRVSSESRAW
                     LAQCGLTVEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTVWSAEYDEWGNL
                     LNEENPHHVYQSYRLPGQQHDEESGLYYNRNRYYNPLLGRYITQDPIGLAGGWSLYAY
                     PLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKRLNLGGTDQFFHCM
                     AFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSEMIEDNKKDLAV
                     NEHGLTCPLTQDCSNRCIDYINPEHKKTIKALQDAGYLK"
BASE COUNT          420 a          567 c          427 g          523 t
ORIGIN      
        1 cctggccctc acagtcaatg ttttcttaaa cctcccccca tcatcgattg ttagggaaaa
       61 ataagaaaaa aaataatcat cttctacatt tgtggatatt gatatatcca ccgctacgtg
      121 cggaaaaaaa tctgggagcc atgtaaaaac cccatcactt tctggtattg ccatatagtt
      181 tatgtaatgc ttacttacaa gagattcatc tttgtgtaaa taaagataag tagtcacgca
      241 taaaaaaaca acgattataa tagcaaaaat aaatattacc atctttgaga tgttacttga
      301 gatagccagc atcttgtaaa gcctttatcg tttttttatg ctctggatta atataatcaa
      361 tacatctatt tgagcaatct tgtgttaatg gacatgtcaa cccatgttca tttacagcca
      421 agtctttttt attatcttca atcatctcag aatgggataa ctttactttt ctgccgtaca
      481 taccgaacat atttaacccg taatctctaa tctctttttc ataacccagc cctttcgctg
      541 atcggctaac accagcgtca tttaatttag acactcgaca aaatgccata caatggaaaa
      601 attgatcagt cccacctaaa tttaatctct tcatatcatc ataagtatca gataatatat
      661 cccatgctct ttgatggttt aattgttctt tttttcgcat taacgctaca tctgcaggac
      721 ttaatcctaa tggatcaata ccattcaccg gattcagcgg atacgcatac agactccatc
      781 cccccgccag cccaatcggg tcctgagtaa tatatcgtcc cagcaacgga ttgtagtacc
      841 ggttccggtt gtagtacagc cccgactcct catcatgctg ctgccccggc agacggtatg
      901 actgatacac atgatgcggg ttctcctcat taagcaggtt tccccattca tcatactccg
      961 cgctccacac cgtattgccg tcttcgctga taagcgccag cggcaggccc cggtggtcgc
     1021 agtggtaaag atgaactttt cgcgccggtg tatattccgg ctccacctgt ttttccagtt
     1081 gctccaccgt cagcccgcac tgcgcaagcc acgcccggct ttcactgctc acgcggtctg
     1141 tccggatttc ttcctccagc ctgtccagca gccgcaccag tttagccggg aacaccacgc
     1201 cgtgtccgtc ctcgctccct tcctgctgga gcttctccgc caggctccgc cgctgcgctt
     1261 tctccagttc ggtcacgtct gtttcgatgc ggatgagcgg tgtgaagctt cccggctggt
     1321 acaccgtctg gatacgcgtg ctgtcagtct gcaccgtcgt cagcctgtcg ccgtcccacc
     1381 cgtaccacgt cacctccggt ttacgcgaca gcgacatcca ccccgtcagg tcacgctccc
     1441 gccgccatac ccgttttccc gtccggcggc ccagcgggtc gtagaggtag cggctctcga
     1501 ccagtggctc gccatgctgt atccgcgtgt ggaacaccag acggtgctgg ctgtcgtagt
     1561 ggtagtggtg ggtccgctcg tcgtccgtcc gtatcacacc cgccgggatg cggtccgtct
     1621 tctccgtcag cctgccgtat tcatcgtggc ggtagacata gtgcgcatcc tccgcgatgc
     1681 ggttatccgg ccacactgtg agtgtactgt ccgggtgcag ctccgggtcc ggcagccggt
     1741 ttcccgccgg gtcggtggca tacgggatgc tgatatccag gtccggtgcg agggtgcgca
     1801 cactcgccag cctgcccgtg gcactgtagc cgtattcccg cgtctgtcgc gggccgctga
     1861 tgcgcatcag gtcgccgttg tcattccagt tatagtcaca atcaaacacc aggctgttca
     1921 ggtgccggct ctgtaac
//
LOCUS       DAZGTJ010000244         1872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000244 DAZGTJ010000000
VERSION     DAZGTJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.246"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..223
                     /locus_tag="ACHK8E_005218"
     CDS             <1..223
                     /locus_tag="ACHK8E_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347316.1"
                     /translation="NFLPKSRQKLLRSAPGQSFPAVAVMPHAAKVLKRVMVNTGGNKR
                     QVPAKRQNRGSRNSTGDGFKKTKPGSLTI"
     gene            394..528
                     /locus_tag="ACHK8E_005219"
     CDS             394..528
                     /locus_tag="ACHK8E_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347317.1"
                     /translation="MSLRQIVTLADAVVKGQPTRIPPMKGDEWREFCWWLDHLRGYAM
                     "
     gene            589..1071
                     /locus_tag="ACHK8E_005220"
     CDS             589..1071
                     /locus_tag="ACHK8E_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347318.1"
                     /translation="MTVVQNAVLRKTQLVFGAVAGIQELKSKRLQKKCRALAEREKPF
                     RIMEFTNFLNCGQHRLFVLRNVEGEIPHTGILPDVKAFSGGVITGKRFIGLIVVDRVE
                     EKILVYQYARSNWTDRKFEENIRISYDSPVFALKHFTKVNIWFWQRELHILLNKTRCR
                     "
     gene            1149..1550
                     /locus_tag="ACHK8E_005221"
     CDS             1149..1550
                     /locus_tag="ACHK8E_005221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347319.1"
                     /translation="MRKNEDNILFVPAGMTREDMVSEIALLRSSRDLTTDIYFEYVDA
                     GFPETTELSAADTGRLKAGLPAHHFHWLGADGAPLTVRRISDFADVADDFSCLAAAID
                     PELAEPPEEGIPVEGFNGGIFCVIREEDTDK"
     gene            complement(1604..1872)
                     /locus_tag="ACHK8E_005222"
                     /pseudo
     CDS             complement(1604..1872)
                     /locus_tag="ACHK8E_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          472 a          449 c          523 g          428 t
ORIGIN      
        1 aaactttttg cccaaaagca ggcaaaaact tttgcggtct gcccccggac agtcgtttcc
       61 ggctgtggca gtgatgcccc atgcggcgaa ggttctcaaa cgggtgatgg tgaataccgg
      121 agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa actcaacggg
      181 tgatggtttt aaaaagacaa aacccggttc tttaacaatc tgacaatgca atgaaaatca
      241 ctggattatg cagcaattaa ggaggtgaca cgatgtaggc gcaatacagc gcaagggaaa
      301 gcacggggag gaaaaggacg ttcacgggta tgcaaggggg tgtaggagca cccctttgca
      361 cgaatatcaa caacctttgt aaggatattg attatgtcac tcagacaaat cgtaaccctt
      421 gctgatgcgg ttgtcaaagg gcaacccact cgcatcccgc caatgaaagg cgacgaatgg
      481 cgcgagttct gttggtggct ggaccacctc agaggctatg ccatgtgact cccggggcgg
      541 taacgccccc ttttaaacgg tttcatttag cccctgacgg aggcgattat gacagtagtg
      601 cagaatgcag tgttgcggaa gacacagctt gtttttggtg ctgtggccgg aatacaggaa
      661 ttaaaaagta aacggcttca gaaaaagtgc cgggcgctgg cggagcgtga aaaaccgttt
      721 cgcatcatgg agttcacgaa ttttttaaac tgtggtcagc atcgtttatt tgtgttgcgg
      781 aacgtggaag gtgaaatacc gcacacaggg atactgccgg atgtgaaagc attttcaggc
      841 ggagtaataa ccggaaagcg gtttattggt ctgatagtcg ttgaccgcgt ggaggaaaaa
      901 atcctggttt atcagtatgc acgcagtaac tggacagacc ggaaatttga agaaaatatc
      961 cgaatcagct atgacagccc ggtttttgct ctgaagcatt tcacgaaggt gaacatctgg
     1021 ttctggcaaa gggaattaca tattctgttg aacaaaacca ggtgcagata aatccgggcg
     1081 gggcctgacg gtcccgccct tctgacgcag agcgggcgac accgtgatac tgaatgagag
     1141 acgaggcaat gagaaaaaac gaagataata tcctgtttgt gcccgcaggc atgacgcgtg
     1201 aagatatggt cagcgagatt gctctgctac gttcttcacg cgacctgacg acggatattt
     1261 attttgaata cgtggatgcg ggatttcccg aaacaaccga actctctgcc gcagacaccg
     1321 gacgcctgaa agccgggtta ccagctcatc attttcactg gttaggcgcg gatggtgctc
     1381 ccctgacggt aaggcgaata agtgactttg ccgatgttgc tgatgatttc agctgtctgg
     1441 cagccgccat cgacccggaa ctggctgagc caccggagga gggtatacca gtggaaggat
     1501 ttaacggggg gattttctgc gtaattcggg aagaggatac agataaataa ccctcaatgg
     1561 caacctcagg catccgccag caaaaatatg aaaggggccg cgctcacgcg ccgccccttt
     1621 cattacgcgg atgttctgat gtgggcctct gtcttgtcat ccctgtcatt tgcctgctcc
     1681 tgttactgat cttcttttcc ctgctgctct gattactgtc aggtctgttg ttctgatgct
     1741 tatgcctttc ccgggcgtcc ccctggcggg gcgggctgtc gtgaacggag ccagcgtagc
     1801 tgtctccgcc ctgacgggct tccatccctg acgcaaaacc ccgggtttgc agcgaccgga
     1861 acggtcgcgc ca
//
LOCUS       DAZGTJ010000245         1865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000245 DAZGTJ010000000
VERSION     DAZGTJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.247"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            17..394
                     /locus_tag="ACHK8E_005223"
     CDS             17..394
                     /locus_tag="ACHK8E_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347320.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            476..1105
                     /locus_tag="ACHK8E_005224"
     CDS             476..1105
                     /locus_tag="ACHK8E_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347321.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            1221..1604
                     /locus_tag="ACHK8E_005225"
     CDS             1221..1604
                     /locus_tag="ACHK8E_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347322.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            1673..>1865
                     /locus_tag="ACHK8E_005226"
     CDS             1673..>1865
                     /locus_tag="ACHK8E_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0347323.1"
                     /translation="MFFRGHALMKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVP
                     PWKRPWRSARNGVGLPEMMP"
BASE COUNT          384 a          439 c          549 g          493 t
ORIGIN      
        1 agagacggag gtcatcatgt attcaccttt aattgctcat tattcagcgc ttcagacgca
       61 gtctgctctg ctggctcata tcagtcagat ggaaggcgag ctgatgcggt tacgtgcctg
      121 gcaacggctg ggtattaccc cggagccgga tgatgagacg gagccttcac tggtatacgt
      181 gtatctgtgg gataccggcg aggcgctggg ctggctgttg tatgacctgg agcaggagga
      241 gatttccgca ccgggaattc aggcccgtca ggcactcgac agtctgatgg ctctggggcg
      301 tgaaattaat catgaaatct gggctgacag tatcagtacc ggaaagatgg tggccggagc
      361 ggatgaatat tttgcccggc acgacatgat gtaattcatg acctgaccct gacgggggag
      421 ttctccgtcc gggagacatt ccccgttgtt tcgttttaac catgaggaat gttccatgtc
      481 tgagttatct tcacgtccgg cccgtgagcc ggtggtatat acccttgagc aggtttccac
      541 aataccggaa aagcagtggc atgcttttgt tctggctgtc actgaaacct tctggcagtt
      601 gccggaggcg ttacggccgc agaatgcgta tttcggctcc ctgacccggg cgtcagagtt
      661 gtttccggta acggacacgc tggcgtttta ctgccggtct gccgacgggc tgtggtcggt
      721 gaatgtcact attgagcggg agcacagccg gaatatcctg gctcttaatg agctgaattt
      781 cggacgtcag ccgggagatt tctttgcccg tacagtcttt gtgctgttgc acaatctgtg
      841 tccggactgt tttcgtattc attccactgt cggtggtgcc agctggtcgc tgccgctgaa
      901 atggataaaa cggtacctgg ggcatgagaa tttttctgct ccggagtctg tattaaccac
      961 cccggtaagg ggagatgcgt ttgacagctt actgctgcag tttttgtccg gtcaggggcg
     1021 acagttatcc ccggatgact gggcagcact ggaagaggcc gaatatcagc tttactggct
     1081 gcgggctctt gccggtgggc ggtaacagaa cgccgtagtt tctttatccg gaataacgtc
     1141 atcacgtatt ccgtcattat atcacccggc agggacatat ccctgtgggg agagtcccgg
     1201 ctttattcag gagaattgtt atgggactcg atatttactt tctggctcgt gataaccatc
     1261 acagtggtgc cgttgtggga acacaggaac atcaggaggt gggggctttc cgtaaggtga
     1321 aaccgcttct gacatggttt cgtgaacata ccggtgcttc agatgagcag catgctttcc
     1381 ctgtaacccg cagttcactg gctgcattat tgcgcgacct cagtatgctg acaccgggga
     1441 actgccacag gctgtttcct tatgtcacag gctcgtgttt acggggggaa gattatgccg
     1501 gtgattactg gtatgacgtg gatgacgtaa aagcgtgggt cagcaatacg ctgaagacgt
     1561 ttgattttga gcgtcgttca ctggtgatgc tggcgttgtg gtaattctgg ccagtctgat
     1621 tttcgttatt aatttaactg tacggtgcac tctcaccgta cagggagcgt gcatcttttt
     1681 cagggggcat gctcttatga aaacgaaaaa acatacttca cgtaccgcga ctaaaactcc
     1741 ggaacgtgac ctgtatcagc ttgtgaccga cagggttgtt acggccattg aaaatggtgt
     1801 gccaccctgg aaacgtccct ggcgctctgc gcgtaatggc gtggggcttc cggagatgat
     1861 gccgg
//
LOCUS       DAZGTJ010000246         1843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000246 DAZGTJ010000000
VERSION     DAZGTJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.248"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHK8E_005227"
     CDS             complement(<1..332)
                     /locus_tag="ACHK8E_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0347324.1"
                     /translation="MERESQPARTGLTQTLALNNDIWRVSFYRFCQLLELENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQR"
     gene            complement(296..>1843)
                     /gene="tssF"
                     /locus_tag="ACHK8E_005228"
     CDS             complement(296..>1843)
                     /gene="tssF"
                     /locus_tag="ACHK8E_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0347325.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAGSTWFEIDIVLSEAWSSGLPFET
                     ENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVMNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HNLHVPG"
BASE COUNT          414 a          512 c          531 g          386 t
ORIGIN      
        1 cgctgcgtaa tatcatccag ccaggacgtc ggcagaggtg agtccacccc atacattccg
       61 agaaatgtcg tgcggattgt tgggggtaat gtcggacggt cttcgtcgat ctccaccgct
      121 ttcagggtgc tgaccgggaa tcccatcccg ggccagggtc tgaatcgcac cggatcatca
      181 cccggatggc tggtcgtgcc cagttttggc ccgtccggat tttccagctc cagcagctgg
      241 cagaaacgat aaaaactgac ccgccagata tcgttgttaa gagccagggt ctgcgtcagc
      301 ccggtacgtg caggttgtga ttctctttcc atctcagtcg tttccctgtg ggttgcagta
      361 ccagtgtgag ctggttgaaa aggtggacat cagcgtagag cgcaaagaaa cggtgcagca
      421 tctccccgaa gagattcaca tcgccctcgc cggcaaaatt atccatattc agcgtgacct
      481 caatgtccac gccacggagc ataaagccct tttcaaaacg gcggatcagg gtgtgcttca
      541 cggcaacgat ggcttcaagc cggcggcggt tcatttcatc gtcggtccag tcatagagcg
      601 ccagcgtccc ccgcagcacc tccggattgt ccatcatgct gagaaagttt gatccgaggt
      661 gactcatcac ccgccagtga aaccggtcat ttgccggagg atacagtggc atcgtcggtg
      721 cggtgaggtt catgactctg acggggactt tgccggtttt gaccaccctg tccaggagcg
      781 tactttccag cgccctgcgc ggaagctgcc cgttggtggc ggtgattcgc atcgagaggg
      841 actccggttt atcagacagc tgctccagtt cgaacgagcg tcccccgaga actaaccatg
      901 tatcatacag accggagggg ccccgtttca cgcgggtgtg gtaataacgt tcaggagcat
      961 cgtggcgcat cataccgccc cgatgccgga agctggtgaa cgggacataa gggtgtctgc
     1021 cattcttcac cgagccgtga atgttatcga cgctgtaaat ttcggtgtga ccatcctgaa
     1081 gacgcagcgg cctgagcagg tactcgttat ccagcgggtt aaggatcagc gggtcggctt
     1141 ccagggtaaa gagattgata accggcgcac agtgcaggcg gaagttttct gtctcaaaag
     1201 gcaaaccgga tgaccaggct tcactgagca caatgtcgat ttcaaaccag gtactgcccg
     1261 ccgtgagccc gatggtgtcg aggccgcgca gttcaacaaa cataaacttc ggacggaagc
     1321 tgaaatactc cagcagcaac tggtaaccac tgaaggcagt atcggctttc ttccacaggc
     1381 agtcattgtc gtcaaagccc atgggtctgc accagccgtc aaactgatga cgtccggtat
     1441 atgtcccggc atgacgcgca tacatggcat gtacacgccg ggtcattgcc agatgcagag
     1501 cagcgcttac cggcgcctct gcgttaagaa aaatggcgac tttgtcgatc ccggctttac
     1561 tccagtccac tttttcaggg cattcgaagc gcaggcggat agcagagcgg ccatcggatt
     1621 cagtatggag acgggcatct gcaaggtgca gcggttgtag caacacatcg cgggtcgtcc
     1681 ggtactgaca ggcggtttta tgcggcccga caggacggga caaaacggag aagccttcag
     1741 ccagtatttc ggcctgacgc agactacgcc agtcaggcga gaactcaacg atggccagcg
     1801 acggaatagt ccgcatatag tgcggccaca gcaggctcac cag
//
LOCUS       DAZGTJ010000247         1777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000247 DAZGTJ010000000
VERSION     DAZGTJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.249"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..776
                     /gene="bluR"
                     /locus_tag="ACHK8E_005229"
     CDS             45..776
                     /gene="bluR"
                     /locus_tag="ACHK8E_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP0347326.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            997..1401
                     /locus_tag="ACHK8E_005230"
     CDS             997..1401
                     /locus_tag="ACHK8E_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0347327.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            1454..1564
                     /locus_tag="ACHK8E_005231"
     CDS             1454..1564
                     /locus_tag="ACHK8E_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347328.1"
                     /translation="MEIKVQRLSLWMINTVFLLPPINNHQANTINLIFEM"
BASE COUNT          533 a          378 c          372 g          494 t
ORIGIN      
        1 ggtcgtgtac aggcgacgga gatttgtgac cgcaaggagg aattgtggct tattacagca
       61 ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc tggcaacgcc
      121 gctacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt gatgaagaag
      181 acatacaacg catcgaagag atcaagcgtt ggataagtaa tggcgttcct gtaggcaaag
      241 ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagt cgcctgcaag
      301 aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg agaattattt
      361 cactgggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt cctgttcgcc
      421 agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt gacggcgcat
      481 taattgaata cgcagcagcc tcacttttcg aaatgcgccg taagcccggt aaagaagcca
      541 ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagca tggcgtttat
      601 cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgccg cgcccggaac
      661 tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga aggcaaaacg
      721 aacttttaca acactggggt gagcaaggtt ataaagtcat ctttcatgcc ccctaatttc
      781 acctttatcc cgccccttag acagaatctt ataatctgaa cccacaacag cttttgtacc
      841 ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag gtaatccccc tttcacactt
      901 tgtacatatc acagccaaag gacagaccag attgaagtgg taaaaccgat cgtgcacact
      961 acgcagtctc acaactaaca agaggttatt atcctgatgg atcaggtcgt tgtttttcaa
     1021 aagatgtttg agcaagtgag aaaagagcaa aacttctcct ggttttattc agaattaaaa
     1081 catcaccgcg ttgcacatta catttattat ctggctacgg ataacatcag aattattact
     1141 cacgatgaca cggttttgtt attaagagga accaggaacc tgttaaaagt tagtacgaaa
     1201 aagaaccctg ctaaaataaa agaggccgca ttgcttcata tttccggaaa atctacattt
     1261 cgggagtact gttcaacact ggcagacgcg ggcgttttcc ggtgggttac tgatgttaat
     1321 cataacaaac gcagttacta cgccattgat aatacgcttt tatatattga agatgtagaa
     1381 aataataaac cattaatcta gctaaagttg gatgcttaaa aaatgcttca taattcagta
     1441 agacattagc ataatggaaa taaaagtgca gagactatcc ctatggatga taaatactgt
     1501 ctttttattg ccacccataa ataatcacca ggccaatact atcaacttga tatttgaaat
     1561 gtgatcactt gactttctat acgttatttt ataacggtta acatatttat aaaaccaacg
     1621 ggcgtgccat acgcccgttt caatacttaa cgcacatgtg ttttggttta gtcatcatcc
     1681 gattatatgt attttagtca ggaacgggtt taaccattcc tatataactc aaaaattgaa
     1741 accttattct catgtcatgc ttatattcat tattatc
//
LOCUS       DAZGTJ010000248         1750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000248 DAZGTJ010000000
VERSION     DAZGTJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.250"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1750
                     /locus_tag="ACHK8E_005232"
     CDS             <1..>1750
                     /locus_tag="ACHK8E_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0347329.1"
                     /translation="VSEKSTDAINGSQLYAIAKTLKDDLDAINPTWKLKDSTGKEKEV
                     GSKNGGTTGNEVTVTGKGDIDVTVDGNGLSIDGTKLAENIKKDAIKIKTGDGKTLTKK
                     LGEEVEFNDAGPIKTKINNNKLEITAETASFTPDGNGGNGKITVSNGDDDKLVTAKNI
                     ADAINQSGWKIKAGSENGGKVEGDNKEELINPGDTVTLKAGKNLSVKQSNGDFTFSVV
                     DTPSFSSVQLNGKGNNGNVTLSSEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTL
                     TNTDKEMKNAASVADLKTAIDGVSTSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNI
                     TTTAITNGDKGLKISLNNDLSVGSNKAPGSIKVNGTDGKEAVSLNGQDGTIKLTSAPA
                     ANGKSASATIAVNAGKGGLTDAVGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGK
                     IIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAVFGTEN
                     GPTTRIDNAGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGD
                     VLNNAANIGDLKNALRD"
BASE COUNT          570 a          319 c          478 g          383 t
ORIGIN      
        1 gtatctgaga aaagtacaga tgccattaac ggtagtcagt tatatgcgat agcgaagaca
       61 ctgaaagatg accttgatgc cattaatccg acgtggaagt taaaagacag cacaggaaaa
      121 gaaaaagagg tcggcagtaa aaatggcggg actacaggta atgaagtgac cgtcaccggt
      181 aaaggggata ttgatgttac tgttgatgga aatgggctga gtattgatgg cacaaagctt
      241 gctgaaaaca tcaaaaaaga tgccatcaag attaaaacgg gagatggtaa aacactaacg
      301 aaaaaacttg gtgaagaagt tgagtttaat gatgccggac caataaaaac aaaaattaat
      361 aataacaaac ttgagataac tgcagagaca gccagtttta cgcctgatgg aaatggtggt
      421 aacgggaaga ttacagtaag caatggtgat gacgataagc ttgttactgc gaaaaatatt
      481 gctgatgcca taaaccagtc tggctggaaa attaaagctg gcagtgaaaa tggtgggaaa
      541 gttgaggggg ataataaaga ggagctaatt aatcccggtg atacagttac actgaaagcc
      601 ggtaaaaatc tcagcgtgaa acaaagtaac ggtgatttta cattctctgt tgttgatact
      661 ccgtcattca gcagtgttca gctgaatgga aaaggaaata acggaaatgt aacgctctca
      721 tccgaaggta ataagctgac attgtcaggc ggtaatggtg ccggacaaaa tggtgttgtt
      781 atcagtggtg tggcaagtgg actggaaggt aaggaacttt cgacgttaac gaatactgat
      841 aaagagatga aaaatgccgc cagtgttgca gacctgaaaa cggcaattga tggtgtgtcc
      901 accagtatgc agggtgcagg tttcgcactg aaagactctg atgataagga agtgaaacag
      961 tctcttgggc aggcgattaa agtatccggg gacagcaata tcaccaccac agcaatcacc
     1021 aatggggata aggggttaaa aatatccctg aataacgacc tgagcgtggg gagcaataag
     1081 gctcccggca gcatcaaagt taacgggact gatggtaagg aggctgtgag tctgaatggt
     1141 caggatggca cgattaaact gacatccgcg cctgctgcga acggaaagtc tgcgtcagca
     1201 accattgctg ttaatgcagg caaaggtgga ttaacagatg ctgtcggtac cacgaagaca
     1261 cgtctgactt ataccccgga tggaaaatca ccggcagaag agctggcgac catgaatgac
     1321 ggtctttctt ttgagggtga tgacggaaag attattcata aaaaactgaa tgaaaaactg
     1381 accatcaaag gtggtgctgg tgataatgag gatgtaacag acagtaacat tcgtgttgat
     1441 gcaaatgaaa agggagatgg tcttctcgtc cggatgacca gaaacctccg tgatttggcc
     1501 agcgcggttt ttggtacaga aaacggcccg acaacccgga ttgacaatgc aggcatcaca
     1561 atcacaacac ctgctgcggg cggtggtcag gggaatacgg tttcccttac cggctccggc
     1621 ctgaataacg gtggaaatca gataaccagt gtggccagtg gtctgaacgg cactgcgctt
     1681 gctgacgccg gtggagatgt tctgaataat gcggccaata tcggtgacct gaaaaatgct
     1741 ctccgtgaca
//
LOCUS       DAZGTJ010000249         1747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000249 DAZGTJ010000000
VERSION     DAZGTJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.251"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1747
                     /locus_tag="ACHK8E_005233"
     CDS             <1..>1747
                     /locus_tag="ACHK8E_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0347330.1"
                     /translation="ISDTSTDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATI
                     NGGKDNKNGNTLTVKGDGDIAVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKS
                     LGDNVEITGKGPIKTQVAGDKLEISAETASLPVNGGNGKITVNNGDGDKLVTAKNIAE
                     AINQSGWKIKADKDTGGVIEGNGQGTETLIKPGDMVTLKAGKNLSVKQSGGEFTFSVI
                     DTPSFSSVQLNGNGKNGNVTLSSEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTL
                     TNTDKEMKNAASVADLKTAIDGVSTSMQGAGFALKDSDDKEVKQSLGQSIKVSGDSNI
                     TTTAITNGDKGLKISLNNNLSVGSNNTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPA
                     ANGKSASATIAVNAGKGGLTDAAGTTKTRLTYAPDGTLPAEELATMNDGLSFEGDDGK
                     VIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAVFGTGG
                     QITTVNDKGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDV
                     LNNAANIGDLKNALRD"
BASE COUNT          556 a          317 c          479 g          395 t
ORIGIN      
        1 atttctgaca caagtacaga tgccattaac ggtagtcagt tatatgcgat agcgaagaca
       61 ctgaaagatg accttgatgc cattaatccg acgtggacgc taaaggatga taagggaggt
      121 tctgcgacta ttaatggtgg gaaggataat aagaatggta acactttaac tgttaaaggt
      181 gatggagata ttgcagttac tgttaatgat aaaggaatga cgattgacgg aacaacgctg
      241 aaaaatgaaa taaaaaatga tgccatcaaa attaaaggtg gagatggtaa gacgctgacg
      301 aagagtcttg gtgacaatgt cgaaatcact ggcaaaggcc caataaaaac ccaggtggct
      361 ggagataaac tggagatttc agcagagaca gcttcgttgc ctgtaaatgg tggtaacggg
      421 aagattacag taaacaatgg tgatggcgat aaacttgtta ctgcgaaaaa cattgctgaa
      481 gccataaacc agtctggctg gaaaattaaa gcggataaag ataccggtgg tgttatcgag
      541 ggaaatggtc agggaacaga aacactgata aaacccggtg atatggttac gctgaaagcc
      601 ggtaaaaatc tcagcgtgaa acaaagtggt ggtgaattta cattctctgt tattgatact
      661 ccgtcattca gcagtgttca gctgaacggt aatggaaaaa acggaaatgt aacgctctca
      721 tccgaaggta ataagctgac attgtcaggc ggtaatggtg ccggacaaaa tggtgttgtt
      781 atcagtggtg tggcaagtgg actggaaggt aaggaacttt cgacgttaac gaatactgat
      841 aaagagatga aaaatgccgc cagtgttgca gacctgaaaa cggcaattga tggtgtgtcc
      901 accagtatgc agggagcagg tttcgcactg aaagactctg atgataagga agtgaaacag
      961 tctcttgggc agtcgattaa agtatccggg gacagcaata tcaccaccac agccatcacc
     1021 aatggggata aggggttaaa aatatccctg aataataacc tgagcgttgg gagcaataat
     1081 actcccggca gcatcaaagt taacggggct gatggtaagg aggctgtgag tctgaatggt
     1141 caggatggca cgattaaact gacatccgcg cctgctgcga acggaaagtc tgcgtcagca
     1201 accattgctg ttaatgcagg caaaggtgga ttaacagatg ctgccggtac cacgaagaca
     1261 cgtctgactt atgctccaga tggaacatta ccggcagaag agctggcgac catgaatgac
     1321 ggtctttctt ttgagggtga tgacggaaag gttattcata aaaaactgaa tgaaaaactg
     1381 accatcaaag gtggtgctgg tgataatgag gatgtaacag acagtaacat tcgtgttgat
     1441 gcaaatgaaa agggagatgg tcttctcgtc cggatgacca gaaacctccg tgatttggcc
     1501 agcgcggttt ttggtacagg tgggcagata acaacagtta atgataaagg catcacaatc
     1561 acaacaccgg ctgcgggcgg tggtcagggg aatacagttt cccttaccgg ctccggcctg
     1621 aataacggtg gaaatcagat aaccagtgtg gccagtggtc tgaacggcac tgcgcttgct
     1681 gacgccggtg gagatgttct gaataatgcg gccaatatcg gtgacctgaa aaatgctctc
     1741 cgtgaca
//
LOCUS       DAZGTJ010000250         1732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000250 DAZGTJ010000000
VERSION     DAZGTJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.252"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1732
                     /locus_tag="ACHK8E_005234"
     CDS             <1..>1732
                     /locus_tag="ACHK8E_005234"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP0347331.1"
                     /translation="HRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKRDKNSFD
                     FTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQIAATER
                     ILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVFFVVDRK
                     DLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKAESDLPV
                     YNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPIFPENASGSETTASVFGR
                     ELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQ
                     EITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAANKSANYK
                     PLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQMNFSTD
                     SKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGLMQAFSR
                     TNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGFTDAATG
                     EAKRGFMAVVS"
BASE COUNT          518 a          394 c          388 g          432 t
ORIGIN      
        1 catcgttaca gcaaggagag ttttaacagc gaaaattccc tgtttaagta tctgcaactg
       61 ttcgtcattt ccaacggtac cgatacccgt tactttgcca acacgacaaa gcgcgataaa
      121 aacagttttg acttcaccat gaactgggcg aaatcagaca acacgctgat taaagacctc
      181 aaagatttta ccgccacctt tttccagaaa cataccctgc ttaatgttct ggtgaactac
      241 agcgtttttg acagcagtca gacgctactg gtgatgcgac cgtaccagat tgctgccacc
      301 gagcgcattc tgtggaaaat taagagctcc tttacagcga agaactggtc aaaaccggaa
      361 agcggtgggt atatctggca caccacgggt tccggtaaaa cactgaccag ctttaaagcc
      421 gcgcgtctgg caacagaact ggactttatt gataaagtct tctttgtggt cgacaggaaa
      481 gacctcgatt accagaccat gaaggaatat cagcgttttt cgccagatag tgttaacggc
      541 tcggaaaata ccgcaggcct taaacgaaat ctggataagg acgataacaa aattatcgtc
      601 accactattc agaaacttaa taacctgatg aaagcagaaa gcgacctgcc tgtatacaat
      661 cagcaagtgg tgtttatatt tgatgaatgc caccgcagcc agtttggaga agcgcagaaa
      721 aatctgaaga agaaattcag acgctattat cagtttggtt ttaccggcac acctattttc
      781 ccggaaaacg cctcaggttc agaaacgacc gccagcgtat ttggtcgtga attgcattcg
      841 tatgtgatta ccgatgcgat tcgtgacgaa aaagtgctca aattcaaggt ggactacaac
      901 gatgtgcggc cacagtttaa gtctttagag acagaaactg acgagaaaaa actgagtgcg
      961 gctgaaaatc agcaggcgtt tcttcatccc atgcgtattc aggaaatcac gcaatatatc
     1021 ctgaataact tccgccagaa gacccaccgc actttcccgg gttccagagg ttttaatgcc
     1081 atgctggcag taagcagcgt ggatgctgcg aaagtctatt acgcgacgtt taaacgctta
     1141 caggaggaag ccgctaataa atctgcaaat tataaaccac tgcgtattgc gacaatattc
     1201 tcctttgccg ccaatgaaga acaaaatgcc attggtgaaa tttccgatga aacttttgat
     1261 accggcgcaa tggacagcag tgctaaagag tttctggatg ctgcaattcg tgaatataac
     1321 gaccattttc aaatgaattt cagtactgat agcaaggatt ttcagaacta ctatcgggac
     1381 ctggctcagc gggttaaaaa tcaggatgtt gacctgttaa tcgtcgtggg gatgttttta
     1441 accggcttcg atgctccaac actgaacacg ctgttcgtcg ataaaaacct gcgttttcac
     1501 ggtctgatgc aggcgttttc ccgtaccaac cgcatttatg acgccacgaa aaccttcggt
     1561 aacatcgtca cattccgtaa cctggagcag gcaaccatcg atgccatcac actttttggt
     1621 gataaaaaca ccagaaatgt ggtgctggaa aagagttatg cagagtatat ggaaggcttt
     1681 acagatgctg ccactggcga agctaaacgc ggctttatgg cagtagtttc ag
//
LOCUS       DAZGTJ010000251         1694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000251 DAZGTJ010000000
VERSION     DAZGTJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.253"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            46..426
                     /locus_tag="ACHK8E_005235"
                     /pseudo
     CDS             46..426
                     /locus_tag="ACHK8E_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            357..584
                     /locus_tag="ACHK8E_005236"
     CDS             357..584
                     /locus_tag="ACHK8E_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0347332.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            653..1063
                     /locus_tag="ACHK8E_005237"
     CDS             653..1063
                     /locus_tag="ACHK8E_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347333.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     HVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            1060..>1694
                     /locus_tag="ACHK8E_005238"
     CDS             1060..>1694
                     /locus_tag="ACHK8E_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0347334.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATTFSTALERACD"
BASE COUNT          389 a          514 c          450 g          341 t
ORIGIN      
        1 actgacgcag tgctctgtga actgttaccg ggctgagtga tcatcatgca cacacaaaaa
       61 aaccgcttgc cagcgttcgc ctggtcgggt tacagttttc ccgcacctca taaaacgggt
      121 gccgggatta ccaccccgcc gactacaaga gcgcacaacc gcgccagcgg tttttttgtg
      181 cgtactgtat cgccacgtct ttttcgcgtc agaattatgg cggggcgtac ggggccgact
      241 tcggtcgggc cgggatctct tgtagccggt tgtggtaacc ctgtacgtct cgccaccctg
      301 agattaccgc ctcgggttgg tgagttttca gaacttacta caagaggcca aacaccatgg
      361 caaaccgcaa acaacagcgc gcccgcgctg agcgtcttca catccgcact gaaattaacc
      421 gcagactcca tcgggctgca tctgtcacct tcatcatgca gtcaaacgca ttacatcgac
      481 ttaacgatgg tatcgctgcc gggtattacg cggcagtctt cagctatctg gccgaagacc
      541 tggcagaact gcaggacctt atcaaccagc aacaccacca ctaaacaaca tcctgaagcc
      601 atcccgcacc cgtgcgcggg aggcttccgc acgtctgtta ccggaggttc tcatgaagag
      661 cgttaaggga ctttcagata ttcaggattt ttttaactgg atgtgggcag tggatccccg
      721 gctggcttcc cgcctgaacg actggcacga ctattaccgc cgcgcctgta aacaggccgc
      781 cagacacgtc accacatacg gggatttctc cttcaccgtt gacgaccgtt accgcgtgga
      841 cgtgaaaggt gatggttttg gtttatttca catcaacgga accgacagca ccgccatcag
      901 ttggtcgtcg tcagagatga cgcttgttct ggaaatgaca aaccacagcg tggccgtgaa
      961 aggtatatac accgcagaag actttatcgg tgaataccag cgccttctcg tgctgtaccg
     1021 cgcggcgttt caggcggcga ctgccgggga gtgcgacgca tgagcgacca taatgcacaa
     1081 ctggcgtacc cgtggaacgc cccgctaccg gttatcgatc ctgaaacgtt cggcagagcg
     1141 cagccaaccg cattacgcca ggccattcag agctacatca acgaggatat tcgcactgac
     1201 gccagactgg atgaagaaac cgtcgccttc ctgacaacca cagacgaagg gaagcgaatt
     1261 aattcgcatc tttaccacga cgaagagcgt cggatcaggc tggaaaaact ggcgcggcac
     1321 aaacacgaga atccgccaac actggtatct gaagccatgg cagagctgcg cgccctgccg
     1381 ccgtttcttg ccggtccgct catccgtgac ctggcgcgcc tgagccgcaa acaggagagc
     1441 gcacgccggg aagggcttaa gaataacagc gcatatgcgg cggataactt cgttcgtcac
     1501 gggctgcgtg cccgcctgaa acgtatcgcc cgtgtgaatg aacgctttgc caccccggcc
     1561 tttaaggcca ccgccgcgcg cgaacggctg gatgaactgc ttatgctgcc acagctgagc
     1621 cgtgacgaaa tccagcgcct tgccaccctg accgccacca cgttttccac cgcactggag
     1681 cgcgcatgtg atga
//
LOCUS       DAZGTJ010000252         1617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000252 DAZGTJ010000000
VERSION     DAZGTJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.254"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..183)
                     /gene="tnpB"
                     /locus_tag="ACHK8E_005239"
     CDS             complement(<1..183)
                     /gene="tnpB"
                     /locus_tag="ACHK8E_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0347335.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTG"
     gene            complement(180..560)
                     /locus_tag="ACHK8E_005240"
     CDS             complement(180..560)
                     /locus_tag="ACHK8E_005240"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0347336.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(714..971)
                     /locus_tag="ACHK8E_005241"
     CDS             complement(714..971)
                     /locus_tag="ACHK8E_005241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347337.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            complement(1145..1360)
                     /locus_tag="ACHK8E_005242"
     CDS             complement(1145..1360)
                     /locus_tag="ACHK8E_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0347338.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
BASE COUNT          389 a          368 c          382 g          478 t
ORIGIN      
        1 accggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
       61 gccggacatg ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagcc
      121 atttctcata tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat
      181 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      241 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttc cggggcctct
      301 gcgagcgatt ctggctgttc ggcagcggca tcgagagtca caggaagtag ctgggggctc
      361 tctgaagaag gtaatagcag ttttccctcg cgccattgtt gtcgccattt gaacaacaga
      421 ttggcgttaa tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag
      481 gcagcaacga gctgctgttt aaattcggga ggataattag ggcagccttt tcgcctgccg
      541 ggagtcacat ttttctgcat atctgatact ttggttccca ctacttattt ggtggacacc
      601 actttgtcta attcgtcaga ttctgaccag acggttcagg ctgtacgctt actttccatc
      661 ccccatccag ctttttattg cctgactaac cagagggctg aatctaggaa tggctaacaa
      721 gcgtttgcac ataggatctt ggcggacata tttttcagtt cagaatcata ccactccagt
      781 tcattatcta gtgtaatcaa ccgtatatac tgccgatgca ataacgtctt catggtatca
      841 gtgagttcgt tttccgcatc ctcaagtaat gccggtaatt tttggcgtaa ttctgttgcg
      901 cctttactga acacaatgcc atattcagca agaagtccac gaacgtgatt tatcagttgt
      961 gtcctttcca tcgaaaccaa ccggcgcatt ttcaaaaaag tctggacgtc ctgttactcc
     1021 agagttttta ttggtacggg gcgtatagtt ccatgttggc aggcctcagc tattgcctga
     1081 gcgtcgtcat aatcattttt ctggcgtcgc tgataagctt taacatgttg cgccggaaga
     1141 aatattacat catggtcgag tttcctgatt tccctggccc agtgatggga tgcccgcgag
     1201 cctccatcgc aacagtcatt ttaggctgct gcaccagcca gttaagcagt tgattgcgag
     1261 aaagtttgat ccttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
     1321 ttttagccag atcgatgccg gtgattttct gttgagacat ggtatccctc ttacttttgt
     1381 ggttaaagtt tatccatcat gacgtaatta aaggttgtcg tgaaggaggg ggaagaccat
     1441 ctcatcgctt actgatgttc ctgttactat atctattgaa tcctggctat aagttcgctg
     1501 tttaaagaat tatatgaata gcaccaatta ataagttaat attaaaaaga agacagacct
     1561 tctaaataaa cgcattgaat ctatgttccc ttgtgagtgt ttttacgttt ggcggga
//
LOCUS       DAZGTJ010000253         1563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000253 DAZGTJ010000000
VERSION     DAZGTJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.258"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            155..826
                     /gene="queE"
                     /locus_tag="ACHK8E_005243"
     CDS             155..826
                     /gene="queE"
                     /locus_tag="ACHK8E_005243"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP0347339.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1166..1438
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1166..1194
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          413 a          339 c          370 g          441 t
ORIGIN      
        1 tatttatttc tgacataata cgttactccc ttttattatt aacgatctgt ttctcatttt
       61 tctttaaacc tgcctgtaac agaatgttaa taagacatga ttgttgtggc aggatctgca
      121 ataattaaca attatccact tcacagagaa tgctatgcag tacccgatta acgagatgtt
      181 ccagaccctg caaggtgagg gttactttac cggcgtcccc gccattttta ttcgtttaca
      241 gggatgcccg gttggctgtg cctggtgcga caccaaacac acctgggaaa agcttgagga
      301 tcgggaagtc tcccttttca gcattctggc gaagaccaaa gagagtgata agtggggggc
      361 ggcgagcagt gaagacttgc tggctgtcat tggtcgccag ggatacaccg cgcggcatgt
      421 ggtgattacg ggtggtgagc cttgcattca tgatttgcta ccactgactg atctgctcga
      481 aaagaatggt tttagctgcc agattgaaac cagtggtact catgaggtac gctgcacacc
      541 gaatacctgg gttaccgtat caccaaagct gaacatgcgc ggcggctatg aagtgttgtc
      601 acaggcactg gagcgagcca acgaaatcaa gcatccagtg gggcgcgtac gtgatattga
      661 agcactggat gaactactgg caacgctgac cgatgataaa ccgcgagtca ttgcactgca
      721 gccgattagc caaaaggatg atgccacacg tttgtgcatt gaaacctgca ttgcgcgtaa
      781 ttggcgtttg tcgatgcaaa cacataaata tctaaatatt gcctgattaa acatttataa
      841 gcgttataaa tgggtggaac ctgtaaagac ttctactcat ttatattgtt tgtcgcctct
      901 gaaaactcat ccatcttgcc catcccggac taatcattat cattctctac aaattctgtg
      961 gcgttaattt ttcgctggag tgaaaattat tgcggcgaag tttggtagat tttagtttgt
     1021 atagagttat tttaaatatt tagcttttta atcaatggat taagtgctct ttaacataat
     1081 ggatgtgttg tttgtgtgat actgtaaagt tggtagattg tgactggctt aaaaaatcat
     1141 taattaataa taggttatgt ttagagagtt ccccgcgcca gcggggataa accgagattt
     1201 cagaggcagc cgccaggccg ttgatcgagt tccccgcgcc agcggggata aaccgccatt
     1261 tcggtagcgc catcaaaatg gttacgcgag ttccccgcgc cagcggggat aaaccgttga
     1321 tcgccgtggc ggtgagatta gaaacgcgga gttccccgcg ccagcgggga taaaccggaa
     1381 gtggcaaagc tgccgtttct tttctctatg agttccccgc gcctgcgggg ataaaccacc
     1441 atataacccg ttatctcttt ctcaagtttt tatattagct gtacttgtaa taagcaacat
     1501 atccacgtaa cacctcatgt tcaaaatagt tctccatgcc agagaagttc acaattatcg
     1561 ata
//
LOCUS       DAZGTJ010000254         1535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000254 DAZGTJ010000000
VERSION     DAZGTJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.260"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..92)
                     /locus_tag="ACHK8E_005244"
     CDS             complement(<1..92)
                     /locus_tag="ACHK8E_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347340.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFST"
     gene            complement(184..260)
                     /locus_tag="ACHK8E_005245"
     tRNA            complement(184..260)
                     /locus_tag="ACHK8E_005245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Arg,seq:tct)
     gene            complement(274..350)
                     /locus_tag="ACHK8E_005246"
     tRNA            complement(274..350)
                     /locus_tag="ACHK8E_005246"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Arg,seq:tcg)
     gene            complement(358..433)
                     /locus_tag="ACHK8E_005247"
     tRNA            complement(358..433)
                     /locus_tag="ACHK8E_005247"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Ile,seq:cat)
     gene            complement(474..599)
                     /locus_tag="ACHK8E_005248"
                     /pseudo
     CDS             complement(474..599)
                     /locus_tag="ACHK8E_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(951..>1535)
                     /locus_tag="ACHK8E_005249"
     CDS             complement(951..>1535)
                     /locus_tag="ACHK8E_005249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0347341.1"
                     /translation="ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNA
                     DRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAV
                     VHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFME
                     TLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
BASE COUNT          437 a          356 c          322 g          420 t
ORIGIN      
        1 gtcgaaaagt ctatcgtaaa ttcccgggaa taggataccg aagaaaagcc cagtaacagg
       61 cacagtaccc attttaacaa tatacacttc atatacaggt attccttttg gctgaagtaa
      121 tcagcaccag acccggcgca gatataaaaa aggcccgcaa aagcgagcca gaaaaaataa
      181 gtatggcgcg ttgtactgga ttcgaaccag tgaccgattg cttagaaggc aattgctctg
      241 tccggctgag ctaacaacgc agtgtacaga taatggaccg ccatcgagga ctcgaacccc
      301 gcgcaaccag cttcgaaggc tggcgctctg tcccgatgag ctaatggcgg tatgtgatgg
      361 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
      421 gagctaaagg gccgggcgca ggataataac ggtacgtaac taatcctgca atatcatccg
      481 ttctgactgg ctaaatcctg aacttccctg accgtctgct caaaacgttc agtctccagc
      541 tcaacgccag ttgcacgacg ccccagcgcc attgctgctt tgacgctacg gacataaaaa
      601 agccagccac tgggggaggc tggcaaactc gtagagcaaa atgctgttac gcaaacttcg
      661 ttacagggtt atcctgcaat acttaaaata tacaatattt agaaaactaa tagtgccata
      721 tgcgattttt aagattttgt tattaattgc ggtcgcacct tcctttctgt gtactttccg
      781 tataacttac aggattctgg atacaaaaaa cccgcgcatc ggcgggttaa gcagcgtggc
      841 aatgtaacca cttttgtcat gatatgatga tttttacgat tgtaaactgt ttttctgcat
      901 cagggcacga taacctgacc attccccacc ctgtcaggtg gggatttgca ctaaacaacc
      961 ttctcagatg taatctgcct gtacgctacc agctttttat cagtgtactg gacaacatct
     1021 ttgatgacca gtttatgtac tgctagaatt agcgtctcca tgaaagaata atcagggtta
     1081 ttatcttttg acggcaaaga taccattaac tcgtcggcat cttttgcata gaaatttctc
     1141 ccgtagttga actttccggt acaagaagac ttatgaattg atgctgttat gaaaataacg
     1201 gcatttctgt caaagaattc tgtatgaaca actgccacat ggtcatctgc accatactca
     1261 taatttctgt attttgcgga gccaaacata tctatcgtaa tggtattttc agggaataca
     1321 acaacatcat tactgataaa tgcagaaata ccttcattgc cttcacccgc agtaacaaaa
     1381 ggcaaggagc cgggtatacg atctgcgttt ttcagacgct tacctcttat tgcggggcca
     1441 aataactttt tgaggttata agtgccccac ataagattat tgctatttaa tagctcaatc
     1501 gcgttatatt cctgtggtgt aagctcatag cttat
//
LOCUS       DAZGTJ010000255         1526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000255 DAZGTJ010000000
VERSION     DAZGTJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.261"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>1526)
                     /locus_tag="ACHK8E_005250"
     CDS             complement(<1..>1526)
                     /locus_tag="ACHK8E_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347342.1"
                     /translation="AQLTDRQKMDLAKERVADEVRAQLATDPELLAVNAMAAKLGDAQ
                     LFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAGMDVSPWEGVT
                     DAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAPQPEPVDPVAQ
                     HRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQAKRQAVMNELK
                     AKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQSQPENAEDGNGS
                     IYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGSDVFRNAAATG
                     LKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQPAFMRDPRFR
                     GFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQELTERPRLPAPGDIHPGQG
                     YPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPENAAGRQGETLEGEIVR
                     R"
BASE COUNT          270 a          496 c          378 g          382 t
ORIGIN      
        1 cgacgaacta tttcaccctc gagtgtttcc ccctggcgac cagcggcgtt ttccggtgcc
       61 ggagcacgtc ctttctggaa actctggccc ttaacctcac cggttgtggt aaaacgacca
      121 ccacgtccgg cctgattctc atccggcgta cgcgccacct ctcccggtaa cggatatccc
      181 tgtccgggat gaatatcgcc gggggcgggc agacgcggac gctcagttaa ctcctgtgct
      241 gtcggaccgg catcaccctc agccatctgt gaacgtacaa gctcctccgc catcggcgcg
      301 ttgccacggg caaggcgggc ctgtacctcc gtatcatccc ccgtgaaacc acggaatcgc
      361 gggtcacgca tgaacgcggg ctgctccatc gggtcagcat cagcaatacg ctctccgtca
      421 gccagggtgt taatggtttc cgttgccaca tcttcagaga acgcaggatt atccatttca
      481 acggcatact gattctcgcc tttcttcact acggacggtt tcaggccggt agcggcggca
      541 ttacggaaaa catcactccc catggcgctt ttctcatccg tgaaataacg gttgtccggg
      601 cgcacctttt caatgcgttc cgctggtctg gacgcaccat tcccgttaat ttcaatgcgg
      661 acttcattcg ggtcccggaa ccgcaccgtc gggtaaatgc tcccgttacc atcctccgca
      721 ttttcaggct gtgactgtgc ttccggcgtc acttcctgtt gtgggcgaat gacttctcgt
      781 attgcctgaa actgttgttc ttcagtctcg gtgcgctcct ctttctggct gagtctgcgg
      841 tattcctcaa gcagttcaga acgcggcttc gccttcagct cattcatcac agcctgtcgc
      901 ttcgcctgtt cgtccagttc attcaacaac tggctggcag cttcccggcg atgagctgcg
      961 gatgcgtcac cctctgttgc catatccgca tcagcatact gttccagaag ctgctcgcga
     1021 ttcatgccct gcatggattc acgatgctgt gccaccggat caaccggttc gggttgcggg
     1081 gctgcgtcgt cctgctgcac ggtttcagca tcacgcatgg ctgcttcttc tgcggcctga
     1141 cgtctgccac gatatccggc aaccgcaccg aatggagccc ccatcgcagc accgaaaaca
     1201 gtgccttcaa tccctgcgtc agtcaccccc tcccatggcg atacatccat tccggcggtc
     1261 tcacgcaatg ccatgttttc ctgatagcgc gaataggcgt tctgtgcccc gttaattacg
     1321 ccctgttccg tggcatttct gacaatgccg cttttaacgg tcttcgctgt gcctcgtgtc
     1381 accagattaa acagttgtgc gtcacccagt tttgccgcca tggcattcac agccagcaat
     1441 tcaggatcgg ttgccagctg cgcgcgcacc tcatcggcaa cacgctcttt tgccagatcc
     1501 attttctggc gatcagtaag ctgtgc
//
LOCUS       DAZGTJ010000256         1516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000256 DAZGTJ010000000
VERSION     DAZGTJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.262"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1516
                     /locus_tag="ACHK8E_005251"
     CDS             <1..>1516
                     /locus_tag="ACHK8E_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347343.1"
                     /translation="AQLTDRQKMDLAKERVADEVRAQLATAPQLLAVNAMAAKLGDAQ
                     LLNLAMRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTALRETAGMDVSPWEGVA
                     DATIEGAALGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAAPQPESVDPVAQ
                     HRESMQGMNREQLLEQYADADMATEGDASATHRREAASQLLNELDEQAKRQAVMNELK
                     AKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQSQPENAEDGNGS
                     IYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGSDVFRNAAATG
                     LKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQPAFMRDPGFR
                     GFTGDDTEVQARLARGNAPTTEELVRSQMAEGDAGPTAQELTERPRLPAPGDIHPGQG
                     YPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGRQGETLEGE"
BASE COUNT          375 a          381 c          496 g          264 t
ORIGIN      
        1 gcacagctta ctgatcgcca gaaaatggat ctggcaaaag agcgtgtggc tgatgaggtg
       61 cgtgcgcaac tggcaaccgc tcctcagttg ctggctgtga atgccatggc ggcaaaactg
      121 ggcgatgctc agttgcttaa tctggcgatg cggggcacag cgaagaccgt taaaagtggc
      181 attgtcagaa atgccactgc acagggggcg attaatgcgg cgcagggcgg ctattcacgc
      241 tatcaggaaa acacggcatt gcgtgagacc gccggaatgg atgtatcgcc atgggagggc
      301 gtggctgacg caacgatcga aggtgctgcc cttggtgctg cgatgggggc tccattcggt
      361 gcagttgccg gatatcgtgg cagacgtcag gccgcagaag aaaccgccat gcgtgatgct
      421 gaaaccgtgc agcaggacga cgcagccccg caaccagaat ctgttgatcc ggtggcgcag
      481 catcgtgaat ccatgcaggg gatgaatcgc gagcagcttc tggagcagta tgctgatgcg
      541 gatatggcga cagagggtga cgcatccgca actcatcgcc gggaagccgc cagccagctg
      601 ttgaatgaac tggacgaaca ggcgaagcga caggctgtga tgaatgagct gaaggcgaag
      661 ccgcgttctg aactgcttga ggaataccgc agactcagcc agaaagaggg gcgcaccgag
      721 actgaagaac aacagtttca ggcaatacga gaagtcattc gcccacaaca ggaagtgacg
      781 ccggaagcac agtcacagcc tgaaaatgcg gaggatggta acgggagcat ttacccgacg
      841 gtgcggttcc gggacccgaa tgaagtccgc attgaaatta acgggaatgg tgcgtccaga
      901 ccagcggaac gcattgaaaa ggtgcgcccg gacaaccgtt atttcacgga tgagaaaagc
      961 gccatgggga gtgatgtttt ccgtaatgcc gccgccaccg gcctgaaacc gtccgtagtg
     1021 aagaaaggcg agaatcagta tgccgttgaa atggataatc ctgcgttctc tgaagatgtg
     1081 gcaacggaaa ccattaacac cctggctgac ggagagcgta ttgctgatgc tgacccgatg
     1141 gagcagcccg cgttcatgcg tgacccggga ttccgtggtt tcacggggga tgatacggag
     1201 gtacaggccc gccttgcccg tggcaacgcg ccgacgacag aggagcttgt acgttcacag
     1261 atggctgaag gtgatgccgg tccgacagca caggagttaa ctgagcgtcc acgcctgccc
     1321 gctcccggcg atattcatcc cggacaggga tatccgttac cgggagaggt ggcgcgtacg
     1381 ccggatgaga atcaggccgg acgtggtggt cgttttacca caaccggtga ggttaagggc
     1441 cagagtttcc agaaaggaca agctccggca ccggaaaacg ccgctggtcg ccagggggaa
     1501 acgctcgagg gtgaaa
//
LOCUS       DAZGTJ010000257         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000257 DAZGTJ010000000
VERSION     DAZGTJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.263"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1510
                     /locus_tag="ACHK8E_005252"
     CDS             <1..>1510
                     /locus_tag="ACHK8E_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0347344.1"
                     /translation="VHYGYDDKGRLTGERQTVENPETGELLWQHETKQAYNEQGLTNR
                     VTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEYMRDRLHRETVRSFGSMAGNTAAYELT
                     STYTPAGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGYSATGRLASVRTTT
                     SDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGRLTEKTD
                     RIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRR
                     ERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTDTTRIQTVYEPGSFTPLIRIETDNGER
                     EKTQRRSLAEKLQQEGSEDGHGVVFPAELVRMLDRLEGEIRADRVSSESRQWLAQCGL
                     TVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISTEGATEWRGEYDEWGNLLNEENP
                     HHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYNYP"
BASE COUNT          358 a          425 c          512 g          215 t
ORIGIN      
        1 ccgtccacta tggctatgac gataagggcc gtctgaccgg ggagcgccag acggtggaga
       61 acccggagac gggggaactg ctgtggcagc atgagacgaa acaggcatac aacgaacagg
      121 ggctgacaaa ccgcgtcacg ccggacagcc tgccgccggt ggagtggctg acctatggca
      181 gcggttatct ggcgggcatg aagctgggcg ggacgccgct gctggagtac atgcgggaca
      241 gactgcaccg ggagacggtg cgcagcttcg gcagcatggc aggcaataca gccgcatatg
      301 aactgaccag cacttacacc cccgcaggcc agttacagag ccggcacctg aacagcctgg
      361 tgtttgattg tgactataac tggaatgaca acggcgacct gatgcgcatc agcggcccgc
      421 gacagacgcg ggaatacggc tacagtgcca cgggcaggct ggcgagcgtg cgcacgacca
      481 catcagacct ggatatccgc atcccgtatg ccaccgaccc ggcgggaaac cgcctgccgg
      541 acccggagct gcacccggac agtacactca cagtgtggcc ggataaccgc atcgcggagg
      601 atgcgcacta tgtctaccgc cacgatgaat acggcaggct gacggagaag acggaccgca
      661 tcccggcggg tgtgatacgg acggacgacg agcggaccca ccactaccac tacgacagcc
      721 agcaccgcct ggtgttccac acgcggatac agcatggcga gccactggtc gagagccgct
      781 acctctacga cccgctggga cggcgaatgg cgaaacgggt atggcggcgg gagcgtgacc
      841 tgacggggtg gatgtcgctg tcgcgtaaac cggaggtgac gtggtacggc tgggacggcg
      901 accacctgac gacggtgcag accgacacca cgcgtatcca gacggtatac gagccgggaa
      961 gcttcacacc gctcatccgc atcgaaacag acaacggcga gcgggagaaa acgcagcggc
     1021 ggagcctggc ggagaagctg cagcaggaag gaagcgagga cggccacggt gtggtgttcc
     1081 cggcagaact tgtgaggatg ctggacaggc tggaggggga aatccgggca gaccgcgtga
     1141 gcagtgaaag ccgccagtgg ctggcacagt gcgggctgac ggtggagcgg ctggcggcac
     1201 agatagagcc ggtttattta cctgagcgaa aaattcatct ttatcactgt gaccaccggg
     1261 ggctgccgct ggcgctcatc agtaccgaag gagcaacgga atggcgtggg gaatatgatg
     1321 aatggggcaa cctgctgaat gaggagaacc cgcatcacct gcaccagcca taccgtctgc
     1381 cggggcagca gcatgatgag gaatcaggac tgtactataa ccggaaccgg tactatgacc
     1441 cgctacaggg gaggtatatt acacaagacc ccattgggct ggagggggga tggaacctgt
     1501 ataattaccc
//
LOCUS       DAZGTJ010000258         1503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000258 DAZGTJ010000000
VERSION     DAZGTJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.264"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..113
                     /locus_tag="ACHK8E_005253"
     CDS             <1..113
                     /locus_tag="ACHK8E_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HFP0347345.1"
                     /translation="IAELNAELKTTVSKIDQLRKDIDAIVAEIEGCGVQK"
     gene            110..1313
                     /locus_tag="ACHK8E_005254"
                     /pseudo
     CDS             110..1313
                     /locus_tag="ACHK8E_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          469 a          306 c          326 g          402 t
ORIGIN      
        1 atattgctga gctgaatgct gagctgaaaa ccacggtcag caaaatcgac cagttgcgta
       61 aagatattga tgcgattgtg gctgaaattg aaggctgcgg ggtgcagaaa tgagcgagtt
      121 gagttatctg gaaaaattgc tggatggggt tgaggttgag tggttgccat tgggggaagt
      181 ctcagcctta agaagaggca gagttatgtc aaagggatat ttaactgaaa attttggccc
      241 atatcctgtc tatagctctc aaactgcaaa taatgggaaa ataggttcta tcaatacctt
      301 cgattttgat ggcgaatata taagctggac aacagatggc gcaaatgcag gcactgtgtt
      361 ctatcgaaca ggtaaatttt caataactaa tgtttgcggc ctaattacat taaaaagtaa
      421 atatccatta atctacaaat ttctgtttta ctggctaaca atagaggcca aaaaacatgt
      481 ttactcagga atgggaatcc caagttaatg agtcatcagg ttgaaaatat acctgtacca
      541 atcccctgcc cggataatcc ggaaaaatcc cttgccatcc agtctgaaat cgtgcgaatt
      601 ctggacaaat tcagcgccct gacagcagag ctgacagcag agctgacagc agagcttaac
      661 atgcgtaaaa aacaatacaa ctactatcga gataagttgt tgaatttcaa agatattgcg
      721 ggggggggta attacatcct tccggacgcc atggatggta ttcggattga gtggaagact
      781 ttgggggaag tggctgttat aggaacaggt aaccatgata cgcaagatgc tatagaacat
      841 ggcaaatata ttttttatgc acgtggacgt gaaccattaa agttaaatgt atttgatttt
      901 gatgaaacag caatcattac ggctggagat ggtgccgggg tggggaaggt atttcattac
      961 gctaaaggta aatatgcttt gcatcagaga gcatatcgta ttgtcccaaa tgcatttatg
     1021 aatccgcgat ttgtatatca ctatataaca gcatattttt tacctatatt caaaaagcat
     1081 cagtaagttc atcggttacc tcacttcgtc gaccaatgtt tttaaaattc ccaatacctg
     1141 taccaccttc agaagaacaa gctcgcattg tcgaaatact cgataaattc gacaccctga
     1201 ccaactccat caccgaaggt ctcccgcgtg aaatcgagtt gcgccagaaa caatacgagt
     1261 actatcgtga tttactgttc agtttcccga aacctgaaac cgtcagtaac taattgacaa
     1321 ttgctaccga ccgggccacc ttaacatccg gtcagtatat aaaccatttt tacacgccgg
     1381 aagtcaccct taactccctt ccggccccgc cagacggcac aaaggatgcg ctatgattct
     1441 tcagacacac accattgctg aaaccagtaa ctttatcgtt cttgataagt acatcaaggc
     1501 tga
//
LOCUS       DAZGTJ010000259         1480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000259 DAZGTJ010000000
VERSION     DAZGTJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.265"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(758..1000)
                     /locus_tag="ACHK8E_005255"
     CDS             complement(758..1000)
                     /locus_tag="ACHK8E_005255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347346.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            1240..>1480
                     /locus_tag="ACHK8E_005256"
     CDS             1240..>1480
                     /locus_tag="ACHK8E_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0347347.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGQYSQANAEKSISLGASARAIENNSTALG"
BASE COUNT          461 a          317 c          275 g          427 t
ORIGIN      
        1 tgttgcccgt aaacatttaa gttcgcaggc taacattatt gccacaagcc ccgtatttcc
       61 ctggtgaatc ctcaaagcga gtccctcacc atccagacaa aacacagtat cgtcgattaa
      121 taatcactaa aagcgttcgt cgtagcatca taccccctga atgaacacat ccctgtttgc
      181 aaggattctg ttgttctgat atcgacgagt agctgcaact ctgtatccgc acttattgca
      241 atcagtgcag tctggccacg atgcgattcg ctgacctgag ccttattctc caggcggact
      301 ttatgcaatg ctcccctgaa atcttccatt attttacatg tacaggcatc atattaaata
      361 tggaaataat aacgaacgga aaagggacac aatgaatatc aaagcaccag tataaaccag
      421 gatgttttga gcaatttctt actctcctga atacacactg taaatcatta gaaactgcac
      481 tagccatgcc aagtacactc tgctacgaac tgatgaggaa gtgcggtatc aattacctgg
      541 acagcctgat caacggagag gtatatctat tatctatgta attacagggg gactgatatc
      601 agcgaaaaag caggtcagca tattcactgc ccctggtctg aactgaaaca acgtcagcca
      661 gaaataatcc tataatgcct catataagtg gataactttt catccgtcgt tgacatcata
      721 cacataaaac attgataaca gtcatgaata caccgaatta tctttctgac tcagcgtttc
      781 cagtcagtag catttcttcc ggtaaaggca tttccgaaac gtcacgttcg ccattaaata
      841 caaaaatact ttctgcgtaa tcattctgta acaaagaatt agttaacggc agattttttg
      901 tttgcatacc ggtggcccac tgaaaaatcc cggggaaacg acgggcactg atgtgtacag
      961 aattgaggtg gtacgcagta atatctcttg ccatcatcat aagatggcac atcatatccc
     1021 gaacgcaaac ctggcacatg atgaacagac ggaaggctca gcaggacttc tggataatta
     1081 tacacgatag ccagttactg ctgacctgga tttattatca taccacatta tttatttcaa
     1141 aaaataaatt ttgaattact tgtttttact tgtatataaa tatccaacga tggttctgtt
     1201 tgtattatgt atactatata aaagagatgg tgaattcaaa tgcgtaaaat cagtgtcagt
     1261 tatatatgca aatcattatt tctctactgc tcagcttctt tacctgtctt atctgcaaat
     1321 ggtattcctg ataaaatgat cgtggaactt ggtgataaca gtgcggcaaa agctaatttc
     1381 gctgttgctg ttggtcaata ttcacaggca aatgcagaaa aaagtatttc tttaggtgca
     1441 tcagcaagag ctattgaaaa taactctacc gcgttaggtg
//
LOCUS       DAZGTJ010000260         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000260 DAZGTJ010000000
VERSION     DAZGTJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.266"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHK8E_005257"
     CDS             complement(<1..1001)
                     /locus_tag="ACHK8E_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0347348.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(998..>1453)
                     /locus_tag="ACHK8E_005258"
     CDS             complement(998..>1453)
                     /locus_tag="ACHK8E_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0347349.1"
                     /translation="SDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          321 a          425 c          385 g          322 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccactgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgt cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgcccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgttc aagacggctg
      421 tccatttcat agcgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagttcc ctaggtggat actcccggga gagccggaat
      541 tcaaaatcct gcccttccag accatgttta cgtaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcgat acggggaaca
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcaaaga tacgacgaaa cgactcaacc
      721 acgccgaata ccgtcctgat cggcagggcc ggtgttccgt cagacatcgg gacctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaagctgc
      961 tgtacaacgc tgccagcctt gctggtcagg gaacggttca tgacggctct ccctctgcct
     1021 gtgcatcaaa taccatcaca ataccgtcct cgtcactcca tgtcagagtc agcgcctgtg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cagacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtctggaaa cgggcaagca gtgctggctg gaagtggtcg cgcagcagtg
     1381 gacggataag ctcatgcagc tccgattccg ttgtctccgg cttttcgctc agttgctgca
     1441 tcagaaggtc act
//
LOCUS       DAZGTJ010000261         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000261 DAZGTJ010000000
VERSION     DAZGTJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.267"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHK8E_005259"
     CDS             complement(<1..1001)
                     /locus_tag="ACHK8E_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0347350.1"
                     /translation="MRSFLTSKAGSVVQQFSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYLVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(986..>1453)
                     /locus_tag="ACHK8E_005260"
     CDS             complement(986..>1453)
                     /locus_tag="ACHK8E_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0347351.1"
                     /translation="SDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLARFRTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          322 a          432 c          391 g          308 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccgctgaca
       61 agatactgcg cattcagaat acgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cggggtggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga ggagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaaactgc
      961 tgtacaacgc tgccagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagtaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagctgct
     1141 ggttcagcag actctccaca ttccgggcac ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cacacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtccggaaa cgggcgagca gtgcaggctg gaagtggtcg cgcagtatcg
     1381 ggcgcagcag ttcgtgcagg ctggcctcag tggcttccgg ttgttcgtcc agcaactgca
     1441 tcagaaggtc act
//
LOCUS       DAZGTJ010000262         1442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000262 DAZGTJ010000000
VERSION     DAZGTJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.268"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..546
                     /locus_tag="ACHK8E_005261"
     CDS             <1..546
                     /locus_tag="ACHK8E_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP0347352.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLSSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGANLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..>1442
                     /locus_tag="ACHK8E_005262"
     CDS             562..>1442
                     /locus_tag="ACHK8E_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347353.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPLPFKGEQAIRCMADGEVIAYRICRDYLT
                     LGWESGPLSFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWDPADSNLNATGSNKRKYGLVTL
                     KGLPEKTSSTLTPGQRYWVVVDNNNVKPAAGAGPGWWRHLLPPAKEAMVFDKTVSLRS
                     PFAIKAGDPIGHMGYYQA"
BASE COUNT          383 a          337 c          396 g          326 t
ORIGIN      
        1 attaggtttg taaccgataa aatcagggaa cttcagtctg cggtcctgct gggtagcgcg
       61 ccacaggggg tggccctgac ctcaggtgaa caccttcagc tcagcagtac ccgtaatacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caatcaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tttcagaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaaccctgac gctgaacggt
      361 ggcgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg caaatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt gggaggagaa
      661 tctaccgggc tgtacatgat aggccgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccgcttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgcta ggctgggaat caggtccgct aagtttttcg
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagcggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtggg acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaccagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cggcgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctccgttccc cattcgccat taaggctggt gacccgattg ggcacatggg ctactatcag
     1441 gc
//
LOCUS       DAZGTJ010000263         1431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000263 DAZGTJ010000000
VERSION     DAZGTJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.269"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1137)
                     /locus_tag="ACHK8E_005263"
     CDS             complement(<1..1137)
                     /locus_tag="ACHK8E_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFP0347354.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGM
                     AQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWG"
     gene            complement(1130..>1431)
                     /locus_tag="ACHK8E_005264"
     CDS             complement(1130..>1431)
                     /locus_tag="ACHK8E_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347355.1"
                     /translation="NELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLG
                     YTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT          332 a          420 c          376 g          303 t
ORIGIN      
        1 cccccacagg acaatcaggg aaagcagaat caatcgcagc atcttcagcc agttaaagcc
       61 cagcttcctg ccgctgacgg catcacagtc atcaataata gctttccagg caggtgtgtc
      121 ggggcatgtg tttggtaccg ctccggcaac ggtcagttca ggactgaaca tgacaccgcg
      181 taaacgccag gcagcagtcc cctgcatcag tccactgagc aaaacagcca ggctggcttt
      241 cagctcctgc cgcagtctga gtgacaactg aaggagaccg aaatcagcag gttcattcag
      301 tatctgcgcc attcctgctt tttgcaggtg taacgccagt gtggaaaacg cttcgtccgc
      361 accgttcacg gtaccttccg gtccgaagat gacccctgtt gcggccgctt tcgcgtcctg
      421 ccgggcaccg gtagcgttgt cagtcagcca cagccagact ggtgcctgcc agcccaactg
      481 atggtcggct ttctgacggt aacgcaggag ggcatcccgt tcgccatccg tcaggagggc
      541 tgaaatactc atcacctgaa tgatgccatc aaccggttga ccggatcgca gacgcttcag
      601 ttttgaaagg aagatctcgt ccgggagtga ctgcgcgtct ccgccataaa tcagcacgta
      661 gccgtcgcct tcctgccaga ggtcatggca aaggcccggg gctgctttcc ggaccacatc
      721 cggatccccc atcaccagca ggagtcgcac cttacgctgc cagcgacgcc catagcgaaa
      781 gcgcaaatgt tctgaaacag catcttcgct aaaaaccggc tcatcgtcgt cttcttcacc
      841 gggaatggtg ttcgtcaccg gagtgatact ctcagccttt ttctgccccc actggaaata
      901 cagcccggat ttaccggcga agtcgaaagc cttctccgtc agccagccga gtaaaaacag
      961 cgtggcaacc agtatggcgc agaatgtgag tatgcgcagc ccgtcatacg gaccgaagcc
     1021 ggtggccgaa gcgacgtttt ccggaaaggc ataaaaaaga aatgccacga tgaccgtcag
     1081 aaaaaaggcg acagtcacaa cagagccgat gagtgtttta acttttacct tagccattcc
     1141 cgggtgattc cttgcgaact tcttctttag tgatgacggc aacccagacg tcgtctttgt
     1201 cagctgtggg ctgggcggcg ataacctgcg cacctcgttg ttcaaaggca gcatcagcaa
     1261 gcgtgatggc aagccatgga gcagcatggc cggtatagcc cagcacggga tcgatactga
     1321 aattgtcttc tgaaaggctg aatgtcactc cgttctcttc gcaggcatta ttccagctgc
     1381 tgcccgacgt caatgtggga ccggagatcc agcttccttt aagctcattg g
//
LOCUS       DAZGTJ010000264         1429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000264 DAZGTJ010000000
VERSION     DAZGTJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.270"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..229)
                     /locus_tag="ACHK8E_005265"
     CDS             complement(<1..229)
                     /locus_tag="ACHK8E_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP0347356.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(325..801)
                     /locus_tag="ACHK8E_005266"
     CDS             complement(325..801)
                     /locus_tag="ACHK8E_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP0347357.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            complement(864..1070)
                     /locus_tag="ACHK8E_005267"
     CDS             complement(864..1070)
                     /locus_tag="ACHK8E_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347358.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(1198..1429)
                     /locus_tag="ACHK8E_005268"
                     /pseudo
     CDS             complement(1198..1429)
                     /locus_tag="ACHK8E_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
BASE COUNT          392 a          428 c          281 g          328 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt cgtatcactt atattattct
      121 tatacagaaa accgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcatt ccctccagaa
      241 aataactgtt atcacaaata aaatgcatac ccgttccaca tgaaatttat cacgcctggc
      301 cacataacat taattatccc ggatttaccg gtctgcggtg ctgcagagat atttaatact
      361 gacgccgcgc agaccatttt tctccatcgc attgtaaaga tgcgcacata ccgtcttcat
      421 gcttatcccc agctggtctg ccagaaaggt aaaaccgtac tcatggaaaa caactccgaa
      481 gaccaccacc atctcggtac gggtcagtgg tcttgccgta tgtccgaaca gccacgcccc
      541 acgacggatg gcctgcctga tatctgtcag gcatctcaca gaggaaacag gaatgacaag
      601 aaaacgacgc ccacagaaca tacagtatgt aagcctttcc agattacggt cacagagaat
      661 cacataactg tcttcgggaa gtggcagacg gcgctttccg gcatcaaaca gttcacggcc
      721 tgtcgtgagc ctgtatccgt tcagcatacg catcaggcca tattccagat atctgtcacg
      781 ggtcataata ataatctgca tactctcaga cccttatctc ccggataccg gctccgttca
      841 ccattaccgg tataactgac tcatcattca taacggaaac tgtctgccgc atacatcagt
      901 gcccgctctt ccagctctga cagactcagc tctttcagtt ccccttctcc ttccgcaaga
      961 agacaacatt caaaaacagc tgcatcgtcg cattccagac gaacctgcac cctgtcagcc
     1021 cgaccgttta cagtaaatac ctgcactgaa cttatgcgat agttcagcat atacactctc
     1081 cctctcagct cctcaggaat accccggaac cgtcagtcat gccaccgggg agaaaagcag
     1141 gcagcgaatc atgaccgcat acagaacgct gcccgccccg gtgactcaga cccgggttca
     1201 gaactcccag ttgacaccca cattccagga aacatcaccg tcactgaacg cagtacctgc
     1261 ctttgccgca gcctgctcgt taaagcgata tcccatgccc accgcaacag cctgcctgtc
     1321 actgtccgcc cacagccgct gtggcgttaa atttacccac actgtatggc tggaaaagac
     1381 cggccagcgc agctgactgg gctgctgccc gcttcatttc ctcatgatt
//
LOCUS       DAZGTJ010000265         1394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000265 DAZGTJ010000000
VERSION     DAZGTJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.271"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1394
                     /locus_tag="ACHK8E_005269"
     CDS             <1..>1394
                     /locus_tag="ACHK8E_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFP0347359.1"
                     /translation="QNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQQAE
                     VRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGEEIKGAAKHRY
                     AQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPAKTK
                     HNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPPSQMD
                     RASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAKVWFAE
                     QEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDARKY
                     IDSHRDELVRHVKDMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFG
                     NYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPG
                     EVAINLTKGNGPGAL"
BASE COUNT          376 a          330 c          413 g          275 t
ORIGIN      
        1 cagaacgcga cagcaccggt acgtgaaggg ctaccggctc ctgacattgc gcgtaatgtt
       61 cgtatgcctc agcctgaatc acttccccgc actgtacggg actcactgcc tgagctcgca
      121 cagcaggcag aagtacgccg acaggccgga ggaaatcgtg acatcccgca gcctgagaca
      181 atcgcacctg aatctgaaac aactgtcttt actgacaggg aatctaccgt gcgcggaggt
      241 gaagtcaggg gcaaaaaaat tgaagacttt ggcgaggaaa ttaaaggggc agccaaacac
      301 cgttatgcac agcttgctga aacactgggt aaaacgctgg aagacaggga ttatgccacg
      361 cagccgctga gcaaactgtt cccgaaaccg gactacgcaa aactggcgaa cgaaggtgcc
      421 gatgctgaca ctctggcaat gatagcgctg tatcgtagcg atattccggc gaagacgaaa
      481 cacaatacgg caggctgggg ggagagcgta aaaaaagtac gacacagtgt atcggaaatg
      541 ctgaacggaa cggtcagcgc gaaacgcctc gcagaatgga tggaaggcag aatgccctcc
      601 cgttacgcgg atacctggca actgttacgc actctgccac cctcacagat ggacagggct
      661 tctgcttatc gggtggtatc gggtgtgtat caggcggcag gagggaagcg ttacgatccg
      721 ccacagaaac tttattcact gcgcaataag gacaataagg ggagtaacct ctttttctcg
      781 gaaagcaggg atgaattact ggcaaaggcg aaagtctggt ttgcagagca ggaggaaaaa
      841 tcacaggcga aaggtgatga aaaaacagca ccgtcaccgg atgacaaaat ccgctttgac
      901 gtttaccgga atacccgcag tggcgatatt tttatcgctt acggtaaaaa caaaatgcgg
      961 gtgagaggtg gctttaagtc agccagtgat gcgcgtaagt acattgattc acatcgtgat
     1021 gagcttgttc gtcatgtgaa ggatatgcgg gagatttcgc gtgaggagca gcgtaacgcc
     1081 accaaccgcg accgtaccgg accagaacgc cgtaaagggg atgtttcacc ggagcagttc
     1141 agtgatgcgt ttggtttccg tggtgtgcag tttggtaact acgtggaagg tccgcgtcgt
     1201 caggctgatt tgaaccgggc ttatgactcg ctgcatgacc ttgcggaagt actgaatgta
     1261 ccgacaaaag cgctttccct gaacggtcgt cttggcctgg catttggtgc ccgtggtaag
     1321 ggtaaggcgg cggcacacta tgagccgggt gaggtggcaa tcaacctgac aaaaggtaac
     1381 ggaccgggtg cgct
//
LOCUS       DAZGTJ010000266         1392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000266 DAZGTJ010000000
VERSION     DAZGTJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.272"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            22..540
                     /locus_tag="ACHK8E_005270"
     CDS             22..540
                     /locus_tag="ACHK8E_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0347360.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESRLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            537..1130
                     /locus_tag="ACHK8E_005271"
     CDS             537..1130
                     /locus_tag="ACHK8E_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0347361.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            1127..>1392
                     /locus_tag="ACHK8E_005272"
     CDS             1127..>1392
                     /locus_tag="ACHK8E_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347362.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAIS"
BASE COUNT          292 a          336 c          436 g          328 t
ORIGIN      
        1 tgaaccacag gaggagcaga aatgaaacga gaaagacagt tttatgtact gagtctgctg
       61 ctgttctgta caacagctgg cgcagaactg aaagtgattg cagacctggg cggcagggat
      121 gccagcccct tttttgaggc gataaaccgg cagccaccgg ttcccgcttc agttgtgcca
      181 tcaccctctt ttccgcaggg agaggcggca atgttaccgg tcagtacgcc tgaaatgtca
      241 cccggagagg ttgtggcgcg ccggcttcag ttaccgggca tcggtgcact ttttcttgtg
      301 ggcgatgatg cccattcccg cacatggctg cagatgaatg cagcagtact cagggagcgg
      361 aaggcggcgg gaatggtagt gaatgtggcg gatgagagcc gtctgcgcgt actgcgtgag
      421 ctggcaccgg gggtggtgat ggttccggca tccggttctg tactggcccg caggttacag
      481 ataagtcatt acccggtgct gataacggac aacggcctgt cacaggtacc tgcaccatga
      541 tgccgctgtc tgccacttct gctgcgctct ctgcttctcc ggtactgaca gcctgcctgg
      601 ttctcagcct gctggctgtt gtcctggggc cggcactgtg gcgcagccgg cgaaaaaaag
      661 cgtgtgtgag aacacgccgg catcagcgtt atcgtgccac ggcctcacgg gtaatgaagc
      721 ggctgaacga attgcagggt gatgcccggc ggctgaaata tctgcgcaaa atcagtccgt
      781 atgtgtttga ggaactgttg ctgctggctt ttgagcaaca ggggatgccg gttatccgta
      841 acccctcata cagcggcgat ggtggtcttg atggtcaggt ggtgattaac ggtgaacgct
      901 ggctgataca ggctaaacgt tacggaagaa ccattcgtcc ggcacatatt gatgcttttg
      961 gcgaactgct gcgccggaag ggatgcaggg gcttttttat ccatacgggg cgtacgggga
     1021 acatcagccg ggcacggctg acactgtggc cggatattga gctgatcagt ggacaacgtc
     1081 tgttgcaact gctgaatggc accttactga gtaataaagg aagatcatga ttatgacact
     1141 gtaccggttt gttatgctgc tgctccggct tctgattgtc tggccgtcaa tgctggcagg
     1201 atgtctggta atgctgtatg tctggaacaa tctgagtggt ggtgaggcag gcatgacaca
     1261 gcgttatgcg acggagtcat cccgctggcg tgatgcgcct gaagggcatg tgatggtaca
     1321 aaaatgttcc tccgtcatat ccgtctcagg tatgtatgtc gatgagcagg aatgtccgcg
     1381 aacagcgatt tc
//
LOCUS       DAZGTJ010000267         1375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000267 DAZGTJ010000000
VERSION     DAZGTJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.273"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            118..1329
                     /gene="bluF"
                     /locus_tag="ACHK8E_005273"
     CDS             118..1329
                     /gene="bluF"
                     /locus_tag="ACHK8E_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP0347363.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          390 a          289 c          295 g          401 t
ORIGIN      
        1 tttttccatt ttttatcaat gtaaagaaac agttatgttt tggtattaaa agaaagacac
       61 cggaagatat gtctgttacc gtcttactct cgcctctccc attaccctgg acagttaatg
      121 cttaccaccc ttatttatcg tagccatata cgtgacgacg aacctgtcaa aaaaatcgaa
      181 gaaatggttt cgatagcaaa tcgcaggaac atgcagtctg acgtaacagg gatcttactg
      241 tttaatggtt ctcatttttt ccagcttctg gaaggtccgg aagaacaggt taaaatgata
      301 tatcgggcta tatgccagga tccacggcac tataatattg ttgagctgat gtgcgattac
      361 gcgcctgctc gccgttttgg caaagcggga atggaattat ttgatttgcg cctgcacgag
      421 cgagatgacg ttttacaggc cgtattcgac aaaggcacat caaaatttca gctaacttat
      481 gatgacagag cgctacaatt ttttcgtact tttgtccttg caaccgaaca agcaacctat
      541 ttcgagatcc ctgccgaaga ctcctggctt tttatcgctg acggatctga taaagaactt
      601 gattcctgta ccctttcacc aaatataaac gaccactttg cctttcatcc tattgtcgat
      661 cccttatcgc ggcggataat cgcttttgaa gccattgtgc aaaaaaatga agacagcccg
      721 tcagctatag cggttgggca gcgtaaagaa ggggaaatct atacagcgga tctcaaaagt
      781 aaggcgcttg cattcgcgat ggcacacgca cttgacctcg gtgataaaat gatttcaatc
      841 aatctattac ctatgaccct ggttaacgaa cctgacgcag tctctttttt acttgatgaa
      901 ataaaggcca atgctctggt gcctgaacaa atcatcgttg aatttactga aagtgaagtc
      961 atatctcggt ttgatgagtt tgccgaagcg gttaaatcgc taaaggctgc tggtatcagt
     1021 gtagcaattg atcattttgg cgcaggtttt gccggtttgt tactcctgtc acgcttccag
     1081 cctgacagaa ttaaaatcag tcaggaattg attaccaatg tccataaaag tgggccacgg
     1141 caggcaatta ttcaggcgat cataaaatgc tgtacatcac ttgaaattca agtctctgct
     1201 atgggtgtga caacaccaga agagtggatg tggcttgaat ctgcaggaat tgagatgttt
     1261 cagggagatc tgtttgcgaa agctaaattg aatggtatcc cttcagttgc gtggccagag
     1321 aaaaaataat tttcagcaca ttatttcaca tgatttcaca tgatttccgt aaatc
//
LOCUS       DAZGTJ010000268         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000268 DAZGTJ010000000
VERSION     DAZGTJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.274"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..388
                     /locus_tag="ACHK8E_005274"
     CDS             <1..388
                     /locus_tag="ACHK8E_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0347364.1"
                     /translation="NKYGIPSNPFSLKHHNCLYASSLVGGNCWHFYIRDEITTLEIAK
                     TVNIENSEFLKAVALMGGGIAYLPYPLVQSEILSGKLVSIMNDYIKTRFEVSLWFRKP
                     LSLRCVTFRNFLVERASQLKDTVQFR"
     gene            562..1369
                     /locus_tag="ACHK8E_005275"
                     /pseudo
     CDS             562..1369
                     /locus_tag="ACHK8E_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          413 a          293 c          323 g          340 t
ORIGIN      
        1 aaataaatat ggaataccct ccaatccatt cagtttaaag catcataact gtttatacgc
       61 atcttctctt gttggaggga attgttggca tttctatatt cgtgatgaaa taacaacatt
      121 ggaaatagct aaaacagtaa acattgaaaa tagtgagttt ttaaaagcag ttgcactaat
      181 gggtgggggg atagcatact taccatatcc tcttgttcaa tcagaaatac tgtcaggaaa
      241 actagtatca ataatgaatg attatattaa aactcgtttt gaagtaagtc tgtggtttag
      301 gaagccccta tccttgcgtt gtgttacatt taggaatttc ctagtagaaa gagcctcaca
      361 actcaaggat actgtacaat tcagataaat cacttaactt gatgcatcaa tcacccagcg
      421 accagttatg ctcatcataa tcattattca ggtgtgagca taatgagcat aacccagcga
      481 tccagacagg ttccctgaat ctccagacaa ccaatatcaa ttaaataagt gatagtctaa
      541 aaactagtca ttagagaaca gatgattgat gtcttagggc cggagaaacg cggacggcgt
      601 accacacagg aaaagatcgc tattgttcag cagaactttg aaccgggaat aactgtctcc
      661 ctcgttgcac ggcaacacgg tgtgacagcc agttatttct ctggcgtaag caataccagg
      721 aaggaaatct cactgctgtg gctgctggag aacaggttgt tctggcctct gagcttgctg
      781 ccgccatgaa gcacattaaa gaactccagc gcctgcccgg caagaaaacg atggaaaatt
      841 aactcattag agaagccgtt gaatattggc gtgcaaaaaa gtggatagcg cacgcccaat
      901 attgcccggg aatggggagt aagtttcgtt agccgttatc tccgtgtgtt gcgtgcgcag
      961 ttgcacgtca ttctcagacg aaccgatgac tggaaggatg gccgccgcat gatacggatg
     1021 tttatctccg taataacatg gtgtacacca tgttatcgga gagctgccca cgtatggtta
     1081 tcgtcggctg tgaaacagta caggacgtca tgctgggagc ggtagaacac cgcttcggca
     1141 gcgaccttcc tgcgtctcca gtggagtgcc tgacggataa tagttcatgc tatcaggcta
     1201 atgaaacacg ccagttcgcc cggatgttgg aacttgaacc gaagaacacg gcggtgcggt
     1261 atccggagag taacggcata gcagagagct tcgtgaaaac gataaagcgt gactacatca
     1321 gtatcatgcc caaaccagac gggttaacgg cagcaaagaa ccttgcaga
//
LOCUS       DAZGTJ010000269         1356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000269 DAZGTJ010000000
VERSION     DAZGTJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.275"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..202)
                     /locus_tag="ACHK8E_005276"
     CDS             complement(<1..202)
                     /locus_tag="ACHK8E_005276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347365.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGE"
     gene            315..881
                     /locus_tag="ACHK8E_005277"
     CDS             315..881
                     /locus_tag="ACHK8E_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347366.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            complement(1265..>1356)
                     /locus_tag="ACHK8E_005278"
     CDS             complement(1265..>1356)
                     /locus_tag="ACHK8E_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0347367.1"
                     /translation="VYPNLLIWPAAMLLGVAITVTMASFKKYL"
BASE COUNT          379 a          333 c          299 g          345 t
ORIGIN      
        1 gttccccctg tgaagaagcc tgcagagagg ccgttctggc ccgctccagc gctgcctgca
       61 cgtggtcaag ctggcgcatt acagtggcca gttcatcctt ttcggcagcc tgcgcaggta
      121 atgcaaagga caaaagaaac agcatccccg cggcaccggc cagacggccg gcacggcagg
      181 atgatgccgg aaaagaaaac atacgcacct ccgtcagagt taaacagtgc gtacaggatg
      241 cgtaaggcag gagactggat cagcgataaa ctctatttgc aaaacagaaa atttttacgg
      301 gagaatacac tggtgtggat gaaggcaaac aacccggaac tcctggcatg aacgacatca
      361 tcagtataat cattctcctg acagtctatc tcatcggcct gatattcatt atcctgacta
      421 tcaatgatat agacagatca tcaaaaaaga gaaagtacga gaaaaaactg aaagaagcga
      481 taataaatgg cagtttacaa aataatgata tctgtttact ggccgaacgc tggtctgcca
      541 gtcatgaaca tatcaaaagc gcactgctgt ctgttttcaa tgattttctg actgataaca
      601 acttttcaca tcacgtcccc cgggtcagag aactgatcac atggtacgag aaatatgccc
      661 cattcacaga actccctgag gatataaaac aacagttaca aaatataatc agtttacccg
      721 atgtcagtga gtcagaagtg cagttattag ccacgtcact tcatgacatt tataattcct
      781 gtcagaaaaa agagaagctg gccagaagaa taaactatgt tctgggggct gtcagcatag
      841 ccagtttctg catctcgctg ttccagattc tgttcaaata aatttattcc agcctgaatg
      901 atttattcct gtttattaaa acacggcatc actactgttt cccgttaatg gcaccgcatt
      961 aacaatttaa agaggaaaca catcagcatg gaatttctcc gtactgccct tccgttctgg
     1021 gccctgttcc ttctgacctg ggtttgtgtt gtgtattttt tcatttatga agaacctcgt
     1081 tgccccccgt ggatctatga cgtcatcagg gccatcactc tgttcattat tttcgtcaac
     1141 attgttgtca ttccggcgct gtcgttactc tgacactgac gcgcactgac cgtaagggcg
     1201 tggcttgtct gccgacaccc tgccacaaaa caagttttgc atcagggtgt ccggcctctg
     1261 tggattataa atatttcttg aaggatgcca tggtcacggt gatggccacc cccagcagca
     1321 tggccgccgg ccatatcagc aggttcggat acaccg
//
LOCUS       DAZGTJ010000270         1350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000270 DAZGTJ010000000
VERSION     DAZGTJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.276"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1204
                     /locus_tag="ACHK8E_005279"
     CDS             <1..1204
                     /locus_tag="ACHK8E_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0347368.1"
                     /translation="SVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIV
                     SVGSDTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQV
                     ITETRARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQA
                     RTAGDTETLAAARTHSDENDKRTLVSANTYADQKETAINNRTDGLLMTERQARIAGDT
                     ATLAAARTHSDENNKRTLVSANTYADQKETDINNRTDGLLMTERQARIAGDAETLATA
                     AAYSDAGDSLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAG
                     VTAISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAI
                     GAGVAGGW"
BASE COUNT          426 a          358 c          325 g          241 t
ORIGIN      
        1 cagcgtcagc ctcggtaatg ccaccagaat taacggtgat tcatctgttg ccataggtct
       61 gacatcgtcc gtaaccggga acgactccgt ggcactgggc agtcattcca gtgctaacgg
      121 aaacaatatc gtatccgtcg gctctgacac actgaaacgc cgcatcacaa atctggcaca
      181 ggggaaagat gaccatgatg cggtaaatct gatgcagatg accagtgctg ttaaccggga
      241 agctggagaa agaataaaat caacaaacaa cctgcagaat cagcttaata ccctctctgg
      301 tcaggtcata actgaaacca gggcccgaac agaaggtgat gcagcagcgc tggctgcagc
      361 cagagcccac agcgatgaaa acgacaaaag aacactggtc agtgcgaata cctatgctga
      421 ccagaaagaa accgccatca ataaccgtac tgacggatta ctgacgaccg aacgccaggc
      481 acgcaccgcg ggcgatactg aaactctggc tgctgccaga acgcacagcg atgaaaacga
      541 caaaagaaca ctggtcagtg cgaatactta tgctgaccag aaagaaaccg ccatcaataa
      601 ccgtactgac ggattactga tgaccgaacg ccaggcacgc atcgcgggcg atactgcaac
      661 tctggctgct gccagaacgc acagcgatga aaacaacaaa agaacactgg tcagtgcgaa
      721 tacctatgct gaccagaaag aaaccgacat caataaccgt actgacggat tactgatgac
      781 cgaacgccag gcacgcatcg caggtgatgc agaaactctg gcaacagcag cggcatacag
      841 tgacgccggg gattcactga cactgaaaca ggcaacagcc tacagcgata tcagtacccg
      901 ccgggcactg tcagaggcca atcgttatac cgacaacaaa ttcagaaacc tgagcaataa
      961 aattgaacgg gcagaaaaaa gacttaatgc aggcatcgcc ggagtgacag caatatcctc
     1021 catcccctat gtggctgaaa attctttttc atacgggatc ggcgtgggta actatcagaa
     1081 cgctaatgct gtggccgcag gcattcagta taaaacatca cgtaatacca atatacgcct
     1141 caacatctcc tgggactcat ctcataacac cgcgattggt gcaggggttg ccggcggctg
     1201 gtaaataatc ttgcgtacat acggcattat ggtattccgt aatcacatta attcagtacg
     1261 caacacagcc ccatccggaa cagaccactg gactgagcgg gataatcaag gaaaaagggc
     1321 gcgcagtgtg gtcagccttc acaacaatcc
//
LOCUS       DAZGTJ010000271         1340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000271 DAZGTJ010000000
VERSION     DAZGTJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.277"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            56..>1340
                     /locus_tag="ACHK8E_005280"
     CDS             56..>1340
                     /locus_tag="ACHK8E_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0347369.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     VALSVAPEVTAIAEKIRLLDKELRRASVSLKTLKSK"
BASE COUNT          464 a          198 c          288 g          390 t
ORIGIN      
        1 aaaaattata tcccaccact aaggtttata aaagcatacg ttagcaggtg tcaccatgaa
       61 aaaagccata gcatatatgc gattttcatc accaggtcag atgtctggcg actcattaaa
      121 ccgacagaga agacttattg ctgaatggtt aaaggtaaat agtgattatt atcttgatac
      181 cataacatat gaagatttag gattaagtgc attcaaagga aagcatgcac aatcaggagc
      241 tttttcggaa tttttagatg ctatagagca tggttatata ttgccaggaa ctacattgtt
      301 agttgaaagt ctggacagac tttcaagaga aaaagtcggt gaagcgattg aacgtctgaa
      361 attgattttg aatcacggta ttgatgttat aactctttgc gataatacag tctataatat
      421 tgactctttg aatgagccat attcattaat aaaagccata cttatagcac aaagggcaaa
      481 tgaagaaagc gagataaagt caagtcgggt taaattatca tggaagaaaa aacggcagga
      541 tgcactggaa tcaggtacga ttatgacggc gtcttgtccg agatggctct ccttagatga
      601 caaaagaacg gcttttgttc cagaccccga cagggtgaaa actattgagc taatttttaa
      661 actcaggatg gaaaggcgct cattgaatgc aatagccaag tatttaaatg atcatgctgt
      721 aaagaatttc tcaggaaaag aaagtgcatg gggaccttct gtaattgaaa aattattagc
      781 gaataaagct ctgataggta tatgcgtacc ttcatatcgt gcaagaggga aagggataag
      841 tgaaatcgct ggctattatc ccagagtcat atcagatgat ttgttttacg ctgtacagga
      901 aattcggttg gcaccttttg gtattagcaa tagtagcaag aatcctatgc taataaatct
      961 acttcgaaca gttatgaagt gtgaggcttg tggtaatacc atgattgttc atgcggtatc
     1021 tggaagtttg catggctatt atgtttgtcc gatgagaaga ttacatcgat gtgacaggcc
     1081 atcaataaaa agagatttgg ttgattataa tatcattaat gaattgcttt ttaattgtag
     1141 caaaattcaa ccagttgaaa acaagaaaga tgctaatgaa actttagagt taaaaattat
     1201 tgagcttcag atgaaaatta ataatttaat cgttgcattg tctgtcgcgc ctgaagttac
     1261 cgctatagca gagaaaataa gactattaga taaggaatta cgaagggctt cggtatcatt
     1321 gaaaactttg aagagtaaag
//
LOCUS       DAZGTJ010000272         1328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000272 DAZGTJ010000000
VERSION     DAZGTJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.278"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            65..1084
                     /locus_tag="ACHK8E_005281"
     CDS             65..1084
                     /locus_tag="ACHK8E_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0347370.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDENTGEVVDKQLRGRDFLEYFSNR
                     NPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRRQLVKFRTMQTNSLHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLITVLENQYNRLNELDLLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAIMAHDRKYVRNHV
                     SIRPY"
BASE COUNT          381 a          306 c          368 g          273 t
ORIGIN      
        1 atttccggca tcatagtgcc caatacggga tgcagaaagc tccctcagac tggagactcc
       61 acaaatgaaa tatacaccgg ttggcgttga tatcgcaaaa catctgattc aggttcactt
      121 cattgatgag aacacaggtg aagtggtgga taagcaactt cgcggcagag attttctgga
      181 atatttcagc aaccggaacc cctgcctgat tggtatggag gcctgtggag gctctcagca
      241 ctgggcacgg gagctgacaa agctggggca taaagtccgc ttgttacagg cccgctttgt
      301 taaggcattc gtcatgggaa acaagaacga cgtgatggat gcccgggcta tctggatggc
      361 ggttcagcag cctggtaaag aaattgccgt aaaaacagaa gaacagcagt cagttcttgt
      421 gttgcaccgg acccgcaggc aactggtgaa gttccgcacc atgcagacta actcattgca
      481 cggcacgctc ctggaatttg gtgaaaccat tcacaaaggt cgggcagcga tggagcgaga
      541 gtttcctgaa gcactggagc ggatgaaaga gagactgcca ccgtatctca ttactgtgct
      601 ggaaaaccag tacaaccggc tgaatgagct ggacttactg atagaggata ttgaaaaaca
      661 gcttaccagc gtggcgaggc agaatgaaac ctgtaagcgg ttgctggata ttcccggcgt
      721 tggaccactt attgcgacgg cagcggtggc caccatgggg gaagcatcag cgtttaaatc
      781 agggcgagag ttcgccgcat atgtcggtct ggttccaaaa caaaccggct ccggaggtaa
      841 agtacgcctg ctggggataa gcaaacgtgg tgacacttat ctcaggacat tatttatcca
      901 cggagcaaga gcggtggcat tagtagctaa agaaccaggc ccgtggataa ccgaactgaa
      961 aaaacgtcgt ccggccagtg tggcaatcgt cgccatggca aacaagctgg cacgaacagt
     1021 atgggcgata atggcccatg accgtaagta tgtcaggaac cacgtcagta tcagaccata
     1081 ttaatcgctg ataccgttaa acaatgaact cttaacaaaa gggtgaatgc tgaaaggttg
     1141 ctatggcggc ccgagtgatg acaacgacag gtaagaccgt gacctgataa acctgaataa
     1201 gctcctggac ataaagtccg taatgggaat aaggctcagg tcagcgaatc tcatagtggc
     1261 ccgcagctct gactgcaata aggccggata cagagctgca actactcgtc gatgtcagaa
     1321 caacggaa
//
LOCUS       DAZGTJ010000273         1311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000273 DAZGTJ010000000
VERSION     DAZGTJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.279"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..519
                     /locus_tag="ACHK8E_005282"
     CDS             <1..519
                     /locus_tag="ACHK8E_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HFP0347371.1"
                     /translation="SGMQISRHSLVSSYLALMEFSGNAMTRDASRAVLRFVTVTAEAL
                     RFRQIQREFRLALSETAPVYTMTPEEVDLTLNWGRISNVLPEFRGEGGVRVGRISFNN
                     ISAILGTVAVILNCHHQGARSVRAVNEEIQPECQITGDRPVIRINNTLWESNTAAAFL
                     NRRAHSLNTSGE"
     gene            532..795
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005283"
     CDS             532..795
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0347372.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          355 a          276 c          330 g          350 t
ORIGIN      
        1 tccggaatgc aaatcagtcg tcactcactg gtttcatcat atctggcgtt aatggagttt
       61 agtggaaatg ccatgaccag agatgcatcc agagcagttc tgcgttttgt cactgtcaca
      121 gcagaagcct tacggttcag gcaaatacag agagaatttc gtctggcact gtctgaaact
      181 gctcctgttt atacgatgac accggaagaa gtggacctca cactgaactg ggggagaatc
      241 agcaatgtgc ttccggagtt tcggggagag ggtggtgtca gagtggggcg aatatccttt
      301 aataatatat cagcgatact gggcacagtg gcggttatac tgaattgcca tcatcagggg
      361 gcgcgttccg ttcgcgccgt gaatgaagag atacaaccag aatgtcagat aactggcgac
      421 aggccagtta taaggataaa caatacgtta tgggaaagta ataccgcagc tgcttttctg
      481 aatcgcaggg ctcactcttt aaatacatcc ggagaataac aggagttaaa tatgaagaag
      541 atatttgtag cggctttatt tgcttttgtt tctgttaatg caatggcagc tgattgtgca
      601 aaaggtaaaa ttgagttctc taagtataat gagaatgata cattcacagt aaaagtggcc
      661 gggaaagagt actggactaa ccgctggaat ctgcaaccgc tactgcaaag cgcacagtta
      721 acaggaatga cggtaacaat caaatcaaat acctgtgcgt caggttcagg atttgctgaa
      781 gtgcagttta attaatatca gaagcattgc tggtttcgtg gtgtgcagca atgtagttac
      841 agtgtaatca atgtcacaat tcagtcagtt gaaggttgtc tgcccgactg agaatttgtt
      901 aaaaaaatcc tgcatggtga atccccctga gcggaggggc atatcagcgt cacaggtgtt
      961 tctgttttac ctctatcctt tctgtgcggg ttcaggtgct gatactgaac tcaccgggag
     1021 gcacccggca ccatgcatga acggtacata gcgcatacac cagcccctct ccggaggggc
     1081 tttcttgtgg gcaaaaaaaa gcccgagtgg gttcgggcaa tagcatgaga tatacatttt
     1141 tataatcgaa tggattttaa ccaggattca taaggctgcg caactgcgcg gcctttttcg
     1201 tatttcgggc tgtagtcttc cttctgccat tgtcctgtaa cttccggact tcagcccgcc
     1261 ccttatctga ctcacaacat tatcccggcc gggaggattc atggcattta a
//
LOCUS       DAZGTJ010000274         1305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000274 DAZGTJ010000000
VERSION     DAZGTJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.280"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(176..574)
                     /locus_tag="ACHK8E_005284"
     CDS             complement(176..574)
                     /locus_tag="ACHK8E_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347373.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNAGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            complement(722..>1305)
                     /locus_tag="ACHK8E_005285"
     CDS             complement(722..>1305)
                     /locus_tag="ACHK8E_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP0347374.1"
                     /translation="KQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKS
                     HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCIAVSFRSIIAEQKKEPEMTVRYYISS
                     ADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILT
                     NDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          323 a          293 c          235 g          454 t
ORIGIN      
        1 atttagtaca aaaaagcagc gttgcgtgat ctttccctag tccctgtccc ggattgtagt
       61 accggtgcca gttgtagtgc agttcggatt cgtcgtcata ctgctagccc ggatggcgga
      121 gcagttgctc tagtcactca gaatattgta atagtgatag agtgcagtgc cggtttcaat
      181 atcctaacgc tggtcttttg cctgtattaa ctgatttagc tggtttgcta catattaatg
      241 ccttcagatt ttccctttcc ttgtctgtga tactgtcgca aaccctacta gcccattcta
      301 cttcactctt ctccaggcat tctattttct tattgctcat tgttctaatg tttggtgttg
      361 catctccatt taataacatc catgtttcat ttttcttcat aaaacatctt ccattatgtt
      421 caaagatatt aggtgtgaat atattatcag aatcccatgt tacgacttgc tcgagggcta
      481 tattatcttg atagtccatg ccagcattat aataatcaca agatataatt atcatggaaa
      541 taattatcac taatatcttc ccaagcattt ccatttactt tacctcttga aattcaaatg
      601 ggaatccagt gaaaggacct ttaggcataa tatatctttg atatccataa agctcaccga
      661 gcacttgtag gcttataggc gcatttgtat tcatagaggg gttgttaaac ccagggaaag
      721 attacgaaag cccgctccct gcaaggactg acgccaggta gtttctgtcc atggctgctt
      781 ttcgcatctt acgtcttaac cctgccttga ataccttatc attcgttaaa atattaatag
      841 cgatgtgccg tatccctgaa aataattctg cggcgtttcc tcttcttatt ttgcagtcgt
      901 cttcattcat taccacgtcc agacgccagt gcagcttatt ctccacgtgc cagtggtttc
      961 ggattgctgt ggcgaacttt tctgcggtta aatcagcaga actgatataa tatctgactg
     1021 tcatttcggg ctctttcttt tgctctgcta ttattgagcg aaaggagact gctatgcata
     1081 atttcttcag ccctttccat tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa
     1141 caatatgaag tcggatttct tctctgccgt gactcttttc actaattgcg taactgtcat
     1201 gctctggatt atttaattct ttcagtggga atttttcctc aaaggcttta ttaagccgcc
     1261 cctgatttcc ttttacagcg aataaataat caccgccctg ttttt
//
LOCUS       DAZGTJ010000275         1301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000275 DAZGTJ010000000
VERSION     DAZGTJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.281"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1004
                     /locus_tag="ACHK8E_005286"
     CDS             <1..1004
                     /locus_tag="ACHK8E_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP0347375.1"
                     /translation="KLGFRTEIAEPTSFDYLDGKKQPTGFFRSLINSLYEAAIDDSRT
                     SHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFKKDD
                     AIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPSLWHMHPIMFLASLIQP
                     NEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGVKFTDFSKHPNIRHEANGVVSTAA
                     GAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALIAGRKALDVVMQGKISEAIHLCRI
                     EWASLPGSPHAQPTANKKMIMEKYELYLAEEKEGKTSLHATTEKIVKFIEGNYPEYL"
     gene            1001..>1301
                     /locus_tag="ACHK8E_005287"
     CDS             1001..>1301
                     /locus_tag="ACHK8E_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347376.1"
                     /translation="MKKFIFLLLIPVFCNANTPTVFKSSCSGIQYSIESFCSVNNLND
                     DQDKLTIPICSKQIFTLGKENHDLSESTGFVERINIKGEKIKMMNFVFYGATCKSD"
BASE COUNT          429 a          246 c          263 g          363 t
ORIGIN      
        1 caaagctggg gttcagaact gaaatcgcag agcctaccag ctttgattac ctggatggaa
       61 aaaaacagcc cactggattc ttccgcagtc ttatcaactc actctatgag gctgcgatcg
      121 atgattcgcg aaccagtcat gcgctggtga agcataatta tcagcgcctg ctggataaaa
      181 ttgatagtgg aagcgatcgt tactctccaa tggaatactg gagagcactt cataatcccg
      241 actatcgtga cgttcttcag aaaacaatcg ttaaacatcc cagcgactgg tacttcaaaa
      301 aagacgatgc aatctggcaa ccatttctta atgcgctgaa aaaagatgct ccggaatgga
      361 agaaatacag cgaagatttt cttgataaga tggcctggat gcaggatgtg accacggaga
      421 aactgggccc ttcgttgtgg catatgcatc caataatgtt tttagcctcc ctaattcaac
      481 ctaatgaaga tattagaatt cttcgtttac gtgcatttct acgaatgatc cgcatcggtg
      541 aagggacaat tcaggaagac ggatacagaa cgatgtttac tggtgtaaaa tttacagatt
      601 ttagtaaaca ccccaatatc aggcatgaag ctaatggtgt tgtttcgact gctgctggag
      661 cttatcaatt tttatacgga acatggagaa atttgcaacg tagatattca tttccagatt
      721 tcagtccgag caatcaagat ctcggttgta tagcgctcat tgcaggtcga aaagcattag
      781 atgtagtaat gcagggtaag attagtgaag ccatacattt atgtcgtatt gagtgggcca
      841 gtttacccgg ttcaccgcat gctcaaccaa ctgcaaataa aaaaatgatt atggaaaaat
      901 atgaactcta cttagctgaa gaaaaagaag gtaaaacaag tttacatgcc accacagaaa
      961 aaatagtaaa attcattgaa ggtaattacc cagagtacct atgaaaaaat tcattttcct
     1021 gttactaatt ccagtgtttt gtaatgcaaa cactcctact gttttcaagt cttcttgcag
     1081 tggtattcaa tacagtatcg aatcattttg tagtgtaaat aatcttaatg atgaccagga
     1141 taaacttacc ataccaattt gtagcaaaca aatatttacc cttggtaagg aaaatcatga
     1201 cctatcagag tctacaggtt ttgttgaacg tattaacatt aaaggcgaaa aaataaaaat
     1261 gatgaatttc gttttctacg gggccacatg taaaagtgat a
//
LOCUS       DAZGTJ010000276         1278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000276 DAZGTJ010000000
VERSION     DAZGTJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.282"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..269)
                     /locus_tag="ACHK8E_005288"
     CDS             complement(<1..269)
                     /locus_tag="ACHK8E_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0347377.1"
                     /translation="MLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQE
                     GLVRTQEMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMN"
     gene            complement(242..946)
                     /locus_tag="ACHK8E_005289"
     CDS             complement(242..946)
                     /locus_tag="ACHK8E_005289"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347378.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETDST
                     AVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPEASNGVAVGSWASADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGCFPRETCWRRMKSA"
BASE COUNT          344 a          357 c          269 g          308 t
ORIGIN      
        1 ctgttcatac gacgctcatg cgcctgcagg gtacgtgcct gagaagcaat ggcgcgactg
       61 ttgcgttcac ccacctgacg gttacggctt accgcagcac ggttttctgt ggccatttcc
      121 tgtgtacgta ccagcccttc ctgtacagta attcgttcac cggttccctc accgcgcgtc
      181 acataaccat gactgtccac agcaacggta cttccggtat ttatctgctg ttgctggctg
      241 ttcaggcgct cttcatgcgt ctccagcacg tctctctggg aaagcagccc ctggtgttac
      301 ggtttatggc caccgtgttc cggataatat tctccctgtt ctctgcagca accgtatcgg
      361 tatggtcacg cgccttaagt gcttccgctg caatccgtcc actgacgtca ctgtacacct
      421 catccgcctt ccggttcagt tgcccgacag tggctgcatc cgtatcccgt gtaccatcac
      481 tgacaccacc gagaacccgg gttttatccg gtacacgcgg actggccggg tttgtgacac
      541 ctgagtcata tccgatgtag acttcatttt cacgggttgt accggactgc tgaccaagag
      601 ccactgaata cctgtgcatt gcctgtgcac ccgtacccag tgctactgaa tttaaagcat
      661 ctgcagatgc ccagctacct actgccacac cattagacgc ctccggattt acataagcag
      721 acatccccac ggatgtggaa aacggcgcat gcgcatacga tgcagagcca agagctgtcc
      781 ctgccttccc ggcattcgcc catgccccca ctgccgtgga gtcagtctct gatgcagagg
      841 ctgtgtccgg agacgcccag gaactgccat cctgcgcaaa cgccggagca ccggcaatca
      901 gaaaggaaat aagcgataaa gtaataccgt gtcgatttat tttcataaac aacctcattg
      961 atatacacaa tatactggca cagagttaaa ttaatatata atcaggagag aagtgaaaga
     1021 gtacccggtg cgaaaatgaa ttaatctgag cagatttaac ggtaaacgaa gcgcaataaa
     1081 ttaaaattaa aaaacaaaat cacttgcagt ctattatctt tacgttcctc accgcaaccg
     1141 cacacaaaaa caaaacacac aatgcattga ttttaaacaa aaaaacaaaa cagaaactca
     1201 acacataaaa atcagacaat ataaacagaa ttgtgattta tatcctcaag cgctgtatta
     1261 acgaaatata tctgtcct
//
LOCUS       DAZGTJ010000277         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000277 DAZGTJ010000000
VERSION     DAZGTJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.283"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..213)
                     /gene="mchB"
                     /locus_tag="ACHK8E_005290"
     CDS             complement(<1..213)
                     /gene="mchB"
                     /locus_tag="ACHK8E_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP0347379.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSA"
     gene            complement(241..450)
                     /gene="mchI"
                     /locus_tag="ACHK8E_005291"
     CDS             complement(241..450)
                     /gene="mchI"
                     /locus_tag="ACHK8E_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP0347380.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            864..1109
                     /locus_tag="ACHK8E_005292"
     CDS             864..1109
                     /locus_tag="ACHK8E_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347381.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          435 a          259 c          235 g          340 t
ORIGIN      
        1 agcagaagaa ctggcactac cacttcccac ggtcgagcca attgctgttg tcaaaccggc
       61 agatacggct ccaactacaa ccccaagtgg accaccaggt attctggcga gagctccaac
      121 aatagctgca gcacctgcag cattagctga agtcgctggc gcacctcccg ccccggaaat
      181 atatcttaac tgtgattctg ttatttctcg catataaact gcatataaac tccatcatat
      241 ttaacttccg tttttccagc ctgaccagac cagaatcctt cgaatcccat gaatcagctc
      301 aataaaacca agaaatatta taaagcttag aaaaacgtca acataagaat tcccttcgcc
      361 aacaatccga agggatgaaa gtgaaaccaa taagatagta ataggtaaac tgaatatagc
      421 cgtcaattgg gacagttttt tataactcat taatccctac ctgttgcttt attttcatta
      481 tgaaaagtta ccaaatctta gcacctcagt caggagagtc aggtcatcga taaagaatat
      541 tccagtagag tggcgctcat gatcaaagat aacagcaaag ttgatgacag cgctgaccga
      601 ctgtcattta cagaaaccct gtttgtagca cattccatct tacctgaccc agacaaagac
      661 aaagacaaaa gagaatgatt gttatttact tttcaaaaaa aagtgtcata ttgtccatat
      721 aaagtcaaga ggtattttac gttatgtcac tgatgcaatt gttagtcagg atctcatggt
      781 tgtcgctcat gtataacctg agacttgaac gcacactaat agcactgacg gtcatacatc
      841 gggaaatgtg aggaggcata agaatgaatt gtgataataa tcacagaaat gaagaattca
      901 ttgttacctt tgataaaggc aacaagcaag acaattcaag acgaaaacac gataattttc
      961 ctatagaggt agaatcctcc gtagagcagg agacacactg tatcacaaat aataagtcgg
     1021 cttccggtat agtaacacat gactatgatg ccgattatat ttgtggttgt ggtgaaatta
     1081 tgtgtcctgg ttgcggtcat gacctataac aaacattttt gcaaactgat acctgatcag
     1141 gtacaagcct gttgattatt atgctgaata tttcatgtta taaaaccatc gatataaaca
     1201 ttaacaagaa aaaacatata ttgtttatct gaacatttat cgatggaaaa ccaaagggga
     1261 aagtatgta
//
LOCUS       DAZGTJ010000278         1253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000278 DAZGTJ010000000
VERSION     DAZGTJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.284"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            52..486
                     /locus_tag="ACHK8E_005293"
     CDS             52..486
                     /locus_tag="ACHK8E_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFP0347382.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            490..804
                     /locus_tag="ACHK8E_005294"
     CDS             490..804
                     /locus_tag="ACHK8E_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347383.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            888..>1253
                     /locus_tag="ACHK8E_005295"
     CDS             888..>1253
                     /locus_tag="ACHK8E_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0347384.1"
                     /translation="MSEWRKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKII
                     ERLYHQSCIGAVRWGNLPDN"
BASE COUNT          316 a          267 c          361 g          309 t
ORIGIN      
        1 cctcacgggg cagtgctctc tgttatttca ggattacaga ggaactgagt gatgaacgat
       61 aacgcactgg ttaatgttga cgatgctgtt gtggcccgta tcagggatgt gctggcgtat
      121 acatttccgg gacagaaatt ttctgtgcgg aaatgcgatc catctttccc gaaagttttc
      181 aacaatgtgt gggtgagctg gctggatggt ccaacggagg ccgaagtacg ctctgtcaca
      241 gaggggtttg agaataaaga cgggctgctg tctgtgcttg aatcccgtta tttcagcgat
      301 gaactggtac aggagtgtat tgatcgcctt cgtaaagagt acggacaggg gaacgtcccg
      361 ttgagtgtga ccgtggagcg ttaccggaaa aacgagctgg agaagattga aacagcgcgt
      421 tttccgggca acatatccgt agccataaac gagatcggga taaataccag caaatacagg
      481 aggtaaggta tggctggtaa atgccttgtc tggttctgta acgggatctc tgaaccgtgt
      541 ctgatagcag gaaatatcag tgaattactg gcagatgtta cgatcacgtc aaattaccgt
      601 ggagatgtca catctgaaat cagggatgtt gtacaggagt ggctcaggac cggcaggggg
      661 aacctggcgg atttaaagga agagctctgg tactacaacc ttttcatcaa tccgtctgca
      721 gacgaactga tgaatgtcaa caggcggtat ggccctgagc gaactgcctg gctgcagggt
      781 tttatccgca acaacggggc gtgatacccc gttttcccgt gagggcattg tacctcacgg
      841 gacaatgccc tctttttaca tctgtataaa aaagaggagc gtaaacaatg tctgaatggc
      901 gcaaaaaccg ttttgaaatt accggtaaat cagtctgtct tgatgtactt acacagtgga
      961 ttgaagggcg tgagaccccg cgctaccgtc atgccataca gcagagtata cagctgtttc
     1021 tggccggttg tgcagggctg gtaaaaccca cacggacgac gcagtatccg ccatatccgg
     1081 cgctggttcg tcacggaacg gggttgtcat ctccggctaa ccaggcgttt gagcagtggc
     1141 tggggttgct ggtaaaaaat gccgttctgg atggtgaaac gataaagatc attgagcgtc
     1201 tttatcacca gtcatgtatc ggcgctgttc gctggggtaa tctgccggat aat
//
LOCUS       DAZGTJ010000279         1242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000279 DAZGTJ010000000
VERSION     DAZGTJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.285"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1242
                     /gene="tssC"
                     /locus_tag="ACHK8E_005296"
     CDS             <1..>1242
                     /gene="tssC"
                     /locus_tag="ACHK8E_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0347385.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLLQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFE
                     FANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRVAH
                     YLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDGKVVVEDIE
                     DNPGFFRVKLYAIPHFQVEGM"
BASE COUNT          270 a          294 c          357 g          321 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agccgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgctccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaaccat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc ggaaacgcgg gagtttgaat tcgctaatct
      841 gggttttatt ccgctctctt actacaagaa ccgggattat gcctgtttct tctccgctaa
      901 ctccacccag aaaccggcac tgtacgatac gccggatgca acggcgaaca gccgtattaa
      961 tgcccgtctt ccctatattt tcctgctgtc ccgcgttgcg cattacctga agatgattca
     1021 gcgggaaaat atcggtacca ccaaagatcg gcgtctgctg gagctggagc tgaatacctg
     1081 gctccggggc ctggtgacgg agatgaccga tccgggcgat gagcttcagg catcgcatcc
     1141 gctgcgtgac ggaaaagtgg tggtggaaga tattgaagat aacccggggt tcttccgtgt
     1201 gaaattgtat gccattccgc atttccaggt ggaaggaatg ga
//
LOCUS       DAZGTJ010000280         1239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000280 DAZGTJ010000000
VERSION     DAZGTJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.286"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            353..>1239
                     /locus_tag="ACHK8E_005297"
     CDS             353..>1239
                     /locus_tag="ACHK8E_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP0347386.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQ"
BASE COUNT          307 a          291 c          309 g          332 t
ORIGIN      
        1 attaaatttg tttgttttaa aatttacctt gtgaaaattt tctccataaa ctgcaaggat
       61 tgtaagggtt tttcagcttt tcaataaaat tgaagtatct cacattttta ttatcttctg
      121 tcagcgcgga agataatggc ggtagcttgg atcaattccg gtgtctctgc atttcaattt
      181 tactcgatat tttacccggt tgcatcatga tgcctgatta tatttcccgc gtgtcccgga
      241 ggttcgggtg aagagaatat tatctttctg ttttcttttc tctgtccaca acgtgttcgc
      301 ttctccactg ccggccctcc tgagggatgc tctcattcat gatccatcag tgatggaggc
      361 aagggcagaa gccacctctg cccgttcccg tgtggagcag gctcgttcag cacactggcc
      421 ggtcgtcaca gcaacaggca gtaaactcct ttcccagagt caccgctacg actatgacta
      481 cgactctgaa gatattttgc ccggtatccg cggtgaaatg aatctgtttg cttcgggagc
      541 tattgaggcg gatgtcagcc ggagtgagtc ggaagccgag tattaccgtt ataaagttga
      601 tgaggctggc gaggaaacaa ttcgctcctt tgtttcatta tatcttgatg cgctaaggga
      661 aaagcaatcc attgcggtac ttgaacagag tctctcccgg cataacgcaa ttcttaatga
      721 tcttaacacc atcagtattc acgataccgg gcgggaatct gaatttgttc aggccgaagc
      781 ccgcaggctg atggtccggc agcagataaa tacccgcacc cgagtgctca aaaccacgct
      841 gggaaaactt tcgacctgga ccacaaatcc ggtaaccgaa gccgatcttg aaaatccttt
      901 ttcccggatg acagagtcca gattgttaac tgattttacg cagacgcctc agaaagggaa
      961 tccgtcgtgg cttgccagtc aggctgatgt agagagtaaa aaagcggcgc tgaaagcgca
     1021 ggagcttgcc cggtatcccc gggtggattt aacgggatca gtgacccggg atgacaggca
     1081 gataggtgtc agtctgtcct gggacctgtt caaccgtaat gccagttatg gtgtcagtga
     1141 aaaggccgcg cagatagctg cagctaccgg acgactggac tctgttgccc gaatgattga
     1201 tgaaaccgga cgattatcgc tgataacggt cagacaaag
//
LOCUS       DAZGTJ010000281         1237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000281 DAZGTJ010000000
VERSION     DAZGTJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.287"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..869
                     /locus_tag="ACHK8E_005298"
     CDS             <1..869
                     /locus_tag="ACHK8E_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0347387.1"
                     /translation="SKTYIFNGGQTPYDDKPLSVTGIDALGLSINKITTNNMEFNGDV
                     AIVSEDSPYLHNGIVFLNGIPFKIIGVIPKMKMDFLDSLGLSKYQSNNQLFVPLSTLF
                     RFKLSSNIDNVKIVLSDKVGENDINTTKKTLGEEKIPEYNIITALDVRTTVDKVLNRF
                     SILTNTIYIMLTLFAAVICIVLCKRTFSSRSTEFALKIIHGIKQNSIILVVIIESMIT
                     LVICLFNSLVASYFIMLWMSQMLSTDIKIRVVMISLSLSGVLLIFLCSNIFFGKLFFR
                     INPIYLIKGRQQ"
     gene            947..>1237
                     /locus_tag="ACHK8E_005299"
     CDS             947..>1237
                     /locus_tag="ACHK8E_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP0347388.1"
                     /translation="MDDYFNKTNIFSSYILEQEHNKIEESNNVASLLPSMSIGLGQYI
                     NNNKRLSTFGDSDIYFSLSQDIFSAYKYKNNKDKLAIQNNLQNLELQRKRYEY"
BASE COUNT          428 a          181 c          203 g          425 t
ORIGIN      
        1 actcaaaaac ttatatattc aatggcggtc aaacacctta cgatgacaaa cctttatcag
       61 tgacaggtat tgatgcgcta ggtttatcca taaataaaat aaccaccaat aacatggaat
      121 ttaatggaga tgttgctatt gttagtgagg atagtccata tttacacaat gggattgttt
      181 tccttaatgg gataccgttt aaaattattg gcgtaattcc aaaaatgaaa atggacttcc
      241 tggacagtct tgggttatcg aaatatcaga gcaacaatca gctctttgtc cccttaagta
      301 cattattcag attcaagcta agcagtaata ttgacaatgt taagatagtg ttaagcgata
      361 aagttggcga aaatgatatt aacacaacaa aaaaaacgct tggtgaggaa aaaataccag
      421 aatacaatat tataacagct cttgatgtaa gaaccaccgt cgacaaagtg ttgaatagat
      481 tctccatatt aacaaacact atatatataa tgttgacatt gtttgctgcg gttatatgca
      541 ttgtgttgtg taaacgaaca ttttcttccc gaagcacaga atttgcacta aaaattatcc
      601 acggtataaa acaaaactcg ataattctcg ttgtcatcat agaatcaatg attacattag
      661 taatttgttt attcaactca ttggtcgctt cttattttat tatgttatgg atgtctcaaa
      721 tgctatctac agatattaaa ataagagttg taatgatttc attatcgtta agtggcgtgc
      781 tgttaatttt tctgtgttcg aatatatttt ttggaaaatt gtttttcaga ataaacccta
      841 tatatttaat taaaggtcgc cagcaatgag atttttatca aagtctgttg ttctgttttt
      901 ttgtgtttat atggtaactc catatgccaa atctcaggaa tgcattatgg atgattactt
      961 caataagact aatatttttt cttcttatat tctggaacaa gagcataata agatcgaaga
     1021 atctaataat gttgcctcac ttttaccaag tatgtctatt ggtttggggc agtatattaa
     1081 taataataag agattgtcta ctttcgggga tagtgatata tatttctctc tttcacagga
     1141 tatattttct gcatataaat ataaaaataa taaagataaa ttagccatcc aaaataacct
     1201 gcaaaattta gagttgcaga gaaaaagata tgagtat
//
LOCUS       DAZGTJ010000282         1235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000282 DAZGTJ010000000
VERSION     DAZGTJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.288"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            119..877
                     /locus_tag="ACHK8E_005300"
     CDS             119..877
                     /locus_tag="ACHK8E_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP0347389.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(930..1037)
                     /gene="yniD"
                     /locus_tag="ACHK8E_005301"
     CDS             complement(930..1037)
                     /gene="yniD"
                     /locus_tag="ACHK8E_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP0347390.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          310 a          280 c          293 g          352 t
ORIGIN      
        1 ctaagttatt cgcgcctttt tataattcgt tacctttcca gggacgctaa tctttgatcc
       61 ttaagaaagt ttccgggacc gcgaaggtct ctttttttgt tgtacggact cttgataaat
      121 gaagcttttg aagacagttc ctgccatcgt tatgttggcg gggggcatgt ttgcgtcact
      181 gaatgcagct gccgatgatt ccgtttttac tgtcatggac gatcctgcca gcgctaaaaa
      241 acctttcgaa ggtaatctga acgcaggata tcttgcacaa tcaggcaata cgaaaagctc
      301 ctcacttacg gctgacacca ccatgacctg gtatggacaa accactgcct ggtcgctgtg
      361 ggggaacgcc agtaatactt cttctaatga tgagcgttct tcagaaaaat atgcggcggg
      421 cgggcgtagc cgttttaatt taactgatta tgactattta tttgggcagg caagctggct
      481 gacagaccgt tataacggct atcgagagcg cgatgtgttg accgcgggtt atggtcgcca
      541 gtttttgaat ggcccggtac acagtttccg ctttgaattc ggtccaggtg tgcgttacga
      601 caaatatacc gataatgcca gtgaaaccca gccgctgggt tatgcctccg gggcttatgc
      661 atggcagttg actgacaacg ctaaatttac gcagggtgtt tccgtctttg gcgcggaaga
      721 cacaacgctg aactctgaaa gcgcattaaa tgttgctatt aatgaacatt ttggactgaa
      781 ggtggcttac aacgtaacat ggaactctga acctccagag tctgcaccgg aacataccga
      841 tcgtcggaca accttatcgc tgggctacag catgtaaaat tatctgccgg accgtgttat
      901 gcggtctggc tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc ccaacgtagc
      961 aacaacatag cgccacagat tgccagtagc accaccacca tcttccagtg ttttttatca
     1021 aagcgtttag tcggcatacc agtgtcctgc atatctcttg tgcgtgataa cggagtgtaa
     1081 caaaagggac aggcatcaag ggattttttg ttattacagc cgtcgggtgg cgttgtacac
     1141 ctgatcactg tttttgcggt ttatgaaaaa aataaaatta tgtatcgata tataaatatc
     1201 tggttataaa tacgtcttta gttcatcagc actaa
//
LOCUS       DAZGTJ010000283         1225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000283 DAZGTJ010000000
VERSION     DAZGTJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.289"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..965
                     /locus_tag="ACHK8E_005302"
     CDS             <1..965
                     /locus_tag="ACHK8E_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HFP0347391.1"
                     /translation="LVHVGDFDDIHNFSMREDDVLYLKYTFEDDTACWGYGIEEGDAR
                     EELSRKNELPFIGCDKQLRTITNSLKDNFSFQYLEAERFGPRDNIPLSQHNYHEGWLG
                     KNGEHTIEVLESLVTRRRLNFSAQEDPRKHEGIINNNVFNHIEAWMSEISPGHRISPR
                     IEARANVAFNVIIPSPGQLTKPINIGFGFSYALSIVTALMLASKNELVIIENPEAHLH
                     PRGQSYLGRLIALSAKAGIQVIIETHSDHLLNGIRVIARTDPTFDASLFTLYYISREN
                     DETSVAKIKINNEGKLSEWPRGFFDQQSQDMYTIMTGSFDHPNI"
     gene            985..>1225
                     /locus_tag="ACHK8E_005303"
     CDS             985..>1225
                     /locus_tag="ACHK8E_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347392.1"
                     /translation="MKAGVFLIPECFDFNTDILSLSSDLDLASKLVVNTRSEFIHFMK
                     SREFDSKIAELLFSTSPDYAGMMSRFYDQHLSKVIK"
BASE COUNT          398 a          191 c          239 g          397 t
ORIGIN      
        1 atttagttca tgtcggagac ttcgacgata ttcataattt cagtatgcgt gaggatgatg
       61 ttttatattt aaaatataca tttgaagacg atacggcttg ctggggatat ggtatagaag
      121 aaggggatgc tagagaagaa ctttctagga aaaatgaatt accttttatt gggtgtgata
      181 aacaattaag aactataaca aactcactta aagataactt ttcattccaa tatttggaag
      241 cggagagatt tggtccaaga gataatattc cattatcaca gcacaattat catgaaggtt
      301 ggcttgggaa aaatggggaa catacaattg aagttcttga gagtttggtc acaaggaggc
      361 gtttaaattt ttctgctcaa gaggatccta ggaaacatga aggaattatt aataataatg
      421 ttttcaatca tattgaagca tggatgtctg agatttcacc agggcatcgt atctcaccta
      481 gaattgaggc tagggcgaat gttgcattta atgtcataat cccttcacct ggacaattaa
      541 cgaaacctat taacatagga tttggattta gttatgctct tagtattgtc actgctttaa
      601 tgctggcatc aaaaaatgaa ctcgttatta ttgaaaatcc tgaggctcat ctacatccaa
      661 gaggtcaaag ctatttagga cgacttattg ctttatcagc aaaagcaggg atccaggtta
      721 ttattgagac tcatagtgat catcttctta atggcataag agtaattgca cgcacagatc
      781 caacttttga tgcttctctt tttactcttt actatatttc acgagaaaat gatgaaacat
      841 ctgtcgccaa aataaaaata aataatgaag ggaaactttc cgaatggcct agagggttct
      901 ttgatcaaca atcacaggat atgtatacga taatgacagg ctcttttgat catcccaata
      961 tttaatattg tagggacata taaaatgaaa gctggcgtgt ttcttattcc tgaatgtttt
     1021 gatttcaata cggatatttt atctttaagt tcggaccttg atctggcttc gaaactcgta
     1081 gtaaataccc gttcagaatt tattcacttt atgaaatcta gggagtttga ctcgaagatt
     1141 gcggagttat tgttttccac atcacctgat tatgctggaa tgatgtcaag attttacgat
     1201 cagcatctat caaaagttat aaaaa
//
LOCUS       DAZGTJ010000284         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000284 DAZGTJ010000000
VERSION     DAZGTJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.290"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1220
                     /locus_tag="ACHK8E_005304"
     rRNA            <1..>1220
                     /locus_tag="ACHK8E_005304"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          339 a          258 c          383 g          240 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 aagtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1141 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
     1201 tgtttattaa aaacacagca
//
LOCUS       DAZGTJ010000285         1214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000285 DAZGTJ010000000
VERSION     DAZGTJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.291"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            44..1200
                     /locus_tag="ACHK8E_005305"
     CDS             join(44..293,293..1200)
                     /locus_tag="ACHK8E_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFP0347393.1"
                     /translation="MSRKIQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          357 a          286 c          308 g          263 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaatcca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 agcaacagcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga
      361 caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac
      421 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
      481 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      541 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      601 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg
      661 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      721 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      781 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      841 acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      901 cactccgatc gtggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      961 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
     1021 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
     1081 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
     1141 cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa
     1201 aaaaagaaca aatg
//
LOCUS       DAZGTJ010000286         1201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000286 DAZGTJ010000000
VERSION     DAZGTJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.292"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..896)
                     /locus_tag="ACHK8E_005306"
     CDS             complement(<1..896)
                     /locus_tag="ACHK8E_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0347394.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKE"
     gene            complement(903..1196)
                     /locus_tag="ACHK8E_005307"
     CDS             complement(903..1196)
                     /locus_tag="ACHK8E_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347395.1"
                     /translation="MYTLLLTGITVFSLWLADTGIRHAEGFAALREWMYETRYGWLGW
                     RLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILLCEYAIFAEPGR"
BASE COUNT          301 a          396 c          243 g          261 t
ORIGIN      
        1 tcctccttat aatccacctg actactgcca atcatcttca ttgcagctga cagacgtaac
       61 cagtaatgat cactgacctc ctccatgacc cgttttttca gccccacact actgtcactc
      121 caccactccc ggcatgtcgg atagcctccc cgccccgtat ccggtcgtcc gctgtcacgt
      181 gctgtattcc acgggaacgc agcccgcggc atctttgact gcagcgtccc gtaatccccc
      241 tgcatgaatg tggcgctccc tatccaggag atatcatcaa gcactttttt atctttactc
      301 tgcccctgat cacgctgttt ccacatatac agtgccagtg aataacagtc gttcgtaaag
      361 tcctgcagct catcagcaag tgccctgttt tctataaacg ttcgctggat ctcaaaacgc
      421 aactggcgca aatcaggccg gcacggaatc gaagccactg ccgcctgggt gacacctttc
      481 gacaatttat gaaccaccac ccaccatacc ggggcagcag cagtctgatc ccctatactg
      541 ctgatcaccc cactgtaccc cgtctctccg ggctcttcag gtgtccatac cccacaggtc
      601 tgtgccctgg atttatcgaa tgacagcgta tccagcgtca cctgtaccag aggaacacag
      661 caggatatca tcacaaaaaa agcaccatag agaacatgct caatacgcgg cagggaaagc
      721 atccctttgt ttccctcatc cgcaccttct tccctgactt tcagccagat cccgatgatc
      781 ctgaatgcca gtggcagggc aaacagtcct gtgctgatca acacattcca cagaccgtta
      841 ttcaccaccc agcccagcag agtcagaaaa tattccagat agttatttgt ggtcatgatt
      901 gctcaacgcc ccggctcagc aaaaatggcg tattcacaca gcaggataaa cagaacactg
      961 actgtggcca tacgcagaaa agaagcccgg tattctgccc tgaaccccgg cgcatgccag
     1021 atcctccacc ccagccatcc gatcgcactg taaagacaca gacgccatcc cagccagcca
     1081 taacgtgtct catacatcca ctcccgcagg gccgcaaatc cttctgcgtg acgtataccg
     1141 gtatcggcca gccagaggga aaacacggta atccctgtca gcaacagcgt atacagggcg
     1201 a
//
LOCUS       DAZGTJ010000287         1079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000287 DAZGTJ010000000
VERSION     DAZGTJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.293"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..382
                     /locus_tag="ACHK8E_005308"
     CDS             <1..382
                     /locus_tag="ACHK8E_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI immunity family protein"
                     /protein_id="HFP0347396.1"
                     /translation="VKVNQVIKPVRFNESRSLHFYSEYEGLQFDKESTMKWYRRFDEA
                     SALLDKEEMGKSCAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKY
                     GKQHRACWKLLKRDDNGSVYTLPE"
     gene            408..787
                     /locus_tag="ACHK8E_005309"
                     /pseudo
     CDS             408..787
                     /locus_tag="ACHK8E_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            784..>1079
                     /locus_tag="ACHK8E_005310"
     CDS             784..>1079
                     /locus_tag="ACHK8E_005310"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFP0347397.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          269 a          236 c          325 g          249 t
ORIGIN      
        1 ggtgaaggtg aaccaggtca ttaagccagt gcggtttaat gaatcaagat ctcttcattt
       61 ctactctgaa tatgaaggac ttcaattcga caaagaatcc accatgaagt ggtacaggcg
      121 ctttgacgaa gccagcgccc tgctcgataa agaagaaatg gggaaatctt gcgcccccga
      181 acgcattaaa tgctggaccg atgaaatagc gccccatgcc ggacaatggg ccgcgatggt
      241 taatggcacc accgagtaca tccagacccg cgaagggcag aaaatgcccc cgtttgagga
      301 taagtacggt aagcagcaca gggcttgctg gaaactactg aaacgagatg acaatggtag
      361 cgtttataca ctacctgagt aaggtcgtgt gctgaatgac cgcgcaggcc ggagagaggc
      421 tggggctttt tgcccggacc ggacaactga gcgtcaagtc cggggagggg ccggtagagg
      481 ttcaggcgaa aaacggcagc atgcggttgt ttgctgagaa gaagctgaca ctgagctcag
      541 cgtgtgatat ctcttttgcc gggaaaaaac gtatcacgct gattggcggg ggagttacct
      601 gcgacttgaa gccgggaagg tcgagtatgg tacgacggca aagtatctgc gcaaagtgaa
      661 gcggacaatg gcggcggggg ctgcatcaat accagtcagg tccattgtgg ggggaggtat
      721 ttgcctgagc tgtctgatga aggcggcaat gaatggcgac acctttgttg taaggggtgg
      781 gtcatgaaca gcgtcagttt tagccttgcg gcacaacacc tgatttgttc aacaaaagtg
      841 acattatttg ctctggtgga tggtctgcaa tatgagcggt atttcggtga gagtctgtct
      901 gttcaacaac ctgccgccgt gcccctgttc gatacctggc ctgattcgcg tattgcattt
      961 gcggggccat gggttatgga gatgaacagc ataatggatt tcagagaaag gctgtgtgag
     1021 cttgaagctg cgcttccgtc agtttcctgg atgatatctt cttcctcgct tactgaact
//
LOCUS       DAZGTJ010000288         1075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000288 DAZGTJ010000000
VERSION     DAZGTJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.294"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1039)
                     /locus_tag="ACHK8E_005311"
     CDS             complement(<1..1039)
                     /locus_tag="ACHK8E_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0347398.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLII"
BASE COUNT          332 a          241 c          252 g          250 t
ORIGIN      
        1 tgataatcag aacaccgggg gttgcgatcc attcaatctt catcgacaga ttgctgccgt
       61 tactcagaga gaagctgagt gacgaggcaa tctgagacac tgtacttttt accgtcggga
      121 acagtggaga gcaaagaaga atgaacagaa aaatcaggat gtagattgag cacgcacgta
      181 acaacgcccc acgagagtat gaaagtgcgc tggcgctatc tgctgaagtc tcaataagaa
      241 attcaggagc tgttttttct ttacggtttt ttgccataaa gactatcacg atcaggctga
      301 ccagacttcc cgcgaaagca ggaagttctg cgccaagata aacagccacc aggtactgtg
      361 ggattaatgt gctcacaccg cacattaagg tcatgaaccc aacgcctcgt atagccttca
      421 ggccttcccc aataatagcg actataacga agggaagcag aatgttgaac gctgccagct
      481 gaagaataat cgtcccgctg agggttgtta cgggtaaatt tacctgttca gcaagtactg
      541 atacaggtat gccgacagca ccaaacgcgg taggtactgt attagcaacc aatgatgcaa
      601 ttgcggcttt aagagggtta aagcccagtg caatgagtat accaatgggg attgctacag
      661 ctgtaccgta cccggccgcc gcctcaagaa atccaccaaa acaccatgag ataagaagca
      721 cctggatacg tttatcatca ctgacagcgg ccagaacatt tcgcagaatg ttcatgcttt
      781 ttgtcgcatt catcaggtta tagctgtaaa tggcagcaag tatgactatc actatcggcc
      841 acagcccttt gattgcgcca tatacaacag cagaacccag atcctgaact ggtgtgtgcc
      901 agaacaaagt tgcaagaatc agtgtaataa agagtgtgct gagtacggca taatgtatgg
      961 gcattttgac tttcagaatc aaaatgatca ttgccagaag tggggtaagt cccagtaaaa
     1021 aaagcaggta ttggtgcatg tcatttcctc aacgaaaaca aattcagcat catta
//
LOCUS       DAZGTJ010000289         1050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000289 DAZGTJ010000000
VERSION     DAZGTJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.295"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1050
                     /locus_tag="ACHK8E_005312"
     CDS             <1..>1050
                     /locus_tag="ACHK8E_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0347399.1"
                     /translation="WPLLTLSQSQLQRLSPLLDAQSDECPERLQDAEPVLPQGDNVTF
                     IGRPVGADTAGIPSGDLPPVLQSALDKFTRYITASAREGKIDPVSGRDTEIRQMVDIL
                     SRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQAGAGVK
                     GEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRT
                     IAATTWSEYRQYLERDAALERRFQMVRVDEPDDETACLMLRSLKSRYAEHHNVHITDE
                     AVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEK
                     QALLEDIAVGHQNHG"
BASE COUNT          214 a          297 c          326 g          213 t
ORIGIN      
        1 tgtggcccct gctgacactg agtcagagcc agttgcagcg tctgtccccg ctgctggatg
       61 cgcagtctga tgagtgtccg gagagattac aggatgcaga gcctgtgctg cctcagggag
      121 ataatgtgac ctttatcggg cgtcccgtcg gtgcggatac ggcgggtata ccgtcaggtg
      181 acctgccgcc tgtgttacag agtgcgctgg ataaattcac ccggtacatc acggccagcg
      241 cgagagaggg gaaaattgac ccggtatcgg gacgcgatac ggaaatccgt cagatggtgg
      301 atattctctc ccgccgccgc aagaacaacc cgattctggt gggggaccct ggtgtgggga
      361 aaacggctct ggtggaaggg ctcgccctgc gtattgcgga aggtaacgta ccggaatcac
      421 tcagacccgt caccctgcgc acccttgacc tcggcctgct gcaggccggt gccggcgtga
      481 aaggtgaatt tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag tcaccggctc
      541 ctgtactgct gtttatagat gaagcccata cccttatcgg tgccggtaat caggccggcg
      601 gcgcggatgc ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg cgcaccattg
      661 ccgccaccac ctggtctgag tacagacaat acctggaacg tgacgcagcc ctggagcggc
      721 gttttcagat ggtcagagtg gacgagccgg atgatgagac ggcctgtctg atgctgcgct
      781 ccctgaaatc ccgttatgcg gaacatcata acgtgcatat caccgatgag gcggtacgtg
      841 ctgccgtcac gctgtcgcgc cgttatctga cagaacgcca gttaccggac aaggccgttg
      901 acctgctcga tactgctgct gcccgtgtgc gtatgagcct cgacacggtg ccggaacagc
      961 tgacccgtat ccgttcacag ctcgcttccc tcggtatgga aaaacaggca ctgctggaag
     1021 atattgccgt tggtcatcag aatcacggcg
//
LOCUS       DAZGTJ010000290         1038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000290 DAZGTJ010000000
VERSION     DAZGTJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.296"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..497)
                     /locus_tag="ACHK8E_005313"
     CDS             complement(<1..497)
                     /locus_tag="ACHK8E_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0347400.1"
                     /translation="MRLTPMGIPGKDPAHVKAWTQQEDRLLISLYQEHNTHQMAERIQ
                     RTRKAVMYRIRFLRNRGLIGRKRKAPLSTEAIAFLIKNRHVKTAQEMALEVGCSECTV
                     RYTLRKRGYNLKKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTIQMHIN
                     DDAAF"
     gene            complement(524..736)
                     /locus_tag="ACHK8E_005314"
     CDS             complement(524..736)
                     /locus_tag="ACHK8E_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347401.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            complement(733..975)
                     /locus_tag="ACHK8E_005315"
     CDS             complement(733..975)
                     /locus_tag="ACHK8E_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347402.1"
                     /translation="MNSIFTATMLTRFTDAVGHEFMVESHLITTTTPCPSDADYLYIH
                     LADGTQITAIASTVREVTAIRGAWKSETQAHGELRP"
BASE COUNT          189 a          264 c          282 g          303 t
ORIGIN      
        1 tgaaacgccg catcgtcgtt aatgtgcatc tgtatcgtgc tgttgatgtg ttcagcgatc
       61 atgcagaacg tcatgttgtg tatgtcgcgt agctccgtga ccagctcaac cagatgatcg
      121 ctgtatctgg cgcaatggtg gttctcaccg cattttttca ggttgtaacc ccgtttacgc
      181 agtgtgtacc tcactgtgca ctcactgcat cccacttcca gagccatttc ctgtgcggtt
      241 tttacatgac ggtttttaat aagaaaggct atggcttctg tactcagtgg tgcttttctc
      301 ttgcggccaa tcagcccacg attacgtaaa aaacgaatcc ggtacataac tgctttacgg
      361 gttcgctgaa ttcgttcagc catctgatgt gtgttgtgtt cctgataaag cgagatgagt
      421 aaccgatctt cctgctgtgt ccacgccttc acatgtgccg ggtctttgcc cggtattccc
      481 ataggtgtaa gtctcatcgc gataacctcc gttactgttc cggttattcg gtcgggttca
      541 gggtgtaatc aatggcgttt tcaatacggc gtttaaggtc ccggtggatc tcacctggtg
      601 ttactggtgc gcccggaccg ctcagcgttg ccgtgaagtc gtcgatttcc tccagtaaca
      661 gattgcggag tacggcgatg tggtggcgtg cgtctttacc gggtttacgt gcgttggtgg
      721 tgtacttgct gatcatggcc gcagctctcc gtgggcttgt gtttctgatt tccatgcccc
      781 tctgatggcc gttacttcgc gaacggtact ggcaatcgcg gtgatttgtg tgccgtcagc
      841 cagatggatg tagagataat cagcgtctga cgggcacggc gtggtcgttg tgatcaaatg
      901 actttccacc atgaactcgt ggcccacggc atcggtgaaa cgcgtcagca tggtggcggt
      961 aaatatgctg ttcatcgacc tgcccctcac gctgaaaact ggccggactg tgcccggagt
     1021 tctgccagtt cgcgctcg
//
LOCUS       DAZGTJ010000291         1030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000291 DAZGTJ010000000
VERSION     DAZGTJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.297"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>1030)
                     /locus_tag="ACHK8E_005316"
     CDS             complement(<1..>1030)
                     /locus_tag="ACHK8E_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347403.1"
                     /translation="IKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTLAG
                     LSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGTDV
                     SGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQESDI
                     PAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDNPL
                     TRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTDPY
                     PFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVRTL
                     PSSDVNGSS"
BASE COUNT          188 a          356 c          232 g          254 t
ORIGIN      
        1 tactgctgcc gttcacgtct gatgacggca gcgtacgcac tgtcccgtcg gcaaaaacaa
       61 acgtgatgct gttcacctgc ccacggacac aggagagcgt ccagtcacca ctcgcagtac
      121 cggacaccac tgcaccttcc acatccggca gctctattcc gtttgccgtc aggttatcct
      181 tacccaccag gatcttgaac ggatagggat ccgtcacggt tccgttcacc ggaacccgtc
      241 ccagaagcgc tgtcatcgcc cggctgcctg ccagcgtcga gttctccggc agggtataca
      301 ctgcccgggt gctcgccttc tgctgttcat tgctgtatcc cttcacctgc tgttcataag
      361 ccgctttctg acgggtcagt ggattgtcat ccagaaaaga gactggaaaa cgcggtccgc
      421 tcttatcccc caccgacgac gcttcatcct gcacctgatc ggccggtgtc acccacatca
      481 gaccatccgt tcccggtgag gctgccgatc ctgtcacgct ccccagtccg tcaaggccca
      541 gacctgctgg aatatcgctc tcctgtcctc cggcgccggc atcagcactc tcctgctgac
      601 gcttcagttg ctctgtcagt gactgaacct gtgtcattaa cgcgttctgt tcattccgga
      661 actggcgctg cgactgctca taatttttct gcaatcctgc cactgcctca ctgacctgac
      721 cggaaacatc cgtgccccgc ttgcgcagca tttcattttc tttcagcaac ttttcattct
      781 gtttgtccag taccagttgc cgctccctca cctgattcat acgcccaatc agtgtgcgca
      841 gggtatcttc aggtgtgtct ccttccacgc ccagggcctt cagttcatcc ggtgacagtc
      901 cggccagcgt tcctgagctt tccgcagacg agggctcccc ggtatctcct ccacagcttt
      961 ttaatcccgt caggatcccc gccaccacca cacagggcac cacatacttc acgatcgtat
     1021 tggatttaat
//
LOCUS       DAZGTJ010000292         1010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000292 DAZGTJ010000000
VERSION     DAZGTJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.298"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            15..320
                     /locus_tag="ACHK8E_005317"
     CDS             15..320
                     /locus_tag="ACHK8E_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347404.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            375..896
                     /locus_tag="ACHK8E_005318"
                     /pseudo
     CDS             375..896
                     /locus_tag="ACHK8E_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
BASE COUNT          252 a          253 c          270 g          235 t
ORIGIN      
        1 acgagggctg aatgatgaac ccgttattta atgacatcca gatgcgcctt ttttatctga
       61 atcatgcacc gtattcctgg cactggaatg tcagattcag gccacaggaa gccgtatata
      121 ttggtaatga tgcctgccat attaccatta cctgtaatca gtccggtttt catttaacca
      181 gggacgggca gcgactgttc actgaacgtt atatcagaac actcagtgag ttattggcgg
      241 ttctgaagcg gcggtgggat gtgacaccgg caatcatccg tgcggtggaa tacctttccc
      301 gggtaccggt atcacactga cgctctttcc gggaatatta attatgattt gatacagcaa
      361 cactgaatct gccgatgact tctgaagtaa aactgaaaac aggtaatgac ccccggtcat
      421 taccggatta tgctgccctg cgcgatgaaa tcagcaagct gactcacccg gcacgtccgg
      481 atgtggactg gcgttatgtg gagacgctct gcctgcgtct gtatgaacac aatggcgtgg
      541 aattacagac ggcatcctgg tacaccatcg cccggatgca tacaacaggg ttaagtggcc
      601 tgaatgaagg gctggctctg attgtggcgc tgacccggca tcactggcca gtgatgtggc
      661 cgctgaatac ccacgcccgg ctggaaatta ttaccggtct gttcagtcgt ctgcaaaaaa
      721 cgctccgggc catgccaccg gatgagcgtg acaacctgcc cctgctgtat cagacagaaa
      781 catgcctgaa aacgctgagt gacatactga cccggcatga acttaaacag agcagtaaag
      841 tggctttacc ggaagccatg gtgaagggat atatcacccg tctggagaat cagccgtaag
      901 cgtacagcga gggccatatt gacggggatg tgttattcag ctggcagtgc tatgcgccac
      961 ggaagcagtt cgctgacccg gttgaccggc cagtctgcta tgacgccaag
//
LOCUS       DAZGTJ010000293         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000293 DAZGTJ010000000
VERSION     DAZGTJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.299"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1007
                     /locus_tag="ACHK8E_005319"
     CDS             <1..>1007
                     /locus_tag="ACHK8E_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0347405.1"
                     /translation="SVDGAVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMC
                     GNFDLKTTVGNQLNGITDGFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGV
                     LQANVAFDKAQLNCQNMAKRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGR
                     QVTGVEGQRWIGGQQRGGAGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSA
                     CMKYRNAEEAARAVVKVLGDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEAT
                     RVNTELLAGMVNGRDKPTAGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELA
                     M"
BASE COUNT          244 a          221 c          352 g          190 t
ORIGIN      
        1 aatccgtgga cggcgcagta tcagacaaac tgttttatac cctgggaggg ggctcggtga
       61 tatcgcagcc ggcaacgcgc agcaatatgc agaaacttgg ggtggatctc gggtggagca
      121 gtgatctgat gtgtggcaat tttgacctga aaacaacagt gggtaaccag ctgaacggga
      181 tcactgatgg tttcaaaaac ctgatggggg atgtgattca gggggcgagc ggagccgtgg
      241 cgagtctgcc ggcaatggtg atacagcggg ctaatccggg gctgtatgac atgctgacca
      301 acggtgtact gcaggcgaat gtggcattcg acaaagcgca gctcaattgt cagaacatgg
      361 cgaaacgcat gatggagttc acggacagca ataagtggac gcaggcggcc atgctcgagg
      421 agtacaaaaa aattgtgaat acgggggacg gtgatgcgat caaaggggat gaagcaggtc
      481 gtcaggttac cggggtggag gggcaacgct ggatcggagg gcagcagcgt ggtggagcgg
      541 gacagtctgc tatccggccg acacatgatc tgacagctgc cggtttcaat atgatgaaca
      601 gtctgccttc gaccagtaca tccagtgtca gtcccgggaa ctgtaacggg agtgcctgca
      661 tgaaataccg taatgcagag gaagctgccc gggcggtggt gaaggttctg ggggataaat
      721 ccatgcggac ctgtggtgat gcaaaggcat gtaccagcgg ggatgctgat cagcagccgg
      781 ggaccagtat tgccggcacg gggtttgcgc cgatgcttga agaagcgacg cgggttaaca
      841 cggaactgct ggcaggtatg gtcaatggtc gtgacaagcc gacagccggg aatctggcaa
      901 aactgaaagc cgggagtctg tctgtgacag cgggagtgat ccgtgccctg cagcgtgatc
      961 cggacaatgg tgccctgacg tcgcgtctgg cgggagaact ggccatg
//
LOCUS       DAZGTJ010000294         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000294 DAZGTJ010000000
VERSION     DAZGTJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.300"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1004
                     /locus_tag="ACHK8E_005320"
     CDS             <1..>1004
                     /locus_tag="ACHK8E_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0347406.1"
                     /translation="WPSLEIRPDRRTDPDPERVPGAVMRLSCATWWNSQLRHLRDIWR
                     EELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIENEEGERFSLEDIYWA
                     GLSNPRNRRNEMMTCARGMEQVAEARGDVAFFVTVTAPSRFHSVNDDGSLNPKYCGAT
                     VRDASDYLVYRFFAAVRRAIDKAGLGWYGVRTVEPHHDGTPHWHMLVFTSQENEARIT
                     EIMRNAAIREDRAELGDDISPRFKCKKIDPAKGTPTSYIATYIGKNLDASTFMGNDPK
                     TGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQFFGIPPRQVWRELRRLAGQMARNP
                     "
BASE COUNT          234 a          308 c          288 g          174 t
ORIGIN      
        1 gctggccgtc gctggaaatc aggcccgacc gccgcaccga ccccgacccg gaacgggtgc
       61 cgggtgcggt catgcgcctt tcctgtgcga catggtggaa cagtcagtta agacacctcc
      121 gggacatctg gcgtgaagaa ctgttacgcg ccgcaggtct ggtgtcacgt aagacatcgg
      181 tatatatcag ccatgaagcg ttactggact ggcgcgaaaa acgccagcgc acccgcgatt
      241 ttctgaaggc ctgggacatc gagaacgaag agggagaacg gttcagcctt gaggatatct
      301 actgggcagg tctctctaat ccgcgtaacc gccgtaacga gatgatgacc tgcgcccggg
      361 gaatggagca ggtcgccgaa gcacgcggcg acgtggcgtt tttcgtcacc gtcacagccc
      421 cttcccgttt tcacagcgtg aacgatgacg gcagcctgaa cccgaaatac tgcggagcaa
      481 cggtacgcga tgccagtgat tatctggttt accgcttttt cgccgccgtc cgcagggcca
      541 tcgacaaggc cgggctgggc tggtacggcg tgcgcaccgt ggagccccac cacgacggta
      601 caccgcactg gcacatgctg gtattcacca gccaggaaaa cgaagcccgt atcactgaaa
      661 tcatgcgcaa cgccgccatc agggaggacc gcgcagagct gggtgatgat atctccccgc
      721 gttttaaatg caaaaaaatc gacccggcaa aaggcacacc gacaagctat atcgcgacct
      781 atatcggtaa gaacctggac gccagcacct tcatggggaa tgacccgaaa accggcaaac
      841 cttacgtcga taaggaatca ggcaaaacca tggccgaaac cgtggagaac gccatcggct
      901 gggcaggcct tcaccgcatc caccagttcc agttcttcgg tatccctccc cgccaggtat
      961 ggcgcgaact ccgccgtctt gccgggcaaa tggcccgtaa cccg
//
LOCUS       DAZGTJ010000295          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000295 DAZGTJ010000000
VERSION     DAZGTJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.301"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..473)
                     /locus_tag="ACHK8E_005321"
     CDS             complement(<1..473)
                     /locus_tag="ACHK8E_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347407.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQ"
     gene            complement(473..817)
                     /locus_tag="ACHK8E_005322"
     CDS             complement(473..817)
                     /locus_tag="ACHK8E_005322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347408.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            complement(811..>996)
                     /locus_tag="ACHK8E_005323"
     CDS             complement(811..>996)
                     /locus_tag="ACHK8E_005323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347409.1"
                     /translation="MNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWL
                     TIVDGKAWSFKQREFIC"
BASE COUNT          225 a          300 c          181 g          290 t
ORIGIN      
        1 tgttgcgtct ccggattata aaacgtgcca catattcttg tctggtagct gattgttacc
       61 atactccagc gcgagcaacg ccttatcatc ggcattacct tcccttccgg tgggcagttc
      121 tcacacgttt tctcatccgt cttcgcctgt acttcggctt tgctatcaat accttcatag
      181 tttactgttt ctgaatgatc catatcatct tcccagaaat caccatgcgt ctctgcgctg
      241 acaaccatcg gctcattcag aattggatca tactgtttcc cctgataata aaaccctccg
      301 ttaccctgac gcgaaccact gcctgagcca ggataagata tattatcttc ttcccagtct
      361 ccatcgcggc ttcctgctcc gggaaatact gctgttccgg ttgaggcacc agccatacca
      421 acacctccgg tagctgcgct ccccatacca gcccctgtca ggaccataac catcagagct
      481 ccctcctttc tctgtttatc atcacatgct tataatcttc aacccgctgg tcagttgaat
      541 aagcattcgg cgttctcaac catttatcca cttcgggttt cagataaaaa ccaggccaga
      601 aggcctccag aaacaagaag tcccgcttca cgtttttctc tttaagcgga agggtattgg
      661 cataatccag cgcttttctc aggcgaacat ggaacgtgtc atctgattca gtaattcccg
      721 gaaatttctt cttcagtctt agcgcggtgt cgcgaacaaa atcatcatct tcccttttgt
      781 ccataccaac acactgctct tcagacaact tcagcatatg aactcccttt gtttaaatga
      841 ccaggctttt ccgtccacta ttgtcagcca ctcctgcatt aaccccgtca gctcccgatg
      901 ttgttggtcg ttcagcattt ctcccaggcg cttctgaacg cgcggatcat ggaaacgcag
      961 caacgcaatc cgtccatccg gtagttctgt attcat
//
LOCUS       DAZGTJ010000296          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000296 DAZGTJ010000000
VERSION     DAZGTJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.302"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHK8E_005324"
     CDS             complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHK8E_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0347410.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKE"
     gene            complement(174..377)
                     /locus_tag="ACHK8E_005325"
     CDS             complement(174..377)
                     /locus_tag="ACHK8E_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347411.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(468..935)
                     /locus_tag="ACHK8E_005326"
     CDS             complement(468..935)
                     /locus_tag="ACHK8E_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP0347412.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT          245 a          258 c          222 g          269 t
ORIGIN      
        1 tgttccttca tttcaccgct ctgtttatca cgccaggtat cagtggtggc cagcgtcaga
       61 tttgccactg cacctccgcc gttggggaaa taacggattt ccggatcctg tcccagatga
      121 ccaatcagta tgactttatt cactccacgc gatgccataa caactcctta tcctcacaca
      181 cattcacgct gagtccagat gccgttttcc agagcccgga ctgcctgctc ataatttctg
      241 aaatactctt cggattcccg ggacacaggc ccttcgtcgc actgagtacc aatataataa
      301 ccacgagcac tgctgaggat ctgcagaggt aaggtatagc ccgctttggc ggccagaatt
      361 ccgcttgtcg gtttcataac atgtttcctt aaagttaaac gataagaaaa cagacctccc
      421 tgatggttgg tctgttttca tcagggaggt tgagttgaaa aatacgatca gaaagaattt
      481 tctgcgtatt cccgcggggt attgccattc tgtggcggta tggattccga tttttcagtc
      541 ttataaacca tgtcctggcc tattttgatc cactccacct tgatcaggcg ggctttcaga
      601 ctgacgcgtt gctcaccggc atgttcaccc cggttaaggg taaaaatgtc agtagagggg
      661 ttgctcatgg taaaaccaat cagcaccttg cgatcttcat ccaccgcttt ctggcagcgg
      721 gcaatcaggg aagttgcctc tttaccggca accgtcacat caaaacgcac ataggacggg
      781 ctgtccgtcg gaccactcag tgcattcaca acacaactga gaaatgtccc gttctgatga
      841 ttaacctgac gaatgttgct gagatacccc aggcctttca gtgtcagatt gaagtactca
      901 gttttagcgt ttgcagaaga agtatttgca gtcataatga tatctccatt ctcagtatcg
      961 gaaagacaga gaacatcatc atccctgacg ggtt
//
LOCUS       DAZGTJ010000297          993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000297 DAZGTJ010000000
VERSION     DAZGTJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.303"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>993)
                     /locus_tag="ACHK8E_005327"
     CDS             complement(<1..>993)
                     /locus_tag="ACHK8E_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFP0347413.1"
                     /translation="ILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESIT
                     PVTNTIPGEEDDDEPVFSEDAVSEHLRFRYGRRWQHKVRLLLVMGDPDVVRKAAPGLC
                     HDLWQEGDGYVLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALPTDGERD
                     ALLRYRQKADHQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVKGADEAFST
                     LALHLQKAGMAQILDEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMF
                     SPELTVAGAVPNTCPDSPAWKAVIDDCGAASGRKLGFSWLKMLRLILLSLIVLWG"
BASE COUNT          217 a          310 c          264 g          202 t
ORIGIN      
        1 cccccacagg acaatcaggg aaagcagaat caatcgcagc atcttcagcc agctgaagcc
       61 cagttttctg ccgctggcgg caccacagtc atcaatgacg gctttccagg ccggtgagtc
      121 ggggcatgtg tttggtaccg ctccggcaac ggtcagttca ggactgaaca tgacaccgcg
      181 taaacgccag gcagcagtcc cctgcatcag tccactgagc aaaacagcca ggctggcttt
      241 cagctcctgc cgcagtctga gtgacaactg aaggagaccg aaatcagcag gctcatccag
      301 tatctgcgcc attcccgctt tttgcaggtg taacgccagt gtggaaaacg cttcgtccgc
      361 acctttcaca gttccttccg gtccgaagat gacccctgtt gcggccgctt tcgcgtcctg
      421 ccgggcaccg gtagcgttgt cagtcagcca cagccagact ggtgcctgcc agcccaactg
      481 atggtcggct ttctggcggt aacgcaggag ggcatcccgt tcgccatccg tcgggagggc
      541 tgaaatgctc atcacctgaa tgatgccatc aaccggttgc ccggatcgca gacgcttcag
      601 ttttgaaagg aagatctcgt ccgggagtga ctgcgcgtct ccgccataaa tcagcacgta
      661 gccgtcgcct tcctgccaga ggtcatggca aaggcccggg gctgctttcc ggaccacatc
      721 cggatccccc atcaccagca ggagtcgcac cttatgctgc cagcgacgcc catagcgaaa
      781 gcgcaaatgt tctgaaacag catcttcgct gaaaaccggc tcatcgtcgt cttcttcacc
      841 gggaatggtg ttcgtcaccg gagtgatact ctcagccttt ttctgccccc actggaaata
      901 cagcccggat ttaccggcga agtcgaaagc cttctccgtc agccagccga gtaaaaacag
      961 cgtggcaacc agtatggcgc agaatgtgag tat
//
LOCUS       DAZGTJ010000298          962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000298 DAZGTJ010000000
VERSION     DAZGTJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.304"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>962
                     /locus_tag="ACHK8E_005328"
     CDS             <1..>962
                     /locus_tag="ACHK8E_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFP0347414.1"
                     /translation="LDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYR
                     YDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQYAEPLVESRYLYDP
                     LGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTVYQPGSFT
                     PLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVS
                     EESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEY
                     DEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQG"
BASE COUNT          215 a          295 c          306 g          146 t
ORIGIN      
        1 ctggatatcc gcatcccgta tgccacggac ccggcaggta accgcctgcc cgacccggag
       61 ctgcacccgg acagcaccct cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac
      121 tatctttacc ggtatgaccg tcacggcagg ctgacagaga aaaccgacct catcccggaa
      181 ggggttatcc gcacggatga tgagcggact caccggtacc attacgacag ccggcaccgg
      241 ctggtgcact acacgcggac acaatatgca gagccgctgg tcgaaagtcg ctatctttac
      301 gacccgctgg gccgcagggt ggcaaaacgg gtatggcggc gtgaacggga cctgacgggc
      361 tggatgtcgc tgtcacggaa accggaagtg acctggtacg gctgggacgg cgaccggctg
      421 accacgatac agaacgacag aacccgcatc cagacggttt atcagccggg gagcttcacg
      481 ccactcatca gggtcgaaac cgccaccgga gagctggcga aaacgcagcg ccgcagcctg
      541 gcggatgccc ttcagcagtc cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg
      601 ctggtgcaga tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa
      661 agccgccgct ggctggcatc gtgcggcctg acggtggagc agatacaaaa ccagatggac
      721 ccggtgtaca cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg
      781 ctggcgcttg tcagcacgga aggggcaaca gaatggtgcg cagaatacga tgaatggggc
      841 aacctgctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccgggtcag
      901 cagtatgatg aggagtccgg cctctattac aaccgccacc gctattatga cccgctgcag
      961 gg
//
LOCUS       DAZGTJ010000299          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000299 DAZGTJ010000000
VERSION     DAZGTJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.305"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>959
                     /locus_tag="ACHK8E_005329"
     rRNA            <1..>959
                     /locus_tag="ACHK8E_005329"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          224 a          216 c          305 g          214 t
ORIGIN      
        1 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
       61 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
      121 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
      181 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
      241 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
      301 ttctaacgtt ggcccctgat cggggttgcg gacagtgtct ggtgggtagt ttgactgggg
      361 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
      421 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
      481 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
      541 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
      601 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
      661 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
      721 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
      781 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
      841 agagataagt gctgaaagca tctaagcacg aaacttgccc cgagatgagt tctccctgac
      901 tccttgagag tcctgaagga acgttgaaga cgacgacgtt gataggccgg gtgtgtaag
//
LOCUS       DAZGTJ010000300          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000300 DAZGTJ010000000
VERSION     DAZGTJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.306"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..661
                     /locus_tag="ACHK8E_005330"
     CDS             <1..661
                     /locus_tag="ACHK8E_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFP0347415.1"
                     /translation="FDAAYLLELDNYKYNFNKLAYPVFPENLPPVPERSDIIIKTDDP
                     VPCDGIWEPVKIHYDHKLLIIKDGISGFNNLGAYNYFIRGMNAPRQVYYDVLIESDTE
                     MVVPDDPIRYRDVHWRLVWEDTRYSDGIIPDESEYFLDDAPGKRITCPSGELCPHSGH
                     WATIAGGHQQFIDVQAGQLMPEATKYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGE
                     S"
     gene            739..>953
                     /locus_tag="ACHK8E_005331"
     CDS             739..>953
                     /locus_tag="ACHK8E_005331"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFP0347416.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          253 a          201 c          228 g          271 t
ORIGIN      
        1 tttcgatgct gcatatttat tagaattaga taattataaa tataatttta ataagttagc
       61 gtatccggtt ttcccagaaa atctcccccc tgttccagaa cgcagcgata ttattattaa
      121 aaccgatgac ccggttccct gtgacggcat ctgggaaccg gtaaagattc attacgacca
      181 taaactattg attattaaag atggtatcag cggatttaat aacctcggtg cttataatta
      241 ttttattcgt ggaatgaatg ctcctcgcca ggtgtattac gacgtattaa ttgaaagtga
      301 cacagaaatg gtcgtacccg atgacccaat ccgttaccgg gacgttcact ggcgtctggt
      361 ctgggaagac acgcgctaca gtgacggaat tattcccgat gagtcagaat actttctgga
      421 tgatgcacca ggcaaacgca ttacctgccc gagtggggag ctttgcccgc attcaggtca
      481 ttgggcgacc atagccggag gacatcagca gtttattgat gttcaggccg ggcagttgat
      541 gccagaagca actaaatatc agagcaatat gtatgcagat gaaatccatc ttcctgcgat
      601 atggaacctt ttacgccggg atgatgaggg gagtgtttat gtaaaaacag gggaaagtta
      661 aataactgat caacgctttt tgacggacag atctgttacc acctgatttg ctcaacaaaa
      721 gtgacattat ttgctctggt ggatggtcag cggtatttcg gtgagagcct gtctgttcaa
      781 caacctgccg ccgtgcccct gttcgatacc tggcctgatt cgcgtattgc atttgcgggg
      841 ccatgggtta tggagatgaa cagtataacg gatttcagag aaagactgtg tgagcttgaa
      901 gctgcgcttc cgtcagtttc ctggatgata tcttcttcct cgcttactga act
//
LOCUS       DAZGTJ010000301          940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000301 DAZGTJ010000000
VERSION     DAZGTJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.307"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>940
                     /locus_tag="ACHK8E_005332"
     CDS             <1..>940
                     /locus_tag="ACHK8E_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0347417.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQTE"
BASE COUNT          237 a          272 c          275 g          156 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aaggccaccg ggtgacggtg cattacaggt atgatgagaa aggccgcctg
      901 accggtgagc gccagacggt gcatcacccg cagacggaag
//
LOCUS       DAZGTJ010000302          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000302 DAZGTJ010000000
VERSION     DAZGTJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.308"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..801
                     /locus_tag="ACHK8E_005333"
     CDS             <1..801
                     /locus_tag="ACHK8E_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0347418.1"
                     /translation="TITVSYEFILCQHLITNTEFSYLALPENYNRLFLPNSKNKTNNG
                     LKTLNSEAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATAS
                     ILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLANLRKEFNKTV
                     RKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTNDFENLVLIE
                     NEPYHKVFTNMQSRIAKGILVGESKITPWAIPFGSIYPLMKNIMEPTK"
     gene            798..>928
                     /gene="yrhA"
                     /locus_tag="ACHK8E_005334"
     CDS             798..>928
                     /gene="yrhA"
                     /locus_tag="ACHK8E_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFP0347419.1"
                     /translation="MMTKTQINKLKKMMNDLDYPFEAPLKESFIESIIQIEFNSNST"
BASE COUNT          366 a          158 c          148 g          256 t
ORIGIN      
        1 acaataactg ttagttatga atttatcctc tgccaacatc ttatcactaa caccgagttc
       61 agctatttgg cactccctga aaattataac cgcttgtttt taccaaattc aaaaaacaaa
      121 acaaataatg gtttaaaaac gttaaacagc gaagctgttg gcaggctact tgctgctggt
      181 ggcgtataca atgggaacat tgaaggtttc agagatactg cggaaaaact gggtggagat
      241 gcaatcaaag gctatgatca aatactaaat gaaaaaacag cgggcatagc gatagcaaca
      301 gcatctattc ttttaacaaa gcgttctaat gttgatacat atacagaaat aaatagttac
      361 ttaggcaaac ttagaggtca acaaaaactt cttgatggta tagacataat agaaataata
      421 tacattaaga gaccttcaaa agacttagct aacttacgaa aggagtttaa taaaactgta
      481 agaaaaaatt ttcttatcaa acttgcaaaa acctccgaag catctggaag attcaacgcc
      541 gaagaccttt taagaatgag aaagggcaat gttcctctaa attataatgt tcaccataaa
      601 ctatctctag atgatggtgg tactaatgat ttcgaaaatt tagtattaat cgaaaacgaa
      661 ccatatcata aagtttttac taacatgcaa tcacgaatag ctaagggaat attagtaggt
      721 gaaagcaaaa tcactccctg ggccattcca tttggctcaa tttatcctct catgaaaaat
      781 attatggaac ccacaaaatg atgactaaaa cccaaataaa taaattaaaa aaaatgatga
      841 atgatttaga ctatccattt gaagcaccgc tcaaggaatc atttattgaa agtataatcc
      901 aaatagaatt taattctaat tcaactaa
//
LOCUS       DAZGTJ010000303          926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000303 DAZGTJ010000000
VERSION     DAZGTJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.309"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            258..707
                     /locus_tag="ACHK8E_005335"
     CDS             258..707
                     /locus_tag="ACHK8E_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347420.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQLS
                     DVKDAAEDVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
BASE COUNT          256 a          188 c          268 g          214 t
ORIGIN      
        1 attcgtcatg aaatcaacgg taatagtgtg tttctgtgac gggaataaaa aaatacagca
       61 ccgccgatga cccggtgctg aaaacaggtt aaatgtagtg ggcacatttc cggtaatatc
      121 aggaacagca tgttatggtt ataaaagcat ataccgggag tgtgccctga cactgtgatg
      181 ttctggttgt cttttgacca ttttccgggg acgaaatata agctgttctg cgttaacact
      241 taataatgag agattgtatg ctgaaaaaaa caattctggc gttatgtatt tccgcaccat
      301 ttctggttgc gggctgtgat gatgcgacaa aggagaatgt aagtaagaag gcggcagata
      361 taaaggaagc tgcgcagact caactcagtg atgtgaagga tgccgcggag gacgtgaaaa
      421 tgcaggctgc cggaaaagtg gcagaggttt ctgaagcagc tcagtccggg ctcagcgagg
      481 tgaaggatgc cgtggaggaa gtgaaaacac aggcagccgg aaagatggca gaggtctctg
      541 aagcggctca gtccgggctc agcgaggtga aggatgccgt ggaggaagta aaaacacagg
      601 cagccggaaa ggtggcagag gtctctgaag cgactcagtc cggtctcagt gatgtgaagg
      661 ataccgctcc tgaaaagggg gctgaaatat ccgtaacgga ggagtaaaag agcgccgccg
      721 gtgcagtttt tttcagcacc cggtgacacg gaattttata tacggggcat gtctgtctct
      781 ttaataaatc agcatattta ccggatatcc gatgccctgt acctgcgctg tcattatggc
      841 tctcaaacag acaatgcttt gtctggagca caggaactct gtcatacctg ccggcacaat
      901 gcttctgatt gccgggcatc tgcgcc
//
LOCUS       DAZGTJ010000304          925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000304 DAZGTJ010000000
VERSION     DAZGTJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.310"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>925)
                     /locus_tag="ACHK8E_005336"
     CDS             complement(<1..>925)
                     /locus_tag="ACHK8E_005336"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP0347421.1"
                     /translation="FNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQL
                     AMHGQTHVNKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLH
                     NINYDKFDIKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGV
                     LAPKSKADFAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVIS
                     LAPNLFFGTTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFA
                     SKEDVAHLAKSVAFETVVANDYNLSVSSYVEA"
BASE COUNT          236 a          217 c          228 g          244 t
ORIGIN      
        1 cgcttccaca tagctgctca ccgacaggtt atagtcatta gcgacaacgg tctcaaacgc
       61 gactgatttc gccagatggg caacatcttc cttgctggca aatacctgca taatctgttc
      121 gatatgagca tcggtcagaa tgttgttgtt agcctctttt ttgaacagtt cgctggcatc
      181 aataaactga actctggtat ccgttttatg tttagacagc accagtatgt ttacggcaat
      241 ggtggtgcca aagaacaggt tcggtgccag tgaaatcacg gtttcgacat agttattgtc
      301 gaccagatac tgacggattt tctgctctgc gccgccacgg taaaaaatac ccgggaagca
      361 gacaatcgca gcgcgacctc tggccgaaag atagttcagc gcatgtaata caaacgcaaa
      421 gtcagctttg gatttggggg ccagaacgcc agccggggca aaacgctcat cgttaatcag
      481 tgtcgggtca tcgctgccag tccatttcac cgaatacggc gggttagaaa cgatggcatc
      541 aaacggtttt tcatctctga agtgcggctc agtcagcgta ttgcccagct tgatatcaaa
      601 cttgtcgtag ttgatgttgt gcaaaaacat gttcatacgc gccaggttat aggtcgtatg
      661 gttgatttcc tgaccaaaaa agccttcttc gatgatatgg tcatcaaact gctttttcgc
      721 ctgcaacaac agcgaaccgg aacccgctgc cgggtcgtag attttgttaa cgtgggtctg
      781 cccgtgcata gccagttgtg caatcagctt agagacgtgc tgtggtgtaa agaactcgcc
      841 gcctgactta ccggcatttg cggcatagtt agaaataagg aattcatagg catcgccaaa
      901 caggtcaatc tgatgtttgt tgaag
//
LOCUS       DAZGTJ010000305          920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000305 DAZGTJ010000000
VERSION     DAZGTJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.311"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>920)
                     /gene="tssK"
                     /locus_tag="ACHK8E_005337"
     CDS             complement(<1..>920)
                     /gene="tssK"
                     /locus_tag="ACHK8E_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0347422.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYV"
BASE COUNT          238 a          270 c          239 g          173 t
ORIGIN      
        1 acatagaaca tacacactcc ctgctccagc atttcatgtg cagcgggact ttccatatca
       61 agtgcgaagt actggttttc catacgtaca ggcaacgcag ccggaaccct gctcaccggg
      121 atcaaaggga tccccctgag tgccgcgcca tagatctcac tgacattatc aggtgaaccc
      181 gccagacaga gtgccggaaa tttctcagca acctgccagg ccggagtatc cgagcgcaca
      241 gacagccaga ggtccgcctc ttcgcgcagg cggatatcgt gaaggctggc cttccaggtc
      301 tgtccatccg ggcgggacat ttccagagca atgacccggg atggcagaca ggcctccagc
      361 agacccgtta tcaggtcgaa cagcggaaga aacgcaccgg ccggatcctt gtggtcataa
      421 cccggtatgg cgtcaggatc atgatccagg gaaaaagtca gcatactgcc tgccagacga
      481 gcgagctccg cccacacctg ttcaggtgga cgggaaggaa aacgttcgta ttccgtcagt
      541 acactggcgt gggagttcag cgcattcagc aaccagaaaa gggaaacatc agcgacagca
      601 aaatcggcga gtctttcgtt actttcacga cgcatcgcca tcagacgctg gcgtcgggag
      661 cgtaactgac gattgagcaa caccagacgt tcgcgcagaa ccggacttgc cccaaataac
      721 gccaccggag gaataaaaga cggatcctgt cgccacgcat tctgaccatc tctgatcaat
      781 cttgctaccg gacagacttt ccatgaatca ttgttttcat gggcaaaccg gatggtgaaa
      841 ttaaaccggg caacggctat gggctcttct tcagagccaa aagcatcctg cagcgtgacc
      901 cactcttccc gatagcgaac
//
LOCUS       DAZGTJ010000306          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000306 DAZGTJ010000000
VERSION     DAZGTJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.312"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..499
                     /locus_tag="ACHK8E_005338"
     rRNA            <1..499
                     /locus_tag="ACHK8E_005338"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            568..644
                     /locus_tag="ACHK8E_005339"
     tRNA            568..644
                     /locus_tag="ACHK8E_005339"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:602..604,aa:Ile,seq:gat)
     gene            687..762
                     /locus_tag="ACHK8E_005340"
     tRNA            687..762
                     /locus_tag="ACHK8E_005340"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:720..722,aa:Ala,seq:tgc)
BASE COUNT          243 a          211 c          248 g          216 t
ORIGIN      
        1 acaggtgctg catggctgtc gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac
       61 gagcgcaacc cttatccttt gttgccagcg gtccggccgg gaactcaaag gagactgcca
      121 gtgataaact ggaggaaggt ggggatgacg tcaagtcatc atggccctta cgaccagggc
      181 tacacacgtg ctacaatggc gcatacaaag agaagcgacc tcgcgagagc aagcggacct
      241 cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg
      301 ctagtaatcg tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc
      361 cgtcacacca tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct
      421 taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg
      481 cggttggatc acctccttac cttaaagaag cgtactttgc agtgctcaca cagattgtct
      541 gatgaaaatg agcagtaaaa cctctacagg cttgtagctc aggtggttag agcgcacccc
      601 tgataagggt gaggtcggtg gttcaagtcc actcaggcct accaaatttg cacggcaaat
      661 ttgaagaggt tttaactaca tgttatgggg ctatagctca gctgggagag cgcctgcttt
      721 gcacgcagga ggtctgcggt tcgatcccgc atagctccac catctctgta gtgattaaat
      781 aaaaaatact tcagagtgta cctgcaaagg ttcactgcga agttttgctc tttaaaaatc
      841 tggatcaagc tgaaaattga aacactgaac aacgaaagtt gttcgtgagt ctctcaaatt
      901 ttcgcaacac gatgatga
//
LOCUS       DAZGTJ010000307          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000307 DAZGTJ010000000
VERSION     DAZGTJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.313"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..398)
                     /gene="ybfB"
                     /locus_tag="ACHK8E_005341"
     CDS             complement(72..398)
                     /gene="ybfB"
                     /locus_tag="ACHK8E_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFP0347423.1"
                     /translation="MKCIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(398..>916)
                     /locus_tag="ACHK8E_005342"
     CDS             complement(398..>916)
                     /locus_tag="ACHK8E_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0347424.1"
                     /translation="KGGWNFYQYPLNPITNIDPLGLETLKCIKPLHSMGGTGERSGPD
                     IWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVET
                     DNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNI
                     GRFIRFVFTGVM"
BASE COUNT          297 a          207 c          136 g          276 t
ORIGIN      
        1 cctgccgggt ctgtggcata cgggatatca atgcccaggt tacatataat aacaagagca
       61 attaacttaa tttatttgtt tataatgcgc ttacttaata caaaccattg aatataccct
      121 cctgacagaa aataaagcca tattattatc gccataatat acttactatc aatccttacg
      181 ccaataatgt ctgaaacagt ccctataaat attagagcaa taaagaatac tatgcctgtt
      241 ggaaaacata ttaccatcat tccataagac atgagactaa ttaactcgct cacatcacga
      301 gtggagtcta atagtgaaag tctatgcatg gaaaagaata gtattaaaag cgataaagcc
      361 agccatatag ctctgaataa aaaaataata catttcatta catcactccg gtaaatacaa
      421 atctaataaa acgtccaatg ttatttccgg agcacttcat cttacatgtt tctaaagaat
      481 cattagacca gtcttggcag tttttaaata gccctaaatt tataggtgtg aatatatcag
      541 gcaatacaga ataacgcggc cttacctctt taaacttccc ttttaaacag ttctccacac
      601 aactattatc ggtttcaacg aggtcacaac ggccagcagc ttcttttgta tcattacttg
      661 ctttacctgg cccccataga ccatctcctt ttgattctcc ccgttggtct tggccaccac
      721 aagtatagtc ccctttacca tctgggacac aaagatattg atgatagaac ggattccccc
      781 atatatctgg accgcttctt tcaccagttc cgcccattga atgcagtggc ttaatgcatt
      841 ttagtgtttc taatcctaaa ggatctatat tagtgatcgg attcaacgga tactgataaa
      901 aattccatcc cccctt
//
LOCUS       DAZGTJ010000308          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000308 DAZGTJ010000000
VERSION     DAZGTJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.314"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>896)
                     /locus_tag="ACHK8E_005343"
     CDS             complement(<1..>896)
                     /locus_tag="ACHK8E_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP0347425.1"
                     /translation="VDSGDKNIEEQSESELAPEQKRTGIAKMLMTTPVLTLIFDRVRY
                     DEIELQPDFQRKDRIWPDDKKSKLIESILMKLPLPGFYFGEKPNGNWVVIDGLQRTTT
                     ICDYMSGHFSLKGLSILEHLNGKSFKDLTRTEQRDIREYQITAYQIELNDDSSELVVE
                     LFHRINTYGVKLSSQEIRSALNKGNSVTFLRYLASLETFKKATQFKVKPDRQKDMELC
                     LSALAFMVLGYNNYGQHSYDHFLCSAMQKLNNYPLSIINKEEIDAGTALISPSSEVFL
                     TLYSKYNQALILANEVFGEIAFS"
BASE COUNT          279 a          169 c          136 g          312 t
ORIGIN      
        1 gaaaaagcta tttcaccaaa aacttcattt gctaagatta aagcctgatt atattttgaa
       61 tataaagtta aaaagacttc acttgaagga gaaataagag ctgttccagc atcaatctct
      121 tctttattaa tgatgcttaa tggataatta tttagcttct gcattgcact acataaaaaa
      181 tgatcatacg aatgttggcc atagttatta taaccaagta ccataaaagc aagagcggac
      241 aaacagagct ccatatcttt ttgcctgtcc ggctttactt taaattgcgt cgcttttttg
      301 aacgtttcta atgaagctaa ataacgtaaa aaggttacgc tattgccttt attaagtgca
      361 gatcttattt cttgactact taactttaca ccataagtgt ttattctatg gaatagttct
      421 acaaccaatt cagaactatc atcatttagt tctatttggt aagccgtaat ttgatactct
      481 ctaatgtctc gttgctcggt tcgagtaaga tctttaaaac tctttccatt taagtgctct
      541 aaaatagaga gccctttcaa tgagaaatga ccactcatat aatcacaaat tgtagtcgtt
      601 ctttgaaggc catcaataac tacccagtta ccgtttggct tttcaccaaa gtaaaacccc
      661 ggtaatggaa gcttcattaa tattgattca ataagttttg actttttgtc gtcaggccaa
      721 attcggtctt ttctctgaaa gtcaggttgt aactcaattt catcatatct tacacgatca
      781 aaaattagtg ttaaaactgg tgtagtcata agcatttttg caatgcccgt acgtttttgc
      841 tcaggtgcta attctgattc cgattgttcc tcaatattct tatcacctga atcaac
//
LOCUS       DAZGTJ010000309          889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000309 DAZGTJ010000000
VERSION     DAZGTJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.315"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            357..584
                     /locus_tag="ACHK8E_005344"
     CDS             357..584
                     /locus_tag="ACHK8E_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0347426.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            653..>889
                     /locus_tag="ACHK8E_005345"
     CDS             653..>889
                     /locus_tag="ACHK8E_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347427.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGT"
BASE COUNT          200 a          276 c          225 g          188 t
ORIGIN      
        1 actgacgccg cgctctgtga actgttaccg gactgagtga tcatcatgca cacacaaaaa
       61 aaccgcttgc cagcgttcgc ctggtcgggt tacagttttc ccgcacctca taaaacgggt
      121 gccgggattg caacccccgc tgactacaca agcgcacaac cgcgccagcg gtttttttgt
      181 gcgtaccgca tcgtcacatc tttttcgcgt cagaattatg gtggggcgta cagggccgac
      241 ttcggtcggg ccggggtctt gtgtagccgg tggttgcaac cctgtacgtc tcaccaccca
      301 gaattgcaac ctctggatgg tgagttttca aaacttacta cacaagaggc cacaccatgg
      361 caaaccgcaa gcaacagcgc gcccgcgctg agcgtcttca catccgcacc gaaatcaacc
      421 gcagactctg gcgcgcatca cgcgtcgcgc aaatcatgta catcaacatg ctgcatgaac
      481 acgcacacgc gctgtcagac cgttactgtg ccgccgtctt cagctatctg gcggatgacc
      541 tggcagagct gcaggacctt atcaaccagc aacaccacca ctaaacaaca tcctgaagcc
      601 atcccgcacc cgtgcgcggg aggcttccgc acgtctgtta ccggaggttc tcatgaagaa
      661 cgttaaggga ctttcagata ttcaggactt ttttaactgg atgtgggcag tggatccccg
      721 gctggcttcc cgcctgaacg actggcacga ctattaccgc cgcgcctgta aacaggccgc
      781 cagacgcgtc accacatacg gggatttctc cttcaccgtt gacgaccgtt accgcgtgga
      841 cgtgaaaggc gatggcgatg gctttggttt atttcacatc aacggcacc
//
LOCUS       DAZGTJ010000310          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000310 DAZGTJ010000000
VERSION     DAZGTJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.316"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            12..812
                     /locus_tag="ACHK8E_005346"
     CDS             12..812
                     /locus_tag="ACHK8E_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347428.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          276 a          165 c          205 g          236 t
ORIGIN      
        1 agtgtccgat tatgaattca aaaaaggttt gttgcatatg tgtattattc tcgctgcttg
       61 caggatgtgc ctctcaatct tctattgatg aaaaaaagaa aaaagctcaa gttacacaaa
      121 gtaatattaa taaaaacatt cccccgcaac tgacagacaa agatttattc ggtaatgaaa
      181 ccactctggc tgtatccgag gaggatattc aggctgcgct tgaaggagat gagtttcgtg
      241 tgcctctcaa ttctcccgta atacttgtcc agtctggcag tcgcgcaccg gaaaccatta
      301 tgcaggagga gatgagaaaa tattatactg tttccacatt ctctggtatc ccggacaggc
      361 aaaagcctct gacttgtaac aaaaataaag ataaaaatga gaacgaggat gttgccagtg
      421 ctgaaaatat gaactggatg caggcattgc gttttgtggc tgcgaaaggg catcagaaag
      481 cgattattgt ttaccatgat acgttgcaga caggaaaata tgactctgcg ctgaaatcaa
      541 cagtatggtc cgactataaa aatgaaaaac tcactgacgc tatctccctg cgctacctgg
      601 tacgtttcac gctggtggat gtggcaacag gtgagtgggc tacctggtcg ccggtgaatt
      661 atgaatcgag agtaattttt ccgccggctg gaataacaaa aatgagcaat aaagaactat
      721 caaatgacca tgttacggaa gtgcaactgt ttaatttgaa acaaaaaaca tatgtatcga
      781 tggtgaaaga cttggtgagt cgattccagt aatcgttatt gttatgctgc cgcctgtagt
      841 ttagggcggg cagcaaacag gagctgagta tttctataat gt
//
LOCUS       DAZGTJ010000311          879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000311 DAZGTJ010000000
VERSION     DAZGTJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.317"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..402
                     /locus_tag="ACHK8E_005347"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHK8E_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            394..729
                     /locus_tag="ACHK8E_005348"
     CDS             394..729
                     /locus_tag="ACHK8E_005348"
                     /inference="COORDINATES: protein motif:HMM:NF026979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain fold toxin domain-containing protein"
                     /protein_id="HFP0347429.1"
                     /translation="MAKTRDLATTISDKFKQLFQCDKFAAALKSKMQQAGVNGEHLIL
                     ETKNKQGAFGNIWSDRIGKNIATNGHHEAIKVGDTVFDNMNPSGIKYEDWLNDIYSPG
                     EYTITPSRF"
BASE COUNT          275 a          172 c          221 g          211 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgataca gtttttgata atatgaatcc ttctggtata
      661 aagtatgaag attggctgaa tgatatatac tctcctggcg aatacaccat tactccatca
      721 aggttttaat tatgaatgat attgttaaac tgattgatat gatagaaaaa agaccagctt
      781 tatatttaag caggcatacc atatcggcgt taaaagcttt cctggatgga tggcaaatga
      841 gagagccaaa agaaatcgtc aatattgaga tattaataa
//
LOCUS       DAZGTJ010000312          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000312 DAZGTJ010000000
VERSION     DAZGTJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.318"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>877
                     /locus_tag="ACHK8E_005349"
     CDS             <1..>877
                     /locus_tag="ACHK8E_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0347430.1"
                     /translation="VPNNEAIVSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFL
                     TGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDV
                     AIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTC
                     LFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLV
                     PNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGA
                     AAGVFGNATGGRRGA"
BASE COUNT          182 a          194 c          235 g          266 t
ORIGIN      
        1 tagtgccgaa caatgaagcc attgtttccg tagcgcagaa aagcttcggg aaagaaatgg
       61 cgatgattat gttctttgcg atggttatca acattatgat tgcccgtttc acaccgtgga
      121 aatttatctt cctgaccggt catcacacgt tgtttatgtc gatgatggta gcggtaattc
      181 tggcaacagc aggcatgacc ggcattacgc ttatagccgt tggctctttg gttgttggcg
      241 tggcaatggt ctttttcccg gctattgcgc atccgtacat gaagaaagtg accggttctg
      301 atgacgttgc catcggccac ttctcaacat tatcttatgt attggccggt ttcatcggca
      361 gcaagtttgg taataaagag cactcgactg aagacatgaa tgtgccgaaa agtctgctgt
      421 tcttgcgcga cacgccggtt gctatctctt ttaccatgag cataattttc ctggtgacgt
      481 gcctgtttgc gggtgcagat gcggtgaaag agcttagcgg tggtaaaaac tggttcatgt
      541 tctctatcat gcaatccatt accttcgcag ctggcgtgta catcattctg cagggtgtgc
      601 gcatggtgat tgcggagatt gtcccggcat tcaaaggtat ttcagacaag ctggttccga
      661 atgccagacc tgctctcgat tgcccggtcg tcttccctta cgcacctaac gctgtactgg
      721 ttggtttcct gagtagcttt gctgcaggtt taatcggtat gttcacgttg tatctgctga
      781 acatgatcgt gattattcct ggtgtggtgc cgcacttctt cgtaggtgca gctgcagggg
      841 tattcggtaa tgcaacgggt ggacgtcgtg gtgcgat
//
LOCUS       DAZGTJ010000313          874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000313 DAZGTJ010000000
VERSION     DAZGTJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.319"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..569)
                     /gene="fimE"
                     /locus_tag="ACHK8E_005350"
     CDS             complement(<1..569)
                     /gene="fimE"
                     /locus_tag="ACHK8E_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP0347431.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLI"
BASE COUNT          283 a          190 c          169 g          232 t
ORIGIN      
        1 tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact ggcggtataa
       61 cgtacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg ggtatcggca
      121 ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg agtctgcgtt
      181 acggttccag cttcaatacc tgcatcgcga ataatgcgat aggcctgctg gcgagaaagc
      241 cgactcccgc ggcgagaaat aaatatagcg tcggtccggt cagcgccttt ccagttagca
      301 cgttcctggg tccagcgttc cacggcttca cgctcatcaa aacgtaacgg gtgaacggta
      361 gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc aagatcctga
      421 taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa cagaataaga
      481 caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc ctgaacttct
      541 ttaccggtaa gataacgacg tttactcaca atagtagtac tcctaactga gatatatttg
      601 aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata aaaagataac
      661 cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa aaataaaata
      721 tcactgatgt gacaacatct attttcattg atttgattca atataacaat agattgcaga
      781 agattaactt ccagttgctt aaaaaataaa gaaaaaagtg tggaaacagg agaatttaag
      841 tgatgtgtaa atttatgaat gattttgttt attt
//
LOCUS       DAZGTJ010000314          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000314 DAZGTJ010000000
VERSION     DAZGTJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.320"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>872
                     /locus_tag="ACHK8E_005351"
     CDS             <1..>872
                     /locus_tag="ACHK8E_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347432.1"
                     /translation="FSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDHST
                     SFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGVAT
                     VQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQMSP
                     GAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAGLE
                     KKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNADY
                     SELAQSPKFQQTFLS"
BASE COUNT          220 a          194 c          270 g          188 t
ORIGIN      
        1 ttttcagatg agcagcgccc tgaagcgcaa ttgggtaacc agaaccgcag cagcctgaac
       61 attaagcagc cgggagaaaa ttactggcag gattttttca gcaacccgca gaatgccatt
      121 gatcacagca catcgttcag cctcggtgat gtattgccaa caatgggtaa aggtgttgcc
      181 cagtccgtcc ggggaacagg ggaaatggcc cgtggactcg gtgatgcgat gattcagagc
      241 ccggtaaaaa caggggcgcg tattttaaat gagttcagcc gtatggggct accgggtgtc
      301 gcaactgttc aggatatttt tgccggtggc agcagggggg ctgatgaggt catcgatacc
      361 ctgcctgatg gaaaaaacgt ggtcactgat actgtcggta aaggtctgaa agcaaccggt
      421 aaggctgtca gtgatggtgc caaagcctcg gatgaatggc tgacagaaca gatgtcgcca
      481 ggtgctgttc gtgcgctgaa tacgccgata acagaaggct atgatgattc tgcggtcttg
      541 ctggcgaagg gtgtaaacct gattggtgcg cttgtacctg atatggttgc tggcggtgtg
      601 gctagaaagg tgggtgatgt cacactgcga aaaatgctga ccgccgggct ggagaaaaaa
      661 tacatcgcgg cagggatgca gccggaaaga gccacggcac tggcagcaga agctgtcgat
      721 aaaaaaatgc cggatttatt ccaggcgggc ctgatcaccc attccacagc cagtgcacag
      781 gggcagagtg caatggcggc agcagatgct gttcttaatg cggattactc tgagctggcg
      841 cagtcaccga aatttcagca gacgtttttg tc
//
LOCUS       DAZGTJ010000315          857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000315 DAZGTJ010000000
VERSION     DAZGTJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.321"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..799
                     /locus_tag="ACHK8E_005352"
     CDS             83..799
                     /locus_tag="ACHK8E_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0347433.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          286 a          146 c          176 g          249 t
ORIGIN      
        1 cctgttatga tataatatgc cgaattaaca catgttaacg atttaccagt aatgtaaata
       61 aattttcgag gagatcattc cagtgggacg taaatgggcc aatattgttg ctaaaaaaac
      121 ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc ggtgtagaaa tctatgctgc
      181 tgccaaacaa ggtgaacccg atccagaatt aaacacatct ttaaaattcg ttattgaacg
      241 tgcaaagcag gcacaagttc caaagcacgt tattgataaa gcaattgata aagccaaagg
      301 cggcggagat gaaacgttcg tacagggacg ttatgaaggc tttggtccta atggctcaat
      361 gattattgct gagacattga cttcaaatgt taaccgtacg attgctaacg ttcgcacaat
      421 tttcaataaa aaaggcggca atatcggagc ggcaggttct gtcagctata tgtttgacaa
      481 tacgggtgtg attgtattta aaggaacaga ccctgaccat atttttgaaa ttttacttga
      541 agctgaagtt gatgttcgtg atgtgactga agaagaaggt aacattgtta tttatactga
      601 acctaccgac cttcataaag gaatcgcggc tctaaaagca gctggaatca ctgagttctc
      661 aacaacagaa ttagaaatga ttgctcaatc tgaagttgag ctttccccag aagatttaga
      721 aatctttgaa gggcttgttg atgcccttga agatgacgac gatgtacaaa aagtttatca
      781 taacgtcgca aatctctaat gatattttaa agaaatctgt ctttatggca gatttcttta
      841 atctcatata attctta
//
LOCUS       DAZGTJ010000316          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000316 DAZGTJ010000000
VERSION     DAZGTJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.322"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..406)
                     /locus_tag="ACHK8E_005353"
     CDS             complement(<1..406)
                     /locus_tag="ACHK8E_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFP0347434.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVG"
     gene            complement(399..>848)
                     /locus_tag="ACHK8E_005354"
     CDS             complement(399..>848)
                     /locus_tag="ACHK8E_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFP0347435.1"
                     /translation="AKTAQVQGNFNNMIMLRVRENRTAELLTTQLSQVEIYTKTLVSG
                     HQDTADVTTGQDFTSSTQDRVGTVKVPLLEPADIVTLPKGQAFALLEGGQLWKIRMPL
                     PATDADDALMPESIAKIAEAMRRNYHTGEGWWNETPSVNMPEGEQNG"
BASE COUNT          183 a          272 c          202 g          191 t
ORIGIN      
        1 gccccacatc ctcactgtac tgtgacacac tgttacccac cacggcaccg gcattttgct
       61 gcttcagcca cgacagaaaa cctgtttcca caaacagcca ctgatacgcc gttgctatcc
      121 attctgtcac cgttaccgat ggtgtcgaca gcagcagact gcgggtgaat tccgaagaca
      181 gccaccccag ttctgtcttc atcactgcct cactgtgtgc tgccccctgc tccggccaga
      241 aaaaagcaat cccgatatac tcaaccagca ggctcagcag cagggaggaa aacaggatcc
      301 cgaccagcat ccacggccag ccccacaaca gattccagag cagtccgtgc tgacgtggcg
      361 gagagggttg tgctgctctg ttcacctcac gacggggttc agccattctg ctctccttcc
      421 ggcatgttca cggagggagt ctcattccac catccctctc cggtgtggta attacgccgc
      481 atggcctctg caatttttgc aatactctcc ggcatcagcg cgtcatctgc atcagttgca
      541 ggcaggggca tacggatttt ccacaactgt cccccttcca gcagagcaaa ggcctgtcct
      601 ttcggcaggg tcacgatatc tgccggctcc agcaaaggca ccttcacagt accaacacga
      661 tcctgggtac tggatgtgaa gtcctggccg gtcgtgacat ctgcggtatc ctgatgaccg
      721 gaaaccagcg ttttggtata aatttccacc tgggacaact gggtcgtcag taactcggcc
      781 gtccggttct ccctgacccg cagcataatc atgttattaa agttaccctg cacctgggcc
      841 gttttggc
//
LOCUS       DAZGTJ010000317          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000317 DAZGTJ010000000
VERSION     DAZGTJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.323"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHK8E_005355"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHK8E_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0347436.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>848)
                     /locus_tag="ACHK8E_005356"
     CDS             complement(369..>848)
                     /locus_tag="ACHK8E_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0347437.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          209 a          238 c          215 g          186 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaagat atagggaaga cgggcattaa tacggctgtt cgccgttgca
      721 tccggcgtat cgtacagtgc cggtttctgg gtggagttag cggagaagaa gcaggcataa
      781 tcccggttct tgtagtaaga gagcggaata aagcccagat tagcgaattc aaactcccgc
      841 gtttcagg
//
LOCUS       DAZGTJ010000318          844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000318 DAZGTJ010000000
VERSION     DAZGTJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.324"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..246
                     /locus_tag="ACHK8E_005357"
     tRNA            171..246
                     /locus_tag="ACHK8E_005357"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:204..206,aa:Ile,seq:cat)
BASE COUNT          245 a          162 c          185 g          252 t
ORIGIN      
        1 acatgcttgt tgactgcgag tatcgacttt gcgaatggaa gcggtgtcgg gatttatgtc
       61 gcatgtattg acgacccagg atggatgaga acgcataaaa atctgttatt tacatcataa
      121 gatcatcatg tttattgcgt ggtttataag tattgttatt gcatacttaa ggccctttag
      181 ttcagtggtt agagcaggcg actcataatc gcccggtcgc tggttcaagt ccagcagggg
      241 ccaccaaatc atcattcatg tatcaagaag agttaagggt acaaggggta ctcgatggcg
      301 gtctatattt catgactaac gccatttaaa ggaagaagtg cggtgggtgg tttcgaatga
      361 gggctggtgt tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc
      421 aaaagtacct ggaaaaaatg cccgctatgc caagcgggca aattgcttac atgtggaatt
      481 aagtaattaa cgataaagcc agagtattat catttatcat gcctcttcat ttaatcaggt
      541 taattactca aatcactgaa ctattctctg ttcgctcact gatgataagt gaagtgtcac
      601 aaccacgatt tgtatgtgga ccaatgccgt agagtaagga tgaaatgtct ctgccacgcc
      661 ttaaatatct actactgacc agaacacccg ccgcaggcag atgagtctta tgagcgttac
      721 aggagtctgg ctttgctcat taattaacgc tatttagata taaatttcta acactcgaaa
      781 aacgaattcc actaataaat cataacggtt ggtttttatc atacagcgaa ataacttttt
      841 gttt
//
LOCUS       DAZGTJ010000319          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000319 DAZGTJ010000000
VERSION     DAZGTJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.325"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..747
                     /gene="ydeO"
                     /locus_tag="ACHK8E_005358"
     CDS             <1..747
                     /gene="ydeO"
                     /locus_tag="ACHK8E_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP0347438.1"
                     /translation="SVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFK
                     EIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFS
                     ELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISES
                     LLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHF
                     GNSPKRVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          256 a          166 c          168 g          252 t
ORIGIN      
        1 tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc
       61 tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag
      121 cggcatttca aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact
      181 aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg
      241 actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat
      301 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccacatgcaa aggtttcatt
      361 acactattaa ctaacggtgt gctatccgtt tccgggaaag tgagaaatat tgtcaacatg
      421 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat tagtgaaagc
      481 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct tttagatgcc
      541 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      601 caatgtggtt atgcgagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      661 agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaacacggat
      721 aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgtt
      781 acgtagaaat taaaatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatatt
      841 at
//
LOCUS       DAZGTJ010000320          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000320 DAZGTJ010000000
VERSION     DAZGTJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.326"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..751
                     /locus_tag="ACHK8E_005359"
     CDS             <1..751
                     /locus_tag="ACHK8E_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HFP0347439.1"
                     /translation="ILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFN
                     EHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITE
                     DLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFG
                     PVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSL
                     LGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT          148 a          191 c          277 g          217 t
ORIGIN      
        1 catcctggcg cgttttatgg cggcagattc tcaccacagc attgtccatg cgcgtcgtat
       61 cagtatgacc tggatgatcc tctgcctggc aggggcggtg gctgtcggct tctttggtat
      121 cgcttacttt aacgagcacc cggcggtagc aggtgcggta aaccagaacg ctgaacgcgt
      181 gtttattgaa ctggcgcaaa ttctgtttaa cccgtggatt gccgggattt tgctgtcggc
      241 gattctggcg gcggtaatgt caacgttaag ttgccagctg ctggtatgtt ccagtgcgat
      301 taccgaagat ttatacaaag cgtttctgcg taaacaggcc agccagaaag agctggtgtg
      361 ggtagggcga gtgatggtgc tggtggtggc gctggtggcg attgcgctgg cggcaaaccc
      421 ggaaaaccgc gtgctgggct tagtgagcta cgcgtgggca ggctttggcg cagcgtttgg
      481 tccggtggtg ctgttctcgg tgatgtggtc acgcatgacg cgcaacggtg cgctggcggg
      541 gatgatcatc ggtgcgctga cggttatcgt ctggaaacag ttcggctggc tgggtctgta
      601 cgaaattatt ccgggcttta tcttcggcag tatcgggatt gtagtgttta gtttgctggg
      661 taaagcgccg tcagcggcga tgcaaaaacg ctttgccgag gccgatgcgc actatcattc
      721 ggctccgccg tcacggttgc aggaagggta agggacttag cctgcggcgg ttttgtttgg
      781 cttcagcagc gggttgcgct cccttaatgt gcctcgccat ataaattgaa tgg
//
LOCUS       DAZGTJ010000321          820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000321 DAZGTJ010000000
VERSION     DAZGTJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.327"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          231 a          198 c          165 g          226 t
ORIGIN      
        1 gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact cacaatccct
       61 tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg ttcgtatacc
      121 ctgttcgatg gattattatt gatccatatg atcgcttcac gtaaacacga ttctggctcc
      181 acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg tcacagacgg
      241 tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa actctctatg
      301 cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca agcgaatgac
      361 tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat acactcagac
      421 tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcctctttat cttcttatgg
      481 cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc cccggtaaga
      541 aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg catattttca
      601 tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt aaagaatgct
      661 ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc ctctggagaa
      721 aagtgctact tcttgtcgat tcagactagt tcaggaaatt atacgaaaat attacctgaa
      781 tgagctaaat gttatttacc ttcctttctg ttcattgtaa
//
LOCUS       DAZGTJ010000322          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000322 DAZGTJ010000000
VERSION     DAZGTJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.328"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>818
                     /locus_tag="ACHK8E_005360"
     CDS             <1..>818
                     /locus_tag="ACHK8E_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFP0347440.1"
                     /translation="RDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLRGYVK
                     PHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRWIGKN
                     NHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDRDILY
                     RMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEKLRTP
                     PEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAVG"
BASE COUNT          180 a          198 c          286 g          154 t
ORIGIN      
        1 cgggatacgg tggaggatgc gctgcaggac agttttgatg aatatgactc acatccctgg
       61 gtggtgcagt tcttctgtca ggatgaaaac gacgtggaca cgtatgttga ccagctcagg
      121 gggtacgtga aacctcatgc agaaggctcc tcatttacgg aggcctggct gagggaaatg
      181 gagcgccatc tgaaggggat tgcccgaccg gaaggtctgt tcagggatac gctggtcaca
      241 ggtcagccat ggcgtggtca gcagcggcgc acgaggatgg tgatttaccg gtggatcggg
      301 aaaaacaacc acgatccgat gccgccggtg gcaatgctga accaggtctg ttcgcgggtc
      361 gtgggggcgc tgggtggcgc aggcgtccgc tgtacacgga tgaacgggca gcaggtgcat
      421 ggctggctgc tgaggctgtt caatccccgg ccggaatggg tggacaggga tattctgtac
      481 cgtatggcat cccgtgcaga accgcaggag acaccggagg gaatgatgcc ggtgatgacc
      541 gattttgcag aaagcctctg gttcacccca ccggtatctg atccggaaaa cggcgtgtgg
      601 tggctggacg ggcttccgca tgccgctgtt gtggtggaga aactgcgcac accaccggag
      661 ccgggaacca tcacagggga acaggcccgg ggcgaaaaga cagtgaatgc cctgatggac
      721 acctttccgg agggaacggt gctgtgtatg acaattgtgg tccagccaca ggacacactg
      781 gaagagcgct tcacgaggtt gtcgaaaaat gcggtcgg
//
LOCUS       DAZGTJ010000323          817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000323 DAZGTJ010000000
VERSION     DAZGTJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.329"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..209
                     /locus_tag="ACHK8E_005361"
     CDS             <1..209
                     /locus_tag="ACHK8E_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP0347441.1"
                     /translation="KNNPGKNLDKIHPGDILYYQKASMKVIITGWKPITIKNVAMNYN
                     GGGDPKYAIKLQFVYTLLTKNRVL"
     gene            212..661
                     /locus_tag="ACHK8E_005362"
     CDS             212..661
                     /locus_tag="ACHK8E_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFP0347442.1"
                     /translation="MGKITIILLLTFSCTKCLAFDISMSKECSGVDELEFVDCVKNSF
                     IMSTITLKKAEVNIKEKIKKWNNDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFA
                     LTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT          313 a          133 c          132 g          239 t
ORIGIN      
        1 cgaaaaacaa ccctggaaaa aatctcgata aaatacaccc gggagatatt ttatattatc
       61 aaaaagcatc catgaaagta ataattactg gatggaaacc tatcacaata aaaaatgtag
      121 cgatgaatta taatggtggt ggagatccca agtatgcaat aaaacttcag ttcgtttata
      181 cattgctcac aaaaaacaga gtgttatgag aatgggaaaa ataacaatta ttctactgct
      241 tacattctca tgtacaaaat gtctggcatt tgacatttcc atgtctaaag aatgtagtgg
      301 cgttgatgaa cttgaatttg ttgattgtgt taaaaattca tttattatga gcactattac
      361 acttaaaaaa gcagaagtaa atataaaaga gaaaattaaa aaatggaata acgatgagga
      421 cgaagcaata agaaaagaag cagttatttc tttaaaaaga ctagaatctt ctaatcggga
      481 atttatacgt tacagatttg ctcaatgctc ttttgctcta acctgggggg ccagagtagg
      541 acgtctggca ttctctcgac tatatccatg ctatactgag ttaaacaaaa taagagccat
      601 gcaactaaac aatgcttaca tttcagattt tcaaaatcca gatgagatat ccagcaaatg
      661 attattataa aattaacatt actatttata ctggccacaa ttacatattc agtacaatca
      721 gacgaattcg atgatcaaac cacttacacg caataccgtt atttttccac agccatacca
      781 gaagatatac gtagatttat gttctacgta gattatt
//
LOCUS       DAZGTJ010000324          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000324 DAZGTJ010000000
VERSION     DAZGTJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.330"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..766
                     /locus_tag="ACHK8E_005363"
     CDS             <1..766
                     /locus_tag="ACHK8E_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFP0347443.1"
                     /translation="LLWLAADEAGYVSNDWLTYRQAQQLGGHVRKGEVATLAVIYKDW
                     KKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHLMVKAEGSADALPA
                     DTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQVADYWTTLLHELV
                     HSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGIAGDVQHESYIDGW
                     LKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
BASE COUNT          181 a          190 c          256 g          175 t
ORIGIN      
        1 gttgctgtgg ctggccgcgg atgaagccgg gtatgtcagc aatgactggc tgacgtaccg
       61 tcaggcgcaa caactgggtg gccacgtccg taaaggggag gtggccacgc tggcggtgat
      121 ttacaaagac tggaaaaaac aggctgtgga tgaacacggt aaccggctga cggacagtga
      181 gggggagcct ctcatggaaa tcgtaccggt actgaaatcg aatcctctgt ttaatgtggg
      241 gcagtgtgac ggtttacctg aacatctgat ggtgaaggcg gaaggcagcg cggatgcact
      301 gccggcagat accttcagtg agcgggtgct gcaggtactg aatggcagcg gcgtcagcgt
      361 ctcgtcactt ccacaggaca gggcattcta ccggcctgat cttgaccgta tcgtgatgcc
      421 actgtcgtcg cagtttgacc aggtggctga ttactggacc accctgctgc atgagctggt
      481 acacagtacc ggtcatgaaa agcgcctgcg gcgggtgggg ataagtacct ttaccggtcg
      541 caacagcccg gaatatgcgt ttgaagagct gattgccgag atgggcagtg cttttctgtg
      601 tgcacatctc gggattgccg gtgacgtaca gcacgaaagt tatattgacg gatggcttaa
      661 ggttctgaag tctgacaaaa aagcgctgtt tcgcgcctgc cggcaggcac gtgaagcatc
      721 agagtattta ctgacgcggc gaacttcgca ggagtgcgca gcataatttt tactaccggt
      781 ggcggttata acctgaccgc ca
//
LOCUS       DAZGTJ010000325          801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000325 DAZGTJ010000000
VERSION     DAZGTJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.331"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..232)
                     /locus_tag="ACHK8E_005364"
     CDS             complement(<1..232)
                     /locus_tag="ACHK8E_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0347444.1"
                     /translation="MYFFQKNDCFFYIKDALQNIAENKKQIFTFVIFLTLSFIGITIT
                     DSLIFSVSKKAEEELKTYGDNVISIDIYNQKNI"
     gene            complement(295..657)
                     /locus_tag="ACHK8E_005365"
     CDS             complement(295..657)
                     /locus_tag="ACHK8E_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347445.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWIK"
BASE COUNT          251 a          164 c          116 g          270 t
ORIGIN      
        1 taatgttttt ttgattatag atgtcaatag aaataacatt gtcaccataa gttttgagct
       61 cttcttctgc tttttttgaa acactaaaaa tcaacgaatc agtaattgtt ataccgatga
      121 aactaagtgt taaaaaaatc acaaatgtga aaatttgctt tttattttca gcaatatttt
      181 gcaaagcatc tttaatgtaa aaaaaacaat catttttttg aaaaaaatac accgaatgca
      241 aagattcgca ttcgatgtgt ttaagttatt tattaacgtt tggcaaggta ttttttattt
      301 aatccagcta gctttatacc accagcgttt gatgccaact ttattaactt tttgaacatc
      361 agtttttaat gtcaaaggtg atattggggt aacattaccg ctaaacgtgt caacagtacc
      421 atcattgaac tcatgatgcc catcataaat atggacacct ttcacttttc cttcatttat
      481 accctgcata cacactgagt cagtcgttcg gtataccata taacccgcag aggtaggacc
      541 tgaccccgtc gccataacac aacggctcgg gtctaccctg tctgggttcc agccgctgcc
      601 acccgcaaat gcgttcaaac ttaatcccat cagagatact aaaatatatt tcttcattat
      661 aacctcttat tgttaataga taatatattt gtcatgcaac aagttgcatg tactgcactt
      721 acgctccctc agtcacttac ttatgcaagc tcttgggaac tcacttatac actacctgta
      781 actcgaatta tttagagtat a
//
LOCUS       DAZGTJ010000326          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000326 DAZGTJ010000000
VERSION     DAZGTJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.332"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            195..437
                     /locus_tag="ACHK8E_005366"
     CDS             195..437
                     /locus_tag="ACHK8E_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347446.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKSESRIDGDYIISGGTEYRL"
     gene            complement(485..>778)
                     /locus_tag="ACHK8E_005367"
     CDS             complement(485..>778)
                     /locus_tag="ACHK8E_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0347447.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
BASE COUNT          242 a          189 c          134 g          213 t
ORIGIN      
        1 ttcatctctc ttcactcatg ttataacata acaataagat acattctcag ctagatcatt
       61 tcaacagaat ccccgagccc aagggtaatg tggcatcgca gcctctcatc aaatggaaga
      121 catgtgattg cacatttatc atacaagacg tatgataaca ctgttttatt tatcctgctg
      181 ccgagggtat agatatgaat aacgaatctg aaaaagcaat gtggcgttgt aagcctgtgg
      241 ataatccatg gacgttggat tcattactaa aaaagactat ttctgctgtt ttacctgcat
      301 cagacaaagt ttcacaccaa aataaaaaca aattacgtaa taatgaattc attaaaattg
      361 agcatccatg ctcaaagtct gaatccagaa tcgatggtga ctacatcatt agtggaggaa
      421 cagaataccg tttgtaatta agcaggagaa ttttctgtaa attctcctgt aactttttta
      481 cttcctattt tatacctcga ttagcaatac ttcctgcact cgaagctgca cctttcgctt
      541 ccgaaatacc attcggtaat cctcgactga ttccatcgcc aatatgaaca ccagcccagg
      601 acaacgctcc cagccacact gccggcaaga cgagaaacat cgtccccatc accagattca
      661 ttatcagatc atccgatgtg ttctggaatc ccatcatatt gaaccgggta tgtgtatccg
      721 aactgtacag cgccgtcagc atccagctgt caagccaccg tgcaagctcc caccagaa
//
LOCUS       DAZGTJ010000327          771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000327 DAZGTJ010000000
VERSION     DAZGTJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.333"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(131..640)
                     /locus_tag="ACHK8E_005368"
     CDS             complement(131..640)
                     /locus_tag="ACHK8E_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347448.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          203 a          199 c          183 g          186 t
ORIGIN      
        1 aaagcagtat ttatccgcca gatagtcaat tcgtgtatgc atactgaaga gtattccgta
       61 taaagattca gctggcaaaa ctttatcagt ctgtaaaaac taacggaaga gtcgatattt
      121 ctcccgtcca tcaccggatg attgttgcaa taccccgtgg catcagagac tgaacagccg
      181 tttttaacgc aacgtattgc tctgatgtat caggccggac aacccgaaaa cacctttcca
      241 cccggcattg tccgccagcg cttatcaccg gccaggtctg ttgcagtaaa tccgccactt
      301 gcgaacatgc ttcatcaact gtgacactgg cccgcggatg gcaaacactc gtctggctga
      361 gcagcaacag acatcgtatc ggtgctcctt catgttgttc ccgcaaccag cgtaatacca
      421 ccggcgctga tgggctgacc gtgtgatcac gctccgtaat acgttcgtgc acccgtcggt
      481 gaaaggaact acagaatgtc tgaatctgtt gaccacagac gccttctgtc gaatgaagtg
      541 tgaagtggat tgccagcaga tgcggccagt gatccaccgc ctgctgaaca aaacgccgga
      601 tttcccccgg ctctgaaagt aaggcttcgg ttatttgcac tattttatct ctgttgaact
      661 tggttaagtc ggtgcagacg catcaacaca agtacggttc gatgcaaaca gctgtgactg
      721 gcaatatgaa aggaatgatg aatcagtcag gatgacaaag tgccagctga c
//
LOCUS       DAZGTJ010000328          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000328 DAZGTJ010000000
VERSION     DAZGTJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.334"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            195..434
                     /locus_tag="ACHK8E_005369"
     CDS             195..434
                     /locus_tag="ACHK8E_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347449.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(485..>769)
                     /locus_tag="ACHK8E_005370"
     CDS             complement(485..>769)
                     /locus_tag="ACHK8E_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0347450.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
BASE COUNT          232 a          199 c          131 g          207 t
ORIGIN      
        1 ttcatctctc ttcactcatg ttataacata acaataagat acattctcag ctagatcatt
       61 tcaacagaat ccccgagccc aagggtaatg tggcatcgca gcctctcatc aaatggaaga
      121 catgtgattg cacatttatc atacaagacg tatgataaca ctgtgttatt tatcctgctg
      181 ctgagggtat agatatgaat aacgaatctg aaaaagcaat gtggcgttgt aagcctgtgg
      241 ataatccatg gaccttcact tccctgctga aaaaagcagt ctgtgccgtt ttaccagatt
      301 tcacacctgc tcaagataaa ccccaaaata tgttttcact aagtaatgaa aaagatactg
      361 attattcagt accaatgaga ccctttgcat tatatgaccc gtggaaacat caccatgaca
      421 atgctgattt ctaagaaaag caagggtttc cccaattatc ccagctaagg aaaccctaag
      481 aattttactt attagctaaa tctccacttt ttgctgctgc cttagataca ttacttgttc
      541 ctttgttaat tgcatccccc ataaccgttc cgacacttac cccagcccag gacaacgctc
      601 ccagccacac tgccggcaag acgagaaaca tcgtccccat caccagattc attatcagat
      661 catccgatgt gttctggaat cccatcatat tgaaccgggt atgtgtatcc gaactgtaca
      721 gcgccgtcag catccagctg tcaagccacc gtgcaagctc ccaccagaa
//
LOCUS       DAZGTJ010000329          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000329 DAZGTJ010000000
VERSION     DAZGTJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.335"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>767
                     /locus_tag="ACHK8E_005371"
     CDS             <1..>767
                     /locus_tag="ACHK8E_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0347451.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRME
                     DKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADA
                     QARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          179 a          207 c          236 g          145 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      481 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      541 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      601 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      661 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      721 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZGTJ010000330          764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000330 DAZGTJ010000000
VERSION     DAZGTJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.336"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..149)
                     /locus_tag="ACHK8E_005372"
     CDS             complement(<1..149)
                     /locus_tag="ACHK8E_005372"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0347452.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLL"
     gene            443..763
                     /locus_tag="ACHK8E_005373"
                     /pseudo
     CDS             443..763
                     /locus_tag="ACHK8E_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          197 a          194 c          191 g          182 t
ORIGIN      
        1 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
       61 tcacaggagg cagcaacgag ctgctgttta aattcgggag gataattagg gcagcctttt
      121 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
      181 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtgcgctta
      241 cgaacaaaca cggcgcgttc gtcttcatga aacaccggcc gcctggtgcc acacacgccg
      301 ggaatgctac agcaaaacga ccggtcgtcg ctgcggcagc cctgacagtc tcagcaggct
      361 gattctgagc agtattcagc ccatagaatg agataacccg tatgaatgat gacagacgtc
      421 tacggtaccc gatgtatgga acattttctt tctggctaac ggcatgcccg acacatggtt
      481 ttacaccgat gacgcctccg tcacaaaaga gtgggggtgg gaatattgtg acaatgtgga
      541 agtgttccct ctgtgctata tgcatgacgg tcccgttttc cgggggcgct atattgatga
      601 gctcagcgcc gcacggagca gaatgctcca tatgcaccac atactgccgg aatgaacccg
      661 gtacagagaa aattttcatg acgcagaatg tatccgtgaa attcacgaag cgcccctgca
      721 ggctcccggc attctgcaaa aatacaaacg cacaaaatca taag
//
LOCUS       DAZGTJ010000331          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000331 DAZGTJ010000000
VERSION     DAZGTJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.337"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..450
                     /locus_tag="ACHK8E_005374"
     CDS             <1..450
                     /locus_tag="ACHK8E_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0347453.1"
                     /translation="RGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAV
                     FRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAR
                     EQFTGAVDRIFDTAESVCVASVVRTALRAVRNTVVSVARAVWNWIFF"
BASE COUNT          165 a          211 c          195 g          186 t
ORIGIN      
        1 cggggacatc agcaggtgct gtttgtggtg acgcaggccg acaaaacgga gccctgccat
       61 gaatgggata tggccggtat tcagccttct cctgcacagg cacagaatat tcgcgaaaaa
      121 acggaggcgg tattccgtct gttccggccc gtacatccgg ttgtggccgt atcggcccgc
      181 accggctggg aactggatac gctggtcagt gcgctcatga cagcgcttcc cgaccatgcc
      241 gccagtcccc tgatgacccg actgcaggac gagctgcgca cggaatctgt ccggtctcag
      301 gcccgtgaac agtttaccgg tgcggtggac cggatatttg acacagcgga gagcgtctgt
      361 gttgcctctg ttgtccgtac ggccctgcgc gctgttcgta acacggtggt ctctgttgcc
      421 cgtgcggtat ggaactggat tttcttctga acctgtcgtg actgatgccc tccctgactc
      481 tgagtctgct cacaaaagcg ctgttttcgt tactgtctct cttgtccgtg caatagccaa
      541 acaatagaat aaaacgatcg atacctattt tatcgatcgt ttatatcgat cgataagcta
      601 ataataacct ttgtcagtaa catgcacaga tacgtacaga aagacattca gggaacaaca
      661 gagccacacg tcagaaactt ccgtcagccg gacctctggc actgtaaccc tttacctgcc
      721 ggtatccaca tctgtggata ccggcttttt tattcac
//
LOCUS       DAZGTJ010000332          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000332 DAZGTJ010000000
VERSION     DAZGTJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.338"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            28..486
                     /locus_tag="ACHK8E_005375"
     CDS             28..486
                     /locus_tag="ACHK8E_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347454.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIVLLVCIHSLITTIK"
     gene            586..>750
                     /locus_tag="ACHK8E_005376"
     CDS             586..>750
                     /locus_tag="ACHK8E_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFP0347455.1"
                     /translation="MEITKNKNATIKKQSSVGCFSMFTLSFSITFIPAFLTNRQELIS
                     RGLLVPALFLA"
BASE COUNT          237 a          139 c          122 g          252 t
ORIGIN      
        1 tagaacactt tttatatgag ttactatatg aaaatatact ggacaagaaa acgcattcct
       61 gaactttcag cacttccacc ttcattaaga aagaaaaatt ttactgacgc atataatgca
      121 gcatcttctc atatcgaata ctggataggt gctggcgtct catttatctc gatgatgatt
      181 ttatccagag tatatgactt tcttttaccc acccaagata ctttcccggg tgatatcatc
      241 agaagtttat gtgttgtgtg tccatccata ttaatctggt ttcagttttc ggtatatgtc
      301 atgaggaaat attacagaca tattctgctg cgaggaaaag aaaccgaaac catttcagaa
      361 aggttgatac gtgaggctga tacacgagaa tatgaactat ggcgtccggt ccgtcgcttt
      421 ttctccatta tttttctcat tgttctatta gtttgcattc attcactgat aacaactata
      481 aaataaaagt tatggctgtt ctgatacagg aaaataaacg ttcattctat ggttacctct
      541 tcctgttata tagaagaatt ttaaacgaca agtaagggaa tataaatgga aataacaaaa
      601 aataaaaatg caactataaa aaaacagtca tcagtcggct gcttttcaat gtttacctta
      661 tcattttcta ttacctttat tcctgcattc cttacaaaca ggcaggaact tatatccagg
      721 ggacttcttg ttccagcatt atttcttgct
//
LOCUS       DAZGTJ010000333          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000333 DAZGTJ010000000
VERSION     DAZGTJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.339"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..709)
                     /locus_tag="ACHK8E_005377"
     CDS             complement(<1..709)
                     /locus_tag="ACHK8E_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFP0347456.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLVVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREKL"
BASE COUNT          230 a          157 c          130 g          228 t
ORIGIN      
        1 tcaatttttc acgcgcgagt tgattcttgg caatgatctc atgaccttgc tttaaaaata
       61 gaacttcatc attagcgctg atgtgatcaa gatactcgtt cagatatcgc cagtttaaat
      121 cgacatgaat tttatccacc actaacaaat cgtgatcttt gccgttgatg cgtagcgtaa
      181 aagagataca ttttttaccc ggacgatcaa agcaattgta tgccggattc cagctgttgt
      241 tagagtcatc tgaagccatc gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa
      301 cccgccctga ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt
      361 tatcattcgc cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg
      421 agtctaaaaa tggcatcatt tccattttca gaattacggc gcgttgaatc tcctctgtat
      481 tttttaataa atatagtgag tcctttaatg tcagggccga acgttcaata tctttaaaga
      541 aatagccaac gtttttctga ataagttcaa agcggctatc ggcacgtttt accgcgcgtt
      601 ccgataccag ttgatagtta atgatgaaaa aaagaccaat aaatgacagt gctgccaata
      661 aaaataaaat tatcagcctt atctttatct tttcgtataa actcagcatg ttaatacatt
      721 taacaaaata actgtctgaa cgctg
//
LOCUS       DAZGTJ010000334          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000334 DAZGTJ010000000
VERSION     DAZGTJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.340"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(85..225)
                     /locus_tag="ACHK8E_005378"
     CDS             complement(85..225)
                     /locus_tag="ACHK8E_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0347457.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(543..683)
                     /locus_tag="ACHK8E_005379"
     CDS             complement(543..683)
                     /locus_tag="ACHK8E_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0347458.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHTSPCEFRLNIMWSQFAAFLQC
                     KQ"
BASE COUNT          178 a          186 c          185 g          191 t
ORIGIN      
        1 tttgtgcctt ttgtgtgagg gtgcgacttt cgaccgaacc tttggcaacc ggatagcggg
       61 ggctttcacc cccgctgcgg tttcttacgg tttacactgc aagaacgccg caaattctga
      121 ccacatgatg ttaagtctga actcacacgg agaacggtgt atcatccaga cagtcaacaa
      181 catggttaag cataaaatgc tcacgatgat cgttttttgc ggcatagcgc ttgactcctt
      241 ttacagggag gcgctaacct tccacttgct tagggtagat ggtcagggcc tcgggttaac
      301 attaaagttg actccggggc ctttccacat ccggccttca ggtaatcctt ccagccatca
      361 gccgaaaggc acccgcgtgt aatgtactga tttccctttg tgtgcgcaat gcttgagggg
      421 gacggtaaaa aaacgcccgc atattgcggg caaatacgtc tgtaatccaa tggagaaagg
      481 ggtgccatat cgaccgaacc cggcaaccgg atagcggggg ctttcacccc cgctgcggtt
      541 tcttactgtt tacactgcaa gaacgccgca aattgtgacc acatgatgtt aagcctgaat
      601 tcacacggtg acgtgtgtat catccagaca atcatcaaaa tagttatgca taaaatgctc
      661 actatgatcg ttttttgcgg catagcgctt gactcctttt acagggaggc gctaaccttc
      721 cacttgcgag gtagatgggt
//
LOCUS       DAZGTJ010000335          737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000335 DAZGTJ010000000
VERSION     DAZGTJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.341"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..719
                     /locus_tag="ACHK8E_005380"
     CDS             <1..719
                     /locus_tag="ACHK8E_005380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0347459.1"
                     /translation="ADEIVLVIDGQIPIQLKNVIDKWLLLLPIKTIPLEKNVGLGNAL
                     NIGLNYVTNKLVARMDTDDICVPERFEKQISYFQKNPSTIILGGAIDEYDETFKERRG
                     RRFSCCSHSDIVSYSLFRNPFNHMTVMFNKEFILGIGGYKHHHYMEDYNLWLRCIANG
                     AICSNIDNTLVLARTGDAMIERRRGRDYLKSEIELARLKIKCFPNKKAKIILITFSRL
                     IVRILPQQILSSVYKNIRKK"
BASE COUNT          261 a           93 c          148 g          235 t
ORIGIN      
        1 aggctgatga aatagttttg gtgattgatg gtcaaatccc aattcaatta aaaaatgtta
       61 tagataagtg gttgctatta ttacctataa aaacaatccc actggagaaa aatgttgggc
      121 ttggtaatgc gcttaatatt ggattaaatt atgtcactaa taaattagtt gctaggatgg
      181 atactgatga tatctgtgtt cctgaaaggt ttgagaaaca aataagttat tttcagaaaa
      241 atccaagtac gattatatta ggtggggcca ttgatgaata tgatgagact tttaaagaaa
      301 ggagagggcg tagattttca tgttgctcgc acagtgatat agtgagctat tctctcttta
      361 gaaatccatt caaccatatg actgtaatgt tcaataaaga atttatcttg ggaataggtg
      421 gttacaagca tcatcattac atggaagatt ataatttatg gctaaggtgc attgctaatg
      481 gtgccatatg ttctaatatt gataatacgc tggtccttgc acgaacaggt gatgcaatga
      541 tagaacgaag gaggggaagg gattatttaa aaagtgaaat cgaattggca cgcttgaaaa
      601 ttaagtgttt tccaaataaa aaagcaaaaa tcattttaat aactttttct agacttatag
      661 tacgtatact acctcagcaa atattgtcat cagtgtataa aaatatcaga aaaaaatagt
      721 gagttatttt ccacatg
//
LOCUS       DAZGTJ010000336          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000336 DAZGTJ010000000
VERSION     DAZGTJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.342"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..264
                     /locus_tag="ACHK8E_005381"
     CDS             <1..264
                     /locus_tag="ACHK8E_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP0347460.1"
                     /translation="AQITAAAEDAEIHHDYARALILWQHAYHAATLTLNKNLAVAKID
                     FCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKKKTKEG"
     gene            complement(652..>733)
                     /locus_tag="ACHK8E_005382"
     CDS             complement(652..>733)
                     /locus_tag="ACHK8E_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFP0347461.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          211 a          167 c          175 g          180 t
ORIGIN      
        1 gcgcagataa cggcagcagc tgaagatgcg gaaatacatc atgattatgc ccgggcatta
       61 atattgtggc agcatgcata tcatgctgcc acgctgactc tcaacaaaaa cctggcagtt
      121 gctaaaatcg acttctgtat taaacgtatc cggatgcatg aacaaatgag caggataatc
      181 cggaagactg gcacagacga aatactcttc cgtctgagta aacaccatca tttatatgag
      241 aaaaagaaaa caaaagaggg gtgagaaacc cattgtatgg ggaaaacttg ccatgctcac
      301 tgcgggggca tttttgtact ggctgttcta accgggtaac ccgttgatac acatagtaca
      361 ataatgtttt atgcaggctt acgcgccaga ctgccatcag tagtgcattg cgtgccagct
      421 cgcggacagg ggcagtgacc agccaccaca tgagtaagca tcatttcagc gccatcaggc
      481 agctcctgaa actcctgcga gttgaaaaag catcctcctg cattagcaat actggcttgt
      541 cagtgccagt gtgatataag acgtaatgtc aaagttcgtg gtgttgacat atttctgtcg
      601 caaccaaagt atgctcccgc ccagtatata tctgggcagg agcatcattt attagttctt
      661 tttcctgtcg gggccaaaac attctgtcga tgtggcgcag gttgataacc caacaagggc
      721 attggacgtt aat
//
LOCUS       DAZGTJ010000337          723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000337 DAZGTJ010000000
VERSION     DAZGTJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.343"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..695
                     /locus_tag="ACHK8E_005383"
     CDS             <1..695
                     /locus_tag="ACHK8E_005383"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0347462.1"
                     /translation="YSLVNKYANEVIVISEALKNKIIKYITGNNLNLIYDDVHIQNKP
                     MLNYANSARLRKFIIIGTLCEGKGQKIAIEAMHNLIREGYLCHLKIIGNNRVPYASYL
                     NKIVADYNLSDYVEFMGFRHDLDQIRLDNDVCLIPSLSEAFGRVTIESMAAGMIVVAS
                     DSGASKEIINDGINGFLFSSGSVSDLTSVLKKILDVESNNLECIRKRALVDSQKYTSG
                     HAASSIYNLIIN"
BASE COUNT          247 a           90 c          135 g          251 t
ORIGIN      
        1 attactcgtt ggtaaacaaa tatgctaatg aagttatagt tatatcagaa gcgttaaaaa
       61 ataaaataat aaaatacatt acaggaaata atcttaatct aatatatgat gatgtccata
      121 tccaaaacaa accaatgcta aattatgcta attcggctag gctgcggaag ttcattatta
      181 ttggcacatt gtgtgagggc aaaggtcaaa aaatagccat agaggctatg cataatctga
      241 ttcgtgaagg atatttatgt cacttaaaaa taattggaaa taatagagtt ccatatgcaa
      301 gttatcttaa taaaattgtt gctgattata atttgagtga ttatgtagag tttatgggat
      361 ttaggcatga tcttgatcaa ataaggcttg acaacgatgt ttgcttaatt ccctctcttt
      421 ctgaagcttt tggtagagtt accattgaat caatggctgc aggtatgata gttgttgcta
      481 gtgattctgg ggctagtaaa gagatcatca atgatggtat aaatggtttt ttgttttctt
      541 caggttcggt aagtgatctt actagcgtac ttaaaaaaat ccttgatgta gagtctaata
      601 atttagaatg tataaggaaa agggctttgg ttgactcgca aaaatataca tcaggacatg
      661 cagcttcatc aatttataat ttgatcatta actgattttt tataaacttt gattatcatt
      721 tta
//
LOCUS       DAZGTJ010000338          721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000338 DAZGTJ010000000
VERSION     DAZGTJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.344"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..666
                     /locus_tag="ACHK8E_005384"
     CDS             <1..666
                     /locus_tag="ACHK8E_005384"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP0347463.1"
                     /translation="HPNKGTLEFEIQRRKFKQILEKVGDVSGYIGGVKQIPTKPKNQE
                     IDYKEFVKTRVSVRSFSEKKIILEDILKVIDIARYCPSACNRQAVKLFYSLNSKENEH
                     ILKLQNGSRSFREAVPGLIVITSDLRYQEGSEERNLGFIEGGIWISSLVNSLHAYNIG
                     SCVLNWCVNPETDKKLRDLINIPYNYQIISLLAIGYANNDQLVPFSVRKEAIDFIQNI
                     NLK"
BASE COUNT          272 a           99 c          128 g          222 t
ORIGIN      
        1 catccaaata agggaacgct tgaatttgaa attcaaagaa gaaagttcaa acaaatttta
       61 gagaaagttg gtgatgtatc aggttatata ggtggcgtaa aacaaatacc aacaaaacct
      121 aaaaatcagg aaatagatta taaagaattt gttaaaacac gcgttagcgt tagatcattt
      181 tcagaaaaaa aaataattct cgaggatatc ttaaaagtca ttgatattgc aaggtattgt
      241 ccatctgcct gcaacaggca agccgttaag ttattttatt ctttaaattc taaagaaaac
      301 gagcacatac taaagctaca aaatggtagt cgttcattcc gtgaagctgt tcctggctta
      361 atagtaataa catcggattt aagatatcaa gagggtagcg aagagcgaaa tttaggtttc
      421 attgaaggcg gtatttggat ttcttcactt gtgaattcac tccatgcgta taatatagga
      481 agttgtgttt taaattggtg tgttaatcca gaaactgata aaaagttgcg agatttgata
      541 aatatccctt ataactatca gattatttca ttacttgcaa ttggatatgc taataatgat
      601 cagcttgttc cattttcagt aagaaaagaa gctatcgatt tcatacaaaa cattaatttg
      661 aaatgagcat aaaaatgaaa gctaatacgt caaatacgaa aagactattt ttcaatagta
      721 t
//
LOCUS       DAZGTJ010000339          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000339 DAZGTJ010000000
VERSION     DAZGTJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.345"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          205 a          130 c          178 g          206 t
ORIGIN      
        1 agctaattag agatgaaatt aacaaagaac atttgacagc tcatcaaaat agaataaatg
       61 ttgctgaggt tgctgattct gacaagagcg aatttagtag aattaaaacc tcttcggatt
      121 ttgatggggc attatggact gggataagaa agcttatttc attctttaaa aaatagatct
      181 atagcgtatc gttccggcga actcatcctg atgcgttttc ggggggccat cggtgaaaca
      241 tacgaatgat cgccctctga cgatggatga tttattttgt tgcacagaag cgatcgctgg
      301 gcaggtaccg gaggttggct ctgaagctct aattgcgtca ggttgcgaag tgttgacacg
      361 ggtattggtg agaactgccc cgggaacgac tctgggctga ttctggttct gattcgctgt
      421 cagcccgtgt ggttactggc ctggctgatt tcctgtcgtc aggcgtttaa ctcccgactg
      481 caggtatcac ttaccaagag aaggtaaatc cttttagacg tacagtgaaa gttgtctggt
      541 cataatctga agcatctgac aaagttgtgt ccaccaaata ataagtagtg ggaaccaaag
      601 tgtcatatat acagaaaaat ataactcacc gatttccttc acgttaatac gcagggggag
      661 gtatataaca gtccgttcag gtattggata aatatttaga gtggaatgat tacagttat
//
LOCUS       DAZGTJ010000340          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000340 DAZGTJ010000000
VERSION     DAZGTJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.346"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(230..508)
                     /locus_tag="ACHK8E_005385"
     CDS             complement(230..508)
                     /locus_tag="ACHK8E_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347464.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
BASE COUNT          181 a          193 c          147 g          194 t
ORIGIN      
        1 tgaaaagcag ggcgcagatg cccggcaatc agaagcattg tgccggcagg tatgacagag
       61 ttcctgtgct ccagacaaag cattgtctgt ttgagagcca taatgacaac gcaggtacag
      121 ggcatcggat atccggtaaa tatgttgatt tattaaagag acagacatgc cccgtatata
      181 aaattccgtg tcaccgggtg ctgaaaaaaa ctgcaccgac ggcgctcttt tactcctccg
      241 ttacggatat ttcagccccc ttttcaggag cggtatcctt cacctcgctg agcccggact
      301 gagccgcttc agaaacctct gccacctttc cggcagcctg cattttcact tcctccgcgg
      361 catccttcac atcactgagt tgagtctgcg cagcttcctt tatatctgcc gccttcttac
      421 ttacattctc ctttgtcgca tcatcacagc ccgcaaccag aaatggtgcg gaaatacata
      481 acgccagaat tgttttttca gcatacaatc tctcattatt aagtgttaac gcagaacagc
      541 ttatatttcg tccccgggaa atggtcaaaa gacaaccaga acatcacagt gtcagggcac
      601 actcccggta tatgctttta taaccataac atgctgttcc tgatattacc ggaaatgtgc
      661 ccactacatt taacctgttt tcagcaccgg gtcatcggct gtgctgtatt tttta
//
LOCUS       DAZGTJ010000341          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000341 DAZGTJ010000000
VERSION     DAZGTJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.347"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(42..275)
                     /locus_tag="ACHK8E_005386"
     CDS             complement(42..275)
                     /locus_tag="ACHK8E_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347465.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(370..>715)
                     /locus_tag="ACHK8E_005387"
     CDS             complement(370..>715)
                     /locus_tag="ACHK8E_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0347466.1"
                     /translation="FGCVYSLKASLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAR
                     EVKAPRERLKYALEAGLLVTALDGLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNL
                     TGTTRKIPAYHL"
BASE COUNT          171 a          220 c          157 g          167 t
ORIGIN      
        1 tgccggcaat cacactgcct gatggcagca taatccactt attaattctc atccccggag
       61 acaagccctg aaaaccactc gggatgttca ctgtatgctg cgttcagagc atcccaggaa
      121 acgttcatac agaagctgtc ctgcaatttg caggcaacca cctctgccag gcggttccgg
      181 ttatccgcca gtccggcata acctctctct gccagccact cattcagttc atgttccaga
      241 gacagattga taaaatcgta atcggacctt gccattcccg tttccttaac acatgtcttc
      301 agactcaggg ataaagtata aaaatgtcgc cctgctgctg ccagagcaat attccccctg
      361 atggccgtat cagagatggt atgccggtat tttacgggtg gttcccgtca ggttatcgtg
      421 tacctccacg ctcgtagtca ccaccggcat tccggccttt ctcagcctca aaacatcagc
      481 tgcaatacgc tgactaccag accagaacag gccgtccagt gcagtcacca gtaaccccgc
      541 ctccagcgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa cctgctgata
      601 tggcaatgat gtgttttcac tcccgaccat ccggcgaata cgatgagaca gatgataccg
      661 gtatgtatcc ggcacacctg aaaggctggc cttcaggctg tacacgcagc caaag
//
LOCUS       DAZGTJ010000342          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000342 DAZGTJ010000000
VERSION     DAZGTJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.348"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..292
                     /locus_tag="ACHK8E_005388"
     CDS             <1..292
                     /locus_tag="ACHK8E_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFP0347467.1"
                     /translation="SWIKVDSKGYWRNDSNVFPDRVYVGWMLYMPHILLPELIPEAAK
                     IVPVFEGEKQKGTIVVSTEDVFDGNNKEHIGKANDIEIRLLDLGLLPLLTEL"
     gene            547..>686
                     /locus_tag="ACHK8E_005389"
     CDS             547..>686
                     /locus_tag="ACHK8E_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0347468.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRA"
BASE COUNT          225 a          114 c          143 g          204 t
ORIGIN      
        1 ttcatggata aaagtcgact caaaaggtta ttggcgtaat gacagtaatg tttttcctga
       61 cagggtatat gtgggatgga tgttatatat gccccacata ctcttacctg aattaatccc
      121 tgaagccgca aaaatagtac cggttttcga gggcgagaag caaaagggaa ctattgttgt
      181 ttcaaccgaa gacgtatttg acggaaataa taaggaacac atcggaaaag ctaatgatat
      241 tgaaataaga cttctggatc ttggactgct acctttgcta acagagcttt aatccaacct
      301 tatcactcat gggtagatga cgggacagtt acacccgtta atggccttat ttttaacaca
      361 tcagataaaa ttgaatgctc accattaaga atacctggag aaattaaaaa acggagattg
      421 tttcaagagc ttcttttatt taagggttta caggtcaaaa atgtgaatag actagtatca
      481 tgactgttgt atagctgtat taaattagat atttaacatg atagtaatac aaattggatt
      541 attattatga accgaaaatc cccatcatta tatgaaattc tctatggcaa tttcactggc
      601 gggctggagt taaatcaggt cagtgaggaa gagcaggtta ttctttcggt gctcgataac
      661 atgcagcgta ttctgaatac ccgggc
//
LOCUS       DAZGTJ010000343          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000343 DAZGTJ010000000
VERSION     DAZGTJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.349"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..248
                     /locus_tag="ACHK8E_005390"
     CDS             <1..248
                     /locus_tag="ACHK8E_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347469.1"
                     /translation="LMGMRYKGWESGYEDAFVYGLGEYRLVLKFSPGFNHYNVDSKAL
                     MSFRSLRVYRDNKSVTFAELDVFDLSEALSAPDVIFH"
     gene            310..663
                     /locus_tag="ACHK8E_005391"
     CDS             310..663
                     /locus_tag="ACHK8E_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347470.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRKLQNIFNQ
                     LIEYYFIKGGSGFPP"
BASE COUNT          188 a          139 c          156 g          198 t
ORIGIN      
        1 agcttatggg aatgcgatac aaaggctggg agtcaggcta tgaggacgca tttgtctatg
       61 gcctgggcga gtaccggctg gtccttaagt tttcacccgg ttttaaccac tacaatgttg
      121 atagcaaagc gttaatgagc ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca
      181 cttttgccga acttgatgtg tttgatttga gtgaggcgtt aagcgcacct gacgtcattt
      241 tccattaaaa acacagcggg cagtgatgca actgcccgtt atcaacacga cttaccagcg
      301 gggaaagcga tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta
      361 cctgaagatc cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt
      421 tccgtctatc gactggagcg attccggcta cgtccaacat ttcatcaacg tgatcgagaa
      481 gatgctgacc cgctaataaa tgatgcgttg atttatataa gagatgagtg tattgatgag
      541 cggaaattac gaggtgaatc acctgaaact gtaatagcaa tttttaatcg taaactacag
      601 aatatattca accaactaat agaatattac tttattaaag ggggaagcgg ttttccccct
      661 tagttgcgtt tcaatttgcc c
//
LOCUS       DAZGTJ010000344          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000344 DAZGTJ010000000
VERSION     DAZGTJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.350"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..454)
                     /locus_tag="ACHK8E_005392"
     CDS             complement(<1..454)
                     /locus_tag="ACHK8E_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFP0347471.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNAR"
BASE COUNT          173 a          148 c          155 g          205 t
ORIGIN      
        1 ggcgggcatt tttatcctta acggtgttac ccaggcggtt actggtggta tcgaaatcag
       61 caaacaaacc cttgatgtca gcttctgaag gataaccgta agcagaactt tcgatagcaa
      121 cgaagatgct gtttaaatct gcattcagtc tgtcattggt atttgctttc gcagctacgt
      181 tgcagaaaag ctgactgggg tagatgaagt agcctttagt cctgatggca tcgtctttaa
      241 tgtcatcagt aattacgttg tcatccagtt ttgcataaca gatgctgtca tcaccatttt
      301 ccatatggct ggaaaaattt tcgctgataa aacggtagaa aagtgcgccc agaacgtatt
      361 gcttaaaatc ccatccatca acagaacctc tgacatcatt ggcaatttgc catatctggc
      421 ggtgcagttc tgctcgttgt tgaatacttg tcatttttaa tccattttcc tggtctggac
      481 ctgaatcatt gggggcaatt ctacagcaat cttgtggttc aggcagcccc ggagtaagtc
      541 ggtgtacatt gtgagatacc tggggtattc acgacaagga ggaggaacgg aatactgcct
      601 ttcctttgca gtgcaccagg tacattctaa ataatttcag acagatatta tcgttgtgag
      661 tcccccgcct cctttccggt g
//
LOCUS       DAZGTJ010000345          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000345 DAZGTJ010000000
VERSION     DAZGTJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.351"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>679
                     /locus_tag="ACHK8E_005393"
     CDS             <1..>679
                     /locus_tag="ACHK8E_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HFP0347472.1"
                     /translation="GSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRE
                     TGRESAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQL
                     PSLDSSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLW
                     QGLSHAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEE
                     DVMYGDEA"
BASE COUNT          128 a          190 c          218 g          143 t
ORIGIN      
        1 ggatccgccg tggcagactg gccggaagcg gagaagccgt cttttgcggg gctttgttcc
       61 gcgcttgaga accgcctggc acgttcaggg ggcatggagg cactggtccc ccagaacagc
      121 agtccccggg agaccgggcg tgaatcagct cccccggact caccacaact ggcagcggtg
      181 acctcggggc gcgacttact cgaccaggcc aggttacttt cccgctggct gagcgggcag
      241 ccgcagggct ggctggcttc tcaccggctg atgaagacgg tgcggtggga tacggtcgat
      301 cagctccctt cgctggacag tagcggacgc acccgcctgg tccccccgaa aaccgggtat
      361 cgcgctcagc ttaagcgact ctggcttcag aaaaactggg ttgagctgat tgaactggcc
      421 agtcagatgt attgcgaagg tgtgaatcat ttctggctcg atcttcagtg gtatctctgg
      481 cagggactca gtcatgctgg tcagccctgg gattcctggt ctgacgctgt cctgtcagat
      541 ctgcggctgt tgcttaaacg tctcccgggc cttgaaggac tggcatggaa tgacggtact
      601 ccctttgctg acgaggtcac cctcgcctgg atcgctgaaa aagtgaatga agaagatgtg
      661 atgtacggcg atgaagctg
//
LOCUS       DAZGTJ010000346          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000346 DAZGTJ010000000
VERSION     DAZGTJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.352"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..430
                     /locus_tag="ACHK8E_005394"
     CDS             <1..430
                     /locus_tag="ACHK8E_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0347473.1"
                     /translation="FVVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTDAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          147 a          191 c          177 g          161 t
ORIGIN      
        1 gtttgtggtg acgcaggccg acaaaacgga gccctgccat gaatgggata tggccggcat
       61 tcagccttct cccgcacagg aacagaacat tcgtgagaaa acagatgcgg tattccggct
      121 gttccggccc gtacatccgg ttgtggccgt atcggcccgc accggctggg aactggatac
      181 gctggtcagt gcgctcatga cagcacttcc cgaccatgcc gccagtcccc tgatgacccg
      241 cctgcaggac gagctgcgca cggagtctgt ccgctcgcag gcccgtgaac agtttaccgg
      301 tgcggtggac cggatatttg acacagcgga gagcgtctgt gttgcctctg ttgtccgtac
      361 ggccctgcgc gctgttcgtg acaccgtggt ctctgttgcc cgcgcggtat ggaactggat
      421 cttcttctga acctgttgtg gatgatgtcc tccctgcctc tgagtctgct cacaaaagcg
      481 ctgttttcgt tactgtctct cttgtccgtg caatagccaa ataatagaat aaaacgatcg
      541 atacctattt tatcgatcgt ttatatcgat cgataagcta ataataacct ttgtcagtaa
      601 catgctcaga tacgtacaga aagacattca gggaacaaca gagccacacg tcagaaactt
      661 ccgtcagccg gacctc
//
LOCUS       DAZGTJ010000347          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000347 DAZGTJ010000000
VERSION     DAZGTJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.353"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..676)
                     /locus_tag="ACHK8E_005395"
                     /pseudo
     CDS             complement(1..676)
                     /locus_tag="ACHK8E_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737686.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          197 a          119 c          104 g          256 t
ORIGIN      
        1 tttcatattc actacaaaaa aaactctttt tactttcatc ccattctgaa gcatcaattt
       61 caagtttata tacttgcctg gtacctttct ttctggctaa aaaagtaagg taatatctat
      121 tcgactttac ctccagtcgt atattagatt gaattttaca aaaataaatg gctggtttat
      181 tatatctaat tccttcttta tagataatta actgaaaagt atctttataa tttctaactc
      241 cataaggggg aattgataat ggttcataga gattatctcc attaatgtag tagtctacta
      301 ccggtttgat gcttgaagat gacacagggt gtactttgcc ccccaattct ttttcatcat
      361 cttgcgcctg tttattaatt cttctgccag aagagtcgta atttccttca ggtacatcaa
      421 gtttcagaag atatatagcc agtgccggtg gtgaattatg ctttttatta ttctttgagt
      481 tatacttatt ttactcttag gatcatgaat cttacatccc ggcctacgag gctctttata
      541 tcgaaagtat gattgattta aatttatttc tttgaagcta catggagtag caggtatcct
      601 acatttgtca tacggacatt caaaagtggt tttttgtata taaagaacat ccatttcccg
      661 aagatcatga ctatga
//
LOCUS       DAZGTJ010000348          674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000348 DAZGTJ010000000
VERSION     DAZGTJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.354"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>674
                     /locus_tag="ACHK8E_005396"
     CDS             <1..>674
                     /locus_tag="ACHK8E_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HFP0347474.1"
                     /translation="YVNNIADLYIRYAGKVISEQMPELENAILNNMNVLGEADVPRTM
                     QNQPDAVRIWAIEVALSSEAGKKLYDPILDGLRSAVRYDRTYFDKIVASLLPLLEKLT
                     TGKTAELLAPDYLDMSDTRPVFDWEQVIRKKAVVYIGLDALSDSEVASAVGNSMFADL
                     VSVAGHIYKHGMNAGLPGGNGTKNPVNLHCDEFNELMGDEFIPLINKGGGAGMQVTAY
                     TQTSSD"
BASE COUNT          169 a          142 c          200 g          163 t
ORIGIN      
        1 tatgtgaata atattgccga cctttatatc cgttatgctg gtaaggtgat cagcgaacag
       61 atgccggaac tggaaaacgc cattctgaat aacatgaatg tactggggga agcggatgtt
      121 ccccggacca tgcagaacca gccggatgct gtgcgtatct gggccattga ggtggctctc
      181 agcagtgaag ccggaaagaa actctatgat ccgatactgg atggtctgcg aagcgcggta
      241 cgttatgatc gtacctattt tgacaaaatt gtggcatccc tgttgccgtt gctggaaaaa
      301 ctgaccacag gaaagacggc agaactgctg gcacctgact acctggatat gagcgatacc
      361 cgtcccgtgt ttgactggga gcaggtgata cgtaaaaagg cggtggtgta cattggtctt
      421 gatgcgctga gtgacagtga ggtggccagt gccgtgggta actccatgtt tgcggatctg
      481 gtgagtgtcg ccgggcatat ctacaaacac ggaatgaatg ccggtcttcc gggagggaat
      541 ggtaccaaaa atccggttaa cctgcattgt gatgagttta atgaactgat gggcgatgag
      601 ttcattccgt taatcaacaa gggaggcggt gccggtatgc aggtgacggc ttacacgcag
      661 acctcttctg atat
//
LOCUS       DAZGTJ010000349          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000349 DAZGTJ010000000
VERSION     DAZGTJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.355"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHK8E_005397"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHK8E_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFP0347475.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZGTJ010000350          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000350 DAZGTJ010000000
VERSION     DAZGTJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.356"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>668
                     /gene="wzx"
                     /locus_tag="ACHK8E_005398"
     CDS             <1..>668
                     /gene="wzx"
                     /locus_tag="ACHK8E_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158227887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O146 family O-antigen flippase"
                     /protein_id="HFP0347476.1"
                     /translation="YISRVTINTLGIEKFGLYSVISGFVLLAGVFTNILESTSQRFLS
                     KSLGEGGNVSSIFESCISTHIVLSLIAALLIEMLGVWFINYKLRQDVISINEVYIIFH
                     LSVITFVVTVITSPFYAALISFEDVNYYSIFTLCDVISKVVGISLAPQLPWEITINYA
                     AMILITTLVNRVILTSCFVMRYPKLGCRPSLDFCQMKNILSFAVWNMWGGAATVLNSQ
                     GINIV"
BASE COUNT          205 a           91 c          123 g          249 t
ORIGIN      
        1 tatatatcca gggtgaccat aaacacactt ggaatagaaa agtttgggtt gtatagtgtt
       61 atttctggat ttgttctatt agctggagtc tttactaata tattagagtc aacatcacaa
      121 agatttttat ctaagtcttt aggagaaggt ggtaatgttt catcgatctt tgagtcttgc
      181 atcagtactc atatagttct ttcattgatc gctgcattat taatcgagat gttgggagta
      241 tggtttataa attataagtt aaggcaagat gtcatatcaa taaatgaagt ttatataata
      301 tttcatctct cagtaataac attcgtcgtt actgtaataa cttctccatt ttatgcagct
      361 cttatatcat tcgaagatgt taattactat agtatcttca ctttatgcga tgttatatct
      421 aaagtggtag gaataagttt ggcgccacaa ttaccatggg aaatcactat taattatgct
      481 gctatgatac taattacaac attggttaat agggtaattc ttactagttg ttttgtaatg
      541 cgctatccta aactaggttg tagaccttct ctagattttt gtcaaatgaa aaatatatta
      601 tcatttgcag tatggaatat gtggggagga gctgcgacag tattgaactc tcagggaata
      661 aatattgt
//
LOCUS       DAZGTJ010000351          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000351 DAZGTJ010000000
VERSION     DAZGTJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.357"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            256..>657
                     /locus_tag="ACHK8E_005399"
     CDS             256..>657
                     /locus_tag="ACHK8E_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0347477.1"
                     /translation="MAIINLNIERNNITNILKLAKPLVSSPFIIRNIAVSNGQYVNSG
                     DDIVDIELLDKFFIDVKIDPVSEQGNIKEKKIRYHSLVNGLSGLASVVRITRTFENGQ
                     DDKSSGLRVVTLAIDGDHKKLAELLDTAFEIE"
BASE COUNT          245 a           89 c          126 g          197 t
ORIGIN      
        1 agagcccata ctctggaatt gtgaaattat cggatatgga agaaggcgaa ataaaacaga
       61 acgtcacatt atttgatgtg ttgaattatg agtataattc caagattctt ttaattgaaa
      121 aaaaactaca ggctgaatgt aaaaaaaaaa cgtagacttc ataatgacta taaagagagt
      181 aagcattcct atgagttggg atatatatcc aaagatgaat tacttgacat agaagataaa
      241 ataaatgatt cgaacatggc tattataaat ctgaatatag agagaaataa tattactaat
      301 atattaaaac ttgcaaaacc gttggtatca tcgcctttta tcataaggaa tattgctgta
      361 agcaatgggc aatatgtcaa ttcaggagac gatatcgtag atattgaact tctggataag
      421 tttttcattg atgttaaaat tgatcctgtg tctgaacaag gaaatatcaa agaaaaaaag
      481 ataagatatc actctttggt caatggatta tccggtttag catctgtagt ccgcatcacc
      541 cgtaccttcg aaaatggcca ggatgataaa agttctggtt tgagggttgt aacattggcc
      601 attgatggtg atcataaaaa actcgctgaa ttactcgata ctgcttttga gattgaa
//
LOCUS       DAZGTJ010000352          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000352 DAZGTJ010000000
VERSION     DAZGTJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.358"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(190..>657)
                     /locus_tag="ACHK8E_005400"
     CDS             complement(190..>657)
                     /locus_tag="ACHK8E_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0347478.1"
                     /translation="LGRGSFRLDVFFTLFSLIMIIQLFVPYLLAVRKTEEWMISQMSF
                     SGAILWINIFSSVLLVPVYEEIVFRGCLFNSFKFWFNDNIYISAIVTSVIFSALHLQY
                     TDFRTFLMLFLISLVLISAKIKSNGLLMPILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT          252 a          104 c          101 g          200 t
ORIGIN      
        1 ctctttctac gagaaaaggc gaagatattt gttcactttt gagtaattta taatccatta
       61 tatcttaatc agatgtatat cataaacaaa tatgagatta tctaaatata ttagtaaacg
      121 attccttata aggaattatt tgcttctttt tccaacttca ggtgttatag atatctcata
      181 ataacaattt cagactgtta tgtgatattg taacgtatat tgtattcctg ttatgactgc
      241 attcatcgcc atgtgaagta atatgggcat taacaggcca ttcgacttta tttttgcact
      301 gataagaact aacgatatca gaaagagcat caaaaatgta cgaaagtcag tatattgtag
      361 atgtaatgca gaaaatatga ctgatgttac aatagcagag atgtaaatat tgtcattaaa
      421 ccagaattta aaagagttaa aaagacaccc tcgaaataca atctcttcat atacaggaac
      481 aagaagtacg gaagaaaata tattaatcca taaaatggcc ccggaaaaag acatttgcga
      541 gatcatccat tcttccgttt tcctcactgc cagcagataa gggacaaata actgaataat
      601 cattattaaa gagaaaagag taaaaaaaac atccagtcga aaagaacctc ttcctaa
//
LOCUS       DAZGTJ010000353          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000353 DAZGTJ010000000
VERSION     DAZGTJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.359"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..249
                     /locus_tag="ACHK8E_005401"
     CDS             <1..249
                     /locus_tag="ACHK8E_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFP0347479.1"
                     /translation="KDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAV
                     ATVNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
     gene            246..>650
                     /locus_tag="ACHK8E_005402"
     CDS             246..>650
                     /locus_tag="ACHK8E_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347480.1"
                     /translation="MIELFNTSINIHSLKERDITIDDCLNELYRSTVVVKNLTQHDHI
                     WYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRPLINESIWNGQPDGLACSIS
                     HFMKFIDNPKKLNLIIDIDQRVANTSDMIDSLL"
BASE COUNT          226 a          108 c          130 g          186 t
ORIGIN      
        1 aaagataagc tgtgtcagtt ctgggaagct aaagcgcggt atgaccaatt tttcagggct
       61 gatggttcaa agaaaccctg gtggacaggt aatctaagcg caattaatca agcatcccga
      121 catcaggcgg ttgcaacagt caatcagcca ttgaaagttg tctggatatt tatgcaacct
      181 gtaagttgtg agtatttcag gaaaatgttt aaagattaca aggatattat tacgaggtgg
      241 caaccgtgat tgaattattt aacacgtcaa taaatataca ttctttgaaa gaaagagaca
      301 taacgataga cgattgtttg aatgaactat acagaagtac cgtagttgtt aaaaatctaa
      361 cgcagcacga tcacatatgg tacttaactg gctactcaag aaaggacgct tcaaagcatg
      421 tcgtgtttag tgataatgaa ccgaaccaaa atacaataga tacctttgag cggcattata
      481 aaaagaatcg tcctttaatt aatgaatcta tatggaatgg acaacccgat gggctggctt
      541 gctcaatatc acattttatg aaatttattg ataaccctaa aaaactaaat cttatcatag
      601 atattgatca gagagttgcc aatacttcgg atatgattga ttccttgctt
//
LOCUS       DAZGTJ010000354          631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000354 DAZGTJ010000000
VERSION     DAZGTJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.360"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(46..369)
                     /locus_tag="ACHK8E_005403"
     CDS             complement(46..369)
                     /locus_tag="ACHK8E_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0347481.1"
                     /translation="MKWIVIDTLIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESEIPNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          220 a           94 c           93 g          224 t
ORIGIN      
        1 gtggatatta ttaatagcat gtcgagcata tttatatgaa gcccattact tgagtccata
       61 tgggcatatt tttataatac aactattatg caaacattta tttggtattt cgctttctcc
      121 aggaggacac tcttgactgc ttttgagtaa actccataaa tccttgttaa atggtgcgat
      181 gttataaata gtaatagggt attctttatc cttaaggata ataccagact taaccggtgt
      241 aaatatactt ccaggaggga gaaatatagt agattgatac cagatgatca ttttcatatc
      301 accccatatg gctgaaaaag atatgccgca tgtaggttga attaacgtgt caattactat
      361 ccacttcatt tgttatgtct tatcccacgg tatttaatat agttcattag gatgttcatt
      421 tcttgatttt gcatatgagt atattatccc ctcaaaaata ttaattaaaa attaaaaccg
      481 ttgcctatat aaaacaaaat ttaaagcaag aaatctcaat agatattaaa caaaatgata
      541 tttagtgaaa acaataaatt attcacttcg ttttagattt gtttagctat aatgttatac
      601 attcaaatga ctgaacatca tgtaattaaa a
//
LOCUS       DAZGTJ010000355          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000355 DAZGTJ010000000
VERSION     DAZGTJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.361"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..602
                     /locus_tag="ACHK8E_005404"
     CDS             <1..602
                     /locus_tag="ACHK8E_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFP0347482.1"
                     /translation="DSGRITTFLKTHLGGLVHQEGNHWVAEPSASQGMVVSPDFLRAI
                     NQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPG
                     NTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRTQLVWITADGNP
                     LKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
BASE COUNT          130 a          163 c          187 g          144 t
ORIGIN      
        1 gcgactcggg tcggatcacg acgttcctga aaactcatct tggcgggctc gtccaccagg
       61 aggggaatca ctgggtcgct gagccttccg caagccaggg gatggttgtc agtccggatt
      121 tcctccgggc gattaatcag ctggcagagc tgtcagatat cgtgttctct cagggcgatg
      181 cgggggttca cttcgaactg atggcgcgtc cttcacggga tgttgccaga gtgcagctta
      241 cccttgatga acagaatctg gattacttca atcagatgga aagctggcag agcttcacct
      301 ggccggggaa tacttactat ccaggtgtgt cgctcagctg gcggagtgta agcagtggta
      361 tgcaactcta cgccagtcac caggggaact gggggtttat tcgcctgctg gacgaagccc
      421 tgatcacgcc acttgatgcc agccgcacac aactggtgtg gatcactgcc gatggtaatc
      481 ctctgaaatt cgtcatgcgc agcgagctgg gcgacggtcc gcttgcctta ctgaagctac
      541 aggggtttac gctgcccacc gccattttta acgttgccgc cggcacggaa ggtgttgagt
      601 aaaaatgaac aggggcattt atcc
//
LOCUS       DAZGTJ010000356          622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000356 DAZGTJ010000000
VERSION     DAZGTJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.362"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..614)
                     /gene="tssL"
                     /locus_tag="ACHK8E_005405"
     CDS             complement(<1..614)
                     /gene="tssL"
                     /locus_tag="ACHK8E_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP0347483.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILT"
BASE COUNT          147 a          191 c          153 g          131 t
ORIGIN      
        1 gtaagaatga cggcgagcac tgtccagaac cagactgagc gtatcagacg ccagcgatgt
       61 ttctccatcc ggtaaacaac cagggacaga ccaccatcag gcagagaaac acgttcagaa
      121 agggctttca tgacctcatc acgctgtgac tggctgactg ctttcaggcc gtacaccccc
      181 tgaaaaccca gtgctgtcac ccggtgaaag caggttaaaa cggccgggtc cggtgaaggc
      241 tgacgcagaa cctctgcaat gcgatcccac agtgcttcac cagcccgaag agaaccaaaa
      301 aagcgggcct gaagcggcac tttacgccag gccgcctggc cttcgtcaac gtccggctcc
      361 ccgccttccc cgcttttttc aggcttacgg ctcatcaccg tttcgtccag gagggcacat
      421 tgtgcatagg taatatgctc cacactggca tcgtcataac cggcacgctc cagcgcttcc
      481 cgcacacttt cgacctgttt aacgcatttg ttgtaaagcg cagtgccatc gggcgtgagt
      541 gcaccttttt tcagcatggt gacggtaagc catgtctcca tcatcagctg gtcgatatca
      601 atatcctgtc tcatgagcgc aa
//
LOCUS       DAZGTJ010000357          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000357 DAZGTJ010000000
VERSION     DAZGTJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.363"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>616
                     /locus_tag="ACHK8E_005406"
     CDS             <1..>616
                     /locus_tag="ACHK8E_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP0347484.1"
                     /translation="GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVE
                     KSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAID
                     PTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNG
                     TISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLAT"
BASE COUNT          160 a          188 c          168 g          100 t
ORIGIN      
        1 gggcaccacc acgctgaata acggtgccac gcttaccctt tccgggaaaa cggtgaataa
       61 cgataccctg accatccatg aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa
      121 cggcagggtg gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcatcca
      181 gaaaaccgtc aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga
      241 tgtcatcgct cagcgcggca cggccctgaa gctgaccggc agcacagtgc tgaacggtgc
      301 cattgacccc acgaatgtca ccctgacatc cggtgccacc tggaatatcc ccgataacgc
      361 cacggttcag tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccgc
      421 ccgcacaggg aagttcgttc cgacaaccct gcaggtgaaa aacctgaacg ggcagaatgg
      481 caccatcagc ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca gactggtcat
      541 tgacggtggc agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg
      601 tacggggctg gcgacc
//
LOCUS       DAZGTJ010000358          606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000358 DAZGTJ010000000
VERSION     DAZGTJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.364"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..113
                     /locus_tag="ACHK8E_005407"
     CDS             <1..113
                     /locus_tag="ACHK8E_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HFP0347485.1"
                     /translation="IAELNAELKTTVSKIDQLRKDIDAIVAEIEGCEVQK"
     gene            110..>606
                     /locus_tag="ACHK8E_005408"
     CDS             110..>606
                     /locus_tag="ACHK8E_005408"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0347486.1"
                     /translation="MSELSYLEKLLDGAEVEWKTLGKVLKRTKGTKITAGQMKALHKD
                     NAPLKIFAGGKTVAFVDFKDIPEKDINREPSIIVKSRGIIEFEYYDKPFSHKNEMWSY
                     HSNNDAISIKYIYYFLKINEGYFQKIGGKMQMPQIATPDTDKFEVPIPCPDNPEKSLA
                     IQSEIV"
BASE COUNT          217 a          103 c          134 g          152 t
ORIGIN      
        1 atattgctga gctgaatgct gagctgaaaa ccacggtcag caaaatcgac cagttgcgta
       61 aagatattga tgcgattgtg gctgaaattg aaggctgcga ggtgcagaaa tgagcgagtt
      121 gagttatctg gaaaaattgc tggatggggc tgaggttgag tggaaaacac tagggaaagt
      181 gcttaaacga actaaaggca caaaaataac cgctgggcag atgaaagctc ttcacaaaga
      241 taatgcgccg cttaaaattt ttgcaggagg aaaaacagta gcctttgttg actttaaaga
      301 tataccagag aaagatatta atagagagcc atcgattatt gttaaatcca gaggtattat
      361 cgaattcgag tattacgata aaccattctc tcacaaaaat gagatgtggt catatcactc
      421 aaataatgat gcgataagca taaagtatat ttattacttc ctaaaaataa atgaaggata
      481 tttccagaaa attgggggta aaatgcaaat gccacaaatt gcaactcctg atacagataa
      541 gtttgaagtt ccaatcccct gcccggacaa tccggaaaaa tcccttgcca tccagtctga
      601 aatcgt
//
LOCUS       DAZGTJ010000359          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000359 DAZGTJ010000000
VERSION     DAZGTJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.365"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..268)
                     /locus_tag="ACHK8E_005409"
     CDS             complement(<1..268)
                     /locus_tag="ACHK8E_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP0347487.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(392..>604)
                     /locus_tag="ACHK8E_005410"
     CDS             complement(392..>604)
                     /locus_tag="ACHK8E_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0347488.1"
                     /translation="AWLFGHTARPLTRTEMVVVFGVIFHEYGCTFLADQLGISMKTVC
                     AHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          180 a          167 c          113 g          144 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt catatcactt atattattct
      121 tatacagaaa gccgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcact ccctccagaa
      241 aataactgtt atcacaaata aaacgcatac ccgttccaca tgaaatttat cacgcctgac
      301 cacataacat taattatccg gagcaccggg atgcccgtga cacagcatgc acagaaattc
      361 ccgtcgcaac agccttcatc agaccggctt tttaccggtc tgcggtgctg cagagatatt
      421 taacactgac acctcgcaga ccgcttttct ccatcgcatt gtaaagatgc gcacataccg
      481 tcttcatgct tatccccagc tggtctgcca gaaaggtaca gccgtactca tggaaaataa
      541 ccccgaagac caccaccatc tcggtacggg tcagtggtct tgccgtatgt ccgaacagcc
      601 acgc
//
LOCUS       DAZGTJ010000360          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000360 DAZGTJ010000000
VERSION     DAZGTJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.366"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>588)
                     /locus_tag="ACHK8E_005411"
     CDS             complement(<1..>588)
                     /locus_tag="ACHK8E_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0347489.1"
                     /translation="GFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLAD
                     NIRLADDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVS
                     QLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEI
                     DVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGKL"
BASE COUNT          119 a          184 c          136 g          149 t
ORIGIN      
        1 agtttcccgg tcgcagcgtc ttcggtctgt gtgtaaaaac gaccaccaat attgacaagc
       61 gggttcccct ggctgtcggt gtactccacc ggaatccccg tcacatcaat ctcatagctg
      121 taatttccct tgtcatcctt actgatggct gaaacccggg tattctgtcc gtggcggact
      181 tcaactgtct gggaagaaac agcaacagcc tcatcaccgg attcagtctt cagtgcccag
      241 gtcgttgctg ccgttgcctg actgatttca cggttcagtt gtgaaacgtt aacggcgtcc
      301 tggtcattca ccccgtcttt cacgccggaa ataacccggc cagccgcatc aataccgttc
      361 acagtcactg acgggccatc cttgatggtc agtccgtttt tatccatccg ggttccgccg
      421 acagaaacac tgccgtcatc tgccaggcga atgttatcag cgagtctgac cgtcaggctc
      481 ccgttctgaa cagtcgtgct gatattctgc ccgtcaccac caaccgtgat atgcttcccg
      541 agagacgtgt tcaccttgct tccgtcatcc gcctgcagac caaagccg
//
LOCUS       DAZGTJ010000361          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000361 DAZGTJ010000000
VERSION     DAZGTJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.367"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>585)
                     /locus_tag="ACHK8E_005412"
     rRNA            complement(<1..>585)
                     /locus_tag="ACHK8E_005412"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          116 a          189 c          130 g          150 t
ORIGIN      
        1 gcatttcacc gctacacctg gaattctacc cccctctacg agactcaagc ttgccagtat
       61 cagatgcagt tcccaggttg agcccgggga tttcacatct gacttaacaa accgcctgcg
      121 tgcgctttac gcccagtaat tccgattaac gcttgcaccc tccgtattac cgcggctgct
      181 ggcacggagt tagccggtgc ttcttctgcg ggtaacgtca atgagcaaag gtattaactt
      241 tactcccttc ctccccgctg aaagtacttt acaacccgaa ggccttcttc atacacgcgg
      301 catggctgca tcaggcttgc gcccattgtg caatattccc cactgctgcc tcccgtagga
      361 gtctggaccg tgtctcagtt ccagtgtggc tggtcatcct ctcagaccag ctagggatcg
      421 tcgcctaggt gagccgttac cccacctact agctaatccc atctgggcac atccgatggc
      481 aagaggcccg aaggtccccc tctttggtct tgcgacgtta tgcggtatta gctaccgttt
      541 ccagtagtta tccccctcca tcaggcagtt tcccagacat tactc
//
LOCUS       DAZGTJ010000362          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000362 DAZGTJ010000000
VERSION     DAZGTJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.368"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..545)
                     /locus_tag="ACHK8E_005413"
     CDS             complement(<1..545)
                     /locus_tag="ACHK8E_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0347490.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVLRFTLKTLRQQ
                     CKQIKSLTGFTLPVVLSAEFSG"
BASE COUNT          150 a          164 c          150 g          113 t
ORIGIN      
        1 ccggaaaatt cggcactcaa caccaccggc agtgtgaaac ccgtcaggga ttttatctgc
       61 ttacactgct ggcggagtgt tttcagtgta aaacgaagaa ccgcttcatc atgatgacag
      121 tctggcaggc agcggtacat cacagagagc tgaccgacct ggcgcgggaa ctgcgtctgt
      181 atgctgcgga caacgtcagt taagcgactg acgtcaccga cccgcagcca ccatccccgg
      241 gctgttttac gcagcggacc gtcctgaaac aacgcttccg gcatgtctcc acataccagt
      301 acaacagggc cccgggtgtc ggataatgcc ggttcagcat ccgtcagggt aacgtcagat
      361 gtgagctttt gcttttcact ccggctgttt ttaaaaacac tccagcctgc cagaagaagg
      421 atcagcagag tagccagacc gttaaaaacg ttgcctgccg ggatgaaata ccacagcaac
      481 cacagcaacg tggaaacggc caccagggca tgcagggcta ccggaatgat gacagatcca
      541 cgcatcagcc atgcaactcc ggtatttgtg atgacaa
//
LOCUS       DAZGTJ010000363          574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000363 DAZGTJ010000000
VERSION     DAZGTJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.369"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(248..>574)
                     /locus_tag="ACHK8E_005414"
     CDS             complement(248..>574)
                     /locus_tag="ACHK8E_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0347491.1"
                     /translation="EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLM
                     TRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAV
                     WNWIFF"
BASE COUNT          144 a          151 c          162 g          117 t
ORIGIN      
        1 gagatccggc tgacggaagt ttctgaattg tggttctgtt gttccctgaa tctcatgtct
       61 gtgtgtatct gcgcacgttg ctaacaggag ttattattag cataacgatc gatattaacg
      121 atcgttgaaa tgggtatcga tcgttttatt ctattatcta gctattgcac ggacaagaga
      181 gacagtaacg aaaacagcgc ttttgtgagc agacccagag gcagggagga catcatccac
      241 gacaggttca gaagaaaatc cagttccata ccgcacgggc aacagagacc accgtgtcac
      301 gaacagcgcg cagggccgta cggacaacag aggcaacaca gacgctctcc gctgtgtcaa
      361 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agagcggaca gattccgtgc
      421 gcagcccgtc ctgcagtcgg gtcatcaggg gactggcggc atggtcggga agcgctgtca
      481 tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg gccacaaccg
      541 gatgtacggg ccggaacaga cggaataccg cctc
//
LOCUS       DAZGTJ010000364          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000364 DAZGTJ010000000
VERSION     DAZGTJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.370"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(96..473)
                     /locus_tag="ACHK8E_005415"
     CDS             complement(96..473)
                     /locus_tag="ACHK8E_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0347492.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
BASE COUNT          136 a          197 c          117 g          105 t
ORIGIN      
        1 ccacagctcc cgccagaaac agcacaggtt cactggcaat ttgatctgac agcgatggat
       61 acagtggtcc cggaaaatcg atatcgctcc tcatatcacc cctctcccag ccacaacagc
      121 agatagaaca aatccgccgc atcagtcact gatatatacc agcggggatg aatatactcc
      181 tcctccgacg gccagctctc atgcccactc acagtaagca gaatatgcac atactgaccc
      241 tgctgacctt ccagcatcac cgccacactg ctgttcagac ccaccctctg tggctggcaa
      301 cggatacact ccacccgccc cagttgcgcc gccagatgcc cgcgaagacg ttcaagatta
      361 tcccgtgtgg tgcaacttat ccccggcgca ccctgtggcg gcagaacaaa cacacttccg
      421 gcggggtgaa cagcagacac cacttccggt tccggatact tcagacacag catagtctca
      481 ctccctgcca gccagaccac gtagcccgtc cagctttcgt gccacatgaa acgctgcttc
      541 cagttcgctg cactg
//
LOCUS       DAZGTJ010000365          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000365 DAZGTJ010000000
VERSION     DAZGTJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.371"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(354..494)
                     /locus_tag="ACHK8E_005416"
     CDS             complement(354..494)
                     /locus_tag="ACHK8E_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0347493.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSEFAAFLQC
                     KQ"
BASE COUNT          133 a          138 c          132 g          146 t
ORIGIN      
        1 gatgatcgtt ttttgcggca tagcgcttga ctccttttac agggaggcgc taaccttcca
       61 cttgtctagg gtaggaaggg ttaggcctcg ggttaaacaa atgttttact cggggccttt
      121 ccacatccgg ccttcaggta ttccttccag ccgtcagccg aaaggcaccc gcatgtaatt
      181 tactgatttc cctttgtgtg cgcaatgctc agtattgaag ctgttgaggg gagacggcaa
      241 aaaaaagccc gcatattgcg ggcaatgaag tcagtacgct aacccaaaag ggtgccatat
      301 cagccgaacc cggcaaccgg atagcggggg gctttcaccc ccgctgcggt ttcttactgt
      361 ttacactgca agaacgccgc aaattctgac cacatgatgt taagcctgaa ctcacacggt
      421 gacttgtgta tcatccagac aatcatcaaa atagttatgc ataaaatgct cactatgatc
      481 gttttttgcg gcatagcgct tgctcctttt tcaaagaggc gctaaccttc cacttgctag
      541 gtagatggt
//
LOCUS       DAZGTJ010000366          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000366 DAZGTJ010000000
VERSION     DAZGTJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.372"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>537)
                     /locus_tag="ACHK8E_005417"
     CDS             complement(<1..>537)
                     /locus_tag="ACHK8E_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0347494.1"
                     /translation="FSVVMRLGAVLSGTPSVWSDRLCSRTCLQFSRNTEQTVPAGMFP
                     DAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALNNRDLIRQVSTDLQRWNA
                     VPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYPGQRLWLALQQAIDTRVP
                     PPSPEPEPVPEIVRLDSLA"
BASE COUNT          130 a          160 c          154 g           93 t
ORIGIN      
        1 gccaggctat cgagtcgtac aatttccggc accggttctg gttcagggga tggtggtgga
       61 acccgggtat cgatagcctg ctgcagggcc agccacagac gttgtcccgg gtaatagccc
      121 aggctgtaac gtaaaggcac tccctgacgc tgccactgct caagcagaag cgcatcctgt
      181 ttcagtgcgg ccagcgattc cgcctcagga cggtagtgcc ccttcgggac agcattccag
      241 cgctgtaaat cggtgcttac ctggcggatc agatcacgat tgttaagcgc agaaatcccc
      301 agcgcagtca ggacacacag ccatatcagc aaaatcagac gacgggtacg ctggcctccc
      361 tgtaccgtcg acgcaaaagg tgccagcagc gacagaaccg catccgggaa catccctgcc
      421 ggcactgtct gctccgtgtt tcgggaaaac tgcaaacagg tacgggaaca gagcctgtcg
      481 gaccagactg acggagtccc cgaaaggacg gcaccaaggc gcattacaac ggaaaat
//
LOCUS       DAZGTJ010000367          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000367 DAZGTJ010000000
VERSION     DAZGTJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.373"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..116
                     /locus_tag="ACHK8E_005418"
     CDS             <1..116
                     /locus_tag="ACHK8E_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0347495.1"
                     /translation="NILTNDNVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            126..537
                     /locus_tag="ACHK8E_005419"
                     /pseudo
     CDS             126..537
                     /locus_tag="ACHK8E_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          160 a           98 c          135 g          144 t
ORIGIN      
        1 ttaatatttt aacgaatgat aatgtattca aggcagggtt aagacgtaag atgcgaaaag
       61 cagccatgga cagaaactac ctggcgtcag tccttgctgg gagcgggctt tcgtaatctt
      121 tccctgggcg gtatatcatc caggactcga ttgggctgaa ggggggatgg aatctgtacc
      181 aatatccgct gaatccagtg caacacgttg atccattagg gcttagtact atgataatag
      241 gtaacggccc cgttcctgat aatccttttg ggcatgctgc ggcggcaaat cgatatggat
      301 taatgagttc gggaactggg gatgagatgg gagcctctgt ctctgattat tttaaaaaga
      361 tgcaaccccg cagagatacc tggatttgga taatagatac aacagatgaa gaagaacaat
      421 gtatgatgaa taaggcaata gaccttaata aaaaactaaa aactattaac ctcgggcctc
      481 ttcctgtttt aaataattgt tttagccgaa cgaacatgat ttttgaggcg tgcgggt
//
LOCUS       DAZGTJ010000368          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000368 DAZGTJ010000000
VERSION     DAZGTJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.374"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>527
                     /locus_tag="ACHK8E_005420"
     CDS             <1..>527
                     /locus_tag="ACHK8E_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFP0347496.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKPEL
                     IPDSTDNKVTVPADKVKNNTEVTATAKDPGGNESAPVTVTSKTDGVADAPALTIPEVA
                     DSVANAAELKDGLQA"
BASE COUNT          157 a          109 c          166 g           95 t
ORIGIN      
        1 gcgaatgtgg gcattcctgc ggataaagtg acgcaggatg gggaatacac agttaaggct
       61 gaaatcacag acccggcagg caacaccagt ggtcagggta aagctaccca gtttggcgtg
      121 gatactgtag ctccgtcaga accagcactg aaggctgaga atgatggttc tgtatcagcg
      181 actctgccag atggtgcgaa caaaggtgac aaggtggaag tgacctttac ggatgaagag
      241 ggtaatgagc agaaagtcac gctggagaaa ggggatggca actggagttc cgacaagcct
      301 gaactgatcc cggacagcac cgataataaa gtgacagttc cggcagacaa agtgaagaac
      361 aacacagagg tgacggcaac agcgaaagat ccgggtggta atgaaagtgc tccggtcact
      421 gtgacgtcaa aaactgatgg tgtggcagat gctccggccc tgaccattcc agaagtggct
      481 gatagtgtgg ccaatgcggc tgagctgaaa gatggtttac aggcaga
//
LOCUS       DAZGTJ010000369          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000369 DAZGTJ010000000
VERSION     DAZGTJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.375"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..207
                     /locus_tag="ACHK8E_005421"
     CDS             <1..207
                     /locus_tag="ACHK8E_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0347497.1"
                     /translation="SEKVSNADIVHHKENNMNLDNVKSPLIIKEYKNEKAILKKIKKL
                     KRNPKAFLIDSKHRSLNLLARFLF"
     gene            279..400
                     /locus_tag="ACHK8E_005422"
                     /pseudo
     CDS             279..400
                     /locus_tag="ACHK8E_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          166 a           96 c          109 g          148 t
ORIGIN      
        1 tcagaaaaag tttccaatgc tgatattgta catcataaag aaaataatat gaatcttgat
       61 aatgttaaat ctccattaat aataaaagaa tataaaaatg aaaaggcaat attaaaaaaa
      121 ataaaaaaac tcaaacgtaa tcctaaagct tttcttattg actctaagca caggtctttg
      181 aatttgttag ccagatttct gttctaattt tggatagcag aatatgcaat gttatttttc
      241 gaatcctcca taactgatat atgtgctgtt agtggagtac aagaataagc tcgcgttagc
      301 agtctgtcag tactgcgaca gcgttgtctg ggagggcttt acccggtcaa aacagcatta
      361 ttgtgcaccc catgtgacgg atgaactgtg tgctcagggg gcattttact gtaaaaaccg
      421 tgctgcggtc agccgtaacc gtcaggtggg tgaacgcaac agtcgcgcca ttgcttttca
      481 ggcacgtacc ctgcaggcgc atgagcgtcg tatgaacag
//
LOCUS       DAZGTJ010000370          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000370 DAZGTJ010000000
VERSION     DAZGTJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.376"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>518
                     /locus_tag="ACHK8E_005423"
     CDS             <1..>518
                     /locus_tag="ACHK8E_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0347498.1"
                     /translation="ARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLVTYRGARD
                     SRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQGRYRVKM
                     DFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDGTEVAIAYSNGDIDLPYIAYALH
                     DAEHPDLVSRDN"
BASE COUNT          105 a          148 c          165 g          100 t
ORIGIN      
        1 gcccgcattc acctgttcag caacgccgca cacctctccc ccgggcaggt gctggagccg
       61 cagggggatg tcattgcgga cctgaaggaa gggattgttc tcacactggt gacttaccgg
      121 ggggcccggg attcccggct gcatgtgtca gcatggggaa tgccttacac cgaacgttac
      181 tgtttccgtc cggcagaaat cccgcgtccg gtcatcccgg gtacgcttcc ggcacggatt
      241 gagagtcgcg ggaagaacga tatctatgcc catctggacg ggcaggggcg ttaccgggtg
      301 aagatggact ttgaccggga ggagacggaa cccggattcg gctacctgtg gctgcggatg
      361 gcaaaaccct gtgcaggtga aacgctgggc tggcatacgc cgctcattga tggcaccgaa
      421 gtggcgattg catacagcaa tggcgatatt gacctgccct acatcgcgta tgcactgcac
      481 gacgctgagc atcctgacct cgtgagccgg gacaatca
//
LOCUS       DAZGTJ010000371          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000371 DAZGTJ010000000
VERSION     DAZGTJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.377"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..399
                     /locus_tag="ACHK8E_005424"
     CDS             <1..399
                     /locus_tag="ACHK8E_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP0347499.1"
                     /translation="DDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIY
                     ATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPE
                     SSTESVGIGAGVDASVMTLACRTWQECWVN"
     gene            411..>515
                     /locus_tag="ACHK8E_005425"
     CDS             411..>515
                     /locus_tag="ACHK8E_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFP0347500.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQ"
BASE COUNT          142 a           70 c          136 g          167 t
ORIGIN      
        1 gatgattcat atggaacatc atcaattgat attactggca gtggagatct aatatcatta
       61 ggtggtcatg caggacttgg cgttgcgttt gctaaaaaga aaggtgaaat gttatctgat
      121 atttgtattt atgctacagc atgtggacat gcaggaattg gtggtgggat aaatgcggct
      181 atcacatatt ctgagaccaa gtctttacct acatcgggag tcagtaattc agtaggtgta
      241 acggttggcg gcggagttgg ggggcatttt gcgtatactt atgtagtgga tgttgataat
      301 ccagaatcat cgacagaatc tgttggtatc ggtgcaggtg ttgacgcttc agttatgact
      361 ctggcttgta gaacgtggca agaatgctgg gtcaattaac aggaggaaat atgaaaaaat
      421 atatattttt agttttattt ttattgtgtg gttgttctcc agtggacata gagccagcta
      481 gacaagaagt taatatcttt cgtggtttat atcag
//
LOCUS       DAZGTJ010000372          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000372 DAZGTJ010000000
VERSION     DAZGTJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.378"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>508)
                     /locus_tag="ACHK8E_005426"
     CDS             complement(<1..>508)
                     /locus_tag="ACHK8E_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0347501.1"
                     /translation="INIFKVCRDELLSYYLPIDSFRRLLTGVPDDRNILLYMMQIDQR
                     TLLLNFAAAWSWLEFWKEKLSKIRNHTYACIFSGAQIYNRTLLELLKTHPTIPLVLEH
                     FFTGNEYYMEEKYDPIPNNTNLKINNTYNSIIIEEHGFELDRERIKAINKVLLSKNKN
                     VRQPSDTLL"
BASE COUNT          158 a           91 c           79 g          180 t
ORIGIN      
        1 agcaatgtgt cagagggttg ccgaacattt ttattcttag acaaaagaac cttattaatt
       61 gcctttatcc tttcccggtc aagttcaaaa ccatgctcct caattataat tgaattatat
      121 gtgttattta tttttaaatt tgtgttatta ggaataggat cgtatttttc ctccatatag
      181 tattcattgc cagtaaaaaa atgttctagt accaggggga ttgtcggatg tgttttcaga
      241 agttctaata gggtcctgtt atatatctgg gcaccagaaa aaatacacgc ataagtatga
      301 tttcttattt tactaagttt ttctttccaa aactcaagcc aggaccatgc tgccgcaaag
      361 tttaataaca atgtcctttg atctatttgc atcatgtaca ataatatatt tctatcatct
      421 ggcacaccgg ttaacaaccg tctaaagcta tcaattggca aataatacga taataattca
      481 tcccggcata ctttaaagat gttaattc
//
LOCUS       DAZGTJ010000373          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000373 DAZGTJ010000000
VERSION     DAZGTJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.379"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>498)
                     /locus_tag="ACHK8E_005427"
     CDS             complement(<1..>498)
                     /locus_tag="ACHK8E_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347502.1"
                     /translation="SHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPENAISKGIAGL
                     SAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESPVTPFVPYFLS
                     TLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQTDDDKAAAVV
                     AQRVA"
BASE COUNT          107 a          151 c          127 g          113 t
ORIGIN      
        1 tcagccaccc gctgggccac caccgcagca gctttatcat catctgtctg ggtcacgaac
       61 ccggagcggg gatacacatt gccccacata ttctcttctt cctggctgcc tatttcgcgt
      121 ttaccgggga tcagggcctc cgggtacagt gactccggta gtcctgtacg ccaggcaata
      181 ctgtcaagcg tactgagaaa ataaggaaca aaaggtgtca ccggtgactc acaggaatat
      241 cccggcacca tccctccgat aagcgtggtg gccgggtgcc ctgttgcatc ggcatattta
      301 aaacggattg acgaacgacg ttccccctgt accttcatgt ctgcggggtt ccctcctcct
      361 gctgacagac cggcaatacc tttgcttatg gcattctccg gaccaccggc tgtactgctg
      421 accgccgaca tttcacgcca gggatttccg ccaggtgcgg tataggtgga aacaaccact
      481 tccgggatat aatgggaa
//
LOCUS       DAZGTJ010000374          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000374 DAZGTJ010000000
VERSION     DAZGTJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.380"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>485
                     /locus_tag="ACHK8E_005428"
     CDS             <1..>485
                     /locus_tag="ACHK8E_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0347503.1"
                     /translation="ICTAKNYISTIDFYIELIDKLLCDDDVFIVFKSHPWERQKNNVR
                     SALTLDKINEHIKSLSLDKSSRVLLTEHFNLEGLIKQSDYIVTLCSQSAIEAAFWGMK
                     PIQLGNAFYGQKGFTHDYDSIDEFYADLQKNKLNKYLTVDEYDNLECFLVKFLEKSLI
                     S"
BASE COUNT          169 a           60 c           84 g          172 t
ORIGIN      
        1 ttatatgtac agcaaaaaat tacatttcca caatagattt ttatattgaa ctgattgata
       61 agttattatg tgatgatgat gtttttattg tgtttaaatc ccatccatgg gaaaggcaaa
      121 aaaataatgt gcgttccgct ttaacattag acaaaataaa tgaacatatt aaatctcttt
      181 cactggataa atcatccaga gtacttttga cagagcattt taatttggag ggtttgatta
      241 aacaatcgga ttacatagtg actttatgct ctcagtctgc aatagaagcc gcattttggg
      301 gaatgaaacc tattcagctt gggaatgcat tttatggaca aaaaggattt actcatgatt
      361 atgatagtat tgatgaattt tatgcggatt tacagaaaaa caaactcaat aaatatctta
      421 ctgttgatga gtatgataat ttagagtgtt ttcttgtaaa gttcttagag aaaagtttaa
      481 tttca
//
LOCUS       DAZGTJ010000375          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000375 DAZGTJ010000000
VERSION     DAZGTJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.381"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>474
                     /locus_tag="ACHK8E_005429"
     CDS             <1..>474
                     /locus_tag="ACHK8E_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HFP0347504.1"
                     /translation="LLRRDSPLNPVRPLPPVGGNPALHGIEPDEQDVTLALGERVGHT
                     IVYGTTRVGKTRLAELLVTQDIRRGDVTIVFDPKGDADLLLRVWAEAHRAGRGDELYI
                     FHLGWPEISARYNAVGRFGRVSEVASRVAGQLSGEGNSAAFREFAWRFVNIVARAL"
BASE COUNT           74 a          121 c          159 g          120 t
ORIGIN      
        1 gcttcttcgt cgtgactcgc cgctgaatcc cgtccgtccc ctgccgccgg tgggaggaaa
       61 tcctgccctg catggtattg agccggatga gcaggatgtg acgctggcac tgggtgaacg
      121 tgtggggcac accattgtct atggcaccac ccgtgtcggg aaaacccggc tggcggagtt
      181 gctggtcaca caggatattc gtcggggaga tgtcaccatt gtctttgatc ccaaagggga
      241 tgcggatctg ttgctgcgtg tctgggctga agcacaccgt gccggcaggg gcgatgagct
      301 ttatatcttt catctgggct ggccggaaat ctctgcgcgt tataacgccg tggggcgttt
      361 tggtcgtgtg tcggaggtgg cttcccgtgt ggccggtcag ttatccggtg aaggtaacag
      421 tgcggccttc cgtgagtttg catggcggtt tgtcaacatt gttgcccgtg cact
//
LOCUS       DAZGTJ010000376          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000376 DAZGTJ010000000
VERSION     DAZGTJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.382"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHK8E_005430"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHK8E_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFP0347505.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZGTJ010000377          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000377 DAZGTJ010000000
VERSION     DAZGTJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.383"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..366
                     /locus_tag="ACHK8E_005431"
     CDS             <1..366
                     /locus_tag="ACHK8E_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0347506.1"
                     /translation="LGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHTDLLVLLRVY
                     LGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRESLTVSLGCY
                     EGLKCAALPSAEDGHYRFE"
BASE COUNT           87 a          124 c          150 g          103 t
ORIGIN      
        1 ctgggtaaaa cgacagggga agcatgcagc cggctcctga tgacactgga aacagcagat
       61 ccggacgagg cggaaggctg gttgcccgga ggtgttctcc ataccgattt gctggtcctg
      121 ctgcgcgttt atctcggcta ccgcagtgac gtccggctgc gccttactgt ccccgtgcgg
      181 ttgttgcctg agccccgcct gggcaaaggc aggcctgttc agctgggccg taccggttta
      241 ctggggctga aagcgggcaa actcagcaat ggccgggaga gtctgacggt cagtctgggt
      301 tgctacgaag ggctgaaatg tgctgcgcta ccgtctgctg aagacgggca ttatcgcttc
      361 gaataatcac ctgttctctc tcaataaagg aacgcctgaa tggtaacagt aatccgctgg
      421 tgggcgccga tgtttattgc ctgtggcctg ctcgcaggct gcgg
//
LOCUS       DAZGTJ010000378          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000378 DAZGTJ010000000
VERSION     DAZGTJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.384"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            230..>463
                     /locus_tag="ACHK8E_005432"
     CDS             230..>463
                     /locus_tag="ACHK8E_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFP0347507.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAALDDGIITDDIKDDAIR"
BASE COUNT          129 a          101 c          114 g          119 t
ORIGIN      
        1 caccggaaag gtggcggggg actcacaacg ataatatctg tctgaaatta ttttgaatgt
       61 acccggtgca ctgtaaaaga aaggcagtgt tccgttcctc ctccctgtca tgaataacct
      121 ggtgtatctc acaatatctg ctgacttgct ccggggctgc ctgaaccaca agattgctgt
      181 agtattgccc ccggagattc agttccagac caggaaaaac aggttaaaaa tgacaagtat
      241 tcaacaaaga gcagaactgc accgccagat atggcaaatt gctaacgatg tcagaggttc
      301 ggtcgatgga tgggatttta agcaatacgt tctgggcgcg cttttctacc gttttatcag
      361 cgaaaatttc tccgggtata tggaagccgg tgatgacagc atctgttatg cggcacttga
      421 tgacggcatt attactgatg acattaaaga cgatgccatc agg
//
LOCUS       DAZGTJ010000379          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000379 DAZGTJ010000000
VERSION     DAZGTJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.385"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..360
                     /gene="ssb"
                     /locus_tag="ACHK8E_005433"
     CDS             <1..360
                     /gene="ssb"
                     /locus_tag="ACHK8E_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0347508.1"
                     /translation="IAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYSTEVVVSVNGT
                     LQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGMPPMDFDDDI
                     PFMGRGYGVERRIIHVI"
BASE COUNT          114 a          104 c          130 g          107 t
ORIGIN      
        1 attgccggtg aataccttcg taaaggttca caggtgtata ttgaaggtca gttaagaacc
       61 cggaagtggt tcgatcagca gaatggtgtt gatcgttatt ccacggaagt tgttgtgagt
      121 gtcaatggta ccctgcagat gctggggtct ccgcgtcagc agcagggtgg tggaaatgtt
      181 tcatctgctc ctgctcaggg aggatgggga caaccaccac aaaatgcaca acaatcaacg
      241 ggaaaaaacg gtcaggcttc ctcaggaagc gggggaatgc cgccgatgga ctttgacgat
      301 gatatcccct ttatggggcg tgggtatggc gttgaacgac ggattatcca tgtcatctga
      361 tgttaaccac ggggcttacc gccccacttt taattcaagg aggcggttgt gaatgaaaac
      421 acacccacta cccccacgcc acatgatgcg gcgtt
//
LOCUS       DAZGTJ010000380          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000380 DAZGTJ010000000
VERSION     DAZGTJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.386"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>451)
                     /locus_tag="ACHK8E_005434"
     CDS             complement(<1..>451)
                     /locus_tag="ACHK8E_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0347509.1"
                     /translation="LWQALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPA
                     PLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEA
                     RQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPDNLP"
BASE COUNT           75 a          162 c          141 g           73 t
ORIGIN      
        1 ggcaggttgt ccggatattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcctct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctcac ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgcct ccggcacccg ctcacaccag
      361 ccgagtaccc accacggccc ctgcggactg ttcgtcgcca gataacgatg cggactcagg
      421 cgaagctctt ccggcagcgc ctgccagagt g
//
LOCUS       DAZGTJ010000381          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000381 DAZGTJ010000000
VERSION     DAZGTJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.387"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..375
                     /locus_tag="ACHK8E_005435"
     CDS             <1..375
                     /locus_tag="ACHK8E_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFP0347510.1"
                     /translation="KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQ
                     REQQREADALIKEENLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTK
                     KQTVFQKISAFIEKFKGVGGHL"
BASE COUNT          146 a           97 c           97 g          101 t
ORIGIN      
        1 aaacgattaa ttcgttcaag tctggggaac cgtgctaaag aaggcctggt ggtcgatttt
       61 attcagcaaa cgaaccttga tgatttgccg gacaaagcca gcatcattga tacgttcttt
      121 acctttgctc aacgcgaaca acaacgtgaa gcagatgcat tgatcaaaga agaaaatctt
      181 aatgaagaag cggccaggcg ctatattcgc acgtcgttaa aacgcgaata cgccaccgaa
      241 aatggcacag aattaaacga gacattacca aaacttagtc cgttaaaccc gcaatataaa
      301 acgaaaaaac agaccgtttt tcagaagatt agcgcattta ttgagaagtt taaaggcgtg
      361 ggggggcatc tttagttaca ggcgcccgga ctcccccttg cctcaataaa caggcatgaa
      421 cactgactgc tattgtaagg c
//
LOCUS       DAZGTJ010000382          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000382 DAZGTJ010000000
VERSION     DAZGTJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.388"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..285
                     /locus_tag="ACHK8E_005436"
     CDS             <1..285
                     /locus_tag="ACHK8E_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347511.1"
                     /translation="RVVLLDAPEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQA
                     GLAEHYRALAHAWSLGLKKYPAVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            282..>441
                     /locus_tag="ACHK8E_005437"
     CDS             282..>441
                     /locus_tag="ACHK8E_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347512.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWL"
BASE COUNT           90 a          110 c          137 g          104 t
ORIGIN      
        1 cgtgttgtcc tgcttgatgc tcctgagcag acgcaggata catttttcgg ggtacttccc
       61 gcagagcctg cagaagcatc tgcccgtgtc atggaacgga tgcagtcgcc tgaatggcag
      121 tcgtttcagg cagggcttgc tgaacactac cgtgcattag cacatgcctg gtcccttgga
      181 ctgaaaaaat atccggctgt ggtttttgat gacagtgaag tggtctacgg aaccacagac
      241 gtggtgctgg cagaacaact gcgtacaggg ggagggcagc catgatttcc tgcacatcac
      301 gtcatcgcgc cggtgttctg ctgttaagta tggcggtatt gccggtggca gaggcatcgc
      361 tgaacaccgc gcagatagtg gccagtgcag cctcacagag ctgtatcagc tggcgggtca
      421 gcgggatttg ctactggctg t
//
LOCUS       DAZGTJ010000383          436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000383 DAZGTJ010000000
VERSION     DAZGTJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.389"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..341
                     /locus_tag="ACHK8E_005438"
     CDS             <1..341
                     /locus_tag="ACHK8E_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP0347513.1"
                     /translation="ELLPPGEQSMLEIWQGKGSGGHSRHMTAARDTLRRPLTMPEQQA
                     DAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGTSPVPGYSTVFPFYSRTQY
                     AMPGERTEAL"
BASE COUNT          105 a          110 c          135 g           86 t
ORIGIN      
        1 aggagcttct gcctccgggt gaacagagta tgctggagat ctggcagggg aaaggaagcg
       61 gaggacattc gcggcacatg acagcggcca gggacaccct gcggcgtccg ctgacaatgc
      121 cggagcagca ggcagatgcg cacggagcca taagctacag ccggacacag gaaagtgaga
      181 tcagtcagca gtttccgcgg ctgcccaatc ctgacatggt gatgtatgtt tttccgcatc
      241 tggcttccgg tacgtcaccg gttccggggt acagcacagt atttcctttt tacagccgga
      301 cgcagtatgc catgccgggt gaacgaacgg aggcgttgtg atgcgatttt ccctgtttac
      361 agacacaaaa aaacgggttt cttccccggt gtcgcagcat gacggagaag cggtatctcc
      421 ggatatgaaa gaaaga
//
LOCUS       DAZGTJ010000384          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000384 DAZGTJ010000000
VERSION     DAZGTJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.390"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>430)
                     /locus_tag="ACHK8E_005439"
     CDS             complement(<1..>430)
                     /locus_tag="ACHK8E_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HFP0347514.1"
                     /translation="SQEERLSLISRANEALSLWDNWLHPFTSGSETGDDPAYDDNFQL
                     MREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRDGERGLSEGLLLLV
                     AMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGV"
BASE COUNT          101 a          128 c          109 g           92 t
ORIGIN      
        1 tccaccccgg gccataacag cagtgcatca gtcacttttg cactgttcag ccactccagc
       61 gcagctttcc gggcggcagg acgccgggga tgacatgcct gtccgtagcg ggtcagcatc
      121 gcaacaagaa gtaataagcc ttcggacaac cctctttcac catcccgggt gagacgggcc
      181 agcacgtacc aggtcacgac acgaatatct ctggcgcatt cgcaaaggca cttctgagcc
      241 agttcgcaca acagcgctga atcagtacct gaaatcttat tgatctcttc acgcatcagc
      301 tgaaaattat catcgtatgc aggatcgtcc ccggtttcag atccggaggt aaatggatgc
      361 agccagttat cccagagcga cagggcctca tttgcgcggg aaatgaggct cagcctttct
      421 tcctggctgg
//
LOCUS       DAZGTJ010000385          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000385 DAZGTJ010000000
VERSION     DAZGTJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.391"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..305
                     /locus_tag="ACHK8E_005440"
     CDS             <1..305
                     /locus_tag="ACHK8E_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347515.1"
                     /translation="KQLFELIVTVFSVFDSHQKEMMLANGDKFIDSLYLAIEENSSRY
                     AKWESDTYEKDDRGFRDSISTSTGKRISVVYRFDAFLNILGKSTGITIDSKLLQGE"
     gene            305..>429
                     /locus_tag="ACHK8E_005441"
     CDS             305..>429
                     /locus_tag="ACHK8E_005441"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0347516.1"
                     /translation="MKKITSLTLKNFKAYKEQSFDFSNLTVFCGNNSVGKSTAIQA"
BASE COUNT          151 a           47 c           84 g          147 t
ORIGIN      
        1 ataaacaact ctttgagtta attgttactg ttttctctgt atttgactca catcaaaagg
       61 agatgatgtt agctaatggt gataaattta ttgatagctt atacttggct attgaggaga
      121 atagtagtcg atatgctaag tgggagtctg atacatatga aaaggacgat agaggattta
      181 gagactcaat atctacttcg acaggtaaaa gaatatctgt tgtatatcga tttgatgctt
      241 ttttaaatat ccttggaaaa agcacaggaa taactattga tagtaaatta ttacaggggg
      301 agtgatgaaa aaaattacgt ctttaacatt aaaaaatttc aaagcttata aagaacagag
      361 ttttgatttt agtaatttaa ctgtattttg cggtaataat tctgttggta aaagtacggc
      421 tattcaagc
//
LOCUS       DAZGTJ010000386          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000386 DAZGTJ010000000
VERSION     DAZGTJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.392"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>428
                     /locus_tag="ACHK8E_005442"
     CDS             <1..>428
                     /locus_tag="ACHK8E_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0347517.1"
                     /translation="LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVS
                     GDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESSRAYDELDRLVSETSRSGETVRY
                     RYDDVHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGY"
BASE COUNT          101 a          112 c          157 g           58 t
ORIGIN      
        1 gctgaagcgg gtggtgaaaa aagaactggc ggacggcagc gtcacgcaca gcgggtatga
       61 cgcggcagga aggctcacgg cacagacgga cgcggcggga cggcggacag agtacggcct
      121 gaatgtggtg tccggcgata tcacggatat caccacgccg gacgggcggg agacgaaatt
      181 ttactataac gacgggaacc agttaacggc ggtggtgtac ccggacgggc tggagagcag
      241 ccgggcatat gatgagctgg acagactggt atcggagaca tcgcgcagcg gggagacagt
      301 acgctaccgc tacgatgacg tgcacagtga gttaccggcg acgacaacgg atgcgacggg
      361 cagcacccgg cagatgacct ggagccgcta cgggcagttg ctggccttca ccgactgctc
      421 gggctacc
//
LOCUS       DAZGTJ010000387          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000387 DAZGTJ010000000
VERSION     DAZGTJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.393"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>426
                     /locus_tag="ACHK8E_005443"
     CDS             <1..>426
                     /locus_tag="ACHK8E_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFP0347518.1"
                     /translation="PASIESEKEKKDFVKLFGEYLRAENVLQNYDEFATLKALQQIDL
                     SDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDWQHREKEADKKE
                     KSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKS"
BASE COUNT          145 a           86 c           94 g          101 t
ORIGIN      
        1 ccctgccagt attgaaagcg aaaaagagaa aaaagacttc gttaaactgt ttggtgaata
       61 cctgcgtgcc gagaacgtcc tgcaaaacta tgatgaattt gccacgctga aagccctgca
      121 acaaatcgat ctcagcgatc ctgttgcggt agaaaaattc aaagcagaac attatgtgga
      181 tgatgaaaag ttcgctgaat tgcaaatgat tcgtctccct gctgaacgca agattcagga
      241 ttatcgttct gcctataacg atattcgtga ctggcagcac cgcgaaaaag aagctgacaa
      301 aaaagaaaaa tcaaccactg actgggatga cgtggttttt gaggtcgatt tgctgaagtc
      361 tcaggaaata aacctggatt atatccttgg cctgattttc gaacacagca gacaaaacaa
      421 aagtaa
//
LOCUS       DAZGTJ010000388          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000388 DAZGTJ010000000
VERSION     DAZGTJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.394"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..182
                     /locus_tag="ACHK8E_005444"
     CDS             <1..182
                     /locus_tag="ACHK8E_005444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347519.1"
                     /translation="RLPEWPEERLHDLLPFSAYRFAELTDVTVIPPNAGKRVMEVVSS
                     TPMNDTASEVKIPTQ"
BASE COUNT          140 a          106 c           73 g          104 t
ORIGIN      
        1 cacgtctgcc tgaatggccg gaagagcgct tgcacgactt actaccgttc tcagcctacc
       61 gattcgcaga attaactgac gttacggtca taccacctaa tgcagggaaa cgagtcatgg
      121 aggtcgtcag ctctactccc atgaatgaca cagcctctga agtaaaaata ccgacgcagt
      181 gaaaccagcc ttaagctttc caaaacacaa ttacaacata gttgaacaaa gttcattcaa
      241 acaggttctt ttaataacat ttagtcaaat aaaataacaa taaaacgatt cagcaatcat
      301 ttttcataac aaaaacaaat tataaccccc ttccctgttt gtggacgaga ctcgttctct
      361 ccctgcgaga caagcgtaaa ttggtcacac cgacggtagt tgcctgaaaa ttacaaattc
      421 tcg
//
LOCUS       DAZGTJ010000389          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000389 DAZGTJ010000000
VERSION     DAZGTJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.395"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>422)
                     /gene="tli1"
                     /locus_tag="ACHK8E_005445"
     CDS             complement(<1..>422)
                     /gene="tli1"
                     /locus_tag="ACHK8E_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFP0347520.1"
                     /translation="FLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRW
                     NDHLGATESYYNKGKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWN
                     ETYYRSTMIIGLAPEGKVRVWLGDRGNPVVPQLDAKITT"
BASE COUNT          115 a          111 c           80 g          116 t
ORIGIN      
        1 gtagtaattt tagcatccaa ctgaggtacc accggattac cgcgatctcc cagccagact
       61 cttaccttcc cttcaggtgc cagaccaata atcatcgttg aacgataata ggtttcgttc
      121 cagcggttgt ctgtgtaagg cgtaaccatc tgctcccagg tccctttttt aaaaaaaatc
      181 agagtttcat aactcttttt atcaattatt gaatcccagc aaaaacgtaa catcagaggt
      241 aatgctttcc ccttgttata atacgactca gtcgcgccca gatggtcatt ccagcgccct
      301 acactgacct gactcggtct tgtggtatca agatactgat aagtcgtctt ataaccttcc
      361 ccgtccacca tcaatgcctg agtggccagc gcaggcagag cctttggata aataaagaga
      421 aa
//
LOCUS       DAZGTJ010000390          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000390 DAZGTJ010000000
VERSION     DAZGTJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.396"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..323
                     /locus_tag="ACHK8E_005446"
                     /pseudo
     CDS             1..323
                     /locus_tag="ACHK8E_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          175 a           74 c           58 g          108 t
ORIGIN      
        1 agacgaacaa tatcttgaca aacaaatcga aggtttattt tcaacattat taaccattaa
       61 tgacaacttt ttacgagcaa aagcggaaat tgcttcgaca ataattaaat tcttagaggc
      121 caggataacc aatatttcat acaatgacat attaaaatat cagcaagaat tcaaaaaaca
      181 atgttataag caagttaaag cctttacgac actctcccga tacaataaaa ttcaaacatg
      241 ggcagaaatg tctgaatatc aattcgaagt atttcaatac gagacgctta acccaaaaaa
      301 aatgtcatac gcaccttatt taaagagatc gctacctgat gaaaaaacta taaattatgg
      361 ggtggaaata gaaacaccaa ctggcaaaag aattcgctta ttaaatcatt accag
//
LOCUS       DAZGTJ010000391          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000391 DAZGTJ010000000
VERSION     DAZGTJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.397"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHK8E_005447"
     CDS             complement(<1..200)
                     /locus_tag="ACHK8E_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0347521.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDD"
     gene            complement(234..>413)
                     /locus_tag="ACHK8E_005448"
     CDS             complement(234..>413)
                     /locus_tag="ACHK8E_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0347522.1"
                     /translation="TLEDWEPALLFEVRARRLKLLRMKAGRSESDKARLMPEMDTLLA
                     GLIAIDPARAMVLCG"
BASE COUNT          101 a          130 c          101 g           81 t
ORIGIN      
        1 tcatcatcca gcttttcacg caaacgtccc atcaggaagg caaagccttc aaacagacgt
       61 tccacaccgg gatcgcaggc gccaggctta tccagattca gcattgctgc ccgatccgga
      121 tgagcacggg caaactcttt accggcttcc cgcagatagc gcatctctgc ttcgtaatag
      181 cgcagggtta aatcatccat gcacggcatt cctgaaaatg attaaagaga acatcagcca
      241 cagagcacca tggcacgggc cgggtcaatg gcgatcagcc ccgccagcag cgtatccatc
      301 tcaggcatca gacgggcttt atcagattcg ctgcgaccgg ctttcatccg tagcagcttc
      361 aggcgtcggg cccggacttc aaacagcaaa gcgggctccc agtcttccag cgt
//
LOCUS       DAZGTJ010000392          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000392 DAZGTJ010000000
VERSION     DAZGTJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.398"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..342
                     /locus_tag="ACHK8E_005449"
     CDS             <1..342
                     /locus_tag="ACHK8E_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFP0347523.1"
                     /translation="CRTNYKIEKDVITNAGPQNFLTLFKNAAFVVTTSFHGTVFSIIN
                     QVPFFTLEFGNEIDLRSRSLLEMLNLNERMISDDANLNYEKLFLEFDEAHSILESKRQ
                     DSLSFIERALS"
BASE COUNT          145 a           59 c           78 g          131 t
ORIGIN      
        1 tgtcgaacaa attacaaaat tgagaaggat gtaataacaa atgcaggacc acaaaatttc
       61 ttaactttat ttaaaaatgc agctttcgtg gtgaccactt cttttcacgg aactgtattc
      121 tcaattataa atcaggtgcc attctttact ttggaatttg gtaacgagat agacttaaga
      181 agccgttcac ttcttgaaat gcttaatttg aatgaacgaa tgatcagtga cgatgcaaat
      241 ttgaattatg agaagctttt cttggaattt gacgaggctc attcaatatt agaaagtaaa
      301 aggcaggatt ctttaagttt cattgagaga gctctgagtt aaataaaatg actaaagttt
      361 taatagtatt ccatgacggt gataaaaaaa gtggtgcaac agcctcaatg tta
//
LOCUS       DAZGTJ010000393          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000393 DAZGTJ010000000
VERSION     DAZGTJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.399"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>404)
                     /locus_tag="ACHK8E_005450"
     CDS             complement(<1..>404)
                     /locus_tag="ACHK8E_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0347524.1"
                     /translation="KHIRSTQLGRELVAALPDSLTNPGMTALWEQALDDVARGKISLE
                     AFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLCNGPTVKRKGKSGFFWGCSRYPDC
                     RGIAGEQGNSRGRKRKINFSHKQKSGDVGDKL"
BASE COUNT           88 a          120 c           83 g          113 t
ORIGIN      
        1 ttcagtttgt ctccgacatc accagatttt tgtttatgag aaaaattaat ttttcttttc
       61 ctgcccctgc tgtttccctg ctcaccagca attcccctgc agtcgggata acgggagcat
      121 ccccagaaaa aaccgctttt tcccttccgt ttcaccgtcg gaccgttaca gagtggacat
      181 gaaggacctg attccactga cagattaacc gtctggttac gtccggataa caccaggtgc
      241 ctgatccact gatgctgttt ctccataaac gcctccagtg agattttccc ccgggcaaca
      301 tcatccagcg cctgttccca taaagccgtc atacccggat ttgtcaggga gtccggcaat
      361 gccgccacca gttctcttcc cagttgcgtg gaacggatat gttt
//
LOCUS       DAZGTJ010000394          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000394 DAZGTJ010000000
VERSION     DAZGTJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.400"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>402)
                     /locus_tag="ACHK8E_005451"
     CDS             complement(<1..>402)
                     /locus_tag="ACHK8E_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFP0347525.1"
                     /translation="ELIQDLQNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWR
                     RFTEQYLDNPGDGILDKTRKIHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQF
                     EQAGSHSNRYDVTILVNGLPLVQIELKKRGVA"
BASE COUNT          104 a          103 c           84 g          111 t
ORIGIN      
        1 cgccacgcca cgctttttca gttcgatttg caccagcggc aaaccattaa ccaggatagt
       61 gacgtcataa cggttactgt gagaacctgc ctgttcaaac tgccggataa tctgcacttt
      121 gttgcgcatg agattctttt tatctatcag atagatattc tcaagacgcc cgttgtcaaa
      181 aataaagtca cagatatagt cgatatggat tttacgggtc ttatccagaa tgccatcgcc
      241 agggttgtcc agatactgct ccgtgaaacg ccgccattcg ctgtcattaa acaccacgcc
      301 attgaggctc tgaagctgtt cccggacatt agccagcatc gccgactgtg attttacgga
      361 aataaattca tagccctgat tctgcaggtc ctgaatcagt tc
//
LOCUS       DAZGTJ010000395          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000395 DAZGTJ010000000
VERSION     DAZGTJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.401"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          124 a           91 c          110 g           77 t
ORIGIN      
        1 gacgaaaacg gaaaaatcag gaaaaccaga actggcgcgg cctggcgggg agtggcagca
       61 aaaatcactt ttgcacactt ttgcacaact gctccgaaaa aggatggcga aggggaagga
      121 aaacagccgg atcatccgtt gcacgtaacg gacgtaaaag gtaaattacg cgcgggtgcc
      181 tttcggctga cggctggaag gaatacctga aggccggatg tgaaaaggcc ccgagtcaac
      241 taacgttaac ccgaggccct gacacttcct acctgacaag tggaaggtta gcgcctccct
      301 gtaaaaggag tcaagcgcta tgccgcaaaa aacgatcata gtgagcattt tatgcataac
      361 tattttgatg attgtctgga tgatacacaa gtcaccgtgt ga
//
LOCUS       DAZGTJ010000396          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000396 DAZGTJ010000000
VERSION     DAZGTJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.402"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          120 a           73 c           66 g          143 t
ORIGIN      
        1 gctcgatacc aatgattttt atttcagaca tgatatcctt cttacatttc attgattaaa
       61 gtttatccat catggtatga tctgcttcta ccgtgaagga ggggctgaca ctcaatggac
      121 caaaaactca cgttaaacaa ttgtagcaaa ggaaagttaa aggatcctgc gaggatcctt
      181 tgataggtgt taaaattgca ttcttattac catataagat cagttatctc tcagtagctg
      241 tgagcatatt aaatttatat tgacacttca tccgtactca ttactttttc cttattgata
      301 actcttgtaa ttcctaaatt atttggaata tgtatgccat cttgagttat aagtaactct
      361 gttccaactc gtttcgttgc agcagaataa ttcaatgggt ac
//
LOCUS       DAZGTJ010000397          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000397 DAZGTJ010000000
VERSION     DAZGTJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.403"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..319)
                     /gene="subB"
                     /locus_tag="ACHK8E_005452"
     CDS             complement(<1..319)
                     /gene="subB"
                     /locus_tag="ACHK8E_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFP0347526.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVW"
BASE COUNT          128 a          101 c           66 g          106 t
ORIGIN      
        1 tccatacccc gggttcataa taaatcctga ccaactgacc tgttgtgtaa aaatatagtg
       61 cctgattata taatgtggaa aacgaaggcc cccagacaga tgaattcttc atcgagcagg
      121 ctcttatgga agaaccacca ggttgtttcc cttcaataca aaaataaggg ttattatcta
      181 tttgccctgt atgaaactga tcaataacaa cgcctgaaaa catgccatcc cgggcatcgc
      241 cagtccactc agccatggca ggattaaggg ataaacatcc cataacacct gcacatacaa
      301 aaaacctctt aatcgtcata ttcacctccg gaattttaca gttcttcact catccttccc
      361 tgacggacag gaatataatt tttcttgcag aacatgctaa t
//
LOCUS       DAZGTJ010000398          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000398 DAZGTJ010000000
VERSION     DAZGTJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.404"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>400
                     /locus_tag="ACHK8E_005453"
     CDS             <1..>400
                     /locus_tag="ACHK8E_005453"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0347527.1"
                     /translation="DELKSIPDVIIKHLGQNRGIATAQNVGLRISLSLCSKYALLLDQ
                     DSEIETDLVSKLLDGYITLEQKGIKLAAVGPRPFDLFENKKMKANIQRDIQIFEGFSL
                     TKQIIASGKLINLEVLDQVGFMEDDLFIDGV"
BASE COUNT          138 a           61 c           76 g          125 t
ORIGIN      
        1 tagacgaatt aaaaagtatt ccagatgtta taattaagca ccttggtcaa aaccgtggta
       61 ttgcaacggc acaaaatgtc gggctaagaa tttctctatc tttatgctca aagtatgcgc
      121 tattactgga tcaagattca gaaattgaaa cagatttagt gtcaaaactt ctcgacggtt
      181 acattacatt agaacaaaaa ggcatcaaac ttgccgctgt tggtccccga ccgtttgatt
      241 tattcgaaaa caaaaaaatg aaagcaaaca ttcaaagaga tattcaaata tttgagggtt
      301 tttctttgac gaaacagata atcgcttcag gtaagctaat taatttggaa gttttagatc
      361 aagtcggttt tatggaagat gatttattta ttgatggtgt
//
LOCUS       DAZGTJ010000399          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000399 DAZGTJ010000000
VERSION     DAZGTJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.405"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>397
                     /locus_tag="ACHK8E_005454"
     CDS             <1..>397
                     /locus_tag="ACHK8E_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0347528.1"
                     /translation="HGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQ
                     VIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGWQVIKE
                     GGTAAHTTINQKGKLQVNAGGKASDVTQNT"
BASE COUNT          110 a          100 c          127 g           60 t
ORIGIN      
        1 acatggcgga ctggcaaccg gaaccatcgt caacaccggt gcagaaggtg gtccggagtc
       61 tgaaaatgtg tccagcggtc agatggtcgg agggacggct gaatccacca ccatcaacaa
      121 aaatggccgg caggttatct ggtcttcggg gatggcacgg gacaccctca tttacgccgg
      181 tggtgaccag acggtacacg gagaggcaca taacacccgg ctggaggggg gtaaccagta
      241 tgtacacaac ggtggcacgg caacagagac gctgataaac cgtgatggct ggcaggtgat
      301 taaggaagga ggaactgccg cgcataccac catcaaccag aaaggaaagc tgcaggtgaa
      361 tgccggcggt aaagcgtctg atgtcaccca gaacacg
//
LOCUS       DAZGTJ010000400          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000400 DAZGTJ010000000
VERSION     DAZGTJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.406"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>397
                     /locus_tag="ACHK8E_005455"
     CDS             <1..>397
                     /locus_tag="ACHK8E_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP0347529.1"
                     /translation="LMLARPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFY
                     AQNLPAHPEWKIKPDDELISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLAD
                     MTGDTDASTVFSTDEVVPGMFTRQAWEEQV"
BASE COUNT          101 a           96 c          117 g           83 t
ORIGIN      
        1 atctgatgct ggcgcgcccg gacaaagcgg acgccggctg gctggcaaaa aatgcgctga
       61 aagccttacc gaaacgccgg aatgtgccgg atggcatatg gcagaacgtc gcaccgaaac
      121 tgctggggtt ttatgcgcaa aaccttccgg ctcatccgga atggaaaatt aagcctgacg
      181 atgaattaat cagtacggtc cgtcagattc tgcttaagca aattggccag cggaatgctg
      241 agtccgggtt atatcaggag atgcttaagc gcgtggccag taactggcct gatttaacac
      301 ttgcggatat gaccggtgac accgatgcct caacggtatt cagcactgac gaagtcgttc
      361 cgggaatgtt tacgcgtcag gcctgggaag agcaggt
//
LOCUS       DAZGTJ010000401          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000401 DAZGTJ010000000
VERSION     DAZGTJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.407"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>395
                     /locus_tag="ACHK8E_005456"
     CDS             <1..>395
                     /locus_tag="ACHK8E_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0347530.1"
                     /translation="IFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLR
                     EMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAP
                     GLFIDGYTSAAASTVPSLSRTLIYDYEQNP"
BASE COUNT           93 a           97 c          108 g           97 t
ORIGIN      
        1 attttctggc ggacacacca ccgcggtcac cgtaactggc tggcactgct gctgttcgta
       61 ttatgctctg taaacagctg gccgttgcgg atggttaaag gaaccgttgt ggggacaact
      121 gacacattgc gtgaaatgca gcgttatgaa caactgagtc agcacagggc tgacagctgg
      181 aaaatcctgc cgggagcacc gttgtatgac acgattgtga ttgtcactgg tgagagtgtg
      241 cgcagggatt atatgtcggt atatggttat cccgtaccaa ccacaccgtg gctgaatacg
      301 gctcccggtt tatttattga cggctataca tcggcagcag ccagtaccgt accttccctg
      361 agccggacac tgatttatga ctatgagcag aaccc
//
LOCUS       DAZGTJ010000402          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000402 DAZGTJ010000000
VERSION     DAZGTJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.408"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>394
                     /locus_tag="ACHK8E_005457"
     CDS             <1..>394
                     /locus_tag="ACHK8E_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347531.1"
                     /translation="EKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYGVL
                     MLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVLTA
                     SWKEQLENLPEMLEQIESAREKLSLRLDT"
BASE COUNT          100 a           90 c          129 g           75 t
ORIGIN      
        1 gtgagaaaca gccgcagata ccggagggtg cgtctggtat gtcgccggag cgaagaaaaa
       61 gtcgtttccc cggcgtgttg atactgtctg tcactgtggg gctggtcatg ggggcagtgg
      121 tatatggcgt actgatgctt atgaccggtc ttgacagccg gatcacgacc ctggaaggcc
      181 agagaagaga aatgctttct gcgtcagaac tcgcgcccct gagaatgaaa ctgacagaga
      241 cggagaagca actgaaggat atggaaaagc gcctggcagt cctggagcag cagcccgtgc
      301 tgacagcatc ctggaaggaa caactggaga atctgccgga gatgctggag cagattgagt
      361 ctgcccggga aaagctttcc ctgagactgg atac
//
LOCUS       DAZGTJ010000403          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000403 DAZGTJ010000000
VERSION     DAZGTJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.409"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..237
                     /locus_tag="ACHK8E_005458"
     CDS             <1..237
                     /locus_tag="ACHK8E_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0347532.1"
                     /translation="RTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPYVTIDVSSKS
                     HPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
BASE COUNT           95 a           88 c           97 g          100 t
ORIGIN      
        1 cgcacagtag tgttccatga cacgagtgtg gatgagtact ttaaaatagg ttctaccatc
       61 aaaacagatc gcgagattga actggatggt gtgacgtatc cgtatgtgac gattgacgtt
      121 tcctccaaat cgcatccgtt ctataccggt aaactcagaa cggtggcatc agaaggcaat
      181 gttgcccgct ttactcagcg ttttggtcgt ttcgttggga ctcagaaggg agaatgatga
      241 gagtgagagt cttaacaggc tccatgcgtg gtggatttgc tcctatattt ccagacatct
      301 ggtatcactt aacccattac tgcccgccgc cgcagatatt cccgtggaga gcgataaccc
      361 agcgcactat gtggatgcca
//
LOCUS       DAZGTJ010000404          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000404 DAZGTJ010000000
VERSION     DAZGTJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.410"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>378
                     /locus_tag="ACHK8E_005459"
     CDS             <1..>378
                     /locus_tag="ACHK8E_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0347533.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAGR"
BASE COUNT           93 a          103 c          131 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggccgg
//
LOCUS       DAZGTJ010000405          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000405 DAZGTJ010000000
VERSION     DAZGTJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.411"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>374
                     /locus_tag="ACHK8E_005460"
     CDS             <1..>374
                     /locus_tag="ACHK8E_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0347534.1"
                     /translation="LHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQD
                     FEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASA
                     HMPFIGSVGPAFFRKDTMEEVAA"
BASE COUNT           77 a           81 c          116 g          100 t
ORIGIN      
        1 cttcatcatc cggaatttca gaaagtggag tccctgtggc ggggcgtgaa gtctctggtg
       61 gataaaacgg acttccgccg taatgtgaaa attgaactgc tggatctgtc caaagacgat
      121 ctgcgtcagg attttgagga tgcgccggaa attattcaga gtggtcttta tctgcagact
      181 tatgtggcgg agtacgatac gccgggtggt gaacccattg cagccctgat ttctgcctgg
      241 gagtttgatg cgtctgcgca ggatgtggcg ctgctgaaaa atatttcccg ggtggctgcg
      301 tccgcgcata tgccatttat tggttcagtt ggcccggctt tcttccggaa ggatacgatg
      361 gaagaggtgg ccgc
//
LOCUS       DAZGTJ010000406          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000406 DAZGTJ010000000
VERSION     DAZGTJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.412"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>372
                     /locus_tag="ACHK8E_005461"
     CDS             <1..>372
                     /locus_tag="ACHK8E_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347535.1"
                     /translation="NALGATAEANGDMATAVGFNALANASNALAVGSQSSAGGEESLA
                     IGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDKAATPVSGATIKLKNGGTIEYR
                     GFAGDKAASVVSVGDAGKERQ"
BASE COUNT           84 a           79 c          129 g           80 t
ORIGIN      
        1 taatgcactg ggagccacgg cggaagctaa cggggatatg gcgactgcgg ttggttttaa
       61 tgcgctggct aatgccagta atgcgctggc tgttggtagt cagtcctctg ccggcggaga
      121 ggaatccctg gctataggga gtcagagtac gacgggcaaa aagtccacgg ttgctctggg
      181 gcagggagct acagcctctg ccgcggaggg gaacgtggcc atcggtgctg attcagtgga
      241 caaggcagca actccggttt ccggcgctac cattaagtta aagaatggcg ggactattga
      301 atacaggggc tttgccggag ataaggctgc atcagtagta tctgttggtg atgcgggtaa
      361 agagcgtcag at
//
LOCUS       DAZGTJ010000407          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000407 DAZGTJ010000000
VERSION     DAZGTJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.413"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..231
                     /locus_tag="ACHK8E_005462"
     CDS             <1..231
                     /locus_tag="ACHK8E_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347536.1"
                     /translation="KSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLG
                     EGESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
BASE COUNT           71 a           95 c          127 g           75 t
ORIGIN      
        1 aaatcccgcg cagttcatac agttcggcag gctccttttg tgctgaccgg gacagagctg
       61 cgcggtggca gtacccgtgc agccgtggct ccccgggggt ataccagcct ctctcaggtg
      121 gctctgctgg gtgaaggaga gagtgtggcc gggtggacgc ttgtgtatgc cggccatggt
      181 gaagcaacgt tccgggtgaa tggtcgtcag acggtgctca gggtggagta gatgatgaag
      241 cgaaaaatac tgtatctgct ggcactgtgc cccgttctgg ctcagtccgc cgggcagacg
      301 aatatgtcgt cagaggcgca acggacgacc ggtacggtgg tgacgcagag taccacatcc
      361 ggacttca
//
LOCUS       DAZGTJ010000408          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000408 DAZGTJ010000000
VERSION     DAZGTJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.414"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..320
                     /locus_tag="ACHK8E_005463"
     CDS             <1..320
                     /locus_tag="ACHK8E_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP0347537.1"
                     /translation="KTGALTGDTGGRLALFVKAKECGTCDARLASVLATGRQVDIYLV
                     DSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRFGEGKMPVVLQQGADGWRV
                     AAF"
BASE COUNT           72 a           84 c          130 g           80 t
ORIGIN      
        1 gaaaaacagg agcacttacc ggagatacgg ggggacggct ggcgctgttt gtgaaggcga
       61 aggaatgtgg cacctgtgat gcccgtcttg cctccgtact ggccacggga cggcaggttg
      121 atatctacct tgtcgacagt cagggaaagg atgagattct gcgtcagtgg gcgcatgcgc
      181 acagcatacc tgtggagaaa gttcgcagcc gtcatatcac cctgaatcat gatgccggac
      241 gctggctgcg ttttggtgaa ggaaaaatgc cggtggtact gcagcagggg gctgacggat
      301 ggcgcgtggc ggcattctga cggttctgct gatggtggcc tgcagtggtg tctgtcgtgc
      361 tgccgt
//
LOCUS       DAZGTJ010000409          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000409 DAZGTJ010000000
VERSION     DAZGTJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.415"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>363
                     /locus_tag="ACHK8E_005464"
     CDS             <1..>363
                     /locus_tag="ACHK8E_005464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347538.1"
                     /translation="PVSVMAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTP
                     SIMTFPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPTILISLDKELNNNQHI
                     EITRYKVDVDNDNILYEVV"
BASE COUNT          130 a           57 c           66 g          110 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa ctatattaat atctcttgat aaggagctga ataataatca
      301 acatattgaa attacacgtt ataaagttga tgttgataac gataatatat tgtatgaagt
      361 agt
//
LOCUS       DAZGTJ010000410          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000410 DAZGTJ010000000
VERSION     DAZGTJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.416"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>356
                     /locus_tag="ACHK8E_005465"
     CDS             <1..>356
                     /locus_tag="ACHK8E_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFP0347539.1"
                     /translation="TKCFDIIFLGSDQIWNANYTNGVDPNYYGQGPYCKAHKLVSYAA
                     SMGKLCLGKYEEQAFLSLINNIQQIGVRENYLKTYIEEKTDLKCDVNLDPTLLLTKSD
                     WDKLAAPNDTQERYLL"
BASE COUNT          125 a           53 c           69 g          109 t
ORIGIN      
        1 tactaaatgt ttcgatatta tctttcttgg tagtgatcag atatggaatg caaattatac
       61 taacggagta gaccctaact attatggaca aggaccttat tgtaaagcgc acaaattagt
      121 ttcttatgcg gcaagtatgg gtaagctgtg tttgggaaaa tatgaggaac aagcattctt
      181 atctttgata aataatatac agcagattgg tgttagagaa aattatttaa agacgtacat
      241 agaagaaaaa accgatttaa aatgtgatgt taatcttgat ccgactcttc tgcttactaa
      301 aagcgattgg gataaactgg cagcacctaa cgatacacaa gaacgctatc ttctta
//
LOCUS       DAZGTJ010000411          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000411 DAZGTJ010000000
VERSION     DAZGTJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.denovo.417"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>344)
                     /locus_tag="ACHK8E_005466"
     CDS             complement(<1..>344)
                     /locus_tag="ACHK8E_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0347540.1"
                     /translation="QRAEEARTSSLSSSDRPRPLSVSAFPDTLPGTEYGPDRGIRLSA
                     VWLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRY
                     AGRPEMRYRYDD"
BASE COUNT           60 a          108 c          117 g           59 t
ORIGIN      
        1 catcgtcgta gcggtagcgc atctccggcc ttcccgcgta acggtgcgcc accatccggc
       61 ccgggtgctg cgcgtcatac gtgaaagcgc gcacctgcgt attgctgcgg tcatataccg
      121 ccagcagttc accggcttcc gtatacgtgt accgcaccag tggcgcaccg ggcaggctct
      181 ccgggtatgc cgggtcgtgc atcagccaca ccgccgaaag gcggataccc ctgtcggggc
      241 cgtattcggt accgggcagt gtgtcgggga acgctgagac tgagagaggg cggggacggt
      301 cagaagaaga tagcgaagag gtgcgggcct cttccgcacg ctgc
//
LOCUS       DAZGTJ010000412         5036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.guided.192, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000412 DAZGTJ010000000
VERSION     DAZGTJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.guided.192"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            265..2073
                     /gene="frdA"
                     /locus_tag="ACHK8E_005467"
     CDS             265..2073
                     /gene="frdA"
                     /locus_tag="ACHK8E_005467"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0347541.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2066..2800
                     /gene="frdB"
                     /locus_tag="ACHK8E_005468"
     CDS             2066..2800
                     /gene="frdB"
                     /locus_tag="ACHK8E_005468"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0347542.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2811..3206
                     /gene="frdC"
                     /locus_tag="ACHK8E_005469"
     CDS             2811..3206
                     /gene="frdC"
                     /locus_tag="ACHK8E_005469"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0347543.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3217..3576
                     /gene="frdD"
                     /locus_tag="ACHK8E_005470"
     CDS             3217..3576
                     /gene="frdD"
                     /locus_tag="ACHK8E_005470"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0347544.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3639..4772
                     /gene="ampC"
                     /locus_tag="ACHK8E_005471"
     CDS             3639..4772
                     /gene="ampC"
                     /locus_tag="ACHK8E_005471"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0347545.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4861..>5036
                     /gene="blc"
                     /locus_tag="ACHK8E_005472"
     CDS             4861..>5036
                     /gene="blc"
                     /locus_tag="ACHK8E_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0347546.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLE"
BASE COUNT         1233 a         1309 c         1341 g         1153 t
ORIGIN      
        1 aattttacgc aggaatcaaa caagggtggg cagtgactaa aaaaagcacg atctgatggt
       61 ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc
      121 gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag
      181 tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg
      241 ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc
      301 gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa
      361 atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc
      421 tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg
      481 ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgccccacc
      541 gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc
      601 aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc
      661 ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt
      721 tttgacgaac atttcgtact ggatattctg gttgatgatg gtcatgttcg cggcctggta
      781 gcaatgaaca tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct
      841 accggcggtg cgggtcgcgt ttatcgttac aataccaatg gcggcatcgt taccggtgac
      901 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
      961 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggc
     1021 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
     1081 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
     1141 caggccttct ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg tggcgatgtg
     1201 gtttatctcg acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
     1261 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
     1321 ccgaccgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
     1381 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
     1441 ggttctaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
     1501 gagcgtgcag caactgccgg taatggcaac gaagcggtaa ttgaagcgca ggcagctggc
     1561 gttgaacaac gtttgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc
     1621 cgcgacgaaa tgggcctggc tatggaagaa ggctgcggta tctaccgtac gccggaactg
     1681 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaaacg cgtgcgcatc
     1741 accgacactt ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt
     1801 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
     1861 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc
     1921 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
     1981 acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa
     2041 gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt
     2101 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
     2161 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
     2221 gagctaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
     2281 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
     2341 agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga
     2401 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
     2461 taacatccag accccggcgc agatggccaa gtatcaccag ttctccggtt gcatcaactg
     2521 tggtctgtgc tacgccgcgt gtccgcagtt tggcctgaac ccagagttca tcggtccggc
     2581 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
     2641 tatggcgcag ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctactgctc
     2701 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
     2761 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
     2821 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
     2881 gcttctacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
     2941 ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
     3001 tacaaaaccc ggttatcgtg atcattaacc tgatcactct ggcggcagcc ctgctgcaca
     3061 ccaaaacctg gtttgagctg gcaccaaaag cggccaatat cattgtaaaa gacgaaaaaa
     3121 tgggaccaga gccaattatc aaaagcctct gggcggtaac tgtggttgcc accatcgtaa
     3181 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
     3241 tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
     3301 gcgccggtga tgatcctgct ggttggtatt ctgctgccac tggggctgtt tccgggtgac
     3361 gcactgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
     3421 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
     3481 gatctgaaaa tccacgtacc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
     3541 acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc
     3601 cgcctatggc gggccgtttt gtatggaaac cagaccctat gttcaaaatg acgctctgcg
     3661 ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg
     3721 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtata gcggtggcgg
     3781 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
     3841 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
     3901 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcgacaaaat
     3961 actggcctga acttaccgct aaacaatgga atgggatcac actattacat ctcgcgacct
     4021 acaccgctgg cggcctgcca ttgcaggtgc cggatgacgt taaatcctca agcgacttgc
     4081 tgcgcttcta tcaaaactgg caacctgcat gggctccagg aacacaacgt ctgtatgcca
     4141 acgccagtat tgggttgttc ggtgcactgg cggtgaaacc atccggtttg agctttgaac
     4201 aggcgatgca aactcgtgtt ttccagccac tcaaactcac tcatacgtgg attaatgtgc
     4261 cgtccgcaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt
     4321 cgccaggggc gttagatgct gaggcttatg gtgtgaagtc gaccattgaa gatatggccc
     4381 gctgggtgca aagcaattta aaaccccttg atatcactga gaaaacgctt caacaaggga
     4441 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     4501 aaatgctgga ctggccggtc aatcctgaca tcatcattaa cggcagtgac aataaaattg
     4561 cactggcagc acgccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat
     4621 gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa
     4681 aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     4741 ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa tttttggacc
     4801 ttttctccgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt
     4861 atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt
     4921 tctcctacgc cgccccgtgg cgtgaccgta gtgaataatt tcgatgctaa acgctatctc
     4981 ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaa
//
LOCUS       DAZGTJ010000413         3524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.guided.211, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000413 DAZGTJ010000000
VERSION     DAZGTJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.guided.211"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..169
                     /gene="prfH"
                     /locus_tag="ACHK8E_005473"
     CDS             <1..169
                     /gene="prfH"
                     /locus_tag="ACHK8E_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0347547.1"
                     /translation="GHANKRLARLLIAWKLEQQQQENSAALKSQRRMFHHQIERGNPR
                     RTFTGMAFIEG"
     gene            complement(226..1683)
                     /gene="pepD"
                     /locus_tag="ACHK8E_005474"
     CDS             complement(226..1683)
                     /gene="pepD"
                     /locus_tag="ACHK8E_005474"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0347548.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1944..2402
                     /gene="gpt"
                     /locus_tag="ACHK8E_005475"
     CDS             1944..2402
                     /gene="gpt"
                     /locus_tag="ACHK8E_005475"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0347549.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2494..>3524
                     /gene="frsA"
                     /locus_tag="ACHK8E_005476"
     CDS             2494..>3524
                     /gene="frsA"
                     /locus_tag="ACHK8E_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0347550.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYS"
BASE COUNT          821 a          897 c          929 g          877 t
ORIGIN      
        1 tgggcatgct aacaagcggc tggcgcgatt gctgattgcc tggaagctgg aacaacagca
       61 acaggaaaat agcgcggcgc tgaaatcgca gcggcgaatg ttccatcacc agattgaacg
      121 tggcaacccg cgacgaacgt tcacagggat ggcttttatc gaaggataat gaaggatgaa
      181 actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat
      241 ttctttcagc agttcagtca gtagtgtcca gtaatgacct acgctttcga tgtgaacttg
      301 ctcatccgga gagtgtggac cggtgatggt tggcccgata gaaaccatgt ccatttccgg
      361 atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt
      421 cttgttgaac aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc
      481 cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag
      541 cgaatccagc atgctcacca cgtagtcttt accgctgtcg atcagagaac ggatcaggca
      601 gtgaatttct acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac
      661 cacacctttt gccacgtcgg agttgcggat cacaccgttc ggggtggcgt tcagcagacg
      721 aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcaa cagagtccag
      781 caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt
      841 attcaccaga gatttcaaag cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc
      901 ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag
      961 ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac
     1021 gtggatttca ccgccggagt gaccgccttt caggcctttt aaggttaact tgaaggtttc
     1081 aaagccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgatacctcc
     1141 cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc
     1201 ctgcaaccag ttgctctgta agccaaaagc accgtccata ccggcttctt cggtcatggt
     1261 cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga
     1321 ggccatacca atgccgttat ccgcacccag cgtggtgccg cgtgctttaa cccattcgcc
     1381 atcaatataa ggctggatag gatctttggt gaagtcatgc acggtgtcgt tatttttttg
     1441 cggcaccata tcgaggtggg cctgcaagac gaccggttta cgattttcca tacctgcggt
     1501 agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc
     1561 ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat
     1621 agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga
     1681 cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag
     1741 aaaatgttgt caacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     1801 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     1861 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     1921 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     1981 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     2041 ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     2101 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     2161 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtagata
     2221 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     2281 tcgcaaaacc ggctggtcgt ccgctggttg atgattatgt tgttgatatc ccgcaaaata
     2341 cctggatcga acagccgtgg gatatgggcg tcgtattcgt gccgccaatc tccggtcgct
     2401 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     2461 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     2521 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttagtcac
     2581 ggcgcacaac tgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     2641 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cccgtgaaat cctcgacgtc
     2701 caggcgcgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     2761 attggctacc gcggcggcaa ctggatttat gagtgggcta cccaggcgat ggtgtggcaa
     2821 caaaaagcct gtgcggaaga agatccgcaa ctcagcggtc gatactggct gcatgcggct
     2881 acgttgtaca acattgctgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg
     2941 caggctttgt caaaccgcgc ctatgaagag gccgctcagc gtctgccggg cacgatgcgg
     3001 cagatggagt ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa
     3061 ggcgatggcc cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac
     3121 tattacagcc tgtatgaacg ttattttgcg ccgcgcggca ttgctatgct gactattgat
     3181 atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat
     3241 cagcacgttt taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc
     3301 tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt
     3361 ctgaaagtgg ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc
     3421 cagcaacagg tgccggaaat gtatcttgac gttctggcca gtcgtttagg gatgcatgat
     3481 gcttccgatg aagcgttgcg agtggagttg aatcgctatt catt
//
LOCUS       DAZGTJ010000414         1584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.guided.255, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000414 DAZGTJ010000000
VERSION     DAZGTJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.guided.255"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            124..1083
                     /gene="stxA2b"
                     /locus_tag="ACHK8E_005477"
     CDS             124..1083
                     /gene="stxA2b"
                     /locus_tag="ACHK8E_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0347551.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1096..1359
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005478"
     CDS             1096..1359
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0347552.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          425 a          323 c          384 g          452 t
ORIGIN      
        1 ggtcactggt tcgaatccag tacaacgcgc catacttatt ttttctggct cgcttttgcg
       61 ggcctttttt atatctgcgc cgggtctggt gctgattact tcagccaaaa ggaatacctg
      121 tatatgaagt gtatattgtt aaaatgggta ctgtgcctgt tactgggctt ttcttcggta
      181 tcctattccc gggaatttac gatagacttt tcgacccaac aaagttatgt ctcttcgtta
      241 aatagtatac ggacagaaat atcgacccca cttgaacata tatctcaggg gaccacatcg
      301 gtgtctgtta ttaaccacac cccaccgggc agttattttg ctgtggatat acgagggctt
      361 gatgtctatc aggcgcgttt tgaccatctt cgtctgatta ttgagcaaaa taatttatat
      421 gtggccggat tcgttaatac ggcaacaaat actttctaca ggttttcaga ttttgcacat
      481 atatcagtgc ccggtgtgac aactgtttcc atgacaacgg acagcagtta taccactctg
      541 caacgtgtcg cagcgctgga acgttccgga atgcaaatca gtcgtcactc actggtttca
      601 tcatatctgg cgttaatgga gtttagtgga aatgccatga ccagagatgc atccagagca
      661 gttctgcgtt ttgtcactgt cacagcagaa gccttacggt tcaggcaaat acagagagaa
      721 tttcgtctgg cactgtctga aactgctcct gtttatacga tgacaccgga agaagtggac
      781 ctcacactga actgggggag aatcagcaat gtgcttccgg agtttcgggg agagggtggt
      841 gtcagagtgg ggcgaatatc ctttaataat atatcagcga tactgggcac agtggcggtt
      901 atactgaatt gccatcatca gggggcgcgt tccgttcgcg ccgtgaatga agagatacaa
      961 ccagaatgtc agataactgg cgacaggcca gttataagga taaacaatac gttatgggaa
     1021 agtaataccg cagctgcttt tctgaatcgc agggctcact ctttaaatac atccggagaa
     1081 taacaggagt taaatatgaa gaagatattt gtagcggctt tatttgcttt tgtttctgtt
     1141 aatgcaatgg cagctgattg tgcaaaaggt aaaattgagt tctctaagta taatgagaat
     1201 gatacattca cagtaaaagt ggccgggaaa gagtactgga ctaaccgctg gaatctgcaa
     1261 ccgctactgc aaagcgcaca gttaacagga atgacggtaa caatcaaatc aaatacctgt
     1321 gcgtcaggtt caggatttgc tgaagtgcag tttaattaat atcagaagca ttgctggttt
     1381 cgtggtgtgc agcaatgtag ttacagtgta atcaatgtca caattcagtc agttgaaggt
     1441 tgtctgcccg actgagaatt tgttaaaaaa atcctgcatg gtgaatcccc ctgagcggag
     1501 gggcatatca gcgtcacagg tgtttctgtt ttacctctat cctttctgtg cgggttcagg
     1561 tgctgatact gaactcaccg ggag
//
LOCUS       DAZGTJ010000415         1584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.guided.256, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000415 DAZGTJ010000000
VERSION     DAZGTJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.guided.256"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            124..1083
                     /gene="stxA2b"
                     /locus_tag="ACHK8E_005479"
     CDS             124..1083
                     /gene="stxA2b"
                     /locus_tag="ACHK8E_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0347553.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1096..1359
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005480"
     CDS             1096..1359
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0347554.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          424 a          323 c          385 g          452 t
ORIGIN      
        1 ggtcactggt tcgaatccag tacaacgcgc catacttatt ttttctggct cgcttttgcg
       61 ggcctttttt atatctgcgc cgggtctggt gctgattact tcagccaaaa ggaatacctg
      121 tatatgaagt gtatattgtt aaaatgggta ctgtgcctgt tactgggctt ttcttcggta
      181 tcctattccc gggaatttac gatagacttt tcgacccaac aaagttatgt ctcttcgtta
      241 aatagtatac ggacagaaat atcgacccca cttgaacata tatctcaggg gaccacatcg
      301 gtgtctgtta ttaaccacac cccaccgggc agttattttg ctgtggatat acgagggctt
      361 gatgtctatc aggcgcgttt tgaccatctt cgtctgatta ttgagcaaaa taatttatat
      421 gtggccggat tcgttaatac ggcaacaaat actttctaca ggttttcaga ttttgcacat
      481 atatcagtgc ccggtgtgac aactgtttcc atgacaacgg acagcagtta taccactctg
      541 cagcgtgtcg cagcgctgga acgttccgga atgcaaatca gtcgtcactc actggtttca
      601 tcatatctgg cgttaatgga gtttagtgga aatgccatga ccagagatgc atccagagca
      661 gttctgcgtt ttgtcactgt cacagcagaa gccttacggt tcaggcaaat acagagagaa
      721 tttcgtctgg cactgtctga aactgctcct gtttatacga tgacaccgga agaagtggac
      781 ctcacactga actgggggag aatcagcaat gtgcttccgg agtttcgggg agagggtggt
      841 gtcagagtgg ggcgaatatc ctttaataat atatcagcga tactgggcac agtggcggtt
      901 atactgaatt gccatcatca gggggcgcgt tccgttcgcg ccgtgaatga agagatacaa
      961 ccagaatgtc agataactgg cgacaggcca gttataagga taaacaatac gttatgggaa
     1021 agtaataccg cagctgcttt tctgaatcgc agggctcact ctttaaatac atccggagaa
     1081 taacaggagt taaatatgaa gaagatattt gtagcggctt tatttgcttt tgtttctgtt
     1141 aatgcaatgg cagctgattg tgcaaaaggt aaaattgagt tctctaagta taatgagaat
     1201 gatacattca cagtaaaagt ggccgggaaa gagtactgga ctaaccgctg gaatctgcaa
     1261 ccgctactgc aaagcgcaca gttaacagga atgacggtaa caatcaaatc aaatacctgt
     1321 gcgtcaggtt caggatttgc tgaagtgcag tttaattaat atcagaagca ttgctggttt
     1381 cgtggtgtgc agcaatgtag ttacagtgta atcaatgtca caattcagtc agttgaaggt
     1441 tgtctgcccg actgagaatt tgttaaaaaa atcctgcatg gtgaatcccc ctgagcggag
     1501 gggcatatca gcgtcacagg tgtttctgtt ttacctctat cctttctgtg cgggttcagg
     1561 tgctgatact gaactcaccg ggag
//
LOCUS       DAZGTJ010000416         1584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.guided.257, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000416 DAZGTJ010000000
VERSION     DAZGTJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.guided.257"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            124..1083
                     /gene="stxA2b"
                     /locus_tag="ACHK8E_005481"
     CDS             124..1083
                     /gene="stxA2b"
                     /locus_tag="ACHK8E_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0347555.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1096..1359
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005482"
     CDS             1096..1359
                     /gene="stxB2b"
                     /locus_tag="ACHK8E_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0347556.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          425 a          322 c          384 g          453 t
ORIGIN      
        1 ggtcactggt tcgaatccag tacaacgcgc catacttatt ttttctggct cgcttttgcg
       61 ggcctttttt atatctgcgc cgggtctggt gctgattact tcagccaaaa ggaatacctg
      121 tatatgaagt gtatattgtt aaaatgggta ctgtgcctgt tactgggctt ttcttcggta
      181 tcctattccc gggaatttac gatagacttt tcgactcaac aaagttatgt ctcttcgtta
      241 aatagtatac ggacagaaat atcgacccca cttgaacata tatctcaggg gaccacatcg
      301 gtgtctgtta ttaaccacac cccaccgggc agttattttg ctgtggatat acgagggctt
      361 gatgtctatc aggcgcgttt tgaccatctt cgtctgatta ttgagcaaaa taatttatat
      421 gtggccggat tcgttaatac ggcaacaaat actttctaca ggttttcaga ttttgcacat
      481 atatcagtgc ccggtgtgac aactgtttcc atgacaacgg acagcagtta taccactctg
      541 caacgtgtcg cagcgctgga acgttccgga atgcaaatca gtcgtcactc actggtttca
      601 tcatatctgg cgttaatgga gtttagtgga aatgccatga ccagagatgc atccagagca
      661 gttctgcgtt ttgtcactgt cacagcagaa gccttacggt tcaggcaaat acagagagaa
      721 tttcgtctgg cactgtctga aactgctcct gtttatacga tgacaccgga agaagtggac
      781 ctcacactga actgggggag aatcagcaat gtgcttccgg agtttcgggg agagggtggt
      841 gtcagagtgg ggcgaatatc ctttaataat atatcagcga tactgggcac agtggcggtt
      901 atactgaatt gccatcatca gggggcgcgt tccgttcgcg ccgtgaatga agagatacaa
      961 ccagaatgtc agataactgg cgacaggcca gttataagga taaacaatac gttatgggaa
     1021 agtaataccg cagctgcttt tctgaatcgc agggctcact ctttaaatac atccggagaa
     1081 taacaggagt taaatatgaa gaagatattt gtagcggctt tatttgcttt tgtttctgtt
     1141 aatgcaatgg cagctgattg tgcaaaaggt aaaattgagt tctctaagta taatgagaat
     1201 gatacattca cagtaaaagt ggccgggaaa gagtactgga ctaaccgctg gaatctgcaa
     1261 ccgctactgc aaagcgcaca gttaacagga atgacggtaa caatcaaatc aaatacctgt
     1321 gcgtcaggtt caggatttgc tgaagtgcag tttaattaat atcagaagca ttgctggttt
     1381 cgtggtgtgc agcaatgtag ttacagtgta atcaatgtca caattcagtc agttgaaggt
     1441 tgtctgcccg actgagaatt tgttaaaaaa atcctgcatg gtgaatcccc ctgagcggag
     1501 gggcatatca gcgtcacagg tgtttctgtt ttacctctat cctttctgtg cgggttcagg
     1561 tgctgatact gaactcaccg ggag
//
LOCUS       DAZGTJ010000417         1547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2107S20140
            SAMN43267532-rid23077513.guided.259, whole genome shotgun sequence.
ACCESSION   DAZGTJ010000417 DAZGTJ010000000
VERSION     DAZGTJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267532
            Sequence Read Archive: SRR30307405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350723.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350723.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:06:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 75 of 185
            Pseudo Genes (incomplete)         :: 124 of 185
            Pseudo Genes (internal stop)      :: 52 of 185
            Pseudo Genes (multiple problems)  :: 53 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267532-rid23077513.guided.259"
                     /serotype="O146:H28"
                     /isolate="2107S20140"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHK8E_005483"
     CDS             259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHK8E_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP0347557.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHK8E_005484"
     CDS             1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHK8E_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP0347558.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          419 a          264 c          368 g          496 t
ORIGIN      
        1 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
       61 tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
      121 agctctcttt gaatataatt atcattttca ttacgttatt gttacgttta tccggtgcgc
      181 cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa tatagttatc gtatggtgct
      241 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
      301 atcttttcag ttaatgtggt tgcgaaggaa tttaccttag acttctcgac tgcaaagacg
      361 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
      421 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
      481 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
      541 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
      601 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
      661 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
      721 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
      781 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
      841 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
      901 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgac
      961 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
     1021 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
     1081 gcatctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
     1141 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
     1201 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
     1261 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
     1321 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
     1381 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
     1441 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgactcag aatagctcag
     1501 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattc
//
